Effect on carbohydrate metabolism of hypotensive adrenergic neurone blocking agents. In subjects with normal metabolism sugar tolerance was not appreciable influenced by 4 or 5-day courses of guanethidine and guanazodine. Upon perfusion in isolated rat liver, glycogen metabolism and hepatic sugar release were increased by guanazodine, guanethidine and debrisoquine alike. However, when these drugs were used simultaneously with chlorpropamide debrisoquine proved to be the weakest in counteracting the inhibitory effect of chlorpropamide on glycogen metabolism and sugar release. The observation may have clinical significance.


Myocardial lesions subsequent to roentgenotherapy. The myocardium was studied for changes caused by therapeutical X-ray irradiation in a necropsy material of 14 cases. The lesions found in the myocardial vessels, in the parenchyma and in the interstitium are described in detail. While the severity of the vascular changes was independent of the radiation dose, the gravity of parenchymal lesions was closely related to the dose employed.


Renal haemodynamics in mercuric chloride-poisoned dogs. Intrarenal haemodynamics and glomerular dynamics have been investigated in dogs with mercuric chloride-induced acute renal failure. The early phase (12 and 24 hr after the intravenous injection of 2 mg/kg HgCl2) is characterized by some decrease in renal bloodflow, due to afferent (preglomerular) vasoconstriction, while in the later phase (48 and 72 hr after poisoning a pronounced increase in RBF due to efferent (postglomerular) vasodilatation supervenes. Urinary volumes amounts to normal in the early phase, followed by oligo-anuria in the 48 and 72 hr groups. Extraction ratio of inulin, i.e. the filtration fraction and, consequently, glomerular filtration rate show a progessive decline and trend towards zero in the 72 hr group. The pronounced decrease in glomerular capillary pressure is due to both absolute and relative increase and decrease in afferent and efferent resistances, repectively. The progressive fall in effective filtration pressure is brought about by the decrease of glomerular capillary pressure, combined with increased colloid osmotic pressure due to haemoconcentration. Total and segmental intrarenal vascular resistances are reduced by hyperosmotic mannitol infusions resulting in renal hyperaemia. Glomerular filtration rate is, however, not influenced by mannitol and effective filtration pressure remains unaltered. Osmotic diuresis ensues in the early phase but urinary output is not restored in the late, i.e. oligo-anuric, phase.


Effect of carotid occulsion on intrarenal haemodynamics. Renal cortical medullary autoregulation was investigated by Sapirstein's 42K and 86Rb isotope fractionation methods under pentobarbital anaesthesia in nondiuretic dogs under normal conditions and during carotid occlusion. Cortical circulation appeared to be autoregulated in both conditions while medullary blood flow showed moderate autoregulation in the control as well as during carotid occlusion. In contrast to expectation, medullary blood flow was not increased by the blood pressure elevation following carotid occlusion but was reduced as a consequence of the simultaneously evoked reflectory vasoconstriction. Carotid occlusion resulted in a more pronounced enhancement of vascular resistance in the medulla than in the cortex. It was concluded that non-uniform intrarenal distribution of the autoregulatory as well as the reflectory vasoconstriction left pressure and filtration rate essentially unchanged in the cortical glomeruli but increased both in the iuxtamedullary ones. Carotid-occlusion diuresis, therefore, cannot be explained by increased medullary blood flow but may be result of augmented flow rate of tubular fluid through the loops of Henle.


Effect of alpha-pinene on the mixed function microsomal oxidase system in the rat. The microsomal fraction of the liver was studied for protein and cytochrome P 450 contents as well as for aminopyrine N-demethylase and aniline hydroxylase activity and for its butylisocyanide spectrum under the effect of alpha-pinene treatment. A moderate increase was found in the amount of microsomal protein and cytochrome P 450, a marked increase in the metabolism of both aminopyrine (substrate of type I) and aniline (substrate of type II), together with an increase in the butylisocyanide-absorption maxima of both haemoproteins involved in the reaction. Thus, alpha-pinene is a broad-spectrum enzyme inductor of the phenobarbital type, with certain quantitative differences.


The structure of gastrointestinal hormones. The primary and secondary structure of the three kinds of gastrointestinal hormones, i.e. gastrin, cholecystokinin-pancreozymin and secretin are discussed.


Gastrointestinal peptide hormone analogues. All derivatives of the C-terminal tetrapeptide fragment of gastrins with 17 amino acids elicit every physiological effect of the gastrin. This recognation led to detailed studies on the structure-activity relationships. In the case of cholecystokinin-pancreozymin, a substance consisting of 33 amino acids, the smallest sequence which possesses biological activity is the C-terminal octapeptide fragment. Synthesis of analogous derivatives of this fragment allowed the determination of the structural elements necessary to evoke the biological action.


Metabolism of the gastrointestinal peptide hormones. Results of investigations on the metabolism of gastrin, pentagastrin, cholecystokinin and secretin are discussed on the basis of data in the literature. Own results concerning the metabolism of 14C-pentagastrin are also described, out of which the significance of a biologically inactive metabolite is emphasized. This metabolite can be demonstrated in the circulation, and is excreted in the urine. As judged from the present state of investigations, this metabolite has a crucial importance in the metabolism of pentagastrin.


Clinical significance and perspectives of gastrointestinal peptide hormones. Present knowledge about gastrointestinal peptide hormones is discussed from three points of view: (a) diagnostic significance of these hormones; (b) states characterized by over-production or deficiency of peptide hormones; (c) clinical application and perspectives of gastrointestinal hormones. The data in the literature are subjected to a critical analysis; in  addition, the author's own experiments are discussed.


Correlational analysis of the effects of cold, hypoxia, and hypercapnia on heat production and body temperature in the newborn and the adolescent guinea pig, with complementary data on young adult guinea pigs, new-born rabbits and rats.


Oxygen uptake capacity of gills and skin in relation to body weight of the air-breathing siluroid fish, Clarias batrachus (Linn.). Oxygen consumption through gills and skin in relation to body weight was estimated in the air-breathing catfish, Clarias batrachus, under two experimental conditions, viz., (i) when access to air was allowed and (ii) when air-breathing was prevented. There was a positive correlation between VO2 (ml/hr) and body weight in both experimental conditions. Oxygen consumption (ml/hr) increased by a power of 0.869 when access to air was allowed whereas the power was slightly less (b = 0.841) when air-breathing was prevented. As the values for exponent (b) were less than 1.0, the weight specific VO2 (ml/kg/hr) decreased with increasing body weight. The decrease was more marked (b = - 0.180) in fishes which were not allowed air than in those where access to air was allowed (b = - 0.148). Under normal conditions of water and air-breathing the rate of VO2 (ml/kg/hr) via gills and skin from water ranged from 39.7 +/- 3.21 to 76.7 +/- 9.01 and this increased to 42.17 +/- 6.2 to 105.9 +/- 8.33 when air-breathing was prevented. The increase in the rate of VO2 was perhaps associated with the increase in the volume of water irrigating the gills per unit time.


Intestinal transport of fructose and glycine in Ophiocephalus punctatus. The regional variations in the absorption of fructose and glycine have been studied in the intestine and pyloric caeca of the fish, Ophiocephalus punctatus in vivo, by preparing intestinal sacs. Diffusion of amino acids out of the intracellular compartment of the intestine was observed in all the portions of the intestine. The pyloric caeca showed maximum transport of fructose. Absorption of fructose was slightly more in the posterior intestine than in the anterior intestinal sac. Maximum transport of glycine took place in the anterior intestinal sac. Pyloric caeca adsorbed more glycine than did the posterior sac. For both nutrients, the rate of uptake did not differ significantly in the upper and the lower portion of the intestine. In all the portions, the transport of fructose was more rapid than that of glycine. The experiments revealed that though the nutrients are absorbed in the entire length of the intestine, the quantity absorbed varies from one portion to another and is dependent on the nature of the compound absorbed.


Influence of amino acids on gastric emptying in young pigs. The effects of amino acids on gastric emptying and secretion were studied in four young conscious pigs provided with a chronic gastric cannula. A basal test meal of 500 ml containing 10 g citrus pectin, 17.5 g sucrose, 100 mg phenol red alone or with glycine, L- or DL-tryptophan, DL-methionine or L-glutamic acid was poured into the stomach and recovered 20 min later. Glycine at concentrations of 26.7--106.8 mM/l did significantly affect gastric emptying, although at the highest concentration gastric emptying appeared to slow down. L-lysine (41.0 mM/l), DL-methionine (40.3 mM/l or L-tryptophan (29.4 mM/l did not significantly affect the rate of stomach emptying; L-tryptophan increased the amount of Cl- and H+ secreted as compared with the basal diet alone. The effects of addition of the essential amino acids, L-lysine and DL-methionine, were compared with those of the nonessential glycine and L-glutamic acid. There was no significant difference in the rate of gastric emptying or secretion between them. The L and DL isomers of lysine and trypotphan were not found to differ significantly in their effects on gastric emptying and secretion.


Cortical synchronization induced by thermal stimulation of the preoptic area in immobilized rats. Cortical synchronization characterized by frontal spindles and occipital slow wave activity was induced by bilateral thermal stimulation of 2--4 degrees C of the preoptic area in unanaesthetized immobilized rats. The synchronization was demonstrated by ECoG and its power density spectra. The ECoG of the synchronization was similar to that of slow wave sleep. Switching off the thermal stimulation the original activity was recovered in most of the cases. Frontal spindle activity and slow wave activity recorded over the posterior cortex seemed to be dissociated to some degree. Low frequency stimulation at the points of thermal stimulation resulted in frequency following synchronization. In some cases electrical and thermal stimulation induced gradually developing seizure activity.


Effect of TSH and melatonin on thyroid activity in the rat. Melatonin and TSH, injected separately, caused no change of the blood thyroxine level at 30 min after treatment. Simultaneous or subsequent administration of the two hormones induced an increase of the level. Thus, melatonin is capable of potentiating acute, thyroxine mobilizing effect of TSH.


The frequency dependence of evoked and postsynaptic potentials in the somatosensory cortex of the cat. Intracellular records were made from neurones of the somatosensory cortex of cats, during stimulation of the ventrobasal complex of the thalamus. The aim of the experiments was to detect correlations between frequency dependence of surface evoked potentials and that of unit discharges. The amplitude of the surface evoked potential showed a strong diminution when the frequency of the thalamic stimulation was raised from 1 cps to 15 cps. In spite of this, frequency dependence in amplitude of unit discharges was never seen. As regards their frequency of occurrence the unit responses (full spikes, dendritic, postsynaptic potentials) behaved differently: a part of them showed increasing, another part gave decreasing occurrence, and the remaining portion did not change it. The authors conclude that temporal dispersion fails to give account for the frequency dependence, therefore further possibilities have to be examined.


