Hyperfine structure from the four pyrrole nitrogens of heme. Hemin and hematin have been incorporated into single crystals of the condensed-ring planar hydrocarbon perylene, the approximate molecular ratio of heme to aromatic being 10(-3). X-ray diffraction and electron paramagnetic resonance (EPR) measurements indicate that these mixed crystals maintain their integrity when subjected to cryogenic temperatures and then returned to room temperature. EPR X-band orientation studies of single crystals at liquid helium temperature have revealed the following two important features: (1) presence of ligand hyperfine structure, which can be used to quantitate the distribution of unpaired metal electron on the ligands and (2) removal of the immediate fourfold symmetry of the ligand field about the metal, as evidenced by the rhombic character of the high-spin ferric system. The ligand hyperfine splittings have been interpreted as arising chiefly from an isotropic Fermi contact interaction. The observed splittings then indicate that 2.7 per cent of an unpaired spin is in each nitrogen 2s orbital. The g-values have been interpreted in terms of the spin-Hamiltonian: [Formula: see text] which gives g(x,y) = 6.00 +/- 24E/D and g(z) = 2.00 - 34(E/D)(2). For various hemin and hematin sites these formulae for the g-values are nicely obeyed when E/D is in the range 0.015 to 0.03.


The relationship of DNA replication to the control of protein synthesis in protoplasts of T4-infected Escherichia coli B. The relationship of DNA replication to the control of early enzyme synthesis and the formation of late proteins was studied in protoplasts of E. coli B cells infected with an amber mutant, T4am130. In this phage-host system the bacterial DNA is not completely degraded, and the onset and the extent of phage DNA synthesis could be regulated by use of 5-fluorodeoxyuridine and thymidine. It was found that DNA replication is necessary for the transcription of "late" regions of the phage genome and, thus, for the synthesis of late proteins. In addition, the sustained synthesis of late RNA and the tail-fiber protein was shown to require the continued replication of DNA. Furthermore, the mechanism regulating the cessation of early enzyme synthesis appeared to become operative shortly after the onset of DNA replication and required a limited amount of DNA synthesis for its full expression.


Translocation of mRNA codons. I. The preparation and characteristics of a homogeneous enzyme. This report describes a convenient scheme for the further purification of an E. coli enzyme which is required for the translocation step in protein biosynthesis. The homogeneous enzyme translocase appears to be a relatively large monomeric protein, having a molecular weight of approximately 72,000, and acts in a catalytic fashion during protein synthesis. It is also one of the major soluble macromolecular constituents of rapidly growing E. coli, comprising more than 2 per cent of the protein in ribosome-free extracts. Further, the rate of in vitro protein synthesis is linearly dependent upon the concentration of the pure enzyme until approximately one molecule of translocase is present per ribosome in reaction mixtures.


Virus-specific nucleic acids in SV40-exposed hamster embryo cell lines: correlation with S and T antigens. A number of homologous SV40-exposed hamster embryonic cell lines were examined for the presence of RNA complementary to SV40 DNA. Only those lines containing the SV40 T antigen were found to have such virus-specific RNA. In lines containing the SV40 S antigen, but not the SV40 T antigen, virus-specific RNA was not detected. These findings suggest that the S antigen is not coded for directly by the SV40 genome.


A fluorescence probe of energy-dependent structure changes in fragmented membranes. The reaction of the fluorochrome, 8-anilino-1-naphthalene-sulfonic acid (ANS), with fragmented membranes from beef heart mitochondria has been studied. ANS fluorescence is found to be enhanced 25-fold on binding to the membrane fragments in the absence of energy conservation, and this enhancement is increased to 35-fold in the membrane energized by substrate plux oxygen. The fluorescence of bound ANS depends upon the energy state of the membrane fragments, as indicated by the effects of ATP, substrates of the respiratory chain, oligomycin, and uncouplers. It is concluded that the changes of ANS fluorescence indicate structural changes of the mitochondrial membrane associated with energy conservation. The time course of energization is readily followed by ANS, and has a half-time of two seconds at 26 degrees .


A British abattoir survey of tumours in cattle, sheep and pigs.


Glucose-6-phosphatase activity in primary rat liver tumors induced by high doses of 4-dimethylaminoazo-benzene.


Histochemically observable alterations in enzyme pattern of rat myocardium, caused by parathion (E 605).


Viral and Mycoplasma pneumoniae infections in exacerbations of chronic lung disease.


An enzyme system of Mycobacterium tuberculosis that reacts specifically with isoniazid. I.


Studies of a biochemical lesion in experimental tuberculosis in mice. X. Mitochondrial swelling induced by cord factor in vivo and accompanying biochemical change.


Adaptation of the parainfluenza 3(EA--106) virus to a new host. I. Adaptation of the strain from tissue lines to the chick embryo.


Adaptation of the parainfluenza 3 (EA-106) virus to a new host. II. The EA-106 strain in cultures of L cells.


Avian reoviruses. I. Isolation and serological characterization.


Virus-like particles observed in plasma and white blood cells from birds with Marek's disease.


Avian reoviruses II. Physicochemical characterization and classification.


Conformational evidence for the non-identity of cytochromes  a and a3 in cytochrome oxidase.


Detection and characterization of the phosphorylated form of microsomal glucose-6-phosphatase.


Studies on in vitro RNA synthesis by chromatin fractions from rat liver and Novikoff hepatoma.


Organophosphate spin-label studies of inhibited esterases, alpha-chymotrypsin and cholinesterase.


Enzymatic pyrophosphorylation of 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine by cell-free extracts of Escherichia coli B.


A flavin-chlorophyll alpha complex resembling the P700 of photosynthesis.


The step sensitive to catabolite repression and its reversal by 3'-5' cyclic AMP during induced synthesis of beta-galactosidase in E. coli.


Ultrastructural features of functioning alpha- and beta-cell tumors.


Observations on the composition of certain colonic tumors.


Contiguous glioblastoma multiforme and fibrosarcoma with extracranial metastasis.


Nodular renal blastema. Definition and possible significance.


Simultaneous hypoglycemia and acute congestive heart failure.


Angiotensin and norepinephrine efflux.

Angiotensin and norepinephrine efflux.


Transformation of a mouse cell line by murine sarcoma virus (Moloney).


Effect of herpes simplex virus on human embryonic cells latently infected with reovirus type 3.


The stimulating effect of complete Freund's adjuvant on tumour induction by polyoma virus in mice and by Rous sarcoma virus in rats.


Carcinogenesis by avian sarcoma viruses. X. The decreased requirement for insulin-replaceable activity in serum for cell multiplication.


A study of the neutralization of rhinoviruses by means of a plaque-reduction test.


The immunologic response of the mouse to aerosols of an attenuated encephalomyocarditis virus strain.


The effect of carrageenan on delayed hypersensitivity reactions.


Release of gamma-aminobutyric acid into the fourth ventricle induced by stimulation of the cat's cerebellum.


Incorporation of 32P in vitro into triphosphoinositide and related lipids of rat superior cervical ganglia and vagus nerves.


P-nitrophenyl phosphatases of a membrane fraction from bovine cerebral cortex.


Effect of mitomycin C on thymidine-methyl-H3 utilization by Entamoeba histolytica propagated in CLG medium.


Carcinoma of cowper's gland: case report.


Cancer of the nasopharynx. Review of the literature. Report of 72 cases.


Reduction of transmitter release by D-tubocurarine.


Enzymatic release of phosphate from rat molar enamel by phosphoprotein phosphatase.


Avian pox in migrant and native birds in Panama.


Copper metabolism.

Copper metabolism.


Biosynthesis of vitamin A from beta-carotene.


Induction of brain tumors in newborn hamsters by simian adenovirus SA7.


Glutaminase activities and growth rates of rat hepatomas.


Kinetic, electrophoretic, and chromatographic studies on glucose-ATP phosphotransferases in rat hepatomas.


1-methyl-1-nitrosourea and dialkylnitrosamine depression of nicotinamide adenine dinucleotide.


Hematogenous metastasis of Yoshida ascites Hepatoma in the chick embryo liver: ultrastructural changes in tumor cells.


Effect of feeding amino azo dyes on mitochondrial swelling and contraction. Kinetic evidence for deletion of membrane regulatory sites.


Virus particles associated with the transplantable Novikoff hepatoma in the rat.


Sequential alterations in mitochondrial inner and outer membrane electron transport and in respiratory control during feeding of amino azo dyes; stability of phosphorylation. Correlation with swelling-contraction changes and tumorigenesis threshold.


The source of the nothing dehydrogenase activity in normal serum.


The use of tris buffer for u.v. spectrophotometric assay of GOT and GPT.


Viral and virus-like infections of the female genital tract.


Specific transplantation resistance against mouse tumour induced by Mouse Sarcoma Virus (Harvey).


Karyological analysis of mouse and rat embryo cells transformed by Murine Sarcoma Virus (Harvey).


Report of the panel for the symposium on immunity to selected equine infectious diseases.


Immunology of equine influenza.

Immunology of equine influenza.


On immunity to disease caused by equine herpesvirus 1.


The evaluation of an experimental bivalent equine influenza virus vaccine.


Comparative aspects of immunity against bovine and equine herpesviruses.


Comments on equine herpesvirus infection (equine rhinopneumonitis) and bovine herpesvirus infection (infectious bovine rhinotracheitis).


Biological requirements and control of equine rhinopneumonitis vaccine (live virus).


Comments on biologic requirements and control of equine rhinopneumonitis vaccine.


Attitudes of professional nonpsychiatric nurses toward mental illness.


The dilemma of the alcoholic patient in a psychiatric hospital.


Social problems involved in treating cancer of the larynx.


Enterovirus infections of the central nervous system.


Iatrogenic modification of tissue responses to infectious agents in the central nervous system.


Nursing in Greece.

Nursing in Greece.


Nursing action guide: hallucinations.

Nursing action guide: hallucinations.


Experimental strangulated intestinal obstruction.


Platelet adhesiveness in normal and bleeder swine as measured in a Celite system.


Glomerular (azotemic) osteodystrophy.

Glomerular (azotemic) osteodystrophy.


Psychiatric drugs and trends. Slang terms of drug abusers.


Studies on the subunit structure of rabbit liver fructose diphosphatase.


Separation and characterization of HeLa alkaline pyrophosphatase isoenzymes.


Psychology, extended care facility, and the community.


The effects of pyridoxal phosphate on rabbit liver and kidney fructose 1,6-diphosphatases.


Service occupation workers preference change and stereotype reduction toward their clients.


Creativity with retardation.

Creativity with retardation.


Phosphoserine phosphatase distribution in normal and neoplastic rat tissues.


Spin-labeled hapten studies of structure heterogeneity and cross-reactivity of the antibody active site.


The possible relationship between ultrastructure and biochemical state of heart mitochondria.


Geriatrics.

Geriatrics.


Transformation of mevalonic acid into isoprenoid compounds by enzymes from Pinus radiata. Activation of the overall reaction by phospholipids.


Hypoglycemic peripheral neuropathy.

Hypoglycemic peripheral neuropathy.


Peripheral pathways and properties of uterine afferents in the cat.


Nucleosidediphosphatase activity in plasma membrane of rat liver.


