Use of glutamic acid to supplement fluid medium for cultivation of Bordetella pertussis. The amino acid consumption by Bordetella pertussis growing in broth containing casein hydrolysate was examined. Serine, proline, alanine, glycine, aspartate, and glutamate were rapidly consumed, in a manner which suggested that they supplied the energy requirements of the organism; exhaustion of the energy source appeared to be the main factor limiting the yield of cells. There was no correlation between the utilization of individual amino acids and the phase of growth; uptake appeared to depend only upon relative concentrations. Consumption of threonine, phenylalanine, histidine, leucine, and methionine was slight; consumption of valine and lysine was variable, and isoleucine was excreted. The addition of monosodium l-glutamate (3 mg/ml) to the broth in shaken flasks increased the cell yield by an average of 43.5%. It had no detectable adverse effect upon the agglutin-producing capacity, agglutinability in antisera versus smooth and rough growth phases, mouse-lethal toxicity, histamine-sensitizing factor potency, or intracerebral protective potency of the culture. Broth supplemented with monosodium l-glutamate has been used over a 2-year period to prepare experimental vaccines by both batch and continuous cultivation methods at controlled pH; the cell yields obtained from the supplemented broth have been up to 52% higher than those from the basal broth. The use of glutamate to replace a proportion of casein hydrolysate in the broth caused a reduction in the cell yield, an alteration in cell morphology, and reduction in the mouse-lethal toxicity, the histamine-sensitizing factor potency, and the intracerebral protective potency of the cells.


Endocrine dysfunction with lung carcinoma.


Aldolases as liver function tests. Fructose-1-phosphatase and fructose-1,6-diphosphatase.


Effects of antidepressant drugs of the imipramine type on central 5-hydroxytryptamine neurotransmission.


Platelet lipids. IV. 14C labeled acetate incorporation by isolated platelets.


Radicals formed by the reaction of electrons with amino acids in an alkaline glass.


Effect of drugs on hepatic drug metabolism in the fetus and newborn.


Effect of dopamine on the biological activity and in vitro release of ACTH and FSH.


Effect of an oral contraceptive agent on blood pressure response to renin.


Rhinovirus inhibition by 3-substituted triazinoindoles.


Growth of parainfluenza virus in perfused human embryonic lung.


Cyclic AMP mediation of bradykinin-induced stimulation of mitotic activity and DNA synthesis in thymocytes.


Potentiation of the crop-sac stimulating action of prolactin by ATP.


Potentiation by alpha-methyldopa of pressor responses to angiotensin.


Diethylaminoethyl-dextran enhancement of interferon induction by a complexed polyribonucleotide.


Effect of cyclic 3',5'-adenosine monophosphate and theophylline on lymphocyte transformation.


Effects of angiotensin on canine renal blood flow.


Effect of agar, calf embryo extract, and polyanions on production of foci of transformed cells by Rous sarcoma virus.


The formation of variants with a reversion of properties of transformed cells. IV. Loss of detectable polyoma transplantation antigen.


Histone synthesis in polyoma- and SV40-infected cells.


Wound tumor virus: purification and fractionation of the double-stranded ribonucleic acid.


Influence of inhibitors of protein synthesis on interferon formation in mice. II. Comparison of effects of glutarimide antibiotics and tenuazonic acid.


Antigens specified by herpesviruses. I. Effect of arginine deprivation on antigen synthesis.


Observations on the envelope properties of RSV(O).


Prednacinolone, a new anti-inflammatory steroid. 2. Biochemical and pharmacological properties.


A modern approach to the management of childhood asthma.


Studies on antiprotozoans. II. Synthesis and biological activity of some N-alkylamino-nitroimidazoles.


District nursing sister attached to hospital surgical department. An experiment of attaching a district nursing sister to the surgical department of a general hospital was designed to show the use of the district nursing service for the after-care of patients discharged from hospital after surgical treatment. In a 15-week period about 590 bed days were saved, and only six out of 126 patients discharged early had to be readmitted. Most of the patients and the general practitioners who replied to questionaries about the scheme were in favour of it.


Foot-and-mouth disease: a definition of the problem and views on its solution.


Malate dehydrogenase of ox kidney. Comparison of the reactivity of sulfhydryl groups and their relationship to activities of the mitochondrial and cytoplasmic enzymes.


Regulation of nitrate reductase activity in Neurospora crassa.


Pathways of hydrogen in mitochondria of Saccharomyces carlsbergensis.


Retinal function in an isolated, perfused mammalian eye.


Influence of detergents on enzymic activity of cactus mitochondria.


Modulation in nucleoside diphosphatase activity of mammotrophic cells of the rat adenohypophysis during secretion.


Serologic evidence of an etiologic role for bluetongue virus in hydranencephaly of calves.


