Management of schizfphrenia in the community.


Biochemical and quantitative electron microscopic evidence for heterogeneity of mitochondria from Saccharomyces cerevisiae.


High resolution autoradiographic studies of the uptake of 3H-5-uridine into condensed and dispersed chromatin of nuclei and granular and fibrillar components of nucleoli of Novikoff hepatoma ascites cells.


Nutritional requirements of mouse adrenal cortex tumor cells in culture.


The complete and centralised treatment of paraplegia.


The inhibition of mevalonic kinase by geranyl and farnesyl pyrophosphates.


Studies of sterility of calf venous blood.


Hepatoma and groundnuts in the West Nile district of Uganda.


Simultaneous bilateral nephrectomy and renal allotransplantation for bilateral Wilms' tumor.


Somnolence in rhesus monkey following intracerebral inoculation.


Tartaric acid metabolism. 8. Crystalline tartronic semialdehyde reductase.


Alterations in lactate dehydrogenase of the brain, heart, skeletal muscle, and liver of rats of various ages.


Enzymatic desaturation of stearyl acyl carrier protein.


Hypopituitarism associated with cavernous sinus thrombosis. Report of a case.


The release of adenosine triphosphate from frog skeletal muscle in vitro. 1. Active frog sartorius muscle in vitro liberates a substance into the bathing solution which has a pronounced stimulatory action on the frog heart.2. The stimulatory effect is not due to an increase in the K(+) concentration of the bathing solution, nor is it due to the liberation of catecholamines.3. In a molecular sieve chromatography procedure the stimulatory substance can be eluted in a single fraction which shows a maximum absorption of U.V. light at a wave-length of 265 nm, indicative of the presence of substances containing a purine ring.4. Low concentrations (10(-7)-10(-8) g/ml.) of adenosine triphosphate (ATP), adenosine diphosphate (ADP) and uridine triphosphate (UTP) have a marked stimulatory action on the frog heart. The action of ATP and ADP on the heart is qualitatively very similar to that of the muscle bathing solution, while the action of UTP is distinctly different. The triphosphates of inosine, cytidine and guanosine stimulate the heart when in high concentration only. Adenosine and adenosine monophosphate do not stimulate the heart.5. Incubation of the muscle bathing solution and of solutions of ATP with the enzyme apyrase for the same time produces a similar marked reduction in the stimulatory action of both on the heart. Apyrase catalyses the break-down of nucleotide triphosphates to monophosphates.6. The elution behaviour of the stimulatory substance determined by molecular sieve chromatography is the same as that for ATP.7. The muscle bathing solution causes light to be emitted from firefly lantern extract, the pattern of light emission being similar to that produced by nucleotide triphosphates.8. The concentrations of ATP having the same quantitative action on the frog heart and on firefly extract as a given muscle bathing solution are almost identical, whereas the matching concentrations of ADP and UTP in the two methods of assay are widely different.9. It is concluded that ATP is released from active frog skeletal muscle in vitro. This release may play an important part in the reactive hyperaemia of muscular exercise since ATP has a powerful vasodilator action.


Use of bone age in the diagnosis of short children.


A study of the mechanism of quantal transmitter release at a chemical synapse. 1. The nerve-muscle preparation of the cutaneous pectoris of the frog has been used to study quantal transmitter release.2. When the osmotic pressure of the external solution is raised 1.5-2 fold, the frequency of miniature end-plate potentials (m.e.p.p.s) rises by 1.5-2 orders of magnitude. This effect is independent of the presence of Ca(2+) ions and of the nature of the substances by which the osmotic pressure has been increased.3. In Ca(2+) free hypertonic solution the nerve impulse still invades the nerve terminals but does not alter the frequency of the m.e.p.p.s.4. The arrival of the impulse in the terminals causes an immediate increase in the rate of quantal release, provided divalent cations are present whose passage through the axon membrane is facilitated by excitation (Ca(2+), Sr(2+), Ba(2+)).5. Divalent cations which penetrate only slightly (Mg(2+), Be(2+)) lower the frequency of m.e.p.p.s and suppress the end-plate potential (e.p.p.) evoked by an impulse, in the presence of Ca(2+) ions. Be(2+) is a more effective inhibitor than Mg(2+).6. In Ca(2+) free solutions, adding Mg(2+) causes an increase in the frequency of m.e.p.p.s evoked by depolarization of the nerve endings or by treatment with ethanol.7. The trivalent cation La(3+) is more effective than divalent cations are in increasing the frequency of m.e.p.p.s. The tetravalent cation Th(4+) also raises the m.e.p.p. frequency.8. The observations summarized in paragraphs 2-7 indicate that the frequency of m.e.p.p.s at a constant temperature depends only on the concentration of uni-, di- and trivalent cations inside the nerve ending. It is suggested that the internal cation concentration influences the adhesion between synaptic vesicles and the membrane of the nerve ending.9. For a model experiment, artificial phospholipid membranes have been used to study the effect of uni-, di-, tri- and tetravalent cations on the adhesion process. At pH 7-7.4, the time required for adhesion to take place decreases with increasing cation concentration in the bath. Ca(2+) ions are 100-1000 times more effective than K(+) ions; La(3+) and Th(4+) ions are still more effective. The ;adhesion time' decreases when the pH is lowered; it increases greatly with lowering of temperature.10. The hypothesis is put forward that the mutual adhesion of artificial vesicles made of phospholipid membranes, and the adhesion between synaptic vesicles and the membrane of the nerve ending arise by a common mechanism. In both cases, the important factor is the influence of cations on the electric double layer at the membrane surface.


Studies on the formation of hyperplastic focus by variola virus in human cell cultures. II. Virus growth and focus formation in HeLa cell cultures.


Herpes simplex virus DNA.

Herpes simplex virus DNA.


Distribution and translation of poliovirus RNA in guanidine-treated cells.


SV40 T antigen induction and transformation in human fibroblast cell strains.


Cellular interactions of several enteroviruses and a reovirus after treatment with sodium borohydride or carbohydrases.


The lipids of paramyxoviruses: a comparative study of Sendai and Newcastle disease viruses.


Some remarks on the morphology of virus particles of the B type and their isolation from mammary tumors.


In vitro cultivation of the mouse mammary tumor virus: replication of MTV in tissue culture.


Ultraviolet inactivation of MM virus RNA in terms of sodium chloride concentrations.


Use of mixed hemagglutination technique in detection of virus-induced antigen(s) on SV40-transformed cell surface.


Sulphatase activity in human epidermis and various tumours.


Two rare forms of tumour in the hypothalamo-hypophysial system. Infundibular choristoma and glioblastoma infiltrating the pituitary.


Structural and functional changes in rat kidney during CCl4 intoxication.


Studies on cellular autophagocytosis. The formation of autophagic vacuoles in the liver after glucagon administration.


Granular cell myoblastoma of the breast.


Synovial sarcoma of the anterior abdominal wall.


Plaque formation by 55 rhinovirus serotypes. Plaque production by 55 rhinoviruses in several lines of HeLa cells is reported. Forty-nine types produced macroscopic plaques under the conditions described previously employing 30 mM MgCl(2) and 30 mug/ml of DEAE-dextran in overlay and M HeLa cells. Basically three kinds of plaques were observed: clear large and intermediate sized plaques and small turbid plaques. Five rhinoviruses produced plaques only in a sensitive clonal line isolated from the M HeLa line. Rhinovirus type 4 produced plaques after the pretreatment of cells with actinomycin D. Enhancement of plaque formation by MgCl(2) has been demonstrated to date with 17 rhinoviruses. Some rhinoviruses were enhanced either by DEAE-dextran or by dextran sulfate in the overlay. No inhibitors were found in any of 10 bovine sera tested. Rhinovirus type 2 was visualized inside HeLa cells by electron microscopy and there appeared to be a very high ratio of physical particles per infectious unit of virus.


Activities of arginine dihydrolase and phosphatase in Streptococcus faecalis and Streptococcus faecium. Strains of Streptococcus faecalis and S. faecium are known to produce ammonia from arginine, but only S. faecalis couples the adenosine triphosphate (ATP) produced through the arginine dihydrolase pathway to growth processes. The specific activities of the arginine dihydrolase enzymes were found to be much lower in S. faecium (0.01 to 0.10) than in S. faecalis (0.24 to 1.60). Phosphatase activities in both strains were similar (up to 0.11), but equaled or exceeded the activities of the arginine dihydrolase enzymes in S. faecium. The failure of S. faecium to show increased growth in arginine media is explained on the basis of low activities of the arginine dihydrolase enzymes coupled with sufficient phosphatase activity to negate any benefit from ATP formed.


Methods for detecting food-borne enteroviruses. A method previously reported for detecting virus in a model system composed of cottage cheese contaminated with coxsackievirus type A9 has been adapted to detecting selected strains of enteroviruses in a variety of foods. Bentonite is omitted and serum is added for extracting virus from low-protein foods. Samples of foods, usually 25 g, must contain at least 3 to 4 plaque-forming units for a 50% probability of detecting virus. Sensitivity in detecting echovirus type 6 was lower than that for the other viruses used. After extraction from potato salad, poliovirus type 2 was completely reactivated if it had been neutralized with coproantibody, but it was only partially reactivated if neutralized with hyperimmune rabbit serum.


Production of reference enteroviruses.

Production of reference enteroviruses. Forty-five human enterovirus reagents of certified purity and quality were prepared for use as seed viruses and as immunizing antigens. One of the reagents was ampouled as "untreated" seed virus, whereas 14 were ampouled as "MgCl(2)-stabilized" reagents. The remaining 30 reagents were ampouled as "untreated" seed viruses and as "MgCl(2)-stabilized" reagents. Thirty of the reagents were propagated on primary African green monkey kidney cells, 3 on primary baboon kidney cells, 3 on primary rhesus monkey kidney cells, and the remaining 9 on human amnion cells. Forty-two of the viral antigens were concentrated for use in the production of high-titered specific antisera in large animals.


Susceptibility of salmonellae to cephalosporins and to nine other antimicrobial agents. Three cephalosporin-related antibiotics and nine other antimicrobial agents were studied for in vitro effectiveness against 54 recently isolated strains of Salmonella. Minimal inhibitory concentrations determined by the plate dilution method demonstrated the following percentages of resistance: ampicillin, 6%; tetracycline, 13%; streptomycin, 52%; sulfadiazine, 94%; cephaloglycin, 96%; and lincomycin, 100%. No strains were resistant to cephalothin, cephaloridine, chloramphenicol, colistimethate, kanamycin, and polymyxin B. The commonest serotype studied, S. typhimurium, showed the greatest antibiotic resistance, with 21% resistant to ampicillin, 36% resistant to tetracycline, and 71% resistant to streptomycin. Cephalothin and cephaloridine were highly effective in vitro but inhibitory concentrations of 20 to 40 mug of cephaloglycin per ml were required for the majority of Salmonella strains.


Lecithinase activity of mutant and wild strains of Clostridium perfringens.


Virucidal properties of dimethyl sulfoxide.


The nature of toxins of herpes simplex virus. I. Syncytial giant cell producing components in tissue culture.


A study on the mechanism of polyoma-induced activation of the cellular DNA-synthesizing apparatus. Synchronization by FUdR of virus-induced DNA synthesis.


