---
license: cc-by-4.0
pretty_name: Widefield 3D DAPI nuclei with Fucci2a cell-cycle labels (derived from S-BIAD1752)
tags:
- microscopy
- cell-cycle
- fluorescence
---

# Widefield 3D DAPI nuclei with G1 / S/G2 labels

`widefield.tar` holds 4,074 single-nucleus crops of fixed Fucci2a mouse 3T3 fibroblasts stained with DAPI, imaged
on a widefield epifluorescence microscope with a 40x objective (voxels 0.188 x 0.188 x 0.5 micrometres), each
labelled G1 or S/G2 from the Fucci2a reporter.

- `widefield/images/<id>.tif`: DAPI counts, uint16, planes x rows x columns, the nucleus's bounding box padded
  by 1 micrometre (clipped at the tile), zlib-compressed TIFF with the voxel size in its metadata.
- `widefield/masks/<id>.tif`: uint8, 1 on the nucleus's voxels.
- `widefield/labels.csv`: `id,phase,field` (field is the tile of the source acquisition).

## Source and licence

Derived from BioImage Archive **S-BIAD1752** (https://doi.org/10.6019/S-BIAD1752), "Predicting cell cycle stage
from 3D single-cell nuclear stained images", by Gang Li, Eva K. Nichols, Valentino E. Browning, Nicolas J.
Longhi, Madison Sanchez-Forman, Conor K. Camplisson, Brian J. Beliveau and William S. Noble, licensed under
CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/). Paper: Li, Nichols et al., Life Science Alliance
(2025), doi 10.26508/lsa.202403067.

Changes: the DAPI channel of the 40x tiles (`tile_N.tif`) was cropped around each nucleus labelled in
`GT_labels_40x_with_source_file.tsv`, with the nucleus's own mask cut from the deposited filtered segmentation;
intensities are unchanged; nuclei were renamed to random ids. This dataset is released under CC BY 4.0.