Formation of a keratinizing epithelium in culture by a cloned cell line derived from a teratoma. From a transplantable mouse teratoma it has been possible to derive an established keratinizing cell line (XB) which grows well in cultures containing lethally irradiated 3T3 fibroblasts at the correct density. Single cells of the keratinizing line grow into colonies each consisting of a stratified squamous epithelium. The keratinizing nature of the colonies has been demonstrated by specific staining with Rhodanile blue, and by light and electron microscopy of sections through the colonies. A function of fibroblasts appears to be a strict requirement for keratinization and an important though less strict requirement for cell growth. The fibroblast function can be carried out by medium harvested from 3T3 cultures. It is possible to detect keratinizing colonies in primary cultures of disaggregated teratoma cells combined with 3T3 cells. Such colonies appeared in cultures of a transplantable teratoma with an overall frequency of 6 X 10(-6) of the cells plated. Nonkeratinizing colonies of cells with otherwise very similar appearance were about 10 fold more abundant. Since both the keratinizing and the related nonkeratinizing colonies can be identified in the living state, it is possible to isolate them from the primary cultures.


Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Human diploid epidermis epidermal cells have been successfully grown in serial culture. To initiate colony formation, they require the presence of fibroblasts, but proliferation of fibroblasts must be controlled so that the epidermal cell population is not overgrown. Both conditions can be achieved by the use of lethally irradiated 3T3 cells at the correct density. When trypsinized human skin cells are plated together with the 3T3 cells, the growth of the human fibroblasts is largely suppressed, but epidermal cells grow from single cells into colonies. Each colony consists of keratinocytes ultimately forming a stratified squamous epithelium in which the dividing cells are confined to the lowest layer(s). Hydrocortisone is added to the medium, since in secondary and subsequent subcultures it makes the colony morphology more oderly and distinctive, and maintains proliferation at a slightly greater rate. Under these culture conditions, it is possible to isolate keratinocyte clones free of viable fibroblasts. Like human diploid fibroblasts, human diploid keratinocytes appear to have a finite culture lifetime. For 7 strains studied, the culture lifetime ranged from 20-50 cell generations. The plating efficiency of the epidermal cells taken directly from skin was usually 0.1-1.0%. On subsequent transfer of the cultures initiated from newborns, the plating efficiency rose to 10% or higher, but was most often in the range of 1-5% and dropped sharply toward the end of their culture life. The plating efficiency and culture lifetime were lower for keratinocytes of older persons.


Sequence organization of the human genome. The organization of three sequence classes--single copy, repetitive, and inverted repeated sequences--within the human genome has been studied by renaturation techniques, hydroxylapatite binding methods, and DNA hyperchromism. Repetitive sequence classes are distributed throughout 80% or more of the genome. Slightly more than half of the genome consists of short single copy sequences, with a length of about 2 kb interpersed with repetitive sequences. The average length of the repetitive sequences is also small and approximates the length of these sequences found in other organisms. The sequence organization of the human genome therefore resembles the sequence organization found in Xenopus and sea urchin. The inverted repeats are essentially randomly positioned with respect to both sequence class and sequence arrangement, so that all three sequence classes are found to be mutally interspersed in a portion of the genome.


Nucleotide sequence of human placenta cytoplasmic initiator tRNA. Cytoplasmic initiator tRNA from human placenta has been purified. The nucleotide sequence of this tRNA has been determined and found identical to that of initiator tRNA from mammalian cytoplasm.


The organization of sea urchin histone genes. Sucrose gradient analysis of total sea urchin DNA cleaved with the EcoRI and Hind III restriction endonucleases and identification of histone coding gene sequences by hybridization with histone mRNA have elucidated the basic organization of the histone gene repeat unit. These data, plus results obtained by electrophoretic analysis of purified endonuclease-cleaved sea urchin histone DNA and hybridization with cRNA transcribed from the eucaryotic segment of constructed plasmid chimeras cloned in E. coli, show that the several DNA sequences coding for individual histone proteins are intermingled in a 7 kilobase (kb) repeat unit. Cleavage of total sea urchin DNA with EcoRI produces 2.2 and 4.8 kb fragments, and which are contained in a 7 kb Hind III fragment. Cleavage with both enzymes reveals that the 2.2 kb EcoRI fragment contains a Hind III site 0.15--0.2 kb from an end. RNA.DNA hybridization between chimeric palsmic DNA and purified individual mRNAs isolated from sea urchin embryo polyribosomes has been used to assign coding sequences to either the 2.2 or 4.8 kb region of the histone DNA repeat unit. A map of the histone genes is proposed.


Application of mass spectrometry to the sequencing of proteins.


Treatment of hypoparathyroidism with 1,25-dihydroxycholecalciferol. Five patients with hypoparathyroidism (three post thyroidectomy and two idiopathic) were treated with synthetic 1,25-dihydroxycholecalciferol (1,25-(OH)2-D3) for up to 6 months. In each case daily oral administration of 1 microgram 1,25-(OH)2-D3, either alone or with additional calcium, raised ther serum calcium into the normal range. The serum phosphorus and the renal tubular reabsorption of phosphorus fell during treatment. None of these patients developed hypercalcaemia and no other complications of treatment have been recorded. 1,25-(OH)2D3 seems to represent a significant improvement over conventional methods for treating hypoparathyroidism.


A re-examination of the conformation of secretin in water. An examination of a series of peptides corresponding to partial sequences of secretin and the application of empirical conformational parameters to the sequence has reopened the question about the position of the short helical stretch in the folded chain. In earlier studies, this was tentatively placed near the N-terminus, while newly accumulated evidence points to the C-terminal area. The possible role of ion pairs in the stabilization of the folding was investigated by the preparation and examination of synthetic analogues of the C-terminal tricosapeptide part of secretin. The carboxyl groups of glutamic acid (in position 9) and of aspartic acid (in position 15) were replaced by carboxamides. The 9-glutamine and the 15-asparagine analogues show a significant decrease in helical character. This loss of "structure" is even more pronounced in the 9-glutamine-15-asparagine tricosapeptide. Thus, ion-pair formation is indeed implicated as one of the forces which stabilize the folded conformation of the chain. Possible correlations between biological activity and secretin-like architecture were studied on several smooth muscle preparations.


Regulation of calcitonin secretion in vitro. We have developed methods for the long-term maintenance of monolayer cultures of both human and trout calcitonin-secreting (C-) cells (Roos et al., 1974a, 1975). Secreted calcitonin (CT) can be measured by specific and sensitive radioimmunoassays for human calcitonin (HCT) and trout calcitonin (TCT) (Deftos, 1971; Deftos et al., 1974; Roos et al., 1974b). The combined application of these cell culture and radioimmunoassay methods to the in vitro study of CT secretion has revealed fundamental differences in the regulation by divalent ions of CT secretion in mammalian and nonmammalian C-cells. Our results also indicate a specific role for secretin as a CT secretagogue in trout. In addition to establishing the individual secretory effects of calcium and the enteric polypeptide hormones on the C-cells of both species, we have investigated the combined effects of these agents in both human and trout C-cell cultures. The noncompetitive augmentation of the secretory effects resulting from the actions of specific combinations of these secretagogues in each of these C-cell populations suggests that CT secretion is regulated by co-ordinate interaction of specific ionic and peptide secretagogues. An elucidation of the cellular mechanisms involved in such multifactorial control of C-cell function should result from the continued investigation of CT secretion in these C-cell cultures.


The heterogeneity of gastrin, with reference to conversion of gastrin-17. High-resolution gel chromatography monitored by a sensitive radioimmunoassay for gastrin has disclosed that gastrin circulates in twenty different components. Since the assay is specific for the biological active sequence of gastrin (-Trp-Met-Asp-PheNH2), we conclude that all the twenty circulating molecular forms of gastrin encountered in this study may participate in the regulation of gastric acid secretion. There are two dominating forms of gastrin in serum: gastrin-34-like components and gastrin-17-like components. The former constituted 60% and the latter constitutes approximately 30% of the immunoreactive gastrins. We suggest that extragastrointestinal conversion of gastrin-17, released from tissue, is an important source of gastric-34-like components. Such conversion has been observed in intact man and pig. Studies with the isolated perfused porcine liver indicate that the liver is important in the metabolism of gastrin-17, and that conversion may be a hepatic function.


Skeletal resistance to the calcaemic action of parathyroid hormone in uraemia: a mechanism for the hypocalcaemia and secondary hyperparathyroidism of chronic renal failure.


Structure activity studies on somatostatin. Seven synthetic analogues of somatostatin helped clarify structural requirements for suppression of growth hormone secretion in rats. Size of the ring is not critical; deletions of serine-13, lysine-4 or asparagine-5 result in peptides which retain an appreciable fraction of the activity. The analogue des-Ala1, Gly2, Asn5-somatostatin lowers plasma growth hormone and insulin levels without affecting plasma glucagon levels significantly.


Analogues of human calcitonin. IV. Influence of leucine substitutions in positions 12, 16 and 19 on hypocalcaemic activity in the rat. The replacement of the three aromatic amino acids in positions 12, 16 and 19 of human calcitonin (HCT) by leucine residues, which occupy the corresponding positions, in ultimobranchial, e.g. salmon and eel, calcitonins, increased the hypocalcaemic potency of the peptide, as determined by bioassay on the rat, about 10-fold. The individual substitutions were not all equally augmentative: leucine (12) enhanced the activity of HCT about 4--5 times, but leucine (16) and (19) afforded no increase at all. Combination of leucine (12) with a deamino cysteine at the N-terminus yielded an analogue 10 times more potent than HCT. Another analogue containing valine in position 8 in place of methionine as well as the three leucine substituents in position 12, 16 and 19 proved more active than the tri-leucine analogue, but the additional introduction of tyrosine (22) nearly doubled the hypocalcaemic potency of the latter. The duration of the hypocalcaemic effects of the substituted peptides closely followed the changes in potency.


