Beta-adrenoceptor blocking agents and responses to adrenaline and 5-hydroxytryptamine in rat isolated stomach and uterus. 1 Four beta-adrenoceptor blocking agents, (+/-)- and (+)-propranolol, practolol and oxprenolol, were found to antagonize, apparently competitively, the responses of both the rat isolated stomach and uterus to 5-hydroxytryptamine (5-HT).2 The pA(2) values for each of these agents as antagonists of the contractile action of 5-HT on the rat stomach were found to be: (+/-)-propranolol, 6.08; (+)-propranolol, 4.94; practolol, 3.43; and oxprenolol, 5.99. These values were very similar to the corresponding figures for antagonism of 5-HT-induced contractions of the uterus.3 pA(2) values for antagonism of adrenaline-induced relaxations by the four blocking agents on the rat stomach and uterus did not differ from the values for 5-HT blockade.4 To antagonize contractile responses to acetylcholine of the rat stomach it was necessary to give 100 times more (+/-)-propranolol than was needed to antagonize responses to 5-HT.


Evidence for involvement of 5-hydroxytryptamine in the actions of amphetamine. 1 Pargyline treatment, 1 h before (+)-amphetamine (1 mg/kg), reduced amphetamine-stimulated motor activity. This inhibition was reversed in animals pretreated with p-chlorophenylalanine (PCPA).2 Following treatment with PCPA or 5,6-dihydroxytryptamine (5,6-DHT), amphetamine-induced locomotor activity was significantly potentiated. The increased response to amphetamine in PCPA-treated rats was reversed in animals pretreated with 5-hydroxytryptophan.3 The inhibition of amphetamine-stimulated locomotor activity by treatment with 6-hydroxydopamine was not reversed by PCPA treatment.4 Stereotypies produced by amphetamine were not found to be altered by depletion of 5-hydroxytryptamine.5 Induction of adrenal tyrosine hydroxylase activity produced by chronic amphetamine administration was significantly potentiated by PCPA, emphasizing the involvement of a 5-hydroxytryptamine inhibitory system in more than one action of amphetamine.


Comparison of the effects of propranolol and ICI 66082 in blocking the renin releasing effect of renal nerve stimulation in the cat. The comparative effectiveness of propranolol and ICI 66082, in inhibiting the release of renin from the kidney resulting from renal nerve stimulation, has been studied in the cat. Over the dose range 0.17-0.68 x 10(-5) mol/kg propranolol there was a dose-response relationship with the increase of plasma renin activity (PRA) achieved after 10 min stimulation. Over the dose range 0.75-1.69 x 10(-5) mol/kg ICI 66082 also inhibited the release of renin caused by nerve stimulation, but was about five times less effective than propranolol. It would appear from these data that the beta-adrenoceptors within the kidney mediating renin release are distinctly different from those of the heart.


Influences of some freshwater plants on the development and survival of mosquito larvae in British Columbia.


Oxidative phosphorylation by chondrocyte mitochondria.


Soluble factors and the immune response: in vitro studies of the immunosuppression induced by the graft-versus-host reaction.


Inhibition of erythroid cell growth by allogeneic murine lymphocytes. Evidence for a synergism between lymph node cells and thymocytes.


Use of bromazepam in obsessional, phobic and related states.


Disorders of the autonomic nervous system.  Chapter 4.  Disorders of cardiac control.


Decoding of regulatory signals at the microbial surface.


Simultaneous determination of sulthiame and diphenylhydantoin serum levels by gas-liquid chromatography.


Proceedings: An abnormality of secondary energy resources in epileptogenic human brain.


Cost containment: The buyer's nightmare.


Coordinate regulation of enzyme inducibility and developmental competence in Aspergillus nidulans.


Coexistence of raynaud's phenomenon and chronic active hepatitis with hepatitis B antigen.


Provocative approaches to materiel management.


Opinion polls provide valuable feedback: ask the patient.


The art and science of materiel management.


The trick of materiel management.

The trick of materiel management.


Editorial: Humanizing patient care.

Editorial: Humanizing patient care.


A dissenting opinion: Miller vs. Unity Hospital.


Location of NADH-oxidase activity in fractions of Tritrichomanas foetus homogenate.


Effects of anti-T cell (theta) and anti-B cell (beta) serum on the immune response in mice.


Activity of the reticuloendothelial system and the antibody response. III. The fate of type III pneumococcal polysaccharide and the antibody response.


Immunological disease induced by injecting F1 lymphoid cells into certain parental strains.


The action of some sympathomimetic amines on the neuromuscular junction.


Regulation of gene expression in mammalian cells.


Problems of cryptorchidism.

Problems of cryptorchidism.


Evaluation of gonadal function in childhood and adolescence.


Some properties of glutamine synthetase from baker's yeast.


Succinate- and NADH oxidase systems of Escherichia coli membrane vesicles. Mechanism of selective inhibition of the systems by zinc ions.


Separation and analysis of psychopharmacologic drugs by high efficiency liquid chromatography.


An evaluation of low voltage counterimmunoelectrophoresis for the detection of hepatitis-B antigen (HB Ag). A newly available low voltage counterimmunoelectrophoresis (CIEP) system for the detection of HB Ag (Hapindex, Ortho Diagnostics) was compared with a conventional CIEP method used at this laboratory. A total of 1216 sera were tested. The Hapindex system was found to be at least as sensitive as the conventional CIEP. No false positives were found in this series. The elimination of any preparative work makes the Hapindex system particularly suitable for laboratories not testing large numbers of sera for HB Ag. It also eliminates many of the contamination hazards inherent in the conventional method.


The cold pressor response in liver cirrhosis.


Effect of beta-alanine on glucose and fructose catabolism in Drosophila melanogaster with notes on beta-aminoisobutyric and gamma-aminobutyric acids.


Estimation of effect of drugs on the nasal circulation.


Inappropriate directions in the evolution of the physicians assistants.


Simple chemical synthesis of a specific effector for the affinity chromatography of nicotinamide adenine dinucleotide phosphate-dependent dehydrogenases.


Steroidogenesis in vitro by the ovarian tissue of the domestic fowl (Gallus domesticus).


Psychophysiology of pain: diagnostic and therapeutic implications.


Physician extenders: divergent views and implications for the future.


The computer in family practice.

The computer in family practice.


Toward consumer-oriented health care.

Toward consumer-oriented health care.


Changes in conduction velocity within a nerve net.


Cerebral energy reserves and glycolysis in neural tissue of 6-aminonicotinamide-treated mice.


Isolation of hydrophobic proteins binding neurotransmitter amino acids: Gamma-aminobutyric acid receptor of the shrimp muscle.


Subunit structure and kinetic properties of 4-aminobutyrate-2-ketoglutarate transaminase purified from mouse brain.


L-glutamic acid decarboxylase in non-neural tissues of the mouse.


Metabolic compartmentation of glutamate associated with the formation of gamma-aminobutyrate.


Metabolic compartmentation in the brain: metabolism of a tricarboxylic acid cycle intermediate, (1,4-14C)succinate, after intracerebral administration.


Occupational medicine's new resource.

Occupational medicine's new resource.


Membrane permeability change during inhibitory transmitter action in crayfish stretch receptor cell.


Letters to the editor: Psychogenic backache.


Laser effect on vital oral tissues: a preliminary investigation.


Half-life of diphenylpyraline in man.

Half-life of diphenylpyraline in man.


Spectrofluorodensitometric determination of flurazepam and its major metabolites in blood.


GLC determination of propranolol, other beta-blocking drugs, and metabolites in biological fluids and tissues.


Testicular carnitine acetyltransferase activity and serum testosterone levels in developing and cryptorchid rats.


Induction of lethal graft versus host disease in rats by spleen grafting.


General principles of assays for adenylate cyclase and guanylate cyclase activity.


Preparation of vertebrate photoreceptor membranes for study of adenylate cyclase, guanylate cyclase, and cyclic nucleotide phosphodiesterase.


Preparation and characterization of guanylate cyclase from bovine lung.


Guanylate cyclase from sperm of the sea urchin, Strongylocentrotus purpuratus.


Separation and purification of cyclic nucleotides by alumina column chromatography.


Guanylate cyclase in human platelets.

Guanylate cyclase in human platelets.


Separation and purification of cyclic nucleotides by ion-exchange resin column chromatography.


Adverse Drug Reactions Advisory Committee report for 1973.


MMPI correlates of localized cerebral lesions.


Phenelzine in phobic states.

Phenelzine in phobic states.


The oral health of children of dentists.


Fissure sealing with Nuva-Seal in a public health program for Danish schoolchildren after 12 months' observation.


Histochemistry of the epithelium in excisional palatal wounds of guinea pigs.


Enzyme activities of the perfused ex-vivo porcine liver: importance of a physiological perfusate.


Duplication of penis.

Duplication of penis.


Testicular nondescent versus testicular agenesis: importance of diagnosis and correct surgical management.


Effects of alpha and beta receptor stimulating and blocking agents on experimental disseminated intravascular coagulation. In this experimental study on rabbits, we compare the haemostatic and histological changes observed in animals with disseminated intravascular coagulation (DIC) induced by thrombin perfusion (100 u/kg/hr during 3 hours) with or without preventive administration of alpha and beta receptor stimulating or blocking agents. Both alpha and beta receptor stimulating agents enhance the disorders induced by thrombin alone, while alpha or beta blocking agents reduce them. This favourable action is still more pronounced with the combination of these two last types of drugs. The modifications observed appear to be related to changes in platelet function.


Reference materials and methods in clinical chemistry. Report of a Discussion Group Meeting held on the occasion of the 1st European Congress of Clinical Chemistry. Munich, 23rd April 1974.


Problems of insecticide resistance.

Problems of insecticide resistance.


Conditioning in the prediction of drug withdrawal effects in chronic schizophrenic patients.


Insulin and fat metabolism.

Insulin and fat metabolism.


Bacterial flora of the normal conjunctiva. I. Topographical distribution.


Activity of gamma-glutamyl-transpeptidase (GGTP) and leucyl-arylamidase (LAP) in the renal tubule during stop flow in dogs, in various ischemic states.


Urinary screening for drugs in a hospital for the mentally subnormal.


An electron microscopic study of influenza virus reproductive cycle in chick embryo cells with selective inhibition of host cell macromolecular synthesis.


Thymidine kinase induction in mouse embryo cells by polyoma virus. II. Effect of 5-fluorouracil.


Strong inhibition of transfection by critical low concentration of diethylaminoethyl-dextran.


Ribonuclease activity tightly bound to ribosome-like structures isolated from avian myeloblastosis virus.


Professor Frantisek Patocka--Septuagenarian.


Adaptation of herpes simplex virus type 1 to supraoptimal temperature. II. Immunofluorescent and cytological study of virus-infected cells, adapted and non-adapted to 40 degrees C.


Rickettsial plaques in mosquito cell monolayers.


Aetiology of phlebotomus fever in Ashkhabad studied in retrospect.


Pathogenicity of mengo virus to mice. I. Virological studies.


Behaviour of two herpes simplex virus strains of genital and non-genital origin carried through serial passages in WI-38 cells.


Regulation and suppression of graft-versus-host reaction.


Liver microsomal electron transport systems. III. The involvement of cytochrome b5 in the NADPH-supported cytochrome P-450-dependent hydroxylation of chlorobenzene.


