Immunogenicity of purified, inactivated chikungunya virus in monkeys. Chikungunya (CHIK) virus, harvested from infected BHK-21 cell cultures and highly purified by a method combining zinc acetate precipitation, Sephadex-Sepharose column chromatography, and sucrose density gradient centrifugation, was subjected to ultraviolet (UV) irradiation and treatment with formalin. Inactivation of the virus by UV light was apparently a first-order reaction. The virus treated with 0.05% formalin at 4 degrees C was inactivated completely after 7 weeks, but when treated with 0.025% formalin retained its plaque infectivity at least 17 weeks. Both UV- and formalin-inactivated viruses induced production of anti-CHIK neutralizing antibodies in Japanese monkeys with no preimmune antibodies. Judging by the titres of antibodies produced, the immunogenic effect of the UV-irradiated virus was superior to that of the formalin-treated virus.


A search for the epidemic typhus agent in Ethiopian ticks. The presence of antibodies to Rickettsia prowazeki in domestic animals from several parts of Africa, and the isolation of this rickettsia from the blood of goats and sheep and from ticks off cattle or camels in Ethiopia, led to the hypothesis that R. prowazeki in nature may occur in an extrahuman cycle involving ticks and domestic animals. This study attempted to recover R. prowazeki from 2 624 ticks (4 genera, 10 species) collected in central and southern Ethiopia. The ticks were examined by the haemolymph test and by the injection of tissues into guineapigs. No strains of typhus rickettsia were received and there was no serologic evidence suggesting the presence of this agent in any of the ticks examined. One Amblyomma cohaerens contained an organism that reacted specifically with fluorescing antibodies against R. prowazeki; attempts to isolate and identify this agent failed. Fifty-seven (2.2%) Amblyomma ticks (26 A. gemma, 17 A. variegatum, 14 A. cohaerens) were infected with rickettsiae of the spotted fever group, and probably represented R. conori or closely related rickettsial agents.


Vaccination of Rhesus monkeys (Macaca mulatta) against Plasmodium knowlesi by the use of nonviable antigen. This study was part of a long-term project with the ultimate goal of developing a malaria vaccine for use in man. Rhesus monkeys that had been vaccinated with a nonviable antigen given in combination with Freund's adjuvant were protected against a normally lethal challenge of Plasmodium knowlesi. Use of the antigen alone or in combination with other adjuvants was not successful. The fact that monkeys were protected against an infection that is normally lethal suggests that a similarly prepared antigen might be of use against malaria in human beings.


Studies of folic acid compounds in nature. 3. Folic acid compounds in cabbage.


Effect of amino acid starvation on UV sensitivity of Lactobacillus acidophilus cells.


Immunochemical studies on mannans of the genus Saccharomyces. Group of Torulaspora species.


Quantitative determinations of complement components and immunoglobulins in tears and aqueous humor.


Regulation of biosynthesis of secondary metabolites. XV. Isolation and characterization of auxotrophic mutants in Streptomyces aureofaciens.


The nerve-fiber layer of the primate retina: an autoradiographic study.


Report on drugs, acidic and neutral nitrogenous.


Report on multiresidue methods (interlaboratory studies).


Reverse phase thin layer chromatography of some aroclors, halowaxes, and pesticides.


Ceramic-coated subperiosteal implants. II. Clinical and histologic evaluations.


The activation of mononuclear phagocytes in vitro: immunologically mediated enhancement.


Neurogenic influence on pulmonary compliance.


Pharmacologic increases in erythrocyte 2,3-diphosphoglycerate for therapeutic benefit.


Direct determination of carbaryl by gas-liquid chromatography using electron capture detection.


Photoreduction of 4,4'-dichlorobiphenyl.


The relationship of NSD to reactions and complications following treatment for malignant uterine cervical neoplasms.


Periosteal new bone formation (periosteal neostosis) in renal osteodystrophy. Relationship to osteosclerosis, osteitis fibrosa, and osteoid excess.


Australia antigen in patients with viral hepatitis.


Rickettsioses in Dalmatia (Yugoslavia).

Rickettsioses in Dalmatia (Yugoslavia).


Criteria for an animal model of alcoholism.


Sciatic nerve entrapment by ectopic bone after posterior fracture-dislocation of the hip.


On T-cell-mediated cytotoxicity.

On T-cell-mediated cytotoxicity.


New genetic model for allelism at histocompatibility and other complex loci: polymorphism for control of gene expression.


Heart rate and respiratory rate changes during drug-induced impairment in a conditioned avoidance task in monkeys.


A review of methods to induce alcohol addiction in animals.


Human uromucoid. I. Quantitative immunoassay.


Functional units of Streptomyces hyphae, equivalent with prokaryotic cells.


A comparison of d-amphetamine, l-amphetamine, and methamphetamine self-administration in rhesus monkeys.


Evidence for ascending noradrenergic mediation of hypothalamic hyperphagia.


Experimental techniques for testing the sensitivity of bladder tumours to antineoplastic drugs.


A simple passive hemagglutination-inhibition technic for determination of fibrin degradation products and fibrinogen.


Another example of anti-D in a D-positive patient.


Isolation of Neisseria meningitidis from the vagina and cervix.


Aspergillus myocarditis.

Aspergillus myocarditis.


Heritability estimates and genetic and environmental correlations for the human immunoglobulins G, M, and A.


Blood groups and immunoglobulin groups in Trio and Wajana Indians from Surinam.


Position of the Duffy locus on chromosome 1 in relation to breakpoints for structural rearrangements.


Evolving health delivery systems and older people.


The fastest growing minority: the aging.


Health services: their success and failure in reaching older adults.


Health status of older people: cross-national implications.


Rifampin in initial treatment of pulmonary tuberculosis. A.U.S. Public Health Service tuberculosis therapy trial.


Bacteriocin-typing of Mycobacterium tuberculosis.


Osteomyelitis caused by concurrent infection with Mycobacterium tuberculosis and Blastomyces dermatitidis.


Thyroxine transport and metabolism in methadone and heroin addicts.


The plasminogen activator of vampire bat saliva.


The relationship between the urethral flora and urinary infection in the catheterised male.


Effect of phenobarbitone on plasma (14C)bilirubin clearance in patients with unconjugated hyperbilirubinaemia.


Unsaturated fatty acid synthesis is not required for induction of lactose transport in Escherichia coli.


Purification and properties of an endo-beta-N-acetylglucosaminidase from Streptomyces griseus.


Recombination at the bar locus in an inverted attached-X system in Drosophila melanogaster. Recombination at the Bar locus in Drosophila melanogaster was investigated in an inverted attached-X system which enhanced the frequency of homozygosis for the Bar region. Females among the progeny of homozygous B mothers were searched for changes of B to BB and to B(+). Marker genes were followed and exceptional half-tetrads were analyzed in regard to two hypotheses: that of exchange between obliquely synapsed members of the duplication, which is associated with exchange of outside markers, and that of intrachromosomal exchange, which does not involve recombination of markers.-Recombinant exceptions of B(+) /BB genotype, carrying the outside marker combinations predicted on the hypothesis of exchange between obliquely synapsed duplication members, were encountered repeatedly. It is established that B(+) and BB strands are reciprocal products of the same event.-Twelve nonrecombinant exceptional strands were isolated; ten of these were B(+) and two were BB. Only one of the nonrecombinant half-tetrads offered the opportunity to test the prediction of reciprocity of the intrachromosomal event. Analysis showed the exceptional female to be of the constitution BB/B, a type not expected on the hypothesis. While it could have arisen through some kind of copy error in the repair of a chromatid break, a valid test of the hypothesis of intrachromosomal exchange must rest on the isolation and analysis of more cases of the appropriate exceptional genotype.-In several cases Bar changes were found to be associated with aberrations; all but one of these involved spontaneous, cytologically identifiable deletions.


Heterogeneity of echovirus type 17: comparison of the two lines of the prototype strain.


The release of intact oligosaccharides from specific glycoproteins by endo-beta-N-acetylglucosaminidase H.


The structural gene for O-acetylserine sulfhydrylase A in Salmonella typhimurium. Identity with the trzA locus.


Isolation of a second form of calsequestrin.


Morphology of the inferior olivary complex of the rhesus monkey (Macaca mulatta).


Comparison of the infectivity and pathogenicity of six species of African schistosomes and their hybrids. 2. Baboons.


Isolation and characterization of a blood P1 active carbohydrate antigen of Echinococcus granulosus cyst membrane.


A potent non-peroxidase glycoprotein immunogen in commercial horseradish peroxidase.


Fractionation of sera from operationally tolerant rats by DEAE cellulose chromatography; evidence that serum blocking factors are associated with IgG.


A functional abnormality of the fifth component of complement (C5) from human serum of individuals with a familial opsonic defect.


Rabbit secretory IgA: identification and genetic control of two allotypes of secretory component.


Autoimmune murine thyroiditis. IV. Localization of genetic control of the immune response.


Counterimmunoelectrophoresis with adenovirus type-specific anti-hemagglutinin sera as a rapid diagnostic method.


Differential contributions of spinal pathways to body representation in postcentral gyrus of Macaca mulatta.


Response characteristics of single cells in the monkey superior colliculus following ablation or cooling of visual cortex.


Reconstruction of population response to a vibratory stimulus in quickly adapting mechanoreceptive afferent fiber population innervating glabrous skin of the monkey.


Evidence for an endogenous antigen in the adjuvant arthritic rat.


Adrenal function in normal infants and in marasmus and kwashiorkor: plasma aldosterone concentration and aldosterone secretion rate.


Letter: Wheat farmer's asthma in Australia.


Glucose intolerance in infants of very low birth weight. I. Incidence of hyperglycemia in infants of birth weights 1,100 grams or less.


Total and free thyroid hormone concentrations in the neonatal period.


Custody of the myelodysplastic child: implications for selection for early treatment.


Separation of photosynthetic systems I and II.


7S immunoglobulin M (IgM) from serum purified by anti-mu-chain immunoadsorbent: comparison of the reassociation properties of 7S IgM and various 19S IgM subunits.


Low-molecular-weight antibody in rabbit urine: identity with the Fab immunoglobulin fragment.


Studies on viral hepatitis and hepatitis B (Australia) antigen.


Ecto-enzyme of granulocytes: 5'-nucleotidase. The 5'-nucleotidase of guinea pig polymorphonuclear leukocytes is localized exclusively on the plasma membrane of intact cells. The active site of this enzyme faces the external medium, not the cytoplasm.


Synthesis and expression of surface antigens during the cell cycle.


Development of functional tolerance to ethanol in rhesus monkeys (Macaca mulatta).


B and T cell markers in human proliferative blood diseases and primary immunodeficiencies, with special reference to membrane bound immunoglobulins.


Juvenile xanthogranuloma diagnosed by paracentesis.


Variant angina pectoris caused by coronary artery spasm.


Ocular toxicity from ethambutol.

Ocular toxicity from ethambutol.


Use of chemically defined diets in the management of patients with high output gastrointestinal cutaneous fistulas.


Changing methods in the treatment of severe pancreatitis.


