Immune response to Listeria monocytogenes in rabbits and humans. Rabbits were immunized with listeria antigens, staphylococcus antigen, or with both, and the course of their immune response was monitored. Antibodies to Listeria and Staphylococcus were produced in both immunoglobulin M (IgM) and immunoglobulin G (IgG) classes in response to inoculation with the specific antigen. Cross-responses occurred in rabbits injected only with Listeria or only with Staphylococcus, as well as in rabbits injected with both antigens. L. monocytogenes serotype 4d appeared to be immunologically distinct from L. monocytogenes serotype 2 and its cross-reaction with S. aureus. Human sera from bacteriologically confirmed cases of listeriosis were examined to determine the nature of the immunological response of man to Listeria. In the sera studied, IgM was the predominant antibody produced to Listeria, whereas cross-reactions with Staphylococcus were observed in both the IgM and the IgG antibody classes.


Resistance to gonorrhea possibly mediated by bacterial interference. Two men with no prior history of urethritis failed to develop gonorrhea after sexual exposures to women with genital gonococcal infection. Usual methods of prophylaxis, such as antibiotics or condoms, were not employed. The aerobic bacterial flora of these men's urethras consisted of several bacteria, some of which inhibited the in vitro growth of Neisseria gonorrhoeae. The hypothesis is suggested that bacterial interference may have played a role in protecting these men from gonorrhea.


Rapid detection of Clostridium botulinum toxin by capillary tube diffusion. A micro capillary agar-gel diffusion system for the detection of botulinal toxin in foods and cultures was developed and evaluated. Toxins types A, B, and E, produced in culture broth with and without added trypsin, and type E toxin, produced in inoculated canned clams, were tested with this system and with the mouse bioassay procedure. With nontrypsinized toxin, the capillary diffusion system detected as little as 100 minimal lethal doses (MLD) per ml but was effective only at higher levels, 10(6) to 1.5 x 10(7) MLD/ml, when used with trypsinized toxin. The inability to detect lower levels of trypsinized toxin was due to thioglycolate present in the medium used to produce toxin. Evidently, trypsinization of toxin produces polypeptides still held together by disulfide bonds. Cleavage of these bonds by reduction with thioglycolate reduces the sensitivity of the capillary method. Trypsinized toxin produced in broth without thioglycolate was detected as readily as nontrypsinized toxin. Toxin was detected in canned clams containing as low as 100 MLD/ml. No cross-reactions were observed with type E toxin and types A and B antitoxins. Extensive studies using the capillary method for detecting types A and B toxins were not performed; however, a suspected sample of commercially canned mushrooms gave a positive type B reaction but not a type A reaction. This typing was confirmed later by the mouse bioassay. Toxin was present at a level of 100 MLD/ml. The procedure developed may prove useful as a rapid screening method for the detection of botulinal toxin in foods, with final identification made by using the mouse bioassay.


NYC medium for simultaneous isolation of Neisseria gonorrhoeae, large-colony mycoplasmas, and T-mycoplasmas. NYC medium, primarily designed for isolation of pathogenic Neisseria, also readily supports the growth of large-colony mycoplasmas and T-mycoplasmas. When compared with A-3 agar medium and PPLO agar in clinical field trials, NYC medium performed equally as well as these mycoplasma-specific media in providing recovery of Mycoplasma species from female genital specimens. The transparent, highly-selective NYC medium permits direct, microscopic observation and presumptive identification of mycoplasmas, as well as Neisseria gonorrhoeae, without interference from contaminating saprophytes. As a single medium it can therefore be doubly useful in the diagnosis of gonorrhea and in the recognition of active or asymptomatic mycoplasma infections. Used in gonorrhea screening programs, the medium can be valuable in establishing the frequency of association of mycoplasmas with urogenital tract infection.


Retention of 14C-labeled tuberculin purified protein derivative in the skin of sensitized and nonsensitized animals. Tuberculin purified protein derivative labeled with (14)C ([(14)C]PPD) with a biological potency equivalent to the International Standard for tuberculin PPD was used to study the retention of tuberculin PPD in the skin of sensitized and nonsensitized animals. We found that [(14)C]PPD was almost entirely cleared from the skin test site during the first 18 to 24 h after injection and that when approximately 5% of the initial concentration of [(14)C]PPD was present in the skin test site, the size of the tuberculin skin reaction in sensitized guinea pigs was at its maximum. Furthermore, the addition of 5 or 50 mug of Tween 80 per ml to a solution of PPD did not change either the rate of clearance of PPD from the skin test sites of sensitized guinea pigs or the size of the tuberculin skin reactions. There was no difference in the rate of clearance of [(14)C]PPD from the skin test sites between sensitized and nonsensitized guinea pigs and between guinea pigs of different age. However, there was a significant difference in the rate of clearance of [(14)C]PPD between the guinea pig and the mouse. Finally, the percentage of [(14)C]PPD retained in the site of injection at 24 h was in the neighborhood of 5% of the initial concentration of the solution of PPD injected. The significance of these phenomena is discussed.


Aflatoxin-producing strains of Aspergillus flavus detected by fluorescence of agar medium under ultraviolet light. The fluorescence method of detecting aflatoxin-producing strains of Aspergillus flavus and related species utilizes the ultraviolet-induced fluorescence of aflatoxin produced in a modified Czapek's solution agar containing corn steep liquor, HgCl(2), and (NH(4))H(2)PO(4) instead of NaNO(3). The presence of aflatoxin is confirmed by thin-layer chromatography of CHCl(3) extracts of the fluorescing agar.


Plaque assay of rickettsiae in a mammalian cell line. Clear-cut and repeatable plaque assays were obtained for three rickettsiae of the spotted fever group (Rickettsia rickettsi, R. conori, and R. montana) in Vero cells used in a manner similar to that for arboviruses. In addition, three typhus group agents (R. typhi, R. canada, R. prowazeki) induced plaques in these cells. In preliminary tests Coxiella burneti (Nine Mile strain) failed to produce plaques. Comparable results were obtained in plastic flasks and plastic culture trays incubated in ambient air with or without addition of N-2-hydroxyethyl-piperazine-N'-2-ethanesulfinic acid buffer. Larger and more well defined R. rickettsi plaques were produced when cultures were overlaid with Leibovitz (L15) medium than with either medium 199 or Eagle medium. Phosphate-buffered saline containing bovine plasma albumin (fraction V), in contrast to brain heart infusion broth, as a diluent for preparing inocula consistently permitted development of larger and more numerous plaques with three agents: R. rickettsi, R. conori, and R. montana. When R. rickettsi and R. typhi were assayed in parallel in primary chicken embryo cultures and Vero cells, comparable results were obtained, but with R. canada results in Vero cells were superior. In contrast, R. prowazeki produced inconsistent results in Vero cells.


Epidemiological tracing of Pseudomonas aeruginosa: antibiogram and serotyping. The spread of a particular strain of Pseudomonas aeruginosa through a pediatric burn unit was monitored using serological typing and antibiotic susceptibility data.


Raman spectroscopy in vivo: evidence on the structure of dipicolinate in intact spores of Bacillus megaterium.


Anomalous molecular weights to proteases in gel chromatography.


Ribsome and messenger specificity in protein synthesis by bacteria.


Exploitation of the oxygen enhancement ratio in clinical practice.


Effects of radiation dose-rate from protracted, continuous irradiation to ultra-high dose-rates from pulsed accelerators.


Cortical motor representation of the laryngeal muscles in Macaca mulatta.


The postnatal development of antigens on mouse brain cell surfaces.


Metabolism of acid-soluble nucleotides in starved-refed-synchronized Tetrahymena pyriformis GL.


Genetic, cytological, and ultrastructural characterization of a temperature-sensitive lethal in Drosophila melanogaster.


Radioimmunoassay of rat parathyroid hormone in serum and tissue extracts.


The organization and gross mineralization patterns of the collagen fibres in Sharpey fibre bone.


Persistent abnormalities in pituitary function following neonatal thyrotoxicosis in the rat.


Stimulation of prolactin release in vivo by thyrotropin-releasing hormone (TRH) through direct action on the ectopic rat pituitary.


Neurological and psychomotor functions in children with an increased lead burden. Fifty-eight children with an increased lead burden underwent comprehensive investigations and were reevaluated 1(1/2) to 3 years later. Of these children 23-27% were noted to have minor neurological dysfunction and various forms of motor impairment during each evaluation. While the initial psychological assessment revealed low average mental abilities in the majority of children, during follow-up examination a significant increase in certain areas of intellectual functioning was observed.


Biochemical and toxicological response of infant baboons to lead driers in paint. In an effort to define the toxicology and disposition of lead compounds that presently exist in paint (i.e., organic driers), a controlled dose feeding study was initiated early this year with the use of 28 infant baboons as experimental animals. The infant baboon, established as a metabolic model for a child ingesting lead, will be used to determine the adequacy of present as well as recently recommended limitations for lead in paint to assure protection from this potential source of lead exposure. To accomplish this goal, research has been designed to determine basic dose-response relationships in animals ingesting constant daily doses of a dried paint, a lead octoate drier, and lead acetate. Doses for these compounds have been chosen to cover a broad range of concentrations including that recommended by the American Academy of Pediatrics from the maximum daily permissible lead ingestion, and associated estimates of paint intake by children with pica. PARAMETERS OF METABOLIC RESPONSE FOR EACH LEAD COMPOUND, INCLUDE: general clinical surveillance, lead concentrations in blood, urine and feces, erythrocytic delta-aminolevulinic acid dehydratase and free erythrocytic porphyrin. The response of several of these measures of lead exposure as a function of time will be discussed for each compound at the several dose levels administered.


Experimental acute lead encephalopathy in the juvenile rhesus monkey. Lead subacetate (0.5g) and 1000 units of vitamin D were given three times a week to four newly-weaned rhesus monkeys. In addition, two animals received only the vitamin D. The poisoned animals had an increase in the urinary excretion of delta-aminolevulinic acid, an elevated content of lead in the blood, and a fall in hemoglobin concentration. Between 6 and 18 weeks the animals suddenly developed ataxia, nystagmus, generalized weakness, and convulsions. At this time the animals were killed by perfusion of fixative and the brain prepared for light and electron microscopic studies. Definite morphological evidence of disease was confined to the central nervous system, except for one animal which showed the characteristic renal inclusions of lead poisoning. All animals showed PAS-positive globules associated with blood vessels and an exudative edema involving the white matter of the cerebral hemispheres and cerebellum. Ultra-structurally, this appeared as a granular precipitate within an expanded extracellular space. Alterations of nerve fibers were not seen in the white matter but axonal swelling was observed in the cerebral cortex. The perikaryon and neuropil appeared normal. The control animals showed no significant cerebral changes.


Influence of oxygen and pH on plating efficiency and colony development of WI-38 and Vero cells.


