Histoenzymology of the developing human hippocampus.


Aseptic osseous necrosis and corticosteroid therapy.


Renal disease in juvenile rheumatoid arthritis.


Binding and properties of NAD+ in glyceraldehydephosphate dehydrogenase from lobster-tail muscle.


Function and role of NAD+ in mechanism of action of rabbit-muscle glyceraldehydephosphate dehydrogenase.


Enzymatic hydrolysis of adenosine 3'-5'-cyclic phosphorothioate.


Regulatory properties of primate sperm phosphofructokinase.


Cyclic 3',5'-nucleotide phosphodiesterase. Preparation of a partially inactive enzyme and its subsequent stimulation by snake venom.


The renin-like enzyme of rabbit uterus.

The renin-like enzyme of rabbit uterus.


Stabilization of deoxycytidylate deaminase from mammalian tissues.


Intermediates in the hydrolysis of ATP by human alkaline phosphatase.


Lysosomal localization of sphingomyelinase in rat liver.


The oxidation of neuraminyl groups: a new protein-coupling agent.


Incorporation of alanine-14C and leucine-14C into protein of Escherichia coli under the influence of insulin.


Isolation and partial characterization of the exocellular polysaccharides of Penicillium charlesii. II. The occurrence of phosphate groups in high molecular weight polysaccharides.


Purification and properties of acyl phosphatase from human erythrocytes.


Molecular size and feedback-regulation characteristics of bacterial asartate transcarbamulases.


The hydroxylamine reductase of mitochondria.


Stabilit of the glycosidic bond of S-adenosylsulfonium compounds toward acid.


Glutamine synthetase of Neurospora crassa. Inactivation by urea and protection by some substrates and allosteric effectors.


Metabolic activities of mechanically and enzymatically prepared rat liver cells.


Methionine biosynthesis in Ochromonas malhamensis.


Lymphoma and glandular fever.

Lymphoma and glandular fever.


Nasal mucosal absorption of tetracosactrin as indicated by rise in plasma fluorogenic corticosteroids. Tetracosactrin has been administered to six healthy dexamethasone-suppressed subjects by nasal insufflation. The pronounced increase of the plasma fluorogenic corticosteroids suggests that the peptide is well absorbed by this route.


Intermittent corticotrophin treatment: ventilatory tests in asthmatic subjects. Ventilatory tests (F.E.V.(1)/F.V.C.) have been studied in 16 asthmatic subjects during intermittent administration of corticotrophin, and the responses correlated with the plasma cortisol levels. The improvement in respiratory function was found to last for a longer period than the enhanced adrenocortical activity.


Comparison of depot tetracosactrin and corticotrophin gel. Depot tetracosactrin has been compared with corticotrophin gel in 12 inpatients of a rheumatic diseases unit Every patient received one subcutaneous infection each of the synthetic and the animal preparation, with serial measurements of plasma 11-hydroxycorticosteroids, 12-hourly urinary 11-hydroxycorticosteroids, and 17-oxogenic steroids. The duration of action of the depot tetracosactrin was virtually twice that of corticotrophin gel, the dose relationship being about 0.25 mg. of depot tetracosactrin to 50 units of gel. Local reaction to the injection of depot tetracosactrin was relatively frequent.


Influence of amphetamines on plasma corticosteroid and growth hormone levels in man. Plasma fluorogenic corticosteroid and immunoreactive growth hormone levels rose significantly after the intravenous administration of methylamphetamine to healthy young men at various times of the day. The rise in corticosteroids was most pronounced in the evening and was accompanied by an increase in circulating levels of immunoreactive corticotrophin. Oral dexamphetamine also resulted in significant rises in plasma corticosteroids but not in growth hormone. These hormonal changes were accompanied by evidence of mild central stimulation. Though they may be part of an associated and non-specific response, it is more likely that they represent specific effects of amphetamines on centres in the hypothalamus or midbrain controlling secretion of corticotrophin and growth hormone releasing factors.


Delay of hormonally induced diabetes by amino-glutethimide and metyrapone in partially pancreatectomized rats bearing transplantable pituitary tumors. Concomitant failure to prevent splanchnomegaly.


Independent variation of glucagon and epinephrine responsive components of hepatic adenyl cyclase as a function of age, sex and steroid hormones.


Serotonin inhibition of in vitro insulin release from golden hamster pancreas.


An acute effect of estradiol on steroid hydroxylation by the rat adrenal.


Reactions involved in the conversion of ornithine to proline in Clostridia. An enzyme system which converts ornithine to proline was partially purified from extracts of cells of Clostridium botulinum and of Clostridium PA 3670 by fractionation with ammonium sulfate and by dialysis in the presence of 0.01 m ornithine. Nicotinamide adenine dinucleotide (NAD) was the only cofactor required for maximal activity of the partially purified system. A possible intermediate in the conversion was accumulated when a high concentration of proline was used as substrate and the NAD was maintained in the oxidized state by adding lactic dehydrogenase. Small but significant amounts of this or a similar compound were trapped with either ornithine or proline as substrate when o-aminobenzaldehyde was added to reaction mixtures. The accumulation of the o-aminobenzaldehyde reaction product was NAD-dependent with both substrates. The compound accumulated from proline was identified as Delta(1)-pyrroline-5-carboxylic acid on the basis of the melting point of the 2,4-dinitrophenylhydrazone, and by paper chromatography of the reaction product formed with o-aminobenzaldehyde. Also, extracts of C. botulinum cells oxidized reduced NAD (NADH) in the presence of the product from proline or in the presence of Delta(1)-pyrroline-5-carboxylic acid, but did not do so in the presence of the other possible intermediate, Delta(1)-pyrroline-2-carboxylic acid. (14)C-Delta(1)-pyrroline-5-carboxylic acid was reduced to (14)C-proline by these extracts in the presence of NADH. These data indicate that the conversion of ornithine to proline by C. botulinum and Clostridium PA 3679 cells involves an oxidation of ornithine to glutamic-gamma-semialdehyde which undergoes ring closure to Delta(1)-pyrroline-5-carboxylic acid. The latter compound is then reduced to proline.


Phosphorylation and the reduced nicotinamide adenine dinucleotide oxidase reaction in Streptococcus agalactiae. Cell-free extracts from aerobically grown Streptococcus agalactiae cells possess a reduced nicotinamide adenine dinucleotide (NADH) oxidase which is linked to oxygen. It is inhibited by cyanide, although cytochromes evidently are not involved. Adenosine triphosphate (ATP) formation occurs during the reaction, but 66 to 75% of the total ATP is formed nonoxidatively. The remaining 25 to 35% of the ATP formation is related to the oxidation of NADH. The formation of ATP in the oxidative reaction can be prevented by excluding oxygen or adding cyanide to prevent NADH oxidation. It can also be prevented by adding methylene blue or pyruvate, which bypasses electron transport to oxygen, but does not interfere with NADH oxidation. Potential sources of ATP, such as glycolysis, the pyruvate oxidase reaction, or the oxidative pentose cycle, are not present, and the high nonoxidative ATP formation is ascribed to the adenylate kinase reaction. The reaction requires adenosine diphosphate (ADP) as a phosphate acceptor. NADH oxidation is independent of ADP. Antimycin A, amytal, and 2,4-dinitrophenol decreased, but did not prevent, oxidative formation of ATP. P:O ratios ranged from 0.15 to 0.25. All of the oxidative activity was in the soluble portion of the cell-free extracts.


Effects of some hormonal and other factors on the excretion of guanosine 3',5'-monophosphate and adenosine 3',5'-monophosphate in rat urine.


Determination of guanosine 3',5'-monophosphate in tissues and of guanyl cyclase in rat intestine.


Composition and synthesis of gangliosides in rat hepatocyte and hepatoma cell lines.


Cyclic nucleotide-dependent protein kinases. 3. Purification and properties of adenosine 3',5'-monophosphate-dependent protein kinase from bovine brain.


Herpesvirus hominis encephalitis in Michigan. Report of thirteen cases, including six treated with idoxuridine.


Idoxuridine in herpesvirus encephalitis.


Cytomegalovirus infection in pregnancy.

Cytomegalovirus infection in pregnancy.


Thorium dioxide angiography causing bile duct carcinoma; a case report with radiological studies.


Growth of herpes simplex virus in adult human skin organ cultures.


Mediation of the mitogenic action of growth hormone by adenosine 3'5'-monophosphate (cyclic AMP).


Viability of foot-and-mouth disease virus in oil emulsions.


Effect of antithymocyte serum on herpesvirus hominis (type 1) infection in adult mice.


Yaba tumor virus. II. Studies on inactivation and reactivation.


Evidence for the susceptibility of primary fetal mouse cells in culture to coxsackievirus A13.


Inducers of interferon and host resistance. VII. Antiviral efficacy of double-stranded RNA of natural origin.


Induction of the interferon mechanism by single-stranded RNA: potentiation by polybasic substances.


Growth of ultraviolet-damaged herpesvirus in xeroderma pigemntosum.


Potassium ions asymmetrically activate erythrocyte membrane phosphatase. In Reconstituted ghosts of human red cells, the cell membrane phosphatase is activated by external, but not by internal, potassium. This finding is consistent with the possible participation of the membrane system responsible for cation transport.


Simian virus 40 in polio vaccine: follow-up of newborn recipients. Soon after birth, when susceptibility to carcinogens should be enhanced, a group of children received oral polio vaccine which was later found to contain significant amounts of simian virus 40. Eight years after the incident, no cancer deaths have been observed among the vaccinated children, but continued surveillance is needed before concluding that simian virus 40 is innocuous to man.


Studies on ACTH-binding antibodies: characterization of immunological specificities.


Necrotizing infectious enteritis in piglets, caused by Clostridium perfringens type C. 3. Pathological changes.


Necrotizing infectious enteritis in piglets, caused by Clostridium perfringens type C. IV. Bacteriological diagnosis.


Individual differences in the frequency of hippocampal theta activity in relation to the nonspecific (constitutional) excitability level in rats.


The relation between the duration of quiet and paradoxical sleep in infants.


Congestive heart failure: biochemical and physiologic observations.


Purification of complement-fixing antigens of Rickettsia orientalis by ether extraction.


Varicella-Zoster encephalomyelitis. A morphologic and virologic study.


Effects of cold injury on six enzymes in rat brain.


Experimental herpes simplex uveitis. The isolation of herpesvirus in an iris organ culture system.


A new resin system restorative designed for universal anterior and posterior applications: report of a clinical study.


Levels of glutathione in schizophrenic patients. (A biochemical-clinical study).


Bile duct carcinoma in a goat, with metastasis in the lungs.


