Biosynthesis of squalene and cholesterol by cell-free extracts of adult rat brain. Cell-free extracts of adult rat brain incubated with mevalonic acid-2-(14)C synthesize (14)C-labeled nonsaponifiable fractions consisting largely of squalene-(14)C. If the cofactor concentrations of the incubation medium are adjusted, much of the squalene can be induced to undergo turnover, with a resultant increase in (14)C-labeled digitonin-precipitable sterols, which include a small amount of cholesterol. The synthesis of labeled sterols is markedly increased in the presence of Mg(++) and depressed by nicotinamide. ATP, NADH, GSH, and glucose-6-phosphate are required for optimal synthesis of digitonin-precipitable material but, unlike Mg(++), are not essential. The cofactor-adjusted extracts also synthesize a complex ester mixture containing, in addition to cholesterol-(14)C, several compounds less polar than cholesterol. The biosynthesis of cholesterol in the extracts is a slow process; at least 12 hr of incubation is required for maximal sterol biosynthesis. A complex mixture of hydrocarbons accompanies squalene in the incubated extracts.


Aetiology of ulcerative colitis.

Aetiology of ulcerative colitis.


Phospholipid metabolism during bacterial growth. Haemophilus parainfluenzae incorporates glycerol and phosphate into the membrane phospholipids without lag during logarithmic growth. In phosphatidyl glycerol (PG), the phosphate and unacylated glycerol moieties turn over and incorporate radioactivity much more rapidly than does the diacylated glycerol. At least half the radioactivity is lost from the phosphate and unacylated glycerol in about 1 doubling. The total fatty acids turn over slightly faster than the diacyl glycerol. In phosphatidyl ethanolamine (PE), which is the major lipid of the bacterium, ethanolamine and phosphate turn over and incorporate radioactivity at least half as fast as the phosphate in PG. The glycerol of PE did not turn over in 4 bacterial doublings. In phosphatidic acid the glycerol turns over at one-third the rate of phosphate turnover. By means of a modified method for the quantitative recovery of 1,3-glycerol diphosphate from cardiolipin, the phosphates and middle glycerol of cardiolipin were shown to turn over more rapidly than the acylated glycerols during bacterial growth. There is no randomization of the radioactivity in the 1- and 3-positions of the glycerol in the course of 1 doubling. The fatty acids of PG turn over faster than those in PE. In both lipids the 2-fatty acids turn over much faster than the 1-fatty acids. At both positions the individual fatty acids have their own rates of turnover. The distribution of fatty acids between the 1- and 2-positions is the same as in other organisms, with more monoenoic and long-chain fatty acids at the 2-position. The different rates of turnover and incorporation of radioactivity into different parts of the lipids suggest that exchange reactions may be important to phospholipid metabolism.


Phosphorus determination in phosphoglycerides from thin-layer chromatograms. Phosphoglyceride phosphorus is readily determined directly on silica gel removed from thin-layer chromatograms, without elution, by a nondigestive procedure with a sulfuric-periodic acid reagent. The method is specific for hydrolyzable phosphoglyceride containing two acid-hydrolyzable groups. Cariolipin reacts only partially, while sphingomyelin, diether lecithin, and phosphonate cephalin fail completely to react.


Evaluation of radiotherapy of tumors in the pineal region by ventriculographic studies with iodized oil.


Intracranial arteriovenous malformation, pulmonary arteriovenous fistula, and malignant glioma in the same patient. Case report.


Alveolar cell (bronchiolar) carcinoma of the lung.


Renal cortical tubular glycogen localization in glycogenosis type II (Pompe's disease).


Cytoplasmic RNA synthesis in adrenal: rapid, selective stimulation by adrenocorticotropic hormone (ACTH).


Adenosine 3',5'-monophosphate stimulation of uterine amino acid uptake in vitro.


Interaction of ouabain with drugs activating lipolysis in vitro.


Adenyl cyclase and adenosine 3',5'-cyclic phosphate phosphodiesterase in the receptor tissues of neurohypophysial hormones.


Relation of fatty acid oxidation tgluconeogenesis: effect of pentenoic acid.


The paradoxical dexamethasone reponse phenomenon.


Instrumentation for study of neuroregulatory agents and behavior.


Descriptive epidemiology of interstitial pneumocystic pneumonia. An analysis of 107 cases from the United States, 1955-1967.


Respiratory virus vaccines. 8. Field evaluation of trivalent parainfluenza virus vaccine among preschool children in families, 1967-1968.


Lung perfusion in bronchiolo-alveolar carcinoma. A case report.


Bovine para-influenza 3 vaccine studies. A bovine para-influenza 3 modified live virus vaccine was studied in colostrum-deprived calves. A single dose of vaccine induced antibody responses and afforded protection against experimental challenge. This was evidenced by reduced clinical disease and virus excretion as compared to unvaccinated control calves. The vaccine virus did not spread from vaccinated calves to contact controls.


Immunofluorescent studies of bovine para-influenza 3 virus. II. Experimentally infected calves. Fluorescent antibody (FA) studies of tissues from three colostrum deprived calves inoculated intranasally with the SF-4 strain of bovine para-influenza 3 (PI-3) virus indicated that these calves developed a mild upper respiratory infection but infected cells were not identified in the lower respiratory tract. Three other calves inoculated intranasally and intratracheally with PI-3 virus developed more severe clinical signs of infection and virus was identified, by FA techniques, in the upper and lower respiratory tract of all three calves and in the spleen of one calf. PI-3 virus was detected in smears of nasal epithelium from five of six calves at some time during the observation period.


Equine infectious anemia: preliminary investigation of the complement-fixation test for the demonstration of antibodies and antigen. Clinical field cases of equine infectious anemia were studied and the disease was reproduced experimentally in horses. Attempts were made to adapt the complement-fixation test to the detection of antibodies in the serum of infected animals and to the demonstration of antigens in tissue extracts.A moderate complement-fixing antibody response was demonstrated in the serum of horses shortly after primary exposure to the infectious agent. However, this reactivity was of short duration and occurred with normal as well as with infected saline tissue extracts. It was therefore concluded that this reaction was not specific for equine infectious anemia. Possibly it is due to the appearance of auto-tissue antibodies. The value of this reaction in the diagnosis of the infection was limited because of its short duration and absence in chronic infection and following re-exposure to the infectious agent.


Immunofluorescent studies of bovine para-influenza 3 virus. I. Cell cultures and experimentally infected hamsters. Immunofluorescent techniques were used to follow the replication of the SF-4 strain of bovine para-influenza 3 (PI-3) virus in Madin-Darby Bovine Kidney (MDBK), secondary bovine kidney (BK), Embryonic Bovine Trachea (EBTr) cell cultures and experimentally infected hamsters. The virus replicated equally well in MDBK and secondary BK cell cultures but less successfully in EBTr cultures. Nuclear fluorescence was not observed in the cell systems studied. Infection was limited to the epithelial lining of the upper respiratory tract, trachea and bronchi of experimentally infected hamsters. Virus was most easily identified in smears prepared from turbinate mucosa suggesting a potential diagnostic technique for use in identifying PI-3 infected animals.


Antibody production in milk serum after virus instillation of goat mammary gland. 3. Response to herpes simplex virus. Three attempts were made to propagate HERPES SIMPLEX virus in the mammary gland of goats. No active virus resulted. In one instance a marked clinical response was induced in the portion which had been instilled. Repeated instillations induced the synthesis of neutralizing antibody.


A serum neutralization test for infectious bovine rhinotracheitis based on colour reaction and cytopathic effects in cell culture. A serum neutralization (SN) test based on a combination of indicator colour change in medium and cytopathic (CP) effect in cells has been devised for the detection of infectious bovine rhinotracheitis antibodies. Serum dilutions of 1:6, 1:18 and 1:54 are made in a medium containing phenol red and are mixed in equal quantities with a suspension of virus containing 100 cell culture infectious doses (CCID(50)) per volume of mixture. The serum-virus mixtures are held in small glass tubes and are covered with a layer of mineral oil. Following a two hour period of incubation at 37 degrees C a quantity of bovine fetal kidney cells is added to each tube to detect the presence of unneutralized virus. After four to six days incubation the results of the SN test may be read by microscopic examination for CP effect by means of an inverted microscope, or by observing the colour of the phenol red.


Glycogen in the normal human testis: a histochemical and histoenzymatic study.


The delta5-3beta-hydroxysteroid dehydrogenase of bovine adrenal microsomes.


The effect of lysine vasopressin and hypothalamic extracts on the rate of corticosterone secretion in rats treated with dexamethasone and pentobarbitone.


The maintenance of lactation in the rabbit after hypophysectomy.


Ovarian dysfunction associated with an anticonvulsant drug.


The many facets of alveolar-cell carcinoma of the lung.


Arterial portography: patterns of venous flow.


Phosphorylation of protein in liver and kidney mitochondria of the rat.


On the inhibition mechanisms of viomycin synthesis by inorganic phosphate.


Comparative histochemical studies on the inflammatory and-or vitral reaction after infliction of incised wounds in the skin and adipose tissue of guinea-pigs.


Electron microscopic observations on glioblastoma.


Transmission from pregnanglionic fibres in the hypogastric nerve to peripheral ganglia of male guinea-pigs. 1. Intracellular records were obtained from ganglion cells of the pelvic plexus of male guinea-pigs.2. The input resistance of cells which responded to intracellular stimulation varied from 40 to 150 MOmega. Slope resistance decreased when the membrane was hyperpolarized. Time constants varied from 5 to 200 msec. Resting membrane potentials ranged from 40 to 70 mV.3. Action potentials in response to direct stimulation were followed by a prolonged phase of after-hyperpolarization.4. A second type of cell was also impaled which did not respond to electrical stimulation. These cells had resting membrane potentials in the range 60-70 mV, input resistances of less than 20 MOmega and time constants of less than 3 msec.5. In most ganglion cells, stimulation of the hypogastric nerve evoked action potentials which were often followed by a secondary phase of depolarization indicating continuing transmitter action.6. Orthodromic responses were generally ;all-or-nothing' and could not be graded with changes in stimulus strength. The latency of orthodromic responses indicated that ganglion cells were innervated by both B and C fibres in the hypogastric nerve.7. Orthodromic responses were blocked by tubocurarine, 5 x 10(-5) g/ml., and dihydro-beta-erythroidine, 10(-5) g/ml.8. Spontaneous, excitatory post-synaptic potentials of up to 4.8 mV in amplitude were observed. The frequency of their discharge was greatly increased by repetitive stimulation of the hypogastric nerve.9. The ultrastructure of the pelvic ganglia was studied by electronmicroscopy. Two types of ganglion cell process were observed, fine (0.1 mu) branching tufts thrown up from the soma within the surrounding capsule and longer, thicker (1 mu) extracapsular processes. Synapses were found to occur most frequently between the varicose terminal segments of preganglionic axons and the small intracapsular processes.10. Similarities between the properties of the pelvic ganglia innervated by the hypogastric nerve and those of the parasympathetic division of the autonomic nervous system are discussed.