The bacterial metabolism of flavonoids: hydroxylation of taxifolin.


Countercurrent distribution of inosol lipids of plant seeds. The inositol lipids of plant seeds consist of phosphatidyl inositol, the phytoglycolipids, and a previously uncharacterized ceramide-phosphate-polysaccharide. These three species have been separated from each other and from the common glycerophosphatides by a series of simple countercurrent distributions, first as the naturally occurring Ca-Mg salts and subsequently in the Na salt form. The new ceramide-phosphate-polysaccharide is present in each of the four plant phosphatides examined (corn, soybean, flax, safflower). It is devoid of glucosamine but contains the other carbohydrate components commonly found in the phytoglycolipids. The basic structural unit of the new glycolipid consists of a ceramide-phosphate-inositol-hexuronic acid moiety to which the other sugars (galactose, mannose, arabinose) are attached. Flax ceramide-phosphate-polysaccharide has fucose in addition to the other sugars.


The mechanism of heparin stimulation of rat adipocyte lipoprotein lipase. Free fat cells and stromal-vascular cells were prepared from rat adipose tissue by incubation with collagenase. NH(4)OH-NH(4)Cl extracts of acetone-ether powders prepared from fat cells contained lipoprotein lipase activity but extracts of stromal-vascular cells did not. Intact fat cells released lipoprotein lipase activity into incubation medium, but intact stromal-vascular cells did not. The lipoprotein lipase activity of the medium was increased when fat cells were incubated with heparin, and this was accompanied by a corresponding decrease in the activity of subsequently prepared fat cell extracts. Heparin did not release lipoprotein lipase activity from stromal-vascular cells. The lipoprotein lipase activity of NH(4)OH-NH(4)Cl extracts of fat cell acetone powders is increased by the presence of heparin during the assay. This increase is not due to preservation of enzyme activity, but to increased binding of lipoprotein lipase to chylomicrons. Protamine sulfate and sodium chloride have little effect on the binding of lipoprotein lipase to chylomicrons, but they inhibit enzyme activity after binding to substrate has occurred. These inhibitors do, however, inhibit the stimulatory effect of heparin on enzyme-substrate binding.


Metabolism of cholesterol in the tissues and blood of the chick embryo. Three artificially inseminated laying White Leghorn hens were given 35-50 micro c of cholesterol-4-(14)C intravenously. Their subsequently produced eggs contained cholesterol-(14)C-labeled yolks. Some of the fertilized eggs were analyzed for cholesterol content and radioactivity. Other eggs were incubated until hatching. The specific activity of the cholesterol contained in the serum and tissues of newly hatched chicks was determined and compared with that of yolk sac, which was taken as representative of egg yolk cholesterol before its metabolic transfer into the chick embryo. The specific activities of cholesterol in intestine, liver, serum, heart, and skeletal muscle and the whole chick were 95-98% of that in yolk sac, but that of brain cholesterol was only 11% of this value. These results indicate that whereas most of the cholesterol in the chick originated from the egg yolk, cholesterol biosynthesis was active in the brain and provided about 90% of its cholestero content. Newly hatched chicks were found to be hyperlipemic compared with older chicks and had fatty livers with a high cholesterol content. Desmosterol was found in 9- and 15-day old chick embryos but not in the newly hatched chicks, in which the only sterol was cholesterol.


Long-chain bases in the sphingolipids of atherosclerotic human aorta. Long-chain bases were prepared from human aorta sphingomyelin by a combined enzymatic hydrolysis-alkaline hydrolysis procedure and these bases were isolated by thin-layer chromatography. Aldehydes, obtained from the long-chain bases by periodate oxidation, were converted to 1,3-dioxolane derivatives. Dioxolanes were identified and quantified by gas-liquid chromatography before and after catalytic hydrogenation, and before and after separation into saturated, monoene, and diene dioxolane fractions. The monoene dioxolanes were converted to aldehydes by reductive ozonolysis with dimethyl sulfide and these aldehydes were isolated and identified as dioxolane derivatives. The double bond positions in the major diene component were established by reductive ozonolysis and permanganate-periodate oxidation. Sphingenines in the cerebroside-sulfatide and sulfatide fractions of aorta were converted to aldehydes by the reductive ozonolysis of intact sphingolipids and these aldehydes were analyzed as the dioxolanes. Human aorta sphingomyelin contained significant amounts of 4-hexadecasphingenine, 4-heptadecasphingenine, sphinganine, 4-sphingenine, and 4,x14-sphingadienine. Small amounts of hexadecasphinganine, 4-tetradecasphingenine, a sphingadienine isomer, an unknown sphinganine, and two unknown diene long-chain bases were also found in sphingomyelin. The presence of a branched-chain 4-sphingenine was tentatively established and the possible presence of a sphingenine isomer was suggested. The major sphingenines were the same in the sphingomyelin, sulfatide, and cerebroside-sulfatide fractions of human aorta.


Separation of neutral lipids of shark liver by "dry-column" chromatography. "Dry-column" chromatography in mixed solvents has been successfully used to separate gram quantities of neutral lipids from shark liver oil into simpler fractions.


Dry-column chromatographic isolation of fatty acid esters of phorbol from croton oil. The family of mixed fatty acid esters of 4,9,12,13,20-pentahydroxy-tiglia-1,6-dien-3-one (phorbol) has been isolated from croton oil by column chromatography on silica gel and preparative dry-column chromatography on silica gel HF/254 packed in a cellophane membrane. The tumorpromoting agents are obtained rapidly and with minimum difficulty.


Attempts to cultivate the equine infectious anemia virus in various types of cells.


Arrhenoblastoma: case report.

Arrhenoblastoma: case report.


Free radicals formed in aliphatic polyamino acids by exposure to hydrogen atoms.


Microangiopathic haemolytianaemia.

Microangiopathic haemolytianaemia.


The influence of the renin-angiotensin system on renal function: possible role in advanced liver disease.


Conjugation of steroid hormones by breast-cancer tissue and selection of patients for adrenalectomy.


Site of action of venom of Microbracon hebetor Say (Braconidae, Hymenoptera).


Survival in childhood malignancies: assessment of the influence of age, sex, and tumor type, with emphasis on "long-term survivors".


Relation of virus-induce cell fusion and chromosome pulverization to mitotic events.


Skeletal and reticular tissue disorders produced in mice by agent(s) from Sarcoma 37.


Rates of albumin and transferrin synthesis in vitro in rat hepatoma-derived H4II-EC cells.


Biochemical, pathological, and genetic aspects of a spontaneous mouse hepatoma.


Report on 141 childhood malignancies observed in Jamaica.


Growth and nucleic acid synthesis in normal cells and cells infected with Rous sarcoma virus.


Influence of hormones on N-2-fluorenyldiacetamide-induced hyperplastic hepatic nodules in rats.


Reproductive capacity and viability at higher temperatures of various transformed hamster cell lines.


Canine papilloma: the structural characterization of oral papilloma virus.


"Rescued" SV40: increased transforming efficiency in mouse and human cells. Plaque-size mutants of simian virus 40 (SV40) have been characterized with respect to their ability to transform mouse and human cells in vitro. Large plaque mutants of SV40 were extremely inefficient at transforming mouse or human cells, requiring approximately 50 times more infectious particles per transformation event than did the small plaque mutants. The minute plaque SV40 was the most efficient, requiring less than 10(3) plaque-forming units per transformation in mouse Balb/3T3 cells and only 1.5 x 10(4) plaque-forming units per transformation unit in the "susceptible" human strains. Both small and minute plaque virus were unable to help the large plaque virus to transform. Virus "rescued" from transformed 3T3 cells by cocultivation with monkey kidney cells was more efficient at transformation than the parental type. The increased efficiency could be shown for each plaque type and in human as well as in mouse cells. The property of "high efficiency transformation" is stable through at least two passages in monkey kidney cells.


Spin-labeled transfer RNA.

Spin-labeled transfer RNA. The alpha-amino group of valyl-tRNA has been combined chemically with a nitroxide spin label through an amide linkage. This spin-labeled aminoacyl-tRNA has an electron paramagnetic resonance spectrum which reflects the mobility of the aminoacyl end of the tRNA molecule. The rate of tumbling of the spin label has been measured as a function of temperature and ionic strength. An abrupt transition in the nature of the motion occurs at a temperature which is sensitive to the ionic strength of the medium. This change in behavior of the aminoacylated spin label occurs at the same temperature as the midpoint of the optical density melting curves in dilute salt solutions. However, when denaturing agents such as dimethylsulfoxide or urea are added to the solution, the spin melting temperature is lower than the melting temperature as measured by the change in optical density. This suggests that under these conditions the local region of the aminoacyl end of the molecule denatures before the rest of the molecule is disrupted.


Surface-specific characteristics of a contact-inhibited cell line containing the SV40 viral genome. A cytoagglutinin purified from wheat germ lipase agglutinated five related murine fibroblast cell lines in the order of their saturation densities. One cell line, which was transformed by the oncogenic papova virus SV40 but which had a low saturation density, agglutinated poorly. Preincubation of cells with trypsin increased their agglutinability, but trypsin made no cell line more agglutinable than the cell line with the highest saturation density.


An ESR study of the reaction of OH. free radical with cysteine and related sulfhydryl compounds. UCLA-12-686.


The role of individual spleen cells in the interferon response of the intact mouse. A method is described for the detection of interferon production by individual spleen cells of mice after intravenous virus infection. Although mouse spleen plays a major role in the total interferon response to Newcastle disease virus, the number of spleen cells participating is a small fraction of the total, suggesting that interferon formation is to some extent a specialized cell function. Monolayers of mouse embryo cells, after brief contact with agar-suspended spleen cells from interferon-producing mice, have roughly circular foci of cells remaining after removal of agar and vesicular stomatitis virus challenge. The numbers of such foci correlate directly with the number of spleen cells, concentration of inducing virus in the inoculum, duration of contact of cells with mouse-embryo indicator monolayers, time after interferon induction, and serum interferon titer. With this technique, evolution of the interferon-forming cell response in spleens of virus-infected mice has been studied.


Correction of malignant hypertension and returen of renal function following late renal artery embolectomy.


Studies of the renin-angiotensin-aldosterone system in patients with hypertension and in normal subjects.


Propagation of MM virus in L cells.

Propagation of MM virus in L cells. MM virus (mouse-brain stock) replicated to a limited extent in L cells without cytopathic effects; the average yield was less than 1 plaque-forming unit/cell. Passage in BHK-21 cells resulted in MM virus [MM(BHK)] which replicated to high titers (200 to 300 plaque-forming units/cell) in L cells with complete cytopathic effects. Appearance of mature MM(BHK) virus in L-cell cultures begins 4 hr after infection and is completed by 8 hr. Release of mature virus was slow (less than 1% at 8 hr) but was completed by 24 hr.


