The mode of cerebello-thalamic relay transmission investigated with intracellular recording from cells of the ventrolateral nucleus of cat's thalamus.


Synaptic potentials recorded in brain slices and their modification by changes in the level of tissue ATP.


Urinary adenosine 3',5'-monophosphate excretion in affective disorders.


The influence of collengase on the periodontal ligament of orthodontically rotated teeth.


Preliminary results of azathioprine treatment in severe rheumatic disease.


Lymphangiography.

Lymphangiography.


Elasticity of human skin in vivo. A study of the physical properties of the skin in rheumatoid arthritis and the effect of corticosteroids.


Quantitative radiation effects in hemoproteins and their constituents. In order to verify the "radiation protection" effect of prosthetic groups in irradiated conjugated proteins and the influence of their electronic structure on intramolecular excitation transfer, two series of substances were examined. The first was composed of ferrihemoglobin and its constituents and the second was composed of similar hemoproteins with heme groups with major differences in electronic structure. The results point to a strong radiation protection effect of the heme groups and a dependence of radical yield of hemoproteins on the electronic structure of the heme. A possible interpretation of this latter effect is discussed.


Primary clostridial pneumonia and empyrema.


Evaluation of the calcifying characteristics of biological fluids and inhibitors of calcification.


Ultrastructural-characteristics of surface IgM reactive malignant lymphoid cells.


Sural nerve biopsies in pediatric neurological disorders.


Small angle light scattering of bioparticles. 2. Cells and cellular organelles.


Processing of 45 s nucleolar RNA.

Processing of 45 s nucleolar RNA.


Mixed tumour of sub-maxillary gland in a young boy. Report of a case.


Nerve growth factor in human sera.

Nerve growth factor in human sera.


The administration of the nerve growth factor to children with widespread neuroblastoma.


Congenital atypical benign chondroblastoma of a rib.


Pure gonadal dysgenesis with progressive hirsutism.


Postoperative neurological complications of antibiotic therapy.


Virus pneumonia in calves.

Virus pneumonia in calves.


Effect of diethylpyrocarbonate on the conformation and enzymic activity of d-glyceraldehyde-3-phosphate dehydrogenase.


Duality of conduction mechanism in bipolar cells of the frog retina.


Physiologic and pharmacologic regulation of bone resorption.


Information content of end-plate potentials (short communication).


Catecholamines and metabolism of human adipose tissue. I. Comparison between in vitro effects of noradrenaline, adrenaline and theophylline on lipolysis in omental adipose tissue.


Reaction mechanism of 20 beta-hydroxysteroid dehydrogenase determined by equilibrium rate exchange.


Studies on the respiratory chain-linked reduced nicotinamide adenine dinucleotide dehydrogenase. XVI. Characteristics of the membrane-bound dehydrogenase in Candida utilis and Saccharomyces cerevisiae.


Different molecular forms of potato apyrase.


Inhibition of respiration in submitochondrial particles by uncouplers of oxidative phosphorylation.


Comparative kinetic behavior of the photoinduced EPR signal observed in whole cells and cell free preparations of Rhodospirillum rubrum.


Long-term use of depot tetracosactrin.

Long-term use of depot tetracosactrin.


Correlation between angiographic diagnosis and histology of pancre atic insulomas.


Rate of disappearance of endogenous insulin from the circulation after removal of insulinomas. In two patients with insulinomas a rapid rate of disappearance of insulin from the circulation was found after the tumours had been removed. In a third case the rate of disappearance was slower but the insulin was not immunologically identical to normal human insulin standards, as it was in the other two cases.


Simultaneous determination of cortisol and corticosterone in urine.


The natural history of primary and secondary malignant tumours of the liver. 3. The prognosis for patients with hepatic metastases from pancreatic carcinoma verified by laparotomy.


Some studies on the effect of androgens on adrenal cortical function of rats.


Response of chick oviduct adenyl cyclase to steroid hormones.


Stimulation of corticotropin release by corticotropin-releasing factor (CRF) or by vasopressin following intrapituitary infusions in unanesthetized dogs: inhibition of the responses by dexamethasone.


Prevention of Mammary gland defects in experimental congenital adrenal hyperplasia due to inhibition to 3 beta-hydroxysteroid dehydrogenase in rats.


Circadian rhythm and stress-induced changes in hypothalamic content of corticotropin-releasing activity during postnatal development in the rat.


Evaluation of angiotension II metabolism in sheep by radioimmunoassay.


Role of catecholamines in stimulating the release of pituitary ovulating hormone(s) in rats.


The effect of tetanus toxin on neuromuscular transmission.


A sporadic case of juvenile amyotrophic lateral sclerosis; semi-quantitative and histo-enzymatical study of the denervated muscles.


Studies on the development of the gonads in embryos of the domestic pig (Sus scrofa dom. L.). IV. Cells containing PAS-positive grains in the differentiating testis anlage.


Histochemical studies on the action of aflatoxins. I. A cytological and cytochemical study of the liver of rats fed a diet contaminated with Aspergillus flavus.


Effect of cold exposure on serum thyrotropin.


Cortisol is secreted episodically by normal man.


Nasopharyngeal angiofibroma of the maxilla: report of case.


Four base-specific nucleoside 5'-triphosphatases in the subviral core of reovirus.


Hydrolysis of phospholipid esters localized in lysosomes and related structures of rat muscle cells in culture.


Strychnine, glycine and vertebrate postsynaptic inhibition.


Multiple sclerosis: pilot therapeutic trial of immunosuppressants.


Studies of pituitary-adrenocortical function in ulcerative colitis.


Adenine compounds on changes in optical density and motility of frog gastric mucosa.


Importance of aerobic and anaerobic metabolism in renal concentration and dilution.


Blood groups and their relation to serum phosphohydrolases.


Physiology of prostatic phosphohydrolase secretion.


A phylogenetic study of the renin-angiotensin system.


5'-nucleotidase of plasma membranes of the rat liver: studies on subcellular distribution.


Soluble and membrane-associated forms of acid phosphatase associated with the lysosomal fraction of rat liver.


Some properties and possible physiological functions of phosphotransferase activities of microsomal glucose-6-phosphatase.


Oligo- and polysaccharide inhibition of muscle transferase D phosphatase.


Clinical application of phosphohydrolase measurements in cancer.


Oxidative phosphorylation in fractionated bacterial systems. XLII. The effect of coupling factors on urea-treated particles from M. phlei.


Oxidative phosphorylation in fractionated bacterial systems. 43. Coupling factors associated with the NAD+ linked electron transport pathway.


The activation and inactivation of the acyl phosphatase activity of glyceraldehyde-3-phosphate dehydrogenase.


Analytical differential centrifugation: an analysis of the sedimentation properties of synaptosomes, mitochondria and lysosomes from rat brain homogenates.


Studies on myosin catalysis and modification.


Structural relations in aldolases purified from rat liver and muscle and Novikoff hepatoma.


Fructose diphosphatase from rabbit muscle. 3. Isolation of enzyme-substrate and enzyme-adenosine monophosphate complexes.


The identity of the aldolases isolated from rat muscle and primary hepatoma.


On the occurrence of respiratory components in calf thymus nuclei. II. Cytochrome oxidase activity.


The effect of starvation on the incorporation of palmitate into glycerides and phospholipids of rat liver homogenates. 1. Glyceride biosynthesis from glycerol phosphate and [1-(14)C]palmitate was studied in liver homogenates of rats that were fed ad libitum or starved for 36-40hr. The changes in enzyme activity were related to total DNA content or total liver homogenate as these were found to be equivalent and to be the most meaningful parameters. 2. In liver homogenates from fed rats, labelled palmitate was incorporated mainly into phosphatidate (58% of the total incorporation into lipids), diglycerides (25%) and triglycerides (16%), whereas monoglycerides, cholesterol esters and phospholipids other than phosphatidate were labelled only to a small extent. Addition of particle-free supernatant to full homogenates increased the total incorporation of palmitate by 45% and the pattern of incorporation altered to 53% incorporated into triglycerides, 24% into diglycerides and 17% into phosphatidate. This result suggested that, in liver homogenates, phosphatidate phosphohydrolase (EC 3.1.3.4) may be rate-limiting in the biosynthesis of glycerides via the glycerol phosphate pathway. 3. Upon starvation, the amount of palmitate incorporated per liver into total phospholipids plus glycerides was decreased to between 68% and 75% of that observed with fed animals. In homogenates from fed animals 41-44% of the labelled phospholipids plus glycerides was in glycerides; this value increased to between 63% and 75% with starved rats. Of the palmitate incorporated into total phospholipids, between 85% and 86% was found in phosphatidate, independent of the nutritional state of the animal. The ratio of palmitate incorporated into triglycerides/diglycerides rose from 0.7, obtained with fed rats, to 1.0 with starved animals. 4. These results indicate that starvation caused a decrease in the activity (per total liver) of acyl-CoA-glycerol phosphate acyltransferase(s) (EC 2.3.1.15) and an increase in the activity of acyl-CoA-diglyceride acyltransferase (EC 2.3.1.20). The largest change, however, seemed to be related to the increased activity of the phosphatidate phosphohydrolase in the particle-free supernatant. 5. The latter enzyme was assayed in the particle-free supernatant with membrane-bound phosphatidate as substrate. In starvation, the activity per total liver was increased to between 130% and 190% and the specific activity to between 180% and 320% of the values for fed rats.


Purine metabolism in germinating wheat embryos. 1. Both the acid-soluble fraction and the nucleic acid fraction of wheat embryos were extensively labelled after incubation for 6hr. in the presence of [8-(14)C]adenine. Subsequent incubation in the absence of labelled adenine resulted in no loss of radioactivity to the medium during a 48hr. period. Radioautography indicated that during this period there was a continuous increase in the radioactivity present in the acid-insoluble fractions of the root and leaf tissues relative to that present in the coleorhiza and coleoptile. 2. During incubation at 25 degrees there was a 26-fold increase in the activity of 3'-nucleotidase between 4hr. and 24hr.; the activities of enzymes hydrolysing AMP and IMP increased to a smaller extent. The activities of adenine phosphoribosyltransferase and hypoxanthine phosphoribosyltransferase increased three- to five-fold during incubation at 25 degrees for 24hr. 3. Adenosine kinase, inosine phosphorylase and 5-phosphoribosyl pyrophosphate synthetase activities were high in extracts from dry embryos and did not increase during 48hr. at 25 degrees . 4. The increase in 3'-nucleotidase activity was prevented by cycloheximide, cryptopleurine or incubation at 4 degrees , but not by actinomycin D; these treatments did not depress the activity of the other enzymes measured. 5. The results are discussed in relation to RNA translocation within the wheat embryo during germination.


Periodate oxidation studies of human pituitary follicle-stimulating hormone. A preparation of human pituitary follicle-stimulating hormone was subjected to periodate oxidation, borohydride reduction and acid hydrolysis. Comparison of the analysis of the remaining intact carbohydrate and amino acid units with the analyses of the original material and identification of the carbohydrate fragments permit some structural assignments to the molecule of follicle-stimulating hormone. The results of radioimmunological assay of fragments of the molecule of follicle-stimulating hormone suggest that, although the carbohydrate component is essential for biological activity, it is not a requirement for immunological activity, which appears to be a function of the protein moiety.


