The effect of a chemically defined diet on the faecal flora and faecal steroid concentration. The consumption of a chemically defined diet altered both the faecal flora and faecal steroids. Enterobacteria increased while enterococci and other lactic acid bacteria decreased in number. No other obvious change occurred. However, the alteration in the degree of degradation of the faecal neutral steroids may reflect some alteration in the anaerobic flora. Total excretion of both acid and neutral steroids was also reduced. On cessation of the diet both faecal flora and faecal steroids reverted to levels before the diet.


Postoperative intraperitoneal adhesions: a double-blind assessment of their prevention in the monkey. The effect of combined antihistamine and corticosteroid therapy and the effect of dextran on recurrent intraperitoneal adhesion formation were evaluated in 43 monkeys, all of which had been previously used for reproductive tract studies, and, hence, were suffering from adhesion formation to some degree.  In a double-blind manner, the monkeys were randomly divided into 3 experimental groups.  1 group received saline injections before and after lysis surgery followed by introduction of saline solution in the peritoneum during surgery.  Group 2 was treated with saline injections, as Group 1, except that dextran solution was introduced into the peritoneal cavity.  In Group 3, a combined dexamethasone/promethazine therapy was administered before, during, and after surgery.  In all of these monkeys, the degree of adhesion reformation after adhesion lysis surgery was essentially the same in all 3 groups.  Rather, careful surgical technique was vastly more important than use of corticosteroids or antihistamines as prophylactic measures.


Proceedings: The distribution of vasoactive intestinal peptide (VIP) in the primate gastrointestinal tract and characterization of VIP from human tumours.


Proceedings: Comparative effect of intravenous and oral nutrition of intestinal adaptation.


Avulsion of lumbar roots complicating fracture of the pelvis.


Pseudomonas vertebral osteomyelitis in heroin addicts. Report of five cases.


Gold distribution in whole blood during chrysotherapy.


Beta-glucuronidase deficiency mucopolysaccharidosis: methods for enzymatic diagnosis.


Neuritic (senile) plaques and filamentous changes in aged rhesus monkeys.


Mental disorders associated with industrial head injury.


Editorial: Gonococcocidal action of copper.


Editorial: Gallstones and estrogens.

Editorial: Gallstones and estrogens.


Letter: Diagnosis of Fabry's disease by tear alpha-galactosidase A.


A fate map of larval organs of Drosophila and preblastoderm determination.


Thymosin-producing cells of the thymus.

Thymosin-producing cells of the thymus.


The effect of capping H-2 antigens on the susceptibility of target cells to humoral and T cell-mediated lysis.


Regulation of rat liver beta-hydroxy-beta-methylglutaryl-CoA reductase activity by cholesterol.


Hypothalamic interaction between prostaglandins and catecholamines in promoting gonadotrophin secretion for ovulation.


Lead poisoning in childhood: a case report with environmental implications.


Immunoglobulins in normal porcine tracheobronchial secretions.


Kinetics of multinucleate giant cell formation and their modification by various agents in foreign body reactions. Foreign body multinucleate giant cells were produced by the implantation of a strip of Melenex in the subcutaneous tissues of mice. The implants were removed at various intervals, and the proportion of multinucleate giant cells as well as the number of nuclei they contained were counted and statistically assessed. The greatest proportion of giant cells was reached 4 weeks after implantation, when 25% of the attached cells were multinucleated. The mean nuclear content however was greatest approximately 2 weeks after implantation and rapidly fell over the ensuing weeks. The fusion potential however, remained almost unaltered for the remainder of the experimental period. Transplantation of 7-day Melenex implants from normal donors into lethally irradiated recipients demonstrated that the halflife of the giant cells is only a few days. Treatment with carrageenin, species-specific antisera, actinomycin D and cortisone inhibited, while puromycin enhanced, multinucleate cell formation. Calcium gluconate, EDTA and irradiation had no significant effect. The possible interpretation of some of these findings is discussed.


Pathology of Bolivian hemorrhagic fever in the rhesus monkey. Gross and microscopic lesions associated with Bolivan hemorrhagic fever virus infection in the rhesus monkey were studied in 10 animals which died following inoculation. Gross lesions included skin rash, lymphadenopathy, splenomegaly, meningeal edema, hydropericardium and enlarged friable livers. Hemorrhagic manifestations of the infection were not consistently observed, but hemorrhages were present in the skin, heart, brain and nares in some monkeys. Histopathologic lesions were fairly consistent. Hepatic necrosis with the presence of acidophilic hyaline bodies, necrotizing enteritis, epithelial necrosis and adrenal cortical necrosis were present in all monkeys. Those monkeys which died after the seventeenth day of infection had nonsupurative meningoencephalitis; lymphoid necrosis was present in 3 monkeys that died after day 18. Other microscopic lesions included myocardial degeneration, lymphoid and reticuloendothelial cell hyperplasia and lymphoid depletion. Most of the histopathologic lesions described in human autopsy material were reproduced; however, the necrosis in the skin and oral mucosa, mucosa of the gastrointestinal tract and the adrenal cortex have not been described in man. Despite these apparent discrepancies the results of this investigation indicate that the rhesus monkey is a good experimental model for the study of Bolivian hemorrhagic fever infection.


Mechanism of hyperalimentation in the suppression of upper gastrointestinal secretions.


Congenital duodenal atresia.

Congenital duodenal atresia.


Screening and isolation of penicillinase inhibitor, KA-107. It is known that penicillin resistance of bacteria is mainly caused by the inactivation of penicillin by penicillinase derived from such strains. We have developed a screening procedure for penicillinase inhibitors. Several microorganisms were found to produce such inhibitors, and from the culture filtrate of Streptomyces gedanensis ATCC 4880 a penicillinase inhibitor, named KA-107, was isolated. The characteristics of this inhibitor were revealed by an in vitro test by using penicillinase derived from penicillin resistant Staphylococcus aureus, FS-1277. When KA-107 was used in combination with penicillin-G, ampicillin, d- or l-phenethicillin, the growth inhibitory activity of these penicillins was maintained.


Protein synthesis in Mycobacterium tuberculosis H37Rv and the effect of streptomycin in streptomycin-susceptible and -resistant strains. An efficient in vitro amino acid-incorporating system from Mycobacterium tuberculosis H37Rv was standardized. Ribonucleic acid (RNA) isolated from phage-infected M. smegmatis cells served as natural messenger RNA and directed the incorporation of (14)C-amino acids into protein. The effects of various antitubercular drugs and "known inhibitors" of protein synthesis on amino acid incorporation were studied. Antibiotics like chloramphenicol and tetracycline inhibited mycobacterial protein synthesis, though they failed to prevent the growth of the organism. This failure was shown to be due to the impermeability of mycobacteria to these drugs by use of "membrane-active" agents along with the antibiotics in growth inhibition studies. Several independent streptomycin-resistant mutants of M. tuberculosis H37Rv were isolated. Streptomycin inhibited the incorporation of (14)C-amino acids into proteins by whole cells of a streptomycin-susceptible strain by more than 90%, whereas very little or no inhibition was observed in either high-level or low-level streptomycin-resistant strains. In vitro, streptomycin was an effective inhibitor of susceptible strains, whereas in streptomycin-resistant strains the concentration of streptomycin at which half-maximal inhibition was produced varied according to the resistance of whole cells, and there was a correlation between the two. In one low-level streptomycin-resistant mutant, the in vitro amino acid-incorporating system was as sensitive to various concentrations of streptomycin as the parental type, and a possible involvement of a membrane site in the development of low-level resistance was indicated. Streptomycin susceptibility and high-level resistance were shown to be ribosomal in nature.


Additional antibiotic inhibitors of peptidoglycan synthesis. Diumycin, janiemycin, nisin, and subtilin inhibited peptidoglycan synthesis catalyzed by particulate enzyme systems from Bacillus stearothermophilus and Escherichia coli. All of these, except for nisin, also induced accumulation of the lipid intermediate in peptidoglycan synthesis. Concentrations required for 50% inhibition of peptidoglycan synthesis were less than 0.1 mug/ml for diumycin and in the range of 10 to 100 mug/ml for janiemycin, nisin, and subtilin in both organisms. The discrepancy between the extremely low concentration of diumycin required to inhibit the in vitro system from E. coli and the much higher concentration required to inhibit growth of the organism is noteworthy.


Studies on the susceptibility of 150 consecutive clinical isolates of Pseudomonas aeruginosa to tobramycin, gentamicin, colistin, carbenicillin, and five other antimicrobials. The in vitro susceptibilities of 150 clinical isolates of Pseudomonas aeruginosa to the aminoglycoside tobramycin and eight other antimicrobials were evaluated. The Food and Drug Administration standardized disk diffusion method showed that tobramycin inhibited 100% of the 150 isolates with gentamicin inhibiting only 90.7%. The difference between colistin (97.3%) and tobramycin was less marked. Carbenicillin (87.3%) was found to be slightly less active than gentamicin. Only a small percentage of the isolates were inhibited by the other drugs used. By using a minimal inhibitory concentration of 4 mug/ml in brain heart infusion broth as the level of susceptibility, it was found that only three of the isolates showed resistance to tobramycin, whereas 20 of the isolates were resistant to gentamicin. Approximately half of the isolates were inhibited by 0.5 mug/ml or less of tobramycin, whereas 1.5 mug of gentamicin per ml was required to inhibit 50% of susceptible strains.


Lipid composition of gram-negative bacteria, sensitive and resistant to streptomycin. Analyses of streptomycin-sensitive and -resistant gram-negative bacteria show that, contrary to previous reports, the development of antibiotic resistance is not accompanied by changes in membrane lipid or fatty acid composition.


In vitro activity of cephalothin and three penicillins against Escherichia coli and Proteus species. The susceptibility of clinical isolates of Escherichia coli (67) and Proteus species (58) to cephalothin, ampicillin, benzyl penicillin, and phenoxymethyl penicillin was determined in vitro by using broth dilution and disk diffusion tests. The data were correlated by using a four-category scheme for interpreting minimal inhibitory concentrations (groups 1 to 4) as recommended by a subcommittee of an international collaborative study of susceptibility testing. With cephalothin and ampicillin, groups 1 (susceptible) and 2 (moderately susceptible) were susceptible by the disk test, and with benzyl penicillin, similar results were observed when the interpretive zone standards were changed. Strains categorized as group 4 (very resistant) were resistant by the disk method, but group 3 (moderately resistant) strains were not adequately distinguished by disk testing. Group 3 susceptibility to benzyl and phenoxymethyl penicillins can be predicted by extrapolating results from tests with ampicillin disks.


Rearing infant rhesus together.

Rearing infant rhesus together.


International symposium on gonorrhea.

International symposium on gonorrhea.


The in vitro inhibition of growth of intracellular Listeria monocytogenes by lymphocyte products.


The occurrence of 2-hydroxybutyric acid in urine from patients with lactic acidosis.


Serum alpha 2-macroglobulin levels in normal Chinese.


Generation of chemotactic activity by immune complexes carrying clustered or nonclustered C42 sites.