A quantitative histochemical study of intestinal mucosa after x-irradiation.


Extranasopharyngeal juvenile angiofibroma--a case report.


Antibodies against commonly isolated enteroviruses in sera from cases of epidemic encehalitis and contacts (Lucknow).


Chemodectomas of the vagal body.

Chemodectomas of the vagal body.


Catecholamines and dibenzyline in trauma and adaptation to trauma.


Fringe medicine.

Fringe medicine.


Effect of tetrodotoxin on electrically-induced release of autonomic mediators in the rabbit sinoatrial node.


Peripheral sensorimotor neuropathy in association with a seminoma of an undescended testicle.


Peripheral nerve and root lesions developing as a result of haematoma formation during anticoagulant treatment.


Induction of cytochromes in human cells by oxygen. The mechanism of cytochrome induction in human cells was investigated. Cultured fibroblasts grown in low oxygen had markedly reduced contents of cytochromes A + A(3), B, and C + C(1) as measured by absorption spectra. Chloramphenicol but not cycloheximide or actinomycin blocked the initial increase in cytochrome oxidase activity in cells shifted from low to ambient oxygen, suggesting that initiation of enzyme induction requires mitochondrial but not cytoplasmic protein synthesis or transcription of nuclear genes. Turnover of cytochrome oxidase was demonstrated in cells in stationary phase.


Translation-level control of amylase and protein synthesis by epinephrine. The synthesis of RNA, protein, and amylase has been studied in normal and epinephrine-treated slices of rat parotid gland. Epinephrine stimulates synthesis of total cell protein and specifically of amylase, but amylase synthesis is preferentially increased. There is no change in either the specific radioactivity or pattern of labeling of total RNA. In the presence of actinomycin D, epinephrine stimulates secretion of amylase, as well as protein and amylase synthesis. RNA synthesis under these conditions is reduced to 10 per cent of control values. Slices obtained from animals treated with actinomycin D nine and a half hours prior to study show responses to epinephrine comparable to those from control animals, with RNA synthesis inhibited by 70 per cent. Since amylase synthesis is stimulated by epinephrine in the absence of RNA synthesis, control of the production of this enzyme is probably exerted at the level of translation.


SV40-specific RNA in the nucleus and polyribosomes of transformed cells. Cells transformed by the oncogenic virus SV40 are known to contain viral DNA integrated into cellular DNA and to produce virus-specific RNA. It has been shown that nuclear molecules containing virus-specific sequences are considerably longer than presumed virus-specific mRNA molecules from cytoplasmic polyribosomes. This finding suggests the possibility that cytoplasmic mRNA is derived by the specific cleavage of larger nuclear RNA.


Stimulated formation of adenosine 3',5'-cyclic phosphate in cerebral cortex: synergism between electrical activity and biogenic amines. When cortical slices are incubated with adenine-(14)C, adenine nucleotides are labeled in a small and relatively stable pool. The ATP-(14)C of this pool is readily converted to cAMP-(14)C during incubations with depolarizing agents, such as K(+), ouabain, veratridine, or batrachotoxin. During incubations with these agents, release of acetylcholine and of adenosine into the medium is enhanced. The increase in release of adenosine parallels the enhanced formation of cAMP-(14)C elicited by depolarizing agents, providing further evidence that adenosine may serve to couple electrical activity in the central nervous system with formation of cAMP. When adenosine or a depolarizing agent are incubated, together with a biogenic amine, such as histamine, serotonin, or norepinephrine, the combined effect on cAMP-(14)C formation in cortical slices is much more than additive. Extracellular levels of biogenic amines could in this manner modulate cAMP formation and biochemical responses in nervous tissue during electrical activity.


The reductive inactivation of UDPgalactose-4-epimerase from yeast and E. coli. The prosthetic groups of UDPgalactose-4-epimerase from yeast and E. coli can be reduced by incubation with D-galactose or L-arabinose provided that 5' uridylic acid (5'UMP) is also present. This type of concerted reduction can also be performed using sodium borohydride. The latter type of reduction also depends on the addition of 5'UMP. Enzymatic analysis of the released prosthetic group by means of lactic dehydrogenase and pyruvate revealed that the pyridine nucleotide of the reduced epimerase generated in the concerted reaction is dehydrogenated in the 4-position of the pyridine ring. The catalytic activity of the reduced epimerase is much lower than that of epimerase (10-15% of native epimerase). This reductive inactivation was also found in crude epimerase preparations from E. coli, again provided 5'UMP is present. Fractionated E. coli epimerase subjected to the concerted reduction show a striking fluorescent enhancement of the order of magnitude of that observed in the purified yeast epimerase. Unlike the latter, however, E. coli epimerase also becomes fluorescent upon addition of the specific substrate UDPgalactose. The physiological aspects of the phenomenon as related to induction of the galactose genes is discussed.


