A special pattern of the smooth endoplasmic reticulum in the kidney of the snail Cryptomphalus aspersa. A special structural pattern of the smooth endoplasmic reticulum (SER) has been observed in the kidney of the snail Cryptomphalus aspersa. Two types of cells (clear and dark) cover the foldings of the renal sac; the dark cells are by far the most numerous. A cisterna of SER enveloping the nucleus appears invariably in both types of cells, with no disruptions, or small ones (from 50 to 90 nm) along its profile. The layer of cytoplasm lodged between the external nuclear membrane and this cisterna is found invariably to be from 0-20 to 0-25 mum in width. Glycogen is abundant in the cytoplasm as alpha particles, and also in the nucleus, but as beta particles. It is noteworthy that absolutely no glycogen is present in the layer of cytoplasm lodged between the nuclear membrane and the surrounding SER envelope. Long profiles of SER are also observed closely approaching and parallel to the plasma membrane of the dark cells. Considering the role of SER in glycogen metabolism in the kidney of the snail, the possible function of these cisternae as a support system ofr enzymes involved in the metabolism of glucides is discussed.


Phagocytosis of concanavalin A in normal and enucleated cultures of mammalian cells. The fate of concanavalin A (Con A) bound to normal and enucleated L cells was followed at the ultrastructural level over a 20-h period. In both enucleates and normal cells the Con A is seen to be distributed in a uniform manner over the entire cell surface following a 30-min pulse with a low concentration of Con A. In the subsequent chase period the cells then aggregate the Con A and Con A sites into large clusters on the cell membrane. The cells then phagocytoze the Con A and large phagocytic vacuoles containing it are observed. Thus, enucleated cells are capable of phagocytozing Con A and its sites in the same manner as normal cells.


Cytoplasmic streaming in Chara corallina studied by laser light scattering. An apparatus is described by means of which the power versus frequency spectrum of the photomultiplier current can be obtained for laser light scattered by streaming cytoplasm in the algal cell Chara corallina. A Doppler peak is noted in the spectrum which is abolished when cytoplasmic streaming is arrested by electrical stimulation. For 5 cells of Chara, this simple laser-Doppler velocimeter gave streaming velocities (46-7 mum s-1, S.D. +/- 4-8 at 20 degrees C) similar to those obtained for the same cells using the light microscope (44-3 mum s-1, S.D. +/- 5-3 at 20 degrees C). A narrow distribution of streaming velocities is indicated. The technique described provides a rapid, quantitative assay of the in vivo rheological properties of cytoplasm.


Mechanisms of cell adhesion: early-forming junctions between aggregating fibroblasts. When cultured fibroblasts (16C) are mildly dissociated with EGTA or trypsin/EDTA, they aggregate rapidly. The formation of aggregates has been found to involve junctions of the gap and adhaerens types which are seen by electron microscopy within minutes of allowing cells to come together. The process of adhesion between freshly dissociated, transformed 16C fibroblasts is therefore organized and establishes its usefulness as a model for studying cellular interactions in relation to supracellular organization.


Jaw mechanics during release of the stuttering moment: some initial observations and interpretations. As a part of a larger study (Hutchinson and Watkin, 1974), this investigation was undertaken to examine jaw mechanics during the release of stuttering moments. Six adult subjects (four stutters, ranging in severity, and two normal talkers) read a series of sentences containing phonetic sequences requiring jaw depression. Strain-gauge transducers were employed to record articulatory movement. Only those stutterings occurring immediately prior to and during jaw opening were studied. The results confirmed generally faster jaw velocities for the opening and closing gestures; which was interpreted to suggest that the spindle motor system is maximally active to preserve synchrony in on-going vocal tract events following the dysfluency. In cases where an asynchrony between vocalization and jaw opening was noted, it was suggested that imprecision in predicting voice onset resulted in articulatory-laryngeal incoordination. It would appear from these interpretations that the clinical efficacy of the "pull out" procedure results from reduced demands on the gamma loop system and facilitation of articulatory-laryngeal synchrony through reduced supraglottal velocities.


Assay of intercellular adhesiveness using cell-coated Sephadex beads as collecting particles. A simple, rapid and precise method, based on a previous method, for measuring relative rates of intercellular adhesion is described. DEAE-Sephadex beads were treated with nitrocellulose in order to allow cells to grow on their surfaces. Balb/c 3T3 and Balb/c 3T12 cells were used to characterize the assay. They formed confluent cell layers on nitrocellulose-treated DEAE-Sephadex. These cell-coated beads were employed to collect 32P-labelled cells from single cell suspensions. Since they formed statistically uniform, large collecting surfaces, the collection of labelled cells was markedly improved as compared to the original assay. The cell-coated beads collected a large percentage of the labelled cells in a short time. The percentage of cells collected was independent of the concentration of labelled cells in the assay mixture, and the collection was linear for approximately 60 min. The variability between replicate assays was usually +/- 5%. The assay allows the rapid and precise determination of intercellular adhesion in large numbers of individual samples. These features make it useful to screen for effects of different treatments on intercellular adhesions.


Traumatic aphasia in children: a case study. Traumatic aphasia in children has been recognized as a distinct clinical pathology, differing from adult aphasia in symptomatology and course of recovery. The upper limit for complete recovery has been identified as age 10. However, there is a paucity of literature documenting recovery of children with traumatic aphasia. It is apparent that definitive statements of the upper age limit for complete recovery from traumatic aphasia in children cannot be made at this time. This article reviews the literature concerning traumatic aphasia and presents case information of a 10-year-old traumatic aphasic girl seen at the North Texas State University Speech and Hearing Center. The design, execution, and assessment of therapeutic interaction and the observed language abilities of the client are reported.


Relationship of clinician feedback to child initiated verbalization during language training. A systematic observational procedure was used to obtain frequency counts of client and clinician initiated interchanges and clinician feedback during these interchanges. Repeated recordings were made of eight different speech pathologists interacting with two groups of four preschool children. Child responses to clinician initiated interchanges received significantly more positive feedback than child initiated verbalizations to the clinician. However, considerable variation among clinicians was found both in the frequency of spontaneous verbalizations by their clients and their reaction to this verbalization. Stepwise multiple regression analyses indicated that the type and amount of clinician feedback for client initiated verbalizations was significantly related to the frequency of these utterances to the clinician, but not to other children.


Cognitive conditioning therapy in the treatment of stuttering. A therapy is described in which clients vividly imagine the cognitions that usually trigger their stuttering, and then contingently experience a painful shock. With the cessation of shock, alternate desirable cognitions are imagined that are designed to facilitate fluent speech. A case study illustrating the treatment is presented. Stuttering is compared with addictive behaviors such as smoking, and it is concluded that cognitive commonalities exist among seemingly disparate behaviors. The cognitive conditioning framework provides an integrative model that can be usefully applied in a variety of situations.


Perceptual and acoustic analysis of repetitions in stuttered speech. This study presents perceptual and acoustic data on a carefully selected set of part-word repetitions from the speech of adult stutters. Results indicated that the schwa vowel was perceived in only 25% of the repetitions, far less than previously indicated. Spectrographic analysis showed that although abnormal consonant duration and C-V formant transitions characterized the initial segment of the stuttered word, the remainder of the word is identical to its identical to its fluently produced counterpart. The results were interpreted to mean that for the type of dysfluency selected, the articulatory breakdown is confined to the initial consonant, and it is likely that abnormal formant transitions from initial consonant to vowel, when present, are due to deviant formation of the consonant rather than to faulty transition dynamics.


A refinement of the concept "reticence". This report has 1. Made a distinction between speech disorders involving defects and those that are primarily reticence, but in any case identified speech disorders as negotiated states as opposed to fixed disease states. 2. Argued that there is a clear analytical separation between speech disorders and speech behaviors which are indicative of neurotic disorders. 3. Attempted to remove anxiety as a component of the communication process by according it its rightful place, which is central in human personality. Furthermore, we have attempted to dispel the notion that anxiety is evil and the perennial (and sole) cause of speech disorders. 4. Identified a specialist known as the rhetoritherapist (a specially trained speech teacher) as the particular king of professional qualified to deal with speech disorders (in conjuction with speech pathologists and/or psychotherapists where necessary). We have accorded to the rhetoritherapist the province of instruction and training in all aspects of invention, delivery, and reception of rhetorical speech without reference to its moral intent. 5. Identified "reticence" as the most useful of the various imprecise terms used to refer to people with speech problems, because it is devoid of connotations that go beyond the speech process. Further refinement of specific categories of speech disorders is necessary to order to expand the repertoire of available treatment strategies. Such refinement will probably include reference to the various subprocesses of human speech identified earlier in this paper as they are related to the rhetorical situation. The rhetoritherapist thus emerges as the trouble-shooter, but not the "psychotherapist," of speech pedagogy.


Giant and "granular melanosomes" in Leopard syndrome: an ultrastructural study. Electron microscopy of lentigines was performed to study the pigmentation abnormality in two children with LEOPARD syndrome. Giant melanosomes similar to those seen in café-au-lait spots of neurofibromatosis and nevus spilus were found in a lentigine from one of our cases. Our results show that "spherical granular melanosomes" described in neurofibromatosis, are lysosomal-like structures associated with the development of complex melanin granules. Our study also demonstrates that immature melanosomes are present in some keratinocytes of LEOPARD syndrome. This finding is in contrast to the prevailing concept that only mature melanosomes are transferred to keratinocytes. The occurrence of individual melanosomes of normal size and shape in keratinocytes of skin in whites with LEOPARD syndrome, suggests that neither the size of melanosomes, nor the racial differences are the factors determining the distribution of melanosomes in keratinocytes.


Lichenoid benign keratosis.

Lichenoid benign keratosis. One hundred thirty-eight patients who presented with solitary cutaneous lesions appearing clinically as papules or plaques and histologically showing a lichenoid tissue reaction were studied. Many of the lesions occurred in sites not usually exposed to sunlight and histologically the lesions did not show significant atypical change in keratinocytes and many did not show evidence of solar elastosis in the superficial corium. Our study indicates that the lesions are benign keratoses showing a lichenoid tissue reaction or lichenoid benign keratoses.


