Extrachromosomal elements in group N streptococci. The deoxyribonucleic acid (DNA) of Streptococcus lactis C2, S. cremoris B(1), and S. diacetilactis 18-16 was labeled by growing cells in Trypticase soy broth containing (3)H-labeled thymine. The cells were gently lysed with lysozyme, ethylenediaminetetraacetic acid, and sodium lauryl sulfate. The chromosomal DNA was separated from plasmid DNA by precipitation with 1.0 M sodium chloride. The existence of covalently closed circular DNA in the three organisms was shown by cesium chloride-ethidium bromide equilibrium density gradient centrifugation of the cleared lysate material. In an attempt to correlate the loss of lactose metabolism with the loss of plasmid DNA, lactose-negative mutants of these organisms were examined for the presence of extrachromosomal particles. Covalently closed circular DNA was detected in the lactose-negative mutants of S. lactis C2 and S. diacetilactis 18-16. In S. cremoris B(1), however, no covalently closed circular DNA was observed by using cesium chloride-ethidium bromide gradients. Electron micrographs of the satellite band material from S. lactis C2 and its lactose-negative mutant confirmed the presence of plasmid DNA. Three distinct plasmids having approximate molecular weights of 1.3 x 10(6), 2.1 x 10(6), and 5.1 x 10(6) were observed in both organisms.


Size distribution of the closed circular deoxyribonucleic acid molecules of Bacillus megaterium: sedimentation velocity and electron microscope measurements. The circular deoxyribonucleic acid (DNA) of Bacillus megaterium was fractionated by sedimentation velocity on preparative zonal gradients. The fractions obtained were characterized by analytical sedimentation velocity analysis on neutral and alkaline sucrose gradients, and by contour length measurement by electron microscopy. Each fraction was found to contain circular molecules of one to three discrete sizes, in various combinations of covalently closed and open circular forms. Estimations of the molecular masses of these sizes gave values of 3.9, 6.2, 16.0, 31, and 60 million daltons for the major molecular species. Minor amounts of molecules of 7.6, 11.7, 47, 89, and 112 million daltons were observed in the electron microscope analyses. Length measurements of almost 600 molecules from the various fractions showed that all except six could be placed in distinct size classes. The distribution of molecular sizes in unfractionated circular DNA was shown to consist primarily of the two smallest size classes, although the relative proportions by weight of five of the classes were roughly equivalent.


Magnesium transport in Bacillus subtilis W23 during growth and sporulation. The active transport of magnesium by cells of Bacillus subtilis strain W23 occurs by a highly specific transport system (Mg(2+) is favored over Mn(2+), Co(2+), or Ca(2+)) that is energy dependent (i.e., glucose is required in minimal medium and the system is inhibited by cyanide and m-chlorophenyl carbonylcyanidehydrazone). The rate of magnesium uptake by log-phase B. subtilis cells follows saturation kinetics with a K(m) of 2.5 x 10(-4) M and a V(max) of 4.4 mumol per min per g (dry weight) at 30 C. Manganese is a competitive inhibitor showing a K(i) of 5 x 10(-4) M. During sporulation the rate of magnesium transport declines. This decline in rate is specific for the magnesium system as the manganese and calcium transport rates increase. The residual magnesium transport function in sporulating cells shows both an altered K(m) and an altered V(max). The magnesium content of late sporulating cells is also lower than that for log-phase cells.


Transport of glucose, gluconate, and methyl alpha-D-glucoside by Pseudomonas aeruginosa. Glucose transport by Pseudomonas aeruginosa was studied. These studies were enhanced by the use of a mutant, strain PAO 57, which was unable to grow on glucose but which formed the inducible glucose transport system when grown in media containing glucose or other inducers such as 2-deoxy-d-glucose. Both PAO 57 and parental strain PAO transported glucose with an apparent K(m) of 7 muM. Free glucose was concentrated intracellularly by P. aeruginosa PAO 57 over 200-fold above the external level. These data constitute direct evidence that glucose is transported via active transport by P. aeruginosa. Various experimental data clearly indicated that P. aeruginosa PAO transported methyl alpha-d-glucose (alpha-MeGlc) via the glucose transport system. The apparent K(m) of alpha-MeGlc transport was 7 mM which indicated a 1,000-fold lower affinity of the glucose transport system for alpha-MeGlc than for glucose. While only unchanged alpha-MeGlc was detected intracellularly in P. aeruginosa, alpha-MeGlc was actually concentrated intracellularly less than 2-fold over the external level. Membrane vesicles of P. aeruginosa PAO retained transport activity for gluconate. This solute was concentrated intravesicularly several-fold over the external level. A component of the glucose transport system is believed to have been lost during vesicle preparation since glucose per se was not transported. Instead; glucose was converted to gluconate by membrane-associated glucose dehydrogenase and gluconate was then transported into the vesicles. Although this may constitute an alternate system for glucose transport, it is not a necessary prerequisite for glucose transport by intact cells since P. aeruginosa PAO 57, which lacks glucose dehydrogenase, was able to transport glucose at a rate equal to the parental strain.


Regulation of aconitase synthesis in Bacillus subtilis: induction, feedback repression, and catabolite repression. The synthesis of aconitase in Bacillus subtilis wild-type and different citric acid cycle mutants has been studied and the influence of various growth conditions examined. Aconitase is induced by citrate and precursors of citrate and repressed by glutamate. Induction and repression counteract each other, and at equimolar concentrations of citrate and glutamate, aconitase synthesis is unaffected. Induction by citrate can partly overcome catabolite repression of aconitase. Isocitrate dehydrogenase show endogenous induction of aconitase due to citrate accumulation. Leaky mutants defective in citrate synthase and aconitase cannot be induced by citrate, which indicates that they carry a regulatory mutation. The complex regulation of aconitase is discussed with reference to the participation of this enzyme in glutamate biosynthesis and energy metabolism.


Cell wall turnover at the hemispherical caps of Bacillus subtilis. Cell walls made by Bacillus subtilis bacteria grown in D(2)O medium have buoyant densities in CsCl which are different from walls made by cells grown in H(2)O medium. Cell wall turnover was studied by measuring the change in wall buoyant density after a B. subtilis culture was shifted from growth in D(2)O medium to aeration in H(2)O medium. Walls from the hemispherical caps were isolated after preferential digestion of wall from the cylindrical regions using the B. subtilis autolytic amidase. The walls from the polar regions were found to turn over extensively.


Physiological studies of Bacillus subtilis minicells. Minicells produced by Bacillus subtilis strains carrying the div IV-B1 mutation, (CU 403 div IV-B1 and CU 403 div IV-B1, tag-1), were purified by a procedure which destroys parental cells with ultrasound, but spares minicells. Such preparations generally contain 10(9) or more minicells/ml and less than 10(4) colony-forming units/ml. Purified minicells are resistant to autolysis in tris(hydroxymethyl)aminomethane buffer, pH 7.5, at 30 C, conditions which result in total lysis of parental cells. Minicells are not completely devoid of autolytic activity, however. The medium in which minicells are produced, the temperature at which purified minicells are incubated, and the genotype of cells from which the minicells are derived all influence the rate of autolysis of purified minicells. These parameters are demonstrated by using minicells obtained from div IV-B1 and div IV-B1, tag-1 strains. Ultrastructural differences have been observed in the products of autolysis of these two minicell strains. Minicells are sensitive to low levels of lysozyme and yield miniprotoplasts when the wall is removed in an osmotically protective environment. Although minicells are unable to grow, they can maintain their integrity over long periods of time, which suggests functional energy metabolism in minicells. Direct measurements of adenosine 5'-triphosphate (ATP) levels by the luciferase assay indicated that minicells can produce ATP. Oxygen consumption, measured by standard respirometry techniques, also indicates functional metabolism in minicells. These findings demonstrate that minicells purified by ultrasound are suitable material for study of physiological processes in anucleate cells.


Chemical composition and ultrastructure of native and reaggregated membranes from protoplasts of Bacillus cereus. Conditions were defined for producing protoplasts with lysozyme and isolating the protoplast membranes from cells of Bacillus cereus T harvested late in the exponential growth phase just before sporogenesis. The membranes contained approximately 60% protein, 30% lipid, 6% carbohydrate, and 1% ribonucleic acid. Seventeen proteins were distinguished by molecular size in the membrane solubilized with sodium dodecyl sulfate, and 12 in that with phenol and acetic acid. The lipid fraction consisted of neutral lipids (28%) and phospholipids (72%). Four phospholipids were identified: diphosphatidyl glycerol, phosphatidyl ethanolamine, phosphatidyl glycerol, and lysophosphatidyl ethanolamine. Eighteen fatty acids were identified, with a predominance of branched C(15) and C(17) and of normal C(16) acids. The carbohydrate fraction consisted of neutral hexoses. A clear supernatant solution from the solubilized preparation became reaggregated into membrane by dialysis in the presence of MgCl(2). The reaggregated membrane had the same main components as the native membrane, but the amount and ratio of protein and lipid depended on the buffer and the MgCl(2) concentration. By electron microscopy, the reaggregated membranes appeared as vesicles or sheets, depending on the MgCl(2) concentration. Hexagonal lattices were occasionally detected in the negatively stained ultrastructure of both native and reaggregated membrane fragments.


Degradation of thymidine by Lactobacillus acidophilus. Whole cells of Lactobacillus acidophilus are capable of degrading thymidine to thymine, suggesting the presence of thymidine phosphorylase (or thymidine hydrolase). This activity was also demonstrated in cell-free extracts.


Degree of completion of 3'-terminus of transfer ribonucleic acids of Bacillus subtilis 168 at various developmental stages and asporogenous mutants. Transfer ribonucleic acids from sporulating cells, spores, sporangia, or stationary-phase asporogenous mutants of Bacillus subtilis all showed a deficiency in the 3'-terminal adenosine moiety.


The effect of anti-plasma cell serum on B cells.


Characteristics of secretory immune system in human middle ear: implications in otitis media.


Early appearance of sensitized lymphocytes in mice infected with Listeria monocytogenes.


Polyribitol-phosphate: an antigen of four gram-positive bacteria cross-reactive with the capsular polysaccharide of haemophilus influenzae type B.


The role of T lymphocytes in the primary humoral antibody response to brucellin.


Activation of the classic and alternate complement pathways by endotoxin.


Cytotoxic antibodies and lymphocytes induced by orthotopic tooth bud allografts in mice.


Interaction of complement with antigen-antibody complexes of rabbit allotypic antigens and their corresponding antibodies.


Immunosuppressive effects of anti-lymphocyte serum in complement deficient mice. II. Evidence that activity is mediated by C3 independent antibodies.


Hypothalamic input to supraoptic neurons.


Grey's Hospital: the oldest hospital on its original site in the Republic.


Immunofluorescent demonstration of bacterial antigen in experimental pyelonephritis with antiserum against common enterobacterial antigen.


A new federal financial formula: an interview with the Minister of National Health and Welfare.


Psychiatric care of the disadvantaged child in a private practice setting.


