Physicochemical characteristics of a bovine enterovirus prototype.


Syndactyly in an Aberdeen Angus calf.

Syndactyly in an Aberdeen Angus calf.


Characterization of Cal-1 strain of acute Marek's disease agent.


Ribonucleases.

Ribonucleases.


Enzymes in DNA metabolism.

Enzymes in DNA metabolism.


Polypeptide hormones.

Polypeptide hormones.


Management of nonpalpable breast carcinoma discovered by mammography.


Chemical synthesis of peptides.

Chemical synthesis of peptides.


Nucleic acid metabolism in the ruminant. Determination of nucleic acids in digesta.


Paralysing venoms of solitary wasps.

Paralysing venoms of solitary wasps.


Temporal relationships of puromycin and ACTH effects on cultured hamster adrenal glands.


The presence of alkenyl and alkyl glycerides, and choline and ethanolamine plasmalogens in a marine worm.


Lipids of the larvae and adults of Trogoderma granarium (Coleoptera).


Effect of hydroxylamine on respiration and oxidative phosphorylation.


The effect of tegretol on some elementary neuronal mechanisms.


The subcellular distribution of acyltransferases which catalyze the synthesis of phosphoglycerides.


Studies on the serum proteins of chimeras. 3. Detection of donor-type C'5 in allogeneic and congenic post-irradiation chimeras.


Electron microscopic studies of anterior pituitary glands of vitamin E-deficient male mice.


Correlation of electron microscopic and biochemical studies on constricted monoaminergic nerves.


The effects of theophylline, diazoxide and imidazole on isoproterenol-induced inhibition of the rabbit ileum.


The effect of digitalis on the excitability of autonomic nerves.


Inhibitory effects of alloxan on mammalian adenyl cyclase.


An ultrastructural study of the adrenocorticotrophic hormone-secreting cell in the rat adenohypophysis during adrenal cortical regeneration.


A comparison of the properties of pyruvate kinase from hepatoma 3924-A, normal liver and muscle.


Reduced adenosine 3',5'-monophosphate phosphodiesterase activity in the pancreas and adipose tissue of spontaneously diabetic mice.


Mg2+-induced shrinkage in mitochondria isolated from Yoshida ascites hepatoma.


Formation of prototrophs in mixtures of two auxotrophic mutants of Serratia marcescens HY by a transducing bacteriophage produced by some auxotrophs.


Cyclic adenosine monophosphate: possible mediator for norepinephrine effects on cerebellar Purkinje cells. Microelectrophoretic application of norepinephrine or cyclic adenosine monophosphate reduces the discharge frequency of Purkinje cells in the rat cerebellum. In contrast, other nucleotides accelerate the discharge rate of most units. Parenterally administered theophylline, which inhibits the hydrolysis of cyclic adenosine monophosphate enhances the effects of norepinephrine and cyclic adenosine monophosphate. Therefore, norepinephrine may be able to regulate Purkinje cells functionally by metabolic stimulation of cyclic adenosine monophosphate synthesis.


C-fiber responses in the ventrolateral column of the cat spinal cord. Saphenous nerve C-fiber volleys generate, in the ventrolateral column of the cat spinal cord, a conducted postsynaptic response revealed by averaging the integrated temporally dispersed axonal discharges. This finding is compatible with the hypothesis that the feline ventrolateral column includes a major pathway related to nociception.


The minimal infectious dose of adenovirus type 4; the case for natural transmission by viral aerosol.


Diseases of the facial nerve.

Diseases of the facial nerve.


A clinical syndrome and EEG-sleep changes associated with amino acid deprivation.


The stability of angiotensinamide in aqueous solution.


Biochemical studies on marine shellfish lipid. II. Glycolipid of oyster mantle.


Studies on cytochrome b-555 from larvae of the housefly, Musca domestica L. I. Purification and properties of cytochrome b-555.


Nucleotide sequence of tyrosine transfer RNA from Torulopsis utilis.


Recovery of cholinesterase activity in organophosphate treated insects.


Phosphatases and differentiation of the Golgi apparatus.


Electron microscopic studies of polyoma DNA released in protein monolayers.


Allosteric changes in citrate synthase observed by electron microscopy.


Interaction between cytomegalovirus and Newcastle disease virus as mediated by intrinsic interference. Cytomegalovirus (CMV) was demonstrated to induce intrinsic interference to Newcastle disease virus (NDV) in human fibroblast cells under noncytopathic conditions. This interference is unique in that (i) cytomegalovirus is the first DNA virus demonstrated to have this property and (ii) the state of interference was transient and progressively lost as the condition of the cells changed with the development of cytopathic effect. These observations are consistent with the view that the newly formed protein responsible for interference with NDV has a limited half-life and is no longer made when cytopathic conditions are produced by CMV.


Influence of the rate of cell growth and cell density on interferon action in chick embryo cells. Chick embryo cells became more sensitive to the action of interferon the longer they remained in culture. This phenomenon was found even before confluency had been reached. The relative insensitivity of newly seeded cells was not due to a loss of receptors. Cells synthesizing deoxyribonucleic acid (DNA) at a high rate were less sensitive to interferon action than cells synthesizing DNA at a low rate, but the inhibition of DNA synthesis had no effect on interferon action. An increase in the number of cells used for seeding resulted in an earlier appearance of increased sensitivity to interferon action. These results are discussed in relation to the induction process in animal cells.


Ribonucleic acid synthesis in cells infected with herpes simplex virus. I. Patterns of ribonucleic acid synthesis in productively infected cells. HEp-2 cells were pulse-labeled at different times after infection with herpes simplex virus, and nuclear ribonucleic acid (RNA) and cytoplasmic RNA were examined. The data showed the following: (i) Analysis by acrylamide gel electrophoresis of cytoplasmic RNA of cells infected at high multiplicities [80 to 200 plaque-forming units (PFU)/cell] revealed that ribosomal RNA (rRNA) synthesis falls to less than 10% of control (uninfected cell) values by 5 hr after infection. The synthesis of 4S RNA also declined but not as rapidly, and at its lowest level it was still 20% of control values. At lower multiplicities (20 PFU), the rate of inhibition was slower than at high multiplicities. However, at all multiplicities the rates of inhibition of 18S and 28S rRNA remained identical and higher than that of 4S RNA. (ii) Analysis of nuclear RNA of cells infected at high multiplicities by sucrose density gradient centrifugation showed that the synthesis and methylation of 45S rRNA precursor continued at a reduced but significant rate (ca. 30% of control values) at times after infection when no radioactive uridine was incorporated or could be chased into 28S and 18S rRNA. This indicates that the inhibition of rRNA synthesis after herpesvirus infection is a result of two processes: a decrease in the rate of synthesis of 45S RNA and a decrease in the rate of processing of that 45S RNA that is synthesized. (iii) Hybridization of nuclear and cytoplasmic RNA of infected cells with herpesvirus DNA revealed that a significant proportion of the total viral RNA in the nucleus has a sedimentation coefficient of 50S or greater. The sedimentation coefficient of virus-specific RNA associated with cytoplasmic polyribosomes is smaller with a maximum at 16S to 20S, but there is some rapidly sedimenting RNA (> 28S) here too. (iv) Finally, there was leakage of low-molecular weight (4S) RNA from infected cells, the leakage being approximately three-fold that of uninfected cells by approximately 5 hr after infection.


An alkaline phenylbutazone preparation.

An alkaline phenylbutazone preparation.


Diurnal variations of the plasma cortisol level in diabetes insipidus.


Adenosine 3',5' monophosphate as an inhibitor of ovulation and reproduction.


Coumarin-induced skin necrosis in a sixteen-year-old girl.


Study on the antituberculous activity of tuberactinomycin, a new antimicrobial drug.


Nicotinamide adenine dinucleotide levels and nadase activity in polymorphonuclear leukocytes, alveolar macrophages, and erythrocytes of tuberculous guinea pigs.


A naturally occurring indolylpteridine.

A naturally occurring indolylpteridine.


Binding of adenosine 5'-monophosphate and substrate by rabbit liver fructose 1,6-diphosphatase.


Chemical structure of the insoluble hyphal wall glucan of Phytophthora cinnamomi.


The rates of binding of reduced nicotinamide-adenine dinucleotide analogs to liver alcohol dehydrogenase.


The fructose 1,6-diphosphatase of Dictyostelium discoideum.


Regulation of the activity of L-aspartate beta-decarboxylase by a novel allosteric mechanism.


Different allosteric properties of nucleoside diphosphatase isoenzymes from rat liver.


Phosphoramidates. V. Probable identity of rat liver microsomal glucose 6-phosphatase, phosphoramidase, and phosphoramidate-hexose phosphotransferase.


The regulation of glycolysis in mammalian erythrocytes.


Abnormal height in children.

Abnormal height in children.


Hormones and cholesterol ester metabolism.


Ca2+ uptake and acetyl phosphatase of skeletal muscle microsomes. Inhibition by Na+, K+, Li+, and adenosine triphosphate.


Action of micrococcal nuclease on polymers of deoxyadenylic and deoxythymidylic acids.


Isolation, morphology, and composition of the nuclear membrane from rat liver.


Studies on the kinetic mechanism and allosteric nature of bovine brain hexokinase.


Structural studies on polypeptide hormones. I. Fluorescence.


An experimental approach to the study of the folding of staphylococcal nuclease.


The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase (type D) to oxidase (type O).


Evidence for an internal antigen in foot-and-mouth disease virus.


Malignant myoblastoma. A case report.

Malignant myoblastoma. A case report.


Neurocutaneous melanosis and melanomas in children.


Cytomegalovirus infection of the cervix: an "incidental" finding of possible clinical significance. Report of a case.


Prediction of outcome of pregnancy by vaginal cytology. Evaluation of the "estrogen test".


Herpes vulvitis.

Herpes vulvitis.


Systematic transference interpretations in the sleep laboratory. A new approach to psychoanalytic research.


Free radicals in bone grafts sterilized by ionizing radiation.


Massive gangrene of the colon secondary to carcinoma of the rectum. Case report.


Experimental peritonitis in irradiated rabbits.


Morphologic and pathologic aspects of intercalated disc of the heart.


Exfoliative cytopathologic studies in organ transplantation. I. The cytologic diagnosis of cytomegalic inclusion disease in the urine of renal allograft recipients.


Quantitative aspects of lipolysis and reesterification in human adipose tissue in vitro.


Treatment of cancer of the nasal cavity.


Liver irradiation in children: acute changes with transient leukopenia and thrombocytopenia.


Lymphatic and visceral irradiation from endolymphatic infusion in the rabbit.


The diagnostic problems of isolated, circumscribed breast tumors.


Liver and spleen scintiscanning with indium 113m. A clinical pathologic correlation.


Roentgenologic manifestations of malignant tumors of the nasopharynx.


Serial brain scanning with technetium 99m and scintillation camera.


Stage 4 sleep in schizophrenia.

Stage 4 sleep in schizophrenia.


Effects of selected phenothiazines on REM sleep in schizophrenics.


Viral growth patterns in an iris organ culture system.


Neuromuscular disorder following gastric resection.


A bovine strain of Myxovirus parainfluenza type 3. Experimental transmission and a survey of antibodies in cattle in Australia and New Guinea.


The isolation of Myxovirus parainfluenza type 3 from sheep in Australia.