Procedure for the evaluation of the virucidal effectiveness of an ethylene oxide gas sterilizer. A quantitative, reproducible method was developed for the evaluation of the virucidal activity of test gases. Using this method, we determined the virucidal effectiveness of a Steri-Vac ethylene oxide gas sterilizer. Wool gabardine material was exposed to high concentrations of herpes simplex, vaccinia, parainfluenza, or polio viruses and was processed through the sterilizer. Two time-temperature cycles of the machine, 29 C for 180 min and 60 C for 48 min, were used in separate experiments. The viruses were exposed to the gas when freshly pipetted onto the fabric or when pipetted on the material and allowed to dry 16 to 24 hr. In two experiments carried out under each condition, the virus titers were reduced by the sterilization process to less than detectable limits. These titer reductions were for the herpes virus >/= 2.7 to 5.0 log, for vaccinia virus >/= 4.0 to 6.1 log, for parainfluenza virus >/= 1.8 to 4.9 log, and for poliovirus >/= 4.9 to 7.7 log. The observed reductions in virus titers were the same whether the virus-contaminated fabrics were sealed in polyethylene packages or held in open petri dishes during exposure to ethylene oxide.


Propagation of MM virus in continuous cell lines. Baby hamster kidney (BHK), McCoy, and L cell lines were found to be suitable for isolation of MM virus from infected mouse brain tissue. The virus was recovered in high titer in the first passage in BHK and McCoy cells, with concomitant cytopathic effect (CPE). In L cells, virus yield was lower than in the other two cell lines and CPE was incomplete. Adaptation of the virus to BHK and McCoy cells by serial passages was evidenced by accelerated development of the CPE and increase in the virus titer. Plaques were obtained in all three cell lines when inoculated with infected mouse brain or with the tissue culture-propagated virus. In the BHK cells, the virus release preceded the appearance of CPE and maximal yield of virus was obtained after 1 to 3 days of incubation, depending on the size of inoculum. The BHK-propagated virus had the same lethality for mice as did the mouse brain-propagated stock, and there was no difference in the course of the disease caused by the two preparations.


Biochemical and clinical characteristics and antibiotic susceptibility of atypical Enterobacter cloacae. The characteristics of an atypical group of the family Enterobacteriaceae resembling Enterobacter cloacae were studied. The urinary tract was the most common source of these organisms, and most strains represented infections of secondary clinical significance. In contrast to typical Enterobacter strains, the atypical strains were highly susceptible to the cephalosporins; otherwise, there was a high degree of susceptibility to five other antibiotics and resistance to ampicillin except in very high concentration.


Yellow fever vaccine. II. Antigenicity and neurovirulence of a vaccine seed free from avian leukosis virus. Avian leukosis virus (ALV)-free candidate primary and secondary seed lots were indistinguishable from corresponding ALV-contaminated lots with respect to (i) potency as measured by titration in newborn and weanling mice and in the MA-104 plaque system, (ii) degree of viscerotropism as measured by viremia in monkeys, (iii) neurotropism as determined by the monkey neurovirulence test, and (iv) potency as determined by antibody response in monkeys inoculated by the intracerebral route.


Dapsone and peripheral motor neuropathy.


Granular cell myoblastoma.

Granular cell myoblastoma.


The incidence of neoplastic trophoblastic disease in Australia.


A structural basis for the transphosphorylation of nucleotides with hydroxyapatite.


Carcinogen testing in the new-born mouse.


Sensitivity of newborn mice to carcinogenic agents.


Nitrosoamines: a jig-saw puzzle with missing pieces.


Diabetes mellitus initially presenting as peripheral neuropathy in the upper limb.


Effect of temperature on the formation of radicals by hydrogen-atom bombardment.


Cyclic 3',5'-nucleotide phosphodiesterase in brain.


The reducing action of elastic tissue in ferric ferricyanide. A histochemical analysis.


The role of histochemical and biochemical preparation methods for the detection of glycogen.


Patterns of infections with adenovirus types 4, 7 and 21 in military recruits during a 9-year survey.


Granular cell myoblastoma of the esophagus. A case report.


Adrenal catecholamine and cortisol secretion during extracorporeal circulation in dogs.


Immunofluorescence in the study of Marek's disease. I. Detection of antigen in cell culture and an antigenic comparison of eight isolates. The indirect fluorescent-antibody (FA) test was applied to the detection of Marek's disease (MD) antigen in cell culture and antibody in the serum of birds. For the detection of antigen, sera were obtained from birds hyperimmunized with the JM strain of MD. MD antigen could be detected in the nucleus and in the cytoplasm of duck and chick embryo fibroblasts and in those of chick kidney cells infected with material known to contain the MD virus. Uninoculated cultures of chicken cells were always free of MD antigen. When chick kidney cells were infected with a stock cellular preparation of MD virus, infected cells could be detected after 24 hr with the FA test. At this time no cytopathological areas were seen by conventional light microscopy. By 7 days after infection, the same number of infected areas were detected by both methods, and the fluorescent areas coincided with the cytopathological areas. This indicates that the fluorescent areas and the areas with cytopathology are caused by the same agent. A straight-line relationship between the dilution of inoculum and the number of fluorescent or morphological foci obtained indicates that one infectious unit produced one fluorescent or morphological focus. In addition, this time sequence study confirmed the cell association of the virus and demonstrated the cell-to-cell spread of infection. Cell cultures inoculated with eight different isolates of MD were tested in all combinations with sera prepared against the same isolates. The antigens were indistinguishable from one another, indicating that either the strains are antigenically identical or there is a common antigen or contaminant in all of them so that they stained equally well. The FA test can detect MD antigen before cytopathological areas develop in cell culture; however, the small size of the area usually examined precludes its use in initial isolations in which only a small number of infectious units are present in the inoculum. MD-infected cells contain a heat-stable antigen similar to that found in herpes simplex-infected cells.


Protein composition of the structural components of vesicular stomatitis virus. Digitonin, a sterol glycoside which complexes with cholesterol, stripped off the envelope of vesicular stomatitis (VS) virions and liberated two viral structural proteins, 83% of P6 and 53% of P4. Deoxycholate also disrupted VS virions but released nucleocapsid cores which could be identified by higher buoyant density, ratio of incorporated (3)H-uridine to (14)C-protein, and electron microscopy. The major nucleocapsid protein was P5 but varying amounts of the minor protein aggregate P2 were present, depending on the concentration of urea used for extraction. P2 appeared to be a polymer of P5. Two other minor structural proteins, P1 and P3, could not be located in the virion. From these data, we conclude that the three microscopically identifiable structures of VS virions are each composed primarily of a single major protein, as follows: P6 = envelope protein, P4 = protein of underlying "shell," and P5 = nucleocapsid protein.


Fusion of vesicular stomatitis virus with the cytoplasmic membrane of L cells. At an early stage in infection, vesicular stomatitis viruses were attached to the surface of L cells by fusion of the viral and cell membranes.


Effect of ultraviolet irradiation and actinomycin D on polyoma virus replication in mouse embryo cell cultures. Ultraviolet irradiation and actinomycin D impair the capacity of mouse embryo (ME) cells to support the replication of polyoma virus, but not of encephalomyocarditis (EMC) virus. The loss in capacity for polyoma virus synthesis was an "all-or-none" effect and followed closely upon the loss in cellular capacity for clone formation. Cells treated with either agent produced polyoma "T" antigen, but did not synthesize polyoma structural protein. Infection of untreated ME cells with polyoma virus produced marked stimulation of both deoxyribonucleic acid (DNA) synthesis and ribonucleic acid (RNA) synthesis. ME cell cultures irradiated with ultraviolet for 30 sec at 60 muw/cm(2) or treated with actinomycin D at 0.1 mug/ml for 6 hr prior to infection were incapable of synthesizing DNA or RNA, even after infection with polyoma virus. Irradiation of cells during infection produced cessation of synthesis of both RNA and DNA. Addition of actinomycin D during infection did not inhibit DNA synthesis but abolished RNA synthesis and reduced the yield of polyoma virus to 10% of that in untreated infected cultures. Both agents lost the ability to prevent replication of a full yield of polyoma virus when administered 30 hr after infection or later. The period after which neither agent inhibited polyoma replication corresponded with the period at which maximal RNA synthesis in untreated infected cultures had subsided. It can be concluded on the basis of the data presented that the functional integrity of the mouse embryo cell genome is required for the replication of polyoma virus, but not for EMC virus. Whereas the requirement for cellular DNA-dependent RNA synthesis for polyoma virus replication has been demonstrated, the exact nature of the host-cell function remains to be elucidated.


Double-blind, cross-over study comparing chlordiazepoxide, conjugated estrogens, combined chlordiazepoxide and conjugated estrogens, and placebo in treatment of the menopause.


Encephalomyocarditis virus ribonucleic acid polymerase associated with 150S cytoplasmic particles. Cytoplasmic particles which sedimented at 150S were the smallest structures containing detectable viral ribonucleic acid polymerase in mouse cells infected with encephalomyocarditis virus.


Effect of endocrines on central nervous system. I.


Role of psychotropic therapy in estrogen-deficient menopausal patients.


Acetylcholine action: biochemical aspects.


Quantal secretion from adrenal medulla: all-or-none release of storage vesicle content. Neurogenic secretion of catecholamines from the adrenal medulla in rabbits, induced by administration of insulin, caused decreases in both the dopamine-beta-hydroxylase activity and the catecholamine content of the storage vesicle fraction. After sedimentation through a sucrose density gradient, the storage vesicles obtained from insulin-treated animals had the same density and the same ratio of dopamine-beta-hydroxylase to catecholamine as did vesicles from untreated animals. These and other data indicate that neurogenic secretion from the adrenal medulla occurs by an all-or-none release from the storage vesicles.


Juvenile angiofibroma.

Juvenile angiofibroma.


Granular cell myoblastoma. Clinicopathologic study of forty-two patients.


Vesicular stomatitis virus infections in Panamanian primates and other vertebrates.


Arousal and nonarousal enuretic events.

Arousal and nonarousal enuretic events.


Recent studies othe possible role of viruses in human cancer.


Metabolism and function studied in single neurones.


Cytomegalovirus hepatitis in an adult. An autopsy report.


Biosynthesis of chlorophylls.

Biosynthesis of chlorophylls.


Relation between the concentration of ATP and that of NADH and excess porphyrin formation in the liver of rats during experimental porphyria.


Congenital cytomegalic inclusion disease following intrauterine transfusion. Congenital cytomegalic inclusion disease occurring in two infants who received intrauterine transfusions for severe haemolytic disease due to rhesus isoimmunization may have been transmitted in the donor blood.


Relapsing amoebic colitis of 12 year's standing exacerbated by corticosteroids.


Electron spin resonance study of the free radicals of 3-methylcholanthrene oxi-derivatives.


Effects of trypsin on replication of parainfluenza-3 virus in HEp-2 cell cultures.


Aldehyde dehydrogenase in pseudomonads.

Aldehyde dehydrogenase in pseudomonads.


Practically applicable results of twenty years of research in endocrinology.


Evidence for a methionyl residue in the active site of the cytoplasmic malate dehydrogenase from pig heart.


Phosphatase activity of coagulase-positive and coagulase-negative staphylococci and their correlation with pathogenicity.


Transplacental transmission of polio antibodies--flocculating and neutralizing.