Drug responses in ganglia perfused with sodium acetate.


Studies on the site of replication of vesicular stomatitis virus.


Studies on rat paw edema induced by S. aureus.


Effect of bretylium tosylate on angiotensin pressor responses in the pithed rat.


Antagonistic effects of beta-adreneric blocking agents on responses to adrenaline in vascular smooth muscle.


Embolic mononeuropathy and bacterial endocarditis.


Regulation of pyruvate carboxylase by coenzyme A and acyl coenzyme A thio esters.


Investigation of the conformation of beta-diphosphopyridine nucleotide (beta-nicotinamide-adenine dinucleotide) and pyridine dinucleotide analogs by proton magnetic resonance.


Glycopeptides from fibrinogen and fibrin.


Photochemical and electron transport reactions of bacterial photosynthesis.


The binding of adenosine triphosphate to myosin.


Induction of phosphopyruvate carboxylase in neonatal rat liver by adenosine 3',5'-cyclic monophosphate.


Phosphohydrolase and phosphotransferase activities of intestinal glucose 6-phosphatase.


Diagnosis of acoustic neurinoma by pneumoencephalo-roulette tomography. Technical note.


Melanoma with a coincidental acoustic neurinoma. Case report.


Susecptibility of ducklings to different strains of Rous sarcoma virus and to other avian tumour viruses.


Propagation of B77 virus and three strains of Rous sarcoma virus in Japanese quail.


Biochemical and histochemical of aerobic oxidative metabolism of oral tissues: VI. Oxidase histochemistry of human gingiva--pre and post gingivectomy.


Diabetes mellitus: induction in mice by encephalomyocarditis virus. Hyperglycemia and lesions of the pancreatic islets of Langerhans developed in some, but not all, adult mice infected with a variant of the encephalomyocarditis virus. Large amounts of virus were recovered from the pancreas during acute stages of infection. At this time blood glucose concentrations were markedly elevated and the islets of Langerhans exhibited focal necrosis and degranulation of beta cells. Evidence of abnormal glucose metabolism persisted for varying periods after recovery from the infection. The islets of Langerhans of chronically hyperglycemic mice were distorted and decreased in size, and the beta cells were degranulated. Encephalomyocarditis virus appears to cause diabetes mellitus by reducing the mass of functional beta cells of the islets of Langerhans.


Herpes simplex infection of the female genital tract. I. Incidence of infection.


Enzymatic degradation and electrophoresis of human angiotensin I.


Are observation wards obsolete? A review of one year's experience in an acute male psychiatric admission unit.


A clinical investigation of phobias.

A clinical investigation of phobias.


The action of antibiotics on heteroglucan production by strains of Azotobacteriaceae.


Isolation and purification of the D(-)beta-hydroxybutyric dehydrogenase of Azotobacter vinelandii.


Mucoepidermoid tumor of lacrimal gland.

Mucoepidermoid tumor of lacrimal gland.


Heterogeneous catalysis of aspirin degradation in chloroformic solution and its relationship to the determination of salicylic acid in buffered aspirin products.


Iron-adjuvant interactions observed with diffuse reflectance spectroscopy.


Quantitative determination of gallamine triethiodide.


An outbreak of swine pox-like disease in Tottori prefecture, Japan.


Hydrogenated pteridine derivatives with high group transfer potential.


Pressor response to infusion of angiotensin into the portal vein of the rat.


Role of leucocidin and triphosphoinositide in the control of potassium permeability.


Isolation of membrane-associated tumour-specific antigens from rat hepatomas induced by aminoazo dye.


Double infections of cultured cells.

Double infections of cultured cells.


Mechanisms of sustained increases of firing rate of neurones in the rat cerebral cortex after polarization: role of protein synthesis.


Vitamins, sugars and ethanol metabolism in man.


Density gradient centrifugation of red blood cells carrying the mammary tumour virus.


Regulation of activity and biosynthesis of enzymes controlling gluconeogenesis and glycolysis.


Response of calves to intravenous and repeated intradermal inoculation of bovine papilloma virus.


Relationship of donor age to adsorption of foot-and-mouth disease virus by mouse tissues.


Isolation of a parainfluenza virus from sentry dogs with upper respiratory disease.


Nerve compression syndrome in derangements of the proximal and distal radial ulnar joints.


Some studies of infectious bovine rhinotracheitis (IBR) virus infection in calves. Six dairy calves, six and one-half to nine months old, were exposed to a strain of infectious bovine rhinotracheitis (IBR) virus of bovine fetal origin by one of the various routes - nasal, vaginal, preputial or contact. Neither after initial exposure nor following challenge of their immunity did any of these animals manifest the IBR respiratory syndrome, although two of them (inoculated per vagina/prepuce) developed pustular vulvovaginitis or balanoposthitis. Also, one five-day old dairy calf which had received colostrum and milk of its IBR-immune dam, was inoculated intranasally with the same strain of IBR virus. This animal exhibited severe signs of IBR. The virus was recovered from all but three of the seven calves after initial exposure and from all but one animal following challenge of their immunity. Immune responses of these calves resembled those of adult cattle.


A virus associated with vulvitis and balanitis in the horse-- preliminary report.


ESR studies of the role of manganese in the process of ascorbic acid oxidation.


Preferential chromosome loss in a Rous rat sarcoma in response to environmental changes.


Synergism of the Schmidt-Ruppin strain of the Rous sarcoma virus and cytidine triphosphate in the induction of chromosome breaks in human cultured leukocytes.


Mechanism of chromosome breakage--a new theory.


Histochemical study of enzyme systems in frozen dried tissue.


Observations on fibroma in poultry.

Observations on fibroma in poultry.


Psychoanalytic theory of the instinctual drives in relation to recent developments.


Structure and amount of positional isomers of monounsaturated fatty acids in human depot fat. Positional isomers of decenoic, dodecenoic, and tetradecenoic acids of human adipose tissue have been separated by gas-liquid chromatography and their amounts determined by oxidative cleavage. The following acids have been shown to be present: 7-decenoic, 8-decenoic, 9-decenoic, 7-, 8-, and 9-dodecenoic, and 7-, 8-, 9-, and 11-tetradecenoic acids. Among all isomers of the even-numbered acids from C(10) to C(18) the cis-9-isomer predominates. With increasing chain length, however, the content of 9-isomer decreases and the number of isomers increases. No 3- or 5-enoic acids could be detected. The origin and biosynthesis of all isomers are discussed.


Granular cell myoblastoma of the vulva.

Granular cell myoblastoma of the vulva.


Adrenocortical activity of the African.

Adrenocortical activity of the African.


Studies o the levels of 17 hydroxy-corticoids in 24-hour specimens of urine from five quadriplegic patients and two paraplegic patients admitted to the Royal North Shore Hospital, Sydney.


Overlapping conformation changes in spin-labeled hemoglobin.


Early effects of 3',5'-adenosine monophosphate on the fluxes of calcium end potassium in the perfused liver of normal and adrenalectomized rats.


Polynucleotide sequence homologies among polioviruses.


The biological activity of 25-hydroxycholecalciferol, a metabolite of vitamin D3.


Inhibition of the anticoagulant activity of neodymium chloride by sodium pyrophosphate.


Nature of the increase in liver microsomal glucose-6-phosphatase activity during the early stage alloxan-induced diabetes.


Sensitivity to angiotensin and adrenocorticotrophic hormone in the sodium deficient rat.


Gonadotrophic regulation of precursor incorporation into ovarian RNA, protein, and acid-soluble fractions. I. Effects of pregnant mare serum gonadotrophin (PMSG), follicle-stimulating hormone (FSH), and luteinizing hormone (LH).


Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40.


Regulation of adrenal growth. Differences in the effects of ACTH in normal and dexamethasone-suppressed guinea pigs.


Membrane glycoprotein biosynthesis: changes in levels of glycosyl transferases in fibroblasts transformed by oncogenic viruses.


Characteristics of REM sleep following different conditioned rates of waking eye movement in the monkey.


Cancer of the male breast. A report of 40 cases.


Migration of radiation damage in DNA.

Migration of radiation damage in DNA.


Cinecystourethrography in patients with spinal cord injuries.


Gas gangrene of the anterior abdominal wall.


Bacterial cytochromes.

Bacterial cytochromes.


The poxviruses.

The poxviruses.


The oncogenic DNA viruses: a review of in vitro transformation studies.


Criteria for assessing effects of drugs on postsynaptic inhibition.


The effect of encephalomyocarditis-virus ribonucleic acid on amino acid incorporation in two mammalian cell-free protein-synthesizing systems.


The solubilization and partial fractionation of the thiol components of isolated nuclei.


Studies on isoniazid-copper interaction.


The mechanism of thyrotrophin action in relation to lipid metabolism in thyroid tissue. 1. The effects of thyrotrophin in vitro on the incorporation of [(14)C]-glucose, -glycerol, -palmitate and -oleate into the lipids of thyroid tissue were examined. 2. Thyrotrophin increased the incorporation of these (14)C-labelled precursors into phosphatidylinositol specifically. 3. Thyrotrophin also increased the proportion of (14)C radioactivity from labelled glucose, glycerol, palmitate and oleate incorporated into the 1,2-diglycerides. 4. The addition of thyrotrophin to thyroid slices for 10min., after 2hr. of prelabelling with [(14)C]glycerol, also increased the proportion of (14)C radioactivity incorporated into the 1,2-diglyceride fraction. 5. After incubation of thyroid tissue with [1-(14)C]palmitate, thyrotrophin caused a two- to three-fold increase in the specific radioactivity of palmitate isolated from phosphatidylinositol and 1,2-diglycerides. In contrast, the specific radioactivity of palmitate isolated from the choline and ethanolamine phosphoglycerides, 1,3-diglycerides and triglycerides was not increased by thyrotrophin.


Enzyme studies on the esterification of vitamin D in rat tissues. 1. The mechanism of vitamin D esterification in the rat was studied with liver, small-intestinal mucosa, pancreatic juice and blood plasma as enzyme sources and [1-(3)H]cholecalciferol, [U-(14)C]ergocalciferol and [4-(14)C]cholesterol as substrates. 2. No esterification of vitamin D could be detected with liver preparations nor with homogenates or acetone-dried powder extracts of intestinal mucosa. 3. Pancreatic juice esterified [1-(3)H]cholecalciferol with oleic acid, and specificity studies indicated that a cholesterol-esterifying enzyme was using vitamin D as substrate. 4. Plasma cholesterol-esterifying enzyme also esterified vitamin D. 5. The specificity of the esterification reaction is discussed in relation to (a) the molecular structure of the substrates and (b) their availability, in a micellar solution, to the enzyme. 6. It is concluded that cholesterol-esterifying enzymes esterify vitamin D in vivo during absorption from the small intestine and while it is transported in blood.


Studies of an established canine kidney-cell line.


Steoid delta-isomerase of the bovine adrenal gland: kinetics, activation by NAD and attempted solubilization.


Sedimentation pattern of soluble protein from thyroids of iodine deficient rats; acute effects of iodide.


Mixed cryoimmunoglobulinaemia in infectious mononucleois and Cytomegalovirus mononucleosis.


