A new method for the quantitative determination of monosaccharides, amino sugars and N-acetylneuraminic acid and of 6-deoxyhexose (fucose) in the presence of other sugars. 1. Monosaccharides, amino sugars and N-acetylneuraminic acid were determined by using an original colorimetric assay procedure, based on the detection of formaldehyde released after periodate oxidation. A range of these compounds was investigated by this method and they were all found to obey Beer's law within the concentration range 0-0.6mumole/ml. 2. A simple method for the determination of 6-deoxyhexose concentration in the presence of other monosaccharides is also described. 3. The optimum pH for the release of formaldehyde from sugars by periodate oxidation was 7.0-7.5. 4. The methods described have considerable advantages over existing assay systems and their particlar value in automatic colorimetry, where the use of concentrated acids is undesirable, is discussed.


The effect of infection with fowl-plague virus on protein synthesis in chick-embryo cells.


Ribonucleic acid synthesis in fowl-plague virus-infected chick-embryo cells.


Enzymatic hydrolysis of phosphosphingolipids by Clostridium perfringens phospholipase C.


The incorporation of [14C3]glycerol into lipids by dispersed bovine mammary cells.


Fluorescence and protein structure. XIV. Tyrosine fluorescence in helical muscle proteins.


Probe of subunit structure in fungal p-diphenol oxidase by treatment with guanidine hydrochloride and at high pH.


Isolation of collagens of high hydroxyproline, hydroxylsine and carbohydrate content from muscle layer of Ascaris lumbricoides and pig kidney.


The inhibitory action of noradrenaline and adrenaline on acetylcholine output by guinea-pig ileum longitudinal muscle strip. 1. Noradrenaline and adrenaline reduce the output of acetylcholine by the guinea-pig ileum longitudinal strip by up to 80%, both in resting conditions and after stimulation. The effect is graded with dose, and is detectable with noradrenaline 2 x 10(-7) g/ml. Adrenaline is approximately 4 times as active as noradrenaline, and its action after being washed out is more persistent.2. If resting output is high, both amines have a proportionately greater effect and their action, as dosage is increased, is to reduce resting output to a basal level, relatively constant from strip to strip, of about 10 ng/g/min.3. With stimulation, the effect of the amine is greater at low frequencies, when the output per volley is high, than at high frequencies. The effect is reduced by increasing the number of shocks delivered. There thus appears to be a basal output per volley, of the order of 1-2 ng/g/volley, which can be reached either by relatively rapid stimulation, by prolonged stimulation, or by treatment with these amines.4. If noradrenaline is applied during continued stimulation at 40/min, the depression of acetylcholine output during its presence is followed by an augmented output when the drug is withdrawn. The magnitude of this "overshoot" increases with the duration of noradrenaline exposure.5. Phenylephrine 4 mug/ml. and amphetamine 20 mug/ml. reduced the acetylcholine output, but isoprenaline 1 mug/ml., dopamine 1 mug/ml. and methoxamine 10 mug/ml. were ineffective.6. Phenoxybenzamine reduced the resting output and increased the stimulation output. Of the two other blocking agents examined, phentolamine had no effect on either resting or stimulation output and ergotamine transiently reduced stimulation output. The effect of phenoxybenzamine was not due to a reaction with either adrenoceptive or muscarinic receptors.7. Phenoxybenzamine, phentolamine and ergotamine abolished the effect of adrenaline and noradrenaline on both resting output and on output in response to stimulation.8. In strips obtained from animals treated with reserpine and guanethidine, a rise in resting acetylcholine output and in stimulation output at low frequencies was found. In these conditions, noradrenaline was still effective.9. Reducing the hydroxytryptamine content of the strips by treatment with p-chloro-(+/-)-phenylalanine did not significantly affect acetylcholine output.10. It is concluded that acetylcholine output by the nervous networks of the longitudinal strip is under the normal control of the sympathetic by a species of presynaptic inhibition mediated by alpha receptors. This implies that for a tissue under dual autonomic control, withdrawal of sympathetic control will lead to a parasympathetic response which is not only unopposed but also itself enhanced.


Inhibition by lidoflazine of the contractile response of the rat isolated colon to angiotensin. 1. In the rat isolated ascending colon, lidoflazine in a concentration of 5 x 10(-7)M slightly enhanced the response to angiotensin, but in a concentration of 10(-6)M it was a strong and unsurmountable antagonist.2. Exposure to lidoflazine (10(-6)M) also inhibited the contractile responses to acetylcholine, 5-hydroxytryptamine and barium ions. The antagonism of acetylcholine and 5-hydroxytryptamine was unsurmountable and quantitatively similar to that of angiotensin. The antagonism of barium ions was much weaker and probably competitive.3. The inhibitory effect of papaverine (10(-5)M) showed a pattern similar to that found for lidoflazine.4. It is concluded that the antagonism of angiotensin by lidoflazine in the rat colon is non-specific.


Preparation and evaluation of standardized amoeba antigen from axenic cultures of Entamoeba histolytica. The successful mass cultivation of Entamoeba histolytica strains in pure culture has made it possible to produce pure amoeba antigens, which should improve the prospects for immunodiagnostic research and simplify the interpretation of the results obtained in tests for amoeba antibodies.The authors have mass-produced immunodiagnostic antigens for amoebiasis serology from 2 axenic strains of E. histolytica and standardized them as dry powders, which upon reconstitution contained approximately 1.8 mg N/ml, or roughly the equivalent of 10 x 10(6) amoebae per ml.The antigens were evaluated with 121 sera from subjects in Costa Rica, South Africa, Taiwan, Thailand and the USA, 49 of the sera being from cases of amoebic liver abscess, 41 from symptomatic intestinal amoebiasis, 19 from asymptomatic intestinal amoebiasis, and 12 from subjects presumed not to have amoebiasis.Positive results with the indirect haemagglutination test were obtained with 100% of amoebic liver abscess sera, 90.2% of those from symptomatic intestinal amoebiasis, 57.9% of those from asymptomatic intestinal amoebiasis, and 16.7% of the normal sera. Positive complement-fixation tests for the respective groups were obtained in 83.8%, 63.3%, 10.5% and 0%, and positive agar-gel diffusion in 79.6%, 53.7%, 0% and 0%.These results compare favourably with earlier reports of similar studies in which non-axenic cultures were used and agree well with those of investigators in different parts of the world to whom samples of the axenic antigens were sent.The authors conclude that the axenic antigens from either strain used, or from the 2 strains pooled, are of broad usefulness in the detection of amoebic antibodies, but point out that the limitations of the tests now used in amoeba serology are not yet clearly understood and that the pattern of antibody persistence after infection requires further study.


Post-tetanic and drug-induced repetitive firing in the soleus muscle of the cat. 1. The effects of a high frequency indirect tetanus on the responses to subsequent infrequently applied nerve shocks have been compared in the tibialis anterior and soleus muscles of cats and rabbits.2. Post-tetanic augmentation of twitches in the cat soleus muscle was shown to be partly due to repetitive firing and partly due to increased synchronization of the muscle fibre response. Post-tetanic repetitive firing was not evident in the responses of the other three muscles studied.3. Post-tetanic repetitive responses in the cat soleus muscle and nerve did not originate in the nerve trunk and were not produced by direct muscle stimulation; they were abolished by doses of tubocurarine smaller than those necessary to reduce the twitch tension below the pre-tetanic level. These findings support the conclusion of others that the repetitive firing originates at the neuromuscular junction.4. The repetitive firing could not be explained by an increase in the sensitivity of the motor endplates to acetylcholine, suggesting that an increase in and/or a prolongation of the output of transmitter from the motor nerve contributes to it.5. The cat soleus muscle was shown to be more sensitive to neostigmine than were the other three muscles studied, and acetylcholinesterase determinations showed that this muscle possesses less enzyme activity.6. It is concluded that an increase in transmitter output, coupled with a weaker cholinesterase activity, probably accounts for the post-tetanic repetitive activity in the cat soleus muscle.7. Post-tetanic repetitive firing was absent in cat soleus muscles which had been cross-innervated with the nerve formerly innervating a fast-contracting muscle.


Radioimmunoassay of ACTH and growth hormone.


Interrelationships between parasites and their hosts. IV. Cytochrome c oxidase thermal-acclimation patterns in a larval trematode and its host.


Enzyme histochemistry of the adult liver fluke, Fasciola hepatica.


Separation of polyphosphates by anion exchange thin layer chromatography.


The use of silver nitrate impregnated silica gel layers in the separation of monoterpene hydrocarbons.


Immunochemical studies of foot-and-mouth disease. V. Antigenic variants of virus demonstrated by immunodiffusion analyses with 19S but not 7S antibodies. Three antigenic variants of foot-and-mouth disease virus, type A, strain 119, were demonstrated in Ouchterlony analyses utilizing serum collected from guinea pigs 7 days postinfection (DPI). Such antisera contain antibodies of the 19S class. Guinea pig antisera that contained antibodies of the 7S class were unable to distinguish between the antigenic variants. Similarly, 19S antibody was able to demonstrate antigenic differences in trypsin- and chymotrypsin-treated viruses that were not detected by 7S antibody-containing antisera. One of the antigenic variants of virus is apparently the wild type and is tentatively considered to have two antigenic determinant groupings termed the a- and b-sites (140S-ab). The 140S-ab variant was the sole or predominant antigenic type produced in guinea pigs and in large plaque-forming- and tissue culture-low passage sources of the virus. Another antigenic variant appears to possess only the b-site (140S-b) and was the major constituent in tissue culture-high passage virus preparations. The third variant, a small plaque former, was also devoid of the a-site and contains an antigenic determinant that is related to, but not identical with, the b-site. This variant appears to be a minor constituent of tissue culture-high passage virus. 7-DPI serum could be absorbed with a suitable concentration of tissue culture-high passage virus to remove antibody reactive with the b-determinant site. This absorbed serum still precipitated 140S-ab virus by virtue of still containing antibody reactive with the a-determinant site; however, the neutralizing activity was eliminated. This suggests that the b-site is critical with respect to neutralization while the a-site is noncritical.


Effect of pH on the protective action of interferon in L cells. The pH of the solution in which interferon was applied to L cells determined the level of resistance developed against challenge with vesicular stomatitis virus (VSV). No inhibition of challenge virus was observed when interferon was applied to cells at pH 6.0. At pH 6.5, partial inhibition of VSV replication was observed and inhibition was maximum at pH 7.0. Evidence was obtained that interferon interacted with L cells at pH 6.0, but that resistance did not develop until the cells were placed in a medium at pH 7.0. These effects were explained by data showing that exposure of cells to a medium at pH 6.0 reversibly inhibited both ribonucleic acid and protein synthesis.


Kinetics of viral deoxyribonucleic acid, protein, and infectious particle production and alterations in host macromolecular syntheses in equine abortion (herpes) virus-infected cells. Infection of exponential-phase suspension cultures of mouse fibroblast cells (L-M) with equine abortion virus (EAV) resulted in inhibition of cell growth and marked alterations in host metabolic processes. The synthesis of deoxyribonucleic acid (DNA) and ribonucleic acid was inhibited within 4 hr after infection and was suppressed by more than 90% by the time of maximal virus replication (14 to 18 hr). The overall rate of protein synthesis, however, was similar in uninfected and virus-producing cells as determined by measurements of net protein and isotope incorporation. The time course of viral DNA and protein synthesis and assembly into mature virus was determined with the inhibitors 5-fluorodeoxyuridine (FUdR) and cycloheximide, respectively. Thus, viral DNA synthesis was essentially completed at 14 hr, and viral protein and infectious virus synthesis was completed at 18 hr. Although the number of plaque-forming units (PFU) produced by FUdR-treated cells (10(3) to 10(4) PFU/ml) was at least 3 logs less than that produced by untreated cells, the yield of physical particles (as determined by electron microscopy) was approximately the same at 30 hr after infection. Besides being relatively non-infective, the particles produced in FUdR-treated cells appeared morphologically incomplete as they contained little or no nucleoid material.