Relation of unit spike discharges and evoked potentials in the auditory cortex of cats. Extracellular microelectrode recordings were made from the auditory cortex of anaesthetized cats during acoustic click stimulation. The microelectrode of low resistance allowed to record evoked field potentials and unit discharges simultaneously. In distant extracellular leads the relation of unit discharges and field potentials was equivocal. Near extracellular leads revealed that the antidromic invasion of the somadendritic membrane by excitation is a frequency dependent process (just as evoked field potentials) while spike potentials can reliably be elicited from the initial segment at high frequencies. It is assumed that the excitation spreading from the initial segment to the soma-dendritic membrane represents an important component of the evoked potentials, and their frequency dependence may be traced back to inhibitions activated by afferent impulses.


A device for evaluation of the dominant component of the EEG activity. A setup composed of a bioanalyser and additional electronic circuits is described allowing to measure the instantaneous frequency and amplitude of the dominant component of EEG activity. Besides recording the above values on paper by means of the EEG apparatus, functions of probability density of both the amplitude and the frequency of EEG activity are estimated on-line during preadjusted time intervals, and displayed on the screen of the bioanalyser. The mutual relationship between instantaneous EEG amplitude and frequency can be expressed in two-dimensional histograms displayed on a memory scope. The system described is suitable for laboratories having no possibility to use general purpose computer on-line.


Assumed identity of granulocyte-pyrogen (GP) and leukocytosis inducing factor (LIF). Pyrogen obtained from granulocytes induces granulocytosis in normal, endotoxin-tolerant and spinal animals alike, whereas heat treatment destroys the granulocytosis inducing effect. It is concluded that GP and LIF are identical, or nearly identical, factors.


Cardiovascular reactivity and neurogenic tone in unilateral renovascular hypertension in the rat. The role of some mechanisms in the development of hypertension due to unilateral renal artery stenosis is influenced by the presence or absence of the intact opposite kidney. In this paper: a) the cardiovascular reactivity (CR) to norepinephrine (NE) and b) the effect of a ganglionic blocker (pentolinium, P) during the early (first two weeks) and later periods (ten weeks) of hypertension elicited by unilateral renal ischemia in the presence of the untouched contralateral kidney in the rat have been reported. Neither the threshold doses nor the dose-pressor response curves have shown a greater reactivity of the cardiovascular system to NE in this specific type of renovascular hypertension. An increase in the activity of the nervous system apparently contributes in early and late periods to the fuller development of high arterial pressure (AP).


Electronic system for the obtention of phase plane curves for action potentials. This system is designed for the amplification of the bioelectrical signal, obtention of its derivative in respect to time, automatic brightness control for both beams of the oscilloscope, and stability. The system includes a central measuring section which is a modified Tektronix oscilloscope type 565. Automatic control of brightness as a function of sweeping speed allows photographic recordings.


Influence of sympathectomy on survival after coronary occlusion.


Automatic microelectrode compensator (AMC). A solid-state system designed for compensation of asymmetry potentials between microelectrodes, for bioelectric measurements, is described. Advantages over the manual compensator are the following: synchronic compensation of electrodes, application to automatized systems against aging effects in electordes, high speed, and elimination of general muisances of manual circuits. Besides, the system allows to measure digitally the polarization difference and resting membrane potential with precision of +/- 1 mV.


Automatic silver-plater of electrodes. Reference silver-silver chloride electrodes. The silver-plater developed for the preparation of silver-silver chloride electrodes attains constancy in electrochemical characteristics, circulating current during exactly identical periods with inverted polarity.


Effects of prostaglandins upon hair growth in mice. The effect of prostaglandins upon the diffuse hair wave induced by gonadectomy was studied in male C3H mice. The drugs used were Prostaglandin E1 and Prostaglandin F2alpha, tromethamine salt, which were administered twice a day intraperitoneally during 22 days, in daily doses from 1 to 6 micrograms. The animals had their back clipped and were castrated at the beginning of each experiment. At the end of the 22 day experimental period while all the castrated control mice were completely covered by hair, in the castrated prostaglandin treated mice a marked inhibition of the hair growth was noticed.


Effect of diabetes on growth of mouse lymphoma P1798.


The effect of adrenaline, tyramine and guanethidine on one-way step-up avoidance.


Metabolic responses to catecholamines.

Metabolic responses to catecholamines. Isoproterenol and propranolol, in a single dose, caused hyperglycemia after 15 and 30 min, either in the conscious rat or in the anesthetized dog. In this latter species no modifications of the serum potassium were observed. Adrenaline, 5 microgram/kg, iv provoked hyperglycemia at the same intervals and hyperkalemia at min 1st and 2nd with further hypokalemia until 90 min. The beta-adrenergic blocker, sotalol, 5 mg/kg, iv, administered prior to adrenaline suppressed the increase in glycemia and the late decrease in serum potassium, but not the early hyperkalemia. In the isolated hind limb of the dog the intra femoral artery administration of adrenaline, 3 microgram/kg, produced similar hyperglycemia either in the artery or in the femoral vein, starting from the 15 min. Contrarily, the serum concentration of potassium was significantly less in the vein than in the artery at the 1st min. These findings suggest that different receptors are involved in the glucose and potassium response to adrenaline, and the skeletal muscle plays an important role in the regulation of the early hyperkalemia.


Caudate-putamen and globus pallidus influences on a visceral reflex. The electrical stimulation of the rostral striatum in rats has two different influences on bladder motility. Stimulus applied to the dorsomedial caudoputamen nucleus elicited vesical contractions and increased excitability of micturition reflex. Stimulation of the ventromedial caudoputamen suppressed detrusor contractions and increased the micturition threshold. Similar inhibitory effects were found by electrical stimulation of the globus pallidus. The possible mechanisms involved and the relationship with the clinical finding of bladder dysfunction in patients with extrapyramidal disease, are discussed in view of their anatomical and functional relationships to the vesicomotor centers.


The effect of tranylcypromine on the body temperature of rabbits untreated and pretreated with para-chlorphenylalanine.


Immunoreactive insulin in ischaemic heart disease.


Effect of hypocalcic media on chemical sensitivity of toad sartorius muscle. Membrane potential and spike activity were recorded through intracellular electrodes from toad sartorius muscles superfused in Krebs solution. When this was replaced by a hypocalcic medium (1/20 of normal Krebs Ca2+ concentration), muscles showed extrajunctional sensitivity to acetylcholine, histamine and noradrenaline applied iontophoretically. Normal sensitivity was restored when muscles were bathed in normal calcium media. Changes in chemical excitability of the fibers immersed in hypocalcic solution were related to decreased input membrane resistance. These observations emphasize the role played by Ca2+ on electrical stability and chemical sensitivity of skeletal muscle membrane.


Secondary structure of glycosaminoglycans. It has been shown by X-ray diffraction and chiroptic spectroscopy that the glycosaminoglycans in connective tissue develop a helical structure; this induces the coordination of polycations and scleroproteins in their environment polycations and promotes or inhibites fibrillary aggregation. Analysis of the ORD and CD spectra of various glycosaminoglycans, as well as those of oversulphated and desulphated preparations allowed the following conclusions concerning the secondary structure. 1. For the helical structure of glycosaminoglycans a chain-length of 120 to 150 A and the presence of at least one sulphate group per two disaccharides are required. The helical structure is not influenced by binding to the protein skeleton, e.g. in proteoglycans. 2. Chiroptical properties of the glycosaminoglycan--either in themselves, or if their methylene blue complexes are studied--are determined by the degree of their sulphatation. 3. The degree of sulphatation determines the secondary structure of the molecule and thus also its biological properties. Fibrillogenesis in vitro and probably also in vivo are determined by the strength of the bond between collagen and glycosaminoglycans, in addition to the effect inducing the coordination. Structural analysis of the polysaccharides supports the coordinated fibrillary structure of GAG, as already assumed on the basis of polarization microscopic methods.


Nodular thickening of peritubular basement membranes in diabetic kidneys. A conspicious renal change associated with diabetes is the thickening of the basement membranes. This was generally considered diffuse and little attention was paid to its nodular character. In the present study of the nodules, their topo-optical reation, electron microscopic fine structure as well as their trypsin digestibility after periodic acid-sulphatation revealed their basement membrane character. The increased basophilia obtained by the aldehyde-bisulphite-toluidine blue reaction indicated an increased amount of sugar components, whereas the decreased double refraction and the red polarization colours a decrease of the micellary arrangement. The change is mostly observed in diabetics; the specific localization around the Henle loops may refer eventual hyperosmolar effects, whereas the nodular character points to the role of local factors.


Neonatal hepatitis: histological and differential diagnostic aspects. The morphological pecularities of neonatal hepatitis are discussed on the basis of biopsy in 41 cases. The manifestations are similar to those of virus hepatitis in more advanced age, with certain quantitative and qualitative differences. The obligatory presence of multinucleated hepatic giant cells is denied, but a peculiar cell type with large bizarre nucleus was found pathognomic. The questions of differential diagnosis are discussed and the importance of liver biopsy is emphasized.


Cell surface properties of L1210 leukaemia cells. The surface properties of vincristine-colchicine sensitive and resistant L1210 leukaemic cells have been studied using concanavalin A mediated agglutination assay as well as electron microscopic visualization of concanavalin A receptors. 3H-colchicine uptake by the sensitive and resistant lines has also been compared. The resistant L1210 leukaemic cells proved less agglutinable than the sensitive ones at the same concanavalin A concentration. Previous treatments with either colchicine, vincristine or chlorpromazine caused a marked decrease in the agglutinability of the sensitive L1210 leukaemic cells, while agglutination of the resistant ones was lowered slightly by the same treatments. The 3H-colchicine uptake of the sensitive cells was three times higher than that of the resistant ones.


Infantile and juvenile cirrhosis of the liver. In 14 years necropsy material 12 cases of infantile and 2 of juvenile liver cirrhosis occurred. In early infancy, diffuse cirrhosis is the most characteristic, whereas in more advanced age cirrhosis of irregular type is more frequent. The posthepatitic, secondary biliary, metabolic and cryptogenic cirrhoses are discussed from aetiological aspects.


