Bovine enteroviruses: apparent correlation between antigenic characters and capacity to grow in the presence of small concentrations of 2-(alpha-hydroxybenzyl)-benzimidazole.


Experimental studies on bacteriocinogeneity in Cl. perfringens type A. 3. Chemical structure of isolated bacteriocines.


Cerebral arterial spasm. 2. Experimental evaluation of mechanical and humoral factors in pathogenesis.


Cerebral arterial spasm. 3. Partial purification and characterization of a spasmogenic substance in feline platelets.


Effect of angiotensin on urine flow and electrolyte excretion in normal and toxemic pregnancy.


Fibro-fatty proliferation of the median nerve.


The effect of atropine and related drugs on the EEG and behaviour.


The effect of acute anemia on the radiosensitivity of V2 rabbit sarcoma.


Case of the month from the AFIP. An exercise in radiologic-pathologic correlation.


Haemagglutinins from marine sponges.

Haemagglutinins from marine sponges.


The neural transmission of herpes simplex virus in mice. Light and electron microscopic findings.


Histologic study of tissue reaction in canaries, and chicken embryos infected with a pox agent, isolated from parakeets.


Comparative metabolism of two vinyl phosphorothionate isomers (thiono phosdrin) by the mouse and the fly.


On the role of microtubules in movement and alignment of nuclei in virus-induced syncytia. Infection of baby hamster kidney (BHK21-F) cells with the parainfluenza virus SV5 causes rapid and extensive cell fusion. Time-lapse cinematography shows that when cells fuse, their nuclei migrate straight to the center of the syncytium at rates of 1-2 micro/min. Nuclei are often arranged in long, tightly packed, parallel rows in syncytia derived from the fibroblastic BHK21-F cells. Polarization microscopy shows birefringent material between and parallel to these rows of nuclei, and electron microscopy shows bundles of cytoplasmic microtubules, approximately 250 A in diameter, and filaments, approximately 80 A in diameter, parallel to and between the rows of nuclei. Colchicine treatment causes disappearance of microtubules from BHK21-F cells and an apparent increase in the number of 80-A filaments. Although colchicine-treated, SV5-infected cells fuse, their nuclei do not migrate or form rows but remain randomly scattered through the syncytial cytoplasm. Incubation at 4 degrees C does not disrupt microtubules in BHK21-F cells. Rows of nuclei have been isolated from SV5-induced syncytia, and the nuclei in them have been found to be intimately associated with microtubules but not with other cytoplasmic structures. These results suggest that microtubules demarcate cytoplasmic channels through which nuclei migrate and that they may also be involved in the mechanism of nuclear movement.


Ovarian lipoid cell tumor: urinary steroid response to adrenocorticotropin and chorionic gonadotropin.


Response of hyperlipemic subjects to carbohydrates, pancreatic hormones and prolonged fasting.


Traumatic hypopituitarism occurring before puberty: survival 35 years untreated.


Adrenocortical function in Stein-Leventhal syndrome.


A case of glucocorticoid-responsive hyperaldosteronism.


Aqueous vasopressin provocative test of anterior pituitary function.


Effects of cyclic 3',5'-adenosine monophosphate and dibutyryl cyclic 3',5'-adenosine monophosphate on basal and stimulated thyroid function.


Inhibitory effect of the beta-sympatolytic drug propranolol on lipolysis induced by noradrenaline ACTH in normal and hyperthyroid rats.


Inhibition of cell growth in vitro by adenosine 3',5'-monophosphate. Adenosine 3',5'-monophosphate, at a concentration of 40 micrograms per milliliter, inhibits the growth of HeLa and strain L cells in culture. The inhibition becomes progressively greater during the incubation of the cells. Adenosine 5'-monophosphate and adenosine, metabolites of adenosine 3',5'-monophosphate, do not affect the growth of either cell culture. This suggests that 3',5'-monophosphate enters the cell without alteration. Dibutyryl-adenosine 3',5'-monophosphate, reported to have a greater activity than adenosine 3'5'-monophosphate on several tissues, inhibited the growth of the cells much less.


Staphylococcal nuclease: size and specificity of the active site. The dissociation constants and standard free energies of complex formation determined with staphylococcal nuclease and a series of 5'-phosphoryloligothymidyl derivatives of increasing chain length suggest that maximum stability is reached with an oligonucleotide containing three nucleotide units. A proposed model of the active site that contains other knowledge of the specificity and the catalytic mechanism of this enzyme postulates the existence of three nonequivalent phosphate binding subsites and a closely related phosphodiester hydrolytic subsite.


Peptides.

Peptides.


Protection against hypertension due to renal allograft rejection by portacaval transposition and renal artery stenosis in dogs.


Central venous pressure and blood volume determinations after alpha-adrenergic blockade in experimental posthemorrhagic shock in dogs.


"Vestigial" enzymes during embryonic development.


Regulatory phenomena in mammalian serine metabolism.


Regulation of gluconeogenesis and glycolysis: studies of mechanisms controlling enzyme activity.


Microfluorometric study of responses of the cytosol to mitochondrial substrates.


Studies on the role of RNA and protein synthesis in the lipolytic action of growth hormone in isolated fat cells.


The redox state of nicotinamide adenine dinucleotide in the cytoplasm and mitochondria of rat liver.


The redox state of nicotinamide adenine dinucleotide in the cytoplasm and mitochondria of rat liver. General discussion.


Nuclear inclusion bodies in vaginal smears.


Studies on the biosynthesis of NAD by a direct method of tracing metabolism in vivo.


Enzymatic changes in fluorescent alveolar macrophages of the lungs of cigarette smokers.


Swine diseases in Japan.

Swine diseases in Japan.


Investigation of the HI titre of Japanese encephalitis of swine on Taiwan, Republic of China.


The incidence and distribution of the different types of the foot-and-mouth disease virus in India.


Regional epizootiology and prophylaxis of foot-and-mouth disease (India).


The eradication of foot-and-mouth disease in Thailand.


Effects of various peptides on aldosterone production of sodium deficient rats.


Epizootiology and prophylaxis of foot-and-mouth disease in Iran.


Oxandrolone effect on growth and bone age in idiopathic growth failure.


Stimulation of growth hormone secretion in monkeys by adrenalin, pitressin and adenosine-3'5'-cyclic monophosphoric acid (3'5'-AMP).


The impaired response to metyrapone in patients taking oestrogen.


Dissociation of stimulatory effects of corticotrophin on adrenal corticosterone and protein synthesis following hypophysectomy.


Microchemical assays of glucose and glucose-6-phosphate in mammalian pancreatic beta-cells.


Acute enzyme histochemical changes in the zona glomerulosa of the rat adrenal cortex. II. The effect of bilateral nephrectomy either alone or followed by peritoneal dialysis with 5 percent glucose.


Tumors of biliary epithelium and ulcerative colitis.


The mechanism of the partial inhibition of fermentation in yeast by nickel ions.


The reaction of pituitary interstitial cell-stimulating hormone with periodate.


The interaction of N-ethylmaleimide and ACTH on isolated fat cells.


Effect of angiotensin on sympathetic nerve activity.


A study of the mechanisms of cutaneous sensory loss in leprosy.


Influence of angiotensin on the hemodynamic response to exercise in normotensive subjects.


Studies on insulin secretion in vitro from isolated islets of the rat pancreas.


Renin-angiotensin-aldosterone system and sodium balance in experimental renal hypertension.


Inhibition by pentobarbital of ether-induced ACTH secretion in the rat.


Inhibition of vasopressin-induced ACTH release from the pituitary by glucocorticoids in vitro.


Effects of glucagon on carbohydrate synthesis and enzyme activity in rat liver.


Dependence of hormonal yield on the collection method for human pituitary glands. II. LH, ACTH and GH.


Time course for corticotrophin release from ectopic pituitary grafts.


Effect of exogenous ACTH on precursor utilization in lemming adrenal-steroidogenesis.


Effect of physical exercise on tissue respiration of endocrine glands.


Some endocrine and behavioural effects of chlordiazepoxide in septal-lesioned rats.


Adrenocorticotrophic hormone and acquisition of a passive avoidance response: a replication and extension.


Succinic dehydrogenase and cytochrome oxidase of mitochondria of chick liver, heart, and skeletal muscle during embryonic development.


Evaluation of a synthetic 25 amino acid corticotrophin in normal man.


The form of sodium-calcium competition at the frog myoneural junction. The times required for a steady rate of miniature end-plate potential discharge to be reached in response to changes in extracellular [K(+)], [Na(+)], and [Ca(++)] have been measured. In the presence of 15 mM KCl, Ca(++) raises and Na(+) lowers the steady-state mepp frequency; but the depressive effect on Na(+) is not specific: Li(+) can replace Na(+) to a large extent. Mepp frequency has been found to depend on the ratio of [Ca(o) (++)]/[Na(o) (+)]. It is assumed that in the steady state, intracellular sodium will change when extracellular sodium is changed. Because both intracellular and extracellular sodium at motor nerve endings affect acetylcholine release, it is proposed that mepp frequency depends on the ratio [Ca(o)] [Na(i)](2)./[Na(o)](2) Two models are proposed. Firstly, to account for the action of sodium and calcium a carrier is postulated for which Ca(++) and Na(+) compete. The carrier determines a maximum level of intracellular Ca(++) far lower than predicted by the Nernst equation for Ca. Secondly, to account for activation of acetylcholine release by a small influx of Ca(++), the ions are presumed to enter the nerve ending in a two stage process through a small intermediate compartment and to act on the acetylcholine release site in this region rather than after entering directly into the cell.


The effects of cardiac glycosides and their interaction with catecholamines on glycolysis and glycogenolysis in skeletal muscle.


The circulatory effects of catecholamines and ouabain in glucocorticoid-deficient animals.


Conversion of angiotensin I to angiotensin II by cell-free extracts of dog lung.


Selective retention of corticosterone by limbic structures in rat brain.


Antineoplastic activity of aflatoxin B1.


Histochemical method for demonstrating Na-K-ATPase and K-phosphatase at the fine structural level.


An analysis of some inhibitory mechanisms in the spinal cord of the frog (Xenopus laevis).


Secobarbital and nocturnal physiological patterns.


The relationship of glucocorticoids to the early changes of liver triglycerides content after partial hepatectomy in rats.


Studies on the fixation and development of cellular transformation by Rous sarcoma virus.


Evidence that gene 56 of bacteriophage T4 is a structural gene for deoxycytidine triphosphatase.


In vitro replication of mouse sarcoma virus (Moloney strain) in bovine embryo skin cells.


A critical evaluation of the contamination, by lysosomes, of preparations of outer membrane of mitochondria.


An epidithiapiperazinedione antiviral agent from Aspergillus terreus.


Localization of phospholipase A2 in outer membrane of mitochondria.


Fructose 1,6-diphosphatase from rabbit liver. XI. Relation between the adenosine 5'-monophosphate binding and the allosteric inhibition.


Early effects of gibberellic acid on barley aleurone layers.


Enzymatic phosphorylation of serine by inorganic pyrophosphate.


Negative cooperativity in enzyme action. The binding of diphosphopyridine nucleotide to glyceraldehyde 3-phosphate dehydrogenase.