Effect of anticellular serum on the attachment of enteroviruses to HeLa cells. Anticellular serum (ACS), in the absence of an active complement system, was shown to inhibit the attachment of poliovirus types 1 and 2, echovirus type 6, and coxsackievirus types A13, B1, and B3 to viral receptors of live HeLa cells. This is the first report to provide evidence that ACS has an inhibitory effect on the interaction between host cells and coxsackieviruses of group B. The titer of inhibitory activity of ACS varied inversely with the cell concentration used, the reaction being virtually completed after an incubation period of 30 min at 37 C. The inhibitory activity of ACS persisted for more than 4 hr at 37 C, and was shown to be reversible at pH 2.0, revealing that although the receptors for attaching virus were inactivated by ACS the inactivation was not permanent. These findings are consistent with the concept that antibodies in the ACS combine with and blockade viral receptors located at the cell surface. An antiserum with a specificity for inhibiting attachment of coxsackievirus B1 was obtained by dual absorption of ACS with cells saturated with coxsackievirus type B3 and chymotrypsin-treated cells. These findings offer an approach whereby the antigenic relationship of viral receptors to other constituents of the cell surface can be studied.


Cellular origin of a mouse leukemia viral ribonucleic acid. Mouse erythroblastosis virus, a member of the mouse leukemia virus group, was obtained from chronically infected C(3)H mouse embryo cells and purified on sucrose gradients. The ribonucleic acid (RNA) extracted from ribonuclease-treated virus consisted of a rapidly sedimenting (72S) species and a more slowly sedimenting component (4 to 30S). The 72S RNA did not contain base sequences homologous to deoxyribonucleic acid (DNA) from infected cells as determined by hybridization studies. In contrast, the slowly sedimenting RNA enclosed within the virus had base sequences homologous to DNA from infected and uninfected C(3)H mouse embryo cells.


Isolation of defective lysogens from Simian virus 40-transformed mouse kidney cultures. Rescue of simian virus 40 (SV40) from hamster and murine cell lines transformed by nonirradiated or by ultraviolet (UV)-irradiated SV40 (10(-3) to 10(-5) survival) was studied. A combination of tests was employed to detect induction of SV40 synthesis: (i) co-cultivation with susceptible monkey kidney (CV-1) cells; (ii) treating mixtures of transformed and CV-1 cells with UV-irradiated Sendai virus (UV-Sendai) prior to co-cultivation; and (iii) plating untreated or UV-Sendai-treated mixtures of transformed and CV-1 cells with freshly trypsinized CV-1 cells. The first and second tests provided a measure of the total infectious SV40 yield per culture, and the third test provided a measure of the frequency of induction (fraction of transformed cells giving rise to infectious centers). With the combination of tests, SV40 was rescued in all trials from TSV-5 hamster cells, mKS-BU100 mouse cells, and from several lines of mouse kidney cells transformed by UV-irradiated SV40 (mKS-U lines). The frequency of induction was about 7 x 10(-2) for TSV-5 cells, about 3 x 10(-3) for mKS-BU100 cells, greater than 10(-4) for the mKS-U lines which were "good" yielders, and about 10(-5) to 10(-4) for the mKS-U lines which were "average" yielders. SV40 of a plaque type different from parental virus was rescued from four of the mKS-U cell lines. Virus was also easily rescued from: (i) tumor cells produced from the mKS-A line of transformed mouse kidney cells; (ii) mouse kidney cells transformed by SV40 which had been rescued from mKS-BU100 cells; and (iii) tumor cells (HATS) which had been produced by inoculating newborn hamsters with SV40 rescued from mKS-BU100 cells. The frequency of induction of HATS cells was of the same order of magnitude as the frequency of induction of TSV-5 cells. In a study of the kinetics of virus induction, it was shown that SV40 could be detected 28, 40, and 48.5 hr after UV-Sendai treatment of mixtures of CV-1 and TSV-5, HATS, or mKS-BU100 cells, respectively. Although all of the mKS-U lines contained the SV40-specific tumor antigen, some were poor virus yielders (SV40 was recovered in less than 50% of the trials) and five lines were rare virus yielders (SV40 recovered only once in four or more trials). Forty-eight mKS-U lines were nonyielders; SV40 was never recovered by any test used thus far. UV-Sendai-treated mixtures of pairs of nonyielder mKS-U lines with CV-1 cells also did not yield infectious virus. Various factors affecting rescue have been discussed. The mKS-U lines which were poor virus yielders, rare yielders, or which never yielded virus have been classified tentatively as "defective lysogens" which contain mutational lesions at loci essential for detachment of SV40 from integration sites or for SV40 replication, or for both.


Interferon production in mice by vesicular stomatitis virus.


Characterization of intracellular ribonucleic acid specific for the murine sarcoma-leukemia virus complex. Virus particles were continuously produced by a cell line (78A1) of rat embryo fibroblasts that had been transformed by the murine sarcoma-leukemia virus complex. Since most of the mature virions were found in the extracellular fluid and were not cell-associated, a measurable quantity of viral ribonucleic acid (RNA) could not be extracted from these cells. Cycloheximide, a protein inhibitor, was successfully used to accumulate viral RNA within the cells. This ribonuclease-sensitive RNA, with a sedimentation coefficient of 71S, had the same base composition as the high molecular weight RNA (S(20,w) = 71) isolated from purified virions released by the transformed cells.


Viral agents in hepatitis. A review.

Viral agents in hepatitis. A review.


Conformational similarities of vitamin D and cholesterol as enzyme substrates.


Effects of exogenous adrenocorticotropic hormone on palatogenesis in A-Jax mice.


Mode of action of morphine-like drugs on autonomic neuro-effectors.


Synthesis and activity of RNA polymerase of a temperature sensitive poliovirus mutant at an elevated temperature.


Transformation of human blood lymphocytes under the influence of smallpox antigen.


Trisomy 13-15.

Trisomy 13-15.


The superior and inferior mesenteric veins as collateral channels in inferior vena cava obstruction.


Relaxation spectrometry of biological systems.


Changes of plasma renin in the adrenalectomized dog under different conditions.


Comparative NADH-diaphorase content of isoniazid-resistant and isoniazid-susceptible Mycobacterium tuberculosis.


Hyponatraemia and renovascular hypertension. Case report with plasma renin and vascular sensitivity studies.


Identification of the incubation product of Boucher's renin activity assay, by means of radioimmunoassays for angiotensin I and angiotensin II, and a converting enzyme preparation from lung tissue.


Synthesis of N-acetylated deoxyribonucleoside 5'-triphosphates and their utilization in enzymatic formation of single-stranded polydeoxyribonucleotides.


The properties of molecular fragments obtained on treating calfskin collagen with collagenase from Clostridium histolyticum.


Molecular fragments of rat skin collagen obtained on collagenase digestion.


The role of glucose, glucagon and glucocorticoids in the regulation of liver glycogen synthesis.


The interconversion of liver glycogen synthetase a and b in vitro.


The biochemical morphology and morphogenesis of hepatomas.


Effects of ethanol infusion on the redox state and metabolite levels in rat liver in vivo.


Activity of metronidazole against experimental tetanus and gas gangrene.


Cyanide metabolism in higher plants. 3. The biosynthesis of beta-cyanolanine.


Studies on the mechanism of fatty acid synthesis. XXI. The role of fructose 1,6-diphosphate in the stimulation of the fatty acid synthetase from pigeon liver.


Regulation of beta-galactosidase synthesis in Escherichia coli by cyclic adenosine 3',5'-monophosphate.


Synthesis of triacetic acid lactone by the pigeon liver fatty acid synthetase complex.


The pyruvate-phosphate dikinase reaction. The fate of phosphate and the equilibrium.


The nature of disaccharidases in human leukocytes.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 5-1969.


Studies on the RNA from avian myeloblastosis virus.


Chemical composition of the parainfluenza virus SV5.


Evidence for the possible existence of two envelope antigenic determinants and corresponding cell receptors for avian tumor viruses.


Dimethyl sulfoxide enhancement of transformation by polyoma virus.


Age-dependent changes in the level of lysosomal enzymes in the lymphatic organs of the guinea pig.


Surgical problems in hemipelvectomy.

Surgical problems in hemipelvectomy.


Haemolytic-uraemic syndrome: survival after prolonged oliguria.


Effect of non-pressor doses of synthetic B-angiotensin II. in adrenocortical insufficiency.


Alteration of the microwave saturation behaviour after heat treatment in electron spin resonance studies of free radicals produced in DL-valine by x-irradiation.


Fetal infections.

Fetal infections.


Nicotinamide deficiency and adrenocortical steroids.


Mesenteric nerve stimulation by intracarotid administration of angiotensin.


Increased activity in sympathetic nerves induced by angiotensin.


Hepatic extraction of angiotensin after carbon tetrachloride intoxication.


Role of the liver in systemic puridine nucleotide metabolism.


Mechanism of the musculotropic activity of angiotensin on the isolated guinea-pig seminal vsecile.


Effects of some local anaesthetics on abnormalities of neuromuscular transmission produced by neostigamine in rats.


On the bias of the distribution of the 2'-O-methylribonucleotide constituents of yeast transfer RNA.


Effects of silica dust on some erythrocyte enzymes.


Electron microscopic study of the tertiary structure of covalently closed polyoma virus DNA.


Low molecular weight RNA of the chromatin fraction from Novikoff hepatoma and rat liver nuclei.


Transcription of the tryptophan operon of Escherichia coli in vitro. I. Detection and quantitative determination of specific RNA.


The enzymic synthesis of adenylyl-(3'-5')-cytidine.


Biosynthesis of tyrocidine by a cell-free enzyme system of Bacillus brevis ATCC 8185. II. Amino acid substitution in tyrocidine.


Loganin from Mytragyna parvifolia Korth.


The mode of formation of intracytoplasmic structures in tumours induced by viruses of the avian sarcoma leucosis group.


Growth characteristics of axenic strains of Entamoeba histolytica Schaudinn, 1903.


Subcellular distribution of enzymes in Ochromonas malhamensis.


O2-polarographic studies on soluble and mitochondrial enzymes of Crithidia fasciculata; glycerophosphage enzymes.


Experimental induction of various kinds of tumors in ICR mice with cigarette tar.


Effect of angiotensin II on urinary sodium excretion in normal subjects and in cirrhotic patients.


The pharmacological actions of some guanidine esters and their relationship to tetrodotoxin.


Synacthen depot.

Synacthen depot.


Japanese encephalitis studies in Vellore, South India. II. Antibody response of patients.


Japanese encephalitis studies in Vellore, South India. 3. Neutralizing activity of human survey sera.


Japanese encephalitis studies in Vellore, South India. IV. Search for virological and serological evidence of infection in animals other than man.


Hepatoma in childhood. Report of two cases.


