Clearing-factor lipase in adipose tissue. Factors influencing the increase in enzyme activity produced on incubation of tissue from starved rats in vitro. 1. Evidence is presented that the increase in clearing-factor lipase activity that occurs when adipose tissue from starved rats is incubated in a defined medium in vitro is due to an increase in the total enzyme content of the system. It is shown that the clearing-factor lipase activity rises to reach a plateau level where, it is suggested, rates of enzyme synthesis and of enzyme destruction become balanced. 2. The presence of heparin in the incubation medium results in the extraction of part of the clearing-factor lipase originally present in the adipose tissue and this could provide the stimulus for the increase in total enzyme content. 3. Glucose is required in the incubation medium at a very low concentration. It can be replaced by fructose, but not by pyruvic acid, lactic acid, glyceric acid or dihydroxyacetone. 4. Adrenaline and corticotrophin inhibit the increase in enzyme activity when they are present in the incubation medium. 5. The high clearing-factor lipase activity associated with adipose tissue of fed rats is decreased by 50% within 3hr. of the injection of puromycin.


Studies in rapidly labelled ribonucleic acid in cell fractions and in tumour tissues. 1. Twenty minutes after injection of [(3)H]orotic acid into rats the rapidly labelled RNA from the liver is mainly associated with the nuclear fraction and little with the ribosomal cytoplasmic fraction. 2. The thermal denaturation of RNA from the fractions was not as reversible as that of the RNA extracted from whole liver. 3. Rapidly labelled RNA is synthesized by cells from a transplantable hepatoma when incubated in the presence of [(3)H]uridine and, after extraction and centrifugation, the label is present in three main fractions: one which sediments to the bottom of a gradient and is associated with DNA, a second which sediments to the heavy side of the 28s RNA, and a third which has a peak of activity between 28s RNA and 18s RNA and is associated with DNA. 4. After labelling and extraction of the RNA from Ehrlich ascites cells the distribution of radioactive components is similar to that of the material from the hepatoma cells. 5. The difference between the tumour cells and liver is due to some extent to the method of homogenizing the tissues and the nature of the components is discussed.


Rapidly labelled ribonucleic acid from rat liver. Studies in the attachment to ribosomes and stimulation of the incorporation of amino acids into polypeptides in cell-free systems. 1. Rapidly labelled RNA from rat liver, either as a complex with DNA (m-RNA-DNA) or with ribosomal RNA (m-RNA-RNA) binds to ribosomes in the polysome region. No binding could be demonstrated with ribosomal RNA or native DNA from Bacillus subtilis. 2. With ribosomes from rat liver, Escherichia coli or hepatoma the m-RNA-DNA stimulated incorporation of amino acids with rat-liver ribosomes only, whereas the m-RNA-RNA complex was effective with ribosomes from E. coli or the hepatoma. 3. Polyuridylic acid was effective as messenger RNA with all three ribosomes but much greater stimulation was obtained with ribosomes from E. coli and the hepatoma. 4. The degree of incorporation of phenylalanine with polyuridylic acid and ribosomes from a hepatoma was decreased by about 50% when ribosomal RNA was present.


Unusual group of tumours among schoolgirls.


The isolation of nuclei from rat liver during aminoazo dye carcinogenesis.


Clostridial sepsis.

Clostridial sepsis.


Treatment of choriocarcinoma.

Treatment of choriocarcinoma.


Chromosome studies of human brain tumors.


Abrupt withdrawal of adrenal corticosteroids in patients with rheumatoid arthritis: the clinical observations of twelve cases.


Diurnal variation in adrenal cortical function.


Insecticidal compounds. Effects on replication of vaccinia and polio viruses in human chang-strain liver cells.


Etiology of the acute infectious respiratory diseases. With specific reference to the rhinoviruses.


Control of acute viral respiratory diseases. Effects of infection, vaccines, and chemotherapy.


Interferon synthesis in x-irradiated animals. I. Depression of circulating interferon in C3H mice after whole-body irradiation.


Histological changes in interrenal tissue of the goldfish, Carassius auratus, following x-irradiation.


Histochemical demonstration of cytochrome oxidase activity in the lens.


Peripheral neuropathy of sensorimotor type associated with malignant disease.


Mumps and enteroviral meningitis in Toronto, 1966. Of 52 children admitted to hospital for apparently typical mumps meningitis in 1966, 50 had their cerebrospinal fluid (CSF) examined. In only 17 was the mumps virus isolated from the CSF. Mumps antihemagglutinin conversions or increments were detected in 32 subjects including 10 whose CSF yielded virus. Antibody conversions were found in 16 patients and fourfold increments in another nine whose serum pairs were collected only one to four days apart. Initial sera from 20 patients were obtained three days or less after the onset of meningitis. Antibody increments were frequently noted about one day after defervescence and clinical improvement. Interferon was detected in CSF from two of eight patients, both of whom yielded virus. Enteroviruses were isolated from CSF and/or feces in seven of 15 cases of aseptic meningitis which occurred between July and October. Six patients including three virus excretors showed enteroviral neutralizing antibody increments during convalescence. The dominant enteroviral serotype was coxsackievirus A9.


Reconstitution of cytochrome C oxidase from a copper-depleted enzyme and Cu.


Vitamin E deficiency in the rat. 3. The relationship of nicotinamide adenine dinucleotide to respiratory decline.


Mucopolysaccharide sulfation in chick embryo cartilage. I. Properties of the sulfation system.


Purification and characterization of poliovirus-induced infectious double-stranded ribonucleic acid.


Enzymes of plasma membranes of liver.

Enzymes of plasma membranes of liver.


Acyl carrier protein. IX. Acyl carrier protein hydrolase.


Tryptophan as intermediate in dehydrogenase action. 3. Evidence for complete cycle of hydrogen transfer between substrate and tryptophanyl residues in rabbit muscle lactate dehydrogenase.


The isolation and metabolism of brown fat cells.


A long chain terpenyl pyrophosphate synthetase from Micrococcus lysodeikticus.


Studies on the renal glomerular basement membrane. Nature of the carbohydrate units and their attachment to the peptide portion.


The inhibition by phlorizin of kidney microsomal inorganic pyrophosphate-glucose phosphotransferase and glucose 6-phosphatase.


Studies on cytochrome c peroxidase. IX. The reaction of ferrimyoglobin with hydroperoxides and a comparison of peroxide-induced compounds of ferrimyoglobin and cytochrome c peroxidase.


Antiviral substances in plants of the mint family (labiatae). II. Nontannin polyphenol of Melissa officinalis.


Polyoma virus-induced "complement-fixing antigen" in tumors and infected cells and detected by immunofluorescence.


Infrequent C-F antibody response in mice with adenovirus type 12 tumors.


Effect of pituitary and gonadal hormones on Friend Virus Disease in mice.


SV-40 virus growth and cytopathogenicity in a serial rabbit kidney cell line.


Inapparent herpes simplex virus infection in inoculated rabbits.


Vitamin D deficiency in adult quail and chickens and effects of estrogen and testosterone treatments.


Recovery of an adenovirus from a feral rodent Peromyscus maniculatus.


Effect of thyroxine administration on enzymes associated with glucose metabolism in the liver.


Mitigated teratogenicity of thio-TEPA in goldthioglucose obese mice.


Transformation of bovine cells in vitro by polyoma virus, and the properties of the transformed cells.


Effect of a low sodium diet on aldosterone-stimulating activity of angiotensin II in dogs.


Increased susceptibility of Down's syndrome fibroblasts to transformation by SV40.


Studies on the inability of rhinovirus to survive and replicate in the intestinal tract of volunteers.


Yellow bone marrow as adipose tissue.

Yellow bone marrow as adipose tissue.


Immunologic response in hamsters to homologous tumor antigens measured in vivo and in vitro.


Virological findings during the smallpox outbreak in Stockholm in 1963.


Studies on oral enzymes. IV. Fractionation and characterization of various hydrolytic enzymes in human saliva.


Effect of angiotensin on renal function in the rat.


C-glycosylflavonoids. The chemistry of aspalathin. 1. The isolation of aspalathin, the principal phenolic constituent in the leaves of Aspalathus linearis, is described and its properties are discussed. 2. The compound has been identified as 3'-C-beta-d-glucopyranosyl-2',3,4,4',6'-pentahydroxydihydrochalcone by the preparation and analysis of various derivatives, by photochemical oxidation to 2,3-dihydroiso-orientin and by nuclear-magnetic-resonance studies.


Carcinoid-islet cell tumors of the duodenum.


Acute conjunctivitis among Cairo children. Decrease in K.W. and gonococcal infections and increase in bacteriologically negative cases.


Epithelial cytoplasmic pseudo inclusions in conjunctivitis.


Phosphoserine phosphatase of vertebrates.


Ethanol-induced alterations of norepinephrine metabolism in man.


Liver parenchymal cell injury. V. Relationships between patterns of chloromethane-C 14 incorporation into constituents of liver in vivo and cellular injury.


Bronchial asthma in infants and children due to food and inhalant allergies.


Bicarbonate and the binding of iron to transferrin.


Antibody responses of rhesus (Macaca mulatta) monkeys experimentally infected with coxsackieviruses of group B and group A, type 9. II. Heterotypic antibody responses to echoviruses, polioviruses and reovirus type 1.


Antibody and gland studies in cortisone and ACTH-injected birds.


A serologic study of herpesvirus hominis strains by microneutralization tests.


Granular cell myoblastoma of the larynx.


Laryngeal haematomas due to coagulopathies.


Transplantability of primary tumors of RPL12 virus-induced lymphoid leukosis.


Induction of tumors in adult hamsters with simian virus 40.


Diphosphopyridine nucleotide in the treatment of schizophrenia.


Surveillance of respiratory virus infections among Alaskan Eskimo children.


A cutaneous clue to mammary carcinoma. Paget's disease of the nipple.


Gas gangrene following an adrenaline-in-oil injection into the left thigh with survival.


Vulvar fibroadenoma from aberrant breast tissue. Report of 2 cases.


Effects of nonsuppressible insulin-like activity and insulin on glucose oxidation and lipolysis in the isolated adipose cell.


Hemifacial spasm.

Hemifacial spasm.


The chemistry of pseudouridine.

The chemistry of pseudouridine.


Calcifying mucinous adenocarcinoma of the stomach.


Studies on the relationship between adeno-associated virus type I (AAV-1) and adenoviruses. I. Replication of AAV-1 in certain cell cultures and its effect on helper adenovirus.


Studies of three variants of Mengo encephalomyelitis virus. IV. Affinities for mouse tissues in vitro and in vivo.


Fragmentation of the nucleus in Rous sarcoma virus-infected chick embryo cells.


Enzymologic approach to the diagnosis of death by drowning. Preliminary report.


The bacteria Mima-herellea. Isolation and clinical significance in a general hospital.


Isolation of cytomegalovirus from tissues of condyloma acuminatum.


Cytomegalic inclusion disease. The fetal disorder.


Congenital spongioblastoma of the pons.

Congenital spongioblastoma of the pons.


Concentration of viruses on aluminum and calcium salts.


Studies of infantile diarrhea in Karachi, Pakistan. II. Multiple virus isolations from rectal swabs.


The uptake of enteroviruses by the New Zealand marine blue mussel Mytilus edulis aoteanus.


Histological studies of the monkey neurovirulence of group B arboviruses. 3. Relative virulence of selected viruses.


