Carbon balance of a mannitol fermentation and the biosynthetic pathway. The carbon balance was determined for a fermentation in which mannitol is produced from glucose by an Aspergillus species. The products found were: cells (17% of carbon input), CO(2) (26%), mannitol (35%), glycerol (10%), erythritol (2.5%), glycogen (1%), and unidentified compounds (8%). Thus, 92% of the carbon input was accounted for. Cell-free enzyme studies showed that mannitol was synthesized via the reduction of fructose-6-phosphate and not by the direct reduction of fructose. If the cell yield from glucose was assumed to be 50% and the theoretical conversion efficiency from glucose to polyols was 90%, as calculated from the energy balance, then 34% of the glucose carbon was used for growth and 53% was used for polyol formation.


The effect of dietary protein on carcinogenesis of aflatoxin.


Fatty inclusions and microcysts. Thioacetamide induced fatty inclusions in nuclei of mouse liver cells and hepatoma cells.


Tachyphylaxis to alpha- and beta-angiotensin in dogs perfused with Ringer's solution or blood.


A turbidimetric method for the determination of pentachlorophenol in urine.


Odontogenic tumors. A survey of their manifestations in childhood.


A program for the rehabilitation of socially disabled psychiatric patients through retraining.


Carcinogenicity of organic extracts of atmospheric pollutants.


Contagious ecthyma in man. Report of two cases with laboratory studies.


The role of the ovarian stroma in hormone production by ovarian tumours.


The response of isolated sheep and human umbilical arteries to oxygen and drugs.


Effect of smoking and nicotine on adrenocortical secretion.


The value of neomycin in a neomycin-steroid cream.


Amino acid-activating enzymes in yellow lupin seeds, and purification of leucyl-sRNA synthetase.


Isolation and properties of soluble ribonucleic acid from yellow lupin seeds.


Secondary structure of poly-N(4),5-dimethylcytidylic acid and its copolymers with cytidylic acid.


Ribonucleic acid accompanying deoxyribonucleic acid in the course of extraction from rat brain.


Pathways of nicotinamide adenine dinucleotide biosynthesis in nicotinic acid or nicotinamide requiring microorganisms.


Allosteric activation of DPN-linked malic enzyme from Escherichia coli by aspartate.


Activation of adenyl cyclase in thyroid homogenates by thyroid-stimulating hormone.


A beta-hydroxydecanoyl-ACP dehydrase specific for saturated fatty acid biosynthesis in E. coli.


Branched-chain sphingosines from Tetrahymena pyriformis.


Polyribosome patterns in EMC-virus infected Krebs-2 cells and cell extracts.


Hydrolytic activity of muscle acyl phosphatase on 3-phosphoglyceryl phosphate.


Presynaptic inhibition in crustacean muscle: axo-axonal synapse.


Cholinergic actions related to paradoxical sleep induction in the mesencephalic cat.


Failure of cortisone and ACTH to regulate cardiac hypertrophy.


Structure activity relationships of compounds able to suppress the antipolio action of guanidine.


Effect of periodate on competence in Bacillus subtilis.


Translation and polarity in the histidine operon. 3. The isolation of prototrophic polar mutations.


Histidine regulatory mutants in Salmonella typhimurium. IV. A positive selection for polar histidine-requiring mutants from histidine operator constitutive mutants.


Translation and polarity in the histidine operon. II. Polarity in the histidine operon.


Experimental observations concerning the effects of mineral dust to pulmonary tissue.


Program evaluation and planning in a community mental health service.


Schizophrenic patients in 1950-1952 and 1957-1959. A comparative study.


A comparison of two populations within a psychiatric clinic.


Intensive treatment of the chronic schizophrenic.


The effects of hydrocortisone and ACTH on the gastric mucosa of the rat.


Pancreatic tumor with intractable watery diarrhea, hypokalemia and hypercalcemia electrolyte balance studies.


The duration and control of adrenal function in adenohypophysectomized cockerels.


Microangiopathic haemolytic anaemia associated with consumption coagulopathy in a patient with disseminated carcinoma.


Herpes encephalitis.

Herpes encephalitis.


Pathology of the kidney and liver in the experimental leptospirosis of the guinea-pig. A light and electron microscopy study.


Cushing's syndrome caused by bronchial adenomas.


Primary mediastinal tumors.

Primary mediastinal tumors.


Early evaluation of peripheral nerve lesions in continuity with a note on nerve recording.


Renin and angiotensin II studies in malignant hypertension after x-irradiation for seminoma.


Islet cell tumors of the pancreas.

Islet cell tumors of the pancreas.


Electron spin resonance of microsomal cytochromes. Correlation of the amount of CO-binding species with so-called microsomal e-x in microsomes of of Sudan III-treated animals.


The low-temperature spectral properties of mammalian cytochromes oxidase. II. The enzyme isolated from beef-heart mitochondria.


The action of amytal on frog gastric mucosa.


Cytochrome oxidase and its derivatives. 8. Formation and properties of the 'oxygenated' form of cytochrome oxidase and its reconversion to ferrous and ferric oxidase.


Testosterone and hydrocortisone-stimulated responses of reduced pyridine nucleotide fluorescence in prostates cultured from castrate rats.


Electron paramagnetic resonance studies on the cytochrome oxidase from yeast.


A simplified procedure for the preparation of cytochrome oxidase by non-ionic detergent.


Intracellular localisation of phosphoglycollate phosphatase and glyoxalate reductase.


Isolation of high-molecular-weight NADH dehydrogenase of the respiratory chain by diethyl ether--Triton X-100 treatment.


Chromatography of lipids. I. An improved chromatographic procedure for the quantitative isolation of the neutral ceramide-containing glycolipids from mammalian tissues.


Chromatography of lipids. II. The quantitative isolation of the minor (acidic) phospholipids and of phosphatidylethanolamine from the lipid extracts of mammalian tissues.


Palmitate and glucose metabolism in tumors induced by Rous sarcoma virus.


Phospholipases in arterial tissue. I. Sphingomyelin cholinephosphohydrolase activity in human, dog, guinea pig, rat and rabbit arteries.


Characteristics of rat liver phosphatidylinositol kinase and its presence in the plasma membrane.


The phospholipid compositions of some Actinomycetes.


Monophosphatidyl inositol inositolphosphohydrolase in guinea-pig brain.


The unsaturated fatty acids of Escherichia coli.


Mammary tumor virus in a spontaneous transplantable male mammary tumor.


The effect of temperature on the formation of delta 5-unsaturated fatty acids by bacilli.


Phagocytosis and degradation of elastin by normal and tumor cells in culture.


Immunology of the mouse mammary tumor virus (MTV): neutralization of MTV by mouse antiserum.


Studies on respiration and glycolysis in transplanted hepatic tumors of the rat.


DNA metabolism in liver and kidney tumors of different growth rates.


Defective dietary control of fatty acid metabolism in four transplantable rat hepatomas: numbers 5123C, 7793, 7795, and 7800.


Physiological effects of corticosteroids on the eye.


Effects of pharmacological agents on the physiological responses of hair discs.


Pharmacological effects of corticosteroids.


Steroid therapy in uveitis.

Steroid therapy in uveitis.


Use of corticosteroids in neuroophthalmology.


Experimental enteritis with food poisoning and classical strains of Clostridium perfringens type A in lambs.


Cortisone glaucoma.

Cortisone glaucoma.


Studies on the interaction between endotoxin and polymyxin B.


Plasma enzyme changes in Listeria monocytogenes-infected mice.


Intracranial extensions of juvenile angiofibroma of the nasopharynx.


Antigens of epithelial basement membranes of mouse, rat, and man. A study utilizing enzyme-labeled antibody.


Influence of adrenocorticotrophic hormone (ACTH) on ovum-implantation induced by estradiol and progesterone in ovariectomized rats.


Pathological studies on bone marrow in equine infectious anemia. II. Histopathology of vertebral, sternal and femoral bone marrow.


Equine infectious anemia occurring in Hokkaido, Japan--its histopathology and a critical view of the occurrence and diagnosis of this disease.


Virus-like particles in human oral papilloma.


Hypocalcemia and tetany with steroid-induced acute pancreatitis.


Hepatic abscess: review of seven patients seen at Wilford Hall USAF Hospital, 1956-1966.


Allosteric properties of the Mg++-dependent inorganic pyrophosphatase in mouse liver cytoplasm.


Basicity, visible spectra, and electron spin resonance of flavosemiquinone anions.


Magnetic and spectrophotometric investigations of cytochrome b5.


Effect of lysine vasopressin dimer on corticotropin release in man.


Coexistence of primary ovarian insufficiency, primary adrenocortical insufficiency and idiopathic hypoparathyroidism.


The regional and subcellular distribution of 2',3'-cyclic nucleotide 3'-phosphohydrolase in the central nervous system.


Alterations in the activities of several rat liver enzymes at various times after initiation of a high protein regimen.


A&UTORADIOGRAPHIC STUDIES ON DNA biosynthesis in Entamoeba histolytica in CLG medium.


Changes associated with parasitism in nematodes. II. Histochemical and microspectrophotometric analyses of preparasitic and parasitic larvae of Meloidogyne javanica.


A simple chromatographic method for the estimation of small amounts of 17,21-dihydroxy-20-ketosteroids in urine.


Properties of steroidal 3 enol heptafluorobutyrates.


The products of methylation of the four estriol epimers by dimethyl sulfate.


Microphysiology of vasopressors in shock.


Arrhenoblastoma. A case report.

Arrhenoblastoma. A case report.


Granular cell myoblastoma of the vulva.

Granular cell myoblastoma of the vulva.


Sedimentation coefficient of polyoma virus DNA.


Muscle type isoenzymes of liver aldolase in hepatomas.


Changes in DNA synthesis and cell proliferation during hepatocarcinogenesis by diethylnitrosamine.


Cytochemical studies of some cytoplasmic structures in embryo and newborn rat liver and in hepatoma.


Influence of cortisone and ACTH on the incorporation of S35 into the mucous cells of the intestine of albino rats.


Cystosarcoma phyllodes.

Cystosarcoma phyllodes.


A clinico-pathological study of bone tumours.


The suprarenal cortical function as measured by the plasma 17-hydroxycorticosteroid level in malnourished children. II. In kwashiorkor.


A comparison of two neutralization methods for typing enteroviruses.


The neurophysiological basis of motor learning. A review.


Cylindroma of the trachea.

Cylindroma of the trachea.


Susceptibility of Pseudomonas to colistin, kanamycin and polymyxin-B.


18F scanning of primary and metastatic bone tumours.


Differentiation between vibrio cholerae and Vibrio cholerae biotype El Tor by the polymyxin B disc test: comparative results with TCBS, Monsur's, Mueller-Hinton and nutrient agar media.


Ascarosides and ascaroside esters in Ascaris lumbricoides (Nematoda).


The transport of 14C-labelled material from CNS to and from muscle along a nerve trunk.


Phospholipase activity in Musca domestica L.