Inhibition of ethanol metabolism in vivo by administration of pyrazole.


Study on the stability of lysosome membranes. V. Changes in the stability of liver lysosomes of rats kept at different temperatures after treatment with chlorpromazine.


Creutzfeldt-Jakob's disease: a histochemical study.


Recurrent and chronic relapsing Guillain-Barré polyneuritis.


Control by brain stem reticular formation of sensory transmission in Burdach nucleus. Analysis of single units.


Mechanism of the effects of furosemide on renin secretion in anesthetized dogs.


Non-carcinogenicity of hexamethylenetetramine in mice and rats.


Carcinogenicity of ponceau MX in the mouse.


A case of metastatic carcinoma of choroid from breast.


My field of research--cell malignancy in vitro.


A sibship with Bartter's syndrome: failure of total adrenalectomy to correct the potassium wasting.


Aplasias of the anterior thoracic wall, the pectoral muscles, and the breast.


Pastes on normal and "white-spot" enamel. The effect of fluoride-containing prophylaxis.


Labortary studies concerning the development of a stannous fluoride-phosphate prophylactic paste.


Human liver alcohol dehydrogenase: inhibition by pyrazole and pyrazole analogs.


Malignant hepatoma presenting as a spinal lesion.


Studies on the stereospecificity of liver alcohol dehydrogenase (LADH) for 3beta-hydroxy-5beta-steroids. Inhibition effect of pyrazole and of a 3alpha-hydroxycholanoic acid.


Studies on plasma angiotensinase activity in normal and toxemic pregnancy.


Histochemical observation on the phosphatase activities of the differentiating taste bud.


Serum ornithine carbamoyl transferase activity (S-OCT) after irradiation of malignant tumours and after administration of ACTH.


Regulation of glycolysis and L-glycerol 3-phosphate concentration in rat epididymal adipose tissue in vitro. Role of phosphofructokinase. 1. Attempts were made to define the role of phosphofructokinase in glycolytic control and the factors regulating the concentration of l-glycerol 3-phosphate in rat epididymal fat pads incubated in vitro. 2. Glycolysis rates were altered by anoxia or by additions of insulin, adrenaline or both to the incubation medium, and the changes in rate were related to changes in the steady-state concentrations of hexose phosphates, adenine nucleotides, l-glycerol 3-phosphate and citrate in the whole tissue. Measurements were also made of the lactate/pyruvate concentration ratio in the medium after incubation. 3. The mass-action ratios of phosphofructokinase, calculated from the whole-tissue concentrations of products and substrates, were less than 0.1% of the value of the ratio at pH7.4 at equilibrium. 4. Only in the presence of adrenaline could the observed stimulation of glycolytic flux be related to a possible activation of phosphofructokinase since, in this situation, the concentration of one substrate, fructose 6-phosphate, was not altered and the concentration of the other, ATP, was decreased. Increased glycolytic flux in the presence of insulin may be explained by an observed increase in the concentration of the substrate, fructose 6-phosphate. Under anaerobic conditions, glycolytic flux was decreased but this did not appear to be the result of inhibition of phosphofructokinase, since the concentrations of both substrates, fructose 6-phosphate and ATP, were decreased. The changes in glycolytic flux with insulin and anoxia may be secondary to changes in the rate of glucose uptake. 5. Changes in l-glycerol 3-phosphate concentration appear to be related both to changes in the concentration of dihydroxyacetone phosphate and to changes in the NADH/NAD(+) concentration ratio in the cytoplasm. They do not seem to be related directly to alterations in glycolytic rate.


Metabolism of camphors and related compounds. 1. The metabolism of (+/-)-norcamphor, (+)-camphor, (-)-camphor, (+)-epicamphor, (+/-)-camphorquinone, (+/-)-camphane-2,5-dione and camphane was investigated in rabbits. All the compounds except camphane-2,5-dione increased the content of glucuronide in the urine. 2. (+/-)-Norcamphor was reduced to endo-norborneol; (+)-camphor, contrary to expectation, was reduced to (+)-borneol, as well as being hydroxylated to (+)-5-endo-hydroxycamphor and (+)-3-endo-hydroxycamphor, 5-endo-hydroxycamphor being the predominant product. (+)-Epicamphor was reduced mainly to (+)-epiborneol; (+/-)-camphorquinone gave 3-endo-hydroxycamphor and 2-endo-hydroxyepicamphor, the former being the major metabolite. (+/-)-Camphane-2,5-dione was reduced to 5-endo-hydroxycamphor. Camphane was hydroxylated to borneol and epiborneol, the latter predominating. 3. An explanation of these findings is given in terms of steric hindrance and thermodynamic stability. 4. The possibility was investigated that NADH was involved in the reductions.


Persistent respiratory virus infection in tracheal organ cultures.


Renal and adrenal factors in hypertension: diagnostic approaches.


Some enzymic activities and chemical properties of the mesosomes and cytoplasmic membranes of Bacillus licheniformis 6346. 1. The liberation of the vesicles of the mesosomes from protoplasts of Bacillus licheniformis strain 6346 is controlled by the Mg(2+) concentration present during the removal of the walls by lysozyme. 2. The functioning of the cytoplasmic membranes is also critically controlled by the Mg(2+) concentration. 3. The isolated mesosomes and the cytoplasmic membranes differ in their enzymic activities. Both succinate dehydrogenase and NADH oxidase activities are very low or absent from the mesosomes. 4. The cytoplasmic membranes can also be separated into materials of different density. 5. Distribution of the membranes between these fractions is controlled by the Mg(2+) concentration and the growth conditions of the microorganisms.


Physiologic control of arterial pressure.


Composition of rat liver triacylglycerols and diacylglycerols.


Biosynthesis of 6 beta- and 6 alpha-hydroxyprogesterone in the human foetal liver.


Enzyme histochemistry and hormones of the developing gastrointestinal tract of the chick embryo. I. A histochemical and quantitative study of glycogen, uridine diphosphate glucose-glycogen transglucosylase, glucose-6-phosphatase and phosphorylase.


Further cytochemical studies of the mucopolysaccharides of the test cells of Ciona intestinalis L.


Studies on the occurrence and properties of lysosomal phospholipases A1 and A2 and the degradation of phosphatidic acid in rat liver lysosomes.


Affinity labeling of the active site of staphylococcal nuclease. Reactions with bromoacetylated substrate analogues.


Purification and partial characterization of the aminoacyl transfer ribonucleic acid binding enzyme from rabbit reticulocytes.


Porcine heart lactate dehydrogenase. Optical rotatory dispersion, thermodynamics, and kinetics of binding reactions.


Lipid peroxidation in vitro by isolated fat cells of rats. Correlation with total lipolysis, glucose utilization, and dietary tocopherol.


Reduction of alpha-ketoglutarate by homogeneous lactic dehydrogenase X of testicular tissue.


Energy-linked ion translocation in submitochondrial particles. I. Ca++ accumulation in submitochondrial particles.


Structural studies on polypeptide hormones. II. Polarization of fluorescence.


Coenzyme A- and nicotinamide adenine dinucleotide-dependent branched chain alpha-keto acid dehydrogenase. I. Purification and properties of the enzyme from Bacillus subtilis.


Cyclic guanosine 3',5'-monophosphate in mammalian tissues and urine.


Amino acid transport by isolated mammalian renal tubules. II. Transport systems for L-proline.


Organization of inhibition in abdominal ganglion of Aplysia. I. Role of inhibition and disinhibition in transforming neural activity.


Organization of inhibition in abdominal ganglion of Aplysia. II. Posttetanic potentiation, heterosynaptic depression, and increments in frequency of inhibitory postsynaptic potentials.


Organization of inhibition in abdominal ganglion of Aplysia. 3. Interneurons mediating inhibition.


Some quantitative aspects of pyramidal collateral inhibition.


Effects of eye movement, brain-stem stimulation, and alertness on transmission through lateral geniculate body of monkey.


The use of 5-iodo-2'-deoxyuridine (IUDR) in Orabase and plastibase for treatment of oral herpes simplex.


Role of angiotensin in normal blood pressure regulation.


The synthesis of deoxyribonucleic acid and nuclear proteins by rat hepatoma cells immediately after gamma irradiation.


ESR-studies of normal and 5-bromouracil-substituted DNA of Bacillus subtilis after irradiation with ultraviolet light.


The reaction of hydrogen atoms with DNA.


Experimental infection of calves with bovine adenovirus types 1 and 2.


Failure of chicken antisera against porcine enterovirus strain V13 to neutralized at low dilutions.


Heat inactivation of foot-and-mouth disease virus in blood products.


3-amino-proline from viomycidine.

3-amino-proline from viomycidine.


Quantitative histochemical analysis of cytomegalic nuclear inclusions.


Ultrastructural study of a nerve biopsy from a case of early infantile chronic neuropathy.


Proliferation of mast cells in peripheral nerves during Wallerian degeneration. A radioautographic study.


Electron microscopic observations in mice infected with Japanese encephalitis.


Psychological, psychophysiological, and biochemical correlates of prolonged sleep deprivation.


Transmissible gastro-enteritis of swine (TGE).


Behavior of sulfhydryl groups of yeast alcohol dehydrogenase for 5,5'-dithiobis(2-nitrobenzoic acid).


Effects of corticosteroids on mouse hepatitis virus infection.


Peptide-secreting tumours with special reference to the pancreas.


Allosteric and kinetic properties of a K-dependent acylphosphatase in rat brain synaptosomes and its possible relation to (Na+K) ATPase.


Apparent low levels of ubiquinone in rat and human neoplastic tissues.


Electron paramagnetic resonance study of bovine liver catalase.


Isolation of a synaptic vesicle fraction from guinea pig brain with the use of DEAE-sephadex column chromatography and some of its properties.


Homogeneity and sequential periodate oxidation of a glycopeptide from Taka-amylase A.


Failure of the cirrhotic liver to inactivate renin: evidence for a splanchnic source of renin-like activity.


An enzymatic method for the measurement of asparagine and a new assay of asparaginase activity.


Fate of Sendai virus ribonucleoprotein in virus-infected cells. The cytoplasmic extracts of Ehrlich ascites tumor cells infected with (32)PO(4) and (3)H-leucine-labeled Sendai virus have been examined during the course of infection with respect to sedimentation behavior and buoyant densities of input virus radioactivity. It was found that (32)P and (3)H radioactivities were coincident, and, at 30 min after infection, the bulk of radioactivity was recovered in the polysome region of a sucrose gradient in the position of Sendai virus ribonucleoprotein (210S). The heterogeneity of radioactivity profiles appeared at 1 hr after infection and increased during 6 hr of incubation. The buoyant densities of input virus components were determined by banding in CsCl gradient. Here again the bulk of coincident (32)P and (3)H radioactivity at 30 min after infection banded at the same density as Sendai virus ribonucleoprotein (1.31 g/cm(3).) This component disappeared at 3 hr after infection, and (32)P and (3)H radioactivities were now found in components banded at densities 1.38, 1.41, 1.45, 1.49, and 1.55 g/cm(3). The results presented are consistent with the idea that virus ribonucleoprotein is retained in the cytoplasm of infected cells during at least 6 hr of incubation, being partly deproteinized in the course of infection. The nature of components which banded at rho = 1.41, 1.45, 1.49, and 1.55 as complexes of partly deproteinized ribonucleoprotein with ribosomes will be described in a separate paper.