Immunoglobulins in the intestinal content of the human fetus with special reference to IgA.


Isolation of F(c)5mu and Fabmu fragments of human IgM.


Effects of nalidixic acid, chloramphenicol, cycloheximide, and anisomycin on structure and development of plastids and mitochondria in greening Euglena gracilis.


Immunochemical quantitation of enzymes in human diploid cell line WI-38.


3H-colcicine binding. Failure to detect any binding to soluble proteins from various lower organisms.


A cycloheximide-resistant mutant of Tetrahymena pyriformis.


Antibody-mediated internalization of B lymphocyte surface membrane immunoglobulin.


Effects of epididymal maturation, zinc (II) and copper (II) on the reactive sulfhydryl content of structural elements in rat spermatozoa.


Effect of inhibitors of RNA and protein synthesis on the aldosterone Na+ transport response in toad bladder.


The peritoneal fluid cytology of the adult female Rhesus monkey.


Aflatoxin production in some varieties of soybeans (Glycine max L.).


Studies on carrageenan and large-bowel ulceration in mammals.


Intestinal effects of carrageenans in the rhesus monkey (Macaca mulatta).


Excretion and metabolism of orally administered aflatoxin B1 by rhesus monkeys.


Panendoscopy in the early diagnosis of acute upper gastrointestinal bleeding.


Total parenteral nutrition at home for 23 months, without complication, and with good rehabilitation. A study of technical and metabolic features.


Further evidence of the inhabitation in a marginal population.


Chromatin fibril size in salivary gland chromosomes of Drosophila melanogaster.


Core fibrils in early mitotic prophase chromosomes of Drosophila melanogaster.


Mating behaviour and chaete number in Drosophila melanogaster.


Polymorphism at two esterase loci in Drosophila melanogaster from northern Greece.


Response of puffing pattern to in vivo treatments with organomercurials in Drosophila melanogaster.


Degradation of immunoglobulins by lysosomal enzymes of tumors. I. Demonstration of the phenomenon using mouse tumors.


Heat-polymerization of human immunoglobulin G.


Heterogeneity of the carcinoembryonic antigen.


Different susceptibility of subclasses of rat IgG2 to tryptic digestion.


Phototherapy in Rh incompatibility hyperbilirubinaemia.


Nutritional status of refugees from Bangla Desh.


Quantitative model for the effects of stimulus frequency upon synchronization of auditory nerve discharges.


Letter: Precedence effects and auditory cells with long characteristic delays.


Letter: Total synthesis and pharmacological activities of N-substituted 3,14-dihydroxymorphinans.


Experience with glibenclamide.

Experience with glibenclamide.


Behavioral regulation of gravity: schedule effects under escape-avoidance procedures. Squirrel monkeys were restrained in a centrifuge capsule and trained to escape and avoid increases in artificial gravity. During escape-avoidance, lever responses reduced centrifugally simulated gravity or postponed scheduled increases. The effect of variation in the interval of postponement (equal to the duration of decrease produced by escape responses) was studied under a multiple schedule of four components. Three components were gravity escape-avoidance with postponement times of 20, 40, and 60 sec. The fourth component was extinction. Each component was associated with a different auditory stimulus. Rate of responding decreased with increasing postponement time and higher mean g-levels occurred at shorter intervals of postponement. Effects of the schedule parameter on response rate and mean g-level were similar to effects of the schedule on free-operant avoidance and on titration behavior maintained by shock.


New drugs and research: glybenclamide (Euglucon).


Appropriate placement of the chronically ill and aged. A successful approach by evaluation.


Present knowledge of the relationship of nutrition to brain development and behavior.


Autoimmune hemolytic anemia due to levodopa therapy.


Open and closed treatment of burns with povidone-iodine.


Snythesis of analogs of the thyrotropin releasing hormone (TRH) and bioassays for TRH- and TRH-inhibiting activities.


Congenital cardiac diverticulum.

Congenital cardiac diverticulum.


Pathologic changes in baboon lung allografts. Comparison of two immunosuppression regimes.


Effects of gram negative endotoxemia on myocardial contractility in the awake primate.


Intraluminal diverticulum of the duodenum.


The threshold and neuropathology of cerebral "anoxic-ischemic" cell change.


Reversibility of ischemic brain damage.

Reversibility of ischemic brain damage.


Two classes of dysergic brain abnormality and their conditions of occurrence.


Antigenic properties of rat brain proteins fraction.


Experimental investigations on interactions between gentamicin and betamethasone dipropionate.


Congenital membranous stenosis of the midesophagus. A case report and literature survey.


A specific increase in cardiovascular reactivity related to sodium retention in DOCA-salt-treated rats.


The detection and localization of carbonic anhydrase in the rabbit cornea.


Letter: Fractionation of the soluble proteins of chick lens on Sephadex column.


Nucleus of the accessory optic tract. Light and electron microscopic study in normal monkeys and after eye enucleation.


Loss of visual control of the forelimb after interruption of cortical pathways.


Injury-induced long-first-interval bursts in cortical neurons.


Antiglobulins and cryoglobulins in rabbits producing homogeneous streptococcal antibodies.


Experimental cryoglobulinaemia. Production and properties of streptococcus-induced rabbit cryoglobulins.


The serological response of the chicken to a protein antigen in multiple emulsion oil adjuvant.


Modification of the enzymic activity of trypsin by intramolecular cross-links.


Giant cell arteritis--the need for prolonged treatment.


Proceedings: Asynchronous pulsatile luteinizing hormone and follicle-stimulating hormone responses during luteinizing hormone-follicle-stimulating hormone releasing hormone and thyroid-stimulating hormone releasing hormone infusions.


Proceedings: Distribution of radioimmunoassayable gastrin, secretin, pancreozymin and enteroglucagon in rat, dog, and baboon gut.


Proceedings: Prolactin release in the ewe induced by a synthetic tripeptide.


Effects of psychoactive drugs on nonverbal communication and group social behavior of monkeys.


Activity of magnocellular neuroendocrine cells in the hypothalamus of unanaesthetized monkeys. II. Osmosensitivity of functional cell types in the supraoptic nucleus and the internuclear zone. 1. We studied magnocellular neuroendocrine cells and non-neuroendocrine cells in the supraoptic nucleus (n.s.o.) and internuclear zone (i.n.z.) in the hypothalamus of unanaesthetized, chronically prepared monkeys. After antidromic identification, functional cell typing and sensory testing we injected solutions of varying tonicity into an implanted carotid cannula to determine osmosensitivity.2. On the basis of the anatomical location of the cells, the pattern of discharge in response to osmotic stimuli, the effect of posterior pituitary gland stimulation and the response to non-noxious arousing sensory stimuli, we divided the 101 osmosensitive cells studied into two major groups: eighty-nine (88%) ;specific' biphasic osmosensitive magnocellular neuroendocrine cells and twelve (12%) ;non-specific' monophasic osmosensitive non-neuroendocrine cells.3. ;Non-specific' non-neuroendocrine osmosensitive cells included nine (9%) cells lying in the internuclear zone (i.n.z.) and showing monophasic exictatory or inhibitory responses to both osmotic and to mildly arousing sensory stimuli. Three (3%) ;high-frequency burster' (h.f.b.) osmosensitive cells were located in the n.s.o.-t.o. (supraoptic nucleus-optic tract) junction, showed accelerated discharge to auditory, but not to light or touch stimuli, and were monophasically inhibited by osmotic stimuli.4. Thirty-three (33%) antidromically ;identified' magnocellular neuroendocrine cells in the n.s.o. and i.n.z. we classify as ;specific' biphasic osmosensitive cells on the basis of the excitatory-inhibitory response to intracarotid hypertonic sodium chloride and the lack of response to non-noxious arousing sensory stimuli. Fifty-six (55%) of the ;non-identified' magnocellular neuroendocrine cells in the n.s.o. and i.n.z. we also found to be ;specific' biphasic osmosensitive cells.5. Magnocellular neuroendocrine cells in n.s.o. and i.n.z. exhibited three functional types, ;silent' (s.), ;continuously active' (c.a.), and ;low frequency burster' (l.f.b.), all of which were osmosensitive with a ;specific' biphasic response. With osmotic loading some of the ;s.' cells tended to shift transiently to ;c.a.' firing, an occasional ;c.a.' cell burst briefly and a few ;l.f.b.' cells increased bursting for a short time (60 sec).6. We conclude that magnocellular neuroendocrine cells in n.s.o. and i.n.z. respond ;specifically' to intracarotid hypertonic sodium chloride with biphasic, excitatory-inhibitory, discharges and do not respond to mildly arousing sensory stimuli. We suggest that, under the conditions of our experiments, the three functional types of magnocellular neuroendocrine cells (s., c.a., l.f.b.), arising from a common primitive neuroendocrine cell, are randomly dispersed in n.s.o. and i.n.z., are each ;specialized' for secretion of a ;specific' neurohypophysial hormone, and are sensitive to ;specific' input connexions. Under this ;specific hormonal state' hypothesis we explain the paradoxical and uniform osmosensitivity of these three different functional cell types on the basis of a suprathreshold osmotic stimulus and a residual ;primitive' osmosensitivity. At present we are unable to say which functional magnocellular neuroendocrine cell types secrete a particular neurohypophysial hormone.


Intravascular coagulation--a source of possible error in carbon-clearance determinations.


Activity of magnocellular neuroendocrine cells in the hypothalamus of unanaesthetized monkeys. I. Functional cell types and their anatomical distribution in the supraoptic nucleus and the internuclear zone. 1. We recorded with tungsten micro-electrodes the spontaneous and evoked activity of single cells in the supraoptic nucleus (n.s.o.) and internuclear zone (i.n.z.) of trained, unanaesthetized monkeys who accepted experimental restraints and pituitary gland stimulation without anxiety.2. Of the 125 hypothalamic neurones analysed, 109 (87%) we classified as magnocellular neuroendocrine cells on the basis of the anatomical location of the cells, the pattern of spontaneous discharge, the effect of pituitary gland stimulation and the response to arousing sensory stimuli.3. Sixteen (13%) of the cells studied under identical conditions we labelled as non-neuroendocrine cells. Located in the i.n.z. and supraoptic nucleus-optic tract junction (n.s.o.-t.o.), these cells responded to arousing sensory stimuli, showed ;continuously active' (i.n.z.) and ;high-frequency burster' (n.s.o.-t.o.) patterns of discharge and were not driven by pituitary stimuli.4. Thirty-seven (30%) of the magnocellular neuroendocrine cells we describe as ;identified' because with pituitary gland stimulation we evoked antidromic potentials, mean latency 8.1 msec and mean conduction velocity 0.8 m/sec, with collision between orthodromic and antidromic potentials. Antidromic excitation of neuroendocrine cells was followed by inhibition of spontaneous discharge of cells for 80-125 msec suggesting the presence of recurrent collaterals in primate neuroendocrine cells. Seventy-two (57%) of the magnocellular neuroendocrine cells we designated as ;non-identified' because of lack of response, failure of collision or non-tested to pituitary gland stimulation. None of these mangocellular neuroendocrine cells, ;identified' or ;non-identified', responded to non-noxious arousing sensory stimuli.5. We find seventy-nine (63%) magnocellular neuroendocrine cells in the supraoptic nucleus (n.s.o.) and thirty (24%) magnocellular neuroendocrine cells in the internuclear zone (i.n.z.) with three basic patterns of spontaneous activity: ;silent' cells (s., four cells, 3%); ;low-frequency burster' cells (l.f.b., twenty-six cells, 21%); and ;continuously active' cells (c.a., seventy-nine cells, 63%).6. Twenty-six (21%) l.f.b. magnocellular neuroendocrine cells exhibited regular, repetitive, periodic firing patterns with a cycle of 17 sec, involving 5 sec of discharge at 5 spikes/sec and 12 sec of silence. The 26 spikes/burst showed no consistent pattern of serial interspike interval distribution. The burst pattern of discharge was interrupted by intracarotid osmotic stimuli but not by mildly arousing sensory stimuli or sleep-waking behaviour. Mean antidromic latencies of 7.98 msec and the anatomical distributions were the similarities exhibited by the l.f.b. and the c.a. cells.7. We conclude that the three functional types, s., c.a. and l.f.b., of the magnocellular neuroendocrine cells, are randomly distributed in the supraoptic nucleus (n.s.o.) and in the internuclear zone (i.n.z.) with each type receiving ;specific' input connexions. We suggest that these three characteristic firing patterns of magnocellular neuroendocrine cells may be related to the ;cellular' secretion of ;specific' neurohypophysial hormones and neurophysins.


