The protection against the cerebral and pulmonary effects of hyperbaric oxygen by pharmacological agents and lithium salts in mice.


Characterization of viruses (Witwatersrand and Germiston) isolated from mosquitoes and rodents collected near Lunyo Forest, Uganda, in 1968.


Ankle-disarticulation: a definitive type of amputation in adults.


Electron microscopic and enzyme histochemical studies of cerebellum, ocular and skeletal muscles in chronic progressive ophthalmoplegia with cerebellar ataxia.


The effect of fluroxene on intracranial pressure in patients with intracranial space-occupying lesions.


A look to the future.

A look to the future.


Inhibition of the effect of oxytocin by methoxyflurane in vitro.


Effect of premedication on duration of anesthesia with halogenated vapours: chloroform, trichlorethylene, halothane, methoxyflurane, enflurane (Ethrane) and isoflurane (forane).


The effect on dental caries of weekly rinsing with a neutral sodium fluoride or an acidulated phosphate-fluoride mouthwash.


Concentrations of gentamicin and carbenicillin in bronchial secretions.


Studies on the stereospecificity of closely related compounds which block postganglionic acetylcholine receptors in the guinea-pig ileum.


Synthesis of some N-substituted eleostearic acid hydrazides as potential monoamine oxidase inhibitors.


Selectivity in new  -adrenergic blocking agents. (3-Amino-2-hydroxypropoxy)benzamides.


Derivative of 3,4-dihydro-1(2H)-naphthalenone as  -adrenergic blocking agents. 3. Carbonyl-containing analogs of bunolol.


An oral health survey of Aborigines and Torres Strait Islanders in far North Queensland.


Adrenergic mechanisms in the cerebral vascular bed.


An improved method for the study of reagin-mediated mast cell degranulation in rats.


Regulation of tyrosine aminotransferase in rat liver. X. Characterization and interconversion of the multiple enzyme forms.


Regulation of tyrosine aminotransferase in rat liver. XI. Studies on the relationship of enzyme stability to enzyme turnover in cultured hepatoma cells.


Specific basic proteins from mammalian testes. Isolation and properties of small basic proteins from rat testes and epididymal spermatozoa.


3-Hydroxy-3-methylglutaryl coenzyme A reductase. Solubilization and purification of a cold-sensitive microsomal enzyme.


Electrophysiological and antiarrhythmic effects of 4-(2-hydroxy-3-isopropylaminopropoxy)-indole (LB-46) on the canine cardiac cells.


Properties of chorismate synthase in Neurospora crassa.


Reaction of antiarrhythmic agents with aglycones and erythrophleum alkaloids.


Preparations for morning sickness.

Preparations for morning sickness.


Antiarrhythmic potency of N-propyl ajmaline with untoward response of ventricular fibrillation in excess dose.


Transplantation immunity evoked by skin homografts and expressed in intact skin.


An analysis of epidermolysis in hamsters suffering from homologous disease.


Liver metabolic reactions: tertiary amine N-dealkylation, tertiary amine N-oxidation, N-oxide reduction, and N-oxide N-dealkylation. II. N,N-dimethylaniline.


Preperitoneal repair of inguinal hernias in infancy and childhood.


The bacterial degradation of flavonoids. Hydroxylation of the A-ring of taxifolin by a soil pseudomonad. Cell-free extracts prepared from a Pseudomonas sp. grown on (+)-catechin oxidized taxifolin in the presence of NAD(P)H and molecular oxygen. The enzyme catalysing the reaction was partially purified and shown to be a flavoprotein. The product was identified as 3',4',5,7,8-pentahydroxyflavanonol (dihydrogossypetin).


Conversion of p-coumarate into caffeate by Streptomyces nigrifaciens. Purification and properties of the hydroxylating enzyme. 1. An enzyme responsible for the conversion of p-coumarate into caffeate was purified 97-fold from Streptomyces nigrifaciens. The enzyme had a molecular weight of 18000 as determined by Sephadex G-100 gel filtration and was homogeneous on polyacrylamide-gel electrophoresis. 2. The preparation exhibited both p-coumarate hydroxylase and caffeate oxidase activities. 3. Stoicheiometry of the reaction indicated a mono-oxygenase-mediated catalysis consuming 1mol of O(2)/mol of substrate hydroxylated. 4. NADH, NADPH, tetrahydropteroylglutamate or ascorbate act as electron donors for the reaction, ascorbate being inhibitory at higher concentrations. 5. The optimum enzyme activity was at about pH7.7 and 40 degrees C, with an activation energy of 39kJ/mol. 6. Monophenols such as p-hydroxyphenylpropionate, p-hydroxyphenylacetate, l-tyrosine and dl-p-hydroxyphenyl-lactate were also hydroxylated by the preparation, in addition to p-coumarate. 7. The enzyme was a copper protein having 0.38% copper in a bound form. 8. Thiol-group inhibitors did not affect the reaction. 9. The relationship of the enzyme to other hydroxylases is discussed.


Amino acid sequence of the N-terminal sixty-nine residues of heavy chain derived from a homogeneous rabbit antibody. The sequence of the N-terminal 69 residues of heavy chain from a homogeneous rabbit antibody to type III pneumococcal polysaccharide was determined. The sequence is similar to that found in heavy chains of normal pooled rabbit immunoglobulins of the same allotype Aa1. Two regions of the homogeneous heavy chain (residues 35-46 and 62-69) are very similar to corresponding regions of heavy chains from rabbit Aa2 immunoglobulin, as well as from mouse, guinea-pig and human immunoglobulins. In contrast, residues 47-62 appear to be variable. Comparison in this section with another homogeneous anti-pneumococcal antibody (Strosberg et al., 1972) of related specificity and of the same allotype indicates sequence variation in at least three positions. An antibody to group C streptococcal carbohydrate of allotype Aa2 (Fleischman, 1971) differs by five amino acids in the same region of the heavy chain. Sequence variability between these three antibodies does not occur in homologous positions within this variable section. Allotype-related sequences could not be identified in section 34-65.


Drug therapy for the reduction of gastric acid secretion.


Inhibition of erythropoiesis in the spleens of irradiated mice injected with allogeneic lymphocytes. Reactivity of lymphocytes aganist non-H-2 transplantation antigens.


Haemodynamic effects of a new beta-blocking agent 'Sectral' (M & B 17803A).


Biogenic amines and endocrine function. Introduction: neuroendocrine transducers and monoamines.


Control of epinephrine synthesis and secretion.


Ambulance service.

Ambulance service.


Biogenic amines, sympathetic nerves, and renin secretion.


Idiosyncracies of parathyroid hyperfunction.


Regulation of insect populations by protozoa and nematodes.


Phosphonoprotein. Characterization of aminophosphonic acid rich glycoproteins from sea anemones.


An enzymic reduction system for metmyoglobin and methemoglobin, and its application to functional studies of oxygen carriers.


Influence of nicotinamide and pyridine nucleotides on streptozotocin and alloxan-induced pancreatic B cell cytotoxicity.


Insulin metabolizing enzyme activities in human placental tissue.


Iron-dependent loss of liver cytochrome P-450 haem in vivo and in vitro.


NADP specific dihydroxyacetone reductase from Dunaliella parva.


Antibodies to plant ferredoxin.

Antibodies to plant ferredoxin.


Medicine: the learned--and learning--profession.


Respiratory protection of nitrogenase in Azotobacter vinelandii.


Clearance of foreign red cells from the blood of aging mice.


Which are witch?

Which are witch?


VD an equal opporunity destroyer.

VD an equal opporunity destroyer.


Migrant health care--an attempt to meet the problem.


National cooperative study of hepatitis B antibody treatment of antigen-positive fulminant hepatitis: first randomized case in Illinois.


EKG of the month: Junctional premature beat.


Ectopic hydroureter.

Ectopic hydroureter.


Emergency department physician staffing patterns and residency training.


Ruptured spleen.

Ruptured spleen.


Colerectal adenomas and adenocarcinomas.


Medicine in 1900. The physician as the guardian of the public health and his contributions to charity.


Study of serum acid phosphatase in children with kidney diseases.


Multiplication of viruses isolated from arthopods and vertebrates in Australia in experimentally infected mosquitoes.


A commentary on "Current Studies on Schizophrenia".


Field studies using wide-mesh mosquito bed nets in Taiwan and Indonesia.


The presence of a peritrophic membrane in pupal mosquitoes (Diptera: culicidae).


A comparison of the neural regulation of tyrosine hydroxylase activity in sympathetic ganglia of adult mice and rats.


Feedback regulation of 5-HT synthesis in rat striatal slices.


The estimation of acetylsalicylic acid and salicylate in biological fluids by gas-liquid chromatography.


Rapid gas-liquid chromatographic estimation of antipyrine in plasma.


The oxidation of 6-hydroxydopamine.

The oxidation of 6-hydroxydopamine.


Further studies on the adrenergic neuron blocking activity of some  -adrenoceptor antagonists and guanethidine.


A circular dichroic investigation of the binding of fenoprofen, 2(3-phenoxyphenyl)-propionic acid, to human serum albumin.


Analysis of the effects of apomorphine and bulbocapnine in relation to the proposed dopamine receptor.


Polyphloretin phosphate temporarily potentiates prostaglandin E 2  on the rat fundus, probably by inhibiting PG15-hydroxydehydrogenase.


The pharmacokinetics of phentermine and chlorphentermine in chronically treated rats.


Hydrophilic-lipophilic balance and partial molal volume of non-ionic surfactants.


Oscillatory testing of o-w emulsions containing mixed emulsifers of the surfactant-long chain alcohol type: self-bodying action.


Prediction of systemic availability from plasma-level data after oral drug administration.


A comparison of the pore structure of tablets prepared from force screened and comminuted granules.


The influence of synthetic "substance P" on tremor caused by LSD in mice.


Modification by caerulein of action potential activity in circular smooth muscle of isolated small intestine.


On the question of subclassification of  -adrenoceptors.


Evidence for carrier-mediated efflux of noradrenaline from the axoplasm of adrenergic nerves in rabbit atria.


Differential effects on mouse brain catecholamine turnover of chlorpromazine, trifluoperazine and closely-related non-tranquillizing analogues.


The absorption and distribution of isopropamide iodide in the rat.


Stereospecific requirements for hallucinogenesis.


Comparison of dental surveys in Elmira and Corning, New York.


Polyarthritis induced in the rat with cell walls from several bacteria and two Streptomyces species.


Well-women.

Well-women.


Incisor caries and primary school tuckshops.


Differential certification of physician's assistants.


The role of a regional surgical society.


EPR studies on phosphorylating particles from Azotobacter vinelandii.


Stoichiometry of reduction and phosphorylation during illumination of intact chloroplasts.


Antimycin-insensitive mutants of Candida utilis. I. Isolation and characterization of mutant 28.


California encephalitis virus endemicity in the Yukon Territory, 1972. Sera from 218 of 1574 (14%) small mammals collected in the Yukon Territory between 14 May and 13 August 1972 neutralized a Yukon strain of California encephalitis virus (snowshoe-hare subtype). These included 133 of 319 (42%) snowshoe hares (Lepus americanus), 84 of 1243 (7%) ground squirrels (Citellus undulatus) and 1 of 12 (8%) tree squirrels (Tamiasciurus hudsonicus). California encephalitis virus (snow-shoe hare subtype) was isolated from four pools of unengorged Aedes communis mosquitoes collected near Whitehorse (61 degrees N., 135 degrees W.) and on one occasion each from pools of the same species collected at Hunker Creek (64 degrees N., 138 degrees W.) and at mile 125, Dempster Highway (66 degrees N., 138 degrees W.) during July 1972. Replication of a Yukon strain of California encephalitis virus was observed in wild-caught Culiseta inornata and Aedes canadensis mosquitoes after intrathoracic injection and holding at temperatures of 80 degrees , 50 degrees and 40 degrees F.