Bioassay of blood-born tumour cells on in vivo and in vitro systems. Viability and biological integrity of tumour cells circulating in the blood were studied in an experimental system using bioassay methods. Results of subcutaneous and intravenous retransplantation as well as explantation of the blood obtained from tumour-bearing animals previously receiving intravenous inoculation of tumour cell suspension (Yoshida sarcoma, Walker 256 carcinosarcoma and DMBA-induced myeloid ascitic leukaemia), revealed that the tumour cells detected in the blood are not only viable at the time of their transportation in the blood-stream but are also in possession of biologic potentials to proliferate and establish metastatic growths in organs.


The role of heterogeneity of tumour cell populations in biological activity and sensitivity to chemotherapeutics. The biological activity of a rat ascites leukaemia subline isolated by isopycnic centrifugation was compared to that of the original tumour both in vivo and in vitro. The subline had different marker chromosomes, a longer cell cycle time and decreased sensitivity to vinblastine as compared to the original tumour.


Examination of curettage material for the diagnosis of ectopic pregnancy. The histological characteristics of curettage material are described which raise the suspicion of ectopic pregnancy. In the period 1966 to 1975, 464 surgically removed pregnant uterine tubes and in 143 patients curettage material was also examined. On the basis of the curettage material, the suspicion of ectopic pregnancy arose in 109 cases (76%). In 34 curettage materials (24%) no histological sign referring to ectopic pregnancy was detected. Thus in three-fourth of cases examination of the endometrial specimen was of diagnostic value and indicated the necessity of surgery.


Basal cell carcinomas of the eyelid and the periorbital region.


A study of hyperlipidaemia induced by ascites tumours. In mice four stages of hyperlipidaemia induced by Ehrlich ascites tumour could be distinguished. Hyperlipidaemia is characterized mainly by increased serum VLDL content accompanied by high triglyceride concentration. The only exception was the regressive stage III where the serum lipid level (VLDL) has temporarily decreased. From the results obtained with the simultaneous examination of the liver, mesenteric fat tissue, tumour cell and ascites plasma lipids, it may be concluded that the endogeneous fat mobilization induced by tumour cells via increased VLDL synthesis and secretion of liver will lead to hyperlipidaemia and to the total depletion of the lipid stores. Rapid and reversible fall in lipid level following the withdrawal of ascites fluid in tumorous animals demonstrated clearly the direct effect of the tumour cells on lipid-lipoprotein metabolism of the host organism.


Pathology of human precanceroses. Skin, larynx and breast.


Avian aldolases. II. Primary structure of fructose-1,6-diphosphate aldolase from Gallus gallus domesticus and Columba liver breast muscle.


Clinical observations on erythrocyte sedimentation rate mixed with tuberculoproteins.


A study on the cases with negative disc exploration.


Gastroscopic and histologic studies on recurrence and intractability of gastric ulcer.


Snail control and some significant control projects, review.


Studies on the possiblilty of production of immunity against schistosomiasis in experimental animals. In the present work antigenization with somatic and metabolic antigens of adult Schistosoma mansoni worms, their cercariae or eggs has been tried. The study of the immunoglobulin patterns in infected and vaccinated non infected mice showed increase in IgG in all animals, increase in IgM only in the infected and IgA was frequently increased in mice that received metabolic or egg antigens. As regards the degree of resistance of vaccinated animals to S. mansoni infection, 2 groups gave prominent results. 1st the group vaccinated with Schistosoma mansonmetabolic worm antigen in which we got: -- Reduction in the worm load. -- Delayed ovulation and reduction in the number of ova/24 hr stools and/g of liver tissue. -- The histopathological picture of the liver was the best. 2nd the group vaccinated with egg antigen: Although it showed delayed ovulation and reduction in the ova count per 4 hr stools and/g liver tissue, yet the histopathological picture was the worst showing the largest granulomata.


Leucine aminopeptidase, Significance of serum elevation in bilharziasis. Leucine aminopeptidase (LAP) activity and creatinine concentration was estimated in serum of twenty five normal adult subjects, twenty four cases with active urinary bilharziasis, eleven cases with active intestinal bilharziasis, ten cases with mixed bilharzial infection (urinary and intestinal), fourteen bilharzial cases with clinical hepato-splenomegaly of bilharzial etiology, thirteen bilharzial cases with clinical hepatosplenomegaly and ascites, and twelve cases with cancer bladder of bilharzial etiology. Significant elevation in the serum enzyme level was found in all bilharzial cases which generally ran parallel to the course of the disease, being more marked in hepatosplenomegalic cases than in cases which showed no clinical signs of liver or spleen involvement. However, it was observed that the enzyme level was lower ascitic than in nonascitic cases. For serum creatinine concentration, no significant variation from normal value was observed in the group of patients with active urinary, active intestinal, mixed infections of bilharziasis and bilharzial hepatosplenomegalic cases. However, a very highly significant decrease in serum creatinine concentration was observed in the group of patients with bilharzial hepatosplenomegaly and ascites. Serum LAP activity and creatinine concentration in the group of patients with cancer bladder of bilharzial etiology showed no statistical variation from normal values.


Urinary leucine aminopeptidase in bilharziasis. Leucine aminopeptidase activity and total protein concentration was estimated in the fresh early morning sample of urine of twenty five normal adult subjects, twenty four cases with active urinary bilharziasis, eleven cases with active intestinal bilharziasis, ten cases with mixed bilharzial infection (urinary and intestinal), fourteen bilharzial cases with clinical hepatosplenomegaly of bilharzial etiology, thirteen bilharzial cases with clinical hepatosplenomegaly and ascites, and twelve cases with cancer bladder of bilharzial etiology. It was found that there is a significant increase in LAP activity in the urine of all groups of patients studied. Again this increase ran parallel to the course of the disease in bilharzial cases. Urine protein concentration was found to be increased significantly in all groups of bilharzial cases studied with the highest value in urine of patients with cancer bladder of bilharzial etiology. However no direct correlation was found between urinary LAP level and protein concentration either within the individual cases or within the different groups.


Chemical control of the sea lamprey: the addition of a chemical to the environment. Construction of the Welland Canal enabled shipping to by-pass Niagara Falls and enter the upper Great Lakes and also eliminated the barrier to the entry to the lakes by the sea lamprey (Petromyzon marinus Linnaeus). Within forty years the commercial fisheries of the Great Lakes was almost eliminated by this parasitic cyclostome. A search for selective chemical control of the sea lamprey was undertaken in the 1950's and culminated with the discovery of TFM (3-Trifluoromethyl-4-nitrophenol). At the request of the International Great Lakes Fishery Commission, the Bureau of Sport Fisheries and Wildlife undertook to assess the hazard of TFM to the aquatic ecosystem, to humans as well as to fish and wildlife. Studies were undertaken in Bureau laboratories as well as by contracts with university and private laboratories. Results of these studies to-date indicate that this material is not subject to biomagnification and does not pose a hazard to man or the the environment.


Recent chemical and biological studies on natural products at the Central Drug Research Institute, Lucknow.


Long-lived radionuclides in food: 90Sr and 137Cs in the Israel diet. Routine monitoring of Strontium-90 and Cesium-137 levels in the main classes of food consumed in Israel has been carried out for a number of years. Calculations have been made of the intake of these isotopes by various groups in the Israel population using Food Consumption Survey data made available by the Israel Central Bureau of Statistics. More detailed analyses have been carried out to calculate the exposure of groups selected on the basis of demographic, economic and family-size criteria. This was carried out in an attempt to detect "critical groups" in the population who might be overly exposed. Results of these data indicate that in the case of Israel the monitoring of a single food item such as milk or the extrapolation of local fallout data cannot be relied on to give an estimate of radionuclide exposure through food. The relation between high consumption groups and the intake of "average" consumers is discussed in detail and the use of the statistical models developed in these studies for the estimates of radionuclide exposure during periods of high fallout levels which occurred in the past and possible applications with regard to other environmental contaminants is discussed.


Selenium content of some foodstuffs and other environmental samples in a mineralized area of Italy. No data are available on Selenium concentration in the Italian environment in spite of its importance as an essential and toxic element. In order to get some information about the concentration and distribution of this element in the environment and in the food-chain, a survey has been made in the Monte Amiata area (Toscana, Italy). Such an area was selected for this study owing to its large mineralization due to many elements (e.g. Fe, Cu, Ag, Sb and particularly Hg). The selenium content has been determined by thermal neutron activaltion analysis and a large volume high-resolution Ge-Li gamma ray spectrometer connected on line to a DEC PDP 8/L computer. The instrumental method requires neither a chemical separation technique nor a pre-or post-concentration of Selenium. The Selenium concentration for wet weight in foodstuffs ranges between a value of 0.008 ppm in milk and a value of 0.2 ppm in chicken. The average Se intake for the population living in the area under investigation has been also estimated. A critical discussion of the data is reported together with a comparison with the Se concentration values referring to other countries.


ECDIN, an EC data bank for environmental chemicals. All environmentally relevant information is computer stored for each organic chemical manufactured in quantities above 1.000 tons per year (whether it is considered as potentially toxic or not), known toxic chemicals (independently of their production figures) and certain classes of inorganic chemicals. The main data fields ("attributes" for each chemical) are: 1. substance identification and physicochemical data; 2. production and use data; 3. handling, transportation, and disposal; 4. environmental involvement (breakdown under biotic and abiotic conditions, average concentrations in environmental matrices); 5. biological effects and toxicity; 6. protection standards, administrative and regulatory data. Structure and substructure codification permits screening for substances with similar structures and coupling to GC-MS-computer analysis units with full or approximative structure outputs. A pilot project has been started permitting queries via direct and inverted file. It will include all available information on a limited number of compounds (some 5.000) from different chemical and use classes. The further software development foresees automatic indexing, automatic encoding of formatted data, automatic query formulation and search on a probabilistic basis for natural-language source data. Easy data flow is ensured by a networking between the central unit at the EC research Centre in Ispra (Italy) and specialised national data centres (ECDIN = European Chemicals Data and Information Network).


Bioaccumulation of 14C-DDT in a small pond.


Multielement characterization of atmospheric aerosols by instrumental neutron activation analysis and X-ray fluorescence analysis.