Proline as an intermediate in the reductive deamination of ornithine to delta-aminovaleric acid. Fresh extracts of cells of Clostridium botulinum reduced a limited amount of ornithine to delta-aminovaleric acid, but at high substrate concentrations a considerable amount of an amino compound accumulated which was neutral at pH 4.2. Aging of the extracts at -10 C or freezing and thawing resulted in the loss of the ability to produce delta-aminovaleric acid, but the ability to produce the neutral compound was retained. This compound was separated by column chromatography, and was found to be identical to dl-proline with respect to (i) R(F) upon paper chromatography, (ii) migration rates upon paper ionophoresis, (iii) spectrum of the product of the ninhydrin reaction, (iv) oxidation with d-amino acid oxidase, and (v) rate of reduction to delta-aminovaleric acid by cell extracts. The intermediate role of proline in the reduction of ornithine to delta-aminovaleric acid was indicated by (i) rate studies with and without an added electron donor and with and without inhibitors of proline reductase, (ii) the initial accumulation of radioactive proline to the exclusion of radioactive delta-aminovaleric acid from (14)C-l-ornithine in the presence of low levels of carrier proline, and (iii) the initial accumulation of proline at low levels prior to a significant accumulation of delta-aminovaleric acid in reaction mixtures in which the latter compound was the primary product after a longer incubation time. The conversion of ornithine to proline was the rate-limiting step in the presence of a good electron donor (alanine). The mechanism of the conversion of ornithine to proline has not been established. Preliminary data indicated that it may involve an oxidation to glutamic-gamma-semialdehyde and its equilibrium product, Delta(1)-pyrroline-5-carboxylic acid.


Staphylococcal hyaluronate lyase: purification and characterization studies. Staphylococcal hyaluronate lyase (hyaluronidase) derived from a pathogenic strain of staphylococcus was purified by means of salt fractionation with ammonium sulfate and gel filtration through Sephadex G-100. Most of the enzyme activity from concentrated culture supernatant fluids of staphylococci was obtained in a fraction precipitated by 90 to 100% saturation with ammonium sulfate. A small amount of enzyme was also precipitated by 80 to 90% saturation with the salt. The hyaluronidase-rich fractions did not contain other staphylococcal enzymes, such as coagulase, protease, lipase, and staphylokinase. These enzymes were present in the original concentrates. Molecular sieving chromatography of the partially purified enzyme by filtration through Sephadex G-100 resulted in a further increase in specific enzyme activity. However, more than one active peak was obtained after gel filtration, thus suggesting that there may be more than one molecular form of the enzyme. Immunodiffusion in agar gel of the chromatographically purified enzyme fraction, with immune serum from rabbits injected with concentrated staphylococcal culture supernatant fluids, indicated that there was one major antigen. A similar antigen, giving reactions of identity with the purified material, was present in the original culture supernatant fluid.


Tumours and other diseases of the salivary glands in relation to general physiology and pathology.


Site in cell-free protein synthesis sensitive to diphtheria toxin. The effects of diphtheria toxin on cell-free protein synthesis in a bacterial system, and preparations obtained from animals that were sensitive and resistant to toxin were examined. In the presence of nicotinamide adenine dinucleotide (NAD), toxin inhibited the incorporation of amino acids by endogenous and synthetic polynucleotides in both rat liver and guinea pig liver cell-free systems that were exposed to 6 Lf units per ml of toxin. A cell-free system derived from Streptococcus faecalis was resistant to high concentrations of toxin. Dialyzed toxin-antitoxin floccules that are formed in the presence of NAD and the 105,000 x g supernatant fluid from rat liver contain NAD. Such floccules are also active in protein synthesis in the absence of added transferase I or II. An operational model presents the view that the intoxication complex is formed at the ribosomal level and occurs in two steps. First, the toxin molecule binds to transferase II and alters its stereospecific relationship to transferase I, but it does not result in an inactive complex. Second, the stereospecific alteration in transferase I, but it does not result in an inactive complex. Second, the stereospecific alteration in transferase II caused by the binding of diphtheria toxin allows NAD to bridge between transferase I and II, which then results in an inactivated complex. The sensitivity of the cell-free system derived from the normally resistant rat implies that in some cells the cell membrane serves as a permeability barrier to the toxin molecule. The resistance of bacterial cell-free protein synthesizing systems to diphtheria toxin may reflect basic differences between transferase enzymes from bacterial and mammalian sources.


Combined surgical-radiological treatment of parotid gland fistulae.


Radioautographic study of epidermal cell proliferation and migration in normal and neoplastic tissues of rabbits.


Effects of cortisone on interferon response of germfree mice to mouse hepatitis virus.


Interferon response and age-related resistance of germfree mice to mouse hepatitis virus.


Paraganglioma (chemodectoma) of the middle ear and mastoid.


Morphological conversion, hyperconversion and reversion of mammalian cells treated in vitro with N-nitrosomethylurea.


Assessing skeletal maturity: comparison of the atlas and individual bone technics.


Cytochrome as a guide to classifying bacteria: taxonomy of Microbacterium thermosphactum.


Production of self-directed antibody by tumour cells.


Vinca alkaloid neuropathy: nerve biopsy studies in rats and in man.


Glucose metabolism in five neuromuscular disorders.


Herpetic keratoconjunctivitis: therapy with synthetic double-stranded RNA. A study was undertaken in rabbits to determine how late in the course of keratoconjunctivitis caused by herpes simplex recovery could be effected by an inducer of interferon. Interferon was induced by means of synthetic double-stranded RNA copolymer formed with polynosinic acid: polycytidilic acid RNA. Therapy promotes recovery from severe and fully established keratoconjunctivitis for which treatment was begun as late as 3 days after virus inoculation. No drug toxicity was observed in the therapeutic dose range. These findings further support the proposed role of the interferon mechanism in the natural recovery of already established viral infection. They also suggest the usefulness of interferon inducers in viral infections of man.


Chromatographic separation and photometric analysis of the components of Nile blue sulphate.


Arrhenoblastoma in an Indian Desi hen.

Arrhenoblastoma in an Indian Desi hen.


Studies on acute Marek's disease. I. Characteristics of isolate GA in chickens.


Survival of Marek's disease agent in litter and droppings.


Surface properties of acidic phospholipids: interaction of monolayers and hydrated liquid crystals with uni- and bi-valent metal ions.


Isolation of a membrane fraction enriched in nerve-end membranes from rat brain by zonal cetrifugation.


Human liver glucose-6-phosphatase: characteristics of the detergent-solubilized enzyme.


The reaction between NAD+ and rabbit-muscle glyceraldehydephosphate dehydrogenase.


Serological survey of human arbovirus infections in Southeastern British Columbia.


Self-induced waking: changes in dreams and sleep patterns.


Current trends in group therapy with children.


The interconnexions of the muscle layers of the human colon.


Hand, foot and mouth disease in South Australia.


Inhibitor of antibiotics in bacteriologic agar.


A new overlay for plaquing animal viruses.


Age difference in the intestinal phosphomonoesterase activity of mice.


Inhibition of adrenal steroidogenesis by glutethimide.


Fetal and maternal responses to in utero angiotensin infusions in Macaca mulatta.


Prevention of viral and transplant tumors in hamsters employing killed and fragmented homologous tumor cell vaccines.


Study of forced circus movements.

Study of forced circus movements.


NAD-ase activity and glycolysis in Ehrlich-Lettre carcinoma and liver cell particulates.


Hydrolysis of higher fatty acid esters of p-nitrophenol by rat liver and kidney lysosomes.


Interstitial pulmonary fibrosis, atypical epithelial changes and bronchiolar cell carcinoma following busulfan therapy.


Carcinoma of the pancreas. Results of treatment.


New method for isolating nicotinamide-adenine dinucleotide (NAD) from baker's yeast.


Immune paralysis of thymus cells by bovine serum albumin.


Photometric demonstration of aggregation of slime mould cells showing effects of temperature and ionic strength.


Habituation in relation to state in the human neonate.


The cryptophthalmos-syndactyly syndrome.


Malignant mesodermal mixed tumor (mesenchymoma) of the orbit.


Treatment of nephoroblastoma (Wilms' tumour).


The oxidized forms of cytochrome oxidase.


Retailing of barbecued chickens--a Canadian survey.


Effect of some mutagenic virogens and carcinogens on deoxyribonucleic acid.


Proteins in bioelectricity: the control of ion movements across excitable membranes.


Enzyme secretion in mouse pancreas mediated by adenosine-3'5'-cyclic phosphate and inhibited by adenosine-3'-phosphate.


Spin-labeled mitochondrial lipids in Neurospora crassa.


Changes in fluorescence, turbidity, and birefringence associated with nerve excitation.


Composition and structure of urinary stone.


Adenovirus type 3 epidemic associated with intermittent chlorination of a swimming pool.


Fibrosis in coal workers.

Fibrosis in coal workers.


Quartz content in fibrosis.

Quartz content in fibrosis.


Pontine relay from cerebral cortex to cerebellar cortex and nucleus interpositus.


Direct inhibitory innervation of teleost oculomotor neurons by cerebellar Purkinje cells.


Excitability changes in dendrites of thalamic neurons during prolonged synaptic activation.


Synaptic action of specific visual inpulses upon cat's parastriate cortex.


EMG analysis of myasthenic ACTH deterioration.


The nature of tetraplegia in infectious tetanus.


Analysis of spontaneous subthreshold activity in spinal motoneurons of the cat.


Action of tetrodotoxin on spinal motoneurons of the cat.


Behavioral effects of electrical stimulation of group I muscle afferents in acute thalamic cats.


Contrasting effects of two identified amygdaloid efferent pathways on single hypothalamic neurons.


Receptive fields of single optic nerve fibers in a mammal with an all-cone retina. II: directionally selective units.


Hypernatremia due to hypodipsia and elevated threshold for vasopressin release. Effects of treatment with hydrochlorothiazide, chlorpropamide and tolbutamide.


BHK 21 cell cultures for the large-scale production of foot-and-mouth disease virus.


Latent ECSO virus infections of sucking pigs. I. Isolation of latent enteroviruses from tissue cultures, obtained from kidneys of apparently healthy sucking pigs.


Epizootiological aspects of the presence of haeminhibiting (HI) antibodies agianst viruses influenza A10equine (Prague) 56 and Parainfluenza 3(PI 3) in horse sera in Croatia.


Aglossia-adactylia syndrome.

Aglossia-adactylia syndrome.


Wilms' tumor, congenital heart defects, pheochromocytomas and thyroid cancer.


Effect of adrenergic agents on toad bladder response to ADH, 3',5'-AMP, and theophylline.


Cytomegalovirus mononucleosis.

Cytomegalovirus mononucleosis.


Plasma cell and lymphocytic reaction in the Syrian hamster to transplanted homologous tumor cells transformed in vitro "spontaneously" or by SV 40.


Neoplastic transformation induced by simian virus 40 in Syrian hamster neuroglial and meningeal cell cultures.


Studies of the resistance and susceptibility to viral superinfection of adenovirus 12 hamster tumor cells.


Polyoma virus infection in neonatally thymectomized hamsters.


The weeping mother, an unusual source of variola virus.


