Letter: Growth-hormone response to exercise in obese children.


Letter: Postoperative gynaecological wound infection.


Letter: Immunotherapy with intravenous B.C.G.


Letter: Blood fractions for treatment of haemophilia.


Letter: Cell-mediated immunity in hepatitis.


Letter: Prolactin levels in gynaecomastia related to Klinefelter's syndrome.


Letter: Turner's syndrome and long-arm deletions of X chromosome.


Letter: Effect of clonidine on nicotinic-acid flushing.


Letter: Geriatrics is medicine.

Letter: Geriatrics is medicine.


Obituary. John George Gibson.

Obituary. John George Gibson.


Leukemic infiltration of prostate.

Leukemic infiltration of prostate.


The innervation of the salivary gland of the moth, Manduca sexta.


Ultrastructural distribution of cell surface antigens in avian tumor virus-infected chick embryo fibroblasts. The distribution of neoantigens in the surface membrane of avian tumor virus-infected chicken embryo fibroblasts was examined on carbon replicas of cell cultures using hemocyanin-labeled antibody. New determinants appearing on the cell surface of virally infected but not transformed cells are thought to be common with components of the viral envelope. These antigens were found to exist in a diffuse, random array on the dorsal cell surface, with a denser accumulation along the cell processes. In living cells, surface antigens are capable of several types of redistribution when activated by reaction with antibody. Leukosis virus-infected (non-transformed) cells showed two apparently independent modes of redistribution: a relocation of some antibody-related sites to the cell margin; or an involvement of essentially all sites in randomly dispersed aggregates. Viral antigenic sites on sarcoma virus-infected (transformed) cells, reacted with antibody, were able to produce weak marginal relocation; but revealed a more striking tendency to migrate to some central location. The centripetal coalescence thus formed resembles the "cap" noted in other systems. Prior aggregation into "patches" may not be a prerequisite for such cap formation. Tumor-specific surface antigen detection and mapping was attempted by this technique, but results were equivocal. An antigen possibly characteristic of rapidly dividing cells occurred in a sparse, diffuse fashion over the surface of morphologically distinct "round" cells.


Temperature dependence of mast cell histamine secretion.


Alpha1-fetoprotein biosynthesis during the growth cycle of differentiated fetal rat hepatocytes in primary monolayer culture.


Physical and chemical properties of an oncornavirus associated with a murine adrenal carcinoma cell line. A type C oncornavirus has been isolated from a continuous cell line of murine adrenal carcinoma in culture. The particles have a buoyant density of 1.165 g/cm(3), exhibit typical type C morphology by electron microscopy, possess an RNA-dependent DNA polymerase, and have a high molecular weight RNA (6.1 x 10(6)) which can be denatured to a homogeneous lower molecular weight species (3.2 x 10(6)) when extracted from rapidly harvested "immature" virions. The virus is related antigenically to other mammalian oncornaviruses and exhibits a similar, although much more complex, sodium dodecyl sulfate gel electrophoretic profile of virion proteins when compared to the profiles of other type C RNA tumor viruses.


Isolation and characterization of RNA-directed DNA polymerase from a B-type RNA tumor virus. RNA-directed DNA polymerase was isolated from milk-borne B-type murine mammary tumor virus of the RIII mouse strain. The several hundred-fold-purified enzyme sediments at 5.5 to 5.7S with an average molecular weight of approximately 100,000. The purified enzyme is completely template dependent and responds to RNA, DNA, and synthetic templates. Stability studies indicate differential lability dependent on the exogenous template used to monitor activity.


Immunological properties of two polypeptides of Mason-Pfizer monkey virus. Two polypeptides were isolated from the Mason-Pfizer monkey virus (MP-MV). The polypeptides, designated MP-MV p26 and MP-MV p15, were 26,000 and 15,000 molecular weight, respectively, based on gel filtration chromatography in the presence of 6 M guanidine hydrochloride. Radioimmunoassays developed for MP-MV p26 and p15 were, respectively, 10 and 100 times more sensitive than immunodiffusion and 1 and 10 times more sensitive than micro-complement fixation for the detection of MP-MV. Antigens of other reverse transcriptase-containing RNA viruses did not cross-react in either MP-MV radioimmunoassay. Further, antisera against these other viruses did not react with the radioiodine-labeled MP-MV polypeptides. The MP-MV radioimmunoassays should be useful in studying the natural occurrence of this virus and its relationship to primate tumors.


Introductory remarks for The Third James A. English Symposium.


Deficiency of 60 to 70S RNA in murine leukemia virus particles assembled in cells treated with actinomycin D. Production of particles with the ultrastructural appearance of C-type virions persisted for at least 6 h in actinomycin D-treated cells infected with murine leukemia virus. This phenomenon occurred despite severe inhibition of viral RNA synthesis. Virus particles present in a 6-h harvest sedimented in sucrose gradients with the buoyant density characteristic of RNA tumor viruses (1.16 g/cm(3)) and exhibited high levels of reverse transcriptase activity in response to the exogenous template polyriboadenylic acid.oligo deoxythymidylic acid in the range of untreated controls. However, RNase-sensitive endogenous activity was only (1/5) the level found in controls. This observation correlated with a marked reduction in infectivity. Kinetic studies on the appearance of labeled RNA in banded virions revealed that within the first hour after addition of actinomycin D, particles contained 60 to 70S RNA and two low-molecular-weight RNA species corresponding to 8 and 4S RNA. After approximately 1 h of incubation with actinomycin D, 60 to 70S RNA could not be detected and 4S RNA was the predominant species. These findings suggest that murine leukemia virus particles assembled in the presence of actinomycin D are deficient in 60 to 70S viral RNA.


Structural proteins of mammalian oncogenic RNA viruses: murine leukemia virus neutralization by antisera prepared against purified envelope glycoprotein. Goat and rabbit antisera prepared against a purified Rauscher murine leukemia virus glycoprotein (gp69/71) rapidly neutralized spleen focus-forming virus in Rauscher and Friend virus preparations. Absorption studies revealed that most of the neutralizing activity of goat anti-Rauscher virus gp69/71 serum was directed against type- and group-specific determinants.


Street runoff as a source of lead pollution.


The recognition of homologous and heterologous erythrocytes by guinea pig macrophages.


Immunologically active glycopeptides derived from CEA.


Relationship of skin reactive intestinal cancer antigen to the carcinoembryonic antigen of Gold and other antigens.


Specificity of the carcinoembryonic antigen.


Staining of biological material for the scanning electron microscope.


Review of the biochemistry and coagulation physiology of brinolase. (Fibrinolytic enzyme from Aspergillus oryzae).


Recent findings concerning dose response relationship in mutagenicity testing of chemicals.


Studies on the proteolysis of fibrinogen and fibrin by Aspergillus ochraceus enzyme as compared to the action of plasmin.


Histochemical studies of cartilage development in the mouse limb-bud. I. Connective tissue, RNA, and protein end-group methods.


Immunology of the gastrointestinal tract. II.


Optical polarisation indicates linear arrangement of rhodopsin oligosaccharide chain in rod disk membranes of frog retina.


Measurement of serum folate levels and serum folic acid-binding protein by 3H-PGA radioassay.


An improved nitroblue tetrazolium test and its correlation with toxic neutrophils.


5-Bromo-2'-deoxyuridine potentiation of transformation of rat-embryo cells induced in vitro by 3-methylcholanthrene: induction of rat leukemia virus gs antigen in transformed cells. Low-passage rat-embryo cells were not transformed by 3-methylcholanthrene or by 5-bromo-2' deoxyuridine. However, prior treatment with bromodeoxyuridine, followed by treatment with methylcholanthrene, resulted in cell transformation about three subpassages after removal of the carcinogen. RNA-directed DNA polymerase activity could not be detected in either normal or transformed cells. However, gs-1 antigen specific for rat C-type RNA virus was detected in cultures derived from bromodeoxyuridine-treated cells. No gs-1 antigen for the C-type RNA virus was detected in cultures that had not been treated with bromodeoxyuridine during the 25 subpassages of these experiments.High-passage rat-embryo cells, derived from a different cell pool, were transformed by either methylcholanthrene or dimethylbenzanthracene without prior infection with an exogenous virus, and without prior treatment with bromodeoxyuridine, gs-1 antigen for C-type RNA virus was also detected in 4 of 4 randomly selected transformed cell lines; the gs-1 antigen was not detected in any of 4 nontransformed control cultures. Considering these and previously published findings, we conclude that the lowpassage cells cannot be transformed by methylcholanthrene because of powerful cellular controls over the endogenous virus. Bromodeoxyuridine triggers some expressions of the endogenous virus; thus, the bromodeoxyuridine-treated cells are more susceptible to the transforming effects of methylcholanthrene. High-passage rat cells do not maintain perfect control over expression of their endogenous virus; the cell cultures are susceptible to the transforming effects of chemical carcinogens.


Letter: Celestin blue B and hematoxylin.


Hepatic cytopathology in alpha-1-antitrypsin (alpha-1-AT) deficiency.


Cystic dilatation of sex accessory organs in common and inbred Syrian Hamsters.


Expression of the defective "S plus L-" type murine sarcoma virus genome in human amnion and lung cells.


In vivo growth and antigenic properties of a rat sarcoma induced by Moloney sarcoma virus.


Enhanced production of mouse mammary tumor virus in dexamethasone-treated, 5-iododeoxyuridine-stimulated mammary tumor cell cultures.


HL-A heterozygosity as a genetic marker of long-term survival.


A new routine in histopathology.

A new routine in histopathology.


Mitomycin-induced lysis of Clostridium sordellii.


The kinetics of erythrocyte lysis by Escherichia coli haemolysin.


Formation of "amyloid" fibrils in vitro by action of human kidney lysosomal enzymes on Bence Jones proteins.


Gastric and caecal lesions in pigs following the feeding of sulphite.


Experimental Escherichia coli mastitis in cattle, its pathogenisis and treatment.


Biosynthetic activities of dorsal root ganglia in vitro and the influence of nerve growth factor.


Low-angle x-ray diffraction studies on the period of central myelin sheaths during preparation for electron microscopy. A comparison between different anatomical areas.


Fast and slow contraction times associated with fast and slow spike conduction of skeletal muscle fibres in normal subjects and in spastic hemiparesis.


Electroanatomical observations on the ventro caudal parts of the thalamus according to the facts of stereotactic stimulation in man.


Rubella.

Rubella.


Effects of supraoptimal temperatures on the myxomycete Physarum polycephalum. II. Effects on the rate of protein and ribonucleic acid synthesis.


Cryoglobulins in glomerulonephritis not related to systemic disease.


The metabolism of porphyrins of the isocoproporphyrin series.


The determination in human males of specific testosterone and 5aplha-dihydrotestosterone binding to sex hormone binding globulin by a differential dissociation technique.


Synchrotron radiation and its applications in biology.


Relation of absorption rates of glucose, glycine and glycylglycine to serum globulin concentrations in man in vivo.


Involvement of IgE in con A-induced histamine release from human basophils in vitro.


The effect of nerve growth factor (NGF) upon axoplasmic transport in sympathetic neurons of the mouse.


Subcellular localisation of adrenal cholesterol by autoradiography and digitonin reaction after aminoglutethimide-induced inhibiton of corticosterone synthesis.


