Primitive reflex activity in primary and symptomatic Parkinsonism.


Globular neuropathy. A disorder of axons and Schwann cells.


Histochemistry of Rathke pouch tumours.

Histochemistry of Rathke pouch tumours.


Cytochemistry of sea urchin gametes. I. Intramitochondrial localization of glycogen, glucose-6-phosphatase, and adenosine triphosphatase activity in spermatozoa of Paracentrotus lividus.


Effect of BCG and allogeneic tumor cells on adenovirus type 12 tumorigenesis in mice.


Relationship between virion incidence and pathological stage in virus-induced leukaemia.


Nuclear sheets in epithelial and connective tissue cells.


Inhibition of gastric acid secretion in vitro by prostaglandin E1.


Central effect of the secondary endings of muscle stretch receptors in man.


Neurological diagnosis of ruptured cervical discs.


Gas-liquid chromatographic analysis of sural nerves in peripheral neuropathies.


Femoral neuropathy with abdominal pain.

Femoral neuropathy with abdominal pain.


Poliovirus crystals within the endoplasmic reticulum of endothelial and mononuclear cells in the monkey spinal cord. The lumbar motor columns of a cynomolgus monkey that had become tetraplegic after experimental infection with a highly virulent strain of type 3 poliovirus were examined by electron microscopy. Crystalline aggregates of poliovirus occurred within the endoplasmic reticulum of endothelial cells as well as of mononuclear inflammatory cells. This finding suggests that the endoplasmic reticulum might be much more involved in poliovirus multiplication than has been previously supposed.


Venesection therapy in idiopathic haemochromatosis. An analysis of 40 treated and 18 untreated patients.


Incidence of thrombosis in portal venous stasis and hypertension.


Nerve involvement in leprosy. Pathogenesis and significance.


Sporotrichosis and nocardiosis in a patient with Boeck's sarcoid.


Fetal hamartoma of kidney. A benign, symptomatic, congenital tumor, not a form of Wilms' tumor.


Antiviral effectiveness of chlorine bleach in household laundry use.


Susceptibility of Bordetella pertussis to nine antimicrobial agents.


The enzymic conversions of 13-hydroperoxy-cis-9-trans-11-octadecadienoic acid into 13-hydroxy-12-oxo-cis-9-octadecenoic acid.


Investigation of human malignant tumors in India for simian virus 40 etiology.


Multiplication of avian leukosis virus in endocrine organs of congenitally infected chickens.


Etiology of Burkitt's lymphoma--an alternative hypothesis to a vectored virus.


A search for avian leukosis virus and antiviral activity in the blood of leukemic and nonleukemic adults and children.


Energy expenditure and nitrogen metabolism of rats bearing a transplantable anterior pituitary tumor.


Ultrastructure of Wilms' tumor. I. Epithelial cell.


Anthelmintics, new and old.

Anthelmintics, new and old.


Differentiation of pseudoinflammatory colloid carcinoma from colitis cystica profunda.


Conductance changes during pyramidally induced postsynaptic potentials in red nucleus neurons.


Comparative study of acetylcholine and 5-hydroxytryptamine receptors on single snail neurons.


Slow inhibitory and excitatory postsynaptic responses in single cells of mammalian sympathetic ganglia.


Radioisotopes in malignancies in children. Use in the study of involvement by primary and metastatic processes in brain and liver.


Glomus jugulare tumor.

Glomus jugulare tumor.


The neurological complications of ankylosing spondylitis.


The recorded body-sway response to galvanic stimulation of the labyrinth: a preliminary study.


Changes in surface antigens of SV40-virus transformed cells.


Subacute "poliomyelitis" and Hodgkin's disease.


The effect of exogenous dopamine on ACTH secretion.


Skin test potency in guinea pigs of Entamoeba histolytica antigen (histolyticin) prepared from axenically cultivated amebas.


Adrenal cortical control of adrenal medullary function.


Adrenergic receptor blockers on pressor activity of incubated human plasma.


Effect of cycloheximide on the antiviral action of interferon.


Viral inhibition of the phytohemagglutinin response of human lymphocytes and application to viral hepatitis.


Giant fibroadenoma (benign cystosarcoma phylloides): report of case in 13-year-old girl.


Immunology of mouse mammary tumor virus.


Portal diversion in glycogen storage disease.


The effects of vasoactive drugs on reticuloendothelial function in experimental shock and combined injury.


Ethambytol in the retreatment of pulmonary tuberculosis. United States Public Health Service cooperative trial.


The treatment of drug resistant chronic pulmonary tuberculosis with new tuberculostatics.


The clostridial flora of marine sediments from a productive and from a non-productive area.


An immunologic comparison of five murine leukemia viruses grown in tissue culture.


Serological characterization of gamma-M(19 S) and gamma-G(7 S) rabbit antibiodies in response to Entamoeba histolytica antigens.


Quantitative immunochemical determination of the isozymes of aspartate aminotransferase in rat livers and transplantable rat hepatomas.


Changes in virus attachment and other surface properties of L4946 mouse leukemia cells on adaptation of growth in tissue culture.


The effect of mumps virus on the resistance of Burkitt lymphoma cell lines to various viruses.


Ultrastructure of chick embryo cells altered by strain MC29 avian leukosis virus.


Effect of herpes simplex virus on cultured Burkitt tumor cells and its failure to influence the Epstein-Barr virus carrier state.


Deep spreading carcinoma of the stomach causing death within seven months of last normal x-ray examination.


Fine structure of the pars distalis and interrenals of Taricha torosa after administration of metopirone (SU-4885).


Physiological evidence for a pituitary-adrenocortical feedback mechanism in the eel (Anguilla rostrata).


Interconversions of estrogens and related developmental effects in sand dollar eggs.


Preparation of rabbit immune serum with neutralizing activity against a simian cytomegalovirus (SA6).


Poliovirus antibody response in serum and nasal secretions following intranasal inoculation with inactivated poliovaccine.


Immune response of cattle to different serotype antigens in monovalent and trivalent foot-and-mouth disease vaccines.


Mammary tumor virus potentiation of endogenous prolactin effect on mammary gland differentiation.


Demonstration of tumor-specific immunity against antigens unrelated to the mammary tumor virus in spontaneous mammary adenocarcinomas.


Response of hyperplastic alveolar nodule outgrowth-line D1 to mammary tumor virus, nodule-inducing virus, and prolonged hormonal stimulation acting singly and in combination.


Acquired immunological tolerance and carcinogenesis by the mammary tumor virus. II. Immune responses influencing growth of spontaneous mammary adenocarcinomas.


Acquired immunological tolerance and carcinogenesis by the mammary tumor virus. I. Influence of neonatal infection with the mammary tumor virus on the growth of spontaneous mammary adenocarcinomas.


Nephroblastoma (Wilms tumor) and congenital aniridia.


Spinal cord representation of the peripheral neural pathways of the micturition reflex.


The action of staphylococcal alpha toxin and the venom from Bitis gabonica on rat stomach strip.


Electron microscopic study of isolated Kupffer cells.


Analysis of excitation process in squid giant axons under bi-ionic conditions.


Inactivation of angiotensin in shock.

Inactivation of angiotensin in shock.


Plasma renin activity in hypertensive rats after treatment with renin preinhibitor.


Influence of glycolysis on NADH content in human erythrocytes.


Renal extraction of PAH, GFR, and UNaV in the rat during infusion of angiotensin.


Effects of gallamine and physostigmine on transmission through the cuneate nucleus.


Antagonism of angiotensin by hydralazine on renal blood flow and erythropoietin production.


Caffeine effects on transmitter depletion and mobilization at motor nerve terminals.


Influence of cycling, pregnancy, labor, and suckling on corticosterone-ACTH levels.


Hyperbaria in experimental bile peritonitis.


Spontaneous mammary adenocarcinoma in mice: influence of thymectomy and reconstitution with thymus grafts or spleen cells.


Regulatory properties of a plant NAD: isocitrate dehydrogenase. The effect of inorganic ions.


Malate dehydrogenases of ox kidney. 2. Two substrate kinetic and inhibition analyses.


The reversible removal of one specific copper(II) from fungal laccase.


The substrate specificity of pigeon liver NAD kinase.


The effect of temperature on ATP levels in cells of an ascitic hepatoma.


The oxidation of exogenous and endogenous cytochromeC in mitochondria. A biochemical and ultrastructural study. The effect of the nonionic detergent Lubrol on the oxidation of endogenous and exogenous cytochrome c by cytochrome oxidase in intact and fragmented mitochondria was studied. Mitochondria and mitochondrial fragments from liver, kidney, heart, and skeletal muscle have been used. Negatively stained preparations of intact mitochondria showed the particles of Fernández-Morán on the matrix side of their inner membrane system: under these conditions, the oxidation rate of externally added cytochrome c was very high, and it was stimulated very poorly by Lubrol. Mechanical fragmentation of liver mitochondria yielded vesicles with a smooth external profile: also under these conditions, the oxidation of externally added cytochrome c was very high, and poorly stimulated by Lubrol. The oxidation of endogenous cytochrome c was also unaffected by Lubrol. On the other hand, fragmentation of heart and skeletal muscle mitochondria yielded vesicles having numerous particles of Fernández-Morán on their external profiles. Under these conditions, the oxidation of exogenous cytochrome c was low and was markedly stimulated by Lubrol. On the contrary, no activation of the oxidation of endogenous cytochrome c was induced by the detergent. The results indicate a difference in the permeability properties of the two faces of the inner mitochondrial membrane: a permeability barrier for cytochrome c is suggested to exist at the inner face.


Studies on the inhibitory properties of sodium thioglycollate on the germination of wet spores of clostridia.


Glomangioma of external ear.

Glomangioma of external ear.


The effect of local blockage of motor nerve terminals. 1. Electrophoretic application of tetrodotoxin (TTX) was used to study the effect of localized blockage of impulses in motor nerve terminals.2. Under suitable conditions it can be shown that local production of end-plate potentials ceases distal to the site of TTX application, while it continues without diminution at proximal sites of the same terminal arborization.3. It is concluded that electrotonic spread of an action potential wave along a motor nerve terminal is insufficient to elicit transmitter release. Active propagation in the terminals is an essential requirement for neuromuscular transmission.


