Regulation of DNA synthesis in fat cells and stromal elements from rat adipose tissue. The incorporation of tritiated thymidine into the deoxyribonucleic acid (DNA) of adipose fat and stromal cells was followed under a variety of conditions. After in vitro incubation of adipose slices or up to 2 days after in vivo injection of the isotope, all DNA radioactivity was in the stromal cell fraction. From 2 to 15 days after thymidine injection total tissue DNA radioactivity was constant, while between 2 and 5 days after injection label in fat cell DNA increased markedly. Thus new labeled fat cells, initially collected in the stromal pool, required 2-5 days after completion of DNA synthesis to accumulate sufficient lipid to be harvested in the fat cell fraction. Fasting before thymidine injection practically abolished DNA synthesis in primordial fat cells and reduced less drastically formation of stromal elements. However fasting sufficient to deplete lipid stores by 50% neither destroyed mature fat cells nor impaired their capacity to reaccumulate fat with refeeding. Other studies evaluated the role of new fat cell formation in the process of lipid accretion accompanying refeeding. These experiments indicated that at least during the early phase of rapid weight gain, accumulation of fat was due to deposition of triglyceride in existing cells rather than to accelerated formation of new fat cells. Studies with hypophysectomized rats demonstrated that pituitary ablation variably affected stromal DNA synthesis and nearly abolished the formation and (or) maturation of primordial fat cells. In these animals growth hormone markedly enhanced thymidine incorporation into stromal DNA but had no effect on fat cell precursors. In intact animals the predominant effect of growth hormone was also on the stromal fraction, although an action of the hormone of lesser magnitude on fat cell precursors was also evident.


Metabolism and rate of secretion of aldosterone in the bullfrog. A study of the metabolism of aldosterone in the bullfrog was undertaken to provide a measurable metabolite for the indirect isotope dilution technique for measuring secretory rates. The rate of excretion of labeled aldosterone was considerably slower in the frog than in man and made necessary the collection of excretory products for 5 days to insure reasonably complete recovery of metabolites. The major identifiable metabolite was a tetrahydro derivative subsequently identified as 3beta-hydroxy-5beta-tetrahydroaldosterone. This metabolite was excreted partly in the free form and partly as a glucuronic acid conjugate. The pH 1-hydrolyzable conjugate of aldosterone was not detected. For the measurement of secretory rates, aldosterone-(3)H was injected into the dorsal lymph space and the animal placed in a bath to provide an environment of constant electrolyte composition and intake and a means of collecting excretory products. Urine and bath fluid were collected for 5 days, tetrahydroaldosterone was isolated, and its specific activity determined for the calculation of the aldosterone secretory rate. The rate of secretion of aldosterone in the bullfrog was increased by a tap water bath and by bovine adrenocorticotropic hormone, decreased by a saline bath and by dexamethasone, and unchanged by valine-5-angiotensin amide.


The activity of enzymes in the rabbit uterus and effect of progesterone and oestradiol.


Lack of effect of dexamethasone on the adrenocortical response to corticotrophin in sheep with adrenal autotransplants.


Lipolysis in chicken adipose tissue in vitro.


Building on a bone; or how Dr. Krogman parlays skeletal elements into diverse careers.


Thyroxine-induced acute enhancement of pituitary-adrenal function in the rat.


Failure of corticosterone to suppress pituitary ACTH release in rats exposed to various noxious stimuli.


Mucinous adenocarcinoma of the urachus.

Mucinous adenocarcinoma of the urachus.


Fat embolism: an experimental study (II). Evaluation of smooth muscle relaxants in its treatment.


Cell culture techniques for primary isolation of Marek's disease-associated herpesvirus.


The morphologic response of the turkey oviduct to certain pathogenic agents.


Pleliminary observations on possible synergism between infectious laryngotracheitis virus and hemophilus gallinarum.


Evidence for a herpesvirus as an etiologic agent of marek's disease.


Transmission of classical Marek's disease by affected and carrier birds.


Early changes in bursa of fabricius from Marek's disease.


Studies on an ocular infectious laryngotracheitis vaccine.


Owl pox inclusion-body formation in skin epithelium of chicks.


Avian leukosis-sarcoma viruses and antibody in field flocks, and their relationship to "leukosis" mortality.


Radioautographic and radiochemical analysis of postnatal collagen formation in mouse dermal skin.


Polyneuritis.

Polyneuritis.


Induction of sexual excitement with intraventricular ACTH; permissive role of testosterone in the male rabbit.


Working hypothesis: viral etiology of autoimmune diseases.


'Granular-cell myoblastoma' in the horse. A report of 4 cases.


Blood cells and chemistry of young chickens during daily ACTH and cortisol administration.


Effect of phytate on the calcium requirement of chicks.


The availability of phytate phosphorus in soybean meal before and after treatment with a mold phytase.


Radiosensitivity and biological properties of tumors. XIV. Correlation between mitochondrial structure, oxidative metabolism, and radiosensitivity.


Prevention by arginine glutamate of the carcinogenicity of acetamide in rats.


Altered sensitivity to drugs following repeated injections of a cholinesterase inhibitor to rats.


An autopsy case of islet-cell carcinoma with Cushing's syndrome.


Role of host factors in the induction of leukemia by radiation and virus.


Effects of bleomycin on transplantable mouse tumors.


Antiviral activity of mycophenolic acid. Studies on antiviral and antitumor antibiotics. IV.


Further studies of the infectivity and stability of extracts and homogenates derived from scrapie affected mouse brains.


The use of bacteriocidal agents in the primary isolation of Mycoplasma mycoides.


Foot-and-mouth disease in wildlife in Rhodesia and other African territories. A serological survey.


The isolation of adenoviruses and enteroviruses from pig tissues.


Carcinogenesis in tissue culture. 8. Spontaneous malignant transformation of rat liver cells in long-term culture.


Lipids of cancer tissues. IV. Glycolipids of human gastric carcinoma.


Chemical structure of the main glycolipid of hog erythrocytes.


On the glucocerebroside in the brain of old patients.


Effect of ultraviolet light on the epidermis. 3. Histochemistry of cell respiration and phosphorylation.


Ectopic inhibition of the complexes of the electron transport system by rotenone, piericidin A, demerol and antimycin A.


Serum "Angiotensinase activity" and female steroidal hormones.


Activation Ca2+ uptake by acetyl phosphate in muscle microsomes.


Site-specific uncoupling and inhibition of oxidative phosphorylation by biguanides. II.


Detection by immunodiffusion of mouse mammary tumor virus in milk samples and correlation with tumor development.


An ultrastructural study of the toxic and carcinogenic effects of 2-amino-5-azotoluene on the livers of schistosome-infected and uninfected mice.


The effect of phleomycin on the replication of papovavirus SV40 and other DNA viruses in simian cells.


Profiles of total ribonucleoprotein particles from normal rat liver, primary liver tumors, and Novikoff hepatoma.


Ultrastructural modifications of the cell surface and intercellular contacts of some transformed cell strains.


Chromosomes of "minimal deviation" hepatomas: a further report on diploid tumors.


Protection against mammary tumors in mice by immunization with purified mammary tumor virus preparations.


Effect of ethanol exposure during embryogenesis and the neonatal period on the incidence of hepatoma in C3H male mice.


Delays in arranging aftercare.

Delays in arranging aftercare.


Family group counseling.

Family group counseling.


Inhibitory effect of lysolecithin on electron transfer from NADH to cytochrome b.


Plasma membranes of the rat liver. Isolation and enzymatic characterization of a fraction rich in bile canaliculi. A method is described for the rapid isolation of a plasma membrane fraction containing a high concentration of intact bile canaliculi from the rat liver. Isolated bile canaliculi retain most of the ultrastructural features exhibited in the intact liver cell. The final fraction contains 5'-nucleotidase activity at approximately the same concentration as that in previous preparations of plasma membranes. In the presence of 0.01 M Mg(++), 5'-nucleotidase exhibits a double pH optimum at pH values of 7.5 and 9.5. The activities of glucose-6-phosphatase and alkaline phosphatase are present in low amounts. Cytochrome P-450 is not detectable. Na(+)-K(+)-activation of ATPase is observed to the extent of 20-36% in about half of the assays. The availability of a method for preparation of intact bile canaliculi should prove useful for studying the biochemical events associated with the transport of bile constituents into canaliculi.


Morphogenetic aspects of multilayering in Petri dish cultures of human fetal lung fibroblasts. Randomly seeded Petri dish cultures of embryonic human lung fibroblasts generate, in the course of their growth, highly ordered cellular arrangements. Thick, bilaterally symmetrical ridges with an axial polarity and an orthogonal, multilayered internal organization are observed within stationary cultures. The generation of these structures has been investigated. Ridges result from the spontaneous aggregation of cells in postconfluent cultures brought about by directed cell movements. These movements are promoted by the localized production of extracellular matrix sheets containing collagen, which provide new substrates for cellular colonization. Cells that have colonized one matrix substrate may secrete another above themselves, which will in turn be colonized. By a continuation of this cycle, thick stacks consisting of alternate layers of cells and matrix are produced to yield the observed aggregations. The distribution and shape of ridges in a culture imply that matrix substrates are confined to specific locations. The suggested control hypothesis assumes that all the cells in fibroblast cultures are potential producers of a single species of matrix. The serviceability of this matrix as a substrate for cellular colonization, however, is destroyed if the producer cells are motile. Matrix substrates, therefore, are only made by nonmotile cells.


Ultrastructural and biochemical changes in brown fat in cold-exposed rats. During the first 3 days of exposure of rats to 5 degrees C, the nitrogen concentration of interscapular brown fat increased by 50% and remained at this elevated level for the duration of the 8-wk observation period, while the mass of tissue increased fourfold. The concentration of both DNA and RNA per unit nitrogen reached a maximum after 3 days, then declined; however, the total quantity of each continued to rise. The concentration of various respiratory enzymes decreased during the first few days and then increased, but at different rates. The morphological changes in mature brown fat cells during cold acclimation were observed to be: a reduction in fat droplet size during the first 3 days, followed by a gradual increase in size through 6 wk in the cold; a continual increase in the amount of intermitochondrial ground substance during the first 3 wk, with increased granularity and glycogen content after 1 wk; initial disappearance of glycogen between mitochondria, followed by the reappearance of a few isolated particles in the intermitochondrial ground substance after 1 wk in the cold; initial increase in the density of intramitochondrial matrix for the first 3-4 days, followed by a gradual return to the control density; loss in integrity of mitochondrial outer membranes during the first 4 days, followed by gradual but incomplete restoration; temporary loss of the dense material in lipid droplets during the first 24 hr, with return after 1 wk in the cold; and a 40% increase in mitochondrial diameter within 1 day, followed by a decrease in diameter within 1 wk to a constant value about 15% larger than the controls.


Incorporation of radioactivity from 14C-sugars into macromolecules in poliovirus-infected or guanidine-treated HeLa cells.


Incorporation of lipids into herpes simplex virus particles.


A metastatic Wilm's tumour in the nasopharynx.


Results of a comparison of serologic tests used to detect human antibodies to a herpesvirus (HLV) associated with Burkitt's lymphoma.