Some behavioral studies of simian virus 40 (SV 40).


A virus disease of captive vervet monkeys (Cercopithecus aethiops) caused by a new herpesvirus.


Search for a human leukemia virus.

Search for a human leukemia virus.


Intrinsic interference: a unique interference system used in assaying non-cytopathic viruses.


The search for human hepatitis viruses.

The search for human hepatitis viruses.


Studies on human adenoviruses as inducers of interferon in chick cells.


Plaque variants by Sindbis virus.

Plaque variants by Sindbis virus.


The adsorption and elution of parainfluenza 2 virus.


Infusion of liver tumours.

Infusion of liver tumours.


The inhibition of mammalian mitochondrial NADH oxidation by chloramphenicol and its isomers and analogues.


Two deoxyribonucleases from Novifoff ascites hepatoma cells.


Nucleotide clusters in deoxyribonucleic acids. I. Isolation of purine oligonucleotides.


Reversal of mitochondrial swelling associated with fatty acid oxidation. II. Effects of cytochrome c and carnitine on contraction of fatty acid swollen mitochondria.


The proteins of rapeseed (Brassica napus L.) soluble in salt solutions.


Studies on the chemical constitution of cell-wall lipopolysaccharides from Neisseria perflava.


Regulatory interactions of dehydroepiandrosterone and its sulfate in rat testis tissue.


Gluconeogenesis in rats during cold acclimation.


Body motility during sleep and its relation to the K-complex.


Changes in phosphatase enzymes following denervation of the vallate papilla of the rat.


Emetine on adrenergic transmission.

Emetine on adrenergic transmission.


An investigation of the mechanifm of the luminescent peroxidation of luminol by stopped flow techniques.


Studies on cytochrome c peroxidase. XII. Crystalline synthetic enzymes containing unnatural heme prosthetic groups.


Inosinic acid dehydrogenase of sarcoma 180 cells.


Studies on mutarotases. II. Investigations of possible rate-limiting anomerizations in glucose metabolism.


The occurrence of diphosphoinositide and triphosphoinositide in Saccharomyces cerevisiae.


The purification and properties of rat liver nicotinamide adenine dinucleotide kinase.


The occurrence of autoacceleration during hydrolysis of nucleic acids by micrococcal nuclease.


Purification and properties of deoxyribonucleic acid polymerase from rat liver mitochondria.


Functional consequences of modification of kidney fructose 1,6-diphosphatase by pyridoxal 5'-phosphate.


Slow synaptic responses and excitability in sympathetic ganglia of the bullfrog.


Intracellular analysis of slow inhibitors and excitatory postsynaptic potentials in sympathetic ganglia of the frog.


Effect of atropine on the latency of cochlear potentials.


"Trophic" influences of nerve on muscle.


Specificity of ribonuclease Ch from Chalaropsis species. A ribonuclease is produced by the fungus Chalaropsis sp. that appears to have an absolute specificity for 3'-guanylic acid residues in ribonucleic acid.


Testosterone production by rabbit testis slices.


Corticotrophin treatment in multiple sclerosis.


Mast cells in INH-induced neuropathy of the rat.


Effect of muscular exertion on the reaction of the pituitary-adrenocortical axis in trained and untrained rats.


Influence of corticosteroids on the hypothalamic control of sciatic-evoked potentials in the brain stem reticular formation and the hypothalamus in the rat.


Ethanol-induced hypoglycemia.

Ethanol-induced hypoglycemia.


Reflux pancreatography. An evalauation of contrast agents for studying the pancreas.


Peritoneal extravasation of oily contrast medium following lymphography.


Liver scans in children: abdominal masses simulating metastatic disease.


Submaxillary sialography in combination with pneumoradiography and tomography.


Aldosterone production after suppression of corticotropic secretory activity.


Sleep and birbiturates: some experiments and observations. To determine the effect of barbiturates on sleep two subjects, after a control period, received 200 mg. of sodium amylobarbitone for 26 nights. All night sleep records taken during this period showed that the barbiturate shortened the delay to sleep, increased the total sleep period, lengthened the delay to rapid eye movement (R.E.M.) sleep, and depressed R.E.M. sleep. After five nights R.E.M. sleep returned to baseline values -that is, showed tolerance. On stopping the drug withdrawal phenomena were seen, even to this small dose of the drug.In a second experiment a subject dependent on 600 mg. of Tuinal was found to have low normal R.E.M. sleep while on drugs. On withdrawal, delay to sleep increased and total sleep time fell. R.E.M. sleep was doubled and the delay to R.E.M. became abnormally short.These findings suggest that hypnotics allow sleep to be "borrowed," and that patients should be supported while they are being withdrawn.


Peripheral neuropathy in metachromatich leucodystrophy.


Treatment of vitiligo.

Treatment of vitiligo.


Synovial sarcoma in Indians. (Observations on 22 cases with an emphasis on morbid anatomy).


Immunological studies with human breast tumours.


Comparative metabolic studies on the ovaries of the mice susceptible and resistant to breast cancer.


Hepatoblastoma of the liver. (Report of a case).


The relative sensitivity of various reagents for the detection and differentiation of sugars and sugar derivatives in glycoproteins.


Chromosome pulverization in chinese hamster cells induced by Sendai virus.


Cellular phase of chromosome pulverization induced by Sendai virus.


Experimental aerosol transmission of Yaba virus in monkeys.


Immunologic response of monkeys to aerosols of Yaba virus.


The role of potassium and calcium ions in the effect of epinephrine on cardiac cyclic adenosine 3',5'-monophosphate, phosphorylase kinase, and phosphorylase.


Functional classification of glioblastoma.


Reversible inhibition of the reproduction of DNA viruses by glycosides of 2-phenethyl alcohol and related compounds.


The angiotensin I converting enzyme activity of subtilisin BPN' (Nagarse), a crystalline enzyme from Bacillus subtilis.


A new method of phallography (PhG).

A new method of phallography (PhG).


Cystosarcoma phylloides.

Cystosarcoma phylloides.


Cancer of the prostate:estrogen therapy: natural conjugated estrogen vs. Stillbestrol: A point of view.


Effect of poxvirus infection on host cell deoxyribonucleic acid synthesis. Deoxyribonucleic acid (DNA) synthesis was studied in poxvirus-infected cells by measuring (14)C-thymidine incorporation into viral and host cell DNA. A complete separation of the two species of DNA was achieved by combining the previously used "Dounce method" with a separation method based on different reannealing properties of viral and vertebrate DNA. Shortly after infection of HeLa cells with poxviruses, a burst of viral DNA synthesis occurred in the cytoplasm, but a rapid inhibition of host-cell DNA synthesis in the nucleus was observed. This inhibition of cellular DNA synthesis was also found if an accumulation of viral DNA was prevented. At high multiplicites, ultraviolet-irradiated virus inhibited host-cell DNA synthesis to the same extent as fully infectious poxvirus. Under the same conditions, heating at 60 C for 15 min caused a decrease in the ability of cowpox virus to inhibit host-cell DNA synthesis, but did not produce the same effect on vaccinia virus strain WR.


Rescue of temperature-sensitive poliovirus. A temperature-sensitive strain of type 1 poliovirus, LSc, was functionally rescued when infected cells were incubated at 40 C in the presence of Mahoney, a temperature-resistant strain of type 1 poliovirus. The rescue value was 9% of the mutant yield obtained under permissive conditions. Rescued virus underwent replication, because the progeny of (32)P-labeled LSc were not radiosensitive. Serum inactivation studies with Mahoney specific antiserum indicated that a small amount of phenotypic mixing occurred among the rescued particles. The temperature-sensitive event occurred between 2 and 4 hr postinfection in the developmental cycle of LSc. Neither viral polymerase activity nor virus-induced ribonucleic acid could be demonstrated in infected cells between 2 and 4 hr after infection at 40 C with the temperature-sensitive mutant.


Biosynthesis of host and viral deoxyribonucleic acid during hyperplastic fowlpox infection in vivo. The synthesis of deoxyribonucleic acid (DNA) during in vivo infection of chick epithelium with fowlpox virus was examined by incorporation of tritiated thymidine into the acid-insoluble fraction. The proportion of precursor incorporated into host and viral DNA at various times after infection was determined by chromatography on columns of methylated albumin-kieselguhr. The first 60-hr period of infection was characterized by the synthesis of predominantly host DNA, the rate of production of which increased markedly over the control between 36 and 48 hr postinoculation (PI). Although the replication of viral DNA began between 12 and 24 hr PI, the rate of synthesis was very low during the first 60 hr. In contrast, an abrupt increase in the rate of viral DNA synthesis occurred between 60 and 72 hr PI, concomitantly with a sharp decline of host DNA synthesis. Subsequently, between 72 and 96 hr, the ratio of synthesis of viral DNA to host DNA progressively increased to a maximum of greater than 2:1. The temporal relationship of this biphasic pattern of host and viral DNA synthesis to hyperplasia and viral replication is discussed.


Specific role of each human leukocyte type in viral infections. II. Phytohemagglutinin-treated lymphocytes as host cells for vesicular stomatitis virus replication in vitro. The mitogenic agent, phytohemagglutinin (PHA), added to human mixed leukocyte cultures and to lymphocyte cultures converted small lymphocytes into lymphoblasts and increased lymphocyte susceptibility to vesicular stomatitis virus (VSV). Maximum virus yields were 30- to 1,000-fold higher in PHA-treated than in control cultures. VSV replicated to peak titers before lymphocytes were morphologically transformed by PHA, and virus titers fell as lymphoblast destruction began. PHA neither induced significant VSV replication in polymorphonuclear leukocyte cultures, nor increased the large virus yields in monocyte cultures. The treatment of PHA with heat, digestive enzymes, rabbit anti-PHA serum and serial dilutions failed to dissociate that portion of the PHA extract responsible for the conversion of lymphocytes into virus-susceptible cells from those components responsible for leukoagglutination or lymphocyte transformation.


Role of adenovirus structural proteins in the cessation of host-cell biosynthetic functions. Two of the adenovirus capsid proteins, the fiber and the hexon, complexed with either KB cell or type 5 adenovirus deoxyribonucleic acid (DNA). Maximal binding occurred at 0.01 m NaCl; increasing the ionic strength of the reaction mixture to 0.2 m NaCl resulted in a decrease in the association of either antigen to DNA. Variations of pH between 6.3 and 8.4 did not affect the binding of fiber antigen to DNA. Below pH 7.5, however, there was a small decrease in the ability of the hexon to bind nucleic acid. The association between the adenovirus structural proteins and DNA was reversible and was independent of whether the DNA was native or denatured. The fiber or hexon protein inhibited the DNA-dependent ribonucleic acid (RNA) polymerase and the DNA polymerase from KB cells. On a weight basis, the fiber protein inhibited enzymatic activity to a greater extent than the hexon. Increasing the template DNA concentration decreased this inhibition. The inhibition of the DNA-dependent RNA polymerase activity by either antigen could be reversed by increasing the ionic strength of the reaction mixture. After infection of KB cells with type 5 adenovirus, the levels of DNA and RNA polymerases remained unchanged for 15 to 20 hr. Thereafter, the specific activity of both enzymes decreased. By 30 hr postinfection, the polymerase activities were only about 30% of the enzyme activities in uninfected cells.