The re-distribution of cytochrome oxidase, noradrenaline and adenosine triphosphate in adrenergic nerves constricted at two points. 1. The experiments correlate certain changes in the ultrastructure of cat hypogastric nerves constricted at two points with the distribution of a mitochondrial enzyme (cytochrome oxidase), noradrenaline (stored in some of the vesicles with an electron dense core, i.e. granular vesicles) and adenosine triphosphate (ATP) (present in noradrenaline storage granules, mitochondria and the soluble fraction of the axon).2. Noradrenaline (NA) and granular vesicles accumulated proximal but not distal to both constrictions. The total amount of NA and the concentration of granular vesicles above the first constriction was greater than that present in a similar piece of normal nerve, indicating that the cell body was continuing to produce the transmitter despite injury to its axon. The granular vesicles proximal to the first constriction were found in swollen or distorted axons and in new axonal outgrowths. It was concluded that the movement of NA in these constricted nerves was only centrifugal in direction.3. Mitochondria and cytochrome oxidase accumulated on both sides of the two constrictions, indicating a bi-directional movement of mitochondria in the damaged axons. The possibility that some of the increase in the cytochrome oxidase could be related to an increase in the number of mitochondria in cells other than neurones is considered.4. The adenosine triphosphate content increased on both sides of the two constrictions. This increase developed more slowly and was less marked than that of the other two substances.5. It was concluded that (a) there was a close correlation between the behaviour of noradrenaline and granular vesicles and between cytochrome oxidase and mitochondria, (b) the dense cored vesicles and the mitochondria moved independently of one another and at different rates after constriction of non-myelinated axons, (c) while some of the changes may be attributed to an obstruction to the free movement of axoplasm others may be due to an active reaction to axonal injury, and (d) localized intraaxonal synthesis of noradrenaline and cytochrome oxidase did not occur between the two constrictions.


Colostomy and its management.

Colostomy and its management.


Adrenal secretion rates and adrenal tissue concentrations of pregnenolone, progesterone, 11 beta OH-androstenedione and some other steroids in young pigs and dogs. 1. The rates were measured at which a number of different steroids were secreted by the adrenal gland of the eviscerated and nephrectomized young pig and dog.2. In addition to cortisol and corticosterone the following steroids were consistently found to be present in the adrenal venous blood: pregnenolone, progesterone, 11betaOH-progesterone, androstenedione, 11betaOH-androstenedione and adrenosterone.3. The sum of the latter steroids could amount to as much as 30% of the total steroid secretion.4. A severe deficit in the blood volume increased the secretion of pregnenolone and the 17-oxo steroids in the pig.5. alpha-ethyltryptamine failed to inhibit ACTH release if the animals were eviscerated and nephrectomized, or if they were anaesthetized with chloralose instead of pentobarbitone sodium.6. The same steroids as in the adrenal venous blood were found in extracts from the adrenal glands of a number of species.7. The amount of individual steroids present in the adrenal gland of the stressed pig and dog was compared with the rate at which each had been secreted immediately before the excision of the gland.8. There was a positive correlation between the adrenal concentrations and the secretion rates of cortisol, the major glucocorticoid secreted by the pig and the dog.9. In contrast, the rate at which pregnenolone was secreted did not show a consistent relationship to its concentration in the gland.10. A storage mechanism for pregnenolone in the adrenal cortex is proposed.


Urinary excretion of polymyxin B sulfate and sodium colistimethate in dogs.


Angiographic findings in 16 patients with juvenile nasopharyngeal angiofibroma.


A new method for radiographic visualization of the bronchopulmonary lymphatic system.


Angiographic features of cavernous hemangioma of liver.


Rhinovirus infections in an industrial population. IV. Infections within families of employees during two fall peaks of respiratory illness.


Salt-losers and non salt-losers in congenital adrenal hyperplasia.


The effects of fever on sleep and dream patterns.


Glycerylphosphorylcholine diesterase: effects of dietary choline deficiency.


Simple method for isolation of crude mono- and digalactosyl diglycerides from wheat flour.


Metabolic effects of imidazole in adipose tissue.


Immunization of day-old chicks against fowl pox.


Action of strychnine on the specific somatosensory projection pathway.


Some pharmacological effects of halothane in fowls.


Thin-layer chromatography of carbohydrates on silica gel impregnated with sodium acetate, monosodium phosphate and disodium phosphate.


The role of elution solvent in quenching of Ittrich estrogen fluorescence reaction observed with silica gel eluates.


Separation of mental-EDTA complexes by thin-layer chromatography.


The regulation of aldosterone production in normal and sodium-deficient rats.


A longitudinal study of the urinary excretion of individual steroids in children from 8 to 12 years old.


Failure of stilboestrol to stimulate corticosteroid excretion in hypophysectomized guinea-pigs.


A simple method for the separate estimation of 11-deoxy and 11-oxygenated 17-hydroxycortico-steroids in human urine.


Genetic marker studies on poliovirus type 1 strains from the Blackburn poliomyelitis outbreak in 1965.


Vaccination with live type 4 adenovirus: evaluation of antibody response and protective efficacy.


Sero-epidemiological study of reovirus infection amongst the normal population of the Chandigarh area--northern India.


Bacteriological aspects of air-conditioning plants.


The persistence of foot-and mouth disease virus in sheep.


Relationship of electrical activity in pontine reticular formation and lateral geniculate body to rapid eye movements.


Experiences in laboratory diagnosis of adenovirus infections in routine medical practice.


Studies on the toxicity of chloroform on the isolated rabbit heart under different conditions of coronary circulation.


Changes in distal sodium reabsorption during angiotensin infusion in response to furosemide.


Transmission of mouse leukemia by oral route.


Rhabdomyosarcohepatoma in an adult and embryonal hepatoma in a child.


Asymptomatic solitary pulmonary nodule. A study of resected cases.


Enzymatic changes in asphyxia, experimental pulmonary edema, and drowning.


Gas-liquid chromatographic studies of urinary 17-ketosteroids in three patients with adrenocorticotropin-producing bronchogenic carcinoma.


Pathogenesis of hypoglycemia in hamsters with a transplantable islet-cell tumor.


The rat as experimental model in sequential radiologic study of the thoracic duct.


Studies on the role of adenylcyclase in insulin secretion.


Hepatoblastoma and hepatoma in infancy and childhood. Light and electron microscopic studies.


Role of free fatty acids in glucose homeostasis.


Femoral compression neuropathy in hemorrhagic disorders.


The use of guanidine thiocyanate to fractionate DNA-like RNA adsorbed on methylated bovine albumin-kieselguhr columns.


Some properties of ribonucleoprotein particles in the isolated nucleoli of mouse ascites tumor cells.


Tertiary structure determinants in transfer RNA. I. Pseudouridine.


Primary infection of herpes simplex causing necrotic cervicitis.


Studies on glutamic oxalacetic transaminase in the rat. 3. Hormonal control of isoenzyme levels in liver by the pituitary-adrenal system.


Three different midpoint oxidation-reduction potentials of cytochrome oxidase from bovine heart muscle.


Biochemistry of shellfish lipids. IX. Enzymatic hydrolysis of ceramide 2-aminoethylphosphonate and sphingoethanolamine.


Radiological differentiation between Wilm's tumour and neuroblastoma in abdominal masses.


Cytomegalic inclusion disease.

Cytomegalic inclusion disease.


Separation of toad venom constituents on silica gel impregnated glass filter paper.


EAN as a model for idiopathic polyneuritis.


Neurofibrillary degeneration induced by Vinca alkaloids: clinical and experimental.


Presynaptic neuromuscular inhibition by delta-aminolevulinic acid, a porphyrin precursor.


Reversal of sleep-waking cycle: effect on sleep stage pattern and certain neuroendocrine rhythms.


Hereditary neuropathy manifested by pressure palsies--a Schwann cell disorder?


Polyneuropathy in the osteosclerotic form of multiple myeloma.


Intranuclear inclusions in bovine embryo cell cultures infected with bovine virus diarrhoea-mucosal disease virus.


Biochemistry of functional neural crest tumors.


A semiautomated method which does not require digestion for the determination of serum thyroxine iodine (T4).


Demonstration of glucagon in islet cell adenomas of the pancreas by immunofluorescent technic.


Is conservative therapy ever justified in stage I (IA) cancer of the ovary?


Somatic sensation.

Somatic sensation.


The endocrine function of the pancreas.

The endocrine function of the pancreas.


In vitro evaluation of an acidulated phosphate fluoride prophylaxis paste.


Potassium dependent phosphatase in bovine parotid gland.


Tumors of the minor salivary glands. A report of 46 cases.


Pleomorphic lymphoma and gastric adenocarcinoma (collision neoplasm) associated with monoclonal macroglobulinemia and amyloidosis. A case report.


Combination chemotherapy using cyclophosphamide, vincristine, methotrexate and 5-fluorouracil in solid tumors.


Intra-epidermal eccrine poroma: a histochemical and enzyme-histochemical study.


Factors influencing distribution of Ultra Fluid Lipiodol (U.F.L.) following intrathyroid injection.


Adenoid cystic carcinoma (cylindroma) of the trachea: case report with 9-year follow-up.


Gero J: Range of the sympathetic control of the dog femoral artery.


Thyrotropin release in vitro: stimulation by cyclic 3',5'-Adenosine monophosphate.


Activation of skeletal adenyl cyclase by parathyroid hormone in vitro.


Hypercalcemic and hypophosphatemic effects of dibutyryl cyclic AMP in rats after parathyroidectomy.


Comparison of assays for corticotrophin-releasing activity.


Inhibition of lactation and luteal function in postpartum rats by hypothalamic implantation of prolactin.


Red cell agglutination kinetics: a method for automatic recording with the fragiligraph.


On the storage and mobilization of organic resources in the hepatopancreas of a crab (Paratelphusa hydrodromus).


Regional blood flows during desynchronized sleep in the cat.


Pressor response to angiotensin I during cardio-pulmonary bypass.


'Spontaneous' occurrence of Yaba tumor in a monkey colony.


The effect of estrogen and castration on a spontaneous pancreatic islet change in rats.


Antiviral activity of certain substituted purine and pyrimidine nucleosides.


Cranial nerve disease.

Cranial nerve disease.


The chiasmal syndrome.

The chiasmal syndrome.


Investigations on the adrenocortical function of normal dogs.


The production of antibodies to porcine corticotrophin and to Synacthen.


Inhibition of norepinephrine re-uptake by angiotensin in brain.


Hormonal control of glycerolphosphate dehydrogenase in the rat brain.


Reinnervation of spinal cord by cholinergic neurons.


Analysis of slow inhibitory postsynaptic potential of bullfrog sympathetic ganglion.


Cytomegalovirus infection after renal transplantation. Report of a case with viremia and atypical lymphocytosis.


Immunization against Marek's disease using a live attenuated virus.


Corticosteroids and ACTH in the management of the atopic child.


Comparison of the diagnostic accuracy of liver scans, liver function tests and liver biopsies.


Electron microscopy as an aid to the rapid identification of animal viruses.


Transmission of more than one sensory modality-message along a single axon.


Effect of the Finnish sauna-bath on the urinary excretion of 17-OH-corticosteroids and blood eosinophil count in allergic and healthy persons.


A comparative study of sleep parameters during pregnancy.


A screening test for neuromuscular disturbances in adynamia episodica hereditaria. Clinical and electrophysiological study.


Cell cultures of bovine embryonic skin: growth and characterization.


Neutralization of canine herpesvirus by dog and human serums: a survey.


Studies of bladder stone disease in Thailand. X. Effect of orthophosphate and nonfat dry milk supplementations on urine composition.


Functional classification of cell types of the anterior pituitary gland accomplished by electron microscopy.


The metabolism and biological activity of esterified vitamin D in the rat.