Plasma insulin: fluctuations in the "big" insulin component in man after glucose and other stimuli. Plasma was filtered on G50 Sephadex, and two components of circulating insulin, "little" insulin and "big" insulin, were measured by immunoassay. The former component is indistinguishable from insulin, whereas the latter more closely resembles proinsulin and the other insulin-like substances isolated from the pancreas. In thin normal subjects, the fraction of plasma insulin that was big insulin (per cent "big"). 15-30 min after oral glucose, was less than 5%; per cent big rose 2- to 8-fold over the next hour and by 90-120 min represented 5-29% of the plasma insulin. In young thin subjects with idiopathic glucose intolerance associated with normal concentrations of plasma insulin, an identical pattern of big insulin was observed. In thin subjects in whom elevations of per cent big at 90-120 min during the standard test were only modest, starvation for 48 hr before the glucose administration resulted in a more pronounced rise in the per cent big insulin. Early after glucose administration to obese and acromegalic subjects, the per cent big was higher than in thin subjects. The magnitude of the elevation was roughly correlated with elevations in the fasting plasma insulin concentration. By 90-120 min, the per cent big in obese and acromegalic patients was the same range as in the thin subjects. Intravenously administered arginine and tolbutamide in a small number of subjects yielded a response that was similar to oral glucose; the per cent big was low early after stimulation and increased with time. In two patients with islet cell carcinomas, the per cent big was in the same range as in normal subjects.


Decreased myocardial adenyl cyclase activity in hypothyroidism. It has been suggested that hypothyroidism may alter the responsiveness of the heart to sympathetic stimulation. To define more precisely the interrelationship between hypothyroidism and catecholamine responsiveness we: (a) studied the effects of norepinephrine and fluoride on the activation of adenyl cyclase in the particulate fraction of heart homogenates from euthyroid and hypothyroid cats; and (b) assessed the contractile response of isolated right ventricular papillary muscles from the same cats to increasing concentrations of norepinephrine. It was found that maximal accumulation of cyclic 3',5'-adenosine monophosphate (3',5'-AMP) was significantly lower at peak norepinephrine concentrations in the hypothyroid (284 +/-5 pmoles) than in the euthyroid group (326 +/-10 pmoles) (P < 0.02). However, the K(m) for norepinephrine was similar in both groups (1-2 x 10(-5) moles/liter), and there was no apparent change in the threshold concentration. Fluoride-mediated increases in Cyclic 3',5'-AMP accumulation were also significantly lower in the hypothyroid (585 +/-25 pmoles) as compared to the euthyroid group (790 +/-20 pmoles) (P < 0.02). In contrast, norepinephrine produced a similar augmentation of contractility in isolated papillary muscles from the hypothyroid and euthyroid cats. It thus appears that although the hypothyroid state is associated with a decrease in the total amount of myocardial adenyl cyclase per milligram of tissue capable of being activated by norepinephrine or fluoride, there is no change in the sensitivity of the enzyme to norepinephrine stimulation. Moreover, the finding that the inotropic response to norepinephrine is unaltered in hypothyroidism is compatible with the hypothesis that only a fraction of the total intracellular cyclic 3',5'-AMP produced by norepinephrine activation of adenyl cyclase is required to elicit the inotropic response.


Studies on the carbohydrate metabolism of third-stage Haemonchus contortus larvae.


Immunologic tolerance and adoptively transferred immunity in polyoma virus tumor repression.


Sleep: physiologic aspects.

Sleep: physiologic aspects.


Pulmonary infection due to rhinovirus type 13.


The glioma-polyposis syndrome.

The glioma-polyposis syndrome.


Nature of renal phosphate leak.

Nature of renal phosphate leak.


Genetic resistance to leukosis caused by the JM virus in the fowl.


Fluoride toxicity in the chick.

Fluoride toxicity in the chick.


On the conformations of hallucinogenic molecules and their correlation. There are only a few possible conformations of D-lysergic acid diethylamide and hallucinogenic derivatives of tryptamine and phenylethylamine. Of these possible conformations there is a high structural correlation among the probable conformations of active hallucinogenic molecules and between these conformations and the known conformations of several central nervous system transmitter molecules.


A nondefective (competent) adenovirus-SV40 hybrid isolated from the AD.2-SV40 hybrid population. A new nondefective hybrid virus has been plaque-isolated from the Ad.2-SV40 hybrid population. This virus replicates efficiently with one-hit kinetics in both human embryonic kidney and African green monkey kidney cells, induces an SV40 specific antigen which is detectable by immunofluorescence and complement-fixation using sera from SV40 tumor-bearing hamsters, and produces SV40-specific RNA detectable by DNA-RNA hybridization. The SV40-specific antigen induced by this virus is heat-stable, sensitive to inhibitors of DNA synthesis, serologically different from SV40 T and viral antigens, and is an unrecognized SV40 antigen.


Biochemical evidence for induction by polyoma virus of replication of the chromosomes of mouse kidney cells. It was shown earlier that in cultures of contact-inhibited mouse kidney cells infection with polyoma virus induces replication of the chromosomal DNA. In this paper we present evidence, based on analyses of isolated chromatin, that, parallel with and as a consequence of the virus-induced synthesis of cellular DNA, the chromosomal proteins are synthesized in the proportions characteristic of normal chromosome replication. These results are compatible with the hypothesis that polyoma virus activates (or derepresses) a regulatory system of the host cell which controls initiation of chromosome replication.


Gangliosides in DNA virus-transformed and spontaneously transformed tumorigenic mouse cell lines. In mouse cell lines transformed by SV40 virus, a decrease was observed in the higher ganglioside homologues disialo-ceramidetetrahexoside and monosialo-ceramidetetrahexoside. Such change was observed only in cells which carry the virus genome, and it correlated with increased saturation density in tissue culture and with rejection in immunologically competent syngeneic host. This indicates that the change is one of the virus-regulated functions, and it is postulated that it relates to the rejection of the virus-transformed cells.


An assessment of various methods of treatment of idiopathic orthostatic hypotension.


Interaction of the carbohydrate-binding protein concanavalin A with normal and transformed cells. It has been shown that the carbohydrate-binding protein concanavalin A (ConA) can agglutinate leukemic cells and cells transformed by polyoma virus, simian virus 40, chemical carcinogens, and X-irradiation. This protein did not agglutinate normal cells under the same conditions. The agglutination was reversed by competition with alpha-methyl-D-glucopyranoside (alpha-MG), a carbohydrate that strongly binds to ConA, but not by the carbohydrates alpha-methyl-L-fucopyranoside or N-acetylglucosamine, with no binding or weak binding to ConA. Destruction of the alpha-MG binding sites of the native protein by removal of bivalent metal ions abolished the agglutination produced by the native protein. The treatment of cells with trypsin resulted in the agglutination of normal cells by ConA and a decrease of agglutinability of transformed cells. When nonagglutinating untransformed 3T3 cells were infected with simian virus 40 and normal rat cells were infected with polyoma virus, the infected cells became agglutinable several days after virus infection. The percentage of cells agglutinated, about 50 per cent, was much higher than the percentage of cells hereditarily transformed. The results indicate that the surface membrane of transformed cells contains sites that interact with the alpha-MG binding sites of ConA, that such sites can be found on the surface membrane of normal cells after treatment with trypsin, and that the change in the surface structure from normal to transformed occurs in cells that are abortively transformed.


Granular cell "myoblastoma" of the breast.


Carcinoma of the body of the uterus in Jamaica.


The treatment of (intestinal amoebiasis) with a single dose of humatin.


Action of polymixin and compound 48-80 on calcification in vitro.


Cyclic AMP and enzyme secretion by the isolated rabbit pancreas.


Schwann cell "necrosis" in unmyelinated nerve fibres of patients with polyneuropathy.


Fine structure and enzyme histochemistry of developing duodenal epithelium of the chicken.


Relation between chromosomal changes and succinodehydrogenase activity in the Detroit-6 (VA) cell line.


Titanium in mandibular replacement.

Titanium in mandibular replacement.


Comparative histochemical studies on the activity of some enzymes in chick embryo chorio-allantoic membranes infected with vaccinia smallpox viruses.


Correlates of home dream recall: reported home sleep characteristics and home dream recall.


Some effects of pentobarbital and strychnine on transmission through the ventrobasal complex of the cat thalamus.


Comparative effects of reserpine on the sleep cycle of man and cat.


Effect of false transmitters on norepinephrine synthesis.


A brain perfusion technique adapted for the study of drugs which may affect the peripheral circulation through a central action.


Mechanism of vascular resistance changes produced in skin and muscle by halothane.


Simultaneous assay of angiotensin I and II and determination of converting enzyme activity.


Angiotensin blocking actions of guancydine.


Sleep and instinctive behavior.

Sleep and instinctive behavior.


Electrophysiological investigations on the pathways from cerebral cortex to the anterior lobe of cerebellum.


An electrical investigation of the efferent pathways from the vestibular nuclei.


Dorsal root potentials in the lumbar cord evoked from the vestibular system.


Vestibular control of transmission in primary afferents to the lumbar spinal cord.


Primary afferent depolarization in the lumbar cord evoked from the fastigial nucleus.


Further studies of the viruses of the chimpanzee. II. Viral infection in recently imported chimpanzees captured in Africa.


Further studies of the viruses of the chimpanzee I. Viral infection of colony-maintained chimpanzees.


Effect of hormones on muscle action potentials, contractions and temperature in rats.


Axoplasmic transport in the crayfish nerve cord. Axoplasmic proteins in the crayfish nerve cord were labeled by the incorporation of high specific activity (3)H-leucine that was injected into one of the abdominal ganglia. The labeled proteins moved caudad as a sharply defined peak at 1.1 mm/day. The level of radioactivity in the cord decreased slowly as the peak approached the tail. From the sharpness of the peak and the low decrement of label with distance it is deduced that the axoplasm is probably a gel, and some of it is not consumed as it is transported along the axon but reaches the terminal and, perhaps, the synaptic regions.


RNA synthesis in cells infected with herpes simplex virus. II. Evidence that a class of viral mRNA is derived from a high molecular weight precursor synthesized in the nucleus. Viral RNA extracted from the cytoplasmic polyribosomes of human epidermoid carcinoma no. 2 cells infected with herpes simplex virus (mRNA) had a sedimentation coefficient between 10S and 20S while that from nuclei of infected cells varied in size from 10S to >80S. Estimates of the maximum molecular weight of viral RNA from its sedimentation coefficients suggest that at least 10 per cent of the viral genome is transcribed as a single molecule. The ratio of RNA of different sizes found in the nuclei of cells pulse labeled for 12 minutes was approximately the same as those found in cells labeled for longer intervals implying that either some classes of viral mRNA were made as small molecules or that the large viral RNA molecules were cleaved soon after synthesis. Cytoplasmic mRNA competed to a level of at least 80 per cent in viral DNA-RNA hybridization tests with >50S RNA extracted from nuclei of infected cells. This is consistent with the hypothesis that viral mRNA is produced by cleavage of a large precursor RNA molecule.


The replication of the ring-shaped DNA of polyoma virus. I. Identification of the replicative intermediate. The replication of the ring-shaped polyoma DNA has been investigated. The replicative intermediate has been identified and characterized; it consists of molecules containing both circular and nicked strands, not longer than the strands of the DNA found in viral particles. The findings exclude certain proposed methods of replication of ring-shaped DNA and are compatible with Cairns' model.