The influence of gonadal hormones and biotin deficiency upon liver lipid composition and liver fatty acid distribution in female chicks fed either a fat-free or fat-supplemented diet.


Induction of "avian leucosis group-specific" antigen synthesis in chick embryo fibroblasts by template active RNA from avian myeloblastosis virus.


Sporulation of mesophilic clostridia.

Sporulation of mesophilic clostridia.


Fundamental features of the "minimal deviation" hepatoma 5123.


Killed fowl plague virus vaccine obtained from chick embryo cells.


Cellular neurophysiological approaches in the study of learning.


Theory and practice of the rehabilitation of the psychiatrically disabled.


Effect of mechanoreceptor stimulations of different intensities originating from the stomach andlungs on the afferent impulsation of the cervical vagus.


Adrenergic neurone blocking agents of tropane and granatane structure. Their effect on the guinea pig vas deferens.


Aldosterone in childhood.

Aldosterone in childhood.


Clinical evaluation of an intradermal skin test for amebiasis.


Herpes virus in the lacrimal gland, conjunctiva and cornea of man--a chronic infection.


The dynamics of adrenocortical secretion.


Nursing referral outcome for post-hospitalized chronically ill patients.


Zollinger-Ellison syndrome in a patient with multiple carcinoid-islet cell tumors of the duodenum.


Studies on the structure of poliovirus.

Studies on the structure of poliovirus.


On the cpsid structure of some small feline and bovine RNA viruses.


Susceptibility of rabbit kidney cells of various passage levels to infection with SV-40 virus.


Determination of equine serum inhibitors for poliovirus by the gel-adsorption technique.


The study of vesicular stomatitis viral components by x-irradiation.


Posterior subcapsular cataracts and macular lesions after long-term corticotrophin therapy.


Corneal hypersensitivity to herpes simplex.


Epidemiological study of Clostridium perfringens (welchii) food poisoning--the carrier state and its variation in humans.


Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster.


Kinetics and dissociation constants of liver alcohol dehydrogenase with 3-acetyl pyridine NAD+ and NADH.


A study o DNA, partially denatured DNA and protein-DNA complexes in the polyethyleneglycol-dextran phase system.


A fraction of newly synthesized DNA of HeLa cells.


The role of phosphatidate phosphohydrolase in glyceride biosynthesis.


Enzymatic synthesis of DNA with 4-thio-thymidine triphosphate as substitute for dTTP.


Studies on phytosterol biosynthesis. The presence of  4-alpha,14-alpha-dimethyl-delta-8,24(28)-ergostadien-3-beta-ol in grapefruit peel and its co-occurrece with cycloeucalenol in higher plant tissues.


Significance of the so-called oval cell proliferation during azo-dye hepatocarcinogenesis.


Evidence for a fastr degradation of an atypical hydroxyproline and hydroxylysine deficient collagen formed under the effect of 2,2'-dipyridyl.


Histopathological study on early carcinoma of the stomach: some considerations on the ulcer-cancer by analysis of 144 foci of the superficial spreading carcinomas.


Inhibition of alpha- and beta-hydroxysteroid dehydrogenases and steroid delta-isomerase by substrate analogues.


Rhinoviruses: basis for a numbering system. 1. HeLa cells for propagationand serologic procedures.


Rhinoviruses: basis for a numbering system. II. Serologic characterization of prototype strains.


Studies of live attenuated Japanese encephalitis vaccine in swine.


The pathogenicity in mice of aerosols of encephalomyocarditis group viruses or their infectious nucleic acids.


Histogenesis of malignant liver tumors induced by dimethylnitrosamine. An experimental study in Syrian hamsters.


Study of S91 mouse melanomas by electron paramagnetic resonance (EPR) spectroscopy and tissue culture. II. Effects of penicillamine on EPR signals and on growth in vitro.


"Minimal deviation" hepatomas: a critical review of the terminology, including a commentary on the correlation of enzyme activity with growth rate of hepatomas.


Biophysical and bioassay studies on milk fractions of genetically similar agent-bearing agent-free mouse substrains.


Antibodies for Rous sarcoma virus (Bryan) in fowl, animal, and human populations of East Africa. II. Antibodies in domestic chickens, wildfowl, primates, and man in Kenya, and antibodies for Burkitt lymphoma cells in man.


Studies on acute leukemia and infectious mononucleosis of childhood. I. Viral interference with lymphoblastoid cells of spontaneously transformed bone marrow cultures.


Bone marrow colony-stimulating activity of serum from mice with viral-induced leukemia.


Discrete potentials in the dark-adapted ye of the crab Limulus.


Deficiency of erythrocyte nicotinamide adenine dinucleotide nucleosidase (NADase) activity in the Negro.


Influence of positive inotropic agents on the action of a myocardial depressant factor in the plasma of cats in postoligemic shock.


Effect of hyperthermia on blood platelets in male rats.


Ribonucleic acid synthesis and nuclear ribonucleic acid polymerase activity in livers of mice infected with mouse hepatitis virus (MHV-3).


Plaques of rhinoviruses in human diploid cells.


DNA biosynthesis in monkey kidney cells infected with PARA (SV40)-adenoviruses.


Effect of streptovitacin A on replication of an RNA virus (poliovirus).


Plaque assay for herpes simplex virus in L-929 (Earle) mouse fibroblasts.


Racial and prenatal factors in major congenital malformations.


Wilms' tumor and neurblastoma.

Wilms' tumor and neurblastoma.


Renal artery constriction: physiological determinants of pressure gradients.


Anomalous response of certain chicken embryo to various inocula. II. Evidence of a biological agent associated with resistance to rous sarcoma virus infection.


Pituitary and adrenal influence on reduced insulin B-chain induced hyperglycemia.


Insulin secretion by insuloma and normal pancreas slices. Studies in a patient with multiple endocrine adenomata.


Asparagine biosynthesis by the Novikoff Hepatoma isolation, purification, property, and mechanism studies of the enzyme system.


Citryl 1-phosphate and the citrate cleavage reaction.


Nephrotic syndrome and nephroblastoma. Report of a case.


Thermal conductivity of glycine.

Thermal conductivity of glycine.


Electron spin resonance and optical spectra of haemoglobin-M Hyde Park.


Activation of glucose-6-phosphatase of rat liver at high pH: differential response of basal and induced activities.


Virilizing lipoid cell tumor of the ovary. Report of 2 cases, 1 with uterine hypertrophy.


Theca cell tumor associated with pregnancy. Report of acase.


Meigs-like syndrome secondary to Krukenberg's tumor.


Immunopathological aspects in endocrine disorders (adrenals and pituitary gland).


Acute necrotizing encephalitis: observations of herpes-like particles by electron microscopy.


Effect of cortisone on nonfatal mouse cytomegalovirus infection.


Collagenolytic activity in the rabbit and sow Graafian follicle during ovulation.


Cytopathic effects of parainfluenza virus Type 3 in organ cultures of human respiratory tract tissue.


Effect of angiotensin amide on sodium isotope flux and short-circuit current of isolated frog skin.


Effect of aortic constriction on renin secretion in normal and hypophysectomized dogs.


A cyclic phosphodiesterase with 3'-nucleotidase activity from Proteus mirabilis.


Comparisons of the effects of Bacillus subtilis protease, type VIII (subtilopeptidase A), and insulin on isolated adipose cells. II. Antilipolytic action.


A silica-gel trap system for dehumidification of expired gases in a nonrebreathing anesthetic or respiratory circuit.


Effect of metyrapone on the excretion of oestrogen in man.


Emile Vidal (1825-1893), Parisian dermatologist.


Association of antigenic type of Herpesvirus hominis with site of viral recovery.


Murine hepatitis virus: effect on liver RNA. After infection of mice with hepatitis virus MHV3, the RNA in the liver undergoes changes. The fraction extracted with phenol at 0 degrees C does not alter. However, the fraction extracted with hot phenol at elevated pH (60 degrees C, pH 8.3) shows a 16S peak on sucrose-density-gradient centrifugation. This fraction shows actinomycin D-resistant incorporation of C(14)-orotic acid in infected but not in control livers-possible evidence of the RNA nature of MHV3.


Immunization of horses against African horse-sickness with attenuated neurotropic viral strains propagated in chicken embryos.


Assessment of pituitary-adrenal gland function in the dog.


Klinefelter's syndrome and cancer of the breast.


Eccrine glands of the rat. Response to induced sweating, hypertension, uremia, and alterations of sodium state.


Pathology of ovarian neoplasms in rats and mice.


Simultaneous fluorometric determination of cortisol and corticosterone in human plasma.


Hypoglycemia in a two-year-old boy with adrenocorticotropic hormone (ACTH) deficiency (probably isolated) and adrenal medullary unresponsiveness to insulin-induced hypoglycemia.


Congenital adrenal hyperplasia due to a deficiency of one of the enzymes involved in the biosynthesis of pregnenolone.


Gonadotropin-dependent Krukenberg tumor causing virilization during pregnancy.


Characterization and source of a renin-like enzyme in anephric humans.


Adrenocortical failure in panhypopituitarism.


Adrenal function in patients treated with testosterone and fluorinated pyrimidines.


Cariostatic effect of a stannous fluoride-containing dentifrice on children: two-year report of a supervised toothbrushing study.


Herpes simplex encephalitis.

Herpes simplex encephalitis.


Cranial nerve syndromes. Relation to exogenous and endogenous substances.


Outpatient treatment of a third degree burn.


Cardiac rehabilitation. 3. Follow-up after myocardial infarction.


The auriculo-temporal or Frey syndrome and tympanic neurectomy.


The renin-angiotensin system and hypertension.


Brotherly lumps. Familial occurrence of chemodectoma.


Ultrasonic selective hypophysectomy.

Ultrasonic selective hypophysectomy.


Cytolytic toxins of bacterial origin. The nature and properties of cytolytic proteins are discussed with emphasis on staphylococcal alpha-toxin.


Corticotropin release: inhibition by intrahypothalamic implantation of atropine. Anterior hypothalamic implantations of crystalline atropine markedly inhibit the adrenocortical responses evoked by surgical stress, ether anesthesia, or intravenolus injection of arginine vasopressin. Similar implants in nearby regions of the brain or sham implantations in the same region were ineffective. The data suggest that the hypothalamic control of pituitary corticotropin may have a cholinergic component.


Carcinogens 3,4-benzpyrene and 3-methylcholanthrene: induction of mitochondrial oxidative enzymes. Sections of liver from rats injected with 3,4-benzpyrene and 3-methylcholanthrene, when incubated in mediums specific for the histochemical demonstration of mitochondrial oxidative enzymes, show greater activity of several of these enzymes than do sections from control rats. This observation was confirmed by comparison of the staining of mitochondria isolated from the control and from "induced" rats. The fact that an inhibitor of protein synthesis, actinomycin D, effectively diminished the stimulation provided evidence that the stimulation of activity is due to an increase in enzyme synthesis, generally called induction.


