Metabolic behaviour of water-soluble fluorescent whitening agents in the rat and bean plant. The behaviour of three representative water-soluble fluorescent whitening agents (FWAs) was studied in rats and bean plants using 14C-labelled compounds. Following oral doses of 5 mg/kg of each of the FWAs to rats, rapid and complete excretion of radioactive material was observed, with an excretion half life ranging from 7-13 hours. Faeces were practically the only route of excretion, indicating, in combination with the short half life times, that no significant amounts of whitener were absorbed from the digestive tract. No radioactive residues were found in blood, liver, kidney, brain, muscle, or fat 96 hours after dosing (limit of quantitative determination 0.005-0.01 ppm Fwa equivalents). Whereas, after application of compound (1) and compound (3), radioactive material was completely extractable from faeces in the form of unchanged parent compounds, that in the compound (2) experiments was not extractable. When bean plants (Phaseolus vulgaris) were grown in the presence of 14C-labelled compound (3) in nutrient solution (11.5 ppm) and in soil (,7.5 ppm), approximately 55% and 2%  of the applied radioactive material, respectively, was absorbed by the plants. More than 90% of thie material was bound to the roots in both experiments 40 days after the application. Eight percent of the radioactive material absorbed in the soil experiment was translocated mainly into the stems whereas only trace amounts of the order of less than 0.1 ppm reached the leaves and bean pods. It is concluded from these experiments with extremely high concentrations of compound (3) that under the conditions which may occur in practice residues of water-soluble FWAs do not appear in substantial amounts in edible parts of crops.


Mechanism of whitening and quenching.

Mechanism of whitening and quenching. The processes of radiation absorption in a molecule and the energy release by thermal or chemical deactivation, phosphorescence, and fluorescence are outlined briefly, and the difference between fluorescent and non-fluorescent dyestuffs is discussed under physical aspects. After a description of the optical mode of action of fluorescent whitening agents (FWAs), the colorimetrical methods of characterizing FWAs and evaluating the whiteness are investigated.


Acute oral, dermal, and inhalation studies. By appropriate testing procedures, it has been demonstrated that the compounds used as fluorescent whitening agents (FWAs) have a very low order of systemic toxicity in acute experiments. No evidence of dermal toxicity has been found. The full chemical names of the fluorescent whitening agents (FWAs) investigated in this study are listed below. They have been numbered for easy identification and classified by chemical groups.


Study by fluorescence microscopy of the effect of fluorescent whitening agents on the skin of mice. Fluorescence microscopic studies of the skin of hairless mice showed that a fluorescent whitening agent (FWA) of the bis(phenyltriazolyl)stilbenedisulfonate type did not penetrate into the subepithelial layers (dermis and subcutaneous tissue) of the skin after cutaneous application.


Toxicological investigations with fluorescent whitening agents. The reasons for which toxicological investigations are carried out are: -handling and consumer safety, -the critical attitude of the public towards the use of chemicals in general, -the need to weigh benefit against risk when using chemicals, and -the safety requirements introduced by authorities. The guidelines for toxicological evaluation of chemicals and particularly fluorescent whitening agents (FWAs), as well as the extent of which man is possibly exposed to these substances through alimentary intake or through contact with the skin are discussed. It is concluded that there is a very wide safety margin for every conceivable use of FWAs.


Chronic toxicity and carcinogenicity studies with FWAs. Compounds (1), (2), (3), and (4) caused no adverse toxic effects and yielded no evidence of carcinogenic activity in a 2-year feeding study in albino rats at dietary levels up to 1000 ppm. Compounds (2), (3) and (4) were without effect in a 2-year study in beagle dogs at levels up to 2000 ppm. Compound (1) at levels of 400 and 1000 ppm led to decreased weight gains in some dogs and caused doserelated pathology consisting of inflammatory changes on the serosal surfaces of abdominal viscera and increased hematopoietic acitivty of the spleen and liver cords. These effect could have been due to the feeding of material, without adjustment of pH, for the first 30 weeks of the study.


Studies on the reaction of skin when exposed to fluorescent whitening agents. In hand washing tests with detergents containing fluorescent whitening agents (FWAs), the amounts of whitener left on both hands were determined by TLC spectrophotometry: they varied from 0.06 mg to 0.17 mg. Whiteners of different chemical constitutions behaved in a very similar manner. After 24 hours the skin of the treated hands was virtually free of whitener.


Photocarcinogenesis: lack of enhancement by fluorescent white agents. These studies were designed to determine whether fluorescent whitening agents (FWAs) could: (I) produce an augmented acute response of skin to a single ultraviolet light exposure (i.e., phototoxicity), or (II) increase the number or hasten the appearance of skin tumors after several ultraviolet light exposures (i.e., chemically enhanced photocarcinogenesis). Five substituted stilbene FWAs were screened for phototoxicity. The results of pretreatment with these agents were compared with pretreatment by a known photoxic agent, 8-methoxypsoralen (8-MOP) or the vehicle (methanol) using the skin of hairless mice, miniature pigs, and man. None of the FWAs was phototoxic. Photocarcinogenesis testing involved pretreating hairless mouse skin with FWAs, 8-MOP, or methanol only before each daily exposure to simulated solar ultraviolet light. In terms of tumor yield and tumor development time, photocarcinogenesis was enhanced by 8-MOP, but not by FWAs.


Studies of embryo toxicity in rats and rabbits. Embryo toxicity studies were carried out to investigate the effects of different doses of fluorescent whitening agents (FWAs) after oral administration to pregnant rats from the 6th to the 15th day of pregnancy and to rabbits from the 6th to the 18the day of pregnancy. In own studies two FWAs of the bis(triazinylamino or triazoly)stilbenedisulfonic acid type showed no embryo-toxic or teratogenic effect on wither species at daily doses up to 1000 mg/kg. Also the toxic dose to the mothers, 3000 mg/kg, given to rabbits in case of one FWA was without these effects. In the studies of KEPLINGER, et al. (Toxicol. Appl. Parmacol. 27: 494-506, 1974) a triazolylstilbenemonosulfonic acid derivative, two bis(triazinylamino)stilbenedisulfonic acid derivatives and a bis(sulfostyryl)diphenyl derivative had no terato-enic effect on rabbits when administered orally in doses of up to 30 mg/kg/day.


Three-generation Reproduction studies with FWAs. Four fluorescent whitening agents (FWAs), compounds (1), (2), (3) and (4) (see table 1) were investigated for their effects on the reproductive performance of albino rats through 3 successive 2-litter generations at dietary levels of 40, 200, and 1000 ppm. Except for slight and random effects of compound (4) on pup survival, no adverse effects were observed. The random effects with compound (4) were not consistently related to either dose level or cumulative duration of exposure through the 3 generations.


Testing mutagenic properties with the dominant lethal test on the male mouse. Mytagecity studies were carried out with fluorescent whitening agents (FWAs) using the dominant lethal test on male mice. Own tests with five FWAs, and those of KEPLINGER, et al. (Toxicol. Appl. Pharmacol.27: 494-506, 1974), with four FWAs, are described. In our tests, acute oral administration of five FWAs at a dose of 5000 mg/kg body weight gave no evidence of a mutagenic effect during 8 weeks' mating. The FWAs used were three bis(triazinylamino)stilbenedisulfonic acid derivatives and a 1,3-diphenyl-2-pyrazoline derivative. The results of the tests carried out by KEPLINGER, et al. showed that intraperitoneal injection of the four FWAs produced no mutagenic effect during six weeks' mating; the whiteners used were a triazolylstilbenemonosulfonic acid derivative (50 mg/kg), two bis(triazinylamino)stilbenedisulfonic acid derivatives (50 mg/kg) and a bis(sulfostyryl)biphenyl derivative (10 mg/kg).


Nucleus anomaly test and chromosomal analysis of bone marrow cells of the Chinese hamster and dominant lethal test in male mice after treatment with fluorescent whitening agents. Four fluorescent whitening agents (FWAs) were tested for mutagenic activity after oral administration in the following three different mammalian test systems: (1) Dominant lethal test in the male mouse; (2) Cytogenetic studies on metaphase chromosomes from the bone marrow of the Chinese hamster; (3) Nucleus anomaly test in somatic interphase cells (bone marrow) of the Chinese hamster. These investigations yielded no evidence of dominant lethal effects of any of the four compounds on the progeny of male mice. Furthermore, the data obtained from chromosome analyses and the nucleus anomaly test revealed no effects at all. Thus, the results obtained in all these tests gave no indication of mutagenic activity after the administration of these compounds in dosages up to approximately 1/3 or the LD50.


Legal aspects.

Legal aspects. The manufacture, application, use and disposal of fluorescent whitening agents (FWAs) may give rise to legal questions relating mainly to environmental protection and the effects on man and animals. In addition to legal aspects, certain commercial aspects such as the law of competition and the obligations of industry, including compensation for damage caused by FWAs, are discussed.


Technical aspects of whitening.

Technical aspects of whitening. The applicational and ecological advantages of whitening over conventional bleaching processes in the textile and paper industries are outlined.


Measurement of fluorescent white effects and whiteness. This report surveys the literature and describes various techniques of whiteness measurement and evaluation in current use. Measuring techniques are described for dealing separately with the effects obtained by bleaching, blueing and fluorescent whitening, and an example is given of the direct quantitative estimation of a fluorescent whitening agents (FWAs) on a substrate by measuring reflectance in the ultraviolet region. Another chapter deals with the colorimetric estimation of the whiteness and the shade of a fluorescent white using modern apparatus in conjunction with a programmable minicomputer. A new simple and universally applicab,e formula was worked out: W=D-Y+P-x+Q-y+C which has been successfully used in routine tests and which for the first time gives different weight to whiteness values corresponding to all shade preferences existing in theory. Each user can match the formula to his own preference by appropriate adjustment of the D, P, Q andC values. Y,x and y are the customary colorimetric values as standardized by the CIE (Commission Internationale de l'Eclairage). It was also found that with another formula the shades of fluorescent whitening effects (green to red tints) may be defined in a simple way.


