Effects of antithymocyte sera and antimacrophage sera on cell-mediated immune reactions in Listeria-infected mice. The cell-mediated immune responses of allograft rejection, delayed hypersensitivity, and resistance to Listeria monocytogenes were suppressed by injections of antithymocyte serum (ATS), but the immune responses were not significantly altered by antimacrophage serum (AMS) or normal rabbit serum (NRS). Antisera were prepared in rabbits against purified mouse thymocytes and purified peritoneal macrophages. When mice were injected with ATS near the time of skin grafting, allografts survived significantly longer. Similar administration of AMS or NRS failed to alter the course of graft rejection. Decreased footpad swelling indicated the suppression of delayed hypersensitivity in mice injected with ATS 6 days after a sublethal inoculation of Listeria cells. None of the serum treatments affected the ultimate survival of mice infected with a small number of bacteria. Either ATS or NRS was injected into immunized mice 1 day before and 2 days after a challenge inoculation of Listeria cells. Pronounced suppression of delayed hypersensitivity was found in the ATS-treated groups, along with extensive mortalities that reached 100% in the group receiving the largest dose of ATS. All control animals survived and demonstrated strong delayed hypersensitivity reactions. Antimacrophage serum had no significant effect on the three mechanisms of cell-mediated immunity that were tested. The lymphoid cells which mediate delayed hypersensitivity, antimicrobial cellular immunity, and allograft rejection possess antigenic determinants in common with thymocytes.


Lipids of putative relevance to virulence in Mycobacterium tuberculosis: correlation of virulence with elaboration of sulfatides and strongly acidic lipids. From examination of some 40 patient strains of Mycobacterium tuberculosis, a statistically very significant correlation (Spearman's rho) can be drawn between the root index of virulence for the guinea pig (D. A. Mitchison) and the ability of the individual strains to elaborate strongly acidic lipids (SAL) in culture. These include both sulfatides (SL) and phospholipids (PL). Since essentially all, if indeed not all, of the virulent and only few attenuated strains are prolific in elaborating SAL, this criterion may be a necessary requirement for the expression of virulence in M. tuberculosis. Tested by chi-square, this premise is seen to be statistically and pragmatically highly significant. We speculate that SL may contribute to the pathogenesis of tuberculosis because of a demonstrable activity directed against host liver mitochondrial membranes (manuscript in preparation) and its synergistic potentiation of the specific toxicity of trehalose dimycolate (cord factor). The activity may also be expressed against phagosomal and lysosomal membranes within macrophages. Because of their strongly anionic character, SL and PL may interact with cationic sites on lysosomal hydrolases with resultant immobilization and/or inactivation of the enzymes. By a similar mechanism, these ionic lipids may alter the activity of bactericidal basic proteins, previously recognized in the lysosomal armamentarium. Since a minor but significant fraction of demonstrably attenuated strains is nevertheless prolific in SL or SAL elaboration, this facility alone is evidently not a sufficient criterion for expression of virulence.


Characterization and serotyping of three feline reovirus isolates. Three feline virus isolates were shown to be members of the reovirus group by their growth characteristics in cell cultures, physicochemical properties, and appearance under an electron microscope. Their close serological relationship to, or identity with, human reovirus type III was revealed by hemagglutination, hemagglutination inhibition, serum neutralization, and gel diffusion tests. One feline isolate (636) was pathogenic for suckling mice.


Release of beta-lysin from platelets by thrombin and by a factor produced in heparinized blood. Removal of calcium by either citrate, oxalate, or ethylenediaminetetraacetate inhibited coagulation of blood at an early stage and prevented the release of beta-lysin from platelets. In contrast, heparin caused platelet agglutination and stimulated beta-lysin release in vitro and in vivo. This release was calcium dependent and may have been due to a calcium-dependent reaction in the blood coagulation sequence. Thrombin which by-passed the early calcium-dependent stages of coagulation directly stimulated the release of beta-lysin from platelets. However, thrombin alone or in combination with other plasma factors was not as effective in releasing beta-lysin as the regular coagulation process. Thrombin's platelet degranulating activity correlated with its beta-lysin releasing activity. In contrast to thrombin, staphylococcal coagulase, which also by-passed the calcium-dependent stages of coagulation, coagulated citrated blood without releasing beta-lysin. The release of beta-lysin has been observed previously in the absence of blood coagulation, but this is the first observation of coagulation without beta-lysin release. It is clear that beta-lysin is released from platelets during coagulation by the direct action of thrombin and that it may be released in an earlier calcium-dependent reaction.


Action of group A streptococcus extracellular product(s) on the connective tissue of the bovine heart valve. Group A streptococcal strains isolated from rheumatic fever patients were cultivated in the presence of bovine heart valves in a medium devoid of components from animal origin. Other group A streptococci and various bacteria were used as controls. The supernatant of these cultures was extracted and analyzed chemically and immunologically. The extracts prepared from cultures of two "rheumatogenic" strains in the presence of bovine heart valves showed fraction(s) containing proteins, oses (neutral and amined), and uronic and sialic acids. This fraction(s) was immunologically active with both anti-group A streptococcus and antisoluble connective glycoprotein antisera, with a partial identity reaction. Experiments with a diffusion chamber and attempts to precipitate a postulated enzyme from the culture of these strains by ammonium sulfate suggest that this action is due to an extracellular product of the bacteria. The meaning of these data in the physiopathology of rheumatic cardiac lesions is briefly discussed.


Role of mycobactin in the growth and virulence of tubercle bacilli. Tubercle bacilli failed to grow in iron-void media enriched with solutions of iron-containing transferrin (Tr) or ferritin (F) because these substances do not provide the bacilli with iron, which is essential for their growth. Animal serum and macrophages possessed no iron carrier with an ability to satisfy the need of the bacteria for the metal. Mycobactin (M), the growth-product of tubercle bacilli, removed iron from Tr and F and supplied the metal for bacillary utilization. The role of M in the growth of tubercle bacilli was influenced by nonionic surfactants which inhibited bacillary growth by removing M from the bacillary cells and interfering with the absorption of M-iron complexes. Experiments with Tween 80, Triton WR-1339, and lecithin showed that avirulent bacilli lose M at lower concentrations of the surfactants than virulent bacilli. Since avirulent and virulent bacilli possess the same amount of M, these findings indicate that M is bound more firmly to lipid-rich virulent than lipid-poor avirulent cells. These findings indicate that the resistance of virulent bacilli to the M-removing activity of the surfactants is an indicator of their ability to multiply in the infected host and may be used as a measure of bacillary virulence.


Reactivity of purified proteins and polysaccharides from Mycobacterium tuberculosis in delayed skin test and cultured lymphocyte mitogenesis assays. Hypersensitivity responses to two highly purified and well characterized mycobacterial proteins and a preparation of mycobacterial arabinogalactanarabinomannan were studied by using guinea pig skin tests and in vitro-cultured human lymphocyte mitogenesis stimulation assays. The purified proteins were found to elicit delayed-type hypersensitivity skin test reactions and to stimulate mitogenesis in cultured lymphocytes. The polysaccharide preparation was found to evoke delayed skin tests but not to stimulate cultured lymphocytes.


Benign intra-osseous cysts of the jaws.

Benign intra-osseous cysts of the jaws.


Antigens of Streptococcus mutans. II. Characterization of an antigen resembling a glycerol teichoic acid in walls of strain BHT. Cold 10% trichloroacetic acid was used to extract antigens from purified cell walls of Streptococcus mutans BHT. Column chromatography on Biogel P-100 resolved two serologically reactive fractions (B and C). These fractions were ascertained to be relatively pure by recycling on Biogel P-100, Ouchterlony double-diffusion analysis, and immunoelectrophoresis. Fractions B and C demonstrated bands of absolute homology by double-diffusion but different mobilities by immunoelectrophoresis. Chemical analysis indicated that fraction B is a polysaccharide composed principally of rhamnose and galactose, with smaller amounts of glucose and glucosamine. Small quantities of glycerol and phosphorus also were found. Fraction C was composed mainly of galactose, glycerol, and phosphorus. Alkaline hydrolysis of this fraction yielded products typically released by the degradation of a glycerol teichoic acid, such as glycerol monophosphate, glycerol diphosphate, inorganic phosphorus, and several glycosyl glycerol phosphates. Diglycerol triphosphate was not detected. Side-group analysis revealed that glycerol was substituted by mono- and trigalactosyl moieties. Fraction C was deduced to contain 25 glycerol phosphate units per polymer length. Hapten inhibition studies revealed a beta-galactoside as the probable hapten on this antigen. The BHT teichoic acid reacted strongly with FA-1 antiserum. It showed bands of homology with both BHT and FA-1 crude acid extracts upon double-diffusion, using antisera to either strain. The BHT teichoic acid also displayed immunoelectrophoretic behavior identical to one of the mobile FA-1 cell wall antigens, again using either serum to develop precipitin bands. It is concluded this antigen may possess a serotype-specific determinant for S. mutans serotype b.


A survey of school dental health programs in the state of Connecticut.


A national leader observes and reflects.


An immunodiffusion analysis of Mycobacterium lepraemurium Marchoux and Sorel.


The sensitivity of Brucella abortus to chemotherapeutic agents.


The serological response to Aspergillus fumigatus in experimental mycotic abortion in sheep.


Pyocin-sensitivity testing as a means of typing Pseudomonas aeruginosa.


Proceedings: The effect of tissue-culture cells in promoting prolonged survival of Neisseria gonorrhoeae in artificial subcutaneous cavities of mice.


Proceedings: Corynecine typing of diphtheria bacilli.


Letter: Crystallization and preliminary x-ray investigation of bovine erythrocyte carbonic anhydrase.


Electrophoretic and immunological studies on the light chains of myosin.


Relation between seramarcide, an antitussive for dogs and serratigen from Serratia marcescens.


Neuroleptanalgesia in small animals.

Neuroleptanalgesia in small animals.


Circulatory effects of splenectomy in the horse. I. Effect on red-cell distribution and variability of haematocrit in the peripheral blood.


Circulatory effects of splenectomy in the horse. II. Effect on plasma volume and total and circulating red-cell volume.


One course Sue Barton never took: health care economics.


Experiments on the hepato-oesophageal artery of the mouse.


Intra-abdominal pressure in small ruminants.


Immunochemical characteristics and differentiation of Nocardiae.


Effect of contractions in vitro on glycogen content and glycogen synthetase activity in muscle.


Differences in the autoradiographic localization of labelled morphine-like analgesics in the mouse.


Effects of insulin and testosterone on glycogen synthesis and glycogen synthetase activity in rat levator ani muscle.


The effect of insulin on epinephrine and glucagon inactivated glycogen synthetase I in the isolated perfused rat liver.