Proteins specified by herpes simplex virus. 3. Viruses differing in their effects on the social behavior of infected cells specify different membrane glycoproteins. We examined the electrophoretic properties of the proteins and glycoproteins in the smooth membranes and virions purified from cells infected with herpes simplex virus strains differing with respect to their effects on the interaction of cells among themselves. The data show the following: (1) The glycoproteins in virions and binding to membranes share common features but vary quantitatively and qualitatively depending on the virus strain. (2) The binding to smooth membranes is ordered and not random. (3) Differences in the glycosylation of membrane proteins in African green monkey (VERO) and human (HEp-2) cells indicate that glycosylation is at least in part determined by the host.


Protein factors involved in polypeptide chain initiation in Escherichia coli.


Interconversion of phospho- and dephospho- forms of pig heart pyruvate dehydrogenase. Pyruvate dehydrogenase from pig heart exists in active and inactive forms. Interconversion from the active (dephospho) form into the inactive (phospho) form is catalyzed by an ATP-dependent kinase. Conversely the enzyme is reactivated by a phosphatase which removes the phosphate group from the protein. By gradient centrifugation pyruvate dehydrogenase was prepared free of phosphatase but still containing the kinase. Reactivation of pyruvate dehydrogenase is stimulated by adenosine 3',5'-cyclic phosphate. There is incorporation of (32)P from gamma-(32)P-ATP into the protein fraction containing the phosphatase and this phosphorylation reaction is also stimulated by adenosine 3',5'-cyclic phosphate. The participation of this phosphate in the pyruvate dehydrogenase interconversion system suggests that, in heart muscle, pyruvate oxidation may be under hormonal control by a mechanism similar to that involved in the regulation of glycogen synthesis and breakdown.


Purification and properties of initiation factors F1 and F2.


Isolation and some properties of the amino acid polymerization factors from Escherichia coli.


Purification and properties of factor G.


Interaction of guanosine triphosphate with E. coli soluble transfer factors.


Protein biosynthesis: studies using synthetic and viral mRNAs.


Peptide chain elongation.

Peptide chain elongation.


Further studies on bacterial polypeptide elongation.


The RNA metabolism of nucleoli and mitochondria in mammalian cells.


Inactivation of aminoacyl transferase II by diphtheria toxin.


Mutants of Escherichia coli blocked in protein synthesis: mutants with an altered G factor.


Studies on transferase II using diphtheria toxin.


Adenosine diphosphoribosylation of aminoacyl transferase II by diphtheria toxin.


A proposed mechanism for ADP ribosylation of aminoacyl transferase II by diphtheria toxin.


The 5'-terminus of f2 RNA and the coat protein gene.


The formation of poliovirus proteins.

The formation of poliovirus proteins.


Translation of poliovirus RNA by an E. coli cell-free system.


Nonribosomal polypeptide synthesis: the biosynthesis of a cyclic peptide antibiotic, gramicidin S.


Enzymic adenosine diphosphoribosylation of nuclear proteins.


Biosynthesis of cyclic oligopeptide.

Biosynthesis of cyclic oligopeptide.


Synaptic organization in the uropile of the lateral eye of Limulus.


Isolation from turkeys of a cell-associated herpesvirus antigenically related to Marek's disease virus.


Association of stomatitis and paronychias due to herpes simplex.


The lipid composition of tissues in Niemann-Pick disease.


Metabolism of dibutyryl cyclic adenosine 3',5'-monophosphate during its regulation of lipolysis and glucose oxidation in isolated rat epididymal adipocytes.


Purification of rabbit liver fructose 1,6-diphosphatase by substrate elution.


Structural analysis of nucleolar precursors of ribosomal ribonucleic acid. Studies on the 5'-terminal and alkali-resistant dinucleotides of nucleolar high molecular weight ribonucleic acid.


Structural analysis of nucleolar precursors of ribosomal ribonucleic acid. Comparative hybridizations of nucleolar and ribosomal ribonucleic acid with nucleolar deoxyribonucleic acid.


Isolation and purification of a nuclear 4.4 S ribonucleic acid of the Novikoff hepatoma.


17 Beta-estradiol dehydrogenase of ovine ovaries.


Studies on the respiratory chain-linked reduced nicotinamide adenine dinucleotide dehydrogenase. XVII. Reaction sites of piericidin A and rotenone.


Microvascular effects of collagenase.

Microvascular effects of collagenase.


Biosynthesis and characteristics of brain protein and ribonucleic acid in mice subjected to neonatal infection or undernutrition.


The age change of acid mucopolysaccharides in the periodontal membrane of man.


Correlation of collagenase production to periodontal disease.


The prevention of ascending, catheter-induced infections of the urinary tract.