Pathobiological and behavioral effects of lead intoxication in the infant rhesus monkey. When infant rhesus monkeys were exposed to lead via the addition of lead acetate (0.5-9 mg/kg body weight) to their formula or by the consumption of lead particles from lead-based surrogate mothers, they developed symptoms of lead intoxication within 6 weeks. Seizures, muscular tremors, and altered social interaction were the predominant changes. Visual impairment was also apparent in the more severely affected animals. In the animals showing obvious symptoms lead levels varied between 300 to 500 mug/100 ml of blood. Even in those animals having blood lead levels below 100 mug, hyperactivity and insomnia were observed. When the exposure to lead was eliminated, seizures subsided and visual impairment was reduced; however, the abnormal social interaction persisted. These animals also experienced a gradual decline in hematocrit and hemoglobin values during the period of examination. Liver and kidney biopsies obtained from these lead-exposed animals revealed characteristic intranuclear inclusions.When adolescent and adult monkeys were exposed to doses of lead acetate similar to those employed in the infant experiments, lead levels in excess of 200 mug/100 ml of blood were recorded. However, there were no obvious behavioral abnormalities observed. There were, however, numerous lead inclusion bodies in kidney biopsy specimens from these animals. These data suggest that, like man, the infant nonhuman primate is much more susceptible to lead intoxication than is the adult. The clinical and behavioral changes recorded in these infant rhesus monkeys suggest their use as an experimental model to evaluate lead intoxication.


Phase-dependent lobopodial contact paralysis.


D-ribulose 1,5-diphosphate carboxylase from Rhodospirillum rubrum. II. Quaternary structure, composition, catalytic, and immunological properties.


D-ribulose 1,5-diphosphate carboxylase from Rhodospirillum rubrum. I. Levels, purification, and effects of metallic ions.


On the toxic proteins abrin and ricin. Studies of their binding to and entry into Ehrlich ascites cells.


A comparison of the L-arabinose- and D-galactose-binding proteins of Escherichia coli B-r.


Meningococcal conjunctivitis. Report of a case.


Development of knowledge of unity of tuberculosis and of the portals of entry of tubercle bacilli.


Purification and properties of flagellar outer doublet tubulin from Naegleria gruberi and a radioimmune assay for tubulin.


A method of freeze-drying and fixing bacterial cultures for electron microscopy.


Erroneous Model S Coulter Counter values on patients undergoing parenteral nutrition with intravenous lipid emulsions.


Problems in geriatrics.

Problems in geriatrics.


Nursing procedures: you'd be amazed at what they cost. (And maybe you're not to blame).


Cromolyn--metaproterenol.

Cromolyn--metaproterenol.


The effect of prostaglandin E2 on the rhesus monkey pupil.


Effects of sequential treatment with estradiol and PGF2alpha on the length of the primate menstrual cycle.


Platelet aggregation induced by procollagen.


Biosynthetic activities in normal and pathological platelet populations.


Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) deficiency: immunologic studies on the mutant enzyme.


Immunological studies of hypoxanthine-guanine phosphoribosyltransferase in Lesch-Nyhan syndrome.


Unstable HPRTase in subjects with abnormal urinary oxypurine excretion.


Human hypoxanthine-guanine phosphoribosyltransferase (HGPRT): purification and properties.


The enzymatic conversion of cis-(14C)phytofluene, trans-(14C)phytofluene, and trans-zeta-(14C)carotene to poly-cis-acyclic carotenes by a cell-free preparation of tangerine tomato fruit plastids.


Chemical modification studies of chloroplast membranes. Water oxidation inhibition by diazonium-benzenesulfonic acid.


tRNA methylases from HeLa cells: purification and properties of an adenine-1-methylase and a guanine-N2-methylase.


L-Rhamnulose 1-phosphate aldolase of Escherichia coli. The role of metal in enzyme structure.


Antigenic analysis of raw soybean: variation of host response during prolonged immunization.


Purification and characteristics of pyocin B39. I. General properties.


Isolation of Toxoplasma during unsuccessful attempts to isolate Rickettsiae from swine and rodents in Taiwan.


Bacterial meningitis: current status of vaccines against the meningococcus and hemophilus influenzae type B.


A review of brucellosis in the horse.

A review of brucellosis in the horse.


Editorial: Diet and diverticular disease.


Collaborative study of the determination of polychlorinated biphenyls in paperboard.


Manual microbiological turbidimetric analysis of chlortetracycline in feeds.


Direct chromatography of some N-methylcarbamate pesticides.


Confirmation of pesticide residue identity. VII. Solid matrix derivation procedure for the simultaneous confirmation of heptachlor and endrin residues in the presence of large quantities of polychlorinated biphenyls.


Colorimetric method for estimation of carbaryl and its residues on grains.


Determination of carbaryl in pesticide formulations and fertilizers by high-pressure liquid chromatography.


Human leukemia cell antigens: serologic characterization with xenoantisera.


Differential analysis of cyclocytidine, 1-beta-D-arabinofuranosylcytosine, and 1-beta-D-arabinofuranosyluracil by ultraviolet spectophotometry.


Human leukemia antigens: partial isolation and characterization.


Effect of Brucella abortus infection (vaccine strain 19BA) on Rauscher leukemia virus and L1210 leukemia in mice.


Effect of oral contraceptives on the mammary glands of Rhesus monkeys: a preliminary report. To determine the relationship of oral contraceptive use and breast cancer, 96 rhesus monkeys were administered either Enovid-E (2.5 mg norethynodrel and .1 mg mestranol) or Ovulen (1 mg ethynodiol diacetate and .1 mg mestranol) cyclically for  5 years at doses of 1, 10 and 50 times the human dose.  The animals' progress was compared with a control group of 32 monkeys.  General physical and mammary gland examinations were conducted before treatment and monthly thereafter.  During the 5 year study period none of the treated animals demonstrated clinical evidence of mammary gland lesions, no deaths from breast malignancy occurred, and no palpable breast nodules were found.


The problem of the alcohol-dependent individual.


Editorial: Plastic surgical treatment of basal cell skin cancer.


Improved therapy for Vietnam acquired vivax malaria.


Host factors in human frostbite.

Host factors in human frostbite.


A survey to assess potential human disease hazards along proposed sea level canal routes in Panamá and Colombia. V. Arbovirus infection in non-human vertebrates.


Serum uric acid and cholesterol variability for men aboard a Polaris submarine.


Medical evacuations for selected chronic diseases: USARPAC, 1970-71.


The effect of premedication and angiography on arterial blood gases.


Meningococcemia presenting as septic arthritis: case report.


Cerebral vasospasm: a review.

Cerebral vasospasm: a review.


The Alcohol Rehabilitation Unit, National Naval Medical Center, Bethesda.


Preparation of mono-specific antisera against staphylococcal alpha- and beta-hemolysin by using agar gel precipitates as immunogen.


Reserpine in experimental cerebral edema: further observations.


Recurrent pneumomediastinum in diabetic ketoacidosis.


Pesticide residues in total diet samples. VI.


Letter: Anticonvulsant therapy in cerebral injuries.


Temporally-related stimulus properties of delta9-tetrahydrocannabinol in monkeys.


Intrathoracic vagus nerve tumours.

Intrathoracic vagus nerve tumours.


The identification of rumen ciliates from eastern Bos taurus.


Histocompatibility-linked immune-response genes in the rhesus monkey.


Further studies of tissue-typing sera in baboons.


Beneficial influence of limited histocompatibility of bone marrow grafted to unrelated rhesus monkeys preconditioned with x-ray and ALS.


Further studies with HL-A reagents raised in primates: analysis of the human HL-A2-HL-A28 cross-reaction.


Isolation of baboon xenogeneic histocompatibility antigens.


Characterization of lymphocyte subpopulations of nonhuman primates separated by free-flow electrophoresis.


Use of nonhuman primates for assaying tumorigenicity of viral-vaccine cell substrates.


Immunopathogenesis of dengue infection in the rhesus monkey.


Transfer of delayed hypersensitivity to nonsensitive human leukocytes with rhesus-monkey lymphoid RNA extracts.


Studies on rhesus-monkey nondialyzable and dialyzable transfer factors.


Isolation of Theileria mutans from cattle in Essex.


Lassa fever and Marburg virus disease.

Lassa fever and Marburg virus disease.


Effect of barometric pressure change on the ear following stapedectomy.


Heat stability of the precipitin reactants in normal chicken sera.


Genetic control of urea resistant esterase of liver extracts in chicken.


Protein-bound sulfhydryl groups and thiolesters in mitochondria and submitochondrial particles and their relationships to oxidative phosphorylation.


Studies on the mechanism of action of nalidixic acid. With three independent techniques (absorption spectrophotometry, measurement of the deoxyribonucleic acid [DNA] melting temperature, and equilibrium dialysis), no evidence has been found for the binding of nalidixic acid to purified DNA. Also, no evidence has been found to support the hypothesis that nalidixic acid is permanently modified to a new, active compound by the bacterial cell. By using an in vitro DNA replication system developed by Bonhoeffer and colleagues, soluble extracts from nalidixic acid-sensitive cells have been shown to confer nalidixic acid sensitivity on the DNA synthesis of lysates from nalidixic acid-resistant cells. The activity in the extracts is only present in sensitive cells and is nondialyzable and heat sensitive. Finally, two known nalidixic acid-resistant mutants of Escherichia coli, mapping at nal A and nal B, respectively, have been tested to determine whether either of them is a transport mutant. It has been shown that nal B(r) is a transport mutant whereas nal A(r) is not.


Interaction of spinach leaf adenosine diphosphate glucose alpha-1,4-glucan alpha-4-glucosyl transferase and alpha-1,4-glucan, alpha-1,4-glucan-6-glycosyl transferase in synthesis of branched alpha-glucan.


Bacillus licheniformis 749-C plasma membrane penicillinase, a hydrophobic polar protein.


Studies on spinach leaf ribulosebisphosphate carboxylase. Carboxylase and oxygenase reaction examined by immunochemical methods.


Purification and properties of T2 bacteriophage-induced thymidylate synthetase.


Purification of rat urinary kallikreins and their specific antibody.


Topography of ribosomal proteins of the Escherichia coli 30S subunit as studied with the reversible cross-linking reagent methyl 4-mercaptobutyrimidate.


Platelet satellitism.

Platelet satellitism.


Responsiveness of human granulocytic leukemic cells to colony-stimulating factor.


The synaptinemal complex in homologous and nonhomologous pairing of chromosomes.


One the nature of chromosome-sized DNA molecules.


The units of DNA replication in Drosophila melanogaster chromosomes.


Organization and transcription of DNA in chromosomes and mitochondria of Drosophila.


Characterization of the rapidly renaturing sequences in the main CsCl density bands of Drosophila, mouse, and human DNA.


Bulk isolation of metaphase chromosomes from an in vitro cell line of Drosophila melanogaster.


Repetitive sequences in isolated Thomas circles from Drosophila melanogaster.


Repetitive DNA in polytene chromosomes of Drosophila melanogaster.


Unequal mitotic sister chromatid exchange and disproportionate replication as mechanisms regulating ribosomal RNA gene redundancy.


Studies on the transfer RNA genes of Drosophila.


An examination of the one cistron: one chromomere concept.


Analysis of a whole chromosome in Drosophila.


The one band-one gene hypothesis: evidence from a cytogenetic analysis of mutant and nonmutant rearrangement breadpoints in Drosophila melanogaster.


Organization of chromomeres.

Organization of chromomeres.


Chromosome structure in ciliated protozoans.


Temporal control of puffing activity in polytene chromosomes.


Transcription and chromatin structure.

Transcription and chromatin structure.