Hereditary bone dysplasia with hyperphosphatasaemia: response to synthetic human calcitonin. Four cases of familial bone dysplasia with hyperphosphatasaemia were treated with synthetic human calcitonin. Prior to therapy, all four cases were characterized by marked bone deformity, pain, tenderness and elevated levels of serum alkaline phosphatase and urinary hydroxyproline. Treatment with calcitonin produced in each case a striking clinical, biochemical and radiographic remission. Pain and tenderness was greatly diminished and urinary hydroxyproline and serum alkaline phosphatase levels were significantly decreased. Radiographic regression of the bony abnormalities was apparent as early as 4 1/2 months after the start of treatment. Prior to therapy bones exhibit no real organization. After calcitonin treatment, the radiographic appearance of a normal cortex and medullary cavity was clearly evident for the first time.


The regulation of intracellular calcium.


The relationship between oestrogen status and bone loss in post-menopausal women. The relationships between the rate of change of metacarpal cortical width, urinary hydroxyproline/creatinine ratio, net absorption of calcium and maturation value of vaginal smear have been examined in normal post-menopausal women and crush fracture cases. Rapid bone loss, high bone resorption and low calcium absorption are shown to be associated with poor oestrogen status, with crush fracture cases presenting an exaggerated view of the normal post-menopausal situation.


Modes of inactivation of neurohypophysial hormones: significance of plasma disappearance rate for their physiological responses. Analogues of oxytocin and deaminooxytocin with 4-glutamine replaced by 4-glutamic acid methyl ester readily lose their uterotonic activity when incubated with rat serum, presumably by hydrolysis to the much less active 4-glutamic acid derivatives. On the other hand, inactivation of the deaminooxytocin analogue in the rat uterus, as demonstrated by the "oil-bath" technique, is only slightly more rapid than that of deaminooxytocin and distinctly slower than that of oxytocin. Its in situ/in vitro ratio of uterotonic activity is less than 0.1 whereas that for deaminooxytocin is about 3 and also the peristence of the uterotonic effect in situ is slightly less than that of deaminooxytocin. The results with these "rapidly inactivated" analogues can be used as proof of some predictions of the three-compartment model for tissue distribution of neurohypophysial hormones and its influence upon the time course of a biological response published earlier. The potential use of analogues of neurohypophysial hormones as probes for inactivation mechanisms and the results thus far obtained are discussed.


The mode of action of immunosuppressive agents.


Contributions of pressure and flow sensitivity to autoregulation in mesenteric arterioles. The relative influence of presence and flow on dilation of arterioles with pressure reduction was examined in preparations of cat mesentery. Erythrocyte velocity and diameter were measured in individual arterioles during stepwise reduction in mesenteric arterial pressure. Volume flow was calculated from velocity and diameter data. Approximately half of the arterioles which dilated with pressure reduction also showed an increase in volume flow. In a second series of experiments, a graded reduction of flow in single arterioles was produced by local downstream occlusion. Graded occlusion caused dilation. In a third series, flow in single arterioles was completely stopped by downstream occlusion, and arterial pressure was then lowered. Most arterioles dilated with pressure reduction. In a fourth series, flow in the total preparation was completely stopped and static intravascular pressure set by a reservoir. Elevation of static pressure typically produced arteriolar constriction. We conclude from these studies that the mesenteric arteriole is sensitive both to intravascular pressure and flow, with the former probably more important than the latter in the phenomenon of autoregulation.


Maltose ornithine iron medium: a new diagnostic test for Enterobacteriaceae.


Tubuloglomerular feedback: effect of dietary NaCl intake.


Biophysical properties of mycobacteriophages. Biophysical characterization of two mycobacteriophages (Phlei phage and Butyricum phage) was carried out. Biophysical parameters obtained were: (i) buoyant densities in CsCl of I . 51 g/ml for both phages; (ii) sedimentation coefficient of 490 S and 410 S; (iii) DNA content of 42 and 34% and (iv) mol. wt. calculated by electron microscopic dimensions to be 123 x 10(6) and 116 x 10(6) for Butyricum and Phlei phage, respectively.


Mechanism of pellet egestion in great-horned owls (Bubo virginianus). To study the mechanism of oral pellet egestion in great-horned owls, bipolar electrodes and strain-gauge transducers were chronically implanted in the esophagus, muscular stomach, and duodenum of six owls. Recordings from conscious owls plus simultaneous radiographic observations revealed characteristic gastrointestinal motility patterns associated with egestion. Beginning at about 12 min before egestion, gastric contractions formed the final shape of the pellet and pushed it into the lower esophagus. The pellet was moved out of the esophagus by antiperistalsis during the last 8--10 s before egestion. During pellet egestion, contractions of abdominal muscles were not detected. Pellet egestion appears to be unlike either emesis in mammals with a simple stomach or regurgitation in ruminants.


Regulation of avian insulin secretion by isolated perfused chicken pancreas. Chicken insulin secretory responses to glucose, glucagon, tolbutamide, and lack of Mg2+ were measured using isolated perfused in situ chicken pancreata. Although elevating perfusate glucose concentration from 100 to 250 mg/100 ml failed to increase insulin release, 500 mg glucose/100 ml provoked a transient 5-min insulin response. Additionally, 700 mg glucose/100 ml resulted in both a transient response and subsequent elevation in secretory rate that continued throughout the following 50-min stimulatory period. Glucagon (500 microgram/ml) and omission of perfusate Mg2+ potentiated glucose-stimulated insulin output by 6 and 25%, respectively. A faster release of insulin (less than 1 min) occurred during tolbutamide infusion (0.13 mg/ml) than with either 500 or 700 mg glucose per 100 ml (2-3 min); however, secretory rates declined to near basal levels within 5 min. Mammalian-like insulin responses to glucose, glucagon, Mg2+ lack, and tolbutamide suggest similarities between avian and mammalian beta-cell insulin secretory mechanisms. Nevertheless, the relatively high chicken insulin release threshold and low insulin output to glucose indicate that chicken pancreata are relatively glucose insensitive.


Occupational health and the 1975 d.i.h. course.


Acute renal failure.

Acute renal failure.


Blood transfusion and the problem of black donors.


Conversion of 3beta, 15beta, 16beta-trihydroxy-5-androsten-17-one to 1,3,5(10)-oestratriene-3,15beta,16beta,17beta-tetrol by placental homogenates.


Correlation of in vivo and in vitro activities of some naturally occurring androgens using a radioreceptor assay for 5alpha-dihydrotestosterone with rat prostate cytosol receptor protein. A radioreceptor assay for 5alpha-dihydrotestosterone (DHT) which uses castrated rat ventral prostate cytosol receptor and either charcoal dextran or polyethylene glycol to separate unbound steroid is described.  Optimal amounts of charcoal (25%), polyethylene glycol (10%), stability of the receptor, and time after castration were determined to perfect the assay.  The cytosol receptor bound tritiated DHT, which was displaced by unlabeled DHT in a dose-response relationship, with either method of separation.  Testosterone competed for the receptor, but was one-third as potent.  Other active substances were: 4-androstene-3beta, 17beta-diol, 5-androsten-3beta, 17beta-diol, 5alpha-androstane-3beta,17beta-diol, 5alpha-androstane-3alpha,17beta-dio l and 5 alpha-androstane-3,17-dione.  Binding was not inhibited by estradiol, dehydroepiandrosterone, 4-androsten-3,17-dione, progesterone, cortisol, 3beta-hydroxy-5alpha-androstan-17-one, or clomiphene citrate.  Since 4 androstene-3,17-dione and dehydroepiandrosterone are 15 and 12.6% as potent androgens and DHT in vivo, but were inactive in this assay, they are probably metabolized to active androgens in vivo.  The stereospecificity for binding and for androgenic effect of the other steroids are discussed.


Identification of the androstenetriolones and androstenetetrols present in the urine of infants.


Direct determination of steroidal sulfates.


An assessment of the total estrone, estradiol-17beta and estriol in high risk pregnancy plasma.


Studies on the mechanism of testosterone action on glucose metabolism in the rat ventral prostate.


Cervical ribs in black mineworkers.

Cervical ribs in black mineworkers.


The primary, secondary and intensive care of the injured. Valedictory address.


A controlled comparative trial of unilateral and bilateral sinusoidal and pulse E.C.T. in endogenous depression. Sixty-six depressed patients who achieved a score of 6 or more on the Newcastle Scale were randomly allocated to 3 different modes of E.C.T.--unilateral sinusoidal, bilateral sinusoidal and pulse. Outcome, one month after completition of 6 or more E.C.T., was assessed by mean fall in Hamilton's score. Overall, there was a highly significant improvement but among the 3 groups there were no significant differences, findings not accounted for by differences in mean age, numbers of E.C.T. given or mean initial Hamilton's score among the groups. The practical and theoretical implications of the results were discussed. There seems little justification for the continued use of bilateral sinusoidal E.C.T. as opposed to other methods.


Rehabilitation in South Africa and overseas, with special reference to spinal injuries.


Hepatic tuberculosis with haemosiderosis.


Relationship between glycolysis and steroidogenesis in cultured Graafian follicles stimulated by LH or prostaglandin E2.


Oestradiol-17beta, progesterone and 17alpha-hydroxyprogesterone concentrations in jugular venous plasma in cows prior to and during oestrus.


Pathways for in vitro biosynthesis of corticosterone and cortisol in the guinea-pig adrenals.


Androgens in testes and adrenal glands of the fetal pig.


Osteoclastoma--right knee.

Osteoclastoma--right knee.


Metabolism of estrone and progesterone in vitro in human saliva.


The split-brain symptoms of schizophrenia.


Low calorie protein diets.

Low calorie protein diets.


Etiology and the prevention of wars (preliminary thoughts). There is a suggestion in the previous paper that a way out to convey the overwhelming progress and "heavy" civilization of humanity may be to explore the Space, the Universe, and it, indeed, results in an excellent job what American and Soviets together are these days endeavoring toward new exploration in Space. Let us see now, is this way out going to give the solution of our problem, hence avoid wars in the world?


Psychiatry on the prevention of crime.

Psychiatry on the prevention of crime. The main endeavor of Forensic Psychiatry is to find out the causes, to investigate the psychodynamics of crime, hence based on them, to try to prevent or to treat them. In this connection, I will not enter into details today about the position of delinquency into psychodynamics, as they have elsewhere been described, I only would emphasize so-called "hystero-epilepsy" of Charcot.