Influence of formaldehyde-treated casein supplements on the concentration of epsilon-N-methyllysine in sheep plasma.


Air-blast avoidance learning sets in protein-malnourished monkeys.


The role in chylomicron formation of phospholipase activity of intestinal Golgi membranes.


The visual fields of the frog and toad: a comparative study.


Nitrogen fixation by Anabaena cylindrica. II. Nitrogenase activity during induction and aging of batch cultures.


Studies on goat anti-CBA thymocyte globulin: the large scale preparation, purification and testing of anti-lymphocyte serum and anti-lymphocyte globulin.


The occurrence of two types of synthesis of deoxyribonucleic acid during normal growth in Bacillus subtilis. A study of the relative utilization of thymine and thymidine as precursors for DNA synthesis during normal growth in Bacillus subtilis showed that thymine serves preferentially as a precursor for ;repair' synthesis, whereas thymidine is used preferentially for ;replicative' synthesis. Further, evidence was obtained which suggests that during normal growth both ;replicative' and ;repair' DNA syntheses occur simultaneously. ;Repair' synthesis is distinguished not only on the basis of its preferential utilization of thymine but also by its selective inhibition by caffeine. ;Replicative' synthesis, however, is selectively inhibited by 6-(p-hydroxyphenylazo)-uracil. ;Repair' synthesis would seem to be a ;pre-fork' phenomenon and its inhibition is highly lethal to the cell.


A teaching course in general practice.

A teaching course in general practice.


The structure of a polymer containing galactosamine from walls of Bacillus subtilis 168. Cell walls of Bacillus subtilis 168 contain a phosphorylated polysaccharide composed of N-acetylgalactosamine, glucose and phosphate. Controlled acid hydrolysis gives 3-O-beta-d-glucopyranosyl-N-acetylgalactosamine with a phosphomonoester group at the 6-position of glucose. It is likely that the polysaccharide consists of disaccharide units connected by phosphodiester residues joining the 1-position of N-acetylgalactosamine to the 6-position of glucose in the neighbouring unit.


Interocular transfer in split-brain monkeys following serial disconnection.


Cold fiber heat sensitivity: dependency of "paradoxical" discharge on body temperature.


Selective removal of albumin from plasma by affinity chromatography.


Cell-mediated immune response in malnutrition.


Immune responses in congenitally thymus-less mice. II. Quantitative studies of serum immunoglobulins, the antibody response to sheep erythrocytes, and the effect of thymus allografting.


Universal occurrence of antibodies to tubercle bacilli in sera from non-tuberculous and tuberculous individuals.


Rosetting of human red blood cells to thymocytes and thymus-derived cells.


Drosophila salivary chromosomes as test system for antinuclear antibody assay.


Antigenic changes in transformed FL human amnion cells.


Sensitivity of non-human cells to human lymphocyte 'proliferation inhibitory factor' (PIF) in vitro.


Inhibition of IgG complex-induced platelet aggregation by antiglobulin-active cryoglobulin IgM components.


Immunochemical studies of four human IgM pyroglobulins.


Further studies on the C3b inactivator or conglutinogen activating factor (KAF).


Comparison of the capillary tube and explant methods for the in vitro assay of tuberculin. Purified antigens prepared from mycobacteria were tested for nonspecific toxicity and tuberculin activity by two in vitro methods. One technique utilized measurements of macrophage migration in 4-day-old cultures of spleen from normal and tuberculin-sensitive rabbits. The other method was a modification of the capillary tube technique. To obtain enough peritoneal macrophages for good quantitation, changes were made in the method of harvesting cells, and in some instances exudates from two or more Wright strain no. 13 guinea pigs were combined. The capillary tube method was as sensitive as the explant method for detecting nonspecific toxicity. Each tuberculin assay included a group of control cultures of cells from sensitive animals, test groups containing two widely spaced concentrations of a standard tuberculin, PPD-S, and one or more concentration of the tuberculin to be tested. Macrophages from tuberculin-sensitive animals were regularly inhibited by 0.25 mug of PPD-S per ml with both in vitro methods. The potency of the test tuberculins relative to that of PPD-S was somewhat greater in capillary tube assays than in explant cultures. A purified tuberculopolysaccharide was equally inhibitory for both normal and tuberculin-sensitive cells in both culture systems.


Comparison of strains of BCG. I. Antigenic analysis and tuberculin reactivity. At least 10 of 11 demonstrable antigens in unheated culture filtrates of 12 vaccine strains of BCG were shared as determined in a reference antigen-antibody immunoelectrophoresis system. Filtrates from each of the BCG strains gave equivalent skin test reactions in homologously and heterologously sensitized guinea pigs. By these immunological parameters, the 12 BCG strains were remarkably similar.


Quantitative chemical composition of peptidoglycan of Listeria monocytogenes. The qualitative and quantitative chemical composition of the peptidoglycan layer of Listeria monocytogenes cell walls was determined. The peptidoglycan layer was isolated from intact cells after treatment with sodium lauryl sulfate and digestion with Pronase. The isolated peptidoglycan contained carbohydrates which included hexose, rhamnose, and amino sugars. The amino sugar was identified as glucosamine which was present in very high micromole concentration, as compared with the amino acids, muramic acid, or diaminopimelic acid. In addition, protein and phosphorus were also present. However, the presence of teichoic acid was not detected in the peptidoglycan. Protein moiety contained a total of 18 amino acids with lysine as the N-terminal amino acid.


Extracellular antigens from Listeria monocytogenes. II. Cytotoxicity of hemolytic and lipolytic antigens of Listeria for cultured mouse macrophages. Purified preparations of hemolytic and lipolytic antigens were shown to be cytotoxic for tissue cultures of mouse peritoneal macrophage monolayers. The percent reduction in viability was proportional to the concentration of antigen used although, for a brief period, the hemolytic antigen stimulated the growth of cultured macrophages. Hemolytic and lipolytic antigens were synthesized by an hemolysin-producing strain (7973), but were deficient or totally lacking in nonhemolysin-producing strains (9037-7, 4219). The inoculation of strain 7973 onto macrophage monolayers at a bacterium to macrophage ratio of 10:1 resulted in a complete loss of viable phagocytic cells. The decrease in viable phagocytic cells was accompanied by an increase in viable listeria. Pronounced cytopathic activity with proliferation of listeria was also observed when 200 U of lipolytic antigen were added to macrophage monolayers that had been infected with the smooth type nonhemolysin-producing strain (4219) on the previous day. On the other hand, a high percentage of macrophages survived when exposed to nonhemolysin-producing strains only. In this case, listeria were not recovered by routine culture methods. The data presented do not establish that phagocytic cell destruction with accompanying microbial proliferation is the direct result of hemolysin elaboration; however, they suggest that there is need for further exploration of the role of the hemolysin in listeriosis.


Enzyme-linked immunosorbent assays for cholera serology. The enzyme-linked immunosorbent assay (ELISA) principle of Engvall and Perlmann, which employs antigen-coated tubes and enzyme-labeled anti-immunoglobulins, was elaborated for use in cholera serology. Immunoglobulin class-specific determinations of primary binding titers of antibodies to cholera exotoxin and endotoxin were more sensitive than were neutralization and vibriocidal tests. It was notable that rather high ELISA titers of immunoglobulin M (IgM) antibodies to exotoxin, which lacked neutralizing capacity were registered, whereas corresponding levels of immunoglobulin G (IgG) antibodies were effectively neutralizing. A modified technique permitting measurement of the binding rate of antibody to the solid-phase antigen was introduced as a possible tool for antibody avidity estimation. Such measurements indicated a wide avidity difference between the IgG and the IgM anti-exotoxin antibodies, which could explain their different neutralizing capacities. The observation that increasing binding rate of the IgG antibodies during the course of the primary immune response could compensate for decreasing antibody amount with regard to neutralizing capacity of serum also indicated the importance of antibody avidity for toxin neutralization. Inhibition with soluble antigen permitted quantitative determination of antigen; levels of exotoxin 0.09 mug/ml and of endotoxin to 1.3 mug/ml were measured.


Thermography of tuberculin reactions in cattle. The allergic reactions of dermal induration and hyperthermia were compared in tuberculin-hypersensitive calves. Tuberculins prepared from Mycobacterium avium, M. bovis, and M. paratuberculosis were administered intradermally and the responses were compared. The indurations from immediate and delayed hypersensitivity reactions were readily distinguishable, but the hyperthermic responses appeared to contain elements of both immediate and delayed hypersensitivity.


Diffusion artifacts in cytochemistry of catalase.


Experimental induction of anergy to coccidioidin by antigens of Coccidioides immitis. Failure to react to coccidioidin (anergy) often occurs in patients with disseminated coccidioidomycosis. One possible reason may be desensitization by excessive amounts of antigen. This was studied experimentally by injection of soluble and hyphal antigens of Coccidioides immitis into coccidioidin- and tuberculin-sensitive guinea pigs. Guinea pigs sensitized by injection of killed hyphal cells of C. immitis in complete Freund adjuvant were subsequently injected daily either with soluble coccidioidal antigen administered intraperitoneally or with hyphal antigen administered either subcutaneously or intraperitoneally. Gradual loss of cutaneous reactivity to coccidioidin occurred, but the reactivity to tuberculin remained unimpaired. The rapidity of desensitization was roughly proportional to the dose of antigen with desensitization occurring as early as 6 days after beginning injections. This anergic state was temporary, and reactivity returned several days after discontinuing injection of antigen. Injection of coccidioidal antigen led to production of coccidioidal complement-fixing antibody, but there was no consistent relationship between the antibody titer and state of cutaneous reactivity to coccidioidin. Peritoneal exudate or pulmonary alveolar cells from desensitized animals migrated freely in the presence of coccidioidin but were inhibited in the presence of tuberculin. Heat treatment did not impair the capacity of the soluble or hyphal antigen to induce anergy, thus suggesting that the antigen active in complement fixation was perhaps not involved in desensitization. Polysaccharide obtained by ethanol precipitation of dialyzed coccidioidin failed to induce anergy. Dialysis of the soluble coccidioidal antigen caused the loss of the desensitizing activity. Thus, specific desensitization could be induced by administration of large doses of coccidioidal antigen but dialyzable components appear important in this desensitization.


A steady state model of sequential irreversible enzyme reactions.


Possible transmission of serum (Australia-antigen-positive) hepatitis via the conjunctiva. A nursing sister developed Australia-antigen-positive hepatitis 101 days after the blood of an Australia-antigen-positive patient was accidentally introduced into her eye. There may be important implications for those caring for patients with Australia-antigenemia or undertaking laboratory investigations on their blood.