Effect of ischemia on conduction of nerve fibers of varying diameters.


Effect of interferon on the production and action of viral RNA polymerase.


Piericidin A: a new inhibitor of mitochondrial electron transport.


Evidence for uracil nucleotide-rich segments in the RNA molecule of the virus BAI strain A (avian myeloblastosis).


Observations on the actin content of the rabbit myofibril. 1. On extraction of whole muscle by the procedure of Hasselbach & Schneider (1951), the amount of actin that passes into solution seems to account for little more than 10% of the protein content of the myofibrils. 2. Extraction of isolated myofibrils with suitable media that allow identification and estimation of dissolved proteins seems to give about the same yield of actin (10-13% of the total). 3. A comparatively large residue of myofibrillar components remains after extraction. The amount of actin present in the residue can be only hypothetical.


The polyphosphoinositides and other lipids of peripheral nerves. 1. A detailed lipid analysis of the peripheral nerves of the crab (claw, leg), lobster (claw, leg), cow (splenic), hen, rabbit, sheep and monkey (sciatic) is presented. 2. The so-called ;myelinic lipids', cholesterol, sphingomyelin, ethanolamine plasmalogen and phosphatidylserine, occurred in the highest proportion in the lipids of vertebrate myelinated nerves, whereas the percentage of lecithin was greatest in the lipids of non-myelinated nerve fibres of both vertebrates and invertebrates. 3. Triphosphoinositide was found in all nerves examined and its concentration in the extracted lipids supports the concept that it is predominantly localized in the myelin sheath. 4. In crustacean nerve 12-14% of the phospholipids was in the form of alkyl ether phospholipids, which in the lobster were approximately half choline-containing and half ethanolamine-containing.


The kinetics and mechanism of liver alcohol dehydrogenase with primary and secondary alcohols as substrates. 1. The activity of liver alcohol dehydrogenase with propan-2-ol and butan-2-ol has been confirmed. The activity with the corresponding ketones is small. Initial-rate parameters are reported for the oxidation of these secondary alcohols, and of propan-1-ol and 2-methylpropan-1-ol, and for the reduction of propionaldehyde and 2-methylpropionaldehyde. Substrate inhibition with primary alcohols is also described. 2. The requirements of the Theorell-Chance mechanism are satisfied by the data for all the primary alcohols and aldehydes, but not by the data for the secondary alcohols. A mechanism that provides for dissociation of either coenzyme or substrate from the reactive ternary complex is described, and shown to account for the initial-rate data for both primary and secondary alcohols, and for isotope-exchange results for the former. With primary alcohols, the rapid rate of reaction of the ternary complex, and its small steady-state concentration, result in conformity of initial-rate data to the requirements of the Theorell-Chance mechanisms. With secondary alcohols, the ternary complex reacts more slowly, its steady-state concentration is greater, and therefore dissociation of coenzyme from it is rate-limiting with non-saturating coenzyme concentrations. 3. Substrate inhibition with large concentrations of primary alcohols is attributed to the formation of an abortive complex of enzyme, NADH and alcohol from which NADH dissociates more slowly than from the enzyme-NADH complex. The initial-rate equation is derived for the complete mechanism, which includes a binary enzyme-alcohol complex and alternative pathways for formation of the reactive ternary complex. This mechanism would also provide, under suitable conditions, for substrate activation or substrate inhibition in a two-substrate reaction, according to the relative rates of reaction through the two pathways.


Abolition by actinomycin D pretreatment in vivo of the stimulating effect of serum proteins on the protein synthesis of Yoshida ascites hepatoma in vitro.


The inhibition of ribonucleic acid polymerase by acridines. 1. The aminoacridines, proflavine (3,6-diaminoacridine) and 9-aminoacridine, and a hydrogenated derivative, 9-amino-1,2,3,4-tetrahydroacridine, were shown to inhibit in vitro the DNA-primed RNA polymerase of Escherichia coli. The inhibition is strong with both proflavine and 9-aminoacridine, but weak with 9-amino-1,2,3,4-tetrahydroacridine. 2. The extent to which the three acridines bind to calf-thymus DNA in the enzyme medium was studied spectrophotometrically. The extent of binding decreases in the order: proflavine, 9-aminoacridine, 9-amino-1,2,3,4-tetrahydroacridine. Some evidence was also obtained for interaction between the nucleoside triphosphate substrates and proflavine or 9-aminoacridine; no such interaction was detectable with 9-amino-1,2,3,4-tetrahydroacridine. 3. Although the amount of acridine bound to DNA increases with increasing inhibition, a stage is reached where an increase in acridine concentration still causes an increase in inhibition, with practically no increase in the amount bound to DNA. 4. Plots of reciprocal rates against the reciprocal of DNA concentration were linear and had a common intercept when proflavine or 9-aminoacridine was present. Similar relations were obtained when the reciprocal concentration of nucleoside triphosphates was plotted. The observations are interpreted kinetically in terms of a competitive inhibition of the enzyme by proflavine or 9-aminoacridine and of a kinetic role for the DNA analogous to ;activation'. 5. This suggests that inhibitory acridine molecules can occupy the sites on the RNA polymerase that are specific for binding the nucleoside triphosphate substrate or the bases of the DNA, when these become accessible during the copying process.


Gradient centrifugation in colloidal silica-polysaccharide media.


Preparation and properties of a cell-free system from rat skin that catalyzes sterol biosynthesis. Homogenates of epidermis from rat skin were centrifuged at 10,000 X g for 20 min. The supernatant fraction ("whole homogenate") catalyzed the demethylation of lanosterol (C(30)) to yield C(27)-sterols. The rate of reaction was measured by the rate of release of (14)CO(2) from the 4-methyl group of lanosterol. Conditions for maximal rates of demethylation were established. Addition of increasing amounts of washed microsomes to a constant amount of substrate resulted in additional release of (14)CO(2), but the release was not proportional to the amount of microsomes. Incubation with increasing amounts of microsomes treated with Triton WR-1339 yielded a proportional rate of release of (14)CO(2). The Triton-treated microsomes were frozen and stored without loss of activity. The rate of formation of (14)CO(2) was constant up to 1 hr of incubation with both Triton-treated microsomes and whole homogenate, for which the K(m) for lanosterol was 5.0 and 3.0 X 10(-5) M, respectively. Other 4-gem-dimethyl sterols were competitive inhibitors, K(i)', 2.0 and 5.5 X 10(-5) M. The enzyme system was inhibited by arsenite. 24,25-Dihydrolanosterol, 24,25-dihydrolanostenone, and squalene were demethylated by the homogenate. The whole homogenate catalyzed the incorporation of mevalonic acid, but not acetic acid, into squalene and sterols. The enzymatic properties of the sterol synthetic system from skin resemble those of similar preparations from rat liver.


Gas--liquid chromatography of silylated mixtures from lipolysis of triglycerides containing unusual fatty acyl groups. A convenient and rapid procedure involving methylation, silylation, and temperature-programmed gas-liquid chromatography (GLC) is described for analyzing unfractionated products from the hydrolysis of triglycerides with pancreatic lipase. The conditions employed for GLC were selected to provide maximum and rapid separation of silylated monoglycerides in which the acyl moieties differ in chain length or degree of substitution with oxygen-containing functional groups. Derivatives differing only in the number of double bonds present were not separated. In the GLC curves obtained, peaks representing methyl esters are generally readily distinguishable from those of other components. Therefore, the extent of lipolysis and the composition (with respect to chain length and substitution of acyl groups) of monoglycerides formed are determined simultaneously. The accuracy of the new method was demonstrated with standard mixtures and by comparison of results for several lipolysis products with data obtained by conventional procedures.


Electron microscopic study on the effect of the oxidation of ultrathin sections of Bacillus cereus and Bacillus megaterium.


Recurrent herpes in the rabbit and man.

Recurrent herpes in the rabbit and man. Herpesvirus was present in secretory glands and frequently in tears of rabbits with recurrent herpetic keratitis even in the absence of corneal lesions. In normal people, herpesvirus could be cultured from saliva and tears. Chronic virus multiplication in structures such as the lacrimal and salivary glands, rather than latency, may cause recurrent herpetic disease.


Adenovirus tumorigenesis: role of the viral genome in determining tumor morphology. Adenovirus type 12 transforms the fibroblastic BHK21 (baby hamster kidney) cell line into rounded or cuboidal cells that give rise in hamsters to undifferentiated small cell sarcomas indistinguishable from those induced in newborn hamsters by inoculation of the virus itself. In contrast. cells from this line transformed by polyoma virus retain their fibroblastic morphology and induce fibrosarcomas in hamsters. This suggests that the morphology of tumors induced by the adenovirus-transformed cells from this line may be determined by the viral genome and that such mechanism may also explain the remarkably uniform microscopic appearance which seems to characterize tumors induced in hamsters by direct inoculation of adenovirus type 12.


An antibody to oxytocin.

An antibody to oxytocin.


Mucinous adenocarcinoma of the urachus in women.


Comparative lipid composition of psittacosis and trachoma agents.


Induction of interferon by the Bour strain of trachoma in HeLa 229 cells.


Prediction of the age of maximum puberal growth in body height.


Adrenocorticotrophic hormone and erythropoietin.


The relation of cirrhosis of the liver to renal hypertension. A review of 639 autopsied cases.


The glycerol teichoic acid from the cell wall of Bacillus stearothermophilus B65. 1. A glycerol teichoic acid has been extracted from cell walls of Bacillus stearothermophilus B65 and its structure examined. 2. Trichloroacetic acid-extractable teichoic acid accounted for 68% of the total cell-wall phosphorus and residual material could be hydrolysed to a mixture of products including those characteristic of glycerol teichoic acids. 3. The extracted polymer is composed of glycerol, phosphoric acid, d-glucose and d-alanine. 4. Hydrolysis of the polymer with alkali gave glycerol, 1-O-alpha-d-glucopyranosylglycerol and its monophosphates, glycerol mono- and di-phosphate, as well as traces of a glucosyldiglycerol triphosphate and a glucosylglycerol diphosphate. 5. The teichoic acid is a polymer of 18 or 19 glycerol phosphate units having alpha-d-glucopyranosyl residues attached to position 1 of 14 or 15 of the glycerol residues. 6. The glycerol residues are joined by phosphodiester linkages involving positions 2 and 3 in each glycerol. 7. d-Alanine is in ester linkage to the hydroxyl group at position 6 of approximately half of the glucose residues. 8. One in every 13 or 12 polymer molecules bears a phosphomonoester group on position 3 of a glucose residue, the possible significance of which in linkage of the polymer to other wall constituents is discussed.


A specific sucrose phosphatase from plant tissues. 1. A phosphatase that hydrolyses sucrose phosphate (phosphorylated at the 6-position of fructose) was isolated from sugar-cane stem and carrot roots. With partially purified preparations fructose 6-phosphate, glucose 6-phosphate, fructose 1-phosphate, glucose 1-phosphate and fructose 1,6-diphosphate are hydrolysed at between 0 and 2% of the rate for sucrose phosphate. 2. The activity of the enzyme is increased fourfold by the addition of Mg(2+) ions and inhibited by EDTA, fluoride, inorganic phosphate, pyrophosphate, Ca(2+) and Mn(2+) ions. Sucrose (50mm) reduces activity by 60%. 3. The enzyme exhibits maximum activity between pH6.4 and 6.7. The Michaelis constant for sucrose phosphate is between 0.13 and 0.17mm. 4. At least some of the specific phosphatase is associated with particles having the sedimentation properties of mitochondria. 5. A similar phosphatase appears to be present in several other plant species.


