Effects of lidocaine and etidocaine on the axoplasmic transport of catecholamine-synthesizing enzymes.


Inhibition of rapid axoplasmic transport by procaine hydrochloride.


Removal of blood group determinants from bovine erythrocyte membranes. I. Action of proteolytic enzymes on ghosts.


Anesthetic management of the patient with hyperthyroidism.


Phenol rhizotomy in the treatment of cancer pain.


An indirect hemagglutination test for antibodies to Corynebacterium equi.


Composite complement: a mixture of feline C1 and guinea pig complement as a reagent compatible with feline antibodies.


Quantitative analysis of three classes of immunoglobulins in serum of newborn pigs and milk of sows.


Sodium and chloride transport across the equine cecal mucosa.


Sequence and rate of postmortem autolysis in guinea pig liver.


The regional difference of pleural mesothelial cells in rabbits.


The solitary pulmonary nodule--1974.

The solitary pulmonary nodule--1974.


Use of globin cDNA as a hybridization probe for globin mRNA.


Morphological and submicroscopic comparison of sickle erythrocytes of humans and deer.


The switch from fetal to adult hemoglobin in humans: evidence suggesting a role for gamma-beta gene linkage.


Embryonic and fetal hemoglobin in animals.


The K+-stimulated ATPase system of microsomal membranes from gastric oxyntic cells.


Hyaline bodies of the optic papilla and bilateral acute vascular occlusions.


Autonomic drugs in ophthalmology: Some problems and promises. Section IV: Sympatholytic and sympathetic blocking agents--alpha adrenergic antagonists and beta adrenergic antagonists.


Palliative treatment of oesophageal cancer.


Structural variation in chromosome No 9.


C bands in human metaphase chromosomes treated by barium hydroxide.


Fetuin as a trypsin inhibitor.

Fetuin as a trypsin inhibitor.


Some ultrastructural observations on keratohyalin granules of guinea pig epidermis.


The staining of free-living amoebae by indirect immunofluorescence.


Ventricular irritability associated with the use of naloxone hydrochloride. Two case reports and laboratory assessment of the effect of the drug on cardiac excitability.


Fenestrated endothelium of the liver sinusoids of the guinea pig as revealed by scanning electron microscopy.


A solicitation for referral of your patients.


A steroid-sensitive solitary intracranial plasmacytoma.


Disorders of the corneal epithelium. A clinicopathologic study of dot, geographic, and fingerprint patterns.


Preparation of undecalcified temporal bone sections.


The chemical composition of wool. XII Further studies on cortical cells and macrofibrils.


Anatomical projection of the cochlea to the cochlear nuclei of the guinea pig.


Action of some antibiotics on the electrical activity of intestinal smooth muscle of rat.


Characterization of the organophosphorus-resistant Mt Alford, Gracemere and Silkwood strains of the cattle tick, Boophilus microplus.


Contingent reinforcement of lowered blood-alcohol levels in an outpatient chronic alcoholic.


Nursing management of transurethral resection of the prostate.


Immunological unresponsiveness in mice. I. Immunological unresponsivenss induced in embryonic mice by maternofetal transfer of human gamma-globulin.


Isotope assay of histamine release.

Isotope assay of histamine release.


Localization of the XG allotypic determinants, in which carbohydrate structures take part, to the separable parts of the rabbit IgG molecule.


Activity of fosfomycin against Pasteurella.


Effect of storage on microsomal mixed function oxidase activity in mouse liver.


Standardized excision of small areas of rat and mouse brain with topographical control.


Subcellular distribution of K-strophanthoside (3H) in isolated guinea-pig hearts.


Cerebral dysfunctions--clarification, delineation, classification.


[Investigation of the local antihistamine effect of dermatological preparations].


Black grain mycetoma. A study of the chemistry, formation and significance of the tissue grain in Madurella mycetomi infection.


A computer assisted instructional course in diagnosis and treatment of the rheumatic diseases.


Elution of antibodies from the lymphocyte membrane in certain dermatoses. Preliminary communication.


Chlamydial infection of conjunctival tissues in culture.


The articular cartilage in familial chondrocalcinosis. Light and electron microscopic study.


Identification of muscle fibre types in the experimentally denervated tibialis anterior muscle of the rabbit.


Jejunal absorption rates of glucose, glycine and glycylglycine in Zambian African adults with malnutrition.


Megakaryocyte polyploidization in acute leukaemia and preleukaemia.


Immunoelectrophoretic changes in Lyell syndrome.


The cation-binding properties of gramicidin.


Coronary lipohyalinosis. Dietary induction in guinea pigs.


Focal differences in lipid metabolism of the young pig aorta. Part 2. Synthesis from (2-14c)ethanolamine and (1,2-14C)choline.


The aggregation of gramicidin A in solution.


The conformation of gramicidin A.

The conformation of gramicidin A.


A 13C nuclear magnetic resonance study of gramicidin A in monomer and dimer forms.


High-anti conformation in o-azanucleosides. The crystal and molecular structure of 6-azacytidine.


An unbiased method for estimating binding parameters in a non-cooperative binding process.


Studies on fetuin from foetal bovine serum. The composition and amino acid sequences of glycopeptides from fetuin.


Asymmetric membranes resulting from the fusion of two black lipid bilayers.


Isolation of nuclei and characterization of nuclear DNA of Acanthamoeba castellanii.


In vitro effects of silicotungstate on some RNA viruses.


The Epstein-Barr virus (EBV) in human pathology. Results of a serological investigation of a hospital population.


Antibodies to Epstein-Barr virus (EBV) antigens in sera of American Burkitt lymphoma patients.


The equivalence of fluctuation analysis and chemical relaxation measurements: a kinetic study of ion pore formation in thin lipid membranes.


The ordered structure of the encephalitogenic protein from normal human myelin.


The collection and analysis of volatile hydrocarbon air pollutants using a timed elution chromatographic technique linked to a computer controlled mass spectrometer.


Treatment of acid phosphatase deficiency disorders.


Chlorinated hydrocarbon insecticide residues in adipose, liver, and brain samples from Iowa mink.


Hexachlorobenzene (HCB) residues in fish.


Effects of DDT on bobwhite quail (Colinus virginianus) adrenal gland.


Short-term effects of organophosphate pesticides on cholinesterases of estuarine fishes and pink shrimp.


Residual organochlorine pesticides in the fat of mutton birds of New Zealand.


The susceptibility to selected insecticides and acetylcholinesterase activity in a laboratory colony of midge larvae, Chironomus tentans (diptera: chironomidae).


Lead concentrations in native trout.

Lead concentrations in native trout.


Intestinal absorption of hexachlorobenzene and hexachlorocyclohex and isomers in rats.


The pigeon, a sensor of lead pollution.

The pigeon, a sensor of lead pollution.


Simultaneous clean up of fish fat containing low levels of residues and separation of PCB from chlorinated pesticides by thin-layer chromatography.


Storage of organochlorine insecticides in adipose tissue of Ugandans.


The content of polychlorinated hydrocarbons in Arctic mammals.


Pesticide residues in the top soil of five West Alabama counties.


Effect of dihydroergotamine on partial functions of the kidneys and water and salt metabolism in dogs with experimental hypertension.


Microfluorimetric study of thermal denaturation of the nuclear DNP-complex of lymphocytes stained with acridine orange.


Detection of the mucopolysaccharide component of the supraplasmalemmal covering of cells lining the alveoli of the rat lung.


Technical difficulties in determining the origin of hair and sebaceous glands found in regenerating skin.


Analysis of changes in the nuclear chromatin of peripheral blood lymphocytes in Down's syndrome.


Double-blind cross-over trial comparing intranasal beclomethasone dipropionate and placebo in perennial rhinitis. Twenty-five patients with perennial rhinitis completed a double-blind cross-over trial of intranasal beclomethasone dipropionate 200 mug daily and placebo. Of these patients 19 preferred the active drug and two preferred placebo. There were significant reductions in symptom scores for nasal obstruction and rhinorrhoea and in the use of decongestant nasal drops when using the active drug. No changes in morning plasma cortisol levels occurred during the three-week treatment period.


An automated system for counting silver grains in autoradiographs.


The axonal transport of slowly migrating (3H)leucine labelled proteins and the regeneration rate in regenerating hypoglossal and vagus nerves of the rabbit.


Magnesium or calcium activated ATPase in mammalian nerve.


Analysis of acetylcholinesterase synthesis and transport in the rat hippocampus: recovery of acetylcholinesterase activity in the septum and hippocampus after administration of diisopropylfluorophosphate.


Suppression of the in vitro and in vivo cytotoxic effects of guanethidine in sympathetic neurons by nerve growth factor.


Characteristics of the retrograde axonal transport system for nerve growth factor in the sympathetic nervous system.


Striate cortex projections to the lateral geniculate and other thalamic nuclei: a study using degeneration and autoradiographic tracing methods in the marmoset Callithrix.


Germination studies on pure yeast ascospores.


Scanning and transmission electron microscopy of the ependymal lining of the third ventricle.


The anatomical substratum of pain evidence derived from morphometric studies on peripheral nerve.


Biology of the human malignant lymphomas. I. Establishment in continuous cell culture and heterotransplantation of diffuse histiocytic lymphomas.


Effects of bleomycin on cells in culture: a quantitative cytochemical study.


The documentation of human breast cancer in tissue culture.


Investigations into the role of pharmacologically active amines in anaphylaxis in calves.


Total knee arthroplasty.

Total knee arthroplasty.


Structural basis of atrial and ventricular conduction.


Re-entry--an important mechanism of cardiac arrhythmias.


Wenckebach type I and type II atrioventricular block (revisited).


Exercise-induced arrhythmias--recognition, classification, and clinical significance.


Pre-excitation syndrome.

Pre-excitation syndrome.


Contribution of atrial pacing to understanding of the genesis of arrhythmias.


Prognostic significance of ventricular premature beats.


The P wave: correlation of surface and intra-atrial electrograms.


Refractoriness of cardiac tissue.

Refractoriness of cardiac tissue.


Distribution of large ventral horn cells in the lumbar cord of the rat.


Identification of neurophysin producing cells. I. The origin of the neurophysin-like substance-containing nerve fibres of the external region of the median eminence of the rat.


On catecholamine-containing cells in the rat interatrial septum. Enzymatic-histochemical and elctron microscopical study.


Sensory structure of the tentacles of the slug, Arion ater (Pulmonata, Mollusca). 2. Ultrastructure of the free nerve endings in the distal epithelium.


Sensory structure of the tentacles of the slub, Arion alter (Pulmonata, Mollusca). 1. Ultrastructure of the distal epithelium, receptor cells and tentacular ganglion.


Ultrastructure of pigment cells in wild type and color mutants of the Mexican axolotl.


An ultrastructural study of the subcommissural organ cells of the African clawed toad, Xenopus laevis.


The diversity of the messenger RNA population in growing Friend cells.


The RN as a neurosurgical assistant. A new concept in total patient care.


In vitro autoradiographic studies of DNA synthesis in human testis with spermatogenic arrest.


Editorial: Trauma, splenectomy and the risk of infection.


The temperature dependence of the response to valinomycin and gramicidin by isolated liver mitochondria from warm- and cold-blooded animals.