Relative sensitivities of viruses to different species of interferon. Some viruses were found to be more sensitive than others to the action of interferons from certain species of animals but less sensitive to interferons from other species. Vaccinia virus was the most sensitive to mouse and hamster interferons of five viruses tested, but the least sensitive of these five viruses to human, rabbit, and bat interferons. The relative sensitivities of the viruses to interferons were found to be characteristic for each of the species tested, with those closely related phylogenetically exhibiting similar patterns of relative interferon-induced virus resistance. The amount of synthetic double-stranded polynucleotide polyinosinic acid-polycytidylic acid required to induce resistance to each of the viruses in each of the cell species correlated with the interferon sensitivities of the viruses.


Ribonucleic acid synthesis of vesicular stomatitis virus. I. Species of ribonucleic acid found in Chinese hamster ovary cells infected with plaque-forming and defective particles. Plaque-forming B particles of vesicular stomatitis virus (VSV) induce the synthesis of virus-specific ribonucleic acid (RNA) in Chinese hamster ovary cells, whereas defective T particles do not. Infection with low input multiplicities of B results in the formation of four species of RNA. During infection with high multiplicities, RNA synthesis begins with mainly these four species of RNA but gradually shifts to a new pattern of RNA synthesis involving five other species of RNA. The change can also be induced by superinfection with T at 2.5 hr after infection with a low multiplicity of B. T added at the same time as B prevents virtually all RNA synthesis. Synthesis of the first group of RNA species correlates with the formation of B particles, whereas synthesis of the second group correlates with the formation of T particles. The various species of RNA formed after infection with VSV particles include single-stranded RNA, a completely double-stranded RNA, and RNA with partially double-stranded regions. These observations begin to establish a molecular basis for understanding the ability of T particles to interfere with the growth of B particles.


Transitory free radicals in irradiated animal tissues.


Histochemical studies on the effect of nerve impulses on exogenous catecholamines taken up into the adrenergic nerves of reserpine-pretreated animals.


An E.P.R. study of peptides after U.V. irradiation.


On the mechanism of the neuromuscular blocking action of cobalt ion.


Duration of application and change of a regimen with secondary drugs.


Intracellular pO2 control on excitability and synaptic activability in aplysia and helix identifiable giant neurons.


Influence of statolon on resistance of mice to influenza. Various interferon inducers are known to elicit protection against lethal or infecting doses of certain viral agents. Because of the relatively high morbidity rate of influenza and its seasonal occurrence, we wished to determine whether statolon-induced interferon might be effective in controlling this disease. Mice were treated intraperitoneally with statolon and challenged with influenza A(2) virus by the intranasal route. Although interferon was present in the serum at the time of virus administration, no change in mortality rate was observed. There was, however, a significant increase in the mean survival time of treated animals. Similar results were obtained when Newcastle disease virus was used as the interferon inducer. To determine the effect of the route of challenge, other mice were treated with statolon or Newcastle disease virus and inoculated with mengovirus by the intranasal or intraperitoneal route. The results demonstrated that the treated mice were protected to similar degree against challenge by either route. It is suggested that the relative ineffectiveness of interferon in protecting mice against influenza is due to an intrinsic characteristic of the virus itself rather than the type of interferon induced or the route of virus challenge.


Response of white mice to cells and culture constituents of Clostridium perfringens. Broth cultures of Clostridium perfringens (ATCC 10543) were fractionated by ammonium sulfate precipitation and Sephadex G-150 chromatography. Components isolated, as well as some enzymes present in the culture, were assayed for toxicity by feeding to white mice. Early work indicated that when a meat-fat-starch slurry, infected with C. perfringens, was fed to mice, the intestinal passage time was reduced. By using large numbers of mice as test animals and analyzing the data statistically, we found that C. perfringens and several fractions from the culture supernatant significantly affected the mice. A toxic material present in the supernatant was not identifiable as phospholipase C. Phospholipase C and physphorylcholine affected the intestinal passage time of the mice only when large amounts were given. The enzyme, neuraminidase, and another unidentified compound present in the supernatant affected the passage time when very small amounts were fed to mice.


Preparation of primary bovine corneal epithelial cell cultures for use in virological investigations. Primary and secondary cell cultures in sufficient quantity for use in virological investigations were prepared and were found suitable for growth of animal viruses.


Some morphologic, biologic and metabolic properties of transplantable Kirkman-Robbins hepatoma in hamsters.


Transmission of labyrinthine volleys through the vestibular nuclei during sleep.


The effect of thiazide on the sympathetic nervous system of hypertensive rats.


The biosynthesis of terpenoid quinones.

The biosynthesis of terpenoid quinones.


Induction of lipase and phytase activities in the aleurine tissue of germinating wheat grains.


The structural organization of haem synthesis in rat liver mitochondria. 1. Anaerobic conditions are normally necessary for incorporation of iron into haems and only ferrous iron is used. After addition of succinate to an incubation mixture containing intact or ultrasonically treated mitochondria, Fe(3+) is used, but only if no inhibitors prevent the transfer of electrons from the mitochondrial respiratory chain to oxygen. 2. A dual-wavelength spectrophotometric assay for ferrochelatase is described that has been used for the continuous assay of incorporation of metal ions into porphyrins. Constants are given for the determination of rates of formation of protohaem and cobalt protoporphyrin, mesohaem, cobalt mesoporphyrin and zinc mesoporphyrin. For cobalt mesoporphyrin formation the K(m) for Co(2+) is 11x10(-6)m and that for mesoporphyrin is 5x10(-6)m. 3. An improved method for the separation of inner and outer membranes of mitochondria is described. Mitochondria swollen in hypo-osmotic media were contracted in hyperosmotic potassium chloride solution containing ATP and the outer membranes detached by mild ultrasonic treatment. Sucrose inhibited the ATP-induced contraction and decreased the yield of outer membranes. 4. Ferrochelatase is associated with cytochrome oxidase, which is used as a marker for inner mitochondrial membranes. 5. By using as substrate porphyrin dissolved in phospholipid micelles, ferrochelatase activity of intact mitochondria was shown to be latent, and to be liberated by ultrasonic treatment. 6. No ferrochelatase was detectable in microsomes or soluble cell components.


Autogenous and virus-induced interferons from lines of lymphoblastoid cells.


Thymidine phosphate pools and DNA synthesis after polyoma infection of mouse embryo cells.


An antiserum to ovarian mucinous cyst fluid with colon cancer specificity.


Separation of DNA polymerase from rat liver and hepatomas.


Increased DNA polymerase activity in a series of rat hepatomas.


A role for nicotinamide adenine dinucleotide glycohydrolase in the control of glyceraldehyde-3-phosphate dehydrogenase activity.


Effect of Rous sarcoma virus infection on recovery of nucleolar RNA, ribonucleoprotein, and DNA synthesis from inhibition by actinomycin D.


Application of the fluorescent antibody method in the diagnosis of M. parainfluenzae. II. Identification of newly isolated m. parainfluenzae strains in monkey kidney tissue cultures.


Identification of the Motol virus. 2. Relationship to pox viruses.


Melatonin and its precursors' effects on 131-I uptake by the thyroid gland under different photic conditions.


Sleep deprivation: changes of monoamines and acetylcholine in rat brain.


Polymyositis presenting as distal muscle weakness. A case report.


Effect of living and non-living BCG on translocation of quartz dust from the lungs to their regional lymph nodes. An experimental study on rats.


Effect of BCG on translocation of quartz dust from the lungs to their regional lymph nodes after different BCG and quartz dose. An experimental study on rats.


Effect of variations in time interval between treatment with BCG and quartz dust on translocation of quartz dust from the lungs to their regional lymph nodes. An experimental study on rats.


Participation of the cell membrane in the formation of "autophagic vacuoles".


Autophagocytosis: origin of membrane and hydrolytic enzymes.


Ultrastructure of synaptic terminals in nucleus infundibularis and nucleus supraopticus of Passer domesticus.


Vascular reactions after experimental nerve section, suture, and transplantation.


Effect of reserpine and other drugs on the monoamine metabolism with special reference to the CNS.


Effect of 5-hydroxytryptamine on experimental silicosis in the rat.


Conjugated estrogens (Premarin) for spontaneous and surgical bleeding.


Viral infections of female genital tract.


Substances with antineoplastic activity. 28. Cytostatic and pharmacologic properties of beta-4-methoxybenzoyl-beta-bromacrylic acid sodium salt.


Jaundice, ascites, and hepatomegaly. Recurrence in Chinese male.


Intermittent peritoneal dialysis. A study of medical complications occurring in 16 patients.


Lymphangiography in the evaluation of malignant neoplasms.


Effect of aminogluthethymide on urinary excretion of corticotropin and adrenal hormones.


The rheumatoid factor and antinuclear antibodies in patients with viral hepatitis.


Quantitative studies on the lipolytic response of human subcutaneous and omental adipose tissue to noradrenaline and theophylline.


Teratoma of the orbit.

Teratoma of the orbit.


Hypertelorism, microtia, and facial clefting. A newly described inherited syndrome.


Congenital cytomegalovirus infection associated with multicystic kidney.


The role of collagenase in the alkali-burned cornea.


The precursor of the evoked spindle activity during low voltage fast sleep.


Changing concepts in Crohn's disease.

Changing concepts in Crohn's disease.


Cytomegalovirus infection among renal allograft recipients.


Bioassay of parathyroid hormone in vitro with a stable preparation of adenyl cyclase from rat kidney.


Vascular compression of the optic nerves relieved by anastomosis of carotid artery to jugular vein.


Vincristine neuropathy: an electrophysiological and histological study.


Treatment of myasthenia gravis with adrenocorticotropic hormone (ACTH): massive short-term and maintenance treatment.


Addison's disease and diffuse cerebral sclerosis.


Penicillin-induced interictal discharges from the cat hippocampus. I. Characteristics and topographical features.


Penicillin-induced interictal discharges from the cat hippocampus. II. Mechanisms underlying origin and restriction.


Gross potential from facial nucleus of cat as an index of neural activity in response to glabella tap.


Neuronal behavior and triggering mechanism in cortical epileptic focus.


Changes in evoked responses from facial nucleus of cat with conditioning and extinction of an eye blink.


Changes in correlation between monosynaptic reflexes produced by conditioning afferent volleys.


Comparison of effects of stimulation of Deiters' nucleus and medial longitudinal fasciculus on neck, forelimb, and hindlimb motoneurons.


Epigastric pain, thrombophlebitis, and neurologic deficit.


Viral hepatitis and the Australia-SH antigen.


Ectopic production of an alkaline phosphatase isoenzyme in patients with cancer.


Surgical treatment of the foot and ankle in rheumatoid arthritis.


Free radicals produced in a rigid solution of typtophan by a biphotonic process.


Photochemistry of tobacco mosaic virus RNA. Effect of HCN.


Electron spin resonance studies of the triplet states of monoanionic thymine.