Acetylcholine and related enzymes in the neural lobe and anterior hypothalamus of the rabbit. 1. Acetylcholine (ACh), cholinesterases and choline acetyltransferase (choline acetylase) were estimated in the neural lobe and hypothalamus of the adult male rabbit. Acetylcholine was also estimated in the neural lobes and hypothalami of some other mammals.2. Acetylcholine-like activity was measured by bio-assay using the leech dorsal muscle preparation.3. Characterization experiments indicated that about 90% of the activity measured was due to acetylcholine.4. Mean acetylcholine content in the neural lobe of the rabbit, after extraction with perchloric acid, was 4.38 +/- 0.98 mug/g fresh tissue, and 4.87 +/- 1.53 mug/g in the hypothalamus.5. Acetylcholine was also found to be present, in comparable concentrations, in the neural lobe of man and in the neural lobes and hypothalami of ox, rat and hedgehog.6. Acetylcholinesterase, present in the neural lobe and hypothalamus of the rabbit, hydrolysed 1.74 +/- 0.11 mu-moles of substrate/min/g and 3.78 +/- 0.60 mu-moles substrate/min/g fresh tissue respectively.7. The concentration of butyrylcholinesterase was about one tenth that of acetylcholinesterase in both tissues.8. Choline acetyltransferase present in the neural lobe and in the hypothalamus synthesized 87 +/- 22 mug ACh/hr/g fresh tissue and 378 +/- 149 mug respectively.


Fractionation of equine infectious anemia virus by diethylaminoethyl cellulose chromatography and sucrose density gradient centrifugation.


Characteristics of the complement-fixing antigen of equine infectious anemia virus.


A perfused vein preparation sensitive to plasma kinins.


Congenital anomalies in children with malignancy.


Electromyogram suppression during sleep, dream recall, and orientation time.


The contribution of adrenergic mechanisms to frequency potentiation and to paired stimulation in guinea pig isolated atrial tissue.


Vertical eye-movement during REM sleep: effects of age and electrode placement.


Some effects of vitamin D 3 on collagen synthesis in rachitic chick cortical bone.


Toxicologic investigations on a new anti-arrhythmic compound: 1,5-dimorpholino-3-(l-naphthyl)-pentane (DA 1686).


Hemodynamic responses to vasopressin before and during infusion of sympathomimetic amines and angiotensin in anesthetized dogs.


The combined effect of chlorprothixene and 4-hydroxy-5-fluoropyrimidene on the protein synthesis of various tumour cells.


Antibodies to Herpesvirus hominis types 1 and 2 in the rabbit.


Sleep-induced behavioral response. Relationship to susceptibility to hypnosis and laboratory sleep patterns.


The sleep characteristics of the normal aged male.


Some correlates of rapid eye movement sleep in the normal aged male.


Inhibition by gamma-hexachlorocyclohexane of acetylcholine-stimulated phosphatidylinositol synthesis in cerebral cortex slices and of phosphatidic acid-inositol transferase in cerebral cortex particulate fractions.


Distribution and properties of CDP-diglyceride:inositol transferase from brain.


Biochemical changes in beta, beta'-iminodipropionitrile-treated rat brain and prevention of toxicity by ethionine.


Indeterminacy, the synapse, the mnemic microstate, and the psyche.


A comparative study of brain and liver mitochondria from new-born and adult rats.


Ultrastructural localization of inosine-5'-diphosphatase and glucose-6-phosphatase activities in the crayfish vas deferens.


The lymphomas of Brazilian children.

The lymphomas of Brazilian children.


The geographic pathology of children's lymphomas.


Sleep now, pay later.

Sleep now, pay later.


Exchange coupling in two dimeric copper adenine complexes.


Antilipolytic effect of N6,2'-O-dibutyryl-3' ,5'-adenosine monophosphate in vivo.


Problem solving in arranging for comprehensive home care.


Growth and regression of rabbit tumours induced by polyoma virus.


Postoperative gas gangrene.

Postoperative gas gangrene.


Sources of gas gangrene in hospital.

Sources of gas gangrene in hospital. Of four cases of postoperative gas gangrene in three hospitals three followed amputation of legs with gangrenous lesions, and one followed gastrectomy. Clostridium welchii was isolated from the wounds and the faeces of each patient; small numbers of Cl. welchii were found on the floors of the theatres where the operations had been performed.Two infections occurred in one hospital on successive days. Typing of strains of Cl. welchii from these patients showed that they were serologically distinct. Further studies suggested that in each of the four cases infection was probably acquired from the patients' intestinal flora, probably through faecal contamination of skin.In 76 patients sampling of the skin with surface contact plates showed occasional heavy contamination of the thighs, groins, and buttocks with Cl. welchii, most of which were present as spores or sporing bacilli; Cl. welchii was more commonly found in patients with incontinence of faeces. Compresses of povidone-iodine applied for 30 minutes were found greatly to reduce the numbers of Cl. welchii, and swabbing with 70% alcohol was effective in some cases; washing with soap and water had no effect on the numbers of Cl. welchii on the skin.


Responsiveness of enzymes in liver to growth of Novikoff hepatoma.


Androgens and growth.

Androgens and growth.


Allergic intolerance to tetracosactrin.

Allergic intolerance to tetracosactrin.


The effect of 1, 1-dimethyl-4-phenylpiperazinium iodide on transmission in the superior cervical ganglion of the cat.


The potentiating influence of imipramine on ganglionic effects of catecholamines.


Molecular and crystal structure of viomycidine.


The effect of atropine and curarine on the time course of the end-palate potential in frog sartorius muscle.


Sulfomycins, a series of new sulfur-containing antibiotics. I. Isolation, purification and properties.


Long biological half-life of vitamin D3 and its polar metabolites in human serum.


St. Louis encephalitis in St. Louis County, Missouri, during 1966.


The production of sustained hypertension in dogs by a single transient anoxic episode.


Studies on cytochrome oxidase. IV. Optical rotatory dispersion of hematin a.


Studies on cytochrome oxidase. V. Factors affecting the optical rotatory dispersion of hematin a.


Characterization and quantitative determination of the hydroxylysine-linked carbohydrate units of several collagens.


Inhibitory effect of physiological bicarbonate ion levels on the activities of glucose 6-phosphate phosphohydrolase.


Reaction of myosin with 1-fluoro-2, 4-dinitrobenzene at low ionic strength.


Pyrophosphate binding to and adenosine triphosphatase activity of myosin and its proteolytic fragments. Implications for the substructure of myosin.


The structure of a glycopeptide from human orosomucoid (alpha-acid glycoprotein).


220 MHz nuclear magnetic resonance spectra of oxidized and reduced pyridine dinucleotides.


Measurement of binding of phosphate ligands to ribonuclease A by direct and competive dialysis rate techniques.


Innervation and neural excitation of ventral muscle fibres of the larva of the waxmoth, Galleria mellonella.


Chemical and ultrastructural identification of 5-hydroxytryptamine in an identified neuron. The two largest cells in a typical ganglion of the leech (Hirudo medicinalis) nervous system are the colossal cells of Retzius. These cells show a positive chromaffin reaction, and it has been suggested that they contain 5-hydroxytryptamine (5-HT). In this study, the presence of 5-HT in the colossal cells was confirmed by microspectrofluorometry and by thin-layer chromatography and spectrofluorometry of extracts of individually dissected and pooled colossal cell bodies. A single colossal cell body was found to contain, on the average, 3.8 x 10(-10) g (6mM) 5-HT. Electron microscopy shows that the colossal cells are distinguished by the presence of 1000 A granules with irregular, electron-opaque cores. Since the granules are distributed in the same pattern as the 5-HT fluorescence, we have suggested that they contain 5-HT. Furthermore, a chromaffin reaction modified for the electron microscope provides evidence that 5-HT is present in the granule cores. These data can now serve as a basis for further studies on the metabolism, distribution, and function of 5-HT in these identified neurons.


The distribution of factor S in the cockroach, Periplaneta americana, and its role in stress paralysis.


The morning recall of rapid eye movement period reports given earlier in the night.


The effects of hearing one's own voice on dream content: a replication.


The influence of ACTH and corticosteroids on the rhythm of adrenocortical function in the 10-day-rebound-phenomenon.


Disturbances of plasma cortisol circadian rhythm and of cortisol-ACTH feedback mechanism in central nervous system diseases.


Circadian rhythmicity of pituitary adrenal function under resting and stress conditions in rats.


Growth studies of calcium oxalate in the presence of various ions and compounds.


Inhibition of glycolysis in Ehrlich ascites tumor cells incubated with formimino-L-glutamate.


Effects of adenosine triphosphate and inorganic pyrophosphate on carbon dioxide fixation by isolated spinach chloroplasts.


The present status of the diagnosis and treatment of periphlebitis retinae (Eales' disease).


A soluble factor related to the energy-linked transhydrogenase reaction of Rhodospirillum rubrum chromatophores.


Magnetic resonance studies of the interaction of cupric ion with native and modified forms of ribonuclease.


A case of mixed salivary tumour of the palate.


Blood flow through organs of the rat after an intravenous injection of agniotensin.


Fatal varicella generalisata in a child with immunopathy and hereditary neurological syndrome. A report with autopsy, electron microscopy and virus isolation.


Melanosis of sheep liver. I. Chemistry of the pigment.


Inactivation of foot-and-mouth disease virus in milk.


Catalysis of disulfide interchange in acid media by selenium and tellurium oxy acids.


Studies on poisoning by Macrozamia communis. II. Effects of modifying agents upon changes induced in the liver by Macrozamia communis.


The influence of pyrophosphate analogues (diphosphonates) on the precipitation and dissolution.


Characteristics of after-care patients.

Characteristics of after-care patients.


Site of corticotropin-releasing factor (CRF) releasing elements: effect of lesions on ACTH release and adenohypophysial blood flow.


The effect of hypertonic solution on the wet weight and contractions of rat uterus and vas deferens. The role of propagated activity in the responses to agonist drugs was studied for the rat uterus and vas deferens. Hypertonic solutions were used to inhibit propagation of activity by shrinking cells. Tissue weight was used to indicate cell volume. Hypertonic solutions after 10 min caused weight loss and reduced the size of contractions in response to submaximal doses of drugs, to KCl, and to external electrical stimulation. Contractions in response to KCl and drugs were diminished to a similar degree in the vas deferens, but in the uterus, drug contractions were depressed much more. Prolonged action of hypertonic solution also differed for the two tissues. In the uterus, weight changes correlated with changes in size of the drug-induced contractions. Uterine contractions reduced in hypertonic solution could be increased by using supramaximal doses of drug. When stimulation was applied to one end of the uterus in a three compartment bath, propagation of spontaneous drug- and KCl-induced contraction occurred, but it was prevented by placing hypertonic solution in the center compartment. An increase of the KCl to 44 mM in the hypertonic solution restored propagation. These experiments yielded no evidence of propagated responses in the rat vas deferens. It was concluded that propagated activity plays a role in drug-induced contractions in the rat uterus but not in the rat vas deferens. Hyperpolarization of shrunken cells might be involved in inhibition of propagation by hypertonic solutions.


