Treatment for hypertension.

Treatment for hypertension.


Quantitative uptake measurements with a shadow shield whole-body counter.


Intrinsic dwarfism presenting at birth. Preliminary communication on nomenclature.


Comparison of carbenicillin and penicillin in the treatment of uncomplicated gonorrhea.


Current concepts in therapy: general concepts in the use of antibiotics in adults.


Abortion associated with Brucella abortus (Biotype 1) in the T.B. mare.


Autoimmune hemolytic anemia caused by anti-D.


Post-Konyne hepatitis: the ineffectiveness of screening for the hepatitis B antigen (HBAg).


Nonspecific warm hemolysins of papain-treated cells: serologic characterization and transfusion risk.


Another pitfall in blood group testing for nonpaternity.


Interrelations between diagnostic criteria of the iron status and 50-mg ferrous iron absorption in iron-replete and iron-deficient subjects.


The histochemistry of fibrinolysis in haemophilic synovial membranes.


Ready release of intracellular muramidase (lysozyme) from mononuclear cells in the skin window exudates.


Myeloma in mother and daughter.

Myeloma in mother and daughter.


Recombination experiments with an unusual L-chain related myeloma protein.


Myelofibrosis, osteolytic bone lesions and hypercalcemia in chronic myeloid leukemia.


Nitroglycerin and coronary blood flow in coronary artery disease.


Use of parenteral nutrition in treatment of advanced regional enteritis.


Paresthesias and hypophosphatemia occurring with parenteral alimentation.


Hormone and enzyme levels in normal and complicated pregnancy.


Localization of human chorionic gonadotropic and somatomammotropic hormones by the peroxidase immunohistoenzymologic method in villi and amniotic epithelium of human placentas (from six weeks to term).


Scrub typhus in South Vietnam. A study of 87 cases.


Respiratory abnormalities in workers exposed to grain dust.


Chronic exposure to nitrogen dioxide.

Chronic exposure to nitrogen dioxide.


Pressure effect on ERG and optic nerve conduction of visual impulse. Short-term effects in owl monkeys.


Unusual intranuclear tubular structures associated with the maturation of Herpesvirus saimiri in monkey kidney cell cultures. Unusual intranuclear tubules have been observed in cultures of both African green monkey and owl monkey kidney cells infected with Herpesvirus saimiri; the material was studied in thin sections with the electron microscope. The tubules were seen in about 10% of virus-containing cells at the stage when the virus matured by budding at the nuclear membrane, measured 160-180 nm in diameter and up to 3.6 μm in length, were bounded by an outer "membrane" and contained beneath this an electron dense repeating structure arranged either as a coil or a series of evenly spaced rings. The morphology and significance of the tubules are discussed.


Kinetics of entry and distribution of 5-fluorouracil in cerebrospinal fluid and brain following intravenous injection in a primate.


Increase of soluble RNA methylase activities by chemical carcinogens.


Immunological progress in bilharziasis.

Immunological progress in bilharziasis.


The translabyrinthine removal of acoustic and other cerebellopontine angle tumors.


Electrical stimulation of the inner ear.


Infective endocarditis in drug addicts. Experiences with medical and surgical treatment.


Evaluation of hollow fiber and spiral coil membrane oxygenators designed for cardiopulmonary bypass in infants.


Studies of the distribution of a new avian pancreatic polypeptide and insulin among birds, reptiles, amphibians and mammals.


Morphological and functional evidence for corpus luteum activity during late pregnancy in the rhesus monkey.


The biological response of infant nonhuman primates to a polychlorinated biphenyl.


Toxicity of metabolites produced by the "Alternaria".


Protein calorie malnutrition in early childhood.


Diseases from pets.

Diseases from pets.


Contributions of army medicine to civilian medicine.


Red cell density in various blood disorders.


A study of malaria rates in the Que Son Mountains of Vietnam.


Case for diagnosis.

Case for diagnosis.


Prophylaxis of malaria in man using the sulfone DFD with pyrimethamine.


A survey to assess potential human disease hazards along proposed sea level canal routes in Panamà and Colombia. IV. Arbovirus surveillance in man.


Tuberculosis in active duty military personnel.


Clinical decisions and outcomes in a navy psychiatric service.


The military pediatric experience as training.


In vitro thyroid function tests: a comparison of some commercially available kits.


Gonorrhea in a military prenatal population.


Beta-hemolytic streptococcus in a population of antepartum patients.


Lennox's syndrome and lead intoxication.


Gonococcal arthritis in a newborn infant.


Gonococcal tonsillitis-pharyngitis in a 5-year-old girl.


Pesticides in soil: organochlorine pesticide residues in soils and crops of the Corn Belt region, United States--1970.


An interracial study in the management of infants and children with convulsions.


A technique for chronic subclavian vein catheterization.


Pesticide residues in food.

Pesticide residues in food.


Aggression within social groups of rhesus monkeys (Macaca mulatta): effect of contact between groups.


Monkeys (Macaca mulatta) raised only with peers. A pilot study.


Courtship processing in Drosophila melanogaster. I. Selection for receptivity to wingless males.


Courtship processing in Drosophila melanogaster. II. An adaptation to selection for receptivity to wingless males.


Australia antigen in Zaïre. Studies on leprosy.


Additional phage-typing scheme for C. diphtheriae.


A lattice structure in water-swollen liver mitochondria.


Iodoacetamide-induced sensitization of the pancreatic beta-cells to glucose stimulation. At a glucose concentration of 3mm or less, iodoacetamide had no effect on the release of insulin from microdissected pancreatic islets of ob/ob-mice. At higher glucose concentrations, iodoacetamide exerted both an initial stimulatory and a subsequent inhibitory action. When islets were perifused with 1mm-iodoacetamide and 17mm-glucose the inhibitory action predominated after about 15min of transient stimulation. With decreasing concentrations of iodoacetamide the stimulatory phase was gradually prolonged, and with 0.003-0.1mm-iodoacetamide stimulation only was observed for 75min. Prolonged stimulation was also noted after a short pulse of iodoacetamide. Similar responses to 0.1mm-iodoacetamide were observed with islets from normal mice. With islets from ob/ob-mice the effect of 0.1mm-iodoacetamide was reproduced with 0.1mm-iodoacetate, whereas 0.1mm-acetamide had no apparent effect. Iodoacetamide increased the V(max.) of glucose-stimulated insulin release without altering the apparent K(m) for glucose. Leucine, glibenclamide or theophylline could not replace glucose in this synergistic action with iodoacetamide. Iodoacetamide rather inhibited the insulin-releasing action of theophylline. Iodoacetamide-induced potentiation of the glucose-stimulated insulin release was rapidly and reversibly inhibited by mannoheptulose, adrenaline, or calcium deficiency. The potentiating effect on insulin release was not paralleled by effects on glucose oxidation or on islet fructose 1,6-diphosphate. However, the inhibitory action of iodoacetamide might be explained by inhibition of glycolysis as evidenced by an inhibition of glucose oxidation and a rise of fructose 1,6-diphosphate. The results support our previous hypothesis that thiol reagents can stimulate insulin release by acting on relatively superficial thiol groups in the beta-cell plasma membrane. Glycolysis seems to be necessary in order for iodoacetamide to stimulate in this way.


Bioavailability of peroral dosage forms.


The purification and properties of extracellular glycosidases of the cellular slime mould Dictyostelium discoideum. The purification of beta-N-acetylhexosaminidase, alpha-glucosidase, alpha-mannosidase and beta-glucosidase from the spent growth medium of Dictyostelium discoideum strain Ax-2 myxamoebae is described. beta-N-Acetylhexosaminidase and alpha-glucosidase were obtained in high yield and as homogeneous preparations whereas the alpha-mannosidase preparation consisted of two electrophoretically distinct isoenzymes. The physical, chemical and kinetic properties of these enzymes are described.


Anatomical differences between the neocortex of man and other primates.


Centrifugal fibers to the retina in the rhesus monkey.


Enteral hyperalimentation with elemental diet.


Local metabolic changes in spinal cord injury.


Double-antibody solid-phase radioimmunoassays for gonadotropins in serum.


TRF test in patients with hypothalamic-pituitary disorders.


Resynthesis of chick blastodermic cells following dissociation.


The jejunal mucosa in protein-energy malnutrition. A clinical, histological, and ultrastructural study.


Studies of the use of tumor vaccine for the cancer patient.


Production of anti phenobarbital antibody in rabbits by immunization with p-azophenobarbital acetylated bovine serum albumin.


Pathophysiology of shock secondary to underwater decompression sickness.


Circular dichroism studies on protohemoproteins. 3. cytochrome b5 from rabbit liver microsomes, and cytochrome b-557.5 from Rhodospirillum rubrum.


The naso-temporal division of the monkey's retina.


Pseudomonas pneumonia.

Pseudomonas pneumonia.


A new small diameter mass spectrometer cannula for medical use.


Intermediate care for the aged: accommodation requirements and economic considerations.


Urinary tract infection and secretory urinary IgA in malnutrition.


Some observations on pelvic floor evolution in primates.


Absence of nerve growth factor in the venoms of bees, scorpions, spiders and toads.


Waldenström's macroglobulinaemia with xanthomatosis and hypercholesterolaemia. Report of a case.


The strategy of identification of mycobacteria in a regional reference laboratory.


Identification of mycobacteria as a clinical service.


Pathogenicity as the main differentiating character within the avium-intracellulare complex.


Study of mycobacteria strains belonging to the avian-intracellular group isolated from monkeys.


Immunodiffusion analysis--a rational basis for the taxonomy of mycobacteria.


Fractionation and purification of the antigenic material for immunogenic studies of atypical mycobacteria.


Thin-layer chromatography of mycobacterial lipids as an aid to classification.


Intra-specific variation in the mycobacteria. A taxonomic aid.


Ectopic bone formation in patients with spinal cord injury.


Some properties of the uridine diphosphate glucuronyltransferase activity synthesizing thio-beta-D-glucuronides. 1. Some properties of the UDP-glucuronyltransferase synthesizing thio-beta-d-glucuronides were investigated and compared with those of the enzyme synthesizing the O-glucuronides of analogous phenols. 2. Enzyme activity was generally similar for both classes of substrate in tissue distribution, intracellular location, optimum pH, perinatal development and induction by organ culture or by phenobarbital. 3. Certain differences were noted between the two types of activity in behaviour on storage and on activation, in kinetic behaviour and in distribution between Wistar and Gunn rats; the Gunn rats were not deficient in hepatic UDP-glucuronyltransferase activity towards o-aminothiophenol. 4. These differences are no greater than those exhibited in the synthesis of various O-glucuronides; therefore thiolic substrates could compete in vivo with phenolic compounds for access to the UDP-glucuronyltransferase complex as well as for UDP-glucuronic acid.