Radioisotope techniques in delineation of the environmental behavior of cadmium. Radioisotope techniques are being developed and utilized at Oak Ridge National Laboratory (ORNL) for evaluating the environmental behavior of toxic elements such as cadmium in aquatic and terrestrial ecosystems. Tracer techniques using 109Cd in microcosm, field plot, and stream systems are providing information on biogeochemical cycling and distribution of cadmium in the environment. Parameters being measured include adsorption capacity for cadmium in mineral soils and sediments; uptake rates of cadmium in various plant species from both soils and nutrient solutions as affected by pH, competing cations, and chemical form of cadmium; and distribution of cadmium in various components of both aquatic and terrestrial ecosystems following application of 109Cd to soil vegetation, or directly to streams. Food chain parameters being estimated with 109Cd include uptake, assimilation, and turnover by both aquatic and terrestrial organisms. Information obtained in these radiotracer studies is providing insight into the behavior of cadmium in aquatic and terrestrial ecosystems, especially transport rates of cadmium and potential biomagnification or dilution in food chains. The factors which influence the incorporation of cadmium into vegetative material as well as those affecting residence time in ecosystems have been identified. Use of 109Cd also has permitted evaluation of a cadmium specific electrode as a tool for rapid assay of free cadmium ions in soil solutions.


Global inputs and trends of chemical residues in the biosphere.


Technology assessment on the use of pesticides. The extensive use of pesticides in Japanese agriculture was studied as a problem-oriented case of technology assessment with an aim to contribute to the establishment of appropriate procedures of assessing real and potential impacts which modern technologies have or may have on health, industries, economy, society and on the environment and to obtain clues to the development of safer use of pesticides. Direct and indirect impacts, both real and potential, favourable and adverse, were intended to identify and evaluate systematically and comprehensively as their cause and effect sequences were studied. Adverse impacts were divided into tradable and untradable categories, untradable adverse impacts were related mostly to human health. As to the evaluation of impacts, it was suggested that the size of area and population affected, irreversibility and controllability of the impact be considered. It was recognized as urgent and requisite to develop and establish a more efficient and satisfactory method of testing the safety of pesticides and their metabolites over a wider spectrum of organisms and with respect to newer aspects of toxicology as mutagenesis, teratogenesis and cancerogenesis.


Biological control and environmental quality.


Natural enemies as a control agent of pests and the environmental complexity from the theoretical and experimental points of view.


New approaches: pheromones and related chemicals in some USDA insect control programs.


Use and field application of pheromones in orchard pest management programs. Pheromones are recognized as potentially valuable tools in orchard pest management. Their most immediate practical application is in pest monitoring to indicate the need for chemical control. In this role, they can have considerable impact in reduction of pesticide output.


Approaches to insect control based on chemical ecology--case studies. In this article two attempted approaches to control a stored product insect, azuki bean weevil, are discussed based on host selection study and oviposition ecology. The latter study indicated the presence of an oviposition marker, a new kind of pheromone, which was derived from the weevil and was lipid in nature, and suggested its possible use as an oviposition inhibitor.


Embryological aspects of environmental toxicology: relation between environmental protection and the successful conservation of species in the view of experimental embryology.


Modern science for future environment and social university youth activities.


Experiments on the effect of carbon monoxide on aminolevulinic acid dehydrase (ALAD). The inverse relationship between blood lead concentration and aminolevulinic acid dehydrase (ALAD) is well known. Recently, it has been suggested that a similar relationship exists between carboxyhemoglobin (COHb) and ALAD activity. This study was undertaken to examine more closely the possible effect of carbon monoxide on ALAD. Blood from 19 human volunteers was analyzed for both carboxyhemoglobin and ALAD activity. Smokers had significantly lower concentrations of ALAD than nonsmokers and a rise in carboxyhemoglobin concentration was assocaited with a fall in ALAD activity. The in vitro bubbling of carbon monoxide into human blood did not significantly effect ALAD activity. Four groups of rats (10 per group) wre exposed to carbon monoxide or dietary lead acetate according to the following design: (I) Control--no Pb or CO; (II) 500 ppm Pb acetate in diet; (III) 250 ppm CO four hours/day X 5 days/week X 4 weeks; (IV) Both Pb and CO. Analysis of the rat data showed a significant depression of ALAD by lead. The activity of ALAD in the rats exposed to CO was significantly increased suggesting the possibility of an adaptive phenomenon.


Chyluria pathologenesis and diagnosis.

Chyluria pathologenesis and diagnosis.


Effect of glucose on the action of some anaesthetics.


Hycanthone in experimental schistosomiasis studies on liver pathology in mice treated with one and two doses.


Papillary carcinoma of the thyroid, pathological aspects in relation to surgical management.


Evaluation of some parameters of lead exposure and possible correlation between them.


Food additives.

Food additives.


The effect of "thalamonal" on the electromyographic activity of the stomach.


Serum and urinary immunochemical studies in primary nephrotic syndrome.


Serum transaminases in late pregnancy and after labour.


Treatment of lichen planus with bactrim.


Personal concepts and trends in management of cancer breast.


Subacute extradural haematoma.

Subacute extradural haematoma.


Sexual problems associated with infertility.


Hut entry and exit by Anopheles gambiae and Anopheles funestus in an unsprayed village near Kaduna in Northern Nigeria. Hut entry and exit by An. gambiae and An. funestus were studied in an unsprayed village near Kaduna in Northern Nigeria. A high turn-over of indoor resting mosquitoes of all blood digestion stages was noted throughout the night. The behaviour of both species was similar. The entry pattern was more uniform than the exit which showed two distinct peaks around sunset and after midnight. Most of the daytime resting mosquitoes left the huts during the first hour after sunset, and therefore the mosquitoes escaping during the subsequent hours of the night were mainly those that had entered the same night and spent only a limited period inside the huts. The significance of the movement of mosquitoes is discussed in relation to entomological evaluation of the impact of residual insecticides.


Linkage study on dieldrin resistance and an inversion on the second chromosome of Anopheles stephensi. In a study on the linkage between the gene for dieldrin resistance and an inversion on the second chromosome in Anopheles stephensi, the two factors were found to assort independently. As dieldrin resistance can be assigned either to the third chromosome, or to a position on the second chromosome more than 50 cross-over units from the inversion.


Prevention of cardiovascular disease: an urgent Canadian problem.


Transmission patterns of onchocerciasis in southwest Ethiopia. The three transmission patterns found till now in Ethiopia are described. The forest area contains ideal conditions for the breeding of Simulium, but the sparse human population makes the transmission rate low. Only a few people were found to be infected. The subsistence crop farming area is a less ideal habitat for the vector due to the scarcity of bush and shade. However the presence of a largest population can explain the slightly higher prevalence of the disease than in the forest. The coffee farming area is densely populated, rich in bush and shade, with heavy rainfall and numerous rivers. Here the number of Simulium and their chance to reach the infective stages, are considered to be high. A high prevalence of the disease was found in this zone.


The future is now.

The future is now.


Depression after myocardial infarction.

Depression after myocardial infarction.


Propranolol therapy in the perioperative period.


Fomites in vaginitis.

Fomites in vaginitis.


Sudden death in asthma.

Sudden death in asthma.


The salivary gland chromosomes of Anopheles labranchiae Falleroni 1926. The salivary gland chromosomes of Anopheles labranchiae Falleroni, 1926 are described and figured. A. labranchiae is the sibling species of the maculipennis complex most closely related to A. atroparvus and the map of A. labranchiae is generally comparable to that of A. atroparvus. The chromosomal areas which are asynaptic in the hybrids between the two species are also described.


Caffeine content of beverages as consumed. Quantitative analysis of beverages prepared at home by staff of the Addiction Research Foundation revealed a lower and much more variable caffeine content of both tea and coffee than had been reported in earlier studies, most of which were based on analysis of laboratory-prepared beverages. Median caffeine concentration of 37 home-prepared samples of tea was 27 mg per cup (range, 8 to 91 mg); for 46 coffee samples the median concentration was 74 mg per cup (range, 29 to 176 mg). If tea and coffee as drunk contain less caffeine than generally supposed, the potency of caffeine may be greater than commonly realized, as may the relative caffeine content of certain commercial preparations, including chocolate and colas. The substantial variation in caffeine content emphasizes the need to establish actual caffeine intake in clinical, epidemiologic and experimental investigations of caffeine effects.


New view offered of cause of postoperative sepsis.


Technique evaluation of foster care in chronic psychiatric disorders. Foster care received by 178 patients with chronic psychiatric disorders discharged from Hamilton Psychiatric Hospital in the years 1966 through 1969 was studied by technique evaluation. Residents were followed for 3 years by means of health records. The achievement of operational objectives of the program (Homes for Special Care) was compared with two types of outcome--emergency readmission to hospital and discharge to the community. Emergency readmission was associated with rural location of the foster home, inferior quality of the home operator and smaller size (i.e., fewer residents) of the home. Discharge to the community was more common among younger, female residents whose previous psychiatric hospitalization had been relatively brief. In general, prescription audit was not a fruitful way of evaluating quality of health care.


In vitro estrogen-binding by human breast carcinomas. Patients whose breast carcinomas possess only low concentrations of a receptor molecule that binds estrogens with high affinity are unlikely to respond to hormonal manipulative therapy when the disease recurs. The estrogen-binding capacity of 106 breast carcinomas was measured by an in vitro method and was expressed per milligram wet weight and in some cases related to the concentration of deoxyribonucleic acid (DNA) of the tumours. The ability of tumors to bind 3H-estradiol ranged from 0 to 1.3 fm/mg in pre- and perenopausal women, and from 0 to 16.8 fm/mg in postmenopausal women. Menopausal status or serum concentrations of endogenous estrogen, or both, should therefore be considered when tumours are classified into low and high estrogen-binding capacity. It is not necessary to carry out Scatchard analysis for every tumour, and expressing estradiol binding on the basis of DNA concentration may be preferable to expressing in on a wet-weight basis.


Decreased anion gap associated with monoclonal and pseudomonoclonal gammopathy. Nine patients with monoclonal and one with pseudomonoclonal gammopathy were found to have a decreased anion gap. Eight of the patients had multiple myeloma, one has plasma cell leukemia and one had chronic active hepatitis. In all of the the decreased anion gap was associated with an increased concentration of IgG greater than 5 g/dl.


Carcinogens are not that common.

Carcinogens are not that common.


Symposium reviews how geriatric patients are different.


Ultrasound providing valuable information in pregnancy.


Finding and helping the deprived child.

Finding and helping the deprived child.


PEI's best buy hospital registers no sale.


The value of esophagoscopy in patients with bilharzial hepatic fibrosis.


A simple tube test for assessing antibiotic sensitivity of certain anaerobic bacteria.