The components of an alpha-glycerophosphate cycle and their relation to oxidative metabolism in the lens. 1. The concentration of ATP in a lens brei is maintained when the brei is incubated in oxygen with alpha-glycerophosphate. Lack of alpha-glycerophosphate or incubation in nitrogen causes the concentration to decrease. alpha-Glycerophosphate has some effect under anaerobic conditions but this is not sufficient to account for the maintenance in oxygen. 2. Manometric experiments show that alpha-glycerophosphate enhances the respiration of lens preparations. This respiration can be further increased by the addition of ADP and is abolished by cyanide and antimycin. The inference from these experiments is that a mitochondrial system able to oxidize alpha-glycerophosphate is present, i.e. the particulate half of the alpha-glycerophosphate cycle. 3. More than the calculated proportion of NADH is used when limiting amounts of dihydroxyacetone phosphate are added to lens tissue in spectrophotometric experiments. Dihydroxyacetone phosphate is therefore regenerated and an alpha-glycerophosphate cycle is operative. 4. A preparation of a particulate alpha-glycerophosphate dehydrogenase that takes up oxygen with methylene blue as electron acceptor is described. 5. Methods for obtaining mitochondria from lens are compared, and a useful extraction medium is defined. 6. Mitochondria with activities of the same order of magnitude as those obtained from liver, with alpha-glycerophosphate and glutamate as substrates, are prepared from epithelium detached from the capsule; some respiratory control is observed.


A study of the reaction catalysed by the liver branching enzyme. The mechanism of action of liver branching enzyme has been studied by using as substrate two polysaccharides in which the non-reducing ends had been labelled by incubation with phosphorylase and a trace amount of [(14)C]glucose 1-phosphate. After these polysaccharides had been treated with branching enzyme, their structure was analysed by periodate oxidation, by degradation with phosphorylase and amylo-(1-->6)-glucosidase and by degradation with pullulanase. All the results indicate that the branching enzyme catalyses the transfer from (1-->4)- to (1-->6)-linkage of a chain of glucose units, the minimum length of which is six glucose units. A maltodextrin containing 16 glucose units was not acted on by the branching enzyme.


Some rate constants for the phenazine methosulphate-catalysed oxidation of reduced nicotinamide-adenine dinucleotide. Kinetic measurements have been made on the phenazine methosulphate-catalysed oxidation of NADH(2) and the effects of cyanide, EDTA, Fe(2+), catalase and an unidentified mitochondrial factor on this reaction are examined. Chemical equations are formulated and values of the rate constants are determined. The data suggest the existence of an unreactive form of the reduced phenazine methosulphate; the stability and possible nature of such a compound are discussed.


Studies on nucleolar RNA fractions of the Walker and Novikoff tumors.


Some urea cycle enzymes and amino acyl-RNA synthetases of rat hepatomas.


Metabolism of continuously fed 14C-labeled N-hydroxy-N-2-fluorenylacetamide at various intervals: effect of pituitary hormones.


Antigenicity of a virus-induced murine sarcoma (Moloney).


Biological properties of a transplantable islet-cell tumor of the golden hamster. II. Insulin content of the tumor and some metabolic characteristics of the tumor-bearing animals.


Microscopic studies of the effect of epinephrine on the isolated fat cells of the rat epididymal fat pads.


Multiple harvest technic for production of parainfluenza and measles hemagglutinating antigens.


Formation and operation of the histidine-degrading pathway in Pseudomonas aeruginosa. Histidine ammonia lyase (histidase), urocanase, and the capacity to degrade formiminoglutamate, which are respectively involved in steps I, II, and IV in the catabolism of histidine, were induced during growth of Pseudomonas aeruginosa on histidine or urocanate, and were formed gratuitously in the presence of dihydro-urocanate. Urocanase-deficient bacteria formed enzymes I and IV constitutively; presumably they accumulate enough urocanate from the breakdown of endogenous histidine to induce formation of the pathway. Urocanate did not satisfy the histidine requirement of a histidine auxotroph, indicating that it probably acted as an inducer without being converted to histidine. The results imply that urocanate is the physiological inducer of the histidine-degrading enzymes in P. aeruginosa. Enzymes of the pathway were extremely sensitive to catabolite repression; enzymes I and II, but not IV, were coordinately repressed. Our results suggest a specific involvement of nitrogenous metabolites in the repression. Mutant bacteria with altered sensitivity to repression were obtained. The molecular weight of partially purified histidase was estimated at 210,000 by sucrose gradient centrifugation. Its K(m) for histidine was 2 x 10(-3)m in tris(hydroxymethyl)aminomethane chloride buffer. Sigmoid saturation curves were obtained in pyrophosphate buffer, indicating that the enzyme might have multiple binding sites for histidine. Under certain conditions, histidase appeared to be partially inactive in vivo. These findings suggest that some sort of allosteric interaction involving histidase may play a role in governing the operation of the pathway of histidine catabolism.


Mouse-protective properties of Bordetella pertussis serotypes in passive tests. In a passive protection procedure in which the ed(50) values of Bordetella pertussis antisera were determined, groups of mice were given graded intraperitoneal doses of serum, followed the next day by intracerebral challenge with 100,000 organisms. Antiserum produced with B. pertussis culture 5373, serotype 1.3, protected mice against challenge with culture 18-323, serotype 1.2.3, as effectively as did an antiserum produced with a serotype 1.2.3 culture. When two groups of mice similarly treated with pertussis immune serum were challenged with culture 353Z (serotype 1) and 18-323, respectively, much lower ed(50) values were obtained with the animals challenged with 353Z. Passive protection tests with adsorbed antiserum gave equivocal results, suggesting that some of the adsorbing antigen remained in the serum and interfered with the tests. There was no evidence that serotype is related to protection.


In vitro demonstration of humoral and cell-bound immunity against common specific transplantation atigen(s) of adenovirus 12-induced mouse and hamster tumors. A colony inhibition (CI) technique was used to demonstrate in vitro tumor-specific antigenicity common for neoplasms induced by the adenovirus type 12. Immunization of mice against syngeneic mouse adeno 12 tumor cells evoked immunity demonstrable against other adeno 12 neoplasms of mice and of hamster as well. The immunity was detected as antibody activity in the sera of specifically immunized mice and as a similar activity of the lymph node cells of these animals. The finding in transplantation experiments that infection with adenovirus types 12 and 7 induces an isograft immunity against adeno 12 tumors was confirmed by CI tests on the sera of virus-infected mice. The probable identity of the antigen detected by the CI technique and the tumor-specific transplantation antigen is discussed.


The action of lysozyme on bacterial electron transport systems.


Persistent non-cytocidal infection of BHK 21 cells by human parainfluenza type 2 virus.


Calcified soft-tissue mass in the medial aspect of the thigh.


Experimental brain tumors, with a report of those induced in dogs by Rous sarcoma virus.


Diagnosis of hypopituitarism in childhood.


Differential diagnosis of children with short stature not associated with metabolic, chromosomal, or gross nervous system defects.


Effect of treatment on the metabolism of tryptophan in childhood epilepsy.


Histochemical studies of chondrocyte function in the cartilage of the mandibular codyle of the rat.


Studies of androgens in patients with adrenocortical tumors.


Bacteriocin produced by Bordetella pertussis. Of the 24 strains of Bordetella pertussis examined, 2 produced bacteriocins that inhibited the growth of all but 2 other strains of this species. The two strains producing the bacteriocin and the two resistant strains were rough, whereas all susceptible strains were smooth. The bacteriocin was not active on the B. parapertussis or B. bronchiseptica strains tested. These bacteriocins appeared to be protein in nature, since they were heat-labile and partially inactivated by trypsin. They were antigenic but the neutralizing antibodies did not precipitate the antigens. Absorption of the antiserum with homologous cell suspensions removed the agglutinating, but not the neutralizing, antibody.


Genetic mapping of mutations affecting phosphoglucose isomerase and fructose diphosphatase in Escherichia coli. The loci on the Escherichia coli genome of mutations affecting phosphoglucose isomerase (pgi) and fructose diphosphatase (fdp) have been determined by conjugation and transduction experiments, chiefly three-factor crosses. The loci for these two constitutive enzymes of central intermediary metabolism lie in the same region of the chromosome, but they are not cotransducible. The order of some markers in this region of the chromosome is arg(ACFH) metA pgi malB uvrA fdp pyrB thr.


Pituitary-adrenocortical studies in a patient with Cushing's syndrome induced by thymoma.


Effect of angiotensin II on immunoreactive insulin.


Inhibition of renin by heparin.

Inhibition of renin by heparin.


Blood cholesterol and hydrocortisone production in man: quantitative aspects of the utilization of circulating cholesterol by the adrenals at rest and under adrenocorticotropin stimulation. A kinetic study of the conversion of blood cholesterol into hydrocortisone was carried out in two patients through prolonged infusions of cholesterol-4-(14)C. The following points appear to be established by our observations:1) The infused tracer behaved metabolically like endogenous cholesterol; it could therefore serve as a means of labeling plasma cholesterol for investigating its utilization by the adrenal cortex.2) At rest, about 80% of hydrocortisone derived from plasma cholesterol, the other 20% thus being synthesized in situ from acetate and other unlabeled precursors.3) Under ACTH stimulation the participation of plasma cholesterol in the synthesis of hydrocortisone was the same as at rest; the conversion of plasma cholesterol into hydrocortisone was thus proportional to the production of glucocorticosteroids by the adrenal glands.4) The specific activities of hydrocortisone allowed us to trace its adrenal precursors including adrenal cholesterol. The kinetics of the replacement of adrenal cholesterol by plasma cholesterol underlined the functional heterogeneity of the former. The experimental data were compatible with the following model: A fraction of plasma cholesterol entering the adrenal cell is immediately available for metabolism and conversion into steroid hormones, and another fraction turns over slowly, representing some form of storage.


Experimental infectious canine hepatitis. II. Effect of cortisone acetate on experimental infection in the guinea-pig.


The pressor response to serum and exogenous renin in rats.


Purification of ferritin-conjugated antibody by DEAE-cellulose chromatography.


Simple syndactyly and its surgical repair.


Light and electron microscopic studies of the guinea pig cytomegalovirus.


DNA polymerases from mammalian cells.

DNA polymerases from mammalian cells.


Metabolism of palmitaldehyde-1-14C in the rat brain.


Inosine 5'-phosphate dehydrogenase. Site of inhibition by guanosine 5'-phosphate and of inactivation by 6-chloro- and 6-mercaptopurine ribonucleoside 5'-phosphates.


The heterogeneous distribution of mitochondrial enzymes in normal rat liver.


Solid-phase peptide synthesis of [L-alanine 3-L-isoleucine 5]-angiotensin II.


An electron spin resonance study of copper(II)-peptide complexes in the liquid state.


Structure of the cell wall of Staphylococcus aureus, strain Copenhagen. VII. Mode of action of the bacteriolytic peptidase from Myxobacter and the isolation of intact cell wall polysaccharides.


Selective inhibition of aminoacyl ribonucleic acid synthetases by aminoalkyl adenylates.


A cutaneous syndrome associated with islet-cell carcinoma of the pancreas.


Adnexal skin tumours in Nigeria.