Mass whitening of synthetic fibers and plastics. The evaluation of new and improved techniques has led to the development of mass whitening methods for use with synthetic fibers and plastics. The processes are complex but well defined, and call for the use of sophisticated fluorescent whitening agents (FWAs) to meet the requirements of the fiber or plastics manufacturer, the textile finisher, and the end user. The performances of some leading commercial whiteners are compared and their interactions with dyes, fillers and stabilizers are discussed.


Whitening of textiles.

Whitening of textiles. In the textile field, fluorescent whitening agents (FWAs) can be used on practically all types of goods at any stage in the finishing process. As examples, three important types of methods for applying FWAs to textiles have been outlined.


Fluorescent whitening agents in detergents. Washing is a form of textile care which is characterized by its repetitive nature. Washing methods vary enormously in different parts of the world. The main types of detergents and fluorescent whitening agents (FWAs) are described. Washing slows down the deterioration in use of white goods, and yellowing is counteracted by FWAs. FWAs also enhance the freshness and brightness of most pale shades. Cost calculations show clearly the economic advantages of using FWAs in washing: the useful life of textiles can be prolonged considerably for a very small additional cost.


Qualitative thin layer chromatography of fluorescent whitening agents. Experimental details and examples of a "chromatographic program" for testing the major fluorescent whitening agent (FWA) types and material samples containing FWAs are described.


"Chronic myelogenous" leukemia of juvenile type. Report of two cases and review of therapy. The juvenile type of "chronic myelogenous" leukemia (CMLJT) is a rare disease with only 40 cases reported to date. Clearly distinguishable from adult CML on both clinical and laboratory grounds, is is often confused with "congenital" leukemia, pseudoleukemia, leukemoid reactions or chronic granulomatous disease. According to studies of muramidasuria and colony-forming cells it is neither a chronic nor a granulocytic leukemia. It is a panmyelopathy with monocyte predominance and should thus be classified as a variant of myelo-monocytic leukemia. We review reported responses to chemotherapy and splenectomy and report our results with cytosine arabinoside in the treatment of 2 cases with this disease. Chemotherapy may prolong life and splenectomy may be useful in some cases; but the survival rate is 0%, justifying new approaches.


Plasmids: introductory remarks.

Plasmids: introductory remarks.


Differences in chromatin proteins of resting and growing human lymphocytes.


Spontaneous pneumothorax as a complication of pulmonary metastatic sarcoma. A report of three cases.


"Fluoride: ten-year prospective study of deciduous and permanent dentition"--a critique.


Nutritional determinants of the ecology of the oral flora. Consideration of nutritional factors as determinants of oral microbial ecology leads to the conclusion that endogenous factors play a dominant role. It is the interactions between specific microorganisms and nutritional and other factors provided by the host that selectively determine the kinds of microorganisms which willinitially reside in the oral cavity and the sites which they will colonize. The persistence of these original "colonists" in their respective ecologic niches will depend in part on the accessibility of endogenous nutrients variously provided by saliva, tissue exudates, crevicular fluids, or degenerating host cells. It will also depend on their response to and interaction with microorganisms which immigrate to that site subsequently. The microbial ecology of a given site is therefore rarely uncomplicated, the notable exception occurring at the most microscopic level where one may encounter microcolonies of individual species within more heterogenous populations of microorganisms. The extent of this heterogeneity quantitatively and qualitatively is a reflection of the degree to which the interactions between the resident flora and the new arrivals, plus the local environmental changes which they both generate, serve to promote or to discourage the survival and growth of the individual species. This element of heterogeneity in a dynamic system with its inherent potential for variation makes it possible for relatively minor changes in local environmental conditions to result in significant differences in the microflora between one site and another even though they may be in close proximity to each other. Once this concept is appreciated it becomes easy to understand how disease processes such as dental caries may affect specific areas of a tooth while nearby areas are unaffected. The effects of exogenous nutrients on the ecology of the oral microbiota, nevertheless cannot be ignored. The diet may modulate such endogenous factors as the salivary secretions and the local resistance of the gingival tissues to infections. Although at our present state of knowledge the direct influence of dietary proteins and fats on the oral microbiota is thought to be of relatively minor consequence, dietary carbohydrates are of major ecological significance. Dietary sugars provide readily available substrates for the oral microorganisms, most of which depend on carbohydrates for energy sources. The metabolism of dietary sucrose by S. sanguis and S. mutans with the productions of acids and intracellular and extracellular polysaccharides has specific influence on the microbial composition, metabolic activities, and mass of coronal plaque. The ready availability of dietary carbohydrates undoubtedly influences the microflora of other parts of the oral cavity as well, eic species or indirectly through the interactions of other organisms with them...


Nutritional implications in wound healing. Healing of wounds is one of the life functions which is intended as a defense mechanism to maintain the integrity of the body. Understanding basic factors that guide the healing process will help the clinician to propitiate those nutritional measures known to enhance the repair function. Nutrients are normally required for growth, development, and maintenance of the integrity of the tissue. The sum total of the nutritional requirements of all tissues and organs constitutes the requirements of the individual. Recommended dietary allowances have been defined for population groups in the United States for different ages and sex. These allowances involve some 17 different nutrients, but many others (up to 50) are now considered essential, although their exact requirement may not be known. When trauma of any sort is inflicted on an individual, both local and general metabolic changes take place which may lead to nutritional inadequacy. These reactions are normally intended to set into motion the mechanisms designed to repair injured tissues. Nutritional management of the individual requires that the clinican determines what, how, when, and how much nutritional supplementation should be given to the patient. Guidelines for the amounts of proteins, vitamins, and certain minerals to be administered have been discussed. Where there are impediments that make it impossible to feed the patient orally, the clinician should resort to parenteral feeding to insure that nutrients are provided for the convalescing patient. Because the trophic environment will determine to a large extent the speed with which tissues will recover, nutritional care of the patient is important and should be interrelated to the total care of the patient.


Nutrition in growth and development of oral tissues. The development of teeth, salivary glands, and other oral tissues can be seriously compromised by a deficiency of a number of essential nutrients. Because nutritional stress during critical development periods may decrease the ultimate resistance of the oral tissues to challenge, the prevention of disease and maintenance of oral health are intimately dependent on the nutrient supply during development. Appropriate dietary counseling, particularly with reference to critical developmental times and intake of essential nutrients, can insure optimal oral tissue development.


Clinical and laboratory diagnosis of nutritional problems. The objective of this article is to familiarize the dentist with clinical signs and laboratory methods used in diagnosing nutritional deficiencies, and to indicate which laboratory methods may be useful to the clinician in cases of suspected nutritional deficiency. It should be noted that the suggested laboratory methods were selected on the basis of their applicability for the clinical situation as well as their reliability as indicators of nutritional status. Therefore these suggested methods of choice may not in every instance be the most accurate of all indicators of nutritional status for a particular nutrient. The dentist who wishes to utilize one of the laboratory methods has a number of options. He can take the appropriate sample in his office, or refer the patient directly to a clinical laboratory for the simpler analyses, or refer his patient to a physician for appropriate metabolic testing. The first option may be more appropriate for the dentist practicing in areas where a clinical laboratory is not within reasonable distance. In this instance the dentist should contact the laboratory for specific information, such as sample volume and special instructions for taking, handling, and shipping the sample. The second option is available to the dentist practicing in an urban area where clinical laboratory facilities are readily available. Finally, the dentist should work in conjunction with a physician when complex metabolic testing is required.


Triphasic nutritional analysis and dietary counseling.


Role of nutrition in the oral health of the aging patient. The food, diet and nutrition needs of the elderly patient should be considered a part of his total supportive management. It is incumbent upon the dentist to provide the patient with this nutritional information for optimal oral health, because what is good for preventing oral disease will be equally good for preventing general illness. Essentially, if the clinician keeps in mind that the calorie needs for an older patient are less but all other nutrient requirements are as high in old age as they were in youth, the geriatric patient will be well treated. Certainly there are distinctive environmental, physiological and psychological problems that must be considered, but these should not be imponderable. The best possible general advice is that daily diets should include meat, milk, vegetables and fruit, and bread. We suggest an emphasis on good quality protein foods and a generous selection of vegetables and fruits and somewhat less stress on fats, starches, and sugars to avoid an excess of calories. For the individual geriatric new denture wearer we might add that each diet prescription should be based on an analysis and evaluation of his individual food habits (the "why" of the diet) and actual food intake (the "what" of the diet). Furthermore, the physical nature of the diet should be consistent with the patient's experience and ability to swallow, chew, and bite with his dental prosthesis.


Behavioral implications in successful dietary counseling.


Why cancer patients have anorexia.

Why cancer patients have anorexia. The theory has been advanced that the anorexia of cancer is the result of anorexigenic peptides and of other intermediary metabolites produced by the cancer and the tumor-bearing host. These metabolites are the signals to peripheral receptors and to the brain centers and are responsible for the state of satiety and aversion to food. Although the only effective way to stimulate the appetite of the cancer patient is to control the cancer, efforts should be made to increase the calorie intake even in the presence of anorexia and to maintain a calorie equilibrium. However, controlled studies have not shown that forced feeding can reverse for long periods the progressive tissue wasting process or prolong the cancer patient's survival.


A comparative psychological study of the Samaritan community -- developmental aspects as revealed by the Raven, D-A-P and Bender Gestalt tests.


The emotional burden on a rural general practice.


"Obsessive psychosis": justification for a separate clinical entity.


Perception and reading ability in B.I. children.


A demographic analysis of the components of selection in a population of Arctic Skuas. From previously published demographic data of the age distributions and reproductive rates of the pale, intermediate and dark phenotypes of the Arctic Skua, revised estimates are obtained of the intrinsic rates of increase and selective coefficients of the phenotypes in each sex. Two significant components of selection are variation in age of maturity and variation in reproductive success. Sexual selection is a component of the variation in reproductive success. Dark and intermediate birds have a greater reproductive success than pales, partly as a result of their advantage in sexual selection. Pale birds have an overall advantage, however, because they are younger when they first breed. Given the estimated selective coefficients, a computer model predicts that pale birds will gradually replace the others. So far there are not enough data collected over a sufficiently long time to test the model rigorously.


Toward obliterating sex-role dichotomy: an alternative conception.