Treatment of malignant brain tumors by local methotrexate. A preliminary report.


Growth hormone secretion during nocturnal sleep in normal subjects.


Cushing's syndrome induced by the ectopic production of ACTH by a bronchial carcinoid.


Juvenile nasopharyngeal angiofibroma. Awareness in anesthesia of the juvenile nasopharyngeal angiofibroma.


Plasma 11-deoxycorticosteroid and ACTH response to metyrapone (plasma metyrapone test).


Intestinal protozoa of the baboon Papio doguera Pucheran, 1856.


Virus-induced diabetes in mice.

Virus-induced diabetes in mice.


Grand Rounds. Infantile cytomegalic inclusion disease vs. chronic gastrointestinal disturbances.


The management of multiple sclerosis. II. Serial neurologic examinations and use of corticotropin and glucocorticoids.


Skeletal renewal and metabolic bone disease.


Effect of prlonged ACTH treatment on adrenal circulation in rats.


Bilateral effects of skin stimulation on thalamic neurons.


Prostaglandins: enzymatic analysis.

Prostaglandins: enzymatic analysis. By, means of al specific nicotinamide-adeninie dinucleotide-dependent prostaglanidin dehydrogenase from swine lung, an enizymatic method has been developed for the assay of prostaglandins. The method permits analysis with a lower limit of 10(-12) mole of prostaglandin.


Metronidazole in chronic intestinal amoebiasis.


Sensitivity of microorganisms to antibiotics produced by them.


Morphological and physiological investigations on the action of polymyxin B on Escherichia coli.


Enzymic properties of a mutant of Escherichia coli K12 lacking nitrate reductase.


Vitamin E-deficiency in the guinea pig: effect of succinate on mitochondrial nad-linked oxidation.


Free silica (quartz) analysis by x-ray diffraction utilizing the pellet technique.


The effect of nitrogen mustard on anaphylaxis in the pertussis-vaccinated mouse.


Characterization and solubility of metals associated with asbestos fibers.


Ethyl silicate (tetraethyl orthosilicate, tetraethoxy silane) (C2H5)4-SiO4.


The tarsal tunnel syndrome. Diagnosis and treatment.


Public health problems relating to the Vietnam returnee.


"Induction" of viral antigen in established cell line (SP-8) derived from Shope virus-induced cutaneous papilloma of rabbits.


Prevalence of reticuloendotheliosis in chickens: immunofluorescence studies.


Effect of statolon on acute and persistent murine leukemia and sarcoma virus infections.


Experimental infection of rhesus with simian virus 40 (SV40).


A comparison of the efficacy of endogenous, exogenous, and combined endogenous-exogenous interferon in the treatment of mice infected with encephalomyocarditis virus.


A rapid in vivo assay for SV40 tumor immunity in hamsters.


Plasma 17-hydroxycorticosteroid response to ACTH in M. mulatta: dose, age, weight, and sex.


Characterization of a cytomegalo-like virus isolated from spontaneously degenerated equine kidney cell culture.


The effects of insulin on lipolysis evoked by cyclic AMP and its dibutyryl analog.


Ovarian tumours in children. Report of a rare case, a thecoma in a 12-year-old girl.


Survival rates in peripheral bronchogenic carcinomas up to four centimeters in diameter presenting as solitary pulmonary nodules.


Glucagon stimulation of adenyl cyclase activity of cardiac muscle.


In vitro passages of hamster tumour cells induced with Schmidt-Ruppin strain of Rous sarcoma viruse: their oncogenicity, morphology and karyology.


Estrogens in the menopause and postmenopause.


In vivo study of the inflammatory basophilic responses in mucosal colitis and transmural colitis.


The isolation of nucleic acids from gram-positive bacteria.


Stereochemical studies on the microquantitative detection of vitamin D by gas-liquid chromatography.


Reaction mechanism and stereospecificity of 20 beta-hydroxysteroid dehydrogenase.


On the fragmentation of mitochondria by diethylstilbesterol. II. On the relation of the released proteins to the mitochondrial membranes.


Synacthen depot used in general practice.


Virus infections and respiratory disease of childhood.


Time-course of enzyme adaptation. I. Effects of substituting dietary glucose and fructose at constant concentrations of dietary protein.


Comparative mitochondrial function in two strains of Aspergillus niger.


Isolation and characterization of antigenic components of rat glomerular basement membrane.


Biochemical identification and characterization of the multiple forms of alkaline phosphatase in the developing duodenum of the mouse.


Skeletal maturation.

Skeletal maturation.


Cancer-customs, habits and usages and environment.


The riddles of cancer cells.

The riddles of cancer cells.


Pathology of primary malignant neoplasms of the kidney.


Malignant tumours of kidney. Clinical presentation.


Malignant tumours of the kidney: diagnosis & surgical management.


Radiation treatment in renal cancer: a review.


An analysis of the lumbosacral dermatomes in man.


Fibrous xanthoma of synovium (giant-cell tumor of tendon sheath, pigmented nodular synovitis). A study of one hundred and eighteen cases.


Acute respiratory disease.

Acute respiratory disease.


Derangement of regional cerebral blood flow and of its regulatory mechanisms in acute cerebrovascular lesions.


Working together. 3.

Working together. 3.


Role of tissue histamine in reparative processes.


Preferred centripetal conduction of dendritic spikes in alligator Purkinje cells. Dendritic action potentials in alligator Purkinje cells tend to have a unidirectional preference which favors centripetal over centrifugal propagation. This unidirectional tendency funnels the peripherally evoked dendritic spikes into the lower dendrites and soma of these cells, and it allows the peripheral dendritic branches to operate to a certain extent as partially independent functional units.


Pituitary-adrenal influences on fear responding. In a passive avoidance situation, hypophysectomized male rats show less fear than normal rats, whereas adrenalectomized rats show greater fear than normals. These results probably occur because hypophysectomized rats lack adrenocorticotrophic hormone, which increases arousal or emotionality, whereas adrenalectomized animals lack certain adrenal steroids, which inhibit excitatory effects. The results indicate that adrenocorticotrophic hormone and certain adrenal steroids have opposite effects in regulating fear-motivated behavior.


Pituitary hormones: international colloquium.


Acute cerebellar ataxia associated with herpes simplex virus infection.


A technique for the enzymatic diagnosis of glycogen storage disease on very small tissue specimens.


Contributions of EPR spectroscopy to our knowledge of oxidative enzymes.


Enzymology of the nucleus.

Enzymology of the nucleus.


Extrinsic cotton effects and the mechanism of enzyme action.


Control of aldosterone secretion by the renin-angiotensin system and by corticotropin.


Care in the balance.

Care in the balance.


"Care in the balance".

"Care in the balance".


Nucleic acids and protein synthesis in cancer cell mitochondria. I. Nucleic acids in rat hepatoma mitochondria.


Cassava as food in Nigeria.

Cassava as food in Nigeria.


Anterior meningeal branch of the vertebral artery.


Hepatoma presenting as extrahepatic biliary obstruction. Report of a case.


Two new candidate enterovirus serotypes, isolated from CSF.


The virus watch program: a continuing surveillance of viral infections in metropolitan New York families. VI. Observations of adenovirus infections: virus excretion patterns, antibody response, efficiency of surveillance, patterns of infections, and relation to illness.


The titration of rhinoviruses and of antibodies against them by means of the photometric hemaggregation-inhibition test.


Chromatographic separation of vitamins D2 and D3 and related compounds.


Chromatography of vitamin A compounds on silicic acid columns.


Determination of nucleoside composition of ribonucleic acid by gas-liquid chromatography.


Chemical and ionization interferences in the atomic absorption spectrophotometric measurement of sodium, potassium, rubidium, and cesium.


A method for colorimetric assay of inositol and some of its phosphate derivatives.


Determination of nucleoside composition of ribonucleic acid by thin-layer chromatography.


Rapid spectrophotometric assay for nicotinic acid.


Electrophoresis of peptides on thin layers of silica gel.


A polynucleotide ligase from polyoma virus-infected mouse cells.


Immunological evidence for the absence of a polyoma virus-specific deoxyribonucleic acid polymerase.


The localization of the neutral glycosphingolipids in rat liver cells.


The incorporation of (14C) palmitate into glycerides and phospholipids of liver homogenates from fed and starved rats.


Metabolism of vitamin D3 in man.

Metabolism of vitamin D3 in man.


Isomers of glucosaminylphosphatidylglycerol.


The pyrophosphatase activity of pig kidney alkaline phosphatase and its inhibition by magnesium ions and excess of pyrophosphate. 1. Pig kidney enzyme resembles other non-specific alkaline phosphatases in its ability to hydrolyse inorganic pyrophosphate (PP(i)). 2. Studies of enzyme velocity as a function of PP(i) concentration show that Michaelis-Menten kinetics are obeyed when a constant PP(i)/Mg(2+) concentration ratio is maintained, but velocity-substrate concentration curves are sigmoid when the concentration of PP(i) is increased but that of Mg(2+) is kept constant. The enzyme is inhibited when the total PP(i) concentration is greater than the total concentration of Mg(2+). Pyrophosphatase activity is activated by Mg(2+), but if the concentration of the metal ion is increased to a value in excess of the total PP(i) concentration Mg(2+) is then strongly inhibitory. 4. It appears that the enzyme is most active towards the complex ion MgPP(i) (2-). The enzyme probably hydrolyses PP(i) (4-) also, but this is a poorer substrate and its competition with MgPP(i) (2-) leads to inhibition. At high Mg(2+) concentrations Mg(2)PP(i) is formed. This complex appears to be a potent inhibitor. 5. Sigmoid plots of v against s and of v against i result from interactions occurring between Mg(2+) and PP(i) (4-) leading to MgPP(i) (2-) and Mg(2)PP(i), and are not indicative of allosteric behaviour.


Continuous coupled assay for acetyl-coenzyme A production: correction of observed rates.


The metabolism of thymol by a Pseudomonas. 1. Pseudomonas putida when grown with thymol contained a meta-fission dioxygenase, which required ferrous ions and readily cleaved the benzene nucleus of catechols between adjacent carbon atoms bearing hydroxyl and isopropyl groups. 2. 3-Hydroxythymo-1,4-quinone was excreted towards the end of exponential growth and later was slowly metabolized. This compound was oxidized by partially purified extracts only when NADH was supplied; the substrate for the dioxygenase appeared to be 3-hydroxythymo-1,4-quinol, which was readily and non-enzymically oxidized to the quinone. 3. 2-Oxobutyrate (0.9 mole) was formed from 1 mole of 3-hydroxythymo-1,4-quinone with the consumption of 1 mole of oxygen; acetate, isobutyrate and 2-hydroxybutyrate (which arose from the enzymic reduction of 2-oxobutyrate) were also formed. 4. These products, which were produced only when the catechol substrate contained a third hydroxyl group, appeared to result from the enzymic hydrolysis of the ring-fission product.


Soluble and insoluble phosphofructokinase in rat muscle.


The effect of bilirubin on the human platelet.


Shigella keratitis: a case report.

Shigella keratitis: a case report.


Chronic granulomatous disease.

Chronic granulomatous disease.


Clinical staging of Wilms' tumor.

Clinical staging of Wilms' tumor.


Pagophagia and iron lack.

Pagophagia and iron lack.


Isolation of Japanese encephalitis virus from natural sources by means of plaque techniques in chick embryo monolayer cell cutlures.


Long persistence of anti-dengue antibodies in the serum of native residents of some Japanese main islands.