Suppressive effect of concurrent administration of metal salts on carcinogenesis by 3'-methyl-4-(dimethylamino)azobenzene, and the effect of these metals on aminoazo dye metabolism during carcinogenesis.


Disturbance of immune system in mice by administration of 7,12-dimethylbenz(a)anthracene and normal thymocytes.


Growth of the blue-green alga Tolypothrix tenuis as affected by cobalt and molybdenum.


Effect of copper on the composition of bacterial cell wall peptides.


Production of bacterial amylase on some commercial materials.


Optimal conditions for production of bacterial amylase.


Relationship between soybean cultivars and Rhizobium japonicum serotypes with single- and multi-strain inoculants. I. Greenhouse pot experiments.


Further investigations on hydroxyproline excretion in bovine puerperal paresis.


The influence of selenium administration on copper levels and growth of lambs.


On the biometry of spermatozoa from different levels of the reproductive tract of buffalo bulls.


A kininogen-kinin like reaction revealed by the marginal distance of the equine lung.


Histological and histoenzymochemical aspects of ocular tumours in cattle.


Cysts of the spinal ganglia.

Cysts of the spinal ganglia.


Laboratory animal anaesthesia.

Laboratory animal anaesthesia.


New appraoch to save health care costs.

New appraoch to save health care costs.


Toxoplasmosis: an overview.

Toxoplasmosis: an overview.


Nonrheumatic valvular cardiac disease: a clinicopathologic survey of 27 different conditions causing valvular dysfunction.


Differential effects of chromonar and nitroglycerin on intramyocardial oxygen tension.


Vaccinia virus morphogenesis: a comparison of virus-induced antigens and polypeptides.


A method of freeze-drying and fixing spermatozoa for electron microscopy.


The effect of diphosphonate on calcium phosphate crystallization in urine in vitro.


The kinetics of synthesis and degradation of aspartate aminotransferase isozymes in rat peripheral red blood cells during cytodifferentiation.


Blood group antigens I, i, H and HI on monkey, rabbit and guinea pig erythrocytes.


The effect of intravenous hyperalimentation on erythrocyte lipids.


Tyrosinase activity in a highly pigmented human melanoma and in negro skin.


Prevention of microbial catheter contamination in patients receiving parenteral hyperalimentation.


Primary reticulum cell sarcoma of the femur: 12-year follow-up after radiation therapy and pathologic fracture.


In vitro specificity and in vivo activity of antimacrophage serum.


On the levels of hemoglobins F and A2 in sickle-cell anemia and some related disorders.


Total balanced intravenous feeding by peripheral veins in paediatric surgery. A summary of 7 years' research and clinical experience.


Insecticides, polychlorinated biphenyls and mercury in wild cormorants, pelicans, their eggs, food and environment.


Inhibition of microsomal drug metabolism by anticholinesterase insecticides.


Uptake and accumulation of DDT and PCB by Ephemera danica (Ephemeroptera) in continuous-flow systems.


Molybdenum toxicity: abnormal cellular division of teratogenic appearance in Euglena gracilis.


Chlorinated hydrocarbons in plankton from the Gulf of Mexico and Northern Carribbean.


Discharge planning; an asset in the continuum of patient care services.


The HMO in perspective.

The HMO in perspective.


IgE and IgG antibodies against Aspergillus fumigatus in sera of patients with bronchopulmonary allergic aspergillosis.


Immuno- and physicochemical studies of an IgA-IgG mixed cryoglobulin.


Limb immobilization devices for chaired waking monkeys.


Industrial chemicals. Extraction of polychlorinated biphenyls: evaluation of a column technique applied to polar bear and seal tissue.


Folate coenzymes of Lactobacillus casei and Streptococcus faecalis.


Kinetic mechanism of glycogen synthase D from human polymorphonuclear leukocytes.


Homocysteine biosynthesis in green plants. O-Phosphorylhomoserine as the physiological substrate for cystathionine gamma-synthase.


The carboxypeptidase-dependent inactivation of thymidylate synthetase.


Initiation of protein synthesis by folate-sufficient and folate-deficient Streptococcus faecalis R. Biochemical and biophysical properties of methionine transfer ribonucleic acid.


Separation of dihydroergotoxine (hydergine) into dihydroergocornine, dihydroergocryptine and dihydroergocristine by counter-current distribution.


Studies of membrane formation in Tetrahymena pyriformis. The biosynthesis of proteins and their assembly into membranes of growing cells.


Functional localization in the trigeminal root.


Whirlpool therapy for the treatment of soft tissue wounds complicating extremity fractures.


Letter: Urethritis at a regional VD clinic.


Cryosurgery of respiratory structures. II. Cryonecrosis of the lung.


Another generation gap: physicians vs. the elderly.


Low dose prophylactic antibiotics in vaginal hysterectomy.


The effect of anticoagulants on the dialysis method of measurement of vitamin B 12 binding capacity of plasma proteins.


Diagnosis and incidence of Neisseria gonorrhoeae in Cape coloured females in the Western Cape. Laboratory aspects.


Metabolism of 14C-histamine in amphibians (Bufo bufo) and reptiles (Pseudemys scripta and Testudo hermanni).


Application of Cu(II) ions for an indicator reaction in volumetric methods of drug assay. VI. Quantitative determination of the active substance in spasmophen and spasmophen-duplex.


A comparison of retinal argon laser lesions in man and in cynomolgus monkey.


Double-mind theory.

Double-mind theory.


Primary dietary prevention of atherosclerosis.


Hospital food and possibilities of its improvement. Clinical aspects.


Biosynthesis of immunoglobulin A (IgA). Secretion and addition of carbohydrate to monomer and polymer forms of a mouse myeloma protein. Cell suspensions of mouse plasma-cell tumour MOPC 315 secreting predominantly IgA (immunoglobulin A) monomer and dimer were incubated with radioactive leucine, mannose, galactose and fucose for various periods of time. The amounts of secreted and intracellular immunoglobulins were measured by co-precipitation with specific antibody, and the molecular species present were assessed by electrophoresis in polyacrylamide gels. Analysis of the secreted myeloma protein demonstrated that monomer and dimer IgA molecules are identical with respect to carbohydrate composition and rate of secretion. Within the cell, the myeloma protein is almost entirely accounted for by monomer units which either leave the cell as such or are polymerized with the addition of J chain close to the time of secretion. The results support the concept of a stepwise addition of carbohydrate residues to IgA immunoglobulin during the process of secretion. Similar patterns of carbohydrate assembly were found for the monomer or dimer molecules. Mannose residues are added at an early stage, whereas fucose is added close to the time of secretion. Galactose is also added early, but some may also be incorporated at a later stage. Control of IgA polymerization is considered unlikely to reflect regulation at the level of carbohydrate addition, and it is suggested that the critical controlling factor is the J chain.


Type-A influenza viruses isolated from wild free-flying ducks in California.


Biosynthesis of immunoglobulin A (IgA) and immunoglobulin M (IgM). Control of polymerization by J chain. Cell suspensions of mouse plasma-cell tumours secreting IgA (immunoglobulin A) and IgM (immunoglobulin M) were incubated with radioactive leucine for various periods of time. The secreted immunoglobulins were precipitated from the culture medium with specific rabbit antisera to determine the relative distribution of radioactivity among the different molecular species, and to estimate the fraction of total radioactivity in the J chain. For IgM-secreting cells there is a balanced synthesis of 7S subunits and J chains, and the secreted product is uniformly assembled to the pentamer. In cells secreting IgA, however, the results demonstrate that the pool of intracellular J chain is less than the intracellular IgA pool. The concentration of J chain is therefore limiting and is less than the requirement for complete polymerization. The major factor that determines whether an intracellular monomer is secreted as such or is polymerized with the addition of J chain is therefore the amount of intracellular J chain. When this is limiting, as it is in cells secreting IgA, then monomer will be secreted.


Biosynthesis of immunoglobulin A (IgA) and immunoglobulin M (IgM). Requirement for J chain and a disulphide-exchanging enzyme for polymerization. Mouse myeloma cells secreting 19S IgM (immunoglobulin M) (MOPC 104E and TEPC 183) or monomer and polymer IgA (immunoglobulin A) (MOPC 315) were incubated with radioactive leucine and the intracellular and secreted immunoglobulins and immunoglobulin subunits were prepared by preparative sucrose-density-gradient centrifugation. Samples were reduced in the presence or absence of isolated J chain, passed over Sephadex G-25 and then incubated at 37 degrees C for 30min with or without a source of disulphide-interchange enzyme. The extent of reassembly of reduced subunits was then evaluated by electrophoresis in polyacrylamide gels. Provided that J chain and the disulphide-interchange enzyme were supplied, both IgM and IgA could be assembled from their respective subunits, obtained by reductive cleavage of polymeric forms. Under similar conditions, assembly of polymeric forms from intracellular or secreted 7S monomer subunits also occurred. Under these conditions polymerization was total, there being no residue of the monomeric form. Reassembly did not occur in the absence of either J chain or the enzyme. All of the J chain released from IgM by reductive cleavage was incorporated back into the reassembled polymer. The J chain is therefore likely to be an essential structural requirement for polymeric immunoglobulins. A variety of controls ruled out non-specific interactions, and further suggested that the amino acid sequence of polypeptide chains determines the specificity of polymerization. The fact that intracellular IgA and IgM monomer subunits known to be deficient in galactose and fucose can be completely polymerized suggests that the addition of carbohydrate does not control polymerization.


Effect of methylglyoxal bis(guanylhydrazone) on polyamine metabolism in normal and regenerating rat liver and rat thymus. 1. Injections of sublethal doses of methylglyoxal bis(guanylhydrazone), a potent inhibitor of putrescine-activated S-adenosylmethionine decarboxylase in vitro, resulted after a few days in an immense increase in the activity of S-adenosylmethionine decarboxylase in normal and regenerating rat liver and in rat thymus. The increase in the activity of S-adenosylmethionine decarboxylase was at least partly due to a marked lengthening of the half-life of the enzyme. 2. In regenerating liver and thymus there was also a moderate stimulation of the activity of ornithine decarboxylase (EC 4.1.1.17) and a marked accumulation of tissue putrescine. 3. Injection of methylglyoxal bis(guanylhydrazone) into the rat also markedly decreased the activity of diamine oxidase (EC 1.4.3.6) in thymus. 4. In no cases where doses of methylglyoxal bis(guanylhydrazone) close to the LD(50) dose for the rat were used was it possible to lower tissue spermidine content to any significant extent. 5. Methylglyoxal bis(guanylhydrazone) seemed to act as a competitive inhibitor for the substrate S-adenosylmethionine and as an uncompetitive inhibitor for the activator putrescine in the decarboxylation of S-adenosylmethionine in vitro. 6. In the diamine oxidase reaction, with putrescine as the substrate, methylglyoxal bis(guanylhydrazone) was a non-competitive inhibitor for putrescine.


A comparison of the prophylactic efficacy of lyophilized and cell-associated turkey herpesvirus against Marek's disease.


Effects of Eimeria acervulina on intestinal pH in conventional and gnotobiotic chickens.


Pathology and prevention of Leucocytozoon smithi infection of Turkeys.