Microbial oxidation of amines. Partial purification of a trimethylamine mono-oxygenase from Pseudomonas aminovorans and its role in growth on trimethylamine. 1. A mono-oxygenase, which oxidizes trimethylamine and other tertiary amines bearing methyl or ethyl groups, was partially purified sixfold from Pseudomonas aminovorans grown on trimethylamine as sole carbon source. 2. The preferred electron donor was NADPH. The enzyme had a pH optimum of 8.0-9.4 for trimethylamine oxidation, and 8.8-9.2 for dimethylamine oxidation. 3. The oxidation product of trimethylamine was shown to be trimethylamine N-oxide. Other tertiary amines were probably also converted into N-oxides. 4. The enzyme also oxidized secondary amines. 5. The oxidation of trimethylamine was only slightly inhibited by CO and not at all by KCN or proadifen hydrochloride (SKF 525-A), but was inhibited by trimethylsulphonium chloride, tetramethylammonium chloride, 2,4-dichloro-6-phenylphenoxyethylamine (Lilly 53325) and its NN-diethyl derivative (Lilly 18947). 6. The oxidation of dimethylamine showed a similar response to inhibitors and a parallel loss in activity on heating at 35 degrees C. 7. The activities of the trimethylamine mono-oxygenase, trimethylamine N-oxide demethylase and the secondary-amine mono-oxygenase increased severalfold during adaptation of succinate-grown bacteria to growth on trimethylamine, and the trimethylamine mono-oxygenase was the first enzyme to show an increase in activity. It is concluded that all three enzymes are involved in growth on trimethylamine by this organism.


Metabolic differences between the arteries of atheroscierotic susceptible and resistant pigeons.


Microsomal (muS)1 lipid peroxidation, drug oxidations and chemiluminescence (CL): mechanisms.


Regulation of ornithine decarboxylase activity by putrescine and spermidine in rat liver.


The isolation and subfractionation of plasma membrane from the cellular slime mould Dictyostelium discoideum. A procedure for the isolation and separation of three different subfractions of plasma membrane from the cellular slime mould Dictyostelium discoideum is described. The cells were disrupted by freeze-thawing in liquid N(2) and plasma membranes were purified by equilibrium centrifugation in a sucrose gradient. The cell surface was labelled with radioactive iodide by using the lactoperoxidase iodination method. Alkaline phosphatase was identified as a plasma-membrane marker by its co-distribution with [(125)I]iodide. 5'-Nucleotidase, which has been widely described as a plasma-membrane marker enzyme in mammalian tissues, was not localized to any marked extent in D. discoideum plasma membrane. The isolated plasma membranes showed a 24-fold enrichment of alkaline phosphatase specific activity relative to the homogenate and a yield of 50% of the total plasma membranes. Determination of succinate dehydrogenase and NADPH-cytochrome c reductase activities indicated that the preparation contained 2% of the total mitochondria and 3% of the endoplasmic reticulum. When the plasma-membrane preparation was further disrupted in a tight-fitting homogenizer, three plasma-membrane subfractions of different densities were obtained by isopycnic centrifugation. The enrichment of alkaline phosphatase was greatest in the subfraction with the lowest density. This fraction was enriched 36-fold relative to the homogenate and contained 19% of the total alkaline phosphatase activity but only 0.08% of the succinate dehydrogenase activity and 0.34% of the NADPH-cytochrome c reductase activity. Electron microscopy of this fraction showed it to consist of smooth membrane vesicles with no recognizable contaminants.


Microbial metabolism of the pyridine ring. The hydroxylation of 4-hydroxypyridine to pyridine-3,4-diol (3,4-dihydroxypyridine) by 4-hydroxypyridine-3-hydroxylase. 1. The first metabolic step in the biodegradation of 4-hydroxypyridine by an Agrobacterium sp. was hydroxylation to form pyridine-3,4-diol. 2. Extracts required 1mol of O(2) and 1mol of NADH or NADPH for the conversion of 4-hydroxypyridine into pyridine-3,4-diol, suggesting that the enzyme responsible, 4-hydroxypyridine-3-hydroxylase, was a mixed function mono-oxygenase. 3. After treatment with acidic (NH(4))(2)SO(4) the enzyme required FAD for activity; FMN and riboflavin would not substitute for FAD. 4. The rate of anaerobic reduction of FAD by NAD(P)H was increased more than tenfold in the presence of 4-hydroxypyridine, suggesting that the mechanism of hydroxylation was similar to that of other aromatic hydroxylases which are of the mono-oxygenase type. 5. The partially purified enzyme was extremely specific for its heterocyclic substrate but would utilize either NADH or NADPH. 6. 4-Hydroxypyridine-3-hydroxylase was strongly inhibited by high substrate concentration (above 0.5mm) especially below pH7.5. 8. The inflexion at pH8.4 in a pK(m) versus pH plot, together with strong inhibition by p-chloromercuribenzoate, suggested a role for thiol groups in substrate binding.


Lipogenesis in rat and guinea-pig isolated epididymal fat-cells. Fat-cells were prepared from rat and guinea-pig epididymal adipose tissue and compared on the basis of the intracellular distributions and activities of enzymes and with respect to their utilization of various U-(14)C-labelled substrates for lipogenesis. 1. Compared with the rat, guinea-pig extramitochondrial enzyme activities differed in that aconitate hydratase, alanine aminotransferase, ATP-citrate lyase, lactate dehydrogenase, NAD-malate dehydrogenase, NADP-malate dehydrogenase and phosphoenolpyruvate carboxykinase activities were appreciably lower, whereas aspartate aminotransferase, glucose 6-phosphate dehydrogenase, NADP-isocitrate dehydrogenase and 6-phosphogluconate dehydrogenase activities were appreciably higher. Mitochondrial activities of citrate synthase, NADP-isocitrate dehydrogenase and pyruvate carboxylase were appreciably lower, whereas mitochondrial activities of aspartate aminotransferase, glutamate dehydrogenase, NAD-malate dehydrogenase and phosphoenolpyruvate carboxykinase were higher in the guinea pig compared with the rat. 2. In general guinea-pig fat-cells incorporated acetate and lactate into fatty acids more readily than rat fat-cells, whereas rat fat-cells incorporated glucose and pyruvate more readily than guinea-pig fat-cells. 3. Acetate stimulated the incorporation of glucose into fatty acids in rat fat-cells, but had no appreciable effect upon this process in guinea-pig fat-cells. Acetate greatly decreased the incorporation of lactate into fatty acids in cells from both species. 4. Lactate/pyruvate ratios produced by incubation of guinea-pig cells with glucose+insulin were very low compared with those found with rat cells under the same conditions. 5. With glucose (+insulin) or with glucose+acetate (+insulin) as substrates guinea-pig cells produced enough NADPH by the hexose monophosphate pathway to satisfy the NADPH requirements of lipogenesis. In rat fat-cells under the same conditions, hexose monophosphate-pathway NADPH provision was not sufficient to meet the requirements of lipogenesis. 6. These results are discussed, particularly in relationship to the disposition of cytosolic reducing equivalents in the cells.


Comparison of the amino acid sequences of the variable regions of light chains derived from two homogeneous rabbit anti-pneumococcal antibodies. The amino acid sequence of the N-terminal 139 residues of the L (light) chain derived from a homogeneous rabbit antibody to type III pneumococci was determined. This L chain, designated BS-5, exhibits a greater degree of homology with the basic sequence of human kappa chains of subgroup I (72%) than with subgroups II and III. L-chain BS-5 differs from another L chain (BS-1), also derived from an antibody to type III pneumococci (Jaton, 1974), by eight amino acid residues, even though the chains are identical within the N-terminal 30 residues. Six of these eight substitutions are located within the three hypervariable sections of the variable half: Asn/Ser in position 31, Glu/Ala in position 55, Asx/Thr, Thr/Gly, Thr/Gly and Val/Tyr in positions 92, 94, 96 and 97 respectively. The two anti-pneumococcal L chains BS-1 and BS-5 are much more similar to each other than to an anti-azobenzoate L chain (Appella et al., 1973), from which they differ by 30 and 29 residues respectively. Of these interchanges 13-15 are confined to the three hypervariable sections, and 11 occur within the N-terminal 27 positions. The three chains have an identical sequence from residue 98 to residue 139, except for a possible inversion of two residues in positions 130-131 of the anti-azobenzoate chain.


Genetic variation in histidine ammonia-lyase activity in the mouse.


Clinical testing of dicalcium phosphate supplemented sweets. (2). Caries prevalence in subjects aged 3 to 14 years following a maximum of three years' exposure to the sweets.


A preliminary double-blind study with SB 5833 (camazepam), a new benzodiazepine derivative.


Clinical testing of dicalcium phosphate supplemented sweets. (1). 3-year caries incidence in subjects aged 11 to 15 years.


Neuro-pharmacological studies on SB 5833, a new psychotherapeutic agent of the benzodiazepine class.


The effect of certain drugs on amphetamine-induced stereotyped behavior and brain amphetamine levels in the rat.


Patterns of admission in schizophrenia.

Patterns of admission in schizophrenia.


The effect of a polychlorinated biphenyl (Aroclor 1254) on liver microsomal enzymes in the male rat.


Genetic control of the immune response of mice to type III pneumococcal polysaccharide.


Allogeneic interactions in the immune response and tolerance. I. The allogeneic effect in unprimed rats and mice.


Cross-reactivity patterns of mouse lymphocytes sensitized against the major histocompatibility complex using a graft-versus-host assay.


Allotype levels in normal and allotype suppressed mice after allogeneic stimulation.


In vivo effects of antithymocyte serum on the homing patterns and graft-versus-host reactivity of murine splenic lymphocytes.


Immune restoration of mice immunosuppressed by a graft-vs.-host reaction.


Local graft-versus-host reaction suppressed by immuno-blocking antibody secreted by antibody-forming cells transferred with the graft.


Methods for studying lipid-protein interactions.


A critical review of the field application of a mathematical model of malaria eradication. A malaria control field research trial in northern Nigeria was planned with the aid of a computer simulation based on Macdonald's mathematical model of malaria epidemiology. Antimalaria attack was based on a combination of mass drug administration (chloroquine and pyrimethamine) and DDT house spraying. The observed results were at great variance with the predictions of the model. The causes of these discrepancies included inadequate estimation of the model's basic variables, and overestimation, in planning the simulation, of the effects of the attack measures and of the degree of perfection attainable by their application. The discrepancies were to a great extent also due to deficiencies in the model. An analysis is made of those considered to be the most important. It is concluded that research efforts should be encouraged to increase our knowledge of the basic epidemiological factors, their variation and correlations, and to formulate more realistic and useful theoretical models.


Activities of some glycolytic enzymes in the major salivary glands of some laboratory animals.


Insulation of furs in water.

Insulation of furs in water.


Bile pigments in the blood serum of fish from the family Cottidae.


Effect of hypothalamic extracts on prolactin secretion in the goldfish, Carassius auratus L.


The effect of repeated stimulation on the escape reflex and the acetylcholinesterase activity in different parts of the central nervous system in the crayfish (Pacifastacus leniusculus and Astacus astacus).


Organic material and calories in the egg of the brown pelican, Pelecanus occidentalis.


Serological differentiation of two species of Taterillus (Rodentia, Gerbillidae) from Senegal: T. gracilis (Thomas, 1892) and T. pygargus (Cuvier, 1832).