Ultrastructure of active eukaryotic genomes.


Experimental glaucoma in the rhesus monkey.


Lenticular and fundus changes induced by the intraocular infusion of sodium aspartate.


Some enzymic properties of human lens esterase I.


Intraocular pressure following long posterior ciliary canal cautery in primates.


The nature of chorioretinal lesions produced by the gallium arsenide laser.


Transient lack of thyrotropin (TSH) response to thyrotropin-releasing hormone (TRH) in treated hyperthyroid patients with normal or low serum thyroxine (T4) and triiodothyronine (T3).


Serum growth hormone and prolactin response to thyrotropin-releasing hormone in patients with acromegaly before and after surgery.


Parathyroid function in patients with hyper- or hypothyroidism.


Recovery of function: a review of studies pertaining to the serial lesion procedure and brain damage in infancy.


Letter: Effect of Rev 1 vaccination.

Letter: Effect of Rev 1 vaccination.


Assessment of bowel viability using 99Mtechnetium-tagged albumin microspheres.


Experimental evaluation of the "gastric barrier" in CSF shunt infections.


The study of bile salt kinetics in the experimental animal using a new technique.


Spontaneous diabetes in monkeys.

Spontaneous diabetes in monkeys.


Histopathology of the myocardium in 50 apparently healthy cats.


Clinical assessment of reproductive function in female rhesus monkeys: individual physical examination.


Lethal trait A 46 in cattle. Immunological investigations.


Editorial: Drugs in asthma.

Editorial: Drugs in asthma.


The changing role of occupational therapy.


Characterization of salivary proteins in patients with Sjögren's syndrome.


Micromelic type of spondylo-meta-epiphyseal dysplasia.


Biologic effects of dental materials. 3. Toxicity and antimicrobial effect of endodontic antiseptics in vitro.


The effect of products D and E on the thrombin induced conversion of fibrinogen to fibrin.


IgG subclasses: relationship to clinical aspects of multiple myeloma and frequency distribution among M-components.


Fine structure of rat incisor ameloblasts in transition between enamel secretion and maturation stages.


Red-cell carbonic anhydrase isoenyzmes in megaloblastic anaemia.


Characteristics of a nucleoproteinaceous subviral entity resulting from partial degradation of southern bean mosaic virus.


F116, D3 and G101: temperate bacteriophages of Pseudomonas aeruginosa.


effects of aldehyde fixation and of preganglionic denervation on acetylcholinesterase and butyrocholinesterase of cat autonomic ganglia.


Squirrel monkey platform runway test. A preliminary report.


The switch point in mu heavy chains of human IgM immunoglobulins.


Hydrogen-tritium exchange in polypeptides. Models of alpha-helical and beta conformations.


Isolation and properties of the principal liver protein conjugate of a hepatic carcinogen.


Kinetics of interaction of cyanate ion with cobalt bovine carbonic anhydrase.


Characterization of an additional protein in pancreatic juice of men with chronic calcifying pancreatitis. Identification to lactoferrin.


Anterior pharyngeal pouch.

Anterior pharyngeal pouch.


Three unusual foreign bodies.

Three unusual foreign bodies.


Microtubules and movement in the archigregarine, Selenidium fallax.


The purification and properties of a ribonuclease of the roe of the scallop Chlamys opercularis.


Protein synthesis in a cell-free preparation from rumen Protozoa.


Angiokeratoma corporis diffusum (Fabry's diseases).


Human pancreatic proteolytic enzymes and protein inhibitors. Isolation and molecular properties.


Can Aspergillus flavus cause maduromycetoma?


Selection for reduced crossing over in Drosophila melanogaster. Selection was practiced for reducing crossing over between the third chromosome genes Sb and H(2) of Drosophila melanogaster; the method employed was to select the repulsion double heterozygotes Sb+/+H(2) every generation. Two replicate selection lines were maintained. After 24 generations of selection, Line 1 showed no significant difference from the control, although the regression of recombination value on generation was significant. In generation 20, Line 2 had a significantly lower recombination value than the control, as well as having a highly significant regression coefficient. No chromosome rearrangements were involved in the response. It was concluded that there was substantial genic variability in the frequency of crossing over between Sb and H(2) in the base population.


On recombination-defective meiotic mutants in Drosophila melanogaster. The genetic effects of four recombination-defective meiotic mutants in D. melanogaster on recombination, segregation and the relationship between the two have been examined. The results suggest the following. (1) The anomalous meiotic segregation observed in females carrying recombination-defective meiotic mutants is a normal consequence of the reduction in exchange; each recombination-defective mutant can, therefore, be defined by a single lesion in the control of recombination. (2) Of the operations used to date to characterize this lesion, the most informative is whether the decrease in recombination is uniform along the chromosome arm or nonuniform; in particular, if the formation of recombinants is visualized as a two-step process consisting of the establishment of possible exchange points (exchange preconditions) followed by exchange itself, then mutants that uniformly decrease crossing over involve defects in the second step while mutants that result in a nonuniform decrease involve defects in the establishment of exchange preconditions. (3) Of the fourteen loci identified by recombination-defective meiotic mutants, only one (with two alleles) is involved in exchange itself; the others all reduce recombination most drastically in distal regions, suggesting that the establishment of exchange preconditions involves polar processes. (4) A very general description of the polar establishment of exchange preconditions is presented; this description has the property that if a precondition meiotic mutant affects interference, the coefficient of coincidence will be increased in proportion to the decrease in recombination which is what is observed for all recombination-defective meiotic mutants studied to date.


Genetic dissection of segregation distortion. I. Suicide combinations of SD genes. Two major loci in the Tft-cn region of an SD chromosome have been separated by recombination and identified. The allele at the left-hand locus on an SD chromosome is called Sd; the allele at the right-hand locus is called Rsp. Both Sd and Rsp are necessary to bring about a distortion of the segregation ratio in heterozygous SD males, although the particular degree of distortion exhibited by an SD chromosome is influenced by the constellation of polygenic modifiers of SD in the genome. The coupling phase of the alleles, Sd Rsp/Sd(+)Rsp(+), produces about 89-90% of Sd Resp-bearing progeny. The repulsion phase, Sd Rsp(+)/Sd(+)Rsp, produces 10-20% of Sd Rsp(+)-bearing progeny. No coupling-repulsion effects between Sd and Rsp are apparent.


A cold-sensitive zygotic lethal causing high frequencies of nondisjunction during meiosis I in Drosophila melanogaster females. The X-linked, cold-sensitive zygotic lethal, l(1)TW-6(cs), both in homozygous and heterozygous females, induces nondisjunction of all four chromosomes at Meiosis I at both 25 degrees and 17 degrees . Nondisjunction frequencies approaching 0.5 for the X and fourth chromosomes have been observed at 16 degrees -18 degrees . The disjunction of the X chromosomes in males is not affected. The mutant causes mitotic irregularities in zygotes at both 25 degrees and 17 degrees . Mortality of all zygotes produced by the crosses 6(cs)/6(cs)x6(cs)/B(s)Y and FM7/6(cs)x6(cs)/B(s)Y is respectively 86% and 67-74% at 25 degrees and 99.8-99.9% and 94% at 17 degrees . The mortality of 6(cs) hemizygotes derived from females carrying no doses of 6(cs) (C(1)DX,y f/yx6(cs)/B(s)Y) is 45-55% at 25 degrees and 98% at 17 degrees . The length of the temperature-sensitive period for 6(cs) homo- and hemizygotes is affected by the maternal dosage of 6(cs); the shortest TSP is for zero and the longest is for two maternal doses. Mortality takes place primarily during embryogenesis with some larval and little pupal mortality. Analysis of sectioned embryos indicates that the large array of different patterns of damage observed could have arisen from abnormal cleavage divisions and the incomplete population of the blastoderm with nuclei.


The developmental genetics of the temperature sensitive lethal allele of the suppressor of forked, 1(1)su(f)ts67g, in Drosophila melanogaster. A temperature-sensitive lethal allele of suppressor of forked, l(1)su(f)(ts67g) (ts67), has been discovered and characterized as follows: Flies which are hemizygous for ts67 live at 18 degrees and 25 degrees but die at 30 degrees primarily as larvae. The temperature-sensitive period for ts67 homozygotes or hemizygotes begins in second instar and ends at pupation. ts67 is lethal at 30 degrees when heterozygous with suppressor of forked (su(f)), a deficiency for suppressor of forked (su(f)(-)), and a non-conditional lethal allele of suppressor of forked (3DES). It is viable at 30 degrees when heterozygous with the wild-type allele of suppressor of forked. At 25 degrees but not at 18 degrees forked bristles are suppressed in flies of the following genotypes: f(s)ts67/Y, f(s)ts67/f(s)ts67, f(s)ts67/f(s)su(f), f(u)ts67/f(s)3DES, f(u)ts67/f(s)su(f)(-), f(u)ts67/f(s)su(f). There is some suppression of forked bristles at 25 degrees in the heterozygote, f(s)ts67/f(s)+(su(f)). The forked bristle phenotype is not suppressed at either temperature in flies of the genotypes f(u)ts67/Y, f(u)ts67/f(u)ts67/ (f(s) and f(u) indicating suppressible and unsuppressible alleles of forked). The temperature-sensitive period for suppression of forked bristles begins at pupation and extends through the period of bristle synthesis. The deficiency phenotype (bristles reduced in size or absent, wing wrinkled or blistered, eyes rough) typical of flies of the genotype f(s)su(f)/f(s)su(f)(-) at 18 degrees and 25 degrees , is exhibited by flies of the genotypes f(s)ts67/f(s)su(f)(-) at 25 degrees and f(u)ts67/f(s)su(f) at 29 degrees . An allele of lozenge (lz(1)) which can be suppressed by su(f) is suppressed at 25 degrees but not at 18 degrees in lz(1)ts67/Y males. ts67 homozygous females are fertile at 25 degrees but sterile at 30 degrees . The hypothesis is discussed that the su(f) locus codes for a ribosomal protein and that suppression and enhancement are affected by mutations at the locus by mutant ribosome-induced misreading. The possibility is presented that ts67 may be used to determine the translation time in development of any gene.


The effects of r- and K-selection on components of variance for two quantitative traits. The genetic and environmental components of variance for two quantitative characters were measured in the descendants of Drosophila melanogaster populations which had been grown for several generations at densities of 100, 200, 300, and 400 eggs per vial. Populations subject to intermediate densities had a greater proportion of phenotypic variance available for selection than populations from either extreme. Selection on either character would be least effective under pure r-selection, a frequent attribute of selection programs.


The distribution of mucins in the nasopharynx of some primates.


Development of the secondary interstitial gland in the rabbit ovary.


Proceedings: The pharyngeal hypophysis in the laboratory animal.


Proceedings: Immunoglobulin in pig salivary gland homogenates.


Proceedings: Gene frequency variation in Kenya baboon populations.


Hearing in the owl monkey (Aotus trivirgatus). I. Auditory sensitivity.


Thermal thresholds of the rhesus monkey (Macaca mulatta).


Ovarian hormones and sexual behavior in Macaca Nemestrina.


The effect of enzymes on structural and biological properties of Semliki forest virus.


Effect of fragmentation on interferon induction by double-stranded virus RNA.