Growth in vitro and ultrastructure of cells from a medullary carcinoma of the human thyroid gland: transformation by simian virus 40 and evidenc of thyrocalcitonin and prostaglandins. Continuous single layer tissue cultures were established with explants from a metastatic nodule of medullary carcinoma primary in the thyroid gland.  Some cultures were infected with simian virus 40 and the cells acquired morphologic and growth characteristics associated with viral transformation.  Thyrocalcitonin activity was detected by radioimmunoassay of culture fluids as late as 7 months after initiation of the single layers.  Substances with the chemical and biological properties of (PG) prostaglandins E2 and F2alpha were extracted from culture fluids after 4 months.  The original carcinoma cells contained abundant cyctoplasmic secretory granules, which were demonstrated by electron microscopy.  These granules disappeared during subculture of the monolayers, and electron-dense secretory substance accumulated within dilated cisternae of the ribosome-bound endoplasmic reticulum.  Ultrastructural differences in surface organization of the virus transformed and uninfected culture cells were noted.  Amyloid was present in the original tumor stroma and tissue culture explants, but could not be identified in those serial subcultures derived from the monolayer outgrowths.


Antiviral effect of daunomycin.

Antiviral effect of daunomycin.


Auditorially evoked responses. A tool for assessing the patient's ability to hear during various states of consciousness.


Fibrinogen response to corticotropin preparations in rabbits.


Adrenal steroidogenesis and serum renin in rats bred for juxtaglomerular granularity.


Mechanism of neuromuscular blockade by tetraethylammonium.


Characterization of bacteria by gas chromatography: comparison of trimethylsilyl derivatives of whole-cell hydrolysates. A method for the formation of the trimethylsilyl (TMS) derivatives of the whole-cell hydrolysate of bacteria was developed. The TMS derivatives were examined by gas-liquid chromatography. TMS profiles of various bacteria at the genus and species level were compared. Differences in TMS profiles of Listeria, Neisseria, and Clostridium were significant as were differences between the TMS profiles of C. perfringens and C. sporogenes. Two types of C. perfringens, two serotypes of L. monocytogenes, and one culture of C. sporogenes and N. meningitidis were studied. The possible application of TMS profiles as an aid in differentiating closely related organisms which are troublesome to separate by conventional means is discussed.


Antibiotic additives for the isolation of Chondrococcus columnaris from fish.


Sporulation, heat resistance, and biological properties of Clostridium perfringens. A sporulation medium for 134 Clostridium perfringens strains, including types A, B, C, D, E, and F, was devised according to Grelet's observation that sporulation occurred when cultural environment became limited in any nutritional requirement indispensable for the growth of the organism. Sporulation took place most prominently when 10% cooked-meat broth (pH 7.2) containing 3% Proteose Peptone and 1% glucose was used for the preculture and 2% Poli Peptone medium (pH 7.8) was used for the subculture medium. Sometimes, terminal spores could be observed. A correlation between sporulation and heat resistance was examined by use of C. perfringens strains isolated from samples heated at different temperatures. Almost all strains isolated from unheated samples and from those heated at lower temperatures gave rise to spores in our sporulation medium, but the spores were weakly heat-resistant, whereas strains isolated from samples heated at 100 C for 60 min were highly heat-resistant but sporulated poorly. A majority of these heat-resistant strains were non-gelatinolytic and definitely salicin-fermenting.


Influence of adrenal cortical hormones on the norepinephrine-induced lipolysis.


Specificity of the dehydrogenases of maize endosperm.


Plasma membranes of liver cells of the chick embryo. I. Isolation procedures.


Effects of ATP and Ca2+ on a K+-activated phosphatase from red blood cell membranes.


Alpha-aminoisobutyric acid uptake in isolated mouse fat cells.


A spin-label study of erythrocyte membranes.


The stepwise acceleration of phosphatidyl choline synthesis in phytohemagglutinin-treated lymphocytes.


The chromatographic separation of phosphonolipids from their phospholipid analogs.


The in vitro degradation of dihydrosphingosine.


Isomerization of 5-androsten-3,17-dione by hepatic tissue.


Steroid estrogen conjugates of hen's urine: formation in vivo of steroid estrogen monosulphates and disulphates from injected estradiol-17-beta.


Histologic changes in incipient carcinoma of the breast.


Endocrine influences on human mammary cancer formation. A critique.


Team approach tthe study of diseases of the breast.


Genital herpetic infection. Association with cervical dysplasia and carcinoma.


Urinary testosterone production and metabolism in man: sequential effects of dexamethasone, estrogen, ACTH and HCG.


The effect of thyroxine and cortisol on some dehydrogenases and lysosomal enzymes activities in rat pineal cultures.


Histochemistry of the pancreatic islets in golden hanster Mesocricetus auratus Waterhouse 1839.


Nonequilibrium alpha adrenergic blockade and neuroleptic activity of N-ethoxycarbonyl-1,dihydroquinoline (BC-347).


Adrenalectomy in cases of severe renal sclerosis.


Granular-cell myoblastoma of the vulva.

Granular-cell myoblastoma of the vulva.


Malignant mixed mullerian tumors of the uterus. A clinicopathologic study of 9 patients.


Anaesthesia in patients with disturbances of cerebral and coronary circulation.


The distribution of alkaline glycerophosphatase in the embryonic, regenerative, and normal mature tails of Hemidactylus bowringi (Gray).


Selective celiac angiography with special reference to carcinoma of the liver.


Conjugated estrogenic substances.

Conjugated estrogenic substances.


Effects of reovirus type 1 on the developing mouse embryo.


Analytical applications of the phosphatase enzyme system. Determination of bismuth, beryllium and pesticides.


Determination of mercury residues in potatoes, grain and animal tissues using perchloric acid digestion.


Genetic and epigenetic control of several isozymic systems in Tetrahymena.


Genetic and epigenetic forms of malate dehydrogenase in Neurospora.


Significance of substrate inhibition of dehydrogenases.


Drosophila alcohol dehydrogenase: in vitro changes of isozyme patterns.


Protection of lactate dehydrogenase isozymes from heat inactivation and enzymatic degradation.


An ESR study of short-lived radicals derived from pyrimidines in aqueous solution.


Ribonucleic acid synthesis in relation to precursor pools in regenerating rat liver.


Studies on ribonucleic acid synthesis in nuclei isolated from rat liver.


Symptomatic optic neuritis (etiological factors in acute optic neuritis).


Psychological factors and prognosis in peripheral neuropathy.


The experimenter variable in dream research.


Sleep patterns and EEG recordings in twin idiot savants.


The effect of vitamin A deficiency on myelination in the central nervous system of the rat.


The effect of antirenin on the blood pressure of the rat with exprimental renal hypertension.


On lumpers and splitters, or the nosology of genetic disease.


Neuropathology.

Neuropathology.


Peripheral nerve surgery.

Peripheral nerve surgery.


Pharmacology of the central nervous system.


Neuro-endocrine relationships.

Neuro-endocrine relationships.


The neuroses.

The neuroses.


Effects of anticonvulsant drugs upon patterns of seizure discharge and brain thresholds: some implications for memory mechanisms.


The after-care of the patient in the x-ray department.


Gynecology and obstetrics.

Gynecology and obstetrics.


Observations on distribution of NADH oxidase in particles from dark-grown and light-grown Rhodospirillum rubrum.


An EPR signal generated by the ethanolamine deaminase-coenzyme B 12 complex in the presence of substrate.


Epinephrine induced formation of adenosine 3', 5'-monophosphate in mouse skeletal muscle.


Clinical and pathologic observations after orthotopic transplantation of the human liver.


Effect of cytosine arabinoside 5'-triphosphate on mammalian DNA polymerase.


Chromosomal evolution of a secondary clone from the SV40 transformed human cell line, W18 Va2.


Evidence for direct hydrogen transfer during glyceraldehyde-3-phosphate dehydrogenase catalysis.


Nucleotide sequence studies with ribonucleic acids from liver and hepatoma of the rat using DNA-RNA hybridization.


The effect of esteroproteases from mouse submaxillary gland on the growth of rat hepatoma cells in tissue culture.


The relative nephrotoxicities of polymyxin B sulfate, sodium sulfomethyl-polymyxin B, sodium sulfomethyl-colistin (colymycin), and neomycin sulfate.


D-mannitol metabolism by Aspergillus candidus. Pathways of mannitol biosynthesis and utilization in Aspergillus candidus NRRL 305 were studied in cell-free extracts of washed mycelia prepared by sonic and French pressure cell treatments. A nicotinamide adenine dinucleotide-linked mannitol-1-phosphate (M1P) dehydrogenase was found in French pressure cell extracts of d-glucose-grown cells, whereas a specific mannitol-1-phosphatase was present in extracts prepared by both methods. The existence of these two enzymes indicated that mannitol may be synthesized in this organism by the reduction of fructose-6-phosphate. A specific nicotinamide adenine dinucleotide phosphate-linked mannitol dehydrogenase was also identified in both extracts. This enzyme may have been involved in mannitol utilization. However, the level of the mannitol dehydrogenase appeared to be substantially reduced in extracts from mannitol-grown cells, whereas the level of M1P dehydrogenase was increased. A hexokinase has been identified in this organism. Fructose-6-phosphatase, glucose isomerase, and mannitol kinase could not be demonstrated.


The development of patterened activity by implanted ganglia and their peripheral connexions in Periplaneta americana.


Excitation and habituation of the crayfish escape reflex: the depolarizing response in lateral giant fibres of the isolated abdomen.


The synaptic link between the sensory and motoneurones in the eye-withdrawal reflex of the crab.


Deprivation of paradoxical sleep and brain glycogen.


Case for diagnosis.

Case for diagnosis.


New light-sensitive cofactor required for oxidation of succinate by Mycobacterium phlei. Irradiation of the electron transport particles of Mycobacterium phlei with light at a wavelength of 360 manometers resulted in a loss of oxidase activities of succinate and the reduced form of nicotinamide adenine dinucleotide. The lesion in the two pathways caused by irradiation of the particles differs. The succinoxidase pathway was more labile to irradiation than the pathway linked to nicotinamide adenine dinucleotide. Restoration of succinoxidase activity (up to 50 to 60 percent) occurred on addition of a thermostable, water-soluble material obtained from Mycobacterium phlei cells or with an extract of mitochondria from boiled rat liver. Other known cofactors, such as flavine adenine dinucleotide, flavine mononucleotide, benzo- and naphthoquinones, as well as sulfhydryl agents, failed to restore succinoxidase activity after irradiation. Water-soluble material from Mycobacterium phlei appears to function between the flavoprotein and cytochrome b on the succinoxidase pathway. In contrast to the requirements for restoration of the pathway linked to nicotinamide adenine dinucleotide, restoration of succinoxidase does not occur with quinones or other cofactors such as flavine adenine dinucleotide.


Molecular order of participation of inhibitors (or activators) in biological systems. From a Hill plot it is frequently possible to determine whether exactly one, two, or several inhibitor (or activator) molecules are involved in a critical rate-determining biological process.


The early effects of experimental exposure, manipulation, and temporary ischaemia on the rabbit kidney. A histological and biochemical study.


Biochemistry of hypoxia: current concepts. II. Biochemical derangements associated with hypoxia and their measurement.


Osteoclastoma of the petrous temporal bone.


Adrenal oxidative enzymes in ox and sheep. The histochemical demonstration of some pathways involved in chromaffin granule synthesis.


Mottled areas of intra-abdominal calcification.


Freezing injury in relation to loss of enzyme activities and protection against freezing.