The generation of antibody diversity: its dependence on antigenic stimulation.


Immunogenicity in vitro: structural correlation.


Radioelectrocomplexing: a general radioimmunoassay procedure for the detection of primary binding of antigen by antibody.


Static dynamic imaging of the brain.

Static dynamic imaging of the brain.


Expression of histocompatibility antigens during the growth cycle of cultured lymphoid cells.


Homogeneous antibodies: induction and value as probe for the antibody problem.


Evaluation of ligation of a branch of the portal vein for unresectable hepatic tumor.


Trypsin G- and C-banding for interchange analysis and sex identification in the chicken.


Nuclear DNA content of the emu.

Nuclear DNA content of the emu.


Recurrent seizures induced by potassium in the penicillin treated hippocampus.


Oxidative metabolism during recurrent seizures in the penicillin treated hippocampus.


Masseteric and digastric reflex activity during conditioned sensorimotor rhythm.


The maturation and interrelationship of EEF patterns and auditory evoked response in premature infants.


Huntington's chorea: an electroencephalographic and psychometric study.


Schizophrenia and EEG spectral analysis.


Focal seizures induced by movement without sensory feedback mechanisms.


Clinical electromyographic studies of diseases of the motor unit.


Transient global amnesia with a previously unreported EEG abnormality.


Differential brainstem pathways for the conduction of auditory frequency-following responses.


Early complications following operations for cancer of the rectum and anus.


Activity of muscle spindles during muscle twitch and clonus in normal and spastic human subjects.


Slow potentials of the brain related to speech processing in normal speakers and stutterers.


Remote monitoring of muscle length and EMG in unrestrained cats.


Spike-and-wave afterdischarges in cortical somatosensory neurons of cat.


A new technique for functional visual deprivation.


A universal bird head-holder for stereotaxic surgery.


Overlapping advance control systems for microelectrode positioning.


Sources of electrocochleographic responses as studied in patients with brain damage.


The importance of methodology in drug studies using EEG.


Purification and properties of Avian growth hormones.


Evidence of similar behaviour of zinc in intestinal crypts and pancreatic islets.


The influence of estrogenic and-or progestogenic treatment on some parameters of lipid metabolism (a controlled clinical study).


Alpha-fetoprotein in primary liver cancer and other diseases.


Affinity labeling and topology of the antibody combining site.


Immunologic properties of Aalpha chain of human fibrinogen.


Human leukemic cells: RNA-directed DNA polymerase.


On the use of affinity chromatography in demonstrating the transfer of thioester-bound D-phenylalanine from the light enzyme of gramicidin S synthetase to an acceptor site for this amino acid on the heavy enzyme.


Viability of tumor cells in mechanically and enzymatically prepared tissue suspensions.


Pituitary prolactin levels in canine mammary cancer.


The morphological characterization of isolated nuclei in comparison to in situ nuclei from mouse cortex.


Axonal regeneration and functional plasticity in the central nervous system.


Interaction of lindane and diazinon on the skin of rats.


The location of soluble antigen in the spleen of Xenopus laevis.


The inhibitory effect of toluidine blue and methylene blue on rat mast cell damage by promethazine, chlorpromethazine and chlorpromazine. Its reversal by glucose.


Lanthanum staining of the intermediate region of the cell wall in Escherichia coli.


Inability of specific antibodies to discriminate between frog and tadpole haemoglogins in mixture.


Electrostatic field induced changes in mouse serum proteins.


Some immunochemical studies on leukocyte antigens in acute leukemia.


Studies on phytohemagglutinins XXI. The covalent oligomers of lysozyme-First case of semisynthetic hemagglutinins.


Transformation of nucleoli in vesicular gland cells of mice.


An ammoniacal-silver stain technique specific for satellite III DNA regions on human chromosomes.


Correlation between cellular manifestations of allograft response and graft rejection times.


Haemagglutination and cytotoxic studies of H-2 IV. Evidence that there are 3-like antigenic sites determined by both the K and the D crossover regions.


Susceptibility of different avian cells to transfection with DNA isolated from virogenic mammalian Rous sarcoma cells.


Effect of host splenectomy on the course of non-H-2 allograft response in mice.


Correlative histochemical and ultrastructural studies on fetal and early postnatal guinea pig ovaries, with special reference to the development of interstitial cell system.


Experimental analysis of the migration and differentiation of neuroblasts of the autonomic nervous system and of neurectodermal mesenchymal derivatives, using a biological cell marking technique.


Acid mucopolysaccharide metabolism, the cell surface, and primary mesenchyme cell activity in the sea urchin embryo.


Muscle and cartilage differentiation in small and large explants from the chick embryo limb bud.


Cytodifferentiation of the acinar cells of the rat submandibular gland.


The inheritance of cell differentiation during limb regeneration in the axolotl.


Free amino acids in normal and tumorous tissues of human kidney, bladder, and prostate.


A barrier to diffusion in pseudoplasmodia of Dictyostelium discoideum.


Vegetative nuclear structures and their mode of division in Cyathus species.


Distribution of nuclei in macroconidia of dermatophytes.


Electron microscopic study of pyruvate dehydrogenase complex from Saccharomyces carlsbergensis.


Isolation and molecular properties of formamidase from rat liver cytoplasm.


Deficiency of lactosyl sulfatide sulfatase in metachromatic leucodystrophy (sulfatidosis).


Epithelial basement membrane of bovine renal tubuli. Isolation and chemical characterization.


Hospitals offer employees health programs.


A t(21q21q) ring chromosome.

A t(21q21q) ring chromosome.


Cytophilic antibodies in mice contact-sensitized with oxazolone. Immunochemical characterization and preferential binding to a trypsin-sensitive macrophage receptor.


Cytophilic activity of IgG2 from sera of guinea-pigs immunized with bovine gamma-globulin.


Interaction of anti-thymocyte serum with haematopoietic stem cells. II. Stimulation of colony formation in vitro.


Further evidence for the role of macrophages in the initiation of lymphocyte trapping.


Mechanism of follicular antigen trapping. Evidence for a two cell mechanism using double isotope autoradiography.


Further studies on the prevention of tolerance induction by poly A:U.


The production of precipitating antiglobulin reagents specific for the subclasses of human IgG.


Sheep antibodies to soluble rat collagen. Isolation of cross-linked peptides by affinity chromatography.


In vitro stimulation of spleen cells of the mouse by DNP--carrier complexes.


Cells involved in the in vitro stimulation by DNP-carrier complexes of in vivo primed mouse spleen cells.


Common antibody-induced redistribution on thymocytes of strain-, species- and non-species-specific antigenic determinants shared by brain and thymocytes of mice.


Stimulation of lymphocyte transformation by autologous serum fractions from patients with rheumatoid arthritis.


Cytochemical studies on the staining of DNA-aldehyde with basic dyes.


Haemagglutination inhibition assay of the common determinants and subspecificities of Australia antigen.


Alpha-fetoprotein in Indian childhood cirrhosis.


Thiacetazone concentration in blood related to grouping of tubercular patients, its treatment, results, and toxicity.


Role of macrophages in resistance to murine cytomegalovirus. The role of macrophages in protecting mice from murine cytomegalovirus (MCMV) was studied in Swiss, CBA/J, and C57BL/6J mice. CBA/J mice were more resistant to virus than were C57BL/6J mice at all ages tested. Prior treatment of adult Swiss mice with 60 mg of silica, a dose selectively toxic to macrophages, increased mortality due to MCMV infection. Transfer of syngeneic adult macrophages to suckling mice significantly increased their resistance to subsequent MCMV infection. Transfer of syngeneic, nonimmune adult lymphocytes to suckling mice also had a lesser but significant protective effect against subsequent MCMV challenge. In vitro infection of adult CBA/J and C57BL/6J macrophages with virulent and attenuated MCMV resulted in productive infection in only a small percentage of cells and recovery of very little virus from the extracellular fluid. Infection of CBA macrophages was no less productive than C57BL/6J nor was infection with virulent virus more productive than with attenuated virus. Histological examination of the livers of MCMV-infected CBA/J and C57BL/6J mice suggested that divergent cellular immune responses to infection might account for differences in susceptibility. It is postulated that the macrophage may facilitate the inductive phase of cellular immunity, one possible explanation for its demonstrated importance in host defenses against MCMV.


Influence of culture medium on the glucosyl transferase- and dextran-binding capacity of Streptococcus mutans 6715 cells. Growth of Streptococcus mutans 6715 in a medium containing trace amounts of sucrose or dextran promotes cell-associated glucosyl transferase activity and increases the dextran-binding capacity of the organisms.


Immunological studies of Brown recluse spider venom. Polyacrylamide gel electrophoresis of Loxosceles reclusa venom demonstrated that only one of seven or eight major (plus three or four minor) protein components caused necrosis in guinea pig skin. Sephadex gel filtration separated the venom into three major peaks, the second peak of which contained the dermonecrotic activity. Hyperimmunization of rabbits with increasing doses of venom from L. reclusa produced potent precipitating antisera, and the rabbits became resistant to lesion development. Ouchterlony-type immunodiffusion and immunoelectrophoretic studies revealed six to seven distinct precipitation lines, one of which stained intensely for esterase activity. Immunohistochemical techniques failed to detect any protease, lipase, catalase, acid phosphatase, alkaline phosphatase, or amylase activity in the venom. The spreading activity of recluse spider venom in guinea pig skin was inhibited as much as 71% by antivenom. Venom preincubated with antivenom was unable to incite lesions in guinea pig skin. Passive immunization of guinea pigs 18 h before an injection of venom conferred venom resistance upon the animals. Local injections of antivenom immediately after intradermal injections of venom markedly reduced the dermal lesion. Heparin reduced the local and systemic effects of venom when preincubated with whole venom or when administered systemically before an intradermal injection of venom. Treatment of whole venom with the chelating agent ethylenediaminetetraacetate did not inhibit its necrotic activity. Transfer studies from a 24-h lesion indicated that the necrotic activity was localized and remained active in tissue for at least 24 h but not for 5 days. No lesions developed when high concentrations of venom were intradermally injected into the skin of sacrificed guinea pigs, indicating that an interaction of body constituents and venom is essential for the development of a lesion.


LSD-assisted psychotherapy in patients with terminal cancer.


Malignant tumours of the bile ducts.

Malignant tumours of the bile ducts.


Human growth hormone. Characterization of plasma and pituitary components and application of a new radioreceptor assay.


Subunits of human glycoprotein hormones. Their immunological and biological behavior.


The etiology of Skid Rows in the United States.


Experimental exophthalmos. Alterations of normal hormone-receptor interactions in the pathogenesis of a disease.


The leukocyte nitroblue-tetrazolium reduction test. A rapid counting method.


Morphological changes of Mycobacterium leprae grown in mouse foot pads.


The effect of methanol on granulocyte and horseradish peroxidase quantitatively studied in a film model system.


Some histochemical reactions of mast cells of gerbils, hogs, armadillos and cats.


Ultrastructural localization of catalase on ultracryotomic sections of mouse liver by ferritin-conjugated antibody technique.


