Altered specificity of transfer-ribonucleic acid nucleotidyltransferase in the presence of manganese. 1. s-RNA nucleotidyltransferase incorporated CMP into phosphodiesterase-treated s-RNA more rapidly in the presence of Mg(2+) (10mm) than in the presence of Mn(2+) (2mm). UMP was incorporated more rapidly in the presence of Mn(2+), and at high ionic strength the incorporation of CMP was also more rapid in the presence of Mn(2+). 2. The capacity of phosphodiesterase-treated s-RNA for CMP, UMP and AMP was increased in the presence of Mn(2+). Terminal sequences of more than one UMP or AMP residue were synthesized, but these atypical reactions were inhibited when CTP was added. CMP was incorporated rapidly to form -pCpC terminal sequences and then more slowly as longer chains were formed, but very little CMP was incorporated into s-RNA-pCpCpA. 3. CMP was incorporated into phosphodiesterase-treated 5s RNA and ribosomal RNA to form short chains of polyC attached to the primer RNA. This reaction was inhibited by the presence of s-RNA. 4. A small Mn(2+)-dependent incorporation of CMP was also primed by poly(A).(U) and poly(C).(I).


Polyribosomes in normal Krebs 2 ascites tumor cells and in cells infected with encephalomyocarditis virus.


Fractionation of nuclear ribonucleic acids from tumor and homologous tissue on a methylated albumin column by the use of salt and temperature gradients.


Polymerization of the adenosine 5'-diphosphate ribose moiety of NAD by rat liver nuclear enzyme.


Action of cyclic phosphodiesterase from Escherichia coli on tobacco mosaic virus ribonucleic acid.


The action of snake venom phosphodiesterase on liver ribosomal ribonucleic acids.


An extension of the naphthalene disulphonate method for mammalian nucleic acids.


Relationship between photosynthetic and oxidative phosphorylations in chromatophores from light-grown cells of Rhodospirillum rubrum.


Phosphate metabolism in the electric organ.


Inhibitors of NADH-ubiquinone reductase from mitochondria.


Cytochrome oxidase and its derivatives. VII. Further observations on the compound formed by the reaction of ferrous cytochrome c oxidase with oxygen and hydrogen peroxide and on its reactions.


The low-temperature spectral properties of mammalian cytochrome oxidase. I. The enzyme in intact rat-liver mitochondria.


The occurrence of cytochromes in nuclei isolated from rat thymus.


Comparative phosphorescence quenching of DNA's of different composition. Small amounts of paramagnetic cations quench the phosphorescence of DNA. Although the emission intensity is monotonic with increasing thymine content, the quenching efficiency is not. The cations quench from phosphate sites.


Neuronal spike trains and stochastic point processes. I. The single spike train. In a growing class of neurophysiological experiments, the train of impulses ("spikes") produced by a nerve cell is subjected to statistical treatment involving the time intervals between spikes. The statistical techniques available for the analysis of single spike trains are described and related to the underlying mathematical theory, that of stochastic point processes, i.e., of stochastic processes whose realizations may be described as series of point events occurring in time, separated by random intervals. For single stationary spike trains, several orders of complexity of statistical treatment are described; the major distinction is that between statistical measures that depend in an essential way on the serial order of interspike intervals and those that are order-independent. The interrelations among the several types of calculations are shown, and an attempt is made to ameliorate the current nomenclatural confusion in this field. Applications, interpretations, and potential difficulties of the statistical techniques are discussed, with special reference to types of spike trains encountered experimentally. Next, the related types of analysis are described for experiments which involve repeated presentations of a brief, isolated stimulus. Finally, the effects of nonstationarity, e.g. long-term changes in firing rate, on the various statistical measures are discussed. Several commonly observed patterns of spike activity are shown to be differentially sensitive to such changes. A companion paper covers the analysis of simultaneously observed spike trains.


Neuronal spike trains and stochastic point processes. II. Simultaneous spike trains. The statistical analysis of two simultaneously observed trains of neuronal spikes is described, using as a conceptual framework the theory of stochastic point processes.The first statistical question that arises is whether the observed trains are independent; statistical techniques for testing independence are developed around the notion that, under the null hypothesis, the times of spike occurrence in one train represent random instants in time with respect to the other. If the null hypothesis is rejected-if dependence is attributed to the trains-the problem then becomes that of characterizing the nature and source of the observed dependencies. Statistical signs of various classes of dependencies, including direct interaction and shared input, are discussed and illustrated through computer simulations of interacting neurons. The effects of nonstationarities on the statistical measures for simultaneous spike trains are also discussed. For two-train comparisons of irregularly discharging nerve cells, moderate nonstationarities are shown to have little effect on the detection of interactions.Combining repetitive stimulation and simultaneous recording of spike trains from two (or more) neurons yields additional clues as to possible modes of interaction among the monitored neurons; the theory presented is illustrated by an application to experimentally obtained data from auditory neurons.A companion paper covers the analysis of single spike trains.


Abortion in cattle caused by experimental infection with infectious bovine rhinotracheitis virus.


Ribonuclease-cyclic phosphodiesterase system in Escherichia coli.


Effects of bretylium, guanethidine and bethanidine on the cat's pad sweat glands.


The effects of angiotensin on the concentration of plasma free fatty acids in man.


Some vascular effects of angiotensin in the rat.


Chemical fractionation of antiviral plants.


Association of glycine with spinal interneurones.


Inhibition of motoneurones by iontophoresis of glycine.


Buoyant density of herpes simplex virus in solutions of caesium chloride.


Polycation effect on virulence of nonpathogenic r+ variant of the encephalomyocarditis virus.


Hypophosphatasia in an adult. A case report.


Reciprocal interference between mouse mammary tumour virus and leukaemia virus.


Disappearance of angiotensin from the circulation of the dog.


Effect of rotenone on the alcohol dehydrogenase of yeast mitochondria.


Electron microscopy of monkey kidney cells infected with both SV40 and measles virus.


Caesium chloride in the preparation of membrane fractions from human cerebral tissue.


Inhibition of fatal anaphylaxis in mice inoculated with a virus-induced leukaemic tumour extract.


Effect of saxitoxin on the end plate of frog muscle.


Nicotinamide adenine dinucleotide and the metabolism of ethanol and acetaldehyde.


Inhibition of Rous sarcoma virus and chicken leukosis viruses by 1-adamantanamine hydrochloride.


Parainfluenza 1 (Sendai) antibodies and mumps vaccination.


Semiautomated determination of fluoride in urine.


Distribution of neuraminidase among food-poisoning strains of Clostridium perfringens. A survey was made to determine the distribution of the enzyme neuraminidase among 76 strains of Clostridium perfringens. Representative strains from each toxigenic type (A to F) and atypical C. perfringens type A food-poisoning strains of both American and English (Hobbs types) origin were tested. Both the American food-poisoning and nonfood-poisoning associated cultures consisted of both neuraminidase-positive and -negative strains. Furthermore, American strains which could not be differentiated from the original Hobbs cultures consisted of both neuraminidase-positive and -negative representatives. In contrast, the English (Hobbs) strains uniformly failed to produce an active intracellular or extracellular neuraminidase. No enzyme activity was detected in these strains when cultures were grown in different growth media, when grown in the presence of substrate (neuraminlactose), or upon extended incubation of enzyme preparations with substrate. With the exception of a type F strain, representative strains of the other toxigenic types (A to F) produced neuraminidase; 85% of the typical type A strains contained the enzyme.


The steroid shield in ophthalmology.

The steroid shield in ophthalmology.


Sporulation of Clostridium perfringens in a modified medium and selected foods. A modified sporulation medium for Clostridium perfringens was formulated in which a larger number of spores were produced than in SEC broth and in which spores of greater heat resistance were produced than in Ellner's medium when it was also used as the suspending medium. This modified medium consisted of 1.5% peptone; 3.0% Trypticase; 0.4% starch; 0.5% NaCl; and 0.02% MgSO(4). The addition of 0.1% sodium thioglycolate and 0.0001% thiamine hydrochloride was optional. The optimal temperature for sporulation of five strains was 37 C in comparison with 5, 22, and 46 C. Sporulation had occurred by 6 hr and was essentially complete after 20 hr at 37 C. Noyes veal broth without glucose also supported the formation of heat-resistant spores but in smaller numbers than did the modified medium. Very low numbers of spores, or none, were produced under the same conditions in pea or tuna slurries.


The use of silica gel in source testing.


Quantitative studies on fabrics as disseminators of viruses. 3. Persistence of vaccinia virus on fabrics impregnated with a virucidal agent. Eight compounds were tested in vitro for virucidal and antiviral activity against poliovirus and vaccinia virus. These compounds included five quaternary ammonium salts, two bromosalicylanilides, and neomycin sulfate, an antibiotic. None of the compounds was active against poliovirus, but virucidal activity was demonstrated against vaccinia virus with three of the quarternary ammonium compounds: n-alkyl (C14, C12, C16) dimethyl benzyl ammonium chloride, di-isobutyl cresoxy ethoxy ethyl dimethyl benzyl ammonium chloride monohydrate, and n-alkyl (60% C14, 30% C16, 5% C12, 5% C18) dimethyl benzyl ammonium chlorides plus n-alkyl (50% C12, 30% C14, 17% C16, 3% C18) dimethyl ethylbenzyl ammonium chlorides. Wool blanketing, wool gabardine, and cotton sheeting materials were impregnated with the first of the above virucidal compounds, and the persistence of vaccinia virus on these fabrics was compared with the persistence of the agent on nonimpregnated fabrics of the same type held at 25 C in 35 and 78% relative humidity. No virus could be recovered from the chemically treated fabrics at any time after virus exposure, whereas the virus persisted as long as 4 weeks on nonimpregnated materials. Viable vaccinia virus was also found to persist less than 1 day on a cotton fabric finished with a wash-and-wear modified triazone resin. Poliovirus persisted less than 5 days on this wash-and-wear fabric.


Incorporation of liquid hydrocarbons into agar media.


Some observations on the hazards of corticosteroid therapy in patients with inflammatory bowel disease.


Effects of calcium-deficient perfusion on isolated mesenteric arteries.


Transmission of sensory information through the lemniscal pathway during sleep.


Presynaptic and postsynaptic inhibition of transmission of somatic afferent volleys through the cuneate nucleus during sleep.


Vestibular influences during sleep. V. Vestibular control on somatic afferent transmission in the cuneate nucleus during desynchronized sleep.


Identification of sulfur as component of the EPR signal at g equals 1.94 by isotopic substitution.


Metabolite repression of fructose 1,6-diphosphatase in yeast.


Polynucleotide phosphorylase: structure and mechanism of action.


Comparative studies on the activity of carboxylases and other enzymes in relation to the new pathway of photosynthetic carbon dioxide fixation in tropical grasses. 1. The activity per unit of chlorophyll of certain carboxylases, and of other enzymes involved in photosynthesis, was determined in leaf extracts of the tropical grasses, sugar-cane, maize and sorghum, and compared with the activities for wheat, oat and silver-beet. Maximum rates of photosynthetic carbon dioxide uptake were also measured for comparison with enzyme activities. 2. Phosphopyruvate carboxylase activity was about 60 times greater in the tropical grasses than in wheat, oat and silver-beet and was severalfold higher than the rates of photosynthetic carbon dioxide uptake. Most of the enzyme was located in the chloroplast fraction of cell extracts. 3. Phosphopyruvate carboxylase was apparently the major photosynthetic carbon dioxide-fixing enzyme in the tropical grasses, although malic enzyme may contribute to a lesser extent. 4. Tropical grasses contained less than one-tenth of the ribulose diphosphate carboxylase activity present in wheat, oat and silver-beet. For the tropical grasses this activity, determined with a saturating concentration of bicarbonate, was approx. 10% of the rate of photosynthesis. 5. The fraction-1 protein content of leaf extracts paralleled the ribulose diphosphate carboxylase activity. 6. In contrast, the activity of several other enzymes of the Calvin cycle was similar in the different species examined.