Experience with a rapid oral metyrapone test and the plasma ACTH content in determining the cause of Cushing's syndrome.


Plasma glucose response to natural vasopressin in maturity onset diabetics and adult nondiabetics.


Stability of purified foot and mouth disease virus after freeze-drying and storage.


The mechanism of aluminium in the prevention of pneumoconiosis.


Effects of sucrose and dactinomycin on hepatic acid hydrolases.


The one-hour synthetic corticotrophin test for rapid assessment of adrenal function.


Use of intravenous vitamin D3 in the detection of vitamin D deficiency.


On the occurrence of neoplasms in domestic fowl--an angiosarcoma and a malignant hepatoma.


Cystic abdominal tumor in a 66 year old man.


Amino acid activation and formation of amino acyl s-RNA in bovine parotid gland.


Subacute denervation: a means of disclosing mammalian motor nerve terminals as critical sites of acetylcholine and facilitatory drug actions.


The proposed ACGIH mass limits for quartz: review and evaluation.


Investigations on a series of heterocyclic hemicholinium-3 analogs.


Neurogenic basis for the rise in blood pressure evoked by nicotine in the cat.


Subcellular localization of monoamine oxidase in rat tissues.


Effect of cold on plasma protein-thyroxine interaction in the guinea pig.


Microstructure of "lypochondria" from cultured cells.


Cell physiology. Essential element.

Cell physiology. Essential element.


Temperature dependence of the EPR signal in tendon collagen.


Detection and investigation of a new type of ESR signal characteristic of some tumour tissues.


New vitamin D metabolite localized in intestinal cell nuclei.


Curve-fitting of allosteric binding data.


Facilitation of peptide synthesis by the use of 4-picolyl esters: synthesis of va15-angiotensin II.


Susceptibility of the domestic pig to influenza B virus.


Significance of a complement-fixing antigen associated with herpes-like virus and detected in the Raji cell line.


Ependymomas induced in hamsters inoculated with an avian adenovirus (CELO).


Israel.

Israel.


The histographic analysis of human muscle biopsies with regard to fiber types. 2. Diseases of the upper and lower motor neuron.


Clinical laboratory experiences with a more attenuated Enders' measles virus vaccine (Moraten) combined with smallpox vaccine.


Photoprocesses and molecular associations.


Actions of sympathomimetic amines and their antagonists on skeletal muscle.


Some electron spin resonance characteristics of human female breast tumours.


Drug addicts in a therapeutic community. Outline on the California Rehabilitation Center Program, Corona.


A treatment strategy of dealing with an entrenched long hospitalized patient group.


Psychiatric aftercare in a general hospital. A system of prevention in the community.


Changes in drinking behavior in rats in response to large doses of alcohol.


Retinal processing of visual images.

Retinal processing of visual images.


Hydroxamic acids in nature.

Hydroxamic acids in nature. The hydroxamic acid bond occurs in products from fungi, yeast, bacteria, and plants. The -CON(OH)-bond arises by oxidation of a free or bound amino group in a unit structure which is often closely related to conventional amino acids. Products are known with one, two, or three hydroxamic acid groups per molecule. The chemistry of the ferrichrome type compounds, which are ferric trihydroxamate-containing peptides, has been worked out in detail and includes a complete crystallographic analysis of the ferrichrome A molecule. The trihydroxamates form potent complexes with ferric ion, called siderochromes, and these are believed to play a role in the metabolism of the metal ion in microorganisms. The actual physiological activity observed ranges from that of growth factor, antibiotic, antibiotic antagonist, tumor inhibitor or cell-division factor. The precise molecular mechanism whereby these substances exert their potent beological activity remains to be elucidated.


Selective release of enzymes from bacteria. A group of hydrolytic enzymes, including phosphatases and nucleases, is selectively released from E. coli and certain other Gram-negative bacteria by a process designated as osmotic shock. This procedure involves exposure of the cells to ethylenediaminetetraacetate (EDTA) in 0.5 molar sucrose followed by a sudden osmotic transition to cold, dilute MgCl(2). Osmotic shock also results in an alteration of the permeability barrier of the bacterial cell and a depletion of the pool of acid-soluble nucleotides, but there is no loss of viability. On being restored to growth medium, the shocked cells recover after a lag period. Formation of spheroplasts by treatment with EDTA and lysozyme leads to selective release of the same group of enzymes. We believe that the selectively released enzymes are confined in a region between the bacterial cell wall and the cytoplasmic membrane. Histochemical studies indicate such a localization. Further, the enzyme activities are measurable with intact cells, even when the substrate is a nucleotide, to which whole cells are impermeable. Another piece of evidence concerns a mutant E. coli with a defective cell wall. In contrast to normal bacteria, this organism loses one of these enzymes into the medium in the course of growth. After osmotic shock, the bacteria show reduced uptake of sulfate,betagalactosides, galactose, and certain amino acids. Furthermore, the shock treatment causes the release of nondialyzable factors able to bind sulfate, galactose, and the same amino acids. A possible interpretation of these observations is the following: the binding proteins occupy sites near the bacterial surface, and they may be components of active transport systems responsible for the concentrative uptake of these nutrients.


Dormin (Abscisin II), inhibitor of plant DNA synthesis? Dormin (abscisin II), inhibits growth of Lemna minor cultures. At 1 part per million (3.8x10(-6)M), the culture appears nearly completely dormant but can be revived readily by transferring it to fresh medium free of dormin. The cytokinin benzyladenine, but not auxin or gibberellin, will counteract the dormin effect. Quantitative restoration of normal growth by cytokinin, however, can be achieved only if the dormin concentration does not exceed a critical level. Separation, after phenol-detergent extraction, of nucleic acids on methylated albumin kieselguhr columns showed suppression of nucleic acid synthesis by dormin in all fractions. Inhibition of the synthesis of the DNA fraction seems to precede that of RNA. Cytokinins reverse the process. They promote synthesis of all nucleic acid fractions, but again DNA seems to lead. Further work on the interaction of dormin with growth-promoting hormones might be facilitated by adopting the Monod model of allosteric transition, with, for example, DNA polymerase as the protein, dormin as the inhibitor, and cytokinin or other growth promoters as activators.


Primordial germ cells in blood smears from chick embryos. The feasibility of studying avian primordial germ cells in blood smears has been demonstrated. Blood smears prepared from chick embryos of stages 13 to 15 (48 to 55 hours) contained primordial germ cells, which were revealed by the periodic acidSchiff reaction. The presence of glycogen in the cytoplasm of the primordial germ cells facilitated their selective identification.


"Galactose dehydrogenase", "nothing dehydrogenase," and alcohol dehydrogenase: interrelation.


"Galactose dehydrogenase", "nothing dehydrogenase", and alcohol dehydrogenase: interrelation.


Nature and mechanisms of oxygenases.

Nature and mechanisms of oxygenases.


Regulation of the adrenal cortex secretory pattern by adrenocorticotropin. Adrenal gland homogenates from rabbits stimulated for 28 days with adrenocorticotropin have been shown to synthesize cortisol. The total amount of cortisol formed was the same whether progesterone-4-(14)C or Delta(5)-pregnenolone-4-(14)C was used as exogenous substrate. However, when Delta(5)-pregnenolone substrate was used the specific activity of the isolated cortisol was four times as great as when progesterone was used.


Potassium, corticosterone, and adrenocorticotropic hormone release in vitro. Incubation of rat adenohypophyses in a high concentration of potassium increases adrenocorticotropic hormone release. This increased release is suppressed by the addition of corticosterone to the incubating medium. Our findings are consistent with a process of "stimulus-secretion coupling" proposed for other glands and suggest that corticosterone may operate directly on the adenohypophysial cell membrane to inhibit releasing mechanisms.


Encephalic cycles during sleep and wakefulness in humans: a 24-hour pattern. Twenty-four-hour polygraphic tracings from normal humans indicate that a pattern of alternating periods of the presence and absence of rapid eye movement, shown to exist for normal sleep, exist over all 24 hours of the daily period. This finding suggests that the so-called sleep-dream cycle of human sleep is not specific to sleep, but is a general activity pattern of the brain.


Calcified tissues.

Calcified tissues.


Information processing in the nervous system.


Preliminary characterization of some ectopic hormones.


The hormonal regulation of glycogen metabolism in muscle.


Effect of corticotrophin therapy on pituitary-adrenal function.


Effect of hormones on the growth of virus-transformed hamster prostatic tissue.


Rejection of skin isografts from donors with leukemia.


Purification and properties of 5-phosphoribosyl pyrophosphate amidotransferase from adenocarcinoma 755 cells.


Energy-linked reactions in photosynthetic bacteria. 3. Further studies on energy-linked nicotinamide-adenine dinucleotide reduction by Rhodospirillum rubrum chromatophores.


Promitochondria of anaerobicallly grown yeast. II. Lipid composition.


Rate of isotope exchange in enzyme-catalyzed reactions.


Electron paramagnetic resonance studies of nitric oxide hemoglobin derivatives. I. Human hemoglobin subunits.


Synthesis of acetylcholine in the electric organ of Torpedo.


A comparative study on the phosphoglyceric acid cycle in mammalian erythrocytes.


Molt cycle regulation of nucleotide pyrophosphatase in the hepatopancreas of the blue crab, Callinectes sapidus Rathbun.


Cytochrome oxidase-succinic dehydrogenase activities and the melanin pigment cycle in poikilothermic vertebrates.


The effect of starvation, feeding, glucose and ACTH on the liver glycogen levels of Tilapia mossambica.


Effect of propranolol and phentolamine on canine and bovine responses to TSH.


Effects of adrenocorticotropic hormone on rat adrenal corticosteroidogenesis in vivo.


In vivo and in vitro studies of steroid production by a luteoma and an adrenal cortical carcinoma in mice.


Failure of theophylline to potentiate stimulated thyroidal glucose oxidation and phospholipogenesis.


Effects of adrenal cortical hormones and thyroxine on phosphorylase activity in muscle.


Effects of theophylline, prostaglandin E-1 and adrenergic blocking agents on TSH stimulation of thyroid intermediary metabolism.


Adrenal cells in tissue culture. II. Steroidogenic responses to nucleosides and nucleotides.


Effect of glucagon, adenosine-3',5'-monophosphate and theophylline on free fatty acid release by rat liver slices and on tissue levels of coenzyme A esters.


Relation of the pituitary to the sodium-retaining effects of adrenal enucleation.


Preparation of 16-alpha-hydroxypregnenolone-3-sulfate and a study of its anomalous behavior during hydrolysis.


Interruption of pregnancy in the rat by administration of ACTH.


Adenyl cyclase activity in the rabbit ovary.


Protein fluorescence and inactivation of glutamate dehydrogenases during treatment with silver.


Insomnia following parachlorophenylalanine in the rat.


Effects of angiotensin--II on coronary smooth muscle.


Isolation of human leukocyte granules using colloidal silica-polysaccharide density gradients.


The primary structure of valine-I transfer ribonucleic acid from Torulopsis utilis. I. Complete digestion with pancreatic ribonuclease and ribonuclease T1.


Enzyme distribution in subcellular fractions of intestinal mucosal cells.


ESR study of L-alanine-1-13C irradiated at low temperatures.


Biochemical and morphological characterization of microvilli isolated from intestinal mucosal cells.