Metabolism of vitamin D. A new cholecalciferol metabolite, involving loss of hydrogen at C-1, in chick intestinal nuclei. 1. A comparison was made of the nature and intestinal intracellular distribution of the metabolites formed in vitamin D-deficient chicks from [4-(14)C]cholecalciferol and [1-(3)H]cholecalciferol. 2. The simultaneous administration of the two radioactive substances showed the presence in blood, liver, intestine, kidney and bone of cholecalciferol, its ester, 25-hydroxycholecalciferol and a further metabolite of cholecalciferol more polar than 25-hydroxycholecalciferol. The (3)H/(14)C ratios in these four radioactive components were the same as that of the dosed material (4.7:1) with the exception of the most polar material. The (3)H/(14)C ratio was lower in the fourth, most polar, metabolite (0.4:1-1.8:1) in all tissues examined, with the exception of blood. 3. In the chick intestine the polar metabolite accounted for almost 70% of the radioactivity in this tissue after a dose of 0.5mug. of [4-(14)C,1-(3)H]cholecalciferol. This polar metabolite from the intestine also had the lowest (3)H/(14)C ratio of all the tissues. It appears that in the chick intestine the polar metabolite reaches a maximum concentration of 1ng./g. of tissue, above which it cannot be increased irrespective of the dose of the vitamin. 4. The intestinal intracellular organelle with the highest concentration of (14)C radioactivity is the nucleus, and this radioactivity is almost entirely due to the polar metabolite with the lowered (3)H/(14)C ratio, in this case <0.2:1. It appears to be further localized in the chromatin of the nuclei. However, about half of the polar metabolite in the intestine is extranuclear. 5. Double-labelled 25-hydroxycholecalciferol was prepared and after its administration to vitamin D-deficient chicks the polar metabolite with the lowered (3)H/(14)C ratio was detected in liver, kidney, intestine, bone, muscle and heart. 6. None of the polar metabolite with the lowered (3)H/(14)C ratio was detected 16hr. after dosing with either the double-labelled vitamin or the double-labelled 25-hydroxycholecalciferol in blood and adipose tissue of vitamin D-deficient chicks, nor in the intestine, liver and kidney of supplemented birds. 7. The reasons for this loss of (3)H relative to (14)C are discussed in relation to possible chemical structures of this new polar metabolite.


Isolation of chick intestinal nuclei. Effect of vitamin D3 on nuclear metabolism. 1. Chick intestinal nuclei were isolated, with practically no contamination from other organelles and whole cells, by centrifugation through 2.4m-sucrose. 2. The proportions of RNA, DNA and protein of the isolated nuclei were unaffected by the vitamin D status of the birds. The RNA/DNA ratio was 0.15. 3. The incorporation of [5-(3)H]orotic acid into the rapidly labelled intestinal nuclear RNA, after a 10min. pulse of the orotic acid, was increased in vitamin D-deficient chicks only 10min. after a 125mug. dose of cholecalciferol. 4. There was no stimulation of the DNA-dependent RNA polymerase activity of the isolated nuclei from birds treated with cholecalciferol. 5. The results are discussed in relation to the changes occurring during the lag period, after administration of cholecalciferol and before Ca(2+) transport is detected, and the function of the vitamin.


A lipid intermediate in the synthesis of a poly-(N-acetylglucosamine 1-phosphate) from the wall of Staphylococcus lactis N.C.T.C. 2102. 1. The enzymic synthesis of the wall polymer poly-(N-acetylglucosamine 1-phosphate) in Staphylococcus lactis N.C.T.C. 2102 was studied by using UDP-[acetyl-(14)C]N-acetylglucosamine and the corresponding nucleotide containing (32)P. 2. Labelled material was extracted from the particulate enzyme preparation with butan-1-ol. Pulse-labelling experiments indicated that this material contained an intermediate in the biosynthesis. 3. The lipid intermediate was partially purified, and chemical and enzymic degradation showed that it was composed of N-acetylglucosamine 1-pyrophosphate in labile ester linkage to an organic-soluble alcohol, possibly a polyisoprenoid alcohol. The methanolysis of sugar 1-pyrophosphate derivatives, including nucleoside diphosphate sugars, is discussed in relation to degradation products obtained from the lipid. 4. The lipids from the particulate enzyme preparation probably contained another compound in which N-acetylglucosamine 1-phosphate is attached to an organic-soluble alcohol; this may participate in the biosynthesis of another polysaccharide. 5. The function of the lipid intermediate in polymer biosynthesis is discussed.


Pathways of glycogen synthesis in Novikoff ascites-hepatoma cells. Affinity of glucose, fructose and mannose for tumour hexokinase and their rates of phosphorylation at saturation concentration have been correlated with rates of glycogen synthesis by intact tumour cells at different concentrations of the three substrates. Competition experiments with one sugar labelled and the other sugar unlabelled indicate inhibition of glycogen synthesis by the sugar with a low K(m) for hexokinase. Glycogen synthesis from glucose 1-phosphate in aged cells and from nucleoside in freshly prepared cells is stimulated by fructose and inhibited by glucose. The decrease in glycogen formation from glucose 1-phosphate by oligomycin is partially overcome by increased fructose concentrations. These results are explained by an activation of alpha-glucan phosphorylase by fructose and an inhibition of this enzyme by glucose. It is suggested that differences in localization of glucose 6-phosphate, available to the intact cell in various ways, determine its transformation into glycogen by either the UDP-glucose-alpha-glucan glucosyltransferase reaction or by the alpha-glucan phosphorylase reaction.


One-electron reactions of some mitochondrial components studied by pulse radiolysis.


The long-wavelength absorption band of cytochrome c oxidase.


Phosphorylating respiratory membranes from Azotobacter vinelandii.


Intramitochondrial distribution of cytochrome P-450 in ox adrenal cortex.


Cytochrome c redox potentials as a function of the energy state in chromatophores.


The effects of calcium ions and adenosine diphosphats on the activity of nicotinamide-adenine dinucleotide-linked isocitrate dehydrogenase of muscle.


Changes in subcellular distribution of enzymes during respiratory adaptation of Saccharomyces carlsbergensis.


Studies on the phospholipids of yeast mitochondria.


The stereospecificity of tritium distribution in [1-3H]- and [1,2-3H2]-cholesterol and -cholecalciferol.


Characterization of rapidly labelled rat liver ribonucleic acid showing high affinity for columns of methylated albumin on kieselguhr. Most of the rapidly labelled RNA from rat liver submitted to column chromatography on methylated albumin on kieselguhr remains tightly bound to the column and can only be recovered by elution with m-ammonia. The tightly bound RNA is composed mainly of DNA-like RNA. The binding capacity is dependent not only on base composition but also on molecular size: the heavier RNA molecules show a greater affinity to the column than do the lower-molecular-weight components. Rapidly labelled mouse liver and Saccharomyces cerevisiae RNA show similar behaviour to rat liver RNA on columns of methylated albumin on kieselguhr.


Isolation and partial characterization of apiogalacturonans from the cell wall of Lemna minor. 1. A mild, reproducible extraction procedure, using 0.5% ammonium oxalate, was developed for the isolation of polysaccharides containing d-apiose from the cell wall of Lemna minor. On a dry-weight basis the polysaccharide fractions extracted with ammonium oxalate made up 14% of the material designated cell walls and contained 20% of the d-apiose originally present in the cell walls. The cell walls, as isolated, contained 83% of the d-apiose present in L. minor. 2. After extraction with ammonium oxalate, purified polysaccharides were obtained by DEAE-Sephadex column chromatography and by fractional precipitation with sodium chloride. With these procedures the material extracted at 22 degrees C could be separated into at least five polysaccharides. On a dry-weight basis two of these polysaccharides made up more than 50% of the material extracted at 22 degrees C. There was a direct relationship between the d-apiose content of the polysaccharides and their solubility in sodium chloride solutions; those of highest d-apiose content were most soluble. 3. All the polysaccharides isolated appeared to be of one general type, namely galacturonans to which were attached side chains containing d-apiose. The d-apiose content of the apiogalacturonans varied from 7.9 to 38.1%. The content of esterified d-galacturonic acid residues in all apiogalacturonans was low, being in the range 1.0-3.5%. Hydrolysis of a representative apiogalacturonan with dilute acid resulted in the complete removal of the d-apiose with little or no degradation of the galacturonan portion. 4. Treatment of polysaccharide fractions with pectinase established that those of high d-apiose content and soluble in m-sodium chloride were not degraded, whereas those of low d-apiose content and insoluble in m-sodium chloride were extensively degraded. When the d-apiose was removed from a typical pectinase-resistant polysaccharide, the remainder of the polysaccharide was readily degraded by this enzyme. 5. Periodate oxidation of representative polysaccharide fractions and apiogalacturonans and determination of the formaldehyde released showed that about 50% of the d-apiose molecules were substituted at either the 3- or the 3'-position.


The activity of the pituitary cells of the teleost Poecilia during the gestation cycle and the control of the gonadotropic cells.


Microscopic examination of parathion activating microsomes from American cockroach fat body.


Histochemical studies of the neurosecretory system of Chirocephalus diaphanus Prévost (Crustacea: Anostraca).


Action of nicotine on neural synaptic transmission in the American cockroach.


Release of a synaptically active material by nicotine in the central nervous system of the American cockroach.


Differential responses to various ACTH-releasing stimuli in rats with hypothalamic implants of corticosteroids.


Metabolic and virologic studies in primate liver organ cultures.


Effects of isolation of medial basal hypothalamus on pituitary-adrenal and pituitary-ovarian functions.


A new pressor factor in extract of rat aorta wall.


DNA synthesis in EB virus-containing Burkitt lymphoma cultures during a temperature cycling procedure.


Characterization of reptilian cell lines established at incubation temperatures of 23 to 36 degrees.


The effect of parenteral and oral immunization on encephalomyocarditis infection in mice.


Herpesvirus hominis types I and II: a specific microindirect hemagglutination test.


Induction of interferon in lymphoblastoid cell lines.


Differential decrease of "shuttle" enzymes of extramitochondrial NADH2 oxidation of heart muscle during progressive thiamine deficiency.


Non-antigen-antibody precipitin reactions observed with dextran sulfate, DEAE-dextran, antibiotics, proteins, and phospholipids.


Lactate dehydrogenase in the rat mammary gland.


EBV antibodies in family contacts of patients with infectious mononucleosis.


Strontium inhibition of vitamin D3-induced calcium-binding protein (CaBP) and calcium absorption in chick intestine.


Carcinogenicity testing of selected food additives by parenteral administration to infant Swiss mice.


Generalized muscular stiffness, fasciculations, and myokymia of peripheral nerve origin.


