Polychlorinated biphenyls: a new type of inducer of cytochrome P-448 in the liver. The CO-difference spectrum of microsomes from rats treated with the polychlorinated biphenyls mixture, Aroclor 1254, has an absorption maximum at 448 nm. With ethylisocyananide as the ligand for reduced microsomes, Aroclor 1254 treatment causes a shift in the 455-nm peak to 453 nm and increases the ratio of absorbance of 455 nm to that at 430 nm from 0.53, obtained with untreated rats, to 1.24. These findings are similar to those seen in rats treated with the polycyclic hydrocarbon, 3-methylcholanthrene, but differ from those that characterize cytochrome P-450 in control or phenobarbital-treated rats. Aroclor 1254 treatment results in a tripling of cytochrome P-448 content and a 10-fold increase in benzo-[a]pyrene hydroxylation. However-unlike 3-methylcholanthrene, but like the phenobarbital type of inducing agents-Aroclor 1254 treatment causes a significant enhancement of ethylmorphine N-demethylase. These data suggest that Aroclor 1254-induced cytochrome P-448 may be catalytically different from the 3-methylcholanthrene-induced P-448 or that the hemoprotein(s) induced by Aroclor 1254 may be a mixture of cytochromes P-448 and P-450 exhibiting catalytic properties of both cytochromes.


Regulation of the  -methylgalactoside transport system and the galatose-binding protein by the cell cycle of Escherichia coli. The synthesis of the periplasmic galactose-binding protein of E. coli is regulated by events occurring during its cell cycle, and proceeds in synchronized cells for only a short period after cell division is completed. Transport activity mediated by the beta-methylgalactoside transport system follows closely the synthesis pattern of the binding protein.A mutant, E. coli BUG-6, exhibits temperature-sensitive cell division [Reeve et al. (1970) J. Bacteriol. 104, 1052-1064], synthesizing galactose-binding protein at the permissive but not at the nonpermissive temperature. Galactose-binding protein synthesized at the permissive temperature is not degraded after the culture is shifted to the nonpermissive temperature. Polyacrylamide gel electrophoresis of the periplasmic proteins of BUG-6 grown at the permissive and nonpermissive temperatures suggests that several, but not all, periplasmic proteins are subject to the same regulatory control by the cell cycle as the galactose-binding protein.


Conformational analysis of thyrotropin releasing factor. Conformational energy calculations on thyrotropin releasing factor and on several of its analogues indicate that the central histidyl residue of the native molecule is in an extended conformation. Some derivatives (with a reduced biological activity) have an altered conformation. Since some substitutions leave the conformation unchanged but alter the biological activity, these substitutions must involve the sites responsible for binding of the hormone to its receptor.


Studies on in vitro DNA synthesis. Purification of the dna G gene product from Escherichia coli. varphiX174 DNA-dependent dNMP incorporation is temperature-sensitive (ts) in extracts of uninfected E. coli dna A, B, C, D, E, and G ts strains. DNA synthesis can be restored in heat-inactivated extracts of various dna ts mutants by addition of extracts of wild-type or other dna ts mutants. A protein that restores activity to heat-inactivated extracts of dna G ts cells has been extensively purified. This protein has also been purified from dna G ts cells and is thermolabile when compared to the wild-type protein. The purified dna G protein has a molecular weight of about 60,000, is insensitive to N-ethylmaleimide, and binds poorly to DNA. It does not stimulate heat-inactivated crude extracts of dna B, C, D, or E ts cells and lacks detectable RNA and DNA polymerase activities.


Attempted induction of sarcoma in a Cape Chacma baboon with the Snyder-Theilen feline sarcoma virus.


Features of cerebellar morphology and organization.


Multiple sclerosis: immunoglobulin alterations related to different clinical parameters.


The innervation of the cerebral arteries in the monkey.


Meningococcal infections.

Meningococcal infections.


Capillary basement membranes and diabetic microangiopathy.


Total parenteral nutrition.

Total parenteral nutrition.


Axon diameter and myelin thickness. Unusual relationships in dorsal root ganglia.


Study of the extracellular polysaccharides produced by a blue-green alga, Anabaena flos-aquae A-37.


Purification and properties of two forms of staphylococcal   toxin.


Drug-induced reinstatement of amphetamine self-administration behaviour in monkeys.


Water and ions in muscles and model systems.


Fundamentals of infant resuscitation.

Fundamentals of infant resuscitation.


Problems in the determination of polychlorinated biphenyls.


Decrease in pulmonary diffusing capacity during lipid infusion in healthy men.


The interaction of radioactive initiation factor IF-2 with ribosomes during initiation of protein synthesis.


Subunit heterogeneity of thyroglobulin.

Subunit heterogeneity of thyroglobulin.


The subunits of human pituitary thyroid-stimulating hormone. Isolation, properties, and composition.


The complete development of the sporogonous stages of Hepatozoon rarefaciens cultured in a Culex pipiens cell line.


Meningonema peruzzii gen. et sp. n. (Nematoda: Filarioidea) from the central nervous system of African monkeys.


Sarcocystis in dogs and its probable transmission from cattle.


Evolving concepts of thyroid function.

Evolving concepts of thyroid function.


The malabsorption syndrome.

The malabsorption syndrome.


Action of levallorphan: macromolecular synthesis and cell division.


Analogues of fusaric (5-butylpicolinic) acid as potent inhibitors of dopamine  -hydroxylase.


Levodopa and melanoma.

Levodopa and melanoma.


A new understanding of hepatitis.

A new understanding of hepatitis.


Somatic evoked response recording: an adequate test of deafferentation?


Induction of mating behavior in rats by luteinizing hormone-releasing factor. Ovariectomized female rats treated with estrogen, in dosages too low to provoke mating, displayed this behavior when given subcutaneous injections of synthetic luteinizing hormone-releasing factor (LRF) 48 hours later. Two hours after the injection of LRF, components of female sexual behavior appeared. The lordosis reflex followed mounting by the male, and darting and hopping behavior was quite prevalent. On the other hand, treatment with estrogen followed by luteinizing hormone, follicle-stimulating hormone, or thyrotropin-releasing factor did not induce copulatory behavior. The results suggest that LRF may play a role in induction of mating behavior.


Electron microscopic study of the endothelium of stored and cryopreserved monkey corneas.


Osteomyelitis in hemodialysis patients.

Osteomyelitis in hemodialysis patients.


Diabetic ketoacidosis associated with mumps virus infection. Occurrence in a patient with macroamylasemia.


Bilateral hilar adenopathy.

Bilateral hilar adenopathy.


How effective will national health insurance be?


Immunochemical studies of AMP:pyrophosphate phosphoribosyltransferase from normal and Lesch-Nyhan subjects.


Involvement of a carboxyl group in the active site of Bacillus cereus 569-H penicillinse ( -lactamase I).


Inhibition of phosphoenolpyruvate:uridine diphosphate N-acetylglucosamine enolpyruvyltransferase by uridine diphosphate N-acetylmuramyl peptides.


The effect of temperature, age and density of gametocytes, and changes in gas composition on exflagellation of Leucocytozoon simondi.


The fine structure of Leucocytozoon simondi. VI. Hepatic schizogony.


Corynebacterium diphtheriae in a patient with multiple trauma.


A clinical index of disability due to chronic asthma tested during a trial of cromolyn sodium treatment.


Short- and long-term prophylaxis with cromolyn sodium in chronic asthma.


An evaluation of bacterial contamination of ventilator humidifying systems.


Replication pattern and quinacrine fluorescence of the chromosomes of a CV-1-derived African green monkey cell line.


Complementation analysis of male fertility among the segregation distorter chromosomes of Drosophila melanogaster. The fecundities of 55 genotypes of the form SD(i)/SD(j) generated by 11 different SD chromosomes have been examined. Five of the genotypes are lethal The fecundities of the rest fall into a pattern of fertility and sterility that is highly suggestive of intracistronic complementation. The complementation leading to male fertility is only partial complementation: the fecundity of most fertile genotypes is less than half that of controls. The three components of the SD system, the Sd locus, the Ac locus, and the modifiers in 2R, were examined separately, and it appears that the complementation is a phenomenon associated with the Sd locus. A hypothesis of the molecular events involved in segregation distortion is formulated in the light of these observations. The model is based on the assumption that the Sd locus produces a multimeric molecule that regulates the activity of the Ac(=Rsp) locus during spermatogenesis.


Meiotic behavior of asymmetric dyads in the male Drosophila. Crossing over in the interstitial region of the heterozygous V(4) translocation in Drosophila melanogaster generates asymmetric dyads each consisting of a shorter and a longer chromatid. It was shown previously that in the female the shorter is recovered preferentially (Zimmering 1955); the present work suggests that the same occurs in the male as well. The mechanism in the female is envisioned as involving the non-random inclusion of the shorter chromatid into the functional egg (Novitski 1951); in the male, the two formal possibilities appear to be (1) that some proportion of sperm carrying the longer chromatid derived from an asymmetric dyad undergoes dysfunction, or (2) the longer chromatid is preferentially included into regularly-produced nonfunctional sperm, the nonfunctionality set up at the second division and distinguishing second division daughter cells.


Analysis of carbamates as fluorescent 1-dimethylamino-naphthalene-5-sulfonate esters.


Trace analysis and fundamental studies by plasma chromatography.


Culture for gonorrhea in asymptomatic college women.


Spectral sensitivity in primates: a comparative study.


Excretion of urate by the kidneys of Cebus monkeys: a micropuncture study.


Uricosuric effect of benziodarone in man and laboratory animals: a comparative study.


Plasma kallikrein-kinin system in nonmammalian blood: evolutionary aspects.


Nature of the uricosuric action of benziodarone.


Changes of plasma glucose and insulin during defense reactions in monkeys.


Recent advances in the physiology and pathophysiology of potassium excretion.


Release of thyroid stimulating hormone from chick anterior pituitary glands by thyrotropin releasing hormone (TRH).


Polygammaglutamyl metabolites of methotrexate.


A novel technique for the preparation of transport-active membrane vesicles from Pseudomonas aeruginosa: observations on gluconate transport.


The synaptic organization in the inferior pulvinar of the rhesus monkey (Macaca mulatta).


Sympathomimetic anesthetics.

Sympathomimetic anesthetics.


Radioimmunoassay of testosterone during fetal development of the rhesus monkey.


Comparison of baboon and human placental lactogens.


Suction flow drainage in head and neck surgery. A new principle for postoperative suction drainage.


Prenylamine lactate in angina pectoris.

Prenylamine lactate in angina pectoris.


Effect of nitrogen mustard and corticoids on experimentally produced villonodular synovitis.


Shigellosis in rhesus monkeys in quarantine.


Dimethyl sulfoxide in the treatment of experimental brain compression.


Use of CT1341 anaesthetic ("Saffan") in monkeys.