One stage cardiac denervation without transplantation.


Ultrastructure of selected bacteria isolated from dental plaque as revealed by freeze-etching.


Fluoride concentration in root surfaces and alveolar bone of fluoride-immersed monkey incisors three weeks after replantation.


Electron microscopic study of the effect of water jet lavage devices on dental plaque.


Prevention of pacemaker infections.

Prevention of pacemaker infections.


Skeletal scintigraphy. I.

Skeletal scintigraphy. I.


Electron and x-ray beam characteristics of the linac LMR-13.


Proceedings: Hughlings Jackson Lecture. Cortical localization and "sensori motor processes" at the "middle level" in primates.


Effect of preoperative radiotherapy on the growth of human renal carcinoma tissue in vitro.


Maximal cardiac output during exercise in patients with coronary artery disease.


Transferrin concentration in human amniotic fluid.


The implications of hyperammonemia in rare and common disorders, including migraine.


Postmortem formation of giant endothelial vacuoles in Schlemm's canal of the monkey.


Ultrastructural studies of the blood-aqueous barrier. 2. The barrier to horseradish peroxidase in primates.


Assessing the costs and benefits of anti-malaria programs: the Indian experience.


The Ausria test: critical evaluation of sensitivity and specificity.


The role of red cell energy metabolism in the generation of irreversibly sickled cells in vitro.


Polygraphic studies of the effect of nitroglycerin in patients with ischaemic heart disease.


Correspondence: The quantitative determination of immunoglobulins by radial diffusion techniques.


Immune response to a syngeneic rat tumour: evolution of serum cytotoxicity and blockade. The development of serum factors by Wistar rats during the growth of a syngeneic squamous cell carcinoma has been investigated to clarify the nature of the local lymphocyte anergy reported previously in this system. Sera from tumour bearing animals were tested for cytotoxicity against tumour cells by in vitro microassay, and their ability to inhibit cell mediated cytotoxicity was also studied. Serum cytotoxicity was first detected after 2 weeks of tumour growth, reaching a peak at 4 weeks and then declining. Inhibitory activity was found only in the sera of animals with advanced tumours. Anti-tumour antibody either in the sera or bound to tumour cells was not detected by immunofluorescence techniques. No evidence of general immunological debilitation was found, the animals showing normal immune responses to sheep erythrocytes and killed Brucella abortus organisms throughout tumour growth. Serum inhibitory factors may be responsible for the decline in anti-tumour immunoreactivity and the local lymphocyte anergy observed in tumour bearing hosts.


Waldenstrom's macroglobulinemia presenting as a pulmonary mass.


Hydroxyproline index in malnutrition.

Hydroxyproline index in malnutrition.


Ossifying lipofibroma of the median nerve.


Immunological aspects of schistosomiasis. A review.


Further studies on Hepatitis Associated Antigen in acute hepatitis in Upper Egypt.


A preliminary report on the differentiation of bloods of avian species.


Editorial: Steroid induced peptic ulcer.


Studies on sperm capacitation. I. The relationship between a guinea-pig sperm-coating antigen and a sperm capacitation phenomenon.


Proceedings: The use of drugs to elucidate the mechanism of luteolysis in the pseudopregnant rat.


Cancer of the gastrointestinal tract. II. Esophagus: treatment--localized and advanced.


Variations in glomerular ultrastructure in rat kidneys fixed by perfusion.


Cancer of the gastrointestinal tract. II. Esophagus: Treatment--localized and advanced. Value of radiation therapy.


Cancer of the gastrointestinal tract. II. Esophagus: Treatment--localized and advanced. Innovations in radiation therapy.


Significance of serologic abnormalities in lepromatous leprosy.


Problems of mutation detection in independently evolving laboratory strains.


Hypothesis of post-recombination resynthesis of gene copies.


Antigenic differences between isolates of swine vesicular disease virus and their relationship to Coxsackie B5 virus.


Association of alpha-macroglobulins with lymphoid cells.


An antenatal diagnosis of spina bifida in the Lewis rat.


Suppression of TRH-mediated thyroidal release of 131-I by a synthetic analog.


The absorption of amoxycillin.

The absorption of amoxycillin.


Time for a change?

Time for a change?


The differential diagnosis of traumatic lesions of the occipito-atlanto-axial segment.


Treatment of epilepsy with clonazepam (Ro 5-4023).


Malignant degeneration of a peripheral neurofibroma with pitfalls in angiographic diagnosis.


Studies on recombination within the mouse H-2 complex. I. Three recombinants which position the Ss locus within the complex.


Raising leucocyte alloantisera in limited populations of sub-human primates.


A comparison of vaginal tampon tests used in the eradication of brucellosis.


Brucellosis in bulls.

Brucellosis in bulls.


Metabolism of 6-chloro-9 beta, 10 alpha-pregna-1,4,6-triene-3,20-dione in rat, rabbit, monkey and man.


Sluice flow in placenta: maternal vascular pressure effects on fetal circulation.


Serum IgG, IgM, and IgA levels in ankylosing spondylitis.


Studies on the transcobalamins.

Studies on the transcobalamins.


Enhanced plasma thyrotrophin response to thyrotrophin-releasing hormone following oestradiol administration in man.


Test for carcinogenicity of food additives and chemotherapeutic agents by the pulmonary tumor response in strain A mice.


Serum thyrotrophin response to thyrotrophin-releasing hormone in normal children and in patients with short stature and various endocrine or genetic diseases.


The effect of thyrotrophin-releasing hormone on plasma prolactin and thyrotrophin levels in primary hypothyroidism.


Genetic marker systems in arctic populations  VII. Genetic variation in serum lipoproteins in Icelanders.


Phenotypic bioassay of five tribes of Orissa, India.


Ag (x,y,a1,z,t) antigens in Australian and New Guinea populations.


Further experiments on the role of the nucleolus in the transfer of RNA from nucleus to cytoplasm.


Electron-microscopic observations on the macronuclear development of Stylonychia mytilus and Tetrahymena pyriformis (Ciliophora-Protozoa).


Contrasting incidence of Rocky Mountain spotted fever in ticks infesting wild birds in eastern U.S. Piedmont and coastal areas, with notes on the ecology of these ticks.


Tuberculous cervical lymphadenitis (scrofula).


A specific effect of mimosine on melanoma cells.


Activity and some properties of an acid proteinase from normal and Plasmodium berghei-infected red cells.


Antigenicity of fractions of a somatic antigen of Trypanosoma cruzi.


Hematological changes in white-tailed deer fawns, Odocoileus virgianianus, infested with Theileria-infected lone star ticks.


Development of Sarcocystis fusiformis in calves infected with sporocysts from dogs.


Asymptomatic gonorrhea in men. Diagnosis, natural course, prevalence and significance.


Studies on the cultivation of mammalian cell lines in a serum-free, chemically defined medium.


Fatty liver, alcoholic hepatitis and cirrhosis produced by alcohol in primates.


The new national health spending policy.


Meningococcal pericarditis without meningitis presenting as tamponade.


Letter: Tumor-associated antigen in human meningioma.


Characteristics of Herpesvirus saimiri-induced lymphoma cells in tissue culture.


The structure of the chicken egg shell and shell membranes as studied with the scanning electron microscope and energy dispersive x-ray microanalysis.


Interaction of H+ and Ca++ in the regulation of local pial vascular resistance.


Parameters of infection in chicks exposed to Marek's disease by two different methods.


The use of the chromium complex of ethylenediaminetetra-acetic acid for studies of digestion  in sheep.


Some effects of diet with oral contraceptives on carbohydrate:lipid metabolism in the baboon.


Biochemical characteristics of different forms of protein-energy malnutrition in rats.


Plasma amino acid concentrations and ratios in different forms of protein-energy malnutrition in rats.


Abnormal behavior in non-isolate-reared rhesus monkeys.


3 methoxytyramine: a possible endogenous toxin of psychosis?


Folate studies in underprivileged children with epilepsy.


Pseudomonas aeruginosa cervical diskitis with chondro-osteomyelitis in an intravenous drug abuser.


Summary of recent abstracts. IV. Trypanosomiasis.


A case of generalised avian tuberculosis in a horse.


The immuno-diffusion method for the identification of cattle vaccinated with Brucella abortus strain 45-20.


Determination of lidocaine in blood and tissues.


Betamethasone 17-valerate by aerosol in childhood asthma.


Thermal insulation in malnourished Jamaican children.


In vitro stimulation of lecithin synthesis in rat liver mitochondria and microsomes after treatment with phospholipase C.


Insulin degradation. X. Identification of insulin degrading activity of rat liver plasma membrane as glutathione-insulin transhydrogenase.


A manganese-stimulated endonuclease from Bacillus subtilis.


Involvement of the biosynthetic pathway of purine nucleotides in the repression of bacterial sporulation.


Temperature dependent permeability changes of Bacillus subtilis and incorporation of nucleotides into DNA.


A new approach to the characterization of the B and A forms of DNA by I.R. spectroscopy.


Differences in susceptibility to phospholipase C of free and membrane-bound phospholipids of Mycoplasma hominis.


The effect of fluoride and molybdate on caries activity in monkeys (Macaca fascicularis).


Immunologic studies on nucleic acids and their components. I. An analysis of the specificity of anti-deoxyadenylate antibodies by a membrane-binding technique.


The administration's program for the aged.


Changing life styles in housing for the aged.


Dietary management of renal failure in infants and children. Principles of nutritional therapy and some representative diets.


The effects of low concentrations of actinomycin D upon nucleic acid synthesis in different cell types.