Varying persistence of polychlorinated biphenyls in six California soils under laboratory conditions.


A search for polychlorinated biphenyls in human milk in rural Colorado.


Effects of pH, light and temperature on carbaryl in aqueous media.


Immunization against Marek's disease: influence of strain of chickens, maternal antibody, and type of vaccine.


Embryotoxic effects of three PCB'S in the chicken.


Tests of pesticidal synergism with young pheasants and Japanese quail.


PCB and hydrocarbon contamination of plankton by nets.


A qualitative method for detecting hydroxylated metabolites of polychlorinated biphenyls.


Outer (cell wall) membrane proteins of Pseudomonas aeruginosa.


"Whither medicine in Rhodesia".

"Whither medicine in Rhodesia".


Demonstration of delayed hypersensitivity to Neisseria meningitidis.


New thoughts for old. II.

New thoughts for old. II.


Comparison of preparations of erythrocyte membranes and membrane proteins by SDS-gel electrophoresis.


Precipitating antibody against core glycolipid of Enterobacteriaceae.


Orosomucoid, seromucoid and haptoglobin in serum during adjuvant arthritis of the rat.


Intracavitary electron beam therapy for cancer of the uterine cervix: dose-distribution study and clinical result.


Letter: Susceptibility to damage from impulse noise: chinchilla versus man or monkey.


Parenteral hyperalimentation in gynecologic oncology patients.


Antimetabolites produced by microorganisms. VII. L-(N5-phosphono)methionine-S-sulfoximinyl-L-alanyl-L-alanine.


Studies on the antiviral substance. Mechanism of action of bihoromycin, a new antibiotic.


Fluorescence studies on the interaction between heavy meromyosin and magnesium ions. Effect of EDTA.


Ionic changes during smooth muscle fixation for electron microscopy.


Identification and localization of intraarticular colloidal gold: ultrastructural and electron microprobe examinations of human biopsies.


The reticulum cell and the reticular cell in the mouse popliteal lymph node. An electron microscopic autoradiographic study.


Nephritogenic properties of nephrotoxic guinea pig antibodies. II. Glomerular lesions induced by F(ab')2 fragments of nephrotoxic IgG1 antibody in rats.


Effect of heparin, histamine and serotonin on the density-dependent inhibition of replication in two fibroblastic cell lines.


Hunger and satiety in humans during parenteral hyperalimentation.


Letter: Rhesus prophylaxis after twin delivery.


Kotonkan, a new rhabdovirus related to Mokola virus of the rabies serogroup.


Infectivity of sputum-positive tuberculous patients on chemotherapy.


Routine diagnosis of tuberculosis with phase-contrast microscopy.


Letter: Selective inhibition of phagocytic activity of rabbit alveolar macrophages by cystic fibrosis serum.


An electron microscopic observation of the nucleolar material attached to the chromosomes of HeLa cells in metaphase.


Osmium impregnation of methyl methacrylate vascular casts for scanning electron microscopy.


Microbial degradation of DDT metabolites to carbon dioxide, water, and chloride.


Effects of sodium arsenite on fetal development.


Polychlorinated terphenyls in the environment.


Chlorinated hydrocarbon residues in marine animals of southern California.


Similarity of a polychlorinated biphenyl (Aroclor 1254) and DDE in rate of elimination from cows.


Improved procedures for the determination of oxytetracycline in milk, milk products; chicken muscle, liver; and eggs.


Discrimination between PCB and DDT residues by a gas chromatographic-mass spectrometric technique.


Polychlorinated biphenyls: photolysis of 3, 4, 3', 4'-tetrachlorobiphenyl and 4, 4'-dichlorobiphenyl in solution.


Acute intraperitoneal toxicity of DDT and PCB's in mice using two solvents.


The malignant transformation as a metabolic steady state transition: the possible significance of the phasing of enzyme synthesis and related aspects.


An investigation of the aetiology of the 1966 outbreak of febrile illness in Jabalpur, Madhya Pradesh.


Preliminary studies of body potassium in normal adults by whole body counter.


Vital signs in progressive intracranial hypertension: an experimental study.


Susceptibility of N. gonorrhoeae in vitro to antibiotics (penicillin, oxytetracycline, chloramphenicol and rifampicin).


Use of isotopic abundance ratios in identification of polychlorinated biphenyls by mass spectrometry.


Inhibition of lipid biosynthesis by p-chlorophenoxy-isobutyrate (CPIB) in Tetrahymena pyriformis.


Antigenic properties of some insects involved in food contamination.


Studies on ion transport in cells of photosynthetic bacteria. I. The key role of anions in determining the direction and magnitude of hydrogen ion flux.


Studies on ion transport in cells of photosynthetic bacteria. II. Analysis of reversed hydrogen ion change.


Studies on ion transport in cells of photosynthetic bacteria. 3. The influence of uncouplers on hydrogen ion change.


The characteristic action patterns of crystalline bacterial saccharifying alpha-amylase independent of its microheterogeneity.


Competition between Pi and pH indicators in photosynthetic ATP formation in chromatophores of Rhodospirillum rubrum.


Effects of ventral putamen lesions on discrimination learning by monkeys.


Carrier rate of Neisseria meningitidis and the occurrence of sulfonamide resistant strains in Lebanon.


A study of passive potassium efflux from human red blood cells using ion-specific electrodes.


The penetration of water into the epithelium of toad urinary bladder and its modification by oxytocin.


An analysis of the relationship between the current and potential generated by a quantum of acetylcholine in muscle fibers without transverse tubules.


A new myxosporidan in red and golden shiners.


Effect of antiserum on maltase from intestinal microvillous membranes of Ascaris suum.


An evaluation of the direct agglutination test for Chagas' disease.


Significance of intimal arterial changes in non-human vertebrates.


Physiologic and clinical actions of nitroglycerin.


Diagnosis of angina pectoris at the present time.


Diabetes mellitus. New developments.

Diabetes mellitus. New developments.


The etiology and pathogenesis of osteoarthrosis: a review.


Long-term toxicity study of methylmercuric chloride in monkeys (first report).


Competitive inhibition between oxytocin and luteinizing hormone-releasing factor (LRF) for the same enzyme system in the rat hypothalamus.


Some factors influencing TRH bioassay in mice.


Rapid estimation of human chorionic somatomammotrophin (HCS) by haemagglutination inhibition and comparison of results with radioimmunoassay.


Influence of oophorectomy, luteectomy, foetal death and dexamethasone on the peripheral plasma levels of oestrogens and progesterone in the pregnant Macaca mulatta.


Nitrogen-15 studies of erythropoiesis during recovery from marasmus.


Vitamins A and E nutrition via intramuscular or oral route.


Effects of protein-calorie malnutrition on the jejunal mucosa of tetracycline-treated pigs.


Cerebral arterial occlusion and cluster headaches in neurofibromatosis.


Red blood cell beta-aminolevulinic acid dehydrase activity. An index of body lead burden.


Letter: Pseudomonas infections.

Letter: Pseudomonas infections.


Infant and child enteritis-malabsorption-malnutrition: the potential of limited studies with low-dose antibiotic feeding.


Letter: What is meant by "bimonthly"?

Letter: What is meant by "bimonthly"?


Plasma and red cell water and elements in protein-calorie malnutrition.


Immunofluorescent studies in mucous membrane pemphigoid.


Bacteraemia as a complication of subclavian vein catheterisation.


Letter: Gold treatment of pemphigus.

Letter: Gold treatment of pemphigus.


Editorial: Hereditary eye disease and prenatal diagnosis.


Laboratory diagnosis of increased lead absorption.


Electrophysiological and psychophysical findings in Hunter syndrome.


Column chromatographic separation of polychlorinated biphenyls from chlorinated hydrocarbon pesticides, and their subsequent gas chromatographic quantitation in terms of derivatives.


A general method for the preparation of tritiated polychlorobiphenyls of high specific activity: 2,2',5,5'-tetrachlorobiphenyl-(3H) and 2,2',4,4',5,5'-hexachlorobiphenyl-(3H).


Separation of aldrin from Aroclor 1254.

Separation of aldrin from Aroclor 1254.


Mirex residues in wild populations of the edible red crawfish (Procambarus clarki).


PCB residues in Atlantic zooplankton.

PCB residues in Atlantic zooplankton.


The extended care philosophy: A must for the long-term patient.


Tumor-associated antigen(s) from granulosa cell carcinomas of the ovary.


Factors influencing admission and discharge from a hospital for the chronic sick in Israel.


A survey of pulmonary tuberculosis in the elderly in North Staffordshire.


Treatment of streptococcal pharyngitis with different antibiotic regimens.


Serum levels of C1q, C1r and C1s in normal and pathologic sera.


The changing pattern of geriatric care.  A 20-year survey report from the West Cornwall clinical area.


Collaborative study of the recovery and gas chromatographic quantitation of polychlorinated biphenyls in chicken fat and polychlorinated biphenyl-DDT combinations in fish.


Polychlorinated biphenyls and polychlorinated terphenyls in Canadian food packaging materials.


A serum component related to nonimmunoglobulin amyloid protein AS, a possible precursor of the fibrils. A nonimmunoglobulin protein with the molecular weight of 9,145 (protein AS) has been shown to be a principal component of the amyloid fibrils in different clinical types of amyloidosis. A protein component, antigenically closely related to protein AS, was detected in human sera. The protein AS-related component (protein ASC) was found in the sera of many groups of patients, including 48 out of 55 patients with various clinical types of amyloidosis. No structural relationship of protein ASC to the plasma component of amyloid was found. Protein ASC was also present with high frequency in the serum of diseases known to be frequently complicated by amyloidosis. In some cases, ASC was found in the sera of patients 2-3 yr before the diagnosis of amyloidosis was established. Protein ASC was also frequently found in hypogammaglobulinemia. Among normal individuals, protein ASC was seldom detected in the serum by our techniques, but there was a noticeable increase with age and during pregnancy. Moreover, a more sensitive technique, immunoelectro-osmophoresis, revealed protein ASC in a higher number of sera from both patients and normal controls. Thus protein ASC was suggested to be a normal serum constituent, usually present only in minor quantities. Under certain conditions, protein ASC increases considerably in serum, and may in such instances act as a precursor for the deposition of amyloid fibrils in the tissues.


Disturbance of serum viscosity in diabetes mellitus. The serum viscosity of diabetic patients has been found to be increased. The elevation averaged 8% above healthy subjects and 6% above nondiabetic patients. The serum viscosity elevation was greater when diabetic sequelae associated with microangiopathy were present. No relation of serum viscosity to age, sex, obesity, duration of disease, or type of treatment was demonstrated. Serum total protein and glucose levels were found to be correlated with serum viscosity, and increases in their serum concentrations were observed in diabetes. Analysis demonstrated that their elevation did not explain either the viscosity increase or the difference in viscosity between diabetics with and without sequelae.Intrinsic viscosity, abbreviated [eta], is a concentration-independent solute property related to molecular shape. [eta] was found to be 7% higher in diabetic than in normal serum. The [eta] difference accounted for at least half of the serum viscosity elevation. The rest of the increase was due to increased serum protein level and increased nonprotein solids, presumably glucose and lipid. Associated with increased [eta] was a decline in albumin: globulin ratio and elevation of the acute phase reactant proteins, alpha(1)-acid glycoprotein, alpha(1)-antitrypsin, haptoglobin, and ceruloplasmin. Studies comparing diabetic and normal serum fractionated by using 21.5% sodium sulfate showed that changes in [eta] were attributable to changes in serum protein composition rather than an inherent qualitative disturbance of protein present in one of the fractions. Since serum viscosity is elevated in early diabetes, it may be a part of the metabolic disturbance of diabetes and could play a role in the development of diabetic microangiopathy.