Tissue fixation with diimidoesters as an alternative to aldehydes. I. Comparison of cross-linking and ultrastructure obtained with dimethylsuberimidate and glutaraldehyde.


Amodioquine induced oral pigmentation--a light and electron microscopic study.


The effects of bacterial plaque extracts on organ cultures of fetal rat masticatory mucosa. I. Histochemistry.


The effects of bacterial plaque extracts on organ cultures of fetal rat masticatory mucosa. II. Electron microscopy.


The crypt surface of blade-vent implants in clinical failure: an electron microscopic study.


Translation of Pseudomonas aeruginosa bacteriophage PP7 RNA by a cell-free amino acid incorporating system from Escherichia coli. We have compared the activities of the RNA genomes of Pseudomonas aeruginosa phage PP7 and coliphages Qbeta and f2 in a cell-free amino acid incorporating system derived from Escherichia coli. The rate of incorporation of [(14)C]leucine in the PP7 RNA-directed system is greater than in the systems directed by either Qbeta or f2 RNA. The response to changes in phage RNA concentrations is similar in all the systems, reaching a saturation level at 0.75 to 1.0 mg of RNA per ml of reaction mixture. Analysis of complete reaction mixtures of the PP7 RNA and of the Qbeta RNA systems by sucrose gradient centrifugation shows generally similar patterns for both RNAs. The principal differences are that in the PP7 system a slightly higher percentage of RNA forms ribosome complexes and that the polysomes are somewhat smaller. PP7 RNA is also degraded more extensively during the reaction than is Qbeta RNA. Analysis of the products of the reactions by acrylamide gel electrophoresis shows that PP7 coat protein is the only identifiable product of the PP7 RNA-directed system, suggesting that only the coat protein cistron is translated by E. coli ribosomes.


Ribosomal protein neighborhoods. 3. Cooperativity of assembly.


Strains of Bacillus subtilis synthesizing elevated levels of isoleucine-valine biosynthetic enzymes.


Differentiation of the contractile effects of prostaglandin E2 and the C-terminal octapeptide of cholecystokinin in isolated guinea-pig gallbladder.


The demonstration of antibodies to Sarcocystis fusiformis antigen in sera of Isospora hominis carriers, using the indirect fluorescence technique.


Biliary excretion of some penicillins, quaternary ammonium compounds and tertiary amines, including aspects on mechanisms for their excretion.


The indirect immunoenzyme test for the detection of antibodies against North American Borreliae. I. Technic and results with sera of vervet monkeys (Cercopithecus aethiops).


Efficacy of the Candida precipitin test. Verification with a protoplast antigen preparation.


Rapid detection of bacteremia by a radiometric system. A clinical evaluation.


Parenteral nutrition in seriously ill neonates. Search for the ideal mixture.


Serum proteins in hepatosis of pregnancy and pre-eclampsia.


Pigmenturia from conga drumming. Hemoglobinuria and myoglobinuria.


Care of the aged: an English lesson?

Care of the aged: an English lesson?


Editorial: How clinically useful is T and B cell quantitation?


Autonomic drugs in ophthalmology: some problems and promises. I. Directly and indirectly acting parasympathomimetic drugs.


Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. A new cephalosporin with a highly reactive beta-lactam ring was found to give an immediate color change in the presence of beta-lactamases from many bacteria, including staphylococci, Bacillus species, Enterobacteriaceae, and Pseudomonas. The reaction is confined to organisms producing beta-lactamases, but it is sufficiently sensitive to indicate the presence of this enzyme is small amounts in strains previously considered not to produce it. The compound has an unusual ultraviolet spectrum, and the color change can be followed quantitatively by measuring changes in absorption which occur in the 380- to 500-nm region, where cephalosporins normally have no absorption. The development of color is thought to be a consequence of the beta-lactam ring being unusually highly conjugated with the 3-substituent. Although in the bacteria only beta-lactamases produce this color change, it was found that serum and tissues from experimental animals also rapidly produced the colored breakdown product, which was then excreted in the urine. The mechanism of the mammalian breakdown was considered to be different from that found in bacteria.


Evaluation of a new cephalosporin antibiotic, cephapirin. Cephapirin sodium, a parenterally administered derivative of cephalosporanic acid, was tested in vitro against 150 stock cultures of Enterobacteriaceae and 30 stock cultures each of Pseudomonas aeruginosa and Staphylococcus aureus. Both broth- and agar-dilution techniques were employed with two sizes of inocula of organisms. At a concentration of 7.5 mug or less/ml, cephapirin inhibited and killed 100% of strains of Escherichia coli and Proteus mirabilis and more than 80% of Klebsiella species when tested against an inoculum of 10(5) bacterial cells/ml. However, even at 100 mug/ml, only a few isolates of other Enterobacteriaceae and Pseudomonas were inhibited. A 100-fold increase in the inoculum resulted in decreased susceptibility of organisms. All penicillin-susceptible as well as penicillin-resistant S. aureus isolates were inhibited and killed by 5 mug or less of cephapirin/ml when tested with an inoculum of either 10(4) or 10(6) organisms/ml. The drug also was studied in various doses in the treatment of 77 patients with diverse infections. Cephapirin was effective in the treatment of 27 of 32 patients with pulmonary infection, as well as in 6 of 7 patients with staphylococcal or streptococcal soft tissue infection. Of 25 patients with urinary-tract infections, 19 developed a negative culture during therapy. A single 4-g intramuscular dose of cephapirin was effective in only 2 of 11 patients with gonococcal urethritis or endocervicitis. Two patients with gonococcal urethritis treated with multiple injections were cured. The drug was well tolerated except for pain at the site of injection in 14 patients and phlebitis in 4 patients. No abnormalities in renal or hepatic function could be attributed to cephapirin. In addition, no abnormalities were found in the renal tubules of rabbits challenged with 500 mg of cephapirin/kg. If further studies document that cephapirin is well tolerated by the parenteral route, it may have advantages over cephalothin or cephaloridine.


Effect of exogenous penicillin on penicillin biosynthesis. The addition of phenoxymethylpenicillin (10 mg/ml) at any time during the penicillin fermentation inhibited further accumulation of the antibiotic in broth but had no effect on growth. Benzylpenicillin, 6-aminopenicillanic acid (6-APA), and some semisynthetic penicillins also showed this effect, but penicillin N, penicilloic acid, cephalosporin C, and 7-aminocephalosporanic acid did not limit penicillin accretion. Incorporation of radioactive precursors (cysteine, valine, and sodium phenoxyacetate) into penicillin in the presence of inhibitory concentrations of the antibiotic indicated that penicillin synthesis continued despite the lack of accretion of the antibiotic in broth. The rates of penicillin synthesis in a 48-hr and a 136-hr culture were compared by short-term exposure to Na(2) (35)SO(4), and no significant difference in the biosynthetic rate was observed. Exogenous penicillin in the range of 1 to 15 mg/ml of culture broth had no effect on utilization of acetate or glucose by Penicillium chrysogenum. The antibiotic-synthesizing capacity of the organism was not irreversibly inhibited by exogenous penicillin. The degradation of penicillin during the fermentation was also studied. Penicillin V was stable in broth filtrate. Catabolic enzymes such as penicillinase and penicillin-acylase were not demonstrated in whole broth, nor was the accumulation of 6-APA, penicilloic acid, or other degradation products detected. An examination of the intracellular penicillin concentration and the amount of penicillin associated with the mycelium revealed that cells contained significantly more penicillin late in the fermentation than earlier in the cycle. This cell-associated antibiotic may be a regulatory factor in further penicillin synthesis.


Factors influencing the microbiological assay of tobramycin. Tobramycin belongs to the aminoglycoside class of antibiotics. Assay procedures were investigated to determine the most suitable methods for the assay of tobramycin in blood, urine, and pharmaceutical preparations. The agar-plate assay was the method of choice for determinations in urine and serum. The turbidimetric assay was selected for the assay of pharmaceutical preparations. An extraction technique is described that minimizes problems encountered in the estimation of tobramycin in urine. Sample diluents, pH, and molarity were important and were variable depending on assay methods. Evidence is presented to show binding of tobramycin to alpha-cellulose susceptibility discs. Iron and sodium ions demonstrated potential inhibition of tobramycin in all assay methods tested.


Effect of chemotherapeutic agents upon microorganisms isolated from cancer patients. Attempting to explain the predominance of Pseudomonas in leukemic patients, five of the most common gram-negative organisms isolated from sites of infection in cancer patients were exposed to several of the chemotherapeutic agents used in the treatment of this disease (methotrexate, cytosine arabinoside, cyclophosphamide, and 6-mercaptopurine). At concentrations of 125 mug/ml or higher, methotrexate inhibited all organisms except Pseudomonas. Cytosine arabinoside inhibited Escherichia and Klebsiella but appeared to stimulate the growth of Pseudomonas slightly at the higher concentrations. Thus, significant differences existed in individual susceptibilities to these agents. Clinical isolates were more resistant than the corresponding laboratory strains not previously exposed to these compounds. The resistance of Escherichia coli to cyclophosphamide was decreased 26% when it was grown in mixed culture with Pseudomonas. Only Pseudomonas was resistant to all of these compounds whether in pure or mixed culture. These observations may help to explain, in part, the predominant role that Pseudomonas plays as an infectious agent in leukemic patients.


Scanning electron microscopy of the H37Ra strain of Mycobacterium tuberculosis exposed to isoniazid. The morphology of cells of the H37Ra strain of Mycobacterium tuberculosis exposed to 0.5 mug of isonicotinic acid hydrazide (isoniazid) per ml was examined by scanning electron microscopy (SEM). Cells that were exposed to isoniazid for 3 h showed no detectable change, whereas cells exposed to the drug for 24 h exhibited diverse morphological features. From our examination of these SEM pictures, we have reconstructed the probable sequence of morphological changes to be as follows: (i) the wrinkling of the cell surface was ascribed as the earliest observable change, (ii) the cell surface then became very rough and ragged, (iii) eventually the cytoplasmic material was extruded from the cell, (iv) this event produced a collapsed cell, (v) the cells began to fragment, (vi) the fragmented cells then coalesced to form an amorphous mass of cell debris.


Bactericidal action of an antibiotic produced by Myxococcus xanthus. Myxococcus xanthus produced an antibiotic during the end of its exponential growth phase which was capable of inhibiting growth of several gram-positive and gram-negative bacteria. The antibiotic was bactericidal to growing cultures only; chloramphenicol inhibited the bactericidal action of the antibiotic. Upon addition of the antibiotic to Escherichia coli B, deoxyribonucleic acid and ribonucleic acid as well as turbidity of the culture continued to increase even after the viable count decreased; the culture lysed about 60 min after addition of sufficient concentrations of the antibiotic. Spheroplasts could be prepared if the antibiotic was added to a culture growing in the presence of high concentrations of sucrose and MgSO(4). Mutants of M. xanthus FB which are incapable of fruiting body formation or glycerol-induced myxospore formation also produced the antibiotic. A mutant of E. coli resistant to the purified antibiotic was isolated in order to study the role of the antibiotic in the predatory behavior of myxococci.