Effects of male paragonial gland secretion on sexual receptivity and courtship behaviour of female Drosophila melanogaster.


Structure of cephalic abnormalities in Drosophila melanogaster (Diptera: Drosophilidae).


Isolation and characterization of symbiotes from the Rocky Mountain wood tick, Dermacentor andersoni.


Reproduction in two species of spider monkeys, Ateles fusciceps and Ateles geoffroyi.


Rho (d) immune globulin (human).

Rho (d) immune globulin (human).


Human placental lactogen (HPL) levels in abnormal pregnancies.


Scanning electron microscopy of the primate retina.


Treatment of pterygia with and without thiotepa.


Exercise tolerance and physical training of non-selected patients after myocardial infarction.


Phenol oxidase activity in brain tissue.


Prostaglandins and cannabis. II. Inhibition of biosynthesis by the naturally occurring cannabinoids.


Enzyme kinetics in relation to enzyme inhibitors.


Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction.


The metabolism of glyceryl trinitrate by liver and blood from different species.


In vitro inhibition of the biosynthesis of a prostaglandin by gold and silver.


Polyamines and polyamine-metabolizing enzyme activities in human semen.


Chagas enteropathy.

Chagas enteropathy.


Competitive growth of Salmonella and Pseudomonads in tetrathionate enrichment broth.


An evaluation of commercially supplied Transgrow and Amies media for the detection of Neisseria gonorrhoeae.


Sensitivity of radioimmunoassay for hepatitis B antigen in diagnostic and survey work.


Non-specific binding of IgG to antibody-coated red cells. (The 'Matuhasi-Ogata phenomenon').


Contralateral epididymo-orchitis after cryo-injury to the male rabbit gonad.


Physiology and pharmacology of local anesthetic agents.


Feed sources and conditions conducive to production of aflatoxin, ochratoxin, Fusarium toxins, and zearalenone.


Passage of bovine serum albumin from the mother to rabbit blastocysts. I. Passage from the circulation to uterine lumen.


Passage of bovine serum albumin from the mother to rabbit blastocysts. II. Passage from uterine lumen to blastocyst fluid.


A note on the purification of follicle-stimulating and luteinizing hormones from baboon pituitaries.


Effects of cooling dentate nucleus on tracking-task performance in monkeys.


Eye movements evoked by cerebellar stimulation in the alert monkey.


Effects of cooling interposed nuclei on tracking-task performance in monkeys.


Experimental comparison of radioactive agents for studies of the placenta.


Cold-sensitive Pseudomonas RNA polymerase. I. Characterization of the host dependent cold-sensitive restriction of phage CB3. Analysis of bacteriophage CB3 infection of Pseudomonas aeruginosa strain PAT2 establishes that phage induced changes in net macromolecular synthesis are absent at nonpermissive phage growth temperatures (32 C). Alterations which are evident in the PAT2 strain at 37 C or in the fully permissive strain, PAO1C, at either warm or cold temperatures do not occur in PAT2 at low temperatures. CB3 DNA synthesis and the degradation of host DNA to approximately 78S components occur at 37 C, but are absent in PAT2 at 20 C. Nevertheless, attachment of phage DNA to host cytoplasmic material occurs under permissive and nonpermissive conditions. This binding of phage DNA at 20 C is identical in nature to phage DNA bound at 37 C. Thus, the conditional cold-sensitive PAT2 host function in the growth of CB3 is expressed subsequent to membrane binding of the infecting genomes but prior to the onset of the initiation of CB3 DNA synthesis, the inhibition of host DNA synthesis, and the transient depression in RNA synthesis which occurs in permissive cells.


Cold-sensitive Pseudomonas RNA polymerase. II. Cold-promoted restriction of bacteriophage CB3 and the lack of host-dependent bacteriophage-specific RNA transcription. Cold-sensitive restriction of Pseudomonas phage CB3 by Pseudomonas aeruginosa strain PAT2 involves some aspect of CB3 specific RNA synthesis at 20 C. Experiments using chloramphenicol treatment and RNA-DNA hybridization establish that the amount of CB3 RNA present at 20 C is consistent with the known percentage of phage yielder cells at 20 C. Thus, it appears that nonyielder cells of PAT2 synthesize little or no phage-specific mRNA. Burgess technique extracted PAT2 RNA polymerase (RNAP) is cold sensitive when assayed in vitro with CB3 DNA at 20 C. However, it is not cold sensitive when either calf thymus or PAT2 DNA are the templates for transcription. Low ionic strength assay conditions eliminate the cold sensitivity of PAT2 RNAP. The effect of low ionic environments on transcription initiation along with the in vivo and in vitro suppression of cold sensitivity by host rifampin resistance suggests that the inability of CB3 to reproduce in PAT2 at 20 C is a cold-sensitive step in host RNAP initiation. Our modified RNAP extraction procedure for PAT2 and PAO1C also results in the recovery of cold-sensitive PAT2 RNAP with respect to CB3 DNA templates and points to basic enzymological differences between the two hosts. A model is presented for the unusual influence of temperature on the initiation process of both PAT2 and PAO1C on RNAP transcription.


Antigenic properties of bacteriophage phi 29 structural proteins. Serological methods and electron microscopy were used to study the structural proteins of the small Bacillus subtilis bacteriophage phi29. This virus has a large number of fibers attached at both ends of its prolate head. A complex neck assembly is comprised of 12 symmetrically arranged appendages as the outer component. Head fibers, neck appendages, and the head surface bind anti-phi29 antibodies. Immune sera absorbed with defective lysates of suppressor-sensitive (sus) mutants have been used to determine the genetic control of neck appendages production. Studies on the serum-blocking power of lysates defective in different tail components showed that appendages contain the main serum-blocking protein. This finding suggests an essential role of the neck appendages in phage adsorption or DNA injection.


Isolation of skyrin from Hypomyces iactifluorum.


The secretion of migration inhibitory factor by intact schistosome egg granulomas maintained in vitro.


A comparison of the cardiovascular actions of thalicarpine and its moieties.


Mechanism of action of L-asparaginase on the cell cycle and growth in acute lymphoblastic leukemia.


The effect of L-asparaginase on DNA and RNA synthesis by lymphoblasts of acute lymphocytic leukemia.


Development of the auditory cortex of monkeys during prenatal ontogenesis.


Lymphocyte surface markers in lymphoproliferative disorders.


Binding of folic acid to serum proteins. II. The effect of diphenylhydantoin treatment and of various diseases.


The prevalence of Australia antigen and antibody in haemophilia.


Blood group and tissue mosaicism in a Natal Indian woman.


Acquired factor IX deficiency. A report of two cases.


Haemoglobin D Punjab in a Cuban family and its interaction with haemoglobin S.


Human simulated diving experiments at saturation under oxygen-helium exposures up to 500 meters: electroencephalographic data.


Electron microscopic study of endothelium of stored and cryopreserved monkey corneas.


Infection control in intravenous therapy.


Interference between borreliae and trypanosomes. I. Antigenic analysis, fluorescent antibody and immobilisine studies of Borrelia turicatae and Trypanosoma cruzi.


Studies on the life cycle of Phaneropsolus bonnei and Prosthodendrium molenkampi in Thailand.


Chromosomal proteins of Drosophila embryos.


Fetal hormones, the brain, and human sex differences: a heuristic, integrative review of the recent literature.


Microheterogeneity of rat liver ferritin: comparison of electrofocusing and chromatographic fractions.


The binding affinities of rheumatoid factors interacting with the C gamma 3 homology region of human IgG.


Immunological and physicochemical studies of IgA1 (delta) cryogelglobulinaemia.


Scoliosis.

Scoliosis.


Proceedings: The preservation of ciliated protozoa at low temperature.


Proceedings: Factors affecting repair of sublethal injury in frozen or freeze-dried bacteria.


Proceedings: Freezing and freeze-drying of Spirulina platensis.


Diagnosing thyroid disease by selected laboratory tests.


Lidoflazine: double-blind trial of a new coronary vasodilator in angina pectoris.


The effects of temporary protein deprivation on bone width and bone mineral content in rhesus macaques.


Degradation of coxsackievirus type A9 by proteolytic enzymes. The means by which coxsackievirus type A9 (CA9) is inactivated by proteolytic enzymes was investigated. After reaction of (14)C-leucine-labeled CA9 with Pronase, free leucine was liberated as measured by radiochromatography. Treatment of (14)C-leucine-labeled CA9 with trypsin or proteolytic filtrates of Pseudomonas aeruginosa caused the release of a variety of labeled substances. The extent of viral ribonucleic acid (RNA) release after exposure of CA9 to Pronase was determined by RNA infectivity tests or trichloroacetic acid solubility tests. Infective viral RNA was found not to be consistently released by reaction of CA9 with Pronase, but further treatment with 1% sodium dodecyl sulfate at pH 7.0 promoted viral RNA release. Sodium dodecyl sulfate treatment of CA9 that had not been reacted with Pronase did not inactivate virus or cause viral RNA release. Reaction of Pronase with (32)P-labeled CA9 resulted in the liberation of virus components soluble in cold trichloroacetic acid, whereas untreated CA9 or CA9 reacted with ribonuclease were precipitated by cold trichloroacetic acid. These results demonstrate that the primary means by which protease-sensitive enteroviruses are inactivated is by degradation of the virus capsid, with subsequent release of viral RNA.


Immunocyte response to experimental mumps virus infection in Rhesus monkeys. Nineteen rhesus monkeys were inoculated with mumps virus by retrograde ductal instillation into the parotid gland. Evidence of infection was obtained in all instances. Virus was isolated from buccal swabbings and parotid biopsies for 1 week after inoculation. A vigorous serum-neutralizing antibody response occurred within 3 weeks, and there was marked monocytic infiltration of the parotid stroma. The monocytic infiltrate comprised as much as 60% of the total gland volume 1 week after infection. The predominant inflammatory cells were non-immunoglobulin-containing mononuclear cells resembling lymphocytes. Plasma cells containing immunoglobulin G (IgG), IgA, IgM, and IgE increased in numbers in the gland after infection, the greatest increase occurring in IgG-containing cells. Neutralizing antibodies and interferon were found in extracts prepared from the infected glands. Neutralizing activity was highest in samples taken 3 weeks after infection but was detectable in samples taken as soon as 36 to 48 h after infection. Interferon activity was detected in significant amounts 36 to 48 h after infection. Challenge of previously infected animals resulted in an increase in the monocytic infiltrate as well as an increase in numbers of immunoglobulin-containing plasma cells. However, reinfection did not occur as evidenced by the inability to culture shed virus after challenge. This model should be useful for in vivo study of biochemical mediators which evoke inflammatory cell infiltration and which may be significant both in protection and in tissue damage.


