Tests of disinfection by heat in a bedpan washing machine. Tests of effectiveness of disinfection of metal and polypropylene bedpans were made in a washer fitted with a steam generator. Broth cultures of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, or Streptococcus faecalis (approximately 4 x 10(8) organisms) were sealed in lengths of capillary tubing and attached to the surface of the pans. In other tests, pans were contaminated with an artificial soil containing Str. faecalis (10(8) organisms/ml). In both series of tests, counts of surviving organisms were made at the end of the washing and disinfection cycle. The tests using capillary tubes showed that the Gram-negative bacilli were effectively killed, but not necessarily Gram-positive cocci. However, when incorporated in standard soil, Str. faecalis was killed or removed during the cycle. The results indicate that the disinfection process was effective for metal bedpans, but less so for polypropylene. Possible disadvantages and modification of the machine are suggested.


Alteration of classically conditioned heart rate by operant reinforcement in monkeys.


Effects of sequential preoptic and mammillary lesions on male rat sexual behavior.


Acid etching: questions and answers.

Acid etching: questions and answers.


Behavioral diversity in ten species of nonhuman primates.


The health care revolution (or dental practice will never be the same).


Mouth guards in contact sports.

Mouth guards in contact sports.


(Need) divided by x minus (Demand) divided by xR equals orthodontist glut.


"Sorry doctor, but I disagree".

"Sorry doctor, but I disagree".


Correction of trauma from occlusion in treatment of periodontal disease.


Ability to function with full dentures, natural dentition, and no dentition.


Factors affecting L-alanine induced germination of Bacillus spores.


[A comparison of the pathogenicity of two strains of Schistosoma mansoni in Cercopithecus aethiops monkeys in the Sudan].


A cytochemical approach to the lipid composition of mitochondrial matrix granules in brown adipose tissue and liver.


First cases of diphtheria in Aruba (Dutch West Indies).


Contribution to knowledge of factors participating in virulence of Listeria monocytogenes. II. Study of the chemical nature of complex Ei.


Isolation of type - specific substances from Corynebacterium diphthermiae cells.


Shigella surveillance in Czechoslovakia.


The phases of Coxiella burneti and its immunosorbents.


Total viable count of microorganisms in the infected dental pulp.


Allergens of mammalian origin. III. Properties of a major feline allergen.


Antibodies to proteins dissolved in sodium dodecyl sulfate.


Cross-idiotypic specificity of rat antibodies to group A streptococcal carbohydrate.


Anti-idiotypic antibody in rat transplantation immunity. I. Production of anti-idiotypic antibody in animals repeatedly immunized with alloantigens.


Mononuclear cell chemotactic activity of kallikrein and plasminogen activator and its inhibition by C1 inhibitor and alpha 2-macroglobulin.


Molecular analysis of the reaction of C9 with EAC1-8: reaction of C9 with EAC1-8.


Expression of IgG allotypes in human lymphoid tumor lines serially transplantable in the neonatal Syrian hamster.


Fate of polychlorinated biphenyls, metals, and other elements in papers fed to lactating cows.


Cellular immunity to Leishmania donovani. I. The effect of T cell depletion on resistance to L. donovani in mice.


Photography of faint immunodiffusion zones.


A pathologic IgM occurring in serum as pentamer, dimer, and monomer.


A controlled quantitative study of parotid salivary secretory IgA-globulin in normal adults.


Study of adenovirus hexon antigen--antibody reactions by single radial diffusion techniques.


The use of peroxidase-labelled antiglobulin for ultrastructural localization of tissue antigens reacting with serum antibodies.


Radioimmunoassay techniques for detecting naturally occurring viral antibody in human sera.


Staphylococcal toxic epidermal necrolysis (TEN): the expanded mouse model.


Histidase activity in rat liver and epidermis.


Chromatographic fractionation and partial characterization of acid phosphatase in guinea-pig epidermis.


Serum immunoglobulin in scabies.

Serum immunoglobulin in scabies.


In vitro studies of the action of antibiotics on Rickettsia prowazeki by two basic methods of cell culture.


Chute-hydropulping waste disposal system: a reservoir of enteric bacilli and pseudomonas in a modern hospital.


The case for gonococcal serology.

The case for gonococcal serology.


Pharyngo-cutaneous fistulae in totally laryngectomized patients.


Why accelerators in radiation therapy?

Why accelerators in radiation therapy?


Breast cancer management.

Breast cancer management.


The involvement of lactoferrin in the hyposideremia of acute inflammation. The hyposideremia of inflammation was found to be based on a three-step mechanism involving lactoferrin, the iron-binding protein from the specific granules of neutrophilic leukocytes. (a) Lactoferrin is Released from Neutrophils in an Iron-Free Form. When phagocytosis was induced in neutrophils by zymosan or bacteria, lactoferrin was recovered in the incubation medium together with other constituents of the specific granules, such as alkaline phosphatase and lysozyme. Lactoferrin extracted from leukocytes was able to bind the amount of iron corresponding to its theoretical iron-binding capacity. After injection of endotoxin into rats, lactoferrin was detected in various tissues where it was normally absent, or in the plasma when the reticuloendothelial system (RES) had previously been blocked by injections of India ink or aggregated albumin. (b) Lactoferrin is Able to Remove the Iron from Transferrin. Significant exchange of iron from transferrin to lactoferrin was observed in vitro only at a pH below 7.0 or in the presence of a high concentration of citrate. However, the fast elimination of lactoferrin in vivo, when saturated with iron, might account for the observed transfer of iron to endogenous or administered apolactoferrin. Intravenous injection of human apolactoferrin into rats caused a marked decrease of the plasma iron level. The kinetics of this process, as well as controls with other proteins, ruled out the possibility of a secondary inflammatory effect due to phlogogenic contaminants. (c) Fe-Lactoferrin is Taken-up by the RES. By immunofluorescence, lactoferrin was shown to be bound and ingested by monocytes. The rate of elimination of human Fe-lactoferrin injected into rats was particularly fast when compared to that of human apolactoferrin, succinylated Fe-lactoferrin, or other human proteins. Blockade of the RES slowed down the rate of clearance of Fe-lactoferrin and was also found to retard the elimination of endogenous rat lactoferrin released by endotoxin. These experiments suggest the existence of specific receptors for Fe-lactoferrin on the membrane of macrophages.


Further observations on genetic transformation in pseudomonas.


The interaction of IgE with rat basophilic leukemia cells. II. Quantitative aspects of the binding reaction. Previous studies indicated that cultured rat basophilic leukemia cells have surface receptors which bind IgE with high specificity. In this paper we describe some quantitative aspects of the phenomenon. The reaction mechanism appears to consist of a simple reversible binding reaction with k(1) = 9.6 x 10(4) M(-1) sec(-1) and k(-1) 1.6 x 10(-5) sec(-1) at 37 degrees C. The calculated K(A) is therefore 6 x 10(9) M(-1). The activation energy of binding was found to be 7.8 kcal/mol. The number of binding sites/cell varied between 3 x 10(5) to over 1 x 10(6). The binding was insensitive to pH's between 6-8 but at pH 3.0 complete dissociation of bound IgE occurred in approximately 1 min at 0 degrees C leaving the receptors for IgE intact. Ca(++) plus EDTA and Mg(++) plus EDTA produce a fairly marked reduction in binding capacity though these reagents alone produce much smaller effects.


The effect of proflavin, ethidium bromide and an elevated temperature on the appearance of nisin-negative clones in nisin-producing strains of Streptococcus lactis.


Properties of a mutant of Pseudomonas aeruginosa affected in aerobic growth.


Control of macromolecular composition and cell division in the blue-green algae Anacystis nidulans.


Selection of a mutant of Bacillus subtilis deficient in glucose-6-phosphate dehydrogenase and phosphoglucoisomerase.


Comparison of antigenic glycoproteins and glycoprotein receptors of concanavalin A isolated from duck embryo cells infected with Marek's disease virus and a herpes virus of turkeys (strain FC126).


Alterations of the immunological specificity of plasma membranes from cells infected with Marek's disease and turkey herpes viruses.


Photosensitization of cyanophage N-1.

Photosensitization of cyanophage N-1.


Studies to improve fixation of human nerves. 1. Effect of duration of glutaraldehyde fixation on peripheral nerve morphometry.


Studies to improve fixation of human nerves. 2. Effect of time elapsed between death and glutaraldehyde fixation on relationship of axonal area to number of myelin lamellae.


An economic, semiautomatic method for in vitro thyroid function tests.


Response of nitrogen metabolism, plasma amino acids and insulin levels to various levels of methionine infusion in sheep.


Responses of growing lambs to abomasal infusion of corn oil, starch, casein, and amino acid mixtures.


Biological tests of a temporary crown and bridge material.


Studies on host-parasite relationships of Simuliidae with mermithids and microsporidans.


Isolation and partial characterization of exo- and endoantigens of Cooperia punctata cultured in vitro.


Entopolypoides macaci (Babesiidae) in Macaca mulatta.


Immunochemical studies on East Coast fever. I. Partial segregation and characterization of the Theileria parva schizont antigen.


Immunochemical studies on East Coast fever. II. Partial segregation and characterization of the Theileria parva piroplasm antigen.


Pulmonary parenchymal damage complicating intravenous alimentation.


Immunochemical studies on East Coast fever. III. Development of an indirect hemagglutination assay using Theileria parva piroplasm antigen.


Variations in intravenous nutrition in the management of catabolic states in infants and children.


Syntheses and antimicrobial properties of isothiocyanate derivatives.


Letter: Development of a stable sublingual nitroglycerin tablet I: interaction of nitroglycerin with selected macromolecules.


Transport mechanisms of beta-lactam antibiotics across everted rat gut.


Photoelectron spectra, hydrolytic stability, and antimicrobial activity of N-chlorinated piperidines.


Formulation and in vitro evaluation of anti-infective dry foams.


Synthesis and antibacterial activity of 5-nitro-2-furfurylidene p-sulfonyl- and p-sulfamoylbenzoylhydrazides. VII.


Isolation of an acidic glycoprotein from rabbit oviducal fluid and its association with the egg coating.


Calcium and zinc in the endometrium and uterine flushings of the roe deer (Capreolus capreolus) during delayed implantation.


Failure to demonstrate a rôle for the acute release of prolactin at oestrus in the ewe.


A technique for obtaining precise chromosome and bilirubin studies on amniotic fluid in twin pregnancy.


Synthesis and antifolate activity of isoaminopterin.


Pyrimido(4,5-c)isoquinolines. 2. Synthesis and biological evaluation of some 6-alkyl-,6-aralkyl-, and 6-aryl-1,3-diamino-7,8,9,10-tetrahydropyrimido(4,5-c)isoquinolines as potential folate antagnoists 1.


Effect of lipophilic substituents on some biological properties of indoles.


Selective binding of metal ions to macromolecules using bifunctional analogs of EDTA.


Methotrexate analogs. 4.7-Methyl derivatives of methotrexate and dichloromethotrexate. A new synthesis and some biological studies.


Semisynthetic beta-lactam antibiotics. 6. 1 Sulfocephalosporins and their antipseudomonal activities.


Familial thyroxine-binding globulin deficiency: search for linkage with Xg blood groups.


A comparison of three different electron microscopical grade glutaraldehydes used to fix ovarian tissue.