Inhibitory miniature potentials in the stretch receptor neurons of crayfish. Intracellular microelectrodes inserted into the soma of crayfish stretch receptor neurons record frequent fluctuations of the membrane potential. Time course, amplitude, and interval distribution indicate that they are miniature potentials. At the average resting potential the polarity of the miniature potentials depends on the anion used in the microelectrode: KCl electrodes record depolarizing, K citrate or K(2)SO(4) electrodes, hyperpolarizing miniature potentials. The inhibitory postsynaptic potentials (i.p.s.p.'s) show a similar polarity change. The reversal potentials of i.p.s.p.'s and miniature potentials are equal and within 10 mv of the resting potential, more negative with K citrate (or K(2)SO(4)), less negative with KCl electrodes. Reversal can be accomplished by changing the membrane potential by stretching or by current passing. Injection of Cl(-) into the soma or replacement of external Cl by propionate results in an abrupt increase of the amplitude of the miniature potentials lasting for several minutes. The miniature potentials like the i.p.s.p.'s are reversibly abolished by the application of picrotoxin and gamma-aminobutyric acid. They are not affected by tetrodotoxin, nor by acetylocholine, eserine, or atropine. It is concluded that the miniature potentials represent a spontaneous quantal release of transmitter substance from inhibitory nerve terminals, and that the transmitter substance predominantly increases the Cl(-) permeability of the postsynaptic membrane. The effect of the spontaneously released transmitter on the behavior of the receptor neuron is considerable. The membrane conductance is increased by up to 36% and the excitability is correspondingly depressed.


A heart transplantation. 6. Pathological aspects.


Rehabilitation of the cancer patient.

Rehabilitation of the cancer patient.


Primary liver carcinoma in Brisbane.

Primary liver carcinoma in Brisbane.


A heart transplantation. 7. Microbiology and antibiotic chemotherapy.


Viruses infecting cattle and their role as endogenous contaminants of cell cultures.


Satellite viruses, helper viruses, and synergistic action.


Primary herpes infection of right second finger. Report of a case.


A sensitive electrocardiographic sign in myocarditis associated with viral infection.


Familial pituitary dwarfism associated with an abnormal sella turcica.


A phosphatidase produced by Sclerotium rolfsii.


Enhancement of responses to chemical carcinogens by nononcogenic viruses and antimetabolites.


Tumor-promoting agents in two-stage carcinogenesis.


Bioassays with inbred mice: their relevance for the random-bred animal.


General neurophysiology (bioelectrical aspects).


Hormones in social amoebae and mammals.

Hormones in social amoebae and mammals.


Electroencephalography.

Electroencephalography.


Adenyl cyclase activity in rat pineal gland: effects of chronic denervation and norepinephrine. Adenyl cyclase activity in the pineal gland of rats was determined by measuring the rate of formation of radioactive cyclic 3',5'-adenosine monophosphate from (14)C-labeled adenosine triphosphate. Norepinephrine added in vitro to pineal homogenates enhanced this activity, while denervation of the pineal gland by superior cervical ganglionectomy did not significantly reduce it. The enzyme in these denervated glands was more responsive to the stimulatory effects of norepinephrine.


Diphtheria toxin subunit active in vitro. Exposure of diphtheria toxin to dithiothreitol (and similar thiols) resulted in a subunit which was active in catalyzing the adenosine diphosphateribosylation of mammalian aminoacyl-transferase II in the presence of nicotinamide adenine dinucleotide. At the same time there was a marked increase in total ADP-ribosylation activity. A molecule which was apparently identical to the derived subunit in size and activity was detected in partially purified preparations of toxin.


Alteration of a cat small intestine mechanoreceptor response by ionizing radiation. NRDL-TR-68-99.


Immune drug-induced haemolytic anemias.

Immune drug-induced haemolytic anemias.


Use of enzyme hydrolysis in the rapid analysis of blood estrogens.


Deoxyribonucleotide synthesis and the role of vitamin B12 in erythropoiesis.


Viruses in carcinogenesis.

Viruses in carcinogenesis.


An equine genital infection resembling coital exanthema associated with a virus.


Hydrogen bonding of vitamin D and egg lecithin in the solid state. A possible molecular role for vitamin D in calcium binding by membranes.


Distribution of (Na+-K+)-stimulated ATPase activity in rat intestinal mucosa.


Thallium activation of K+-activated phosphatases from beef brain.


Rhinoviruses of bovine origin.

Rhinoviruses of bovine origin.


The effect of cortisone, ACTH, and TSH on mast cell count and on mast cell nonspecific esterase.


The antidiuretic activity of the hypothalamus, hypophysis and blood plasma under various states of water metabolism and adrenergic transmission.


Amputation neuroma. A case history.

Amputation neuroma. A case history.


Human skin collagenase: localization and distribution in normal human skin.


[Functional adrenocortical activity and developmental stages of laryngeal carcinoma].


Multiple glomus tumors. An ultrastructural study.


Human skin collagenase: relationship to the pathogenesis of epidermolysis bullosa dystrophica.


Organization of the histidine-3 region of Neurospora.


Deep electrical burn complicated by anuria and pseudomonas septicaemia.


Complex synaptic configurations in planarian brain. Complex synaptic configurations which appear to have especial evolutionary and functional significance are shown in the neuropil of the brain of the planarian Dugesia dorotocephala. Some of the endings in these synaptic attachments contain dense core vesicles, suggesting that nonadrenaline or serotonin or both are neurotransmitters at a more primitive phyletic level than reported hitherto. The spatial proximity and connectivity of the synapses suggest modes of action permitting greater functional complexity to the planarian brain than previously supposed. Closely adjacent cellular processes which contain polysomal ribosomes, unusual in the neuropil, suggest synaptic transmission-protein synthesis coupling and a possible role in memory.


Observations on current antibiotic usage in the surgical patient.


Neoplastic transformation in vitro of hamster lens epithelium by simian virus 40. Hamster lens epithelium infected with simian virus 40 underwent transformation in vitro and produced tumors when injected into homologous hosts. Undisturbed lens epithelium in man and experimental animals has not been observed to undergo neoplastic change. The virus-induced tumors contained undifferentiated cells that were either polygonal or spindle-shaped. Their origin from lens epithelium seems certain since it is possible to isolate this unique structure free of connective tissue and blood vessels.


The biochemical basis of adrenal surgery.


Lipids in cerebrospinal fluid. XII. In multiple sclerosis and retrobulbar neuritis.


Immunocytochemistry of acidophil granules of human pituitary.


Electromyography in spinal cord injury.

Electromyography in spinal cord injury.


Natural and "induced" sleep.

Natural and "induced" sleep.


Anterior interosseous nerve palsy.

Anterior interosseous nerve palsy.


The metabolism of pregnenolone and progesterone by cobra adrenal tissue in vitro and the effect of ACTH on product yield-time curves.


Suppression by dexamethasone of interrenal activity in adult sockeye salmon (Oncorhynchus nerka).


The effect of flavin isomers and analogues upon the color of bacterial bioluminescence.


Kinetic and regulatory properties of heart muscle pyruvate dehydrogenase.


The bacterial oxidation of vitamin B6. VII. Purification, properties, and mechanism of action of an oxygenase which cleaves the 3-hydroxypyridine ring.


The bacterial oxidation of vitamin B6. VI. Pyridoxal dehydrogenase and 4-pyridoxolactonase.


Electron paramagnetic resonance and circular dichroism studies on milk xanthine oxidase.


Transport of amino acids by confluent and nonconfluent 3T3 and polyoma virus-transformed 3T3 cells growing on glass cover slips.


The role of molybdenum in xanthine oxidase and related enzymes. Reactivity with cyanide, arsenite, and methanol.


New observations on the substrate specificity of cathepsin C (dipeptidyl aminopeptidase I). Including the degradation of beta-corticotropin and other peptide hormones.


Periodate oxidation of pseudouridine 5'-phosphate and spectral characterization of the products.


The foetal sheep: morphogenesis of the nervous system and histochemical aspects of myelination.


Use of the fluorescent antibody method in the diagnosis of M. parainfluenzae. I. Proliferation of M. parainfluenzae types 1, 2 and 3 in monkey kidney tissue cultures.


Antimicrobial agents. Selection and use.


Course of air-borne infection caused by B virus (herpesvirus simiae). I. Lethal inhalation dose of B virus for rabbits.


Lipid composition of human, bovine and sheep pineal glands.


Tetanus toxin and amino acid levels in cat spinal cord.


Simplified premedication for brain scans and other radioisotope tests.


The effect of substrates and inhibitors on the rate of respiration of the infective larvae of Strongyloides ratti Sandground, 1925.


Alpha-keto acid dehydrogenase complexes. X. Regulation of the activity of the pyruvate dehydrogenase complex from beef kidney mitochondria by phosphorylation and dephosphorylation. This paper reports the discovery that the activity of the multienzyme pyruvate dehydrogenase complex from beef kidney mitochondria is regulated by a phosphorylation-dephosphorylation reaction sequence. The site of this regulation is the pyruvate dehydrogenase component of the complex. Phosphorylation and concomitant inactivation of pyruvate dehydrogenase are catalyzed by an ATP-specific kinase (i.e., a pyruvate dehydrogenase kinase), and dephosphorylation and concomitant reactivation are catalyzed by a phosphatase (i.e., a pyruvate dehydrogenase phosphatase). The kinase and the phosphatase appear to be regulatory subunits of the pyruvate dehydrogenase complex.


Incorporation of branched-chain fatty acids into myxoviruses. The incorporation of free fatty acids into myxoviruses was shown, using branched-chain fatty acids as molecular markers. The presence of isostearic or phytanic acid was detected by gas-liquid chromatography in the phospholipid fraction of the A(0)/PR8/34 strain of influenza virus. Uptake of free fatty acids into the virus varied from 8 to 11 per cent and was accompanied by a shift in the fatty acid profile. Infected allantoic fluids from eggs treated with branched-chain acids possessed higher hemagglutinin activity when compared to fluids infected under normal conditions. Attempts to detect branched-chain acids in Sendai virus were unsuccessful. Shifts in acyl chain composition persisted after three passages of modified viruses in the absence of branched-chain acids. Force-area curves at an air-water interface revealed the cross-sectional area of branched-chain acids to be greater than their straight-chain homologs. It is suggested that hydrophobic interactions can alter the configuration of envelope proteins. Such changes may have an important role in the selection of fragments of influenza viral genome and can conceivably alter viral genotype.


Donor-acceptor interactions of nitrogen. The nitrogen atoms of organic molecules readily enter into donor-acceptor interactions, giving off an electron from their lone pair. Under favorable conditions the acceptor can form free radicals. S and O atoms behave likewise but less intensely.


Strain MC29 avian leukosis virus release by chick embryo cells infected with the agent.


Mammary tumor virus activity in mammary tissues of hormone-stimulated BALB-cfC3H-Crgl mice.


A technic for the preparation of isolated rat adrenal cells.


Prevention of drop in adrenocortical activity in the 7-day-old rat by pretreatment with ACTH.


Absence of infectious virus from a line of SV40-transformed human liver cells.


Hypoglycemia and its relationship to histamine sensitization in mice.


Enhancing effect of the murine sarcoma virus (MSV) on the replication of the mouse hepatitis virus (MHV) in vitro.


Enhancement of vesicular stomatitis virus following adsorption with poly-L-ornithine.