Denervation atrophy in isoniazid polyneuropathy. Histo-enzymatic study.


Experimental stromal herpes simplex keratitis in rabbits.


Electron spin resonance studies on heart muscle succinic dehydrogenase under low partial pressures of oxygen.


An electron paramagnetic resonance study of nitric oxide hemoglobin derivatives.


Fluorofumarate--a substrate for fumarate hydratase.


Charge-transfer character of glucose dehydrogenase inhibition by substituted pyridines.


Hg2+-induced dissociation of exchange reactions from catalytic activity of 2,3-diphosphoglycerate-dependent phosphoglyceromutases.


The effect of angiotensin on renal carbonic anhydrase.


Are 5-hydroxytryptamine and noradrenaline inhibitory transmitters in the medial geniculate nucleus?


Prospects for radiation treatment of herpetic and other affections of the anterior segment of the eye.


Adsorbent as a variable in thin-layer chromatography of Elapidae venoms.


The effect of angiotensin on renal circulation during mannitol diuresis.


Effect on adrenal steroid secretion of changes in renal haemodynamics in the rat.


LTH production and steroid excretion during the  menstrual cycle.


Influence of phosphate compounds on certain fungi and their preservative effects on fresh cherry fruit (Prunus cerasus, L.). Studies were conducted to ascertain the retarding effects of four phosphate compounds (sodium hexametaphosphate, sodium tripolyphosphate, sodium tetraphosphate, and tetrasodium pyrophosphate) on molding of fresh cherries (Prunus cerasus, L.). In vitro studies on their antimycotic effects against the most common fungal spoilers, Penicillium expansum, Rhizopus nigricans, and Botrytis sp., were also carried out. Sodium tetraphosphate appeared to be the most effective compound in preserving cherries and also had the greatest antimycotic effects in the in vitro studies. A 10% concentration, when applied as a dip, inhibited fungal growth on fresh cherries for up to 30 days of storage at 1.1 C (34 F) and a relative humidity of 94%, whereas untreated controls showed fungal growth at 14 days. Following in order of effectiveness were sodium hexametaphosphate, sodium tripolyphosphate, and tetrasodium pyrophosphate.


Production of specific varicella antiserum.


Respiratory chain of antimycin A-producing Streptomyces antibioticus. An active respiratory chain system was demonstrated in sonically treated mycelium of Streptomyces antibioticus, the producer of antimycin A. The respiratory electron transfer from substrate to oxygen proceeded successively through flavoprotein(s), b-, c-, and a-type cytochromes, and terminated with the cyanide-sensitive cytochrome oxidase. The cytochrome composition of the culture was not affected by the age of the mycelium, the intensity of antimycin A production, or differences in the media. Slater factor, coenzyme Q, and vitamin K were not interposed as hydrogen carriers in the respiratory chain between flavoproteins and cytochromes. The oxidation of reduced nicotinamide adenine dinucleotide and succinate was unaffected by antimycin A. Evidence is presented in support of the absence of the antimycin A-sensitive site from the electron transport system of S. antibioticus.


Improved medium for sporulation of Clostridium perfringens. An improved sporulation medium has been developed in which all five strains of Clostridium perfringens tested exhibited a 100- to 10,000-fold increase in numbers of spores when compared with spore yields in SEC medium under comparable conditions. In addition, three of five strains produced a 100- to 1,000-fold increase, with the remaining two strains yielding approximately the same numbers of spores, when compared with strains cultured in Ellner medium. At the 40-hr sampling time, 18 of 27 strains produced a 10- to 100-fold increase in numbers of spores in our medium, when compared to spore production obtained in a medium recently reported by Kim et al. The new medium contained yeast extract, 0.4%; proteose peptone, 1.5%; soluble starch, 0.4%; sodium thioglycolate, 0.1%; and Na(2)HPO(4). 7H(2)O, 1.0%. In some cases, the spore yield could be increased by the addition of activated carbon to the new medium. The inclusion of activated carbon in the medium resulted in spores with slightly greater heat resistance than spores produced in the new medium without added carbon or in SEC or in Ellner medium. The major differences in heat resistance of the various strains appeared to be genetically determined rather than reflections of a particular sporulation medium. A definite heat-shock requirement was shown for four of four strains, with the optimal temperature ranging from 60 C for a heat-sensitive strain to 80 C for a heat-resistant strain. Heating for 20 min at the optimal temperature resulted in a 100-fold increase over the viable count obtained after heating for 20 min at 50 C.


A study of cortisol metabolism in patients with chronic asthma.


The lipids in fossils.

The lipids in fossils.


Gastric suphilis: a case with pernicious anemia.


Paradoxical increase of peripheral glucose utilization in a patient with insulinoma treated with prednisolone.


Hypothalamic centers regulating the secretion of adrenal corticoids.


Emergency liver resection for primary carcinoma.


Synchronous behavior pattern of key glycolytic enzymes: glucokinase, phosphofructokinase, and pyruvate kinase.


Plasma growth hormone responses and growth retardation of hypothyroidism.


The role of host factors in mouse trophoblastic tissue growth.


Granular cell myoblastoma of the clitoris.


Glycogen content of the cerebral cortex of hyperthermia and hypthermia generated by environmental conditions and administration of several drugs.


Carcinogenesis in different animal specids by diethylnitrosamine.


The chemical composition of the cytoplasmic membrane of Bacillus subtilis.


Cytochemical studies of polyoma virus infection. 3. Deoxyribonuclease II.


Cytochemical studies of polyoma virus infection. IV. Enzymatic digestion of nucleic acids.


Changes in the activity of intracellular enzymes in neurogenic muscle atrophy.


Histochemical studies of KB cell cultures infected with parainfluenza 3 virus. II. The activity of lysosomal and oxidative enzymes in virus-infected cells.


Double-minutes--a chromosomal aberration in Rous sarcomas in mice.


Chromosomal analysis of ninety-one primary Rous sarcomas in the mouse.


7-deazaadenine ribonucleosides. The use of periodate oxidation in degradation studies.


Adrenocortical influences on free-operant avoidance behavior. Rats were conditioned to avoid shock on a free-operant avoidance schedule in which no exteroceptive stimulus signaled impending shock. Injections of adrenocorticotropic hormone (ACTH) or dexamethasone raised blood levels of glucocorticoids. These increases were accompanied by changes in avoidance performance: there was a higher frequency of long-duration interresponse times, a greater stability among them, and fewer short interresponse times, total responses, and shocks.


A drug to beat viruses. The story of methisazone.


Transport of noradrenaline and dopamine-beta-hydroxylase in sympathetic nerves.


Myxoviruses and infections of the nervous system.


Additional data on measles virus antibody and antigen in subacute sclerosing panencephalitis.


Pathogenesis of viral infections of the nervous system.


The metabolism of 11-dehydrocorticosterone by frog adrenal glands.


Endolymphatic therapy for malignant melanoma.


Acute necrotizing encephalitis.

Acute necrotizing encephalitis.


Disease from monkeys.

Disease from monkeys.


Dysmenorrhoea.

Dysmenorrhoea.


Pathophysiology and therapy of the fasting hypoglycemias.


Studies on polypeptides. XXXIX. Elimination of the imidazole portion of histidine as an essential site for biological function of angiotensin.


Regulation of Staphylococcus aureus lactate dehydrogenase. The effect of growth conditions on the specific activity of nicotinamide adenine dinucleotide (NAD)-linked lactate dehydrogenase (LDH) in extracts of Staphylococcus aureus strain SG 511A was examined. Kinetic and electrophoretic experiments, with extracts prepared from aerobically and anaerobically grown cells, provided evidence for only one physiologically significant enzyme. The aerobic level of NAD-linked LDH of S. aureus remained constant and was independent of the carbon source. In contrast, the level of LDH produced in an anaerobic environment was variable and was dependent on the carbon source. Growth anaerobically on pyruvate, as the sole fermentable carbon source, resulted in a maximal level of LDH activity, a value about eightfold greater than the aerobic level. Anaerobic growth either on pyruvate plus glucose or on glucose alone, however, resulted in approximately a threefold decrease in this maximum. Experiments with a heme-requiring auxotroph derived from S. aureus demonstrated that the aerobic level of LDH activity was dependent on a functional respiratory chain.


Lactate dehydrogenase activity in certain strains of Staphylococcus aureus. Lactate dehydrogenase (LDH) was studied in phage-propagating strains 29, 3A, 6, 81, and 42D of Staphylococcus aureus selected from the five groups in the International-Blair series. Cells were cultivated in Brain Heart Infusion (Difco) under nearly anaerobic conditions and were harvested near the end of the log phase. LDH activity was maximal at the end of the exponential growth period and was measured spectrophotometrically by reduction of p-nitro-blue tetrazolium, with phenazine methosulfate as a coupling agent. Crude enzyme extracts were prepared both by an acetone extraction technique and by sonic treatment. LDH activity for these enzyme preparations was determined by the colorimetric method mentioned and also by measuring the rate of nicotinamide adenine dinucleotide reduction at 340 mmu. The order of activity observed, by use of both assay methods, was 29 > 81 > 6 > 3A > 42D. LDH forms (possibly isoenzymes) for each of 15 strains, which represent the five phage-propagating groups of the International-Blair series, were separated by acrylamide gel electrophoresis. Five forms were distinguished and arbitrarily numbered on the basis of their rate of migration, no. 5 being the slowest component. No one strain had more than four, nor fewer than two, LDH forms. Form 3 appeared in 13 of the 15 strains and was followed in frequency by no. 2, 1, 4, and 5.


Structure of viruses of the papilloma-polyoma type. IV. Analysis of tilting experiments in the electron microscope.


Effect of starvation on the in vivo metabolism and effect of drugs in female and male rats.


Case records of the Massachusetts General Hospital. Case 52-1967.


The antibiotic sensitivity of Bordetella in South Australia.


Adrenal cortical function as reflected by the plasma hydrocortisone and urinary 17-ketogenic steroids in relation to surgery in elderly patients.


Interference of phenoxybenzamine and guanethidine with the vasoconstrictor responses of noradrenaline and angiotensin II in the hand.


Studies on the metabolism of chlorprothixene (Truxal) in rats and dogs.


Quantitative analysis of sulfolipid (sulfoquinovosyl diglyceride) and galactolipids (monogalactosyl and digalactosyl diglycerides) in plant tissues.


A histological study of the effects of the herpesvirus of infectious bovine rhinotracheitis in the lactating bovine mammary gland. Mastitis was produced in three first calf heifers by inoculation of the udder with the virus of infectious bovine rhinotracheitis. These animals were killed at different times during the course of the disease and tissues were harvested and examined histologically. The basic lesion resulted from the necrotizing effect of the virus upon the epithelial cells of the alveoli and ducts. Eosinophilic intranuclear inclusion bodies were demonstrated in tissues from animals killed at three and six days postinoculation, but not at nine days. Of interest was the presence of a large Reed-Sternberglike cell in tissues of the last two animals killed.