Cytological localization of Dengue-2 antigens: an immunological study with ultrastructural correlation. Immunofluorescence and electron microscopy were used to study the development and cytological distribution of dengue-2 virion and nonvirion antigens in monkey kidney cells. The type and combination of fixatives were found to affect the intensity of fluorescence. Paraformaldehyde fixation alone resulted in a low level of fluorescence, but an additional fixation step with ethanol or acetone resulted in maximum staining. No fluorescence was obtained after fixation with glutaraldehyde alone or in combination with other solvents. Methanol caused selective ablation of fluorescence by antibody to purified virions. Dengue-specific fluorescence that was most intense in the perinuclear area radiated in a granular pattern of decreasing intensity into the cytoplasm. The perinuclear fluorescence was associated with nonvirion antigens and the cytoplasmic fluorescence was associated with virion antigens. Electron micrographs of infected cells revealed vesicular bodies with reticular electron-dense centers, clusters of structurally complete virions deep within the endoplasmic reticulum, and single virus particles in vacuoles near the periphery of the cell.


Malnutrition and hepatitis-associated antigen infection.


Infantile malnutrition and malabsorption.


Visual discordance cues induce prism adaptation in normal monkeys.


White blood cell acid hydrolases in leukaemias, mucopolysaccharidoses and mannosidosis.


Maternal protein deprivation and toxemia of pregnancy: studies in the rhesus monkey (Macaca mulatta).


Prolactin secretion in sixty-five patients with galactorrhea.


Thiol solutions--inhibition of autoxidation.


Simplified methods for automated ion-exchange separation of peptides and accelerated manual Edman degradations.


Juvenile xanthogranuloma in a patient with Niemann-Pick disease.


A spectrophotometric assay for Drosophila dopa decarboxylase.


Comparative effects of dihydroergotamine and hydergin on the blood flow, capillary filtration rate and the capacitance vessels in the human calf studied by plethysmography.


The relationship between progestins and gonadotropins during the late luteal phase of the menstrual cycle in rhesus monkeys.


Thyrotropin-releasing hormone in the hypothalamus of the rat.


Sexual maturation in the female rhesus monkey and the development of estrogen-induced gonadotropic hormone release.


Immunological techniques.

Immunological techniques.


Osmotic stimulation of vasotocin secretion by the toad's hypothalamo-neurohypophyseal system in vitro.


Effect of L-asparagine on growth of Mycobacterium tuberculosis and on utilization of other amino acids. l-Asparagine controls the utilization of other amino acids by Mycobacterium tuberculosis (H37Ra) in aerated, liquid synthetic media. In a mixture containing asparagine and either l-alanine or l-glutamic acid, amino acid utilization is diphasic, with asparagine being utilized first. Short-term growth rates and cell yields are diminished and mimic those seen with asparagine alone. Catabolite repression is the probable regulatory mechanism responsible for this effect of asparagine. In contrast, in the presence of aspartic acid, asparagine stimulates growth and increases utilization of aspartic acid.


Absence of 3'-terminal residues from transfer ribonucleic acid of dormant spores of Bacillus megaterium. Essentially all (>97%) of the transfer ribonucleic acid (tRNA) in log-phase and sporulating cells of Bacillus megaterium contains a complete 3'-cytidyl-cytidyl-adenosine terminus. However, about one-third of the tRNA in the dormant spore lacks the 3'-terminal adenosine 5'-monophosphate (AMP) residue, and some of the adjacent cytosine monophosphate residues are also missing. Examination of specific tRNAs indicated that those specific for isoleucine, leucine, and methionine are missing 30 to 40% of their terminal residue, whereas tRNAs specific for tyrosine lack 88% of the 3'-terminal AMP. Defective spore tRNA is not degraded during germination, but the missing residues are added back in the first minutes of the process. The enzyme catalyzing the addition reaction, tRNA nucleotidyltransferase, is present in the dormant spore at a level similar to that found in the vegetative cell.


Effect of helium gas at elevated pressure on iron transport and growth of Escherichia coli. Helium at an ambient pressure of 68 at m with 0.2 atm of O(2) shortened by 1 to 1.5 h the lag phase for growth of Escherichia coli in minimal medium supplemented with 2 muliters of cell-free culture filtrate (CFF) per ml or with 1 muM 2,3-dihydroxybenzoylserine (DHBS), an iron chelator. The lag phase of cultures not exposed to helium could be shortened by use of supplements, but higher concentrations were required-10 to 30 muliters of CFF per ml or 10 to 50 muM DHBS. Strain AN 193 of E. coli, which requires the DHBS precursor 2,3-dihydroxybenzoic acid (DHBA), grew well in media with 10 muM DHBA when exposed to helium at 68 atm, whereas 100 muM DHBA was required for growth in unexposed cultures. In the presence of 100 muM DHBA plus 1.0 muM ethylenediaminetetraactic acid, growth was inhibited at 1 and 68 atm. Growth was restored, however, by the addition of 0.1 muM FeSO(4) at 68 atm and 1.0 muM FeSO(4) at 1 atm, but lag times were invariably shorter in the pressurized cultures. Hydrostatic pressures of 68 atm did not reduce the lag phase in the presence of CFF, DHBS, or DHBA. Our results suggest that 68 atm of helium pressure, but not hydrostatic pressure, elicited a more rapid transport of iron into the cells.


Characterization of three plasmid deoxyribonucleic acid molecules in a strain of Streptococcus faecalis: identification of a plasmid determining erythromycin resistance. Three plasmids designated alpha, beta, and gamma, distinguishable by their molecular weights (6, 17, and 34 million, respectively) were isolated from Streptococcus faecalis strain DS-5 (ATCC 14508). Derivatives of this strain "cured" for erythromycin resistance lacked the beta-plasmid. In the parent strain the beta-plasmid was estimated to be present to the extent of one to two copies per chromosomal genome equivalent whereas the alpha- and gamma-plasmids were about nine and five copies, respectively.


Phenotype recognition of pyocyanine mutants in pseudomonas aeruginosa. Certain classes of pyocyanine mutants in Pseudomonas aeruginosa escape detection when screened in the presence of wild-type or other mutant cells. A technique is described for recognizing mutant phenotypes after cells are in individual agar wells. The procedure eliminates cross-feeding phenomena as well as the masking of mutant phenotypes by diffusing pyocyanine produced by nearby clones.


Ultrastructural and chemical alteration of the cell envelope of Pseudomonas aeruginosa, associated with resistance to ethylenediaminetetraacetate resulting from growth in a Mg2+-deficient medium. Cells of Pseudomonas aeruginosa became resistant to the lytic effect of ethylenediametetraacetate (EDTA) when grown in a Mg(2+)-deficient medium. To correlate ultrastructural changes in the cell wall associated with the shift to EDTA-resistance, a freeze-etch study was performed. Upon fracturing, the outer cell wall membrane split down the hydrophobic center to reveal the outer (concave) and inner (convex) layers. The concave cell wall layer of EDTA-sensitive cells grown in Mg(2+)-sufficient medium contained spherical units resting on an underlying smooth support layer. Upon EDTA treatment, approximately one-half of these spherical units were extracted. Cells grown in Mg(2+)-deficient medium were resistant to EDTA. The concave cell wall layer of EDTA-resistant cells had increased numbers of highly compacted spherical units, giving this layer a disorganized appearance. The highly compacted appearance of this layer was unaltered by EDTA treatment. Thus, growth in Mg(2+)-deficient medium resulted in cells which were resistant to EDTA and which possessed an ultrastructurally altered outer layer of the outer cell wall membrane. Cell envelopes from EDTA-resistant cells were found to possess 18% less phosphorus, 16.4% more total carbohydrate, and 13.3% more 2-keto-3-deoxyoctonate than cell envelopes from EDTA-sensitive cells. There were also qualitative, but not quantitative, differences in the protein content of cell envelopes from EDTA-resistant and EDTA-sensitive cells.


Mapping of the 5-methyltryptophan resistance locus in Bacillus subtilis. The 5-methyltryptophan resistance locus (mtr) in Bacillus subtilis, which leads to constitutive production of the tryptophan enzymes, has been mapped on the chromosome. The order of loci is ser-1-mtr-aroF-aroB-trp-hisB.


Changes in menaquinone concentration during growth and early sporulation in Bacillus subtilis. In two strains of Bacillus subtilis, menaquinone-7 has been shown to reach maximal cellular concentrations during early stationary phase. These concentration changes closely parallel the previously reported concentration changes in the cytochromes.


Pneumothorax complicating continuous ventilatory support.


Characterization of the antigenic subunits of the envelope protein of Yersinia pestis. Chemical, physical, and immunological properties of the envelope antigen of Yersinia pestis strains have been investigated. The antigen consists of two components with isoelectric points (pI) of 4.6 and 4.8. One component (pI 4.6) is a protein bound to a small carbohydrate moiety identified as an oligomeric galactan; the other component (pI 4.8) is a simple protein. These two components are antigenically identical. In buffered solution, the antigen exists as aggregates of molecular weights larger than 300,000. The aggregates dissociate into a variety of smaller molecular weight forms depending on the nature of the treatment for dissociation. Each aggregate can be further dissociated into a single antigenic subunit fraction containing protein and glycoprotein species with molecular weights in the range from 15,000 to 17,000. The subunits can be obtained by a dissociation treatment with 0.1% mercaptoethanol in 0.25% sodium dodecyl sulfate at 95 C for 5 min. The subunits will readily reaggregate into a variety of larger molecular weight forms on the removal of dodecyl sulfate.


Some quantitative aspects of in vitro thyroid diagnostic tests.


Occult blood detection in faeces of various animal species.


Impaired lymphocyte function in untreated Hodgkin's disease.


Letter: Statistical analysis of treatment with vitamin E.


Letter: Urinary sphingomyelin in Niemann-Pick disease.


Letter: Chagas's disease--another hazard in acute leukemia.


Treatment of disseminated gonococcal infection. A prospective evaluation of short-term antibiotic therapy.


Survival of common bacteria in liquid culture under carbon dioxide at high temperatures.


Understanding cerebrovascular function. Contributions of space techniques.


Microbial flora found in the products of conception in spontaneous abortions.


Blood disappearance rates of certain hormones and enzymes advocated as fetoplacental function tests.


Congenital tuberculosis.

Congenital tuberculosis.


Proceedings: Medullary carcinoma of the thyroid gland.


Proceedings: The cost of cancer.

Proceedings: The cost of cancer.


Proceedings: Megavoltage irradiation for bladder cancer: alone, postoperative, or preoperative?


Proceedings: The role of radiation therapy in the management of patients with carcinoma of the rectum and rectosigmoid.


Proceedings: The role of radiation therapy in the treatment of soft-tissue sarcoma in adults.