Further studies on nicotine-induced alterations in histamine metabolism in the rat.


The high incidence of anti-HL-A antibodies in anti-D typing reagents. Illustrated by a case of Matuhasi-Ogata phenomenon mimicking a "D with anti-D" situation.


Tonsillectomies: in dollars and cents.

Tonsillectomies: in dollars and cents. Over the last quarter of a century, much controversy has been generated on the indications for tonsillectomy and adenoidectomy. The economic aspects of this operation have received comparatively little attention. The rate per 10,000 children 0 to 19 years of age for the whole province of Ontario for the year 1971 was 174 and ranged from a high of 307 in one county to a low of 59 in another. The total cost of these operations to the health insurance plan was 11.4 million dollars. National studies on the indications for these operations, their benefits and hazards are urgently required.


Metabolic studies on polychlorinated biphenyls. II. Metabolic fate of 2,4,3',4'-tetrachlorobiphenyl in rats.


Metabolic studies on polychlorinated biphenyls. 3. Complete structure and acute toxicity of the metabolites of 2,4,3',4'-tetrachlorobiphenyl.


The influence of dialysis in 2-mercaptoethanol reduction of erythrocyte antibodies.


Hepatitis carriers among soldiers who have returned from Vietnam. Australia antigen studies.


Letter: The cell division of the pigment epithelium in the repairing retina after photocoagulation.


Congenital pseudarthrosis of the radius.


Pseudomonas septicemia in neutropenic dogs. I. Treatment with granulocyte transfusions.


Immune response of pathogen-free mice inoculated intranasally with Mycoplasma pulmonis.


Evaluation of the use of albumin controls in Rh phenotyping.


The association of J chain with the Fc region of human IgM.


Human J-chain: isolation and molecular weight studies. J-chain, a polypeptide chain unique to polymeric immunoglobulins (Igs) which has been postulated to be responsible for joining monomeric subunits into the polymeric forms, was isolated from human IgA, secretory IgA, and IgM by 3 different procedures, disc electrophoresis, immunoadsorbent radioimmunoassay, and dialysis against distilled water.  Precipitation in water was the simplest and yielded satisfactory results.  Molecular weights of the various J-chain isolates were studied by using 2-species plot analysis of sedimentation equilibrium data.  2 populations of molecules were found: 1 had a molecular weight of 13,300-17,700 and the other of 6400-11,500.  Variations in these data from those of other investigators are discussed in terms of isolation procedure differences.


Isolation and characterization of the proenzyme form of the C1s subunit of the first complement component.


Antigenicity of reduced and alkylated Australia antigen.


Membrane receptors and in vitro responsiveness of lymphocytes in human immunodeficiency.


Immunoglobulins in CSF in multiple sclerosis and other neurological disorders.


Letter: Intal in childhood asthma.

Letter: Intal in childhood asthma.


The baboon. Hematology.

The baboon. Hematology.


The baboon. Clinical chemistry.

The baboon. Clinical chemistry.


The baboon. Parasitology.

The baboon. Parasitology.


Immunosuppressive activity of the Pseudomonas aeruginosa endotoxin on a lysozyme anti-lysozyme system.


Phenoloxidase system in wild and mutant adults of drosophila melanogaster. I. Activation and activity of mono- and diphenoloxidases.


Ribonucleoprotein particles containing mRNA and pre-mRNA.


Non-ribosomal polypeptide synthesis.

Non-ribosomal polypeptide synthesis.


Specific secretory protein of the female genital tract.


Protein synthesis in mammary gland.

Protein synthesis in mammary gland.


Monoamine-containing cell bodies in the squirrel monkey brain.


Osteoradionecrosis of the jaw.

Osteoradionecrosis of the jaw.


The influence of oxygen on the fluorescence of lipoxygenase.


Effect of mirex on the activities of various rat hepatic mixed-function oxidases.


The conversion of bisnorbiotin and bisnordethiobiotin to biotin and dethiobiotin, respectively, by microorganisms.


Immunochemistry of sperm-whale myoglobin. XVII. Conformation and immunochemistry of derivatives modified at lysines 98, 140 and 145 by reaction with 3,3-tetramethyleneglutaric anhydride.


Heat denaturation of bovine serum albumin in alkaline pH region.


Conformational change of bovine serum albumin induced by O.IO m Tris-EDTA-boric acid buffer at pH9.


Vitamin B12 binding proteins in monkey serum and red cells.


Snake venom toxins. The amino acid sequences of two toxins from Dendroaspis jamesoni kaimosae (Jameson's mamba) venom.


Compensatory renal hypertrophy in eviscerated rats.


Olfactory recognition in the infant squirrel monkey.


Growth and behavior of surrogate-reared squirrel monkeys.


Non-specific immunologic precipitin reactions in alpha-fetoprotein determination. Analysis of two cases.


Problems in the definition of ill health.


A simple method of preparation of gastrointestinal tract tissues for scanning electron microscopy.


Perinatal immunity in calves.

Perinatal immunity in calves.


The stability of second-order conditioned limb responses established in hens with cerebral electrical stimuli.


Rheumatic fever presenting as an acute abdomen.


Alterations of serum opsonins in patients with severe thermal injury.


Erythroblast ferritin: synthesis, structure, and function in developing erythroid cells.


The kinetics of 67gallium incorporation into inflammatory lesions: experimental and clinical studies.


A new look at mumps encephalitis: inclusion bodies and cytopathic effects.


Diarrhea toxin obtained from a waterbloom-producing species, Microcystis aeruginosA Kützing. A diarrhea-producing toxin from a blue-green alga, Microcystis aeruginosa Kützing, was obtained from standing laboratory cultures. The non-dialyzable fraction of the lysate from whole cells produced fluid accumulation in the ligated small intestinal loops in guinea pigs.


Teratogenic effects of aldrin, dieldrin, and endrin in hamsters and mice.


Teratogenic effects of ochratoxin A in mice.


Critical point drying of protozoan cells and other biological specimens for scanning electron microscopy: apparatus and methods of specimen preparation.


The variant form of angina: diagnostic and therapeutic implications.


Animal reservoirs and Gambian trypanosomiasis.


Nephrogenic diabetes insipidus secondary to declomycin.


Steady-state enzyme kinetics with high-affinity substrates or inhibitors. A statistical treatment of dose-response curves. A statistical treatment of steady-state enzyme kinetic measurements is described that allows for depletion of free substrate or free inhibitor concentrations owing to significant binding to the enzyme. V(max.), K(m) or K(i), enzyme concentration, the concentration of substrate or inhibitor required for a half-maximal effect and standard errors of these parameters can be calculated from dose-response measurements; the concentration of each component of the system may be estimated also. The statistically best values of the parameters are used to convert dose-response curves into convenient linear forms. The method is applied to dose-response measurements of hydroxyquinoline N-oxide inhibition of bacterial respiration and aminopterin inhibition of dihydrofolate reductase. Two FORTRAN programs for this method have been deposited as Supplementary Publication no. SUP 50019 at the National Lending Library for Science and Technology, Boston Spa, Yorks. LS23 7BQ, U.K., from whom copies may be obtained on the terms indicated in Biochem. J. (1973) 131, 5.


Letter: Acute pancreatitis and diabetic ketoacidosis in hypothermia.


Gastrointestinal problems in the old. II.


Specific lymphocyte stimulation by purified, heat-inactivated hepatitis-B antigen. The ability of purified, heat-inactivated hepatitis-B antigen (HBAg) to stimulate sensitized lymphocytes in vitro was investigated with the lymphocyte stimulation test on lymphocytes from three groups of individuals. Stimulation was minimal in the lymphocytes of two out of 15 normal controls, whereas lymphocytes from nine out of 12 patients who had recovered from hepatitis B showed stimulation, as did lymphocytes from five out of 12 laboratory technicians who had been regularly exposed to HBAg but who had no history of hepatitis or signs of it in the previous two years. No differences were observed in the responses to phytohaemagglutinin of lymphocytes from persons in the three groups. HBAg and HBAg inactivated by heat were shown in immunodiffusion to be immunologically identical. Inactivated HBAg stimulated antibody production in guinea-pigs. These findings suggest that not only humoral but also cell-mediated immunity might be induced by vaccination with purified, heat-inactivated HBAg.


Use of Tenckhoff catheter for peritoneal dialysis in terminal renal failure. Over a period of 33 months a total of 2,146 peritoneal dialyses were carried out by means of indwelling Tenckhoff catheters in 65 patients suffering from terminal renal failure. The patients were maintained on peritoneal dialysis for periods varying from two weeks to 13 months. Treatment over long periods was possible in only a few cases. Infection and clotting, which tended to limit the functional life of the catheters, was reduced by rigid asepsis and by adding heparin to the dialysate. The Tenckhoff catheter was found to be valuable for peritoneal dialysis as a short-term measure, especially in patients in whom haemodialysis was not immediately feasible, in borderline cases when kidney function was not too seriously impaired, and as an alternative to haemodialysis when that was interrupted by complications.


Sulfuric acid for the cleanup of animal tissues for analysis of acid-stable chlorinated hydrocarbon residues.


Cancer statistics, 1974--worldwide epidemiology.


Detection of cervical neoplasia: reducing the risk of error. In cytology studies false negative smears range from 1.8% to 20% due to different levels of cytologic expertise and of material presented to the pathologist.  Only 6% of in situ carcinomas of the cervix have an abnormal gross appearance so routine multiple biopsies are needed.  Schiller's test, using a weak aqueous solution of iodine, leaves an area of carcinoma unstained but colors normal cervical epithelium a dark brown.  However, not all areas which fail to stain represent foci of cancer.  Multiple biopsies and endocervical curettage are required, particularly to detect lesions not involving the portio.  Conization is the most accurate method for evaluating the suspicious cervix.  80 histo logic sections should be examined from each cervix specimen.  Complicati ons of conization may be hemorrhage, cervical stenosis, uterine perforation, and pelvic infection.  Only after 4-6 weeks does pelvic infection cease to be a significant risk.  When done during pregnancy, conization may cause a fetal loss of 15%.  Colposcopy has a low false-negative rate but cannot detect lesions inside the cervical canal.  It is the best method of clinical evaluation of patients with abnormal cytological smears, particularly during pregnancy.  A colposcopic diagnosis is based on an evaluation of vascular pattern, intercapillary distance, surface contour, color tone, and clarity of demarcation.  Where colposcopic examination is unsatisfactory and cytology is positive, conization is indicated.  The final diagnosis depends on the histology of the directed biopsy.


Three-dimensional structures of giant mitochondria, dictyosomes and "concentric lamellar bodies" formed during the cell cycle of Euglena gracilis (Z) in synchronous culture.


