Interactions of retinol-binding protein with various chromophores and with thyroxine-binding protein. A model for visual pigments.


Monographs on fragrance raw materials.

Monographs on fragrance raw materials.


Toxicology: cost-time.

Toxicology: cost-time.


The relative susceptibility of four cephalosporins to a beta-lactamase from Bacillus cereus. The effects of purified beta-lactamase from Bacillus cereus on the activity of four cephalosporins are described. Drug inactivation was measured spectrophotometrically and confirmed by microbiological assay and by microbiological techniques involving determinations of minimum inhibitory concentrations and growth curves of Staphylococcus aureus NCTC 6571 (Oxford) in broth containing drug +/- enzyme. Cephaloridine was the most sensitive of the four cephalosporins, followed by cephalothin and the sodium salt of 7-cyanacetamidocephalosporanic acid, with cephalexin considerably resistant to inactivation. The possible use of this beta-lactamase in the sterility testing of cephaloridine and cephalothin is examined.


Social rank, observability, and sexual behaviour of rhesus monkeys (Macaca mulatta).


Letter: Lymphoma and anticonvulsive therapy.


Supraventricular arrhythmias caused by parenteral hyperalimentation.


Absence of concurrent permeability changes of intestinal mucosa in association with cholera toxin-induced secretion.


Letter: Asymptomatic gonorrhea in men.

Letter: Asymptomatic gonorrhea in men.


Protein nutrition and liver disease after jejunoileal bypass for morbid obesity.


Editorial: Somatostatin: newcomer from the hypothalamus.


Letter: Range of normal serum magnesium values.


Effect of thyrotropin releasing hormone (TRH) on the menstrual cycle in women.


Some thoughts on assessing the effectiveness of passive immunization to Rh. In 1000 Rh-negative women who are delivered of Rh-positive fetuses and receive passive immunity postpartum, there will be 5-10 failures to protect against sensitization.  Studies have been conducted to find out possible explanations for the failures and to devise means of preventing some or all of them.  Results from the investigations indicate that if all postabortal and postpartum patients at risk receive anti-D immune globulin and are carefully screened for large fetomaternal bleeds by the use of direct counting of fetal red cells in the  maternal circulation, the number of failures to protect against Rh sensitization can be reduced to an undiminishable minimum.


Amniotic fluid alpha-fetoprotein in anencephaly.


Evaluation of a latex agglutination test for amoebiasis.


Immunization of monkeys against a recently isolated human strain of Trypanosoma rhodesiense by use of gamma irradiation.


Antigenic variation in cyclically transmitted isolates of Trypanosoma gambiense.


Immunoglobulins in bovine trypanosomiasis.


Serum protein changes in trypanosomiasis in cattle.


Experimental Trypanosoma cruzi infections in Erthrocebus patas.


The capillary-tube agglutination (CA) test. A proposed aid for the diagnosis of trypanosomiasis in the field.


Serological studies on strains of Trypanosoma vivax.


Domestic risk factor--an attempt to assess risk of infection with Trypanosoma cruzi in houses in Brazil.


The host selection pattern and infection rates of Panstrongylus megistus in an area of eastern Brazil.


Isolation of a non-haemadsorbing strain of African swine fever (ASF) virus from a natural outbreak of the disease.


Experimental Sarcoptes scabiei infection in pigs: clinical signs and significance of infection.


Comparative studies on tomato aspermy and cucumber mosaic viruses. I. Physical and chemical properties.


Prenatal screening in the serodiagnosis of blood group sensitization.


Effect of heparin on the concentration of insulin in serum and plasma.


Pathology of macroglobulinemia--a review of Japanese cases.


Renal artery stenosis in childhood neurofibromatosis.


A cephalometric evaluation of the old Milwaukee brace and the modified Milwaukee brace in relation to the normal growing child.


Vaccination against Marek's disease with herpes virus of turkeys.


Blood vessels of the ciliary ganglion in man.


Outbreak of meningococcal disease in Devon. In a recent outbreak of 31 cases of meningococcal disease in Devon there were six deaths. Several patients had an unusual rash as the presenting feature and there was an unusually high incidence of complications, affecting the central nervous system, joints, and the heart among other sites.


Experience in monitoring gentamicin therapy during treatment of serious gram-negative sepsis. This paper reports our experience in monitoring gentamicin therapy during the treatment of 68 episodes of serious Gram-negative sepsis in 65 hospital patients. Most of the patients had major underlying disease. Of those who were adequately treated (peak serum concentrations of 5 mug/ml or more in 72 hours for septicaemia, urinary tract infection, and wound infection; and 8 mug/ml or more at some time during the course of treatment for pneumonia) 84% (46 out of 55) were cured. These serum concentrations could be achieved only by starting with a regimen of 5 mg/kg/day in three divided doses in all adult patients, subsequent dosage being determined by the results of rapid serum assay. The incidence of nephrotoxicity and symptomatic ototoxicity was no greater than in previous series. The main reason for assaying serum gentamicin is to ensure that an adequate dosage is achieved as soon as possible. In patients with impaired renal function or receiving prolonged high dosage assays also serve to guard against an excessive accumulation of gentamicin and an increased risk of toxicity.


Letter: Clinical diagnosis of Reye's syndrome.


Mechanism of the formation of concanavalin A-teichoic acid complexes.


Tissue distribution of coenzyme and other forms of vitamin B12 in control subjects and patients with pernicious anaemia.


Letter: Coagulation defects and arteriosclerosis in rabbits induced by diet containing triglycerides.


The possible site of action of sodium cromoglycate assessed by exercise challenge.


Familial thyroxine-binding globulin deficiency. A study of three Danish families.


Field studies on the use of coated porous polyurethane plugs as indwelling monitors of organochlorine pesticides and polychlorinated biphenyl contents of streams.


Evaluation of internal exposure to radionuclides of I, Cs and Co during maintenance operations on primary steam leak in a nuclear power station.


The fetal uptake of cerium-144-praseodymium-144 after injection of its chelates to pregnant rat.


Experimental amblyopia in monkeys. Further behavioral observations and clinical correlations.


On prognosis and treatment of acute severe pancreatitis with severe uremia.


Histological studies of the visual system in monkeys with experimental amblyopia.


Soybean protein flavor components: a review.


Fatty acid derivatives and glycolipids in high-protein bakery products.


Neurophysiologic and behavioral toxicologic testing to detect subclinical neurologic alterations induced by environmental toxicants.


Efferent connections of the red nucleus in the brainstem and spinal cord of the Rhesus monkey.


A combined fluorescence histochemical and electron microscopic method for studying special monoamine-containing cells (SIF cells).


Effect of oral alanine on blood beta-hydroxybutyrate and plasma glucose, insulin, free fatty acids, and growth hormone in normal and diabetic subjects.


Fluorescence histochemistry of monoamine-containing cell bodies in the brain stem of the squirrel monkey (Saimiri sciureus). IV. An atlas.


The local antibody response to R.S. virus infection in the respiratory tract. Nasopharyngeal secretions were taken during the acute phase of illness from 66 infants and children admitted to hospital with lower respiratory tract infections. Second secretions were taken, after an interval of 7 days, from 33 of these patients. A significant increase in neutralizing activity to R.S. virus was demonstrated in the nasopharyngeal secretions of patients in response to severe R.S. virus infection. Seventeen out of 25 patients (68%) with R.S. virus infections developed a rise in secretory neutralizing titre, compared with only 1 out of 8 patients (13%) with respiratory infections not involving R.S. virus.A high titre of secretory neutralizing activity was found more often in the acute phase of illness in patients with R.S. virus infections, especially bronchiolitis, than in patients with respiratory infections not involving R.S. virus. Fifteen out of 34 patients (44%) with R.S. virus bronchiolitis were found to possess a neutralizing titre of (1/4) or more in their first secretions, compared with 4 out of 12 patients (33%) with R.S. virus infections other than bronchiolitis and 3 out of 20 patients (15%) with respiratory infections not involving R.S. virus.A quantitative analysis of the immunoglobulins present in the secretions indicated that IgA was the only immunoglobulin consistently present at a detectable concentration. The geometric mean values of IgA, IgM and IgG in the secretions examined were found to be 22.3, 4.3 and 5.3 mg./100 ml. respectively.The neutralizing activity against R.S. virus, present in the secretions, was shown to be due to specific IgA antibody. This was accomplished by removing the neutralizing activity in two secretions by absorption with anti-IgA serum.


Veterinary medicine and surgery in the Republic of Vietnam.


The melena to admission time: a critical look at the patient with occult gastrointestinal bleeding.


Conservation surgery of the larynx and hypopharynx.


Interface of dental research, federal and civilian.


Acute epididymitis in Vietnam: review of 52 cases. The treatment experience of 52 military personnel in Viet Nam who had their 1st episode of acute epididymitis during a 9-month period in 1970-71 is reviewed.  75% of the patients were ages 18-30.  The right side was involved in 48.1% of cases and the left in 44.2%; the remaining 7 cases showed bilateral involvement.  Urine analysis revealed pyuria in 36 patients (69.2%).  White blood cells in urine were too numerous to count in 20 patients (38.5%).  Urine cultures were negative in all but 1 case.  The patients were hospitalized a median number of 9 days.  The therapeutic regimen consisted of absolute bed rest, elevation of the scrotum, and ice to the scrotum until tenderness disappeared.  Tetracycline was generally administered.  28 patients showed no obvious factor related to the etiology or pathogenesis of the epididymitis; however, 3 had gonorrheal urethritis on admission, 7 had had gonorrhea within 8 weeks of admission, 4 had had nongonococcal urethritis within the prior 8 weeks, and 6 had acute or subacute prostatitis on admission.  The paradox observed in this series between positive urinalasis and negative urine cultures may be explained by anaerobic and microaerophilic forms of the usual urinary pathogenic bacteria.  The frequency of recovery of pathogenic bacteria has been shown to be enhanced when anaerobic and microaerophilic cultures are performed.  Antibiotics thus assume an important role in the treatment of acute epididymitis, even if urine cultures are negative by the usual methods.  Further research is needed to confirm this hypothesis.


Case for diagnosis: a rapidly enlarging mass involving the right maxillary ridge.


The bus case: earliest contact military psychiatry.


An experimental evaluation of a therapeutic community.


Regional perinatal health centers.

Regional perinatal health centers.


Communication innovations at the mental hygiene consultation service-inpatient psychiatry interface.


A respirator development.

A respirator development.


Murine typhus in Vietnam.

Murine typhus in Vietnam.


Coronary artery disease: its impact on Naval and Marine Corps personnel.


The use of metronidazole in treating complications of amebiasis.


Chemotherapeutic results in a multi-drug resistant strain of Plasmodium falciparum malaria from Vietnam.


Pemphigus-like antibodies in sera of patients with thermal burns, gunshot wounds, and skin grafts.


Ultrasound and plantar warts: a double blind study.


Lobo's disease involving the skin of a dolphin.


Bradycardia tachycardia syndrome in a patient with a mitral prosthesis: case report.


Arteriovenous malformations in Koreans.

Arteriovenous malformations in Koreans.


Alcoholism clinic in a military setting: a combined disulfiram and group therapy outpatient program.


Isolated Crohn's disease of the appendix: case report.