Note on the aetiology of acute laryngotracheobronchitis in children. Findings in the autumn of 1965 and the spring of 1966.


Degradation of cytosin-containing bacterial and bacteriophage DNA after infection of Escherichia coli B with bacteriophage T4D wild type and with mutants defective in genes 46, 47 and 56.


Antitumor immunity in the Shope papilloma-carcinoma complex. IV. Search for a transmissible factor increasing the frequency of tumor regression.


Cytomegaloviruses as common adventitious contaminants in primary African green monkey kidney cell cultures.


Liver disease, renal function, and the renin-angiotensin system.


Adrenocortical responsiveness in patients with renal transplants receiving prednisone.


Identification of purine ribosidase in the glioblastoma.


Surgical treatment of amebiasis.

Surgical treatment of amebiasis.


Local and topical anesthetics in ophthalmology.


Renal cell carcinoma in children. Report of two cases.


Estimation of renin activity in human amniotic fluid of normal and toxemic pregnant women.


The activation of virus synthesis in polyoma-transformed cells.


Viropexis of herpes simplex virus by HeLa cells.


Studies of avian leukosis group-specific complement-fixing serum antibodies in pigeons.


Isolation of viruses using specific immunoadsorbents.


E.P.R. absorption by the vapour phase of cigarette smoke, condensed and examined at low temperatures.


Carbenicillin and Pseudomonas aeruginosa infection of burns.


Polyamide-silica gel thin-layer chromatography of red food dyes.


Use of a liquid chromatograph in lipid class separation.


Enzyme diversion applied to the kinetic estimation of glucose-6-phosphatase activity.


Relationship between adipose lipoprotein lipase activity and compounds which affect intracellular lipolysis.


Chemodectoma of an aortic body.

Chemodectoma of an aortic body.


Myocardial function in patients with coronary artery disease.


Primary cystic carcinoma of the liver.

Primary cystic carcinoma of the liver.


Transient neurologic complication of positive contrast orbitography.


Recurrent herpes simplex conjunctival ulceration.


Synovial collagenase: its presence in culture from joint disease of diverse etiology.


Enzymatic synthesis of oligoguanylic acids containing 2'-5' phosphodiester linkages.


The ability of reduced nicotinamide mononucleotide to function as a hydrogen donor in the glutamic dehydrogenase reaction.


Two distinct transfer enzymes from rabbit reticulocytes with ribosome dependent guanosine triphosphate phosphohydrolase activity.


The structure of an NAD-pyruvate complex.


The structure of a free radical produced from questiomycin A. Photochemistry of antibiotics. II.


Intranuclear crystal formation of poliovirus: electron microscopic observations.


Febrile infectious mononucleosis-like syndrome following splenectomy: an example of post-transfusion mononucleosis.


Orthocresyl phosphate neuropathy: report of an outbreak in Fiji.


Radiation effects on cold-induced growth response of interscapular brown fat.


Toward control of viral infections of man.


Glucagon-sensitive adenyl cylase in plasma membrane of hepatic parenchymal cells. The plasma membrane of hepatic parenchymal cells contains an adenyl cyclase system that is stimulated by glucagon. Adrenocorticotropin and epinephrine do not stimulate this adenyl cyclase, and very little cyclic phospho-diesterase activity is present in the membrane. These findings support the concept that glucagon exerts its regulatory action in the liver by stimulating adenyl cyclase activity in the plasma membrane.


Hallucinogen-tranquilizer interaction: its nature. Study of the competition between hallucinogens and tranquilizers at cerebral synapses and on behavior in various species of animals indicates a continuum of effects from protection to dominance of tranquilizer toxicity as the dose of tranquilizer increases. Data on cat and monkey behavior, supplementing that on the rat, show that it is possible to arrive at a tranquilizer dose that can aggravate instead of protect, in accord with the competitive inhibitory nature of the interaction of hallucinogen and tranquilizer.


Some problems in the estimation of localized activity.


A bacterial factor capable of solubilizing cholinesterase from plaice body muscle.


25-Hydroxycholecalciferol, the probable metabolically active form of vitamin D. Isolation, identification, and subcellular location.


Basic studies on the mechanism of action of vitamin D.


Absorption of vitamin D3 oleate in the rat.


Vitamin D-induced calcium-binding protein of intestinal mucosa. Relation to vitamin D dose level and lag period.


Absorption and metabolism of vitamin D3 in man.


Non-staphylococcal pseudomembranous colitis.


Amebic abscess of the liver. Experience with 15 cases in 3 and one-half years in California.


Determination of free hydrogen cyanide in river water by a solvent-extraction method.


Twitch, tetanus and posttetanic potentiation as indices of nerve-muscle block in man.


Neurologic complications following spinal anesthesia with lidocaine: a prospective review of 10,440 cases.


The effects of small incremental doses of d-tubocurarine on neuromuscular transmission in anesthetized man.


Biochemical changes in rat liver in response to treatment with drugs and other agents. II. Effects of halothane, DDT, other chlorinated hydrocarbons, thioacetamide, dimethylnitrosamine and ethionine.


Biochemical changes in rat liver in response to treatment with drugs and other agents. I. Effects of anticonvulsant, anti-inflammatory, hypocholesterolaemic and adrenergic beta-blocking agents.


Acetaldehyde--an inhibitor of the enzymatic oxidation of 5-hydroxyindoleacetaldehyde.


The influence of oligomycin on the actions of epinephrine and theophylline upon the perfused rat heart.


The hydroxyproline of elastin.

The hydroxyproline of elastin.


On a relationship between ion transport and thiamine in nervous tissue.


The mechanism of binding of flavin-adenine dinucleotide to the apoenzyme of glucose oxidase and evidence for the involvement of multiple bonds.


Determination of melting sequences in DNA  and DNA-protein complexes by difference spectra. A graphical formula is presented for determining the base ratio of melted DNA. By use of this formula, the composition of sequences which melt in different portions of the melting curves of Clostridium DNA, Escherichia coli DNA, and mouse DNA were determined. As the DNA melts, the per cent of adenine and thymine (AT) in the melted sequences decreases linearly with temperature. The average composition of sequences which melt in a given part of the melting curve is proportional to the base ratio of the DNA. The concentration and average composition of sequences were determined for three parts of the melting curves of the DNA samples, and a frequency distribution curve was constructed. The curve is symmetrical and has a maximum at about 56% AT. The distribution of GC-rich sequences on the E. coli chromosome was estimated by shearing, partially melting, and fractionating the DNA on hydroxylapatite. GC-rich sequences appear to occur every thousand base pairs, and have a maximum length of about 180 base pairs. The graphical formula was applied to the determination of the composition of sequences which melt in different parts of the melting curve of chromatin. Throughout the melting curve, the composition of the melting sequences is about 60% AT, which appears to suggest that relatively long sequences are melting simultaneously. Their melting temperature may be a function of the composition of the protein on different parts of the DNA. The problem of light scattering in DNA-protein and DNA was also investigated. A formula is presented which corrects for light scattering by relating the intensity of the scattered light to the rate of change of absorbance of DNA with wavelength.


The structural basis of the catalytic function of staphylococcal nuclease.


The relationship between Mg2+-stimulated ATPase activity and glycogen content in rat liver.


Microradiographic investigations of calcifications of the female breast.


Fine structure of glomus tumor: similarity of glomus cells to smooth muscle.


Chemodectoma of the orbit.

Chemodectoma of the orbit.


Evaluation of a high dose cyclophosphamide regimen in childhood tumors.


Concomitant neoplasia in the liver of a hen.


Action of the venom of Microbracon hebetor Say on larvae and adults of Philosamia cynthia Hübn.


Peripheral neuropathies.

Peripheral neuropathies.


Reovirus-specific, single-stranded RNA's synthesized in vitro with enzyme purified from reovirus-infected cells.


Note on the uptake of serotonin by subcellular granules of brain.


Stomach cancer associated with carcinoid tumor of rectum.


Pathological study of metastatic involvements of the stomach cancers. I.


Role of haemichromes in the formation of inclusion bodies in haemoglobin H disease.


Selective inhibition of vaccinia virus by the antibiotic rifampicin.


Proinsulin and the biosynthesis of insulin.


Androgen studies in a patient with lipoid-cell tumor of the ovary.


The neuromuscular system and the re-education of movement.


Granular cell myoblastoma of the eye. Case report.


Studies of plasma renin activity in patients with hypertension.


Sodium excretion in obese adolescents.

Sodium excretion in obese adolescents.


Some renal histochemical lesions in rats chronically poisoned with carbon tetrachloride.


Oculomotor control: a possible function of rem sleep.


Adsorption of bacterial alpha-amylase on quartz and denaturation of adsorbed enzyme by shaking.


Sporadic aniridia and Wilms' tumor.

Sporadic aniridia and Wilms' tumor.


ATP dependent conformational change in "spin labelled" sarcoplasmic reticulum.


Studies on oxidative phosphorylation. XVII. Physical and chemical properties of factor B.


The incorporation of p-hydroxybenzoate and isopentenyl pyrophosphate into polyprenylphenol precursors of ubiquinone by broken cell preparations of Rhodospirillum rubrum.


Studies on ultrastructural dislocations in mitochondria. II. On the dislocation induced by lyophilization and the mechanism of uncoupling.


Isolation of chitin and glucan from the cell wall of the yeast form of Paracoccidioides brasiliensis.


Glycerolphosphate oxidoreductases and the glycerophosphate cycle in ehrlich ascites tumor cells.


The biogenesis of mitochondria. IX. Formation of the soluble mitochondrial enzymes malate dehydrogenase and fumarase in Saccharomyces cerevisiae.


The copper catalyzed oxidation of cysteine to cystine.


Horse muscle acyl phosphatase: purification and some properties.


Structural basis of group G streptococcal antigenicity.


Studies on epsilon-prototoxin of Clostridium perfringens type D. I. Purification methods: evidence for multiple forms of epsilon-prototoxin.


Studies on gluconeogenic mitochondrial enzymes. II. The conversion of glutamate to alpha-ketoglutarate by bovine liver mitochondrial glutamate dehydrogenase and glutamate-oxaloacetate transaminase.


Studies of gluconeogenic mitochondrial enzymes. 3. The conversion of alpha-ketoglutarate to glutamate by bovine liver mitochondrial glutamate dehydrogenase and glutamate-oxaloacetate transaminase.


Studies of gluconeogenic mitochondrial enzymes. IV. The conversion of oxaloacetate to fumarate by bovine liver mitochondrial malate dehydrogenase and fumarase.


Effect of exercise on alpha-glycerophosphate dehydrogenase activity in skeletal muscle.


Energy conversion-linked changes of carotenoid absorbance in Rhodospirillum rubrum chromatophores.


The enzymic synthesis of O-alpha-D-glucopyranosyl (1-6)-2-amino-2-deoxy-D-glucopyranose and its N-acetyl derivative.


On the site of discrimination of chicks against vitamin D.


Surgical management of cervical paragangliomata.


Effect of cortisone and corticotropin on gastric secretion and peptic ulceration in the dog.


Granular cell myoblastoma of the breast.


Effect of collagenase on the behaviour of cells from young and old donors in culture.


Gas chromatographic analysis of urinary 17-ketosteroids response to gonadotrophin and ACTH in young and old persons.


Intrinsic and extrinsic factors of molecular ageing.