Is sex maintained to facilitate or minimise mutational advance? There are two alternative hypotheses for the selective advantages of sex: (i) The "Fisher-Muller" model:sex facilitates evolutionary adaptation to chaning environments. (ii) The "Rachet" model: sex minimises the mutational load. The relative importance of these hypotheses is discussed with reference to (a) comparative data on sexual and asexual reproduction, (b) the timing of sex in species with asexual/sexual alternation, (c) the advantages of haploid/diploid alternation, (d) the disadvantage associated with the recombinational load. It is concluded that the Ratchet model may well be the major mechanism which maintains sex.


Enzyme variability in natural populations of Daphnia magna. IV. Ecological differentiation and frequency changes of genotypes at Audley End. Genotypic frequencies were analysed for two years in a permanent population of the cladoceran crustacean, Daphnia magna, which was polymorphic for an esterase and for malate dehydrogenase. Large temporal changes in genotypic frequencies occurred at both loci. There was no evidence of a seasonal pattern in the frequency changes. In most samples, genotypes at the two enzyme loci were non-randomly associated; these associations showed temporal changes. On some occasions marked spatial heterogeneity in genotypic frequencies existed within the population. Genotypic differences in parthenogenetic and sexual egg production were observed. In a primarily parthenogenetically reproducing population, non-random associations between genotypes of structural and regulatory loci will be the rule. The allozyme variants themselves may or may not be under selection. The relevance of these observations to ecological studies on Daphnia is considered.


The colour polymorphism in Enoplognatha ovatum (Clerck) (araneae: theridiidae)--temporal stability and spatial variability. Colonies of the visually polymorphic spider Enoplognatha ovatum have been studied in Nidderdale, Yorkshire, for up to six years. Morph frequencies are found to be stable within colonies between years but to vary between colonies only short distances apart. The stability of the polymorphism argues in favour of powerful selection acting on this character. The nature of the selective factors are unknown but they are not those which influence time of maturity of the spiders or the mature female population size, both of which have varied between years. A genetic basis proposed for the polymorphism is considered and rejected as being incompatible with stable morph frequencies.


Annual cycles in the incidence of b chromosomes in the grasshopper Myrmeleotettix maculatus (acrididae: orthoptera). The frequency of B chromosomes has been determined for several stages in the grasshopper life-cycle, over three generations, in three natural populations spanning a steep cline in East Anglia. This has revealed a rise in B frequency in prediapause eggs, a fall during the winter in the embryos and a rise in the early nymphs in certain situations. The causes of these effects are discussed and also their importance, along with meiotic drive, in maintaining an environmentally correlated cline of stable adult B frequencies.


Protein polymorphism in the egg albumen of red grouse (L. Lagopus scoticus). Egg albumen of red grouse was separated by electrophoresis and then stained with naphthalene black. The number of dark-blue bands seen in one zone varied from three to six. The four fastest bands were probably controlled by three codominant alleles at an autosomal locus. The pattern of inheritance of the second fastest band was not clear because the sample was small.


Results of surgical treatment in 65 dogs with osteosarcoma. Clinical and necropsy findings were obtained from case records for 65 dogs treated surgically for osteosarcoma at the University of Pennsylvania Small Animal Hospital between July, 1952, and July, 1973, inclusive.  The median age of the dogs was 6 years. There were 39 males and 26 females. All but 5 of the 65 dogs weighed more than 16 kg. Sixty-two of the osteosarcomas arose from the limbs and 3 from the ribs. The median duration of presurgical clinical signs was 6 weeks, with a range of 1 day to 24 weeks. There were no statistical correlations between the postsurigical survival times and the age, sex, or breed of the dog, the site of the osteosarcoma, or the duration of the presurgical clinical signs. Of the 40 dogs with known lung metastases, 11 also had hypertrophic pulmonary osteoarthropathy. The median survival time was 18 weeks, with a range of 3 to greater than 578 weeks. Twenty-six percent were alive 6 months potoperatively, 13.8% were still alive at 9 months, and 10.7% survived more than 1 year. The total median duration of the disease was 24 weeks (range, 8 greater than 578 weeks).


Pulmonary resection for metastatic osteogenic sarcoma. A retrospective analysis of thirty patients who underwent pulmonary resection for metastatic osteosarcoma over a twenty-eight-year period revealed that most were young males with previous amputations for primary lesions of the lower extremity. In each, one to four thoracotomies had been performed, for a total of fifty-two chest operations with resection of 124 pulmonary metastatic lesions. At last follow-up, eleven of the thirty patients were alive and free of disease. At five years 28 per cent had survived: three of them were alive at more than nine years, and one was alive at nearly twenty years. Adverse survival factors identified were short tumor-free interval and multicentricity of pulmonary metastases. Long-term survival was associated with a tumor-free interval of more than two years and one to four thoracotomies at which few foci were present. As a group, patients selected for lung resection had significantly longer suvival than did patients whose pulmonary metastasis was untreated.


Parosteal (nodular) fasciitis of the hand. A case report.


Minor self-inflicted injuries to the gingivae: gingivitis artefacta minor. Three young patients with gingival injuries that were self-inflicted by a fingernail habit are presented. In each case the habit, which was provoked by a pre-existing locus of irritation, was stoped as soon as the locus (or trigger) was identified and treated. A distinction is drawn between these types of cases, (gingivitis artefacta minor), and those of gingivitis artefacta major where the habit is more deeply entrenched and the implications more serious.


The influence of trauma from occlusion on reduced but healthy periodontal tissues in dogs. The experiments were performed in five dogs fed a soft diet which allowed dental plaque accumulation. Experimental periodontal breakdown was introduced on Day 0. After 180 days trauma from occlusion was produced in 4P and P4 i.e. the mandibular fourth premolars, in the manner described by Svanberg & Lindhe (1973). On day 280 the periodontal pockets around 4P and P4 were eliminated. A notch was prepared in the root at the level of the bottom of the surgically eradicated pocket. In addition, the occlusal trauma in the 4P region was deleted. From Day 280 to Day 370 the teeth of the animals were brushed twice a day. The animals were then sacrificed, radiographs of the premolar regions were taken, and tissue sections comprising 3P4P1M (and P3P4M1) were produced and subjected to microscopic analysis. The results indicate that jiggling type occlusal trauma and tooth hypermobility are not factors which detrimentally affect healing following periodontal surgery.


Traumatization of teeth and gingiva related to habitual tooth cleaning procedures. Traumatization of oral hard and soft tissues connected with mechanical oral hygiene procedures has been reported in the dental literature. Definitions, classifications and localization of such lesions are listed, and in vivo and in vitro experiments within the field are reviewed. Existing literature suggests that hard tissue lesions are mainly due to abrasives in dentifrices, whereas gingival lesions may be caused by toothbrushing per se. Factual knowledge about the prevalence, severity and clinical significance, as well as relevant and reliable experiments, is scarce and further research on these topics seems to be indicated.


Epithelial cells associated with the development of dental plaque. Transmission and scanning electron microscopy were used to study desquamated epithelial cells in the developing and mature dental plaque. Epithelial cells were found attached to enamel as early as one hour after clean enamel cylinders were placed in the mouth, and they were seen in great numbers after two days. These cells generally had bacteria attached to their surfaces. Epithelial cells adsorbed to the surface of the enamel appeared to alter their morphology, and mineralization of these structures was also observed. Desquamated epithelial cells could play a role in the initial bacterial colonization of tooth enamel since it is known that bacteria selectively adsorb onto their surfaces. Hydroxyapatite may affect the morphologic alteration of epithelial cells adsorbed to tooth enamel. From the observation of developing and mature plaque samples, it seems that certain suprasurface pelliclelike structures might be epithelial cells.


Deciduous tooth chronology in the mandible of the domestic pig. Fetuses of known age, collected from 20 days' gestation to term, were used to characterize the chronology of deciduous tooth development within the right mandible of swine. Tooth development was first observed at 32 days' embryonic development, with the differentiation of the deciduous third molar. Bud stages for the remaining deciduous teeth differentiated within the period of 32 to 38 days of embryonic development. Although the initial histological appearance of these teeth covered a short period of time, it was apparent that each tooth continued to develop at its own rate. The deciduous second incisor and first molar reached a stage of enamel formation by the 80th and 86th day of fetal development. This is a much later stage than previously recorded for beginning enamel formation. The stages of tooth development and enamel formation for each tooth are summarized. A previous report on the distribution of the dental lamina and deciduous tooth development in the mandible of the domestic pig combined with the information presented in this report on tooth chronology provide much of the information required for future studies using the domestic pig in dental research. A fetus observed at the 74th day of development demonstrated a tooth bud for the deciduous first premolar. The development of this tooth was followed closely throughout the remainder of fetal development with the cap stage representing its most definitive form at 110 days' development. The suggested deciduous origin for this tooth could result in a reevaluation of the nomenclature for the dental formula of swine.


Patterns of crowding of permanent mandibular incisors before eruption. Observation of the radiographs taken of 37 mandibles from children ten months to seven years of age showed that the unerupted, permanent mandibular central incisors were rotated in 45 of 74 jaw halves (60.8%). The type of rotation in which the mesial aspect is directed lingually was most commonly seen (44.6%). The next most common observation was of jaw halves without any rotation of the central incisor (39.2%). The type of rotation in which the central incisor's distal aspect is turned lingually was the least common observation (16.2%). The rotation seemed symmetric in 25 jaws (67.6%).


Isoproterenol-induced changes in cell cycle kinetics of parotid gland acinar cells in 8-day-old rats. Isoproterenol (IPR) was shown to alter some parameters of the parotid gland acinar cell cycle in 8-year-old rats. Although the deoxyribonucleic acid (DNA) synthetic period (S) was decreased and the pre-DNA synthetic (G1) period was lengthened, there were no differences in the total cycle time. There was an increase in the 3H-thymidine labeling index from 35.7 to 50.8% combined with a decrease in the value of the mitotic index from 2.64 to 2.16% after IPR was administered. The results suggest that noncycling (G0) cells may be involved in the normal differentiation of the parotid gland and that IPR alters the transition of acinar cells between the cycling and noncycling state.