Abortive infection of human cell cultures by a canine adenovirus.


Fate of labeled polyoma virus DNA in cell cultures.


Nucleoside phosphatase activity in atrial and ventricular myocardium of the rat: a light and electron microscopic study.


Oysters and human viruses: effect of seawater turbidity on poliovirus uptake and elimination.


Genetic characteristics of poliovirus strains isolated in Poland.


Association between autoregulation and pressure dependency of renal vascular responsiveness in dogs.


Treatment of median sternotomy infection by mediastinal irrigation with an antibiotic solution.


Cutaneous venoconstrictor response to local cooling in the dog. Unexplained by inhibition of neuronal re-uptake of norepinephrine.


The biochemical mechanism of selective heat sensitivity of cancer cells. I. Studies on cellular respiration.


Antigenic relationships among the coronaviruses of man and between human and animal coronaviruses.


Density of BAI strain A avian leukosis virus-antibody complexes relative to antigen-antibody ratio.


Metaphase figures of rat chromosomes incorporated into mouse cells.


Adenoid cystic carcinoma of the uterine cervix. Report of a case and review of 4 cases.


[Role of the mediodorsal thalamic nucleus in temporal lobe seizures--an experimental study].


The use of plasma cortisol estimations in the management of colitis.


Moss-ill--a possible hypocalcaemia of hill ewes.


Beta-adrenergic receptors in human platelets.


Effect of ozone. On pyridine nucleotide reduction and phosphorylation of Euglena gracilis.


Phage 4 and polymyxin B sensitivity of rough strains of classicial V. Cholerae.


Old people leaving hospital. A follow-up of 200 discharged geriatric patients.


Cytopathic enteroviruses in Delhi area. I. From cases of diarrhoea and healthy controls.


Immunochemical studies of an organ-specific thermostable antigen of bovine brain.


What's new with bovine respiratory problems.


Relationship of viruses to reproductive problems.


Assessment of cerebral lesions by rapid sequential scintiphotography.


Pressure gradients and lymph flow in the canine thoracic duct.


The absence of central sympathetic stimulation by low doses of angiotensin in the cat.


Calcium pyrophosphate dihydrate crystal deposition disease.


Chronic idiopathic neutropenia. Treatment with corticosteroids and mercaptopurine.


Nonepidermoid cancer of the gum.

Nonepidermoid cancer of the gum.


Comparison of total and prostatic fraction serum acid phosphatase levels in patients with differentiated and undifferentiated prostatic carcinoma.


Nonfunctional, nonchromaffin paragangliomas of the retroperitoneum.


Extra-uterine Müllerian tumors. A review of the literature and the report of a case.


The mode of action of an intrauterine contraceptive device.


Electrophysiology of the lizard cochlea.


Herpes simplex virus infection and depressive illness.


Preparation of fat cell-"free" rat mammary gland.


Organ specific effect of thioacetamide on liver glycogen.


The relationship of intrarenal artery and renal intrapelvic pressure.


The significance of the nerve cell.

The significance of the nerve cell.


The discharged mentally ill patient: aftercare and maintenance in the community.


LDH isozyme activities in subcellular fractions of liver and hepatoma.


The mononucleotides.

The mononucleotides.


Experience with hepatic resection in childhood.


Functioning beta slet cell tumors. Preoperative tests and surgical approach.


Characteristics of nonbacteremic Pseudomonas pneumonia.


Reversible respiratory paralysis associated with polymyxin therapy.


The neuropathy of Sjögren's syndrome. Trigeminal nerve involvement.


Treatment of breast cancer with medroxyprogesterone acetate. The prolonged administration of large doses of medroxyprogesterone acetate (MPA) in the treatment of patients with disseminated breast cancer was studied.  The patients consisted of 17 postmenopausal women previously treated with 1 or more hormones (group I), 17 women (oophorectomized or postnatural menopause) who had received MPA prior to the study (group II), and 2 males with breast carcinoma  (group III).  Patients received MPA intramuscularly (as Depo-Provera, 100 mg 3 times weekly, 34 patients) or orally (as Provera, 100 mg daily, 2 patients), in all except 4 cases for a minimum of 5 weeks.  Patients were classified as showing objective tumor regression or as being treatment failures.  4 patients, because of treatment for neurological complications, were judged not evaluable.  5 group-I patients and 2 group-II patients (both greater than 10 years since menopause) experienced objective tumor regression.  All 7 patients received the MPA parenterally.  By the end of the study mean duration of the objective remissions was 12 months.  3 of the 7 patients continued in remission at writing, 2 had succumbed to the disease, and 2 were receiving other agents.  Site of involvement in these patients was predominantly osseous (3), visceral (2), or soft tissue (2).  Mean age of the 7 responders was 69.  The mean "free interval" from initial therapy to clinical appearance of distal metastases was 44 months in the responders.  All 5 patients from group I had benefited from previous hormonal alterations.  Of 10 patients receiving estrogens in combination with MPA immediately after the course of MPA alone, 5 demonstrated objective tumor regression, 1 who had responded to MPA alone.  The best response in this group occurred in a 61-year old man.  Most patients had no untoward effects with MPA administration.  A fairly extensive discussion of the literature on MPA is included.


Chronic laxative-induced hyperaldosteronism and hypokalemia simulating Bartter's syndrome.


NIH clinical staff conference. Burkitt's tumor.


Metabolism of arterial tissue and its relation to atherosclerosis.


Vascular enzyme activities and susceptibility of arteries to atherosclerosis.


Histochemistry of the development of atherosclerosis.


Enzymic omega-oxidation: stoichiometry of the omega-oxidation of fatty acids.


Great-toe extensor reflexes in the diagnosis of lumbar disc disorder. The tendon reflexes obtainable from the extensors of the great toe require practice to elicit, but have been found particularly valuable in the assessment of lesions affecting the fifth lumbar nerve root, especially compression radiculopathy from disc prolapse.


The control of lipid biosynthesis in liver particles.


Cytopathic enteroviruses in Delhi area. II. From cases of febrile illnesses with neurological involvements.


Susceptibility of Bonnet monkey (M. radiata) kidney cell cultures to human enteroviruses.


Japanese encephalitis studies in Vellore, South India. V. Experimental infection and transmission.


Pyridine nucleotides: nicotinamide adenine dinucleotide and the cholinergic mechanisms.


Binding of ADP-ribose polymer with histone.


Isolation and base composition of sites on lambda phage DNA which bind with RNA polymerase of Escherichia coli.


Glycine metabolism by rat liver mitochondria. II. Methylene tetrahydrofolate as the direct on carbon donor in the reaction of glycine synthesis.


Glycine metabolism by rat liver mitochondria. 3. The glycine cleavage and the exchange of carboxyl carbon of glycine with bicarbonate.


Effect of angiotensin-II-amide on renal function in the clawed toad, Xenopus laevis Daudin.


The effect of aldosterone and angiotensin on the intestinal transport of sodium and water in the rat.


Studies on the influence of renal nerves on the regulation of aldosterone secretion.


Crisis in myasthenia gravis.

Crisis in myasthenia gravis.


Necrotizing encephalomyelopathy. Report of a case with relapsing polyneuropathy and hyperalaninemia and with manifestations resembling Friedreich's ataxia.


A prospective study of the role of cytomegalovirus in post-transfusion mononucleosis.


Viruses in circulating lymphocytes.

Viruses in circulating lymphocytes.


Abnormal fibrinogen synthesis questioned.


Radiation-induced free radicals in frozen nitric acid solutions.


Free radicals in gamma-irradiated 2-deoxy-D-ribose: electron spin resonance of single crystals and polycrystalline material.


Electron spin resonance of gamma-irradiated single crystals of 9-methyladenine.


Effects of head irradiation on paradoxical sleep (EEG afterreaction) in the rabbit.


Extralymphatic extravasation of ethiodol during lymphography.


How do viruses cause cancer? New Horizons for Radiologists Lecture.


Histochemical study on plasma cells.

Histochemical study on plasma cells.


The effect of spermine and oxidized spermine on the enzymic degradation of DNA.


Ribonucleic acid-degrading enzymes associated with purified ribosomes from Neurospora crassa mycelia.


Incidence of cytomegalic inclusion disease.


Interpretation of the multiple forms of pig heart lipoamide dehydrogenases.


Adenosine diphosphatase in the electron transfer particle.


Structural proteins of vesicular stomatitis viruses. Three major and three minor structural proteins were identified by polyacrylamide gel electrophoresis of purified infectious virions of the Indiana serotype of vesicular stomatitis (VS) virus disrupted with acetic acid, 0.5 m urea, sodium dodecyl sulfate (SDS), and 2-mercaptoethanol. Molecular weights of the six virion proteins were estimated by comparative electrophoretic migration of known marker proteins in the presence of SDS. The following values were obtained: major proteins P6 congruent with 34,500, P5 congruent with 59,500, and P4 congruent with 81,500; minor proteins P3 congruent with 140,000, P2 congruent with 186,000, and P1 congruent with 275,000. P1 did not disaggregate in 8 m urea, but P2 and P3 did. The possibility that P1 is an uncleaved large polypeptide chain could not be ruled out. Six identical protein components were dissociated from Indiana VS virions grown in chick and mouse cells; no cellular proteins could be detected in purified virions. Of six proteins identified in virions of the New Jersey serotype, only the smallest protein (P6) could be distinguished from any of the six proteins of the Indiana serotype on the basis of migration in SDS gels. The defective T particles of Indiana VS virus contained the same six proteins in essentially the same proportions as those of the infectious B virions. Only P6 and P5 could be cleanly separated by preparative gel electrophoresis.


In vitro synthesis of poliovirus ribonucleic acid: role of the replicative intermediate. Poliovirus ribonucleic acid (RNA) polymerase crude extracts could be stored frozen in liquid nitrogen without loss of activity or specificity. The major in vitro product of these extracts was viral single-stranded RNA. However, after short periods of incubation with radioactive nucleoside triphosphates, most of the incorporated label was found in replicative intermediate. When excess unlabeled nucleoside triphosphate was added, the label was displaced from the replicative intermediate and accumulated as viral RNA. It is concluded from this experiment that the replicative intermediate is the precursor to viral RNA. In addition, some of the label was chased into double-stranded RNA. The implications of this finding are discussed.


Differential inhibition of attachment and eclipse activities of HeLa cells for enteroviruses. Receptor activities of HeLa cells were evaluated for ability to both attach and eclipse enteroviruses after exposure of cells to acid or heat. A modified procedure of acid (pH 1.5) elution of cell-associated virus, as compared with other procedures, provided a general method for the optimal recovery of receptor-bound enteroviruses. With this procedure, eclipse of virus operationally was considered to be that amount of virus infectivity which was determined initially to be cell-associated and which was not dissociable from the cells. HeLa cells killed by heating at 56 C for 30 min could not attach or eclipse poliovirus T1, but they attached and eclipsed coxsackieviruses B1 and B3, and they attached echovirus 6 but did not eclipse it. HeLa cells treated at pH 2.5 for 10 min at 2 C could not attach or eclipse poliovirus T1, but they attached coxsackieviruses B1 and B3 and echovirus 6, although these viruses were not eclipsed. These results showed that, within the operational definition of virus eclipse, the eclipse activity of HeLa cells for some viruses can be irreversibly inactivated without impairing the activity of the receptors for attaching these viruses. The data provided additional evidence that HeLa cells possess specific receptors for the different enteroviruses.