Assay of plasma angiotensin.

Assay of plasma angiotensin.


Prostaglandin stimulation of rat corticosteroidogenesis. Prostaglandins and their C20:(omega)6 fatty acid precursors are present in rat adrenal glands. Small doses of prostaglandins (PGE(1), PGE(2), or PGF(1infinity) 1.4 to 2.4 micromolar) increased steroidogenesis in the superfused adrenal glands obtained from hypophysectomized rats. This effect was mimicked in part by both adrenocorticotropin and its postulated intracellular intermediate adenosine 3', 5'-cyclic monophosphate; all three responses were inhibited by cycloheximide.


Involvement of suprahypothalamic structures in the hormonal feedback action of corticosteroids.


Angiotensinase activity in blood plasma of the rat.


The role of the liver in angiotensinogen production.


Comparison of right atrial and right ventricular single and paired pacing in the canine heart.


Mechanism of thiosulfate oxidation by Thiobacillus intermedius.


Fructose 1,6-diphosphatase of rat tissues and transplantable rat hepatomas.


A chick serum fraction that stimulates alkaline phosphatase activity in embryonic chick femora in culture.


Exogenous NADH oxidation and particulate fumarate reductase in Mycobacterium phlei.


Spin-labeling as a general method in studying antibody active site.


The relation between membrane structure and NADH: (acceptor) oxidoreductase activity of erythrocyte ghosts.


Terpene biosynthesis: formation of nerol, geraniol, and other prenols by an enzyme system from Pinus radiata seedlings.


Nonheme iron protein as a possible site of rotenone inhibition of mitochondrial NADH dehydrogenase.


Fructose diphosphatase from rabbit muscle. II. Amino acid composition and activation by sulfhydryl reagents.


Immunological studies of liver, kidney, and muscle fructose 1,6-diphosphatases.


Effects of D2O on the allosteric properties of bovine liver glutamic dehydrogenase.


Calcification in a mucus-producing carcinoma of the stomach. Case report.


The carinogenicity of N-hydroxy-N-2-fluorenyl-acetamide and the matabolism of N-2-fluorenyl-acetamide in Praomis (mastomys) natalensis.


Rates of incorporation of tritium-labeled thymidine into the mitochondrial and nuclear DNA of normal rat liver, regenerating liver, and four hepatomas with different growth rates and their host livers.


Distribution of a basic azo-dye-binding protein in normal rat tissues anc carcinogen-induced hepatomata.


The activity of neurons in the lateral geniculate body during wakefulness and natural sleep.


Signs of cerebral hypoxia in hyperventilation.


Propagation of mouse hepatitis virus (MHV-3) in monolayer cell cultures from liver of newborn mice.


Combined action of Rous sarcoma virus and chemical carcinogen in rats.


Hydrolysis of aminoacetonitrile: peptide formation.


Chemical structure of circulin B.

Chemical structure of circulin B.


D-5-Chloro-2-(alpha-hydroxybenzyl) benzimidazole and 1-alkyl-5-chloro-2-(alpha-hydroxybenzyl) benzimidazoles as inhibitors of poliovirus multiplication.


Positive correlation of responsiveness to catecholamines of the rat liver glycogenolytic receptor with other alpha-receptor responses.


Report of the panel for the symposium on immunity to selected equine infectious diseases.


Partial resection of the uterus for chorioadenoma destruens.


Role of alkyl substitution in 2,3-disubstituted and 3-substituted 4-quinazolones on the inhibition of pyruvic acid oxidation.


The synthesis and biological properties of some dibenzazepines and dibenzazonines related to protostephanine.


Stimulation of the reticuloendothelial system in tumor-bearing rats.


Isolation and biochemical characteristics of the nuclear envelope.


In vitro evidence of neutral collagenase activity in an invasive mammalian tumour.


Relation between collagen synthesis and collagen proline hydroxylase activity in mammalian cells.


Inhibition of rhinovirus by isatin thiosemicarbazone analogues.


Dysfibrinogenemia in a patient with primary hepatoma. First observation of an acquired abnormality of fibrin monomer aggregation.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 8-1969.


Crohn's disease: natural history and treatment.


The medical treatment of ulcerative colitis.


Nephropathy and viroses.

Nephropathy and viroses.


Care of the foaling mare and foal.

Care of the foaling mare and foal.


Quantitative histochemistry of hypothalamus. II. Thiamine pyrophosphatase, nucleoside diphosphatase and acid phosphatase in the activated supraoptic nucleus of the rat.


Primary malignancy of the liver in childhood.


Cytofluorometric study of mast cell polyanions. II. Adult rat peritoneal mast cells regenerating after polymyxin treatment.


Infectious mononucleosis--a current concept and treatment.


Rate of ethanol metabolism in fed and starved rats after thyroxine treatment.


Collagenolytic activity in middle ear cholesteatoma.


Isolation of virus from herpetic keratitis. Influence of idoxuridine on isolation rates.


Theories and known observations about hormonal treatment of endometriosis-in-situ, and endometriosis at the enzyme level.


Activation of cardiac adenyl cyclase by thyroid hormone.


Cloning of Burkitt lymphoma cells cultured in vitro.


Adenosquamous carcinoma of the nasal, oral and laryngeal cavities. A clinicopathologic survey of ten cases.


Primary Krukenberg tumor of ovary. Review of literature and case report.


Ultrastructure of a hemangiopericytoma and a glomus tumor.


Asymptomatic production of ACTH. Radio immunoassay in squamous cell, oat cell and adenocarcinoma of the lung.


Some epidemiologic aspects of neoplastic diseases in Israeli immigrant population. 3. Brain tumors.


Epidemiologic aspects of neoplastic diseases in Israeli immigrant population. II. Malignant neoplasms in childhood.


Rhabdomyoma of larynx. Ultrastructural study and comparison with granular cell tumors(myoblastomas).


Hepatoblastoma in the adult.

Hepatoblastoma in the adult.


Glomus tumors of the stomach.

Glomus tumors of the stomach.


Elastofibroma--a degenerative pseudotumor.


The picodna viruses. H, RV, and AAV.

The picodna viruses. H, RV, and AAV.


The use of cytotoxic drugs in the surgery of malignant disease.


The induction of cancer by combinations of viruses and other agents.


The evaluation of left ventricular myocardial function in patients undergoing cardiac surgery.


Cytomegalovirus mononucleosis following renal haemodialysis.


Medial and anterior tarsal tunnel syndrome.


The complications of Phisohex.

The complications of Phisohex.


Malignant mesenchymal tumours of the corpus uteri.


Adenoid cystic carcinoma of the palate.

Adenoid cystic carcinoma of the palate.


Reflexes and their relationship to behavioural state in the newborn.


Topical treatment of ulcerative mammary carcinoma by thiotepa compresses.


K+ minus dependent phosphatase activity observed in the presence of both adenosine triphosphate and Na+.


Citrate synthases: allosteric regulation and molecular size.


Simplification of mouse lactate dehydrogenase electrophoretic patterns.


The properties and subcellular distribution of phosphatidylinositol kinase in mammalian tissues.


Chloride ion as a modifier of 2',3'-cyclic phosphodiesterase purified from halophilic Vibrio alginolyticus.


The binding of ADP to myosin.

The binding of ADP to myosin.


On the nature of the free radical formed during the primary process of bacterial photosynthesis.


Cytochrome oxidase and its derivatives. IX. Spectrophotometric studies on the rapid reaction of ferrous cytochrome c oxidase with molecular oxygen under conditions of complete and partial oxygenation.


Prostaglandins.

Prostaglandins.


Fluoride toxicity in the mouse.

Fluoride toxicity in the mouse.


Immunofluorescence and cellular fraction studies on the multiplication of infectious canine hepatitis virus.


Effect of sodium desoxycholate on infectious canine hepatitis virus, and an additional description of a small associated virus.


Wilms' tumor metastiatic to mandible and oral mucosa. Report of a case.


Virus infections of the upper respiratory tract (abridged).


Virus infections of the upper respiratory tract.


Syndactyly and ring constrictions.

Syndactyly and ring constrictions.


Biogenic amines and the states of sleep.


Pleuro-pulmonary amoebiasis.

Pleuro-pulmonary amoebiasis.


Skeletal maturation of Danish school children in relation to height, sexual development, and social conditions.


Actinomycin D and transverse lines of growing bones.


The influence of hormone treatment on the natural evolution of the idiopathic nephrotic syndrome in childhood.


Corticotrophin secreetion capacity after long-term corticotrophin treatment in multiple sclerosis.


The blink reflex as a diagnostic procedure.


Pertussis vaccine research in the Rijks Instituut voor de Voklsgezondheid (the Netherlands).


Antibodies to the group II lumpy skin disease viruses in the sera of cattle in Kenya.


Anti-SV-40 complement-fixing antibodies in the serum of children.


Histochemical studies of the cestode Diphyllobothrium dendriticum Nitzsch, 1824.


Technology and learning in nursing.

Technology and learning in nursing.


Can nurses in general hospital participate in research.


Toward rehabilitating the geriatric patient.


Dynamics of change.

Dynamics of change.


Regional Medical Programs.

Regional Medical Programs.


International trends.

International trends.


Intervening when the patient is delusional.


Histological observations of cysticercus cyst walls of the liver of rats in relation to the precancerous stage in the Watanabe I tumor.


Assessment of skeletal age in the first five years of life.


The nursing role in a children's day hospital.


Antabuse program for alcoholics Racine County.


Manipulation by corticotropin and cortisol of aldosterone secretion in primary aldosteronism.


Suppressed plasma renin activity in hypertension.


Speaking out. On: the psychiatric aide, salaries and walk-out.


"Growth" and development.

"Growth" and development.


Combination chemotherapy in accelerated phase of chronic granulocytic leukemia.


Psychiatric drugs & trends. Guinea pigs behind bars.


Use of punishment procedures in management of behavioral difficulties of the severely retarded.


Endoplasmic reticulum and phenotypic variability in normal and neoplastic liver.


Histochemical studies on carbohydrate metabolism in the olfactory bulb of the rabbit.


Dimensional changes of a cristobalite investment by heating, cooling and reheating.


Peripheral compression lesions of the ulnar nerve.


Spontaneous paralysis of the anterior interosseous nerve.


The arcade of Frohse and its relationship to posterior interosseous nerve paralysis.


A search for the causes of severity in smallpox.


Surgical treatment of diseases of salivary glands.


Granular cell myoblastoma occurring in the upper lip: report of case.


Acute respiratory infections in aircrew under training with special reference to influenza and adenovirus infections.


Effects of nervous system lesions on phantom experience in amputees.


Isopycnic separation of subcellular components from poliovirus-infected and normal HeLa cells. A rapid method for determining the density of ribonucleoprotein particles and complexes has been developed. The method involves glutaraldehyde fixation of sucrose gradient fractions and immediate centrifugation for 5 hours through preformed cesium chloride gradients. There is little or no aggregation of particles, so that components which co-sediment in sucrose gradients are resolved by the cesium chloride gradient. By this method the densities of HeLa cell ribosomes, polyribosomes, and subribosomal particles have been determined. Furthermore, the poliovirus replication complex has been separated from polyribosomes and its density has been found to be unaffected by treatment with ethylene-diaminetetraacetic acid.