Neurons in rhesus monkey visual cortex: systematic relation between time of origin and eventual disposition. Autoradiographic evidence after injection of tritiated thymidine indicates that cell position in the laminae of the monkey visual cortex is systematically related to time of cell orgin. The earliest-formed neurons, destined for the deepest stratum, arise at about embryonic day 45, and the last ones, destined for the outermost cell stratum, form at about day 102; cells of intervening layers are generated at intervening times. No neocortical neurons are produced in the last two prenatal months or after birth. Compared to cortical neurons in rodents, those in the monkey arise earlier, and the "inside-out" relation of cell position to time of origin is more rigid.


Deuterium oxide effect on temperature-dependent survival in populations of Drosophila melanogaster. A comparison of the mean life-spans for populations of Drosophila melanogaster at 10 degrees , 20 degrees , 25 degrees , and 30 degrees C maintained on media prepared with distilled water and with 50 percent deuterium oxide shows that deuteration decreases longevity at all four temperatures. The magnitude of the difference between the mean survival times of populations maintained on deuterated and nondeuterated media is inversely related to temperature between 10 degrees and 30 degrees C.


Carbonic anhydrase and osteoclasts: localization by labeled inhibitor autoradiography. Autoradiography with tritiated acetazolamide indicates that osteoclasts of the hen and chick contain concentrations of carbonic anhydrase which are similar to those in pancreatic acinar cells. Grain counts of osteoblasts and osteocytes were not different from background. Thus, a sufficient quantity of carbonic anhydrase seems to be present in osteoclasts to be of physiological importance in bone resorption.


Calcium ion release in mechanically disrupted heart cells. In cardiac muscle fibers which have had their sarcolemma disrupted intracellular stores of calcium ions can be released by the same chemical stimuli which cause their release from the sarcoplasmic reticulum of skinned skeletal muscle fibers. These stimuli are increases in calcium or caffeine concentrations and substitution of chloride for propionate or sodium for potassium in solutions bathing the fibers.


Management of the nephrotic syndrome.

Management of the nephrotic syndrome.


Salivatory fibres in the trigeminal part of the lingual nerve to the mandibular salivary gland of the monkey.


A new approach to the synthesis of oligodeoxyribonucleotides.


Evaluation of heterologous vascular grafts as small vessel substitutes.


Physical measurements of medical electron beams--a review.


Fibroblast surface antigen: a new serum protein.


Studies on the control of some simple motor tasks. 3. Comparison of the EMG pattern during ballistically initiated movements in man and squirrel monkey.


Neuronal migration, with special reference to developing human brain: a review.


Acute stroke management and patient outcome: the value of Neurovascular Care Units (NCU).


Studies on the control of some simple motor tasks. IV. Relation between parameters of motion and motor output in squirrel monkey.


Responses of small myelinated "warm" fibers to noxious heat stimuli applied to the monkey's face.


Visual discrimination deficits associated with posterior inferotemporal lesions in the monkey.


Operable breast cancer: the case against conservative surgery.


Operable breast cancer: in defense of conservative surgery.


Colon and rectal lesions: a tutorendoscopic view.


Features suggesting curability in lymphoma and leukemia.


Phenotypic variation in the popliteal pterygium syndrome.


Mass screening newborns for mucopolysaccharidoses.


Assays for cellular antigens in the presence of detergents.


Homogeneity of antibodies produced by clones in vitro.


Antibody response, measured by a plaque assay, compared to cellular response after skin allograft in mice.


Carbon dioxide production from pyruvate and glucose by bovine oocytes.


Protein incorporation by isolated amphibian oocytes. IV. The role of follicle cells and calcium during protein uptake.


Lysosomal enzymes in relation to the isoproterenol-induced secretory cycle in rat parotid gland.


Ontogeny of gamma-crystallin in the chick lens.


Proceedings: Anatomy and pathophysiology of ocular circulation.


Proceedings: Studies on retinal blood flow regulation: the effect of PaCO2 on blood flow, oxygen availability, oxygen consumption rate and ERG in monkeys.


Proceedings: A comparison of some derivatives of phloretin as prostaglandin antagonists.


Proceedings: The effect of increased intraocular pressure on the endothelium of Schlemm's canal.


Proceedings: Experimental iris suture in primates.


Unequivocal identification of sphingomyelin in the lipids of Tetrahymena pyriformis.


Behavioral effects of tularemia and sandfly fever in man.


Interaction of lipopolysaccharides with mitochondria. II. Effects of magnesium ions on toxicity of a rough lipopolysaccharide.


Immune response of the human respiratory tract. II. Cell-mediated immunity in the lower respiratory tract to tuberculin and mumps and influenza viruses.


Growth of measles virus in the lymphoid tissues of monkeys.


Immunogenicity of group A and group C meningococcal polysaccharides in human infants.


Inhibition of DNA and protein synthesis in melanocytes by a melanoma extract.


Pancreatic-type tissue in livers of rats fed polychlorinated biphenyls.


Replication of bacteriophage SH-133 in the facultative autotroph Hydrogenomonas facilis. I. Bacteriophage synthesis under heterotrophic, autotrophic, and mixotrophic growth conditions. The ability of bacteriophage SH-133 to replicate in heterotrophically (H-) and autotrophically (A-) grown Hydrogenomonas facilis was examined. Both the synthesis of infectious phage particles and the efficiency of plating (EOP) were reduced by 90% in A-grown cells. Adsorption of phage and lethal effects on H. facilis were identical in both systems. One-step growth experiments showed that cell lysis preceded the appearance of infectious particles in A-grown cells. Burst size studies with mixotrophically grown cells did not indicate the presence of an inhibitor of phage synthesis indigenous to autotrophic metabolism. DNA synthesis was identical in H- and A-grown infected cells; however, protein synthesis was significantly reduced in A-grown infected cells when compared with protein synthesis in H-grown infected cells. The data suggest that the reduction in EOP and phage synthesis in A-grown cells is caused by a defect in viral protein synthesis which results in the limited production of an essential viral protein at the time of cell lysis.


Synthesis of the unusual DNA of Bacillus subtilis bacteriophage SP-15. Cultures of Bacillus subtilis infected with phage SP-15 were examined to investigate the metabolic origin of two of the unique components of the phage DNA: the component responsible for the unusually high buoyant density in CsCl and the unusual pyrimidine, 5-(4', 5'-dihydroxypentyl) uracil (DHPU). Newly synthesized pulse-labeled DNA was light in buoyant density and shifted to the high density of mature phage DNA upon further incubation. Parental DNA was converted to a light-density intermediate form prior to replication. When labeled uracil, thymidine, or DHPU were added to infected cells, it was found that only uracil served as the precursor to DHPU and thymine in phage DNA. Analysis of the bases from hydrolyzed DNA of labeled phage or infected cells indicated that the uracil was incorporated into the DNA as such (presumably via deoxyuridine triphosphate) and later converted to DHPU and thymine at the macromolecular level. The sequence of events after phage infection appeared to be: (i) injection of parental DNA; (ii) conversion of parental DNA to a light form; (iii) DNA replication, yielding light DNA containing uracil; (iv) conversion of uracil to DHPU and thymine; and (v) addition of the heavy component.


Replication of bacteriophage SH-133 in the facultative autotroph Hydrogenomonas facilis. II. Bacteriophage synthesis and hydrogenase induction under step-up and step-down growth conditions. Autotrophically grown infected cells are able to replicate phage SH-133 after being switched to a heterotrophic environment (step-up growth). The effect of step-down growth on phage replication varies with the choice of organic substrate. Phage replication and the induction of cellular hydrogenase occur under step-down growth from acetate but not peptone broth. The requirement of a continued source of energy for phage replication in either heterotrophically or autotrophically grown cells could be uniquely demonstrated in this phage-host system by the deletion of hydrogen from the growth medium.


Viral protein synthesis in bacteriophage phi 29-infected Bacillus subtilis. Twenty-three (14)C-labeled phage phi29-specific proteins in lysates of UV-irradiated Bacillus subtilis have been resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis and identified by autoradiography. Included in this group of proteins are the six major structural proteins of the virion. Analysis of the temporal sequence of viral protein synthesis indicates that three groups of proteins can be identified by time of appearance, beginning at 2 to 4, 4 to 6, or 8 to 10 min after infection, respectively. These proteins account for approximately 90% of the coding capacity of the phi29 genome.


Isolation and characterization of a bacteriophage for Thiobacillus novellus. A DNA-containing bacteriophage for Thiobacillus novellus has been isolated from sewage and purified by ammonium sulfate precipitation, differential centrifugation, and cesium chloride equilibrium centrifugation. The buoyant density of this phage in CsCl is 1.51 g/cm(3). Electron microscopy studies have revealed a polyhedral head about 60 nm in diameter and a tail surrounded by a number of fine filaments. It has an adsorption rate constant of 1.1 x 10(-9) ml/min, a latent period of 45 min, and an average burst size of 150. The mole guanine and cytosine content in its DNA has been estimated to be 57 to 58%. Five structural proteins with molecular weights of 62,000, 42,500, 30,500, 17,750, and 13,500 have been detected by polyacrylamide gel electrophoresis techniques.


Characterization of virus-specific RNAs from subacute sclerosing panencephalitis virus-infected CV-1 cells. CV-1 cells infected with subacute sclerosing panencephalitis (SSPE) virus incorporated uridine-(3)H into at least four virus-specific RNA components in the presence of actinomycin D. The component sedimenting fastest had a sedimentation coefficient of 50s corresponding to a molecular weight of 6 x 10(6). The other three RNA components have sedimentation constants of 35s, 22s, and 18s corresponding to molecular weights of 2.5 x 10(6), 1.0 x 10(6), and 0.75 x 10(6), respectively. The base composition of the 50s RNA is distinct from that of cellular RNA and comparable with base compositions of viral RNAs of other paramyxoviruses. The base composition of the 18s RNA shows approximate complementarity with the 50s RNA. RNA-RNA annealing experiments using unlabeled 50s SSPE RNA with labeled 18s RNA from cells infected with SSPE virus or measles virus show 100% annealing with 18s SSPE RNA but only 60% annealing with 18s measles RNA. These experiments suggest some differences between the 18s RNAs of SSPE virus-infected cells and measles virus-infected cells.


Triiodothyronine. Physiological and clinical significance.


Letter: Jet lag ketoacidosis.

Letter: Jet lag ketoacidosis.


Intracellular components associated with Chikungunya virus-specific ribonucleic acids in infected BHK21 cells.


Studies on murine leprosy bacillus. VII. Temperature range and temperature optimum for the growth of the supposed murine leprosy bacillus.


FP5 factor, an undescribed sex factor of Pseudomonas aeruginosa.


Control of infections from intravenous infusions.


Immunological characterization of anti-haemophilic factor A related antigen in haemophilia A.


Failure to thrive, in Lebanon. II. An investigation of the causes.


Determination of glomerular filtration rate in the newborn. Comparison between results obtained by the single injection technique without collection of urine and the standard clearance technique.