Adnexal skin tumours in Nigeria.


Reduction in renal vascular responses to angiotensin and norepinephrine during carotid sinus stimulation.


The breakdown of embryonic cartilage and bone cultivated in the presence of complement-sufficient antiserum. 2. Biochemical changes and the role of the lysosomal system.


A sero-epidemiological study of adenovirus infections.


Clinicopathologic conference. Weakness, jaundice, and oliguria. Résumé of a case.


Nature of the non-haem iron in ferredoxin and rubredoxin.


Protein-disulphide reductase activity in yeast.


Infection of thymidine kinase-deficient BHK cells with polyoma virus.


Electron spin resonance examination of charge transfer complexes of polycyclic carcinogens and nucleic acid bases.


Comparative cytopathology of rinderpest and bovine parainfluenza 3 viruses in cell cultures.


Herpes simplex virus particles in the nerves of rabbit corneas after epithelial inoculation.


Effect of actinomycin D and halogenated deoxyuridines on the replication of Bittner virus in vitro.


Viral inhibitory activity of polyvinylpyrrolidone.


Absolute configuration of the prostaglandins.


Cell metabolism during melanophore activity.


The Guillain-Barré syndrome associated with acquired cytomegalovirus infection.


Myxovirus dissemination by air.

Myxovirus dissemination by air. Myxoviruses including 150 strains of parainfluenza 1, 15 of parainfluenza 3 and five of influenza B virus were isolated from nasopharyngeal secretions obtained from 300 children less than 3 years of age who developed acute laryngotracheobronchitis during the preceding 48 hours. The patients were examined between October 1966 and January 1967, the peak monthly rate of virus isolation (67%) occurring during January. Parainfluenza 1 virus was isolated from air obtained in the vicinity of one of 30 children whose nasopharyngeal secretions yielded this agent. Samples comprising 150 litres of air were collected for virus assay by placing an Andersen sampler about 60 cm. from the child's face inside an oxygen tent which surrounded the patient. These findings confirm previous observations that parainfluenza 1 virus is the dominant agent associated with acute laryngotracheobronchitis in children in Toronto, and they show that this virus is disseminated in the air.


Observations on thymectomy and carcinogenesis.


Mechanism of action of streptonigrin in leukemic cells.


Utilization of L-methionine and S-adenosyl-L-methionine for methylation of soluble RNA by mouse liver and hepatoma extracts.


Biochemical effects of formycin, an adenosine analog.


The reaction of specific antibody with 2-acetylaminofluorene fixed in liver cells.


Control of lipid metabolism in hepatomas: insensitivity of rate of fatty acid and cholesterol synthesis by mouse hepatoma BW7756 to fasting and to feedback control.


Alkaline ribonuclease and ribonuclease inhibitor in nuclear and nucleolar preparations from normal and neoplastic tissues.


Screening data from the Cancer Chemotherapy National Service Center Screening Laboratories. XLI. Plant extracts.


Inhibitory effects of Sendai virus on Friend virus leukemia in mice.


Genetic studies of carcinogenesis in mice.


Effect of certain drugs on perfused human placenta. VII. Serotonin versus angiotensin-II.


Transient intermediates in the radiolysis of hyaluronic acid.


Cytopathology of Mengovirus infection. I. Relationship between cellular disintegration and virulence.


Cytopathology of Mengovirus infection. II. Proliferation of membranous cisternae.


Granular cell myoblastoma of the breast.


Biophysical properties of poliovirus particles irradiated with ultraviolet light.


The low-temperature luminescent spectra of adenine and its derivatives.


Use of drug combinations for treatment of adeno-virus infections of the eye in tissue culture.


Further advances on the effect of t-fluorodesoxyuridine on adeno-virus infection in tissue culture.


The reaction of reduced cytochrome C oxidase with oxygen.


Ultrastructure of Cocal, Indiana, and New Jersey serotypes of vesicular stomatitis virus.


Granular-cell myoblastoma of the larynx.


A testicular tumor of specialized gonadal stroma.


Adenocarcinoma of the larynx.

Adenocarcinoma of the larynx.


Cryosurgical management of glomus jugulare tumors.


Tumor specific antigens in viral neoplasms and in unrelated virus infected tumors.


Studies with the complement fixing antigen induced by polyoma virus.


SV40 transformation of human diploid cells. A parallel study of viral and karyologic parameters.


Virological studies on chromosome damage of HeLa cells induced by myxoviruses.


Interference with rous sarcoma virus focus formation by a mycoplasmalike factor present in human cell cultures.


Production of interferon by human leukocytes in vitro.


Nonresponsiveness of L cells to the induction of interferon.


Enhancement and inhibition by anionic polymers of plaque development by a variant of Mengo encephalomyelitis virus.


Studies of false positive serological tests for syphilis following smallpox vaccination.


The lipids of human diploid cell strain WI-38.


Destructon of micro quantities of steroids on silica gel as shown by repeated thin-layer chromatography.


Effect of corticosteroid administration on the adrenocorticotropic response to electrical stimulation of the hypothalamus in the dog.


Thermal behaviour of silica and its application to dental investments.  3. The effect of grain size distribution upon the thermal expansion of quartz investments.


Adrenal function in cockerels.

Adrenal function in cockerels.


Determination of iodide clearance and exit rate constants in incubated thyroid lobes.


Neonatal androgen or estrogen treatment and the adrenal cortica response to stress in adult rats.


The behavior of cells before and after virus-induced malignant transformation.


Grand rounds at the Washington University School of Medicine. II. Protoplasts and pyelonephritis.


Androstenedione and its conversion to plasma testosterone in congenital adrenal hyperplasia. The plasma concentration, production rate, and conversion ratio of androstenedione and testosterone were studied in seven children with congenital adrenal hyperplasia (CAH) of the 21-hydroxylase type. Plasma androstenedione and testosterone measured by double isotope derivative assay and estimated blood production rates were manyfold increased in the untreated state, markedly suppressed with glucocorticoid, and increased after the administration of ACTH. The metabolic clearance rate when corrected for body size and the conversion ratio of androstenedione to testosterone were similar to previously determined values in normal adults. Consideration of the androgen concentrations and conversion ratios indicates that in children with CAH, 76% of the plasma testosterone in prepubertal females and 36% in males are derived from peripheral conversion of blood androstenedione. The calculated amount of testosterone unaccounted for by peripheral conversion is similar to normal prepubertal values. This approach indicates that virilization in these children results from increased levels of testosterone but that the major source in CAH of this potent androgen is androstenedione secreted by the adrenal cortex.


Metabolism of vitamin D3-3H in human subjects: distribution in blood, bile, feces, and urine. Vitamin D(3)-(3)H has been administered intravenously to seven normal subjects, three patients with biliary fistulas, and four patients with cirrhosis. Plasma D(3)-(3)H half-times normally ranged from 20 to 30 hours. in vivo evidence that a metabolic transformation of vitamin D occurs was obtained, and a polar biologically active vitamin D metabolite was isolated from plasma. Urinary radioactivity averaged 2.4% of the administered dose for the 48-hour period after infusion, and all the excreted radioactivity represented chemically altered metabolites of vitamin D. The metabolites in urine were mainly water-soluble, with 26% in conjugated form. From 3 to 6% of the injected radioactivity was excreted in the bile of subjects with T-tube drainage and 5% in the feces of patients having no T-tube. The pattern of fecal and biliary radioactivity suggested that the passage of vitamin D and its metabolites from bile into the intestine represents an essential stage for the fecal excretion of vitamin D metabolites in man. Abnormally slow plasma disappearance of vitamin D(3)-(3)H in patients with cirrhosis was associated with a significant decrease in the quantity and rate of glucuronide metabolite excretion in the urine.


Tumors in rhesus monkeys inoculated at birth with homologous cells converted in vitro by Rous sarcoma virus, Schmidt-Ruppin strain.


Ultrastructure of gliomas induced in hamsters with Rous sarcoma virus.


Cerebral gigantism: endocrinological and clinical observations of six patients including a congenital giant, concordant monozygotic twins, and a child who acheived adult gigantic size.


Fatal complications following use of potassium perchlorate in thyrotoxicosis. Report of two cases and a review of the literature.


The Chediak-Higashi syndrome: electrophysiological and electron microscopic observations on the peripheral neuropathy.


Induction of herpes simplex virus lesions on rabbit lip mucosa.


Rapid detection of hyperglycemic rebound in insulinoma surgery.


Cavitary bronchiolar carcinoma with an intracavitary aspergilloma.


NADH-Dependent coupled enzyme assay for urease and other ammonia-producing systems.


Double-blind study of the effect of corticotropin treatment in multiple sclerosis.


A cultural psychiatric study of El-Zar cult in U.A.R.


Partial resolution of the enzymes catalyzing oxidative phosphorylation. 13. Structure and function of submitochondrial particles completely resolved with respect to coupling factor.


On the nature of the changes induced in Escherichia coli by osmotic shock.


The incomplete hydroxylation of individual prolyl residues in collagen.


Stimulation of the catecholamine output of the isolated, perfused adrenal gland of the dog by angiotensin and bradykinin.


Influence of ouabain on the formation of corticosterone in the rat adrenal gland.


Intraperitoneal haemorrhage from primary hepatic carcinoma.


Hypoglycaemia resulting from insulin secreting tumours of the pancreas.


The effect of interferon on SV-40 T antigen production in SV-40-transformed cells.


Observations on the assay of infectious viral ribonucleic acid: effects of DMSO and DEAE-dextran.


Surgical complications of adrenal steroid therapy.


Studies on the metabolism of the protozoa. The molecular structure of the reserve polysaccharide from Astasia ocellata. 1. The flagellate Astasia ocellata synthesizes as reserve carbohydrate a water-insoluble polysaccharide (paramylon) containing chains of beta-(1-->3)-linked d-glucose residues. 2. The average chain length, determined by methylation analysis, is about 43, and the minimum degree of polymerization from periodate oxidation data is 50-55. Most of the molecules are therefore essentially linear.


The biosynthesis of glycerides by mitochondria from rat liver. The requirement for a soluble protein. 1. The synthesis of glycerides from l-3-glycerophosphate and palmitic acid by mitochondrial preparations from rat liver was shown to be stimulated markedly by a soluble factor from the supernatant fraction of the liver. 2. That the soluble factor was a protein was indicated by its inactivation after treatment with papain and after boiling for 3min. at 100 degrees , its precipitation by ammonium sulphate and its behaviour on Sephadex G-200. The soluble factor was purified by ammonium sulphate fractionation and gel filtration. 3. Bovine serum albumin and lipoprotein fractions from rat and human serum also stimulated glyceride biosynthesis but the stimulations were one-twentieth to one-third of that obtained with the soluble factor. 4. The function of the soluble factor could not be explained by assuming a leakage of acyl-CoA synthetase, phosphatidate phosphatase or diglyceride acyltransferase from the mitochondria into the supernatant during preparation of the mitochondrial fraction. 5. Palmitic acid, in the presence of the soluble factor and optimum amounts of ATP and CoA, was a more effective substrate than palmitoyl-CoA or palmitoylcarnitine for the biosynthesis of glycerides by mitochondria.


Degos' acanthoma (acanthome à cellules claires). A report of five cases with particular reference to the histochemistry.