Peroxisomes in the liver of the carp (Cyprinus carpio L.). Electron microscopic cytochemical and biochemical studies.


Digestibility of elastic fibers by elastase in semifine and fine sections as a function of fixation mode and embedding media.


Formaldehyde-chloral hydrate as a fixative for mouse tissues for localisation of acid and alkaline phosphatases.


Sickle cell anemia: therapeutic considerations.


A reply to comments by Scott (Histochemistry 39, 195--196, 1974).


High-resolution scanning-densitometry of photographic negatives of human metaphase chromosomes. II. Feulgen-DNA measurements.


High-resolution scanning-densitometry of photographic negatives of human metaphase chromosomes. I. Instrumentation.


Breakage of DNA-membrane complex by bleomycin.


Study on alpha-1 antitrypsin enzyme deficiency in adult cirrhosis. A preliminary communication.


Curing action of sodium dodecyl sulfate on a Proteus mirabilis R+ strain. Growth of Proteus mirabilis harboring R100-1 (fi(+)drd str(r)cml(r)tet(r)sul(r)) factors in Penassay broth containing sodium dodecyl sulfate (SDS) leads to the loss of all or part of the genetic elements in high frequencies. In media containing SDS at concentrations as low as 0.03%, both lysis of R(+) cells and elimination of the R factors occur at high frequencies. Appearance of drug-susceptible cells in R(+) cultures occurs during the exponential phase of growth; however, the frequencies of susceptible cells increase substantially after the culture reaches the stationary phase. Reconstruction experiments, coupled with other observations, suggest that the major factor in altering the frequency of drug-susceptible variants is the greater resistance of the variants to the lytic action of SDS. This resistance correlates in most cases with the loss of the transfer functions in the resistance transfer factor.


Bovine plasma kininogens. III. Structural comparison of high molecular weight and low molecular weight kininogens.


Two-dimensional arrays and particles in negative staining preparations of fragmented human erythrocyte ghosts.


Mechanism of action of ribonucleic acid-directed deoxyribonucleic acid polymerase. I. Transcription of globin messenger ribonucleic acid.


A five-channel multimedia programmer constructed from surplus parts.


The role of carbohydrate in platelet adhesion to foreign surfaces.


The high voltage electron microscope in biology.


Effects of calcium-containing fixation solutions on cholinergic synaptic vesicles. Calcium (Ca)-containing fixation solutions applied to slices of electric organ of the electric ray, Narcine brasiliensis, have been shown to have three distinct ultrastructural effects on cholinergic synaptic vesicles of the nerve terminals. (a) An electron-dense particle (EDS) is observed within the vesicle; the particle is seen in unosmicated, unstained tissues and can be removed from thin sections by Ca-chelating agents. It is concluded that the EDS represents Ca bound by the vesicle. It is suggested that the bound ATP of the vesicle provides anionic Ca binding sites. (b) The vesicle membrane tends to 'crinkle' or collapse depending on the concentration of the other components of the fixative solution. The 'crinkling' or collapse are largely reversed by a wash step in the absence of Ca. (c) The presence of Ca results in the appearance of a population of vesicles which form characteristic fusions or 'tight' junctions with the terminal membrane. This appears to be morphological evidence for the proposal, which has been frequently put forward, that Ca facilitates such a fusion before discharge of vesicle-bound transmitter. With the discovery that the use of Ca-containing fixatives leads to the demonstration of a subpopulation of synaptic vesicles fused to the terminal membrane, we are led to propose that this is the ultrastructural location of the newly synthesized acetylcholine which has been shown by others to be preferentially released by stimulation.


Change of chromatin morphology during the cell cycle detected by means of automated image analysis.


Blood viscosity in patients with peripheral vascular diseases in the area of low shear rates.


The visualization of the photosynthetic coupling factor in embedded spinach chloroplasts. Spinach chloroplast lamellae were stained with aqueous uranyl acetate immediately after glutaraldehyde-osmium fixation but before dehydration and embedding. Under these conditions, the lamellae are shown in thin sections to have 95-A x 115-A coupling factor particles on their surfaces. The particles can be seen only on the matrix side of nonopposed thylakoids, and are shown to occur on both stromal and granal lamellae, regardless of the organization of the lamellae into stacks. It is estimated that, in native, fully coupled chloroplast lamellae, there is on the average one coupling factor for every 500 chlorophyll molecules. The morphological appearance of the particles is not affected by a variety of buffers, by changes in illumination or temperature, or by alterations in the energy state of the membranes during preparation. The particles can be removed from the membranes with low concentrations of Na(2)EDTA, and the photophosphorylating activity of the membranes is concomitantly lost. Both the activity and the appearance of the particles can be restored to the membranes by rebinding EDTA-extracted coupling factors to the uncoupled membranes.


Pinocytosis in fibroblasts. Quantitative studies in vitro. Horseradish peroxidase (HRP) was used as a marker to determine the rate of ongoing pinocytosis in several fibroblast cell lines. The enzyme was interiorized in the fluid phase without evidence of adsorption to the cell surface. Cytochemical reaction product was not found on the cell surface and was visualized only within intracellular vesicles and granules. Uptake was directly proportional to the administered concentration of HRP and to the duration of exposure. The rate of HRP uptake was 0.0032-0.0035% of the administered load per 10(6) cells per hour for all cells studied with one exception: L cells, after reaching confluence, progressively increased their pinocytic activity two- to fourfold. After uptake of HRP, L cells inactivated HRP with a half-life of 6-8 h. Certain metabolic requirements of pinocytosis were then studied in detail in L cells. Raising the environmental temperature increased pinocytosis over a range of 2-38 degrees C. The Q(10) was 2.7 and the activation energy, 17.6 kcal/mol. Studies on the levels of cellular ATP in the presence of various metabolic inhibitors (fluoride, 2-desoxyglycose, azide, and cyanide) showed that L cells synthesized ATP by both glycolytic and respiratory pathways. A combination of a glycolytic and a respiratory inhibitor was needed to depress cellular ATP levels as well as pinocytic activity to 10-20% of control values, whereas drugs administered individually had only partial effects. In spite of the availability of an accurate quantitative assay for fluid and solute uptake, the function of pinocytosis in tissue culture cells remains unknown.


Specificity of heteroantisera to human acute leukemia-associated antigens. Antisera have been raised to human leukemic blast cells from individual patients in mice rendered tolerant with cyclophosphamide to remission leukocytes from the same individual. 10 antisera were raised against acute myelogenous leukemia (AML) cells and 5 antisera were raised against acute lymphoblastic leukemia (ALL) cells. Antisera to AML cells were absorbed with ALL cells, and antisera to ALL cells were absorbed with AML cells. Unabsorbed and absorbed antisera as well as antisera raised in nontolerant mice were tested for cytotoxicity against various cells of a panel containing myeloblasts from 35 patients with AML, lymphoblasts from 7 patients with ALL, myeloblasts from 7 patients with chronic myelogenous leukemia (CML) in blast crisis, peripheral blood leukocytes from 12 patients with acute leukemia in remission and 30 nonleukemic patients, and nucleated bone marrow cells from 10 nonleukemic patients. Unabsorbed antisera to AML or ALL cells raised in tolerant mice were highly cytotoxic to leukemic blasts cells but significantly less cytotoxic to remission and control cells. Antisera to AML cells absorbed with ALL cells retained measurable cytotoxicity against AML cells but were not cytotoxic to ALL cells or control cells. Similarly, antisera to ALL cells absorbed with AML cells retained significant cytotoxicity only to ALL cells. Control antisera raised in nontolerant mice were cytotoxic to all cells tested. Although species specific, histocompatibility, differentiation, maturation, and cell cycle-associated antigens may be responsible in part for the cytotoxic activity of the unabsorbed antisera, the absorbed antisera are probably detecting antigens specific for their leukemic cell type.


The "no-reflow" phenomenon after temporary coronary occlusion in the dog. The role of microvascular damage in the genesis of the "no-reflow" phenomenon was investigated in the left ventricular myocardium of dogs subjected to temporary occlusions of a major coronary artery for 40 and 90 min. Intravenous carbon black or thioflavin S (a fluorescent vital stain for endothelium) were used to demonstrate the distribution of coronary arterial flow in control and damaged myocardium. These tracers were injected simultaneously with release of the coronary occlusion or after 5 or 20 min of reflow of coronary arterial blood. After 40 min of ischemia plus arterial reperfusion, usually the tracers were evenly distributed throughout the damaged tissue at each time of reperfusion. On the other hand, when reflow was allowed after 90 min of ischemia, portions of the inner half of damaged myocardium were not penetrated by the tracers. Electron microscopic study of this poorly perfused tissue revealed severe capillary damage; endothelial cells with large intraluminal protrusions and decreased pinocytic vesicles were common. Also, occasional intraluminal fibrin thrombi were noted, as well as extravascular fibrin deposits and erythrocytes. Myocardial cells were swollen in both poorly perfused and well-perfused irreversibly injured tissue. Contraction bands and mitochondrial Ca(2+) accumulation were prominent features of irreversible injury with reflow at 40 min but were not noted after 90 min of ischemia in areas with poor perfusion. These results suggest that 40 min of ischemia were tolerated by the capillary bed of the dog heart without serious capillary damage or perfusion defects, but that 90 min of ischemic injury was associated with the "no-reflow" phenomenon, i.e., failure to achieve uniform reperfusion. This failure of reflow was associated with extensive capillary damage and myocardial cell swelling. Death of severely ischemic myocardial cells in this model occurs before the onset of capillary damage and the no-reflow phenomenon.


Immunochemical studies of plasma kallikrein. A monospecific antibody against human plasma kallikrein has been prepared in rabbits with kallikrein further purified to remove gamma globulins. The antisera produced contained antikallikrein and also anti-IgG, in spite of only 8% contamination of kallikrein preparation with IgG. The latter antibody was removed by adsorption of antisera with either Fletcher factor-deficient plasma or with purified IgG. Both kallikrein and prekallikrein (in plasma) cross-react with the antibody with no apparent difference between the precipitation arcs developed during immunoelectrophoresis and no significant difference in reactivity when quantified by radial immunodiffusion. Kallikrein antibody partially inhibits the esterolytic and fully inhibits the proteolytic activity of kallikrein. In addition, the antibody inhibits the activation of prekallikrein, as measured by esterase or kinin release. The magnitude of the inhibition is related to the molecular weight of the activator used. Thus, for the four activators tested, the greatest inhibition is observed with kaolin and factor XII(A), while large activator and the low molecular weight prekallikrein activators are less inhibited. With the kallikrein antibody, the incubation of kallikrein with either plasma or partially purified C1 esterase inactivator results in a new precipitin arc, as detected by immunoelectrophoresis. This finding provides physical evidence for the interaction of the enzyme and inhibitor. No new arc could be demonstrated between kallikrein and alpha(2)-macroglobulin, or alpha(1)-antitrypsin, although the concentration of free kallikrein antigen decreases after interaction with the former inhibitor. By radial immunodiffusion, plasma from healthy individuals contained 103+/-13 mug/ml prekallikrein antigen. Although in mild liver disease, functional and immunologic kallikrein are proportionally depressed, the levels of prekallikrein antigen in plasma samples from patients with severe liver disease remains 40% of normal, while the functional kallikrein activity was about 8%. These observations suggest that the livers of these patients have synthesized a proenzyme that cannot be converted to active kallikrein.