Ultrastructure of exogenous peroxidase in cerebral cortex.


Evidence for PAS positive reaction of the corticotroph granules of the rat adenohypophysis.


Rohde's fiber: a septate axon in the leech.


Cytoplasmic order in an invertebrate neuron.


The deficient density-dependent growth control of human malignant glioma cells and virus-transformed glia-like cells in culture.


Expression of fetal antigens and tumor-specific antigens in SV40-transformed cells. I. Serological analysis of the antigenic specificities.


Some observations on the skeletal musculature of aged rats. I. Histological aspects.


A new approach for studies on calcareous corpuscles in Hymenolepis microstoma.


The HL-A system. II. Clinical relevance.


Ontogenesis of sympathetic fibers after neonatal 6-hydroxydopamine treatment in the rat.


The cellular recognition in vitro of antigens related to human histocompatibility.


Neutrophil-specific antigens: immunology and clinical significance.


Carcinoma of the lip. Analysis of the material of 25 years.


Absence of B antibody in a blood group A person.


Combined intervention for controlling unwanted homosexual behavior.


Morphological studies of Herpesvirus saimiri in primate cell cultures.


A molecular theory to explain the mechanisms of allergic histamine release.


The morphology of Cryptococcus neoformans in human cryptococcosis. A light-,phase-contrast and electron-microscopic study.


Neurosecretion in the pineal gland of Macaca rhesus.


Aqueous solution of acridine orange in the staining of DNA-aldehyde.


The aid of some histochemical stains in the identification of mesothelial cells. Preliminary results.


Chronic plasma cell cervicitis. A rare pitfall in gynecological cytology.


Synergia of a heparinoid and promethazine hydrochloride against liver cell necrosis.


Diagnosis of strongyloidiasis in Papanicolaou-stained sputum smears.


The effects of age and exercise on the succinic dehydrogenase content of individual muscle fibres from fast, slow and mixed hamster muscles.


Prevention by aging or by cytochalasin B of phalloidin stimulated formattion of microfilaments in cell membrane preparations of rat liver.


Glucose-6-phosphatase heterogeneity within the hepatic lobule of the phenobarbital-treated rat.


A description by computer of texture in Wright-stained leukocytes.


Peroxidase and amylase activity of the guinea pig submandibular gland during the secretory cycle. An electronhistochemical and biochemical study.


Expression of fetal antigens and tumor-specific antigens in SV40-transformed cells. II. Tumor transplantation studies.


Expression of the major group-specific antigen (GS-A) of avian type-C viruses in normal chicken cells and tissues.


Histochemistry of primate extraocular muscles and the changes of denervation.


Effect of chronic sulfur dioxide inhalation on the carbohydrate histochemistry and histology of the canine respiratory tract.


An outer membrane protein of Neisseria meningitidis group B responsible for serotype specificity. Meningococcal groups B and C have been subdivided into a series of serotypes based upon the antigenic specificity of protein serotype antigens (STA). The purpose of these studies was to obtain the STA by gentle methods and determine its anatomic location in the meningococcal cell. The STA was extracted from group B meningococcal strains by either 0.2 M LiCl or 0.2 M CaCl(2) and isolated from the extracts by gel filtration on Sepharose 6B or by pelleting the STA by centrifugation at 100,000 g. The isolated STA was a lipoprotein-lipopolysaccharide complex with a mol wt of approximately 4 x 10(6) daltons. Antisera prepared against the type 2 STA were bactericidal only for homologous serotype strains. The STA proved to be a constituent of the outer membrane of the cell envelope. This was shown by SDS-polyacrylamide gel electrophoresis (PAGE) of the isolated outer membrane and of the purified STA. The type 2 STA complex contains three principal proteins, one of which is predominant with a mol wt of 41,000 daltons. The type 2 STA was dissociated by Triton X-100 and separated by sucrose gradient isodensity centrifugation into two peaks. The denser peak (rho = 1.26 g/cm(3)) contained the majority of the 41,000 dalton major outer membrane protein as shown by SDS-PAGE. This peak also contained the type 2 antigenic determinant. Thus the major outer membrane protein, extracted as part of a lipoprotein-lipopolysaccharide complex, contains the type 2 STA determinant.


Degradation of dyfonate in soil inoculated with Rhizopus arrhizus.


A modified assay for the Waaler-Rose test using latex particles coated with rabbit gamma-globulins.


Cutaneous localization of human gamma-globulins in lupus erythematosus. An electron-microscopical study using the peroxidase-labeled antibody technique.


Incorporation of exogenous DNA into mammalian chromosomes.


A glycogen-rich segment of the tubuli recti and proximal portion of the rete testis in the guinea-pig.


Proteins and amino acids in bovine oviducal fluid.


Staphylococcal heat-stable opsonins.

Staphylococcal heat-stable opsonins.


Fractionation of bee venom. I. Preparation and characterization of four antigenic components.


The exudative lesions in diabetic retinopathy with special regard to the hard exudate.


Spontaneous regression of a malignant melanoma of the choroid.


Benign mucous membrane pemphigoid. 1. Secretion of mucus and tears.


Benign mucous membrane pemphigoid. II. Cytology.


Differential light scattering from spherical mammalian cells. The differential scattered light intensity patterns of spherical mammalian cells were measured with a new photometer which uses high-speed film as the light detector. The scattering objects, interphase and mitotic Chinese hamster ovary cells and HeLa cells, were modeled as (a) a coated sphere, accounting for nucleus and cytoplasm, and (b) a homogeneous sphere when no cellular nucleus was present. The refractive indices and size distribution of the cells were measured for an accurate comparison of the theoretical model with the light-scattering measurements. The light scattered beyond the forward direction is found to contain information about internal cellular morphology, provided the size distribution of the cells is not too broad.


Photoreactivation and excision repair of ultraviolet radiation-injured DNA in primary embryonic chick cells. Primary embryonic chick cells have been evaluated on the basis of their capacity to repair photochemical lesions produced in the deoxyribonucleic acid (DNA) by ultraviolet (UV) radiation. The fate of one prominent class of UV photoproducts, cyclobutane pyrimidine dimers, was monitored by an in vitro enzymatic assay. UV-irradiated cultures were incubated for prescribed times after which their damaged, radioactive-labeled DNA was extracted and exposed to a purified UV endonuclease selectively active toward sites altered by dimer formation. Single-strand scissions specifically introduced by the enzyme treatment and, therefore, the dimer-containing sites remaining in the DNA were quantified retrospectively by velocity sedimentation in alkaline sucrose. When the chick fibroblasts were incubated in black light, essentially all nuclease-susceptible sites rapidly disappeared from the UV-damaged DNA. In sharp contrast, incubation of the irradiated cultures in total darkness severely impeded the metabolic machinery responsible for site elimination. A substantial amount of UV-stimulated DNA repair synthesis was also detected in the chick cells by conventional techniques involving isopyknic centrifugation and autoradiography. However, the UV photoproducts triggering this indicator of excision repair were probably not dimers since incubation of the irradiated cultures in the light rather than in the dark did not lead to a diminution in the extent of repair synthesis. By these criteria of DNA repair, it appears that embryonic chick cells primarily rely on a highly proficient, light-requiring mechanism, presumably enzymatic photoreactivation, for dimer elimination but also possess a light-independent, excision-type process to cope with other, as yet unidentified, photochemical defects.


Glycogen storage and depletion in human skeletal muscle fibres.


Active and passive cutaneous anaphylaxis in the horse following immunization with benzylpenicilloyl-bovine gamma globulin (BPO20-BGG).


The clinical course associated with dense deposits in the kidney basement membranes.


Linear polypeptides of a known primary structure. Synthesis and immunochemical studies of poly(O-methyl-L-tyrosy-L-glutamyl-L-alanylglycyl)glycine-1-14C ethyl ester.


Insulin degradation by fat cell ghosts.

Insulin degradation by fat cell ghosts.


The adsorption of some proteins on hydroxylapatite and other absorbents used for chromatographic separations.


The translation of mouse globin mRNA from which the polyadenylic acid sequence has been removed in a reinitiating protein synthesis system.


The subunit-structure of the uterine "oestradiol-receptor".


A test of models of membrane structure by molecular hybridization.


Separation of ribonuclease H and RNA directed DNA polymerase (reverse transcriptase) of murine type-C RNA tumor viruses. Ribonuclease H (RNA.DNA-hybrid ribonucleotidohydrolase, EC 3.1.4.34) has been reported to copurify with reverse transcriptase (RNA directed DNA polymerase) of RNA tumor viruses. In addition, viral specific ribonuclease H and reverse transcriptase of avian type-C viruses are thought to be part of the same polypeptide. In this report we show that a fraction of the ribonuclease H activity from Rauscher murine leukemia and Kirsten murine sarcoma viruses was separated from reverse transcriptase by anion exchange chromatography while the remaining portion co-purified with the viral polymerase. The amount of this co-purified nuclease activity was about 4- to 8-fold lower than the activity found in avian myeloblastosis virus (with respect to the ratio of ribonuclease H to reverse transcriptase) and this nuclease activity can only be detected by using labeled substrate of high specific radioactivity. However, a complete separation of ribonuclease H activity from reverse transcriptase was obtained by purifying core structures of the virus by sucrose density gradient centrifugation. While reverse transcriptase was present in the cores, there was no detectable ribonuclease H. Furthermore, a specific antibody against Rauscher leukemia virus reverse transcriptase did not inhibit any virion associated ribonuclease H activity. Our results suggest that in these virions these two enzyme activities reside in two separate molecules and probably in two different compartments of the virus. These findings emphasize a basic difference between the avian and murine type-C virus DNA polymerases.


RNA-dependent DNA polymerase (reverse transcriptase) from avian myeloblastosis virus: a zinc metalloenzyme. RNA tumor viruses contain a characteristic RNA-dependent DNA polymerase (reverse transcriptase) which has been thought to be related to the induction of leukemia by this virus. A disturbance in a zinc-dependent enzyme system was first postulated to account for the demonstrated differences in zinc metabolism of normal and leukemic leukocytes [Vallee et al. in (1949) Acta Unio. Int. Contra Cancrum 6, 869 and (1950) Acta Unio. Int. Contra Cancrum 6, 1102]. In order to investigate the relationship between zinc and the initiation of leukemia in chickens by avian myeloblastosis virus, we have examined the metalloenzyme nature of its reverse transcriptase. The present data show that this protein is a zinc metalloenzyme demonstrating the postulated relationship between zinc and a leukemic process. Paucity of purified enzyme generated the design of a novel system of analysis incorporating microwave-induced emission spectrometry combined with gel exclusion chromatography. It provides precision, reproducibility, and remarkable limits of detection on mul samples containing 10(-12) to 10(-14) g-atoms of metal, and is thus orders of magnitude more sensitive than other methods. The chromatographic fraction with highest enzymatic activity contains 1.8 x 10(-11) g-atoms of zinc per 1.6 mug of protein, corresponding to either 1.8 or 2.0 g-atoms of zinc per mole of enzyme for a molecular weight previously determined either as 1.6 or 1.8 x 10(5). Copper, iron and manganese are absent, i.e., at or below the limits of detection, 10(-13) to 10(-14) g-atoms. Agents known to chelate zinc inhibit the enzyme, while their nonchelating isomers do not. The data underline the participation of zinc in nucleic acid metabolism and bear importantly upon the lesions that accompany leukemia and zinc deficiency.