Dermal changes in osteoporosis following prolonged treatment with human growth hormone. Five patients with osteoporosis were treated with human growth hormone (hGH) for a year and the changes in their skin were studied by light and electron microscopy. The abnormally thin skin of osteoporosis appeared to change towards normal after treatment with hGH. There was a consistent proliferation of blood vessels, and increased number of mast cells and fibrocytes. The collagen bundles and elastic tissue fibers appeared hyperplastic and more horizontally oriented. The fine, vertical elastic fibrils of the papillary dermis had appeared decreased before treatment, but seemed to be restored to their normal configuration after treatment. Since there was no evidence of stimulation of hair, sebum, or melanin such as occurs in acromegaly, it is suggested that the scope of the direct action of hGH on the skin is limited to mesenchymal structures.


Deposition of complement in the lesions of experimental cutaneous candidiasis in guinea pigs. Deposits of C3 have been demonstrated at the dermo-epidermal junction in the lesions of experimental cutaneous candidiasis in guinea pigs. The deposits were granular in character and similar to those previously found in the lesions of chronic mucocutaneous candidiasis in humans. Immunoglobulin, C4 and candida antigen were not detected in the lesions. C3 was also found in a similar pattern at the dermo-epidermal junction of candida-infected skin from homozygous C4-deficient guinea pigs. From these observations, it is postulated that complement deposition occurs in these lesions as a result of alternative pathway activation, perhaps by cell wall components from the infecting organisms.


Transfollicular extrusion of sebaceous glands: natural phenomenon or artifact? A case report. A sebaceous gland tumor on the back of a 28-year-old man underwent periodic exudation of yellowish material in association with local irritation. Pathological examination revealed entire sebaceous gland lobes lying subcorneally in the orifices of sebaceous follicles. This case appears to represent transfollicular extrusion of sebaceous gland lobes as a natural phenomenon rather than as an artifact.


Condylomata acuminata.

Condylomata acuminata. A 5-year-old Thoroughbred chestnut mare was presented because of multiple lesions noticed for approximately one year over the vulva and ther perianal areas. Clinically, there were moist warts that stood out because of their whitish color which contrasted strikingly with the surrounding, normally pigmented, uninvolved skin (Fig. 1). Repeated topical applications of 20% podophyllin in 95% ethyl alcohol produced rapid involution. Histopathologically, marked acanthosis, numerous mitoses, prominently vacuolated epidermal cells and a chronic dermal inflammatory infiltrate were seen (Figs. 2, 3, and 4). Interestingly, melanin in the basal cell layer was not visible in the lesions, but was very apparent in the normal epidermis at the edges of the lesions. Thus the contrast between the affected and the non-affected skin was also strikingly microscopically (Fig. 2 arrows).


Glaucoma crossroads.

Glaucoma crossroads.


Clinical and theoretical considerations of a patient with narrow angles and shallow anterior chambers. Clinicians must always be on the alert to identify those individuals who appear to be anatomically disposed to narrow angle glaucoma. However, it is not axiomatic that these individuals can be provoked into an attack even if a history of subacute attacks exists. In this paper, and in one other to follow, the case histories of several interesting patients that were seen by the author for routine eye examinations will be discussed. The intent of the papers is to demonstrate that the diagnosis of glaucoma, existent or potential, can be quite time consuming and is dependent on far more than just tonometry.


Adverse reactions to topical ocular anesthetics. The purpose of this article is to clarify some of the misunderstanding about adverse reactions to topical ocular anesthetics. Discussion will include: types of adverse reactions that are theoretically and practically possible; advice on handling the reactions that can occur; and precautions for minimizing their occurrence.


Protective spectacles for retinitis pigmentosa patients. On the hypothesis that ophthalmic filters with appropriate light transmission characteristics may be beneficial in retarding the progress of retinal degeneration in early retinitis pigmentosa, the characteristics of several plastic and glass filters have been evaluated. A dark brown plastic goggle has been found which meets the theoretical criteria previously postulated for such protection. Its characteristics and those of other filters are compared with a lens which was specially designed for RP protection and was previously reported on by Adrian and Schmidt.


Modification of Welch-Allyn "11500 ophthalmoscope for visuoscopy. Visuoscopy is an indispensable test in the evaluation of the monocular fixation pattern. Unfortunately, the instrumentation needed to accurately perform the test is often costly. A procedure describing how the Welch-Allyn "11500 ophthalmoscope may be inexpensively modified for the routine performance of visuoscopy is presented.


An evaluation of the use of topical anesthetics and low concentrations of phenylephrine HCl for mydriasis. When used in conjunction with a topical anesthetic, low concentrations of phenylephrine HCl produce the same degree of mydriasis as is produced by higher concentrations of the drug used alone. Contrary to previously published reports, however, there is no evidence to suggest that the use of lower concentrations lessens the risk of adverse reactions. The analysis of available information given here suggests that the risks may in fact be increased when an anesthetic is used.


Accommodative blur in pilocarpine-treated glaucoma. Accommodative blurring of distance visual acuity is a common problem among young patients being treated for glaucoma with pilocarpine. A single case is reported in which a clip-on prescription was given to alleviate such problems. The development of accommodative blur following instillation of pilocarpine is monitored. The dioptric changes required to restore distance acuity to 20/20 are also monitored.


Polaroid photomacrography using the SX-70 camera. This paper describes a technique for taking color photographs of the external eye using the Polaroid SX-70 camera and a biomicroscope. It is particularly useful in providing a photographic record of anomalies such as pterygium, in which the rate of progression across the cornea can be observed over a period of months or years.


Neurological syndromes involving the visual system. Because optometrists are the primary deliverers of eye care, their role in pathology detection is of primary importance. The neurological syndromes of the visual system have been reviewed and put into a chart format as an aid to the optometric clinician.


The differential diagnosis of fundus pathology,.


Inhibition of adenylate cyclase by arsenite and cadmium: evidence for a vicinal dithiol requirement. The effect of sodium arsenite and cadmium chloride on adenylate cyclase activity was examined in turkey erythrocyte membranes. Sodium arsenite was a weak inhibitor of adenylate cyclase -7mM produced only 60% inhibition. Its effect, however, was greatly potentiated by equimolar 2,3 dimercaprol- wherein 0.7 mM sodium arsenite inhibited 100% with an apparent Ki of 0.1 mM. Equimolar mercaptoethanol was less effective in potentiating sodium arsenite inhibition. Thus 0.7mM sodium arsenite in the presence of equimolar mercaptoethanol inhibited adenylate cyclase 56%. Excess 2,3 dimercaprol reversed inhibition by sodium arsenite or cadmium chloride. Sodium arsenite or cadmium chloride inhibited all forms of adenylate cyclase activity tested, including nonhormonal stimulation. Equimolar sodium arsenite and dimercaprol, at concentrations that caused 100% inhibition of adenylate cyclase activity, reduced the binding of the beta-receptor specific ligand iodohydroxybenzylpindolol by less than 15%. These results suggest that turkey erythrocyte membranes contain closely juxtaposed thiol groups and that interaction of such groups with arsenate interferes with the catalytic function of adenulate cyclase.


Advanced retinitis pigmentosa: a case report.


The ligaments of the wrist.

The ligaments of the wrist. The ligaments of the wrist were studied by dissecting ten fixed and seven fresh frozen wrists. In three other specimens multiple cross-sections were prepared. These studies show that the wrist ligaments can be classified into two groups: extrinsic and intrinsic. In the extrinsic group, the deep volar radiocarpal ligaments are three strong and very important structures connecting the radius to the capitate, the radius to the lunate, and, in a deeper layer, the radius to both the scaphoid and the lunate. A ligamentous deficiency was noted frequently between the capitate and the lunate. There also are very strong volar connections between the radius and the medial or ulnar carpus. These studies suggest that certain patients with a generalized ligamentous laxity and weakness will develop a pathological disruption of the volar ligaments with trauma. These torn volar ligaments should be repaired or reconstructed, for repair of only the dorsal ligaments seldom will provide good stability to such wrists.


Comparison of histologic and functional recovery after peripheral nerve repair. After repair, nerve stains of skin and subcutaneous tissue biopsies of 23 fingertips contained identifiable axons in all patients. The level of recovery was more advanced in some patients than in others, as manifested by identifiable nerve in various plexuses and receptors. Meissner's corpuscles were observed to be reinnervated in 12 of 17 patients and in 16 of 23 fingertips. The degree and level of reinnervation did not correlate with clinical testing or subjective impression of the result. The authors conclude that sensibility is the result of several factors active in the peripheral and central nervous system. Axonal regrowth is but one of these.


Restoration of strong grasp and lateral pinch in tetraplegia due to cervical spinal cord injury. Patients with tetraplegia who have "strong" sixth cervical neurologic (C-6) function often can be given active grasp and strong lateral pinch by tendon transfers and tenodeses. Wrist control can be retained by the extensor carpi radialis brevis and flexor carpi radialis and can permit transfer of the extensor carpi radialis longus to provide finger flexion. Either the brachioradialis or pronator teres then is available for transfer to restore adduction-opposition of the thumb with an in situ tendon graft of a paralyzed flexor superficialis rerouted to the thumb through a palmar fascial pulley. The other motor can provide thumb flexion for strong lateral pinch. Extrinsic extension can be provided by tendoeses. With seventh cervical neurologic (C-7) function retained, active digital extension is present and functional expectations are better. Ten hands in seven patients with traumatic tetraplegia from injuries at C-6 or C-7 level have been reconstructed. The average grasp and pinch force after operation was 5.5 and 3.0 Kg., respectively. All patients but one were pleased with the increased function a


The importance of middle or long finger realignment in ulnar drift. Based upon the hypothesis that ulnar drift is the result of a chain reaction starting with a medial shift of the proximal phalanx at the metacarpophalangeal joint and progessing to subluxation of the extensor tendon and finally subluxation of the metacarpophalangeal joint, the author recommends early operative realignment. The extensor tendon is partially divided and a portion passed through the base of the phalanx, the intrinsic tendons are transferred to divided, the junctura tendnium is served, the periosteum is stripped from the neck of the metacarpal, and areas of bony erosions are curetted. In 25 patients with involvement of the middle finger only, alignment was maintained in all over a period of 3 months to 7 years, the average follow-up period being 1.4 years. When deformity was severe and the radical collateral ligament has been disrupted, correction was not always maintained. Early treatment is recommended.