Role of bacterial endotoxin in the graft-vs.-host syndrome.


Possible role of endotoxemia during immunologic imbalance.


The future of the United States space program. The 1973 Harvey Cushing oration.


Relief of postherpetic neuralgia with psychotropic drugs.


Growth and metabolism of rats fed nonprotein nitrogen diets supplemented with nicotinamide, succinate and pyridoxine.


Evaluation of electrical pulses and elevated levels of potassium ions as stimulants of adenosine 3', 5'-monophosphate (cyclic AMP) accumulation in guinea-pig brain.


Effects of hormones, prostaglandins and cyclic-AMP on the synthesis of progesterone by the human placenta in vitro.


Treating problem children with stimulant drugs.


Risks vs. benefits of antipsychotic drugs.


The role of beta adrenoceptors in the coronary and systemic hemodynamic responses to emotional stress in conscious dogs.


Practolol in treating tachyarrhythmias.

Practolol in treating tachyarrhythmias.


A microsomal enzyme inhibitor in the gut contents of the house cricket (Acheta domesticus).


Antimicrobial agents--mechanisms of action and clinical usage.


Purification & properties of a soluble reduced nicotinamide adenine dinucleotide (NADH) dehydrogenase from Agrobacterium tumefaciens.


Protein deficiency & regional chemistry of the brain. II. Effects of protein deficiency on regional distribution of enzymes of glutamate metabolism in rat brain.


Purification and properties of beef liver D-glycerate dehydrogenase.


Identity of the rapidly appearing purple intermediate of D-amino acid oxidase.


Effects of Triton X-100 on drug hydroxylation system of rat liver microsomes induced by phenobarbital or 3-methylcholanthrene.


The early stage of labeling of microsomal membrane proteins in rat liver by radioactive amino acids.


Physiological and pharmakological influences on orthostasis.


The role of ABO blood groups in blood rheology of cardiovascular disorders.


Effects of interferon on leukaemia virus activation in graft versus host disease.


Drugs ingested by pregnant women.

Drugs ingested by pregnant women.


Drug metabolism by placenta.

Drug metabolism by placenta.


Glutamine synthetase induction in embryonic neural retina: immunochemical identification of polysomes involved in enzyme synthesis. Glutamine synthetase (EC 6.3.1.2) in embryonic neural retina in culture is rapidly induced by hydrocortisone. Retina polysomes involved in translation of this enzyme were precipitated with a high degree of specificity by the gammaglobulin isolated from antiserum against the enzyme (anti-enzyme gammaglobulin). Using immunoprecipitation procedures, we determined that the amount of polysome-bound nascent enzyme was maximal in polysomes comprising 9-14 ribosomes and was about 3-fold higher in the induced than in the noninduced retina. Within this size group of polysomes, those comprising 11-13 ribosomes showed consistently greater binding of anti-enzyme [(125)I]gammaglobulin than of normal [(125)I]-gammaglobulin. This size of polysomes corresponds to that calculated for a monocistronic messenger RNA for the subunit of this enzyme, which has a molecular weight of 42,000. The application of immunochemical techniques to identification of templates for synthesis of an enzyme in embryonic cells that constitutes less than 1% of the total cellular proteins indicates the usefulness of this method for detailed studies on regulation of other quantitatively minor products significant in cell differentiation.


Biotransformation of drugs used in anesthesia.


Hepatic microsomal enzyme induction.

Hepatic microsomal enzyme induction.


Effect of anesthetics on mitochondrial function.


Anesthesia and hepatic metabolism: current concepts of carbohydrate homeostasis.


Inhalation anesthetics and myocardial metabolism: possible mechanisms of functional effects.


The dental health of five-year-old children of different ethnic origins resident in an inner Birmingham area and a nearby borough.


Antibodies to teichoic acids in man.

Antibodies to teichoic acids in man.


Alterations in enzyme activity and hyperexcitability in the chronic undercut cerebral cortex of monkey.


Papaver bracteatum Lindl.--a new plant source of opiates.


The central and peripheral activities of anti-acetylcholine drugs. Some concepts of practical relevance.


Intestinal absorption, intestinal distribution, and excretion of (14C) labelled hyoscine N-butylbromide (butylscopolamine) in the rat.


Serum and blood concentration of sodium cephalexin in man given single intramuscular and intravenous injections.


Gas-liquid chromatographic separation of the optical isomers of some "ephedrines" and "pseudoephedrines".


Oscillatory testing of o-w emulsions containing mixed emulsifiers of the surfactant-long chain alcohol type: influence of surfactant chain length.


An examination of acid-base equilibria of 1,4-benzodiazepines by spectrophotometry.


The effect of pH and of temperature on the stability and bioactivity of nystatin and amphotericin B.


Anti-inflammatory activity in human plasma.


Effect of ergot drugs on central catecholamine neurons: evidence for a stimulation of central dopamine neurons.


Dopa reversal of hypoxia-induced disruption of the conditioned avoidance response.


Abnormal O-methylated dopamine metabolites: the endogenous toxins of schizophrenia or parkinsonism?


Blood-brain barrier to carbidopa (MK-486) and Ro 4-4602, peripheral dopa decarboxylase inhibitors.


A note on the effect of (+)- and (-)-amphetamine on lipid metabolism.


Salivary excretion of paracetamol in man.


Hyperthermia and the amphetamine aggregation phenomenon: absence of a causal relation.


The demethylation of methamphetamine by intestinal microflora.


In vitro metabolism of neostigmine and pyridostigmine.


The influence of starch and lactose on the release rates of drugs from hard gelatin capsules.


Polyarteritis in the dog: a case report.


Some contributions of molecular biology to the question of viral carcinogenesis.


Cell division and disseminated neoplastic disease in man: a review.


The employment of frozen sections in cancer diagnosis.


Modern trends in the prevention of cancer recurrence.


The follow-up of the cancer patient.

The follow-up of the cancer patient.


Certain aspects of cancer control and the treatment of malignant tumors in the USSR.


The TNM-system.

The TNM-system.


Recent acquisitions in nuclear medicine for the detection of tumors.


Importance of the preoperative cancer evaluation.


Diagnostic methods for cerebral tumors. Present situation.


Diagnosis of spinal cord tumors. Present situation.


Plastic reconstruction after a disfiguring operation of the face and neck.


The combination of radiation and surgery in oropharynx and laryngopharynx squamous cell carcinomas.


The environmental biology of cancer of the stomach and large intestine.


The treatment of malignant tumors of the salivary glands at the Gustave-Roussy institute.


Treatment of cancer of the thyroid gland.


Surgical treatment of myasthenia.

Surgical treatment of myasthenia.


Thymomas, tumors of the thymic site, and the para-neoplastic immunological syndromes associated with them.


The surgical treatment of primary bronchopulmonary cancer.


Endothoracic neurogenic tumors in children and adults and their endocrine activity.


Morphological precursors of cancer in experimental and human pathology and their clinical significance.


Pulmonary coin lesions and metastases.

Pulmonary coin lesions and metastases.


Metabolically active tumors of the pancreas and the duodenum.


Primary cancers of the gastric stump after resection of the stomach in the treatment of ulcers. 3 cures lasting more than 7 years.


The treatment of gastro-intestinal polyps.


Chronic ulcero-hemorrhagic rectocolitis and cancer of the large intestine. Prophylactic panproctocolectomy.


The results of radical surgery for cancer of the extremities.


Treatment of pathological fractures.

Treatment of pathological fractures.


Modern treatment of bone sarcomas, indications and results.


Balance-sheet of breast cancer endocrine surgery.


Critical evaluation of methods of destroying the pituitary gland in the treatment of cancer.


Radioactive isotopes in the treatment of cancer.


Regional perfusion for cancer of the extremities.


Modern approaches to experimental cancer research. Roles of viruses and of cigarette smoke in carcinogenesis.


The treatment of hepatic metastases by long-term chemotherapeutic infusions.


Properties of halophil nicotinamide-adenine dinucleotide phosphate-specific isocitrate dehydrogenase. True Michaelis constants, reaction mechanisms and molecular weights. True values of Michaelis constants of the NADP(+)-specific isocitrate dehydrogenase from Halobacterium salinarium were not very different from those of the apparent constants reported by Aitken et al. (1970). The true constants were affected by salt in a similar manner to that of the apparent constants obtained with NADP(+) at fixed concentrations of 1.0-0.2mm and threo-d(s)-(+)-isocitrate at fixed concentrations of 2.0-0.125mm. The response of apparent V(max.) to salt concentration was highly dependent on fixed substrate concentration in solutions of sodium chloride but much less so in solutions of potassium chloride. At several levels the results emphasize the difficulty of generalizing about the salt relations of a halophil enzyme without adequate attention to substrate concentration. The enzyme has at least two different reaction mechanisms depending on salt concentration. In its ;physiological' form (i.e. in 1.0m-potassium chloride), and also in 1.0m-sodium chloride, the reaction mechanism is ordered with NADP(+) the first substrate added and NADPH the last product released. In 0.25m-sodium chloride, however, the mechanism is different and is probably non-sequential. In 4.0m-sodium chloride with low concentrations of either fixed substrate, there was evidence of a co-operative action of the variable substrate. The evidence suggests that salt participates in the reaction mechanism in two ways: one is the reversible addition to the enzyme in a manner analogous to that of a substrate; the other is dead-end complex-formation. The relative contributions of these two types of reaction determine whether salt activates or inhibits the enzyme. In addition, the inhibition caused by high concentrations of sodium chloride is more complex than the corresponding inhibition by potassium chloride. Gel-filtration experiments indicated that at very low salt concentrations the enzyme has an apparent molecular weight of about 70800. In ;physiological' concentrations of potassium chloride the enzyme appears to be a dimer (mol.wt. 122000-135000) and, in 1.0-4.0m-sodium chloride, it behaves as a trimer or tetramer (mol.wt. 224000-251000). A preliminary method of purifying the enzyme is described.


The characterization of two reduced nicotinamide-adenine dinucleotide phosphate-linked aldehyde reductases from pig brain. 1. NADPH-linked aldehyde reductase from pig, ox and rat brain exhibits non-linear reciprocal plots when partially purified enzyme preparations are studied. 2. In pig brain this non-linearity is due to the presence of two distinct aldehyde reductases, which can be separated by DEAE-cellulose chromatography. 3. These two enzymes can be distinguished by several criteria, including pH optima, Michaelis constants for substrates and their inhibitor sensitivity. 4. The probable role of these enzymes in the metabolism of the aldehydes derived from the biogenic amines is discussed.