Histone phosphorylation: stimulation by adenosine 3',5'-monophosphate. Adenosine cyclic 3',5'-monophosphate at a concentration of 10-(7)M causes a four-to sixfold increase in the rate of histone phosphorylation catalyzed by a liver enzyme preparation. This observation suggests a mechanism for the induction of RNA synthesis by those hormones that cause increases in the concentration of cyclic AMP.


Colistin sulfate versus sodium colistimethate.


Vasopressin test in an adrenocorticotrophic-hormone-producing tumor.


Cranial mononeuropathy as the first sign of intracranial metastases.


Congenital adrenal hyperplasia secondary to 17-hydroxylase deficiency. Two sisters with amenorrhea, hypokalemia, hypertension, and cystic ovaries.


Experimental dietary hepatoma in rainbow trout. The role of cottonseed meals and other diets.


Reflex regulation of arterial pressure during sleep in man. A quantitative method of assessing baroreflex sensitivity.


Fine structural localization of acetylcholinesterase at a cholinergic vasodilator nerve-arterial smooth muscle synapse.


Tissue response to intraperitoneal asbestos with preliminary report of acute toxicity of heart-treated asbestos in mice.


Kinetics of NAD reduction in the nucleus and the cytoplasm.


Alk-1-enyl ethers of glycerol and thanediol.


On the localization of poly ADPribose synthetase in the nucleus.


A serologic study on some respiratory viral and rickettsial infections in a rural community.


Effects on ACTH release, of intrapituitary injections of posterior pituitary hormones and several amines in the hypothalamus.


Role of catecholamine and adenosine in the ischemic response following release of a renal artery occlusion.


A simplifiel method for the preparation of high titer polyoma virus.


Electron microscopic study of virus particles in experimentally mixed tumor cells.


Various antibody holding status against viruses in Cercopithecus aethiops and Macaca irus. I. The presence of neutralizing antibodies to measles virus and polio virus.


Various antibody holding status against viruses in Cercopithecus aethiops and Macaca irus. II. The presence of neutralizing antibody against herpes virus, ECHO virus and simian virus 40.


The use of beta-propiolactone and ultraviolet irradiation for the treatment of viral contamination of canine serum.


Neurological diseases in Korea.

Neurological diseases in Korea.


Absorption studies in neurological disorders.


Peripheral neuropathy and carcinoma.

Peripheral neuropathy and carcinoma.


Astereognosis not of cortical origin.

Astereognosis not of cortical origin.


A malignancy involving the cranial nerves with a possible geographic distribution.


Pituitary vascularity and ACTH release in rats with hypothalamic lesions.


The thoracic glanglia of the grasshopper, Melanoplus differentialis: fine struc- ture of the perineurium and neuroglia with special reference to the intracellular distribution of phosphatases.


Influence of some streptomyces antibiotics on the inner ear of the guinea pig. Electrophysiological and histological study.


The deposition and metabolism of polyphosphoinositides in rat and guinea-pig brain during development. 1. The deposition of triphosphoinositide and diphosphoinositide in rat and guinea-pig cerebral hemispheres during growth was measured. 2. The maximum increase in concentration of both of these phospholipids occurs during the period of myelination, but in the rat some di- and tri-phosphoinositide is present before significant myelination begins. 3. In guinea-pig cerebral hemispheres the polyphosphoinositides remaining after post-mortem breakdown are selectively enriched in dissected white matter compared with grey matter. 4. The polyphosphoinositides in the cerebral hemispheres of rats were labelled with injected (32)P very rapidly; the specific radioactivities were in the order triphosphoinositide>diphosphoinositide>monophosphoinositide>total lipid phosphorus. 5. The synthesis of triphosphoinositide in rat forebrain occurs at an appreciable rate before, and at the start of, myelination, but the amount formed per gram of tissue is four to five times greater in adult rat brains, thus maintaining a constant turnover time (about 1hr.) for the whole triphosphoinositide fraction. This indicates that the rapid turnover of triphosphoinositide is independent of myelin deposition. 6. The specific radioactivity of the brain acid-soluble phosphorus pool referred to a constant dose of (32)P/g. body wt. falls rapidly with age, reaching a minimum at 13-14 days, and then rises again. The specific radioactivities of the polyphosphoinositides reflect this change. 7. Part of the polyphosphoinositides in rat and guinea-pig cerebral hemispheres is rapidly hydrolysed post mortem leaving a stable portion resistant to further breakdown. 8. The rate and extent of post-mortem hydrolysis of the polyphosphoinositides in both species decrease with age. 9. After (32)P labelling, the specific radioactivity of the triphosphoinositide remaining in the cerebral hemispheres of the rat after post-mortem breakdown is lower than the original triphosphoinositide fraction, suggesting two metabolically distinct pools.


The metabolism of polyphosphoinositides in hen brain and sciatic nerve. 1. The distribution of individual phospholipids was determined in hen brain and compared with that in sciatic nerve obtained in a previous investigation. Sciatic nerve is more enriched in the myelinic phospholipids ethanolamine plasmalogen, phosphatidylserine and sphingomyelin, but it contains relatively less triphosphoinositide, and much less diphosphoinositide, than the brain. 2. The course of incorporation of intraperitoneally injected (32)P into the acid-soluble phosphorus, phosphoinositides and total phospholipids of hen brain and sciatic nerve was followed. Although the maximum specific radioactivity in sciatic nerve of acid-soluble phosphorus is 4.5 times, and that of triphosphoinositide six times, that in the brain, the relative rate of triphosphoinositide phosphorus synthesis per gram of brain is three times that in sciatic nerve. 3. Administration of the demyelinating agent tri-o-cresyl phosphate to hens has no significant effect on the amounts or the rate of (32)P incorporation into the total phospholipids of the sciatic nerve. However, the rate of incorporation of (32)P into triphosphoinositide, although not its concentration, is raised from the first day after administration of the drug and remains thus 13 and 23 days later. 4. The incorporation of (32)P into polyphosphoinositides of hen brain slices in vitro was studied. The recovery of triphosphoinositide from the slices is markedly increased in the presence of EDTA, although the rate of incorporation of (32)P is unaffected. The incorporation of (32)P is dependent on the presence of Mg(2+) and Ca(2+) in the medium, and is decreased when Na(+) is replaced with K(+) or cholinium ions.


Gluconeogenesis in developing rat kidney cortex. 1. Gluconeogenesis in developing rat kidney cortex was studied by assaying the activities of two enzymes, glucose 6-phosphatase and phosphoenolpyruvate carboxykinase, and by measuring glucose formation in tissue slices. 2. Glucose 6-phosphatase and phosphoenolpyruvate carboxykinase are present in late foetal (21-22-day-old) tissue and increase rapidly postnatally. Maximum activity of phosphoenolpyruvate carboxykinase occurs at 7 days of age, followed by a decline to the adult level. Glucose 6-phosphatase activity rises during the first 2 postnatal weeks and then declines. 3. Late foetuses synthesize glucose from both pyruvate and l-glutamate. The rate increases during the first 2 weeks to above adult levels. Synthesis is always higher from pyruvate than from glutamate. 4. The effect of 24hr. starvation was studied in perinatal animals. The results indicate that the ability to increase the rate of glucose synthesis as a result of starvation is not present at birth, but develops some time after the second postnatal day.


The role of imidazol-5-yl-lactate-nicotinamide-adenine dinucleotide phosphate oxidoreductase and histidine-2-oxoglutarate aminotransferase in the degradation of imidazol-5-yl-lactate by Pseudomonas acidovorans.


Characteristic and selective aggregate-forming activity of neoplastic cells in culture.


Lactate dehydrogenase isoenzymes of sperm cells and tests. 1. The kinetic and metabolic properties of lactate dehydrogenase isoenzyme LDH(x) from human sperm cells and rat testes were studied. 2. LDH(x) shows a sensitivity to inhibition by stilboestrol diphosphate, urea and guanidinium chloride different from that of the LDH-H(4) and LDH-M(4) isoenzymes. 3. About 10 and 20% of the total lactate dehydrogenase activity of testes and sperm cells respectively were associated with particulate fractions. In sperm cells 11% was localized in the middle piece and 18.8% in the head fraction. LDH(x) was found in all particulate fractions of sperm cells. The middle piece contained 41.0% of total LDH(x) activity and showed high succinate dehydrogenase activity. 5. The pH-dependence of lactate/pyruvate and NAD(+)/NADH concentration ratios were estimated. Lactate dehydrogenase in sperm cells has maximal activity with NADH as coenzyme at pH7.5 and with NADPH as coenzyme at pH6.0. At pH6.0 a 10% greater oxidation of NADPH than of NADH was found. At acid pH lactate hydrogenase may function as an enzyme bringing about transhydrogenation from NADPH to NAD(+). 6. In agreement with the stoicheiometry of the lactate de- hydrogenase reaction, the lactate/pyruvate concentration ratio decreased with increasing pH. 7. The lactate/pyruvate and NAD(+)/NADH concentration ratios were estimated with glucose, fructose and sorbitol as substrates and as a function of time after addition of these substrates. During a 20min. period after the addition of the substrates, changes in lactate/pyruvate and NAD(+)/NADH concentration ratios were noticed. Increasing concentration of the substrates mentioned gave rise to asymptotic increases in lactate and pyruvate. 8. Sorbitol did not act as a substrate for LDH(x). 9. The findings described are consistent with the idea that LDH(x) is different from other known lactate dehydrogenase isoenzymes, but that it has a metabolic function similar to that of the isoenzymes of other tissues.


A review of neuroendocrinology, 1966-67.


Electron spin resonance study on the interaction between the chemical carcinogens and tissue components. 3. Determination of the structure of the free radical produced either by stirring 3,4-benzopyrene with albumin or incubating it wtih liver homogenates.


Effect of growth of rhodamine sarcoma in rat on some liver enzyme activities.


Cytological individuality of hepatoma cell lines derived from a single Donryu rat.


The distribution of thiamine diphosphate-phosphohydrolase in the neurosecretory nuclei of the rat following osmotic stress.


Biochemical basis of tissue acidosis.

Biochemical basis of tissue acidosis.


"Intracerebral steal" in man recorded by a heat clearance technique.


Urinary androsterone, etiocholanolone, and dehydroepiandrosterone responses to 72-hr. avoidance sessions in the monkey.


"Over-all" hormonal balance as a key to endocrine organization.


A review of psychoendocrine research on the pituitary-adrenal cortical system.


Booster effect of oral poliovaccine. Trials in persons previously immunized with inactivated vaccine.


Enzymatic conversion of phenylpyruvate to phenylacetate.


Aminopropanol dehydrogenase activity in rat-liver preparations.


The enthalpy change in the hydrolysis of the phosphate esters of myo-inositol.


Metabolism of 2,3-diphosphoglycerate and glycolysis in humna red blood cells under the infleucne of dipyridamole and inorganic sulfur compounds.


Perodate oxidation, pKa and conformation of hexuronic acids in polyuronides and mucopolysaccharides.


ACTH release by purified hypothalamic CRF preparation as assayed by intrapituitary microinjection.


Rubredoxin from a nitrogen-fixing variety of Desulfovibrio desulfuricans.


3 alpha-hydroxysteroid: NAD oxidoreductase activity in crystalline preparations of 20 beta-hydroxysteroid: NAD oxidoreductase.


Genetics of octanol dehydrogenase in Drosophila metzii.


Glycosyl glycerides from Streptococcus hemolyticus strain D-58.


Effects of steroid hormones on drug-metabolizing enzyme systems in liver microsomes.