Gluconeogenesis in the kidney cortex. Flow of malate between compartments. 1. Kidney-cortex slices from starved rats were incubated with l-[U-(14)C]lactate or l-[U-(14)C]malate plus unlabelled acetate and the specific radioactivity of the glucose formed was determined. In parallel experiments the specific radioactivity of the glucose formed from [1-(14)C]acetate plus unlabelled l-lactate and l-malate was determined. 2. By analytical methods the major products formed from the substrates were measured. The glucose formed was purified by paper chromatography for determination of specific radioactivity. 3. The specific radioactivity of the glucose formed from l-[U-(14)C]lactate agrees with predictions of a model based on interaction of the gluconeogenic and the oxidative pathways. 4. The specific radioactivity of the glucose formed from l-[U-(14)C]malate agrees with the predicted value if rapid malate exchange between the cytosol and mitochondria is assumed. 5. The rate of malate exchange between compartments was estimated to be rapid and at least several times the rate of glucose formation. 6. The specific radioactivity of the glucose formed from [1-(14)C]acetate plus unlabelled l-lactate or l-malate agrees with the predictions from the model, again assuming rapid malate exchange between compartments. 7. Malate exchange between compartments together with reversible malate dehydrogenase activity in the mitochondria and cytosol also tends to equilibrate isotopically the NADH pool in these compartments. (3)H from compounds such as l-[2-(3)H]lactate, which form NAD(3)H in the cytosol, appears in part in water; and (3)H from dl-beta-hydroxy[3-(3)H]butyrate, which forms NAD(3)H in the mitochondria, appears in part in glucose, largely on C-4.


Spinal afferents to SI and SII of the rat thalamus.


Interferon response of lymphocytes in disorders with decreased resistance to infections.


Synaptic transmission in the degenerating lateral geniculate nucleus. An ultrastructural and electrophysiological study.


Isolation of membrane-associated tumour-specific antigen from an aminoazo-dye-induced rat hepatoma.


Malignant and transforming activity of Rous sarcoma virus. II. The study of variants of Rous sarcoma virus isolated from mouse tumours.


Malignant and transforming activity of Rous sarcoma virus. I. Malignant effect of Rous sarcoma virus.


Studies on group-specific antigens in tumours induced with avian tumour viruses in rats.


Studies on Murine Sarcoma Virus; a morphological comparison of tumorigenesis by the Harvey and Moloney strains in mice, and the establishment of tumor cell lines.


Analysis of the neutralizing activity in nasal wash and serum following intranasal vaccinaion with inactivated type 13 rhinovirus.


Electrical transmission among neurons in the buccal ganglion of a mollusc, Navanax inermis. The opisthobranch mollusc, Navanax, is carnivorous and cannibalistic. Prey are swallowed whole by way of a sudden expansion of the pharynx. The buccal ganglion which controls this sucking action was isolated and bathed in seawater. Attention was focused upon 10 identifiable cells visible on the ganglion's rostral side. Two cells were observed simultaneously, and each was penetrated with two glass microelectrodes, one for polarizing the membrane and the other for recording membrane potential variations. The coupling coefficients for direct current flow and action potentials of several identified cells were tabulated. Attenuation was essentially independent of the direction of current flow, but depended upon the relative size of the directly and indirectly polarized cells. The attenuation of subthreshold sinusoidally varying voltages increased with frequency above about 1 Hz. The coupling coefficient for spikes was lower than for DC due to greater high frequency attenuation. There is considerable similarity in the spontaneous PSP's of all cells, which is not due to the electrical coupling but to input from a common source. The 10 cells were not chemically interconnected but some were electrically connected to interneurons which fed back chemically mediated PSP's. The feedback can be negative or positive depending upon the membrane potential of the postsynaptic cell. We conclude that electrical coupling among the 10 cells plays a minor role in sudden pharyngeal contractions but that the dual electrical-chemical coupling with interneurons may be important in this respect.


Late 19S rabbit antibody neutralization test for differentiating herpes simplex virus types 1 and 2.


Measurement of antibodies to herpesvirus types 1 and 2 in human sera.


Purification and serologic tests with adenovirus 2 virion and T antigens.


The neuromyopathy of vincristine in man. Clinical, electrophysiological and pathological studies.


The neuromyopathy of vincristine in the guinea pig. An electrophysiological and pathological study.


Experiences in laboratory diagnosis of parainfluenza viruses in routine medical practice.


The epidemiology of cholera in Calcutta as noted by biotyping the isolates.


Endogenous collagenase in periodontal disease.


A synthetic polypeptide--beta 1-24 corticotropin--in the depot form. I. Adrenocorticotropic and pituitary responsiveness following long-term intermittent administration.


A synthetic polypeptide--beta 1-24 corticotropin--in the depot form. II. Clinical efficacy of intermittent therapy in asthmatic patients.


The disappearance rate of corticosterone from the blood of ACTH-treated rats.


Influence of pituitary hormones (ACTH and ACTH analogues) and corticosteroid on the central nervous system in rats and chicks. Electrophysiological and behavioural studies.


Clostridium welchii brain abscess following craniotomy for meningioma: a case report.


Further studies on the hypophyseal--adrenocortical response to various stressing procedures in ACTH-treated rats.


A simplified method for the determination of vitamins D2 and D3 in water-dispersible preparations by gas-liquid chromatography.


Stress ulcers.

Stress ulcers.


Adenosine 3',5'-monophosphate, adrenocorticotropic hormone, and adrenocortical cytosol protein synthesis. In cultures of mouse adrenocortical tumor cells (Sato's minimal deviation Y-1 clonal strain), the acceleration of steroid biosynthesis after exposure to adrenocorticotropic hormone or cyclic adenosine 3',5'-monophosphate is maximum within 15 to 60 minutes and precedes any significant increase in labeling of protein with [4,5-(3)H]leucine. However, when cytosol proteins are separated by acrylamidegel electrophoresis, rapid changes in the amount and labeling of several protein fractions are evident in less than 30 minutes and are no longer evident within 60 minutes. This finding supports the proposal that the effects of tropic hormones and their intracellular mediators involve rapid selective effects on protein synthesis.


Phage-typing of Klebsiella strains isolated from the faeces of infants and children.


An electron microscopic study of the changes in ACTH- and FSH-producing cells during development of methylandrostenediol hypertension in the rat.


Lung disease in Appalachian soft-coal miners.


The isolation of infectious bovine rhinotracheitis virus from cattle in Northern Queensland.


Histochemistry of the thymus and a thymoma.


Glutamine phosphoribosylpyrophosphate amidotransferase. Catalytic and conformational heterogeneity of the pigeon liver enzyme.


Characterization of a fluorescent complex between auramine O and horse liver alcohol dehydrogenase.


Exonuclease (phosphodiesterase) from the testes of Chinook salmon (Oncorhynchus tschawytscha).


Formation of (+)-cis-2,3-dihydroxy-1-methylcyclohexa-4,6-diene from toluene by Pseudomonas putida.


Regulation of the biosynthesis of purine nucleotides in Schizosaccharomyces pombe. I. Properties of the phosphoribosylpyrophosphate: glutamine amidotransferase of the wild strain and of a mutant desensitized towards feedback modifiers.


Regulation of skeletal muscle phosphorylase phosphatase activity. I. Kinetic properties of the active and inactive forms.


Regulation of skeletal muscle phosphorylase phosphatase activity. II. Interconversions.


Purification and properties of clostridium welchii phospholipase C.


Hydrolysis, racemization and absolute configuration of ureidoglycolate, a substrate of allantoicase.


Radiation effects on NADH.

Radiation effects on NADH.


Allotransplantation of joints.

Allotransplantation of joints.


The localization of cytochrome c oxidase activity during mitochondrial specialization in spermiogenesis of prosobranch snails.


Ultrastructural and histochemical studies on the epithelium revestment layer in the tube feet of the starfish Asterina stellifera.


Interferon production by mouse peritoneal cells.


Radiation, infection, and macrophage function. I. Effects of whole body radiation on dermal tuberculous lesions in rabbits: development, histology, and histochemistry.


Leukemoid changes induced in germfree and conventional rats by Rous sarcoma virus.


Metabolic control in isolated brown fat cells.


A mechanism for hormonal activation of lipolysis and respiration in free brown fat cells.


Effects of angiotensin on the autonomic nervous system.


Vestibular dysfunction in schizophrenia and childhood autism.


A night hospital program.

A night hospital program.


Permissive effects of hormones on lypolysis.


Social clubs help prevent readmission.

Social clubs help prevent readmission.


Mediators of tissue damage in leukocyte lysosomes. X. Further studies on human granulocyte elastase.


NAD biosynthesis in polymorphonuclear leucocytes of guinea-pigs. I. Nicotinic acid pathway of NAD synthesis in cell-free preparations.


Comparative morphologic and functional studies of neptunium-induced fatty livers in rats.


Plasma renin in congestive heart failure in man.


Non-chromaffin paragangliomas. Brief review and clinical-radiological follow-up on nine cases.


FSR for biologists.

FSR for biologists.


Immunologic distinction between infectious and serum hepatitis.


Cochlear damage from ototoxic antibiotics by intratympanic application.


Formation of virus-like particles by bone cells in mice with a high incidence of spontaneous leukemia. Bone samples from potentially leukemic and leukemic mice revealed numerous 90-to 110-nanometer particles morphologically identical to murine leukemia virus. Particles were observed budding from plasma membranes of osteocytes and osteoblasts but were most numerous in osteocyte lacunae. Particles were not observed in bone samples from mice which rarely develop leukemia.


Haem-plane orientation in single crystal of bonito cytochrome c.


ACTH or corticosteroids? A critical review of results and possibilities in the treatment of severe chronic disease.


A radioisotopic method for measuring the formation of adenosine 3',5'-cyclic monophosphate in incubated slices of brain.


Reconstruction of the glycerolphosphate cycle in suspensions of rat brain mitochondria.


Mycoecology on serpentine soil.

Mycoecology on serpentine soil.


Sleep disturbance and serum CPK activity in acute psychosis.


Sleep during alcohol intake and withdrawal in the chronic alcoholic.


The premature deposition or lysis of glycogen in livers of fetal rats injected with hydrocortisone or glucagon.


The effect of an interferon inducer on influenza virus. The double-stranded complex of polyriboinosinic acid and polyribocytidilic acid (poly I.poly C) was shown to inhibit effectively the infection of human cells with 4 common human respiratory pathogens-influenza A2, rhinovirus 13, respiratory syncytial and parainfluenza-1 viruses. This protection was observed with levels of poly I.poly C which did not induce the release of detectable amounts of interferon. Mice could be protected from pulmonary influenza A2 infections by intranasal instillation of poly I.poly C before challenge with virus. Administration of poly I.poly C directly to the respiratory tract was found superior to systemic administration in the case of the pulmonary infection studied.


Electron spin resonance and optical studies of the interaction between NO2 and unsaturated lipid components.


Steroid biosynthetic enzymes and the regulation of mammalian reproduction.


Conditions simulating metastatic lesions of the brain. Report of eight cases.


Theca cell tumor of the ovary associated with pregnancy.


Protection of rabbits against experimental rabies of poly I-poly C.


Chemotactic factor associated with invasion of cancer cells.


Fatty acid mobilization in obese mice.

Fatty acid mobilization in obese mice.


New light-induced EPR signal in Anacystis nidulans.


Electron microscopic and histochemical counterparts of renal glomerular damage in experimental crush syndrome in white rat.


Activity of certain enzymes in blood plasma and dialyzing fluid in patients with renal failure treated with peritoneal dialysis.


Mössbauer spectroscopy of haem proteins.