The role of aminoacetone in L-threonine metabolism by Bacillus subtilis.


A continuous recording technique for the measurement of carbon dioxide, and its application to mitochondrial oxidation and decarboxylation reactions. 1. A technique is described for continuously recording the concentration of carbon dioxide in mitochondrial suspensions. 2. The oxidation of palmitoylcarnitine by rat-liver mitochondria inhibits the oxidation of pyruvate and isocitrate, and stimulates the carboxylation of pyruvate. 3. These effects of palmitoylcarnitine oxidation are reversed by the uncoupling agent pentachlorophenol. 4. The effects of palmitoylcarnitine oxidation and pyruvate oxidation on the acylation of mitochondrial coenzyme A and reduction of nicotinamide nucleotides were measured. 5. Control mechanisms are discussed for the interactions between palmitoylcarnitine oxidation and the tricarboxylic acid cycle in rat-liver mitochondria.


Ubiquinone deficiency in an auxotroph of Escherichia coli requiring 4-hydroxybenzoic acid. 1. Escherichia coli 156:53D2 synthesized ubiquinone only when the growth medium was supplemented with 4-hydroxybenzoate acid. 2. Little or no vitamin K(2) was formed by the mutant under the growth conditions employed, in contrast with wild-type strains. 3. In the mutant ubiquinone deficiency was correlated with low respiration and with low particulate NADH-oxidase and NADH-cytochrome b(1)-reductase activity. 4. Preincubation of ubiquinone-deficient particles with ubiquinone-30 largely restored the NADH-oxidase and NADH-cytochrome b(1)-reductase activities. 5. Various NADH-dye-linked reductases which may be associated with NADH dehydrogenase were not affected by the absence of ubiquinone. 6. The succinate-oxidase complex was less affected than the particulate NADH oxidase by ubiquinone deficiency. 7. A pathway for electrons in the NADH-oxidase complex of the auxotroph of E. coli is proposed and its relationship to the pathway in the wild-type strain is discussed.


A nuclear magnetic resonance study of substrate binding by alcohol dehydrogenases.


Liver enzyme changes in the developing rat.


The effect of iodination upon the catalytic and regulatory activities of fructose 1,6-diphosphatase.


Rat liver D-beta-hydroxybutyrate dehydrogenase. I. Partial purification and general properties.


Rat liver D-beta-hydroxybutyrate dehydrogenase. II. Lipid requirement.


Mitochondrial respiration under conditions of varying osmolarity.


Utilization of respiratory substrates in calf-retina mitochondria.


Oscillations of nucleotides and glycolytic intermediates in aerobic suspensions of Ehrlich ascites tumor cells.


Dietary response of various key enzymes related to glucose metabolism in normal and diabetic rat liver.


Phosphates and phosphatases in preosseous cartilage.


The composition of bovine cartilage and bone.


Acid resistance and infectivity of food-poisoning Clostridium perfringens.


Sensitivity to, and production of interferon by three variants of Mengo virus.


Certain physical and chemical properties of two murine leukemia viruses.


Studies on the toxicity of polymyxin B to blue-green algae.


On the contractile response of the isolated rat uterus to prostaglandin E1.


Tetraethylammonium-induced contractions of frog's skeletal muscle. I. General considerations.


Tetraethylammonium-induced contractions of frog's skeletal muscle. 3. Mechanism of action by calcium release.


A micromethod for measurement of renin in the plasma and kidney of rats.


Note on the preparation of renin substrate.


A simplified preparation of phosphatidyl inositol. A method is described for the rapid isolation of phosphatidyl inositol from soybean phosphatides (Asolectin). The product is obtained pure as the crystalline sodium salt.


Phosphatidyl glycerophosphate phosphatase. An enzyme (phosphatidyl glycerophosphate phosphatase) that catalyzes the formation of phosphatidyl glycerol from phosphatidyl glycerophosphate has been rendered soluble by treatment of the particulate fraction of E. coli with Triton X-100 in the presence of EDTA, and has been partially purified. The enzyme is specific for phosphatidyl glycerophosphate and does not catalyze the hydrolysis of other simple phosphomonoesters. It requires Mg(++) for activity and is inhibited by sulfhydryl agents. Some other properties of the enzyme are also described.


Internal standards in the estimation of acetyl-CoA in liver extracts. The necessity for adding an internal standard to liver extracts during fluorimetric estimation of acetyl-CoA by the malic dehydrogenase-citrate synthase reaction is demonstrated. Addition of acetyl-CoA completely compensates for the inhibitory action of some tissue components. Values for hepatic acetyl-CoA in fed and fasted rats are given.


Concurrent measurement of renin and angiotensin in the circulation of the dog.


Enzyme mechanism of aminoacetone metabolism by micro-organisms.


Diurnal variation of adrenocortical function in hypophysectomized rats with multiple heterotopic pituitary homografts.


PAS-positive erythroblasts in kidney diseases.


Hereditary methemoglobinemia due to DPNH-methemoglobin reductase deficiency: report of a family.


Hematological and serological behaviour of chicks inoculated with myeloblastosis virus.


Cytological diagnosis of generalized cytomegalic inclusion disease.


Lipolytic action of 3,3'5-triiodo-L-thyronine, a cyclic AMP phosphodiesterase inhibitor.


Functional activity of DNA and DNA polymerase during thymine starvation of Escherichia coli 15T.


Studies on alkaline phosphatase. Inhibition by phosphate derivatives and the substrate specificity. 1. The kinetics of inhibition of calf-intestinal alkaline phosphatase by inorganic phosphate, fluorophosphate, inorganic pyrophosphate, beta-glycerophosphate and adenosine 5'-triphosphate in the range pH8-10 were investigated. The reference substrate was 4-methylumbelliferyl phosphate. 2. The inhibitions were ;mixed' in that both K(m) and V were affected, but the competitive element was by far the stronger. 3. In each case the pH profile for the competitive K(i) was similar to the pH profile for K(m). Since the K(m) and K(i) values both change 100-fold over the pH range 8-10, it is concluded that the inhibitors compete with the substrate for the same active site. 4. It was also found that the enzyme preparation hydrolysed fluorophosphate, pyrophosphate and adenosine 5'-triphosphate as readily as it hydrolysed 4-methylumbelliferyl phosphate and beta-glycerophosphate. Each pH-activity curve, however, had a different shape, but with the exception of pyrophosphate the activity approached the same maximum value at high pH. 5. Attempts to separate the phosphomonoesterase and pyrophosphatase activities by column chromatography were not successful, and the results of other experiments listed suggest that the two activities are a property of the same enzyme. 6. The effect of Mg(2+) ions is briefly mentioned: the phosphomonoesterase activity is enhanced whereas the pyrophosphatase and adenosine triphosphatase activities are strongly inhibited in the presence of excess of Mg(2+) ions.


Inhibition of glycolytic enzymes of normal and neoplastic liver by free fatty acids.


Inhibition of liver microsomal inorganic pyrophosphate-glucose phosphotransferase and glucose 6-phosphate phosphohydrolase activities by metal-binding agents.


Control of citrate formation in rat liver by the nicotinamide-adenine dinucleotide redox state.


Nicotinamide-adenine dinucleotide-specific isocitrate dehydrogenase from pea mitochondria.


Characteristics of the binding of rotenone in the respiratory chain and the inhibition sites of amytal and piericidin A.


The phospholipid composition of embryonic chick liver microsomes. 1. The phospholipid composition of hepatic microsomal fractions from different developmental stages of embryonic chick was established. The major components were phosphatidylcholine (approx. 66%), phosphatidylethanolamine plus phosphatidylserine (approx. 21%) and sphingomyelin (approx. 9%). 2. There were no significant changes in the phospholipid composition during embryonic development from 9 to 20 days. 3. When microsomal subfractions were prepared it was found that the smooth-microsomal fractions (Ia and Ib) had a significantly greater sphingomyelin content than the rough-microsomal fraction (II). This was compensated by a lower phosphatidylcholine content in fractions Ia and Ib and an increase of phosphatidylcholine in fraction II. 4. The significance of the differences in the phospholipid composition of smooth and rough microsomes is discussed with particular reference to the origin and interrelation of smooth and rough endoplasmic reticulum.


Inhibition of alcohol dehydrogenase from yeast by pyridine. 1. Inhibition by pyridine of reduction of NAD by ethanol in the presence of yeast alcohol dehydrogenase was studied at 25 degrees in 60mm-glycine buffer (K(+), pH9.3). 2. The apparent Michaelis constant for ethanol increases linearly and that for NAD increases non-linearly with pyridine concentration. 3. Rates, v, observed in the presence of pyridine are lower than the values calculated from the effect of pyridine on the two apparent Michaelis constants and are described by the expression V/v={1+5.8[pyridine]}x{1+0.016(1+124 [pyridine)]/[EtOH]}x{1+0.00019(1+3.3[pyridine]+110 [pyridine](2))/[NAD]}. 4. Mixed inhibitor studies with pyridine and N(1)-methylnicotinamide chloride in 40mm-pyrophosphate buffer (Na(+), pH8.2) indicated little interaction of pyridine with the ;pyridinium site' of the dehydrogenase (interaction constant, alpha, 2.1). 5. The possible competition of ethanol and pyridine for a zinc atom in the active centre of yeast alcohol dehydrogenase is discussed.


Isolation and amino acid composition of human angiotensin I. 1. Angiotensin, the most powerful pressor substance known, suspected to be a causal substance in renal hypertension and previously isolated from animal sources, has now been isolated from human sources and the amino acid composition was analysed. 2. The procedures followed in the successful isolation of human angiotensin include: (a) preparation of stable materials to obtain maximum formation of human angiotensin; (b) a relatively selective adsorption of the formed angiotensin on Dowex 50W (X2); (c) gel filtration through Sephadex G-25; (d) cation-exchange chromatography on CM-cellulose; (e) anion-exchange gel filtration on DEAE-Sephadex A-25; (f) molecular-sieve chromatography through Bio-Gel P-2. 3. The homogeneity of the human angiotensin isolated was confirmed by paper and thin-layer chromatography and paper electrophoresis. 4. The biological activity observed indicates the substance isolated to be human angiotensin I. 5. The amino acid analysis suggested the following proportional composition: Asp, 1; Pro, 1; Val, 1; Ile, 1; Leu, 1; Tyr, 1; Phe, 1; His, 2; Arg, 1. This composition is similar to that of horse angiotensin I, i.e. isoleucine(5)-angiotensin I.


The contaminating effects of cephalins in estimating non-esterified fatty acids.


Effects of insulin and phospholipase A on glucose transport across the plasma membrane of free adipose cells.


Enzyme specificity in the stimulation of glucose transport in free adipose cells by phospholipases.