Pharmacological evaluation of xanthinol nicotinate (complamin).


A new sensitive Schistoma antigen for the diagnosis of schistosomiasis by the intradermal test.


Inhibitory effect of metformin on L-B-phenyl alanine-125 absorption in man.


Effect of nicotinic acid on blood sugar, its character and possible mechanism of action.


Assessment of serum copper level and ceruloplasmin activity in toxaemia of pregnancy.


Plasma immunoreactive insulin (IRI) and glucose tolerance in bilharzial hepatic fibrosis.


Anti-leucocyte and anti-platelet iso- and autoantibodies in leukemias and malignant lymphomas.


The relation of seminal acid phosphatase to fertility.


Diagnostic value of Hagens test in inferitility.


The absent symphysis syndrome.

The absent symphysis syndrome.


A method for electrophysiologic study of drug actions on the ciliary muscle "in vivo".


Evaluation of salivary electrolytes in digitalis toxicity.


Hemostasis in the Egyptian female in abortion with a case report. Hemostatic factors in cases of early pregnancy bleeding were estimated in 16 patients pregnant for 8-12 weeks and compared with established mean values for normal pregnancies (controls).  During abortion there was a significant decrease in platelet count and clot retraction and an increase in capillary fragility, indicating utilization of hemostatic factors.  However, the whole blood clotting time was shortened.  Subsequent recovery in the postabortive period occurred at a slower rate than that of the recovery previously established for normal delivery (mean platelet count, 268+ or -52 times 10(3)/cu. cm 6-10 days after abortion;  410+ or -103 times 10(3) in corresponding puerperal period).  The state of enhanced hemostasis observed 6-10 days after normal labor did not manifest fully after abortion.  Abortion resulted in a significant reduction of hemoglobin percentage which was maintained for 10 days.  A depressed state of hemopoiesis in abortion might be in part responsible for the persistence of lowered hemoglobin reported in the postabortive period.  A case of hemopoietic failure as a result of pregnancy is described, and it is suggested that therapeutic abortion in such conditions may be life-saving.


Zinc in serum and urine of diabetics.

Zinc in serum and urine of diabetics.


An investigation on the decarboxylase activity of human and animal diphtheroids.


Some presentations of asymptomatic urinary tract infection.


Tinidazole therapy in lambliasis and amoebiasis.


Assessment of serum arginase activity in intestinal and hepatic schistosomiasis.


Cryptogenic malignant melanoma of the head and neck.


New concepts and trends in hand infection.


Thalamonal with local infiltration anaesthesia in plastic surgery.


Effect of intravenous citanest on adrenaline-induced ventricular fibrillation during chloroform anaesthesia.


Health Maintenance Organizations (HMOs)--legal hazards.


Hearing, traumatic deafness: medicolegal implications.


Spotlights on the Rod Sketch Theory.

Spotlights on the Rod Sketch Theory.


The advent of legal abortion and surgical abortion techniques. The 4 principle abortion techniques are 1) dilation and curettage (D and C), 2) vacuum aspiration, 3) amniocentesis (saline injection), and 4) hysterotomy.  The curettage and vacuum methods are used in the first trimester of pregnancy.  Both require dilation of the cervix.  The fetal material is removed with a curette or suction applied to the uterine wall.  First trimester abortions usually do not require hospitalization.  No abortion should be performed between 12-14 weeks.  After 14 weeks, ideally 20 weeks, amniocentesis is the preferred method.  It is a more complicated procedure involving removal of amniotic fluid and replacement with hypertonic saline solution, resulting in fetal death and expulsion.  Oxytocin may also be used to induce labor.  Reported complications run from 5-15% Hospitalization is up to 48 hours.  A hysterotomy requires exposure of the uterus by a surgical opening in the abdomen.  The uterus is opened and fetal material delivered.  Once a hysterotomy is performed, all other pregnancies must be delivered by caesarean section.  The psychological considerations of abortion have not been carefully studied under the new laws.  No clear consensus exists as to the psychological aftereffect of abortion.  If a woman desires an abortion and does not receive one, there is a likelihood of psychological effects, as for a woman who does not desire an abortion and receives one.  Conflict occurs only when a woman is not sure of her own decision.  Moral considerations imposed by abortion service personnel will also set up a conflict for the patient.  The abortion seeker must know her own attitudes regarding the sanctity of life and her social role as a woman and be psychologically prepared to actually arrange for the operation.


A medical "brief" in a malpractice case.


Blood groups in bastardy, paternity, heredity and criminal cases.


The high-low contract: a trial by chance.


Analysis of neocortical electrical responses evoked by stimulation of various hypothalamic structures. Evoked potentials (EPs) and extracellular unit activity were recorded in various parts of the neocortex of unanesthetized cats immobilized with succinylcholine. During hippocampal stimulation EPs of maximal amplitude are recorded in the anterior sigmoid gyrus with a latent period (LP) of 0.5-2.5 msec. The first component is a fast positive spike with a duration of about 1.5 msec. In the middle suprasylvian gyrus, hypothalamo-cortical (HC) EPs have a monophasic surface-negative configuration and are recorded after an LP of 2-6 msec. HC EPS in the anterior sigmoid gyrus can bind a rhythm of stimulation of up to 200 pulses/sec and are characterized not only by high functional mobility, but also by the property of posttetanic potentiation. The main positive wave appears to a testing stimulus with an interval of 7-10 msec. Complete recovery of all components of HC EP takes place at an interval of 50-150 msec. In response to the conditioning stimulus a biphasic change in excitability of the neurons is observed in the association areas of the anterior sigmoid and middle suprasylvian gyri of the neocortex. The maximal number of responding neurons in the deep layers of the cortex discharges in the period of development of the initial spike and the main positive wave of the HC EP.


Analysis of the role of central noradrenergic structures in memory trace recall. During the reproduction of a conditioned passive avoidance response by albino rats and also during the action of a conditioning stimulus in animals trained by invariable reinforcement of the conditioning stimulus, a significant decrease was found in the noradrenalin content in nuclei of the limbic system and anterior hypothalamus compared with the control. In untrained animals, and also in trained animals subjected to repeated testing in the course of 4 days, the decrease in the noradrenalin content was less marked. Reproduction of the conditioned active avoidence response in a shuttle box did not induce changes in the noradrenalin content.


Diagnostic importance and possible mechanism of a dynamic characteristic of the EEG in brain tumors.


Senile amyloidosis in schizophrenia.

Senile amyloidosis in schizophrenia.


The spleen function of trapping the aged erythrocytes.


Changes in erythrocyte specific gravity and osmotic fragility due to exercise.


Diagnostic application of thyroid lymphography for nodular thyroid lesions.


Some properties of rapidly labelled ribonucleic acid associated with light ribonucleoprotein particles from young and adult rat brains.


Senile changes in the physical strength and the skin, and their relation to vital prognosis.


A long-term observation of the environmental conditions of a factory manufacturing lead arsenate as an insecticide (studies on lead arsenate poisoning, Part 1).


Therapeutic spectrum of sulpiride, haloperidol and lithium: a graphic representation by clinical observation.


Induction of an allergic gastritis in guinea pigs sensitized to 2, 4-dinitrochlorobenzene--Experimental studies on gastritis from the viewpoint of contact allergy.


The autonomic basis of certain forms of activity in local lesions of the limbico-reticular complex (mathematical analysis of the cardiac rhythm).


Interaction between peripheral motoneurons of antagonist muscles in normal subjects and spastic hemiparesis studied by the H-reflex method.


Family formation by patients with schizophrenia and manic-depressive psychosis based on the results of an epidemiological study of birth rates.


Functional asymmetry of the cerebral hemispheres in clinical types of schizophrenia.


EEG patterns in children aged 6-7 years during formation of a complex motor-visual skill.


Comparison of the role of the first and second cortical somatosensory areas in the conditioned behavior of animals.


Predicting the frequency of episodes of schizophrenia (results of an epidemiological investigation).


Dynamics of evoked potentials in the dog auditory cortex during the formation of goal-directed behavior after ablation of the medial geniculate bodies.


Heterogeneity of the neuron membrane of Helix pomatia and its possible role in the organization of receptive fields.


Correlation between neocortical and hippocampal biopotentials in lobectomized dogs.


Functional maturation of the visula cortex during prenatal development of the cat.


Intellectual development and school achievement of youths 12-17 years: demographic and socioeconomic factors.


Histochemical study of lipid absorption in two teleost fishes. Absorption of lipid in the different regions of the intestine of two teleost fishes has been studied histochemically at different time intervals of 4, 10 and 20 h. All the three portions of the intestine have the absorptive capacity. However, it varies from one region to another. The lipid has been absorbed in the form of lipoid particles and lipoid goblets. It is also recorded here that some lipoid droplets are absorbed by pinocytosis across the brush border membrane. The lipid absorbed was localized in the brush border cells, in intercellular spaces of connective tissue, in submucosa, in between serosa and muscularis, in the lumen of blood capillaries and mesentric lymphatic vessels but was absent in muscularis and serosa.


Studies on the oogonial cycle in Puntius ticto (Ham.) II. Formation of mature follicles. During the formation of maturing oocyte and mature follicle most of the changes are observed in the ooplasm while nucleus gets reduced in size and finally vanishes in the ooplasm. Few of the nuclei migrate into the ooplasm (yolk nucleus) which is responsible for the yolk accumulation. The mature follicle is covered by the theca, granulosa and zona rediata.


Ultrastructural observation on the rectal gills of Zygoptera larva. The zygoptera larvae have tracheal gills. Both outer and inner epicuticles of damselfly larvae (Zygoptera) are pierced by pores of about 200...250 A thickness. The pores are distributed at a distance of about 500...600 A apart. Their endings are noticed upto the lower portion of exocuticle. The endocuticle is a big area in which the respiratory epithelium have flat apical and basal sides, plasma membrane is not folded. Glycogen granules are distributed in the cytoplasm and a few mitochondria are noticed. The tracheal brances and the cytoplasm of the respiratory epithelium come in contact with the epicuticle. The distribution of tracheoles, with surplus of tracheoles in a surface outside the hypodermis are close to the sub-cuticular area is found. The gills of Zygoptera appear to be merely respiratory organs having respiratory epithelium and the ions and water are resorbed from the modified rectal epithelium.