Magnetic resonance studies of enzyme-substrate complexes with paramagnetic probes as illustrated by creatine kinase.


Heart glycosides in poliovirus host cell interaction. 3. Chemical structure and activity.


New selective medium for the isolation of Clostridium perfringens.


Bovine infectious keratoconjunctivitis: interactions of Moraxella bovis and infectious bovine rhinotracheitis virus.


Viral antibody patterns in laboratory dogs with respiratory disease.


Influence of peptides on the output of noradrenaline from isolated rabbit hearts.


The challenge of amoebiasis: understanding pathogenic mechanisms.


Histochemical differentiation of red and white muscle fibers.


Experimental Herpes-T infection in hamsters.


Comparison of stage four and 1-rem sleep deprivation.


Reactions of the rat and hamster endocrine system to aminoglutethimide.


Distribution of a porcine adenovirus after inoculation of experimental animals.


Pathogenesis of chronic mouse cytomegalovirus infection in submaxillary glands of C3H mice.


Effects of pesticides on nitrite oxidation by Nitrobacter agilis. The influence of pesticides on the growth of Nitrobacter agilis in aerated cultures and on the respiration of N. agilis cell suspensions and cell-free extracts was studied. Two pesticides, aldrin and simazine, were not inhibitory to growth of Nitrobacter, but five compounds [isopropyl N-(3-chlorophenyl) carbamate (CIPC), chlordane, 1,1-dichloro-2,2-bis (p-chlorophenyl) ethane (DDD), heptachlor, and lindane] prevented growth when added to the medium at a concentration of 10 mug/ml. Whereas CIPC and eptam prevented nitrite oxidation by cell suspensions, the addition of DDD and lindane resulted in only partial inhibition of the oxidation. Heptachlor and chlordane also caused only partial inhibition of oxidation, but were more toxic with cell-free extract nitrite oxidase. None of the pesticides inhibited the nitrate reductase activity of cell-free extracts, but most caused some repression of cytochrome c oxidase activity. Heptachlor was the most deleterious compound.


Hydrolysis of conjugated bile acids by cell-free extracts from aerobic bacteria. By means of an aerobic enrichment culture technique, several bacteria that hydrolyze conjugated bile acids and modify the formed deconjugates were isolated from feces of man, rat, and chicken and from soil. Hydrolase activity was intracellular and extractable, and the yield of the enzymes was increased by adding the conjugated bile acids to the culture media. The hydrolase from bacterium of human origin was stable, having a pH optimum at about 7.0. All bile acid conjugates were hydrolyzed linearly as a function of time.


Pathogenicity of Clostridium perfringens for germ-free guinea pigs after oral ingestion. Germ-free guinea pigs died with signs and lesions of acute enterotoxemia after oral ingestion of C. perfringens types B, C, D, and E. The signs and lesions observed resembled those seen in acute enterotoxemia of sheep and cattle and the naturally occurring disease seen in "ex-germ-free" guinea pigs. C. perfringens type A was found to be innocuous. Conventional guinea pigs did not become ill after ingestion of any of the five toxigenic types.


Comparative poliovirus permeability of silver, polycarbonate, and cellulose membrane filters. Three types of filters, silver, polycarbonate, and cellulose, were evaluated for permeability toward poliovirus suspended in water, salt-containing, and proteinaceous solvents. The ability of virus to pass through cellulose filters depended on the suspending medium; virus did not pass through cellulose filters in either water or salt solution, whereas the use of protein solutions increased filterability. The virus permeability of both the silver and polycarbonate filters was independent of the suspending medium. Apparently, pore size alone determined their permeability toward poliovirus, and electrostatic forces between filters and the particles being filtered did not appear to play a significant role. Both the silver and polycarbonate filters appear to be promising tools for the separation of viruses from contaminating bacteria and fungi.


Serratia marcescens: biochemical, serological, and epidemiological characteristics and antibiotic susceptibility of strains isolated at Boston City Hospital. The biochemical, serological, and epidemiological characteristics of 95 strains of Serratia marcescens isolated at the Boston City Hospital were examined. Several strains were shown to be endemic, and the majority of isolates were cultured from urine or respiratory secretions. Serratia species were highly resistant to polymyxin B and the cephalosporins, and various proportions were also resistant to other antibiotics including kanamycin, but all of the isolates were sensitive to gentamicin. The appearance of resistance to kanamycin and nalidixic acid among endemic strains was demonstrated. The nosocomial nature of Serratia infections, particularly those involving the urinary tract, was confirmed. Many clinical bacteriology laboratories currently fail to identify the nonpigmented strains.


Growth from spores of Clostridium perfringens in the presence of sodium nitrite. The method by which sodium nitrite may act to prevent germination or outgrowth, or both, of heat-injured spores in canned cured meats was investigated by using Clostridium perfringens spores. Four possible mechanisms were tested: (i) prevention of germination of the heat-injured spores, (ii) prior combination with a component in a complex medium to prevent germination of heat-injured spores, (iii) inhibition of outgrowth of heat-injured spores, and (iv) induction of germination (which would render the spore susceptible to thermal inactivation). Only the third mechanism was effective with the entire spore population when levels of sodium nitrite commercially acceptable in canned cured meats were used. Concentrations of 0.02 and 0.01% prevented outgrowth of heat-sensitive and heat-resistant spores, respectively. Nitrite-induced germination occurred with higher sodium nitrite concentrations.


Revised data on (35S)-atractyloside binding to mitochondrial membranes.


Uncoupling of oxygen activation from hydroxylation in a bacterial salicylate hydroxylase.


Stimulation of lac mRNA synthesis by cyclic AMP in cell free extracts of Escherichia coli.


The interactions of copper and a3 in cytochrome oxidase.


Evidence of mitochondrial synchrony in synchronous cell cultures of yeast.


Inactivation and repression by ammonium of the nitrate reducing system in chlorella.


The effect of catabolite repression and of cyclic 3',5' adenosine monophosphate on the translation of the lactose messenger RNA in Escherichia coli.


Acylation of lysine and of arginine-rich histones with carbamyl phosphate and 1,3 diphosphoglycerate.


Abscisic acid induced changes in nucleotide composition of rapidly labelled RNA species of pear embryos.


Studies on the nonspecific phosphodiesterase activity of splenic acid deoxyribonuclease.


Absorption spectrophotometry of perfused rat liver applied to fructose-induced inhibition of respiration.


Somatomotor inhibition and excitation by forebrain stimulation during sleep and wakefulness: orbital cortex.


Responses of neurons in the raphe nuclei to serotonin, norepinephrine and acetylcholine and their correlation with an excitatory synaptic input.


On the interaction of picrotoxin with GABA and glycine in the spinal cord.


Co-ordination between membrane phospholipid synthesis and accelerated biosynthesis of cytoplasmic ribonucleic acid and protein. 1. The rate of synthesis of membrane phospholipid was studied in rat liver and seminal vesicles by following the incorporation of [(32)P]orthophosphate, [(14)C]choline and [(14)C]glycerol. Particular emphasis was laid on the endoplasmic reticulum, which was fractionated into smooth microsomal membranes, heavy rough membranes, light rough membranes and free polyribosomes. 2. Phospholipid labelling patterns suggested a heterogeneity in the synthesis and turnover of the different lipid moieties of smooth and rough endoplasmic membranes. The major phospholipids, phosphatidylcholine and phosphatidylethanolamine, were labelled relatively rapidly with (32)P over a short period of time whereas incorporation of radioisotope into the minor phospholipids, sphingomyelin, lysolecithin and phosphatidylinositol proceeded slowly but over a longer period of time. 3. The incorporation of orotic acid into RNA and labelled amino acids into protein of the four submicrosomal fractions was also studied. 4. Rapid growth of the liver was induced by the administration of growth hormone and tri-iodothyronine to hypophysectomized and thyroidectomized rats and by partial hepatectomy. Growth of seminal vesicles of castrated rats was stimulated with testosterone propionate. 5. The rate of labelling of membrane phospholipids was enhanced in all major subcellular particulate fractions (nuclear, mitochondrial and microsomal) during induced growth. However, it was in the rough endoplasmic reticulum that the accumulation of phospholipids, RNA and protein was most marked. The effect of hormone administration was also to accelerate preferentially the labelling with (32)P of sphingomyelin relative to that of phosphatidylcholine or phosphatidylethanolamine. 6. Time-course analyses showed that, in all four growth systems studied, the enhancement of the rate of membrane phospholipid synthesis coincided with the rather abrupt increase in the synthesis of RNA and protein of the rough endoplasmic reticulum. Growth hormone and tri-iodothyronine administered to hypophysectomized rats had additive effects in all the biosynthetic processes. The latent period of action of each hormone was maintained so that two waves of proliferation of endoplasmic reticulum occurred if the hormones were administered simultaneously. 7. It is concluded that there is some mechanism in the cell that tightly co-ordinates the formation of membranes, especially those of the endoplasmic reticulum, when an increased demand is made for protein synthesis.


Labyrinthine and neck reflexes recorded from spinal single motoneurons in the cat.


Amoebiasis cutis following surgical drainage of a large hepatic, subphrenic and perinephric abscess.


Rhinovirus infections in Glasgow in 1962-66.


A mixed longitudinal study on skeletal maturation in healthy Finnish children aged 1 to 5 years.


Increase of serotonin in the adductors of Anodonta cygnea L. (Pelecypoda) relaxed by nerve stimulation and in relation to the periodic activity.


Prevalence and duration of postnatally acquired human cytomegalovirus  infection.


Characteristics of transmission ilure in the squid stellate ganglion: a study of a simple habituating system.


Control of prolactin release and its role in color change in the teleost Gillichthys mirabilis.


A re-investigation of evidence for cholinergic neuromuscular transmission in insects.


Properties of temperature-sensitive mutants of the KENYA 3/57 strain of foot-and-mouth disease virus.


Inhibition of poliovirus RNA synthesis by supraoptimal temperatures.


Effect of fasting and of phenobarbital on the distribution and latency of glucose-6-phosphatase in microsomal sub-fractions of rat liver.


Plasma corticosterone and circadian rhythm in generation of the Arneil-Dekanski factor.


Interrelations of some pituitary hormones.


Shared viral antigen of mammalian leukaemia viruses.


The carrier state in cattle in foot-and-mouth disease.


Transitory liver antigens and primary hepatoma in man and rat.


Experiences in a long-term cultivation of own human diploid cell strains in synthetic medium with growth promoting proteins.


Experience with production of attenuated poliovirus in monkey kidney and WI-38 cells.


Utilization and control of BHK cells in inactivated foot-and-mouth disease vaccine production.


Behaviour of enzyme systems in cercopithecus and rhesus kidney cells in consequence of SV40 virus action.


Enzymatic cell-virus relations: lactate dehydrogenase and glutamic-oxaloacetic transaminase in normal and infected tissue cultures.


The production of large gnotobiotic animals and their use in the preparation of virus reference sera.


Enhancement of viral infectivity with DEAE-dextran: application to development of vaccines.


Testing of vaccines for avian leukosis viruses.


Animal cell strains as substitutes for primary monkey cultures in testing Salk and other inactivated vaccines for persistent viruses.