The two deoxyribonucleases, K1 and K2, isolated from mycelia of Aspergillus oryzae. II. Determination of 5'- and 3'-termini of the DNA fragments produced by DNases K1 and K2.


On the deoxyribonucleases, K1 and K2, isolated from mycelia of Aspergillus oryzae. I. Isolation and purification of DNases K1 and K2.


Quantitative determination of Clostridium welchii phospholipase C activity in aqueous reaction medium.


Application of the isotope dilution method to microanalytical determination of five classes of sphingoglycolipids in tissues. I. Preparation and properties of tritium labelled sphingoglycolipids.


Use of field modulation with boxcar integrator to measure relaxation time in electron spin resonance experiments.


Interaction between normal and transformed bovine fibroblasts in culture. II. Cells transformed by polyoma virus.


Inhibition of interferon production by chloroquine diphosphate.


On some problems of complications in the central nervous system due to vaccination.


Differential effects of hypothalamic deafferentation on responses to different stresses.


Interference phenomenon in the culture of chick embryo cells infected with Schmidt-Ruppin strain of Rous sarcoma virus.


Cytomegalovirus infection in children in Japan.


Enhanced replication of Newcastle disease virus in cell culture co-infected with certain other viruses.


Disseminated intravascular coagulation in the newborn.


Comparison of the pressor activity and the renin content of the renal venous effluent in patients suspected of suffering from surgically remediable hypertension.


Further studies on renal lesions of New Zealand black (NZB-BL) strain mice.


Responses to steroids of Xiphophorus ACTH cells in organ culture.


Non-specific esterase in cerebral tumors. A histochemical study.


Visual form agnosia. A specific defect in visual discrimination.


Trichloroethylene neuropathy.

Trichloroethylene neuropathy.


Concentration by Diaflo ultrafiltration of murine leukemia and sarcoma viruses grown in tissue cultures.


Studies of the significance of tumor antigens in induction and repression of neoplastic diseases: presidential address.


Electron microscopy of the irises of chickens with spontaneous ocular leukosis.


Morphologic and biologic characteristics of the canine cutaneous histiocytoma.


Enzymes of thymidine triphosphate synthesis in selected Morris hepatomas.


Acceleration of liver cell proliferation in vitro by N-nitrosodimethylamine.


Central cholinergic pathways.

Central cholinergic pathways.


Studies on phenolic  steroids in human subjects. 8. Metabolism of estriol-16 alpha-glucosiduronate. 6,7-(3)H-Estriol-16alpha-glucosiduronate-(14)C was administered to eight women (nine studies) by several routes: both injection and infusion (300 min) into the cubital vein, injection into the portal vein system, ingestion and instillation into the duodenum, jejunum, and ileum. Urine, collected from 0-2, 2-4, 4-8, 8-12, and 12-24 hr, was analyzed by countercurrent distribution for its content of radioactive 3- and 16-glucosiduronate (E(3)-3Gl,E(3)-16Gl) and sulfoglucosiduronate (E(3)-3S,16Gl) of estriol as well as for (3)H/(14)C ratio of each conjugate. After peripheral injection 50-60% of the injected E(3)-16Gl was excreted unchanged along with about 5% as E(3)-3S,16Gl with an unchanged (3)H/(14)C ratio, indicating direct sulfation of the injected E(3)-16Gl. During a 300 min infusion, urinary excretion closely resembled that following injection. But 2-4 hr after the end of the infusion excretion of E(3)-3S, 16Gl stopped, excretion of E(3)-3Gl (17%/24 hr) with an elevated (3)H/(14)C ratio started, and excretion of E(3)-16Gl continued (70%/24 hr), but with a rapidly increasing (3)H/(14)C ratio. This indicated sequestration in a sluggishly metabolizing compartment where two processes occurred: (a) extensive hydrolysis of E(3)-16Gl followed by reconjugation at either C3 or C16 with unlabeled uridine diphosphate glucuronic acid (UDPGA), thereby increasing the (3)H/(14)C ratio; and (b) transconjugation from C16 to C3, thereby producing E(3)-3Gl with finite (3)H/(14)C ratios. Instillation into various segments of the small intestine produced results qualitatively similar to those after intravenous infusion, whereas ingestion and intraportal injection resembled peripheral intravenous injection. Therefore, we have postulated the possibility of an enteric circulation (in addition to an enterohepatic circulation) in which the steroid or its conjugates are transported into the small intestine in the succus entericus, modified, and then reabsorbed and excreted in the urine-a process which requires several hours.


Renal metabolic response to acid base changes. I. Enzymatic control of ammoniagenesis in the rat. Experiments were done on rats to investigate the nature of the renal response to metabolic acidosis and the changes in enzyme activity associated with increased ammoniagenesis. When metabolic acidosis was induced with oral feeding of ammonium chloride for 48 hr, there was an increase of activity of the enzyme phosphoenolpyruvate carboxykinase (PEPCK) in whole kidneys as well as in the kidney cortex. There was no change in PEPCK in liver, and glucose-6-phosphatase showed no change in kidney or liver in response to metabolic acidosis. The increase in PEPCK activity in kidney cortex varied with the degree of acidosis and there was a close correlation between cortical PEPCK activity and urinary ammonia. Kidney cortex mitochondrial PEPCK did not change in response to metabolic acidosis. An increase in PEPCK occurred as early as 6 hr after NH(4)Cl feeding, before there was any increase in kidney glutaminase I activity. Rats fed sodium phosphate, or given triamcinolone intramuscularly, developed a metabolic alkalosis, but there was increased urinary ammonia and an increase in activity of renal cortical PEPCK. Triamcinolone plus ammonium chloride induced a greater increase of PEPCK activity than triamcinolone by itself; on the contrary, the rise of glucose-6-phosphatase induced by triamcinolone was not enhanced by acidosis. Glucose-6-phosphatase from control and acidotic rats had identical kinetic characteristics. The results indicate that increased PEPCK activity is constantly related to increases of urinary ammonia. It is proposed that the increase of PEPCK activity is the key event in the ammoniagenesis and gluconeogenesis which follow on metabolic acidosis.


Central cholinergic transmission and its behavioral aspects. Historical introduction.


Central cholinergic transmission and its behavior aspects. Introductory remarks.


Application of control systems theory to physiology. Adrenal glucocorticoid control system.


Studies on phenolic steroids in human subjects. IX. Role of the intestine in the conjugation of estriol. In order to compare the enteric circulation of estriol-16alpha-glucosiduronate (see preceding paper) with that of estriol (E(3)), labeled estriol was administered to six women by several routes: both injection and infusion (300 min) into the cubital vein, injection into the portal vein system, ingestion and instillation into the jejunum and ileum. Urine, collected from 0-2, 2-4, 4-8, 8-12, and 12-24 hr, was analyzed by countercurrent distribution for its content of radioactive 3- and 16-glucosiduronate (E(3)-3Gl,E(3)-16Gl) and sulfoglucosiduronate (E(3)-3S,16Gl) of estriol. After peripheral injection of E(3), E(3)-16Gl was excreted rapidly and E(3)-3S,16Gl at a slower and more constant rate. E(3)-3Gl was barely detectable after infusion. After injection of E(3) into the portal vein, the excretion of E(3)-3S,16Gl was greater and quicker than after peripheral injection. Even in a subject with a complete bile fistula, the urinary excretion of E(3)-3S,16Gl was essentially unchanged. Ingestion also produced the same result. Only after instillation into the ileum was a large and rapid excretion of E(3)-3Gl obtained, whereas the excretion of E(3)-3S,16Gl, and E(3)-16Gl were depressed. These results together with those of the preceding paper suggest that E(3) does not readily appear in the small intestine except via a hepatoenteric circulation that produces very little E(3)-3Gl. When present in the distal segment of the small intestine, however, absorption, conjugation, and elimination proceed readily.


Is the central cholinergic nervous system overexploited?


Invited discussion: postreinforcement synchronization and the cholinergic system.


Behavioral aspects of cholinergic transmission.


Ventricular function in noncardiacs with alcoholic fatty liver: role of ethanol in the production of cardiomyopathy. Since many patients with cardiomyopathy have a history of chronic ethanolism often associated with malnutrition, we have evaluated left ventricular (LV) function in alcoholics with fatty liver, who had no clinical evidence of cardiac or nutritional disease. During an afterload test of LV function the pressor response to angiotensin evoked a threefold rise of enddiastolic pressure in the alcoholic group which was substantially greater than the 4 mm Hg rise in control subjects. The stroke volume and stroke work response in the noncardiac alcoholic was significantly less than in controls. Diminished LV function was corroborated in the noncardiac alcoholic at rest, using a contractility index. To evaluate the dose-response relationship of ethanol in the production of cardiac malfunction, two groups of noncardiac alcoholic subjects were studied acutely at low and moderate dose levels. After 6 oz, ventricular function, myocardial blood flow, and metabolism were not significantly affected. After 12 oz, there was a progressive rise of end-diastolic pressure and decrease of stroke output at a mean blood alcohol level of 150 mg/100 ml, reverting toward control by 4 hr. The coronary effluent transiently evidenced leakage of cell constituents, despite an increase of coronary blood flow, suggesting a direct but reversible cardiac injury. Myocardial extraction of triglyceride was enhanced, whereas FFA uptake was reduced. A possible role of myocardial triglyceride accumulation in heart muscle was considered in pathogenesis. Chronic ingestion of 16 oz of Scotch daily by an alcoholic subject while on a normal diet produced, after 12 wk, a progressive increase of heart rate and size, circulation time, and venous pressure, and a ventricular diastolic gallop. Normal values were restored within 7 wk after interrupting alcohol. These several studies suggest that the cumulative effects of repeated ingestion of ethanol in intoxicating doses can produce diminished LV function before clinical evidence of cardiac abnormality, or heart disease not necessarily related to malnutrition.


Studies of the zeta potential of cells and a silica particle in varying concentrations of albumin, calcium, sodium, plasma, and bile.


Glomus tumor simulating nerve root compression and Raynaud's phenomenon. Case report.


Solitary intramedullary carcinomatous metastasis in the spinal cord. Case report.


Cytochemistry of sea urchin gametes. 3. Acid and alkaline phosphatase activity of spermatozoa and fertilization.


Inhibition of angiotensinases in the rat plasma and kidney.


A new cell line of calf kidney and its susceptibility to viruses.


Studies on parainfluenza virus infections among infants and children in Sendai. I. Isolation and identification methods of parainfluenza viruses.


Significance of acetylcholinesterase in central synaptic transmission.


Interference with virus infection induced by RNA in chick embryo cells.


Effect of actinomycin-D on DNA synthesis in African green monkey kidney cultures uninfected and infected with SV40.


Studies on parainfluenza virus infections among infants and children in Sendai. II. Serologic and epidemiologic investigation.


Effect of corticotropin and adrenocortical hormone on the blood flow through brown adipose tissue in the rat.


Sleep cycle characteristics in infants.

Sleep cycle characteristics in infants.


Proton magnetic resonance studies of human cyanomethemoglobin.


The transcription of the SV40 genome in productively infected and transformed cells.


Human cell strains susceptible to focus formation by human adenovirus type 12.


The role of the lac promotor locus in the regulation of beta-galactosidase synthesis by cyclic 3',5'-adenosine monophosphate.


Inactivation and mutagenesis due to the photodynamic action of acridines and related dyes on extracellular bacteriophage T4B.