Sporulation-specific translational discrimination in Bacillus subtilis.


Isolation by affinity chromatography of a precursor head protein (P23) of bacteriophage T4.


Electrical properties and diffusion potentials in the gallbladder of man, monkey, dog, goose and rabbit.


Purification and properties of the isolated honeybee Z-disc.


Effects of aminoethylation of cysteine residues on the structural and functional activities of the Escherichia coli 30 S ribosomal proteins.


Characterization of an antiserum against human myeloma cells.


Isolation of papovavirus from brain tumor and urine of a patient with Wiskott-Aldrich syndrome.


On diploid cell lines.

On diploid cell lines.


An in vitro micromethod for testing cytotoxic compounds.


Alveolar macrophage in the burned rat.

Alveolar macrophage in the burned rat.


Treatment of open crushing type of industrial injuries of the hand and forearm: degloving, open circumferential, heat-press, and nail-bed injuries.


Research on tissue culture at the National Cancer Institute, with special reference to the contributions of Dr. Virginia J. Evans.


Bacteriophage PBS2-induced deoxycytidine triphosphate deaminase in Bacillus subtilis. The dCTP deaminase induced by Bacillus subtilis bacteriophage PBS2, whose DNA contains uracil instead of thymine, requires metal ion and thiol activators and has a molecular weight of 125,000. The enzyme displays sigmoidal substrate saturation kinetics and inhibition by dUTP, consistent with the deaminase's proposed role of providing balanced levels of dUTP and dCTP for PBS2 uracil-DNA synthesis.


Bacillus subtilis DNA polymerase III is required for the replication of the virulent bacteriophage phi e. The virulent phage phie of Bacillus subtilis which contains hydroxymethyluracil in its DNA requires host DNA polymerase III for its DNA replication. DNA polymerase III(ts) mutant cells infected with phie at restrictive temperatures do not support phage DNA synthesis. However, phie grows normally both at low and high temperatures in the mutant's parent strain and in spontaneous DNA polymerase III(+) revertants isolated from the mutant strain. Temperature-shift-down experiments with phie-infected cells having thermosensitive DNA polymerase III (pol III(ts)) indicate that at 48 C the thermolabile DNA polymerase III is irreversibly inactivated and has to be synthesized de novo after the shift to 37 C, before phage DNA synthesis can begin. Temperature-shift-up experiments with phie-infected mutant cells show that phage replication is arrested immediately after the temperature shift and indicate that phie requires DNA polymerase III throughout its replication stage.


Transduction in Bacillus subtilis by bacteriophage SPP1. Lysates of the virulent bacteriophage SPP1 were shown to be capable of mediating generalized transduction. Suppressible mutants of this bacteriophage (sus) were capable of transduction at a lower multiplicity of infection than virulent SPP1. Linkage analysis demonstrated that bacteriophage SPP1 transduced segments of the genome equal in size to that transferred by SP10. This bacteriophage should be useful in analyzing the regions of the genome where PBS1 appears to give anomalous results.


Mixed infections of Bacillus subtilis involving bacteriophages SP82 and beta 22. Progeny yields and the synthesis of nucleic acids have been investigated in two strains of Bacillus subtilis mixedly infected with two unrelated phages, SP82 and beta22. When B. subtilis strain 168 was the host, the first phage added dominated the infection; when B. subtilis strain SB11 was the host, beta22 produced progeny even when added to cells 5 min after infection with SP82. Dominance in these mixed infections could be correlated with qualitative and quantitative differences in the synthesis of phage-specific RNAs.


Induction of prophage SPO2 in Bacillus subtilis by 6-(para)-hydroxyphenylazouracil. DNA replication in Bacillus subtilis is reversibly inhibited by 6-(para)-hydroxyphenylazouracil (HpUra) (1971), whereas replication of temperature phage SPO2 is not affected by the drug. Experiments are presented which show that HpUra will induce bacteria lysogenic for SPO2. Also, prophage phi105, which is sensitive to the drug, is induced by HpUra. Induction of SPO2 lysogenics in the presence of HpUra gives selective synthesis of SPO2 DNA.


Origin and metabolic properties of the RNA species formed during the replication cycle of virus 2C. When short pulses of [(3)H]uracil were administered to Bacillus subtilis infected with phage 2C, the main species of labeled RNA was a 10S component that hybridized chiefly, but not exclusively, with the heavy strand of 2C DNA. After long pulses, most of the radioactivity was found in the 23S, 16S, and 5S rRNA's, which are coded for by the cell genome. Formation of such RNA species was reduced but not suppressed upon infection, the extent of inhibition being proportional to the virus-to-cell ratio. When bacteria were incubated with virginiamycin, an inhibitor of protein synthesis, and then infected with phage 2C, formation of virus-specific RNA decreased. This antibiotic also reduced the preferential transcription of the heavy strand of 2C DNA. The methylation pattern of rRNA remained unchanged upon infection with phage 2C. Virginiamycin reduced both the methylation and stability of rRNA in uninfected cells; this effect, however, was clearly reduced during the viral cycle. It can be concluded that in 2C-infected B. subtilis, cellular and viral RNA species are simultaneously synthesized and a preferential transcription of viral message depends not only on the number of available copies of viral template, but also on their translation. Moreover, virus-dictated proteins are responsible for the inhibition of cellular RNA formation as well as for the asymmetrical transcription of phage genome. Finally, virginiamycin and phage 2C have antagonistic, nonoverlapping effects on the metabolism and function of the RNA of the host cell.


Induction of prophage SPO2 in Bacillus subtilis: prophage excision in the absence of bacterial or bacteriophage DNA synthesis. Bacillus subtilis lysogenic for SPO2 wild type was induced under conditions preventing synthesis of both bacterial and phage DNA. The infectivity of phage DNA in transfection is strongly decreased under these conditions, whereas the activity of single phage genes as measured by marker rescue with superinfecting phage is unaffected. DNA from induced cells was sedimented in neutral sucrose gradients. After induction, phage DNA was detected at a position in the gradients, which was different from the bulk of the bacterial DNA, corresponding to linear double-stranded DNA of about 25 x 10(6) daltons. Similar results were obtained with bacteria lysogenic for a SPO2 prophage carrying a DNA-negative mutation. No separation of phage and bacterial DNA activity was detected when chloramphenicol was present during the induction period. These experiments show that prophage SPO2 can excise from the bacterial chromosome without previous replication.


In vivo and in vitro transcription of SPP1 DNA by host RNA polymerase. Most (if not all) SPP1 RNA can be synthesized in infected cells in the presence of chloramphenicol, or in vitro by Bacillus subtilis RNA polymerase.


A statistical method for comparison and classification of the microfilarial periodicity.


Pathogenic and antigenic properties of the Tyzzer's organisms from feline and hamster cases.


A comparative study of mycobacteria from patients' room dusts and from sputa of tuberculous patients. Source of pathogenic mycobacteria occurring in the sputa of tuberculous patients as casual isolates.


Preparation of an adjuvant-active, tuberculin-free peptidoglycolipid from human tubercle bacilli.


Further confirmation of the avirulence of Streptococcus faecalis strain BIO-4R.


Littré's femoral hernia causing intestinal fistula.


Further scanning electron microscope studies of the human glomerulus.


The ultrastructure of the skin in patients with mucopolysaccharidoses.


The content of some B-group vitamins in single-variety apple juices and commercial ciders.


Progesterone and oestrogen metabolism in the pregnant marmoset (Callithrix jacchus).


Detection of hepatitis-associated antigen. The latex agglutination test.


Gastrointestinal alterations in protein-calorie malnutrition.


Esophageal physiology.

Esophageal physiology.


Amino acid metabolism in parenteral nutrition: with special reference to the calorie: nitrogen ratio and the blood urea nitrogen level.


Medicine and millions.

Medicine and millions.


Letter: Psychosis associated with sympathomimetic and cromoglycate therapy.


Editorial: Famine in Ethiopia.

Editorial: Famine in Ethiopia.


Editorial: Management of maternal isoimmunization.


Total parenteral nutrition in paediatric patients.


The liquor amnii bilirubin-protein ratio in the management of Rhesus isoimmunization.


Tobramycin therapy in renal failure.

Tobramycin therapy in renal failure.


The specificity of transcription in vitro of the tryptophan operon of Escherichia coli. II. The effect of Rho factor.


Biosynthesis of RNA polymerase in Escherichia coli. I. Control of RNA polymerase content at various growth rates.


The analog inhibitor, alpha-methyl dopa, as a screening agent for mutants elevating levels of dopa decarboxylase activity in Drosophila melanogaster.


Bremsstrahlung radiators and beam filters for 25-MeV cancer therapy.


Transformation in Bacillus subtilis using excreted DNA.


A spectrophotometric study of the interaction of deoxyribonucleoproteins with the acriflavine-phosphotungstic acid complex.


Experimental determination of the need for multitaper collimators for neutron radiotherapy.


Macromolecular structure of the DNA of Bacillus bacteriophages.


Errors in DNA replication in bacteria in the presence of a thymidylic acid deficiency.


The Bacillus megaterium ribonucleotide reductase: evidence for a B 12 coenzyme requirement.


Production of L-phenylalanine by DL-phenylalanine hydroxamate-resistant Tyr- mutants of Bacillus subtilis.


Separation of two monoamine oxidases from bovine bran.


Isolation and characterization of a 5S RNA-protein complex from human placental ribosomes.


Investigation on radiosensitive and radioresistant populations of Drosophila melanogaster. VI. Selection for further increase in relative radioresistance of stage-7 oocytes of the irradiated population RO I.


Dose protraction and induced compound autosome non-disjunction in immature oocytes of Drosophila melanogaster.


The effects of radiation exposure-rate and exposure fractionation on the frequencies of recessive and dominant lethals in immature oocytes of Drosophila melanogaster.


A model to explain the response of stage 7 oocytes of Drosophila to the induction of dominant lethals under conditions of exposure-protraction and fractionation.


The development and evaluation of immunodiffusion, immunofluorescence and physiological tests for the differentiation of atypical Trichophyton rubrum and T. mentagrophytes isolates.


Failure of disodium etidronate in calcinosis due to dermatomyositis and scleroderma.


Letter: TRH and urinary urgency.

Letter: TRH and urinary urgency.


Letter: Children, diabetic ketoacidosis, 2,3-DPG, and growth potential.


Medicine and Public Affairs. From the tapes: Mr. Nixon on cholesterol, insomnia, etc.


Release of enzymes from cell walls by an endopectate-trans-eliminase.


New RNA polymerase from Bacillus subtilis infected with phage PBS2.


Sensitivity of cytotoxic T cells to T-cell mediated cytotoxicity.


In vitro activity of cells from genetically lethal embryos of Drosophila.


TRH: a possible mediator of thermoregulation.


Transfection of non-host bacterial spheroplasts with bacteriophage phi chi 174 DNA.


Decline of PCB concentrations in North Atlantic surface water.


Precocious development of detoxicating enzymes following pituitary graft.


Presence of J chain in human immunocytes containing various immunoglobulin classes.


Evidence for a direct effect of LRF and TRF on single unit activity in the rostral hypothalamus.


Bacterial mutation affecting T4 phage DNA synthesis and tail production.


Editorial: Intercellular junctions: sites of permeability barriers and cellular communication.


