Production of a runting syndrome and selective  A deficiency in mice by the administration of anti-heavy chain antisera. Conventional Swiss mice were treated from birth with intraperitoneal injections of anti-immunoglobulins in an attempt to create an experimental dysgammaglobulinemia. Mice treated with anti-gammaM were immunologically suppressed in all immunoglobulin classes as determined by serum antibody titers, splenic plaque-forming cells, serum immunoglobulin levels, and immunofluorescent analysis of plasma cells in lymphoid tissues. Treatment immediately after birth with high concentrations of anti-gammaM leads to a developmental arrest characterized by severe immunosuppression, failure to gain weight, and premature death. The pathogenesis of anti-gammaM runting syndrome is unknown. Animals similarly treated with anti-gammaG, anti-gammaA, or control normal goat or rabbit gamma-globulins developed normally. The frequency of occurrence and severity of anti-gammaM-induced runting syndrome is dependent upon the concentration of anti-gammaM-globulin administered. Administration of anti-gammaA resulted in a selective gammaA deficiency that was characterized by a marked reduction in serum-gammaA and an absence of gammaA-containing cells in the spleen. However, essentially normal numbers of plasma cells were found in the gastrointestinal lamina propria of anti-gammaA-treated animals concomitantly with suppressed levels of gammaA in intestinal fluids.


Neurophysiological assessment of afferent and efferent conduction in the injured spinal cord of monkeys.


Electrical properties of toad sartorius muscle fibres in summer and winter. 1. The area and circumference of surface fibres of sartorius muscles were measured from photomicrographs of frozen sections of whole muscles, and compared with the values obtained assuming a circular cross-section. The latter assumption gave an over-estimate of the mean area of 28%, but only a 2% over-estimate of the circumference. In isolated, single fibres, the assumption gave over-estimates of 25 and 6%, of area and circumference respectively.2. The passive electrical properties of fibres were different in summer and winter. The mean internal resistivity, membrane resistance and membrane capacitance were 147 Omega.cm, 7.6 kOmega.cm(2) and 4 muF/cm(2) in summer, and 194 Omega.cm, 3.9 kOmega.cm(2) and 6.7 muF/cm(2) in winter, in fibres of comparable diameters in situ. In single fibres in summer, the mean values were 120 Omega.cm, 8.6 kOmega.cm(2) and 3.6 muF/cm(2).3. In glycerol-treated fibres the mean specific membrane capacitance was 1.0 muF/cm(2) in summer and 2.0 muF/cm(2) in winter. The internal resistivity and specific membrane resistance were 167 Omega.cm and 8.9 kOmega.cm(2) in summer, and 232 Omega.cm and 3.9 kOmega.cm(2) in winter.4. Early after-depolarizations were recorded in glycerol-treated fibres which had a low membrane capacitance, did not twitch and showed little ;creep'. Electron micrographs of glycerol-treated fibres showed disruption of the transverse tubular system and sarcoplasmic reticulum.5. After exposure of muscles to 400 mM urea or acetamide for 1 hr, muscle fibres did not twitch and had a reduced membrane capacitance in Ringer solution.


Incorporation of ethanolamine into phosphatidylethanolamine in mouse liver by exchange reaction.


Progesterone-sensitive loci for blockade of ovulation in the hamster. Virgin female golden hamsters, 3-6 months old were implanted with progesterone, testosterone or cholesterol fused within the lumen of a hypodermic needle, or progesterone, cholesterol or paraffin fused into the end of a hypodermic tube or  tungsten wire in order to determine progesterone-sensitive loci for blocking of ovulation.  Molten steroid was drawn into 22-guage "thin wall", 27-guage 30-guage hypodermic tubing and then solidified.  Large pellets (450 mcm) were made by dipping a 30-guage tube into molten steroid.  Smaller pellets were made on the ends of tungsten wire (130 mcm).  Pellets were measured on a shadowgraph to the nearest 10 mcm.  The pellets were implanted through the top of the cranium and fastened with Kadon 22 dental cement.  The implantation of 22-guage "thin wall" implants was performed 52-56 hours before ovulation and other implants 32-39 hours before the next expected ovulation.  Hamsters were checked daily for ovulation by method of Orsini for 2 weeks after implantation.  A hemi-ovarectomy and search for ova according to Reuter et al was performed on the day of their expected ovulation.  The ovary was dissected and examined (10-30X magnification).  The animals were sacrificed and brains removed, fixed in 10% formalin, serially sectioned at 100 mcm using a clinical freezing microtome, and stained with thronine to determine implantation site.  Progesterone implants 18,000 sq. mcm larger resulted in blocking ovulation in the medial preoptic-diagonal band region of the hypothalamus.  28,000- 180,000 sq. mcm sizes also blocked ovulation when placed in the median eminence and in acurate nucleus regions of the hypothalamus but not in the hypophysis.  The blocking effect of the hypophyseal implants can be explained by the lesion caused by the physical size of implants.  Control substances did not block ovulation except when related to lesions caused by 450 mcm or 710 mcm diameter structures.  Implants were effective in blocking ovulation for 2 weeks in larger implants and 1-3 days for smaller ones.  The reason for this was not understood.  Testosterone was also able to block ovulation in the medial preoptic-diagonal band region but to a significantly lesser degree than progesterone.


Oocysts of Isospora papionis in the skeletal muscles of chacma baboons.


The experimental transmission of Theileria ovis by Rhipicephalus evertsi mimeticus and R. bursa.


Some protozoan parasites of tragelaphine antelopes in South Africa with special reference to a Babesia sp. in a bushbuck and a Trypanosoma Theileri-like parasite in a Nyala.


Mycobacteria of surgical interest.

Mycobacteria of surgical interest.


The present status of one-stage pharyngo-esophageal diverticulectomy.


Tyrosinase in melanized melanosomes.

Tyrosinase in melanized melanosomes.


The value of electrodiagnostic tests in pigmentary retinopathy.


Immunological responsiveness and tolerance of marmoset lymphoid tissue in vitro.


Modification of hyperacute xenograft rejection by intra-arterial infusion of disodium ethylenediaminetetraacetate.


Disturbances of coagulation mechanism in protein-calorie malnutrition.


Comparative histoenzymatic studies on the distribution of alkaline and acid phosphatases in the liver and pancreas of a few birds viz., Upupa epops (fam. Upupidae), Cinnyris asiatica (fam. Nectariniidae) and Halcyon smyrnensis (fam. Alcedinidae).


Lysozyme in Paneth cell secretions.

Lysozyme in Paneth cell secretions.


Linear dichroism and anomalous dispersion of the birefringence on sperm heads.


Hypokinetic effects on the RNA and protein metabolism in motoneurons of anterior horns of the rat spinal cord.


Polarographic investigations on Pb ++  trapping by rat liver homogenate.


Differentiation of noradrenaline and adrenaline containing cells in the adrenal medulla by the trihydroxyindole histochemical reaction.


Testicular leucine aminopeptidase in different vertebrates.


Effects of alprenolol and isosorbide dinitrate in angina pectoris. A comparative study with methodological considerations.


A new hemodialysis console. I. General description.


Changes in antithrombin 3 levels following cessation of anticoagulant therapy.


Alprenolol alone and in conjunction with pentanitrol in angina pectoris. A double-blind study with exercise tests.


Experimental zinc chloride ocular injury and treatment with disodium edetate.


Immunoglobulins in tears.

Immunoglobulins in tears.


Recovery of patients after intensive care for blunt injuries.


Biliary excretion of  14 C-edrophonium and its glucuronide metabolite in Gunn rats.


Interaction between drugs using three-dimensional isobolographic interpretation.


Vascular reactions in the musculo-cutaneous area during the hypertension induced by acetylcholine in atropinized dogs.


Pharmacological investigations with benzoctamine (Tacitin), a new psycho-active agent.


The persistence of courtship stimulation in Drosophila melanogaster.


Simple methods for plating single vegetative cells of, and for replica-plating, filamentous blue-green algae.


Regulation of gene function: a comparison of enzyme activity levels in relation to gene dosage in diploids and triploids of Drosophila melanogaster.


Alternative interpretation of the molecular structure and somatic genetics of acid phosphatase-1 in Tetrahymena pyriformis.


IgA-induced anaphylactic transfusion reactions: a report of four cases.


Properties of salivary basic proteins showing polymorphism.


Analgetic activities of etoxadrol in the rhesus monkey and in mice.


The invasion of burned skin by Pseudomonas aeruginosa.


Haemophilus influenzae cellulitis. Case presentation and review of the literature.


Therapeutics XXII. Drug interaction. I.

Therapeutics XXII. Drug interaction. I.


Immune globulin characterization of complement fixing antibodies in histoplasmic infection.


Left ventricular angiography as a function test.


The Ig class and light chain type of the long acting thyroid stimulator.


Human chorionic somatomammotrophin and its clinical significance.


Incorporation of  32 P during early amphibian embryogenesis.


Combinations of the effect of radiotherapy, alkylating agents and phytohemagglutinin on sarcoma 180 in Swiss mice.


Characteristics of the reverse passive respiratory reaction in Rhesus monkeys.


The biochemical properties of esterases in Cepaea (Mollusca: Helicidae).


Characterization of an Escherichia coli mutant deficient in dihydrolipoyl dehydrogenase activity. A mutant of Escherichia coli deficient in dihydrolipoyl dehydrogenase (DHL) activity has been isolated and its characteristics have been studied. The activities of the pyruvic dehydrogenase (PDC) and alpha-ketoglutaric dehydrogenase complexes (KDC) are not present in extracts of the mutant unless purified dihydrolipoyl dehydrogenase is added. Experiments with antiserum to DHL have shown that cross-reacting material exists in mutant extracts. This suggests that the dihydrolipoyl dehydrogenase mutation (dhl(-)) is a missense structural mutation. The mutation maps very close to, if not adjacent to, the ace loci, and is not linked to the suc loci. This means the dhl locus is grouped with the genes for the other components of the PDC and not with the genes for KDC. The mutation is also transducible into prototrophic strains, demonstrating that no prior mutation is necessary for the DHL activity deficiency to exist. This evidence is consistent with the idea that there is only one gene for DHL and is supported by previous biochemical studies which have shown that DHL preparations from either enzyme complex are electrophoretically and immunochemically indistinguishable. Possible mechanisms for the genetic and metabolic control of DHL, PDC, and KDC are discussed.


A bifunctional enzyme in Pseudomonas aeruginosa: a new pattern in the organization of enzymes concerned with phenylalanine and tyrosine biosynthesis. Two isozymes of chorismate mutase (CA mutase(1) and CA mutase(2)) and two isozymes of prephenate dehydratase (PPA dehydratase(1) and PPA dehydratase(2)) have been found in Pseudomonas aeruginosa. The activities CA mutase(2)-PPA dehydratase(2) catalyzing phenylalanine biosynthesis have been purified almost 40-fold and were found to be associated as a bifunctional enzyme or an enzyme complex. The enzymes specific for tyrosine biosynthesis did not appear to manifest such physical association. Thus, the organization of enzymes concerned with phenylalanine and tyrosine biosynthesis in P. aeruginosa is unique and is unlike most other organisms. Single site mutants have been isolated which have lost both CA mutase(2)-PPA dehydratase(2) activities resulting in a requirement for phenylalanine for growth. Single site revertants of these mutants regained both these activities simultaneously and were able to grow on minimal medium. A mutant, r(6), was also isolated which had normal CA mutase(2) but lacked PPA dehydratase(2) activity.