Practice related exercises--an approach to teaching preclinical technique.


Anatomical analysis of pupillary reflex pathways in the rhesus monkey.


Letter: Further risk with bleomycin.

Letter: Further risk with bleomycin.


Solid tumor chemotherapy: new drugs and horizons.


ACtivity stain for the detection of L-asparaginase- anti-L-asparaginase complexes in agar.


The simultaneous detection of two protein antigens in lymphoid tissues by combining immunofluorescence and autoradiography.


Morphologic, biologic, and cytochemical analysis of intracytoplasmic particles found in cultured fibroblasts from patients with systemic lupus erythematosus.


The simultaneous detection of 3H- and 14C-labelled cells by double-layer autoradiography.


Phenformin therapy of chemical diabetes.


Cardiac surgery in early infancy, updated.


Localization of rhodomelanochrome in melanosomes of leaf frogs.


Revertants of mouse cells transformed by murine sarcoma virus: flat variants without a rescuable sarcoma virus from a clone of BALB/3T3 transformed by Kirsten MSV.


14 and 6 Per second positive spiking. Developmental and clinical correlates in psychiatric patients and their siblings.


Axonal transport and turnover of proline- and leucine-labeled protein in the goldfish visual system.


The P2 protein of bovine root myelin: isolation and some clinical and immunological properties.


Studies of axon permeability utilizing axoplasm extrusion techniques with single isolated axons from the circumesophageal connectives of Homarus americanus.


The extent of axoplasmic transport during development, determined by migration of various radioactively-labelled materials.


Proteolytic enzymes and experimental demyelination in the rat and monkey.


The isolation and properties of large basic peptides from bovine spinal cord.


Adrenaline and noradrenaline cells in the adrenal medulla of the hamster: a morphological study of their innervation.


The fine structural differences in human muscle fiber types based on peroxidatic activity.


The zinc-iodide-osmium reactive sites in the sensory epithelia of the frog labyrinth.


External version in the rhesus negative woman.


Maternal serum sialomucins during and after normal pregnancy.


Antibodies against endocardium, vascular structures, and interstitium of striated muscle that cross-react with T. cruzi and T. rhodesiense.


Iron gallein as a substitute for iron hematoxylin in parasitological staining.


Metabolic and ionic requirements for the axoplasmic transport of dopamine beta-hydroxylase. 1. Metabolic and ionic requirements for the intra-axonal transport of dopamine beta-hydroxylase (DBH) were investigated in the cat hypogastric nerve-inferior mesenteric ganglion preparation in vitro by monitoring the enzyme accumulation above a crush, 2-2.5 cm distal to the ganglion.2. DBH accumulation in the proximal segment immediately above the crush increased linearly up to 6 h, during incubation in normal Krebs solution at 37 degrees C. The rate of transport of the enzyme was about 4 mm/h.3. Removal of the ganglion, electrical stimulation or reserpine pretreatment (1-6 days before the experiment) did not modify the rate of DBH accumulation.4. Anoxia and glucose deprivation, singly, did not affect accumulation of DBH; however, the combined treatment of anoxia plus glucose deprivation, or dinitrophenol plus glucose deprivation, very markedly interfered with accumulation.5. Removal of sodium or potassium from Krebs solution markedly inhibited the transport of DBH. Preincubation of the nerve in a high-calcium Krebs solution at 4 degrees C, and then reincubation at 37 degrees C, prevented the enzyme accumulation.6. N-ethylmaleimide, ouabain and oligomycin markedly inhibited the transport of DBH.7. The results suggest that transport of DBH, and therefore of noradrenaline storage vesicles, within the hypogastic nerve is dependent on metabolic energy derived from either glycolysis or oxidative phosphorylation. It is also suggested that the sodium-potassium-activated ATPase may play an important role in the intra-axonal transport of storage vesicles.


The movement of optically detectable organelles in myelinated axons of Xenopus laevis. 1. Intra-axonal organelles were detected by darkfield and Nomarski microscopy in isolated myelinated nerve fibres from Xenopus laevis. Nerve fibres from the 8th spinal roots, the sciatic nerve, and identified motor and sensory axons from other hind limb nerves were used. The movement of the organelles was recorded either on motion picture film or by noting the times at which they crossed the lines of an ocular grid.2. Three groups of organelles were detected in all fibres. A group of particles with round profiles 0.2-0.5 mum in diameter moved somatopetally. Another group of round particles moved somatofugally. The ratio of the number of somatopetally travelling particles to the number of somatofugally travelling particles was about 10:1. The third group of organelles consisted of rod-shaped bodies about 0.2-0.3 mum in diameter and 1-8 mum in length; these were usually stationary.3. All the round particles appeared to move independently of each other with a saltatory motion. The somatopetally and somatofugally travelling particles had statistically different mean velocities of 0.98 and 1.32 mum/sec respectively.4. Round particles often crossed the node of Ranvier with no appreciable change in velocity. Some, however, were temporarily arrested at the entrance to the node.5. While the rod-shaped organelles were usually stationary, they occasionally moved rapidly lengthwise for distances of up to 10 mum. Rarely a rod-shaped organelle exhibited a continuous saltatory motion.6. Round particles often travelled in either direction along the edge of rod-shaped organelles. One rod was observed to move along the path previously taken by a round particle.7. The findings are discussed with respect to (a) the normality of the preparations, (b) the numbers of particles travelling in each direction, (c) the nature of the organelles, and (d) the mechanisms underlying the motion.8. We suggest that particles move along microtubules which have specific directionalities and particle affinities. The microtubules are in bundles and are closely associated with rod-shaped mitochondria.


The diagnostic value of detecting eosinophilia of nasal secretions in allergic rhinitis. Nasal mucus eosinophilia was found in 92 per cent of adults with active hay fever. After an initial period of gaining experience in the method of collecting mucus, this was found to be a simple cheap method of demonstating an allergic component in the genesis of rhinitis. This test could be employed for detecting the relationship of allergic factors to chronic respiratory tract disease in childhood and in adults.


Interrupted aortic arch and ventricular septal defect: palliation in a neonate.


Studies on specificity of cell-mediated immunity to human tumors.


Effect of antigen density on complement-mediated lysis, T-cell-mediated killing, and antigenic modulation.


Effect of immunoregulatory alpha-globulin on in vitro proliferative response of immune rat lymphocytes to syngeneic Gross virus-induced lymphoma.


Recovery of murine xenotropic type-C virus from C57L mice.


The nephrotic syndrome of childhood in Thailand. Histological, immunohistological and clinical correlations.


Advanced carcinoma of bladder: treatment using hypogastric artery infusion with 5-fluorouracil, either as a single agent or in combination with bleomycin or adriamycin and supervoltage radiation.


Fibroadenoma of the urachus.

Fibroadenoma of the urachus.


Study on tuberactinomycin-N. I. In vitro Antibacterial activity of TUM-N on Tubercle bacilli isolated from patients.


The incidence of myocardial abnormalities in man related to the level of ethanol consumption. A proposal of a diagnostic criterion of alcoholic cardiomyopathy.


Persistent Masugi nephritis induced in rats in spite of the suppression of host antibody responses against the heterologous nephrotoxic antibodies.


Tolerance induction in mice by continuous infusion of antigens.


The management of extrahepatic bile duct carcinoma.


Alpha-fetoprotein in the treatment of primary liver cancer.


Changes in liver function after hepatic dearterialization in man.


Dietary cadmium and enteropathy in the Japanese quail: histochemical and ultrastructural studies.


Natural history of phenylketonuria and influence of early treatment. This study describes the intellectual development of twenty-eight sibling pairs with phenylketonuria in which the index case presented because of retarded development. In twelve pairs (group I) the "second" sibling was born subsequently, and in sixteen (group II) there was a second sibling already in the family at the time of diagnosis of the index case. The intellectual outcome of the second siblings of group II suggests that approximately 1 in 6 or 7 patients with untreated classical phenylketonuria achieves an I.Q. above 70, although with evidence of intellectual impairment. The highly significant difference in intellectual outcome between the early treated second siblings of group I and the late or untreated second siblings of group II demonstrates the beneficial effect of early treatment on the intelligence of affected children.


Letter: Gammopathy and carcinoma.

Letter: Gammopathy and carcinoma.


Detection of recurrence of large-bowel carcinoma by radioimmunoassay of circulating carcinoembryonic antigen (C.E.A.). The usefulness and limitations of the carcinoembryonic antigen (C.E.A.) radioimmunoassay for the evaluation of tumour resection and for the detection of tumour relapse were studied in patients with large-bowel carcinoma. The level of plasma-C.E.A. was determined before any treatment in a group of 101 patients with histologically proven adenocarcinoma of the colon and rectum. 71% of all patients and 63% of cases with localised tumour (Dukes A and B) had a preoperative C.E.A. value of 5 ng. per ml. or higher. This limit was reached by only 1 of 90 apparently healthy, non-smoking blood-donors. Among 45 patients for whom a complete tumour resection was reported, all patients except 5 showed a drop of C.E.A. to normal values after surgery. The 5 patients whose C.E.A. did not fall to below 5 ng. per ml. showed a subsequent rise in C.E.A. level and were all found later to have a tumour relapse. The results indicate that an incomplete drop of circulating C.E.A. level one month after surgery has a bad prognostic significance. 22 of these patients were followed up by repeated C.E.A. radioimmunoassay for several months after surgery. 8 showed a progressive increase in C.E.A. levels preceding clinical diagnosis of tumour relapse by two to ten months. 6 other patients showed a moderate increase in C.E.A. levels, suggesting a tumour relapse not yet clinically detectable. The remaining 8 patients showed no increase in C.E.A. level above 5 ng. per ml. and no clinical symptoms of relapse. The results demonstrate that relapses of colon and rectum carcinoma can be detected by increased C.E.A. levels months before the appearance of any clinical evidence.


Ascorbic acid supplementation in the treatment of pressure-sores. In a prospective double-blind controlled trial the effect of large doses of ascorbic acid on the healing of pressure-sores has been assessed. 20 surgical patients were studied, the pressure areas being assessed by serial photography and ulcer tracings. The mean ascorbic-acid levels in treated and non-treated groups one month after the start of treatment were 65.6 and 25.8 mug per 10-8 white blood-cells. In the group treated with ascorbic acid there was a mean reduction in pressure-sore area of 84% after one month compared with 42.7% in the placebo group. These findings are statistically significant (P less than 0.005) and suggest that ascorbic acid may accelerate the healing of pressure-sores.


The viability of parenchymal liver cells isolated from young and old rats.


Vagal control of glucagon release in man. A significant fall in fasting plasmapancreatic-glucagon was produced by injection of atropine. After arginine infusion atropine reduced the normal rise of glucagon by 33% and glucose by 40%. Eleven patients studied during insulin hypoglycaemia after a selective vagotomy showed a peak rise in plasma-glucagon of 173 pg. per ml., which was not significantly different from the glucagon rise in ten preoperative controls (168 pg. per ml.). The glucagon response to insulin hypoglycaemia (81 pg. per ml.) in a matched group of ten patients with truncal vagotomy was significantly less than after selective vagotomy. This evidence of parasympathetic control of the alpha cell accords with morphological and laboratory-animal findings. Maintenance of normal glucagon release after selective vagotomy favours the use of this operation rather than the cruder truncal vagotomy.