Ultrastructural features of the root surface of deciduous teeth in patients with hypophosphatasia.


The developmental concepts of Piaget applied to nursing assessment and interventions with atypical children.


Levels of utilization: nursing specialists in community mental health.


The role of the psychiatric nurse in the rehabilitation of the schizophrenic patient.


Undesirable patient behavior: improvement with monetary reward.


The psychiatrist-nurse team and home care in Soviet Union and Amsterdam.


Psychiatric drugs and trends. Drug abuse - the 1969 social concern. II.


Am I my brother's keeper?

Am I my brother's keeper?


The mental hospital patient's perception of the aide as a psychotherapist.


Do you read the nurses' observation notes?


Who am I? A patient--No. I am.

Who am I? A patient--No. I am.


The First Lady of psychiatric nursing retires (Dr. Esther Garrison).


Involvement--a way of nursing.

Involvement--a way of nursing.


Free associating on mental health manpower.


The long road back.

The long road back.


Programs for culturally deprived and retarded youngsters. An international commentary.


Problems in the after care management of the schizophrenic patient.


A method for the determination of dATP and dTTP in picomole amounts.


Enzymic degradation of isotopically labeled compounds. I. Degradation of 14C-labeled glycerol.


Improved ultracentrifuge cells for high-speed sedimentation equilibrium studies with interference optics.


An evaluation of film detection methods for weak beta-emitters, particularly tritium.


Enzymic degradation of isotopically labeded compounds. II. Glucose labeled with 14C and tritium.


The significant others off stage.

The significant others off stage.


The role of the nurse with the drug abuser and addict.


Clinical psychology in the mental health service of asocialist community.


Variable decomposition by environmental contaminants in air of estrogens on glass plates coated with silica gel for thin-layer chromatography.


A simplified MAK column procedure for separation of RNA subfractions.


Biology of the mycobacterioses. Biochemical study on the specificity of the hemagglutination reaction by the phosphatide of the tubercle bacillus.


Training the nurse in psychiatric group work.


Biology of the mycobacterioses. Structure of mycobacterial phosphatides.


Isolation and identification of a new pteridine, neopterinyl-3'-beta-D-glucuronic acid from Bacillus subtilis.


Behavior modification utilizing a token economy program.


Observations on the blood glucose of Lumbricus.


Calcium pyrophosphate crystal synovitis with articular chondrocalcinosis ("pseudogout" syndrome).


Myxovirus antibody increases in human connective tissue disease. Antibodies to measles and parainfluenza type 1 viruses were significantly increased in systemic lupus erythematosus and Reiter's syndrome. Of the individuals with highest titers of measles antibody, 75 percent had neurologic illness. Persistent virus infection may be a factor in the pathogenesis of these diseases.


On standards for psychiatric facilities: philosophically understandable but practically disappointing.


Melatonin synthesis: adenosine 3',5'-monophosphate and norepinephrine stimulate N-acetyltransferase. Treatment of cultured rat pineal glands with norepinephrine or dibutyryl adenosine 3',5'-monophosphate causes a six- to tenfold stimulation of N-acetyltransferase. This enzyme converts serotonin to N-acetylserotonin, the immediate precursor of melatonin. The increased synthesis of melatonin caused by norepinephrine treatment appears to be the result of stimulation of N-acetyltransferase by an adenosine 3',5'-monophosphate mechanism.


Adenyl cyclase activity in Morris hepatomas 7777, 7794A, and 9618A.


Changes in the nucleotide compositions of nucleolar 45 S RNA of azo dye-induced hepatoma.


Release of leukemia C particles from murine cell lines infected with Bittner virus-containing inocula.


Occurrence, pathological features, and propagation of gonadal teratomas in inbred mice and in rabbits.


Benzpyrene hydroxylase activity and its induction by methylcholanthrene in Morris hepatomas, in host livers, in adult livers, and in rat liver during development.


The biocleavage of alkyl glyceryl ethers in Morris hepatomas and other transplantable neoplasms.


Nephroblastomas induced in ovariectomized rats by dimethylbenzanthracene.


Cyclic nucleotides on cell growth in vitro.


Isolation of cyclic 3',5'-adenosine monophosphate on a neutral silicic acid-glass microfiber matrix.


Metabolic adaptations in rat hepatomas: altered regulation of serine dehydratase synthesis by glucose and amino acids in hepatocellular carcinomas.


Potentiation of the pressor response to angiotensin by amphetamine and ephedrine.


Intestinal microflora in a chronic carrier of Vibrio cholerae.


Production of interferon by long-term suspension cultures of leukocytes derived from patients with viral and nonviral diseases.


Temperature and relative humidity requirements of species of Penicillium isolated from yellow dent corn kernels.


A new class of ultralong-acting local anesthetics.