Neutrophil function in congenital phosphoglycerate kinase deficiency.


In vitro maturation of precursors of 5S ribosomal RNA from Bacillus subtilis.


Immunoglobulin of T lymphoma cells is an integral membrane protein.


Candidate for immunoglobulin D present on murine B lymphocytes.


Immunochemical analysis of IgD-lambda paraprotein in myeloma.


Orosomucoid and haptoglobin excretion in rat glomerulonephritis and tubulonephritis.


Long-term management of heart defects.

Long-term management of heart defects.


Diabetes mellitus in childhood.

Diabetes mellitus in childhood.


Breast-feeding: second thoughts.

Breast-feeding: second thoughts.


Rehabilitation of the long-stay schizophrenic.


Lupus nephritis: long-term follow-up.

Lupus nephritis: long-term follow-up.


Nursing care study: a losing battle?

Nursing care study: a losing battle?


Rheumatic diseases in the community.

Rheumatic diseases in the community.


Boredom in long-stay hospitals.

Boredom in long-stay hospitals.


The SEN in the psychiatric hospital.

The SEN in the psychiatric hospital.


Evidence for somatic generation of antibody diversity. RNA preparations containing 70-80% mouse kappa-chain mRNA have been prepared. The remainder consists of many RNA species, each of which represents a small fraction of the total RNA. The kappa-chain mRNA preparation hybridizes with mouse liver DNA with bi-phasic kinetics, indicating that it consists of two fractions -"unique" and "reiterated." Competition hybridization experiments show that the homology among the unique fractions from different mRNAs is the same as the homology among the amino acid sequences of the corresponding kappa-chains. Hence, in addition to the C-region (constant-region) sequences, (most of) the V-region (variable-region) sequences are also derived from unique germ line genes. The reiterated fractions from different kappa-chain mRNAs show essentially complete homology with each other. This fraction seems to consist mostly of sequences which do not code for amino-acid sequences of the secreted polypeptide chain, i.e., the "external" section of the mRNA molecule. It is concluded that the number of germ line genes is too small to account for the observed diversity of antibody molecules.


Antiinflammatory effects of murine malignant cells. Development of teratocarcinoma does not impair immunization of mice against Listeria monocytogenes. Endotoxin injection a short time before tumor cell inoculation allows the growth of teratocarcinoma in non syngenic mice despite immune stimulation. In contrast with this absence of impaired systematic immunity, teratocarcinoma cells were found to repulse macrophages in vitro. This effect on macrophages was also found with three other malignant cells and with trophoblast cells. In vivo, teratocarcinoma cells were found to impair local inflammation. These cells and other malignant cells are able to produce a compound(s) of molecular weight between 10(3) and 10(4), which prevents inflammatory reaction. These results suggest that mouse teratocarcinomas and other tumors by-pass the host immunological system of surveillance by at least two mechanisms: a direct toxic effect on macrophages and the release of an inhibitor of inflammation. The possible relations between these properties of malignant cells and physiological functions of trophoblast are discussed.


Mouse immunoglobulin heavy chains are coded by multiple germ line variable region genes. The N-terminal 20 residues of 13 heavy immunoglobulin chains from myeloma protein of the BALB/c mouse are compared with the same residues of 15 other heavy chains described in the literature. Sixteen of 28 sequences are different from one another. These proteins fall into four major sets, with 18 of the proteins in the largest set being further divisible into at least five subsets. This pattern of diversity suggests there are at least eight germ line genes coding for the variable regions of mouse heavy chain. Many of the immunoglobulins from which these heavy chains are derived exhibit binding activity for various haptens. The differing hapten specificities are closely correlated with distinct primary amino-acid sequences.


Deficiencies or excesses of metabolites interfering with differentiation. Auxotrophic mutants of Bacillus subtilis need much higher concentrations of the required adenine, nicotinic acid, riboflavin, thiamine, or tryptophan for optimal sporulation than for maximal growth. Acetate can partially replace thiamine, indicating the importance of the pyruvate dehydrogenase system for differentiation. A glycerol-requiring mutant can sporulate only if its cells contain a small concentration of L-alpha-glycerol phosphate during development. This can best be achieved by excess (>/=5 mM) of extracellular alpha-glycerol phosphate, which enters B. subtilis very slowly. The results show that both biosynthetic and catabolic enzymes are often needed to maintain the precise balance of metabolites required for differentiation. Mutants unable to catabolize fructose 6-phosphate, glucose 6-phosphate, or alpha-glycerol phosphate do not sporulate as long as these compounds accumulate inside the cells; their development is blocked before prespore septa have formed.


Highly purified colicin E3 contains immunity protein. Colicin E3, even when highly purified, still contains about one molar equivalent of a second protein, "E3 immunity protein." The two proteins are bound together in a complex that can be dissociated only under strongly denaturing conditions, such as electrophoresis in sodium dodecyl sulfate-polyacrylamide gles or by gel filtration in 6 M guanidine.hydrochloride. Colicin and immunity protein were separated by preparative electrophoresis in sodium dodecyl sulfate-polyacrylamide gels. The immunity protein prepared in this way was shown to be functionally and immunologically identical to immunity protein purified from colicinogenic cells by other methods. Colicin E3 that had been freed of immunity protein was much more active than complexed colicin in inhibiting protein synthesis in vitro.


The three-dimensional structure of the fab' fragment of a human myeloma immunoglobulin at 2.0-angstrom resolution. The structural analysis of the Fab' fragment of human myeloma immunoglobulin IgGl(lambda) New has been extended to a nominal resolution of 2.0 A. Each of the structural subunits corresponding to the variable and to the constant homology regions of the light and heavy chains contains two irregular beta-sheets which are roughly parallel to each other and surround a tightly packed interior of hydrophobic side chains. About 50-60% of the amino-acid residues are included in beta-pleated sheets. Sequence alignments between the homology regions of Fab' New obtained by comparison of their three-dimensional structures are given. Some of the sequence variations observed in light and heavy chains and the role of the regions of hypervariable sequence in defining the size and shape of the active site of different immunoglobulin molecules are discussed on the basis of the three-dimensional model of Fab' New.


Prostaglandin endoperoxides. Novel transformations of arachidonic acid in human platelets. Arachidonic acid incubated with human platelets was converted into three compounds, 12L-hydroxy-5,8,10,14-eicosatetraenoic acid, 12L-hydroxy-5,8,10-heptadecatrienoic acid, and the hemiacetal derivative of 8-(1-hydroxy-3-oxopropyl)-9,12L-dihydroxy-5,10-heptadecadienoic acid. The formation of the two latter compounds from arachidonic acid proceeded by pathways involving the enzyme, fatty acid cyclo-oxygenase, in the initial step and with the prostaglandin endoperoxide, PGG(2), as an intermediate. The first mentioned compound was formed from 12L-hydroperoxy-5,8,10,14-eicosatetraenoic acid, which in turn was formed from arachidonic acid by the action of a novel lipoxygenase. Aspirin and indomethacin inhibited the fatty acid cyclo-oxygenase but not the lipoxygenase, whereas 5,8,11,14-eicosatetraynoic acid inhibited both enzymes. The almost exclusive transformation of the endoperoxide structure into non-prostaglandin derivatives supports the hypothesis that the endoperoxides can participate directly and not by way of the classical prostaglandins in regulation of cell functions. The observed transformations of arachidonic acid in platelets also explain the aggregating effect of this acid.


The localization of transfer RNA5Lys genes in Drosophila melanogaster. Transfer RNA(5) (Lys) was isolated and purified from Drosophila melanogaster. It was iodinated in vitro with sodium [(125)I]iodide and hybridized in situ to the polytene salivary gland chromosomes from Drosophila. Autoradiographs exposed three to four weeks allowed the localization of this tRNA to the right arm of chromosome 2 between bands 48F and 49A.


Prostaglandin synthetase system--resolution into oxygenase and isomerase components. The microsomal fraction of bovine vesicular gland catalyzed the conversion of [1-(14)C]8,11,14-eicosatrienoic acid to prostaglandin E(1) in the presence of tryptophan, hemoglobin, and glutathione. The prostaglandin synthetase system was solubilized by treatment of the microsomal fraction with Tween 20 in the presence of ethylene glycol. DEAE-cellulose column chromatography separated the enzyme into two fractions (Fractions I and II), both of which were required for prostaglandin E(1) synthesis. When Fraction I alone was incubated with 8,11,14-eicosatrienoic acid, an unstable compound accumulated. This compound was converted to prostaglandin E(1) by the addition of Fraction II. On the basis of its R(F) values on thin-layer chromatography, its reduction to prostaglandin F(1alpha) with stannous chloride, and the decomposition to prostaglandins F(1alpha), E(1), and D(1) at room temperature, the unstable intermediate was tentatively identified to be the 9,11-endoperoxide derivative, referred to previously as prostaglandin R(1) (Nugteren et al. (1973) Biochim. Biophys. Acta 326, 448-461) or prostaglandin H(1) (Hamberg et al. (1974) Proc. Nat. Acad. Sci. USA 71, 345-349). Of the three cofactors mentioned above, Fraction I required both tryptophan and hemoglobin, while only glutathione was necessary for Fraction II. Anti-inflammatory agents such as indomethacin and aspirin were inhibitory to Fraction I.


Principles of fertilization.

Principles of fertilization.


Organization of immunoglobulin genes: reiteration frequency of the mouse kappa chain constant region gene. Hybridization kinetic analyses with synthetic DNA indicate that there are only two to three copies of the kappa constant region gene per haploid genome. This result lends weight to the argument that the immunoglobulin light chain is encoded by more than one continuous gene sequence.


Recent research on implantation in animals.


Effects of some amino acids on the content and uptake of histamine by neoplastic mast cells in vitro.


Enzyme derivatives containing reactive groups. Immobilization of alpha-amylase on human erythrocytes.


Delayed pressor response to isoproterenol during alpha-adrenergic blockade.


Effect of sex and castration on hepatic and intestinal activity of drug-metabolizing enzymes.


Proceedings: Behavioral effects of hypothalamic polypeptides in animals and man.


Long-term methylphenidate treatment of hyperkinetic children.


Long-term patients seen in an alcoholism clinic.


Letter: Insensitivity of serially transferred Tetrahymena pyriformis W. to irradiated media.


The roentgenographic manifestations of cutis laxa (generalized elastolysis).


The radiographic findings in congenital cutis laxa.


The effects of prostaglandins, prostaglandin inhibitors, and oxygen on the closure of the ductus arteriosus, pulmonary arteries and umbilical vessels in vitro.


Theileria parva: comparative infectivity of a ground tick stabilate and a classical 10-tick challenge.


Comparative development and evolution of the placenta in primates.


Evolutionary trends in primate sex cycles.


Comparative morphology of the primate ovary.


Infections in cancer patients. I. Bacterial infections.


Placental biosynthesis and metabolism of steroid hormones in primates.


Biology of primate spermatozoa.

Biology of primate spermatozoa.


Nursing challenges: treatment and care of the patient with hyperparathyroidism.


A systematic study of variables affecting protein binding of thyroxine and triiodothyronine in serum.


The hydrolysis of pteroylpolyglutamates in the small intestine.


Influence of feeding and sham feeding upon serum gastrin and gastric acid secretion in control subjects and duodenal ulcer patients.


Comparison of methods to diagnose deficiency or malabsorption of vitamin B12.