Seeded growth of calcium phosphates: effect of different calcium phosphate seed material. Seed crystals of OCP, TCP, and HAP are effective nucleators of DCPD in stable supersaturated solutions under conditions in which DCPD is the predominant phase. The crystals will grow with the second-order kinetics (TCa = 5.0 X 10(-3)M, Tp = 1.0 X 10(-2)M [pH, 5.60]) characteristic of DCPD. TCP is the least efficient, DCPD growth taking place only after an initial induction period. In all instances, the rate of DCPD crystallization is proportional to the square of the supersaturation with respect to DCPD, and the reaction is surface controlled. Scanning electron micrographs show an amorphous or microcrystalline precursor to the development of the characteristic platelets of DCPD. In stable supersaturated calcium phosphate solutions at a physiologic pH (TCa = 1.4 X 10(-3)M, Tp = 8.0 X 10(-4)M [pH, 7.40]), only OCP and HAP seed crystals are effective nucleators. The efficiency of OCP as a nucleator adds further evidence for its participation as a precursor in the precipitation of HAP under these conditions.


Heart rate and physical activity of children during dental treatment. Children with grossly observable physical activity during intraoral injection have significant heart rate accelerations. Children in whom movement is absent or confined to the muscles of facial expression experience heart rate deceleration. Both groups of children show anxiety, hence heart rate cannot be used to index anxiety. Moreover, anxiety cannot be described along a single dimension of arousal.


Studies on hypoxia: XII. Detrimental effects of synthetic polyribonucleotides on epiphyseal plates of rats exposed to hypoxia. The effect of different doses of polyadenylic and polyuridylic acids (poly A:U) was studied in control rats and in rats exposed to hypoxia. In the control rats, administration of different doses of poly A:U did not change the thickness of the epiphyseal plate or increase the incorporation of 3H-phenylalanine as judged using radioautography. Rats exposed to hypoxia showed a significant dose-related reduction in the thickness of the epiphyseal plate and 3H-phenylalanine incorporation.


Concentration of thiocyanate and ionizable iodine in saliva of smokers and nonsmokers. The concentration of thiocyanate and ionizable iodine was determined with saliva samples from two test groups, smokers (N = 27) and nonsmokers (N = 92). The contents of both ions were also compared as to sex and the amount of cigarettes smoked. The concentrations of the ions studied were dependent on each other-the less iodine, the more thiocyanate in the samples. Females had significantly more iodine and less thiocyanate concentrations than males. Smoking had an increasing effect on the concentration of thiocyanate and a decreasing effect on the content of ionizable iodine in the saliva of the volunteers. The lower amount of iodine ions in the saliva of smokers may restrict the nonthyroidal metabolism of the thyroid hormone in humans.


Thermocycling of pit and fissure sealants. Both sealants experience early thermocyclic failure, which is manifested as marginal breakdown or separation. From the 20 to 70% failure range (Fig 5) Nuva Seal was three times as resistant to thermocyclic stressing as was Epoxylite 9075. That is, the Nuva Seal-sealed teeth had to be cycled three times more than the Epoxylite 9075-sealed teeth to effect the same failure rates. Both Epoxylite and Nuva Seal reach the same failure rate at 7,000 cycles (75% failure rate). Extremely careful visual examination is required to detect marginal separation failures of pit and fissure sealants.


Follow-up studies of cortisone teratogenicity in CD1 strain mice. The present study was done to evaluate the incidence of cortisone-induced cleft palate in the offspring of CD1 strain mice whose mothers had been injected with cortisone during their own pregnancy. The incidence of induced cleft palate was as high as the incidence of cortisone-induced cleft palate reported previously for the CD1 strain, indicating that the predisposition of CD1 mice to the teratogenic effects of cortisone is not altered significantly when the F2 generation is treated in utero with the same teratogenic agent.


Surface and lamina propria lipids of bovine gingiva. In terms of both quality and quantity, the surface lipids are noticeably different from the lamina propria lipids. The surface lipids are mainly structural in nature (cholesterol, cholesterol esters, and phospholipids), whereas the lamina propria lipids are mostly of storage types (triglycerides). The surface lipids are also more unsaturated, whereas the lamina propria lipids are more saturated. Even though gingiva is histologically similar to skin, bovine gingival surface lipids are different from the bovine skin surface lipids. Further studies are needed to investigate the importance of surface lipids in regulating the various physiologic functions of gingiva.


Production and use of the thermocouple for investigation of the effect of bioelectric potentials intraorally.


A laboratory method for assessment of dentrifrice abrasivity. This is the report of the Laboratory Abrasion Committee of the American Dental Association (ADA) Dentifrice Program relative to specific methods to measure the in vitro abrasivity of dentifrices. This method was used in two collaborative studies with six participating laboratories. These studies were designed by the ADA Dentifrice Program Statistical Committee and their interpretations form a basis for recommendations on the use of the method. This laboratory method is recommended for use in dentifrice development and may provide some basis for expectations in normal dentifrice use.


Effect of salivary pellicle on enamel subsurface demineralization in vitro. Diffusion fluxes of two anionic species through hydroxyapatite membranes were found to be reduced up to 50% by the presence of adsorbed salivary pellicles developed on the membrane surfaces. By contrast, water fluxes were only marginally affected, indicating that salivary pellicles display ionic permselectivity. This property is used to explain a remarkable protection of the enamel observed when salivary pellicles were developed on extracted teeth before exposure to acid lactate buffers.


Prevalence of cariesfree naval recruits from cities with fluoridated and nonfluoridated water supplies.


Propriety of using percentages in reporting anticariogenic studies.


Susceptibility of some plaque microorganisms to chemotherapeutic agents. Investigators have used chemotherapeutic agents topically for plaque control without knowing the drug concentration necessary to inhibit the growth of odontopathic microorganisms. S mutans, S sanguis, A viscosus and A naeslundii are important components of the plaque flora. The minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) of niddamycin, vancomycin, bacitracin, and kanamycin were determined for each organism in liquid culture. These antibiotics were selected because of their low absorption from the gastrointestinal tract. Niddamycin, vancomycin, and bacitracin had the lowest MIC, from 0.2 to 10 units/ml. Kanamycin was inhibitory only at much higher concentrations (130 to 500 units/ml). The corresponding MBC was generally higher than the MIC. A viscosus was the most resistant organism tested. These data are important in designing controlled release devices for delivering a suitable antibiotic on a continuous basis intraorally.


Competency of health professionals: an approach for continuing education.


Improved fluoride incorporation and new in vitro fluorine elemental determination. The CPA technique was shown to be very useful in nondestructively determining the concentration profiles of fluoride in extracted teeth. By means of this technique, it was found that in vitro treatment with K2ZrF6 incorporated substantially more fluoride into the enamel than NaF or APF by factors of 5 and more than 50, respectively. The concentration profile was determined from the tooth surface to a depth of 2.1 mum, with a resolution of 0.07 mum at the surface and 0.15 mum at a 2.1-mum depth. Proton-stimulated X-ray fluorescence was used to detect the elements heavier than Mg, for example, P, Cl, K, Ca, Ti, Fe, Ni, Ca, Zn, Zr, and Pb.


Effect of premature loss of deciduous canines and molars on malocclusion of the permanent dentition. The effect of the premature loss of deciduous canines and first and second molars on malocclusion of the permanent dentition was studied in 255 schoolchildren 11 years of age or older at the most recent examination of the permanent dentition. Malocclusion was evaluated by scoring malalignment (major and minor) and measurement of crowding in the anterior teeth. Children who had a premature loss of one or more canines or molars had a higher frequency of receiving orthodontic treatment of one type or another for the permanent dentition. The likelihood of need of treatment increased with the number of prematurely lost teeth. Children who had lost one or more deciduous teeth through age 9 had a greater than threefold increase in the frequency of orthodontic treatment relative to the control. Of those who did not receive orthodontic treatment, there was no detectable relationship of the premature loss of canines with the malalignment of permanent teeth. However, there was a significant effect of the premature extraction of molars on malalignment especially major malalignment of permanent teeth. No differences were noted in their effects between the first and second deciduous molars. Crowding of the anterior teeth was directly affected by the premature loss of deciduous canines.


Letter: Solution to denturism.

Letter: Solution to denturism.


Letter: Objects to sugar pamphlet.

Letter: Objects to sugar pamphlet.


Sintering of dental porcelain enamels.

Sintering of dental porcelain enamels. This study showed the effects of sintering temperature and atmospheric pressure on the density, tensile strength, and microstructure of porcelain enamels. Although the densities of the opaque porcelains were higher, they were weaker than the gingival porcelains. Gingival porcelains also glazed and sintered at lower temperatures than the opaque porcelains.


Letter: MPD syndrome treatment.

Letter: MPD syndrome treatment.


Selenium content of tooth enamel obtained from two South African ethnic groups.


Editorial: The possibility of overreacting.


Periodontal therapy: principles of pocket elimination.


Identification of the phosphoprotein in the insoluble fraction of bovine dental pulp.


Relations between occlusal interference and jaw muscle activities in response to changes in head position. The jaw muscles responded to changes in the head position. Electromyographic responses to head positions were classified as either of two types--balanced and unbalanced. The balanced type of electromyographic responses of participants with normal occlusion changed to the unbalanced type after being set with an overlay to make a premature contact artificially, and returned to the balanced type after removal of the overlay. The unbalanced type of electromyographic response of participants with occlusal interference turned to the balanced type after occlusal adjustment.


Intraoral ulcers of questionable origin.


Salivary magnesium levels in pregnant women.


Mercury: its relation to the dentist's health and dental practice characteristics. Mercury and 12 clinical chemical determinations were done on the blood samples of 1,555 dentists. The dental practice characteristics of each participant were determined by questionnaires. Seventy-seven percent of all the dentists displayed blood mercury values in the range 0 to 10 ng/ml. The mean for all dentists was 8.2 ng Hg/ml blood. The means for general dentists and specialists were 8.8 and 6.3 ng Hg/ml blood, respectively. Overall, no significant correlations were found in a comparison of blood mercury levels with clinical chemical data and with dental practice characteristics. However, general practitioners with higher blood mercury values tended to show dental practice characteristics that were conducive to producing the higher values found. The data indicate that dentists in the United States, as a group, practice good mercury hygiene.