Stable free-radical forms of plasma proteins or simpler related structures which induce brain excitatory effects. Stable free radicals have been prepared from purified plasma proteins, pituitary peptides, and simpler related structures like 5-OH tryptophan and melatonin by oxidation with the free-radical nitrosyl disulfonate in alkaline solution under controlled conditions. The presence of tyrosine or trytophan amino acid residues in the protein was found essential for free-radical formation. These red-colored, stable free radicals showed electron spin resonance spectra in aqueous solutions at room temperature and maintained this characteristic for weeks when stored at 5 degrees C. Illumination, by visible light, of the free-radical proteins and peptides separated from excess nitrosyl disulfonate by salt fractionation or chromatography enhanced the free-radical concentration in the light. The increased signal decayed in the dark. Intravenous administration of the free-radical proteins or peptides into rabbits equipped with chronic cranial electrodes and sedated with a small dose of pentobarbital caused a sudden EEG arousal accompanied by behavioral changes indicative of brain excitation. Illumination of the free-radical compounds prior to administration enhanced the effects. Untreated control proteins or peptides had no effects. The observations are interpreted to suggest the involvement of free-radical structures in the transfer of energy in nervous tissue.


Comparative trial with highly purified natural ACTH, synthetic ACTH 1-24 peptide and synthetic ACTH 1-39 peptide.


The effect of pyruvic acid on the growth of Bordetella pertussis.


Nervous system complications in bacterial endocarditis.


The effect of oral framycetin upon colon flora and upon abscess formation about colon wounds in dogs.


Biochemical changes in the musculus complexus of the chick (Gallus domesticus).


The lipid composition of whole plasma of Hampshire sheep, Ovis aries.


Intracellular distribution of enzymes catalyzing succinate production from glucose in Rangia mantle.


Some interesting skin tumours met with in the skin department General Hospital Madras between 1964 and 1968.


Determination of prostaglandins by gas-liquid chromatography.


Single-stage clean-up of animal tissue extracts for organochlorine residue analysis.


Analytical and preparative column chromatography of neutral lipids with continuous monitoring of the eluate by a flame ionization detector.


Detection of chlorpromazine and thioridazine metabolites in human erythrocytes.


A simple thin-layer chromatography method for separating the ergotoxine alkaloids.


The separation of neomycin sulphate, polymyxin B sulphate and zinc bacitracin.


Electron microscopy of equine infectious anemia virus. Equine infectious anemia (EIA) virus was observed in thin sections of infected cultured horse leukocytes by electron microscopy. The virus particles had a spherical shape and were between 80 and 120 nm in diameter. Most of them contained an electron-dense nucleoid 40 to 60 nm in diameter. They were observed to form by a process of budding from the plasma membrane and appeared to have thin surface projections. The particles described were not detected in uninfected cultured cells, and their appearance could be prevented by adding EIA immune serum to the inoculum. The implications of these findings in the classification of EIA virus are discussed.


Ultrastructure of an intermediate Sertoli-Leydig cell tumor. A histogenetic misnomer.


The dependency of intrarenal distribution of single nephron filtration rates on dietary salt intake (micropuncture studies).


Reactions to pathogenic viruses.

Reactions to pathogenic viruses.


The epidemiology of viruses: antigenicity and evolution.


The general practitioner and the virus laboratory.


The grading of commercially bred laboratory animals.


Six years experience with herpes simplex virus in a children's home.


Application of test of adrenocortical sensitivity to bioassay of ACTH and to assessment of possible altered adrenocortical sensitivity.


Brain monoamines and stimulation or inhibition of ACTH release.


Further studies on the mechanism of the musculotropic activity of angiotensin and tyramine on the isolated guinea-pig seminal vesicle.


An experimental study of current therapeutic approaches to endotoxin shock.


Investigations on metabolites of vitamin D in rat bile. Separation and partial identification of a major metabolite. 1. Young rats with cannulated bile ducts were given 0.34mg. of [1alpha-(3)H]cholecalciferol or 0.54mg. of [(14)C]ergocalciferol by intravenous infusion. Of the radioactivity in the dose of [1alpha-(3)H]cholecalciferol 31% was recovered in bile within 24hr. 2. The metabolites in bile were separated by gradient-elution column chromatography on silicic acid into five components, all more polar than cholecalciferol or 25-hydroxycholecalciferol. [(14)C]Ergocalciferol gave a similar pattern of metabolites in bile. 3. The three most polar metabolites were shown to be ionic. The major component has been identified as a glucuronide conjugate, which was not identical with synthetic cholecalciferyl glucuronide.


Calcium ion-induced uptakes and transormations of substrates in liver mitochondria. Substrate-depleted rat liver mitochondria will reaccumulate malate, succinate, oxoglutarate, beta-hydroxybutyrate and glutamate if provided with an energy source and Ca(2+) (or Ca(2+) and Mn(2+)). The energy requirement for ion uptake by fresh mitochondria causes a transient oxidation of their NADH and presumably this leads to an increased oxaloacetate concentration. A consequence is the promotion of formation of citrate, which tends to remain in the particles, provided the pH is above 7. Analyses made of systems blocked with fluorocitrate show that citrate accumulates when Ca(2+) is added with the following substrates; (a) pyruvate in the presence of ATP or malate, (b) palmitoyl-l(-)-carnitine in presence of malate and (c) oxoglutarate. Lowering the pH, even to 6.8, causes the citrate to emerge. This could be the basis of a cellular control mechanism. The generation of citrate in response to Ca(2+) can explain the stoichiometry of one proton ejected per Ca(2+) ion taken up. The new carboxyl group formed from acetyl-CoA when it condenses with oxaloacetate provides an internal anionic charge and a proton to emerge when Ca(2+) enters.


Nicotinate, quinolinate and nicotinamide as precursors in the biosynthesis of nicotinamide-adenine dinucleotide in barley. 1. The relative efficiencies of nicotinate, quinolinate and nicotinamide as precursors of NAD(+) were measured in the first leaf of barley seedlings. 2. In small amounts, both [(14)C]nicotinate and [(14)C]quinolinate were quickly and efficiently incorporated into NAD(+) and some evidence is presented suggesting that NAD(+) is formed from each via nicotinic acid mononucleotide and deamido-NAD. 3. [(14)C]Nicotinamide served equally well as a precursor of NAD(+) and although significant amounts of [(14)C]NMN were detected, most of the [(14)C]NAD(+) was derived from nicotinate intermediates formed by deamination of [(14)C]nicotinamide. 4. Radioactive NMN was also a product of the metabolism of [(14)C]nicotinate and [(14)C]quinolinate but most probably it arose from the breakdown of [(14)C]NAD(+). 5. In barley leaves where the concentration of NAD(+) is markedly increased by infection with Erysiphe graminis, the pathways of NAD(+) biosynthesis did not appear to be altered after infection. A comparison of the rates of [(14)C]NAD(+) formation in infected and non-infected leaves indicated that the increase in NAD(+) content was not due to an increased rate of synthesis.


Temperature and the regulation of enzyme activity in poikilotherms. Fructose diphosphatase from migrating salmon. 1. The calculated energy charge of the liver cell from migrating salmon is very low (0.464), in keeping with the extended starvation and high rates of muscular and biosynthetic activity of these organisms. 2. Affinity of fructose 1,6-diphosphatase for substrate increases with a decrease in temperature. 3. Arrhenius plots of the saturation kinetics are complex and suggest an interconversion of one or more forms of the enzyme; this interconversion is dependent on the identity of the cofactor. 4. Affinity of salmon fructose 1,6-diphosphatase for its allosteric inhibitor (AMP) is lower than in other fructose 1,6-diphosphatases and this enzyme-AMP interaction is largely insensitive to temperature. The functional significance of diminished AMP-sensitivity is that it allows normal or high fructose 1,6-diphosphatase activity during a low energy charge. 5. These findings suggest mechanisms for the maintenance of high rates of gluconeogenesis in salmon during spawning migration.


The configuration of 2,6-diamino-3-hydroxypimelic acid in microbial cell walls. beta-Hydroxydiaminopimelic acid, together with some diaminopimelic acid, occurs in the cell-wall mucopeptide of certain Actinomycetales. These components were converted into their di-DNP derivatives and separated by chromatography. Hence the relative proportions present in the cell walls of a number of species were measured. The problem of acid-induced inversion of configuration was studied. Of the diaminohydroxypimelic acids isomer B (see Scheme 2; amino groups meso, hydroxy group threo to its neighbouring amino group) always predominated but a small proportion of isomer D (amino groups l, hydroxy group erythro) also occurred. The configuration of the diaminohydroxypimelic acids was determined by periodate oxidation to glutamic gamma-semialdehyde, which underwent spontaneous ring-closure. Reduction with sodium borohydride produced optically active proline, the configuration of which was determined by direct measurement of the optical rotation of DNP-proline. Un-cross-linked diaminohydroxypimelic acid in the cell wall was oxidized with periodate in the presence of ammonia. Since the remaining amino group was bound in peptide linkage, ring-closure was prevented and borohydride reduction of the aldehyde-ammonia presumed to be present resulted in the formation of ornithine. The quantity of ornithine was used as a measure of the degree of cross-linking.


Separation of the bovine colostrum M-1 glycoprotein into two components. 1. Two glycoproteins were isolated from the M-1 acid glycoprotein fraction of bovine colostrum. 2. The lighter glycoprotein had a molecular weight of 7200, contained about 28.4% of carbohydrate, and had an absorption maximum at 275nm. The heavier glycoprotein had a molecular weight of 12000, contained 39.0% of carbohydrate, and had no absorption maxima in the 240-300nm. range of the spectrum. 3. The carbohydrate moiety of both glycoproteins was removable from the polypeptide moiety under the conditions of the beta-elimination reaction. 4. Periodate oxidation experiments showed that sialic acid was linked to galactose in both proteins.


Ribosome formation in rat liver: evidence for post-transcriptional control.


Inhibitor-contaminated NADH: its influence on dehydrogenases and dehydrogenase-coupled reactions.


Reversal of N-methylisatin-beta-4',4'-dibutylthiosemicarbazone inhibition of poliovirus RNA synthesis by a photoproduct of actinomycin D.


Hyperlactacidemia as a factor of thrombocyte adhesiveness.


Influence of adrenal cortex on thyrocalcitonin content of the rat thyroid.


The cytochemistry of some enzyme activities in Stellaria media embryos.


The effects of protein synthesis inhibitors on angiotensin stimulated colonic fluid transfer.


The effects of hypercarbia on synaptic transmission in the cuneate nucleus.


The preferential release of newly synthesized transmitter by a sympathetic ganglion. 1. The acetylcholine (ACh) store of cat's superior cervical ganglia was replaced with radioactive ACh by perfusion, during stimulation, with [(3)H]choline-Locke solution. Perfusion was continued with Locke containing unlabelled choline (Ch) (in physiological concentration) and the release of labelled and unlabelled ACh was measured.2. Electrical stimulation of the preganglionic sympathetic nerve (20/sec or 5/sec), or stimulation by perfusing with raised K, released ACh that had a lower specific radioactivity than ganglionic ACh. The proportion of released ACh that was labelled was slightly higher when stimulation was at lower frequency or by K.3. Preganglionic nerve stimulation released, in the first few minutes, ACh that had a specific activity 70-80% of ganglionic ACh, but after 5 min the proportion of label in the released ACh fell to 35-45% of that in the ganglion.4. It is concluded that newly synthesized ACh is released before equilibration with preformed stores, and the significance of this to the mechanism of transmitter release is discussed.