Further implication of murine leukemia-like virs in the disorders of NZB mice. Further evidence implicating murine leukemia-like virus in the disorders of NZB mice was afforded by a study of antigens associated with murine leukemia virus (MuLV). MuLV group antigens were prevalent in extracts of spleen, kidney, and, to a lesser extent, thymus throughout a substantial portion of the life span of NZB mice as well as in extracts of lymphomas and sarcomas indigenous to the strain. G (Gross) soluble antigen, type-specific antigen, was first detected in plasma of untreated NZB mice at 3 months of age. G soluble antigen production increased thereafter in line with age, with 50% of reactions becoming positive at 5.3 months and 100% at 7 to 9 months. From months 3 to 9, the time-response curve for positive conversion of direct antiglobulin (Coombs) tests in untreated NZB mice corresponded closely to that for G soluble antigen production. Beyond the 9th month, G soluble antigen underwent elimination from the plasma of NZB mice, with positive reactions reduced to 50% at 13.3 months and to 0% at 18 months. G natural antibody was first detected in the serum of NZB mice at about 10 months of age and increased thereafter in line with age. The curves for G antibody production and G soluble antigen elimination bore a reciprocal relation to each other with crossover at 50% response occurring at 13.3 months. Significant proteinuria, a functional manifestation of membranous glomerulonephritis, became increasingly prevalent in female NZB mice as G soluble antigen was eliminated from plasma. Cumulative mortality of female NZB mice, mainly attributable to renal glomerular disease, increased in phase with G antibody production. MuLV group antigens were identified in the glomerular lesions by the immunofluorescence method. Positive conversion of direct antiglobulin tests was significantly delayed by vaccinating baby NZB mice with formaldehyde-inactivated cell-free filtrates of older NZB mouse spleens. The plasmas of vaccinated NZB mice with negative direct antiglobulin reactions at 4 to 7 months were likewise negative when tested for G soluble antigen. The 50% response time for G antibody production in the vaccinated NZB mice occurred at 7.3 months, that is, 6 months earlier than in untreated NZB mice. The collective findings implicate murine leukemia-like virus in the etiology of autoimmune hemolytic disease and membranous glomerulonephritis, as well as malignant lymphoma, of NZB mice and suggest that virus-specified cell-surface and soluble antigen is a factor in the immunopathogenesis of the renal disease and possibly also the autoimmune hemolytic disease.


Peripheral dysostosis: investigation of metabolic and endocrine functions.


A case of Bartter's syndrome with abnormal renin response to salt load.


Congenital cytomegalovirus infection associated with low birth weight.


Rhinovirus infections in nursery school children.


Plasma renin in Bartter's syndrome: response of depression of stimuli for renin release.


A case of glycogen storage disease.

A case of glycogen storage disease.


Metabolism and serum protein binding of 3H-vitamin D4 in vitamin D-resistant rickets.


Growth in familial hypophosphatemic vitamin D-resistant rickets.


Further experience in treatment of juvenile nasopharyngeal angiofibroma.


Here today--gone today.

Here today--gone today.


An epidemiologic study of altered clinical reactivity to respiratory syncytial (RS) virus infection in children previously vaccinated with an inactivated RS virus vaccine.


Respiratory syncytial virus disease in infants despite prior administration of antigenic inactivated vaccine.


Field evaluation of a respiratory syncytial virus vaccine and a trivalent parainfluenza virus vaccine in a pediatric population.


Respiratory virus immunization. I. A field trial of two inactivated respiratory virus vaccines; an aqueous trivalent parainfluenza virus vaccine and an alum-precipitated respiratory syncytial virus vaccine.


Epidemiologic studies of latent virus infections in captive monkeys and baboons. I. Overall plans and virus isolations with special reference to SV40 and foamy viruses.


Epidemiologic studies of latent virus infections in captive monkeys and baboons. II. Serologic evidence of myxovirus infections with special reference to SV5.


Experimental infection of monkeys with Langat virus. 3. Infection produced by virus feeding.


Disseminated herpes simplex in a newborn. A consequence of infection in the mother.


Hypothalamic pituitary stimulating and inhibiting hormones.


Drugs and sleep.

Drugs and sleep.


An electron cytochemical demonstration and biochemical analysis of adenosine triphosphatase activity in cancer cell plasma membrane.


Neutralization of the anticomplementary activity of an amorphous silica by some proteins.


Formation of thymyl radicals in gamma-irradiated frozen aqueous systems: as E.S.R. study.


Liver enzyme histochemistry: a comparative study of sheep, calf and rat.


Control of renin secretion in the anesthetized dog. 1. Relationship between renin secretion and plasma sodium concentration in the peritoneal dialyzed dog.


The role of the adrenals in changes of liver triglycerides content after partial hepatectomy in rats of different ages.


Anti-I agglutinins in non-human sera.

Anti-I agglutinins in non-human sera.


Hypovolemic shock: microcirculatory changes during and after specific therapy.


Islet cell tumors and hypoglycemia.

Islet cell tumors and hypoglycemia.


Activation and inhibition of purified phosphotransacetylase of Escherichia coli B by pyruvate and by NADH2 and certain nucleotides.


Clostridium perfringens polynucleotide phosphorylase: interconversion of heavy and light molecular weight species.


Inhibition of beta-D-galactosidases by D-galactal.


Tryptophan-mediated substrate inhibition of anthranilate-5-phosphoribosylpyrophosphate phosphoribosyltransferase.


Thymidine salvage pathway in normal and leukemic leukocytes with effects of ATP on enzyme control.


Hormones in human pituitary sinusoid blood: concentration of LH, GH and TSH.


Cytomegalic adrenocortical hypoplasia and increased plasma 20-alpha-hydrozypregn-4-en-3-one in a man exhibiting the features of selective mineralocorticoid deficiency.


Comparison of the effect of ACTH and an inhibitor of 3-beta-hydroxysteroid dehydrogenase on the synthesis of adrenal microsomal proteins.


Hepatic lobectomy in childhood: effectws of x-ray and chemotherapy.


Determination of corticosteroid-binding proteins by an adsorption method.


Compounds which stabilize and organize the tetrameric isozymes of lactate dehydrogenase.


Histochemistry of 3 beta-hydroxysteroid dehydrogenase in human adrenal cortex in various pathological conditions.


Properties of phosphorylase kinase and its control in skeletal muscle.


The role of cyclic AMP in the control of liver metabolism.


Hormonal and non-hormonal control of glycogen synthesis-control of transferase phosphatase and transferase I kinase.


Nucleic acids of tumor viruses.

Nucleic acids of tumor viruses.


The effects of ethanol on the metabolic activities of the liver.


Spectrofluorometry of dihydroxyphenylacetic acid in urine.


The occurrence of bone and cartilage in mammary tumors.


The chemical senses.

The chemical senses.


Adrenal response to ACTH in patients with Prader-Willi syndrome, simple obesity, and constitutional dwarfism.


Laboratory diagnosis of Japanese encephalitis. Comparison of the fluorescent antibody technique with virus isolation and serologic tests.


Inhibition of L-alpha-glycerophosphate dehydrogenase by alkylammonium chlorides.


Different responses of white and brown adipose tissue to drugs affecting lipolysis.


Mechanism of inhibition of histidine decarboxylase (Clostridium welchii) by 4-bromo-3-hydroxybenzyloxamine and amino-oxyacetic acid.


Increased monoamine oxidase activity in isoproterenol-stimulated submaxillary glands.


Chemodectoma.

Chemodectoma.


Serum inhibitors of myxoviruses.

Serum inhibitors of myxoviruses.


The synthesis of hyaluronic acid in vitreous. II. The presence of soluble transferase and nucleotide sugar in the accellular vitreous gel.


Diffuse external otitis--treatment of 175 ears with a new preparation (Synalar Otic).


Polyamide layer chromatography of organophosphorus pesticides.


Studies of metyrapone responses in patients with pituitary disease.


A comparison of the in-vitro actions of thyroid-stimulating hormone and cyclic 3',5'-adenosine monophosphate on the mouse thyroid gland.


A modified ovarian cholesterol depletion assay for luteinizing hormone.


Psychology of the scientist. 28. Speculations on nonverbal creativity.


The effect of cobalt on the ultrastructure of mitochondria.


Radiological characteristics of brain tumors infiltrating corpus callosum.


Sleep disturbances in the acute alcoholic psychoses.


Effects of chemotherapeutic agents on two lines of human breast carcinomas in tissue culture.


Gluconeogenesis in biotin deficiency: in vivo synthesis of pyruvate holocarboxylase in biotin deficient rat liver.


The physicochemical basis of the functioning of biological membranes: the conformation of valinomycin and its K+ complex in solution.


Purification and properties of 2, 3-diphosphoglyceric acid phosphatase from human erythrocytes.


Steroid dehydrogenases and steroids in gonads of the seaperch Cymatogaster aggregata Gibbons.


Blood glucose in male toads (Bufo bufo): annual variation and hormonal regulation.


Some evidence for the presence of a corticotrophin releasing factor (CRF) in the duck (Anas platyrhynchos).


Prolonged ganglionic facilitation and the positive afterpotential.


Comparative investigation (skin tests) with natural and synthetic corticotrophin in allergic patients.


Effects of adrenocorticotropic hormone on avoidance behaviour of intact and adrenalectomized rats.


Possible reactivating and sensitizing action of neuromyally acting agents.


Influence of microorganisms on oxygen consumption, carbon dioxide production and colonic temperature of rats.


Modification of epinephrine-induced inhibition of transmission by tropolone and desipramine (desmethylimipramine) in the perfused superior cervical ganglion.


Microbial contamination of antibiotic ophthalmic ointments.


Aflatoxicosis and trout hepatoma.

Aflatoxicosis and trout hepatoma.


Changes in ultrastructure and electroretinogram of bulfrog retina during development.


Calcification in mucinous cell carcinoma of the stomach.


A comparative study of the antitumor and antiviral activity of 1-beta-D-arabinofuranosyl-5-fluorocytosine (FCA) and 1-beta-D-arabinofuranosylcytosine (CA).


Functional reactivation and cytological restoration of pituitary grafts by continuous local intravascular infusion of median eminence extracts.


Mouse bone collagenase: isolation, partial purification, and mechanism of action.


Effects of growth hormone administration on hepatic excretion of bromsulfalein (BSP).


Effects of pargyline and amphetamine upon acute stress responses in rats.


Temporal protection of chick embryo against endotoxin by isoproterenol, polymyxin B sulfate, and hydrocortisone.


Interferon action in heterokaryons.

Interferon action in heterokaryons.


Hormonal regulation of myocardial adenosine 3',5'-monophosphate.


The role of the alpha-glycerophosphate cycle in the control of carbohydrate oxidation in heart and in the mechanism of action of thyroid hormone.


UDPglucose: alpha-I,4-glucan alpha-4-glucosyltransferase in heart regulation of the activity of the transferase in vivo and in vitro in rat. A dissociation in the action of insulin on transport and on transferase conversion.


Subcellular distribution of 3-hydroxy-3-methylglutaryl-CoA synthase, acetoacetyl-CoA thiolase and NAD kinase in Saccharomyces cerevisiae.


The influence of insulin and epinephrine on adenosine 3',5'-phosphate and glycogen transferase in muscle.


The Reynold L. Haas Memorial Lecture: gynecologic curiosities and concepts.


Formation of a cobamide containing divalent cobalt by the ribonucleotide reductase of Lactobacillus leichmanii.