Parenteral hyperalimentation for severe postvagotomy gastric stasis: case report.


Editorial: The hip osteotomy in orthopaedic surgery.


One stage diagnosis and treatment of intrathoracic disease.


Dosage compensation of sex-linked genes in Drosophila melanogaster. The activities of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in flies with normal and disturbed genetic balance.


Content of RNA polymerase in haploid and merodiploid strains of Escherichia coli.


The diagnosis of bovine mastitis: a critical evaluation of a polyvalent radial immunodiffusion test and other methods.


Locomotor body equilibrium after diazepam injection in the squirrel monkey.


Combined radiotherapy and chemotherapy in the treatment of Hodgkin's disease.


Use of serial exercise tests to assess the efficacy and duration of action of drugs for asthma.


Metabolism of synthetic steroids by animals and man.


Teratogenic screening methods and their application to contraceptive products.


U.S. Food and Drug Administration requirements for toxicity testing of contraceptive products.


Special requirements for plastic devices.


Design of early clinical trials for new contraceptive methods.


Special problems in toxicity testing of long acting depot contraceptives.


Special requirements for testing post-coital contraceptives.


Special requirements for testing spermicides.


Special requirements for toxicity testing of oral compounds administered continuously or cyclically.


Special requirements for toxicological testing of metal-releasing intrauterine devices.


Special toxicology for the prostaglandins.


Absorption and elimination of polychlorinated biphenyls (PCB) in goldfish.


Regional variation in the presence of mast cells in the mammalian brain.


On the phenomenon of the two linear Hammett relationships: its origin and its use as a tool in the analysis of diamine oxidase preparations.


Muscular subaortic stenosis and Friedreich's ataxia.


Placental CO2 transfer after fetal carbonic anhydrase inhibition.


Immunological identification of nascent subunits of wheat ribulose diphosphate carboxylase on ribosomes of both chloroplast and cytoplasmic origin.


Hyaluronidase and esterase activities of the venom of the poisonous brown recluse spider.


A rat liver protein with potential thrombin activity: properties and partial purification.


Mechanocardiography and hemodynamics.

Mechanocardiography and hemodynamics.


The similarities of optokinetic after-nystagmus to the vestibular nystagmus.


Quantitative determination of secretory immunoglobulin A (SIgA) in middle ear effusions.


Physiological correlates of auditory stimulus periodicity.


Structural and physiological features of the organ of Corti.


Facilitation of Fe(II) autoxidation by Fe(3) complexing agents.


Early diagnosis of arterial atherosclerosis by means of resonance electrosphygmography.


Hemodynamic stress and relief of the heart in the light of comparative physiological investigations.


The storage and manipulation of radiation dose data in a small digital computer.


Letter: A rapid method for the determination of tumour RBE.


Ossifying lipoma.

Ossifying lipoma.


Hypersensitivtiy responses in monkeys. VI. P-K, histamine, 48-80 responses after challenges with heterologous antigen or the other agents.


Dwarfism in the newborn: the nomenclature, radiological features and genetic significance.


Uptake of disodium cromoglycate in obstructive airways disease.


Allergy to snake venom: the use of radioimmunoassay for the detection of IgE antibodies against antigens not suitable for cutaneous tests.


Penicillin treatment of gonorrhoea in Edinburgh.


The effect of frequent administration of sodium cromoglycate to asthmatic children who previously responded poorly.


Absence of chlorinated dibenzodioxins and dibenzofurans from aquatic animals.


Polychlorinated biphenyls (PCB) in sea water and their effect on reproduction of Gammarus oceanicus.


An overview of the mycotoxicoses of domestic animals.


Proceedings: Serum amylase and related enzymes in diabetic ketoacidosis.


Superiority of simplified assay for folate with Lactobacillus casei ATCC 7469 over assay with chloramphenicol-adapted strain. Direct assay of serum folate by mixing serum with a mixture of L. casei and medium without autoclaving was found to provide serum folate results similar to those by extraction of serum with heat. This procedure was found more reliable than that using the same technique with chloramphenicol and a strain of L. casei adapted to grow in this material. A portion of apparent folate activity assayed in haemolysates by such direct assay was found to be due to material other than folate. It is recommended that heat extraction of haemolysates be continued for determination of whole blood folate, or that an additional standardization of technique be included if direct assay is used.


Echocardiographic M-scan technique in the diagnosis of pericardial effusion.


DEAE-cellulose chromatography: a method for dissociation of soluble immune complexes of hepatitis B antigen.


Reaction mechanisms of nascent C567 (reactive lysis). II. Killing of a rough form of Escherichia coli by C567, C8, and C9.


An immunologic model in laboratory animals for the study of Neisseria gonorrhoeae.


Characterization of brain-associated rabies neutralizing substance.


Pharmacokinetics of ampicillin in the newborn infant.


Protective effect of pyocin against lethal Pseudomonas aeruginosa infections in mice.


The effect of chemotherapy for tuberculosis on immunity to tuberculosis.


On the control of cholesterol synthesis.


Management of stable angina.

Management of stable angina.


Microbes. 4. Robert Koch: anthrax bacilli isolated.


Studies in head injury. I. An experimental model of closed head injury.


Studies in head injury. II. Effect of dexamethasone on traumatic brain swelling.


RNA synthesis in the uterine epithelium stimulated by estrogen injection.


The synthesis of fat and water soluble arseno organic compounds in marine and limnetic algae.


Uptake and hydrolysis of 131-I-albumin in the toad liver. The effect of low body temperature.


Cultivation of animal cells in chemically defined media, a review.


Isoelectric fractionation of sulfated glycoproteins on polytetrafluoroethylene-coated columns.


Specific tlc, gc, and gc-ms methods for kinetic studies with COMT.


Flexibacteria.

Flexibacteria.


Differentiation of DNA and RNA on filter paper discs.


Amidase activity of human alpha-macroglobulin.


Inhibition of growth of transplantable melanotic melanoma in golden hamsters (Mesocricetus auratus, Waterhouse) by chlorpromazine.


Inhibiting effect of chloroquine diphosphate on the growth of tumors and activity of some acid hydrolases of a melanotic melanoma in golden hamsters (Mesocricetus auratus, Waterhouse).


A new case of IgD-myeloma associated with hypogammaglobulinemia.


Evaluation of lidoflazine as an anti-anginal drug.


Reaching in light and dark after unilateral posterior parietal ablations in the monkey.


An international survey on nutrition and pancreatitis.


Developmental studies on some enzymes associated with 'detoxication'.


Careful presentation of evidence helps clear hospital.


The CHSLP financial model.

The CHSLP financial model.


The use of a whole body counter for measuring bone marrow erythroid activity by profile scanning.


Recent advances in whole-body counting: a review.


Effects of microsporidan infection on larval trematodes: infection with Nosema strigeoideae or N. echinostomi.


The etiological agent of cytoplasmic incompatibility in Culex pipiens.


The infection of nonmosquito hosts by injection with spores of the microsporidan Nosema algerae.


High energy x-ray therapy of gastric carcinoma.


An electron microscopic study of the neurons of the primate motor and somatic sensory cortices.


An electron microscope study of the termination of afferent connections to the primate motor cortex.


Preparation and use of benzhydrylamine polymers in peptide synthesis. II. Syntheses of thyrotropin releasing hormone, thyrocalcitonin 26-32, and eledoisin.


The importance of routine urine culture in malnourished children.


Nutritional problems of children in new urban families.


Adrenal regulation of protein synthesis in protein malnutrition.


Pediatric surgery. 1.

Pediatric surgery. 1.


Advantages and limitations of radioisotope clearance methods from a clinician's point of view.


Renal clearance investigation with radionuclides.


Aphakia and multiple ocular defects in Saint Bernard puppies.


Content of beta-2-microglobulin and albumin in human amniotic fluid. A study of normal pregnancies and pregnancies complicated by haemolytic disease.


Present day management and treatment of Hodgkin's disease.


Isolation and identification of Corynebacterium vaginale (Haemophilus vaginalis) in women with infections of the lower genital tract.


Effect of oral contraceptives on the plasma concentration of pyridoxal phosphate.


Multiple fetal and adult hemoglobins in Malaysian Macaca nemestrina: consequences of a-chain variation.


Amino acid compositions of the tryptic peptides comprising the beta-hemoglobin chain of Macaca nemestrina.


Intestinal absorption of D-xylose and folic acid in protein-deficient patas monkeys (Erythrocebus patas).


Sharing of Cercarienhüllen Reaktion antigens between Schistosoma mansoni cercariae and adults and uninfected Biomphalaria pfeifferi.


Ecology of viruses isolated from Panamanian phlebotomine sandflies.


Childhood pigeon breeder's disease.

Childhood pigeon breeder's disease.


Simian gastropathy with submucosal glands and cysts. Gastritis glandularis or cystica profunda?


Isolation of Candida species from the gastrointestinal tract in malnourished children.


Behavior of an all-juvenile group of Rhesus monkeys.


Effects of nitroglycerin on left ventricular wall motion in coronary artery disease.


The analysis of the effects of early experiences.


Dynamics of spermiogenesis in Drosophila melanogaster. 3. Relation between axoneme and mitochondrial derivatives.


Comparative growth characteristics of VERO cells on gas-permeable and conventional supports.


DNA synthesis following refeeding of starved Tetrahymena pyriformis GL: starved cells are arrested in G 1.


A convenient method for enucleating cells in quantity.


Perivascular hemorrhagic lesions in temporal cortex following cerebral infarction (a morphological study).


The chemical composition and ultrastructure of the cell wall of Scenedesmus obliquus. II. Amino acids, proteins and antigens.


Comparison of the tissue receptors for Vibrio cholerae and Escherichia coli enterotoxins by means of gangliosides and natural cholera toxoid. The in vitro binding properties of enterotoxins of Vibrio cholerae and Escherichia coli to different pure gangliosides and related neutral glycosphin-golipids were analyzed with a sorbent assay utilizing plastic tubes to which the glycolipid substances had been coupled. It was found that the cholera toxin bound to G(M1) ganglioside better than to the other tested substances G(M3), G(M3)-NGN, G(M2), G(D1a), G(D1b), G(T), G(A1), tetrahexoside-GlcNac and globoside. With this assay using G(M1)-coated tubes it is possible to measure cholera toxin even at concentrations below 1 ng/ml. Also enterotoxin of various E. coli strains bound to G(M1), but the affinity was much less than for cholera toxin. The G(M1) ganglioside, in contrast to the other glycosphingolipids, effectively inactivated cholera toxin as determined with the intradermal and the ileal loop assays; approximately equimolar concentrations of the ganglioside in relation to toxin sufficed. Also, the skin and ileal loop activities of E. coli enterotoxins could be inhibited by G(M1); however, several orders more of the ganglioside were required for such inhibition than for inactivation of the cholera toxin, and the differences between G(M1) and the other substances were less pronounced for E. coli toxins. Preincubation of rabbit ileal loops with choleragenoid, a natural toxoid of V. cholerae which has binding properties to the G(M1) ganglioside similar to cholera toxin, made the loops resistant to subsequently added enterotoxin of V. cholerae. The responsiveness to enterotoxin of E. coli was not reduced by this toxoid. A likely interpretation of these data is that the G(M1) ganglioside constitutes or at least contains the structure of functional tissue receptors for the cholera toxin, whereas the weak binding to G(M1) by E. coli enterotoxins is probably a pathogenetically insignificant reflection of structural similarities between these toxins and cholera toxin. Consequently, the cholera toxoid by occupying functional intestinal G(M1) receptors for the cholera toxin could inhibit the ileal response to this toxin, but not the response to E. coli enterotoxin since the intestinal receptors for the latter toxin are not affected by the cholera toxoid.