Semicontinuous cultivation of Bordetella pertussis.


The preparation of viral vaccines containing purified extracted antigens.


The relevance of agglutinogenic types of Bordetella pertussis in the assay of pertussis vaccines.


Experiences with a new combined vaccine against B. parapertussis, Diphtheria, tetanus and B. pertussis employed in a field trial in Prague 10.


The role of the serologic type in experimental immunity against B. Pertussis.


SV 40 replication in presence of monkey kidney antibodies.


The use of an in vitro test for the estimation of presence of the specific tumor antigen of the transplantation type induced by SV 40 virus.


Neutralizing antibodies to simian virus 40 (SV 40) in human sera.


Studies with SV 40--adeno 7 hybrid virus.


Adventitious agents in African green monkey kidney tissue cultures used for the production of live attenuated poliomyelitis vaccine.


Presence of viral genome in Chinese hamster embryonic cell transformed in vitro with Bryan strain of Rous sarcoma virus (B-RSV).


Establishment and maintenance of a specific pathogen free (SPF) flock of white leghorn chickens.


Problems associated with the study of spontaneous mutation towards guanidine resistance in poliovirus.


A consideration of the cell substrates used in human virus vaccine preparation.


Enzyme activity during the growth and aging of human cells.


Relative stability of the characteristic sulfur and doublet resonances in x-irradiated native proteins as measured with ESR.


Distinctive features and comparative value of the genetic markers defining the behaviour of poliovirus towards different selective inhibitors.


Late effects of x-irradiation in chemically protected mice: alteration of tumor incidence after AET-treatment.


EPR of fast repair reactions by sulfhydryl and disulfide compounds in aqueous solutions.


Current use of hypnotic drugs--is it rational?


6oCo gamma-radiolysis of liquid formamide: gaseous and high-boiling stable products.


Selective tosylation of adenosine 5'-monophosphate.


The stapediovestibular joint: normal structure and pathogenesis of otosclerosis.


Three dimensional observation of the inner ear with the scanning electron microscope.


Ectopic hormone production in cancer and its implication for basic research on abnormal growth.


The evolution of Cushing's syndrome as a clinical entity.


Surgical consideration in disorders of the horizontal and vertical portions of the facial nerve.


Influence of gibberellic acid on the incorporation of 8-14C adenine into adenosine 3',5'-cyclic phosphate in barley aleurone layers.


The intracellular distribution of the latent hexokinase in brain.


The enzymatic synthesis of N-methylalanine.


Stimulation by N6,O2'-dibutyryl adenosine 3',5'-cyclic-phosphate of amino acid transport, protein synthesis, and chondroitin sulfate synthesis in embryonic bone in vitro contrasted with the effects of thyroid hormones.


The metabolism of adenosine 3',5'-cyclic phosphate. I. Method for the determination of adenyl cyclase and some properties of the adenyl cyclase isolated from the rat kidney.


The effect of light upon ribonuclease activity of etiolated Lupinus albus hypocotyls.


The use of polyoma virus for studies on genetic regulation in mammalian cells.


The metabolism of adenosine 3',5'-cyclic phosphate. II. Some properties of adenosine-3',5'-cyclic-phosphate phosphodiesterase from the rat kidney.


The localization of the hemoglobin messenger RNA on the 40-S ribosomal subunit of rabbit reticulocyte polysomes.


Waldenström's macroglobulinemia: correlation between expanded plasma volume and increased serum viscosity.


Effects of hypophysectomy and salinity change on plasma cortisol concentration in the Japanese eel, Anguilla japonica.


Control of deoxyribonucleic acid and ribonucleic acid synthesis in pyrimidine-limited Escherichia coli. The effects of pyrimidine limitation on chromosome replication and the control of ribosomal and transfer ribonucleic acid syntheses were investigated. Chromosome replication was studied by autoradiography of (3)H-thymine pulse-labeled cells. Pyrimidine limitation did not affect the fraction of cells incorporating radioactive thymine during a short pulse, indicating that when growth is limited by the supply of pyrimidine, the time required for chromosome duplication increases in proportion to the time required for cell duplication. Control of ribosomal RNA and transfer RNA syntheses was examined by chromatographing cell extracts on methylated albumin kieselguhr columns. When growth was controlled by carbon-nitrogen limitation, the ratio of tRNA to total RNA remained roughly constant at growth rates above 0.5 doublings per hour. During pyrimidine limitation, however, the control of rRNA synthesis was apparently dissociated from the control of tRNA synthesis: the ratio of tRNA to total RNA increased as the growth rate decreased.


Stimulation of galactokinase synthesis in Escherichia coli by adenosine 3',5'-cyclic monophosphate. Adenosine 3',5'-cyclic monophosphate (cyAMP) stimulates the rate of synthesis of galactokinase in glycerol-grown Escherichia coli both when production of the enzyme is induced by d-fucose and when it is repressed by glucose in the presence of inducer. cyAMP also stimulates the synthesis of galactokinase in constitutive strains B78A (R(-)) and R10 (O(c)), and overcomes the transient repression of galactokinase synthesis caused by glucose.


Endonuclease from Micrococcus luteus which has activity toward ultraviolet-irradiated deoxyribonucleic acid: purification and properties. An endonuclease purified approximately 3,200-fold from Micrococcus luteus is active on native ultraviolet-irradiated deoxyribonucleic acid (DNA), but is inactive on unirradiated native or denatured DNA and has no activity toward irradiated denatured DNA. The major type of lesion for the nucleolytic activity is the cyclobutane pyrimidine dimer. The enzyme makes a number of single-strand breaks approximately equal to the number of dimers, but dimers are not excised. This endonuclease-a small molecular weight protein-therefore has all the attributes hypothesized for the first enzyme in the sequential steps in repair of DNA in vivo. Another paper shows that the endonuclease is able to reactivate ultraviolet-irradiated transforming DNA.


Masking of Bacillus megaterium KM membrane reduced nicotinamide adenine dinucleotide oxidase and solubilization studies. Solubilization of a reduced nicotinamide adenine dinucleotide (NADH)-2,6 dichlorophenol indophenol (DCIP) oxidoreductase associated with the membrane NADH oxidase system of Bacillus megaterium KM was effected by treatment with 0.2% sodium deoxycholate, 8 m urea, or buffer (pH 9.0) in the presence of ethyl-enediaminetetraacetate. These treatments inactivated membrane NADH oxidase. It was found that membrane NADH oxidase and NADH-DCIP oxidoreductase were masked in membranes. Several procedures, including brief sonic oscillation, treatment with 0.05% deoxycholate, prolonged stirring at 4 C with 10% glycerol, and washing in the absence of Mg(2+), unmasked the oxidase and oxidoreductase activities. It was necessary to study the masking and unmasking of these activities to quantitate adequately the effects of solubilization procedures. Further information on the localization of oxidase and oxidoreductase in subcellular fractions and the effects of electron transport inhibitors on NADH oxidation was also obtained.


Chromatography of microbial lipids by centrifugation through microparticulate gel. An improved apparatus and a procedure are described by which the migration of sample components in column chromatography is accelerated by centrifugal force, thereby making it possible to use beds of densely packed gel prepared with ultrafine silica. This technique was used to resolve components of certain lipid mixtures where other methods have failed, and it has been found generally useful as an adjunct to other methods for the fractionation of lipids. Biologically active phosphoglycolipids from Mycobacterium tuberculosis and a phosphatidylglycerol-like substance from Mycoplasma pneumoniae which formed single spots on thin-layer chromatographic plates were each found to contain a major and several minor components by centrifugal chromatography. The method enabled us to isolate individual components of Wax D from M. tuberculosis rather than a spectrum of components. Minor components were resolved which, although present in insufficient quantity to influence results of chemical analyses, may be responsible for biological activity. The apparatus provides an essentially closed system which reduces highly volatile solvents to minimal evaporation during the chromatographic process. Samples are applied in solution and are not allowed to dry on the columns until after separation has been achieved. Consequently, polar, labile microbial lipids can be resolved without the use of harsh reagents which destroy some of their properties. Single components may be harvested by cutting and removing appropriate segments of the larger chromatograms or by eluting them from the columns.


Endonuclease from Micrococcus luteus which has activity toward ultraviolet-irradiated deoxyribonucleic acid: its action on transforming deoxyribonucleic acid. An endonuclease purified from Micrococcus luteus makes single-strand breaks in ultraviolet (UV)-irradiated, native deoxyribonucleic acid (DNA). The purified endonuclease is able to reactivate UV-inactivated transforming DNA of Haemophilus influenzae, especially when the DNA is assayed on a UV-sensitive mutant of H. influenzae. After extensive endonuclease action, there is a loss of transforming DNA when assayed on both UV-sensitive and -resistant cells. The endonuclease does not affect unirradiated DNA. The results indicate that the endonuclease function is involved in the repair of biological damage resulting from UV irradiation and that the UV-sensitive mutant is deficient in this step. We interpret the data as indicating that the various steps in the repair of DNA must be well coordinated if repair is to be effective.


D-glucaric acid and galactaric acid catabolism by Agrobacterium tumefaciens. Cell-free extract (crude extract) of Agrobacterium tumefaciens grown on d-glucuronate or d-glucarate converts d-glucarate and galactarate to a mixture of 2-keto-3-deoxy- and 4-deoxy-5-keto-d-glucarate. These compounds are then converted by partially purified crude extract to an intermediate tentatively identified as 2,5-diketoadipate. The same enzyme preparation further decarboxylates this intermediate to alpha-ketoglutarate semialdehyde, which is subsequently oxidized in a nicotinamide adenine dinucleotide-dependent reaction to alpha-ketoglutaric acid. Since A. tumefaciens converts d-glucuronic acid to d-glucarate, a pathway from d-glucuronate to alpha-ketoglutarate in A. tumefaciens was determined.


Relationships between energy reserves and function in rat superior cervical ganglion.


Relationship between phospholipid biosynthesis and the efficiency of the arsenate transport system in yeasts. In studying the possibility that phosphoinositides which formed complexes with arsenic were involved in the arsenate transport system of yeasts, a comparative study of the phospholipid composition and metabolism was carried out both in Saccharomyces carslbergensis and in its arsenate-adapted variant, which showed a deficient inflow of arsenate. It was found that the lipid composition of the two organisms was quite similar, the main classes of phospholipids being phosphatidylcholine, phosphatidylethanolamine, and phosphoinositides. The only difference was a 1.5- to 2-fold increase in the proportion of inositides in the arsenate-adapted cells. When the transport of arsenate became inactivated in the nonadapted yeasts after a 30- to 60-min exposure to 10(-2)m arsenate, an increment of inositides of 29 to 50% over the original level was also detected. A study of the incorporation of radioactivity from uniformly labeled (14)C-maltose and from (32)P-orthophosphate ((32)P(i)) demonstrated a decreased rate of lipid biosynthesis in the arsenate-adapted cells as compared to the normal nonadapted ones. The turnover of the phosphate in phospholipids demonstrated no turnover in phosphatidylcholine and phosphatidylethanolamine, and a slow turnover in phosphoinositides. It could be inferred that a normal rate of phospholipid (phosphoinositides) biosynthesis is necessary to have a normal arsenate uptake and that inositide accumulation impairs both the mechanism responsible for the uptake and accumulation of arsenate and the rate of lipid biosynthesis. No differences were found in the deoxyribonucleic acid or protein content of the two types of cells. Also, the arsenate-adapted cells, once freed of external arsenate, showed an increased uptake of (32)P(i) from low external concentrations of phosphate (10(-6) to 10(-8)m, 10-fold over that observed in AsS cells). These results are indicative of independent behavior in phosphate and arsenate transport systems.