Craniorachischisis in a partially trisomic 11 fetus in a family with reproductive failure and a reciprocal translocation, t(6p plus;11q minus).


Double autosomal trisomy and mosaicism for chromosomes no. 8 and no. 21.


Presumptive mosaic partial trisomy associated with congenital anomalies and mental deficiency.


Variations in normal human chromosomes.

Variations in normal human chromosomes.


Immunoglobulins.

Immunoglobulins.


Acrocentric associations in mongol populations.


Autosomal deletions 46,XY,del(12)(p11) and 46,XY-46,XY,del(5)(p13) with no effect on physical or mental development.


Chromosome banding patterns in an infant with 13q minus syndrome.


Identification of a 18-21 translocation with Klinefelter's syndrome by G-band patterns.


Investigation of some biochemical genetic markers in four endogamous groups from Panjab (N. W. India). I. Protein and enzyme polymorphisms in serum.


An (11;21) translocation in four generations with chromosome 11 abnormalities in the offspring. A clinical, cytogenetical, and gene marker study.


Evidence for an unlinked gene which modifies HL-A antigen expression.


The penetration of bacteria into experimentally exposed human coronal dentin.


Simple mastectomy for early and advanced mammary carcinoma.


Primary carcinoma of the liver.

Primary carcinoma of the liver.


Humoral immunity in chronic liver disease.


Antibodies to BSA conjugates of morphine derivatives: strict dependency of the immunological specificity on the hapten structure.


Approaches to alcholism in mid-Victorian England.


Anton Störck (1731-1803) and British therapeutics.


Teaching aid: a practical method of demonstrating intensifying screen response.


HL-A antigens and disease. Statistical and genetical considerations.


Beta2-microglobulin on the cell surface. Relationship to HL-A antigens and the mixed leucocyte culture reaction.


1974 National Children's Dental Health Week. Bureau of Dental Health Education.


Colpomicroscopy: basic outlines, technique, application, diagnostics.


Renewal rate of human sebaceous glands.

Renewal rate of human sebaceous glands.


Incidence of circulating antibodies reactive with basal cells of skin in drug reactions.


Chronic mucocutaneous candidiasis, dermatophytosis and defective cellular immunity in monozygotic twins.


Effects of ultraviolet irradiation on human skin lipids. In vivo and in vitro studies.


Histochemical demonstration of thyrotropic and gonadotropic cells in the pituitary gland of the rat by the use of a lead tetraacetate-sodium bisulphite technique.


Localization of free sulphydryl groups by ferricyanide reduction in developing conidiophores of Aspergillus nidulans.


Structure and activity of hepatitis B antigen (HBAg). 1. Studies on some conformational aspects and chemical modification of hepatitis B antigen.


A new variant of Sandhoff's disease.

A new variant of Sandhoff's disease.


Gel chromatography of thyroidal iodo-compounds on sephadex G-10. Separation of iodopeptides, iodine, 3-monoiodotyrosine, 3,5-diiodotyrosine and iodothyronines.


Acid soluble nuclear proteins of rat liver: differential absorbance of bound dyes and changes in neoplasia.


Simultaneous determination of particulate and molecular lead in the atmosphere.


Proceedings: Influence of histamine releasing agents on tetragastrin-stimulated acid secretion of isolated bullfrog gastric mucosa.


Gangliosidosis (GM1). A re-evaluation of the vertebral deformity.


Cell-mediated immunity to Friend virus-induced leukemia.


Manipulating the patient. A comparison of the effectiveness of physician and chiropractor care.


Letter: Diabetic ketoacidosis and intracerebral thrombosis.


Letter: G.A.B.A. in Huntington's chorea.


Letter: Propranolol and thyroidectomy.

Letter: Propranolol and thyroidectomy.


Plasma cell leukemia. Unassembled light and heavy chains in the urine.


Editorial: Universities and medical schools.


Increased urinary albumin-excretion rate in benign essential hypertension.


Editorial: Pathologists at the crystal ball.


Editorial: Defibrination with head injury.


The lymphocyte in chronic lymphatic leukaemia.


Geriatric psychiatry in the general hospital.


Epidemic of postoperative wound infection associated with ungloved abdominal palpation.


Letter: Tuberculosis retreats--slowly.

Letter: Tuberculosis retreats--slowly.


Letter: Possible evidence for Xp plus in and XX Male.


Letter: Neonatal deaths and sex-chromosome anomalies.


Letter: Chloramphenicol.

Letter: Chloramphenicol.


Letter: Suprastriate hemianopia.

Letter: Suprastriate hemianopia.


Letter: Effect of prostaglandin E2 therapy on the cerebral cortex.


Letter: Prostaglandins and induction of labour. Professor Brosens and others (April 27, p.808) suggested that the use of prostaglandins (PGs) for induction of labor in pregnancies complicated by hypertensive states might result in a high incidence of fetal distress.  Our program of PG clinical investigation has, over the past 3 years, generated case records of 1044 women whose labor was induced with either PGF2alpha or PGE2.  Among these records are reports of 283 cases in which the reason for inducing labor was either preeclamptic toxemia or hypertension.  The mean Apgar scores at 1 and 5 minutes for the infants in this group were 8.15 and 9.42 respectively.  The respective modes were 9 and 10.  Of the 4 infants with an Apgar under 5 at 5 minutes, 2 had midcavity forceps deliveries for fetal distress in the 2nd stage.  All infants were followed up for at least 1 week after delivery and, at that time, all were normal.  It is submitted that these figures do not suggest an abnormal incidence of fetal distress.  With regard to the animal studies which Brosens and his colleagues suggested, the article by Clark et al. is of interest.  These workers infused PGF2alpha into uterine arteries of pregnant dogs in doses of between 1 and 100 mcg/minute, and were unable to demonstrate any alteration in vascular tone.  It should be remembered that the usual recommended infusion rate of PGF2alpha for induction of labor in humans is 5 mcg/minute.  This suggests that PGs used at the recommended doses for induction of labor are unlikely to compromise the uteroplacental bloodflow.  The predictive value in humans of the animal studies suggested is worth considering since marked species differences are well known in the case of PGs.  Of importance, however, must be the substantial body of clinical evidence now available on the safety of PGE2 and PGF2alpha when used for induction of labor in those with and without hypertension.  The absence of an antidiuretic effect and the established efficacy of PGs in inducing labor preterm suggest that they may become the treatment of choice in these circumstances.


Letter: A.H.H. inducibility and lung cancer.


Duration of treatment of phenylketonuria.


Letter: Hospital service for children with infective diseases.


Letter: Complement components in cystic fibrosis.


Letter: Has anyone seen Betty?

Letter: Has anyone seen Betty?


Letter: Glucagon in treatment of acute pancreatitis.


Letter: S.I. units.

Letter: S.I. units.


Letter: Judging clinical competence.

Letter: Judging clinical competence.


Letter: Cellular immune responsiveness in cigarette smokers.


Letter: A.F.P. and ataxia-telangiectasia.


Letter: Geriatrics is medicine.

Letter: Geriatrics is medicine.


Letter: Oral contraceptives and vitamin nutrition.


Letter: Burkitt's lymphoma.

Letter: Burkitt's lymphoma.


Letter: Seasonal variations in serum 25-hydroxycholecalciferol.


Letter: Birth defects and oral hormone preparations. A specific malformation syndrome given the acronym VACTEL (Vertebral, anal, cardiac, tracheal, esophageal, and limb defects) had been associated with exogenous hormones taken by the mother in early pregnancy.  A study of hormonal pregnancy tests and their possible relationship to congenital malformations suggested a high frequency of use of hormonal pregnancy tests in cases of esophageal atresia.  The authors conducted a retrospective study of patients with esophageal atresia born in the southwest of England from 1942 to 1973, including both live and stillbirths.  Data on sales of hormonal pregnancy test tablets were provided by manufacturers of the test tablets and by Intercontinental Medical Statistics Limited.  Of 345 cases of esophageal atresia studied, no single case of VACTEL association was observed, although 55% of the cases had malformations.  The study did not support the suggestion that oral contraceptives or oral hormone pregnancy test tablets cause esophageal atresia alone or as part of the VACTEL association.


Letter: Unusual intranuclear filaments in multiple-sclerosis brain.


Letter: Prolactin and hypercalcaemia.

Letter: Prolactin and hypercalcaemia.


Proceedings: Long-term radiological assessment of juvenile rheumatoid arthritis.


Terminal deoxynucleotidyltransferase in murine plasmocytomas.


A glucagonoma syndrome.

A glucagonoma syndrome.


Treatment of uraemic pericarditis by anterior pericardiectomy.


Assessment of response to treatment in advanced breast cancer. British Breast Group.


Letter: B lymphocytes in inflammatory bowel disease.


Letter: Lymphocyte response after radiotherapy.


Letter: Propranolol in migraine.

Letter: Propranolol in migraine.


Simple rheumatoid agglutination test with non-diluted serum and blood.


Proceedings: Stimulation of penicillin-sensitive lymphocytes by antigenic determinants covalently bound to cell membranes.


Polymers as surgical implants.

Polymers as surgical implants.


Identification of cells in primate bone marrow resembling the hemopoietic stem cell in the mouse.


Histochemical staining patterns of muscle fibres in the gastrocnemius, soleus and anterior tibial muscles of the adult cat, as viewed in serial sections stained for lipids and succinic dehydrogenase.


Irradiation of epidermal cell suspensions in the presence and absence of protoporphyrin.


Adrenal corticosteroids enhance production of type-C virus induced by 5-iodo-2'-deoxyuridine from cultured mouse fibroblasts. Induction of RNA "tumor" viruses by 5-iodo-2'-deoxyuridine in mouse fibroblasts is stimulated 5- to 25-fold by glucogenic adrenal corticosteroids. Enhancement of virus production by the hormones is inhibited by low concentration of cordycepin, an inhibitor of poly(A) synthesis.


A negative staining-carbon film technique for studying viruses in the electron microscope. I. Preparative procedures for examining icosahedral and filamentous viruses.


Trophic influence of nerves on the development and maintenance of sensory receptors.


Con A differentiates among grass pollens by binding specifically to wall glycoproteins and carbohydrates.


Autogenous vein grafts in experimental canine atherosclerosis. Their fate in the abdominal aorta and peripheral arteries.


Immunological cross-reactions of sulfobenzyylpenicillin.


Differential inhibition of transcriptions catalyzed by T7-specific and Escherichia coli RNA polymerases by bleomycin.


Interactions of DNA with zorbamycin, phleomycin and bleomycin; ultraviolet absorption and circular dichroism measurements.


The effect of bleomycin on SV40 DNA: characteristics of bleomycin action which produces a single scission in a superhelical form of SV40 DNA.


Effects of bleomycin on PHA-stimulated lymphocytes and isolated hepatic nuclei.