Hybrid of monkey and human adenoviruses. Following joint replication of monkey SA7 adenovirus (C8 strain) and human adenovirus type 2 in green monkey kidney tissue culture, a virus possessing the properties of a hybrid was obtained. It was designated Ad2C8. Ad2C8 preparations contained two types of viral particles: human adenovirus type 2, and hybrid particles. The hybrid virions multiplied in green monkey kidney cells in the presence of human adenovirus types 1, 2, and 3, but not 3 and 7, and acquired the capsid of the helper adenovirus. The hybrid can serve as a helper for human adenoviruses. It can apparently induce T antigen of the C8 virus but, in contrast to the latter, does not induce tumors in hamsters.


Electron microscopy of herpes simplex virus. I. Entry. Although capsids of herpes simplex virus were encountered within phagocytic vesicles, they were more commonly observed free within the cytoplasm. Stages in the release of virus from vesicles were not seen. There appeared to be five distinct steps in the process whereby the virus initiates infection: attachment, digestion of the viral envelope, digestion of the cell wall, passage of the capsid directly into the cytoplasm, and digestion of the capsid with release of the core. Antibody probably interferes with the first two stages.


Replication of defective simian virus 40 and a monkey cell-adapting component in human kidney cells.


Unilaateral proptosis secondary to intracerebral glioblastoma.


Electron microscopy of herpes simplex virus. II. Sequence of development. Examination of infected cells at sequential intervals after infection revealed that the first viral forms to appear were capsids enclosing cores of low density. Not until the 6th hr were dense cores encountered, and at approximately the same time enveloped virus was seen. Envelopment occurred most frequently in close proximity to the nuclear surface, although the process was also encountered within the nuclear matrix and in the cytoplasm. There was often extensive proliferation of the nuclear membrane. Envelopment of the virus by budding from the cell surface was not observed. It was concluded that enveloped virus consitutes the infectious particle and that the unenveloped capsid is unstable outside the cell. Nevertheless, it is likely that capsids enclosing infectious nucleic acid can pass directly from one cell to another after fusion has taken place.


Cytosarcoma phyllodes mammae.

Cytosarcoma phyllodes mammae.


Studies on the fractionation of urinary 11-deoxy-17-ketosteroids with gas-liquid chromatography, with special reference to the evaluation of determination procedures and results in aged subjects and in several pathological conditions.


The inactivation of foot-and mouth disease virus by chemicals and disinfectants.


A controlled study of all-night sleep in overactive imbecile children.


Development of pediatric cardiology.

Development of pediatric cardiology.


Distribution of ACTH in the hypophysis of Necturus.


Statistical analysis of membrane potential fluctuations. Relation with presynaptic spike train. In a study of integration at the single neuron level, the relationships between the postsynaptic membrane potential and the presynaptic spike train were analyzed. Fluctuations in membrane potential of neurons in the visceral ganglion of Aplysia were measured and described by histograms. The histogram estimates the probability density function of the membrane potential. Comparisons were made among histograms when there was no synaptic input, and when there was a single input in which variations were made in the PSP (postsynaptic potential) sign, i.e. excitatory or inhibitory, and arrival statistics, e.g. slow or fast, regular, Poisson-like, or patterned. This was examined in cells where the membrane potential was constant and in cells in which there was spontaneous pacemaker activity. The form of the histogram depended on whether the neuron was spontaneously quiescent or a pacemaker, or whether it received presynaptic input and, if it did, on the sign and temporal characteristics of such input. From such histograms the mean firing rate of output spike trains can be predicted; additional information of a temporal nature is required, however, to predict features of the interval structure of the output train. Suggestions are made concerning the way the nervous system might utilize the information summarized in the membrane potential histogram.


How can the voluntary bodies best assist in the global attack on tuberculosis. A panel discussion held in Paris on September 20th and 21st 1962.


Two unusual cases of segmental colitis. Fibroplastic linitis of the colon due to gastric carcinoma and regional colitis with retroperitoneal fibrosis.


Effects of ACTH and cortisol on water movement in isolated intestine of the eel, Anguilla japonica.


Effect of adenine and nicotinamide on amino acid incorporation of cell-free systems inhibited by diphtheria toxin and NAD.


Post menopausal bleeding related to estrogen therapy. An increasing problem.


Effect of the number of primed cells on the production of IgM and IgG antibodies in an adoptive secondary response.


Effect of reticuloendothelial system stimulation on resistance to Rous sarcoma virus infection in chicks.


Mast cells in the nervous system.

Mast cells in the nervous system.


Cutaneous and penile bovine fibropapillomatosis and its control.


Interferon response of human leukocytes during intrauterine and postnatal life.


Cytomegaloviremia following congenital infection.


Screening of newborn infants for cytomegalovirus infection.


Radiologic case presentation no. 4.

Radiologic case presentation no. 4.


Demyelinating disease in a woman from tropical South America with features of multiple sclerosis and neuromyelitis optica. Clinical, protein chemistry and pathological findings.


Impaired secretion of epinephrine in response to insulin among hypophysectomized dogs.


The normal and abnormal pancreatic scan.


Epidermolysis bullosa and amyloidosis: a case report.


ACTH drip in prednisone resistant pemphigus.


Experimental diphtheritic neuropathy. A pathological and electrophysiological study.


The pathology of the muscle spindle. A study of biopsy material in various muscular and neuromuscular diseases.


Rifampin and ethambutol in the retreatment of advanced pulmonary tuberculosis.


A continuing study of patients with "open negative" status at Battey State Hospital.


Long-term dwarfing effects of corticosteroid treatment for childhood nephrosis.


Citramalate lyase of Clostridium tetanomorphum.


Vascular reactivity studies in hypertension.


The effects of alpha-adrenergic receptor blockade and sympathetic denervation of the pressor action of angiotensin in man.


Interactions between angiotensin, noradrenaline and serotonin on the peripheral blood vessels in man.


Ferredoxin dependent synthesis of alpha-ketoglutarate and pyruvate by extracts of the green photosynthetis bacterium Chloropseudoonas ethylicum.


Effect of hydroxyurea on ribonucleotide reductase.


Effects of the oxidation state, ligands, detergents and aging on the conformation of cytochrome oxidase.


Effect of 5-fluorouracil and 5-bromo-2'-deoxyuridine on the induction of thymidine kinase in polyoma virus-infected mouse embryo cells.


Disaccharidase activity of renal tissue.


Iliacus haematoma syndrome as a complication of anticoagulant therapy.


Rapid diagnosis of herpesvirus hominis infections in superficial lesions by immunofluorescent antibody technics. Scrapings from lesions of the skin and mucous membranes of 14 patients suspected of suffering from suspected Herpesvirus hominis infections were examined by both fluorescent antibody and routine isolation techniques. There was complete correlation between results by both methods in all 14 cases, 12 being positive. No positive fluorescence was obtained from scrapings of seven control patients with a variety of skin diseases. Thirteen patients with corneal lesions were similarly investigated. Of the 10 scrapings which showed positive fluorescence, nine were confirmed by virus isolation. It is suggested that as more antiviral agents become available the application of a fluorescent antibody technique for testing virus sensitivity in tissue culture could become a practical method.


Determination of 3',5'-adenosine monophosphate with a method based on a radioactive phosphate exchange reaction.


Studies on the respiratory chain-linked reduced nicotinamide adenine dinucleotide dehydrogenase. XV. Interactions of piericidin with the mitochondrial respiratory chain.


Isolation and characterization of beta-methylene-l-(+)-norvaline from Lactarius helvus.


Studies on heart phosphofructokinase. Thiol groups and their relationship to activity.


The enzymatic carboxylation of phosphoenolpyruvate. IV. The binding of manganese and substrates by phosphoenolpyruvate carbosy-kinase and phosphoenolypyruvate carboxylase.


Cytochrome P-450 involvement in the oxidation of n-octane b cell-free extracts of Corynebacterium sp. strain 7E1C.


Characteristics of microbial diphosphopyridine nucleotidases containing exceptionally large amounts of polysaccharides.


Diagnostic value of liver function tests and Entamoeba histolytica antibody studies in East Africans. Liver function tests and Entamoeba histolytica (E.H.) antibody titres have been studied in a variety of tropical and liver diseases. Patients with hepatic amoebiasis had a raised E.H. antibody titre and tended to have a low pseudocholinesterase activity. In two cases of amoebic liver abscess, which were treated by aspiration and emetine, there was a remarkable drop in the E.H. antibody titre. In a number of other hepatic and tropical conditions the E.H. antibody titre was not significantly raised and the liver function tests were typical of the various conditions studied.


Rgulation of intestinal alkaline phosphatase levels in the rat. Role of the adrenal cortex. Bilateral adrenalectomy produces a reduction in the alkaline phosphatase concentration in rat intestine, which is not prevented by the administration of saline, but is prevented by the administration of cortisone. The administration of A.C.T.H. to normal rats leads to a rise in intestinal alkaline phosphatase levels associated with a small increase in the weight of the adrenals, while the administration of hydrocortisone produces large increases in the enzyme, even with doses which cause significant hypoplasia of the adrenal glands. The significance of these findings in relation to mechanisms of fat absorption and the treatment of steatorrhoea is discussed.


Serologic relationship o a simian Herpes virus (SA8) and Herpes simplex virus: heterogeneity in the degree of reciprocal cross-reactivity shown by rabbit 7S and 19S antibodies.


Granular cell myoblastoma of the bladder: report of a case.


Carcinoma of the urachus: Report of two cases and review of the literature.


Enlarged jugular foramen.

Enlarged jugular foramen.


Effect of connective tissue and collagen on platelet lactate production: role of acid mucopolysaccharides.


Studies on the physico-chemical inactivation of rabbit inerferons.


Histological effects of procedural and environmental factors on isolated rat heart preparations.


Attempts to infect rhesus monkeys with human type 4 adenovirus.


Virus carrier cells and virus-free cells in fetal rubella.


The mammary tumor virus in organs of mice: normal distribution and postinoculation localization.


Kinetics of the development of factors responsible for inerferon-induced resistance.


Antithyroid effects of 3-amino-1,2,4-triazole.


Yaba tumor virus. I. Studies on pathogenesis and immunity.


Radiation responeses of embryonal and SV40 transformed hamster cells in culture.


Method for increasing the diagnostic capacity of the complement fixation test in some resporatory virus infections.


Increased susceptibility to murine hepatitis virus infection by treatment with iron salts.


The spectrum of rhinovirus inhibitor by 2-(alpha-hydroxybenzyl)-benzimidazole and D-(-)-2-(alpha-hydroxybenzyl)-benzimidazole HC1.


Recovery of virus from bulls affected with foot-and-mouth disease.