Removing substances from blood by affinity chromatography. I. Removing bilirubin and other albumin-bound substances from plasma and blood with albumin-conjugated agarose beads. Substances such as bilirubin that bind tightly to plasma proteins cannot readily be removed from blood. We describe here the use of affinity chromatography as a new approach to the removal of proteinbound metabolites and toxins from blood. Agarose beads were coupled via cyanogen bromide to human serum albumin so as to contain 30-50 mg of albumin/g wet wt. Such beads, when exposed to plasma from a patient with congenital nonhemolytic jaundice labeled with [(14)C]-bilirubin, bound more than 150 mug bilirubin/g of beads. The binding was saturable, concentration-dependent, relatively independent of flow rate, and reversible by elution with plasma, albumin, or 50% (vol/vol) ethanol. The beads could be repeatedly reused without loss of efficiency after ethanol elution and long storage in the cold. Salicylate, cortisol, and taurocholate, which bind weakly to albumin, were retarded by the beads but eluted with neutral buffer. Thyroxine, taurolithocholate, chenodeoxycholate, and digitoxin bound tightly but were eluted with 50% ethanol. Digoxin did not bind at all. When whole blood was passed over agarose-albumin beads, bilirubin was removed, calcium and magnesium fell slightly, but red cells, white cells, platelets, clotting factors, and a variety of electrolytes and proteins were substantially unchanged. Agarose-albumin beads may be useful for removing protein-bound substances from the blood of patients with liver failure, intoxication with protein-bound drugs, or specific metabolic deficits. Furthermore, it may be possible to make useful adsorbents by attaching other proteins to agarose or other polymer beads.


Isolation of a novel glycoprotein from the urine of a patient with chronic myelocytic leukemia. Patient B. J. with chronic myelocytic leukemia excreted 0.5-1.1 g protein per day in the urine. Gel filtration on Sephadex G-75 showed about one-third of this protein to be in molecular weight range 20,000-40,000 (fraction BJC). BJC, prepared from 9 liters of urine by gel filtration, was chromatographed on carboxymethylcellulose. Two proteins were eluted from the resin in pure form (as shown by zone and immunoelectrophoresis) in yields representing 8 and 3 mg/liter of urine: BJC1 and BJC2. Their amino acid compositions were identical. BJC1 contained 61% carbohydrate (33% hexose, 11% sialic acid, 13% glucosamine, 5% galactosamine). BJC2 contained one-fourth to one-half as much of each carbohydrate. Molecular weight of BJC1 was estimated at 29,000 by gel filtration. Neither glycoprotein reacted with rabbit antiserum to normal human serum.Antiserum to BJC1 was made in the rabbit. Immunoelectrophoresis with this antiserum showed a faint precipitin line, corresponding in mobility to BJC1, in normal human plasma, and a stronger line in most leukemic plasmas. By immunodiffusion, BJC1 was not detectable in normal human urine, but a positive reaction occurred in the following conditions: leukemia, 64-72%; other types of disseminated neoplastic disease, 36-78%; regional ileitis, 45%; ulcerative colitis, 38%; tuberculosis, 33%; during the 1st wk after major surgery, 33%.BJC2 was found in the urine by immunoelectrophoresis in 10% of patients with neoplastic disease and was not observed in urine of other patients or in human plasma. Amino acid composition, carbohydrate content, and antigenic specificity indicate BJC1 is a previously unrecognized member of the system of normal human plasma glycoproteins. Like certain other glycoproteins, its plasma concentration frequently increases in patients with neoplastic disease, chronic inflammatory disease, or tuberculosis and after surgery. Because molecular weight is 29,000, increased plasma concentration readily causes its appearance in the urine.


Fine structure of the newt parathyroid gland after EDTA administration: mechanism of formation and extrusion of secretory granules.


Design and evaluation by nitrogen balance and blood aminograms of an amino acid mixture for total parenteral nutrition of adults with gastrointestinal disease. This study was undertaken to assess the efficacy of an amino acid mixture formulated for intravenous use from estimates of requirements for essential amino acids of human adults, and from data previously derived from a study using casein hydrolysate as the amino acid source. This mixture contained 39.4% essential amino acids, with glycine, alanine, arginine, histidine, and proline selected to supply the nonessential nitrogen. Nitrogen balance and blood aminograms were measured in six adult patients with gastrointestinal disease, fed intravenously for 1 wk at each of three levels of amino acid intake, while all other nutrients were constant and adequate in relation to body weight. Four of the patients were then fed 1.0 g/kg of egg or meat protein orally for 1 wk at the end of the 3 wk study, while all nutrients other than the amino acids were fed intravenously. Average nitrogen balances of -0.8, 0.5, and 2.2 g/day were observed when the amounts of amino acids infused were 0.25, 0.5, and 1.0 g/kg, respectively. Fasting levels of threonine, glycine, cystine, methionine, isoleucine, tryptophan, and arginine, but not of the other amino acids, increased with the increment in input, although the levels were generally lower than normal. The increase in blood concentration observed during infusion was similar for each of the essential amino acids, indicating that the supply of each of the amino acids was much better balanced for utilization than casein hydrolysate. However the blood aminograms did suggest that some changes in composition of the mixture for the malnourished adult were desirable, such that the amino acid mixture resembled that needed by the normal growing child. Nitrogen balance in the four patients fed orally with high-quality protein averaged 2.0 g/day, showing that an amino acid mixture given intravenously, provided it is well-balanced, can be utilized as efficiently as protein given orally. It is concluded that the prediction of oral requirements for amino acids should be combined with observations on changes in blood aminograms during infusion over a range of amino acid input in the development of amino acid mixtures for intravenous feeding.


Letter: Crystal-like structure in the sporulating cells of Bacillus subtilis 168.


Cancer mortality in Blacks.

Cancer mortality in Blacks.


Thyroxine-binding proteins in sheep serum.


Systematic head and neck examination.

Systematic head and neck examination.


Effect of thyroidectomy on the concentration of plasma thyroxine-binding proteins in sheep.


Evaluation of nitroglycerin ointment.

Evaluation of nitroglycerin ointment.


Emotional crises and cancer.

Emotional crises and cancer.


The simultaneous effect of protein-calorie malnutrition on weight and height velocity.


Minicells of Bacillus subtilis: a new bacteriophage-blocking agent. Bacteriophage SP01, SP17, and phi29 rapidly absorb irreversibly to minicells of Bacillus subtilis but do not produce a lytic cycle in minicells.


Activation of a latent measles virus infection in hamster cells. The characteristics of infectious measles virus released from latently infected hamster embryo fibroblast cells are described. Low levels of virus were released spontaneously when the cultures were incubated at 37 C; this phenomenon was observed 19 passages after the cells had been exposed to the virus and has continued through cell passage 45. The virus yield could be significantly increased by cocultivation of the hamster cells with BSC-1 cells or incubation of the latently infected cells at 33.5 C rather than at 37 C. Measles virus released after cocultivation demonstrated increased cytopathology in cell culture and reduced temperature sensitivity when compared to the virus released at 33.5 C. After cell passage 45, there was an increase in spontaneous release of virus. However, the viruses recovered by cocultivation or temperature release after cell passage 45 were nearly identical. These observations suggest a possible mechanism for measles virus activation in cells latently infected with this virus.


Classics in oncology. Matthew Baillie (1761-1823).


Increased nerve-growth-stimulating activity in disseminated neurofibromatosis.


Editorial: Nerve growth in neurofibromatosis.


Letter: Enhancement of enzymatic activity in Fabry's disease.


Letter: Priapism after phenoxybenzamine in a patient with Fabry's disease.


Letter: Cost of health services in Sweden and England.


Endotoxic shock.

Endotoxic shock.


Endocavity irradiation of early rectal cancers for cure: a series of 123 cases.


Preoperative megavoltage radiotherapy for advanced carcinoma of the rectum.


Postgraduate education and training in public health. Report a WHO Expert Committee.


Environmental and health monitoring in occupational health. Report of a WHO Expert Committee.


Bioavailability of drugs: principles and problems. Report of a WHO Scientific Group.


Malaria control in countries where time-limited eradication is impracticable at present. Report of a WHO Interregional Conference.


Gold levels in serum during the treatment of rheumatoid arthritis with gold sodium thiomalate.


Protein selectivity in the assessment of patients with proteinuria.


Aetiological factors in ovine posthitis.


Degradation of hyaluronic acid by baboon sex-skin tissue.


Effect of sulfonylureas on the secretion of pancreatic immunoreactive glucagon and enteric glucagon-like immunoreactivity.


A comparative evaluation of the ultrastructural changes following induced cerebral infarction in the squirrel monkey and baboon.


Biosynthesis of the crystal protein of Bacillus thuringiensis var. tolworth. 1. Kinetics of formation of the polypeptide components of the crystal protein in vivo.


Occurrence of 2-amino-2-deoxy-hexuronic acids as constituents of Vibrio parahaemolyticus K15 antigen.


Biosynthesis of the crystal protein of Bacillus thuringiensis var. tolworth. 2. On the relation of transcriptional and translational events in the growth cycle.


Purification and properties of an iodide peroxidase from submaxillary gland of goat.


Evaluation of alclofenac treatment regimes in man.


Virulence of multidrugresistant Mycobacterium tuberculosis.


General and specific effects of modifiers of mutant expression.


Growth hormone secretion in protein-calorie malnutrition.


Response of rhesus monkeys to LRH throughout the ovarian cycle.


Characterization of a human acute phase protein found in association with rubella virus infection. A precipitating antigen, rho, was first detected in the blood of persons with rubella and in rubella virus-infected cell culture fluids (1). Partially purified antigens from both sources were examined and shown to have similar properties, although antigen from serum sedimented more heterogeneously, with estimated coefficients from 15 to 21 S, while that from culture fluids sedimented in the 11-14 S region. In each case, antigen was located in the beta-1 zone after electrophoresis in agarose, and at a density of 1.305 g/ml after centrifugation in CsCl. Stability characteristics were typical of protein antigens. Immunofluorescent microscopy revealed that rubella virus induced the appearance of rho antigen scattered throughout the cytoplasm of infected cells. When cells containing antigen were exposed for 24 h to 5 microg/ml actinomycin D rho was no longer detectable, indicating the probable cellular origin of the antigen. Also, titers in medium of infected cultures showed a reduction after actinomycin treatment, but levels of the virus-specified antigen, iota, were relatively unaffected. Rho appears to be a protein common to man and many animals. In vitro, it was induced by rubella virus and by adenovirus. In vivo, rho titers were shown to be elevated after rubella virus infection and, to a lesser extent, after infection with certain other viruses. High titers were also demonstrated in women late in pregnancy and in patients with rheumatoid arthritis. In man and the chimpanzee, the appearance and decline of rho in the blood after rubella virus infection were temporally similar to the patterns of CRP, although rho seemed to be a more sensitive indicator of infection. The data presented indicate that rho is a newly recognized acute phase protein inducible by certain virus infections and by other unidentified stimuli present prominently in pregnancy and rheumatoid arthritis.


New, third class of amyloid fibril protein. An unusual protein AR was isolated from the amyloid fibril preparation derived from a patient with primary amyloidosis. Protein AR was unique in its antigenicity, and revealed no structural identity with any known amyloid proteins or with immunoglobulin chains or fragments. Thus a new third class of amyloid fibril proteins besides the immunoglobulin light-chain variable region fragments and the nonimmunoglobulin protein AS, has been characterized. A component antigenically related to protein AR was found in the serum of the patient.