Pathogenicity and immunogenic efficacy of a live attentuated plaque vaccine in vervet monkeys. A live attenuated Yersinia pestis (Pasteurella pestis) vaccine strain designated EV51f, which had been passaged through guinea pigs previously treated with ferrous sulfate, was shown to be pathogenic for African green vervet monkeys (Cercopithecus aethips pygerythrus), but not for guinea pigs. The bacilli multiplied in the monkeys, as shown by positive blood cultures, caused an elevation of white cell counts and rectal temperatures, and resulted in death of 26% (13/50) of animals. Postmortem findings of these animals were typical of bubonic-septicemic plague. This vaccine did not cause deaths in 50 guinea pigs even in doses up to 100 million viable bacilli inoculated subcutaneously. It is suggested that the virulence of an attenuated Y. pestis strain which does not produce pigment on a defined medium containing hemin, but possesses all other known virulence determinants, is dependent on the availability of iron in vivo. The serological response of the monkeys as determined by the hemagglutinating and mouse protective antibodies was high one month after vaccination and also in guinea pigs, as shown by virulent challenge. This antibody level declined in monkeys over a period of nearly 6 months, and a decline in immunity was confirmed by virulent challenge which resulted in the death of 30% of vaccinated monkeys. The level of immunity in monkeys did not appear to be related to the dose of vaccine.


Contact sensitivity in vitro: activation of actively allergized lymphocytes by a beryllium complex.


Pathogenesis and immunology of experimental gonococcal infection: virulence of colony types of Neisseria gonorrhoeae for chicken embryos. The virulence of gonococcal strains and colony types was evaluated in embryonated hen eggs of various ages inoculated by different routes. Striking differences in virulence of colony types were revealed by intravenous inoculation of 11-day embryos. T1 and T2 colony types were found to have high virulence for embryos, whereas T3 and T4 colony types were relatively avirulent. These observations are in accord with previous studies in volunteers. The differences in virulence were not related to differences in toxicity of killed gonococci, sonic lysates, or to the susceptibility of gonococci to cidal effects of chicken embryo blood. Rather, they appeared to involve differences in clearance of gonococci from the blood stream and subsequent multiplication of the virulent colony types. Infection with virulent colony types appears to be primarily bacteremic in this model. Preliminary experiments indicated that chicken embryos may be protected against the lethal infection by prior treatment of the inoculum with normal and immune rabbit serum. This protective effect was not associated with bactericidal activity. The chicken embryo model is potentially useful as a means of investigating attributes of virulence of gonococci and factors in immunity against gonorrhea.


Immunological studies on the envelope component of Venezuelan equine encephalomyelitis virus. Treatment of purified Venezuelan equine encephalomyelitis virus with the nonionic detergent Triton X-100 permitted spearation of the envelope from the core component. The isolated envelope was a noninfectious immunogen which reacted in hemagglutination, hemagglutination inhibition, complement fixation, and neutralization serological reactions.


Structure and biological functions of human IgD. 3. Phylogenetic studies of IgD.


Isolation of a low molecular weight ragweed pollen allergen: Ra5.


A study of immunoglobulins in jejunal secretion and serum of patients suffering from Mediterranean cirrhosis.


Regulation of lipogenesis and the total activities of lipogenic enzymes in a primary culture of hepatocytes from prenatal and early postnatal chicks.


Purification of ribonucleic acid polymerase from SP82-infected Bacillus subtilis.


In vivo and in vitro transcription by ribonucleic acid polymerase from SP82-infected Bacillus subtilis.


Recognition of primary sequence variations among sperm whale myoglobin components with successive proteolysis procedures.


A mammalian endonuclease specific for apurinic sites in double-stranded deoxyribonucleic acid. I. Purification and general properties.


Preparation and prolonged tissue retention of liposome-encapsulated chelating agents.


Identification of blood meals of sandflies (Diptera: psychodidae).


Three isoaccepting forms of leucyl transfer RNA in mitochondria.


Cerebral circulation and metabolism. Summary of International Symposium, June 6-10, 1973.


Long term effects of aflatoxin B1 and viral hepatitis on marmoset liver. A preliminary report.


Ultrastructural changes in the liver of baboons following lead and endotoxin administration.


Mechanism of denaturation of haemoglobin by alkali.


Intra- and interpopulation selection concerning the alcohol dehydrogenase locus in Drosophila melanogaster.


Therapeutic value of local perfusion for acute spinal cord trauma.


Nitrosocarbaryl as a potent mutagen of environmental significance.


In vivo measurement of cerebral glucose metabolism employing 11C-labeled glucose.


Impaired tool-using behavior in monkeys from bilateral destruction of the dorsomedial nuclei of the thalamus.


Clinical grade, regional cerebral blood flow, and angiographical spasm in the monkey after subarachnoid hemorrhage.


The effect of chronic cerebellar stimulation upon epilepsy in man.


Magnocellular neuroendocrine cells in the monkey hypothalamus.


The modification of L-dopa induced dyskinesias in the monkey by unilateral nigral lesions.


Experimental tremor following CNS lesions in monkeys.


Physiopathological study in monkeys with partial and complete destruction of cerebellar structures.


Some new observations on intracerebellar nuclei: their participation in patterned movements.


"Triadic" synapses and other articulations of interneurons in the lateral geniculate nucleus of rhesus monkeys.


Petit mal status presenting as psychasis.


Cerebellopontine influence on the motor system: a functional and anatomical study following section of the brachium pontis in trained macaque monkeys.


Visual inhibition of nystagmus by the flocculus.


Abnormal drug metabolism in rats after an inflammatory insult.


The prophylactic and therapeutic effects of gold sodium thiomalate against adjuvant-induced polyarthritis in rats.


Esophageal surgery: a procedure for posterior invagination esophagogastrostomy in one-stage without positional change.


Surgical management of carcinoma of the maxilla.


Causes of death in irradiated adult insects.


Urinary excretion in the rat of nifurpipone (NP) and of nitrofurantoin (NTF) administered by different routes.


Response pattern complexity of auditory cells in the cortex of unanesthetized monkeys.


The inactivation by carbenoxolone of individual human pepsinogens and pepsins.


Transfer factor--fact or fiction?

Transfer factor--fact or fiction?


Restraint and anesthesia in zoo animal practice.


GM1-gangliosidosis. I. Clinical aspects and biochemistry.


Effect of diphenylhydantoin on insulin response in the oral glucose tolerance test in children and adolescents.


Sorbitol in total parenteral nutrition in pediatric patients.


Pentastoma larvae in a squirrel monkey.

Pentastoma larvae in a squirrel monkey.


Persistent pupillary membranes in a rhesus monkey.


Candida albicans infections associated with antibiotic and corticosteroid therapy in spider monkeys.


Pulmonary pathology in stillborn nonhuman primates.


Detection of isoniazid in urine and blood serum of nonhuman primates.


Alpha-ketoglutaric semialdehyde dehydrogenase of Pseudomonas. Properties of the separately induced isoenzymes.


An alpha-D-galactosyl-binding lectin from Bandeiraea simplicifolia seeds. Isolation by affinity chromatography and characterization.


Nutritional study of three strains of Naegleria gruberi.


Kudoa cerebralis sp. n. (Myxosporidea, Chloromyxidae) from the striped bass, Morone saxatilis (Walbaum).


Microsporidian parasites of trematode larvae from aquatic snails in West Malaysia.


Three avenues to ecologic research on parasitic protozoa.


Rumen ciliate fauna of Alaskan moose (Alces americana), musk-ox (Ovibos moschatus) and Dall moutain sheep (Ovis dalli).


Pollution.

Pollution.


Effects of PCB and DDE in cormorants and evaluation of PCB residues from an experimental study.


Reproductive failure and mortality in mink fed on Great Lakes fish.


Obstruction of the common bile duct. Duodenal diverticula.


The role of pheromones in the social communication of marmoset monkeys (Callithricidae).


On the fine structure of the acidophilic hot-spring alga Cyanidium caldarium: a taxonomic approach.


Feto-maternal production and transfer of cortisol in the rhesus (Macaca mulatta).


Quantification of 20alpha- and 20beta-dihydroprogesterone in plasma of the pregnant rhesus monkey.


A comparison of vocalizations of red-tailed monkeys and blue monkeys, Ceropithecus ascanius and C. mitis, in Uganda.


Beneficial electrophysiologic effects of nitroglycerin during acute myocardial infarction.


Nitroglycerin-induced reduction in the incidence of spontaneous ventricular fibrillation during coronary occlusion in dogs.


Ocular side-effects of oral contraceptives.


Minimizing systemic infection during complete parenteral alimentation of small infants.


Effects of intensity on the phototactic responses of adult anuran amphibians: a comparative survey.


Electric birefringence of native DNA in an alternating field.


Letter: Acute pancreatitis and diabetic ketoacidosis in hypothermia.


Rifampicin: a first line tuberculostatic drug. A critical review and appraisal.


Standardization of diagnostic materials. 5. Tissue culture media and reagents. The various factors that affect the growth of cell cultures in vitro are considered and suggestions are made as to where the standardization of components would help to provide more uniform products. The manufacture of powdered media in bulk and the establishment of cell banks have been major advances, but much research is needed for the standardization or replacement of serum.


A controlled field trial of a serogroup A meningococcal polysaccharide vaccine. A controlled field trial of a serogroup A meningococcal polysaccharide vaccine was conducted at three locations in Egypt during the winter cerebrospinal meningitis (CSM) season of 1971-72. The study population consisted of schoolchildren 6-15 years of age. No cases of serogroup A meningococcal CSM occurred in the group of students vaccinated with the test vaccine whereas 8 cases occurred in the control group vaccinated with tetanus toxoid, and 151 cases occurred in an unvaccinated contrast group. The case rate was significantly different between the test and control groups as well as between the test and contrast groups but was similar between the control and contrast groups. The previously demonstrated safety of the vaccine was confirmed. A significant serological response was elicited in the majority of the vaccinated students.


Polymer enhancement of automated immunological nephelometric analysis, as illustrated by determination of urinary albumin.


Transport of 5-methyltetrahydrofolic acid into the cerebrospinal fluid in man.


Genetic control of quantitative variation in self-incompatibility proteins detected by immunodiffusion. Single radial immunodiffusion was used to study the self-incompatibility (S) proteins present in stigmas of two inbred lines of Brassica oleracea: a self-incompatible line and a self-compatible mutant derived from it. The genetic basis of observed quantitative differences in S proteins between the two inbreds was shown to be determined by a single gene with dosage effects. Self-pollination of individual plants with high, intermediate and low levels of S protein in the stigmas, respectively, resulted in low, intermediate and high seed set.