Evaluation in clinical practice of the fluorescent amoebic antibody test. Serum fluorescent antibody levels against Entamoeba histolytica were determined in patients with amoebic infection and in control cases. Titres of 1: 64 or above were obtained only in cases of active or recent amoebic infection, and were recorded in 75.0% of intestinal cases and in 95.2% of extraintestinal cases. Titres given by symptomless carriers of Entamoeba histolytica, though not exceeding 1: 32, were proportionately higher than those obtained in normal subjects or in patients with non-amoebic disorders.


A case of fatal Clostridium welchii toxaemia due to ward infection.


Incubation of cerebral microsomes with [3H] acetyl phosphate.


A technique for functional liver autotransplantation. Observations on survival, function, and regeneration of the autotransplant.


Wilms' tumor. A current plan of treatment.


Radiotherapy of Wilms' tumor and neuroblastoma.


Herpes encephalitis. I. The clinical picture.


Herpes encephalitis. II. Pathology of herpes encephalitis.


Naturally occurring carcinogens in foods.


Mode of action of DDT and allethrin on nerve: cellular and molecular mechanisms.


The metabolic and bactericidal defect in chronic granulomatous disease.


Comparative chronic inhalation toxicity of beryllium ores, bertrandite and beryl, with production of pulmonary tumors by beryl.


The search for human hepatitis virus.

The search for human hepatitis virus.


A critical review of methodology of sleep learning experiments.


Monoamines and the EEG stages of sleep.

Monoamines and the EEG stages of sleep.


"Pickwickian syndrome" and the influence of weight reduction on the clinical state and polygraphic picture.


The influence of centrophenoxine (Lucidril) on the course of sleep deprivtion in alcoholics.


Nocturnal sleep of hypersomniacs.

Nocturnal sleep of hypersomniacs.


The sleep cycle duration in normal and epileptic cats and humans.


A hand-operated, ultrasound scan system for ophthalmic evaluation.


Experimental design in clinical drug research: some practical implications.


Gram negative infections in burns and their chemotherapy.


Treatment of ophthalmic hemorrhage by premarin.


How are insect circadian rhythms controlled?


Cyclic AMP regulates catabolite and transient repression in E. coli.


Inhibitory effect of interferon on murine sarcoma and leukaemia virus infection in vitro.


Chromosome changes in human cells induced by herpes simplex, types 1 and 2.


Effect of barbiturates on dream content.


Granular-cell myoblastoma of the hard palate.


Respiratory changes during seed germination. Histological distribution of respiratory enzymes and mobilization of fat reserves in castor bean endosperm and peanut cotyledons. Germinating peanut cotvledons and germinating castor bean endosperm have been compared with respect to their rates of fat dissimilation and with respect to the anatomical distribution of respiratory activity. The lipid mobilization is much slower in peanut cotyledons than in castor bean endosperm. Light has essentially no effect on either system. As germination progresses, the majority of the succinic dehydrogenase and cytochrome oxidase activities become localized in the vein regions of peanut cotyledons. In the castor bean endosperm these two activities are uniformly distributed throughout the storage parenchyma and increase with germination until the organ becomes soft and visibly senescent.


Neoplastic transformation of hamster astrocytes in vitro by simian virus 40 and polyoma virus. Astrocytes in cultures of brain cells from fetal or newborn hamsters undergo neoplastic transformation after infection with simian virus 40 or polyoma virus. Subcutaneous or intracerebral inoculation of the transformed brain cells into newborn or adult hamsters produces progressively enlarging astrocytomas at the sites of injection. Astrocytomas produced by polyomatransformed cell lines are histologically better differentiated, but grow more rapidly and metastasize more frequently, than astrocytomas produced by cell lines transformed by simian virus 40. These observations make available in vitro models of virus-induced oncogenesis in astrocytes and provide simple techniques for obtaining astrocytoma cell lines suitable for screening studies of chemical agents effective against astrocytomas.


Human granulocyte collagenase.

Human granulocyte collagenase. A collagenase, operative at neutral and alkaline pH, has been extracted from the granule fraction of human granulocytic leukocytes. It digests reconstituted collagen fibrils and reduces the viscosity of collagen solutions. Cleavage of collagen in solution with purified enzyme produces the discrete products characteristic of other animal collagenases.


The regulation of ACTH secretion.

The regulation of ACTH secretion.


Production of circulating interferon in the bovine species.


Serologic comparisons of infectious canine hepatitis and toronto A26-61 canine adenoviruses.


Interferon induction in the bovine species by infectious bovine rhinotracheitis virus.


Characteristics of in vitro infection of chicken kidney cell cultures with a herpesvirus from Marek's disease.


Susceptibility of cultured mammalian cells to infection with a herpesvirus from Marek's disease and T-virus from reticuloendotheliosis of chickens.


Effect of polysorbate 80 and ether on hemagglutination titers of certain myxoviruses.


Pyrophosphate as a selective inhibitor of macromolecule synthesis in normal and leukemic leukocytes.


2,4-dinitro-5-ethyleneiminobenzamide (CB 1954): a potent and selective inhibitor of the growth of the Walker carcinoma 256.


Kinetic studies of the mechanism of S-adenosylmethionine synthetase from yeast.


The action of staphylococcal nuclease on synthetic substrates.


Analogs of inosine 5'-phosphate with phosphorus-nitrogen and phosphorus-sulfur bonds. Binding and kinetic studies with inosine 5'-phosphate dehydrogenase.


Effect of quercetin on membrane-linked activities.


Metabolic control and structure of glycolytic enzymes. 8. Reversal of the dissociation of rabbit muscle pyruvate kinase into unfolded subunits.


The stoichiometry and site specificity of the uncoupling of mitochondrial oxidative phosphorylation by salicylanilide derivatives.


Comparative studies on the carbohydrate-containing membrane components of normal and virus-transformed mouse fibroblasts. I. Glucosamine-labeling patterns in 3T3, spontaneously transformed 3T3, and SV-40-transformed 3T3 cells.


Comparative studies on the carbohydrate-containing membrane components of normal and virus-transformed mouse fibroblasts. II. Separation of glycoproteins and glycopeptides by sephadex chromatography.


Spin-labeled hemoglobin derivatives in solution, polycrystalline suspensions, and single crystals.


Catalytic mechanism of pig heart mitochondrial malate dehydrogenase studied by kinetics at equilibrium.


A mannose- and erythritol-containing glycolipid from Ustilago maydis.


Isolation of rat liver mitochondrial membrane fractions and localization of the phospholipase A.


Chemistry and metabolism of sphingolipids. Chemical synthesis of 2-amino-1-hydroxyoctadecan-3-one (3-ketosphinganine).


Inhibition of glutamine synthetase by methionine sulfoximine. Studies on methionine sulfoximine phosphate.


The mechanism of action of ethanolamine deaminase. V. The photolysis of enzyme-bound alkylcobalamins.


Effect of chemical carcinogens on virus-induced Rous sarcoma.


Ribonucleotide reductase activity in cell-free extracts of Yaba poxvirus tumor and normal monkey tissues.


The oncogenic effects of nontransforming viruses from avian myeloblastosis virus.


Precipitin response of cattle to bovine papilloma virus.


Hexokinase isozyme patterns of experimental hepatomas of rats.


Excitability changes in interneurons of an epileptic focus during the development of tonic seizures.


The presence of inhibitory nerves in the colon after sympathetic denervation.


Acetylcholine release from motor-nerve endings in rats treated with neostigmine.


Studies on DNA methylase activity in mammalian tissue.


Cytochrome oxidase activity in different hypothalamic areas. Influence of castration.


Analysis of the convulsant action of pentylenetetrazol.


IgG as the main immobilization factor in rabbit antiserum against Entamoeba.


Effects of chronic melatonin and saline injections on pituitary adrenal secretion.


The duration of action of the synthetic pentacosapeptide D-serine-1-norleucine-4-valinamide-25-beta-1-25-corticotrophin (DW-75) in man.


Synthesis of NAD and catabolism of nicotinic acid in the isolated perfused liver of normal and X-irradiated rat.


Disposition of neurotransmitters in experimental mouse glioma.


Electron transport system of the protoheme-requiring anaerobe Bacteroides melaninogenicus. Protoheme is essential for the growth of some strains of Bacteroides melaninogenicus. At low concentrations in the growth medium, protoheme determines the doubling time, total cell yield, and amount of cytochrome per bacterium. At high protoheme concentrations, the doubling time, total cell yield, and amount of enzymatically reducible cytochrome appear to remain nearly constant, and protoheme is accumulated by the cell. The accumulated protoheme can support the growth of the bacterium for at least eight generations in a protoheme-free medium. When growth and cytochrome content are proportional during growth at low protoheme concentrations, the bacteria incorporate 10 to 20% of the total available protoheme into a membrane-bound respiratory system. This respiratory system includes cytochrome c, a carbon monoxide-binding pigment, and possibly flavoproteins. The pigments can be reversibly reduced by reduced nicotinamide adenine dinucleotide or endogenous metabolism and can be oxidized anaerobically by fumarate or by shaking in air. Electron transport is inhibited by 2-n-nonyl-4-hydroxy-quinoline-N-oxide.


Mitochondrial alterations in the spinal ganglion cells of the rat accompanying the long-lasting sensory disturbance induced by capsaicin.


Enzymatic degradation of diazinon by rat liver microsomes.


Some current problems in infections of the skin with the virus of herpes simplex and varicella-zoster.


Corticosteroidogenic effect of long-acting beta 1-24corticotrophin (CIBA 42.915 BA).


Intestinal Clostridium perfringens in rheumatoid arthritis and other collagen diseases.


Possible role of amygdala in regulation of gastric secretion in chronic fistula rats.


Differential regulation of blood flow to red and white muscle in sleep and defense behavior.


Electrophysiological correlates of visual perceptual masking in monkeys.


The rubrospinal tract. II. Facilitation of interneuronal transmission in reflex paths to motoneurones.


Control of cell multiplication in uninfected chicken cells and chicken cells converted by avian sarcoma viruses.


T-antigen and DNA synthesis in macrophages infected with polyoma virus.


Immunoreactive fragments of angiotensin II in blood.


Studies on mouse leukemia viruses. I. Isolation and characterization of a group-specific antigen.


Enhancement and inhibition of avian sarcoma viruses by polycations and polyanions.


The structure of the respiratory syncytial virus.


Respiratory viruses. Report of a WHO scientific group.


Cytology of carcinoma of the trachea.

Cytology of carcinoma of the trachea.


Studies on hydrolytic enzyme activity in the connective tissue of the human periodontal ligament. Observations apart from areas of inflammation.


Carcinoma in pleomorphic adenoma of the parotid gland.


A clinical and metabolic study of masculinizing arrhenoblastoma.


Brenner's tumor with theca cell luteinization and hormonal function.


Charge-transfer complexes in biological systems.


Free radicals from biological purines and pyrimidines.


Electronic structure of iron in porphyrin complexes.


Electron-nuclear and electron-electron spin interactions in the study of enzyme structure and function.


Tyrosine fluorescence in proteins.

Tyrosine fluorescence in proteins.


Electronic structures and spectra of flavins: an improved Pariser-Parr-Pople Mo and semiempirical unrestricted Hartree-Fock computations.


A proposed mechanism for subunit interaction in hemoglobin.


Enzyme envelopes on colloidal particles.