Studies on the toxic effect of cadmium in the rat. I. Testicular changes induced by a single subcutaneous injection of cadmium chloride. Adult male rats of Wistar strain were subcutaneously injected with a single dose of 0.025 mM CdCl2/kg body weight. The autopsies were performed 2 and 12 weeks post cadmium injection. Experimental animals showed a normal gain of body weight while a marked lowering of the testes weight was observed. The lowering of testes weight of cadmium treated rats occured mainly due to the necrosis of seminiferous tubules, volume of which is markedly lower if compared to intact control rats. After 2 weeks of experiment a marked enlargement of the interstitial gland of the testis occured while after 12 weeks the gland is markedly lower if compared to control rats and to rats studied 2 weeks post cadmium injection. Histochemical reactions for lactic, succinic, alpha-glycerophosphate and 3beta-hydroxy steroid dehydrogenases, NADH tetrazolium reductase, acid phosphatase and nonspecific esterases showed for irreversible necrotic changes of seminiferous tubules of cadmium treated rats while the damage to interstitial gland is only temporary.


Ultrastructure of cells of proper gastric glands and their stroma in hemorrhagic shock in the rat. Ultrastructural changes in the cells of the proper gastric glands and their stroma are biphasic in the hemorrhagic shock. First phase: "paralysis" of the capillary vessels with an oedema of their stroma, an intracellular oedema with hydropic degeneration of the parietal cells and a degranulation of the argentaffin and argentaffin-like cells. Second phase: ischemia of the gastric mucosa with ultrastructural features of: a) the increased secretion of the parietal cells, b) the degranulation of the chief gastric cells, c) the increased secretion processes in the mucous neck cells. These findings suggest that at last 2 factors coincide in the pathogenesis of so-called stress gastric ulcerations: biphasic disturbances of blood circulation initially damage the gastric mucosa and are followed the digestive hyperactivity of the gastric juice finally resulting stress ulceration.


Histomorphological and ultrastructural studies of a rat myocardium in experimental conditions. A histomorphological and ultrastructural analysis of the adaptation reaction of the rat heart muscle during an early experimental alloxan diabetes has been carried out. Changes were observed in the orthomorphology of the ultrastructure which appeared as vesicular intercalated discs within the fasciae adherentes regions, as changes in the mitochondria, cell nucleus, myofibrills and in the sarcoplasmic reticulum with a simultaneous increase in the number of lipid bodies. The described alterations are dependent on the duration time of the experiment and on the disturbances in the entire and local system cellular metabolism.


Investigations on the ultrastructure of spermatozoa in chronic alcoholics. The semen of 25 chronic alcoholics aged between 28 to 52 years was examined. The period of alcoholism amounted to 15 years the average being 12.3 years. In each person the semen was twice morphologically studied and the ultrastructure of spermatozoa was evaluated. Submicroscopic changes consisted mainly in the abnormal electron density of nuclear chromatin, altered structure of the acrosome and mitochondrial spiral, as well as in more rarely observed disturbed arrangement of fibrils of the axial filament complex. The hitherto non observed structure in human spermatozoon ön the form of single, annular, "whorls" were described. The interdependance between ultrastructural changes of spermatozoa and fertility was discussed.


A morphological study of antibody transport in the transparent fluid flowing form the lymph-folds of the copulatory organ into the cloacal lumen of the cock (Gallus domesticus). The lymph of the copulatory organ of the cock is produced mainly in the lymphobulbus phalli. The lymph fluid passes the secretory duct with its branching lymph capillaries and discharges into the lymph-fold. From here, it is released into the interstitial tissue through gaps of the lymph capillaries but also through the intercellular spaces between the endothelial cells of the lymph capillaries. A minor portion of the lymph is produced also in the lymph-fold from where it is transported in the interstitial tissue either by transfer vesicles of the circulatory blood capillaries or by pores and fenestrae of the transudatory blood capillaries. During the erection of the copulatory organ the lymph is pressed through the intercellular spaces of the epithelial covering the lymph-fold which is cylindrical and multistratified in shape. From here, it reaches the cloacal lumen in the form of transparent fluid (TF). TF was collected by different methods. It contains immunoglobulins which arise from plasma cells of the lymph-fold. Juxtaluminally, the lymph passage takes place via zonulae pseudooccludentes. The epithelial covering secretes a mucoid substance which is added to the TF prior to its following the spermatic phase as the final fraction of the ejaculate. Plasma cells and lymphocytes migrate along the lymph flow in the intercellular spaces of the epithelial covering of the lymph-fold. After an experimental application of antigen the sub- and intraepithelial cells and lymphocytes increase in number with their portion of active cells exceeding the less active ones. The increase coincides with the increase in antibodies detected in the TF where they may cause a local immunisation.


Hypertension due to renal infarction. Report of a case of successful treatment.


Traumatic rupture of the diaphragm.

Traumatic rupture of the diaphragm.


Congenital unilateral multicystic kidney.


Morphological changes related to absorption of undigested protein in small intestinal epithelium cells of suckling pigs.


Alkaline phosphatase, total protein, protein-bound hexose and hexosamine changes in serum and wound fluids in surgically-induced subcutaneous wounds in buffalo calves.


A comparative study of regression in Iranian schizophrenic and normal groups.


Large aneurysm of the splenic artery.

Large aneurysm of the splenic artery.


The abomasal digestion in calves during development of the forestomachs.


Bone mineral contents and serum alkaline phosphatase activity of healthy and hip dysplasic German sheepdogs.


Histological study of coccidiosis caused in the silver carp and the bighead by Eimeria sinensis Chen, 1956.


Effect of heat treatment, pelleting and storage on the content of thiamine and other vitamin B compounds of some fodders.


Alterations of the ruminal mucosa and its relation to the hepatic abscesses in bulls fed high energy and low fibre diets.


Use of the injection preparation Dertil (ad Us. Vet.) in fasciolosis control.


Abortive Capillaria hepatica infection in the guinea pig.


Evaluation of the local allergic (intrapalpebral) test for Aujeszky's disease virus infection in swine.


Droncit--a milestone in the control of Echinococcus granulosus in dogs.


Studies on avian mycoplasmas by metabolic inhibition test.


Influenza infectedness of captured and shot wild birds on north-eastern and south-eastern parts of Hungary.


Studies on heteroglucides in human colostrum. I. Isolation and characterization of a new sialic acid-containing hexasaccharide, disialyl-lacto-N-tetraose, from human colostrum.


Histochemical and electron microscopic observations on intraosseous ganglion.


Studies on heteroglucides in human colostrum. II. Isolation and characterization of five sialic acid-containing oligosaccharides from human colostrum.


Studies on heteroglucides in human colostrum. III. Isolation and characterization of a new fucose-containing sialooligosaccharide from human colostrum.


Studies on sialoglucides in human urine. II. On the qualitative and quantitative changes of urinary sialoglucides under various conditions and an attempt to identify sialoglucides in blood.


Two types of vestibulo-ocular reflex pattern caused by velocity and acceleration components of stimuli.


Studies on sialoglucides in human urine. I. Determination of sialoglucides with low molecular weights in human urine.


Effects of eyelid closure on vestibular nystagmus.


The comparative study of invertebrate glycosaminoglycans. II. Structural studies of succinean sulfate.


Electron microscopic observation on the outer hair cells and Deiters' cells of the rabbit.


The comparative study of invertebrate glycosaminoglycans. I. Isolation of a novel acidic glycosaminoglycan, succinean sulfate, from mucus of the sanil, Succinea lauta Gould.


Unique skin-sensitization to own plasma.


Hormonal changes after vasectomy.

Hormonal changes after vasectomy.


Exocrine hypoplasia of the pancreas--a case report.


Plasma creatinine and creatinine clearance studies in normal individuals.


Pattern of assault.

Pattern of assault.


Aetiological review of recurrent laryngeal nerve paralysis.


Prevalence and pattern of drug abuse among Delhi University College students.


Piperazine neurotoxicity and psychological reaction.


Pulmonary candidiasis.

Pulmonary candidiasis.


Fracture of vertebral bodies due to accidental electric shock.


Angiolipoma of the labia majora.

Angiolipoma of the labia majora.


Histoplasmosis.

Histoplasmosis.


Role of IMA in nation building.

Role of IMA in nation building.


Herpes zoster-transverse myelitis.

Herpes zoster-transverse myelitis.


The association of leprosy and tuberculosis.


Enzyme pattern in vascular diseases of brain.


Burns:.

Burns:.


Lymphomatoid papulosis. A model of immunoblastic reaction of the skin. In 3 cases of lymphomatoid papulosis with few ulceronecrotic lesions and 5 with the classic features of pytiriasis lichenoides acuta with necrotic lesions, a considerable part of the cellular component of the infiltrate of the lesions showed the morphologic and cytochemical characteristics of the cells described as immunoblasts. The authors consider that the lymphomatoid papulosis could represent an immunoblastic response to an unknown antigen.


Congenital disseminate organoid tumors.

Congenital disseminate organoid tumors.


Organoid nevus.

Organoid nevus. The organoid nevus is a malformation of most of the normal tissue components in a strictly localized, smaller or larger area of skin, most commonly the scalp. It undergoes more or less predictable developmental changes in puberty and then remains stable, but is subject to secondary development of benign or semimalignant adnexal tumors in a significant percentage of cases. It may present clinically and histologically in a variety of forms, the most common ones being nevus sebaceus and nevus verrucosus. It may be associated with neurologic and skeletal abnormalities in the organoid nevus syndrome (Schimmelpenning-Feuerstein-Mims). Total excision before adolescence is the treatment of choice.


Collodion baby.

Collodion baby. The study of 198 collodion babies, 29 personal cases and 169 from the literature was undertaken. The collodion membrane with spontaneously desquamate between the 15th day and 3rd month of life. During the neonatal period one third of the infants die due to pulmonary complications or infection. The collodion membrane seems to have an increased permeability. The desquamation is followed by a relapse which leads to different keratinization disorders, most frequently nonbullous congenital ichthyosiform erythroderma.


Toxic epidermal necrolysis - a review.

Toxic epidermal necrolysis - a review.


Pediatric dermatology: internal and external medicine.


Familial annular erythema.

Familial annular erythema.


Early signs of leprosy in children.