Streptococcal pharyngitis therapy: comparison of clindamycin palmitate and potassium phenoxymethyl penicillin. Clindamycin palmitate and potassium phenoxymethyl penicillin were evaluated in 103 children with upper respiratory illnesses and pharyngeal group A streptococci, from November 1970 to July 1971. The children were assigned randomly by weight to one of the antibiotic regimens given orally for 10 days. Clindamycin palmitate and potassium phenoxymethyl penicillin dosages were 75 and 125 mg, respectively, in 5 ml tid for children weighing less than 25 kg, and 150 and 250 mg, respectively, in 10 ml bid for children weighing 25 kg or more. Recurrences of the original streptococcal group A, M, and T types within 3 weeks after the end of treatment were classified as failures. The failure rates were: clindamycin palmitate, 10% (5 of 52), and potassium phenoxymethyl penicillin, 18% (9 of 51). Possible drug-related rashes were observed in 8 of 52 clindamycin palmitate-treated patients. The geometric mean minimal inhibitory concentrations of clindamycin and penicillin against 103 isolates of group A streptococci were 0.033 and 0.007 mug/ml, respectively. The serum concentrations about 70 min after ingesting 150 mg of clindamycin palmitate averaged 3.8 mug/ml and after 250 mg of potassium phenoxymethyl penicillin averaged 0.9 mug/ml. Clindamycin palmitate was as effective as potassium phenoxymethyl penicillin in eradicating group A streptococci from the pharynx in tid and bid regimens. Nevertheless, because of its rash-producing tendency in some patients and higher cost, clindamycin palmitate should not be preferred to penicillin for treatment of streptococcal sore throat in the non-penicillin-allergic patient.


Letter: Chronic brucellosis.

Letter: Chronic brucellosis.


Letter: Treatment of meningococcal carriers.


Variation in the occurrence of extracellular diffusible antigens in temperature-induced variants of Erwinia herbicola Y46, and observations of their relationships with Erwinia amylovora.


The in vitro degradation of the herbicide bromoxynil.


Endotracheally administered gentamicin for the prevention of infections of the respiratory tract in patients with tracheostomy: a double-blind study.


Australia hepatitis-associated antigen in cirrhosis of the liver at Aurangabad.


Haemagglutination by Chikungunya virus--pH range and erythrocytes of different animals.


Nematodes in rhesus monkeys.

Nematodes in rhesus monkeys.


Kinetics of formation and dissociation of manganese-bovine carbonic anhydrase B.


The relationship between PF-DIL and activated human Hageman factor.


Hypothalamic regulatory hormones: a review.


IgM-forming cells as the immediate precursor of IgA-producing cells during ontogeny of the immunoglobulin-producing system of the chicken.


Dissociation and recombination of the subunits of the cholera enterotoxin (choleragen).


Chain interactions and idiotypic specificities of homogeneous rabbit anti-lactose antibodies.


Effector mechanisms of cell-mediated immunity to xenogeneic cell antigens.


Interaction of isoantibody and cytotoxic lymphocytes with allogeneic tumor cells.


Modification of NZB-NZW F1 autoimmune disease by development of tolerance to DNA.


Crossed immunoelectrophoresis and electroimmunoassay of IgG.


The occurrence of shared idiotypic specificity among the goat antibodies that distinguish human hemoglobin S from A.


Serum immunoglobulin E levels in patients with neoplastic disease.


Studies on human secretory immunoglobulin A. VI. Cyanogen bromide cleavage.


Prevention of myeloma growth in BALB-c mice by injection of anti-immunoglobulin antisera.


Interaction of C-reactive protein complexes with the complement system. II. Consumption of guinea pig complement by CRP complexes: requirement for human C1q.


The diagnosis of ruptured fetal membranes by measurement of the diamine oxidase (DAO) activity in vaginal fluid.


Letter: Alcoholic cirrhosis in baboons.

Letter: Alcoholic cirrhosis in baboons.


Comparative studies of alpha-lactalbumin and lysozyme: Echidna lysozyme.


Effects of dihydrotestosterone on sexual behavior of castrated male rhesus monkeys.


A study of the histologic reaction of submerged root segments.


Schedule-induced drinking following omission of reinforcement in the rhesus monkey.


Normal physiological patterns and physiological-behavioral correlations in unrestrained monkey infants.


Correlation of physical characteristics of 4 MV X-ray beams with skin reactions of patients undergoing radiation therapy.


Irradiation in the local control of malignant lymphoreticular tumors (non-Hodgkin's malignant lymphoma).


Functional and histological findings in acoustic tumor.


Mechanisms of renal excretion of urate in the rat.


Tuberculous cervical lymphadenitis.

Tuberculous cervical lymphadenitis.


Methionine transfer RNAs from the blue-green alga Anacystis nidulans.


Proceedings: The intracranial volume-pressure response during experimental brain compression in primates.


Cardiolipin specific phospholipase D activity in Escherichia coli extracts.


Peroperative evaluation of the viability of ischaemic colon in primates.


Proceedings: Comparison of central venous pressure with continuous blood volume measurement during haemorrhage and infusion.


Editorial: Budget cuts are not the answer.


Neutralizing and precipitating antibodies against infectious bronchitis virus in chickens.


Classification of hypothyroidism in evaluating patients after radioiodine therapy by serum cholesterol, T3-uptake. Total T4, FT4-index, total T3, basal TSH and TRH-test.


Basement membrane abnormalities in melanotic tumor formation of Drosophila.


Intramitochondrial bodies in the B-cells of rat's pancreas treated by glybenclamide.


Antibodies to the insect moulting hormone beta-ecdysone.


Review of progress and application in acoustis microscopy.


Australia antigen in the Solomon Islands.


Fine structure and function of ocular tissues. The optic nerve.


The dorsal thalamus of the cat and comparison with monkey and man.


Products of linoleic hydroperoxide-decomposing enzyme of alfalfa seed. Alfalfa seeds and seedlings contain an enzyme that catalyzes a reaction with the 13- and 9-hydroperoxides of linoleic acid to form 13-hydroxy-10-oxo-trans-octadecenoic acid and 9-hydroxy-12-oxo-trans-10-octadecenoic acid, respectively. When commercial lipoxygenase is used to generate the hydroperoxides, the above acids appear in about 2:1 proportions, respectively. The products of the action of the enzyme on the hydroperoxides were stabilized for analysis by reduction with H(2) and LiAIH(4). Trimethylsilyl derivatives of reduced products were analyzed by combined gas-liquid chromatography-mass spectrometry. Specific deuterium labeling permitted definite location of the oxo functions. (18)O(2) labeling experiments showed that the oxygens of both the oxo and the hydroxyl functions were derived from the hydroperoxide. Retention of both oxygens suggests that the reaction proceeds through a cyclic epiperoxide followed by a ketohydroxy-forming rearrangement. No products of hydroperoxide isomerase were found in reactions catalyzed by the crude enzyme from alfalfa seeds.


Cholesterol metabolism in rhesus monkey, squirrel monkey, and baboon. The metabolism of cholesterol was studied in baboons, rhesus monkeys, and squirrel monkeys while they were being fed either a low fat, low cholesterol (basal) diet or the basal diet supplemented with saturated fat and cholesterol (atherogenic diet). When the diet was changed from basal to atherogenic, the mean total serum cholesterol concentration increased from 70 to 180 mg/dl in the baboon, from 168 to 283 mg/dl in the squirrel monkey, and from 144 to 608 mg/dl in the rhesus monkey. In animals fed the atherogenic diet, the percentage of dietary cholesterol absorbed was greatest in the rhesus monkey and least in the baboon. The fraction of the total body pool of cholesterol that was derived from the diet was greatest in the squirrel monkey and least in the baboon. The turnover of the body pool of cholesterol was several times faster in the squirrel monkey than in the baboon or the rhesus monkey when either dict was fed. The mean total fecal excretion of endogenous cholesterol and bile acid increased in all species on transition to the atherogenic diet; however, the relative contributions of the neutral and acidic fractions to the increase in total excretion differed among species. The difference in percentage of dietary cholesterol absorbed may, in part, account for the large differences in serum cholesterol during the atherogenic diet period. Comparison with other published results indicates that of these species cholesterol metabolism in the baboon is most like that in the human.


Artifacts produced by acidic hydrolysis of lipids containing 3-hydroxyalkanoic acids. After acidic hydrolysis of lipid A preparations from pseudomonads, products containing both hydroxy and nonhydroxy fatty acids were obtained. The major products were alkanoate esters of the hydroxy acids. Similar compounds were formed when a 3-hydroxy acid was heated with a nonhydroxy acid under the same conditions.


Anti-C, anti-e and anti-Ce (rhi) as a cause of hemolytic disease of the newborn in a Thai family.


The need for trained medical professionals.


Drug rehabilitation in the combat zone.

Drug rehabilitation in the combat zone.


Categorization of seizure disorders in combat zone evacuees.


Case for diagnosis.

Case for diagnosis.


Home dialysis experience at Madigan Army Medical Center.


Methoxyflurane nephrotoxicity: case report.


Pulpal response of monkeys to a composite resin cement.


Donnatal intoxication: case report.

Donnatal intoxication: case report.


Delayed infection following tantalum cranioplasty: case report.


Subarachnoid hemorrhage presenting as cardiac arrhythmia: case report.


Editorial: oral fluid intake accuracy.

Editorial: oral fluid intake accuracy.


Progressive ossification of the leptomeninges in atrophic encephalopathy.


Ion exchange and affinity chromatography during the purification of human plasminogen on sepharose-L-lysine.


Did this boy have meningitis?

Did this boy have meningitis?


Eight children with coexistent sickle cell anemia and plumbism.


Candida endocarditis and encephalitis in an infant.


Candida endocarditis complicating glucose total intravenous nutrition. A case of Candida albicans endocarditis is reported. The endocarditis occurred in a patient with a chronic illness who received intermittent glucose total intravenous nutrition for approximately 10 weeks. The patient developed severe aortic insufficiency with his valvular endocarditis and required emergency aortic valve replacement. Aggressive surgery and medical treatment appear to be the treatment of choice for these critically ill patients.


Novel human serum protein from fibroblast plasma membrane.


Review: The endocrine glands in infantile malnutrition.


Oxidation of phenols by cells and cell-free enzymes from Candida tropicalis.


Proceedings: Immunoglobulins in early syphilis.


The influence of repeated and prolonged stimulation on the PHA-response of lymphocytes in Hodgkin's disease.


Enhanced uptake of anti-Rho coated red cells by cultured human monocytes.


Lysozyme-negative, peroxidase-positive mononuclear cells: a new kinetically distinct cell population in normal rat blood.


Splenic feedback in red cell regeneration.


'Nonsecretory' multiple myeloma. Report of a case.


Effect of glucose on the biosynthesis of the membranes of Bacillus.


Grey scale echography in the diagnosis of intrahepatic disease.


Ultrasound studies in premature infants after birth.


Letter: Fetal biparietal diameters correlated with gestational age.


Effects of ions on vesicles and phospholipid dispersions studied by polarization of fluorescence.


Proceedings: Studies on glucuronic acid metabolism: effect of uridine diphosphate glucuronic acid and thiol groups on the enzymes in the glucuronic acid metabolism series.


Proceedings: Possible mechanism of action of methyldopa: relationship studies between route on administration, dose, plasma concentration and hemodynamic changes in conscious monkeys.