Squamous cell carcinoma of the nail bed. Twenty-seven cases of squamous cell carcinoma of the nail bed seen by the author have been reviewed. Sixteen were thought to be primary tumors and eleven to be secondary to chronic exposure to radiation. All had been present for a long time prior to definitive treatment. The tumor was of low grade malignancy with a tendency to invade the distal phalanx. No metastases have been found. Treatment was amputation of the distal phalanx and involved tissues.


Digital nerve grafts with the lateral antebrachial cutaneous nerve. The lateral antebrachial cutaneous nerve was used as a nerve graft to bridge a defect in a digital nerve, when with the digit in full extension the nerve ends could not be approximated without tension. Thirteen grafts have been followed from 7 to 23 months. Two patients did not regain two point discrimination and therefore had a poor result. The remaining 11 patients recovered two point discrimination between 7 and 20 mm.


Rate and extent of functional recovery after flexor tendon grafting with and without silicone rod preparation. Forty-six consecutive patients requiring flexor tendon grafts were evaluated at 4, 6, 8, 10, 12, 16, 20, 22, 24, 28, 34, and 52 weeks after grafting. The patients were divided into two groups: those who did not require a silicone rubber rod prior to tendon grafting (Group I), and those who did require a silicone rubber rod prior to grafting (Group II). Analysis of the rate of functional recovery as measured by total active motion, gross grip strength, and pinch grip strength showed no significant difference between the two groups. Analysis of the extent of functional recovery at one year, as measured by total active motion and distance of digital pulp from distal palmar crease, showed no significant differences between Groups I and II.


Patterns of neuromuscular activity following tendon transfer in the upper limb: a preliminary study. Early results of a continuing study of the electrical activity of muscles after transfer, with an integrated system of electromyographic and video tape recording, recorded motions of the simple voluntary type, mostly with isometric contractions. A triceps-to-biceps transfer showed activity in the new motion one day after operation. A partial transfer of the pectoralis major (Clark) demonstrated isolated activity of the transfer after one year. A superficialis transfer to clawed ring and little fingers functioned to flex the metacarpophalangeal joints and to extend the interphalangeal joints. Transfer of superficialis to finger extensors showed that antagonists acted to provide unresisted extension. The original phasic activity of a muscle was retained in a study of extensor indicis proprius to thumb for opposition. New phasic activity was shown in transfer of extensor carpi radialis longus to digital flexors. In two instances of transfer of spastic wrist flexors to wrist extensors, the original phasic activity was retained, but in only one was the function assumed by the transfer even though wrist extension was improved.


The abducted little finger in low ulnar nerve palsy. In 12 dissections the extensor digiti minimi tendons inserted into the abductor tubercle of the proximal phalanx. In eight of the 12, the tendons passed to the ulnar side of the abductor-adductor axis of the metacarpophalangeal joint. A separate tendon from the extensor digitorum communis to the little finger was found in only one dissection, with all others showing a connecting band which varies in its obliquity and thus in its adducting effect. To correct an abducted little finger, the ulnar portion of the extensor digiti minimi was transferred to the radial side of the little finger. Two methods were used: (1) the tendon was passed to the volar side of the metacarpophalangeal joint, if the joint could be hyperextended; if not, (2) a simple transfer to the radial collateral ligament was performed.


Proximal row carpectomy with muscle transfers for spastic paralysis. Fourteen patients with spastic paralysis from various causes (eight with cerebral palsy) who needed minimal two handed activities to assist in self-care and who desired improvement in appearance were treated by proximal row carpectomy. In twelve transfer of flexor carpi ulnaris to extensor carpi radialis brevis was done, and in two the extensors of the wrist were shortened. Various other procedures were done in some patients. Prolonged splinting was carried out. Better extension of the wrist was obtained and supination improved more when the transfer was subcutaneous around the ulnar border than when through the interosseus membrane. Less tendency for the carpus to displace ulnarward was seen when the distal half of the scaphoid was not removed. Strength of grasp and pinch improved, but ability to release objects was diminished due to the more dorsiflexed position of the wrist. Subjective use for two handed activities was improved and the patients were satisfied with the appearance.


The pronator quadratus in motions and in stabilization of the radius and ulna at the distal radioulnar joint. Based upon the findings of dissections of 15 forearms and clinical observations in patients with disturbed architecture of the distal radioulnar joint, the pronator quadratus was found to be composed of two heads. A superficial head originated from the ulna and passed transversely to its insertion into the radius. It averaged 5.1 cm. in length, 4.5 cm. in width, 0.2 cm. in thickness, with a cross-sectional area of 0.95 cm.2 and a contractile volume of 2.6 cm.3. The deep head ran obliquely from a more proximal origin on the ulna to a distal insertion on the radius, with an average length of 4.0 cm., average width of 3.2 cm., and a thickness of 0.4 cm. Its average cross-sectional area was 1.64 cm.2 and its contractile volume 2.5 cm.3. A group of fibers occasionally was noted deep to both heads, running at right angles to them and paralleling the direction of the fibers of the interosseous membrane. The superficial head initiates pronation while the deep head coapts the joint surfaces and stabilizes the joint.


Opera-glass hand in rheumatoid arthritis. Characteristic deformities occur in the fingers, thumb, and wrist in the opera-glass hand in rheumatoid arthritis. Shortening and instability are the result of bone resorption and dislocation and can be severely disabling. Early spontaneous fusion of the proximal interphalangeal joint preserves digital length. Functional improvement can be obtained in the fingers by interphalangeal joint arthrodesis and metacarpophalangeal prosthetic arthroplasty and in the thumb with metacarpophalangeal and/or interphalangeal arthrodesis. With interphalangeal arthrodesis, interposition grafts often are required in order to restore length and secure fusion. "Prophylactic" arthrodesis of interphalangeal joints should be considered when resorption seems imminent.


Volkmann's ischemic contracture due to soft tissue injury alone. Fifteen upper extremities, in 14 patients in whom incipient or actual Volkmann's ischemic contracture was present, were seen in a 5 year period. Nine patients were stuporous due to drug overdose and had laid on the extremity; two had received a recent injury of main arterial trunks; two had sudden severe compression; one with chronic myelogenous leukemia had each arm involved at different times in a bizarre autoimmune response causing massive swelling. No patient had a fracture or dislocation. Pain and tenderness, loss of sensibility, resistant muscle contracture, and rock-hard muscle compartments were warning signs. Immediate fasciotomy was done. Useful function was restored when treatment was carried out in the early stages of the ischemia.


Digital vessel trauma from repetitive impact in baseball catchers. The impact to the catcher's hand in baseball was studied. Modification or additional padding in the glove seems indicated to prevent vascular injury.


The dorsal ganglion of the wrist: its pathogenesis, gross and microscopic anatomy, and surgical treatment. During a period of 25 years, 500 dorsal ganglions of the wrist were treated surgically. Three hundred and forty-six were followed for a minimum of 9 months; there were three recurrences. Dissection of the cysts under magnification of six to 25 times and serial microscopic studies showed evidence of a one way, valvelike system between the scapholunate joint and the ganglion. Stress, as a cause of ganglions, is suggested. Operative treatment involved excising all attachments to the scapholunate ligament.


Histoenzymological mapping of acetylcholinesterase and butyrylcholinesterase in the diencephalon and mesencephalon of Uromastix hardwickii. The paper deals with a detailed histochemical mapping of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in the diencephalon and mesencephalon of Uromastix hardwickii. In AChE in contrast to BChE preparations, most of the nuclei reveal intense activity Interestingly, the hypothalamic nuclei demonstrate positive reactions to AChE but totally negative reactions in BChE preparations. The enzymatic activity in fiber tracts is totally negative for AChE; on the contrary, it is intensely positive for BChE. Neurosecretory substance, in the hypothalamic region, is positive for the two preparations of the enzymes. The role of these enzymes, as related to the functional activities of the various nuclei and fiber tracts, has also been discussed.


Age-status labeling in homosexual men.

Age-status labeling in homosexual men. Ninety-five homosexual men between 25 and 68 years of age were asked to classify themselves as young, middle-aged, or old. Most men in their 20s and 30s described themselves as young; most men in their 40s and all men over 50 described themselves as middle-aged. The popular suggestion of accelerated aging in homosexual men was not supported. Homosexual men who anticipate such accelerated aging might possess poorer physical and or psychological health than those who do not.


Differences in psychological sex, adjustment, and familial influences among homosexual and nonhomosexual populations. The present study investigated differences in psychological sex, present and past adjustment, and parental influences among homosexual cross-dressers, homosexual non-cross-dressers, applicants for sex change surgery, and heterosexuals. The homosexual non-cross dresser and heterosexual groups were found to have the most masculine gender role, with the sex change group having the most feminine gender identity. The two homosexual groups were most accepting of homosexuality, with the sex change group having the least acceptance of homosexuality. Support was not found for the prediction that the sex change group would have the worst present and past adjustment followed by the homosexual cross-dressers with the poorest past adjustment. As predicted, however, fathers were perceived as more nurturant in the heterosexual group than among the remaining three groups. These findings suggest that variations in sexual-life-style can be understood as manifestations of different combinations of the components of psychological sex and that a nurturant father is important in the development of a heterosexual life-style.


Homosexuality in the Rorschach: a new look at the old signs. Content responses to the Rorschach inkblot test have been used by clinicians to assess homosexuality. In comparing educated, adjusted male homosexuals to educated, adjusted heterosexuals, no differences were found between the groups when the traditional index of homosexuality on the Rorschach was used. The index was unable to distinguish the groups on a number of criteria. The reliability of the index was found to be significantly greater for the homosexual group than for the heterosexual group. It was suggested that the traditional index is not valid and that it should not be used in a clinical setting as a measure to assess homosexuality.