Studies of diarrhoeal diseases in Iran. I. Occurrence of bacterial infection in pre-school children on the central plateau of Iran.


Corticotrophin release induced by injection of formalin in rats with hemisection of the spinal cord.


Pituitary-thyroid function of the rat after suppression of ACTH release by dexamethasone implants in the median eminence.


Converting enzyme activity in experimental cirrhosis of the rat.


Studies on the intestinal absorption of labelled fat-soluble vitamins (A, D, E, and K) via the thoracic-duct lymph in the absence of bile in man.


The pterygopalatine fossa and canal.

The pterygopalatine fossa and canal.


The disappearance of a large intracranial deposit of pantopaque twenty years after myelography.


Clinical experience with a new type of contrast medium in carotid arteriography.


Defective DNA animal viruses.

Defective DNA animal viruses.


Delayed hypersensitivity in mature pulmonary pertussis.


Tumors arising from pericytes. Ultrastructure and organ culture of a case.


Biosynthesis of glycosaminoglycans (mucopolysaccharides) in leukemic myeloid cells.


Nature of the increase in liver microsomal glucose-6-phosphatase activity during the early stages of alloxan-induced diabetes.


Fish liver tryptophan pyrrolase: the apparent absence of both hormonal regulation and substrate induction.


Isochorismic acid: a new intermediate in the biosynthesis of 2,3-dihydroxybenzoic acid.


Induction of glutamine synthetase by cortisol.


The enhancement of phosphoryl transfer by adenosine 3',5'-monophosphate in the presence of a membranous fraction from canine kidney.


UDP-glucose:glycogen alpha-4-glucosyltransferase I kinase activity of purified muscle protein kinase. Cyclic nucleotide specificity.


Electrical transmission between the cells of vertebrate cardiac muscle.


Neuromusclar transmission in the heart of the lobster Homarus americanus.


The lipid composition of mitochondrial and microsomal fractions of human myocardial homogenates.


Localization and mode of action of cardioexcitatory agents in molluscan hearts.


Excitation and transmission in cardiac tissues.


Administration by nasal spray of an 18 amino acid synthetic polypeptide with corticotropic action.


Association of viral ribonucleoprotein with polysomes.


Integrative activity in the nervous system: closed circuits and interneurons.


Binding of spermatozoa with surface active spin labels.


The pharmacology of spinal postsynaptic inhibition.


The proteins of the parainfluenza virus SV5. 1. Separation of virion polypeptides by polyacrylamide gel electrophoresis.


Induction of transplantation resistance to Rous sarcoma isograft by avian leukosis virus.


Recent studies on the mode of action of scorpion neurotoxins. A review.


Cell interaction with a synthetic polynucleotide and interferon production in vitro.


Familial hyperlipoproteinemia type 3.

Familial hyperlipoproteinemia type 3.


Focal epithelial hyperplasia in a Mexican Indian.


Multiple glomus tumors with a family history.


Acquired hemangiomata.

Acquired hemangiomata.


Solid hidradenoma.

Solid hidradenoma.


Dream time (REM) and psychotherapy. Correlates of REM time with a patients behavior in psychotherapy.


The pathology and treatment of haematoma of the nasal septum.


Plaque assay of poliovirus in plastic chambers.


Soluble reduced nicotinamide adenine dinucleotide oxidase from Bacillus cereus T spores and vegetative cells. I. Purification.


Soluble reduced nicotinamide adenine dinucleotide oxidase from Bacillus cereus T spores and vegetative cells. II. Properties.


The importance of implantation site in cerebral carcinogenesis in rats.


Isolation and chemical characterization of a cell-surface sialoglycopeptide fraction from novikoff ascites cells.


Effect of hepatocarcinogens on hepatocyte DNA synthesis and cortisone induction of tryptophan oxygenase.


Response of bone marrow to MC29 avian leukosis virus in vitro.


The ultrastructure of canine cutaneous papilloma.


Amino acid transport in rat liver and Morris hepatomas: effect of protein diet and hormones on the uptake of alpha-aminoisobutyric acid-14C.


Mechanism of the reduced production of aldosterone in response to loading with physiological saline solution in the rat.


Ruby laser damage and pigmentation of the iris.


Phenotypically variant adrenal tumor cell cultures with biochemical lesions in the ACTH-stimulated steroidogenic pathway.


Density dependent inhibition of cell growth in cultures of primary and established lines of cells.


Variability in the characteristics of pocks produced on the chick chorioallantois by white pock mutants of cowpox and other poxviruses.


Haemorrhagic smallpox.

Haemorrhagic smallpox.


Conjunctivitis and subclinical infection in smallpox.


A comparative study of methods for the diagnosis of respiratory virus infections in childhood.


Airborne excretion of foot-and-mouth disease virus.


Tumours of connective tissues in cattle, sheep and pigs.


Aortic body tumours in a boxer dog with a review of the literature.


Studies upon the mechanism of reserpine-induced arrest of egg transport in the mouse oviduct. I. The effect of hormone replacement.


Serodifferentiation of poliovirus strains. II. Modified Wecker test on type 3 poliovirus strains.


Antigenic characterization of poliovirus strains by neutralization kinetic studies and the modified Wecker technique.


Variants of Japanese encephalitis virus cytopathic for L mouse fibroblasts and lass human epithelial cells.


Selective determination of hemolytic activity of alpha or theta toxin of Clostridium perfringens.


Heat-labile toxin of Bordetella pertussis purified by preparative acrylamide gel electrophoresis.


Synthesis of cyclic AMP in Dictyostelium discoideum and Polysphondylium pallidum.


Phosphatase release of inorganic phosphorus in Lake Kinneret.


The development of a vaccine for bovine herpes mammillitis.


Pathogenesis of hypertension in rabbits with coarctation of the abdominal aorta.


Studies on the pathogenesis and mechanism of hematologic diversification by re-isolation of the myeloid leukemia virus (Graffi).


Overdose of tricyclic anti-depressants and deductions concerning their cerebral action.


On the mechanism of sensitivity to leucine in the case of "leucine sensitive hypoglycemia". (I).


Effect of intravenous glucagon on circulating levels of growth hormone and 17-hydroxycorticosteroids.


The role of cyclic adenosine-3',5'-monophosphate (AMP) in gastrointestinal secretion.


Anomalous response to metyrapone in Cushing's syndrome due to adrenocortical adenoma.


Anabolic and androgenic effect of testosterone in sexually immature boys and its dependency on growth hormone.


Effectiveness of long-term human growth hormone therapy for short stature in children with growth hormone deficiency.


Polydeoxyribouridylic acid and its complexes with polyribo- and deoxyriboadenylic acids.


DNA of herpes simplex virus is not a substrate for methylation in vivo.


C-21 steroid metabolism and conjugation in the human premature neonate. I. Urinary excretion and the response to ACTH.


Crystalline ternary complexes of lactate dehydrogenase.


Interaction of cassaine (an alkaloid showing digitalis-like actions) with drugs stimulating lipolysis in vitro.


Comparative synthesis of herpes simplex virus by neonatal and mature human fibroblasts.


Tricarboxylic acids cycle dehydrogenases and lactate-dehydrogenase interaction in the amphibian embryos mitochondria.


Developmental abnormalities in the rat induced by the amino acid leucine.


Analysis of SV40-induced transformation of hamster kidney tissue in vitro. VI. Characteristics of mitomycin C induction.


The participation of sendai virus ribonucleoprotein in virus-specific polysome formation.


Transformation of mouse 3T3 cells by T antigen-forming defective SV40 virions (T particles).


Polypeptide components of virions, top component and cores of reovirus type 3.


Temperature sensitive mutants of an avian sarcoma virus.


In vitro assembly of polioviruses. I. Kinetics of the assembly of empty capsids and the role of extracts from infected cells.


Bloody ascites: diagnostic implications.


Descending monosynaptic and reflex control of gamma-motoneurones.


Cervical chemodectomas. Clinical consideration in sixteen cases.


The response of reduced pyridine nucleotide to calcium-induced alkalinity.


The origin of inorganic pyrophosphate in urine.


Genital infection with type 2 Herpes virus hominis. A commonly occurring venereal disease.


Isolated gastric parietal cells: oxygen consumption, electrolyte content and intracellular pH.


Nickel toxicity in young growing mice.

Nickel toxicity in young growing mice.


Carcinoma of the breast--a clinical study.


Intranasal removal of a tumor from the dorsum of the nose. A case report.


Current trends in the chemotherapy of brain tumors with special reference to glioblastomas.


Surgical treatment of unilateral optic nerve gliomas.


Experimental intervertebral discolysis with collagenase.


Herpesviruses and cancer.

Herpesviruses and cancer.


Rifampicin inhibits focus formation in chick fibroblasts infected with Rous sarcoma virus.


Experimental infection of gibbons with rhinovirus.


Antigenic variation among strains of rhinovirus type 51.


Evolution of the autonomic innervation of visceral and cardiovascular systems in vertebrates.


Two cases of pox in recently imported peregrine falcons (Falco peregrinus).


Nerve impulses and trophic effect. Absence of fibrillation after prolonged and reversible conduction block.


Neuropathological correlates of low-voltage EEG foci.


Peripheral nerve involvement in Fabry's disease.


Oral manifestations of primary hypophosphatasia. A case report.


Apocrine cystadenoma. A report of two cases occurring on the prepuce.


Carcinoma of the kidney.

Carcinoma of the kidney.


Treatment of Wilms' tumour: a review of 25 cases seen at the Sheffield Centre for Radiotherapy from 1950 to 1966 inclusive.


Electron microscopy of effect of polymyxin on Escherichia coli lipopolysaccharide. Polymxyin treatment of isolated lipopolysaccharide from Escherichia coli resulted in the breakdown of its structure.


Carcinoma-in-situ of the breast.

Carcinoma-in-situ of the breast.


Lactic dehydrogenases of strains of the genus Leuconostoc.


Paraganglioma.

Paraganglioma.


Carcinoma of the colon in childhood.

Carcinoma of the colon in childhood.


The avian leukosis complex.

The avian leukosis complex.


Nursing after-care in psychiatry.

Nursing after-care in psychiatry.


Competitive inhibition of uridine incorporation by 6-azauridine in uninfected and mengovirus-infected Novikoff hepatoma cells.


The effects of cyanide on oxygen consumption in bovine dental pulp.


Peroxidase-mediated virucidal systems.

Peroxidase-mediated virucidal systems. Peroxidase (myeloperoxidase or lactoperoxidase), hydrogen peroxide, and a halide such as iodide, bromide, or chloride form a potent virucidal system that is effective against polio and vaccinia viruis, particularly at a low pH. The peroxidase-halide-hydrogen peroxide system may contribute to the host defense against certain viral infections.


The growth patterns of spontaneous mouse tumors.


Seal pox. Case history.

Seal pox. Case history.


Enzymes in periodontal disease.

Enzymes in periodontal disease.


Androgen responses to stress. I. Psychoendocrine relationships and assessment of androgen activity.


Contributions to the study of myocardial involvement during viral pneumopathies of sucklings.