Fructose-1,6-diphosphatase and phosphopyruvate carboxykinase in bovine lactating mammary gland.


Nucleotide diphosphate sugar pyrophosphatase activity in the skin of alloxan-diabetic rats.


Catecholamine synthesis in rabbits with neurogenic hypertension.


Mixed tumors of salivary glands: a report from the Tumor Registry of the University of Michigan Medical Center.


Gel filtration behavior of calcium and magnesium salts in normal and stone-forming urines.


A general colorimetric procedure for the estimation of enzymes which are linked to the NADH-NAD+ system.


Variant alkaline phosphatase in human hepatocellular cancers.


Parotid fluid cortisol and cortisone.

Parotid fluid cortisol and cortisone. Parotid fluid corticosteroids, substantially comprised of cortisol and cortisone, were previously demonstrated to rise to far greater levels 4 hr after administration of ACTH than they did in the third trimester of pregnancy, although the plasma total corticosteroid concentrations were similar in these two states. It was therefore suggested that only nonproteinbound corticosteroid gains access to parotid fluid. In the present study parotid fluid cortisol and cortisone and plasma dialyzable cortisol concentrations have been measured in normal men before and 2 hr after 40 U ACTH, and, in another group, before and after 10 days of diethystilbestrol (5 mg daily). Total plasma cortisol rose from a mean of 6.3 to 17.9 mug/100 ml after ACTH and from 14.6 to 39.4 mg/100 ml after the estrogen. However parotid fluid cortisol plus cortisone rose from 0.8 to 2.6 mug/100 ml after ACTH and to only 2.2 after estrogen. This rise resembled that of the plasma dialyzable cortisol (control 0.4, ACTH 1.8, estrogen 1.2 mug/100 ml) rather than the increase in total plasma cortisol which was over twice as high after estrogen as after ACTH. Thus parotid fluid corticosteroids seem to be a good measure of nonprotein-bound corticosteroid, the cortisol available to the cell. The total amount of cortisol plus cortisone excreted is approximately constant, independent of parotid fluid flow rate. Cortisone exceeds cortisol in parotid fluid in the basal state, but after ACTH the situation is reversed.


Plasma progesterone and 17-hydroxyprogesterone in normal men and children with congenital adrenal hyperplasia. Plasma 17-hydroxyprogesterone (17-OHP) concentrations in normal men averaged 0.094 mug/100 ml. Studies using suppressive doses of androgens and glucocorticoids showed that 90% of the 17-OHP originated from the Leydig cell. The 17-OHP production rate was 1.8 mg/24 hr. Plasma 17-OHP has a marked circadian variation, the 8 p.m. values being only 40% of the 8 a.m. values. Plasma luteinizing hormone measured in the same samples did not vary. The adrenal cortex has the capacity to synthesize and secrete 17-OHP and progesterone since adrenocorticotrophic hormone (ACTH) caused a fourfold increase in these plasma steroids. In children with congenital adrenal hyperplasia, plasma 17-OHP levels were 50-200 times those of normal men and plasma progesterone was increased 6- to 10-fold over normal men.


A gel diffusion precipitin test for bovine papilloma virus.


Mixed hemadsorption technique for study of avian leukosis-sarcoma viruses: standardization of the technique.


Mixed hemadsorption technique for study of avian leukosis-sarcoma viruses: studies with avian leukosis viruses.


Search for natural nidality of influenza and parainfluenza viruses in Illinois.


Immunodepression by viruses.

Immunodepression by viruses.


Immunogenicity of namogram to milligram quantities of inactivated foot-and-mouth disease virus. I. Relative virus-neutralizing potency of guinea pig sera. Quantitative antigen dose-neutralizing antibody response curves were established in guinea pigs for purified foot-and-mouth disease virus (FMDV), type A, strain 119, inactivated for 48 hr with N-acetylethyleneimine (AEI). Inactivation of FMDV by 0.05% AEI at 25 C occurred without virus degradation and followed first-order kinetics over a 10(8)-fold decrease in plaque-forming units (PFU) extrapolating to 10(-5) PFU/ml at 48 hr. The AEI-treated virus was administered in doses ranging from 10 ng to 2.62 mg, alone or emulsified in oil adjuvant. Sigmoidal dose-response curves were obtained with 160 ng as the minimum effective dose. The maximum effective dose was 163 mug and 2.62 mg or more at 6 and 28 through 84 days postinoculation, respectively. Oil adjuvant had little effect at 6 days postinoculation, but its use markedly increased the amount of neutralizing antibody obtained at the later testing periods.


Rapid identification of viruses by indirect immunofluorescence: standardization and use of antiserum pool to nine respiratory viruses. The indirect fluorescent antibody test employing treated and standardized antisera and conjugated antiglobulin has been used successfully, in conjunction with a technique for growing and staining virus cell systems in situ on microscope slides, in the identification of nine respiratory viruses. By using pooled antisera in a single test, the presence or absence of these viruses was determined in 18 to 45 hr after inoculation of slide microtissue culture.


Delay of poliovirus plaque formation with use of fresh bovine serum in the growth medium of host cells. The plaque formation of poliovirus in HeLa cell monolayers was decreased upto 95% when the host cells were subcultured several times in a growth medium containing fresh bovine serum prior to their inoculation with virus. Extended incubation of infected cell monolayers indicated that the inhibition was in the form of a delay in plaque formation rather than a permanent inhibition of it. The inhibitory factor, which was found in most bovine sera, was very unstable and disappeared after storage of the serum at -20 C for a few weeks or at 36 C within a few days. In HeLa-cell monolayers, the fresh serum brought about a decrease in the plaque formation of all three poliovirus types as well as that of poliovirus ribonucleic acid. The plaque formation of poliovirus in monkey heart and HEp-2-cell monolayers was decreased irregularly by the use of fresh serum in the growth medium of these cells. Speculations were made as to the possible mode of action of the bovine serum inhibitor.


Modified Sabouraud medium containing neomycin and polymyxin. A commercially available antibiotic mixture effectively decreased overgrowth of Sabouraud medium by gram-negative flora frequently found in the sputum of hospitalized patients.


Foot-and-mouth disease virus: stability of neutralizing antibody after freeze-drying and air-drying. It is possible to stabilize foot-and-mouth disease antiserum by either freeze-drying in bottles or air-drying on paper for periods of 7 months.


Effects of actinomycin D on the oligonucleotide composition of nucleolar 45-S RNA of Novikoff hepatoma ascites cells.


Herpes zoster and multiple sclerosis.

Herpes zoster and multiple sclerosis. No significant difference was found between 50 consecutive patients with multiple sclerosis and matched controls in respect of previous infection with rubella or measles and chicken-pox, or of previous vaccination and immunizing injections. Significantly more patients had a past history of herpes zoster compared with the controls.


Influence of dichlorophenylamino-imidazoline on the pressor response in the rat.


Studies on the biosynthesis of the lipid and protein components of a membraneous cytochrome oxidase preparation of rat liver mitochondria in vivo.


Separation of cyclic-3',5'-adenosine monophosphate from other adenine nucleotides.


Hormonal and nutritional requirements for limb regeneration and survival of adult newts.


The role of a renal thirst factor in drinking induced by extracellular stimuli. 1. Rats in normal fluid balance drank water 1-2 hr after complete ligation of the inferior vena cava either above or below the renal veins. At the same time there was a fall in urine flow and excretion of electrolyte, especially after caval ligation above the renal veins, so that the animals ended the initial 6 hr period in positive fluid balance.2. Caval ligation was relatively ineffective as a stimulus to drinking after bilateral nephrectomy, but was effective in rats made anuric by ureteric ligation.3. Rats subjected to caval ligation and offered a choice between water and 1.8% saline (w/v) drank water, despite the increasing hypotonicity of the body fluids thereby resulting.4. During the secondary polydipsia, which generally occurred on about the third day after caval ligation as renal function was recovering, there was an increased preference for 1.8% saline.5. Constriction of the aorta above the renal arteries, or constriction of both renal arteries, also caused drinking, oliguria and the development of positive fluid balance.6. Constriction of the aorta below the renal arteries, or after nephrectomy, was ineffective as a stimulus to drinking.7. Saline extracts of renal cortex caused rats in normal water balance to drink. Activity was destroyed by boiling the extract for 10 min. Renal medullary and hepatic extracts were without effect on drinking.8. It proved impossible to separate dipsogenic and pressor activities of renal extracts during the different stages of fractionation which lead to the production of renin; disappearance of one activity was invariably accompanied by disappearance of the other.9. Dipsogenic and pressor actions were greater in nephrectomized rats than in normal rats.10. Both extractable dipsogenic factor and extractable pressor activity were reduced by treating the rat with DOCA and saline for several weeks beforehand.11. The renal dipsogen therefore has similar properties to renin. It may prove to be identical with renin, particularly in view of the fact that angiotensin also stimulates drinking.12. Adrenalectomy did not affect drinking induced by renin or by caval ligation.13. It is concluded that the renin angiotensin system may play a role in the genesis of the thirst which follows certain extracellular stimuli.


Observations on an effect of ACTH, dexamethasone, and dimethyl sulfoxide on the "out of strain" transplantation and lethality of strain 2 guinea pig leukemia L2C-NB to strain 13 and Hartley animals.


Geriatrics.

Geriatrics.


Bacteremia and thrombus accumulation on prosthetic heart valves in the dog.


Courage to change.

Courage to change.


Morphological and biochemical studies of virus (SV40) transformed prostatic tissue.


Modifying behavior: the case of Alice.

Modifying behavior: the case of Alice.


The critical incident form as a tool.

The critical incident form as a tool.


Acute cardic care; the role of the registered nurse. A joint statement by the Minnesota State Medical Association, the Minnesota Heart Association and the Minnesota Nurses Association.


The 1969 look in drug abuse control.

The 1969 look in drug abuse control.


Mental health, psychotherapy, and the new psychotherapist.


Woodbridge State School.

Woodbridge State School.


Perception of nursing role in a developing mental health center.


Viral flora of primate tissues--discussion.


Viral flora of tissue sources--simian and human.


Training psychiatric nurses in developing countries.


Viral oncogenesis and results of inoculation of normal and transformed cells into nonhuman primates.


Characteristics of cell culture systems. Viral flora of canine tissues.


Attitudes and behavior in response to nursing staff in mufti.


Viral flora of chick and duck tissue sources.


Latent viral infections in donor tissues and in recipients of vaccines.


Variable susceptibility of human cell strains to SV40 transformation.


Documentation and classification of oncogenic viruses.


Detection of latent viruses in kidney tissues.


Cooperative and antagonistic interactions among RNA tumor viruses.


Viral oncogenicity and viral vaccines--general comments.


Monitoring vaccines for human oncogenicity.


Monitoring vaccines for human oncogenicity--discussion.


Identification of established cell lines with reference to karyology.


Veterinary vaccines.

Veterinary vaccines.


Experience with an established cell line for veterinary vaccine production.


Experience with vaccines--general comments.


Rheumatoid arthritis. 2. Systemic manifestations.


Large-scale cultivation of animal cells in roller bottles for production of decigram amounts of pure foot-and-mouth disease virus.


Sleep, dreams and computers.

Sleep, dreams and computers.


Immunological aspects of carcinogenesis.


The interaction of chemical carcinogens and cells in vitro.