The effects of a homophile organization on the self-esteem and alienation of its members. Wth the expanding number and kinds of homophile organizations, a need exists to evaluate the effect these organizations have upon their members. Stemming from a previous investigation by the author, this study of the relationship between membership in a homophile organization and the dependent variables Self-Esteem and Alienation was conducted. It was found that Self-Esteem remained unaffected while Alienation levels decreased initially but started rising again a 1-year period of participation in the organization. The author hypothesizes reasons for these relationships and suggest areas for subsequent investigations.


Iatrogenic homosexuality: gender identity in seven 46,XX chromosomal females with hyperadrenocortical hermaphroditism born with a penis, three reared as boys, four reared as girls. This paper describes seven chromosomal and gonadal females with the adrenogenital syndrome who were born with a penis as a result of extreme fetal androgenization. Four of them were reared as girls and differentiated a female gender identity with tomboyism. The other three were reared as boys, differentiated a male gender identity, and performed sexually as men with women partners. Even though these men are by no means homosexual in the everyday meaning of the term, the sexual relation is homosexual on the criteria of chromosomal and gonadal sex. The prenatal hormonal environment as well as the social experience of the rearing have thus demonstrated a formula for creating the perfect female homosexual.


The use of stimulus/modeling videotapes in assertive training for homosexuals. The purpose of this study was to develop and apply an experimental approach for teaching assertive behaviors in selective problematic interpersonal situations encountered by homosexuals. Specifically, the approach explicates a treatment format that combines the use of stimulus/modeling videotapes with behavioral rehearsals, videotape feedback, and home assignments. Results of a single-subject interrupted time-series analysis suggest that the client displayed significantly more assertive behaviors on four of the six criterion measures. The findings further suggest that with contextual modification this interventive format can be applied to a variety of sexual behavior patterns.


Code switching and sexual orientation: a test of Bernstein's sociolinguistic theory. Bernstein's theory was tested in the homosexual's "closed" community to determine code-switching ability and its relationship to jargon. Subjects told a story based on homoerotic photographs where knowledge of sexual orientation was varied. Rather than finding the restricted code associated with in-group communication, an analysis of data trends (since all hypotheses were rejected) suggests that homosexual hemophyly encouraged elaboration, and status differentiation resulted in a more restricted code. Story length was the most significant variable across groups. Some of Bernstein's theoretical explanations require modification to account for subjects' behavior in stigmatized social groups.


Forbidden colors of love: patterns of gay love and gay liberation. A typology of styles of loving is applied to the search for partners among gay males, using a sample of advertisers in a gay newspaper. Characteristics of advertisers and of the partners they seek are analyzed. The impact of gay liberation ideology on gay male relationships is considered as a model of predicted changes in heterosexual male selection patterns.


Reported consequences of decriminalization of consensual adult homosexuality in seven American states. This article reports results of a survey of police officials, prosecuting attorneys, and members of homosexual groups in the seven states that had decriminalized private homosexual behavior between consenting adults. Despite the dire predictions of many, the responses indicate that, among other things, decriminalization has had no effect on the involvement of homosexuals with minors, the use of force by homosexuals, or the amount of private homosexual behavior. Additionally, decriminalization reportedly eased somewhat the problems of the homosexual community and allowed the police to devote more time to the investigation of what generally are regarded as more serious criminal offenses.


The role of cognitive and situational variables in aggression toward homosexuals. The relationship of several antecedents of aggression toward homosexuals was investigated. Attitudes toward homosexuality, perceived similarity to the target homosexual, and type of prior contact with the target homosexual were found to interact in influencing such aggressiveness. The implication of these findings to the "personal threat" and "scapegoating" hypotheses of aggression toward homosexuals is discussed.


Sexual aspirations and sexual behaviors among homosexually behaving males and females: the impact of the gay community. Questionnaires were administered to 100 males and 168 females who expressed strong homoerotic aspirations. These two groups were further divided into those who were members of the gay community and those who were not. Two questions served to focus the research effort: (a) to what extent is the relationship between homoerotic sexual aspirations and subsequent gratifications different for homosexuality behaving females and males, and (b) what, if any, effect does membership in the gay community have on the sexual facilitation of these aspirations? Analysis of the data suggests that issues pertaining to differences between homosexually behaving males and females cannot be addressed apart from corresponding issues concerning the impact of the gay community as a facilitator of homoerotic sexual aspirations.


A factor-analytic conceptualization of attitudes toward male and female homosexuals. A wide spectrum of opinions and beliefs concerning homosexuals was sampled, compiled into a questionnaire format, and administered to a large group of heterosexual subjects. Factor analysis yielded six independent sets of attitudes that describe the variance in heterosexual reactions to homosexuals. The relationship of sex differences and familiarity with homosexuals to variations in response style is discussed.


Homophobia: the quest for a valid scale. Using a modified version of Smith's "homophobic scale", this study examines attitudinal differences between 60 homosexual and 60 heterosexual Caucasian, middle-class males. While Smith's items fail to meet minimal Guttman Scalogram requirements, significant attitudinal differences were recorded between groups. Furthermore, homosexual subjects were significantly more liberal than heterosexual subjects regarding the propriety of masturbation extramarital sexual activities, as well as the sexual activities of their teenage sisters. Although homosexual subjects evidenced a consistent liberal attitude toward sexual behavior in general many heterosexuals indicated a strong margin of attitudinal uncertainty in their responses.


Case management of the gender incongruity syndrome in childhood and adolescence.


Why do women liver longer than men?

Why do women liver longer than men? In the contemporary United States, mortality is 60% higher for males than for females. Forty percent of the excess of male mortality is due to arteriosclerotic heart disease, which is more common among men in part because they smoke cigarettes more than women do, and apparently also because they more often develop the competitive, aggressive Coronary Prone Behavior Pattern. Men who do not develop this Behavior Pattern may have as low a risk of coronary heart disease as comparable women. Oophorectomy of young women may increase the risk of coronary heart disease, but administration of female hormones generally does not reduce risk. One third of the sex differential in mortality is due to men's higher rates of suicide, fatal motor vehicle and other accidents, cirrhosis of the liver, respiratory cancers and emphysema. Each of these causes of death is linked to behaviours which are encouraged or accepted more in males than in females: using guns, drinking alcohol, smoking, working at hazardous jobs, and seeming to be fearless. Thus, the behaviors expected of males in our society make a major contribution to their elevated mortality.


Task demand as reflected in catecholamine excretion and heart rate. Immediate effects and aftereffects of exposure to a color-word conflict task were studied in two groups of subjects, one of which performed the task without auditory interference ("single conflict"), the other with auditory interference ("double conflict"). Physiological arousal indices were more susceptible than performance measures to the level of task demand. Thus, the higher demand imposed by the double-conflict task was reflected in relatively larger increases of adrenaline excretion and heart rate, both during the conflict task and during the subsequent arithmetic task, whereas the performance measures remained unaffected.


Lesbian/feminist orientation among male-to-female transsexuals. Autobiographical case studies of two male-to-female transsexuals reveal a pattern of strong female identity couples with lesbian sexual-affectional preference and feminist value orientation. For both informants there is evidence of social concern and activism from an early age, which led them to perceive their gender dilemmas in a political as well as personal way. This case material challenge the notion that transsexuals are necessarily "sexualy Uncle Toms" and demonstrated that male-to-female transsexuals (like genetic women) can achieve an affirmative lesbian identity; it suggests more generally that the development of sexual identity is an ongoing, lifelong process.


Health patterns associated with type A behavior: a managerial population. Type A Behavior is a behavioral syndrome found to be related to coronary heart disease and characterized by excessive drive, ambition, and competitiveness. Managers from 12 different companies were examined for this syndrome and for a number of the known risk factors in coronary heart disease (blood pressure, cholesterol, triglycerides, uric acid, smoking, and fitness). Those individuals exhibiting extreme Type A Behavior (Type A) showed significantly higher blood pressure (systolic and diastolic) and higher cholesterol and triglyceride levels. A greater percentage of these individuals were cigarette smokers. On serum uric acid there were no differences. In each age group, Type A's were less interested in exercise, although differences in cardio-respiratory fitness were found only in the oldest age group. Type A Behavior also was related to age, education, company growth rates, and stress symptoms. Overall, the Type A1's were found to be higher on a number of risk factors known to be associated with coronary heart disease. With regard to the Type A2's (individuals with less developed Type A Behavior), the findings were not conclusive.


Effect of stress due to anticipated minor surgery upon in vivo platelet aggregation in humans. When measured just prior to hospital admission, platelet aggregation was faster and systolic and diastolic blood pressure were higher compared to measurements just prior to surgery and upon discharge fromthe hospital.


Why do women live longer than men?

Why do women live longer than men? In the contemporary United States, males have 60 percent higher mortality than females. In Part I, published in the previous issue, we showed that 40 percent of this sex differential in mortality is due to a twofold elevation of arteriosclerotic heart disease among men. Major causes of higher rates of arteriosclerotic heart disease in men include greater cigarette smoking among men; probably a greater prevalence of the competitive, aggressive Coronary Prone Behavior Pattern among men; and possibly a protective role of female hormones. In addition, men have higher death rates for lung cancer and emphysema, primarily because more men smoke cigarettes. In Part II we analyze the other major causes of men's higher death rates: accidents, suicide, and cirrhosis of the liver. Each of these is related to behaviors which are encouraged or accepted more in men than in women in our society--for example, using guns, being adventurous and acting unafraid, working at hazardous jobs and drinking alcohol. We conclude with suggestions for reducing male mortality; for example, by changing the social conditions which foster in men the behaviors that elevate their mortality.


Stress, illness, and illness behavior.