The cerebral autonomic nervous system. A proposed physiologic function and pathophysiologic response in subarachnoid hemorrhage and in focal cerebral ischemia.


Correlation between lethality and DNA single-strand breaks in Bacillus subtilis cells treated with N-methyl-N'-nitro-N-nitrosoguanidine.


Central neurogenic control of cerebral circulation. Effects of intravertebral injection of pyrithioxin on cerebral blood flow and metabolism.


Pulp capping in monkeys with a calcium-hydroxide compound, an antibiotic, and a polycarboxylate cement.


Myasthenia gravis. Cellular response to basic myelin protein compared with cellular and humoral immunity to muscle antigens.


Mechanisms of cholesterol accumulation in atherosclerotic lesions.


Nutrition and the liver.

Nutrition and the liver.


Growth and growth hormone in protein calorie malnutrition.


Serological analysis of wheat striate mosaic virus and its soluble antigen.


The role of sensitizing antibody in the neutralization of equine arteritis virus by complement or anti-IgG serum.


The fate of sensitized equine arteritis virus following neutralization by complement of anti-IgG serum.


Viral RNA synthesis in measles virus-infected cells.


Technical solution of the problem of investigation of instrumental conditioned reflexes with intragastric reinforcement.


Plasma and red blood cell amino acids of Egyptian children suffering from protein--calorie malnutrition.


Evaluation of small-scale nutrition programs.


Mode of action of rifampin on mycobacteria. I. Electron microscopic study of the effect of rifampin on Mycobacterium tuberculosis.


Correlation of in vitro and in vivo kinetics with clinical use of isoniazid, ethambutol, and rifampin.


The relative immunogenicity of virulent and attenuated strains of tubercle bacilli.


The fate of virulent and attenuated Mycobacteria in guinea pigs infected by the respiratory route.


Clearance and inactivation of the vegetative and spore forms of Bacillus subtilis Var niger in rat lungs.


Incidence of pulmonary tuberculosis.

Incidence of pulmonary tuberculosis.


A note on the toxic effects of tricresylphosphate on spinal ganglion of slow loris (Nycticebus cougang cougang).


Wheat leaf RNA polymerases. 3. Purification of the soluble RNA polymerase.


Permeability of the duodenum of the toad to non-electrolytes.


Australia (hepatitis-associated) antigen in patients attending a venereal disease clinic. A total of 1,650 patients attending the venereal disease department at St. Mary's Hospital, London, have been tested for Australia antigen. Twenty-three positive results were obtained, or 1.39%, which is more than 10 times the rate noted by others in blood donor populations in the U.K. and U.S.A. The positive rates among female patients and European male heterosexual patients were 0.36% and 0.19% respectively. High rates were obtained for homosexual patients (3.8%) and non-European heterosexual patients (3.1%). The reasons for the higher rates found in these groups merit further study.


Administration of disodium cromoglycate to young children.


Prognosis of acute renal failure.

Prognosis of acute renal failure.


Characterization and partial purification of  -hydroxybutyrate dehydrogenase from sporulating cells of Bacillus cereus T.


A cluster of mutations determining erythromycin resistance in Bacillus subtilis.


The immunological analysis of soluble human leukemia associated antigens.


Extracellular carbohydrate antigens from some non-pathogenic Neisseria species.


Immunological reactions of Mycobacterium leprae and Mycobacterium lepraemurium grown in cayman.


Improved techniques for assessment of plasma lipoprotein patterns. I. Precipitation in gels after electrophoresis with polyanionic compounds.


Immunochemical identification of prostatic tissue-specific acid phosphatase.


RIA: an historical note.

RIA: an historical note.


Objective assessment of a bronchodilator drug combination in asthmatic outpatients.


Lidoflazine and angina pectoris: a placebo-controlled double-blind study.


A study of the selectivity of action of oxyphencyclimine hydrochloride on gastric secretion. (selectivity of oxyphencyclimine).


A long-term follow-up of fluphenazine enanthate treatment.


Effects of hydrochlorothiazide in hypertensive patients and the need for potassium supplementation.


Milipertine: an early evaluation in severely ill schizophrenics.


SCH-12041 in the treatment of acute schizophrenic patients.


A double-blind comparison of AL-1021 and chlorpromazine in hospitalized schizophrenics.


In vitro studies of trans- and intraepithelial potentials of the cornea.


Recent studies on the nature and function of the corneal endothelial barrier.


Kinines and pain in "dry socket".

Kinines and pain in "dry socket".


Renal viability and oxygen consumption measurements during preservation.


Glutamic acid decarboxylase in spores of Bacillus megaterium and its possible involvement in spore germination. Spores of Bacillus megaterium were examined for glutamic acid decarboxylase (GAD). Although dormant spores showed no GAD activity, spores given sonic treatment and heat-activated spores had high activities when assayed for this enzyme. Several parameters of GAD in heat-activated spores were examined. The effects of KCN, NaN(3), 2,4-dinitrophenol, and KF on GAD activity were examined. Only KCN was an effective inhibitor of GAD activity in heated spores and was also shown to be the only effective inhibitor of GAD activity in vegetative bacteria. Similar patterns of inhibition were obtained with GAD activity and with spore germination, KCN being the only effective inhibitor of both, although at different concentrations. Spore GAD activity in heat-activated spores showed a loss with storage at 4 C; on the other hand, storage at 25 C was not accompanied by a loss, but, to the contrary, showed an increase in GAD activity of about 30%. A comparison of GAD activity at different times during germination, growth, and sporulation showed it to be highest in freshly germinated spores. Although vegetative cells contained GAD activity, the level in log-phase cells was approximately one-half the level obtained with freshly germinated spores. Heat-activated mutant spores with a requirement of gamma-aminobutyric acid for germination gave no GAD activity. GAD activity appeared in mutant spores after germination and increased to levels comparable to parent spores after 9 min of germination.


Deoxyribonucleic acid synthesis and deoxynucleotide metabolism during bacterial spore germination. Deoxyribonucleic acid (DNA) synthesis during germination of Bacillus megaterium spores takes place in two stages. In stage I (0-55 min) DNA synthesis is slow and there is no detectable net synthesis, whereas in stage II (from 55 min on) the rate of synthesis is much faster and net DNA synthesis occurs. Deoxyribonucleotide pool sizes match the rates of DNA synthesis in stages I and II. The level of deoxyribonucleotide triphosphates is not correlated with the level of deoxyribonucleotide kinases, but rather with that of ribonucleotide reductase activity.


Location of the SPO2 attachment site and the bryamycin resistance marker on the Bacillus subtilis chromosome. The attachment site on the Bacillus subtilis chromosome for the lysogenic bacteriophage SPO2 has been mapped by PBS1-mediated transduction and was found to be between spc-2 and lin-2, showing 90% linkage to the former and 30% linkage to the latter marker. In the course of these studies the bry-2 marker was mapped between the cysA14 and str-1 loci.


Osmotic behavior of bacterial protoplasts: temperature effects. Among protoplasts released from cells of Bacillus megaterium grown at 20, 30, or 37 C, osmotic swelling in NaCl solution at a given external osmotic pressure was greatest for protoplasts from cells grown at 20 C and least for protoplasts from cells grown at 37 C. Protoplasts from cells grown at lower temperaturs were also less stable to osmotic shock and lysed at higher external osmotic pressures than did protoplasts from cells grown at higher temperatures. But for cells grown at any one temperature, osmotic stabilization was itself temperature dependent so that the higher the ambient incubation temperature, the higher the osmotic pressure needed to prevent lysis of a given fraction of the input protoplast population. However, comparison of the osmotic stability of protoplasts from cells grown at different temperatures at various ambient incubation temperatures revealed that, except at 5 C where no differences were discerned, protoplasts from cells grown at lower temperatures still lysed at higher osmotic pressures than did those from cells grown at higher temperatures. The apparent internal osmolality (28 to 31 atm) did not vary significantly among whole cells from the three growth temperatures. Therefore, the observed differences in osmotic behavior could not be attributed to changes in internal osmotic pressure. Rather, it seemed likely that the differences were due to changes in membrane properties.


Isolation of two acetyl esterases from extracts of Bacillus subtilis. Acrylamide gel electrophoresis of crude cellular extracts of Bacillus subtilis revealed the presence of two acetyl esterases. Esterase A, the slower migrating enzyme, was found to be present in both vegetative and sporulating cells, whereas esterase B activity was more abundant after exponential growth ceased. Both esterases were present in the supernatant fraction of lysed spheroplasts and in a disrupted spore preparation. Of four pleiotropic asporogenous mutants tested, three exhibited decreased esterase B activity. Esterases A and B were partially purified by differential precipitation and co-chromatographed on diethylaminoethyl (DEAE)-cellulose (pH 7.5) and DEAE-Sephadex (pH 8.5). By employing gel filtration chromatography, the two esterases were separated, and molecular weights of 160,000 and 51,000 were estimated for esterases A and B, respectively. Esterase A was further purified to electrophoretic homogeneity by differential heating and preparative starch block electrophoresis. Sodium dodecyl sulfate-acrylamide gel electrophoresis of purified esterase A yielded a single protein band with a molecular weight of 31,000. The pI values of esterases A and B were determined to be 6.4 and 5.4, respectively.


Oxygen toxicity and the superoxide dismutase. Oxygen caused an increase in the amount of superoxide dismutase in Escherichia coli B but not in Bacillus subtilis. E. coli B cells, induced by growth under 100% O(2), were much more resistant to the lethal effects of 20 atm of O(2) than were cells which contained the low uninduced level of this enzyme. In contrast, B. subtilis, which could not respond to O(2) by increasing its content of superoxide dismutase, remained equally sensitive to hyperbaric O(2) whether grown under 100% O(2) or areobically. The catalase in these organisms exhibited a reciprocal response to oxygen. Thus, the catalase of E. coli B was not induced by O(2), whereas that of B. subtilis was so induced. These results are consistent with the view that superoxide dismutase is an important component of the defenses of these organisms against the toxicity of oxygen, whereas their catalases are of secondary importance in this respect. The ability of streptonigrin to generate O(2) (-), by a cycle of reduction followed by spontaneous reoxidation, has been verified in vitro. It is further observed that E. coli B which contain the high induced level of superoxide dismutase were more resistant to the lethality of this antibiotic, in the presence of oxygen, than were E. coli B which contained the low uninduced level of this enzyme. This difference between induced and uninduced cells was eliminated by the removal of O(2). These results are consistent with the proposal that the enhanced lethality of streptonigrin under aerobic conditions may relate to its in vivo generation of O(2) (-) by a cycle of reduction and spontaneous reoxidation. In toto, these observations lend support to the hypothesis that O(2) (-) is an important agent of oxygen toxicity and that superoxide dismutase functions to blunt the threat posed by this reactive radical.


