Temperature-sensitive mutants of murine leukemia virus. IV. Further physiological characterization and evidence for genetic recombination. Several temperature-sensitive mutants of the Rauscher strain of murine leukemia virus representing three distinct physiological groups have been further characterized. Genetic recombination between different pairs of these mutants has been demonstrated. Several representative genetic recombinants were isolated and shown to replicate equally well at the permissive (31 C) and nonpermissive (38 C) temperatures and to have serological characteristics of the wild-type parental virus. Alternative models for the mechanisms involved in recombination between type C RNA viruses are discussed.


Differential renal protein clearance in toxaemia of pregnancy.


Differential susceptibility of mouse trophoblast and embryonic tissue to immune cell lysis.


Properties and reaction mechanism of DT diaphorase from rat liver.


Properties of histocompatibility (HL-A) determinants. 3. The independent preciptation and epitope valence of soluble HL-A determinants.


Properties of an antigenic polysaccharide from Corynebacterium parvum. Corynebacterium parvum strain 10390 is an antitumor agent and stimulant of the reticuloendothelial system and produces a soluble antigen towards the end of its growth cycle. This material, which is a cell wall component and can also be released from the organism by acid or alkaline hydrolysis, has been purified. It is an acidic polysaccharide of molecular weight 100,000 to 150,000 and contains galactose, glucose, fucose, N-acetylgalactosamine, N-acetylglucosamine, uronic acids, sialic acids, and a small proportion of amino acids. The antigen gives a precipitin reaction with antisera raised against the whole organism and also binds to animal cells. The antigenic determinants are extremely resistant to oxidation, reduction, and enzymatic and chemical hydrolysis, but the single cell-binding site is destroyed by alkali and also by Helix pomatia digestive juice, alginase, and neuraminidase without substantially affecting the molecular weight. This site is inaccessible until the molecule is released from the cell surface. The possibility that the soluble antigen is the biologically active fraction of C. parvum is discussed.


Properties of intracytoplasmic A particles isolated from oncornavirus-producing human cells. A particles with the diameter of 70 to 80 nm were isolated from the cytoplasm of HEp-2, HeLa, and AO cells producing oncornavirus of Mason-Pfizer-like type. Most of the A particles banded at 1.23 to 1.24 g/ml, whereas 3 to 10% banded at 1.29 g/ml in equilibrium sucrose gradients. They banded at 1.30 g/ml in CsCl gradients suggesting that they contained 8% RNA. Individual A particles sedimented at 200 to 250S in velocity sucrose gradients, but their significant part was found aggregated and sedimented at more than 300S. They were resistant to RNase digestion. A particles possessed polymerase activity which was preferentially activated by Mn(2+) rather than by Mg(2+), the RNA template being 60S RNA. Cross-hybridization with two DNA products and immunoassay showed that A particles and Mason-Pfizer-like oncornavirus produced by the same cells contained neither homological RNA sequences nor common antigens, suggesting that A particles are not intracellular precursors of Mason-Pfizer-like oncornavirus but represent an independent oncornavirus. Hybridization of A particle RNA with excess of cellular DNA revealed about 20 proviral copies per HEp-2 cell genome and no proviral copies in human embryo and placenta cell genomes.


Properties of ribosomes from Streptomyces erythreus and Streptomyces griseus. Ribosomes from an erythromycin-producing strain, Streptomyces erythreus, lacked affinity for erythromycin and were also resistant to other macrolide antibiotics (leucomycin, spiramycin, and tylosin) and to lincomycin, whereas Streptomyces griseus B(3) ribosomes were susceptible to all of these antibiotics.


Repression of enzymes of nitrogen catabolism by methylammonium and caesium chloride in strains of Aspergillus nidulans insensitive to ammonium repression.


Expression of selected antigens on the surface of cultured neural cells.


Diabetes mellitus death-rates in England and Wales 1920-70 and food supplies.


Enuretic alarm trainer for night and day.


Secretion of dihydrotestosterone by human prostate in benign prostatic hypertrophy.


Excretion of penicillin G and dihydrostreptomycin from quarters of cows with experimentally induced staphylococcal mastitis.


Society's misfits. The homeless alcoholic.


The evidence for involvement of type C RNA tumor viruses in human acute leukemia.


The fate of patients after incomplete resection of bronchial carcinoma.


Key features for the identification of 250 hospital isolates of Pseudomonas aeruginosa, with observation on their in vitro susceptibility to 10 antibiotics.


5. Effects on staining of the presence of various kinds of dye impurities.


Purification of avian myeloblastosis virus DNA polymerase by affinity chromatography on polycytidylate-agarose. Polycytidylic acid [poly(rC)] covalently linked to cyanogen bromide-activated agarose is an effective affinity matrix for the RNA-dependent DNA polymerase from avian myeloblastosis virus. Poly(rC)-agarose is capable of binding large quantities of avian myeloblastosis DNA polymerase, which is then eluted by using a linear KCl gradient of increasing concentration. The DNA polymerase isolated from crude, detergent-disrupted virions by a single pass through columns of poly(rC)-agarose appears nearly homogeneous (approximately 90% pure) as determined by sodium dodecyl sulfate-polyacrylamide disc gel electrophoresis. Complete recovery of input enzymatic activity was obtained. Results suggest that polyribonucleotide columns may provide a high-yield, rapid method for the purification of oncornaviral DNA polymerase.


The eye and its disorders. 28. The retina.


The eye and its disorders. 18. Squint.

The eye and its disorders. 18. Squint.


Management of patients with primary immune deficiency syndromes.


Antigen-initiated B lymphocyte differentiation. Kinetics and T lymphocyte dependence of the primary and secondary in vitro immune responses to the hapten 4-hydroxy-3-iodo-5-nitrophenylacetic acid presented on the carrier polymerised bacterial flagellin.


Polygenic factors controlling drug response.


Cytogenetic and linkage studies of a familial 15pplus variant.


Antigenic relationships among strains of African swine fecre virus.


Autogenous immunity to endogenous RNA tumor virus: differential reactivities of immunoglobulins M and G to virus envelope antigens. The autogenous humoral immune response of mice to their endogenous leukemia virus has been examined in terms of the reactivities of individual classes of antibody present in normal B6C3F(1) serum. Whole serum and the immunoglobulin (Ig) M and IgG fractions of serum from animals of different age groups were compared by radioimmune precipitation assays and viral infectivity neutralization assays. Both IgM and IgG fractions were able to precipitate virus, although not as effectively as whole serum. Virus-specific antibody levels, as well as total antibody concentrations in whole serum, appeared to increase with age. Sodium dodecyl sulfate gel electrophoresis analysis was performed with immune precipitates obtained when whole serum or 19 or 7S fractions from animals of different age groups were reacted with disrupted virus. The 19S antibody fraction reacted with three antigenic determinants on the viral envelope. These antigens have apparent molecular weights of 17,000, 43,000, and 68,000. The last two appear to be glycoproteins and may correspond to the M(2) and M(1) antigens. In contrast, the 7S component reacted only with the 17,000-molecular-weight protein. Neutralization assays against BALB:virus-2, a xenotropic endogenous mouse type C virus, revealed that 19S and whole serum but not the 7S fraction possessed neutralizing activity. These findings indicate that there are differential reactivities of IgM and IgG antibodies in normal serum of B6C3F(1) mice, with respect to both recognition of viral envelope antigens and neutralization of endogenous MuLV. These results are consistent with the hypothesis that the autogenous humoral immune response is a systemic host function that may be important in the regulation of endogenous type C virus expression in vivo.


Antigens of murine leukemia viruses.

Antigens of murine leukemia viruses.


Ontogeny of B-lymphocyte function. I. Restricted heterogeneity of the antibody response of B lymphocytes from neonatal and fetal mice. The ontogeny of the ability of B lymphocytes to produce an antihapten response which is heterogeneous with respect to affinity for the antigenic determinant was studied in a cell transfer system. The heterogeneity of affinity of the immune response of lethally irradiated mice reconstituted with syngeneic, adult thymus cells and fetal or neonatal tissues as a source of B lymphocytes was studied. It was found that B cells from 17 day fetal liver or neonatal liver are highly restricted with respect to heterogeneity of affinity as compared with adult spleen or bone marrow. The B-cell population achieves an adult character with respect to heterogeneity of affinity by 2 wk of age. The peripheral lymphoid tissues (spleen) appear to mature in this respect more rapidly than do central lymphoid tissues (bone marrow). Spleens from 10-day old donors behave in an adult, heterogeneous manner while bone marrow from the same donors exhibit a marked restriction in heterogeneity of affinity. Germfree mice produce an immune response which is indistinguishable from conventionally reared adult animals with respect to heterogeneity of affinity. The earlier appearance of the ability to transfer a heterogeneous immune response in spleen as compared with bone marrow suggests that the increasing heterogeneity of the B-lymphocyte population which occurs between birth and 2 wk of age is the result of a differentiation event and not of a somatic mutation or recombination event.


I region-associated antigen system (Ia) of the mouse H-2 gene complex. Further definition of Ia secificities with restricted anti-Ia antisera.


Na+-gradient-stimulated AIB transport in membrane vesicles from Ehrlich ascites cells.


Sjögren's syndrome in systemic lupus erythematosus. Clinical and subclinical manifestations.


Integration of measles virus nucleic acid into the cell genome.


On the mechanism of action of glycoprotein from Candida albicans.


The hemagglutinating and neuraminidase protein of a paramyxovirus: interaction with neuraminic acid in affinity chromatography.


On the relationship between the frequency of association and the nucleolar constriction of individual acrocentric chromosomes.


Biochemical suppression of tumor virus activity.


Further studies on antigenic regions of bovine encephalitogenic protein with the lymph node cell transformation test in rabbits.


Synthesis of viral-specific polypeptides in Mengo virus-infected L cells: evidence for asymmetric translation of the viral genome.


Synthetic RNA-dependent DNA polymerase from calf thymus.


Two high affinity estrogen binding proteins of different specificity in the immature rat uterus cytosol.


Alcoholic brain damage: anticoagulant therapy.


Morphologic observations on four cases of SED congenita.


Morphological and functional maturation of human thymic epithelium in culture.


Morphological contributions to monoamine pharmacology.


Psychological death: an adaptive response to life-threatening illness.


Synchronized combination bleomycin-methotrexat in regional chemotherapy of orofacial carcinomas.


Prophylactic use of intravenous saline in patients with chronic renal failure undergoing major surgery.


Plethysmographical and clinical observations on Bricanyl therapy in Raynaud's phenomenon.


Prophylaxis of viral hepatitis.

Prophylaxis of viral hepatitis.


Carriage of Staphylococcus aureus and beta haemolytic streptococci in relation to race.


Palliative cryosurgical treatment of the inoperable rectum carcinoma.


Palliative intubation in the management of esophageal carcinoma.


Possible detection of presensitization to donor alloantigens by immunofluorescence.


Clinical evaluation of tumor scanning with 57Co-bleomycin.


Atypical lymphocytes in acute infectious mononucleosis. Identification by multiple T and B lymphocyte markers.