Proceedings: Studies on drug dependence report. 19: quantitative evaluation of reinforcing effect on drug-seeking behavior.


Epidemiologic aspects of nontuberculous mycobacterial disease and of tuberculosis in British Columbia.


Evidence for two immunologically distinct acetyl-co-enzyme A synthetase in yeast.


Effect of photooxidation of bacterial liquefying alpha-amylase dependent on the degree of polymerization of linear substrates.


UV irradiation of poplar cells of Drosophila melanogaster embryos. V. A study of the meiotic recombination in females with chromosomes of different structure.


Degradation of tuberculoproteins by cathepsin D-type proteinase of alveolar and peritoneal macrophages of mice.


Population genetics in the American Tropics. IX. Rhythmic genetic changes that prove the adaptive nature of the detrimental load in Drosophila melanogaster from Caracolisito, Colombia.


In vitro activity of cephalosporins against Mycobacterium tuberculosis H37Rv: structure-activity relationships.


Metabolism of lysolecithin in vivo and in vitro with particular emphasis on the arterial wall.


Slime production by Pseudomonas aeruginosa. IV. Chemical analysis of two varieties of slime produced by Pseudomonas aeruginosa.


Stability of frozen solutions of cephapirin sodium.


Diethylpropion hydrochloride (Tenuate Dospan) in combination with hypotensive agents in the treatment of obesity associated with hypertension.


Tobramycin therapy of pulmonary infections in patients with cystic fibrosis.


Clinical experience with doxycycline in the treatment of surgical soft-tissue infections.


Treatment of otitis externa: a controlled bacteriological-clinical evaluation.


Prevention of swimmer's ear by simple prophylactic regimen.


Tolmetin versus placebo for the treatment of rheumatoid arthritis: a sequential double-blind clinical trial.


Doxepin versus amitriptyline in depression: a sequential double-blind study.


Effect of CDP-choline in experimental cerebral hemorrhage.


An early clinical trial of lenperone (AHR 2277), a butyrophenone, in chronic schizophrenia.


Antibiotic disk susceptibility tests with Neisseria gonorrhoeae. We have used a standardized disk diffusion procedure to test the susceptibility of 892 isolates of Neisseria gonorrhoeae to six antibiotics. The disk diffusion test described is a modification of the disk test of Bauer, Kirby, Sherris, and Turck, which is widely used for susceptibility testing of fast-growing, aerobic pathogens. With all six antibiotics (penicillin, ampicillin, tetracycline, erythromycin, chloramphenicol, and cephaloridine), disk test results were found to be reproducible and correlated well with the minimal inhibitory concentration as determined by an agar dilution procedure. The coefficients of correlation ranged from -0.79 with cephaloridine to -0.93 with penicillin. The technique described for disk susceptibility testing of gonococci can be used both for research and clinical purposes.


Semiautomated turbidimetric microbiological assay for determination of cefazolin. The Autoturb System, a semiautomated system for photometric bioassay, was used to determine cefazolin content. Suitable conditions for the assay using Streptococcus faecalis ATCC 10541 as the indicator organism included a medium pH of 6.0 to 7.0 and an incubation time of 3 to 3.5 h at 36 C. Multiple independent assays of samples from a common batch showed the test to be highly reproducible. Accuracy of the turbidimetric assay was evaluated by comparing data obtained from a chemical (hydroxylamine) and a biological (disk diffusion) assay. The available data show the turbidimetric assay to be a rapid, accurate, and reproducible method for determining the biological activity of cefazolin samples.


Dissociation between results of in vitro and in vivo antibiotic susceptibility tests for some strains of Pseudomonas aeruginosa. The relative efficacy in vivo of the polymyxin and aminoglycoside antibiotics in experimental Pseudomonas infection has apparently never been established. This study was designed to determine such efficacy in experimental infection of mice. Eleven strains of Pseudomonas aeruginosa were selected for the study, representing six of the seven recognized immunotypes. All strains were susceptible to colistin, gentamicin, and tobramycin in vitro, and all were virulent for mice. None was lethal because of preformed toxins. Infections were established in mice by intraperitoneal (i.p.) injection of 100 ID(50) of bacteria (infectious doses that would kill 50% of the mice). Graduated doses of antibiotics or saline were given subcutaneously 1 h later. Infections by three strains of P. aeruginosa were cured by low doses of all three antibiotics. Infections by two strains were relatively resistant in vivo to all three antibiotics. Infections by seven strains were relatively resistant in vivo to colistimethate. For most strains, colistimethate was less effective in vivo than gentamicin or tobramycin. To determine the influence of the size of the bacterial inoculum upon the results of chemotherapy, groups of mice were infected with serial dilutions of bacterial suspensions and treated with antibiotics. The antibiotics were more effective when a lighter bacterial inoculum was used. However, colistimethate at 30 mg/kg failed to cure some infections established with an inoculum of only 10 ID(50). To examine the possibility that death despite apparently adequate chemotherapy might be due to bacterial lysis and delayed release of toxins, numbers of viable bacteria in the peritoneal cavity of treated animals were determined by colony count. Antibiotic failure by colistimethate was associated with a steady rise in the number of i.p. bacteria. The findings support the conclusion that i.p. infections in mice with some strains of P. aeruginosa that are fully susceptible in vitro are relatively resistant to chemotherapy in vivo. The dissociation between in vitro and in vivo results was greatest for colistimethate.


Selective phosphorylation of the 5''-hydroxy group of ribostamycin by a new enzyme from Pseudomonas aeruginosa. It was found that the 5''-hydroxy group of ribostamycin was preferentially phosphorylated by a cell-free extract from Pseudomonas aeruginosa GN 573, a clinical isolate.


Biological assay of streptonigrin (NSC 45383) in body fluids and tissues of mice. Streptonigrin, a quinone antitumor antibiotic, has been reported to be effective in human trials. A sensitive and precise microbiological assay for the determination of distribution and concentrations of streptonigrin in the body fluids and tissues of treated mice has been developed in an attempt to supplement successful clinical application of this drug.


Ecology of Simuliidae, Mermithidae, and Microsporida in Newfoundland freshwaters.


Diabetic ketosis.

Diabetic ketosis.


The effect of extracellualr calcium on thermal excitability of the sensory units in the tooth of the cat.


Letter: Minimal effective dosage of cromolyn.


Letter: Families with high serum enzyme levels.


Editorial: Slow virus infections.

Editorial: Slow virus infections.


Loss of visual suppression of vestibular nystagmus after flocculus lesions.


Idiopathic membrano-proliferative glomerulonephritis in children (corrections between light, electron, immunofluorescent microscopic appearances and serum C3 and C4 levels).


Fine structure of Haemoproteus columbae Kruse during differentiation of the ookinete.


Fine structure of differentiating oocysts and mature sporozoites of Haemoproteus metchnikovi in its intermediate host Chrysops callidus.


A study on procaine and procaine adrenaline injections of the British Pharmacopoeia 1968.


Phosphorus regeneration in fresh-water paramecia.


The occurrence of vitellogenin in workers and queens of Apis mellifica and the possibility of its transmission to the queen.


Beta-alanine and adaptation in Drosophila.


Precipitation of myelin basic protein by beta-lipoprotein of human serum. A study of similarities and differences of the precipitation in normal sera and sera from patients with multiple sclerosis.


Cellular patterns of differing circadian rhythms and levels of RNA synthesis in Drosophila salivary glands.


Antigenic reactivity of inactivated Bacillus subtilis enzyme preparations.


Serum IgG, IgA, IgM, and IgE levels and allergy in Filipino children in the United States.


Photochemistry of bioactive compounds: photoproducts and kinetics of polychlorinated biphenyls.


The effect of changes of dietary calcium concentration on calcium metabolism in sheep.


Studies on a smooth phage-resistant variant of Brucella abortus. I. Immunological properties.


Body retention of injected 67Cu in patients with homozygous or heterozygous Wilson's disease and in normal subjects. Comparison of whole-body counting systems.


Localization of thyrotropin-releasing factor (TRF) in the hypothalamus of the rat.


Physicochemical appraisal of changes in egg white during storage.


Cell survival and architectonic differentiation of the hypothalamic mamillary region of the newborn mouse in culture.


Kinetics of thyroxine and triiodothyronine in a case of thyroxine-binding globulin deficiency with hypothyroidism.


Isolation and characterization of soluble and particulate thyroid iodoproteins in human congenital goiter.


Fractionated action of ethylenimine on the rate of mutation in Drosophila melanogaster.


Proceedings: Pharmacological studies on polychlorobiphenyl (PCB). 3. Effect on the content and metabolism of vitamin B2 and B6, and ascorbic acid in various organs of the rat.


Major urinary protein and immunoglobulin allotypes of recombinant inbred mouse strains.


Health problems and medical care in Papua New Guinea.


Sex-linked auxotrophic and putative auxotrophic mutants of Drosophila melanogaster. Thirty-two mutants with improved growth response on a yeast-sucrose compared with a defined medium have been characterized with respect to ribonucleoside supplementability. Twenty mutants respond to either pyrimidine ribonucleoside. Four mutants respond to one or both purine ribonucleosides. Eight mutants ("putative" auxotrophs) do not respond to dietary RNA supplementation. Mapping and complementation studies suggest that eleven loci are represented: one, rudimentary, probably accounts for all pyrimidine requirers; there are three purine loci and seven at which the putative auxotrophs are found.


Proceedings: Long-term toxicity study of methylmercuric chloride in monkeys.


The impact of chemotherapy on the care of patients with tuberculosis.


Development in genetic mosaics of aristapedia, a homoeotic mutant of Drosophila melanogaster. Development of the homoeotic mutation, aristapedia (ss(a)), was investigated by means of genetic mosaics. The wild-type alleles of aristapedia and the bristle markers yellow, singed, and forked were removed from cells at different times in development by X-ray induced somatic crossing-over. The phenotype of the resulting clones was examined in order to ascertain whether it was leg or antenna. The y sn f; ss(a) clones showed a leg phenotype if induced before the mid-third instar, but showed an antennal phenotype if induced after this time. Late non-expression of ss(a) may be due either to an influence of surrounding ss(+) tissues on the small ss(a) clones, or to a persistence of the effect of ss(+) for one or two cell generations after it is removed from a cell line.


Congenital lactose intolerance of gastrogen origin associated with cataracts.


Chemistry of wheat proteins and the nature of the damaging substances.


Aflatoxins in foods and feedstuffs. 3. Fluorodenisitometric method for determination of aflatoxins M1 and M2 in presence of aflatoxins B1, B2, G1, and G21.


Anthyridine and 1,10,11,12-tetraazanaphthacene derivatives: synthesis and biological properties.


Traumatic lesions of the ankle joint. The supination-external rotation mechanism.


Camazepam versus diazepam: a double-blind study during endoscopy inspection.


Visual suppression of vestibular nystagmus in rhesus monkeys.


Study of a new antihypertensive (guanabenz).


Familial IgA defects.

Familial IgA defects.


Microbial models of mammalian metabolism. Aromatic hydroxylation.


Lymphocyte-macrophage interaction in resistance to Listeria momocytogenes.


Scrub typhus antibody in mammals in three habitats in Sabah.


Changes in conformation and nucleotide binding of Ca, Mn, or MgG-actin upon removal of the bound divalent cation. Studies of ultraviolet difference spectra and optical rotation.