The phosphatidylinositol kinase of rat brain. 1. The presence of a phosphatidylinositol kinase in homogenates of adult rat brain was shown by using labelled ATP or labelled phosphatidylinositol. 2. The kinase was activated by Mg(2+) or Mn(2+) and inhibited by Ca(2+), Cu(2+), K(+), Na(+) and F(-). 3. The detergents sodium deoxycholate, Cutscum and Triton X-100 markedly stimulated the reaction; sodium taurocholate, Tween-20 and cetyltrimethyl-ammonium bromide were less effective. 4. The activity of the enzyme was dependent on SH groups. 5. The subcellular distribution of the kinase in brain resembled that of Na(+)-plus-K(+)-stimulated adenosine triphosphatase and 5'-nucleotidase.


Turban tumour.

Turban tumour.


Herpes simplex encephalitis: two fatal cases. The clinical and pathological features of the first two reported cases of herpes simplex encephalitis occurring in Saskatchewan are presented. The clinical history of an acute onset, an early organic mental syndrome followed by coma, neurologic disturbances, rapid progression and death suggests the diagnosis. The acute, diffuse, inflammatory process with predominant involvement of the temporal lobes of the cerebral hemispheres and the presence of intranuclear inclusions in nerve and glial cells are illustrated. The viral particles were found in electron micrographs from the brain tissue of both patients. The definitive diagnosis was established by the isolation, from postmortem brain tissue, of the herpes simplex virus, which was grown in tissue culture and shown to be pathogenic in suckling mice.


Cushing's syndrome due to an ACTH-producing carcinoma of the thyroid.


Purine N-oxides. XIX. On the oncogenic N-oxide derivatives of guanine and xanthine and a nononcogenic isomer of xanthine N-oxide.


Releasing factors in human hypothalamic and neurohypophysial extracts.


Hypotriglyceridemic effect of corticotropin in man.


Estrogen metabolism in the human. I. Studies in the male using estrone-6,7-3H, with special reference to estrone production rate determinations, the origin of certain urinary estrogen conjugates, and the use of a new mathematical model.


Abnormal diurnal variation of plasma cortisol in patients with renovascular hypertension.


Estrogen metabolism in the human. II. Studies in the male using estradiol-17-beta-6,7-3H.


Recurrent Cushing's syndrome associated with aldosterone deficiency.


The effect of hypophysectomy and corticotrophin on the renal sensitivity to antidiuretic hormone.


Biochemical composition of human testicular fluid collected post mortem.


Purification and properties of poliovirus double-stranded ribonucleic acid.


Prevention of implantation in rats by I.C.I. 46,474: role of histamine.


Preparation and properties of some methylated polycytidylic acids.


What hyperbaric medicine has to offer the industrial physician.


Encephalitis due to herpes simplex in a patient with treated carcinoma of the uterus. Speculations about herpes simplex as a cause of carcinomatous encephalitis.


Effect of potassium on adrenal aldosterone production in rats.


Effects of extrahypothalamic dexamethasone implants on the pituitary-adrenal system.


Inhibition of gastric mucous secretion by ACTH. An experimental study.


Familial cirrhosis with hepatoma.

Familial cirrhosis with hepatoma.


Systems analysis in the study of homeostasis. With special reference to cortisol secretion.


Detection of nanogram quantities of vitamin D by gas-liquid chromatography.


Gas chromatographic analysis of oxidation products of adrenal corticosteroids.


The mechanism of action of UDPG-dehydrogenase.


Isolation and characterization of dinucleoside tetra- and tri-phosphates formed in the presence of lysyl-sRNA synthetase.


Prediction of axillary nodal metastases from the morphology of primary mammary carcinomas. Guide to operative therapy.


Cell transformation by viruses as illustrated by the response of human and hamster renal cells to Simian virus 40.


Primary aldosteronism with unusual secretory pattern.


The tolbutamide tolerance texxt in the evaluation of childhood hypoglycemia.


Intestinal cytochrome response with repair of iron deficiency.


Reduced diphosphopyridine nucleotide oscillations in cell-free extracts from beef heart.


Aromatic acyl phosphates as substrates of acyl phosphatase.


The relation of structural and catalytic protein in the mitochondrial electron transfer chain.


Quantitation of lactate dehydrogenase of developing molar teeth of the mouse.


Inhibition by cortisol of ACTH release from anterior pituitary tissue in vitro.


Response of isolated liver to endotoxin.


Stimulation of adrenal activity by the administration of corticosterone to rats chronically treated with amphenone or methapyrapone.


The infective agent in carp pox.

The infective agent in carp pox.


Lymphographic studies related to the problem of metastatic spread from carcinoma of the female genital tract.


A histochemical investigation of the concept of placental insufficiency.


A Krukenberg tumour of the uterine cervix.


Roentgenography of the diaphragmatic and retrosternal lymphatics by intraperitoneal injection of contrast material.


The challenge to man of the neoplastic cell.


Electron microscopy and classification of the virus of viral haemorrhagic septicaemia of rainbow trout.


Adenovirus endocarditis in mice.

Adenovirus endocarditis in mice. Viral endocarditis developed in 24 percent of 50 newborn mice 6 to 8 days after intraperitoneal inoculation with murine adenovirus. Typical adenovirus intranuclear inclusions were seen in heart-valve lesions, and high titers of virus were recovered from heart tissue. Furthermore, adenovirions were directly visualized by electron microscopy within endothelial cells and fibroblasts of the heart valves.


The effect of pregnancy on the clinical course of sarcoma of the soft somatic tissues.


Studies on nuclear proteins. II. Quantitative distribution of histone fractions in various tissues.


"Free" sialic acid in ovine submaxillary gland.


A rapid technique for distinguishing enzymatically active proteins in the cell"envelope" of Escherichia coli B.


Experimental studies on dust elimination from lungs. V. Effect of sulphuric acid aerosol inhalations on quartz dust elimination from lungs of rabbits in course of a year.


Radiographic appearances in chromolymphadenography.


Repeated infections with respiratory syncytial virus.


Characterization of hamster antibody reacting with papovavirus SV40 tumor antigen.


Adoptive immunity to intracellular infection.


Stabilization of catalase-ammonia complex in frozen solution.


Composition and 32P turnover of phospholipids of rat adipose tissue.


Cytomegalo virus--mononucleosis. Isolation of the virus and demonstration of subclinical infections after fresh blood transfusion in connection with open-heart surgery.


Demonstration of precorticotrophin in blood.


Studies on extracellular phosphatase in Actinomycetes.


Nervous elements in nephroblastoma.

Nervous elements in nephroblastoma.


Reversal of the enzyme profiles of muscle fibres in fast and slow muscles by cross-innervation.


The remote effects of cancer on the nervous system.


Myelinogenesis in the rat brain.

Myelinogenesis in the rat brain.


Herpetic encephalitis.

Herpetic encephalitis.


Acute viral infections of the nervous system.


A fluorometric routine method for urinary unconjugated 11-hydroxycorticosteroids in adrenocortical hypofunction.


An agar cell-suspension plaque assay for isolated viral RNA.


Intratympanic surgical treatment of frey's syndrome.


A study of some acid hydrolases in the liver of the larval and adult lamprey.


ATP formation during enzymatic decomposition of coenzyme B12.


Characteristics of a newborn runt disease induced by neonatal infection with an oncolytic strain of reovirus type 3 (REO3MH). I. Pathological investigations in rats and mice.


Characteristics of a newborn runt disease induced by neonatal infection with an oncolytic strain of reovirus type 3 (REO3MH). II. Immunological aspects of the disease in mice.


Fatal intraperitoneal infection with Clostridium welchii Type A in passively immunised guinea-pigs. The effect on vascular permeability.


Infection by Rous sarcoma virus of various chick embryo tissues cultured on reconstituted collagen membranes.


Granular-cell myoblastoma of the breast.


Salivary tumours: a clinicopathological study and follow-up of 156 cases.


The angiotensin infusion test and peripheral venous renin activity. Forty hypertensive patients were studied to examine the assumption that the angiotensin pressor dose reflects endogenous renin activity. Peripheral renin activity was assayed by the method of Boucher et al.(4) Sensitivity to the infusion of synthetic angiotensin II was determined as suggested by Kaplan and Silah.(1)Sixteen patients with essential hypertension with normal renal angiography required 3.8 ng. angiotensin/kg./min. to raise the diastolic pressure 20 mm. Hg. All but one were sensitive to angiotensin infusion of less than 5 ng./kg./min. Renin activity was normal in all except in one sensitive subject. Angiotensin infusion response and mean renin activity in 13 patients with essential hypertension with abnormal renal angiography were similar to that of the first group. The pressor dose in 11 renovascular hypertensives was 9.8 ng./kg./min. All but three had elevated plasma renin activity.OUR RESULTS SUGGEST THAT: (1) the angiotensin infusion test is suitable for differentiating patients with true renovascular hypertension from those with essential hypertension with or without associated renal artery disease; (2) the angiotensin pressor dose correlates with the level of peripheral venous renin activity (p < 0.01).


Cancer of the lung among nonsmokers. Special reference to histologic patterns.


Crystal bodies in the ultrastructure of multiform glioblastoma.


Semi-automated assays for urinary 17-ketogenic steroids. A comparison of bismuthate and periodate oxidation methods.


Influences of Po2 and pH on resting and active tensions of pulmonary arterial strips.


Correlation of B virus and herpes simplex virus antibodies in human sera.


Buckthorn polyneuropathy.

Buckthorn polyneuropathy.


Effect of thirst and water load on the hypothalamic regulation of the adrenal in the pigeon (Columba livia domestica L.).


Phosphatase activity in the initial phase of healing of skin wounds. Histochemical comparison between the metal-salt and azo-dye methods.


Some metabolic changes during the chick's ontogenesis. A preliminary report.


The detection of hyaluronic acid in the serum and urine of a patient with nephroblastoma.


Pseudohypoparathyroidism 1942-1966. A negative progress report.


Puromycin: a potent metabolic effect independent of protein synthesis.


Inhibition of DNA synthesis in ascites hepatoma cells by normal liver extract.


Virus content of Shope papillomas of cottontail rabbits.


The isolation of aribonucleic acid fraction from rabbit reticulocytes which enhances amino acid incorporation into protein.


Utilization of oxygen by transplanted tumors in vivo.


Glucose consumption by transplanted tumors in vivo.


Relationship between oxygen and glucose consumption by transplanted tumors in vivo.


Common features of the base composition of rapidly labeled RNA of nucleoli in a number of experimental tumors.


Ribonucleic acid components of BAI strain A (myeloblastosis) avian tumor virus.


Muscular subaortic stenosis. The direct relation between the intraventricular pressure difference and the left ventricular ejection time.


Electron microscopy of some human hepatomas.


Succinic thiokinase. IV. Improved method of purification, arsenolysis of guanosine triphosphate, succinate-dependent guanosine triphos- phatase activity, and some other properties of the enzyme.


The dissociation of D-fructose 1,6-diphosphatase by adenosine 5'-phosphate.


Quantitative determination of the ribosomal ribonucleic acid content of liver and Novikoff hepatoma from fed and from fasted rats.


Kinetic studies of dogfish liver glutamate dehydrogenase with diphosphopyridine nucleotide and the effect of added salts.


Structural properties of stellacyanin, a copper mucoprotein from Rhus vernicifera, the Japanese lac tree.


Effect of sodium chloride on the activity of a soluble malate dehydrogenase from pea seeds.


Biosynthesis of cardiolipin in Escherichia coli.