Electron microscopic study on the ascites hepatoma--comparative observations.


Observation of changes in volume of a congested limb as a means of studying the behaviour of capacity vessels. 1. Experiments have been performed to determine whether the volume of a forearm congested by a cuff inflated to a low pressure (15-30 mm Hg) reflects active changes in the vascular capacity of the part while remaining relatively insensitive to the passive effects of changes in perfusion.2. Conditions were chosen in which existing evidence suggests that blood flow and capacity vary independently of each other.3. The results of these experiments suggested that active changes in capacity can be studied by the continuous observation of the volume of a congested limb, provided that the associated change in blood flow is a reduction from the basal level and is not an increase above the basal level.


Cyclic thrombocytopenia of acute type.

Cyclic thrombocytopenia of acute type.


Problems of establishing the etiologic roles of hepatitis "candidate" viruses.


Tumours of salivary glands.

Tumours of salivary glands.


Craniosynostosis. Review of the literature and indications for surgery.


Experience with a human cytomegalovirus complement fixing antigen.


The isolation of vesiculay stomatitis virus (Indiana strain) and other viruses from mosquitoes in New Mexico, 1965.


Strategic viral surveillance of sewage during and following an oral poliovirus vaccine campaign.


The virus watch program: a continuing surveillance of viral infection in metropolitan New York families. V. Observations in employed adults on etiology of acute upper respiratory disease and heterologous antibody response to rhinovirus.


Respiratory viruses in Jamaica: a virologic and, serologic study. 1. Virus isolations and serologic studies on clinical specimens.


Reovirus antibody patterns in Alaska.

Reovirus antibody patterns in Alaska.


Vaccination of day-old chickens against infectious laryngotracheitis by conjunctival instillation.


Isolation of equine rhinopneumonitis virus from an epidemic of acute respiratory disease in horses.


Factors controlling the concentration of mitochondrial oxaloacetate in liver during spontaneous bovine ketosis.


Phosphatidylmyoinositol monomannoside in Propionibacterium shermanii.


Optical and magnetic properties of Pseudomonas azurins.


Ribonucleotide phosphorylating enzymes (kinases) in Escherichia coli, uninfected and infected with RNA-bacteriophage f2.


Reversible reaction of epsilon-amino groups of cytochrome c with salicylaldehyde to produce cytochrome c polymers.


Characterization of avian leucosis group-specific antigen from avian myeloblastosis virus.


Analysis of a thermal equilibrium phenomenon between high-spin and low-spin states of ferrimyoglobin azide.


Studies on acid hydrolases. V. Isolation and characterization of spleen nucleoside polyphosphatase.


Studies on acid hydrolases. IV. Isolation and characterization of spleen exonuclease.


The recognition of viral RNA by mammalian ribosomes. An effect of interferon.


Inhibition of the synthesis of 5-phosphoribosyl-1-pyrophosphate by 3'-deoxy-adenosine and structurally related nucleoside analogs.


Dihydro-5-azaorotic acid, the first specific inhibitor of dihydro-orotate dehydrogenase.


The effect of low concentrations of chlorampheicol on beating rat-heart cells in tissue culture.


Hepatic resection.

Hepatic resection.


Gas gangrene salpingo-oophoritis.

Gas gangrene salpingo-oophoritis.


Malignant melanoma of the penis: endolymphatic therapy with 131-I lipiodol.


Studies on the selectivity of antitumor agents.


Studies on coenzyme analogs. XV. A novel phosphorylating agent, P-diphenyl-, P'-Morpholino pyrophosphorochloridate.


Pattern of enzyme activity of nitrogen mustard-sensitive and resistant ascites tumors.


Relation of melanoma homogenate and ascorbate solution electron paramagnetic resonance doublets.


Evaluation of the complement fixation test for routine screening of amebiasis.


Herpes simplex virus hepatitis in mice: an electron-microscopic study.


Isolation of Herpesvirus hominis from naturally infected pet skunks.


Chemical mediators of adrenaline-induced pulmonary oedema.


Potential antiviral agents. Carbobenzoxy di- and tripeptides active against measles and herpes viruses.


Measurement of human luteinizing hormone in plasma by radioimmunoassay. The recent isolation of highly purified human pituitary luteinizing hormone (LH) has permitted the development of a sensitive and specific radioimmunoassay for this hormone in plasma. Results of this immunoassay system employing anti-LH serum agree closely with previous reports for the measurement of plasma LH in which immunoassays employing cross-reactive antisera to human chorionic gonadotropin were used. The immunoassay and bioassay of LH in several crude and partially purified pituitary and urinary extracts show acceptable agreement. The sensitivity of the LH immunoassay (0.2 mmug/ml) is adequate to measure LH levels in almost half of all prepuberal children and in all but a few normal adults. A small, but significant, rise in plasma LH level occurs at pubescence in both boys and girls. In women, plasma LH level varies with both age and the phase of the menstrual cycle. The mean LH concentration in nine normal women during the follicular phase (1.2 mmug/ml was found to be significantly higher than during the luteal phase (1.0 mmug/ml). At midcycle, the mean peak LH level was 10.2 mmug/ml. In a large group of normal women, the mean plasma LH concentration rose significantly at menopause to a level of 5.8 mmug/ml during the fifth decade and 10.5 mmug/ml during the seventh decade. A small, but significant, rise in plasma LH concentration also occurred in men from the third and fourth decades (0.7 mmug/ml to the seventh and eighth decades (1.7 mmug/ml). Both estrogen and testosterone suppress plasma LH levels, but marked variation in response exists. The immunoassay serves as a useful diagnostic tool in evaluating men with gonadal failure, amenorrheic women of reproductive age, and postmenopausal women suspected of hypopituitarism. From the half-time disappearance of LH-(131)I in plasma (mean 69 min) and the calculated volume of distribution (2.5-2.8 liters) it has been determined that approximately 30 mug of LH is secreted per day in men, and in women except at midcycle, at which time the release of LH is estimated to be 10-15 times this basal rate.


Characterization of the simian virus 40-specific RNA in virus-yielding and transformed cells.


Molecular basis of the action of interferon.


Products in the initial stages of digestion of polydeoxynucleotides by pancreatic deoxyribonuclease (DNase I).


Mucoepidermoid carcinoma of a minor salivary gland: report of case.


Destruction of rachitogenic activity of isolated soybean protein by autoclaving as demonstrated with turkey poults.


Experimental oral hepatography in rats.

Experimental oral hepatography in rats.


Cholestasis of pregnancy.

Cholestasis of pregnancy.


Significance of Herpesvirus hominis in respiratory secretions of man.


The effect of vagotomy and subsequent carotid arterial constriction on adrenocortical secretion.


Tyrosine transaminase induction in mammalian cells in tissue culture.


Oligonucleotide inhibition of amino acid attachment.


Translation of synthetic messenger RNA.

Translation of synthetic messenger RNA.


Rat intestinal microvillus membranes. Purification and biochemical characterization. 1. A technique is described for the removal of subcellular contaminants from intact rat intestinal brush borders, and for the subsequent separation of a microvillus membrane fraction from a fibrillar residue. 2. Increments in invertase activity, microscopic homogeneity and low nucleic acid content indicate that the microvillus plasma membrane has been extensively purified. Multiple membrane preparations have been shown to be highly reproducible with respect to their invertase specific activity, cholesterol content and phospholipid content. Alkaline phosphatase, leucine aminopeptidase, Mg(2+)- and Ca(2+)-dependent adenosine triphosphatase and seven separate disaccharidases were shown to be predominantly confined to the membrane fraction. 3. The fibrillar fraction has been shown to contain approximately 30% of the total protein of purified brush borders, plus most of the residual nucleic acid contaminant. No evidence was found for the localization of any specific enzyme in this fraction.


Investigations on vitamin D esters synthesized in rats. Turnover and sites of synthesis. 1. The tissue contents of vitamin D alcohol and ester were estimated in rats 5, 10, 24, 48 and 72hr. after peroral administration of [1-(3)H]cholecalciferol. 2. The total vitamin D in liver decreased in an exponential fashion from 19% of the dose at 5hr. to 0.6% at 72hr., but the ester content remained at a relatively constant low value from 5hr., so that by 72hr. it represented 67% of the total vitamin D. Vitamin D ester in kidney increased slowly to 48hr., but by 72hr. it was only 10% of the total vitamin D. 3. The small intestine, unlike liver and kidney, contained a higher content of vitamin D ester 10hr. after administration than at later times, and it is postulated that some vitamin D was esterified during absorption from the alimentary tract. 4. Plasma contained vitamin D ester at all time intervals, and it is suggested that ester found in liver and kidney could have been transported to these sites in the blood. 5. Thoracic-duct lymph was found to transport 43% of a peroral dose of vitamin D in 12hr., of which 1.4% was esterified. The fatty acid components of the lymph vitamin D ester, determined by two-dimensional thinlayer chromatography, were mainly palmitate (31%), stearate (25%), oleate (16%) and linoleate (16%). This pattern was similar to that previously found in liver.


Investigations on vitamin D esters synthesized rats. Detection and identification. 1. Vitamin D-deficient rachitic rats were given [1-(3)H]cholecalciferol by gastric intubation. After 24hr., diethyl ether extracts of liver and kidney contained 5-11% and 4.5-20% respectively of total vitamin D apparently esterified with long-chain fatty acids. 2. A two-dimensional thin-layer chromatographic technique was devised that completely separated seven synthetic vitamin D esters according to the chain length and number of double bonds in the fatty acid component. When the ;vitamin D ester' fraction from liver or kidney was co-chromatographed with the standard esters, radioactivity appeared mainly in vitamin D palmitate, stearate, oleate and linoleate regions. The proportion of radioactivity in the saturated fatty acid esters was higher in kidney than in liver. 3. The same percentage of tissue vitamin D in the esterified form was found at each of two dosages of vitamin D. 4. The possible specificity of a vitamin D esterification mechanism is discussed.


The chemical composition of the membranes of protoplasts and L-forms of Staphylococcus aureus. Membrane fractions were prepared from Staphylococcus aureus H and 100 after dissolution of the cell walls by a lytic enzyme from Streptomyces griseus. Membranes were also prepared from the L-forms derived from the same strains. The membranes were analysed for protein, lipid, carbohydrate and RNA contents, and the fatty acid composition of the lipids was determined. A branched-chain saturated C(15) acid was the major component in all samples, and the correspondence between L-forms and parent bacteria was fairly close. The lipids were separated into non-polar-lipid, glycolipid and phospholipid fractions; the L-forms contained a little more neutral lipid and much more glycolipid than the parent bacteria. In all membranes the glycolipid, which accounted for all the carbohydrate present, was a diglucosyl diglyceride. The major phospholipids of the protoplast membranes were phosphatidylglycerol and some lipoamino acids (lysine and a little alanine). On the other hand, diphosphatidylglycerol was the chief phospholipid found in L-form membranes.


The nature of copper in pig plasma benzylamine oxidase.