Visual and auditory cognitive processing affected by epilepsy. Neuropsychological studies of epileptic patients may be expected to reveal specific cognitive dysfunction even in patients with normal general intellectual ability. Difficulties in cognitive processing by visual-spatial and auditory-verbal modes are indicated by this investigation employing the Wisconsin Card Sorting Test and the Token Test, with epileptics of normal intelligence. Impairment in capacity for sustained, focused attention significantly affects performance of epileptic patients on these clinical measures, and others as well which require vigilance and attention and sensory-perceptual discrimination.


Civil background variables significant to the development of psychiatric disorders during military service. The author served as a military psychiatrist for one year. In this article, he presents material from a personally conducted examination of soldiers with psychiatric disorders. He has also collected material on two control groups of soldiers. He has also processed patient material collected by other military psychiatrists. Both patient groups are compared with the two control groups concerning various civilian background factors. The study shows that the patients differ from the controls regarding a number of negative factors in their civilian background. These factors can be seen as predisposing to the development of psychiatric disorders while in the Military. The author concludes that these factors ought to be identified at the conscription examination, which is at present inadequate from a psychiatric viewpoint. An effort should be made at conscription to construct a profile of the individual's adaptability. The possibility of the development of a psychiatric disorder could thus be better evaluated.


Mood studies of alcoholic patients.

Mood studies of alcoholic patients. A series of mood studies of alcoholics before, during, and after participation in an inpatient treatment program is reviewed. Data highlight the extreme affective heterogeneity which characterizes alcoholics.


Inappropriate counter-regulatory hormone levels in insulin treated diabetes. The data herein presented describe, identify, and quantitate interrelationships among blood glucose and serum insulin, growth hormone, cortisol, and glucagon levels of hospitalized insulin-treated diabetic patients. The findings indicate that conventional diet and insulin therapy of diabetes mellitus is almost always accompanied by inappropriate counter-regulation by growth hormone, cortisol, and glucagon. The data are consonant with the hypothesis that a shortage of insulin is only one of the multihormonal defects of diabetes.


Effect of aldosterone on flavin coenzyme biosynthesis in the kidney.


Norethynodrel binding to human plasma proteins.


A study of "palthi" posture among Indians. "Palthi" (leg-folding) posture has been studied in a random survey of 864 right handed and 37 left handed persons, ranging from 5 to 66 years of age and belonging to the Gwalior district of Madhya Pradesh (Central India). The results show that the frequency of L-type "Palthi" (i.e. left leg crossed above the right leg) is significantly greater than that of R-type, and that it is not correlated either with age, sex, or handedness. Since "Palthi" is fairly under voluntary control, the cerebral dominance may be an important factor in the genesis of this posture pattern. This is well in keeping with the revised concept of cerebral dominance.


Preparation of 19-hydroxypregnenolone 3-acetate and 3-methyl ether.


Simultaneous radioimmunoassay of plasma progesterone and 17-hydroxyprogesterone normal values in children, in men and in women throughout the menstrual cycle and in early pregnancy.


Some morphological measures in tubercular patients and their comparison with normals. One hundred and fifty tubercular patients from the Tuberculosis and Chest Diseases Department Medical College, Patiala, were compared with the normal population of the same ages from Patiala district. The tubercular patients and the normal individuals do not differ significantly in height, lower extremity length, bicristal breadth and humerus bicondylar diameter. However, the values of weight, sitting height, biacromial diameter, chest circumference and femur bicondylar diameter are significantly less among the patients. The indices--sitting height/height, lower extremity length/sitting height, biacromial diameter/bicristal breadth--also show significant differences in the physique of the two groups.


Androgen binding sites in testis cell fractions as measured by a nuclear exchange assay.


Metabolism of estradiol-17beta in human endometrium during the menstrual cycle.


A series of optical structural and isomeric analogs of estradiol: a comparative study of the biological activity and affinity to cytosol receptor of rabbit uterus.


Effects of anesthesia, rapid blood sampling and castration on testosterone and luteinizing hormone levels in the adult male rabbit.


Progesterone-binding protein in human myometrium. Binding site concentration in relation to endogenous progesterone and estradiol-17beta levels.


Steroid transformations in porcine salivary glands.


Foot and mouth disease in Nigeria.

Foot and mouth disease in Nigeria.


Avian encephalomalacia in Uganda.

Avian encephalomalacia in Uganda.


The incidence of rabies in animals in the Sudan.


Rabies in Egypt: biological and antigenic properties of four viruses recovered from nonclassical rabies cases.


Rabies in Egypt: a report of four cases showing some deviation from classical rabies.


In vivo metabolism of 3H-dihydrotestosterone and 3H-androstanediol in adult male rats.


Antibiotic sensitivity of Staphylococcus aureus isolated from mastitis in cows at Benghazi localities.


Animal brucellosis in Kenya: a review.

Animal brucellosis in Kenya: a review.


Salmonella contamination in a meat-meal processing plant in Tanzania.


Use of cambendazole for treatment of calves infected with Cysticercus bovis.


A micromethod for measuring the activation of estradiol receptors of the rat uterus.


A comparative toxicological and biochemical study of the effects of coumaphos on larvae of Haemaphysalis longicornis and Boophilus microplus.


The helminth parasites of the helmet guinea fowl (Numida meleagris galeata pallas) in Ghana.


The Tabanidae of Bahr El Arab, Southern Darfur district, Sudan.


Haemorrhagic syndrome of chickens: a case report.


Circular dichroism of bovine serum albumin--testosterone systems.


Differential drug therapy of the hyperlipoproteinaemias. The efficacy of lipid lowering drugs alone and in combination. In a trial of lipid lowering drugs a 6 week course of treatment gives reasonably reproducible post-treatment values. Comparison of serum cholesterol and triglyceride levels after treatment should be preferred to percentage reductions, in both individual patients and groups of patients. Therapy with clofibrate combined with Bufor gives lower post-treatment values than does either drug alone. Cholestyramine drugs like Cuemid and Questran give post-treatment cholesterol values which cannot be predicted from the result of clofibrate treatment alone.


Lipids and heart disease. Preventive aspects.


Treatment of children with familial hypercholesterolaemia.


Reflections on the past decade in health manpower.


Nurse practitioners in primary care: the southern Ontario randomized trial.


Health care trends and nursing roles.

Health care trends and nursing roles.


The quality of general medical services by dentists.


Private nursing practice: some facilitators and barriers in health care.


Health care delivery systems for tomorrow: possibilities and guidelines.


Health policy and the HMO.

Health policy and the HMO.


Physical growth in Delhi children: some observations.


Nurse practitioners in primary care: the McMaster University educational program.


Cancer biology. II. Etiology and therapy.


Capital projects.

Capital projects.


Bioethical questions related to hepatitis B antigen.


Mine water health hazards.

Mine water health hazards.


Severed flexor tendons of the index finger.


Keeping safe water safe.

Keeping safe water safe.


Recent developments in automatic refraction. A detailed description of the Ophthalmetron is given. This is an automatic refractometer using the principle of infra-red retinoscopy and having a sensitivity range from -18.00 D to +17.00 D with a resolution of 0.25 D. By means of a rapid light scan across the patient's pupil the instrument determines the direction of motion of the retinal reflex in all meridians and a movement null-point is determined electronically. Results are then plotted automatically on a chart drum. Some early validation studies have shown the Ophthalmetron to provide a refraction end-point in good agreement with conventional retinoscopic findings. The paper concludes with an appendix in which the features of three commercially available automatic refractometers are compared.


Stereopsis measured by random-dot patterns--a new clinical test. Comparative measures are presented of stereo-acuity by random-dot stereograms using a phase difference haploscope and a Titmus stereogram. Using two groups of fifteen subjects each, one with full binocular vision and the other of small-angle strabismic amblyopes, greater discrimination between subject groups was found with the random-dot stereograms. It is concluded that random-dot stereograms produced by crossed Polaroid would be a more effective clinical means of determining stereo-acuity.


Survey of anaesthetic practice.

Survey of anaesthetic practice.


The effect of pilocarpine on the ACA ratio. The results of an experiment on ten subjects of the combined effects of pilocarpine and phenyleprine on the accommodation/convergence association (ACA) ratio. Using the gradient test method a significant decrease in the ACA ratio was found at the p=0.01 level. A physiological basis for this effect is hypothesized.


Miliary pulmonary nodules--unknown aetiology.


Visual acuity and colour vision tests--a preliminary report. Results are reported of a preliminary survey of colour vision changes in fifteen patients with central serous retinopathy. Colour vision was monitored with the HRR plates, 100-hue test and Nagel anomaloscope. In those patients revealing an acquired dyschromatopsia the defect had a tritan-like response. However, diagnosis is made difficult because of the positively correlated trend between 100-hue error score and visual acuity.


The design of diagnostic tests for defective colour vision. Pseudoisochromatic plates are among the most popular tests for defective colour vision. They are particularly good for screening but are less good in assessing the degree and type of the colour vision defect. To select colours for use in diagnostic plates a large number of colour defective subjects have made colour matches with the Lovibond Tintometer and the isochromatic data collected. Pseudoisochromatic plates have been printed using pairs of colours only and incorporating both a random dot and a regular dot format. These plates have proved effective in a clinical trial. Not only must pairs of inks be carefully selected to lie upon appropriate isochromatic lines but the luminance contrast between the two colours must be kept within 5%. Failure to control luminance contrast is as much a source of error in currently available pseudoischromatic tests as the inappropriate use of colour.


Some aspects of trauma at Baragwanath hospital.


Eye movements in heterophoria.

Eye movements in heterophoria. Reference is made to earlier reports by the author in which versional recovery eye movements were observed during the cover test in the non-occluded eye in case of heterophoria. This provided support for Hering's Law, although the magnitude of the versional movement did not relate to the degree of heterophoria. Recent studies of eye movements during the cover test on very small heterophorias have suggested there is a threshold value below which these versional recovery movements are absent, and that is presence depends on such factors as the size of the phoria, the degree of ocular motor dominance and patient concentration.


Changes in mining medicine on the South African gold mines. Valedictory address.


Fractures of the upper surface of the tibia.


Landry-Guillane-Barre syndrome.

Landry-Guillane-Barre syndrome.