Local and systemic cell-mediated immunity after immunization of guinea pigs with live or killed m. tuberculosis by various routes.


The isolation and characterization of an anti-sheep red blood cell antibody having limited heterogeneity.


Antigenic studies of J chain.

Antigenic studies of J chain.


Characterization of the Fv fragment isolated from a human immunoglobulin M.


Requirement for bone marrow-derived cells in resistance to Listeria.


The cooperative health statistics system.


Survey instrument to study institutional care services for convalescent and long-term patients.


Changes in antigenic properties of human C3 upon activation and conversion by trypsin.


Effect of polyadenine: polyuridine on brucellosis in conventional and congenitally athymic mice.


Mucosal and glandular distribution of immunoglobulin components: differential localization of free and bound SC in secretory epithelial cells.


Immunoglobulins with multiple binding functions. II. The use of nylon-polyserine whisker discs in screening myeloma immunoglobulins for binding activity.


Increased clearance of antipyrine and d-propranolol after phenobarbital treatment in the monkey. Relative contributions of enzyme induction and increased hepatic blood flow. The effects of phenobarbital treatment for 12 days on the regional distribution of blood flow and on the disposition of two model drugs, antipyrine and d-propranolol, have been determined in six unanesthetized rhesus monkeys. Phenobarbital significantly increased total hepatic blood flow from 179+/-15 to 239+/-27 ml/min. Liver weight was increased to a similar degree (34%) in phenobarbital-treated animals as compared to control monkeys. The clearance of both antipyrine and d-propranolol was increased and the half-life decreased significantly by phenobarbital. Analysis of the data by a perfusion-limited pharmacokinetic model showed that the changes in antipyrine clearance were due almost entirely to enzyme induction. On the other hand, with d-propranolol, the increase in liver blood flow contributed as much to the enhanced clearance as did the stimulation of drug metabolism. The mechanism by which phenobarbital produces the frequently observed increase in drug clearance, therefore, depends upon the initial clearance value of the drug. For low clearance drugs like antipyrine, clearance changes occur largely as a result of enzyme induction. With higher clearance drugs, the effects of increased hepatic blood flow become progressively more important the greater the initial clearance value.


Studies of cellular immunity in Chagas disease: effect of glutaraldehyde-treated specific antigen on inhibition of leukocyte migration.


Medial occipital projections to the nucleus of Darkschewitsch of rhesus monkey.


Trouble-free microbiologic serum and red cell folate assays.


Lactic dehydrogenase activity in the hair follicles of the stump-tailed macaque (Macaca speciosa).


Carbaryl residues in bees, honey, and bee bread following exposure to carbaryl via the food supply.


PCB levels in egg yolks associated with embryonic mortality and deformity of hatched chicks.


Spontaneous awake EEG patterns in three lines of primate evolution.


Interocular transfer of mirror image discriminations by chiasm-sectioned monkeys.


Loss of social group affinity following prefrontal lesions in free-ranging macaques.


Concerning the influence of experimental conditions on social interactions initiated by telestimulation in squirrel monkey groups.


Interocular reversal of preference for lateral mirror-image shapes by the monkey with sectioned optic chiasm.


On the sensitivity of pseudomonas and proteus to the combination of trimethoprim and sulphamethoxazole.


Control of catabolism of mitochondria in oncogenesis. I. Increase of hepatic mitochondrial population during feeding inactive or marginally active amino azo dyes: absence of de novo biosynthesis.


Follow-up of a group of workers exposed to dusts containing derivatives of Bacillus subtilis.


Clinical and immunological responses to enzymes of Bacillus subtilis in factory workers and consumers.


Farmer's lung: a comparison of simple diagnostic techniques and antigen preparation in human and bovine disease.


The anatomy of the mitochondrial DNA. The localization of the heat-induced and RNAase-induced scissions in the phosphodiester backbones.


Mutants of Bacillus subtilis blocked in acetoin reductase.


Comparison of the suppressive effect of thymus cells and the suppression by neonatal application of antigen.


Active subunits of transaldolase bound to sepharose.


Antibodies specific for human T lymphocytes in cold agglutinin and lymphocytotoxic sera.


Seasonal and hormonally induced changes in the serum level of the precursor protein vitellogenin in relation to ovarian vitellogenic growth in the toad Bufo bufo bufo (L.).


The effect of lesions in the centromedian nucleus of the thalamus on the monkey's performance in delayed alternation and object reversal tasks.


Interrelationships in the regulation of TSH and prolactin secretion in man: effects of L-dopa, TRH and thyroid hormone in various combinations.


The effect of an ethanol-extractable thyroxine binding substance in plasma on the measurement of thyroxine by competitive binding.


Late hemispherectomy complications. Unilateral hydrocephalus from aqueductal obstruction.


Participation of medial pontine reticular formation in eye movement generation in monkey.


Precentral and postcentral cortical activity in association with visually triggered movement.


Responses of primate spinothalamic tract neurons to natural stimulation of hindlimb.


Progesterone-binding protein in human myometrium. Influence of metal ions on binding.


Scanning of the apical pole of distal tubular cells under differing acid-base conditions.


Muscle AMP aminohydrolase. X. Effect of anions on the kinetic and regulatory properties of the rat muscle enzyme.


Zen macrobiotic dietary problems in infancy.


Serum aldosterone concentrations related to sodium balance in the newborn infant.


Catheter complications in total parenteral nutrition. A prospective study of 200 consecutive patients.


Visceral neurofibromatosis and hypertension in childhood.


Neonatal gonococcal arthritis: three cases and review of the literature.


Chelation therapy in children with subclinical plumbism.


Acetylcholinesterase from a non-membrane source (Bungarus fasciatus venom).


Alcoholism and animals.

Alcoholism and animals.


Smallpox vaccination and its consequences: first experiences with the highly attenuated smallpox vaccine "MVA".


Calcium-induced extrusion of secretory granules (exocytosis) in mast cells exposed to 48-80 or the ionophores A-23187 and X-537A. Isolated peritoneal mast cells from rats were observed, by phase contrast microscopy, to extrude secretory granules when exposed to 48/80 or the ionophores A-23187 and X-537A, which are known to facilitate transmembrane fluxes of calcium. These effects were abolished when the cells were treated with EDTA and suspended in a Ca-free environment. Ca-deprived cells exposed to any one of the three drugs promptly extruded granules when calcium, but not magnesium, was added to the incubation medium. Such Ca-evoked or Ca-dependent responses persisted when Na was omitted from the incubation medium and replaced with sucrose, choline, or K. The responses thus seem independent of possible shifts in the alkali metal ions. The results are considered support for the view that Ca influx mediates stimulus-secretion coupling and does so by initiating exocytosis.


A comparison of bovine prothrombin, factor IX (Christmas factor), and factor X (Stuart factor). A comparison has been made of the electrophoretic behavior, chemical composition, amino-terminal sequence, and immunological properties of bovine prothrombin, factor IX (Christmas factor), and factor X (Stuart factor). Some immunological crossreactivity was found between the antibody to prothrombin and factor X although prothrombin and factor X differ substantially in amino-acid and carbohydrate composition. Considerable amino-acid sequence homology was found in the amino-terminal portion of prothrombin, factor IX, and the light chain of factor X. These data provide further evidence to support the hypothesis that at least three of the vitamin K-dependent clotting factors have evolved from a common ancestral gene.


In vitro synthesis of ribosomal proteins directed by Escherichia coli DNA. In vitro synthesis of a number of E. coli 30S ribosomal proteins has been demonstrated in a cell-free system consisting of ribosomes, initiation factors, RNA polymerase, a fraction containing soluble enzymes and factors, and E. coli DNA. DNA-dependent synthesis of the following 30S proteins has been demonstrated: S4, S5, S7, S8, S9, S10, S13, S14, S16, S19, and S20.


Chemotactic activity in dialyzable transfer factor. Dialyzable transfer factor from human leukocytes was found to be strongly chemotactic for granulocytes and weakly chemotactic for monocytes in vitro. Chemotactic properties were also demonstrated in vivo in rhesus monkey skin. Initial purification of the dialyzable transfer factor by Sephadex G-25 chromatography revealed multiple fractions containing material with 255-nm absorbance. The fractions containing chemotactic activity were also capable of transferring delayed hypersensitivity to rhesus monkeys. This chemotactic material has an apparent molecular weight of 5000 daltons or less and was not inactivated by goat antibody to components C3 or C5 of human complement; chemotactic activity was lost after storage for two weeks at 4 degrees and the activity of two of three preparations was decreased by heating for 30 min at 56 degrees . This previously undescribed chemotactic activity of dialyzable transfer factor may have significance in relation to cell-mediated immune responses.


Immunostimulation with bacterial phospholipid extracts. Injection of bacterial phospholipid extracts (EBP) into mice increased their resistance towards a Listeria monocytogenes infection. The blood clearance of virulent Salmonella typhimurium was enhanced and the degree of clearance correlated with the dose of extract injected. The multiplication of Listeria monocytogenes in spleen and liver of mice was inhibited and this inhibition was also correlated with the amount of extract injected. The absence of apparent toxicity in mice, of splenoand hepatomegaly, and of lymphoid hyperplasia, distinguish this immunostimulant from other known bacterial stimulants of host resistance to infection.


Toxigenic algae.

Toxigenic algae.


Role of prostaglandins in estrogen-induced uterine hyperemia.


Inability of prostaglandin synthesis inhibitors to affect adipose tissue lipolysis.


Specific binding sites in the rabbit kidney for prostaglandin A.


Increased forearm venous compliance during exercise with nitroglycerin.


The estimation of molecular weight of tonofilaments by gel-filtration in 6M guanidine hydrochloride and by sodium dodecylsulfate acrylamide gel electrophoresis.


Insulin deficiency and hyperosmolar coma.


Gonococcal infection. Clinical, epidemiologic and laboratory perspectives.


Thyrotropin-releasing hormone.

Thyrotropin-releasing hormone.


A contribution to the solitary ciliogenesis.


Polychlorinated biphenyl-induced decrease in liver vitamin A in Japanese quail and rats.


Contribution to study of the arterial supply of the gastrointestinal tract of the toad (Bufo bufo).


Macroglobulin-producing histiocytic lymphoma of hepatic origin.


Residues of mirex and certain other chlorinated hydrocarbon insecticides in beef fat--1971.


Perforated jejunal diverticulum diagnosed as acute appendicitis.


Osteochondritis dissecans of the talus.

Osteochondritis dissecans of the talus.