A new mutant controlling mitotic chromosome disjunction in Drosophila melanogaster. A new mutant, mit (mitotic loss inducer), is described. The mutant is recessive and maternal in action, producing gynandromorphs and haplo-4 mosaics among the progeny of homozygous mit females. Mosaic loss of maternal or paternal chromosomes can occur. The probabilities of either maternal or paternal X chromosome loss are equal. mit has been mapped to approximately 57 on the standard X chromosome map.-Using gyandromorphs generated by mit, a morphogenetic fate map, placing the origins of 40 cuticular structures on the blastoderm surface, has been constructed. This fate map is consistent with embryological data and with the two other fate maps generated in different ways.


Immunogenetic aspects of nasopharyngeal carcinoma. II. Analysis of ABO, rhesus and MNSs red cell systems.


Prospects for the prenatal diagnosis of familial hypercholesterolemia.


Binding of metabolites of dietary 4-dimethyl-aminoazobenzene and 2-methyl-4-dimethylamino-azobenzene to rat liver DNA and protein of subcellular fractions.


The effect of phenobarbital on cholesterol gallstones in hamsters. This experimental study was undertaken to follow the influence of phenobarbital on bile chemistry and gallstone formation. Phenobarbital (400 mg/kg/day) was administered to golden hamsters receiving a diet known to induce cholesterol gallstones. After a 28-day period none of the control animals had gallstones whereas five of 12 animals with added phenobarbital developed stones. Ten of 19 animals on the lithogenic diet formed gallstones but when phenobarbital was added gallstones occurred in 16 of 19 hamsters. The administration of phenobarbital resulted in the production of bile which was relatively more saturated with cholesterol, the bile salt + phospholipid: cholesterol of 24.8 +/- 12.7 in control animals altering to 9.1 +/- 6.2 in animals receiving phenobarbital. Hepatic bilirubin uridine diphosphate-glucuronyl transferase activity was not increased in animals on phenobarbital. It was concluded that, in the hamster at least, there is no indication that large doses of phenobarbital will reduce the potential for gallstone formation.


Low dosage anti-D immunoglobulin in the prevention of rhesus isoimmunization.


Periodontal traumatism produced by sustained increase in occlusal vertical dimension: a histopathological study.


Demonstration by placebo response in asthma by means of exercise testing.


Positive Foley catheter tip cultures--fact or fancy?


Isolation of human and porcine islets of Langerhans and islet transplantation in pigs.


Editorial: Prostaglandins--tour d'horizon.


Analysis of selected characteristics of a matched sample of nonprofit and proprietary nursing homes in the state of Washington.


Evolution in action.

Evolution in action.


Reduction in brain dopamine following experimental cerebral ischaemia.


Vaccination of rhesus monkeys against malaria by use of sucrose density gradient fractions of Plasmodium knowlesi antigens.


Blue-green bacteria synthesise L-tyrosine by the pretyrosine pathway.


Vitamin B12 antagonism by monocarboxylic acids and anilides of cyanocobalamin.


Human thyroxine-binding globulin (TBG) solid phase radioassay.


Circulating antibodies and leucocyte migration inhibition to colon-associated antigen in ulcerative colitis and Crohn's disease.


Tuberculosis in a domesticated Asiatic elephant Elephas maximus.


Bacterial colonial microstructure.

Bacterial colonial microstructure.


Effect of esters of p-hydroxybenzoic acid on spheroplasts of Serratia marcescens and protoplasts of Bacillus megaterium.


Ferulic acid as the inductor of messenger RNA synthesis related to laccase formation in the wood rotting fungus Pleurotus ostreatus.


Miliary tuberculosis.

Miliary tuberculosis.


Aspergillus species endocarditis. The new face of a not so rare disease.


Methods to determine CO2 levels in a gonococcal transport system (Transgrow).


The post-mortem microbiological analysis as an indicator of nosocomially-significant microorganisms in the hospital environment.


A five-year inhalation study with natural uranium dioxide (UO2) dust. II. Postexposure retention and biologic effects in the monkey, dog and rat.


Childhood acromegaly: successful therapy with conventional radiation and effects of chlorpromazine on growth hormone and prolactin secretion.


Transport of thyroid hormones in serum and cerebrospinal fluid.


Thyrotropin and thyroidal responses to consecutive doses of thyrotropin-releasing hormone.


Growth hormone, thyrotropin, and prolactin responses to thyrotropin-releasing hormone following diethylstilbestrol pretreatment.


Immunogenicity and partial purification of soluble H-2 antigens extracted with hypertonic salt.


Specific binding of mouse IgE with rat mast cells.


Effect of anti-mu-specific immunosuppression on Friend virus leukemia.


Participation of different cell populations in antigen- and mitogen-induced lymphocyte proliferation. Evidence that blastogenic factor-sensitive lymphocytes are inactivated by 5-bromo-2'-deoxyuridine.


Aggregation of Fc'mu subfragments produced by tryptic hydrolysis of human IgM at elevated temperature.


Forssman-containing liposomes: complement-dependent damage due to interaction with a monoclonal IgM.


Skin glands as organs of communication in mammals.


Comparative chemistry of sebum.

Comparative chemistry of sebum.


Letter: Electron microscopic visualization of the folded chromosome of Escherichia coli.


Letter: Crystallography of Bacillus subtilis levansucrase.


Synthesis and turnover of Euglena gracilis mitochondrial DNA.


Proceedings: Role of cerebellum in prism adaptation.


Acid protease activity in Plasmodium falciparum and P. knowlesi and ghosts of their respective host red cells.


Part II. The spinal cord injured patient.


Special needs of long-term patients.

Special needs of long-term patients.


Cerebrospinal fluid pulsatile force and focal ventricular dilatation in cases of growing skull fracture.


Characterization of echovirus types 7, 12, and 29 hemagglutinins for human and monkey erythrocytes.


The prevention of radiation-induced, early, transient incapacitation of monkeys by an antihistamine.


Effects of extracellular proteases on incorporation of amino acids into protein by isolated rat tissues.


Inhibition of human platelet-collagen adhesion reaction by amitriptyline and imipramine.


Studies on the red cell and antibody-reactive sites of the parvovirus H-1: effect of fixatives.


The role of demethylbetahistine in the depressor response to betahistine in the rat.


Strain specificity of monodisperse gamma-globulin appearance after immunization of inbred mice.


Paget's disease of bone. Experience with a diphosphonate (disodium etidronate) in treatment.


An anatomico-physiological principle governing the direction of the gastro-intestinal mucosal folds during life.


Carbenicillin-induced coagulopathy.

Carbenicillin-induced coagulopathy.


A new, highly susceptible laboratory animal for Chagas's disease.


American trypanosomiasis: a new host.

American trypanosomiasis: a new host.


Infections with Plasmodium falciparum and Plasmodium vivax in the owl monkey--model systems for basic biological and chemotherapeutic studies.


The trypanocidal action of human serum and of baboon plasma.


Letter: Sarcocystis in the Malaysian long-tailed monkey, Macaca irus.


Letter: Malarial relapse mechanism.

Letter: Malarial relapse mechanism.


Sarcosporidiosis in man.

Sarcosporidiosis in man.


Proceedings: Attempted infection of chimpanzees and cats with Sarcocystis of cattle.


Arbovirus studies in Nupeko forest, a possible natural focus of yellow fever virus in Nigeria. I. Description of the area and serological survey of humans and other vertebrate hosts.


Proceedings: An infection of Klossiella muris Smith and Johnson, 1902, in a wild Mus musculus from Iran.


Experimental Trypanosoma cruzi infection in rhesus monkeys--the acute phase.


Proceedings: Culture of Schistosoma haematobium and the effects of immune sera in vitro.


Chemical and physicochemical properties of two strains of tobacco streak virus.


LambdaattB-attP, a lambda derivative containing both sites involved in integrative recombination.


Selective inhibition of viral protein accumulation in interferon-treated cells; nondiscriminate inhibition of the translation of added viral and cellular messenger RNAs in their extracts.


Chromatic information processing in the foveal projection (area striata) of unanesthetized primate.


Effects of selective coronary hypotension and nitroglycerin or Bay a 1040 on the distribution of Rb86 clearance in the canine heart.


Effects of thyrotropin-releasing hormone (TRH) in maternal, fetal and newborn rats.


The effect of caloric deprivation upon thyroid function in the neonatal rat.


Role of glucose as a regulator and precursor of amino acids in the pancreatic beta-cells.


Aromatization by the immature rhesus monkey ovary.


Cone mechanisms in the electroretinogram of the cynomolgus monkey.


Experimental ocular irradiation with accelerated protons.


Gargoylism--Hurlers syndrome.

Gargoylism--Hurlers syndrome.


Citrate-Mg2+ transport in Bacillus subtilis. Studies with 2-fluoro-L-erythro-citrate as a substrate.


Studies on human beta-D-N-acetylhexosaminidases. 3. Biochemical genetics of Tay-Sachs and Sandhoff's diseases.


Generation of superoxide free radical during the autoxidation of thiols.


Characterization of the binding protein for epidermal growth factor.


Determination of the structure and conformation of bacterial polysaccharides by carbon 13 nuclear magnetic resonance. Studies on the group-specific antigens of Neisseria meningitidis serogroups A and X.


Hetero-implants of epiphyseal cartilage in fracture gaps.


Immunological studies on the acidic chromogranins and on dopamine beta-hydroxylase (EC 1.14.2.1) of bovine chromaffin granules.


Temperature-sensitive mutations affecting the regenerative sodium channel in Drosophila melanogaster.


Lactose loading test on protein calorie malnutrition.


Clinical uses of central venous cannulation.


Scattered radiation dosage as a function of x-ray energy.


Comparison of electron beams from the Siemens betatron and the Sagittaire linear accelerator.


Physical characteristics of fast-neutron beams from a compact medical cyclotron.


Compression osteosynthesis of lateral malleolar fractures with a figure-of-eight cerclage.


Studies on Crohn's disease. 3. Concentrations of immunoglobulins G, A, and M in the mucosa of the terminal ileum.


Effects of methoxyflurane and two metabolites on sodium transport in the toad bladder.


Mycetoma formation in Trichophyton rubrum infection.


Adjuvant long-term chemotherapy in complex treatment of operable breast cancer.


Placental lactogen levels in rhesus isoimmunization. A prospective study of the plasma levels of human placental lactogen (HPL) in pregnancies complicated by rhesus isoimmunization showed that in mild and moderately affected cases the levels were normal, while in severely affected cases they were raised. Serial levels of HPL before the 26th week provide a valuable indication of fetal outcome, and we suggest that this estimation should be used routinely as an adjunct to other tests in the management of rhesus isoimmunization.


Attempts to demonstrate "tumor-globulin" in sera of patients with malignant neoplasms.


Reversible increase in pulmonary vascular permeability after Pseudomonas aeruginosa bacteremia in unanesthetized sheep.


Acrosomal proteinase inhibitors in rhesus monkey (Macaca mulatta) oviduct fluid. Acrosomal proteinase inhibitors were found in rhesus monkey fallopian tubes.  Spermatozoa contain a proteolytic enzyme in their acrosomal heads and which would be adversely affected by the presence of acrosomal proteinase inhibitors.  The inhibitor concentration was high before ovulation, decreased to low levels around the time of ovulation, and rose to high levels again within 1-3 days after ovulation.  Alpha-1-antitrypsin, alpha-2-macroglobulin and alpha-1-antichymotrypsin all followed this high-low-high pattern.  Alpha-1-antitrypsin in human cervical mucus is found to follow a similar waxing and waning according to progesterone secretion.