Serum B12 determination using the radiosorbent technique and the Euglena gracilis assay.


The absorption of vitamin B12 in the blind-loop syndrome.


IgM turnover in chronic liver disease.

IgM turnover in chronic liver disease.


Pathophysiology and current treatment of intestinal obstruction.


Notions of morality in medical organization and practice.


Mutagens and potential mutagens in the biosphere. I. DDT and its metabolites, polychlorinated biphenyls, chlorodioxins, polycyclic aromatic hydrocarbons, haloethers.


Autoimmune haemolytic anaemia: serological and immunochemical characteristics of the autoantibodies; mechanisms of cell destruction.


Studies of monkeys asphyxiated at birth: implications for minimal cerebral dysfunction.


Autoimmune haemolytic disease in NZB mice.


Proliferation and maturation of bone marrow-derived lymphocytes.


The identification of mouse myeloma cells which have undergone mutations in immunoglobulin production.


Cromolyn sodium therapy in childhood asthma.


Current diagnosis and treatment of acute pancreatitis.


Delayed recognition of lead encephalopathy in two "moonshine" drinkers.


The Indonesian developmental area study: observations on mammals from South and East Kalimantan (Borneo).


Pulmonary tuberculosis in a rural area of Sarawak, Malaysia.


Sarcocystis from rats in Sulawesi, Indonesia.


Observations on exflagellation of microgametocytes of Leucocytozoon sabrazesi.


Leucocytozoon sabrazesi-first record from the Malayan jungle fowl, Gallus gallus spadiceus.


Further stages of the "sporozoan" from the moon rat Echinosorex gymnurus, and its possible connection with Sarcocystis.


Cytological analysis of the variability of ring chromosomes in Drosophila. Communication II. Influence of temperature, the Gowen gene, and heterozygous inversion.


The silvered leaf monkey as a natural host of the lung mite, Pneumonyssus simicola Banks, (Acarina: Halarachnidae) in Malaysia: a preliminary report.


A study of the homeotic genes antennopedix and nasobemia in Drosophila melanogaster. Communication II. Interaction of the homeotic antennopedix and nasobemia genes with the polycomb, multiple sex comb, aristapedia, and thread genes.


Investigation of the genetics of the sexual behavior of Drosophila melanogaster. Communication III. Further genetic analysis of the lines differeing in sexual activity of the males.


Development of mesosomes in Caryophanon.


The mutagenic effect of nitrosoalkylamides on extracellular phages and bacterial transforming DNA.


Letter: Intra-epithelial lymphocytes in the jejunum of malnourished children.


Sterilisation of laryngoscope blades. A preliminary report.


A comparison of oral feeding and total parenteral nutrition in infants of very low birthweight.


Intravenous fructose disappearance in protein calorie malnutrition.


Health care and justice.

Health care and justice.


Blood xylose concentrations in protein energy malnutrition. Relationship to serum albumin and jejunal histology.


Fungi associated with common crops and crop products and their significance.


Hypercholesterolaemia and atherosclerosis induced in vervet monkeys by cholesterol-free, semisynthetic diets.


Controversies in the management of potentially curable breast cancer.


Topical burn wound therapy.

Topical burn wound therapy.


Three varieties of congenital diverticulum of the intestine.


Interdisciplinary approach to advanced ovarian cancer.


The relationship of genitourinary tract procedures and deep sepsis after total hip replacements.


Evaluating long-term periodontal therapy.


Effect of physical activity before pregnancy on fetuses of mice exercised forcibly during pregnancy.


Pseudomonas aeruginosa abscess masquerading as a slowly growing solitary pulmonary nodule.


Maltose metabolism in diabetic state.

Maltose metabolism in diabetic state.


Immunological studies in patients receiving repeated allogeneic anc xenogeneic liver hemoperfusions.


Lesions of bladder muscle in baboons and monkeys infected with schistosoma haematobium.


Symposium on renal lithiasis. Nutritional support of the azotemic patient.


Hematuria in children. A guide in differential diagnosis.


Intraprostatic injections of various antitumoral compounds into rats.


Human hepatitis B antibody: evaluation of assays and development of a reference panel.


Infection stones of prostate gland. Laboratory diagnosis and clinical management.


Renal function after thyroidectomy and parathyroid hormone administration. An experimental study in the pig.


Syndrome of dyspraxia and multifocal seizures associated with chronic hemodialysis.


Long-term membrane oxygenation without systemic heparinization.


Preliminary studies with the fistula catheter - a new vascular access prosthesis.


A method for serial measurement of regional cortical metabolism and blood flow.


Coated adsorbents for direct blood perfusion II.


Fundamental biophysical aspects of high-energy radiations in cancer therapy.


A new technique employing extracorporeal chemotherapy.


An unidentified sporozoan encephalomyelitis in sheep.


Spontaneous malignant lymphoma in a new-world primate.


Ultrastructural studies of spleens, brains, and brain cell cultures of mice with scrapie.


Toxoplasma-like encephalomyelitis in the horse.


Quantitative studies on serum immunoglobulin levels in suckled calves from birth to five weeks.


Brucellosis in wildlife.

Brucellosis in wildlife.


Brucella abortus biotype 3 in Tanzania.

Brucella abortus biotype 3 in Tanzania.


Serological relationship between maize rough dwarf virus and rice black-streaked dwarf virus.


In vitro interaction between phage and receptor lipopolysaccharide: a novel glycosidase associated with Salmonella phage epsilon15.


Low-frequency specialized transduction with Bacillus subtilis bacteriophage phi 105.


Detection of mycoviruses using antiserum specific for dsRNA.


Bacteriophage gene expression in sporulating cells of Bacillus subtilis 168.


Isolation and genetic mapping of temperature-sensitive mutants of cyanophage LPP2-SPI.


Spectral sensitivity of the short wavelength mechanism in the squirrel monkey visual system.


Inhibition of toad (Bufo bufo L) retinal on-off and off-ganglion cells via active eye closing.


Old age--a problem for society as a whole.


Total parenteral alimentation.

Total parenteral alimentation.


Gonorrhea from birth through adolescence: local and systemic manifestations.


The conjugation of indolylacetic acid in man, monkeys and other species.


Rapid differentiation of certain bacteria in mixed populations by gas-liquid chromatography.


Studies on the methodical and biological variation of proteins quantitated by single radial immunodiffusion.


Interaction of nutrition and infection: Trypanosoma lewisi, folic acid levels in sera and tissues of normal and folic acid-deficient rats.


The isoenzymes of lactate dehydrogenase of bovine thrombocytes.


Developmental biochemistry of bovine foetal muscle. II. Collagen.


Studies on the effect of temperature and glycerol on the inactivation of the virus of haemorrhagic septicaemia (VHS) of rainbow trout by use of the microtiter system.


Control of Pseudomonas aeruginosa infections.


Immunoglobulins G, A, and M in bronchial asthma.


Bronchial reactivity to inhaled histamine in asthmatic patients, before and after administration of atropine, phentolamine or disodium cromoglycate.


New developments in asthma therapy.

New developments in asthma therapy.


Selection of patients for cromolyn therapy.


Environmental factors and host responses in asthma.


Fine structural localization of alkaline phosphatase activity of the female genital canal surface during the estrous cycle and after castration in rats.


Comparative nitrogen balance studies with an amino-acid solution based on nutritional studies against two protein-based solutions.


Cystine: a semi-essential amino acid in the newborn infant.


Resting metabolic rate in malnourished babies in relation to total body potassium.


Onset of diabetic ketoacidosis with symptoms of acute abdomen during oral mestranol treatment.


The isolation of Mycoplasma primatum during an autopsy study of the mycoplasma flora of the human urinary tract.


Pseudomonas aeruginosa infection in cystic fibrosis. Occurrence of precipitating antibodies against pseudomonas aeruginosa in relation to the concentration of sixteen serum proteins and the clinical and radiographical status of the lungs.


Mutations in Arthrobacter affecting formation of extracellular protease.


Infection of Arvicola terrestris (vole rat) with M. tuberculosis and M. bovis.


Antimycobacterial antibodies in sera from patients throughout the clinico-pathological disease spectrum of leprosy.


Determination of antistreptolysin O by reversed single radial immunodiffusion.


DDT and related substances: effects on permeability properties of myelinated xenopus nerve fibre. Potential clamp analysis.


Study of lysogenic Bacillus cereus strains by electron microscopy.


(14C)adenine uptake into Bacillus anthracis adenylsuccinate lyase deficient mutant.


A highly specific phage attacking Bacillus anthracis strain Sterne.


Bacteriological and pharmacological investigations with Garamycin (gentamicin).


Study of auxotroph mutants induced by copper sulphate in Bacillus subtilis.


Bacterial contamination and sparing heat treatment of mother's milk.


Topologic analysis of the fate of transfecting SP50 DNA in competent cells of Bacillus subtilis.


Pirazocillin: a new acid and penicillinase resistant semisynthetic penicillin.


Physicochemical studies of Ca++ controlled antigen antibody systems.


13C NMR studies of the interaction of Hb and carbonic anhydrase with 13Co2.


The application of perturbed directional correlation of gamma rays to the study of protein-metal interactions.


The functional roles of metals in metalloenzymes.


Nutrition and malnutrition. Identification and measurement. Definition of the problem.


Anthropometry as an index of nutritional status.


Relative values of longitudinal, cross-sectional and mixed data collection in community studies.


From a QUAC stick to a compositional assessment of man's nutritional status.


Ecological factors relating to child growth and nutritional status.


Behavioral effects of artificial gravity: equivalence of rotation rate and radius in controlling gravity-avoidance behavior.


Coprophagy in laboratory animals: a preliminary study to assess potential influences of excreta ingestion on radionuclide retention patterns.


Letter: Heat stability of serum pyridoxal.


Special problems associated with the isolation and identification of intestinal bacteria in fecal flora studies.


A comparison of methods for estimating human body composition.


A new method for determination of alpha-1-antitrypsin phenotypes using isoelectric focusing on polyacrylamide gel slabs.


Demonstration of IgE on human-skin mast cells using anti-IgE125I and refined light microscopy.


Effect of incubation time on Lactobacillus casei bioassay. Variation with two different commercial assay media.


Parenteral hyperalimentation--Five years' clinical experience.


Letter: Trace element requirements in hyperalimentation.


Letter: Stability of phytonadione in hyperalimentation fluids.


Correlation of low serum IgM levels with absence of functional splenic tissue in sickle cell disease syndromes.


Discoid lupus erythematosus with macroglobulinemia. Correlations between autoimmunity and B cell malignancy with hyperimmunoglobulinemia.


Arthritis in acute hepatitis and chronic active hepatitis. Pathology of the synovial membrane with evidence for the presence of Australia antigen in synovial membranes.


Familial myeloma. Report of eight families and a study of serum proteins in their relatives.


Epidemic viral hepatitis, type B, in hospital personnel.


Auto-immune hemolytic anemia.

Auto-immune hemolytic anemia.


Crisis intervention for ghetto children: contraindications and alternative considerations.


Time course of isotonic contraction in single cells and muscle strips from Bufo marinus stomach.


Absence of edge damage in toad urinary bladder.


Effects of stimulus conditions, temperature, and length on energy output of frog and toad sartorius.


Letter: Dominance, sexual preference, and sexism.