Chemical analysis of cell walls and autolytic digests of Bacillus psychrophilus. The amino acid composition of isolated cell walls of Bacillus psychrophilus has been determined before and after extraction of protein with ethylenediamine-tetraacetic acid at 45 C. This revealed that the peptidoglycan consists of Ala, Lys, and Glu in a molar ratio of 3:1:2. By using autolytic digests of log-phase cell walls, it was possible to detect 14 ninhydrin-positive degradation products. Chemical analyses of the seven major bands from these digests indicated that the amino acid sequence of the peptide subunit in the murein of this organism consists of muramyl-l-alanyl-gamma-d-glutamyl-l-lysyl- d-alanine, and the linkage between adjacent peptides is supplied by a second d-glutamic acid which is bound to the sigma-amino group of lysine and the carboxyl group of the d-alanine through its amino group. The nature of the solubilized wall fragments indicates that each of the peptide bonds in the murein is hydrolyzed by autolysins except the l-alanyl-gamma-d-glutamyl linkage.


A growth initiation factor for host-independent derivatives of Bdellovibrio bacteriovorus. Host-independent (H-I) derivatives of Bdellovibrio bacteriovorus 109 Davis could not be isolated when concentrated suspensions of host-dependent (H-D) cultures, washed free of spent medium, were plated on host-free media. However, H-I colonies did appear when spent broth was incorporated into the isolation medium, indicating the presence of a factor in the spent medium essential for the growth of H-I cells. This growth factor (GIF) was also present in cell-free extracts of Escherichia coli and a variety of other microorganisms including H-D and H-I derivatives of strain 109 Davis. GIF was heat stable, non-dialyzable, and present in both soluble and particulate fractions of extracts. Heating of extracts at 70 C for 10 min resulted in 10- to 40-fold stimulation in GIF activity, and evidence for a heat-labile inhibitor was obtained. Colonies appearing on host-free medium in these experiments were shown to be those of typical H-I derivatives by isolation and subsequent host-independent cultivation of these organisms. GIF was a conditional requirement dependent on age and size of inoculum for all H-I derivatives characterized. Although GIF stimulated the growth of washed exponential phase cells transferred to fresh medium, it was not essential for growth. However, it was essential for the initiation of growth of washed stationary phase cells from small inocula transferred to fresh medium. It is proposed that GIF is required to initiate growth of metabolically quiescent cells.


Fatty acids of Myxococcus xanthus.

Fatty acids of Myxococcus xanthus. Fatty acids were extracted from saponified vegetative cells and myxospores of Myxococcus xanthus and examined as the methyl esters by gas-liquid chromatography. The acids consisted mainly of C(14) to C(17) species. Branched acids predominated, and iso-pentadecanoic acid constituted half or more of the mixture. The other leading component (11-28%) was found to be 11-n-hexadecenoic acid. Among the unsaturated acids were two diunsaturated ones, an n-hexadecadienoic acid and an iso-heptadecadienoic acid. No significant differences between the fatty acid compositions of the vegetative cells and myxospores could be detected. The fatty acid composition of M. xanthus was found to be markedly similar to that of Stigmatella aurantiaca. It is suggested that a fatty acid pattern consisting of a large proportion of iso-branched C(15) and C(17) acids and a substantial amount of an n-16:1 acid is characteristic of myxobacteria.


Biological activity of heated diphtheria toxin. Diphtheria toxin splits into two fragments when heated at 100 C for 10 min in a phosphate buffer. The separated fragments have molecular weights of 24,000 and 39,000, respectively. These molecular weights are similar to those of the A and B fragments found in diphtheria toxin preparations after thiol reduction. Since the separation of toxin into fragments is not complete, it is likely that only nicked toxin molecules having a cleaved peptide bond are split by heating. When toxin is suspended in phosphate buffer at pH 6.4, the B-like fragment precipitates, but at pH 7.8 it does not. Heated toxin is unable to intoxicate sensitive cells or cause a necrodermal response in animals. Fragment A produced by heating is active in inhibiting cell-free protein synthesis. It is able to intoxicate both HeLa and L cells when the uptake of the fragment is facilitated by addition of polyornithine to the cultures. Fragment B produced by heating is involved with binding to the cell surface. It is able to delay the action of toxin on KB cell cultures preincubated with fragment B.


Scanning electron microscopy of colonies of six species of Candida. Thirty strains of six species of Candida isolated from patients were cultured for 60 h on Sabouraud agar, freeze-dried, and examined with a scanning electron microscope. The colonies were circular (Candida albicans, C. guilliermondii) or oval (C. tropicalis, C. pseudotropicalis, C. krusei, C. parakrusei) in outline, and those of C. pseudotropicalis and C. krusei had an irregular outline due to a peripheral pseudomycelium. The morphology of individual microorganisms was examined at the margins and apex of those species which lacked a surface coat (C. pseudotropicalis, C. krusei, C. parakrusei, C. guilliermondii), and through cracks in the surface coating of those which showed a surface coat (C. albicans, C. tropicalis). All species showed buds, bud scars, and interconnecting intercellular processes, but were generally spherical (C. albicans, C. tropicalis) or ovoid (C. pseudotropicalis, C. krusei, C. parakrusei, C. guilliermondii) in fixed preparations. In unfixed material, individual organisms were almost invariably indented. Fixation with 3% glutaraldehyde and washing before freeze-drying caused partial removal of the surface coating of colonies of C. albicans and C. tropicalis, which persisted only as irregular sheets or as a filamentous meshwork. This filamentous meshwork was also present among the organisms of colonies of C. albicans, C. tropicalis, and C. pseudotropicalis. It is concluded that these filaments represent the precipitation or unmasking of some component of the intercellular matrix of these organisms.


Cell surface protein of Pseudomonas (Hydrogenomonas) facilis. Intact cells of Pseudomonas facilis contain one major molecular weight class of protein that is exposed at the cell surface as revealed by lactoperoxidase-catalyzed iodination with (125)I. All molecular weight classes of protein in derived cell envelope preparations are apparently saturated by iodination by lactoperoxidase after prolonged sonic treatment. The molecular weight of the predominantly exposed protein in intact cells is approximately 16,000, which is the minimal molecular weight of a cell envelope protein that precipitates as a complex with phospholipid from extracts of P. facilis. The isolation of labeled phospholipoprotein (PLP) after labeling intact cells with (125)I corroborates previous experiments which suggested a surface location for the protein portion of the phospholipoprotein (P(PLP)). Solvent extraction of cells and immunological evidence, including studies with ferritin-coupled antibodies, indicate that P(PLP) is located at the cell surface and may also be within the cell envelope. These experiments suggest that P(PLP) is the major cell surface protein in P. facilis.


Purification of Synechococcus lividus by equilibrium centrifugation and its synchronization by differential centrifugation. A culture of the thermophilic, unicellular, blue-green alga Synechococcus lividus was freed from two thermophilic bacteria by use of equilibrium (isopycnic) centrifugation in Ficoll density gradients. After removal of the bacteria, the alga would grow only on agar plates in a high carbon dioxide atmosphere. Intermittent illumination, equilibrium centrifugation, and differential centrifugation were tested as techniques for obtaining synchronized cultures of the alga. Daughter cells selected by differential centrifugation in Ficoll density gradients yielded the best synchrony, and a cross-wall index of 85% was observed during the period of cell division.


Loss of photosynthetic activity in two blue-green algae as a result of osmotic stress. A progressive loss of photosynthetic activity occurred when cells of Anacystis nidulans and Coccochloris peniocystis were incubated in increasing concentrations of osmotica from 0.2 to 0.7 M, and there was a release of pteridine from the cells proportional to the loss of photosynthetic activity.


Agglutination of bacterial spheroplasts: agglutination-dependent degradation of Escherichia coli ribosomal ribonucleic acid. When Escherichia coli spheroplasts made by ethylenediaminetetraacetic acid and lysozyme were agglutinated by concanavalin A (con A), the degradation of ribosomal ribonucleic acid (rRNA) was found to occur proportionally to the degree of the agglutination, which was determined by microscopic examination or by a newly devised assay based on the slower settling of aggregates. Methyl-alpha-d-glucoside, low temperature or alkaline pH, all of which reverse the agglutination, also reduced the extent of rRNA degradation. This degradation was not due to the direct action of con A since a similar relationship was found in the case of spontaneous agglutination with concentrated spheroplasts in the absence of con A. The possible importance of a change in the cell membrane associated with the agglutination process is discussed in connection with the initiation of rRNA degradation.


Fatty acid composition of L-forms of Streptococcus faecalis cultured at different osmolalities. The fatty acid composition of the membranes of three different penicillin-produced L-forms of Streptococcus faecalis was determined: (i) a stable (nonreverting) L-form (T(53)) cultured in brain heart infusion (BHI) with 0.5 M sucrose; (ii) a stable L-form (T(531)) cultured in BHI without sucrose; and (iii) an unstable L-form (T(9)) cultured in BHI with 0.5 M sucrose and 1,000 U of penicillin per ml. L-forms were obtained by centrifugation and lysed by washing in 1 mM tris(hydroxymethyl)aminomethane-hydrochloride buffer. The parent S. faecalis was also cultured in BHI and BHI containing 0.5 M sucrose, and washed with buffer. The fatty acid composition of L-forms of S. faecalis cultured in BHI without sucrose (370 mosmol) had higher C(18:1) and lower C(18) than L-forms cultured in the same media with added 0.5 M sucrose (950 mosmol) in both exponential and stationary cultures. In the stationary phase of growth, C(19) was reduced in the L-forms cultured without sucrose. Similar changes were seen in the parent S. faecalis cultured in the two types of media. These changes in membrane fatty acids may relate to osmo-regulation of the L-forms.


Dissociated learning and state-dependent retention induced by pentobarbital in rhesus monkeys.


Detergent-dispersed adenylate cyclase from rat brain. Effects of fluoride, cations, and chelators.


Glucosamine HCl, hydroxyproline and developmental homoeostasis in Drosophila melanogaster.


H. influenzae pneumonia in infants and children.


Insulin and sodium bicarbonate treatment of diabetic acidosis: a retrospective review.


Cromolyn therapy for severe asthma in children.


Blood lipid alterations in infants receiving intravenous fat-free alimentation.


Postexchange transfusion hepatitis in the newborn infant.


Physiotherapy and the care of the progressively ill patient. 2. The unconscious and bed-ridden patient.


Serological titres following vaccination of sheep and goats with Brucella melitensis rev I vaccine.


A serological investigation on adult cattle vaccinated with Brucella abortus strain 19.


Prostaglandins and food intake of rats: a component of energy balance regulation?


Hyperphagia induced by intrahypothalamic implants of mercury thioglucose.


Diabetes and pregnancy.

Diabetes and pregnancy.


A simple method for measuring spontaneous motor activity in squirrel monkeys during chair restraint.