Effect of fasting and of a protein free diet on amino acid ratios in plasma and erythrocytes in rats.


Diagnostic significance of serum orosomucoid level in bacterial infections during neonatal period.


Safety aspects on long-term medication.

Safety aspects on long-term medication.


Generalized mucormycosis in the golden-bellied mangabey (Cercocebus galeritus chrysogaster, Lydekker).


Pathological findings on animals in the Royal Zoological Gardens of the Rotterdam Zoo during the years 1963, 1964 and 1965.


A method for determination of the synthesis rate of stable and unstable ribonucleic acid in Escherichia coli.


Therapeutic implications of copper deficiency in Menkes's steely-hair syndrome.


Kinetics of beta-lactamase inactivation of penicillins. II. Effect of competitive inhibitors.


Cryoglobulinaemia in tropical splenomegaly syndrome.


Determination of IgG antiglobulins in rheumatoid disease using insolubilized human IgG.


Carcino embryonic antigens (CEA) and non-specific cross-reacting antigens in feces.


Regional hemodynamic effects of dopamine in the unanesthetized primate: failure to alter flow-dependent hepatic drug clearance.


Evaluation of plasma thyrotrophin response to thyrotrophin releasing hormone in Graves' disease and in hyperactive thyroid adenoma before and after treatment.


Effects of intense auditory stimulation: hearing losses and inner ear changes in the squirrel monkey. II.


Prevention of periodontal disease--a pathologist's view.


Production of anti-idiotypic and anti-class specific antibody using micro amounts of myeloma serum.


Primate head restrainer using a nonsurgical technique.


N-alkylnorketobemidones with strong agonist and weak antagonist properties.


Synthetic trypanocides. 3. Structure-activity relationships.


Thiourea derivatives of 2-aminooxazoles showing antibacterial and antifungal activity.


Calcium and phosphorus homeostasis in horses.


Haematological relationships between New Guinean mothers and their infants.


Effect of carbohydrate load by intravenous infusion in fasted rats on liver glucose-6-phosphate dehydrogenase and malic enzyme.


Fatal cerebral oedema with insulitis in diabetic ketoacidosis.


Effect of glutaraldehyde fixation on erythrocyte agglutinability.


Relation between bacteriocin production and virulence of Streptococcus faecalis var. liquefaciens.


Effect of chorionic gonadotropin on corpus luteum of the monkey.


The isolation of mutants of Aspergillus flavus and A.parasiticus with altered aflatoxin producing ability.


Pathogenic effects of Cladosporium herbarum and Penicillium decumbens.


Some aspects of intracellular parasitism. In intracellular parasitism the host cell is a true and hospitable host. The parasite does not have to break in the door. It has subtle ways of inducing the host to open the door and welcome it in. One of the exciting fields in the future of parasitology is to find out what these ways are and why they are sometimes so highly specific that the cell that invites one parasite in will not open the door to another closely related species. Once inside, the parasite not only exploits nutrients already available in the cell, and the cell's energy-yielding system, but it further induces the cell to assist actively in its nutrition. Like a bandit who has cajoled his way in, the parasite now forces his host to prepare a banquet for him. Finally it may destroy its host cell, as in most of the associations I have described herein, or it may stimulate its host cell to abnormal increase in size or to have an altered metabolism with the formation of new products. Or it may even contribute some positive benefit to the host cell or to the multicellular organism of which the cell is a part, so that the two kinds of organisms then live together in a state of mutualism or symbiosis (26).


Progress of tuberculous diabetes coming under supervision during the years 1953-65 up to July 1972.


Drug resistant pulmonary tuberculosis treated with ethambutol and rifampicin in North East England.


The differential tuberculin test in a BCG vaccinated population.


Use of videotape feedback as a psychotherapeutic nursing approach with long-term psychiatric patients: A pilot study.


Cumulative effects of pulsed exposures of mycobacterium tuberculosis to isoniazid.


Bladder toxicity due to cyclophosphamide. Experimental induction and treatment.


Co-operative controlled trial of a standard regimen of streptomycin, PAS and isoniazid and three alternative regimens of chemotherapy in Britain. A report from the British Medical Research Council.


A possible D(CW) (e) gene complex of the Rh system.


Rapid development of carcinoma in diverticulum of the bladder. A pitfall in conservative management.


Blood group O(H)-like activity in extracts from some invertebrates. A study with catfish and eel sera and three anti-H lectins.


Small scale preparation and clinical use of factor IX-prothrombin complex.


Automated screening test for high titre tetanus antibody in donor plasma.


The role of rheumatoid factor and related human antiglobulins in the radioimmunoassay (Ausria system) for the detection of hepatitis B antigen.


Free zone electrophoresis of amniotic fluid in normal pregnancies and in pregnancies complicated by haemolytic disease.


Studies on reovirus-antigens. I. Preparation of reovirus-specific immune serum in the rabbit by means of active immunization through the scarified skin.


Kinetics of the attachment of radioisotope-labelled adenovirus hemagglutinins to red blood cells and their detachment by antibody and by nonlabelled hemagglutinins of homologous and heterologous types.


Studies on reovirus-antigens. II. Segregation of reovirus-antigens by chromatography on ecteola-cellulose and by centrifugation in a sucrose density gradient.


Effect of water on the bacterial flora of swimmers' ears.


Trigeminal-facial nerve communications. Their function in facial muscle innervation and reinnervation.


Infantile sexuality in monkeys.

Infantile sexuality in monkeys.


Equations of substrate-limited growth: the case for blackman kinetics.


Detection of myeloma cells in the urine sediment.


Polychlorinated biphenyls in the seastar Acanthaster planci.


Demonstration of 2-oxoglutarate: glyoxylate carboligase activity in Tetrahymena pyriformis.


Inhibition of ribonuclease activity by bentonite.


Prefrontal lobe functions and the neocortical commissures in monkeys.


Mesencephalic control of lateral geniculate nucleus in primates. II. Effective loci.


Mesencephalic control of lateral geniculate nucleus in primates. I. Electrophysiology.


Mesencephalic control of lateral geniculate nucleus in primates. 3. Modifications with state of alertness.


A controlled trial of ambulant out-patient treatment and in-patient rest in bed in the management of tuberculosis of the spine in young Korean patients on standard chemotherapy a study in Masan, Korea. First report of the Medical Research Council Working Party on Tuberculosis of the Spine.


Non-flagellate Pseudomonas aeruginosa in pathological material. Three isolates of non-flagellate Pseudomonas aeruginosa obtained from pathological material of human cases are described. Two were found in urinary tract infections and the third in a respiratory infection. They were shown by typing methods to be distinct strains.


Anterior fusion of the spine for infective lesions in adults.


An automated method for the microbiological assay of serum pyridoxal. A fully automated method for the measurement of serum pyridoxal has been developed. Acid phosphatase was used for dephosphorylation and precipitation of the serum proteins was not required. A chloramphenicol-resistant strain of L. casei was used as the test organism and this removed the need for sterilization. The method gives highly reproducible results, and is suitable for population and institutional studies.


Inactivation of endotoxin by serum: a phylogenetic study.


Notes. Studies of amniotic fluid in women infected with rubella.


Neuronal alterations in developing cortical infarction. An experimental study in monkeys.


Cerebral radiation necrosis simulating a brain tumor. Case report.


Vasodilating effect of crude platycodin in anesthetized dogs.


Adrenergic mechanism in Tetrahymena. II. Effect of adrenaline on cell proliferation.


Comparative studies of hepatic nicotine metabolizing enzyme activities in monkeys and dogs.


Change in catecholamine content of Tetrahymena pyriformis W during growth.


Comparison of two test kit methods for detection of occult blood in animal faeces.


Influence of temperature on Drosophila alcohol dehydrogenase polymorphism.


Homologous interference of lymphocytic choriomeningitis virus.


Comparative analysis of interlaboratory variation in drug sensitivity test for Mycobacterium tuberculosis.


Bacteriophage typing of Mycobacterium ranae (Fortuitum). The correlation of lysis by mycobacteriophage BK4 and inositol utilisation.


Haemolytic activity of various strains of Acinetobacter.


Production of cold agglutinins in rabbits induced by Mycoplasma pneumoniae, Listeria monocytogenes or Streptococcus MG.


Morphological determinants in O2-transfer across the human and Rhesus hemochorial placenta.


Placental function and oxygenation in the fetus.


Threshold values of oxygen deficiency leading to cardiovascular and brain pathological changes in term monkey fetuses.


Experimental models for in vivo and in vitro investigations on placental hemodynamics and oxygen supply to the fetus.


Existing anatomical parameters and the need for further determinations for various tissue structures.


Effect of carbonic anhydrase on the facilitated diffusion of CO2 through bicarbonate solutions.


Time course of weight increase of anterior pituitary and thyroxine binding to adenopituitary proteins after a single administration of estrogen.


The detection of LH activity in sheep urine by radioimmunoassay.


Serial passage of 3 strains of avian infectious bronchitis virus in African green monkey kidney cells (VERO).


Role of desquamated epithelial cells in transmission of Marek's disease.


Isolation of type-A influenza viruses from imported exotic birds.


Notes on reproduction in captive squirrel monkeys (Saimiri sciureus).


Evaluation of the water incentive method of social dominance measurement in primates.


Effect of virus pathogenicity on antibody production in Marek's disease.


Applicability of heat-stable deoxyribonuclease assay for assessment of staphylococcal growth and the likely presence of enterotoxin in cheese.


Blood groups, red cell enzymes and serum proteins of baboons and vervets.


Effect of pesticides on the methylene blue reduction test in milk.


An easy method for making cutters to use in the agar-gel diffusion technique.


Are all those lab tests really worth it?


Summary of recent studies in Japan on methyl mercury poisoning.


Studies on the mechanism of learning. II. On the ionic nature of the dendritic action potentials and mescaline spikes.


Observations on the effect of changes in arterial oxygenation on adenosine induced coronary vasodilation.


The effect of glyceryl trinitate on coronary collateral flow--a preliminary report.


Local oxygen tension and blood flow in the normal and chronic ischemic myocardium in the anesthetized dog.


Coronary artery reperfusion: early effects on coronary hemodynamics.


Effect of incubation time on folate values.


Simple pyrazinamidase and urease tests for routine identification of mycobacteria.


Clarification of sonic lysates of Mycobacterium tuberculosis.


Isolation of tuberculin skin test reactive proteins from heated culture filtrate of Mycobacterium tuberculosis H37Rv.


Modern concepts in the management of surgical diabetic patients.


Polycystic jaws in patients with cleft lip and palate.


Comparative observations on intertubular lymphatics and the organization of the interstitial tissue of the mammalian testis.


Periodic thermal stress: optimal spacing predicted by a simple set-back model of cell division synchronization.