Stress, illness, and illness behavior. This paper examines adaptation as a transactive process involving the skills and capacities of individuals and their supporting groups on the one hand, and the types of challenges they face on the other. Many difficulties in understanding stress processes in illness result from the confusion between illness and illness behavior. It is argued that the medical record is as much a history of the individual's behavior and social selection processes as it is a reflection of levels of physical health. Various examples are discussed, illustrating how medical records can be misleading in research examining the relationship between stress and illness, and how influences attributed to stress may be the result of illness behavior. The paper concludes by examining alternative conceptual models for studying the relationships between life challenges, illness behavior and illness.


Temporal uncertainty, anticipation time, and cognitive coping under threat. The effects of temporal uncertainty, anticipation time, and coping orientations on anticipatory reactions to an inevitable electric shock were investigated. One hundred eight male subjects were randomly assigned to the experimental conditions: they knew or did not know (temporal uncertainty) exactly when shock would occur; they waited either 1, 3, or 12 minutes for the shock, and were given either a vigilance, avoidance, or no orientation for preparing for the shock. Physiological measures were recorded continuously and pre- and post-trial questionnaires were completed to assess thoughts and feelings. Temporal uncertainty and anticipation time interacted to affect stress reactivity--i.e., as shock became imminent, temporal uncertainty led to the increased use of avoidant (as opposed to vigilant) modes of coping and was associated with a progressive lowering of reactivity, especially under the longer anticipation times. The results are consistent with the theory that coping processes mediate stress reactions under conditions of threat.


Urban commuting: crowdedness and catecholamine excretion. Male passengers regularly commuting by train on the Stockholm-Nynäshamn line were investigated on two morning trips to Stockholm. These trips were made under different levels of crowding, before (Trip 1) and after (Trip 2) a period of gas rationing during the oil crisis in 1974. However, seats were available for almost everyone during both trips. One group of subjects boarded the train at its first stop (Nynäshamn), the other midway on its route (Västerhaninge). Physiological reactions were assessed from the rate of catecholamine excretion in urine and subjective experiences were measured by self-ratings. The results showed that feelings of discomfort grew more intense as the train approached Stockholm and the number of passengers increased. Perceived crowdedness increased as the square of the number of passengers. During both trips the subjects from Nynäshamn (longer trip) had a lower rate of adrenaline and noradrenaline excretion on the train than those from Västerhaninge. Furthermore, it was found that the rate of adrenaline excretion was higher for both groups during Trip 2, when the train was more crowded. The results support previous findings indicating that the stress involved in travelling by train varies more with the social and ecological conditions of the trip than with its length or duration.


Underload and overload in working life: outline of a multidisciplinary approach. A research project is outlined in which concepts and methods from social psychology and psychophysiology are integrated in the study of human adaptation to underload and overload related to technically advanced work processes. Attempts are made to identify aversive factors in the work process by studying acute stress reactions, e.g., catecholamine excretion, in the course of work and relating these to long term, negative effects on well-being, job satisfaction and health. Data from a pilot study of sawmill workers support the view that machine-paced work characterized by a short work cycle and lack of control over the work process constitutes a threat to health and well being.


Surgery and mortality in peptic perforation.


Life events before myocardial infarction. One-hundred and-twenty-one men between the ages of 35 and 65 who had been admitted to a Coronary Care Unit were interviewed within a mean of 2.3 days of that admission, concerning their experience of certain specified predesignated life events in the previous three months before myocardial infarction. Ninety-one of them who were proven to have sustained a myocardial infarction were randomly matched individually with 91 men from an industrial payroll for sex, age and occupational level. Both groups matched, as groups, on marital status and household size. Significantly more patients (p less than .01) than comparison subjects reported these life events in the three weeks before infarction, whether this was experienced acutely or in an anginal setting. Most events were apparently independent of patients' or comparison subjects control; these were reported significantly more often by patients, both during the entire 12 weeks prior to illness (p less than .01) and during the three weeks immediately before infarction (p less than .02). Methodological difficulties are delineated which hinder credence as to the role of life events before illness.


Effects of prolonged stress on coping style in terminal renal failure patients. Fifty-nine patients with terminal renal failure and 59 comparison subjects matched on age, sex, origin, education and marital status were administered the Shanan Sentence Completion Test at the onset of hemodialysis, to test the hypothesis that 1) prolonged stress reduces the tendency to cope actively and that 2) the extent of reduction would vary according to the patients' background. Findings provided massive support for the first hypothesis; in nearly all aspects of coping style investigated, hemodialysis patients obtained significantly lower scores, indicative of passivity, negative self-perception and of tendencies to withdraw by denial from the harsh reality. Findings on the second hypothesis were equivocal. Only sex and education showed interaction with illness and coping. Women appeared to be more vulnerable than men. The effects of education on coping were reduced as a consequence of the illness. Findings are discussed in terms of their implications for stress research and for the advancement of preventive measures in hemodialysis treatment.


Giant cell tumour of the bone.

Giant cell tumour of the bone.


Filarial acute abdomen in pregnancy.

Filarial acute abdomen in pregnancy.


Effect of PGF 2 alpha and stress on the ascorbic acid content of rat adrenal gland (a histochemical observation).


Dubin-Johnson syndrome (Dubin-Johnson-Sprinze disease).


Surgery and mortality of perforated peptic ulcer.


Leucorrhoea in perimenopausal women.

Leucorrhoea in perimenopausal women.


Pulmonary stenosis.

Pulmonary stenosis.


Percutaneous needle biopsy of lung (new dimensions).


Profile of central and peripheral nervous system involvement in muscular dystrophy.


Under-Five's Clinic and rural India.

Under-Five's Clinic and rural India.


Rheumatic heart disease in Pondicherry (a clinical study).


Comparative trial of co-trimoxazole and tetracycline in the treatment of lower respiratory tract infections.


New approaches to the delivery of child care.


Health care of rural population of 0-5 age group.


Small-for-dates babies (follow-up study of 107 cases).


Acute intestinal obstruction (a review of 1020 operated cases).


Panhypopituitarism presumably of diabetic origin.


Some common skin conditions of the newborn.


Profile of rheumatic heart diseases in India.


Primary nasal neuroblastoma.

Primary nasal neuroblastoma.


Endomyocardial fibrosis.

Endomyocardial fibrosis.


Peritonitis in children.

Peritonitis in children.


Anodontia.

Anodontia.


Origin of popliteus muscle in man.

Origin of popliteus muscle in man.


Plasma non-esterified fatty acids in diabetes mellitus with coronary artery disease.


Changing concepts of medical education.

Changing concepts of medical education.


Paediatric premedication with oral atropine.


Role of general practitioners in medical education.


Vogt-Koyanagi syndrome.

Vogt-Koyanagi syndrome.


Recommendations of the II National Conference on Medical Education, March 1976 (under the auspices of the Indian Medical Association).


Orthopaedic training in developing countries.


Barefoot doctors or health auxiliaries: a plan of action.


Tuberculous infection in New Orleans.

Tuberculous infection in New Orleans.


Stevens-Johnson syndrome: a pediatric experience.


Fiberoptic bronchoscopy: experience with 100 consecutive patients.


Heart throbs: fact or artifact?

Heart throbs: fact or artifact?


Hypersensitivity in the anterior median eye of a jumping spider. Changes in sensitivity of the photoreceptor cells of the anterior median eye of the jumping spider Menemerus confusus Boes. et Str. have been studied by recording electroretinograms (ERGs) and receptor potentials. The amplitudes of the responses (ERGs and receptor potentials) increase during repetitive stimulation, with a maximum increase at 3-5 s intervals. The sensitivity of the photoreceptor cell is greater for about 60 s following illumination (maximum magnitude at 3-5 s) than it is during complete dark adaptation. This phenomenon, which we call 'hypersensitivity', is lost within one day following surgery in physiological saline. Upon loss of hypersensitivity, the sensitivity decrease during light adaptation is greater than for the normal eye and the small increase of sensitivity following the onset of illumination observed for the normal eye is lost.


Spirillum swimming: theory and observations of propulsion by the flagellar bundle. The hydrodynamics and energetics of helical swimming by the bacterium Spirillum sp. is analysed using observations from medium speed cine photomicrography and theory. The photographic records show that the swimming organism's flagellar bundles beat in a helical fashion just as other bacterial flagella do. The data are analysed according to the rotational resistive theory of Chwang & Wu (1971) in a simple-to-use parametric form with the viscous coefficients Cs and Cn calculated according to the method of Lighthill (1975). Results of the analysis show that Spirillum dissipated biochemical energy in performing work against fluid resistance to motion at an average rate of about 6 X 10(-8) dyne cm s-1 with some 62-72% of the power dissipation due to the non-contractile body. These relationships yield a relatively low hydromechanical efficiency which is reflected in swimming speeds much smaller than a representative eukaryote. In addition the Cn/Cs ratio for the body is shown to lie in the range 0-86-1-51 and that for the flagellar bundle in the range 1-46-1-63. The implications of the power calculations for the Berg & Anderson (1973) rotating shaft model are discussed and it is shown that a rotational resistive theory analysis predicts a 5-cross bridge M ring for each flagellum of Spirillum.


The effects of osmotic stress on the electrical properties of the axons of a marine osmoconformer (Mala squinado. brachyura: crustacea). In contrast to the depolarization observed in hyperosmotic media, exposure of peripheral nerve to hyposmotic conditions induced pronounced axonal hyperpolarization. It is suggested that this hyperpolarization resulted from increased potassium and chloride permeabilities which could assist axonal volume regulation in hyposmotic conditions. The hyperpolarization was readily reversible, but the spike-generating mechanism suffered irreversible damage at hyposmotic concentrations below 665 m-osmoles. It is suggested that this axonal damage contributes to the lethal effects of hyposmotic stress in this crustacean osmoconformer and, possibly, in some euryhaline osmoregulators.