Proteins of vesicular stomatitis virus. I. Polyacrylamide gel analysis of viral antigens. Infection of L cells with vesicular stomatitis virus results in the release, into the cell-free fluid, of four antigenic components separable by rate zonal centrifugation on sucrose gradients. The largest antigens are the infectious (B) particle and a shorter noninfectious, autointerfering (T) particle. The two small antigens are characterized by sedimentation coefficients of approximately 20S and 6S. Treatment of purified B or T particles with sodium deoxycholate results in the release from the particle of a nucleoprotein core which can be purified on sucrose gradient and which has a sedimentation coefficient characteristic of the virus from which it arose. Utilizing purified antigens labeled with (14)C-amino acids during growth, we examined the protein constituents of each antigen by acrylamide-gel electrophoresis. The proteins of B and T particles are identical, each containing one minor (virus protein 1) and three major (virus proteins 2, 3, and 4) proteins, numbered in order of increasing mobility. Virus protein 3 originates from the nucleoprotein core, whereas proteins 2 and 4 come from the coat. The origin of virus protein 1 is not known. The 20S antigen contains a single protein equivalent to virus protein 3, whereas the 6S antigen shows a single protein which is similar to, but probably distinct from, virus protein 2.


Immunochemical studies of phospholipids. V. Rabbit antiserum against cardiolipin antigen and its possible application to histochemistry.


Response to 4-dimethylaminoazobenzene (DAB) of mammalian cells in culture.


Some observations on the behavior of Culex tritaeniorhynchus summorosus, the prinicipal vector of Japanese encephalitis in Japan.


In vitro activity of N',N'-anhydrobis (beta-hydroxyethyl) biguanide hydrochloride (ABOB) against parainfluenza viruses.


Solid-cystic hidradenoma: an enzyme histochemical, biochemical, and electron microscopic study.


Nucleic acids and protein synthesis in cancer cell mitochondria. II. Amino acid incorporation into proteins of rat liver and hepatoma cell mitochondria.


A method of column chromatographic isolation of major phospholipid components on Escherichia coli.


A comparison between methods of calculating skeletal age (Greulich-Pyle).


Cavitary bronchiolar carcinoma. A case report.


A review of the action of isoniazid.

A review of the action of isoniazid.


Glomus tumour in an African.

Glomus tumour in an African.


Cross-resistance relationships between capreomycin, kanamycin, and viomycin resistances in tubecle bacilli from paients.


Theprostaglandins: biologically active lipids with implications in circulatory physiology.


Physiological and pathological variations of plasma angiotensin II in man. Correlation with renin activity and sodium balance.


Quantitative analysis of the pathophysiology of hypertension.


The role of vessel wall reactivity in experimental hypertension.


Phosphorylethanolamine and hypophosphatasia.


Neuromuscular synapses in the cockroach extensor tibiae muscle.


Free radical destruction by gamma-irradiation at 77 degrees K.


Antigenic relationship among strains of infectious canine hepatitis virus.


Characteristics in tissue culture of infectious canine hepatitis virus-induced hamster tumor cells.


Oncogenicity of infectious canine hepatitis virus in hamsters.


Effects of angiotensin and other peptides on ciliary movement of frog esophagus.


Amoebiasis: its meaning and diagnosis.

Amoebiasis: its meaning and diagnosis.


The specificity and mechanism of the Kober reaction.


Synthesis of 25-hydroxycholecalciferol, the biologically effective metabolite of vitamin D.


Ethanol induced increase of the ratio between hydroxy- and ketosteroids in human pregnancy plasma.


Markers differentiating type 3 parainfluenza virus grown at 25 and 37 C. Serial passage of the 64-2389 strain of type 3 parainfluenza virus in cercopithecus monkey kidney tissue cultures at low temperatures resulted in the selection of a variant which had a higher efficiency of plaque formation at 25 C than the parent line grown at 37 C. The cold variant, unlike the parent strain, plaqued readily at 25 C, and at 37 C it produced significantly larger plaques. Virus titers of the cold variant in hamster lungs were significantly lower and this was probably caused by the stimulation of interferon by the cold variant during the early phase of the infection. The cold variant, like the virus grown at 37 C, also induced the synthesis of interferon late in the infection. Hamsters responded to the intranasal inoculation of each virus line by the development of hemagglutinating-inhibiting antibodies in the sera.


Concentration and purification of herpesviruses (simplex, cytomegalo, and EB) in a zonal ultracentrifuge. By using the BXIV zonal ultracentrifuge rotor, it was possible to efficiently concentrate and partially purify members of the herpesvirus group by pelleting the virus onto a double sucrose cushion.


Microneutralization test for determination of rhinovirus and coxsackievirus A antibody in human diploid cells. A method for determination of serum-neutralizing antibody titers to rhinovirus type 16 and coxsackievirus type A-4 in human diploid cells (WI-38) grown in Microtiter plates is described. A good correlation was observed when comparing neutralization tests in WI-38 and other cells. The WI-38 Microtiter method is relatively simple and economical. It is suitable for those laboratories which are required to conduct large-scale serological evaluation of antibodies for rhinoviruses and coxsackie A viruses. This system also can be used for cytomegalovirus and varicella-zoster virus, which grow relatively slowly.


Study of delayed hypersensitivity to myxoviruses induced by vaccines. The delayed hypersensitivity response of guinea pigs to Bacillus Calmette-Gúerin (BCG) and myxovirus vaccines was investigated by use of the techniques of skin testing and inhibition of macrophage migration. A serum antibody response to the injected vaccines was readily demonstrable. Parainfluenza type 2 virus consistently failed to induce a delayed hypersensitivity state in 15 animals, even with the use of a virus adjuvant emulsion. Respiratory syncytial virus occupied an intermediate position in that positive delayed type skin tests of an erythematous nature were elicited following inoculation, but only two of seven guinea pigs yielded a positive migration inhibition test. In mumps-inoculated animals, skin testing gave rise to erythematous delayed skin reactions which varied from 0 to 20 mm in size. Migration inhibition could be demonstrated in 7 of 21 animals. In almost all guinea pigs inoculated with BCG, large, indurated, erythematous skin reactions were elicited, and inhibition of macrophage migration was readily demonstrated. The degree of skin reactivity was positively correlated with inhibition of macrophage migration. If the skin reaction to a specific antigen exceeded 9 mm of erythema, that antigen also inhibited macrophage migration. Skin testing proved to be the most sensitive indicator of viral hypersensitivity. Migration inhibition was demonstrated only when a greater than 8-mm skin reaction was evoked. This cellular hypersensitivity appeared to be a qualitative phenomenon, the expression of which could be heightened by the use of adjuvant. The applicability and sensitivity of the migration inhibition technique is considered relative to its use for in vitro monitoring of effects of viral vaccine inoculations.


Minicultures of mammalian cells in a new plastic plate. A new disposable micro tissue culture plate was developed and tested for use in virological procedures. Miniature mammalian cell cultures (minicultures) were grown in these plates. Each plate contained 96 circular cultures in flat wells (7 mm in diameter). Replicate titrations of a number of viruses were performed in various tissues. Excellent reproducibility was demonstrated. Mean infectivity titers determined by miniculture methods were generally within 0.6 log(10)/ml of macro tube titrations. Standard tissue culture assay techniques such as hemadsorption, interference titration, and microneutralization were easily carried out with this method and were very reproducible. Development of this noncytotoxic disposable micro tissue culture plate now permits the routine performance of rapid, reliable, and reproducible tissue culture tests at a very significant reduction in cost and labor.


Roles of collagenases and other proteolytic enzymes in the dispersal of animal tissues.


Rabbit intestinal microsomal inorganic pyrophosphate-glucose phosphotransferase: sugar inhibitor specificity.


Purification and properties of the enzyme ATP-sulfurylase and its relation to vitamin A.


Intramitochondrial localization of 5-aminolaevulinate synthase induced in rat liver with allylisopropylacetamide.


UDP-D-glucuronate 4-epimerase in blue-green algae.


The role of the reticular activating system in the regulation of ACTH secretion.


Interconvertible forms of muscle phosphorylase phosphatase.


Differential effects of sodium butyrate and physostigmine upon the activities of para-sleep in acute brain stem preparations.


2',3'-cyclic nucleotide 3'-phosphohydrolase in the brain of the "Jimpy" mouse, a mutant with deficient myelination.


Developmental patterns in the central nervous system of birds. V. Cerebral electrical activity, forebrain function and behavior in the chick at the time of hatching.


Inhibition of transmission in the recurrent inhibitory pathway to motoneurones.


A study on the effect of Flagyl on Entamoeba histolytica.


Enzymes of protein and phosphate catabolism in rat bone. I. Enzyme properties in normal rats.


Enzymes of protein and phosphate catabolism in rat bone. II. Changes in vitamin D deficiency.


The part played by the extracellular components (collagen and albumin) in the decrease in reactivity of adipose tissue in old rats.


Changes in the structure of collagen in the direction of ageing in relation to decreased lipomobilization in rats.


Comparative studies on the sensitivity of some of the methods of complement fixation test commonly used for typing of foot and mouth disease virus.


Spectrophotometric titration of ferredoxins and Chromatium high potential iron protein with sodium dithionite.


Metabolic interlock. Regulatory interactions exerted between biochemical pathways.


Structure of cell wall lipopolysaccharide from Salmonella typhimurium. I. Linkage between o side chains and R core.


Lactate dehydrogenase of lobster (Homarus americanus) tail muscle. II. Kinetics and regulatory properties.


Regulation and mechanism of phosphoribosylpyrophosphate synthetase. I. Purification and properties of the enzyme from Salmonella typhimurium.


Studies of the electron transport chain of extremely halophilic bacteria. II. Salt dependence of reduced diphosphopyridine nucleotide oxidase.


Lactate dehydrogenase of lobster (Homarus americanus) tail muscle. I. Physical and chemical properties.


Enzymatic synthesis of deoxyribonucleic acid. XXVII. Chemical modifications of deoxyribonucleic acid polymerase.


Enzymatic synthesis of deoxyribonucleic acid. 28. The pyrophosphate exchange and pyrophosphorolysis reactions of deoxyribonucleic acid polymerase.


Electron spin-echo studies of relaxation processes in high-spin ferrimyoglobin.


The properties of glycogen synthetase and regulation of glycogen biosynthesis in rat brain.


The separation of herpes virus-specific antigens by polyacrylamide gel electrophoresis.


The binding of DNA polymerase to polyoma DNA.


The preparation of 'monoprecipitin' antisera to herpes virus specific antigens.


Biological markers for differentiation of herpes-virus strains of oral and genital origin.


Comparison of the antiviral activity in vitro of some non-steroidal anti-inflammatory drugs.


Specific tumour antigen induced by chick embryo lethal orphan (CELO) virus.