In vitro production of 25-hydroxycholecalciferol.


Induction of respiratory control by K+ in mitochondria.


A cyclic AMP--stimulated protein kinase in adipose tissue.


Cytological evidence for the long "quiescent" stage of gastric cancer in 2 patients with pernicious anemia.


Antibody pattern against polioviruses in healthy adult population of Lucknow (India).


Tarsal tunnel syndrome.

Tarsal tunnel syndrome.


Tumor board proceedings.

Tumor board proceedings.


Tricholemmoma.

Tricholemmoma.


Application of magnesium silicate adsorption chromatography to the determination of mephobarbital and diphenylhydantoin in tablets.


Incorporation of 14C-tryptophan into 14C-protein by cultured rat pineals: stimulation by l-norepinephrine. Organ cultures of individual rat pineals incorporate (14)C-tryptophan into proteins at a nearly constant rate for at least 48 hours. Previous studies have shown that these cultures also convert (14)C-tryptophan to serotonin, melatonin, and 5-hydroxyindoleacetic acid, and release these indoles into the media. The formation of (14)C-protein from (14)C-tryptophan is accelerated by the addition to the culture medium of l-norepinephrine or related catecholamines but is not modified by serotonin, melatonin, or 5-hydroxyindoleacetic acid. One mechanism by which norepinephrine stimulates the synthesis of (14)C-protein from (14)C-tryptophan involves increasing the uptake of the (14)C-tryptophan into pineal parenchymal cells, inasmuch as (1) norepinephrine increases the intracellular content of (14)C-tryptophan as well as its conversion to its major products, (14)C-protein and (14)C-indoles; (2) norepinephrine does not stimulate (14)C-protein synthesis in pineal organs which contain a previously fixed amount of (14)C-tryptophan; and (3) norepinephrine does not stimulate (14)C-protein synthesis from (14)C-methionine or (14)C-leucine. The finding that norepinephrine, but not serotonin, can stimulate the incorporation of (14)C-tryptophan into pineal proteins is consistent with the hypothesis that norepinephrine is the neurotransmitter substance utilized by pineal sympathetic nerve endings.


The use of an ethidium analogue in the dye-buoyant density procedure for the isolation of closed circular DNA: the variation of the superhelix density of mitochondrial DNA. The separation between open and closed circular DNA in buoyant CsCl gradients containing intercalating dyes depends on the superhelix density of the closed form. These separations are about 1.8 times larger with propidium iodide than with ethidium bromide. The superhelix densities of mitochondrial DNA from HeLa cells and Lytechinus pictus eggs appear to be about two thirds that of mitochondrial DNA from rat and rabbit liver.


The isolation of two enzyme-ribonucleic acid complexes involved in the synthesis of foot-and-mouth disease virus ribonucleic acid. The foot-and-mouth disease virus-RNA polymerase complex was released from membrane particulates present in the cytoplasm of infected baby hamster kidney cells. The soluble polymerase complex was fractionated by zonal centrifugation in sucrose gradients. Two polymerase complexes (RNA and protein complex) active in the cell-free system were isolated and had S-rate ranges of 20-70S and 100-300S, respectively. The light polymerase complex contained 20S double-stranded RNA; and the heavy polymerase complex contained a polydisperse, partially RNase-resistant RNA. The cell-free product of these two polymerase complexes was analyzed by zonal centrifugation in sucrose gradients. The light polymerase complex synthesized only 20S double-stranded RNA. The product of the heavy polymerase complex contained no detectable 20S double-stranded RNA and only a peak of single-stranded RNA with S-rate corresponding to 37S viral RNA. A third polymerase complex was isolated with S-rate greater than 300S, and it contained a polydisperse, partially RNase-resistant RNA. This third polymerase complex synthesized both 37S viral RNA and 20S double-stranded RNA in the cell-free system, and it is probably the native polymerase complex still bound to cellular particulates.


A quantitative comparison of formation of spontaneous and virus-produced viable hybrids. Ultraviolet-inactivated Sendai virus used in conjunction with a selective system reproducibly gives high yields of viable hybrid cell lines. With two different crosses, the frequency of hybrid colonies obtained after virus treatment was found to be 100 times greater than the frequency of spontaneous hybrids, and this increase was found to show little variation between 33 and 1000 hemagglutinating units of virus. No differences have been found between the properties of hybrids obtained after Sendai treatment and those obtained from untreated mixed cultures of parental cells.


Cytochrome oxidase deficiency in Wilson's disease: a suggested ceruloplasmin function. The hypothesis is advanced that ceruloplasmin functions in enzymatic transfer of copper to copper-containing enzymes, such as cytochrome oxidase. To test this hypothesis, leucocytes from Wilson's disease patients, heterozygous carriers, and normal subjects were assayed for cytochrome-oxidase activity. The data reported here show markedly reduced levels of activity in Wilson's disease cases and moderate reductions in heterozygous individuals relative to normal controls. These observations and a close correlation between the level of cytochrome-oxidase activity in the leucocytes and ceruloplasmin in the serum tend to support the hypothesis.


Differences in the transfer RNA's of normal liver and Novikoff hepatoma. -A comparison of the elution profiles of 18 aminoacyl-tRNA's from Novikoff hepatoma with those from normal liver on a methylated albuminkieselguhr column revealed the occurrence of new species of tRNA for histidine, tyrosine, and asparagine in the hepatoma. In addition, the hepatoma tRNA's for arginine, isoleucine, lysine, methionine, serine, alanine, and tryptophan eluted at a higher salt concentration than the corresponding tRNA's of normal liver. The remaining eight amino acids did not show any significant differences in the elution profiles.


A difference in the architecture of the surface membrane of normal and virally transformed cells. Several tissue culture cell lines that were transformed by a tumor virus have been found to react with an agglutinin, while under identical conditions their untransformed parent cell lines did not agglutinate. Since a short treatment of the parent cell line with low concentrations of proteases exposed the same agglutinin receptor sites in a fashion indistinguishable from the transformed cells, it is proposed that both viral and chemical transformation produce changes in the architecture of the membrane, identical to those of the proteases.


Scintillation camera scanning of the salivary glands.


Diet and dental caries. The phosphate sequestration hypothesis.


Histochemistry of the neuromuscular spindles in the chicken during development.


Control of cholesterol synthesis in hepatomas: the effect of bile salts.


Enzymatic synthesis of lycopene from [4-14C]isopentenyl pyrophosphate.


Occurrence and nature of O-alkyl and O-alk-I-enyl moieties of glycerol in lipids of Morris transplanted hepatomas and normal rat liver.


Hydrolysis of steroid phosphates by mouse tissue phosphatases.


X-amidinotransferase in viomycin biosynthesis.


Serological properties of bacterial fractions of the Clostridium perfringens type A strains causing acute food poisonings.


A possible role for cyclic AMP in gibberellic acid triggered release of alpha-amylase in barley endosperm slices.


Potentiation of cutaneous inhibition by alcohol.


Phosphatases of "Tetrahymena pyriformis".


The physiological maturity of adolescent psychiatric patients, juvenile delinquents and normal teenagers.


Some implications of the sleep patterns of pregnancy for postpartum emotional disturbances.


In vitro and in vivo virostatic properties of alkylated pyrimidines against DNA and RNA viruses.


A preliminary note on a recent increase in the incidence of hepatoma in the West Nile District of Uganda.


Acute effects of various gonadotropins and other pituitary hormones on preovulatory ovarian progestin secretion in hypophysectomized rats.


Dianemycin, an antibiotic of the group affecting ion transport.


Metabolism of cyclic adenosine 3',5'-monophosphate-8-14C by isolated, perfused rat liver.


The inhibition by physiological orthophosphate concentrations of hydrolytic and synthetic activities of liver glucose 6-phosphatase.


Some properties of an apoceruloplasmin-like protein in human serum.


Control of gluconeogenesis in liver. IV. Differential effects of fatty acids and glucagon on ketogenesis and gluconeogenesis in the perfused rat liver.


Membranes of animal cells. IV. Lipids of the L cell and its surface membrane.


A neurotoxin, toxin alpha, from Egyptian cobra (Naja haje haje) venom. I. Purification, properties, and complete amino acid sequence.


Anaerobic oxidation-reduction titrations of fungal laccase. Evidence for several high potential electron-accepting sites.


Reduced 1,6-dihydrodiphosphopyridine nucleotide. Chemical properties and enzymatic modification.


Studies on the activation of phosphorylase in skeletal muscle by contraction and by epinephrine.


Ketogenesis and cholesterol synthesis in normal and neoplastic tissues of the rat.


Steroidogenesis in a case of hydatidiform mole.


Micro-determination of corticosteroids in ovine peripheral plasma: effects of venipuncture, corticotrophin, insulin and glucose.


Management of craniopharyngioma in childhood.


Lipolytic action of serotonin in brown adipose tissue in vitro.


Excess dietary zinc for the maternal rat, and zinc, iron, copper, calcium, and magnesium content and enzyme activity in maternal and fetal tissues.


Renin-like pressor material in ovarian venous blood of pregnant rabbits.


The use of radiofibrinogen in the evaluation of the cold areas of the hepatic scans.


Herpes simplex virus infection detected on routine gynecologic cell specimens.


Reversed action of acetylated corticotrophin: inhibition of adreno-cortical secretion.


Effect of epinephrine on glucose metabolism in Novikoff ascites hepatoma cells.


Oestrogen treatment in Cushing's syndrome.


Occurrence of encephalitis caused by infectious bovine rhinotracheitis virus in calves in hungary.


Occurrence of influenza A-equi-2 infection of horses in hungary.


Gingival hyperplasia related to treatment with diphenylhydantoin (diphedan; DH).


Epinephro-phlebography of benign tumours.


Scintigraphy and angiography in demonstration of hepatic tumours.


Tumor extent as a determining factor in radiotherapy of glioblastomas.


Primary signet ring cell carcinoma of the urinary bladder. Report of two cases.


Haemodynamic studies in calves sustained on artificial hearts.


Effects of anticonvulsant drugs on nerve terminals.


The developing animal as a model.

The developing animal as a model.


The role of biochemical and neurohumoral factors in the laboratory evaluation of antiepileptic drugs.


Biology and morphology of glioblastoma multiforme.


Relation of laboratory evaluation to clinical effectiveness of antiepileptic drugs.


The relationship of optic nerve fiber groups activated by electrical stimulation to the consequent central postsynaptic events.


Roentgen sensitivity of cerebral tumours and so-called late irradiation necrosis of the brain.


Extraocular muscle intracellular and motor nerve responses to semicircular canal stimulation.


An examination of variability of blood chemistry, hematology, and proteins, in relation to age.


Nephroblastoma in a cat: the effects of nephrectomy and occlusion of the caudal vena cava.


Mosquito control activities of the U.S. armed forces in the republic of Vietnam.