The diphosphoinositide kinase of rat brain. 1. The supernatant fraction of adult rat brain contains a diphosphoinositide kinase. 2. Formation of triphosphoinositide by the enzyme in the presence of ATP and Mg(2+) ions was shown with labelled ATP or labelled diphosphoinositide. 3. The kinase was also activated by Ca(2+), Mn(2+) and Co(2+) ions, but to a smaller extent than by Mg(2+) ions. 4. In the presence of optimum Mg(2+) ion concentration the enzyme was inhibited by Ca(2+) ions. 5. Activity did not depend on thiol groups and the pH optimum was 7.3. 6. The dialysed supernatant fraction had no diglyceride kinase activity and negligible phosphatidylinositol kinase activity. 7. Triphosphoinositide phosphomonoesterase was present but showed little activity under the conditions used to assay the kinase. 8. Diphosphoinositide kinase was purified by ammonium sulphate fractionation, ethanol treatment and chromatography on Sephadex G-200. 9. This purification removed much of the triphosphoinositide phosphomonoesterase.


Isopentenyl pyrophosphate isomerase from liver. Isopentenyl pyrophosphate isomerase (EC 5.3.3.2) was purified from extracts of pig liver by ammonium sulphate fractionation and by gel filtration. After about 20-fold purification the preparations were free of phosphatase and prenyltransferase (EC 2.5.1.1), the two enzymes that could have interfered with the assays. The isomerase has a distinct pH optimum at 6.0 and is activated by Mn(2+) in preference to Mg(2+). The K(m) value for isopentenyl pyrophosphate is 4x10(-6)m. The equilibrium of the reaction favours the formation of dimethylallyl pyrophosphate. The reversibility of the isomerase reaction was demonstrated directly by the formation of isopentenyl pyrophosphate from dimethylallyl pyrophosphate. It is suggested that two prenyl isomerases might exist, one involved in the synthesis of trans- and another in the synthesis of cis-polyprenyl substances.


The polymerization of adenosine 5'-diphosphate-ribose moiety of NAD by nuclear enzyme. II. Properties of the reaction product.


Association of neutral deoxyribonuclease with chromatin isolated from mammalian cells.


RNA with high template activity from leukaemic myeloblasts (BAI strain A virus-induced avian myeloblastosis). I. Optimal conditions for its isolation and activity in the subcellular protein-synthesizing system.


RNA with high template activity from leukaemic myeloblasts (BAI strain A virus-induced avian myeloblastosis). II. Sedimentation profiles of template activity and of rapid 32P labelling.


Cytomegalic inclusion disease. A case report.


Methods for the determination of adipose cell size in man and animals. Four methods for the sizing of adipose cells in small samples of human or animal adipose tissue are compared. These methods depend on the preparation of cell suspensions by incubation of the tissue with collagenase or by prolonged fixation with osmium tetroxide and separation of the fixed cells. A Coulter electronic counter was used to count and size the suspended cells and a Zeiss particle size analyzer for the sizing of cells in photomicrographs. The use of the Coulter counter to count cells in a suspension derived from a known amount of tissue and subjected to osmium tetroxide fixation is recommended for accuracy and general applicability to adipose cells of all sizes in man and animals.


Improved separation of sterols by column chromatography. Some closely related sterols have been separated on columns of neutral alumina-Super-Cel impregnated with silver nitrate. Most notable are the separations of some Delta(8)-sterols from their Delta(7)-isomers, separations which have been difficult, if not impossible, to achieve with other published column chromatographic methods.


Chromatographic separation of plasmalogenic, alkyl-acyl, and diacyl forms of ethanolamine glycerophosphatides. The plasmalogenic, alkyl-acyl, and diacyl forms of ethanolamine glycerophosphatides were completely separated from each other as methylated dinitrophenyl derivatives by thin-layer chromatography on Silica Gel G. The relatively high resolving power needed was obtained by multiple unidimensional development with solvents that give very low mobility to the lipids. Under these conditions the plasmalogens moved fastest, the alkyl-acyl lipids were intermediate, and the diacyl lipids were the slowest. The presence of all these forms of lipids in the ethanolamine phosphatides of hen's eggs, ox brain, and human blood plasma could be directly demonstrated with the new method.


6-acyl galactosyl ceramides of pig brain: structure and fatty acid composition. Two glycolipids were isolated from pig brain and were shown to be the fatty acid esters of kerasin and cerebron in which the second fatty acid moiety is attached to the 6-position of the galactose. The point of attachment was shown in two ways: by permethylation and by cleavage with periodate. Methanolysis of the permethylated cerebroside esters yielded O-methyl sphingosines, methyl esters of nonhydroxy or 2-methoxy acids, and methyl 2,3,4-trimethyl galactoside. Cleavage of the cerebron ester with periodate, followed by treatment with sodium borohydride and dilute HCl, yielded ceramide plus 1-monoglyceride. The ester-linked fatty acids were primarily 16:0, 18:0, and 18:1, while the amide-linked fatty acids showed the wide assortment of chain lengths typical of brain cerebrosides. The methylation step, with silver oxide and methyl iodide, yielded two derivatives with the cerebroside esters, but the structural explanation for the difference was not elucidated. The galactose in the cerebron ester was shown to exist in the beta-pyranoside form.


Hydrolysis of primary and secondary esters of glycerol by pancreatic juice. The relative rates of hydrolysis of the secondary ester in glycerol 1,3-benzylidene 2-oleate and in glycerol 1,3-dihexadecyl ether 2-oleate, and of the primary and secondary esters in triolein were determined. Both unaltered and selectively inactivated rat pancreatic juice were used as sources of enzyme. It was found that rat pancreatic juice contains an enzyme that can hydrolyze fatty acids esterified at the 2-position of a glyceride. This enzyme is not pancreatic lipase. It may be sterol ester hydrolase. Partial glycerides, as well as complete glycerides, can serve as substrates. Pancreatic lipase, if it can hydrolyze the 2-positioned fatty acids of a triglyceride, does so at a very slow rate.


Measurement of renin in human plasma.

Measurement of renin in human plasma.


Increase in transmitter content of adrenergic nerves in the reproductive tract of female rabbits after oestrogen treatment.


Vascular connective tissue under the influence of oestrogens. 3. Effect of oestrogens on experimental arteriosclerosis. Morphological and biochemical studies.


Some cooperative studies for the future.


Cultivation of "difficult" viruses from patients with common colds.


Necrotic cervicitis due to primary infection with the virus of herpes simplex.


2 NADH2 dehydrogenases in the basidiomycete Oudemansiella mucida.


Effect of silica dusts on macrophage permeability studied with 86RbCl.


Electron spin resonance studies of catalase and some of the catalase compounds.


Inhibition of oxidizing enzymes by metronidazole.


Prolongation of skin homograft survival by local intralymphatic radioisotope injections.


Adrenocorticotropic effect in humans of a synthetic beta-1-24 corticotrophine derivative.


Effects of a treatment with coenzyme A, alpha-lipoic acid, diphosphopyridine nucleotide and cocarboxylase on endogenous hepatic coma.


Stereospecificity in the enzymatic conversion of delta-7-cholesten-3-beta-ol to 7-dehydrocholesterol.


The conversion of L-glycerol-14-C 3-phosphate into phosphatidic acid by a solubilized preparation from rat brain.


The role of arginine in the replication of herpes simplex virus.


The ribonucleic acids of the infective and interfering components of vesicular stomatitis virus.


Isolation of polyoma virus and Semliki Forest virus from tissue culture fluid by continuous flow zonal ultracentrifugation.


Fractionation of polyoma virus DNA on hydroxyapatite: dependence on tertiary structure.


Experimental studies on therapeutic effects of various combinations of antituberculosis drugs. II. Comparison of various regimens in treatment of experimental mouse tuberculosis infected with SM- and INH-resistant Schacht strain.


On the possibility of lysophosphatide formation during Wallerian degeneration.


Specificity of assay of equine infectious anemia virus in horse leukocyte culture.


Studies on transmissible gastroenteritis in pigs. 3. Isolation of cytopathogenic viurs and its use for serological investigation.


Sialic acid of uterine muscle membrane: reappraisal of the serotonin receptor of smooth muscle.


Distribution of some dehydrogenase in the cerebellar cortex.


Isolation of a new toxin from cultures of Aspergillus flavus.


Glucocorticoid-induced pancreatitis in children.


Resection of metastases in Wilms' tumor: a report of three cases cured of pulmonary and hepatic metastases.


Treatment of malignant sympathetic tumors in children: clinicopathological correlation.


The de-segregation of the psychiatric patient.


Studies on periodic-acid-Schiff positive substance in the thymus of mice.


The relationship between the state of nerve-terminal polarization and liberation of acetylcholine.


Acetylcholine, bretylium and release of norepinephrine from sympathetic nerve endings.


Advances in connective tissue research and their relationship to the aetiology of periodontal disease.


The role of the hydrophobic bonding in P-450 and the effect of organic compounds on the conversion of P-450 to P-420.


Complement fixation reaction and agar gel double diffusion test in Tyzzer's disease of mice.


Relationship of orbital sialic to endocrine exophthalmos.


The heart in acute leukemia. A study of 420 autopsy cases.


Rhinovirus infections in an industrial population. 3. Number and prevalence of serotypes.


Live and inactivated adenovirus vaccines for the prevention of acute respiratory illness in naval recruits.


Characteristics of two enteroviruses isolated from swine with diarrhea.


Effects of chronic residence on size and size distribution of plaques of type A foot-and-mouth disease virus in primary calf kidney cell cultures.


Primary lesions in cardiac ganglia of the pig after herpesvirus suis infection.


Determnation of rat liver microsomal glucose-6-phosphatase activity: study of citrate and G-6-P inhibition.


Growth of seventy-eight recurrent mammary cancers. Quantitative study.


Thyroid lymphography of the dog.

Thyroid lymphography of the dog.


In vitro neoplastic transformation of hamster pineal cells by three oncogenic DNA viruses.


Post-hospital treatment of the psychotic patient.


Ascorbic acid depletion and corticosterone production in the avian adrenal gland.


Serodifferentiation of poliovirus strains. I. Modified Wecker test on types 1 and 2 poliovirus strains.


Oncogenicity of Schmidt-Ruppin strain of rous sarcoma virus in cynomolgus monkeys.


Oxidation of reduced nicotinamide adenine dinucleotide by melanin.


Studies of phosphorus metabolism by isolated nuclei. XI. Evidence for a multiplicity of nucleolar nucleoside diphosphatases.


Autoantigenic properties of proteins denatured by colloidal silicic acid.


Photochemistry of biological molecules. II. Photolysis of dipeptides in the solid state.


Kinetic behavior of EPR signal. I. Induction effects in intact algae.


The effect of chronic alcoholism on ketosteroid and hydroxycorticoid excretion.


Some effects of x-irraation on circulating eosinophils in rats: a short communication.


Werner's syndrome.

Werner's syndrome.


