Purification and characteristics of pyocin B39 II. Physical and chemical properties. A purified preparation of pyocin B39 was shown to be homogeneous by disc electrophoresis and ultracentrifugation. Pyocin B39 is an antibacterial agent, protein in nature, and is capable of inhibiting the growth of susceptible cells. It caused a susceptible strain of Pseudomonas aeruginosa to release ultraviolet-absorbing material. Pyocin B39 appears to be a protein with a high molecular weight; its sedimentation coefficient is 32.8S. It was destroyed by 7 m urea but was resistant to attack by proteolytic enzymes, such as pepsin and trypsin. From its sedimentation coefficient, a molecular weight of about 2 x 10(6) was calculated. An electron photomicrograph of pyocin B39 revealed it as a rodlike particle, 7.50 pm long and 1.08 pm wide, with striations running at nearly right angles to the axes of the particle.


Antagonistic effect of calcium in serum on the activity of tobramycin against Pseudomonas. Physiological concentrations of calcium in serum antagonize the activities of colistin, polymyxin B, and gentamicin against Pseudomonas aeruginosa. Studies were carried out to determine whether tobramycin, a new aminoglycoside antibiotic, is also antagonized by calcium. The activity of tobramycin in vitro was shown to be antagonized by human serum and by physiological concentrations of calcium. The addition of human serum in broth-dilution tests produced a fourfold rise in the minimal inhibitory concentrations of tobramycin for five strains of Pseudomonas. In disc diffusion tests, the addition of calcium to the agar significantly decreased the size of inhibition zones, and the addition of a chelating agent to the agar increased the zone sizes. In a limited comparative study, tobramycin and gentamicin were tested against both light and heavy bacterial inocula of two strains of Pseudomonas. Tobramycin appeared to be antagonized less by serum than was gentamicin at equal antibiotic concentrations.


Enhanced toxicity for mice of vincristine and other chemotherapeutic agents with Salmonella typhosa endotoxin and Pseudomonas aeruginosa. The toxicity of Salmonella typhosa 0901 W endotoxin for BALB/c mice was potentiated by the administration of 375 mg of cyclophosphamide per kg, 16 mg of daunomycin per kg, 80 mg of methotrexate per kg, 8 mg of pactamycin per kg, 20 mg of polyinosinic-polycytidylic acid per kg, 1,000 mg of procarbazine per kg, and 1 or 4 mg of vincristine per kg. l-Asparaginase (20,000 units per kg) failed to potentiate endotoxin. Sedation following administration of 45 mg of pentobarbital per kg was prolonged in mice that had received 20,000 units of l-asparaginase per kg, 4 mg of daunomycin per kg, 120 mg of methotrexate per kg, 8 mg of pactamycin per kg, 10 mg of polyinosinic-polycytidylic acid per kg, 500 mg of procarbazine per kg, 2 mg of vincristine per kg, 2 mg of endotoxin per kg, or multiple doses of endotoxin. Mice pretreated with multiple endotoxin doses experienced a significant reduction in their lethal responses due to vincristine-endotoxin combinations; however, endotoxin-pretreated mice were more susceptible to vincristine alone than were normal mice. Simultaneous administration of 1 or 2 mg of vincristine per kg and 1 mg of endotoxin per kg produced greater lethality than sequential regimens. Pretreatment of mice with 65 mg of phenobarbital per kg on 4 consecutive days protected against vincristine-endotoxin combinations. Liver homogenates prepared from mice exposed previously to vincristine were capable of inactivating endotoxin. Vincristine lethality was increased by simultaneous administration of heat-killed cells of Pseudomonas aeruginosa isolated from mouse feces.


Effect of ethylenediaminetetraacetic acid and related chelating agents on whole cells of gram-negative bacteria. The effects of ethylenediaminetetraacetic acid and of four other chelating agents on seven strains of gram-negative bacteria are described. Changes in viability, cell lysis, and release of intracellular materials from suspensions in various buffers, pH 7.8 or 9.2, were observed. Cyclohexane-1,2-diaminotetraacetic acid was the most toxic compound, whereas iminodiacetic acid and nitrilotriacetic acid had little bactericidal activity. The activity of the five drugs appeared to be related to their chelating ability. Of the bacterial strains used, Pseudomonas aeruginosa NCTC 1999 and NCTC 6750 were the most susceptible, and Klebsiella aerogenes K1 was the most resistant. In contrast, the two strains of P. aeruginosa were the most resistant to the nonchelating antibacterial agents cetrimide, chlorhexidine, and benzalkonium chloride.


The aetiology, diagnosis and treatment of amoebic disease.


The effect of serotonin and prostaglandins on internal and external carotid blood-flow measured simultaneously in the monkey.


Functional and morphological observations on the effect of cyclophosphamide on the immune response of the chicken.


Origin of the various immunoglobulins in lymph from the popliteal lymph node of sheep before and after antigenic stimulation.


Prothrombin isolation and assay.

Prothrombin isolation and assay.


Proteolysis of rabbit immunoglobulin M by papain. 1. Digestion of rabbit immunoglobulin M (IgM) by papain for 5h, in the absence of reducing agent, gives Fabmu and Fc(5)mu fragments in high yield. Shorter periods give fragment Fc(5)mu with one or more Fabmu fragments still attached. 2. Reducing agent in the absence of a denaturant cleaves rabbit IgM into half-subunits, each containing one mu chain and one light chain. Digestion by papain in the presence of such a reducing agent destroys the Fcmu domains.


Membrane-bound DD-carboxypeptidases from Bacillus megaterium KM general properties, substrate specificity and sensitivity to penicillins, cephalosporins and peptide inhibitors of the activity at pH5. 1. The membrane from Bacillus megaterium KM contained a dd-carboxypeptidase with optimum activity under the following conditions: pH5.2, bivalent cation, 3mm; ionic strength, 40mm; temperature, 35 degrees C. It was inactivated by treatment with p-chloromercuribenzoate but was fairly insensitive to 2-mercaptoethanol. 2. The enzyme was inhibited by penicillins and cephalosporins. The inhibition of this enzyme was partially reversed on dialysis but 0.2m-2-mercaptoethanol could neither prevent nor reverse the inhibition. 3. The enzyme was extremely sensitive to changes in the configuration and size of the side chain of the C-terminal dipeptide of the substrate. An aliphatic side chain of a well-defined length and polarity was required in the residue that precedes the C-terminal dipeptide. 4. The enzyme was inhibited by a wide range of analogues of the peptidic portion of the natural substrate.


Kinetic studies on the effect of uridine diphosphate galactose and manganous ions on the reaction between lactose synthetase A protein from human milk and p-hydroxymercuribenzoate. The inhibition of lactose synthetase A protein by p-hydroxymercuribenzoate at pH7.5 and 25 degrees C, which involves the reaction of one molecule of inhibitor with each molecule of enzyme, was decreased in rate by UDP-galactose, especially in the presence of Mn(2+). Pseudo-first-order rate constants for the reaction between 0.1mm-p-hydroxymercuribenzoate and free enzyme, the enzyme-UDP-galactose complex and the enzyme-Mn(2+)-UDP-galactose complex were 4.4x10(-2), 1.9x10(-2) and 0.3x10(-2)min(-1) respectively. The results also indicated that dissociation constants for UDP-galactose in the enzyme-UDP-galactose and enzyme-Mn(2+)-UDP-galactose complexes were 313 and 16mum respectively, the latter value being similar to the K(m) for UDP-galactose in the lactose synthetase reaction. The protective effect of UDP-galactose and the role of Mn(2+) ions in lactose synthetase are discussed.


Biological, proton-magnetic-resonance- and ultraviolet-spectroscopic evidence for a molecular complex of actinomycin D and 10,11-dihydro-3H-naphth (1,2-g)indazol-7-ol. A proton-magnetic-resonance study of the complex formed between actinomycin D and 10,11-dihydro-3H-naphth[1,2-g]indazol-7-ol strongly suggests that the indazole lies above the phenoxazine ring of actinomycin D. The complex can be destroyed by addition of dimethyl sulphoxide or dimethylformamide. The actinomycin D-indazole complex inhibits growth of Pseudomonas fluorescens and raises the thermal denaturation response of DNA. These data support the hypothesis that a molecular complex is formed which readily inhibits cell growth and interacts with DNA.


Inhibition by progesterone of the lactogenic effect of prolactin in the pseudopregnant rabbit. The effects of progesterone on lactose synthesis activity and changes in mammary gland cells were studied in pseudopregnant rabbits simultaneously treated with prolactin.  The injection of progesterone alone on Days 15 and 17 of pseudopregnancy decreased the activity of lactose synthetase (LSA) and galactosyl transferase (GTA), while the administration of prolactin for 2-4 days increased their activities.  Th e simultaneous administration of progesterone and prolactin decreased the increase in LSA observed with prolactin alone by 70% on the 4th day of treatment, and decreased GTA by 30%.  Progesterone completely suppressed the polyribosome profile and the ratio of endoplasmic reticulum bound polyribosomes to free polyribosomes induced by prolactin.  The increase in RNA content in the mammary gland induced by prolactin was also suppressed by progesterone.  The results suggest that progesterone inhibits the lactogenic action of prolactin without interfe ring with its mammogenic role.


Soluble neutral and acid maltases in the suckling-rat intestine. The effect of cortisol and development. The 100000g supernatants from 13-day-old suckling-rat intestinal homogenates contained 43.5% of the total intestinal maltase activity, compared with 7.1% in weaned adult rats aged 40 days. The soluble maltase activity was separated on Sepharose 4B into two quantitatively equal fractions at pH6.0, one containing a maltase with a neutral pH optimum and the other a maltase with an acid pH optimum. The neutral maltase was shown to be a maltase-glucoamylase identical with membrane-bound maltase-glucoamylase in molecular weight, heat-sensitivity, substrate specificity, K(m) for maltose and K(i) for Tris. The soluble enzyme was induced by cortisol, but the ratio of the soluble to bound enzyme fell during induction. Solubility of the neutral maltase was not accounted for by the action of endogenous proteinases under the preparative conditions used. It is postulated that the soluble neutral maltase is a membrane-dissociated form of the bound enzyme and that the relationship between these two forms is modulated by cortisol. The acid maltase generally resembled acid maltase of liver, muscle and kidney. It was shown to be a maltase-glucoamylase with optimal activity at pH3.0, and molecular weight of 136000 by density-gradient centrifugation. At pH3.0 its K(m) for maltose was 1.5mm. It was inhibited by turanose (K(i)=7.5mm) and Tris (K(i)=5.5mm) but not by p-chloromercuribenzoate or EDTA. Some 55% of its activity was destroyed by heating at 50 degrees C for 10min. The acid maltase closely resembled beta-glucuronidase and acid beta-galactosidase in its distribution in the intestine, response to tissue homogenization in various media, and decrease in activity with cortisol treatment and weaning, indicating that it was a typical lysosomal enzyme concentrated in the ileum.


Water-soluble metabolites of p-nitrophenol and 1-naphthyl N-methylcarbamate in flies and grass grubs. Formation of glucose phosphate and phosphate conjugates. Metabolites isolated from houseflies dosed with 1-napththol or p-nitrophenol were identified as the phosphate and glucose phosphate conjugates of these phenols by titrations, hydrolysis, ionophoresis, i.r. spectra and mixed melting point. [(3)H]Carbaryl (1-naphthyl N-methylcarbamate) was metabolized by houseflies, blowflies and grass grubs to water-soluble metabolites which had chromatographic and ionophoretic behaviour similar to those of the conjugates of 1-naphthol with glucose, sulphate, phosphate and glucose 6-phosphate.