Measurement of the cyanophycin granule polypeptide contained in the blue-green alga Anabaena cylindrica. An assay was developed to determine the amount of cyanophycin granules in blue-green algae. The amount of this polypeptide in cells of Anabaena cylindrica was measured as a function of culture age and was compared with changes in other proteinaceous cellular components. The data presented support the notion that the cyanophycin granule is a cellular nitrogen reserve.


Use of constructed double mutants for determining the temporal order of expression of sporulation genes in Bacillus subtilis. Double mutants containing two Spo mutations concerned with different stages of sporulation were constructed. In these, the phenotype that is exhibited is that of the earlier sporulation block. The same procedure was applied to sporulation mutants damaged in the same stage of development. The results provide a basis for placing in a temporal order different mutations concerned in stage II and stage IV of spore development. In general, the order indicated by the phenotypes of the double mutants is in agreement with the order derived on biochemical grounds. Double oligosporogenous mutants have also been constructed. Their sporulation incidence is roughly equal to the product of the incidences of the parent strains, idicating that separate factors are involved in overcoming each oligosporogenous block. The number of dependent sequential steps in sporulation is estimated as not less than about 12.


Mapping of asporogenous mutations of Bacillus subtilis: a minimum estimate of the number of sporeulation operons. Sporulation-specific mutations in Bacillus subtilis have been mapped by transduction and transformation. The mutations caused blocks at stages 0, II, III, and IV of sporulation; more than one phenotype was found for each of these stages. On the basis of the criteria used to define a sporulation operon, a minimum estimate could be made of the number of operons activated during sporulation. Nine operons were identified for stage 0, eight for stage II, five for stage III, and six for stage IV. It is probable that several of these 28 operons are activated in groups so that the number of steps in the dependent sequence of sporulation events should turn out to be less than the number of operons.


Surface structure of gliding bacteria after freeze-etching. Ultrastructural studies of gliding bacteria demonstrate 10- to 11-nm beads on the inner surface of the outer bilayer of Cytophaga columnaris. These were not found in Myxococcus xanthus. On treatment with glutaraldehyde and ethanol, the beads appear in linear arrays.


Prophylactic therapy of angina pectoris with organic nitrates: relationship of drug efficacy and clinical experimental design.


Pathogenesis of Marek's disease: early distribution of virus and viral antigens in infected chickens.


Extractable lipids of gram-negative marine bacteria: phospholipid composition. Phospholipid compositions of 20 strains of marine and estuarine bacteria were determined. Results showed that phospholipids of marine bacteria differed very little from those of nonmarine organisms with phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol being the predominant phospholipids in all strains examined. Lyso-phosphatidylethanolamine occurred in significant quantities among a number of the marine bacteria, and two of the isolates contained significant quantities of poly-beta-hydroxybutyrate. Effects of age and growth temperature on the phospholipid composition were also investigated. It is suggested that phylogenetic relationships among bacteria may be correlated with phospholipid composition.


Effects of d-amphetamine on schedule-controlled key pressing and drinking in the chimpanzee.


Urinary metabolic profiles for choosing test animals for chronic toxicity studies: application to naproxen.


Pharmacology of cyanate. I. General effects on experimental animals.


The long-stay hospital--a modern concept.


Metaplastic bone formation in the lung.

Metaplastic bone formation in the lung.


On the application of knowledge to the patient with genetic disease.


Encephalopathy and fatty degeneration of viscera: Reye's syndrome.


Cancer and the evolution of species: a ransom.


Subnuclear redistribution of DNA species in confluent and growing mammalian cells.


The skin in disseminated intravascular coagulation. Prospective analysis of thirty-six cases.


Determination of the binding of thyroxine to plasma proteins by competitive protein-binding analysis.


Polyacrylamide gel electrophoresis of human sera collected at different times after meals.


Evidence for a 5'-nucleotidase in human leukemic leukocytes.


Detecting Marek's disease herpesvirus: evaluation of biological systems.


Immunochemical study of relationships between erythrocyte glucose-6-phosphate dehydrogenase (G6PD, E.C. 1.1.1.49) of various mammalian species.


Microimmunodiffusion test for hemorrhagic enteritis of turkeys.


The biochemical properties of esterases in Cepaea (Mollusca: Helicidae).


Incorporation of  32 P during early amphibian embryogenesis.


Experimental induction heating hypophysectomy.


Properties of glycogen synthetase in erythrocytes.


Enzymic changes in taste buds of monkey following transection of glossopharyngeal nerve.


Protein A from Staphylococcus aureus. XIV. The effect of nitration of protein A with tetranitromethane and subsequent reduction.


Anatomical changes in peripheral nerves compressed by a pneumatic tourniquet.


A new preparative method for isolation of human C3 with affinity chromatography.


Immunochemical study of group A streptococcus peptidoglycan solubilized by 8M urea.


Histochemical evidence for phosphorylase, branching enzyme and glycogen synthetase activities in rat brain.


Death and dying. A critical review of the literature.


Analyses of proteinases in washing powders.


The retro-articular vertebral artery ring of the atlas and its significance.


Diverticulum or aneurysm of left ventricle.


Evidence for possible precursors in the synthesis of growth hormone by rat and human fetal anterior pituitary in vitro.


Inherited variations in thyroxine transport.


Basis of static and dynamic electrocardiographic variations in Wolff-Parkinson-White syndrome. Anatomic and electrophysiologic observations in right and left ventricular preexcitation.


The acylation of glycerol  3 -phosphate in different rat organs and in the liver of different species (including man).


Dysplasia epiphysialis hemimelica with confirmation by knee arthrography.


Regulation of growth hormone and prolactin secretion in patients with acromegaly and-or excessive prolactin secretion.


Visual discrimination performance in the monkey: the activity of single cells in infero-termporal cortex.


Certain ventromedial hypothalamic afferents.


Clinical grade, egional cerebral blood flow and angiographical spasm in the monkey after subarachnoid and subdural hemorrhage.


Gonorrhea: changing concepts in diagnosis and management.


Septic shock (endotoxic shock).

Septic shock (endotoxic shock). The pathophysiology, clinical aspects, medical, and surgical management of endotoxin shock are reviewed.  In the primate, the pathophysiology of endotoxin shock is contributed to by selective vasopasm, disseminated intravascular coagulation, and reduced myocardial response to sympathetic stimuli.  Studies in the baboon measured various parameters of hemodynamics and coagulation, catecholamines, and some biochemical changes following the injection of a single bolus of endotoxin.  Hemodynamic studies pointed to the kidney as a primary target organ.  Coagulation changes included alterations in factor XII and XIII (and others) and plasminogen.  Deposition of fibrin was also noted.  Neurohormonal studies using tritiated norepinephrine showed a sharp rise in catecholamines 3 minutes after injection of endotoxin followed by a return to normal within 120 minutes, confirming the role of vasopasm in reducing renal perfusion early in shock.  Prevention of septic shock is the best way to eradicate the extremely high reported mortality rates; infected abortion, chorioamnionitis, and pyelonephritis should all be warning signals.  Methods of monitoring the patient in septic shock with special attention to blood pressure, central venous pressure, blood volume changes, and urinary output are discussed.  Early surgical intervention and the proper use of vasomotor drugs and corticosteroids enhance patient survival.


A proposed role for ascorbate in the transport of amino acids and ions in the cornea.


The toad's (Bufo marinus) lens and urinary bladder as mural membranes: a comparison.


6-Azauracil induced phenocopies of rudimentary wing mutants and their suppression by a suppressor mutant of rudimentary in Drosophila melanogaster.


Somatic resistance to X-ray treatment in Drosophila melanogaster populations with different irradiation backgrounds.


Complement: optimal reaction conditions for guinea pig C4.


In vitro IgM antibody synthesis by human tonsil-derived lymphocytes.


Complement: a reversible temperature-dependent alteration in the reactivity of EAC1 with C4.


Lysosomal enzyme release from human leukocytes: mediation by the alternate pathway of complement activation.


Antigen-T lymphocyte interactions: inhibition by cytochalasin B.


Hyperlipidemia and diabetes mellitus: can electron microscopy help in the diagnosis.


Regulation of ribonucleic acid synthesis in Escherichia coli B-r: an analysis of a shift-up. II. Fraction of RNA polymerase engaged in the synthesis of stable RNA at different steady-state growth rates.


Occurrence of heat-dissociable ribosomal RNA in insects: the presence of three polynucleotide chains in 26 S RNA from cultured Aedes aegypti cells.


Response to noise of auditory nerve fibers in the squirrel monkey.


Role of abducens neurons in vestibuloocular reflex.


Location of cells of origin of spinothalamic tract in lumbar enlargement of the monkey.


The histopathology of vesical diverticula.


Neoplasm in vesical diverticula: report of 4 patients, including a 21-year-old.


Isolation, purification and structure of Aleutian disease virus by immunological techniques.


Does the rDNA in the Y chromosome of Drosophila melanogaster magnify?


Neural control of social behavior: prefrontal and anterior temporal cortex.


Functional dissociation between the inferior and arcuate segments of dorsolateral prefrontal cortex in the monkey.


Cross-modal transfer between vision and touch of go, no-go discrimination learning in the monkey.


Chlorthalidone in the treatment of angina pectoris.


Effect of trisodium nitrilotriacetate (Na 3 NTA) on the metabolism of selected metal ions.


Toxicity of (4,4'-(isopropylidenedithio)bis(2,6-di-t-butylphenol)), probucol, in mice, rats, dogs and monkeys: demonstration of a species-specific phenomenon.


Intestinal absorption of disodium ethane-1-hydroxy-1,1-diphosphonate (disodium etidronate) using a deconvolution technique.


Sensitization to epinephrine-induced ventricular fibrillation produced by probucol in dogs.


Effect of aflatoxins on microorganisms.

Effect of aflatoxins on microorganisms.


Hemodynamic changes during complete heart block in the unanesthetized monkey.


Detection of medullary thyroid cancer by calcitonin assay in families.


Asthma caused by nickel sensitivity.

Asthma caused by nickel sensitivity.


Rapid correction of hyponatremia in the syndrome of inappropriate secretion of antidiuretic hormone. An alternative treatment to hypertonic saline.


National health plans: some economic and financial aspects.


The early phase of acute myocardial infarction: pharmacologic aspects of therapy.


Cromolyn prophylaxis for chronic asthma.


Alcoholism and ketoacidosis.

Alcoholism and ketoacidosis.


Effects of bacterial products on a Paramecium esterase.


Electron microscopic studies on transplantable melanotic and amelanotic melanomas in hamsters.


Studies of a homologous gene-enzyme system, esterase C, in Drosophila melanogaster and Drosophila simulans.


The relationship of substrate, growth rate, and maintenance coefficient to single cell protein production.


Is hemoglobin A 2  important?

Is hemoglobin A 2  important?


A method for the measurement of platelet adhesiveness by use of dialysis membranes in a test-cell.


Polysomes of cultured normal and leukaemic lymphocytes: response to phytohaemagglutinin.