Influence of fractionation on acute and late reactions in vocal cord carcinoma. Four schedules for the treatment of localized vocal cord carcinoma were compared, to establish a relationship between daily and total dose, and acute and late normal tissue reactions. All patients were treated 5 days per week. All of those who received 200 R daily (6,000 R in 30 treatments) developed a confluent mucositis and minimal to moderate subsequent aryepiglottic edema. Patients who received 180 R daily (6,480 R in 36 treatments) developed a patchy mucositis. Those who received 170 R daily (7,140 R or 6,400 rads in 42 treatments) developed only a brisk erythema, with decreasing incidence and severity of edema. At 160 R daily (6,880 R in 43 treatments) only a mild erythema developed, with no visible edema at any time. Local recurrence rates of about 10 percent for T1 lesions, and of about 25 per cent for T2 and T3 lesions, indicate that the decrease in reactions with longer fractionation has been achieved without loss of tumor control. Even minimal improvement in functional status following treatment for this highly curable malignancy well justifies the inconvenience of the increased number of treatments. The present formula of Ellis for normal tissue tolerance is not consistent with these findings, since the NSDs are essentially identical for all 4 schedules, while tolerance is notably improved by increasing fractionation.


Kaposi's sarcoma. Experience with ten cases. Kaposi's sarcoma is uncommon in the U.S.A. and common in Africa. Men are affected at least 4 times more frequently than females. It occurs simultaneously with lymphomas and resembles them in several ways. Radiation therapy controls the disease well. Ten patients were seen with the disease. Most had a relatively benign course, especially the 4 women. Treatment with supervoltage irradiation and cryosurgery was effective in controlling local disease.


Computer generated isodose curves for high energy x-ray machines. Experimental verification and time-saving strategies. Calculation of isodose patterns for high energy therapy units using Cunningham's BEAM program requires accurate information on the beam profile in air. This may be obtained by scanning across the x-ray beam using a very small silicon diode detector. Data obtained on a particular unit may not be applicable to any other unit. Computer calculations of isodose curves for high energy x-ray machines must use a 0.25 cm. or smaller lateral grid of dose calculation points to ensure accurate reproduction of the sharp fall-off of the dose at the beam edge. Computer calculation time to obtain the isodose curves using this size lateral grid is prohibitively long. The computing time can be appreciably reduced by: (1) changing the width of the annular sectors used in calculating the contribution of scattered radiation to the dose to 0.5 cm.; and (2) calculating the dose at 0.25 cm. lateral intervals within 1 cm. of the beam edge, but changing the spacing of the grid to 1 cm. inside the field. These programming strategies do not produce any significant change in the isodose curves but lead to a significant reduction in computer time.


Design of X-ray targets for high energy linear accelerators in radiotherapy.


Schistosoma mansoni circulating antigen: detection by complement fixation in sera from infected hamsters and mice.


Angiographic features of arterial lesions in neurofibromatosis.


Late assessment of results of cricopharyngeal myotomy for cervical dysphagia.


Pyrexia following open heart surgery. The role of bacterial infection.


Use of tobramycin in eradicating experimental bacterial endophthalmitis.


Removal of proteases from DNase I by chromatography over agarose with covalently attached lima bean protease inhibitor.


The determination of chlorine in organic materials by combustion and microcoulometry.


Anomalous behaviour of EDTA during gel filtration. Studies on the possible contamination of the S100 protein.


Synthesis of the active derivative of bovine trypsin-kallikrein inhibitor (Kunitz) with open peptide bond in the reactive site.


Proteinase catalyzed resynthesis of the peptide bond Lys 15--Ala 16 in the derivative of trypsin-kallikrein inhibitor (Kunitz) with this peptide bond hydrolyzed.


Replacement of lysine by arginine, phenylalanine, and tryptophan in the reactive site of the trypsin-kallikrein inhibitor (Kunitz).


Inactivation of the trypsin-kallikrein inhibitor (Kunitz) by cleavage of the dipeptide Ala 16--Arg 17 in the reactive site.


Evaluation of agar gel immunodiffusion for detection of immunologic response of pigs to experimental infection with hemagglutinating encephalomyelitis virus.


Intestinal absorption of D-xylose and folic acid measured by a perfusion technique in patas monkeys (Erythrocebus patas).


Epidemiologic studies relating thermophilic fungi and hypersensitivity lung syndromes.


The purification of mycobacterial polysaccharides with concanavalin A.


Letter: The impact of chemotherapy on the care of patients with tuberculosis.


Active and passive immunization in the treatment of experimental burn wound sepsis from Pseudomonas.


Group-y meningococcal disease. Twelve cases at an army training center.


Treatment of diphtheria carriers: benzathine penicillin, erythromycin, and clindamycin.


Letter: Variant anginal syndrome, coronary artery spasm, and ventricular fibrillation in absence of chest pain.


Comparative structure of myoglobin: primates and tree-shrew.


Rodent hemoglobin structure: a comparison of several species of mice.


Multiple hemoglobin alpha-chain loci in monkeys, apes, and man.


Exploratory studies on detection of hemoglobins in the early human fetus in utero.


Effects of membrane potential on sodium and potassium fluxes in squid axons.


A delayed side effect of topical thiotepa.


Space microbiology.

Space microbiology.


Persisting bacteriophage infections, lysogeny, and phage conversions.


Viroids: the smallest known agents of infectious disease.


Virus-induced modification of cellular membranes related to viral structure.


Mechanisms of inorganic oxidation and energy coupling.


An immunological and electrophoretic study of Rhodospirillum rubrum chromatophore fragments.


Proceedings: Parenteral feeding: metabolic changes.


Calorimetric measurement of the enthalpy of magnesium binding to yeast enolase.


Clinical diagnosis of the monoclonal gammopathies.


Proceedings: Parenteral feeding: surgical indications and techniques.


Proceedings: Use of diphosphonates in diseases associated with ectopic calcification.


Polychlorinated biphenyls. Storage, distribution, excretion, and recovery: liver morphology after prolonged dietary ingestion.


A reference blood as a control of parameters in Coulter S.


Atherosclerotic coronary artery aneurysm. Report of five cases and review of literature.


Disseminated toxoplasmosis in the compromised host. A report of five cases.


Prolonged trance-like stupor in epilepsy. Petit mal status-wave stupor, spaced-out status.


Gonococcal lymphadenitis.

Gonococcal lymphadenitis.


One-step isolation of microbial ribulose-1,5-diphosphate carboxylase.


Estimation of the genome size of blue-green algae from DNA renaturation rates.


Studies on gliding motility in Myxococcus xanthus.


Immunochemical determination of human pregnancy proteins.


Distribution of gastrin in human digestive organs demonstrated by direct immunofluorescence.


Nursing care of the patient being maintained on long-term peritoneal dialysis.


Retina and optic nerve after posterior ciliary artery occlusion. An experimental study in squirrel monkeys.


Effect of lidocaine on the antibacterial activity of seven antibiotics.


Ocular manifestations of Conradi disease.


TheThe genetic architecture of resistance to desiccation in populations of Drosophila melanogaster and D. simulans.


Drinks, drugs - the doctor's dilemma.

Drinks, drugs - the doctor's dilemma.


A new look at an age old problem.

A new look at an age old problem.


Preprogrammed and feedback-guided movements of monkeys.


Genetic control of activity, preening, and the response to a shadow stimulus in Drosophila melanogaster.


Recommended methods used in the nutritional status assessment: clinical signs and symptoms.


Reasons for, and methods in dietary and food consumption surveys.


Genetics of esterases in Drosophila. III. Influence of different chromosomes on esterase pattern in Drosophila.


Maroteaux-Lamy disease (mucopolysaccharidosis VI), subtype A: deficiency of a N-acetylgalactosamine-4-sulfatase.


Rabbit beta-glucuronidase. Subcellular distribution and immunochemical properties. 1. The subcellular distribution of beta-glucuronidase and other hydrolases in rabbit liver was investigated. beta-Glucuronidase was found in both microsomal and lysosomal fractions. 2. Multiple forms of beta-glucuronidase were present in extracts of microsomal and lysosomal fractions. All forms were common to both fractions. 3. A specific antiserum against beta-glucuronidase was raised, and characterized by immunoprecipitation and affinity-chromatography procedures. 4. The immunological identity of the multiple forms in the pure beta-glucuronidase preparation, and the immunological identity of the beta-glucuronidase complement of lysosomal extracts with that of microsomal extracts, were demonstrated by means of the antiserum. The presence of inactive enzyme in various enzyme preparations was shown.


Rabbit beta-glucuronidase. Purification and properties, and the existence of multiple forms. 1. beta-Glucuronidase (EC 3.2.1.31) was purified from rabbit liver by a procedure involving autolysis, (NH(4))(2)SO(4) fractionation, chromatography on DEAE-cellulose and hydroxyapatite, gel filtration, sedimentation in a sucrose gradient, and isoelectric focusing. 2. Electron microscopy revealed ferritin as the major contaminant in later stages of purification and also showed aggregates of enzyme molecules. Particular attention was paid to the removal of ferritin. 3. The purified enzyme was homogeneous in polyacrylamide-gel electrophoresis both in non-dissociating conditions and in the presence of sodium dodecyl sulphate, and in Ouchterlony gel diffusion and immunoelectrophoresis against polyspecific antisera. 4. Sedimentation in sucrose gradients gave a molecular weight of 300000, whereas gel filtration indicated 440000. 5. Subunits of 75000 molecular weight were observed in gel electrophoresis in the presence of sodium dodecyl sulphate and in gel filtration in the presence of urea. 6. The K(m) value for p-nitrophenyl beta-d-glucuronide was 0.6mm, and the enzyme was extremely sensitive to lactone inhibitors. It was also inhibited by Hg(2+) ions. 7. Multiple forms were observed in the pure enzyme by isoelectric focusing, with pI values of 4.5-5.8. Subunits showed similar heterogeneity. The origin of the multiple forms was investigated in detail, and the possibility of artifact generation largely excluded. Some of the forms of lowest pI disappeared after neuraminidase digestion. The nature of the residual heterogeneity remains to be elucidated.


Change of enzyme activities during the early stage of influenza virus infection.


Avian infectious bronchitis: demonstration of serum IgG- and IgM-neutralizing antibody by sucrose density-gradient centrifugation and mercaptoethanol reduction.


Virucidal activity of commercial disinfectants against velogenic viscerotropic Newcastle disease virus.


Chelation of divalent cations by lomofungin: role in inhibition of nucleic acid synthesis.


Structure-activity relationships in the effects of 1-alkylimidazoles on microsomal oxidation in vitro and in vivo.


Monoamine oxidase inhibitors and other drugs as inhibitors of diamine oxidase from human placenta and pig kidney.


7-nitro-8-acyloxyquinolines with antimicrobial and antifungal activity.


Clinical features and serologic abnormalities in juvenile rheumatoid arthritis.


Antimicrobial studies in vitro with cephacetrile, a new cephalosporin derivative.


Letter: Serum gold determinations.

Letter: Serum gold determinations.


Pharmacokinetic studies in animals and humans of a new cephalosporin, the sodium salt of 7-cyanacetyl-amino-cephalosporanic acid.


Letter: Long-term side effects of azathioprine.


Experimental pseudomonas osteomyelitis: treatment with sisomicin and carbenicillin.