Clinical trial of CDP-choline as a medicament for acute pancreatitis and its relation to pancreas and liver damage in acute pancreatitis.


Cellular DNA content of the Mollusca.

Cellular DNA content of the Mollusca.


Blood glucose in crayfish--I. Variations associated with molting.


Depolarization-induced change in the enzymatic radio-iodination of a protein of the internal surface of the squid giant, axon membrane.


Gas exchange and metabolism in the Sirenidae (Amphibia: Caudata)--I. Oxygen consumption of submerged sirenids as a function of body size and respiratory surface area.


A study of the adaptation of marine organisms to changing salinities with special reference to the shore crab Carcinus maenas (L.).


A comparison of the activities of the beta-adrenoceptor agonists MJ9184-1 AND (--)-ISOPRENALINE IN GUINEA-PIG AND CAT PREPARATIONS.


Calcium transport by subcellular fractions of rat, dog and monkey brains.


Digestive enzymes in the salivary gland and midgut of the bug Stenotus binotatus.


Carbon dioxide tension and pH of the blood of the lizard Sauromalus obesus at different temperatures.


Myoglobin concentration in the chicken, especially in the gizzard (a biochemical, light and electron microscopic study).


Temporal changes in amino acid catabolism during arousal from hibernation in the golden-mantled ground squirrel.


Lack of influence of small ligands on the alkaline dissociation of Archachatina marginata haemocyanin and absence of co-operativity in the oxygenation.


The effect of some amino acids on carbohydrate uptake by Trypanosoma lewisi.


Chemoreception and the role of proteins: a comparative study.


Organ culture of Amphiuma means liver: control of glycogen content.


The distribution of the digestive enzymes in the gut of the polychaete Neanthes virens (Sars).


Standard metabolism and body temperature of perameloid marsupials from different environments.


Thermoregulation in perameloid marsupials from different environments.


Water metabolism in perameloid marsupials from different environments.


Seasonal and age variation in the metabolism of the common frog, Rana temporaria L. in northern Finland.


Evolution in ground squirrels--I. Transferrins in holarctic populations of Spermophilus.


Effects of mammalian and host gonadotropins on the ovaries and oviducts of female Texas horned lizards, Phrynosoma cornutum.


Hydroxyproline content of the tadpole tail in response to hypothyroidism.


Excitation-secretion coupling in the release of catecholamines from the in vitro frog adrenal: effects of K+, Ca2+, hypertonicity, Na+ and Ni2+.


Seasonal and sex-bound variation in the carbohydrate metabolism of the liver of the whitefish.


Exogenous 14C-urea distribution in Chimaeras.


Exogenous 14C-urea distribution in rajiformes and teleosts.


Comparative water relations of five species of spiders from different habitats.


Distinct components of neutral amino acid transport in chick small intestine.


The crustacean cuticle--I. Studies on the composition of the cuticle.


Osmoregulation in the adult water boatman, Corisella edulis champion.


Differential in rectal and chest muscle temperature during arousal in Eptesicus fuscus and Myotis sodalis (Chiroptera: Vespertilionidae).


The effect of crustacean eye stalk extract on the endogenous respiration of selected mammalian tissues.


The effect of salinity and sex on the respiration rates of excised gills of the blue crab, Callinectes sapidus.


Studies on the mechanism of uptake of D-glucose by Tetrahymena pyriformis GL.


Adaptations in the water economy of some anuran amphibia.


Single unit discrimination of fish odours released by char (Salmo alpinus L.) populations.


Oxygen consumption in relation to environmental oxygen concentrations in the ampullariid snail Marisa cornuarietis (L.).


Surface activity of various fractions of rabbit lung lavage fluid.


Water content of the salt glands and other avian tissues.


Effect of glucose and other carbohydrates on the toad's glomerular pressure.


The eco-physiological significance of the circulatory mechanics of Lymnaea stagnalis L.


The development of thermoregulation and thyroid function in the marsupial Macropus eugenii (Desmarest).


Erythrocytic ouabain-sensitive and ouabain-insensitive adenosine triphosphatase in various mammalian species;.


Contractility of the in situ hibernating marmot ventricle.


Circadian rhythm of plasma prolactin, growth hormone, glucose and free fatty acid in juvenile kokanee salmon, Oncorhynchus nerka.


Water metabolism of two marsupials--the brush-tailed possum, Trichosurus vulpecula and the rock-wallaby, Petrogale inornata in the wild.


The effect of essential fatty acids deficiency on the nitrogen metabolism of rats.


Biochemical studies on some aspects of protein metabolism in vitamin-D deficient rats.


Comparative biochemical studies of osmo-regulation in Sipunclul--I; Steady-state characteristics of two sipunculids in full-strength sea water.


Anoxia tolerance in two species of toads, Bufo valliceps and Bufo woodhousei.


Molecular and ultrastructural characterization of the egg capsule of the leech Erpobdella octoculata L.


Movement of triglycerides and monoglycerides across the intestine of Ascaris suum.


Movement of cholesterol and beta-sitosterol across the intestine of Ascaris suum.


The electrogenic sodium pump and membrane potential of identified neurones in Helix aspersa.


Cardiac response to diving in wild ducks.


On the electrical gradient across the gill of the sea water-adapted eel.


Effects of prolonged diving anoxia on the turtle, Pseudemys scripta elegans.


Effects of cytochalasin B and other agents of insulin secretion from Saurian islets.


The effects of nerve transection in walking legs of lobster on acetylcholine, acetylcholinesterase, choline acetyltransferase, adenosine triphosphatase and protein composition.


Osmotic responses of spiny dogfish (Squalus acanthias L.) embryos to temperature and salinity stress.


Body temperatures in the Tasmanian devil, Sarcophilus harrisii (Harsupialia: Dasyuridae).


Effects of calcium and magnesium on facilitation of a unitary synaptic potential in neuron R15 of Aplysia.


The osmolarity of the fluid secreted by the malpighian tubules of Carausius morosus.


The effect of vitamine B6 on alanine transport in turtle intestine.


The prandial state and the glycaemic and lipolytic responses of Gallus domesticus to catecholamines and glucagon.


Ionic distribution and fluxes in holothurian tissues.


A saline transfusion technique for crayfish CNS studies.


Studies on the fibre types of the guinea pig masticatory muscles.


Volume regulation in Nereis diversicolor--I. The steady state.


Volume regulation in Nereis diversicolor--II. The effect of calcium.


Volume regulation in Nereis diversicolor--III. Adaptation to a reduced salinity.


Vibration  detection by the macula neglecta of sharks.


Urea, inulin and para-amino-hippuric acid (PAH) excretion by the gills of the eel, Anguilla anguilla L.


Salt gland secretion in a mangrove monitor lizard.


Bone ash, duodenal 45Ca absorption and calcium-binding protein in chicks fed lathyrogens.


Changes in activity in a freshwater clam in response to oxygen concentration.


High salinity adaptation of ion transport and epidermal lipids of frog skin.


The water balance response of the pelvic "patch" of Bufo punctatus and Bufo boreas;.


Interorgan transport of lipids in the purple sea urchin, Strongylocentrotus purpuratus.


Diurnal patterns of ammonia release in marine and terrestrial isopods.


Responses from tactile receptors in the antenna of the spiny lobster Panulirus japonicus.


Erthrocyte glucose-6-phosphate dehydrogenase deficiency-a pharmacogenetic prototype.


Halazepam in anxious outpatients: a controlled study.


Effects of continuous infusion of cardiotonic agents on arterial cations and glucose in dogs.


Double-blind comparison of the respiratory effects of parenteral lorazepam and diazepam in patients with chronic obstructive lung disease. The effects on the respiratory function of two tranquillising drugs, lorazepam and diazepam, have been compared in 20 patients with chronic obstructive lung disease. Both drugs induce a respiratory depression (decrease in tidal volume and minute ventilation with acceleration of the respiratory frequency) with slight respiratory acidosis, but lorazepam causes no significant hypoxemia and has a shorter duration of action than diazepam. Nevertheless, if tranquillisers are indicated in such patients, they have to be used with care.


Patient acceptance as a factor in the effectiveness of treatment: an open assessment of potassium clorazepate ('Tranxene') and lorazepam in anxiety. Two new benzodiazepines, potassium clorazepate (15 mg. at night), and lorazepam (1 mg. t.d.s.) were compared in 50 patients presenting with anxiety in general practice. The response to treatment was assessed clinically and for patient acceptance. The single-dose regime of potassium clorazepate was significantly better, both for clinical effectiveness and patient acceptance. In the potassium clorazepate treated group there were significantly fewer side-effects. In particular, sedation was a problem with lorazepam, 6 patients stopping treatment and 8 voluntarily reducing the dose. Only 2 patients stopped treatment because of the sedative effect of potassium clorazepate. The sedative effects also show qualitative differences: in patients who completed the assessment lorazepam caused mainly daylong sedation and potassium clorazepate mid-day sedation, and when asked for their opinion on the value of sedation, only the potassium clorazepate group considered it to be beneficial.


Studies of the fate of metabolites and analogs of probenecid. The significance of metabolic sites, especially lack of ring hydroxylation.


Physiologic disposition and metabolic fate of a new anti-inflammatory agent, cis-5-fluro-2-methyl-1-(p-(methylsulfinyl)-benzylidenyl)-indene-3-acetic acid in the rat, dog, rhesus monkey, and man.


Metabolic fate of O2,2'-cyclocytidine.

Metabolic fate of O2,2'-cyclocytidine.


Studies of 9-beta-D-arabinofuranosyladenine in man.


Seclazone: biotransformation during absorption in the rat, binding in plasma, and disappearance from plasma in the dog.


New potent modifiers of liver microsomal drug metabolism: 1-arylimidazoles.


The rate of pentobarbital and acetanilide metabolism by liver microsomes: a function of lipid peroxidation and degradation of cytochrome P-450 heme.


A sensitive radioactive assay for hexobarbital hydroxylase in hepatic microsomes.


Physiological disposition and urinary metabolites of cyproheptadine in the dog, rat, and cat.


Isolation and stereochemical identification of a metabolite of naltrexone from human urine.


The in vitro metabolism of 2-hydroxynicotinic acid in human erythrocytes and platelets.


The radiosynthesis, percutaneous absorption, and metabolism of the wound-healing agent calcium acexamate in the rat.


Metabolism of xenobiotics by the isolated perfused lung. Comparison with in vitro incubations.


Biliary excretion of diazepam in the rat.


A comparative study of xenobiotic-metabolizing enzymes in liver and intestine of various animal species.


Involuntary movements not due to parkinsonism.


beta-adrenoreceptor blocking drugs in hypertension.


Effects of various barbiturates on hepatic microsomal enzymes. A comparative study.


Use of beta-adrenoreceptor blocking drugs in hypertension.


Dibenamine impairment of rat hepatic microsomal enzymes and its relation to hepatotoxicity induced by CCl4 and dimethylnitrosamine.


Studies on irreversible binding of radioactivity from (14C)halothane to rat hepatic microsomal lipids and protein.


Presiding chairman's address to the opening of the Annual Scientific Conference of the Association of the Physicians of East Africa held in Dar es Salaam in June 1974. Medicine and progress.


The urinary excretion profile of naltrexone and metabolites in man.