The characterization of C-phycocyanin from an extremely halo-tolerant blue-green alga, Coccochloris elabens. C-Phycocyanin was isolated and purified from a uni-algal culture of an extremely halo-tolerant blue-green alga, Coccochloris elabens. This alga can be grown under laboratory conditions in 25% (w/v) NaCl. Purified halophile phycocyanin was characterized by amino acid analysis and the measurement of sedimentation velocity, fluorescence polarization and immunodiffusion as a function of protein concentration, pH and ionic strength. The results were compared with those of studies of phycocyanin isolated from Plectonema calothricoides and from several other sources. The states of aggregation previously characterized as being present in other C-phycocyanins, monomer, trimer and hexamer, were present in halophile phycocyanin and were characterized as antigenically related to all C-phycocyanins tested. The equilibrium between 3S monomer and 11S hexamer at low concentrations in halophile phycocyanin was quantitatively similar to that for other phycocyanins. The effect of pH and ionic strength on the 6S (trimer) and 11S (hexamer) aggregation of halophile phycocyanin was markedly salt-dependent and the relative amount of each aggregate in the presence of 2m-NaCl was like that of C-phycocyanin from mesophiles, in the absence of additional salt. In antigenic relationship and aggregation properties, the phycocyanin from C. elabens appeared to be most closely related to that isolated from the thermophilic blue-green alga, Synechococcus lividus. Amino acid content of the halophile phycocyanin indicated the presence of a significantly larger number of acidic residues than that found in mesophiles. Explanations of the properties of the halophile protein require consideration of a strong contribution of hydrophobic forces and utilize both charge-shielding and salting-out effects.


The organ-specificity of ferritin in human and horse liver and spleen. 1. Ferritin was isolated from human and horse spleen and liver, and apoferritin prepared therefrom. 2. The electrophoretic mobilities of the four apoferritins were determined on polyacrylamide gels and on cellulose acetate strips, and all found to be equal. 3. Homologous ferritins share reactions of identity in immunodiffusion experiments, whereas heterologous ferritins show only partial identity. 4. The subunit molecular weight of each of the apoferritins was determined by polyacrylamide-gel electrophoresis in sodium dodecyl sulphate and by chromatography on agarose columns in 6m-guanidine-HCl. A value of approx. 18500 was found in all cases. The proteins all had sedimentation coefficients of 17-18S. It thus seems that they have identical quaternary structures. 5. The amino acid compositions of the proteins revealed distinct differences both between organs and between species. This was confirmed by analysis of the tryptic peptide patterns, where it was found that about one-third of the peptides were common to the four proteins and the other two-thirds varied from protein to protein. 6. It is concluded that the apoferritins present in the liver and spleen of human and horse are both organ- and species-specific. 7. The apoferritin isolated from the liver of a patient with idiopathic haemochromatosis was identical with normal human liver apoferritin by the criteria described above.


Immunological properties of N-acetyl-beta-D-glucosaminidase of normal human liver and of GM2-gangliosidosis liver. Antisera were raised to a partially purified preparation of human liver hexosaminidase and to highly purified preparations of hexosaminidase isoenzymes A and B. All the antisera precipitated the enzyme in an enzymically active form, which could be located on immunodiffusion and immunoelectrophoretic gels by using a histochemical substrate. The antisera to the purified isoenzymes were shown to react with hexosaminidase from human liver, kidney, brain and spleen, but did not cross-react with human liver beta-glucosidase, beta-galactosidase, alpha-mannosidase, beta-xylosidase, arylsulphatase or acid phosphatase. Hexosaminidases A and B were immunologically identical. The immunological properties of the hexosaminidases from livers of patients with three types of GM(2)-gangliosidoses were closely similar. No evidence could be found for cross-reacting material in enzyme-deficient states.


The inhibitory effect of manganese on transmitter release at the neuromuscular junction of the toad. 1. Manganese, at low concentrations (0.5-2.0 mM), blocks neuromuscular transmission in toads. Endplate potentials (e.p.ps) are reduced in amplitude but the amplitude of miniature endplate potentials (m.e.p.ps) is, if anything, increased.2. The release of transmitter by an action potential is reduced in solutions containing Mn, but is still well described by the Poisson equation.3. Log-log plots of e.p.p. quantal content (m) against [Ca] have a mean gradient of 3.73, and a model based on the co-operative action of four calcium ions in excitation-secretion coupling, and competitive inhibition by Mn, was constructed. The model, with its exponent of 4, is shown in fact to predict gradients of less than four for log-log plots of m against [Ca].4. The assumption of competitive inhibition by Mn was supported by modified Lineweaver-Burk plots of m(-(1/4)) against 1/[Ca]. The mean dissociation constants for Ca and Mn were 1.3 mM and 0.15 mM respectively. From the model, an upper estimate of the density of calcium ;receptor sites' of 30 per square micron of nerve terminal was obtained.5. In contrast to its inhibitory effect on evoked release, Mn increases the spontaneous release of transmitter. It is suggested that Mn may increase m.e.p.p. frequency by releasing Ca from an intracellular store.


Glial fibrillary acidic protein from normal human brain. Purification and properties.


Influence of sleep-waking and nociceptor-induced behavior on the activity of supraoptic neurons in the hypothalamus of the monkey.


Two cases of Hunter's syndrome--the anaesthetic and operative difficulties in oral surgery.


Osmosensitivity of hypothalamic magnocellular neuroendocrine cells to intracarotid hypertonic D-glucose in the waking monkey.


On perivascular astrocytic membrane specializations in monkey optic nerve.


Supraependymal cells: light and transmission electron microscopy extends scanning electron microscopic demonstration.


A clinical and microbiological study of venous catheterization. In a prospective study of 210 intravenous catheters and needles 31 (15%) swabs yielded 34 microbial isolates. Twenty-eight were "non-pathogenic" skin commensals and six were "pathogenic" organisms. Commensals were isolated predominantly from catheters left in situ for only a short time (up to two days) and pathogenic organisms most frequently from specimens left in situ for a longer time. Correlation was not confirmed between colonization and infection, antibiotic therapy or phlebitis. All samples of IV fluids were sterile on culture. Changing the IV catheter at least every two days is recommended.


Pediatric antimicrobial therapy. II.

Pediatric antimicrobial therapy. II.


Listeria monocytogenes infections in Canada. Between 1951 and January 1972 listeriosis was diagnosed bacteriologically in 101 Canadian patients. This study adds 80 cases to the 21 reported from Metropolitan Toronto by Sepp and Roy in 1963. The Laboratory Centre for Disease Control, Ottawa, collated epidemiological and clinical data. Serotypes of Listeria monocytogenes included 4b (53), 1 (15), 1b (6), 1a (2), 2 and 3. Clinically, 54 patients had meningitis and 23 septicemia. The mortality rate was 30%.Between 1954 and January 1972 listeriosis affected 15 British Columbian patients: nine were male and six female; 12 were less than 1 or more than 45 years old. Among the patients were a pregnant mother and the son to whom she gave premature birth. A day-old infant and an elderly man died.


Molecular interactions in the phenomenology of the onset of mental illness.


Radiosensitivity of oocytes of Drosophila. 3. Sensitivity of class-B oocytes of XX-O and X-X females.


Effect of mycorrhizal infection on nitrogen and phosphorus nutrition of ericaceous plants.


Incidence of signs and symptoms in thyroid disease.


Tetracycline interference with the bactericidal effect of serum.


Effect of centrifugation on cell adhesion.


Serum transferrin levels and the assessment of protein nutritional status.


Effects of tiger snake venom on the ultrastructure of motor nerve terminals.


A method for obtaining biochemical and biophysical measurements from rhesus monkey fetuses for prolonged periods.


Relationship between 3H-ryanodine uptake and myocardial contractility.


Avian thrombocyte aggregation and shape change in vitro.


Immune hemolytic transfusion reactions in monkeys: activation of the kallikrein system.


Specific antilymphocyte antibody for use in renal transplantation.


A constant bile duct anomaly encountered during biliary operation.


Long term treatment of diabetes mellitus with glibornuride. A new hypoglycemic agent.


Malaria studies in vitro. II. The measurement of drug activities using leucocyte-free blood-dilution cultures of Plasmodium berghei and 3H-leucine.


The isolation from human parotid saliva and partial characterization of the protein core of a major parotid glycoprotein.


Autonomic innervation of the salivary glands in the marmoset (Callithrix penicillata Jordani); with some gross anatomical and histological observations.


Studies on the interaction between rabbit liver pyruvate kinase and its allosteric effector fructose 1,6-diphosphate. Preparation of the L form of rabbit liver pyruvate kinase (EC 2.7.1.40) in the presence of fructose 1,6-diphosphate yielded an enzyme which was kinetically identical with the M or muscle-type form of pyruvate kinase found in liver. Chromatographic and dialysis studies of this complex showed that most of the fructose 1,6-diphosphate molecules were loosely bound to the enzyme, but dilution-dissociation studies and binding experiments established that there was a high initial affinity between the enzyme and fructose 1,6-diphosphate (K(assoc.)=2.3x10(9)), and that binding of the loosely bound fructose 1,6-diphosphate was concentration-dependent and a necessary condition to overcome the co-operative interaction observed with the homotropic effector phosphoenolpyruvate. Preparation of the liver enzyme in the absence of EDTA did not yield a predominantly M form of the enzyme, and incubation of the M form in the presence of EDTA did not convert it into the L form, but resulted in inhibition of enzyme activity. Immunological studies confirmed that the L and M forms in liver were distinct, and that preparation of the L form in the presence of fructose 1,6-diphosphate did not produce an enzyme antigenically different from the L form prepared in the absence of this heterotropic effector.


Investigations of the structural function of the J chain in human immunoglobulin M. Human IgM molecules were treated with Na(2)SO(3) or mercaptoethylamine in concentrations ranging from 2 to 14mm or 2 to 22mm respectively. The dissociation of IgM to IgM(s) varied from 0% to 100%. At the intermediate concentrations of either reagent the amount of freed J chains was less than expected. In an attempt to find an explanation for this, IgM was partially dissociated to IgM(s) with mercaptoethylamine. The IgM(s) isolated by gel filtration was divided according to the ascending and descending portions of the elution curve. These portions were treated with 24mm-mercaptoethylamine and analysed for the presence of J chains. Only the ascending portion contained free J chains. Thus, after mild reduction where not all the IgM molecules are dissociated to IgM(s), some J chains remain covalently attached to some IgM(s) molecules although most of the J chains are freed. It was concluded that the J chain could serve as a ;hitch' for IgM(s) molecules forming intact IgM.


Inhibition by alpha-macroglobulin and other serum proteins. 1. Normal human serum was found to inhibit human cathepsin B1. 2. The major inhibitor present in serum was purified and identified as alpha(2)-macroglobulin. 3. alpha(2)-Macroglobulin was found to bind cathepsin B1 in an approximately 1:1 molar ratio. When bound, the enzyme retained about 50% of its proteolytic activity, and up to 80% of its activity against alpha-N-benzoyl-dl-arginine 2-naphthylamide. 4. Pretreatment of alpha(2)-macroglobulin with cathepsin B1 inactivated by exposure to pH8.5 or iodoacetic acid, in large molar excess, did not prevent the subsequent binding of active enzyme. Active enzyme, once bound, was not protected from inhibition by 1-chloro-4-phenyl-3-tosylamido-l-butan-2-one. 5. Cathepsin B1 was also inhibited by human immunoglobulin G, at high concentration. 6. Because it had been suggested that haptoglobin is responsible for the inhibition of ;cathepsin B' by serum, a method was devised for the selective removal of haptoglobin from mixtures of serum proteins by adsorption on haemoglobin covalently linked to Sepharose. No evidence was obtained that haptoglobin has any inhibitory activity against the enzyme.