Studies in glycogen storage disease. II. Heterogeneity in the inheritance of glycogen storage diseases.


Aldosterone in clinical medicine.

Aldosterone in clinical medicine.


Microbial models of tumor metabolism.

Microbial models of tumor metabolism.


Enzyme histochemistry of ovarian tumors.


Relationship of the nicotinamide adenine dinucleotide glycohydrolase activity to nicotinamide adenine dinucleotide content and rate of proliferation of Ehrlich ascites tumor cells.


The use of nicotinamide to modify the toxicity of streptozotocin diabetes without loss of antitumor activity.


The prenatal exposure of mice to urethan and the consequent development of tumors in various tissues.


Location and characteristics of the phenylalanine transport mechanism in normal and polyoma-transformed hamster cells.


Dose-response characteristics of aflatoxin B1 carcinogenesis in the rat.


The epidemiology of Burkitt's tumor.

The epidemiology of Burkitt's tumor.


Immunofluorescence, interference, and complement fixation technics in the detection of the herpes-type virus in Burkitt tumor cell lines.


Formal discussion: immunologic relationships of the herpes-like EB virus of cultured Burkitt lymphoblasts.


Terrapene heart (TH-1), a continuous cell line from the heart of the box turtle Terrapene carolina.


Insulin inhibition of hormone and theophylline induced glycerol release in isolated adipocytes.


Fine structural cytochemical localizations of phosphatase activities of rat and guinea pig.


The distribution of constituents and enzyme activities in subfractions of liver microsomes from non-starved rats.


Chromosomal damage after infection with poliomyelitis virus.


A morphologic study on the chromatin areas associated with the nucleolus.


Purification of interferon from chick embryonic allantoic fluids and fibroblast tissue infected with influenza virus.


The inhibition of polyoma antigen synthesis and capsid formation by 5-fluorodoxyuridine.


Replication of PARA (defective SV40)-adenoviruses in simian cells.


Chromatographic separation of the various forms of polyoma virus DNA.


Replication of Riley's plasma enzymes elevating virus in tissue culture: the importance of the cellular composition.


Increase of lactic dehydrogenase and neutralizaing antibody in plasma of chickens infected with Rous sarcoma virus.


Thermo-efficient strains of variola major virus.


The supercoiling of papilloma virus DNA.


Enhanced severity of experimental Herpes simplex infection in mice fed a protein-free diet.


On the ganglionic effect of trypsin.

On the ganglionic effect of trypsin.


Surgical aspects of salivary gland disease.


Wilms' tumor and neuroblastoma. The child under treatment.


Malignant cystosarcoma phyllodes in a pre-pubertal female.


Capillary, mast cell and connective tissue response to myxoviruses in the rat.


Intratypic serodifferentiation of polioviruses by comparison of neutralization indices and correlation of the antigenic marker, temperature marker and monkey neurovirulence.


Free radicals of biological interest. II. Electron spin resonance (ESR) spectra of 2537 A irradiated amino acids.


Biosynthesis of chloramphenicol. II. p-Aminophenylalanine as a precursor of the p-nitrophenylserinol moiety.


Automated method for following the production of corticosteroids by adrenal tissue in vitro.


Procedure for determination of aldosterone in human peripheral plasma by double-isotope derivative assay, and its application for measurement of secretory rate and urinary excretion.


Role of intraneuronal vesicles in the synthesis, storage, and release of catecholamines.


Personality patterns in peripheral neuropathy.


Growth behavior of hepatomas in o-aminoazotoluene-treated mice in comparison with spontaneous hepatomas.


Enzymatic omega-oxidation. 3. Purification and properties of rubredoxin, a component of the omega-hydroxylation system of Pseudomonas oleovorans.


The antigenicity of native and tyrosylated neutral-salt-soluble rat collagen.


Kinetics of binding of cyanide to sperm whale metmyoglobin.


Probable murine origin of a virus (HHLV-2) recovered from leukemic mice after inoculation of human leukemic material.


Preventive rehabilitation in leprosy.

Preventive rehabilitation in leprosy.


Clinical experience in the neurologic substance of pain.


The transmission and central projection of pain.


Antimicrobial therapy in the newborn infant.


Polymyxins, colistin, bacitracin, ristocetin and vancomycin.


Improved dry-swab transportation for streptococcal specimens.


Antimicrobial agents: pediatric dosage, routes of administration and preparation procedures for parenteral therapy.


Dissociation constants of the binary complex of homogeneous horse liver alcohol dehydrogenase and nicotiniumamide adenine dinucleotide.


Inorganic pyrophosphate and the evolution of biological energy transformation.


Trace metal composition of photosynthetic bacteria.


Sulfhydryl content of rat ascites hepatomas with different sensitivity to x-rays and nitrogen mustard N-oxide.


Glucose-metabolizing enzymes in Yoshida sarcoma and its slow-growing variant subline.


Effects of angiotensin and catecholamines on the mesorchium microcirculation in the rat.


Effects of the administration of vitamin D2, D3, parathyroid hormone and calcium on hypocalcification of rabbit dentin and on changes in blood constituents caused by experimental rickets.


Programs for discharged patients. I. Community activities help readjustment.


Programs for discharged patients. II. Halfway house and day hospital complement each other.


Strange behavior of radiopaque substance.


Rescue of the defective murine sarcoma virus genome by radiation-induced leukemia virus from C57BL mice.


Sedimentation properties of mitochondrial and cytoplasmic ribosomal RNA's from Neurospora.


SV 40-specific thymidine kinase.

SV 40-specific thymidine kinase.


Temperature sensitivity of polyoma virus, induction of cellular DNA synthesis, and multiplication of transformed cells at high temperature.


Enzymatic joining of DNA strands, II. An enzyme-adenylate intermediate in the dpn-dependent DNA ligase reaction.


The enzymatic repair of DNA, II. Characterization of phage-induced sealase.


Charge transfer and electronic mobility.


Effect of bacteriophage infection on the sulfur-labeling of sRNA.


Inducers of interferon and host resistance, IV. Double-stranded replicative form RNA (MS2-Ff-RNA) from E. coli infected with MS2 coliphage.


Pox in wild birds, an annotated bibliography.


Mycoplasma pneumoniae and adenovirus respiratory illnesses in military and university personnel, 1959-1966.


Use of the pectoralis minor muscle in augmentation mammoplasty.


Cytologic diagnosis of pancreatic cancer by ductal aspiration.


Hospital differences in the release of discharge ready chronic schizophrenics.


On the use and abuse of LSD.

On the use and abuse of LSD.


Histochemical study on chromatolytic neurons.


Histamine formation in normal, regenerating and malignant liver tissue of the rat.


Pelvic arteriography in the management of chorioadenoma destruens.


Studies of the oxygenated compound of cytochrome oxidase.


Biosynthesis of the peptidoglycan of bacterial cell walls. IX. Purification and properties of glycyl transfer ribonucleic acid synthetase from Staphylococcus aureus.


A new pteridine from Tetrahymena.

A new pteridine from Tetrahymena.


Studies on the respiratory chain-linked reduced nicotinamide adenine dinucleotide dehydrogenase. 13. Binding sites of rotenone, piericidin A, and amytal in the respiratory chain.


Studies on the respiratory chain-linked reduced nicotinamide adenine dinucleotide dehydrogenase. XIV. Location of the sites of inhibition of rotenone, barbiturates, and piericidin by means of electron paramagnetic resonance spectroscopy.


Immunofluorescent studies of mouse and hamster cell surface antigens induced by polyoma virus.


Morphological investigation of human breast cancer.


Serologic evidence for human infection with adenovirus-associated viruses.


Susceptibility of cells transformed by polyoma virus and simian virus 40 to the cytotoxic effect of the carcinogenic hydrocarbon benzo[a]pyrene.


Virus-induced rhabdomyosarcoma transplantation studies in weanling BALB-c mice.


Immunogenetic aspects of polyoma virus susceptibility in mice.


Studies on the hamster-brain passage virus recovered from human lymphoma cultures.


Uridine nucleotides in brain.

Uridine nucleotides in brain.


Enzymes of phosphoinositide metabolism during rat brain development.


Effect of glucose and fructose administration on lipid metabolism in the rat.


Metabolism of diethylpropion-1-C14 hydrochloride by the human.


Familial XY gonadal dysgenesis.

Familial XY gonadal dysgenesis.


Acute hemorrhagic cystitis in children. Isolation of adenovirus type II.


Lymphography with chlorophyll: effects on pelvic lymphadenectomy and lymph nodes.


The possible etiologic relationship of virus to human leukemia.


Yields in the radiation degradation of solid carbohydrates.


Estimation of gestational age. Comparison between radiological assessment and maturity scoring.


The assay of milk-ejecting activity in the lactating rat.


The antihypertensive properties of 1-amino-4-phenyl pyridinium chloride.


The effect of lipolytic hormones and theophylline on heat production in brown adipose tissue in vivo.


Activity of ribonuclease, acid phosphatase, and phosphodiesterase in Tetrahymena pyriformis during growth.


Formation of membrane-glycogen arrays in rat hepatoma cells.


An electron microscopic study of the chorioallantoic membrane following infection with Rous sarcoma virus. Infection of the chick chorioallantoic membrane (CAM) with Rous sarcoma virus (RSV) has been thought by earlier workers (12, 20) to result in the transformation of the ectoderm and then the mesoderm of that organ. In the present study, CAM were infected with 10(4) PFU (pock-forming units) of RSV (Bryan high titre strain) and collected for electron microscopy at 2, 4, and 6 days postinfection. Observations of the fine structural changes in the CAM after RSV infection support a singular role of the mesenchyme in the initiation of the tumors. The ectodermal hyperplasia often associated with RSV tumors of the CAM appears to be a secondary response to the alteration of the underlying mesenchyme. These findings are discussed in detail, and an alternate course of RSV transformation of the CAM by way of the vascular bed is suggested.


Biochemical studies of transitions from rest to activity.


The influence of neural activity on neural metabolism of glucose and phospholipid.


Studies on the serological and interfering properties of avian leucosis virus isolates from field outbreaks of disease and from three vaccines.


Rare incidence of malignant renal tumours in infancy and childhood and long-time survival.


Malignant tumours of the kidney and ureter.


The analysis of individual molecular species of polar lipids.


Selective agents in cultivation of clostridia.


Comparison of iv and ia infusion of vasoactive agents on dog forelimb blood flow.


Interrelationship between angiotensin and vascular alpha adrenergic receptors.


The effect of photooxidation and histidine reagents on Murex trunculus haemocyanin.


Studies on the synthesis and intracellular accumulation of protocollagen in organ culture of embryonic cartilage.


Medical staff conference. Herpes simplex meningoencephalitis. These discussions are selected from the weekly staff conferences in the Department of Medicine, University of California Medical Center, San Francisco. Taken from transcriptions, they are prepared by Drs. Martin J. Cline and Hibbard E. Williams, Assistant Professors of Medicine, under the direction of Dr. Lloyd H. Smith, Jr., Professor of Medicine and Chairman of the Department of Medicine.