Interaction between energy charge and metabolite modulation in the regulation of enzymes of amphibolic sequences. Phosphofructokinase and pyruvate dehydrogenase.


Adenosine 5'-phosphorothioate. A nucleotide analog that is a substrate, competitive inhibitor, or regulator of some enzymes that interact with adenosine 5'-phosphate.


Purification and properties of sugar-non-specific nuclease in pupa of silkworm, Bombyx mori Linné.


Factors affecting the cation requirement of a halophilic NADH dehydrogenase.


Reactions of DPN+ and pyruvate.

Reactions of DPN+ and pyruvate.


Plasma levels of immunoreactive corticotrophin in patients with Cushing's syndrome. Plasma levels of immunoreactive corticotrophin (A.C.T.H.) have been determined in 56 patients with Cushing's syndrome by means of a homologous radioimmunoassay. In untreated Cushing's disease (bilateral adrenal hyperplasia due to excessive A.C.T.H. secretion from the pituitary) plasma values ranged from 40 to 200 mumug./ml., between 8 and 10 a.m., compared with a range in normal subjects of 12 to 60 mumug./ml. Considerably raised levels, often above 2,000 mumug./ml., were found in patients with Cushing's disease after bilateral adrenalectomy. A.C.T.H. concentrations were usually higher in patients with bilateral adrenal hyperplasia associated with ectopic A.C.T.H. production than in patients with untreated Cushing's disease; whereas plasma A.C.T.H. was undetectable in the presence of an adrenocortical tumour. All patients with Cushing's syndrome failed to show the normal circadian rhythm of circulating A.C.T.H. levels.


A method for the determination of estriol in urine during pregnancy.


A study of the determination of lactic dehydrogenase isoenzymes by means of agar gell and strach-agar gel zymograms.


Effects of "dream deprivation" on dream content: an attempted cross-night replication.


Nephrolithiasis in inflammatory bowel disease.


Canine submandibular-gland hyaluronidase. Identification and subcellular distribution. 1. Submandibular glands from four species of mammal have been shown to contain a hyaluronidase active at acid pH; glands from dog and cat had a much higher content of this enzyme than has been found in other sources. 2. Product formation from hyaluronate after 24hr. incubation was almost the same as with testicular hyaluronidase, indicating that the enzyme is an endo-poly-beta-hexosaminidase. 3. When submandibular-gland homogenates were fractionated by the scheme developed for liver by de Duve, Pressman, Gianetto, Wattiaux & Appelmans (1955), all the enzymes assayed, except cytochrome c oxidase, were found to occur partly in the soluble fraction and partly in the particulate fractions. Among the particular fractions, the highest specific activity was found in the heavy-mitochondrial fraction for cytochrome c oxidase, in the microsomal fraction for alkaline phosphatase and in the light-mitochondrial fraction for acid phosphatase, beta-N-acetylhexosaminidase and acid-active hyaluronidase. 4. Release of the enzyme activity from the sedimentable fractions occurred in 0.1% Triton X-100 or after high-speed homogenization. 5. Stimulation of dogs by pilocarpine was found to decrease the hyaluronidase content of the submandibular gland by 5% and to cause the occurrence of a corresponding amount of acid-active hyaluronidase in the submandibular saliva. 6. The results are discussed in relation to the subcellular localization of hyaluronidase.


Characterization of N4,O2'-dimethylcytidine, a rare nucleoside constituent of Escherichia coli 16-S RNA.


The modification of nucleosides and nucleotides. X. Evidence for the important role of inosine residue in codon recognition of yeast alanine tRNA.


RNA fractions in normal rat liver and Morris 5123D hepatoma.


Studies on tissue specificities of nuclear and ribosomal RNA using the DNAxRNA hybridization technique.


Purification of nucleoside triphosphatases from pea seedling ribosomes.


RNA with amino acid-acceptor activity isolated from an oncogenic virus.


Sucrose density-gradient analysis of Euglena gracilis RNA isolated with macaloid.


A phosphate activated glutaminase in rat liver different from that in kidney and other tissues.


The fate of parenterally injected 14C-hyaluronidase.


Purification of collagenase. IV. Separation and identification of clostridial collagenases by disc electrophoresis on polyacrylamide gel.


Pseudogout, hyperparathyroidism, and Carpal-Tunnel syndrome. A case report.


Cytomegalic inclusion disease of the inner ear.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 52-1968.


Input-output relation of the vestibular system during sleep and wakefulness.


Bronchiolar carcinoma of the lung.

Bronchiolar carcinoma of the lung.


Adenosine monophosphates and phosphorylase activity in frog stomachs.


Adenosine monophosphates and glycogenolysis in frog gastric mucosa.


Converting enzyme in CC14-treated renal hypertension.


Inhibition of angiotensin and its protection by a cofactor in blood.


Interrelationship of physical factors affecting sodium reabsorption in the dog.


Effects of angiotensin on stroke volume and regional blood flow and resistance.


Partial purification and properties of nicotinamide-adenine dinucleotide nucleosidase and interaction between enzyme and its protein inhibitor.


Inhibitory effect of dehydroacetic acid on induction of hepatoma in rats fed 4-(dimethylamino) azobenzene.


Cellular oxidation-reduction stae of some NAD-linked reactions in diabetic rat liver, and the effect of coenzyme Q administration.


Brain tumors induced with Rous sarcoma virus, Schmidt-Ruppin strain. I. Induction of brain tumors in adult mice with Rous chicken sarcoma cells.


A preliminary report on infection of the lizard, Takydromus tachydromoides, with Japanese encephalitis virus.


Isolation of Japanese encephalitis virus from mosquitoes by means of the plaque formation method with chick embryo fibroblast monolayers.


Studies on poliovirus inhibitors in sera of domestic animals. 3. A comparison of physico-chemical properties of poliovirus inhibitors and specific antibodies.


A simple method for the serum neutralization with Japanese encephalitis (JE) virus utilizing plaque inhibition by agar-diffused antibody.


Effects of dibutyryl cyclic 3',5'-adenosine monophosphate on the renal function.


Distribution volume and metabolic clearance rate of renin in anesthetized nephrectomized dogs.


Statistical analysis of data from countercurrent distribution. I. Method for estimation of quantity and partition coefficient of each compound in the distribution.


Statistical analysis of data from countercurrent distribution. II. Some applications and limitations of the method.


A method for quantitative determination of phosphonate phosphorus in the presence of organic anc inorganic phosphates.


The use of specific macromolecular complexing agents for enzyme purification: beef heart dehydrogenase.


A study of the physical properties of the immunizing antigen of foot-and-mouth disease virus and the effect of various inactivating agents on its structure.


Adenyl cyclase activity in the chronically denervated cat heart.


Neuro-ophthalmology.

Neuro-ophthalmology.


Adenyl cyclase in plasma membrane preparations of sea urchin eggs and its increase in activity after fertilization.


Rates of exchange of isotopic hydrogen between reduced substrates of coupled NAD-dependant dehydrogenases.


The effect of cyclic 3',5'-AMP on catabolite repression of beta-galactosidase synthesis in Escherichia coli.


Solubilization of bound lactate dehydrogenase by NADH in homogenates of trout skeletal muscle as a function of tissue concentration.


Endocrine ophthalmopathy.

Endocrine ophthalmopathy.


Sequence determination in nucleic acids.


Oxidative phosphorylation.

Oxidative phosphorylation.


Absence of effects of prostaglandins E1 and E2 on ganglionic transmission.


Effects of catecholamines on the gastrocnemius muscle of the domestic fowl.


Formation and identification of 3-keto-5-aminohexanoic acid, a probable intermediate in lysine fermentation.


Inhibition of enzyme activities by free fatty acids.


Diacyl myoinositol monomannoside from Propionibacterium shermanii.


Effect of age on oxidative phosphorylation in the rat.


Replication and complementation of human adenoviruses and simian papovavirus at an elevated temperature. The simian papovavirus SV40 replicated as well in simian cells incubated at 41 C as in cells incubated at 37 C, although the latent period was shortened at the elevated temperature. Human adenoviruses differed in their responses to the elevated temperature. Some serotypes, such as 3, 4, 5, 7, 8, 16, and 21, replicated as well, or almost as efficiently, in human cells incubated at 41 C as in cells incubated at 37 C, whereas with other serotypes, such as 1, 2, 6, 12, and 14, maximal yields in cultures incubated at 41 C were much lower than the yields from companion cultures incubated at 37 C. This difference was also detected in simian cells co-infected with SV40 and a human adenovirus; maximal complementation occurred with some serotypes at the elevated temperature but not with other serotypes. The degree of complementation observed in the simian cells at 41 C was directly correlated with the ability of the adenovirus to replicate at 41 C in human cells. Therefore, the capacity of SV40 to serve as a helper virus is not affected by the elevated temperature, showing that the complementation event supplied by the simian virus is heat-stable between 37 and 41 C. Maximal complementation appeared to depend upon a characteristic present in the adenovirus genome.


Incorporation of iododeoxyuridine into cellular deoxyribonucleic acid synthesized after infection with simian virus 40. Primary monkey kidney cells (Cerocpithecus aethiops) in the stationary phase of growth were labeled with (14)C-thymidine for 24 hr prior to infection with simian virus 40 (strain 777). (3)H-deoxyadenosine and 5-iodo-2'-deoxyuridine (IUdR) were added to some of the cultures 24, 48, or 72 hr after infection; 24 hr later the deoxyribonucleic acid (DNA) was extracted from these cultures and centrifuged in a CsCl density gradient. The portion of DNA which had become heavier because of incorporation of IUdR could be seen as a second peak in the sedimentation profile. This peak contained (14)C as well as (3)H activity. The possibility that the (14)C-labeled cellular DNA might be degraded and used for the synthesis of viral DNA could be excluded. On the basis of these results, it must be assumed that the infection of monkey kidney cells with simian virus 40 induces the synthesis of cellular DNA.


Infective substructures of Sendai virus from infected Ehrlich ascites tumor cells. Increase of infectivity for embryonated eggs was observed in Ehrlich ascites tumor cells after intraperitoneal inoculation of Sendai virus into tumor-bearing mice. Virus-induced actinomycin-resistant ribonucleic acid consisting of 14S, 18S, 22S, 35S, and 48S was synthesized, and S antigen was produced in infected cells. The infectivity was suggested to be due to viral ribonucleoprotein for the following reasons: (i) the infectivity was unaffected by V antiserum but was abolished by whole hyperimmune serum, (ii) the infectivity was resistant to ribonuclease, (iii) virus particles were found neither in cells nor on red blood cell stroma treated with cellular extracts, (iv) structures similar to Sendai virus ribonucleoprotein with a maximal length of 10,500 A were observed in cellular extracts.


Factors controlling the interconversion of enzyme-substrate compounds of pig heart lactate dehydrogenase.


Some aspects of the mechanisms involved in adrenergic neurotransmission.


The antiviral activity of 3,4-dihydro-1-isoquinolineacetamide hydrochloride in vitro, in ovo, and in small laboratory animals.


Preservation of infectious cytomegalovirus.