Family crisis intervention.

Family crisis intervention.


Action-oriented group therapy as a training method for psychiatric student nurses.


The new look.

The new look.


Education as preparation for change.

Education as preparation for change.


Politics and public care agencies.

Politics and public care agencies.


Meeting the needs of parents with a mentally retarded child.


Actual problems of significance, spreading, treatment and control.


A group therapy experience with regressed patients.


Determination of quartz. Evaluation of the differential thermal analysis method.


Effects of spino-bulbo-spinal reflex volleys on flexor motoneurons of hindlimb in the cat.


Evaluation of the effectiveness of temazepam on the insomnia of patients with neurosis and endogenous depression.


The role of the interstimulus interval in heterosynaptic facilitation in Aplysia californica.


Spontaneous and reflex discharge of a sympathetic nerve during "para-sleep" in decerebrate cat.


Pituitary-adrenal response to stress in rats with hypothalamic islands.


Relation between hypothalamic heat production and intra- and extracranial circulatory factors.


A comparative study of the role of the cerebral arterial blood in the regulation of brain temperature in five mammals.


Look-alike and sound-alike drugs.

Look-alike and sound-alike drugs.


The time course of facilitation and inhibition in the olfactory bulb, investigated with double pulse stimulation of the lateral olfactory tract.


Geriatrics.

Geriatrics.


A histochemical study of experimentally induced malignant skin tumours.


Exacerbations in chronic bronchitis.

Exacerbations in chronic bronchitis.


Latent cytomegalovirus infection in blood donors. Twenty-one out of 32 apparently healthy blood donors aged 21 to 65 years yielded positive complement fixation tests with a cytomegalovirus antigen, at titres ranging from 1:8 to 1:64. Virus was present in leucocyte cultures of fresh peripheral blood of two seropositive subjects from a total of 35 donors examined. Plasma and 48-hour stored blood specimens failed to disclose virus in culture. Viruria could not be demonstrated, and there was no evidence of recent illness in the study group. These findings suggest that subclinical viraemia is not uncommon in blood donors.


Studies on experimental autoimmune thymitis in guinea-pigs.


Effect of triamcinolone on various calcifying connective-tissue reactions.


A comparative study of different culture techniques and therapeutic efficacy of newer antibiotics in pertussis.


Otosclerosis and osteogenesis and imperfecta.


Poliovirus replicative intermediate: structural basis of infectivity.


Endocrine function in multiple sclerosis.


Response to long-term administration of human growth hormone in Turner's syndrome.


Effects of slow wave sleep deprivation on human growth hormone release in sleep: preliminary study.


Effects of gamma radiation on the chemical and physical properties of lactate dehydrogenase.


Electron spin resonance of irradiated single crystals of orotic acid.


The effects of adrenocorticotrophin hormone and metyrapone on the excretion of urinary steroids by beagle dogs burdened with strontium-90.


Histo-enzymological investigations on the muscle fibres of a cephalopode (Octopus vulg. Lam.).


Early in-vitro SV40-mediated morphologic transformation of primary hamster cells. Its correlation with the development of the oncogenic state.


An ultrastructural study of follicular cells in the human anterior pituitary.


Pathophysiology of epidemic St. Louis encephalitis. I. Inappropriate secretion of antidiuretic hormone. II. Pituitary-adrenal function. 3. Cerebral blood flow and metabolism.


Respiratory viruses.

Respiratory viruses.


An electron microscopic study of the regenerating adrenal gland during the development of adrenal regeneration hypertension.


Evaluation of stimulation and suppression tests in the etiological diagnosis of Cushing's syndrome.


Kaposi's varicelliform eruption. Two cases caused by herpesvirus hominis infection complicating Darier's disease.


Simplified feedback in neuromuscular retraining: an automated approach using electromyographic signals.


The neuromuscular blocking action of gentamicin.


A myocardial sarcolemma preparation and the ouabain-sensitive (Na plus-K plus)-ATPase.


Pharmacological studies of sleep and dreaming: chemical and clinical relationships.


Ribonucleic acid as affected by hepatocarcinogenesis.


Nucleoside phosphate levels in normal and tumour cells and their significance.


Serum zinc levels in hepatocellular carcinoma.


The renal action of angiotensin in rats after expansion of plasma volume or of extracellular fluid.


Calculation and measurement of semiconduction activation energy and electron mobility in cytochrome oxidase, with evidence that charge carriers are polarons, which may couple oxidation to phosphorylation.


Cardiovascular and respiratory changes during sleep in normal and hypertensive subjects.


Variation of killing power of human sera against Bordetella pertussis.


The utilization of exogenous pyrimidines and the recycling of uridine-5'-phosphate derivatives in Saccharomyces cerevisiae, as studied by means of mutants affected in pyrimidine uptake and metabolism.


Replication of poliovirus RNA studied by gel filtration and electrophoresis.


On the role of quinones in bacterial electron transport. The respiratory system of Bacillus megaterium.


Radical formation in cysteamine-HCl gamma-irradiated in the dry state and in frozen aqueous solution.


Structural investigations on T1 lipopolysaccharides.


Chemical synthesis and quantitative estimation of 3-deoxy D-manno-octulosonic acid.


Serum "seromucoid" and physical exercise.


Dream recall in the normal aged.

Dream recall in the normal aged.


Enzymatic synthesis of the antigen carrier lipid.


Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells. A continuous cell line of highly contact-inhibited cells (NIH/3T3) has been developed from NIH Swiss mouse embryo cultures. Its growth properties are similar to those of 3T3 and BALB/3T3. Although 3T3 is relatively insensitive to focus formation by murine sarcoma viruses, cloned lines of both NIH/3T3 and BALB/3T3 have been isolated that are highly sensitive to sarcoma virus focus formation and leukemia virus growth. The sensitivity and specificity are comparable to those found with primary embryo cells. MSV-transformed lines of NIH/3T3 have been obtained.


Simian virus 40-induced T and tumor antigens. Antigen extracts from simian virus 40 (SV40) transplanted hamster tumors were studied by rate-zonal centrifugation. Three species or molecular forms of antigen were demonstrated. The major antigen component corresponded to a molecular weight of 65,000 to 75,000, and two larger species were detectable in smaller quantities. Similar studies were carried out on SV40 virus-induced T antigen from BSC-1 cells. Three antigen components were again detected. Quantitative differences in the expression of "T" and tumor antigen species were reproducibly found.


Incorporation of precursors into ribonucleic acid, protein, glycoprotein, and lipoprotein of avian myeloblastosis virions. Freshly explanted leukemic myeloblasts produce avian myeloblastosis virus (AMV) at a constant rate without any obvious cytopathic effect; therefore, subviral components are continually synthesized at a steady rate. The incorporation of various radioactive precursors into virions was monitored by determination of radioactivity in purified virus after density equilibrium sedimentation in preformed sucrose gradients. The kinetics of incorporation of (3)H-uridine have shown that there is an average time interval of 3 to 4 hr (half-life) between the time viral ribo-nucleic acid (RNA) is synthesized and the time it is released as a mature virus particle; this represents the average time interval spent by AMV-RNA in an intracellular pool. Studies with (14)C-phenylalanine have revealed that some protein synthesis takes place at or near the cell surface immediately prior to maturation and release of virus. (14)C-glucosamine also appears to be incorporated into the outer viral envelope shortly before maturation. On the other hand, there is an average lag of about 16 to 20 hr before (14)C-ethanolamine incorporated into intracellular lipoprotein appears in free virions; this probably reflects the kinetics of replacement of cellular surface membrane. Actinomycin D inhibits AMV-RNA within 30 min but permits the maturation of AMV to continue for at least 2 hr. AMV released in the presence of actinomycin D contains AMV-RNA synthesized before the addition of the drug.


Properties of simian virus 40 rescued from cell lines transformed by ultraviolet-irradiated simian virus 40. Simian virus 40 (SV40) strains have been rescued from various clonal lines of mouse kidney cells that had been transformed by ultraviolet (UV)-irradiated SV40. To learn whether some of the rescued SV40 strains were mutants, monkey kidney (CV-1) cells were infected with the rescued virus strains at 37 C and at 41 C. The SV40 strains studied included strains rescued from transformed cell lines classified as "good," "average," "poor," and "rare" yielders on the basis of total virus yield, frequency of induction, and incidence of successful rescue trials. Four small plaque mutants isolated from "poor" yielder lines and fuzzy and small plaque strains isolated from an "average" and a "good" yielder line, respectively, were among the SV40 strains tested. Virus strains rescued from all classes of transformed cells were capable of inducing the transplantation antigen, and they induced the intranuclear SV40-T-antigen, thymidine kinase, deoxyribonucleic acid (DNA) polymerase, and cellular DNA synthesis at 37 C and at 41 C. With the exception of four small plaque strains rescued from "poor" yielders, the rescued SV40 strains replicated their DNA and formed infectious virus with kinetics similar to parental SV40 at either 37 or 41 C. The four exceptional strains did replicate at 37 C, but replication was very poor at 41 C. Thus, only a few of the rescued virus strains exhibited defective SV40 functions in CV-1 cells. All of the virus strains rescued from the "rare" yielder lines were similar to parental SV40. Several hypotheses consistent with the properties of the rescued virus strains are discussed, which may account for the significant variations in virus yield and frequency of induction of the transformed cell lines.


Variants of defective simian papovavirus 40 (PARA) characterized by cytoplasmic localization of simian papovavirus 40 tumor antigen. Three isolates of PARA (particle aiding replication of adenovirus)-adenovirus 7 out of a total of 112 clonal progeny derived by two successive plaque purifications in green monkey kidney cells (GMK) were found to induce the synthesis of simian papovavirus40 (SV 40) tumor (T) antigen in the cytoplasm of infected cells. The variant viruses induced plaque formation in human embryonic kidney cells which followed one-hit kinetics. In GMK cells, plaque formation followed two-hit kinetics which converted to first-order kinetics in the presence of additional helper adenovirus type 7. Analysis of plaque progeny from human cells showed that the progeny could replicate only in human cells, whereas progeny from monkey cells could multiply in both human and monkey cells. Heterologous human adenoviruses were able to enhance plaque formation by the variant viruses in monkey kidney cells. Neutralization tests indicated that both components of the populations had a type 7 adenovirus capsid. All three viruses were capable of inducing SV40 transplantation immunity in weanling hamsters. These results indicate the three variants are PARA-adenovirus 7 populations. Response of the induction of the synthesis of the cytoplasmic antigen to metabolic inhibitors was the same as for the synthesis of the nuclear SV40 T antigen. Different pools of sera which reacted with the intranuclear SV40 T antigen also detected the cytoplasmic antigen induced by the variant viruses. An adsorption experiment with cells containing either nuclear or cytoplasmic T antigen to remove tumor antibody from hamster sera also indicated that it is probably SV40 T antigen which is responsible for the cytoplasmic reaction. The species of the host cell-human, simian, or rabbit-appeared to play no role in the altered localization of this antigen. It is postulated that these PARA variants are further defective in some virus-mediated transport mechanism which shifts the T antigen from the cytoplasm to the nucleus.