Clinico-pathologic conference.

Clinico-pathologic conference.


Clinical significance of hematospermia.

Clinical significance of hematospermia.


Replication of oncornavirus-like particle in human breast carcinoma cell line, MCF-7.


Leslie Wakefield Hale.

Leslie Wakefield Hale.


Studies on experimental itch induced by kallikrein and bradykinin.


Studies on guinea pig skin cell cultures. 3. Minimum cell numbers for establishment.


Studies of excitable membranes. I. Macromolecular specializations of the neuromuscular junction and the nonjunctional sarcolemma. The neuromuscular junctions and nonjunctional sarcolemmas of mammalian skeletal muscle fibers were studied by conventional thin-section electron microscopy and freeze-fracture techniques. A modified acetylcholinesterase staining procedure that is compatible with light microscopy, conventional thin-section electron microscopy, and freeze-fracture techniques is described. Freeze-fracture replicas were utilized to visualize the internal macromolecular architecture of the nerve terminal membrane, the chemically excitable neuromuscular junction postsynaptic folds, and the electrically excitable nonjunctional sarcolemma. The nerve terminal membrane is characterized by two parallel rows of 100-110-A particles which may be associated with synpatic vesicle fusion and release. On the postsynpatic folds, irregular rows of densely packed 110-140-A particles were observed and evidence is assembled which indicates that these large transmembrane macromolecules may represent the morphological correlate for functional acetylcholine receptor activity in mammalian motor endplates. Differences in the size and distribution of particles in mammalian as compared with amphibian and fish postsynaptic junctional membranes are correlated with current biochemical and electron micrograph autoradiographic data. Orthogonal arrays of 60-A particles were observed in the split postsynaptic sarcolemmas of many diaphragm myofibers. On the basis of differences in the number and distribution of these "square" arrays within the sarcolemmas, two classes of fibers were identified in the diaphragm. Subsequent confirmation of the fiber types as fast- and slow-twitch fibers (Ellisman et al. 1974. J. Cell Biol.63[2, Pt. 2]:93 a. [Abstr.]) may indicate a possible role for the square arrays in the electrogenic mechanism. Experiments in progress involving specific labeling techniques are expected to permit positive identification of many of these intriguing transmembrane macromolecules.


Studies on human antiglobulins. Some properties of a "new" type.


Studies on mitochondrial structure and function in Physarum polycephalum. III. Electron microscopy of a large amount of DNA releases from a central body in mitochondria by trypsin digestion.


Studies on the determinant group of the type specific antigen of Leptospira kremastos strain Kyoto.


Studies on the depletion and accumulation of microvilli and changes in the tubulovesicular compartment of mouse parietal cells in relation to gastric acid secretion. Gastric parietal cells in mice present a spectrum of microscopic appearances due mainly to variations in the abundance of the tubular and vesicular component of the cytoplasm and in the size and number of microvilli lining the intracellular canaliculi. Differences in the range of forms among parietal cells of fasting versus fed mice were not especially striking, but cells with very numerous tubules and vesicles were more common after fasting. However, in mice treated with drugs or hormones that induce acid secretion, parietal cells were more uniform in appearance. There was a marked reduction of these cytoplasmic membranes and a concomitant increase in both the number and size of microvilli. Measurements of acid secretion in control animals and in animals treated with acid secretagogues indicated hydrogen ion secretion contemporaneous with depletion of the cytoplasmic tubulovesicular membranes and with increase of the microvilli. In mice with inhibited acid secretion, parietal cells showed an accumulation of cytoplasmic tubules and vesicles and reduction in the numbers of microvilli. Stereological methods were used to quantitate 10 different parietal cell compartments. Tracer studies with lanthanum did not reveal continuity between the tubules and the plasma membrane. However, there were regions of close apposition between the tubulovesicular membranes and the cell membrane of the canaliculus, and instances where cytoplasmic tubules extended from the cell into the core of enlarged microvilli.


Studies on the effects of basic proteins on myelination in nerve tissue cultures.


Studies on the role of calcium in the anaphylactic histamine release from isolated rat mast cells.


Studies on the motility of Plasmodium sporozoites.


Studies on the storage polysaccharides of the Coccidia Eimeria bovis and Eimeria stiedai.


Specificity and cross-reactivity of antisynaptosome antibodies.


Studies with lectins on the surface carbohydrate structures of mycoplasma membranes. The surface carbohydrate structures on the cell membranes of various mycoplasma species have been investigated by using lectins, which are sugar-specific proteins. Carbohydrate structures presumably bound to glycolipids, with both galactose and glucose units, were found to be exposed on the surface of Mycoplasma pneumoniae and its temperature-sensitive mutants, M. mycoides var. mycoides and capri, M. pulmonis, M. gallinarum, and M. gallisepticum. Lipid-bound glucose was found on M. neurolyticum. The possible relationship of the lipid-bound surface carbohydrate groups to the known serological cross-reactions and lipid compositions of the various mycoplasma species is discussed. Intact Acholeplasma laidlawii and M. fermentans have no lectin-binding sites exposed on their surfaces; galactose groups were discovered only after Pronase digestion of the organisms, suggesting that their glycolipids are hidden under a protein layer. Neither intact nor Pronase-digested M. hominis reacted with the lectins; this is fully consistent with the lipid composition of this organism, which contains glycolipids. The lectins from Vicia cracca and Phaseolus vulgaris, which react with N-acetyl-galactosamine groups, agglutinated M. gallinarum, M. gallisepticum, M. mycoides var. capri, and M. pulmonis. The agglutinability was lost after Pronase treatment, indicating that the corresponding carbohydrates are presumably protein bound. They may be correlated with the extracellular structures observed by electron microscopy of both sectioned and negatively stained mycoplasma species.


Preliminary experience in the treatment of cardiac arrhythmias with aprindine.


The immunohistopathology of glomerular antigens. The glomerular basement membrane, collagen, and actomyosin antigens in normal and diseased kidneys. The immunofluorescent localization of antisera to human glomerular basement membrane (GBM), collagen, and smooth muscle actomyosin was examined in 15 specimens of normal renal tissue and 98 specimens from patients with renal disease. The anti-GBM and anticollagen antisera normally localize to GBM, while antiactomyosin localizes to the mesangium. Diabetic nephropathy revealed a striking expansion of mesangial material reacting with antiactomyosin. In contrast, the expanded mesangium in membranoproliferative glomerulonephritis did not react with antiactomyosin, and the GBM localization of anti-GBM and anticollagen sera was similarly lost. The thickened GBM in diabetes mellitus and membranous nephropathy reacted with anti-GBM and anticollagen, but with accentuation of staining on the inner aspect of the GBM. In proliferative glomerulonephritis there was a moderate increase in the distribution of actomyosin. Glomerular sclerosis and hyalinization in all diseases studied was accompanied by a loss of immunofluorescent staining for all glomerular antigens, including collagen.


Combined treatment of malignant tumor with irradiation and bleomycin.


Uridine monophosphate kinase: A new genetic polymorphism with possible clinical implications.


The influence of paraproteinaemia on the technicon automated platelet counter. In five patients whose serum contained a paraprotein it has been shown that platelet counts obtained using the Technicon AutoCounter are much too high. In two patients with IgG gammopathy the error is caused by some factor in the red cells lysed by the diluent solution 2M urea. In three patients with IgM gammopathy spurious platelet counts were produced by platelet-poor plasma when diluted with 2M urea. It is recommended that at the present time no patient known to have a paraprotein should have platelet counts performed on the AutoCounter.


Smoking and the heart. The basis for action.


Binding of secretory component to dimers of immunoglobulin A in vitro. A model for the interactions of the two moieties in secretory immunoglobulin A.


Binding of rifampicin by human plasma proteins.


New inhibitor of reagin-mediated anaphylaxis.


Binding of soluble immune complexes to human lymphoblastoid cells. II. Use of Raji cells to detect circulating immune complexes in animal and human sera. Cells from a human lymphoblastoid cell line (Raji), with B-cell characteristics, and having receptors for human IgG Fc, C3b, and C3d, were used in an immunofluorescence test as in vitro detectors of immune complexes in animal and human sera. By this test, as little as 200-300 ng aggregated human gamma globulin or immune complexes per ml serum could be detected. The receptors for IgG Fc on the Raji cells were shown to be inefficient in detecting aggregated human gamma globulin and binding of aggregates to these receptors was inhibited by physiologic concentrations of 7S human IgG. Enhancement of aggregated human gamma globulin binding and binding of immune complexes formed in vitro to Raji cells was observed when the receptors for complement on these cells were used. By using the receptors for complement on Raji cells, circulating immune complexes were detected in rabbits with acute serum sickness, in mice with acute lymphocytic choriomeningitis virus infection, and in humans with immune complex type glomerulonephritis. The Raji cell test may be useful in detecting complement fixing immune complexes in different disease states, in monitoring circulating complexes in patients with immune complex diseases and in identifying the antigen(s) responsible for the induction of pathogenic immune complexes in humans and animals.


Carcinoma of the corpus and cervix--some thoughts about diagnosis and treatment.


EEG instrumentation standards: report of the Committee on EEG Instrumentation Standards of the International Federation of Societies for Electroencephalography and Clinical Neurophysiology.


The influence of portosystemic shunt operation on immunoglobulins and Escherichia coli antibodies in patients with cirrhosis of the liver.


The interaction of cationic liposomes containing entrapped horseradish peroxidase with cells in culture. Cationic liposomes composed of sphingomyelin, cholesterol, and stearylamine were prepared with horseradish peroxidase trapped inside. Stable particles were formed in which 10-12% of the enzymic activity appeared to be located at, or near, the outer surface of the liposome. Adsorption and uptake of liposomes by HeLa cells were followed cytochemically by electron microscopy and quantitated by enzyme assay and by the distribution and fate of particles labeled with [(14)C]cholesterol and [(125)I]horseradish peroxidase. The particles were adsorbed by HeLa cells at least 300 times as efficiently as was free horseradish peroxidase. Many of the particles remained at the cell surface, but numerous membrane-bound cytoplasmic inclusions were observed to contain peroxidase-staining material. In addition, many areas of the cell membrane gave a positive staining reaction. It was concluded that many particles (presumably the larger ones) did not gain access to the interior of the cells, many were phagocytized, and some enzyme was transferred to the cell membrane, perhaps as a result of fusion of the liposomal membrane with the cell membrane.


Continuous ECG (Holter) recordings in the evaluation of antiarrhythmic drugs.


Cardiovascular effects of halothane in the stump-tailed macaque during spontaneous and controlled ventilation.


Impairment by cholestyramine of dicumarol and tromexan absorption in rats: a potential drug interaction.


Precipitating antibodies in anti-erythropoietin sera.


The isolation of kidney brush border.

The isolation of kidney brush border.


Conditions for optimal MTV synthesis in vitro and the effect of steroid hormones on virus production.


In vitro cytotoxicity of human lymphocytes: reduction of the lymphocyte cytotoxicity induced by antibodies or phytohemagglutinin by removing immune-complex-binding cells.