Failure of TSH radioimmunoassay to consistently detect mild TSH mediated thyroid stimulation.


Serum triiodothyronine in health and disease.


Serum TSH responses to intravenously and orally administered TRH in man after thyroidectomy for carcinoma of the thyroid.


Mendelian segregation of a mouse antibody idiotype.


Bursal and postbursal stem cells in chicken. Functional characteristics.


Mesenteric lymph as a major source of serum IgA in guinea pigs and rats.


Growth hormone levels in vitamin B6-deficient rats.


Plasma disappearance of radioiodinated dog growth hormone in the dog.


Importance of plasma inorganic phosphate on tissue oxygenation during recovery from diabetic ketoacidosis.


Studies on the conjugation of horseradish peroxidase to Fab fragments.


Micro-hemagglutination tests for detection of native and single-strand DNA antibodies and circulating DNA antigen.


Proceedings: The mechanism of action of prostaglandin F2a on cerebral flow in the baboon.


Differential effects of glycerol treatment on membrane capacity and excitation-contraction coupling in toad sartorius fibres. Changes in membrane capacity and excitation-contraction coupling caused by glycerol movements have been investigated in toad sartorius fibres using a standard glycerol-Ringer solution containing 400 mM glycerol.1. The rates of glycerol movement, in and out of fibres, were determined by measuring diameter changes in single fibres. Glycerol equilibrated across the surface membrane within 20-25 min after changes in extracellular glycerol concentration.2. The reduction in membrane capacity, which occurs when glycerol-loaded fibres are returned to Ringer solution, was slower than, and not dependent on, changes in fibre volume.3. To obtain the maximum reduction in membrane capacity, it was necessary to expose fibres to glycerol-Ringer for 50-60 min and then to return them to Ringer for at least 60 min. If preparations were not kept in Ringer for the full 50-60 min, the reduction in membrane capacity could be partially or completely reversed by returning the fibres to glycerol-Ringer.4. When fibres were exposed to glycerol-Ringer there was an initial transient contracture: twitches and K contractures were rapidly inhibited, and then slowly recovered over the next 40-50 min. In some preparations, eventual potentiation of twitches was seen.5. When returned to Ringer solution after 60 min in glycerol-Ringer, preparations lost twitches and K contractures within 5-10 min. The time course of this effect was very similar to the time course of the recovery of the normal volume after the initial swelling.6. The briefer the exposure to the glycerol-Ringer, the slower the loss of twitches and K contractures on return to Ringer. In contrast to the loss of membrane capacity, the lesion of excitation-contraction coupling was essentially irreversible. Exposure times as brief as 5 min were eventually effective in producing paralysed fibres which, however, still responded to caffeine.7. The differences in the glycerol load-times required to produce decoupling of excitation and contraction, and disconnexion of the transverse tubules, show that the two effects are separable and that the first does not depend on the second.8. It is proposed that the two lesions obtained in glycerol-treated fibres may be related to distension of the transverse tubular system. It is shown in an Appendix that the outward movement of glycerol from sarcoplasm to transverse tubules would be expected to produce some early swelling of the tubules and this is related to the loss of contraction. Furthermore, much greater swelling would occur if a slow-loading compartment (presumed to be the sarcoplasmic reticulum) dumped glycerol into the transverse tubules: it is thought that this is related to the disconnexion of the transverse tubules.


Proceedings: Reactive hyperaemia in the dog heart: assessment of post-hyperaemic coronary vascular reactivity and of the effects of temporarily restricting arterial inflow.


Proceedings: Extrinsic and intrinsic hysteresis of intrapulmonary bronchi in situ.


Dexamethasone in the management of kerosene pneumonia.


A method of fixing rat testis for light and electron microscopy.


Growing and embedding fern gametophytes for autoradiographic studies.


Regulation of transport systems: a means of controlling metabolic rates.


Control of photosynthetic carbon metabolism.


Bladder neck obstruction in young male patients.


Cecovesicovaginal fistula.

Cecovesicovaginal fistula.


Effect of pH, sodium chloride, and sodium nitrite on enterotoxin A production. The combined effects of pH, sodium chloride, and sodium nitrite were studied by using a dialysis sac technique in brain heart infusion broth. Growth and enterotoxin A production by Staphylococcus aureus strain 100 were found to decrease with the addition of sodium nitrite, with a decrease in pH from 7.0, and with an increase in sodium chloride concentration. The significance of these results is discussed in relation to cured meats.


Field evaluation of the gel diffusion test for the diagnosis of ram epididymitis caused by Brucella ovis. A comparison was made with the microslide gel diffusion technique, the complement fixation test, cultural isolation, and clinical examination in detecting ram epididymitis caused by Brucella ovis. The results of the gel diffusion method are shown to be similar to those obtained by the complement fixation test and isolation of B. ovis from cultures of semen. The technique offers a reliable diagnostic method adaptable to field use in controlling ovine brucellosis and is more practical.


Multiplication of nosocomial pathogens in intravenous feeding solutions. A major problem in total parenteral nutrition is sepsis, particularly that caused by Candida. Studies of four solutions, a casein hydrolysate, a fibrin hydrolysate, and two crystalline amino acid solutions, show that the protein hydrolysate solutions appear to be highly selective for Candida over bacteria, whereas the crystalline amino acid solutions are not. These findings suggest that the crystalline amino acid preparations may offer a partial solution to the infection problem by minimizing the contribution of the solution as a reservoir for organism multiplication, because they retard the growth of both bacteria and Candida.


Assimilation of hydrocarbons by Pseudomonas strains isolated from human clinical specimens. Twenty strains of Pseudomonas isolated from human clinical specimens on routine laboratory media, without hydrocarbon enrichment and unselected for their growth on hydrocarbons, were tested for their ability to utilize a series of eight n-alkanes and two 1-alkenes as a sole carbon and energy source for growth. Hydrocarbon assimilation does occur with such isolates relative to the chain length and the degree of saturation of the hydrocarbon. The data presented show that all 16 stains of Pseudomonas aeruginosa studied grew readily on dodecane through hexadecane and on 1-hexadecene. In addition, most strains of this species grew on undecane and 1-dodecene after prolonged incubation. There was a long lag period, usually a minimum of 4 days, before onset of growth on any hydrocarbon. In no case did hexane or decane support growth. Two strains each of P. maltophilia and P. stutzeri were unable to grow on any of the hydrocarbons tested. Hexane in concentrations above 1% (vol/vol) is bactericidal toward the Pseudomonas inoculum. It is toxic even to cells utilizing different hydrocarbon for growth. The addition of 1% hexane to 1% (vol/vol) hexadecane markedly prolonged the lag phase of P. aeruginosa utilizing the hexadecane for growth.


Comparison of three culture media for isolation of Mycobacterium tuberculosis: a 6-year study. Among 2,648 specimens positive on culture for Mycobacterium tuberculosis over a 6-year period, 82% grew on Lowenstein-Jensen medium (LJ), 79% on American Trudeau Society (ATS), and 56% on Middlebrook 7H10 (7H10). When these commercial culture media were compared in regard to the number of acid-fast bacilli seen on the original smears, LJ cultures were found to have the highest isolation rates for each smear category, and 7H10 had the lowest rates. Comparing the media from the aspect of number of mycobacterial colonies produced, LJ and ATS had the highest average colony counts, followed by 7H10. These findings were relatively constant over the 6-year period. One possible reason for the low positive rate of 7H10 was the lack of CO(2) enrichment.


Scanning electron microscopy of bacterial colonies. A technique is described for observing bacterial colony growth. Bacillus cereus, B. subtilis, and B. cereus var. mycoides were grown on strips of dialysis membrane layered on nutrient agar. Microcolonies of the organisms on strips were fixed in Formalin vapor in situ; the strips then were removed from the agar and secured to scanning microscope specimen stubs without markedly disturbing the cellular arrangement. Scanning electron micrographs clearly depict morphology of individual cells, as well as the spatial orientation of cells within the colony. This technique is reproducible, adaptable, and simple.


Carbohydrate metabolism in lactic streptococci: fate of galactose supplied in free or disaccharide form. Phosphorylation of free galactose by lactic streptococci was mediated by an adenosine triphosphate (ATP)-dependent kinase. The phosphoenolpyruvate (PEP) phosphotransferase system (PTS) was involved to a limited extent in transport of the sugar. The conversion of free galactose to glucose also was demonstrated, and uridine diphosphogalactose-4-epimerase was demonstrated to account for this change. Galactose, supplied as lactose, was phosphorylated during transport by means of the PTS with PEP as the phosphate donor. Data also indicated that galactose derived from lactose was catabolized by the glycolytic pathway. Results showed the participation of ATP or PEP, or both, in the phosphorylation of five growth sugars for lactic streptococci, namely, galactose, glucose, lactose, maltose, and mannose. Free galactose was phosphorylated exclusively by ATP except when cells were grown on galactose; in this case, slight involvement of PEP in phosphorylation also was noted. Lactose phosphorylation was much more effective with PEP except when cells were grown on lactose, in which case ATP was equally effective. Glucose was phosphorylated to about the same degree by either ATP or PEP.


Population changes in enteric bacteria and other microorganisms during aerobic thermophilic windrow composting. Composting of wastes from swine feeding operations was studied. The effects of the frequency of turning the wastes and addition of straw to improve the physical structure were studied to determine the most effective technique to rapidly increase the temperature and, consequently, destroy coliforms and Salmonella. Four different treatments were studied; the results showed that, with addition of 5% (wt/wt) straw and mechanical turning of the compost 20 times per week, the temperature reached 60 C within 3 days and enteric bacteria were destroyed within 14 days.


Localized epidermolysis bullosa. Report of two cases and evaluation of therapy with glutaraldehyde.


Letter: Photosensitive lichenoid eruption associated with demeclocycline.


Azotobacter vineland II RNA polymerase. XI. Effect of transcription on rifampicin binding.


Isolation of a replication-complex from eukaryotes.


Antigenic homology of eukaryotic RNA polymerases.


A new polypeptide associated with RNA polymerase from Bacillus subtilis during late stages of vegetative growth.


Effect of age of the rat on induction of acetylcholinesterase of the brain by 17 beta-estradiol.


Proceedings: Prostaglandin generation maintains the smooth muscle tone of the rabbit isolated jejunum.


Proceedings: Effect of oxypertine on anxiety-induced behaviour in baboons.


A comparative study of the accessory ganglia of Axenfeld.


Effects of feeding and fasting on the insulin secretory response to glucose and sulfonylureas in intact rats and isolated perfused rat pancreas.


Sex-linked blood-glucose pattern in rats under varying test conditions.


Immunoglobulin classes in mammalian species identifed by cross-reactivity with antisera to human immunoglobulin.


Characterisation of an allergen extracted from Ascaris suum. Determination of the molecular weight, isoelectric point, amino acid and carbohydrate content of the native allergen.