The effect of diazepam on the metabolism of methadone by the liver of methadone-dependent rats.


The role of ionization in the N-demethylation of some N,N-dimethylamines.


Normal values of plasma gamma-glutamyl transpeptidase in children.


The distribution of malate NADP dehydrogenase in adult, fetal and neoplastic tissues of the rat.


Antilymphocytic antibodies and marrow transplantation. IV. Comparison of the effects of antibody fragments directed against immunoglobulin or lymphocyte antigens on acute secondary disease.


The use of cell separation techniques and isantibody to host antigens in the treatent of severe combined immunodeficiency disease with HL-A incompatible maternal marrow.


Hepatic microsomal mixed-function oxidase activity of several marine species from coastal Maine.


Clinical use of corticosteroids in renal disease.


Fetal hepatic drug metabolism in the nonhuman primate, Macaca arctoides.


Deuterium and tritium isotope effects on the microsomal N-demethylation of ethylmorphine.


The interaction of foreign chemical compounds with the glucuronidation of estrogens in vitro.


Public relations and patient rights.

Public relations and patient rights.


In extended care facilities: reactivation programs cut nursing care costs.


Physician and trustee must unsure competent medical care.


Heavy cost for hospitals: the toll of equipment replacement.


A new skill for nursing homes: the handling of grievances.


Pharmacokinetic studies of the disposition of secobarbital in the dog.


Food service: the challenge of cost control.


Editorial: Let's get the real culprits.

Editorial: Let's get the real culprits.


Implications for inservice education: is it legal to reproduce this page?


Histochemical studies of the rat epididymis after treatment with alpha-chlorohydrin (U-5897). Histochemical studies of the rat epididymis after treatment with alp ha-chlorohydrin (U-5897) are presented.  14 sexually mature male rats received either daily subcutaneous injections of 50 mg U-5907/kg body weight or distilled water for 20 days.  The animals were sacrificed the day following the last injection.  U-5897 induced temporary sterility as demonstrated by blocked transport of spermatozoa, and spermatogenic cells eliminated from the spermatogenic epithelium which became blocked in the caudal part of the epididymis.  This resulted in the distension of the segment of the distal part of the epididymal duct and to the thinning of the epithelium which lined the altered segment.  Alkaline and acid phosphatases, nonspecific esterases, succinate and glucose-6-phosphate dehydrogenases and reduced nicotinamide-adenine dinucleotide tetrazolium reductase in the unchanged part of the epididymal duct were comparable to  control rats whereas the altered part of the epididymis showed these activities to much weaker degrees or to be absent altogether.


Effect of alpha-adrenergic blocking agents on blood cholesterol triglyceride & liver cholesterol biosynthesis in rat.


Pharmacology of neural control.

Pharmacology of neural control.


The treatment of phenothiazine-induced tachycardia by propranolol.


Tryptophan-dehydrobutyrine diketopiperazine, a metabolite of Streptomyces spectabilis.


A new antibiotic, XK-46.

A new antibiotic, XK-46.


In vitro activity of kitasamycin against gram-positive cocci.


In vitro activity of BL-S217, a new cephalosporin antibiotic.


Glucose metabolism in Neisseria gonorrhoeae. The metabolism of glucose was examined in several clinical isolates of Neisseria gonorrhoeae. Radiorespirometric studies revealed that growing cells metabolized glucose by a combination on the Entner-Doudoroff and pentose phosphate pathways. A portion of the glyceraldehyde-3-phosphate formed via the Entner-Doudoroff pathway was recycled by conversion to glucose-6-phosphate. Subsequent catabolism of this glucose-6-phosphate by either the Entner-Doudoroff or pentose phosphate pathways yielded CO(2) from the original C6 of glucose. Enzyme analyses confirmed the presence of all enzymes of the Entner-Doudoroff, pentose phosphate, and Embden-Meyerhof-Parnas pathways. There was always a high specific activity of glucose-6-phosphate dehydrogenase (EC 1.1.1.49) relative to that of 6-phosphogluconate dehydrogenase (EC 1.1.1.44). The glucose-6-phosphate dehydrogenase utilized either nicotinamide adenine dinucleotide phosphate or nicotinamide adenine dinucleotide as electron acceptor. Acetate was the only detectable nongaseous end product of glucose metabolism. Following the disappearance of glucose, acetate was metabolized by the tricarboxylic acid cycle as evidenced by the preferential oxidation of [1-(14)C]acetate over that of [2-(14)C]acetate. When an aerobically grown log-phase culture was subjected to anaerobic conditions, lactate and acetate were formed from glucose. Radiorespirometric studies showed that under these conditions, glucose was dissimilated entirely by the Entner-Doudoroff pathway. Further studies determined that this anaerobic dissimilation of glucose was not growth dependent.


Regulation of nitrogen fixation in Klebsiella pneumoniae: evidence for a role of glutamine synthetase as a regulator of nitrogenase synthesis. Mutations causing constitutive synthesis of glutamine synthetase (GlnC(-) phenotype) were transferred from Klebsiella aerogenes into Klebsiella pneumoniae by P1-mediated transduction. Such GlnC(-) strains of K. pneumoniae have constitutive levels of glutamine synthetase. Two of three GlnC(-) strains of K. pneumoniae studied, each containing independently isolated mutations that confer the GlnC(-) phenotype, continue to synthesize nitrogenase in the presence of NH(4) (+). One strain, KP5069, produces 30% as much nitrogenase when grown in the presence of 15 mM NH(4) (+) as in its absence. The GlnC(-) phenotype allows the synthesis of nitrogenase to continue under conditions that completely repress nitrogenase synthesis in the wild-type strain. Glutamine auxotrophs of K. pneumoniae, that do not produce catalytically active glutamine synthetase, are unable to synthesize nitrogenase during nitrogen limited growth. Complementation of K. pneumoniae Gln(-) strains by an Escherichia coli episome (F'133) simultaneously restores glutamine synthetase activity and the ability to synthesize nitrogenase. These results indicate a role for glutamine synthetase as a positive control element for nitrogen fixation in K. pneumoniae.


Stimulation of transport into Escherichia coli membrane vesicles by internally generated reduced nictotinamide adenine dinucleotide. Membrane vesicles containing internal alcohol dehydrogenase and nicotinamide adenine dinucleotide (NAD) were prepared from Escherichia coli ML308-225. Upon the addition of ethanol, these vesicles respired and transported several amino acids. Transport rates driven by internally generated reduced NAD (NADH) were about 60 to 80% of that stimulated by d-lactate. This transport was inhibited by cyanide and anaerobiosis. Ferricyanide, a nonpermeable electron acceptor, inhibited transport stimulated by external NADH, but not that stimulated by internally generated NADH.


Some social and political ramifications of utilizing non-physicians as chemotherapists.


Primary structure of the N-terminal portion of D-amino acid oxidase.


Isolation of a trans-dominant histidase-negative mutant of Salmonella typhimurium. A mutation of Salmonella typhimurium was obtained that results in the failure of cells to synthesize the enzyme l-histidine ammonia-lyase (histidase). The mutation mapped within the hutH gene and in merodiploid strains was dominant over the wild-type allele. Extracts from cells bearing the trans-dominant histidase-negative allele were shown to contain material that reacts immunologically with antiserum against purified wild-type histidase. It is proposed that the trans-dominant allele results in the synthesis of defective histidase subunits that can combine with, and partially inactivate, wild-type histidase subunits. This subunit mixing presumably does occur, as the enzyme synthesized in a hybrid merodiploid strain is abnormally heat sensitive.


Ferredoxin from a blue-green alga, Aphanothece sacrum (Suringar) Okada.


Heart rate feedback and the management of cardiac neurosis.


The physician's assistant as a buffer in the delivery of primary care.


Parameters in determining the specific activity of the succinate dehydrogenase-coenzyme Q10 reductase in mitochondria of leucocytes.


Proceedings: Effects of the iontophoretic application of bicuculline on the receptive field properties of simple cells in the visual cortex of the cat.


Proceedings: The efferent pathway of the reflex increase in heart rate produced by stimulation of left arterial receptors.


Psychological predictors of sudden death in myocardial infarction.


Comparative resistance to insecticides in populations of three sympatric species of mosquitoes in the Coachella Valley of California.


Experimental rearing of Chagasia bathana (Dyar) using induced mating, and description of the egg stage (Diptera: Cullicidae).


Some aspects on the transmission of hepatitis B antigen; model experiments by mosquitoes with murine hepatitis virus.


Studies on monoamine oxidase (report XXIV). Effect of harmine on monoamine oxidase.


A ganglion blocking action of toxic substances, IS-toxin and surugatoxin, from the Japanese ivory shell, Babylonia japonica.


Pharmacological features of peripheral vascular beds of beagles.


Inhibition of adrenaline-induced fibrinolysis by alpha-adrenergic blocking agents in the rat.


A comparative study on the mechanism of action of morphine on gastric acid secretion in dogs.


Studies on monoamine oxidase. (Report XXV). Effects of alcohols on beef liver mitochondrial monoamine oxidase.


The role of transcortin in glucocorticoid mediated enzyme induction: tyrosine aminotransferase induction in hepatoma tissue culture cells.


Mammillary body fluorescence changes following septal or hippocampal lesions in the rat.


Clinical trials of topical fluorides.

Clinical trials of topical fluorides.


An analysis of the gross behavioural changes induced by tetraethylammonium and a comparison with other substances affecting the autonomic and central nervous system after inraventricular inejctions to conscious cats.


The asexual and sexual circadian rhythms of Plasmodium vinckei chabaudi, of P. berghei and of P. gallinaceum.


Hypertension.

Hypertension.


Vasodilators..

Vasodilators..


Thyroid diseases.

Thyroid diseases.


Epilepsy.

Epilepsy.


Depressive disorders.

Depressive disorders.


Schistosoma mansoni. Sir Patrick Manson, 1844-1922.


Proceedings: Dental plaque formation in relation to type of carbohydrate.


Bioluminescence.

Bioluminescence.


Ion specificity of the aequorin luminescent reaction.


Sedative effects on physiological and psychological measures in anxious patients.


Clinical comparison of anxiolytic drug therapy.


Clinical and biochemical comparison of clorazepate and diazepam.


Relationship between mood and digit-symbol performance in hospitalized patients with functional psychiatric disorders.


A comment on treatment analogues for phobic anxiety states.


Ovarian hormonogenesis and its alteration in the cystic disease. II(nd note).


Metabolism of pronethalol in the rat, the guinea pig and the mouse.


Histological and histochemical studies on effects of some storage solutions on human split thickness skin grafts.


Effect of levamisole on the development of Breinlia sergenti in mosquitoes.


An ultrastructural study on spicule formation in the pennatulid colony Renilla reniformis.


Sea wasp (Chironex fleckeri) antivenene: neutralizing potency against the venom of three other jellyfish species.


The toxic effect of cucumarioside C from Cucumaria fraudatrix on early embryogenesis of the sea urchin.


Sarcophine--a new toxin from the soft coral Sarcophyton glaucum (Alcyonaria).


Differential blockade of ganglionic transmission by extract from venom gland of black widow spider (Latrodectus mactans).


Effects of sea nettle toxin on glucose transport in hamster intestine.


Vitamin E, selenium, and the membrane-associated drug-metabolizing enzyme system of rat liver.