Clearing-factor lipase in adipose tissue. A possible role of adenosine 3',5'-(cyclic)-monophosphate in the regulation of its activity. 1. The rise in clearing-factor lipase activity that occurs when epididymal fat bodies from starved rats are incubated in appropriate media in vitro is inhibited in the presence of 6-N-2'-O-dibutyryl-3',5'-(cyclic)-AMP (1mm). 2. Inhibition occurs at a concentration of glucose in the incubation medium of 1.3mg./ml. or less, but not at a glucose concentration of 2.4mg./ml., unless caffeine (1mm), an inhibitor of 3',5'-(cyclic)-nucleotide phosphodiesterase, is also present. Caffeine (5mm) alone inhibits the rise in clearing-factor lipase activity at a glucose concentration of 2.4mg./ml. of medium. 3. The concentration of free fatty acids in the epididymal fat bodies normally falls during incubations in vitro as the rise in clearing-factor lipase activity occurs. In the presence of 1mm-6-N-2'-O-dibutyryl-3',5'-(cyclic)-AMP, however, either the tissue free fatty acid concentration is increased or it does not fall to the same extent. The concentration of glucose in the incubation medium is important in determining the direction and extent of the changes in tissue free fatty acid concentration that occur in the presence of 6-N-2'-O-dibutyryl-3',5'-(cyclic)-AMP. 4. Free fatty acid concentrations in epididymal fat bodies in vivo rise as the clearing-factor lipase activity of the tissue falls during starvation. 5. The possibility that the concentration of 3',5'-(cyclic)-AMP in adipose tissue may regulate clearing-factor lipase activity, and that the regulation may occur through effects of the nucleotide on tissue free fatty acid concentrations, is discussed.


The displacement of calcium ions from phospholipid monolayers by pharmacologically active and other organic bases. 1. The binding of (45)Ca(2+) to a monolayer of phosphatidylinositol at the air-water interface was maximal when the separation of the phospholipid head groups approximated to the diameter of a hydrated Ca(2+) ion. 2. The displacement of Ca(2+) adsorbed on monomolecular films of phosphatidylinositol by a series of drugs (both narcotic and excitatory) and other organic bases was related to the ability of the bases to penetrate into the film. 3. With films of phosphatidylinositol at constant area, and at an initial surface pressure of 10dynes/cm., the displacement of Ca(2+) by increasing concentrations of the local anaesthetic, tetracaine, was linearly related to the change in surface pressure (Deltapi) caused by the penetration of the drug. 4. Deltapi and the displacement of Ca(2+) showed a related fall when the initial surface pressure of the phosphatidylinositol film was increased from 4 to 40dynes/cm. both at a constant bulk tetracaine concentration and when this latter concentration was adjusted to keep it at a constant ratio to the surface density of phosphatidylinositol molecules. 5. The displacement of Ca(2+) from phosphatidylinositol films by cetyltri-methylammonium ions was directly compared with the surface concentration of the base in the film, measured by using labelled base and a surface-radioactivity technique. 6. The ability of a series of straight-chain aliphatic amines to displace Ca(2+) from phosphatidylinositol films increased with the number of carbon atoms up to C(12). However, there was a marked jump in the displacing activity after hexylamine, and this could probably be correlated with the carbon chain's being of sufficient length to just reach the hydrophobic fatty acid chains of the orientated phospholipid molecules with the charges on both substances in juxtaposition.


Changes in the concentrations of some phosphorylated intermediates and stimulation of glycolysis in liver slices. 1. The concentrations of some phosphorylated glycolytic intermediates and of NADH were measured in glycolysing rat liver slices. 2. In anaerobically incubated liver slices the concentration of hexose monophosphates decreases during the first 20min. of incubation, whereas the concentrations of fructose diphosphate and triose phosphates increase progressively. 3. In liver slices from fed rats, previously exposed to oxygen, the stimulated anaerobic glycolysis is accompanied by an increase in the concentration of hexose monophosphates; fructose diphosphate and triose phosphates maintain the concentrations reached at the end of the aerobic preincubation. 4. The same pattern in the concentration of glycolytic phosphorylated intermediates is seen under all conditions where aerobic preincubation brings about a stimulation of anaerobic glycolysis. A similar pattern is also found in liver slices from fed rats incubated anaerobically in the presence of fructose; these slices display a high glycolytic activity, which is not further affected by previous aerobic incubation. 5. The concentration of NADH decreases in liver slices during exposure to oxygen; during the subsequent anaerobic glycolysis the concentration increases but is always lower in preincubated than in non-preincubated liver slices. 6. The results of the present experiments suggest that the limiting step mainly affected by the preliminary exposure to oxygen might be at the level of the utilization of triose phosphates.


Leaf extract purification by silver nitrate-silica gel thin-layer chromatography.


Maintenance of axonal conduction and membrane permeability in presence of extensive phospholipid splitting.


Induction of collagenolytic and proteolytic activities by anti-inflammatory drugs in the skin and fibroblast.


Properties of hemoglobin M. Unequivalent nature of the alpha and beta subunits in the hemoglobin molecule.


Regional and subcellular distribution of adenyl cyclase and 3',5'-cyclic nucleotide phosphodiesterase in brain and pineal gland.


Aniridia and wilms's tumour (nephroblastoma).


Isolation and amino acid composition of insoluble elastin. Bovine foetal and adult aorta and ligamentum nuchae.


Characterization of four transplantable mammotropic pituitary tumor variants in the rat.


Some ultrastructural features of Yoshida ascites hepatoma.


Effects of divalent cations on K+ transport in rat liver and Morris hepatoma 5123tc.


Comparative incorporation of tritiated thymidine and cytidine into the mitochondrial and nuclear DNA and RNA of two transplantable hepatomas (3924a and h-35tc2) and host livers.


Micro-determination of human plasma renin activity with the addition of homologous substrate.


Cystathioninuria in hepatoblastoma.

Cystathioninuria in hepatoblastoma.


Interference of pyrophosphate in the determination of orthophosphate.


Abnormal urinary excretion of p-hydroxyphenyllactic acid in Wilms' tumor.


X-radiation from positive ion beams incident on insulator targets.


Innervation of the vesical detrusor muscle by the ganglia of the pelvic plexus.


Enzyme histochemistry of the choroid plexus in rat and squirrel monkey.


Human urinary polypeptides with parathyroid-like activity.


Stimulation and inhibition of ACTH release in patients with pituitary disease.


Serum renin activity during normal pregnancy: effect of alterations of posture and sodium intake.


Effect of iodized oil on serum TSH determined by immunoassay in endemic goiter subjects.


Measurement of plasma angiotensin II and correlation with renin activity.


Automated enzymatic fluorometric method for the determination of pyruvic and lactic acids in blood. A simple automated fluorometric method for the estimation of lactate and pyruvate is described. Analyses can be performed at 60/hour and can conveniently determine pyruvate and lactate in the ranges 0-5 mg. and 0-30 mg/100 ml. The methods give a high degree of analytical precision and good yields from recovery experiments.


Calfhood viral diseases and early vaccination studies.


Thyroid carcinoma and Cushing's syndrome. A report of two cases with a review of the common features of the "non-endocrine" tumours associated with Cushing's syndrome. Two cases of thyroid carcinoma and Cushing's syndrome are reported. Nine other previously published cases of this association are reviewed: in one the thyroid tumour was described as medullary, in two as papillary, and in the other six as anaplastic, undifferentiated, atypical, or solid carcinoma. Both of our own cases were medullary carcinomas of the thyroid, and on reviewing the histology of five of the other cases all proved to be medullary carcinoma with identifiable amyloid in the stroma. A consideration of the temporal relationships of the development of the carcinoma and of Cushing's syndrome suggested that in the two cases with papillary carcinoma these conditions could have been unrelated, but that in eight of the nine cases with medullary carcinoma there was evidence that thyroid carcinoma was present at the time of diagnosis of Cushing's syndrome. The other main groups of the so-called ;non-endocrine' tumours associated with Cushing's syndrome are briefly reviewed, and evidence that a surprising number of these cases are related to carcinoid tumours is put forward. Medullary carcinoma of the thyroid is also probably related to this group of tumours. It is suggested that the great majority of the tumours associated with Cushing's syndrome are derived from cells of foregut origin which are endocrine in nature. In neoplasms derived from these cells the polypeptide hormone may well be imperfectly formed, and possess an amino-acid sequence in common with ACTH or other biologically active polypeptides.


Mental retardation and dermatoglyphics in a family with the oral-facial-digital syndrome.


The cation-sensitive phosphatases of the leucocyte cell membrane.


Organizational difference of cell surface "hematoside" in normal and virally transformed cells.


Interaction of homologous transfer RNA with yeast aminoacyl-RNA synthetases.


Glutamate dehydrogenase in nuclear and mitochondrial fractions of rat liver.


Selective desensitization of the allosteric glutamic dehydrogenase from Blastocladiella by Hg.


Ornithine activating enzyme from Bacillus brevis.


The role of calcium (Ca++) in TSH and dibutyryl 3'5' cyclic AMP stimulation of thyroid glucose oxidation and phospholipid synthesis.


Complexes of chicken heart lactic dehydrogenase with coenzymes and substrates.


Chemodectoma of lung.

Chemodectoma of lung. The histological and histochemical features of a primary chemodectoma of lung are described and compared with previously reported pulmonary chemodectomata. The tumour is of interest because, although there is physiological evidence that chemoreceptors are present in the lung, they have not yet been located anatomically.


Optimal concentration of nicotinamide adenine diphosphate in pyruvate assays.


Ossification in the sternum as a means of assessing skeletal age. The centres of ossification in the sternum were investigated to determine whether there was any correlation between ossification in the sternum and chronological age. But this proved to be of no value in assessing skeletal age, although it may be of use in assessing skeletal maturity for pathological or forensic purposes.


Histopathology of fatal adenovirus infection of the respiratory tract in young children. Type 7 adenoviruses were isolated from lung tissue obtained at the necropsies on five children aged 8 to 15 months. The clinical and radiological findings were those of bronchiolitis and pneumonia. Four children had an extensive necrotizing bronchitis and bronchiolitis. The surviving bronchial epithelium, and all bronchial epithelium in the fifth case, was proliferating. There was evidence that this proliferation was a virus-induced effect and not reparative. There was virus tropism to alveolar lining cells with a pneumonia showing mononuclear cellular reaction, necrosis, and hyaline membranes. Distinctive necrotizing lesions were found in the bronchial glands of all cases. Two contrasting types of intranuclear inclusions were present in all lesions and their significance and inter-relationships are discussed. These pathological changes are specific for infections by a limited number of adenovirus serotypes.


Morphogenesis of testicular tumours.

Morphogenesis of testicular tumours. A case is described in which death resulted from multiple trophoblastic teratomatous metastases arising, apparently, from a seminoma of the left testis. This occurrence is discussed and related to similar cases recorded in the literature. It is concluded that these cases continue to provide some evidence for the seminomatous origin of teratomas.


Absorption of antibiotics during peritoneal dialysis in patients with renal failure. Cloxacillin, ampicillin, cephaloridine, kanamycin, and polymyxin B were administered singly in the dialysis fluid of patients having peritoneal dialysis for chronic renal failure. Therapeutic blood levels could be attained with all the drugs by the end of 12 hours of dialysis, using a concentration in the dialysis fluid which did not produce any local toxic symptoms. Absorption rates across the peritoneum were higher for cephaloridine, ampicillin, and cloxacillin than for kanamycin and polymyxin B. The serum half-lives after termination of dialysis were in excess of 11 hours, except that for cloxacillin which had a half life of about 2.5 hours.