The stimulatory effect of cyclic adenosine 3'5'-monophosphate on DNA-directed synthesis of beta-galactosidase in a cell-free system. A cell-free system allowing for synthesis of beta-galactosidase enzymatic activity has been developed. This system requires DNA containing the beta-galactosidase gene, a cell-free extract of Escherichia coli bacteria, and the low-molecular-weight components necessary for transcription of the DNA and translation of the resulting messenger RNA. Such a system is useful for studying enzyme synthesis, as well as its regulation. The gene for beta-galactosidase is part of the lac operon whose expression is under the control of the lac repressor. In whole cells the lac repressor inhibits almost all of the gene expression for beta-galactosidase. In the cell-free system, we had previously been able to repress about half the gene expression. Adding cyclic adenosine 3'5'-monophosphate to the cell-free system improved the yield of beta-galactosidase enzymatic activity by 8 to 30 times and the efficiency of repression from 50 to 95 per cent.


Biradical spin labeling for nerve membranes. We have explored the behavior of pair interaction in a suitable biradical when it is bound by van der Waals' forces in a nerve membrane. We have concomitantly examined a set of model solvents to represent this situation. It appears that the biradical suffers a restriction of internal motion with a minimal restraint on its external motion in the nerve medium. The biradical is so situated as not to respond to the passage of the action potential.


Immunochemistry of the capsular polysaccharide of an acinetobacter. The capsular polysaccharide of a strain, BD4, of Acinetobacter calco-aceticus (A.c-a), composed of L-rhamnose and D-glucose, 4:1, precipitates all streptococcal group B antisera tested, as well as streptococcal group G antisera and antipneumococcal (Pn) type XXIII serum. Nonreducing end groups of L-rhamnose, known to be major determinants of the antigens giving rise to these antisera, are thus identified in A.c-a, which also precipitates anti-Pn VI sera. Both the rhamnose and glucose in the capsular polysaccharide (S) of Pn VI are linked 1,3-, as is the rhamnose in Pn S II, which also precipitates anti-Pn VI because of this linkage. A.c-a and S II precipitate the same fraction of anti-Pn VI, identifying a second portion of the rhamnose of A.c-a as 1,3-linked. This is confirmed by the stability of a portion of the rhamnose on oxidation of A.c-a with periodate, and 1,3-linked glucose or a periodate-stable branch point is also indicated in the same way. Two features of the fine structure of A.c-a have thus been established and a third indicated.


Electron microscopic localization of enzymes in Mycobacterium tuberculosis.


Cyclic guanosine monophosphate: effects on short-circuit current and water permeability. In the isolated toad bladder, cyclic guanosine monophosphate induces an increase in short-circuit current similar to that produced by cyclic adenosine monophosphate. In contrast, cyclic guanosine monophosphate has no effect on water permeability in this organ. This finding raises the possibility that different and independent intra-cellular secondary messengers may exist.


Experimental herpes encephalitis: crystalline arrays in endoplasmic reticulum.


Extraparticulate chain interaction between different electron transport particles. Interaction or cross-linking between the respiratory chains of the electron transport particles of bacterial origin occurs with a mixture of active and inactive particles. Interaction between bacterial particles and liver sub-mitochondrial particles also occurs. Irradiation of the bacterial particles at 360 nanometers resulted in the destruction of quinone and consequent loss of ability of reduced nicotinamide adenine, dinucleotides to reduce cytochromes b, c(1), c, and a plus a(3). A mixture of both irradiated and untreated particles in the presence of the reduced dinucleotide resulted in the reduction of cytochromes c and a plus a(3), in an amount equivalent to the total concentration of these cytochromes in both types of particles. In contrast, the amount of cytochrome b reduced was equivalent to half the particle concentration or to that observed with the active particles alone. The rate of reduction of cytochromes c and a plus a(3) with the mixture of particles was similar to that with the active particles alone. The interaction or cross-linking between the particulate respiratory chains of bacteria or of bacterial and mammalian systems occurs after cytochrome b and before or at cytochrome c.


A comparative study of the DNA density and behavior in tissue cultures of fourteen different herpesviruses.


Characteristics of two new avian tumor virus subgroups.


Tumors of salivary glands.

Tumors of salivary glands.


Search for viral etiology of human leukemia and lymphomas: past efforts and future perspectives.


Assay of the antibiotic activity of serum. One of the drawbacks of the "tube dilution" method for the assay of antibiotics in human serum has been illustrated by utilizing serum-sensitive and serum-resistant strains of Escherichia coli. In the case of serum-sensitive strains, it was found that fresh serum alone may account for the same degree of inhibition and thus yield minimal inhibitory concentrations identical to those obtained with serum combined with antibiotics, that is, "simulated" serum assay specimens. This fallacy of the method is discussed with regard to those instances in which laboratories were merely to utilize the patient's own coliform organism as the test organism, or with respect to the assay of, for example, polymyxins, in which inadvertently a R(ough) and therefore, serum-sensitive strain of E. coli were to be used as the indicator organism. It is recommended that serum-resistant laboratory strains of Staphylococcus aureus or E. coli of known antibiotic susceptibility be employed as the test organisms proper in order to circumvent the inherent bactericidal activity of serum.


Quantitative thin-layer chromatographic determination of hippuric acid in rat urine.


The roles of synthesis and degradation in the regulation of catalase levels in the neoplastic tissues.


Rhinoviruses and parainfluenza viruses of horses.


Comments on rhinoviruses and parainfluenza viruses of horses.


Report of the panel for the symposium on immunity to selected equine infectious diseases. Summary.


Identification of adenosine-3',5'-monophosphate as the bacterial attractant for myxamoebae of Dictyostelium discoideum. Adenosine-3',5'-cyclic monophosphate was shown to be the compound found in Escherichia coli responsible for the attraction of the amoebae of the cellular slime mold Dictyostelium discoideum. A number of other nucleotides were tested and the following were active: tubercidin-3',5'-cyclic monophosphate, N(6)-2'-O-dibutyryl-adenosine-3',5'-cyclic monophosphate, 5'-methylene adenosine-3',5'-cyclic monophosphonate, guanosine-3',5'-cyclic monophosphate, uridine-3',5'-cyclic monophosphate, cytidine-3',5'-cyclic monophosphate, inosine-3',5'-cyclic monophosphate, and thymidine-3',5'-cyclic monophosphate. They were less active than adenosine-3',5'-cyclic monophosphate. It is suggested that cyclic adenosine monophosphate secreted by the bacteria is used by the amoebae as a means of sensing and orienting towards food.


Inhibition of protease production of various bacteria by ammonium salts: its effect on toxin production and virulence. Production of protease by many bacteria was found to be inhibited by ammonium salts, and the enzyme production was more sensitive to the salts than was growth of the organisms. Inhibition of protease production by some pathogenic bacteria may result in the recognition of an exotoxin which otherwise would have been digested by the protease. In the case of Pseudomonas aeruginosa, qualitatively different toxicities could be demonstrated in the culture fluids, depending on the presence or absence of protease in such a fluid. The toxicity of the culture in the presence of a high titer of protease may be due primarily to the protease, whereas the toxicity exhibited in the absence of protease could be due to proteinacious exotoxin. Producers of high titers of protease tended to be less virulent in vivo than producers of low titers of the enzyme, which exert their toxicities by a separate exotoxin.


Comparison of lipid effects on K+-Mg2+ activated p-nitrophenyl phosphatase and Na+-K+-Mg2+ activated adenosine triphosphatase of membrane.


Metabolism of the phosphoinositides in guinea-pig brain synaptosomes.


Early events in herpes simplex virus infection: a radioautographic study. The early events in herpes simplex virus infection were studied by means of radio-autography. The virus was rapidly taken up by the host cells and uncoated. Viral deoxyribonucleic acid (DNA) reached the nuclear sites of replication in 15 to 30 min after infection. The viral DNA occasionally associated with chromosomes or condensed chromatin but was more frequently found to be randomly distributed. Viral progeny appeared 3 hr after infection. These particles did not show any particular spatial relationship to the parental DNA. The morphological latent period lasted 2.5 hr.


25-hydroxycholecalciferol, the active form of vitamin D--Wisconsin scores again.


Central nervous system infections of viral etiology: the changing pattern.


Specificity of natural human agglutinins against red cells modified by trypsin and other agents.


The isolation of foot-and-mouth disease virus from African buffalo (Syncerus caffer).


Renal artery stenosis and hypertension. Observations on current status of therapy from a study of 115 patients.


Further observations on hypoglycaemia in hepatocellular carcinoma.


Heat stroke in the aged.

Heat stroke in the aged.


Dynamic asymmetries in the corticosteroid feedback path and distribution-metabolism-binding elements of the adrenocortical system.


Parameters of the stimulus initiating the adrenocortical response to hemorrhage.


Interferon and cyclic-3'5'-adenosine monophosphate: potentiation of antiviral activity.


Some aspects of visual information input and transmission.


Dynamic testing and modeling of adrenocortical secretory function.


Zonal centrifugation of microsomes from rat liver: resolution of rough- and smooth-surfaced membranes.


Conformative response of xanthosine 5'-phosphate aminase.


Studies in vivo on the biosynthesis of collagen and elastin in ascorbic acid-deficient guinea pigs. 1. After the administration of labelled proline to guinea pigs deprived of ascorbic acid for 15 days, the dorsal skin was examined 5 days later in an attempt to detect the presence of hydroxyproline-deficient collagen (protocollagen). The extent of incorporation of proline into skin collagens indicated a severe impairment of collagen synthesis. 2. A comparison of proline and hydroxyproline specific radioactivities in diffusible peptides obtained by treatment with collagenase of either purified skin collagens or direct hot-trichloroacetic acid extracts of skin failed to indicate the presence of protocollagen. Possible reasons for this are discussed. 3. The incorporation results did not indicate an inability of normal collagen, i.e. collagen hydroxylated to the normal degree, to cross-link in scurvy. 4. Incorporation of labelled proline into aortic elastin isolated from the same animals did not indicate a decrease in elastin biosynthesis in ascorbic acid deficiency, beyond that attributable to the inanition accompanying the vitamin deficiency. The proline/hydroxyproline specific-radioactivity ratio in elastin from scorbutic guinea pigs was about 6:1 in contrast with the 1:1 ratio in control groups. It is concluded that the formation of elastin hydroxyproline was ascorbate-dependent and that a hydroxyproline-deficient elastin is formed and retained in scurvy. The formation of desmosines was unimpaired in scorbutic animals. 5. Studies with chick embryos confirmed the formation of elastin hydroxyproline from free proline. Incorporation of free hydroxyproline into elastin hydroxyproline was negligible. 6. Digestion of solubilized samples with collagenase indicated that the hydroxyproline in guinea-pig aortic elastin preparations was not derived from contamination by collagen. It is suggested that most if not all of the hydroxyproline in the guinea pig elastin preparations investigated can be considered an integral part of the elastin molecule.


Metabolic control and structure of glycolytic enzymes. IV. Nicotinamide-adenine dinucleotide dependent in vitro reversal of dissociation and possible in vivo control of yeast glyceraldehyde 3-phosphate dehydrogenase synthesis.


Phosphatidate biosynthesis in mitochondrial subfractions of rat liver. 1. After conventional fractionation of rat liver homogenates in 0.88m-sucrose the mitochondrial fraction was subjected to short-term water lysis followed by separation of the resulting membrane preparations. 2. Phosphatidate formation was measured in all subcellular fractions and subfractions and was compared with the distribution of succinate dehydrogenase, monoamine oxidase, rotenone-insensitive NADH cytochrome c reductase, arylsulphatase, urate oxidase, arylesterase and glucose 6-phosphatase. 3. The results obtained indicated that mitochondria were capable of synthesizing phosphatidate, though this activity was only about one-third of the total homogenate activity. 4. Mitochondrial phosphatidate formation was located predominantly in the outer mitochondrial membrane. Although this membrane preparation was found to be significantly contaminated by the microsomal fraction, this contamination was estimated to account for not more than about 20% of the total phosphatidate formation observed in preparations of outer mitochondrial membrane.