Neurophysiology of flight in wild-type and a mutant Drosophila. We report the flight motor output pattern in Drosophila melanogaster and the neural network responsible for it, and describe the bursting motor output pattern in a mutant. There are 26 singly-innervated muscle fibers. There are two basic firing patterns: phase progression, shown by units that receive a common input but have no cross-connections, and phase stability, in which synergic units, receiving a common input and inhibiting each other, fire in a repeating sequence. Flies carrying the mutation stripe cannot fly. Their motor output is reduced to a short duration, high-frequency burst, but the patterning within bursts shows many of the characteristics of the wild type. The mutation is restricted in its effect, as the nervous system has normal morphology by light microscopy and other behaviors of the mutant are normal.


Effect of hemin on the synthesis of hemoglobin and other proteins in mammalian cells. The initiation of globin synthesis in intact reticulocytes and in reticulocyte lysates is maintained by the addition of hemin. The specificity of this effect has been studied to determine whether it is restricted to hemoglobin and erythroid cells. In intact reticulocytes, hemin (500 muM) enhances the synthesis of carbonic anhydrase as well as of hemoglobin. Similar enhancement of protein synthesis is observed on addition of hemin (500 muM) to intact Krebs II ascites tumor cells, in cell-free extracts of these cells, added hemin (50 muM) increases endogenous protein synthesis and the translation of exogenous rabbit globin messenger RNA. These results provide evidence that the effect of hemin is not restricted to globin, and they suggest that hemin may enhance protein synthesis in at least some nonerythroid cells.


Mechanism of action of 1- -D-ribofuranosyl-1,2,4-triazole-3-carboxamide (Virazole), a new broad-spectrum antiviral agent. The antiviral activity of the synthetic nucleoside, Virazole (1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide), against measles virus in Vero cell cultures was substantially reversed by xanthosine, guanosine, and to a slightly lesser extent by inosine. Virazole 5'-phosphate was subsequently found to be a potent competitive inhibitor of inosine 5'-phosphate dehydrogenase (IMP:NAD(+) oxidoreductase, EC 1.2.1.14) isolated from Escherichia coli (K(m) = 1.8 x 10(-5) M) with a K(i) of 2.7 x 10(-7) M. Guanosine 5'-phosphate (GMP) was a competitive inhibitor of this enzyme with a K(i) of 7.7 x 10(-5) M. Virazole 5'-phosphate was similarly active against IMP dehydrogenase isolated from Ehrlich ascites tumor cells, with a K(i) of 2.5 x 10(-7) M. The K(m) for this enzyme was 1.8 x 10(-5) M, and the K(i) for GMP was 2.2 x 10(-4) M. These results suggest that the antiviral activity of Virazole might be due to the inhibition of GMP biosynthesis in the infected cell at the step involving the conversion of IMP to xanthosine 5'-phosphate. This inhibition would consequently result in inhibition of the synthesis of vital viral nucleic acid.


An RNA polymerase mutation causing temperature-sensitive sporulation in Bacillus subtilis. A single-site mutant of Bacillus subtilis with a rifampin-resistant RNA polymerase has been isolated; this mutation causes temperature-sensitive sporulation. The temperature-sensitive mutation was only expressed during a limited time period, covering the middle third of the sporulation sequence. Mutant cells grown at the nonpermissive temperature exhibited the normal change in RNA polymerase template specificity, accumulated extracellular proteolytic activity and antibiotic activity, but failed to accumulate alkaline phosphatase and, hence, were blocked at or near stage III in the sporulation sequence. Pulse-labeled RNA synthesis was seriously deranged during postexponential growth phase in mutant cells incubated at the nonpermissive temperature.


Magnetic dipole-dipole coupled Cu(II) pairs in nitric oxide-treated tyrosinase: a structural relationship between the active sites of tyrosinase and hemocyanin. The T(r) and T[unk] states of tyrosinase were treated with NO. EPR spectra of the products observed at 14 degrees K and at 113 degrees K showed mixtures of two signals. One had components in the region of g = 2, about 1200 G wide, and in the region of g = 4, showing hyperfine splitting. The other signal was similar to that arising from isolated Cu(II) ions in an axially symmetric environment. The first signal was indicative of Deltam = 1 and Deltam = 2 transitions arising from magnetic dipole-dipole coupled Cu(II) ion pairs. It closely resembled previously reported EPR spectra obtained from NO-treated hemocyanin, which were confirmed in this study. The normal Curie behavior of the signals between 230 degrees K and 14 degrees K ruled out significant exchange coupling between the ion pairs. The Deltam = 2 signals were not saturable up to 350 mW at 14 degrees K. The broad Deltam = 1 signals could be separated from accompanying signals by the saturation characteristics of the latter at about 10 mW at 14 degrees K. The results establish the presence of a pair of copper ions at the active site of tyrosinase, and a clsoe structural relationship between this active site and that of hemocyanin.


Inhibition of cellular protein synthesis by double-stranded RNA: inactivation of an initiation factor. Inhibition of protein synthesis in rabbit reticulocyte lysates by double-stranded RNA is caused by the inactivation of IF-3, an initiation factor required for the recycling of ribosomes and for their binding to messenger RNA. The evidence for this is that (i) the inhibition can be overcome by addition of exogenous If-3; (ii) double-stranded RNA inactivates stoichiometric amounts of IF-3; (iii) double-stranded RNA forms a complex with IF-3; and (iv) double-stranded DNA, which lacks inhibitory activity, also binds to IF-3, but with a much lower affinity than double-stranded RNA. It is concluded that double-stranded RNA inhibits cellular protein synthesis by tightly complexing with IF-3. It is suggested that IF-3 normally recognizes a double-stranded region in messenger RNA.


Epilepsy after non-missile head injuries.


Stroma-free hemoglobin. I. Preparation and observations on in vitro changes in coagulation.


Altered insulin and glucose metabolism produced by epinephrine during hemorrhagic shock in the adrenalectomized primate.


The effect of hemorrhagic shock on intracellular muscle action potentials in the primate.


A brief note on the chemical nature of the precipitate within nerve fibers after the rapid Golgi reaction: selected area diffraction in high voltage electron microscopy.


Architectonic parcellation of the temporal operculum in rhesus monkey and its projection pattern.


-aminolaevulinic acid synthetase activity in normal human liver homogenate.


Factors influencing splenic pooling of erythrocytes in the myelo- and lympho-proliferative syndromes.


Serum B 12  binders in vitamin B 12  deficiency. Relation of serum B 12  and haemoglobin levels with B 12  binders.


Effect of corticosteroids on eosinophil leukocytes in hypereosinophilic syndromes.


Haematological effects of chronic ethanol administration and folic acid deficiency in mice.


Adult human adipose tissue cellularity and metabolism with special reference to obesity and fatty acid synthesis de novo.


Calciun-sensitive site in the carotid trunk at the thyroid artery issue, influencing the respiratory centre.


Tumor-like lesion of the brain caused by Trypanosoma cruzi.


Some epidemiological considerations of scrub typhus (Rickettsia tsutsugamushi) in a natural focus in the Zambales Mountains, Luzon, Republic of the Philippines.


Annually recurrent epidemic polyarthritis and Ross river virus activity in a coastal area of new New South Wales. I. Occurrence of the disease.


Anticonvulsant drugs in human epileptogenic brain. Correlation of phenobarbital and diphenylhydantoin levels with plasma.


Systemic factors and epileptic brain damage. Prolonged seizures in paralyzed, artificially ventilated baboons.


Size dependent separation of high molecular weight double-stranded DNA by means of gel electrophoresis.


Dynamic aspects of membrane-bound DNA in Bacillus subtilis during the course of synchronous growth.


Amino acid sequence of human erythrocyte carbonic anhydrase C.


Induction of biohydrogenation of oleic acid in Bacillus cereus by increase in temperature.


Energy-linked swelling of EDTA submitochondrial particles.


Molecular organization in bacterial cell membranes. II. Reevaluation and identification of some chemical components of Micrococcus lysodeikticus membranes.


Euglycemic diabetic ketoacidosis.

Euglycemic diabetic ketoacidosis.


Monkeys for research.

Monkeys for research.


Morphological observations on the contact-induced lysis of target cells.


Macrophage cytotoxicity factor. A product of in vitro sensitized thymus-dependent cells.


Circulating immune complexes in inflammatory bowel disease.


Rosette formation by human T and B lymphocytes.


Plasmodium berghei: folic acid levels in mouse erythrocytes.


Plasmodium falciparum and P. knowlesi: low temperature preservation using dimethylsulfoxide.


Studies in myasthenia gravis. Heat stability and species specificity of active muscle antigens.


Scoliosis and neurological disease.

Scoliosis and neurological disease.


Role of anaerobic coryneforms in specific and non-specific immunological reactions. II. Production of a chemotactic factor specific for macrophages.


A direct turbidimetric assay of pyocine.


Cross reactions between the antigen of human lymphatic tissues and antigens of monkey, cow, mouse and hamster tissues.


Some aspects of the mode of action of the antibacterial compound bronopol (2-bromo-2-nitropropan-1,3-diol).


The influence of sporulation and germination media on the development of spores of Bacillus megaterium and their inhibition by chlorocresol.


Immunochemical analysis of chick phosvitin.


Pulmonary host defenses. I. Analysis of protein and lipids in bronchial secretions and antibody responses after vaccination with pseudomonas aeruginosa.


Protection against enteric bacterial infection by secretory IgA antibodies.


Pulmonary host defenses. II. Interaction of respiratory antibodies with Pseudomonas aeruginosa and alveolar macrophages.


Immunologic studies on drug addiction. I. Antibodies reactive with methadone and their use for detection of the drug.


A latex agglutination test for measuring antibodies to Streptococcal mucopeptides.


Application of electroimmunodiffusion and crossed electroimmunodiffusion to the comparative serology of a microorganism (Histoplasma capsulatum).


Immunogenicity and arthritogenicity in the rat of an antigen from Mycobacterium tuberculosis wax D.


Radioimmunologic measurements of naturally occurring bacterial antibodies. I. Human serum antibodies reactive with Escherichia coli in gastrointestinal and immunologic disorders.


IgG, IgA, and IgM cold-reactive immunoglobulins in 19 patients with elevated cold agglutinins.


Lymphocyte function in normal people with persistent Australia antigen.


Measurement of the third component of complement in cerebrospinal fluid by modified electroimmunodiffusion.


Isolated in vivo perfusion of the baboon brain with uncomplicated survival: a new experimental model.


Cisternography: from early tribulations to a useful diagnostic procedure.


An automated method for microbiological assays.


Observations on Listeria monocytogenes type 5 (Iwanow) isolated in New Zealand.


Three new fragments, F(ab) 2 , F(c) 2 , and Fab-c, obtained by papain proteolysis of normal human IgG.