The role of hypothesis testing in clinical trials. Biometrics seminar.


Glomus jugulare tumour with changing drum membrane picture.


Species specificity of tyrosine transfer RNA's from Escherichia coli and yeast.


Histochemistry of the chick esophagus and trachea. II. Enzymes, nucleic acids, proteins, carbohydrates, and fats.


Case records of the Massachusetts General Hospital. Case 28-1967.


The inhibitory effect of chloroquine on rat adipose tissue metabolism in vitro.


Intraperitoneal lipid granuloma.

Intraperitoneal lipid granuloma.


Overproduction of deoxyribonucleic acid as a cause of cell death in cultures infected with canine hepatitis virus.


Placental pathology. V. Placental lesions of cattle experimentally infected with infectious bovine rhinotracheitis virus.


Reactivation of antibody-neutralized foot-and-mouth disease virus by organic chemicals and inhibition by 1-butanol.


Complement-fixing antibodies as a measure of immunity of cattle to the virus of vesicular Stomatitis New Jersey.


Association of myxovirus parainfluenza-3 with pneumoenteritis of calves: virus isolation.


Characteristics of isolated and purified preparations of the outer and inner membranes of mitochondria.


The pathogenesis of encephalitis caused by the infectious bovine rhinotracheitis virus.


Viral respiratory diseases in Nigeria: a serological survey, II. complement fixing antobody levels of adenoviruses, respiratory syncytial virus, psittacosis virus.


Physical and topological properties of circular DNA. Several types of circular DNA molecules are now known. These are classified as single-stranded rings, covalently closed duplex rings, and weakly bonded duplex rings containing an interruption in one or both strands. Single rings are exemplified by the viral DNA from phiX174 bacteriophage. Duplex rings appear to exist in a twisted configuration in neutral salt solutions at room temperature. Examples of such molecules are the DNA's from the papova group of tumor viruses and certain intracellular forms of phiX and lambda-DNA. These DNA's have several common properties which derive from the topological requirement that the winding number in such molecules is invariant. They sediment abnormally rapidly in alkaline (denaturing) solvents because of the topological barrier to unwinding. For the same basic reason these DNA's are thermodynamically more stable than the strand separable DNA's in thermal and alkaline melting experiments. The introduction of one single strand scission has a profound effect on the properties of closed circular duplex DNA's. In neutral solutions a scission appears to generate a swivel in the complementary strand at a site in the helix opposite to the scission. The twists are then released and a slower sedimenting, weakly closed circular duplex is formed. Such circular duplexes exhibit normal melting behavior, and in alkali dissociate to form circular and linear single strands which sediment at different velocities. Weakly closed circular duplexes containing an interruption in each strand are formed by intramolecular cyclization of viral lambda-DNA. A third kind of weakly closed circular duplex is formed by reannealing single strands derived from circularly permuted T2 DNA. These reconstituted duplexes again contain an interruption in each strand though not necessarily regularly spaced with respect to each other.


Oncogenicity of bovine adenovirus type 3 in hamsters.


Increased angiotensin formation in response to carotid occlusion in the dog.


Multiplication of an attenuated RV11 strain (type SAT12) of foot-and-mouth disease virus in sheep.


Electron microscopic studies on lumpy skin disease virus type "Neethling".


The susceptibility of differentiating muscle clones to Rous sarcoma virus.


Soluble collagen proline hydroxylase and its substrates in several animal tissues.


Requirement for the integrity of the viral genome for the induction of host DNA synthesis by polyoma virus.


Spin-labeled hemoglobin.

Spin-labeled hemoglobin.


ESR study of nucleosides and nucleotides bombarded with hydrogen atoms.


Biological half-life of a synthetic corticotrophin in men.


Nucleic acid homology studies of SV 40 virus-transformed and normal hamster cells.


The polycystic ovary. VI. A hilus cell tumour of the ovary associated with polycystic ovarian disease: in vivo and in vitro studies.


Oestrogens in virilizing adrenal carcinoma.


The relationship of parathyroid activity to pyrophosphate excretion.


ACTH induced changes in tryptophan turnover along induceable pathways in man.


Response of pineal sympathetic nerve processes and endings to angiotensin.


Use of neutron activation in the characterization of small quantities of nucleic acids.


Nucleic acid of influenza virus.

Nucleic acid of influenza virus.


Stretchings and yawnings induced by adrenocorticotrophic hormone.


Effect of cervical sympathetic stimulation on accommodation in monkeys. An example of a beta-adrenergic, inhibitory effect.


Effect of prolonged ACTH administration on the gonads of sexually mature chickens.


Metabolic activities of the endolymphatic sac. An enzyme histochemical and autoradiographic study.


Air sampling and analysis with microcolumns of silica gel.


Effects of 4-hydroxypyrazolo(3,4-d)pyrimidine upon the catabolism of purines by various tissues of the rat and upon the rate of growth of Morris 5123-C hepatoma.


A case report of two primary tumours: bronchial carcinoid and adenocarcinoma in the same lung.


Experimental infection of calves with hemadsorbing enteric (HADEN) virus.


Application of x-ray diffraction to the determination of chrysotile in bulk or settled dust samples.


Infection of germfree pigs with a porcine enterovirus.


Morphological changes in cells transformed in vitro by bovine papilloma virus.


In vitro activity of some 2-N3-guanidinobenzimidazole derivatives against poliovirus.


Rhinoviruses isolated from Japanese children with common cold.


Prevention by antidepressant drugs of reserpine effect on pyridine nucleotide metabolism.


A study on the sensitivity test of cancer cells to anticancer drugs.


Effect of angiotensin on renal transport of sodium in renovascular hypertension.


Estrogen influence on pituitary and adrenal function in the rat.


Response of rat ovarian cholesterol to gonadotropins and anterior pituitary hormones.


Antilipolytic effects of sulfonylurea drugs on induced lipolysis in isolated fat cells of the rat.


The osmiophilic particles of astrocytes. Viruses, lipid droplets or products of secretion?


Microchemical and histochemical observations in a case of Krabbe's leukodystrophy.


Cadmium-induced selective lesions of sensory ganglia.


Sudden deafness associated with adenovirus infection.


Delayed diagnosis of pregnancy-associated trophoblastic disease.


Renin in experimental "toxemia of pregnancy".


Plasma and adrenal corticosterone levels following exposure of the two-day-old rat to various stressors.


Immunologic restoration of neonatally thymectomized rats with thoracic duct lymphocytes.


Aerosol stability of three acute respiratory disease viruses.


Incompetent relationship between coxsackievirus A6 ribonucleic acid and mouse cells.


Effect of heat and nucleotides on human erythrocyte inorganic pyrophosphatase.


Comparative viral sensitivity of bovine and avian cells before and after storage in liquid nitrogen.


Relation of renal arterial pressure to activity of renin-angiotensin system in renal hypertension.


Changes in the adrenal glands and other organs of intact and hypophysectomized rats following ACTH and adrenal steroids.


The skin of primates. XXXII. The Philippine tarsier (Tarsius syrichta).


The formation of unpaired electrons on sulphur atoms in irradiated dry proteins as studied by electron spin resonance.


The role of virus in radiation leukemogenesis.


Adhesiveness of cancer cells as revealed by microincineration technique.


Histochemical study on alkaline phosphatase activity in human neoplasms.


Separation and quantitative determination of 32P-labelled lipids from brain particulates by thin-layer chromatography.


Viral oncolysis: increased immunogenicity of host cell antigen associated with influenza virus. A2G mice could be solidly immunized against the Ehrlich ascites tumor by single intraperitoneal injections of homogenized and lyophilized tumor cells which had been infected with oncolytic strains of influenza A virus. Similar homogenates from noninfected tumor cells were not immunogenic, even when mixed with egg-grown virus. The immunizing principle in viral oncolysates could not be separated from the oncolytic virus by differential centrifugation or adsorption to and elution from red cells. It could be inhibited by antibody raised in rabbits against the egg-grown oncolytic virus. This reaction showed serologic specificity. Thus, the immunogenicity of an oncolysate produced with the WSA strain of neurotropic influenza virus could be inhibited by rabbit anti-WSA, but not by rabbit antibody to the TUR strain of fowl plague virus. Conversely, the immunogenicity of an oncolysate prepared with the TUR strain could be inhibited by rabbit anti-TUR, but not by anti-WSA. When mice were preimmunized (primed) with egg-grown WSA virus, their antitumor response to a later injection of WSA oncolysate was of the anamnestic type. Priming with egg-grown influenza B virus had no such effect. It was concluded that the immunogenicity of certain host cell components was greatly increased by incorporation into the makeup of the oncolytic virus.


Improvement of the plaque assay of herpes simplex virus in HeLa cells.


An outbreak of pharyngoconjunctival fever due to adenovirus type 3.


Infection of cattle with parainfluenza 3 virus with special reference to udder infection. I. Virus isolation from milk.


Immunofluorescence of Japanese encephalitis virus infection in vitro: localization of viral antigen in canine cerebellar tissue cultures.


Infection of cattle with parainfluenza 3 virus with special reference to udder infection. II. Pathogenicity of the virus for cattle, with particular reference to the mammary gland.


Effect of thymectomy on transplantation resistance induced by polyoma tumour homografts.


Changes in the EEG of the hypothalamus and limbic system after administration of ACTH, SU-4885 and ACH in rabbits with special reference to neurohumoral feedback regulation of pituitary-adrenal system.


Comparison of the effect of alpha- and gamma-radiation on an enzyme.


A culture method of growing Entamoeba histolytica and other anaerobic amoebae for the study of their nuclear division.


The use of combined hyperbaric oxygen and Polymyxin B in the treatment of Pseudomonas infections of mice.


The degradation of mature collagen: a laboratory study.


The central nervous system pathology in multiple neurofibromatosis.


A generalized herpes virus infection in owl monkeys. REP NO 670.


Selected hematological and biochemical tests performed with blood and serum of adult rainbow trout (Salmo gairdneri) with a high incidence of hepatoma.


The inhibition of streptococci by lactoperoxidase, thiocyanate and hydrogen peroxide. The effect of the inhibitory system on susceptible and resistant strains of group N streptococci. 1. The growth of the lactoperoxidase-sensitive Streptococcus cremoris 972 in a synthetic medium was inhibited by lactoperoxidase and thiocyanate. The glycolysis and oxygen uptake of suspensions of Strep. cremoris 972 in glucose or lactose were also inhibited. The lactoperoxidase-resistant Strep. cremoris 803 was not inhibited under these conditions but was inhibited in the absence of a source of energy. 2. Lactoperoxidase (EC 1.11.1.7), thiocyanate and hydrogen peroxide completely inhibited the hexokinases of non-metabolizing suspensions of both strains. The inhibition was reversible, hexokinase and glycolytic activities of Strep. cremoris 972 being restored by washing the cells free from inhibitor. The aldolase and 6-phosphogluconate-dehydrogenase activities of Strep. cremoris 972 were partially inhibited but several other enzymes were unaffected. 3. The resistance of Strep. cremoris 803 to inhibition was not due to the lack of hydrogen peroxide formation, to the destruction of peroxide, to the inactivation of lactoperoxidase or to the operation of alternative pathways of carbohydrate metabolism. 4. A ;reversal factor', which was partially purified from extracts of Strep. cremoris 803, reversed the inhibition of glycolysis of Strep. cremoris 972. The ;reversal factor' also catalysed the oxidation of NADH(2) in the presence of an intermediate oxidation product of thiocyanate and was therefore termed the NADH(2)-oxidizing enzyme. 5. The NADH(2)-oxidizing enzyme was present in lactoperoxidase-resistant streptococci but was absent from lactoperoxidase-sensitive streptococci.