Committee on Educational Affairs of ASBC.


Calcium balance in pregnancy.

Calcium balance in pregnancy.


Viral hepatitis--the state of the art.

Viral hepatitis--the state of the art.


Passive transport of the stria vascularis.


Cyril Wyllie Kesson.

Cyril Wyllie Kesson.


Fetal malformations and abnormalities.

Fetal malformations and abnormalities.


Vinblastine-induced blockage of orthograde and retrograde axonal transport of protein in retinal ganglion cells.


Simulataneous occurrence of Neisseria gonorrhoeae, Candida albicans, and Trichomonas vaginalis.


Shoulder-hand syndrome.

Shoulder-hand syndrome.


Muscle fibrillation induced by blockage of axoplasmic transport in motor nerves.


Isoelectrofocusing of human plasma lipoproteins in polyacrylamide gels: diagnosis of type III hyperlipoproteinemia ("broad beta" disease).


An electron microscopic investigation of the surface coat of visceral yolk sac endoderm cells in the guinea pig.


An electrophoretic method for detection of unbound bilirubin and reserve bilirubin binding capacity in serum of newborns.


Peculiar secretory IgA system identified in chickens.


Acrylic cement and the cardiovascular system.


Complement components and IgE in bronchial asthma.


Nodular cutaneous reactive histiocytosis caused by an unidentified microorganism: report of a case.


Facility design and equipment considerations for the team practice of dentistry.


The liver in kwashiorkor. A clinical and electron microscopical study.


Cytologic diagnosis in allergic disorders.


Serological responses of multiple-sclerosis patients and controls to a virus isolated from a multiple-sclerosis case.


Cytology of rheumatoid synovial cells in culture. III. Significance of isolates of epithelial cell lines.


Development of immunotherapy for infections due to Pseudomonas aeruginosa.


Is closed circuit TV a worth-while teaching aid?


Development of contact sensitivity to DNFB in guinea pigs genetically differing in their response to DNP skin protein conjugates.


Results of studies using an on-line automatic analyzer of sleep stages in the rat.


Triglyceride-lowering effect of chenodeoxycholic acid in patients with endogenous hypertriglyceridaemia.


Serum insulin response to glucose infusion in hyperthyroid dogs.


Serum protein pattern in Addis Ababa preschool children.


Simultaneous measurement of bromsulphalein initial distribution volume and 131I-albumin plasma volume in patients with liver disease. A study of 195 patients and a review of the literature.


Serum proteins: diagnostic significance of electrophoretic patterns.


Plasma kinins and the jarisch-herxheimer reaction.


Plasma testosterone levels and acne.

Plasma testosterone levels and acne.


Traumatic encephalopathy in a young boxer.


Automatic suppression of eye movement and muscle artifacts when averaging tape recorded cerebral evoked potentials.


Estimation of chromatin patterns at G1, S, and G2 of the cell cycle.


The medical management of hyperthyroidism.


The mentally retarded are not different.


A membrane filter method for correlating colposcopic, histological, and cytological pictures: the application to nuclear DNA measurements. A method is described for collecting cells on membrane filters in their natural topographical relationships, so that they can be correlated with exact areas in the cervix and with punch biopsy specimens. The same cell sample, after study with Papanicolaou stain, can then be used for other methods without the cells losing their spatial relationships. An example is given of an early cervical carcinoma in which, using a photographic method with a simple type of photometric device, the nuclear DNA was measured from accurately identified normal and neoplastic areas.


The metabolism of IgG(T) in the newborn foal.


Endemic cretinism of recent onset in New Guinea.


An immunoelectron microscopy study of Soehner-Dmochowski murine sarcoma virus following passage in rats and hamsters.


Dynamics of axoplasmic transport in the optic system of the rat.


A comparative immunochemical study of the serum proteins of several Galapagos iguanids.


A comparison of techniques for determining prenatal sex from liquor amnii. The cellular content of amniotic fluid is primarily composed of desquamated squamous cells. Depending on their site of origin and maturation, the staining characteristics of the nuclei and cytoplasm differ. Prenatal foetal sex was determined by comparing the proportion of anucleate pyknotic nuclei to vesicular nuclei using Papanicolaou's stain and the proportion of red cells to blue cells using Shorr's stain.Both techniques allow an accurate assessment of prenatal sex to be made after 30 week's gestation. There is a close correlation between the two methods.


A simple method for the preparation of antiserum to bovine immunoglobulins using immunosorbents.


Attempts at morphological identification of the hemopoietic stem cell in rodents and primates.


Attempts to measure plasmalemma and tonoplast electropotentials in small cells of the moss Mnium using centrifugation techniques.


Zenon Buczowski.

Zenon Buczowski.


Rat myelin proteins. Compositional changes in various regions of the nervous system during ontogenetic development.


Diurnal changes in mitotic activity and DNA synthesis in normal and tumor tissues of rats.


B. Anathaswami Rao.

B. Anathaswami Rao.


An analysis of some discriminative properties of d-amphetamine.


The nature of Reed-Sternberg cells and other malignant "reticulum" cells.


Infancy and childhood.

Infancy and childhood.


Iodine contamination in iodometric measurements of thyroid hormones.


Canine lipoproteins and atherosclerosis. I. Isolation and characterization of plasma lipoproteins from control dogs.


Immune deposits in passive Arthus phenomenon. Electron microscopic demonstration by use of peroxidase-labeled antibody.


Canine lipoproteins and atherosclerosis. II. Characterization of the plasma lipoproteins associated with atherogenic and nonatherogenic hyperlipidemia.


Immune responses against native and chemically modified albumins in mice. II. Effect of electric charge and conformation on the humoral antibody response and on helper T cell responses.


Iodine-induced toxic diffuse goitre.

Iodine-induced toxic diffuse goitre.


Cutaneous uses of the antiproliferative drugs.


Strong stimulation of CBA lymphocytes in the mixed lymphocyte interaction with cells from the H-2-indentical strain C3H.


An inhibitor of DNA polymerase which appears after inoculation of BALB-c mice with syngeneic tumors.


Chronic septal hepatitis.

Chronic septal hepatitis.


Chronic sinusitis in children. Comments on pathogenesis and management.


Scanning immunoelectron microscopy of mouse B and T lymphocytes.


Immunization against Pseudomonas in infection after thermal injury.


Immunobiology of colon carcinomas.

Immunobiology of colon carcinomas.


Immunological demonstration of alpha-fetoprotein in uterine cytosol from immature rats. An immunological demonstration of alpha-fetoprotein (AFP) in uterine cytosol from immature rats is reported.  The identity between serum AFP and the estrogen binding 4.5 S macromolecular complex of uterine cytosol was demonstrated by the use of a specific immunoadsorbent to AFP.  Analysis of the sedimentation profile in glycerol gradients of uterine cytosol incubated with tritiated estrone or estradiol suggests that the total estrogen binding capacity of the 4.5 S complex is provided by AFP.  Changes of AFP content in rat uterus with the age of the animals suggest that this protein is probably present in the cytosol as a serum contaminant.


Immunological distinction between the possible origins of enzymatic activity in a polypeptide fragment of staphylococcal nuclease.


Eosinophilic argentaffin cells in the human appendix.


Diagnostic electron microscopy of faeces. I. The viral flora of the faeces as seen by electron microscopy. A method is described for examining viruses in faeces by direct electron microscopy using negative staining. The particles found in a group of patients with gastroenteritis and a group with other conditions are compared. Small particles in the range of sizes covering parvoviruses and enteroviruses were found about as frequently in each group. Many of these were probably bacteriophages. Many bacteriophages with tails of various sizes and lengths were found. Adenoviruses were found in five of eight patients from whom they were isolated. Reovirus- (or orbivirus-) like particles were found associated with some gastroenteritis patients.


Prognostic value of estrogen receptor to endocrine ablation in cancer of the breast.


Immunosuppression with ethidium bromide: effect on lymphocyte blastogenesis and antibody plaque forming spleen cells.


Demonstration of geniculocortical and callosal projection neurons in the squirrel monkey by means of retrograde axonal transport of horseradish peroxidase.


Client education. Technics in small animal practice.


Ribonucleic acid. I. The test-tube synthesis of a viral nucleic acid. II. The development and use of molecular hybridization.


A convenient stimulus and situation coding system for the tape recording of event-related potentials.


Radioautographic studies on the intracellular distribution of bleomycin-14C in mouse tumor cells.


Histochemical studies of mucus-secreting cells in the gut of a coral fish, Chelmon rostratus Cuvier.


Histochemical study on neural crest cells in mice embryos.


Dissociation of lymphocyte activating determinants from recognition sites in mouse MLR.


Leucocyte histamine release studies and skin tests with aggregated gamma-globulin in hypogammaglobulinaemia.


Oligodendroglial ensheathment of axons during myelination in the developing rat central nervous system. A serial section electron microscopical sttudy.


Carbocyanine dye metachromasia of sialidase-sensitive polyanions in sera from normal and tumor-bearing mice.


Reproductive immunology.

Reproductive immunology.


Use of selective, intrahepatic, portal venography and in vivo coloration in planning segmental hepatic resection.


Use of DNA-DNA annealing to detect new virus-specific DNA sequences in chicken embryo fibroblasts after infection by avian sarcoma virus. Labeled, virus-specific DNA synthesized in vitro by the virion-associated polymerase of avian sarcoma virus (ASV) was used to measure virus-specific sequences in cell DNA in three ways: (i) by determining the effect of cell DNA upon the reassociation rate of double-stranded polymerase products; (ii) by measuring the kinetics of annealing of single-stranded polymerase product (cDNA) to cell DNA; or (iii) by measuring the amount of cDNA which anneals to a large excess of cell DNA. With these three assays and modifications of them, we show that fewer than five copies of ASV-specific DNA sequences are present per diploid cell in uninfected chicken embryos; that a two- to several-fold increase in copy number of viral DNA follows infection by ASV; that infection introduces to the cell viral sequences not present before infection; and that DNAs from uninfected Pekin duck and Japanese quail embryos show no homology with DNA synthesized by the ASV polymerase. Some of these results differ from data in a previous report from this laboratory (H. E. Varmus, R. A. Weiss, R. R. Friis, W. Levinson, and J. M. Bishop, 1972) and, in general, reconcile our observations with those from other laboratories.


Retrograde thalamic degeneration: observations using a modification of the Fink-Heimer silver impregnation technique.


Angiographic management of postoperative bleeding.


Amyloid in normal and pathological parathyroid glands. Two groups of parathyroid glands have been examined for intrafollicular amyloid. In glands removed surgically it was found in nine cases (16%); in glands removed at necropsy it was found in 23 cases (46%). Also, two postmortem cases of systemic amyloidosis showed involvement of parathyroid glands with an entirely different distribution of amyloid. Compared with other amyloids the tinctorial properties of the intrafollicular amyloid were uniform in reactions with Congo red and Thioflavin T, but the intensity of the staining reaction for amino acids tryptophan and tyrosine was not constant for the follicle amyloid. This variation may be a feature of a maturation process but the derivation of this amyloid remains uncertain.