Neurological sequelae of decompression in supersonic transport aircraft.


Specific impairment of host defense for pseudomonas infection in mice by a burn toxin derived from thermally altered human skin.


The unilateral engram.

The unilateral engram. It is proposed that the corpus callosum has a major role in the processes of memory; first, by providing access by each hemisphere to memory traces stored in the other, and second, by controlling the formation of memory traces in such a way that they are laid down in only one hemisphere instead of in both. This dual mechanism would have the effect of doubling the mnemonic storage capacity of the brain. Evidence in favor of this hypothesis in briefly reviewed. It is also supported by experiments using electrical excitation of the striate cortex as a conditional stimulus in macaques, and by experiments on maze behavior in the m e species. Experiments with the anterior commissure, on the other hand, show that it, in contrast to the splenium of the corpus callosum, can actually transfer an engram from one hemisphere to the other. Finally, it is shown that the splenium provides an effective path of communication between the central visual system in one hemisphere and the amygdala in the other.


Some characteristics of self-stimulation behavior of dogs. Self-stimulation was studied in dogs chronically implanted with electrodes in different points within the basal forebrain. The animals exhibiting pure self-rewarding behavior were defined as "optimal" and "good self-stimulators", whereas those with concomitant aversive phenomena were incorporated into the third category called "self-stimulation-withdrawal". In "optimal self-stimulators" a remarkable resistance of the response to extinction was noted. A strong negative attitude toward food was found in four dogs upon stimulation of the self-rewarding loci. Penile erection accompanied self-stimulation in two animals. Sniffing at first always followed incentive brain stimulation, but later it appeared at the beginning of each experiment and/or preceded the bouts of pressing. A rise of hypothalamic temperature was noted in mast of the animals. In some cases this was equal to or exceeded 1 degree Celsius. The temperature increase was accompanied by intense panting between the bouts. Seizures appeared locally as contractions of masticatory muscles and sometimes developed into a generalized fit. Anatomically the "reward area" in the dog extends from the septum and the preoptic area to the mammillary bodies and reaches laterally to the internal capsule.


Analysis of the polychlorinated biphenyl problem. Application of gas chromatography-mass spectrometry with computer controlled repetitive data acquisition from selected specific lons.


Demonstration of nuclear and cytoplasmic fluorescence in brain tissues of schizophrenic and nonschizophrenic patients.


Health services for foster children: an evaluation of agency programs.


Clinical and social aspects of a non-geriatric long-stay psychiatric unit.


Antidepressant effect of thyrotropin-releasing hormone (TRH) and the plasma thyrotropin levels in depression.


L-glutamate decarboxylase and choline acetyltransferase activity in the substantia nigra and the striatum after surgical interruption of the strio-nigral fibres of the baboon.


Timing of meiotic crossing-over in Drosophila melanogaster.


Effects of cadmium on development time and prepupal puffing pattern of Drosophila melanogaster.


A chlorine-free embedding medium for use in x-ray analytical electron microscope localisation of chloride in biological tissues.


Gonococcal vaccine studies in Inuvik.

Gonococcal vaccine studies in Inuvik.


Maridomycin, a new macrolide antibiotic. I. Taxonomy and fermentation.


Functional radioisotope investigations in chronic nephritis.


The effects of different preparative procedures on the ultrastructure of the hamster ovary. I. Effects of various fixative solutions on ovarian oocytes and their granulosa cells.


Aminoglycoside antibiotics. IV. BU-1709 E1 and E2, new aminoglycoside antibiotics related to the butirosins.


Study on in vitro activity of the antibiotics tobramycin and gentamicin against Pseudomonas aeruginosa clinical strains.


Isolation and characterization of the methionine antagonist L-2-amino-4-methoxy-trans-3-butenoic acid from Pseudomonas aeruginosa grown on n-paraffin.


Bias functions and operating characteristics of rats discriminating auditory stimuli. Rats were trained to discriminate between two bursts of random noise that differed in intensity. In a two-lever, discrete-trial procedure, correct responses were reinforced with brain stimulation, and incorrect responses produced timeout. Responding was studied as a function of the decibel difference between the stimuli, the probabilities of presenting the stimuli, the relative duration of timeout consequent upon the two types of incorrect responses, and the absolute duration of timeout consequent upon incorrect responses. The results showed that the distribution of responses between the two levers depended upon the stimulus probabilities, but were independent of either the absolute or relative durations of timeout. When the stimulus probabilities were varied, the response probabilities did not match the stimulus probabilities; when the relative durations of timeout were varied, the animals did not obtain the maximum rate of reinforcement per unit time. Instead, the animals distributed their responses so as to obtain the maximum number of reinforcements at each level of discrimination. In addition, the level of discrimination increased as a function of the decibel difference between the stimuli.


Monkeys agree-noise is upsetting.

Monkeys agree-noise is upsetting.


Basic studies on 3', 4'-dideoxykanamycin B.


Role of a myocardial depressant factor in shock states.


Thermal degradation of some parenteral solutions of reserpine. 2. Effects of ionic strength, dielectric constant, inert gas and stabilizers.


The case history of monkey no. 109: first experimentally produced vertical gaze palsy.


Potential changes associated with eye movements in the thalamus, visual cortex and reticular formation.


Birth order and parental experience in monkeys and man.


Biochemistry of natural proteinase inhibitors.


Biological control of mosquito larvae.

Biological control of mosquito larvae.


Courtship behavior in Drosophila.

Courtship behavior in Drosophila.


Animal learning. Visceral and autonomic conditioning.


Spatial vision.

Spatial vision.


Somesthesis.

Somesthesis.


Brain function: changing ideas on the role of sensory, motor, and association cortex in behavior.


Coccidioidomycosis in a baboon recently imported from California.


Intracellular location of newly synthesized growth hormone.


On the macromolecular composition of the jelly coat from Strongylocentrotus purpuratus eggs.


Macrophage spreading in vitro. II. Manganese and other metals as inducers or as co-factors for induced spreading.


A radioimmunoassay for myosin in cultured skeletal muscle cells.


Effect of beta-mercaptoethanol on the binary fission in Spirotomum ambiguum Mull-Ehrbg.


The role of wounds in the epidemiology of nosocomial infections due to Pseudomonas aeruginosa.


Gene control of soluble protein levels during development of abnormal abdomen in Drosophila.


Editorial: Trimethoprim-sulfamethoxazole (TMP-SMZ)--better late than never.


A study on the effects of ibuprofen on the metabolism of antipyrine in man.


Letter: Intal in refractory asthmatic paediatric cases.


Cannabis and reverse tolerance: pharmacological entity or psychological phenomenon?


Comparable bactericidal activity of two chemosterilizers.


Effect of microbial interactions on in vitro plaque formation by Streptococcus mutans.


Radiographic cephalometric method of assessment of craniofacial growth in monkeys.


Electron probe microanalysis of human enamel microstructure.


An evaluation of EDTA in endodontics.

An evaluation of EDTA in endodontics.


Medicolegal blood grouping tests (parentage exclusion tests).


Clinical microbiology of unusual Pseudomonas species.


Immunodiffusion studies on some Proteus strains.


The significance of Australia-antibody in blood donors. Study of liver histology in donors and post-transfusion hepatitis in recipients.


New electrophoretic information on the surface composition of the rat erythrocyte.


Bacillus megaterium, KM beta-galactosidase: purification by affinity chromatography and characterization of the active species.


Bacteriophage-coded thymidylate synthetase. Evidence that the T4 enzyme is a capsid protein.


Alpha-L-iduronidase activity in cultured skin fibroblasts and amniotic fluid cells.


Computational methods in beam therapy planning.


EXTDOS 71. Revised and expanded version of EXTDOS, a program for treatment planning in external beam therapy.


A versatile system for three-dimensional radiation dose computation and display, RTP.


Program IRREG-calculation of dose from irregularly shaped radiation beams.


A computer program for optimised stationary beam treatment planning using score functions.


National Cancer Center radiotherapy computer system.


Relative performance of selected toxigenic and non-toxigenic isolates of Aspergillus flavus Link ex Fries on different culture media.


Uterine bleeding in rhesus monkeys after insertion of polyethylene and copper containing polyethylene intrauterine contraceptive devices. The effect of polyethylene devices, with or without copper, on the incidence of postinsertion bleeding episodes in rhesus monkeys is reported.  24 rhesus monkeys were divided into 4 groups.  Group 1 was fitted with the Lippes loop (No. 25).  Group 2 was fitted with the loop with 2 cm copper wire (.2 mm diam.) wound around it.  Group 3 was fitted with plain polyethylene T-device without copper, and Group 4 was fitted with copper-T (model TCu-200 L).  Vaginal smears of all of the monkeys were examined every 12 hours for microscopic bleeding.  1 of the animals of each group showing frank uterine bleeding was sacrificed.  The uterus was dissected out and processed for histological studies.  The results showed that the incidence of postinsertion bleeding in rhesus monkeys varied according to the design and material of the devices.  The severity of bleeding was markedly less with either the plain T-device or the T-device with copper, as compared to Lippes loop with or without this metal.  The copper containing Lippes loop caused less bleeding in so far as duration and flow were concerned than the loop without copper.  The severity of bleeding in animals fitted with plain T-device was less than that seen after insertion of the plain Lippes loop.  This suggests that the design of the device is a crucial factor in bleeding.  A comparison between plain T-devices and copper T-devices showed a beneficial action of copper in reducing the incidence of postinsertion bleeding.


Antigenic relationship between ruminant serum proteins.


Studies on antiviral property of fruiting Myxobacteria (Myxococcus virescens).


The primary structures of alpha and beta chains of adult hemoglobin of the Japanese monkey (Macata fuscata fuscata). Biochemical studies on hemoglobins and myoglobins. XI.


The primary structures of the alpha and beta polypeptide chains of adult hemoglobin of the Hanumam langur (Presbytis entellus).


Chemical modification of neutral subtilopeptidase by aromatic diazo compounds.


Letter: Pure-tone thresholds of the squirrel monkey (Saimiri sciureus).


Isolation and properties of mutants of the FP2 sex factor of Pseudomonas aeruginosa.


Gram-negative infections in the ICU.

Gram-negative infections in the ICU.


Studies related to penicillins. XI. Mechanism of degradation of benzylpenicillinic and phenoxymethylpenicillinic acid by mercury(II) acetate.


The genetic analysis of the direct and correlated response to selection for chaetae in Drosophila melanogaster.


Genetic and environmental control of a continuous trait, abnormal abdomen, in Drosophila melanogaster.


Genetic differences between populations of Drosophila melanogaster for a quantitative trait. I. Laboratory populations.


Genetic differences between populations of Drosophila melanogaster for a quantitative trait. II. Wild and laboratory populations.


Acute urethritis in male college students.


The effect of peroxidized arachidonic acid upon human platelet aggregation.


No one told me to.

No one told me to.


Evocation of a biphasic febrile response in the rhesus monkey by intracerebral injection of bacterial endotoxins.


Isozyme mutation rate in mutator stock of Drosophila melanogaster.


Intestinal microbial flora and proteolytic activity of intestinal contents.