The silent hazard: an unusual case of mercury contamination of a dental suite. Heavy exposure to mercury in a vandalized dental office suite where 20 lb of mercury was maliciously spilled on floors (some covered with rugs) and splattered on walls during a nighttime break-in is described. Monitoring of ambient mercury vapor levels after a clean-up suggested that mercury contamination was under control, but urinary mercury levels that continued to increase contradicted these findings. A further investigation showed that some sections of rugs were heavily contaminated. Unaware of this contamination, dental assistants, by regularly vacuuming the rugs, created a hazardous environment. The steps taken to resolve this problem are discussed, and the importance of the urine analysis for the monitoring of mercury exposure is emphasized.


Acinar cell cycle of developing rat parotid gland: comparison of males and females.


Comments on stiffness measurements for orthodontic wires.


Mercury toxicity in the dental office: a neglected problem. Amalgam has proved to be among the most versatile and durable of all restorative materials used in the treatment of dental disease. If it is handled and controlled properly, problems with its use should not develop. Safe mercury vapor levels can be maintained if good mercury hygiene practices are exercised. Materials and devices are available for aiding the dentist in adequate mercury control procedures. With the cooperation of each member of the dental team, compliance with all the criteria imposed by governmental regulatory agencies can be accomplished. These criteria have been established for the health protection of the dentist and his auxiliary personnel. Each practioner and office staff member should realize the potential hazards associated with mercury, and effect good mercury hygienic measures and control procedures.


The mercury enigma in dentistry.

The mercury enigma in dentistry.


Development of mercury hypersensitivity among dental students. With regard to the principal purpose of this investigation, it is apparent that exposure to mercury during the preparation of silver amalgam definitely presents an additional occupational hazard as an allergen in the dentist. The fact that the dental students who were the volunteers in this study received only a small fraction of the exposure to mercury that the practicing dentist receives does emphasize the potential of this allergen in actual dental practice. Recent publicity concerning the toxioogenicity of mercury has led to  a call for improved mercury hygiene in the dental operatory. The allergenic potential of mercury, as reported in this paper, accentuates the need for such an improvement.


Odontometric discriminant function analysis of american whites and blacks.


Findings in 1,724 buccal smears from dentists. No changes were found in the epithelial cells from the buccal mucosa of dentists with the use of the light microscope, electron microscope, or electron probe that could be correlated with mercury exposure under the conditions of this test. In a normal population it can be expected that at least 0.01% of the population will show class 2 atypical smears. Patients with class 2 smears have fewer highly matured epithelial cells (tendency toward keratinization) of the buccal mucosa and hence a thinner epithelium.


Elastic constants of the composite system hydroxyapatite-dicalcium phosphate dihydrate.


Usefulness of chemical analysis of head hair for exposure to mercury.


Hand steadiness and mercury blood levels among practicing dentists: preliminary findings.


Failure to demonstrate an association between enamel fluoride concentration and dental caries in the deciduous dentition.


Hair analysis: what does it tell us?

Hair analysis: what does it tell us?


Potassium requirement of oral streptococci.


Recommendations in mercury hygiene. Council on Dental Materials and Devices.


Accurate replication of enamel for examination by scanning electron microscopy.


Erythrocyte catechol-O-methyltransferase activity in facial pain patients.


Continuing education in US dental schools. This information provides a rudimentary profile of continuing dental education in US dental schools. Some schools have wide-ranging, time-tested programs, whereas others have initiated programs only recently, yet over half of the schools mentioned that there still was an unfilled need for continuing dental education in their locality. To meet the need for quality programs in the future, the great majority of the schools (49) indicated that, although the nature and level of evaluation was open to discussion, a program of accreditation or evaluation is definitely necessary and should be implemented in the future.


The Minnesota experience: implementing mandatory continuing education.


Coordinating continuing education programs through consortia.


Continuing education courses. Course listing for July through December 1976. Council on Dental Education.


Focal epithelial hyperplasia (Heck's disease): report of case.


Ectopic movement of an unerupted mandibular canine. A case history has been given with the radiographic evidence of the migration, during development of an unerupted mandibular canine from its normal position. In a 30-month period the canine assumed a horizontal position and moved to a site that was apical to the mandibular incisors, still in the confines of the cortical plates. Because erratic movements of unerupted teeth cannot be anticipated, it would seem wise to defer the elective removal of permanent teeth during the mixed dentition stage until the clinician is assured of a normal eruption pattern of the succedaneous teeth.


Editorial: There is still a price for freedom.


Dentists' fees and inflation. Joint report of the Bureau of Economic Research and Statistics and the Bureau of Public Information. Dentists' fees, as measured by the Consumer Price Index, increased at about the same rate in the past eight years as average prices in the economy. Between 1967 and 1975, dentists' fees increased 61.9% compared with a 61.2% increase in average prices of all goods and services measured in the index. In the years since 1967, the cost of conducting a dental practice has steadily increased. Dentists' expenses for materials and services increased approximately twice as fast as the increase in dental fees. On the basis of ADA survey data, 1-3 dentists' professional expenses increased 133% and dentists' salary expenses for auxiliaries increased 148% between 1967 and 1975, whereas dentists' fees increased only 62%.


Pit and fissure sealants. Council on Dental Materials and Devices.


1976 National Children's Dental Health Week. Bureau of Dental Health Education.


Constitution and bylaws.

Constitution and bylaws.


Letter: Denturism and the dentist.

Letter: Denturism and the dentist.


Letter: Disagrees with article.

Letter: Disagrees with article.


Letter: 15-year-old views.

Letter: 15-year-old views.


Letter: An ethical point.

Letter: An ethical point.


Letter: Dental shortage myth.

Letter: Dental shortage myth.


Letter: Dentistry and healing.

Letter: Dentistry and healing.


Hypertension detection and control: importance stressed at Dental Leadership Conference on High Blood Pressure.


Iatrogenic exfoliation of teeth by the incorrect use of orthodontic elastic bands. Four cases of improper use of elastic bands for minor orthodontic treatment are described. For all the patients, the treated teeth were damaged and local surgical intervention was necessary. To prevent complications when elastic bands are being used, the dentist must provide stabilization of the elastic bands by brackets, bands, or bonded attachments, inform the patient about the possibility of losing the rubber elastic band, and mark the elastic rubber by radiopaque material. Every effort must be made to preserve the damaged anterior teeth by fixation and surgical treatment.


New instrument for finishing of embrasure margins of class II cavities.


Orthognathic surgery: combined maxillary and mandibular alveolar osteotomies with variations of surgical modalities.


Regional enteritis associated with acute myelogenous leukemia: report of case.


Isolation of mycobacteria from dairy creamery effluent sludge. Sixty three samples of dairy creamery effluent were examined for the presence of mycobacteria. Thirty two strains were isolated from 27 samples. These were classified as follows: M. fortuitum (13), M. peregrinum (6), M. gordonae(5), M. marianum (scrofulaceum) (4), unidentified (4). Ten strains, representative of the groups isolated, were tested for their effect on experimental animals. None were pathogenic for guinea pigs or mice but a number produced a minimal amount of skin sensitization in guinea pigs to avian and mammalian tuberculin.


Prevention of low temperature denaturation injury in T4Bo phage by low concentrations of traditional cryoprotective additives. The well known cryoprotective additives glucose, sucrose, glycerol, dimethyl sulphoxide, polyvinylpyrrolidone, dextran and ammonium acetate have been found to prevent inactivation of T4Bo phage frozen in sodium bromide solutions. Their protective effects in this experimental system could not be accounted for by a colligative mechanism. It is proposed that they may act by modifying the structure of the unfrozen aqueous phase rather than by direct interaction with the phage.


A clinical trial of WRL 105 strain live attenuated influenza vaccine comparing four methods of intranasal vaccination. A single intranasal dose of 10(7-0) EID50 recombinant WRL 105 strain live attenuated influenza vaccine was administered intranasally to 193 volunteers either as nose drops or by one of three spray devices which produced sprays of differing physical characteristics. In volunteers with homologous haemagglutinating inhibiting antibody titres of less than or equal to 20 before vaccination, seroconversion rates varied widely from 80% following the administration of drops to 71%, 57% and 28% with the three spray devices. In the week following vaccination 16 (22%) of 74 volunteers who were found to show a fourfold or greater antibody response to took analgesics to control symptoms in comparison with 4 (7%) of 58 volunteers who exhibited no serological response to vaccination (P less than 0-05). However, neither the occurrence of upper respiratory nor systemic symptoms were significantly different in these two groups and the degree of attenuation of the recombinant WRL 105 strain appears to be acceptable for future use.


Platelet-induced tolerance in the production of heteroantisera to human leukemia-associated antigens. A method is described for raising antisera to human leukemia cells of an individual patient in mice rendered tolerant with cyclophosphamide to platelets obtained from the same patient. The resulting antisera are able to distinguish serologically between leukemic blast cells and remission cells of patients with acute leukemia and may be recognizing leukemia-associated antigens. The antisera are similar in activity to antisera raised following  tolerance-induction with remission leukocytes, but larger volumes of anti-leukemia antiserum can be raised using the more easily obtainable platelets. This technique provides further evidence that human platelets share many of the antigens present on human leukocytes.


Acute infections in interferon-treated patients with osteosarcoma: preliminary report of a comparative study. The incidence of acute infections in eight patients with osteosarcoma who are receiving interferon regularly is currently being compared with the incidence among their family members. Observations thus far indicate that the patients treated with interferon are less frequently and less severely ill than their untreated family contacts. The study is continuing, and the patients will also be observed after discontinuation of interferon treatment. Sera are regularly frozen for future viral serologic tests. The preliminary clinical data indicate that therapy with exogenous interferon may afford protection against common acute infections.


A solid-phase radioimmunoassay for the measurement of lactoferrin in human plasma: variations with age, sex, and disease. A solid-phase radioimmunoassay is described for measuring lactoferrin levels in normal human plasma. The sensitivity of the assay was 6 ng. per milliliter with an intraassay coefficient of variation of 4 per cent and an interassay value of 9 per cent. Healthy adult males had a mean plasma level of 1.62 mug per milliliter which was significantly higher than adult females, 1.07 mug per milliliter. Postmenopausal females had levels similar to men, 1.74 mug per milliliter, while younger women had a significantly lower mean value, 0.75 mug per milliliter. Two menstruating women and 2 pregnant women had moderately elevated levels. Consistently elevated levels were found in patients with untreated or relapsing chronic myeloid leukemia--all over 12.0 mug per milliliter, while patients on marrow suppressant therapy tended to have subnormal levels. The collection of serum specimens as opposed to plasma, resulted in inconsistently elevated levels: EDTA was the anticoagulant of choice, as heparin interfered in the radioimmunoassay system.