Thyroid physiology and functional tests.


Radioimmunoassay of alpha fetoprotein in the diagnosis of malignancy.


Thyroid-stimulating hormone binding to beef thyroid membranes. Relation to adenylate cyclase activity.


Radioimmunoassay for the major structural protein of hamster type C viruses. A radioimmunoassay for the major, group-specific antigen (p30) of hamster type C viruses was developed. The test detected approximately 5 ng of viral protein per ml and was highly specific for hamster viruses when used with homologous antibody. Comparison of three hamster viruses, two being mouse-hamster pseudotypes, in homologous and heterologous intraspecies assays, showed no evidence of type specificity for these proteins. The pseudotype viruses showed no evidence of mouse virus p30 antigenic determinants. An interspecies antigen assay employing (125)I-labeled hamster p30 and anti-feline p30 was completely inhibited by cat (feline leukemia virus), hamster, and rat viruses, to a slightly lesser degree by mouse viruses, and only poorly by RD 114 and Gibbon ape viruses. The Mason-Pfizer virus did not inhibit this assay. Hamster p30 was detected by radioimmunoassay in individual embryos from two LSH hamsters and in several adult tissues, excluding muscle at levels below that required for detection in complement-fixation tests.


A cholinergic mechanism in the spinal cord of cats.


Histological findings in parakeratosis pustulosa.


Anatomopathologic basis for the cryosurgery of the prostate.


Regional monitoring of ambient air carbon monoxide in Fairbanks, Alaska.


Proton evolution associated with the photooxidation of water in photosynthesis.


Anthony Richard May.

Anthony Richard May.


Macrophage-lymphocyte clusters in the immune response to soluble protein antigen in vitro. We have studied the physical interaction between macrophages and lymphocytes during the immune response to purified protein derivative of tuberculin (PPD) in vitro. Mixtures of peritoneal macrophages and lymph node lymphocytes from guinea pigs immunized with tubercle bacilli formed cell clusters during 20 h of culture with PPD. The number of clusters produced was correlated to the number of immune lymphocytes in the cultures. Peritoneal macrophages which had been pulsed with PPD and untreated lymph node lymphocytes produced cell clusters in the absence of free PPD in numbers equivalent to those produced by the same cells in the presence of free PPD. In cultures containing a mixture of PPD-pulsed macrophages, not-pulsed macrophages, and immune lymphocytes with no free PPD, cell clusters developed mainly between the antigen-pulsed macrophages and lymphocytes. Cluster formation was antigen-specific with the specificity residing in the lymphocytes, mainly or exclusively in the T lymphocytes. These data indicate that in the process of cell cluster formation macrophages serve as antigen-binding (or -processing) cells, while a subpopulation of lymphocytes interact physically and specifically with the macrophages.


Leukoplakia of the bladder: an 8-year follow-up.


Macrophage-lymphocyte clusters in the immune response to soluble protein antigen in vitro. II. Ultrastructure of clusters formed during the early response. Macrophage-lymphocyte clusters are formed when lymph node cells and autologous peritoneal exudate cells from guinea pigs immunized with tubercle bacilli are cultured in the presence of purified protein derivative of tuberculin (PPD) for 20 h. We have studied the ultrastructure of these clusters employing transmission and scanning electron microscopy. The most simple macrophage-lymphocyte cluster consisted of one macrophage, one large central lymphocyte with a blastoid appearance attached to the macrophage with a broad area of contact, and from a few to more than 20 small peripheral lymphocytes attached to the central lymphocyte by their uropods. Some clusters were of more complex type, containing two or three macrophages or one macrophage with more than one central lymphocyte attached to the surface, but even in these clusters each peripheral lymphocyte was attached only to one central lymphocyte. By morphological criteria the peripheral lymphocytes were T lymphocytes.


Microradiographic study of the normal human vertebral body.


Factor VIII-related antigen in tissues detected by the indirect immunofluorescence technique.


Temporal effects of artificial induction of ovulation on the hypothalamo-hypophysial-ovarian system of the catfish, Heteropneustes fossilis (Bloch).


Editoral: Alpha-fetoprotein in primary carcinoma of the liver and other diseases.


Editoral: Bodies offered for research.

Editoral: Bodies offered for research.


Editoral: Postoperative immunosuppression.


Explorations of a systems approach to alcoholism. Clinical observations of a simulated drinking gang.


Restoration of immune competence in tolerant mice by parabiosis to normal mice. These studies demonstrate that mice tolerant to human gamma globulin (HGG) regain their ability to make antibody to HGG after parabiosis to normal mice. This can be demonstrated by enumeration of PFC in the spleens of both the normal and tolerant partners. Hemagglutinin titers of normal-tolerant parabionts, however, are exceptionally low; serum antibody appears to be neutralized by circulating HGG present originally in the serum of the tolerant partner. These data support the hypothesis that tolerance to HGG in mice is a "defective" state due to the absence of cells capable of responding to this antigen.


Editorial: Acids, bases, and nomograms.

Editorial: Acids, bases, and nomograms.


Editorial: Antibiotics for disease.

Editorial: Antibiotics for disease.


Editorial: Beta-blockade and size of acute myocardial infarction.


Editorial: Computer-assisted tomography of the brain.


Editorial: Conservative operations for diverticular disease.


Editorial: Little light on phototherapy.


Editorial: Epilepsy, schizophrenia, and limbic system.


Editorial: Only black grapes.

Editorial: Only black grapes.


Editorial: Nephrocalcinosis and renal tubular acidosis.


Editorial: Optic neuritis of obscure origin.


Editorial: Progress with an artificial liver.


Editorial: Proliferating and phagocytic histiocytes.


Editorial: Self-injury.

Editorial: Self-injury.


Editorial: Sloth's compensations.

Editorial: Sloth's compensations.


Editorial: Social-work support for the Health Service.


Editorial: Scoliosis.

Editorial: Scoliosis.


Editorial: The patients we don't see.

Editorial: The patients we don't see.


Editorial: Starling's law survives.

Editorial: Starling's law survives.


Editorial: The pill off prescription?

Editorial: The pill off prescription?


Editorial: The specialty of infectious diseases.


Editorial: Viruses and heart-disease.

Editorial: Viruses and heart-disease.


Desmosome-like structures in acantholytic epidermal cells of contact dermatitis.


A glossary of terms most commonly used by clinical electroencephalographers.


Bullous scabies.

Bullous scabies.


The peribrachial region of cat. I. A topographical study with special reference to the locus coeruleus.


The pharmacology and clinical evaluation of aprindine a new antiarrhythmic agent.


The phenomenon of premature chromosome condensation: its relevance to basic and applied research.


Polyphosphate bone scans, 32phosphorus, and adenocarcinoma of the thyroid.


Cytophotometric factors causing apparent differences between Feulgen DNA contents of different leukocyte types.


Atrophy of the stria vascularis, a common cause for hearing loss.


The physiological role of gamma-globulin.


Whipple's disease. George Hoyt Whipple, (born 1878).


Paraplegia in carcinoma of the prostate.


Ectopic subvesical prostatic tissue.

Ectopic subvesical prostatic tissue.


The preparation of matrix-bound proteases and their use in the hydrolysis of proteins.


The prognostic importance of premature ventricular complexes in the late post-infarction period. Experience in the Cornary Drug Project.


The prognostic significance of ventricular premature contractions in healthy people and in people with coronary heart disease.


The properties of ionic channels measured by noise analysis in thin lipid membranes.


Asymptomatic arteritis of the uterine cervix. A necrotizing arteritis in the uterine cervix is described as an incidental finding in surgical material from 10 patients. The histological features are compared with those seen in polyarteritis nodosa. Subintimal hyaline deposition and a relative paucity of neutrophil and eosinophil polymorphs characterized the lesion but the histological appearance was never sufficiently specific to exclude confidently the possibility of polyarteritis nodosa. None of the patients had evidence of multisystem disease either at the time of operation or at subsequent follow-up assessment.


Receptor sites for aggregated gammaglobulin (AGG) on lymphocytes in lymphoproliferative diseases.


Lower-oesophageal-sphincter relaxation is a myth.


The pyridine-nucleotide transhydrogenase of Salmonella typhimurium.


Contractile structures in endothelial cells of splenic sinusoids.


The radioallergosorbent test (RAST): diagnostic and clinical significance.


Lateral amygdala unit acitvity: I. Relationship between spontaneous and evoked activity.


Lateral amygdala unit activity: II. Habituating and non-habituating neurons.


General and specific patterns of acrocentric association in parents of mongol children.


General aspects of anti-arrhythmic treatment with aprindine.


Propranolol in the surgical management of thyrotoxicosis. Forty-nine thyrotoxic patients prepared for partial thyroidectomy with the beta-adrenoceptor blocking drug, propranolol, and iodine are compared with 42 patients prepared with carbimazole and iodine. The age and sex distribution of the two groups were comparable, but patients with obstructive airways disease and possible cardiac insufficiency were excluded from preparation with propranolol. The mean duration of preoperative treatment with propranolol was 40 days, compared with 89 days for carbimazole. Propranolol treated patients had lower pulse rates before and after operation. The serum PB(127)I values immediately before and after operation were higher in the propranolol group than in the carbimazole group, but were the same in both groups one and four months after operation. The incidence of hypothyroidism at one year after operation was 30% in the carbimazole prepared patients and 31% in the propranolol patients. Serum calcium levels were higher in the propranolol group at the time of operation. No adverse effects from the use of propranolol and at operation the thyroid gland prepared with propranolol was firmer, less friable, more easily mobilised and less likely to bleed than the gland prepared with carbimazole. There is, consequently, less risk of damage to the parathyroid glands and recurrent laryngeal nerves. However, the basal metabolic rate remains high on propranolol therapy and very careful supervision is advised.


Generalized mastocytosis.

Generalized mastocytosis.


Alteration of keratinization in mouse ear epidermis in recombinant grafts with tail dermis.


Separation of water soluble proteins in psoriatic scales with different polyacrylamide gel concentrations and molecular weight estimations of the separated bands by disc-electrophoresis.


Ulcerative lichen planus of the feet. A case in which the serological findings suggested systemic lupus erythematosus.


Hypercalcemia associated with gaint condyloma accuminatum.


Alterations in serum proteins of rats after immunization with Freund's adjuvant and Bordetella pertussis vaccine.


The reaction of rheumatoid anti-Gm antibodies with native and aggregated Gm-negative IgG.


Age-related variation in proportion of circulating T cells.


The repression of medical science in absolutist Spain: the case of Juan Manuel de Aréjula 1755-1830.


The retrograde intraaxonal transport of horseradish peroxidase in the chick visual system: a light and electron microscopic study.


Allergy versus clinical immunology: a critical analysis.


Experimental hepatic porphyria induced by hexachlorobenzene as a model for human symptomatic porphyria.


Inherited metabolic disease.

Inherited metabolic disease.