Baboon virus isolate M-7 with properties similar to feline virus RD-114. A virus (M-7) isolated from baboon placental tissue demonstrates many similarities to endogenous feline virus RD-114. Immunodiffusion analysis shows a group-specific antigen (gs-1) line of identity between M-7 and RD-114. Anti-RD-114 DNA polymerase IgG inhibits M-7 polymerase by 57% compared to 97% for RD-114. M-7 virus has helper activity as demonstrated by rescue of murine sarcoma virus (MSV) from sarcoma-positive leukemia-negative human amnion cells. The host range of the rescued M-7 pseudotype of MSV, MSV (M-7), is similar to that of RD-114 virus. MSV (M-7) is also able to transform baboon cells and causes no detectable transformation of feline cells without addition of helper feline leukemia virus. Interference properties of M-7 and RD-114 virus are identical. Virus-specific neutralizing antisera, although partially cross-reacting, can distinguish MSV (M-7) from MSV (RD-114). These similarities and differences between RD-114 and M-7 viruses are best explained as type-specific differences between two viruses within the same strain.


A method for detecting Ig rheumatoid factors in seronegative rheumatoid arthritis.


Mastocytes and some biologically active compounds in experimental trichinellosis.


Pathomorphology and serum protein electrophoresis in animals intensively stimulated with thphoid fever vaccine.


Comparison of morphological and physico-chemical properties of flagella and flagellins of Proteus mirabilis and its R mutant.


Axoplasmic transport.

Axoplasmic transport.


Amine-producing endocrine-like cells in the epithelium of urethra and prostate of the guinea-pig. A chemical, fluorescence histochemical, and electron microscopic study.


Immunological studies of membrane glycoproteins isolated from human breast carcinomas.


Functional black adenoma of the adrenal cortex. A rare cause of primary aldosteronism.


Metachromatic staining of basic nucleoproteins in chronic erythremic myelosis.


The chicken A-1 protein. Phylogenetic variation in the amino acid sequence of the encephalitogenic site.


Structural studies on chick embryonic hemoglobins.


Ultrastructure of 90Sr induced osteosarcomas and early phases of their development.


To the memory of professor A. Biemond.

To the memory of professor A. Biemond.


Vaccinia antibodies in cerebrospinal fluid of patients with multiple sclerosis.


The prophylactic and therapeutic uses of immunoglobulin in virus infections.


Encapsulation of lymphocyte DNA by vesicular stomatitis virus. Vesicular stomatitis virus, grown on the WIL(2)-3A line of continuously growing human lymphocytes, contains DNA in addition to the viral RNA. By contrast, virus grown on BHK 21-C13 fibroblasts has no detectable DNA. The virus-associated DNA is found in both the B and T particles of the virus and is resistant to deoxyribonuclease. The DNA is intimately associated with the virus and appears to be incorporated into the viral ribonucleoprotein core structure produced by treatment of the virus with Nonidet P40 follow edby CsCl isopycnic banding. The virus-associated DNA has an isopycnic density of 1.699 g x cm(-) (-3) in CsCl, identical to that of human DNA. The average molecular weight of the DNA molecules associated with the virus is 9.0 x 10(5), as determined by velocity sedimentation in sucrose density gradients and studies of its contour length in the electron microscope. DNA.DNA reassociation kinetics of this DNA demonstrate that the DNA is of host origin and rules out the possibility that it originates from contaminating microorganisms or mitochondria. The analytical complexity of the virus-associated DNA shows that 50% of the virus-associated DNA sequences are homologous to the repeated DNA sequences of human DNA and the remaining 50% are homologous to human unique sequences. On the average, there is one molecule of DNA for each four virion particles.


NS-1 (nervous system antigen-1), a glial-cell-specific antigenic component of the surface membrane. A methylcholanthrene-induced glioblastoma of the C57BL/6 inbred mouse strain was used to raise antibodies in C57BL/6 and C57BL/10 inbred mice and in (C57BL/6 x DBA/2) and (C57BL/6 x Balb/c) F(1) hybrids. When examined by the cytotoxicity test, these antibodies define a cell-surface component (or components) found exclusively on brain tissue of all mouse strains studied and of several other mammalian species including man. The antigen, named NS-1 (nervous system antigen-1), is present on cells of three of the four mouse-glial-cell tumors tested, but not on the C1300 neuroblastoma, a tumor of neuronal origin. NS-1 occurs in higher concentration in regions of the nervous system richer in white than in gray matter, and in lower than normal concentrations in brains of myelindeficient neurological mutant mice. The concentration of NS-1 gradually increases postnatally and reaches the adult level between the third and fourth week. The existence of more than one allele or genetic locus controlling NS-1 activity is suggested by the occurrence of higher amounts of NS-1 in brains of the A and C57BL/6 than of the Balb/c and DBA/2 mouse strains. NS-1 is the first cellsurface component to be described that is not only unique to nervous tissue, but specific for glial cells.


Intracellular localization of gastrin in the canine antral mucosa by the immunoenzyme technique.


Immunological identity of the small subunit of HL-A antigens and beta2-microglobulin and its turnover on the cell membrane. A number of immunological methods have been employed to show that the small subunit of HL-A antigens, isolated either after papain treatment or after solubilization with detergent, is identical to beta(2)-microglobulin, a protein previously isolated from human urine and shown to be homologous in structure to constant region domains of immunoglobulins. Moreover, quantitative data indicate virtually total identity between the small subunit of HL-A antigens and beta(2)-microglobulin. Studies of the turnover of labeled HL-A antigens from the lymphocyte surface indicate that the two subunits turn over at similar rates, although only the small subunit could be detected in the culture medium. The significance of these observations is discussed.


Tubocurarine requirements and plasma protein concentrations in the newborn infant.


Sjögren's syndrome in progressive systemic sclerosis (scleroderma).


The distribution of x-ray induced chromosomal abnormalities in rat kangaroo (Potorous tridactylis) cells in vitro.


Axonal transport of newly synthesized glycoproteins in a single identified neuron of Aplysia californica. Increasing amounts of glycoprotein synthesized from L-[(3)H]fucose injected into the cell body of R2, an identified Aplysia neuron, were found in the right pleuro-abdominal connective. Autoradiography revealed that the glycoproteins were localized in the axon of R2. Glycoproteins appearing in the axon presumably were synthesized in the cell body, since no significant incorporation was observed when [(3)H]fucose was injected directly into the axon. [(3)H]glycoproteins were detected in the connective after a delay of 1 h after intrasomatic injection. Thereafter, transport from the cell body was rapid, and by 10 h after injection, 45% of the total neuronal [(3)H]glycoprotein had appeared in the axon. By analysing the radioactivity in cell body and connective 4, 10, and 15 h after injection, we found that [(3)H]glycoproteins were transported selectively compared to nonmacromolecular material. Sequential sectioning of the connective revealed that [(3)H]glycoproteins were transported in discrete waves. The population of membrane-associated [(3)H]glycoproteins in the axon differed from that in the cell body. Two of the five somatic components appeared to be transported preferentially. In addition a new component appeared in the axon 10 h after injection.


Effects of Ca2+ and Mg2+ on rapid axonal transport of proteins in vitro in frog sciatic nerves.


Immediate hypersensitivity and periodontal disease.


Effects of serum lipoproteins on the morphology, growth, and metabolism of arterial smooth muscle cells.


Patient care, education, and research in pathology.


Affinities of progestogen and estrogen receptors in rabbit uterus for synthetic progestogens.


PAS-bisulfite stain for diabetic angiopathy.


Separation of single-stranded ribonucleic acids by acrylamide-agarose-urea gel electrophoresis.


Histochemical and electron microscopic observations on the development, neural control and function of the Paneth cells of the mouse.


Focal and segmental sclerosing glomerulopathy (-nephritis).


Induction of ethylmorphine demethylation and aniline p-hydroxylation by various catatoxic steroids in regenerating rat liver.


Immunochemical and enzymic properties of the electric eel acetylcholinesterase-antiacetylcholinesterase system.


X-XY mosaicism with short y.

X-XY mosaicism with short y.


Muscle histology in fetuses at risk for Duchenne muscular dystrophy.


Selective roles of thymus-derived lymphocytes in the antibody response. I. Differential suppressive effect of carrier-primed T cells on hapten-specific IgM and IgG antibody responses. Passively transferred thymocytes and spleen cells from donors primed with keyhole limpet hemocyanin (KLH) exerted differential suppressive effect on IgM and IgG antibody responses of syngeneic recipients immunized with DNP-KLH depending primarily on the time when KLH-primed cells were transferred. This was demonstrated by the decrease in the numbers of DNP-specific direct and indirect PFC in the spleen of the recipients given KLH-primed cells at different times during primary and secondary immunization. Whereas the cell transfer simultaneously with or 2 days after the primary immunization produced only slight suppression of the peak IgM antibody response, it caused profound suppression of late IgM and IgG antibody responses. By contrast, the cell transfer 3 days after the immunization produced immediate suppression of the ongoing IgM antibody response resulting in its earlier termination, while being unable to prevent the induction of IgG antibody response. KLH-primed cells could moderately suppress the secondary anti-DNP antibody response, in which IgG antibody response was found to be slightly more sensitive than IgM antibody response to the suppressive influence of KLH-primed cells. The suppressive effect of the KLH-primed spleen cells was completely eliminated by the in vitro treatment of the cells with anti-theta and C before cell transfer, indicating that cells responsible for the suppression are, in fact, T cells. The suppression of DNP-specific antibody response by KLH-primed T cells was achieved only if the recipients were immunized with DNP-KLH but not with DNP-heterologous carrier, suggesting that direct interaction between T and B cells is necessary for the suppression of the antibody response. It is concluded that susceptibility of B cells to the specific suppressive influence of T cells is inherently different depending on the differentiation stage of B cells and on the immunoglobulin class they are destined to produce.


Influence of a combined oral contraceptive upon rats fed low protein-high carbohydrate diets.


Primary and secondary cerebrospinal fluid acidosis as a manifestation of cerebral lesions.


Pattern of luteinizing hormone release in progestin-treated ewes.


Luteinizing hormone release and ovulation in anestrous ewes.


Virological investigations of Herpesvirus saimiri-infected owl monkeys.


Value of increased ESR in cold as a test of autoimmunity and its comparison with standard tests.


Electron microscopical and cultural features of Neisseria meningitidis competence variants.


Purification and characterization of serologically active cell wall substances from Planococcus strains.


Invited review. Thalassemia: an abnormality in globin chain synthesis.


Retinoblastoma with necrosis of central retinal artery and vein and partial spontaneous regression.


Experimental infection of Strongyloides westeri in parasite-free ponies.


Morphology of mesothelial cells in peritoneal fluid from normal women.


S-adenosylmethionine: protein-carboxyl methyltransferase from erythrocyte.


Identification of graft and host cells in bone marrow transplants by the quinacrine banding technique of chromosome identification.


Myeloma producing nonsecretory IgM and secretory IgG.


Adenoid cystic carcinoma of the trachea. Experience with 16 patients managed by tracheal resection.


An exclusive right thoracic approach for cancer of the middle third of the esophagus.


Letter: Cerebrospinal-fluid electrophoresis in SSPE.


The preferential invasion of young erythrocytes in acute Babesia bigemina infections of splenectomised calves.


Immunology of dermatophytes and dermatophytosis.


The fine structure of the microconidium of Blastomyces dermatitidis.


Middle ear disease in children. Pathogenesis, diagnosis, and management.


An unusual histological cataract.

An unusual histological cataract.


Canine ceroid-lipofuscinosis-a model for human neuronal ceroid-lipofuscinosis and aging.