Uterine bleeding in the rhesus monkey in response to various designs of intrauterine devices. To study quantitative menstrual blood loss (MBL), 10 normally rhesus monkeys and 18 ovariectomized, hormonally supplemented monkeys (estradiol and progesterone) were inserted with IUDs: a silastic cylinder and 3 modifications the polyethylene  Tatum T device.  A 92% increase (p.01) in MBL was noted in the Y-T device monkeys (n=7) and a 70% increase (p.05) in the large oval T ones (n=5).  MBL was unaffected by the small oval T, and by the cylindrical Silastic devices after the second postinsertion period.  Intermenstrual bleeding was observed in 4 of 7 Y-T device animals but was uncorrelated with MBL.  It was concluded that rhesus monkeys may serve as good research models for designing human IUDs.


Oral contraceptive activity of danazol in the rhesus monkey.


Functional morphology of the lining membrane of the middle ear and Eustachian tube: an overview.


Physiological genetics of melanotic tumours in Drosophila melanogaster. VII. The relationship of dietary sterols to tumour penetrance.


The role of mycoplasma infection in human reproductive failure.


A case for the abolition of long stay hospitals and beds.


Ejaculation by male rhesus in the absence of female partners.


Use of an elemental diet for intestinal disorders and for the critically ill.


Glycogen synthetase and phosphorylase activities in different tissues of genetically obese mice.


Pituitary function in malnutrition.

Pituitary function in malnutrition.


Diverticulosis of the appendix: a collective review.


Treatment of functional hypothyroidism by oral TRF monitored by daily urinary thyroxine and histamine assay.


Bioassay and immunoassay measurements of serum thyrotropin in primary hypothyroidism before and after the administration of thyrotropin releasing hormone.


Genetic marker systems in Arctic populations. II. Polymorphism of C'3 in Finnish Lapps.


Frequency of Gm, Inv and ISf phenotypes in the population of 4 Yugoslavian villages.


Carbonic anhydrase II polymorphism in Africa.


B and T lymphocyte subpopulations in human peripheral blood.


ABO and Rh blood groups in relation to sex ratio of sibs.


Technique for obtaining pure endometrial fluid from rhesus monkeys.


Nonlethal deciliation of Tetrahymena by a local anesthetic and its utility as a tool for studying cilia regeneration.


Histone F1 of Tetrahymena macronuclei: unique electrophoretic properties and phosphorylation of F1 in an amitotic nucleus. Histone fraction F1 has been isolated and purified from macronuclei of the ciliated protozoan, Tetrahymena pyriformis. In many respects, Tetrahymena F1 is similar to that of other organisms. It is the only Tetrahymena histone soluble in 5% perchloric acid or 5% trichloroacetic acid, has a higher molecular weight than any other Tetrahymena histone, is the histone most easily dissociated from Tetrahymena chromatin, and is susceptible to specific proteolytic cleavage. However, unlike F1 in all other organisms, Tetrahymena F1 is not the slowest-migrating histone fraction when analyzed by polyacrylamide gel electrophoresis at low pH. Tetrahymena F1 also exhibits unusual behavior in sodium dodecyl sulfate-containing polyacrylamide gels, migrating faster than calf thymus F1 at pH 10, and slower than calf thymus F1 at pH 7.6. Tetrahymena F1 was found to be highly phosphorylated in rapidly growing cells, suggesting that the relationship between cell replication and F1 phosphorylation previously observed in mammalian cells may extend to all eukaryotes. The observation that extensive F1 phosphorylation occurs in macronuclei, which divide amitotically, argues against a unique role for F1 phosphorylation in the process of chromosome condensation at mitosis.


The effect of the concentration of golden hamster spermatozoa on the acrosome reaction and egg penetration in vitro.


Genetic control of mitochondrial thymidine kinase in human-mouse and monkey-mouse somatic cell hybrids. Distinctive thymidine (dT) kinase molecular forms are present in mouse, human, and monkey mitochondria. Disk polyacrylamide gel electrophoresis (disk PAGE) analyses have shown that the mitochondrial-specific dT kinases differ from cytosol dT kinases in relative electrophoretic mobilities (Rm). Furthermore, the mouse mitochondrial dT kinase differs in Rm value from primate mitochondrial dT kinases. The mouse and primate cytosol dT kinases can also be distinguished. Disk PAGE analyses have been carried out on the cytosol and mitochondrial dT kinases of human-mouse (WIL-8) and monkey-mouse (mK.CV(III)) somatic cell hybrids in order to learn whether the mitochondria of the hybrid cells contained murine mitochondrial-specific, primate mitochondrial-specific, or both dT kinases. WIL-8 cells were derived from cytosol dT kinase-negative, mitochondrial dT kinase-positive mouse fibro blasts and from cytosol dT kinase-positive, mitochondrial dT kinase-positive human embryonic lung cells; they contained mostly mouse chromosomes and a few human chromosomes, including the determinant for human cytosol dT kinase. The mK.CV(III) cells were derived from cytosol dT kinase-negative, mitochondrial dT kinase-positive mouse kidney cells and from cytosol dT kinase-positive, mitochondrial dT kinase-positive monkey kidney cells; they contained mostly mouse chromosomes and a few monkey chromosomes, including the determinant for monkey cytosol dT kinase. Disk PAGE analyses demonstrated that the mitochondria of human-mouse and monkey-mouse somatic cell hybrids contained the mouse-specific mitochondrial dT kinase but not the human- or monkey-specific mitochondrial dT kinase. These findings suggest that primate cytosol and mitochondrial thymidine kinase genes are coded on different chromosomes.


Biopsy of enlarged, superficial lymph nodes.


Editorial: Pancreatic transplants: new wrinkles.


Extracorporeal chemotherapy with L-asparaginase in man.


Everting ectopic ureteroceles.

Everting ectopic ureteroceles.


Symptomatic therapy in viral illness. A controlled study of effects on work performance.


Antenatal diagnosis and therapeutic trends in sphingolipidoses.


Minocycline (minocin).

Minocycline (minocin).


Explanation of degree of correlation of sibling generation times in animal cells.


Autosomal recessive mutation in sugar response of Drosophila.


Retardation of discrimination reversal by delta9-tetrahydrocannabinol in monkeys.


Electrolyte compatibility in a synthetic amino acid hyperalimentation solution.


Monitoring the effectiveness of antimicrobial agents in a general hospital.


Parenteral hyperalimentation--a quality of care survey and review.


Physiochemical considerations in intravenous hyperalimentation.


Stability of parenteral solutions of cefazolin sodium.


Hyaluronidase effects on aqueous outflow resistance. Quantitative and localizing studies in the rhesus monkey eye.


Primary malignant medulloepithelioma of the retinal stalk.


Experimental retinal detachment in the owl monkey. 10. The early receptor poential.


The effect of tobramycin on rabbit eyes.


The effect of tobramycin on gentamicin-resistant strains in Pseudomonas keratitis.


Cholinergic influence on intravenous cocaine self-administration by rhesus monkeys.


Acute tolerance to the effects of delta-9-tetrahydrocannabinol on spaced responding by monkeys.


The zoogeography of Polychromophilus and description of a new species of a gregarine (Lankesteria galliardi).


Letter: Prey-predator transmission in the epizootiology of ovine sarcosporidiosis.


Mechanics of ciliary locomotion.

Mechanics of ciliary locomotion.


Fibrous dysplastic lesions of the jaws in Nigerians.


Distribution of the Kell blood groups in the Spanish population.


Detection of relationships among microsporidan isolates by electrophoretic analysis: hydrophobic extracts.


The effect of cryosurgery on peripheral nerves.


The retinotectal projection from a double-ventral compound eye in Xenopus laevis.


Germ cell migration in toad (Bufo bufo): effect of ventral grafting of embryonic dorsal regions.


Detection of relationships among microsporidan isolates by electrophoretic analysis: hydrophilic extracts.


Intravenous hyperalimentation in cancer patients.


An immunologic rationale for suture line recurrence in carcinoma of the colon.


Family structure, child location and nutritional disease in rural Haiti.


Letter: Hyperosmolar diabetic coma--ketotic and nonketotic.


Study reveals increases--in costs, in per diem rates, and in per diem expenditures.


Associative learning of Drosophila melanogaster.


Negative reinforcing properties of morphine-antagonists in naive rhesus monkeys.


Association of pili with rough colony type of Moraxella bovis.


African swine fever: detection of antibody by reverse single radial immunodiffusion.


Scanning isoelectric focusing in small density-gradient colums. 3. A method for detailed mapping of biological activity of column contents applied to pea lipoxygenase.


Allergic reaction patterns in relation to age, sex, season, eosinophilia and some genetic marker systems.


Homocytotropic antibody in schistosomiasis mansoni.


Affinity chromatography of carbonic anhydrase.


The nose as a model for the study of respiratory tract allergic disease.


Fractionation and chemical characterization studies on Micropolyspora faeni antigens.


Immunology of byssinosis: a study of the reactions between the isolated byssinosis "antigen" and human immunoglobulins.


Root-end closure techniques including apexification.


Alpha crystallin subunits in the bovine lens.


Characterization of reaction centers from photosynthetic bacteria. II. Amino acid composition of the reaction center protein and its subunits in Rhodopseudomonas spheroides R-26.


Alpha-keto esters as substrates of erythrocyte carbonic anhydrase. Kinetic studies of enzyme-catalyzed hydration of methyl and ethyl pyruvate.


Thyrotropin response to thyrotropin releasing hormone in normal subjects.


Activity of neurons in the lower precentral cortex during voluntary and rhythmical jaw movements in the monkey.


Diclonal and multiple gammopathies.

Diclonal and multiple gammopathies.


Homologues of the human M-N blood types in gorillas and other non-human primates.


Glycolytic intermediates and adenine nucleotides of human platelets. I. The influence of ACD and EDTA anticoagulants.


Basilar membrane tuning curves in the guinea pig.


An evaluation of the latex agglutination test for the detection of hepatitis associated antigen.


Evidence from Mössbauer experiments for nonlinear vibration in the cochlea.


Study indicates which patients nurses don't like: the unpleasant, the long-term, the mentally ill, the hypochondriacs, and the dying.


Biologic monitor is best test of ETO sterilizer.


Membrane-to-membrane interactions in mitochondria treated with basic proteins.


Foci of degeneration produced by measles virus in cell cultures with antibody-free liquid medium.


The significance of post-traumatic status epilepticus in childhood. Five cases of early post-traumatic status epilepticus in childhood are reported. In all cases the trauma was apparently of a minor nature and the seizures appeared within two hours after injury. In none of the cases was a significant intracranial clot detected, nor was lasting cerebral damage evident. This is in contrast with adults, where status epilepticus after head injury is thought to be an ominous sign. Rapid improvement in level of consciousness after prompt treatment is certainly the most important criterion in assessing the role of the seizures. In the same period of time, post-traumatic status epilepticus was not seen in children with more severe head injury.


The relationship of a caprine mycoplasma to PG3, the type strain of Mycoplasma mycoides subsp. capri.