International Federation of Societies for Electroencephalography and Clinical Neurophysiology.


Determination of the defective function in two mutants of Rous sarcoma virus.


Scleroderma in the child.

Scleroderma in the child.


Heterogeneity of carcinoembryonic antigen and its fractionation by con A affinity chromatography.


Control of diesel exhaust odors.

Control of diesel exhaust odors.


The role of langerhans cells in contact allergy. I. An ultrastructural study in actively induced contact dermatitis in guinea pigs.


Nephrotoxic canine glomerulonephritis.

Nephrotoxic canine glomerulonephritis.


Chloroplast and cytoplasmic ribosomes of Euglena: selective binding of dihydrostreptomycin to chloroplast ribosomes. Dihydrostreptomycin binds preferentially to chloroplast ribosomes of wild-type Euglena gracilis Klebs var. bacillaris Pringsheim. The K(diss) for the wild-type chloroplast ribosome-dihydrostreptomycin complex is 2 x 10(-7) M, a value comparable with that found for the Escherichia coli ribosome-dihydrostreptomycin complex. Chloroplast ribosomes isolated from the streptomycin-resistant mutant Sm(1) (r)BNgL and cytoplasmic ribosomes from wild-type have a much lower affinity for the antibiotic. The K(diss) for the chloroplast ribosome-dihydrostreptomycin complex of Sm(1) (r) is 387 x 10(-7) M, and the value for the cytoplasmic ribosome-dihydrostreptomycin complex of the wild type is 1,400 x 10(-7) M. Streptomycin competes with dihydrostreptomycin for the chloroplast ribosome binding site, and preincubation of streptomycin with hydroxylamine prevents the binding of streptomycin to the chloroplast ribosome. These results indicate that the inhibition of chloroplast development and replication in Euglena by streptomycin and dihydrostreptomycin is related to the specific inhibition of protein synthesis on the chloroplast ribosomes of Euglena.


Robertsonian translocation between the chromosome Y and 15.


Instruction in oral hygiene--a group approach.


Observations on axoplasmic transport in rabbits with aluminum-induced neurofibrillary tangles.


Observations on carcinoma of the oesophagus.


Mucus catheter with a protective guard.

Mucus catheter with a protective guard.


Observations on the use of solid-phase-coupled antibodies in the radioimmunoassay of human placental lactogen. A detailed comparative assessment was made of the use of solid-phase-coupled antibodies in radioimmunoassay, by using an assay for human placental lactogen as a model system. The major advantages of the solid-phase technique are: (1) in common with the use of a second antibody, it is universally applicable; (2) separation can be carried out rapidly; (3) in contrast with some other techniques, the separation of antibody-bound and free antigen is virtually complete. The disadvantages when compared with other procedures are: (1) a considerable proportion of the antibody may be lost during the initial coupling reaction; (2) the tubes must be continuously mixed during incubation, and much effort is expended in removing and replacing the caps; (3) there is a decrease in the apparent affinity constant of the antibody after coupling, which is reflected in a lower sensitivity of the assay system. It is concluded that solid-phase antibodies are of greatest value in those systems in which the supply of antiserum is abundant, and in which the achievement of high sensitivity is not a requirement.


Tests for penicillin allergy in man. II. The immunological cross-reaction between penicillins and cephalosporins.


Class specificity of intracellular and surface immunoglobulin of cells in popliteal and intestinal lymph from sheep.


Argyrophil mucus-secreting adenocarcinoma of the colon and sebocystomatosis: report of a case.


Warts. Biology and current therapy.

Warts. Biology and current therapy.


Lupus-like syndromes induced by drugs.

Lupus-like syndromes induced by drugs.


Cross-reactivity of synthetic linear dextran with anti-B512 dextran. Viewpoints on the nature of the antigenic determinants of dextran.


Cross-reactivity of the axial filament antigen as a criterion for classification of Leptospira.


Giemsa banding of 'normal' leukaemic chromosomes: a preliminary report.


Polysaccharide surface coat (glycocalix) of human placental villi.


A case of brain fever.

A case of brain fever.


A case of irreversible bilateral optic damage after ethambutol therapy.


The severe form of alpha thalassaemia is caused by a haemoglobin gene deletion.


Transplantation and the ageing kidney.

Transplantation and the ageing kidney.


Non-specific stimulation of immunoglobulin synthesis in mice by capsular polysaccharide of Klebsiella pneumoniae.


Assessment of cadaveric kidneys for transplantation.


Remission of familial goitrous hypothyroidism.


Assessment of human renal transplantation.


A post-hysterectomy syndrome.

A post-hysterectomy syndrome.


Cryosurgery of the prostate, a four years follow-up study.


Resistotyping of Proteus mirabilis and a comparison with other methods of typing. Resistotyping of P. mirabilis using 10 compounds is reported. The method was tested for reproducibility and specificity and results were compared with those obtained by serological, bacteriophage, and proticine typing methods and the Dienes test. The possible relationship between resistance to the chemicals used in the test and antibiotics was also studied.The method was found to be simple, reproducible, show good specificity, and compare favourably with other typing methods. No linkage between resistance to the chemicals and antibiotic resistance was detected.


The structure and growth of valve-pocket thrombi in femoral veins. The structure of 50 small thrombi in femoral valve pockets and the microscopic contents of 35 apparently empty pockets were studied in an attempt to ascertain the nature of the microscopic nidi from which thrombi form and their manner of growth to visible thrombi. Sixteen thrombi had little or no cellular invasion. Most of these recent structures had two main regions, red areas restricted distally in the pocket by the vein wall, and larger white regions comprising most of the thrombus length and often covering the red areas. Red areas are the early sites of cellular adhesion and invasion and the likely sites of origin of most thrombi. They were usually dominated by red cells and fibrin. White zones, which represent propagation growth, are characterized by many foci of platelets with fibrin borders (platelet-fibrin units). Some red areas also contained platelet-fibrin units but they were few and tiny; platelets were not seen in others and one small wholly red thrombus was devoid of platelets. Degenerative changes in platelet-fibrin units were observed, and it is postulated that many become purely fibrin structures. There was no significant evidence of preceding intimal damage in the vein wall. Therefore nidi are laid down on normal endothelium probably on the vein wall near the apex of the pocket. Some pockets, empty of thrombi, contained condensed foci of red cells or tiny fibrin fragments surfaced by endothelial cells and considered to be the remnants of aborted thrombi; a few contained clumps of platelets or leucocytes. It is postulated that any of these may represent the nidi from which thrombi grow. Several thrombi also incorporated large fat droplets, numerous in two. Fat embolic globules derived from fractures are their likely source.


The surgical significance of thyroid isotopology: morphologic and physiologic considerations.


Light, scanning, and transmission electron microscopy of stable staphylococcal L-forms.


Unintegrated murine leukemia viral DNA in newly infected cells.


The termination of immunologic unresponsiveness to the cyanogen bromide fragments of bovine serum albumin in rabbits.


Growth responses of selected quail lines to goitrogenic stresses.


Endothelial cell morphology in focal areas of in vivo Evans blue uptake in the young pig aorta. I. Quantitative light microscopic findings.


Genetic control of responses to bacterial lipopolysaccharides in mice. I. Evidence for a single gene that influences mitogenic and immunogenic respones to lipopolysaccharides. In vivo immune responses and in vitro mitogenic responses to bacterial lipopolysaccharides (LPS) have been compared in strains of C3H mice. C3H/HeJ spleen cultures did not support mitogenic responses to LPS and in vivo these mice produce low IgM responses to LPS. On the basis of these two responses, C3H/HeJ mice have been termed low LPS responders. All other strains of C3H mice tested (C3HeB/FeJ, C3H/DiSn, C3H/Str, CWB, CSW, and C3H/Sf and its H-2 congenics) are high LPS responders supporting large in vitro mitogenic and in vivo immune responses to LPS. The immune response difference between low and high LPS responders is a quantitative one. IgM responses are observed in C3H/HeJ mice in the range of 1.0-10 microg LPS. At lower and higher LPS concentrations, immune responses are not observed. In contrast, high LPS responders elicit LPS immune responses over a much wider dose range (0.1-200 microg). The ability to respond well to LPS is dominant as shown by the response of F(1) hybrid mice of low responder and high responder strains. The linkage relationships of mitogenic and immune responsiveness to LPS have been investigated in backcross (C3H/HeJ x CWB)F(1) x CWB mice. All mice that gave in vivo immune responses to LPS also supported mitogenic responses to LPS. The defect in C3H/HeJ mice that limits mitogenic and immune responsiveness to be due to a single autosomal gene which is not linked to the H-2 histocompatibility or heavy-chain allotype loci.


Practical aspects of the hepatitis B problem.


Genetic mapping of the antigenic determinants of two polysaccharide K antigens, K10 and K54, in Escherichia coli. The genes controlling synthesis of the Escherichia coli acidic polysaccharide capsular antigens K10 and K54 were transferred by conjugation to E. coli strains of other serotypes. The genes concerned with these K antigen determinants showed genetic linkage with the serA locus. We propose to name the K antigen-controlling gene kpsA. The genetic determinants of the two K antigens could also be transferred to enteropathogenic serotypes, even though such strains have never been found in nature with special acidic polysaccharide K antigens. A noncapsulated derivative, K(-), of the K10 strain can transfer the genetic determinant of the K antigen, demonstrating the probable existence of another chromosomal locus involved in the production of such acidic polysaccharide K antigens.


Prostate cancer.

Prostate cancer.


Identification by peroxidase staining of monocytes in surface immunofluorescence tests.


Identification of chloroplast coupling factor by freeze-etching and negative-staining techniques. Identification of chloroplast coupling factor particles, by the freeze-etching and negative-staining techniques, was made utilizing chloroplast thylakoids isolated from spinach leaves. Complete removal of particles, comparable in diameter to purified coupling factor particles, from the outer surface of freeze-etched thylakoids was achieved by treatment with 0.8% silicotungstate. Reappearance of particles, comparable in diameter to purified coupling factor particles, on the outer surface of freeze-etched thylakoids was demonstrated by combining silicotungstate-treated thylakoids with purified chloroplast coupling factor. Negative-staining results were in agreement with the freeze-etch data. The results demonstrate that the chloroplast coupling factor particles are exposed on the outer surface.


Identification of discrete classes of normal human peripheral lymphocytes by multiparameter flow analysis.


Identification of monoaminergic fluorescent neurons by azan-staining in the cerebral ganglion of the earthworm, Allolobophora caliginosa.


Identification, enumeration, and isolation of B and T lymphocytes from human peripheral blood. Report of a WHO-IARC-sponsored workshop on human B and T cells, London, 15-17 July 1974.


Functional cellular maturation in cultures of human haematopoietic cells.


Fixation with mercury salts.

Fixation with mercury salts.


Quantitative thin-layer gel filtration applied to gamma-globulin preparations.


Functional changes of the neuronal and glial elements at the surface of the external layer of the median eminence.


Reactivity of steroid-resistant neonatal thymocytes.