Metabolic control and structure of glycolytic enzymes. VII. Destabilization and inactivation of yeast glyceraldehyde 3-phosphate dehydrogenase by adenosine phosphates and chymotrypsin.


Visible absorption and electron spin resonance spectra of the isolated chains of human hemoglobin. Discussion of chain-mediated heme-heme interaction.


2-keto-4-hydroxybutyrate. Synthesis, chemical properties, and as a substrate for lactate dehydrogenase of rabbit muscle.


Microbial sulfolipids. 3. The disulfate of (+)-1,14-docosanediol in Ochromonas danica.


Studies on adrenal cortical cytochrome P-450. II. Effects of inhibitors of 11 beta hydroxylation on its optical and magnetic properties.


Effects of inorganic pyrophosphate on Neurospora crassa phenylalanine transfer ribonucleic acid ligase in heterologous aminoacylation reactions.


On the nature of transfer ribonucleic acid isolated from polyphenylalanyl transfer ribonucleic acid.


Poly-5-methyldeoxycyctidylic acid and some alkylamino analogs.


Preparation of the 2,4-dinitrophenyl esters of thymidine 3'-and thymidine 5'-phosphate and their use as substrates fof phosphodiesterases.


Partial purification and preliminary characterization of estrogen-binding globulins from human plasma.


Steric effects in the glyceraldehyde 3-phosphate dehydrogenase catalyzed hydrolysis of acyl phosphates. An example of substrate-induced cooperativity.


Activity of serum ribonuclease after partial hepatectomy and acute stress in the rat.


Rapidly labeled cytoplasmic RNA in normal and phytohaemagglutinin-stimulated human lymphocytes.


A simplified procedure for the assay of 5'-nucleotidase.


Specific transfer of hydrogen from estradiol to progesterone as a regulating principle for progesterone metabolism in the liver of the female rat.


Natural occurrence of a new pyridine nucleotide consisting of nicotinamide adenine dinucleotide and adenosine diphosphate ribose joined through a ribosidic link.


Preparation and physicochemical properties of human erythrocuprein.


Studies on cytochrome c peroxidase. XIV. Recombination of apoenzyme with protoheme dialkyl esters and etioheme.


Studies on cytochrome c peroxidase. XV. Comparison of manganese porphyrin-containing cytochrome c peroxidase, horseradish peroxidase, and myoglobin.


The two-step formation and inactivation of acylphosphatase by agents acting on glyceraldehyde phosphate dehydrogenase.


Ligand-induced resistance of staphylococcal nuclease and nuclease-T to proteolysis by subtilisin, alpha-chymotrypsin, and thermolysin.


The chromium, manganese, and cobalt complexes of transferrin.


The mechanism of 6-deoxyhexose synthesis. 3. Purification of deosythymidine diphosphate-glucose oxidoreductase.


The identification of cis-11,12-methylene-2-hydroxyoctadecanoic acid from Thiobacillus thiooxidans.


Adenine phosphoribosyltransferase from monkey liver. Specificity and properties.


Rajagopalan KV, Handler P: Purification and properties of inosinic acid dehydrogenase from Escherichia coli.


Enthalpy of hydrolysis of the 3' bond of adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate.


Isolation of diphosphopyridine nucleotide-dependent L-fucose dehydrogenase from pork liver.


Radioimmunoassayable ACTH in dexamethasone nonsuppressible Cushing's syndrome.


Estrogenic antagonism of metabolic effects of administered growth hormone.


Cytochromes P-450 and b5 in human liver microsomes.


Observations on cultured cells infected with rinderpest virus by means of fluorescent antibody technique.


Decreased 17-hydroxycorticosteroid and VMA excretion during sleep following glutethimide administration in man.


ACTH sensitive adenyl cyclase in bovine adrenal cortex membrane fractions.


The transfer function of frog myoneural junctions. I. The influences of fatigue, facilitation, and depression upon impulse transmission in vitro.


Influence of thiazinamium on subcellular p-nitrophenylphosphatase in rat cerebral cortex.


Equilibrium kinetic study of the mechanism of mitochondrial and supernatant malate dehydrogenases of bovine heart.


Sleep and dream studies: current state of the art.


Regulation of the biosynthesis of purine nucleotides in Schizosaccharomyces pombe. II. Kinetic studies of IMP dehydrogenase.


Rabbit intestinal glucose-6-phosphate phosphohydrolase and inorganic pyrophosphate-glucose phosphotransferase: inhibition by phlorizin.


L-Alpha,gamma-diaminobutyrate-activating enzyme from Bacillus polymyxa. Properties and distribution.


Cyclic phosphodiesterase having 3'-nucleotidase activity from Bacillus subtilis. Purification and some properties of the enzyme.


The role of essential-SH groups of yeast alcohol dehydrogenase.


Stereospecific aging of phosphonylated cholinesterases.


Transmural electrical stimulation of the ureter.


Resistance of rabbits and guinea pigs to the diabetogenic effect of streptozotocin.


Early insulin synergistic activity of growth hormone.


Characteristics of family caretakers.

Characteristics of family caretakers.


Reactivity of plasmalogens: kinetics of acid-catalyzed hydrolysis. The acid-catalyzed hydrolysis of the alpha,-unsaturated ether group of two plasmalogens, lysophosphatidal choline and lysophosphatidal ethanolamine, and several model compounds (isobutyl vinyl ether, 1-butenyl ethyl ether, and dihydropyran) was studied by determining the true second-order rate constants. The results indicate that the chemical reactivity of the substituted vinyl ether group in plasmalogens is not appreciably affected by the presence of a bulky substituent on the -carbon. Activation energies, enthalpies, and entropies were also determined (from measurements of the rate constants at different temperatures).


Isolation and identification of phospholipids of bovine rhodopsin. Phospholipids present in digitonin solutions of bovine rhodopsin have been identified and assayed. Digitonin interferes with extraction of lipids by the usual methods; digitonin was therefore removed from the preparation as an ergosterol digitonide, soluble in absolute ethanol but precipitated in 80% ethanol. The supernatant 80% ethanol contained one portion of the phospholipid, mainly choline and ethanolamine phosphoglycerides with traces of serine phosphoglyceride and sphingomyelin. The rhodopsin residue (free from digitonin) was extracted with chloroform-methanol 2:1; this extract contained the rest of the phospholipid, which consisted only of choline and ethanolamine phosphoglycerides. Plasmalogens were not found, but could have decomposed during the procedures.


Synthesis of lipids from acetate by human preputial and abdominal skin in vitro. Lipogenesis in vitro from acetate-1-(14)C was studied in human preputial skin and abdominal skin. Radioactive lipids were separated by column chromatography on Florisil and by thin-layer chromatography on silica gel. Radioactivity was incorporated chiefly into the triglyceride, sterol, and polar lipid fractions, while lesser amounts of (14)C were found in the hydrocarbon, wax, diglyceride, monoglyceride, and fatty acid fractions; labeling of steryl esters was minimal. On thin-layer chromatography, the radioactive polar lipids had mobilities similar to lysolecithin, phosphatidyl choline, phosphatidyl ethanolamine, and phosphatidic acid. The radioactive fatty acids of the different lipid fractions were separated by gas-liquid chromatography. The major (14)C-labeled acids were 16:0 and 18:0. Radioactivity was also detected in acids 14:0, 15:0, 16:1, 18:1, 18:2, 20:0, 20:1, 22:0, 24:0, 24:1, and 26:0. No radioactivity could be detected in arachidonic acid, although this fatty acid comprises 9% of the chromatographed fatty acids. The pattern of incorporated (14)C was different from the percentage mass composition of the fatty acids. Skin is therefore active in the biosynthesis of a wider variety of lipids than previously demonstrated.


Tissue distribution of glycosphingolipids in a case of Fabry's disease. A survey was made of the glycolipid composition of various tissues, including liver, spleen, kidney (cortex and medulla), lymph node, pancreas, prostate gland, heart muscle, thenar muscle, gastrointestinal smooth muscle, frontal cerebral cortex, anterior thalamus, brain stem, a peripheral autonomic ganglion, and renal arterial intima and media, from a patient who died with Fabry's disease. The tissues had been fixed in formalin for 3 yr. Analytical data on trihexosyl ceramide from heart muscle and pancreas indicate a structure identical to trihexosyl ceramide from kidney: galactosylgalactosylglucosyl ceramide. Fatty acid compositions of trihexosyl ceramide and dihexosyl ceramide revealed a wide range of fatty acids, with 16:0, 18:0, 20:0, 22:0, 24:0, and 24:1 predominating. These glycolipids comprised 10-41% of the total lipid in the formalin-fixed organs studied. Trihexosyl ceramide predominated in all tissues and was the only glycolipid found in muscle tissues, lymph node, and arterial tissues. Dihexosyl ceramide was found in kidney, pancreas, liver, spleen, and cerebral tissues. The accumulation of trihexosyl ceramide in cardiac muscle and arterial tissues may account in part for the cardiovascular complications so prominent in Fabry's disease.


Identification of ceramide phosphorylethanolamine and ceramide phosphorylglycerol in the lipids of an anaerobic bacterium. Nearly half the phospholipids isolated from the anerobic bacterium Bacteroides melaninogenicus are phosphosphingolipids. The two major phosphosphingolipids have been characterized as ceramide phosphorylethanolamine and ceramide phosphorylglycerol. The long-chain bases of these phosphosphingolipids appear to have branched and normal saturated carbon chains of 17, 18, and 19 atoms; the phosphate is at the 1-position of the long-chain base. The composition of the amide-linked fatty acids of the phosphosphingolipids differs from that of the ester-linked fatty acids of the diacylphosphoglycerides in having a higher percentage of 14:0, 17:0, and 18:0 acids as well as containing nearly all the monoenoic fatty acids found in the bacterial lipids. The finding of phosphosphingolipids in bacteria is exceedingly rare and to our knowledge ceramide phosphorylglycerol has not been previously found in nature.


Determination of individual conjugated bile acids in human bile. A method has been developed and validated for the determination of the six major conjugated bile acids, cholesterol, and total phospholipids in bile of human subjects previously injected with 4-(14)C-cholesterol. The procedure is designed for use with 5-10 ml of duodenal or T-tube bile and eliminates difficulties associated with existing methods for bile acid determination, in particular the requirement for preliminary saponification under pressure or the use of paper chromatography. Saponification under pressure is employed only in steps where partial destruction of the steroid moiety of conjugated bile acids is not a crucial matter. A preliminary Folch extraction and washing step separated free cholesterol and phospholipids (bottom layer) from the six major conjugated bile acids (top layer). The conjugated bile acids were then fractionated cleanly by thin-layer chromatography to give four groups, the (14)C content of each of which was determined. A second aliquot of the top layer was used to determine (after deconjugation) the radioactivity ratio of deoxycholic acid to chenodeoxycholic acid for the two unresolved groups (dihydroxycholanoic acid conjugates with glycine and taurine, respectively). A third aliquot was used for determination of specific activities of the methyl esters of cholic, chenodeoxycholic, and deoxycholic acids derived from the total bile salts. Appropriate calculations yielded the concentration in bile of all six major bile acid conjugates.