Cancer biology. I. Induction, regulation, immunology and therapy.


Latency in herpex simplex infections.

Latency in herpex simplex infections.


Structural and functional analysis of ribosome subunits isolated from mouse liver following a toxic dose of dimethylnitrosamine. Ribosomal subunits, isolated from mouse liver after a single dose of 50 mg/Kg b. w. of DMN, have been tested for their structural and functional properties. CsCl analysis has shown that after DMN treatment no loss of structural proteins occurs in both 40 S and 60 S ribosome subunits. It has been also demonstrated that these subunits are able to synthetize in the poly-U programmed system at low Mg++ concentrations, in the presence of heterologous initiation factors, at the same efficiency as the corresponding controls.


Responses of simple and non-oriented cells to stimuli of variable duration in areas 17 and 18 of cat visual cortex. Single unitresponses to photic stimuli of variable duration were studied in the cat visual areas 17 and 18. The closed-chamber technique was used to record extracellularly impulsive electrical activity in locally anaesthetized, Flaxedil-paralyzed and artificially ventilated cats. Stimulus duration ranges between 0.020 and 10 sec, the background luminance and the intensity of stimuli being constant. We found a first group of cells which fire independently of the stimulus duration; a second group which fire in close relation to the stimulus duration in a rather wide range of values, and a third group which fire monotonously as long as the stimulus goes on. The three groups of cells are supposed to be three functionally different types of cortical cells.


Approaches to antiviral chemotherapy: a status report.


Enzyme engineering and development.

Enzyme engineering and development.


On the mechanism of herpes virus DNA replication and the genesis of defective particles.


Regional application of enzyme technology-report on.


Enzyme engineering: introduction.

Enzyme engineering: introduction.


Applications of immobilized biochemical systems in India and other developing countries.


Preparation and utilization of immobilized enzymes: theoretical and practical aspects.


Photosynthesis and solar energy conversion.


Chloroplast membrane organization at the supramolecular level and its functional implications.


Energy distribution in the photosynthetic apparatus of plants.


Models for reaction-center and antenna chlorophyll.


Electron transfer reactions and photoexcited porphyrins.


The reaction center of photosystem II.

The reaction center of photosystem II.


Structure of a bacteriochlorophyll a-protein from Prosthecochloris aestuarii.


Chlorophyll-proteins from Acetabularia mediterranea.


Dynamic interactions among structural components of the chloroplast membrane.


Phycobilisomes in relation to the Thylakoid membranes.


Models of pigment aggregation based on crystallographic investigations: recent results pertinent to the composition of the light-harvesting system of green sulfur bacteria.


Chlorophyll-proteins, reaction centers and photosynthetic membranes. Abstracts of poster discussions.


Parkinsonism.

Parkinsonism.


Development of the upper respiratory system.


Glaucomas. Diagnosis and management.

Glaucomas. Diagnosis and management.


Motor neurone disease.

Motor neurone disease.


The pill and other drugs.

The pill and other drugs. Drugs may interact with oral contraceptives by influencing their absorption, stimulating or inhibiting their metabolism, or acting through the same or physiologically opposing receptor mechanisms. Breakthrough bleeding and spotting are the warning signs that should encourage enquiry about additional drug taking. The use of powerful enzyme-inducing drugs like phenobarbitone or rifampicin, effectively rules out the use of oral contraception as a reliable method of fertility control. The problem of drug interaction with oral contraceptives should be kept in perspective; the number of reports is still very low.


Everest south-west face.

Everest south-west face.


Ultrasound and the liver.

Ultrasound and the liver.


Genetic blood disease in immigrants.

Genetic blood disease in immigrants.


Respiratory manifestations of disease of the central nervous system.


The management of depression.

The management of depression.


Drug treatment of spasticity.

Drug treatment of spasticity.


An understanding of breathlessness.

An understanding of breathlessness.


The microsurgical treatment of labyrinthine vertigo. In summary, (1) the fact that VNS spares auditory function makes it preferable to labyrinthectomy. A comparison of VNS and the "Conservative" operations on the inner ear shows that they have much the same effect on hearing. VNS by controlling vertigo may also provide an additional benefit by eliminating one of the stress factors concerned in the perpetuation of hydrops. (2) In the treatment of vertigo, VNS is now the most effective method of treatment. Because the elimination of vertigo is what chiefly concerns most of our patients, we suggest that when medical treatment and surgical decompression of the saccus endolymphaticus fail to control vertigo, then VNS is the surgical method of choice.


Bullet and bomb injuries.

Bullet and bomb injuries.


Computed tomography in the evaluation of malignant disease.


Megaloblastic anaemia.

Megaloblastic anaemia.


Tropical enteropathy.

Tropical enteropathy.


Travellers diarrhoea.

Travellers diarrhoea.


Growing points in renal disease.

Growing points in renal disease.


Giddiness.

Giddiness.


Acupuncture.

Acupuncture.


Looking around in cardiac surgery. Lettsomian lecture II 1977.


Medical aspects of liver disease. The role of liver biopsy.


Electrophysiologic assessment of the lower portion of the auditory pathway in the human subject. A preliminary report on auditory evoked eighth nerve and brain stem potentials including their relation to the electrocochleogram. The electrophysiologic approaches to the assessment of electrical activity of the various portions of the human auditory pathway are reviewed. A method of detection of click evoked eighth nerve and brain stem responses with the use of surface electrodes is described. Preliminary observations in the normal subject with different stimulus parameters and in patients with unilateral deafness are presented. Simultaneous recordings of the eighth nerve and brain stem responses with the cortical evoked response and with the extratympanic electrocochleogram are described. The technique of click evoked eighth nerve and brain stem potentials offers us an additional method of objective assessment of auditory function in a human subject.


Cochlear and vestibular dysfunction resulting from physical exertion or environmental pressure changes. Inner ear damage due to physical exertion or environmental pressure changes has been described only recently. According to Goodhill there are two possible mechanisms of injury: the explosive and the implosive. The former is triggered by increased C.S.F. pressure transmitted to the perilymph space; the latter by increased pressure in the middle ear transmitted to the perilymph space through the two windows. Sudden hearing loss or dizziness, or both, are the main symptoms. The diagnosis will be established by the history, the audiogram, and the electronystagmogram (ENG). The treatment is still controversial. As the formation of a fistula is a real possibility, an exploratory tympanotomy is suggestive on suspected cases. Due to the variety of symptoms every case has to be assessed individually. A detailed history is of utmost importance.


The use of electro-acoustic impedance measurements in detecting early clinical otosclerosis. The first evidence that sodium fluoride (NaFl) can stop the otosclerotic process was recently presented. This development has placed new emphasis on the early detection of clinical otosclerosis. Electro-acoustic impedance measurements often detect minute changes in absolute impedance and compliance of the ossicular chain. The most valuable diagnostic information, however, is a negative on-off (biphasic) type of acoustic reflex. These results are often evident prior to the detection of positive clinical signs of otosclerosis. The negative on-off acoustic reflex is reviewed in this paper along with case discussions involving medical/surgical management of early otosclerosis.


Osteogenesis imperfecta of the stapes: an histological study. Two cases of Van der Hoeve's Syndrome are presented with histological findings consistent with the diagnosis of osteogenesis imperfecta. These findings support the view that otosclerosis can be clearly differentiated histologically from osteogenesis imperfecta. Pre-operative impedance studies are recommended on each patient with osteogenesis imperfecta as a useful diagnostic adjunct to determine whether the conductive component in the hearing loss is secondary to stapedial crural deformity and/or footplate fixation.


Meningitis following stapedectomy: its occurrence in the immediate postoperative period. Meningitis following stapedectomy occurring in the immediate postoperative period has not been previously described. This case report documents a case of diplococcus pneumoniae meningitis occurring within 36 hours following surgery with survival of the patient.


Cochleo-vestibular manifestations of jugular foramen pathologies. Patients presenting pathologies of the jugular foramen are presented, for whom the main symptoms are of cochleo-vestibular nature. A classification of the various pathologies is attempted, the anatomy reviewed and hypotheses capable of producing the symptom elaborated.


Click evoked eighth nerve and brain stem responses (electrocochleogram)--experimental observations in the cat. The origin of waves two to five occurring in the first 10 milliseconds after repetitive click stimuli is not definitely established. Published studies have suggested that waves two and three are derived from the cochlear nucleus and superior olivary complex respectively and that waves four and five are generated by the inferior colliculus. Our studies of the effect of chronic brain stem lesions in the cat do not support this hypothesis of specific localization, but reveal that a more general reduction in amplitude, and at times prolongation in latency, occurs with lesions in the pons and lower midbrain and that wave one amplitude is also diminished with brain stem lesions.


Replication patterns in polytene chromosomes of Drosophila hydei, Drosophila neohydei and their hybrids.


The extrathoracic airways.

The extrathoracic airways. Evidence is presented in support of the view that the extrathoracic airways are more than an anatomical pathway, and that they play an important and dynamic role in respiratory upper and lower respiratory tracts are cited. The importance of rhinomanometry to functional nasal surgery is recognized and it is advocated that tests of extrathoracic airway function be further developed and standardized and that they be taken into consideration together with pulmonary tests function. Examples of pathophysiological relationships between the in the assessment of respiratory function.


An acrylic dental protector in peroral endoscopy. A simple acrylic dental prosthesis is described which achieves successful dental protection during peroral endoscopy. This prosthesis is not advocated for all cases, but certainly is well warranted for those patients whose upper dentition is at risk during endoscopy because of the prominence of the incisors, the presence of loose teeth, or with extensive restorative work. In present experience, approximately 10 per cent of patients undergoing endoscopy have such an acrylic prosthesis prepared pre-operatively. The commercially available flexible plastic dental guard is used in approximately 25 per cent of cases, and the remaining cases need no specific attention. It is felt that this prosthesis could be constructed by any dentist conversant with the technique.


Vocal cord fixation in laryngeal carcinoma.


alpha-Level of the chi 2-test to compare two Poisson distributed samples.


The effect of laser irradiation on the regeneration of muscle fibers (preliminary report). The authors studied the process of muscular regeneration on experimental models after simple muscle injury and under the effect of low-energy laser-irradiation. It is stated that the stimulating effect not only accelerated the wound healing, but most likely influenced also the qualitative improvement of regeneration.