Experimental adenovirus infection in calves. Twenty-four dairy calves ranging in age from six to 15 weeks were divided so that 10 were inoculated with bovine adenovirus serotype 1 (BA-1), eight were inoculated with bovine adenovirus serotype 3 (BA-3), and six were inoculated with control tissue culture and fluids.Post-inoculation serum samples from most of the calves presented evidence of adenovirus infection, but none of the animals exhibited signs of respiratory or enteric disease. Histopathological examination of lung tissue was not considered to be specific for adenovirus pneumonia, but was characterized by peribronchial cuffing and slight aspiration pneumonia. BA-1 was not isolated from any of the inoculated calves, and BA-3 was only recovered from the feces of two animals. Reasons for the discrepancy between these results and those of other workers are considered, and the etiology of peribronchial cuffing is briefly discussed.


Effect of ketone bodies and ACTH on the transmethylation reaction (S-adenosyl methionine independent system) in rats. I.


Movement of 131-I rose bengal out of the brain tumor bed.


Influence of the anterior pituitary on the aldosterone secretory response to dietary sodium restriction in the rat.


Chemotherapeutic agents.

Chemotherapeutic agents.


Replication of murine sarcoma virus-Harvey (MSV-H) in tissue cultures of virus-induced sarcomas.


A new DNA-exonuclease in cells infected with herpes virus: partial purification and properties of the enzyme.


Mechanism of plaque inhibition of poliovirus possessing the d marker.


The infectivity of polyoma virus DNA for mouse embryo cells in the presence of diethylaminoethyl-dextran.


Studies on the nature and genetic control of an antigen in normal chick embryos which reacts in the COFAL test.


Anti-viral activity of 5,6-dichloro-1-(2'-deoxy-beta-d-ribofuranosyl)benzimidazole and related derivatives.


Circulating interferon in mice infected with the lactate dehydrogenase-elevating virus.


Inhibitory effect of interferon perparation on the development of foci of altered cells induced in vitro by mouse sarcoma virus.


Mass in right upper quadrant of the abdomen.


The effects of air pollutants on mucociliary clearance.


Melanotic progonoma in an African infant.


An EPR study of live and dead Drosophila adults under aerobic conditions.


Immunobiological relationship of goat pox and sheep pox viruses.


Gas-liquid chromatography of ergocalciferol and cholecalciferol in nanogram quantities.


Some observations on Clostridium perfringens strains isolated in Iran.


Study of the rôle of Clostridia in swine gastroenteritis.


Genetic influence on the development of renal hypertension in parabiotic rats. Evidence for a humoral factor. The effects of several renal manipulations including uninephrectomy, unilateral renal artery constriction, and a combination of these two (Goldblatt procedure) were studied in two strains of rats with opposite constitutional predispositions to experimental hypertension. The protective value of intact renal tissue to protect against hypertension was shown to be genetically determined. The Goldblatt procedure carried out on only one member of a parabiotic pair induced hypertension in this operated rat but significant hypertension developed in the intact partner only when the operated animal belonged to the strain predisposed to hypertension. It was speculated that there were qualitative differences in the pressor signals of the two strains of rats. In the strain genetically predisposed to hypertension there are at least two pressor principles: (a) one which is common to both strains, not transmittable via the parabiosis junction and presumably related to the renin-angiotensin system; and (b) a second which is specific for the hypertension-prone strain and can be transmitted through the parabiosis junction. This transmittable agent is probably identical with the factor that produces salt hypertension and is associated with the salt-excreting mechanism.


Intestinal Clostridium perfringens in arthritis and parakeratosis induced by dietary factors. Experimental studies in pigs.


Typing of the foot-and-mouth disease virus. Results of diagnosis and typing of foot-and-mouth disease virus.


The occurrence and properties of leukocytosis and lymphocytosis-stimulating material in the supernatant fluids of Bordetella pertussis cultures. 1. Leukocytosis- and lymphocytosis-stimulating activity was present in fluid cultures of B. pertussis. The activity was found primarily in the culture supernatant fluid. 2. The sequential changes in the leukocyte response were similar to those previously observed following injection of intact bacteria into mice. 3. Activity was destroyed by heat and was diminished, but not abolished, by prolonged treatment with proteolytic enzymes. 4. A water-insoluble fraction of the culture supernatant fluid was isolated which contained virtually all of the activity. The specific activity was more than 100-fold greater than that of the intact bacteria, and injection of microgram quantities produced a response. 5. The distribution of histamine-sensitizing factor followed that of leukocytosis-stimulating activity. In contrast, mouse protective antigen was localized to the bacterial pellet.


Studies on the entry of viruses into the central nervous system of mice via the circulation. Differential effects of vasoactive amines and CO2 on virus infectivity. The inhalation of CO(2) exerted a selective action on the entry of viruses into the CNS of mice via the circulation. In 6 wk old animals, the infectivity of poliovirus, vaccinia, and vesicular stomatitis viruses was enhanced, whereas the entry of herpesviruses and of a neurotropic influenza virus was not affected. However, in 3 to 4 wk old mice, inhalation of the gas enhanced the infectivity of all the viruses. To explain the relationship of age to the animals' response to CO(2), it was proposed that CO(2) inhalation, by increasing the rate of cerebral blood flow, served to increase the exposure of some extraneural cell(s) to virus, possibly the cerebrovascular endothelial cell, in which virus replicated and reached the brain parenchyma by direct extension of the infectious process. Consequent to maturation, the postulated target cell developed resistance to certain viruses, while its susceptibility to others remained unchanged. Inoculation of adrenalin or serotonin simultaneously with virus into the circulation enhanced the CNS infectivity of all the viruses tested in both 4 wk and 6 wk age groups of mice. The enhancing effects were completely inhibited by the action of an alpha-adrenergic blocking agent; it was therefore concluded that hemodynamic changes produced by the contractile response of the smooth muscle cells in the vessel walls mediated the enhancing effect. It was suggested that a vasopressor response invoked in the peripheral vessels, with a consequent increase in cerebral blood flow, increased virus dosage to the CNS. In addition, a direct effect of the vasoactive amine on the cerebral vessels, resulting in the disruption of an anatomical "barrier", was considered as a contributory factor in increasing the transfer of virus to the CNS from the circulation.


Peripheral nerve syndromes.

Peripheral nerve syndromes.


Forensic medicine.

Forensic medicine.


Typing of the foot-and-mouth disease virus. Cumulative Quarterly report.


The occurrence in Denmark of necrotizing enteritis in piglets caused by Clostridium perfringens type C.


Eye movement ativity during sleep and intellectual function in mental retardation. A positive relation was found between the amount of eye movement during rapid-eye-movement or paradoxical sleep and estimates of intellectual level in a group of retarded adults. This result supports the hypothesis that during sleep the brain carries out processes important for cognitive function.


Effects of vasoactive agents on intestinal blood flow and motility in the dog.


Preventive psychiatry for the family: theory, approaches, and programs.


Glioblastoma multiforme: its treatment and some factors effecting survival.


Antagonistic actions between dibutyryl adenosine-3',5'-cyclic monophosphate and insulin on the metabolism of the surviving rat diaphragm.


The role of anticodon in the acceptor function of tRNA.


Influence of metal ions on the orthophosphatase and inorganic pyrophosphatase activities of human alkaline phosphatase.


A product-inhibition study of the mechanism of mitochondrial octanoly-coenzyme A synthetase. By a study of the product-inhibition kinetics of the octanoyl-CoA synthetase from ox liver mitochondria, evidence was obtained consistent with the hypothesis that the enzyme reacts by a Bi Uni Uni Bi Ping Pong type of mechanism in which the order of addition and evolution of substrates and products is CoA, octanoate, octanoyl-CoA, ATP, PP(i) and AMP. There is also evidence that more than one molecule of CoA can add to the enzyme and that it may act as an allosteric activator.


Synthesis of 10,11-dihydrofarnesyl pyrophosphate from 6,7-dihydrogeranyl pyrophosphate by prenyltransferase. The syntheses of 6,7-dihydrogeraniol and of its pyrophosphate are described. It is shown that this analogue of geranyl pyrophosphate is a substrate for liver prenyltransferase and that the product synthesized by this enzyme from it and isopentenyl pyrophosphate is 10,11-dihydrofarnesyl pyrophosphate. The K(m) value for 6,7-dihydrogeranyl pyrophosphate was determined to be 1.11+/-0.19mum as compared with 4.34+/-1.71mum for geranyl pyrophosphate. The maximum reaction velocity with the artifical substrate was, however, only about one-fourth of that observed with geranyl pyrophosphate. The binding of isopentenyl pyrophosphate to the enzyme was not affected by the artificial substrate.


Temperature and the regulation of enzyme activity in poikilotherms. Properties of rainbow-trout fructose diphosphatase. 1. The properties of fructose diphosphatase from the liver of rainbow trout (Salmo gairdnerii) were examined over the physiological temperature range of the organism. 2. Saturation curves for substrate (fructose 1,6-diphosphate) and a cofactor (Mg(2+)) are sigmoidal, and Hill plots of the results suggest a minimum of two interacting fructose 1,6-diphosphate sites and two interacting Mg(2+) sites per molecule of enzyme. 3. Mn(2+)-saturation curves are hyperbolic, and the K(a) for Mn(2+), which inhibits the enzyme at high concentrations, is 50-100-fold lower than the K(a) for Mg(2+). 4. Fructose diphosphatase is inhibited by low concentrations of AMP; this inhibition appears to be decreased and reversed by increasing the concentrations of Mg(2+) and Mn(2+). Higher concentrations of AMP are required to inhibit the trout fructose diphosphatase in the presence of Mn(2+). 5. The affinities of fructose diphosphatase for fructose diphosphate and Mn(2+) appear to be temperature-independent, whereas the affinities for Mg(2+) and AMP are highly temperature-dependent. 6. The pH optimum of the enzyme depends on the concentrations of Mg(2+) and Mn(2+). In addition, pH determines the K(a) for Mg(2+); at high pH, K(a) for Mg(2+) is lowered. 7. The enzyme is inhibited by Ca(2+) and Zn(2+), and the inhibition is competitive with respect to both cations. 8. The possible roles of these ions and AMP in the modulation of fructose diphosphatase and gluconeogenic activity are discussed in relation to temperature adaptation.


Analogues of geranyl pyrophosphate as inhibitors of prenyltransferase. Six analogues of geranyl pyrophosphate (the monophosphates of geraniol and tetrahydrogeraniol, and the pyrophosphates of nerol, octan-1-ol, tetrahydrogeraniol and citronellol) were synthesized, and were found to be inhibitors of pig liver prenyl- (geranyl-)transferase. The effects of each analogue were analysed in kinetic experiments, which showed the pyrophosphates of citronellol, tetrahydrogeraniol and octan-1-ol to be the most potent inhibitors. The results are interpreted to support a previous hypothesis that the main forces in the binding of substrates to prenyltransferase are non-specific lipophilic forces and a pyrophosphate-binding force.