Treatment of chronic ventricular dysrhythmias with a new drug: aprindine (AC 1802).


Monitoring odorous air pollution in the field with human observers.


Endotoxin-induced intravascular coagulation and shock in dogs: the role of factor VII.


The treatment of bacterial endophthalmitis following retinal surgery.


Electroencephalographic activity of caimans as related to diving.


Electron microscopic observations of melanin transfer in human skin. Evidence for indirect melanin transfer.


Electron translucent vesicles in adrenal medulla following catecholamine depletion.


Penetration of pollutants in the airways.


Electrophoretic pattern of equine cerebrospinal fluid.


Electrophysiologic basis of arrhythmias: a clinical correlation.


Idiotypic uniformity of cell surface immunoglobulin in chronic lymphocytic leukeima. Evidence for monoclonal proliferation.


Serotyping of adenoviruses using immune electron microscopy. Immune electron microscopy may be used to determine the type specificity of several common human adenoviruses. The method described utilized equine antisera and was used to serotype adenoviruses within hours after typical cytopathic effect was observed in tissue culture. Adenovirus types 1, 2, 3, 5, 7a, 8, and 11 were isolated from clinical specimens and serotyped by serum neutralization and immune electron microscopy. Five of the seven types were identified consistently and identically by the two methods. Types 7a and 11 were indistinguishable by immune electron microscopy.


Substructures and polypeptides of Visna virus. The protein of Visna virus, disrupted by 8 M guanidine hydrochloride and heating, was resolved into 10 polypeptides by agarose gel column chromatography in 6 M guanidine hydrochloride. Two of the peaks contained glycopolypeptides. Nonidet-disrupted virions were resolved into two fractions by potassium tartrate gradient centrifugation, with densities of 1.08 and 1.24 g/ml, respectively. About 70% of the viral DNA polymerase directed by added template was released into the light fraction, in which very little endogenous enzyme activity was detected. Also released into the light fraction were all of the glycopolypeptides, 50% of the viral RNA, and a part of each of the other viral protein components. The data indicate that extensive degradation of subviral structures occurred, even under mild conditions for virion disruption. The 1.24-g/ml fraction was composed of 50% of the viral RNA, most of the endogenous DNA polymerase activity (80%), and a major internal polypeptide (GuHCl6) with an estimated mol wt of 28,000. Two other polypeptides were also consistently detected in the heavy fraction, but they constituted less than 25% of the ribonucleoprotein complex, compared with 75% for GuHCl6.


Unusual inclusions occurring in the blasts of four patients with acute leukemia and Down's syndrome.


Production of C and T bands in human chromosomes after heat treatment at high pH and staining with "stains-all".


A study of lead concentrations in blood of children (and some adults) of Southern Tasmania.


Tissue-culture microtest for predicting response of human cancer to chemotherapy.


Influence of hydralazine consumption on allylamine induced myocardial fibrosis and hypertrophy in rats.


Influence of glutaraldehyde and-or osmium tetroxide on cell volume, ion content, mechanical stability, and membrane permeability of Ehrlich ascites tumor cells. Effects of fixation with glutaraldehyde (GA), glutaraldehyde-osmium tetroxide (GA-OsO(4)), and osmium tetroxide (OsO(4)) on ion and ATP content, cell volume, vital dye staining, and stability to mechanical and thermal stress were studied in Ehrlich ascites tumor cells (EATC). Among variables investigated were fixation time, fixative concentration, temperature, osmolality of the fixative agent and buffer, total osmolality of the fixative solution, osmolality of the postfixation buffer, and time of postfixation treatment in buffer (Sutherland, R. M., et al. 1967. J. Cell Physiol.69:185.). Rapid loss of potassium, exchangeable magnesium, and ATP, and increase of vital dye uptake and electrical conductivity occurred with all fixatives studied. These changes were virtually immediate with GA-OsO(4) or OsO(4) but slower with GA (in the latter case they were dependent on fixative temperature and concentration) (Foot, N. C. 1950. In McClung's Handbook of Microscopical Technique. 3rd edition. 564.). Total fixative osmolality had a marked effect on cell volume with OsO(4) but little or no effect with GA or GA-OsO(4). Osmolality of the buffer had a marked effect on cell volume with OsO(4), whereas with GA or GA-OsO(4) it was only significant at very hypotonic buffer osmolalities. Concentration of GA had no effect on cell volume. Osmolality of the postfixation buffer had little effect on cell volume, and duration of fixation or postfixation treatment had no effect with all fixatives. Freezing and thawing or centrifugal stress (up to 100,000 g) had little or no effect on cell volume after all fixatives studied. Mechanical stress obtained by sonication showed that OsO(4) alone produced poor stabilization and that GA fixation alone produced the greatest stabilization. The results indicate that rapid membrane permeability changes of EATC follow fixative action. The results are consistent with known greater stabilizing effects of GA on model protein systems since cells were also rendered relatively stable to osmotic stress during fixation, an effect not noted with OsO(4). After fixation with GA and/or OsO(4) cells were stable to osmotic, thermal, or mechanical stress; this is inconsistent with several earlier reports that GA-fixed cells retain their osmotic properties.


Influence of passive smoking and parental phlegm on pneumonia and bronchitis in early childhood.


Cellular and humoral response against determinants other than the classical HL-A specificities. Evidence for at least one system independent of the major histocompatibility complex.


Cellular immune response to a drug-treated L5178Y lymphoma subline.


Capsule production and virulence among strains of Staphylococcus aureus.


The ultrastructure of calf heart conducting fibers with special reference to nexuses and their distribution.


Stimulation of Herpesvirus saimiri expression in the absence of evidence for type C virus activation in a marmoset lymphoid cell line. Nucleic acid base analogues were used to examine a Herpesvirus saimiri (HVS)-infected marmoset lymphoid cell line (MLC-1) for possible association with type C viruses. Synthetic templates poly(rA).d(pT)(10) and poly(dA).d(pT)(10) were used to detect RNA-directed DNA polymerase activity in 100-fold concentrated tissue culture fluids. HVS was monitored by immunofluorescence for early, late, and membrane antigens. MLC-1 cells were exposed to 30 mug of 5-bromo-2'-deoxyuridine (BUdR) per ml for 24 h and examined daily. Similar experiments used 5-iodo-2'-deoxyuridine (IUdR) (20 mug/ml) for 30 h or IUdR (20 mug/ml) for 3 days followed by 2% dimethyl sulfoxide for 4 days. Results of these experiments failed to show any type C virus-like polymerase; however, HVS expression was greatly stimulated. BUdR and IUdR enhanced expression of HVS-associated antigens five- to sevenfold, with maximal stimulation being observed 3 to 4 days after removal of the analogue. IUdR-dimethyl sulfoxide treatment was generally less effective. Although more cells showed HVS antigens, the treatments did not increase cell-free infectious virus. The data suggest that HVS-infected lymphoid cells can be stimulated to express virus in a manner similar to that of the Epstein-Barr virus in Burkitt's lymphoma cells. No evidence of type C virus was found in stimulated cultures.


The ultrastructure of lipid-depleted rod photoreceptor membranes. The structure of lipid-depleted retinal rod photoreceptor membranes was studied by means of electron microscopy. Aldehyde-fixed retinas were exhaustively extracted with acetone, chloroform-methanol, and acidified chloroform-methanol. The effect of prefixation on the extractability of lipids was evaluated by means of thin-layer chromatography and fatty acid analysis. Prefixation with glutaraldehyde rendered 38% of the phospholipids unextractable, while only 7% were unextractable after formaldehyde fixation. Embedding the retina in a lipid-retaining, polymerizable glutaraldehyde-urea mixture allows a comparison of the interaction of OsO(4) with lipid-depleted membranes and rod disk membranes which contain all their lipids. A decrease in electron density and a deterioration of membrane fine structure in lipid-depleted tissue are correlated with the extent of lipid extraction. These observations are indicative of the role of the lipid bilayer in the ultrastructural visualization of membrane structure with OsO(4). Negatively stained thin sections of extracted tissue reveal substructures in the lipid-depleted rod membranes. These substructures are probably the opsin molecules which are the major protein component of retinal rod photoreceptor membranes.


Arthur Henry Douthwaite.

Arthur Henry Douthwaite.


Failure of low-dose heparin to prevent deep-vein thrombosis after hip-replacement arthroplasty.


The use of a radioimmunoassay for alpha-fetoprotein in the diagnosis of malignancy.


Diffusion constant and dimension of bacteriophage phi X174 as determined by self-beat laser light spectroscopy and electron microscopy. The diffusion constant of bacteriophage phiX174 was determined by laser light self-beat spectroscopy. The method allows one to establish the diffusion constant in comparatively short time and with high accuracy. From the diffusion constant D(37) = 1.96 +/- 0.08 x 10(-7) cm(2)/s, the size of the virus particles within their aqueous milieu can be calculated. For phiX174, a diffusional diameter of 31.4 +/- 1.0 nm was found, in good agreement with measurements of diameters of freezeetched (32.3 +/- 1.8 nm) and negatively stained particles (33.8 +/- 2.1 nm), provided that the entire spikes of the virion are included. Other isometric viruses may show a complex interaction of the virion with the surrounding water and its ions.


The use of antinucleoside antibodies to probe the organization of chromosomes denatured by ultraviolet irradiation.


An automatic hybrid analyzer of sleep stages in the rat.


An autopsy case of Sandhoff's disease.

An autopsy case of Sandhoff's disease.


The visualization of concanavalin A binding sites in Purkinje cell somata and dendrites of rat cerebellum. The localization of concanavalin A (Con A) binding sites in Purkinje cell somata and dendrites has been studied using a peroxidase labeling technique. In the somata, the nuclear, Golgi, and endoplasmic reticulum (ER) membranes are rich in Con A binding sites. The hypolemmal cisternae, which are continuous with the ER from the soma and throughout the dendritic tree of Purkinje cells, are also rich in Con A binding sites. Other cisternae seen in these dendrites do not bind detectable amounts of Con A. The results suggest that a cisternal system, rich in carbohydrate, may be continuous from the nuclear envelope to distal dendritic segments of Purkinje cells. Such a system could play a role in the movement of materials from Purkinje somata to dendrites.


A new adenovirus from sheep.

A new adenovirus from sheep.


A new method for the quantitative detection of platelet aggregates in patients with arterial insufficiency.


8. Two simple but useful methods for the analysis of dyes: gel-filtration chromatography and solvent extraction.


A sex difference in plasma thyroxine and thyroid stimulating hormone in rats.


An experimental study of acute neonatal enterocolitis--the importance of breast milk.


Study of the cytoplasmic lipids in thymic involution.


Early structural changes in a case of late infantile amaurotic idiocy (early form of Batten-Spielmeyer-Vogt disease).


X-ray studies on antibody fragments.

X-ray studies on antibody fragments.


Ten years experience on prostate cryosurgery.


Embryonic antigens in tumor immunity.

Embryonic antigens in tumor immunity.