Substrate activation and inhibition in coenzyme-substrate reactions cyclohexanol oxidation catalysed by liver alcohol dehydrogenase. 1. The activity of liver alcohol dehydrogenase with cyclohexanol and cyclohexanone as substrates was studied, and the initial-rate parameters were determined from measurements at low substrate concentrations. In contrast with aliphatic ketones, cyclohexanone is a fairly good substrate, although less active than aliphatic aldehydes. The Michaelis constant for cyclohexanol is of the same order as that for ethanol, and the maximum rate and Michaelis constant for NAD(+) obtained with cyclohexanol are very similar to those obtained with primary aliphatic alcohols. The data for this substrate at low concentrations are therefore consistent with a compulsory-order mechanism in which ternary complexes are not rate-limiting. 2. With large concentrations of NAD(+), substrate activation is observed with increasing concentrations of cyclohexanol, whereas with small NAD(+) concentrations substrate inhibition is observed. This complex behaviour is explained by a mechanism previously proposed for this enzyme, which also satisfactorily described the kinetics of oxidation of primary and secondary aliphatic alcohols and aldehydes, including the substrate inhibition exhibited by primary alcohols, and the reduction of aldehydes. The activation with large concentrations of both NAD(+) and cyclohexanol is attributed to the formation of an abortive complex, E.NADH.ROH, from which NADH dissociates more rapidly than from the normal product complex E.NADH. Substrate inhibition in the presence of small NAD(+) concentrations is attributed to the formation of an active complex E.ROH, with which NAD(+) reacts more slowly than with the free enzyme. 3. Some support for these mechanisms of substrate activation and inhibition is obtained by approximate theoretical calculations, and their applicability to other two-substrate reactions that exhibit complex initial-rate behaviour, as a more likely alternative to the postulate of a second binding site for the substrate, is suggested.


Glucose metabolism in the mucosa of the small intestine. Glycolysis in subcellular preparations from the cat and rat. 1. Lactic acid formation in supernatant fractions of homogenates of cat or rat small-intestinal mucosa was measured under optimum conditions with glucose, fructose, glucose 6-phosphate, fructose 1,6-diphosphate or 3-phosphoglycerate as substrate. 2. Between 80 and 107% of the glycolytic activity of the homogenate was recovered in these particle-free preparations when glucose, fructose, glucose 6-phosphate or fructose 1,6-diphosphate was used as substrate. 3. Evidence was obtained that hexokinase and phosphofructokinase were the rate-limiting enzymes in the initial sequence of glycolytic reactions. The limitation of rate by hexokinase was much more pronounced in preparations from the cat than in those from the rat. 4. With subcellular preparations from cat or rat small intestine lactic acid was also formed from ribose 5-phosphate and at rates similar to those observed with glucose. 5. A higher rate of glycolysis was observed with glucose 6-phosphate as substrate with preparations from the proximal half of the small intestine of the rat as compared with the distal half. 6. Mucosal preparations from rats starved for 24-48hr. exhibited only about one-quarter of the glycolytic activity of those of fed control groups. The decreased rate of formation of lactic acid from either glucose or fructose was mainly due to a decrease in the activity of hexokinase(s). The activities of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase and a number of other enzymes were not significantly decreased by starvation. 7. The results are discussed in relation to metabolic control of glycolysis in other mammalian tissues.


Interactions of protein-denaturing salts with model amides.


Sphingosine and its lower homologues from bovine heart sphingomyelin fraction.


Studies of nucleotide sequences in tobacco mosaic virus ribonucleic acid. 3. Periodate oxidation and semicarbazone formation.


Studies of nucleotide sequences in tobacco mosaic virus ribonucleic acid. IV. Use of aniline in stepwise degradation.


Biological characterization of a new and highly potent synthetic analogue of corticotrophin.


The adrenocorticotropic action of a new synthetic pentacosapeptide.


Course of hypertension during prolonged treatment with heterologous renin.


The effect of perchlorate ion and some pharmacological agents on the sensitivity of the frog (Rana pipiens) rectus abdominis to acetylcholine.


On the mechanism of potentiation of kinins by inhibitors of the fibrinolytic system.


Effects of angiotensin II and epinephrine infusion on juxtaglomerular cell granularity and arterioles of the rat kidney.


Effect of age, sex, hypophysectomy and gonadectomy on plasma corticosterone levels and adrenal weights following the administration of ACTH and stress.


Antibodies to synthetic corticotrophin.

Antibodies to synthetic corticotrophin.


Oncogenicity of Bryan strain rous sarcoma virus for rodents.


Transfer of metabolic energy to sodium.

Transfer of metabolic energy to sodium.


L-Lysine dehydrogenase deficiency in a patient with congenital lysine intolerance.


Alpha-Glycerophosphate dehydrogenase in mouse adrenal cortex.


Susceptibility of Old World monkeys to Yaba virus.


Interaction of poly-2-vinylpyridine-1-oxide with silicic acid.


The structure and function of the adhesive organ in strigeid trematodes.


Stimulation of DNA synthesis and complement-fixing antigen production by SV40 in human diploid cell cultures: evidence for "abortive" infection.


Acquisition of enzyme function by mouse kidney cells abortively infected with papovavirus SV40.


Protein synthesis related to epidermal differentiation.


Viral antigens in differentiating muscle colonies after infection with Rous sarcoma virus in vitro.


Malignant conversion of cells in vitro by carcinogens and viruses.


Induction of DNA synthesis by SV40.

Induction of DNA synthesis by SV40.


An intermediate in the synthesis of poliovirus RNA.


Laboratory values in severely stunted mental defectives.


Changes in nicotinamide adenine dinucleotide concentration of Bacillus cereus during growth.


Intracellular distribution of vitamin D in rat liver.


Studies on lipoamide dehydrogenase of Bakers' yeast. VI. Molecular weight and some properties.


Calfhood immunization against an experimental Parainfluenza and Pasteurella challenge. An inactivated vaccine including Parainfluenza-3, PASTEURELLA MULTOCIDA and PASTEURELLA HEMOLYTICA stimulated good immunity in calves against an experimental shipping fever challenge. Calves were first vaccinated when 4 to 10 weeks old and revaccinated at weaning age. There were higher HI titers, lower temperatures, and less lung lesions recorded in the vaccinated group when compared to the control animals.


Possible occurrence of cytochromes in isolated thymus nuclei.


Growth inhibition of polyoma-transformed cells by contact with static normal fibroblasts.


Hormonal regulation of plasma free fatty acid concentration in ruminants.


Some nuclear-substituted derivatives of alpha-methyldopa.


1,3,2-diazaphosphorine 2-oxides. 3. Preparation and biological evaluation of some 1,3-bis(aralkyl)-2-halo-1,3,2-diazaphosphorine 2-oxides and related compounds.


Some hydrazine derivatives of (4-biphenyl)glyoxal.


Excision of dimers and hydrates from ultraviolet-irradiated poly U by pancreatic ribonuclease.


Abnormal metabolic response to ultraviolet light of a recombination deficient mutant of Escherichia coli K12.


The removal of the bound ADP of F-actin.


Renin activity of the kidney in the spontaneously hypertensive rat.


Transient pulmonary densities around retained lipiodol.


The influence of the environment on avian leucosis.


Studies on oxidative phosphorylation. XI. Energy-dependent reduction of diphosphophyridine nucleotide by ene-diol compounds in the presence of dyes.


Peptide-associated and antigenic changes accompanying periodic acid oxidation of human plasma orosomucoid.


An intermediate of oxidative phosphorylation from Alcaligenes faecalis.


Bile salts and calcium absorption.

Bile salts and calcium absorption. 1. The study of the effect of bile salts on enhancing calcium absorption in the rachitic chick has been extended to bile salts not present in chick bile, e.g. glycine conjugates and bile alcohol sulphates. 2. Bile and bile salts cause an increase in calcium absorption from sparingly soluble calcium hydrogen phosphate when compared with a suspension of calcium hydrogen phosphate in saline. 3. If the bile ducts of normal rats are tied the absorption of calcium from calcium hydrogen phosphate decreases but can be restored by giving bile salts with the calcium salt. 4. Bile salts increase solubility in water of the sparingly soluble calcium salts, phytate and phosphate at pH values between 6 and 8. 5. Bile salts increase the solubility in lipid solvents of calcium in approximately the same proportion as they increase the absorption of calcium from the gut. 6. The physiological role of bile in calcium absorption and its mode of action are discussed.


Bile and the absorption of strontium and iron. 1. Strontium absorption was studied in vivo with loops of ileum in rachitic chicks and found to be increased by vitamin D(3), bile salts and sodium lauryl sulphate. 2. Bile salts and sodium lauryl sulphate rendered strontium soluble in butanol-benzene (1:1, v/v). 3. Bile was not concerned in the absorption of iron in rats from its water-soluble form, ferrous sulphate. 4. Ligation of the bile ducts in rats caused a decrease in the absorption of iron presented as its sparingly soluble phosphate. 5. The effect of bile on cation absorption is discussed.


The control of rat-heart phosphofructokinase by citrate and other regulators. 1. The effects of ATP, inorganic phosphate and citrate on the relationship between fructose 6-phosphate concentration and initial velocity of reaction has been investigated with a partially purified preparation of rat-heart phosphofructokinase. 2. At low concentrations of ATP (<80mum) rate curves for fructose 6-phosphate approximated to Michaelis-Menten kinetics. At higher ATP concentrations rate curves were sigmoid, the K(m) for fructose 6-phosphate increased and the reaction appeared to be first-order with respect to fructose 6-phosphate at concentrations above its K(m) and of a higher order at concentrations below its K(m). Inorganic phosphate lowered the K(m) for fructose 6-phosphate and the concentration at which the apparent kinetic order decreased. 3. At 40mum-ATP, citrate was an activator at low concentration (<100mum) and an inhibitor at higher concentrations. At 0.5mm-ATP, citrate was inhibitory at all concentrations tested. 4. A new method for phosphofructokinase assay using [U-(14)C]fructose 6-phosphate is described which allows measurements to be made of the velocity of the forward reaction at known concentrations of the products of the reaction. With this method confirmatory evidence has been obtained that concentrations of ATP, AMP, phosphate and citrate may regulate phosphofructokinase in the perfused rat heart.


The inhibition of cationic myotropic drugs by compounds 48/80 and 46/108.


Antimycin-insensitive oxidation of succinate and reduced nicotinamide-adenine dinucleotide in electron-transport particles. I. pH dependency and hydrogen peroxide formation.


Antimycin-insensitive oxidation of succinate and reduced nicotinamide-adenine dinucleotide in electron-transport particles. II. Steroid effects.


Purification of human renin.

Purification of human renin.


Simultaneous measurements of 24na+-efflux and pyridine nucleotides in slices of rat liver.


Regulation of pyruvate dehydrogenase activity in Escherichia coli K12.


Mannitol kinase in cell-free extracts of Escherichia coli.


The presence of D-malate dehydrogenase (D-malate:NAD oxidoreductase) in Serratia marcescens.