A comparison of the properties of the phosphofructokinases of the fat body and flight muscle of the adult male desert locust. 1. Phosphofructokinase was isolated, and partially purified by ammonium sulphate fractionation, from the fat body and flight muscle of the desert locust. 2. Ammonium sulphate appears to stabilize the enzymes, but does not activate them. 3. Both flight-muscle and fat-body enzymes give sigmoidal hexose monophosphate concentration-activity curves, which are characteristic of regulatory enzymes. 4. At low ATP concentrations both the enzyme activities increase rapidly with increasing ATP concentrations, but above an optimum concentration ATP becomes inhibitory. This optimum concentration is 0.2mm for the fat-body enzyme and 0.1mm for the flight-muscle enzyme. 5. AMP activates both the enzymes; half-maximal activation occurs at 10mum in each case, the effect being independent of substrate concentration. 6. 3',5'-(cyclic)-AMP (0.5mm) and P(i) (1mm) activate the flight-muscle enzyme, but have no effect on the fat-body enzyme. 7. FDP (1mm) inhibits both enzymes, and with the flight-muscle enzyme this inhibition is increased by increasing the ATP concentration. 8. Citrate, phosphoenolpyruvate and alpha-glycerophosphate have no effect on either enzyme under the assay conditions used. 9. The properties of phosphofructokinases from the locust are compared with those of phosphofructokinases from other sources.


Systemic corticosteroids and folic acid antagonists in the treatment of generalized pustular psoriasis. Evaluation and prognosis based on the study of 104 cases.


Afferent respiratory pathways in the avian vagus.


Pyruvate metabolism in peripheral neuropathy.


Age determination of bone fragments.

Age determination of bone fragments.


Essential fatty acid dificiency and the testis: lipid composition and the effect of preweaning diet.


Hematin formation and pancreatitis.

Hematin formation and pancreatitis.


Detailed analysis of neuromuscular transmission in a patient with the myasthenic syndrome sometimes associated with bronchogenic carcinoma.


Degradation of (131 I) insulin and inhibition of post-heparin lipolytic activity by collagenase.


Clinicopathologic conference. Renal insufficiency following renal transplant. Résumé of case.


Evaluation of local anaesthetics in man. Studies on the blocking effects of some local anaesthetics and their late effects on nerve transmission.


Regulation of peripheral circulation in anaesthesia.


Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients. VII. The isoelectric spectra of fungal laccase A and B.


Conversion of alpha-L-angiotensin amide to beta-L-angiotensin during enzymatic degradation.


Double blind evaluation of the haemostatic effect of adrenochrome monosemicarbazone, conjugated oestrogens and epsilonaminocaproic acid after adenotonsillectomy.


Radiometric method for determination of glucose 6-phosphatase activity in liver.


Vitiligo. HepATOMA.

Vitiligo. HepATOMA.


Determination of enzymatic activity in polyacrylamide gels. I. Enzymes catalyzing the conversion of nonreducing substrates to reducing products.


Tumor response to phenylalanine-tyrosine-limited diets.


Glycolysis-dependent exchange of diphosphopyridine nucleotide-3H in red blood cells and ascites cells.


Resolution and reconstitution of the mitochondrial electron transport system. II. Reconstitution of succinoxidase from individual components.


The metabolism of isolated fat cells. 8. Amino acid transport in ghosts.


Human hypoxanthine phosphoribosyltransferase. II. Kinetics and chemical modification.


The estimation of kinetic constants for the lactate dehydrogenase system by the use of integrated rate equations.


Configuration of the ketosidic bond of sialic acid.


Importance of sulfhydryl groups for lipolytic action in the isolated fat cell.


Presence and location of an unusual amino acid, epsilon-N-trimethyllysine, in cytochrome c of wheat germ and Neurospora.


Intraneural lipoma of the median nerve. Report of two cases and review of the literature.


Effect of sodium deficiency on adrenal sensitivity to angiotensin and ACTH in the rat.


Application of the coated charcoal separation method to the radio-immunoassay of plasma corticotrophin.


The stability of endogenous and exogenous corticotrophin in rat plasma as established by a bioassay in intact rats.


The plasma corticotrophin response to insulin hypoglycaemia, lysine-vasopressin and metyrapone in pigs.


Problems in the determination of urinary 1-oxygenated steroids.


The possible role of adenyl cyclase in thyrocalcitonin release.


Active calcium and strontium transport in human erythrocyte ghosts. Both calcium and strontium could be transported actively from erythrocytes if adenosine triphosphate, guanosine triphosphate, or inosine triphosphate were included in the hypotonic medium used to infuse calcium or strontium into the cells. Acetyl phosphate and pyrophosphate were not energy sources for the transport of either ion. Neither calcium nor strontium transport was accompanied by magnesium exchange, and the addition of Mg(++) to the reaction medium in a final concentration of 3.0 mmoles/liter did not promote the transport of either ion. In the absence of nucleotide triphosphates, the addition of 1.5 mmoles/liter of Sr(++) to the reaction solution did not bring about active calcium transport and similarly 1.5 mmoles/liter of Ca(++) did not bring about active strontium transport. The inclusion of 1.5 mmoles/liter of Ca(++) or Sr(++) in the reaction medium did not interfere with the transport of the other ion when the erythrocytes were infused with adenosine triphosphate.


Neonatal canine herpesvirus infection: a review of present knowledge.


Stereotaxic transsphenoidal biopsy and cryosurgery of pituitary tumors.


Decreased glycolytic enzyme activity in epiphyseal cartilage of cortisone-treated rats.


Beta-aspartylhydroxamic acid: its action as a feedback inhibitor and a repressor of asparagine synthetase in Lactobacillus arabinosus.


The effects of dietary protein on the hepatic enzymes of serine metabolism in the rabbit.


Plasma cortisol and response to corticotrophin in airways obstruction.


Today's drugs.

Today's drugs.


Aftercare for mentally abnormal offenders in the Netherlands.


Mechanism of renal hypertension. Utilization of phentolamine (Regitine) test in essential, renal and angiotensin induced hypertension.


Biochemical basis of obligate autotrophy in Nitrosomonas europaea. The specific activities of isocitric dehydrogenase, alpha-ketoglutaric dehydrogenase, succinic dehydrogenase, malic dehydrogenase, and reduced nicotinamide adenine dinucleotide (NADH) oxidase were determined in extracts of Nitrosomonas europaea and compared with the corresponding values for Anacystis nidulans and autotrophically grown Hydrogenomonas eutropha. In common with other obligate autotrophs and in contrast to facultative autotrophs, Nitrosomonas extracts lacked alpha-ketoglutaric dehydrogenase and KCN-sensitive NADH oxidase activity and had low succinic dehydrogenase activity. The Nitrosomonas NADH oxidase appeared to be of the peroxidase type.


Conversion of glucose-1-phosphate to 3-keto-glucose-1-phosphate by cells of Agrobacterium tumefaciens. Incubation of resting cells of Agrobacterium tumefaciens with glucose-1-phosphate resulted in the accumulation of a new sugar phosphate in the suspending medium. Approximately 80% of the glucose-1-phosphate consumed was converted to the new compound, which was identified as alpha-d-ribo-hexopyranosyl-3-ulose-1-phosphate (3-ketoglucose-1-phosphate). Both utilization of glucose-1-phosphate and accumulation of 3-ketoglucose-1-phosphate were inhibited by 2,4-dinitrophenol, polymyxin, and d-glucose, which are inhibitors of the glucoside transport system of this bacterium but are not inhibitors of d-glucoside-3-dehydrogenase, which is the 3-ketoglucose-1-phosphate-forming enzyme. Consequently, it was concluded that glucose-1-phosphate penetrates into intracellular space by means of an active transport system. The glucose-1-phosphate is converted to 3-ketoglucose-1-phosphate by d-glucoside-3-dehydrogenase, and the 3-ketoglucose-1-phosphate formed reaches the extracellular space by passing through the surface layer of the bacterium.


Reduced nicotinamide adenine dinucleotide oxidase and alpha-ketoglutaric dehydrogenase activity by Thiobacillus thiooxidans.


Control of gluconeogenesis in liver. 3. Effects of L-lactate, pyruvate, fructose, glucagon, epinephrine, and adenosine 3',5'-monophosphate on gluconeogenic intermediates in the perfused rat liver.


Coenzyme binding to L-alpha-glycerophosphate dehydrogenase.


Mammalian metabolism of glutaric acid.

Mammalian metabolism of glutaric acid.


The biosynthesis of folic acid. IX. Purification and properties of the enzymes required for the formation of dihydropteroic acid.


The effect of deuterium oxide on the fluorescence of reduced nicotinamide adenine dinucleotide free in solution and in complexes with liver alcohol dehydrogenase.


Biosynthesis of a cell wall glucomannan in mung bean seedlings.


The amino acid composition and other properties of thermostable glyceraldehyde 3-phosphate dehydrogenase from Bacillus stearothermophilus.


The separation and partial characterization of L-histidinol phosphatase and an alkaline phosphatase of Saccharomyces cerevisiae.


Cushing's syndrome and adrenal medullary insufficiency with bilateral adrenal calcification.


The influence of mitomycin C, actinomycin D and ultraviolet light on the replication of the viruses of foot-and-mouth disease and vesicular stomatitis.


Independent action of antidiuretic hormone, theophylline and cyclic 3',5'-adenosine monophosphate on cell membrane permeability in frog skin. 1. The effects of antidiuretic hormone (ADH), theophylline and cyclic 3',5'-adenosine monophosphate (AMP) on membrane potentials in frog skin have been investigated.2. Membrane potentials across the outer and inner facing membranes were recorded in both normal and current clamped skins. In the latter condition active transport of sodium had been abolished by ouabain or metabolic inhibitors, but ionic gradients were maintained by passing current through the skin from the inside.3. ADH increases the potential across the outer facing membranes and reduces the skin resistance. The results are consistent with ADH causing an increase in permeability of the outer facing membranes to sodium ions.4. Theophylline reduces the skin potential by reducing specifically the potential across the outer facing membranes. At the same time the skin resistance is reduced. Theophylline acts by increasing the permeability of the outer facing membranes to chloride ions.5. Cyclic 3',5'-AMP causes a biphasic potential change accompanied by an increase in skin resistance.6. Metabolic inhibitors block the response of the skin to ADH but not to theophylline.7. Separate explanations for the increase in sodium transport by ADH, theophylline and cyclic 3',5'-AMP are discussed. It is not necessary to involve cyclic AMP in order to explain the effects of either ADH or theophylline.


Ultrastructure and function of the chromaffin cells in the superior cervical ganglion of the rat.


Natural herpes simplex infection in the owl monkey (Aotus trivirgatus).


Segmental demyelinization in Dejerine-Sottas disease: light, phase-contrast, and electron microscopic studies.


Experimental hypertrophic neuropathy: change in fascicular area and fiber spectrum after acute crush injury.


An evaluation of the remote effects of cancer on the nervous system.


A retrospective study of sarcoma of the breast and the results of treatment.


Regional cerebral blood flow in tumour patients.


The regional circulation in brain tumors.


Tissue hypoxia: cellular oxygen requirements with special regard to the in vivo pO2 of the brain.


Active center of DNA polymerase.

Active center of DNA polymerase.