Protein synthesis and breakdown in the mother-cell and forespore compartments during spore morphogenesis in Bacillus megaterium. Recently developed techniques for isolating forespores from bacilli at all stages of spore morphogenesis have been exploited to investigate the contribution of each of the two compartments of the sporulating cell to the overall pattern of protein synthesis and degradation during sporulation in Bacillus megaterium. These studies have shown: (1) that protein synthesis continues in both compartments throughout spore morphogenesis; (2) that the degradation of proteins made at all times during vegetative growth and sporulation is confined to the mother-cell compartment; (3) that proteins synthesized in the mother-cell compartment during sporulation are subsequently degraded more rapidly than proteins synthesized during vegetative growth. This rate of degradation increases the later the proteins are synthesized in the sporulation sequence. Mature spores were disrupted, and the percentage of the total protein in soluble and particulate fractions was determined. Pulse-labelling experiments were performed to investigate the extent to which the proteins of these two fractions are newly synthesized during sporulation. These data were used to calculate the extent of capture of vegetative cell protein at the time of formation of the forespore septum. The value obtained is consistent with evidence from electron micrographs and supports a model for the origin of spore protein in which there is no protein turnover in the developing forespore.


The accumulation of malachite green stainable phospholipid in rabbit spermatozoa during maturation in the epididymis, and its possible role in capacitation.


Mass spectrometry of the methyl esthers of isomeric hydroxychlorobiphenyls--potential metabolites of chlorobiphenyls.


The characterization of a malachite green stainable, glutaraldehyde extractable phospholipid in rabbit spermatozoa.


A simple double immunodiffusion test for typing influenza viruses. The identification of influenza virus type has traditionally been based on the characterization of internal nucleoprotein (NP) antigens by the complement fixation (CF) test. Because this test is complex and time-consuming, it is used only infrequently. In this report we describe a double immunodiffusion (DID) test, which is proposed as a replacement for the CF test for the typing of influenza viruses. The DID test is simple and requires only the allantoic fluid content of a single infected embryonated egg. Virus from the infected fluid is precipitated with mild acid and disrupted by detergent. Anti-NP or anti-matrix protein (MP) antibody is equally effective in most instances for typing influenza isolates by this test. The latter is preferred, however, since it seemed to be slightly more sensitive than anti-NP antibody.


Quantitative measurement of precipitating antibodies and standardization of Streptococcus group B antisera by the reverse radial immunodiffusion technique. A comparative study was made of the reverse radial immunodiffusion (RRID) technique and the quantitative precipitin test for determining the amount of precipitable antibodies present in streptococcus group B antisera. The coefficient of correlation between the 2 tests was 0.963 when a purified carbohydrate antigen was used. The results of this study show that: (1) a group B antiserum should contain at least 3 mg of precipitable antibodies per ml to ensure that it will react with acid extracts of group B diagnostic cultures; and (2) the RRID technique is an accurate and simple procedure for determining the amount of precipitable antibody present in an antiserum, and it could be used for the standardization of these diagnostic reagents. A method for preparing the group B carbohydrate antigen used in the RRID and quantitative precipitin tests is described.


Prevalence of nonspecific tuberculin sensitivity in certain parts of India. A survey was carried out in certain parts of India to determine the prevalence of nonspecific tuberculin sensitivity. In the temperate zone, villages situated 1 200 m or more above mean sea level showed a markedly lower prevalence than villages in the plains did. In the tropical zone, the prevalence of nonspecific sensitivity was high. The prevalence of infection due to Mycobacterium tuberculosis was much lower than that of nonspecific sensitivity and the rates did not vary greatly in the areas surveyed.


Squamous metaplasia and respiratory tumors induced by intratracheal installations of 7, 12-dimethylbenz(a)-anthracene in Syrain golden hamsters.


A new pharmacological method of host conditioning in order to facilitate in vivo growth of fibroblast-like cells cultivated in vitro.


Observations on the organization of the interstitial tissue of the testis and on the occluding cell junctions in the seminiferous epithelium.


Prostaglandins and steroidogenesis.

Prostaglandins and steroidogenesis.


Blood group and HL-A specific substances in immune globulin and albumin preparations of human placental origin.


Freeze-dried virus antigen containing cells and FITC-conjugated immune sera for virus diagnostic purposes.


Concentration of normal immunoglobulins in human sera.


Isolation and control of human IgA and IgM preparations.


[Pathogenesis of aplastic anemia].

[Pathogenesis of aplastic anemia].


Interaction of concanavalin A with normal and leukemic lymphocytes: binding and agglutination.


Studies on double-stranded RNA synthesized in acute leukemia blast cells.


Congenital dyserythropoietic anemia (CDA), type I. Light and electron microscopy, erythroblastic proliferation, erythrokinetic and cytogenetic studies in one case.


Splenectomy in early chronic myeloid leukaemia: preliminary report on 37 cases.


Factor-XIII subunits-A and -S in congenital deficiency and in acute myeloblastic leukemia.


Virus-like particles in chronic lymphatic leukemia: ultrastructural findings.


A hematologist's appraisal of the problem cerebral lymphomas in transplanted patients. A speculative review.


[Frederick Stohlman Jr. 1926-1974].

[Frederick Stohlman Jr. 1926-1974].


Hemopoietic microenvironment in acute leukemia.


Conditions for purification of anti-Brucella antibodies by immunoadsorption and elution.


Further studies of human serum albumin in oral fluid.


Pyrimidine-sensitive drosophila wing mutants: withered (whd), tilt (tt) and dumpy (dp).


Organization of replicative units in salivary gland chromosome bands.


Gonococci and gonorrhea, I. Gonococci.

Gonococci and gonorrhea, I. Gonococci.


Host defenses in protein-calorie malnutrition.


Studies on the gastrulation of amphibian embryos: pseudopodia in the gastrula of Bufo bufo japonicus and their significance to gastrulation.


T and B lymphocytes.

T and B lymphocytes.


The microscopy of parotid atrophy after ligation of Stensen's duct.


Proteins of the periodontium. Biochemical studies on the collagen and noncollagenous proteins of human gingivae.


An intraoral model used for studies of fluoride incorporation in enamel.


The coccidial nature and life-cycle of Sarcocystis.


Laboratory studies on cefazolin--anti-inflammatory agent combinations with particular reference to tiaramide.


Providing a comprehensive district psychiatric service for the adult mentally ill. Appendix 3. Syndicate 'D' discussion paper.


With liberty and psychosis for all.

With liberty and psychosis for all.


Studies on the rate of diffusion-controlled reactions of enzymes. Spatial factor and force field factor.


The phosphate depletion syndrome: relation to caloric intake and phosphate infusion.


Prevention of ischemic myocardial contracture (stone heart).


Evolution of infarcts during reperfusion experiments in primates.


Species variability in clinical antihuman antithymocyte globulin production: rabbit vs horse AHTG in primate skin graft survival.


Cerebral etiology of the respiratory distress syndrome: universal response, with prevention by unilateral pulmonary denervation.


Trigeminothalamic projections from nucleus caudalis in primates.


Intravenous feeding in hepatic failure.

Intravenous feeding in hepatic failure.


Enhancement of peripheral nerve regeneration with triamcinolone after neurorrhaphy.


Modification of intestinal fistula output by elemental and intravenous alimentation.


Intravenous fat and glycerol: effect of heparin.


Reversible inhibition of phagocytosis in anephric uremic patients.


Hepatic glucose balance and glucose tolerance during hemorrhagic shock.


Postoperative positive protein balance: accelerated oxidative glucose consumption.


Study of copper metabolism in kinky hair disease (Menkes' disease) and in hepatolenticular degeneration (Wilson's disease) utilizing 67Cu and radioactivity counting in the total body and various tissues.


Mapping of functional pathways in brain by autoradiographic survey of local cerebral metabolism.


In search of the motor engram: a behavioral study of somatotopic localization in motor cortex of monkey.


Diagnosis and treatment of experimental allergic encephalomyelitis in rhesus monkeys.


Primate experiments on oral sensation and morphogenesis.


Sensation and morphogenesis experimental and clinical findings following functional forward displacement of the mandible.


Toxicity of Nerium oleander in the monkey (Cebus apella).


Penicillin and tetracycline sensitivity of Jamaican strains of gonococci.


Production of a rabbit anti-BKN antibody.


Endogenous prostaglandins and the initiation of labor. An attempt to define the role of the prostaglandins in human parturition must necessarily be fragmentary at the present time. However, there is no doubt that considerable progress has been made during the last few years and that the prostaglandins have been established as important key intermediates in the complex hormonal and mechanical events integrated in the process. Improved chemical methods for the determination of prostaglandins in body fluids and tissues as well as some evidence of indirect nature have contributed to a better understanding of this complex problem.


Effect of a diphosphonate on para-articular ossification after total hip replacement.


Amantadine.

Amantadine.


'Neuronal fall-out' in the ageing brain: a critical review of the quantitative data.


Phase III and health cost controls. No. L-89.


Skilled nursing - the issues and their impact.


DSCG and hyposensitization.

DSCG and hyposensitization.


Disodium cromoglycate, corticoids and bronchodilators in asthma therapy.


The chemotherapy of plasma cell myeloma and related disorders.


Granuloma formation and its relation to chemotaxis. A discussion of the role of two groups of micro-organisms: the mycobacteria and the anaerobic coryneforms.


Substances that attract eosinophils in vitro and in vivo, and that elicit blood eosinophilia.


Transport of intravenously administered horseradish peroxidase into the rat submaxillary saliva.


Adjuvant effects of oral microorganisms on the immunological responses.


Fractionation of nucleic acids from Penicillium chrysogenum and associated ribonucleic acid viruses by selective exclusion and retention in agarose gels. Double-stranded nucleic acids from a strain of Penicillium chrysogenum containing RNA viruses were isolated by agarose-gel filtration, and separated into DNA and double-stranded RNA fractions by agarose-gel chromatography in 2.5m-NaCl. The DNA fraction contained less than 1% alkali-labile polynucleotides, and sedimented homogeneously at 8-10S in alkaline sucrose gradients. In CsCl gradients it tended to band in the density region of 1.66-1.72g/ml. It had a ;melting' temperature (T(m)) of 75 degrees C in 0.015m-NaCl-0.0015m-trisodium citrate, corresponding to 51.5mol% of G+C. The double-stranded RNA fraction did not contain detectable DNA. It could not band in CsCl up to a density of 1.78g/ml, and mainly consisted of a 14-15S RNA species with a T(m) of 88.5 degrees C in the above solvent, and a G+C content of 49.3 mol%.