Immunochemical characterization of plaque mutants of simian virus 40. Analysis of large and small plaque mutants of simian virus 40 using antisera prepared against each has revealed quantitative and possibly qualitative antigenic differences for each plaque type. A sensitive micro radioisotope precipitation test permitted evaluation of immunochemical similarities and differences of capsid antigens by inhibition of precipitation.


Simian virus 40 deoxyribonucleic acid replication. I. Effect of cycloheximide on the replication of SV40 deoxyribonucleic acid in monkey kidney cells and in heterokaryons of SV40-transformed and susceptible cells. Infectious deoxyribonucleic acid (DNA) was extracted from green monkey kidney (CV-1) cultures at various times after the cultures were infected with simian virus 40 (SV40) at input multiplicities of 0.01 and 0.1 plaque-forming unit (PFU) per cell. A pronounced decrease in infectious DNA was observed from 3 to 16 hr after virus infection, suggesting that structurally altered intracellular forms may have been generated early in infection. Evidence is also presented that SV40 DNA synthesis requires concurrent protein synthesis. DNA replication was studied in the presence and absence of cycloheximide in: (i) SV40-infected and uninfected cultures of CV-1 cells; (ii) cultures synchronized with 1-beta-d-arabinofuranosylcytosine (ara-C) for 24 to 30 hr prior to the addition of cycloheximide; and (iii) in heterokaryons of SV40-transformed hamster and susceptible monkey kidney cells. DNA synthesis was determined by pulse-labeling the cultures with (3)H-thymidine at various times from 24 to 46 hr after infection. In addition, the total infectious SV40 DNA was measured. Addition of cycloheximide, even after early proteins had been induced, grossly inhibited both SV40 and cellular DNA syntheses. The activities of thymidine kinase, DNA polymerase, deoxycytidylate deaminase, and thymidylate kinase were measured; these enzyme activities remained high for at least 9 hr in the presence of cycloheximide. SV40 DNA prelabeled with (3)H-thymidine before the addition of cycloheximide was also relatively stable during the time required for cycloheximide to inhibit further DNA replication.


Contrasting effects of polycations on plaquing efficiency of encephalomyocarditis virus variants. Polycation treatment of L cell monolayers affected plaquing efficiency of both the r(+) and r variants of the encephalomyocarditis virus. Plaque formation by r(+) variant was decreased markedly by three structurally different types of synthetic basic polymers, diethylaminoethyl dextran, hexadimethrene (polybrene), and basic polyamino acids. In contrast, these same substances increased substantially the number of plaques formed by the r variant. The effect on the two variants was observed when polycations were applied to the cells before or simultaneously with the introduction of virus. The molar concentration and size of the polymer proved important. Thus, basic polyamino acids of low molecular weight were significantly more inhibitory for the r(+) variant than were those of high molecular weight. On the other hand, plaquing efficiency of the r variant was increased by relatively large polyamino acids, but not by polymers of small size. Basic polyamino acids inhibited r(+) plaque formation to a greater degree at low than at high pH values. However, plaquing efficiency of the r variant in polycation-treated cultures was not affected by changes in pH. Basic polymers appear to bind to cell membranes and affect either attachment or uptake of the viruses. The evidence suggests that the substances influence by different mechanisms the interaction of the r(+) and r variants with cells.


Formation of viral ribonucleic acid and virus in cells that are permissive or nonpermissive for murine encephalomyelitis virus (GDVII). GDVII virus growth in BHK-21 cells, a permissive host for the virus, resembled productive infections with other picornaviruses. Virus yields ranged from 100 to 600 plaque-forming units (PFU)/cell. Virus replication in HeLa cells, a nonpermissive host for GDVII virus, was characterized by virus yields of only 0.1 to 5 PFU/cell. Similar low yields of virus have been obtained from HeLa cells at all multiplicities of input up to 6,000 per cell. The progeny particles from HeLa cells were, like the infecting particles, restricted in the HeLa cell host. Despite the great difference in final yields of virus from BHK-21 and HeLa cells, the times when maximal yields were reached were similar. GDVII virus stock grown in BHK-21 cells was designated HeLa(-). A variant of GDVII virus which is capable of extensive growth in HeLa cells was obtained. This variant, designated HeLa(+) GDVII virus, was passaged serially in HeLa cells. Virus yields of 50 to 150 infective virus particles per cell were obtained from infection of HeLa cells with HeLa(+) GDVII virus. The major species of HeLa(+) virus-specific ribonucleic acid (RNA) produced was single stranded and sedimented with an S value of 35S. The rate of accumulation of HeLa(+) virus-specific RNA in HeLa cell cultures was about four times that of HeLa(-) RNA. The amount of virus-specific HeLa(+) RNA formed in HeLa cells was several-fold greater than that of HeLa(-) RNA. With HeLa(-) parent GDVII virus undergoing productive replication in BHK-21 cells or abortive replication in HeLa cells, the major species of virus-specific RNA produced was single stranded and sedimented with an approximate S value of 35S. The amount of HeLa(-) virus-specific RNA extracted from BHK-21 cells was several-fold greater than the amount obtained from HeLa cells.


Replication of human adenovirus in green monkey kidney cells transformed by adeno 7-SV40 hybrid virus.


Titration and extensive serial passages of Yaba virus in vitro.


Immunochemical and biochemical studies of fungi. 13. On the galactomannans isolated from mycelia and culture filtrates of several filamentous fungi.


Electron microscopic observations of the cells infected with varicella-zoster virus.


Oral and genital bovine herpesviruses.

Oral and genital bovine herpesviruses.


Interactions between Sendai virus and human erythrocytes. Concentrated Sendai virus, when adsorbed to erythrocytes at 4 C, caused invaginations in the plasma membrane. Following elevation of the temperature to 37 C, the plasma membrane became fused with the viral envelope before dissolution of the virions and rupture of the cells. Cell lysis was accompanied by rapid and total loss of hemoglobin to the extracellular space. Following aqueous pyridine extraction, the hemoglobin-free ghosts remaining were found to be devoid of N-acetylneuraminic acid and to have solubility properties different from those of normal erythrocyte ghosts. By the action of viral neuraminidase, bound N-acetylneuraminic acid was also liberated from purified virus receptor substance whose electrophoretic mobility was thereby substantially reduced. Cu(++) selectively inhibited hemolysis and neuraminidase without interfering with hemagglutination and attachment. Neuraminidase appeared to be essential for Sendai virus hemolysis; viral particle size may also be a critical factor in this process.


Abbott's "AA"; admission by appointment.


Changes in daily urinary excretion of follicle-stimulating and luteinizing hormones caused by ethinyl-estradiol and conjugated equine estrogens (Premarin) in low doses.


Malignant melanoma: treatment by endolymphatic radio-isotope infusion.


Nature of anaemia in rheumatoid arthritis. XI. Changes in iron metabolism induced by the administration of corticotrophin.


Effects of somatosensory stimulation on dream content.


Growth of paramyxoviruses in anucleate cells.


Clostridium perfringens meningitis. Report of a case with neuropathological observations.


Reconstitution of respiratory control of succinate oxidation in submitochondrial particles.


Adenyl cyclase activity in human platelets.


Significance of the so-called J-shaped sella in the diagnosis of intracranial aneurysm.


Thermolability of growth promoting substances in the culture medium of Entamoeba histolytica.


Metanephrine: cardiovascular responsiveness in normotensive and renal hypertensive dogs.


Effect of vitamin D2 on hormone-stimulated lipolysis in vitro.


An electrophysiological and psychophysical study of two forms of congenital night blindness.


Recurrence of herpes simplex virus in rabbit eyes: results of a three-year study.


Pulmonary function tests in a Sheffield steel works.


Comparison of reactions of antibodies to rat collagen and to rat kidney in the basement membranes of rat renal glomeruli. By in vivo and in vitro methods of immunofluorescence, antibody to rat collagen and to rat kidney show the same regular, linear fluorescence following the outlines of the renal glomerular capillaries. Absorption of each antiserum with its homologous antigen completely removed the antibody for immunofluorescence, while absorption with the heterologous antigen had no effect. The nephrotoxicity persisted in the anti-kidney serum absorbed with collagen. By pretreatment of frozen normal rat kidney sections with various enzymes followed by immunofluorescence, it was shown that trypsin and hyaluronidase had no effect on the subsequent fluorescence of either antibody; papain reduced the fluorescence; and pepsin and Pronase acted on both antigens so that no fluorescence was present. One preparation of neuraminidase, derived from V. cholerae, reduced fluorescence of both antibodies in some preparations, but the same enzyme derived from influenza virus or C. perfringens had no effect on either. Collagenase completely prevented fluorescence of the antibody to collagen and had no effect on that to rat kidney. The findings in this study show that the antibody to collagen is directed to collagen in rat renal glomerular basement membranes and that the antibody to rat kidney reacts with some antigen other than collagen in these membranes.


Data on the distribution of Clostridium perfringens in the environment of man. Communication 1.


Variability of variola and alastrim virus.


Studies of PPLO infection. V. Inhibition of lymphocyte mitosis and antibody formation by mycoplasmal extracts. Extracts of five arginine-utilizing mycoplasmas inhibit PHA-induced lymphocyte mitosis, while extracts of five glucose-utilizing mycoplasmas do not. Evidence is presented supporting the view that the inhibitory factor is the enzyme arginine deiminase. This enzyme inhibits the reactions of human lymphocytes to antigens as well as PHA, and the secondary production of antibody by rabbit lymph node fragments in vitro. Addition of enzyme to the cells several days after the initial mitotic or antigenic stimulus reduces, but does not abolish, further cellular activity. The production of serum proteins by hepatoma cells is totally unaffected by the mycoplasmal extract. It is concluded that arginine is an essential amino acid for the small lymphocyte, but not for the transformed cell nor for a number of other cell types. Suggestive evidence has been obtained that other enzymes similarly affect lymphocyte reactions.


The action of phenylmethylsulfonyl fluoride on human acetylcholinesterase, chymotyrpsin and trypsin.


Amino acid analysis after degradation of angiotensinII by rat kidney lysosomes.


The possible role of glycoproteins in neural function.


An identification of the radical giving rise to the light-induced electron spin resonance signal in photosynthetic bacteria.


ESR studies of chlorophyll one-electron photochemistry in solution: kinetics of reversible quinone reduction and general redox mechanisms.


Results of treatment of myocardial infarction accompanied by shock in relation to drugs applied.


Experimental studies of the neomycin-induced malabsorption syndrome.


Alcohol dehydrogenase: in vitro studies of its relationship to hepatic lipid synthesis in the rat.


A new influenza A virus infection in turkeys. IV. Experimental susceptibility of domestic birds to virus strain turkey-Ontario 7732-1966.


A new influenza A virus infection in turkeys. V. Pathology of the experimental disease by strain turkey-Ontario 7732-66.


Virus growth inhibition by ethidium chloride and other dyes.


Properties of the CV1 strain of vaccinia virus. I. Studies in cell culture.


The epizootiological importance of foot-and-mouth disease carriers. II. The carrier status of cattle exposed to foot-and-mouth disease following vaccination with an oil adjuvant inactivated virus vaccine.


Virulence, adsorbability, and antigenicity of foot-and-mouth disease virus selected by adsorption with homogenized mouse kidney.