Amniotic fluid, epinephrine, and norepinephrine. I. Comparison between the human and baboon.


Gonococcal conjunctivitis.

Gonococcal conjunctivitis.


Insulin response in patients receiving concentrated infusions of glucose and casein hydrolysate for complete parenteral nutrition.


The effects of short-term steroid therapy on lung bacterial clearance and survival in rats.


Total intravenous feeding of infants and children without use of a central venous catheter.


New techniques for long-term intravenous feeding: an artificial gut in 75 patients.


Letter: Intrapartum sedation of fetus.

Letter: Intrapartum sedation of fetus.


Studies on the growth of Thiobacillus ferrooxidans. II. Toxicity of uranium to growing cultures and tolerance conferred by mutation, other metal cations and EDTA.


Dihydrogenated alkaloids of ergot in geriatric patients: symptomatic treatment of cerebrovascular insufficiency.


Proceedings: Intravenous hyperalimentation in the treatment of inflammatory diseases of the bowel.


X-ray analysis of retinal photoreceptor structure.


An analysis of the absorption and circular dichroism of some visual pigments.


Ketamine and epilepsy.

Ketamine and epilepsy.


Serum immunoglobulin levels in women using intrauterine contraceptive devices.


Letter: Differentiation of coccidia in cats and transmission of toxoplasmosis.


Modulation of hypothalamic ICSS by concurrent limbic stimulation.


Congenital anterior urethral diverticulum in the male.


Geometric penumbra calculations for a gas target neutron source.


X radiation and sensitized lymphocyte migration.


Combined cytolytic effect of x irradiation and cell-mediated immune reactions on tumor cells in vitro.


Carcinoembryonic antigen in patients with squamous cell carcinoma of the cervix uteri and vulva.


Control of the differentiated state in somatic cell hybrids.


Mapping of behavior in Drosophila mosaics.


Genetics of sperm development in Drosophila.


Determination of regional myocardial blood flow (MBF) in patients with anginal pain.


Cobalt 60 treatment field verification by xeroradiography.


Serial scintigrams in the course of ectopic bone formation in paraplegic patients.


Oxygen cycles and metabolic autoregulation.


Percentage depth dose for high energy x-ray beams in radiotherapy.


The effect of graded hypocapnia and hypercapnia on regional cerebral blood flow and cerebral vessel caliber in the rhesus monkey: study of cerebral hemodynamics following subarachnoid hemorrhage and traumatic internal carotid spasm.


Reduced sulphation factor in undernourished children.


Serum C3 levels in acute glomerulonephritis and postnephritic children.


Massive gastrointestinal bleeding. A panel by correspondence.


Adrenal steroids in the treatment of malignant hematologic disease.


Human prolactin in clinical endocrinology: the impact of radioimmunoassays.


Megavoltage radiation therapy for supraglottic carcinoma. Results of treatment.


Radiation dosimetry for large, irregularly-shaped fields in the treatment of acute lymphocytic leukemia.


Effect of various atomic number absorbers on skin dose for 10-MeV x rays.


Pseudomonas osteomyelitis. Radiologic features.


Chronic overdrive pacing in the control of refractory ventricular arrhythmias.


Letter: Bacillus cereus endocarditis in an addict.


Phase IV final health regulations. Commentary and background material as provided by the Cost of Living Council.


Fluorescent antibody studies in malignant melanoma. Sera from 57 patients with malignant melanoma and 39 control patients were tested by immunofluorescence techniques against 6 melanoma cell lines. Thirty-two per cent of tests with sera from melanoma patients showed fluorescence with these cell lines whereas only 17% of tests with control sera were positive. Reactions occurred in 21% of tests with sera from patients with primary melanoma compared with 40% with secondary melanomata and 54% with "cured" melanomata. The cell lines varied in antigenicity but this did not correlate with either pigmentation or length of time in culture. The cell lines which were most reactive with sera from melanoma patients were also most reactive with control sera.


Chronic prostatitis--an Australian view.


Studies on carcinogenic azo dyes. 3. Retention of tritium during enzymatic hydroxylation of 3'-methyl-4-dimethylaminoazobenzene(4'-3H or 5'-3H) or chemical substitutions of the related compound, and the effect of the repeated administration of this azo dye on the retention of tritium.


Ocular hypertensive response to alkali burns in the monkey.


An apparatus for the measurement of lipoxygenase activity and pentane production.


Letter: The probable identity of bacilysin and tetaine.


The use of blood.

The use of blood.


Studies on the antitumor activity of an alazopeptin isolated from a new strain of Streptomyces.


Letter: Fusaric acid derivatives: the effect on dopamine beta-hydroxylase.


The effect of sea star coelomocyte extract on cell-mediated resistance to Listeria monocytogenes in mice. Mice treated with sea star factor (SSF), a protein extracted from sea star coelomocytes, became highly susceptible to infection with a normally sublethal dose of Listeria monocytogenes. This was in contrast to the expected result of increased resistance originally postulated because of the macrophage-activating properties of SSF. Enhanced susceptibility was seen when SSF was given from 96 h before to 48 h after infection with Listeria. Mice pretreated with SSF failed to develop immunity to Listeria when given a dose of organisms capable of immunizing nontreated mice. Treatment of immune mice with SSF did not alter their immune status. In addition, incubation of immune lymphocytes with SSF in vitro did not alter their ability to adoptively transfer immunity to normal recipients. Immune lymphocytes treated with SSF and then incubated with anti-SSF and C did, however, lose the ability to transfer immunity. These results suggest that SSF enhances infection by binding to T lymphocytes, inhibiting their replication upon contact with Listeria antigen and thus preventing the generation of a population of sensitized lymphocytes capable of effecting anti-Listeria immunity.


Synergism between ecologic and immunologic control mechanisms of intestinal flora.


Resistance of Neisseria gonorrhoeae to phagocytosis: relationship to colonial morphology and surface pili.


New drugs--no. 14. Glibenclamide. Mechanism of action.


Pyelonephritis. XVII. Comparison of combinations of vancomycin, ampicillin, streptomycin, and gentamicin in the treatment of enterococcal infection in rats.


The value of radiotherapy in the management of malignant melanoma of the nasal cavity.


Auto-immunity in nasal allergy.

Auto-immunity in nasal allergy.


Gonococcal ophthalmia neonatorum. Relationship of time of infection to relevant control measures.


Total parenteral nutrition and Crohn's disease.


Mechanisms of carbohydrate-induced hypertriglyceridemia.


Tuberculosis in Acukland.

Tuberculosis in Acukland.


Intensive monitoring for adverse drug effects in patients discharged from acute medical wards.


Cytological investigation of crossovers induced by irradiation in males of Drosophila melanogaster.


Study of the mutation process induced by N-nitrosomethylurea at various stages of spermatogenesis of Drosophila melanogaster. Fertility, crossing-over, and recessive sex-linked lethal mutations.


Investigation of the mutagenic activity of phenothiazines and other drug preparations.


New boron-induced puffs and modificational localization of a gene.


Study of the competitive capacity of Drosophila melanogaster individuals heterozygous for radioinduced mutations.


Hemochromatosis presenting as diabetic ketoacidosis with extreme hyperglycemia.


Studies on tetrapeptides related to thyrotropin releasing hormone and luteinizing hormone releasing hormone.


The effect of anti-inflammatory drugs on the aggregation and adhesiveness of platelets, red cells and leukocytes.


Status and prospects of research in hereditary deafness.


Longitudinal differentiation of chromosomes.


Localization of gene function.

Localization of gene function.


Dosage compensation in Drosophila.

Dosage compensation in Drosophila.


Recombination deficient mutants of E. coli and other bacteria.


[Studies on the present state of resistance to framycetin among strains of staphylococci, enterobacteriaceae and pseudomonas].


Levodopa in the treatment of arterial insufficiency of the lower limbs. Clinical and rheographic trial.


Unprimed glucan biosynthesis by a particulate ADP-glucose-glucan glucosyl transferase from an Escherichia coli mutant and its stimulation by a protein factor.


Methylation of 4-thio-2'-deoxyuridylate by thymidylate synthetase.


Rat serum albumin biosynthesis: evidence for a precursor.


Clearance rate of exogenous 3H-thymidine from the plasma of pregnant rhesus monkeys.


Beta trace protein. Purification and urinary excretion studies in selected diseases.


Quantitative determination of IgE by single radial immunodiffusion. A comparison of three different methods for intensification of precipitates.


Significance of C-reactive protein binding by lecithin: a simplified procedure for CRP isolation.


Analytical characterization of the urinary proteins from sixty patients with monoclonal gammopathies.


A major role of the fifth component of complement (C5) in the opsonization of yeast particles. Partial dichotomy of function and immunochemical measurement.


Case report. Diverticulum of pharyngoesophageal junction.


Nutrition and cell growth.

Nutrition and cell growth.


Studies on the catalytic mechanism of a serine protease from Streptomyces griseus.


Competition in Drosophila. 3. A possible maternal effect.


Murein hydrolases in the envelope of Escherichia coli. Properties in situ and solubilization from the envelope.


Succession & stratification of organisms in sewage oxidation pond.


Cytological effect of versene on the production of dicentric bridges in spermatocytic chromosomes of grasshopper, Spathosternum prasiniferum Walker.


Characterisation & identification of two aerobic spore forming bacteria producing extracellular glutamic acid & valine respectively.


Staphylococcal enterotoxin synthesis during the exponential, transitional, and stationary growth phases. Small inocula (1 to 10 colony-forming units per ml of broth) of Staphylococcus aureus strains S-6, S-6R, and FRI-100 were employed to study growth and enterotoxin synthesis in 4% protein hydrolysate powder broths. For each strain, the exponential growth phase ended once the population approached 10(9) to 2 x 10(9) colony-forming units per ml. By that time, the concentrations of enterotoxins A and B reached the minimal level (1 to 2 mug/ml) at which the single gel diffusion tube method becomes applicable. By microslides and reverse passive hemagglutination, enterotoxins A and B were found to be synthesized during the exponential growth phase, but at different exponential rates.


Eperythrozoon coccoides. II. Effect on interferon production and role of humoral antibody in host resistance. Eperythrozoon coccoides was shown to inhibit the interferon response in mice only during the first 3 weeks after acute infection. By 6 weeks the production of interferon in response to Newcastle disease virus returned to a normal level. Antibody to E. coccoides could be demonstrated by three methods: (i) neutralization, (ii) indirect fluorescent antibody, and (iii) gel diffusion. The clearance of the parasitemia could be correlated with the appearance of circulating antibody. The critical role of humoral antibody was confirmed by evidence that immunosuppressive therapy prevented, in an infected animal, clearing of E. coccoides from the blood and converted a nonlethal infection to one with a 100% mortality. Administration of immune serum to the E. coccoides-infected, immunosuppressed animal, however, resulted in clearing of the parasitemia and prevention of death. Finally, a series of immunosuppressive regimens failed to exacerbate E. coccoides in animals after recovery from the acute phase of infection. E. coccoides may not be as important a laboratory contaminant as postulated originally.