Effect of flavonoids and related substances. II. Antiviral effect of quercetin, dihydroquercetin and dihydrofisetin.


Quantum-mechanical investigations of the electronic structure of nucleic acids and their constituents.


Observations on the clinical features of carcinoma of the pancreas.


Lipiodol absorption and urinary lodide excretion as a screening test for steatorrhea.


The preparation and properties of deflavo xanthine oxidase.


Conversion of light to chemical free energy. I. Porphyrin-sensitized photoreduction of ferredoxin by glutathione.


Sugar phosphate phosphohydrolase. II. Effects of glucose on its activity and biosynthesis in Neisseria meningitidis.


Mechanism of action of amino acid transfer ribonucleic acid ligases.


Destruction of insulin effector system of adipose tissue cells by proteolytic enzymes.


The separation of the phosphodiesterase and deoxyribonuclease II activities of bovine spleen.


Regulatory mechanisms involving nicotinamide adenine nucleotides as allosteric effectors. I. Control characteristics of malate dehydrogenase.


The nature of tetraphenylboron-dissociated brain and brain tumor cells.


Optical rotatory dispersion of mitochondrial reduced diphosphopyridine nucleotide dehydrogenase from cardiac tissue.


Glycogenolytic effect of dibutyryl cyclic adenosine 3',5'-monophosphate in perfused rat liver.


Stress-induced alterations in arylesterase activity in the rat.


The effect of ethanol and cyanide on NAD-NADH2 ratios in rat liver.


The biochemical mechanism of action of the antinematodal drug tetramisole.


Hedamycin and rubiflavin complexes with deoxyribonucleic acid and other polynucleotides.


Studies on poisoning by Macrozamia communis. I. Biochemical disturbances in the liver.


Effects of chlorpromazine on behaviour of some enzymes of rat liver in different experimental conditions.


Studies on the mechanism of inhibition of glutamine synthetase by methionine sulfoximine.


Interaction of the 5-methylphenazinium cation radical with deoxyribonucleic acid.


Inhibition of phospholipase C by phosphonate analogs of glycerophosphatides.


Rat kidney glycerylphosphorylcholine diesterase.


Studies on the kinetics of formation and dissociation of the actomyosin complex.


The structures of enzymically produced diphosphoinositide and triphosphoinositide.


The turnover of myelin in the adult rat.


The detection of lipids on thin-layer chromatograms with the periodate-Schiff reagents.


Factors in the diagnosis of breast disease.


Studies on the reaction of cytochrome c with corticosteroids.


Adenoid cystic carcinoma of the breast.

Adenoid cystic carcinoma of the breast.


Interactions of parathyroid hormone, thyroxine and calcitonin.


Simulation of phosphate excretion by the renal arterial infusion of 3'5'-AMP (cyclic AMP)-a possible mechanism of action of parathyroid hormone.


Changes in DNA-bound amino acids in experimental tumor transplants.


The sex-dependent difference in the development of liver tumors in mice administered dimethylnitrosamine.


Acid hydrolase activity during the induction and transplantation of hepatomas in the rat.


Inhibition of the growth of mouse polyoma tumors by lymph node fragments from specifically immunized rats.


Stimulation of aldosterone secretion by sodium depletion in nephrectomized rats.


In vivo effect of sex hormones on the in vitro synthesis of prolactin and growth hormone in normal and pituitary tumor-bearing rats.


Sensitivity of the adrenal corticosterone response to ACTH as a function of time after hypophysectomy.


The effect of heat-treatment and UV-irradiation on x-ray induced electron spin resonance centers in valine.


Effects of dopamine and ACTH on steroid sensitive single neurones in the basal hypothalamus.


The effect of haloperidol on the sleep cycle of the cat.


Liver regeneration after partial hepatectomy in rats exposed before the operation to the stress stimulus.


Adrenal and clinical responses to corticotrophin and prednisone in inflammatory disease of the colon.


Response of the adrenal gland to the activation of the subcommissural organ in the pigeon.


Dehydrogenase and nucleoproteid histochemistry of the juxtaglomerular complex.


Penetration of circulating fluorescent proteins into walls of arterioles and venules in rats with intermittent acute angiotensin-hypertension.


Cancer metastasis to the cirrhotic liver. A study of experimental and autopsy material.


Improved methods for gonococcal sampling and examination on a large scale.


Respiratory syncytial virus complement fixing antibodies. Evidence of the 7S nature of antibodies determined with dextran added to the test system.


Distribution of protein-bound N-acetylneuraminic acid in subcellular particulate fractions prepared from rat whole brain.


Ketogenesis in vitro.

Ketogenesis in vitro.


Sources of nitrogen as rate-limiting factors for purine biosynthesis de novo in Ehrlich ascites tumor cells.


Value of tomography in the diagnosis of the glomus jugulare tumors.


Inhibition by pyrophosphate and polyphosphate of aortic calcification induced by vitamin D3 in rats.


Incorporation of phospholipid precursors into isolated rat liver mitochondria.


Breakdown of double-stranded RNA by bull semen ribonuclease.


The susceptibility of Clostridium perfringens type A to ampicillin and penicillin G in vitro.


Studies of the energy-transfer system of submitochondrial particles. Kinetic studies of the effect of oligomycin on the respiratory chain of EDTA particles.


A convenient spectrophotometric assay for phosphodiesterases, using dinucleoside-monophosphates as substrates.


Stimulation of fructose 1,6-diphosphatase by sulfhydryl reagents and interaction between the different enzymic sites in the molecule.


Cytochrome oxidase apoprotein in "petite" mutant yeast mitochondria. Reconstitution of cytochrome oxidase by combining apoprotein with cytohemin.


Experimental studies on the effect of antibiotics and anticancer compounds on the phagocytic activity of the reticuloendothelial system (R.E.S.).


The nucleotide sequence of N-formyl-methionyl-transfer RNA. Products of complete digestion with ribonuclease T-1 and pancreatic ribonuclease and derivation of their sequences.


Study of a blood-free medium for transport and growth of Bordetella pertussis.


Post synaptic stimulation of the electrogenic sodium pump.


Bronchiolar (alveolar cell) carcinoma of lung.


Recent advances in antibiotics.

Recent advances in antibiotics.


The carcinogenic action and metabolism of urethan and N-hydroxyurethan.


Runting syndromes, autoimmunity, and neoplasia.


Cyclic AMP: role of the medicinal chemist.


Hormonal effect on nucleolar RNA polymerase in normal liver and hepatoma cells.


Glycolytic regulation in rat liver and hepatomas.


Cyclic 3',5'-nucleotide phosphodiesterase. A continous titrimetric assay.


Analysis of nucleic acid derivatives at the subnanomole level. 3. A tritium labeling procedure for quantitative analysis of ribose derivatives.


Enzymic assay method for inorganic pyrophosphate.


Synthesis of nicontinamide-adenine dinucleotide by NAD pyrophosphorylase on a column of hydroxylapatite.


Enzymic analysis of cyclic 3', 5'-AMP in mammalian tissues and urine.


Biochemical changes in rat liver in response to treatment with drugs and other agents. 3. Effects of centrally acting drugs.


On the heterogeneity of skeltal muscle fibers: the intermediate fibers.


Effect of theophylline on acid secretion of stimulated human gastric mucosa.


Studies on the adaptation of Afircan horse sickness virus to adult hamsters.


Phospholipids of Nocardia coeliaca.

Phospholipids of Nocardia coeliaca. The lipids of Nocardia coeliaca were separated into at least 10 components by the use of thin-layer chromatography. Phosphatidylcholine was the most abundant phospholipid in this organism, accounting for 25 to 40% of the total phospholipids. The major fatty acid components of the phosphatidylcholine were 14-methyl-pentadecanoic acid (41%), the other C(15) and C(17) iso- and anteiso-fatty acids (29%), and palmitic acid (13.5%). The next most abundant phospholipid was phosphatidylethanolamine (25 to 30%), followed by phosphatidylinositol (11 to 14%) and cardiolipin (7 to 15%). Phosphatidylethanolamine and phosphatidylinositol were very similar to the phosphatidylcholine in fatty acid composition, whereas cardiolipin was characterized by a higher content of palmitic acid (30%). In all of the phospholipids examined, only trace amounts of monounsaturated fatty acids were present. When washed cells of N. coeliaca were incubated with methionine-methyl-(14)C for 1 to 3 hr, the radioactivity was mainly incorporated into the choline moiety of the phosphatidylcholine. In contrast, acetate-1-(14)C or glycerol-1-(14)C was incorporated much more slowly into the phosphatidylcholine than into the other phospholipids and neutral lipids. No phosphatidylcholine was detected in 10 other species of Nocardia examined.


Restoration by ubiquinone of Azotobacter vinelandii reduced nicotinamide adenine dinucleotide oxidase activity. Extraction with pentane virtually abolished reduced nicotinamide adenine dinucleotide oxidase activity in small particles from Azotobacter vinelandii, but activity was largely restored by added ubiquinone.


Radical formation in x-irradiated glycine HC1.


The neuroanatomy of Hymenolepis diminuta and H. nana.


The influence of 5HT on the heart phosphorylase activity in the snail, Lymnaea stagnalis L.


Chromatin phosphatides and phosphoprotein.


The use of estrone-protein conjugates in the study of the in vivo fate of antigen.


Effect of muscle acyl phosphatase on the amino acid incorporation in cell-free systems.


Renal hypertension in rabbits immunized with angiotensin.


Understanding physical change.

Understanding physical change.


Diseases of sweat and sebaceous glands, hair and nails.


Accumulation of 3H-noradrenaline in cardiac and smooth muscle of rats with experimental hypertension.


Cancer of the gastrointestinal tract in young people.


Studies of control of tissue protein levels: differential responses of tumor and liver to fasting.


Synaptic responsiveness in the lateral geniculate nucleus of the alert cat.


The effect of retinal illumination and retinal blockade on synaptic transmission in the lateral geniculate nucleus of the cat.


Cytomegalovirus infection during pregnancy. A prospective study and report of six cases.


D-Fructose-1,6-diphosphatase of navy bean (Phaseolus vulgaris L.) leaves.


ESR studies of copper(II) complex ions.

ESR studies of copper(II) complex ions.


The interaction of organic and inorganic phosphates with hemoglobin.


Studies on ribonuclease produced by Rhizopus sp. I. Crystallization and some properties of the ribonuclease.


Allergic encephalomyelitis: cleavage of the C-tryptophyl bond in the encephalitogenic basic protein from bovne myelin.


Control of ATP-dependent CO2 fixation in extracts of Hydrogenomonas facilis: NADH regulation of phosphoribulokinase.


Inhibitors of photophosphorylation and photoreduction by chromatophores from Rhodospirillum rubrum.


Inhibition of energy transfer reactions in mitochondria by hydroxylamine.


Incorporation of tritiated thymidine into the cytoplasm of PAS positive reticular cells in x-irradiated mouse thymus.


Eye signs of hypophosphatasia.

Eye signs of hypophosphatasia.


Multiple congenital abnormalities associated with cryptophthalmia.