Body potassium and fat-free mass.

Body potassium and fat-free mass.


The effect of anticonvulsant therapy upon the absorption of folates.


Effects of long-term feeding of polychlorinated biphenyls (PCB, Clophen A 60) on the length of the oestrous cycle and on the frequency of implanted ova in the mouse.


Multiple myeloma proteins: evaluation of laboratory methods as aids for diagnosis.


Transmissible mink encephalopathy: studies on the peripheral lymphocyte. Circulating lymphocytes from mink with transmissible mink encephalopathy were studied for the presence of virus particles and for infectivity. Electron microscope examination of phytohemagglutinin-stimulated lymphocyte cultures revealed no evidence of virus-like particles. Circulating lymphocytes were also found to be noninfectious when the transmissible agent could be demonstrated in intact lymphoid organs in infected mink. A lymphopenia was detected in affected mink during the late clinical stage of disease, but this was most likely a secondary effect due to a terminal stress factor.


Studies on the virulence of Neisseria gonorrhoeae. I. Relation of colonial morphology and resistance to phagocytosis by polymorphonuclear leukocytes. Colonial varieties of Neisseria gonorrhoeae that are associated with virulence, types 1 and 2, were more resistant to phagocytosis by rabbit exudative polymorphonuclear leukocytes than colonial types of lesser virulence, types 3 and 4. Type 1 bacteria were resistant and type 4 gonococci were susceptible to phagocytosis by human polymorphonuclear leukocytes. Recent local type 1 isolates were similar in resistance to type 1 organisms of a standard laboratory strain (F62). Living and Formalin-treated, heat-killed, type 1 gonococci were equally resistant to phagocytosis. The antiphagocytic property of virulent colonial types was independent of leukotoxic action. Phagocytosis of both type 1 and type 4 gonococci by rabbit and human leukocytes was bactericidal. Rabbit leukocytes were superior to human leukocytes in killing gonococci. The results suggest that N. gonorrhoeae has virulence properties similar to those of extracellular bacterial pathogens, i.e., virulence is associated with antiphagocytic properties.


Mumps class-specific immunoglobulins in radioimmunoassay and conventional serology. In assessing the host cell range of bovine parvoviruses, these viruses were found to replicate optimally in actively dividing bovine fetal lung and spleen cells. Other primary bovine fetal cells supported growth to a lesser extent, but bovine line cells and line cells of other animal species tested did not. Minimal infectivity remained after passage of bovine parvovirus in cells from chicken embryos and guinea pig fetuses. During bovine parvovirus replication in bovine fetal lung and spleen cells, production kinetics of infectious virus and hemagglutinins were determined. An eclipse period of 16 h occurred, and viral release from cells was not detected until 30 h after inoculation of bovine fetal lung cells and 36 h after inoculation of bovine fetal spleen cells. Cell-associated virus titers were always higher than extracellular virus titers. Hemagglutinins were detected in parallel to infectious virus.


Interaction of virulent and avirulent Listeria monocytogenes with cultured mouse peritoneal macrophages. The interaction between smooth and rough Listeria monocytogenes and mouse peritoneal macrophages in culture was investigated. Initially, antibiotics were deleted from the culture medium, and no attempt other than the removal of unphagocytized bacteria by extensive washings was made to control extracellular growth. Under these conditions the monolayers were rapidly destroyed within an 8-h period, and this was associated with increases in the intracellular population of both strains. Extracellular viability counts revealed that washings failed to reduce the bacteria in the medium to less than 10% of the original inoculum. Continuous phagocytosis of Listeria which grew logarithmically in the maintenance media appears to account for the observed changes in the number of intracellular bacteria. The data also indicate that it is primarily the free bacteria in the culture medium which are responsible for the cytotoxic effects. In other experiments streptomycin-penicillin solutions were added to the maintenance media after an initial period of phagocytosis. In the presence of antibiotics, the total number of macrophages per field remained relatively constant, and no morphological alterations in the leukocyte cultures were observable. Extensive intracellular multiplication of either strain was not evident in fixed and stained cover slips. Viable intracellular counts reveal that after 24 h there is almost total killing of the rough variant, whereas the smooth strain tended towards complete survival.


Effects of antithymocyte and antimacrophage sera on the survival of mice infected with Listeria monocytogenes. Antisera prepared in rabbits against purified mouse thymocytes (antithymocyte serum; ATS) and peritoneal macrophages (antimacrophage serum; AMS) were injected intraperitoneally into Balb/c mice infected with the bacterium Listeria monocytogenes. When administered near the initiation of infection, the ATS significantly decreased the survival time of the animals and increased the mortality rate. When ATS was administered 6 days after a sublethal dose of L. monocytogenes had been inoculated, an overt disease did not evolve. ATS that significantly potentiated primary listeriosis also had high cytotoxicity titers for thymocytes and lymphoid cells from the peritoneal cavity. Although cytotoxic activity against peritoneal macrophages could be demonstrated in lower dilutions of the ATS, this activity did not appear to correlate with the effects of the sera on listeriosis. The injection of AMS did not enhance the infectious process. In some trials more deaths occurred among mice receiving normal rabbit serum than those receiving AMS. All of the AMS had cytotoxic titers against peritoneal macrophages, and the sera were usually inactive against thymocytes and peritoneal lymphoid cells. Listeria was isolated from fatally infected mice with nearly equal success in all of the serum-treated groups, and the serum treatments did not appear to alter the pattern of gross lesions. The afferent limb of the immune response was markedly affected by the presence of antibodies to lymphocytes. However, antibodies reacting with macrophages did not demonstrably enhance the Listeria process, which depends upon cellular immunity as the principal means of acquired host defense.


Nonhemolytic group B streptococci of human, bovine, and ichthyic origin. The biochemical and serological characteristics of nonhemolytic group B streptococci of human, bovine, and ichthyic origin were described.


Salivary, nasal wash, and sputum IgA concentrations in atopic and nonatopic individuals.


Stimulation of DNA synthesis and 2-deoxy-D-glucose transport in chick embryo cultures by excessive metal concentrations and by a carcinogenic hydrocarbon.


Ossifying fibroma of mandible with a case report.


Kidney transplantation in Fabry's disease.


Committee on maternal welfare.

Committee on maternal welfare.


Cerebral cortical control of movement.

Cerebral cortical control of movement.


Cardiovascular observations in the Macaca nemestrina monkey in Biosatellite 3.


Circadian rhythms and sleep-wake activity in the Biosatellite monkey.


Electrolyte and metabolic problems of weightlessness.


Wake and sleep cycles in the Biosatellite 3 primate in earth orbital flight.


The prospect of life on Jupiter.

The prospect of life on Jupiter.


Isolation and characterization of a virus infecting a blue-green alga of the genus Synechococcus.


Aspects of the developmental morphology of California encephalitis virus in cultured vertebrae and arthropod cells and in mouse brain.


Accommodation of the eye during sleep and anesthesia.


The oscillatory waves of the primate electroretinogram.


Retinal receptive fields: correlations between psychophysics and electrophysiology.


Servo-controlled moving stimulus generator for single unit studies in vision.


Field trial of the efficacy of a previously proposed regimen using minocycline and rifampin sequentially for the elimination of meningococci from healthy carriers.


Induced hypothermia: its place in modern hospital practice.


Massive hemorrhage associated with circulating antibodies to factor V.


Experimental calcification of the myocardium. Ultrastructural and histochemical investigations. Focal areas of calcification are frequent in rat myocardium 30 and 60 days after administration of dihydrotachysterol. These areas are PAS-positive, stain deeply with alcian blue and show high affinity for colloidal iron. Calcification is almost completely confined to intracellular structures. Small clusters of needle-shaped crystals are first found in apparently undamaged mitochondria in undamaged myocardial cells. When all the mitochondria are calcified, the cell degenerates, and inorganic crystals are laid down in relationship with its myofilaments. In other myocardial cells, clusters of amorphous or finely granular inorganic substance are found in both mitochondria and myofibrils. Both structures show signs of advanced degeneration. Inorganic substance has only occasionally been found within the structures of the sarcoplasmic reticulum. These structures do not seem to be involved in myocardial calcification under the present experimental conditions. Calcification of myocardial cells gives rise to a cellular reaction. Many macrophagic cells surround the calcified areas, which are rapidly reabsorbed. The present results show that myocardial mitochondria are actively engaged in controlling the intracellular concentration and movement of calcium ions. Their role in the myocardial contraction-relaxation cycle and the possible mechanism of myocardial calcification are discussed.


Progress in oral therapy of diabetes mellitus with sulphonylureas of the second generation.


Representation of information about skin temperature in the discharge of single cortical neurons.


Formylation of folate as step in physiological folate absorption. Oral administration of folate analogues to rats is followed by a rise in plasma folate detectable only by micro-biological assay with Lactobacillus casei, suggesting methylation of folate during absorption. When using the everted rat gut technique with folate (PteGlu), dihydrofolate (H(2)PteGlu), or tetrahydrofolate (H(4)PteGlu) on the mucosal surface formyl folate (10-CHO-PteGlu, 10-CHO-H(4)PteGlu, 5-CHO-H(4)PteGlu) and methylfolate (5-CH(3)-H(4)PteGlu) are recovered from the serosal fluid. This indicates that absorption of PteGlu is followed by formylation and that the formyl group is further reduced to methyl, 5-CH(3)-H(4)PteGlu passing on to portal blood.


Euglycaemic diabetic ketoacidosis.

Euglycaemic diabetic ketoacidosis. Of a series of 211 episodes of diabetic metabolic decompensation 37 had severe euglycaemic ketoacidosis (a blood sugar level of less than 300 mg/100 ml and a plasma bicarbonate of 10 mEq/1. or less). All were young insulin-dependent diabetics, only one being previously undiagnosed. Vomiting was a common factor, and in all carbohydrate reduction occurred with continued or increased daily insulin dose. Treatment comprised fluid and electrolyte replacement and large doses of insulin covered by adequate carbohydrate, many receiving 10% dextrose. Alkali was either withheld or given sparingly and the therapy was monitored by serial estimations of plasma bicarbonate. All the patients survived.


, Mishkin M:Comparison of superior temporal and inferior prefrontal lesions on auditory and non-auditory tasks in rhesus monkeys.


Cutaneous receptive fields of primate cold fibers.


Studies on isolated keratin fractions from mammalian epidermis. II. Immunological and immuno-electrophoretic comparison of the fractions.


Interactions of sensory and nonspecific thalamic inputs to cortical polysensory units in the squirrel monkey.


Position sense after combined spinal tractotomies and cerebellectomies in macaques.


B-lymphocyte mediated cytolysis: a complement independent phenomenon.


Incidence of Australia antigen in voluntary and professional blood donors and also in cases of viral hepatitis.


Specific hapten binding activity in normal sera.


Cost accounting of health centre expenditures.


Airway problems in the intensive care unit.