Lectins in precipitin reactions with soluble H substance of human saliva and serum. Lectins that precipitate the soluble H substances of saliva were produced for the first time from the four commonly used sources of seed. In Ouchterlony precipitin reactions, identity was observed between extracts of Cytisus sessilifolius and Laburnum alpinum, but these extracts displayed incomplete identity with extracts from Ulex europaeus. These three sources of the precipitating lectin formed bands when tested with saliva from all secretors and none with saliva from nonsecretors. Extracts from Lotus tetragonolobus, however, formed two bands with saliva from secretors and one strong band with all saliva from nonsecretors. This new antigen, which reacts with Lotus, is present in all saliva and on red cells of all normal individuals, but it is absent in the O(h) (Bombay) type. The lectins formed bands with serums of secretor and nonsecretor individuals, but these bands were weaker than those with saliva, and revealed incomplete identity between H substance of serum and saliva. The precipitin reaction of the lectins is an efficient method for demonstrating relations among lectins and among antigens and has led to the discovery of a new human antigen.


The picornaviruses of epidemic conjunctivitis.


Coagulation and fibrinolysis in simian Plasmodium knowlesi malaria.


Studies on the phagocytic activity of the reticuloendothelial system using  131 I-AA in normal monkeys.


The mode of action of Sterinol on microorganisms: effect on the external layers of bacterial cells.


The morphology of the juxtaglomerular apparatus in the toad, Bufo bufo. A light microscopic study.


Perforation of a jejunal diverticulum containing a lymphosarcoma and accompanied by upper gastrointestinal hemorrhage. Report of a case.


Early stages of synaptogenesis in the cervical spinal cord of the chick embryo.


A survey of 99 surgical patients treated with gentamycin.


Foamy virus serotypes 1 and 2 in rhesus monkey tissues.


Bovine ephemeral fever virus in cell culture and mice.


Factors affecting the response of microsomal UDP-glucuronyltransferase to membrane perturbants.


Gold nephropathy. An immunopathologic study.


On the nature of the mid-oseophageal diverticulum.


Characteristics of H +  and NH 4  +  excretion by the urinary bladder of the toad.


Non-autonomy of hormone secretion in primary hyperparathyroidism.


Neoplastic changes in the rat liver induced by polychlorinated biphenyl.


Carriers of hepatitis B antigen: an epidemiologic and histologic study.


Evaluation of the vaginal tampon as a means of obtaining cultures for Neisseria gonorrhoeae.


Studies on the pathogenesis of dengue infection in monkeys. II. Clinical laboratory responses to heterologous infection.


Studies on the pathogenesis of dengue infection in monkeys. 3. Sequential distribution of virus in primary and heterologous infections.


Antigenicity of a polysaccharide vaccine from Neisseria meningitidis administered intranasally.


Studies on the pathogenesis of dengue infection in monkeys. I. Clinical laboratory responses to primary infection.


Potent anti-inflammatory N-heterocyclic 3-carboxamides of 4-hydroxy-2-methyl-2H-1,2-benzothiazine 1,1-dioxide.


Nitrones. 6.  -(5-Nitroimidazol-2-yl)-N-substituted nitrones.


Selection of a fast-growing Chikungunya virus by passage from suckling mouse brain to VERO cells.


Contamination of methylmethacrylate bone cements.


The use of a balloon catheter in the induction of labour.


Diseases of the alimentary tract and western diets.


Oculomotor deficiency after cerebellar cortical lesions.


Toxicogenic potentials of biomaterials and methods for evaluating toxicity.


Structure and connections of the rhesus vestibular cortex.


Ultrastructure of the otolithic membrane and the cupula. A scanning electron microscopic observation.


Prebiotic evolution of enzyme-like systems.


Netherlands Society for Microbiology meeting at Delft on 25 October 1972. Mycotoxins.


The ultrastructure of the blood vessels of the human dental pulp following injury. IV.


Serum immunoglobulin conten in normal and lymphoid tumor-bearing Xenopus laevis.


The frequency of other handicaps in children with cerebral palsy.


The influence of antibovine alpha-crystallin on the development of the rabbit eye.


Integumental distribution of tyrosinase activity in the slow loris, Nycticebus coucang, and the presence of integumental tyrosinase inhibitor(s).


The topography of the premaxillary-frontal region in nonhuman primates.


Asymmetrical responses to direction selection for radiation resistance and sensitivity in Drosophila.


Chromosomal evolution in a rhesus cell line.


Social behavior comparison in laboratory-reared stumptail and rhesus macaques.


An X-O anomaly and ovarian dysgenesis in a rhesus monkey.


The reproductive cycles of two subspecies of purple-faced langurs (Presbytic senex) with relation to environmental factors.


Homosexual behavior and dominance hierarchy in a group of captive female squirrel monkeys (Saimiri sciureus).


Chloroplast membrane proteins. II. Solubilization of the lipophilic components.


Subunit structure of fragment D from fibrinogen and cross-linked fibrin.


Polymorphism of rhesus monkey serum prealbumin. Molecular properties and binding of thyroxine and retinol-binding protein.


Mammary tumor regression. V. Role of acid ribonuclease and cathepsin.


Mammary tumor regression. VI. Synthesis and degradation of acid ribonuclease.


Purification and comparison of sex-dependent and sex-independent gulonolactonase of rats.


The relationship of serine protease activity to RNA polymerase modification and sporulation in Bacillus subtilis.


Basal study on early diagnosis of cadmium poisoning: change in carbonic anhydrase activity.


Serum immunoglobulins and coproantibody formation in infants after artificial intestinal colonization with Escherichia coli 083 and oral lysozyme administration.


The biology of human aging.

The biology of human aging.


Studies on human thyroxine-binding globulin. 8. Isoelectric focusing evidence for microheterogeneity of thyroxine-binding globulin.


Purification and properties of rat adrenal phenylethanolamine-N-methyl transferase.


Studies on the immunological cross-reactivity and physical properties of fatty acid synthetases.


Effect of secondary interaction on the enzymatic activity of trypsin-like enzymes from Streptomyces.


Intestinal malabsorption in malnourished children and during recovery. Duodenal contents of lipase, nitrogen, and micellar fat after fat stimulation.


Solitary mastocytoma.

Solitary mastocytoma.


The bacterial degradation of flavonoids. Oxidative fission of the A-ring of dihydrogossypetin by a Pseudomonas sp. Cell-free extracts prepared from a Pseudomonas sp., grown on (+)-catechin, oxidized dihydrogossypetin (3',4',5,7,8-pentahydroxyflavanonol) by cleaving the A-ring to form oxaloacetic acid from C-5, C-6, C-7 and C-8 together with 5-(3,4-dihydroxyphenyl)-4-hydroxy-3-oxovalero-delta-lactone. The structure of this lactone was confirmed by synthesis of related phenylvalerolactones.


Stimulatin of tryptophan metabolism in the rat by 4-dimethylamino-3'methylazobenzene and 2-acetamidofluorene.


Sporulation in Bacillus subtilis. Properties and time of synthesis of alkali-soluble protein of the spore coat. An alkali-extractable protein fraction comprising 6% of the dry weight of the spore can be removed from spores of Bacillus subtilis 168. Three different extraction procedures each yield at least one similar protein. Extracted protein behaved as a single species on ion-exchange chromatography or gel filtration, but two polypeptides were found on electrophoresis. Comparison of molecular weights on electrophoresis and by sucrosegradient analysis suggests that the protein(s) undergo aggregation into multimers. Extracted spores remain viable, but are altered in density and lysozyme sensitivity and they aggregate together. Electron microscopy of extracted spores shows that loss of material seems to occur from the outer coat layers. Extraction therefore probably removes a specific fraction of the spore-coat protein, but without impairment to the spore protoplast. This protein can first be detected immunologically 4h after the initiation of sporulation, and the synthesis of this protein is sensitive to chloramphenicol, actinomycin D and rifamycin. Labelling experiments also show that the protein begins to be synthesized early in sporulation. Examination of the ability of asporogenous mutants to produce cross-reacting material indicates that some event in stage II of sporulation determines its production.


The uptake and metabolism of plasma lysophosphatidylcholine in vivo by the brain of squirrel monkeys. 1. Adult squirrel monkeys were injected intravenously with doubly labelled lysophosphatidylcholine (a mixture of 1-[1-(14)C]palmitoyl-sn-glycero-3-phosphorylcholine and 1-acyl-sn-glycero-3-phosphoryl[Me-(3)H]choline; (3)H:(14)Cratio 3.75) complexed to albumin, and the incorporation into the brain was studied at times up to 3h. 2. After 20min, 1% of the radioactivity injected as lysophosphatidylcholine had been taken up by the brain. 3. Approx. 70% of the doubly labelled lysophosphatidylcholine taken up by both grey and white matter was converted into phosphatidylcholine, whereas about 30% was hydrolysed. 4. The absence of significant radioactivity in the phosphatidylcholine, free fatty acid and water-soluble fractions of plasma up to 30min after injection of doubly labelled lysophosphatidylcholine rules out the possibility that the rapid labelling of these compounds in brain could be due to uptake from or exchange with their counterparts in plasma. 5. The similarity between the (3)H:(14)C ratios of brain phosphatidylcholine and injected lysophosphatidylcholine demonstrates that formation of the former occurred predominantly via direct acylation. 6. Analysis of the water-soluble products from lysophosphatidylcholine catabolism revealed that appreciable glycerophosphoryl-[Me-(3)H]choline did not accumulate in the brain and that radioactivity was incorporated into choline, acetylcholine, phosphorylcholine and betaine. 7. The role of plasma lysophosphatidylcholine as both a precursor of brain phosphatidylcholine and a source of free choline for the brain is discussed.


Possible role of a thiol group in lactose synthetase A protein.


Hepatic and extrahepatic reduction of nicotine 1'-N-oxide in rats.


Effect of inducers on the metabolism and excretion of disodium cromoglycate in rats.


Potentiation and modulation of the immune response of guinea pigs to poorly immunogenic protein-hapten conjugates by pretreatment with the MER fraction of attenuated tubercle bacilli.


Asparagine transport in Lactobacillus plantarum and Streptococcus faecalis.


Estimates and estimation errors of hormone secretion, transport and disposal rates in the maternal-fetal system.


Development of cortisol binding in the rhesus monkey.


An analysis of the negative feedback control of gonadotropin secretion utilizing chronic implantation of ovarian steroids in ovariectomized rhesus monkeys.


Effect of synthetic TRF on prolactin release in the sheep.


Risks, research, and reason.

Risks, research, and reason.


Pancreatic biogenic amines and insulin secretion in health and disease.


Erythrocebus patas--an anatomically suitable primate model for reproductive studies.


Altered immune function in hypercholesterolemic monkeys. Humoral and cellular immune responses to several antigens were compared in control and hypercholesterolemic groups of monkeys. Chronic hypercholesterolemia, with concomitant hyperphospholipidemia and hypotriglyceridemia, was produced experimentally by feeding monkeys a high-fat, high-cholesterol diet. When studied prior to infection, hypercholesterolemic monkeys exhibited impaired development of precipitating antibodies against ovalbumin, enhanced sensitivity to tuberculin antigen (stimulated apparently by mycobacterial components in complete Freund adjuvant), and an increased rate of clearance of colloidal carbon from blood. During pneumococcal infection the ability of neutrophiles from hypercholesterolemic monkeys to reduce nitroblue tetrazolium dye showed an increase greater than that of control monkeys; both groups exhibited increased but comparable final clearance rates of colloidal carbon, although the increment of increase was smaller in hypercholesterolemic monkeys.