Affinity labelling of the binding site of rabbit antibody. Evidence for the involvement of the hypervariable regions of the heavy chain. The binding sites of rabbit antibodies with affinity for the haptenic group 4-azido-2-nitrophenyl-lysine have been specifically labelled by photolysis of the hapten-antibody complex. The extent of covalent labelling was 0.5-0.9mol of hapten bound/mol of antibody and, by using an immunoadsorbent, antibody with 1.3mol of hapten/mol was obtained. The antibody was specifically labelled in the binding site and the ratio of labelling of heavy and light chains was in the range 3.3-5.0. The labelled heavy chains were cleaved by CNBr treatment and after reduction and alkylation of the intrachain bonds, were digested with trypsin. Evidence is presented that two regions of the heavy chain, positions 29-34 and 95-114, together contain about 80% of the label on the heavy chain; these two regions respectively include two of the hypervariable regions of rabbit heavy chain.


The amine oxidases of human placenta and pregnancy plasma. 1. The purification of monoamine oxidase and diamine oxidase from normal human term placental tissue is described. 2. The properties of these enzymes are reported and compared with the properties of unpurified human pregnancy plasma. 3. This comparison shows that the amine oxidase of pregnancy plasma has properties corresponding to purified placental diamine oxidase, suggesting a placental origin for the plasma enzyme system. 4. Detailed kinetic study of the purified placental diamine oxidase suggests that it has a Ping Pong sequence, a mechanism of action and rate-limiting step similar to the diamine oxidase of pig kidney. 5. It is suggested that the enzyme system is important in protecting the foeto-placental unit from excesses of biogenic amines.


The inhibition of human placental diamine oxidase by substrate analogues. 1. The oxidation of p-dimethylaminomethylbenzylamine by purified placental diamine oxidase was followed by measuring the change in E(250) caused by the production of p-dimethylaminomethylbenzaldehyde. 2. The inhibition of this reaction by substrate analogues such as isothiouronium, guanidinium, dimethylsulphonium and trimethylammonium compounds was extensively studied. 3. The type and degree of inhibition by mono- and bis-onium compounds is described, and a theory is developed to explain the type of inhibition produced.


Stimulation of microsomal uridine diphosphate glucuronyltransferase by glucuronic acid derivatives. The glucuronic acid adducts of 1-naphthol, 2-naphthol and 4-methylumbelliferone activate microsomal UDP-glucuronyltransferase (EC 2.4.1.17) when the enzyme is assayed with p-nitrophenol as aglycone. Phenyl glucuronide and oestriol 3beta-glucuronide also activate UDP-glucuronyltransferase. but to a lesser extent. Activation by glucuronides is not dependent on metal ions, but is blocked by prior treatment of microsomal fractions with p-chloromercuribenzoate. The kinetic mechanism of activation is concluded to be an increase in the affinity of the enzyme for UDP-glucuronic acid. Activation by 1-naphthyl glucuronide, at high concentrations of p-nitrophenol, is not affected by 1-naphthol. Apparently 1-naphthyl glucuronide activates the preparation by binding at a site that is separate from the site of glucuronidation of 1-naphthol. Further evidence for the existence of distinct effector sites for the glucuronides was provided by the finding that activation by glucuronides is inhibited competitively by aglycone glucosides. These glucosides do not inhibit the rate of glucuronidation of p-nitrophenol in the absence of glucuronide adducts, nor do they alter the rate of glucuronidation of 1-naphthol. When UDP-glucuronyltransferase is assayed with 1-naphthol as aglycone it is activated by p-nitrophenyl glucuronide, 4-methyl-umbelliferyl glucuronide and under appropriate conditions by its own glucuronide. These activations are similarly inhibited by aglycone glucosides. p-Nitrophenyl glucuronide also stimulates the rate of glucuronidation of o-aminophenol, o-aminobenzoate and bilirubin.


Identical 3'-terminal octanucleotide sequence in 18S ribosomal ribonucleic acid from different eukaryotes. A proposed role for this sequence in the recognition of terminator codons. The 3'-terminal sequence of 18S ribosomal RNA from Drosophila melanogaster and Saccharomyces cerevisiae was determined by stepwise degradation from the 3'-terminus and labelling with [(3)H]isoniazid. The sequence G-A-U-C-A-U-U-A(OH) was found at the 3'-terminus of both 18S rRNA species. Less extensive data for 18S RNA from a number of other eukaryotes are consistent with the same 3'-terminal sequence, and an identical sequence has previously been reported for the 3'-end of rabbit reticulocyte 18S rRNA (Hunt, 1970). These results suggest that the base sequence in this region is strongly conserved and may be identical in all eukaryotes. As the 3'-terminal hexanucleotide is complementary to eukaryotic terminator codons we discuss the possibility that the 3'-end of 18S rRNA may have a direct base-pairing role in the termination of protein synthesis.


Studies on the 3'-terminal sequences of the large ribosomal ribonucleic acid of different eukaryotes and those associated with "hidden" breaks in heart-dissociable insects 26S ribonucleic acid. The 3'-terminal sequences associated with the large rRNA complex from a range of eukaryotes were determined after pancreatic or T(1)-ribonuclease digestion of RNA terminally labelled with [(3)H]isoniazid. In all higher eukaryotes examined except Drosophila melanogaster, the 3'-terminal sequences Y-G-U(OH) and G-C-U(OH) were demonstrated for the large RNA component(s) and for 6S RNA respectively. The 3'-terminal sequence of Saccharomyces cerevisiae 26S RNA was Y-G-U(OH) and that of 6S RNA Y-A-U-U-U(OH). Three 3'-terminal sequences were found in equimolar amounts in the heat-dissociable 26S rRNA characteristic of insect ribosomes. These were Y-G-U-G-U(OH), Y-C-G-U(OH) and G-C-U(OH) for cultured Antheraea eucalypti cells, Y-G-U(OH), Y-G-U(OH) and G-C-U(OH) for Galleria mellonella larvae and Y-C-G-A(OH), Y-G-U-A(OH) and G-Y-U-G(OH) for Drosophila melanogaster flies. Thus the introduction of the central scission in insect 26S rRNA results in the generation of a unique 3'-terminus and does not arise from random cleavage of the polynucleotide chain.


Mechanism of the mutagenic activity of diazoacetylglycine derivatives.


Problems peculiar to the diagnosis and management of asthma in children.


Cellular changes in intrauterine malnutrition.


Biochemical markers of intrauterine malnutrition.


Nitrogen balance during pregnancy.

Nitrogen balance during pregnancy.


Chorionic gonadotropin synthesized in cultivated trophoblast.


Chemical and immunological properties of iodocompounds associated with particulate fractions of hog thyroid cells.


Crystals in hyperfunctioning rat parathyroid cells.


The effects of hyperthermia on pregnancy and prenatal development.


Histopathological studies on liver tumorigenesis in rats treated with polychlorinated biphenyls.


Long-term cultures of leukocytes from leukemia and related disorders: methods and applications.


The X chromosome and the eye. The implications of recent microbiological models for clinical ophthalmology.


Melanizing activity of blood & semen of rabbits of different coat colours.


Salmonella para A cultured from S. mansoni surgically recovered from man.


Comparative infectivity for cattle of stabilates of Theileria lawrencei (Serengeti) derived from adult and nymphal ticks.


Immunoglobulin class of antibodies in monkeys infected with Toxocara canis.


Proteolytic activity of Pseudomonas aeruginosa on neopeptone agar medium.


Defense mechanisms in the mouth and their possible role in the prevention of dental caries: a review. The mouth harbors organisms in great abundance; yet their variety is limited and it is noteworthy that some species present in the mouth are not found elsewhere. There are many antibacterial substances in the mouth, some of which may contribute to the control of the microbial flora in the mouth. The activity of some is enhanced in the presence of specific antibody, e.g. lactoferrin, although others appear unaffected, e.g. lactoperoxidase. Opinions differ as to whether the level of immunoglobulins in human saliva can be related to caries. Although it has been established in principle that caries can be prevented in animals by means of vaccination, substantial work remains to be done before a vaccine will be available for testing in humans.


Keratin-filled pseudocysts of ducts of sebaceous glands in the vermilion border of the lip. Pathologic changes of free sebaceous glands are rare compared to those of the skin. Two keratin-filled pseudocysts which developed in the excretory ducts of sebaceous glands in the vermilion border of the lower lip in an 86-year-old woman are described. The entity is probably related to milia of the skin.


Protein synthesis in salivary glands of Drosophila melanogaster: relation to chromosome puffs.


The site of size constancy.

The site of size constancy.


Factors in the health care system undergoing change.


Whither virology? Trends and prospects in medical research.


Viral hepatitis and hepatitis B antigen: recent advances.


Directional, reversible currents of blood flow in spinal cord arteries.


Report on long term treatments with Sintisone (prednisolone-stearoylglycolate).


Anesthesiologic considerations in psychiatric convulsive therapy.


Violamycin, a new red-pigment antibiotic.


The cytopyge of ciliata. IV An experimental study of the ingestion, digestion and defaecation in Oxytricha fallax.


Occurrence and activity of ureolytic bacilli in soils.


Stability of 131I-thyroxine and of 131-Itri-iodothyronine: the influence of radiolytic disintegration of certain diagnostic tests.


Irradiation of small structures through the intact skull. The physical conditions for producing minute disc-shaped lesions for functional radiation surgery by means of narrow beam irradiation are discussed. The intention was a treatment procedure for routine clinical use and with high physical and mechanical reproducibility. The possibilities of using high energy electrons, supervoltage roentgen radiation, gamma emitting nuclides or fast neutrons in a technique for routine clinical use were investigated. The radiation of choice taking physical properties, radiation biologic factors and practical circumstances into account was considered to be 60-Co gamma radiation. A treatment apparatus containing 179 60-Co sources within a spherical sector of 70 degrees latitude and 160 degrees longitude was constructed.


Geriatric nursing practice. Relying on nurses to improve nursing homes. No. G-94.


Enzymes that repair or edit ultraviolet-irradiated DNA.


Effects of chemicals on light-induced responses in the isolated carp retina.


DNA repair in Bacillus subtilis: comparative studies with gamma-rays and ultraviolet light.


Origin and development of life in relation to solar energy.


Immunological cross reactions among strains of Hydrogenomonas, Pseudomonas and Alcaligenes.


Presence of growth factors for Methanobacterium ruminantium in both gram-positive and gram-negative bacteria.


Localization and phenotypic expression of a Tol marker in Citrobacter freundii.


The nature of quantitative genetic variation in drosophila. II. Average dominance of abdominal bristle polygenes.


The radioprotective effect of dimethyl sulfoxide on Bacillus subtilis cultures.


Translation of rabbit haemoglobin mRNA in oocytes of the Queensland cane toad, Bufo marinus.


A re-evaluation of energy-independent calcium-ion binding by rat liver mitochondria. The impermeability of the mitochondrial inner membrane to the chelator ethanedioxybis(ethylamine)tetra-acetic acid permits discrimination between Ca(2+) which has been transported to the internal (matrix) phase and Ca(2+) which binds to the external surfaces of the mitochondrion. With this technique, it is shown that ;energy-independent high-affinity' binding is a measure of carrier-mediated active Ca(2+) transport in respiration-inhibited mitochondria; the carrier also transports Ca(2+) to the internal phase after treatment with carbonyl cyanide m-chlorophenylhydrazone, but in this case the active-transport component is inhibited. The Ca(2+)-binding sites associated with the external membrane surfaces are similar in concentration and affinity for both inhibited and uncoupled mitochondria; it was not possible to measure external Ca(2+) binding which could be identified as carrier specific. The results are discussed in relation to the mechanism of mitochondrial Ca(2+) transport, and to previous studies of energy-independent Ca(2+) binding.