Chemical pathology of multiple sclerosis.


Studies on pathogenesis of cervical spondylotic myelopathy: sagittal diameter of the spinal canal and cerebrospinal fluid characteristics in cervical spondylosis.


Common antigenic structures of HL-A antigens. V. An antigenic determinant characteristic of a 33,000-dalton fragment of HL-A molecules.


HL-A antigens on circulating platelets. Ultrastructural demonstration.


Hepatic lipid in Reye's syndrome: histochemical and ultrastructural characteristics.


Human dermatophilosis, its occurrence in Brazil.


Interpretation of synovial fluid findings in the horse.


Cytochemistry of acid mucosubstance and acid phosphatase in Cryptococcus neoformans.


The negative staining of "difficult" bacteria like Arthrobacter globiformis for electron microscopy.


Polarization of the 515 nm effect on chloroplasts oriented by a magnetic field.


Biochemical studies on gramicidin S non-producing mutants of Bacillus brevis ATCC 9999.


Triiodothyronine and thyroxine in hyperthyroidism. Comparison of the acute changes during therapy with antithyroid agents. In 66 untreated patients with hyperthyroidism, serum triiodothyronine (T(3)) and thyroxine (T(4)) concentrations were measured by immunoassay. The mean T(3) level was 478+/-28 ng/100 ml (all values mean+/-SEM) and the T(4) was 20.6+/-0.6 mug/100 ml. The serum T(4)/T(3) ratio by weight was 48+/-2 as opposed to a value of 71+/-3 in euthyroid adults. There was a significant inverse correlation of the T(4)/T(3) ratios with serum T(3) (r=0.77; P<0.01) but not with serum T(4)(r=0.21). These results suggested that relative overproduction of T(3) is consistently present in patients with hyperthyroidism. To examine the acute effects of various antithyroid agents on serum T(3) and T(4) concentrations, iodide, propylthiouracil (PTU), and methylmercaptoimidazole (MMI) were given alone to mine patients, and serial T(3) and T(4) measurements were made. There was an acute decrease in serum T(3) over the first 5 days in the three iodide and three PTU-treated patients which was greater than that seen in the MMI group. This suggested that PTU and MMI had different effects on T(3) production. To compare the effects of PTU and MMI under conditions in which thyroidal hormone release was minimized, these drugs were given in combination with iodide. The mean daily dosage of PTU was 827 (n=11) and of MMI was 88 (n=8). In the PTU+iodide group, the initial serum T(3) concentration was 586+/-61 ng/100 ml and decreased significantly to 326+/-41 on day 1 and to 248+/-21 on days 2 and 3, respectively, and did not change further on days 4 and 5. In the MMI + iodide group, basal serum T(3) was 645+/-90 ng/100 ml and decreased to 568+/-81, 452+/-73, and 344+/-51 on days 1, 2, and 3, respectively, and did not change thereafter. While the initial T(3) concentrations in serum were not different in the PTU and MMI groups, the T(3) concentrations in the PTU patients were significantly lower on days 1 and 2 and during the apparent plateau period on days 3-5. Serum T(4) concentrations decreased gradually in both groups, from 23.9+/-2.0 mug/100 ml, initially, to 17.5+/-1.6 on day 5 in the PTU group and from 22.0+/-2.6 to 14.6+/-2.0 in the MMI-treated patients. The T(4) values were not significantly different at any time. These changes resulted in increases in the serum T(4)/T(3) ratios in both groups, but these ratios were substantially higher in the patients treated with PTU + iodide. The initial serum T(4)/T(3) ratio was 43+/-3 and increased to 74+/-7 and 88+/-7 on days 1 and 2 in the PTU group, reaching a plateau value of 91+/-7 during days 3-5. Comparable values for MMI-treated patients were 35+/-2, 42+/-3, 52+/-6, and 54+/-3 during the plateau period. Previous investigations have shown that PTU inhibits T(4) deiodination in hyperthyroid patients and decreases T(3) production from T(4) in animals. The greater acute decrease in serum T(3) and the higher serum T(4)/T(3) ratios in the PTU-treated patients seems best explained by an inhibition of peripheral T(3) production by this agent. This conclusion is further supported by a direct relationship between the T(4)/T(3) ratio on days 3-5 and the dose of PTU administered. These results further suggest that both thyroidal and extrathyroidal pathways contribute substantially to the apparent overproduction of T(3) in hyperthyroidism.


Prolonged survival in a patient with hepatic malignant melanoma treated by intra-arterial bleomycin and oral hydroxyurea.


An analysis of the break points of structural rearrangements in man.


Partial trisomy 11 in a child resulting from a complex maternal rearrangement of chromosomes 11, 12 and 13.


Cross-reactions between human and animal plasma proteins. V. The role of the antiserum and immunological specificity in relation to the phenomenon of cross-reaction.


Unusual chromosome bands revealed by aging.


Frequency and genetic effect of 1qh plus.


Beta-2-microglobulin. Part of the HL-A molecule in the cell membrane.


The relation between the "Alte Wiener Schule" and the Spanish medicine of the Enlightenment.


Michael Scot and Mary of Bologna: an addendum.


Accessory sex glands and their secretions in male Periplaneta americana (L.). II. Secretory behaviour and maturation of the mushroom gland complex.


Gynecomastia in association with administration of thiacetazone in the treatment of tuberculosis.


Serum alpha fetoprotein in childhood.

Serum alpha fetoprotein in childhood.


Differentiation between early and late membrane antigen on human lymphoblastoid cell lines infected with Epstein-Barr virus. II. Immunoelectron microscopy.


Biochemical and morphologic evidence for the presence of an RNA tumor virus in pulmonary carcinoma of sheep (Jaagsiekte).


Argentaffin and argyrophil cells in the prostate.


Hydralazine-induced Lupoid syndrome. Biochemical and immunological studies.


Long-term exposure to auto exhaust and other pollutant mixtures: effects on pulmonary function in the beagle.


Natural history of Ebstein's anomaly of tricuspid valve in childhood and adolescence. An international co-operative study of 505 cases.


Pseudo AV block produced by concealed extrasystoles arising below the bifurcation of the His bundle.


Maintenance of human tumours and tissues in immunosuppressed mice.


Elastic tissue in uterine blood vessels.


Inhibitory effect of foetal alpha 2 globulin, an acute phase protein, on carrageenin oedema in the rat.


Differential spiralization along mammalian mitotic chromosomes. II. 5-bromodeoxyuridine and 5-bromodeoxycytidine-revealed differentiation in human chromosomes.


Center for Barr body condensation on the proximal part of the human Xq: a hypothesis.


Localization of rDNA in the chromosome complement of the rhesus (Macaca mulatta).


Variable satellite DNA's in the African green monkey Cercopithecus aethiops.


B-chromosomes in the myrmicine ant, Leptothorax spinosior.


Descending connections from the mesencephalon to the inferior olive: an experimental study in the cat.


Demonstration by degeneration silver method of the ascending projection from the locus ceruleus.


Immunological heterogeneity of Clostridium botulinum type B toxins.


Studies on the lethal action of toxotoxin.


Unscramble your word power: learning aid for medical terminology.


The medium is as important as the message.


Endogenous rat C-type virus in transplantable rat tumors.


Fine structural topochemistry of entamoeba histolytica Schaudinn, 1903.


dsDNA made by RNase-sensitive DNA polymerase from RSV-transformed cells.


Surface H-2 antigen concentration requirement of somatic hybrid cells for IgM-mediated cytotoxicity.


Subunit structure of HL-A antigens on cell surface.


Identification of chromosomal abnormalities by quinacrine-staining technique in patients with normal karyotypes by conventional analysis.


Letter: Nonketotic hyperosmolar diabetic coma in an infant.


Metabolism of albumin and body fluid compartments in protein deficiency--an experimental study in the rhesus monkey.


Letter: Gammaglobulin and congenital rubella.


Some ultrastructural observations of haematoxyphil vascular change in oat-cell carcinoma.


Regional approach to poison control.

Regional approach to poison control.


The cytochemical bioassay of corticotrophin.


Differential DNA synthesis in homologous regions of hybrid polytenic chromosomes (Anopheles atroparvus x A. labranchiae).


A phylogenetic study of bird karyotypes.


Blood groups of chimpanzees.

Blood groups of chimpanzees.


Letter: Scabies in babies.

Letter: Scabies in babies.


Nitrogenase.

Nitrogenase.


Banding and chromatid separation in Chinese hamster chromosomes.


Letter: Spotted centromeres in human chromosomes.


Hyperthyroidism associated with pregnancy.


A staining method for keratohyalin granules.


Proceedings: Search for a viral etiology of human breast cancer.


The sialic acid content in mouse female reproductive organs as a quantitative parameter for testing the estrogenic and antiestrogenic effect, antiestrogenic depot effect, and dissociated effect of estrogens on the uterus and vagina.


Primary lipoidal degeneration of the cornea.


Choriocarcinoma of the testis.

Choriocarcinoma of the testis.


Pulmonary fat embolism. An ultrastructural study.


Argyrophil cells and melanocytes in esophageal mucosa.


Muscle regeneration in the dystrophic Syrian hamster tongue. Effects of a moderately severe crushing injury.


RNA directed DNA polymerase in C-type particles form normal rat thymus cultures and Moloney leukemia virus.


Induction of C-type particles in mammalian cancer cells.


Letter: Angio-immunoblastic lymphadenopathy with dysproteinaemia.


Inequality and the health service.

Inequality and the health service.


Clinical evaluation of the turkey-erythrocyte passive-haemagglutination test for hepatitis-B surface antigen.


Drug-associated diarrhoea as a potential reservoir for hospital infections.


Editorial: Tobacco smoke and the non-smoker.


Psychiatric illness in doctors.

Psychiatric illness in doctors.


Letter: Typhoid fever treated with chloramphenicol and co-trimoxazole.


Letter: Double failure of technically correct laparoscopic sterilization.


Letter: A new leg-prosthesis socket.

Letter: A new leg-prosthesis socket.


Letter: Intravenous metronidazole in amoebic liver abscess.


Letter: Post-pill fertility.

Letter: Post-pill fertility.


Letter: Ankylosing spondylitis, sacroiliitis, regional enteritis, and HL-A 27.


Tentative method of analysis for polynuclear aromatic hydrocarbons in automobile exhaust.


Morphology of bacteriophage-like particles from Fusobacterium symbiosum. The morphology of bacteriophage-like particles from the strict anaerobe Fusobacterium symbiosum is described. Attempts to demonstrate plaque formation on the host strain of F. symbiosum and other related species were unsuccessful.


Beat-by-beat stroke volume from aortic-pulse-pressure analysis.


A simple method for R banding of human chromosomes, showing a pH-dependent connection between R and G bands.


Bacterial plaque disclosure survey.

Bacterial plaque disclosure survey.


Salbutamol by intermittent positive pressure ventilation in status asthmaticus.


Studies on the cephalochordates of the Madras coast. 15. The nature of the structural polysaccharide in amphioxus, Branchiostoma lanceolatum.


Cytochemical study of non-specific esterase, acid and alkaline phosphatase in the human thyroid neoplasms.


Histochemical analysis of thermal effects on the high pH metachromasia of galactogen.


Histochemical study on the oxidative enzymes of the interstitial cells of the renal medulla in rats.