Mechanism of Shope fibroma virus-induced suppression of host deoxyribonucleic acid synthesis. The effects of treatment with live or inactivated Shope fibroma virus on host cell deoxyribonucleic acid (DNA) synthesis were determined. The incorporation of (3)H-thymidine into nuclear DNA was suppressed by both active and inactivated virus, although live virus was more effective. During the early phase of infection, stimulation of host nuclear DNA synthesis of up to 240% of control value was observed in cells infected with active virus. Inhibition of DNA synthesis began at about the 8th h and was maximal by 12 h postinfection. Virus inactivated by ultraviolet-irradiation or heat treatment did not induce viral DNA synthesis but was, nevertheless, able to suppress host DNA synthesis.


Rickettsial hemolysis: effect of metabolic inhibitors upon hemolysis and adsorption. The hemolysis of sheep red blood cells by typhus rickettsiae is initiated by a temperature-sensitive adsorption process that is dependent on the energy-generating metabolism of the rickettsaie but not that of the erythrocyte. Adsorption is followed by events dependent on the metabolism of both the rickettsaie and red blood cells. At 34 C the adsorption step is complete after about 4 min, at lower temperatures the time required for adsorption is increased. At 0 C no adsorption occurred. The addition of cyanide inhibited the catabolism of glutamate by the rickettsiae and, consequently, both adsorption and the subsequent steps in hemolysis. The starvation of the rickettsiae for glutamate also prevented adsorption. Fluoride had no demonstrable effect on rickettsial metabolism nor the adsorption step but inhibited glycolysis in the erythrocytes and the hemolysis of the erythrocytes by rickettsiae.


Unique teichoic acid isolated from the cell walls of a strain of Staphylococcus aureus. Teichoic acid antigen extracted from cell walls of a strain of Staphylococcus aureus has been shown to have the unique structure of glycerolphosphate, with N-acetyl-d-galactosamine as the immunodominant substituent.


Antibody response to chlamydia agents: lack of immunoglobulin M antibodies during the secondary immune response. Intramuscular inoculation of the monkey with a massive dose of Chlamydia agent particles resulted in the production of high levels of immunoglobulin M antibodies. These antibodies were broadly specific and reacted to high titers with heterologous antigens. Challenge inoculation of the same monkeys with heterologous Chlamydia agent particles resulted in the production of increased levels of immunoglobulin G but not immunoglobulin M antibodies. Some immunological implications of these results are discussed.


Effects of endotoxin on gluconeogenesis, glycogen synthesis, and liver glycogen synthase in mice. This study was undertaken to characterize the nature of carbohydrate loss due to endotoxin poisoning in mice and to elucidate mechanisms responsible for the changes. Female ICR mice, fasted overnight, were injected intraperitoneally with a mean lethal dose of endotoxin extracted from Salmonella typhimurium strain SR-11. Liver glycogen levels, alanine-U-(14)C and pyruvate-2-(14)C incorporation into blood glucose and liver glycogen, glucose-U-(14)C incorporation into liver glycogen, and liver glycogen synthase activities were measured at intervals after treatment. Liver glycogen in fasted mice given endotoxin was diminished significantly as early as 1 h after treatment. Liver glycogen synthase was significantly decreased in poisoned mice at 17 h. The use of actinomycin D showed that the induction of this enzyme due to fasting or hydrocortisone, or both, was inhibited by endotoxin. The incorporation of the (14)C-label from alanine-U-(14)C, pyruvate-2-(14)C, or glucose-U-(14)C into blood glucose and liver glycogen was substantially impaired in endotoxemic animals at 12 h. Decreases in incorporation occurred as early as 4 h after treatment. The progressive increase in glycogen synthase activity observed in fasted controls was not seen in endotoxin-poisoned mice. The administration of a glucose or pyruvate load to endotoxin-treated mice did not restore gluconeogenesis, glycogen synthesis, or liver glycogen synthase activity to normal levels. The in vivo activation of glycogen synthase by glucose was significantly reduced in endotoxemic animals. These changes indicate reduced carbohydrate synthesis as a probable cause for rapid sugar loss during endotoxemia in mice.


Gel precipitation of common enterobacterial antigen by its antibody.


Observations on the cross-immunity between Theileria lawrencei (Serengeti) and Theileria parva (Muguga) in cattle.


Anoxic radiosensitization of two Bacillus species by p-nitroacetophenone.


Influence of cysteamine on intramolecular energy transfer in 5-bromouracil-substituted phage-DNA.


Phagocytic and bacterial properties of normal human monocytes. The bactericidal and phagocytic capacities of monocytes for E. coli, Staphylococcus, Salmonella, and Listeria, and factors that influence these functions were evaluated and compared with those of the polymorphonuclear leukocytes of 30 normal human subjects. Monocytes killed a significantly smaller proportion of each of the bacterial species than did neutrophils from the same individuals. Whereas the neutrophils of all individuals demonstrated the ability to kill significant numbers of the four bacterial species, there was a marked variation in the effect of monocytes of different individuals on the growth curves of these same bacteria. When the bactericidal capacity of an individual's monocytes to more than one species of bacteria was examined in the same experiment, a significant difference in the effect of monocytes on the growth curve of one bacterial species as opposed to another was noted in 4 of 17 subjects. The bactericidal ability of monocytes of single individuals was consistent on different days in 9 of the 11 subjects whose monocytes were examined more than once against the same bacteria. Studies were performed to determine if the lesser bactericidal capability of monocytes was due to a difference in the ability of monocytes and neutrophils to phagocytize or to a difference in the ability of these cells to kill ingested bacteria or both. The results demonstrated that monocytes phagocytize bacteria significantly less well than neutrophils, but the intracellular killing capacity of both cell types is equal. Addition of phenylbutazone to cell suspensions completely inhibited intracellular killing by both monocytes and neutrophils, suggesting the possibility that the bactericidal mechanisms in both cell types might be similar. Monocyte killing of E. coli, Salmonella, and Listeria, but not of Staphylococcus, was significantly diminished in heat-inactivated autologous serum. Neither increasing the concentration of autologous serum from 10% to 25% nor replacement of autologous serum with pooled human serum had any effect on monocyte killing of any of the four bacteria. These studies demonstrate that peripheral blood monocytes are less bactericidal for the four bacterial species than neutrophils, solely because monocytes are less phagocytic. A baseline for further study of factors that influence monocyte function and for study of this cell in selected patient populations is provided.


Studies on derivation of transcobalamin 3 from granulocytes. Enhancement by lithium and elimination by fluoride of in vitro increments in vitamin B12-binding capacity. Unsaturated vitamin B(12)-binding capacity (UBBC) of human serum is not reproducibly measurable because it increases variably in vitro in relation to time, temperature, and, in the case of plasma, anticoagulant present before removal of cells. This variable increase proved to be due to variable release in vitro of transcobalamin III (TC III) from granulocytes. UBBC increase was greatest (up to fourfold normal levels) in the presence of lithium, which is the heparin salt used in many laboratories doing UBBC studies. In vitro increase was least when blood was collected in EDTA at 0 degrees C and immediately centrifuges at 0 degrees C (T(0) sample); results equivalent to T(0) were obtained at room temperature even after several hours delay when 47 mM fluoride was present; either cold temperature or 47 mM fluoride appeared to prevent TC III release from granulocytes. The measured levels of the three transcobalamins with T(0) methods of collection, which presumably reflect most closely the in vivo circulating levels, suggest that TC I and TC III in normal plasms are of the same order of magnitude and together normally comprise less than 10% of the UBBC. Approximately 90% of the UBBC content of sonicates of peripheral blood granulocytes and of bone marrow aspirates of normal individuals appears to be TC III, with the rest being TC I. Thus, normal myelocytes, like normal granulocytes, appear to contain mainly TC III. No TC II was present in any of the sonicates. The general practice in most laboratories has been to determine serum UBBC. Because in vitro increments of up to 119% were found to occur in serum, this practice should be replaced by collection using methods that prevent such increments. Blood collected in EDTA-47 mM NaF had a stable, reproducible UBBC with no significant in vitro increment with time.EDTA-NaF UBBC was 640+/-168 (range 380-921 pg B(12) bound/ml plasma) for 12 normal adult men and 809+/-232 (range 505-1208) for normal adult women. It presumably approximates circulating UBBC and is substantially below the serum UBBC mean of 935+/-262 (range 611-1506 for the same 12 men) and 1273+/-355 (range 811-2306 for the same 10 women).


Enzymatically inactive red cell carbonic anhydrase B in a family with renal tubular acidosis. An inactive mutant form of red cell carbonic anhydrase B is described in three members of a large kindred who manifest infantile renal tubular acidosis and nerve deafness. A combination of enzymatic and immunologic investigations permitted its detection, despite the fact that both antigenic and electrophoretic properties of the mutant were identical to those of the normal form.


Direct radiochemical assay for serum folate: competition between 3H-folic acid and 5-methyltetrahydrofolic acid for a folate binder.


Antibiotic levels in pericardial fluid.

Antibiotic levels in pericardial fluid. An experimental model was designed to study the ability of antibiotics to enter the pericardial compartment. Noninfected and infected pericardial fluid and serum antibiotic activities were determined in adult mongrel dogs before and at intervals after antibiotic administration. After the administration of penicillin G, methicillin, cephaloridine, streptomycin, or gentamicin, clinically adequate antibiotic levels in the noninfected pericardial fluid were obtained within 1 h, and these levels approached or exceeded the serum levels within 2-4 h. Antibiotic levels obtained from infected dog pericardial fluids were higher than those from noninfected animals. Patients' serum and pericardial fluid antibiotic levels were measured after penicillin G, penicillin V, cephalothin, and gentamicin administration. We have found, both in the canine and human studies, that pericardial antibiotic levels taken at least 2 h after antibiotic administration are almost identical to those in the blood.


Hepatic bilirubin glucuronidation in neonates with unconjugated hyperbilirubinemia and congenital gastrointestinal obstruction.


The role of medical education in planning the development of a national health care system.


Some good features of the British National Health Service.


The world of health and the American physician.


Supervoltage isocentric arc therapy in carcinoma of the bladder.


Paper chromatographic determination of tetracycline.


Nitroglycerin sublingual tablets. II. Preparation and stability of a new, stabilized, sublingual, molded nitroglycerin tablet.


Synthesis and biological activity of quinolyl acetic acid derivatives.


Enhancement of benzalkonium chloride and chlorhexidine acetate activity against Pseudomonas aeruginosa by aromatic alcohols.


Optimal criteria for care of patients with cancer.


Gram-negative infections in cancer. Study of empiric therapy comparing carbenicillin-cephalothin with and without gentamicin.


Pneumonia caused by Neisseria meningitidis.


Serum proteins and gestational age.

Serum proteins and gestational age.


Neutron radiographic examination of soft-and hard-tissue structures of the oral cavity.


Research in neurosis: a selective review. 1. Causes and courses.


On the mechanism by which bovine fibrinogen induces the release and availability reaction of human blood platelets.


Immunological studies on Egyptian polyvalent antivenins.


Phospholipase isoenzymes from Naja naja venom. I. Purification and partial characterization.


Antiendotoxin effect of disodium cromoglycate.


Blood parasites of the Impala (Aepyceros melampus) in the Serengeti National Park.


PCB residues in plankton from the Gulf of St Lawrence.