Substitution of sucrose by lycasin in candy "the roslagen study".


Incidence of dental caries among a group of university students in Turku.


Immunofluorescence studies of extracapillary glomerulonephritis.


Rational use of psychotropic drugs. III. Major tranquilizers.


Double-blind study of flunitrazepam (Ro 5-4200) and Mandrax.


Electroretinogram and the superior cervical ganglion.


Decreased tyrosine transaminase activity in iron deficient rat liver.


Thiambutene and acepromazine as analgesic and preanaesthetic agents in horses and sheep.


Effects of acute and chronic pentobarbitone on the gamma-aminobutyric acid system in rat brain.


Alteration of norepinephrine metabolism by barbiturates.


Enzymic demethylation of a carcinogenic tryptophan metabolite, 8-methoxykynurenic acid.


Examination of phenothiazine-albumin interaction by ultraviolet difference spectrophotometry.


Interactions of beta-receptor blockade and PCO2 levles in the anaesthetized dog.


A comparison of lorazepam and diazepam as oral premedicants for surgery under regional anaesthesia.


Fatty acid synthesis in developing brain.


The cardiovascular effects of intraventricular clonidine and Bay 1470 in conscious hypertensive cats. 1 Intraventricular administration of clonidine (5-30 mug) and an analogue, BAY 1470 (15-30 mug) to conscious renal hypertensive cats produced a fall in mean blood pressure lasting for approximately 3 hours. This fall in blood pressure was accompanied by a marked bradycardia.2 Pretreatment with intraventricular phentolamine (100-200 mug), piperoxan (40-200 mug) or tolazoline (75-200 mug) abolished the cardiovascular effects of intraventricular clonidine (20 mug).3 The cardiovascular effects of intraventricular clonidine (20 mug) were not modified by the pretreatment with either haloperidol (1 mg/kg i.p.) or desmethylimipramine (1 mg/kg i.p.).4 Emesis was observed 1-2 min after the administration of either clonidine (5-20 mug) or BAY 1470 (30 mug). This preceded the cardiovascular actions and was still seen after pretreatment with haloperidol, desmethylimipramine, phentolamine, piperoxan or tolazoline.5 It is concluded that the centrally mediated cardiovascular responses observed after intraventricular administration of small doses of clonidine are due to stimulation of central alpha-adrenoceptors and are independent of central catecholamine uptake mechanisms and dopamine receptors.


Proceedings: Potentiation of picrotoxin-induced convulsions in mice by antidepressants. Specificity of the effect.


Biological actions in vivo and in vitro of two gamma-aminobutyric acid (GABA) analogues: beta-chloro GABA and beta-phenyl GABA. 1 The synthesis of two analogues of gamma-aminobutyric acid (GABA), beta-chloro GABA and beta-phenyl GABA is described.2 The activity of brain GABA aminotransferase was inhibited by beta-chloro GABA (5.7 x 10(-5)M) and beta-phenyl GABA (4.6 x 10(-3)M) in a competitive manner with GABA.3 beta-Chloro GABA exhibited 50% of the inhibitory activity of GABA in blocking the discharge of the crayfish stretch receptor neurone; beta-phenyl GABA had no detectable effect.4 Injection of beta-phenyl GABA (200 mg/kg) into normal or epileptic cats (cobalt) caused the appearance of synchronized slow-wave EEG activity.5 Administration of beta-chloro GABA (200 mg/kg) to epileptic cats (cobalt) produced a temporary diminution or abolition of epileptic discharges while causing no alteration in normal EEG activity.6 beta-Chloro GABA and beta-phenyl GABA had no effect on the concentrations of catecholamines or of amino acids in mouse brain.7 The results suggest that both beta-chloro GABA and beta-phenyl GABA may pass the blood-brain barrier.


Proceedings: Synaptosomal exchange of gamma-aminobutyric acid (GABA) can simulate high affinity uptake.


Proceedings: On the release of accumulated (3-H)-gamma-aminobutyric acid (GABA) from isolated rat superior cervical ganglia.


Proceedings: Anticonvulsive action of homotaurine and taurine.


Proceedings: Brain levels of the potent analgesic etorphine in rats and their functional significance.


Proceedings: Dihydropteridine reductase: enzyme characteristics, regional distribution and ontogenetic development.


Proceedings: Definition of the antagonistic action of burimamide and metiamide on the positive inotropic effect of histamine in isolated heart preparations.


Brain histamine alterations after hypothalamic isolation.


Commentary. Similarities of T cell function in cell-mediated immunity and antibody production.


Conversion of type III pneumococcal polysaccharide low responders to high responders by immunization with a thymus-dependent form of antigen.


Interaction between thymocytes and lymph-node cells in the graft-versus-host response: evidence for a synergistic and an antagonistic activity mediated by mouse thymocytes.


Transfer factor: therapy in patients with deficient cell-mediated immunity and deficient antibody-mediated immunity.


Survey of the relative prevalence of potential yellow fever vectors in north-west Nigeria. The yellow fever epidemic in Nigeria in 1969-70 emphasized the lack of data concerning the possible importance of Aedes aegypti and other Stegomyia mosquitos as vectors. An entomological survey was therefore undertaken in September 1973 in 6 areas in the north-west of Nigeria to determine the prevalence of Stegomyia populations in the villages. An examination of over 6 700 water pots showed that 11-53% contained A. aegypti larvae, and in some areas larvae of A. vittatus were found in up to 18% of pots. In villages in the relatively dry Sudan savanna neither leaf axils nor tree-holes were important Stegomyia larval habitats, but in the more southern Kontagora area of the wetter northern Guinea savanna, these habitats were probably important breeding sites. In the early evening the most abundant man-biting mosquito in the villages was A. aegypti. A. vittatus was also caught at bait in some villages. It was concluded that the only potential yellow fever vectors in the area were A. aegypti and A. vittatus. There were large populations of A. aegypti, closely associated with man, in all the areas surveyed, but they should not present a risk of yellow fever transmission unless the disease were to be introduced into the area by man, or unless virus reservoirs, such as monkeys, were also present. Although monkeys were common in the Kontagora area they were rare in the Sudan savanna.


Biochemical and immunological studies on isolated brush border membranes of human kidney cortex and their membrane surface proteins.


The graft versus host reaction in man after bone marrow transplantation: pathology, pathogenesis, clinical features, and implication.


Excitatory and inhibitory effects of noradrenaline on the isolated guinea-pig vas deferens.


Ornade and anticholinergic toxicity: hypertension, hallucinations, and arrhythmias.


The pharmacology of MS 222 (ethyl-m-aminobenzoate) in Squalus acanthias.


Visual evoked potentials as a measure of drug effects on arousal in the rabbit.


The ionic mechanism of action of GABA and L-glutamate on a crustacean striated muscle (vas deferens of the crayfish).


Beta blockers in psychiatric illness.

Beta blockers in psychiatric illness.


Plasma renin response to acute blockade of angiotensin II in the anaesthetized rat.


Cyclid AMP and catabolite repression in yeasts,  In Schizosaccharomyces pombe glucose lowers both intracellular adenosine 3':5'-monophosphate levels and the activity of catabolite-sensitive enzymes.


Cardiovascular effects of beta-adrenoreceptor blocking agents after intracerebroventricular administration in conscious normotensive cats.


Central adrenoreceptors and the control of arterial blood pressure.


Non-catechol phenylethanolamines: agonistic and antagonistic actions on beta-adrenoreceptors in isolated tissues from the guinea-pig.


Bioluminescence: Chemical Aspects.

Bioluminescence: Chemical Aspects.


Personnel development for the Illinois emergency medical services system.


Factors influencing the circadian periodicity of plasma corticosteroid levels.


Illinois emergency medical service system: status report III (July, 1974).


Water fluoridation: a choice for the community.


EKG of the month.

EKG of the month.


The view box.

The view box.


Synthesis and properties of arginine oligopeptides.


Study on vitamin A effects upon lipid peroxidation in rat liver.


Deficiency of activity of succinate dehydrogenase-coenzyme Q10 reductase in leucoytes from patiens with essential hypertension.


Fertility after treatment of bilateral cryptorchidism.


The utilization of glucose in the brain slices during uraemia.


Japanese encephalitis virus surveillance in Taiwan. II. Isolations from mosquitoes and bats in Taipei area 1969-1970.


Design of agents for stimulation of neostriatal dopaminergic mechanisms.


Inhibition of human platelet 5-hydroxytryptamine uptake by beta-phenethylamine derivatives.


Interaction of phenobarbitone and ethanol in mice studied from dose-response curves and drug concentrations in blood.


Effect of butriptyline on the brain uptake mechanisms for noradrenaline and 5-hydroxytryptamine.


Effect of acute and chronic treatment with practolol on cardiovascular responses in the pithed rat.


An analysis of the inter-subject variation in the metabolism of pentazocine.


Inhibition by oxalomalate of isocitrate (NADP+) dehydrogenase.


A molecular orbital study of tissue selectivity in beta-adrenoceptor blocking agents.


Improved parameter estimates in drug-protein binding studies by non-linear regression.


Influence of some tricyclic antidepressive drugs on the uptake of 5-hydroxytryptamine by rat blood platelets.


Increased concentration of homovanillic acid in the brains of infant rabbits after administration of haloperidol to their nursing mothers.


The fate of [14-c] lactose and administered into the lungs of rats and monkeys.


On the mechanism of the hyperthermia induced by amphetamine in the rat.


Evidence against the involvement of false neurotransmitters in tolerance to amphetamine-induced hyperthermia in the rat.


The influence of atrial temperature on the in vitro selectivity of isoprenaline for atria and of orciprenaline for trachea.


Triparanol-effected accumulation of desmosterol in oestrone-induced hypocholesterolaemia.


Inhibition of gastric acid secretion in the rat by orally-administered synthetic prostaglandin analogues: enantiomorphs of 15-hydroxy-15-methyl-9-oxoprostanoic acid.


Antispasmodic action of quaternary compounds administered orally.


A human plasma fraction with anti-inflammatory but without either analgesic or antipyretic properties.


A new source of anabasine.

A new source of anabasine.


Anomalous behavior of some hydroflumethiazide crystal samples.


The antimicrobial efficiencies of contact lens solutions.


Some solution properties of pentagastrin and angiotensin: aggregation of pentagastrin.


Aggregation of antihistamines in aqueous solution: micellar properties of some diphenylmethane derivatives.


An analogue computer simulation of drug dissolution.


The actions of some anticholinesterase drugs on skeletal muscle in culture.


Neuromuscular and other blocking actions of a new series of mono and bisquaternary aza steroids.


The effects of a human plasma fraction on carrageenan-induced paw oedema in the rat.


An ESR titration technique for the determination of accessible tyrosine and tryptophan residues in globular proteins.


Gas-liquid chromatographic estimation of lignocaine, ethylglycylxylidide, glycylxylidide and 4-hydroxyxylidine in plasma and urine.


A gas chromatographic determination of carbenoxolone in human serum.


Evaluation of the antibacterial activity of contact lens solutions.


The production of extracellular lipids by Pseudomonas Aeruginosa NCTC 2000 in stationary liquid media containing macrogols.


The use of desipramine in studies of noradrenergic nerve function.


Interactive effect of (plus or minus)-fenfluramine and (+)-amphetamine on feeding in rats.