Nucleotide accumulations in Escherichia coli infected with some bacteriophage T4 amber mutants.


The proteins specified by herpes simplex virus. I. Time of synthesis, transfer into nuclei, and properties of proteins made in productively infected cells.


Shared group-specific antigen of murine and feline leukemia viruses.


Control of DNA synthesis in cells infected with polyoma virus.


Observations on polyoma virus filaments.


Comparative electron micrographs of sowthistle yellow vein and vesicular stomatitis viruses.


Structural relationships between the RNA of mammary tumor virus and those of other RNA tumor viruses.


Absence of homology between polyoma or SV40 viral DNA and mitochondrial DNA from virus-induced tumors.


Perihepatic hematoma. A benign condition simulating malignancy on the hepatic photoscan.


Granular cell myoblastoma of duodenum. A review of literature and case report.


Defectiveness of interferon production and of rubella virus interference in a line of African green monkey kidney cells (Vero). Vero cells, a line of African green monkey kidney cells, failed to produce interferon when infected with Newcastle disease, Sendai, Sindbis, and rubella viruses, although the cells were sensitive to interferon. Further, infection of Vero cells with rubella virus did not result in interference with the replication of echovirus 11, Newcastle disease virus, or vesicular stomatitis virus, even in cultures where virtually every cell was infected with rubella virus. Under the same conditions, BSC-1 cells and other cells of primate origin produced interferon and showed rubella virus interference. The results indicate that the presence of rubella virus in the cell does not in itself exclude multiplication of other viruses and that rubella virus interference appears to be linked to the capability of the cell to produce interferon.


Herpes simplex virus products in productive and abortive infection. 3. Differentiation of infectious virus derived from nucleus and cytoplasm with respect to stability and size. Approximately 67% of infectivity is associated with the nucleus 8 hr after productive infection of HEp-2 cells with herpes simplex virus. Comparison of nuclear and cytoplasmic infectious virus and macromolecular aggregates labeled with (3)H-thymidine or with (3)H-choline revealed the following. (i) Cytoplasmic infectious virus and macromolecular aggregates banded in CsCl at a density corresponding to enveloped nucleocapsids. The virus was relatively stable; there was only 50% loss of infectivity and only 16% of the virions became disaggregated. (ii) Nuclear macromolecular aggregates banded in CsCl solution at a density corresponding to unenveloped nucleocapsids and, moreover, both the infectious virus and aggregates were highly unstable. (iii) In sucrose density gradients, the nuclear macromolecular aggregates and infectivity sedimented as a single band and migrated more slowly than the corresponding cytoplasmic material. (iv) The infectivity of nuclear and cytoplasmic virus is readily inactivated by digestion with phospholipase C and with pronase. We conclude the following. (i) Cytoplasmic virus consists of enveloped nucleocapsids. (ii) Nuclear virus consists of nucleocapsids covered with lipid or partially enveloped. (iii) The molecular integrity of viral lipids is essential for infectivity. (iv) The envelope protects the nucleocapsid and accelerates adsorption to cells; it is not, however, inherently essential for infectivity.


Chromosomal and autoradiographic studies of cells infected with herpes simplex virus. The induction of chromosomal aberrations by herpes simplex virus (HSV) and the interaction between viral deoxyribonucleic acid (DNA) and chromosomes have been studied (i) by infection of the BHK-21 line of hamster kidney cells at multiplicities ranging from 0.5 to 100 followed by 1-hr pulses of (3)H-thymidine preceding preparation at varying intervals of metaphases and autoradiography, and (ii) by use of (3)H-thymidine-labeled HSV for infection, chromosomal, and autoradiographic analyses at intervals thereafter. The results revealed that (i) chromosomal lesions develop prior to, and thus are independent of, viral DNA synthesis; (ii) HSV is capable of replicating in cells during the late G-2 period or in metaphase; (iii) most of the viral DNA remains unassociated with chromosomes and is not detectable at sites of chromosomal lesions; (iv) the capacity of the virus to cause chromosomal aberrations is four times less sensitive to inactivation by ultraviolet (UV) irradiation than its infectious property; and (v) after large doses of UV, invasion of the nuclei by the irradiated virus is reduced. These observations indicate that the chromosomal lesions induced by HSV result most likely from action of an early enzyme under control of the viral genome. This explanation is proposed also for the effects of adenovirus type 12 on chromosomes.


Structure and development of viruses as observed in the electron microscope. IX. Entry of parainfluenza I (Sendai) virus. After attachment, the uncoating of Sendai virus, which was accompanied by dissolution of the plasma membrane and fusion of virus to cell, proceeded quickly. Nucleoprotein filaments were found at stages of transit from virus to cytoplasm.


Effects of x-irradiated aqueous solutions on vesicular stomatitis virus. Infectivity of vesicular stomatitis virus was inactivated by suspending the virus in aqueous salt solutions that had previously been subjected to X-irradiation. This viral inactivation did not appear to be attributable to hydrogen peroxide or to long-lived free radicals generated by X-irradiation of the salt solutions. Indirect evidence suggested that the deleterious effects on the virus were caused by chemical species resulting from interaction of free water molecules with anions in the solution. Inactivation of infectivity was associated with distortion of the outer layer of the virion, as determined by electron microscopy, and with consequent impairment of adsorption to otherwise susceptible cells.


Electron microscopic study of the morphogenesis of vesicular stomatitis virus. Except for the rate, vesicular stomatitis virus (VSV) grows as well at 25 C as at 37 C in primary chick embryo fibroblast cells and in a pig kidney cell line [PK(H13)]. Maximal yields were reached at about 28 hr at 25 C and 10 hr at 37 C in these cells. Morphogenesis, as observed by electron microscopy, was similar at the two temperatures. The main feature was accumulation of virus in intracytoplasmic vacuoles. Mode of release of VSV has been controversial; both budding (as displayed by myxoviruses) and maturation at membranes of cytoplasmic vacuoles (as with arboviruses) have been claimed. Our observations support the latter view, and the apparent dichotomy in interpretation is discussed.


Electron microscopy of herpes simplex virus. 3. Effect of hydroxyurea. The effect of hydroxyurea on the development of herpes virus is mediated through its inhibitory action on deoxyribonucleic acid (DNA) synthesis. Concentrations of the drug that suppress the production of infectious virus cause typical developmental anomalies: failure in formation of the normally dense cores or "complete" viral particles, and either faulty or no envelopment of viral capsids by membranes. The synthesis of viral capsids and virus-stimulated nuclear and cytoplasmic membranes, however, is not interrupted. Combining these results with those of time sequence experiments, the following hypotheses can be presented regarding viral development. Protein synthesis, which is characterized by capsids enclosing cores of low density, precedes DNA synthesis, which is characterized by the appearance of dense cores. Capsids with dense cores are selectively transported to the cytoplasm. Envelopment generally takes place as capsids pass from the nucleus to the cytoplasm. The process of envelopment is also selective, with the result that the majority of particles that have an envelope contain a full quota of DNA.


Electron microscopy of herpes simplex virus. IV. Studies with ferritin-conjugated antibodies. Small aggregates of viral antigen were encountered in the nuclear matrix. The capsids did not tag with antibodies specific for the virus or for the host cell. This observation remains unexplained. Nuclear and cytoplasmic membranes, as well as the envelope of the virus, reacted with both types of antibodies and appear, therefore, to contain host cell and viral protein. Large amounts of viral antigen are synthesized within the cytoplasm. This antigen was either diffusely spread or localized at the surface of membranes. The surface of infected cells contains viral antigen, which accumulates as infection progresses. At circumscribed sites, the cell wall becomes altered antigenically and structurally so as to resemble the envelope of the virus. Hypotheses are presented regarding the manner in which cell fusion occurs.


Studies on streaming. I. Inhibition of protoplasmic streaming and cytokinesis of Chaos chaos by adenosine triphosphate and reversal by magnesium and calcium ions. In Chaos chaos streaming, motility, and cytokinesis were inhibited nearly 100% for several hours by 2.5-5 mM sodium adenosine triphosphate (ATP)(1) added to culture fluid. All three effects were completely prevented by the addition of equimolar Mg(++) or Ca(++) ions but not Na(+) to the ATP/culture fluid solution. The effects of ATP were not reproduced by EDTA, EGTA, colchicine, or AMP. Sodium pyrophosphate produced about 50% inhibition at 5 mM. Studies with (14)C-ATP showed that 5 x 10(-5) to 5 x 10(-4) mmole of ATP was firmly associated with each milliliter of packed cells after an hour's incubation at 24 degrees C. Labeling studies also showed that prevention of the ATP effects by Mg(++) ions was not due to a decrease in the amount of ATP associated with the cells.


Genetic variance in skeletal and dental maturity status of Caucasian dizygotic twins.


3. Effect of morphine and nalorphine on brain phospholipid metabolism.


An autopsy case of stunted growth with fatty liver cirrhosis, hepatoma, hypogonadism, osteoporosis and lipid pneumonia.


Association of thyroid carcinoma and chemodectoma.


Gustatory sweating.

Gustatory sweating.


Cytological changes of Escherichia coli caused by polymyxin E.


Production of interferon-like inhibitor induced by extract from Rous sarcoma. I. Inhibition of Rous sarcoma virus infection and properties of the inhibitor produced.


Production of interferon-like inhibitor induced by extract from Rous sarcoma. II. Properties of the inducer in the extract.


Toxicology and carcinogenic action of pyrrolizidine alkaloids.


Synergism between cyclopropenoid fatty acids and chemical carcinogens in rainbow trout (Salmo gairdneri).


Relation of the centrally evoked pressor effect of angiotensin II to the calcium ion.


Periodate oxidation of acid polysaccharides inhibition by the electrostatic field of the substrate.


Differences in maturity, physical, and motor traits for boys of high, average, and low gross and relative strength.


Strokes-modern treatment.

Strokes-modern treatment.


Serum lipid studies in multiple sclerosis.


Enzyme histochemistry of the cerebral cortex of squirrel monkey and rat.


The effect of cortisone and ACTH on behaviour of beta-glucuronidase and beta-galactosidase in stomach mucosa of white rat.


Isotope scanning of brain tumors using 99mTc.


Effect of angiotensin on the dog kidney.


Ovarian tumors investigated by histochemical and enzyme histochemical methods.


Review of diagnostic audiometry.

Review of diagnostic audiometry.


Incidence and histochemical investigation of enzymatically active cells in stroma of ovarian tumors.


Afferent nerve fibers in the external branch of the superior larygneal nerve in the cat.


Otolaryngological aspects of thyroid cancer.


Facial paralysis in cephalic herpes zoster.


Anaphylaxis and skin homograft rejection: effect of simultaneous splenectomy and thymectomy in rats and guinea pigs.


Motor-point biopsies in carcinomatous neuropathy.


A new ocular syndrome.

A new ocular syndrome.


Trimethoprim.

Trimethoprim.


Acquired cytomegalovirus infection.

Acquired cytomegalovirus infection.


Idiopathic facial palsy.

Idiopathic facial palsy.


Herpes simplex cervicitis.

Herpes simplex cervicitis.


Osmotic and ionic regulation in gold fish following removal of the corpuscles of Stannius or the pituitary gland.