Variation in properties of plaque progeny of PARA (defective simian papovavirus 40)-adenovirus 7. One hundred and twelve progeny from double plaque-purified clones were derived from the original PARA (defective simian papovavirus 40)-adenovirus 7 population. These progeny were found to differ in their oncogenic potential in newborn hamsters with progeny from 20 clones not inducing any tumors during 1 year of observation. The varying tumorigenicity of the individual clonal progeny was not related to the titer of PARA (particle aiding replication of adenovirus) in the inoculum. There was a perfect correlation between the tumor antigen content of the tumor cells and the antibody response of the tumor-bearing host. The tumors containing both adenovirus and simian papovavirus 40 (SV40) tumor antigens appeared earlier than those carrying only SV40 tumor antigen. Progeny from clones which induced mixed tumors also produced tumors which contained only SV40 tumor antigen. Three variants of PARA were isolated which induced the synthesis of SV40 tumor antigen in the cytoplasm of infected simian cells; all other clones yielded progeny which induced synthesis of SV40 tumor antigen in the nucleus.


In vitro transformation by adenovirus-simiam virus 40 hybrid viruses. IV. Properties of clones isolated from cell lines transformed by adenovirus 2-simiam virus 40 and adenovirus 12-simiam virus 40 transcapsidant hybird viruses. Clones were isolated from hamster cells transformed by the adenovirus 2-SV40 and adenovirus 12-SV40 transcapsidant hybrid viruses. The clones were characterized with respect to their cytomorphology, virus and antigen content, and the histomorphology of tumors induced by transplantation of the clonal sublines to hamsters. Three different cellular and colonial morphologies were observed. Clones with an SV40 morphology gave rise to tumors predominantly with an SV40 histology, whereas clones with an adenovirus morphology produced typical adenovirus tumors upon transplantation of the transformed cells. Clones which had features of both SV40 and adenovirus transformed cells gave rise to "intermediate" and adenovirus tumors. The results indicate that multiple events occur during transformation and tumorigenesis by the transcapsidant virus populations and provide an explanation for the multiplicity of findings which have been reported with these virus populations.


Host-dependent restriction of mengovirus replication. Mengovirus infection of a restrictive cell line, Maden's bovine kidney (MDBK), results in a virus yield 1,000-fold less than that obtained from productively infected cell lines such as L cells or Ehrlich ascites tumor cells (EAT). Cells of both types of host systems are infected with comparable efficiencies and are completely killed as a consequence of infection. Infective center assays, coupled with the observation of total cell killing, suggest that comparable numbers of cells synthesize viral antigen and release virus in both types of host system. Viral-specific ribonucleic acid (RNA) synthesis is initiated and proceeds in an identical fashion for approximately 4 hr after the infection of MDBK, EAT, or L-cells. At this time, viral RNA synthesis in MDBK ceases, whereas viral RNA synthesis in EAT and L-cells continues at a linear rate. These results indicate that none of the early viral events leading to the initiation of viral-specific RNA synthesis constitutes the primary site of mengovirus restriction in MDBK. Rather it appears that the cessation of viral RNA synthesis in restrictive cells constitutes the primary limiting event. Based on its delayed interaction with mengovirus RNA synthesis, it appears that the host-related restrictive agent is initially compartmentalized and then released as a consequence of infection subsequent to those early events in mengovirus infection leading to the initiation and continued synthesis of viral RNA.


In vitro transformation by the adenovirus-simian virus 40 hybrid viruses. V. Virus-specific ribonucleic acid in cell lines transformed by the adenovirus 2-simian virus 40 and adenovirus 12-simian virus 40 transcapsidant hybrid viruses. The ribonucleic acid-deoxyribonucleic acid hybridization technique was utilized to determine the presence of adenovirus (ad) and SV40 genetic information and to determine which ad genomes were present in clones of hamster cells transformed with the ad 2-SV40 and ad 12-SV40 transcapsidant hybrid virus populations. The results were correlated with the morphology of the transformed cells and colonies. It was found that cells transformed by either transcapsidant virus which had an SV40 morphology contained the ad 7 and SV40 genomes, whereas cells with a typical ad morphology contained only ad genetic information. Cells and colonies with morphological features of both ad- and SV40-transformed cells contained either the ad 2, or ad 12 genomes, depending on the transcapsidant used, together with the ad 7 and SV40 genomes. The results indicate the following: at least three different events occurred during transformation of hamster cells by the transcapsidant virus populations; the morphology of the resulting clones is determined by the viral genome(s) present; the linkage of the ad 7-SV40 genomes is confirmed since the ad 7- SV40 genomes were never found to be dissociated; the defective ad 7-SV40 genomes are capable of causing transformation; and the transcapsidant particle is probably composed of only ad 7 and SV40 genetic information.


Polynucleotide ligase activity in cells infected with simian virus 40, polyoma virus, or vaccinia virus. The conversion of simian virus 40 (SV40) component II deoxyribonucleic acid to component I has been used to assay polynucleotide ligase in extracts of tissue culture cells. All cell types examined, including chicken, hamster, mouse, monkey, and human cells, contained adenosine triphosphate-dependent ligase. After infection of mouse embryo, monkey kidney, and HeLa cells with polyoma virus, SV40, and vaccinia virus, respectively, the enzyme activity increased, but its cofactor requirement was unchanged. In vaccinia virus-infected cells, the increased activity was localized in the cytoplasm. Ligase induction occurred in the presence of cytosine arabinoside but was prevented by puromycin. Rifampicin blocked the production of infectious vaccinia particles but had little effect on the induction of ligase.


Electrophoretic characterization of foot-and-mouth disease virus-specific ribonucleic acid. Foot-and-mouth disease virus (FMDV)-specific ribonucleic acid (RNA) was analyzed by electrophoresis on 0.5% agarose gels. Four classes of RNA were resolved as a function of mobility in agarose: two classes of slowly migrating multistranded RNA, the infectious viral RNA with intermediate mobility, and a minor fast-moving class of lower-molecular-weight single-stranded RNA. The major RNA species were infectious viral RNA and the slowest migrating class of multistranded RNA. The latter RNA was polydisperse when analyzed by sucrose gradient centrifugation, it was partially ribonuclease resistant, and it was the predominant RNA species labeled during the initial period of (3)H-uridine triphosphate incorporation in the cell-free system. Heat treatment studies indicated that part of the slowest-moving RNA was degraded at 60 C and almost complete degradation was detected at 100 C. It was concluded that this RNA is the replicative intermediate in viral RNA synthesis. The second class of multistranded RNA contained both a ribonuclease-resistant RNA and a second RNA peak which was detected only after heat treatment at temperatures above 75 C. Fractions of FMDV-specific RNA isolated by sucrose gradient centrifugation were analyzed by agarose-gel electrophoresis. Infectious viral RNA was detected only in the 37S zone and was the major species of RNA in this part of the gradient. The ribonuclease-resistant RNA (the 20S zone) contained about equal amounts of multistranded RNA (both classes) and the low-molecular-weight single-stranded RNA. All sucrose gradient fractions between 20 and 40S were found to contain the replicative intermediate, although the major portion was detected in the 20 to 25S region.


Inhibition of host cell ribosomal ribonucleic acid methylation by foot-and-mouth disease virus. A study of protein and ribonucleic acid (RNA) synthesis in cells infected by foot-and-mouth disease virus has indicated possible mechanisms of viral control over host cell metabolism. Foot-and-mouth disease virus infection of baby hamster kidney cells resulted in 50% inhibition of host cell protein synthesis at 180 min postinfection. A viral-induced interference with host cell RNA methylation was observed to be more rapidly inhibited than protein synthesis. To determine the nature of methylation inhibition, the kinetics of several host cell methylated RNA species were examined subsequent to virus infection. Data from sucrose zonal centrifugation and methylated albumin kieselguhr chromatography showed that methylation of nuclear RNA was inhibited 50% at 60 min postinfection. Inhibition of nuclear ribosomal RNA precursors and formation of nascent ribosomes correlated with inhibition kinetics of nuclear RNA methylation. It is suggested that the viral interference with the host nuclear RNA methylation is directly responsible for the observed loss of nascent ribosome formation. Moreover, early in the infectious cycle, methylation inhibition of host cell RNA could, in part, account for the cessation of host protein synthesis.


Initiation of SV40 DNA-directed protein synthesis with N-formylmethionine in vitro.


Insensitivity of mitochondrial RNA synthesis to mengovirus infection in CHO cells.


Adenoid cystic carcinoma of the floor of the mouth presenting by metastases.


Intraoral minor salivary gland tumors. Report of thirty-eight cases.


Behavioral measurement of neural poststimulation excitability cycle: pain cells in the brain of the rat. A new technique in which elicited behavior of the freely moving rat is used to measure the poststimulation excitability cycle of the central neurons mediating that behavior has been adapted from accepted methods of neurophysiology. A continuous train of pairs of brief pulses was delivered to pain systems in the midbrain. Rate of lever pressing to achieve 3-second rests from this stimulation was measured as a function of the interval separating the pulses within pairs. Evidence for latent addition, absolute refractory period, temporal summation, and adaptation was demonstrated. Obtained relationships suggested that three sets of fibers may carry the aversive signal and that synaptic integration of pain in the brain may be related to Stevens' power law functions.


Adrenocortical function in patients on long-term corticosteroid treatment.


Preparation of ribosome-free membranes from rat liver microsomes by means of lithium chloride. 1. Treatment of washed rat liver microsomes in a medium containing 0.12m-sucrose, 12.5mm-potassium chloride, 2.5mm-magnesium chloride and 25mm-tris-hydrochloric acid buffer, pH7.6, with 2m-lithium chloride at 5 degrees for 16hr. leads to the formation of membranes free of ribosomes and ribosomal subunits. 2. Confirmation of the absence of ribosomes from lithium chloride-prepared membranes was obtained by treatment of the membranes with sodium deoxycholate, followed by sucrose-density-gradient centrifugation, which showed the complete absence of ribosomes. 3. Treatment of membranes with phenol, followed by sucrose-density-gradient analysis of the isolated RNA, showed the presence of a small amount of 4s material. Repetition of the phenol extraction procedure in the presence of liver cell sap as a ribonuclease inhibitor again showed the presence of only 4s material. The 4s RNA was shown to be transfer RNA by the fact that it had the same capacity for accepting (14)C-labelled amino acids as isolated transfer RNA from rat liver pH5 enzyme. 4. Analysis showed that microsomes and membranes possessed similar glucose 6-phosphatase, NADH-2,6-dichlorophenol-indophenol reductase, NADH-neo-tetrazolium reductase, NADH-cytochrome c reductase and ribonuclease activities. 5. (3)H-labelled ribosomal RNA binds to membranes. However, isolation of the bound RNA by the phenol extraction procedure, followed by sucrose-density-gradient analysis, shows the RNA to be degraded to 7s material. Very little breakdown of (3)H-labelled ribosomal RNA bound to membranes occurs if the binding and isolation are carried out in the presence of liver cell sap.


Localization of N-acetyl-beta-glucosaminidase on rat liver membranes.


The hormonal control of protein synthesis.


Enzyme distributions in an amoeba during encystment.


Lysosomes in hepatomas.