The structure of the zonula occludens. A single fibril model based on freeze-fracture. Replicas of freeze-fractured toad urinary bladder and gallbladder were analysed in an attempt to determine the fracturing properties and structure of the zonula occludens (tight junction). Chalcroft and Bullivant have proposed that the junction has a double set of fibrils with one set associated with each of the adjacent cell membranes. However, the fracturing pattern that is observed might also result from only a single set of fibrils which is shared by the adjacent membranes if fracturing occurred around either side of the fibrils. These two models predict quite different structures at regions of the junction where tranl sitions are made between face A and face B. The relative heights of face A and face B and the shape of the transition from face A to face B do not agree with that expected according to the two fibril model but agree exactly with that expected if only a single set of fibrils existed. Further evidence for the single fibril model is derived from fractures of the mucosa membrane which cross the junction to the membrane of the adjacent cell without deflection. Such fractures reveal a single ridge which appears to be identical to the juxtaluminal fibril of the junction. In addition, small ridges are occasionally found in place of the grooves on face B which, although not consistent with the double fibril model, is expected if the single fibril model were correct. Although alternative explanations might account for these observations, we believe that the simplest and most consistent explanation is that the zonula occludens fractures as would be expected of a single set of fibrils shared by adjacent cells.


The effect of glutaraldehyde on catalase. Biochemical and cytochemical studies with beef liver catalase and rat liver peroxisomes.


Cryoprotectant-induced redistribution of intramembranous particles in mouse lymphocytes. This study demonstrates, by freeze fracture, clustering of intramembranous particles caused by cryoprotectant treatment of intact unfixed mouse lymphoid cells. Both T and B cells react in a similar fashion, while similar clustering of particles is not observed in some other cell types. The intramembranous particles can be aggregated by incubating unfixed cells in glycerol or dimethylsulfoxide (DMSO) before freezing. The aggregation phenomenon is dependent on the length of time of exposure and the concentration of the cryoprotectants. Further, the cells remain viable and the cryoprotectant-induced clustering is completely reversible. Prefixation of glycerol-treated cells with glutaraldehyde prevents the formation of these particle clusters, and unfixed nonglycerinated cells show no clusters. Thin sections of cells exposed to glycerol or DMSO without previous fixation exhibit bizarre membrane alterations and numerous other degenerative changes. These observations stress the importance of prefixation of lymphoid cells before exposure to glycerol or DMSO, as well as indicate that the membrane characteristics of certain cell types may be probed by glycerol treatment of unfixed cells.


Studies on the virulence of Neisseria gonorrhoeae. II. Relation of susceptibility to antibiotics and resistance to phagocytosis by polymorphonuclear leukocytes.


Synthesis and biological activities of substituted glycyrrhetic acids.


Nucleolar and perichromosomal RNA synthesis during meiotic prophase in the mouse testis. The transcriptional activity during meiotic prophase in the mouse testis is studied with light microscopy and high-resolution autoradiographic techniques using [(3)H]uridine as a labeled precursor. In the present study, two types of RNA synthesis are detected during meiotic prophase: an extranucleolar RNA synthesis of perichromosomal localization and a nucleolar RNA synthetic activity. In some of the autosomes and close to the basal knobs, the activity of the nucleolar organizers is evidenced by the incorporation of [(3)H]uridine into nucleolar masses from zygotene on and at earlier labeling times. The evolution of nucleoli and the formation of a nucleolus attached to the sex pair are described during the different meiotic stages. Perichromosomal labeling, from leptotene on, reaches a maximum during middle pachytene and falls progressively to a low level at longer incorporation times. Sertoli's cell, the most active RNA synthetic cell in the seminiferous epithelium, rises to a maximum of labeling and drops at earlier times compared with the meiotic prophase cells. The condensed sex chromosomes show some scattered silver grains especially at middle pachytene. The axial chromosome cores and synaptonemal complexes are devoid of silver grains during the meiotic prophase. The observations suggest that a control mechanism operates during meiotic prophase to regulate transcriptional activity in the sex chromosomes and to provide differential RNA synthesis in autosomal bivalents at various stages of prophase and within certain segments of the chromosomes.


Current techniques in assessment of protein nutritional status in the community.


Antiparasitic 5-nitrothiazoles and 5-nitro-4-thiazolines. 4.


Synthesis of 2-oxygenated glycyrrhetic acid derivatives.


Folic acid analogs. Modifications in the benzene-ring region. 4. 3'-Ethyl- and 3'-isopropylfolic acids.


Syntheses and biological activities of 7-ethyl-8-bromo-10-(1'-D-ribityl)isoalloxazine and 7-bromo-8-ethyl-10-(1'-D-ribityl)isoalloxazine, analogs of riboflavin.


Synthesis and biological evaluation of isofolic acid.


Experience with Björk-Shiley valvular replacement. A preliminary report.


Ribosomal RNA cistrons in Euglena gracilis.


Prostaglandin inhibition of metabolically induced coronary vasodilation.


Inotropic stimuli and systolic transmembrane calcium flow in depolarized guinea-pig atria.


Metabolism of diazoxide in man and experimental animals.


Pharmacodynamic interactions among gluco-, mineralo- and glucomineralocorticoids, pregnenolone-16alpha-carbonitrile and various drugs.


Plasma and erythrocyte zinc levels in protein-calorie malnutrition.


Endotoxemia in the Rhesus monkey: alterations in host lipid and carbohydrate metabolism.


The absorption, metabolism and excretion of disodium cromoglycate in nine animal species.


The relationship between inhibition of delta-aminolevulinic acid dehydratase by lead and lead mobilization by ethylenediamine tetraacetate (EDTA).


Intraepithelial parasitism as an infection mechanism in human paracoccidioidomycosis (South American blastomycosis).


Human intracranial atherosclerosis. An ultrastructural study of atheromatous plaques.


Serum immunoglobulin levels in atomic bomb survivors in Hiroshima, Japan.


Observations on asymptomatic infections with Neisseria meningitidis.


Aldolase of lactic acid bacteria: a case history in the use of an enzyme as an evolutionary marker.


Computer morphometrics in craniofacial biology.


Increase in the longevity of adult Drosophila melanogaster kept in permanent darkness.


The thyroid and age.

The thyroid and age.


Editorial: Renal failure in tropical diseases.


Regeneration of the attachment apparatus on pulpless teeth denuded of cementum in the rhesus monkey.


Reduction of nitrogenous oxides by microorganisms.


Potatoes and birth defects: summary.

Potatoes and birth defects: summary.


Investigations into the teratogenic potential of imperfect potatoes.


Are potatoes teratogenic for experimental animals?


The origin of immunoglobulin-G in bovine tears.


Analysis of C1 subcomponents in cell culture media of HeLa cells and diploid human fetal lung fibroblasts.


Scanning the radiation field of a 25-MeV medical betatron using a new uniformity check system.


Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes.


Moraxella, Acinetobacter, and the Mimeae.


Influence of certain pesticides on microorganisms. Influence of DDT on the synthesis of the particular amino acids in Chlorella vulgaris and Anabaena cylindrica.


Quantitative and qualitative aspects of the plasma protein binding of carbenoxolone, an ulcer-healing drug.


Plasma proteins in the semen of bulls.

Plasma proteins in the semen of bulls.


Interaction of drugs with thyroid hormone binding sites.


Blood group homologues in orangutans and gorillas of the human Rh-Hr and the chimpanzee C-E-F systems.


Feeding levels and feeding sites of red colobus (Colobus badius tephrosceles) in the Gombe National Park.


The distribution of the white-collared mangabey, Cercocebus torquatus, in Nigeria.


Changes in the sexual behaviour of male rhesus monkeys (M. mulatta) at puberty. Comparisons with the behaviour of adults.


Advances in rehabilitative care lag behind progress in managing episodic disease.


In vitro fertilization of human ova and blastocyst transfer. An invitational symposium.


Pyrolysis-gas-liquid chromatography of fungi: numerical characterization of species variation among members of the Aspergillus glaucus group.


Nurse autonomy and accountability in organized nursing services.


Concomitant occurrence of neurofibromatosis and carcinoid of the intestine.


The histocompatibility complex of rhesus monkeys. I. Serology and genetics of the RhL-A system.


Scanning electron microscope observations on the spore of Nosema apis Zander.


Carcinoma of the ovary. Radiation therapy experience.


Mammary nodular hyperplasia in intact rhesus monkeys. Clinical and pathological findings of subcutaneous nodules in the breast regions of 2 untreated control intact female Rhesus monkeys in a long-term study of an oral contraceptive steroid are described.  1 nodule, which was excised early in the study, showed changes characteristic of nodular mammary hyperplasia; no features of neoplasia were present.  Some of the nodules were transient.  None of the nodules showed a progressive growth pattern during repeated clinical examination over 17-20 months.


Besnoitia bennetti in two Mexican burros.


Prognosis of depressed skull fracture.

Prognosis of depressed skull fracture.


Letter: Spinal anesthesia and ephedrine in pregnant monkeys.


The presence and possible significance of cross-reactive antigens in Rhizobium--legume associations.


Proceedings: Netherlands Society for Microbiology meeting at Utrecht on 2 May 1973. Chemotaxis in microorganisms.


Proceedings: Netherllands Society for Microbiology meeting at Utrecht on 2 May 1973. Environmental effects on bacterial wall synthesis.


Evaluation of 167Tm HEDTA as a bone scanning agent in humans and its comparison with 18F.


Intra-uterine infection and cord immunoglobulin M. II. Clinical analysis of infants with elevated cord serum immunoglobulin M. Cord blood immunoglobulin M was measured in 3474 consecutive newborn infants. A group of 147 infants with elevated IgM values (>/=19.0 mg./100 ml.) were compared with 92 unselected newborn infants with normal IgM values. One infant with clinically unsuspected congenital rubella was detected in the study group while no cases of intra-uterine infection were found among the controls. A greater proportion of mothers in the study group had a history of viral infection. The study group also contained a larger number of mothers who might be considered to be at greater risk of infection with agents known to cause intra-uterine disease. Follow-up studies at 6 months of age revealed no differences between the two groups aside from an increased incidence of minor motor abnormalities in the study group. While it is recognized that infants with cord blood IgM levels truly in excess of 30 mg./100 ml. may represent a high-risk group with respect to proved or subclinical intra-uterine infection, it is concluded that routine cord blood screening for elevated IgM values is not a high-yield procedure for the detection of intra-uterine infection in our population.


Strain-dependent teratogenic effects of 1-ethyl-1-nitrosourea in inbred strains of mice.


A comparison of three in vivo assays for cell tumorigenicity.


The reaction between carcinoembryonic antigen and concanavalin A.