On the nature of endogenous substrate in rat-liver mitochondria.


Inhibition by formaldehyde of energy transfer and related processes in rat-liver mitochondria.


Nitrite metabolism by skeletal muscle mitochondria in relation to haem pigments.


The cytochrome system of Azotobacter vinelandii.


Highly purified cytochromes C derived from the diatom, Navicula pelliculosa.


Purification and some properties of cytochrome c dervied from the marine worm, Dendrostomum zostericolum.


NADH oxidation by hypoxanthine dehydrogenase of avian kidney.


Cholinergic inhibition in the cerebral cortex.


Clostridium perfringens as a pathogen of cattle: a literature review. Reports of Cl. perfringens as a pathogen for cattle are reviewed and discussed.


Effect of incubation temperature and serum content in agar overlay on plaque production by foot-and-mouth disease virus. The numbers of plaques produced by foot-and-mouth disease virus in primary cultures of calf kidney cells conditioned to 24, 30, and 37 C were essentially the same if virus was absorbed at 37 C. Adsorption was as effective at 30 as at 37 C in cultures conditioned to the respective temperature, but 24 C was less effective under this condition. However, when the adsorption temperature for cultures conditioned to 37 C was decreased to 30 or 24 C, fewer plaques were produced than in cultures conditioned to and maintained at the lower temperatures during absorption. Comparison of the number of plaques produced in cultures overlaid with nutrient agar containing from 0.5 to 10% bovine serum revealed no difference in relation to serum concentration. However, plaque size was related directly to the concentration of serum in the overlay.


A survey of the alimentary tract of cattle for Clostridium perfringens. Contents of the rumen, abomasum, ileum, and colon of 100 fattened cattle were examined for the presence of Cl. Perfringens. Liquid medium iniculated with each sample of gut content was tested for the presence of toxins of Cl. Perfringens. Identification of Cl. Perfringens was based on atmospheric requirements for growth, colonial morphology, and stormy fermentation in litmus milk. Identification of toxins was based on neutralization tests in guinea pigs and mice.Cl. Perfringens was isolated from 202 of 399 samples. In 105 additional cultures, colonies characteristic of Cl. Perfringens were present but could not be isolated in pure culture.Cl. Perfringens type D toxin was identified in only one culture, which was inoculated with ileum contents. Type A toxin was identified in eight of the 24 samples from the one lot of samples in which no type A antitoxin was used. There were no identifications of toxigenic types B, C, or E. The results indicate that an isolation from necropsy specimens of untyped Cl. Perfringens or type A Cl. Perfringens is in itself of little significance. The infrequency of occurrence of the other toxigenic types in this survey of healthy cattle indicates that recovery of these types from necropsy specimens may be of more significance in determining the cause of death.


Glyconeogenesis, fructose-1,6-diphosphatase and phosphoenolpyruvate carboxykinase activities of Ascaris lumbricoides adult muscle and larvae.


Enzymatic softening of connective tissue sheaths to aid microelectrode penetration.


Studies on the antiviral activity of formycin.


Discharge patterns of neurones supplying tonic abdominal flexor muscles in the crayfish.


Histochemical patterns in the proventriculus of the developing chick.


Nature of thalamo-cortical relations during spontaneous barbiturate spindle activity. 1. The relation between thalamic and cortical spontaneous spindles was investigated in cats anaesthetized with barbiturates.2. Simultaneous recordings with multiple electrodes in the thalamus and cortex revealed a high correlation between thalamic and cortical spindle activity, both with regard to the occurrence of the spindles as well as to their individual waves, provided a critical location of the electrodes was secured. The results indicate a point-to-point relation between a group of thalamic cells and a small cortical area to which these cells project. In the spontaneous rhythm, the cortical columns are probably individually controlled by a thalamic rhythmic entity. This point-to-point relation was found in all the major sensory projection systems and in one thalamic ;association' nucleus and its corresponding ;association' cortex.3. Cortical barbiturate spindles appeared either as local spindles in a restricted cortical area or as compound spindles in several areas. Spindles recorded from electrodes separated by 2 mm or more were clearly different with regard to intraspindle wave frequency, duration, and the time of start and stop of the spindle. These differences increased with increasing distance between the electrodes, and were most pronounced when the corticograms of the two hemispheres were compared.4. Spontaneous spindle activity interfered with orthodromic transmission through n. ventralis posterolateralis (VPL) and medial geniculate nucleus (MG), judged by depression of the thalamic and cortical responses to peripheral nerve volleys or clicks. Such inhibition required the afferent volley to be delivered at a particular time of the spontaneous oscillations. Further, orthodromic volleys reset the rhythmic spindle waves in the appropriate thalamic and cortical areas.5. These findings lead to a new concept of the thalamic pace-maker function. During barbiturate anaesthesia, small assemblages of thalamic neurones seem to have the ability to generate independent rhythmic discharges, and thereby control the rhythm of the particular cortical column to which this thalamic group projects.6. During barbiturate anaesthesia, many facultative pace-makers seem to be present in the thalamus. The total number may be as large as 25,000-35,000. Usually, many of these rhythmic thalamic units beat in synchrony or near synchrony.


Aldosterone and angiotensin action on water absorption in the rat jejunum.


Post-tetanic increase in frequency of miniature end-plate potentials in calcium-free solutions.


Ionic requirements of synaptic transmitter release.


Micro-iontophoretic studies on neurones in the cuneate nucleus. 1. Cuneate cells in anaesthetized cats were strongly excited by L-glutamate, and somewhat less by D-glutamate; cells which receive afferents from hair receptors were particularly sensitive.2. Glutamate could be used to demonstrate post-synaptic inhibitory inputs from the dorsal column, the medial lemniscus and the frontal cortex.3. Many cuneate cells were also strongly excited by adenosinetriphosphate (ATP); this was probably due to the chelating action of ATP, as citric acid was also quite effective.4. gamma-Aminobutyric acid (GABA) readily blocked all forms of spontaneous and evoked activity, except antidromic invasion of cuneothalamic neurones; cells which receive proprioceptive afferents were particularly sensitive to GABA. Glycine had a comparable effect.5. Acetylcholine (ACh), catecholamines, histamine, 5-hydroxytryptamine (5-HT) and an extract containing substance P mostly had only weak depressant actions. Cholinergic and mono-aminergic mechanisms are probably not very significant in the cuneate.6. These results are consistent with the possibility that glutamate and GABA (or glycine), or some closely related compounds, are the main excitatory and inhibitory transmitters in the cuneate nucleus.7. If ATP is released from afferent nerve endings, it could also play a significant role in excitation.


Crystalline vitamin D3 for the prevention of milk fever in cattle.


The safety of massive doses of vitamin D3 in the prevention of milk fever.


Cytochemical and autoradiographic investigations on normal and myelomatous plasma cells.


The significance of mutual adhesiveness of cells in the histological pattern of cancer tissues.


The function of the adrenal cortex in mice with spontaneous adrenocortical lipid depletion.


A modification of the biologic test for tuberculosis with silica-treated guinea pigs.


A tissue-specific shift in lactate dehydrogenase sub-band patterns of the house mouse.


New surgical approach to nasopharyngeal angiofibromas.


The subcellular localization of factor VII (proconvertin) in rat liver.


The release of catecholamines from the adrenal medulla by peptides.


[Enzymatic activities in the small intestine of the lamb. (Acid and alkaline phosphatases, glucose-6-phosphatase, alpha-D-methylglucosidase, invertase and lactase)].


The incidence and significance of bovine herpesvirus (infectious bovine rhinotracheitis) antibodies in the sera of aborting cattle. The results of bovine herpesvirus (IBR-IPV) neutralization tests conducted on the sera of 463 animals with a prior history of undiagnosed abortion and 331 control animals with no history of abortion are reported. One hundred and thirty-one (28.3 per cent) of the aborting and 105 (31.7 per cent) of the control animals, were found to harbour antibody to this virus. No significant seasonal or gestational incidence could be determined but some variation in the annual incidence was apparent. On the basis of these results it appears unlikely that this virus is responsible for a significant proportion of the undiagnosed cases of bovine abortion in Quebec and Ontario, where the animals tested were located. The problems involved in substantiating a diagnosis of abortion due to bovine herpesvirus are briefly discussed.


Histological observations on pituitary ACTH-cells, adrenal cortex, and the corpuscles of stannius of the european eel (Anguilla anguilla L.).


Nephroblastoma. A clinical study of 26 cases.


The new method of suturing traumatic peripheral nerve paralysis.


Isolation of a collagenolytic enzyme from Mycobacterium tuberculosis.


Lack of cytochrome oxidase in yeast cells deficient in pantothenate.


Tissue interactions in the development of ptilopody and brachydactyly in the chick embryo.


A comparative study on avian adrenocortical response to exogenous and endogenous corticotropin.


The margin of safety of neuromuscular transmission. 1. The margin of safety for neuromuscular transmission in the tibialis and sartorius muscles of the cat has been determined by measuring the ratio by which end-plate depolarization produced by succinylcholine, decamethonium, octamethonium or iodocholine is antagonized, in the presence of neuromuscular block produced by tubocurarine, gallamine or DF-596. The estimate of the margin of safety was independent of the particular drugs chosen for the measurement.2. To produce threshold block to indirect stimulation once every 10 sec, a fractional occupancy by the antagonist of 0.76 +/- 0.05 (S.D.) was required; for nearly complete block, an occupancy of 0.917 +/- 0.16 (S.D.) was required. These figures correspond to factors of safety of 4.1 and 12 for the most sensitive and the most resistant groups of fibres respectively.3. The interaction between the agonists and the antagonists, when tested over a wide range of dosage, did not conform with the conditions of full competitive equilibrium. It was concluded that this arose, not because of some interfering non-competitive process, but because, during the relatively brief exposure to agonist, the equilibrium between the antagonist and the receptors is not significantly disturbed. An analysis of this condition of quasi-equilibrium is given. A correction downwards of the direct estimates of the margin of safety is required, but this proves to be small, about 8%, and may not be significant.4. The safety factor diminished when the motor nerve had been cut more than 5 hr; it is suggested that this represents an early sign of nerve degeneration.5. With dog sartorius muscle, results similar to those in the cat were obtained. But for deep block in the rabbit, the safety factor was only about 4.6. The existence of a substantial margin of safety influences considerably the interpretation of the time course of action of blocking drugs, and of comparisons between responses to nervous excitation and drug injection.


Transformation of polyploid hamster cells by polyoma virus.


Growth of cell-strains and primary cells on micro-carriers in homogeneous culture.


Silica in the wall of Pediastrum.

Silica in the wall of Pediastrum.


Amnesia induced by scopolamine and its temporal variations.


On the nature of free radicals induced by X-rays in puric bases.


Sensitized photodimerization of thymine in DNA.


The histocompatability-2 locus and susceptibility to bittner virus borne by red blood cells in mice.


Complement-fixing antibodies in sera of human and nonhuman primates to viral antigens derived from Burkitt's lymphoma cells.


The genetic heterogeneity of simian virus 40.


The significance of abnormal bacterial proliferation in the gastrointestinal tract after gastric surgery.


Intraspinal tumours in children.

Intraspinal tumours in children.


Adrenergic transmission of the carotid chemoreceptor impulses in the toad.


Occasional appearance of diphosphopyridine nucleotide in urine of patients with progressive muscular dystrophy.