Kinetics of the biosynthesis of phospholipids in neurons and glial cells isolated from rat brain cortex.


Levels of metabolites and production of glucose in the lamprey brain.


Secondary tumors of the kidney.

Secondary tumors of the kidney.


Intravenous nalidixic acid in urogenital sepsis: report of 25 courses.


Lipid and fatty acid composition of brown adipose tissue in rabbit.


Chronic effects of trichloroethylene exposure.


Viral hepatitis: recent studies of etiology.


Tyzzer's disease syndrome in laboratory rats treated with adrenocorticotropic hormone.


The effect of phleomycin on poliovirus RNA replication.


Synthesis of the RNA of RNA-containing tumor viruses. I. The interval between synthesis and envelopment.


Guanidine inhibition of poliovirus: a dependence of viral RNA synthesis on the configuration of structural protein.


The structural proteins of Mengo virus variants.


Strain-specific antigen of the avian leukosis sarcoma virus group. I. Isolation and immunological characterization.


Synthesis of the virus-specific proteins of a conditionally lethal mutant of poliovirus under nonpermissive conditions.


Characterization of a temperature-sensitive mutant of polyoma virus.


Concentration and purification of vesicular stomatitis virus by polyethylene glycol "precipitation".


Dynamics of poliovirus-specific RNA synthesis and the effects of inhibitors of virus replication.


Protection of enteroviruses against heat inactivation by sulfhydryl-reducing substances.


Characterization of abnormal DNA formed in polyoma virus-infected cells.


Immune responses to H particles of poliovirus.


Partial purification of the Epstein-Barr virus and some properties of its DNA.


Arrhenoblastoma of the ovary. Presentation of a case report and a discussion of the differential diagnosis.


Effects of the intrauterine contraceptive device on endometrial enzyme and carbohydrate histochemistry. Endometrial biopsies at varying times in the menstrual cycle were obtained from 30 healthy women (16-41 years) to be used as controls, and 19 women (16-36 years), who wore an IUD (Mazlin spring, Lippes loop, or Birnburg bow) for from 5 months  to 4 years, in order to study histological changes in the endometrium in the presence of the IUD.  The biopsy specimen was divided into 2 parts, one of which was frozen, sectioned and stained for alkaline and acid phosphatases, employing Gomori's procedure.  The other specimen was fixed overnight, sectioned and stained for mucosubstances with the periodic acid-Schiff method, Alcian blue-periodic acid-Schiff method after diastase digestion, aldehyde fuchsin-Alcian blue sequence, and the high iron  diamine-Alcian blue sequence.  In addition sections were stained with hematoxylin and eosin for dating and notation of histologic changes.  The alkaline phosphatase and acid phosphatase enzymes showed definite cyclic activity in the control group and the presence of the IUD caused only minor alterations in the cycle.  The mucosubstances also showed a definite predictable cycle in the normal control group.  The study group with the IUD in place showed a similar cycle but the amount of acid mucosubstance was increased throughout all phases of the cycle.  In both groups the sulfomucins predominated during the preovulatory or proliferative phase and the carboxymucins predominated during the postovulatory or secretory phase.  Certain histologic changes rendered by the presence of the IUD were also evident in this study, 8 patients showing some evidence of mil d to moderate inflammation.  The findings support the popular theory that the IUD produces endometrial and intrauterine fluid changes that discourages implantation.  A discussion of this presentation is included.  It was suggested that a quantitative study of acid phosphatase levels would be relevant in understanding the action of the IUD.  Extensive investigation of the lysosomal system will supplement the reported data.  Another point was raised concerning the advisability of comparing effects of a variety of IUD designs and also the conclusion that failure of implantation is the cause of reduced fertility in the IUD wearer.


Rapid eye movement (REM) sleep in ophthalmic patients.


Effect of BCG on lymphatic lung clearance of dusts with different fibrogenicity. An experimental study on rats.


Melanocyte and Langerhans cell population of the cornea and limbus in the albino animal.


Long-term changes in sleep patterns in men on the South Polar Plateau.


Short-term changes in sleep patterns on arrival at the South Polar Plateau.


Primary malignant and benign tumors of the duodenum. A study of 18 cases stressing the clinical and roentgenographic features.


Hyperpigmentation due solely to increased plasma beta-melanotropin.


Autonomic control of the peripheral vascular system.


Lactic dehydrogenase inhibitors in NAD.

Lactic dehydrogenase inhibitors in NAD.


Three hydrolysis methods for 17-ketosteroid sulfates compared by colorimetric and gas-liquid chromatographic analyses.


Transplantation of a pancreatic insulinoma into an insulin-resistant patient with juvenile diabetes.


The effect of sleep on electroencephalographic abnormalities at a distance from the lesion; an all-night study of 30 cases.


Influence of adrenocorticotropin, cortisone, aspirin, and phenylbutazone on the rate of exfoliation and the rate of renewal of gastric mucosal cells.


Correlation of clinical, parasitological, and serological data of individuals infected with Entamoeba histolytica.


Hygienic guide series. Hydrogen cyanide.


Gastric secretion in duodenal ulcer, with particular reference to the diagnosis of Zollinger-Ellison syndrome.


Experimental acute and chronic viral hepatitis. Effect of antithymocyte globulin.


An electron nuclear double resonance and electron spin resonance study of semiquinones related to vitamins K and E-1a.


Plasma renin activity and blood pressure before and after renal denervation.


A paramagnetic monomeric molybdenum(V)-cysteine complex as a model for molybdenum-enzyme interaction.


A geriatric day ward in an English hospital.


Action of a toxic glycolipid of Corynebacterium diphtheriae on mitochondrial structure and function. The toxicity for mice of the cord factor of Cornebacterium diphtheriae, trehalose-6,6'-dicorynomycolate, was studied. The diphtherial cord factor showed a delayed lethal toxicity for mice; the median lethal dose was 120 mug. Mouse liver mitochondria were disrupted in vivo under the toxic action of the diphtherial cord factor into fragments deficient in both respiration and phosphorylation. The site of metabolic defect in the mitochondrial electron transport system was located at the region prior to the level of cytochrome c in the livers of cord factor-intoxicated mice. These effects of the diphtherial cord factor on the host-cell mitochondria were essentially similar to those of the cord factor of Mycobacterium tuberculosis, trehalose-6,6'-dimycolate.


Purificationa and properties of a fructose-1,6-diphosphate-activated lactate dehydrogenase from Streptococcus faecalis. An l-(+)-lactate dehydrogenase was purified approximately 35-fold from crude extracts of Streptococcus faecalis. The purified enzyme had an absolute and specific requirement for fructose-1,6-diphosphate (FDP) for catalytic activity. The concentration of FDP required for 50% maximal activity was about 0.045 mm. The activator was bound to the enzyme more effectively at pH 5.8 than it was at a neutral or alkaline pH. Activation appeared to involve a conformational change in the enzyme which made the substrate and coenzyme sites more accessible to the respective reactants. Among the evidence supporting this hypothesis was the fact that FDP lowered significantly the apparent K(m) for both pyruvate and reduced nicotinamide adenine dinucleotide. Moreover, the enzyme, which was quite heat stable in the absence of any of the reactants, was rendered heat labile by FDP.


Correlation of bacteria lipid composition with antibiotic resistance. The correlation of bacterial lipid composition with antibiotic resistance was investigated with particular emphasis on those organisms in which resistance may be related to membrane or envelope structure or function, as in resistance to tetracyclines and polymyxin. Chloroform-methanol-extractable lipids, phosphatidyl ethanolamine fractions, and both fatty acids of these lipid fractions and total fatty acids were studied by using thin-layer chromatography, gas chromatography, and infrared spectroscopy. Consistent quantitative differences were found between the fatty acid compositions of sensitive and resistant strains. Most notable was the fact that, in gram-negative organisms, resistant strains showed decreases in cyclopropane acids as compared with sensitive strains. These changes were found to be inherent in the strains and not due to growth stage or culture age. No significant qualitative differences were noted. In contrast, no such variation in fatty acid content was observed in penicillin-sensitive and resistant strains of gram-positive cocci. As significant alterations of fatty acid composition were noted in gram-negative strains resistant to antibiotics, we suggest that resistance is correlated to membrane or envelope lipid composition.


D-apiose reductase from Aerobacter aerogenes. A strain of Aerobacter aerogenes PRL-R3 has been isolated which utilizes d-apiose as its sole source of carbon. A new enzyme, d-apiose reductase, was discovered in this strain. The enzyme was not present when the strain was grown on d-glucose. d-Apiose reductase catalyzes the nicotinamide adenine dinucleotide-dependent interconversion of d-apiose and d-apiitol. The enzyme is specific for d-apiose and d-apiitol, with a few possible exceptions. The K(m) for d-apiose is 0.02 m. The K(m) for d-apiitol is 0.01 m. The enzyme is almost completely specific for the reduced and oxidized forms of nicotinamide adenine dinucleotide. When cell-free extracts were centrifuged at 100,000 x g for 1 hr, the enzyme remained in solution. Optimal activity for the reduction of d-apiose was obtained at pH 7.5 in glycylglycine buffer, whereas for the oxidation of d-apiitol it was obtained at pH 10.5 in glycine buffer. Enzymatic reduction of d-apiose was not appreciably affected by the presence of 0.02 m ethylenediaminetetraacetate. Paper chromatography and specific spray reagents were used to identify d-apiitol and d-apiose as the products of this reversible reaction. d-Apiose and d-apiitol did not serve as substrates for ribitol dehydrogenase and d-arabitol dehydrogenase from A. aerogenes PRL-R3.


The possible mediation by cyclic AMP of parathyroid hormone-induced stimulation of mitotic activity and deoxyribonucleic acid synthesis in rat thymic lymphocytes.


Behavior of virus in malignant degeneration of skin lesion in epidermodysplasia verruciformis.


Sarcomas of the calvaria; surgical experience with 14 patients.


Comparison of transformation and T antigen induction in human cell lines. Skin fibroblast cultures were established from eight individuals. These cell cultures, together with WI-38 cells, were examined for susceptibility to transformation by SV40 virus. Four transformation-susceptible cell lines (TS), established from patients with Down's syndrome, were found to be three to four times more susceptible to transformation than transformation-resistant cell lines (TR) from normal individuals. TR and TS cell lines were compared for their susceptibility to induction of SV40 T antigen. For dividing cells T antigen was detected in a higher percentage of TS cells than TR cells. For nondividing cells, the reverse was found; T antigen was detected in 10-fold more cells of the TR lines than in cells of the TS lines. Similar results were obtained after infection of cells with CELO virus. Titration of vaccinia virus and influenza virus A2/Scotland/49/57 indicated that TR and TS cells were equally sensitive to the former virus, but TR cells were three to five times more sensitive to influenza virus A2/Scotland/49/57 than were TS cells.