Changes in cortisol metabolism following rifampicin therapy. A patient with Addison's disease required increased corticosteroid dosage whilst receiving rifampicin. The pharmacological half-life of cortisol was reduced, but returned to normal when rifampicin was stopped. Cortisolproduction rates in four patients with pulmonary tuberculosis rose during treatment with rifampicin, as did urinary D-glucaric-acid excretion, an index of liver microsomal-enzyme activity. The alteration of the corticosteroid requirement in the patient with Addison's disease and the elevation of the cortisol-production rates were attributed to increased cortisol catabolism following hepatic macrosomal-enzyme induction by rifampicin.


Effect of glycerophosphate on lithogenic bile. A new approach to treatment of cholelithiasis. Previous studies in animals showed a twofold increase of bile-phospholipids when glycerophosphate, a precursor of phospholipids, was incorporated in chow diet. The present study evaluates the effect of short-term administration of beta-glycerophosphate on bile composition in patients with lithogenic bile. 10 patients with lithogenic bile were selected for study; 9 had cholelithiasis. Fasting hepatic and gallbladder bile before and after treatment with beta-glycerophosphate (6 g. daily for six days) was analysed for molar ratios of cholesterol (Ch.), total bile-salts (B.S.), and total phospholipids (P.L.). The molar ratios of Ch. divided by B.S. + P.L. in hepatic bile decreased in 9 of the 10 patients (P less than 0.01). Thus in all but 1 of the 10 patients the hepatic bile became less lithogenic. This observation was confirmed by plotting the molar percentages on triangular coordinates. The results suggest no change in cholesterol concentrations but an increase of phospholipid and bile-salt concentrations. The composition of the gallbladder bile did not change. These preliminary studies indicate that beta-glycerophosphate should be evaluated as a potential new therapeutic agent for dissolution of cholesterol gallstones in man.


Radioimmunoassay of cannabinoids in blood and urine. A radioimmunoassay (R.I.A.), using a sheep antiserum and 3-H-T.H.C., has been developed for the measurement of tetrahydrocannabinol (T.H.C.) in plasma and urine. The antiserum cross-reacts with T.H.C. metabolites, but nevertheless possesses sufficient group specificity and sensitivity to permit measurement of cannabinoids in plasma and urine of animals and volunteers following the administration of pure T.H.C. Subjective effects experienced by the volunteers seemed to correlate with plasma-T.H.C. levels. The examination of random plasma urine samples collected from patients attending a drug-addiction clinic revealed the presence of measurable amounts of cannabinoids in several of them.


The effects of dimethylaminoethyl p-chlorophenoxyacetate on spinal ganglia neurons and satellite cells in culture. Mitochondrial changes in the aging neurons. An electron microscope study.


Simplified method for diagnosis of genital and ocular infections with Chlamydia. The diagnosis of infections with chlamydia related to the trachoma agent is best confirmed by isolation of the organism in tissue-culture. A simplified technique using conventional monolayers of McCoy cells can be used as an alternative to current methods involving heavily irradiated cells and appears to be of reasonable sensitivity. Chlamydial infection was confirmed in 38/190 females attending venereal clinics, in 22/35 patients with hypertrophic cervical erosions, and in 7/18 infants with ophthalmia neonatorum.


Pathogenesis of lipoid nephrosis: a disorder of T-cell function. Clinical observations suggest that lipoid nephrosis is produced by a systemic abnormality of T-cell function resulting in the secretion of a circulating chemical mediator toxic to an immunologically innocent glomerular basement membrane. The lack of evidence of a humoral antibody response, remission induced by measles which modifies cell-mediated immunity, the therapeutic benefits of steroids and cyclophosphamide which also abate cell-mediated responses, and the occurrence of this syndrome in Hodgkin's disease support this hypothesis. The susceptibility of untreated patients to pneumococcal infections may be of primary or secondary pathogenetic importance. Taken together, the data suggest that this syndrome is a clinical expression of a self-limited primary immune-deficiency disease.


Editorial: Confinement of the poxvirus.

Editorial: Confinement of the poxvirus.


Editorial: Long-term intravenous feeding.


Editorial: Electrical stimulation of the brain.


Editorial: Thallium.

Editorial: Thallium.


Editorial: Grey-scale ultrasonography.

Editorial: Grey-scale ultrasonography.


Training of medical teachers.

Training of medical teachers. The need for teacher training in the health professions and the shortcomings of the usual methods of involving teachers in teaching strategies are discussed. An outline of a course employing a workshop method  to overcome some of the shortcomings is given, based on the four years' experience of the course held at King's College Hospital Medical School.


Training teachers of general practice.

Training teachers of general practice.


Teacher training for medical schools in Africa. Though the problem areas of medical education in Africa have been defined and steps have been taken to attack them, notably by preparing teaching staff as agents of change and by opening teacher-training centres, progress cannot be otherwise than slow.


Educational assistants.

Educational assistants. Certain needs of medical education might be met through a team approach by which clinical teachers, in particular, can save time and effort in the planning, implementation, and assessment of education. This could be achieved through the help of educational consultants whose relationship to the teachers, as well as their responsibilities and training, are here discussed.


Environmental stress and thyrotoxicosis. Absence of association. The incidence of treated thyrotoxicosis in Northern Ireland has been estimated by the technique of "therapeutic auditing" for the periods (a) three years preceeding the start of the present civil unrest and (b) the ensuing three years. No change in incidence was found, which supports the concept that environmental stress is not important in the pathogenesis of this disease.


Pseudomonas aeruginosa in hospital sinks. Pseudomonas aeruginosa was isolated from sink waste-traps in 27 of 116 (23.3%) samples from a large general hospital and from 19 of 47 (40.4%) samples from a burns unit at another hospital. Smaller proportions of samples from sink outlets and surfaces of basins yielded Ps. aeruginosa. A waste-trap heater ('Econa') used twice daily for fifteen minutes reduced the isolations of Ps. oeruginosa from waste-traps to a very low level; isolations of other organisms were also reduced, though to a smaller extent. Despite the continuing high frequency of Ps. aeruginosa in sinks and some other moist hospital sites, Ps. aeruginosa infections were infrequent in the general hospital and had been greatly reduced by the successful use of various prophylactic measures in the burns unit.


Letter: Blood fractions for haemophilia.


Letter: Medical nemesis.

Letter: Medical nemesis.


Letter: "Wild white" viruses and smallpox.


Letter: Evaluation of laboratory testing.


Letter: Classification of non-Hodgkin's lymphomas.


Letter: Origin of malignant lymphomas.

Letter: Origin of malignant lymphomas.


Letter: Grey-scale ultrasonography in the investigation of obstructive jaundice.


Letter: Manipulating the patient.

Letter: Manipulating the patient.


Letter: Hyposplenism and ulcerative colitis.


Letter: Myopathies: the neurogenic hypothesis.


Letter: Carcinogenic potential of magnesium deficiency.


Letter: Peak-flow meter v. peak-flow gauge.


Letter: Cadaver-kidney donation.

Letter: Cadaver-kidney donation.


Letter: Treatment of anorexia nervosa by levodopa.


Letter: Proportional mortality among vinyl-chloride workers.


Letter: Trauma and cancer.

Letter: Trauma and cancer.


Letter: "Localised hypertrophic neuropathy" and hypertrophic polyneuropathy.


Letter: Sterile operating-theatres.

Letter: Sterile operating-theatres.


Letter: Reye's syndrome or not?

Letter: Reye's syndrome or not?


Letter: Fenfluramine and dreaming.

Letter: Fenfluramine and dreaming.


Letter: Weather and myasthenic fatigue.

Letter: Weather and myasthenic fatigue.


Letter: Lithium, Ebstein's anomaly, and other congenital heart defects.


Letter: Transplacental lithium poisoning.


Letter: Protein requirement.

Letter: Protein requirement.


Letter: Autoinhibition of P.H.A. response by chronic-lymphocytic-leukaemia serum.


Letter: Infantile cataract and galactose metabolism.


Letter: Methylprostaglandin e2 methyl ester and gastric-ulcer healing.


Letter: Testosterone cream: use or abuse?


Letter: Microbial-antigen detection.

Letter: Microbial-antigen detection.


Letter: Nature of nuclear projections in adenocarcinoma of the breast.


Letter: Diagnosis of insulinomas by suppression tests.


Letter: Storage and transport of serum for prolactin radioimmunoassay.


Letter: Anti-epileptic drugs and failure of oral contraceptives.


Letter: Labelled leucocytes in thrombi.

Letter: Labelled leucocytes in thrombi.


Letter: Oxytocin and neonatal jaundice.

Letter: Oxytocin and neonatal jaundice.


Letter: Iron and resistance to infection.


Letter: Dermatoglyphic nomogram in diagnosis of trisomy 21.


Letter: Paramyxovirus-like structures in Goodpasture's syndrome.


Robert Cruickshank.

Robert Cruickshank.


Edward Falkner Hill.

Edward Falkner Hill.


John Nathaniel Cumings.

John Nathaniel Cumings.


William Johnston.

William Johnston.


Chemoimmunotherapy of adult acute leukaemia. Prolongation of remission in myeloblastic leukaemia with B.C.G.


Prednisone in Duchenne muscular dystrophy.


Paget's bone disease: response to human calcitonin in patients resistant to salmon calcitonin.


Impairment of folate absorption by systemic bacterial infections.


Hypertonicity of the cricopharyngeal sphincter: A cause of globus sensation.


Increased plasma levels of 15-keto-13,14-dihydro-prostaglandin F2X after allergen-provoked asthma in man.


Availability of three antibiotics after intramuscular injection into thigh and buttock.


Autoimmune mechanisms in human vascular disease.


Editorial: Anaemia during pregnancy.

Editorial: Anaemia during pregnancy.


Editorial: Carry on talking.

Editorial: Carry on talking.


Editorial: Predisposition to thrombosis.


Editorial: Chemoprophylaxis of meningococcal infections.


Editorial: Burn toxin.

Editorial: Burn toxin.


Editorial: Acute arsine poisoning.

Editorial: Acute arsine poisoning.


Editorial: Students as patients.

Editorial: Students as patients.


Multiphasic screening.

Multiphasic screening.


Teaching geriatric medicine.

Teaching geriatric medicine.


Letter: Diet and geographical distribution of multiple sclerosis.


Letter: Aetiopathogenesis of multiple sclerosis.


Biochemical profiles for hospital patients.


Biochemistry of well populations.

Biochemistry of well populations.


Letter: Enzyme antidote for experimental cyanide poisoning.


Letter: The consultants' contract.

Letter: The consultants' contract.


Letter: Doctors and social workers.,.

Letter: Doctors and social workers.,.


Letter: Sanctions and the National Health Service.


Letter: The nurse in the community.

Letter: The nurse in the community.


Letter: Detection of breast cancer.