Bovine papular stomatitis, pseudocowpox and milker's nodules.


Management of benign trophoblastic tumors.


Achromatic regions of tetrazolium stained starch gels: inherited electrophoretic variation.


Accidental Q-switched laser lesion of human macula.


Newer compounds in therapy of herpes simplex keratitis.


Experimental stromal herpes simplex keratitis in rabbits.


Herpes genitalis; clinical and virological studies.


Ultrathin frozen sections. II. Demonstration of enzymic activity. Endogenous enzyme activity can be readily and routinely demonstrated in ultrathin, frozen sections for electron microscopy. The procedure employed to obtain the best structural preservation as well as enzyme activity in thin sections involved fixation in glutaraldehyde, embedding in thiolated gelatin or pure gelatin, partial dehydration in glycerol, and sectioning in a cryostat at -35 degrees C with a slightly modified Porter-Blum microtome on which the tissue is maintained at -70 degrees C and the knife at -23 degrees C. Kidney cortex was used as test tissue, but a few other organs were occasionally used. Thin sections were floated on the surface of several incubation media routinely employed for enzyme cytochemistry. Positive, specific reactions were obtained for alkaline phosphatase in kidney brush border, for adenosine triphosphatase in brush border and in basal membranes of distal tubules, for acid phosphatase and esterase in lysosomes, and for NADH diaphorase in mitochondria. Mitochondrial ATPase was sporadically evident only in the distal tubule of the kidney. Localizations of enzyme activity reported by other technical approaches were confirmed and in some cases somewhat improved.


Ultrastructural demonstration of cytochrome oxidase activity by the Nadi reaction with osmiophilic reagents. A new method for the subcellular and cytochemical demonstration of cytochrome oxidase has been developed with the introduction of N-benzyl-p-phenylenediamine (BPDA) and the discovery that indoanilines are osmiophilic. These indoanilines produced upon oxidation of BPDA in the presence of naphthols are highly colored compounds that yield electron-opaque coordination polymers of osmium (osmium black) that are amorphous, insoluble in water, and in organic solvents. The best methods for preparing rat tissue were in decreasing order: fixation in formaldehyde solution, fresh tissue slices, and frozen sections of fresh or fixed tissue. Ultrathin sections were counterstained by bridging with the thiocarbohydrazide-osmium tetroxide (T-O) procedure for enhancing underlying membranous structures. Cytochrome oxidase activity was noted primarily in mitochondria and occasionally in sarcotubules of heart, in mitochondria and occasionally in infoldings of the plasma membrane of renal tubular cells, and in mitochondria and, to a great extent, in endoplasmic reticulum of hepatic cells. Cytochrome oxidase activity produced deposits in droplet form, whereas dehydrogenase activity resulted in uniform staining of mitochondrial cristae, as recently demonstrated with an osmiophilic tetrazolium salt. Even more recently we have succeeded in demonstrating cytochrome oxidase activity in nondroplet staining on mitochondrial cristae with an osmiophilic benzidine-type reagent that apparently polymerizes upon oxidation (to be published later).


Lack of glutethimide effect of adrenal.

Lack of glutethimide effect of adrenal.


Estrogen metabolism in the human. 3. A comparison between females, studied during the first and second halves of the menstrual cycle, and males.


On the localization of some phosphatases in three different segments of the proximal tubules in the rat kidney.


Fatal Mycoplasma pneumoniae infection with isolation of organisms from lung.


Pertussis.

Pertussis.


Group A streptococci, mycoplasmas, and viruses associated with acute pharyngitis.


Rhinovirus infections in an industrial population. II. Characteristics of illness and antibody response.


Endoplasmic reticulum and the origin of microbodies in fetal mouse liver.


Correlation of ultrastructure and function following hypothermic preservation of canine kidneys.


Studies on aldehyde fixation. Fixation rates and their relation to fine structure and some histochemical reactions in liver.


The renin-angiotensin-aldosterone system in sodium depletion.


Dermal erythropoiesis in neonatal infants. A manifestation of intra-uterine viral disease.


The relationship of menopausal muscular dystrophy to other diseases of muscle. A study of 17 cases.


Studies on characteristics of poliovirus. II. Plaque-forming capacity of attenuated poliovirus strains in human and monkey kidney cell cultures.


Studies on characteristics of poliovirus. 3. Investigation of d, rct, h and n markers of poliovirus isolated after vaccination with the Type 1, Chat strain.


Effect of thymectomy on polyoma tumor induction in CF 1 mice.


Inoculation of a novel type of rhinovirus (H.S.) to human volunteers.


Genetic transfer of guanidine resistance from type 2 to type 1 poliovirus.


The rabiesviruses--a new group?

The rabiesviruses--a new group?


Factors associated with the response of the cell to herpes simplex virus infection.


Antibody response and antigenic difference between high and low oncogenic variants of SE polyoma virus.


Synthesis of uncoated viral RNA following picornavirus infection.


The organismic theory of tumour formation.


The importance of SH-groups for enzymic activity. V. The coenzyme-binding capacity of pig heart lactate dehydrogenase, isozyme I, after inhibition by various maleinimides.


Gamma-butyrolactone and gamma-hydroxybutyric acid. II. The pharmacologically active form.


The histologic identity of adenoma sebaceum and solitary melanocytic angiofibroma.


Studies on lipoamide dehydrogenase of Bakers' yeast. 8. Inhibition of enzyme activity by its immune antiserum.


Facilitation at the frog neuromuscular junction during and after repetitive stimulation.


Ultrastructural localization of some phosphatases in the prothoracic gland of the insect Leucophaea maderae.


Inhibitor o,p'-DDD and vitamin D and dihydrotachysterol function in the chick.


Renal function and the renin-angiotensin system in the isolated perfused kidney.


Clinical features of adenoviral pneumonia in Air Force recruits.


Herpes simplex virus and paralysis of rabbits. Activation of the paralysis by adrenalin.


Pure and mixed hilus-cell tumours of ovary. Arris and Gale Lecture delivered at the Royal College of Surgeons of England on 14th November 1966.


Electron microscopic study of the distribution of the mouse leukemia virus (gross) in genital organs of virus-injected C3Hf mice and of AK mice.


Studies with angiotensin in nephrogenic diabetes insipidus.


Chromosomes of "minimal deviation" hepatomas and some other transplantable rat tumors.


A difference in sites of DNA synthesis in virus-induced (Shope) and in chemically induced epidermal tumors of rabbit skin.


Cellular analysis of liver carcinogenesis: the induction of large hyperplastic nodules in the liver with 2-fluorenylacetamide or ethionine and some aspects of their morphology and glycogen metabolism.


Intracellular distribution of 5'-ribonuclease and 5'-phosphodiesterase in the Novikoff hepatoma ascites cells.


Evidence for the Calvin cycle and hexose monophosphate pathway in Thiobacillus ferrooxidans. The enzymes of the Calvin reductive pentose phosphate cycle and the hexose monophosphate pathway have been demonstrated in cell-free extracts of Thiobacillus ferrooxidans. This, together with analyses of the products of CO(2) fixation in cell-free systems, suggests that these pathways are operative in whole cells of this microorganism. Nevertheless, the amount of CO(2) fixed in these cell-free systems was limited by the type and amount of compound added as substrate. The inability of cell extracts to regenerate pentose phosphates and to perpetuate the cyclic fixation of CO(2) is partially attributable to low activity of triose phosphate dehydrogenase under the experimental conditions found to be optimal for the enzymes involved in the utilization of ribose-5-phosphate or ribulose-1,5-diphosphate as substrate for CO(2) incorporation. With the exception of ribulose-1,5-diphosphate, all substrates required the addition of adenosine triphosphate (ATP) or adenosine diphosphate (ADP) for CO(2) fixation. Under optimal conditions, with ribose-5-phosphate serving as substrate, each micromole of ATP added resulted in the fixation of 1.5 mumoles of CO(2), whereas each micromole of ADP resulted in 0.5 mumole of CO(2) fixed. These values reflect the activity of adenylate kinase in the extract preparations. The K(m) for ATP in the phosphoribulokinase reaction was 0.91 x 10(-3)m. Kinetic studies conducted with carboxydismutase showed K(m) values of 1.15 x 10(-4)m and 5 x 10(-2)m for ribulose-1,5-diphosphate and bicarbonate, respectively.


Distribution of cytochrome c peroxidase activity in wild-type and petite cells of bakers' yeast grown aerobically and anaerobically. Studies of mitochondrial biogenesis in yeast have been hampered by a lack of suitable membrane markers in anaerobically grown cells subsequently grown in air. Cytochrome c peroxidase activity and subcellular location was studied to determine whether it would be a useful marker for an analysis of mitochondrial formation. Cytochemical tests revealed enzyme reaction product on all mitochondrial membranes in aerobically grown wild-type cells. Anaerobically grown wild-type and all petite cultures contained cytochrome c peroxidase cytochemical reaction deposits on abundant cytoplasmic membranes and on the few mitochondrial profiles which also were seen in the electron photomicrographs. Biochemical studies corroborated the cytochemistry because mitochondrial fractions were greatly enriched in cytochrome c peroxidase activity for aerobically grown wild-type cultures, but petite and anaerobically grown wild-type cultures showed higher enzyme activities in supernatant fractions than was present in the corresponding particulate fractions after differential centrifugation. Evidence from low-temperature microspectroscopy, spectrophotometric assays of mitochondrial enzyme activities, and electron microscopy showed mitochondrial formation during the time required for preparation and lysis of spheroplasts from anaerobically grown cultures. The data were interpreted as indicating that cytochrome c peroxidase was an oxygen-inducible enzyme, and that there was a developmental relationship between enzyme-reactive membranes of mitochondria and cytoplasm during the period of respiratory adaptation.


Fine-structure analysis of intercellular and intracellular mitochondrial diversity in Saccharomyces cerevisiae. Crosses were made between haploid wild-type and suppressive petite strains of bakers' yeast to obtain zygotes for analysis of mitochondrial heterogeneity. Wild-type x petite zygotes contained about 40% noncristate mitochondria when immediate mating mixtures were examined. The frequency of defective mitochondria had decreased to an average of 9.2% in 1-week-old zygote isolate cultures, and to 4.4% in slant cultures 1.5 years after initial zygote isolation. The latter value was not significantly different from values obtained with wild x wild zygotes of either age. The noncristate mitochondria were of two types: one lacking inner membrane invaginations or elaborations and the other containing concentrically arranged loops of inner membrane. The significance of these two types of respiration-deficient mitochondria is unknown. The gradual decrease in frequency of noncristate mitochondria, perhaps due to selection pressures in mixed chondriomes, was discussed as a further indication of the semiautonomous nature of the yeast organelle.


Role of S-adenosylmethionine in methionine biosynthesis in yeast. Extracts of Saccharomyces cerevisiae were used to develop a cell-free system capable of converting the beta-carbon of serine into the methyl group of methionine. No requirement for either S-adenosylmethionine or S-adenosylhomocysteine could be demonstrated for net methionine biosynthesis. Growth of the cells in B(12) did not affect the reaction. The mechanism for the methylation of homocysteine in yeast appears to be similar to the non-B(12) system in Escherichia coli.


Transfer of growth inhibition between normal and virus-transformed cells: autoradiographic studies using marked cells.


Interaction between normal and transformed bovine fibroblasts in culture. I. Cells transformed by Rous sarcoma virus.