Evaluation of thyrotropin-releasing hormone as a potential antidepressant agent in the conscious dog.


Dental defects in hamsters infected with minute virus of mice.


Localization of free and bound secretory component in human intestinal epithelial cells. A model for the assembly of secretory IgA. Antibody reagents were made specific for each of the two forms of human SC, FSC and BSC, which is an integral part of the sIgA molecule. With the fluorescent antibody method the cytological and histological localization of FSC, BSC, and alpha-chains has been studied in various human mucous membranes. SC was present in columnar epithelial cells of the intestines and in the cells of serous acini of bronchial and salivary glands. In contrast, SC was not found in intestinal goblet cells or cells of mucous acini of bronchial and salivary glands. In the columnar epithelial cells of the small and large bowel, FSC was present most prominently in the Golgi zone, and much less prominently in the apical cytoplasm. On the other hand, BSC and alpha-chains were located only in the apical cytoplasm in an overlapping manner. The results favor a model in which sIgA is assembled inside epithelial cells from SC, which was synthesized in the same cell, and IgA, which entered the epithelial cell after synthesis in and secretion by a plasma cell.


Dental pulp regeneration aided by blood and blood substitutes after experimentally induced periapical infection.


Analysis of histologic criteria commonly used in pulp studies.


Antigenicity and stilbamidine resistance in Trypanosoma brucei rhodesiense.


Letter: Serology and antimicrobial sensitivity of meningococci isolated in Uganda.


Letter: Acquired resistance to Schistosoma haematobium in the baboon (Papio anubis).


Enzyme variants in primate malaria parasites.


Letter: Identification of guinea-worm species.


Cytotoxic substances in sera from P. knowlesi-infected rhesus monkeys.


6-Phosphogluconate dehydrogenase activity in malaria-infected erythrocytes.


Eperythrozoon and Haemobartonella in primates.


Acquired resistance to Schistosoma haematobium in the baboon.


Malaria parasites in Presbytis aygula. New host record of Hepatocystis semnopitheci.


Malaria parasites in Callosciurus nigrovittatus.


Polychromophilus species in insectivorous bats.


Hymenopteran egg-parasite of triatomine bugs.


Malaria parasites of domestic fowl in Sabah.


A multi-species malaria antigen for use in the indirect fluorescent antibody test.


Two types of schizonts of Hepatocystis sp., a parasite of insectivorous bats in the Congo-Brazzaville.


Vesicoureteral reflux in adults. A twelve-year study of 122 patients.


Chemotherapy for bladder cancer.

Chemotherapy for bladder cancer.


The effects of anesthetics on permeability to water of the inactivated toad bladder.


Benzodiazepines in the treatment of epilepsy. A review.


Health trends in three Asian countries--Ceylon, Malaysia and Singapore.


Fine structure and function of ocular tissues. The ciliary body.


Fine structure and function of ocular tissues. The vitreous.


Fine structure and function of ocular tissues. Extraocular muscle.


Specialized techniques in electron microscopy.


An ergot alkaloid preparation (Hydergine) in the treatment of presenile brain atrophy (Alzheimer's disease): case report.


Patient non-compliance within the context of seeking medical care for arthritis.


Long-term consequences of vestibular ablation by laser in a monkey.


About quantitative studies on the cerebral cortex.


Hydergine for cerebral arteriosclerosis.


Proceedings: Penicillins: use of an animal model to predict bioavailability.


Estimation of molecular weights of rat liver ribosomal proteins.


The proteins of rapidly labelled polysomal mRNP of rat liver.


Study of the in-vitro sensitivity of meningococci and its implications in prophylaxis.


Studies on serum antigens in carp.

Studies on serum antigens in carp.


Effects of genetic background on the competition between the asc and FM6 chromosomes of Drosophila melanogaster.


Flavor aversions produced by contingent drug injection: relative effectiveness of apomorphine, emetine, and lithium.


Preservation of 23-S chloroplast RNA as a single chain of nucleotides.


Neuronal alteration and reinnervation following axonal regeneration and sprouting in mammalian spinal cord.


Morphological plasticity of central adrenergic neurons.


Anatomical and physiological correlates of plasticity in the central nervous system.


The alteration of aversive thresholds with cholinergic and adrenergic agents.


Vitamin E in angina pectoris.

Vitamin E in angina pectoris. The claim that the symptoms of angina pectoris can usually be relieved by large doses of vitamin E has been reinvestigated by means of a randomized double-blind trial. The trial lasted nine weeks and consisted of two parts. One part was conducted as a regular double-blind trial involving 40 patients, half of whom received 3200 IU of vitamin E daily, while an equal number received an indistinguishable placebo. The second part of the trial involved 15 patients who were already taking a regular daily dose of between 400 and 2400 IU of vitamin E. Eight patients were assigned the same (or a larger) dose of vitamin E, while seven received placebo. Neither part of the trial yielded statistically convincing evidence that vitamin E is of value in the treatment of angina, but a small beneficial effect could not be ruled out. Taken in conjunction with the positive (but statistically non-significant) results obtained in the only other double-blind trial of vitamin E ever carried out on angina, and the encouraging results reported by other investigators in the treatment of intermittent claudication, it is suggested that further double-blind trials are justified.


Estimation of serum and red cell folate by a simple radiometric technique.


Computer reduction and analysis of neurophysiological data with special emphasis on evoked potential frequency analysis.


Effects of scopolamine, amphetamine and benzodiazepines on conditioned suppression.


Stereoscopic form disappearance in temporal lobe dysfunction.


Ionophore A23187, calcium and contractility in frog eggs.


Photoreactivation of excystment delay in Colpoda, induced by far ultraviolet radiation.


What is a case of tuberculosis? The tuberculosis case spectrum in eight countries evaluated from 1235 case histories and roentgenograms.


Isoniazid chemoprophylaxis of latent primary tuberculosis: in five trial centres in France from 1959 to 1969.


The effect of different preparative methods on nuclear shape in the surface epithelium of the hamster ovary.


Cellular changes in the lateral geniculate nucleus of infant monkeys after suture of the eyelids.


Evaluation of an antiasthmatic agent cromolyn sodium (Aarane, Intal).


Evaluation of post-episiorrhaphy pain: polyglycolic acid vs catgut sutures.


Brenner tumor.

Brenner tumor.


Intubation: the simple approach to bleeding from the mesenteric small bowel.


Epidemiology of heroin dependency among soldiers in Vietnam.


Opiate addiction and military psychiatry to the end of World War II.


Nursing practice in a medical research setting.


Dental emergencies occurring among Navy-Marine personnel serving in Vietnam.


Frequency of hepatitis-associated antigen among drug users in the Naval service.


Optic chiasmatic arachnoiditis: improvement of rapidly failing vision following surgical lysis of chiasmatic adhesions and postoperative steroid therapy.


Injuries to divers by marine animals: a simplified approach to recognition and management.


Editorial: Communicating clinical research in nursing.


Immunoprophylaxis of meningococcal infection.


Psychiatric perspectives of the Vietnam veteran.


Oral lymphogranuloma venereum and cervical lymphadenopathy. Case report.


Transformation of an R-factor from Pseudomonas aeruginosa into Rhizobium trifolii.


A comparison of direct and diluted sputum culture results.


Experimental allergic encephalomyelitis in hamsters.


Species specificity in the TSH response to the administration of L-dopa.


The neural basis of visually guided behavior.


Pregnancy-associated serum antigens in the rat and mouse.


Platelet aggregation by large molecules.


Relationship of the localization of autosomal lethal mutations to their concentrations in natural populations of Drosophila melanogaster.


Studies on reaction between thrombin and actin.


Repair of UV-damaged DNA by competent and incompetent Bacillus subtilis cells.


Urethral diverticulum in adult female. Clinical aspects, operative procedure, and pathology.


Bladder diverticulectomy. With aid of inflated Foley balloon catheter.


Psychophysical studies of monkey vision. I. Macaque luminosity and color vision tests.


Psychophysical studies of monkey vision. II. Squirrel monkey wavelength and saturation discrimination.


Psychophysical studies of monkey vision. 3. Spatial luminance contrast sensitivity tests of macaque and human observers.


Fate of exogenous DNA in Neisseria meningitidis. I. Preparation of 15N-donor DNA and its fate in transformation.


Fate of exogenous DNA in Neisseria meningitidis. 2. Influence of acriflavin and ethidium bromide and significance of genetic competence on the fate of 15N DNA.


Identification and quantitation of precipitins against Pseudomonas aeruginosa in patients with cystic fibrosis by means of crossed immunoelectrophoresis with intermediate gel.


Wall teichoic acids in animal Staphylococcus aureus strains determined by precipitation.


An easy way of improving the flatness of the radiation from a linear accelerator.


Origin of radiation released serotonin in rabbits and mice.


Plasma and leukocyte ascorbic acid levels in the elderly.


Comparison of fibrin hydrolysates and crystalline amino acid solutions in parenteral nutrition.


The matabolic fuel regulatory system: implications for protein-sparing therapies during caloric deprivation and disease.


Nutritional hazards of food taboos and preferences in Mid-West Nigeria.


Opponent processes as a model of neural organization.


Experimental carbon monoxide encephalopathy in the primate I. Physiologic and metabolic aspects.


Experimental carbon monoxide encephalopathy in the primate. II. Clinical aspects, neuropathology, and physiologic correlation.


SSPE virus infections in suckling hamsters. Modification by intracerebral administration of poly I-C.


Pulmonary alveolar proteinosis and Nocardia brain abscess. Report of a case.


Studies of a transplantable canine sarcoma.


Oncorna type virus particles in a tumor of a rhesus monkey.


Effects of myelopoietic factors isolated from rat serum on murine myelogenous leukemia.


Gross virus leukemia in the rat: kinetics of infection and immunity.


Measurement of resistance to air flow in the nose in a trial with sodium cromoglycate (BP)solution in allergen-induced nasal stenosis.


The localization of "ordinary" sex-linked genes in section 20 of the polytene X chromosome of Drosophila melanogaster.


Serum IgE levels and the use of sodium cromoglycate (BP) in hay fever: a study of forty-six patients from a single general practice.


Trace-element analysis in hair: an evaluation.


Rapid ultramicro direct determination of erythrocyte lead concentration by atomic absorption spectrophotometry, with use of a graphite-tube furnace.


Notes on the ecology and behaviour of the Angola colobus (Colobus angolensis P. L. Sclater 1860) in N.E. Tanzania.


Wrist mechanism and locomotor behavior of Dryopithecus (Proconsul) africanus.


Interactions between adult female and infant howling monkeys (Alouatta palliata).


Condensation of chromosomes during mitotic prophase. Whole mount electron microscopy of cerebral ganglion cells of Drosophila.


Quality and intensity of sugar solutions as determinants of ingestion by the rhesus monkey (Macaca mulatta).


Tricomplex, a new type of autosome complement in Drosophila melanogaster.


Heritability of recombination frequency. Observations on statistical complications and report on small-scale tests in Drosophila.


Genetic load in a repeatedly irradiated Drosophila melanogaster population.


Localization of genes controlling radioresistance in Drosophila melanogaster.