Differing methodology and equations used in quantitating immunoglobulins by radial immunodiffusion--a comparative evaluation of reported and commercial techniques.


Letter: Commercial immunodiffusion plates.


Letter: Non-narcotic buffer for hemoglobin electrophoresis.


Pharmacologic weakening of extraocular muscles.


Injection technique for indocyanine green and sodium fluorescein dye angiography of the eye.


A correlation of anatomic and physiologic parameters in the ureter.


The relationship of epididymitis to gonorrhea.


Snake venom toxins. The purification and amino acid sequence of toxin TA2 from Dendroaspis angusticeps venom.


Nutritional status of children at Namitambo, Malawi.


Influence of maximal vasodilatation on glucose and sodium blood-tissue transport in canine heart.


Utilization of fructose by a unicellular blue-green alga, Anacystis nidulans.


Immunosuppressive activity of a heat-stable factor associated with interferon.


Mechanisms of ankle injury.

Mechanisms of ankle injury.


Effect of soft tissues on radiographic measurements of the head.


Injuries of the ankle in children.

Injuries of the ankle in children.


Ankle injuries in athletes.

Ankle injuries in athletes.


Indications for fusion of the ankle.

Indications for fusion of the ankle.


Pathologic response of gingiva to an amino acid increase.


The use of peripheral veins for alimentation in paediatric patients.


Enteric duplications.

Enteric duplications.


A human IgA-myeloma protein with anti-streptococcal hyaluronidase (ASH) activity.


Bovine fibrinogen-induced platelet factor 3 and acid phosphatase availability in human platelets.


Pulp reaction to a polycarboxylate cement in monkeys.


Chlorinated hydrocarbons in the Sargasso sea atmosphere and surface water. Polychlorinated biphenyls (PCB), DDT, and chlordane concentrations were measured in air sampled from a tower on the south shore of Bermuda and in Sargasso Sea surface water approximately 80 to 320 kilometers south of Bermuda. The atmospheric chlorinated hydrocarbons appeared to be gaseous, and the DDT concentration was two orders of magnitude higher than previously reported particulate values. The PCB and DDT were enriched in the surface microlayer (150 micrometers) relative to their concentrations in water at a depth of 30 centimeters. Atmospheric residence times for PCB and DDT of 40 to 50 days, calculated from the concentrations in the air and water, are 20 times shorter than values previously estimated for DDT from rainfall and DDT production data.


Nutritional significance of symbiotic bacteria in two species of hemoflagellates. Symbiote-free strains of Blastocrithidia culicis and Crithidia oncopelti, obtained by chloramphenicol treatment, were compared nutritionally with normal, symbiote-containing strains. The symbiotic bacteria spare the flagellates requirements for exogenous hemin and for other nutritional factors present in liver extract.


Cerebroside antibody inhibits sulfatide synthesis and myelination and demyelinates in cord tissue cultures. Antiserum to cerebroside was prepared in rabbits by injection of cerebroside together with bovine serum albumin in complete Freund's adjuvant. When applied to cultures of embryo mouse spinal cord at explantation, this antiserum inhibited sulfatide synthesis and myelination; when applied to myelinated cultures it inhibited sulfatide synthesis and produced demyelination. Complement fixation assays also show antibody to cerebroside in serums from rabbits with experimental allergic encephalomyelitis induced by injection of whole white matter. Absorption of such serum with cerebroside abolishes the inhibiting and demyelinating activities.


Leptospirosis in Philippine monkeys.

Leptospirosis in Philippine monkeys.


Ecological distribution of blood parasites in some arboreal rodents.


Sarcocystis from rats of Central Java.

Sarcocystis from rats of Central Java.


Relationship between liver weight and body weight in normal monkeys and dogs.


Distribution and metabolism of DDT, carbaryl and methyl parathion in S-and R-strains of Spodoptera littoralis (Boisd).


The administration of monosodium L-glutamate to neonatal and pregnant rhesus monkeys.


Serum immunoglobulin and complement levels relative to renal homotransplant rejection and patient survival. A retrospective study.


Infarction of spinal cord and medulla oblongata caused by fibrocartilaginous emboli. Report of a case.


Ultrastructural and biochemical study of benign ganglioneuroma.


"Vaso-muscular mediadysplasia" of the arteria vertebralis.


Case report: Primary (autoimmune?) parathyroiditis.


Effects of insulin on glucose metabolism in vascular and intestinal smooth muscle.


Standardization of test-negative cream for Brucella milk ring test.


Efficacy of EDTA-tris-lysozyme lavage in the treatment of experimentally in induced Pseudomonas aeruginosa cystitis in the dog.


Myocardial contractility in conscious rhesus monkeys: a method for long-term study.


Parenteral nutrition by total intravenous feeding.


Childhood, juvenile, and adult cerebral lipidoses. Are these different nosological entities?


Toxic and immunological differences among lipopolysaccharides from Thiobacillus ferrooxidans grown autotrophically and heterotrophically.


Biological production of 5-methylthioribose.


The physiology of teichoic acid deficient staphylococci.


The effect of thyroxine and triiodothyronine on bacterial growth.


The appearance of some blood-bourne substances during human pregnancy.


Alkaline phosphatase of Pseudomonas aeruginosa: the mechanism of secretion and release of the enzyme from whole cells.


Solitary mastocytoma of infancy.

Solitary mastocytoma of infancy.


The problem of bacteriologically contaminated infant formulas in a newborn nursery.


Plasma norepinephrine and epinephrine in untreated diabetics, during fasting and after insulin administration.


Lipid and enzyme changes in the blood and liver of monkeys given butylated hydroxytoluene and butylated hydroxyanisole.


The formation of carcinogenic nitroso compounds from nitrite and some types of agricultural chemicals.


Studies on the uridine diphosphate-galactose: lipopolysaccharide galactosyltransferase reaction using a fatty acid mutant of Escherichia coli.


Protein synthesis with messenger ribonucleic acid fractions from membrane-bound and free liver polysomes. Translation characteristics of liver polysomal ribonucleic acids and evidence for albumin production in a messenger-dependent reticulocyte cell-free system.


Use of benzamidine to prevent the destruction of thyrotropin-releasing hormone (TRH) by blood.


Thyroglobulin defect in a human congenital goiter.


Effect of synthetic luteinizing hormone-releasing hormone (LH-RH) on the release of gonadotropins in hypophyso-gonadal disorders of children and adolescents. 3. Hypopituitarism.


Role of central monitoring of efference in short-term memory for movements.


Secondary hypopituitarism: evidence for continuing regulation of hormone release.


Induction of leukemoid disease in mice by Mycoplasma fermentans.


Isolation of cores from hepatitis B Dane particles.


Correspondence: Culture of Neisseria gonorrhoeae.


Congenital toxoplasmosis. Fetal synthesis of oligoclonal immunoglobulin G in intrauterine infection.


Activation of hepatic microsomal glucuronyl transferase by bilirubin.


Block of oxytocin-induced parturition and oviposition by prostaglandin inhibitors.


Clinical pharmacology of carbenicillin and gentamicin in the neonate and comparative efficacy with ampicillin and gentamicin.


The influence of hexachlorophene scrubs on the response to placebo or penicillin therapy in impetigo.


Crystal formation: a new complication of total parenteral nutrition.


Increase in the plasma concentration of free tryptophan caused by probenecid in humans.


Effect of gold salts and antimalarials on the rheumatoid factor in rheumatoid arthritis.


Estriol metabolism in the baboon: analysis of urinary and biliary metabolites.


G.C. culture evaluation in a community health center. A study of 870 cases.


Action of some metal ions, EDTA, and heated milk on the milk-clotting process of Penicillium citrinum rennin-like enzyme.


The role of fat as a calorie course in parenteral nutrition.


Heavy metals--toxicity and environmental pollution.


A survey of naturally occurring chelating ligands.


Clinical effectiveness of lividomycin on urinary tract infections: evaluation with double-blind method.


Halofenate: an effective hypolipemia- and hypouricemia-inducing drug.


Use of lorazepam in treating patients with neurotic and somatized psychovegetative symptoms.


Molindone: a crossover evaluation of capsule and tablet formulations in severely ill schizophrenic patients.


Amoxapine and imipramine: a double-blind study in depressed patients.


A method of pooling data of different rating scales.


Homoeosis in Drosophila. I. Complementation studies with revertants of Nasobemia. A number of homoeotic mutants have been localized to the proximal right arm of chromosome 3 of Drosophila melanogaster. These include seven alleles of Antennapedia (Antp), which is associated with a transformation of antennae into legs; Nasobemia (Ns), which causes the same phenotype as Antp but was considered by Gehring (1966) not to be an allele; and three genes causing a transformation of second and third legs into first legs: Extra sex comb (Scx), Polycomb (Pc), and Multiple sex comb (Msc.). The alleles of Antp and Scx share a common recessive lethal effect, and Pc maps 0.2-0.3 units to the left of Scx.-In the present investigation, rearrangements associated with the reversion of Ns suggest that its cytological location is in or just distal to salivary chromosome doublet 84B1-2. Although Ns is viable when homozygous, four of its revertants share a common recessive lethal effect. These revertants fail to complement the recessive lethality of Antp(B) and Scx. Furthermore, they show a complex pattern of functional interaction with Pc and with Humeral (Hu), a dominant mutation associated with a rearrangement with one breakpoint just distal to 84B1-2. Finally, analysis of a revertant of Msc indicates that Msc is also located very close to 84B1-2. It is concluded that Ns and Scx are alleles of Antp. Pc shows many functional similarities to the Antp locus, but is probably not allelic. Evidence is presented that these dominant homoeotic genes are neomorphic in nature.


The interaction of two complex loci, zeste and bithorax in Drosophila melanogaster. It has been found that certain alleles of the zeste locus (z(a) 1-1.0) have no phenotype of their own, but interact with certain alleles at the bithorax locus (bx 3-58.8). This interaction takes the form of an enhancement of the homeotic bx phenotype to a more extreme form-i.e., the metathorax is transformed into mesothorax in varying degrees depending on the bx allele used. This enhancement is somewhat reminiscent of the transvection effect described by Lewis (1954). The characterization of the interaction thus far has shown that the enhancement only effects bx alleles which arise spontaneously, whereas the origin of the z(a) allele is unimportant. The gene claret nondisjunctional was used for the production of gynandromorphs which showed that the enhancing ability of z(a), like the eye pigment change caused by z, is autonomous. The enhancement of one specific allele (bx(34e)), which is temperature-sensitive, has allowed a delineation of the temperature-sensitive period of the bithorax locus to a period extending from the middle of the second larval instar to the middle of the third larval instar. These results, as well as those of other enhancer and suppressor systems in Drosophila, have revealed the possibility of the involvement of heterocyclic compounds in the control of cell determination and fate in Drosophila melanogaster.