Incidence and significance of mycoplasmas in sick cats.


Influence and use of the employ of disodium cromoglycate "DSCG" on circadian rhythms in pulmonary mechanics.


Platelet aggregation by ristocetin in EDTA plasma: extension clumping with high concentrations of EDTA.


Reaginic antibodies and helminth infection.


Preprothrombin and prothrombin in full-term newborns.


Clinical symptoms induced by E. coli endotoxin in goats.


Hydroxyproline excretion in bovine puerperal paresis with special reference to bone resorption.


Intraluminal diverticulum of the duodenum--cass report and review of the literature.


Congenital hemihypertrophy with abnormalities of the aortic arch, and of the skeletal, cutaneous and ocular systems.


Whole body counting using a twin-detector scanner.


Preliminary observations on the influence of proflavin, ethidium bromide and elevated temperature on the production of the antibiotic nisin by Streptococcus lactis strains.


Changes occurring in proteins of bull semen plasma owing to inflammatory conditions of the efferent ducts.


A comparison of lorazepam, diazepam and placebo in the treatment of anxiety states.


Clinical and microbiological evaluation of pivampicillin in the treatment of urinary tract infections.


Nefopam hydrochloride for pain relief.

Nefopam hydrochloride for pain relief.


Treadmill exercise-tolerance test behavior in anginal patients treated with perhexiline maleate.


Effect of candicidin on fungal flora of the gastrointestinal tract.


Single-session, two-dose therapy with doxycycline for gonorrhea.


"Combination" anti-asthmatic therapy: recent developments.


Study of the serum half life of lividomycin in uraemic patients.


The possible role of the dog in the epidemiology of ovine sarcosporidiosis.


Doxycycline in renal insufficiency.

Doxycycline in renal insufficiency.


Comparative serum theophylline levels following the oral administration of elixophyllin and somophyllino-O, a new preparation of aminophylline.


A pharmacologic evaluation of fluoxymesterone in normal men.


Relapse in chronic urinary tract infection in a child due to Salmonella brandenburg.


The place of general practice.

The place of general practice.


Methods for the measurement of chloroplast volume and structure in vitro and in vivo.


Acid-base indicator for the measurement of rapid changes in hydrogen ion concentration.


Nitrogen fixation--assay methods and techniques.


Hydrogen ion buffers.

Hydrogen ion buffers.


Visual stimulus control of intracranial self-stimulation in the squirrel monkey (Saimiri sciureus).


Prefrontal lesions alter eating and hoarding behavior in rats.


The operational definition of human attachment.


Organic factors and psychophysiology in childhood schizophrenia.


Antibiotics produced by Streptomyces. IX. Heptafungin-A, a new heptaene macrolide antibiotic.


Effect of DDT and sevin on soil fungi.

Effect of DDT and sevin on soil fungi.


Regulated biosynthesis of protease and alpha-amylase in Bacillus subtilis.


Phenotypic derepression of enzymes of the isoleucine-valine pathway by isoleucine in Pseudomonas aeruginosa.


Proceedings: New outlook on the epidemiology and epizoology of listeriosis.


Serological relationship between Pseudomonas aeruginosa and Enterobacteriaceae. I. Relationship of Pseudomonas aeruginosa O antigens to Salmonella, Arizona and Citrobacter.


Proceedings: Listeria monocytogenes: some biochemical and serological aspects.


Proceedings: Listeriosis: its veterinary hygienic significance in Hungary.


Proceedings: Ability of Listeria monocytogenes strains to attack carbohydrates and related compounds.


Proceedings: Phosphatase activity of listeria.


Proceedings: Biological properties of virulent and avirulent Listeria monocytogenes strains.


Proceedings: Present situation of human listeriosis in Hungary.


Proceedings: Listeria monocytogenes-haemolysin: lecithinase.


Proceedings: Flagellation in virulent and avirulent Listeria monocytogenes strains.


Proceedings: Survival versus growth of a facultative psychrotroph.


Proceedings: Experience with nalidixic acid-trypaflavine agar.


Proceedings: Methods to isolate Listeria monocytogenes from different materials.


Proceedings: Differential media for the isolation of Listeria monocytogenes.


Proceedings: Listeriosis in professionally exposed persons.


Proceedings: Screening examination for listeriosis in pregnancy.


Proceedings: Serological screening of pregnant women for listeriosis.


Proceedings: Listeriosis in puerperal women and their infants.


Proceedings: Tetracycline treatment of pregnant women with suspected listeriosis.


Proceedings: Listeriosis and gestation.

Proceedings: Listeriosis and gestation.


Proceedings: Congenital listeriosis.

Proceedings: Congenital listeriosis.


Proceedings: Involvement of the central nervous system in neonatal listeriosis.


Proceedings: Listeric meningitis: report of a case.


Proceedings: The pathogenicity of Listeria monocytogenes.


Proceedings: Pathogenicity of Listeria monocytogenes in the rat.


Proceedings: An outbreak of listeriosis in domestic rabbits.


Proceedings: Active immunization with formol vaccine against sheep listeriosis.


Proceedings: Abortion in Gray's monkey (Cercopithecus mona) associated with Listeria monocytogenes.


The major dilemma of mental retardation: shall we prevent it? (Some social implications of research in mental retardation).


Immunoglobulins in aqueous humor and iris from endogenous uveitis and cataract patients.


Microsurgical studies. II. Corneal endothelial healing and posterior wound closure after "through and through" suturing.


Aphakic soft contact lenses. I. A clinical and bacteriological study.


Comparison of methods used for solubilization of human erythrocyte membrane protein.


The attachment of IgG molecules on the red cell surface.


Non-specific attachment of IgG to erythrocytes.


Methyldopa-induced erythrocyte binding IgG: another model of nonspecific attachment of immunoglobulins to erythrocytes.


Studies on the pyocins of Pseudomonas aeruginosa: morphology and mode of action of contractile pyocins.


Studies on the pyocins of Pseudomonas aeruginosa: production of contractile and flexuous pyocins in Pseudomonas aeruginosa.


The inhibition of growth of Escherichia coli spheroplasts by antibacterial agents.


Childhood lead poisoning: medical review.


The bioassay of rubratoxins A and B using Tetrahymena pyriformis strain W.


Effect of pH on Blepharisma intermedium. 2. Cytochemical changes.


Synthesis and antibacterial properties of substituted decylbarbituric acids.


2-Ethylpyridine derivatives with antitubercular properties.


Use of Medicaid payment files for medical care research.


Community hospitals and Medicaid.

Community hospitals and Medicaid.


Quality and cost control: Medicare and beyond.


New treatment for acanthosis nigricans.

New treatment for acanthosis nigricans.


Antimicrobial activity of sodium copper chlorophyllin.


Prevalence of Neisseria meningitidis serogroups in Egypt.


Results and appraisal of a nutrition survey in Malawi.


Amino acid ratios in protein-energy malnutrition.


Distribution, biotransformation and excretion of bupivacaine in the rat and the monkey.


Acute gonorrheal proctitis.

Acute gonorrheal proctitis.


Effect of oxytetracycline, doxycycline and folic acid on nitrogen balance during parenteral nutrition.


Unresponsiveness to thyrotropin-releasing hormone (TRH) in treated Graves' hyperthyroidism and in euthyroid Graves' disease.


15N studies of endogenous faecal nitrogen in infants. (15)N from labelled yeast protein, fed to marasmic and recovered infants, appeared in the stool shortly after administration and continued to be excreted for 100 hours. Unabsorbed dietary (15)N, which appeared with carmine-marked stools, formed only part of this excretion. Calculations of the endogenous nitrogen content of infant stool agreed closely with those of previous workers. The use of intravenously injected (15)N showed that part of the stool nitrogen was derived from this source. It was excreted as urea, ammonia, and other compounds, and totalled about 2% of the administered urea. The persistence of the (15)N in the stool suggests that it had in part been incorporated into cellular material.


Thyroxine-binding-globulin (T.B.G.) deficiency and glucose-6-phosphate dehydrogenase (G-6-PD) deficiency in the same family.


Comparison of serum prolactin and thyrotropin responses to thyrotropin-releasing hormone in patients with Graves' ophthalmopathy.


The inhibition of infection by cucumber mosaic virus and influenza virus by extracts from Phytolacca americana.


Physico-chemical evidence for the re-classification of the caliciviruses.


Serological studies with purified neuraminidase antigens of influenza B viruses. Neuraminidase (N) can be extracted from virus particles of influenza B strains by treatment with trypsin, in a form which is free from the viral HA and has specific immunological activity. The N antigen of B/LEE/40 behaves differently from that of 1965-6 strains in gel diffusion and enzyme inhibition tests with animal antisera raised by infection or by artificial immunization with the homologous or heterologous strains. The frequency and titres of NI antibody detected in human sera by B/LEE antigen are different from those found with antigen from B/Eng/13/65. The latter antibody appears to contribute to the effect of serum HI antibody in protecting volunteers exposed to a deliberate intranasal challenge infection of the B/Eng/13/65 strain.


Selective elimination of Enterobacteriaceae species from the digestive tract in mice and monkeys. In mice and in monkeys, ;selective' elimination of Enterobacteriaceae species from the digestive tract of animals with a sensitive flora was accomplished by oral treatment with nalidixic acid (1 mg./g. body weight in mice and 0.4 mg./g. body weight in monkeys). During treatment, the concentration of enterococci (and also of Candida albicans in the monkey) remained unaltered. This indicates that the fraction of the anaerobic microflora which is responsible for the colonization resistance of the digestive tract is not affected by the treatment. An important consequence seems to be, that elimination of yeast and fungi with fungistatic drugs can be started at the same time as elimination of Enterobacteriaceae is attempted.


Exposure to hepatitis B virus and development of the chronic HBAg carrier state in children.


Mechanism of depigmentation by hydroquinone.


Posthypoglycemic hyperglycemia in diabetic children.


Meningiomas of childhood and adolescence.


Fucosidosis: clinical, biochemical, immunologic, and genetic studies in two new cases.


The Winchester syndrome: a nonlysosomal connective tissue disease.


Therapeutic implications of electrolyte, water, and nitrogen losses during recovery from protein-calorie malnutrition.


Models of the saccadic eye movement control system.


Chemical nature of monogenean sclerites. I. Stabilization of clamp-protein by formation of dityrosine.


Immunochemical cross-reactivity between the dissociated capsid proteins of PVY group plant viruses.


Production of aflatoxin by cultures derived from conidia stored in the laboratory.


Are silicone rubber T-tubes better than latex rubber tubes in the common bile duct? A rhesus monkey model.


The effect of malnutrition on the pattern of growth in the rat kidney and the renal response to acidosis.


Radical total glossectomy.

Radical total glossectomy.


Simple and complex spike activities of the cerebellar Purkinje cell in relation to selective alternate movement in intact monkey.


Letter: Acute pancreatitis and diabetic ketoacidosis in hypothermia.


Studies on the control of some simple motor tasks. V. Changes in motor output following dorsal root section in squirrel monkey.


What constitutes adequate medical therapy for the patient with angina pectoris?


Letter: CCNU in teatment of recurrent medulloblastoma.