Extracellular factor synthesized by Bacillus cereus which evokes a dermal reaction in guinea pigs. A guinea pig skin assay was developed as a measure of ileal loop fluid-inducing factor in the rabbit and possibly of the causative principle of Bacillus cereus food poisoning. A green or bloody necrotic reaction was produced in the guinea pig skin by injection of culture filtrates from 21 of 24 B. cereus strains tested, and by no other Bacillus species tested except for the closely related B. thuringiensis. The skin factor was synthesized and excreted by logarithmically growing cells, inactivated by heating at 56 C for 5 min, precipitable by ammonium sulfate, and was nondialyzable. The skin activity was not related to the lecithinolytic or hemolytic activities of B. cereus. Production of the skin factor depended on the medium in which the culture was growing. The factor was an antigenically active substance. There are indications that at least two antigenically distinct species of skin factors exist.


Effect of colon flora and short-chain fatty acids on growth in vitro of Pseudomonas aeruginsoa and Enterobacteriaceae. Heat-stable antibacterial activity in the following suspensions was demonstrated against Pseudomonas aeruginosa at pH 6.5, 6.0, and 5.5: (i) pooled colon contents of normal mice; (ii) an anaerobic, 48-h culture of normal mouse feces; and (iii) anaerobic, 48-h cultures of different bacteria from human colon flora (Escherichia coli, Bacteroides fragilis, Klebsiella pneumoniae, and Proteus mirabilis). The lower the pH of the medium, the greater was the antibacterial activity of these suspensions. The antibacterial activity of five fatty acids (propionic, butyric, isobutyric, acetic, and formic acids) was greater against P. aeruginosa than against three Enterobacteriaceae (E. coli, K. pneumoniae, and P. mirabilis) at all fatty acid concentrations (0.16 M to 0.005 M) and at the 3 pH values studied (5.5, 6.0, and 6.5). As the pH value increased, the antibacterial activity decreased. Antibacterial activity was greater at higher fatty acid concentrations, and at each pH value it was greatest for the fatty acids having high pK(a) values. Lactic acid, with the lowest pK(a), exhibited little or no antibacterial activity. Acetic and butyric acids, two of the three predominant volatile fatty acids determined by gas chromatography in the mouse colon contents and in the anaerobic culture of mouse feces, occurred in vivo in concentrations which inhibited growth of P. aeruginosa in vitro at the pH of the mouse cecum. These results suggest that undissociated short-chain fatty acids produced by the colon flora may be a mechanism of intestinal resistance to colonization by P. aeruginosa.


Relationship between tuberculin hypersensitivity and cellular immunity to infection in mice vaccinated with viable attenuated Mycobacterial cells or with Mycobacterial ribonucleic acid preparations. The migration inhibition technique has been used to study delayed hypersensitivity in vitro by using peritoneal exudate cells and splenic lymphocytes from mice vaccinated with viable cells of the attenuated H37Ra strain of Mycobacterium tuberculosis and from mice vaccinated with ribonucleic acid (myc RNA) preparations obtained from viable mycobacterial cells of the same strain. Inhibition of macrophage migration was noted when purified protein derivative (PPD) or viable H37Ra cells were added to peritoneal exudate cells obtained from mice immunized with viable H37Ra cells and not from mice immunized with myc RNA. Splenic lymphocyte cultures were exposed to the same antigens in vitro. Filtered supernatant fluids from these lymphocyte cultures, when added to peritoneal exudate cells obtained from nonimmunized mice, inhibited migration only when they were obtained from lymphocytes which came from mice immunized with viable H37Ra cells. Injection of PPD intravenously into vaccinated mice resulted in inhibitory supernatant fluids from splenic lymphocyte cultures only when the lymphocytes came from mice immunized with viable H37Ra cells. However, intravenous injection of either viable H37Ra cells or of myc RNA preparations into mice vaccinated with myc RNA occasionally produced inhibitory supernatant fluids when lymphocytes were obtained from these mice. On the other hand, mice vaccinated with myc RNA or viable H37Ra cell preparations were consistently and equally protected against intravenous challenge with the virulent H37Rv strain. Thus, although some evidence was obtained for a delayed type hypersensitivity in mice vaccinated with H37Ra cells or with myc RNA to ribosomal proteins or other proteins associated with the RNA preparation, no evidence of tuberculin hypersensitivity could be detected in any mice vaccinated with the myc RNA. These results argue against a role for tuberculin hypersensitivity in immunity to tuberculous infection.


Metabolism of Richettsia tsutsugamushi and Rickettsia rickettsi in irradiated host cells. The metabolism of Rickettsia tsutsugamushi (Gilliam strain) multiplying in irradiated L cells was investigated by methods involving the use of (14)C-labeled substrates and cycloheximide, an inhibitor of eukaryotic metabolism. Cycloheximide-resistant amino acid and adenine incorporations were appreciably higher in infected than in uninfected cultures during the period from 3 to 5 or 6 days postinoculation. The metabolism of R. rickettsi was similarly studied in primary duck embryo cells, which are more susceptible to infection with this rickettsia than are L cells. A difference in cycloheximide-resistant activity between infected and uninfected cultures was also noted, but was small. This finding is attributed to the more limited growth of R. rickettsi.


Interaction of gonococci with phagocytic leukocytes from men and mice. The interaction of human and mouse phagocytic leukocytes with representative virulent (F62-T1) and avirulent (F62-T4, RD-5) strains of Neisseria gonorrhoeae was studied in vitro. Leukocyte monolayers were incubated with gonococci for 30 min at 37 C, washed repeatedly, reincubated with fresh medium, and sampled for viable bacteria at intervals. After the initial incubation period and washing, human leukocytes retained larger numbers of viable T1 than of T4. During the subsequent 120 min of incubation, the numbers of viable T1 remained approximately constant, whereas viable counts of T4 declined by about two-thirds. In contrast, mouse leukocytes under similar conditions destroyed 70% of both types of gonococci. When human bactericidal serum was applied to infected human leukocytes, it had no effect on T4 but inactivated over 50% of T1. It is concluded that T4 are phagocytized by human leukocytes and are thus exposed to internal digestion, but are protected from bactericidal serum. T1, on the other hand, either adhere to the surface of the leukocytes or remain located so that they are neither digested by the leukocytes nor protected from bactericidal serum.


Metabolism and excretion of exogenous thyrotropin-releasing hormone in humans. To study the metabolism of thyrotropin-releasing hormone (TRH) in vivo, 400 mug TRH was administered intravenously to eight normal male subjects. Multiple plasma and urine samples were obtained before and after TRH administration. Serum TSH concentrations increased after TRH administration in all subjects. Plasma TRH levels, measured by radioimmunoassay, were undetectable (< 0.4 ng/ml) before TRH administration. Plasma TRH concentrations averaged 33+/-7 ng/ml (mean +/-SEM) 2 min after TRH injection. Thereafter, they decreased rapidly so that the mean plasma TRH level was 2.9 ng/ml 20 min after TRH administration. The fall in plasma TRH levels was linear during this interval. Thereafter TRH levels declined more slowly. The mean half-life (t(1/2)) of TRH was 5.3+/-0.5 min. The mean distribution volume was 15.7+/-3.8 liters, an average of 16.5% of body weight in these subjects. In the urine, 5.5+/-0.9% of the administered TRH was recovered in the 3 h after TRH administration. Of the total urinary TRH recovered, 84.9% was excreted in the first 30 min. These results indicate that TRH is distributed in a large volume, that it is rapidly metabolized and that a significant quantity of administered TRH is excreted in the urine.


Endocrine studies in anencephaly.

Endocrine studies in anencephaly. Endocrine function has been investigated in four anencephalic neonates to determine the influence of absence of cortical and hypothalamic tissue and of hypoplasia of the pituitary. Intravenous glucose administration resulted in higher peak values for blood sugar and more rapid glucose disposal rates than reported in normals. Intravenous insulin tolerance tests on two of the infants failed to evoke elevations in plasma growth hormone, and the infants showed a remarkable resistance to the hypoglycemic effect of insulin. Administration of lysine-vasopressin caused an active growth hormone release. Similarly, there was a large increase in serum thyrotropin after administration of synthetic thyrotropin-releasing hormone. Basal levels of both thyrotropin and growth hormone were low as compared with values reported for normal newborns. Prolactin values obtained on three of the infants were in the normal range. The results strongly suggest that anterior pituitary function mediated by the hypothalamus and its releasing factors is deficient in anencephaly. However, the anterior pituitary can release growth hormone and thyrotropin when stimulated directly and, in the case of thyrotropin release, may function autonomously. The normal prolactin values presumably reflect the absence of the hypothalamic prolactin inhibitory factor.


The association of respiratory infection, recurrent hematuria, and focal glomerulonephritis with activation of the complement system in the cold. The study of the activation of C3, C5, and C7 associated with the conversion of C3 in the serum of a 9-yr old girl after incubation at 0 degrees C for 6-8 h without utilization of C1, C4, and C2 is described. The patient has upper respiratory infections associated with recurrent gross hematuria, focal glomerulonephritis, and transient renal insufficiency. Histological lesions demonstrated the presence of B1c globulin IgA and properdin in the glomeruli. The activation of complement (C) in the cold requires the patient's IgA. Removal of IgA from the serum by immunoadsorption prevents activation and conversion of C3. Bactericidal and phagocytic activity is also impaired after incubation. C3 proactivator (C3PA) level is reduced before and after incubation. Properdin level drops after incubation. These findings suggest that the activation of C3 which is demonstrable in vitro may be a continuous process in vivo.


Isolation of Neisseria lactamicus from the nasopharynx. During 1971 and 1972, 71 cultures of neisseriae that attacked lactose were received by this laboratory. All strains except one from an eye swab were from the nasopharynx of healthy subjects. Nineteen similar strains from the nasopharynx were isolated in this laboratory. The characteristics of these strains were compared with those of Neisseria meningitidis, Neisseria pharyngis, Neisseria catarrhalis, and Neisseria lactamicus. The 90 strains under investigation closely resembled Neisseria meningitidis but could be differentiated by production of acid from lactose and beta-galactosidase activity and were therefore classified as Neisseria lactamicus.


Cross immunity studies with East and West African strains of Plasmodium falciparum in owl monkeys (Aotus trivirgatus).


Evaluation of the complement fixation test in diagnosis of actinomycetoma.


Autoantibodies in schistosomal liver fibrosis.


Neonatal gonococcal infection. I. Orogastric contamination with Neisseria gonorrhoea.


Free fatty acid content of cacao beans infested with storage fungi.


Effect of MDMP on protein synthesis in wheat and bacteria.


Altered enzyme molecules in senescent organisms: mouse muscle aldolase.


An angiographic study of the meningorachidian venous system.


Simple measurement of chest-wall thickness with ultrasound.


Reemphasis on tuberculosis.

Reemphasis on tuberculosis.


Accommodative vergence in the alert monkey. Motor unit analysis.


Early malnutrition and brain development.


The neurocranial basis for facial form and pattern.


Environmental factors internal to the host that affect the sucess of microbial insecticides.


Effects of entomopathogens on vertebrates.


Enzootic diseases of insects.

Enzootic diseases of insects.


Environmental impact of insect control by microorganisms.


Perspectives of biological insect control.


Means for insect regulation. Microbial toxins.