Adenyl cyclase and hormone action. I. Effects of adrenocorticotropic hormone, glucagon, and epinephrine on the plasma membrane of rat fat cells. A large number of hormones, of diverse molecular structure, evoke characteristic responses in target cells via the intermediary 3',5'-AMP, the specificity of hormone action upon cell type being achieved by selective stimulation of adenyl cyclase. In the fat cells of rat adipose tissue, adenyl cyclase is stimulated by a number of hormones of disparate molecular structure, posing the question whether this cell type posesses multiple cyclase systems with distinctive specificities for individual hormones, or a single cyclase with broad specificity to a variety of hormones. Studies of the stimulatory effects of adenocorticotropin, glucagon, and epinephrine upon the adenyl cyclase of the rat fat cell "ghosts" (plasma membrane sacs) have shown that distinctive selectivity sites for each of these hormones can be differentiated. The beta-adrenergic blocking agent Kö 592 abolished the stimulatory effect of epinephrine without influencing adenocorticotropin or glucagon; Ca was required for adenocorticotropin action, but not for glucagon or epinephrine. Dose-response curves show that the affinity of hormones to the cyclase system was in the order: glucagon > adenocorticotropin >> epinephrine; the magnitude of cyclase activation by maximal doses of hormones had a reversed order. Combinations of maximal doses of hormones failed to produce additive stimulation. The results show that in the membrane of the fat cell a single catalytic unit of adenyl cyclase is coupled to distinctive selectivity sites for three lipolytic hormones.


Rapid transformation of cells by Rous sarcoma virus. The conditions for synchronous transformation of chick embryo cells by infection with Rous sarcoma virus are studied. Two factors, the treatment of cells with DEAE-dextran and the use of cells which grow rapidly following virus infection, are found to be most important. Under the conditions described, the Schmidt-Ruppin strain of Rous sarcoma virus at a multiplicity of higher than 5 induces morphological alteration in about 90 per cent of the cell population within 24 hours after infection. The alteration in the morphology is accompanied by acquisition of the ability of cells to grow in agar medium and by the increased rate of incorporation of thymidine and uridine into nucleic acids.


On the mechanism of action of adrenocorticotropic hormone: the binding of cyclic-3',5'-adenosine monophosphate to an adrenal cortical protein. The binding of cyclic 3',5'-adenosine monophosphate (cyclic AMP) within the adrenal cortical cell was studied. Cyclic AMP binds specifically to a protein which is associated predominantly with the microsomal fraction of the cell. The binding protein was purified approximately 100-fold.


Vitamin D3-stimulated template activity of chromatin from rat intestine. Administration of vitamin D to rats deficient in this vitamin markedly increases the template activity for DNA-dependent RNA synthesis by rat intestinal mucosal chromatin. The maximum stimulation of template activity occurs three hours after a dose of 2000 international units of vitamin D(3) is given. These results support the concept that vitamin D functions by initiating the transcription of DNA into mRNA, which codes for functional protein(s) involved in calcium transport in the small intestine.


Metabolism of oestriol-3-sulphate-16-glucosiduronate in pregnant women.


Corticosteroids in human blood. 3. Free and conjugated 17-hydroxycorticosteroids in healthy people and patients with Cushing's syndrome, before and after administration of corticotrophin.


Nuclear shape variations in the fascicular zone of the rat adrenal gland. I. Effect of corticotrophin.


Chromophobe pituitary adenoma with acromegaly and TSH-induced hyperthyroidism associated with parathyroid adenoma. Acromegaly and parathyroid adenoma.


Diagnosis of Cushing's syndrome. The differentiation between adrenocortical hyperplasia and adrenocrotical adenoma by means of lysine-vasopressin.


Glycogen content of leukocytes of some animal species.


Generation and survival of angiotensin in nonrefrigerated plasma. An explanation for presence of pressor material in human plasma protein solutions used clinically.


An improved system for the separation of metabolites of isotopically labelled vitamin D3 on silicic acid columns.


Clearing-factor lipase in adipose tissue. Distinction of different states of the enzyme and the possible role of the fat cell in the maintenance of tissue activity. 1. Incubation of intact epididymal adipose tissue from fed rats at 37 degrees in an albumin solution at pH7.4 in vitro results in rapid loss of clearing-factor lipase activity until a low activity, stable to prolonged incubation, is attained. The clearing-factor lipase activity of intact tissue from starved rats, which is initially much less than that of tissue from fed rats, is mainly stable to incubation at 37 degrees . 2. Much of the clearing-factor lipase activity of intact epididymal adipose tissue from fed rats is inactivated by collagenase. The enzyme activity of intact tissue from starved rats is not inactivated by collagenase. 3. The clearing-factor lipase activity of fat cells isolated from the epididymal adipose tissue of fed rats is stable to prolonged incubation at 37 degrees . It represents only a small proportion of the total activity of the intact tissue. In starved rats, the isolated fat cells contain a much higher proportion of the activity of the intact tissue. Their activity is also stable at 37 degrees . 4. Incubation of isolated fat cells in a serum-based medium leads to a progressive rise in clearing-factor lipase activity. Actinomycin increases the extent of this rise in activity. No rise in clearing-factor lipase activity occurs when stromal-vascular cells isolated from epididymal adipose tissue are incubated in the medium. 5. The findings indicate that less than 20% of the activity of intact adipose tissue from fed rats is retained when fat cells are isolated from the tissue by collagenase treatment. The activity that is lost could be that which normally functions in the uptake of triglyceride fatty acids by the tissue.


Effect of actinomycin D and guanidine on the formation of a ribonucleic acid polymerase induced by foot-and mouth-disease virus and on the replication of virus and viral ribonucleic acid. The RNA-dependent RNA polymerase induced in baby-hamster kidney cells by infection with foot-and-mouth-disease virus can be detected as early as 60min. after infection, which is 60min. before viral RNA synthesis commences. The time at which the polymerase can first be detected coincides with the latest time at which actinomycin D (50mug./10(7) cells) or guanidine (1mg./10(7) cells) inhibits virus replication. However, by increasing the concentration of guanidine, viral replication can be inhibited later in the growth cycle, casting doubt on the validity of the hypothesis that guanidine acts specifically on the formation of the viral RNA polymerase.


The adenosine triphosphate inhibition of the pyruvate kinase reaction and its dependence on the total magnesium ion concentration. 1. The effects of ATP, PP(i) and EDTA on the skeletal-muscle pyruvate kinase reaction at various concentrations of magnesium (where ;magnesium' refers to total Mg(2+), both free and in the form of complexes) were investigated. The reaction rate was determined as the amount of pyruvate formed in a recorded time of incubation. 2. At 44mm-magnesium the K(m) values for ADP and phosphoenolpyruvate were unaltered by the presence of ATP up to 6.8mm in systems buffered with either tris-hydrochloric acid or glycylglycine-sodium hydroxide, but the K(m) values were different in these systems. The K(m) for one substrate was independent of the concentration of the second substrate. 3. At 10mm-magnesium in the tris-hydrochloric acid system ATP inhibited the reaction competitively with respect to ADP and phosphoenolpyruvate. In the glycylglycine-sodium hydroxide system the inhibition appeared to be non-competitive. At 10mm-magnesium the K(m) values were lower than at 44mm-magnesium and dependent on the system used. 4. In the tris-hydrochloric acid system the reaction rate rose with increasing magnesium concentration up to a maximum at a concentration 10-20 times that of ADP. Further increase inhibited the reaction and at 44mm-magnesium the rate was 25-50% of its maximum. This inhibition paralleled that produced by increasing trimethylammonium chloride concentrations and was not due to a specific effect of the Mg(2+) ion. 5. In the presence of 6.8mm-ATP no reaction occurred below 4-6mm-magnesium, and further increase apparently abolished the inhibition as the reaction rate increased and became equal to those obtained in the absence of ATP at 10-25mm-magnesium. Further increase in magnesium concentration gave reaction rates that were slightly higher in the presence of ATP than in its absence. The maximal rate in the presence of ATP was distinctly lower than in its absence. When 6.8mm-PP(i) or 6.8mm-EDTA was present the variations in reaction rate with rising magnesium concentration were similar to that obtained in the presence of ATP below 6-8mm-magnesium but further increase in the magnesium concentration resulted in an increase in the rate up to a maximum comparable with that of the control. The effect of pure chelation was thus a displacement of the reaction maximum to higher magnesium concentrations without changing the maximal rate. When correction had been made for this effect, ATP gave inhibition at 44mm-magnesium that was competitive with respect to ADP (K(i) 2.1x10(-2)m). This degree of inhibition is far less than was reported earlier and its importance for the mechanism of the pyruvate kinase reaction is discussed.


Virus antibody levels in multiple sclerosis.


Mouse hepatitis virus (MHV3) infection in chronic murine schistosomiasis mansoni.


The role of hemodynamic factors on urinary calcium and magnesium excretion.


A simple method for separate determination of the main 17,21-dihydroxy-20-ketosteroids.


Urinary levels of testosterone and epitestosterone in Down's syndrome (mongolism).


Erythrocyte 2, 3-diphosphoglycerate mutase: an optical test in hemolysates.


Implications and applications of emission and atomic absorption spectroscopy for biological problems.


Peptidase in the cornea.

Peptidase in the cornea.


Collagenase in the cornea.

Collagenase in the cornea.


Transmission of mammary tumor virus by female GR mice: results of egg transplantation.


Respiration and protein synthesis in Escherichia coli membrane-envelope fragments. I. Oxidative activities with soluble substrates. This paper describes experiments conducted with membranous and soluble fractions obtained from Escherichia coli that had been grown on succinate, malate, or enriched glucose media. Oxidase and dehydrogenase activities were studied with the following substrates: nicotinamide adenine dinucleotide, reduced form (NADH), nicotinamide adenine dinucleotide phosphate, reduced form (NADPH), succinate, malate, isocitrate, glutamate, pyruvate, and alpha-ketoglutarate. Respiration was virtually insensitive to poisons that are commonly used to inhibit mitochondrial systems, namely, rotenone, antimycin, and azide. Succinate dehydrogenase and NADH, NADPH, and succinate oxidases were primarily membrane-bound whereas malate, isocitrate, and NADH dehydrogenases were predominantly soluble. It was observed that E. coli malate dehydrogenase could be assayed with the dye 2,6-dichlorophenol indophenol, but that porcine malate dehydrogenase activity could not be assayed, even in the presence of E. coli extracts. The characteristics of E. coli NADH dehydrogenase were shown to be markedly different from those of a mammalian enzyme. The enzyme activities for oxidation of Krebs cycle intermediates (malate, succinate, isocitrate) did not appear to be under coordinate genetic control.


Choline metabolism and membrane formation in rat hepatoma cells grown in suspension culture. II. Phosphatidylcholine synthesis during growth cycle and fluctuation of mitochondrial density. The incorporation of methyl-labeled choline into phosphorylcholine and phosphatidylcholine of cellular membranes by Novikoff rat hepatoma cells (line N1S1-67) during growth in suspension culture was investigated. Upon initiation of a fresh culture at 10(5) cells/ml, the rate of synthesis of phosphorylcholine by the cells was four to five times greater than that of the synthesis of phosphatidylcholine. While the rate of synthesis of the latter remained relatively constant, the rate of phosphorylation of choline decreased progressively during the course of the growth cycle of the culture to 10-20% of the initial rate when the culture reached stationary phase at 3 x 10(6) cells/ml. The decrease in phosphorylcholine synthesis during the growth cycle was not due to depletion of choline in the medium or a decrease in its concentration, but was correlated with a decrease in choline kinase activity of the cells as measured in cell-free extracts. Newly synthesized phosphatidylcholine was detectable in cells only as an integral part of cellular membranes. Its distribution among various cytoplasmic membrane structures separated by isopycnic centrifugation in sucrose density gradients remained relatively constant during the growth cycle. About 50% was associated with the mitochondria, and the remainder with plasma membrane fragments and other membranous structures with mean densities of about 1.15 and 1.13 g/cm(3), respectively. However, the density of the mitochondria increased from about 1.167 g/cm(3) in early exponential phase cells to about 1.190 g/cm(3) in stationary phase cells. The finding that the density of the entire propulation of mitochondria changed simultaneously and progressively is in agreement with the view that mitochondria grow by addition of phospholipids and structural proteins and increase in number by division.