A new enzyme for the interconversion of pyruvate and phosphopyruvate and its role in the C4 dicarboxylic acid pathway of photosynthesis. 1. An enzyme was isolated from leaves of tropical grasses that catalyses the reversible conversion of pyruvate, ATP and orthophosphate into phosphopyruvate, AMP and pyrophosphate. A requirement for Mg(2+) could not be replaced by Mn(2+) or Ca(2+). 2. By replacing orthophosphate with [(32)P]orthophosphate or with arsenate, evidence was provided that the orthophosphate consumed appears in pyrophosphate. 3. Without Mg(2+) or 2-mercaptoethanol the enzyme was rapidly and irreversibly inactivated. EDTA only partially replaced the requirement for the thiol compound. The enzyme was considerably more unstable at 0 degrees or when frozen than at 22 degrees . Even with the best conditions devised the enzyme lost about 25% of its activity every 3hr. 4. The activities of the enzyme in leaves of the tropical grasses sugar cane (Saccharum hybrid var. Pindar), maize (Zea mays) and sorghum (Sorghum vulgare) were comparable with their maximum photosynthesis rates. The enzyme was not detectable in leaf extracts from several other plants. 5. Its role in photosynthesis is discussed.


Sources of alcohol dehydrogenase (NAD+-linked) in the cockroach, Leucophaea maderae.


Regulation of NAD-specific alcohol dehydrogenases in Neurospora crassa.


The direct irradiation of cerebral gliomata.


A technique of two phase carotid angiopolygraphy.


Linitis plastica of the colon.

Linitis plastica of the colon.


Effect of ACTH and metyrapone on estriol, 17-hydroxycorticosteroid, 17-ketosteroid, pregnanediol and pregnanetriol excretion late in pregnancy.


Thyroid iodine content and turnover in euthyroid subjects: validity of estimation of thyroid iodine accumulation from short-term clearance studies.


Evidence that chlorpropamide and vasopressin share a common site of action.


Temporal aspects of anticholinergic-and anticholinesterase-induced amnesia for an appetitive habit.


Retrograde amnesia produced by hypothermia in rats.


Idiopathic hypoglycemia: a study of twenty-six children.


Virus-like particles in sera of patients with infectious and serum hepatitis.


Hepatitis virus at last?

Hepatitis virus at last?


Adenovirus infection in intussusception in children in Taiwan.


Prevalence of antibodies to EB virus and other herpesviruses.


Acute gout--definitely a medical emergency.


Mistaken diagnosis of benign parotid tumor with facial paralysis.


Paget's disease in the female breast.

Paget's disease in the female breast.


Infectious replicative intermediate of poliovirus: purification and characterization.


Analysis of poliovirus-specific RNA in infected HeLa cells by polyacrylamide gel electrophoresis.


Some characteristics of an isolated group antigen common to most strains of murine leukemia virus.


The interaction of tritium-labelled mengo virus RNA and L cells: the effects of DMSO and DEAE-dextran.


Absence of repressor in SV40-transformed cells.


Correlation between the synthesis of DNA and histones in polyoma virus-infected mouse embryo cells.


Utilization of substrate analogs by mengovirus induced RNA polymerase.


The ionization constant of nicotinamide-adenine dinucleotide.


Postirradiation malignant neoplasms of the uterine fundus.


New fluorometric method for the determination of cortisol in serum.


Histochemical observations on the developing foot of normal, oligosyndactylous (Os-plus) and syndactylous (sm-sm) mouse embryos.


Lack of cell proliferation in the foci of variola virus-infected FL cells.


Immune hemadsorption by cells infected with poxviruses.


L.C.A.O.-M.O. calculations on isomeric methyleneindenes.


Sleep patterns during afternoon naps in the young and elderly.


Hyperemia of the salivary gland in cytomegalic inculusion disease.


An electron microscopic study of human salivary gland tumors. Pleomorphic adenoma and adenoid cystic carcinoma.


Effects of some anticholinergic drugs on antidromic activity in the rat phrenic nerve-diaphragm preparation.


Response of the nictitating membrane to prostaglandin E1 and angiotensin.


Catalase of tumor-bearing animals. I. Combined immunochemical and enzymic determination of catalase in liver cell fractions.


Catalase of tumor-bearing animals. II. Chromatographic analysis of liver catalase.


Antitumor effect of actinomycin-D on transplantable Wilms tumor in rats.


Distribution of N-methyl [14C]-bis (3-mesyloxypropyl)-amine hydrochloride and its metabolites in normal and tumor-bearing rats.


Establishment of a milk agent-free substrain C3HeB-Os and effect of foster nursing on occurrence of mammary tumors.


Effectiveness of cytosine arabinoside therapy on leukemia (L-1210) of the central nervous system in mice arising during methotrexate treatment.


The antiviral activity of 9-beta-D-arabinofuranosyladenine (ARA-A).


Induction of liver tumor in mice injected at birth with liver tumor extract.


Thymidine kinases from normal and tumor tissues.


The lens capsule. Studies on the carbohydrate units.


The enzymes of the galactose operon in Escherichia coli. II. The subunits of uridine diphosphogalactose 4-epimerase.


Stimulation of tryptophanase synthesis in Escherichia coli by cyclic 3',5'-adenosine monophosphate.


Metabolism of pyruvate and L-lactate by rat adipose tissue.


A modification of Kerley's method for the microscopic determination of age in human bone.


Measurement of acidity and equilibria in glacial acetic acid with the glass-calomel electrode system.


Effect of polysorbate 80 and of sodium lauryl sulfate on the photodegradation of flavin mononucleotide: a kinetic study with electron spin resonance.


Recombination between guanidine-resistant and dextran sulfate-resistant mutants of type 1 poliovirus. Mixed infection of monkey kidney cells with two mutants of the LSc2ab strain of poliovirus, one resistant to guanidine and the other resistant to both dextran sulfate and 2-(alpha-hydroxybenzyl)-benzimidazole (HBB), yielded progeny in which the number of gua(r)dex(r) particles exceeded by a factor of 7 to 10 the expected number of similar particles occurring through spontaneous mutation; recombination would explain the fairly high excess of doubly mutant particles that was obtained. Scoring of HBB resistance in 50 gua(r)dex(r) clones suggested that, during recombination, resistance to dextran sulfate is not associated with HBB resistance.


Synthesis of ribonucleic acid in cells infected with LSc poliovirus at elevated temperatures. The synthesis of viral ribonucleic acid (RNA) was detected within 2 hr after infection with LSc poliovirus at 35 C. This RNA eluted as a single peak with 0.9 m NaCl on methylated albumin celite columns, was sensitive to ribonuclease, precipitated in the presence of 2 m LiCl, and had an S(20) value at 34 +/- 2 in linear sucrose gradients. When cells were infected at 39 to 40 C, there was also early synthesis of RNA. However, 2 hr after infection this synthesis was drastically inhibited. The absence of net RNA synthesis at 39 to 40 C during the late stages of infection was not caused by rapid degradation of newly formed RNA, since the RNA produced between 1 and 2 hr at 39 to 40 C was still present 3.5 hr after infection. There was a 3 log(10) inhibition in the production of infectious virus when p-fluorophenylalanine was present in the medium at a concentration of 25 mug/ml. This concentration of analogue had little effect upon the production of viral polymerase and viral RNA. Virus grown in the presence of analogue at a concentration of 10 mug/ml exhibited increased heat sensitivity compared to control virus. However, viral polymerase exhibited no change in sensitivity to heat or manganese when cells were exposed to 25 mug of p-fluorophenylalanine per ml during infection. p-Fluorophenylalanine had a relatively selective effect on viral capsid protein but did not reverse the inhibition of synthesis of viral RNA at 39 to 40 C.


Specific effect of guanidine in the programming of poliovirus inhibition of deoxyribonucleic acid synthesis. Inhibition of HeLa cell deoxyribonucleic acid (DNA) synthesis, which occurred by the 4th to 5th hr after infection with poliovirus, could be blocked completely by guanidine only when it was present before the 2nd hr. At the 2nd hr, there was no significant ribonucleic acid (RNA)-replicase activity, and addition of guanidine inhibited all production of virus but allowed 57% of maximal DNA inhibition to develop. Maximum DNA inhibition developed in cells infected for 4 hr in the presence of guanidine when the guanidine was removed for a 10-min interval. RNA-replicase activity was not enzymatically detectable and viral multiplication did not develop in these cells unless the interval without guanidine was extended to 60 min. The interpretation of the data was that the effect of guanidine on viral-induced inhibition of DNA synthesis was distinct and not a consequence of the inhibition of RNA-replicase.


The inhibitory effect of diphtheria toxin on amino acid incorporation by a bacterial cell-free system.


Another look at corticotrophin therapy: clinical and biochemical assessment.


Metabolism of glyceryl 1-14C-trilinoleate in rat testis.


Further studies on the sites of action of clopamide and furosemide.


Cytomegalovirus infection and the postperfusion syndrome. Recognition of primary infections in four patients.


Results of treatment and clinico-electroencephalographic evolution of infantile spasms.


Blood renin levels in various types of arterial hypertension. Preliminary communication.


The effect of ACTH upon patients having regional enteritis.


Medical grand rounds from the University of Alabama Medical Center.


One-electron-transfer reactions in biochemical systems. II. The reaction of free radicals formed in the enzymic oxidation.


Electron transport and phospholipase activity in encysting cells of the ciliate Colpoda steinii.


Proteolipids. V. The activity of lipid cytochrome C.


Inactivation of lyophilized DPNH dehydrogenase from Escherichia coli by oxygen.


Generation of reducing power in chemosynthesis. V. The mechanism of pyridine nucleotide reduction by nitrite in the chemoautotroph Nitrobacter agilis.


Delayed light studies on photosynthetic energy conversion. II. Effect of electron acceptors and phosphorylation cofactors on the millisecond emission from chloroplasts.


Induction of respiratory metabolism in illuminated Chlorella pyrenoidosa and isolated spinach chloroplasts by the addition of vitamin K5.


Energy-linked reactions in digitonin particles from beef-heart mitochondria.


Interaction between NADH and succinate during simultaneous oxidation by non-phosphorylating submitochondrial particles from bovine heart.


Transitory primary aldosteronism-like condition.


Catalase activity of serum and hepatic tissue in experimental murine hepatitis by MHV-3 virus and in acute experimental CCl-4 poisoning.


Cytomegalovirus infection in chronic liver disease.


Small intestinal histochemical and histological changes in ulcerative colitis.


Mechanism of squalene biosynthesis: evidence against the involvement of free nerolidyl pyrophosphate. Several mechanisms that utilize farnesyl pyrophosphate and nerolidyl pyrophosphate as condensing substrates have been postulated for the asymmetric condensation reaction in squalene biosynthesis. Although there is ample evidence that farnesyl pyrophosphate is a substrate for this reaction, there has been no information concerning the role of nerolidyl pyrophosphate. We have made the following observations that demonstrate that nerolidyl pyrophosphate cannot be a free intermediate in squalene biosynthesis. (a) There is no significant interconversion of farnesyl pyrophosphate and nerolidyl pyrophosphate in a squalene-synthesizing system from yeast. (b) Nerolidyl-1-(3)H(2) pyrophosphate is not converted to squalene in the presence or absence of farnesyl pyrophosphate. (c) The addition of unlabeled nerolidyl pyrophosphate to incubation mixtures does not alter the relative loss of alpha-hydrogens from farnesyl pyrophosphate during its conversion to squalene. The synthesis of nerolidyl-1-(3)H(2) pyrophosphate is described. Chromatographic methods for the separation of pyrophosphate esters of triprenols and terpenols are included.