Micromethod for immunochemical quantitation of dextran and studies on role of antigen size in single radial immunodiffusion.


Lysis of antibody-treated human erythrocytes by human leukocytes and macrophages in tissue culture.


Defined immunofluorescence studies of cytoplasmic antibodies.


Ambient light, white noise, and monkey vocalization as sources of interference in visual short-term memory of monkeys.


Comparative study of myocardial lesions in hemorrhagic shock.


Spectrofluorometric determination of substituted dihydroisoquinolines and tertiary tetrahydroisoquinolines as their isoquinolinium derivatives.


Effects of pressure gradient changes on the morphology of arachnoid villi and granulations of the monkey.


Capacitor electrode stimulates nerve or muscle without oxidation-reduction reactions. Porous tantalum disks, available as "slugs" from the capacitor industry, have large available surface area and a thin insulating coating of tantalum pentoxide. When implanted, they fill with extracellular fluid and operate as capacitor-stimulating electrodes having high capacitance per unit volume. Capable of stimulating excitable tissute without generating electrochemical by-products, these electrodes should provide a safer interface between neural prosthetic devices and human tissue.


Renal blood flow during endotoxin shock in the subhuman primate.


Elemental diet in the treatment of fistulas of the alimentary tract.


The ocular manifestations of congenital rubella.


Safe use of pesticides.

Safe use of pesticides.


Status of monosodium glutamate revisited.


Folate content of diets in pregnancy: comparison of diets collected at home and diets prepared from dietary records.


The neglect of nutrition and its perils.


Nutrition: its relationship to children's physical, mental, and emotional development.


Graded contractions in muscle strips and single cells from Bufo marinus stomach.


Variation in endotoxin-induced kinin production and effect between the rabbit and rhesus monkey.


Toxicology of radionuclides.

Toxicology of radionuclides.


Heterotopic ossification in abdominal operation scars.


Neuropharmacology of the affective disorders.


Spondylo-metaphyseal dysplasia.

Spondylo-metaphyseal dysplasia.


Dyggve disease.

Dyggve disease.


Scleromalacia perforans associated with Crohn's disease. Treated with sodium versenate (EDTA).


Increased dosage of disodium cromoglycate.


The effect of D-L-erythro- - -dihydroxybutyraldehyde (DIBA) on the spores of Bacillus cereus: germination, stimulation and outgrowth inhibition.


Efficacy of glibenclamide in maturity-onset diabetics as maintenance therapy.


Effect of oxytocin and epinephrine on the adenylate cyclase activity of myometrium from pregnant Rhesus monkeys.


Fixed-interval schedule of cocaine reinforcement: effect of dose and infusion duration. Rhesus monkeys were trained on a fixed-interval 9-min limited-hold 3-min schedule of intravenous cocaine reinforcement. A 15-min timeout followed each reinforcement or limited-hold expiration. An identical schedule of food reinforcement was interspersed in the session to assess rate-modifying effects of the drug infusions not specific to drug reinforcement. In one experiment, response rate for cocaine reinforcement was shown to be a positive function of reinforcement magnitude for a dose range from 0 to 800 ug/kg/inj. At these doses, there was little effect on food reinforced responding except at the highest dose, where responding decreased. Results of the second experiment indicated that increasing the duration of the cocaine infusion produced a change in response rate similar to decreasing unit dose. The response rate change for a given increase in infustion duration was less at a unit dose of 400 ug/kg than at 200 ug/kg.


Long-term prereward suppression in monkeys unaccompanied by cardiovascular conditioning. In Experiment I, a 3-min tone that preceded a free pellet of food suppressed variable-interval performances maintained by the same type of pellets, but failed to elicit conditioned changes in the heart rates and blood pressures of two rhesus monkeys. Initially severe, the prereward suppression became temporally discriminated to progressively later portions of the tone, and was maintained at an attenuated level for over four months. The suppression was apparently not caused by interfering autonomic respondents, nor was it superstitiously conditioned, since 21 of the initial 25 tone-food pairings took place outside of baseline sessions. In Experiment II, a 1-min light, paired with four free pellets of food, suppressed the variable-interval responding of a second pair of similarly trained monkeys. An interresponse-time analysis showed that in one subject, mild prereward suppression of responding developed through two stages. On early trials, response rate slowed by 10% throughout the prefood interval. On later trials, the animal suppressed by pausing for a like portion of the interval, most often near the end, but otherwise responded normally during the prefood signal.


The chronic toxicity of retrorsine, a pyrrolizidine alkaloid, in vervet monkeys.


Use of thyrotrophin releasing hormone test in pituitary disease.


Effect of pyrithioxine (Encephabol) on growth and exploratory behaviour of rats malnourished in early life.


The nature of performance deficity under secobarbital and chlorpromazine in the monkey. A behavioral and EEG study.


Studies on gold therapy with  198 gold sodium thiomalate.


Prevention by cycloheximide of neutral red-induced formation of autophagic vacuoles and krinom granules in mouse pancreatic acinar cells.


Morphological studies of cultured swine aorta media explants.


Effects of anti-thymocyte serum on the phagocytic acivity of hepatic and splenic macrophages.


Intranuclear mitochondriae in Hodgkin's disease.


The centrineurogenic etiology of the acute respiratory distress syndromes. Universal, species--independent phenomenon.


Economic pressures--and quality nursing.


Tricuspid valvulectomy without prosthetic replacement.


Antibacterial effect of an interferon-inducing polycarboxylate. Mechanism of action.


Detection of homocytotropic human antibodies by passive sensitization "in vitro" of monkey lung tissue. I. Optimum conditions fo use of lung tissue.


Fixation of albumin to the hinge region of a macroglobulin.


Miliary tuberculosis in British Columbia. The case records were studied of all 48 patients with miliary tuberculosis registered by the British Columbia Division of Tuberculosis Control between 1967 and 1971. Two groups of patients were found, one group of 30 in whom the diagnosis was made during life and antituberculous therapy instituted, and a second group of 18 patients in whom the diagnosis was made only at autopsy and who had received no antituberculous therapy. In the latter group there was a particularly high incidence of concomitant disease; additionally, tuberculin testing, bacteriological search for acid-fast bacilli and chest radiography were frequently neglected. This group consisted mainly of elderly male Caucasians. The disease was characteristically insidious in onset, frequently with non-specific symptoms and physical findings. Fever was a common presenting symptom. Several patients presented with a fulminant illness which, although diagnosed, was not affected by antituberculous therapy and was rapidly fatal. North American Indians showed a high incidence of disease but a low mortality rate, probably owing to their younger age.


Analysis of lactic dehydrogenase in the lethal toad hybrid Bufo americanus female times Bufo marinus male.


The prophylaxis of nonindustrial urothelial tumours. Present knowledge concerning carcinogenesis and the natural history of urothelial tumours precludes firm conclusions relative to nonindustrial prophylaxis. However, a number of measures are consistent with current data and may be instituted for those patients with a demonstrated propensity to urothelial tumours. Their acceptability is based on the lack of associated toxicity for the patient. These measures include the elimination of significant infection, cigarettes, artificial sweeteners, analgesic abuse and coffee, the administration of vitamins C and B(6), and in selected cases, the use of thiotepa. It is emphasized that the merit of these steps in altering the natural history of urothelial tumours is uncertain.


Avian hepatic esterases, pesticides and diet.


Pyruvate kinase isozymes: a comparative study in tissues of various mammalian species.


The effects of washing, EDTA, magnesium and calcium on oxidative phosphorylation and respiratory rates of mitochondria from heat- and cold-acclimated rats.


Australia antigen and primary biliary cirrhosis. Sera from 64 patients with primary biliary cirrhosis have been examined for Australia antigen (Au). On immunodiffusion and immunoelectrophoresis all sera were negative. Using a radioimmunoprecipitation technique 15.6% of sera contained antigen compared with an incidence of 3.1% in matched controls, a significant difference (p = 0.015). Anti-Au was found in 9.4% of patients and in 7.8% of controls. In lymphocyte transformation studies lymphocytes from one of 24 patients with primary biliary cirrhosis transformed on stimulation with an Au-rich serum.


Electrofocusing of hepatitis B antigen and possible applications to radioimmunoassay techniques.


Phosphorylase activity in the arterial wall.


Association of white cell and red cell antibodies in human sera. Five hundred and eighteen human sera containing known red cell antibodies were tested for lymphocytotoxic antibodies and 81 sera were found to contain them. Thirty-nine antibodies were fully characterized. The frequencies of anti-I, K, V(w), and Wr(a) were significantly greater in those of the 518 sera which also contained white cell antibodies.Four hundred and ninety-four of the 518 sera containing red cell antibodies contained anti-Rh and anti-Kell. The frequency of white cell antibodies in this group was 15% compared with a frequency of 12% in a series of 923 antenatal samples not containing anti-Rh or anti-Kell. The frequencies of different anti-HL-A specificities were compared in the two groups with or without anti-Rh and anti-Kell antibodies. Anti-HL-A 1, 7, and 8 occurred more frequently in the absence of these red cell antibodies and anti-HL-A 12 occurred more frequently in their presence. No correlation was found between particular red cell and white cell antibodies.


Carcinogenic activity of benzofuran and dibenzofuran analogs of p-dimethylaminoazobenzene.


Epidemilogy of Pseudomonas in a burn intensive care unit.


Eukaryotes-prokaryotes divergence estimated by 5S ribosomal RNA sequences.


Enhancement of ampicillin efficacy by peroral levodopa in rat pyelonephritis.


On the mode of action of BCG.

On the mode of action of BCG.


Prospects for research on schizophrenia. 3. Neurophysiology. Isolation-rearing in primates.


Prospects for research on schizophrenia. 3. Neurophysiology. Primate behavioral pathology under field and laboratory conditions.


Prospects for research on schizophrenia. VII. Prospects for research in schizophrenia. An overview.


Combined bactericidal activity of sodium sulphacetamide and cetrimonium bromide.


Unreduced dislocations.

Unreduced dislocations.


Personal monitoring device for gaseous contaminants.


Stability of nitroglycerin in aqueous solution.


Neurofibromatosis in pregnancy.

Neurofibromatosis in pregnancy.


Photon fluence calculations for estimating dose distribution in the thorax.


Effects of Ancrod (Arvin) in mice: studies of plasma fibrinogen and fibrinolytic activity.


The control of radiation caries.

The control of radiation caries.


Epilepsy following cerebral abscess. A clinical and EEG study of 70 patients.


Generalized sharp and slow wave complexes. Associated clinical features and long-term follow-up.


The mechanism of drainage of the cerebrospinal fluid.


Histological changes of the pituitary gland in cockerels bearing basal hypothalamic lesions and demonstration of its anti-HCG immunofluorescence reacting cells.


Effect of glomerulopressin, oxytocin, and norepinephrine on glomerular pressure in the toad.