Characterization of a meiotic control gene affecting recombination in Drosophila melanogaster.


The organization of chromosome fibrils in salivary-gland chromosomes of Drosophila melanogaster. Light microscopical observations of the effect of acetic acid.


Oxidation of catechol in plants. II. Enzymic conversion of catechol to diphenylenedioxide 2,3-quinone in the leaves of Tecoma stans L.


Purification and properties of hetero-beta-galactosidases from monkey liver and intestine.


Neurofibromatosis--von Recklinghausen's disease in podiatric medicine. A review and report of two cases.


Documentation of auxotrophic mutation in blue-green bacteria: characterization of a tryptophan auxotroph in Agmenellum quadruplicatum. A tryptophan-requiring auxotroph of Agmenellum quadruplicatum strain BG1, a species of blue-green bacteria, was isolated by means of a nitrosoguanidine-penicillin procedure. Its growth characteristics were determined, and the enzymological block was identified in the A activity of tryptophan synthetase. Starvation of the auxotroph for tryptophan resulted in the derepression of the synthesis of all five enzymes. The first four enzymes derepressed 2- to 3-fold, and tryptophan synthetase B derepressed 20-fold. In the parental prototroph, BG1, anthranilate synthetase was active in crude extracts with ammonia as the amino donor reactant, but not with glutamine.


Nature of the elimination of the penicillinase plasmid from Staphylococcus aureus by surface-active agents. Growth of Stapylococcus aureus in various ionic surface-active agents resulted in loss of the ability to produce penicillinase, whereas growth in nonionic surface-active agents had no effect on penicillinase production. The curing effect of various alkyl sulfates was found to be dependent upon the chain length. Curing by surface-active agents could be inhibited by magnesium. Reciprocal transduction experiments showed that curing by a surface-active agent was a property of the plasmid, not of the bacterial strain in which the plasmic resides.


Deoxyribonucleic acid distribution in Bacillus subtilis independent of cell elongation. A temperature-sensitive DNA(-) mutant of Bacillus subtilis has been studied during the resumption of deoxyribonucleic acid (DNA) synthesis following a 45 to 30 C temperature shift. For several hours after return to 30 C, DNA synthesis proceeds although the cells fail to elongate appreciably. Autoradiographs of cell populations synthesizing DNA during the recovery period demonstrate that DNA can become distributed to previously unoccupied regions along the cell length. By varying the labeling regime, newly synthesized DNA as well as DNA present at the time of transfer from 45 to 30 C were followed independently. Measurements of the percent of cell length covered by grains ((3)H-thymine in DNA) demonstrate the progressive refilling of DNA-vacant cell regions by both newly synthesized and original DNA. These data indicate that cell surface growth is not an absolute requirement for segregation of bacterial DNA.


Initiation of Bacillus subtilis ribonucleic acid polymerase on deoxyribonucleic acid from bacteriophages 2C, phi 29, T4, and lambda. Ribonucleic acid (RNA) synthesis primed by bacteriophage T4 or lambda deoxyribonucleic acid (DNA) with Bacillus subtilis RNA polymerase is severely inhibited by high ionic strength. In contrast, RNA synthesis on B. subtilis bacteriophage 2C, SPO1, or phi29 DNA is only moderately affected under similar conditions. The basis of this inhibition lies in the inability of the enzyme to initiate RNA chains with adenosine triphosphate or guanosine triphosphate (ATP, GTP). Binding to templates and the rate of catalysis in high salt after initiation do not seem to be affected. Incorporation of gamma-(32)P-ATP and GTP under a variety of conditions suggests that the specificity of B. subtilis RNA polymerase is different from that of the Escherichia coli enzyme and that it recognizes few promoters on T4 and lambda DNA. Although B. subtilis RNA polymerase initiates RNA chains primarily with ATP or GTP, initiations with pyrimidines can occur on DNA molecules in which hydroxymethyluracil replaces thymine. RNA synthesis on denatured DNA does not seem to be inhibited by high ionic strength, and on native T4 or lambda DNA the inhibition of initiation at constant ionic strength is inversely but not linearly proportional to the ionic radii of cations used to stabilize bihelical DNA to denaturation.


Comparative immunological and enzymatic study of the tryptophan synthetase beta 2 subunit in the Enterobacteriaceae. The beta(2) subunits of tryptophan synthetase, formula alpha(2)beta(2), from Escherichia coli, Shigella dysenteriae, Enterobacter aerogenes, Salmonella typhimurium, and Serratia marcescens were compared by three criteria. (i) alphabeta association constants for the various beta(2) subunits and E. coli alpha subunit varied between 3.6 x 10(8)m(-1) for E. coli and 0.33 x 10(8)m(-1) for S. marcescens; values for the other organisms were intermediate. (ii) Antiserum neutralization of the beta(2) subunit enzyme activity using anti-E. coli beta(2) serum showed significant cross-reaction among the organisms (E. coli, 1.0; S. dysenteriae, 0.98; S. typhimurium, 0.67; E. aerogenes, 0.61; S. marcescens, 0.42). (iii) Quantitative microcomplement fixation showed E. coli beta(2) and S. marcescens beta(2) subunits to have an index of dissimilarity of 1.8 while the other organisms had intermediate indexes. Similar complement fixation data were obtained with antisera from separate rabbits and from first course and boost sera. These findings suggest that the general surface structure and the respective alpha subunit binding site of the beta(2) subunits from these Enterobacteriaceae have been strongly conserved.


Genetically controlled removal of "spore photoproduct" from deoxyribonucleic acid of ultraviolet-irradiated Bacillus subtilis spores. Previous genetic analysis indicated that at least two genes determine the ultraviolet (UV) sensitivity of Bacillus subtilis spores. The present study shows that these genes independently control two distinguishable processes for removing UV-induced spore photoproduct (5-thyminyl-5,6-dihydrothymine, or TDHT) from spore deoxyribonucleic acid. The first, is a spore repair mechanism by which TDHT is removed rapidly without appearing in acid-soluble form. This mechanism, which is demonstrated in both UV-resistant and excision-deficient strains, operates to a certain extent during germination without requiring vegetative growth. The second, demonstrated in a mutant which lacks the first mechanism, removes TDHT relatively slowly and only if germinated spores are allowed to develop toward vegetative cells. The latter mechanism appears identical to excision-resynthesis repair, since the mutation abolishing it renders the irradiated vegetative cells incapable of removing cyclobutane-type pyrimidine dimers. Blocking either one of these mechanisms only slightly affects the UV sensitivity of spores, but blocking both prevents TDHT removal and gives high UV sensitivity.


Phenoloxidase activity in mycelia carrying modifier mutations that affect sporocarp development in Schizophyllum commune. Phenoloxidase activity was studied in mycelia carrying mutations that affect sporocarp formation. The results suggest that phenoloxidase activity may be essential to, but is not sufficient for, the fruiting response in Schizophyllum.


Division site regulation in a temperature-sensitive mutant of Bacillus subtilis. Division site location was measured in Bacillus subtilis clones grown from spores at 30 and 45 C. Regulation of division location is lost in mutant 168ts-151 at 45 C.


Time course of purine nucleoside phosphorylase occurrence in sporulation of Bacillus cereus. Purine nucleoside phosphorylase (EC 2.4.2.1) from Bacillus cereus T was examined at hourly intervals during growth and sporulation. The enzyme has maximal activity in extracts prepared from cells during stages I and II. The activity during exponential growth is only 6.6% of the maximum and that in free spores is only 3.3%. Conservation of the purine nucleoside phosphorylase during sporulation is apparent as shown by the gradual increase in heat resistance.


Mapping of rod mutants of Bacillus subtilis. Nine class A salt-dependent rod mutants were mapped on the Bacillus subtilis genome by PBS1-mediated transduction. They are distributed into two small linkage groups designated rod B and rod C; mutations in rod B are over 80% cotransducible with pheA and different mutations in rod C are 12 to 21% cotransducible with hisA. It is established that neither rod B nor rod C is linked by transformation to the other identified rod mutations present in 168-ts-200B and 8332 glu(-). It is hypothesized that salt-dependent mutations are due to enzyme alterations which are corrected by high salt concentrations.


Synthesis of pulcherriminic acid by Bacillus subtilis. The pathway of pulcherriminic acid synthesis in Bacillus subtilis strains AM and AM-L11 (a leucine-requiring auxotroph) was investigated. Determinations of radioactivity in pulcherriminic acid synthesized by cells growing in media containing (14)C-labeled amino acids indicated that B. subtilis produced pulcherriminic acid from l-leucine. The organism utilized the carbon skeletons of two l-leucine molecules to synthesize one molecule of pulcherriminic acid. Similar results were obtained with starved cell suspensions. Growing cells formed significant amounts of pulcherriminic acid only in media including a carbohydrate such as starch. However, carbohydrate carbon was not required for the synthesis of pulcherriminic acid molecules. Data obtained with cell suspensions supported the hypothesis that cyclo-l-leucyl-l-leucyl is an intermediate in pulcherriminic acid biosynthesis and indicated that molecular oxygen is required for the conversion of cyclo-l-leucyl-l-leucyl to pulcherriminic acid. A pathway for the synthesis of pulcherrimin from l-leucine in B. subtilis is proposed.


Perinatal tips.

Perinatal tips.


Isotopic study of control of the lysine biosynthetic pathway during sporulation of Bacillus cereus. The extent of incorporation of aspartate into dipicolinic acid and into various amino compounds was determined in Bacillus cereus at various times before, during, and near the end of synthesis of dipicolinic acid. The purpose of this study was to gain further information on the in vivo control of the biosynthesis of amino acids derived from aspartate. Control of the lysine biosynthetic pathway was of particular interest with regard to sporulation, owing to the important role of diaminopimelate and dipicolinate in the structure of the spore. As synthesis of dipicolinate was initiated, incorporation of carbon derived from aspartate was funneled preferentially into this compound as compared with others of the aspartate group. Incorporation into lysine essentially stopped just before the synthesis of dipicolinate began. This is consistent with the previously observed disappearance at this time of diaminopimelic acid decarboxylase in cell-free extracts. Synthesis of diaminopimelate continued during the time of synthesis of dipicolinate. The previous suggestion that diaminopimelate might exert negative control on one of the enzymes between dihydrodipicolinate and diaminopimelate is thus considered unlikely. The possibility is discussed that synthesis of dipicolinate is favored by an increase in the rate of synthesis of dihydrodipicolinate rather than by a block in its rate of utilization.


Induction of psychological death in rhesus monkeys.


Bridging a cultural gap for better patient care.


Shoulder girdle neuritis: a clinical and electrophysiological evaluation.


Tropical sprue in South Vietnam.

Tropical sprue in South Vietnam.


An experiment in postgraduate medical education: an orientation program for residents in clinical research.


Acute exertional rhabdomyolysis: a syndrome of increasing importance to the military physician.


Case for diagnosis.

Case for diagnosis.


A fatal case of hexachlorophene poisoning.


Multiple intracranial arterial aneurysms: current thoughts about diagnosis and treatment.


Drug induced intrahepatic cholestatic jaundice: case reports.