Early signs of leprosy in children. Leprosy in children is more frequent than generally thought. Diagnosis is not easily made because early signs of the diseases are overlooked. Statistic data from the Dermatologic Clinic Pascua of Mexico City reveal that early signs are cutaneous in 67%  of cases: hypochromic and anesthetic infiltration, nodules, and alopecia of eyebrows and eyelashes. Neurological symptoms such as anesthesis, facial paralysis, thickening of nerves and trophic problems are present in 19.3% of cases. The nose mucosae are congested in 9.2% of cases. Systemic manifestations may be present in 4% of cases. Examination of contacts living with lepromatous patients is stressed as the best way to discover the early signs of the disease. Dermatological consultation in  general and pediatric hospitals is also recommended. If diagnosis is delayed, leprosy can induce physical, psychological and social disabilitation of the patient.


Urinary esterase activity of patients with atopic dermatitis and effects of t-AMCHA on it.


Lipodystrophia centrifugalis abdominalis infantilis.


Progeria in childhood with description of a new progeroid syndrome displaying gross Fabry-like changes in heart, great vessels and kidneys.


Hemangiomas in the epidermal nevus syndrome.


Congenital and non-congenital depigmentation. The hypofunction of melanocytes results in congenital and noncongenital depigmentation. Congenital depigmentation can be further divided into two groups; the first is depigmentation caused by loss of melanization in premelanosomes represented by oculocutaneous albinism; the second group is congenital decrease of melanocytes represented by nevus dipigmentosus. In contrast, acquired progressive depigmentation represented by vitiligo vulgaris and Sutton's leukoderma is characterized by the loss of premelanosome synthesis. The pathogenesis of these three major types of melanogenic defects has been further discussed with the current clinical, biological and immunological view of melanogenesis.


Disseminate pigmented nevi and short stature.


Giant cavernous haemangiomata.

Giant cavernous haemangiomata.


The skin and the heart in inherited disease.


HLA antigens in atopic diseases.

HLA antigens in atopic diseases. The immunogenetical relationship among atopic diseases was investigated from the point of human major histocompatibility antigen. The relationship between IgE level and HLA antigen was also discussed. (1) Atopic dermatitis had no high frequency in HLA antigens. HLA-B12 in bronchial asthma and HLA-BW40 in allergic rhinitis were frequently found in the population study. (2) No HLA-BW15 was observed in these three diseases. The term called 'disease-resistant antigen' was proposed in this paper. (3) We considered that IgE level is not controled by HLA antigen.


Light-sensitive dermatoses in children.

Light-sensitive dermatoses in children.


Contribution of the H reflex to the study of spasticity in adolescents.


Physiotherapy in obstructive airways diseases.


Blood gases, rational oxygen therapy, air flow limitations.


Anatomy of the lung and patterns of structural change in disease.


Education and training in the remedial professions.


Thermoplastic collar survey.

Thermoplastic collar survey.


Sports medicine and the physiotherapist.


St James' Hospital NHS Sports Medicine Clinic.


Physiotherapists in sport: reflections from America.


Sports medicine in school.

Sports medicine in school.


Testing for fitness.

Testing for fitness.


Post-operative management of chronic achilles tendon pain in sportsmen.


The combined effect of propranolol with bethanidine or methyldopa in hypertension.


Studies with a new cardioselective beta-blocker, metoprolol. These studies indicate that the cardioselective beta-adrenergic blocking drug metoprolol might be expected to be at least as effective for the treatment of arterial hypertension as propranolol. The difference in effect on the peripheral vascular bed may be of importance when acute increase of the blood adrenaline level occur, as in severe anxiety states. Experience in long-term studies in all kinds of patients with raised arterial pressure has confirmed that metoprolol is an active antihypertensive agent of similar potency to other beta-blocking agents.


Dissociation of the renin lowering and antihypertensive actions of propranolol. Suppression of renin release by beta-adrenoceptor blocking drugs has been advocated as the prime blood pressure lowering mechanism of these agents. The present report casts doubt on this proposition in three ways. In the first study, involving 22 patients with borderline hypertension, patients with elevated plasma renin levels showed normalization of the blood pressure after pharmacological autonomic blockade with intravenous atropine, propranolol and phentolamine, the time course of the pressure fall being such as to exclude suppression of renin release as the antihypertensive mechanism. It is clear that in patients with mild hypertension and high plasma renin levels, the elevation of blood pressure is maintained by a neurogenic mechanism, the elevated plasma renin having no direct role in sustaining the higher blood pressure. In two additional studies involving 21 patients in all with mild to moderately severe essential hypertension, to whom propranolol was administereed by mouth for 1--3 months, two findings were of interest:1) patients with low-renin essential hypertension showed a good antihypertensive response to propranolol, and 2) in some patients a dosedependent dissociation of the renin and blood pressure lowering action of propranolol could be demonstrated. These findings militate against the assumption that propranolol selectively lowers the blood pressure in "renin-dependent" hypertension, and strongly suggest that the antihypertensive action of this drug is not mediated by depression of plasma renin activity.


The effect of prindolol on plasma noradrenaline, plasma renin and sodium-potassium metabolism in patients with essential hypertension. The chronic administration of prindolol in patients with essential hypertension resulted in the following: 1) a significant decrease in blood pressure and heart rate (with only the exception of the unchanged diastolic blood pressure after the stimulus of the seven-minute standing period), 2) a significant decrease of plasma noradrenaline concentrativn at rest and under orthostatic conditions, 3) a significant decrease of PRC at rest and an even more pronounced suppression of PRA after the stimulus of upright posture, 4)a significant decrease in total exchangeable sodium and 5) a concomitant significant increase in total body potassium even with an increase in body weight. These findings are not subject to easy interpretation. In particular, we cannot conclude which of the changed parameters plays the initial role in lowering blood pressure. A working hypothesis might presume that beta blocking agents inhibit central and/or peripheral sympathetic nervous activity. The results reduction in plasma renin concentration would in turn lead to a drop in aldosterone secretion rate indicated by the increase in potassium and decrease in sodiummour data would support such a sequence of events.


A new method for rapid identification of influenza virus isolates. With the use of bacteria sensitized by influenza virus strain-specific antisera, virus isolates can be identified rapidly. One drop of virus suspension is mixed with one drop of sensitized bacteria on a slide that is then agitated; reaction occurs within 10 minutes. The test is subtype-specific. The mehod is based on the fact that the cell wall of the Cowan type 1 strain of Staphylococcus aureus contains abundant quantities of an antigen, known as protein A, that reacts with the IgG molecule by binding it in such a manner that the antibody-combining sites remain free. If an antigen homologous to the antibody coated on the surface of the bacteria is added to the suspension of sensitized staphylococci, agglutination occurs.


Management of maple syrup urine disease in Canada. Committee for improvement of Hereditary Disease Management. Nine patients with classic maple syrup urine disease (MSUD) and four with variant forms are under care at five treatment centres in the network affiliated with the National Food Distribution Centre for the Treatment of Hereditary Metabolic Diseases (the "Food Bank"). Diagnosis was made by clinicians and not from mass screening programs. MSUD requires complex emergency treatment to prevent severe neurologic damage, but effective management is compatible with normal growth and development. Long-term treatment requires continuous monitoring of the response to diets restricted in branched-chain amino acids; semisynthetic diet products free of branched-chain amino acids, provided by the Food Bank, are essential. Centralized treatment programs reduce the cost of treatment and maximize the potential benefits. The leucine requirement for adequate somatic growth during infancy in MSUD was found to be 200 to 600 mg/d; this range is lower than that estimated for infants with an intact leucine catabolic outflow pathway. The requirements for isoleucine and valine in infancy were also found to be lower than published values for normal infants.


Echocardiographic features of an unusual case of aortic valve endocarditis. In a patient with aortic valve endocarditis a myocardial abscess, complete heart block and acute aortic regurgitation developed. Echocardiography gave evidence of large aortic valve vegetations, and at operation vegetations were found to have destroyed the right coronary cusp and part of the noncoronary cusp. Following surgery the patient recovered. Echocardiography may prove to be a useful noninvasive technique to aid in the timing of surgical therapy in patients with valvular vegetations.


Failure of acetylmethadol in treatment of narcotic addicts due to nonpharmacologic factors. Acetylmethadol, a new narcotic substitute, has a longer duration of action than methadone. Seventeen subjects, former heroin users currently under methadone treatment, entered a study of the toxicity and efficacy of this drug. Only nine subjects completed the assessment phase of the study and began the acetylmethadol phase, and only one completed the 8-week study phase. Hence, no conclusions can be drawn about acetylmethadol's efficacy. The high attrition rate was unrelated to pharmacologic factors; the subjects were concerned that if this drug was effective there would be no methadone to take home and hence no opportunity to trade, sell or "play with" (that is, combine with other drugs) the latter. This study emphasizes the difficulty in determining the efficacy of specific drug treatments for opiate-dependent patients.


The golden years: CMA senior members look at the joys and problems of retirement.


So you saved in an RRSP; how about spending it?


Hereditary metabolic disease in Canada.

Hereditary metabolic disease in Canada.


The swine influenza vaccination program.


The child with recurrent abdominal pain.


Treatment of diabetes in psychogeriatric patients.


Health manpower statistics.

Health manpower statistics.


Taeniasis in southern Alberta, 1894-1900.


Physician manpower requirements.

Physician manpower requirements.


Practical guidelines for assessing patients positive for hepatitis B surface antigen. In assessing the patient the hepatitis B surface antigen (HBsAg) the physician must decide on the basis of physical findings, results of laboratory tests and biopsy, when indicated, whether the patient is an asymptomatic carrier or has acute or chronic hepatitis. Asymptomatic carriers of HBsAg must be educated in personal hygiene and the possibility of transmission, should not be allowed to donate blood or breast-feed and should not work with blood products for human use or pharmaceutical products designated for intravenous use. However, it is otherwise not necessary to advise these individuals to change their profession.


Feasibility of routine testing for hepatitis B surface antigen in hospital employees and restriction of carriers. In 1972-73, 48 hospital staff members were tested selectively for hepatitis B surface antigen (HBsAg); 4 (8.3%) were found to be HBsAg-positive. In 1974-75, 1415 staff members were tested routinely before employment and at periodic health examination; 25 (1.8%) were found to be HBsAg-positive. Of the HBsAg-positive staff members 55.2% were Asians, this proportion being significantly (P less than 0.05) greater than that of any other ethnic group, and 31.0% were southern Europeans. Nurses and laboratory technologists were the largest professional groups among the HBsAg-positive staff, each accounting for 20.7%. Our results indicate that it is impractical to carry out routine testing of hospital staff for HBsAg. Selective testing and restriction from work in their units is proposed for staff of the renal and peritoneal dialysis units, the emergency department and the intravenous team and dietary staff who handle food directly.