Peripheral and gastric fibrinolysis in patients with gastric carcinoma. The fibrinolytic system was studied in blood-samples from gastric and peripheral veins of patients with gastric carcinoma. There was a slight decrease in plasma-euglobulin fibrinolytic activity, a rise of plasma-fibrinogen level and antithrombin VI activity, and a decrease in antiplasmin activity. The changes observed are more marked in blood-samples from gastric veins. Some therapeutical implications and the role of fibrinolytic agents in malignant diseases are briefly discussed.


Effects of arterial or portal ischemia on survival and metabolism of partially and totally depancreatized dogs. The role of arterial blood flow in hepatic metabolic functions was compared to that of portal flow in two groups of totally depancreatized dogs. Survival times and glucose and nitrogen excretion were significantly greater in dogs with ligation of the hepatic artery than in dogs with an Eck fistula. The dogs with ligated hepatic arteries also showed a significantly slower rise in plasma ketones. The course of diabetes was compared in three additional groups of partially depancreatized dogs consisting of a) dogs with ligated hepatic arteries, b) dogs with Eck fistulas, and c) controls. Hepatic arterial ischemia: 1) increased survival, without insulin treatment (a--650, b--167, c--124 days) 2) did not decrease tracer-determined rate of glucose production 3) led to a greater urinary excretion of glucose, ketone bodies and nitrogen than portal ischemia. Partially depancreatized dogs with either arterial or portal hepatic ischemia maintained a high rate of glucose disappearance on acute deprivation of endogenous insulin (clamping of vessels of their pancreatic remnant) due probably to decreased insulin degradation by the ischemic liver. The dogs died in coma after losing all fat depots. There was severe fatty change in the livers of dogs with hepatic artery ligation, slight in those with Eck fistulas and no fat in the livers of controls.


Activity of catecholamines inactivating enzymes in heart muscle during anoxic cardioplegia in cardiosurgical patients. On the model of anoxic cardioplegia in cardiosurgical patients the influence of anoxia on the activity of monoamine oxidase (MAO) and catechol-O-methyltransferase (COMPT) in right auricular tissue was studied. It was found out that the activity of COMT does not change during anoxia, the activity of MAO considerably increases. With regard to catecholamines stimulating effect upon cardiac metabolism as well as to their calorigenic and oxygen wasting effect the raise of MAO activity may be regarded as favourable phenomenon, because the hastened degradation of released catecholamines at least to a certain degree diminishes the disproportion between oxygen supply and actual oxygen need of the heart and by means of that mitigates the anoxic damage of myocardium.


Misuse of analysis of covariance in aging research and some partial solutions. Gerontological researchers are reminded of the inappropriateness of analysis of covariance wherein the covariate is correlated with the independent variable, typically age. Two partial solutions are suggested that deal with the confounding of age effects with such uncontrolled variables as, for example, initial ability level or level of education.


Some methodological problems in age comparisons of EEG sleep patterns for C57BL/6J mice. Some methodological problems encountered in age comparisons of EEG patterns are discussed in the context of an analysis of open field activity scores before and after preparation for, and recording of EEG sleep patterns. Plasma corticosterone levels 11 days after surgery for EEG electrode implantation were also measured. Activity levels were modified more for three older than for a younger group of animals following surgery/recording sessions. Corticosterone levels were appreciably elevated for the oldest group of animals. The possibility was raised that procedures designed to allow recovery from post surgical shock and adaptation to electrode connections may result in disproportionate differences across age groups.


Effects of immobilization stress on open field behavior and plasma corticosterone levels of aging C57BL/6J mice. Four age groups of C57BL/6J mice (2.2, 6.2, 12.0, and 23.3 months) were subjected to either immobilization or handling (control) procedures. Open field behavior was observed before and after experimental treatments and plasma corticosterone levels were assessed 11 days following the immobilization or handling procedures. Eleven days following immobilization elevated corticosterone levels were observed for all but the 12.0 month group of mice. No behavioral effects were observed for the experimental groups, although both locomotor activity and exploratory behavior declined with advancing age. The age-related decrease in activity was entirely accounted for by scores on the initial open field test. Exploratory behavior was observed to be a more complex function of both age and experience.


Age differences in backward dichoptic masking. An investigation of age differences in backward dichoptic visual noise masking was carried out with young, middle-aged and old subjects. Older subjects were found to be significantly more susceptible to the backward masking effect over longer delays between the target and masking stimuli. These results indicate a decrease with age in the rate at which stimuli can be cleared through the central mechanisms concerned with perceptual processing. These data appear to support the "stimulus persistence" and "arousal" models of aging.


Perceived latitude of choice of institutionalized and noninstitutionalized elderly women. An Importance, Locus and Range of Activities Checklist, designed to assess self-perceived latitude of choice, a self concept and a life satisfaction scale were administered to 25 institutionalized and 25 noninstitutionalized elderly females. The checklist contained statements about 37 activities of daily living which subjects rated for personal importance and degree of choice available to them. The derived latitude of choice score was based jointly on importance and choice ratings for each activity. Institutionalized respondents, living in a relatively restrictive environment, earned significantly lower latitude of choice scores than did noninstitutionalized subjects. Latitude of choice, self concept and life satisfaction scores were significantly correlated. These findings attest to the appropriateness of the technique used to assess perceived latitude of choice. Modifications of the Importance, Locus and Range of Activities Checklist designed to provide a more profound measure of latitude of choice and to improve the applicability of the scale to different categories of respondents were discussed.


Age differences in evoked potential correlates of a memory scanning process. Elderly subjects when compared to young, mature subjects in a memory-scanning task were shown to have longer response times (RT). A late positive component (LPC) of the average evoked potential did not reflect this difference between groups. However, in both groups, the LPC was seen to occur with longer latency at RT increased. LPC amplitudes also declined with increasing RT. There were greater differences between left- and right-hemisphere LPC amplitude and between LPC recorded along the midline scalp in the younger group than in the older subjects.


Effects of genetic and nutritional factors on post-natal reflex and behavioral development in the mouse. The development of a number of reflex activities, of the electrocorticogram (ECoG), and measures of brain and body weight were assessed at different ages from birth to maturity in mice belonging to Swiss-Webster stock and two inbred strains (C57BL/6J and SEC/1Re). The animals also were tested at 60 days of age for exploratory activity and avoidance behavior. These effects of postnatal undernutrition on the measures of reflex and ECoG activity, brain and body weight and avoidance learning were determined at different ages after birth and in adult animals following dietary rehabilitation. Undernutrition during suckling period was introduced by increasing the litter size from 4 pups to 8 or 16 pups per litter. The results indicate that early malnutrition delayed the development of the reflex and ECoG activities and permanently altered brain growth and avoidance learning. The effects were different, depending on the genotype considered. The data indicate that strains characterized by a slower development of brain function do not show early signs of brain malfunction following undernutrition.


The effects of subjects' age and expectations regarding an interviewer on personal space. How close individuals seat themselves to an interviewer was studied as a function of subjects' age and expectations regarding the interviewer. An experimental approach situation was used and the subjects were 10 college students and 10 residents of a county home. Those subjects who had been told to expect an unpleasant interaction seated themselves significantly farther from the interviewer than did those who expected a pleasant interaction. Also, the older subjects seated themselves significantly farther from the interviewer than the younger subjects did. The difference between the age groups was minimal when the subjects' expectations were positive, but was pronounced when the subjects' expectations were negative. The results appear to reflect a greater susceptibility among older individuals to feeling threatened and ill-at-ease in an interpersonal situation.


Experimental unconfounding of age and maternal responsiveness in rodents: prenatal CER conditioning to neonatal ultrasounds. Prior to mating, virgin female Long-Evans Rattus norvegicus received 30 classical CER trials with shock as the UCS and either taped neonatal ultrasounds or a belltone as the CS. Additional animals were placed in groups controlling for exposure to shock, the ultrasounds, the belltone, and placement in the conditioning apparatus. Following mating and parturition mother-infant observations revealed that those mothers previously conditioned to neonatal ultrasounds spent more time in the lactation posture than did mothers from the other groups. The results suggest that naive mothers can be "primed" to exhibit greater maternal responsiveness than is characteristic of primiparous mothers.


Organization and recall of related sentences by elderly and young adults. Differences between elderly and young adults in storage organization of sentence material were investigated using a modification of Mandler's (1967) sorting technique. The elderly were expected to be relatively poor at sorting organization, and would then recall less sentence material. The age groups were also compared on time taken to organize the sentences, recall organization, and performance on a short sentence comprehension test. There were no age differences in conceptual type of organization or clustering of sentences in sorting groups and recall storage organization was not related to the amount of recall. The elderly adults did require more time to acquire the sentences, and had lower recognition test scores. It was concluded that the sorting technique and measures related to it failed to detect the elderly adults' apparent difficulty in acquiring the sentences.


Age differences in life satisfaction as a function of discrepancy between real and ideal self concepts. Six age groups were compared with respect to discrepancy between real and ideal self concepts and the relationship between life satisfaction and discrepancy in self concepts. The subjects, ranging in age from 20 to 75 years, completed two self descriptive adjective rating scales which assessed real and ideal self concept and a life satisfaction index. Significant differences were found among age groups for real and ideal self concepts, life satisfaction, and discrepancy between real and ideal self concept scores. Age was significantly correlated with real and ideal self concept, discrepancy between real and ideal self concepts and life satisfaction. Life satisfaction was significantly correlated with real self concept and discrepancy between real and ideal self concepts. Trends in the data suggested that the younger subjects (20-25 years) had lower real and ideal self ratings and were less satisfied with their lives than the elderly subjects (60-75 years).


Age-dependent changes in hippocampal theta. The hippocampal theta rhythm in rats exhibited an age-dependent slowing in modal frequency. However, the theta rhythm of young rats with chronic respiratory infections showed a similar slowing. Respiratory disease-free hamsters also exhibited an age-dependent slowing of modal frequencies. These findings emphasize that the health status of the animal must be examined carefully in order to establish an age-dependent slowing in hippocampal rhythm.