Composition of alkoxylipids of human heart and aorta. The major alkoxylipids of human heart and aorta are alkyl and alk-1-enyl diacyl glycerols, alkyl acyl and alk-1-enyl acyl glycerophosphoryl cholines, and the corresponding glycerophosphoryl ethanolamines. There are no pronounced differences in the composition of corresponding classes of alkoxylipids from heart, aorta, and other human tissues previously reported.


Minor footnote to Michigan Medical History: the Lansing strain of poliomyelitis virus.


Review of virus respiratory infections in the Milwaukee area, 1955-65.


Conjugation of androsterone by the guinea-pig kidney in vitro. Radioactive androsterone was incubated with kidney slices from guinea pigs in a Ringer bicarbonate buffer. For analysis, the radioactivity was partitioned between chloroform and water for the separation of non-polar and polar steroids. After multiple chromatography procedures, the non-polar fraction was shown to contain androsterone and at least four other similar steroids, not identified in this study. The polar fraction yielded numerous conjugates from which androsterone glucuronide and androsterone sulphate in approximately equal quantities were isolated and characterized.


The presence of the adult-type pattern of adrenal steroids in the one day old rat.


Phosphoenolpyruvate carboxytransphosphorylase. 3. Comparison of the fixation of carbon dioxide and the conversion of phosphenolpyruvate and phosphate into pyruvate and pyrophosphate.


Phosphoenolpyruvate carboxytransphosphorylase. V. Mechanism of the reaction and the role of metal ions.


Spin-label studies of glycerinated muscle fibers.


Sequential degradation of nucleic acids. Degradation of Escherichia coli B phenylalanine transfer ribonucleic acid.


Pentose synthesis in Escherichia coli.

Pentose synthesis in Escherichia coli.


The oxidation of thioethers by bromine. A model system for oxidative phosphorylation.


The role of G factor in protein synthesis. Studies on a temperature-sensitive Escherichia coli mutant with an altered G factor.


The pathways of corticosteroid biosynthesis by homogenates of adrenal tissue from rabbits stimulated with adrenocorticotropin.


Ligand interactions with hemoprotein P-450. II. Influence of phenobarbital and methylcholanthrene induction processes on P-450 spectra.


Chemical synthesis of three deoxyribododecanucleotide chains of defined sequence.


The biosynthesis of bacteriochlorophyll. The characterization of latter stage intermediates from mutants of Rhodopseudomonas spheroides.


The conformation of angiotensin II in aqueous solution. II. Dialysis and gel filtration behavior of [Asn 1-Val 5]-angiotensin II.


Postoperative gas gangrene.

Postoperative gas gangrene.


Postoperative clostridial infections in Britain. Eighty-five cases of clostridial infection, including 56 cases of gas gangrene, followed "clean" surgical operations performed in British hospitals in the course of two years. Nearly all the serious infections followed amputations of the leg for ischaemia or other operations on the leg in which a foreign body was implanted. All the infections were sporadic, and the evidence suggested that the infecting organism usually came from the bowel of the patient. Nearly half of the operations were performed in modern theatres with satisfactory ventilation and unexceptionable arrangements for the sterilization of instruments and dressings.Skin sterilization was often carried out perfunctorily or with agents with poor sporicidal activity. Total eradication of spores from the skin is, however, difficult to achieve, and recontamination may occur during the operation. Hence it is considered justifiable to give penicillin prophylactically to the small group of patients at serious risk from postoperative gas gangrene.


On the mechanism of the enzymatic conversn of cholest-5-ene-3-beta, 7-alpha-diol into 7-alpha-hydroxycholest-4-en-3-one.


Drugs and dreams, a synthesis.

Drugs and dreams, a synthesis.


Electron histochemical observation of succinic dehydrogenase activity in various parts of neurons.


ACTH-excess and increased urinary pressor activity.


Some considerations of motor and electrical acitivity in states of diminished awareness.


Susceptibility of various strains of chicken to the oncogenicity of Yaba virus.


Preparation of radioactively labeled monodisperse silica spheres of colloidal size.


Traditional healers and their female clients: an aspect of Nigerian sickness behavior.


The hierarchy of resort in curative practices: the Admiralty Islands, Melanesia.


Urban illness: physicians, curers and dual use in Bogota.


Effect of nitrofurazone on the aerobic metabolism of uniformly labelled [14C]glucose in tissue slices of rat testes.


Tinel's percussion test.

Tinel's percussion test.


Specific inhibition of an antibody response by affinity labelling.


In vivo studies of the FBJ murine osteosarcoma virus.


Treatment of Cushing's disease. Correction of hypercortisolism by o,p'DDD without induction of aldosterone deficiency.


Catecholamine-secreting paraganglioma of glomus-jugulare region resembling pheochromocytoma.


Persistence of foot-and-mouth disease virus in lymph nodes and blood of experimentally infected buffalo-calves in West Pakistan.


Respiratory infection of lambs with an ovine strain parainfluenza virus type 3.


Hereditary deafness in man.

Hereditary deafness in man.


Hormonal pattern in bronchial asthma (reversible obstructive lung disease). 1. Role of adrenal steroids.


Effect on the lungs and their regional lymph nodes of BCG and quartz dust. An experimental study on rats.


Effect of BCG on translocation of quartz dust from the lungs to their regional lymph nodes. An experimental study on rats.


Energy flux and membrane synthesis in photosynthetic bacteria. Synthesis of the energy-converting membrane complex of the photosynthetic purple bacterium Rhodopseudomonas capsulata during growth under different conditions of energy flux was studied by examining the disorganizing effects of polymyxin B, with or without lysozyme, on integrity of the cell envelope. Cells growing with a limited supply of energy show an elevated bacteriochlorophyll content and increased resistance to breakdown of the "permeability barrier" by these agents. It seems that purple bacteria respond to energy restriction by preferentially synthesizing excess bacteriochlorophyll-membrane which, in effect, toughens the cell integument.


The problem of the chronic excretor of tubercle bacilli.


Clinical epidemiology of leukemia.

Clinical epidemiology of leukemia.


Exposure of vaccinated bulls and  steers to airborne infection with foot-and-mouth disease.


Visceral and behavioral responses to intraduodenal fat. Introduction of milk or corn oil into the duodenum of the cat evokes an increase in superior mesenteric blood flow (blocked by atropine), an inhibition of gastric and duodenal motility, and sedation. Cholecystokinin-pancreozymin mimics the mesenteric vascular effect of intraduodenal fat and seems to have a sedating action.


Natural occurrence and experimental study of pox and Haemoproteus infections in a mute swan (Cygnus olor).


Scintillation counting of 14CO2 from in vitro systems: a comparison of some trapping agents.


Liquid scintillation radioassay of thin-layer chromatograms: problems in the use of external standard quench correction.


Porphyrins derived from the prosthetic group of myeloperoxidase.


The mechanism of cycloheximide inhibition of protein synthesis in rabbit reticulocytes.


Cell wall composition and protoplast formation of some Oomycete species.


Phenoxy radical intermediates in the enzymatic degradation of lignin model compounds.


The regulation of glycolate metabolism in Chlamydomonas reinhardtii.


Rapid resolution of flavin-adenine dinucleotide and its mononucleotides on cellulose layers using aqueous ammonium salts and formic acid.


Brain monoamines and adrenocortical activation. 1. The effect of a pharmacologically induced increase or depletion of brain monoamines (5-hydroxytryptamine, noradrenaline, dopamine) was investigated with respect to adrenocortical function.2. No strict correlation was found between the depletion of brain amine stores induced by prenylamine or p-chlorophenylalanine and adrenocortical activation, even at times in which the peak effect of brain amine depletion occurs.3. Restraint stress causes a manifest increase in brain 5-hydroxytryptamine while decreasing the cerebral noradrenaline and dopamine content. This stress strongly stimulates corticosterone secretion by the adrenals.4. A monoamine oxidase inhibitor, nialamide, at a dose which causes an evident increase in brain amine concentrations, does not modify plasma corticosterone. At the same dose it was, furthermore, unable to prevent the adrenocortical stimulation induced by restraint stress.5. Brain amine content does not seem to play an important part in the control of corticotrophin releasing factor in corticotrophin secretion by the pituitary gland. The relationship between hypothalamic monoamines and other neurohumours is discussed.


The mechanism of plateau formation by anemone toxin.


Lithium and the electroencephalogram during sleep.


Evaluation of metopiron and ACTH tests in prolonged corticosteroid treatment of chronic asthma.


Adjuvant properties of alginate in bacterial, viral and protein vaccines.


The genesis of dermatoglyphics.

The genesis of dermatoglyphics.


Fifty-two forms of childhood cancer: United States mortality experience, 1960-1966.


Biological effects of ultrasound: non-toxicity to poliovirus.


Thermoregulation in the rat: interactions of cholinergic and adrenergic agents in the hypothalamus.


Some guanidine esters and their possible relationship to the action of tetrodotoxin.


Studies on the relation of melatonin to sleep.


The pharmacology of 2-imino-5-benzoyloxy-hexahydropyrimidine.


Effect of EDTA and other chelating agents on norepinephrine uptake by rabbit aorta in vitro.


Pharmacological and biochemical correlates of 5-hydroxytryptamine entry into the central nervous system during maturation.


NAD effects on the biochemistry and psychological performance of alcoholics under ethanol stress.


Sarcoma of the stomach: a review of 39 cases.


Effect of maternal antibody on Marek's disease.


Buffalopox virus.

Buffalopox virus.


The active chemical state of D-glyceraldehyde 3-phosphate in its reactions with D-glyceraldehyde 3-phosphate dehydrogenase, aldolase and triose phosphate isomerase. Glyceraldehyde 3-phosphate exists as the geminal diol and the free aldehyde in the molar ratio 29:1 in aqueous solution. The rate constant of the conversion of diol into aldehyde is 8.7x10(-2)sec.(-1) in the pH range 7.3-8.6 at 20 degrees . The free aldehyde is the substrate for d-glyceraldehyde 3-phosphate dehydrogenase. Over a wide concentration range of enzyme the rate of conversion of diol into aldehyde is the rate-limiting process in the catalytic oxidation of d-glyceraldehyde 3-phosphate by NAD(+). Aldolase and triose phosphate isomerase both liberate d-glyceraldehyde 3-phosphate as the aldehyde. This suggests that the relatively slow diol-aldehyde interconversion does not restrict the rate of glycolysis.


Purification of a trifunctional enzyme, catalysing three steps of the histidine pathway, from Neurospora crassa. 1. A procedure is described for the purification of an enzyme from Neurospora crassa that has three catalytic functions. These are 1-N-(5'-phosphoribosyl)-ATP pyrophosphohydrolase, 1-N-(5'-phosphoribosyl)-AMP cyclohydrolase and histidinol dehydrogenase (l-histidinol-NAD oxidoreductase, EC 1.1.1.23), and are responsible for the catalysis of reactions 2, 3 and 10 in the histidine pathway. The ratio of these three catalytic activities remains approximately the same throughout the purification procedure. Evidence is presented that the purified preparations contain a single protein exhibiting association-dissociation equilibria.