Peptic digestion of streptococcal M protein. I. Effect of digestion at suboptimal pH upon the biological and immunochemical properties of purified M protein extracts. Purified streptococcal M protein vaccines often produce non-type-specific immunotoxic reactions in the skin or the blood of humans. In an attempt to free the type-specific M antigen (TSM) of such non-type-specific (NTSM) immunotoxic properties, purified M protein preparations were subjected to brief periods (5 to 10 min) of enzymatic digestion in dilute solutions of pepsin (20 mug/ml) at pH 5.0. Such peptic digestion abolished the ability of M protein to aggregate platelets in human platelet-rich plasma and to precipitate plasma fibrinogen. It greatly reduced (up to 16-fold) the capacity of M protein to react with the NTSM complement-fixing antibody that is normally present in human serum. In contrast, it had no effect upon the reactivity of the type-specific M antigen; the pepsin-treated M protein retained its ability to inhibit type-specific streptococcal opsonization by homologous M antibody, and, moreover, retained its ability to elicit type-specific opsonic antibody responses in rabbits.


Antibody-mediated inhibition of dextran-sucrose-induced agglutination of Streptococcus mutans. Immune sera to strains of Streptococcus mutans were found to inhibit agglutination of bacterial suspensions to which either high-molecular-weight dextran or sucrose was added. This inhibitory activity was shown to be mediated by antibody of the immunoglobulin G class. A semiquantitative assay was developed which demonstrated cross-inhibition of dextran/sucrose-induced agglutination among several strains of S. mutans. Antiserum to a partially purified glucosyltransferase was found to lack agglutination inhibition activity, consistent with the hypothesis that the dextran-binding antigen detected by the assay is immunologically distinct from the glucan-synthetic enzyme. A model for glucan synthesis and binding consistent with the reported data is described.


Development of a purified cholera toxoid. I. Purification of toxin. The enterotoxin from Vibrio cholerae is selectively concentrated from cell-free culture supernatant by co-precipitation with hexametaphosphate and is further purified by adsorption on aluminum hydroxide powder. The bulk of residual somatic antigen becomes insoluble upon lyophilization of the toxin preparation and is removed by centrifugation of the rehydrated material. Other contaminants are eliminated by treatment with activated carbon. Preparations of toxin, purified by this method, have been characterized by: (i) a single immunoprecipitin line against polyvalent antisera; (ii) homogeneity on acrylamide gels; (iii) specific activities on the order of 22 limit-of-bluing doses/mug; (iv) ultraviolet spectra characteristic of pure protein; and (v) overall yields on the order of 50%, irrespective of purification scale. Such preparations, however, have been shown to contain trace amounts of somatic antigen when they are intensively tested either for their ability to elevate serum vibriocidal antibody titers in immunized rabbits or for their ability to increase resistance of immunized mice to live vibrio challenge. In the latter test system, the level of residual somatic antigen per 50 mug of toxin (toxoid) antigen generally did not exceed 0.025% of the Division of Biological Standards reference vaccine, V. cholerae Inaba IN-12. Methods for elimination of this small amount of somatic antigen have been investigated and are discussed. The particular combination of purification steps which are presently described have been easily and reproducibly applied on a production scale to prepare gram amounts of toxin with a high degree of purity, even under a variety of initial conditions.


Development of a purified cholera toxoid. II. Preparation of a stable, antigenic toxoid by reaction of purified toxin with glutaraldehyde. Evidence is presented which confirms that cholera toxoids obtained by reaction of purified toxin with Formalin possess the ability to partially reactivate both in vivo and in vitro. At the same time, conditions are presented for the preparation of stable, antigenic cholera toxoids by reaction of purified toxin with glutaraldehyde. Treatment of purified cholera toxin with approximately 200 mol of glutaraldehyde per mol of toxin at pH 7.8 reproducibly resulted in the preparation of toxoids which: (i) possessed less than 20 bluing doses per 100 mug; (ii) did not reactivate in vivo or in vitro; (iii) precipitated with, and neutralized antitoxin; (iv) elevated prolonged serum antitoxin in immunized rabbits; (v) protected immunized guinea pigs against toxin skin challenge; and (vi) lent themselves to enhanced antigenicity by means of an in situ adjuvant system which may be suitable for man. Acrylamide gel electrophoresis and molecular sieve chromatography of a series of glutaraldehyde-derived toxoids suggested that the reaction products consisted of monomeric and polymeric species and that the proportion of higher-molecular-weight species was determined by the relative concentrations of toxin and glutaraldehyde. The results suggested a relationship between complete and irreversible elimination of toxicity and the formation of higher-molecular-weight toxoids.


Vi antigen from Salmonella typhosa and immunity against typhoid fever. I. Isolation and immunologic properties in animals. The role of Vi antigen in human immunity against typhoid fever has been debated for decades. Circumstantial evidence indicates that Vi antigen may play a role in pathogenicity and immunity. A Vi preparation was isolated from Salmonella typhosa, the causative organism in human typhoid fever, by a mild precipitation method. It was significantly more potent in animal studies than preparations from Citrobacter used in the past for human study and less toxic than conventional typhoid vaccines. With this antigen, the role of Vi antigen in human protection is now being investigated.


Relationship of antimicrobial cellular immunity to delayed hypersensitivity in Listeriosis. The relationship of antimicrobial cellular immunity to delayed hypersensitivity (DH) was studied in mice antigenically stimulated by living Listeria monocytogenes confined to diffusion chambers in peritoneal cavities or by subcutaneous inoculation of sublethal doses of the organism. Mice showed DH reactions when tested 6 days after inoculation, and reactions were positive for at least 90 days in some mice. DH also became established when the mice were stimulated by antigens diffusing from peritoneal chambers containing viable Listeria. Mice were categorized as DH positive or DH negative if they developed more or less than a 5% increase in foot volume 24 h after the injection of Listeria antigen. Some antigenically stimulated mice did not elicit the DH reaction. Consequently, the animals were arranged as immunized groups (DH positive and DH negative) and Listeria chamber implant groups (DH positive and DH negative). When challenged with L. monocytogenes, all four groups were significantly resistant as compared with controls. Thus, the in vivo tests for immunity and DH did not show direct correlation. The results suggested that antimicrobial cellular immunity can occur as a phenomenon independent of DH. Evidence for antimicrobial cellular immunity as the principle mechanism of resistance in murine listeriosis is discussed with consideration for possible heterogeneity of function by thymus-derived lymphocytes.


Feline immunoglobulins.

Feline immunoglobulins. Immunoglobulins (Ig) in feline sera and secretions were identified by immuno-electrophoresis and immunodiffusion with rabbit antisera prepared to feline IgG, IgA, IgM, and whole serum. Adult cat sera, colostral whey, postcolostral sera, tears, and nasal secretions contained IgG, IgA, and IgM. IgG was the only Ig identified in precolostral sera and cerebrospinal fluid. Milk, intestinal contents, pooled allantoic and amniotic fluids, and saliva from adult cats and urine from suckling kittens contained IgG and IgA. Ig were not detected in urine from adult cats. Bile was unique in that IgA and IgM were the predominant Ig.


Biological and physical properties of the suckling mouse cataract agent grown in chicken embryos. Experiments were carried out with the suckling mouse cataract agent (SMCA) in an attempt to better characterize the nature of this agent. Despite the fact that high titers of SMCA were found in the chorioallantoic fluid of inoculated chicken embryos, the agent did not replicate in chicken embryo epithelial cell cultures. SMCA did not interfere with Sindbis virus plaque formation and was not inhibited by chicken interferon or polyinosinic-polycytidylic acid. The agent was relatively resistant to high pH and osmotic shock but was inactivated by low pH. Electron micrographs of negatively stained particles concentrated and semipurified from infected chicken embryo chorioallantoic fluids revealed pleomorphic particles ranging from approximately 70 to 400 nm in diameter. SMCA was resistant to kanamycin, novobiocin, and sodium aurothiomalate.


Mechanism of adherence of Streptococcus mutans to smooth surfaces. II. Nature of the binding site and the adsorption of dextran-levan synthetase enzymes on the cell-wall surface of the streptococcus. The mechanism of adsorption of the Streptococcus mutans enzymes responsible for the synthesis of insoluble dextran-levan to the S. mutans cell-wall binding sites has been studied. Certain characteristics of these binding sites are presented. The adsorption of these enzymes to the cell surface occurred rapidly without the addition of a source of energy and over a pH range of 3 to 11. The adsorption was inhibited by soluble dextran, probably due to the strong affinity of the polymer to the enzyme. All other polymers and sugars studied showed little or no inhibition. The adsorption was also inhibited by antibody globulin to the a-d immunologically specific group antigen surface polysaccharide of S. mutans and by anti-dextran globulin. The inhibition by anti-a-d globulin is considered to be due to a restriction of access of enzyme to the binding site of the enzyme which may be located in close proximity to the group antigen. On the other hand, anti-dextran globulin appeared to directly inhibit the adsorption by covering the binding site. Dextranase destroyed the binding site and released glucose from the S. mutans cells. These data indicate that S. mutans grown in media containing glucose possesses a small amount of dextran on the cell surface, and that this dextran is, or is a part of, the binding site for enzymes which synthesize the insoluble dextran-levan polymer. Trypsin inhibited the synthesis of insoluble polysaccharide and the adherence of cells. It is not clear in this case that destruction of the binding sites occurred. These data present a partial explanation of the processes which may be concerned in the formation of dental plaque on the smooth surfaces of teeth.


Nonspecific inhibition of growth of intracellular Listeria monocytogenes by lymphocyte culture products. Supernatant fluids from mycobacteria-immune and mitogen-stimulated lymphocyte cultures were able to inhibit the intracellular growth of listeria in listeria-infected macrophages.


Maturation-inducing substance in starfishes.


Decreased susceptibility to Listeria monocytogenes in mice after infection with Trichinella spiralis. Mice challenged intravenously with Listeria monocytogenes at 7 or 21 days after Trichinella spiralis infection demonstrated a higher mean lethal dose and longer survival time than normal mice. This effect of helminthic infection was abrogated after 49 days.


Report on drugs, acidic and neutral nitrogenous organics.


Report on multiresidue methods (interlaboratory studies).


Fluorescence histochemistry of monoamine-containing cell bodies in the brain stem of the squirrel monkey (Saimiri sciureus). II. Catecholamine-containing groups.


Fluorescence histochemistry of monoamine-containing cell bodies in the brain stem of the squirrel monkey (Saimiri sciureus). 3. Serotonin-containing groups.


The morphology of retinal neurons of the owl monkey Aotes.


A clinical trial of a calculus-inhibitory dentifrice.


Total replacement of the elbow joint.

Total replacement of the elbow joint.


Porcine congenital infection due to Mycobacterium tuberculosis typus avium.


Effects of epinephrine and progesterone on the ocular hypertensive response to intravenous infusion of prostaglandin A2.


Pregnancy blockade by indomethacin, an inhibitor of prostaglandin synthesis: its reversal by prostaglandins and progesterone in mice.


Effect of metal-complexing agents on the oxygenase activity of sheep vesicular glands.


Arachidonic acid-induced human platelet aggregation and prostaglandin formation.