QUuantitative investigation on the effect of puromycin on the aggregation of trypsin- and versene-dissociated chick fibroblast cells.


The production of 11-deoxycortisol (Reichstein's substance S) in patients with adrenocortical hyperfunction.


Electron-microscopic studies on a double-stranded beaded filament of embryonic collagen.


Corticosteroid secretion in kangaroos (Macropus canguru major and M. (Megaleia) rufus).


Some experiences with chemical inactivants of foot-and-mouth disease virus.


Cumulative changes in the antigenic properties of herpes simplex virus from persistently infected cell cultures.


Immunochemical studies of foot and mouth disease. I. Complement fixation reactions with isolated antigenic components.


The cellular localization of mercury-203 chlormerodrin in astrocytomas.


Kaposi's varicelliform eruption.

Kaposi's varicelliform eruption.


Nephrotoxicity of antibiotics.

Nephrotoxicity of antibiotics.


Studies on silica shock in the rabbit. II. The possibility of release of "contact factor" by colloidal silica, and vasoconstriction of pulmonary embolism as the cause of death.


Cellular age changes in insects.

Cellular age changes in insects.


Effects of phenol solutions on the nerve roots of the cat: an electrophysiological and histological study.


Changes in lipid metabolism with ageing.


The effect of ageing upon collagen metabolism.


Replication of herpes simplex virus in arginine-free media. I. Effect of arginine-deficiency in different tissue culture cells.


Replication of herpes simplex virus in arginine-free media. II. DNA synthesis in infected and non-infected human embryonic fibroblast and HeLa tissue culture cells.


Phosphatidyl serine requirement of (Na+-K+)-activated adenosine triphosphatase from rat kidney and brain.


The synthesis of taurocholic and glycocholic acids by preparations of human liver. II. An analysis of the stimulating effect of the L fraction.


The anomeric configuration of L-rhamnose in uridine 5'-diphosphate rhamnose and thymidine 5'-diphosphate rhamnose.


The synthesis of uridine diphosphate xylose by particulate preparations from mouse mast-cell tumors.


Electron spin resonance studies of the chloranil-cysteine, dichlone-cysteine and dichlone-glutathione reactions.


The mechanism of the phosphoenolpyruvate synthase reaction.


A model for neuron firing with exponential decay of potential resulting in diffusion equations for probability density.


Determination of serum and plasma angiotensinase activity.


Respiratory enzymes in mantle homogenates of Crassostrea virginica and the effects of Minchinia nelsoni (MSX), and Cochlodinium helicoides on activity levels.


Glutamic acid dehydrogenase activity in muscles of dystrophic mice.


Mechanisms of sympathetic regulation of arterial smooth muscle.


Serum desoxy-ribose compounds in experimental myocardial infarction.


Effect of lidoflazine on norepinephrine, angiotensin and serotonin responses in isolated smooth muscle preparations.


Histochemical nature of the motor endplate.


Priming activity for RNA synthesis of chromatin from transplantable hepatomas and livers of tumor-bearing rats.


Skin grafts in Donryu rats with and without acquired resistance to Yoshida sarcoma and ascites hepatomas.


Biochemical changes in tumor cells caused by antitumor agents. 3. Further studies on the effects of antitumor agent on nad'ase and a few lysosomal enzymes of Yoshida sarcoma cells.


Effect of pregnancy on growth of rat ascites tumors.


The pharmacology of haloperidol.

The pharmacology of haloperidol.


Potentiation of secretin stimulation of the pancreas. 1. In fasted anaesthetized cats which were secreting pancreatic juice at a steady slow rate in response to continuous intravenous infusion of secretin, stimulation of the dorsal vagus trunk increased the rate of secretion, the rate of output of amylase and the concentration of bicarbonate in the juice.2. The increase in rate of secretion and in concentration of bicarbonate was reduced but not abolished by atropine. It was unaffected by adrenergic blocking agents, but abolished by hexamethonium.3. No increase in rate of secretion of secretin-stimulated juice was observed on cervical stimulation of vagus nerves in which the efferent fibres had degenerated after supra-nodose section of the nerves. It is concluded that the increase in secretion rate is mediated by ;atropine-resistant' efferent vagal fibres.4. Pancreozymin, antral extracts and histamine increased the rate of secretion of secretin-stimulated juice. Pancreozymin and antral extracts also increased the rate of amylase output. These effects of pancreozymin and antral extracts were not abolished by atropine or hexamethonium.5. As vagal stimulation pancreozymin, antral extracts and histamine have little or no effect on the resting pancreas of the anaesthetized cat, it is concluded that they exert a potentiating effect on secretin. It is suggested that the mechanism of this potentiation is an increase in pancreatic blood flow, which facilitates the supply of secretin to the gland.


Responses of the pyramidal tract to stimulation of the baboon's motor cortex. 1. The arm area of the baboon's precentral motor cortex was stimulated by brief surface-anodal pulses, and the discharge of the corticospinal tract (the ;pyramidal tract waves') was recorded by an electrode resting on the dorsolateral surface of the cervical spinal cord.2. Some properties of the pyramidal tract waves were described, and they were also studied in relation to the firing of single cortico spinal fibres.3. The results led to the conclusion that the later pyramidal tract waves (the ;I waves') were almost exclusively due to a semi-synchronous repetitive discharge of the same fast cortico spinal fibres as those responsible for the initial wave (the ;D wave').4. Some problems concerning the origin and significance of the I waves were discussed.


Change in enzyme pattern after cross-innervation of fast and slow skeletal muscle.


"Pock-less" rabbit pox.

"Pock-less" rabbit pox.


Purification and physico-chemical characterization of Ecbovirus type SA-I.


Dental abrasion and alveolar bone loss in the white rat. IV. The importance of the consistency of the diet and its abrasive components.


The reactivity of the clotting mechanism and fibrinolysis in cerebral thrombotic disease.


Muco-epidermoid carcinoma of the lacrimal gland.


The effect of 4,4'-methylenedianiline on adrenal corticosterone secretion in the rat.


Quantitative analysis of the acquired resistance in tumor-bearing hosts.


Growth acceleration of a mouse cell line after infection with Friend leukemia virus.


Evaluation of Sabin live poliovirus vaccine in Japan. II. Clinical, virologic and immunologic effects of vaccine in children.


Humoral conditioning for necrosis. II. The challengers.


Auditory-evoked responses in cats with tenotomized middle ear muscles during sleep.


A method for assessment of skeletal maturity.


The nutrition and metabolism of mycoplasma: Progress and problems.


Electron spin resonance studies on heart muscle succinic dehydrogenase: substrate analogues.


Biosynthesis of phytoene from isopentenyl and farnesyl pyrophosphates by a partially purified tomato enzyme system.


Isolation and characterization of uridine diphosphate fructose from tubers of jerusalem artichoke (Helianthus tuberosus L.).


Diphosphopyridine nucleotide-linked aldehyde dehydrogenase. 3. Sulfhydryl characteristics of the enzyme.


Acetyl phosphatase in brain microsomes: a partial reaction of Na+ plus K+ ATPase.


ESR and crystallization studies on iron-free xanthine oxidase.


Vitamin D3-induced calcium-binding protein: partial purification, electrophoretic visualization, and tissue distribution.


Gel filtration of nucleic acids on sphere-condensed agarose.


Studies on a 32S component of nuclear RNA.


Coexistence of infectious bronchitis and infectious laryngotracheitis.


Mycoplasma gallisepticum in a commercial laryngotracheitis vaccine.


The influence of co-precipitants on the solvent denaturation of double-stranded RNA.


Interaction of glutamate dehydrogenase with fluorescent dyes.


The binding of NADH by glutamate dehydrogenase as measured by polarization of fluorescence.


An E.S.R. study of free radicals formed by reaction of nucleotides and their constituents with hydrogen atoms.


Low temperature magnetic susceptibility of ceruloplasmin.


Ultracentrifuge and electron microscope studies on the structure of mitochondrial DNA.


Synthesis of low molecular weight ribonucleic acid in tumour cells.


Electron microscope study of the denaturation of human papilloma virus DNA. I. Loss and reversal of supercoiling turns.


Electron microscope study of the denaturation of human papilloma virus DNA. II. The specific location of denatured regions.


An adenovirus isolated from the feces of mice. II. Experimental infection.


Zonal increase in lactic dehydrogenase activity in the senescent human aorta.


Serial passages of equine infectious anemia virus in horse leukocyte culture.


Physicochemical studies on equine infectious anemia virus. Examination of purification methods.


Propagation and titration of equine infectious anemia virus in horse leukocyte culture.


Pathological studies on bone marrow in equine infectious anemia. I. Macroscopical findings on whole longitudinal sections of bone marrow.


Half-lives of peptides and amines in the circulation.


Interferon and the interaction of allogeneic normal and immune lymphocytes with L-cells.


Suppression of viral and chemical carcinogenesis by means of artificial heterogenization.


Localization of lactic dehydrogenase isozymes in lysosomal fraction of the neutrophil of normal human blood.


Chronic hemodialysis in "unselected" patients.


Mechanisms of steatorrhea in the Zollinger-Ellison syndrome.


Adrenocortical responsiveness to ACTH and angiotensin II in hypophysectomized dogs and dogs treated with large doses of glucocorticoids.


An effect of adrenocorticotrophic hormone on adrenal cholesterol accumulation.


Effect of hypothalamic lesions on pituitary-adrenocortical responses to histamine and methopyrapone.


Quantitative studies on the iodinated components secreted by the rat thyroid gland as determined by in situ perfusion.


Hypsarrhythmoid EEG features in infants and young children with subdural hygromas.


Application of the renin-antirenin assay in experimental hypertension: a preliminary report.


Paramagnetic resonance spectrum of irradiated glycylglycine HCl.


Gynandroblastoma of the ovary: review of 8 cases from the ovarian tumor registry.


Changes of intestinal absorptive cells during maturation: an electron microscopic study of prenatal, postnatal, and adult guinea pig ileum.


Gradient centrifugation of viruses in colloidal silica.


Primary linitis plastica of the colon: report of a case and review of the literature.


Studies on the synthesis of herpes simplex virions.


Alkaline disruption of poliovirus: kinetics and purification of RNA-free particles.


Isolation of an avian leukosis virus associated with the Harris strain of Rous sarcoma virus.


Increased susceptibility to foot-and-mouth disease virus of bovine lung cells transformed by polyoma virus.


Water vapor electrolysis.

Water vapor electrolysis.


Photodynamic inactivation of Japanese encephalitis virus.


Oxidative phosphorylation in crithidia fasciculata.


The intracellular distribution of mercury in rat liver after methoxyethylmercury intoxication.


Hepatic metabolism and enzyme activity in acute ethanol administration.


Effects of actinomycin D and mitomycin C on the degradation of deoxyribonucleic acid and polydeoxyribonucleotide by deoxyribonucleases and venom phosphodiesterase.


Equilibrium centrifugation of DNA, RNA, and poliovirus in density gradients of cesium oxalate and cesium acetate.


A contagious pox disease in monkeys.

A contagious pox disease in monkeys.


Studies on cytochrome a. XVI. Significance of the molecular state of cytochrome a for oxidase activity.


The sequence of elution of plant organic acids from silica gel chromatographic columns.


A technique for the removal of the active silica gel layer from Eastman Chromagram sheet and its subsequent replication.