Analyses of lymphocytes from patients with rheumatoid arthritis and systemic lupus erythematosus. Occurrence of interfering cold-reactive antilymphocyte antibodies. Large percentages of the lymphocytes from some patients with rheumatoid arthritis and systemic lupus erythematosus were densely covered with Ig demonstrable by immunofluorescence, which was occasionally present in the form of caps. The amount and character of the Ig staining depended largely on the procedures used in the isolation and washing of the lymphocytes. Cold-reactive antilymphocyte antibodies present in many sera wre primarily responsible for these variations. Overnight culture of the lymphocytes proved to be an efficient procedure for the removal of adsorbed antibody. Some evidence was also obtained for the presence of circulating immune complexes and exogenous rheumatoid factor molecules on the lymphocyte surface. Thus on freshly isolated cells the demonstration of surface Ig proved to an unreliable marker of bone marrow-derived (B) cells in these disease: the actual percent of B cells with intrinsic surface Ig was often markedly decreased. In patients with systemic lupus erythematosus, this reduction was in agreement with the low numbers of cells that had a receptor for aggregated IgG. The mean percentage of thymus-derived (T) cells in both diseases was slightly greater than the normal level.The concentrations of lymphocytes in joint fluids from patients with rheumatoid arthritis were often greater than levels found in blood. T cells primarily accounted for this increase. The T cells typically formed unusually dense rosettes with sheep erythrocytes. B lymphocytes were proportionally much diminished. Evidence was obtained for the existence of a major joint fluid lymphocyte population that lacked all assayed surface markers.


Tye B virus and human breast cancer.

Tye B virus and human breast cancer.


Schizophrenia and the limbic system.

Schizophrenia and the limbic system.


The CLARA hematoxylin reaction.

The CLARA hematoxylin reaction.


The Ea-2 (H-14) cellular antigen system in the mouse.


New Giemsa method for the differential staining of sister chromatids.


An ATPase from dog gastric mucosa: changes of outer pH in suspensions of membrane vesicles accompanying ATP hydrolysis.


S-100 protein in synapses of the central nervous system.


Temperature-dependent properties of gramicidin A channels.


Cytodiagnostic use of Feulgen-DNA measurements in cell imprints from the skin of patients with mycosis fungoides.


High resolution negative staining of ferritin molecules on vermiculite single crystal supports.


Nitrocellulose filter retention method for studying drug-nucleic acid interactions.


Erythroid colony formation in vitro by dimethylsulfoxide-treated erythroleukemic cells.


Methemoglobin reductase (cytochrome b5 reductase) deficiency in congenital methemoglobinemia.


Immunochemical identification and characteristics of a specific antigen of the human retina.


In vivo tests of thyroid function.

In vivo tests of thyroid function.


Letter: Tests of thyroid function.

Letter: Tests of thyroid function.


Surface structures of the gonococcus.

Surface structures of the gonococcus.


Tonsillar gonorrhoea demonstrated by a suction device.


Alfred François Donné, 1801-1878, discoverer of Trichomonas vaginalis and of leukaemia.


Topography of retinal efferent connections in sciurids.


Retrograde transport of horseradish peroxidase in transected axons. 1. Time relationships between transport and induction of chromatolysis.


Constitutive heterochromatin of Indian muntjac chromosomes revealed by DNAse treatment and a C-banding technique.


Specific C band patterns in continuous human lymphoblastoid cell lines.


A study of prostaglandins and prostaglandin antagonists in relation to anaphylaxis in calves.


Transport of succinate in Escherichia coli. III. Biochemical and genetic studies of the mechanism of transport in membrane vesicles.


Response of alpha-fetoprotein to chemotherapy in patients with hepatomas.


Alpha-fetoprotein and germ cell tumors: a possible role of yolk sac tumor in production of alpha-fetoprotein.


The pathogenesis of Hyostrongylus rubidus in growing pigs.


Potentiation of bleomycin by an antifungal polyene, pentamycin, in transformed animal cells.


Bleomycin-induced damage in prematurely condensed chromosomes and its relationship to cell cycle progression in CHO cells.


Characterization of a yeast D-mannan with an alpha-D-glucosyl phosphate residue as an important immunochemical determinant.


Monoaminergic tracts of the diencephalon and innervation of the pars intermedia in Rana temporaria. A fluorescence and microspectrofluorimetric study.


The effect of change of background colour on the ultrastructure of the pars intermedia of the pituitary of the eel (Anguilla anguilla).


Ultrastructural changes of the oenocytes of Gryllus bimaculatus DEG (Saltatoria, Insecta) during the moulting cycle.


Specific apocrine secretion in the anterior lobe of the prostate gland of rabbits.


Comparison of 5-azacytidine (NSC-102816) with CCNU (NSC-79037) in the treatment of patients with breast cancer and evaluation of the subsequent use of cyclophosphamide (NSC-26271).


Structure of synthetic and native myosin filaments from Amoeba proteus.


Some observations on the ultrastructure of the central amygdaloid nucleus in the cat.


Hypothalamic projections to the amygdala in the cat. A light and electron microscopic study.


Treatment of experimental mammary adenocarcinoma with herbicides.


Failure of indium 111-labeled bleomycin tumor scanning to predict response  to bleomycin (NSC-125066) treatment.


Characterization of anti-heart mitochondria autoantibodies produced in dogs following myocardial infarction.


The pathophysiology of malignant ventricular arrhythmias during acute myocardial ischemia.


Demonstration of re-entry within the His-Purkinje system in man.


The in vivo effect of theophylline on histamine release from human leucocytes.


DDT, dieldrin, and heptachlor epoxide in humans with liver cirrhosis.


The radioimmunoassay of thyroxine in unextracted human serum.


Pattern of Y-chromatin fluorescence in an XYY man.


Y-chromatin fluorescence in human buccal smear.


Longer Y chromosome in criminals.

Longer Y chromosome in criminals.


Enterohepatic cycling of thyroxine in starved and fed brook trout, Salvelinus fontinalis (Mitchill).


DNA helical content during the C-banding procedure.


Relative activation and inactivation phenomena between homologous and nonhomologous nucleolus organizers on the normal human G chromosomes.


A (6;21) translocation, unbalanced, 46 chromosomes. Repository identification no. GM-144.


Karyotypic analysis of a near-diploid established mouse cell line.


An (X;1) translocation, balanced, 46 chromosomes. Repository identification no. GM-97.


Treatment of pulmonary tuberculosis.

Treatment of pulmonary tuberculosis.


Simultaneous cure of thyrotoxicosis and Wolff-Parkinson-White syndrome.


His bundle parasystole: A form of A-V junctional parasystole.


Location of repetitious DNA in the chromosomes of the desert locust (Schistocerca gregaria).


Chromosome regions containing DNAs of known base composition, specifically evidenced by 2,7-di-t-butyl proflavine. Comparison with the Q-banding and relation to dye-DNA interactions.


Examinations of the gomori-positive cells of the thymus in tissue culture.


Life stage and secretory cycle of anterior pituitary basophils.


Relation between syringomyelia and von Hippel-Lindau's disease.


CSF-protein patterns in extrapyramidal diseases. Preliminary report with special reference to the protein patterns in Huntington's chorea.


A new metabolic disorder: myopathy with glycosamino(sialo)glycans accumulation.


The uptake, distribution and anti-tumor activity of bleomycin in gliomas in the mouse.


Sinus histiocytosis with massive lymphadenopathy--properties of cultured histiocytes.


Effect of bleomycin on the sheep erythrocyte-induced rossette formation and on the allograft survival in mice.


Papain-resistant (f) and papain-sensitive (g) subclasses of rabbit sLgA. Allotypic specificities on different domains of the g subclass of alpha-chains (alpha g).


The preparation and purification of HL-A monospecific antigens using platelets and various lymphoid sources.


The generation of antibody diversity. II. Plaque morphology as a simple marker for antibody specificity at the single-cell level.


Localization and structural features of an antigenic determinant in sea anemone collagen.


Immunogenicity and antigenic specificity of the loop fragment of lysozyme.


Modification of culture medium for Xenopus embryonic cells. I. Effects of albumin and other proteins.


Association of antibody to nerve growth factor with ganglionic non-neurons (glia) and consequent interference with their neuron-supportive action.


The nuclear DNA content and chromatin ultrastructure of the coelacanth Latimeria chalumnae.


Reactivation of nuclear RNA polymerase activity in growth stimulated epithelial cells.


Rapid techniques for counting and analysis of chromosome aberrations.


Ocular antigens. V: Species specificity of structural glycoproteins isolated from ocular connective tissues. An immunofluorescence and immunochemical study.


The presence of a polyadenylic acid-dependent DNA polymerase in calf lens.


Numerical increase of Gomori-positive glia in rat optic nerve following x-irradiation.


Ocular antigens. VI: Immunogenic determinants in ocular and non-ocular tissues related to uveal pigment granules. An immunofluorescence and immunodiffusion study.


Rôle of nerve-muscle contact in maintaining synaptic connections.


Schistosoma mansoni: defined system for stepwise transformation of cercaria to schistosomule in vitro.


Unusual lysosomes in hamster hepatocytes.


Selective degeneration of central dopaminergic systems after injection of 6-hydroxydopamine in the ventral mesencephalic tegmentum of the rat. Demonstration by the Fink-Heimer strain.


Reaction mechanisms in the release of mediators of immediate hypersensitivity from human lung tissue.


Retrograde axoplasmic transport: its continuation as anterograde transport.


Novel inhibitors of RNA synthesis.

Novel inhibitors of RNA synthesis.


Chemistry and mechanism of action of bleomycin.


Effect of anti-sperm antibodies on oxygen utilization and lactic acid production by human spermatozoa.


Energy dispersive x-ray analysis: application to assessment of spermatoan viability in primate semen.


Systemic lupus erythematosus and lupus renal disease. Diagnosis and management.


Determination by radioimmunoassay of the luteinising hormone-releasing hormone (LHRH) content of the hypothalamus of the rat and some lower vertebrates.


Quantitative determination of aggregated IgG in gamma globulin preparations.


The effect of phenformin plus stanozolol on fibrinolysis, platelet adhesiveness and blood lipids in patients with recurrent venous thrombosis.


Monocytic and lymphocytic origin of macrophages (preliminary communication).


Improvement in the cytochemical localization of aryl sulphatase in bone marrow and blood cells by use of naphtyl-sulphate and naphtol-AS-BI-sulphate.


Platelet functions and ultrastructure in the Hermanský-Pudlák syndrome.


Structure and function of peripheral blood leukocytes from patients with elevated steroid level in organism. I. Electron microscopic and cytochemical studies of leukocytes of peripheral blood in Cushing's syndrome.


Human CGL bone marrow cells grown in vitro, their immunological, cytological and cytochemical characteristics and attempts of their xenotransplantation.


The tissue plasminogen activator and plasma fibrinolysis in diet induced lipemia in rats.


Thrombohaemorrhagic skin lesion induced by a single injection of neuraminidase (EC. 3.2.1.18) in rabbits.


Applications of the fluorescence technique of Falck-Hillarp in studies on connections in the central nervous system.