Effects of the dopamine-beta-hydroxylase inhibitor FLA 63 on the kinetics of elimination of amphetamine in the rat.


Correlation (+)-amphetamine lethality and hepatic metabolism.


Long-lasting reduction of brain 5-hydroxytryptamine concentration by 3-chloramphetamine and 4-chloroamphetamine in iprindole-treated rats.


Binding of (+)- and (minus)-delta-1-tetrahydrocannabinols and (minus)-7-hydroxy-delta-1-tetrahydrocannabinol to blood cells and plasma proteins in man.


Inhibition of eosinophil chemotaxis by the antagonist of slow reacting substance of anaphylaxis--compound FPL 55712.


Characterization of phenobarbitone samples.


Sensitivity analysis and validation of stimulation models.


Pharmacological studies of the rabbit and human renal pelvis.


Correlations between oral hygiene status, ginigival health and dental caries in school children.


The effect of ketamine upon depolarizing and non-depolarizing neuromuscular blockade in rabbit.


Evidence against a causal relationship between increase in c-AMP and induction of tyrosine hydroxylase in the rat adrenal medulla.


Frostbite.

Frostbite.


Hypothermia in old age.

Hypothermia in old age.


Retained anticaries protection from topically applied acidulated phosphate-fluoride: 30- and 36-month post-treatment effects.


Prolintane in debility and fatigue. Report of a trial among college students in Cardiff.


Vitamins and drug metabolism with particular reference to vitamin C.


Effect of prednisolone and cocaine on red cell volume-increased by pneumococcal endotoxin.


Drugs and appetite.

Drugs and appetite.


Further observations of the effect of furosemide on mesenteric blood flow in dogs.


Effect of metiamide, a histamine antagonist of H2-receptors, on acid secretion of isolated canine stomach perfused with homologous blood.


Effect of chronic administration of centrally acting compounds on chloramphenicol metabolism in schizophrenic patients.


Patient management seminars.

Patient management seminars.


"Should physical therapists become physician's assistants?".


Practical points regarding routine determination of gamma-glutamyl transferase (gamma-GT) in serum with a kinetic method at 37 degrees C.


Public health nursing trends abroad.

Public health nursing trends abroad.


Stinging coral (Millepora tenera) toxin:  a comparison of crude extracts with isolated nematocyst extracts.


Toxic and immunological properties of stinging coral toxin.


Endemic fluorosis in Ceylon.

Endemic fluorosis in Ceylon.


Isolation of arboviruses in Kenya, 1966-1971.


A post-vaccinal inclusion body encephalitis in dogs.


Cinchophen duodenal ulcers in the mouse.


Simplified symptom registration in psychiatric trials: a statistical approach.


Attitudes of primary care physicians in the state of alabama toward physicianassistants: a preliminary report.


A study of flupenthixol decanoate and pipotiazine undecylenate in schizophrenics.


Methemoglobin reductase variability as related to NAD glycohydrolase activity.


The role of the Krebs cycle in the generation of intramitochondrial reducing equivalents for the 11 beta-hydroxylation of deoxycorticosterone in isolated rat adrenal cells.


Regulation of hepatic acetyl coenzyme A carboxylase by phosphorylation and dephosphorylation.


Differential effects of metabolites on the active and inactive forms of hepatic acetyl CoA carboxylase.


The effect of quinolinic acid on the content and distribution of hepatic metabolites.


Protection by some beta-adrenergic blocking agents and other cardiovascular drugs against acute anoxia in isolated guinea-pig atria.


Inhibition by cyanide of the respiratory chain oxidases of Escherichia coli.


Pharmacological studies with a tripeptide, prolyl-leucyl glycine amide.


Effect of predosing animals with immunosuppressive drugs on immune and non-immune induced inflammatory responses.


The enzymes of the hepatobiliary tract: a biochemical and clinical comparison. The "biliary tract" enzymes (leucine aminopeptidase, gamma-glutamyltranspeptidase and 5'-nucleotidase) in serum reflect to varying degrees, obstruction, proliferation, inflammation and neoplasia involving the hepatobiliary duct system. Their use is directed towards two purposes: (1) as non-electrophoretic assays to evaluate the source of an elevated non-specific alkaline phosphatase and (2) to offer greater sensitivity and specificity for space-occuping lesions in the liver. In appropriate clinical states, any of the three enzymes offer these advantages and there is little to chose among them. Selection of the assay to use in the clinical laboratory then becomes based on non-clinical factors, i.e., technical ease, apparent substrate specifities, etc. With these additional factors and despite some shortcomings, our selection is leucine aminopeptidase.


Giant intraabdominal seminoma: case report with a brief review of the literature.


Effects of adrenoceptor-stimulating drugs on the myometrium and cardiovascular system in nonpregnant women.


Control of uterine activity and prevention of premature birth.


The mechanisms of ejaculation.

The mechanisms of ejaculation.


Dwarfism and congenital medullary stenosis (Kenny syndrome).


Connections of the basal ganglia and of the cerebellum.


Quantitative histochemistry of gamma-aminobutyric acid (gaba) in the human substania nigra and globus pallidus.


Fiber connections within the extrapyramidal system.


Functional aspects of, and the role of transmitter in, the basal ganglia.


Piperacetazine in ambulatory chronic schizophrenic patients.


Effects of lorazepam on CNS structures: neurophysiological and behavioral correlations.


Beta-blockade for hypertension.

Beta-blockade for hypertension.


The effect of narcotic analgesics and their antagonists on conditioned avoidance in the rat.


End products of human metabolism as affected by diet and space conditions.


Metabolic controls in precancerous liver--VI. Studies in vivo and in vitro on feedback regulation of delta-aminolevulinic acid synthetase in ethionine-fed rats.


Leukemic transformation of donor spleen cells following their transplantation into superlethally irradiated mice with pre-existing viral leukemia.


Pathophysiological implications of the hepatitis associated antigen (HAA).


Psychiatric sequelae to term birth and induced early and late abortion: a longitudinal study. Three obstetric groups matched for parity, age, color, marital and socioeconomic status, were compared for the psychosocial antecedents to abortion or term delivery and sequelae that did exist.  One group had induced abortion by suction curettage, the second had induced abortion by saline injection, and the third had term delivery.  373 women were interviewed during pregnancy; 211 were included in follow-up after an average of 16 months for abortion patients and 13 months for the term delivery group.  The 373 women received a structured 90-minute interview about family relationships, consort relationships, living arrangements, income, education, religious behavior, sexual behavior, contraceptive knowledge and use, pregnancy history and  desired family size.  The Srole Anomia scale and the Rosenberg Self-Esteem scale were also used.  At the followup interview, similar questions were asked and the two attitude scales were again presented.  The MMPI (Minnesota Multiphasic Personality Inventory) and the SCL (Symptom Check List) were also used.  12% of the 211 follow-up scored higher than 70 on the MMPI for mania and psychopathy scales; these women were evenly divided among the three groups.  SCL scales showed the three groups of patients to be similar after abortion or birth, with the only statistically significant difference being the suction curettage group, which had fewer complaints after abortion than either of the other two  groups.  There was no difference on the anomia or self-esteem scale between pre- or post-procedure for any of the groups.  The data suggest neither great harm nor great benefit with regard to these variables due to either early or late abortion or term birth.  Early abortion by suction curettage was possibly more therapeutic than carrying a pregnancy to term.  There were striking improvements with regard to contraceptive use after the procedure.  71% of each group used effective contraception for more than 12 months after abortion or birth: a 20% increase for the term birth group, a 13% increase for the suction curettage group, and a 26% increase for the saline abortion group.  Among abortion patients, those found to have low self-esteem, low contraceptive knowledge, high alienation, and who delayed in seeking abortion were related to long recovery times, a high MMPI psychopathology scale, and a number of unpleasant  body symptoms post-abortion.  These patients could be predicted with accuracy 32% better than chance alone.


Proceedings: Oxidative phosphorylation in intact chloroplasts.


Blockade of the central hypotensive effect of clonidine by alpha-adrenoreceptor and antagonists in rats, rabbits and dogs.


Proceedings: Control of acetyl-CoA carboxylase activity in isolated rat hepatocytes.


Proceedings: Alpha-adrenergic stimulation of renal gluconeogenesis in isolated kidney tubules.


Proceedings: Catalytic and regulatory properties of different mitochondrial acetoacetyl-CoA acetyltransferases from ox liver.


Proceedings: Cyclic GMP, guanylate cyclase and growth control.


Phosphines as ligands to microsomal cytochrome P-450.


Inhibitory and excitatory beta adrenergic receptors in the vas deferens of guinea pig.


Premedication.

Premedication.


Metabolism of volatile anesthetics.

Metabolism of volatile anesthetics.


Postoperative analgesia.

Postoperative analgesia.


The sympathetic nerve ending.

The sympathetic nerve ending.


The effect of particle size and shape on the flow and failure properties of procaine penicillin powders.


Proceedings: The mechanics of inhibition of growth by some antibacterial agents.


Proceedings: Enhancement of lincomycin activity against E. coli by alcohols.


Proceedings: Methicillin-induced lysis of some methicillin-resistant strains of Staphylococcus aureus.


Proceedings: Inactivation of beta-lactam antibiotics by Pseudomonas aeruginosa.


Proceedings: Disaggregation of compressed tablets.


Proceedings: Granule consolidation during compaction.


Proceedings: A comparative study of the gamma-radiation responses of Bacillus megaterium spores suspended in aqueous solutions of ethanol and ethylene glycol.


Proceedings: Evaluation of some tablet disintegrants and the effect of disintegrant type on the dissolution efficiency/compressional force relation.


Proceedings: The effects of binding agents on granule strength and tablet strength.


Proceedings: The use oa a gelatin hydrolysate to improve the compressibility of paracetamol and phenacetin.


Proceedings: The effect of candicidin on exogenous cholesterol absorption in small laboratory animals.


Proceedings: Factors in the experimental evaluation of calcium glycerophosphates in caries.


Shearing and tensile tests on mixtures of pharmaceutical powders.


Proceedings: Pharmacological studies on the leaves of Azadirachta indica.


Proceedings: Alkaloids of the roots of Erythroxylum monogynum Roxb.


Proceedings: Preliminary studies concerning the metabolism of hyoscine and hyoscyamine in the Solanaceae.


Proceedings: Alkaloids from S. American species of Uncaria (Rubiaceae).


Proceedings: The chemotaxonomic significance of alkaloids in the Naucleeae.


Proceedings: Methylating and demethylating enzymes in Papaver somniferum latex.


Proceedings: The equivalence of oxytetracycline tablets B.P.


Proceedings: Modification of trypsin activity by anti-inflammatory drugs.


Proceedings: A new fluorimetric estimination of oxytetracycline in plasma: comparison with microbiological method.


Proceedings: The effect of hydrocortisone on tryptophan metabolism in the rat.


Proceedings: An examination of the possible contribution of circulating corticosterone to the changes in brain monoamine metabolism during the oestrous cycle, pregnancy and the post-partum period in mice.


Proceedings: The labilization of lecithin liposomes by steroidal anaesthetics: a correlation with anesthetic activity.


Proceedings: Interactions of 4-chlorophenol and phenol with phosphatidylethanolamine monolayers in relation to antibacterial action.


Proceedings: Lysozyme--preservative interactions in eye preparations.


Proceedings: A novel sterility test for chloramphenicol.