Letter: Detection of breast cancer.


Letter: Screening for spina bifida.

Letter: Screening for spina bifida.


Letter: Antibodies against thyroid cells in Yersinia enterocolitica infection.


Letter: Screening for cardiovascular diseases.


Letter: Monoamine-oxidase and migraine.

Letter: Monoamine-oxidase and migraine.


Letter: Mortality and natural fluoride content of drinking-water.


Letter: Effects of hysterectomy.

Letter: Effects of hysterectomy.


Letter: Malignant hyperthermia: a human stress syndrome?


Letter: Inadequate mothers.

Letter: Inadequate mothers.


Letter: Artificial kidney and artificial liver.


Letter: Blood fractions for haemophilia.


Letter: Localisation of phaeochromocytoma.


Letter: Recovery after minor head injury.


Letter: Fetal lung maturation in toxaemia and oestrogen therapy in management of respiratory distress syndrome.


Letter: Follow-up in tuberculosis.

Letter: Follow-up in tuberculosis.


Letter: Myelopathy in cervical spondylosis.


Letter: Divalent cations in predicting antidepressant response to lithium.


Letter: Influence of age on neurohormonal receptors.


Letter: A time to be born.

Letter: A time to be born.


Letter: Time dimension of the gene.

Letter: Time dimension of the gene.


Letter: Potassium supplements.

Letter: Potassium supplements.


Letter: Health of plutonium workers.

Letter: Health of plutonium workers.


Letter: Monoamniotic twins.

Letter: Monoamniotic twins.


Letter: A simple non-invasive technique for measuring cardiac contractility.


Letter: Diagnostic accuracy of vaginal smears.


Letter: Death-rates from diabetes.

Letter: Death-rates from diabetes.


Letter: Doctors and smoking.

Letter: Doctors and smoking.


Letter: Duplication of 21 or 8-21 translocation in acute leukaemia.


Letter: Plasmapheresis in rhesus isoimmunisation.


Letter: Lithium and M.R.I.H. in tardive dyskinesia.


Letter: Treatment of malignant melanoma.


Letter: Fetal adiposity in infants of diabetic mothers.


Letter: Plasma-diazepam levels following intramuscular injection by nurses and doctors.


Letter: Treatment of enuresis.

Letter: Treatment of enuresis.


Letter: Thrombocytopenia after intralipid infusion in a neonate.


Letter: Familial Hodgkin's disease.

Letter: Familial Hodgkin's disease.


Letter: Serum-gentamicin.

Letter: Serum-gentamicin.


Obituary: Douglas Cameron Lavender.

Obituary: Douglas Cameron Lavender.


Radionuclide brain scanning in investigation of late-onset seizures.


Therapeutic effectiveness of oral testosterone.


Mortality in adults and trace elements in water.


Sclerosing peritonitis, an unusual reaction to a beta-adrenergic-blocking drug (practolol).


Mortality from malignant disease in patients with asthma.


In-vitro interactions between antitumour antibodies and anti-antibodies in malignancy.


Transfusion of plasma-protein fraction and renal-allograft rejection.


Distribution of cerebral activity in chronic schizophrenia.


A device to prevent disconnection at 3-way tap junctions.


Editorial: Are sex hormones teratogenic?


Editorial: Treatment of acromegaly.

Editorial: Treatment of acromegaly.


Editorial: The giraffe's parameter.

Editorial: The giraffe's parameter.


Editorial: Evicting the mite.

Editorial: Evicting the mite.


Editorial: Epidemic giardiasis.

Editorial: Epidemic giardiasis.


Editorial: The Doctor Fox effect.

Editorial: The Doctor Fox effect.


Congenital and infantile neoplasia of the kidney.


Taking stock.

Taking stock.


Animal models in cancer research.

Animal models in cancer research.


Outbreak of psychosomatic illness at a rural elementary school.


Letter: Protein requirement.

Letter: Protein requirement.


Letter: Diabetes, HL-A antigens, and congenital rubella.


Letter: Units of energy in nutrition.

Letter: Units of energy in nutrition.


Letter: Diabetes and virus infection.

Letter: Diabetes and virus infection.


Letter: Incidence of diabetes in children.


Letter: Television in the ward.

Letter: Television in the ward.


Letter: Tight gloves.

Letter: Tight gloves.


Letter: Ovulation stimulation and defects of neural-tube closure.


Letter: Prospective diagnosis of spina bifida.


Letter: Diet and multiple sclerosis.

Letter: Diet and multiple sclerosis.


Letter: Anencephaly, subfertility, and abortion.


Letter: Effectiveness of hepatitis-B immune globulin in haemodialysis patients.


Letter: Dane particles in cord blood.

Letter: Dane particles in cord blood.


Letter: HBsAg subtype and chronic glomerulonephritis.


Letter: Risk of transfusing blood containing anti-HBs.


Letter: Absence of HBsAg and anti-HBs in spinal fluid.


Letter: Reaction to phenoxybenzamine.

Letter: Reaction to phenoxybenzamine.


Letter: Contamination of factor-VIII concentrates with metal particles.


Letter: Angina pectoris with normal coronary arteries.


Letter: Salicylates and pregnancy.

Letter: Salicylates and pregnancy.


Letter: Toxicity of chenodeoxycholic acid in rhesus monkeys.


Letter: Purity criteria for chenodeoxycholic acid.


Letter: Base excess and organic acidaemia.


Lactic acidosis and hyperlactataemia.

Lactic acidosis and hyperlactataemia.


Letter: Familial Hodgkin's disease.

Letter: Familial Hodgkin's disease.


Letter: Intercellular junctions between erythroblasts.


Letter: Frequency of cystic-fibrosis gene.


Letter: Lithium in thyrotoxicosis.

Letter: Lithium in thyrotoxicosis.


Letter: Fat cells in childhood obesity.

Letter: Fat cells in childhood obesity.


Letter: Menkes' kinky hair disease treated with subcutaneous copper sulphate.


Letter: Herpes rugbeiorum.

Letter: Herpes rugbeiorum.


Letter: Metabolic defect in hyperornithinaemia.


Letter: The consultants' contract.

Letter: The consultants' contract.


Letter: The "political health service".

Letter: The "political health service".


Letter: Social work and general practice.


Letter: The specialty of infectious disease.


Letter: Assisted entry to medical schools for health workers as mature students.


Letter: A XXX-XXY marriage.

Letter: A XXX-XXY marriage.


Letter: Distinguishing T and B lymphocytes by light microscopy and Normarski optics.


Letter: Colistin sensitivity testing.

Letter: Colistin sensitivity testing.


Cryosurgical treatment of recurrent head and neck malignancies-a comparative study.


Mitochondrial swelling associated with exercise and method of fixation.


Vascular reactions to laser in vivo.

Vascular reactions to laser in vivo.


Adrenergic innervation and vascular patterns in canine adipose tissue.


The cervical lymphatic ducts of the dog: structure and function of the endothelial lining.


Capillary pore rheology of erythrocytes. II. A method for the preparation of leucocyte-poor erythrocyte suspensions suitable for capillary pore rheometry.


On pericytes, particularly their existence on lung capillaries.


The place of chemotherapy in the treatment of advanced squamous carcinoma of the head and neck and in other situations.


A rapid silver protein method for nervous tissue.


Colour vision and the 'pill'.

Colour vision and the 'pill'.


Early interaction between animal viruses and cells.


Protection of mouse spermatogonia against x-ray induced translocations.


The histochemistry of hyaluronic acid and related mucosaccharides in the cerebral and other arteries of the dog.


Possible role of DNA synthesis in formation of sister chromatid exchanges.


Large numbers of cells in normal mice produce antibody components of isologous erythrocytes.


Histology of orofacial tumours perfused with bleomycin.


Immunocytochemical localization of dopamine-beta-hydroxylase in adrenal chromaffin granules.


The spinal cerebrospinal fluid absorptive pathways.


An improved rapid nuclear staining technique for permanent sections.


Neuropathologic aspects in Menkes' Kinky hair disease (trichopoliodystrophy). Menkes' Kinky hair disease.


Light microscopic staining and histochemical applications for plant meristems embedded in Spurr's medium.


Aspects of surgical nursing.

Aspects of surgical nursing.


Disclosing wafers in an oral hygiene instruction program.


Hodgkin's disease in the elderly.

Hodgkin's disease in the elderly.


Oral focal mucinosis. A clinicopathologic and histochemical study of eight cases.


Modifications of surface coat of aortic endothelial cells in hyperlipemic rats.


The ultrastructure of elastic tissue.

The ultrastructure of elastic tissue.


Corpora amylacea in parathyroids in cases of primary hyperparathyroidism.


A comparative analysis of the ultrastructure, biology and pathogenicity of mycoplasmas.


Epidemiology of organochlorine insecticides in the adipose tissue of Israelis.


Polychlorinated biphenyl and organochlorine pesticides residues in Canadian chicken eggs.


Care of the lower limb amputee.

Care of the lower limb amputee.


Isolation of plasma membrane from porcine spermatozoa.


Isoelectric focusing of peripheral lymphocytes.


Particles with characteristics of leukoviruses in cultures of marrow cells from leukemic patients in remission and relapse. An enzyme activity with the characteristics of RNA-directed DNA polymerase (reverse transcriptase) was detected in marrow from patients with leukemia in relapse and in firm hematological remission. Material having the enzyme activity, when analyzed in sucrose gradients, appeared as two distinct homogeneous bands of particles with densities of about 1.17 and 1.23 g/ml. The enzyme activity was stimulated by exogenous template poly(rC).(dG)(12-18) but not by (dT)(12-18). The enzyme activities in these bands also increased (1.7- to 24-fold) after culture, and both bands with enzyme activity were obtained from the cultured cells and from the supernatant medium. Electron microscopic studies showed that the two bands contained particles resembling leukoviruses or their cores.


Similar idiotypes in antibody-forming cells and in cells synthesizing immunoglobulins without detectable antibody function. The occurrence of immunoglobulins with and without antibody specificity and with and without idiotypic specificity was studied, by use of enzyme-labeled antigen and antibodies, in lymph node cells of rabbits immunized with horse-radish peroxidase and hen ovalbumin. Some cells, containing immunoglobulins without detectable antibody function, were shown to contain idiotypes similar to those found in antibody-producing cells.


Nature and mechanism of cellular recognition and regulation of leucocyte migration.