Regional brain catechol-O-methyl transferase: age related differences in the mouse. Activity levels of COMT were measured in the frontal cortex, hippocampus, hypothalamus and amygdala of two strains of male mice, C57BL/6J and DBA/2J, at various ages between 2 and 30 months. Determinations were made in mice housed under normal conditions and in mice exposed to a form of mild stress, an open-field apparatus, for 5 minutes. There were no major significant differences between the two strains as a function of age. However, after the open-field experience, C57BL/6J mice appeared significantly more responsive to the environment, as interpreted by increases in COMT, than DBA/2J mice. Interpretation is offered that C57BL/6J mice possess a more labile norepinephrine system, in spite of aging, than DBA/2J mice.


Behavioral differences in young and aged mice: strain differences for activity measures, operant learning, sensory discrimination, and alcohol preference. Young and aged mice of the A/J and C57BL/6J inbred strains were given a series of behavioral tests which measured activity of various kinds, emotionally, operant learning, sensory discrimination, and alcohol preference. In general, compared with young mice, aged mice were less active, similar in emotionally, learned the operant discrimination as well, but extinguished more slowly, had poorer discrimination for sweet and bitter taste stimuli, and preferred alcohol to a lesser degree. Strain differences were obtained for most of these tests, and age-strain interactions occurred frequently indicating that behavioral changes occur at different rates for groups which differ in genetic constitution.


Slowing of response time in young and middle-aged hypertensive patients. Reaction time (RT) was compared in 160 hypertensive and 43 normotensive adults. After being screened for coronary and cerebrovascular disorders, each subject completed 12 variations of a serial RT task. Subjects were blocked into normotensive, nonmedicated hypertensive, and medicated hypertensive groups, and into three age groups: 18-31; 32-45; 46-59. Response slowing was observed with increasing age. Significant slowing was also seen in the medicated but not the nonmedicated hypertensive group. Plasma renin activity (PRA) interacted with the medication factor; longest RTs were obtained for treated subjects with low and normal PRA and untreated subjects with high PRA. These results were interpreted in terms of changes in ability to autoregulate cerebral blood flow as a result of vascular damage.


The ontogeny of ultrasounds in two strains of Rattus norvegicus. The developmental course of ultrasonic signaling by neonatal rat pups (Rattus norvegicus) was monitored under varying degrees of hypothermic stress. Signal rate, peak frequency, duration, and complexity of signals were analyzed separately for two different laboratory stocks. Sprague-Dawley and Wistar/Furth. Additionally, preliminary data were obtained on maternal retrieval latencies during the 1st 4 days post-partum and compared with the emission rate of neonatal ultrasounds. On the basis of these and other data, there is evidence that mothers play an important role in mediating many early experience effects. The data suggests that rate of neonatal ultrasounds may provide an index of some aspects of rat of maturity; that there may be different periods of maximum sensitivity to external stimulation in various stocks of laboratory animals; and that differential maternal responsiveness in different stocks of laboratory animals may correlate with differences in neonatal ultrasonic calling. Implications for comparative, genetic, and developmental studies are discussed.


Item recognition following a multiple-item study trial for young and middle-aged adults. The presence study contrasted young and middle-aged subjects on two components of a recognition memory task (a modified verbal discrimination task) that differed in level of difficulty. No age decrement was found for the easier component requiring discriminability between the oldness and newness of items. In addition, age was not a factor in determining the effects of prior relevance or irrelevance of item information on old-new discriminability. An age decrement was found for the more difficult component requiring discriminability in terms of an old item's prior function in the study list. The decrement occurred for the identification of prior function of both right and wrong items.


"Spontaneously" hypertensive mice: a potential genetic model for the study of the relationship between heart size and blood pressure. Mice selected genetically for high and low blood pressure (BP) were compared with regard to heart weight and heart/body ratios. Two experiments were performed with mice ranging in age from 1.3 to 9 months and 11 to 23 months respectively. In a third experiment C57BL/6J mice were compared to the high and low BP mice. Heart/body ratios and heart weights, adjusted fo body weight via covariance analysis, were significantly greater for the high BP mice, but no Age x BP Genotype interaction was observed. Results were discussed in terms of a possible relationship between heart weight and BP via genetic linkage or pleiotropy. The possibility was also raised that compensatory mechanisms for high blood pressure, e.g., cardiac hypertrophy, may operate very early in development for animals who are hypertensive by virtue of selective breeding for blood pressure extremes.


The work-satisfaction, retirement-attitude typology: profile examination. After a typology is developed based on work satisfaction and attitude toward retirement, profiles are examined of respondents appearing in four typological categories. Data at three points in time (1964, 1966, 1974) from 1,922 older (50+) employed males suggest that membranes of some types will be more susceptible to negative consequences of life-cycle change (work to retirement) than others. Findings from socioeconomic status, age, social participation, health, community, housing, family, work, morale, and longevity variables indicate pre-retirement planning approaches should be developed for those having different work-retirement attitudes.


Hemisphere-hand interactions for the matching of letters. Young, middle aged, and older subjects were given a Posner-type physical-name matching task, in which stimuli were presented to one or both hemispheres, and a simple discrimination reaction time (DRT) task. Reaction time differed between young and middle aged groups for both tasks with shorter RT for the DRT task. Reaction time for the older group did not differ significantly from that of the middle aged group for either task, although the reaction time for the older group was longer. Reaction time was faster for all age groups when Stimulus 1 and Stimulus 2 were presented to different hemispheres for the matching task than when both stimuli were presented to the same hemisphere. Ipsilateral finger responses (hand relative to hemisphere) were slower than contralateral responses but this was true only with regard to the visual field of presentation for stimulus 2. These data indicate that sharing of information between hemispheres under certain circumstances increases the efficiency of information processing relative to that observed with unilateral visual half-field (hemisphere) presentations and that hand or finger response relative to visual field of presentation is an important factor which must be controlled and examined relative to visual field of presentation.


Structure of the self-concept from adolescence through old age. The continuity over the major portion of the life-span (ages 9-89 yrs.) of the connotative structure of the self-concept was examined by factor analysis of a semantic differential instrument and found to be essentially constant. Significantly age and sex differences in scores on the four components (Achievement/Leadership, Congeniality/Sociability, Adjustment, and Masculinity-Femininity) emerged and were interpreted.


An analysis of the effect of age on respiratory and digestive functions of the rat. There is little published information on the pulmonary function of aged rats. The data available indicate that the elastic properties of the lung-thorax system of the rat changes with age in a fashion similar to that seen in the case of man and other mammals. However, unlike the situation observed with man, neither the internal surface area of the lungs nor the diffusing capacity of the lungs declines as the rat ages. The diffusion O2 from blood to tissues does not seem to decline with age in the rat and the utilization of O2 by the tissues is either not affected by old age or affected in a subtle fashion. There is no information on how old age influences either the motility or the secretory activity of the exocrine glands of the alimentary tract of rats. There is abundant evidence to indicate that the processes involved in the absorption of dietary carbohydrate and protein are not adversely affected by old age in rats. The data on the effect of age on the enzymatic digestive process are not totally clear, but it seems unlikely that if such changes do occur that they are of a magnitude to seriously hamper the digestive function of the senescent rat.


The total time principle as a substitute for the pacing variable in paired-associate tasks with the aged. Information provided by Monge and Hultsch in support of the pacing variable was reinterpreted i terms of the total time principle. The re-analysis of the transformed data suggests that the total time principle provides a more parsimonious explanation of the data.


Assay for the two different types of lymphocyte complement receptors. This paper describes the assay system for two different types of lymphocyte complement receptors, the immune adherence receptor (C3b receptor) and the C3d receptor.


Audience awareness and catharsis in drama.


Influences on choice of infant feeding methods.


An endonuclease from calf thymus which introduces a limited number of single strand breaks into DNA.


The effect of formaldehyde on the toxicity and potency of pertussis vaccine. Storage of concentrated suspensions of Bordetella pertussis organisms at 5 C for long periods of time in the presence of 0.02% thimerosal as well as heating at 56 C for 30 minutes cannot be relied upon to detoxify the vaccines. Our data show that the toxic thimerosal-killed vaccines can be rapidly detoxified by adding formalin to a final concentration of 0.025-0.05% and incubating at temperatures of 5-20 C for from one to three months. These formalin-detoxified vaccines are protective and meet present potency requirements for pertussis vaccine. Addition of larger amounts of formalin at higher temperatures may detoxify the vaccine but may also reduce the protective effect.


Alexithymia, 'pensée opéatoire' and predisposition to coronopathy. Pattern 'A' of Friedman and Rosenman. The 'behavioral pattern A' and the 'B' pattern in selected groups of male populations in the framework of a prospective and predictive study of a large population of civil service employees is compared. The Personality Inventory of Lazare, Klerman and Armor and the auto-questionnaire of Bortner were used to differentiate the 'A' and 'B' group populations. Selected cards from Murray's TAT seem more appropriate in this respect and demonstrate the lack of efficiency of certain questionnaires in areas of psychosomatic research. Moreover the selected TAT card responses provide some evidences of 'pensée opératoire' or alexithymia in 'A' group subjects whatever its metapsychological implication.


Tentative method of analysis for formaldehyde, acrolein and low molecular weight aldehydes in industrial emissions on a single collection sample.


Cryostat technique for central nervous system histofluorescence. This paper offers a technique for obtaining monoamine histofluorescence in the CNS by means of formaldehyde perfusion followed by cryostat sectioning. No freeze-drying is involved. Cryostat sections are exposed to formaldehyde vapor to complete the fluorophore formation. The fluorescence thus obtained is bright, well localized, and does not require loading the animals with precursors. The anatomical distribution of the pathways is identical to that obtained with the classical technique. Furthermore, the fluorescence is reversible by sodium borohydride, and exhibits the expected changes in intensity with pharmacological manipulations. The sections can be exposed to a cold aqueous medium for as long as 15 min with minimal diffusion of fluorophore; this suggests potential for combining monoamine histofluorescence with other visualization techniques.


Production of saxitoxin by cultures of Gonyaulax catenella.