Formation of messenger RNA in animal cells.


14S mRNA of immunoglobulin light chain: purification, translation, and transcription into cDNA.


Binding of a phallotoxin to protein filaments of plasma membrane of liver cell.


Family therapy training: a statewide program for mental health centers.


G-band analyses of an established cell line of a human malignant glioma.


Pericentric inversion of "fluorescent" segment in chromosome no. 3.


Different forms of incomplete trisomy 13. Mosaicism and partial trisomy for the proxim.


Subdivision of the human Y chromosome.

Subdivision of the human Y chromosome.


Studies on the mechanism of chromosome banding with trypsin.


Serum polymorphisms in Down's syndrome.

Serum polymorphisms in Down's syndrome.


Comparison of glycoproteins isolated by phenol-water partition of human erythrocyte membranes.


Inherited (13;14) translocation and reproduction. Report on three families.


Linear and nonlinear HL-A9 subdivisions generated by serial dilution.


Activity of mouse reaginic antibodies in the rat: passive cutaneous anaphylaxis and in vitro passive histamine release.


Platelet aggregation as a quantitative immunologic technique.


Contact sensitivity to oxazolone in the mouse. VIII. Demonstration of several classes of antibody in the sera of contact sensitized and unimmunized mice by a simplified antiglobulin assay.


Photometric evaluation of indirect immunofluorescence chessboard titrations for the characterization of FITC-labelled antibodies.


The optical rotatory dispersion of antigen-antibody complexes.


Hydrational aspects of A-anti-A and B-anti-B interactions.


Effect of non-ionic polymers on the formation of immuno-precipitates in single radial immunodiffusion techniques.


A physiological mechanism preventing experimental allergic encephalitis.


Polymorphism and intraspecific differences among spermatozoan and seminal plasma antigens.


Subtypes of hepatitis B antigen.

Subtypes of hepatitis B antigen.


Effects of antigen-antibody complexes on the primary immune response in Rhesus monkeys. A model for the enhancement of the primary humoral immune response of rhesus monkeys to marginal or weakly antigenic vaccines is presented. Our procedure used the complexing of formalin-inactivated Venezuelan equine encephalomyelitis (VEE) virus vaccine with specific, homologous, immune gamma globulin (IgG) at equivalence. Equivalence was determined by combining the concentrated, isotopically labeled ((3)H) virus with various concentrations of specific Sephadex-fractionated IgG. Enhancement of VEE virus antibody response in monkeys was obtained from preparations containing a marginal concentration of antigen that was complexed at equivalence with homologous IgG as compared with antigen alone. Protection in Swiss mice closely paralleled the antibody response pattern observed in monkeys, since complexes at equivalence provided 30 to 50% greater protection against challenge than antigen alone.


Effect of synthetic disaccharide-protein conjugate as an immunogen in Salmonella infection in mice. The synthetic disaccharide 3-O-alpha-tyvelopyranosyl-alpha-D-mannopyranoside, identical to the O antigen 9 determinant in Salmonella, covalently linked to bovine serum albumin and suspended in Freund complete adjuvant, was used as an immunogen in mice. Antibodies in high titer were formed against the O9 determinant as estimated by a bactericidal assay, whereas only low titers of hemagglutinating antibodies were found. A sensitive in vivo assay demonstrated that in immunized mice, after challenge with two strains of equal virulence but with different O antigens (O9, 12 and O4, 5, 12, respectively), the growth of the strain with the O9 determinant was suppressed in comparison to that with the O4 determinant.


Measurement of intracellular iodophilic polysaccharide in two cariogenic strains of Streptococcus mutans by cytochemical and chemical methods. Quantitative cytological and chemical methods have been developed to study the intracellular iodophilic polysaccharide (IPS) content of two strains of Streptococcus mutans. The cytological method uses a periodic acid-chlorite treatment of thin sections to increase the affinity of IPS for uranyl and lead salts. This results in the IPS appearing as individual electron-dense granules which can be counted for quantitative studies. As a basis for these quantitative studies, IPS was measured chemically by dissolving whole cells with hot KOH and quantitating spectrophotometrically the amount of iodine-polysaccharide complex formed.


Antigenic relationships among Newcastle disease virus mutants obtained from laboratory strains and from recent California isolates. The antigenic relationship between pairs of plaque mutants of Newcastle disease virus (NDV) derived from laboratory strains and from isolates from the 1971-72 California epizootic were examined by kinetic neutralization test. Comparing four sets of mutants from laboratory strains by both kinetic neutralization and hemagglutination inhibition tests, a similarity was found in the antigenic relationship expressed as an r value with both tests. However, kinetic neutralization was the more precise as well as sensitive assay. Antigenic diversity was greatest between pairs of mutants from different strains, but distinctions could also be made between mutants from the same strains such as Herts-L and Herts-S with an r value of 36%. Examination of mutants from the California epizootic isolated from separate locations and at different times showed antigenic divergence which was greatest between two red-plaque mutants with an r value of 39%. Antigenic distinctions were found between a red- and clear-plaque mutant obtained from isolates taken from brain and tracheal swabs of one infected chicken. In addition to antigenic divergence found between pairs of some mutants, two of the clear-plaque mutants reacted more avidly with antibody than did the corresponding red-plaque partner. Thus, both differences in antigenicity and avidity can be found among these NDV mutants. The antigenic variation found among these mutants is similar to that found within a serotype. This would imply that at the present NDV is a single serological type.


New type of exfoliatin obtained from staphylococcal strains, belonging to phage groups other than group II, isolated from patients with impetigo and Ritter's disease. Four strains of Staphylococcus aureus of a phage type other than the second group, isolated from patients with impetigo and Ritter's disease, were found to produce an exotoxin similar to those reported by Melish et al. (1972), Kapral and Miller (1971), and Arbuthnott et al. (1973). This toxin could elicit a general exfoliation of the epidermis with the so-called Nikolsky sign when subcutaneously inoculated into neonatal mice within 4 days after birth. The new toxin was serologically different from exfoliatin produced by the phage group II staphylococci previously reported (Kondo et al., 1973) and showed an electrophoretic pattern corresponding to that of the B-type toxin of the latter in acrylamide disc electrophoresis. It had the same molecular weight as that of the latter, which was estimated to be about 24,000. It was thermolabile and lost its toxic activity by heating at 60 C for 30 min; in addition, most of the toxicity was lost within 1 month of storage even at -30 C. We propose to designate the old typical heat-stable exfoliatin as S. aureus exfoliatin A and the new heat-susceptible exfoliatin as S. aureus exfoliatin B.


Ultrastructure of pili and annular structures on the cell wall surface of Neisseria meningitidis. In a survey of negatively stained preparations of the prototype strains of Neisseria meningitidis, pili were detected on three strains. However, these pili were detected on fewer than 5% of cells in populations of these three strains. Those individual cells with pili were seldom observed to contain more than two or three pili per cell. In contrast, nearly all cells of the nonprototype group B strain ATCC 13090 had numerous pili on their surfaces. When viewed in frozen-etched replicas, a few pili were observed lying on the cell surface of this latter strain. An annular structure was also found in frozen-etched replicas. This structure usually consisted of a series of concentric rings that were always found on the flat side of these bean-shaped cells. It is concluded that such structures represent a differentiated portion of the cell wall, which is involved in cross-wall formation during synthesis of the cell septum in dividing cells.


Ultraviolet light absorption and fluorescence properties of an allergen extracted from Ascaris suum.


Correlation between in vitro anaphylactic histamine release from tissues and reagin titer in rat.


Comparison of the chemical structure and biological activities of the glycolipids of Salmonella minnesota R595 and Salmonella typhimurium SL1102. It has been assumed in the past that the lipid moieties of endotoxic lipopolysaccharides are quite similar if not identical. This has been tested in the reported work here, where the chemical composition and biological activities of the glycolipids of two heptoseless Re mutants, Salmonella minnesota R595 and Salmonella typhimurium SL1102, have been studied and compared. The two glycolipids, extracted with chloroform-methanol (4:1), showed identical thin-layer chromatographic patterns. The molar ratios for hexosamine, fatty acids, phosphorus, 2-keto-3-deoxyoctonate, total amines, and total nitrogen of the purified glycolipids were in the same range, but small differences could be established. Both glycolipids contained the same major fatty acids, i.e., lauric, myristic, palmitic, and 3-hydroxymyristic acids, in similar but not identical ratios. On paper electrophoresis, the acid hydrolysates of the two glycolipids showed analogous components. In the determination of molecular weight, whereas S. minnesota R595 glycolipid did not show concentration dependence, the molecular weight measured for S. typhimurium SL1102 increased with its concentration. The molecular weight of the fully endotoxic R595 glycolipid has been found to be 17,000 +/- 1,500. Both glycolipids showed similar activities in the Shwartzman skin reaction, Limulus-lysate clotting assay, mouse lethality, and enhancement of nonspecific resistance, but the R595 preparation appeared to be more active on a weight basis in some parameters than SL1102. Using passive hemagglutination, we observed cross-reactivity between the glycolipids. In the gel-diffusion test, they revealed clear identity. The antiserum against S. minnesota R595 neutralized the Shwartzman skin reactivity not only of R595 but also of SL1102 glycolipid. These results confirm that there are identical immunodeterminant group(s) in the two glycolipids. On the other hand, chemical analytical data for two glycolipids showed only similarities, indicating that although both glycolipids are of comparable chemical nature, differences between them exist.


A search for markers of rabbit lymphoid cells; alloantigens characterized by antisera to rabbit thymus.


Gastric fetal sulphoglycoprotein antigen (FSA) and blood group antigens A and B.


Effect of divalent cations and metabolic energy on the anaphylactic histamine release from rat peritoneal mast cells.


In vitro studies of concanavalin-A-induced histamine release from human basophils: excess bridging in the inhibitory region of the dose-response curve.


Adjuvant activity for alkylamines of immune responses to ovalbumin, myelin basic protein and a tumor.


The inhibitory effect of nicotinamide on asthma-like symptoms and eosinophilia in guinea pigs, anaphylactic mast cell degranulation in mice, and histamine release from rat isolated peritoneal mast cells by compound 48-80.


Immune basophil degranulation: detection by iodonitrotetrazolium dehydrogenase stain.


Populations of guinea pig lymphocytes and Kurloff cells characterised by rosette-forming reactions.


ATP level and CO2 production of mast cells in anaphylaxis.


Studies on the cytotoxic immune reaction. IV. Occurrence and specificity of metabolically dependent cell surface antigens in various animal tissues.


Macro- and microdifferences in blood group antigens and antibodies. II. Subgroups of A, an example of graded microdifferences.


Interreceptoral junctions in the teleost retina.


Fine structure of Müller cells in the human retina as revealed by ruthenium red treatment.


Nitroblue-tetrazolium (NBT) reduction in leukocytes of children with acute leukemia.


Quantitation of DNA by cytofluorometry of the conventional Feulgen reaction.


The 'chess board' distribution of glycogen in liver. Artifact of fixation or the effect of an enzyme?