Clonal analysis of cell division in the Bacillus subtilis div IV-B1 minicell-producing mutant. Spores of the Bacillus subtilis minicell-producing mutant div IV-B1 were germinated and grown to microcolonies in chambers which facilitate continuous observation of the developing clones with a phase-contrast microscope. Time lapse photographs were taken of 46 clones, covering the period from the beginning of outgrowth until at least two rounds of cell division had been completed. Cell lineages were constructed from contour length measurements of the photographs. These data include cell lengths, division site locations, and cell numbers in clones of various ages. From these data we have determined that the probability of a minicell being produced at any division by the div IV-B1 mutant is 0.31. The location of the abnormal division site which generates the first minicell produced in the outgrowing clone appears to be random with respect to the existing cell poles. In contrast, the location of the second abnormal division site, and hence the second minicell, is not random but rather occurs preferentially in proximity to the first minicell. This clustering of abnormal events suggests that division site location is related to pole age (generations), although other influences on minicell clustering cannot be ruled out at present.


Transcriptional control in the L-arabinose operon of Escherichia coli B-r. The structural genes involved in l-arabinose metabolism are regulated by the protein product of the araC gene. This protein functions as both an activator and repressor of enzyme synthesis in this gene complex. Using lambdah80dara deoxyribonucleic acid in hybridization studies, we have shown that the ara operon, including structural genes araB, araA, and araD, is transcribed in the direction araB to araD and that initiation of transcription of these genes requires an active araC gene. The half-life of this message, approximately 3 min at 30 C, is the same in the presence or absence of the araC protein in the activator state. However, an unexplained 2-min lag in decay of ara messenger ribonucleic acid that does not occur in decay of lac messenger ribonucleic acid is observed. This lag period requires activated araC protein.


Direct enzymatic repair of deoxyribonucleic acid single-strand breaks in dormant spores. With the alkaline sucrose gradient centrifugation method, it was found that dormant spores of Clostridium botulinum subjected to 300 krads of gamma radiation showed a distinct decrease in deoxyribonucleic acid (DNA) fragment size, indicating induction of single-strand breaks (SSB). A two- to threefold difference in radiation resistance of spores of two strains of C. botulinum, 33A (37% survival dose [D(37)] = 110 krads) and 51B (D(37) = 47 krads), was accompanied by relatively larger DNA fragments (molecular weight 7.9 x 10(7)) obtained during extraction from the radiation-resistant strain 33A and smaller DNA fragments (molecular weight 1.8 x 10(7)) obtained under identical conditions from radiation-sensitive strain 51B. The apparent number of DNA SSB produced by 300 krads in strains 33A and 51B was 0.37 and 3.50, respectively, per 10(8) daltons of DNA. Addition of 0.02 M ethylenediaminetetraacetic acid (EDTA) to spore suspensions during irradiation doubled the apparent number of SSB in strain 33A but had no effect on strain 51B. In vivo, 0.02 M EDTA present during irradiation to 100 to 300 krads decreased survival of spores of 33A by about 30% but had little or no effect on 51B. Survival of 33A was also reduced by about 45% when the spores were irradiated while frozen in dry ice (-75 C) and, after irradiation, immediately exposed to 0.03 M EDTA for 1 h to inhibit repair in the dormant spores. These results suggest that the highly radiation-resistant strain 33A may be able to accomplish repair of SSB during irradiation or after irradiation under nonphysiological conditions, i.e., in the dormant state. This repair can be inhibited by EDTA. Sedimentation patterns show that DNA from spores of both strains 33A and 51B did not show any postirradiation repair during the first 6 h of germination, as opposed to Bacillus subtilis spores, which exhibit repair immediately after germination. These observations suggest the existence of direct repair in physiological dormant spores of strain 33A in the cryptobiotic resting state in the absence of germination. The repair seems to be similar to that of polynucleotide ligase activity shown to be operative in some vegetative cells. Apparently radiation-sensitive strains such as 51B and B. subtilis are generally poor in DNA repair enzyme activity under conditions of spore dormancy, which may account for the approximately threefold difference in radiation sensitivity or DNA fragility of different strains, or both.


Amino-terminal sequence of the tryptophan synthetase alpha chain of Bacillus subtilis. The sequence of the 46 NH(2)-terminal residues of the tryptophan synthetase alpha chain of Bacillus subtilis was determined and compared with the corresponding sequences of Escherichia coli, Shigella dysenteriae, Salmonella typhimurium, Aerobacter aerogenes, Serratia marcescens, and Pseudomonas putida. A deletion of six residues was found at the NH(2)-terminal end of the alpha chain of B. subtilis.


Use of ultraviolet radiation to locate dipicolinic acid in Bacillus cereus spores. A novel method is described that allows a direct determination of the location of a portion of the dipicolinic acid (DPA) in spores. Ultraviolet (UV) irradiation is shown to cause cross-linkage of DPA to spore proteins which have the characteristics of membrane proteins. We suggest that DPA resides in the inner forespore membrane (IFSM) and spore cytoplasm (i.e., the spore protoplast). Only that portion of the DPA in the vicinity of the IFSM appeared to form UV-induced DPA-protein adducts.


Initiation of protein synthesis by folate-sufficient and folate-deficient Streptococcus faecalis R: partial purification and properties of methionyl-transfer ribonucleic acid synthetase and methionyl-transfer ribonucleic acid formyltransferase. The initiation of protein synthesis by Streptococcus faecalis R grown in folate-free culture occurs without N-formylation or N-acylation of methionyl-tRNA(f) (Met). Methionyl-tRNA synthetase and methionyl-tRNA formyltransferase were partially purified from S. faecalis grown under normal culture conditions in the presence of folate (plus-folate); the general properties of the enzymes were determined and compared with the properties of the enzymes purified from wild-type cells grown in the absence of folate (minus-folate). S. faecalis methionyl-tRNA synthetase displays optimal activity at pH values between 7.2 and 7.8, requires Mg(2+), and has an apparent molecular weight of 106,000, as determined by gel filtration, and 127,000, as determined by sucrose density gradient centrifugation. The K(m) values of plus-folate methionyl-tRNA synthetase for each of the three substrates in the aminoacylation reaction (l-methionine, adenosine triphosphate, and tRNA) are nearly identical to the respective substrate Michaelis constants of minus-folate methionyl-tRNA synthetase. Furthermore, both plus- and minus-folate S. faecalis methionyl-tRNA synthetases catalyze, at equal rates, the aminoacylation of tRNA(f) (Met) and tRNA(m) (Met) isolated from either plus-folate or minus-folate cells. S. faecalis methionyl-tRNA formyltransferase displays optimal activity at pH values near 7.0, is stimulated by Mg(2+), and has an apparent molecular weight of approximately 29,900 when estimated by sucrose density gradient centrifugation. The K(m) value of plus-folate formyltransferase for plus-folate Met-tRNA(f) (Met) does not differ significantly from that of minus-folate formyltransferase for minus-folate Met-tRNA(f) (Met). Both enzymes can utilize either 10-formyltetrahydrofolate or 10-formyltetrahydropteroyltriglutamate as the formyl donor; the Michaelis constant for the monoglutamyl pteroyl coenzyme is slightly less than that of the triglutamyl pteroyl coenzyme for both transformylases. Tetrahydrofolate and uncharged tRNA(f) (Met) are competitive inhibitors of both plus- and minus-folate S. faecalis formyltransferase; folic acid, pteroic acid, aminopterin, and Met-tRNA(m) (Met) are not inhibitory. These results indicate that the presence or absence of folic acid in the culture medium of S. faecalis has no apparent effect on either methionyl-tRNA synthetase or methionyl-tRNA formyltransferase, the two enzymes directly involved in the formation of formylmethionyl-tRNA(f) (Met). Therefóre, the lack of N-formylation of Met-tRNA(f) (Met) in minus-folate S. faecalis is due to the absence of the formyl donor, a 10-formyl-tetrahydropteroyl derivative. Although the general properties of S. faecalis methionyl-tRNA synthetase are similar to those of other aminoacyl-tRNA synthetases, S. faecalis methionyl-tRNA formyltransferase differs from other previously described transformylases in certain kinetic parameters.


Phospholipid metabolism and membrane synthesis during sporulation in Bacillus megaterium. In view of previously published reports of localized membrane growth in exponentially growing Bacillus megaterium and in sporulating Bacillus cereus, an attempt was made to describe phospholipid metabolism and the topology of membrane synthesis during sporulation in B. megaterium. The cells were pulsed with radioactive glycerol or acetate at the time of septum formation, and the specific activity of the lipid fraction was measured at various times through the free spore stage. The bulk of the material labeled during septation could not be recovered in the spore. Rather, it was found that the labeled lipid fraction underwent considerable turnover during spore development. Additionally, other experiments revealed that the lipid made before the initiation of sporulation was also subject to extensive turnover. In order to minimize both the confounding effects of lipid turnover and the possible presence of lateral diffusion of labeled lipid in the membrane, autoradiography of cells pulse labeled with radioactive glycerol at the time of septation was performed; a symmetrical grain distribution resulted. Thus, despite previously published suggestions to the contrary, the current experimental techniques could not demonstrate the existence of localized membrane synthesis in B. megaterium during sporulation.


Glucose transport in Brucella abortus.

Glucose transport in Brucella abortus. Brucella abortus British strain 19 transported glucose with an apparent K(m) of 0.16 mM and an apparent V(max) of 250 nmol per min per mg of N. The only common glucose analogue transported was 2-deoxyglucose (2-DOG), with an apparent K(i) of 0.73 mM. Alpha- or beta-methyl glucosides and 3-O-methylglucose were not transported. Transport was linear for 70 to 90 s, depending on the concentration of substrate used. 2-Deoxyglucose was transported as the free sugar and was not further metabolized once inside the cell. There was no glucose phosphoenolpyruvate phosphotransferase system (PEP-PTS) present, and there were no inhibitors present in Brucella cell-free extract that inhibited the Escherichia coli glucose PEP-PTS. N-Ethylmaleimide (NEM) and p-chloromercuribenzoate (pCMB) completely inhibited transport of glucose and 2-DOG. Glutathione, dithiothreitol, and beta-mercaptoethanol reversed the effects of pCMB but not of NEM. A pH optimum of 7.2 and a temperature optimum of 37 to 45 C were observed for both K(m) and V(max). The glucose transport system appeared to be constitutive for glucose transport in cells grown on fructose, galactose, erythritol, or glucose. The electron transfer inhibitors carbonyl cyanide, m-chlorophenylhydrazone, NaN(3), 2,4-dinitrophenol, and KCN inhibited 2-DOG transport to a greater extent than did the metabolic energy inhibitors NaAsO(4), iodoacetate, KF, and 2-heptyl-4-hydroxyquinoline-N-oxide. Dicyclohexylcarbodiimide, an inhibitor of membrane-bound adenosine triphosphatases, inhibited transport by 100%.