Interaction of aminoacyl transferase II and guanosine triphosphate: inhibition by diphtheria toxin and nicotinamide adenine dinucleotide.


The mechanism of the pyruvate, phosphate dikinase reaction.


Conformational properties of polyformycin: a polyribonucleotide with individual residues in the syn conformation.


Biological activity of 25-hydroxycholecalciferol, a metabolite of vitamin D3.


Morphological and histochemical properties of the regenerated mucosa in the ileum of the rat.


Drug induced inhibition of fibrin stabilization.


Basic science and pancreatic surgery. A study in anatomy, physiology and pathology.


Pitfalls in the diagnosis of pneumonia.

Pitfalls in the diagnosis of pneumonia.


Pneumonia in childhood.

Pneumonia in childhood.


In vitro products of a membrane-free foot-and-mouth disease virus ribonucleic acid polymerase.


The effect of actinomycin D on the transcription and replication of simian virus 40 deoxyribonucleic acid.


Viropexis of vesicular stomatitis virus by L cells.


Kinetics of neutralization of sensitized herpes simplex virus with antibody fragments.


Metastasizing nasopharyngeal angiofibroma. A case report.


Maxillary sinus angiofibroma treated with cryosurgery.


Conjoint family therapy for alcoholics.

Conjoint family therapy for alcoholics.


Effects of DNA on free radical production from the carcinogens 4-nitro-and 4-hydroxyamino-quinoline 1-oxides in aqueous medium at 77 degrees K.


Influence of particle size on physicochemical properties of pharmaceutical powders. VII. Fluidity and packing property of binary mixtures.


Kalafungin, a new broad spectrum antibiotic. Isolation and characterization.


Mycophenolic acid: antiviral and antitumor properties.


Studies on the EPR and light absorption spectra of horse erythrocyte catalase and its derivatives.


Purification and properties of ascorbate oxidase from cucumber.


Oxidation and reduction of copper proteins: note added to the previous report on the state and activity of copper atoms in copper proteins.


The purification of prenyltransferase and isopentenyl pyrophosphate isomerase of pumpkin fruit and their some properties.


Antiviral activity of selected Catharanthus alkaloids.


A glucose-responsive insulinoma.

A glucose-responsive insulinoma.


Acute lymphocytic leukemia in children: maintenance therapy with methotrexat administered intermittently. Acute Leukemia Group B.


A case of Klippel-Weber syndrome.

A case of Klippel-Weber syndrome.


Experimental bronchopneumopathy due to SO2 in the dog.


Histochemical demonstration of glycogen synthetase in the normal human epidermis.


Potentiation by smooth muscle stimulants of the hypogastric nerve--vas deferens preparation from normal and castrated guinea-pigs.


Fatty acid composition of cerebrosides and cerebroside sulphatides in cerebral oedema.


Abnormal estrogen responses to ACTH  stimulation in the polycystic ovary syndrome.


Studies on antigens from axenically cultivated Entamoeba histolytica and Entamoeba histolytica-like amebae.


Studies on parainfluenza type 2 and 4 viruses obtained from patients with common colds. Four agents which were obtained from adults with common colds were cultivated and identified-one was influenza type B and the others were parainfluenza viruses of types 2 and 4. Their cultivation was assisted by the use of organ cultures of human embryo tracheal or nasal epithelium. They infected and caused typical common colds in volunteers.


Studies on lipoic acid uptake by bacteria. 3. Intracellular distribution of enzymes.


The in vitro product of poliovirus polymerase.


Inhibition by adrenocorticotrophin of gonadotrophin-induced ovulation in immature female rats.


Adenosine 3',5'-monophosphate (cyclic AMP) as the mediator of the actions of melanocyte stimulating hormone (MSH) and norepinephrine on the frog skin.


Effect of chlorpropamide on the permeability of the urinary bladder of the toad and the response to vasopressin, adenosine-3',5'-monophosphate and theophylline.


Morphology and hormone production of rat hypophyseal and adrenocortical tissue in tissue culture.


Growth hormone and phosphorylase activity in adipose tissue.


Effect of high sodium intake on the response of the rat adrenal to angiotensin II.


Inhibitory effect of immune serum on carcinogenesis in mice neonatally infected with murine sarcoma virus (Harvey).


Lingually induced inhibition of masseteric motoneurones.


Interaction of Rous sarcoma virus with rat embryo fibroblasts of inbred Lewis strain in vitro.


Labelled tocopherols in man. Intestinal absorption and thoracic-duct lymph transport of dl-alpha-tocopheryl-3,4-14C2 acetate dl-alpha-tocopheramine-3,4-14C2 dl-alpha-tocopherol-(5-methyl-3H) and N-(methyl-3H)-dl-gamma-tocopheramine.


Enzyme activity and immunoelectrophoretic pattern in the guinea pig in avitaminosis C.


Studies on adenylylstreptomycin, a product of streptomycin inactivation by E. coli carrying the R-factor.


The structure of the guanido amino acid moiety of viomycin.


Mechanism of action of phenomycin: comparative study with diphtheria toxin.


Electron microscopic studies on mode of action of polymyxin.


Fractionation and characterization of the plasma and mesosome membrane of Listeria monocytogenes. Protoplasts of Listeria monocytogenes strain 42 were fractionated after control lysis on a Ficoll (a polysucrose) density gradient. Visually, five zones could be recognized in the gradient. The first one was composed of amorphous cytoplasmic solutes (fraction 1a) and a mixture of particles (fraction 1b). These were: (i) light particles that were lipase-sensitive and composed of six subunits and (ii) heavy particles, sensitive to ribonuclease and devoid of fine structure. The second zone consisted of tubules and vesicles still harboring cytoplasmic components (fraction 2), whereas the third zone contained only empty vesicles and protoplast ghosts (fraction 3). The material congregating into the fourth zone was morphologically identical to that of the third (fraction 3a). The fifth and heaviest zone contained a mixture of (i) particles without any substructure and (ii) partly lysed protoplasts (fraction 4). Fractions 1b and 4 were the richest in nucleic acids (ribonucleic acid, 11.4 and 9.4%, respectively; deoxyribonucleic acid, 5.1 and 4.8%, respectively), whereas fraction 1b had the highest protein contents (74.6%). Phospholipids were mainly found in fractions 2 and 3. Except for fraction 1, all materials contained significant amounts of protein-bound phosphorus. The main concentrations of four enzymes were: glucose-6-phosphate dehydrogenase (fraction 1a); adenosine triphosphatase and reduced nicotinamide adenine diphosphate oxidase (fraction 3); nitro blue tetrazolium chloride reductase (fraction 2). Fractionation of strain 42 after addition of (32)P during the mid-log phase of growth revealed that the radio-activity was mainly detected in fraction 1b, when growth in the presence of the marker was allowed for 10 min, and in fraction 2, when growth was allowed for 90 min. The vesicles of fraction 2, often tubular, are probably of mesosomal origin, whereas those of fraction 3, which are always spherical, represent, most likely, the bulk of the cell plasma membrane. Our data showed slight chemical differences between these two fractions, but the differences in enzymatic activities and lipid-phosphorus incorporation during long pulse experiments were most dramatic.


Acquired cytomegalovirus infection and retardation of somatic growth.


The effect of theophylline on amine-induced cardiac phosphorylase activation and cardiac contractility.


Drinking caused by the intracranial injection of angiotensin into the rat.


Lung function studies after the intralymphatic injection of emulsions of ethiodol in dogs.


A practical method for the fractionated fluorimetric estimation of estrogens in pregnancy urine.


The influence of anti-inflammatory drugs on the glycoprotein biosynthesis in vitro.


The detection of androsta-4,16-dien-3-one in peripheral plasma of adult men.


The role of human fetal adrenals in steroidogenesis: effect of adrenocorticosterone on urinary excretion of estrogens in pregnancy.


Fever therapy in aestival conjunctivitis.


Isolated adrenocorticotrophic hormone deficiency in acute intermittent porphyria.


Studies on the biosynthesis of nicotinamide adenine dinucleotide (NAD) in mammals and its regulatory mechanism. II.


Effect of prostaglandin E-1 (PGE-1) on the vascular responsiveness to norepinephrine and angiotensin in the anesthetized rat.


An agar disc method for testing the sensitivity of microorganisms to antibiotics.


Extracellular unit recording from the thalamus in chronically implanted patients with movement disorders.


Hydrocephalus as a sequela of experimental myxovirus infections.


Changes of phenylalanyl-tRNA of Morris hepatoma 5123 revealed by reverse phase chromatography.


Studies with the radioimmunoassay for angiotensin II, and its application to measurement of renin activity.


Retrobulbar blockade in Endotoxin and horse-serum induced uveal response in rabbits.


Stimulation and suppression of aldosterone secretion in patients with an aldosterone-producing adenoma.


Plasma dehydroepiandrosterone sulfate in normal and pathological conditions.


Role of renin in adaptation to sea water in euryhaline fishes.


Effect of corticotrophin and cortisone on the incorporation of 3H-thymidine into the deoxyribonucleic acids (DNA) of the regenerating liver in the white rat.


Hypothalamo-pituitary-adrenal response during major surgical stress.


Pituitary-adrenal function in patients given long-term adrenal steroid therapy.


Congenital ocular anomalies in 800 mentally deficient patients.


Effect of corticotropin on gastric acid, pepsin, and mucus secretion in dogs with fistulas.


Ulceration of intramural gastric neoplasms.


Blood pressure changes during EEG-monitored sleep. A comparative study of hypertensive and normotensive Negro women.


Effect of an erotic movie on the sleep and dreams of young men.


Activation of adenyl cyclase by glucagon in cat and human heart.


Enzyme histochemical reactions of mouse ova.


Interaction of cocaine with angiotensin and tyramine on the isolated rabbit aortic strips.


Actions of interferon in tissue cultures harboring mouse leukemia virus.


Differentaiation in vitro of chick embryo adrenal glands.


Role of adenovirus in the causation of acute respiratory disease in Egypt.


Amino acid fortification and the protein problem.


Pseudomonas aeruginosa keratitis: mixed infections of the eye.


Photochemistry of antibiotics. I. Oxidative coupling of o-aminophenol by photoirradiation.


Glycolipids from rat kidney.

Glycolipids from rat kidney.


Studies on chemically modified cytochrome c. I. The acetylated cytochrome c.


Specificity of phenotypic adaptation of Bacillus cereus to tetracycline.


Chemically mediated transmission at a giant fiber synapse in the central nervous system of a vertebrate. The hatchetfish, Gasteropelecus, possesses large pectoral fin adductor muscles whose simultaneous contraction enables the fish to dart upwards at the approach of a predator. These muscles can be excited by either Mauthner fiber. In the medulla, each Mauthner fiber forms axo-axonic synapses on four "giant fibers," two on each side of the midline. Each pair of giant fibers innervates ipsilateral motoneurons controlling the pectoral fin adductor muscles. Mauthner fibers and giant fibers can be penetrated simultaneously by microelectrodes close to the synapses between them. Electrophysiological evidence indicates that transmission from Mauthner to giant fiber is chemically mediated. Under some conditions miniature postsynaptic potentials (PSP's) are observed, suggesting quantal release of transmitter. However, relatively high frequency stimulation reduces PSP amplitude below that of the miniature potentials, but causes no complete failures of PSP's. Thus quantum size is reduced or postsynaptic membrane is desensitized. Ramp currents in Mauthner fibers that rise too slowly to initiate spikes can evoke responses in giant fibers that appear to be asynchronous PSP's. Probably both spikes and ramp currents act on the same secretory mechanism. A single Mauthner fiber spike is followed by prolonged depression of transmission; also PSP amplitude is little affected by current pulses that markedly alter presynaptic spike height. These findings suggest that even a small spike releases most of an immediately available store of transmitter. If so, the probability of release by a single spike is high for any quantum of transmitter within this store.