Separation of polymyxin B, D, E and M by thin-layer chromatography.


Studies on Rous sarcoma virus in mice. I. Establishment of an ascites sarcoma induced by the Schmidt-Ruppin strain of Rous sarcoma virus in C3H-He mouse.


Viral ribonucleic acid of the Schmidt-Ruppin strain of Rous sarcoma virus. I. On the virus recovery from the medium of the in vitro cultivation of chicken wing web Rous sarcoma cells and the isolation of viral ribonucleic acid (RNA) from purified viral particles.


Effects of alcohols and acetone on the neuromuscular junction of frog.


New site of action of some narcotics, carbamate and alcohol on the respiratory enzyme system.


Interlocked stresses in cartilage.

Interlocked stresses in cartilage.


Variation in physiological properties of crustacean motor synapses.


Transmission from excitatory nerves to the smooth muscle cells of the rat seminal vesicles.


Isolation of SV40 virus and its effect on renal function in African green monkeys.


Electron paramagnetic resonance of heme at intermediate oxidation states of cytochrome c oxidase.


A virus released by "nonproducing" Rous sarcoma cells.


Interaction among avian tumor viruses giving enhanced infectivity.


Tumor virus RNA's.

Tumor virus RNA's.


Symposium on RNA viruses and neoplasia. Comment.


Follow-up study on corticotrophin treatment of multiple sclerosis.


Infection of muscle cultures from various species with oncogenic DNA viruses (SV40 and polyoma).


An investigation of carriers of Entamoeba histolytica.


Comparison of selected characteristics of four strains of infectious bovine rhinotracheitis virus.


Infectious bovine rhinotracheitis-infectious pustular vulvovaginitis virus infection in pregnant cows.


Antigenic analysis of bovine enteroviruses through studies of the kinetics of neutralization.


Cellular changes in the adrenal medulla of the calf following injection of adrenocorticotropic hormone and sodium depletion.


Secretory rates of aldosterone in sodium-depleted calves.


Serologic study of reovirus infection in dogs.


Development of an antigen in visceral lymphomatosis and splenic lymphoid foci.


Histopathologic and hematologic changes in moribund stages of chicks infected with T-virus.


Cytologic studies in polyoma virus-infected mouse embryo cells.


Comparison of a bovine enterovirus and foot-and-mouth disease virus.


Regrowth of primary cell cultures after infection with foot-and-mouth disease virus.


Lyophilizing foot-and-mouth disease virus at low drying temperature.


Cowpox and paravaccinia.

Cowpox and paravaccinia.


Deaths associated with respiratory tract infection in childhood.


Problem patients with vulvar malignancy.


Hypokalaemic alkalosis and hyperplasia of the juxtaglomerular apparatus without hypertension or oedema.


Gluconeogenesis during hibernation and arousal from hibernation.


Heart excitatory and inhibitory substances in molluscan ganglia.


Lipofibroma of the median nerve in the palm.


Macrodactyly.

Macrodactyly.


Comparative study of histochemical patterns in non-neoplastic and neoplastic gastric epithelium: a study of Japanese in Hawaii.


Triphosphoinositide phosphomonoesterase activity in nerve cellbodies, neuroglia and subcellular fractions from whole rat brain.


Electron spin resonance of two glycine peptides irradiated at 77 degrees K.


Free radicals and their interactions in solid molecular mixtures.


Hepatomas.

Hepatomas.


Getting on their nerves.

Getting on their nerves.


Development of tachyphylaxis to aspartyl and not asparginyl angiotensin II in the rat.


Six cases of acrylamide poisoning.

Six cases of acrylamide poisoning.


Muco-epidermoid metaplasia and argentaffin cells in nephroblastoma.


Studies on the mechanism of hormonal induction of the melanoblast-melanocyte transformation in organ culture.


Histamine-sensitising factor of Bordetella pertussis differentiated from immunogens by neutralisation and passive protection tests.


Ultrastructural observations on the Harvey mouse leukaemia-sarcoma virus.


Histo-enzymatic studies of experimental bacterial endocarditis.


Phosphatase reaction of coagulase-negative staphylococci and micrococci.


Enzyme studies of the periosteum in experimental lathyrism.


Evaluation of peripheral and central components of action of angiotensin on the sympathetic nervous system.


Effects of an adrenal 11-beta, 17-alpha hydroxylase inhibitor in juvenile-onset diabetes mellitus.


On the mechanism of ganglionic blockade by methacholine.


Muscarinic transmission of preganglionic impulses to the adrenal medulla of the cat.


On the mechanism of neuromuscular blocking action of bretylium and guanethidine.


Rehabilitation of the stroke patient.

Rehabilitation of the stroke patient.


Psychopharmacologic drugs.

Psychopharmacologic drugs.


Diagnosis of abdominal masses.

Diagnosis of abdominal masses.


Eruptive hidradenoma and syringoma. Histological, histochemical, and electron microscopic studies.


Methotrexate. Effects on the enzymes of carbohydrate metabolism in psoriasis.


Methylation of DNA in vitro: enzymic activity from different bacterial strains on DNA from various sources.


Hepatocellular carcinoma in infancy and childhood in Ibadan, Western Nigeria.


Incidence of lung tumours in LX mice exposed to (1) free radicals; (2) SO2.


Metabolic degradation of 3,4-benzopyrene in the cultures of normal and neoplastic fibroblasts.


Electron spin resonance of irradiated DNA.


Separation of chitin oligosaccharides by thin-layer chromatography.


The effect of metyrapone on aldosterone secretion in man.


Hormonal studies of a benign interstitial cell tumour of the testis producing androstenedione and testosterone.


Chemical and electron microscopic studies of the black pigment of the human lung.


Carcinoid syndrome associated with pancreatic tumors.


Hyponatremia in acute polyneuropathy. Four cases with the syndrome of inappropriate secretion of antidiuretic hormone.


Charcot-Marie-Tooth disease.

Charcot-Marie-Tooth disease.


Benign mucous gland adenoma of the bronchus.


Inhalation therapy and postural drainage for the treatment of cystic fibrosis.


Renal neoplasm in the newborn infant.

Renal neoplasm in the newborn infant.


Physiology of selenite reduction by enterococci. II. Characterization of selenite inhibition and its reversal by ascorbate.


Further studies on the kinetic serum-neutralization (McBride) test for polioviruses.


Cancer of the male breast: forty cases.

Cancer of the male breast: forty cases.


Factors affecting the production of foot-and-mouth disease virus in deep suspension cultures of BHK21 clone 13 cells.


Serological studies on infections by respiratory viruses of the inhabitants of Tristan da Cunha.


Neurosurgical experiences with herpes simplex encephalitis.


The incidence of Herpesvirus hominis antibody in the population.


Acute respiratory disease and meningococcal infection in army recruits.


Vincristine sulfate in management of Wilms' tumor. Replacement of preoperative irradiation by chemotherapy.


Testicular gas gangrene after hernia repair with cord division.


Capillary-tube scanner for mechanized microbiology. A photoelectric scanner measures growth in agar-filled capillaries and gives a new approach to microbiology. Growing microcolonies of bacteria can be detected by their light-scattering property. We have described a system in which growing bacteria in glass capillaries filled with nutrient agar are counted as the capillaries move through a narrow beam of light. Increased counts after incubation indicate the presence of viable organisms in the original sample; the equipment can detect colonies when they grow to a diameter of 8 microm. Aerobic, anaerobic, and facultative organisms can grow in the capillary environment. The sensitivities of several organisms to antibiotics were determined by adding antibiotics to the agar before the capillaries were filled.


The ultrastructure of human mammary fibroadenoma.


Copper distribution in the neonatal rat.


Acidemia and catecholamine output of the isolated canine adrenal gland.


Effects of angiotensin infusion on renal function in the unanesthetized rat.


Iodized salt in the prevention of endemic goiter: a world-wide survey of present programs.


Minimal infective dose of attenuated poliovirus for man.


Inositide metabolism in rabbit hereditary ataxia.


Intraspinal tumors of infancy and childhood: a review of 19 surgically verified cases.


Adrenocorticotrophic action of long-acting tetracosactrin compared with corticotrophin-gel.


Morphological transformation of mouse embryo cells in vitro by murine sarcoma virus (Harvey).


Labelled fluoroborate in the stomach of rats.


Drugs and the Coombs antiglobulin test.

Drugs and the Coombs antiglobulin test.


Clinical useful antimicrobial agents. General considerations concerning chloramphenicol, erythromycin and allied drugs, the polymyxins, bacitracin, vancomycin, ristocetin, cycloserine, the cephalosporins, the chemotherapeutic drugs and the antimycotic drugs.


Clinically useful antimicrobial agents. Dosage and mode of administration.


Clinically useful antimicrobial agents. Untoward reactions.


Effect of ACTH on the metabolism of radioactive C-21 steroids by quartered rat adrenals.


Neuronal localization of dopamine and 5-hydroxytryptamine in some mollusca.


Histochemistry of the periodontal ligament. II. The phosphatases.


Experimental production of diarrhea and its prevention by Malethamer in monkeys.


Inactivation of infectious bovine rhinotracheitis virus by calcium alginate wool swabs.


A sensitive assay for egg-white and bacteriophage-induced lysozyme.


Acceleration of cytochrome oxidase synthesis specific to zygotes from crosses between complementary respiratory deficient mutants of S. cerevisiae.


The conversion of deoxyuridine 5'-monophosphate to deoxyuridine 5'-triphosphate in normal and regenerating rat liver.


ACTH stimulation of adenyl cyclase in adrenal homogenates.


Ferredoxin-activated fructose diphosphatase in isolated chloroplasts.


Solubilization of enzyme forming ADPR polymer from NAD.


Metabolism of lysine in rat liver.

Metabolism of lysine in rat liver.


The structure of the serotype 2 capsular polysaccharide of Aerobacter aerogenes.


Hydrolysis of dinucleoside monophosphates containing arabinose in various internucleotide linkages by exonuclease from the venom of Crotalus adamanteus.


The isolation of skeletal muscle mitochondria showing tight coupling, high respiratory indices, and differential adenosine triphosphatase activities.


Insulin-like action of ouabain. I. Effect on carbohydrate metabolism.


Insulin-like action of ouabain. II. Primary antilipolytic effect through inhibition of adenyl cyclase.


Adrenolytic and sympatholytic properties of 2-halogenoalkylamines in the vas deferens of the guinea-pig.


Presence of corticotropin activity in the pituitary gland of chondrichthyean fish.


An improved technique for radial nerve conduction studies.


Granulomatous meningitis and diffuse parenchymatous degeneration of the nervous system due to an intracranial epidermoid cyst.


Membrane impedance changes during synaptic transmission in cat spinal motoneurons.


Dendritic location of synapses and possible mechanisms for the monosynaptic EPSP in motoneurons.


Presynaptic inhibition induced by vagal afferent volleys.


Evaluation of Sabin live poliovirus vaccine in Japan. 3. Studies on the method of administration of Sabin vaccine for babies.


Evaluation of Sabin live poliovirus vaccine in Japan. IV. Marker tests on poliovirus strains recovered from vaccinees and their contacts.


Pituitary corticotrophin reserve and endocrine function in the syndrome of pituitary adenoma with galactorrhea.


Immunofluorescent study on the pathogenesis of oral infection of poliovirus in monkeys.