Influence of pH on the conformation of angiotensin II and analog peptides.


Abnormal response of the pulmonary artery of the rabbit after high frequency sympathetic nerve stimulation.


Dissociation of depolarization and ganglionic blockade induced by nicotine.


The actions of benzoquinonium in the isolated cat tenuissimus muscle.


Effects of 5-methylpyrazole-3-carboxylic acid (U-19425) and nicotinic acid on lipolysis in vitro and in vivo and on cyclic-3',5'-AMP phosphodiesterase.


Studies on the mechanism of the hypertensive effect of prostaglandin F2-alpha.


Analysis of antibodies against synthetic angiotensin II by the passive haemagglutination technique.


Differences in isozyme patterns of nucleic and cytoplasmic lactate dehydrogenase of rat liver cells.


Radioimmunoassay of angiotensin.

Radioimmunoassay of angiotensin.


Fine structure of nerve fibres in smooth muscle of the vas deferens in normal and reserpinized rats.


Effect of bromide ions on junctional transmission.


Adrenal-pituitary responsiveness during therapy with an oral contraceptive.


Familial aldosterone deficiency: enzyme defect, diagnosis, and clinical course.


Studies on the metabolism of angiotensin.


Pharmacologic assistance to the failing circulation.


An enzymic and fluorophotometric method for estimating urea concentrations in nanoliter specimens.


The enzymic determination of galacturonic acid.


The separation of nucleic acids on a modified methylated albumin-kieselguhr (MAK) column.


Penta-acid triglycerides of Chamaepeuce afra seed oil.


Structure of diene-phosphonous dihalide addition products and of derived phospholenes and phospholene oxides.


On the mechanism by which calcium and magnesium affect the spontaneous release of transmitter from mammalian motor nerve terminals. 1. The frequency of miniature end-plate potentials (m.e.p.p.s) was recorded from neuromuscular junctions in rat diaphragm phrenic nerve preparations in vitro after preparations had soaked in solutions containing Ca in concentrations between 10(-10) and 10(-2)M and a similar range of [Mg].2. Ethylenediamine tetra-acetate (EDTA) and ethyleneglycol bis (beta-aminoethyl ether) tetra-acetate (EGTA) buffers were added to prepare solutions with [Ca] and [Mg] below 10(-4)M. A computer program was used to estimate the free [Ca(2+)] in these solutions, and it was shown that the effects of Ca could be attributed to the free [Ca(2+)] in the bathing solution.3. M.e.p.p.s could still be detected without difficulty after soaking preparations for 6-8 hr in solutions containing EDTA or EGTA buffers and no added Ca. The basal frequency was unchanged upon exhibition of Ca in concentrations up to 10(-5)M and/or Mg in concentrations up to 10(-3)M.4. Ca in concentrations of and above 10(-4)M accelerated m.e.p.p. frequency from the basal level. This effect reached a maximum in [Ca] of 10 mM and raising the [Ca] above this level did not further change frequency. These effects were explained by the combination of Ca molecules with a nerve terminal receptor site. It was postulated that this combination allosterically activated the spontaneous release mechanism.5. Mg could accelerate m.e.p.p. frequency in the absence of added Ca. The interactions of Ca and Mg upon m.e.p.p. frequency indicated that Ca and Mg competed for the same sites.6. Raising the [H(+)] of the bathing medium accelerated m.e.p.p. frequency. This effect was thought to be exerted partly by combination with the same receptor sites as Ca and Mg and partly by variation of the ionization of the CaCl(2) of the bathing solution.


The effects of depolarization of motor nerve terminals upon the release of transmitter by nerve impulses. 1. Depolarizing currents were applied to motor nerve terminals in the rat phrenic nerve-diaphragm muscle preparation in vitro.2. During the passage of depolarizing currents the amplitude of the presynaptic nerve action potentials and of end-plate potentials (e.p.p.s) was reduced in proportion to the current strength.3. The reduction in e.p.p. amplitudes was shown to be due to a reduction in the number of quanta released.4. An excess of Mg ions or the previous application of a hyperpolarizing current could prevent the reduction of e.p.p. amplitudes and quantal contents by depolarizing current.5. Depolarizing current application prevented later hyperpolarizing currents affecting e.p.p. amplitudes.


The effect of adrenaline on the contraction of human muscle--one mechanism whereby adrenaline increases the amplitude of physiological tremor.


Disuse in the lateral geniculate nucleus of the cat. 1. An attempt has been made to produce disuse in the lateral geniculate nucleus (LGN) of the cat, in the synapses between the optic nerve fibres and the principal cells of the nucleus. Evidence is produced that destruction of the visual receptor cells by iodoacetate or 1,5-di(p-aminophenoxy) pentane dihydrochloride effectively silences the optic nerve discharge and so achieves this result.2. Disuse of the LGN synapses does not cause any decrease in synaptic efficiency. The LGN response to a single stimulus to the optic nerve was not appreciably altered, and the depression which normally follows single or repeated stimuli was much reduced or absent. This increased responsiveness was unaffected by prolonged tetanization of the optic nerve during the experiment.3. Two possible explanations of the increased responsiveness are suggested: a post-synaptic ;decentralization'-hypersensitivity and an increased output of transmitter per impulse. The relevance of these results to theories of learning is discussed.4. The LGN response of adult cats kept in complete darkness for periods of up to 966 days was not appreciably different from that in the normal cat and there was little or no increased responsiveness. This suggests that many retinal ganglion cells continue to discharge in total darkness for long periods. There is a possibility that disuse may develop after a long time in the dark.


The detection of renin in juxtaglomerular cells.


Permeation kinetics of glycerol and dimethyl sulfoxide in Novikoff hepatoma ascites cells.


Evidence for the vesicle hypothesis.

Evidence for the vesicle hypothesis. 1. The relationship of synaptic vesicles to the synaptic cleft was examined with the electron microscope at neuromuscular junctions in the rat diaphragm before and after bathing the preparation in a physiological salt solution for 2 hr.2. A population of vesicles was defined which appeared to ;touch' the axoplasmic membrane. These vesicles were found to be aggregated adjacent to axoplasmic densities which lay opposite the mouths of post-synaptic junctional folds.3. Soaking in the salt solution and modifications of this solution increased the proportion of folds opposed by presynaptic densities with associated vesicles.4. Soaking in solutions with 20 mM-KCl depleted both the specific vesicle population and the whole population of terminal vesicles. The effect was shown in paired experiments to be a specific effect of the 20 mM-KCl, and it was prevented by a concomitant increase of the bathing MgCl(2) concentration.5. Soaking in solutions with a raised osmotic pressure reduced the specific but not the general vesicle population.6. It is suggested that these observations support the vesicle hypothesis and that the specific vesicle population forms part of a feed-back mechanism adjusting transmitter synthesis and mobilization to the rate of release of transmitter.


Presynaptic depolarization of dorsal column fibres by adequate stimulation.


Evidence for structural changes during the action potential in nerves from the walking legs of Maia squinado.


The hepatic arteriography.

The hepatic arteriography.


Ultrastructural evaluation of the canine hypothalamic-neurohypophysial system in diabetes insipidus associated with pituitary neoplasms.


The lesions of porcine adenoviral infection in germfree and pathogen-free pigs.


An autoradiographic study of satellite cells in autonomic ganglia.


Serological responses of horses immunized with live attenuated African horse sickness vaccine.


Comparative study on neutralization tests in Japanese encephalitis.


"Pagetoid" change in the bronchial mucosa.


Primary hepatoma deoxyribonucleic acid from the Bantu.


Mixed tumors in ectopic sites.

Mixed tumors in ectopic sites.


Management of patients with Wilm's tumor.


Hernia repair: an out-patient procedure.


Comparison of clinical observations on patients infected with Entamoeba histolytica, with serological titres of their sera and virulence of their amoebae to rats.


Metastasis to the chamber angle from mucoid breast carcinoma.


Effect of postmortem interval on histochemical reactions of the myocardium.


Serum angiotensinase activity in cirrhosis.


Antihypertensive effect of an isolated phospholipid.


Sniffly schoolchildren.

Sniffly schoolchildren.


Prolonged peritoneal dialysis in patients awaiting renal transplantation.


Symposium on equine bone and joint diseases. Age and species differences in bone.


Studies with puromycin on the mechanism of negative feedback inhibition of ACTH secretion by glucocorticoids.


Development in hamsters of antibodies against surface antigens present in cells transformed by papovavirus SV40.


Auriculotemporal syndrome secondary to vertical sliding osteotomy of the mandibular rami: report of case.


Wilms' tumor in bone marrow aspirate.

Wilms' tumor in bone marrow aspirate.


The origin of acetylcholine released from guinea-pig intestine and longitudinal muscle strips. 1. Strips of longitudinal muscle can be obtained from guinea-pig ileum either retaining or free from Auerbach's plexus.2. The denervated strip is unresponsive to electrical stimulation by brief shocks, whether given singly or in trains; it also fails to respond to nicotine or dimethylphenylpiperazinium iodide (DMPP), and eserine causes no spasm.3. Denervated strips neither contain detectable acetylcholine (< 0.4 ng/mg), nor release it spontaneously (< 5 pg/mg/min) or in response to stimulation (< 31 pg/mg/min). The acetylcholine metabolism of the innervated strip is therefore that of the adherent Auerbach's plexus. Innervated strips had a mean acetylcholine content of 28 ng/mg, a mean resting output of 94 pg/mg/min and an output in response to stimulation at 10 c/s of 700-1200 pg/mg/min.4. By comparing the responses of innervated and denervated strips it was concluded that arecoline, methylfurmethide, alpha,beta-ethylal-gamma-tri-methylammonium propanediol iodide (2268 F), muscarine, histamine, tremorine, oxytocin, and substance P, like acetylcholine, act primarily on the smooth muscle directly; and that angiotensin, barium, potassium, m-bromophenyl choline ether and 5-hydroxytryptamine have a progressively increasing proportionate effect on the nerve plexus. Nicotine and DMPP were inactive in the absence of the plexus.5. The longitudinal muscle with its accompanying plexus contains about one quarter of the acetylcholine of the whole ileum, and is responsible for about one fifth of the output to electrical stimulation.6. The volley output of acetylcholine by the innervated strip declines sharply as rate of stimulation increases. Output of acetylcholine was reduced by morphine and by cocaine, particularly when resting or when stimulated at low rates.7. Acetylcholine output by whole ileum from guinea-pig declines in the absence of glucose, but is insulin-independent. Output by strips of ileum from rats made diabetic with alloxan was similar to that from normal rats.8. The similarity in properties of acetylcholine output from innervated strips, where it must come from nervous tissue, to that from whole ileum, and the insulin-independence of output from whole ileum suggest that the whole of the acetylcholine output of intestine is nervous in origin.9. Comparison of the acetylcholine metabolism of the innervated strip with that of the superior cervical ganglion suggests that the typical features of the former (high resting output, high volley output at low rates, low minute output at high rates of stimulation, and sensitivity to morphine) may be linked with the absence of specialized neuro-effector junctions and represent a relatively primitive transmission process.