Metabolism of glutamine and glutamic acid by isolated perfused kidneys of normal and acidotic rats. 1. When isolated kidneys from fed rats were perfused with glutamine the rate of ammonia release at pH7.4 (110-360mumol/h per g dry wt.) was one to two times that of glutamine removal. Glucose formation from 5mm-glutamine was 16mumol/h per g. If kidneys were perfused with glutamine at pH7.1 (10-13mm-sodium bicarbonate) there was no increase in glutamine removal or in the formation of ammonia or glucose. 2. When isolated kidneys from fed rats were perfused with glutamate at pH7.4, glucose formation was 59mumol/h per g, glutamine formation was 182mumol/h per g and ammonia release was negligible. At pH7.1 glutamine synthesis was inhibited and formation of ammonia and glucose were increased. 3. In perfused kidneys from acidotic rats, which had received 1.5% (w/v) NH(4)Cl to drink for 7-10 days, gluconeogenesis from glutamine was enhanced (101mumol/h per g). Glutamine removal and ammonia formation were also increased, compared with the rates in perfused kidney from normal rats. The extra glutamine consumed was equivalent to the extra glucose formed. 4. When the kidney from the 7-10-day-acidotic rat was perfused with glutamate gluconeogenesis was increased (113mumol/h per g). Synthesis of glutamine was decreased, and ammonia release was approximately equal to the rate of glutamate removal. 5. The time-course of these metabolic alterations was investigated after the rapid induction of acidosis by infusion of 0.25m-HCl into the right side of the heart. The increase in gluconeogenesis from glutamine developed gradually over several hours. When kidneys from 6h-acidotic rats were perfused with glutamate, formation of glucose and glutamine were both rapid. 6. In acidotic rat kidneys perfused with glutamine, tissue concentrations of glutamate and glucose 6-phosphate were increased compared with those in control perfused kidneys from non-acidotic rats. 7. The results are discussed in terms of control of the renal metabolism of glutamine. In particular, it is suggested that in acidotic rats glucose formation is the major fate of the carbon of the extra glutamine utilized by the kidney, and that inhibition of glutamine synthetase could contribute to the increase in intracellular ammonia concentration in the kidney.


The effect of L-ascorbate on catecholamine biosynthesis. l-Ascorbate stimulates the enzymic hydroxylation of phenylalanine in vitro by recycling tetrahydrobiopterin, which reduces O(2) utilized in the reaction. It is suggested that ascorbate might have a similar function in vivo; this would explain the apparent regulation of tyrosine hydroxylase and tryptophan hydroxylase activities by this vitamin.


Stereoconfiguration of amino acids in peptides from a mycobacillin-synthesizing cell-free system. The stereoconfiguration of amino acids, as determined by treatment with L-amino acid oxidase, d-amino acid oxidase and l-glutamate decarboxylase (containing l-aspartate decarboxylase activity), in the peptides from a mycobacillin-synthesizing cell-free system is identical with that of the growing mycobacillin peptide chaid if its synthesis starts from l-proline and is interrupted at various points by amino acid deprivation.


Glutamate, GABA and the direct cortical response in the rat.


Polyarteritis nodosa of the gallbladder, presenting as acute cholecystitis.


Salicylazosulfapyridine in clinical practice.


Autoregulatory escape in the gut: a systems analysis.


Activation by chemical carcinogens of gamma-glutamyl transpeptidase in rat and mouse liver.


Biosynthesis of cholest-5-ene-3beta, 24-diol (cerebrosterol) by bovine cerebral cortical microsomes.


Catecholamines in fetal and newborn rat brain.


Pilot studies on the clinical use of the short-lived isotope fluorine-18.


Muscle transplantation in normal and dystrophic animals.


Undescended testes.

Undescended testes.


The onic dependence of the strength and spontaneous relations of the potassium contracture induced in the heart of the frog Rana pipiens. 1. The tension generated by isolated frog atrial trabecules, during exposure to solutions containing a high potassium concentration, is not maintained but spontaneously relaxes. The final part of this relaxation can be fitted by a single exponential function.2. The recovery of the tension generating mechanisms following the spontaneous relaxation of a potassium contracture depends on the preceding membrane potential and the time since the last contracture.3. The rate of the exponential phase of the spontaneous relaxation is independent of the [K](o) and hence the membrane potential, the [Ca](o); and when the [Ca](o)/[Na](o) (2) ratio is maintained it is also independent of the [Na](o). This relaxation is not influenced by atropine or pronethalol.4. When sodium is totally excluded from the bathing medium the rate of relaxation of a later potassium contracture is much increased. It is argued that this change is due to a fall in the intracellular sodium concentration.5. The consequences of these results are discussed, and the hypothesis that is favoured would require that contraction is induced by a transient release of calcium into the sarcoplasm, probably triggered by a potential dependent, and probably also transient, influx of calcium through the cell membrane. Relaxation is supposed to occur when this activator-calcium is then removed by an intracellular relaxing system that resembles the sarcoplasmic reticulum of other muscles. What this intracellular structure might be, is also discussed.


Regional enteritis and growth retardation without intestinal symptoms.


Respiratory effects of acebutolol hydrochloride.


Study of pathogenesis of enteric Escherichia coli infection in model experiments on rabbits.


Adrenal corticosteroid effects upon rat brain mitochondrial metabolism.


The effect of androgenic hormones on the reduced nicotinamide adenine dinucleotide phosphate:delta-4-3-ketosteroid 5 alpha-oxidoreductase of rat ventral prostate.


Stimulation by insulin and glucagon of ornithine decarboxylase activity in perfused rat livers.


The place of some newer non-anaesthetic drugs in anaesthetic practice.


Renal ammoniagenesis and tissue glutamine, glutamine synthetase, and glutaminase I levels in potassium-deficient rats.


Avoidance conditioning in different strains of rats: neurochemical correlates.


Pigment production of Staphylococcus aureus PS 80 and its mutants.


Enterocine-typing of group D streptococci.


The temperature requirements of growth of some mycoplasmas.


Observations on experimental sporotrichosis of the muscle.


The distribution between gamma-glutamylhydrozamate synthetase and L-glutamine-hydroxylamine glutamyltransferase activities in rat tissues. Studies in vitro. Two common ways of measuring the potential for glutamine synthesis in a tissue are the rates of formation of gamma-glutamylhydroxamate either by synthesis from glutamate (the glutamylhydroxamate synthetase reaction) or by transfer from glutamine (the glutamyltransferase reaction); it has not been established, however, that either reaction is a specific measure of glutamine synthetase. By differential extraction of glutamylhydroxamate synthetase and glutamyltransferase activities from water homogenates of several rat tissues I obtained an extract, rich in glutamylhydroxamate synthetase activity but nearly devoid of glutamyltransferase activity, and a fraction, solubilized by deoxycholate from the pellet, which contained virtually no glutamylhydroxamate synthetase activity but most of the original glutamyltransferase activity. Synthesis of glutamine, quantitatively similar to the gamma-glutamylhydroxamate formed by glutamylhydroxamate synthetase, is catalysed in the water extract but not in the particulate fraction. gamma-Glutamylhydroxamate formation by glutamylhydroxamate synthetase and glutamyltransferase shows discrepant substrate and metal specificities and can be differentially inhibited by l-methionine sulphoximine, phosphate and adenine nucleotides. The concordance between the formation of glutamine and gamma-glutamylhydroxamate by glutamylhydroxamate synthetase but not by glutamyltransferase and the different solubilities of the glutamylhydroxamate synthetase and glutamyltransferase enzyme activities demonstrate that these two activities are not inextricably associated; they therefore cannot be catalysed exclusively by the same protein.


The distinction between gamma-glutamylhydroxamate synthetase and L-glutamine-hydroxylamine glutamyltransferase activities in rat tissues. The formation of gamma-glutamylhydroxamate by homogenates under optimum assay condition showed an inconstancy in the ratios of the enzyme activities utilizing l-glutamate and ATP (gamma-glutamylhydroxamate synthetase) and l-glutamine and ADP (l-glutamine-hydroxylamine glutamyltransferase) in a number of normal and neoplastic rat tissues. Although gamma-glutamylhydroxamate synthetase activities in adult livers and kidneys were identical in males and females, l-glutamine-hydroxylamine glutamyltransferase activities in the organs of females were significantly lower. The developmental formations of the two activities in liver, kidney, brain and muscle were not simultaneous. The l-glutamine-hydroxylamine glutamyltransferase activity in foetal liver or neonatal kidney could be prematurely evoked by thyroxine, but the gamma-glutamylhydroxamate synthetase activity remained unchanged. Injections of cortisol also had dissimilar effects on the two activities in thymus and hepatomas. The discrepant tissue distribution, asynchronous developmental formation and differential response to several hormonal stimuli provide evidence in vivo that the two activities are not catalysed by the same protein.


Transmission of hepatitis B antigen.

Transmission of hepatitis B antigen.


Inhibition by nicotinic acid of hepatic steatosis and alcohol dehydrogenase in ethanol-treated rats.


Studies on peroxisomes. IV. Intracellular localization of NADH2-glyoxylate reductase in rat liver.


Effect of ethanol on D-amino acid oxidase.


Structure and function of D-amino acid oxidase. V. Effect of urea on the structure of the free enzyme and its benzoate complex.


Hepatitis in the aged.

Hepatitis in the aged.


Structure and function of D-amino acid oxidase. VI. Relation between the quaternary structure and the catalytic activity.


Kinetics of interconversion of sulphamethoxydiazine crystal forms.


Effects of solubilization on drug diffusion.


Effect of chronically administered L-dopa on dopa 5HTP decarboxylase and tyrosine and tryptophan hydroxylases in cat brain.


A two phase series model for the transport of steroids across the fully hydrated stratum corneum.


The interaction of some oral contraceptive steroids with lipid monolayers and with Erythrocytes.


Antihistamine-monoamine oxidase inhibitor interaction in rabbits.


Homologues of benzilylcholine mustard.

Homologues of benzilylcholine mustard.


The effect of chlorthalidone on blood sugar and glucose tolerance in the rat.


Inotropic effects of atropine on ureteral muscle.


Investigations of the biosynthesis of novobiocin.


Linearization of dissolution rate curves by the Weibull distribution.


The effect of some oral contraceptive steroids on platelet electrophoretic mobility in vitro.


Urea increases serum albumin binding of drugs.


Absorption of iodipamide from the biliary system of the rabbit.


The antipyretic effect of alpha-methyldopa in experimental fever.


Monoamine metabolites in aqueous humour.


Possible role of gamma-aminobutyric acid in paraoxon-induced convulsions.


Interaction between sodium metabisulphite and PMN.


Decreased conversion of tyrosine to catecholamines in the brain of rats treated with p-chlorophenylalanine.


The action of vasoactive drugs on longitudinal and circular muscle of dog mesenteric vein.


Comparative convulsant activity of various penicillins after intracerebral injection in mice.


Metabolism and excretion of diethylpropion in man under acidic urine conditions.


Continuous shear, viscoelastic and spreading properties of a new topical vehicle, FAPG base.


Adsorption of benzoic acid on sulphadimidine: suppressive effect of some hydrophilic polymers.


Competitive binding of two drugs for a single binding site on albumin: a circular dichroic study.


Mass spectrometry in the analysis of steroid drugs and their metabolites: electron-impact-induced fragmentation of ring D.


The extraction and estimation of the cannabinoids in Cannabis sativa L. and its products.


Steryl esters of Digitalis purpurea L. herb.


Some observations on the pulmonary artery of the guinea-pig.


Catecholamine concentrations in discrete areas of the rat brain after the pre- and neonatal administration of phencyclidine and imipramine.


On the "quinidine-like action" of some antiarrhythmic agents.


Selective inhibition of angiotension-induced contractions of smooth muscle by indomethacin.


On the interaction between pimozide and alpha-methyltyrosine.


Blocking effect of alpha-methyltyrosine on amphetamine based reinforcement.


Penetration of 5-hydroxyindoleacetic acid across the blood-cerebrospinal fluid barrier.