Endocrine factors in the development of transplantable adrenal cortical tumors. I. Inhibition of the growth rate by corticotropin (ACTH).


Immunologic studies on Graffi-virus-infected spontaenous Gross leukemia of AKR mice.


The differential action of neonatal thymectomy in mice infected with Murine Sarcoma Virus-Harvey (MSV-H).


Polyoma virus replication in ascitic cells.


Susceptibility of Praomys (Mastomys) natalensis to the Murine Sarcoma Virus-Harvey (MSV-H).


The basis of the tumorigenicity of BHK 21 cells.


Relationships of chromosomal and pathological findings in Rous sarcoma virus-induced tumours in the mouse.


Contribution to the diagnosis and treatment of tumors of the glomus jugulare.


Periodic postoperative mammography.

Periodic postoperative mammography.


Studies on production and purification of cytochrome C of Candida krusei and its properties.


Mode of action of piericidin A on the phosphorylating respiratory chains.


Isolation and characterization of a glycopeptide from a mucinous carcinoma in a human stomach.


Production of the inducer for macrophage and granulocyte colonies by leukemic cells.


Lipids of cancer tissues. 3. Glycolipids of human hepatoma tissue.


Further study on the faster running glycolipid in brain.


The ultrastructure of the nuclei and the behaviour of the sex chromosomes of human spermatogonia.


Selective enhancement of glycolytic activity by incubation of yeast cells in sugars and polyols.


The value of radiation therapy for pituitary and parapituitary tumours.


Arrhenoblastoma of the ovary in an adolescent.


The mechanism of supersensitivity to norepinephrine induced by cocaine in rat isolated vas deferens.


DOPA-decarboxylase activity in sciatic nerves of the rat after constriction.


Platelet lipids. II. In vitro incorporation of 1-14-C palmitic acid into lipid fractions of rabbit platelets.


Depression of spinal motoneurones by noradrenaline, 5-hydroxytryptamine and histamine.


Mitochondrial enzyme activity in "senescent" and virus-transformed human fibroblasts.


Nonchromaffin paraganglioma of the orbit.


Evaluation of the increased risk of gastrointestinal bleeding following intracranial surgery in patients receiving high steroid dosages in the immediate postoperative period.


Relationship of route of inoculation and nature of toxin preparation to bioassay of Clostridium perfringens alpha-toxin in mice. The toxicity of Clostridium perfringens was determined in mice utilizing three different routes of inoculation. There was marked variation in the minimum lethal dose with the different routes; the largest amount was required for the intracutaneous route, less for the intravenous route, and least with the intraperitoneal route. The relationship of toxicity to the lecithinase content of different toxin preparations was assayed. It was found that the toxicity of toxin preparations in different states of purification was not correlated with their lecithinase content.


Cytochrome C552 in Agrobacterium tumefaciens.


Purification and properties of cytochrome c-553 and cytochrome B-560 from Tetrahymena pyriformis.


Multiplication in and transmission by Aedes aegypti of vesicular stomatitis virus.


Facial myokymia.

Facial myokymia.


A program of resocialization for a long-term regressed schizophrenic patient.


A rational approach to short-term counseling.


Infection of adult mouse macrophages in vitro with cytomegalovirus.


"Treatment-resistance" to idoxuridine in herpetic keratitis.


Planning playgrounds for the handicapped.


Development of delayed dermal hypersensitivity in guinea pigs immunized with inactivated respiratory syncytial virus vaccines.


Treatment drop-outs and the role of the crisis therapist.


Histochemical studies on phosphatase and arylsulphatase activity in human carious dentine.


Studies on Sendai virus cell fusion factor.


Movigenic intervention: an expanded dimension.


Nursing apparel and psychiatric patients: a comparison of uniforms and street clothes.


Flow responses to angiotensin in innervated and denervated kidneys.


International trends.

International trends.


Physiologic and pathologic changes in acrylamide neuropathy.


Live vaccines against influenza, adenoviruses, measles, and mumps.


The "generic equivalency" game.

The "generic equivalency" game.


Alloxan and lactic acid dehydrogenase activity of the cornea.


Orbital malignant non-chromaffin paraganglioma. Alveolar soft tissue sarcoma.


Effects of magnesium and calcium on neurones in the central nervous system.


A fluorescent spot test for creatine kinase.


Perchloric acid interference in sodium and potassium determinations by atomic absorption.


Localization of cell-bound penicillinase in Bacillus licheniformis. When protoplasts are prepared from Bacillus licheniformis (strain 749/C, constitutive for penicillinase), approximately 60% of the cell-bound penicillinase is released. The remainder is retained by the protoplast and cannot be removed by washing. This release is specific, in that less than 7% of the cellular reduced nicotinamide adenine dinucleotide (NADH) dehydrogenase and alpha-glucosidase is liberated by the treatment. The freed penicillinase is excluded from G-200 Sephadex, and it is partially sedimented with a force of 65,000 x g for 20 hr. It is probably attached to characteristic tubular and vesicular structures with single-layered membranes that are comparable to structures previously described in intact penicillinase-forming cells. The specific activity of the organelle is more than six times that of twice washed peripheral membrane; furthermore, about 8% of the protein of the structure is penicillinase. At substrate concentrations (benzylpenicillin) of about one-fifth the K(m) value, whole cells show a slight permeability restriction, although this does not occur in isolated particles and protoplasts.


Degradation of collagen by a human granulocyte collagenolytic system. This report suggests a mechanism for collagen degradation mediated by human granulocytic leukocytes. A specific collagenase, which is extractable from human granulocytes, has been partially purified by DEAE chromatography. This collagenolytic enzyme is operative at physiological pH and is inhibited by EDTA, cysteine, and reduced glutathione but not by human serum. The enzyme cleaves the collagen molecule into two specific products, without loss of helical conformation. Electron micrographs of segment long spacing aggregates indicate that the cleavage occurs one-quarter of the length from the carboxy terminal end of the molecule. Experiments with crude extracts from granulocytes suggest that the specific products of granulocyte collagenase activity are then degraded by other proteases present in the human granulocyte.


Activation of Hageman factor by collagen. Purified acid-soluble and insoluble human collagen accelerated the clotting of plateletpoor plasma in silicone-treated tubes. The clot-promoting effect did not appear to be due to thromboplastic activity since the collagen preparations did not activate factor X in the presence of factor VII and calcium. Instead, collagen appeared to accelerate clotting by activating Hageman factor (factor XII) on the basis of the following findings: collagen increased the clot-promoting activity of partially purified Hageman factor but exerted no further effect in the presence of kaolin, a known activator of Hageman factor; clot-promoting eluates were obtained from collagen exposed to normal, hemophilic, or PTC-deficient plasma but not from collagen exposed to Hageman or PTA-deficient plasma. The collagen molecule itself appeared to be required for the clot-promoting activity since digestion with collagenase or thermal denaturation at pH 2.5 (about 35 degrees C) resulted in very marked reduction in clot-promoting activity. Since thermal denaturation is associated with transformation of collagen structure from triple helical to random coil form, it is suggested that the native form of collagen is essential for the ability to activate Hageman factor. Blockage of the free amino groups by treatment with nitrous acid or dinitrofluorobenzene only slightly reduced the clot-promoting activity of collagen. In contrast, since addition of cationic proteins to collagen markedly reduced pro-coagulant activity it is suggested that negatively charged sites on the collagen molecule are critical for Hageman factor activation. This suggestion is supported by the finding that pepsin treatment of collagen, which removes the predominantly negatively charged telopeptides, results in significant decrease in coagulant activity. Esterification of collagen, which neutralizes 80-90% of the free carboxyl groups, reduced coagulant activity by over 90% and it is suggested that the free carboxyl groups of glutamic and aspartic acids provide the negatively charged sites critical for Hageman factor activation.


Vascular responses to norepinephrine, angiotensin, vasopressin and serotonin.


Incorporation of orthophosphate-32P into erythrocyte phospholipids in normal subjects and in patients with hereditary spherocytosis. The in vitro incorporation of inorganic (32)P into erythrocyte phospholipids has been studied in normal subjects and in splenectomized patients with hereditary spherocytosis (HS). Phosphatidic acid (PA) was the only lipid measurably labeled in both kinds of cells. The actual turnover rate of PA phosphate was determined by simultaneously isolating inorganic phosphate (P(i)) and adenosine triphosphate (ATP) and determining their specific activities. This turnover is very small: 1.3 mumoles P/liter of erythrocytes per hr in normal cells and 4.0 mumoles P in HS erythrocytes when either ATP or cellular P(i) is considered the immediate precursor. This value represents less than 0.1% of the total membrane lipid phosphate. Incorporation of added (32)P(i) into the other phosphatides, including phosphatidyl serine, was essentially zero in both kinds of cells. The effects of stimulation and inhibition of active cation transport, metabolic depletion, and extracellular phosphate concentration on both the degree of labeling and the actual turnover of PA phosphate were studied. In any given experiment, the degree of labeling of PA depended on the specific activities of the other intracellular phosphates (P(i) and ATP). The actual turnover rate of PA phosphate, however, did not vary with active transport or metabolic depletion. The greater turnover of PA phosphate in HS erythrocytes may be due to the somewhat younger age of these cells. The results suggest that the very low turnover of PA phosphate in erythrocytes is mediated by nonspecific enzyme reactions, and that it is quantitatively insignificant in both normal and HS erythrocytes. The results also emphasize the importance of measuring intracellular phosphate precursors in any study evaluating cellular phospholipid turnover from added (32)P(i).


Studies on collagenase from rheumatoid synovium in tissue culture. Fragments of synovium from patients with rheumatoid arthritis survive in defined tissue culture medium in the absence of added serum and, after 3-4 days, release into the medium enzyme capable of degrading undenatured collagen. Maximal activity is observed at pH 7-9 but the enzyme is inactive at pH 5. At temperatures of 20 degrees and 27 degrees C, collagen molecules in solution are cleaved into 3/4 and 1/4 length fragments with minimal loss of negative optical rotation, but with loss in specific viscosity of approximately 60%. Above 30 degrees C the fragments begin to denature and denaturation is complete at 37 degrees C. If the enzyme is not inhibited at this stage the large fragments are broken down further to polypeptides of low molecular weight. Reconstituted collagen fibrils and native fibers at 37 degrees C are cleaved to the low molecular weight fragments, although the fibrils are resistant to breakdown at lower temperatures (20 degrees -27 degrees C). It is proposed that the production of such an enzyme by inflamed and proliferating rheumatoid synovium may be responsible for some of the destruction of collagenous structures that accompanies rheumatoid arthritis.


Effect of heat treatment and storage temperature on phosphatase reactivation in butter.


Radioimmunoassay of ACTH in plasma.