Failure of mammalian TRH to stimulate thyroid function in the lungfish.


Results from evaluating a Canadian regional mental health program.


Fallcies in using length-of-stay and rturn rates as measure of success.


The unit system: current staff attitudes and future directions.


Isolation and characterization of a rabbit adrenal autoantigen.


Physicochemical characterization and isolation of rabbit kidney-specific autoantigens.


Isolation and characterization of a rabbit non-tissue specific autoantigen which is shared by kidney, heart, adrenal and liver.


Identification of human reagins isolated with immunosorbent as IgE.


Induced homocytotropic antibody to bovine serum albumin in nonatopic dogs.


Linkage of thyroxine-binding globulin deficiency to other X-chromosome loci.


Location of inexchangeable sodium in the nucleus and cytoplasm of oocytes of Bufo bufo exposed to sodium-free solutions. 1. In oocytes exposed to Ringer solution in which Li substitutes for Na, 13-62% Na is inexchangeable with Li.2. Nuclei of oocytes isolated by dissection in salt solutions swell irrespective of the concentration or ionic components of the solution. When isolated in 4% bovine albumin solutions, swelling is negligible.3. When the nuclei of cells exposed to Li are isolated in 4% albumin solution, less than 6% of the inexchangeable Na is found in the nucleus, while 36-88% of it is found in the cytoplasmic fragments remaining after removal of the nucleus.4. When a Li-exposed cell is crushed in a cellophane bag and dialysed against Ringer or Li-substituted Ringer, 86-92% of the inexchangeable Na diffuses out.5. It thus appears that the inexchangeable Na is located almost entirely in the cytoplasm and hardly at all in the nucleus, and is not bound to macromolecules within the cell.


An evaluation of combined routes of antibiotic administration in vascular reconstructive surgery. A study of local plus parenteral administration of cephaloridine in dogs.


Biosynthesis of prostaglandin E 2  in human skin: subcellular localization and inhibition by unsaturated fatty acids and anti-inflammatory drugs. The biosynthesis of prostaglandin E(2) (PGE(2)) from [1-(14)C]arachidonic acid has been demonstrated in homogenates and subcellular fractions of human epidermis. This biosynthetic capacity is localized in the microsomal fraction, indicating the presence of an active prostaglandin synthetase system associated with membranes of the skin. The incorporation of (14)C from [1-(14)C]arachidonic acid into PGE(2) by the microsomal fraction was enhanced by EDTA. This apparent increase in (14)C incorporation into PGE(2) in the presence of EDTA could be due at least in part to its chelating properties of removing the divalent cations in the homogenate that enhance the selective formation of PGF(2alpha) and the suppression of the activity of epidermal phospholipase A, which causes the release of nonradioactive fatty acid precursors from endogenous phospholipids. This study has also demonstrated that the formation of PGE(2) from arachidonic acid by the microsomal fraction from human skin could be inhibited by polyunsaturated fatty acids, suggesting a possible regulatory role of fatty acids released from endogenous phospholipids on prostaglandin synthesis in this tissue. The inhibitory effects of some anti-inflammatory drugs on skin microsomal prostaglandin synthetase were also demonstrated in these studies. Results from these studies indicate that the skin is therefore a useful tissue for the study of mechanisms of prostaglandin biosynthesis and the mode of action of various anti-inflammatory drugs.


Asthma in childhood.

Asthma in childhood.


Diabetic ketoacidosis in children.

Diabetic ketoacidosis in children.


The aminoacidopathies.

The aminoacidopathies.


Enzyme blocking in neuro-ophthalmology as tools and diseases.


The role of immune reactions in viral encephalitis.


Nystagmographical studies in Aland eye disease.


A unifying concept of idiopathic epilepsy.


The use of animal models for the study of aminoacidopathies.


Contraction of isolated smooth-muscle cells--structural changes. The contraction of isolated smooth-muscle cells has been correlated with evagination of the cell membrane, a marked change in myofilament orientation, and a decrease in cellular volume. Both localized and full contractions have been elicited in the same cell by varying the intensity of electrical stimulation. These results may be explained by a model of the smooth-muscle cell in which the contractile apparatus extends between attachment sites on the cell membrane that are relatively closely spaced.


A protein that binds specifically to the M-line of skeletal muscle is identified as the muscle form of creatine kinase. Published information on the properties of two proteins from chicken muscle, creatine kinase (MM-creatine kinase) and an M-line protein, suggested that they might be identical molecules. Different published procedures were used to purify the two proteins to homogeneity, and the properties of the two preparations were compared. Creatine kinase specific activity increased during purification of M-line protein, reaching a value comparable to that of purified MM-creatine kinase. The two proteins migrated identically in two electrophoretic systems and, after electrophoresis, both could be stained for creatine kinase activity. Double immunodiffusion tests with antibody prepared against MM-creatine kinase established the serological identity of the two protein preparations. Immunofluorescent studies showed that antiserum against MM-creatine kinase was bound in a regular pattern at the centers of the A-band regions of isolated myofibrils. These data show conclusively that the M-line protein and MM-creatine kinase are identical.


Preservation of normal behavior by enucleated cells in culture. BHK-21 fibroblasts and BSC-1 epithelial cells were enucleated with cytochalasin B. The enucleated cells were trypsinized and replated; their subsequent behavior was monitored by light optical techniques. The results demonstrate that the information necessary for attachment, spreading, shape formation, pinocytosis, contact inhibition, and cell locomotion is present in enucleated cytoplasm.


Repeated DNA sequences in the heterochromatic Y chromosome of adult Drosophila melanogaster. DNA isolated from Drosophila melanogaster adults (XX, XY, XXY, XYY) was used in DNA-DNA hybridization experiments on nitrocellulose filters. Filter-bound DNA of "high-heterochromatin" flies (those with one or more Y chromosomes) is more effective in forming hybrid duplexes than is XX DNA. The quantitative difference in hybridization efficiency is due primarily to molecules with a relatively high thermal stability (duplexes that dissociate in the temperature range 80-90 degrees ). Hybridization experiments with DNA samples that have been fractionated into reiterated and unique portions show that the majority of the hybrid duplexes formed involve reiterated DNA. A small, but highly specific, interaction of unique DNA sequences has also been detected in our experiments. These data indicate that a class of repeated DNA sequences is associated with the constitutive heterochromatin, specifically with the Y chromosome of D. melanogaster. Evidence is presented that this same class of molecules, or very similar ones, is to be found in other portions of the genome, presumably in X-chromosomal or centromeric heterochromatin.


Hydrophobic chromatography: use for purification of glycogen synthetase. A homologous series of omega-aminoalkylagaroses [Sepharose-NH(CH(2))(n)NH(2)] that varied in the length of their hydrocarbon side chains was synthesized. This family of agaroses was used for a new type of chromatography, in which retention of proteins is achieved mainly through lipophilic interactions between the hydrocarbon side chains on the agarose and accessible hydrophobic pockets in the protein. When an extract of rabbit muscle was subjected to chromatography on these modified agaroses, the columns with short "arms" (n = 2 and n = 3) excluded glycogen synthetase (EC 2.4.1.11), but the enzyme was retained on delta-aminobutyl-agarose (n = 4), from which it could be eluted with a linear NaCl gradient. Higher members of this series (e.g., n = 6) bind the synthetase so tightly that it can be eluted only in a denatured form. A column of delta-aminobutyl-agarose, which retained the synthetase, excluded glycogen phosphorylase (EC 2.4.1.1), which in this column series and under the same conditions requires side chains 5-(or 6)-carbon-atoms long for retention. Therefore, it is possible to isolate glycogen synthetase by passage of muscle extract through delta-aminobutyl-agarose, then to extract phosphorylase by subjecting the excluded proteins to chromatography on omega-aminohexyl-agarose (n = 6). On a preparative scale, the synthetase (I form) was purified 25- to 50-fold in one step. This paper describes some basic features and potential uses of hydrophobic chromatography. The relevance of the results presented here to the design and use of affinity chromatography columns is discussed.


Inactive enzyme molecules in aging mice: liver aldolase. Evidence is presented that there is a considerable accumulation of inactive fructose-1,6-diphosphate aldolase (EC 4.1.2.7) in the liver of senescent mice. Liver aldolase was purified from 3-month-old mice and used to immunize rabbits. It was demonstrated with the monospecific antibody thus produced that the liver aldolase of young adult (3 month) and aged (31 month) mice are antigenically identical. With the antibody, inactive enzyme molecules (crossreacting material) in liver homogenate of old mice were detected. The liver aldolase of senescent mice had half as much active enzyme per mg of protein, as well as per antigenic unit, as did the liver aldolase of young adult mice. The accumulation of faulty enzyme molecules may be one of the causes of debilitation leading to senescence and death.


Functional correspondence between 30S ribosomal proteins of Escherichia coli and Bacillus stearothermophilus. 30S ribosomal proteins from Bacillus stearothermophilus (B. proteins) have been fractionated and characterized with respect to their ability to replace various E. coli 30S proteins (E. proteins) in the E. coli 30S ribosome reconstitution system. The functional counterparts of all the E. proteins, except S1, S6, S9, and S13, have been tested. In all cases, B. proteins can substitute for E. proteins. Several purified B. proteins are chemically different from their functionally homologous E. proteins. Five B. proteins are immunochemically related to E. proteins; this set includes two proteins that could not be tested in the reconstitution system (S9 and S13). Thus most, if not all, of the E. proteins have functionally equivalent counterparts among B. proteins, even though properties of the two ribosomes are different in several respects. These results suggest that the fundamental structural organization of ribosomes may be the same throughout prokaryotic organisms.


Occurrence of specific pollutants in fish in the Forth and Tay estuaries.


Algal metabolism and water pollution in the Tay region.


cis-Dichlorodiammineplatinum(II) (NSC-119 875): preclinical toxicologic evaluation of intravenous injection in dogs, monkeys and mice.


Relationship of the structure of mescaline and seven analogs to toxicity and behavior in five species of laboratory animals.


Depression of bone marrow and thrombocytopenia associated with chrysotherapy.


Multiple DNA polymerases displayed by isoelectric focusing.


Rapid, direct tissue culture test for toxigenicity of Corynebacterium diphtheriae. A method for testing toxigenicity of Corynebacterium diphtheriae in tissue culture is described. The technique, called the colony overlay test (COT), involves inoculating material from an isolated colony of C. diphtheriae to a small area on the surface of an agar medium which overlays a monolayer of toxin-susceptible HeLa cells. If toxin is produced during incubation at 37 C, it diffuses to the tissue monolayer and destroys the cells below the inoculation site. Twenty-four hours after inoculation, organisms are killed and tissue cells are fixed with formaldehyde. The agar overlay is then removed, and the monolayer is stained with crystal violet. Toxin-affected areas fail to stain or stain poorly. A second plate with antitoxin incorporated in the overlay serves as a control for specificity. Forty-eight strains of C. diphtheriae were tested by the COT, guinea pig, and in vitro, gel diffusion tests. The COT is as specific as the other two tests, is easy to read, and can be used to test large numbers of isolates for toxin action more conveniently than by animal inoculation.