Further evidence for the humoral stimulation of the liver: effect of a pituitary peptide. The capacity of a peptide isolated from hypophysis (HTH) to affect liver function is further supported by the results of pilot experiments on lipid and protein metabolism. Administration of purified peptide to rats induces reduction in plasma proteins and increase in plasma cholesterol with an abnormal free/esterifield cholesterol ratio in liver. Cholesterol in bile and bile volume excretion increase above normal and the concentration of other substances known to be modified by liver activity shows values different to normals.


Semi-quantitative estimation of changes in noradrenaline content and intraneuronal distribution in the rat vas deferens by fluorescence histochemistry. A semi-quantitative histochemical assay for noradrenaline was developed, based on the assumption that the rate of reaction of noradrenaline with paraformaldehyde depends on transmitter concentration. Changes in organ noradrenaline content caused by drugs or cold-stress were associated with similar changes in fluorescence intensity of organ samples taken for microscopy. Differences in the fluorescence intensity of experimental and control tissues were also found when there was no change in total noradrenaline content, suggesting that fluorescence intensity is not a simple function of whole organ noradrenaline content. Changes in the relative fluorescence of experimental tissues with different paraformaldehyde exposures suggested that the intraneuronal distribution of noradrenaline may affect the rate of development of fluorescence. Analysis of the time course of the fluorescence reaction showed that this was best described by the sum of two first-order exponential components of different half-life. Further results suggested that the first, fast component represents vesicle-bound noradrenaline, while the slow component represents extrangranular transmitter.


"Fear death by Water": the boundaries of the self in Jerzy Kosinski's The Painted Bird.


Sterility treatment and oestrus synchronization with EnzaprostR (PGF 2alpha) in cattle.


Othello's occupation: beyond the pleasure principle.


Sex ratio in twin births.

Sex ratio in twin births. 1. Data on more than 2 1/2 million twin births suggest that the regression of sex ratio in twins on maternal age does not decline monotonically like that of singletons, but, like the incidence of dizygotic twinning, seems to rise and then fall with maternal age. 2. Accordingly it is hypothesized that the sex ratio in monozygotic twins is lower than that in dizygotic twins or that in singletons. This would account also for the low overall sex ratio in twins. 3. The data are consistent with the hypotheses that the monozygotic twin sex ratio is constant for all maternal ages at a value of about 0.496, and that the dizygotic twin maternal age-specific sex ratios are the same as the singleton sex ratios for the same maternal ages. 4. The hypothesized low sex ratio in monozygotic twins is reminiscent of that in some congenital malformations: possibly some aetiological factor is common to monzygotic twins and such congenital malformations. 5. It is suggested that the total-birth sex ratio of triplets is underestimated in national vital statistics records.


A genetic analysis of the normal body-height growth and dental development in man. A twin and family study on the significance of genetic factors for the variation in certain new variables of dental and body-height development is presented. Evidence of a rather strong genetic regulation of most of the variables was obtained from the analysis of the twins and sibs. The data on cousins did not allow any definite conclusions, and it was not possible to obtain a parent--offspring material. Therefore, the study did not give any information concerning the relative significance of additive genetic variation.


Changes in eye colour during early childhood: sex and genetic differences.


The inheritance of scholastric abilities in a sample of twins. I. Ascertainments of the sample and diagnosis of zygosity. A sample of 149 twin pairs was ascertained by extracting all pairs of candidates with the same surname, birth date and address from the files of a public examination taken by about two thirds of the 15-year-old cohort in South Australia. Of these there were 39 opposite sex pairs and 100 same-sex pairs whose zygosity was determined by postal questionnaire. This diagnosis was checked and found to be accurate by typing 47 pairs for ten independent genetic markers. Several analyses suggest that the ascertained twin samples is fairly representative of the fraction of the cohort who took the examination so there is no evidence to suggest that between families components of variance will be underestimated for this reason.


Acute effects of calcitonin on osteoclasts in man. To determine the mechanism by which calcitonin acutely decreases bone resorption in man, salmon or human calcitonin was administered intravenously to five patients with Paget's disease of bone immediately after an iliac crest bone biopsy. After 30 min an adjacent bone biopsy was taken and both were evaluated by light and electron microscopy. The results indicated that calcitonin both decreases the number of osteoclasts and alters the ultrastructure of these cells. Thes acute effects of calcitonin on osteoclasts confirm findings in other species and provide evidence in man that calcitonin, at least in part, retards bone resorption by inhibiting osteoclastic activity.


Nucleotide sequence of salmon testes and salmon liver cytoplasmic initiator tRNA. Initiator tRNAs from the cytoplasm of salmon testes and salmon liver have been purified. The nucleotide sequence of these initiator tRNAs has been determined and found identical to that of initiator tRNA from mammalian cytoplasm. The only difference is the extent of modification of the nuceloside located between the dihydrouridine and the anticodon stems. In the salmon tRNAs, this modified nucleoside is predominantly N2N2-dimethyl guanosine, whereas in the mammalian initiator tRNA it is N2-methyl guanosine.


Radioimmunoassay of androstenedione: the steroid molecule as a probe for antibody specificity.


The inheritance of scholastic abilities in a sample of twins. II. Genetical analysis of examinations results. 1. Examination results for 98 pairs of same-sex and 39 pairs of opposite-sex twins were available for analysis as well as measurements of IQ and height for 47 of the same sex pairs. 2. The choice of subjects taken for examination is not influenced by genetic differences within DZ pairs but seems to be mainly determined by between family cultural differences. 3. Neither the sex difference in opposite-sex pairs nor the difference between first and second born twins in same-sex pairs contributes appreciably to within pair environmental variance. However, same-sex DZ pairs are more often found in different school classes than MZ pairs. 4. No evidence for interaction between genotypes and within-family environments was found for any character. 5. The simple environmental model fails to fit the data for English, French, Geography, Mathematics and IQ whereas the simple genetical model fits. For the remaining subjects it is not possible to exclude either hypothesis. The heritablities of the examination performances are of the same magnitude as that for IQ. 6. Sampling inadequacies are revealed in the data for height but there is evidence for a substantial genetics component of phenotype variation. 7. There is evidence for heterogeneity of gene action in the subjects English and Mathematics supporting the view that there are genetically determined specific abilities acting independently of general intelligence.


The ectopic secretion of calcitonin by lung and breast carcinomas. Many patients with advanced non-thyroid malignancies have elevated plasma immunoreactive calcitonin concentrations. Breast and bronchial carcinomas contain immunoreactive calcitonin and an epidermoid bronchial carcinoma has been shown to produce immunoreactive calcitonin in vitro. We have established monolayer cultures of breast carcinomas and eight out of fifteen consecutive carcinomas released immunoreactive calcitonin; some released HCG (human chorionic gonadotrophin) or CEA (carcinoembryonic antigen). In addition, a primary human breast carcinoma has been shown to release and contain calcitonin after being passaged in 'nude' mice over 1 year. Chromatography of extracts and culture media of a bronchical carcinoma demonstrated that, in contrast with the other tumours, it secreted a form or forms of calcitonin having size, charge and immunological differences when compared to calcitonin M. Preliminary evaluation of plasma immunoreactive calcitonin estimations in patients with breast carcinoma showed that twenty-three out of twenty-eight patients with metastatic disease had elevated plasma calcitonin concentrations, whereas only one out of thirteen with localized disease had high levels.


Production and characteristics of highly specific antibodies to aldosterone.


The genetic contribution to coronary heart disease through lipoprotein concentrations. Many factors both genetic and environmental contribute to the liability to coronary heart disease and one of the genetically determined risk factors is the concentration of lipoproteins in the blood. The evidence so far is that serum cholesterol level is polygenically determined, but that in a minority of the population it is determined by a single mutant gene of large effect. There is evidence that the risk of coronary death for affected individuals is very high and that the condition warrants a maximum effort at identification and treatment. For the remainder of the population whose hyperlipidaemia is polygenically determined, the liability to coronary heart disease might be substantially reduced by simple and appropriate environmental measures aimed at reducing the total liability. The measures if correctly applied should effectively improve the population risks of early coronary death.


Current public policy and medical care evaluation.


Eustachian tube function in children.

Eustachian tube function in children. Eustachian tube function of children with bilateral serous otitis media was studied in 14 ears following myringotomy and pressure equalizing tube insertion. Cases with non-Eustachian tube pathology potentially contributing to Eustachian tube dysfunction were excluded from the study. Eustachian tube function was evaluated utilizing an impedance audiometer to document neutralization of positive and negative middle ear pressures. All cases showed persistent tubal dysfunction for up to six months. Partial incomplete neutralization of positive pressure occurred in 64 per cent, but in no case could negative pressure be partially neutralized even when "locking" was relieved with valsalva. Continuous ventilation of the middle ear for up to six months did not allow a return to normal Eustachian tube function. This is extremely effective palliation, and should be recognized as such.


An international classification of the health problems of primary care.


Behavioral characteristics of C57BL/6J. DBA/2J, and B6D2F1 mice which are potentially useful for gerontological research. This review summarizes the behavioral characteristics of C57BL/6J, DBA/2J, and B6D2F1, hybrid mice which are most likely to be of interest to gerontological scientists. Where possible, the characteristics of young and old mice are included. Where no information exists about the behavior of older mice of these genotypes, but there is reason to believe that information about mice might be useful for aging research, this information is included. The purpose of the review is to acquaint gerontologists with the availability of genetically defined animal models for aging research, to present what is known about these models, and to suggest areas of research which could profit from more attention.


Treatment of hyperthyroidism with a large initial dose of sodium iodide I 131. A retrospective study was undertaken of 229 patients with hyperthyroidism due to Graves disease treated at the Cleveland Clinic with a large initial dose of sodium iodide I 131 (mean dose, 12.7 +/- 7.5 millicuries). The results indicate that a large initial dose is the most effective therapy for this disorder. Ninety percent of this group of patients required only one dose and recovered from hyperthyroidism within 12 weeks. Because we are convinced that there is no way to prevent the development of hypothyroidism in these patients, we have prescribed permanent thyroid hormone replacement after the hyperthyroidism has been controlled. In a mean five-year follow-up of 190 patients, 174 (91.5%) were still taking thyroid hormone.


The stable bubble test.

The stable bubble test.