Determination of tolbutamide and chlorpropamide in biological fluids.


Fluorometric determination of 3-methoxytyramine and 3-methoxy-4-hydroxyphenylethanol.


Chlorpromazine "hydroxylamines" in red blood cells as major metabolites of chlorpromazine in man.


N-ethyl-alpha-methyl-alpha-(m-trifluoromethylbenzyl)nitrone--the major in vitro metabolic product of fenfluramine in guinea-pig liver microsomal fractions.


The central action of antihypertensive drugs, mediated via central alpha-receptors.


Effects of adrenalectomy and of hypophysectomy on the development of sound-withdrawal hypertension.


Enhancement of serum renin activity by exercise in the rat.


Some typical recent cases in negligence.


Effectiveness of sectral (M & B 17803A) as a beta-blocking agent in man.


Purification of sea nettle nematocyst toxins by gel diffusion.


The role of the general practitioner in society: a radio talk given by Professor M.A. De Kock, Chairman of the Organizing Committee of the 49th Medical Congress to be held in Cape Town on 23-27 July 1973.


Interaction of the hemolysin of Chironex fleckeri tentacle extracts with lipid monolayers.


The intensity of anxiety in relation to the formation and amelioration of psychiatric symptoms.


Cost-benefit analysis of a hospital and university based prevocational program.


The effect of prolonged treatment with oxyfedrine on intracellular potentials and on other features of cardiac function in rabbits and guinea-pigs. 1. Previous work in acute experiments has shown that the main pharmacological action of oxyfedrine is stimulation of beta-adrenoceptors, yet there have been clinical reports that the drug is beneficial in the treatment of angina pectoris.2. In the present experiments rabbits and guinea-pigs were treated for several weeks with daily i.p. injections of oxyfedrine.3. A daily dosage of 15 mg/kg oxyfedrine had no effect on growth rate for 4 weeks, but thereafter the growth rate of treated animals fell below that of controls.4. The heart weights of the treated animals, expressed as a percentage of body weight, were significantly lower than those of controls.5. Measurement of intracellular potentials in hearts taken from treated rabbits showed that the main effects were a reduction in the maximum rate of depolarization and a prolongation of the plateau of the action potential.6. Guinea-pigs treated for 6 weeks with 15 mg/kg oxyfedrine daily i.p. were protected to some extent from the toxic effect of ouabain infused intravenously.


A comparison of the effects of chemical sympathectomy by 6-hydroxydopamine in newborn and adult rats. 1. The effects of chemical sympathectomy with 6-hydroxydopamine (6-OHDA) on the cardiovascular system of the rat were compared in, (a) 10-week-old rats treated during the first 14 days after birth with 150 mug/g subcutaneously, and (b) adult rats injected intravenously with 2 x 50 mg/kg on day 1 and 2 x 100 mg/kg on day 7 and the experiments performed on day 8.2. Intravenous administration of 6-OHDA to adult rats almost completely abolished the pressor responses to stimulation of the entire sympathetic outflow in the pithed rat, the contractions of the lower eyelid to stimulation of the cervical sympathetic trunk and the vasconstrictor responses produced by periarterial nerve stimulation of the isolated renal artery preparation. Pressor responses to physostigmine and to tyramine were markedly reduced or abolished in anaesthetized and pithed rat preparations, respectively.3. In corresponding experiments, 10-week-old rats treated as newborns with 6-OHDA showed a marked reduction in the stimulation-induced pressor responses and contractions of the lower eyelid, but completely normal vasoconstrictor responses to periarterial nerve stimulation of the isolated perfused renal artery were obtained. The pressor responses to physostigmine were slightly reduced but the tyramine responses were unchanged.4. Treatment with 6-OHDA at birth caused an almost complete and long-lasting noradrenaline depletion in the heart, spleen, salivary glands and ileum but only a partial depletion in the mesentery from 10-week-old rats. These low noradrenaline levels showed no recovery in rats up to an age of 4 months. The tyrosine hydroxylase activity in both the cervical and stellate ganglia from 10-week-old rats was markedly reduced by treatment with 6-OHDA after birth.5. Injections of 6-OHDA after birth produce an almost complete and permanent sympathectomy of various adrenergically innervated organs in the rat. The vascular system represents a major exception, exhibiting a surprisingly high resistance to this type of chemical adrenergic denervation.


Selective blockade of beta-adrenoceptors by 1-(p-allylphenoxy)-3-isopropylamino-2 propanol hydrochloride (h 64-52) in the anaesthetized dog. 1. The beta-adrenoceptor blocking properties of 1-(p-allylphenoxy)-3-isopropylamino-2-propanol hydrochloride (H 64/52) on the cardiovascular and bronchomotor responses to isoprenaline have been determined in the anaesthetized dog.2. H 64/52 selectively blocked the isoprenaline-induced increase in heart rate, contractile force and reduction in airway pressure in the same dose range.3. H 64/52 produced a minimal blockade of vascular beta-adrenoceptors which was apparent only when the isoprenaline-induced fall in diastolic pressure was considered. No significant vascular beta-adrenoceptor blockade with H 64/52 could be shown with the femoral arterial flow or hind-limb perfusion studies.4. These results further support the hypothesis that several different beta-adrenoceptor subtypes exist in the dog. The beta-adrenoceptors subserving cardiac stimulation and bronchodilation appear to be similar and both differ from the beta-adrenoceptor subserving vasodilatation.


Inhibition by gamma-hydroxybutyrate of chlorpromazine-induced increase in homovanillic acid. 1. gamma-Hydroxybutyrate and its lactone precursor, gamma-butyrolactone, when administered in anaesthetic doses block the increase in the cerebral concentration of homovanillic acid normally observed after administration of chlorpromazine or haloperidol.2. Other hypnotics such as glutethimide, urethane, chloralose, chloral hydrate and thiopentone do not have this ability even when administered in anaesthetic doses.3. The ability of gamma-hydroxybutyrate to block the neuroleptic-induced increase in cerebral homovanillic acid is not due to a reduction in body temperature since a similar effect is observed in rats treated under conditions (32 degrees C) in which body temperature remains normal.4. Possible mechanisms for this action of gamma-hydroxybutyrate are discussed.


Caries experience of 11-17-year-old children. A mixed longitudinal epidemiological study.


Venomous animal injuries.

Venomous animal injuries.


In vitro studies in Tubularia: a morphological role for digestive cells.


Drug-induced changes of striatal cholinergic and dopaminergic functions in rats with spreading depression.


Effect of ethanol on the hydroxylation of tyrosine and tryptophan in rat brain in vivo.


Some actions of 4,17a-dimethyl-4,17a-diaza-D-homo-5alpha-androstane dimethiodide (HS-342), a new neuromuscular blocking drug.


Pharmacology of diferuloyl methane (curcumin), a non-steroidal anti-inflammatory agent.


Plasma half-lives, plasma metabolites and anticoagulant efficacies of the enantiomers of warfarin in man.


Plasma protein binding of 7-hydroxy- 1-tetrahydrocannabinol: an active  1-tetrahydrocannabinol metabolite.


Micromethod for the determination of bupivacaine in maternal and foetal blood during obstetric analgesia.


Kinetics of hydrolysis of polyoxyethylene (20) sorbitan fatty acid ester surfactants.


Release of dipicolinic acid and calcium and activation of Bacillus stearothermophilus spores as a function of time, temperature and pH.


Preliminary observations on the uptake of catecholamines in synaptosomes of opiate-treated animals.


Two cannabidiol metabolites formed by rat liver.


The uptake and metabolic fate of cannabinoids in rat brains.


Antagonistic actions of alpha-methylnoradrenaline derived from alpha-methyldopa.


Biphasic action of a 5-hydroxytryptamine inhibitor on fenfluramine-induced anorexia.


Cardiovascular effects of 5-hydroxytryptophan in anaesthetized dogs.


Potentiation of leptazol seizures by 6-hydroxydopamine.


Effects of dihydroergotamine on glucose and galactose tolerance in the rabbit.


On the use of ethanol as a scavenger in storing labelled dopa and 5-hydroxytryptamine in aqueous solutions.


Effects of desipramine and reserpine on 'free' and 'bound' acetylcholine in rat brain.


Antibacterial activity of phenylmercuric nitrate in zinc sulphate and adrenaline eye drops B.P.C. 1968.


The preferred conformation of cyclophosphamide.


Investigations into the biosynthesis of novobiocin.


Nutrition and the body's defense mechanism.


Marijuana induced state-dependent verbal learning.


Malonyl-CoA and fatty acid synthesis.

Malonyl-CoA and fatty acid synthesis.


Current concepts of hemostasis. Progress report on platelet aggregation.


Mesenteric circulation in cardiogenic shock with and without alpha-receptor blockade.


Presidential address: "Future shock and the surgeon".


Reflex beta-adrenergic vasodilatation in the cat.


Effects and mechanisms of action of dopamine on regional hemodynamics in the conscious dog.


A longitudinal clinical study of oral cleanliness and dental caries in school children.


Inhibition of mitochondrial electron-transport systems by nor-isoguaiacin.


Feeding and aggressive behavior evoked by hypothalamic stimulation in a cichlid fish.


Contrasting twitch and latency relaxation in skeletal muscle.


Oxygen affinity of avian hemoglobins.

Oxygen affinity of avian hemoglobins.


Erythrocyte sodium and potassium levels in normal and anemic mice.


Renal adaptations to macro- and microhabitats in the family Cricetidae.


The circannual variations of thyroid activity in the woodchuck (Marmota monax).


The balance of cations in the plasma of vertebrates and its significance in relation to the properties of cell membranes.


The suitability of Melanoplus differentialis for electrophysiological investigation based on hemolymph ionic and anatomical studies.


Effects of fasting on the blood chemistry of the rattlesnake, Crotalus atrox.


Reversal of gamma-aminobutyric acid inhibition by carbon dioxide.


Protein synthesis in Fundulus heteroclitus muscle.


Thermal patterns in the heating and cooling of Iguana iguana and Ctenosaura hemilopha.


The relationship between cell sizes, respiration rates and survival of amphibian tissues in long-term organ cultures.


Histone fractions in chicken bursa of fabricius and thymus.


Depression of gustatory sweet response by alloxan.


Effects of thermal acclimation on physiological responses to handling stress, cortisol and aldosterone injections in the goldfish, Carassius auratus.


Metabolite changes in the neonate fowl in response to cold stress.


Intestinal pseudo-obstruction.

Intestinal pseudo-obstruction.


Spectral properties of glutamate dehydrogenase. Complexes involving substrate analogues.


Genotype-environmental interactions in Schizophyllum commune. 3. The relationship between mean expression and sensitivity to change in environment.


Rat adrenal 5alpha-reductase activity and the effect of oestradiol, testosterone propionate and adrenocorticotrophin in intact and gonadectomized rats.


The adrenals and gonads of congenitally thymusless nude mice.


Further studies on the mechanism of antipsychotic action: potentiation by alpha-methyltyrosine of thioridazine effects in chronic schizophrenics.


In-vivo measurements of tryptophan and tyrosine hydroxylase activities in mouse brain.


Torsion of the previously operated testicle.


Management of inguinal lymph nodes in patients with testicular tumors following orchiopexy, inguinal or scrotal operations.


Pharmacological studies of the therapy of shock.