Editorial: The uniformed services medical school.


Vesicoureteral reflux: a review.

Vesicoureteral reflux: a review.


Sitting on a basketball: how it feels to be a paraplegic.


The dissociation between renal HCO3- reabsorption and H+ secretion in the skate, Raja erinacea.


Letter: The antimalarial activity of RC-12 against gametocytes and sporogony of Plasmodium cynomolgi.


Letter: Circadian rhythm in the parasitaemia of the primate haemoflagellate, Trypanosoma minasense.


Polycholorinated biphenyls. VI. 2,3,7,8-tetrachlorodibenzofuran, a critical byproduct in the synthesis of 2,2',4,4',5,5'-hexachlorobiphenyl by the Ullmann reaction.


Esophageal intramural diverticulosis: a clinical and pathological survey.


Inhibition of metabolism by hydrostatic pressure: what limits microbial growth?


Glycyl-L-leucine hydrolase, a versatile 'master' dipeptidase from monkey small intestine. 1. A highly active and electrophoretically homogeneous dipeptidase was purified from the soluble extracts of monkey small-intestinal mucosa. 2. By gel-filtration studies the molecular weight of the enzyme was found to be 107000. It is composed of two identical, subunits of molecular weight 54000. 3. A paper-chromatographic method of dipeptidase assay was developed to overcome some of the difficulties encountered in the generally used spectrophotometric procedure. By using this method, the K(m) and k(0) values of a few substrates were determined. 4. The substrate specificity of the enzyme was investigated in great detail with substrates of a wide range of possible structural types. The enzyme hydrolyses a very large proportion of the range of dipeptides tested. This enzyme, which exhibits such a wide range of action, has been termed the ;master' dipeptidase of the intestine. Glycylglycine, glycyl-l-proline, glycyl-l-histidine, l-prolylglycine and some of the arginine- and aspartic acid-containing dipeptides were not substrates and are possibly hydrolysed by other peptidases. These results therefore suggest that in the intestine the number of dipeptidases is rather limited. 5. In the light of these findings, the implications on the role of dipeptidases in intestinal peptide transport are discussed.


Purification and some properties of rabbit anti-ovalbumin. Unlike previous reports that the ovalbumin-anti-ovalbumin complex did not dissociate completely in acid media, our results showed complete dissociation of the complex both in 1.2m-acetic acid, pH2.3, and in KCl-HCl, pH2.2, I 0.06. Thus Sephadex chromatography of the solution obtained by dissolving the antigen-antibody precipitate in these media repeatedly gave two peaks corresponding to anti-ovalbumin and ovalbumin. Further, gel-diffusion and immunoelectrophoresis experiments showed that the phosphate groups of ovalbumin are not involved in the antigenic sites. The antibody thus purified was more easily precipitated than previous preparations. The molecular weight and Stokes radius of the antibody were calculated from its gel-filtration behaviour and were found to be 148000 and 4.8nm respectively. The molecular weight determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis was essentially similar (about 0.7% lower).


Purification and some properties of cytoplasmic aspartate aminotransferase from sheep liver. 1. A procedure for the purification of the cytoplasmic isoenzyme of aspartate aminotransferase from sheep liver is described. 2. The purified isoenzyme shows a single component in the ultracentrifuge at pH7.6 and forms a single protein band on agar-gel electrophoresis at pH6.3 or 8.6, as well as when stained for protein or activity after polyacrylamide-gel or cellulose acetate electrophoresis at pH8.8. 3. Immunoelectrophoresis on agar gel yields only one precipitin arc associated with the protein band, with rabbit antiserum to the purified isoenzyme. By immunodiffusion, cross-reaction was detected between the cytoplasmic isoenzymes from sheep liver and pig heart, but not between the cytoplasmic and mitochondrial sheep liver isoenzymes. 4. The s(20,w) of the enzyme is 5.69S and the molecular weight determined by sedimentation equilibrium is 88900; 19313 molecules of oxaloacetate were formed/min per molecule of enzyme at pH7.4 and 25 degrees C. 5. The amino acid composition of the isoenzyme is presented. It has about 790 residues per molecule. 6. The holoenzyme has a maximum of absorption at 362nm at pH7.6 and 25 degrees C. 7. A value of 2.1 was found for the coenzyme/enzyme molar ratio. 8. The purified enzyme revealed two bands of activity on polyacrylamide-gel electrophoresis at pH7.4 and an extra, faster, band in some circumstances. These bands occurred even when dithiothreitol was present throughout the isolation procedure. 9. Three main bands were obtained by electrofocusing on polyacrylamide plates with pI values 5.75, 5.56 and 5.35. 10. Structural similarities with cytoplasmic isoenzymes from other organs are discussed.


Polymerization of proteins with glutaraldehyde. Soluble molecular-weight markers. Glutaraldehyde is well known for its ability to react with proteins and to produce insoluble cross-linked aggregates. In contrast with this situation, conditions are described here which yield covalently linked soluble protein oligomers. The procedure is applicable to a wide range of proteins, and by slight variation in the reaction conditions, soluble polymers in the molecular weight range 3x10(4)-2x10(7) were produced. The products are valuable as molecular-weight markers, e.g. in sodium dodecyl sulphate-polyacrylamide-gel electrophoresis. The inherent similarities of these oligomers make them superior to commercial molecular-weight protein markers, which may have marked differences in composition and charge.


Use of affinity chromatography for the quantitative study of acceptor-ligand interactions: The lactose synthetase system. From the effects of N-acetyl-d-glucosamine and d-glucose on the elution of the A protein of human lactose synthetase from a column of Sepharose-alpha-lactalbumin, values of 200m(-1) and 0.57m(-1) are deduced for the association constants describing the interaction between the enzyme and the respective monosaccharides.


The size of the Langerhans' islets in infants with severe jaundice of varying etiology.


Effects of bilirubin loading of pregnant rats on hepatic UDP-glucuronyltransferase activity in the offspring.


The structure of the mitochondrial matrix.


Changes of the ultrastructural pattern of the nucleus and nucleolus in regenerating rat liver.


Organization of amphibian extraocular muscles.


Ultrastructure of neurosecretory cells in the brain of the crab Rhithropanopeus harrisi (Gould).


Periodicity of infectivity of plasmodial gametocytes: the "Hawking phenomenon".


Experimental infection of African baboon (Papio cynocephalus) with Brugia malayi.


A new biological assay, utilizing parasitic protozoa, for screening carcinogenic substances which induce bladder cancer in man and other mammals.


"Contractile interstitial cells" in pulmonary alveolar septa: a possible regulator of ventilation-perfusion ratio? Ultrastructural, immunofluorescence, and in vitro studies. In the lungs of healthy rats, humans, lambs, and monkeys, about 50% of the alveolar interstitial cells-resembling fibroblasts-contain bundles of fibrils measuring 30-80 A in diameter. Immunofluorescence studies on frozen sections of rat lung demonstrate that many interstitial cells bind sera containing antiactin antibodies. On account of these two sets of findings and our additional in vitro studies suggesting alveolar tissue contraction due to hypoxia or epinephrine, we postulate that the alveolar septa contain contractile cells different from that of smooth muscle. For these cells we propose the name of "contractile interstitial cells." Such cells lie within the thick portion of the air-blood barrier and around the pre- or postcapillary vessels. Hence it is possible that they play a role in the autoregulation of ventilation/perfusion (V/Q) ratio, particularly in hypoxic pulmonary hypertension. These findings, demonstrating a contractile system other than bronchial and arterial smooth muscle, suggest that the alveolus should no more be conceived as a passive "organ."


Isolation of ciliated or unciliated basal bodies from the rabbit oviduct. Techniques have been developed for the isolation of basal bodies with cilia attached or for the isolation of only basal bodies from the rabbit oviduct. Oviducts are removed, cut open, and placed in an extraction medium composed of 0.25 M sucrose, 0.001 M EDTA, 0.025 M KCl, 0.02 M Hepes buffer pH 7.5, and 0.05% Triton X-100. After the oviduct is agitated in this medium on a Vortex mixer for (1/2) h, the lumenal cortex of each ciliated cell, containing 200-300 basal bodies with cilia attached, is released as a unit. The cortices and the intact nuclei, which are also released from the disrupted cells, form a pellet when the extraction medium is centrifuged at 600 g for 10 min. When cortices which contain only basal bodies are to be isolated, the oviduct is subjected to conditions which remove the cilia prior to being processed as above. The cilia are removed when the oviduct is placed in a medium of 0.25 M sucrose, 0.01 M CaCl(2), 0.02 M Pipes buffer pH 5.5, and 0.05% Triton X-100 and continuously agitated for 15 min on a Vortex mixer. The low pH and Ca(++) solubilize the transition region of the cilium and also prevent the cell from being disrupted. The cortices can be partially purified if the 600-g pellet is resuspended in 2.2 M sucrose pH 6.5 and centrifuged at 40,000 g for 2 h. Under these conditions, 85% of the nuclei form a pellet and the cortices float to the surface of the sucrose. In addition to the basal bodies or basal bodies with cilia, the cortices contain some adherent cytoplasm, a few fibers, and a few vesicles which may be remnants of mitochondria or endoplasmic reticulum. The structure of the cilia and the basal bodies isolated with either procedure is normal.


Calcium and flagellar response during the chemotaxis of bracken spermatozoids.


Porous substructure of the glomerular slit diaphragm in the rat and mouse. The highly ordered, isoporous substructure of the glomerular slit diaphragm was revealed in rat and mouse kidneys fixed by perfusion with tannic acid and glutaraldehyde. The slit diaphragm was similar in both animal species and appeared as a continuous junctional band, 300-450 A wide, consistently present within all slits formed by the epithelial foot processes. The diaphragm exhibited a zipper-like substructure with alternating, periodic cross bridges extending from the podocyte plasma membranes to a central filament which ran parallel to and equidistant from the cell membranes. The dimensions and spacing of the cross bridges defined a uniform population of rectangular pores approximately 40 by 140 A in cross section and 70 A in length. The total area of the pores was calculated to be about 2-3% of the total surface area of the glomerular capillaries. Physiological data indicate that the glomerular filter functions as if it were an isoporous membrane which excludes proteins larger than serum albumin. The similarity between the dimensions of the pores in the slit diaphragm and estimates for the size and shape of serum albumin supports the conclusion from tracer experiments that the slit diaphragm may serve as the principal filtration barrier to plasma proteins in the kidney.


Macromolecular requirements for the initiation and maintenance of DNA synthesis during the cell cycle of Tetrahymena pyriformis.


The subcellular distribution of lysosomal hydrolases in the Harding-Passey melanoma.


Long-term studies of iron metabolism in normal males: comparison of red blood cell radioactivity with whole-body counter data.


99mTc-tetracyclines: preparation and biological evaluation.


Use of 2-(4'-hydroxybenzeneazo)benzoic acid to study the binding of L-thyroxine to serum albumins.


Hepatitis B antigen-positive hepatitis in an Israeli family.


Biological significance of serum albumin binding parameters determined in vitro for clofibrate-related hypolipemic drugs: use of 2-(4'-hydroxybenzeneazo)benzoic acid to mirror L-thyroxine binding to and displacement from serum albumins.