Basic nuclear proteins in a coelenterate; a cytochemical demonstration of histones in Hydra.


Some enzyme histochemical studies of the transitional epithelium of mammalian urinary tract.


Histochemical aspects of hydatidiform mole and choriocarcinoma.


Chemical nature of the hooks of the cystacanth of Moniliformis moniliformis.


Are patients satisfied?

Are patients satisfied?


Regulation of hepatic transketolase activity by thiamin.


Dental school application is a disgrace.


Evaluation of a prospective dental student.


On becoming a professional.

On becoming a professional.


What is a professional?

What is a professional?


Is prolactin involved in precipitating migraine?


Brain-decarboxylase activities as indices of pathological change in senile dementia.


Is an acute disturbance in hepatic transport of bile-acids the primary cause of cholestasis in benign recurrent intrahepatic cholestasis?


Spread of meningococcal infection within households.


Letter: Nephritis after irradiation and chemotherapy for nephroblastoma.


Letter: Effect of baclofen on myotonia.

Letter: Effect of baclofen on myotonia.


Recurrent meningococcal infection with an antigenically identical strain.


Humoral response in patients with liver disease.


Detection of protein subunits of ferritin in situ in cells by immunofluorescence. Antisera raised in rabbits against protein subunits of rat liver ferritin contain antibodies (IgG) that precipitate subunits and other antibodies (IgG) that precipitate undissociated ferritin and apoferritin. Removal of the latter antibodies from IgG fractions of immune sera by repeated addition of ferritin leaves antibodies that specifically precipitate subunits. Using these subunit-specific antibodies, we have demonstrated the presence of subunit-positive sites in cells by immunofluorescence. Subunit-positive sites were best demonstrated in rats that had been loaded with iron. These sites were diffusely spread through the cytoplasm of hepatocytes and Kupffer cells in rat livers and of macrophages and fibroblasts in rat livers and hearts. With antibodies to ferritin, the presence of ferritin was demonstrated in the same kinds of cells. Although, by immunofluorescence, the intracellular localization of ferritin-positive and subunit-positive sites was similar, ferritin-positive immunofluorescence was most intense in Prussian blue-positive cytoplasmic granules in which high concentrations of ferritin were presumed to be present on the basis of electron microscopic studies. Our findings can be interpreted to indicate either the presence of unassociated subunits or of fragments of subunits in the cytoplasm of the several kinds of cells studied. It is also possible that partly assembled molecules of ferritin or apoferritin in these cells reacted with subunit-specific antibodies. Subunit-specific antibodies may be useful for localizing sites of assembly of ferritin molecules at the level of cell fine structure and for elucidation of the biosynthesis of ferritin.


Modified two-dimensional polyacrylamide gel electrophoresis systems for higher molecular weight nonhistone chromatin proteins from normal rat liver and Novikoff hepatoma ascites cells.


Loss of DNA following C-banding procedures.


Localization of sister chromatid exchanges in human chromosomes. The bromodeoxyuridine; sensitivity of 33258 Hoechst fluorescence allows microfluorometric analysis of sister chromatid exchanges in human metaphase chromosomes. The frequency of sister chromatid exchanges among chromosomes correlates with chromosome length. Exchanges appear to occur predominantly in interband regions, as defined by quinacrine fluorescence, or very near band-interband junctions. A few regions are involved unusually frequently.


Letter: Lactic acidosis and hyperlactataemia.


Editorial:  Screening for hepatitis B.

Editorial:  Screening for hepatitis B.


Directionality of brain microtubule assembly in vitro. Chicks were injected intra-cerebrally with [(3)H]leucine, and (3)H-labeled microtubules were assembled in vitro in the brain supernatants. Pieces of these (3)H-labeled microtubules were then incubated with unlabeled brain tubulin subunits under conditions where the subunits assembled onto the labeled pieces. Electron microscopic autoradiography of the negatively-stained microtubules showed all of the radioactivity at one end of the tubules as they increased in length. This clearly demonstrated that the microtubules of brain in this in vitro system were being assembled unidirectionally.


Regulatory processes of DNA synthesis in the embryo.


Detection of areas of wall differentiation in fungi using fluorescent staining.


Response in macromolecular syntheses of mouse L cells to bleomycin, with special reference to cell-antibiotic interaction.


Measurements of mammalian cellular DNA and its localization in chromosomes.


Isolation of mammalian heterochromatin and euchromatin.


Quantitative light microscopic autoradiography.


Effect of bleomycin on the chromosomes of human lymphocytes at various cell phases.


Enhancing effect of clamping of the portal vein on the effectiveness of antitumor agents against abdominal tumor growth.


Methods in the cellular and molecular biology of paramecium.


Isolation of hepatocytic mitosis-inhibiting protein from normal rat plasma.


Sex-hormone-binding globulin.

Sex-hormone-binding globulin. A review was made to understand how plasma binding protein might influence sex-hormone action in target tissues.  Steroids are predominately bound to plasma proteins and only unbound steroids enter the cells.  Sex-hormone-binding globulin (SHBG) binds to both the main circulating steroid T and E2 but changes in SHBG concentrations exert significant results.  Increased SHBG levels increase estrogen production and decreases T activity; whereas, increased androgens increase T action and inhibit SHBG production.  These disturbances in hormone maintenance may lead to abnormal adult sex differentiation such as hirsutism and forms of hynaecomastia.  By developing SHBG concentration measurement methods-responses of hirsutism to glucocorticoid or estrogem may be assessed.  In addition, the effect of thyroid hormones on SHBG may also have therapeutic implications in endocrine disease.


Cyclical phenomena in persistent virus infection.


Interaction of ruthenium red with isolated sarcolemma.


Long-term serial cell cultivation of human brain tumor in vitro.


Relationship of uptake of sodium and 45calcium to ATP-induced histamine release from rat mast cells.


Aetiology of multiple sclerosis.

Aetiology of multiple sclerosis.


Demonstration of a fibrillar component in the cell wall of the yeast Saccharomyces cerevisiae and its chemical nature. The ultrastructure of isolated cell walls of Saccharomyces cerevisiae from the log and stationary phases of growth was studied after treatment with the following enzymes: purified endo-beta-(1 --> 3)-glucanase and endo-beta-(1 --> 6)-glucanase produced by Bacillus circulans; purified exo-beta-glucanase and endo-beta-(1 --> 3)-glucanase produced by Schizosaccharomyces versatilis; commercial Pronase. While exo-beta-glucanase from S. versatilis had no electron microscopically detectable effect on the walls, Pronase removed part of the external amorphous wall material disclosing an amorphous wall layer in which fibrils were indistinctly visible. Amorphous wall material was completely removed by the effect of either endo-beta-(1 --> 3)- or endo-beta-(1 --> 6)-glucanase of B. circulans or by a mixture of the two enzymes. As a result of these treatments a continuous fibrillar component appeared, composed of densely interwoven microfibrils resisting further action by both of the B. circulans enzymes. The fibrillar wall component was also demonstrated in untreated cell walls by electron microscopy after negative staining. Because of the complete disappearance of the fibrils following treatment with the S. versatilis endo-beta-(1 --> 3)-glucanase it can be concluded that this fibrillar component is composed of beta-(1 --> 3)-linked glucan. Bud scars were the only wall structures resistant to the effect of the latter enzyme.


Prophasing of interphase nuclei and induction of nuclear envelopes around metaphase chromosomes in HeLa and Chinese hamster homo- and heterokaryons. Fusing human HeLa metaphase cells with HeLa interphase cells resulted within 30 min in either of two phenomena in the resultant binucleate cell: either prophasing of the interphase nucleus or formation of a normal-appearing nuclear envelope around the metaphase chromosomes. The frequency of either occurrence was strongly dependent on environmental pH. At pH's of 6.6-8.0, prophasing predominated; at pH 8.5 nuclear envelope formation predominated. Additionally, the frequencies of the two events in multinucleate cells depended on the metaphase/interphase ratio. When the ratio was 0.33 nuclear envelope formation predominated; when it was 2.0 prophasing predominated. In their general features, the results with fused HeLa cells resembled those reported earlier with fused Chinese hamster Don cells. However, the results provided an indication that between pH 6.6 and 8.0 the HeLa metaphase cells possessed a much greater capacity than the Don metaphase cells to induce prophasing. Fusion of Don metaphase cells with HeLa interphase cells or of Don interphase cells with HeLa metaphase cells at pH 8.0 resulted in nuclear envelope formation or prophasing in each kind of heterokaryon. As in the homokaryons, the frequencies of the two events in the heterokaryons depended on the metaphase/interphase ratio. The statistics of prophasing and nuclear envelope formation in the homo- and heterokaryon populations were consistent with the notion that disruption or formation of the nuclear envelope depends on the balance attained between disruptive and formative processes.


Autoradiographic studies of synthesis and intracellular migration of glycoproteins in the rat anterior pituitary gland. The incorporation of [(3)H]fucose in the somatotrophic and gonadotrophic cells of the rat adenohypophysis has been studied by electron microscope autoradiography to determine the site of synthesis of glycoproteins and to follow the migration of newly synthesized glycoproteins. The pituitaries were fixed 5 min, 20 min, 1 h, and 4 h after the in vivo injection of [(3)H]fucose and autoradiographs analyzed quantitatively. At 5 min after [(3)H]fucose administration, 80-90% of the silver grains were localized over the Golgi apparatus in both somatotrophs and gonadotrophs. By 20 min, the Golgi apparatus was still labeled and some radioactivity appeared over granules. At 1 h and 4 h, silver grains were found predominantly over secretory granules. The kinetic analysis showed that in both protein-secreting cells (somatotrophs) and glycoprotein-secreting cells (gonadotrophs), the glycoproteins have their synthesis completed in the Golgi apparatus and migrate subsequently to the secretory granules. It is concluded from these in vivo studies that glycoproteins which are not hormones are utilized for the formation of the matrix and/or of the membrane of the secretory granules. The incorporation of [(3)H]fucose in gonadectomy cells (hyperstimulated gonadotrophs) was also studied in vitro after pulse labeling of pituitary fragments in medium containing [(3)H]fucose. The incorporation of [(3)H]fucose was localized in both the rough endoplasmic reticulum (ER) and the Golgi apparatus. Later, the radioactivity over granules increased while that over the Golgi apparatus decreased. The concentration of silver grains over the dilated cisternae of the rough ER was not found to be modified at the longest time intervals studied.


Intracellular binding of ruthenium red in frog skeletal muscle.


Assembly of gap junctions during amphibian neurulation. Sequential thin-section, tracer (K-pyroantimonate, lanthanum, ruthenium red, and horseradish peroxidase), and freeze-fracture studies were conducted on embryos and larvae of Rana pipiens to determine the steps involved in gap junction assembly during neurulation. The zonulae occludentes, which join contiguous neuroepithelial cells, fragment into solitary domains as the neural groove deepens. These plaque-like contacts also become permeable to a variety of tracers at this juncture. Where the ridges of these domains intersect, numerous 85-A participles apparently pile up against tight junctional remnants, creating arrays recognizable as gap junctions. With neural fold closure, the remaining tight junctional elements disappear and are replaced by macular gap junctions. Well below the junctional complex, gap junctions form independent of any visible, preexisting structure. Small, variegated clusters, containing 4-30 particles located in flat, particle-free regions, characterize this area. The number of particles within these arrays increases and they subsequently blend together into a polygonally packed aggregate resembling a gap junction. The assembly process in both apical and basal regions conforms with the concept of translational movement of particles within a fluid plasma membrane.