Production of staphylococcal enterotoxins A, B, and C in colloidal dispersions. Larger amounts of enterotoxin were produced when Staphylococcus aureus S-6 was grown under still (nonshaken) conditions in a medium that was a paste or gel than were produced in a liquid dispersion with the same colloidal ingredient or in control basal broth (4% NZ Amine-NAK containing 50 mug of thiamine per 100 ml and 1 mg of niacin per 100 ml). Four colloidal ingredients were used which had been previously demonstrated to not support enterotoxin production in buffer. The effect of the type of dispersion occurred earlier than that of the colloidal ingredient, but interactions were found. This effect was not observed when the cells were grown with aeration (shaken). Four other strains of S. aureus followed a similar pattern for enterotoxins A, B, and C, although liquid and paste with cornstarch and carrageenan were the only media compared to the control broth. Enterotoxins A and B were produced earlier by S. aureus S-6, and much greater quantities of enterotoxins were produced for all strains when incubated shaken.


Differential activity of homologous chromosome loci in ontogenesis.


The effects of certain inorganic ions on in vitro porphyrin--heme formation.


Surface lipid of cells of Pseudomonas aeruginosa and its relation to gentamicin-resistance.


A comparison between immunochemical and physical chemical analyses of the molecular size of urinary proteins.


Extrageniculostriate vision in the monkey. IV. Critical structures for light vs. no-light discrimination.


The acetylcholinesterase of Bungarus fasciatus venom.


Immunological studies on globular and elongated forms of electric eel acetylcholinesterase. Effects of hydrolytic enzymes.


Thyrotrophin secretion from rat pituitary autografts infused with synthetic thyrotrophin releasing factor.


Pyourachus: an unusual surgical problem.


A new method of fixation of silicone rubber catheters for long-term hyperalimentation.


Adjuvants and the reticuloendothelial system. The recall phenomenon of the stimulatory effects of adjuvants with homologous antigen.


Mouse IgM antibodies. I. Quantitation of mouse anti-sheep erythrocyte IgM by absorption and radial immunodiffusion.


Mouse IgM antibodies. II. Variation in mouse specific sheep erythrocyte antibody and total IgM.


Still disease in adults. A cause of prolonged undiagnosed fever.


Treatment of pelvic inflammatory disease in an urban emergency service.


Epilepsy and contact sports. Factors contraindicating participation.


Protein calorie malnutrition and intellectual competence.


Oral care in radiation therapy.

Oral care in radiation therapy.


A procedure for chronic microelectrode recording from cerebellar cortex in the awake cat and monkey.


Management of gastrointestinal fistulas with parenteral hyperalimentation.


Comparative effects of dose protraction and residual injury in dogs and monkeys.


Symptomatic prognosis in patients with idiopathic hypertrophic subaortic stenosis (IHSS).


Identification of ureteral ligation during gynecologic operations.


Studies on uterine flushings in the baboon. I. Method of collection, cellular composition, and protein electrophoretic profiles in animals with and without intrauterine contraceptive devices. Uterine fluid was collected from 11 mature baboons, probably parous, using a "Barbaro" device, developed for sampling human endometrial cells.  Disc electrophoresis was carried out on properly prepared concentrated supernatant fluid.  The electrophoretic patterns did not vary markedly between animals or after fitting of an IUD.  The most marked differences from plasma patterns were in the middle bands (Bands 8-12).  Cytological examination of the precipitate showed the presence of elongated epithelial cells resembling those lining the uterine lumen, cells similar to endometrial stromal cells, large squamous cells resembling cornified vaginal cells,  polymorphonuclearleukocytes, which increased as a response to the insertion of an IUD, mononuclear cells and occasional erythrocytes.


Immunoglobulin levels and liver function tests in normal and toxemic pregnancies.


The pathogenesis of placental infarction. II. An experimental study in the rhesus monkey placenta.


An experimental model of pulmonary arterial granulomatous inflammation. An experimental method to investigate pulmonary granuloma formation in the guinea pig was established. Animals sensitized to human serum albumin (HSA) and challenged intravenously with HSA covalently linked to Sepharose 2B beads developed a specific granulomatous response. This intense pulmonary arterial, focally necrotizing, but mainly granulomatous inflammatory reaction developed 5 to 7 days after the administration of the HSA-bead conjugate. Unsensitized animals did not show such extensive inflammation, but rather exhibited a typical foreign body reaction to the bead. In both the experimental and control groups, the inflammatory response was indistinguishable at 14 and 21 days. Thus the initial acute inflammatory reaction was seen only in specifically sensitized animals. On the other hand, HSA alone produced no demonstrable inflammatory lesion. These observations suggest that locally retained antigen may trigger antigen-specific T (thymus-dependent) lymphocytes to release mediators which contribute to granuloma formation.


Pseudomonas aeruginosa allergy and humoral antibody-mediated hypersensitivity pneumonia.


Scientific Assembly statement. The use of skin tests and serologic tests in histoplasmosis, coccidioidomycosis, and blastomycosis, 1973.


Coronary artery bypass graft haemodynamics.


Direct determination of red cell bound antibody specificity.


The effect of feeding  -2-thienylalanine on phenylalanine metabolism in the rhesus monkey.


Qualitative and quantitative studies on IgG2 globulins in individual human sera with an antiserum capable of differentiating between Gm(n+) and Gm(n-) proteins.


Rapid fixation of the left ventricle: continuous angiographic and dynamic recordings.


Synthesis of actinomycin D lactam, [1',1'-bis(L-threo- , -diaminobutyric acid)]actinomycin D.


Competitive binding to multiple stereospecific binding agents as a means of verifying the identity of a ligand application to cortisol in human umbilical cord blood.


Transmissible mink encephalopathy in carnivores: clinical, light and electron microscopic studies in raccons, skunks and ferrets.


Generalized plane xanthomata with macroglobulinaemia.


Reiter's disease and psoriasis.

Reiter's disease and psoriasis.


An automated system for the regulation and medical review of long-term care facilities and patients.


Preparation of L-tyrosine-ring- 14 C, L-DOPA-ring- 14 C and related metabolites.


Gas-liguid chromatography of parathion metabolites as a sensitive index of induction of liver microsomal mixed-function oxidases.


Estimation of eater-extractable Ca in chicken breast muscle by atomic absorption.


Central effects of sympathomimetic amines.


An assay for the endonucleolytic clevage of RNA to large oligonucleotides.


Determination of the Krebs cycle related keto acids of microorganisms by capillary gas chromatography on glass columns.


Fine structural changes in the placental membrane of the marmoset with increasing gestational age.


Immunologic analysis of noncollagen proteins of human skin.


Antibacterial action of complexing compounds. The influence of disodium versenate on microorganisms in vitro.


Local antigenic stimulation of guinea-pig mammary gland.


The distribution of blood, serum protein and enzyme groups in a series of Lebanese in Australia.


Mechanisms of transfer on immunoglobulins into mammary secretion of ewes.


Comparisons of immunoglobulin secretion in the salivary and mammary glands of sheep.


The diagnosis of tuberculosis in cattle with special reference to bovine PPD tuberculin.


The metabolism of ethyl 4-(3,4,5-Trimethoxycinnamoyl) piperazinyl acetate in rats and dogs.


Modification by sodium salicylate and paraaminosalyicylic acid of the protein binding and antimycobacterial effect of isoniazid in vitro.


The pancreatic  -cell recognition of insulin secretagogues. IV. Islet uptake of sulfonylureas.


Serum autoantibodies and immunoglobulins in hepatitis-associated antigen (HAA)-positive and -negative liver disease. Serum immunoglobulin G, A, and M and serum antinuclear, mitochondrial, and smooth muscle antibody have been measured in 223 patients with hepatitis-associated antigen (HAA)-positive and -negative acute and chronic liver disease. In patients with acute hepatitis, chronic persistent hepatitis, and primary liver cell carcinoma, these indices failed to show any significant differences. However, in the group with chronic aggressive hepatitis the patients who were HAA negative had significantly higher levels of serum IgG, much higher titres of smooth muscle antibody, and often antinuclear and mitochondrial antibodies which were not found in the HAA-positive patients from this group. This suggests that different pathogenic mechanisms may be operative in HAA-positive and -negative chronic aggressive hepatitis.


Isolation of Rickettsia rickettsi in primary bone marrow cell and circulating monocyte cultures derived from experimentally infected guinea pigs. Rickettsia rickettsi was isolated and propagated in primary cell cultures derived from experimentally infected guinea pigs. Organisms were recognized as early as 3 days after cultures were initiated.


Gel filtration studies on immunoreactive gastrin in serum from Zollinger-Ellison patients. Sera from 15 patients with the Zollinger-Ellison syndrome were subjected to gel filtration on Sephadex G-50 superfine columns (10 x 2000 mm). The concentration of gastrin in the effluent was determined by a sensitive radioimmunoassay. Immunoreactive gastrin was eluted in four components in 14 sera. (1) Component I, eluted in the same position as proinsulin, constituted 9.7 +/- 1.2 (mean +/- SEM)% of the total immunoreactivity. (2) Component II (;big gastrin') eluted between proinsulin and insulin constituted 57.8 +/- 4.1% (mean +/- SEM) of immunoreactive gastrin. In three sera with the highest concentration of gastrin, component II appeared biphasic. (3) Component III (;little gastrin') was distributed in two peaks; the first one eluted in the same position as the heptadecapeptide gastrin II made up 17.4 +/- 2.7 (mean +/- SEM)% of the total immunoreactivity; the second one eluted in the same position as gastrin I constituted 9.5 +/- 1.3 (mean +/- SEM)%. (4) Component IV (;minigastrin') was eluted immediately before the salt peak and constituted 5.6 +/- 1.4 (mean +/- SEM)%. In one serum only components I and II were present. After incubation with trypsin all immunoreactivity in components I and II was converted to heptadecapeptide-like gastrins.The findings suggest that immunoreactive gastrin in serum from Zollinger-Ellison patients is circulating in at least four components of different molecular size.


The pharmaceutical market and prescription drugs in the Federal Republic of Germany: cross-national comparisons.


Multiplicity of Scrapie virus in infected mouse spleen cells in vivo. Subpopulations of spleen cells from scrapie virus-infected mice were used to determine the average virus content of infected cells in vivo at a time when virus was rapidly increasing in titer in lymphoreticular tissues. Comparison of the mean lethal doses of lysed to intact cells indicated averages of 2 to 6 infectious units per infected cell. In another experiment, preparations of cytoplasmic nucleic acids extracted from spleen cells of infected mice had no detectable infectivity, which suggests that the transmissible form of the virus is not a free nucleic acid.