Proceedings: Postoperative measurement of blood flow in saphenous vein bypass grafts.


Qualitative characteristics of anti-DNA antibodies in lupus nephritis.


Immunoglobulin E (IgE) levels in serum and synovial fluid in rheumatoid arthritis.


Investigation of mechanisms of action of anti-tumour factors made from lymph nodes or spleens of allergized allogeneic mice.


Intracellular killing of different strains of Pseudomonas aeruginosa by human leucocytes.


Vaccine potencies of the live vaccine strain of Francisella tularensis and isolated bacterial components.


Hepatocellular uptake of ferritin in the rat.


Plant carbonic anhydrase. Hydrase activity and its reversible inhibition.


Studies on interaction between poly(L-lysine 40, L-alanine 60) and deoxyribonucleic acids.


Synthesis of oligodeoxyribonucleotide ethyl phosphotriesters and their specific complex formation with transfer ribonucleic acid.


The identification of poly-gamma-glutamyl chain lengths in bacterial folates.


Factors involved in the synthesis of cyclopropane fatty acids by Streptococcus faecalis.


Effect of diet on hepatic and intestinal lipogenesis in squirrel, cebus, and cynomolgus monkeys.


Physical properties and biological activities of two forms of alpha-N-acetylglucosaminidase from bovine spleen.


Physiological differences between cyclopropane fatty acid-deficient mutants and the parent strain of Streptococcus faecalis.


Further characterization of human thyroxine-binding globulin.


Legume root nodule nitrogenase. Purification, properties, and studies on its genetic control.


Activation of platelet fibrin-stabilizing factor by thrombin.


Amino acid sequence of sheep carbonic anhydrase C.


SomeSome characteristics of a phospholipase A2 from sheep red cell membranes.


Lecithinase activities at the external surface of ruminant erythrocyte membranes.


Genetic complexity of mitochondrial DNA from Euglena gracilis.


Properties of mitochondrial DNA from Kluyveromyces lactis.


The attachment of polyribosomes to membranes of the hypocotyl of Phaseolus vulgaris.


Solubilization and properties of poly(ADP-ribose) polymerases from bovine spleen and Ehrlich ascites cells.


Size and configuration of mitochondrial DNA in Euglena gracilis.


Subunit structure of DNA-dependent RNA polymerase of Anacystis nidulans.


Loss of cellular material from suspensions of isolated epithelial cells of guinea pig small intestine.


Anti-h absorbed by, and eluted from Oh (Bombay) red blood cells.


Two proposed general configurations for bacterial cell wall peptidoglycans shown by space-filling molecular models.


Effect of metal binding on the intrinsic fluorescence of bovine carbonic anhydrase.


Qualitative and quantitative mass spectrometry of some noncatecholic biogenic amines and related compounds as their dansyl derivatives.


Treatment related observations in solid tissues, fibroblast cultured and amniotic fluid cells of type II glycogenosis, Hurler disease and metachromatic leukodystrophy.


Enzyme replacement in genetic disease. Prospectus.


Mucopolysaccharide-storage diseases: corrective activity of normal human serum and lymphocyte extracts.


Fabry disease: kidney transplantation as an enzyme replacement technic.


Fabry disease: correction of the enzymatic deficiency by renal transplantation.


Enzyme replacement in Fabry disease: treatment of cultured skin fibroblasts with a purified alpha-galactosidase from ficin.


Distribution of polychlorinated biphenyls in an aerated biological oxidation wastewater treatment system.


Polychlorinated biopenyls. V. Synthesis of 14C-labelled 2,2', 4,4',5,5'-hexachlorobiphenyl, a major component in PCB-mixtures.


Effects of dieldrin and PCB's upon the production and morphology of Japanese quail eggs.


Analyses of polychlorinated biphenyls in bird tissues and Aroclor standards with gas chromatography and mass spectrometry.


Recovery of trichlorfon (Dylox) and carbaryl (Sevin) in songbirds following spraying of forest for gypsy moth.


Interference in the electron-capture technique for determination of polychlorinated biphenyls by sulfur-containing compounds in petroleum products.


DDT, DDE and PCBs in the tissues of reef dwelling groupers (Serranidae) in the Gulf of Mexico and the Grand Bahamas.


PCB'S and p,p' DDE in the blood of cachectic patients.


Comparison of polychlorinated biphenyls in yusho patients and ordinary persons.


Time-course of induction of microsomal enzymes following treatment with polychlorinated biphenyl.


Dissipation of Guthion Sevin, Polyram, Phygon and Systox from apple orchard soil.


Bacterial degradation of polychlorinated biphenyls. I. Identification of some metabolic products from Aroclor 1242.


Accumulation of Aroclor 1254 in grass shrimp (Palaemonetes pugio) in laboratory and field exposures.


Gas chromatography and mass spectrometry of polychlorinated terphenyls (Aroclor 5460).


Effect of aflatoxin B1, ochratoxin and rubratoxin B on a protozoan, Tetrahymena pyriformis HSM.


DDE, PCB and aldrin levels in Arctic birds of Greenland.


Polychlorinated biphenyls and p,p' DDE in green turtle eggs from Ascension Island, South Atlantic Ocean.


A gas chromatographic-mass spectrometric comparison of polychlorinated biphenyl residues in the Japanese quail brain to an Aroclor standard.


High-volume collection of atmospheric polychlorinated biphenyls.


Embryotoxic and teratogenic effects in unhatched fertile eggs from hens fed polychlorinated biphenyls (PCBs).


Effects of nonpersistent pesticides on liver weight, lipids and vitamin A of rats and quail.


Chlorinated insecticide residues in the eggs of some freshwater fish.


Uptake of a PCB (Aroclor 1254) from soil by carrots under field conditions.


Effect of diethylstilbestrol on feedlot associated bacteria.


Gas-liquid chromatography--mass spectrometric characterization of aroclor 1242 and 1254 components.


Translocation of four organochlorine compounds by red mangrove (Rhizophora mangle L.) seedlings.


The effect of age and sex on the toxicity of Aroclor 1254, a polychlorinated biphenyl, in the rat.


Mercury and selected pesticide levels in fish and wildlife of Utah: II. Levels of mercury, DDT, DDE, dieldrin and PCB in chukars, pheasants and waterfowl.


Acute effects of Aroclor 1254 (PCB) on Ictalurus punctatus (catfish).


Avoidance of Aroclor 1254 by shrimp and fishes.


Mercury and selected pesticide levels in fish and wildlife of Utah: I. Levels of mercury, DDT, DDE, dieldrin and PCB in fish.


Presence of PCB, DDE and DDT in human milk in the provinces of New Brunswick and NOVA Scotia, Canada.


Efficacy of home laundering in removal of DDT, methyl parathion and toxaphene residues from contaminated fabrics.


The finding of chlorinated dibenzofurans in a Japanese polychlorinated biphenyl sample.


Some aspects of the recovery of chlorinated residues (DDT-type compounds and PCB) from fish tissue by using different extraction methods.


Effect of PCB ingestion on sleeping times, organ weights, food consumption, serum corticosterone and survival of albino mice.


Uptake of chlorinated paraffins and PCB from suspended solids and food by juvenile Atlantic salmon.


Polychlorinated biphenyl emission from fluorescent lamp ballasts.


Reduction of pentobarbital-induced sleeping times in PCB-treated cottontail rabbits.


Metabolism of 2,2'-dichlorobiphenyl-14C in two plant-water-soil-systems.


Residues of organochlorine insecticides and PCBs in samples of Norwegian butter 1966-1972.


The use of polyurethane foam plugs for extraction of polychlorinated biphenyls (PCB's) from natural waters.


The teeth of hominids, hominoids and pongids.


Jacksonian seizure arising from the contralateral hippocampus.


The relative effectiveness of super- and orthovoltage x rays for intestinal damage in man and mouse: an apparent discrepancy and its explanation.


Relative biological effectiveness (RBE) of 30 MeV electrons at varying depths in tissue.


Letter: SI Units in radiology.

Letter: SI Units in radiology.


Comparative radiobiological measurements with two high-energy cyclotron-produced neutron beams presently used for radiotherapy.


Letter: Recent advances in the development of a 14 MeV neutron generator suitable for radiotherapy.


Total body potassium in long-term frusemide therapy: is potassium supplementation necessary? Measurements of total body potassium (T.B.K.) were made by whole-body counting in four groups of patients receiving oral frusemide for one year. Patients in group 1 had essential hypertension and normal renal function and received 40 mg frusemide daily without potassium supplements. Patients in group 2 were similar but received oral potassium supplements for the first four months of treatment. Patients in group 3 had hypertension associated with renal disease and received 120 mg frusemide daily without potassium supplements. Patients in group 4 also had hypertension and renal impairment and in addition to 120 mg frusemide daily they received oral potassium supplements for four months. No evidence of depletion of T.B.K. was found in any of the groups after continuous treatment with frusemide for one year. It is questioned whether potassium supplementation in long term diuretic therapy with frusemide is necessary unless there is evidence of pre-existing potassium depletion or of some other factor such as cardiac failure, cirrhosis of the liver, or the nephrotic syndrome.


Letter: Two-phase blood culture system.

Letter: Two-phase blood culture system.


Letter: Gianotti-Crosti syndrome and viral infection.


Absorption of slow-release iron and effects of ascorbic acid in normal subjects and after partial gastrectomy. Radiolabelled tablets of slow-release iron and ferrous sulphate containing 50 mg of elemental iron were given to 28 patients, and iron absorption was studied using a whole body counter.There was no significant difference between the absorption of ferrous sulphate and that of slow-release iron in normal subjects, anaemic patients, anaemic and non-anaemic post-gastrectomy patients, or those with coeliac disease. Ascorbic acid potentiated iron absorption, especially in anaemic post-gastrectomy patients.The indications and hazards of slow release iron preparations are discussed.


Editorial: Medical nemesis.

Editorial: Medical nemesis.


Medical Nemesis--three views. "Medicalization" of health.


Medical nemesis--three views. Indictment of medical care.


Cerebral concussion and traumatic unconsciousness. Correlation of experimental and clinical observations of blunt head injuries.


A crescent-shaped cortical visual area surrounding the middle temporal area (MT) in the owl monkey (Aotus trivirgatus).


Choline acetyltransferase localization in the central nervous system by immunohistochemistry.


On the elicitability of vocalization from the cortical larynx area.


Catecholamine-binding protein in mouse brain: isolation and characterization.


Directed mutation in Streptomyces lipmanii.


Action of streptomycin on the growth of Streptomyces griseus.


Purification of Rickettsia rickettsi by density-gradient zonal centrifugation.


Minicell formation in chloramphenicol-treated Bacillus cereus.


Growth abnormalities in microorganisms exposed to tobacco leaf extracts.


Localization of neuroendocrine functions within the hypothalamus.


Myasthenia gravis associated with diphenylhydantoin therapy for epilepsy.


Letter: Rh sensitization following abortion.


Role of the ventricular system in neuroendocrine processes: synthesis and distribution of thyrotropin releasing factor (TRF) in the hypothalamus and third ventricle.