Peptide and amine producing endocrine-like cells in the chicken thymus. A chemical, histochemical and electron microscopic study.


Studies on 5'-nucleotidase histochemistry. I. 5'-Nucleotidase activity in the developing esophagus of the mouse.


Some effects of salts on staining: use of the Donnan equilibrium to describe staining of tissue sections with acid and basic dyes.


Electron microscopy and diffraction of wet unstained and unfixed biological objects.


Thiosulphation for the histochemical demonstration of protein-bound sulphydryl and disulphide groups.


Renal glycoprotein: glycosyl transferase activities after administration of folic acid.


The deposition of dental plaque in young adults on a diet containing chocolate and skim-milk powder.


Alpha-fetoprotein levels in amniotic fluid in pregnancies complicated by rhesus isoimmunization.


Formamidase in rat brain.

Formamidase in rat brain.


Diamorphine-induced attack of paroxysmal hypertension in phaeochromocytoma.


Psychotic "art" in New Guinea. A cross-cultural study.


The recovery cycle of the averaged auditory evoked response during sleep in normal children.


Cerebellar responses to penicillin-induced cerebral cortical epileptiform discharge.


Intra-hemispheric phase relationships during self-regulated alpha activity.


Age and sex differences in averaged evoked responses in a normal population, with observations on patients with gonadal dysgenesis.


Contamination of scalp EEG spectrum during contraction of cranio-facial muscles.


An implantable multi-channel biotelemetry system.


Alpha wave phase and alpha attenuation.

Alpha wave phase and alpha attenuation.


The recovery cycle of the averaged auditory evoked response during sleep in autistic children.


An electrode for recording single motor unit activity during strong muscle contractions.


A simple eye movement monitoring system for clinical electroencephalography.


A dual digital integrator for EEG studies.


A technique for feature extraction and interpatient comparison of H-reflex conditioning curves.


An electronic device for continuous counting of chemically induced epileptic discharges.


Escherichia coli antibody: a screening test for immunodeficiency. Six patients suffering from recurrent chest infections were found to lack antibodies to a pooled antigen obtained from six different serotypes of commensal Escherichia coli bacteria. All had normal serum IgG concentrations, but five subsequently benefited from regular gammaglobulin injections. We suggest that the absence of such E. coli antibodies usually indicates a clinically significant defect in antibody production. This simple screening test is of use in the diagnosis of primary and secondary immunodeficiency disorders.


Fractionation and purification of prostatic acid phosphatase.


Preventive dentistry: fads and facts.

Preventive dentistry: fads and facts.


Androgen assimilation by normal and hyperplastic dog prostate glands.


Homologous radioimmunoassay of thyrotrophin in rat plasma.


Gilbert Frewin Hamilton.

Gilbert Frewin Hamilton.


Immunology of the tears.

Immunology of the tears.


Regional variation in the permeability of rat thoracic aorta. The permeability and ultrastructure of the intercostal orifices and interostial regions of rat thoracic aorta have been compared in the present study. The ostial regions consist of two different portions, an annulus and an inflow tract, the latter being continuous with the intercostal artery. The wall of the inflow tract has a sharp bend, demarcating inner and outer portions. Faint blueing of the lateral portions of aortic annuli is visible from 3(1/2) to 4(1/2) hours after injection of Evans blue dye and is no longer apparent at 5(1/2) hours, when there is diffuse discoloration of the thoracic aorta. Only the annuli are labeled by horseradish peroxidase (HRP) for 1(1/2) to 3(1/2) minutes after intravenous injection. There is more extensive and progressively heavier labeling of annuli and inner halves of the inflow tracts between 5 to 12 minutes. HRP is transported across aortic endothelium within micropinocytotic vesicles. Intercellular endothelial clefts do not appear to be directly permeated from the lumen through their junctions for a period of at least 3(1/2) minutes. The present observations preclude an assessment of the penetration of the junctions by HRP at later intervals. No appreciable differences are observed in the mode of transport of peroxidase through the more permeable endothelium of the annulus and less permeable endothelium of inflow tract and interostial regions. Observations made on tissues exposed to colloidal lanthanum, and on specimens stained en bloc with uranyl acetate and subjected to a combination of rotation-tilting, fail to demonstrate appreciable differences in the ultrastructure of the endothelial cell junctions of intercostal orifices and interostial regions. Since morphologic differences do not seem to account for the greater permeability of annular regions, the role of hemodynamic forces is considered. The relationship between regional variation in permeability and the development of atherosclerotic lesions is also discussed


The fatty acid composition of cerebrosides and sulfatides in a case of adult metachromatic leukodystrophy.


Enrichment in B cells after separation of lymphocytes from peripheral blood.


Electrophoretic studies on the variations in serum protein profiles of golden hamsters infected experimentally with Opisthorcis felineus (Rivolta, 1884): globulin fractions.


Banding analysis of the chromosomes of Marmosa mitis (murine opossum).


Purification of a specific antibody to rat liver malic enzyme by affinity chromatography and application of the antibody as a quantitative reagent.


The relation of millisecond delayed light emission to light-induced ion accumulations in chloroplasts.


Electron microscopic demonstration of rat liver glycogen phosphorylase activity with iron (Fe++).


Histological and ultrastructural study of the glial pigment in pallidum and striatum of man.


New studies on the neural crest origin of the avian ultimobranchial glandular cells--interspecific combinations and cytochemical characterization of C cells based on the uptake of biogenic amine precursors.


Scanning electron microscopy: identification of mast cells by indirect cathodoluminescence.


A quantitative approach for immunofluorescence in microscopy: the use of antibody multilayers on nuclei.


Morphological and histochemical observations of the regenerated mucosa of the duodenum of the fowl after sub-total villous atrophy.


Glycoprotein: glycosyl transferase activities of rat kidney plasma membranes.


Iodine metabolism in leukocytes: effect of graded iodide concentrations.


Rapid detection of hypoxanthine-guanine phosphoribosyltransferase on cellogel.


DNA coating of blood vessels in retinoblastomas.


Mammalian cytogenetics. 8. Q-banding of chromosomes of Pan troglodytes, Gorilla gorilla berngei and Pongo pygmaeus.


Follicular lymphoma with deposits of amorphous hyaline material.


Alpha-chain disease with clinical, immunological, and histological recovery. Clinical, immunological, and histological recovery in a patient with alpha-chain disease is described. The patient, a 27-year-old Greek man, presented with severe steatorrhoea, abdominal pain, oedema, and hypogammaglobulinaemia. Treatment with tetracycline produced only temporary remission. Intermittent therapy with prednisone and cyclophosphamide together with antibiotics was followed by clinical recovery, return of histological appearances of the small intestine to normal, and disappearance of free alpha-chain protein from the serum. The patient remained well one year later without treatment.


Heterochromatin distribution and chiasma localization in the grasshopper Btyodema tuberculata (Fabr.) (Acrididae).


Selective staining of cell nuclei with haematoxylin containing transitional metal cations.


Autosomal reciprocal translocations in newborn children and their relatives.


On the mechanism of T-cell mediated cytolysis.


Turner phenotype: mosaic 45,X-47,XY, plus 18.


Mucopolysaccharides histochemistry of Musca domestica. IX. The oenocytes.


The hatching process in fish embryos. 3. The structure, polysaccharide and protein cytochemistry of the chorion of the trout egg, Salmo gairdneri (Rich.).


Histochemical studies on the mucins of the vertebrate tongues. V. Comparative mucopolysaccharide histochemistry of the mast cells in the tongues of twenty vertebrates.


Familial hyperparathyroidism.

Familial hyperparathyroidism.


The quantitative partition of the albumin fraction of milk serum proteins by gel chromatography.


Changes of internal elastic membrane in the coronary arteries as related to atherosclerotic involvement.


Specificities of antisera against testosterone linked to albumin at different positions (C3, C11, C17).


Muscle biopsy--technical and diagnostic aspects.


Current thoughts on management of hyperthyroidism.


Progesterone in the management of hormone-dependent inoperable tumours.


Serological properties and amino acid composition of the fimbriae of Aeromonas hydrophila.


Loss of small peripheral sensory neurons in Fabry disease. Histologic and morphometric evaluation of cutaneous nerves, spinal ganglia, and posterior columns.


A comparative study on rat liver and hepatoma nuclear membranes.


The ion exchanger character of insoluble collagen fraction isolated from bovine nasal septal cartilage.


Sex hormones--effects on prolactin cells.


The passive cutaneous anaphylaxis test (PCA) in the diagnosis of allergic respiratory diseases caused by hypersensitivity to organic dust.


Biochemical aspects of drug combinations.


Carbon monoxide hazard in Cairo traffic.


Editorial: Steatosis-Mallory's hyaline-cirrhosis: Can their relationships be resolved by an experiment of nature?


Liver ultrastructure in abetalipoproteinemia: Evolution of micronodular cirrhosis.


Letter: Identification of two populations of human lymphocytes.


Specificity differentiation of herpes simplex virus types 1 and 2 by indirect immunofluorescence.


Identification of Streptococcus mutans colonies by mannitol-dependent tetrazolium reduction.


Fluorescamine: a novel reagent for the histochemical detection of amino groups.


Hodgkin's disease 1974.

Hodgkin's disease 1974.


Influence of the mutation "diabetes" on insulin release and islet morphology in mice of different genetic backgrounds. Mice, 7-8-mo old, of the C57BL/KsJ-db strain and homozygotic for the mutant gene db, exhibited marked hyperglycemia and moderately elevated serum insulin levels. Light and electron microscopy provided evidence of a slightly decreased proportion of beta cells in the pancreatic islets, irregular islet architecture with intraislet ducts, and degenerative as well as hypertrophic changes in the individual beta cells. As a rule, islets microdissected from these mice did not release insulin in response to glucose, theophylline, iodoacetamide, or chloromercuribenzene-p-sulphonic acid. The absence of secretory responses was not simply due to lack of insulin. Although the islet content of insulin was decreased in C57BL/KsJ-db/db mice, the remaining amount was severalfold larger than that released from stimulated islets of normal controls. Another mutation, db(2J), an allele of db with identical phenotypic expressions in the C57BL/KsJ strain, was studied on the genetic background C57BL/6J. In contrast to the severely diabetic C57BL/KsJ-db/db animals, the C57BL/6J-db(2J)/db(2J) mice were characterized by highly elevated serum insulin levels and only moderate hyperglycemia. Their endocrine pancreas was enlarged and showed an increased proportion of beta cells. Like the islets of normal mice, those of C57BL/6J-db(2J)/db(2J) mice responded to glucose and chloromercuribenzene-p-sulphonic acid, the glucose-induced responses being potentiated by theophylline or iodoacetamide. C57BL/KsJ-db/db mice should provide a valuable model for studying defects in insulin secretion in relation to diabetes mellitus. Mice of the C57BL/6J strain offer a control material that may help to elucidate the dependence of the insulin secretory defect on the background genome.


Investigation of a cell strain with trisomy 14 from a spontaneously aborted human fetus.


Avian oral pigmentation.