Sex dependence of the metabolic activation in vitro of the mycohepatocarcinogen aflatoxin B1. Using liver microsomes, metabolic activation of aflatoxin B1, determined by the formation of DNA- and protein-binding metabolites, was studied in an effort to elucidate the possible basis underlying sex differences in sensitivity of the male and female rat to aflatoxin B1. Male rats, which are more sensitive to AFB1, produced significantly higher amounts of these metabolites than female rats; castration of the male reduced the level to that of the female, whereas ovariectomy was without any effect.


Acute pulmonary edema and hyperosmolality: a clinical study. Patients in the coronary care unit with acute pulmonary edema, heart failure, and other organic heart disease were studied. Blood and urine samples were taken on admission prior to any treatment and later at prescribed intervals. All the patients with APE were found to have elevated plasma osmolalities and hyperglycemia on admission which decreased with treatment. This was in contrast to the other two groups excluding those factors such as ethyl alcohol and diabetes which can raise plasma osmolality or blood glucose. A discussion of this mild hyperosmolal state in APE follows including possible causes as well as cellular effects of hyperosmolality on humans.


Alkali-labile regions and strand breaks in DNA from cells treated with daunorubicin. Lymphocyte cells (P-288) in culture were exposed to daunorubicin (Dm) and analyzed for DNA damage by means of alkaline sucrose and neutral (formamide) sucrose gradients. In neutral gradients, the sedimentation rate of DNA from cells treated with 25 X 10(-8) M Dm for brief periods up to 60 min is the same as that of the DNA from control cells (i.e., drug-treated for "zero" time). In contrast, extensive DNA damage is already apparent in alkaline gradients after treatment of the cells for only 30 min. After treatment for 2 hr, single-strand damage is evident in both types of gradient. These results demonstrate the introduction of alkali-labile regions in the DNA of cells treated for 45 or 60 min with Dm. The DNA damage is concurrent with inhibition of thymidine incorporation, and both may occur at threshold levels without affecting subsequent growth of the P-288 cells after short exposures to low concentrations of the drug.


Response of murine DNA polymerases C and N to partial hepatectomy. Two DNA polymerases, designated C and N have been characterized in extracts of murine spleen and liver (Evans et al. 1974). The effect of partial hepatectomy on the activity levels of these two enzymes was studied by gel filtration of crude extracts on Sephadex G-200. Both enzymes were observed to respond to the proliferation stimulus. Polymerase N activity reached maximum levels between 30 and 48 hours after hepatectomy. Polymerase C response was delayed until 30 hours and reached maximum values at 48-72 hours.


Uptake of adriamycin and daunomycin in L1210 and human leukemia cells: a comparative study. The uptake of Adriamycin (AM) and Daunomycin (DM) in L1210 leukemia cells was found to be dependent on drug dose, time, and temperature. DM was taken up to a greater extent in human leukemia cells in vitro and in L1210 leukemic cells both in vitro and in vivo. Intracellular retention of DM was less than that of AM. Drug transport appears to be a factor in developing resistance to DM in L1210 cells, but the data for AM were inconclusive in this regard. The importance of cellular uptake and retension operative in determining differences in the overall therapeutic efficacy in vivo remains to be elucidated.


The effect of pretreatment with allogeneic fetal cells on hamster melanoma. Pretreatment with 7-day embryonic tissue mixed with incomplete adjuvant retarded the growth of melanoma transplants in the Syrian hamster. This effect was not apparent in animals treated with fetal tissue suspension, only or with fetal tissue and complete adjuvant. The inhibitory effect on tumor growth noted in animals challenged with tumor cell doses of 5 x 10(5) or 2.5 x 10(6) cells was abolished when the tumor dose was increased to 5 x 10(6). These results suggest that hamster melanoma and fetal tissue may share a common antigen.


Dose distribution of therapeutic electron beams and automation of treatment planning. Until the present time, there has been no mathematical evaluation of the therapeutic electron beams in tissue material. Electron treatments are becoming universally applied from betatrons and linear accelerators. There is, therefore, a need for dosimetric programming of hospital computers for electron treatment planning such as those used in photon beam dosimetry. In this study, we have developed a theoretical model for dose calculation in a clinical therapeutic electron beam (Osman, 1972 and 1973). On the basis of this proposed theory, one can predict dose profiles at any source to skin distance "SSD"' and at any depth in tissue "X". Our theoretical model is based on considering the clinical broad electron beam used in radiation therapy as being made up of an infinite number of identical and initially parallel pencil beam components, each of minute width, to which the existing theories (Lewis, 1950; Attix et al., 1968) on electron multiple scattering, in the concerned medium apply. Dose profiles in tissue, as obtained from this model, could provide useful information as input data for routine programs of treatment planning with high energy electron beams, using mini-computers. Further, it is also possible to account for any body inhomogeneity such as subcutaneous fat, lung, air volumes, body cavities, fluids and bone from the basic parameters of these media.


Effects of radiation and porphyrin on mitosis and chromosomes in human hematopoietic cell lines. The effect on mitosis of a human hematopoietic cell line RPMI-1788 treated with a metal chelate (Zn++) of meso-tetra (p-carboxyphenyl) porphine (Zn-TCPP) alone at various concentrations or in combination with gamma-irradiation at various doses were studied. The results showed that both Zn-TCPP and radiation were effective in interfering with normal mitosis and that the effect of radiation was relatively more effective. Data also suggest interacting effects between Zn-TCPP and gamma-irradiation. At low doses of radiation, Zn-TCPP potentiated the effect of radiation. The reverse seemed to be true at a high dose of radiation. The effects of two porphyrins (Zn-TCPP and hematoporphyrin) and radiation on chromosomes were also studied. Chromosomal aberrations characteristic of radiation were observed. The porphyrins were found not to be effective chromosome-breaking agents under the experimental conditions tested.


Tensile strength, relaxation and mechanical recovery in rat skin as influenced by maturation and age. Several mechanical parameters were measured in skin strips of rats at various ages (1, 2, 4, 12 or 24 months). Ultimate load and tensile strength increased during the maturation process and reached a maximum at 12 months. In old animals (24 months) a decrease of these parameters was noted. After various preloads relaxation was measured. Relaxation could be described mathematically as a function of logarithm of time. The relaxation coefficient decreased during the maturation as well as during the ageing period. After unloading up to 1/5 initial load mechanical recovery was measured. Mechanical recovery was increased depending on age during the whole life span. It is concluded that various components of viscoelatic behaviour are influenced differently by the maturation and the ageing process.


Correlation of response to 1-beta-D-arabinofuranosyl cytosine and metabolism of drug by tumor. Phosphorylation versus deamination of ara-C by tumor homogenates was measured in 10 patients prior to treatment with ara-C infusion. The ratio ranged from 0.24 to 1.2 in 5 malignant melanomas, 2.6 to 3.2 in 3 histiocytic lymphomas and 0.9 in a hemangiopericytoma. Treatment of the 9 patients with 2 courses of 5-day continuous ara-C infusion failed to produce objective evidence of tumor regression. The tenth patient had lymphosarcoma leukemia. The baseline ratio of ara-C phosphorylation over deamination activity of the tumor cells was 1.23. Treatment with 2 courses of continuous ara-C infusions produced a brief state of complete remission. The ratio of araC phosphorylation over deamination activity in the tumor cells after relapse was only 0.1 and retreatment with ara-C infusions failed to produce antitumor response. This study indicates that factors other than a high ratio of phosphorylation over deamination activity by the tumor seem to play an important role in the susceptibility of tumors to ara-C treatment.


A simplified method of treatment for endodontic perforations.


The incidence of bacteremias related to endodontic procedures. I. Nonsurgical endodontics.


Microorganisms isolated from pulp chambers.


Measurement of tooth temperature as a means of determing pulp vitality.


How penicillin works.

How penicillin works.


Physical properties of root canal cements.


Reaction of rat molar pulp tissue to Formocresol, formaldehyde, and cresol.


Apexification: report of case.

Apexification: report of case.


The educational preparation of the psychiatric nurse generalist.


The psychiatric nurse as a specialist in clinical practice.


The nurse administrator: survival in the executive jungle.


The code for nurses: a guide for ethical nursing practice.


Some angles to consider.

Some angles to consider.


The law, the nurse, and the rape victim.


Letter: The dentist's role in oral cancer detection.


The associate degree nursing faculty and the resolution on entry into professional practice.


A nurse views education, needs and involvement in society.


Editorial: Prophylactic antibiotics: a problem or panacea?


Oral changes in Crohn's disease.

Oral changes in Crohn's disease. Oral lesions in patients with Crohn's disease may be characteristic granulomatous lesions, secondary results of deficiency conditions, or coincidental lesions. Thorough investigations are necessary to rule out involvement of the gut in the disease process. If results fail to substantiate a diagnosis of Crohn's disease, regular follow-up examinations are necessary to detect possible later development of the disorder.


Reconstruction of the orbital floor using lyophilized dura: report of case.


Clinico-pathological conference. Case 16, part 1.


The Robin anomalad - its nonspecificity and associated syndromes. The Robin anomalad, once thought to constitute a specific syndrome, is now known to occur with several genetic syndromes, a few drug-induced syndromes, and some loosely associated anomalies. The mode of inheritance in genetic syndromes with the Robin anomalad is that of the particular syndrome with which the anomalad is associated.


Treatment of central hemangioma of the maxilla by embolization: report of case. Some methods of treatment for cavernous hemangiomas are reviewed and briefly discussed. Artificial embolization with barium-impregnated spheres was used by us because any other method of treatment seemed to be inadvisable. Techniques of the surgical procedure were followed as programmed by Lussenhop and others, 12 Longacre and others, 2 and by Hoey and others.9 A 47-month follow-up with no radiographic evidence of residual hemangioma has indicated a satisfactory result in this case.


Unilateral relapse after oblique osteotomy of the mandibular rami: report of case. A case of unilateral relapse of mandibular prognathism after oblique osteotomy of the mandibular rami is described. A brief review of the pertinent literature is presented and a method of intraosseous fixation by Vitallium plating is illustrated. The need for close postoperative observations and reinstitution of active therapy at the first sign of a relapse tendency is emphasized.