Structure-function relationships in lactate dehydrogenase. The binding of coenzyme and substrate are considered in relation to the known primary and tertiary structure of lactate dehydrogenase (EC 1.1.1.27). The adenine binds in a hydrophobic crevice, and the two coenzyme phosphates are oriented by interactions with the protein. The positively charged guanidinium group of arginine 101 then folds over the negatively charged phosphates, collapsing the loop region over the active center and positioning the unreactive B side of the nicotinamide in a hydrophobic protein environment. Collapse of the loop also introduces various charged groups into the vicinity of the substrate binding site. The substrate is situated between histidine 195 and the C4 position on the nicotinamide ring, and is partially oriented by interactions between its carboxyl group and arginine 171. The spatial arrangements of these groups may provide the specificity for the L-isomer of lactate.


Methaqualone, methyprylon, and glutethimide clearance by the ACAC microcapsule artificial kidney: in vitro and in patients with acute intoxication.


Factitious hypoglycemia.

Factitious hypoglycemia.


The pathways of nitrogen fixation.

The pathways of nitrogen fixation.


Angina pectoris induced by dumping syndrome in idiopathic hypertrophic subaortic stenosis.


Haemodynamic and electrocardiographic effects of practolol during upright exercise in coronary heart disease.


Determinants of cardiac noradrenaline depletion in human congestive failure.


Functional monitoring of spinal cord activity during spinal surgery.


Phenobarbital liver microsomal induction in MHV-3 viral hepatitis of the mouse.


Effect of adrenergic amines on the membrane potential of guinea-pig liver parenchymal cells in short term tissue culture.


Nitrite reductase-deficient mutants of Escherichia coli K12.


Role of mosquitoes in transmission of hepatitis B antigen.


Cells involved in the graft-versus-host reaction in vitro.


Smallpox vaccination following childhood postvaccinal encephalitis.


Inhibiting effects of antihistamine and antiserotonin drugs on the bone marrow reaction caused by endotoxin.


Pharmacological activity of some neuro-transmitters in the isolated thoracic duct of dogs.


Pediatric surgery. 3.

Pediatric surgery. 3.


Preliminary studies of nematocysts from the jellyfish Stomolophus meleagris.


Surface properties of the hemolytic fraction derived from tentacle extracts of Chironex fleckeri.


Dopa decarboxylase in medullary carcinoma of the thyroid.


Toxicity of the zoanthid Palythoa tuberculosa.


Fatal intoxication of rabbits, sheep and monkeys by the venom of the sea wasp (Chironex fleckeri).


Fatty acid synthesis in the brown fat and liver of foetal and newborn rabbits. The postnatal fall in fatty acid synthesis in the liver and brown fat of newborn rabbits is accompanied in both tissues by a decrease in the capacities of the enzymes of fatty acid synthesis and an apparent increase in the degree of inhibition of one or more of these enzymes.


Effects of infection with Diplococcus pneumoniae on synthesis of ribonucleic acids in rat liver. Rats infected with virulent Diplococcus pneumoniae developed a significant increase in the rate of incorporation of labelled orotate into hepatic RNA when compared with pair-fed controls inoculated with heat-killed organisms. The finding was readily detected in rats raised on either a low-protein diet (6% casein) or a diet containing adequate amounts of protein (18% casein). The increase in hepatic RNA synthesis was observed by 12h and was maximal by 16-20h after inoculation with the D. pneumoniae. Most of the infection-related increase in RNA synthesis was associated with the bound ribosomal RNA fraction of the liver. A small but less significant increase was observed in the synthesis of free ribosomal RNA. The increased synthesis of RNA in the liver of infected rats resulted in a marked elevation of the liver RNA/DNA ratio, the major increase being observed in concentration of bound ribosomal RNA fraction. When followed sequentially, the infection-related increase in synthesis of hepatic RNA was preceded by a flux of amino acids into liver and was followed by elevated synthesis rates of serum globulin proteins. These findings suggest that the infectious process was able to regulate hepatic RNA synthesis by altering the rate of transcription of new RNA. This mechanism was stimulated even in rats that had been severely depleted of body protein and amino acids by feeding them on a low-protein diet. The infection-related stimulation of liver RNA and protein synthesis thus appeared to take place at the expense of other body proteins.


Hyperproduction mutation in the dihydrofolate reductase gene of Diplococcus pneumoniae: some effects on synthesis correlate with the presence of a modified enzyme form.


The effects of practolol on angina pectoris induced by atrial pacing.


Studies on the enzymology of purified preparations of brush border from rabbit kidney. 1. A method for the preparation of brush border from rabbit kidneys is described. Contamination by other organelles was checked by electron microscopy and by the assay of marker enzymes and was low. 2. Seven enzymes, all hydrolases, were substantially enriched in the brush-border preparation and are considered to be primarily located in this structure. They are: alkaline phosphatase, maltase, trehalase, aminopeptidase A, aminopeptidase M, gamma-glutamyl transpeptidase and a neutral peptidase assayed by its ability to hydrolyse [(125)I]iodoinsulin B chain. 3. Adenosine triphosphatases were also present in the preparation, but showed lower enrichments. 4. Alkaline phosphatase was the most active phosphatase present in the preparation. The weak hydrolysis of AMP may well have been due to this enzyme rather than a specific 5'-nucleotidase. 5. The two disaccharidases in brush border were distinguished by the relative heat-stability of trehalase compared with that of maltase. 6. The individuality of the four peptidases was established by several means. The neutral peptidase and aminopeptidase M, both of which can attack insulin B chain, differed not only in response to inhibitors and activators but also in the inhibitory effect of a guinea-pig antiserum raised to rabbit aminopeptidase M. This antiserum inhibited both the purified and the brush-border activities of aminopeptidase M. The neutral peptidase and gamma-glutamyl transpeptidase were unaffected but aminopeptidase A was weakly inhibited. The characteristic responses to Ca(2+) and serine with borate served to distinguish aminopeptidase A and gamma-glutamyl transpeptidase from other peptidases. 7. No dipeptidases, tripeptidases or carboxypeptidases were identified as brush-border enzymes. 8. Incubation of brush border with papain released almost all the aminopeptidase M activity but only about half the activities of maltase, gamma-glutamyl transpeptidase and aminopeptidase A. No release of alkaline phosphatase, trehalase or the neutral peptidase was observed.


CNS effects of 1-isopropylamino-3-(4-indanoxy)-2-propanol hydrochloride (USVC-6524).


The transfer of salicylic acid across a cellophane membrane from micellar solutions of polysorbates 20 and 80.


A note on the solubilization of preservative mixtures by cetomacrogol.


The interaction of penicillins with phospholipids.


Use of substituent constants in structure-activity relations and the importance of the choice of standard state.


Behavioural and biochemical interaction between caffeine and L-dopa.


The excitation of neurons by noradrenaline.


The activity of beta-adrenoceptor blocking agents in protecting mice from the cardiotoxic effects of ouabain.


The effect of oral administration of various sugars on blood ethanol concentrations in man.


Differential effects of amphetamine, fenfluramine and norfenfluramine stereoisomers on the increase of striatum homovanillic acid in rats.


Gas chromatographic determination of ethosuximide and phensuximide in plasma and urine of man.


The identification, properties and analysis of N-hydroxyamphetamine--a metabolite of amphetamine.


Metabolism of amitriptyline, nortriptyline, imipramine and desipramine to yield hydroxylamines.


Iron deficiency anaemia and drug metabolism.


Iminostilbene--a metabolite of carbamazepine isolated from rat urine.


Interaction between DDT and lecithin in spread monolayers.


Preparation of  9-tetrahydrocannabinol for intravenous injection.


Glutarimide antibiotics and drug metabolism in the rat.


An intravenous formulation of  9-tetrahydrocannabinol using a non-ionic surfactant.


Effect of clozapine on the turnover of dopamine in the corpus striatum and in the limbic system.


The effect of trivastal, haloperidol and dibutyryl cyclic AMP on (14C)dopamine synthesis in rat striatum.


Pharmacological studies on a possible role of central noradrenaline neurons in respiratory control.


Dissociation of alpha adrenergic receptor protection from inhibition of H-phenoxybenlamine binding in vascular tissue.


Comparison of the beta receptor blocking properties of two analogs of dichloroisoproterenol (DCI).


The regulation of pineal serotonin by a beta adrenergic receptor.


The selective beta receptor blocking properties of DL-1-(2-acetyl-4-n-butyramidophenoxy)-2-hydroxy-3-isopropylaminopropane HC1 (M & B 17803-A) in the anesthetized dog.


Dromotropic effects of optical isomers of beta blockers on the cross-circulated canine-atrioventricular node preparation.


Alcohol withdrawal reactions in mice: effects of drugs that modify neurotransmission.


Effect of local anesthetics on pharmacologic receptor systems of smooth muscle.


Cytochemical demonstration and localization of adenyl cyclase in skeletal and cardiac muscle.


Regulation of cyclic AMP levels in brain tissue.


Psychopharmacological agents and the cyclic AMP system of rat brain.


Protection against secondary syndrome by facilitating immune sera.


School fluoridation for the prevention of dental caries.


A sentence completion test assessing safety and esteem motives.


Alternatives to fluoridation.

Alternatives to fluoridation.


Replicated empirical correlated of the MMPI 8-9-9-8 code type.


History of fluoride, dental fluorosis, and concentrations of fluoride in surface layer of enamel of caries-free naval recruits.


The relationship of frequency of toothbrushing, oral hygiene, gingival health, and caries-experience in school children.


Adverse reactions during salicylazosulfapyridine therapy and the relation with drug metabolism and acetylator phenotype.


Therapeutic implications of the intestinal microflora.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 38-1973.


The World Health Organization. 25 years of endeavour.


Positive-contrast peritoneography and heriography.


One-electron and two-electron transfer mechanisms in enzymic oxidation-reduction reactions.


Cardiac histological and serum-biochemical changes produced by various adrenergic stimulant drugs in the rat.


Effects of oxyfedrine on isolated portal vein and other smooth muscles. 1. Oxyfedrine (0.01-1.0 mug/ml), inhibited spontaneous myogenic activity in rat isolated portal vein and carbachol-induced contractions of rat isolated uterus, and relaxed the rabbit duodenum and the guinea-pig tracheal chain preparation. These actions were prevented by the beta-adrenoceptor blocking drug alprenolol. Oxyfedrine was a relatively weak beta-adrenoceptor stimulant (10-100 times less active than isoprenaline) but its actions were more prolonged.2. In the same concentrations, oxyfedrine reduced or prevented the inhibition of myogenic activity of the rat portal vein induced by isoprenaline and by repeated doses of oxyfedrine itself, acting as a partial agonist at beta-adrenoceptor sites.3. Oxyfedrine, 1-12 mug/ml increased myogenic activity in the rat portal vein. This effect was not due to direct or indirect stimulation of alpha-adrenoceptors (because it was unaffected by phentolamine) or to potentiation of acetylcholine or 5-hydroxytryptamine.4. Oxyfedrine (>20 mug/ml) inhibited spontaneous myogenic activity in the portal vein and relaxed the saphenous vein contracted with noradrenaline. This spasmolytic effect of the drug was not due to beta-adrenoceptor stimulation or to inhibition of phosphodiesterase since it was unaffected by alprenolol and by concentrations of imidazole which antagonized the effects of the active phosphodiesterase inhibitor, papaverine. In the portal vein this effect of oxyfedrine was similar to that of the calcium inhibitor iproveratril; some of the effects of oxyfedrine on venous smooth muscle may be mediated through effects on calcium transport.