Purification and characterization of two fructose diphosphate aldolases from Escherichia coli (Crookes' strain). Two fructose diphosphate aldolases (EC 4.1.2.13) were detected in extracts of Escherichia coli (Crookes' strain) grown on pyruvate or lactate. The two enzymes can be resolved by chromatography on DEAE-cellulose at pH7.5, or by gel filtration on Sephadex G-200, and both have been obtained in a pure state. One is a typical bacterial aldolase (class II) in that it is strongly inhibited by metal-chelating agents and is reactivated by bivalent metal ions, e.g. Ca(2+), Zn(2+). It is a dimer with a molecular weight of approx. 70000, and the K(m) value for fructose diphosphate is about 0.85mm. The other aldolase is not dependent on metal ions for its activity, but is inhibited by reduction with NaBH(4) in the presence of substrate. The K(m) value for fructose diphosphate is about 20mum (although the Lineweaver-Burk plot is not linear) and the enzyme is probably a tetramer with molecular weight approx. 140000. It has been crystallized. On the basis of these properties it is tentatively assigned to class I. The appearance of a class I aldolase in bacteria was unexpected, and its synthesis in E. coli is apparently favoured by conditions of gluconeogenesis. Only aldolase of class II was found in E. coli that had been grown on glucose. The significance of these results for the evolution of fructose diphosphate aldolases is briefly discussed.


Species variations in the O-methylation of n-butyl 4-hydroxy-3,5-diiodobenzoate.


Radiographic studies of amyloidosis.

Radiographic studies of amyloidosis.


Plasmodium spp.: dispersion of malarial oocyst populations in anopheline and culicine mosquitoes.


Humoral antibody response and Ig characterization of the specific agglutinins in rabbits during experimental American trypanosomiasis.


Computerized microacidimetric determination of beta lactamase Michaelis-Menten constants.


Binding of thyroid hormones to a soluble fraction from porcine anterior pituitary.


Ultrastructural development of Nostoc muscorum A.


Growth of pseudomonas species on phenylacetamide.


Unbalanced growth and replication of chloroplast populations in Euglena gracilis.


Inactivation of southern bean mosaic virus and its ribonucleic acid by nitrous acid and ultraviolet light.


Superficial venous arrangement of the face and neck of the tufted capuchin, Cebus apella Linnaeus, 1758.


The isolation of tyrosinase from Aspergillus nidulans, its kinetic and molecular properties and some consideration of its activity in vivo.


Studies on human malaria in Aotus monkeys. I. Sporozoite transmission of Plasmodium vivax from El Salvador.


Studies on human malaria in Aotus monkeys. II. Establishment of a strain of Plasmodium falciparum from Panama.


The 2-mercaptoethanol labile immunoglobulin response of beagles experimentally infected with Dirofilaria immitis.


Demodectic mites of subhuman primates I: Demodex saimiri sp. n. (Acari: Demodicidae) from the squirrel monkey, Saimiri sciureus.


A simple method for the detection of human congenital Chagas' disease.


Ultrastructural changes in Babesia argentina-infected erythrocytes in kidney capillaries.


Haemogregarina platessae Lebailly (=H. achiri Saunders) from the Hogchoker in Maryland.


The sources of hospital infection.

The sources of hospital infection.


Immunity to brucella infection.

Immunity to brucella infection.


East Coast fever: correlation between the morphology and infectivity of Theileria parva developing in its tick vector.


Sound spectral properties of conditioned vocalization in monkeys.


Biological versatility and earth history. Examples from various plant and animal groups indicate that there has been a general increase in potential versatility of form, determined by the number and range of independently varying morphogenetic parameters, among taxa appearing at successively younger stages in the fossil record. Taxa or body plans with higher potential versatility have tended to replace less potentially versatile groups in the same or similar adaptive zone through time. Greater potential diversity allows for greater homeostasis, efficiency, and integration of structures and functions, and for an increase in size of the potential adaptive zone. In contrast, chemical versatility has generally decreased within groups from the pre-Cambrian to the Phanerozoic, partly as the result of apparent changes in the chemical environment and partly as the consequence of selection for efficiency and greater metabolic ease of handling of certain materials.


Carbon dioxide hydration activity of carbonic anhydrase: paradoxical consequences of the unusually rapid catalysis. The kinetic parameters for carbon dioxide hydration catalysis by carbonic anhydrase (EC 4.2.1.1) present an apparent paradox. The assumption of H(2)CO(3) as the hydration product requires the rate of recombination of H(2)CO(3) with enzyme to be faster than the diffusion limit. The alternative assumption of HCO(3) (-) as the product of hydration likewise requires active-site ionization rates to exceed the diffusion limit. We previously postulated the presence of special means for rapid active-site ionization. It is shown here that when proton transfer between enzyme and buffer species is taken into account, there is no need to invoke rates exceeding the diffusion limit. Bicarbonate ion thus appears as the most probable hydration product and dehydration substrate.


Crossing-over between X AND Y chromosomes during ribosomal DNA magnification in Drosophila melanogaster. In genetic combinations undergoing ribosomal DNA magnification, the frequency of crossing-over between X and Y chromosomes is increased at least 20-fold above that in similar combinations not undergoing magnification. Such recombination occurs at the ribosomal DNA level. The data are consistent with a model where extra copies of ribosomal DNA are formed which, after circularization, are integrated into the chromosome.


The mental hospital as an institution.

The mental hospital as an institution.


Segregation during ascidian embryogenesis of egg cytoplasmic information for tissue-specific enzyme development. Cleavage-arrested embryos of the ascidian Ciona intestinalis were able to differentiate two tissue-specific enzymes-muscle acetylcholinesterase (EC 3.1.1.7) and brain pigment cell tyrosinase (EC 1.10.3.1). Cytochalasin B, colchicine, Colcemid, and podophyllotoxin were used as cleavage inhibitors at early embryonic stages up to the 64-cell stage. Only certain cells in the cleavage-arrested embryos developed these histochemically detectable enzymes, and this ability followed the cell lineage patterns for the two tissues. This result implies the presence of specific positional information in the egg cytoplasm that is differentially segregated during cleavage. There were distinct and separate puromycin and actinomycin D sensitivity periods for the occurrence of each enzyme during development of both normal and cleavage-arrested embryos. The segregated information is apparently neither the enzyme proteins nor RNA templates for enzyme synthesis, but is probably concerned with activation of appropriate genes.


The influence of fluid shear on the kinetics of blood coagulation reactions.


The impact of waterborne diseases on mortality.


Bio-artificial liver: a combined system of hemodialysis and baboon liver perfusion.


Validation and bioengineering aspects of an implantable glucose sensor.


Economic class and use of physician services.


Morphologic studies of dialysis membranes adherence of blood components to air rinsed coils.


Membrane lung oxygenation for temporary support of the failing transplanted lung.


An outbreak of anthrax at the Chester Zoological Gardens.


The surface bonded, covalently crosslinked urokinase synthetic surface. In vitro and chronic in vivo studies.


Immunological relationship between border disease, mucosal disease and swine fever.


Human and macaque blue cones studied with electroretinography.


Persistent mixed field polyagglutinability. An immunofluorescence study in genetically abnormal red cells.


Secondary stimulation of Rh antibodies by kidney allografts from Rh-positive donors.


A new blood group antigen, Hov.

A new blood group antigen, Hov.


Rhesus blood group notations.

Rhesus blood group notations.


A service-integrated approach to clinical pharmacy practice through a hyperalimentation program.


Pseudomonas pneumonia. A retrospective study of 36 cases.


Antimicrobial activity of tobramycin and gentamicin used in combination with cephalothin and carbenicillin.


Carbon monoxide inhalation effects following myocardial infarction in monkeys.


Antitrypsin deficiency and abnormal protease inhibitor phenotypes.


Comparison of antigenicity of rat collagen prepared by various methods.


Measles giant cell pneumonia in an adult following long-term chemotherapy.


Pollutant burdens and biological response.


Studies on the epizootiology of bovine babesiosis in north eastern New South Wales.


Studies on the epizootiology of bovine babesiosis in common border areas of New South Wales and Queensland.


Inhibition of prostaglandin biosynthesis by 7-oxa- and 5-oxa-prostaglandin analogues. Some oxaprostaglandin derivatives have been shown to inhibit prostaglandin biosynthesis from arachidonate by a particulate prostaglandin synthetase preparation. The most potent inhibitor was 5-oxaprost-13-trans-enoate, and inhibition by this compound appeared to be competitive. Certain structure-activity relationships were ascertained.


Lung function after mantle field irradiation in Hodgkin's disease.


Role of radiotherapy in the treatment of cancer of the tongue. A retrospective analysis on TNM-staged tumors treated between 1958 and 1968.


Peripheral nerve tumors with rhabdomyosarcomatous differentiation (malignant "Triton" tumors).


Regional blood flows in unanaesthetized rhesus monkeys during myocardial infarction.


Ankle arthrography in acute sprains.

Ankle arthrography in acute sprains.


Effects of immune environments upon bone in vitro. I. Cytotoxicity of antibodies to bone homogenates.


Experimental scoliosis.

Experimental scoliosis.


A novel monoquaternary neuromuscular blocking agent.


Constant oxygen enhancement ratio during the mitotic cell cycle in x-rayed Drosophila melanogaster embryos.


The use of antibiotics in hospital.

The use of antibiotics in hospital.


Studies on rabbit secretory and serum antibodies to hog intrinsic factor.


Correlation between PaCO2 and regional cerebral blood volume by x-ray fluorescence.


The use of polyacrylamide gel electrophoresis in taxonomy of Brucella.


The properties of virginiamycin-resistant mutants of Bacillus subtilis.


Subunit structure of cholera toxin.

Subunit structure of cholera toxin.


Penicillin production by lysine auxotrophs of Penicillium chrysogenum.


A bacitracin-negative mutant of Bacillus licheniformis which is able to sporulate.


Mutants of Pseudomonas aeruginosa with impaired  -lactamase inducibility and increased sensitivity to  -lactam antibiotics.


Cloxacillin and nafcillin: serum binding and its relationship to antibacterial effect in mice.


Nutritional profiles of Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica in chemically defined media and the use of growth requirements for gonococcal typing.


The silvered leaf-monkey of Malaysia, Presbytis cristatus: disease model for human scrub typhus.


Experimental gingivitis in Macaca speciosa monkeys: clinical, bacteriologic and histologic similarities to human gingivitis.


Posttraumatic transient cerebral blindness. Association with migraine and seizure diatheses.


Serological typing of Pseudomonas aerginosa from mouse breeding colonies.


Changes in serotype of Pseudomonas aeruginosa.


Passive hemagglutination reaction using formalinized sheep erythrocytes treated with tannin and coated with protein moiety of the endotoxin (OEP) of Pseudomonas aeruginosa.


Micellar structure and endotoxic activities of gram-negative lipopolysaccharides.


Characterization of anti-cord factor antibody.


Regulation of staphylococcal enterotoxin B synthesis and its relation to other extracellular proteins.


Some properties of the diphtheria toxin fragment produced by a beta phage-mutant lysogen of Corynebacterium diphtheriae.


Methylation of basic residues in structural proteins.