A manyfold increase in sister chromatid exchanges in Bloom's syndrome lymphocytes. Dividing cells from persons with Bloom's syndrome, an autosomal recessive disorder of growth, exhibit increased numbers of chromatid breaks and rearrangements. A highly characteristic feature of the chromosome instability in this syndrome is the tendency for exchanges to occur between chromatids of homologous chromosomes at homologous sites. In the present experiments, a cytogenetic technique by which the sister chromatids of a metaphase chromosome are stained differentially has been used to demonstrate a striking and possibly specific, but hitherto unrecognized, increase in the frequency with which sister chromatids also exchange segments. The cells were grown in bromodeoxyuridine and stained with 33258 Hoechst and Giemsa. Whereas phytohemagglutinin-stimulated lymphocytes from normal controls had a mean of 6.9 sister chromatid exchanges per metaphase (range 1-14), those from persons with Bloom's syndrome had a mean of 89.0 (range 45-162). Normal frequencies of sister chromatid exchanges were found in cells heterozygous for the Bloom's syndrome gene, and also in cells either homozygous or heterozygous for the genes of the Louis-Bar (ataxia telangiectasia) syndrome and Fanconi's anemia, two other rare disorders characterized by chromosome instability. In a differentially stained chromatid interchange configuration discovered during the study, it was possible to determine the new distribution of both sister and non-sister-but-homologous chromatids that had resulted from numerous exchanges. By following shifts in the pattern of staining from chromatid to chromatid, visual evidence was obtained that the quadriradial configurations long recognized as characteristic of Bloom's syndrome represent exchanges between homologous chromosomes, apparently at homologous points. We postulate that the increase in the frequency of exchanges between nonsister-but-homologous chromatids and those between sister chromatids in Bloom's syndrome represents aspects of one and the same disturbance. A study of this phenomenon in relation to the clinical features of Bloom's syndrome may be helpful eventually in understanding the biological significance of chromatid exchange in somatic cells.


Sarcoma-virus-related RNA sequences in normal rat cells. A rat type C virus spontaneously activated from the NRK (normal rat kidney) cell line was found to have two major size classes of viral RNA subunits sedimenting at 35 and 30 S. Virus-producing cells contained both RNA species, while normal "virus-free" rat cells contained primarily virus-specific 30S RNA species. A DNA transcript, specific for Kirsten sarcoma virus, prepared from virus activated in nonproducer BALB/c cells originally transformed by Kirsten sarcoma virus and rendered specific for the virus by absorption of sequences related to mouse helper virus hybridized only with the 30S RNA species of virus-producing rat cells and normal rat cells. These findings are consistent with the hypothesis that sarcoma-specific nucleic acid sequences in kirsten sarcoma virus emerged through a process that incorporated some portions of 30S RNA species from rat cells (either normal or virus-producing) into the Kirsten leukemia virus during passage in vivo of that virus. The virus designated M-MSV(RaLV), which originally derived from tumor induced by Moloney sarcoma virus (M-MSV) in rats, contained 35S RNA species of rat type C viruses and 30S RNA species specific for both rat and mouse viruses. It appears striking that for these two animal species, sarcoma-virus-specific information resides on a 30S subunit.


Localization of Escherichia coli ribosomal proteins S4 and S14 by electron microscopy of antibody-labeled subunits. Binding sites for antibodies specific for proteins S4 and S14 of the small subunit of E. coli ribosomes have been mapped on the surface of the subunit by electron microscopy. Antibody binding to reconstituted subunits was shown to depend specifically on the presence of E. coli S4 and S14. Anti-S14 IgG was found to bind to a limited region of the ribosome surface. In contrast anti-S4 IgG was found to bind to three separated regions of the ribosome surface, suggesting S4 has an elongated conformation in situ.


Type C viral antigens in man. I. Antigens related to endogenous primate virus in human tumors. Partially purified extracts from 33 human tumors of several histologic types were used as competing antigens in a radioimmunoassay for the p30 protein of an endogenous primate type C virus (M7). Antigens immunologically related to the p30 protein of the M7 virus were detected in two tumors. Like viral p30 antigens previously identified in tissues of several other primates, the antigens found in human tumors crossreact with the p30 protein of the feline RD-114 virus but are unrelated by similar immunologic criteria to the p30 proteins of several other mammalian type C viruses. Gel filtration shows that most of the antigenic activity co-chromatographs with authentic p30 protein. These results, along with those showing nucleic acid sequences related to those of an endogenous primate type C virus in the DNA of human cells, make it clear that humans, like other primates, have type C viral sequences in their genome and can, in some circumstances, express at least one type C viral protein.


The identification of the eukaryotic ribosomal proteins homologous with Escherichia coli proteins L7 and L12. Antibodies were raised against eukaryotic (rat liver) and prokaryotic (E. coli) ribosomal particles and ribosomal proteins. The antisera were characterized and used to determine the identity of the eukaryotic proteins homologous to E. coli L7 and L12. The large subunit of rat liver ribosomes contains two acidic proteins, L40 and L41; they migrate during two-dimensional polyacrylamide gel electrophoresis in a way that mimics the behavior of L7 and L12. Rat liver L40 and L41 were found to be immunologically related to E. coli L7/L12, hence the proteins are likely to share some structural homology.


Actin is the naturally occurring inhibitor of deoxyribonuclease I. Various tissues and cells in culture contain a specific inhibitor of DNase I (EC 3.1.4.5). In this paper evidence is presented that this inhibitor is actin, one of the major structural proteins of muscle and nonmuscle cells. (a) The inhibitor is a major cellular component constituting 5-10% of the soluble protein. (b) It migrates with actin on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, having a characteristic molecular weight of 42,000. (c) It has an amino-acid composition closely similar to that of actin. (d) The peptide maps of the two proteins are nearly identical. (e) Skeletal muscle actin inhibits the enzymatic activity of DNase I. (f) DNase I-agarose affinity chromatography quantitatively retains purified skeletal muscle actin, and actin, specifically, from high-speed supernatants of whole cell extracts. (g) An antibody to purified inhibitor protein from calf thymus, used in indirect immunofluorescence on cells grown in culture, stains a two-dimensional network of fibers similar to that seen with an actin-specific antibody.The observation that actin can be isolated by DNase-agarose affinity chromatography provides a useful tool for the biochemical study of actin under different physiological conditions.


The regulation of protein synthesis in mammalian cells VI. Soluble and polyribosome associated components in controlling in vitro polypeptide initiation in HeLa cells. The in vitro initiation of polypeptides on endogenous polyribosomes has been studied in extracts from HeLa cells. Regulation of the rate of initiation of polypeptides can be examined. In these experiments an assay using [(35)S]fMet-tRNA(f) (Met) has been developed, and the system further characterized. The system has been separated into a fraction containing polyribosomes with subunits and a fraction containing soluble components. The regulation of initiation has at least two distinct components. There is one factor in the soluble fraction which develops a stimulated response after protein synthesis has been inhibited in intact cells. This stimulation does not require new RNA synthesis during the period of cell "stress."A second component is associated with ribosomes. This factor is necessary for the initiation of polypeptides on endogenous polyribosomes. It disappears gradually when cells are exposed to actinomycin. The disappearance is first manifested by an inability of polyribosomes to respond to stimulated supernatants. This unstable component, which decays in the presence of actinomycin, has no apparent counterpart in systems that measure initiation on exogenous mRNA.


Alterations of membrane glycopeptides in human colonic adenocarcinoma. Membrane glycopeptides were examined in human colonic adenocarcinoma and normal colonic mucosa. The carbohydrates of membrane glycopeptides were found to be markedly reduced in tumor tissue and the relative proportions of the various sugars were altered. Although all of the sugars were lower in tumor tissue when compared to the adjacent normal mucosa, galactosamine, fucose, and sialic acid were more significantly reduced. Examination of the blood group activity and lectin-binding properties of membrane glycopeptides revealed that specific carbohydrate structures had changed in the tumor tissue. Most striking of these changes was the disappearance of glycoprotein-associated blood group A activity. Assay of the enzyme responsible for synthesis of the blood group A determinant showed that this glycosyltransferase activity was greatly diminished in tumor tissue. A galactosyltransferase and a fucosyltransferase were also significantly lower in the tumor tissue whereas the levels of another galactosyltransferase and a sialyltransferase were unaltered. Glycosidase activities in the normal and tumor tissues were similar. The results show that an alteration in glycoprotein biosynthesis occurred during tumorigenesis that resulted in a modified membrane glycoprotein composition and that these changes are probably a reflection of reduced levels of the enzymes responsible for glycoprotein synthesis.


Reverse transcriptase: correlation of zinc content with activity. Evidence is presented that DNA polymerase of avian myeloblastosis virus has an obligatory zinc requirement for activity. Previous studies indicate that the purified polymerase contains zinc in a stoichiometry of about 1 g-atom/mole. We now find that the enzyme-bound zinc is exchangeable with radioactive (65)Zn; after isoelectric focusing, the radioactive (65)Zn is coincident with polymerase activity. Dialysis of the (65)Zn-labeled polymerase against the chelator, 1,10-phenanthroline, results in a progressive loss of radioactive (65)Zn and polymerase activity. Thereupon, incubation of the inactivated enzyme with Zn(2+) fully restores activity. Thus, the DNA polymerase present in an oncogenic RNA virus, like animal DNA polymerases, can be rigorously classified as a zinc metalloenzyme. DNA polymerase of avian myeloblastosis virus is inactivated by 1,10-phenanthroline at a much faster rate than the bacterial and animal DNA polymerases that have been tested. It may, therefore, be possible to inactivate selectively DNA polymerases from animal tumor viruses by brief exposure to appropriate metal chelators.


Studies on the reverse transcriptase of RNA tumor viruses. Structural relatedness of two subunits of avian RNA tumor viruses. The structural relationship of the small (alpha) and large (beta) subunits of reverse transcriptase isolated from two avian RNA tumor viruses has been examined by tryptic peptide analysis. Comparison of the tryptic hydrolysates of the isolated subunits by two-dimensional separation on thin-layer cellulose plates indicates that (i) the alpha subunit of reverse transcriptase of avian myeloblastosis virus is structurally related to the beta subunit; (ii) the alpha and beta subunits of the enzyme of Rous sarcoma virus also appear to be related; and (iii) there appears to be an extensive amino-acid sequence homology between reverse transcriptases of avian myeloblastosis virus and Rous sarcoma virus. Evidence is also presented that both alpha and beta subunits can be identified in purified avian myeloblastosis virions.


The presence of I-region-associated antigens on B cells in molecules distinct from immunoglobulin and H-2K and H-2D. The distribution of I-region-associated antigenic specificities in murine lymphoid cells was studied by immunofluorescence. The antigens were mostly represented in B cells and in macrophages. The complexes of I-anti-I-region-associated antigens were found to be distributed throughout the lymphocyte surface both at 4 degrees and 37 degrees . In about two-thirds of the lymphocytes the complexes redistributed into caps after incubation with a second ligand (an anti-mouse immunoglobulin). The I-region specificities were in molecules distinct from immunoglobulin or from the molecules coded by the H-2K and H-2D regions.


Nonhistone proteins control gene expression in reconstituted chromatin. Chromatin was reconstituted from the purified DNA and histones of chicken erythrocytes and the nonhistone proteins of either chicken reticulocytes or chicken liver. Reconstituted chromatins, native chicken reticulocyte chromatin, and free DNA were transcribed with Escherichia coli RNA polymerase and the concentrations of globin-specific sequences in the RNA products were measured by hybridization with [(3)H]DNA complementary to chicken globin messenger RNA. Reticulocyte, but not liver, nonhistone proteins were shown to activate the globin genes in reconstituted erythrocyte chromatin. The transcripts of native and reconstituted chromatins were indistinguishable in respect of both the total yield of the RNA and the fractional yield of globin-specific sequences.


Solitary plasmacytoma of the clavicle.

Solitary plasmacytoma of the clavicle.