Induction of simian virus 40 antigen in BSC1 transformed cells. Heating to 45 C induced in virus-free clones of simian virus 40 (SV40) transformed BSC(1) cells the synthesis of SV40 viral antigen, as evidenced by immunofluorescence. Up to 3.8% of the cells exhibited viral antigen 72 hr after heating to 45 C for 30 min. Depletion of arginine from the medium of the heated cells enhanced and increased the percentage of cells synthesizing viral antigen to 11%. Cytosine arabinoside completely inhibited the induction of the viral antigen. No infectious virus was recovered from the cells in which synthesis of viral antigen was induced. However, small amounts of infectious SV40 virus were rescued from the BSC(1) transformed cells by fusion with rabbit kidney cells or by treatment with mitomycin C.


Antiviral activity of chlorite-oxidized oxyamylose, a polyacetal carboxylic acid. Intraperitoneal injection of chlorite-oxidized oxyamylose (COAM) protected mice against mengo, vaccinia, Semliki Forest, and influenza APR8 viruses. Topical administration in the eye of rabbits partially inhibited the development of experimental herpetic keratoconjunctivitis. COAM resembled polyacrylic acid in many aspects, but it was markedly less toxic. For systemic administration, the therapeutic index was on the order of magnitude of 1:300 to 1:500. Although the in vivo antiviral effect of COAM wore off faster than that of polyacrylic acid, protection lasted for several weeks. Against mengovirus, such prolonged protection was achieved only when polymer and virus were injected intraperitoneally. Protection against intravenous vaccinia virus was not dependent on the injection route of COAM. Experiments on the mode of action of COAM pointed to macrophages as possible mediators of the antiviral effect. The fact that small amounts of interferon appeared in the serum after administration of high doses of COAM suggests that interferon may play a role in the induction of antiviral resistance by COAM.


Polyacetal carboxylic acids: a new group of antiviral polyanions. Chlorite-oxidized oxypolysaccharides are polyacetal carboxylic acids. They inhibited the cytopathic effect of vesicular stomatitis virus in mouse embryo cell cultures challenged at low input multiplicity. After intraperitoneal injection of these compounds in mice, interferon appeared in the circulation. The compounds also protected mice against lethal mengovirus infection and against the development of experimental pox lesions on the tail. Chlorite-oxidized oxyamylose was antiviral only when at least 64% of the glucopyranose units were oxidized, an observation which suggested a correlation between charge density and antiviral effect. The antiviral activity was also influenced by the molecular weight, as demonstrated by the fact that chlorite-oxidized dextrans which had a high intrinsic viscosity were more active than those with low intrinsic viscosity.


Uncoating of poliovirus by isolated plasma membranes. Poliovirus type I LSc strain labeled with (14)C-uridine was adsorbed onto isolated plasma membranes and incubated with them. When membranes from Hep-2 or Vero cells were used, 22% of the label was converted to a trichloroacetic acid-soluble form, when trypsin or ribonuclease was added, the fraction rendered soluble was increased, and when the two enzymes were added in sequence, 85% or more of the label became trichloroacetic acid-soluble. This labilization of poliovirus could be reproduced when butanol-solubilized proteins from membranes were substituted for the whole plasma membranes, but it did not occur with membranes from polio-virus-resistant calf kidney or BHK-21 cells.


Synthesis and cleavage of enterovirus polypeptides in mammalian cells. Evidence is presented that the entire enterovirus ribonucleic acid genome is translated into a single giant polypeptide of well over 200,000 daltons molecular weight. This giant protein was detected repeatedly in coxsackievirus B1-infected cells, even in the absence of amino acid analogues. The enzymes which cleave this giant protein into smaller structural and enzymatic proteins accumulate with increasing time after infection. It appears that they are either virus-coded proteolytic enzymes or else host enzymes which are activated or released from an intracellular site as a result of virus infection. These cleavage enzymes do not cause grossly apparent cleavage of host-cell proteins.


Chemical studies on polyoma and Shope papilloma viruses. Polyoma and Shope papilloma viruses were purified and analyzed by chemical and physical methods. Disc electrophoresis of degraded virions indicated the presence, in both cases, of only one major species of polypeptide subunit. The weight of the peptide chain of polyoma virus was estimated in 8 m urea to be about 45,000 avograms, based on the sedimentation rate in a sucrose-urea gradient and the diffusion coefficient estimated from the differential migration in electrophoresis in gels of different pore size. The presence of a minor peptide of smaller size was suggested by carboxyl-terminal and sedimentation analyses. The amino acid composition of polyoma capsid protein was reported. Chemical analyses showed that polyoma virus and Shope papilloma virus contained 16 and 17.5% deoxyribonucleic acid, respectively. Light scattering by the polyoma virion showed it to have a molecular weight of 22 x 10(6) and a diameter of 54 nm.


Trypsinized placental cell cultures for the propagation of viruses and as "feeder layers". The preparation and properties of cell cultures derived from human placental tissue are described and their usefulness for the propagation of animal viruses and as "feeder layers" is indicated.


Irradiation of dihydrothymine in frozen sulfuric acid solutions.


Effect of angiotensin on myocardial function.


Effect of prolonged infusion of angiotensin II on ionic composition of the arterial wall.


Intranasal inoculation of feedlot calves with TELC strain parainfluenza-3 vaccine.


Contribution of venous resistance to total vascular resistance in skeletal muscle.


Thyroid hormone in heat-acclimated hamsters.


Effect of ethyl p-chlorophenoxyisobutyrate on rat adipose lipoprotein lipase activity.


Circadian rhythm of mouse liver phosphoenolpyruvate carboxykinase.


Angiotensin receptors.

Angiotensin receptors.


Litigation following vaccination of dogs.


Effects of prostaglandins on basal and stimulated thyroid function.


Carbamyl phosphate: glucose phosphotransferase activity of hepatic microsomal glucose 6-phosphatase at physiological pH.


5-Amino-4-hydroxyvaleric acid: a new intermediate in 5-aminolevulinate metabolism of Rhodospirillum rubrum.


Oxygen toxicity and carbon deprivation in Astasia longa.


On the specificity of cytidine diphosphate diglycerides in monophosphoinositide biosynthesis by rat brain preparations.


Carnitine palmitolyltransferase activity and fatty acid oxidation by livers from fetal and neonatal rats.


The role of magnesium ion in the adenine phosphoribosyltransferase reaction.


Studies of the binding of adenine to adenine phosphoribosyltransferase.


Influence of UV-inactivated HVJ on RSV rescue in cultures of different virogenic mammalian cells mixed with normal sensitive chicken cells.


A scanning, recording and integrating microinterferometer.


Discharge planning.

Discharge planning.


Action of cyclic adenosine 3',5'-monophosphate in vitro on the uptake and incorporation of uridine into ribonucleic acid in ovariectomized rat uterus.


The relationship of protein synthesis to early transcriptive events in bacteriophage T4-infected Escherichia coli B.


Analysis of the amino acid binding to the proline transfer ribonucleic acid synthetase of Escherichia coli.


Enzymes that destroy blood group specificity. I. Purification and properties of alpha-L-fucosidase from Clostridium perfringens.


The stimulatory effect of luteinizing hormone on adenyl cyclase in the bovine corpus luteum.


Purification and properties of ribonucleotide reductase from leukemic mouse spleen.


Gluconeogenesis in the isolated perfused rat kidney.


Inhibition of gluconeogenesis by butylmalonate in perfused rat liver.


The mechanism of action of ethanolamine deaminase. VI. Ethylene glycol, a quasi-substrate for ethanolamine deaminase.


Enhancement of the activity of horse liver alcohol dehydrogenase by modification of amino groups at the active sites.


Properties of purified methylmalonate semialdehyde dehydrogenase of Pseudomonas aeruginosa.


Requirement for reduced diphosphopyridine nucleotide-cytochrome b5 reductase in stearly coenzyme A desaturation.


Inhibition of the stearyl coenzyme A desaturase system by sterculate.


[Progress and limitation in x-ray diagnosis of stomach cancer].


Antibody responses to the Epstein-Barr virus and cytomegaloviruses after open-heart and other surgery.


Inapparent congenital cytomegalovirus infection. Clinical and epidemiologic characteristics in early infancy.


Bradykinin induced eosinophilia in "skin windows".


Bilateral Wilm's tumor: a case report and a survey of nineteen cases in Japanese literature.


A histopathologic study on the effect of alcohol on cirrhosis and hepatoma of autopsy cases in Japan.


Sleep patterns following 205 hours of sleep deprivation.


Topical antibiotic prophylaxis in contaminated wounds. Experimental evaluation.


Alkylated pyrimidine derivatives as antiviral agents. I. Syntheses and antiviral screening of alkylpyrimidine and 5-alkyluracil nucleoside.


Alkylated pyrimidine derivatives as antiviral agents. II. Inhibition of early stages of viral multiplication by 5-alkyluracil and 5-alkyluracil nucleoside.


The effects of DBM on rat ascites tumors and antibody production.


A study of ACTH and CRF in chickens.

A study of ACTH and CRF in chickens.


Lipids in vitamin A deficient chicken cartilage and new bone.


Phosphatase activity of listeria (preliminary report).


The role of vasotocin and of CRF in ACTH release in the chicken.


Course of air-borne infection caused by B virus (Herpesvirus simiae). 5. Significance of viraemia and role of blood leucocytes of B-virus, particularly air-borne infection of rabbits.


Study of pregnancy ketosis in the rat.

Study of pregnancy ketosis in the rat.


Preservation of the cornea for penetrating keratoplasty. An experimental study.


Vestibular influences during sleep. VI. Vestibular control of autonomic functions during the rapid eye movements of desynchronized sleep.


Modulation of sensory projections in anterolateral column of cat spinal cord by peripheral afferents of different size.


A comparison of sleep patterns in natural and mandrax- and tuinal-induced sleep. A within-subject comparison of the effects on the overnight sleep EEG of 1 tablet of Mandrax (containing methaqualone base 250 mg. and diphenhydramine hydrochloride 25 mg.) and 200 mg. Tuinal (equal parts of quinalbarbitone sodium and amylobarbitone sodium) in 14 normal subjects is reported.Mandrax-induced sleep was not significantly different from natural sleep in the duration of light, moderate, deep and REM phases. Tuinal produced a significant reduction in REM sleep (P < 0.01) compared with natural sleep and with Mandrax-induced sleep.


Effect of different levels of pulse protein (Cicer arietinum) on liver enzymes.


Inhibition of glyceraldehyde-3-phosphate dehydrogenase by glyoxylate.


The effects of diphosphonate, pyrophosphate and sodium foride on drinking habits of Osborne-Mendel rats.


Effects of pyrophosphate and diphosphonate on fluoride uptake by hydroxyapatite.


A FEW ENZYMATIC AND A/Nordio S, Cordone G, Gatti R, Marchi AG, Moscatelli P, Vignola P: A few enzymatic and immunologic researches in children with coeliac disease and other chronic enteropathies and with immunologic deficiencies.


Quantitative enzyme patterns in the nephron of the healthy human kidney.


Subacute toxicity of sterigmatocystin to rats.