Determination of the molecular weight in nucleic acids by end-phosphate analysis.


Properties of oligodeoxynucleotides that determine priming activity with Escherichia coli deoxyribonucleic acid polymerase I.


The role of nutrition in the physiological and behavioral effects of early maternal separation on infant rats.


Electron microscopy of Bacillus subtilis protoplast membrane after treatment with phospholipase A 2  and phospholipase C.


Electrocardiographic and cardiac morphological changes associated with environmental stress in squirrel monkeys.


The effects of propionate on sodium transport by the toad bladder. Evidence for a metabolic mode of action.


Permeability of Azotobacter vinelandii to cations and anions.


Adenine nucleotide metabolism of blood platelets. 8. Transport of adenine into human platelets.


Denaturation and reactivation of human carbonic anhydrases in guanidine hydrochloride and urea.


Detection of proteolytic activity after isoelectric focusing in polyacrylamide gel.


Modified erythrocytes in indirect haemagglutination with Neisseria gonorrhoeae antigen.


Penicillin sensitivity of gonococci in Ethiopia.


On the localization of ribonucleases in bacteria.


Intrarenal distribution of blood flow in the baboon: effect of noradrenaline and adrenergic blocking agents.


Skeletal muscle phosphorylase kinase deficiency: detection of a protein lacking any activity in ICR-IAn mice.


Brief communication: processed soya flour as substitute for dry skim milk in the "protein packet" in the treatment of protein calorie malnutrition.


Nutritional status of rural pre-school children in West Bengal: II clinical observations.


Effect of Aspergillus flavus mycotoxin on Culex mosquito larvae.


Larval mortality of Culex tarsalis and Aedes aegypti when reared with different concentrations of Tetrahymena pyriformis.


Identification of members of the Culex pipiens complex by immunodiffusion and immunoelectrophoresis.


Clinical response to disodium cromoglycate. A double blind study.


Factors affecting the turnover of cerebral glycogen and limit dextrin in vivo.


Isolation and characterization of an organ specific ribonucleoprotein from goat brain.


Lysolecithin and sphingosinephosphoryl-choline in the metabolism of brain phospholipids of the rhesus monkey (Macaca mulatta): effects of development.


Lactose content of tube feeding diets as a cause of diarrhea.


The treatment of self-induced photic epilepsy.


Reconsideration of the role of serotonin in subarachnoid haemorrhage.


Immunologic relationships of antihemophilic factor of different species detected by specific human and rabbit antibodies.


The use of an immunoadsorbent in the isolation of Australia antigen.


Tissue and organ culture studies of hepatitis type B.


Radioimmunoprecipitation assay for Australia antibody.


Development of antilymphocyte sera by cross immunization of chimpanzees and baboons.


Alveolar bone changes consequent to orthodontic forces. A tetracycline marking study.


Hepatitis: intrahepatic expression of Au antigen and immune complexes in liver tissue.


Australia antigen in cord blood.

Australia antigen in cord blood.


Blood bank service screening of Australia antigen and antibodies at Mainz, Germany.


Australia antigen and antibody in laboratory and other hospital personnel.


Repeated monitoring of Au-SH antigen in blood donors of Reggio Emilia.


Low voltage counterimmunoelectrophoresis for the detection of Australia antigen.


Amodification of the immunoelectrophoretic method in the detection of Australia antigen.


Primates as model for human viral hepatitis: transmission of infection by human hepatitis B virus.


Gas-chromatographic determination of selenium.


Arthritis and HB Ag-positive hepatitis.

Arthritis and HB Ag-positive hepatitis.


Antithymocyte antisera globulin effect on human lymphoid neoplasia.


Steroid-responsive hypercalcemia in disseminated bone tuberculosis.


Metabolic and microcirculatory effects of glucagon in hypovolemic shock.


Experience with elemental diet in the treatment of inflammatory bowel disease. Is this primary therapy?


Energy-dependent accumulation of iron by isolated rat liver mitochondria. I. General features.


Light-induced changes in the fluorescence yield of chlorophyll a in Anacystis nidulans. I. Relationship of slow fluorescence changes with structural changes.


Chromatographic separation of two ionic strength dependent conformations of valine transfer ribonucleic acid of Bacillus subtilis.


Selective reaction of glyoxal with guanine residues in native and denatured Escherichia coli 5S RNA.


Formation of ribosomal particles in virginiamycin sensitive and resistant mutants of Bacillus subtilis.


A comparative clinical evaluation of hepatitis-associated antigen detection.


Salivary immunoglobulins in Sjögren's syndrome.


Measurements of concentrations of human serum innunoglobulins.


Response of plasma histaminase activity to small doses of heparin in normal subjects and patients with hyperlipoproteinemia. The release of histaminase activity in plasma after small intravenous of heparin was studied in 85 normal subjects and patients. In normal subjects, plasma histaminase activity (basal level, 1.7+/-0.1 U/ml, mean +/-SEM) increased 1.6+/-0.2 U/ml after 10 U of heparin/kg, 8.5+/-2.4 U/ml after 20 U/kg, and 33+/-4.9 U/ml after 75 U/kg. The extent of the increase varied widely among individuals but in a particular individual the response was constant and dose-dependent. Histaminase activity rose to peak levels within 7-15 min and then declined exponentially with a half-life of 40-120 min. This pattern of response was also observed in two patients with the histaminase-producing tumor, medullary carcinoma of the thyroid. A significantly reduced response was observed, however, in 14 patients with type I hyperlipoproteinemia, a disorder in which high plasma triglyceride levels are associated with low postheparin plasma lipolytic activity. After 10 U heparin/kg, plasma histamine activity increased 0.5+/-0.2 U/ml, and after 75 U heparin/kg, 10.9+/-5.6 U/ml. In contrast, in 27 patients with other types of hyperlipoproteinemia in whom postheparin lipolytic activity was normal, the increase (2.4+/-0.6 U/ml) in plasma histaminase activity after 10 U heparin/kg was not significantly different from that of normal subjects. The reduced response of the plasma histaminase activity to heparin in patients with type I hyperlipoproteinemia did not appear to be due to the presence of lipemia or to an inhibitor of the enzyme in plasma. These findings suggest that many patients with type I hyperlipoproteinemia may have deficient release of both lipolytic and histaminase activities into plasma after heparin administration.


A saturable, vasopressin-sensitive carrier for urea and acetamide in the toad bladder epithelial cell. The permeability of the toad bladder to a series of isotopically labeled nonelectrolytes was determined in the presence of 150 mM unlabeled acetamide. Under these conditions, overall bladder function was unimpaired, as shown by a normal response to vasopressin of short-circuit current and permeability coefficient of [(3)H]water,[(14)C]ethanol, and [(14)C]propionamide. The permeability of the bladder to isotopic acetamide and urea, however, was significantly depressed by unlabeled acetamide, in both the absence and presence of vasopressin. These experiments indicate a competition between unlabeled and isotopic species for binding sites, and show the existence of a saturable, vasopressin-sensitive carrier for urea and acetamide in the epithelial cell membrane.


Cellular mediators of anti-Listeria immunity as an enlarged population of short lived, replicating T cells. Kinetics of their production. An intravenous immunizing infection with the facultative, intracellular parasite, Listeria monocytogenes results in the production in the spleen of a population of immunologically-committed lymphocytes which can adoptively immunize normal recipients against a lethal challenge infection. These cellular mediators of immunity are first produced in the spleen between days 2 and 4 of infection and reach peak production on day 6. Their production then progressively decreases until about day 20 when their presence can no longer be detected. Increased production of cellular mediators is coincident with major increases in cell division, cellularity, and spleen weight. Decreased production of cellular mediators, on the other hand, is associated with decreases in cell division, cellularity, and spleen weight. Again, the level of delayed sensitivity to Listeria antigens expressed by the host at any one time is proportional to the number of cellular mediators in the spleen. Increased production of cellular mediators is also associated with major increases in the total numbers of replicating T cells and B cells in the spleen. That the cellular mediators of immunity are part of the replicating T cell population, rather than the B cell population, is evidenced by their susceptibility to anti-theta serum and by their resistance to anti-Ig serum. Furthermore, they can be completely eliminated from the spleen by a brief pulse of the antimitotic drug, vinblastine. This study allows the conclusion that the cellular mediators of anti-Listeria immunity belong to an expanded population of rapidly dividing, short-lived T cells. It is suggested that they have the same properties as the T cell effectors of allograft immunity.


Immunologic studies of the major nonimmunoglobulin protein of amyloid. I. Identification and partial characterization of a related serum component. Antisera have been prepared against the major nonimmunoglobulin component of secondary and familial Mediterranean fever (FMF) associated amyloid which has been called A component or acid soluble fraction (ASF). The antisera were shown to be monospecific for ASF by precipitation of (125)I-labeled antigen and gave a reaction of identity with four different ASF preparations. The antisera were able to detect a circulating component in human serum that migrated in the alpha1-globulin region. This circulating component gave a line of identity with degraded ASF by double immunodiffusion. 57 normal sera and 89 sera from patients with diseases known to be frequently associated with amyloidosis were tested by immunodiffusion for the circulating ASF component. 7% of normal sera and 50-80% of the pathologic sera had elevated amounts of this component. Absorption studies showed that all normal sera probably have small amounts of this component while cord sera do not have detectable amounts. This component was partially purified and was shown to be slightly larger than albumin. The relation of the circulating component to the acid soluble fraction of amyloid is discussed.


Phylogenetically associated residues within the VH3 subgroup of several mammalian species. Evidence for a "pauci-gene" basis for antibody diversity. Immunoglobulin heavy chains from IgG pools of several mammalian species have been subjected to Edman degradation on an automated protein sequencer. The percentage of unblocked vs. blocked heavy chains was estimated from the yield of the invariant valine in the second position. Further analysis of these unblocked polypeptides unequivocally placed them in the V(HIII) subgroup on the basis of homology with known human heavy chain sequences. The mammals studied could be divided into three distinct categories on the basis of the distribution of the V(HIII) subgroup. In several species the V(HIII) subgroup could not be detected while, in others, virtually all of the heavy chains belonged to this subgroup. Several species had intermediate amounts with the level of the V(HIII) subgroup restricted to between 19 and 29% of the total pool. Within experimental error, all members of a given order had a similar V(HIII) subgroup distribution. Further amino acid sequence studies illustrated a high degree of structural homogeneity in the heavy chains of IgG isolated from pooled sera of a number of mammalian species. The very close amino acid sequence homologies of the amino terminal 24 residues of the various pools corroborated conclusions previously obtained using several myeloma proteins from some of these same species. In particular, certain phylogenetically associated residues were identifiable at characteristic positions in the pools in confirmation of their identification in the myeloma proteins. The simplest assumptions would suggest that these findings are more compatible with a pauci-gene than a multi-gene basis for the generation of antibody diversity.