Accumulation of 3H-1-lysine by brain and plasma in male and female rats treated acutely wigh morphine sulfate.


Protein calorie malnutrition: a major health problem of multiple causation in Malaysia.


The molecular biology of Euglena gracilis. IX. Amino acid pool composition.


The estimation of serum cholinesterase in the presence of anti-cholinesterase insecticides.


Antibodies against different viral antigens in cerebrospinal fluid of patients with multiple sclerosis and other neurological diseases.


Physiologic and pathophysiologic relationship between the electroencephalogram and the regional cerebral blood flow.


Multiple myeloma. I. Clinical, biochemical and immunochemical aspects.


The ultrastructure of melanoma cells treated with 5-bromodeoxyuridine.


Cannabis: a psychopharmacological review.


Browning of the lens in generalized albinism.


Circulating autoantibodies in ocular pemphigoid.


The production of antibodies against mammalian LDH-1.


Hydroxyapatite column chromatography in procedures for isolation of purified DNA.


Testicular lesions in rats treated for one year with ethambutol in low doses.


Raised intracranial pressure and cerebral blood flow. 3. Venous outflow tract pressures and vascular resistances in experimental intracranial hypertension. Pressure changes within the venous outflow tract from the brain were studied in anaesthetized baboons. Segmental vascular resistance changes were also calculated and the results correlated with the changes in cerebral blood flow, measured by the (133)Xenon clearance method. Three different methods were used to raise intracranial pressure: cisterna magna infusion, a supratentorial subdural balloon, and an infratentorial subdural balloon. A close correlation was found between the cortical vein pressure and intracranial pressure with all methods of raising intracranial pressure: the overall correlation coefficient was 0·98. In the majority of animals sagittal sinus pressure showed little change through a wide range of intracranial pressure. In three of the six animals in the cisterna magna infusion group, however, sagittal sinus pressure increased to levels approaching the intracranial pressure during the later stages of intracranial hypertension. Jugular venous pressure showed little change with increasing intracranial pressure. The relationship between cerebral prefusion pressure and cerebral blood flow differed according to the method of increasing intracranial pressure. This was due to differing patterns of change in prevenous vascular resistance as venous resistance increased progressively with increasing pressure in all three groups. The present results confirm, therefore, the validity of the current definition of cerebral perfusion pressure-that is, cerebral perfusion pressure is equal to mean arterial pressure minus mean intracranial pressure-by demonstrating that intracranial pressure does represent the effective cerebral venous outflow pressure.


Hepatic blood flow and portal vein pressure during intravenous infusion of amino acids in dogs.


Stereotactic amygdaloidotomy for aggressive behaviour. Amygdaloidotomy was performed bilaterally on 15 and unilaterally on three patients exhibiting severe aggressive or self-mutilating behaviour. Nine subjects (50%) were improved a year after operation; improvement was maintained in seven (39%) for periods ranging from 27 months to nearly six years. Four non-epileptic cases had convulsions during the period of review; one of them has a persistent mild hemiparesis dating from the postoperative period. There was a tendency for epileptics to respond better than non-epileptics and for mentally retarded patients to respond poorly, but none of the differences was statistically significant.


Isocitrate lyase from the free-living nematode, Tubatrix aceti: purification and properties.


Intestinal alkaline phosphatase. Physical properties and quaternary structure.


Effects of abnormal base ionizations on Mg2 plus binding to transfer ribonucleic acid as studied by a fluorescent probe.


Energetics and spectral changes in ligand binding by homogeneous rabbit anti-lactose antibody.


Proceedings: Evaluation of a rat thyrotrophin radioimmunoassay and its applications.


Immune response to Mycobacterium leprae in indeterminate leprosy patients.


Hydrolysis of casein by three extracellular proteolytic enzymes from Staphylococcus aureus, strain V8.


Agglutinins against group 3 atypical mycobacteria.


Current understanding of human prolactin physiology and its diagnostic and therapeutic applications: a review.


The formation of a calcium-dependent extracellular proteolytic enzyme from Staphylococcus aureus.


Rabbit anti rat lymphocyte serum: immunosuppression mediated by a pure IgM fraction.


An orthopantomographic study of developmental mandibular bone defects (Stafne's idiopathic bone cavities).


Relation between fragments after oblique sliding osteotomy of the mandibular rami and its influence on postoperative conditions.


The relationship between hyphal branching, specific growth rate and colony radial growth rate in Penicillium chrysogenum.


Cellular and humoral pathways in the neuroendocrine regulation of reproductive function. Studies carried out in recent years, including those conducted by the author on the rhesus monkey, which have added more information on the possible cellular and humoral pathways by which the neural and endocrine functions concerned with  reproductive phenomena are integrated are reviewed.  The possible involvement of the circumventricular structures, especially the tanycyte ependyma and the pineal gland in the detection of the endogenous levels of gonadal hormones, and the cerebrospinal fluid (CSF) in the transmission of  hormonal information to the various parts of the brain indicated further areas of research to be explored.  Such exploration should be conducted in diverse, yet complimentary, disciplines so that the whole spectrum of events known to occur in the neuroendocrine regulation of reproduction can be studied.


Editorial: Coming of age of medical primatology.


The role of calcium for stability and activity of an extracellular proteolytic enzyme from Staphylococcus aureus.


Comparative study of radiosensitivity of peripheral blood lymphocyte chromosomes of Macaca mulatta at different doses of irradiation.


Blood groups of mountain gorillas (Gorilla gorilla beringei).


Ultrastructural observations on scrapie in the gerbil.


Inoculation of cattle with bovine lymphoid cell lines infected with Theileria annulata.


Studies on menstrual blood loss in intact and experimental rhesus monkeys.


African swine fever: comparison of four serotests on porcine serums in Spain.


Woodruffia metabolica: an exception to the rule of desmodexy. Woodruffia metabolica (Protozoa, Ciliophora) has a compound fiber on the left side of its ciliary rows composed of parallel, overlapping ribbons of kinetosome-based microtubules. No similar structure is found connecting the kinetosomes on the right side of the ciliary rows. This arrangement is in opposition to the rule of desmodexy.


To the possible spread of scabies through bed linen.


Pair formation in tetrahymena pyriformis, an inducible developmental system.


Glycogen synthetase and phosphorylase in developing chick glycogen body.


Community health education: a challenge to hospital and medical staffs.


The composition of several commercial rapid tube and saline anti-D reagents.


Immunologic reactivity in rabbits remotely deprived of the sacculus rotundus.


Conductance of active and passive pathways in the toad bladder.


Aldosterone stimulation of acidification of urine by isolated urinary bladder of the Colombian toad.


Evolution of a facilitated diffusion pathway for amides in the erythrocyte.


Control of cell division in bacteria.

Control of cell division in bacteria.


Stimulation by amphotericin B of active Na transport across amphibian cornea.


Spatial distribution of human cell survival and oxygen effect in a therapeutic helium ion beam.


ISAH: a versatile treatment positioner for external radiation therapy.


Radiation therapy of Hodgkin's disease: significance of splenic involvement.


Spatial distribution of human cell survival in a neutron bean designed for therapy.


Theoretical explanations of drug-dissociated behaviors.


Hallucinogens as discriminative stimuli.


Experience with fast neutron therapy usine the Texas A and M variable energy cyclotron.


Experimental inhibition of serotonin by antibiotic: prevention of cerebral vasospasm.


Continuous nasogastric infusion feedings of infants of low birth weight.


Feeding the low birth weight infant: orally or parenterally? Preliminary results of a comparative study.


Prenatal diagnosis and selective abortion for anencephaly and spina bifida.


Simple method for detecting amylase inhibitors in biological materials.


Interferon response of the fetal Rhesus monkey after viral infection.


Proceedings: 320. Visuokinetic neurons of the prefrontal cortex and delayed response.


Proceedings: 314. Group I activated neurons of 3a area and voluntary forearm movement in the monkey.


Proceedings: 362. Response of the amphibian papilla nerve in the toad bufomarinus.


Surface pattern of some microsporidian spores as seen in the scanning electron microscope.


Effects of age on the susceptibility of larvae and nymphs of Ixodes ricinus (L.) to insecticides.


The protozoan flora of potable water from natural sources and its sub-clinical implications.


Nitrogen metabolism and ultrastructure in Anabaena cylindrica. I. The effect of nitrogen starvation.


Bladder diverticula in infants and children.


High pressure voiding urethrography. Principle and technique.


Photochemical disproportionation of sulfur into sulfide and sulfate by Chlorobium limicola forma thiosulfatophilum.


Prolactin and mental illness.

Prolactin and mental illness.


A Welsh forensic psychiatric service for doctors.


Oculofacial anomalies and corneal ulceration.


rDNA magnification at the bobbed locus of the Y chromosome in Drosophila melanogaster.


Management of depressed fractures of the skull.


Protein-calorie malnutrition in Fiji (a clinical survey of malnourished children, admitted to the Colonial War Memorial Hospital, Suva).


Aeruginocin tolerant mutants of Pseudomonas aeruginosa.


Beta2-microglobulin: occurrence in fetal life and malignancy.


Evidence for membrane cholesterol as the common binding site for cereolysin, streptolysin O and saponin.


Metallic copper as an intrauterine contraceptive agent.


Vasopressin release from the isolated neurohypophysis induced by a calcium ionophore, X-537A.


An evaluation of 1-(4-methyl) 2, 5-dimethoxyphenyl-2-aminobutane (MDPA-1) as an enhancer of memory and learning.


Prolonged parenteral nutrition in the home.


Fatty acid-induced tuberculocidal activity in sera of guinea pigs treated with bacillus Calmette-Guérin and lipopolysaccharide.


The small intestinal lesion induced by Hawaii agent acute infectious nonbacterial gastroenteritis.


Chemistry and enzymology of kcat inhibitors.


Fetal malnutrition.

Fetal malnutrition. Fetal malnutrition has been associated with maturational defect in cell metabolism.  A series of studies on anthropometric and clinical aspects of patients with intrauterine malnutrition was conducted to establish the relationship between intrauterine growth failure and cell function.  The 1st study assessed intrauterine growth in 128 cases.  36 infants were found to be small for gestational age and exhibited usual signs of fetal growth retardation.  The mothers of these infants were similar with respect to age, weight, height, nutritional patterns, and prior pregnancy histories.  The 2nd study determined whether there was a unique pattern for certain cell energy function in leukocytes of mothers and their small-for-gestational age infants at the time of birth, and whether this pattern was the same as that found in infants with severe postnatal protein-calorie malnutrition.  The findings showed that the altered pattern of energy metabolism in leukocytes of infants with intrauterine growth retardation was the same as that found in young infants with postnatal protein-calorie malnutrition.  The similarity of enzyme pattern in the adenine nucleotide contents of maternal and cord blood leukocytes at time of delivery also supported the interrelationship between energy metabolisms of mothers and their fetuses.  The 3rd study suggested that fetal malnutrition may be reflected in placental tissues.  These studies may prove useful in developing diagnostic tools for monitoring fetal growth and for establishing criteria for intervention.


A thin, flat, parallel plate ionization chamber for electron beam depth-dose measurements.


Methylmalonic acid excretion studies.

Methylmalonic acid excretion studies.