Japanese encephalitis in South India. A summary of recent knowledge.


Blood groups and lung cancer.

Blood groups and lung cancer.


Salivary gland inclusion disease in the tar- sier.


Low resolution study of crystalline L-lactate dehydrogenase.


Spontaneous lesions in a colony of Chinese hamsters.


Isolation and characterisation of the glycopeptides of the structural glycoprotein of corneal stroma.


Facial palsy: a medical emergency.

Facial palsy: a medical emergency.


Adenosine-3',5'-phosphate: identification as acrasin in a species of cellular slime mold. Acrasin, the chemotactic agent responsible for aggregation in thecellular slime molds, has been identified for one species, Dictyostelium discoideum, as adenosine-3', 5'-phosphate.


Synthesis of a model relating to the chromophores of capreomycin and viomycin.


The preferential loss of the polylysine- or polyornithine-stimulated activity of Clostridium perfringens polynucleotide phosphorylase during proteolysis. 1. An improved method for the purification of Clostridium perfringens polynucleotide phosphorylase (nucleoside diphosphate-polyribonucleotide nucleotidyltransferase, EC 2.7.7.8) is described. The product was stable and was highly stimulated by polylysine or polyornithine. 2. It migrated as a single enzyme during sucrose-density-gradient centrifugation, and no separation of polymerization and phosphorolytic activities was observed. 3. Trypsin digestion caused a rapid, preferential loss of the polylysine- or polyornithine-stimulated activity, which was prevented by low concentrations of polyornithine. 4. The protection by polyornithine was not specific. 5. It is concluded that charge effects on the clostridial polynucleotide phosphorylase itself are primarily responsible for the stimulation of this enzyme by polylysine or polyornithine.


The influence of the size and nature of basic activators on Clostridium perfringens polynucleotide phosphorylase-catalysed polyadenylic acid synthesis. 1. Basic oligo- and poly-(amino acids) stimulate polyadenylic acid synthesis by purified Clostridium perfringens polynucleotide phosphorylase (nucleoside diphosphate-polyribonucleotide nucleotidyltransferase, EC 2.7.7.8). 2. The effectiveness of the activators increases with chain length up to approx. 20-30 residues. 3. Polymers of the l and dl series are equally effective on a weight-for-weight basis. 4. l-Lysine, d-lysine, diethylamine and triethylamine, as hydrochlorides or hydrobromides, all stimulate the reaction markedly if their concentration is high enough. Their effect is similar to that of sodium chloride. 5. The size of the product depends primarily on the Mg(2+) concentration and basic polymers have a relatively limited effect on it. 6. Polyadenylic acid itself undergoes an Mg(2+)-catalysed non-enzymic hydrolysis.


The conversion of steroid 7,9-dienes into cholesterol.


Biosynthesis of abscisic acid: incorporation of radioactivity from [2-14C]mevalonic acid by intact fruit.


Comparative epidemiological features of Japanese encephalitis in the Republic of Korea, China (Taiwan) and Japan. The epidemiology of Japanese encephalitis in the Republic of Korea from 1949 to 1966 is described and comparisons are made with the situations in Japan and China (Taiwan). Some similarities and some differences are noted. Recent epidemics in Korea coincided with those in southern Japan but the annual fluctuations were more distinct in Korea. The disease mainly affected children in Korea and, in contrast to the situation in Japan, persons in the older age-groups were rarely affected. The authors also discuss the geographical pathology of Japanese encephalitis in Korea.


Trsmission of smallpox by contact and by aerosol routes in Macaca irus. Smallpox is believed not to occur naturally in species other than man. However, reports of several epizootics of an exanthematous disease, similar to smallpox, in wild monkeys have raised the question of a simian reservoir. If such a reservoir for smallpox exists, the eradication of this disease from the world would be a difficult or impossible task. Transmission of smallpox in Macaca irus has been studied to determine whether transmission occurs and if infection chains can be maintained by this species.Transmission was consistently accomplished by both contact and aerosol routes. In the contact transmission studies, the smallpox infection was maintained through 6 passages but was lost with the seventh passage. The virulence of the virus did not appear to increase as the virus was serially passed in monkeys. Continuing studies of the possible occurrence of smallpox and of monkeypox in simian populations are warranted.


Active transport of Cl- across the isolated intestinal mucosa of Testudo hermanni.


Superinfection of tumors with viruses.

Superinfection of tumors with viruses.


Electron histochemical evidence of different types of mossy fibre endings in the cerebellar cortex.


The involvement of cyclic 3',5'-adenosine monophosphate in the growth hormone release mechanism (s).


Transcarbamylase activity and Na2H32PO4 transport in mammalian cells in culture.


Amoebiasis in India.

Amoebiasis in India.


Chondromatosis: report of case.

Chondromatosis: report of case.


Renin-angiotensin-aldosterone system in hypertension. The clinical usefulness of measuring components of thisystem.


The effect of nitrogen starvation on Tenebrio molitor L.


[Follow-up study of radial nerve injury. A review of 213 cases].


Factors in the maturation of the adrenal.


Facilitatory and inhibitory influences on the pituitary-adrenal system: a study with chlorpromazine.


The cental nervous system and pituitary-adrenocortical feedback mechanisms.


Gastric mucus and the gastric mucous barrier. A review.


Myoclonic encephalopathy of infants. A report of two cases and observations on related disorders.


Atrial septal defect: results of repair in adults.


Sensitivity of chick embryos to chlorpromazine.


Studies on the solubilization of 2',3'-cyclic nucleotide 3'-phosphohydrolase from bovine brain.


Studies on in vitro RNA synthesis in human brain tumor nuclei.


Projections of the slow-conducting myelinated fibers of the splanchnic nerve on the motor cortex of the cat.


Sustained evoked potential changes of the spinal cord.


Some factors modulating neural activities in peripheral auditory centers.


Steroid-sensitive neurones in rat brain: anatomical localization and responses to neurohumours and ACTH.


Malignant non-chromaffin paraganglioma of thigh involving bone locally.


Studies on the ability of compounds to block the diabetogenic activity of streptozotocin.


Glomus jugulare tumors.

Glomus jugulare tumors.


Release of acetylcholine at voluntary motor nerve endings by H. H. Dale, W. Feldberg, M. Vogt.


Relation between electrical changes during nerve activity and concentration of choline esterase by D. Nachmansohn and B. Meyerhof.


Effects of lowered muscle temperature upon neuromuscular blockade in man by Eleanor Zaimis, Thomas H. Cannard, and Henry L. Price.


Mode of action of streptolydigin.

Mode of action of streptolydigin. Streptolydigin and rifamycin inhibit the catalytic function of ribonucleic acid (RNA) polymerase. Streptolydigin can inhibit polymerization after the reaction has started, whereas rifamycin is effective only if it is preincubated with RNA polymerase prior to the addition of substrates. The same relationships are observed with respect to these two antibiotics if the nucleoside triphosphate-pyrophosphate exchange reaction is used in the assay system. The inhibitory effect of streptolydigin is reversible by further addition of RNA polymerase but not by addition of deoxyribonucleic acid to the assay system.


Histamine-sensitizing factor, mouse-protective antigens, and other antigens of some members of the genus Bordetella. The three species of the genus Bordetella-B. pertussis, B. parapertussis, and B. bronchiseptica-have many antigens in common. Studies on representative strains of these species have shown that there are only a few specific antigens in each species. Whole-cell vaccines and extracts from B. pertussis contained specific mouse-protective antigen and a histamine-sensitizing factor. In addition, whole-cell vaccines and some saline extracts protected mice against intracranial challenge with B. bronchiseptica. Cells and a saline extract of B. parapertussis also protected against B. bronchiseptica but not against B. pertussis. Whole cells of B. bronchiseptica protected against B. bronchiseptica, but only one of three saline extracts protected against this challenge. Neither whole cells nor saline extracts from B. bronchiseptica protected against B. pertussis. The antigen in B. pertussis responsible for cross-protection against B. bronchiseptica was less resistant to heat than the protective antigen in B. bronchiseptica. Since histamine-sensitizing factor was not detected in B. bronchiseptica or B. parapertussis cells or extracts, this factor is not required to protect mice against B. bronchiseptica challenge. Whether B. pertussis vaccines protected against B. bronchiseptica by a nonspecific mechanism was not established, but it is clear that the specific antigen responsible for protection against B. pertussis was found only in B. pertussis and not in B. bronchiseptica or B. parapertussis.


Once-a-day neurally stimulated and basal ACTH secretion phases in man and their response to corticoid inhibition.


Effect of adrenocorticotropin on desoxycorticosterone, corticosterone and aldosterone excretion.


Guinea pig adrenal 3-beta-hydroxysteroid dehydrogenase: is there more than one enzyme?


Neurotransmission in central nervous tissue: a study of isolated rabbit retina.


Entero-and myxoviral infections of the central nervous system.


Structural difference in sites on the surface membrane of normal and transformed cells.


Iprindole: an antidepressant which does not block REM sleep.


Immune complexes in mice infected neonatally with Moloney leukaemogenic and murine sarcoma viruses.


Dietary iodine and goiter in Ceylon.

Dietary iodine and goiter in Ceylon.


Virus abortion in california.

Virus abortion in california.


Foot-and-mouth disease in man--notes on a recent case.


Screening of food handlers.

Screening of food handlers.


Differential diagnosis of avian viral respiratory diseases: a comparison of fluorescent antibody and virus isolation techniques.


Determination of the ability of streptomycetes to utilize carbon sources by the method of culturing on silica gel.


Liver 3':5'-nucleotide phosphodiesterase and its activity in rat livers perfused with insulin.


Enzyme histochemistry of the pars intermedia of ox pituitary gland.


Cilia in cell-cultured fibroblasts. I. On their occurrence and relative frequencies in primary cultures and established cell lines.


A method for extraction and chromatographic isolation, purification and identification of tetrahydrocannabinol and other compounds from marihuana.


Sleep.

Sleep.


Response of rat adrenal tissue to ACTH in a flowing system.


Effects of chemical and enzymatic modifications of glucagon on its activation of hepatic adenyl cyclase.


Properties of the corticotrophin-releasing factor of the rat median eminence.


Role of cyclic AMP in mediating the effects of MSH, norepinephrine, and melatonin on frog skin color.


Interrelationships between thyrocalcitonin, parathyroid hormone and vitamin D: control of serum calcium in hypervitaminosis D.