25-Hydroxycholecalciferol: stimulation of bone resorption in tissue culture. 25-Hydroxycholecalciferol stimulates release of previously incorporated calcium-45 from fetal rat bones in doses of 0.9 to 27 units per milliliter. This effect cannot be produced by much larger doses of vitamin D(3). Comparison of stimulation of bone resorption by 25-hydroxycholecalciferol and parathyroid hormone reveals similarities with respect to time course, dose-response slope, and inhibition by calcitonin.


Allantoinase: association with amphibian hepatic peroxisomes. Differential and density-gradient centrifugation studies have estaiblished an associatiotn between allaintoinase and peroxisomes from the liver of the frog Rana pipiens. The presence of allantoincase in the peroxisome indicates. a iricolytic function for this orgainelle in the liver of amphibians.


Severe occult juvenile myasthenia gravis responsive to long-term corticosteroid therapy.


Bronchial asthma with adreno-cortical hypofunction.


All-night sleep studies of acute schizophrenics.


The growth of varicella-zoster virus in tissue fragments.


Effect of endotoxin from Serratia marcescens on the permeability of vessels in hepatomas and carrageenin granulomas of rats.


Insulin secretion by the ovine fetus in utero.


Pituitary-adrenal stimulating effect of superior mesenteric venous constriction.


Assay of corticotropin-releasing factors by injection into the anterior pituitary of intact rats.


Effects of thyroxine upon biosynthesis of flavin mononucleotide and flavin adenine dinucleotide.


Plasma corticosteroid response to stress in four species of wild mice.


Stimulation of gluconeogenesis in rat liver slices by epinephrine and glucocorticoids.


Adrenocortical function in monkeys after forebrain removal or pituitary stalk section.


Brief hospital treatment of schizophrenia--early results of a multiple-hospital study.


Amebiasis, a familial disease. I. The food handler as a transmitter of amebiasis.


Ameboma of colon and rectum. A report of 58 cases.


Viruses and neoplasia. The search for viruses in human neoplasms.


Electron microscopy and electron microscopic autoradiography of splenic cell cultures from mice with amyloidosis.


Increased synthesis of hyaluronic acid by a mouse cell line chronically infected with Rauscher leukaemia virus.


Biological effects of radiation-induced free radicals in fern spores.


Simultaneous determination of urinary estriol, pregnanediol, and pregnanolone by gas-liquid chromatography.


A simple nonchromatographic determination of glycerophosphatide in serum.


Altered pathways of urinary corticosteroid excretion in liver disease studied by a clinical laboratory method.


Dynamics of cytopathic effect of Sendai virus with action of N1, N1 anhydrobis (beta-hydroxyethyl (biguanide) ABOB).


Sttudies on pyrocatechase. II. Electron spin resonance and other properties of iron in the active center.


Effect of substrate on the conformation of myosin.


Enzymatic joining of polynucleotides. VI. Activity of a synthetic adenylylated polydeoxynucleotide in the reaction.


Nonpolar interactions of inhibitors with the nicotinamide adenine dinucleotide-binding sites of L-alpha-glycerophosphate dehydrogenase.


Studies on the adrenocorticotropic hormone-activated adenyl cyclase of a functional adrenal tumor.


Influence of organic solutes on the reactions of oxidative phosphorylation.


The role of deoxyribonucleic acid in ribonucleic acid synthesis. XVI. The purification and properties of ribonucleic acid polymerase from yeast: preferential utilization of denatured deoxyribonucleic acid as template.


Digestive activity of lysosomes. 3. The digestion of lipids by extracts of rat liver lysosomes.


Separation of a factor indispensable for coupled phosphorylation from the particulate fraction of Mycobacterium phlei.


Monodehydroascorbic acid-transhydrogenase activity and coenzyme concentrations in tissues of ascorbic acid-deficient and control guinea pigs.


Influence of electrical stimulation and lesion in limbic structure upon biosynthesis of adrenocorticoid in the rabbit.


Influence of corticosterone implantation in limbic structure upon biosynthesis of adrenocortical steroid.


Pituitary ACTH release and avoidance behaviour of rats with cortisol implants in mesencephalic reticular formation and median eminence.


Spleen or large left hepatic lobe?

Spleen or large left hepatic lobe?


Comparison of three rhesus groups for antibody patterns to some viruses: absence of active simian virus 40 transmission in the free-ranging rhesus of Cayo Santiago.


Enzootic sendai infection in laboratory hamsters.


Examination of various avian species for neutralizing antibody and susceptibility to rous sarcoma virus.


Bluetongue in sheep: viral assay and viremia.


Bluetongue in cattle: viremia.

Bluetongue in cattle: viremia.


Modification of foot-and-mouth disease viruses in primary dog kidney cell cultures.


Enzymes of equine erythrocytes: changes during equine infectious anemia.


Some characteristics and developmental aspects of rat uridine diphosphogalactose 4-epimerase.


Malate dehydrogenase in leaf peroxisomes.


Presence in liver of a 3':5'-cyclic AMP stimulated kinase for the I form of UDPG-glycogen glucosyltransferase.


X-ray modification of the catalytic and allosteric functions of fructose-I, 6-diphosphatase.


Studies on rat liver ribonucleases. 3. Further studies on heterogeneity of liver lysosomes--intracellular localization of acid ribonuclease and acid phosphatase in rats of various ages.


Choriocarcinoma and related trophoblastic tumors in women.


A study of malignant tumour of synovial origin.


The histogenesis and clinical significance of acidophilic bodies in various liver diseases.


Biosynthesis and inactivation of the adrenergic transmitter substance.


Physiological aspects of cholinergic and monoaminergic neurones.


Spontaneous activity of single neurones in the hypothalamus of rabbits during sleep and waking. 1. A method is described for recording from single cells in the hypothalamus of unanaesthetized freely moving rabbits. Behaviour, bodily movement, skin and brain temperatures and e.e.g. were monitored.2. Patterns of unit firing during slow sleep, paradoxical sleep and waking were studied in several regions of the hypothalamus, thalamus and in the septum.3. Of the 144 cells analysed from waking to slow sleep, fifty-six (39%) decreased mean firing rates, thirty (21%) increased spike discharges and fifty-eight (40%) showed no marked change. Dorsal hypothalamic and massa intermedia thalamic cells fired in brief high frequency clusters during slow sleep with a characteristic ;bimodal' interspike interval histogram. Waking and paradoxical sleep abolished these cluster discharges with a concomitant change to an ;asymmetric' histogram.4. Of the thirty-two cells observed during the three states of waking, slow sleep and paradoxical sleep, a majority (twenty-five or 78%) showed their highest rates of spontaneous discharge during paradoxical sleep. Discharge rates of cells sometimes changed in the course of paradoxical sleep according to the presence or absence of phasic events such as myoclonic motor activity. Two hypothalmic cells were almost totally arrested during paradoxical sleep.5. Analysis of unit firing rates during spontaneous rises in brain temperature during waking and paradoxical sleep revealed that a majority of the neurones (22/24) changed their discharge rates in relation to behaviour rather than to brain temperature. Two cells did appear to respond specifically to the central thermal stimulus.6. Hypothalamic cells do not behave as a homogeneous population in relation to changes in the state of arousal of the rabbit. Spontaneous changes in cell discharge related to sleep-waking behaviour must be considered in any interpretation of hypothalamic unit activity as related to neuroendocrine or autonomic mechanisms.


Effects of catecholamines on the cholinergic neuromuscular transmission in fish red muscle. 1. The effects of adrenaline and noradrenaline on neuromuscular transmission and the question of whether these catecholamines act on the presynaptic nerve terminal or on the post-synaptic membrane were investigated using the fin muscles of the silver carp.2. Noradrenaline (10(-8)-10(-6) g/ml.) had no effect on membrane potential or input resistance of the membrane. In contrast, adrenaline (10(-8)-10(-6) g/ml.) hyperpolarized the membrane and increased the input resistance.3. Noradrenaline had no effect on the amplitude of the miniature junction potentials but caused them to become more frequent.4. Adrenaline enhanced the amplitude of the miniature junction potentials and slightly increased their frequency. These effects of adrenaline were partly due to the increased input resistance of the post-synaptic membrane, and probably did not involve the presynaptic terminals.5. Both noradrenaline and adrenaline increased the amplitude and prolonged the falling phase of the excitatory junction potentials (e.j.p.s).6. Noradrenaline enhanced the amplitude and prolonged the duration of the extracellularly recorded excitatory junction current. No such effect was observed with adrenaline.7. Adrenaline but not noradrenaline enhanced the amplitudes of acetylcholine potentials evoked by iontophoretic application of acetylcholine.8. Noradrenaline and adrenaline had no effect on the compound action potentials of the nerve supplying the red muscle, recorded by the sucrose gap method.


Adrenocortical insufficiency related to oral contraceptives.


Infection of hamster spleen and thymus with herpes simplex virus in vitro and in vivo.


Susceptibility of human lymphoblasts (RPMI 7466) to viral infections in vitro.


Metabolism of lecithin and virus-induced cell fusion.


In vitro stimulation of renin production by epinephrine, norepinephrine, and cyclic AMP.


The effect of interferon on focus formation and yield of murine sarcoma virus in vitro.


Glucagon and epinephrine responsive adenyl cyclase activity of regenerating rat liver.


Reversal of streptozotocin diabetes with nicotinamide.


Rapid eye movement sleep deprivation: a central-neural change during wakefulness. Three adult cats were deprived of rapid eye movement sleep for six separate periods of up to 32 days. Animals were allowed normal amouints of sleeping time during which rapid eye movement sleep was interrupted, whenever it occurred, by human observers who continually monitored the animals and their electrocortical activity. Cortical responses evoked by pairs of acoustic clicks were recorded during wakefulness. Recovery functions derived from these data were facilitated during periods of deprivation of rapid eye movement sleep and returned to base-line values when animals were allowed normal amounts of this sleep phase. This change was noted repeatedly within, as well as between, subjects. It did not occur during control periods when non-rapid eye movement sleep was interrupted on identical schedules, nor did it occur when the cats were deprived of all sleep for 22 hours a day for 5 days.


Polyoma virus gene activity during lytic infection and in transformed animal cells. Randomly labeled RNA's from animal cells either productively infected or transformed by polyoma virus were used to measure virus DNA transcription. During lytic infection, 50 percent of polyoma virus DNA was expressed. In two different polyoma-transformed hamster cell lines, the same 20 percent of the virus genome was transcribed. About 10 percent of polyoma DNA was expressed in the one mouse transformed cell line examined.


Endocrine control of adrenal progesterone secretion in the ovariectomized rat. In the ovariectomized rat, the adrenal gland secretes progesterone. Adrenocorticotropin treatment elevates concentrations of this steroid in plasma, and inhibitors of adrenocorticotropin or progesterone biosynthesis lower the concentration. Adrenocorticotropin controls progesterone secretion by the adrenal but not by the ovary. Adrenal progesterone and its mode of control may have important influences on reproductive processes.


Partial inactivation of angiotensin II by plasmin.