Histones of Drosophila embryos. Electrophoretic isolation and structural studies.


Activation of succinate dehydrogenase by FMNH2 and by photoreduction.


Trimethoprim-sensitivity testing and thymineless mutants.


Site of attachment of J chain to human immunoglobulin M.


Role of myosin light chains in calcium regulation.


Partial characterisation of Escherichia coli haemolysin.


Diagnosis of subacute sclerosing panencephalitis by serial tissue co-cultivation.


Thyrotropin-releasing hormone: regional distribution in rat brain. A sensitive and specific radioimmnunoassay has been used to measure the distribution of thyrotropin-releasing hormone (TRH) in rat brain. All areas of brain tested, except cerebellum, contained readily measurable amounts of TRH. The hypothalamus contained only 31.2 percent of the total brain content of TRH. These results support recent suggestions of central actions for TRH in addition to its hypophysiotropic functions.


Thyrotropin-releasing hormone in specific nuclei of rat brain. The regional distribution of thyrotropin-releasing hormone (TRH) in rat brain was studied. The greatest concentration of TRH was found in the median eminence. High concentrations were also found in several hypothalamic nuclei. Outside the hypothalamus, relatively large amounts of TRH were found in the septal and preoptic areas.


Symptomatic arachnoid diverticula.

Symptomatic arachnoid diverticula.


Periventricular leukoencephalopathy in the monkey. A search for the "normal control" and the "early lesion".


The nature of Encephalitozoon brumpti Coulon, 1924.


Hypothalamic releasing factors and Parkinson disease.


Effects of L-tryptophan, L-3, 4, dihydroxyphenylalanine and tranylcypromine on the electroencephalogram and on photically induced epilepsy in the baboon, Papio papio.


Rebound eating following carbachol-induced drinking in rats.


Equine infectious anemia.

Equine infectious anemia.


A contact detection system for identifying individual animals in groups.


Activities of DNA degrading enzymes in the slime mold Physarum polycephalum. Evidence for five different enzymes.


The effect of fixation on the wave propagation of the protozoan axostyle.


Utile and futile cycles in the liver.

Utile and futile cycles in the liver.


Are segments of chloroplast DNA differentially amplified?


Enhanced specificity of epinephrine binding by rat liver plasma membranes in the presence of EDTA.


Calcium ion regulation of flagellar beat symmetry in reactivated sea urchin spermatozoa.


Ultrastructural localization of cell wall teichoic acids in Streptococcus faecalis by means of concanavalin A.


The basic nuclear proteins of the cellular slime mold Dictyostelium discoideum.


Lung contamination by Ce and La. Evolution of the early spontaneous absorption as a function of the initial lung burden.


The influence of EDTA on the composition of alginate synthesized by Azotobacter vinelandii.


Retention of inhaled antimony-124 in the beagle dog as a function of temperature of aerosol formation.


Investigations on radiosensitive and radioresistant populations of Drosophila melanogaster. IV. The genetics of the relative radioresistance in stage-7 oocytes of the irradiated population RO I.


The induction of aneuploidy by colcemid fed to Drosophila melanogaster females.


Heterologous interchange and non-disjunction of distributively paired chromosomes in Drosophila melanogaster: immature oocytes.


Genetically significant dose from beam therapy in Japan, 1971.


Growth of surface colonies of the gliding bacterium Myxococcus xanthus.


Participation of two photosystems in the photo-phobotaxis of Phormidium uncinatum.


The reproductive cycle of the gelada baboon.


Ozone effects on chlorophylls a and b.

Ozone effects on chlorophylls a and b.


Indentification of a pH 6.5 acetohydroxyacid synthetase in Bacillus subtilis.


Tobacco budworm: chemosterilization by fumigation with thiotepa.


Corynebacterium diphtheriae infections in Tanzania.


Immunisation of marmoset monkeys with a killed oncogenic herpesvirus.


Effect of anti-C4 on cellular synthesis of C4 by guinea pig peritoneal macrophages, pulmonary macrophages, liver or spleen cells.


Intraluminal duodenal diverticulum.

Intraluminal duodenal diverticulum.


Preparation of penicillin N and isopenicillin N.


Isolation and properties of recombination-deficient mutants of Pseudomonas aeruginosa.


Localization of chemically induced breaks in chromosomes of human leucocytes.


Recent studies on the origins of cellular organelles.


Studies of ribosomal diffusion coefficients using laser light-scattering spectroscopy. Using an optical beating technique, the diffusion coefficients and relative scattered intensity of Escherichia coli 70S, 50S, and 30S ribosomes are measured as a function of temperature and Mg(2+) concentration. For solutions at 10 mM Mg(2+) and between 0 degrees C and about 40 degrees C, the values of D(20,w) obtained are 1.7, 1.9, and approximately 2.1 x 10(-7) cm(2)/s, respectively. Preparative procedures drastically affect these values and equivalent hydrodynamic ellipsoids of revolution models give large axial ratios indicating extensive hydration or a deviation from the assumed shape. Calculations also indicate that the subunits expand upon dissociation. Measurements of D(20,w) vs. temperature indicate that 70S particles undergo a conformational change prior to dissociation and can be heat dissociated at 30-32 degrees C at low concentrations. Treatment of 70S ribosomes with EDTA causes a biphasic dissociation reaction. Addition of Mg(2+) after dissociation with EDTA shows that longer waiting times yield fewer 70S particles and that even short waiting times may yield ribosomes differing from the native conformation. Addition of p-chloromercuribenzoic acid (PCMB) is shown to dissociate 70S particles, but to a lesser extent than ethylenediaminetetraacetic acid (EDTA).


Glucose-6-phosphate dehydrogenase Jackson. A new variant associated with hemolytic anemia.


Serologic recognition of the histocompatibility antigens using agglutination and cytotoxicity techniques.


Complement-mediated changes in morphology of hepatitis B antigen-antibody complexes.


Cell wall alterations of gram-negative bacteria by aminoglycoside antibiotics. After treatment with aminoglycoside antibiotics, Escherichia coli B and Pseudomonas aeruginosa P28 showed numerous blebs on the cell wall surface.


Differences between Bombay and Rhnull phenotypes.


Time-related changes in reaction determining hepatitis B antigen in viral hepatitis patients.


Differentiation of Drosophila neuroblasts to form ganglion-like clusters of neurons in vitro.


The effect of humoral agents on the cranial circulation of the monkey.


In vitro immunological characteristics of lymphoid cells  derived from owl monkeys infected with Herpesvirus saimiri.


Immunological and virological investigations on owl monkeys infected with Herpesvirus saimiri.


Chemotherapy of Herpesvirus saimiri induced lymphoma-leukemia in the owl monkey.


Specificity differentiation (SPD) of oncogenic and non-oncogenic herpesviruses by immunofluorescence.


Immunochemical quantitation of dextran by a polymer enhanced nephelometric procedure.


Cortisone-resistant thymocytes as target cells in lymphocyte-mediated cytolysis.


Iron absorption from maize. Effect of ascorbic acid on iron absorption from maize supplemented with ferrous sulphate.


The effects of orbital and dorsolateral frontal cortical ablations on ethanol self-selection and emotional behaviors in monkeys (Macaca nemestrina).


Detection of brief light flashes by monkeys (Macaca nemestrina) with dorsolateral frontal ablations.


Membrane-bound DD-carboxypeptidase and LD-transpeptidase of Streptococcus faecalis ATCC 9790.


Composition of lipopolysaccharides from Myxococcus fulvus and other fruiting and non-fruiting myxobacteria.


Reactive hyperaemia in the dog heart: studies on mechanisms.


Mutagenicity of reactive derivatives of carcinogenic hydrocarbons: evidence of DNA repair.


Some kinetic properties of human-milk galactosyl transferase.


Pathogenesis of anemia associated with Mycoplasma pneumoniae.


Bilateral ossification of the ear canal in an American Cocker Spaniel.


The immunology of experimental Chagas' disease. 3. Rejection of allogeneic heart cells in vitro. Experiments that consisted of incubation of Trypanosoma cruzi-sensitized lymphocytes derived from chronically infected rabbits and from rabbits repeatedly immunized with a small particle or membrane fraction derived from homogenates of T. cruzi forms, showed destruction of allogeneic, parasitized and nonparasitized heart cells in vitro. Mononuclear cells collected from peripheral blood were incubated for 1 h at 37 degrees C to isolate the lymphocytes. Following incubation, over 99% of the cells in the supernate were lymphocytes, which were utilized in these experiments. At the start of these experiments, 70-80% of the sensitized lymphocytes were unattached, small and round, with sparse filipodia. In the ensuing hours, marked heart cell destruction, similar to that seen in an active lesion when lymphocytes invade heart tissue, were observed. After 18 h incubation, about 65-70% of the lymphocytes were attached, larger, and rough surfaced. Inhibition of monocyte migration tests, each in the presence of the antigens of subcellular fractions of T. cruzi organisms and of allogeneic heart myofibers, indicated the presence of a cross-reacting antigen common to both the parasite and the heart in the small particle or membrane fractions. The particulate antigens of the 30,000 g, 35-min fraction of heart muscle gave rise to inhibition of monocyte migration as did the counterpart fraction derived from T. cruzi organisms. The destruction of nonparasitized target heart cells by T. cruzi-sensitized lymphocytes is an in vitro model of the chronic myocarditis of Chagas' disease, and the recognition of cross-reactive antigens of the host cell by T. cruzi-sensitized lymphocytes is believed to be the pathogenic basis for subsequent tissue injury in the chronic phase of this disease.


Role of meningococcal endotoxin in meningococcal purpura. Enteric bacilli and meningococci (MGC) both contain potent endotoxins, but purpuric skin lesions indistinguishable from the experimental dermal Shwartzman reaction are much more common during meningococcal bacteremia than during bacteremia with enteric organisms. Highly purified lipopolysaccharides (LPS), virtually free of contamination by protein, RNA, and capsule, were extracted by a modification of the phenol-water technique from MGC (serogroups A, B, and C) and enteric bacilli (Escherichia coli 04 and 0:111, and Salmonella typhimurium). Polysaccharides in these LPS were similar by gas chromatography except for one galactose-deficient strain of MGC (135B). LPS from MGC and enterics were equally potent for the general Shwartzman reaction and mouse lethality, but LPS from MGC was 5-10 times more potent in inducing the dermal Shwartzman reaction. The greater skin potency of LPS from MGC explains the prominence of purpura in meningococcemia. Comparison of the properties of LPS may explain other differences in clinical syndromes caused by gram-negative bacteria.


Restoration of mycolate synthetase activity in Mycobacterium tuberculosis exposed to isoniazid.


Niacin-negative Mycobacterium tuberculosis.


Primary drug resistance: a continuing study of drug resistance in tuberculosis in a veteran population within the United States. X. September 1970 to September 1973.


Primary drug resistance in children. Drug susceptibility of strains of M. tuberculosis isolated from children during the years 1969 to 1972 at the Kings County Hospital Medical Center of Brooklyn.


Letter: Protein calorie malnutrition, gastric motility and diabetes.


Evidence for cellular control in the synthesis of acetoin or alpha-ketoisovaleric acid by microorganisms.


The influence of culture conditions on the antigenic products of Saprolegnia.