A study in vitro of the sensitivity to antibiotics of Bacteroides fragilis. During a two-year period of observation Bacteroides species were isolated from specimens of pus and vaginal swabs from 115 patients in this hospital. Thirty-five representative strains proved on examination to be Bacteroides fragilis. Minimal inhibitory and minimal bactericidal concentrations of six antibiotics for these strains were determined. All strains were resistant to streptomycin, neomycin, and polymyxin, slightly sensitive to penicillin and ampicillin, and fully sensitive to tetracycline, chloramphenicol, erythromycin, and lincomycin. The minimum bactericidal concentrations of chloramphenicol, erythromycin, and lincomycin were two to four times the minimal inhibitory concentrations. Tetracycline failed to exert any consistent bactericidal effect.The treatment of patients with infections caused by B. fragilis is discussed in the light of the findings in vitro.


Cytochrome oxidase activity in the gastric mucosa of patients with iron deficiency. Cytochrome oxidase activity was assessed histochemically in gastric biopsies taken from 22 iron-deficient patients. Activity was strong only in parietal cells, and the total amount of activity appeared to be proportional to the number of parietal cells present in the sections. No evidence was found of diminished activity within parietal cells.


Ovarian phospholipid composition and incorporation of [1-14C] acetate into the phospholipid fatty acids of ovaries from non-pregnant and pregnant rabbits.


Mechanism of resistance to enteroviruses of some primate cells in tissue culture. Two cell lines, M10-45-2 and L-41, were studied, each of which possessed specific resistance either to poliovirus or to coxsackievirus. Infection of M10-45-2 cells with poliovirus ribonucleic acid (RNA) and L-41 cells with infectious coxsackievirus RNA was accompanied by production of complete viruses in each of the resistant cell lines. During incubation of the cells with the virus to which they were resistant, the amount of infectious virus did not decrease. Treatment with glycine-HCl buffer solution (pH 2.5) of resistant M10-45-2 cells after incubation with poliovirus at 0 C did not result in recovery of infectious virus, although such release did take place after treatment of sensitive M10 cells. Infection of resistant cells with virus containing poliovirus RNA and coxsackievirus proteins resulted in production of poliovirus in M10-45-2 cells but not in L-41 cells. The resistant cells are apparently unable to adsorb the virus to which they are resistant.


Destruction of L cells by Mengo virus: use of interferon to study the mechanism. Interferon, when added to L cells, inhibited the synthesis of infectious Mengo viral ribonucleic acid, hemagglutinins, and infectious virus by 85 to 95%. Serum-blocking antigens were also reduced by the action of interferon, but threefold excess amounts of these antigens accumulated in interferon-treated cultures above the amounts expected for the quantity of infectious virus that was produced in these cultures. Radioautographic analysis showed that 28 to 36% of the cells of an interferon-treated population synthesized viral ribonucleic acid and 36 to 47% produced viral antigens as determined by an immunofluorescence technique. Despite the reductions in synthesis of viral components, all cells in an interferon-treated culture underwent cytopathic effects at the same time as cells in infected cultures which had not been treated with interferon. The results are compatible with the hypothesis that the cell destruction which results from the infection of L cells with Mengo virus is due to a protein which is coded for by the virus but is not a component of the mature virion.


In vitro chick embryo cell response to strain MC29 avian leukosis virus. Strain MC29 avian leukosis (myelocytomatosis) virus induced infection, elaboration of virus, and morphological alteration in chick embryo cells in vitro. Virus liberation began within 18 hr, morphological change was detectable at about 40 hr, and the cultures could be completely altered within 80 hr after infection. Altered cells were about half the volume and grew at approximately twice the rate of uninfected elements. The output of virus estimated by electron microscopy was about 140 particles per cell per hr. Deoxyribonucleic acid remained constant, but ribonucleic acid increased in both infected and control cells in adjustment to culture environment. The rates of uptake and incorporation of (3)H-uridine and the incorporation of (3)H-thymidine increased in the infected cells with onset of morphological change but were unaffected by processes of infection and virus elaboration per se. Incorporation of a (14)C-amino acid mixture was slightly greater in the infected than in control cells. The speed of continuity of infection and massive morphological alteration constitute a unique response to avian tumor viruses, and the system gives promise of singular value for detailed studies of the processes of infection and morphological change.


Herpes simplex virus-host cell relationships in organized cultures of mammalian nerve tissues. Studies on the replication of herpes simplex virus in organized cultures of rat central nervous system (CNS) and peripheral nervous system (PNS) tissue demonstrated synthesis of intra- and extracellular virus, as determined by plaque assay on HEp-2 cells. Newly synthesized intracellular virus appeared 12 to 14 hr after inoculation of CNS, followed 10 hr later by the appearance of extracellular virus. In PNS cultures, where higher inputs of virus were introduced, intracellular virus appeared 6 to 8 hr after inoculation, followed by extracellular virus 12 hr later. Polykaryocyte formation was observed in CNS and PNS tissue involving neuroglial, meningeal, or Schwann cells. Neuron somas did not participate in polykaryocyte formation, but they underwent progressive morphological changes starting with increased cytoplasmic granularity followed by nucleolar distortions and disintegration, margination of nuclear chromatin, and the appearance of intranuclear inclusions. Finally, all recognizable cellular detail was lost. Immune serum globulin failed to inhibit both the progressive nature of the cytopathic effect and the synthesis of intracellular virus. These findings are discussed in relation to other in vitro systems, as well as to disease processes in man and animals.


Effects of ultraviolet irradiation on the transforming and plaque-forming capacities of simian adenovirus SA7.


Enhancing effect of diethylaminoethyl-dextran on the focus-forming titer of a murine sarcoma virus (Harvey strain).


Growth inhibition of Newcastle disease virus upon superinfection of poliovirus in the presence of guanidine.


Role of cyclic AMP in hormone actions.

Role of cyclic AMP in hormone actions.


Abnormalities of vitamin B12 and folic acid metabolism--their influence on the nervous system.


Effects of hypophysectomy of ACTH on the changes induced by hexadimethrine bromide.


Follow-up study of 100 cases of pseudotumor cerebri.


Studies on vascular permeability in peripheral nerves. 3. Permeability changes of vasa nervorum and exudation of serum albumin in INH-induced neuropathy of the rat.


Histochemical and electron microscopic observations on "onion bulb" formations in a case of hypertrophic neuritis of 25 years duration with onset in childhood.


Development of strains of Cryptococcus neoformans resistant to nystatin, amphotericin B, trichomycin and polymyxin B.


Attempts to map the poliovirus genome by analysis of selected recombinants.


The effect of mutagens on the marker of poliovirus.


Histopathology of sarcomas induced in hamsters by clones of in-vitro SV40-transformed homologous heart cells. Attempts to correlate morphologic features and degree ofoncogenic potential.


Growth stimulation of polyoma virus treated hamster cells in soft agar by conditioned medium.


Cytotoxic, hemolytic and phospholipase contaminants of commerical neuraminidases.


Comparison of the adrenocorticotrophic activities of two synthetic polypeptides: synacthen and DW 75. The adrenocorticotrophic activities of two synthetic polypeptides, Synacthen (Ciba) and DW 75 (Sandoz), were compared in two groups of patients having a variety of arthritic disorders. Compared with Synacthen, injected either intramuscularly or intravenously, intravenous DW 75 was found to have a longer duration of adrenocorticotrophic activity (measured by plasma 11-hydroxycorticosteroid levels) in both groups of patients, while intramuscularly administered DW 75 did not appear to be absorbed well.


Evaluation of physical maturity at adolescence.


A study of the mechanism of polymyxin M inactivation.


Latent virus infections in primate tissues with special reference to simian viruses.


Transplacentar induction of neurogenic malignomas by 1,2-diethyl-hydrazine, azo-, and azoxy-ethane in rats.


Responses of oculomotor nucleus and marginal gyrus in sleep.


Carcinogenicity of tetraethyl lead.

Carcinogenicity of tetraethyl lead.


Immunofluorescent localization of beta-corticotropin in the rat pituitary.


In vitro comparative evaluation of metronidazole and other anti-amoebic drugs against Entamoeba histolytica.


Anti-variola and anti-vaccinia principles from seeds of Banakadali (Ensete superbum, Cheesm, Musaceae).


Transformation of mammalian cells by animal viruses.


Recurrent inhibition in the giant-fibre system of the crayfish and its effect on the excitability of the escape response.


Gas gangrene abscess of the brain.

Gas gangrene abscess of the brain.


Influence of vasopressin and of a crude CRF preparation on pituitary ACTH-release in posterior-lobectomized rats.


The chromophore and partial structure of viomycin.


Pulmonary stenosis in patients with the Turner phenotype in the male.


Effect of an exercise program on the perennially asthmatic child.


Kinetic studies on the cyclic phosphodiesterase with 3' nucleotidase activity from Proteus mirabilis.


Structural requirements for the action of steroids as inhibitors of electron transfer.


Stimulation of glucose utilization and inhibition of lipolysis by polyene antibiotics in isolated adipose cells.


Rhein: an inhibitor of mitochondrial oxidations.


Spectrophotometric analysis of rat liver lactate dehydrogenase-coenzyme and coenzyme analog complexes.


FDPases of the castor bean endosperm and leaf: properties and partial purification.


Synthesis of indoleacetic acid from tryptophan via indolepyruvic acid in cell-free extracts of pea seedlings.


Inactivation of yeast alcohol dehydrogenase by N-alkylmaleimides.


Studies on dextran and dextran derivatives. XIV. Metal binding properties of succinyl- and phthalyl-dextrans.


Evaluation of N1-(omega-bromoacetamidoalkyl)nicotinamides as inhibitors of dehydrogenases.


Arenal cells in tissue culture. I. Assay of steroid products; steroidogenic responses to peptide hormones.


Glycoprotein biosynthesis: studies on the receptor specificity of the polypeptidyl: N-acetylgalactosaminyl transferase from bovine submaxillary glands.


Purification and some properties of yeast citrate synthase.


Mononeuritis multiplex in lepromatous leprosy.


Thyrotropin hormone deficiency with a peripheral neuropathy.


A denaturation map of polyoma virus DNA.


Motor innervation of the coronary arteries of the cat. 1. The effect on coronary vascular resistance of selective stimulation of the Adelta, B and sC fibre groups of the post-ganglionic cardiac sympathetic nerves was studied. The main left coronary artery was perfused at constant flow. The oxygen saturation of coronary sinus blood was measured continuously.2. Stimulation of the peripheral ends of the cut Adelta afferent fibres, normally excited by myocardial ischaemia, had no effect on coronary vascular resistance; these fibres do not evoke an axon reflex in the heart.3. Stimulation of the preganglionic B fibres that run without synapse through the stellate ganglion also had no measurable effect on coronary resistance.4. Stimulation of the post-ganglionic sC fibres of the cardiac sympathetic nerves caused coronary vasodilatation which occurred earlier than, and was initially independent of the decrease in coronary sinus oxygen saturation.5. The injection of noradrenaline into the perfusion system had the same effect as stimulation of the sC fibres. In the K(+)-arrested heart, both noradrenaline and stimulation of the post-ganglionic nerves elicited coronary vasodilatation without changing the oxygen saturation of coronary sinus blood.6. The intracoronary injection of acetylcholine caused coronary vasodilatation followed by an increase of coronary sinus oxygen saturation.7. Vagal stimulation caused brady cardia and a fall in coronary resistance.8. Propranolol blocked coronary vasodilatation elicited by sympathetic stimulation or noradrenaline without affecting the vasodilatation due to myocardial ischaemia or acetylcholine. Atropine blocked coronary vasodilatation evoked by acetylcholine without affecting that due to ischaemia or noradrenaline. Therefore smooth muscle of the coronary arteries has at least three different receptor sites from which vasodilatation can be elicited.9. Hypertensin caused coronary vasoconstriction.10. The presence of sympathetic cholinergic vasodilator fibres innervating the coronary arteries could not be demonstrated.