Distribution and persistence of polychlorinated biphenyls (Aroclor 1254) in growing piglets.


Congenital defects of the corpus callosum. Morphology and encephalographic appearances.


A clinical study of the benzodiazepine Ro 5-4023 (clonazepam) in the treatment of epilepsy.


Drug susceptibility-resistance pattern of gram-negative uropathogens to seven cephalosporins.


Analytical methodology for bioactive compounds. Photochemically assisted analysis of chlorinated hydrocarbon pesticides in the presence of polychlorinated biphenyls.


Microdilution antibiotic susceptibility test: examination of certain variables. A semiautomated microdilution susceptibility test is described. The effect of certain parameters such as inoculum size, growth media, incubation conditions, and inoculum dispensing systems was studied. Both medium type and inoculum size caused significant variations in the minimum inhibitory concentrations (MIC) of certain antibiotic-organism combinations. No effect on MIC was observed as a function of incubator type. Efforts to read a reproducible MIC value in less than 12 h failed. A commercially available wire pronged inoculator was determined to be inaccurate and unsafe. Disposable dropper pipettes proved to be economical, accurate, and precise. Although a standard method for microdilution antibiotic susceptibility testing is not proposed, data are presented which show that future attempts at standardized procedures are mandatory if inter- and intralaboratory reliability is desired.


Development and evaluation of a direct fluorescent antibody method for the diagnosis of Pneumocystis carinii infections in experimental animals. Because no fully satisfactory diagnostic method has been available for use in pneumocystis infection, an attempt was made to apply the fluorescent antibody technique in the identification of Pneumocystis carinii. Hyperimmune sera were prepared in rabbits against P. carinii from human and rat sources. After proper adsorption, these antisera were conjugated with fluorescein isothiocyanate and used as reagents in a direct fluorescent antibody procedure. Each of the two reagents was found to stain trypsin-treated P. carinii organisms from either human or rat sources, indicating the presence of common antigens. Stained organisms were demonstrated in the hypopharyngeal material from rats in which pneumocystis infection had been activated by the administration of corticosteroid. From the results reported here, the procedure outlined is considered sufficiently sensitive and specific to justify tests on pneumocystis infections in man. The findings in a series of specimens from human subjects will be reported separately. The method also provides an extended approach to related research problems. The need for controls of the procedure at all points is emphasized.


Method for the selective enumeration of blue-green bacteria in water. A membrane filter method for the selective enumeration of blue-green bacteria has been developed which, on the basis of studies with laboratory cultures and field tests, has proved to be both practical and reproducible. The filters are incubated under specified conditions of temperature and illumination on a mineral salts agar medium supplemented with yeast extract and containing cycloheximide to eliminate eukaryotic contaminants.


Effects of changes in feed level, starvation, and level of feed after starvation upon the concentration of rumen protozoa in the ovine. Four rumen fistulated sheep were used in five experiments to investigate the effect of feed level upon the concentration of rumen ciliate protozoa. The sheep were fed once daily 650 g of a pelleted diet composed of corn cobs, 45%; alfalfa meal, 35%; oats, 12.5%; cane molasses, 5%; urea, 0.4%; and vitamins and minerals, 2%. The concentration of protozoa reached minimum and maximum values at 5 and 22.5 h after feeding, respectively. Thus, to estimate apparent generation rates, concentrations of protozoa were determined at 5 and 20 h postfeeding. Apparent generation rate/h = natural log of ([concentration of protozoa at 20 h divided by concentration at 5 h] divided by the time interval, [T20 to T5]). Alteration of the feed to protozoa ratio by starvation and by changing the level of feed (200 to 900 g/day) showed that as the ratio of feed to protozoa increased, generation rate increased. Measurements of liquid turnover rates in the rumen showed that turnover rate decreased as feed level decreased. Turnover rate was near zero when the sheep were starved. Small quantities of soluble substrates, added directly to the rumen of starved sheep, maintained the protozoal population when rumen turnover was minimal. Furthermore, as rumen turnover rate increased with increased levels of feed, the effect of substrate on maintaining the protozoal population was negated. Thus, at high feed levels, turnover rate may be the dominant factor controlling the establishment and concentration of protozoa in the rumen.


Effect of propylene oxide treatment on the microflora of pecans. Studies were conducted to determine the effectiveness of propylene oxide (PO) treatment in controlling the microflora of pecans. As used commercially, PO has little effect on internal bacteria and fungi in pecan halves. Tests of surface washings of commercially treated pecans showed a reduction of 96% in coliform bacteria following PO treatment. Under controlled laboratory conditions, PO gave 80 to 92% reduction of surface microflora and at least 64% reduction of internal flora, but neither bacteria nor fungi could be eliminated completely, even with high dosages. Current assay methods for determining bacterial content of nutmeats were shown to be inadequate because they utilize only surface washings and thus do not give an accurate picture of the total bacterial population of the nutmeat. Consequently, such assays do not permit an accurate assessment of any potential health hazard related to these organisms.


New toxin from Aspergillus flavus.

New toxin from Aspergillus flavus. Two nonaflatoxin-producing isolates of Aspergillus flavus produced a new nonfluorescent nitrogen-containing metabolite that was highly toxic to 1-day-old cockerels. The oral mean lethal dose of toxin was 19 mg/kg. Chemical and physical data obtained on the purified toxin demonstrated that it was not one of the previously reported metabolites of A. flavus. The common name "flavutoxin" has been assigned to the toxin.


Distribution of pyrimidine oligonucleotides in complementary strand fractions of Escherichia coli deoxyribonucleic acid.


Characterization of two plant lectins from Ricinus communis and their quantitative interaction with a murine lymphoma.


Carbonic anhydrase catalyzed hydrolysis and decarboxylation. Kinetic studies of enzyme-catalyzed decomposition of mono- and disubstituted derivatives of carbonic acid.


Multiple forms of galactosyltransferase from bovine milk.


Human erythrocyte phosphoglycerate kinase: purification, properties, and interaction with its antibody.


Purification and some properties of rat spleen phospholipase A.


The effects of Ca2+ on procine enteropeptidase activity.


Inactivation of infectious deoxyribonucleic acid of bacteriophage phi chi X 174 by colicin E2.


Gel electrophoresis of RNA under denaturing conditions.


Further studies of the fluorescent treponemal antibody-cerebrospinal fluid (FTA-CSF) test with a monospecific anti-IgM conjugate.


Gonococcal pharyngeal infections. Report of 110 cases.


Neisseria gonorrhoeae identified by the direct fluorescent antibody technique in male contacts.


Studies in venereal disease. II. Improved diagnosis of gonorrhoea by the parallel use of conventional and L-phase media for culture.


Asymptomatic meningococcal urethritis. Possible protective value against gonococcal infection by bacteriocin production.


Long-term triple tetracycline ("Deteclo") treatment of non-specific urethritis.


Comparison of trimethoprim-sulphamethoxazole and penicillin in the treatment of gonorrhoea.


Sialic acid content of red blood cells from protein-calorie malnourished children and during recovery, and from normal children and adults.


Editorial: Carbon monoxide, smoking and cardiovascular disease.


Fab-related fragments of human IgG produced by partial tryptic proteolysis.


Salicylamide glucuronide formation in newborns with severe jaundice of unknown etiology and due to glucose-6-phosphate dehydrogenase deficiency in Greece.


Isolation of immunochemically pure IgE from serum of E-myeloma patient Yu.


Formation of a hemolytically active cellular intermediate by the interaction between properdin factors B and D and the activated third component of complement. The present studies demonstrate that the factor B-dependent C3 convertase can be affixed to an erythrocyte by use of an intermediate bearing C3b and that this convertase brings the hemolytic reaction to completion with an efficiency comparable to that of classical convertase. The evidence that the EAC43 intermediate was lysed by a new pathway includes requirements for factors B and D and cell-bound C3b for subsequent lysis by the terminal components, C3-C9. The linear stoichiometry of the effective molecule titrations of C3b and factor B, and the first-order kinetics displayed by the generation and decay of the factor B-dependent hemolytic site are characteristics consistent with the one-hit theory as initially developed for the classical complement system. The use of hemolytically active cellular intermediates to examine the reactions occurring with C3b and factors B and D has allowed extension of the one-hit theory to this molecular sequence, development of effective molecule titrations, recognition of the analogies to the functional characteristics of the classical C3 convertase, and discrimination of the probable mechanism of terminal complement activation from reactive lysis.


Influence of erythrocyte membrane components on malaria merozoite invasion. Chymotrypsin- and Pronase-treated human erythrocytes were refractory to invasion by P. knowlesi merozoites; invasion was not inhibited by trypsin or neurammidase treatment. These data implicate a surface protein other than sialoglycoprotein as the receptor site for merozoites. Invasion of rhesus erythrocytes was unaffected by pretreatment with these enzymes. Differences in membrane structure of erythrocytes from various species may explain the absence of an enzyme effect on rhesus erythrocytes.


The usefulness of thyrotropin-releasing factor (TRF) test in patients with hypothalamic-pituitary disorders.


New aspects in the biological role of zinc: a stabilizer of macromolecules and biological membranes.


Methadone inhibition of glycogen synthase and phosphorylase in rat skeletal muscle.


A kinetic study of the participation of zinc in human spermatozoa metabolism.


Antigenic independence of the two types of alkaline phosphatases from Serratia marcescens strain 211.


HMO performance: the recent evidence.

HMO performance: the recent evidence.


Inhibition of insulin release during hypovolemic shock.


Willebrand factor: immunological and biological study.


Proceedings: Perhexiline versus prenylamine. A controlled clinical trial in coronary insufficiency.


[Proceedings: Review of the cardiovascular pharmacology of perhexiline].


Proceedings: Experience with the use of perhexiline in patients with chronic coronary insufficiency: preliminary report.


Effect of ageing on the levels of the main classes of serum immunoglobulins in active chronic hepatitis and in clinically healthy subjects.


Hog cholera (European swine fever).

Hog cholera (European swine fever).


Biphenyl-exposed rabbits.

Biphenyl-exposed rabbits.


Pharmacokinetic evaluation of penicillin and cephalosporin derivatives in serum and milk of lactating cows and ewes.


Control of magnesium-protoporphyrin chelatase activity in Rhodopseudomonas spheroides. Role of light, oxygen, and electron and energy transfer. 1. Magnesium-protoporphyrin chelatase activity, previously shown in whole cells of Rhodopseudomonas spheroides, could not be demonstrated in cell-free extracts prepared in different ways, although spheroplasts retained moderate activity. Slight activity was detected also in whole cells of Rhodospirillum rubrum. 2. The effects on the activity of the enzyme of inhibitors of electron and energy transfer were studied in whole cells of Rps. spheroides. Amytal, rotenone, azide and cyanide inhibited at low pO(2) in the dark but not under anaerobic conditions in the light. Antimycin A and 2-heptyl-4-hydroxyquinoline N-oxide, as well as uncouplers and oligomycin, inhibited under all environmental conditions. 3. The effects on magnesium chelatase activity of intermediates of the tricarboxylic acid cycle, of thenoyltrifluoroacetone, of a number of artificial electron donors or acceptors, of various quinones and of the oxidation-reduction indicator dyes Benzyl Viologen and Methyl Viologen are described. 4. It was concluded that electron transport between a b-type and a c-type cytochrome as well as associated energy conservation and transformation reactions were essential for activity. There was also a specific requirement for ATP. 5. Exogenous protoporphyrin and magnesium protoporphyrin monomethyl ester were incorporated into bacteriochlorophyll or late precursors by whole cells. 6. Evidence is presented that the insertion of magnesium was the only step inhibited by oxygen in the biosynthetic pathway between protoporphyrin and bacteriochlorophyll.