Serial reversal learning by monkeys with inferotemporal or foveal prestriate lesions.


Diurnal variation of ileum motility in monkey during different feeding and avoidance-conditioning schedules.


Hypothalamic "coding" in the unanesthetized monkey of noradrenergic sites mediating feeding and thermoregulation.


Brain mechanisms in movement.

Brain mechanisms in movement.


Premotor cortex and instrumental behavior in monkeys.


Effect of the bee venom and melittin on plasma cortisol in the unanesthetized monkey.


Absorption from mucosal surfaces.

Absorption from mucosal surfaces.


The stimulation of eccrine sweating by pharmacologic agents.


When to use two or more antibiotics.

When to use two or more antibiotics.


Penicillin phenoxymethyl. Use in rheumatic fever prophylaxis.


Stickler syndrome in a pedigree of Pierre Robin syndrome.


Estrogenic effect in vaginal smears in cases of carcinoma in situ and microinvasive carcinoma of the uterine cervix.


Non-atheromatous stenosis and occlusion of the internal carotid artery and its main branches.


Angiographic evidence for cerebral (opercular) swelling in patients with seizures.


Tuberculoma of the brain (revisited).

Tuberculoma of the brain (revisited).


Comparison of precipitation malarial antibodies produced by indigenous immune Gambians and an infected European.


Beta-ketothiolase from Hydrogenomonas eutropha H16 and its significance in the regulation of poly-beta-hydroxybutyrate metabolism. 1. beta-Ketothiolase was purified 49-fold from fructose-grown cells of Hydrogenomonas eutropha H16 with a yield of 27%; the purification procedure involved precipitation by cetyltrimethylammonium bromide, DEAE-cellulose chromatography and exclusion chromatography on Sephadex G-200; the freeze-dried enzyme is stable. The molecular weight determined by sucrose-gradient centrifugation (8.2S) and by gel filtration is 147000-150000. The optimum pH for the cleavage reaction is 8.1, that for the condensation reaction 7.8, both measured in Tris-HCl buffer. 2. The kinetics of the cleavage reaction are described. Substrate-saturation curves were measured with both acetoacetyl-CoA and CoA as the variable substrates. The concentration of the second substrate was kept constant and was varied during successive experiments. The cleavage reaction is characterized by substrate inhibition by acetoacetyl-CoA, which is partially relieved by free CoA. Hill plots indicate two acetoacetyl-CoA-binding sites. 3. The substrate(acetyl-CoA)-saturation curve for the condensation reaction is hyperbolic. The K(m) was 3.9x10(-4)m-acetyl-CoA. In the presence of CoA sigmoidal curves were obtained, with an increasing sigmoidicity from 0.03 to 0.30mm-CoA. The inhibitory action of CoA on the beta-ketothiolase condensation reaction and its possible involvement in the regulation of poly-beta-hydroxybutyrate synthesis and degradation are discussed.


Lethal effect of rifampicin in Bacillus subtilis as a complicating factor in the assessment of the lifetime of messenger ribonucleic acid. The bactericidal action of rifampicin was compared with that of chloramphenicol in growing and in sporulating cultures of Bacillus subtilis 168. Chloramphenicol kills cells only very slowly, but exposure to rifampicin kills over 95% of cells in a few minutes, causing gross physical damage, which is visible in both phase-contrast and electron microscopy. This is accompanied by a fall in O(2) consumption and by lysis. Experiments with synchronized cultures showed that susceptibility to the lethal effect of rifampicin is greater when the cells are dividing. The results suggest that the synthesis of some species of RNA other than mRNA may be necessary for the maintenance of cell integrity, although experiments with actinomycin D do not altogether fit this interpretation. However, we conclude that rifampicin is too toxic to use as an antibiotic for assessing the lifetime of mRNA.


Properties of tRNAPhe from Drosophila.

Properties of tRNAPhe from Drosophila.


Control of the production of exo-beta-N-acetylglucosaminidase by Bacillus subtilis B. 1. The control of exo-beta-N-acetylglucosaminidase (EC 3.2.1.30) production by Bacillus subtilis B growing on a chemically defined medium was studied. 2. The enzyme was repressed during exponential growth by those carbon sources that enter the glycolytic pathway above the level of phosphoenolpyruvate. When exponential growth ceased as a result of low concentrations of the nitrogen, carbon or metal ion components of the medium, the enzyme was formed and its amount could be increased by the addition of cell-wall fragments as inducer. 3. The enzyme was de-repressed and could be induced during exponential growth on non-glycolytic compounds metabolized directly into pyruvate, acetyl-CoA or tricarboxylic acid cycle intermediates. 4. The major difference in the metabolism of the organism utilizing these two groups of compound was the existence of high activities of phosphoenolpyruvate carboxylase required for gluconeogenesis. 5. It is concluded that the de-repression of glucosaminidase occurs when the only principal change detected in the intermediary metabolism of the organism was the presence of high activities of phosphoenolpyruvate carboxylase. 6. When the organism was grown on media containing repressing compounds, the enzyme was only de-repressed on entry of the cells into the initial stages of sporulation, where phosphoenolpyruvate carboxylase activity, even in the presence of excess of glucose, increased in parallel with glucosaminidase, neutral proteinase and alkaline phosphatase activities. 7. These results suggest a strong link, at the level of the tricarboxylic acid cycle, between the control of phosphoenolpyruvate carboxylase and the control of the de-repression of glucosaminidase and sporulation.


Association of a ribonuclease with the 50-S ribosomal subunit of Escherichia coli MRE 600.


Protein synthesis by rat cardiac muscle myofibrils.


Physical characterization of alpha-amylase inhibitors from wheat.


Bovine intestinal calcium-binding proteins. Purification and some properties.


The myoglobin of primates. 4. New World monkeys: Cebidae: (1) Saimiri sciureus (squirrel monkey); (2) Lagothrix lagothricha (Humboldt's woolly monkey). Callitrichidae: Callithrix jacchus (common marmoset).


Snake venom toxins the amino acid sequences of two toxins from Ophiophagus hannah (King cobra) venom.


Evaluation of a free thyroxine index.

Evaluation of a free thyroxine index.


Changes in glomerular filtration rate during long-term treatment with propranolol in patients with arterial hypertension.


Clinical applications of thyrotrophin-releasing hormone.


The disposition of propranolol. 3. Decreased half-life and volume of distribution as a result of plasma binding in man, monkey, dog and rat.


Comparable behavior maintained under fixed-ratio and second-order schedules of food presentation, cocaine injection or d-amphetamine injection in the squirrel monkey.


CI-750, A novel antidiarrheal agent.

CI-750, A novel antidiarrheal agent.


Pharmacokinetic study of a patient with diphenylhydantoin toxicity.


The effects of verapamil on metabolism and contractility of the toad skeletal muscle.


Inhibition by L-triiodothyronine and dexamethasone of TSH response to thyrotropin releasing hormone in man.


Transgastric jejunal intubation.

Transgastric jejunal intubation.


Effect of intravenous polyunsaturated phosphatidyl choline in experimental atherosclerosis.


Histochemical distribution of phosphorylase in the central nervous system of primates, Saimiri sciureus and Macaca mulatta.


Surface ultrastructure of reproductive tract in female crab-eating (M. fascicularis) and bonnet (M. radiata) macaques.


Lateral hypothalamic nuclei in the macaque (Macaca mulatta) brain: myeloarchitectonics.


Weight loss following thermal injury.

Weight loss following thermal injury.


Beta-blocking agents in hay fever.

Beta-blocking agents in hay fever.


Penicillins for Haemophilus infections.

Penicillins for Haemophilus infections.


Isolation of alpha-fetoprotein-producing cells from Yoshida ascites sarcoma and its clone.


Presence of antigen related to the carcinoembryonic antigen in feces of normal adults.


Tin-117m: production, chemistry and evaluation as a bone-scanning agent.


Structure and biosynthesis of basement membranes.


Evolution of asymbiotic nitrogen fixation.


The 1974 reorganization of the British National Health Service--aims and issues.


Sternoarticular septic arthritis in heroin users.


Impaired carbohydrate metabolism during a mild viral illness.


Regulation of protein synthesis in rat liver cell-free systems by amino acids.


Photon interaction processes--their relative importance in bone and muscle.


Inhibition of respiration in the isolated eccrine sweat gland by ethacrynic acid.


Dosimetry for total-body electron-beam therapy in the treatment of mycosis fungoides.


Cartilaginous esophageal ring: a cause of esophageal stenosis in infants and children.


Achievement of uniform dose without overlap in multi-port treatment fields, including inter-port shaped blocks.


Chromomeres and genes.

Chromomeres and genes.


Puffing patterns in Drosophila melanogaster and related species.


Replication in polytene chromosomes.

Replication in polytene chromosomes.


A venereological panorama.

A venereological panorama.


The Australia antigen in Brazilian blood donors.


Biosynthesis of vitamin B12 by mesophilic and thermophilic strains of Bacillus megaterium.


Development of tolerance to street heroin in addicted and nonaddicted primates.


Parenteral feeding of surgical patients.


The hydrogen clearance method in assessment of blood flow in cortex, white matter and deep nuclei of baboons.


Neurofibroma of the larynx occurring with Von Recklinghausen disease. Report of a case.


The chain length of the glycans in bacterial cell walls. The glycan chain length of peptidoglycan was measured by reduction with NaB(3)H(4) and isolation of the resulting muramitol, indicative of the length of the chains as biosynthesized, and glucosaminol, which measured the length of the chains after rupture by endo-beta-N-acetylglucosaminidases. Measurement of the non-reducing terminal N-acetylglucosamine by Smith degradation confirmed the result.


Influence of dexamethasone on TRF induced TSH release in rats.


A renewal attempt to compare the affinity of thyroxine-binding-globulin to thyroxine in normal and hyperthyroid sera.


Spontaneous firing patterns of epileptic neurons in the monkey motor cortex.


Behavioral impairment in the monkey following implantation of aluminum hydroxide on the temporal cortex: the role of cortical destruction.


Responses of single neurons in the auditory cortex of squirrel monkeys to variants of a single call type.


Serological studies of Haemophilus influenzae. 3. The endotoxic effect of various antigen preparations and the relation between this effect and demonstrable precipitinogens.


Studies in immunodermatology. 3. Induction of intraepithelial lesions in monkeys by intramucosal injections of pemphigus antibodies.


Cromolyn in extrinsic and intrinsic asthma.


Increased sensitivity to egg albumin in rats fed pristane.


Enzymatic cleavage of (+)citramalate into pyruvate and acetyl-CoA in Bacillus sp.


Interaction of chicken erythrocyte AMP deaminase with chelating agents.


Spectroscopic properties of C-phycocyanin and of its alpha and beta subunits.


Reactivity of oxytyrosinase toward substrates.


Primary structure of human fibrinogen and fibrin. II. Structural studies on NH2-terminal part of gamma chain.


Purification and properties of an extracellular polysaccharide containing amino sugars formed by Bacillus cereus.


The application of cellulose carbonate to the preparation of water-insoluble immunoadsorbents used in the purification of antibodies to immunoglobulins.


Further studies on the reaction of partial identity in quantitative immunoelectrophoretic patterns.


Circularity of the Bacillus subtilis chromosome and further studies on its bidirectional replication.