The effect of dexamethasone on TSH and prolactin secretion after TRH stimulation. Serum thyrotropin (TSH) and prolactin levels were measured after intravenous administration of 400 mug of synthetic thyrotropin-releasing hormone (TRH) in 13 normal subjects and six hypothyroid patients before and after three days of administration of dexamethasone 2 mg per day. In the normal subjects dexamethasone suppressed baseline serum levels and secretion of TSH after TRH stimulation. On the other hand, it had no effect on the hypothyroid patients. In the control group dexamethasone also suppressed baseline serum levels but not secretion of prolactin after TRH stimulation. Dexamethasone had no effect on prolactin levels in the hypothyroid group. It is concluded that in normal patients short-term administration of dexamethasone has an inhibitory effect on TSH secretion at the pituitary level. As for prolactin, our results could indicate that TRH is a more potent stimulator of prolactin secretion than of TSH secretion, or that TSH and prolactin pituitary thresholds for TRH are different.


Comparison of amoxicillin and ampicillin in single-dose oral treatment of males with gonococcal urethritis. Amoxicillin in single oral doses of 2.0 g, 2.0 g plus 1.0 g probenecid, or 3.0 g was compared with ampicillin 3.5 g plus 1.0 g probenecid in the treatment of 203 males with uncomplicated acute gonococcal urethritis. Cure rates above 95% were produced by all treatments except the 2.0-g amoxicillin dose, which cured 89% of patients. Of 198 pretreatment gonococcus isolates tested by an agar dilution technique for susceptibility to penicillin G, ampicillin and amoxicillin, over 50% showed relative resistance (MIC > 0.06 mug/ml) to the antibiotics. However, amoxicillin was somewhat more active against isolates showing considerable resistance (MIC >/= 1.0 mug/ml) to penicillin G or ampicillin. Adverse effects of amoxicillin were few: two patients reported transient nausea and six noted short-lived diarrhea. No hypersensitivity reactions were observed.


Radiotherapy for carcinoma of the cervix recurrent after surgery.


Coronary collateral flow following acute coronary occlusion: a diastolic phenomenon.


Nursing needs of ambulatory patients with chronic disease.


Structure-activity dependence in some novel ring-substituted 3,3-dimethyl-1-phenyltriazenes. Genetic effects in Drosophila melanogaster and in Saccharomyces cerevisiae by a direct and a host-mediated assay.


Comparative effects of commercial Aroclors on rat liver enzyme activities.


The synthesis of a DOPA analogue, 1-amino-2-(3, 4-dihydroxyphenyl)-ethylphosphonic acid and distribution studies in mice bearing the Harding-Passey melanoma.


The effect of aflatoxin B, on the maturation of ribosomal and transfer RNA in monkey kidney (CV-1) cells in culture.


Eye abnormalities and skeletal deformities in the Pierre Robin syndrome: a balanced evaluation.


Changes of individual proteins in mother and newborn.


Study of bile proteins in difuse chronic hepatopathies.


Electrophoretic forms of human liver alpha-L-fucosidase and their relationship to fucosidosis (mucopolysaccharidosis F).


The molecular nature of alpha-fetoprotein in anencephaly and spina bifida.


Biosynthesis of parathyroid hormone.

Biosynthesis of parathyroid hormone.


Lack of suppression of certain immunoglobulin-producing lymphocytes in T-lymphocyte deficiency.


Precipitating anti-adrenal antibodies in Addison's disease.


Proceedings: Dietary folates and their availability to man.


Micrognathia and glossoptosis in the newborn. Surgical tacking of the tongue in small jaw syndromes.


Proceedings: The intracellular pH and potassium content of rabbit cardiac and skeletal muscle in potassium depletion.


Characterization of a ribonuclease from the hepatopancreas of the queen scallop, Chlamys opercularis.


The J chain of polymeric immunoglobulins.


Some biochemical characteristics of the salivary gland products of Macaca irus and Cercopithecus aethiops.


Phylogenetic aspects of immunoglobulin variable region diversity.


mRNA for H and L chains of immunoglobulin: specific control of H-chain production.


Pyrbuterol: a new bronchodilator. Phase I-single dose study.


Penfluridol: a long-acting oral neuroleptic as therapeutic agent in chronic schizophrenia.


A system for the clinical assessment of the antitussive activity of caramiphen.


Local and systemic effects of beclomethasone inhalation in steroid-dependent asthmatic patients.


Hemodynamic effects of ethaverine hydrochloride in patients with peripheral vascular disease.


Arterial hypertension northwest Florida public health survey-I. Methodology.


Comparative bioavailability of doxycycline.


Hirschsprung's disease: its aetiology, pathogenesis and differential diagnosis.


[Normal body temperature and influencing factors].


Serum and liver lipids in normal baboons: effect of different carbohydrate diets.


Prostaglandins: current therapeutic status in obstetrics.


The effect of a single large dose of thyrotropin-releasing hormone on various aspects of thyroid function in the rat.


Proceedings: Treatment of chronic proctitis with disodium cromoglycate.


Proceedings: The influence of ethacrynic acid and aspirin on water and electrolyte secretion induced by prostaglandin E1 in the human jejunum.


Proceedings: Serum complement components C3 and C4 in inflammatory bowel disease.


Gel filtration of thyroxine - binding proteins of human serum.


Thyroxine content in the thyroid gland of domestic animals. Part VII. Iodinated tyrosines and thyronines in the serum of she camel at various reproductive stages.


The structure and metabolism of starch.

The structure and metabolism of starch.


Evidence in favour of the modification in vivo of RNA polymerase subunits during sporogenesis in Bacillus thuringiensis.


Active transport of maltose in Escherichia coli K12. Involvement of a "periplasmic" maltose binding protein.


A suppressor of nonsense mutations in Bacillus subtilis.


Correlation between the level of vitamin-B12-dependent methionine synthetase and intracellular concentration of vitamin B12 in some bacteria.


Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase.


Purification and properties of alpha-D-mannosidase from Medicago sativa L.


Protein A from Staphylococcus aureus. The effect of iodination on the reactivity with immunoglobulin G.


Inhibition by chloramphenicol of aminoazo dye carcinogenesis in rat liver: RNA synthesis in isolated liver nuclei.


The relative biological effectiveness of cyclotron fast neutrons for early and late damage to the small intestine of the mouse.


Histamine catabolism in guinea pigs, rats and mice.


Differences in papaverine inhibition of prostaglandin-induced contraction of aortic strips from normal and spontaneously hypertensive rats (SHR).


Change in cell-to-dish contact of cultured mammalian cells induced by concanavalin A.


Evidence for long-range electrostatic repulsion between HeLa cells.


Vitamin B12 and the macromolecular composition of Euglena. I. Kinetic analysis of the cell cycle and chloroplast replication.


Effect of bilateral intracerebral ablation on the gastric secretory response to insulin-induced hypoglycemia.


Retention of noradrenaline-3H in brain and preferential extraction of labeled metabolites by glutaraldehyde fixation.


Tremorogenic effect of thyrotropin releasing hormone in rats.


Determination of the LD01 and extrapolation of the LD001 for five methylcarbamate pesticides.


Enhancement of cerebral noradrenaline turnover by thyrotropin-releasing hormone: evidence by fluorescence histochemistry.


Reproductive incompatibility between similar genotype flies (Drosophila melanogaster) raised at two different temperatures.


Effect of sulpiride on prolactin release by rat pituitaries in vitro.


Studies on the influence of carboxyl-terminal amino acid residues on the activity and stability of human erythrocyte carbonic anhydrase B.


Formation of D-alanyl-D-alanine and D-alanine from UDPMurNAc-L-Ala-D-isoGlu-L-Lys-D-Ala-D-Ala by extracts of Staphylococcus aureus and Streptococcus faecalis.


Isolation and characterization of allophycocyanin II from the thermophilic blue-green alga Mastigocladus laminosus Cohn.


Immunological studies on the sigma subunit of the RNA polymerase from vegetative and sporulating cells of Bacillus subtilis.


Oxygen binding properties of Colus gracilis haemocyanin and its subunits.


Carbonic anhydrase-aromatic sulfonamide complexes, a resonance Raman study.


Correlation between pyocin-sensitivity and 2-amino sugar composition of Pseudomonas aeruginosa.


Computer-based auditory neurophysiology laboratory.


Induction of minute mutations in Drosophila by x-rays.


Genetic (h-2) dependence of the duration of splenomegaly induced by Brucella abortus.


Laboratory testing of some organic insecticides against Zyginidia quyumi (Ahmed) Typhlocybinae: (Cidadellidae) on maize.


The status of transplantation of the pancreas and pancreatic tissues as a cure for diabetes.


Protective antigens in bovine pasteurellosis.


Influence of nifedipine on the effort tolerance test in angina pectoris. Comparison with placebo and trinitroglycerine.


Nifedipine in angina pectoris. A multicentric clinical trial.


Phenotypic properties of R factors of Pseudomonas aeruginosa: R factors readily transferable between Pseudomonas and the Enterobacteriaceae.


Phenotypic properties of R factors of Pseudomonas aeruginosa: R factors transferable only in Pseudomonas aeruginosa.


Effect of anti-4-azoquinoline 1-oxide antibody on immunosuppression by 4-hydroxyaminoquinoline 1-oxide.


The use of bacteriophages for differentiating plasmids of Pseudomonas aeruginosa.


Genetic regulation and pattern formation: a study of the yellow locus in Drosophila melanogaster.


Genetic analysis of amidase mutants of Pseudomonas aeruginosa.


Regulation of enzyme activities in Drosophila. I. The detection of regulatory loci by gene dosage responses.


Regulation of enzyme activities in Drosophila. II. Characterization of enzyme responses in aneuploid flies.


Rubella antibody titer and IgG concentration in an aging population.


Supersensitivity to pentagastrin in diffuse oesophageal spasm. A 38-year-old patient, complaining of dysphagia and substernal pain of nine months' duration, was found by manometric studies to have diffuse oesophageal spasm. As has similarly been reported for cholinergic agents in achalasia and in some cases of diffuse oesophageal spasm the oesophageal body responded in a hypersensitive fashion to subcutaneous and intravenous injections of pentagastrin. This effect was inhibited by the intravenous administration of atropine and completely abolished by nitroglycerine. When pentagastrin was subcutaneously injected before a barium examination a distortion of the oesophageal contour, described as curling or corkscrew oesophagus, regularly appeared.


Some effects of a microcrystalline collagen preparation on blood.


Professor Frantisek Patocka, septuagenarian.


Effect of rifampicin and free amino acids on replication of DNA in Lactobacillus acidophilus.


Comparative studies of social behavior in Callicebus and Saimiri: behavior of male-female pairs.


Spores of microorganisms. XXV. The effects of histone on the syntheses of macromolecules in germinating and outgrowing spores of Bacillus cereus.


Male-male competition and infanticide among the langurs (Presbytis entellus) of Abu, Rajasthan.


Hematological development of the cebus monkey (Cebus albifrons and apella).


Harassment of sexual behavior in the stumptail macaque, Macaca arctoides.


Regulation of biosynthesis of excessive metabolites. XVI. Origin of the terminal group of tetracyclines.


Biological ensiling of sugarcane tops. VII. Isolation and characteristics of lactic acid bacteria.


Immunochemical studies on mannans of the genus Saccharomyces. Group of Saccharomyces sensu stricto species.


Properties of a phenolase preparation from cell suspension cultures of parsley.


Detection and isolation of a second acid stable proteinase inhibitor from human bronchial mucus.