New vasoconstrictor, bovine peptide-B, released during blood coagulation.


Action of Sendai virus and neuraminidase on the alkaline phosphatase isoenzymes of HeLa cells.


Protein components of encephalomyocarditis virus.


Changes in nucleic acid metabolism of tumour cells under high pressure oxygen.


Carcinogenicity of the herbicide maleic hydrazide.


Hypothalamic and extrahypothalamic mechanisms controlling adrenocorticotrophin secretion.


Hypothalamic control of concomitant secretion of ACTH and TSH.


Excitation and inhibition of ACTH secretion.


Anatomical and functional mapping of central neural input and feedback pathways of the adrenocortical system.


The influence of corticosteroids on the stress-induced release of corticotrophin.


A study of compensatory adrenal hypertrophy and hyperfunction.


Control of adrenal precursors of urinary 17-oxosteroids.


Adrenocorticotrophic activity of synthetic peptide sequences related to ACTH.


Some observations on the control of cortisol secretion in man.


Assessment of pituitary-adrenal function in children.


The effects of prolonged ACTH or corticosteroid therapy in children on growth and on pituitary-adrenal and pituitary function.


The action of histamine, 5-hydroxytryptamine, and blockers upon the 86rubidium kinetics of rat erythrocytes.


Pathological lesions associated with Schistosoma mansoni infection in man.


Enteroviruses in a Trinidad nursery.

Enteroviruses in a Trinidad nursery.


Transformation induced by SV40 in various types of mammalian cell cultures. I. Transformation in cultures of human embryonic cells.


Transformation induced by SV40 in various types of mammalian cell cultures. II. Transformation in hamster, rabbit and mouse kidney cell cultures.


Thermodependence of the growth of attenuated type 1 poliovirus in MS cells.


Prevention of delayed traumatic facial palsy.


Ultrastructural variations of nuclear bodies in human diseases.


Some observations on the ultrastructure of a malignant islet cell tumor associated with duodenal ulceration and severe diarrhea.


Nuclear bodies: a component of cell nuclei in hamster tissues and human tumors.


A glycogen body in liver nuclei.

A glycogen body in liver nuclei.


Comparison of different long-acting corticotropin preparations in the two-day corticotropin test in man.


Quantitative in vitro studies on noradrenaline uptake and its inhibition by amphetamine, desipramine and chlorpromazine.


Sympathetic control of rhythmically active vascular smooth muscle as studied by a nerve-muscle preparation of portal vein.


The effect of DOPA on the spinal cord. 7. Reflex activation of static gamma-motoneurones from the flexor reflex afferents.


Demethylimipramine (desipramine), an alpha-adrenergic blocking agent.


Spread of excitation in the smooth muscle of the rat portal vein.


Comparative effects of carbon tetrachloride and colchicine on xanthine dehydrogenase.


The effect of lithium ions on the post-tetanic potentiation of neuro-muscular transmission.


The role of pyrophosphate and alkaline phosphatase in skin calcification (calciphylaxis).


Quantitative studies on the transmitter release at adrenergic nerve endings.


The effect of DOPA on the spinal cord. 5. Reciprocal organization of pathways transmitting excitatory action to alpha motoneurones of flexors and extensors.


Relationship between DNA synthesis and cell density in normal and virus-transformed cells.


Structural and metabolic distinction between Morris hepatoma 5123 A and normal rat liver.


Dust exposure in mechanical street sweepers.


Tumour-specific antigenicity of aminoazo-dye-induced rat hepatomas.


Hysterosalpingography and pelvic arteriography in postoperative follow-up of hydatidiform mole.


Histochemical studies on two types of alpha-glycerophosphate dehydrogenase in human cervical cancer.


Fluorinated pyrimidines. XXIX. Syntheses of 2',3'-dehydro-5-fluoro-2'-deoxyuridine and 2',3'-dideoxy-5-fluorouridine.


Synthesis and amebicidal activities of some 1',2'-secoemetine derivatives.


2,2'-hydrazobis(5-nitrothiazoles) and analogs, a new type of antiprotozoal agents.


Photochemical degradation of flavins. IV. Studies of the anaerobic photolysis of riboflavin.


l,l-dimethylhydrazine-induced diuresis.

l,l-dimethylhydrazine-induced diuresis.


The suprarenal cortical function as measured by the plasma 17-hydroxycorticosteroid level in malnourished children. I.


Thermostable Clostridium perfringens as the cause of a food poisoning outbreak.


Hormonal influences and salt restriction.


Hospital and community.

Hospital and community.


Effect of incubation temperature on enzyme production for lysine decarboxylase and cytochrome oxidase tests.


Release of a renin-like enzyme from the pregnant uterus of the rabbit.


Purification and properties of methionyl-transfer-ribonucleic acid synthetase from Escherichia coli. 1. Methionyl-t-RNA synthetase (where t-RNA denotes ;soluble' or transfer RNA) has been purified to apparent homogeneity from a ribonuclease I-free strain of Escherichia coli. Polyacrylamide-gel electrophoresis of the final product revealed a single band. The purified enzyme catalyses the exchange of 450mumoles of pyrophosphate into ATP/mg. in 15min. at 37 degrees . 2. Methionyl-t-RNA synthetase is specific for the l-isomer of methionine, but appears to catalyse the methionylation of two distinct species of t-RNA, both of which are specific for methionine, but only one of which may be subsequently formylated. 3. The Michaelis constant for l-methionine is 2x10(-4)m in the ATP-PP(i) exchange assay and 2x10(-5)m for the acylation of t-RNA. 4. Gel filtration of both crude and highly purified preparations of methionyl-t-RNA synthetase on Sephadex G-200 indicates that the active species of enzyme has a molecular weight of about 190000. The amino acid composition of the enzyme is similar to those reported for the isoleucine and tyrosine enzymes from E. coli.


Fluorometric determinations of NADH2 levels in dorsal root ganglion following peripheral nerve stimulation.


Synovioma: review of the literature with case reports.


Isolation and characterization of alkaloids from Caulophyllum thalictroides.


Grayanotoxin. I. Occurrence in additional Ericaceae species.


Segregation kinetics of particulate solids systems. IV. Effect of particle shape on energy requirements.


Hormones and the RES.

Hormones and the RES.


On the conservative treatment of severe ischemia of the leg.


Comparative sequence studies of rat skin and tendon collagen. I. Evidence for incomplete hydroxylation of individual prolyl residues in the normal proteins.


Diphosphopyridine nucleotide specific isocitric dehydrogenase of mammalian mitochondria. II. Kinetic properties of the enzyme of the Ehrlich ascites carcinoma.


Limited trypsin-catalyzed hydrolysis of crystalline human ceruloplasmin. Partial characterization of the digest.


Flavin-pyridinium biscoenzyme analogs. Synthesis and reactivity.


Cholinergic mechanisms in the release of catecholamines from intra-ganglionic inhibitory terminals?


Production of antibodies against synthetic angiotensin II in various animal species.


The substrate supply of the human skeletal muscle at rest, during and after work.


Electron microscopic observations on the sites of activities of succinic dehydrogenase and cytochrome oxidase in Mycobacterium tuberculosis and Bacillus megaterium.


The negative chronotropic effect of adenine derivatives and acetylcholine on frog and rat hearts.


Zollinger-Ellison syndrome.

Zollinger-Ellison syndrome.


Differential studies of angiotensinase activities from several tissue sources.


Effect of cyanide and Antimycin A on the reduction of cytochrome b and ubiquinone in electron transport particles.


Rate of reduction of ubiquinone by NADH in electron transport  particles.


Carrier-free zone electrophoresis of infectious ribonucleic acid derived by phenol and sodium dodecylsulfate methods from purified foot-andmouth disease virus.


Energy-linked reactions in photosynthetic bacteria. I. Succinatelinked ATP-driven NAD reduction by Rhodospirillum rubrum chromatophores.


Interaction of dimethyl-p-phenylenediamine with ceruloplasmin.


Studies on acellular bovine glomeruli. Isolation, chemical composition, and demonstration of collagen with an unusual hydroxylysine: hydroxyproline ratio.


Growth hormone deficiencies in childhood.


Survival after bilateral adrenal hemorrhage during heparin therapy.


[Alveolar cell carcinoma. A report of cases with review of literature].


The specificity of silica cytotoxicity. A study in vitro.


Influence of acute and chronic administration of alcohol on carbohydrate breakdown and energy metabolism in the liver.


In vivo interaction of 4-nitroquinoline-1-oxide and its derivatives with DNA.


Determination of castor oil in heveacrumb rubber by thin-layer chromatography.


Thin layer chromatography of substituted nitroanilines and related compounds.


Chromatoplate contamination removal.

Chromatoplate contamination removal.


An ultrasensitive chemical test for quantitative chromatography of sugars.


Thin-layer chromatography of n-(p-toluenesulfonyl)-carbamates.


Thin-layer chromatographic separation of benefin and related substances.


The quantitative separation of porphyrins and protohaemin as methyl esters by thin-layer chromatography.


Thin-layer chromatography of the peyote alkaloids.


Thin-layer chromatography of vitamin A metabolites in human serum and liver tissue.


Paper and thin-layer electrophoretic separations of polynuclear aza heterocyclic compounds.


Chromatographic separation of 2,4-dinitro-5-aminophenyl derivatives of amino acids.


The identification of colistin and polymyxin B by thin-layer chromatography.


Interaction of phospholipid-metal complexes with water-soluble wheat protein. Insoluble lipid-protein complexes are formed in the presence of Ni(II), Ca(II), or Mg(II) by specific components of the water-soluble proteins of wheat flour and either triphosphoinositide or phosphatidyl serine. The pattern of protein species bound by the lipid-metal complex is dependent upon the metal and the phospholipid used. A group of proteins, containing carbohydrate, may be solubilized and recovered by washing the precipitate with acidic chloroform-methanol-water. Analyses of reactive and nonreactive protein species have shown no differences which clearly account for their behavior. Methylation of protein increases binding to lipid; acetylation decreases the interaction. Weak interaction has been observed between certain components of flour proteins and phospholipid in the absence of metal ions, but the components differ from those bound in the presence of metal ions. It is suggested that properly oriented groups of the protein molecules are chelating onto available coordination positions of metal ions already bound to phospholipid.


Increase in cell lipid and cytoplasmic particles in mammalian cells cultured at reduced pH. The hydrogen ion concentration of the medium has been shown to exert a regulatory effect on the lipid content of cultured mammalian cells. Reduction of the pH of the medium from 7.4 to 6.9 causes a significant increase in cell lipid, relative to cell protein, within 2-3 days. Triglycerides are increased twofold and account for 75% of the additional lipid. Polar lipids, on the other hand, remain nearly constant in concentration. Concurrent with the increase in lipid, particles with an average diameter of 1 micro appear in the cytoplasm. Because the density of these particles is low, ultracentrifugation of the cell homogenate separates the particles completely from the other subcellular structures. The amount of lipid in the particle fraction is approximately equal to the increase in total cell lipid. As shown by silicic acid column chromatography, the particle lipid contains about 75% triglycerides, 15% diglycerides plus an unknown substance, and smaller amounts of material in the monoglyceride and sterol ester-hydrocarbon fractions. The quantitative results indicate that the lipid accumulated at low pH is assembled into discrete cytoplasmic particles.