Biochemical properties of a cephalosporin beta-lactamase from Pseudomonas aeruginosa.


Endotoxemia in febrile reactions during hemodialysis.


Preparation and characterization of mouse intestinal phospholipase.


Lowering of serum cholesterol by intestinal bacteria in cholesterol-fed piglets.


Societal and individual determinants of medical care utilization in the United States.


Separation of small circular DNA molecules from the blue-green alga Anacystis nidulans.


X-ray diffraction of muscle labelled with antibody to troponin-C.


Miniature eye movement.

Miniature eye movement.


Slowed decay of the monkey's cone receptor potential by intense stimuli, and protection from this effect by light adaptation.


Human leukemia, nucleic acids and viruses. Hypotheses and perspectives.


Some characteristics of the proliferative activity of erythroblasts in untreated and treated acute leukaemia.


Transplantable myeloid rat leukaemia induced by 7,12-dimethylbenz(a)anthracene.


Acute myelomonocytic leukemia in a patient with Hodgkin's disease.


Monoclonal origin of acute transformation of chronic myelogenous leukemia. Evidence from an acquired sex chromosome mosaic.


Fetal factor VIII and IX levels in early pregnancy and their significance in prenatal diagnosis.


Accumulation of drugs on melanin.

Accumulation of drugs on melanin.


Angiographic abnormalities in the urinary bladder wall after irradiation. I. Animal experiments. II. Clinical investigation.


Turner syndrome with partial deletion of the X chromosome long arm.


Visual sensitivity.

Visual sensitivity.


Perception.

Perception.


Basic drives.

Basic drives.


Role of inorganic nitrite in methaemoglobin formation after nitrate ester administration to the rat. 1. Methaemoglobin is rapidly formed during the incubation of rat red cells with inorganic nitrite, whereas ethylene glycol dinitrate at a higher concentration has little effect.2. The concentrations of blood inorganic nitrite in the rat after ethylene glycol dinitrate or glyceryl trinitrate injection can be reproduced by the infusion of nitrite. The methaemoglobin formed after ethylene glycol dinitrate or glyceryl trinitrate injection is equivalent to that produced from nitrite infusions simulating its formation from the nitrate esters.3. It is concluded that methaemoglobin arising from nitrate ester administration is formed principally by the action of the metabolite inorganic nitrite.


The action of Bacillus subtilis liquefying amylase on 6-deoxy-6-iodoamylose.


The effects of intravenous and intraduodenal feeding on nitrogen balance after surgery.


A genetic scheme for the blood systems ABO, Rh and MNSs, and an explanation of antigen-antibody patterns.


Iron metabolism of premature infants and their mothers.


The effect on reaction to painful stimuli of lesions in the centromedian nucleus in the thalamus of the monkey.


The surgical production of chronic uremia in primates.


Alpha-D-fucosidase from Aspergillus oryzae: characterization of alpha-D-fucosidase with alpha-D-galactosidase activity.


Na+-dependent transport of threonine in Brevibacterium flavum.


A mathematical model for the determination of viscoelastic behavior of brain in vivo. II. Relaxation response.


Studies of Ternidens deminutus Railliet & Henry, 1909 (Nematoda). I. External morphology.


High voltage electron microscopy of cellular changes in the wet state.


Resynchronization of RNA synthesis by coliphage Qbeta replicase at an internal site of the RNA template.


A common conformational feature in several prokaryotic and eukaryotic 5 S RNAs: a highly exposed, single-stranded loop around position 40.


The quantitative histochemistry of acid proteinase in the nervous system: localization in neurons.


Cost-benefit analysis of in vitro screening tests of thyroid function.


Retreatment of recurrent nasopharyngeal malignancy using a radium mold.


Prevention of infection of pacemaker pocket.


Experimental comparison of radioactive agents for studies of the placenta.


Urinary antidiuretic hormone excretion during the menstrual cycle and pregnancy in the monkey.


The nurse and the high-risk infant.

The nurse and the high-risk infant.


Total intravenous nutrition by peripheral vein in neonatal surgical patients.


Kinetic analysis of the base sequence heterogeneity of RNA molecules by RNA-DNA hybridization.


Studies of the sudden infant death syndrome in King County, Washington. IV. Immunologic studies.


Role of the skin in amphibian sodium metabolism.


Hybridization of nucleic acids to chromosomes.


Some aspects of respiratory function in three siblings with Morquio-Brailsford disease.


Qualitative and quantitative comparison of C3 variant sera during the Bonn workshop.


Haemolytic disease of the newborn caused by the rare Rhesus antibody anti-CX.


Immunological dosage of Hb F and its correlation with the Singer test. Decrease of fetal hemoglobin with age.


Report of a proficiency test for the hepatitis-B antigen.


An improved automated method for the estimation of sialic acid released in the neuraminidase assay.


Substrate specificity of a human-specific esterase.


Immunodiffusion reaction with animal influenza viruses and antibody.


The disorder of hyaluronic acid metabolism in cultured skin fibroblasts derived from a patient with the Hurler syndrome. The metabolism of hyaluronic acid in cultured skin fibroblasts derived from a patient with the Hurler syndrome and from a normal subject was examined. 1. An increased net incorporation of [(3)H]glucose into the hyaluronic acid fraction of the Hurler-syndrome cells occurred when compared with normal cells. 2. During a ;chase' period, approx. 35% of the radioactivity derived from glucose was lost from the hyaluronic acid fraction of the Hurler-syndrome cells, whereas the normal cells retained all their radioactivity. 3. Although the Hurler-syndrome cells contained a ninefold greater amount of hyaluronic acid than normal cells, simultaneous determination of the specific radioactivity derived from the label revealed a value for the Hurler-syndrome cells one-half that of normal cells. These results are taken to indicate that the Hurler cells synthesize hyaluronic acid de novo at a higher rate than do normal cells. 4. Exposure of Hurler-syndrome cultured fibroblasts to a crude urine corrective-factor preparation (Neufeld & Cantz, 1971), now known to contain alpha-l-iduronidase, the specific Hurler-syndrome corrective factor (Bach et al., 1972), decreased the hyaluronic acid content to near-normal values before any effect was observed on [(3)H]glucose incorporation into the hyaluronic acid fraction. 5. In addition, the hyaluronic acid content of the normal cells decreased after exposure to the corrective factor of urine. 6. The mobilization of hyaluronic acid in Hurler-syndrome and normal cells exposed to the crude corrective-factor preparation of urine caused a decrease in specific radioactivity in the ;corrected' Hurler-syndrome cells and an increase in specific radioactivity in the ;corrected' normal cells.


Isolation and characterization of membrane-bound polysomes from ascites tumor cells.


ATP regeneration using immobilized carbamyl phosphokinase.


Immunochemical characterization of commercial protease products.


Differences in the urea-extracted proteins of mouse epidermis and squamous cell carcinomata determined by fluorescence microscopy. Fluorescence microscopy was used to demonstrate differences in the urea-extractable antigens of mouse epidermis and squamous cell carcinoma. When serum- and normal tissue sediment-absorbed antisera prepared against mouse epidermal urea-extracted proteins were further absorbed with carcinoma urea antigens, antisera specific for epidermis resulted. When antisera raised against the urea-extractable proteins of mouse squamous cell carcinomata were serum- and normal tissue sediment-absorbed and then further absorbed with epidermal urea antigens, antisera were prepared which stained papilloma and carcinoma, but not epidermis, and thus these antisera were not specific for carcinoma. Antisera prepared against the urea-extractable proteins of human epidermis reacted in immunodiffusion in agar with the epidermal urea proteins, but not with human squamous cell carcinoma urea proteins. Also antisera prepared against the carcinoma urea-extractable proteins reacted with these proteins in agar, but no reaction occurred with the epidermal urea-extractable proteins.


Mechanisms of selective uptake of 2-methyl-4-naphthoquinol bis disodium phosphate, into some mammalian cells in tissue culture. Earlier papers have described the selective uptake of the radioactive drug 2-methyl 6,7-ditritio-1, 4-naphthoquinol bis disodium phosphate into some human tumour cells in tissue culture. This work examines these results in greater detail. The kinetics of the uptake process into HEp/2 cells and its precise dependence on the experimental conditions are described. It is shown that an alkaline phosphatase (APase) is present on the surface of HEp/2 cells and that this is responsible for the uptake of label. L cells do not take up the radioactive label and are shown not to possess significant quantities of APase.


Metabolic effects of PCB (polychlorinated biphenyls) on the brown trout (Salmo trutta).


Free radical mechanism for phenylhydrazine action on the retractor unguis muscle of the grasshopper, Romalea microptera.


Vorticella--a model for chemopharmacodynamic action on smooth muscle.


The relationship between total body potassium and exchangeable body potassium measured at 24 hours and 44 hours after administration of 43K.


Sympathetic innervation of the ciliary muscle in monkeys.


Pattern of recruitment of oocytes to second growth phase in normal toads, and in hypophysectomized toads, Bufo bufo bufo (L.), treated with gonadotropin (HCG).


The effect of lipids from Listeria monocytogenes on immune response in mice.


The immunoglobulins derived from a VH-CH recombinant rabbit and its normal relatives. I. Measurements of heavy chain molecular weights and allotypes of colostral IgA.


Plasmocid encephalopathy in the rhesus monkey: a study of selective vulnerability.


Prostaglandin E2 and gastric acid secretion in man. 1. Extracts of human stomach homogenized in Krebs solution had more PGE(2)-like activity than tissue homogenized in acid/ethanol or in the presence of indomethacin, indicating that the tissue can synthesize PGE(2).2. The distribution and synthesis of PGE(2)-like substance in human stomach was determined by extracting frozen sections cut parallel to the mucosal surface. Peak levels usually occurred at a depth of 0-600 mum in the mucosa.3. Small amounts of a PGE(2)-like substance were present in basal gastric juice, and its concentration was usually even lower in secretion stimulated by pentagastrin or histamine.4. Submaximal acid secretion produced by I.V. infusion of pentagastrin generally fell slightly when indomethacin was administered rectally to inhibit PG synthesis.5. These experiments, together with the findings that orally administered PGE compounds do not inhibit human gastric acid secretion, seem to argue against a possible inhibitory role for PGE(2) in gastric acid secretion in man.6. If this is so, it would follow that gastric bleeding caused by aspirin-like drugs is not due to increased acid secretion. A hypothesis is presented that tissue damage following vasoconstriction and ischaemia, due to inhibition of PG synthesis in blood vessels, contributes to the bleeding.


The dangers of oral feeding in the presence of cuffed tracheostomy tubes.


Ultrastructure and functional states of mitochondria. Effect of fixatives on the stabilization of unstable configuration.


Reversibly temperature sensitive phototransduction mutant of Drosophila melanogaster.


Neurofibromatosis (Von Recklinghausen's disease) and juxta-medullary spinal lipoma.


Cortical bone remodeling and bone mass in primary osteoarthrosis of the hip.


Contributions to the optimal use of human blood. II. The large-scale preparation of prothrombin complex. A comparison between two methods using the anion exchangers DEAE-cellulose DE 52 and DEAE-sephadex A-50.


The presence of I, H, HI, and i blood group substances in the tissues of rabbits, monkeys, and guinea pigs.