Separation, purification and properties of beta-lactamase I and beta-lactamase II from Bacillus cereus 569/H/9. 1. A procedure was devised which is suitable for the isolation of beta-lactamase I and beta-lactamase II from Bacillus cereus 569/H/9 on a large scale. After adsorption on to Celite both enzymes were eluted in good yield and separated by chromatography on Sephadex CM-50. 2. beta-Lactamase I was separated into three main components by isoelectric focusing and into two components by chromatography. 3. The Zn(2+)-requiring beta-lactamase II obtained by this procedure had a lower molecular weight (22000) than beta-lactamase I (28000) and also differed from the latter in containing one cysteine residue. 4. The beta-lactamase II contained no carbohydrate, but showed the thermostability of the enzyme isolated earlier as a protein-carbohydrate complex. 5. Amino acid analyses and tryptic-digest ;maps' indicate that some degree of homology between beta-lactamase I and beta-lactamase II is possible, but that beta-lactamase I is not composed of the entire sequence of beta-lactamase II together with an additional peptide fragment. 6. A 6-methylpenicillin and a 7-methylcephalosporin showed much lower affinities for both enzymes than did penicillins and cephalosporins themselves.


Metal cofactor requirements of beta-lactamase II. 1. The apoenzyme obtained on removal of Zn(2+) from beta-lactamase II from Bacillus cereus 569/H/9 showed less than 0.001% of the activity of the Zn(2+)-containing enzyme. 2. Removal of Zn(2+) led to a conformational change in the enzyme and partial unmasking of a thiol group. 3. Replacement of Zn(2+) by Co(2+), Cd(2+), Mn(2+) or Hg(2+) gave enzymes with significant, but lower, beta-lactamase activity. No activity was detected in the presence of Cu(2+), Ni(2+), Mg(2+) or Ca(2+). 4. Equilibrium dialysis indicated that the enzyme had at least two Zn(2+) binding sites. With benzylpenicillin as substrate the variation in activity with concentration of Zn(2+) indicated that activity paralleled binding of Zn(2+) to the site of highest affinity. 5. Replacement of Zn(2+) by Co(2+) and Cd(2+) gave enzymes with absorption bands at 340 and 245nm respectively, and raised the question of whether the thiol group in the enzyme is a metal-ion ligand. 6. Reduction of the product obtained by reaction of denatured beta-lactamase II with Ellman's reagent [5,5'-dithiobis-(2-nitrobenzoic acid)] gave a protein which could refold to produce beta-lactamase II activity in high yield.


Metabolic adaptations during lactogenesis. Lactose synthesis in rabbit mammary tissue during pregnancy and lactation. Lactose biosynthesis and relevant enzymatic activity in rabbit mamma ry tissue during various stages of pregnancy and lactation are investigated by using a tissue-slice incubation method in order to understand the temporal relationships.  Ovulation was induced in 27 New Zealand white rabbits and they were bred by artificial insemination.  Sacrifice occurred on days 15, 24, and 29 of pregnancy, and day 2, 5, 8, 15, and 22 post partum.  Nucleic acids were extracted and concentratons of DNA determined spectrophotometrically at 600 nm with diphenylamine reagent and RNA determined with orcinal reagent.  The tissue incubations were made with (U-14C) glucose.  (14C) lactose was then separated by paper chromatography from unchanged radioactive glucose.  Enzyme analysis including determining the activities of phosphoglucomutase, UDP-glucose pyrophosphorylase, and UDP-glucose 4-epimerase.  Lactose synthase was determined, as well as, hexokinase.  A biphasic adaptation in the rate of lactose synthesis and in the RNA concentration was noted during lactogenesis.  The 1st increase in the rate of lactose biosynthes is occurred between days 15 and 24 of pregnancy.  A 2nd substantial increase was noted immediately post partum.  The overall rate of lactose biosynthesis increased 12-fold from day 24 of pregnancy to day 15 of lactation post partum, and then decreased from 15 to 22 days post partum.  The RNA concentration/g wet weight of tissue and the ratio of RNA/DNA closely represented the biphasic ability of the mammary-tissue slice to synthesize lactose.  Increases in the activities of UDP-glucose 4-epimerase and lactose synthase were most closely correlated with increases in the rate of lactose biosynthesis.  UDP-glucose pyrophosphor ylase activity was unrelated with the ability to synthesize lactose, and hexokinase and phosphoglucomutase activities were variable during pregnancy and lactation.  Lactose synthase activity was present by day 15 of pregnancy, but the ability to synthesize lactose was undetected until day 24 of pregnancy.


The macromolecular properties of blood-group-specific glycoproteins. Characterization of a series of fractions obtained by solvent fractionation. 1. The glycoprotein components of a human ovarian-cyst fluid were isolated by a solvent [95% (w/w) phenol]-extraction procedure; the phenol-insoluble water-soluble glycoprotein was further fractionated by (NH(4))(2)SO(4) and by ethanol to yield eight fractions. 2. The fractions were analysed in terms of amino acids, fucose, galactose, N-acetylglucosamine, N-acetylgalactosamine and sialic acid. Variations occurred, particularly in the proportion of peptide; these were partly correlated with varying extent of serological activity. 3. The fractions were characterized physicochemically in terms of buoyant density and degree of spreading in a density gradient, sedimentation velocity and molecular weight; their partial specific volumes and specific refraction increments were also determined. 4. The fractions showed wide variations in their sedimentation-velocity and density-gradient patterns, and gave evidence of pauci-dispersity in density. The fraction regarded as the most typical blood-group-specific glycoprotein sedimented as a single rapidly spreading peak and was of high molecular weight. 5. Significant correlations were observed between the physical properties of the glycoprotein fractions and the amount of their peptide component. The buoyant densities and sedimentation coefficients varied in a manner that suggested the existence of two families of glycoproteins. 6. It is suggested that variability in the extent of glycosylation, or in the degree of cross-linking, might account for the two families of glycoproteins, and that the extent of cross-linkage might also be a factor determining the solubility of these glycoproteins in hot saturated (NH(4))(2)SO(4).


The amino acid sequence of the peptide containing the thiol group of creatine kinase from normal and dystrophic chicken breast muscle. Comparison of some of the immunological properties of the antibodies developed in rabbits against these enzymes. The major (14)C-labelled peptides from creatine kinase from normal and dystrophic chicken muscle obtained by carboxymethylating the reactive thiol groups with iodo[2-(14)C]acetic acid and digestion with trypsin were purified by ion-exchange chromatography on Dowex-50 (X2) and by paper electrophoresis. The chromatographic characteristics of the (14)C-labelled peptides, their electrophoretic mobilities at pH6.5, and their amino acid compositions were identical for the two enzymes. The sequence of amino acids around the essential thiol groups of creatine kinase from normal and dystrophic chicken muscle was shown to be Ile-Leu-Thr-CmCys-Pro-Ser-Asn-Leu-Gly-Thr-Gly-Leu-Arg (CmCys, carboxymethylcysteine). This sequence is almost identical with that for the creatine kinases in human and ox muscle and bovine brain and is very similar to that of arginine kinase from lobster muscle. Antibodies to the enzymes were raised in rabbits and their reaction with the creatine kinase from normal and dystrophic muscles in interfacial, immunodiffusion and immunoelectrophoretic experiments was studied. The cross-reaction between normal muscle creatine kinase and antisera against the dystrophic muscle enzyme (or vice versa) observed by immunodiffusion and by immunoelectrophoretic experiments further suggests that the enzymes from normal and dystrophic chicken muscle are similar in structure. The results of the present study, the identical amino acid sequence of the peptides containing the reactive thiol group from both the normal and dystrophic chicken muscle enzymes and the immunological similarities of the two enzymes are in accord with the similarity of the two enzymes observed by Roy et al. (1970).


Polyribosomal and particulate distribution of lysyl- and phenylalanyl-transfer ribonucleic acid synthetases. 1. Only two aminoacyl-tRNA synthetases from Chinese hamster ovary cells are found associated with ribosomes and polyribosomes. 2. Phenylalanyl-tRNA synthetase activity is found with the 60S subunit, 80S monoribosome and individual polyribosomes. An additional 15S form of the enzyme is also seen. 3. Lysyl-tRNA synthetase activity is found in a form of about 20S and associated with ribosomal subunits and polyribosomes. The ribosomal subunits having lysyl-tRNA synthetase activity are about 6S larger than the bulk of the ribosomal subunits. 4. The lysyl- and phenylalanyl-tRNA synthetases found in different complexes have differential sensitivity to EDTA and centrifugation properties.


Preliminary characterization of Thy-1.1 and Ag-B antigens from rat tissues solubilized in detergents. 1. A radioactive binding assay for Thy-1.1 alloantigen which functions in the presence of detergents was established by using glutaraldehyde-fixed thymocytes as target cells. Thy-1.1 activity in detergent extracts was then assayed by measuring inhibition of the binding assay. 2. Solubilization of Thy-1.1 from whole thymocytes, and their membranes by a large number of non-ionic detergents and deoxycholate was studied. In the same extracts Ag-B(4) histocompatibility antigenic activities were measured. With the exception of Nonidet P-40, the detergents did not affect the antigenicity of Thy-1.1, but only Lubrol-PX and deoxycholate gave effective solubilization as measured by activity remaining in the supernatant after centrifugation at 200000g for 40min. With Ag-B(4) antigen, Triton X-100, Triton X-67 and Nonidet P-40 gave effective solubilization as well as Lubrol-PX and deoxycholate. Solubilization of Thy-1.1 activity from leukaemia cells and a brain homogenate was also studied, but none of the non-ionic detergents gave satisfactory results with these tissues. 3. Extracts from thymocyte membranes were further examined by gel filtration and sucrose gradient centrifugation. The Thy-1.1 activity behaved as a single component in deoxycholate with a density similar to that of a globular protein, but in Lubrol-PX the antigen was contained in a low-density complex. In Lubrol-PX extracts Ag-B(4) was also found in aggregates not observed in deoxycholate. 4. The s(20,w) values for Thy-1.1 and Ag-B(4) antigens in deoxycholate were 2.4 and 4.4, and v values were 0.70 and 0.75 respectively. The Stokes radius observed for Thy-1.1 was 3.1nm and for Ag-B(4) 5.3nm. By using these values the molecular weights for the antigen-detergent complexes were calculated to be 28000 for Thy-1.1 and 100000 for Ag-B(4).


The localization of a vitamin K-induced modification in an N-terminal fragment of human prothrombin. 1. The N-terminal fragment (PF-I) split off from prothrombin during coagulation was purified to homogeneity from human serum. 2. The apparent molecular weight is 27000+/-2000 in sodium dodecyl sulphate-polyacrylamide-gel electrophoresis, whereas a value of about 19600 is obtained by calculation based on amino acid and carbohydrate analyses. The N-terminal sequence is an Ala-Asx bond. The fragment contains about 16% carbohydrate, binds phospholipids in the presence of Ca(2+) and is adsorbed to BaSO(4). The pK(a) of its BaSO(4)-binding group(s) is 3.1-3.5. 3. By CNBr cleavage of fragment PF-I two peptides (C-1 and C-2) were obtained with molecular weights of about 5900 (C-2) and 12400 (C-1) on the basis of amino acid and carbohydrate analyses. Only the smaller (N-terminal) peptide is adsorbed to BaSO(4) and, since the ability of the whole protein to bind to BaSO(4) is known to be absent in samples obtained from patients treated with vitamin K antagonists, this peptide probably contains the site of a modification to the structure of the protein which occurs during biosynthesis and depends on vitamin K. This peptide does not contain hexosamine or sialic acid.