Fusidic acid: inhibition of factor T2 in reticulocyte protein synthesis. The steroid antibiotic fusidic acid inhibits reticulocyte protein synthesis. This inhibition appears to be due to interference with the activity of the T(2) supernatant fraction, and strengthens the proposition that T(2) is functionally analogous to the G-factor of bacterial protein synthesis, which is also specifically inhibited by this antibiotic.


Mammary tumor virus antigen: sensitive immunoassay. A rapid, sensitive immuno-assay for mammary tumor virus antigen based on inhibition of passive hemagglutination has been developed. The method permits measurement of this antigen in mouse milk from which the fat has been removed.


Polyneuropathy in childhood.

Polyneuropathy in childhood.


"Retinal anlage" tumors. Melanotic progonoma, melanotic adamantinoma, pigmented epulis, melanotic neuroectodermal tumor of infancy, benign melanotic tumor of infancy.


Cerebral gigantism.

Cerebral gigantism.


Adrenal medullary contribution to the progressive pressor response to angiotensin in the rabbit.


Electron-spin-resonance evidence for enzymic reduction of oxygen to a free radical, the superoxide ion. 1. An electron-spin-resonance signal with g( parallel)2.08 and g( perpendicular)2.00 is observed by the rapid-freezing technique during the oxidation of substrates by molecular oxygen catalysed by xanthine oxidase at pH10. 2. The intensity of this signal is shown to depend on oxygen rather than on enzyme concentration, indicating that it is due to an oxygen free radical and not to the enzyme. 3. The same species is shown to be produced in the reaction at pH10 between hydrogen peroxide and periodate ions. Studies with this system have facilitated comparison of the properties of the oxygen radical with data in the literature on the products of pulse radiolysis of oxygenated water over a wide pH range. 4. It is concluded that the species observed is the superoxide ion, O(2) (-), and that the stability of this ion is greatly increased in alkaline solution. A mechanism explaining the alkaline stability is proposed. 5. The importance of O(2) (-) in the enzymic reaction is discussed.


Effect of an extract of UV-irradiated linolenic acid on azo dye carcinogenesis.


Evidences for a hypocalcemic factor from pituitary gland.


Sir Henry H. Dale, O.M., G.B.E., M.D., F.R.C.P., F.R.S.


Chemistry of lipid of the posthemyolytic residue or stroma of erythrocytes. XVI. Occurrence of ceramide pentasaccharide in the membrane of erythrocytes and reticulocytes of rabbit.


Water and electrolyte shifts in the presence of two vasoactive substances.


Evidence for thyrocalcitonin binding to protein in plasma.


Renal function in the European eel (Anguilla anguilla L.): effects of the caudal neurosecretory system, corpuscles of Stannius, neurohypophysial peptides and vasoactive substances.


Synthesis of natural human angiotensin I.


The inactivation of poliovirus in aerosols.


Vertical transmission of murine leukemia virus.


Vertical transmission of murine leukemia virus through successive generations.


Preliminary report on virus-like particles in a transplantable rat mammary carcinoma.


Phorbol ester tumor-promoting agents and membrane stability.


A comparative cytologic study of the cultivation of hepatomas of different growth rates.


Additional studies of interferon production by human leukemic leukocytes in vitro.


Lymphocytosis-promoting factor produced by Bordetella pertussis. Partial purification and characterization.


Rapid titration of high concentrations of SV40 virus by immunofluorescence.


Genetics and cancer.

Genetics and cancer.


Random coil conformation of polypeptide hormone precursor protein in endocrine cells.


220 MHz proton magnetic resonance spectrum of NADH.


Free radical reactions modify cellular damage.


Autofluorescence of visual receptors.

Autofluorescence of visual receptors.


Control of Marek's disease by the use of inoculated chicken embryo fibroblasts.


Increased tyrosine hydroxylase activity after drug-induced alteration of sympathetic transmission.


A combined bovine parainfluenza and adenovirus vaccine.


The measurement of urinary testosterone glucuronide by competitive protein binding and validation by a double isotope derivative assay.


Aspects of oral development in relation to litter size in rats.


An ultrastructural and histochemical study of rat atrial myocardium.


Fine structure of Leydig cells from an arrhenoblastoma of the ovary.


In vitro culture of spleen cells from a case of Gaucher's disease.


Systemic effects of intestinal revascularization.


Cleveland Clinic Fire Survivorship Study, 1929-1965.


Comparison of motor and sensory nerve conduction velocity in early uremic polyneuropathy.


Ozone and lipid peroxidation.

Ozone and lipid peroxidation.


The course and prognosis in some types of carcinomatous neuromyopathy.


Peripheral neuropathy and myopathy associated with carcinoma of the lung.


A comparative study of peripheral nerve conduction in diabetes and non-diabetic chronic occlusive peripheral vascular disease.


Enhancement by angiotensin of pressor responses to endogenous noradrenaline in the pithed rat.


Magnetic resonance studies of anaesthetics in cyto-membranes.


Sustained complete autonomic blockade.

Sustained complete autonomic blockade. 1. A 3-hr period of complete autonomic blockade was induced in anaesthetized sheep and dogs using a continuous intravenous infusion of trimetaphan camphorsulphonate and atropine. Circulatory, respiratory and metabolic parameters were studied over 24 hr or until the animal died, whichever came first.2. After termination of the blockade all animals remained hypotensive and within a few hours all died in irreversible shock which was not accompanied by any of the otherwise characteristic respiratory or metabolic consequences of circulatory failure.3. Administration of trimetaphan alone for a similar period was innocuous.4. When during complete autonomic blockade a continuous infusion of dopamine or hypertensin was additionally administered mortality rate was halved but all survivors remained hypotensive.


Blood angiotensin II levels of normal and hypertensive subjects. A specific radioimmunoassay for angiotensin II has shown that its normal concentration in arterial blood is 2.4+/-1.2 (S.D.) mmug./l00 ml.; the venous level is consistently below this value, being usually 50-75% of it. Definite rises in blood angiotensin II levels were found in some patients with hypertension, both essential and secondary to renal disease. Extremely low levels were observed in two anephric women, and in one patient with Conn's syndrome. This radioimmunoassay offers a valuable alternative to renin bioassay in evaluation of the role of the renal pressor system in clinical disorders associated with hypertension and aldosteronism.


The treatment of Wilms' tumour with special reference to actinomycin D.


Adenohypophysial glycogenolysis in relation to ACTH release induced by intrapituitary injection of a purified hypothalamic CRF.


Histochemical studies on steroid diabetes of guinea pigs with special reference to Langerhans islets of the pancreas.


Entamoeba histolytica: effect of metal ions, metal binders, therapeutics, antibiotics, and inhibitors on aldolase activity.


Cytochrome c oxidase in migrating larvae of Ascaris lumbricoides var. suum.


Communicability of enteric live adenovirus type 4 vaccine in families.


Studies on the mode of action of diphtheria toxin. VII. Toxin-stimulated hydrolysis of nicotinamide adenine dinucleotide in mammalian cell extracts. When diphtheria toxin and NAD are added to soluble fractions containing aminoacyl transfer enzymes isolated from rabbit reticulocytes or from HeLa cells, free nicotinamide is released and, simultaneously, an inactive ADP ribose derivative of transferase II is formed. The reaction is reversible, and in the presence of excess nicotinamide, toxin catalyzes the restoration of aminoacyl transfer activity in intoxicated preparations. In living cultures of HeLa cells, the internal NAD concentration is sufficiently high to account for the rapid conversion, catalyzed by a few toxin molecules located in the cell membrane, of the entire cell content of free transferase II to its inactive ADP ribose derivative. Completely inactive ammonium sulfate fractions containing soluble proteins isolated from cells that have been exposed for several hours to excess toxin, can be reactivated to full aminoacyl transfer activity by addition of nicotinamide together with diphtheria toxin. Transferase II appears to be a highly specific substrate for the toxin-stimulated splitting of NAD and thus far no other protein acceptor for the ADP ribose moiety has been found.


Hereditary epidermolysis bullosa.

Hereditary epidermolysis bullosa.


Inactivated myxovirus vaccines, atypical illness, and delayed hypersensitivity.


Some physiochemical properties of Yaba poxvirus deoxyribonucleic acid. Yaba tumor poxvirus deoxyribonucleic acid (DNA) has a density of 1.6905 in CsCl and its T(m) value in 0.015 m citrate in saline is 82.3. The guanine plus cytosine content estimated from these properties was taken to be 32.5 +/- 0.5%, a value 2 to 3% less than for DNA from other poxviruses reported to date.


Characteristics of the syrian hamster ribonucleic acid present in cells transformed by polyoma, simian virus 40, or adenovirus 12. Ribonucleic acids of known specific activities were separately prepared from hamster cells transformed by polyoma, simian virus 40 (SV40), and adenovirus 12. When increasing amounts of these labeled ribonucleic acids were added to nitrocellulose filters containing immobilized (14)C-hamster deoxyribonucleic acid (DNA), about 5% of the DNA sites were saturated in each case. An additive saturation-hybridization experiment indicated that a portion of the 5% of the hamster DNA transcribed in SV40-transformed cells was also transcribed in polyoma-transformed cells. In addition, a separate region of the hamster genome was also transcribed in polyoma-transformed cells.


Isolation of naturally occurring viruses of the murine leukemia virus group in tissue culture. A tissue culture cell system for isolation and identification of members of the murine leukemia virus group (the complement fixation for murine leukemia test) was modified to permit the isolation of naturally occurring virus from leukemic and normal mice. The important factors for increasing the sensitivity of the test were the use of National Institutes of Health (NIH) strain Webster Swiss embryo cell cultures and the selection of rat-immune sera having complement-fixing antibodies to tissue culture antigens of both the Gross and FMR subgroups. In all, 163 strains of mouse leukemia virus, from 11 inbred mouse strains, have been isolated. Representative virus isolates were shown to possess the properties of the murine leukemia virus group; i.e., they were chloroform-sensitive, noncytopathic agents which replicated in mouse embryo tissue culture and produced group-reactive, complement-fixing antigen and budding C-type particles visible by electron microscopy. These viruses could serve as helpers in the rescue of Moloney sarcoma virus genome from non-producer hamster sarcoma cells, yielding pseudotypes. All of the 19 field isolates tested were neutralized by Gross passage A antiserum but not by potent antisera to the Moloney, Rauscher, and Friend strains. Virus was recovered regularly from embryos and from the plasma and spleen of adult mice of high leukemic strains. In low leukemic mouse strains, different patterns of virus detection were observed. In C3H/He mice, virus was occasionally present in embryos and was found in 40% of adult spleens. BALB/c mice were virus-negative as fetuses or weanlings, but spleens of more than half of the mice over 6 months of age yielded virus. NIH mice have never yielded virus. In reciprocal matings between AKR and BALB/c mice, virus recovery from embryos was maternally determined. The development of tissue culture isolation procedures made possible for the first time the application of classical infectious disease methods to the study of the natural history of murine leukemia virus infection.