Proceedings: Inhibition of cholinesterase activity by two methylacridinium compounds.


Proceedings: The effect of hydrotropic salts on the stability of liquid crystalline systems.


Proceedings: Micellar interactions of bile salts with alkytrimethylammonimum bromides.


Proceedings: Substituted ethoxy groups in anionic surfactants; effect on cmc, area per molecule and micellar counterion binding.


Proceedings: pH-mobility plots for drug suspension systems and the effect of added surface active agents.


Proceedings: Viscometric studies on surface agent solutions and the examination of hydrophobic interactions.


Proceedings: Correlation of phase inversion temperature with stability of oil-in-water emulsions stabilized by (polyoxyethylene)4 cetyl ether.


Proceedings: Viscoelastic and continuous shear properties of Carbopol gels.


Proceedings: The influence of alcohol chain length on the structure and rheology of emulsions stabilized by cetomacrogol/long chain alcohols.


Proceedings: Transit in free films: casting solvent effects.


Proceedings: The relationship between the physical and drug release properties of polyethyleneglycol bases.


A kinetic study of human urinary excretion results for butobarbitone and its metabolites.


Solute migration during granule drying.

Solute migration during granule drying.


The influence of additives on the in vitro release of drugs from hard gelatin capsules.


Application of polarography to the development of a stability-indicating assay method for a new indole derivative and its tablet formulations.


The mechanical properties of some binders used in tableting.


The effects of moisture content and gelatin binding agent on the mechanical and failure properties of an oxytetracycline formulation.


Proceedings: Determination of the flow properties of powders by means of a flow balance.


Proceedings: The effect of the specific surface area of primidone on its tableting properties.


Proceedings: A preparative technique for studying the structure of compacts.


Proceedings: Indentation hardness testing of tablets.


The characterization of the mechanical strength of tablets.


A preliminary evaluation of a mercury intrusion method for assessing film continuity on coated tablets.


Proceedings: Apparent biological half-life values determined by administration of drug by methods other than rapid intravenous injection.


Proceedings: Bioavailability of the quaternary ammonium compound thiazinamium methylsulphate (Multergan) after oral and intramuscular administration.


Proceedings: A mucin model for the in vitro evaluation of mucolytic agents.


Proceedings: Effect of mucin on the bioavailability of tetracycline from the gastro-intestinal tract: in vivo, in vitro correlations.


Proceedings: the effect of food on the in vivo release of propranolol from a PVC matrix tablet in the dog.


Proceedings: The renal clearnace of practolol in man.


Proceedings: Elimination biokinetics of some topically-applied steroids.


Proceedings: Stability of drugs in the presence of pharmaceutical colours.


Proceedings: Catalytic degradation of hydrocortisone disodium phosphate solutions by copper (II) ions.


Proceedings: Mechanism of degradation of 5-bromouracil in aqueous solutions of sodium bisulphite.


Degradation of paracetamol by a Penicillium species.


Proceedings: Kinetics of microagglomeration in liquid suspension.


Proceedings: Self-association of some antihistamines in aqueous solution.


Proceedings: Ortho-effects in structure-activity studies. Shielding of hydroxyl by alkyl groups.


Proceedings: Hydrophobic substituent constants from thin-layer chromatography on polyamide plates.


Proceedings: Intragranular solute migration in a test granulation dried by fluidization and vacuum tumbler drying.


Proceedings: Scale up considerations in fluidized bed granulation: air flow rates and air pressure distribution.


Proceedings: Particle size obtained on disintegration of phenylbutazone tablets and its relation to dissolution rate.


Proceedings: Size analysis of sub-micron particles by centrifugal photosedimentometer.


Proceedings: The use of an image analyser to determine the particle size distribution of salbutamol for use in metered dose inhalation aerosols.


Proceedings: Disposition of disodium cromoglycate administered in three particle sizes.


Proceedings: The effects of some prostaglandins on respiration in the rabbit.


Proceedings: The effect of amantadine on the turnover of catecholamines in the rat brain.


Proceedings: The effect of surfactants on the radiation sensitivity of benzocaine in aqueous solution.


Proceedings: The effect of cosolvents on the sensitivity of benzocaine in aqueous solution to ionizing radiation.


Proceedings: Investigations of solid dispersions of primidone in citric acid.


Proceedings: Some effects of polyvinylpyrrolidone on the solubility and dissolution rate of allopurinol.


Proceedings: The dissolution kinetics of sulphanthiazole form I.


Proceedings: The thermodynamic properties of sulphanthiazole.


Proceedings: Thermodynamic parameters for the solubilizaiton of some steroids by non-ionic surfactants.


Proceedings: A method of testing the applicability of the diffusion layer dissolution method.


Proceedings: Removal of hydrophobic surface coatings from a non-disintegrating hydrophilic solid in a water stream.


Proceedings: The determination of partition coefficient data using the "AKUFVE" method.


Proceedings: Some interactions between fenfluramine and antidepressant drugs: effects on 5-hydroxyindole concentrations in rat brain.


Proceedings: Changes in the concentrations of 5-hydroxytryptamine and noradrenaline in six areas of rat brain during recovery from chronic methylamphetamine administration.


Proceedings: The binding of zinc to human serum proteins.


Proceedings: the effect of blending on the physical properties of acid and alkaline gelatin gels.


Proceedings: Biogenic amines and the anti-nociceptive activity of agents with a non-opiate-structure.


Proceedings: Mechanical properties of gelatin films.


Proceedings: The determination of disulfiram in blood, and of exhaled carbon disulphide using cathode ray polarography.


Proceedings: Sensitivity of R+ strains of Proteus mirabilis to sodium desoxycholate.


Proceedings: R-factor mediated resistance to ultraviolet light.


Proceedings: Trimethoprim resistance in Escherichia coli.


Proceedings: The effect of some antibacterial agents on proton flux across the membrane of Clostridium welchii.


Proceedings: The effect of chloroquine on the growth and viability of Escherichia coli.


Central catecholamine neurons, behavior and neuroleptic drugs: an analysis to understand the involvement of catecholamines in schizophrenia.


The regulation of the noradrenergic neuron.


Regulation of catecholamine-synthesizing enzymes.


Genetic differences in mechanisms involving neuroregulators.


Possible role of catecholamines (CA) in the regulation of adrenal tyrosine hydroxylase (TH).


Dose equivalence of the antipsychotic drugs.


Dopaminergic blocking effects of antipsychotic drugs.


Antipsychotic drugs and catecholamine synapses.


New developments in the study of the mechanism of action of neuroleptics and amphetamine.


The child health associate in a rural setting.


Are there characteristics of the malpractice phenomenon peculiar to the black physician and patient.


Biochemical characterization of the dopamine receptor in the mammalian caudate nucleus.


Antischizophrenic drugs: affinity for muscarinic cholinergic receptor sites in the brain predicts extrapyramidal effects.


Antipsychotic drugs and catecholamine synapses. Summary of the session.


Monoamines, pyrogens and cations: their actions on central control of bodytemperature.


Stimulatory effect of citrate on respiration and energy dependent transhydrogenation reaction in sonic particles.


Molecular geometry and adrenergic drug activity.


The physiological role of taurine in retina: uptake and effect on electroretinogram (ERG).


Teratogenic and other effects produced in mice by norethynodrel and its 3-hydroxymetabolites.


Effects of intraphippocampal administration of GABA on avoidance conditioning in the cat. Behavioral and EEG data.


Glutamine synthetase in the embryonic chick neural retina: the effects of cycloheximide on the conservation of labile templates for enzyme synthesis.


Assembly of lipids and proteins in Escherichia coli membranes.


Aspects on delivery of ear, nose, and throat care to Montana Indians.


Turku sugar studies. I. An intermediate report on the effect of sucrose, fructose and xylitol diets on the caries incidence in man.


Folic acid and vitamin B12: transport and conversion to coenzyme forms.


Amplifying effect for glucocorticoid actions by a new polypeptide from enteric flora.


The cyclic AMP system and drug development.


The regulation of the pentose phosphate cycle in rat liver.


Effect of endotoxin on metabolic regulation in mice.


Slow conformational changes of a Neurospora glutamate dehydrogenase studied by protein fluorescence. 1. The NADP-dependent glutamate dehydrogenase of Neurospora crassa undergoes slow reversible structural transitions, with half-times in the order of a few minutes, between active and inactive states. The inactive state of the enzyme, which predominates at pH values below 7.0, has an intrinsic tryptophan fluorescence 25% lower than that of the active state, which predominates at pH values above 7.6. The inactive state can be activated either by an increase in pH or by addition of activators such as succinate. 2. The kinetics of the slow transitions that follow activating and inactivating rapid changes in conditions have been monitored by measurements of protein fluorescence. The results show that the slow reversible conformational change detected by the change in fluorescence is the rate-limiting process for enzyme activation and inactivation. 3. In both directions this conformational change follows apparent first-order kinetics and the rate constant is independent of protein concentration. These kinetics and published measurements of molecular weight are indicative of an isomerization process. 4. In both directions the changes show a large energy of activation and a large positive entropy of activation, consistent with a considerable disturbance of conformation in the transition state. 5. Comparisons of the fluorescence emission spectra of the active and inactive states indicate that the difference in fluorescence is produced by quenching, possibly intramolecular, in the inactive conformation. Iodide ions cause similar quenching. 6. In some mutationally altered forms of the enzyme comparable but modified conformational changes can be followed by protein fluorescence.


The enthalpy change for the reduction of nicotinamide--adenine dinucleotide. The heat of the reaction NAD(+)+propan-2-ol=NADH+acetone+H(+) was determined to be 42.5+/-0.6kJ/mol (10.17+/-0.15kcal/mol) from equilibrium measurements at 9-42 degrees C catalysed by yeast alcohol dehydrogenase. With the aid of thermochemical data for acetone and propan-2-ol the values of DeltaH=-29.2kJ/mol (-6.99kcal/mol) and DeltaG(0)=22.1kJ/mol (5.28kcal/mol) are derived for the reduction of NAD (NAD(+)+H(2)=NADH+H(+)). These values are consistent with analogous but less accurate data for the ethanol-acetaldehyde reaction. Thermodynamic data for the reduction of NAD and NADP are summarized.


Extraction, partial purification and properties of obelin, the calcium-activated luminescent protein from the hydroid Obelia geniculata. 1. The Ca(2+)-activated luminescent protein obelin was extracted from the hydroid Obelia geniculata. 2. After the addition of a large excess of calcium (greater than 5mm) a peak in the rate of luminescence occurred within 100ms, followed by an exponential decay (k=2.8s(-1)). The obelin activity (light emitted) was measured by the peak height or by the total number of counts recorded on a scalar in the first 10s after addition of Ca(2+). 3. After an overnight extraction in 40mm-EDTA-200mm-Tris-HCl, pH7.0, 7.2x10(11) counts were obtained from 186g of wet hydroids. 4. The stability of the crude extracts was dependent on pH, being optimal at pH7.0. 5. Obelin could be purified threefold with a yield of 69% by selecting the protein precipitated between 60%- and 100%-saturated (NH(4))(2)SO(4). The precipitate could be stored for at least 6 months as a suspension in 40mm-EDTA+saturated (NH(4))(2)SO(4), pH7.0, frozen at -70 degrees C with a recovery of 95-100%. 6. Luminescence was also stimulated by Sr(2+). However, obelin appeared to have a lower affinity for Sr(2+) than for Ca(2+). Mg(2+) inhibited Ca(2+)-activated luminescence. 7. Obelin could be used to assay as little as 50pmol of Ca(2+) in a final volume of 1ml. 8. At pH7.0 in Ca(2+)-EGTA [ethanedioxybis(ethylamine)tetra-acetate] buffers the rate of obelin luminescence was proportional to the square of the free Ca(2+) concentration in the presence and absence of 1 and 10mm-Mg(2+). Over the range 0.1-10mum-Ca(2+) less than 0.03% of the obelin was consumed/s. 9. In order to use obelin to study free ionized Ca(2+) concentrations similar to those found inside cells in the presence of 10mm-Mg(2+) a minimum of 10(8) counts were required. A total of 10(12) counts can be readily extracted from about 200g of wet hydroids. Thus a sufficient quantity of an aequorin-like calcium-activated luminescent protein should now be available to workers in the United Kingdom in order to carry out physiological experiments.