Effects of drugs and of electrical stimulation on the muscularis mucosae of rabbit large intestine. 1. The muscularis mucosae of the rabbit large intestine shows spontaneous rhythmic contractions with an average frequency of about 11/minute.2. The muscularis mucosae responds to acetylcholine, adrenaline and noradrenaline in a manner similar to that of whole segments of terminal large intestine.3. The muscularis mucosae would seem to have a cholinergic motor innervation.


Attenuation of amphetamine-induced motor stimulation and stereotypy by 6-hydroxydopamine in the rat. 1. In accord with previous reports, intraventricular administration of 6-hydroxydopamine (250 mug) to rats did not influence spontaneous locomotor activity. Neither was the stereotyped behaviour seen after high doses of (+)-amphetamine (5 mg/kg) changed by this treatment. Increases in motor activity induced by (+)-amphetamine (0.5 and 1.0 mg/kg) were significantly reduced after 6-hydroxydopamine.2. When 6-hydroxydopamine (250 mug) was administered to tranylcypromine (5 mg/kg) pretreated animals, spontaneous activity was significantly reduced. The stimulant effects of (+)-amphetamine (0.5 and 1.0 mg/kg) were completely abolished and amphetamine stereotypy (5.0 mg/kg) was absent or reduced after this treatment.3. Bilateral injections of 6-hydroxydopamine (10 mug) into the substantia nigra abolished the more pronounced features of amphetamine stereotypy. However, although significantly reduced, amphetamine-induced locomotor stimulation was observed in these animals. Spontaneous activity was also reduced.4. These observations suggest that dopaminergic nigro-striatal neurones mediate some of the stimulant effects of amphetamine as well as being of critical importance in amphetamine-induced stereotypy. However, other catecholaminergic neurones also appear to be involved in amphetamine motor stimulation. The results are consistent with the view that amphetamine exerts its behavioural effects indirectly through its action on brain catecholamines.


Dermatological side effects with psychotropics.


Immunological induction of DNA synthesis in mouse peritoneal macrophages. An expression of cell-mediated-immunity.


Changes in guinea-pig liver heparin and histamine during anaphylaxis.


Immunochemical studies on function of NADH: hemeprotein oxidoreductase in electron transport system of chromatophores from Rhodospirillum rubrum.


Adenosine 3', 5'-monophosphate and histone phosphorylation during enzyme induction by glucagon in rat liver.


Immunological studies on function of NADH: quinone oxidoreductase in electron transport system of chromatophores from Rhodospirillum rubrum.


A beta-adrenergic receptor of the turkey erythrocyte. I. Binding of catecholamine and relationship to adenylate cyclase activity.


Effects of fatigue produced by isokinetic exercise on the communication ability of aphasic adults.


Lack of correlation between catecholamine effects on cyclic adenosine 3':5'-monophosphate and gluconeogenesis in isolated rat liver cells.


Enteritis associated with pneumococci.

Enteritis associated with pneumococci.


The facial-digital-genital (Aarskog) syndrome.


Measurement of personality traits: theory and technique.


Measurement of impairment and disability in dermatology.


The Pueblo incident. A comparison of factors related to coping with extreme stress.


Effects of vinblastine on catecholamine-biosynthetic enzymes in heart, sympathetic ganglion and adrenal glands of rats. 1. The effect of vinblastine on the activities of dopamine-beta-hydroxylase (DBH) in heart, superior cervical ganglion and adrenal glands of rats and tyrosine hydroxylase (TH) and phenylethanolamine-N-methyl transferase (PNMT) in adrenal glands was examined.2. In the superior cervical ganglion and heart, DBH activity decreased within hours, reached a minimum in 3 to 5 days and slowly returned towards normal over the next 2 weeks.3. There was an increase in the activities of TH, DBH and PNMT in adrenal glands which was prevented by adrenal denervation.4. When the same total dose of vinblastine was administered subdivided over a period of 5 days, enzyme activities in heart, superior cervical ganglion or adrenal glands remained unchanged.


Characterization of  -adrenoceptors in the myocardium. 1. In the driven rabbit left atrium alpha-adrenoceptors mediate the inotropic effect of phenylephrine and the prolongation of functional refractory period by sympathomimetic amines.2. These receptors are highly sensitive to blockade by phentolamine and phenoxybenzamine.3. Prolongation of functional refractory period is greater with the secondary amines, phenylephrine, adrenaline and epinine, than with the primary amines, norphenylephrine, noradrenaline and dopamine.


Some observations on the  -adrenoceptor agonist properties of the isomers of salbutamol. 1. The pharmacological activities of the optical isomers of salbutamol have been examined. (-)-Salbutamol was much more potent than (+)-salbutamol on beta-adrenoceptors.2. Both (-)- and (+)-salbutamol showed high selectivity for beta-adrenoceptors in bronchial muscle compared to cardiac muscle, in this way resembling racemic salbutamol.3. The use of isomeric activity ratio to detect differences between receptors was examined in the light of the results obtained with the isomers of salbutamol.


Oxidation of the capsular polysaccharide of pneumococcal type IV by periodate.


Pharmacological analysis of the acute inflammatory process induced in the rat's paw by local injection of carrageenin and by heating. 1. Local injection of carrageenin in the rat's paw produced oedema and leakage of dye which had been administered previously by the intravenous route. A net dissociation between both parameters was observed: while oedema developed slowly, maximal intensity being attained after 4-5 h, dye-leakage was maximum after 1 hour.2. Anti-inflammatory drugs such as acetylsalicylic acid and indomethacin were effective in reducing oedema and dye-leakage when given before the injection of carrageenin, but much less effective when given 30 or 60 min after carrageenin. Hexadimethrine bromide was effective in reducing dye-leakage also when given 1 h after the injection of carrageenin.3. Combined administration of benadryl and methysergide, before the injection of carrageenin caused only a slight reduction in oedema and dye-leakage.4. When the paws were heated (55 degrees C for 30 s) as a noxious stimulus the dissociation between maximal oedema and maximal dye-leakage was not observed, both phenomena running parallel. Pre-treatment of the animals with indomethacin did not afford any protection.5. These results suggest that the inflammatory reaction to mild stimuli (carrageenin in our experiments) develops through different phases: initially the increased vascular permeability involves extravasation of plasma proteins and that phase is followed by an increased permeability mainly to water. Stronger stimuli (heating in our experiments) produce an overlapping of both phases, probably by inflicting severe damage to the vascular bed of the affected area.6. The anti-inflammatory drugs employed affected chiefly the initial phase of the response.


The clinician's approach to antiarrhythmic therapy.


Antiarrhythmic agents. I. Mechanisms of action and clinical pharmacology.


The effects of anoxia on isozyme patterns and ultrastructure of cultured human fibroblasts.


Role of the tear film in contact lens wear.


Tyrosine-alpha-ketoglutarate aminotransferase activity in rat liver after spinal cord section.


Proximal contacts in posterior teeth and factors influencing interproximal caries.


Current status of propranolol hydrochloride (inderal).


Corticosteroids in the treatment of sunburn.


Stimulant action of methoxamine in the isolated atria of normal and 6-propyl-2-thiouracil-fed rats.


Studies on a new 1,5-benzothiazepine derivative (CRD-401). V. Antiarrhythmic actions.


Antagonistic activity of some phenothiazine derivatives against neurohumoral transmitters.


Use of hemodialysis in the treatment of quinidine poisoning.


Collagen-induced platelet aggregation: involement of an active glycopeptide fragment (alpha1-CB5). It is widely held that the tertiary structure of collagen is essential for induction of platelet aggregation. However, we have found that the purified alpha1 chain prepared from denatured chick skin collagen aggregates platelets. This activity appears to be confined to a distinct region of the molecule representing less than 4 percent of the length of the alpha1 chain. Of all of the cyanogen bromide peptides of the alpha1 chain tested, only one (alpha1-CB5) was active. This glycopeptide, devoid of any ordered tertiary structure, contains only 36 amino acids and one residue of O-alpha-D-glucopyranosyl-(1 --> 2)-O-beta-D-galactopyranosyloxy-(1 --> 5)-lysine (Glc-Gal-Hyl). Blocking experiments strongly suggest that the Glc-Gal-Hyl is one of the structural determinants involved in collagen-induced platelet aggregation.


The promotion of interest in the role of the physician associate as a potential career opportunity for nurses: an alternative strategy.


First International Symposium and Workshop on the Polymorphism of the Third Component of the Human Complement System. Bonn, June 26-28, 1972 at the Institute of Forensic Medicine of the University, Bonn, FRG.


Effects of a new cardioselective beta-adrenergic blocker (tolamolol) on exercise tolerance in patients with angina pectoris.


A modification of isocitrate and malate dehydrogenase assays for use in crude cell free extracts.


The cardiac effects of cocaine and certain antihistamines and antidepressants.


Action of  -adrenergic blocking drugs on the sympathetic centres and their interactions with the central sympatho-inhibitory effect of clonidine.


The degradation of L-histidine, imidazolyl-L-lactate and imidazolylpropionate by Pseudomonas testosteroni. 1. Imidazol-5-ylpropionate and imidazol-5-yl-lactate are degraded by Pseudomonas testosteroni via inducible pathways. 2. Growth on either compound as the sole source of carbon results in the induction of the enzymes for histidine catabolism. 3. The pathway of histidine degradation in this organism, a non-fluorescent Pseudomonad, is shown to be the same as that operating in Pseudomonas fluorescens and Pseudomonas putida. It consists of the successive formation of urocanate, imidazol-4-on-5-ylpropionate, N-formimino-l-glutamate, N-formyl-l-glutamate and glutamate. 4. Whole cells of P. testosteroni accumulate urocanate in the reaction mixture when incubated with imidazolylpropionate, but only after an adaptive lag period which is removed by previous growth on imidazolylpropionate as the source of carbon. 5. Imidazolyl-lactate is oxidized to imidazolylpyruvate, which then gives rise to histidine by specific transamination with l-glutamate. 6. Cells grown on histidine, urocanate or imidazolylpropionate are also able to degrade imidazolyllactate. 7. Mutants lacking urocanase are unable to grow on imidazolylpropionate, imidazolyl-lactate, histidine or urocanate. One with impaired histidase activity cannot utilize histidine or imidazolyl-lactate, but grows normally on imidazolylpropionate or urocanate. A mutant unable to grow on imidazolylpropionate is indistinguishable from the wild-type with respect to growth on histidine, imidazolyl-lactate or urocanate. 8. Thus it is established that imidazolyl-lactate is metabolized via histidine whereas imidazolylpropionate enters the histidine degradation pathway after conversion into urocanate.


The control of the enzymes degrading histidine and related imidazolyl derivates in Pseudomonas testosteroni. 1. The induction of the enzymes for the degradation of l-histidine, imidazolylpropionate and imidazolyl-l-lactate in Pseudomonas testosteroni was investigated. 2. The activities of histidine ammonia-lyase, histidine-2-oxoglutarate aminotransferase and urocanase are consistent with these enzymes being subject to co-ordinate control under most growth conditions. However, a further regulatory mechanism may be superimposed for histidase alone under conditions where degradation of histidine must take place for growth to occur. 3. Experiments with a urocanase(-) mutant show that urocanate is an inducer for the enzymes given above and also for N-formiminoglutamate hydrolyase and N-formylglutamate hydrolase. 4. N-Formiminoglutamate hydrolase and N-formylglutamate hydrolase are also induced by their substrates, and it is suggested that these two enzymes may be different gene products from those expressed in the presence of urocanate. 5. Induction of the enzyme system for the oxidation of imidazolylpropionate is dependent on exposure of cells to this compound.