Exclusion of induced bacteriophage from cells of a lysogenic Bacillus megaterium committed to sporulation. Spontaneous release of the temperate bacteriophage T (phiT), carried by Bacillus megaterium 899a, occurred during early growth of the host cells. Rejuvenated cells (accomplished by a 5x dilution in fresh medium) and unrejuvenated cells were induced by mitomycin C during the course of sporulation and subsequent phage phiT production measured by burst size. Induction of sequential samples of unrejuvenated cells resulted in burst sizes that fell to zero as T(0) sporulation time in the main culture was approached. This drop in burst size was not considered a sporulation event, as it also occurred during analogous stages of growth in an asporogenous mutant. Rejuvenated, induced portions of the culture of sporulating cells of B. megaterium 899a gave large burst sizes until T(+2), when the burst sizes fell to zero. The stage I asporogenous mutant, treated in a similar manner, gave lower, but still substantial, burst sizes; thus, the sharp decline in burst size of induced rejuvenated sporulating cells appeared to be a sporulation event. Sporulating cells induced at times shortly after T(1.5) formed spores in which the induced phage were trapped until germination of the spores, which formed infectious centers. This induced phage-trapping was maximal when the sporulating cultures were induced at T(2.25). Commitment to sporulation could be defined by our system as that point beyond which rejuvenated sporulating cells were unable to support the replication of the phage. This point also correlated with the increase in induced phage-trapping by spores. Two other methods gave a similar commitment time. Commitment to sporulation, in spite of added glucose or fresh complex medium, occurred at the same time. Electron micrography showed that the committed cell was still undergoing engulfment. The fate of induced phage phiT was determined at different points during growth and sporulation. Induction at times prior to T(0), which were longer than the eclipse period of the phage, resulted in a burst size of approximately 50. At times prior to T(0), shorter than the phage eclipse period, induction led to lysis with low burst sizes, approaching zero. The pattern of spontaneous phage release during growth was similar. From T(0) up to the point of commitment to sporulation, induction resulted in the blocking of spore formation without lysis. At the commitment point, induced phage were trapped and carried into spores which germinated to give infectious centers. The spontaneous derepression of phage at a time which blocked spore formation led to 7 x 10(4) infectious centers per ml and would not normally be noticed. Derepression at the time of phage entrapment was not observed to occur without induction with mitomycin C.


Subunit cell wall of Sulfolobus acidocaldarius. The cell wall of Sulfolobus acidocaldarius has been isolated. Cells were mechanically disrupted with a French press, and the cytoplasmic membrane was removed by extracting cell-envelope fragments with Triton X-100. The Triton-insoluble cell wall material retained the characteristic subunit structure when examined in the electron microscope. Isolated cell wall fragments formed in open sheets that were easily separated from cytoplasmic contamination. Chemical studies showed that the Triton-insoluble cell wall fragments consisted of lipoprotein with small amounts of carbohydrate and hexosamine. The amino acid composition indicated a highly charged hydrophobic cell surface. The presence of diaminopimelic acid with only traces of muramic acid indicates that the cell envelope does not have a rigid peptidoglycan layer. The results of chemical analyses and electron microscopy suggest a wall-membrane interaction stabilizing the cell envelope. The chemical and physical properties of this type of cell envelope would appear to form the basis for a new major division of bacteria with the definitive characteristics of a morphologically distinct subunit cell wall devoid of peptidoglycan.


Properties and developmental roles of the lysyl- and tryptophanyl-transfer ribonucleic acid synthetases of Bacillus subtilis: common genetic origin of the corresponding spore and vegetative enzymes. The lysyl-transfer ribonucleic acid synthetase (LRS) and tryptophanyl-transfer ribonucleic acid synthetases (TRS) (l-lysine:tRNA ligase [AMP], EC 6.1.1.6; and l-tryptophan:tRNA ligase [AMP], EC 6.1.1.2) have been purified 60- and 100-fold, respectively, from vegetative cells and spores of Bacillus subtilis. There are no significant differences between the corresponding spore and vegetative enzymes with respect to their elution characteristics from columns of phosphocellulose or hydroxylapatite, their molecular weight (~130,000 for LRS and ~87,000 for TRS as determined by gel filtration), their kinetic constants for substrates (in the amino acid-dependent adenosine triphosphate-pyrophosphate exchange reaction), and the kinetics of inactivation by heat and by antibody. The Mg(2+) requirement for optimal enzyme activity of the corresponding spore and vegetative enzyme differ slightly. Mutants having defective (temperature sensitive) vegetative LRS or TRS activities produce spores in which these enzymes are also defective. The mutant spores are more heat sensitive than the parental type, but contain normal levels of dipicolinic acid. They germinate normally at the restrictive temperature (43 C), but are blocked at specific developmental stages in outgrowth. No modification in temperature sensitivity phenotype occurs during outgrowth, nor is there a change in molecular weight of the two enzymes. The implication is that the LRS and TRS activities of the vegetative and spore stages are each coded (at least in part) by the same structural gene. The temperature sensitivity of mutant spores is discussed with respect to those factors which are involved in the formation of the heat-resistant state.


Alloantibodies and Australia antigen after open heart surgery. The development of irregular serum antibodies (alloantibodies) following massive transfusion was studied in 144 patients who had undergone cardiac valve replacement. An overall incidence of alloimmunization of 8.3% was found. Of the 15 antibodies detected nine were Rhesus anti-E and four anti-Kell. The incidence of anti-E formation in Rhesus E-negative patients was 11.4%; the corresponding figure for anti-Kell was 3.2%. The results suggest that the risk of allo-immunization is directly related to the volume of blood transfused, and Rhesus E-negative persons appear to be at particular risk. Australia (Au) antigen investigations were also carried out in 102 of these cases. Three patients were Au antigen positive, one of them developing acute hepatitis. In each case no Au antigen could be detected in the donor blood that was used. It is suggested that tests for alloantibodies and for the Australia antigen should become part of the routine follow-up of any patient receiving massive transfusion.


Host-parasite relationship in echinococcosis. I. Parasite biomass and antibody response in three strains of inbred mice against graded doses of Echinococcus multilocularis cysts.


Leucocytozoon grusi sp. n. (Sporozoa: Leucocytozoidae) from a sandhill crane, Grus canadensis (L.).


Observations on the relapse activity of Plasmodium simiovale in the rhesus monkey.


Immunofluorescent staining of plasmodial oocysts in the mosquito.


Experimental infection of monkeys with Nanophyetus salmincola.


Parasites of golden trout from California.


Effect of immune rabbit IgG on the primary immune response to Trichinella spiralis in mice.


Embryonic neuropathy. A hypothesis of neural chest injury as the pathogenesis of congenital malformations.


Rh(D) immunization after massive transfusion of Rh(D)-positive blood.


Red blood cell velocity in nailfold capillaries of man measured by a television microscopy technique.


Inhibition of Pseudomonas aeruginosa by ascorbic acid acting singly and in combination with antimicrobials: in-vitro and in-vivo studies.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 19-1974.


Schwann cell and myelin changes caused by some toxic agents and trauma.


Temporomandibular arthropathy.

Temporomandibular arthropathy.


Letter: Mycetoma.

Letter: Mycetoma.


South American trypanosomiasis (Chagas' disease).


The effects of early malnutrition in man on body composition and adipose tissue cellularity at adult age.


A clinical syndrome with inborn defect in erythropoiesis, dysplastic kidneys, eye lesions, malformation of the teeth and impaired hearing. A new syndrome in a 28-year-old woman.


Evaluation of primate allografts following treatment with immuosuppression and ameroid constrictors.


A technique for the prevention of pneumothorax in pulmonary aspiration biopsy.


Evidence for a retinohypothalamic pathway in the golden hamster.


IgG, IgA, IgM, and IgD antiglobulins in juvenile rheumatoid arthritis.


Identification of bacteria from blood and joint fluids of human subjects as Bacillus licheniformis.


Articular and cutaneous manifestations of gonorrhoea. Review of sixteen cases.


A simple graphical method for determining the inhibition constants of mixed, uncompetitive and non-competitive inhibitors. A new method is described for plotting kinetic results for inhibited enzyme-catalysed reactions. It provides a simple way of determining the inhibition constant, K'(i), of an uncompetitive, mixed or non-competitive inhibitor.


Preparation of some immobilized linked enzyme systems and their use in the automated determination of disaccharides. 1. Glucose oxidase (EC 1.1.3.4), amyloglucosidase (EC 3.2.1.3), invertase (EC 3.2.1.26) and beta-galactosidase (EC 3.2.1.23) were covalently attached via glutaraldehyde to the inside surface of nylon tube. 2. The linked enzyme system, comprising invertase immobilized within a nylon tube acting in series with glucose oxidase immobilized in a similar way, was used for the automated determination of sucrose. 3. The linked enzyme system, comprising beta-galactosidase immobilized within a nylon tube acting in series with glucose oxidase immobilized in a similar way, was used for the automated determination of lactose. 4. The linked enzyme system, comprising amyloglucosidase immobilized within a nylon tube acting in series with glucose oxidase immobilized in a similar way, was used for the automated determination of maltose. 5. Mixtures of glucose oxidase and amyloglucosidase were immobilized within the same piece of nylon tube and used for the automated determination of maltose. 6. Mixtures of glucose oxidase and invertase were immobilized within the same piece of nylon tube and used for the automated determination of sucrose.


Inhibition of enzymes by metal ion-chelating reagents. Theory and new graphical methods of study. 1. The mechanism of inhibition of enzymes by metal ion-chelating reagents is discussed and equations derived. 2. Two distinct mechanisms are postulated and graphical methods are given for differentiating between them. 3. Where the metal ion is actually removed from the enzyme to form a co-ordination complex in solution, a procedure is described for obtaining the stability constant for metal-enzyme interaction, the number of metal ions involved and the stoicheiometry of metal ion-ligand interaction.


Distribution of sulphate and iduronic acid residues in heparin and heparan sulphate. 1. A method was developed for determination of the uronic acid composition of heparin-like glycosaminoglycans. Polymers or oligosaccharides are degraded to monosaccharides by a combination of acid hydrolysis and deamination with HNO(2). The resulting uronic acid monosaccharides (accounting for about 70% of the uronic acid contents of the starting materials) are isolated and converted into the corresponding aldono-1,4-lactones, which are separated by g.l.c. The calculated ratios of glucuronic acid/iduronic acid are reproducible within 5%. 2. Samples of heparin from pig intestinal mucosa (molar ratio of sulphate/disaccharide unit, 2.40) and heparan sulphate from human aorta (sulphate/disaccharide ratio, 0.46) were subjected to uronic acid analysis. l-Iduronic acid constituted 77% and 19% respectively of the total uronic acid contents. 3. The correlation between the contents of sulphate and iduronic acid indicated by this finding also applied to the fractionated deamination products of the two polymers. The sulphated fragments varied in size from disaccharide to octasaccharide (or larger) and showed sulphate/disaccharide molar ratios in the range of 0.05-2.0. The proportion of iduronic acid increased with increasing ester sulphate contents of the oligosaccharides. 4. Previous studies on the biosynthesis of heparin in a cell-free system have shown that l-iduronic acid residues are formed by C-5 epimerization of d-glucuronic acid units at the polymer level; the process requires concomitant sulphation of the polymer. The results obtained in the present structural study conform to these findings, and suggest further that similar mechanisms may operate in the biosynthesis of heparan sulphate. The epimerization reaction appears to be linked to the sulphation of hydroxyl groups but does not seem to require sulphation of the target uronic acid residues. The significance of sulphamino groups in relation to the formation of iduronic acid is unknown.