The reconstitution of microsomal redox chains. A comparitive analysis of the effectiveness of membrane self-assembly and template binding of electron carriers. A comparative analysis was made of the effectiveness of three methods for the reconstitution of microsomal electron-transfer chains, namely, self-assembly, incorporation of electron carriers into liposomes (non-specific template) and incorporation into ;ghosts' of microsomal vesicles (specific template). It was shown that when the ;ghosts' of the microsomal vesicles were used as a specific template extra cytochrome b(5) and NADH-specific flavoprotein were incorporated into them, but cytochrome P-450 and NADPH-specific flavoprotein were not incorporated into the membrane. As a result of the self-assembly and incorporation into liposomes all the electron carriers were present in the reconstituted membrane. Cytochrome P-450 reactivation took place and the inactive form, cytochrome P-420, was converted into the active form, cytochrome P-450. Of the four enzyme hydroxylation systems studied, i.e. NADPH- and NADH-dependent p-hydroxylation of aniline, and NADPH- and NADH-dependent N-demethylation of dimethylaniline, only the NADH-dependent demethylation of dimethylaniline (60% of the initial value) and NADH-dependent p-hydroxylation of aniline (30% of the initial value) were reconstituted by self-assembly. NADPH oxidase and NADH oxidase activities were only properly reconstituted by self-assembly and incorporation into liposomes. In contrast, the NADPH-specific system of peroxidation of unsaturated fatty acids was reconstituted by specific template-binding.


Changes in respiratory activities during the cell-cycle of the fission yeast Schizosaccharomyces pompe 972h--growing in the presence of glycerol. 1. The specific activities of cytochrome c oxidase, catalase, succinate dehydrogenase, succinate-cytochrome c oxidoreductase, NADH-cytochrome c oxidoreductase, and NADPH-cytochrome c oxidoreductase in mid-exponential-phase batch cultures of glycerol-grown Schizosaccharomyces pombe indicated that the organisms were catabolite-de-repressed. 2. In cultures growing synchronously in the presence of glycerol as sole carbon source, the respiration rate showed two abrupt increases at about 0.45 and 0.95 of the cell-cycle and remained constant in the periods between successive rises. 3. Catalase, succinate dehydrogenase, NADH-cytochrome c oxidoreductase and acid p-nitrophenyl-phosphatase all showed peak patterns of expression in synchronous cultures. 4. Cytochrome c oxidase and cytochromes a+a(3) both showed step patterns of expression with two rises per cell-cycle. 5. Cytochromes c(548), b(554) and b(560) all followed similar time-courses in step patterns of expression, but these were distinct from, and more complex than, that of cytochromes a+a(3). 6. These results are compared with those previously obtained with glucose-grown cultures, and the part played by catabolite repression in the expression of respiratory activities in the cell-cycle is assessed.


Putrescine catabolism in mammalian brain. In contrast with putrescine (1,4-diaminobutane), which is a substrate of diamine oxidase, monoacetylputrescine is oxidatively deaminated both in vitro and in vivo by monoamine oxidase. The product of this reaction is N-acetyl-gamma-aminobutyrate. The existence of a degradative pathway in mammalian brain for putrescine is shown, which comprises acetylation of putrescine, oxidative deamination of monoacetylputrescine to N-acetyl-gamma-aminobutyrate, transformation of N-acetyl-gamma-aminobutyrate to gamma-aminobutyrate and degradation of gamma-aminobutyrate to CO(2) via the tricarboxylic acid cycle.


Energy-linked reduction of nicotinamide--adenine dinucleotide in membranes derived from normal and various respiratory-deficient mutant strains of Escherichia coli K12. 1. Assay conditions are described for the ATP-dependent, uncoupler-sensitive, energy-linked reduction of NAD(+) by succinate, dl-alpha-glycerophosphate or d-lactate in membranes from aerobically grown Escherichia coli. 2. The reaction may be demonstrated in electron-transport particles (ET particles) from cells grown in glycerol, but not in depleted particles washed in low-ionic-strength buffer, or in ET particles from cells grown in glucose. 3. The latter two classes of particles have low specific activities of ATPase (adenosine triphosphatase), succinate dehydrogenase, dl-alpha-glycerophosphate dehydrogenase and d-lactate dehydrogenase relative to undepleted ET particles from cells grown in glycerol. 4. Reconstitution of energy-linked NAD(+) reduction in particles from cells grown in glucose was done by: (a) addition of the high-speed supernatant fraction from sonicates of the same cells; (b) addition of a protein fraction, precipitated by (NH(4))(2)SO(4) from this supernatant, or (c) addition of an (NH(4))(2)SO(4)-precipitated fraction from the low-ionic-strength wash of particles from cells grown in glycerol. 5. The use of (NH(4))(2)SO(4)-precipitated fractions from ATPase- or succinate dehydrogenase-deficient mutants grown in glycerol in the above reconstitution indicated that failure to demonstrate the reaction in particles from cells grown in glucose was a result of inadequate activities of appropriate dehydrogenases, rather than of ATPase. 6. Energy-linked NAD(+) reduction could be demonstrated in particles from a ubiquinone-deficient mutant only after restoration of NADH oxidase activity by adding ubiquinone-1. 7. The measured rate of the energy-linked reaction in particles from a haem-deficient mutant, however, was not stimulated after the ATP- and haematin-dependent acquisition of functional cytochromes. 8. Results are interpreted as evidence of the ubiquinone-dependent, but cytochrome-independent, nature of the site I region of the respiratory chain in E. coli.


Hormonal control of liver regeneration.

Hormonal control of liver regeneration. Two peaks in cyclic AMP production in rat livers 4 and 12h after partial hepatectomy (MacManus et al., 1972) were confirmed and a third peak established at 22h, which is the peak of DNA synthesis. The increases in cyclic AMP were prevented by beta-adrenergic blocking agents, propranolol and pindolol, without affecting ornithine decarboxylase induction or DNA synthesis. The alpha-blocking agents, phenoxybenzamine and phentolamine, given at the time of partial hepatectomy, delayed the rise in ornithine decarboxylase normally found 4h after operation, but did not affect DNA synthesis. If the alpha-blocking agents were given at 9-12h or 18h, the onset of DNA synthesis was delayed. Phenoxybenzamine did not affect the induction of ornithine decarboxylase in intact rat livers by glucagon or growth hormone, but did inhibit induction by dexamethasone. The induction of ornithine decarboxylase produced by dexamethasone was inhibited by 17alpha-hydroxy-progesterone; this compound also blocked the induction of ornithine decarboxylase in livers of partially hepatectomized rats.


The relative stability of liver cytosol enzymes incubated in vitro. 1. Relative rates of enzyme inactivation were measured in liver slices, homogenates and cytosol fractions as well as in the presence of trypsin and at acid pH. The enzymes chosen are all present in the cytosol fraction of rat liver, and have widely different degradation rate constants in vivo. 2. The inactivation rates of lactate dehydrogenase, fructose bisphosphate aldolase, glucose 6-phosphate dehydrogenase, glucokinase, phosphoenolpyruvate carboxykinase (GTP), l-serine dehydratase and thymidine kinase in liver preparations at neutral pH are in a similar order to the rate constants of degradation of these enzymes in the intact animal. 3. The two exceptions of this general correlation were tyrosine aminotransferase, which was stable in vitro but not in vivo, and glyceraldehyde phosphate dehydrogenase, which shows the reverse pattern. 4. These findings generally support the concept that the same factors are responsible for enzyme inactivation in vitro as occur in the intact tissue.


Pyruvate dehydrogenase activity in the normal and cryptorchid rat testis.


Veterans Administration conference of prosthetics and sensory aids. July 20-23, 1975. Preface.


Veterans Administration conference of prosthetics and sensory aids. July 20-23, 1974. Editorial: A basic theme of cooperation.


The role of catecholamines and nerves in ovulation.


The effect of ovulation on the response of the rabbit oviduct to adrenergic agonists in vitro.


Upper-limb prosthetics workshop.

Upper-limb prosthetics workshop.


Lower-limb prosthetics workshop.

Lower-limb prosthetics workshop.


Orthotics workshop.

Orthotics workshop.


Sensory-aids workshop.

Sensory-aids workshop.


The spinal-cord-injured patient workshop.


The effect of a beta-adrenergic agent on prostaglandin stimulated labor. This study examines the effect of beta-adrenergic administration (orciprenaline) on oxytocin- and prostaglandin-induced uterine activity at term in 10 patients in whom labor was induced.  An amniocentesis was performed and Pethidine 50 mg was administered intravenously.  Labor was induced by oxytocin or prostaglandin F2alpha (PGF2alpha) using a constant-rate infusion pump.  When uterine activity was compatible with early labor, 0.25 mg of orciprenaline was administered.  When the uterine activity returned to the original level, the 1st drug was discontinued and the other substituted.  When the membranes were ruptured, labor was allowed to continue.  All cases were delivered vaginally within 18 hours.  Results show that:  1) there is no difference in the effect of orciprenaline on the cardiovascular variables, pulse rate, and blood pressure when labor was induced with PGF2 alpha and oxytocin;  2) maternal tachycardia and an increase in pulse pressure were noted in all cases; 3) there was no alpha adrenergic effect of the PG at the dosage used; and 4) uterine activity was abolished for a longer period when the contractions were induced by oxytocin in spite of an initial higher level of uterine activity in the PG series.  Orciprenaline, a resorcyl ethanolamine and beta-adrenergic stimulant, was less effective in decreasing uterine activity stimulated by PGF2alpha than by oxytocin.  These findings conform with the hypothesis that PGs may be implicated in the stage of rapid cervical dilation, as beta-adrenergics are known to be less effective in this stage of premature labor.  A new approach is suggested in that antiprostaglandins may be effective at a later stage in premature labor.


World population year, 1974.

World population year, 1974. The first intergovernmental World Population Conference was held in Bucharest in August 1974.  Social and economic development were given importance in the revised plan as well as reduction of morbidity and mortality, reduction of infant and child mortality. infertility, illegal abortion, disease and malnutrition, the right of persons  to decide on the number and spacing of their children, increasing health manpower and facilities, participation of women in the social and political life of a country.  Recommendations for further research were made.  It is also recommended that every country take a population census between 1975 and 1985.


Studies on a cytochrome oxidase antibody. IV. An immunologic impurity.