Regulation by cytidine nucleotides of the acylation of sn-(14C)glycerol 3-phosphate. Regional and subcellular distribution of the enzymes responsible for phosphatidic acid synthesis de novo in the central nervous system of the rat. 1. The regional and subcellular distribution of the incorporation of sn-[(14)C]glycerol 3-phosphate into rat brain lipids in vitro was investigated and compared with the relative specific activity of various chemical and enzyme markers. The similarity between the subcellular distribution of this incorporation and of NADPH-cytochrome c reductase activity indicated that the synthesis of phosphatidic acid via this route correlated with the presence of endoplasmic reticulum. 2. Experiments in which various amounts of the microsomal fraction were added to fixed amounts of nuclear, myelin, nerve-ending and mitochondrial preparations clearly demonstrated that the endoplasmic-reticulum contamination of these fractions was entirely responsible for the incorporation of sn-[(14)C]glycerol 3-phosphate. 3. The presence of CMP or CTP inhibited the incorporation of sn-[(14)C]glycerol 3-phosphate into the whole homogenate. Similar effects were observed with individual fractions, except for the mitochondria. With the mitochondrial fraction the effect of these cytidine nucleotides varied with the preparation, stimulating in some preparations and inhibiting with other preparations. The presence of CDP-choline stimulated the incorporation into the whole homogenate and to a lesser extent into the subcellular fractions. 4. These results indicate that the various organelles of the central nervous system are more dependent on endoplasmic reticulum for the production of glycerolipids de novo than has previously been appreciated.


Insulin and the regulation of adipose tissue acetyl-coenzyme A carboxylase. Rat epididymal fat-pads were incubated for 30min with glucose (2mg/ml) in the presence or absence of insulin. A twofold or greater increase in acetyl-CoA carboxylase activity was observed in extracts from insulin-treated tissue provided that assays were performed rapidly after extraction. This effect of insulin was evident whether or not extracts were prepared with albumin, and was not noticeably diminished by the presence of citrate or albumin or both in the assay. Incubation of extracts before assay led to activation of acetyl-CoA carboxylase and a marked diminution in the insulin effect. The enzyme in extracts was very sensitive to reversible inhibition by palmitoyl-CoA even in the presence of albumin (10mg/ml); inhibition persisted on dilution of enzyme and inhibitor. It is suggested that the observed activation of acetyl-CoA carboxylase by insulin may reflect changes in enzyme activity in the fat-cell resulting from the reduction of long-chain fatty-acyl-CoA that occurs in the presence of insulin. Activation of the enzyme with loss of the insulin effect on incubation of the extracts may be due to the slow dissociation of long-chain fatty acyl-CoA from the enzyme.


The proton-translocating nicotinamide-adenine dinucleotide (phosphate) transhydrogenase of rat liver mitochondria. 1. The NAD(P) transhydrogenase activity of the soluble fraction of sonicated rat liver mitochondrial preparations was greater than the NAD-linked isocitrate dehydrogenase activity, and the NAD-linked and NADP-linked isocitrate dehydrogenase activities were not additive. The NAD-linked isocitrate dehydrogenase activity was destroyed by an endogenous autolytic system or by added nucleotide pyrophosphatase, and was restored by a catalytic amount of NADP. 2. We concluded that the isocitrate dehydrogenase of rat liver mitochondria was exclusively NADP-specific, and that the oxoglutarate/isocitrate couple could therefore be used unequivocally as redox reactant for NADP in experiments designed to operate only the NAD(P) transhydrogenase (or loop 0) segment of the respiratory chain in intact mitochondria. 3. During oxidation of isocitrate by acetoacetate in intact, anaerobic, mitochondria via the rhein-sensitive, but rotenone- and arsenite-insensitive, NAD(P) transhydrogenase, measurements of the rates of carbonyl cyanide p-trifluoromethoxyphenylhydrazone-sensitive and carbonyl cyanide p-trifluoromethoxyphenylhydrazone-insensitive pH change in the presence of various oxoglutarate/isocitrate concentration ratios gave an -->H(+)/2e(-) quotient of 1.94+/-0.12 for outward proton translocation by the NAD(P) transhydrogenase. 4. Measurements with a K(+)-sensitive electrode confirmed that the electrogenicity of the NAD(P) transhydrogenase reaction corresponded to the translocation of one positive charge per acid equivalent. 5. Sluggish reversal of the NAD(P) transhydrogenase reaction resulted in a significant inward proton translocation. 6. The possibility that isocitrate might normally be oxidized via loop 0 at a redox potential of -450mV, or even more negative, is discussed, and implies that a P/O quotient of 4 for isocitrate oxidation might be expected.


The distribution of cardioactive agents among molluscan species and tissues.


Norepinephrine and dopamine in the developing chick brain.


A report on the dental health of Zambian school children in the rural and urban areas of the country.


The effect of the  -receptor blockade (propranolol) on the endocrine and exocrine pancreatic function in man after the administration of intestinal hormones (secretion and cholecystokinin-pancreozymin).


Survival and endogenous colony formation in irradiated mice grafted with normal or infectious mononucleosis bone marrow.


Effects of 2-BR-alpha-ergokryptine (CB 154) on sex-linked rejection of pituitary isografts in female C57BL mice.


The treatment of insomnia through the use of electrosleep: an EEG study.


Treatment of psychosis subsequent to phenothiazine-induced agranulocytosis.


Drug effects on regional cerebral blood flow in focal cerebrovascular disease.


Beta blockade in the treatment of tricyclic antidepressant overdosage.


The aromatization of (7 -3H) androstenedione by human placental mitochondria.


Seminar on dorsal column stimulation: summary of proceedings.


Cutaneous polyarteritis nodosa and Crohn's disease.


Effects of iodinated contrast media on erythrocytes. II. Effect of some drugs on alterations induced by meglumine acetrizoate.


Deoxyribonucleic acid base composition of staphylococci.


The direct demonstration of phage microlysis in thin sections of colonies of argentinian Mycobacterium bovis strains.


Antibiotic activity of polysaccharide--protein complexes isolated from different candida species.


Studies on the suitability of sulphite cycloserine agar for the enumeration of clostridium perfringens in food and water.


On the release of prostaglandin E2 from the rabbit heart following infusion of noradrenaline.


Hyperkinetic cardiovascular state in polymyositis.


Metabolism of androst-4-ene-3,17-dione by subcellular fractions of rat adrenal tissue with particular reference to microsomal C19-steroid 5alpha-reductase. The location and some characteristics of rat adrenal C(19)-steroid 5alpha-reductase were investigated by using [7alpha-(3)H]androst-4-ene-3,17-dione and [7alpha-(3)H]testosterone as substrates. The enzymes system was shown to be NADPH-dependent and associated with the microsomal fraction. In addition, some evidence was also obtained for the existence of a separate NADH-dependent system in the soluble fraction. Further investigation of androst-4-ene-3,17-dione metabolism by subcellular fractions indicated the presence of NADH-dependent 3alpha- and 3beta-hydroxy steroid dehydrogenase systems in the microsomal pellet. This pellet also appeared to contain an NADH-dependent 17beta-hydroxy steroid dehydrogenase system, and a similar though separate system was detected in the cytosol. Malate (20mm) effectively inhibited the microsomal C(19)-steroid 5alpha-reductase, which showed similar values for K(m) and V(max.) when either androst-4-ene-3,17-dione or testosterone was used as substrate. Cytochrome c was added to all incubation mixtures used for the determination of these values to inhibit the formation of metabolites other than 5alpha-androstane-3,17-dione and 5alpha-dihydrotestosterone (17beta-hydroxy-5alpha-androstan-3-one) respectively. It was also found that corticosterone did not inhibit the 5alpha-reduction of androst-4-ene-3,17-dione under these conditions, indicating that separate enzymes exist for the 5alpha-reduction of C(19)- and C(21)-steroids in the rat adrenal.


The isocitrate dehydrogenases of Acinetobacter lwoffi. Studies on the regulation of a nicotinamide-adenine dinucleotide phosphate-linked isoenzyme. Of the two NADP-linked isocitrate dehydrogenases in Acinetobacter lwoffi the higher-molecular-weight form, isoenzyme-II, is reversibly stimulated sixfold by low concentrations of glyoxylate or pyruvate. Kinetic results indicate that this stimulation of activity involves both an increase in V(max.) and a decrease in the apparent K(m) values for substrates, most markedly that for NADP(+). Other changes brought about by glyoxylate or pyruvate include a shift in the pH optimum for activity and an increased stability to inactivation by heat or urea. Mixtures of glyoxylate plus oxaloacetate, known to inhibit isocitrate dehydrogenases from other organisms, produce inhibition of both A. lowffi isoenzymes, and do not reflect the stimulatory specificity of glyoxylate for isoenzyme-II. Isoenzyme-II is also stimulated by AMP and ADP, but the activation by glyoxylate or pyruvate is shown to be quite independent of the adenylate activation. Differential desensitization of the enzyme by urea to the two types of activator further supports the view that the enzyme possesses two distinct allosteric regulatory sites. The metabolic significance of the activations is discussed.


Tooth survival following various methods of sub-apical osteotomy.


Current trends in health care. The physician's role.


Eearly pulp changes following reduction of open bite by segmental surgery.


Automated assay of single tablets of methdilazine and methdilazine hydrochloride.


Equilibrium geometries of three beta adrenergic blocking agents.


Clemmensen reduction of Mannich bases: potential ganglionic and nicotinic agents.


The effects of age and sex on the biosynthesis, urinary excretion and tissue concentration of L-ascorbic acid in rats.


Effect of fructose on cellular respiration in perfused rat liver.


Metabolism of fructose and glyceraldehyde in the isolated perfused pig liver.


The interrelationship between fructose and ethanol metabolism in the isolated perfused pig liver.


The effect of fructose on hepatic synthesis of fatty acids.


Ventricular function curve analysis of the myocardial effects of three beta blockers.


An experimental screening for "systemic adjuvants of immunity" applicable in cancer immunotherapy.


Relative activities of acetyl-CoA carboxylase and fatty acid synthetase in chick liver: effects of dietary fat.


Immunosuppression and malignant lymphomas in graft-versus-host reactions.


Immune capacity and response to antigenic tumors.


Studies on glutathione reductase and regeneration of reduced glutathione in normal human adult and cord red cells.


Effects of allethrin and Condylactis toxin on the kinetics of sodium conductance of crayfish axon membranes.


Seasonal variation in heart-rate-core temperature relationship in Rana pipiens: the role of the autonomic nervous system.


Antagonism between bicuculline, strychnine, and picrotoxin and depressant amino-acids in the rat nervous system.


A reappraisal of the actions of noradrenaline and 5-hydroxytryptamine on cerebral cortical neurons.


The quantitative analysis of some constituents of crude sweat. II. Zinc, copper, iron, sialic acid content and oxidative activity.


Caries experience and fluoride uptake in children receiving semiannual prophylaxes with an acidulated phosphate fluoride paste. Questionable conclusions?


Methods of solid tumour evaluation in immunological experiment. I. Objective error in the determination of the growth curve.


ACS establishes standards for surgeon's assistants.


Drug reactions and sublingual testing with certified food colors.


Dietary influence on antinuclear antibodies and cell-mediated immunity in NZB mice.