Localization of glucosamine-incorporating materials at epithelial surfaces during salivary epithelio-mesenchymal interaction in vitro.


Electron spin resonance study on the free radicals produced from 4-hydroxyaminoquinoline 1-oxide and its significance in carcinogenesis.


Blood corticosterone level of tumor-bearing rats.


Possible transmission of leukemia virus in AKR mice through milk.


Free radical produced by interaction of aromatic hydrocarbons with tissue components.


Survival of host mice and induced resistance to transplantable ascites tumors.


Cellular and humoral aspects of heterotransplantation immunity.


Neoplasms and the vagus.

Neoplasms and the vagus.


Mechanisms of intrarenal hemodynamic changes following acute arterial occlusion.


Sex difference in resting pituitary-adrenal function in the rat.


Relation of fatty acid structure to release and esterification of free fatty acids.


Parainfluenza virus immunization. 3. Antigenicity of aqueous, egg-adapted, parainfluenza types 1 and 2 virus vaccines.


Experimental silicosis. The "atypical" reaction in the Syrian hamster.


Colorimetric assays for serum alanine transaminase and lactic dehydrogenase using diazonium zinc salt.


Massive blood replacement.

Massive blood replacement.


Biosynthesis of mannophosphoinositides by Mycobacterium phlei. The family of dimannophosphoinositides.


A rapid assay for urinary porphyrins by thin-layer chromatography.


The binding of nucleotides and calcium to the extracellular nuclease of Staphylococcus aureus. Studies by gel filtration.


Base sequence at the 5'-linked terminus of tobacco mosaic virus ribonucleic acid.


Studies on the polymer of adenosine diphosphate ribose. I. Enzymic formation from nicotinamide adenine dinuclotide in mammalian nuclei.


Studies on the polymer of adenosine diphosphate ribose. II. Characterization of the polymer.


Adenosine triphosphate conservation in biosynthetic regulation. Escherichia coli phosphoribosylpyrophosphate synthase.


Epiandrosterone excretion in man.

Epiandrosterone excretion in man.


Relationship between the concentration of corticosteroids in avian plasma and nasal gland function.


Reactivation of ether-inactivated infectious bovine rhinotracheitis virus.


Separation of oxytocin from the substances that interfere with its biological assay on the isolated rat mammary gland.


Enzymatic activity of primary isolates of staphylococci in relation to antibiotic resistance and phage type.


Electronic spectra of P2,As2, and Sb2 molecules.


Foot-And-Mouth Disease.

Foot-And-Mouth Disease.


Cardiac effects of angiotensin injections into perfused right coronary artery.


The clinical significance of cytomegalovirus infection in renal transplant recipients.


Ovariectomy and suckling intensity effects on mammary nucleic acid, prolactin, and ACTH.


Wilm's tumour: results of treatment by early post-operative radiotherapy.


Adrenal corticoid levels in human cerebrospinal fluid.


Characteristics of bovine mammillitis virus.


Aerobic metabolism of Streptococcus agalactiae. Streptococcus agalactiae cultures possess an aerobic pathway for glucose oxidation that is strongly inhibited by cyanide. The products of glucose oxidation by aerobically grown cells of S. agalactiae 50 are lactic and acetic acids, acetylmethylcarbinol, and carbon dioxide. Glucose degradation products by aerobically grown cells, as percentage of glucose carbon, were 52 to 61% lactic acid, 20 to 23% acetic acid, 5.5 to 6.5% acetylmethylcarbinol, and 14 to 16% carbon dioxide. There was no evidence for a pentose cycle or a tricarboxylic acid cycle. Crude cell-free extracts of S. agalactiae 50 possessed a strong reduced nicotinamide adenine dinucleotide (NADH(2)) oxidase that is also cyanide-sensitive. Dialysis or ultrafiltration of the crude, cell-free extract resulted in loss of NADH(2) oxidase activity. Oxidase activity was restored to the inactive extract by addition of the ultrafiltrate or by addition of menadione or K(3)Fe(CN)(6). Noncytochrome iron-containing pigments were present in cell-free extracts of S. agalactiae. The possible participation of these pigments in the respiration of S. agalactiae is presently being studied.


An electron microscopy study of phosphatase activity in the endothelial cells of rabbit aorta.


Ultraviolet sensitivity and functional capacity in bacteriophage T4.


Effect of 5-fluorouracil on poliovirus-induced RNA polymerase.


Fiber glass. Chemistry and technology.

Fiber glass. Chemistry and technology.


Anti-viral activity of azathymidine and uracil methyl sulphone.


Electron spin resonance studies of the reactions of nitric oxide and nitrogen dioxide with polymethyl methacrylate.


Complement-fixing antibodies in monkeys bearing tumours induced by Rous sarcoma virus.


Adjuvant principle of pertussis vaccine in the mouse.


Marker studies of poliovirus.

Marker studies of poliovirus.


Spectral changes observed in irradiated glycine.


Comparative study of fowl plague virus and a virus isolated from man.


Primary malignant hepatoma associated with hypoglycaemia. Case report with a review of the literature.


Prostaglandins: members of a new hormonal system. These physiologically very potent compounds of ubiquitous occurrence are formed from essential fatty acids.


Insulin biosynthesis: evidence for a precursor. Human islet cell tumor tissue and isolated islets of Langerhans from rats incorporated radioactive amino acids in vitro into insulin and a larger acid-alcohol soluble protein which could be separated from insulin by gel filtration. The amino acids were incorporated into the larger protein earlier than into insulin; only after incubation of islets for approximately 30 minutes did radioactivity begin to appear in insulin. The transfer of about 70 percent of the radioactivity of the larger protein to insulin was demonstrated in the absence of new peptide bond synthesis (cycloheximide), or during incubation with unlabeled amino acid (chase). The results indicate that the larger protein is a precursor in the biosynthesis of insulin. The name "proinsulin" is suggested for this protein.


Oncogenicity by DNA tumor viruses: enhancement after ultraviolet and cobalt-60 radiations. Simian virus-40, polyoma, and LLE46 virus preparations were treated with ultraviolet or gamma radiations (cobalt-60) in a frozen state. Infectivity and induction of complement-fixing antigen and DNA synthesis declined as a logarithmic function of dose, the latter two properties being more resistant than infectivity to radiation by a factor of 2 to 5. Oncogenicity of all three viruses did not decrease with progressive amounts of both types of irradiation, but actually increased in absolute and relative terms (per infectious unit), even at the maximum dose of irradiation used (24,000 ergs per square millimeter per minute and 2.7 x 10(6) rads).


Effect of neuraminidase on isozymes of alkaline phosphatase and leucine aminopeptidase.


Isolation and culture of chondrocytes from human adult articular cartilage.


Preparation, isolation and properties of [35-S] phenylthiocarbamyl angiotensin.


The effect of traumatic shock on metabolism of ethyl alcohol in mice. 3. The interaction of treatments by nicotinamide, diphosphopyridinenucleotide and traumatic shock on the rate of whole-body metabolism of ethyl alcohol.


Tumour-incidence in progeny of thalidomide-treated mice.


Antigenic composition of transplanted rat hepatomas originally induced by 4-dimethylaminoazobenzene.


Effect of lysolecithin on the oxygen uptake of tumour cells, polymorphonuclear leucocytes, lymphocytes and macrophages in vitro.


Chemical characteristics of microsomal liver specific antigens in the rat.


Response of blood basophils to Rous sarcoma virus infection in chicks and its significance.


Influence of sex hormones on frequency of micturition.


Interfering component of vesicular stomatitis virus.


Tritiated angiotensin: preparation and purification.


Inhibition of neuraminidase by polyanions.


Spermagglutination and spermadsorption due to myxoviruses.


Induction of hypertension in rats, with large and small doses of angiotensin.


Electron microscopy of human pancreatic tumors of islet cell origin.


Psychiatric disorder with intracranial tumors of limbic system.


The effect of mitomycin C on the nucleotide pool of a growing culture of Escherichia coli.


Studies on acid hydrolases. I. A procedure for the preparation of acid deoxyribonuclease and other acid hydrolases.


A study of the kinetics of protein and RNA synthesis induced by foot-and-mouth disease virus.


Thrombocytopenic purpura in patient with autoimmune haemolytic anaemia, successfully treated with mercaptopurine.


Evidence for biochemically different types of vesicles in the hepatic microsomal fraction.


Phosphoesterases of Bacillus subtilis. I. Purification and properties of phosphodiesterases.


Vasopressin-induced ACTH secretion in man.


Adrenal function and serum protein concentrations in a kindred with decreased corticosteroid-binding globulin (CBG) concentration.


Tumors and immunological response in rhesus monkeys born after in utero inoculation with Rous sarcoma virus.


Intracerebral transplantation into rhesus monkeys of cell cultures (NCLP-6) derived from a chemically induced primate hepatocarcinoma.


Epithelial tumors of the rat pancreas.

Epithelial tumors of the rat pancreas.


Colonial growth in agar of cells derived from adenovirus-induced hamster tumors.


Spectroscopic studies of substantia nigra pigment in human subjects.


Renin and acid adenosine triphosphatase as lysosomal enzymes.


Separation of antibody-bound and unbound peptide hormones labelled with iodine-131 by talcum powder and precipitated silica.


Purification of 131-I parathyroid hormone with microfine granules of precipitated silica.


Production of an enzootic-like pneumonia in pigs with "protoplasts" of Haemophilus parainfluenzae.


Increased tumour formation by polyoma virus in the presence of non-oncogenic viruses.


Molybdenum-thiol complexes as models for molybdenum bound in enzymes.


Electron spin resonance of xanthine oxidase substituted with molybdenum-95.


Interference not mediated by interferon.


2-dicyanomethylene-1,3-indanedione: a new electron acceptor. The electron affinity of 2-dicyanomethylene-1,3-indanedione (I) (1.33 ev), as computed from charge-transfer spectral analysis and from polarographic measurements of half-wave reduction potential, shows that the incorporation of the 2-dicyanomethylene moiety in 1,2,3-indanetrione (II) enhances its pi-acid character. The electron spin resonance spectrum of the anion radical of I, generated electrolytically in solution, shows hyperfine structure that is due to two nitrogen atoms and four equivalent hydrogen atoms.


On the solution of quartz and precipitation of dolomite in sea water during photosynthesis and respiration.


Phenindione-induced neuropathy.

Phenindione-induced neuropathy.


Experimental herpes simplex infection in the owl monkey.


Interference with fibrin stabilization by polymyxin B sulfate.


Immunofluorescent studies of virus-induced rabbit papilloma (Shope) in vitro.


Complement-fixation reaction of human neoplastic tissue with sera from hamsters bearing virus-induced tumors.


Erythropoietin-like effect of a polycythemic virus.


Sensitization of the mouse to bradykinin.


Rous sarcoma virus infection of chicken buffy coat macrophage.


A rapid screening method for detection of T antigens in sera of tumor-bearing hamsters.


Effects of prednisolone and triamcinolone on corticosteroid levels in parotid fluid, serum, and urine.


Heterogenous bone graft in orbital reconstruction.


Generalized eruptive histiocytoma. Enzyme histochemistry and electron microscopy.


Clear cell hidradenoma. Histological, histochemical, and electron microscopic studies.


Hypophosphatasia in an adult.

Hypophosphatasia in an adult.


Bacterial endocarditis due to Actinobacillus actinomycetemcomitans.


The isolation of cytomegalovirus from peripheral blood.