An evaluation of an agar gel diffusion test with crude and purified antigens in the diagnosis of hydatid disease. The sensitivity of the indirect haemagglutination test for the diagnosis of hydatid disease, although high, is insufficient. Agar gel diffusion tests for the diagnosis of this disease have not received much attention in the past and have been considered unsatisfactory. The authors propose such a test and evaluate its results in comparison with those of the indirect haemagglutination test.


Seroepidemiology of Congo virus (related to the virus of Crimean haemorrhagic fever) in Nigeria. A survey for neutralizing antibodies to Congo virus was carried out in Nigeria. Of a total sample population of 250, comprising 141 males and 109 females, 9 males and 15 females had antibody levels >/= 1.5 log neutralization index. In the age group 0-10 years 4 of 84 males and 14 of 79 females had antibody levels >/= 1.5 log neutralization index. There was no evidence of nonspecific antiviral activity in the sera tested.


The ovicidal effect of selected chemicals against eggs of Echinococcus granulosus. The ovicidal activity of various chemicals against eggs of E. granulosus was evaluated by measuring infectivity to CF(1) mice after exposing the eggs to aqueous solutions of the chemicals for 1 h at room temperature. Significant reductions in infectivity were observed in eggs treated with Lugol's iodine, 0.015% ammonium dodecylbenzenesulfonate, 70% ethanol, or 5% or 10% glutaraldehyde. No cysts developed in 10 mice that received doses of 1 000 eggs treated with 5% glutaraldehyde, and only 1 of 10 mice developed cysts after exposure to eggs treated with 10% glutaraldehyde.


Antitumor activity of cell-wall skeletons and peptidoglycolipids of mycobacteria and related microörganisms in mice and rabbits.


Organophosphorus poisoning.

Organophosphorus poisoning.


Some genetic interrelationships in England and Wales. A methodological investigation.


[Diagnosis, epidemiology and prophylaxis of equine infectious anaemia (author's transl)].


Some epidemiologic studies of transfusion-associated hepatitis.


Circadian rhythm in Bence-Jones protein excretion by LOU rats bearing a transplantable immunocytoma, responsive to adrimycin treatment.


A brief discussion of a model for improving the cost effectiveness of a token economy in a rehabilitation setting.


Experimental endocarditis.

Experimental endocarditis.


Pseudomonas aeruginosa serotypes encountered in the American University Hospital (AUH), Beirut, Lebanon.


Postnatal undernutrition and human brain development.


Vasospasm and adrenergic innervation of circle of Willis. Cerebral vasospasm following the rupture of intracranial aneurysm presents perplexing situation, i.e. prolonged vasospasm results in cerebral infarct and too early relief causes recurrence of the bleeding. To understand mechanism underlying the cerebral vasospasm, catecholamine-containing sympathetic nerve plexus was examined in the arterial walls of the circle of Willis using rat, cat, dog and monkey using Falck-Hillarp's fluorescence method. The distribution of fluorescing nerve plexus was found to be essentially the same in the examination of all species. The distribution of the fluorescing fibers decreases in the following order; proximal anterior cerebral, internal carotid, distal anterior or proximal middle cerebral, and distal middle cerebral artery. The origin of the nerve plexus accompanying the basal cerebral arteries and the relation to the experimental cerebral vasospasm was investigated as detailed as they could.


Islet cell transplantation.

Islet cell transplantation.


End-stage diabetic nephropathy. Discussion: general topics and summation.


A computer-aided diagnostic system using a small desk-top computer-calculator.


An accurate method for measuring immunodiffusion rings.


Infusion of synthetic TRH in the diagnosis of thyroid and pituitary dysfunction.


Comparison of short wave diathermy and pulsed electromagnetic energy in treatment of soft tissue injuries.


Technique for the visualization of precipitation bands obtained by gel immunodiffusion reactions for hepatitis B antigen detection.


Clinical evaluation of Morfolep.

Clinical evaluation of Morfolep.


Angina pectoris and nitroglycerin.

Angina pectoris and nitroglycerin.


Split and extremely thin glomerular basement membranes in hereditary nephropathy (Alport's syndrome).


The mode of growth of experimental metastases in rabbit femora.


Aorta valve replacement using autologous fascia lata transplants. Late morphological changes, an electron microscopic study.


Electron microscopy of thrombocytes in the orthotopic porcine liver homograft during the late rejection. (Phagocytosis of thrombocytes by Kupffer cells).


Hypothalamic neurosecretory nuclei and nucleus habenularis of epithalamus in essential hypertension.


Liver schistosomiasis and primary biliary cirrhosis. A clinico-pathological study.


Laboratoty techniques in rabies: tissue culture methods.


Laboratory techniques in rabies: the complement fixation test.


Safety precautions in handling rabies virus.


Laboratory techniques in rabies: hamgglutination and hemagglutination-inhibition tests.


Passive haemagglutination test for rabies antibodies.


Laboratory techniques in rabies: gel-diffusion techniques.


Laboratory techniques in rabies: electron microscopy.


Purification of rabies virus and isolation of its components.


Aluminium phosphate method for rabies virus purification.


A radioimmune assay for rabies binding antibody.


Laboratory technics in rabies: general considerations in vaccine production.


An assessment of laboratory techniques in the diagnosis and prevention of rabies and in rabies research.


Laboratory technics in rabies: Semple-type vaccine.


Laboratory techniques in rabies: Fermi-type vaccine.


Laboratory techniques in rabies: phenolized, freeze-dried sheep brain vaccine. A. Method used in the USSR.


Laboratory techniques in rabies: phenolized, freeze-dried sheep brain vaccine. B. Method used at the Pasteur Institute, Paris.


Laboratory techniques in rabies: suckling rat brain vaccine.


Laboratory techniques in rabies: ultraviolet-light irradiation for inactivation of vaccines.


Laboratory techniques in rabies: suckling rabbit brain vaccine.


Laboratory techniques in rabies: suckling mouse brain vaccine.


Laboratory techniques in rabies: chicken-embryo vaccine.


Laboratory techniques in rabies: tissue culture vaccines for animals.


Laboratory techniques in rabies: duck-embryo vaccine.


Laboratory techniques in rabies: vaccine for man prepared in human diploid cells.


Laboratory techniques in rabies: general considerations in safety and potency testing.


Laboratory techniques in rabies: Habel test for potency.


Laboratory techniques in rabies: modified Habel test for potency.


Laboratory techniques in rabies: the NIH test for potency.


Laboratory techniques in rabies: guineapig potency test for chicken-embryo vaccine.


Laboratory techniques in rabies: shipments of specimens, and techniques for preparation of animal tissues.


Laboratory techniques in rabies: test for neuroallergenic activity.


Laboratory techniques in rabies: the antibody binding test for vaccine potency.


Production of antirabies serum of animal origin.


Preparation of antirabies immunoglobulin of animal origin: method used in the USSR.


Preparation of antirabies immunoglobulin of human origin.


Quantitative assay and potency test of antirabies serum and immunoglobulin.


Laboratory techniques in rabies: methods of calculation.


A rapid method for fluorescein labelling of rabies antibodies.


Laboratory techniques in rabies: cell monolayer plaque test.


Proposed safety tests for inactivated rabies vaccine prepared in cell cultures.


A rapid tissue culture test for determining rabies neutralizing antibody.


The immunoperoxidase reaction for demonstration of rabies virus.


Laboratory techniques in rabies: the fluorescent antibody test.


Laboratory techniques in rabies: rapid microscopic examination for Negri bodies and preparation of specimens for biological test.


Laboratory techniques in rabies: the mouse inoculation test.


Laboratory techniques in rabies: the virus neutralization index test in mice.


Fixation with mercury salts.

Fixation with mercury salts.


[Two simple but useful methods for the analysis of dyes: gel-filtration chromatography and solvent extraction].


The importance and histopathology of hypothalamic changes in animals inoculated intracerebrally with Högyes' fixed strain of rabies virus.


The choriocapillaris.

The choriocapillaris.


Submacular choroidal vascular pattern. Experimental fluorescein fundus angiographic studies.


The long posterior ciliary arteries. An experimental study.


Temperature-induced change in helicity of the inherently dissymmetrical disulfide group in cyclocystine.


The problem of species variations.

The problem of species variations.


Pleiotropic mutations affecting sporulation conditions and the syntheses of extracellular enzymes in Bacillus subtilis 168.


Identification of the structural gene for sucrase in Bacillus subtilis Marburg.


[Antibacterial activity in vitro of cyclacillin (author's transl)].


The isozymes of phosphorylase kinase in various mammalian tissues.


Cranial bony changes in ocular neurofibromatosis.


A rare association of neurofibromatosis with Marfan's syndrome.


Antibiotics and war injuries.

Antibiotics and war injuries.


In vitro studies linking induction of spontaneous antibody synthesis to an accessory cell with persisting antigen on its surface.


The value of serological techniques for the differentiation between Verticillum Albo-atrum Reink. & Berth. and V. dahliae Kleb.


A comparison of autoimmunity and experimental allergic thyroiditis in strain 2 and Hartley strain guinea pigs.


Serological studies on coffee rust.

Serological studies on coffee rust.


Effects of pretreatment of mice with BCG cell walls in saline on subsequent vaccination with BCG oil droplet vaccine.


Sterilization of Listeria monocytogenes by guinea pig peritoneal exudate cell cultures.


Quantitative analysis of the 51Cr release cytotoxicity assay for cytotoxic lymphocytes.


Exclusion of cooperating T cells as targets for heterologous anti-mu antiserum.


Immunological memory in tuberculosis. I. Influence of persisting viable organisms.


Immunosuppression of congenitally athymic (nude) mice with heterologous anti-immunoglobulin heavy-chain antisera.


Ontogeny of B lymphocytes. I. In vitro appearance of Ig-bearing lymphocytes.


Development of lymphocyte populations in the human foetal thymus and spleen.


B-cell markers on lymphoblasts in acute lymphoblastic leukaemia.


Monoclonal lymphocyte population in human plasma cell myeloma.


Fine structural alterations in the developing spermatids of Musca domestica induced by the PCB, Aroclor 1254.


Androgens in prenatal development: behavior changes in nonhuman primates and men.


Thyrotropin releasing hormone induced thyrotropin release in euthyroid men. Effect of ethinyl oestradiol priming.


Intravenous alimentation with a mixture of fat, carbohydrates and amino acids in small immature newborn infants--a preliminary report.


Proceedings: Neuron activities in prefrontal cortex during gazing behavior in monkeys.


Proceedings: Prefrontal activities in monkeys inmaturely trained in delayed response task.


Proceedings: Neuron activities in the posterior parietal association areas and visual orientation.


Proceedings: Activities of motor cortex neurons during simple reaction time responses in monkeys.


Proceedings: Plasma levels of luteinizing hormone during menstrual cycle and their response to gonadectomy in Japanese monkeys.


Editorial: Suppression of Rh-sensitisation by the use of anti-D immunoglobulin.


Genetic code limit organisms--do they exist?