Glycogen synthesis in rat diaphragm in vivo: a biphasic effect of insulin on glycogen synthetase enzyme.


Adenohypophysial growth and thyroxine binding: effects of steroid hormones.


Agenesis of the corpus callosum in the median facial cleft syndrome and associated ocular malformations.


The epidemiology of hepatitis B infections in Africa: results of a pilot survey in the Republic of Senegal.


Ocular abnormalities of a child associated with familial microcephaly.


Formaldehyde-glutaraldehyde fixation.

Formaldehyde-glutaraldehyde fixation.


Tissue response to the movement of bones.


Faciolingual tooth movement: its influence on the root and cortical plate.


The uptake of 2-deoxy-D-glucose by Pseudomonas aeruginosa and its regulation. The non-metabolizable glucose analogue 2-deoxy-d-glucose is taken up by Pseudomonas aeruginosa against a concentration gradient, in a predominantly unchanged form. d-Glucose competitively inhibits 2-deoxy-d-glucose uptake and also causes a rapid exit of intracellular 2-deoxy-d-glucose. Thus these two sugars share the same stereospecific carrier system, and glucose transport can be studied reliably with 2-deoxy-d-glucose. The transport system is inducible, and is strongly repressed by a number of organic acids such as acetate, citrate, succinate, fumarate and malate, even in the presence of adequate excess of the inducer (d-glucose). Repression by organic acids can be relieved by transferring cells to a glucose medium, but in the presence of chloramphenicol the cells fail to recover from repression, indicating that the formation of the transport system involves the synthesis of protein. The results demonstrate that the regulation of glucose metabolism effected by citric acid-cycle intermediates in P. aeruginosa is manifest at the level of the glucose-transport system.


Chemostat studies on the regulation of glucose metabolism in Pseudomonas aeruginosa by citrate. The effect of the relative concentrations of citrate and glucose on the regulation of key enzymes of the direct oxidative, phosphorylative, Entner-Doudoroff and pentose-cycle pathways of glucose metabolism in Pseudomonas aeruginosa has been investigated in continuous culture under conditions of NH(4) (+)-limitation. For comparison isocitrate dehydrogenase and aconitase were also assayed. Measurements were made for steady-state and transient conditions and the effect of growth rate was also studied. When cells grew on 75mm-citrate the glucose concentration had to attain 6-8mm before significant induction of enzymes of glucose metabolism occurred; the specific activities increased further as the result of both raising the glucose concentration to 30mm and then subsequently lowering the citrate to 60mm and then to 45mm. The specific activities of the glucose enzymes increased immediately during the transient period between the steady states characteristic of growth on 6mm- and 8mm-glucose, the increase continuing for about two doubling times. The converse experiment of adding increasing citrate concentrations to 45mm-glucose medium revealed an immediate induction of the citrate-transport system, oxidation of citrate following the increase in citrate concentration up to 8mm. Between 8mm- and 16mm-citrate a marked repression of gluconate, glucose 6-phosphate and 6-phosphogluconate dehydrogenases and the Entner-Doudoroff enzymes occurred. Increased growth rate in citrate medium resulted in decreased specific activities of glucose 6-phosphate dehydrogenase and isocitrate dehydrogenase. Increased growth rate in citrate-glucose medium gave decreased specific activities of isocitrate dehydrogenase and aconitase whereas the activities of some of the glucose enzymes decreased initially but then increased at the highest growth rate (0.5h(-1)), at which a marked increase in glucose utilization occurred. These observations accord with the regulation of glucose enzymes by induction with glucose or its metabolites and repression by citrate or its metabolic products.


The regulation of transport of glucose and methyl alpha-glucoside in Pseudomonas aeruginosa. The methyl alpha-glucoside-transport system of Pseudomonas aeruginosa has been characterized with respect to its specificity, energy-dependence, kinetics and regulation. The uptake of glucose involved two components, one of which transported glucose (K(m)=8mum) and methyl alpha-glucoside (K(m)=2.8mm) whereas the other was more complex, involving the extracellular activity of glucose dehydrogenase. Mutants defective in this enzyme have been isolated and characterized. The methyl alpha-glucoside-glucose-transport system was repressed when the organism was grown in the absence of glucose; the induction of this transport system by glucose, and its activity once induced, were inhibited by products of citrate metabolism.


Interrelationship of islet metabolism, adenosine triphosphate content and insulin release. The oxidation of some exogenous substrates and their effects on ATP content and insulin release in mouse pancreatic islets were measured. The ATP concentration of islets incubated without exogenous substrate shows a gradual decrease, which can be prevented by glucose or mannose (20mm) or leucine (2.5mm); d-glyceraldehyde (5mm) is as effective as glucose (5mm); fructose or N-acetylglucosamine (20mm), pyruvate (10mm) and dl-3-hydroxybutyrate (2mm) are less effective; galactose (20mm), acetate (10mm), octanoate (2mm) and succinate (10mm) have no ATP-maintaining ability. Islets oxidize glucose, mannose, glyceraldehyde, leucine and, less readily, N-acetylglucosamine and glucosamine; galactose, however, is poorly metabolized. Mannoheptulose inhibits the oxidation of glucose but not of glyceraldehyde. Insulin release, measured over a 2h incubation, is stimulated by glucose, mannose, leucine, glyceraldehyde or glucosamine but not by fructose or N-acetylglucosamine. The latter, however, potentiates the effects of glucose or glyceraldehyde (5mm) or leucine (2.5mm) on release; the potentiating effects are inhibited by mannoheptulose, which also blocks glucose-, but not glyceraldehyde- or leucine-stimulated release. In the presence of glucose (20mm), metabolic inhibitors depress insulin release and islet ATP content in parallel. However, rates of insulin release and ATP content measured after incubation with various combinations of exogenous substrates do not appear to be correlated. Sulphonylureas stimulate insulin release but decrease islet ATP concentrations. These results provide further evidence of a close association between the metabolic activity of exogenous substrates and their ability to initiate insulin release. Glucoreceptor models are formulated in the light of these observations and discussed.


Toxicologic and pharmacologic aspects of rifampin.


Influence of insulin and glybenclamid on the intestinal disaccharidases of subtotally pancreatectomized rats.


Effect of protein-calorie malnutrition on the development of social behavior in rats.


Mandibular bone grafting in trauma.

Mandibular bone grafting in trauma.


Injuries of the face and jaws in children.


A technique of vestibular plasty using a free mucosal graft from the cheek.


Reversibility of surgically induced dental ankylosis in rats.


Serum IgD concentrations in children with atopic diseases.


Neutral sucrose sedimentation of very large DNA from Bacillus subtilis. I. Effect of random double-strand breaks and centrifuge speed on sedimentation.


Neutral sucrose gradient sedimentation of very large DNA from Bacillus subtilis. II. Double-strand breaks formed by gamma ray irradiation of the cells.


Relation between single-strand DNA mass and sedimentation distance in alkaline sucrose gradients.


Intravenously induced infantile intoxication with ethanol.


Hypothalamic gigantism.

Hypothalamic gigantism.


Pigmented lesions of incontinentia pigmenti in a neonate.


Synthesis of (plus-)-trans-11-methylthio-1,2,3,4,6,7,12,12b-octahydrohydroxyindolo(2,3-a)quinolizine.


Antigenicity of vascular heterografts.

Antigenicity of vascular heterografts.


Studies on the primitive lymphatic apparatus in the blood vascular system of the lungs of Macaca cyclopis.


The M. temporalis of the Formosan monkey.


The failing blade-vent implant.

The failing blade-vent implant.


Isolation and partial characterization of arachin-P6.


Isolation of the teichoic acid of Bacillus subtilis 168 by affinity chromatography.


Toxicity of systemic insecticides. Toxicological considerations in using of ganophosphorus insecticides.


Lean diets and atherosclerosis.

Lean diets and atherosclerosis.


Effects of immunizing turkeys with ovine luteinizing hormone.


Treatment and prevention of childhood lead poisoning: new approach.


Bioavailability studies of nitrates in the dog.


The metabolism of 5,5-diphenylhydantoin (DPH) in nonpregnant and pregnant Rhesus monkeys.


Studies on acute phase proteins of rat serum. 3. Site of synthesis of albumin and alpha 1-acid glycoprotein and the contents of these proteins in liver microsome fractions from rats suffering from induced inflammation.


The amino acid sequence of Streptomyces griseus trypsin. I. The peptic peptides.


Variations in genetic architecture at different doses of gamma-radiation as measured by longevity in Drosophila melanogaster.


The principal liver carcinogen-protein conjugate after a single dose of hepatic azocarcinogen.


Immunochemical studies on lactate dehydrogenase: preparation and characterization of anti-M lactate dehydrogenase antiserum: development of an immunochemical assay for lactate dehydrogenase isoenzymes in human serum.


Quantitation of human IgG by rocket immunoelectrophoresis at pH 5 by use of carbamylated antibodies. A routine laboratory method.


Serum immunoglobulins, complement component levels and autoantibodies in liver disease.


Immunoglobulin alterations associated with heroin addiction.


Immunological studies in children undergoing tonsillectomy.


Anti-chromium barrier creams.

Anti-chromium barrier creams.


Factors affecting care of the patient: clients, government policy, role of the family and social attitudes.


Effect of aging on the marmoset periodontium.


Some factors affecting precipitation and complex formation of an IgG cryoglobulin.


In vitro synthesis of immunoglobulins, secretory component and complement in normal and pathological skin and the adjacent mucous membranes.


Verrucous carcinoma of the mouth.

Verrucous carcinoma of the mouth.


Familial gingival fibromatosis: an unusual pathology.


Mitostatic action of vinblastine sulphate on oral epithelia of hamsters.


Changes in cell cycle characteristics in hamster cheek pouch epithelium during treatment with DMBA.


Scanning electron microscopy of calcium hydroxide induced bridges.


Fibrous dysplasia: a nosologic problem in the diagnosis of fibro-osseous lesions of the jaws.


Isolation and properties of Bacillus subtilis strains lysogenized by a clear plaque mutant of bacteriophage phi 105. A clear plaque mutant of the temperate Bacillus phage phi105 lysogenized a small fraction of infected cells forming an integrated prophage at or near the normal phi105 insertion site. These lysogens exhibited a spontaneous induction rate approximately 1,000-fold lower than wild type and were noninducible (ind(-)) by mitomycin C. Prophage was induced, however, when competent cultures were incubated with transforming DNA. The ind(-) phenotype could not be attributed solely to the clear plaque mutation and appears to involve a cell-specific factor. Lysogenization by the clear plaque mutant, in contrast to wild-type phage, did not cause a marked reduction in transformation efficiency.


DNA-mediated prophage induction in Bacillus subtilis lysogenic for phi 105c4. Prophage was induced when strains of Bacillus subtilis 168 lysogenic for phi105c4 were grown to competence and exposed to specific bacterial DNAs. The time course of phage production was similar to that observed for mitomycin C induction of wild-type prophage. Induction was directly dependent upon DNA concentration up to levels which were saturating for the transformation of bacterial auxotrophic markers. The extent of induction varied with the source of DNA. The burst of phage induced by DNA isolated from a W23 strain of B. subtilis was fivefold less than that induced by DNA from B. subtilis 168 strains, while B. licheniformis DNA was completely inactive. This order of inducing activity was correlated with the ability of the respective DNAs to transform auxotrophic markers carried by one of the phi105c4 lysogens. Differences in inducing activity also were observed for different forms of phi105 DNA. The DNAs isolated from phi105 phage particles and phi105c4 lysogens were inactive, whereas DNA from cells lysogenized by wild-type phi105 induced a burst of phage. When tested for transforming activity, however, both phi105c4 and phi105 lysogen DNAs were equally effective. An induction mechanism which involves recombination at the prophage insertion site is proposed to explain these differences.