Avian oral pigmentation.


Gingival fluid in relation to tooth mobility and occlusal interferences.


The identification of adrenergic and cholinergic nerve endings in the trabecular meshwork.


The effect of bleomycin and radiation in combination on bacteria and mammalian cells in culture.


The distribution of repetitive DNA in the chromosomes of cultured cells of Drosophila melanogaster.


End results of breast cancer patients in Finland 1953-1968.


Breast milk: a source of more than nutrition for the neonate.


Histamine release from mast cells: role of microtubules.


Significance of postradiation bronchoscopical findings in the prognosis of bronchial carcinoma.


Michael Scot and Mary of Bologna. A medieval gynecological puzzle.


Defective translational control in the beta-thalassemia of Ferrara.


A review of the genus Chironomus (Diptera, Chironomidae). IX. The cytology of Chironomus tepperi Skuse.


DNA replication and RNA transcription of euchromatic and heterochromatic chromosome regions during grasshopper meiosis.


The DNA content of sperm and hemocyte nuclei of the silkworm, Bombyx mori L.


DNA replication, G- and C-bands and meiotic behaviour of supernumerary chromosomes of Rattus rattus (Linn.).


Chromosomal banding patterns and karyotype evolution in three pig kidney cell strains (PK15, F and RP).


Euploid segregation through multipolar mitosis in mammalian cell cultures. Identification of triploid, haploid, and segregating diploid cells in a diploid-euploid primary culture of rhesus kidney cells.


Immunological studies on chimpanzee plasma proteins.


A monolayer and freeze-etching study of charged phospholipids. I. Effects of ions and pH on the ionic properties of phosphatidylglycerol and lysylphosphatidylglycerol.


Metastatic basal cell carcinoma: review, report of a case, and chemotherapy.


Immunoregulatory alpha-globulins (IRA) and tumor immunity.


DNA polymerases in human lymphoblastoid cells infected with simian sarcoma virus.


Enzyme histochemical studies of autotransplanted nasal septum in the rat.


Eye defects of mentally handicapped children. Among 171 children who received routine ophthalmic and orthoptic examinations as part of their evaluation for suspected or known developmental handicap it was found that there was an unusually high incidence of ocular defects. Especially prevalent were high refractive errors (52%) and squints (40%), but there were also a number of other serious eye defects ranging from nystagmus and cataracts to microphthalmos.Because of this high incidence of eye defects it was considered essential that ophthalmic and orthoptic examinations should be part of the routine evaluation of developmentally handicapped children by the team of workers in an assessment centre.


Corticosteroid therapy of preterminal gastrointestinal cancer.


DNA complementary to globin and immunoglobulin mRNA: a probe to study gene expression.


Tribenoside as an inhibitor of chemically induced histamine release.


The intensification of cobalt-filled neurone profiles using a modification of Timm's sulphide-silver method.


Aryl-sulfatase activity of membrane-coating granules with particular reference to their origin.


Cyclic adenosine monophosphate, adenyl cyclase and cyclic nucleotide phosphodiesterase in psoriatic epidermis, with a comment on urinary cAMP.


Dopa and 5-S-cysteinyldopa in malignant melanoma in Ugandan Africans.


5-S-cysteinyldopa in melanomas of Caucasians.


Ultrastructure of systemic lupus erythematosus. Dermal connective tissue.


Connective-tissue effects of drugs causing lupoid syndrome.


Erythrohepatic protoporphyria and its treatment with beta-carotene.


Iron metabolism in tetracycline-treated acne patients.


The effects of polythene occlusion and topical corticosteroids on resistance to friction injury in the skin.


Erythema dyschromicum perstans (ashy dermatosis). Report of two cases from Scandinavia.


Generalized plane xanthomas and monoclonal IgG.


Metachromatic reaction of pseudoisocyanine with sulfatides in metachromatic leukodystrophy (MLD). I. Technique of histochemical staining.


Structure and development of viruses as observed in the electron microscope. XI. Entry and uncoating of herpes simplex virus. Two morphologically distinct types of capsids are described. The dense capsid appeared to be disrupted near the cellular membrane with release of core material. The light capsid was more stable and was frequently encountered close to the nucleus, where empty capsids were also found. Pretreatment of cells before infection with either puromycin or actinomycin D markedly decreased the percentage of empty capsids. It is suggested that the two types of capsids play different roles in the process of initiating infection. One (the dense capsid) releases deoxyribonucleic acid (DNA) shortly after entry. This DNA is transcribed into a virus-specific ribonucleic acid, which codes for an enzyme capable of altering the permeability of the second type of capsid (the light capsid). In proximity to the nucleus, the infectious DNA then escapes without gross disruption of the capsid.


Oral manifestations of hypogammaglobinaemia in a young child.


Component of strain MC29 avian leukosis virus with the property of defectiveness. Three clones of morphologically altered cells (L(-)MC29) of singular properties were isolated from MC29 (subgroup A) leukosis virus-infected chick embryo cells. Supernatant fluids from cultures of the cloned cells produced no transforming or interfering activity on chick embryo cells susceptible to known avian leukosis-sarcoma viruses. No virus associated with the cells was demonstrable by fluorescent-antibody staining or by electron microscopy. All L(-)MC29 clone cells were activated, however, by four strains of Rous-associated viruses (RAV) representative of A, B, C, and D subgroup avian leukosis viruses and by two strains of MC29 virus. Virus L(-)MC29 cells activated by superinfection with RAV-1 and RAV-2 was characterized by helper-dependent and helper-independent properties. These findings suggest that the strain MC29 leukosis virus, or a component thereof, possesses properties of defectiveness similar to those of the Bryan high-titer Rous sarcoma virus.


Correlation of structural differences in several bird lysozymes and their loop regions with immunological cross-reactivity.


The use of acid-isopropyl alcohol in the acid-fast staining procedure.


The effect of racin on the ultrastructure of the kidney.


Interference with healing of rat skin incisions treated with chlorhexidine.


Studies on guinea pig skin cell cultures. II. Effect of a pig skin extract on DNA synthesis.


Trace elements with suspected and hitherto unknown biological function in normal and psoriatic epidermis.


Contamination of leg ulcers. A bacteriological and clinical study on the effect of treatment with an ointment containing ristocein A and circulin.


Implantation dermatoses.

Implantation dermatoses.


Erythema dyschromicum perstans. A report of two cases in fair-skinned patients.


Acquired (digital) fibrokeratomas. Complication of ingrown toenail.


Scleredema.

Scleredema.


Compatibility of hormonal contraceptives in diabetics. Results of a questionnaire survey.


A comparison of methods for labelling DNA for use in the radioimmunoassay of DNA-antibodies.


A buffered chromic chloride method of attaching antigens to red cells: use in haemagglutination.


Kinetic studies of gene frequency. I. Use of a DNA copy of reticulocyte 9 S RNA to estimate globin gene dosage in mouse tissues.


Media systems for education and training in the allied health sciences.


Do-it-yourself slides for projection: an easy way.


Kinetic studies of gene frequency. II. Complexity of globin complementary DNA and its hybridization characteristics.


Serial maternal serum alpha 1-fetoprotein concentration during induced abortions.


A comparative study for ultrastructural localization of intracellular immunoglobulins using peroxidase conjugates.


Fluorometric scanning of fluorescamine-labeled proteins in polyacrylamide gels.


Localization within cloned rat pituitary tumor cells of material that binds antigrowth hormone antibody.


Skeletal changes accompanying congenital indifference to pain in two Chinese children.


Ultrastructural immunocytochemical localization of lysozyme in the Paneth cells of man.


Histochemical properties of the antral gastrin cell.


Developmental changes in the synthesis of alpha-fetoprotein and albumin in the mouse liver. Cell-free synthesis by membrane-bound polyribosomes.


Evidence for a microviscosity increase in the Escherichia coli cell envelope caused by colicin E1.


Ribonucleic acid dependent deoxyribonucleic acid synthesis by Escherichia coli deoxyribonucleic acid polymerase. I. Characterization of the polymerization reaction.


Tumor virus ribonucleic acid directed deoxyribonucleic acid synthesis: nucleotide sequence at the 5' terminus of nascent deoxyribonucleic acid.


Frequency of deletion of short arm satellites in acrocentric chromosomes.


Translocation, t(4qminus;13qplus), in three generations resulting in partial trisomy of the long arm of chromosome 4 in the fourth generation.


Letter: Cellular metachromasia with toluidine blue O in cultured white cells of cystic fibrosis heterozygotes.


New trends in the chemotherapy of tuberculosis--current aspects.


Diploid, tetraploid and octaploid beta cells in the islets of Langerhans of the normal human pancreas.


Viral RNA of murine sarcoma virus produced by a hamster-mouse somatic cell hybrid.


Inhibition of cell-dependent cytotoxicity as an assay for mouse alloantibody.


Minimal bone marrow damage in mice given bleomycin.


Changes in the affinity of phosphotungstic acid and positively charged colloidal particles for the surfaces of malignant human transitional epithelium of the urinary bladder.


Proceedings: Phase-specific oncocolon antigens: a theoretical framework for "carcinoembryonic antigen" specificities.


Binding of progesterone in the human Fallopian tube. The uptake, subcelluar distribution, and other characteristics of progesterone binding in the human fallopian tube are presented.  65% of the radioactivity of tritiated progesterone was found in the fraction composed of nuclei, cell membranes, unbroken cells and heavy particulate matter after centrifugation.  Mitochondria and microsomes had about 25% of the total radioactivity.  A dissociation constant (Kd) of 5.9 x 10 to the minus 10 M was determined for the steroid binding proteins, with a probable concentration of total cytoplasmic binding sites for progesterone in each fallopian tube of 1.26 pmoles.


A quantitative study of the pregnancy zone protein in the sera of pregnant and puerperal women.


Prenatal sex determination by fluorescent staining of cells for the presence of Y chromatin. The fluorescent staining method for determing the sex of the fetus is evaluated.  Midcervical smears were obtained from 20 women who had elected a midtrimester abortion.  The smears were stained with quinacrine hydrochloride and examined under a fluorescent microscope.  8 smears showed intranuclear fluorescent bodies that differed from typical Y chromatin.  There was more than 1 spot in these smears, and they occurred with equal frequency in smears from mothers of both male and female aborted fetuses.  Of the 8 smears with fluorescent spotting, 5 were male, and of the 12 smears without spots, 3 were male.  Smears and sections prepared from the chorionic surface of 5 male and 5 female placentae showed inconsistent fluorescent spots, and the spots occurred with equal frequency in males and females.  It was concluded that the multiple fluorescent spots in the nuclei make it almost impossible to distinguish Y chromatin and that the cells obtained from the midcervical smears do not afford a reliable predictor for prenatal sex determination.


Repair in arterial tissue. A scanning electron microscopic (SEM) and light microscopic study on the endothelium of the rabbit thoracic aorta following noradrenaline in toxic doses.


Intraventricular aberrancy versus ventricular ectopy.


Effects of colchicine on myofilament arrangement and the lysosomal system in skeletal muscle.


Cytochemical demonstration of catalasepositive particles (peroxisomes?) in fibroblasts.


[Morphogenesis of the respiratory epithelium in the tracheal gills of larval Limmephilini KOL. (Insecta, Trichoptera) (author's transl)].