Stimulation of non-conservative DNA synthesis by ultraviolet light in freeze-treated Bacillus subtilis.


Binding of parathyroid hormone to bovine kidney-cortex plasma membranes. 1. Plasma membranes were purified from bovine kidney cortex, with a fourfold increase in specific activity of parathyroid hormone-sensitive adenylate cyclase over that in the crude homogenate. The membranes were characterized by enzyme studies. 2. Parathyroid hormone was labelled with (125)I by an enzymic method and the labelled hormone shown to bind to the plasma membranes and to be specifically displaced by unlabelled hormone. Parathyroid hormone labelled by the chloramine-t procedure showed no specific binding. (75)Se-labelled human parathyroid hormone, prepared in cell culture, also bound to the membranes. 3. Parathyroid hormone was shown to retain biological activity after iodination by the enzymic method, but no detectable activity remained after chloramine-t treatment. 4. High concentration of pig insulin inhibited binding of labelled parathyroid hormone to plasma membranes and partially inhibited the hormone-sensitive adenylate cyclase activity in a crude kidney-cortex preparation. 5. EDTA enhanced and Ca(2+) inhibited binding of labelled parathyroid hormone to plasma membranes. 6. Whereas rat kidney homogenates were capable of degrading labelled parathyroid hormone to trichloroacetic acid-soluble fragments, neither crude homogenates nor purified membranes from bovine kidney showed this property. 7. Binding of parathyroid hormone is discussed in relation to metabolism and initial events in hormone action.


Translational inhibition in extracts from serum-deprived animal cells.


Maternal age effect associated with yellow pigment in Malpighian tubes of Drosophila melanogaster.


Growth and enterotoxin B synthesis by Staphylococcus aureus S6 in associative growth with Pseudomonas aeruginosa.


Purification and characterization of Pseudomonas aeruginosa alkaline phosphatase.


Growth of Streptococcus cremoris and Streptococcus lactis in a chemostat. Production of cells and survival of bacteria during frozen storage.


Chapter 30. Current status of spinal cord cooling.


Sedimentation characteristics of thyroglobulin in some amphibians after 131-I iodination in vivo.


Thymidylate synthetase in mutants of Drosophila melanogaster. Thymidylate synthetase has been examined with respect to the normal pattern of activity throughout the development of the Oregon-R strain of Drosophila melanogaster. Large amounts of the enzyme are present in both the unfertilized and the fertilized eggs. A comparison of the ovarian thymidylate synthetase activity of the Oregon-R strain and the female sterility mutants, fs(2)B, fu, and fs(1)N, indicates variations in the activity of this enzyme in each strain. At four days of age, the ovarian-specific activity of the female sterility mutants is comparable to or less than that of the Oregon-R strain, but it is reduced at fifteen days of age. The enzyme activity per ovary is low in the fs(2)B strain but is similar in the Oregon-R, fu, and fs(1)N strains. When expressed as activity per organism, thymidylate synthetase declines after six to eight hours of development until the minimal level is reached in the late embryonic stage. Enzyme activity rises throughout the larval instars, reaching a maximum immediately after puparium formation. The activity decreases during pupation, but rises again during the first four days of adult life.


Gene conversion and transfer of genetic information within the inverted region of inversion heterozygotes. Prior studies of recombination which monitor exchange events in exceedingly short intervals (i.e., separable sites within a cistron) reveal that the basic event in recombination involves a non-reciprocal transfer of information, termed conversion. As a logical consequence of the model suggested by the work in Drosophila, the present investigation examined recombination between rosy mutant alleles (ry:3-52.0) in Drosophila melanogaster in a paracentric inversion (In(3R)P(18)) heterozygote, which placed the rosy region approximately at the center of the inverted region. Comparison of the results of this study with experiments carried out in standard chromosome homozygotes reveals a dramatic suppression of classical crossovers between the rosy mutant alleles in the inversion heterozygote. However, conversions continue to occur for all rosy mutant alleles in all heterozygous combinations in the inversion heterozygote. Moreover, the order of magnitude of conversion frequencies seen in the inversion heterozygote does not change from that seen in the standard chromosome homozygote study. The significance of these observations with reference to the role of rearrangements as barriers of information transfer is discussed. Particular attention is directed to the elaborate inversion polymorphisms seen in natural populations, and to notions concerning their role in the evolution of adaptive gene complexes.


A case of high rate of spontaneous mutation affecting viability in Drosophila melanogaster. A spontaneous lethal mutation rate approximately twenty to thirty times greater than normal has been discovered in second and third chromosomes derived from an irradiated isogenic line and paired with marked inversion chromosomes. Mutations resulting in reductions of viability of varying magnitude short of complete lethality apparently also occur at a very high rate in the third but not in the second chromosome. The pattern of accumulation of lethal mutations over several generations and viability frequency distributions within generations have been studied in a number of independent experiments. High mutation rate occurs in heterozygous isogenic-derived second and third chromosomes, either together or apart, irrespective of the genetic constitution of nonhomologous chromosomes. High mutation rates were not observed using the same methods with chromosomes of an inbred line from a different source. The possible mechanisms responsible for these results are discussed.


Segmental aneuploidy as a probe for structural genes in Drosophila: mitochondrial membrane enzymes. A method for detecting possible structural genes in D. melanogaster based on gene dosage dependency is presented. By making thirty crosses between Y-autosome translocations, and an attached-4 cross, it is possible to produce large duplications (approximately 150 salivary gland chromosome bands in length) for every autosomal region with the exception of 83DE. The usefulness of the technique was demonstrated by dosage dependency of three known gene-enzyme systems: alpha-glycerophosphate dehydrogenase-1, alcohol dehydrogenase and malate dehydrogenase. A screen for genes affecting two enzymes localized on the inner membrane of the mitochondrion, alpha-glycerophosphate oxidase (alphaGPO) and succinic dehydrogenase (SHD), produced a dosage-sensitive region in each case. Region 50C-52E affected alphaGPO activity and region 28D-29F affected SDH activity. The latter region apparently includes the malic dehydrogenase-1 gene. The methodology and limitations of the technique are discussed.


The crossveinless polygenes in an Iowa population. A natural population of Drosophila melanogaster was tested for the presence of crossveinless (cve) alleles over the course of a summer. Approximately 8% of the wild-caught females tested carried enough cve alleles to produce true-breeding crossveinless lines. In some cases, different isofemale lines produced identical cve strains. Also, differences were sometimes observed among independently selected cve sublines of a given isofemale line.-The cv-2 allele was found to occur with a frequency of about 1% to 2%.


The genetic control and biochemical modification of catechol oxidase in maize. Three isozyme variants of catechol oxidase have been shown to be determined by alleles of a gene, Cx, which has been located on chromosome 10 less than 0.1 recombination units from the endosperm marker du(1).-The extractable form of the enzyme is modified by an endogeneous "modifier" which appears to function as an enzyme substrate. Enzyme and modifier are functionally isolated in intact cells. Modified enzyme has altered kinetics, does not migrate in electrophoresis and most probably results from a "tanning" of the enzyme by reaction products. The content of modifier varies in different lines and is genetically determined by gene(s) independent of Cx. Treatment with maleic hydrazide causes a ten-fold reduction in the modifier content of seedlings, allowing the enzyme to be extracted in an unmodified form which will migrate in electrophoresis.-This system of enzyme and modifier fits the requirements of hypersensitive disease resistance in plants and may provide a test system to investigate the biochemical basis of disease resistance.


Carbonic anhydrase activity in amphibian and reptilian lung: a histochemical and biochemical analysis.


Energy-dependent release of magnesium from beef heart submitochondrial particles.


Denaturation of bovine carbonic anhydrase B by guanidine hydrochloride. A process involving separable sequential conformational transitions.


Purification and properties of a progesterone-induced basic glycoprotein from the uterine fluids of pigs.


Noninvolvement of copper in the L-tryptophan 2,3-digoxygenase reaction.


Control of metabolite secretion in Bacillus subtilis.


Chemotactic responses by motile bacteria.


The relationship between the O-antigenic lipopolysaccharides and serological specificity in strains of Pseudomonas aeruginosa of different O-serotypes.


The metabolism of 2-oxogluconate by Pseudomonas aeruginosa.


The effect of trypsin on the formation of virus-specific surface antigen in cowpox virus-infected cells.


Temperature-sensitive events in the growth of alastrim virus in chick embryo cells.


Dermatological aspects of pseudo-Hurler polydystrophy (mucolipidosis 3).


Spread of bovine syncytial virus in cell culture.


A population model for limited food competition.


Tonic communication: continual effects of discrete signs in animal communication systems.


Molecular evolution of myoglobin and the fossil record: a phylogenetic synthesis.


Immune response to C. albicans in monkeys with experimental candidiasis in the palate.


Histologic variations in the pulp of intact premolars from young individuals.


Alkaline phosphatase in the enamel organ of the rat incisor.


The metabolism of ibuprofen.

The metabolism of ibuprofen.


Demonstration of thyroglobulin in lymph vessels of TSH stimulated guinea pigs by the indirect immunofluorescence technique.


Endocrine changes before and after the menarche. 3. Total thyroxine and "free thyroxine index", and the binding capacity of thyroxine binding proteins in female adolescents.


Peripheral plasma levels of oestrone, oestradiol-17beta and progesterone during ovulatory menstrual cycles in the rhesus monkey with special reference to the onset of menstruation.


Patterns of circulating oestrone, oestradiol-17beta and progesterone during pregnancy in the rhesus monkey.


The arterial supply to the lumbo-sacral spinal cord in the monkey: a comparison with man.


Congenital diverticulum of the left ventricle.


I segment lengths and thin filament periods in skeletal muscle fibers of the Rhesus monkey and the human.


Primary intraorbital meningiomas.

Primary intraorbital meningiomas.


Changes in blood methanol concentrations in chimpanzees during periods of chronic ethanol ingestion.


Barbiturate inhibition of growth and of synthesis of deoxyribonucleic acid, ribonucleic acid and protein in cultured mammalian cells.


Presence of histaminase and ornithine decarboxylase activities in rat thymus.


Effect of cholinesterase inhibitors on active potassium influx in monkey erythrocytes.


Malignant lymphoma with a monoclonal F(ab)mu fragment.


An alpha heavy chain abnormality in a child with hypogammaglobulinemia.


Immunobiology of human anti-IgM iso-antibodies. I. Clinical and serological studies.