Synthesis of bacteriophage phi 29 proteins in Bacillus subtilis. Seventeen bacteriophage phi29 proteins were detected in ultraviolet light-irradiated Bacillus subtilis by autoradiography of polyacrylamide slab gels. The appearance of phi29 proteins occurred either before or concomitantly with viral DNA replication. Viral proteins detected early in the infectious cycle consisted of nine polypeptides ranging from 5,200 daltons to 54,000 daltons. Two of the early proteins were identified as, respectively, the major capsid protein and the protein comprising the filaments which extend from the head of the virus. Late phi29 proteins were composed of eight polypeptides ranging from 14,000 daltons to 95,000 daltons. Only three late proteins were noncapsid proteins. Among the early proteins, six were synthesized at diminishing rates late in the infectious cycle. One of the early proteins (protein 12) lacked histidine, whereas two (proteins 10 and 15) lacked tryptophan. Among the 17 proteins detected, 10 were viral noncapsid proteins. The amount of viral genetic information required to code for the 17 proteins detected in these experiments (81% of the potential genetic information of phi29 DNA) compares favorably with the genetic information detected as mRNA in a previous report, 85% of the potential information on the phi29 chromosome.


Effects of actinomycin D and ultraviolet and ionizing radiation on Pichinde virus. Actinomycin D (0.05 mug/ml) suppresses the synthesis of ribosomal RNA of baby hamster kidney (BHK21) cells. The production of infectious Pichinde virus was enhanced in the presence of actinomycin D, although the production of virus particles was not substantially different from cultures inoculated in the absence of the drug. By prelabeling BHK21 cells with (3)H-uridine and then allowing the virus to replicate in the presence of actinomycin D, it was possible to show that ribosomal RNA synthesized prior to infection was incorporated into the virion. A single-hit kinetics of inactivation of Pichinde virus was observed with ultraviolet light, suggesting that the virus contains only a single copy of genome per virion. Comparison of the inactivation kinetics by gamma irradiation of Pichinde virus with Sindbis and rubella virus indicated that the radiosensitive genome of Pichinde virus was about 6 x 10(6) to 8 x 10(6) daltons. This value is greater than the 3.2 x 10(6) daltons which was estimated by biochemical analysis. One possible explanation considered is that the ribosomal RNA of host cell origin is functional and accounts for the differences in genome size estimated by the two methods.


Heat induction of prophage phi 105 in Bacillus subtilis: bacteriophage-induced bidirectional replication of the bacterial chromosome. A mutant of Bacillus subtilis, dna-1, which cannot initiate new rounds of DNA replication (obtained from N. Sueoka) was lysogenized with wild-type phi 105 and with the heat-inducible mutant phi 105 cts23. Bacteria were incubated at the permissive temperature in the presence of chloramphenicol and then shifted to the nonpermissive temperature where induction of phi 105 cts23 occurs. DNA made after the shift was labeled with a density label, and the distribution of bacterial and phage markers in replicated and unreplicated DNA was determined. Similar experiments were performed with nonlysogenic dna-1 infected with phage phi 105 cts23 after the temperature shift. The results show that after induction of phi 105 cts23 prophage, bacterial markers on either side of the prophage replicate at an increased rate compared to more distant markers. No selective stimulation of bacterial DNA synthesis was observed on infection or after shifting bacteria lysogenic for noninducible phage to the higher temperature. Attempts to suppress the initiation mutation dna-1 by phage phi 105 were unsuccessful.


A survey of mycobacterial isolates from the Randwick Chest Hospital, Sydney, in an 18-month period, 1970 to 1971.


Sodium cromoglycate in the treatment of chronic allergic rhinitis.


The management of the sick diabetic--the physician's role in the prevention of diabetic coma.


Clinical utilization of plasmapheresis and cyclophosphamide in the treatment of malignant lymphoproliferative processes.


The influence of various dietary ascorbic acid levels on serum ascorbic acid and serum cholesterol values of the baboon (Papio ursinus) during captivity.


Effect of ethylenebis (dithiocarbamic acid)disodium salt(nabam)and ethylenebis(dithiocarbamato)manganese(maneb) on Xenopus laevis development.


Appendicular skeletal and visceral malformations induced by thalidomide in bonnet monkeys.


Teratogenic effects of 5-bromodexyuridine on the external morphology of Drosophila melanogaster.


Immunological studies on Naja nigricollis antivenin.


Immunological studies on Egyptian cobra antivenin.


Iodination of cobrotoxin.

Iodination of cobrotoxin.


Changes in antigenic character of fresh Australian snake venom upon aging.


Purification of Aphanizomenon flos-aquae toxin and its chemical and physiological properties.


Immunological studies of scorpion (Buthus minax, L. Koch) venom.


Von Willebrand's syndrome presenting as an acquired bleeding disorder in association with a monoclonal gammopathy.


Chronic hemolytic anemia due to cold agglutinins: a 20-year history of benign gammopathy with response to chlorambucil.


Menstrual cycle characteristics and side of ovulation in the Rhesus monkey.


A comparative study of the mechanisms of passive cutaneous anaphylaxis induced by mouse IgG, rabbit IgG, and rabbit F (ab')2 antibodies. I. The role of serotonin and histamine.


The cardiac auto-immune system. IX. Auto-immune myocarditis in rabbits immunized with rabbit heart.


The canine respiratory immune response.

The canine respiratory immune response.


Suppression of anaphylactic responses in mice by antilymphocyte sera, normal sera, and egg albumin.


Reduction by serum proteinase inhibitors of B. subtilis proteinase-induced hypersensitivity reactions.


Human leukocyte migration inhibition as an indicator of cellular hypersensitivity to soluble antigens.


Effects of phagocytosis by rabbit granulocytes on macromolecular synthesis and degradation in different species of bacteria. Phagocytosis and killing of gram-positive Bacillus megaterium and Micrococcus lysodeikticus by granulocytes in vitro is associated with almost immediate cessation of bacterial protein synthesis. By contrast, protein synthesis by Escherichia coli continues after ingestion and killing. After preincubation of E. coli with intact granulocytes for 15 min, when 95% or more of the bacteria can no longer multiply, induction of beta-galactosidase proceeds at rates about half of control values. With disrupted granulocytes, which kill E. coli as rapidly as intact cells, the rate of induction of beta-galactosidase does not fall until after 30 min of preincubation. We attribute the different effects of phagocytosis on the biochemical apparatus of these microorganisms to the different fates of their envelopes. Specifically labeled protein, ribonucleic acid, deoxyribonucleic acid, and lipid of all three species of bacteria and peptidoglycan of E. coli are apparently incompletely degraded during phagocytosis. However, the cell walls of M. lysodeikticus and B. megaterium undergo rapid and almost complete degradation. The resulting structural disintegration of these gram-positive microorganisms must cause extensive biochemical disorganization as well. Our evidence indicates that the E. coli envelope, on the other hand, retains sufficient structural organization to preserve integrated biochemical function for at least 1 h after the bacteria have lost the ability to multiply.


Guanosine diphosphate-L-fucose glycopeptide fucosyltransferase activity in Corynebacterium insidiosum. The biosynthesis of a phytotoxic glycopeptide of Corynebacterium insidiosum involves guanosine diphosphate-l-fucosyltransferase activity. This enzyme activity is most consistently associated with the cellular membranes fraction. The optimal pH for the transfer reaction is 7.5. The partially hydrolyzed toxin serves as an acceptor (primer) of l-fucose.


Specificity of the tyrosine-phenylalanine transport system in Bacillus subtilis. l-Tyrosine and l-phenylalanine enter cells of Bacillus subtilis via a system of active transport that exhibits complex kinetic behavior. The specificity of the transport system was characterized both at low concentrations of transport substrate (where affinity for l-tyrosine or l-phenylalanine is high but capacity is low) and at high concentrations (where affinity is low but capacity is high). Specificity was not found to differ significantly as a function of either l-tyrosine or l-phenylalanine concentration. Kinetic analysis showed that the relationship between the uptake of l-phenylalanine and l-tyrosine is strictly competitive. Neither l-tyrosine nor l-phenylalanine uptake was competitively inhibited by other naturally occurring l-amino acids, indicating the importance of the phenyl side chain to uptake specificity. Hence, it is concluded that l-tyrosine and l-phenylalanine are transported by a common system that is specific for these two amino acids. The abilities of analogue derivatives of l-tyrosine and l-phenylalanine to inhibit the uptake of l-[(14)C]tyrosine and l-[(14)C]phenylalanine competitively were determined throughout a wide range of substrate and inhibitor concentrations. In this manner, the contributions of the side chain, the alpha-amino group and the carboxyl group to uptake specificity were established. It is concluded that the positively charged alpha-amino group contributes more significantly to uptake specificity than does the negatively charged carboxyl group. The recognition of a phenyl ring is an essential feature of specificity; other amino acids with aromatic side chains, such as the indole and imidazole rings of l-tryptophan and l-histidine, do not compete with l-tyrosine and l-phenylalanine for uptake. The presence of the p-hydroxy substitutent in the side chain (as in l-tyrosine) enhances the uptake of the aryl amino acid analogues investigated.


Ultrastructural studies of sporulation in a conditionally temperature-sensitive ribonucleic acid polymerase mutant of Bacillus subtilis. Morphological studies of a conditionally temperature-sensitive ribonucleic acid polymerase mutant of Bacillus subtilis have revealed that sporulation is inhibited at stage II when the cells are grown at 47.5 C. Growth and sporulation occur normally at 30 C with the mutant. The mutant grows normally at 47.5 C but is prevented from sporulating at the nonpermissive temperature by an abnormal septation during forespore membrane formation which prevents the subsequent engulfment process (stage III). The mutation affects the normal functioning of ribonucleic acid polymerase at the nonpermissive temperature resulting in abortive sporulation.


Soluble antigens associated with infection with apple chlorotic leaf spot virus.


Dose distribution for cobalt 60 tangential irradiation of the breast and chest wall.


Treatment of pain in chronic pancreatitis by irradiation.


Cromolyn sodium in the management of the child with asthma: a symposium. Preliminary data from a collaborative group study.


Reflections and thoughts on the use of cromolyn sodium in the asthmatic child.


Experiences with cromolyn sodium in asthma in children.


Personal observations on cromolyn sodium prophylaxis.


Review and assessment of the efficacy of cromolyn sodium, particularly after long-term administration.


Pros and cons of cromolyn sodium prophylaxis. A summary of experiences with 70 asthmatic children.


The new field of plastics toxicology--methods and results.


When a drug is the culprit.

When a drug is the culprit.