Inappropriate secretion of antidiuretic hormone after cerebral injury. A case has been presented in which a patient sustained a closed head injury with concomitant maxillofacial injuries; early signs of water intoxication and ISADH developed six days after injury. This disorder was corrected by restricting free water intake for six days until equilibration occurred. Successful reduction of the facial fractures was accomplished after stabilization of the patient's neurological condition and correction of her metabolic disorder. The ISADH and resulting hyponatremia have been documented in a variety of disease states including trauma to the central nervous system. Disruption or irritation to the hypothalamic-neurohypophyseal system has been proposed as the mechanism of dysfunction after cerebral injury. The results of the secretion of inappropriate amounts of ADH relative to renal function and homeostatis have been discussed. Clinical and laboratory diagnosis as well as the elective and emergency management of ISADH have been reviewed. The fact that the sequelae of this abnormal metabolic state may mimic or mask the neurological deterioration which may follow cerebral injury is significant. This may contribute to the difficulty in making a correct diagnosis and designing proper therapy. The problem is basically one of differentiating a correctable metabolic disorder from a lesion that can be fatal unless surgically removed.


Correction of class II malocclusion by combined sagittal ramus and subapical body osteotomy. The complete mandibular subapical osteotomy has been presented as a method for correction of Class II malocclusions. At this point, it holds some promise as a solution to the skeletal relapse seen in the surgical correction of Class II malocclusions with use of other techniques. The procedure is a straight-forward combination of the sagittal osteotomy as described by Obwegeser and the subapical osteotomy as described by Köle. Further investigation into the versatility of this procedure is recommended.


Crohn's disease of the mouth: report of case.


Epidural hematoma after minor oral trauma. A case report was presented in which a 15-year-old boy was beaten about the head with a baseball bat. Intraoral trauma and facial lacerations were repaired. Since results of the neurological examination were within normal limits, the patient was discharged. The next day, the patient became lethargic; however, the patient's mother did not bring the patient back to the hospital until the routine postoperative visit. At that time, the patient had right hemiparesis, was unable to speak, and was clearly obtunded. A carotid angiogram disclosed a left venous epidural hematoma in the parietal area. A craniotomy was performed with good results. The importance of follow-up neurologic examinations in cases of trauma to the face and head is stressed.


Squamous cell carcinoma of the submandibular gland.


False aneurysm of the facial artery.

False aneurysm of the facial artery. A false aneurysm with typical history, clinical and radiographic findings, and histological picture is reported. The absence of enlargement since onset and the absence of bruit were atypical. The latter was probably due to the thick wall of the aneurysm. False aneurysm should be part of the differential diagnosis of swelling of the jaws. Aneurysms should be treated surgically because of the potential for progressive enlargement with danger of spontaneous rupture.


Electrical stimulation of osteogenesis in pathological osseous defects. Although the literature on bioelectric phenomena is extensive, there is a paucity of research on its application in the field of dentistry. This study was designed to evaluate the effect of bioelectrical stimulation on pathological osseous defects in the periodontium of Beagle dogs. Galvanic and thermocouple generators were designed to deliver a current in the nanoampere range. The galvanic unit produced a constant direct current. The thermocouple unit was designed to deliver an oscillating dc current. The generators were implanted in the area of periodontal osseous defects in five dogs. These sites were randomly designated as experimental, active controls, and passive controls. The experimental and control units were implanted submucoperiosteally with the negative electrodes extending into osseous defects. The bone was labeled with Procion Red H-8BS at 1 week. Clinical and radiographic evaluation revealed no significant changes in the depth of the defects. The animals were sacrificed at 45 days and block sections were prepared for histological measurement. From the results of this study it was concluded: 1. Induced constant or oscillating negative dc current levels in the nanoampere range were unsucessful in causing regeneration of periodontal osseous defects either clinically or radiographically in the Beagle dog over a 45-day period of application. 2. Constant or oscillating negative dc current levels in the nanoampere range did not stimulate a significant amount of periosteal osseous apposition. 3. Oscillating negative nanoampere dc currents produced an increase in endosteal osseous apposition as compared to controls. 4. Constant negative dc current in the nanoampere range produced an increase in endosteal osseous apposition as compared to controls. 5. The histological density of the bone in the area of pathological periodontal defects was increased in all experimental sites as compared to controls. 6. Devices of comparable size and construction to both the galvanic and thermocouple electronic units used in this study are acceptable for future investigation of the effect of bioelectrical stimulation on osteogenesis in alveolar bone.


A polystyrene mandibular pressure harness for osteotomies and genioplasties.


Osteosarcoma of the thyroid gland.

Osteosarcoma of the thyroid gland. A case of osteosarcoma of the thyroid gland in a 61-yr-old woman who died from tracheal compression due to local recurrence 6 wk after operation is presented. The main features of 27 previously reported similar cases, and the problems of histogenesis are discussed.


Vital root amputation. A clinical and histological study. An investigation was designed to study the clinical and histological effects of delaying endodontic therapy for a period of 2 weeks without dressing of the exposed pulp, after vital intentional root amputation had been carried out on periodontally involved teeth. From the results obtained it was possible to conclude that: 1. Endodontics, prior to surgical root amputation, remains the treatment of choice. 2. When preoperative endodontics is not possible, the approach used in this study will provide good results without severe, adverse clinical or histological effects. 3. The altered periodontal condition and tooth form do not interfere with the performance of definitive endodontics 2 weeks after surgery. 4. Definitive preparation of the cut tooth surface at the time of root amputation facilitates maintenance of oral hygiene after removal of the periodontal dressings. 5. The high percentage of involvement of the distobuccal root of the first maxillary molar in periodontal situations requiring correction by root amputation, may be due to the anatomy of this root.


Epithelial changes incident to tooth movement: a radio-autographic analysis.


Study of gliding tooth contacts during mastication. Tooth gliding occurs frequently both in the opening (55.9%) and closing (60.5%) chewing strokes. The average glide length was 1 mm. Good occlusions could not be distinguished from poor occlusion on the basis of frequency and length of gliding contacts. The greater the component of lateral movement during the closing stroke, the greater the probability that gliding contacts would occur.


Vitamin E and periodontal disease.

Vitamin E and periodontal disease. Vitamin E levels in patients with and without periodontal disease were measured. There was no significant difference in the levels of serum vitamin E between these two groups. These data do not provide support for the treatment of inflammatory periodontal disease with vitamin E.


The effect of urea peroxide on dilantin hyperplasia. 1. A significant inverse relationship was found between the severity of the hyperplasia and the duration of SDH. 2. The severity of hyperplasia and in flammation was found to vary significantly and inversely with oral hygiene scores. 3. Tooth brushing twice a day and the application of Oxygel showed no significant clinical changes on the inflammation and hyperplasia in the test group, whereas the control group using a placebo showed a moderate reduction in gingival inflammation.


The destructive potential of electrosurgery on the periodontium. Three cases have been presented illustrating the complications which may arise from the use of electrosurgery. These cases provide clinical evidence of the potential for severe periodontal destruction, a potential that must be weighed carefully by any practitioner who uses an electrosurgical instrument. It is suggested that new scientific studies be initiated to establish basic principles that will eliminate the hazards that now exist in the routine use of electrosurgery.


Metastatic adenocarcinoma of gingiva. Report of a case. A case of metastatic neoplasm developing in the gingiva from a primary lesion, probably sited in the gastric mucosa, has been reported. In this case, the first demonstrable metastatic lesion was located in the gingiva. The dentist has a great responsibility in detecting malignancy in the oral cavity because of his opportunities during routine examinations.


Tolerance and limited cross-tolerance to the effects of N, N-dimethyltryptamine (DMT) and lysergic acid diethylamide-25 (LSD) on food-rewarded bar pressing in the rat. Lysergic acid diethylamide=25 (LSD) and N,N-dimethyltryptamine (DMT) abolish food-rewarded, fixed-ratio bar pressing by rats in a dose-related fashion. Adult male Holtzman rats trained to press a bar (respond) for milk reward on a 4-response fixed-ratio schedule were given i.p. injections of 3.2 or 10 mg/kg of DMT every 2 hours for 21 days. Every 24 hours the animals were placed in operant chambers for 30 minutes before a scheduled injection and were left in the chambers for 30 to 80 minutes after. During the first week of chronic treatment, daily bar pressing worsened progressively until the 6th day of the series, at which time rats in the 10 mg/kg group did not bar press at all. As the chronic injections continued, rates of bar pressing gradually increased until responding was not disrupted at all by an injection of DMT. Rats in the 3.2 mg/kg group showed cross-tolerance to an injection of LSD (0.1 mg/kg). Another group of rats was made partially tolerant to the disruptive effects of LSD (0.1 mg/kg i.p.) on bar pressing with a series of injections given once per day for 21 days and then three times per day for the next 4 days. Cross tolerance was not demonstrated to a challenge injection of 10 mg/kg of DMT. The LSD injections were continued for another 3 to 5 days until the animals were completely tolerant to the LSD. They then displayed cross-tolerance to 3.2 mg/kg of DMT.


Proliferation and production of hemopoietic cells two stages of disease: preleukemia and overt leukemia. The kinetics of erythropietic and granulocytopoietic cell proliferation have been investigated in the same patient at two distinct stages: firstly in preleukemia presenting as pancytopenia with ineffective erythropoiesis, and secondly 2 years later in acute myelogenous leukemia. The method investigation is based on determining the DNA synthesis rate of individual cells by means of quantitative 14C-autoradiography after short-term incubation with 14C-thymidine and fluorodeoxyuridine. Erythropoiesis was equally ineffective in the two stages, the rate of proliferation, however, slowed down towards the leukemic state. The production rate of myeloblasts was markedly reduced in preleukemia accompained by a very low labelling index. In leukemia on the other hand the production rate was increased to such a degree that the leukemic myeoblast compartment is to be considered as prevailingly self-reproductive. The proliferation rate of myeloblasts was reduced already in preleukemia, and there was a further decrease in leukemia. From the point of view of cell kinetics the manifestation of leukemia in this patient is explained best by a change in the mode of proliferation: the myeloblasts change from steady state growth to behaving like an exponentially expanding population.