Hypothalamic control of ACTH release from ectopic pituitary glands.


Long-term feeding studies of methyl ethyl cellulose ('Edifas' A) and sodium carboxymethyl cellulose ('Edifas' B) in rats and mice.


2-Thiophenecarboxylic acid: a hypoglycemic, antilipolytic agent with hypocalcemic and hypophosphatemic effects in rats.


Synergism in carcinogenesis.

Synergism in carcinogenesis.


Carcinogenicity of sterigmatocystin.

Carcinogenicity of sterigmatocystin.


Free radicals in irradiated single crystals of 5-halo-uracil derivatives: 5-bromouracil, 5-bromodeoxyuridine and 5-iododeoxyuridine.


Schwann-cell proliferation in chronic neuropathies.


Role of interferon in replication of virulent and attenuated strains of measles virus.


Rescue of Rous sarcoma virus from virogenic mammalian cells associated with chicken cells and treated with Sendai virus.


Electrophoretic properties of foot-and-mouth disease virus strains and the selection of intra-strain mutants.


A host-cell DNA function involved in the replication of avian tumour viruses and of fowl-plague virus.


Activation of a mammary tumour virus in O20 strain mice by x-irradiation and urethane.


Antiviral and cytotoxic effects of mycophenolic acid.


The structure of foot-and-mouth disease virus protein.


Studies on mitochondrial tRNA from animal cells. I. A comparison of mitochondrial and cytoplasmic trna and aminoacyl-tRNA synthetases.


Methylation of mammalian DNA: studies on Novikoff hepatoma cells in tissue culture.


Heterogeneity of polyoma virus DNA: isolation and characterization of non-infectious small supercoiled molecules.


Some aspects of the pharmacology of valyl-beta-(pyrazolyl-3)-L-alanyl6-angiotensin II.


Potentiation of the anticalorigenic action of the adrenergic beta-receptor antagonist MJ 1999 by cyclic 3',5'-AMP.


The effect of alpha-methyl-para-tyrosine on sleep patterns of the monkey.


Cofactor requirements for metabolism of alpha-amino-n-butyric acid by brain homogenates.


Deoxycytidine kinase. I. Distribution in normal and neoplastic tissues and interrelationships of deoxycytidine and 1-beta-D-arabinofuranosylcytosine phosphorylation.


Phospholipid metabolism and adrenal medullary activity. I. The effect of acetylcholine on tissue uptake and incorporation of orthophosphate-32P into nucleotides and phospholipids of bovine adrenal medulla.


Human brain protein, drugs and dreams.

Human brain protein, drugs and dreams.


The effect of Ca++ omission on the secretion of catecholamines and the incorporation of orthophosphate-32P into nucleotides and phospholipids of bovine adrenal medulla during acetylcholine stimulation.


Induction of rat kidney tumours by ethyl methanesulphonate and nervous tissue tumours by methyl methanesulphonate and ethyl methanesulphonate.


Remitting demyelinating disease associated with myeloproliferative syndrome and histiocytosis of the spleen.


Longstanding haemochromatosis with polyarthritis and terminal hepatoma.


Insulinoma presenting with episodic attacks of transient unconsciousness.


The value of mercury perchloride and other agents in reducing tumour recurrence in colon anastomosis.


Malignant disease in juveniles.

Malignant disease in juveniles.


Irrigation via self-retaining rectal tube in prevention of suture-line recurrent carcinoma.


Diphosphonates inhibit formation of calcium phosphate crystals in vitro and pathological calcification in vivo. Two diphosphonates containing the P-C-P bond, CH(3)C(OH)(PO(3)HNa)(2) and H(2)C(PO(3)HNa)(2), inhibit the crystallization of calcium phosphate in vitro and prevent aortic calcification of rats given large amounts of vitamin D(3). The diphosphonates therefore have effects similar to those described for compounds containing the P-O-P bond but are active when administered orally.


Neuropathological studies on myelomatosis.


Two studies of childhood dreaming.

Two studies of childhood dreaming.


Roentgenologic studies of cerebrospinal fluid formation in the dog.


Lipid embolism to the brain following lymphography. Case report and experimental study.


Vascular structure of glioblastomas.

Vascular structure of glioblastomas.


Mammographic technique: need for routine spot roentgenograms.


Effects of insecticides on mammalian cells and virus infections.


The significance of simian viruses to the monkey colony and the laboratory investigator.


Recent advances in the study of simian viruses.


Subhuman primates in experimental viral oncology.


Diseases of primates affecting the choice of species for toxicologic studies.


Fluorescent-antibody studies with antisera against heated and unheated poliovirus type 1. Fluorescent-antibody (FA) reagents were prepared from sera of guinea pigs immunized with either native infectious poliovirus type 1 or poliovirus type 1 which had been heated at 56 C for 30 min. Conjugates made from sera of animals immunized with heated virus gave higher direct FA staining titers on air-dried, acetone-fixed, infected cells than conjugates made from sera of animals immunized with native infectious virus. Evidence was obtained that complement-fixing antibody reactive with heated antigen was responsible for the FA staining. Two conjugates prepared from sera of guinea pigs immunized with heated poliovirus type 1 were successfully used to identify 21 type 1 viruses isolated from a group of 44 stool suspensions studied as unknowns. These conjugates did not stain any of 23 heterologous enteroviruses present in the remainder of the stools and gave minimal non-specific staining.


In vitro antiviral activity of mycophenolic acid and its reversal by guanine-type compounds. With the agar diffusion test and BS-C-1 cells, mycophenolic acid was found to give a straight-line dose-response activity in inhibiting the cytopathic effects of vaccinia, herpes simplex, and measles viruses. Plaque tests have shown 100% reduction of virus plaques by mycophenolic acid over drug ranges of 10 to 50 mug/ml and virus input as high as 6,000 plaque-forming units (PFU) per flask. Back titration studies with measles virus inhibited by mycophenolic acid have indicated that extracellular virus titers were reduced by approximately 3 logs(10) and total virus was reduced by 1 log(10). The agar diffusion test system lends itself readily to drug reversal studies. Mycophenolic acid incorporated into agar at 10 mug/ml gave 100% protection to virus-infected cells. Filter paper discs impregnated with selected chemical agents at concentrations of 1,000 mug/ml (20 mug per filter paper disc) were placed on the agar surface. Reversal of the antiviral activity of mycophenolic acid was indicated by virus breakthrough in those cells in close proximity to the filter paper disc. Chemicals showing the best reversal of the antiviral activity of mycophenolic acid were guanine, guanosine, guanylic acid, deoxyguanylic acid, and 2,6-diaminopurine. The reversal of antiviral activity was confirmed by titrations of virus produced with various amounts of both mycophenolic acid and guanine present and by isotope tracer methods with uptakes of labeled uridine, guanine, leucine, and thymidine in treated and nontreated, infected and noninfected cells as parameters. All antiviral effects of mycophenolic acid at 10 mug/ml could be reversed to the range shown by untreated controls by the addition of 10 mug/ml of those chemicals exhibiting reversal activity.


Insulinoma in childhood.

Insulinoma in childhood.


Variation in the size of the kidney during nephro-angiography in anaesthetised dogs.


Biosynthesis of aspartic, glutamic, gamma-aminobutyric acids and glutamine in brain of rats deprived of total sleep or paradoxical sleep.


Evaluation of nerve injuries by evoked potentials and electromyography.


The effect of antioxidant-deficiency on phospholipid composition in the chick brain.


Regional cerebral blood flow in patients with intracranial tumors.


Ultrasonic echo examination as an aid in diagnosis of congenital bile duct lesions.


Triglyceride sub-classes of dog plasma and organs.


Physical studies of lipid-lipid and lipid-protein interactions.


The relation of carnitine to the formation of phosphatidyl-beta-methylcholine by Tenebrio molitor L. larvae.


Skin lesions of the breast.

Skin lesions of the breast.


Food-and-mouth disease viremia.

Food-and-mouth disease viremia.


Immunogenicity of nanogram to milligram quantities of inactivated foot-and-mouth disease virus. II. Comparative response of guinea pigs and steers.


Outbreak of malignant catarrhal fever in kansas.


Electron microscopic study on RNA of cytoplasmic polyhedrosis virus of the silkworm.


Inhibition of poliovirus replication by N-methylisatin-beta-4':4'-dibutylthiosemicarbazone.


The effect of magnesium on the intracellular crystallization of rhinovirus.


Multiplicity of viral genomes in an SV40 transformed hamster cell line.


Selective failure of protein synthesis in herpesvirus-infected cells deprived of arginine.


The metabolism of small and of large amounts of progesterone in man.


Immunological studies on the membrane systems of cancer cells. I. Immunochemical analysis of membrane fractions from cancer cells.


Desynchronized sleep during human ontogenesis.


Vigilance changes in rats with different levels of nonspecific excitability.


Are stages of sleep really necessary?

Are stages of sleep really necessary?


The nature of all night sleep patterns.

The nature of all night sleep patterns.


Quantitative representation of the course of sleep based on the frequency distribution of EEG patterns.


Relation of sleep onset to rapid eye movement sleep.


Waking fantasies following interrupted and completed REM periods.


Penile erections during sleep.

Penile erections during sleep.


Tourniquet poditis--an experimental pedal inflammation in the rat.


Immunohistological studies of mice infected with bordetella pertussis.


Entamoeba histolytica-like amoebae occurring in man. Six Entamoeba histolytica-like amoebae of the Laredo-type are compared with classical E. histolytica strains with regard to morphology, temperature-tolerance, response to hypotonic solutions, antigenic make-up, pathogenicity, sensitivity to drugs, and biochemistry. Sharp discontinuities are shown to exist between the 2 groups in some parameters, sufficient, under certain conditions, to justify creation of a new species designation for the Laredo-type amoebae. Nevertheless, this action is not recommended at the present time because of the small number of "aberrant" strains so far isolated, and because of the limited comparative studies so far carried out.Entamoeba moshkovskii is compared with the Laredo-type E. histolytica. No consistent distinction can be made between the 2 groups except for the sources of the strains-the former is free-living and the latter parasitic. It is therefore suggested that E. moshkovskii may actually be a parasite of the Laredo type, only incidentally encountered in free-living environments because of its wide temperature and tonicity tolerances. Since, here too, only limited studies are so far available, it is suggested that the term "moshkovskii-like E. histolytica" be used until sufficient data are accumulated to assign these amoebae to a more precise location in the taxonomic scheme.


Melanotic neuroectodermal tumour of infancy. Report of 2 cases.


Metabolic events associated with steroid biosynthesis in adrenal tissue cultures.


Failure to diagnose Addison's disease and value of tetracosactrin (Synacthen) stimulation test.


Synergistic and inhibitory activity of various hormones on the exophthalmo producing factor in sera of patients suffering from endocrine exophthalmos.


The half-life of porcine corticotrophin in pigs.


Inhibitory effect of diphenylhydantoin on the feedback control of corticotrophin release.


Quantitative relationship of preexisting homotypic antibodies to excretion of poliovirus types 1, 2, and 3 following the feeding of trivalent attenuated poliovirus vaccine.


Rapid fluorometric analysis of plasma corticosteroids as a test of adrenocortical function in the dog.