Absence of an electron microscopic substructure in intact flagella of Proteus mirabilis and Bacillus subtilis.


In vitro hydrolysis of oxazepam succinate half-ester by a stereospecific soluble esterase from different animal species.


Proceedings: Physical data for electron therapy.


Skeletal fluorosis and dietary calcium, vitamin C and protein.


Proceedings: Elective radiotherapy of the cervical region in carcinoma of the head and neck.


The precipitin response induced in calves by a single experimental infection with Fasciola hepatica.


A new intradermal test antigen for the diagnosis of contagious bovine pleuropneumonia.


Calf immunoglobulins and congenital malformation.


IgA immunoglobulin levels in porcine sera and mammary secretions.


Plasma protein binding of digitoxin and digoxin in several mammalian species.


Abnormal behavioural patterns in dogs with cleft palates.


A sensitive, direct method for the measurement of water loss from body surfaces.


Fibrin deposits in the Kiil dialyser.

Fibrin deposits in the Kiil dialyser.


Food iron absorption in man. 3. Effect of iron salt, ascorbic acid and desferrioxamine on the isotopic exchange between native food iron and an extrinsic inorganic iron tracer.


Pathophysiologic basis of therapy for shock and trauma syndromes: use of sequential cardiorespiratory measurements to describe natural histories and evaluate possible mechanisms.


Radionuclide clearance techniques.

Radionuclide clearance techniques.


Therapy of nutritional failure.

Therapy of nutritional failure.


Radiopharmaceuticals for renal studies.

Radiopharmaceuticals for renal studies.


Uptake and removal of cortisol by canine erythrocytes in various electrolyte solutions.


Sarcosporidiosis in psittaciformes.

Sarcosporidiosis in psittaciformes.


Studies on hydatidosis in Indian buffaloes (Bubalus bubalis). I. Some serological observations in natural infections of hydatids in buffaloes.


Studies on hydatidosis in Indian buffaloes (Bubalus bubalis). II. Antigenicity of buffalo hydatid cyst fluid.


The fine structure of the conducting system of the monkey heart (Macaca mulatta). I. The sino-atrial node and the internodal connections.


Alteration of RNA synthesis in vitro with an endogenous regulating factor of cytodifferentiation of Streptomyces griseus.


Induced production of beta-galactosidase in Streptomyces griseus.


Effects of the oxathins DCMO and DCMOD on growth, proteins, oxidizing enzymes, and HCN production of low-temperature basidiomycete.


Arhinencephaly unilateralis, uveal coloboma, and lens reduplication.


Sequential studies of experimentally induced ovine listerial abortion: clinical changes and bacteriologic examinations.


Sequential studies of experimentally induced ovine listerial abortion: pathologic changes.


Ultrastructure of pulmonary alveoli of the calf.


Immunization of dogs against human chorionic gonadotropin.


Purification and characterization of hemolysin produced by Listeria monocytogenes.


Clearing technique for preparation and photography of anatomic specimens of blood vessels of female genitalia.


The formation of H2-pteroate analogs from PABA-antagonists in a cell-free system of E. coli.


Effect of metabolic inhibitors on mesenteric arterial smooth muscle.


Rubella: laboratory aspects.

Rubella: laboratory aspects.


Present status of vaccines for rubella.

Present status of vaccines for rubella.


Iatrogenic perforations of the esophagus. Therapeutic considerations.


Metabolic effects of hypoglycemic sulfonylureas. IV. Interference of sulfonylureas with mitochondrial oxidative phosphorylation.


Propargyl amine-induced irreversibel inhibition of non-flavin-linked amine.


Structures of reversible and irreversible complexes of thymidylate synthetase and fluorinated pyrimidine nucleotides.


Pseudothrombocytopenia: manifestation of a new type of platelet agglutinin.


Radioimmunoassay of calcitonin in human plasma.


Quantitation of serum IgG by solid-phase radioimmunoassay.


Variables in the laboratory diagnosis of Fabry's disease by measurement of methylumbelliferyl-alpha-galactosidase activity.


Measurement of alpha 1-antitrypsin in serum, by immunodiffusion and by enzymatic assay.


Quantitative determination of hepatitis B antigen by means of radial immunodiffusion.


Antigen binding and complement fixing activity of IgM molecules reassociated in the presence and absence of J chains.


Separation and characterization of cardiac antigen proteins.


Non-specific cytotoxicity by T cells activated with plant mitogens in vitro and the requirement for plant agents during the killing reaction.


Periovulatory time courses of circulating gonadotropic and ovarian hormones in the rhesus monkey.


The application of the maize-derived gene competition model to the problem of dosage compensation in Drosophila. The gene competition model, originally formulated from studies on the regulation of alcohol dehydrogenase activity in maize, is also applicable to the phenomenon of dosage compensation in Drosophila. The model accounts for the absence of dosage compensation in sex determination.


Mutation induction in the male recombination strains of Drosophila melanogaster. One group of the second chromosome lines isolated from a southern Texas population of Drosophila melanogaster, which has been known to show relatively high frequencies of male recombinations, was found to increase the frequency of sex-linked recessive lethal mutations from a control frequency of 0.18% to 1.63%. The second group, which showed a very much reduced frequency of male recombinations, was found to cause a slight increase to 0.48%, although it was not statistically significant. The first group was also tested for the recessive lethal mutation frequency in the second chromosome; the frequency increased from a control frequency of 0.28% to 2.82%. Mapping of a portion of the sex-linked lethals indicated a distribution along the entire X chromosome, although there was a tendency of clustering towards the tip of the X chromosome. One sex-linked lethal line so far tested was found to be associated with an inversion (approximate breakpoints, 14A-18A). It was suggested that the element causing male recombination might be similar to the hi mutator gene studied earlier by Ives (1950).


Developmental and genetic studies on kynurenine hydroxylase from Drosophila melanogaster. The level of kynurenine hydroxylase was measured throughout the development of wild type and the eye color mutants v, cn, st, ltd, cd, kar, w, ca, bri and p(P) of Drosophila melanogaster. In all cases except cn a bimodal distribution of enzyme activity during development was observed. Activity is initially detectable in second instar. A maximum is reached in early third instar. Activity declines prior to puparium formation. Shortly after pupation, activity rises dramatically to reach a maximum about five times the peak larval level. Maximum activity persists for a short time, and then falls sharply prior to emergence. No activity is detectable in cn, cn(3), or cn(35K). In pupae which have zero, one, two or three doses of the cn(+) allele, activity is proportional to the number of the + alleles. This provides further evidence that the cn locus contains the structural gene for kynurenine hydroxylase. Kynurenine hydroxylase is a useful gene product for studying the events of imaginal disc differentiation.


The effects of disruptive and stabilizing selection on body size in Drosophila melanogaster. II. Analysis of responses in the thorax selection lines. An analysis was made of changes in mean and variance in some thorax selection lines. The decrease of mean thorax length in the stabilizing selection lines (S) was a consequence of a directional selection component, caused by the skewness of the frequency distributions. The slight or temporary increase of the phenotypic variance and the large increase of the mean value in the disruptive selection lines with random mating (D(R)) could be attributed to differences in reproduction between small and large flies (egg production and mating success). Phenotypic variability was high in two disruptive selection lines with compulsory mating of opposite extremes (D(-)). The mechanism of the change in variability was different in these replicate lines. In D(-)-1 the change was obtained by an increase of the environmental and the nonadditive genetic components of the variance. In D(-)-2 almost exclusively an increase of additive genetic variance occurred.


The effects of disruptive and stabilizing selection on body size in Drosophila melanogaster. I. Mean values and variances. Disruptive and stabilizing selection were applied to thorax and wing length in Drosophila melanogaster. Disruptive selection with negative assortative mating (D(-)) practiced on thorax length caused a large increase of the phenotypic variance; practiced on wing length the increase was less striking. Disruptive selection with random mating (D(R)) caused in most lines only a temporary increase in phenotypic variance, but mean values increased considerably. Stabilizing selection (S) on thorax length or wing length did not decrease the phenotypic variance, but the mean value of the selected character declined.-The proportion of flies emerging decreased in all lines, while development time increased. Variance of development time increased in the D(-)-lines. In both D(-)-lines the frequency of flies with an abnormal number of scutellars was high (> 60% in one of the lines) and there was a temporary increase in abnormal segmentation of the abdomen.


Association of Bacillus cereus with food poisoning in dogs.


Changes in -S-S- linked structures of the sperm tail during epididymal maturation, with comparative observations in sub-mammalian species.


Poor transformability with novr and eryr donor DNA of some mitomycin-C-sensitive strains of Haemophilus influenzae.


The nature of chromosomal fragments in X-chromosomes of Drosophila melanogaster.


Strong antimutagenic effects of fluoride on mutation induction by trenimon and I-phenyl-3,3-dimethyltriazene in Drosophila melanogaster.


On the recovery of adhesiveness by trypsin-dissociated cells.


Putrescine metabolizing enzyme activities in some rat tissues during postnatal development.


Isometric training of rats--effects upon fast and slow muscle and modification by an anabolic hormone (nandrolone decanoate). I. Female rats.


Studies of the heterogeneity of Streptomyces griseus protease. Isolation and characterization of an alkaline serine protease from commercial pronase-P derived from Streptomyces griseus K1.


A rapid micro-assay of gentamicin, kanamycin or streptomycin in serum containing penicillins or cephalosporins.


Aspects of the pharmacology of gentamicin during the neonatal period.


Pseudomonas bacteremia. Evaluation of factors influencing response to therapy.


The kinetics and thermodynamics of death in multicellular organisms.


The microbiology of gentamicin resistance.


A new genus and species of cryptosporidiid Coccidia from India.


Role of Pseudomonas aeruginosa in actinomycosis-like bovine mastitis.


Pulmonary gas exchange following hemorrhagic shock and massive blood transfusion in the baboon.


Nonketotic hyperglycemia and epilepsia partialis continua.


Membrane leakage in Bacillus subtilis 168 induced by the hop constituents lupulone, humulone, isohumulone and humulinic acid.


Purification and properties of a carotene co-oxidizing lipoxygenase from peas.


Protocatechuate 3,4-dioxygenase. IV. Preparation and properties of apo- and reconstituted enzymes.


Purification and some properties or erythrocyte carbonic anhydrase from the European moose.


Late prognosis of severe brain injury in children.


Mechanisms of cerebrospinal fluid absorption.


Dose and time dependent suppressive and enhancing effect of cytostatics in vitro on the target cell killing activity of lymphocytes.


The kinetics and distribution of potassium in the toad bladder.


Urea transport in the toad bladder; coupling of urea flows.


Pathways for movement of ions and water across toad urinary bladder. I. Anatomic site of transepithelial shunt pathways.


Mucociliary abnormalities underlying impaired mucus elimination.


Congenitally obstructed bladders: fate after prolonged defunctionalization.


Variation in bacteriuria with intestinal loop urinary diversions.


A study of the dependence of the human erythrocyte glucose transport system on membrane sulfhydryl groups.