Histopathology of an enzootic diarrheal disease in cattle.


Spin-label studies of the excitable membranes of nerve and muscle.


Conformation studies with polypeptides by rotatory dispersion and thin-film dialysis.


"Big insulin": a new component of plasma insulin detected by immunoassay.


Enzymatic joining of polynucleotides, V. A DNA-adenylate intermediate in the polynucleotide-joining reaction.


Mitochondrial origin of membrane-associated heterogeneous RNA in HeLa cells.


Incorporation of oligodeoxynucleotides into DNA.


Inducers of interferon and host resistance, V. In vitro studies.


Rescue of infectious SV40 after fusion between dfferent SV 40-transformed cells.


The role of Factor B in the energy transfer reactions of oxidative phosphorylation.


The in vivo life span of normal and preneoplastic mouse mammary glands: a serial transplantation study.


Polypeptide cleavages in the formation of poliovirus proteins.


Treatment of intracranial gliomas and metastatic carcinomas by local application of cytostatic agents.


[Diagnosis of adenovirus infection by use of fluorescent antibody technique].


Epidemiology of adenovirus-associated virus infection in a nursery population.


Enteroviruses within an estuarine environment.


The use of rivanol treatment for removal from sera of nonspecific inhibitors of enterovirus and reovirus hemagglutination.


The carrier state in pertussis.

The carrier state in pertussis.


Oral activity cycles in mild chronic schizophrenia.


Foot-and-mouth disease.

Foot-and-mouth disease.


Metabolism of animal cells infected with nuclear DNA viruses.


Immunofluorescent study of the inhibitory effect of N1-N1-anhydro-bis (beta-hydroxyethyl) biguanide (ABOB) on the multiplication of Sendai virus in tissue cultures.


Non-chromaffin paraganglioma of the kidney with distant metastases.


Histochemical demonstration of certain phosphomonoesterases in the Vater-Pacini receptors in cats.


Formate dehydrogenase from the pea seedling.


Cu2+ in non axial-field: a model for Cu2+ in copper enzymes.


TSH-like actions of dibutyryl-cAMP on isolated bovine thyroid cells.


Primary periodic paralysis associated with hyperkalemic and hypokalemic attacks and with hemolytic bilirubinemia.


Corticotrophin secretion capacity in multiple sclerosis.


Effect of hydrocortisone and ACTH upon the bioelectric activity of spinal cord.


A social and clinical study of geriatric admissions to a psychiatric hospital.


The application of Triton X 100 colloid scintillation counting in biochemistry.


Protection by anoxia of the scrapie agent and some DNA and RNA viruses irradiated as dry preparations.


Temperature-sensitive events in the growth of variola virus in HeLa cells.


The effect of 5-bromodoxyuridine and actinomycin D on the multiplication of transmissible gastroenteritis virus.


Morphological transformation of human embryonic liver cells by simian virus 40.


Observations on the fine structure and replication of varicella virus in cultivated human amnion cells.


The inhibition of decubitus ulceration with ACTH.


Cystosarcoma phyllodes: a unique case with multiple unilateral lesions and ipsilateral axillary metastasis.


Electron spin resonance linewidths in met myoglobin.


Relation between blood groups and resistance to infection with influenza and spome picornaviruses.


Demonstration of a pseudo-cowpox virus in New Zealand.


Brush border particulates of renal tissue. Particulates containing a large part of the alkaline phosphatase activity of renal tissue were separated from homogenates and from ribosomal preparations by zonal centrifugation. The particles had a high content of phospholipid and cholesterol that was not removed by treatment with I percent deoxycholate. Enzymatic activities concentrated with the particles were the alkaline phosphatase, a peptidase resistant to proteolysis, glucose-6-phosphatase, inorganic pyrophos-phatase, and adenosine triphosphatase. The particles accumulated leucine with no stimulation from soluble factors and with inhibition by other amino acids; the accumulation was stimulated by adenosine triphosphate and was not inhibited by puromycin. The particles appear to be derived from the membranes of the brush borders of tubular cells.


Basis for the acquisition of malignant potential by mouse cells cultivated in vitro. Balb/c mouse embryo lines maintained in culture for over 200 generations under conditions that minimize cell-cell contact do not become tumorigenic. Lines cultivated under conditions where there is extensive cell contact become tumor-producing within 30 generations. The tissue-culture property that correlates best with tumorigenicity is the loss of contact inhibition of cell division.


Visual cortex neurons: response to stimuli during rapid eye movements. While awake, unanesthetized monkeys held their eyes stationary, a motionless or slowly moving stimulus falling on the receptive field of striate cortex neurons produced an excitatory response. When a rapid eye movement was made across the same stimulus, many of these neurons continued to give an excitatory response. But the discharge of other neurons was unchanged or was suppressed during the eye movement.


Radiobiological damage: a new class identified in barley seeds stored after irradiation. A new class of radiobiological damage has been identified in irradiated barley seeds. Consisting of both physiological and genetic damage, it appears to be independent of oxygen and long-lived free radicals, and develops very slowly during storage after irradiation. Increasing the storage temperature accelerates its development.


Cellular aspects of the control of color changes. AAAS Symposium, 29 December 1968 Dallas, Texas.


Zoonoses in Britain: some present and potential hazards to man. Skin diseases.


A short communication on another simplified micromethod for performing chromosome preparations from peripheral blood.


Granular cell tumors (myoblastomas) involving the bronchus and skin. Report of a case.


Studies on the pathogenesis of parainfluenza type 3 virus infection in hamsters.


Immunological relationship between herpes simplex virus and B virus.


Decreased hepatic microsomal drug metabolism after the injection of a mixture containing somatotropin, corticotropin and prolactin.


Energy-linked reduction of nicotinamide adenine dinucleotides in cells of Rhodospirillum rubrum.


Reconstitution of succinic-coenzyme Q reductase and succinic oxidase activities by a highly purified, reactivated succinic dehydrogenase.


Intermittent and pulsating exophthalmos.


Changes in DNA and RNA in the rat adenohypophysis following bilateral adrenalectomy, sham adrenalectomy, and the chronic injection of cortisol.


Restoration of adrenal-regeneration hypertension in corticosterone-blocked rats by ACTH.


Chemotherapy in dental practice. Polypeptide antibiotics. 3. Polymyxin.


The formation and reactions of hydrogen atoms in gamma-irradiated deoxyribonucleic acid gels.


Coenzyme A-linked aldehyde dehydrogenase from Escherichia coli. I. Partial purification, properties, and kinetic studies of the enzyme.


Identification and analysis of multiple glycine transport systems in isolated mammalian renal tubules.


Oligonucleotides produced by digestion of KB cell ribosomal 5 S ribonucleic acid with specific nucleases.


The divergence in reactivity of aminoacyl transfer ribonucleic acid synthetases of Escherichia coli with hydroxylamine.


A study of the threonyl adenylate complex with threonyl transfer ribonucleic acid synthetase and its reaction with hydroxylamine.


The role of lysyl residues in the structure and reactivity of cytochrome B5 reductase.


Contiguous but dissimilar epithelial neoplasms of the urinary bladder. An unusual tumour of the urinary bladder is described. It consists of two distinct nodules each with a quite different histological appearance, one being mucus-secreting adenocarcinoma, the other transitional cell carcinoma. Where the nodules are in contact there is little intermingling of the two histological patterns and it is suggested that the findings are probably due to the collision of two separate neoplasms. Metaplastic changes are present in the bladder mucosa adjacent to the tumour. The significance of these changes in the pathogenesis of adanocarcinoma is discussed.


Cytomegalic inclusion disease in the adult. The distribution of infected cells in 13 necropsies on adults with cytomegalovirus disease is reported and discussed in relation to the local damage caused by the infection and predisposing factors. The distribution, with lung as the most common site, but other organs such as thyroid, liver, and colon not infrequently involved, agrees fairly well with other series. No heavy infection confined to one organ was found, and it is concluded that a truly localized infection in the adult probably does not occur. Evidence of destructive inflammatory changes attributable to the virus was scanty; a lymphocyte and plasma cell infiltrate was sometimes found, and microglial nodules were found in a case with inclusions in the brain. In one case heavy cytomegalovirus infection in the colon was associated with, and probably caused, marked colonic ulceration which led to the death of the patient. THE MAJOR PREDISPOSING FACTOR TO INFECTION IN THIS SERIES WAS CORTICOSTEROID THERAPY: eight of the 13 patients had been treated with steroids and one had Cushing's syndrome associated with a bronchial carcinoma. Three of the other four patients had a very few inclusions only. Adrenal involvement was not found in any of the steroid-treated patients but was present in two of the four patients not treated with steroids where adrenal sections were available. It is concluded that most adult patients with cytomegalovirus infection have impaired immune mechanisms, and that heavy infection may rarely lead to a clinically serious disease.


Glucagon test for insulinoma: a chemical study in 25 cases. The capillary blood glucose response to lmg of intramuscular glucagon was determined in 13 patients with insulinoma and in 33 normal controls; the insulinoma patients showed a normal initial rise, but this was followed by an abnormally large fall, reaching hypoglycaemic levels between 90 and 180 minutes in every case. In 14 insulinoma patients the response of venous blood glucose and also plasma insulin to lmg of intravenous glucagon was compared with 10 normal controls; there was an abnormally large rise of plasma insulin in 10 of the 14 patients, and in the majority the venous blood glucose was below normal throughout the test. In these 14 patients the plasma insulin response was also determined after oral and intravenous glucose, after oral leucine, and after intravenous tolbutamide, and the value of these tests in the recognition and differential diagnosis of insulinoma was compared with that of the intravenous glucagon test.


Routine multipurpose gas chromatographic assay for urinary corticosteroids. THE USE OF A ROUTINE ASSAY FOR URINARY STEROIDS IS DESCRIBED THAT IS THOUGHT TO SATISFY THE FOLLOWING NEEDS: the measurement of low levels of 17-hydroxycorticosteroid excretion; the assessment of adrenocortical activity during prednisolone therapy; the diagnosis of the adrenogenital syndrome and the monitoring of its treatment using any available urine specimen; the very early diagnosis of pregnancy using any available urine specimen. It may also prove of value in the assessment of progesterone secretion and metabolism during pregnancy.


Studies on the cytotoxic action of silica dusts on macrophages in vitro.


Polyvalent parainfluenza CF antigen for serodiagnosis.


Evidence of an infective component sensitive to ribonuclease in the brain homogenate fo mice inoculated with MM virus.