Amino acid uptake in isolated chick embryo heart cells. 1. The preparation of cell suspensions by treatment of chick embryo hearts with collagenase at various stages of development is described. 2. Measurements of oxygen consumption, incorporation of labelled leucine into protein and accumulation of labelled alpha-aminoisobutyric acid against a concentration gradient indicated a long-lasting viability of the isolated heart cells in vitro; a satisfactory preservation of subcellular structures, including plasma membrane, was assessed by electron-microscopic examination. 3. The rate of alpha-aminoisobutyric acid accumulation by cardiac cells isolated from hearts at different stages of embryological development decreased with aging; insulin stimulated the intracellular accumulation of this amino acid analogue. 4. Insulin increased the uptake by isolated heart cells of several (14)C-labelled naturally occurring amino acids; however, the fraction of amino acid taken up by the cells that was recovered free intracellularly, and therefore the concentration ratio (between intracellular water and medium), was enhanced by the hormone only with glycine, proline, serine, threonine, histidine and methionine. When isolated heart cells were incubated in the presence of a mixture of labelled amino acids, the addition of insulin increased the disappearance of radioactivity from the medium. 5. The general pattern of amino acid transport (in the absence and in the presence of insulin) in isolated cardiac cells was similar to that found in intact hearts, suggesting that the biological preparation described in this paper might be useful for studies of cell permeability and insulin action.


Isomers of glucosaminylphospatiylglycerol in Bacillus megaterium. 1. The lipids of Bacillus megaterium were extracted and three lipids containing glucosamine were identified. One of these is not a phospholipid, but the other two, which differ in their chromatographic behaviour, contain phosphorus, glycerol, fatty acid and d-glucosamine in the molar proportions 1:2:2:1. 2. In both phosphoglycolipids, the fatty acids are bound in ester linkage, and both yield 2,5-anhydromannose and 3-sn-phosphatidyl-1'-sn-glycerol on treatment with sodium nitrite. 3. Both phosphoglycolipids were N-acetylated and, after removal of fatty acids by mild alkaline hydrolysis, in both cases N-acetylglucosamine was quantitatively released by beta-N-acetylhexosaminidase. 4. The glucosaminylglycerols derived from the two phosphoglycolipids by partial acid hydrolysis differ in their behaviour towards periodate. In one case 1 mole of periodate is rapidly consumed/mole of glucosaminylglycerol, but in the other case under identical conditions the consumption of periodate is negligible. 5. The phosphoglycolipids were identified as 1'-(1,2-diacyl-sn-glycero-3-phosphoryl)-3'-O-beta-(2-amino-2-deoxy-d-glucopyranosyl)-sn-glycerol and as 1'-(1,2-diacyl-sn-glycero-3-phosphoryl)-2'-O-beta-(2-amino-2-deoxy-d-glucopyranosyl)-sn-glycerol. 6. Both phosphoglycolipids are good substrates for phospholipase A: neither is a substrate for phospholipase C from Clostridium perfringens, and only the 3'-glucosaminide is a substrate for phospholipase D.


Presence, induction and possible role of glucose 6-phosphatase in mammalian pancreatic islets. 1. Pancreatic islets from several mammalian species were investigated for hydrolytic activity towards glucose 6-phosphate. Both the total phosphatase activity towards this substrate and the proportion cleaving glucose 6-phosphate in preference to beta-glycerophosphate varied widely between species. In pancreatic-islet homogenates prepared from mice and guinea pigs there was a higher rate of liberation of P(i) at pH6.7 from glucose 6-phosphate than from beta-glycerophosphate. In these two species cortisone treatment enhanced the enzyme activity towards glucose 6-phosphate but not that towards beta-glycerophosphate. Simultaneous injections of ethionine or puromycin blocked this stimulating effect of cortisone. 2. With whole homogenates of mouse pancreatic islets, inverse plots of the relationship between glucose 6-phosphate concentration and enzyme activity suggested the simultaneous action of two enzymes with different K(m) values. After fractionation of islets from obese-hyperglycaemic mice by differential centrifugation, one of these enzymes could be shown to be localized in the microsome fraction. It had K(m) for glucose 6-phosphate about 0.5mm and optimum pH6.7. It split glucose 6-phosphate in preference to beta-glycerophosphate, glucose 1-phosphate, fructose 6-phosphate and fructose 1,6-diphosphate. Incubation of the microsomes at pH5.0 and 37 degrees for 15min. decreased the enzyme activity by about 80%. Glucose was a potent inhibitor, the type of inhibition being neither strictly competitive nor non-competitive. It is suggested that the results indicate the presence of glucose 6-phosphatase in mammalian endocrine pancreas, and that this enzyme may play a role in the metabolic regulation of release of insulin.


1-Anilinonaphthalene-8-sulphonate, a fluorescent conformational probe for glutamate dehydrogenase. 1. The interaction of 1-anilinonaphthalene-8-sulphonate with ox liver glutamate dehydrogenase was examined. 2. The fluorescence of the dye is enhanced 100-fold on binding. 3. A further enhancement is observed when NADH and GTP are added to the enzyme. 4. By using this property of the dye to measure conformational equilibria in the enzyme the effects of coenzyme, inhibitors, enzyme concentration, ionic strength and pH on the allosteric transitions were studied. 5. GTP and NADH interact with the enzyme in a heterotropic manner. 6. The rate of the structural transition brought about by GTP and NADH is biphasic with half-lives of 34 and 200msec. 7. The relation of these observations to regulatory mechanisms is discussed.


A survey of sheep sera for antibodies to an ovine strain of parainfluenza 3 virus.


The isolation of a basic coupling factor from preparations of factor F3.


Serological findings in bovine herpes mammillitis.


Tetrazolium salt reduction studies in slide histochemistry. I. Observations on the reductase activity of experimental tumours implanted in the mouse brain and liver, as measured by optical densitometry.


Tetrazolum salt reduction studies in slide histochemistry. II. A quantitative study in two experimental tumour models.


Detection and separation of aliphatic polycarboxylic acids by reversed-phase partition thin-layer chromatography.


Pelvic arteography in malignant trophoblastic neoplasia.


5-Phosphoribosyl pyrophosphate synthetase from Ehrlich ascites tumor cells.


Transfer areas and clean zones in operating suites.


The isolation of parainfluenza 4 subtypes A and B in England and serological studies of their prevalence.


Contribution on the etiology of acute stenosing laryngotracheobronchitin in children.


Measurement of reticuloendothelial system phagocytosis and catabolism: a new method.


Lysosomal enzyme release in mouse strains susceptible and resistant to antigen-antibody complex induced anaphylactic shock.


Effects of cholera on the human hypothalamus and hypophysis. II.


Bacteriophage sigma factor for RNA polymerase.


Interactions of proteins and lipids: structure and polymorphism of protein-lipid-water phases.


Does strychnine block inhibition post-synaptically?


Encoding of nerve signals from retinal rods.


Metabolic and morphologic studies of hepatocarcinogenesis in the rat. ANL-7535.


Survival of mice under daily gamma irradiation: progress report for incidence of some tumors. ANL-7535.


Effect of rapid eye movement (dreaming) sleep deprivation on retention of avoidance learning in rats. Rep No 563.


Phosphohydrolase activity of a Bacillus species.


Experimental study on atherosclerosis--an attempt at its prevention and treatment.


Calcified carcinoma of the colon superimposed on chronic ulcerative colitis.


Histochemical studies of alkaline phosphatase in carcinoma of the liver.


Glutaraldehyde-treated cells in the indirect hemagglutination tests for amebiasis.


Congenital cytomegalovirus infection.

Congenital cytomegalovirus infection.


Indirect hemagglutination and ameba-immobilization tests and their evaluation in intestinal and extraintestinal amebiasis.


Hydrolytic enzymes in the neonatal rat lung.


Enzyme suppression by Mycobacterium bovis.


Studies on the location of sulfhydryl groups in erythrocyte membranes with magnetic resonance spin probes.


Sarcoplasmic reticulum. 8. Use of 8-anilino-1-naphthalene sulfonate as conformational probe on biological membranes.


ESR probing of macromolecules: function and operation of structural units within the active site of alpha-chymotrypsin.


Altered properties of thymidine kinase induced in hamster kidney cells by adenovirus types 5 and 12.


Reversed energy conversion reactions of bacterial photophosphorylation.


Effect of substrates on reconstitution of the mitochondrial respiratory chain under various conditions.


A nitrite reductase with cytochrome oxidase activity from Micrococcus denitrificans.


Purification and properties of cytochromes c of Azotobacter vinelandii.


Electron spin resonance studies of free radicals derived from plastoquinone, alpha- and gamma-tocopherol and their relation to free radicals observed in photosynthetic materials.


The isolation of estrone sulfate and estradiol-17 beta sulfate from stallion testes.


Virus particles in epidermodysplasia verruciformis with carcinoma.


The effect of luteinizing hormone and adenosine 3',5'-monophosphate on progesterone biosynthesis by bovine granulosa cells in culture.


Transformation of cells cultured from human brain tissue.


Studies on polymer of adenosine diphosphate ribose. VI. Radioautographic demonstration of incorporation of NAD into nuclear macromolecule.


Transplantation of spontaneous hepatomas in C3H mice; biological and biochemical studies.


Modes of action of three bacteriostatic agents in their inhibitory effect on induction of hepatoma in rats fed 4-(dimethylamono)-azobenzene. I. Effect on metabolic fate of 4-(dimethylamino)azobenzene.


Immunological studies on in vivo liver catalase-depressing substance.


Interference of leukemogenesis by mammary tumorigenesis in a colony of AKR mice.


In vitro culture of human cancer cells for cancer chemotherapy. II. In vitro drug sensitivity of cultured suspended cell line of gastric carcinoma origin.


Diagnosis and management of mediastinal masses in children.


Effect of histological fixation on carbohydrate-rich tissue compounds and their periodate consumption.


Acid angiotensinase as a lysosomal enzyme.


Centrally mediated vasopressor effect of angiotensin II in man.


Responses of the cardiac sympathetic receptors to various substances.


Heat resistance and alpha-toxigenicity of Clostridium perfringens strains in normal intestines of Japanese.


Ibaraki virus, an agent of epizootic disease of cattle resembling bluetongue. I. Epidemiologic, clinical and pathologic observations and experimental transmission to calves.


Ibaraki virus, an agent of epizootic disease of cattle resembling bluetongue. II. Isolation of the virus in bovine cell culture.


Incorporation of 32P into phospholipids of influenza A and parainfluenza 1 viruses.


Hydrocephalus in hamsters, ferrets, rats, and mice following inoculations with reovirus type I. I. Virologic studies.


Hydrocephalus in hamsters, ferrets, rats, and mice following inoculations with reovirus type I. II. Pathologic studies.


The fine structure of brown adipose tissue. 3. The effect of cold exposure and its mediation in newborn rats.


Atypical peripheral (bronchiolar) adenoma of the lung.


Clinical observations on cancer patterns at the non-white hospital Baragwanath, Johannesburg, 1948-1964.


Improved method for measurement of renin activity in human plasma.


The neurologist's view of radiation therapy of nervous tissue.


Alterations of some proteosynthetic  reactions in the course of immunisation with heterologous proteins.


The virus watch program: a continuing surveillance of viral infections in metropolitan New York families. 8.