Passive exchanges during water vapour absorption in mealworms (Tenebrio molitor): a new approach to studying the phenomenon. The weights of single mealworms were continuously recorded at 20 degrees C during exposure to periods of constant humidity and to abrupt changes in atmospheric vapour pressure. Two exchange stages were recognized in each animal. Weight changes were either limited to slow losses, suggesting transpiration through the external cuticle, or showed more rapid humidity-dependent gains as well as losses. Rapid exchanges indicated that water was gained or lost through permeable barriers, from a fluid compartmet of significantly lower vapour pressure than the haemolymph, equivalent to about 90% R.H. Weight gains and losses during humidity changes provided evidence of a significant, passively exchanging fluid compartment located between the exchange surface and absorbing mechanism. Weight changes in faecal pellets following their elimination provide further support for a rectal site of atmospheric absorption.


Mediastinal tumors. IV. Malignant thymoma.


Neuronal basis of a sensory analyser, the acridid movement detector system. III. Control of response amplitude by tonic lateral inhibition. 1. The Lobular Giant Movement Detector neurone (LGMD) of Schistocerca responds with spikes when small areas of the visual field change in luminance. Previous work has shown that changes of +/- 1 log 10 unit are enough to produce maximal ON and OFF responses. 2. Using a 5 degree test area, it is shown that the number of spikes generated by such a stimulus depends on the luminance of the surrounding area. When the surround is dark, the response is maximal; when it is brightly lit, the response is minimal. Intermediate intensities produce intermediate values of response. A X 2 change in response is produced by about 3 log 10 units change in surround intensity. 3. A bright annulus, with diameters of 10-5 degrees and 25-8 degrees, inhibits both ON and OFF responses when concentric with the 5 degree test area, but not when it is 30 degrees eccentric to the test area. The inhibitory effect shows no decrease after 4 min. 4. These results are interpreted to indicate a tonic lateral inhibitory network, sited peripherally in the optic lobe prior to the divergence of the separate ON and OFF channels found in the projection from the medulla to the LGMD. It is probably identical with that described for the lamina by previous workers.


Primitive nervous systems: electrophysiology of the pharynx of the polyclad flatworm, Enchiridium punctatum. 1. Electrical activity accompanying motor activity can be recorded from the excised pharynx of Enchiridium punctatum. Multiple stimuli elicit behaviour which consists of an initial aperture closure followed by extension and then peristalsis. If the stimulus parameters are increased the preparation bends from side to side instead of proceeding through the behavioural sequence. Bending appears to inhibit other movements differentially. 2. The conduction involved with peristalsis is polarized and proceeds in a proximal direction. 3. With stimulus intensities greater than those needed to produce the behavioural response an initial muscle potential (IMP) is evoked. The IMP is frequency sensitive. Maximum facilitation occurs within 100 ms and drops to 50% of maximum within 250 ms. 4. Conduction velocities of the IMP range from 0-05 m s-1 to 1-9 m s-1. Conduction velocities appear to increase with facilitation.


Heart action of the freshwater bivalve Anodonta anatina during activity. 1. Heart action of Anodonta anatina (L.) was investigated by recording the electrocardiogram (ECG), heart impedance, and ventricular and pericardial cavity pressure during different aspects of the normal behaviour. The contribution of mechanical and nervous mechanisms in controlling changes in heart action is discussed. 2. Pressure recordings were generally more reliable than the other methods and it is suggested that pericardial pressure pulses indicate the stroke volume output of the ventricle. 3. During spontaneous periods of prolonged shell closure there was an initial small increase in heart activity followed by a large reduction in both heart rate and systolic pressure, indicating that total heart output was considerably reduced. When the shell reopened, heart rate increased very rapidly with an initial overshoot of the normal level; systolic pressure increased more slowly with no overshoot. 4. These major changes in heart activity appear to be associated with respiratory changes and are controlled largely by the nervous regulatory system, but some minor rhythmic variations in the amplitude of heart beat are probably caused by mechanical factors. 5. Characteristic patterns of change in heart action were recorded during burrowing. These appear to result from haemodynamic changes associated with the muscular movements of the digging cycle. Control of the heart by the nervous regulatory system is apparently of much greater importance in relation to respiratory control than in relation to the haemodynamic functioning of the fluid-muscle system in locomotion.


Rhythmic swimming activity in neurones of the isolated nerve cord of the leech. 1. Repeating bursts of motor neurone impulses have been recorded from the nerves of completely isolated nerve cords of the medicinal leech. The salient features of this burst rhythm are similar to those obtained in the semi-intact preparation during swimming. Hence the basic swimming rhythm is generated by a central oscillator. 2. Quantitative comparisons between the impulse patterns obtained from the isolated nerve cord and those obtained from a semi-intact preparation show that the variation in both dorsal to ventral motor neurone phasing and burst duration with swim cycle period differ in these two preparations. 3. The increase of intersegmental delay with period, which is a prominent feature of swimming behaviour of the intact animal, is not seen in either the semi-intact or isolated cord preparations. 4. In the semi-intact preparation, stretching the body wall or depolarizing an inhibitory motor neurone changes the burst duration of excitatory motor neurones in the same segment. In the isolated nerve cord, these manipulations also change the period of the swim cycle in the entire cord. 5. These comparisons suggest that sensory input stabilizes the centrally generated swimming rhythm, determines the phasing of the bursts of impulses from dorsal and ventral motor neurones, and matches the intersegmental delay to the cycle period so as to maintain a constant body shape at all rates of swimming.


Modulation of Hydra attenuata rhythmic activity: phase response curve. We investigated the effect of photic stimulation on the frequency of Hydra attenuata column contractions. We used positive or negative abrupt light transitions, single or repetitive light or darkness pulses, and alternation of light and darkness periods. The main results are: (a) The frequency of the contraction pulse trains (CPTs) varies transiently in response to an abrupt variation of the light intensity. (b) CPTs in progress can be inhibited by different types of photic stimuli. (c) The response time to a single photic stimulus varies during the inter-CPT interval and depends also on the polarity of the stimulus. (d) The CPTs are entrainable with repetitive light stimulation of various frequencies. (e) Long-lasting variations of the frequency of CPTs occur after the end of a repetitive light stimulation. We suggest that the mechanism responsible for the rhythym of column contractions is quite similar to that on which other biological rhythmic phenomena are based.


Evidence for calcium inactivation during hormone release in the rat neurohypophysis. 1. A study has been made of the relationship between 45Ca uptake into and hormone release from isolated rat neurohypophyses incubated in vitro. 2. Hormone secretion is triggered by high-K (56 mM) but long exposure to the stimulus does not generate a maintained release of hormone. 3. When hormone release began to wane, addition of Ba of La increased hormone output which suggests that the decline in output did not result from depletion of the neurosecretory granules at the nerve terminals. 4. 45Ca uptake is enhanced in the presence of high-K concentration, but the initial high rate declines during long exposure to the potassium stimulus with a time constant similar to that of the decline in hormone release. 5. After a period of incubation in a K-rich, calcium-free medium, addition of calcium to the medium induced hormone release. The magnitude of this release was dependent on the time of exposure to excess potassium. 6. After inactivation of secretion, mobilization of internal calcium by means of a calcium ionophore increased hormone release.


Events following the infections of enucleate cells with measles virus. The development of measles virus (Edmonston) and SSPE measles virus (Horta-Barbosa) has been examined in enucleate BSC 1 cells. New antigen synthesis in measles virus infected enucleate cells has been demonstrated by fluorescent antibody, by the formation of extensive syncytia from enucleate cells alone and by analysis of polypeptide formation by polyacrylamide gel electrophoresis. All polypeptides formed in nucleate cells were also present in enucleate cells but the amount synthesized was reduced to around 20% of that in nucleate cells. There was also a significant reduction in the amount of antigen detected by fluorescent antibody in enucleate as compared to nucleate preparations. Examination of RNA synthesis in infected enucleate cells revealed only a marginal increase in acid-insoluble material. Titration of the output of infectious virus from enucleate cells infected at both 37 and 31 degrees C indicated a consistent reduction of almost two log units compared to nucleate cells. That the enucleate cells were capable of replicating input genome at these times was demonstrated by the successful growth of respiratory syncytial virus, both at 37 and 31 degrees C. SSPE measles virus grew to higher yield in nucleate BSC 1 than measles virus but there was again a reduction of more than two log units in enucleate cells. All polypeptides synthesized in SSPE infected nucleate cells were apparent in enucleate cells.


Homogenization-resistant and -susceptible components of tobacco mosaic virus replicative form RNA. When prepared from tissue frozen with liquid nitrogen, tobacco mosaic virus replicative form RNA (TMV RF) was uniform in size but when prepared by high-speed homogenization, or when TMV RF prepared with liquid nitrogen was homogenized, 80 to 90% of the RF broke into relatively discrete pieces. The unbroken RF was not fragmented by additional homogenization. The TMV RF components susceptible and resistant to breakage, respectively, were synthesized with similar kinetics in relation to length of labelling period, but the slightly more resistant component was synthesized during the early infection period. Both components were produced by different strains of TMV but leaves infected with cowpea chlorotic mottle or southern bean mosaic viruses yielded only RF resistant to breakage. TMV replicative intermediate RNA was also broken by homogenization. The occurrence of the two RF components may be of significance in the replication of RNA viruses.


Ortho- and paramyxoviruses from migrating feral ducks: characterization of a new group of influenza A viruses. Ortho- and parainfluenza viruses isolated from the cloacas of migrating feral ducks shot on the Mississippi flyway included three strains of influenza. A virus (Hav6 Nav1, Hav6 Nl, Hav7 Neq2) as well as Newcastle disease virus. One influenza virus, A/duck/Memphis/546/74, possessed Hav3 haemagglutinin, but the neuraminidase was not inhibited by any of the known influenza reference antisera. The neuraminidase on this virus was related to the neuraminidases on A/duck/GDR/72 (H2 N?), A/turkey/Ontario/7732/66 (Hav 5 N?), A/duck/Ukraine/1/60 (Hav3 N?) and A/turkey/Wisconsin/68. We therefore propose that the neuraminidase on this group of influenza viruses be designated Nav6. The A/duck/Memphis/546/74 influenza virus caused an ocular discharge in 1 of 5 ducks and was shed in faeces for 10 days; it was stable in faecal samples for up to 3 days at 20 degrees C. These results suggest that ecological studies on influenza in avian species should include attempts to isolate virus from faeces. Faecal-oral transmission is an attractive explanation for the spread of influenza virus from feral birds to other animals.