Characterization of the primary structural homology between the 16s ribosomal RNAs of Escherichia coli and Bacillus megaterium by oligomer cataloging.


A comparison of betamethasone valerate aerosol and sodium cromoglycate in children with reversible airways obstruction.


The effect of betamethasone valerate aerosol in exercise-induced asthma.


Haemodynamics in animals.

Haemodynamics in animals.


Psychotherapy research in schizophrenia--another view of present reality.


Patterns of psychiatric utilization by diagnosed schizophrenics in the Kansas City area.


Studies on interaction of 4-nitroquinoline 1-oxide with RNA of hamster embryo cells.


Electron microscopic study of plasma cells in immunoproliferative diseases. Preliminary report.


Problems of macromolecule ultrafiltration.


Perspectives offered by enzyme binding in polymeric membranes.


Extracapillary proliferative glomerulonephritis so-called malignant glomerulonephritis.


Unilateral renovascular hypertension in the dog.


Pharmacology of diuretics: a progress report, 1968--1971.


Diuretics in chronic renal insufficiency.


Metabolic complications in the use of diuretics.


Diuretics in the treatment of arterial hypertension.


Biochemical studies of the glomerular basement membrane in the diseased kidney.


Trapping of urea and ammonia in the gut of uremic patients.


Biochemical studies of the glomerular basement membrane in the normal kidney.


Pathogenesis of the uremic state: a new perspective.


The structural basis of glomerular filtration.


Artificial membranes.

Artificial membranes.


Role of anti-basement membrane immunity in human glomerulonephritis.


Glomerular filtration of macromolecules.


Interface reactions between artificial membranes and blood.


Cryoglobulinemia in glomerulonephritis exclusive of general diseases.


Ultrastructural lesions of the glomerular basement membrane in hereditary chronic nephritis.


Intravascular coagulation and nephropathies.


Acute renal failure and intravascular coagulation.


Renal disease and fibrinogen degradation products.


Home dialysis in the United Kingdom: experience in a regional center.


Maintenance home dialysis: four years' experience in Languedoc-Roussillon.


One hundred patients on home dialysis.

One hundred patients on home dialysis.


Drugs in the treatment of hyperuricemia.


The Lesch-Nyhan syndrome.

The Lesch-Nyhan syndrome.


Recent data on uric acid lithiasis.

Recent data on uric acid lithiasis.


Hyperuricemia in children, with the exception of the Lesch-Nyhan syndrome.


Ureteral diverticulosis. A new case and a review of the literature.


Ultrastructural concepts of drug metabolism. I. The hepatocyte: structure and function.


Ultrastructural concepts of drug metabolism. II. The hepatocyte: phenobarbital and microsomal enzyme induction.


A comprehensive approach to the treatment of drug dependence.


Ultrastructural concepts of drug metabolism. III. The hepatocyte: membranes, lipids, and alcohol.


The detection and differentiation of the products of the human carbonic anhydrase loci, CAI and CAII using fluorogenic substrates.


Further studies on erythrocyte inorganic pyrophosphatase: an examination of different mammalian species and human-Chinese hamster hybrid cells.


Glycoprotein biosynthesis in splenic cells. IV. Subcellular localization of glycosyl - transferases.


The economics of health.

The economics of health.


A clinical and sociomedical study for defaulting tuberculosis treatment.


Development of functional connexions in the mammalian visual system.


Selection of visual targets for the initiation of saccadic eye movements.


Single unit firing patterns in the vestibular nuclei related to voluntary eye movements and passive body rotation in conscious monkeys.


The role of vestibular and neck afferents during eye-head coordination in the monkey.


Development of sensorially-guided reaching in infant monkeys.


Movement without proprioception.

Movement without proprioception.


Some examples of programmed limb movements.


The activity of dentate neurons during an arm movement sequence.


Relation of basal ganglia, cerebellum, and motor cortex units to ramp and ballistic limb movements.


Single unit recording in the caudate nucleus during sessions with elaborate movements in the awake monkey.


Pyramidal tract discharge in relation to movement performance in monkeys with partial anaesthesia of the movind hand.


Role of occipito-frontal cortico-cortical connections in visual guidance of relatively independent hand and finger movements in rhesus monkeys.


Gating of motor cortex reflexes by prior instruction.


Cortical load compensation during voluntary elbow movements.


Short review. Prospects for cancer immunochemotherapy.


Structure-activity correlations for N-substituted N-thioformylhydroxylamines on bacterial cells.


Formation of the red neotetrazolium formazan by reduced nicotinamide adenine dinucleotide phosphate-cytochrome c reductase in the presence of triton X-100.


Studies on dextranase. V. Activation of dextranase from Penicillium funiculosum IAM 7013 by Co2+.


Experiments with cholera toxin detoxified with glutaraldehyde. Studies on the production, purification, and detoxification of cholera toxin are reported. The toxin was first partially purified with aluminium hydroxide and further purification was effected with Bio Gel A-5m and Sephadex G-75. The toxins were detoxified with glutaraldehyde; the toxoids so obtained, when injected into the skin of rabbits, appeared to have no residual toxicity. All the toxins were immunogenic. The toxoid partially purified with aluminium hydroxide retained most of its immunizing capacity, but the highly purified toxoids did not retain their capacity to stimulate antibody production in rabbits.


Experiments on monkeys with cholera toxin partially purified and detoxified with formol and glycine. Cholera toxoid partially purified and detoxified with formol and glycine and inoculated in monkeys proved safe. Histological examination revealed no changes in the skin of the animals treated. Blood samples taken from monkeys immunized with 2 doses of toxoid 12 days after the second inoculation revealed appreciable levels of antitoxin in the animals that had received two 100-mug doses of toxoid. The monkeys vaccinated with toxoid, when inoculated with challenge doses of cholera toxin, had the capacity to neutralize the toxin. Detoxification with formol and glycine may be the first step towards the preparation of a highly purified, innocuous, and antigenic cholera toxoid.


Immunology of schistosomiasis.

Immunology of schistosomiasis. This Memorandum, after summarizing the life cycle of the different species of human schistosome, reviews the present knowledge of the immunology of schistosomiasis. Each stage of the parasite contains antigen that may stimulate an immune response. However, at the moment there are no accepted serological in vitro tests that correlate with protection; this develops only after the host has experienced a living infection, which suggests that the stimulation of immunity is due to some metabolic process involving the release of protective antigen. The adult worm, however, seems to be able to escape the immune mechanism of the host. Specific antigens are also released by the eggs, and the immune response against these antigens seems to cause granuloma formation around the egg itself. The granuloma is the main lesion found in schistosomiasis. Evidence for protective immunity in experimental animals and man is reviewed, together with the possible mechanism by which the adult worm escapes the immune response of the host. A review of methods used for the diagnosis of schistosomiasis and a list of recommendations for further research are also included.


The natural history of the antibody response to breast antigens.


Immunological competence in non-human primates: differences observed in four species.


Surface immunoglobulins in chronic lymphatic leukaemia, macroglobulinaemia and myelomatosis.


Properdin levels in systemic lupus erythematosus and membranoproleferative glomerulonephritis.


Rosette formation by mouse lymphocytes. II. T-cell specificities in a CRL subpopulation.


Adhesion of human blood platelets to glass and polymer surfaces. I. Studies with platelets in plasma.


In vitro granulocyte-platelet rosette formation mediated by an IgG immunoglobulin.


Biochemical foundations of preventive medicine: the study of abnormal enzymes.


Need for long-term care of the inhabitants of Helsinki city.


Beta-lactam antibiotics. I. Comparative structure--activity relationships of 6-acylaminopenicillanic acid derivatives and their 6-(D-alpha-acylaminophenylacetamido) penicillanic acid analogues.


Therapeutic efficacy of bicyclomycin for shigellosis experimentally induced in rhesus monkeys.


Bacteriological studies on the combined action of aminoglycoside antibiotics and synthetic penicillins against Pseudomonas aeruginosa.


Ultrasound localization of the kidneys for closed renal biopsy.


The ultrasonic determination of renal transplant volume.


The ultrasound findings in an obstructed afferent loop.


The diagnosis of sextuplets by ultrasound-a case report.


The effect of phenylalanine, glycine, lysine, thymidine and thalidomide upon the developmental age in Drosophila melanogaster.


Immunoelectrophoretic analysis of surface antigens in vegetative cells and spores of Bacillus cereus.


Ultrasound diagnosis of and cyst puncture guidance for a second unilateral renal cyst.


A simple technique to determine the extent of abdominal aortic aneurysms of B-mode sonography.


Renal pelvic lipomatosis: a sonographic evaluation.


The gray scale echographic appearance of thyroid malignancy.


B-mode ultrasonic examination of the testes.


Ultrasonic evaluation of postpartum sepsis with retained Dalkon shield.


Simple device for the calibration of ultrasonic calipers.


Acoustic microscopy of mammalian kidney.


Pitfalls in the echographic evaluation of pancreatic disease.


Gray scale ultrasonography in the diagnosis of thyroid swellings.


Comparison of pelvic ultrasonography and pneumography for ovarian size.


The inhibitory action of diazoxide on uterine activity in the subhuman primate: Placental transfer and effect on the fetus.


The case against ankle taping.

The case against ankle taping.


Failure of dialysis concentrate as a bactericidal agent.


Enzyme therapy in Fabry's disease by renal transplantation.


The ankle: soft tissue injuries.

The ankle: soft tissue injuries.


Ankle injuries in sports.

Ankle injuries in sports.


A new subcutaneous prosthetic A-V shunt: advantageous over the standard Quinton-Scribner shunt and A-V fistula.


The swivel football shoe: a controlled study.


Prediction of long term combined imuran ALG dosage.


New long-stay inpatients.

New long-stay inpatients.


New long-stay patients.

New long-stay patients.


The hospital census as a sampling frame for studies of long-term psychiatric care.


Influence of nitroglycerin on the isolated blood-perfused dog heart.


Effect of calcium ions on the optical rotation of the troponin complex, troponin A and troponin B.


Purification and properties of 3-hexulose phosphate synthase and phospho-3-hexuloisomerase from Methylococcus capsulatus. 3-Hexulose phosphate synthase and phospho-3-hexuloisomerase were purified 40- and 150-fold respectively from methane-grown Methylococcus capsulatus. The molecular weights of the enzymes were approximately 310000 and 67000 respectively, as determined by gel filtration. Dissociation of 3-hexulose phosphate synthase into subunits of molecular weight approx. 49000 under conditions of low pH or low ionic strength was observed. Within the range of compounds tested, 3-hexulose phosphate synthase is specific for formaldehyde and d-ribulose 5-phosphate (forward reaction) and d-arabino-3-hexulose 6-phosphate (reverse reaction), and phospho-3-hexuloisomerase is specific for d-arabino-3-hexulose 6-phosphate (forward reaction) and d-fructose 6-phosphate (reverse reaction). A bivalent cation is essential for activity and stability of 3-hexulose phosphate synthase; phospho-3-hexuloisomerase is inhibited by many bivalent cations. The pH optima of the two enzymes are 7.0 and 8.3 respectively and the equilibrium constants are 4.0x10(-5)m and 1.9x10(2)m respectively. The apparent Michaelis constants for 3-hexulose phosphate synthase are: d-ribulose 5-phosphate, 8.3x10(-5)m; formaldehyde, 4.9x10(-4)m; d-arabino-3-hexulose 6-phosphate, 7.5x10(-5)m. The apparent Michaelis constants for phospho-3-hexuloisomerase are: d-arabino-3-hexulose 6-phosphate, 1.0x10(-4)m; d-fructose 6-phosphate, 1.1x10(-3)m.