Isolation and characterization of a non-specific esterase enzyme of Ascaris suum perienteric fluid.


Disc electrophoresis of Ascaris suum female reproductive system esterase enzymes.


1 Anilino-8-naphthalene sulfonate and n-phenyl-1-naphthylamine as the indicators of bacterial thermosensitivity.


Identification of a large fragment after limited cyanogen bromide cleavage of fibrinogen.


Evidence for a novel DNA component in chloroplasts of Euglena gracilis.


On the specificity of antisera against prostaglandins A2 and E2.


Variable J-chain content in human IgM.

Variable J-chain content in human IgM.


A glycosphingolipid sharing reactivity with both wheat germ lectin and "carcinoembryonic antisera (Gold)": partial identity of these reactive sites.


Analytical determination of the buoyant density of DNA in acrylamide gels after preparative CsCl gradient centrifugation.


Urinary proteins of the normal rat.

Urinary proteins of the normal rat.


Humoral and cellular immunity in the anergic tuberculosis patient. A prospective study.


Role of adipose tissue lipolysis in postnatal amelioration of thermogenesis in domestic and wild piglets.


The structural gene for the ribosomal protein S18 in Escherichia coli. II. Chemical studies on the protein S18 having an altered electrophoretic mobility.


Fine structure and life cycle of Lankesteria clarki sp. n. (Sporozoa: Eugregarinida) parasitic in the mosquito Aedes sierrensis (Ludlow).


Immunogenicity and infectivity of sporozoites of mammalian malaria isolated by density-gradient centrifugation.


DNA and RNA syntheses by intraerythrocytic stages of Plasmodium knowlesi.


Localization of some enzymes of beta-oxidation of fatty acids in the peroxisomes of Tetrahymena.


Hormonal responses to intravenous alimentation.


Filter system for intravenous alimentation.


Alteration in heterocyst pattern of Anabaena produced by 7-azatryptophan.


Slow rate of evolution of alpha A chain of alpha-crystallin.


Memory establishment via the anterior commissure of monkeys.


The presence of ribonucleotides in mature closed-circular mitochondrial DNA. Mitochondrial DNA from mouse and HeLa cells is nicked by alkali and chick-embryo ribonuclease H. It has been concluded that ribonucleotides are present in the closed-circular duplex. A comparative study of the scission rates at pH 13.0 of RNA and of mitochondrial DNAs indicates that there are no more than 10 ribonucleotides in the major fraction of the mitochondrial DNAs.


A general theory of carcinogenesis.

A general theory of carcinogenesis. A GENERAL HYPOTHESIS OF CARCINOGENESIS IS PROPOSED CONSISTING OF THE FOLLOWING FEATURES: (1) It is suggested that all cells possess multiple structural genes (Tr) capable of coding for transforming factors which can release the cell from its normal constraints on growth. (2) In adult cells they are suppressed by diploid pairs of regulatory genes and some of the transforming genes are tissue specific. (3) The Tr loci are temporarily activated at some stage of embryogenesis and possibly during some stage of the cell cycle in adult cells. (4) Spontaneous tumors, or tumors induced by chemicals or radiation, arise as the result of a double mutation of any set of regulatory genes releasing the suppression of the corresponding Tr genes and leading to transformation of the cell. (5) Autosomal dominant hereditary tumors, such as retinoblastoma, are the result of germ-line inheritance of one inactive regulatory gene. Subsequent somatic mutation of the other regulatory gene leads to tumor formation. (6) The Philadelphia chromosome produces inactivation of one regulatory gene by position effect. A somatic mutation of the other leads to chronic myelogenous leukemia. (7) Oncogenic viruses evolved by the extraction of host Tr genes with their conversion to viral transforming genes. As a result, in addition to the above mechanisms, tumors may also be produced by the reintroduction of these genes into susceptible host cells.


Production of a noncovalently bonded pentamer of immunoglobulin M: relationship of J chain. Human immunoglobulin M was reduced with concentrations of 2-mercaptoethylamine chosen so that approximately 40% of the immunoglobulin M was reduced to 7S subunits. Under these conditions, which selectively cleave intersubunit disulfides, J chain was released. The 7S subunits of immunoglobulin M so produced did not contain J chain. The high-molecular-weight immunoglobulin M remaining after treatment with 2-mercaptoethylamine had a sedimentation coefficient of 18.0 S and molecular weight of 1 million, and dissociated in 4 M guanidine into subunits similar in size to the 7S subunits. J chain was found in the 18.0S, noncovalently linked immunoglobulin M from which it was released only after more complete reduction with 10 mM dithiothreitol. The results are consistent with the hypothesis that J chain participates in the formation of the intersubunit linkages. However, it need not necessarily be directly involved in all of the intersubunit disulfides. It may play an important role in modulating the assembly of immunoglobulin M subunits, perhaps by inducing conformational changes that lead to noncovalent interactions between the subunits.


On the amino-acid sequence of flagellin from Bacillus subtilis 168: comparison with other bacterial flagellins. A partial amino-acid sequence of Bacillus subtilis 168 flagellin is presented. The region of unassigned sequence in this 304-residue polypeptide chain spans residues 158-173. Comparison of the 27-residue aminoterminal CNBr peptide of B. subtilis 168 flagellin with that derived from the flagellin of the serologically unrelated strain of B. subtilis, W23, shows only three conservative substitutions, whereas the 16-residue carboxyl-terminal peptides derived from these flagellins were identical. The comparison of the very limited sequence information available on the flagellins of Salmonella and Proteus with that on B. subtilis indicates homology between these proteins.


A wet chemical method for rendering scanning electron microscopy samples conductive and observations on the surface morphology of human erythrocytes and Ehrlich ascites cells. An alternate method to the common technique of evaporating a metallic coating on cells to render them conductive for scanning electron microscopy is described. This wet chemical technique is less expensive and easier to use, but is not as widely applicable as the evaporative technique. It should prove invaluable in the identification of artifacts by comparison of micrographs of material prepared in both manners. The first step in our application of this wet chemical method is to use the Centrifugal Cytology bucket to deposit the cells onto conductive polyethylene. After centrifugation and fixation with 4% glutaraldehyde, the sample is placed in a 50% glutaraldehyde solution. After a rinse in distilled water it is treated with ammoniacal silver nitrate. The reduced silver renders the sample conductive and, thus, for erythrocytes, eliminates artifacts due to charging and reduces these artifacts to virtually acceptable levels for larger cells. The surfaces of erythrocytes appear smooth with this technique while those coated by conventional vapor deposition of Au-Pd alloys often appear slightly wrinkled. Ehrlich ascites cells apparently can be divided into two classes by surface morphology. The surface structure of Ehrlich ascites cells rendered conductive by the wet method appears to be finer than conventionally prepared ones.


The projections of the medial geniculate complex within the sylvian fissure of the rhesus monkey.


Thrombogenic properties of heparinised vascular catheters.


The visuotopic organization of the superior colliculus of the owl monkey (Aotus trivirgatus) and the bush baby (Galago senegalensis).


Supraspinal control over segmental centers of antagonist muscles in man. 3. Tuning of spinal reciprocal inhibition system during organization preceding voluntary movement.


Changes in cerebroside synthetic activities of the brain with development of the rhesus monkey (Macaca mulatta).


Two cases of apparent exclusion of maternity caused by Rh chromosome deletion.


Planning the nutrition component of long-term care.


Effects of protein deficiency on serum albumin-synthesizing activity of rat liver--with special reference to the presence of transcriptional controls.


Immunological studies on the light chains of myosin with special reference to the relationship between light chain components.


The purification and properties of cytidine deaminase from Escherichia coli.


Further studies on a specific basic protein from bread wheat.


A study on antibodies produced with liver tissue containing Australia antigen and virus-like particles.


Experimental avian aspergillosis.

Experimental avian aspergillosis.


Rapid diagnosis of pulmonary mycosis by counterelectrophoresis.


The effect of plasma and plasma derivative infusion in the mucopolysaccharidoses.


Left ventricular volume changes after amyl nitrite and nitroglycerin in man as measured by ultrasound.


Genetic polymorphism of mannosephosphate isomerase in Primates.


Genetic polymorphism of isocitrate dehydrogenase in Primates.


Transspecific variability of carbonic anhydrase I in Primates.


Genetic polymorphism of liver esterases in Primates. I. Papio cynocephalus (Linnaeus, 1766).


Titres of Australia (hepatitis-associated) antigen in healthy carriers, leprosy, cirrhosis of the liver and acute hepatitis.


Simian model for the evaluation of immunity to influenza. Rhesus monkeys (Macaca mulatta) exposed to small-particle aerosols of the Aichi strain of type A2 influenza virus responded by shedding virus from the nasopharynx for 7 to 9 days and by seroconversion (hemagglutination inhibition) 8 or 9 days after exposure. After rechallenge with the homologous virus, no replication of the organism was observed, and a serological anamnestic reaction occurred. The data indicate that the rhesus monkey is a useful primate model for evaluating induced immunity to influenza virus infection.


Purification and characterization of Pseudomonas aeruginosa exotoxin. A trypsin-sensitive, heat-labile exotoxin of Pseudomonas aeruginosa strain P-A-103 has been purified by a procedure that includes membrane ultrafiltration, hydroxylapatite chromatography, ion-exchange cellulose chromatography, and gel filtration chromatography. The procedure resulted in the recovery of 48% of the exotoxin with a 40-fold increase in specific activity (micrograms of protein per median lethal dose [LD(50)]). The mean lethal dose of the purified toxin administered intravenously into mice weighing 20 g was approximately 6 mug of protein. The toxin contained virtually no nucleic acid, detectable pigment, or lipopolysaccharide. When subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis, the toxin separated into at least six protein components which appeared to have similar molecular weights. The estimated molecular weight of the toxin is 54,000, and its isoelectric point is 5.0


Isolation of a specific enhancer for echovirus 6 from uninfected permissive host cells. A specific enhancer for the m+ variant of echovirus 6 was isolated from uninfected, permissive host cells. The enhancer transferred the susceptibility to virus infection from permissive cells to less susceptible cells. Enhancer activity in cultured, human cells (WISH) was released by these cells into extracellular fluids at a linear rate. Maximal enhancer activity was recovered from cell monolayers that were extracted with buffered salt solutions (pH 6.6) for 5 h at 37 C. Crude enhancer preparations contained substances that reduced virus titers in permissive host cells and suppressed some of the enhancer activity. Viral inhibitory activity was removed from cellular extracts by either differential centrifugation or ion exchange chromatography. The enhancer, in contrast to inhibitory substances, remained in supernatant fractions after centrifugation at 110,000 x g for 4 h and adsorbed to diethylaminoethyl-Sephadex columns. Enhancer preparations that were devoid of inhibitory activity increased the virus titers in less susceptible simian cell cultures (approximately 100-fold) to the titers attained by fully permissive human cell cultures. A direct relationship between protein concentration and enhancer activity in cellular extracts was demonstrated. Partial purification of the enhancer by differential centrifugation and ammonium sulfate precipitation increased the specific activity of enhancer (units/mg of protein) by 13-fold.