Viral susceptibility of some in vitro cultured tissues from baboons (Papio sp.).


Cytological studies on cytomegalovirus infected mouse cells.


Isolation of adenoviruses from baboons (Papio sp.) with respiratory and enteric infections.


The development of an attenuated foot-and-mouth disease virus vaccine by modification and cloning in tissue cultures of BHK21 cells.


Transformation of hamster embryo fibroblasts by SV40-adeno 7 hybrid virus and the properties of the transformed cells.


Physicochemical studies of equine infectious anemia virus. I. Buoyant density of the virus.


Physicochemical studies of equine infectious anemia virus. II. Sensitivity of the virus to trypsin.


Influence of 6-azauridine on the multiplication of the parainfluenza 3 virus.


Studies on L-ascorbic acid derivatives. II. L-Ascorbic acid 3-phosphate and 3-pyrophosphate.


Data transmission between neurons.

Data transmission between neurons.


Central and reflexogenic cardiovascular actions of prostaglandin E1.


Viremia and immunological responses in horses infected with equine infectious anemia virus.


Semiquantitative estimation of urinary pregnanetriol, pregnanetriolone and tetrahydro S in the investigation of adrenocortical function.


Effects of experimental renal hypertension on aldosterone biosynthesis by rat adrenal tissue.


A radio-bioassay for corticotrophin.

A radio-bioassay for corticotrophin.


Clostridium perfringens food poisoning.

Clostridium perfringens food poisoning.


Effect of veratrine and tetraethylammonium on hippocampal homosynaptic and heterosynaptic post-tetanic potentiation.


Neuroglia and the myelin-bearing cell: a symposium. Immunological factors in experimental allergic encephalomyelitis and multiple sclerosis as revealed by cultures of mammalian nerve tissue.


Relationship between uridine kinase activity and rate of incorporation of uridine into acid-soluble pool and into RNA during growth cycle of rat hepatoma cells.


Studies on morphological precursors of the mouse hepatoma induced with o-aminoaxotoluene. I. Approach to the methodology.


Multiple aneurysms in a case of acromegaly.


Physiological aspects of the motor system.


Peripheral neuropathies following the administration of heterologous immune sera. A critical evaluation.


The suppression of catechol oxidase activity during the enzymic hydroxylation of p-coumaric acid by spinach leaf phenolase.


Hydrolase activities in differentiating plant tissues.


The periodate oxidation of bovine bone sialoprotein, and some observations on its structure. 1. Bovine bone sialoprotein (mol.wt. 23000) contains N-acetylneuraminic acid and N-glycollylneuraminic acid, fucose, galactose, mannose, N-acetylgalactosamine and N-acetylglucosamine residues in the form of a very small number, perhaps one, of highly branched oligosaccharide structures linked covalently to peptide. 2. Periodate oxidation of the sialoprotein results in quantitative destruction only of the sialic acid and fucose residue consistent with the earlier findings of their positions as terminal groups. 3. Terminal sialic acid residues are attached to galactopyranose residues by 2,3-linkages, and to some N-acetylgalactosamine residues (at C-6). 4. Sequential Smith degradation indicates that N-acetylgalactosamine residues may be present as points of branching (linked in C-1, C-3 and C-6) and N-acetylglucosamine residues are located in the inner part of the structure, adjacent to the carbohydrate-peptide bond(s). 5. Mannose residues appear to be linked in the 1,3-positions.


Complex-formation between triphosphoinositide and experimental allergic encephalitogenic protein.


The isolation and properties of experimental allergic encephalitogenic protein. 1. Experimental allergic encephalitogenic (EAE) protein was isolated from ox spinal cord by a modification of the method of Martenson & LeBaron (1966). 2. The protein was examined by acrylamide-gel electrophoresis and its amino acid composition determined. 3. Sedimentation-velocity runs in the ultracentrifuge indicate a molecular weight of about 15100 at pH7.8, and calculation suggests that approx. 137 amino acid residues are present per molecule. 4. Gel-filtration and diffusion studies suggest that the protein is non-globular. 5. Optical-rotatory-dispersion measurements show the protein to have no helical secondary structure even at pH values near to the isoelectric point. In the presence of triphosphoinositide, changes in the optical rotatory dispersion of the protein could be interpreted to mean that it develops a small degree of secondary structure. 6. On treatment with cyanogen bromide the experimental allergic encephalitogenic protein is split into at least two fragments, the larger of which is only about 12% smaller than the parent protein and is antigenically active, and the smaller of which is devoid of antigenic activity.


Effect of iron deficiency on certain iron-dependent enzymes in haemopoietic tissue in bone marrow.


Complex-formation between triphosphoinositide and experimental allergic encephalitogenic protein. 1. Reactions between triphosphoinositide and the basic experimental allergic encephalitogenic (EAE) protein were examined in aqueous solution and in a biphasic solvent system (chloroform-methanol-water, 8:4:3, by vol.). 2. In the absence of salt an insoluble complex (I) is formed containing triphosphoinositide and EAE protein in proportions that represent complete neutralization of lipid and protein at the pH concerned. 3. In the presence of a low concentration (0.05m) of sodium chloride an insoluble positively charged complex (II) forms. It contains triphosphoinositide and EAE protein in a lower concentration ratio than complex I. This complex, which has a constant composition between pH7.5 and pH10, can take up additional micellar triphosphoinositide producing complex I, which can then be solubilized by excess of triphosphoinositide. 4. The complexes are dissociated by more concentrated sodium chloride solutions and low concentrations of calcium chloride, suggesting that they are largely stabilized by electrostatic bonds. The protein recovered after dissociation is immunologically active and has the same electrophoretic mobility as the original. 5. Water-insoluble ternary complexes containing triphosphoinositide, EAE protein and large amounts of phosphatidylcholine can be prepared. From these, chloroform-methanol (2:1, v/v) extracts only phosphatidylcholine. 6. An insoluble ternary complex of Ca(2+) ion, EAE protein and triphosphoinositide can be prepared by adding calcium chloride to a complex I preparation solubilized by excess of triphosphoinositide. 7. EAE protein will also form complexes with other acidic phospholipids, e.g. phosphatidic acid, phosphatidylserine and phosphatidylinositol, but not with phosphatidylcholine or phosphatidylethanolamine. The phosphatidylinositol and phosphatidylserine complexes are chloroform soluble, i.e. proteolipids. 8. The possibility that complexes between EAE protein and acidic phospholipids occur in vivo is discussed. Triphosphoinositide and EAE protein occur in ox brain myelin in approximately the same concentration ratios as they do in complex II, formed at physiological salt concentration and pH.


Experimental and theoretical approaches to neural processing in the central auditory pathway.


Mechanism of induction of cytochrome oxidase in yeast. I. Kinetics of induction and evidence for accumulation of cytoplasmic and mitochondrial precursors.


Effect of ACTH on the metabolism of pregnenolone-7 alpha-3H and cortisol-4-14C in young and elderly men.


Studies on the efficiency of adrenocortical 11 beta-hydroxylation in the human subject.


2,4-diaminopyrimidines. The cyclization of 6-phenacylthio and related derivatives to thieno[2,3-d]pyrimidines and thiazolo[3,2-c]pyrimidines.


Pituitary-adrenal function in arrested hydrocephalus.


Metabolic effects of feeding aspirin to rats at simulated altitude.


Human growth hormone release during sleep: electroencephalographic correlation.


The effect of hyperbaric oxygen on mitochondrial enzymes, lipid peroxides and adenosine triphosphate-induced contraction of mitochondria in organ cultures and cell fractions of rat liver.


Embryonal carcinoma of the pineal region.


Effects of conditioning and some drugs on cold adaptation in rats.


Effect of cold exposure and ACTH on RNA, DNA and protein content in adrenal and liver of rats and ground squirrels.


Kidney function in the high-altitude native.


Active acromegaly with anterior pituitary failure.


EMPIRICISM AND MAGIC IN Aztec pharmacology.


Further experiments with alcohol-treated hepatitogenic serum.


Chronic herpes simplex infection of the ocular adnexa. Persistence of virus in the rabbit after enucleation.


Herpes simplex panuveitis. A clinical report.


Prophylaxis of herpetic keratoconjunctivitis with interferon inducers. Preliminary observations.


Aldolase. II. Spectrophotometric determination using an ultraviolet procedure.


Enzyme content of casts in chronic renal disease.


Alteration by oral contraceptives of normal menstrual changes in plasma renin activity, concentration and substrate.


Pathological lesions, including tumors, in rats after 4,4'-diaminodiphenylmethane and gamma-butyrolactone.


A comparison of the effects of lipolytic and antilipolytic agents on adenosine 3',5'-monophosphate levels in adipose cells as determined by prior labeling with adenine-8-14C. A method for assaying, directly, cyclic 3',5'-adenosine (AMP) in isolated adipose cells is described which consists of measuring formation and accumulation of cyclic AMP-8 which is radiolabeled by 14C.  This assay helped confirm known effects of cyclic AMP and adipose tissue.  It showed that 1) cyclic AMP formation, stimulated by catecholamines, is evident before lipolysis if theophylline is also present; 2) without theophylline, catecholamine-induced lipolysis occurred without elevating cyclic AMP levels; 3) in the absence of norepinephrine, theophylline also induced lipolysis without elevating cyclic AMP levels; and 4) the synergistic effect of theophylline and norepinephrine on lipolysis induction was less marked than the corresponding synergism of theophylline and norepinephrine on elevating cyclic AMP levels.  The assay was also used to confirm the decrease in cyclic AMP levels in adipocytes induced by insulin and prostaglandin E1.  The data confirmed an hypothesis that cyclic AMP exists in different intracellular compartments, only 1 of which, comprising a small fraction of total cellular cyclic AMP, is involved in activation of the hormone-sensitive lipase.  Also found was that polyene antibiotics lowered cellular cyclic AMP levels and markedly increased the levels observed in the medium.


Flavin and pyridine nucleotide oxidation-reduction changes in perfused rat liver. I. Anoxia and subcellular localization of fluorescent flavoproteins.


Structures of the D-galactose oligosaccharides from earthworm cuticle collagen.


Isolation and enzymatic properties of the mitochondrial reduced diphosphopyridine nucleotide dehydrogenase.


Inhibitors and activators of the mitochondrial reduced diphosphopyridine nucleotide dehydrogenase.


Studies on the role of the thymus in viral tumorigenesis. II. Effect of thymectomy on induction of hyperplastic alveolar nodules in BALB-c mice infected with mammary tumor virus at various ages.


Enzymes of nucleic acid metabolism from wheat seedlings. I. Purification and general properties of associated deoxyribonuclease, ribonuclease, and 3'-nucleotidase activities.


Infection with Entameba histolytica and the clinical syndrome of amebiasis. Incidence in a general hospital in Israel.


Properties of pure acyl phosphatase from bovine brain.


Studies on the role of the thymus in viral tumorigenesis. I. Effect of thymectomy on induction of hyperplastic alveolar nodules and mammary tumors in BALB-cfC3H mice.


Interaction of a spin-labeled analogue of nicotinamide adenine dinucleotide with alcohol dehydrogenase. 3. Thermodynamic, kinetic, and structural properties of ternary complexes as determined by nuclear magnetic resonance.