Immunologic response of man to group B meningococcal polysaccharide vaccines.


A method for measurement of antibiotics in human interstitial fluid.


Shigellosis due to Shigella dysenteriae. 1. Relative importance of mucosal invasion versus toxin production in pathogenesis.


Effect of rifampicin on delayed-hypersensitivity reactions.


Adherence of Neisseria gonorrhoeae to urethral mucosal cells: an electron-microscopic study of human gonorrhea.


Treatment of funnel-web spider bite.

Treatment of funnel-web spider bite.


Mutagenic DNA polymerase in B. subtilis.


Quantitating cholinergic receptor molecules.


Laboratory diagnosis of multiple sclerosis. Elevation of immunoglobulin G in cerebrospinal fluid.


Diagnosis of meningococcal arthritis by immunoelectrophoresis of synovial fluid. In a case of meningococcal arthritis, the organism was identified promptly through examination of synovial fluid by countercurrent immunoelectrophoresis.


Preparation of solid-phase immunosorbents by coupling human serum proteins to cyanogen bromide-activated agarose. The method of preparing solid-phase immunosorbents by covalently attaching proteins from whole human serum to cyanogen bromide-activated agarose has been investigated to determine optimum concentrations of cyanogen bromide and protein, and the optimum pH for the maximum attachment of proteins from serum. Two systems in which the above immunosorbents have proved useful are described: the removal of antibodies to normal serum proteins from anti-hepatitis B serum and the removal of light chain antibodies from anti-human immunoglobulin M serum.


Aflatoxin production of species and strains of the Aspergillus flavus group isolated from field crops. Peanuts, cottonseed, rice, and sorghum from Texas were sampled over a 3-year period. To insure adequate isolation of alfatoxin-producing species of fungi, low-quality lots were sampled at a rate greater than their respective proportional representation. Aflatoxins were found each year in peanut and cottonseed and were found in 2 of 3 years in rice and sorghum. Aflatoxins were detected in all four crops. The Aspergillus flavus group was much more prevalent in peanut and rice than in cottonseed and sorghum. Of the isolates of the A. flavus group, 96% from peanuts, 79% from cottonseed, 49% from sorghum, and 35% from rice produced aflatoxins. The average toxin production of isolates from rice was much less than that from peanuts, cottonseed, or sorghum. More than 90% of all isolates of the A. flavus group were identified as the species A. flavus. A. parasiticus was isolated from all four crops. Only A. parasiticus produced aflatoxin G.


Control of surface wound infection: skin versus synthetic grafts. Auto-, iso-, or xenografts of skin and synthetics placed on surface wounds freshly contaminated with Pseudomonas aeruginosa stabilizes the wound bacterial population in rats over a 24-h period. When these wounds contained a bacterial contamination established for 24 h prior to grafting, only skin and the synthetic polyhydroxyethylmethacrylate were effective in lowering the initial bacterial concentration. Polyurethane foam and nylon velour were not effective in the established infection model. Skin placed on a contaminated wound for 2 h or longer appeared to equilibrate with the underlying muscle so that the bacterial count per milligram of skin was similar to that of the muscle. It was suggested that this preparation would be useful to obtain an estimate of surface contamination without biopsy of the infected muscle. Skin grafts in place for 2 h significantly lowered the bacterial count in a wound with an established infection. A second decrease occurred between 4 and 24 h after grafting. Histological studies of contaminated and exposed panniculus muscle showed that leukocytes tend to migrate from the muscle surface to its base. Skin grafts and polyhydroxyethylmethacrylate appear to reverse the white cell migration so that the cells move toward the surface of the muscle with preservation of normal staining characteristics in the muscle. It is suggested that this alteration in cell movement after graft application might modify the white cell function and result in a greater bactericidal activity. Apparently, grafts lower bacterial levels in an established infection by modifying the host response to the surface contamination.


Culture of Ethiopian strains of Borrelia recurrentis. A number of standard bacteriological media with supplements were tested for their ability to support in vitro growth of Ethiopian strains of Borrelia recurrentis. Propagation of 18 out of 21 strains occurred in Trypticase soy yeast broth to which bovine albumin (fraction V), N-acetyl glucosamine, and sodium pyruvate had been added. This medium supported a population of 10(7) organisms per ml and yielded a harvest of four to five times the original inoculum during the logarithmic phase of growth. Maximal yield varied from 1.4 x 10(7) to 3.4 x 10(7) organisms per ml. Generation time in optimal media was 11.3 h. Lesser multiplication of organisms occurred in other media tested. Strains from primary cultures were infective for the green monkey. Recovery of viable organisms from subculture has not been successful.


Indirect fluorescent-antibody method for the identification of Corynebacterium vaginale. The indirect fluorescent-antibody technique was employed in an attempt to develop a rapid method of identification of Corynebacterium vaginale. Six reference strains and ten clinical isolates selected on the basis of morphology and conventional biochemical tests were compared. Antisera were prepared in rabbits against the six reference strains. The most satisfactory antiserum was that prepared using strain 14018 grown diphasically (14018 Di) as the antigen. Certain of the antisera did exhibit a cross-reacting titer when reacted against Corynebacterium diptheriae, Corynebacterium xerosis, or Lactobacillus acidophilus. However, antisera adsorbed with these bacteria did not exhibit a significant decrease in titer when reacted against the homologous strain. Various other species of Corynebacterium as well as species of Nocardia, Actinomyces, Hemophilus, and Streptococcus did not fluoresce with the antisera. A specific antiserum was prepared by adsorbing anti-14018 Di with L. acidophilus. The adsorption removed the cross-reacting antibody but did not affect the staining reaction with C. vaginale strains. All reference strains and clinical isolates characterized as C. vaginale gave a definite positive reaction with the adsorbed anti-14018 Di. The specificity of the reactions was assessed by adsorbing the antiserum with the homologous strain. The data suggest that the indirect staining method will be of value in the rapid presumptive identification of C. vaginale.


Cicatricial pemphigoid. Immunofluorescence investigations.


Preservation of mycobacteria: 100 percent viability of suspensions stored at -70 C. Our earlier studies on long-term preservation of mycobacteria have been expanded to include other species in this genus. Mycobacterium kansasii and M. marinum, like mammalian tubercle bacilli and BCG, survive much better when stored at -70 C. By statistical analysis, M. gordonae, M. scrofulaceum, M. xenopi, M. avium, M. intracellulare, M. terrae, M. fortuitum, and M. smegmatis survived equally well at -20 C or -70 C; however, viable counts of all strains stored at -20 C were always lower than those of paired suspensions stored at -70 C, suggesting that the lower temperature is preferable for prolonged storage periods. Advantages of preservation at -70 C in Tween-albumin liquid medium are: (i) 100% viability of bacterial populations for long periods, (ii) highly reproducible inocula for animal experiments, (iii) minimal clonal selection of undesirable mutants, (iv) maintenance of genetic characteristics, and (v) adaptability to a "seed lot" system. On the basis of available information, a discussion of lyophilization versus freezer storage is presented.


Gold sodium thiomalate treatment of pemphigus.


The middle temporal visual area(MT)in the bushbaby, Galago senegalensis.


Acute myelomonocytic leukemia with macroglobulinemia, Bence Jones proteinuria, and hypercalcemia.


The role of iron in bacterial infections, with special consideration of host-tubercle bacillus interaction.


Effects of methylmercuric chloride on the progeny of mice and rats treated before or during gestation.


Metabolism and elimination of sulphite by rats, mice and nonkeys.


More meditations of MSG.

More meditations of MSG.


Applications of a simplified triple-test cross.


Asymptomatic ossification of the posterior longitudinal ligament of the cervical spine. Incidence and roentgenographic findings.


Experimental autoimmune damage to rabbit male accesory glands.


Anti-nociceptive activity of clonixin in rhesus monkeys.


Electron microscopic and serological studies on simian virus S.A. 11 and the "related" O agent.


Neurophysiology of color vision.

Neurophysiology of color vision.


Metabolic characteristics of  241 Am in the adult baboon.


Carbonic anhydrase quantity of red blood cells in morbus basedowii.


Bile reflux gastritis. Diagnosis, medical and surgical therapy.


Studies on prephenoloxidase-activating enzyme from cuticle of the silkworm Bombyx mori. I. Activation reaction by the enzyme.


Studies on prephenoloxidase-activating enzyme from cuticle of the silkworm Bombyx mori. II. Purification and characterization of the enzyme.


Preparation of rubella virus antigens for immunodiffusion by mild extraction of infected cells with a neutral detergent.


Immunochemical studies of Sindbis virus variants.


An absolute requirement for insulin in the control of hepatic glycogenesis by glucose.


Kinetic parameters of human carbonic anhydrase B as determined from NMR linewidths of  13 C in CO 2  and HCO 3  - .


Sulfhydryl reactivity of organic nitrates: biochemical basis for inhibition of glyceraldehyde-P dehydrogenase and monoamine oxidase.


Metabolism of  35 S-methionine and  35 S-cystine in the pregnant rhesus monkey.


The management of carcinoma of the breast by radical supervoltage radiation.


Non-pulmonary surgical tuberculosis.

Non-pulmonary surgical tuberculosis.


Chromatography of Drosophila tRNA on BD-cellulose.


The immune response to thetaX 174 in man. I. Primary and secondary antibody production in normal adults.


A comparison of in vitro, immunosuppressive and clinical effects of goat and horse anti-human thymocyte globulin in Macaca monkeys.


Self-inflicted rotation osteotomy of the tibia.


Stereotaxic treatment of frontal-lobe and centrencephalic epilepsy.


Immunoglobulin molecules on the surface of activated T lymphocytes in the rat. Lewis strain rat lymphocytes were exposed in vitro to a variety of specific and nonspecific blastogenic stimuli. The surfaces of the transformed lymphocytes were examined by indirect immunofluorescence for the presence of T cell antigens and immunoglobulin molecules. More than 90% of lymphocytes that underwent blast transformation after exposure to foreign histocompatibility antigens (mixed lymphocyte reaction; in vitro allograft reaction), purified tuberculin, phytohemagglutinin (PHA), and concanavalin A (Con-A) had T cell antigenic markers on their surfaces. 70-92% of the antigen-stimulated blast cells also had readily detectable surface immunoglobulin molecules, whereas less than 3% of the PHA- and Con-A-activated cells were Ig(+). Pokeweed mitogen (PWM) appeared to activate both B and T cells, but the T cells did not have detectable surface immunoglobulin molecules. Nonactivated control cultures contained T(+)Ig(-) lymphocytes almost exclusively. The results suggest that thymus-dependent rat lymphocytes express increased amounts of detectable immunoglobulin on their surface in response to specific stimulation with antigen. It is postulated that the acquisition of immunological competence by activated T cells may be related to this expression of surface immunoglobulin.


Value of normothermic perfusion, hypothermic perfusion, and durotomy in the treatment of experimental acute spinal cord trauma.