Thymus proteases: extraction, distribution, comparison to lymph node proteases and species variation.


The amino acid sequences of the alpha and beta polypeptide chains of adult hemoglobin of the capuchin monkey (Cebus apella).


The interaction of native and chemically modified yeast transketolase with a specific antibody.


Alternative pathway for the activation of complement in human serum. Formation and composition of the complex with cobra venom factor that cleaves the third component of complement.


Studies on the primary structures of alpha and beta polypeptide chains of adult hemoglobin of the spider monkey (Ateles geoffroyi).


Glucose-6-phosphate 1-epimerase from bakers' yeast: purification, properties and possible biological function.


Glycogen metabolism in isolated perfused rat liver.


The molecular weight of J chains of human IgM.


Characterization of an acid-stable proteinase inhibitor in human cervical mucus.


Steroidglucuronyltransferases, V. Formation and hydrolysis of oestrogen glucuronides by the liver, kidney and intestine of the pig.


Biological, immunological and physical investigations on human chorionic gonadotropin.


The changing hospital economy.

The changing hospital economy.


Drug effects on vision: strategies for study and selected results.


Vitamin B12-like activity in leafy vegetables.


The genetic control of T cell-meditated immunity. I. Characterization of a mouse strain whose low responsiveness is inherited as a recessive trait.


The comparative serological response of the chicken, pheasant and quail to a soluble and a particulate antigen.


Quantification and distribution of chicken immunoglobulins IgA, IgM and IgG in serum and secretions.


Protection against Toxoplasma gondii in mice immunized with Toxoplasma cell fractions, RNA and synthetic polyribonucleotides.


Surface immunoglobulin of a mouse T-cell lymphoma.


Red cell Fc receptors and their participation in the passive haemagglutination mediated by non-precipitating antibodies.


Formation of salicylic acid from naphthalene by microorganisms: Part I. Studies on isolation, characterization & growth of bacterial isolates utilizing naphthalene.


Studies on the specificities of two IgM lambda cold agglutinins.


Formation of salicylic acid from naphthalene by microorganisms: Part II. Factors affecting salicylic acid accumulation.


Epizootiology of Kyasanur Forest Disease in wild monkeys of Shimoga district, Mysore State (1957-1964).


Studies on foetomaternal haemorrhage (FMH). Part I: Factors affecting foetomaternal haemorrhage.


Studies on foetomaternal haemorrhage (FMH). Part II. The role of placental pathology in foetomaternal haemorrhage.


Improved cell yield from monkey kidneys by using versene and trypsin.


Studies on the antibody response of a formalin inactivated Kyasanur Forest Disease Virus vaccine in langurs "Presbytis entellus".


Significant bacteriuria in apparently healthy young adults.


Mental development of children with severe protein calorie malnutrition in the past anbd present.


Investigation of secretory immunoglobulins in saliva from germfree mice. We investigated the effect of oral immunization on the serum and salivary immunoglobulin levels in axenic and conventional NIH mice. Specific antibacterial and antifungal antibodies were measured by passive hemagglutination, and differences in the immunoglobulin classes were measured by radial immunodiffusion. After an oral immunization regimen with either intact Escherichia coli or Candida albicans, a significant increase in specific antibody above the base-line reciprocal titer of 32 was observed in conventional mouse saliva. Saliva from axenic mice immunized with the formalized microorganisms yielded reciprocal antibody titers of 256, as compared with an absence of specific antibodies in control animals. Assay of the sera of both groups of immunized mice revealed no increase in antibody levels specific for E. coli lipopolysaccharide or Candidin.


Immunological study of typhoid: immunoglobulins, C3, antibodies, and leukocyte migration inhibition in patients with typhoid fever and TAB-vaccinated individuals. The development of humoral and cell-mediated immune responses to Salmonella typhi antigens and immunoglobulin and C(3) levels were determined in patients suffering from typhoid fever, TAB-vaccinated individuals, and appropriate controls. In 45 patients with typhoid, a significant elevation of immunoglobulin M (IgM) level was noted from the first week of illness onwards. Eighteen TAB-vaccinated persons also showed a significant elevation of IgM levels. In typhoid sera, the anti-O and anti-H antibodies were mostly 2-mercaptoethanol (2-ME) sensitive. The rise of IgM level correlated well with the 2-ME-sensitive anti-O and anti-H antibodies seen in typhoid patients. The anti-O antibodies in the TAB-vaccinated group were almost entirely 2-ME sensitive, but both 2-ME-sensitive and -resistant anti-H antibodies were detected in the TAB group. A marked increas in C(3) level was also noted in patients with typhoid. The cell-mediated immunity (CMI), as measured by leukocyte migration inhibition tests, was demonstrable in 15 of 22 patients with typhoid. On the other hand, only 8 of the 20 normal subjects, 5 of the 16 fever control cases, and 6 of the 18 TAB-vaccinated individuals gave a positive CMI. The latter three groups were comparable with each other but were significantly different from the typhoid patients. It was concluded that TAB-vaccination did not induce CMI even though it induced the development of antibodies, the latter being comparable with those of the patients with typhoid. The significance of these findings is discussed.


Some properties of precipitating antigens associated with infectious bursal disease virus. Infection of the bursa of Fabricius and chicken embryo fibroblast cell cultures with avian infectious bursal disease virus resulted in production of a number of virus-induced antigens. The antigens were specific, forming three precipitin lines by immunodiffusion with antiserum (designated PA-1, -2, and -3). To separate immunoprecipitin from the remaining viral particles, two (PA-1 and PA-3) were partially purified by subjection to two cycles of diethylaminoethyl-cellulose chromatography and filtration through a column of Sephadex G-150 gel. The precipitating antigen, PA-1, was found to migrate most slowly through the agar gel, remaining serologically active after treatment with heating (56 C for 1 h), trypsin, lipolytic solvents, deoxyribonuclease, and ribonuclease. Its density was 1.27 g/ml. Morphologically the antigen displayed a doughnut-shaped structure 8 to 12 nm in size. PA-3 migrated most rapidly through the agar gel. It was destroyed by treatment with heating and trypsin but not with lipolytic solvents, deoxyribonuclease, and ribonuclease. Density was about 1.25 g/ml. This suggests that the antigen is a part of viral structural components. PA-2 migrated through agar gel at a rate between that of PA-1 and PA-3. Because of its low concentration, PA-2 was not further characterized.


Interaction of staphylococcal exfoliative toxin with concanavalin A. Treatment of mouse embryo fibroblasts (MEF) grown in vitro with purified staphylococcal exfoliative toxin (ET) increased the concanavalin A (Con A) agglutinability of MEF 3.5-fold over control cells. Possible explanations for this phenomenon were investigated. ET lacked proteolytic activity on denatured casein. Con A, however, was found to interact directly with ET as evidenced by the formation of precipitation in an agar gel diffusion plate and in increased turbidity in solution. This interaction was inhibited by alpha-methyl-d-glucopyranoside. The ability of Con A to precipitate with ET suggests that the toxin contains a carbohydrate component and that the carbohydrate associated with ET is branched rather than linear. An analysis of a purified preparation of ET indicated the presence of 9% carbohydrate and no lipid.


Intracellular and extracellular degranulation of human polymorphonuclear azurophil and specific granules induced by immune complexes. The nature of the phagocytic stimulus determines the degree of degranulation. Specifically, immune complexes induced more degranulation than did antigen or antibody alone. The release of constituents from the two granule classes represented independent phenomena. Relatively more of the specific granule proteins were released into the medium during phagocytosis, whereas more of the azurophil proteins were associated with the phagosomes.


Immunological analysis of glycolipids and lipopolysaccharides derived from various mycoplasmas. The lipids and lipopolysaccharides of five mycoplasmas were examined for complement-fixing activity to antimembrane rabbit sera. Total glycolipid fractions and the aqueous phenol fractions (lipopolysaccharides) from the membranes of Acholeplasma laidlawii, A. modicum, A. axanthum, and Mycoplasma neurolyticum exhibited significant antigenic activity. The glycolipids and phosphoglycolipids from Thermoplasma acidophilum were either anticomplementary or did not react due to their extreme hydrophobic nature. High activity was found using the lipopolysaccharide of T. acidophilum. The pure glycolipids and phosphoglycolipids of the Acholeplasma and Mycoplasma species also exhibited significant complement-fixing activity. Acylation of the sugar residues of these lipids reduced or negated complement-fixing activity. Double cross-reactions between the glycolipids of A. laidlawii and A. modicum appeared to be due to mono- and diglucosyl diglycerides of identical structure. Specificity of glycolipid structure was noted by the absence of cross-reactions between A. laidlawii and M. neurolyticum, the glycolipids of which differ only in the nature of the glucose linkages. The existence of lipopolysaccharides in the membranes of mycoplasmas and their complement-fixing activity in the presence of antimembrane sera suggest their possible importance as specific antigenic determinants.


Modoc viral infections in the deer mouse Peromyscus maniculatus. The pathogenesis of Modoc virus and its mechanism of transmission were investigated in Peromyscus maniculatus gambeli (deer mouse) as a model to understand the natural history of this virus. Animals were readily infected by the intranasal or subcutaneous route of inoculation. Virus could be detected by direct isolation techniques in many organs and body fluids during the first 7 to 9 days after intranasal inoculation. Increases in viral titers were detectable first in lungs and then the spleen, salivary-submaxillary glands, and lymph nodes. Viremias were low titered and ephemeral. Virus was recovered from urine and throat swabs 4 to 6 days and 4 to 7 days after inoculation, respectively. Serum dilution neutralization, hemagglutination inhibition, or complement fixation antibodies were detected in sera of some animals 13 days after infection and in all animals after 20 days. Antibodies persisted for the 168-day period of observation. Persistent viral infection was demonstrable by in vitro culturing of lungs or pooled lungs, salivary glands, and kidneys from 14 of 69 mice that were sacrificed from 1 to 6 months after intranasal inoculation. Attempts to demonstrate horizontal or vertical transmission of Modoc virus among mice were generally unsuccessful. Female deer mice infected with virus before mating passed maternal antibodies to their young.


Opsonin system of the group B streptococcus. The opsonization by polymorphonuclear leukocytes of group B streptococcal serotypes associated with neonatal sepsis and delayed meningitis was studied. A specific BIa opsonizing antibody (not related to the antipolysaccharide typing antibody) was present in only 10% of the population tested. Serotype BIa was not opsonized in the absence of this specific antibody. BIa antibody specificity was demonstrated by macroagglutination and absorption with BIa streptococci and extracts, but not by gel diffusion. The binding of complement by the BIa opsonin increased the mean phagocytic activity by 60%; complement was manifested via the classic and/or alternate C3-related pathway, but seldom by both concurrently. Serotypes BIb, BIc, BII, and BIII were naturally and nonspecifically opsonized in 95% of the human and baboon sera or plasma tested. Although similar levels of opsonization were present in hyperimmune rabbit sera, heat inactivation and homologous bacterial absorption did not reduce the level of phagocytic activity in the rabbit, human, or primate groups studied. These immunological studies confirmed previous findings that serotype BIa presents a serious hazard for neonatal sepsis, with a nearly 100% mortality. Exclusive isolation of BIII in delayed meningitis suggests that ingestion and subsequent killing by polymorphonuclear leukocytes of type III may differ from the other serotypes.