A rectifying electrotonic synapse in the central nervous system of a vertebrate. The adductor muscles of the pectoral fins of the hatchetfish Gasteropelecus are innervated by bilateral pools of about 40 motoneurons which lie primarily in the first spinal segment. A pair of giant fibers on each side of the medulla send processes ventroposteriorly to the motoneuron pools. Electrophysiological evidence indicates that giant fibers are presynaptic to ipsilateral motoneurons, but not to contralateral ones. Transmission across the giant fiber, motoneuron synapse is electrically mediated as is indicated by direct measurement of electrotonic spread in either direction across the synapse, and by the extremely short latency of the giant fiber postsynaptic potentials (PSP's) in the motoneuron. The coupling resistance across the synapse was calculated from measurements of input and transfer resistance. The coupling resistance rectifies in such a way as to facilitate spread of depolarization from giant fiber to motoneuron, and to oppose transmission in the opposite direction. As a consequence of rectification, the giant fiber PSP in a motoneuron is augmented by hyperpolarization of the motoneuron. The coupling resistance calculated on the basis of this effect is in good agreement with calculations from input and transfer resistance data. Rectification at the electrotonic synapses may permit the motoneurons to act in small swimming movements as well as to fire synchronously in an extremely fast escape reflex mediated by Mauthner and giant fibers.


Distortions of normal bone cell metabolism induced in multiple myeloma.


Estradiol production after ovariectomy for carcinoma of the breast.


Goodbye to the menopausal ovary.

Goodbye to the menopausal ovary.


Thyroid-stimulating hormone and prostaglandin E1 stimulation of cyclic 3',5'-adenosine monophosphate in thyroid slices. Thyroid-stimulating hormone increased the cyclic 3',5'-adenosine monophosphate concentration in dog thyroid slices during a 1-minute incubation period and produced a maximum effect soon thereafter. The elevation persisted for at least 30 minutes. The concentrations of the cyclic 3',5'-adenosine monophosphate increased as the TSH concentration was increased from 0.125 to 50 milliunits per milliliter. Prostaglandin E(1), which increases glucose oxidation in dog thyroid slices, also increased the concentration of cyclic 3',5'-adenosine monophosphate. Although sodium fluoride stimulates thyroid adenyl cyclase, it did not increase concentration of cyclic 3',5'-adenosine monophosphate. Carbamylcholine and menadiol sodium diphosphate augment glucose oxidation in dog thyroid slices but do not change concentrations of cyclic 3',5'-adenosine monophosphate.


Neutralization kinetics of poliovirus by specific antiserum during the course of immunization of rabbits.


Microscopic skin lesions in cattle with foot-and-mouth disease.


Thermostability of cytomegalovirus strains.


The cellular site of virus replication in the intestine of chicks infected with an avian adenovirus.


Rct-marker tests on isolates from WM3 and Sabin type 3 attenuated poliovirus vaccines.


Detection of polioviruses in human cerebrospinal fluid.


Decreased insulin growth dependence in transformed hamster cells.


Studies on the biochemistry and fine structure of silica shell formation in diatoms. IV. Fine structure of the apochlorotic diatom Nitzschia alba Lewin and Lewin.


Thermostimulation of conidiation and succinic oxidative metabolism of neurospora crassa.


On the metabolism of a myo-inositol-mannoside in Saccharomyces cerevisiae.


Post-vaccination reactions in donkeys vaccinated with African horse-sickness polyvalent vaccines.


Occurrence and survival of foot-and-mouth disease virus in external lesions and discharges of experimentally infected buffalo-calves.


Excretion of fowl plague virus by immune and non-immune fowls.


Attenuation of foot-and-mouth disease virus type "O" by serial passages in goats.


Excretion of individual unconjugated 17-oxosteroids by normal female subjects.


New types of respiratory deficient mutants in Saccharomyces cerevisiae. I. A segregational mutant with ineffective respiration.


Some biologic and metabolic properties of the 186th passage of mouse chrysoidin hepatoma.


Rapid plasma cortisol method using micro-columns.


Preliminary characterization of the collagenolytic activity of rat bone.


Neuroglia proliferation studies in tissue culture.


The influence of age on infection in experimental Herpes simplex encephalo-myelo-neuritis of mice as demonstrated by fluorescent antibody method.


The effect of estrogen on intravenous glucose tolerance in woman.


Effect of trace metals on placental metabolism.


Studies of Burkitt lymphoma cells. 3. Equilibrium density gradient centrigugation of virus particles isolated from Burkitt lymphoma cell lines.


Palpable axillary lymph nodes in cancer of the breast. Structural and biologic considerations.


Preoperative irradiation of cancer of the lung. Preliminary report of a therapeutic trial. A collaborative study.


Histologic effects of endolymphatic radiotherapy.


Indirect thyroid lymphography in the dog using radio-iodinated lipiodol ultra fluid.


Effects of necrotic tumor on hepatic cells in the rat. An ultrastructural study.


Glomangioma. An ultrastructural study.

Glomangioma. An ultrastructural study.


Neoplasms of the nervous system in Thailand.


Localization and characteristics of the cortical waves associated with eye movement in the cat.


Macroelectrode responses with stimulation and recording within hippocampus and related structures during different states of consciousness.


Induction of both emotional behavior and a novel form of REM sleep by chemical stimulation applied to cat mesencephalon.


Hepatocellular carcinoma in a fowl.

Hepatocellular carcinoma in a fowl.


Some determinants of the effects of VAL-5-angiotensin II amide on glomerular filtration rate and sodium excretion in dogs. In 12 dogs anesthetized with chloralose, angiotensin (angiotensin II amide) given intravenously increased the glomerular filtration rate (GFR) of an ischemic kidney while simultaneously having little effect on the GFR of the contralateral kidney. In the ischemic kidney, in 14 of 30 observations, increments of GFR greater than 100% of mean control GFR (9 ml/min) occurred in response to angiotensin. The magnitude of the increase in GFR produced by angiotensin was independent of dose (range 0.005-0.050 mug/kg per min), the degree of accompanying pressor response, and alterations in renal blood flow (RBF) (electromagnetic flow-meter). In the ischemic kidney, increments of GFR could be produced by sub-pressor doses of angiotensin. Dissociations between increments of GFR and sodium excretion occurred. Equivalent increments of GFR in the ischemic kidney in dogs receiving either 5% glucose in water or 10% mannitol in 0.3% saline were associated with natriuresis only in the latter group: a) as an initial response of the contralateral kidney to renal arterial constriction (RAC) in spite of a concomitant reduction in RBF and an unchanged GFR; b) in the ischemic kidney on giving angiotensin. The natriuresis produced by angiotensin was independent of the magnitude of elevations in blood pressure, altered filtration fraction, and was associated with a further reduction in RBF. After release of RAC in the dogs receiving mannitol, an antinatriuresis was again observed in response to angiotensin. The presence of unilateral renal ischemia allowed the demonstration of a differential action of angiotensin on the GFR of an ischemic and nonischemic kidney. The natriuresis in response to angiotensin requires, in addition to mannitol, the participation of undefined factors invoked by unilateral renal ischemia.


Effect of mixed micellar lipid on the absorption of cholesterol and vitamin D3 into lymph. The absorption of endogenous cholesterol, labeled with tracer doses of cholesterol (14)C or cholesterol-(3)H and of near physiological doses of vitamin D(3)-(3)H was studied in rats with cannulated intestinal lymphatics. The effects of administering mixed micellar solutions of fatty acid, monoglyceride, and bile salt on the absorption of these labeled sterols was determined. It was observed that the specific activity of free cholesterol and the amounts of vitamin D(3) appearing in lymph were significantly increased during the intraduodenal administration of mixed micellar solutions of either linoleic or palmitic acid, in contrast to control rats receiving a micellar solution of taurocholate. These increases were related linearly to the lymph triglyceride level. In addition it was observed that when the linoleic acid solution was administered there was a more marked increase in the ratio of the specific activities of free and esterified cholesterol in lymph than with either the palmitic acid or taurocholate solutions. Additional studies in rats with intact lymphatics showed that the uptake of labeled cholesterol and vitamin D(3) from the intestinal lumen into the wall was similar whether the sterols were administered in taurocholate or in mixed micellar solution. These findings suggest that mixed micellar lipid increased the rate of appearance of labeled free cholesterol and vitamin D(3) in lymph by enhancing their transport out of the intestinal mucosa, rather than by an effect on uptake.


Cytophotometric quantitation of glucose 6-phosphatase activity in rat liver.


Activation of aldosterone secretion in primary aldosteronism. Angiotensin infusion evokes marked increases in aldosterone secretion in primary aldosteronism and little change in secondary aldosteronism. The low plasma renin activity of primary aldosteronism and the elevated plasma renin activity of secondary aldosteronism are thought to account for this differential response. The effect of angiotensin on aldosterone and 18-hydroxycorticosterone secretion was studied during adrenal vein catheterization in seven patients with primary aldosteronism (whose plasma renin activity had been elevated following spironolactone therapy), one hypertensive patient with normal plasma renin activity and normal aldosterone secretion, two patients with secondary aldosteronism who had elevated plasma renin activity, and one anephric patient whose plasma renin activity was 0. Adrenal venous aldosterone and 18-hydroxycorticosterone were measured before and after a ten min sub-pressor angiotensin infusion. The cells of the aldosterone-producing adenoma (APA) respond to small increases in plasma angiotensin with large increases in secretion of aldosterone and 18-hydroxycorticosterone. The dose of angiotensin capable of evoking this response from the aldosterone-producing adenoma produces little or no change in the secretion of the steroids from nontumorous glands. The augmentation of aldosterone secretion, induced by angiotensin, in primary aldosteronism is due solely to increased secretion by the adenoma and not by the contralateral zona glomerulosa. The increased sensitivity of the aldosterone-producing adenoma is characteristic of the tumor. This response is independent of fluctuations in endogenous plasma renin activity. This sensitivity is not blunted by high plasma renin activity, nor is it a function of tumor mass for the effect is observed in aldosterone-producing adenomas regardless of size. ACTH injection after angiotensin infusion resulted in a marked increase in aldosterone concentration in the effluent from the nontumorous adrenal, but was not capable of producing further increases in aldosterone concentration in the effluent from the APA. In view of this exquisite sensitivity to infused angiotensin, it may be that the small variations in endogenous plasma renin activity that have been observed in primary aldosteronism may be capable of evoking large changes in aldosterone secretion in patients with aldosterone-producing adenomas.


Correlation between the neutralizing activity of human serum against Herpes simplex virus and a simian herpesvirus (SA8).


Effect of intranasal reovirus infection on antibacterial activity of mouse lung.


A system of tissue cultures for the study of a mouse leukemia virus.


Spread of enteric live adenovirus type 4 vaccine in married couples.


Studies of a newly recognized poxvirus of monkeys.


The fibre size and content of the radial and sural nerves.


Thalidomide neuropathy: a clinical electrophysiological, and histological follow-up study.