Xf phage invading the host cells with their protein coats. The Xf phage coat protein associated with infected cells could not be removed by washing with antiserum and tris-EDTA buffer. Although the infected cells were consecutively washed 6 times with tris-EDTA buffer, the ratios of parental phage 6H-DNA to 14C-protein were not changed. A considerable amount of the parental 14C-protein and 3H-DNA in the original ratio were detected in the membrane and the soluble cytoplasmic fractions of infected cells. The studies of the change in Xf 14C-protein and 3H-DNA incorporation into the host cells and their release showed that DNA and protein penetrate together into the host cells during the first 60 min after infection (p.i.). While virtually all parental DNA was conserved, re-utilized and released from the infected cell 60 min p.i., no apparent release of parental protein was observed. Approx. 40% of the parental protein became degraded and could be washed from the infected cell after 90 min; the rest of the parental protein remained and probably was re-utilized by the host.


On the regulation of protein synthesis in vaccinia virus infected cells. All eukaryotic mRNA species show a characteristic individual translational efficiency under conditions of restricted polypeptide chain initiation caused by an increase in the osmolarity of the growth medium. In vaccinia virus infected L cells or HeLa cells virus mRNAs can be grouped into classes on the basis of their relative labelling under standard and hypertonic conditions. Under the latter conditions, most of the "early" mRNAs possess very high translational efficiencies, most of the "intermediate" mRNAs show an intermediate efficiency and the most prominent "late" mRNAs show a translational efficiency which is lower than that of other virus mRNAs but still higher than the average cellular mRNA. Late in the infection cycle virus mRNAs with a relative low translational efficiency are preferentially translated under standard growth conditions whereas "early" virus mRNAs which are still present and which show a higher translational resistance to hypertonic conditions are not translated. These results indicate a unique translational control operating late in the growth cycle of vaccinia virus.


A rapid method for the quantitative study of RNA from canine distemper virus infected cells. Centrifugation through CsCl was used to isolate 32P-labelled RNA in a one-step purification procedure. The method is suitable for quantitative as well as preparative studies and appears to have considerable advantages over conventional methods of RNA extraction. We have used this procedure to investigate the RNA synthesized in Vero cells infected with canine distemper virus (CDV). We show that the combination of CsCl centrifugation and affinity chromatography on poly-U Sepharose provides a rapid method for isolating messenger RNA from virus infected cells.


The use of contact B mode ultrasound in pediatric ophthalmology. It has been shown that an ultrasound system such as the Bronson-Turner Contact B Mode can be added, fruitfully, to the armamentarium of the individual pediatric ophthalmologist. It can be used quickly and precisely with an ophthalmological training and without patient discomfort or anesthesia no matter what the age. Valuable anterior segment information can be readily gained without a waterbath. Examples are shown of children with opaque media who were found to have congenital cataracts and congenital aphakia, microphthalmos, PHPV, retinal anomalies and ectatic coloboma.


Inhibition of vaccinia virus growth by the nucleoside analogue 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide (virazole, ribavirin). Virazole or Ribavirin (1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide) inhibits the growth of vaccinia virus at a concentration ode to a certain extent in the presence of Virazole, the DNA fails to acquire resistance to deoxyribonuclease and virus particles are not formed. Reversibility of the antiviral effect occurs when the drug is washed out from the infected cultures or when guanosine at an equimolar concentration is added.


Fascia lata sling in blepharoptosis: enhancement of result by postoperative adjustment.


Rhinosporidiosis in children.

Rhinosporidiosis in children.


Freeze-cleaved replicas of the anterior surface of the vitreous body in premature infants. An electronmicroscopic structure of the anterior vitreous surface of premature human infant cadavers at the age of six, seven and eight months demonstrated with replica technique is presented. A regular hexagonal structure has been found behind the center of the lens. Towards the equator of the lens this structure is losing its regularity and may exhibit the forms of pentagons, rectangles, etc. This is probably due to the technique applied in the preparation. In one case a "mixed type" of fibrillar structure appeared, the arrangement of which does not seem to fit into the present concept of the orientation of the collagen fibrils.


The fusional balancing innervation in heterophoria for control of antipodal deviations at a null point: a previously undescribed type of binocular innervation.


Strabismus in children with cerebral palsy. A group of 238 patients with cerebral palsy were identified of whom 108 could be studied through their charts. Only 20 of these patients had strabismus. Of this group, 10 received no surgical therapy. In these patients followed up to a period of four years, no evidence of significant change in the strabismic deviation was found. Patients who were treated by medical and optical means alone did not show evidence of improvement. Surgical therapy was effective in providing a cosmetically acceptable result. Surgery was performed between two and one-half and 13 years of age in eight patients, with an average age of surgery of 6.5 years. The results which we obtained are comparable to those obtained by others at an earlier age. It does not appear that the age of surgery affects the ultimate cosmetic nor functional outcome in children with cerebral palsy.


Infantile cataracts, Coats' disease, and ketotic hypoglycemia.


Optic disc pigmentation associated with a field defect following chickenpox.


Aplasia of the optic nerve.

Aplasia of the optic nerve. A case of unilateral true aplasia of the optic nerve in an otherwise normal and healthy child is presented. Twenty-eight previously reported cases of aplasia have been reviewed and classified according to criteria which we have presented. Only six of these cases, including the present case, represent true aplasia, manifested by total blindness and complete absence of the optic disc and retinal vessels.


Recurrence risks for retinoblastoma: a model for autosomal dominant disorders with complex inheritance. The characteristics of retinoblastoma pertinent to genetic counseling are presented. Vogel's model for the inheritance pattern of the tumor is described and then used to derive recurrence risks for the important genetic counseling situations in retinoblastoma. The effect on the risk of the degree and number of unaffected relatives to the index case in a pedigree is quantified.


Comparative study of the surgical management of congenital esotropia of 50 prism diopter or less. Fort-nine patients with congenital esotropia of 50 prism diopters or less were selected at random. Twenty-five had bimedial recessions performed initially followed by bilateral rectus resections when needed. Twenty-four had recession/resections performed initially and when needed, were followed by the same procedure on the fellow eye. The average corrections obtained after first procedure and after second procedure were compared and analyzed. The results showed the initial procedures of both groups to be equally effective. The results obtained after the second procedure in the recession/resection group were significantly better. Therefore, it would appear that for the patient we have described, the most effective surgical approach is ultimately the surgical regimen of recession/resection followed by the same procedure on the fellow eye.


Thrombosis of the central retinal artery in retinoblastoma.


Clinical picture and management of subperiosteal hematoma of the orbit. A subperiosteal hematoma was seen in a 14-year-old boy following a blow to his head during a car accident. The involved orbit exhibited exophthalmus and inability of the eye to move above the horizontal. X-rays revealed a hairline fracture of the skull and a hemotympanum was found on the injured side. A subperiosteal hematoma of the orbital roof was suspected. Needle aspiration of the blood from the orbital hematoma resulted in an almost immediate cure of all orbital and occular problems.


A new approach to the Hirschberg test.

A new approach to the Hirschberg test. Forty patients with horizontal strabismus were examined in order to establish a correlation between measurements obtained by the alternate cover under the amblyoscope and measurements made by passively placing the corneal reflex in symmetry of that of the fixating eye. In order to reduce errors, the pupils were constricted to two mms and a very intense and bright one mm corneal reflex produced. Both measurements were very similar and a correlation of r=0,94 was found. Accuracy was significant at the p=0,005 level.


Juvenile glaucoma in the Rubinstein-Taybi syndrome. A 10-year-old retarded child was seen by an ophthalmologist because of strabismus. Examination of the optic nerve heads revealed cupping consistant with glaucoma and initiated a referral. The appearance of this girl, with flat-broad based thumbs and toes, small head, low set ears, high arched brows, antimongoloid slant to the eyes, high arched palate, associated with mental retardation, and strabismus suggested the Rubinstein-Tabyi Syndrome. Gonioscopy revealed a high iris insertion, while tonometry indicated mildly elevated pressures in the right eye. Examination of the optic nerve heads showed large glaucomatous type cups, more so on the right with compromise of the temporal rim. Trabeculectomy was effective in controlling the intraocular pressure in the right eye. The association of juvenile glaucoma with the Rubinstein-Taybi Syndrome requires that ophthalmic referral to assess glaucoma be an essential part of the evaluation.


Hyphema in infancy and childhood: a brief review and report of an unusual case.


Trabeculectomy for adolescent onset glaucoma in the Sturge-Weber syndrome. Two patients with adolescent onset glaucoma associated with Sturge-Weber Syndrome have been presented. Both patients were successfully treated with ab externo trabeculectomy. This procedure is suggested as an additional means for controlling the late onset glaucoma associated with the syndrome without provoking additional complications or deterioration. Early treatment is emphasized before the irreversible changes of chronic glaucoma become manifest.


Feathered eyebrows and high myopia.

Feathered eyebrows and high myopia.


Enucleations in Nigerian Igbo children.

Enucleations in Nigerian Igbo children. A series of 32 eyes enucleated from Nigerian Igbo children has been reviewed. Retinoblastoma accounted for 19 enucleations, both sexes being equally affected and most growth being far advance. The seven cases of known trauma were with one exception encountered in boys and involved mostly plant material. Inflammatory pseudotumor was seen in two girls during the first year of life and malignant lymphoma in a boy of 15 years. Corneal ulceration was responsible for enucleation in three boys, one of them being the aftermath of measles.


Experimental creeping muscle.

Experimental creeping muscle. Using healthy rabbits, medial and lateral rectus muscles were recessed. Classical Swann incisions, along with limbal incisions with and without conjunctival recession, were performed. The second variable of reactability of suture material, utilizing absorbable 6-0 chromic and nonabsorbable 6-0 tycron sutures, was tested. Using a 9-0 nylon marking suture for documentation of area of recession, anterior advancement could be accurately measured. Five months after surgery, the animals were sacrificed, and each muscle was examined. Regardless of incision perfored and regardless of suture type used, no anterior advancement of retroplaced muscles occurred.