Radioimmunoassay of ACTH in plasma. Techniques are described in detail for a radioimmunoassay of plasma adrenocorticotropin (ACTH) that is capable of detecting hormone in unextracted normal human plasma at 1:5 dilution under the conditions described. The sensitivity of the assay is at the level of 1 mumug/ml (equivalent to 0.014 mU/100 ml). In normal subjects ACTH concentrations averaged 22 mumug/ml (equivalent to 0.308 mU/100 ml) plasma at 8-10 a.m. In a smaller group the concentrations averaged 9.6 mumug/ml (equivalent to 0.134 mU/100 ml) at 10-11 p.m. Although a circadian rhythm in normal subjects was not always well marked throughout the daytime hours, plasma ACTH usually fell to its lowest value in the late evening. In hospital patients who were not acutely ill, concentrations were infrequently above 100 mumug/ml in the morning and usually fell to significantly lower levels in the late evening. Severely ill hospital patients occasionally exhibited a.m. concentrations above 200 mumug/ml. In a group of subjects showing frequent spiking of plasma 17-OHCS concentrations throughout the day parallel spiking of plasma ACTH as well was generally observed.Metyrapone produced marked increases in plasma ACTH within 24 hr in all cases and generally within 3-6 hr except when started late in the day. Dexamethasone brought about a persistent reduction in plasma ACTH in a patient under continued treatment with metyrapone.Hypoglycemia, electroshock, surgery under general anesthesia, histalog and vasopressin administration were usually followed by significant increases in plasma ACTH concentration. Prior administration of dexamethasone blocked the response to hypoglycemia. Marked elevations in plasma ACTH were observed in patients with adrenal insufficiency off steroid therapy, in Cushing's disease after adrenalectomy even in the presence of persistent hypercortisolemia, and in some untreated patients with Cushing's disease. Umbilical cord blood contained higher plasma ACTH concentrations than maternal blood at delivery in seven of eight cases. After suppression of ACTH secretion by dexamethasone or cortisol. ACTH disappeared from plasma with half-times ranging from 22 min to 30 min in three cases studied.


Strain MC29 avian leukosis virus: immunologic relationships to other avian tumor viruses.


Reduction of rapid-eye-movement sleep after electroconvulsions--an experiment in cats on the mode of action of electroconvulsive treatment.


Enhancement of infectivity of murine cytomegalovirus in vitro by centrifugal inoculation. Centrifugation of murine cytomegalovirus inocula from a variety of sources onto secondary mouse embryo cell monolayers at 1,900 x g for 30 min regularly revealed 10- to 100-fold more infectious virus than could be found in the same materials using standard inoculation methods. Virus demonstrable only by centrifugation was present throughout the entire growth cycle in a constant proportion to virus measured without centrifugation. Extracellular growth curves of both populations revealed an 18- to 21-hr latent period, followed by a long-linear increase over the next 12 hr; final yield was 30 plaque-forming units (PFU) per cell. Centrifugation of cells prior to inoculation or after standard adsorption and removal of inoculum failed to result in any significant change in measured virus titer. However, even after 4-hr adsorption, the supernatant inoculum could be transferred and centrifuged onto a fresh monolayer resulting in the same increment of measurable virus. Neutralizing antibody and interferon were equally efficacious against 100 PFU of virus as defined by either method. Thus, this newly identified population of cytomegalo-virus represents the vast majority of potentially infectious units and appears to differ solely in ease of adsorption onto cell monolayers.


Replication of mengovirus in HeLa cells preinfected with nonreplicating poliovirus. The replication of mengovirus in HeLa cells preinfected with poliovirus in the presence of 10(-3) M guanidine was investigated. Although host cell protein synthesis is inhibited by the presence of nonreplicating poliovirus, it is found that mengovirus ribonucleic acid (RNA) and protein synthesis proceed normally under the same conditions. Furthermore, no effects on mengovirus growth by poliovirus can be detected either when Mengo protein synthesis is interrupted by Acti-Dione or when its RNA synthesis is reduced by incubation at 28 C. It is suggested that the poliovirus inhibitory factor may be able to distinguish between an RNA element required in the protein-synthesizing apparatus of the host cell and a comparable element in that of the heterologous virus.


Chromosomal aberrations and cloning efficiency in adenovirus type 12-infected hamster cells. The fate of hamster cells, abortively infected with adenovirus type 12, has been studied by correlation of chromosomal aberrations with induction of T antigens and cloning efficiency. The incidence of chromosomal changes paralleled to some extent the T antigen formation, but was inversely related to the cloning efficiency of the cells. At an input multiplicity of 100, within 24 hr after infection, nearly all of the cells or metaphases revealed the presence of T antigens and chromosomal lesions, respectively, but no clones of cells were obtained. Inhibition of cellular deoxyribonucleic acid synthesis was not noted during this period. Increasing doses of ultraviolet irradiation reduced, successively, the capacity of the virus to induce chromosomal aberrations and correspondingly improved cloning efficiency of the exposed cells. It is concluded that most, if not all, cells revealing chromosomal lesions 24 hr after infection fail to enter further mitoses.


Antiviral activity of polyacrylic and polymethacrylic acids. I. Mode of action in vitro. Polyacrylic acid (PAA) and polymethacrylic acid (PMAA) were investigated for their antiviral properties in tissue culture. Compared to other related polyanions, as dextran sulfate, polystyrene sulfonate, polyvinyl sulfate, and polyphloroglucinol phosphate, PAA and PMAA were found to be significantly more antivirally active and less cytotoxic. PMAA added 24 hr prior to virus inoculation inhibited viral growth most efficiently but it was still effective when added 3 hr after infection. Neither a direct irreversible action on the virus nor inhibition of virus penetration into the cell could explain the antiviral activity of PMAA. PMAA inhibited the adsorption of the virus to the host cell and suppressed the one-cycle viral synthesis in tissue cultures inoculated with infectious RNA.


Use of zonal ultracentrifuge systems for biophysical studies of rhinoviruses. This paper reports the use of zonal ultracentrifuge techniques to conduct biophysical studies of rhinoviruses grown with WI-38 cells. Good clean-out of infectivity from rhinovirus harvests was obtained with the continuous-flow B-V and B-IX rotors. Use of the B-V rotor resulted in the successful concentration of rhinovirus infectivity and antigenicity. Additional purification was achieved by the combined use of continuous-flow centrifugation and isopycnic banding procedures. Two particle sizes were found to be associated with the virus-infected cell harvests. The infectious 22-nm particle banded in density ranges of 1.38 to 1.40 g/cm(3) in CsCl and 1.26 to 1.27 g/cm(3) in potassium citrate. The 8.0 nm capsomere was composed of 2.0 nm subunits and banded with a density of protein at 1.28 g/cm(3) in CsCl. Equivalent sedimentation coefficients of 155 or 185, depending on particle density in sucrose, were calculated from rate zonal experiments by use of the B-IV zonal rotor.


Persistence of the viral genome in adenovirus type 12-infected hamster cells. Induction of T antigen by adenovirus type 12 was studied in growing and growth-inhibited cultures of the Nil-2 line of Syrian hamster cells. At a viral input multiplicity of 10, neoantigen was present in 100% of the cells by 24 hr. T antigen gradually disappeared in descendants of these cells so that 2 weeks after infection only 1% gave specific immunofluorescence. When cellular replication was prevented by addition of fluorodeoxyuridine, T antigen persisted in all cells for the 2-week period. Upon infection of growing cultures with purified (3)H-labeled adenovirus type 12 and autoradiographic analysis of the cells at various times thereafter, a gradual reduction in labeled nuclear loci was noted which paralleled the decrease in T antigen-containing cells. In nongrowing cultures, no change in labeled loci was noted. Correlation of T antigen and labeled loci revealed that fluorescent cells contained, on the average, about 10 times more silver grains than nonfluorescent cells. All of 92 preselected fluorescent cells showed labeled loci, whereas, of 100 nonfluorescent cells, 18 were free of silver grains. The implications of these findings are discussed.


Stabilization of enveloped viruses by dimethyl sulfoxide.


Early step in Sendai virus-mediated rescue of Rous sarcoma virus from virogenic mammalian cells.


Spin-labelled haemoglobin and the haem-haem interaction.


Recent developments in pathophysiologic studies of the renin-angiotensin system.


Hypertension and unilateral hydronephrosis.


Importance of the bursa of fabricius in resistance to disease. 1. Resistance to certain virus diseases.


Zoonoses in Britain: some present and potential hazards to man. Primate diseases.


Arteriography in the diagnosis of liver tumours.


Fibroblastoid transformation of malignant myeloblasts in vitro.


Inositol phosphates obtained from a phosphatido-peptide fraction of pig aorta.


Studies on polypeptides. XLI. The synthesis of [5-valine, 6-beta-(pyrazolyl-3)-alanine]-angiotensin II, a potent hypertensive peptide.


Serological diagnosis of amoebiasis by immunofluorescence. A positive serological diagnosis of amoebiasis could be made by immunofluorescence in 66 of 78 established cases, taking a serum titre of 16 or higher as diagnostic: at this level there were no false positives among 94 control sera. The test is simple and may be carried out on amoebic smears stored for several months in 2-octanol. The serological activity is largely confined to the IgG immunoglobulin fraction and is specific for Entamoeba histolytica; cross reactions were not detected with other protozoa. Gel diffusion serological analysis permitted a positive diagnosis of amoebiasis in 60 of the 78 cases, and, combining this with the immunofluorescence test, raised the diagnostic score to 71 cases.


Effects of testosterone on adenosine triphosphate and nicotinamide adenine dinucleotide levels, and on nicotinamide mononucleotide adenylytransfersee activity, in the ventral prostate of castrated rats.


The relationship between the pH-induced spectral change in ferriprotoheme and the substrate-induced spectral change of the hepatic microsomal mixed-function oxidase.


Effects of transplanted pituitary glands from non-arteriosclerotic virgin and arteriosclerotic breeder donors to hypophysectomized rats.


Structure-activity relationships of the adrenocorticotropins and melanotropins: the synthetic approach.


Aerosol bronchography. A preliminary report.


The variable fate of oily contrast media after lymphography compared with residues in other sites.


Hepatic oil embolization following lymphangiography in a child with neuroblastoma.


Roentgenographic abnormalities in phenotypic females with gonadal dysgenesis. A comparison of chromatin positive patients and chromatin negative patients.


Antibodies to enzymatic breakdown products of yeast tRNA.


Dwarfism, oligophrenia and degeneration of the elastic tissue in skin and cornea. A new syndrome?


Comparative studies on K -p-nitrophenylphosphatase, K -acylphosphatase and (Na + K)adenosinetriphosphatase in synaptosomes of rat brain.


The delayed effect of ACTH administration on the plasma corticoid level of normal elderly persons and patients with chronic brain syndrome.


Infectious bovine rhinotracheitis: clinical review, immunity, and control.


Bovine myxovirus parainfluenza-3 and its role in bovine respiratory disease.


Lipid composition of chloroplasts isolated by aqueous and nonaqueous techniques. Chloroplasts isolated from tobacco leaves in 0.5 M sucrose solution (the 1000 g pellet) contained 83% of the total cellular monogalactosyl diglyceride, 88% of the digalactosyl diglyceride, 76% of the sulfolipid, and 74% of the phosphatidyl glycerol. Phosphatidyl inositol was concentrated in the 15,000 g pellet. Phosphatidyl choline and phosphatidyl ethanolamine were concentrated in the 15,000 g supernatant fraction. Chloroplasts isolated from tobacco leaves by a nonaqueous technique in hexane-carbon tetrachloride show a glycerolipid composition similar to that found in chloroplasts isolated in the aqueous system, even though some lipid, particularly monogalactosyl diglyceride, is extracted by the organic solvent during the process.