Nature of the antigen present in the cerebrospinal fluid and serum of patients with group A meningococcal meningitis.


Specific antisera to Clr. Quantitation of Clr in normal and pathological human sera.


Sequential cell-mediated immune responses in experimental gingivitis in man.


Inactivation of C1 in rheumatoid synovial fluid, purfied C1 and C1 esterase, by gold compounds.


Analytical study of salivary immunoglobulins in multiple myeloma.


PHA-induced lymphocyte transformations in leucocyte cultures from malarious, malnourished and control Gambian children.


Deficit of spatial reversal in monkey with frontal lesions.


Some physiological and behavioural changes associated with oestrus and pregnancy in the squirrel monkey (Saimiri sciureus).


Glutaraldehyde fixation of boar spermatozoa for acrosome evaluation.


Radiosensitivity of synchronized Tetrahymena pyriformis W. cells.


A proline-dependent ATP-32Pi exchange in Bacillus sutilis B3 producing  mycobacillin.


Seasonal variation in personal health expenditure of rural populations.


Comparative sensitivity of suckling mice and Vero cells for primary isolation of chikungunya virus.


Studies on host-parasite relationships of Ilan strain of Schistosoma japonicum in animals. 2. Worm recovery rate.


The effects of intravenous protein--calorie supplementation on the tissue composition of postoperative body weight loss.


Successful resuscitation in diabetic ketoacidosis: a strong case for the use of bicarbonate.


Lactic acidosis and hyperamylasaemia associated with phenformin therapy.


Gonococcaemia with arthritis, dermatitis and myocarditis.


Relationships between intraocular pressure and the morphology of the outflow apparatus.


Neurologic effects of repeated exposures to high-pressure heliox atmospheres.


Biosynthesis of rat small seminal fructose as influenced by in vitro tissue excitation.


Clinical observation of the prevention of RH isoimmunization with immunoglobulin anti-D.


Protein malnutrition: correlation between anthropometric and biochemical assessment. Three hundred and eighty-six children aged 12-59 months, suffering from protein-calorie malnutrition were studied. Middle arm circumference and triceps skin-fold measurements were taken and from these values, middle arm muscular circumference was calclated. Simultaneously, the serum amino-acid balance was determined. The same measurements were carried out in 132 healthy control children of the same age group. These showed that middle arm muscular circumference does not change within the age groups studied, and with its value the amino-acid balance showed an inverse correlation. It is concluded that the severity of protein imbalance in malnutrition can be estimated on the basis of both middle arm muscular circumference and amino-acid balance. In view of its simple technique the former parameter is given preference.


Metabolic and hormonal effects of alternate infusion of hypertonic glucose and aminosol-glucose in premature infants. The metabolic and hormonal effects of a short term (24-36 hrs), alternating intravenous administration of hypertonic (16%) glucose and Aminosol-glucose were investigated in premature infants. The majority of the infants under 1 500 g showed pathological signs and did not tolerate oral feeding. The purpose of the study was to explore an intravenous alimentation program capable of ensuring an improved amino acid utilization in terms of nitrogen balance, and to prevent some of the adverse effects of the amino acid mixture. It was thought that the hormonal state created by 16% glucose might increase the capacity of the tissues to utilize the amino acids. The relevant findings were as follows. 1. Plasma free fatty acids and amino acids decreased, plasma insulin and growth hormone increased markedly in response to 16% glucose. The plasma aminogram indicated a profound redistribution of amino acids. 2. Aminosol-glucose infusion after the 16% glucose, increased the concentration of amino acids, but only to the starting level. Thus the previously decreased alpha-amino nitrogen content allowed to avoid a hyperaminoacidaemia and a marked distortion of the plasma amino acid pattern. Administration of the amino acid-glucose mixture usually failed to maintain the previously stimulated insulin and growth hormone secretion. 3. The hormonal and metabolis responses to the intermittent infusion of hypertonic glucose and Aminosol-glucose were not associated with an improved nitrogen utilization. More than 50% of the administered amino acid nitrogen was excreted. 4. The metabolic acidosis observed under these condition was found to be variable, and practically compensated.


The lethal interaction between the genes prune (pn) and killer of prune (Kpn) in Drosophila melanogaster.


Toxicity and mutagenicity of dimethyl sulfoxide in two strains of Drosophila melanogaster.


Newborn genetic screening as a concept in health care delivery: a critique.


Mass screening and genetic counseling in mendelian disorders.


Circulating tissue antigens. I. Tissue antigens in serum of patients with diseases involving injury of the liver and of other organs.


Plasma volume expansion and increased serum viscosity in myeloma and macroglobulinaemia.


The autosomal recessive mode of inheritance of C1r deficiency in a large Puerto Rican family.


Heterophilic antibodies in relation to malarial infection: population and experimental studies.


Comparative study on two labelled antimonial drugs, Bilharcid and tartar emetic using monkeys.


The innervation of the human vestibular sensory regions.


The fate and behaviour of antimonial drugs labelled with 124Sb in bilharzial subjets.


Xenografts.

Xenografts.


Porcine aortic valves for replacement of human heart valves.


Viral cardiomyopathy.

Viral cardiomyopathy.


Pathology of Chagas' cardiomyopathy.

Pathology of Chagas' cardiomyopathy.


Experimental cardiomyopathies in poikilotherms.


Natural history of diverse cardiomyopathies.


Lepers, madmen--who's next?

Lepers, madmen--who's next?


The diagnosis and psychopathology of schizophrenia in New York and London.


Study of protein reactivity in rat liver ribosomes. Effect of RNA addition to autodigested ribosomes.


Studies of the RNA and protein moieties of the larger subribosomal particle of rabbit reticulocytes.


Hydatid cyst of the brain as an epileptogenic factor.


Effect of epileptogenic stimulation on the acetylcholine content of rat hippocampus.


Some aspects of immunity in patients with cystic fibrosis.


Studies of serum complement in the hypocomplementaemic nephritides.


Diffuse normolipaemic plane xanthomatosis. An abnormal complement component profile.


The immunohistological detection of intracellular immunoglobulin in formalin-paraffin sections from multiple myeloma and related conditions using the immunoperoxidase technique.


Estrogen-binding in vitro by DMBA-induced rat mammary tumors: its relationship to hormone responsiveness.


About the normal values of the acid-base and oxygen indices in small laboratory animals--rats and rabbits.


Endogenic humoral effects on the formation of antitoxins.


A radiographic study of the ethmoidal latticed labyrinths from birth to the age of thirteen.


Histochemical studies of some dehydrogenases in dental tooth germ in vivo and in culture.


Relationship between the respiratory and lipopexic function of the lungs in some non-specific lung diseases depending on age.


Changes in glucose regulation following stomach resection according to Billroth II.


Changes in cholinesterase activity in white rats, after several injections of TMB-4 and atropine, following treatment with parathion.


Development of trichomonas vaginalis upon a medium "Trimed" containing serum of various animal origin.


Juniper bath treatment of the neurasthenic neurosis.


Schemes on the application of BCG to skin cancer.


Radio-active gold as an indicator in the study of the laxative effectiveness of some medicines.


Comparison between the results of the 131I--rose bengal functional examination and the scintigraphia in patients with liver diseases.


Congenital lobar emphysema--pathoanatomic studies of three cases.


Forensic medical and forensic chemical study of acute lethal poisinnins with thiodan.


Transepithelial potential difference in toad urinary bladder is not due to ionic diffusion.


Adjuvant activity in delayed hypersensitivity of the peptidic part of bacterial peptidoglycans.


Effects of dietary supplementation on the humoral immune response of malnourished mice.


Side effects of clonazepam therapy.

Side effects of clonazepam therapy.


Hypercalcaemia and epilepsy.

Hypercalcaemia and epilepsy.


Partial status epilepticus with speech arrest.


Neuro-ophthalmic deterioration after burns.


Damage to the inner ear in mastoid surgery.


Sex-dependent behavioral effects of cerebral cortical lesions in the developing rhesus monkey. Male rhesus monkeys with orbital prefrontal lesions were imtpaired on behavioral tests at 2(1/2) months of age whereas similar deficits were not detected in females with comparable lesions until 15 to 18 months of age. The results suggest that the maturation of a cortical region in the primate brain proceeds at different tempos in males and females.


Size and scaling in human evolution.

Size and scaling in human evolution. Our general conclusion is simply stated: many lineages display phyletic size increase; allometric changes almost always accompany increase in body size. We cannot judge adaptation until we separate such changes into those required by increasing size and those serving as special adaptations to changing environments. In our view, the three australopithecines are, in a number of features, scaled variants of the "same" animal. In these characters, A. africanus is no more "advanced" than the larger, more robust forms. The one early hominid to show a significant departure from this adaptive pattern toward later hominids-cranially, dentally, and postcranially-is H. habilis from East Africa. The australopithecines, one of which was probably a precursor of the Homolineage, were apparently a successful group of basically vegetarian hominids, more advanced behaviorally than apes (87), but not hunter-gatherers. The fossil hominids of Africa fall into two major groupings. One probable lineage, the australopithecines, apparently became extinct without issue; the other evolved to modern man. Both groups displayed steady increase in body size. We consider quantitatively two key characters of the hominid skull: cranial capacity and cheek tooth size. The variables are allometrically related to body size in both lineages. In australopithecines, the manner of relative growth neatly meets the predictions for functional equivalence over a wide range of sizes (negative allometry of cranial capacity with a slope against body weight of 0.2 to 0.4 and positive allometry of postcanine area with a slope near 0.75). In the A. africanus to H. sapiens lineage, cranial capacity increases with positive allometry (slope 1.73) while cheek teeth decrease absolutely (slope - 0.725). Clearly, these are special adaptations unrelated to the physical requirements of increasing body size. We examined qualitatively other features, which also seem to vary allometrically. Of course, many characters should be studied quantitatively, but we think that the scheme outlined here should be treated as the null hypothesis to be disproved.


Neural basis of orientation perception in primate vision. Orientational differences in human visual acuity can be related parametrically to the distribution of optimal orientations for the receptive fields of neurons in the striate cortex of the rhesus monkey. Both behavioral measures of acuity and the distribution of receptive fields exhibit maximums for stimuli horizontal or vertical relative to the retina; the effect diminishes with distance from the fovea. The anisotropy in the neuronal population and in visual acuity appear to be determined by postnatal visual experience.


The use of a diphosphonate in para-articular ossifications after total hip replacement. A clinical study.


Autoperfusing heart-lung preparation: prolonged survival by extracorporeal symbiosis.


The relationship between precursor and mature forms of the 23S ribosomal RNA.


Comparison of RNA sequences from widely divergent species.


A new isozyme of triose phosphate isomerase specific to hominoids.


Doublet frequency analysis of bacterial DNAs.


The isolation and identification of an antigen for the diagnosis of bovine mastitis by radial immunodiffusion.


Vibrations of the temporal bone due to head injury.


A long-term study of children born to diabetic mothers. (With special reference to occurrence of late neurological abnormalities).