Ecologic studies of vesicular stomatitis virus. I. Prevalence of infection among animals and humans living in an area of endemic VSV activity.


Positional distribution of fatty acids in brain polyphosphoinositides.


Treatment of adenovirus infection of the eye with 5-iodo-2'-deoxyuridine. A double-blind trial.


Dextrochrysin, a new antibiotic.

Dextrochrysin, a new antibiotic.


Relief of uremic polyneuropathy after bilateral nephrectomy.


Fate of angiotensin I and II in the human pulmonary circulation.


Treatment of infectious granulomas of the liver and infectious mononucleosis. Treatment of hepatic schistosomiasis, tuberculosis, syphilis and echinococcus cyst.


Plain film roentgen diagnosis of amebic hepatic abscess.


Functional hepatoma demonstrated with rose bengal scanning.


Recent advances in pediatric surgery.

Recent advances in pediatric surgery.


Some recent advances in endocrine surgery.


Clinical advances in the treatment of cancer.


Progress in abdominal surgery.

Progress in abdominal surgery.


Tumors of the salivary glands.

Tumors of the salivary glands.


Effect of morphine on cerebral glycolytic intermediates and enzymes of rats in vitro.


Comparison of the biochemical alterations elicited in livers from rats treated with carbon tetrachloride, chloroform, 1,1,2-trichloroethane and 1,1,1-trichloroethane.


Identification of somatotropin as the hormone in a mixture of somatotropin, adrenocorticotropic hormone and prolactin which decreased liver drug metabolism in the rat.


Serological studies in infectious mononucleosis. Serological investigations performed on 27 patients with illnesses resembling infectious mononucleosis showed a significant increase in high antibody titres (more than 1:40) to EB virus in 11 of the 12 who developed heterophile antibodies. Two of these patients, however, had a significant increase in antibody titre to cytomegalovirus and rubella virus, respectively. Of 15 patients who failed to develop heterophile antibodies, one had a high antibody titre to EB virus, the others generally having undetectable or low antibody titres. The insidious onset of the illness in many patients together with the fact that EB virus antibodies rose to high titres rapidly reduced the value of this investigation diagnostically.EB virus antibody was still present in the sera of five patients who had had well-authenticated heterophile-antibody-positive infectious mononucleosis some four to seven years previously. Twenty-seven out of 70 (39%) healthy nurses had antibody at a level of more than 1:10 to EB virus. The presence of EB virus antibody in different population groups appears to be related to such factors as age and socioeconomic status.


Weak ankles. A study of common peroneal entrapment neuropathy. Twenty-three patients were seen with entrapment neuropathy in a two-and-a-half-year period. Symptoms consisted of pain, paresis, and paraesthesia in the distribution of the common peroneal nerve. Some degree of paresis was often present, which in five patients was severe enough to cause drop foot. In 20 patients decompression of the entrapped nerve at the neck of the fibula was quickly and completely successful. It is suggested that the ankle weakness which frequently follows sprains and other forced inversion injuries may often be at least partially due to entrapment of the common peroneal nerve.


A tetrodotoxin-resistant primary afferent depolarization.


Sleep of unrestrained chimpanzee: cortical and subcortical recordings.


Pyramidal excitation of lemniscal neurons and facilitation of sensory transmission in the spinal trigeminal nucleus of the cat.


An electroencephalographic study of nocturnal sleep in temporal lobe epilepsy.


Effect of diazonium derivatives on myosin A adenosine triphosphatase. II. A possible conformational change induced by ATP.


The isolation of cytopathogenic enteroviruses from the sewage in Moscow and the Moscow district in 1963-1964. 2. The efficacy of purification of sewage from cytopathogenic enteroviruses.


Vascular invasion by glioma cells in man: an electron microscopic study.


"Ephphatha, or, some advice to stammerers".


Computer-generated plots of results of antimicrobial-susceptibility tests.


Immunization of mice against Ehrlich ascites tumour using a hamster-Ehrlich ascites tumour hybrid cell line.


The metabolic and endocrine response to acute medical stress.


Treatment of ovarian carcinoma: surgical aspects.


Effects of drugs on placental function and their relation to congenital abnormalities.


The effect of small doses of human ACTH on serum corticosteroid levels in man.


Skin, liver, and corneal lesions produced by injections of high infectious titer and UV-irradiated preparations of herpes simplex virus in rabbits and guinea pigs.


The effect of dextrans on zeta potential.


Enhanced virus yields and decreased interferon production in mycoplasma-infected hamster cells.


Serum immunoglobulins in multiple sclerosis patients.


The effect of proinsulin on the immunoassay of insulin and its possible relation to states of hyperinsulinemia.


Resistance to virus challenge in mice infected with protozoa or bacteria.


Purine analogs as potential anticytomegalovirus agents.


Adrenal response to hypophysectomy in the newborn dog.


The pathology of experimental infectious canine hepatitis in neonatal puppies.


Adrenal response to excess corticotropin in coronary-prone men.


Observations on the histopathology of bovine herpes mammillitis.


Human diploid cell transformation by DNA extracted from the tumor virus SV40. The DNA isolated from simian virus 40 (SV40) can transform human fibroblast cells in tissue culture. Clonal lines of DNA-transformed human cells have been obtained that have properties characteristic of cells transformed by whole virus. They all contain SV40 T-antigen, and infectious virus can be recovered by cocultivation. This is the first demonstration of a permanent genetic alteration produced in human cells by purified DNA.


Nursing care approaches for operant reinforcement with psychiatric patients.


Going home.

Going home.


Studies in situ on the neuroendocrine system of the giant water bug, Belostoma indica (Lep. and Serv.) (Heteroptera: Belostomatidae).


Therapy of being-with: reentrance to existence.


Activation of cardiac glycogen phosphorylase by calcium.


Influence of prostaglandin E1 on the terminal vascular bed. Experiments were performed to explore the influence of (PGE1) prostagandin E1 on the local regulation of blood flow in the terminal vascular bed of the rat mesoappendix.  The vasomotor activity of PGE1 on specific vascular components of the microcirculation as well as its effects on vascular responsiveness to locally applied vasoconstrictor and vasodilator agents were documented by direct in vivo microscopic observation.  Locally administered PGE1 transiently dilated all muscular microvessels.  Unlike the other naturally occurring vasodilator agents such as bradykinin and histamine, PGE1 antagonized the constrictor action of angiotensin, epinephrine, norepinephrine, and vasopressin long after its vasodilator activity had vanished.  However, PGE1 did not interfere with the constrictor activity of serotonin, nor did it alter vascular responsiveness to the dilators, bradykinin, and histamine.  Findings suggest that PGE1 may serve as a local hormonal regulator of blood flow in the microcirculation by virtue of its vasodilator properties and its ability to suppress vascular responsiveness to endogenous constrictor amines and polypeptides.


Morphological description of cell cultures containg a Type-II (Marek's) leukosis virus (JM strain).


An adjunct to training psychiatric acids in behavioral modification techniques.


Psychiatric nursing in a unique environment.


Health systems in the socialized countries.


Bioassay of cell cultures containing a Type-II leukosis virus (JM strain).


Chicken embryo lethal orphan virus: virus localization and histopathology in chicken embryos.


Studies on acute marek's disease. XI. propagation of the GA isolate of marek's disease in tissue culture.


The fibrogenic action of silica.

The fibrogenic action of silica.


Geriatrics (automobile driving).

Geriatrics (automobile driving).


Group treatment for handicapped children.


Toward a greater understanding of mutual withdrawal in a psychiatric setting.


Persistence of the steroidogenic effect of adenosin-3',5'-monophosphate in vitro: evidence for a third factor during the steroidogenic effect of ACTH.


Fetal secretion of glucocorticoids.

Fetal secretion of glucocorticoids.


Molecular states of cytochrome oxidase.

Molecular states of cytochrome oxidase.


Psychiatric drugs & trends. The bitter and the sweet of sweeteners.


Speaking out on: caring plus standards.

Speaking out on: caring plus standards.


Fine structure of Sendai virus nucleocapsid.


Course of air-borne infection cause by B virus (Herpesvirus simiae) II. Distribution of B virus in the organism of rabbits and histopathological changes in the organs.


Angiotensin II analogs. I. Synthesis and biological evaluation of [Gly-1,Gly-2,Ile-5]-angiotensin II, [Ac-Gly-1,Gly-2,Ile-5]-angiotensin II, and [Gly-1,Gly-2,Ile-5,His(Bzl)-6]-angiotensin II.


Angiotensin II analogs. II. Cyclo-(-Val-Tyr-Ile-His-Pro-Phe-).


Progress at home with a mentally retarded child.


Serological epidemiology of reovirus infection. 1.


Trigeminal sensory neuropathy.

Trigeminal sensory neuropathy.


The cranial mononeuropathies.

The cranial mononeuropathies.


Effect of glutethimide and aminoglutethimide on the pituitary-adrenal system.


Relief of decerebrate rigidity by viscero-somatic reflex action.


Safety in a pharmacology laboratory.

Safety in a pharmacology laboratory.


Iontophoretic application of acetylcholine to thermoregulatory centers of rats.


A comparative evaluation of metronidazole and other amoebicidal drugs on the strains of Entamoeba histolytica.


Serum electrophoretic patterns in angiography.


Modification of insulin response and alloxan diabetes by hypothalamic lesions in rats.


Three cases of hepatocellular carcinoma with unusual presentation.


Lipolytic activity of ACTH and its fragments on isolated fat cells of the rat: inhibition by different types of antisera.


Distribution of enzymes in mesophyll and parenchyma-sheath chloroplasts of maize leaves in relation to the C4-dicarboxylic acid pathway of photosynthesis. 1. Mesophyll and parenchyma-sheath chloroplasts of maize leaves were separated by density fractionation in non-aqueous media. 2. An investigation of the distribution of photosynthetic enzymes indicated that the mesophyll chloroplasts probably contain the entire leaf complement of pyruvate,P(i) dikinase, NADP-specific malate dehydrogenase, glycerate kinase and nitrite reductase and most of the adenylate kinase and pyrophosphatase. The fractionation pattern of phosphopyruvate carboxylase suggested that this enzyme may be associated with the bounding membrane of mesophyll chloroplasts. 3. Ribulose diphosphate carboxylase, ribose phosphate isomerase, phosphoribulokinase, fructose diphosphate aldolase, alkaline fructose diphosphatase and NADP-specific ;malic' enzyme appear to be wholly localized in the parenchyma-sheath chloroplasts. Phosphoglycerate kinase and NADP-specific glyceraldehyde phosphate dehydrogenase, on the other hand, are distributed approximately equally between the two types of chloroplast. 4. After exposure of illuminated leaves to (14)CO(2) for 25sec., labelled malate, aspartate and 3-phosphoglycerate had similar fractionation patterns, and a large proportion of each was isolated with mesophyll chloroplasts. Labelled fructose phosphates and ribulose phosphates were mainly isolated in fractions containing parenchyma-sheath chloroplasts, and dihydroxyacetone phosphate had a fractionation pattern intermediate between those of C(4) dicarboxylic acids and sugar phosphates. 6. These results indicate that the mesophyll and parenchyma-sheath chloroplasts have a co-operative function in the operation of the C(4)-dicarboxylic acid pathway. Possible routes for the transfer of carbon from C(4) dicarboxylic acids to sugars are discussed.