S. C. Department of health and Environmental Control's Perinatal Program.


Identification of the high risk obstetrical patient: the use of an objective scoring method.


Amniotic cell 4-methylumbelliferyl-alpha-glucosidase activity for prenatal diagnosis of Pompe's disease. Using a simple fluorometric assay for alpha-glucosidase activity of cultured amniotic cells, we have monitored two pregnancies from families at risk for Pompe's disease. The fetus was judged to be affected in one, the pregnancy being terminated and unaffected in the other. The accuracy of these predictions was confirmed. These results suggest that this assay allows accurate prenatal diagnosis of Pompe's disease, three weeks after diagnostic amniocentesis. Amniotic cell 4-methylumbelliferyl-alpha-glucosidase activity for prenatal diagnosis of Pompe's disease was studied. 2 pregnancies in families at risk for Pompe's disease were monitored using a simple fluorometric assay for alpha-glucosidase  activity of cultured amniotic cells. In 1 pregnancy the fetus was judged to be affected and was confirmed after termination. In the other the fetus was judged not to be affected and was confirmed at 12 months of age.  These results suggest that this assay for alpha-glucosidase activity on amniotic cells collected at amniocentesis and cultured for about 3 weeks allows accurate prenatal diagnosis of Pompe's disease.


Wanted!! a wizard to finance adequate programs for the mentally disabled.


Human cocidiosis. Recurrent diarrhea of 26 years duration due to Isaspora belli: A case report.


In vitro granulocytic stem cell clonogenic capacity of marrow and spleen cells from mice infected with Rauscher leukemia virus. Bone marrow and spleen cells from early, midstage, and terminal Rauscher leukemia virus (RLV-A)-infected erythroleukemic mice were assessed for granulocyte stem cell (CFU-c) clongenic capacity in the semisolid agar culture assay. It was found that marrow CFU-c concentrations exceeded normal in early stages of this erythroid disease but returned to near normal values during mid- and terminal phases. Splenic CFU-c concentrations, on the other hand, were generally higher than control values for all stages of the disease. These results are discussed with reference to the pathogenesis of RLV-A disease.


Detection of hypertension.

Detection of hypertension.


Realities of "genetic engineering.''.

Realities of "genetic engineering.''.


Trauma from occlusion; cause, progression, results and treatment.


Platelet granulopathy: a new morphologic feature in preleukemia and myelomonocytic leukemia: light microscopy and ultrastructural morphology and cytochemistry. We have previously reported on the ultrastructure of platelets in preleukemia and myelomonocytic leukemia. We referred to an unusual and distinct anomaly of the platelet granules found in 15 of 16 patients. In the present communication we wish to describe and illustrate the light microscopic appearance of giant anomalous granules. Close scrutiny of the platelet morphology and a search for the aforementioned platelet granulopathy are important in the evaluation of patients with myeloproliferative diseases. In this paper we describe and illustrate in more detail the ultrastructure and ultrastructural histochemistry of the abnormal granules. In those patients with the platelet granulopathy, we have conducted in vitro platelet aggregation studies and carried out an electron microscopic evaluation of the aggregates. At least some of the giant granules remained morphologically intact in advanced stages of the aggregation phenomenon, and thus they are probably composed of elements that were not released during aggregation.


Clinically significant cardiac infiltration in acute leukemia, lymphocytic lymphoma, and plasma cell myeloma. Cardiac infiltration by hematologic neoplasms leading to clinically significant cardiovascular disease is rare. Three such cases are described in this report, and it is suggested that rare manifestations of hematologic neoplasms may become more common in the future since these diseases are more amenable to therapy than heretofore. Cardiac involvement with hematologic neoplasms is of more than academic interest since this complication is likely to respond to radiotherapy.


Cell kill kinetics with hydroxyurea.

Cell kill kinetics with hydroxyurea. The effects of various concentrations of hydroxyurea (HU) on a human lymphoid cell line in exponential growth phase have been studied using a combination of methods, including determination of the total and viable cell counts; the cells relative DNA content, measured in a flow microfluorimeter after staining with a fluorescent Feulgen technique; the mitotic index; and the percentage of cells incorporating thymidine-3H (TdR-3H) during brief and continuous exposure to the isotope both in the presence and absence of colcemid. A significant redistribution of the cells in the various phases of the cell cycle occurred during the first 24 hr of continuous treatment with 10(-3) M and 10(-2) M HU as follows: (1) division of cells in G2; (2) depletion of mid and late S phase cells due to early cell death; (3) movement of most G1 cells at a normal rate into early S phase where they accumulate; and (4) arrest of the remaining cells in G1, which represented the surviving population after treatment for 96 hr or longer. After removal of the drug, the cell fraction blocked in early S phase progressed semisynchronously through S, but many of the cells were unable to complete division. Their capacity to recover depended on the drug concentration and duration of exposure, but in general the cellular injury caused by HU was more reversible than that caused by "equivalent" concentrations of arabinosylcytosine.


Usefulness of cytosine arabinoside (NSC-63878) and prednisone (NSC-10023) in refractory childhood lymphoblastic leukemia. One hundred forty-three children with refractory lymphoblastic and undifferentiated leukemia (ALL/AUL) were treated with cytosine arabinoside (Ara-C) and prednisone (Pred). The dose and duration of Ara-C was escalated during induction depending on the response seen in the peripheral blood and/or bone marrow. For those achieving a remission, Ara-C was also used to determine its maintenance capabilities. Of the 143 children, 79 attained a clinical remission, 45 having a complete bone marrow remission and 34 having a partial remission. Maintenance of remission with twice weekly Ara-C was short and did not appear to depend on the amount of Ara-C given during induction. The major toxicity of Ara-C was myelosuppression.


Continuing education for the certified dental technician.


Editorial: Honesty is still the best policy.


Bone disease.

Bone disease.


NBS research program on non-precious alloys.


Infant mortality in Cleveland County: a recent secular increase.


Genetic transformation in Drosophila by microinjection of DNA.


Local public health departments and their directors in North Carolina and the United States.


Cardiopulmonary resuscitation (CPR) as treatment of cardiac arrest. II. Basic CPR.


Treatment in prostatic carcinoma in Western New York.


Second and subsequent periodic examinations.


Evaluation of a preevacuated endometrial suction apparatus in general obstetric-gynecologic practice.


Nitroblue tetrazolium dye reduction: diagnostic value in children with febrile illnesses.


Dry gangrene of toes: managed with foam rubber boots and ambulation.


Correlation conferences in radiology and pathology: Lytic tarsal lesion.


Nonbacterial thrombotic endocarditis in premature child: clinical significance and possible relationships to subvalvular hematoma.


Xanthogranulomatous pyelonephritis: with emphasis on angiographic findings.


Venereal amebiasis.

Venereal amebiasis.


Common bile duct obstruction due to Fasciola hepatica.


Hypogammaglobulinemia, pernicious anemia: with carcinomas of urinary bladder and lung.


Granulomatous esophagitis (Crohn's disease): associated with granulomatous enterocolitis.


Colopulmonary fistula due to perforating carcinoma of splenic flexure.


Ultrastructure of human pulmonary alveolar lesions.


Multiple simultaneous cholecystenteric fistulas.


Resection of primary mycotic aneurysm of abdominal aorta.


Chronic osteomyelitis of mandible: hyperbaric oxygenation treatment.


Hypocalcemic tetany: result of high-phosphate enema.


Fibromuscular dysplasia of internal carotid arteries: graduated internal dilatation by arterial Fogarty catheter.


Successive hydropcephalic newborns.

Successive hydropcephalic newborns.


Pulmonary artery sarcoma.

Pulmonary artery sarcoma.


Superior mesenteric artery syndrome.

Superior mesenteric artery syndrome.


High-flyer syndrome: survival after 17-story fall.


Noninvasive methods in cardiology. Brief outline presentation and critique.


Multiple myeloma with small-bone involvement.


Role of liver in erythropoietin production: clinical and experimental evidences.


Immune system: introduction.

Immune system: introduction.


Chest trauma: its nature in urban ghetto.


History of academy of family physicians in New York State.


Phrenology: bumps or bust.

Phrenology: bumps or bust.


Noise as a health hazard in the boilermaking industry. An investigation was made into noise hazard aspects of three boilermaking firms. This consisted of noise measurement of both background and impact noises, of field audiometry of the workforce and a questionnaire. Environmental findings so far show the noise to be hazardous to hearing. Audiometric testing revealed a high rate of hearing impairment. A general lack of knowledge of noise hazard was shown, and the need for a hearing conservation programme demonstrated.


Spironolactone-associated aggravation of renal functional impairment. Five patients in whom deterioration in renal function was associated with the use of the aldosterone antagonist, spironolactone, are reported. Care in the use of this drug is urged, especially in the elderly. Patients with known renal functional impairment should not receive this agent.


Management of sugar intolerance in children. Sugar intolerance is a common problem in paediatric practice. The usual type is lactose intolerance following gastroenteritis, but it may also occur in a wide variety of disorders of the small bowel. Diagnosis depends upon identification of reducing substances in the stools. An approach to dietary management of lactose intolerance is given and use of a carbohydrate-free formula in secondary monosaccharide intolerance is described. In each situation, threshold for digestion or absorbtion of carbohydrate is approached gradually from below, and overflow detected by Clinitest stool testing.


Pull-through uretero-lithotomy.

Pull-through uretero-lithotomy. A method for removing a stone from the lower end of the ureter by a pull-through uretero-lithotomy is described.


Poor man's medicine.

Poor man's medicine.


A short interview as an assessment of student health and potential. A prospective study of the effectiveness of a short interview in assessing health needs and potential performance of students. The results indicate that such an interview is of limited value in the recognition of the vulnerable student, can predict performance of the best students with reasonable accuracy, can recognise potential drop-outs, but failed to distinguish in a borderline group between those likely to succeed and those not.


Vibrio parahaemolyticus-food poisoning: case report. Symptoms of food poisoning occurred following the ingestion of raw shellfish purchased in the Auckland area. Vibrio parahaemolyticus was recoverer from the patient. The potential of this and closely related microorganisms to cause illness is reviewed.


Letter: Deaths in infancy.

Letter: Deaths in infancy.