Production of macrophage migration inhibition factors by virus-infected cell cultures. Macrophage migration inhibitory (MIF-like) activity was demonstrated in the supernatant fluids from cultures of African green monkey kidney cells (BGM) infected with mumps virus or Newcastle disease virus. We could detect no such activity in noninfected cultures. The virus-induced activity reported here is not due to nonspecific cytotoxic material released by dead or dying cells, and it does not require cell replication for its production. Preliminary estimates of molecular weight by Sephadex G-100 chromatography revealed a broad band of activity associated with the 45,000 and 65,000 markers. These are significantly smaller than previously reported chemotactic substances from virus-infected cultures, and thus appear to represent different cell products. These MIF-like factors may be produced concomitantly with interferon. However, ultraviolet irradiation of appropriate duration abolishes the ability of viruses to induce substances with MIF-like activity while preserving the ability to induce interferon. This strongly suggests that interferon is not the agent responsible for the macrophage migration inhibition effect. The functional properties of these various cell products induced by virus infection suggest that they all may play a role in the response to virus infection in vivo.


Immune response to Mycobacterium tuberculosis in rats. After intravenous injection into rats, both the attenuated strain R1Rv and the virulent strain H37Rv of Mycobacterium tuberculosis grow in the liver and spleen. However, the infected rats mount a specific immune response with great rapidity, giving a false impression of natural resistance to the tubercle bacillus. Adoptive immunity to tuberculosis was achieved by transferring thoracic duct cells from immunized donors to normal syngeneic recipients. The transferred immunity was vested in a population of lymphocytes uncontaminated with macrophages. The adoptive immunity was effectively expressed against both attenuated and virulent tubercle bacilli, and it was shown to be immunologically specific. Lymphocytes which conferred immunity to tuberculosis were not protective against Listeria monocytogenes infection, and vice versa. Immunity could not be transferred with either normal thoracic duct lymphocytes (TDL), heat-killed sensitized TDL, or serum from specifically immunized donors. The ability of TDL from BCG-immunized donors to confer immunity was maintained at an unimpaired level for at least 3 months after immunization.


Extraction, purification, and chemical and immunological properties of the Streptococcus mutans group "a" polysaccharide cell wall antigen. An antigen of Streptococcus mutans has been extracted from HS6 (group "a") whole cells and repeatedly fractionated by Sephadex chromatography. The antigen is shown to be a polysaccharide and contains the S. mutans group "a" antigenic site and also a second antigenic site which is common to "a" strains and 2 of 3 group "d" strains. Immunological electrophoretic and chromatographic data indicate that the two sites exist in a single molecule. The polysaccharide has a molecular weight of 107,000 and is composed of glucose, galactose, glucosamine, and galactosamine. No significant quantities of lipid, phosphorus, glycerol, or ribitol are present. Immunological specificity of the group "a" polysaccharide site depends primarily on a d-glucose . d-glucose sequence, the "a-d" site on a terminal d-galactose. Water at 100 C and pepsin (pH 2.5) at room temperature are very effective in extracting the polysaccharide from lyophilized S. mutans cells. Trypsin and lysozyme are less effective. The antigen-antibody combining site appears to be located at the cell wall surface. A small quantity of enzyme-resistant protein (5%) is firmly linked to the antigen and is considered to be a remnant of a protein to which the polysaccharide is attached in the cell wall. The composition of the protein does not identify it as a part of the peptidoglycan. No reaction to the purified polysaccharide is obtained with antisera specific for teichoic acid glycerophosphate polymers from streptococci, staphylococci, or lactobacilli.


Acute cavitary histoplasmosis in Rhesus monkeys: influence of immunological status. Male rhesus monkeys were randomized into groups according to body weight, immunized with different Histoplasma capsulatum antigens, and two weeks later were infected intratracheally with 10(8)H. capsulatum yeast cells. Complement fixation antibody titers, skin tests, and chest X rays were performed at weekly intervals from immunization until autopsy, at which time the spleens were cultured and the lungs and other organs were dissected. Pulmonary cavities were found in 33% of the animals, and extrapulmonary dissemination was present in 85% of the animals. Delayed hypersensitivity and circulating antibody activity was detected in all animals at some time during the experimental period; however, animals which developed pulmonary cavities had a longer period before circulating antibodies were detected than animals which did not develop pulmonary cavities. Delayed hypersensitivity developed at approximately the same time in both cavitary and noncavitary animals. Early appearance of delayed hypersensitivity was associated with reduced amounts of extrapulmonary dissemination, in that animals with a later onset of skin test reactivity had more H. capsulatum cultured from the spleen. There was no correlation between the onset or titers of circulating antibodies and the spleen culture results.


Immunofluorescent study of the replication of infectious pancreatic necrosis virus in trout and Atlantic salmon cell cultures. Cell cultures of trout gonad tissue (RTG-2) and Atlantic salmon heart, kidney, liver, and spleen tissue were inoculated with 50 50% tissue culture infective doses (TCID(50)) of infectious pancreatic necrosis (IPN) virus per cell, and the titer of cell-associated and released virus was determined from 2 to 16 h postinoculation (PI). Cover slips were collected over the same period and stained for IPN viral antigen by the direct immunofluorescent (FA) technique. Viral replication was detected after a latent period of approximately 2 to 4 h and reached a peak titer of 10(8.2) to 10(8.4) TCID(50) per ml at 8 to 10 h PI. The release of virus was more rapid in Atlantic salmon cells than in RTG-2 cells. Viral antigen was first detected by FA from 3 to 4 h PI. Approximately 75 to 80% of the cells contained antigen in the cytoplasm 9 to 11 h PI. The direct FA technique was found to be a sensitive method for detecting IPN virus in infected cells. Three strains of IPN virus were tested for serological cross reactions by FA and virus neutralization tests.


Serologic characterization of a Malacosoma disstria Hübner (Lepidoptera: Lasiocampidae) cell line.


Pathogenesis of aleutian disease of mink: nature of the antiglobulin reaction and elution of antibody from erythrocytes and glomeruli of infected mink. Erythrocytes from mink chronically infected with Aleutian disease virus (ADV) gave positive antiglobulin reactions with rabbit anti-mink immunoglobulin (Ig)G, anti-mink C3, and anti-mink serum, but did not react with anti-mink IgM. The strongest reaction was observed with anti-mink C3. Immunoelectrophoresis demonstrated that serum from rabbits injected with erythrocytes from ADV-infected mink gave a precipitin line with normal mink serum in the beta globulin region corresponding to C3. When normal mink erythrocytes were exposed to serum from ADV-infected mink, they were not sensitized, demonstrating that the antibodies in these mink sera were not directed against erythrocyte antigens. Glycine-hydrochloride buffer treatment of erythrocyte stromata and isolated glomeruli from ADV-infected mink yielded eluates containing serum proteins in the gamma globulin region which appeared to be IgG, and in the beta and alpha globulin regions which are probably complement components. In both erythrocyte and glomerular eluates, anti-ADV antibody was demonstrated. These findings suggested that the positive direct antiglobulin test and glomerulonephritis in Aleutian disease is due to the persistence of ADV and formation and deposition of ADV antigen-antibody-complement complexes on the erythrocyte surfaces and in glomerular capillaries.


An orthopantomographic study of prevalence of impacted teeth.


Hypogammaglobulinemia and thymic hypoplasia in horses: a primary combined immunodeficiency disorder. A severe combined immunodeficiency disorder was demonstrated in two Arabian foals which were full siblings. The defect in the B-lymphocyte system was shown by hypogammaglobulinemia, lymphopenia, and absence of germinal centers. The almost total absence of thymic tissue in one foal and the lack of thymic dependent lymphocytes in the spleens of both foals demonstrate a T-lymphocyte defect. In a retrospective study of total available Arabian foal cases, 4 of 15 had evidence of immunodeficiency.


Cellular fibrinolytic activity of human alveolar bone.


Allogenic demineralized dentin implants in jaw defects of Java monkeys.


A histologic study of experimentally induced radicular cysts.


Modes of healing histologically after endodontic surgery in 70 cases.


Correlation between histology and radiography in the assessment of healing after endodontic surgery.


Maxillary sinus and surgical movement of maxilla.


Age- and sex-dependence of the risk of intruding a root into the maxillary sinus.


Antifibrinolytic effect of Apernyl in "dry socket".


Radiographic criteria for the assessment of healing after endodontic surgery.


A follow-up study of 1,000 cases treated by endodontic surgery.


Cherubism.

Cherubism.


A histologic study of experimental oro-paranasal communications in monkeys.


A multivariate analysis of the influence of various factors upon healing after endodontic surgery.


A histobacteriologic study of dental and periapical structures after endodontic surgery.


Quantitative PCB standards for electron capture gas chromatography.


Cellular localization of carbonic anhydrase in avian tissues by labeled inhibitor autoradiography.


Maltose in total parenteral nutrition of rats.


Protein deficiency: contrasting effects on DNA and RNA metabolism in rat liver.


Primate pulp response to an ultraviolet light cured restorative material.


Effect of dietary protein and magnesium on selected ATP phosphotransferases and neuromuscular behavior in young rats.


An autopsy case of leiomyosarcoma of the maxilla.


Peripheral giant cell granulomas: an ultrastructural study of three lesions.


The pathogenesis of hydro-testis after ligation of the vas deferens: a study in several species.


The alpha cell in diabetic ketoacidosis.


Medical abortion in South Australia. A critical assessment of early complications.


Neglect after cingulectomy.

Neglect after cingulectomy.


Cerebrospinal fluid levels of IgG, IgA and IgM in neurologic diseases.


The release of TSH from organ cultures of human fetal pituitary glands.


16Alpha-hydroxylase activity in uterine tissue of the rhesus monkey.


The time course of recombination of HCG subunits observed by immunological methods and circular dichroism studies.


The effect of calcitonin on glucose assimilation and insulin secretion in man.


Subcellular distribution of glucuronyltransferase and its relevance for the enterohepatic circulation of oestrogens.


Phonophoresis as a new method for perfusion of the ovary in vivo.


Aromatization of 3H-pregnenolone sulfate in the rhesus monkey liver in vivo.


The application of synthetic LRF and TRF in a combined function test in patients with pituitary tumors.


Perforation in acquired diverticular disease of the terminal ileum.


Thymus dependency of cells involved in transfer of delayed hypersensitivity to Listeria monocytogenes in mice.


A review of the development of the health services of Rhodesia from 1923 to the present day.


Reversal by the MER tubercle bacillus fraction of the suppressive effects of heterologous antilymphocytic serum (ALS) on the allograft reactivity of mice.


Mitochondria and chloroplasts revisited.


Limbic projections to the thalamus.

Limbic projections to the thalamus.


Genotype and phenotype in human chromosome aberrations and in the minute mutants of Drosophila melanogaster.


Syntheses and biological activities of 7-ethyl-8-chloro-10-(1'-D-ribityl) isoalloxazine and 7-chloro-8-ethyl-10(1'-D-ribityl) isoalloxazine, analogs of riboflavin.


Antiasthma agents. 1. 4-Oxo-4H-(1) benzothieno-(3,2-b)pyran-2-carboxylic acid and 4-oxo-4H-(1)benzofuro(3,2-b)pyran-2-carboxylic acid.


Antiparasitic nitroimidazoles. 7. Some 4- and 5-styrylnitroimidazoles.