Regulation of immune response by autogenous antibody against receptor. BALB/c mice repeatedly immunized with Pneumococcus R36A vaccine produce antibodies to phosphorylcholine having the TEPC-15 myeloma idiotype (murine IgA myeloma protein that binds phosphorylcholine). The plaque-forming cell response to phosphorylcholine shows a decrease with repeated immunizations. In contrast, spleen cells from multiply immunized mice responded better in vitro than spleen cells from nonimmunized mice. The serum of animals immunized four or five times agglutinates TEPC-15-coated sheep erythrocytes. Inhibition of hemagglutination shows that the agglutinating activity is directed against the TEPC-15 idiotype. Sera from these mice, when added to cultures of normal spleen cells, specifically suppress the response to phosphorylcholine. The suppressive activity in the serum can be removed by solid absorption with TEPC-15. Evidently, repeated immunization with antigen induces two kinds of antibody responses: one directed against antigen and the other directed against the antibody to the antigen. It is proposed that this "auto" antibody against receptor is involved in the regulation of the immune response.


Measles vaccines.

Measles vaccines.


Mixed lymphocyte culture determinants, measles infection and multiple sclerosis.


Platelet aggregation and release triggered by antibody to platelet factor 4 (PF4).


Anaphylactoid edema induced in rats by nickel and cobalt salts.


Induction of guinea pig leukemia-like virus from cultured guinea pigs cells.


Determination of uterine blood volume and correlation with ovarian function.


Comparative effects of pancuronium and d-tubocurarine on blood histamine and sympathetic activity.


The effects of thyroid state on rat liver glucose-6-phosphatase activity and glycogen content.


Microtubules in prolactin cells of the rat anterior pituitary gland.


Effect of con A on tolerance to BGG in NZB-W mice.


Suppression of prolactin secretion by acute administration of delta9-THC in rats.


Chromosome changes revealed by the Q-band staining method during cell senescence of WI-38.


Drug displacement from protein-binding and redistributional interactions.


Metabolic side-effects of allopurinol.

Metabolic side-effects of allopurinol.


Crystalline glycoprotein cell walls of algae: their stucture, composition and assembly.


Experimental study of the regeneration of sympathetic nerve fibers after nerve homografting.


Some electron microscopic studies on intact nuclear 'ghosts' and nuclear membrane fragments.


Mechanisms of immunological enhancement.


Chemical pollutants with special reference to sources of pollution.


Alpha-fetoprotein: expression in human disease and in rat experimental models.


Tumours of liver, biliary system and pancreas.


Classification of acute leukaemias using Romanowsky, sudan black and periodic acid Schiff stains.


Introduction to nomenclature and classification of acute leukemias.


Practical value of cytochemical studies for the classification of acute leukemias.


Indications and limits of cytochemistry in acute leukemia.


Reaginic antibody in experimental allergic encephalomyelitis. I. Characterization of heat-labile skin-fixing antibody.


Effects of chronic ingestion of some lower aliphatic alcohols in rats.


Localization of narcotic analgesics in synaptic membranes of rat brain.


Reaginic antibody in experimental allergic encephalomyelitis. II. Histamine and serotonin blockade.


Histochemical study of the formation of dental plaque in artificial fissures.


A national therapy project for drug resistant pulmonary tuberculosis.


Pachytene analysis of acrocentric human chromosomes.


Radioimmunoassay for gamma-a fetal protein in serum of patients with cancer and nonmalignant diseases.


Oxygen consumption by acrylamide polymerization for rapid screening of chemotherapeutic agents.


Interdisciplinary management of developmental disabilities cases.


Corneal nerves.

Corneal nerves.


Lack of association between carcinoma of the breast and HL-A specificities.


Inhibition of the mixed lymphocyte reaction by sera from multipara.


Subacute thyroiditis: observations on 98 cases for the last 14 years.


Pertinent features of M locus determinants including revised nomenclature and strain distribution.


Influence of phosphororganic radioprotective drugs on postirradiation changes of reactivity to barium chloride in guinea pig intestine.


Rabbit anti-human thymocyte globulin (RAHTG): in vitro and in vivo testing.


Genetics of the human histocompatibility system HL-1.


Evaluation of the efficiency of leukocyte transfers by Y-chromatin studies.


Typing for the MLC determinants and its possible impact on Europdonor.


Cross-reactive antigens, cancer, and transplantation.


Immunological enhancement of renal allografts.


Human prostatic reverse transcriptase and RNA-virus.


Isolation of glomeruli and tubular fragments from rabbit kidney.


Intracisternal hyalin in hepatocytes of human liver biopsies.


Distinguishing normal human blood lymphocytes from lymphocytes in dengue and typhoid fever.


Polypeptide hormone production by "carcinoid" apudomas and their relevant cytochemistry.


Nursing in Padang, Indonesia.

Nursing in Padang, Indonesia.


Electrophoretic examination of cerebrospinal fluid proteins in children with acute lymphocytic leukemia. II. Communication.


Changes of the protein composition of the cerebrospinal fluid in childhood acute leukemia.


Studies on the application of 125I labelled testosterone in protein binding methods.


Routine procedure for the separation of the isoenzymes of alkaline phosphatase.


Experimental ependymomas: in vivo and in vitro morphology.


Histamine and serotonin in the gastric erosions of rats dead from exposure to cold: a histochemical and quantitative study.


Review of the global epidemiological situation of plague since the last Congress of Tropical Medicine and Malaria in 1968.


Studies on the antigenic determinant group of the type-specific antigen of Leptospira canicola.


Acid phosphatase from human prostate. Chemical modification and activity of the enzyme.


Open heart surgery in the first year of life.


Fluorometric characterization of six classes of human leukocytes.


Studies on guinea pig skin cell cultures. IV. Collagen synthesis.


An improved method for the preparation of epidermal sheets.


Participation of singlet state excites oxygen in photohemolysis induced by kynurenic acid.


Ultrastructure of necrobiosis lipoidica diabeticorum.


Cytological effects of thio-TEPA on ascites tumors in mice.


Membrane properties of lymphatic cells in fine needle biopsies of lymphomas. I. Cap formation after exposure to fluorescein conjugated concanavalin A.


Immunological tolerance and dermal eosinophilia induced in dinitrochlorobenzene-sensitized guinea pigs.


A new technique for colonic cytology.

A new technique for colonic cytology.


HL-A-antigens and psoriatic arthritis.

HL-A-antigens and psoriatic arthritis.


Correlative study of rubella antibody titre, basement zone antibody titre, and IgG content of sera of patients with bullous pemphigoid.


Precipitating antibodies to sulfathiazole in serum of patients with suspected allergy to sulfonamides.


Studies on tryptophan catabolism in acrodermatitis enteropathica.


Some cytochemical properties of isolated human endosalpingeal cells.


Hematological changes in psoriasis. Secondary anemia, XVIII.


Cytologic diagnosis in primary carcinoma of Fallopian tube. Case report and review.


Occupational and non-occupational scrotal cancer in Sweden, 1958-1970.


Hyperkeratosis lenticularis perstans (Flegel).


On the occurrence of genital herpes simplex virus infection. Clinical and virological findings and relation to gonorrhoea.


Effects of long term treatment with tetracycline.


Treatment of light sensitivity with carotenoids. Serum concentrations and light protection.


Quality evaluation of cytology laboratories in New York State: expanded program, 1971-73.


Letter: Etiology of erythropoietic protoporphyria, and protection of photosensitive subjects.


Changes in the level of immunoglobulins in the Danzig quintuplets in their first year of life.


Observation of the cells of basophilic series by Astra blue staining.


Staining of cholesterol with the fluorescent antibiotic "filipin".


On the structure and histochemistry of the cuticle of austroperipatus Peripatoides novae zealandiae.


Urea and pyridine in Feulgen staining of Bryophyllum.


Ruthenium red and lanthanum nitrate a possible tracer and negative stain for scrapie "particles"?


The growth of the cranial base in the Wistar albino rat studied by vital staining with alizarin red S.


Studies on the factors inducing metastasis of breast cancer to lymph node. I. Lymph flow in the thoracic wall.


Measles virus antibodies in multiple sclerosis.


Taxonomic criteria for Mycobacteria and nocardiae.


Human experiences related to adverse drug reactions to the fetus or neonate from some maternally administered drugs. This is a review of known periods in utero during which drugs alter the process of growth; effects may be shown on the fetus or the newborn and vary with the stage of development of the fetus when exposed.  Other variables are the mother and the placenta.  There is no safe animal screening mechanism, the human test is by ordeal, and more clinical monitoring and reporting are needed.  Cancer chemotherapeutic agents exert their maximal effects on rapidly dividing cells and are therefore hazardous during pregnancy; the greatest risk is in the 1st trimester.  In the thalidomide experience the critical days were the 22nd and 23rd days after conception.  Masculinizing drugs such as testosterone and other androgenic steroids have been implicated as affecting the female fetus when given early in pregnancy.  Oral contraceptives taken by an already pregnant woman are a hazard because of these progestational agents.  Progesterone alone is unlikely to cause masculinization but other progestins may cause such changes.  Carcinogenesis may develop later in females born of mothers who are treated with diethylstilbestrol to prevent miscarriage.  Many antithyroid drugs have caused neonatal goiter.  Maternal ingestion of iodides during pregnancy (preparations for treating asthma, cough syrups, radio-contrast media used in diagnoses) is the most frequent cause.  Goiter is relatively common in infants whose mothers were treated with propylthiouracil and other antithyroid drugs, yet they usually show normal thyroid function.  However, hypothyroidism with cretinism can occur.  Lithium, used in psychiatry and as a salt substitute, may alter iodine metabolism and thyroid gland function.  It also passes into the milk to continue the potential toxicity.  Teratogenic effects in experimental animals suggest other possible effects on infants from lithium and similar drugs.


Cytochemical studies of secretory and other granules associated with the endoplasmic reticulum in rat thyroid epithelial cells.


Transmitter release and recycling of synaptic vesicle membrane at the neuromuscular junction.


Stereological study of pancreatic exocrine cells.


Activation of mammalian leukemia viruses.


Structural components of rna tumor viruses.


Localization of granules stained artifactually with the Fink-Heimer technique in certain brain stem nuclei of the pigeon.


Dominant piebald trait in a retarded child with a reciprocal translocation and small intercalary deletion.


Viral potentiation of chemical toxins in the experimental syndrome of hypoglycemia, encephalopathy, and visceral fatty degeneration.


A familial X-22 translocation with an extra X chromosome.


Staining of glutaraldehyde fixed, glycol methacrylate embedded hamster lungs.


Declining testicular function with age. Hormonal and clinical correlates.


Timely topics in clinical chemistry: in vitro thyroid testing.


Pericentric inversions of human chromosomes 9 and 10.


Evaluation of a new automated tissue stainer.


Effect of adrenergic blockade and specific antithyroid therapy on electrocardiographic changes in hyperthyroidism.


A light and electron microscopic study of anterior subcapsular cataracts.


Clinical, pathologic, and therapeutic aspects of carcinoma of the pancreas.


Junctions between the cells of the trabecular meshwork.


Some morphological features of the myoneural junctions in certain normal muscles of the rat.