Detection of core antibody in hepatitis B infection. Hepatitis B core antigen (HBcAg) is found on the decoated Dane particle and on a morphologically similar particle detected mainly in the nucleus of hepatocytes of patients with hepatitis B. HBcAg prepared from the liver of a chimpanzee infected with hepatitis B virus was used to test human serum for core antibody (anti-HBc) by complement fixation. Anti-HBc was found in serum collected from patients with hepatitis B in both the acute and convalescent stages, from carriers of hepatitis B surface antigen (HBsAg) and from patients with chronic liver or renal disease who were carriers of HBsAg. It was not found in patients with hepatitis A or infectious mononucleosis, or in healthy persons who were not carriers of HBsAg.


Teratoma of the ligamentum teres of the liver--a case report.


A case of sideroblastic anemia.

A case of sideroblastic anemia.


Pituitary and adrenal influences upon spontaneous contractions of uterine horns isolated from ovariectomized or natural estrus rats.


Ophthalmic angiography of malignant exophthalmos.


A right subclavian artery as the last branch of the aortic arch.


Incidence of peripheral arterial disease in patients of ischaemic heart disease in India.


Preliminary report on electric evaluation of implanted pacemaker impulse by high speed oscilloscope.


Perhexiline maleate in angina pectoris.

Perhexiline maleate in angina pectoris.


Oxidative and glicolytic metabolism of the frontal cortex (latero-frontal) and of the posterior cortex (latero-occipital) in relation with the sexual activity of the rat. The authors of this paper have ascertained the glycolytic metabolism and the oxidative metabolism (intake of QO2), of the frontal and posterior cortex in female rats at different stages of the sexual cycle, as also in ovariectomized animals, by the intake of glucose and the production of lactates. The results indicate a statistically significant increase of the oxidative metabolism of the posterior cortex (latero-occipital) in the estrual and proestrual phases, in comparisons with the diestral phase. The frontal cortex (latero-frontal) did not show any significant difference; moreover, the glycolitic metabolism did not alter in any of the tissues under observation. These findings, seem to suggest possible participation of the posterior cortex (latero-occipital) on the regulation of sexual cycle of the rat. The activation of this cortex occurs through the preponderant imbricantion of the tri-carboxylic acid cycle.


Primary drug resistance in pulmonary tuberculosis.


Therapeutic problems in the management of giant-sized mediastinal tumours.


Serum luteinizing hormone follicle-stimulating hormone and prolactin in neonatal-androgenized rats. Serum levels of LH, FSH, Prolactin and Testosterone of 90 days old male rats androgenized soon after birth were determined by specific radioimmunoassay and were compared to untreated rats. LH and FSH levels were also determined in 90 days old female rats neo-natally treated with testosterone and compared with normal diestrus rats. Androgenization of male rats significantly increased serum FSH and Prolactin levels without producing changes in plasma LH and testosterone concentrations. Similar increase in the FSH levels were found in androgenized female rats although plasma FSH concentrations were lower than in the male groups. These results obtained in male rats give an additional evidence that androgens acting in the first days of life are responsible of the higher levels of FSH and Prolactin that characterize the male or tonic pattern of gonadotrophin secretion.


Percutaneous needle biopsy of the lung: diagnostic cogitability and inherent changes.


Benign superior vena caval obstruction.

Benign superior vena caval obstruction.


Primary mesothelioma of the pericardium.


Gastric allergy to rifampicin.

Gastric allergy to rifampicin.


Human chorionic somatommamotropin (HCS) and pregnancy. Its relation with insulin. Plasma HCS levels have been measured in normal and pathological pregnant women. In the normal group HCS levels increased from 6--8 weeks till 33-34 weeks and then felt significantly. HCS pattern in prediabetic and chemical diabetic pregnant women was similar to the normal group. However HCS levels in chemical diabetics were significantly higher during the first two trimesters. HCS levels increased in twin pregnancy, diminished in cases of eclampsia, hypertension, fetal growth retardation, mole and blighted ovum, and disappeared after intrauterine death. Nothing could be deduced from the obese and Rh-isoimmunization groups. It is confirmed the value of HCS determination as an index of placental maturation. Also, insulin/HCS ratio may be of some aid in the study of carbohydrate intolerance in pregnancy.


Metronidazole in tropical eosinophillia: a phase II trial.


The urban malaria problem in Tamilnadu State.


Electrocardiographic changes in cerebrovascular accidents.


Glycosaminoglycan content of normal human neonatal aorta.


Surveiliance of intestinal amoebiasis and its treatment over a period of 3 years with metronidazole in an institution.


Routine management of acute myocardial infarction.


A study of serum iron and total iron binding capacity in hepatic cirrhosis.


Iodide myxoedema simulating filariasis.

Iodide myxoedema simulating filariasis.


Thiamine absorption in the rat. IV. Effects of caffeic acid (3,4-dihydroxycinnamic acid) upon absorption and active transport of thiamine. The effects upon thiamine absorption in-vitro and in-vivo by caffeic acid (a thiamine antagonist isolated from bracken) was studied, partly using 14C-thiamine. It was again shown that caffeic acid reduced the quantity of thiochrome positive thiamine, dependant upon the concentration ratio caffeic acid/thiamine. Caffeic acid was able to pass across the intestinal wall and to exert its antithiamine effect in the serosal incubation fluid. When caffeic acid was present in the mucosal fluid the amount of thiochrome positive thiamine passed to the serosal side was diminished according to the mucosal caffeic acid concentration. In-vitro studies with 14C-thiamine revealed, however, that thiamine modified and turned into a thiochrome negative form by caffeic acid was absorbed similarly to unaffected thiamine. Active transport in-vitro of thiamine was significantly inhibited by the presence of caffeic acid.


Antithiamine effect of coffee.

Antithiamine effect of coffee.


Enzymatic microassay of pyridoxal-5'-phosphate using L-tyrosine apodecarboxylase and L-(1-14C) tyrosine. A microassay for pyridoxal-5'-phosphate (PLP) is described, which incorporates both the enzymatic and radioisotopic techniques. The method is based upon the stoichiometric decarboxylation of L-(1-14C) tyrosine by L-tyrosine apodecarboxylase and PLP. This method is an extensive modification of the ones reported by us previously, the main features of this present method being the ease of preparation of the enzyme extract and the substrate, elimination of the preincubation period and shortening of the 14CO2-trapping step. The assay as described here is rapid, highly sensitive and specific.


Isolation, partial characterisation of antithiamine factor present in rice-bran and its effect on TPP-transketolase system and Staphylococcus aureus. 1. A light yellow viscous oily antithiamine compound designated as Compound X was isolated from rice-bran and it was composed of three components--glucose, a yellow viscous oily substance (Fraction A) and a reddish brown amorphous substance (Fraction B). 2. 1 mg of Compound X inactivated 3.5 microgram of thiamine hydrochloride whereas 1 mg of Fraction A inactivated 26.5 microgram of thiamine hydrochloride. Fraction B had no antithiamine activity. 3. Fraction A was partially characterised as benzene nucleus with ortho dihydroxyl groups, one methyl group and a side chain containing hydroxyl and carboxylic acid groups. 4. Compound X as well as Fraction A inhibited the utilisation of ribose-5-phosphate by TPP-TK system of human erythrocyte hemolysate and the growth of Staphylococcus aureus was retarded by these compounds.


Erythrocyte glutathione metabolism in cobalt-deficient goats. Cobalt deficiency was produced in goats by feeding them rhode grass hay. The deficient animals excreted increased amounts of methyl malonic acid in their urine, indicating a lack of vitamin B12. Erythrocyte reduced glutathione levels increased with the onset of anemia. There was a concomitant increase in the levels of erythrocyte glutathione reductase (GSSG NADPH Reductase) and glutathione peroxidase (GSH:H2O2 peroxidase)during deficiency. These results are compared with similar observations reported for vitamin B12 deficiency in humans.


Ascorbic acid synthesis in certain guinea pigs. Three guinea pigs fed a vitamin C-free diet manifested no symptoms of scurvy even after 4-8 months, normally increased in body weight and excreted quantities of ascorbic acid in urine far exceeding the total body pool of ascorbic acid. The course of healing subsequent to experimental trauma in one of these animals proved to be entirely normal and vitamin C concentration in its liver after 8 months of a scorbutogenic regimen was found to be more than twice that in guinea pigs with a daily intake of 10 mg ascorbic acid. It is evident that certain guinea pigs are capable to synthesize ascorbic acid that fully covers the needs of the organism. However, the freqency of occurence of such guinea pigs appears to be extremely small.


In vivo acylation of triglycerides and phosphoglycerides of liver subcellular fractions with palmitate-1-14C in rats fed vitamin A. Incorporation of palmitate-1-14C into liver and its mitochondrial, and microsomal triglycerides and phosphoglycerides of rats fed 100000 I.U. of vitamin A for two days has been studied. Feeding of vitamin A significantly increased triglycerides in liver and its mitochondrial, microsomal and supernatant fractions as compared to the controls. A significant increase in total phospholipids and phosphatidyl choline was noted in liver mitochondrial and microsomal fractions of vitamin A fed rats. The incorporation of palmitate-1-14C (counts/min/liver) into triglycerides of liver mitochondrial, microsomal and supernatant fractions of vitamin A fed rats was significantly higher, whereas in phosphatidyl ethanolamine and phosphatidyl choline it was not significantly different from the controls.


Effect of vitamin E deficiency on the level of superoxide dismutase, glutathione peroxidase, catalase and lipid peroxide in rat liver. The activities of superoxide dismutase, glutathione peroxidase, catalase and xanthine oxidase were simultaneously studied in vitamin-E deficient and -supplemented rat liver and also measured the lipid peroxide content in liver. The lipid peroxide content of vitamin E-deficient rat liver, estimated by thiobarbituric acid, increased as compared with that of vitamin E-supplemented rat liver. No marked changes of activities of superoxide dismutase, glutathione peroxidase and catalase were observed, but the activity of xanthine oxidase which is strong superoxide generator increased in vitamin E-deficient rat liver. These results suggest that vitamin E prevents the accumulation of lipid peroxide, but not controls the level of peroxide scavenging system such as superoxide dismutase, glutathione peroxidase and catalase.


Studies on the intestinal hydrolysis of tocopheryl esters.


Vitamin E tolerance test in various gastrointestinal disorders.


Dose - response relationship between warfarin and the metabolism of phylloquinone (vitamin K1) in man.


Vitamin A and congenital malformations.

Vitamin A and congenital malformations.