The nature of the antibody response to Yersinia enterocolitica serotype IX in cattle. The nature of the antibody response of cattle to the antigen of Yersinia enterocolitica IX cross-reacting with Brucella spp. was examined. Density-gradient ultracentrifugation, ion-exchange chromatography, antibody adsorption and elution and disulphide bond reduction tests showed that both 19 S IgM and 7 S IgG(1) and IgG(2) antibodies were produced in response to the cross-reacting antigen. The highest titres of cross-reacting antibodies were detected by the agglutination and Coombs antiglobulin tests. Production of complement-fixing and precipitating antibodies cross-reacting with Br. abortus was transient and high titres were not attained.In contrast, although infection with Br. abortus also evoked cross-reacting antibodies of the IgM and IgG classes, much higher titres were produced in the complement fixation and precipitation tests and these persisted for long periods. At all stages of the serological response to both organisms, the two infections could be differentiated by the quantitative Rose Bengal plate test.


Typing of Pseudomonas aeruginosa: comparison of the phage procedure with the pyocine technique. Two hundred and sixty strains of Pseudomonas aeruginosa were isolated from patients of the Asaf Harofe Government Hospital. The strains were typed by phage technique and 64 of them were also typed according to pyocine sensitivity. The two methods proved complementary, and reduced the number of untypable strains. Phage typing was performed with both routine test dilution (RTD) and more concentrated phage suspensions. The most reliable results were obtained at 100 RTD.


Oral microbial profile of the marmoset.

Oral microbial profile of the marmoset.


A common antigenic constituent in various purified Salmonella endotoxins.


Host resistance to bacterial endotoxemia: mechanisms in endotoxin-tolerant animals.


Factors in normal human serum that promote bacterial phagocytosis.


Escherichia coli bacteremia in the squirrel monkey: demonstration of a complement-dependent neutrophil response.


Head injury in the chimpanzee. 1. Biodynamics of traumatic unconsciousness.


Head injury in the chimpanzee. 2. Spontaneous and evoked epidural potentials as indices of injury severity.


Feedlot performance and blood plasma amino acid patterns in beef steers fed diethylstilbestrol under ad libitum, restricted, and compensatory conditions.


Effects of dietary zinc on zinc enzymes in the rat.


Long-standing traumatic lesions of the hypothalamus.


Solubility of calcified dental tissues and of calculus in EDTA and 2 percent NaF.


Effect of chelating agents on chromium absorption in rats.


Influence of dietary protein deficiency on phagocytic activity of the reticulo-endothelial cells.


Neurofibromatosis of the bladder.

Neurofibromatosis of the bladder.


Self-administration of acetylsalicylic acid and combinations with codeine and caffeine in rhesus monkeys.


Presence of beta-aspartyl N-acetyl glucosamine amido hydrolase in mammalian spermatozoa.


Release of LH by synthetic LH-RH in the monkey, Macaca fascicularis.


Cellular location of the lipoteichoic acids of Lactobacillus fermenti NCTC 6991 and Lactobacillus casei NCTC 6375.


The association of zinc with some components of boar vesicular secretion and seminal plasma. I. Gel-filtration and dialysis studies.


Brain-damaging potential of protein hydrolysates.


Public accountability and reporting systems in Medicare and other health programs.


Safety of hydrolysates in parenteral nutrition.


Positive brain scans in children with idiopathic focal epileptic seizures.


Transmissible mink encephalopathy agent in squirrel monkeys. Serial electroencephalographic, clinical and pathologic studies.


Control circuits for determination and transdetermination.


Cytological, biochemical and bacteriological data of the hemolymph of Vespaorientalis.


Factors influencing neonatal infection by hepatitis B virus.


The need for further data.

The need for further data.


Fabry's disease: general clinical aspects.


A cytogenetic analysis of oocytes from Macaca mulatta and nemestrina matured in vitro.


Detection of Australia antigen in Indian childhood cirrhosis.


Role of bound ADP in photosynthetic ATP formation by chromatophores from Rhodospirillum rubrum.


Internal localization of nucleoside-catabolic enzymes in Escherichia coli.


Two types of soluble malic enzyme in rat tissues.


Supervoltage orbital radiotherapy for Graves' ophthalmopathy.


Serum levels of luteinizing hormone, follicle-stimulating hormone and progesterone throughout the mentrual cycle of rhesus monkeys.


Progressive decrease in serum triiodothyronine concentrations with human aging: radioimmunoassay following extraction of serum.


Immunoassay of thyroxine in unextracted human serum.


Elevations in plasma growth hormone concentration after luteinizing hormone-releasing hormone (LRH) in patients with active acromegaly.


Light induced changes in ionic flux in the retinal rod.


Synthesis of lysine from alpha, epsilon-diaminopimelic acid by mixed ciliated rumen protozoa.


A goitrous subject with defective synthesis of diiodotyrosine due to thyroglobulin abnormalities.


Gonococcal infections in the female.

Gonococcal infections in the female.


Gonorrhea among females in a military population.


Photoreactivation of Drosophila melanogaster embryonic cells.


Gestational meconium in the high-risk pregnancy.


Gap junctions between photoreceptor cells in the vertebrate retina. In the outer plexiform layer of the retina the synaptic endings of cone cells make specialized junctions with each other and with the endings of rod cells. The ultrastructure of these interreceptor junctions is described in retinas of monkeys, rabbits, and turtles, in thin sections of embedded specimens and by the freeze-fracturing technique. Cone-to-rod junctions are ribbon-like areas of close membrane approximation. On either side of the narrowing of the intercellular space, the junctional membranes contain a row of particles located on the fracture face A (cytoplasmic leaflet), while the complementary element, a row of single depressions, is located on fracture face B. The particle rows are surrounded by a membrane region that is devoid of particulate inclusions and bears an adherent layer of dense cytoplasmic material. Cone-to-cone junctions in some places are identical to cone-to-rod junctions, while in other places they closely resemble typical gap junctions (nexus). Interreceptor junctions, therefore, represent a morphological variant of the gap junction, and probably mediate electrotonic coupling between neighboring photoreceptor cells.


The use of iodinated RNA for gene localization. Carrier-free iodination of 5S RNA with (125)I yields a probe suitable for use in cytological RNA-DNA hybridization studies. The genes coding for 5S ribosomal RNA in Drosophila could be localized by autoradiography after a 2-day exposure, whereas a 2-month exposure is needed when the best available [(3)H]RNA is used. The procedure introduces one covalently bound (125)I atom per 100 nucleotides, resulting in a specific activity of over 10(8) dpm/mug of RNA. The method may be readily applied to other problems involving molecular hybridization techniques.


Relative orientation with respect to the centromere of ribosomal RNA genes of the X and Y chromosomes of Drosophila melanogaster. We tested for recombination within the DNA that codes for ribosomal RNA (rDNA) on the X and Y chromosomes of Drosophila melanogaster. The criterion was the appearance of intermediate bb alleles on X-Y recombinants from wild bb(+) and lethal bb(1) loci carried on parental chromosomes. Recombination within the rDNA occurs only when the rDNA of the X chromosome is inverted. We conclude that rDNAs of normal X and Y chromosomes have opposite orientation with respect to the centromere. The implications of this observation are discussed.


Loss of the sigma activity of RNA polymerase of Bacillus subtilis during sporulation. The activity of the sigma subunit of the RNA polymerase of Bacillus subtilis decreases markedly during the first 2 hr of sporulation. Moreover, sigma activity remains deficient throughout the sporulation process and in dormant spores. The time course of changes in RNA polymerase during sporulation indicates that alterations in the core of RNA polymerase occur after the loss of sigma activity. Core RNA polymerase purified after the second and before the ninth hour of sporulation fails to respond to vegetative sigma subunit in vitro and contains variable amounts of a 110,000-dalton polypeptide in place of the beta' subunit. Core RNA polymerase purified from dormant spores has a subunit structure indistinguishable from vegetative core enzyme.RNA polymerase purified by antibody precipitation from an extract of a mixture of sporulating and excess vegetative cells separately labeled with two different radioisotopes contains beta' subunit and no 110,000-dalton polypeptide. However, RNA polymerase purified from sporulating bacteria in the absence of excess vegetative cells progressively loses the beta' subunit at each stage of purification even in the presence of the protease inhibitor, phenylmethyl sulfonyl fluoride. These findings suggest that the alteration of the beta' subunit is due to proteolysis in vitro.


The aflatoxins and human liver cancer.

The aflatoxins and human liver cancer.


Evaluation of environmental health programmes: report of a WHO scientific group.


Microangiopathy in the experimentally diabetic animal.


Microangiopathy in animals with spontaneous diabetes.


Pseudo-leukocytosis associated with cryoglobulinemia.


Immune serum globulin--a cause for anti-Rh (D) passive sensitization.


Membrano-proliferative glomerulonephritis associated with persistent viral hepatitis.


"IGF" in food allergy?

"IGF" in food allergy?


Mechanisms of drug-enzyme interactions.

Mechanisms of drug-enzyme interactions.


Microcirculation of monkey pancreas with special reference to the insulo-acinar portal system. A scanning electron microscope study of vascular casts.


Effects of perhexiline maleate in patients with angina pectoris unrelieved by other antianginal agent.


The effect of trimazosin in essential hypertension.


Double-blind clinical evaluation of mazindol, dextroamphetamine, and placebo in treatment of exogenous obesity.


Response to a single intravenous dose versus multiple intramuscular administration of iron-dextran complex: a comparative study.


Oral aminophylline and dikydroxypropyl theophylline in reversible obstructive airway disease: a single-dose, double-blind, crossover comparison.


A double-blind comparison of ethoheptazine citrate, propoxyphene hydrochloride, and placebo.


Clinical trial of a new anti-inflammatory drug in otolaryngology.


Irradiation synovectomy: clinical study of 67 knee effusions intra-articularly irradiated with  90 Y resin.


Influence of treatment on symptomatology and correlated electroencephalographic (EEG) changes in the aged.


Treatment of premenopausal endometrial hyperplasia by a combination of a long-acting estrogen and a cyclic progestogen.


The extrapyramidal neuroleptic syndrome: description and measurement.


Preanesthetic medication in elective surgery: comparison of intravenous diazepam with meperidine.


The use of N-demethyldiazepam in outpatients suffering from insomnia.


Clorazepate dipotassium, diazepam and placebo in chronic anxiety.


Metiapine in acute schizophrenic patients.


Metiapine: the evaluation of the antipsychotic properties of a new neuroleptic. A pilot study.


Comparable therapeutic efficacy of tryptophan and imipramine: average therapeutic ratings versus "true" equivalence. An important difference.


Potentiation of haloperidol by  -methyldopa in the treatment of schizophrenic patients.


The computer analyzes two psychotherapeutic agents in anxiety-depressed states.


Effects of molindone on CNS structures: neurophysiological and behavioral correlations.


A multicenter investigation of lorazepam in anxiety neurosis.


The protein needs of malnourished children.


Dipropylacetate (Dépakine, Ergenyl) in the treatment of epilepsy.


Superiority of clonazepam over diazepam in experimental epilepsy.


Principles of methodical studies on capillaries in the light of the immunomorphogenesis of the capillary-haemopoietic system. I. Immunobiogenetic studies.


Pattern formation in the blue-green alga, Anabaena. I. Basic mechanisms.


Intestinal carriage of Klebsiella and pseudomonas in burned children and their comparative role in nosocomial infection.


Temperature-sensitive RNA polymerase of a mammalian cell mutant.


Acetylcholinesterase in human erythroid cells.