Multiple group-specific antigen components of avian tumor viruses detected with chicken and hamster sera. Multiple group-specific (gs) components of the avian leukosis-sarcoma viruses were detected by immunodiffusion (Ouchterlony) tests with sera from hamsters bearing tumors induced by sarcoma viruses and with sera from adult chickens immunized with avian sarcoma or leukosis viruses. Immune hamster sera detected up to four components, whereas chicken sera detected at least one. The hamster and chicken sera identified a similar antigen, as indicated by reactions of identity. Relatively few chicken sera containing neutralizing antibody to avian sarcoma or leukosis viruses reacted in immunodiffusion with the gs antigen. The gs components were released from the virion by various means of disruption, including freezing and thawing. Tests with tissues from normal chickens and from chickens with Marek's disease failed to demonstrate any reactions with hamster or chicken gs antiserum.


Origin of the thymidine kinase induced by polyoma virus in productively infected cells. Cells of the 3T3 mouse line efficiently supported the multiplication of polyoma virus, and the infectious process was accompanied by a marked increase in thymidine kinase (TK) activity. Two lines of 5-bromodeoxyuridine-resistant 3T3 cells have been isolated. As expected, these cells incorporated practically no exogenous thymidine into their deoxyribonucleic acid (DNA) and contained negligible TK activity. Like the parental 3T3 cells, TK(-) lines were susceptible to productive infection by polyoma virus, but infection did not lead to an increase in TK activity. Since kinase activity did appear after infection with another virus (vaccinia) known to contain the gene(s) for that enzyme, it is concluded that TK is not one of the gene products of polyoma virus. As induction of cellular DNA synthesis by polyoma virus occurs normally when the TK(-) cells are infected in the stationary phase, TK cannot play a role in the determination of this phenomenon.


Reversion in polyoma-transformed cells: retransformation, induced antigens and tumorigenicity. We studied the properties of two morphologically reverted cell clones isolated as chromosomal segregants from a "hybrid" clone of BHK 21/13 hamster fibroblasts, transformed with polyoma virus. Both clones were less tumorigenic than control transformed cells. They contained no detectable polyoma-specific complement-fixing antigen. Induced transplantation antigen also appeared to be lost or reduced. Both clones could be retransformed with polyoma virus, suggesting that their reversion is due to the loss of viral genes from the transformed cell.


Markers of rubella virus strains in RK13 cell culture. When tested on RK(13) cell cultures, strains of rubella virus could be differentiated by their ability to form small or large plaques. Large plaques were produced by the HPV-77 and Cendehill strains, and also by a laboratory stock strain (West Point), after only 14 passages in RK(13) culture. Five wild-type rubella viruses, isolated and passaged only a few times in African green monkey kidney tissue culture, grew well in RK(13) cell culture, but they were sensitive to agar inhibitors and, therefore, formed small plaques. On the other hand, RA27/3, an attenuated strain grown in WI-38 human fibroblast cells, developed low titers in RK(13) cells and also produced small plaques. We concluded that the morphological differences between small-plaque and large-plaque viruses depended on their sensitivity to agar inhibitors and on the pH of the medium during plaque formation.


Filamentous structures of type 2 Herpesvirus hominis infection of the chorioallantoic membrane. In view of recent recognition of the existence of two Herpesvirus hominis (HVH) types with antigenic and biological differences, an electron microscopic study was undertaken of pocks produced on the choriallantoic membrane of embryonated eggs after infection with type 1 and type 2 HVH strains. Besides the typical morphological features of herpesvirus infection noted by several investigators, it was observed that type 2 HVH also produced microtubules measuring approximately 19 nm in both nucleus and cytoplasm. Although the nature of these filamentous structures is still unclear, consideration is given in this paper to the possibility that they may represent viral structural subunits, aberrant forms or neoantigens.


New nuclear ribonucleic acid in cell cultures infected with polyoma virus.


Myxedema and ataxia.

Myxedema and ataxia.


Inherited predisposition to generalized amyloidosis. Clinical and pathological study of a family with neuropathy, nephropathy, and peptic ulcer.


Unilateral numbness of the face and the basal cisternogram.


Fauna of Catal Hüyük: evidence for early cattle domestication in Anatolia. Analysis of the remains of cattle from Catal Hüyük indicates that cattle were domesticated in Anatolia by 5800 B.C., and strongly suggests that they were probably domestic at least 500 years earlier. This is the earliest known evidence for the domestication of cattle in the Near East.


Infections and pregnancy: a review.

Infections and pregnancy: a review.


A review of the recent research on sleep and dreaming.


Pneumonia in gnotobiotic pigs produced by simultaneous inoculation of a swine adenovirus and mycoplasma hyopneumoniae.


Treatment of herpes simplex keratitis. A review.


Treatment of uveitis with methotrexate.

Treatment of uveitis with methotrexate.


Surgical management of solid tumors in childhood.


Response to radiation therapy of osseous lesions detected on radioisotope bone scans.


Angiographic manifestations of Wilms' tumor. An observation of six cases.


The perception-hallucination continuum. (A re-examination).


Evaluation of laboratory problems of glassblowers working fused quartz.


Study of typability of staphylococci in relation to their resistance to antibiotics.


Melanoameloblastoma: report of case.

Melanoameloblastoma: report of case.


Unexpected high incidence of tumours in thymectomized mice treated with anti-lymphocytic globulin and Mycobacterium leprae.


Induction of liver and lung tumours in mice by 6-aminochrysene administered during the 1st 3 days of life.


Pressor response to angiotensins I and II and renin in rats treated with carbon tetrachloride.


Solubilization and some properties of the erythrocyte receptor for adenovirus type 7 haemagglutinin.


The osmotic stability of lysosomes from adult and foetal guinea-pig liver tissue. 1. Lysosome-rich fractions were obtained from foetal liver tissues as early as 35 days uterine age. Foetal lysosomes showed the same ;structure-linked latency' and acid hydrolytic potentiality characteristic of their adult counterparts. 2. The osmotic stability of lysosome-rich fraction from foetal guinea-pig liver tissue was greater than that of the corresponding adult lysosome fractions, p-nitrophenyl-phosphatase being used as marker enzyme. 3. The observation was confirmed by using beta-glycerophosphatase and phenolphthalein beta-glucuronidase as alternative marker enzymes. p-Nitrophenyl phosphate and beta-glycerophosphate appear to act as substrates for the same enzyme. 4. By using p-nitrophenylphosphatase activity measurements it was shown that the osmotic stability of foetal lysosomal fractions decreased with increasing foetal age, but at no time achieved the degree of osmotic instability characteristic of adult lysosomal fractions. 5. The correlation of these findings with the intracellular environment of lysosomes is discussed.


Triglyceride synthesis by the small-intestinal epithelium of the pig, sheep and chicken. 1. A comparative study was made of triglyceride synthesis by the intestinal epithelium of pigs, sheep and chickens. In pig and chicken tissue both the glycerol 3-phosphate and the monoglyceride pathway of triglyceride synthesis were operative, but the former pathway predominated in sheep tissue. 2. The fatty acid specificity of the glycerol 3-phosphate pathway was studied in pig and sheep total-homogenate preparations. Maximum incorporation was obtained with myristic acid and palmitic acid under optimum conditions for each fatty acid. Lauric acid, myristic acid, oleic acid, linoleic acid and linolenic acid were inhibitory at concentrations above their optimum, but octanoic acid, decanoic acid, palmitic acid and stearic acid did not show this effect. 3. Subcellular fractionation located the glycerol 3-phosphate and monoglyceride pathways of triglyceride synthesis in the microsomes in all instances. Phosphatidate phosphohydrolase was associated with both the microsomes and the particle-free supernatant. 4. Glycerol 1-mono-oleate was incorporated into triglycerides to a greater extent than glycerol 1-mono-palmitate or glycerol 1-monostearate by microsomal preparations from pig and chicken. 5. A lipase specific for monoglycerides was detected in the particle-free supernatant of all the species examined.


Incorporation of [32P]orthophosphate into brain-slice phospholipids and their precursors. Effects of electrical stimulation. 1. The incorporation of [(32)P]phosphate into phospholipids was measured in slices cut from the pial surface of guinea-pig cerebral cortex; incorporation into the phosphorus of some water-soluble precursors of phospholipid was measured under similar conditions. 2. Slices subjected to overall electrical stimulation at a frequency of 5pulses/sec. differed from control slices in their pattern of phospholipid labelling. After 1hr. of stimulation, incorporation of [(32)P]phosphate into phosphatidylcholine, ethanolamine phospholipid and cardiolipin was respectively 54, 55 and 58% of the control value, and that into phosphatidylinositol was 186% of control. Phosphatidic acid labelling tended to increase with electrical stimulation, but the statistical significance of this change was marginal. Labelling of phosphatidylglycerol and di- and tri-phosphoinositides was not affected significantly by electrical stimulation. 3. Electrical stimulation of the tissue altered the specific radioactivities of water-soluble precursors of phospholipid. 4. The turnover rates of the phosphate groups of phospholipids were estimated approximately from the specific radioactivities of phospholipids and their precursors. Phosphatidylinositol (and its lipid-soluble precursors) showed the largest change in turnover rate in response to electrical stimulation of the tissue; the turnover rates of other lipids were also affected. Changes in the specific radioactivity of phospholipids did not correspond to changes in turnover in these experiments.


Phospholipid exchange reactions within the liver cell. 1. Isolated rat liver mitochondria do not synthesize labelled phosphatidylcholine from CDP-[(14)C]choline or any phospholipid other than phosphatidic acid from [(32)P]phosphate. The minimal labelling of phosphatidylcholine and other phosphoglycerides can be attributed to microsomal contamination. However, when mitochondria and microsomes are incubated together with [(32)P]phosphate, the phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine of the reisolated mitochondria become labelled, suggesting a transfer of phospholipids between the two fractions. 2. When liver microsomes or mitochondria containing labelled phosphatidylcholine are independently incubated with the opposite un-labelled fraction, there is a substantial and rapid exchange of the phospholipid between the two membranes. Exchange of phosphatidylinositol also occurs rapidly, whereas phosphatidylethanolamine and phosphatidic acid exchange only slowly. There is no corresponding transfer of marker enzymes. The transfer of phosphatidylcholine does not occur at 0 degrees , and there is no requirement for added substrate, ATP or Mg(2+), but the omission of a heat-labile supernatant fraction markedly decreases the exchange. 3. After intravenous injection of [(32)P]phosphate, short-period labelling experiments of the individual phospholipids of rat liver microsomes and mitochondria in vivo give no evidence for a similar exchange process. However, the incubation of isolated microsomes and mitochondria with [(32)P]phosphate also fails on reisolation of the fractions to demonstrate a precursor-product relationship between the individual phospholipids of the two membranes. 4. The intraperitoneal injection of [(32)P]phosphate results in a far greater proportion of the dose entering the liver than does intravenous administration. After intraperitoneal administration of [(32)P]phosphate the specific radioactivities of the individual phospholipids are in the order microsomes > outer mitochondrial membrane > inner mitochondrial membrane. 5. The incorporation of (32)P into cardiolipin is very slow both in vivo and in vitro. After labelling in vivo the radioactivity in the cardiolipin persists compared with that of the other phospholipids, whose specific radioactivities in the microsomes and mitochondrial fragments decay at a similar rate to that of the acid-soluble phosphate pool. 6. The possibility of phospholipid exchange processes occurring in the liver cell in vivo is discussed, and it is suggested that only a small but highly labelled part of the endoplasmic-reticulum lipoprotein pool is involved in the transfer.