Thin-layer chromatography of the phosphoinositides. Thin-layer chromatography for the separation of mono-, di-, and triphosphoinositides (0.3-3 micro g total phosphorus) is described.


In vivo and in vitro studies of gluconeogenesis in meal-fed and nibbling rats.


Effect of diet, dietary regimens and strain differences on some enzyme activities in rat tissues.


Serotonin infusion in rats with experimental renovascular hypertension.


Toxicity of emetine to isolated embryonic chick-heart cells.


Mechanism of flow improvement by the addition of fine particles to bulk solids.


Reversion of properties in cells transformed by polyoma virus.


Erythrocyte ATP: D-fructose-6-phosphate 1-phosphotransferase (phosphofructokinase) activity in children with normałG trisomic mosaic Down's syndrome and in normal and Down's syndrome controls.


Electrodermal levels and fluctuations during normal sleep.


Carbohydrate metabolism in cancer cells and the molecular correlation concept.


Treatment of American cutaneous leishmaniasis with cycloguanil pamoate.


Slipped upper femoral epiphysis. Clinical study on aetiology.


Respiratory infections in the American tropics.


Immunodiffusion of bluetongue virus.

Immunodiffusion of bluetongue virus.


Cholelithiasis in rabbits: effects of bile constituents and hormones on dissolution of gallstones.


Aberrant characteristics and virus-like particles of bovine mammary cell cultures.


Modified biochemical tests for characterization of L-phase variants of bacteria. Biochemical capabilities of bacterial and L-phase organisms of 15 bacterial strains were examined by using a variety of modified routine diagnostic biochemical tests. The results demonstrated that 13 of the tests examined were suitable for use with L-phase variants, that L-phase variants and revertant bacterial phase organisms retained diagnostically significant capabilities of the respective bacterial or L-phase organisms from which they were derived, and that the 13 biochemical tests could be usefully employed to relate a given L-phase variant to a given bacterial phase organism, to distinguish L-phase variants of different species, and to aid in the identification of nonreverting L-phase variants.


Localization of lecithinase activity in Clostridium perfringens.


Photodynamic antiviral substance extracted from chlorella cells.


Survival of a picornavirus and its infectious ribonucleic acid after aerosolization.


Viral depuration by assaying individual shellfish. A study was carried out to further evaluate the practicability of viral depuration by assaying individual shellfish. The Northern quahaug and a strain of the type 1 attenuated poliovirus were used as the working model. Two types of depuration systems were employed: the small experimental tanks and a pilot-size tank with a capacity of approximately 24 bushels (836 liters) of shellfish. Volumes of the individual shellfish samples were found uniform throughout the experiments when a prior selection for the weight of the shellfish was made. There was also no significant difference in volumes of the individual samples during the course of depuration (24 to 96 hr). Under controlled hydrographic conditions, however, the uptake of virus in individual shellfish varied considerably. In general, the individual variability reached 10- to 100-fold. This wide variation would explain the variability of viral contents obtained in pooled samples during depuration as reported previously. During a later phase of depuration, although a great majority of shellfish were free of the virus, a few still harbored minimal amounts of contaminants. The presence of virus in some of the shellfish after various periods of depuration would, theoretically, be obscured by the pooling of the sampled shellfish. Further examination of the negative samples by assaying larger quantities than those routinely used revealed that a few still contained virus. To simulate naturally polluted shellfish as closely as technically possible, shellfish were polluted with minimal amounts of virus. The shellfish were cleansed more rapidly by the depuration process than were those polluted with more virus. Since the naturally polluted shellfish were shown to contain less virus than those studied in the laboratory, it is anticipated that the former type of shellfish may be cleansed more readily by this process within a reasonable period of time. Justification for a field trial of depuration in this country is presented.


Functional significance of the tubular agranular endoplasmic reticulum in the adrenocortical cells of albino rats.


Effects of dibutyryl cyclic AMP on adrenal growth and steroidogenic capacity.


Stimulation of progesterone release and estrous behavior by ACTH in ovariectomized rodents.


Steroid hormone transformations by endocrine organs from pregnant mammals. 3. Biosynthesis and metabolism of progesterone by the Mare placenta in vitro.


Induction of hepatoma and systemic amyloidosis in mice by 4-(dimethylamino)azobenzene feeding.


The characterization and thin-layer chromatographic quantitation of the human metabolite of 7-deoxy-7(S)chlorolincomycin (U-21,251 F).


Comparative intermediary metabolism of vegetative cells and microcysts of Myxococcus xanthus. Crude extracts of both vegetative cells and glycerol-induced microcysts of Myxococcus xanthus contained the following enzyme activities: phosphofructokinase, phosphoglucoisomerase, fructose-1,6-diphosphatase, fructosediphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase, phosphopyruvate carboxylase, citrate synthase, isocitrate dehydrogenase, alpha-ketoglutarate dehydrogenase, succinate dehydrogenase, malate dehydrogenase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphoglucomutase, and uridine diphosphate glucose pyrophosphorylase. With the exception of isocitrate dehydrogenase, which was present at a fivefold higher concentration in microcysts, all activities in extracts from both types of cells were essentially equal. Hexokinase and pyruvate kinase could not be detected in extracts from either type of cell. Microcysts metabolized acetate at a lower rate than did vegetative cells. Most of this decrease was reflected in a substantial decrease in ability of microcysts to oxidize acetate to CO(2). In addition, microcysts and vegetative cells showed a different distribution of (14)C-label from incorporated acetate.


Lymphocytosis and histamine sensitization of mice by fractions from Bordetella pertussis. Administration of Bordetella pertussis cell extracts induced in mice hypersensitivity to histamine, as well as pronounced leukocytosis and hypoglycemia. The leukocytosis was mainly caused by an increase in the small lymphocytes in the circulating blood, and it was most pronounced 3 to 4 days after injection of B. pertussis extracts. Rabbit antimouse lymphocyte serum produced a decrease in the lymphocyte count in normal mice, as well as in mice treated with B. pertussis extracts. This depression in lymphocytes was observed whether the antilymphocyte serum was given 1 day or 2 days after the administration of B. pertussis extracts. The increased histamine sensitivity and hypoglycemia of mice treated with B. pertussis extract were not affected by treatment with antilymphocyte serum, although a marked lymphopenia was present. These observations indicate that the three phenomena observed in pertussis-treated mice are independent of each other.


Respiratory components of Aspergillus niger mitochondria. The respiratory components of tightly coupled mitochondria from the filamentous fungus Aspergillus niger were studied. Cytochromes a + a(3), b, and c + c(1) were identified by difference spectra. The cytochrome spectra were qualitatively similar to yeast and rat liver mitochondria. The mitochondria contained, per gram of protein, an average of 2.9 and 7.0 mumoles of ubiquinone and nicotinamide adenine dinucleotide, respectively.


Molar growth yields as evidence for oxidative phosphorylation in Streptococcus faecalis strain 10Cl. During the aerobic growth of Streptococcus faecalis strain 10C1, with limiting levels of glucose as the substrate, a molar growth yield (Y) of 58.2 g (dry weight) per mole of glucose was obtained. Under these conditions of growth, glucose was dissimilated primarily to acetate and CO(2). The incorporation of (14)C-glucose into cell material was no greater under aerobic conditions than during anaerobic growth. Assuming an adenosine triphosphate coefficient of 10.5, the aerobic Y cannot be explained solely on the basis of substrate phosphorylation and would appear to substantiate previous enzymatic evidence for oxidative phosphorylation in this cytochromeless species. With mannitol as the substrate, an aerobic Y of 64.6 was obtained. Extracts of mannitol-grown cells contained a nicotinamide adenine dinucleotide (NAD)-linked mannitol-1-phosphate (M-1-P) dehydrogenase. The difference in aerobic Y values with mannitol and glucose as substrates would indicate that the in vivo P/O ratio from the oxidation of reduced NAD generated by the oxidation of M-1-P approximates 0.6. The Y values with pyruvate and glycerol as substrates under aerobic conditions were 15.5 and 24.7, respectively.


Cytology of spore formation in Clostridium perfringens. The sequential morphological events in spore formation by Clostridium perfringens type D were observed in Ellner's medium where 80 to 100% of the cells formed spores. Gross structural changes were studied with the light microscope under phase-contrast, and in fixed cells by the use of both nigrosin and Giemsa preparations. Fine structure was examined with the electron microscope in both thin sections and frozen-etched preparations. During the first 3 hr of incubation, the original rod-shaped cells became ellipsoid to ovoid in shape; by 5 to 6 hr, subterminal spores had developed within these enlarged cells. The fine structural sequence was in most respects identical to that in other Bacillaceae, although some stages were illustrated with particular clarity. A unique feature was the development of a convoluted, membranous exosporium which adhered to the outer surface of the two coats and had an unusual fine structure resembling a rectangular array of subunits.


Comments on the action of quaternary ammonium compounds at the receptor site.


Distribution and fatty acid composition of phosphoglycerides in normal human brain. A thin-layer chromatographic procedure for the isolation of tissue phospholipids and their subsequent analysis is described. The method has been applied to the determination of the fatty acids of phosphoglycerides in human brain from the early fetal stage to old age. The study shows changes in the distribution and fatty acid composition of each phosphoglyceride in normal brain, although they are quite small after early childhood. A lipid-specific fatty acid pattern for each of the four major phosphoglycerides was found. Besides this, the pronounced differences between fatty acids of the lipids from the cerebral cortex and from the adjacent white matter justify speaking of a tissue-specific fatty acid pattern for brain phosphoglycerides. The phospholipids of cerebral white matter contained more monoenoic acid but much less polyunsaturated fatty acid than those of cerebral cortex. The brain phosphoglycerides also showed an age-dependent fatty acid pattern. With increasing age the concentration of the fatty acids of the linoleate family diminished while that of the linolenate family increased. Brain inositol phosphoglycerides, the fatty acid composition of which has not been studied systematically before, were characterized by a large concentration of arachidonate which was nearly as high for white as for gray matter and showed only small changes with age.


Analysis of simian virus 40-induced transformation of hamster kidney tissue in vitro. V. Variability of virus recovery from cell clones inducible with mitomycin C and cell fusion. Clones of simian virus 40-transformed hamster kidney cells from which infectious virus could be recovered and clones which did not yield virus by the overlay technique were subjected to chemical (mitomycin C) induction and to co-cultivation and fusion studies with indicator cells. The results of these studies indicate that the complete viral genome is present in all clones tested but that considerable heterogeneity with respect to inducibility exists among the clones. It is suggested that differences either in the number of viral genomes per transformed cell or in the status of the viral genome in transformed cells exist among the various clones. Furthermore, the inducibility of the clones may be correlated with their malignant potential.


Physicochemical characteristics of rubella antigens theta and iota.


Individual variations in rate of skeletal maturation.


Identification of plasma angiotensinase as aminopeptidase.


Failure to induce scurvy by ascorbic acid depletion in a patient with Hurler's syndrome.