Lysosomes in hepatomas.


Lipolytic activity in the pancreas.

Lipolytic activity in the pancreas.


Interaction of salicylates with rat liver lysosomes.


The metabolism of nicotinamide-adenine dinucleotide in various classes of rat liver nuclei.


Subcellular fractionation of Saccharomyces carlsbergensis by zonal centrifugation.


The haem--protein interaction in cytochrome P-450.


The synthesis of nicotinamide-adenine dinucleotide and poly (adenosine diphosphate ribose) in various classes of rat liver nuclei. 1. The activities of NMN adenylyltransferase and an enzyme that synthesizes poly (ADP-ribose) from NAD were investigated in the various classes of rat liver nuclei fractionated by zonal centrifugation. 2. The highest specific activities of these two nuclear enzymes occur in different classes of nuclei. In very young and in mature rats it was shown that a correlation exists between DNA synthesis and NMN adenylyltransferase activity, but in rats of intermediate age this correlation is less evident. The highest activities of the enzyme that catalyses formation of poly (ADP-ribose) are in the nuclei involved in the synthesis of RNA. 3. The significance of these results in relation to NAD metabolism is discussed.


Structural studies on colanic acid, the common exopolysaccharide found in the enterobacteriaceae, by partial acid hydrolysis. Oligosaccharides from colanic acid. The exopolysaccharide slime colanic acid has been isolated from representative strains of Escherichia coli, Salmonella typhimurium and Aerobacter cloacae. Analysis showed that each polymer contained glucose, galactose, fucose and glucuronic acid, together with acetate and pyruvate. The molar proportions of these components were 1:1.8:1.9:1:1:1 approximately. On the basis of periodate oxidation of the natural and deacetylated polysaccharide, glucose is proposed as the site of the acetyl groups. The pyruvate is attached to galactose. Three neutral oligosaccharides and ten electrophoretically mobile oligosaccharides were isolated and partially characterized. Four of the fragments were esters of pyruvic acid. Most oligosaccharides were isolated from all three polysaccharide preparations. Three further oligosaccharides were isolated from carboxyl-reduced colanic acid and sodium borotritide was used to label the glucose derived from glucuronic acid in these fragments. One trisaccharide was obtained from periodate-oxidized polysaccharide. On the basis of these oligosaccharides a repeating hexasaccharide unit of the following structure is proposed: [Formula: see text] The significance of this structure in colanic acid biosynthesis is discussed.


The metabolism of D-glucarate by Pseudomonas acidovorans. 1. Dehydratases that converted d-glucarate into 4-deoxy-5-oxoglucarate were partially purified from Klebsiella aerogenes and Pseudomonas acidovorans. 2. When d-glucarate was metabolized to 2,5-dioxovalerate it appeared that water and carbon dioxide were removed from 4-deoxy-5-oxoglucarate in one enzymic step: 4,5-dihydroxy-2-oxovalerate was not an intermediate in this reaction. 3. A method for the enzymic determination of d-glucarate is described.


Influence of insulin on cyclic 3',5'-AMP phosphodiesterase activity in liver, skeletal muscle, adipose tissue, and kidney.


Effects of glucocorticoids and insulin on 3',5'-AMP phosphodiesterase activity in adrenalectomized rats.


Demonstration of N-acetyl-D-galactosamine in tumor cells by the immunofluorescence technic.


The major hydrocarbon of a cockroach cuticular wax.


An improved adenosine triphosphatase assay for physical particles of avian myeloblastosis virus (AMV).


Characterization of tumor-specific transplantation immunity reactions in immunodiffusion chambers in vivo.


Neoplastic transformation and derivation of a focus-forming sarcoma virus in cultures of rat embryo cells infected with a murine osteosarcoma (FBJ) virus.


A simple chemical method for the measurement of plasma angiotensin.


Influence of angiotensin II on the systemic and pulmonary circulations of intact anesthetized dogs.


Effects of cations and organic compounds on inactivation of poliovirus with urea, guanidine, and heat.


Inhibition of Friend virus-induced splenomegaly by an associated lymphatic leukemia virus.


Investigations on a tissue antagonist of interferon (TAI).


Propagation of mammalian viruses in protista. IV. Experimental infection of C. albicans and S. cerevisiae with polyoma virus.


Cell-mediated complementation of human adenoviruses by simian papovavirus.


Isolation of cytomegalovirus from a cohort of 100 infants throughout the first year of life.


Detection and separation of simultaneous Enterovirus and intestinal Chlamydia infection of calves.


Effect of treatment on tryptophan metabolism in childhood epilepsy.


Celo virus infection in chickens: clinical and serological response to experimental and accidental infection.


Marek's disease herpesvirus: in vivo and in vitro infection of kidney cells of different genetic strains of chickens.


Coexistence of antibodies against chronic respiratory disease, infectious laryngotracheitis, and infectious bronchitis on poultry farms of Uttar Pradesh, Andhra Pradesh, and Madras.


Serum albumin production by hepatoma cells in culture: direct evidence for stimulation by hydrocortisone.


No effect of guanidine on an improved in-vitro system for viral RNA polymerase.


The enzymatic phosphorylation of sphinganine.


On the problem of efficient antibiotic therapy of infections caused by Pseudomonas aeruginosa.


Regulation of the formation of acid phosphatases by inorganic phosphate in Aspergillus ficuum. Two types of extracellular acid phosphatases are synthesized by Aspergillus ficuum NRRL 3135: a nonspecific orthophosphoric monoester phosphohydrolase (EC 3.1.3.2) with an optimum pH of 2.0, and an enzyme with restricted specificity, a mesoinositol-hexaphosphate phosphohydrolase (EC 3.1.3.8; phytase) with an optimum pH of 5.5. Although the pH 5.5 enzyme is termed a phytase, both enzymes hydrolyze phytin. Synthesis of the enzymes is repressed by high orthophosphate concentrations in the fermentation medium. The highest total level for each enzyme is synthesized in low orthophosphate medium. In high orthophosphate medium, more pH 5.5 enzyme is produced than pH 2.0 enzyme. In low orthophosphate medium, more pH 5.5 enzyme is produced than pH 2.0 enzyme during the early stages of growth, but the reverse occurs after 5 days. The enzymes are differentiated by heat denaturation at acid and alkaline pH levels. They are separated into two distinct fractions on Sephadex G-100 followed by carboxymethylcellulose column chromatography. This indicates that the two enzymes are structurally different. The K(m) for both enzymes is 1.25 mm when calcium phytate is the substrate. Orthophosphate competitively inhibits the pH 2.0 (K(i) = 1.1 x 10(-2)m) but not the pH 5.5 phosphatase. Neither enzyme is denatured by 50% (w/v) urea or inhibited by 0.01 m tartrate. Thus, they differ from human prostatic phosphatase.


Carbohydrate transport in Clostridium perfringens type A. Resting-cell suspensions of Clostridium perfringens actively transported glucose and mannose but not six other carbohydrates which would support at least limited growth of the microorganism. Competition studies suggested that glucose and mannose were transported by a common mechanism which was highly specific. Analyses of intracellular pools demonstrated that the accumulated carbohydrates were present as phosphorylated derivatives, not as free carbohydrates, and specificity data indicated that phosphorylation occurred at the transport level rather than via the soluble hexokinase.


Streptolydigin, an inhibitor of oxidative phosphorylation in rat liver mitochondria. Streptolydigin interferes with oxidative phosphorylation in rat liver mitochondria. The agent acts primarily as an uncoupler of respiration-associated phosphorylation but also impairs respiration to various degrees depending on the substrate. Streptolydigin partially inhibits electron flow at a point past the cytochrome b and prior to the cytochrome c reduction site. Streptolydigin also inhibits the function of the enzyme ribonucleic acid polymerase in whole bacterial cells and cell-free systems. The streptolydigin concentrations that cause effective inhibition of ribonucleic acid polymerase in cell-free systems are approximately 10 times less than those required to inhibit oxidative phosphorylation in mitochondria.


Phosphorylation of methyl-alpha-D-glucopyranoside in polymyxin B-treated Salmonella typhimurium. Phosphoenolpyruvate-dependent phosphorylation of methyl-alpha-d-glucopyranoside in Salmonella typhimurium is increased by the membrane active polypeptide antibiotic polymyxin B whereas active transport ability is abolished.


Glucose degradation in Clostridium perfringens type A. Cell-free extracts of Clostridium perfringens were found to contain all the enzymes of the Embden-Meyerhof pathway of glycolysis in addition to lactic acid dehydrogenase and the pyruvate-clastic system. Neither glucose-6-phosphate dehydrogenase nor 6-phosphogluconate dehydrogenase activities could be demonstrated, suggesting the absence of the conventional hexose-monophosphate pathway in this organism.


Transfer RNA from tumor tissue.

Transfer RNA from tumor tissue.


An enzyme histochemical study of some dehydrogenases in abnormal human mammary tissue.


Pink cell (oncocytic) metaplasia in a fibro-adenoma of the human breast: electron-microscope observations.


Changes in the reactivity of the skin blood vessels of the rabbit with age.


Management of foot drop in adults after permanent peroneal nerve loss.


Natural selection of long-lived macrophages in experimental granulomata.


Early morphological and histochemical consequences of peripheral nerve section in lambs of normal and low copper status.


An electrophysiological study of the innervation of the smooth muscle of the colon. 1. Micro-electrodes were used to examine transmission from intrinsic and extrinsic nerves to single cells of the longitudinal and circular muscle coats of colons from guinea-pigs and rabbits.2. When intrinsic nerves were stimulated, inhibitory junction potentials (IJPs) were observed in over 90% of the cells of the longitudinal coat and nearly all of the cells of the circular coat. The characteristics of IJPs in the guinea-pig and rabbit were similar. Facilitation of two or three successive IJP's was sometimes observed. The IJPs were unaffected by guanethedine but were blocked by procaine. IJPs could not be elicited by stimulation of either the pelvic parasympathetic or periarterial sympathetic nerves. In some cells, the IJP was preceded by a brief depolarization, which probably results from the stimulation of excitatory nerves to the same cell.3. Excitatory junction potentials (EJPs) were observed in about 5% of cells of the longitudinal coat in response to stimulation of the intramural nerves. When the pelvic nerves were stimulated, EJPs were observed in most cells of both the longitudinal and circular muscle coats. The EJP in the guinea-pig colon was blocked by hyoscine.4. The interaction of the IJP evoked by intrinsic nerve stimulation and the EJP evoked by pelvic nerve stimulation was examined. When both nerve supplies were stimulated together, only an IJP was observed.5. The nature of the innervation of the colon and the origin of intrinsic non-adrenergic inhibitory neurones is discussed.