Bacteremic shock due to gram-negative bacilli. Some concepts of pathogenesis and management based on recent developments.


Comparison of high-pressure oxygen and oxygen-carbon dioxide mixtures in treatment of Clostridium perfringens infection in mice.


Immunization against adenovirus infections.


Thyrocalcitonin: ultracentrifugation in gradients of sucrose. Analysis of thyrocalcitonin, by density-gradient ultracentrifugation at high speed, showed that its molecular weight (5000 to 6000) is considerably less than was heretofore recognized.


Release of coordinated behavior in crayfish by single central neurons. By stimulating and recording from the same interneuron at two separate points, we have shown that coordinated output to the postural abdominal muscles of crayfish can be produced by electrical stimulation of a single cell. Several central neurons can individually initiate one type of movement(for example, flexion),each producing a unique abdominal geometry.


Some aspects of the interaction between polyoma virus and cell DNA.


Adenoviruses: the nature of the virion and of controlling factors in productive or abortive infection and tumorigenesis.


Distinguishing features of well differentiated mucinous adenocarcinoma of the rectum and colitis cystica profunda.


Seasonal variation in adenosine triphosphate and nicotinamide adenosine dinucleotide in normal term placenta.


Enhancement of the infectivity of rhinovirus ribonucleic acid by diethylaminoethyl dextran.


Method for testing virus stocks for viral contaminants. A practical method for testing the purity of virus stocks has been developed and applied to reference stocks of enteroviruses. The method requires the use of a reference antiserum that is substantially free from heterotypic antibody. When selected dilutions of this antiserum are reacted with high concentrations of virus, virus intentionally allowed to escape neutralization is recovered and then identified. A contaminating virus present as a minor component of the population has a far greater probability of being revealed under the conditions of this "breakthrough" test than under the commonly used virus identity tests which customarily employ approximately 100 TCD(50) of virus and, therefore, only identify the major component of the virus population. The breakthrough test described has been used for tests of 104 reference stocks of all the enteroviruses that propagate in monkey kidney cells and human amnion cells. Although all the materials had been previously tested and approved by the commonly employed virus identity test, the breakthrough test in two instances revealed contaminating heterotypic enteroviruses present at a very low titer in the stocks. Without resorting to the stringent, elaborate, and expensive tests for absolute purity, such as those that are required to assure safety of vaccines, the use of the breakthrough test described provides reasonable assurance of purity for stock viruses to be employed as diagnostic reagents or for general laboratory research purposes where a multiplicity of viral agents and antisera are required.


Inactivation of poliovirus type 1 by the Kelly-Purdy ultraviolet seawater treatment unit. Three experiments were conducted to determine the effect of ultraviolet (UV) radiation on poliovirus-contaminated seawater. In two of the experiments, the effectiveness of the Kelly-Purdy UV Seawater Treatment Unit to inactivate poliovirus type 1 (T(1)) suspended in continuously flowing seawater was determined. In experiment 1, the observed survival ratio of poliovirus T(1) was 2.3 x 10(-4) (99.98% reduction) in 15.7 sec. No virus was detected (<0.2 plaque-forming unit/ml) in 20.6 seconds. The calculated half-life value was 1.29 sec. In experiment 2, the observed survival ratio of poliovirus T(1) was 5.9 x 10(-4) (99.94% reduction) in 11.7 sec. No virus was detected in 15.7 sec. The calculated half-life value was 1.37 sec. In experiment 3, a laboratory-controlled UV experiment designed to closely simulate the geometry of the continuously flowing seawater system, the observed survival ratios of poliovirus T(1) were 9.7 x 10(-3) (99.03% reduction) and 3.6 x 10(-4) (99.96% reduction) in 15 and 30 sec, respectively; the calculated half-life value was 2.38 sec. A statistically significant difference was found between the inactivation rates of poliovirus T(1) in the two test systems. This rate difference was attributed primarily to UV dosage and stirring effects. The data indicated that UV radiation effectively inactivated poliovirus T(1) in flowing seawater. These results validate the efficacy of the Kelly-Purdy UV Seawater Treatment Unit for use in commercial depuration systems.


Virucidal effect of transient electric arcs in aqueous systems. Bacterial and animal viruses were inactivated by high voltage electrical discharges in water. The sensitivity of phages to the immediate component of this effect was correlated to the sensitivity to ultraviolet radiation. Transient electrical arcs in weak electrolytes also generated chemical compounds which were virucidal against phages T3, T5, and varphiX174 but were only slightly virucidal against phages T2 and T4.


Inhibition of respiration in submitochondrial particles by N,N'-dicyclohexylcarbodiimide: the effect of sodium, potassium, and antibiotics which alter membrane permeability.


The enzymic deacylation of esterified mono- and di-saccharides. I. The isolation and purification of an esterase from wheat germ lipase.


Physical and chemical studies of Mengo virus variants. II. Chromatographic behavior and chemical composition.


Phosphonolipids. XIX. Synthesis of a naturally occurring ceramide aminoethylphosphonate and its enantiomer.


Pleomorphic adenoma, "mixed tumor," minor salivary gland of the palate. Case report.


Phospholipid metabolism in the molluscs. I. Distribution of phospholipids in the water snail Lymnaea stagnalis.


Acute renal vein hypertension: oliguria, hormone infusion, and volume expansion.


The differential effect of anesthetics on muscle relaxation.


External power and recent concepts in control of limb prostheses.


Metabolism of coumarin in man.

Metabolism of coumarin in man.


Correction concerning electron paramagnetic resonance in human bone mineral.


Induction of sexual excitement by the action of adrenocorticotrophic hormone in brain.


Antigenic and biologic differences in herpesvirus hominis.


Congenital rubella: the significance of virus persistence.


The evaluation of antiviral drugs in volunteers.


Interference in human viral infections: present status and prospects for the future.


Laboratory aids to the control of smallpox in countries where the disease is not endemic.


Multiplicity reactivation of animal viruses.


Chemodectoma of the anterior mediastinum. Report of a case of probable aortic body origin with arteriographic findings.


Granular-cell myoblastoma--an unusual rectal neoplasm. Report of a case.


Force, shortening, and work in muscular contraction: relative contributions to overall energy utilization. The amounts of energy used during muscular contraction under different loads have been compared by measurement of the total amount of disturbance of the concentration of reduced nicotinamide-adenine dinucleotide during the period of oxidative recovery. The results are in quantitative agreement with the concept that three parameters determine the energy utilization: namely the time-integral of the force development, the time-integral of the shortening process, and the mechanical work.


Cytochromes: chemical and structural aspects.


Phenotype: postnatal development.

Phenotype: postnatal development.


Chemotherapy and surgery in the treatment of gestational trophoblastic neoplasms.


Long time survivals of patients with cancer of the eye and surrounding structures treated with radiation therapy.


Removal of AMP from NADH solutions.

Removal of AMP from NADH solutions.


Application of glass fiber chromatography for separation of C19 steroids.


Studies of hypertension and carbohydrate intolerance associated with Wilms tumor.


Focal nodular hyperplasia of the liver: the solitary cirrhotic liver nodule.


A strategy for discharging long-term patients.


New antiviral antibiotics; xanthocillin X mono- and dimethylether, and methoxy-xanthocillin X dimethylether. I. Isolation and characterization. (Studies on antiviral and antitumor antibiotics. V).


Ascochlorin, a new antibiotic, found by the paper-disc agar-diffusion method. I. Isolation, biological and chemical properties of ascochlorin. (Studies on antiviral and antitumor antibiotics. I).


Effect of selected antibiotics and other inhibitors on competence development in Haemophilus influenzae.


Effects of conditioning afferent volleys on variability of monosynaptic responses of extensor motoneurons.


Effect of muscle and cutaneous afferent nerve volleys on excitability fluctuations of Ia terminals.


Electrical activity in cat olfactory cortex produced by synchronous orthodromic volleys.


Synaptic organization of cat olfactory cortex as revealed by intracellular recording.


Hyperpolarizing influence of trigeminal nucleus caudalis on primary afferent preterminals in trigeminal nucleus oralis.


Responsiveness of thalamic and cortical motor relays during arousal and various stages of sleep.


Cerebellar glioblastomas.

Cerebellar glioblastomas.


Enzyme activities in isolated glomeruli of the rat with renal hypertension.


The effect of mercuric chloride, of bilateral ureteral ligation and of bilateral nephrectomy on plasma renin substrate concentration in rats.


Plaque formation by herpes simplex viruses in GMK (established grivet monkey kidney) cells.


Neurotransmitter quantum released from sympathetic nerves in cat's skeletal muscle.


Rapid corticotropin test with 1-24 peptide.


Carbohydrate metabolism of choriocarcinoma in continuous tissue culture.


Sleep, dreaming and drugs.

Sleep, dreaming and drugs.


Controversies in the treatment of rheumatoid arthritis.


Toxic chemicals and peripheral neuropathy: clinical and epidemiological features.


Toxic chemicals and peripheral neuropathy.


Toxic chemicals and peripheral neuropathy: experimental studies.


A system of secondary prevention in a psychiatric aftercare clinic of a general hospital.


Dynamics and significance of cerebral protein metabolism.


Metabolism of adenosine 3',5'-monophosphate by epithelial cells of the toad bladder. When whole urinary bladders of the toad were incubated with adenosine 3',5'-monophosphate-(3)H (cyclic AMP-(3)H) > 95% of the radioactivity could be extracted from the tissue with trichloroacetic acid (TCA). The TCA-soluble radioactivity was separable by cation-exchange (Dowex-50) chromatography into residual cyclic AMP-(3)H, 5'-AMP-(3)H, adenosine diphosphate (ADP)-(3)H and inosine-(3)H. Thus, neither substantial tight binding of cyclic AMP to TCA-precipitable cell constituents nor any novel metabolite of cyclic AMP were found. On exposure of cyclic AMP-(3)H to a crude homogenate of the epithelial cells scraped from the mucosal face of the bladder, the principal metabolite was inosine-(3)H, whereas 5'-AMP-(3)H was either absent or present in undetectible amounts. However, when the homogenate included added 5'-AMP (2 x 10(-2) mole/liter), substantial quantities of 5'-AMP-(3)H were recovered. Metabolism of cyclic AMP-(3)H by homogenates of the epithelial cell scrapings from the bladder was strongly stimulated by alkalinization over the range in pH of 6-9. Theophylline inhibited metabolism of cyclic AMP only to a limit of 50%, the inhibition being limited to the OH(-)-stimulated component. These results suggest the possibility that a second pathway for metabolism of cyclic AMP may exist. If such is the case, its relationship, if any, to the ultimate biological effects of cyclic AMP within cells will be studied.