Chronic lymphocytic leukemia and lymphosarcoma associated with multiple myeloma: report of three cases. Three patients had the rare occurrence of multiple myeloma coexisting with chronic lymphocytic leukemia or lymphosarcoma. It is not possible at present to resolve the question as to whether these two diseases represent part of the spectrum of a single B-cell disease or wether multiple myeloma and lymphoproliferative disorders are two separate entities, which may rarely occur by coincidence in the same patient.


Peridnin-chlorophyll a-proteins of dinoflagellate algae.


"...and if not, then what?".

"...and if not, then what?".


Prevention of coronary heart disease by cholesterol lowering diet.


Somatic cell hybridization: discovery and development.


Thalamo-cortical systems regulation spindle bursts and recruiting responses. III. Effects of lesions in the forebrain and rostral diencephalon.


Epileptic seizures as an early complication of neurosurgery. The appearance of epileptic seizures in the early postoperative period (first 10 days) after neurosurgery is an uncommon complication. A retrospective study of 600 patients operated on for glioma, meningioma, metastatic tumor, hydatid cyst, cerebral hemorrhage and head injury (100 cases each) is the subject of this report. In 6% of the total group, seizures were found during the first 10 days following surgery. They usually appeared during the first 48 hours. In most cases (78%), fits were of the partial motor type, originating in the frontoparietal (central) region. The clinical course was benign in most cases. Good control of seizures was usually obtained by common anticonvulsants and except for a few cases, the prognosis was not worsened by the appearance of these early fits. The pathophysiology of early seizures, which are not related to cerebral scarring, is discussed. It is concluded that they are related to biochemical alterations produced by surgery, similar to what happens in early post traumatic epilepsy or in cases of seizures appearing in the early stages of cerebrovascular accidents.


Pupillary sparing in oculomotor palsy from temporal lobe astrocytoma. Report of two cases. Two cases are reported of pupillary sparing in oculomotor palsy from temporal lobe astrocytoma. In one of them it was the first manifestation of recurrence of tumour. Tumour infiltration with resultant nerve ischemia is suggested to be the responsible pathological process.


Incompatibilities of additives to intravenous infusion fluids.


The friendly urologist: a philosophy for urology.


Specific hydrolysis of methionyl-tRNA Met f catalyzed by a purified peptide. A peptide initiation factor purified from rat liver and promoting the binding of initiator tRNA and model initiators to 40S and 80S ribosome at an acid pH liberates methionine and N-acetylmethionine from Trna Met f at neutral reaction. Phenylalanyl-tRNA, N-acetylphenylalanyl-tRNA and methionyl-tRNA Met m are not hydrolyzed under the same conditions. Hydrolysis of methionyl-tRNA Met f is stimulated by the presence of the 40S ribosomal subunit and preceeds at 37 degrees C until all the substrate has been split. No hydrolysis of initiator tRNA or N-acetylmethionyl-tRNA Met f occurs at 0 degrees C. Hydrolysis is slightly stimulated by GTP and MG2+ but not by KCl. The binding and hydrolyzing activity associated with a single protein factor may have an important function in regulating the rate of peptide initiation.


Renal cortical outline evaluation in excretory urography. Capillary phase nephrogram films and tomography were used to evaluate renal cortical outlines and renal parenchymal masses. The method of examination and the results are presented, showing the capillary phase film to be beneficial and to play a significant role in excretory urography. No increase in the incidence of side effects was noted with the rapid injection technique.


The infection control sister--aims and objectives.


Detection of renovascular hypertension: saralasin test versus renin determinations. Angiotensin blockade was established in hypertensive patients with the competitive inhibitor saralasin and the blood pressure response was compared to prior renin determinations. Two patients with subsequently confirmed renovascular hypertension had normal peripheral renin and non-lateralizing renal vein renin ratios, yet both showed a clear-cut lowering of blood pressure after administration of the blocking agent, indicating the presence of renin-mediated hypertension. Thus, direct in vivo testing with saralasin appears to offer certain advantages over renin determinations.


Gladys, in good times and bad.

Gladys, in good times and bad.


Renal vein thrombosis.

Renal vein thrombosis. The manifestations, clinical course and treatment of 14 patients with non-malignant renal vein thrombosis are described. Most patients (10 of 14) had generalized vague illness and nephrotic syndrome but 4 were initially seen with acute symptoms of flank pain, hematuria or hypertension. Renal vein thrombosis affected young men 2.5 times more often than women and occurred on the left side 2.6 times more commonly than on the right or both sides. Red blood cell casts in the urinary sediment, heavy proteinuria and hypoalbuminemia were useful indicators of the disease. Excretory urographic signs were suggestive of renal vein thrombosis in all patients and these were corroborated by angiographic studies. Systemic anticoagulation with or without a renal failure program and diuretics, or simply a combination of the last 2 modalities, was used in 9 patients. In 2 of the 9 patients who were unresponsive the adjuvant use of cyclophosphamide and steroids effected a cure. The remaining 5 patients underwent nephrectomy or thrombectomy. All 14 patients were followed for 1 to 7 years (mean 1.6 years). Ten patients were cured or improved, 1 patient was unchanged, and in the remaining 3 patients the condition deteriorated and they subsequently required a renal allograft. The rationale for various forms of treatment is discussed.


Diploma in Nursing, College of Nursing, Australia. The beginning of a new era in nursing education in Australia.


The flush versus the protruding urinary stoma. All too often arbitrary or theoretical considerations are the determinants of the external stomal dimensions at the time of ureterointestinal diversion. To the patient this facet of the operation becomes one of immediate, lasting and paramount importance. A comparative analysis of the experiences of 125 stoma patients suggests that there are distinct advantages to the protruding nipple insofar as the patient is concerned. Management and clinical problems of the flush and the protruding urinary stoma are discussed.


Selective transcatheter arterial embolization of renal carcinoma: an original technique. The association of pharmaco-angiography with norepinephrine and transcatheter arterial embolization is a technique used to obstruct arteries invaded by renal carcinoma. We treated 4 patients with this method before nephrectomy and recommend its use as a complement to an operation for renal carcinoma or as definitive treatment in poor surgical candidates.


"Brush-in" at Karuah.

"Brush-in" at Karuah.


Management of the ureterotomy incision in 100 consecutive ureterolithotomies. We reviewed retrospectively 100 consecutive ureterolithotomies in an attempt to determine if the choice of the ureterotomy closure significantly altered the postoperative course and morbidity rate. No dramatic difference was found when comparing ureterotomies left open, those closed loosely with interrupted sutures and those closed with continuous sutures. Parameters studied were the duration of postoperative urinary drainage, hospital stay and fever. Postoperative radiographic findings among the 3 groups are discussed.


Retroperitoneal iliac fossa abscess presenting as primary disease of the genitourinary tract. An iliac fossa abscess is a clinical entity that has not been described in the urologic literature. Three patients presenting with urologic symptoms were evaluated and found to have iliac fossa abscesses. It is important for the urologist to be aware of the existence, diagnosis and treatment of this entity.


Pelvic exenteration: complications of urinary diversion. We report complications directly related to urinary diversion among 32 patients who underwent pelvic exenteration for gynecologic malignancy. Eight patients (25 per cent) sustained 10 major complications, including 8 urinary fistulas and 2 fistulas from the entero-enterostomy sites. The frequency of these complications was greater among patients who had had total exenteration than among those who had had anterior exenteration. We attempt to identify factors responsible for these complications and discuss methods for reducing the morbidity related to urinary diversion in these patients.


Education for family living.

Education for family living.


Abacterial cystitis: treatment with sodium oxychlorosene.


Urethral calculi.

Urethral calculi. The diagnosis and management of 34 men and 13 women with urethral calculi are reviewed. A majority of patients presented with dysuria, weak stream and perineal pain. Diagnosis was made radiographically in only 42 per cent but was confirmed in all patients who underwent cystourethroscopy. All patients had lower urinary tract lesions or previous prostatic operations and 47 per cent were infected. Migrant urethral calculi were encountered 10 times more often than were native stones and a metabolic cause was found in 10 of 18 patients studied. In men penile urethral calculi were removed transurethrally and all bulbous and most posterior urethral stones were guided endoscopically into the bladder. Most of these stones were irrigated out or removed by transurethral litholapaxy; the remaining few, together with vesical calculi, required cystolithotomy. In all 13 women calculi were removed at the time of urethral diverticulectomy. A majority of 17 men (76.5 per cent) and the 4 women who were followed for an average of 5.3 years have remained free of trouble.


Nurse education--do we care where we are at?


The patient lives--but does the person survive?


Urethral strictures: treatment with intralesional steroids. A review of steroid management of urethral strictures is presented. The 96 patients were followed for a year or more after urethral injection with triamcinolone. Intralesional steroid may be used in many types of strictures but it is especially useful in those cases with strictures in the distal urethra or the meatus, those occurring after radical prostatectomy and in some cases when 1 or more urethroplasties have been done. Every practicing urologist can benefit from using stricture injection in selected cases.


Benign periurethral masses in women.

Benign periurethral masses in women. Benign periurethral masses were encountered in 4 women. The masses included 2 vaginal leiomyomas, 1 of which was found simultaneously with a urethral diverticulum, a fibrocystic urethral mass and hyperplastic glandular tissue obstructing the bladder outlet and resembling male prostate. The physical and endoscopic features were helpful in establishing the benign nature and extent of the lesions. Although the bladder base was deformed on an excretory urographic cystogram in each case the radiographic features were non-specific except as they applied to a urethral diverticulum. Transvaginal needle biopsy is recommended in contemplation of open surgical excision. Leiomyomas are best approached transvaginally and anterior urethral masses can be reached suprapubically.


Carcinoma of the penis.

Carcinoma of the penis. This study updates the experience at our hospital with cancer of the penis, which now includes 122 consecutive cases seen between 1940 and 1974 with 100 per cent followup. Historical, clinical, pathological, therapeutic and followup data were extracted for all cases in a digital code for transfer to computer storage. Life tables giving cumulative survival rates through 10 years with standard deviations and age corrections were calculated for each variable. The data were tabulated for publication to emphasize the prognostic significance of individual variables for the purpose of prospective research planning. The data were then evaluated regarding indications for prophylactic inguinal lymph node dissections, if any.