Pediatric dosing considerations in ophthalmology. In almost all cases concerned with topically applied ophthalmic drugs, no distinction is made between adults and infants or children with regard to the concentration and amount of drug used. Even though maximal therapeutic effects are usually observed, there can be significant short- and long-term toxicity problems. Based on some modest assumptions it should be possible to reduce doses without sacrificing therapeutic effectiveness in infants and children up to three years of age. The overriding concern should be, however, potential toxicity problems which may exist  in infants and children, in particular the neonate, due to their low body weight and the fact that in most cases, more than 90 percent of an instilled dose in potentially available to act systemically. Total body surface area can be used as a measure for determining potentially toxic doses in children. Especially for potent drugs, with both short- and long-term manifestations, toxicity considerations may necessitate some sacrifice in therapeutic effectiveness of a given drug or switching to an alternate drug in order to insure a reduction in toxic side effects.


Panuveitis -- presenting symptom of systemic sarcoidosis in a child.


Giant hairy nevus: preventable cause of amblyopia. An infant with a congenital giant hairy nevus causing occlusion of the visual axis of the right eye is presented. The nevus was removed at the early age of three weeks because of concern that the child would develop deprivation amblyopia. Since such nevi can undergo malignant changes, early removal may be justified for that reason alone.


The special blood supply of the retina in optic nerve gliomas.


Abnormal macular arteriovenous communication.


Electroretinogram and visually evoked cortical potential in Tay-Sachs disease: a report of two cases. The electroretinogram (ERG) and visually evoked cortical potential (VECP) were recorded in two cases of Tay-Sachs disease where diagnosis was well established: 1. ERGs of high amplitude and of normal wave form were recordable with prominent oscillatory potentials. Responses were always equal in both eyes. 2. VECPs were extinguished in both cases. These findings were interpreted as showing that Tay-Sachs disease is caused by the degeneration of ganglion cells in the nervous system and that the ganglion cells in the retina do not affect the ERGs of vertebrate animals.


Isolated oculomotor nerve palsy in childhood from leptomeningeal polymorphic cell sarcoma.


Ocular pathology of Lowe's syndrome in a female infant. The ocular pathologic findings are presented in a case of a female infant with clinical and biochemical evidence of Lowe's syndrome. A small but increasing number of reports suggest that the mode of inheritance is not always sex-linked. The ocular histopathologic findings in the present case parallel those previously described for this syndrome. While the pathologic findings in other systems are variable, it is thought that the constellation of lens and anterior segment changes are sufficiently characteristic to identify cases of Lowe's syndrome.


Atypical vertical retraction syndrome: a case study. A case of unilateral retraction of the eyeball in downward gaze as well as downshoot with the retraction when an outward horizontal movement of the contralateral eye was attempted has been introduced. The case has an exotropia with inability to adduct involved eye. The face turned to the left to minimize diplopia in the primary position. During the retraction, the lid widened slightly. An analyses by electrooculography and electromyography suggested that there was an abnormal neural connection between the vertical recti of the involved eye and the contralateral lateral rectus muscle. Co-contraction of vertical recti and loss of the reciprocal innervation were seen at the involved eye. Surgical treatment for exotropia, and total transplantation of vertical recti to the insertion of the involved medial rectus resulted in a slight reduction of the exotropia with the face straight, but not in the retraction as well as adduction and elevation of the involved eye. Based on the results, a central mechanism to produce above abnormality was described.


Bilateral retinoblastoma and verbal intelligence. Sighted and blind patients with bilateral retinoblastoma were compared with their unaffected siblings on standardized tests of verbal intelligence. IQs of blind subjects were found to be significantly higher than those of their siblings while no such difference was noted between sighted subjects and their controls. It was concluded that the IQ superiority of the blind group is associated with blindness rather than with the disease.


Embryopathic time-table of cataract formation in congenital rubella.


Ectopia lentis with Marfan's syndrome -- case with a useful optical correction.


Recurrent nonaccidentally inflicted chemical eye injuries to siblings.


Massive congenital ocular toxoplasmosis.


Familial retinoblastoma--a cautionary tale.


The cornea in mucolipidosis IV.

The cornea in mucolipidosis IV.


Physiological mediation of attitudinal responses. A study was undertaken to try to ascertain the best model of the relationship between stimuli, physiological responses, and attitudes. Subjects were asked to indicate their attitudes toward various nations after having received various bogus information about how they responded physiologically to the stimuli. The results indicate the following: (a) In general, attitudes appear to be physiologically mediated; (b) physiological mediation of attitudes is strongest when prior knowledge about the stimuli is low but is independent of prior attitudes about the stimuli; (c) bogus physiological feedback does not seem to be verbally mediated; and (d) the most fruitful distinction between types of bogus feedback is between any feedback versus no feedback, not between change versus no change or between increase versus decrease in response.


Sex typing and the avoidance of cross-sex behavior. This article presents evidence for the hypothesis that cross-sex behavior is motivationally problematic for sex-typed individuals and that they actively avoid it as a result. In particular, when asked to indicate which of a series of paired activities they would prefer to perform for pay while being photographed, sex-typed subjects were more likely than either androgynous or sex-reversed subjects to prefer sex-appropriate activity and to resist sex-inappropriate activity, even though such choices cost them money. Moreover, actually engaging in cross-sex behavior caused sex-typed subjects to report greater psychological discomfort and more negative feelings about themselves.


Retinoblastoma in an eighteen-year-old male.


Clinical applications of research in strabismus.


Perforating injuries of the eye in childhood.


Sugars and sugar derivatives which inhibit the short-circuit current of the everted small intestine of the rat. 1. The short-circuit current of everted rat intestine supported on a perforated cannula proved to be stable for up to 3 hr and has been used to study competition between transportable and non-transportable sugars. 2. 4,6-O-Ethylidene-alpha-D-glucopyranose (ethylidene glucose) and 4,6-O-benzylidene-e alpha-D-glucopyranos (benzylinene glucose), two nontransportable inhibitors of the hexose transfer system in human erythrocytes, were found to reduce the short-circuit current generated by transportable sugars such as galactose or 3-O-methyl glucose. 3. These compounds were also found to reduce the basal short-circuit current established by the everted intestine in a sugar-free Krebs solution. Both types of inhibition approached saturation at the higher concentrations used. 4. Similar inhibitory properties were shown by mannose, a non-actively accumulated monosaccharide, and by the beta-disaccharides lactose and cellobiose. 5. It is suggested that this common pattern of behaviour is due to the ability of these compounds to react with the sites for active hexose transfer but without translocation by the system. The significance of the inhibition of the basal short-circuit current is briefly discussed in this context.


Space occupying orbital lesions in Indian children: a clinical study of 66 consecutive cases.


The surgery and results following traumatic cataracts in children. Ninety eyes undergoing surgery for the removal of traumatic cataracts have been evaluated. In this group, the visual results relate to the coincident complications and amblyopia. Most commonly, they may be related to the extent of the preoperative trauma to the injured eye or to the age of the patient at the time of the injury. Forty-eight eyes (54%) achieved an acuity of 20/20 to 20/80 with 38 (42%) eyes achieving acuity of 20/20 to 20/30. A visual result of 20/80 or better was obtained in 12 eyes of the 29 patients six years of age or less.


Afferent sympathetic nerve fibres with aortic endings. 1. We recorded the electrical impulse activity of thirty-five single afferent fibres with aortic endings isolated from the third to the sixth left thoracic sympathetic rami communicantes of anaesthetized cats. The endings of each fibre were localized by mechanical probing of the opened aorta at the end of each experiment. 2. Twenty-four fibres had a single aortic receptive field. Eleven fibres had several and distinct receptive fields (from two to four): they were usually located in nearby aortic areas or, in addition, in other proximal portions of the arterial tree or in the adjacent pleura and connective tissue. 3. Twenty-nine fibres had conduction velocities ranging between 5 and 27 m/sec (Group Adelta), while six fibres had conduction velocities between 0-2 and 1-2m/sec (Group C). 4. The spontaneous impulse activity was in phase with the aortic pressure pulse and consisted of not more than one impulse per pressure pulse. It was increased during increases in aortic pressure and, conversely, decreased during decreases in aortic pressure. In vivo and post mortem studies showed that these mechanoreceptors had an impulse activity which rapidly adapted during sustained stimuli. They thus seem to signal pulsatile aortic stretch. 5. These aortic sympathetic afferents are likely to be part of a nervous pathway through which pressor reflexes, exhibiting positive feed-back characteristics, can elicited.


Manganese-dependent propagated action potentials and their depression by electrical stimulation in guinea-pig myocardium perfused by sodium-free media. 1. Propagated action potentials were recorded in right ventricular papillary muscles from guinea-pig heart while exposed to Na-free, Ca-free and Mg-free solutions containing Mn. 2. When Na was totally replaced by 95 mM-Mn the overshoot was about 45 mV while the resting potential was about -90mV. 3. The overshoot of action potentials was increased by about 20-30 mV per tenfold increase of Mn concentration over the range of 2-50 mM. 4. Similar increases of overshoots with increasing of Mn concentration also occurred in the presence of 0-6 mM-Ca. Increasing of Ca from 5 to 20 mM had little influence on the overshoot but shortened the duration of the Mn-dependent action potential in the presence of 5 mM-MN. 5 Mn-dependent action potentials were not depressed by 3 X 10(5) M tetrodotoxin but by La. 6. These results suggest that Mn passes through the slow inward current channel to generate the action potential seen under the Na-free condition. 7. The overshoot and duration of the Mn-dependent action potential decreased with stimulation. At stimulus frequencies (Hz) of 0-5, 0-2, 0-1, 0-017 and 0-0033 the overshoot of action potential in 5 mM-Mn Tyrode decreased by 0-5-1 mV per an action potential.  This depression of the action potential is explained by assuming intracellular accumulation of Mn.