Immunogenetic and electrophoretic studies with extracts of different adult Culex pipiens strains.


A comparative study of diamine oxidase in amniotic fluid and serum in normal pregnancy and in pregnancy complicated by rhesus iso-immunization.


Premature labour at Groote Schuur Hospital.


Dairy herd environment and the control of mastitis.


Plasma and urine ribonuclease as a measure of nutritional status in children.


Effects of dietary supplementation of monosodium glutamate on infant monkeys, weanling rats, and suckling mice.


Influence of a viral enteric infection on the aminogram and pathology of protein-calorie malnutrition in the pig.


Use of the indirect fluorescent antibody technique in serologic studies of Theileria lawrencei infections in cattle.


International and local growth standards.


Transmissible drug resistance in Enterobacteriaceae isolated from healthy nonhuman primates.


Naturally occurring cholelithiasis in a rhesus monkey and its effects on plasma and biliary lipid concentrations.


Characterization of Listeria monocytogenes serotype 5.


Familial chronic interstitial pneumonia.


A new "double ring" screening test for alpha1-antitrypsin variants.


Productivity of prolonged follow-up after chemotherapy for tuberculosis.


Primary drug-resistant tuberculosis in children, 1961 to 1971. A comparison of the frequency of primary drug-resistant tuberculosis among children observed during the period of transition from long-term to short-term hospitalization of adults with infectious tuberculosis.


A study of mycobacterial species isolated from patients with chronic respiratory disease.


Scanning electron microscopy of mycobacteria.


Effect of age on the fatty acids C14 to C19 of mycobacteria.


Species differences in the diurnal rhythmicity of courtship behaviour within the melanogaster group of the genus Drosophila.


Surgical anatomy of the biliary tract. As demonstrated by peroperative cholangiography.


The location of N-acetylgalactosamine in the walls of Bacillus subtilis 168. The N-acetylgalactosamine in the walls of Bacillus subtilis 168 occurs in two polymers. One of these contains N-acetylgalactosamine, glucose and phosphorus and is attached to the peptidoglycan through an alkali-labile bond; preliminary studies indicate that a repeating unit of this polymer is glucosyl-N-acetylgalactosamine 1-phosphate. N-Acetylgalactosamine is also associated with the peptidoglycan in a component that is not converted into the free sugar or other soluble compounds on treatment of the walls with alkali. The two polymers containing N-acetylgalactosamine are released on autolysis of the walls and can be separated by ion-exchange chromatography. As glucose 6-phosphate is produced by gentle hydrolysis of the wall with acid a third phosphate polymer, poly(glucose 1-phosphate), may occur in this wall. However, as no polymer with this structure could be separated from that containing galactosamine, its existence has not been established unequivocally. The methods described permit the study of variations in N-acetylgalactosamine content with respect to growth conditions.


Regulation of C metabolism by L-methionine in Saccharomyces cerevisiae. 1. The concentrations of folate derivatives in aerobic cultures of Saccharomyces cerevisiae (A.T.C.C. 9763) were determined by microbiological assay employing Lactobacillus casei (A.T.C.C. 7469) and Pediococcus cerevisiae (A.T.C.C. 8081). Cells cultured in media lacking l-methionine contained higher concentrations of folate derivatives than cells grown in the same media supplemented with 2.5mumol of l-methionine/ml. The concentrations of highly conjugated derivatives were also decreased by supplementing the growth medium with l-methionine. 2. DEAE-cellulose column chromatography of extracts prepared from cells grown under these conditions revealed that the concentrations of methylated tetrahydrofolates were drastically decreased by the methionine supplement. Smaller decreases were also observed in the concentrations of formylated and unsubstituted derivatives. 3. The concentrations of four enzymes of C(1) metabolism were compared after 6h of growth in the presence and in the absence of l-methionine (2.5mumol/ml). The specific activities of formyltetrahydrofolate synthetase, methylenetetrahydrofolate reductase and serine hydroxymethyltransferase were not altered by this treatment but that of 5-methyltetrahydrofolate-homocysteine methyltransferase was decreased by approx. 65% when l-methionine was supplied. The activities of 5-methyltetrahydrofolate-homocysteine methyltransferase, serine hydroxymethyltransferase and formyltetrahydrofolate synthetase were not appreciably altered by l-methionine in vitro. In contrast this amino acid was found to inhibit the activity of methylenetetrahydrofolate reductase. 4. Feeding experiments employing sodium [(14)C]formate indicated that cells grown in the presence of exogenous methionine, although having less ability to convert formate into methionine, readily incorporated (14)C into serine and the adenosyl moiety of S-adenosylmethionine. 5. It is suggested that exogenous l-methionine controls C(1) metabolism in Saccharomyces principally by regulation of methyl-group biogenesis within the folate pool.


A possible ribosomal-directed regulatory system in Euglena gracilis. Carbon dioxide fixation. It was shown that cycloheximide inhibits CO(2) fixation in Euglena cells in the dark, but no effect of chloramphenicol was found. The light-dependent CO(2) fixation was inhibited by chloramphenicol and by large amounts of cycloheximide, but was stimulated by small amounts of cycloheximide. The presence of the stimulatory concentration of cycloheximide abolished the inhibition effect of high concentrations of chloramphenicol. The results indicate that the light-dependent CO(2) fixation is controlled by a repression-derepression regulatory system, which seems to be independent of chlorophyll synthesis.


A possible ribosomal-directed regulatory system in Euglena gracilis. Chlorophyll synthesis. Cycloheximide at concentrations of 0.1-100mum stimulated chlorophyll synthesis when dark-grown cells of Euglena were illuminated. Chloramphenicol (1-4mm) inhibited chlorophyll synthesis. The effect of cycloheximide on the incorporation of [(14)C]leucine into material insoluble in trichloroacetic acid, and its failure to affect the incorporation of [(32)P]orthophosphate into such material in short incubations, are interpreted as evidence that cycloheximide specifically inhibits protein synthesis by 80S ribosomes. Since the inhibitory effect of chloramphenicol on chlorophyll synthesis is counteracted by the presence of cycloheximide, it is suggested that chlorophyll synthesis is subject to control by a cytoplasmic repressor synthesized on 80S ribosomes, and to a de-repressor synthesized on 70S ribosomes.


Kinetics of the diamine oxidase reaction. 1. The oxidation of p-dimethylaminomethylbenzylamine was followed spectrophotometrically by measuring the change in E(250) caused by the p-dimethylaminomethylbenzaldehyde produced under a wide variety of experimental conditions. 2. The effect of variations in concentrations of both substrates (amine and oxygen) and all products (aminoaldehyde, hydrogen peroxide and ammonia) on this reaction was studied and the results used to develop a formal mechanism. 3. The nature of the rate-limiting step was elucidated by studying the effects of alterations in ionic strength, dielectric constant and deuterium substitution on the velocity of the forward reaction. 4. Thermodynamic activation energy parameters were obtained at several pH values from the effects of temperature on the reaction.


Selective extraction of polymers from cell walls of gram-positive bacteria. Brief heating of Bacillus Licheniformis cell walls at 100 degrees C in aqueous buffers of pH3.0-4.0 removes some polymers but not others from the mucopeptides. For example, relatively undegraded teichuronic acid can be extracted at 100 degrees C in 20min at pH3.0 whereas the teichoic acids are not removed. Similar specificity can be shown with walls from three other species of micro-organism.


Comparative study of the optical rotatory dispersion and circular dichroism of human erythrocyte carbonic anhydrases A and B, and conformational variants B1 and B2.


Metabolic studies of (75Se)selenomethionine and (75Se)selenite in the rat.


Protein requirements of growing lambs.

Protein requirements of growing lambs.


A device and technique for urethrography in the female.


Osteoporosis and long-term corticosteroid therapy. Sequential measurements of total body calcium by in-vivo neutron activation analysis were made in five patients treated with corticosteroid drugs for severe inflammatory disease. No evidence of osteoporosis was seen to develop during the course of 20-33 months of study. In one case a substantial rise in total body calcium was found.


Significance of hyperamylasaemia and abdominal pain in diabetic ketoacidosis. An analysis of 35 consecutive episodes of diabetic ketoacidosis confirmed the frequent high levels of serum amylase in this condition. Serum amylase was raised during 21 episodes (60%), and in six instances (17%) the peak level exceeded 1,000 Somogyi units per 100 ml. Hyperamylasaemia was more often found when the initial blood sugar exceeded 500 mg/100 ml, or when the onset of the episode had been relatively acute (less than 48 hours). There was no conclusive evidence in any patient to support a diagnosis of acute pancreatitis and other explanations for the hyperamylasaemia are discussed. Even grossly raised amylase levels were not associated with increased mortality or morbidity.


Drugs for angina pectoris.

Drugs for angina pectoris.


Retrograde transneuronal degeneration of optic fibers and their terminals in lateral geniculate nucleus of rhesus monkey.


Optic chiasm section affects discriminability of asymmetric patterns by monkeys.


Haptenic activity of L-phenylalanine mustard.


Xenotransplantation of established human endometrial cells into newborn hamsters.


Clinical laboratory use of differential light scattering. I. Antibiotic susceptibility testing.


Uptake of protein in swallowed amniotic fluid by monkey fetal intestine in utero.


Bacteriological evaluations of swine exposed to lethal levels of gamma radiation.


Chelated trace minerals for G-F swine.

Chelated trace minerals for G-F swine.


Physico-chemical characteristics and regional distribution studies of GP-350, a soluble sialoglycoprotein from brain.


Immunochemical studies on glutamic acid decarboxylase (EC 4.1.1.15) from mouse brain.


The effects of changes in arterial plasma calcium concentration on submandibular secretory potentials. 1. The concentration of Ca in the arterial blood plasma perfusing submandibular glands of anaesthetized cats and possums (Trichosurus vulpecula) was altered by constant-rate close arterial injection of isotonic CaCl(2) or EDTA solutions.2. There were no significant changes in the resting potentials of surface acinar cells in either species when the arterial plasma Ca concentration was altered over the range 0-30 mM.3. Secretory potentials in response to parasympathetic stimuli and close arterial injection of ACh were reduced and finally abolished during EDTA injection, but temporarily restored by injection of CaCl(2).4. Secretory potentials in response to 1 Hz parasympathetic stimuli in the cat were enhanced by increasing the gland arterial Ca concentration up to 5 mM; but responses to 10 Hz stimuli or close arterial ACh injection were not affected. There was no such enhancement of secretory potentials in the possum.5. Increase in the gland arterial plasma Ca concentration above 5 mM caused reduction in the amplitudes of the secretory potentials to all stimuli in both species, until they were abolished at 7-10 mM in the possum and 15-20 mM in the cat.6. In both species, increase in the gland arterial plasma Ca concentration caused increase in the gland blood flow and enhancement of the vasodilator effects of parasympathetic stimuli and ACh injection. EDTA injection was sometimes associated with a vasoconstrictor response to parasympathetic stimuli.7. It is concluded that the secretory potentials, but not the resting potentials, of submandibular secretory cells require the presence of Ca in the interstitial fluid. It is suggested that Ca affects both the release of transmitter and at higher concentration the mechanism by which it activates the secretory cells.


Functional studies of splenic and internal mammary artery implants in canine hearts.