Lofland HB: Biological activities of vitamins D2 and D3 for growing squirrel monkeys.


Intracranial tumours in rats injected intracerebrally with murine sarcoma virus (MSV), Moloney's strain. Preliminary report.


Activity of cytochrome C oxidase and succindehydrogenase in the lungs in experimental silicosis. I. Early stages.


The measurement of 3beta-hydroxy-delta5 steroids in human fetal blood, amniotic fluid, infant urine and adult urine.


Respiratory virus vaccines. VII. Field evaluation of respiratory syncytial, parainfluenza 1, 2, 3, and Mycoplasma pneumoniae vaccines, 1965 to 1966.


Intrapulmonary chemodectomas in subjects with organizing pulmonary thromboemboli.


Neomycin-polymyxin prophylaxis of urinary-tract infection associated with indwelling catheters.


Micro methods for the quantitative determination of iron and copper in biological material.


Identification of the polymyxins.

Identification of the polymyxins.


Activity of dephosphorylating enzymes in tissue cultures of mouse embryo cells in the course of infection with oncogenic polyoma virus.


Antigonadotropic hormones and lipogenesis in the testis and seminal vesicles of the rat.


Activity of LDH isozymes in the tissue of children with neoplastic diseases.


Template-active RNA isolated from an oncogenic virus.


Natriuretic effect of angiotensin in dogs revealed after administration of reserpine and guanethidine.


Plasma renin activity and renin-substrate concentration in patients with liver disease.


Role of the autonomic nervous system in the renal vasoconstriction response to hemorrhage in the rabbit.


Chemical assay of phospholipid renin preinhibitor in canine and human blood.


Present knowledge of the rhinovirus group of viruses.


A method for studying isolated resistance vessels from rabbit mesentery and brain and their responses to drugs.


Metabolism of C21 steroids by incubated adrenals of ACTH treated rats.


Presynaptic inhibition by gastrocnemius-soleus nerves.


Pressor and oxitocic-like effects of angiotensin affected by aldosterone pretreatment in guinea-pigs.


Renal and pressor actions of angiotensin in salt loaded and depleted rabbits.


Accelerated and prolongated multiplication of antibody-forming spleen cells by Bordetella pertussis in mice immunized with sheep red blood cells.


Avirulent Clostridium perfringens strains obtained by euflavine treatment. Clostridium perfringens was incubated in the presence of euflavine (EU); the resistant mutants which were thus isolated had highly reduced capacity to release alpha-toxin. This fact was confirmed by lecithinase determinations and by immunoelectrophoresis. Injected into guinea pigs and into 6- to 7-day-old chicks, these mutants were completely avirulent. A study of their properties indicated biochemical differences between wild types and mutants.


Antibiotic susceptibility patterns as aids in classification and characterization of gram-negative anaerobic bacilli. Patterns of susceptibility of gram-negative anaerobic bacilli to antibiotics have been found to be distinctive and of significant help in classification and identification. Five major groups of gram-negative anaerobic bacilli have been defined on the basis of morphological and biochemical criteria. Antibiotic sensitivity patterns conform to these groupings and provide additional taxonomic criteria. The Bacteroides fragilis group is resistant to penicillin G, whereas the other groups are generally sensitive. B. fragilis strains are relatively sensitive to erythromycin, whereas the Sphaerophorus necrophorus group is resistant. B. melaninogenicus strains, B. oralis, and Fusobacterium are all more sensitive to kanamycin and neomycin than the other two groups. Kanamycin is more active against Fusobacterium strains than neomycin, but less active against all other groups. Colistin or polymyxin B is useful for distinguishing between the resistant B. fragilis and the sensitive S. necrophorus. Antibiotic susceptibility determinations may be more readily performed in clinical laboratories than certain biochemical tests recommended for differentiation of the gram-negative anaerobic bacilli and may serve as helpful adjuncts to morphological and biochemical observations in classifying and characterizing these organisms. The use of standardized procedures for antibiotic susceptibility tests is essential if comparable results are to be obtained in different laboratories.


Plasma testosterone in idiopathic hirsutism, and the changes produced by adrenal and ovarian stimulation and suppression.


The effect of norethandrolone (Nilevar) on growth in the female guinea-pig.


Dynamic aspects of collagen biology. II. Degradation and metabolism.


Metastatic renal embryoma: survival following therapy.


Acute leukemia and infection.

Acute leukemia and infection.


Bites by copperheads (Ancistrodon contortrix) in the United States.


Fatal nonbacterial pneumonia associated with Adenovirus type 4. Occurrence in an adult.


Renal pressor mechanisms.

Renal pressor mechanisms.


Effect of berberine sulphate on Entamoeba histolytica.


Agent of Marek's disease in tissue culture.


Effect of suspending medium on heat resistance of spores of Clostridium perfringens.


Inorganic pyrophosphate and ATP as energy donors in chromatophores from Rhodospirillum rubrum.


Influence of host age on leukaemogenesis and virus proliferation after infection with a murine leukaemia virus.


Cytopathic effects of the parainfluenza virus SV5 in Vero cells.


Lysosomal enzymes in relation to the toxicity of silica.


Transmission of excitation from the parasympathetic nerve to the smooth muscle.


Observations on the relationship between quartz cytotoxicity and fibrogenicity while testing the biological activity of synthetic polymers.


Granular-cell myoblastoma of tongue. Report of a case.


Diabetes mellitus and infantilism.

Diabetes mellitus and infantilism.


A new type 3 attenuated poliovirus for possible use in oral poliovirus vaccine.


The cat-scratch syndrome, many diseases or one disease?


Comparative virology of the herpes group.


In vitro investigations on murine leukemia viruses.


Detergent-solubilized RNA polymerase from cells infected with foot-and-mouth disease virus. The foot-and-mouth disease virus RNA polymerase complex was dissociated from cellular membranes with deoxycholate in the presence of dextran sulfate. The soluble polymerase complex was active in the cell-free synthesis of virus-specific RNA; solubilization of the complex permitted direct analysis of the cell-free reaction mixtures without recourse to RNA extraction. A major RNA-containing component found early during cell-free incubation ranged from approximately 140 to 300S. The final major products of the cell-free system were 37S virus RNA, 20S ribonuclease-resistant RNA, and a 50S component containing RNA.


Steroid reactions on thin-layer films.

Steroid reactions on thin-layer films.


Effect of total gastrectomy on the Zollinger-Ellison tumor: observations by second-look procedures.


Treatment of clostridial infections with hyperbaric oxygen.


The susceptibility of mycobacteria to rifamide and rifampicin.


Analysis of labeling patterns in soluble RNA preparations from green pea stem sections supplied with 14C-carboxyl-labeled indoleacetic acid.


Studies on characteristics of poliovirus. I. Marker and temperature.


The existence of two different metabolic patterns for the synthesis of RNA viruses.


A new virus cultivated only in organ cultures of human ciliated epithelium.


Further properties of equine arteritis virus.


Copper sulfate-induced hemolytic anemia. Inhibition of glucose-6-phosphate dehydrogenase and other possible etiologic mechanisms.


Cytopathic effect and growth of SV 40 in rhesus monkey kidney cell cultures.


The inhibition of NADH oxidation in mammalian mitochondria by chloramphenicol.


Polyriboadenylate synthesis by nuclei from developing sea urchin embryos. I. Characterization of the ATP polymerase reaction.


Central respiratory failure reversed by treatment.


Current approaches to the mechanism of energy-coupling in the respiratory chain. Studies with yeast mitochondria.


Surgical management of tumors of intraoral minor salivary glands. Report of eighty cases.


Classification of primary malignant tumors of liver in infancy and childhood.


Studies on P-450. II. On the absorption spectrum of P-450 in mitochondrial fragments from bovine adrenal cortex.


Reactive amino groups in baker's yeast cytochrome C.


Study of a virus-containing hematopoietic cell line and a melanoma cell line derived from a patient with a leukemoid reaction.


The effects of sodium and angiotensin upon renal renin and renal, adrenal, and salivary gland glucose-6-phosphate dehydrogenase. Evidence for a proximal tubular effect of angiotensin.


Phenomenon of tumour transfer by skin grafting.


Recognition of the individuality of tumour strain by sensitized peritoneal lymphoid cells.


Increased tumour induction by adenovirus type 12 in thymectomized mice and mice treated with anti-lymphocyte serum.


Immunological reactivity to peptides related to angiotensin.


Heat-labile mcaroglobulin revealed in adenovirus 12 tumour sera by gel electrophoresis.


Relationship between the subunits and "T" antigens of adenovirus type 12.


Multinucleated muscle fibres: induction of DNA synthesis and mitosis by polyoma virus infection.


Parathyroid function and the renal excretion of 3'5'-adenylic acid.


Further studies on cytomegalovirus mononucleosis in previously healthy individuals.


Fixation of the annulus of the mitral valve with a rigid ring. Hemodynamic studies.


The epizootiology of the vesicular stomatitis viruses: a reappraisal.


The nature of adrenocorticosteroid activity.


Immunization with live type 4 adenovirus: determination of infectious virus dose and protective effect of enteric infection.


The sensitivity of the complement fixation test for the detection of adenovirus infections in infants and children with lower respiratory disease.


Epidemiology of infections with rhinovirus types 43 and 55 in a group of university of Wisconsin student families.


Contrast nasopharyngography of angiofibromata.


Erythrocytosis and hyperlipemia as manifestations of hepatic carcinoma.


Experimental infection with Streptomyces madurae as a function of collagenase.


Lysis of clots in serum by proteolytic enzymes other than trypsin and plasmin.


A neurological disorder preceding Hodgkin's sarcoma.


Therapy of infective gastroenteritis.

Therapy of infective gastroenteritis.


Electron spin resonance studies on proton-irradiated ribonuclease and lysozyme.


Inactivation and trapped radicals in dry trypsin exposed to ultraviolet light.


Ultraviolet-induced excited states in deoxyribonucleic acid.


Free radicals induced in enzymes by electrons and heavy ions.


Cis-trans isomerism in naphthoquinones: interconversion and participation in oxidative phosphorylation. Synthetic phylloquinone was resolved into cis and trans isomers by thin-layer chromatography. The two isomers had identical ultraviolet spectra characteristic of vitamin K(1) and were differentiated by nuclear-magnetic-resonance spectroscopy on the basis of the displacement of the peak corresponding to the olefinic methyl group in the naphthoquinone side chain. Studies on the restoration of electron transport coupled to phosphorylation in irradiated preparations of Mycobacterium phlei showed that only the trans isomer was active with substrates linked to nicotinamide-adenine dinucleotide. The purified trans phylloquinone was enzymatically converted to the cis isomer. Under similar conditions, cis vitamin K(1) gave rise to the trans-naphthoquinone. The natural naphthoquinone of M. phlei vitamin MK(9)(II-H) was similarly resolved into cis and trans isomers.


Control of aldosterone secretion by the pituitary gland. In the rat, the pituitary gland is essential for the stimulation of aldosterone secretion by sodium depletion. Hypophysectomy abolishes the response to sodium depletion, whereas whole pituitary gland injections partially restore it. The response cannot be restored by injections of either adrenocorticotropin or growth hormone, nor by adrenocorticotropin plus thyroxin. The pituitary gland must secrete a hormone or possibly several hormones which are necessary for the adrenal gland to respond to sodium depletion.


Extrahepatic biliary decompression in traumatized canine livers.