Parenteral hyperalimentation: a beginning.


Intravenous Additive program: progress at Naval Hospital Portsmouth.


Prostaglandins in nephrology.

Prostaglandins in nephrology.


Morphology and serum complement levels in membranoproliferative glomerulonephritis.


Parathyroid function in renal insufficiency.


Influence of the preservation technique on the early function of the kidney after reimplantation.


The functional significance of complement.


Are there contraindications to renal transplantation?


Recent drug management advances in renal insufficiency.


The nature and pathogenesis of systemic amyloidosis.


Renal insufficiency in lupus nephritis.

Renal insufficiency in lupus nephritis.


Complement deposition in the kidney.

Complement deposition in the kidney.


Evidence for activation of the alternate pathway in glomerulonephritis.


Complement and mesangiocapillary glomerulonephritis: the role of complement deficiency in glomerulonephritis.


Activation of complement in human nephritis.


The C3 nephritic factor (C3NeF) and the heat labile complement inactivator (HLCI) in chronic hypocomplementemic mesangioproliferative glomerulonephritis.


M--craniocarpotarsal dystrophy (whistling face syndrome) in two families.


N--variable expressivity in craniocarpotarsal dysplasia.


S--the whistling-face syndrome: report of a case with a renal anomaly.


The REEDS syndrome.

The REEDS syndrome.


The Roberts syndrome.

The Roberts syndrome. The Roberts syndrome appears to follow an autosomal recessive mode of inheritance. The cause of the abnormalities probably exerts its effect prior to the seventh week of gestation and only on a few organ systems undergoing development during this time. The peculiar morphologic features of metaphase chromosomes of the one individual who has been studied extensively are unexplained.


Some bacteriological considerations of soft lens wear.


Gonadotropin and prolactin secretion in human pseudocyesis: effect of synthetic luteinizing hormone-releasing hormone (LH-RH) and thyrotropin releasing hormone (TRH)


Biophysical intrapartal fetal monitoring.


Neurologic problems in perinatology.

Neurologic problems in perinatology.


Radioimmunoassay of plasma LH in rat: basal levels and effects of synthetic LH-RH.


Gel filtration of thyroxine--binding proteins. Identification of human serum protein fractions separated on Sephadex G 100.


A correlative study of thyroid mast cells and thyroid function in the rat.


Gel filtration of thyroxine--binding proteins. Identification of rat plasma protein fractions separated on Sephadex gels.


Observations on plaque morphology of Pseudomonas pyocynea bacteriophages.


Pathophysiological role of plasma kinins in malaria.


[D-cycloserine and germination of Bacillus cereus spores].


Presubiculum afferents to the entorhinal area and the Papez circuit.


Projections of auditory responsive cortex in the squirrel monkey.


Interhemispheric communication between extraoccipital visual areas in the monkey.


Certain biochemical correlates of intense serotonin histofluorescence in the brain stem of the neonatal monkey.


Impaired orientation after primate tectal lesions.


The role of the institution and the concept of parallel services.


Some issues in manpower for parallel services.


Immunochemical studies of brain glutamate decarboxylase and GABA-transaminase of six inbred strains of mice. Six different inbred strains of mice (C57BL/6J, CBA/CaJ, CE/J, DBA/2J, LP/J and RF/J) were compared in terms of specific activities and immunochemical properties of brain L-glutamate decarboxylase (GAD) and gamma-aminobutyrate transaminase (GABA-T), the enzymes responsible for the synthesis and degradation of GABA, respectively. GAD from the brains of the different strains was indistinguishable on the basis of specific activities, double diffusion tests, immunoelectrophoresis and inhibition by antibody. However, microcomplement fixation tests showed GAD from DBA and C57BL mice to be most distinctly different from GAD extracted from the Swiss mouse, from which the original antigen was prepared and that the enzyme from the CE, LP and RF also differed. Similar fixation curves were obtained for the GAD from CBA and Swiss mice. GABA-T from the different strains was indistinguishable on the basis of all the tests employed.


Cost-effective analysis. A quandary for geriatric health care systems.


Symposium--long-time care: research, policy, and practice. Relocation research and social policy.


Approaches to improving the quality of long-term care for older persons.


Specific granules in human and nonhuman vertebrate cardiocytes.


Sodium cromoglycate 2 per cent solution in perennial rhinitis: a clinical and histological study.


Proceedings: Macroscopic organisation of Ligamenta flava in cercopithercinae.


Proceedings: Vitamin deficiencies and brain metabolism.


Serum diamine oxidase in pregnancy.

Serum diamine oxidase in pregnancy.


The significance of dental epithelium in the pathogenesis of periodontal disease.


Cutis marmorata telangiectatica congenita.


Acanthosis nigricans and hypovitaminosis A. response to topical vitamin A acid.


A special tupaia cage for the continuous detection of locomotor activity under the auto- or the self-control of light-darkness rhythms.


Transduction of a streptomycin R-factor from Mycobacterium smegmatis to Mycobacterium tuberculosis H37Rv.


A new case-finding programme in a region of Taiwan.


The meaning and structure of species as applied to mycobacteria.


A family outbreak of tuberculosis in Bristol in 1973 with two cases of tuberculous meningitis.


A study in Hong Kong to evaluate the role of pretreatment susceptibility tests in the selection of regimens of chemotherapy for pulmonary tuberculosis--second report. A Hong Kong Tuberculosis Treatment Services/British Medical Research Council Investigation.


A study of delayed hypersensitivity, lymphocyte transformation and leucocyte migration inhibition by M. xenopi and M. tuberculosis in patients harbouring these organisms.


Comparative trials of British  BCG vaccine double strength, and Danish BCG vaccine, with standard strength British BCG vaccine.


Mycobacterium gadium sp. nov. a new species of rapid-growing scotochromogenic mycobacteria.


Congenital anomalies of the lower urinary tract from a radiologic point of view. Congenital anomalies of the lower urinary tract are being recognized with greater frequency due to the use of micturating cystourethrography. Many of these malformations present with urinary tract infection. Early diagnosis is important as appropriate treatment may prevent deterioration of renal function. Several illustrative cases are presented, such as posterior urethral valves, diverticula of the urethra, rectourethral fistula, and duplication of the urethra.


Aspartylglycosaminuria: a gargoyle-like syndrome with autosomal recessive inheritance. Aspartylglycosaminuria is an autosomal recessive disorder of glycoprotein catabolism, characterized by presence of aspartyglycosamine in the urine, progressive mental retardation, coarse face, impaired speech and motor functions, and signs of involvement of connective tissue and skeleton. In infancy, clinical symptoms are mild or absent. Vacuolized lymphocytes are often found in the blood and bone marrow. The disease appears unusually common in Finland.


Ingestion of asbestos.

Ingestion of asbestos.


The toxicity of sodium nitroprusside.

The toxicity of sodium nitroprusside.


Local and systemic subarachnoid toxicity of metrizamide in mice and monkeys.


Hycanthone: a review of its experimental chemotherapy, pharmacology and toxicology.


Chloroplast- and cytoplasm-specific aminoacyl-transfer ribonucleic acid synthetases of Euglena gracilis: separation, characterization and site of synthesis.


Retinol-binding proteins.

Retinol-binding proteins.


A study of the feeding and social behaviour of the "Macacus rhesus" monkey after direct electrical stimulation and telestimulation of different points of the amygdala and the hippocampus. We examined first the behavior of a single animal (Macacus Rhesus), partially free to move on a Brady chair, before and after direct electrical stimulation of the amygdala and the hippocampus; then the social behavior of two animals, completely free to move in a large behavioral cage, before and after stimulation, by radio, of the amygdala. Certain ventromedial areas of the amygdala have a short and long term inhibitory effect on feeding and social behavior and the hippocampus has a short and long term facilitating effect on feeding behavior, especially during the after discharge.


Calcium ions and the function of the contractile proteins of muscle.


Calcium movements in relation to contraction.


Effects of administering cold and warmed tube feedings.


A physiological and behavioral approach to understanding the mechanisms of obesity and anorexia.


Critique of "A physiological and behavioral approach to understanding the mechanisms of obesity and anorexia".


The effects of oestrogens on the sexual behaviour of the femal rhesus monkey.


Photosensitive epilepsy in Papio papio. Possible role of cerebellar lesions on facilitation of seizure discharges.


The effects of oestrogen-treatment of the female on the behaviour of the male rhesus monkey.


Progressive seizure recruitment in subhuman primates and effect of cerebellar stimulation upon developed vs. developing amygdaloid seizures.


Oral manifestations of von Reclinghausen's disease: report of a case.


Pathological problems around the placental separation.


Hodgkin's disease and the non-Hodgkin's lymphomas in childhood.


Transfer-factor therapy of malignancy.

Transfer-factor therapy of malignancy.


The chemical therapy of breast cancer.

The chemical therapy of breast cancer.


Comparative histology of the sustentacular organs in the tongues of the mammalia.


The expression of inherited metabolic disease in cultured cells.


Use of microtiter and agar-gel diffusion techniques in screening avian serums for influenza A and Myxovirus yucaipa antibodies.


Prosthodontics in Japan 1973-1974.

Prosthodontics in Japan 1973-1974.


Characteristics of rat normal mammary epithelial cells and dimethylbenzanthracene-induced mammary adenocarcinoma cells grown in monolayer culture.


Oral surgery in Japan 1973-1974.

Oral surgery in Japan 1973-1974.


Pressure-volume curves and closing volume.


The rotation of the maxilla.

The rotation of the maxilla.


Long term observation on the extraction of second permanent molars.


Milk hygiene and bacteriology in the Sudan: isolation of brucella abortus from cow's milk.


Testing acaricide susceptibility of the brown dog tick Rhipicephalus sanguineus (Latreille, 1806). II Teabag method.


A comparative study of vestibulocortical projection.


The vestibular sensory cells and their innervation.


Response patterns of vestibular thalamic neurons in the rhesus monkey.


Effects of labyrinthectomy on optokinetic nystagmus (OKN) and optokinetic after-nystagmus (OKAN). OKN and OKAN were affected by unilateral and bilateral labyrinthectomy. The maximum velocity of slow phases of OKN induced by drum rotations above 60-75 degrees/sec in either direction was lower after unilateral and bilateral labyrinthectomy. The frequency of OKN was also decreased, and the total deviation of the eyes was reduced for OKN induced by these drum speeds. Changes in OKN eventually recovered. OKAN was reduced in duration after unilateral labyrinthectomy, being initially shorter to the ipsilateral than to the contralateral side. OKAN could no longer be evoked after bilateral labyrinthectomy. This loss was permanent. These data demonstrate the importance of the vestibular system in maintenance of OKN and OKAN.


Experimental study on abnormal habits and malocclusion (part III).


Non-hormonal methods of antenatal monitoring.


Fetal acid-base balance.

Fetal acid-base balance.


The functional occlusion of the teeth of the Vervet monkey.


Combination of triamcinolone acetonide and demethylchlortetracycline hydrochloride in the management of pulp hyperemia and acute serous pulpitis.


Investment bonds for profit.

Investment bonds for profit.


The healing of extraction wound in the presence of retained root remnants. Clinical, radiographical and histological study.


Epidemiology of Chagas' disease in the state of Ceará, Brazil. IV. The role of dogs and cats as domestic reservoir.