Epidemiology of primary central nervous system neoplasms. II. Case-control study.


Enteroviruses in rural families and their domestic animals.


Benign nonchromaffin paraganglioma of the duodenum. Report of a case and review of the literature.


Liver enzymes in hypertension.

Liver enzymes in hypertension.


Cushing's syndrome secondary to a benign paraganglioma.


Measurement of acetyl coenzyme A in rat liver.


Studies on the chain length determination of large oligomers from MS2 RNA.


Interference by carbohydrate and other substances in the estimation of protein with the Folin-Ciocalteu reagent.


The quantitative and qualitative analysis of alkyl alpha-glycerol ethers as alkoxy acetaldehydes.


Reaction of inorganic phosphate with mitochondrial respiratory chain.


Vitamin D stimulation of calcium-dependent adenosine triphosphatase in chick intestinal brush borders.


Synthesis of estriol 16-alpha-(alpha-D-glucoside) by enzymic transglucosylation.


Acylglucoses of the corynebacteria and mycobacteria.


Interference of ethanol and sorbitol with hepatic ketone body metabolism in normal, hyper- and hypothyroid rats.


Effect of ethanol metabolism on redox state of steroid sulphates in man.


Energy production in rat parotid gland. Relation tonzyme secretion and effects of caium.


Regulation of liver pyruvate kinase and the phosphoenolpyruvate crossroads.


Amniographic diagnosis of trophoblastic disease.


The dissimilation of higher dicarboxylic acids by Pseudomonas fluorscens.


Isolation of anaerobic bacteria from clinical specimens.


Synchronization and coupling of phases in periodic processes of conditioning.


Histologic and lipid studies of sural nerves in inherited hypertrophic neuropathy: preliminary report of a lipid abnormality in nerve and liver in Dejerine-Sottas disease.


Effect of glucosamine and benzylidene-glucosamine on 35SO4-incorporation into cartilage and aortic tissues.


Sebaceous lymphadenoma of the parotid gland: associated with a case of bilateral parotid gland tumors.


Effect of mercurials on NADH oxidation and energy-linked hydrogen ion gradient in submitochondrial particles.


Angiographic localization of insulinomas: high reported success rate and two additional cases.


Some aspects of the binding of Cu (II) to human hemoglobin and its subunits.


Phosphorylation coupled to NADH oxidation with fumarate in Streptococcus faecalis 10Cl.


Preparation and properties of a cyclic 3',5'-nucleotide phosphodiesterase isolated from frog erythrocytes.


A neuromuscular syndrome of scapuloperoneal distribution.


Hereditary hypertrophic neuropathy. Report of two cases of an autosomal recessive variant.


The isolation of herpes virus from human corneal lesions.


A theoretical examination of ionic interactions between neural and non-neural membranes. Evidence from electron microscopy indicates that the separation between adjacent membranes of the central nervous system (CNS) is less than 500 A and perhaps as small as 100-250 A. The rapid K(+) efflux associated with the neural action potential may therefore be sufficient to affect the local extracellular potassium concentration and, via their partial dependence upon the potassium equilibrium potential, alter the electrical states of nearby neural and glial membranes. This new concept of a transient and local depolarizing "ionic interaction" between active and inactive membranes of the CNS is here examined theoretically and its magnitude calculated as a function of (a) the intermembrane separation, (b) the membranes' electrochemical characteristics, and (c) the rate at which K(+) can diffuse away from the vicinity of the active (neural) membrane. My results indicate that the interaction is in the millivolt range and therefore significant in the modulation of postsynaptic and presynaptic information processing; in particular configurations the postulated interaction alone may be suprathreshold. Membrane noise and local synchrony in groups of neurons may reflect these local, K(+)-mediated interactions. The transient ionic interaction between active neural and nearby glial membrane is also in the millivolt range; however, the relevance of neuroglia to neuronal function is obscure. Certain pathological states, such as seizure and spreading depression, have an obvious phenomenological correspondence to the results presented here and are briefly discussed.


Responses of neurons in the dorsal nucleus of the lateral lemniscus of cat to binaural tonal stimulation.


Corticofugal influence on activity of lateral geniculate neurons in the cat.


Rat liver plasma membranes in essential fatty acid deficiency.


Management of the narcotic-addicted surgical patient: concepts of medical and surgical care.


Wound healing: biochemical events and potential role of collagenase.


Observations on ultrastructural morphology of bronchogenic carcinoma.


Bronchial adenoma.

Bronchial adenoma.


Emulsified ethiodol as a bronchographic contrast agent. Preliminary observations.


Early appearance of the secondary vertebral ossification centers.


Skeletal manifestations of congenital cytomegalic inclusion disease.


Further studies in lymphokinetic considerations of lymphography and intralymphatic therapy.


The electron microscopic demonstration of acetylcholinesterase activity in some cholinergic and non-cholinergic synapses of the rat brain.


Rapid spectrophotometric method for determination of micro amounts of copper in proteins.


Micro method for the quantitative determination of delta 22-sterols.


Renal function, renin activity and endogenous vasoactive substances in cirrhosis.


Scintillation counting of 32P without added scintillator in aqueous solutions and organic solvents and on dry chromatographic media.


Method of determining oxygen concentrations in biological media, suitable for calibration of the oxygen electrode.


Automated multiple enzyme analysis systems.


Effects of adenosine 3',5'-cyclic monophosphoric acid on certain light-induced reactions and on ATPase activity of isolated chromatophores from Rhodospirillum rubrum.


Mössbauer spectroscopy of lyophilized rat liver microsomes.


Exchange of adenine nucleotides in digitonin-treated mitochondria.


The state of copper in stellacyanin and laccase from the lacquer tree Rhus vernicifera.


Non-heme iron (iron-sulfur) proteins of Azotobacter vinelandii.


Iron metabolism and absorption studies in the X-linked anaemia of mice.


Studies on the interaction between serum bactericidal activity and antibiotics in vitro.


The effect of adrenocorticotropic hormone and cortisone on the mucous cells of the rat stomach. I. Determination of hexosamines in the fundus and pyloric mucous membrane.


The effect of corticotrophine and cortisone on the content of fucose in stomach mucosa of white rats.


Proteins released from chick embryo fibroblasts in culture. II. Studies on cells in different states of culture.


Effects of various photoperiods on the temporal distribution of paradoxical sleep in rats.


Studies on succinate oxidation. I. The use of intact tissue sections.


Effect of procaine on asphyxial rigidity.


Stereospecificity of the prostaglandin 15-dehydrogenase from swine lung.


Microbiological assay of cyclic 3',5'-AMP.


Isolation of an antibiotic-resistant, lactose-fermenting strain of Proteus rettgeri.


Increase in antiviral activity of polynucleotides by thermal activation.


Primate electrophysiology, particularly related to sleep. ARL-TR-69-5.


The first isolation of Myxovirus influenzae A2-equi in Yugoslavia.


"Meningioma blush" in pituitary adenoma.


Accuracy of brain scanning. Comparison with other procedures for brain tumor detection.


Acinar-islet cells in the exocri pancreas of the adult cat.


Acute reversal of the sleep-waking cycle in man. Effect on sleep stage patterns.


Transient neonatal myasthenia. Clinical and electromyographic studies.


Steroidogenesis by the rabbit adrenal gland in vitro.


The antigenic component common to all Clostridium perfringens type A strains.


The NAD kinases of Saccharomyces cerevisiae.


The nature of inhibition of mitochondrial malate dehydrogenase by thyroxine, iodine cyanide and molecular iodine.


The phosphodiesterases in the rat pancreas.


Preliminary report on the effects of foot and mouth vaccination on semen quality of cross bred bulls. I.


Allergy and infection. IX. The significance of susceptibility factors.


Hereditary ulnar and fibular dimelia with peculiar facies. A case report.


Control by prostaglandin E2 of sympathetic neurotrans-mission in the spleen.


Relationship between sympathetic ganglion activity and audiogenic seizures in mice.


Three dimensional reconstructions of spherical viruses by fourier synthesis from electron micrographs.


Cooperative study in the evaluation of therapy in multiple sclerosis. ACTH vs. placebo--final report.


The relationship of nocturnal headaches to sleep stage patterns.


Long chain fatty alcohols in normal and neoplastic tissues.


Gold neuropathy.

Gold neuropathy.


Unusual Sphaerophorus species from the large intestine of man. An obligately anaerobic, gram-negative microorganism identified as a Sphaerophorus species was recovered from the fecal material of two cancer (chronic myelogenous leukemia and idiopathic thrombocythemia) patients receiving cobalt radiation therapy. The organism, isolated on sheep blood-agar, exhibited extreme pleomorphism (rods, filaments, and spheroids) and was a major component of the anaerobic fecal microflora. In one patient the numbers of Sphaerophorus species (designated as isolate 6-13-68), Bacteroides species, and Clostridium perfringens declined after irradiation; however, they were stable in this same patient after a second therapeutic dose of radiation. The numbers of anaerobes in the other patient remained fairly consistent after radiation. The biochemical and morphological characteristics and carbohydrate fermentation reactions of isolate 6-13-68 most closely resembled those of Sphaerophorus ridiculosis.


Comparison of media for direct isolation and transport of Shigellae from Fecal specimens. Xylose-lysine-deoxycholate (XLD) agar, SS agar, and MacConkey agar for isolating shigellae from fecal specimens were compared. XLD agar was superior to both SS agar and MacConkey agar for isolating Shigella sonnei, and both XLD and SS agar were superior to MacConkey agar for isolating S. flexneri. Direct plating of the fecal specimens in the field resulted in a greater yield of shigellae as compared to transporting specimens to the laboratory either in holding media or enrichment broth. Buffered glycerol saline was superior to other transport media evaluated, yielding 83% of shigella isolates when plated within 48 hr as compared to direct plating. The combination of XLD agar and SS agar is recommended for direct isolation of shigellae, and, whenever possible, these solid media should be taken to the bedside and inoculated directly.


Dehydrogenase patterns in the study of Bacteroidaceae. Enzyme patterns were obtained by starch-gel electrophoresis of cell-free extracts from organisms in the family Bacteroidaceae. Esterases, phosphatases, and lactic and succinic dehydrogenases were detected but offered no possibility for classification purposes. Alpha-glycerophosphate dehydrogenase and 6-phosphogluconate dehydrogenase were not detected. Zymograms of malic, glutamic, and glucose-6-phosphate dehydrogenases separated Bacteroides species from Sphaerophorus and Fusobacterium species. Malic dehydrogenase and glucose-6-phosphate dehydrogenase were found in Bacteroides but not Sphaerophorus and Fusobacterium. These dehydrogenase zymograms placed three gram-negative, nonsporeforming anaerobic rod isolates with the Bacteroides species. A close correlation was found between the classification of Bacteroidaceae by zymogram analysis and a numerical taxonomy scheme previously published from this laboratory.


Evaluation of a new incubation-type indole strip test. A total of 189 of Enterobacteriaceae and three strains of Clostridium perfringens were tested with a new incubation-type indole paper strip. This 4-hr incubation test gave the same results as a standard 48-hr indole test, except with some strains of Proteus rettgeri. Results with P. rettgeri varied somewhat according to the medium upon which the organisms had been grown.