Structure and function of transfer ribonucleic acid. 3. Some properties of a complex between valyl transfer ribonucleic acid synthetase and transfer ribonucleic acid specific for valine.


Association-dissociation reactions of mitochondrial isocitric dehydrogenase induced by protons and various ligands.


Purine N-oxides. 28. The reduction of purine N-oxides by xanthine oxidase.


Findings of antibodies to animal influenza viruses in human sera and their significance for the study of interviral antigenic relationship.


On the production and release of chemical transmitters and related topics in cellular control.


Viral enteric infections among diarrhoeal and non-diarrhoeal infants in Tehran, Iran.


Inhibition of the growth of cultured cells by arginase and soluble proteins from mouse skin.


Complementation and transformation by temperature-sensitive mutants of polyoma virus.


Temperature-sensitive mutants of polyoma virus.


The N-terminal amino acid of an avian leukosis group-specific antigen from avian myeloblastosis virus.


Properties of clonal lines of murine sarcoma virus transformed Balb-3T3 cells.


Concerning the egress of herpes simplex virus from infected cells: electron and light microscope observations.


The influence of pituitary hormones and norepinephrine on the size of adipose cells in organ culture.


A histochemical study on the distribution and localization of beta-glucuronidase activity in cerebral tumors.


Spontaneous activity of cat hippocampal neurons in sleep and wakefulness.


E.S.R. studies of nitroxide probes in lecithin-water systems.


Correlated firing of hippocampal neuron pairs in sleep and wakefulness.


Susceptibility to polymyxin B in penicillin G-induced Proteus mirabilis L forms and spheroplasts. A polymyxin B-resistant strain of Proteus mirabilis was converted into L forms and spheroplasts in the presence of penicillin G. This treatment caused a 400-fold increase in polymyxin B susceptibility. The acquired susceptibility was in the range of the natural susceptibility reported for susceptible gram-negative bacteria ( approximately 1 mug/ml). The high susceptibility to polymyxin B was lost as soon as the spheroplasts and L forms were allowed to reconvert into the bacillary form in penicillin-free media. This behavior is strong evidence that the natural resistance of Proteus strains to polymyxins is due to the impermeability of the outer cell wall structures to these antibiotic substances.


Collagenolytic activity of bacteria.

Collagenolytic activity of bacteria. Actively growing aerobic and anaerobic bacteria were screened by a plate assay, with reconstituted guinea pig collagen as a substrate, for their ability to produce a collagenolytic factor. Collagenolytic activity was not demonstrated among the aerobic organisms tested, with the exception of one strain of Staphylococcus aureus (only when grown under anaerobic conditions). Collagenolytic activity, however, was detected in cultures of Clostridium tetani and Bacteroides species other than B. melaninogenicus. Collagenolytic activity of these organisms could be confirmed by measuring the amount of hydroxyproline liberated from the collagen gel during growth. Although collagenase production by Pseudomonas aeruginosa has been suggested in previous reports, our results were negative. An extracellular fraction of P. aeruginosa was able to hydrolyze a synthetic hexapeptide Cbz-glycyl-l-prolyl-glycyl-glycyl-l-prolyl-l- alanine, but was without detectable effect on reconstituted collagen.


Sensitivity of Pseudomonas aeruginosa to normal serum and to polymyxin. Strains of Pseudomonas aeruginosa isolated from clinical material were very variable in their sensitivity to the bactericidal action of normal serum mediated by the complement system. Fifty per cent killing end points ranged from 0.015 ml to greater than 0.4 ml. Most of the strains with relatively greater sensitivity to serum were isolated from patients with cystic fibrosis. Immunization of rabbits resulted in antisera with enhanced levels of bactericidal antibody, except with one strain which was resistant to the bactericidal action of normal serum and antiserum. When P. aeruginosa was cultivated at 41 C instead of at 37 C, it was significantly more sensitive to serum and to several antibiotics, thereby implicating fever as a host defense mechanism in Pseudomonas infections. In contrast to their heterogeneity to serum bactericidal activity, the strains were relatively homogeneous in their sensitivity to polymyxin, with no apparent association between their sensitivity to the two antimicrobial agents.


Malate utilization by a group D Streptococcus: physiological properties and purification of an inducible malic enzyme. Growth of Streptococcus faecalis in the presence of l-malate resulted in the induction of a "malic enzyme" [l-malate:nicotinamide adenine dinucleotide (NAD) oxidoreductase (decarboxylating), E.C. 1.1.1.39]. Synthesis of the malic enzyme did not appear to be subject to catabolite repression by intermediate products of glucose or fructose dissimilation. However, malate utilization was inhibited during growth in the presence of glucose or fructose. The purified enzyme was specific for malate as substrate and NAD as cofactor. Mn(+2) or Mg(+2) was required for optimal activity and NH(4)Cl stimulated the reaction rate. Several lines of indirect evidence suggested that the streptococcal malic enzyme was involved primarily with energy production and not biosynthesis.


Effect of copper deficiency on chick bone collagen and selected bone enzymes.


Potassium activated phosphatase from human red blood cells. The mechanism of potassium activation. 1. The kinetic behaviour of the K-activated phosphatase in human red blood cell membranes has been investigated. The concentration of Mg required to give optimal activation is independent of substrate and K concentration, suggesting that Mg combines with the enzyme at a site that is independent of and non-interacting with the substrate and K sites.2. The effects of K are competitively antagonized by Na. Ouabain in suitable concentrations selectively abolishes the activating effect of K.3. Comparison between the hydrolysis of acetylphosphate by intact red cells and by fragmented ghosts suggests that the active site for the substrate is only accessible at the internal surface of the cell membrane.4. The plot of the total rate of p-nitrophenylphosphate hydrolysis versus substrate concentration can be fitted at any K concentration by a rectangular hyperbola. The effect on the total rate of increasing K concentration is exerted mainly on the apparent affinity for the substrate, which increases about 5 times as K goes from 0 to 55 mM. There is also a much smaller increase in the maximum velocity (about 1.3 times) for the same range of K concentrations.5. If the difference between the activity in the absence and in the presence of K is plotted as a function of substrate concentration, the curves obtained are no longer hyperbolic but pass through a maximum and then tend to a lower value.6. This kinetic behaviour can be explained much more easily by assuming that a single enzyme is responsible for the hydrolysis of the substrate in the presence and in the absence of K. A simple kinetic model based on this assumption was developed and when experimentally determined constants were fitted into the equations that predict its behaviour a reasonably good agreement between theory and experiment was obtained.7. In the ;single enzyme' model inhibitors that selectively abolish the K-dependent activity would act by blocking the combination of the enzyme with K. A simple treatment based on this idea was developed for the case of Na and its predictions were fulfilled by the experimental results.8. In the ;single enzyme' model the K-coupled hydrolysis is always larger than the difference between the rates in the presence and in the absence of K, and when K concentration is non-limiting the K-coupled rate is equal to the total rate.


Correlation between transmission and structure in avian ciliary ganglion synapses. 1. Extracellular responses from post-ganglionic axons of pigeon and chick isolated ciliary ganglia were elicited by stimulation of the presynaptic nerve. Intracellular recordings were also obtained from newly hatched pigeon and chick ganglion cells. The fine structure of ganglia from pigeons of various ages was examined with the electron microscope.2. In ganglia from chick embryos and pigeons up to 10 days old, the extracellular response was unimodal with a long latency and could be blocked by the addition of D-tubocurarine (D-TC) or hexamethonium to the bathing solution. A bimodal extracellular response appeared in pigeons about 10 days after hatching. Only the second peak of the response could be blocked by D-TC or hexamethonium. The response recorded from 22 to 26-day-old pigeons was similar to that seen in the adult.3. The intracellular recordings from ganglion cells of 2-week-old pigeons exhibit two post-synaptic potentials elicited by presynaptic stimulation. The first post-synaptic potential appears to be due to current flow through the ganglion cell during the presynaptic action potential. The second is chemically mediated. In pigeons from 1 to 6 days old, only the second post-synaptic potential is observed.4. The presynaptic terminals in the 4-day-old birds were in the form of calyces. In pigeons 7 days old or older, boutons appeared. The boutons were presumably formed as a result of cleavage of calyciform nerve terminals. Myelin was seen first in the 7-day-old pigeon, was well developed in the 16-day-old bird, and persisted in the adults.5. In adult ganglia, the first component of the extracellular response decreased and was finally abolished after 10-12 hr of superfusion with Tyrode solution. The second component of the response increased concomitantly. The only anatomical change noted in the ganglia after soaking was the disruption and separation of the myelin lamellae from each other and from around the ganglion and presynaptic terminals.6. It is concluded that the myelin is necessary for electrical transmission in the pigeon ciliary ganglion.


Changes in carotid sinus baroreceptor activity induced by angiotensin.


Effects of some cancer-chemotherapeutic agents, especially cyclophosphamide, on an SV40 sarcoma in rat.


Comparative effects of tumor extracts on lymphocyte transformation in peripheral blood cultures of healthy persons and patients with breast cancer.


Calcifying epithelial odontogenic tumour.


Corticotrophin and its synthetic analogues.


The two kinds of doctor.

The two kinds of doctor.


Localization of the hypotenisve effect of 2-(2-6-dichlorophenylamino)-2-imidazoline hydrochloride (St 155, Catapresan).


An attempt to type some of the untyped enteroviruses with antisera prepared against two of these untyped viruses.


Neonatal smiling and rapid eye movement states.


Acute hepatitis due to Herpes simplex virus in an adult. A case is described of herpetic hepatitis in a pregnant woman with primary herpetic stomatitis. Intranuclear inclusion bodies and virus particles were found in hepatocytes, and herpes virus was isolated from a liver biopsy and from oral swabs but not from blood. From rising titres of neutralizing and complement-fixing antibody it is concluded that the oral infection was a primary one. Factors predisposing to the hepatitis are discussed.


Electron microscopic observations on the virus particles in Herpes simplex encephalitis. Cerebral cortex from eight fatal cases of Herpes simplex encephalitis has been studied by electron microscopy. The diagnosis had been based on clinical findings, necropsy evidence of bitemporal necrosis, and histological confirmation of intranuclear inclusions with a lymphocytic inflammatory response, even though virus cultures were unsuccessful. Virus particles were seen predominantly in nuclei in six of these cases, utilizing tissue which had been preserved in formalin for up to four years or had been embedded in paraffin. Although demonstration of these particles is only indicative of the herpes group and not specifically of Herpes simplex, electron microscopy of either biopsy or postmortem material may be a useful adjunct in making the diagnosis and can be accomplished before virological studies have been completed.


Labeled dopamine concentration in human adrenal medulla and in neuroblastoma.


Sodium metabolism.

Sodium metabolism.


Acute peripheral neuropathy.

Acute peripheral neuropathy.


Purification and characterization of bluetongue virus.


Morphology of bluetongue virus.

Morphology of bluetongue virus.


Multiple antigenic components of the group-specific antigen of the avian leukosis-sarcoma viruses.


The formation of variants with a reversion of properties of transformed cells. II. In vitro formation of variants from polyoma-transformed cells.