Stone-containing pyelocaliceal diverticulum: embryogenic, anatomic, radiologic and clinical characteristics.


The negative effects of supervoltage external irradiation in prostatic carcinoma: report of 2 cases.


Intravenous hyperalimentation: a useful adjunct in surgery of the azotemic patient.


A century of progress in experimental leprosy.


Preparation and use of malarial placenta antigen for immunodiffusion studies in a Nigerian population.


Concomitant infection of albino mice by Trypanosoma cruzi and Schistosoma mansoni. Parasitological parameters.


Studies with the schistosomicide oxamniquine (UK-4271). II. Activity in primates.


Letter: Plasma IgE levels and Schistosoma haematobium infection.


Hepatozoon-like parasite (schizonts) in the myocardium of the domestic cat.


Neuraminidase and anti-neuraminidase serum: effect on the cell surface properties.


Selective serum IgA deficiency. Frequency among 15,200 French blood donors.


Quantitative variation in the G antigen of the Rh blood group system.


Independence of the Colton and Yt blood group systems.


A gonadotropin inhibiting principle in the pineal of human fetuses. Evidence for its identity with arginine vasotocin.


Lack of effect of steroids on thyrotropin-releasing hormone (TRH) mediated thyrotropin (TSH) release in man.


Establishment of new mouse and human pigmented melanoma lines in tissue culture.


Harding-Passey melanoma in the BALB-C mouse as a model for studying the interactions between host macrophages and tumor cells.


Biochemistry of melanin synthesis: in vivo effects of MSH on tyrosinase and melanogenesis of pigmentary system.


Control of pigment synthesis in culture.


Quantitative measurement of melanin as tyrosine equivalents and as weight of purified melanin.


Regulatory mechanisms of tyrosinase activity in melanocyte. I.


A revised concept of mammalian melanogenesis: the possible synergistic functions of aerobic dopa oxidase and peroxidase. A review.


Histochemical evidence that peroxidase does not affect melanin formation in feather melanocytes.


Purification and properties of tyrosinase isoenzymes from hamster melanoma.


The tyrosinase-protyrosinase system in frog epidermis.


Peroxidase, "protyrosinase," and the multiple forms of tyrosinase in mice.


Immunological studies on malignant melanomas of man.


Hormonal control of melanocytes: MSH-sensitive adenyl cyclase in the Cloudman melanoma.


Effect of TRH (thyrotropin-releasing hormone) on the fine structure and replication of TSH and prolactin cells in the rat.


Antibacterial activity of diacetyl and its influence on the keeping quality of milk.


Diffuse lymphoma of the intestines with a monoclonal gammopathy of IgG3 kappa type.


Plasma cell interstitial pneumonia and macroglobulinemia. A response to corticosteroid and cyclophosphamide therapy.


Waldenström's macroglobulinemia in a patient with a chronic biologic false-positive serologic test for syphilis.


Candida tropicalis and Torulopsis glabrata fungemia in a patient treated with long-term hyperalimentation.


Disodium cromoglycate "Intal" (Fisons) "Rynacrom" (Fisons).


Connecticut gonorrhea screening program.


Paraproteinemias.

Paraproteinemias.


Active immunization to 17 beta-estradiol and its effects upon the reproductive cycle of the rhesus monkey.


Thyroid activation in rats after intrapituitary administration of synthetic thyrotrophin-releasing factor (TRF).


Delta 5-3 beta-hydroxysteroid dehydrogenase-delta 5-delta 4- isomerase activity in the corpus luteum of the pregnant rhesus monkey.


The effect of fetal sex on placental biosynthesis of progesterone.


Metabolic effects of oral contraceptives in monkeys fed adequate protein & low proein diets. The effects of 2 oral contraceptives, Ovulen and Norlestrin, were studied in monkeys fed adequate protein and low protein diets.  The experiment was carried out in parts.  In the first one, the administration of contraceptives was cyclic and similar to that employed in human subjects.  In the other experiments, the contraceptives were given continuously and an attempt was made to exaggerate the deleterious effects of the oral contraceptive on the liver by including small doses of a known hepatotoxic agent, aflatoxin (AT).  In Experiment 1, 45 female monkeys were divided into 2 groups of 20 and 25 and received an adequate protein (16%) and low protein diet (4%) respectively.  Each monkey was fed 1/5 of a tablet of Ovulen or Norlestrin orally for 3 weeks, and then administration was discontinued for 1 week.  In Experiment 2, 35 female monkeys were divided into 7 groups of 5 each.  All the animals recieved 4% protein diet.  5 groups were tube fed at the rate of 100 cal/kg body weight, while 2 groups were given diet  ad libitum.  Group I received the diet alone while groups II-V received 10 mcg AT, 25 mcg AT, 10 mcg AT plus 1/5 Ovulen tablets, and 25 mcg AT plus 1/5 Ovulen tablet respectively daily.  Groups VI and VII received the diet ad libitum but were orally fed 75 mcg AT and 75 mcg AT plus 1/5 Ovulen tablet respectively.  Serum glutamic-oxalacetic transaminase activity and alkaline phosphatase activity were studied at regular intervals after the administation of oral contraceptives in the experiments.  Serum proteins and hemoglobin were also determined.  Monkeys fed oral contraceptives showed increased serum glutamic-oxalacetic transaminase and alkaline phosphatase activities irrespective of the level of protein in the diet.  Livers of animals receiving oral contraceptives were morphologically similar to the controls fed respective diets.  The experiments were conducted for a period of almost 2 years.


Studies on different strains of vaccinia virus with particular reference to their quality regarding immunogenicity and reactogenicity. 3. Studies in rabbits and monkeys (Macaca mulatta).


Genital listeriosis in Delhi (India): a pilot study.


Editorial: The use of tears for heterozygote detection and genetic counseling.


Antibody to hepatitis associated antigen in blood donors of Delhi by Sandwich solid phase radioimmunoassay.


Lack of mutagenicity of the cyclamate metabolites in Drosophila.


Health insurance in Canada--an overview and commentary.


Use of a hyperbaric solution for administration of intrathecal amphotericin B.


Traumatic shock in man.

Traumatic shock in man.


Letter: Actions of propranolol in angina pectoris.


Letter: Possible Chagas's disease in United States.


Two-stage model for human enamel demineralization as determined by scanning electron microscope analysis.


Cisternograms in the primate Macaca mulatta.


Statistical relationship between the presence of human subgingival anaerobic diptheroids and periodontal disease.


Local cerebral blood flow.

Local cerebral blood flow.


Neglect after mesencephalic reticular formation lesions.


Evaluation of patients with coronary artery disease.


Specific injuries of sport.

Specific injuries of sport.


Hemobilia.

Hemobilia.


The value of laparotomy and splenectomy in the staging of 56 patients with Hodgkin's disease.


Chromosome studies in paroxysmal nocturnal haemoglobinuria.


A new unstable haemoglobin: Hb Buenos Aires, beta 85 (F1) Phe leads to Ser.


Rhesus and squirrel monkey placental specific antigen(s).


Estriol and estetrol in amniotic fluid.

Estriol and estetrol in amniotic fluid.


Present status of intrauterine diagnosis of genetic defects.


Formation of cyanide from o-chlorobenzylidene malononitrile and its toxicological significance.


Symptomatic relief of the pre-varicose syndrome and the symptomatology associated with varicose veins using ergot compounds.


Ternidens deminutus (Railliet & Henry, 1909) and hookworm in Rhodesia and a review of the treatment of human infections with T. deminutus.


Cell density as a selective parameter in tetrahymena.


Changes caused by air-drying and alcohol dehydration of spread insect sperm and fungal spore chromosome fibres.


Measurement of the potency of allergy extracts by their inhibitory capacities in the radioallergosorbent test.


Increased circulating IgE in tropical eosinophilia.


Temperature-induced derepression of tryptophan biosynthesis in a tryptophanyl-transfer ribonucleic acid synthetase mutant of Bacillus subtilis. A tryptophanyl-transfer ribonucleic acid (tRNA) synthetase (l-tryptophan: tRNA ligase adenosine monophosphate, EC 6.1.1.2) mutant (trpS1) of Bacillus subtilis is derepressed for enzymes of the tryptophan biosynthetic pathway at temperatures which reduce the growth rate but still allow exponential growth. Derepression of anthranilate synthase in a tryptophan-supplemented medium (50 mug/ml) is maximal at 36 C, and the differential rate of synthesis is 600- to 2,000-fold greater than that of the wild-type strain or trpS1 revertants. A study of the derepression pattern in the mutant and its revertants indicates that the 5-fluorotryptophan recognition site of the tryptophanyl-tRNA synthetase is an integral part of the repression mechanism. Evidence for a second locus, unlinked to the trpS1 locus, which functions in the repression of tryptophan biosynthetic enzymes is presented.


Lactic acid translocation: terminal step in glycolysis by Streptococcus faecalis. Streptococcus faecalis obtains metabolic energy chiefly from the conversion of glucose to lactic acid; the present experiments deal with the mechanism of lactic acid translocation across the cytoplasmic membrane. Efflux of [(14)C]lactate from preloaded cells was accelerated by raising the external pH, and also by the ionophores nigericin and valinomycin. These results suggest that lactate leaves the cell by an electroneutral process, presumably as lactic acid. Further evidence was obtained by studying the entry of [(14)C]lactate into nonmetabolizing cells. It appears that the membrane is essentially impermeable to the lactate anion, but allows passage of lactic acid. The most persuasive evidence is that, upon establishment of a pH gradient such that the cytoplasm was alkaline, l-[(14)C]lactate accumulated in the cells against the concentration gradient. Accumulation was transient, and dissipated in parallel with the collapse of the pH gradient. The concentration gradient attained at the peak was a function of the pH difference. Ionophores which are known to collapse a pH gradient, such as nigericin and valinomycin, abolished accumulation of l-lactate. We infer that lactic acid translocation, whether into the cells or outward, is an electroneutral process and for that reason the distribution of lactic acid across the membrane is a function of the pH of cytoplasm and medium. The specificity of translocation and its kinetic parameters suggest that it is mediated by a carrier of low specificity.


Polyamine levels during growth, sporulation, and spore germination of Bacillus megaterium. Spermidine was the major (>95%) polyamine of Bacillus megaterium in all stages of growth, although it could be replaced completely by spermine. Log-phase cells had 40 to 50% as much spermidine, based on ribonucleic acid (RNA) content, as did either stationary-phase cells or dormant spores; similar results were obtained in three other bacilli including an asporogenous mutant. Polyamine levels were essentially the same in B. megaterium grown in rich or poor media, or in media of high or low ionic strength. Polyamine levels were elevated three- to sixfold by exogenous spermidine without a major effect on growth, sporulation, or subsequent spore germination. During germination, the absolute amount of spermidine remained constant for almost 2 h until net RNA synthesis had lowered the polyamine/RNA ratio to a value close to that in log-phase cells. At this time, the spermidine level began to rise, and thereafter spermidine and RNA increased in parallel. This parallel relationship between the spermidine and RNA levels was abolished by actinomycin D, but not by chloramphenicol.