Thermography as an investigative method.


Breakdown in maternal protection: infections.


Synthetic beta 1-24-corticotropin stimulation of cortisol biosynthesis by rabbit adrenal tissue.


Isolation of crystalline estriol-3-glucuronide from human pregnancy urine.


Cylindroma involving the paranasal sinuses.


Vitamin K1-3H in man. Its intestinal absorption and transport in the thoracic duct lymph.


Analysis of factors affecting lactic dehydrogenase subunit composition determinations.


An analog model for acetylcholine release by motor nerve endings.


Methodology and techniques in visual field studies.


The effect of ochratoxin A on glycogen storage in the rat liver.


Ophthalmological aspects of neuroblastomatosis: a study of 53 verified cases.


Systematic radiologic approach for the diagnosis of temporal bone disease.


The topographic and subcellular distribution of some cation-activated phosphohydrolases in rat brain.


The cholinergic system in insect development.


Selection of therapy of glomus jugulare tumors.


Glomus jugulare tumors. Long-term tumors.


Jugular venography.

Jugular venography.


Neuro-otologic tests and examination.

Neuro-otologic tests and examination.


Radiologic diagnosis of neuro-otological problems by conventional radiology.


Action of various sympathomimetic amines on the isolated stripped vas deferens of the guinea-pig. 1. The contractile potencies of some sympathomimetic amines and cholinergic drugs were studied in the isolated, stripped vas deferens of the guinea-pig.2. alpha-Methylnoradrenaline, adrenaline and metaraminol produced the same relative maximal responses as noradrenaline, which served as reference drug; on the other hand, octopamine, dopamine and alpha-methyldopamine were much less active. Short term pretreatment of the animals with reserpine did not affect the maximal relative responses.3. Tyramine, alpha-methyl-m-tyramine and hordenine methiodide had little effect on the untreated preparation. In the presence of just threshold concentrations of noradrenaline, the relative maximal responses to tyramine and hordenine methiodide were markedly increased.4. The relative maximal response to carbachol was the same as that to noradrenaline, while that to DMPP was smaller. In contrast to carbachol, the action of DMPP was potentiated by pretreatment of the preparation with noradrenaline.


The interaction between angiotensin and sympathetic vasoconstriction in the isolated artery of the rabbit ear. 1. Infusions or injections of angiotensin into the isolated ear artery preparation of the rabbit produced only feeble vasoconstrictions and depressed the sensitivity of the preparation to sympathetic stimulation, tyramine and noradrenaline.2. The anti-sympathetic effect of angiotensin was not prevented or reversed by noradrenaline or cocaine.3. The vasoconstrictor response to angiotensin was usually markedly increased during sympathetic stimulation or other procedures which liberate endogenous noradrenaline (for example, infusion of tyramine), but not when the perfusion pressure was raised by increasing the flow rate or by infusing noradrenaline.4. The vasoconstrictor action of tyramine was also potentiated by sympathetic stimulation.5. The potentiation by sympathetic stimulation of the responses to angiotensin was prevented by reserpine, guanethidine and phentolamine in concentrations which abolished sympathetic vasoconstriction.6. It is suggested that angiotensin facilitates the release of endogenous noradrenaline and owes part of its vasoconstrictor activity to this property.


The mobilization of free fatty acids from rabbit adipose tissue in situ. 1. The epigastric adipose tissue of rabbits has been prepared so that the effects of close arterial injections and infusions on blood flow and release of free fatty acids (FFA) can be studied. The effects of pharmacologically active agents and hormone preparations have been investigated.2. Release of FFA was stimulated by synthetic adrenocorticotrophic hormone (ACTH), alpha and beta melanophore stimulating hormone (MSH), porcine growth hormone, glucagon, thyrotropic hormone (TSH) and luteotropic hormone (LTH). Single injections of fat-mobilizing agents produce a sustained rise in the release of FFA.3. Although pitressin caused release of FFA, synthetic vasopressin and oxytocin failed to do so. The FFA releasing activity of pitressin has therefore been attributed to a contaminant.4. Catecholamines were found not to stimulate release of FFA from this fat depot, but were found to increase plasma FFA when infused intravenously.5. Injections of acetylcholine, histamine, bradykinin, 5-hydroxytryptamine, synthetic arginine vasopressin, and lysine vasopressin, oxytocin, angiotensin and FSH did not stimulate release of FFA although marked effects on blood flow were produced.6. Injections of prostaglandin E(1) gave sustained increases in blood flow, and inhibited FFA release when stimulated by growth hormone.7. The mobilization of FFA is sometimes associated with an increased rate of blood flow.


Non-specific inhibitory effects of morphine-like drugs on transmission in the superior cervical ganglion and guinea-pig isolated ileum.


Effect of rabbit antibodies against angiotensin-II on the pressor response to angiotensin-II and renal hypertension in the rat. 1. In the intact or nephrectomized rat, the pressor responses to synthetic angiotensin-II-amide, beta-angiotensin-II and native "rat angiotensin" could be inhibited by intravenous injection of rabbit serum containing antibodies against synthetic angiotensin-II-amide.2. The pressor responses to vasopressin or noradrenaline were not influenced by anti-angiotensin serum.3. A single injection of anti-angiotensin serum inhibited the pressor responses to angiotensin-II-amide for a period of 2.5 hr.4. Intravenous infusion or daily intraperitoneal injection of anti-angiotensin serum did not influence blood pressure levels in renal hypertensive rats.


Neurophysiological methods in anaesthesia research.


Anaesthetic action of magnesium ions.

Anaesthetic action of magnesium ions.


Nuclear magnetic resonance studies of junctional transmitter-receptor interactions.


Suppression studies in affective disorders.


Enzymic flux rates in vivo through the Embden-Meyerhof pathway and the nucleotides of the mouse liver.


Oxygen consumption in adult Echinostoma revolutum.


Carbohydrate utilization by Entamoeba histolytica.


Histochemical changes in rat adrenal cortex influenced by thioacetamide and ACTH.


Mode of action of rat liver phosphodiesterase on a polymer of phosphoribosyl adenosine monophosphate and related compounds.


Biosynthesis of branched chain deoxysugars. IV. Isolation of cytidine diphosphate 6-deoxy-3-C-methyl-2-O-methyl-4-O-(O-methyl-glycolyl)-L-aldohexopyranoside from Azotobacter vinelandii.


Deacylated transfer ribonucleic acid as a factor for peptide chain initiation in rabbit reticulocyte systems.


Glutamine phosphoribosylpyrophosphate amidotransferase. Purification, substructure, amino acid composition, and absorption spectra.


The accumulation of glucose 6-phosphate from glucose and its effect in an Escherichia coli mutant lacking phosphoglucose isomerase and glucose 6-phosphate dehydrogenase.


Studies in fluorescence histochemistry. V. The influence of trace metals on the fluorescence of periodate-oxidized mucosubstance salicylhydrazones.


Synthesis of functional proteins in x-irradiated mammalian cells.


Role of the vagus nerve in the control of the concentration of angiotensin II in the circulation.


Behaviour of infectious RNA from four different viruses in continuously subcultured Aedes aegypti mosquito embryo cells.


Viruses isolated from sheep.

Viruses isolated from sheep.


Skeletal renewal and metabolic bone disease.


Mixed mesodermal tumour of the uterus occurring after radiotherapy.


Dysmorphogenesis: the role of infection.


Studies of wheat-embryo ribosomal RNA. I.


Effect of gamma-radiation on aqueous solutions of DNA's of different base composition.


Enhancement by 2,4-dichlorophenoxyacetic acid of chromatin RNA polymerase in soybean hypocotyl tissue.


Increased cardiovascular reactivity caused by depletion of endogenous renin in dogs.


Conflicting hypotheses on experimental silicotic fibrogenesis: new experimental data.


Role of connective tissues in large pulmonary airways.


Some features of the central co-ordination of a fast movement in the crayfish.


Amechanical model of the mammalian muscle spindle.


Mode of action of insulin in the regulation of protein biosynthesis in muscle.


The actions of insulin and catabolic hormones on the plasma membrane of the fat cells.


Neural control of anterior pituitary functions.


Endocrine factors in the pathogenesis of plasma cell tumors.


Effects of calcium and magnesium on sympathetic transmission in human fallopian tube.


Lysolecithin inhibition of mitochondrial metabolism.


Respiratory control in submitochondrial particles obtained by sonication.


The intramembrane location of alcohol anesthetics.


Preservation of glucose 6-phosphatase activity in formaldehyde-fixed fresh frozen sections.


Cytomegalovirus infection complicating open-heart surgery.


Mixed haemadsorption with Herpes simplex virus.


Cystosarcoma phyllodes of the breast. A statisical study of forty cases.


The effect of bone marrow transplantation on the survival of patients with advanced cancer.


The production and effect of interferon in organ cultures of calf trachea.


Potentiation by silica of the growth of Mycobacterium tuberculosis in macrophage cultures.


Enzymatic analysis of sclera.

Enzymatic analysis of sclera.


Quantitative histochemistry of histamine, and histidine decarboxylase in the stomach of rat, pig, and dog in various functional states.


Epidemiology of pancreatic cancer in Connecticut.


Radicals formed by electron transfer to pyrimidine derivatives.


Radical formation in cysteamine free base and cystamine.2HCl by reaction with atomic hydrogen and by gamma-irradiation.


Effect of different proteins (casein, gluten, zein) and supplements of amino acids (lysine, tryptophan) on hepatic injuries induced by p-dimethylaminoazobenzene.


The syndrome of congenital adrenocortical unresponsiveness to ACTH. Report of six cases.


Prevention of the normal postnatal increase in drug-metabolizing enzyme activity in rat liver by a pituitary tumor.


Cander risk in intraductal papilloma and papillomatosis.


Renal function in the iso- lated perfused kidney.


The control of corpus luteum function in the ferret.


Renal stone.

Renal stone.


Meningiomas and fibroblastic neoplasia in calves induced with the bovine papilloma virus.


Nonvirus-associated antigens in virus-induced mouse mammary tumors.


The heterogeneity of DNA polymerases in rat liver and hepatomas.


The antigenicity and immunogenicity of cell-free extracts of chemically induced murine sarcomas.


S-adenosylmethionine synthetase activity in some normal rat tissues and transplantable hepatomas.


Antigenic characteristics of lymphomas induced by radiation leukemia virus (RadLV) in mice and rats.


Comparative studies of fluorinated pyrimidines with various cell lines.


Metabolic properties of mouse transplantable adenocarcinoma.


Angiotensin and the Stouffer prize.

Angiotensin and the Stouffer prize.


Diminished baroreflex sensitivity in high blood pressure.


Oral medicine seminar. No. 16--oral cancer.


Teratological studies with sulphonamides and their implications.


The effect of rubella and herpesvirus homonis on the pre- and post-implantation stages of pregnancy in laboratory animals.


Enzyme ontogeny in normal and hemimelic limbs of mice.


Hepatorenal glycogenosis (type I glycogenosis) and carcinoma of the liver.


Norethandrolone in cystic fibrosis of the pancreas.


Reserpine- and chlorpromazine-induced changes in hypothalamo-hypophyseal-adrenal system in rats in the presence and absence of hypothermia.