D-alanine oxidase form Escherichia coli: localization and induction by L-alanine. Dialyzed membranes of Escherichia coli prepared by an ethylenediaminetetraacetic acid-lysozyme method catalyze the oxidation of both l-alanine and d-alanine. The specific activities for the oxidations of both d-alanine and l-alanine are increased fivefold when the cells are grown in the presence of either l-alanine or dl-alanine, but are increased only slightly when grown in the presence of d-alanine. In the dl-alanine-induced system, the specific activities for the oxidations of some other d-amino acids are also raised. dl-alanine also induces two other alanine catabolizing enzymes, alanine dehydrogenase and alanine-glutamate aminotransferase which are found in the "soluble" fraction of lysozyme-treated cells. The oxidations of both l-alanine and d-alanine were associated with the membranes of induced cells. After the membranes were disintegrated by sonic treatment, both l-alanine and d-alanine oxidation catalysts sedimented in a sucrose density gradient together with d-lactate and l-lactate dehydrogenases, apparently as a single multienzyme complex.


D-alanine oxidase from Escherichia coli: participation in the oxidation of L-alanine. Cell wall-membrane preparations of Escherichia coli, prepared by the ethylenediaminetetraacetic acid-lysozyme method, contain enzymes which catalyze the oxidation of d-alanine and, to a lesser extent, l-alanine into pyruvate and ammonia without the formation of hydrogen peroxide. The kinetic parameters were (i) pH optima of 8.3 to 8.4 for l- and d-alanine and (ii) a K(m) value of 6.6 +/- 0.2 mM for d-alanine. Several coenzymes were without effect when added to the reaction mixture. The participation of d-alanine oxidase in the oxidation of l-alanine was demonstrated. The evidence is based on (i) results of cellular fractionation; (ii) labeling experiments; (iii) inhibition studies with aminooxyacetate and cycloserine; (iv) denaturation experiments; and (v) demonstration of the presence of an active racemase.


Pathway of n-alkane oxidation in Cladosporium resinae. Pathways of initial oxidation of n-alkanes were examined in two strains of Cladosporium resinae. Cells grow on dodecane and hexadecane and their primary alcohol and monoic acid derivatives. The homologous aldehydes do not support growth but are oxidized by intact cells and by cell-free preparations. Hexane and its derivatives support little or no growth, but cell extracts oxidize hexane, hexanol, and hexanal. Alkane oxidation by extracts is stimulated by reduced nicotinamide adenine dinucleotide (phosphate). Alcohol and aldehyde oxidation are stimulated by nicotinamide adenine dinucleotide (phosphate), and reduced coenzymes accumulate in the presence of cyanide or azide. Extracts supplied with (14)C-hexadecane convert it to the alcohol, aldehyde, and acid. Therefore, the major pathway for initial oxidation of n-alkanes is via the primary alcohol, aldehyde, and monoic acid, and the system can act on short-, intermediate-, and long-chain substrates. Thus, filamentous fungi appear to oxidize n-alkanes by pathways similar to those used by bacteria and yeasts.


Sectral in the management of angina pectoris.


Antibodies to an extract of Streptococcus mutans, containing glucosyltransferase activity, related to dental caries in man.


The part played by the trans-septal fibre system in experimental approximal drift of the cheek teeth of monkeys (Macaca irus).


Effects of drugs on the nose and middle ear. Lessons from an animal model.


Epidemiology of traumatic dental injuries to primary and permanent teeth in a Danish population sample.


Application of a new chemically bonded, superficially porous anion-exchange packing to nucleotide analysis.


Health manpower development and rural services.


The management of hypertension.

The management of hypertension.


Production of antibodies to bovine adrenal tyrosine hydroxylase: cross-reactivity studies with other pterin-dependent hydroxylases.


Enzymatic reduction of "biogenic" aldehydes in brain.


Reduced diphosphopyridine nucleotide synergism of the reduced triphosphopyridine nucleotide-dependent mixed-function oxidase system of hepatic microsomes. I. Effects of activation and inhibition of the fatty acyl coenzyme A desaturation system.


Reduced diphosphopyridine nucleotide synergism of the reduced triphosphopyridine nucleotide-dependent mixed-function oxidase system of hepatic microsomes. II. Role of the type I drug-binding site of cytochrome P-450.


Non-esterified fatty acids, serum insulin and blood glucose after a beta-receptor blocking agent in normal, hypophysectomized, diabetic and hypophysectomized-diabetic dogs.


Choline acetylase and glutamic acid decarboxylase in Huntington's chorea. A preliminary study.


Testosterone metabolism in slices and subcellular fractions of human liver.


Metabolism of 5alpha-dihydrotesterone in the rat prostate.


5Alpha-steroid reductase in human fetal brain.


Enzymes of androgen metabolism in human leucocytes.


The effect of gonadotropins on the "androgen status" in boys with undescended testes.


The dietary control of the microsomal stearyl CoA desaturation enzyme system in rat liver.


Phosphorus-31 nuclear magnetic resonance studies of the phosphonate and phosphate composition of the sea anemone, Bunadosoma, sp.


Kinetics of binding of reduced nicotinamide adenine dinucleotide phosphate to the rat liver fatty acid synthetase complex.


Pharmacological properties of ((3alpha-tropanyl)oxy)-5H-benzo(4,5)cycloheptal(1,2-b)-pyridine hydrogen maleate (BS 7723).


On model building in structure-activity relationships. A reexamination of adrenergic blocking activity of beta-halo-beta-arylalkylamines.


Comparison of the responses of density gradient separated lymphocytes to phytohemagglutinin and histocompatibility antigens.


Detection of nonspecific cytotoxicity in graft-versus-host reaction as a function of target cell type.


Preparation and cardiovascular actions of a group of tetrahydroisoquinoline derivatives.


The mosquito: still man's worst enemy.

The mosquito: still man's worst enemy.


Occurrence of Mycobacterium leprae in arthropods.


Catecholamine and dibutyryl cyclic AMP effects on myosin adenosine triphosphatase in cultured rat heart cells. Catecholamines and dibutyryl adenosine 3', 5'-monophosphate (dibutyryl cyclic AMP) increase the activity of myosin adenosine triphosphatase in cultured rat heart cells. Dichloroisoproterenol, an inhibitor of the beta receptor of the catecholamines, inhibits the action of the catecholamines but not of cyclic AMP.


Some aspects of aviation physiology.

Some aspects of aviation physiology.


A comparative study of the absorption of xanthinol nicotinate from slow-release and plain tablets in man.


Nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase of Neurospora. I. Isolation, subunits, amino acid composition, sulfhydryl groups, and identification of a lysine residue reactive with pyridoxal phosphate and N-ethylmaleimide.


Role of pyruvate carboxylase, phosphoenolpyruvate carboxykinase, and malic enzyme during growth and sporulation of Bacillus subtilis.


Regulation of the synthesis of enzymes responsible for glutamate formation in Klebsiella aerogenes.


Metabolism of 3-beta-hydroxy-5-ene steroids by testes of rats acclimatized to a hot environment.


The role of dietary protein in generating daily rhythms in rat liver tryptophan pyrrolase and tyrosine transaminase.


Uses and misuses of anti-inflammatory drugs in racehorses. 1.


Urinary excretion of phenothiazine tranquillisers by the horse.


Multidimensional analysis of subjective symptomatology during prolonged strenuous exercise.


Immune surveillance of lymphoid tissue. A biological role for the mixed lymphocyte reaction.


Replicated correlations of MMPI two-point code types: the Missouri actuarial system.


Endorsement of Ellis' irrational beliefs as a function of psychopathology.


The Mini-Mult and its use with adolescents.


Primary affective disorder: MMPI differences between unipolar and bipolar depressed subjects.


Stages of schizophrenic decompensation and reintegration.


Psychoactive drug-induced changes of interhemispheric EEG amplitude relationships.


Effect of cyclophosphamide on a local graft-versus-host reaction in the rat: influence of sex, disease and different dosage regimens.


Disk test for the differentiation of pneumococci from other alpha-hemolytic streptococci. One hundred twenty-five strains of Diplococcus pneumoniae and one hundred twenty-five strains of other alpha-hemolytic streptococci were planted on horse red cell-Brucella agar, and a disk of methicillin was placed on each inoculated plate. All strains were incubated anaerobically at 37 C for 24 h and then exposed to air at 6 C for 48 h. All strains of pneumococci produced a ring of beta-hemolysis surrounding the zone of inhibition produced with the methicillin disk, whereas no strain of alpha-hemolytic streptococci produced beta-hemolysis. It is suggested that this new criterion is useful for the differentiation of pneumococci from other alpha-hemolytic streptococci in those cases where other characteristics were insufficient for an unequivocal identification.


The effect of experimentally induced redox changes on human rheumatoid and non-rheumatoid synovial tissue in vitro.


Drug therapy in the elderly.

Drug therapy in the elderly.


Personality correlates of venereal disease experience.


Treatment of cardiogenic shock. I.

Treatment of cardiogenic shock. I.


A device for the mounting and manipulation of small protein crystals.


Testicular enzymes and spermatogenesis.

Testicular enzymes and spermatogenesis.


Spectrophotometric studies of the mechanism of photosynthesis.


Metabolites of Serc and interaction of Serc with antihistamines.


"How should a treatment of Méniére's syndrome be evaluated?" Panel discussion.


Early arrhythmias following experimental coronary occlusion in conscious dogs and their modification by beta-adrenoceptor blocking drugs.


An approach to the evaluation of oral antihistaminic preparations using inhibitory action of histamine skin response in dogs.


Non-specific inhibition of the precipitin reaction between teichoic acids and antisera.


Quantitative studies of the specificity of anti-pneumococcal polysaccharide antibodies, types 3 and 8. V. Cross-reacting antibodies in rabbit antisera.


Non-hemorrhagic arthus reaction and its independence from circulating antibodies and complement.


Pharmacological properties of a proposed mediator of dealyed hypersensitivity reactions.


Nonantipsychotic phenothiazines and tardive dyskinesia.


Why not train your own assistant?

Why not train your own assistant?


Ambulatory care and rehabilitation in COPD.


Stimulation of sympathetic nerve fibres to the nose in cats.


A national nutrition policy and program.


Personality characteristics of servicemen returned from Viet Nam identified as heroin abusers.


The diagnostic decision-making process: factors influencing diagnosis and changes in diagnosis.


Patterns of professional activities of psychiatrists: a follow-up of 100 psychiatric residents.


Epidemiological studies of women prisoners. I. Medical and psychiatric variables related to violent behavior.


Perinatal development of hepatic microsomal mixed function oxidase activity in swine.


Oxidative demethylation of 14C-griseofulvin by liver microsomes of rats and mice.


Formation of catecholamines from phenylalanine in brain--effects of chlorpromazine and catron.


Central effects of alpha-adrenergic blocking agents on thermoregulation against venous blood-stream cooling in unanesthetized rabbits.


Separation of soluble denitrifying enzymes and cytochromes from Pseudomonas perfectomarinus.


Latency and sedimentability of acid hydrolases in sterile septa homogenates of Cerianthus lloydi G.


Trimethylamine oxidase of nurse shark liver and its relation to mammalian mixed function amine oxidase.


Significance of cholesterol esters as liquid crystal in human atherosclerosis.


Study on the transport of fatty acids in the arterial wall.


The suppressive activities of alprenolol against deslanoside-induced cardiotoxicities in guinea pigs.


An indirect action of papaverine in smooth muscle in vivo.


Presence of histamine H2-receptors in the guinea-pig pulmonary vascular bed.


Determination of free and acetylated 5-aminosalicylic acid in serum and urine after administration of salicylazosulphapyridine.