The embryo as a transplant.

The embryo as a transplant.


The selection of teaching-learning materials in health sciences education: report of a WHO study group.


Toxicological evaluation of certain food additives with a review of general principles and of specifications. Seventeenth report of the joint FAO-WHO Expert Committee on Food Additives.


Drug hydroxylation and glucuronidation in liver microsomes of phenobarbital-treated rats.


Investigation of the protease forming ability of Serratia marcescens and one of its mutant strains.


Repression of alkaline phosphatase synthesis by adenine, adenosine and adenine nucleotides in an adenine auxotroph of Bacillus anthracis.


Mycobacterium tuberculosis strains showing positive iron uptake.


A cryptic prophage carried by Bacillus cereus.


Serological relationship between Pseudomonas aeruginosa and Enterobacteriaceae. II. Relationship of Pseudomonas aeruginosa O antigens to Escherichia, Shigella, Proteus, Morganella, Rettgerella and Providencia.


Whole body counting of 32P-endotoxin elimination in endotoxin-tolerant and control mice.


Simple, sensitive spectrophotofluorometric method for hydrazine in plasma.


Life span and fine structural changes in oxygen-poisoned Drosophila melanogaster.


Application of the carbon cup atomisation technique in water analysis by atomic-absorption spectroscopy.


Synthesis of valinomycin by the phosphite method.


Effects of calcium and strontium on divalent ion content of refractive granules in Tetrahymena pyriformis.


Observations on alternative health strategies.


Role of specific sperm antigens in fertilization.


Blood bank inventory policies: a computer simulation.


Analysis of multiple sources of variation: comparison of three techniques.


Isolation of an acidic surface antigen from a conventional strain of Staphylococcus aureus. Staphylococcus aureus strain 7007, a prototype isolated from a hospital burn unit, was shown to exhibit a significant degree of resistance to ingestion by mouse polymorphonuclear leukocytes. An acidic surface antigen was isolated from strain 7007 by a combination of 10% trichloroacetic acid extraction, ion-exchange chromatography, and gel filtration. Chemical analysis indicated that the surface antigen consists of an unknown aminouronic acid and an amino sugar. Immunochemical analysis suggested that the 7007 antigen is a common feature of all the strains collected from the burn unit. No cross-reactivity was observed between the carbohydrate preparations of various hospital staphylococcal strains and the 7007 heteropolymer. These results suggested the possibility that the surface antigen of strain 7007 represents a strain- or type-specific antigen.


Effects of carbon dust inhalation on the cell-mediated immune response in mice. The effects of carbon dust inhalation on the bone marrow-derived (B) and thymus-derived (T) lymphocyte populations of spleen and mediastinal lymph node (MLN) cultures were examined. The concanavalin A (Con A)-responsive cell population (T cells) in the spleen was found to be depressed after 7 days of pre-exposure to carbon dust. However, this effect was transient, and after 14, 21, and 28 days of pre-exposure to carbon dust, the Con A-responsive cells exhibited a 30 to 40% enhancement over control group responses. Conversely, Con A-responsive cells in the pooled MLN cultures exhibited depression, ranging from 22 to 33% below control group values, after 7, 14, and 28 days of pre-exposure to carbon dust. The lipopolysaccharide (LPS)-responsive cell population (B cells) in the spleens of carbon-exposed mice was found not to differ significantly from control group values after all times of pre-exposure. LPS-responsive cells in the MLN cultures exhibited enhancement, ranging from 49 to 74% above control values, after 14, 21, and 28 days of pre-exposure to carbon dust. The ability of carbon spleen cell cultures from carbon-exposed mice to undergo antigen induced blast transformation after sensitization with Mycobacterium tuberculosis H37Ra was also determined. Mice exposed to carbon dust inhalation 2 weeks before and 3 weeks after aerosol or subcutaneous immunization exhibited significantly enhanced ratios of transformation upon culture of their spleen lymphocytes with purified protein derivative of tuberculin.


The familial nephrotic syndrome. II. A clinicopathological study.


Editorial: home dialysis or transplantation.


Stability of the rabbit immunogenic marker of RA 27-3 rubella vaccine virus after human passage. Rabbits were inoculated intravenously with "wild" rubella virus, RA 27/3 rubella vaccine virus, or rubella virus isolated from recipients of RA 27/3 vaccine. Rabbits receiving "wild" virus developed rubella hemagglutination inhibition antibody, and those receiving vaccine virus did not. One of the five reisolates tested produced a low transient antibody response in two of the five rabbits inoculated with this strain. The study indicates that the rabbit immunogenic marker after intravenous injection can be used to determine if a rubella virus isolated from a patient is of "wild" or vaccine origin. There was no significant change in the reduced immunogenicity characteristics of the RA 27/3 vaccine virus after human passage.


Pseudomonas aeruginosa exotoxin in mice: localization and effect on protein synthesis. We studied the fate of (125)I-labeled Pseudomonas aeruginosa (PA 103) exotoxin injected intravenously into mice and the effect of the exotoxin on the protein synthesis of various organs. After 2 h, only 5% of the injected label remained in the blood, in comparison with 30% of the injected control, consisting of [(125)I]bovine serum albumin. The highest concentration of radioactivity was consistently observed in the kidneys of toxin-treated mice. Lesser amounts of the label were found in the liver and the spleen. The heart, pancreas, lung, and brain showed very little uptake. Approximately 30% of the label (non-trichloroacetic acid precipitable) was recovered from the urine within 2 h after the injection. The behavior of [(125)I]toxoid was similar to that of the [(125)I]toxin. In contrast, in [(125)I]bovine serum albumin-treated mice, the label was uniformly distributed among the organs examined, and the concentration was low. After intravenous administration of the exotoxin, a 50% inhibition of protein synthesis was observed in the liver within 4 h, and virtually complete inhibition was observed shortly before the time of death. Kidney and spleen displayed slight reduction of protein synthesis at 2 to 4 h and approximately 50% reduction during the terminal stage. It appeared that the highest concentration of the toxin occurred in the kidney, where it was degraded, but its greatest toxic effect took place in the liver.


Immunological study of the heat-labile enterotoxins of Escherichia coli and Vibrio cholerae. Immunodiffusion experiments were conducted to associate a precipitin line with Escherichia coli heat-labile enterotoxin (LT). Wild strains of porcine and of human enteropathogenic E. coli as well as laboratory-derived enterotoxigenic variants of E. coli K-12 were used for LT antigen preparations. These were produced mainly by ultrafiltration and ammonium sulfate precipitation of broth culture supernatants. When antisera with anti-LT activity were reacted with antigen preparations from Ent(+) and Ent(-) variants of E. coli K-12, a line "a" was given by Ent(+) but not by Ent(-) preparations. Line "a" was removed by absorption of anti-LT serum with antigen preparation from an Ent(+)E. coli K-12, but was unaffected when the antigen preparation used to absorb the serum was from an Ent(-)E. coli K-12. A line identical to "a" was given by antigen preparations from wild strains of porcine enteropathogenic E. coli reacted with homologous or heterologous anti-LT sera. One human strain of enteropathogenic E. coli was shown to possess an antigen identical to that which gave rise to line "a." To demonstrate this line it was necessary to use high concentrations of gammaglobulin and high concentrations of the crude antigen preparations. LT preparations reacted with anticholera toxin to give a line "c," which showed a reaction of partial identity with line "b" produced by reaction of pure choleragenoid and anticholera toxin. Lines "a" and "c" gave reactions of identity.


Growth in children with renal disease with particular reference to the effects of calorie malnutrition: a review.


Mechanisms of immunity in typhus infections. IV. Failure of chicken embryo cells in culture to restrict growth of antibody-sensitized Rickettsia prowazeki. Rickettsia prowazeki, pretreated with typhus immune human serum, readily infects, and grows in, chicken embryo cells in culture. This finding is similar to those of previous studies which showed that typhus rickettsiae, pretreated with immune serum, grow in cells of the yolk sac of embryonated hen eggs and in the cells of the midgut of the human body louse. In contrast, identically treated typhus rickettsiae were destroyed by human macrophages in culture. Collectively, these observations seem to support an emerging concept that the fate of antibody-sensitized typhus rickettsiae depends upon the presence or absence of certain specialized properties of the host cell into which they gain entrance-nonphagocytic cells or "nonprofessional" phagocytic cells versus certain kinds of "professional" phagocytes. The phenomena involved probably have an important bearing on the mechanisms of the persisting infection and the nonsterile immunity which characterizes convalescence from typhus fever in man. They also form the basis for certain practical technical innovations in the laboratory.


The densities of colloidal iron hydroxide particles bound to microvilli and the spaces between them: studies on glutaraldehyde-fixed Ehrlich ascites tumour cells.


An examination into the phenomenon of granulation, in mu particles of stock 540 Paramecium aurelia.


Filamentous bacterial viruses. XI. Molecular architecture of the class II (Pf1, Xf) virion.


Hexachlorophene and the food and drug administration.


Changes in the scintigraphic aspects of liver after partial high-energy irradiation.


Review of the toxicity of hexachlorophene, including its neurotoxicity.


Hexachlorophene in perspective.

Hexachlorophene in perspective.


Cationic leukocyte antigen in urine of patients with chronic myelocytic leukaemia.


The significance of protein disulfide and sulfhydryl groups in prostaglandin action.


Measurement of prostaglandin f-2 alpha metabolites by radioimmunoassay.


An inhibitor of the action of prostaglandins in sheep and human semen.


Growth of Theileria parva-infected bovine lymphoid cells in whole-body irradiated mice.


Treatment of infective endocarditis--1973.


Proceedings: Radiation treatment of carcinoma of the nasal vestibule.


Proceedings: Electron beam therapy of head and neck tumors.


Proceedings: Time dose factors in radiation induced osteitis.


Proceedings: Experimental verification of a method for varying the effective angle of wedge filters.


Serologic studies in farmer's lung. Precipitins to the thermophilic actinomycetes.


Primary meningococcal pneumonia.

Primary meningococcal pneumonia.


Possibilities and limitations of delegated literature searches by computer.


A variation of the sulfide contraction procedure for synthesis of corrinoid systems.


Formation of a 1-hydroxycorrin complex by photochemical A-D-secocorrin leads to corrin cycloisomerization.


Photographing specimens immersed in fluid.


Brain permeability of water.

Brain permeability of water.


A monoclonal IgM with antibodylike specificity for phospholipids in a patient with lymphoma.


Plasma cell dyscrasia associated with the production of incomplete (?deleted) IgGlambda molecules, gamma heavy chains, and free lambda chains containing carbohydrate: description of the first case.


Cerebrovascular Moyamoya disease.

Cerebrovascular Moyamoya disease.


Insulin and growth hormone secretion in rats with ventromedial hypothalamic lesions maintained on restricted food intake.


Insulin degradation. IX. On the presence of glutathione-insulin transhydrogenase in human leukocytes.


Sodium retention accompanying insulin treatment of diabetes mellitus.


Kinetic studies of levansucrase of Bacillus subtilis.


Phasing effects of light on cell division in exponentially increasing cultures of Tetrahymena grown at low temperatures.