Some features of barbiturate interaction and inhibition of NADH-cytochrome C oxidoreductase in respiring systems.


The direct effect of norepinephrine of angiotensin on the pulmonary veins of intact dogs.


Nursing rediscovered.

Nursing rediscovered.


Foreword and general management.

Foreword and general management.


Treatment of metabolic neuropathies.

Treatment of metabolic neuropathies.


Treatment of deficiency and alcoholic neuropathies.


Treatment of toxic and drug induced neuropathies.


Treatment of neuropathies accompanying inflammatory, vascular and neoplastic conditions.


Avian virus-induced tumors.

Avian virus-induced tumors.


"Asterinsäure"--an acetylenic carotenoid.


Tissue culture study of glioblastoma cells with addition of autologous lymphocytes.


Hepatic lobectomy for hepatoblastoma in infants and children.


Cholinergic link hypothesis and sympathetic transmission at the nictitating membrane of the dog.


Reduction and detection of aromatic nitro-compounds on thin layers of silica gel.


Improved thin-layer chromatographic separation of amanita toxins on Silica Gel G chromatoplates.


Variations in the cell cycle during azo dye hepatocarcinogenesis.


Enhancement of urethan induction of hepatomas in mice by prior partial hepatectomy.


Observations on a cell line producing mammary tumor virus.


Methods for detection of antibody to the mammary tumor virus.


Site of genetic regulation on hypophysial graft-induced mammary gland development.


Establishment of a cell line from an inbred mouse strain for viral transformation studies: simian virus 40 transformation and tumor production.


Quantitative distribution of virions in virus-induced murine leukemia.


Preliminary note. Identity of a herpesvirus antigen from Burkitt lymphoma of man and the Lucké adenocarcinoma of frogs.


Current trends in nursing education with implications for nursing service.


In vivo effects of anti-angiotensin II on the renin-system. Share of the reninsystem in the normal blood pressure level, and the mechanism of the changed response to renin in nephrectomised rats.


Virus-like particles in Rous rat sarcoma.


Investigations on the enzymes and toxins of staphylococci. Relations of pH to growth and production of enzymes.


Zoster encephalitis: a case report.

Zoster encephalitis: a case report.


The effect of hypnotics on rapid eye movement (REM).


Histochemistry of human cervical posterior root ganglion cells and a comparison with anterior horn cells.


Histidine regulatory mutants in Salmonella typhimurium. V. Two new classes histidine regulatory mutants.


Histidine regulatory mutants in Salmonella typhiumium. VI. Dominance studies.


Primary mucoid adenocarcinoma of the prostate gland with bladder invasion.


The effect of the seed of Musa superba Roxb. on small-pox virus.


Ultrastructural observations on the droplets of experimentally-induced goitres in Xenopus laevis with especial reference to the development of Uhlenhuth colloid cells.


Central actions of lidocaine--synaptic transmission.


Methotrexate therapy in trophoblastic disease at the Provinical Taipei Hospital (1955-1966). A re-evaluation and proposals.


Action of lidocaine in the central nervous system.


Local anesthetic seizures.

Local anesthetic seizures.


The electromechanical response to stimulation by the Block-Aid Monitor.


Influence of microorganisms on mammalian metabolism and nutrition, with specific reference to oxygen, consumption, carbon dioxide production, and colonic temperature.


Effect of gold salts and other drugs on the release and activity of lysosomal hydrolases.


The assay of adenosine 3',5'-cyclic monophosphate and guanosine 3',5'-cyclic monophosphate in biological materials by enzymatic radioisotopic displacement.


The theoretical basis for the measurement.


Investigations of inter- and intramolecular interations in flavin--adenine dinucleotide by proton magnetic resonance.


Studies on oxidative phosphorylation. XVI. Sulfhydryl involvement in the energy-transfer pathway.


Bronchopneumonia with serious sequelae in children with evidence of adenovirus type 21 infection. Twenty-five cases of severe pneumonitis occurring among children aged 3 to 18 months were observed in Auckland, New Zealand, in 1965. An apparent relationship was established between a uniform disease picture and recovery of adenovirus type 21. Two died, and of the survivors 60% had permanent lung damage, probably resulting from obliterative bronchiolitis. Twenty per cent. were left with proved bronchiectasis. The possible relationships of adenovirus pneumonia to malnutrition or a genetic factor on the one hand and to the development of bronchiectasis on the other are discussed.


Radioactive selenium labeling of rat insulin in vivo.


Motor nerve terminals as the site of initial functional changes after denervation. For the cat soleus nerve-muscle system, motor nerve section 48 hr prior to in situ experiment causes certain characteristic transmission losses. Responses to repetitive stimulation are sharply altered: The capacity to transmit iterative stimulation is severely reduced; post-tetanic potentiation and the post-tetanic repetition of soleus nerve terminals responsible for it are also greatly impaired; a phenomenon of post-tetanic depression was frequently observed. However, function of the extramuscular axons appears normal and single impulse transmission is usually not seriously affected. The loss of reactivity to repetitive stimulation has been traced to soleus motor nerve terminals. In view of these data and the known absence of denervation hypersensitivity at this time, the earliest functional failure may be said to occur in the unmyelinated terminals. This subacutely denervated preparation therefore offers a simple means of evaluating motor nerve terminal responsiveness.


Transcription during bacteriophage T4 development: requirements for late messenger synthesis.


Morphogenesis of poliovirus. I. Association of the viral RNA with coat protein.


Embryotoxic activity of hypoglycin-A in the rat.


Morphological changes in the rat hypothalamus and adrenal cortex in the early postnatal period after ACTH and hydrocortisone administration, stress, and adrenalectomy.


Vasopressin test in central nervous system-pituitary disorders.


On the sulfur components of iron-sulfur proteins. I. The number of acid-labile sulfur groups sharing an unpaired electron with iron.


Early release of growth inhibition in cells infected with Rous sarcoma virus.


Regulation of transcription of the SV40 DNA in productively infected and in transformed cells.


Inhibition by nucleotides of liver microsomal glucose-6-phosphatase.


Flavoproteins of the electron transport system and the site of action of amytal, rotenone, and piericidin A.


The effects of different preparative approaches on the ultramorphological appearance of the Kirkman-Robbins hepatoma in the hamster.


Malignant neoplasms of the central nervous system, Onatrio Cancer Foundation clinics, 1950-1964.


Neurogenic control of the blood pressure in hypertension.


Modern cultism.

Modern cultism.


Intrabulbar inoculation of Japanese encephalitis virus to mice.


Cytochrome oxidase.

Cytochrome oxidase.


Hypotheses on physiological roles of prostaglandins. Prostaglandin, a smooth muscle-stimulating depressor acidic lipid discovered int he human seminal plasma in 1935, is now used as a generic term for a family of closely related derivatives of prostanoic acid which are widely distributed in animal tissues.  Prostaglandins are biosynthesized from arachidonic acid and dihomo-gamma-linolenic acid, both of which are derived from dietary linoleic acid.  This finding provided a link to the observation that linoleic acid is an essential constituent of the diet.  It is possible that prostaglandin compounds play a biochemical role fundamental to many, or all, animal cells.  They have been implicated in sperm transport, menstruation, parturition, and control of placental blood flow.  They may also play a role in the central nervous system, although this role may well be other than that of synaptic transmitter.  Release of prostaglandin from various tissues is brought about by nerve stimulation.  In adipose tissues, the amounts released may be sufficient to inhibit formation of cyclic AMP by the released noradrenaline, thus providing a local negative feedback mechanism.  Prostaglandins also possibly play a role in muscular contractility, essential fatty acid deficiency, inhibition of lipid mobilization, atherosclerosis, and thrombosis.


The two stages of slow wave sleep in the cat and their relation to REM sleep.


The effect of high doses of 5-hydroxytryptophan on a patient with trisomy 21. Clinical, chemical and EEG correlations.


Organismic supercategories. I. Proposals for a general unitary theory of systems.


Monosynaptic reflex during natural sleep in the kitten.


Phosphonolipids. XVII. Synthesis of ethylene glycolphosphonic acid analogues of lecithins.


Enhancement of radiation effects by phosphate buffer at 77 degrees K.


Mucus producing carcinoma of the kidney. Report of a case.


Adrenocorticotropins. XXXIX. The solid phase synthesis of methion-glutamyl-histidyl-phenylalanyl-arginyl-tryptophyl-glycine.


Behavior of equine infectious anemia virus in cell culture and development of a diagnostic test for the disease.


The fine structure of proliferating cells in preneoplastic rat livers during azo-dye carcinogenesis. The continuous feeding of the carcinogenic aminoazo dye DAB to rats produces hyperbasophilic foci in the preneoplastic livers. After injections of thymidine-(3)H into the rats, such foci were isolated from the livers and studied by radioautography with the phase-contrast and electron microscopes. In these foci, the only cells found to be proliferating, as determined by the uptake of thymidine-(3)H into their nuclei, were a poorly differentiated type; well differentiated hepatocytes in the same regions were not labeled with the isotope. The labeled cells had an irregular cell outline and a high nucleocytoplasmic ratio; the cytoplasm had almost completely lost the specialized elements characteristic of hepatocytes; the irregular nuclei with prominent nucleoli, the altered mitochondria, and the increased free ribosomes noted in these cells are features which are characteristic of neoplastic cells induced by DAB. Thus, it seems likely that the hyperbasophilic foci represent the sites of extensive dedifferentiation of hepatocytes followed by rapid cellular proliferation, leading to neoplastic growth.


Synergistic effect in viral-bacterial infection. I. Combined infection of the respiratory tract in mice with parainfluenza virus and Hemophilus influenza.


The ultrastructure of a tumor of the glomus jugulare.


Hyperbaric oxygen radiotherapy and the need for preliminary myringotomy.


Glomus tumor of the nasal cavity.

Glomus tumor of the nasal cavity.


Tumors of major salivary glands. Review of 100 consecutive cases treated at the Royal Victoria Hospital, Montreal, Canada.


Oncogenicity of mixtures of adeno-associated virus and adenovirus type 12.


Mössbauer effect in the ferredoxin of Euglena.


Biological significance of a peculiar pattern of hormone release from iodine deficient rat goitres.


Central pressor effect of angiotensin mediated by the parasympathetic nervous system.


Cellular and humoral immunity to different types of human neoplasms.


Mediastinal cysts and tumors.

Mediastinal cysts and tumors.


Antibody synthesis in vitro by lymph nodes from hamsters bearing adenovirus 12 tumours.


The in vivo effects of quartz and carbon dusts on the activity of cytochrome C oxidase and the DNA and RNA content of lung tissue.


Pediatric neurology.

Pediatric neurology.


Studies of the development of congenital anomalies in embryos of riboflavin-deficient, galactoflavin fed rats. II. Role of the terminal elec-tron transport systems.


Dialysis and transplantation: some interactions and comparisons.


Biosynthesis of rubber.

Biosynthesis of rubber.


Ketogenesis and its regulation.

Ketogenesis and its regulation.


The biochemistry of the functional psychoses.


Experiences with AGA thermovision in breast disease and survey programmes.