Transmission from vasoconstrictor and vasodilator nerves to single smooth muscle cells of the guinea-pig uterine artery. 1. Previous studies have shown that perivascular nerve stimulation of the uterine artery of the guinea-pig evokes an adrenergic constrictor response and a dilator response with two components. The first of these, only present during pregnancy, is cholinergic. The second is non-cholinergic and is present at all times. Intracellular recording from single smooth muscle cells in isolated arterial segments has now been used to investigate the transmission processes associated with these responses.2. The mean resting membrane potential of the muscle cells was 60.7 mV in arteries from both virgin animals (range 50-68 mV) and from animals in late pregnancy (range 48-76 mV).3. Low frequency perivascular stimulation evoked excitatory junction potentials (EJPs) which reached a maximum amplitude of about 5 mV, lasted about 900-1000 msec, and showed facilitation at frequencies of stimulation of 0.1 Hz or above and summation at frequencies of stimulation of 1.2 Hz or above.4. These EJPs were abolished by exposure of the tissue to bretylium (2 x 10(-6) g/ml.). It is therefore concluded that the EJPs were due to transmission from adrenergic nerves.5. Perivascular stimulation at frequencies above 10 Hz evoked a depolarizing response which was often surmounted by a small (5 mV) local spike potential. Such depolarizing responses were associated only with localized contractions of the arterial muscle.6. In the presence of low extracellular K(+) concentrations, perivascular stimulation at frequencies above 10 Hz gave rise to a depolarizing response topped by an action potential of up to 50 mV amplitude, and more generalized contraction of the tissue than was seen in normal K(+) solution.7. After blockade of the adrenergic vasoconstrictor fibres, no response to perivascular stimulation was observed normally. However, following moderate depolarization of the membrane with noradrenaline, stimulation evoked a hyperpolarization of up to 6 mV in amplitude.8. This response showed no discrete junction potentials, had a latency of up to 2000 msec and was only observed with stimulation at frequencies of 2 Hz or greater. The response was obtained in both pregnant and non-pregnant animals, and was unaffected by hyoscine, but was abolished by cinchocaine.9. No changes in membrane potential attributable to transmission from cholinergic dilator nerves could be revealed in arteries from pregnant animals. Furthermore, high concentrations of acetylcholine had no polarizing effect on the muscle cells. It is suggested that the cholinergic dilator nerves may not act via changes in membrane potential.


Insulin biosynthesis and secretion--a still unsettled topic.


Adrenocorticotrophic-hormone deficiency in chronic myelogenous leukemia after treatment.


Trigeminal sensory neuropathy and bell's palsy.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 4-1970.


Diagnosis and treatment of trophoblastic disease. A report from the Southeastern Regional Center.


Carcinoma of the vulva. Influence of radical operation on cure rate.


Antigens of virus-induced tumors.

Antigens of virus-induced tumors.


Sleep loss and cerebral excitability.

Sleep loss and cerebral excitability.


Action of curare and magnesium on striated muscle of stress-susceptible pigs.


Dynamics of uterine circulation in pregnant and nonpregnant sheep.


Effects of changes in serum calcium on hypocalcemic response to thyrocalcitonin in the rat.


Automated measurement of phosphatases.

Automated measurement of phosphatases.


Anaerobic reductive titrations with solid diluted sodium dithionite in an apparatus suitable for EPR spectroscopy.


Atypical pneumonia in young men with rhinovirus infections.


Potential medical problems in personnel returning from Vietnam.


Unusual clinical manifestations and angiographic findings in a patient with the Zollinger-Ellison syndrome.


The fibrinogen-fibrin system in human intracranial tumor tissues: an immunohistological study.


Fractionation of deoxyribonucleic acid from multiple-drug-resistant staphylococci on poly-L-lysine-kieselguhr columns.


New in psychiatry. Moditen injectable therapy and follow-up care.


The role of cyclic AMP in the control of carbohydrate metabolism.


Significance of human plasma insulin Sephadex fractions.


Effects of prostaglandin E-1 on the hypothalamo-hyophyseal-adrenocortical axis in rats.


Effects of thyroid hormones on the guinea pig.


Absorption and conjugation of metyrapone during diphenylhydantoin therapy: mechanism of the abnormal response to oral metyrapone.


Production of steroids by in vitro superfusion of endocrine tissue. II. Steroid output from bisected whole, capsular and decapsulated adrenals of normal intact, hypophysectomized and hypophysectomized-nephrectomized rats as a function of time of superfusion.


Enzymatic activities of cortisol hydroxylation at C-2a and C-6 beta in liver, adrenal and spleen homogenates of L2C-NB leukemia-bearing guinea pigs.


Iodine metabolism in thyroid slices: effects of TSH, dibutyryl cyclic 3',5'-AMP, NaF and prostaglandin E-1.


Failure of "bound insulin" to affect glucose metabolism of brown fat cells.


Acute effects of adrenocorticotropin on the levels of various lipids in the adrenals of hypophysectomized rats.


Effects of ACTH and an inhibitor of 3 beta-hydroxysteroid dehydrogenase on the synthesis of adrenocortical mitochondrial proteins.


Determination of elastolytic activity with 125-I and 131-I labelled elastin.


Uridine diphosphoacetylglucosamine pyrophosphatase activity during connective tissue growth.


Hydrolysis of condensed phosphates by a partially purified phosphatase from cactus phylloclades.


A study of the mechanism of electron transfer in cytochrome c. Chromium as a probe.


Studies on cytochrome c peroxidase. XVI. Recombination of apoenzyme with spin-labeled protoheme.


Studies on poly adenosine diphosphate-ribose. VII. Methods of separation and identification of 2'-(5"-phosphoribosyl)-5'-adenosine monophosphate, ribosyladenosine monophosphate, and phosphoribosyladenosine.


Partial resolution of the enzymes catalyzing oxidative phosphorylation. XX. Characterization of ASU-particles.


Electron paramagnetic resonance study of the stereochemistry of nitrosylhemoglobin.


Partial resolution of the enzymes catalyzing oxidative phosphorylation. XXI. Resolution of submitochondrial particles from bovine heart mitochondria with silicotungstate.


Derivatives of 2,3,4,5,6-penta-O-benzyl-myo-inositol.


Partial resolution of the enzymes catalyzing oxidative phosphorylation. XIX. Purification and characterization of a new coupling factor (F5).


Studies on Japanese B encephalitis virus vaccines from tissue culture. X. Characterization of a 37 degreeC passage line attenuated strain of OCT-541 virus.


A histochemical study of enzyme changes and ultrastructure of the jejunal mucosa in protein-depleted rats.


Electron microscopic changes in mitochondria of the intestinal epithelium and the liver in mice and D-avitaminotic rats after peroral distribution of sodium fluoride.


Cochlear blood flow in response to vasodilating drugs and some related agents.


Control of the reversion of properties in transformed cells.


In vivo replication of normal and tumor cells: relation to cancer chemotherapy.


Chemospecific synaptic repression as a possible memory store.


Translation of RNA from encephalomyocarditis virus in a mammalian cell-free system.


Reactions to a questionnaire survey: some sources of respondent resistance.


Spin-label study of the iodine-lecithin interaction.


Stimulation by cyclic AMP of intrinsic protein kinase activity in ox brain membrane preparations.


Cyclic 3',5'-AMP and uterine contractility.


Salivary gland enlargement and functional changes during feeding of pancreatin to rats. (Possible relation to functional changes in cystic fibrosis).


Proinsulin and the biosynthesis of insulin.


Metabolic events associated with steroid hydroxylation by the adrenal cortex.


Some recent neurophysiological contributions to the problem of brain and behavior.


The development of the cerebellum of vertebrates in relation to the control of movement.


Activation of glutathionase in rat liver during carcinogenesis.


Dry mass of human viruses, as determined by quantitative electron microscopy.


Chromophobe adenomas of the pituitary gland in Cushing's disease.


The growth of the ventral prostate, the dorsolateral prostate, the coagulating glands and the seminal vesicles in castrated male rats injected with ACTH and-or insulin.


Studies on the mode of action of alpha-methyldopa.


An automated fluorometric alkaline phosphatase microassay with 4-methylumbelliferyl phosphate as a substrate.


Cardiopulmonary function in uranium miners.


Cellular aspects of the control of physiological color changes in crustaceans.


Cellular aspects of the control of physiological color changes in fishes.


Cellular aspects of the control of physiological color changes in amphibians.


Physiological color changes in reptiles.


Granular cell myoblastoma of the larynx.


Benign tumors of lung and tracheobronchial tree.


Granular cell myoblastoma of the tongue.


Ion-dependent activation and inhibition of ribonucleotide reductase from Lactobacillus leichmannii.


Phosphate and mannose transfer from guanosine diphosphate mannose to yeast mannan acceptors.


Congenital cytomegalovirus infection: clinical, pathological and virological studies of two fatal cases. Two infants severely ill at birth as a result of congenital cytomegalovirus infection were born in Halifax, Nova Scotia, in April and July 1968. Both died within seven weeks. Clinical, pathological and virological studies including serology and electron microscopy were made both before and after death. Cytomegalovirus was isolated in tissue culture from urine specimens from both infants during life and from organ tissues, blood and ascitic fluid after death. Large inclusion-bearing cells mixed with the erythrocytes and leukocytes were seen within the lumina of pulmonary blood vessels. A brief general discussion is presented of the various clinical manifestations of congenital cytomegalovirus infection and of the different serum antibody patterns found in these two pairs of mothers and infants.


Positive inotropic effects of dibutyryl cyclic adenosine 3',5'-monophosphate.


Prevalence of cytomegalovirus infection in a normal urban population in Nova Scotia. During 1967 and 1968 a seroepidemiological survey was conducted on the prevalence of CF antibodies to CMV in the normal population of the Halifax-Dartmouth metropolitan area of Nova Scotia. Samples of 550 sera, collected from all age groups, including newborns, were processed. At birth, 34% of infants possess antibodies, presumably of maternal origin, and there follows a decline until at 2 years of age only 4% of children exhibit evidence of infection. There is a gradual increase up to 16% by 20 years of age, and thereafter this is maintained until by 40+ years half the population possesses antibodies.A more detailed analysis of cord sera indicated that approximately 60% of women of childbearing age possess no antibodies and are susceptible to primary CMV infection. Among these, age and gravidity are not significant factors.These data indicate that CMV infection is fairly widespread in this community, although comparative studies suggest that it is less so than in some other areas, such as Easter Island, where a more homogeneous pattern of overcrowding and poor sacioeconomic conditions prevails.


Susceptibility of dinucleotides bearing either 3'- or 5'-monophosphate to micrococcal nuclease.


Alterations in the characteristics of sugar uptake by mouse cells transformed by murine sarcoma viruses.


Interaction of acetylcholine with different cholinergic nerve receptors.


Windborne dispersal of foot and mouth virus.


Cell size, macromolecule composition, nuclear number, oxygen consumption and cyst formation during two growth phases in unagitated cultures of Acanthamoeba castellanii.


Cerebral gigantism: concentrations of amino acids in plasma and muscle.


Suppression of pituitary-adrenal function with physiological plasma levels of corticosterone.


Vincristine therapy in an infant with probable hepatoblastoma.


The effect of a CRF preparation on ACTH release in rats bearing hypothalamic dexamethasone implants: a study on the "implantation paradox".


Hereditary adrenocortical unresponsiveness to ACTH.


Antibiotic guidelines for hand infections.


Angiographic investigations in portal hypertension.


Effects of ovariectomy and hypervitaminosis A on lysosomes of the rat conceptus.


Angiofibromatous polyp of the pharynx.

Angiofibromatous polyp of the pharynx.