Reticulospinal inhibition of transmission in reflex pathways. 1. The effect of electrical stimulation of the brain stem on reflex transmission has been investigated in decerebrate cats after partial transection of the spinal cord.2. Brain stem stimuli that do not evoke inhibitory post-synaptic potentials (IPSPs) in motoneurones or primary afferent depolarization may still effectively depress the excitatory and inhibitory synaptic actions evoked from the flexor reflex afferents (FRA) and from Ib afferents. There is no effect on post-synaptic potentials from Ia afferents or on Renshaw IPSPs. The depression is not associated with any measurable change in conductance over the motoneuronal membrane.3. There is also inhibition from the brain stem of transmission from the FRA (but not from Ia and Ib afferents) to primary afferent terminals and to ascending spinal pathways.4. It is concluded that this inhibition from the brain stem is exerted at an interneuronal level in spinal reflex paths.5. The inhibitory action is evoked from the region of Magoun's inhibitory centres in the brain stem and is mediated by axons with a conduction velocity of at least 20 m/sec. The axons are distributed in the dorsal part of the lateral funicle.6. The pathway mediating the inhibition from the brain stem is named the dorsal reticulospinal system. Its possible role in maintaining the decerebrate control of reflexes is discussed and related to the problem of a selective control of some paths from a primary afferent system.


Reticulospinal inhibition of interneurones. 1. The effect of electrical stimulation of the brain stem on interneurones in the dorsal horn and intermediary region has been investigated in decerebrate cats after partial transection of the spinal cord.2. Stimuli that effectively depress reflex transmission without giving a primary afferent depolarization inhibit the discharge evoked from the flexor reflex afferents in interneurones.3. Brain stem stimulation did not give post-synaptic potentials in the great majority of interneurones but effectively depressed the excitatory post-synaptic potentials (EPSPs) and inhibitory post-synaptic potentials (IPSPs) evoked from the flexor reflex afferents in these interneurones.4. IPSPs were, however, evoked in five of seventy-eight intracellularly recorded interneurones. These five interneurones were monosynaptically activated from primary afferents.5. It is tentatively postulated that a dorsal reticulospinal system inhibits reflex transmission by giving post-synaptic inhibition in first order interneurones. The results are also discussed in relation to effects on interneurones from other descending pathways.


The reflex regulation of arterial pressure during sleep in man.


Pituitary adrenocortical function in multiple sclerosis.


Control of ventricular fibrillation during induced hypothermia in cats after blocking the adrenergic neurons with bretylium.


Vasomotor responses in the hind limbs of foetal and new-born lambs to asphyxia and aortic chemoreceptor stimulation. 1. Hind limb blood flow was measured in lambs of from 91 days gestation (delivered by Caesarean section) to 1 month after birth (term is about 147 days), under chloralose anaesthesia. Vascular resistance/100 g wet wt. increased progressively with age. There was reflex femoral vascular tone from the earliest age studied, as shown by vasodilatation on cutting the sciatic nerve.2. On asphyxia by cord occlusion reflex femoral vasoconstriction began earlier and was somewhat greater in older foetal lambs. At all ages, and after denervation of the hind limb, there was vasodilatation after local ischaemia, and a vasoconstriction of delayed onset during asphyxia attributed to release of noradrenaline into the circulation. The vasoconstrictor effect of noradrenaline in immature lambs was at least as great as at term or in the new-born.3. Injections of minimal effective doses of cyanide were used to localize possible chemoreceptor sites in foetal lambs. Injection into the left atrium caused a rise of arterial pressure, femoral vasoconstriction and a complex change in heart rate (usually bradycardia) but rarely any respiratory movement. After atropine, cyanide caused a large tachycardia. All responses were much reduced or abolished by cervical vagotomy.4. Injection of the same doses of cyanide into a jugular vein, the right ventricle, pulmonary or common carotid arteries of foetal lambs caused negligible cardiovascular or respiratory effects, whereas injection into the carotids of new-born lambs caused a profound hyperpnoea.5. It is concluded that the aortic chemoreceptors are active in the foetus, are supplied from the left heart, and that they probably represent the primary defence in blood gas homeostasis by their effects on the circulation.


Angiotensin and tyramine tachyphlylaxis in isolated rat aorta.


Noradrenaline and sympathetic transmission.


Isolation of isopimpinellin from the fruits of Ammi majus L. natural coumarins. IV.


[Duodenal dyskinesia and digestive migraine].


The family of the surgical patient.

The family of the surgical patient.


Comparison of the traumatic deciduoma with the normal decidual reaction in mice.


The effect of reserpine implantation in subcortical structures on ACTH secretion and on locomotor activity.


Clearing-factor lipase in adipose tissue. Studies with puromycin and actinomycin. 1. When adipose tissue from starved rats is incubated in a medium containing glucose, insulin, heparin and actinomycin (5mug./ml.) the total clearing-factor lipase activity of the system increases at least tenfold over a period of 9hr. In the absence of actinomycin, enzyme activity also increases, but to a lesser extent and for only about 3hr. Some enzyme activity appears in the incubation medium in both the presence and the absence of actinomycin. 2. When the glucose and insulin of the incubation medium are replaced by pyruvate and heparin is omitted, an increase in the total clearing-factor lipase activity in the presence of actinomycin still occurs, but only after a lag of several hours. When only heparin is omitted from the medium, the rise in enzyme activity begins immediately, but there is a shoulder in the time-course curve after a few hours. In the absence of heparin, little enzyme activity appears in the incubation medium. 3. The increases in enzyme activity in the presence of actinomycin are prevented if puromycin (0.5mg./ml.) is present in the incubation medium. 4. Catecholamines and corticotrophin inhibit the increase in enzyme activity caused by actinomycin. 5. The clearing-factor lipase activity of adipose tissue from fed animals declines with a half-life of between 1 and 1.5hr. when the tissue is incubated in the presence of puromycin. The clearing-factor lipase activity of adipose tissue from starved animals is stable under similar circumstances, as is the raised activity found after such tissue has been incubated in the presence of actinomycin. 6. Clearing-factor lipase extracted from adipose tissue of fed animals is less stable in solution than that extracted from the tissue of starved animals after this has been incubated in the presence of actinomycin.


The kinetics of the reversible inhibition of heart lactate dehydrogenase through the formation of the enzyme-oxidized nicotinamide-adenine dinucleotide-pyruvate compounds. The inhibition of lactate dehydrogenase at high pyruvate concentration was studied in three ways. First, a rapid decrease in the rate of the enzyme reaction was observed; secondly, the rate of formation of a pyruvate-NAD(+) compound was followed by the change in E(325); thirdly, the rate of quenching of the protein fluorescence was measured. The data obtained at pH6.0 at different temperatures and ionic strengths as functions of pyruvate, NAD(+) and enzyme concentrations show that the extent of inhibition can be correlated with the reversible formation of a compound between pyruvate and enzyme-bound NAD(+). It is suggested that the detailed kinetic analysis of the formation of this abortive ternary compound will give pertinent information about properties of the enzyme-NAD(+) compound involved in the normal catalytic process.


Chloroplast and cytoplasmic low-molecular-weight ribonucleic acid components of the leaf of Vicia faba L. 1. A method for the extraction of plant nucleic acids and their separation on methylated-serum-albumin-kieselguhr columns is described. It is demonstrated that the characteristics of the elution profiles of material from the same source are consistently reproducible. 2. Major dissimilarities were found in the elution profiles of nucleic acids from root and from leaves of Vicia faba L. These dissimilarities were confirmed by polyacrylamide-gel electrophoresis. 3. Four distinct types of low-molecular-weight RNA were demonstrated to be present in leaves, clearly distinguished by their behaviour when chromatographed on methylated-serum-albumin-kieselguhr columns. (a) Both cytoplasmic and chloroplast ribosomes contained a low-molecular-weight RNA, and these components were distinct from each other. (b) The chloroplast possessed a unique ;soluble' RNA (i.e. RNA that is not precipitated by centrifugal forces that sediment ribosomes) which was not present in the rest of the cell. (c) A soluble component, probably transfer RNA, was found in both the chloroplasts and in the cytoplasm. 4. The components distinguishable by methylated-serum-albumin-kieselguhr column chromatography could not be distinguished by sucrose-density-gradient centrifugation.


Properties of a ribonucleate 2'-nucleotidohydrolase in a mouse lymphosarcoma.


Mitochondrial DNA. I. Preparation and properties of mitochondrial DNA from chick liver.


Adenyl cyclase of Brevibacterium liquefaciens.


A dual-label technique for comparing the rates of synthesis of nucleic acid fractions separated by methylated albumin-kieselguhr chromatography from cells in different states of activity.


Stability of radiation-induced organic free radicals. Decay by heat. The rate of free radical decay was measured at various temperatures using electron paramagnetic resonance spectroscopy. Rate constants determined from first-order decay kinetics were used to determine the activation energy for the process of free radical decay. The similarity between the temperature dependence of free radical decay by heat and that of electrical conductivity has led us to consider the possibility that the two processes may be related. Mechanisms by which a population of electron-hole conducting states may lead to free radical decay are outlined and experimental data relating to these mechanisms are discussed.


Studies on Brown FK. II. Degradative changes undergone in vitro and in vivo.


Enzymatic reactions at termini of DNA.

Enzymatic reactions at termini of DNA.


Presynaptic effect of oxygen on neuromuscular transmission in the frog.


An analogue device for computing variance of time intervals as a function of the mean.


Histochemical studies of the formazan reaction. I. A theoretical review of some of the factors that can affect the conversion of periodate-oxidized mucosubstances into formazans.


Histochemical studies of the formazan reaction. II. The conversion of periodate-reactive mucosubstances into diphenyl and phenyl-4'-diazo-3, 3'-dimethoxybiphenyl formazans and related derivatives.


Elements of neurography.

Elements of neurography.


Radiological notes. Bilateral Wilms' tumors.


A light and electron microscopy study of an unusual widespread nuclear inclusion body disease. A possible residuum of an old herpesvirus infection.


Peripheral nerve lesion in spongy degeneration of the central nervous system.


Simultaneous generalized cytomegalic inclusion disease and Pneumocystis carinii pneumonia. A case of a 2 year 5 month old girl.


Marine bacteria and their possible relation to the virus inactivation capacity of sea water.


The effect of angiotensin II infusion, renal hypertension and nephrectomy on salt appetite of sodium-deficient sheep.


The catecholamines in the central artery of the rabbit ear.


Studies on (Na+-K+)-activated ATPase. XIX. Occurrence and properties of a (Na+-K+)-activated ATPase in Escherichia coli.


Characterization of succinate semialdehyde dehydrogenase from rat brain.


Purification and properties of dichlorophenol-stimulated NADH oxidase from rat uterus.


The effect of some metabolites on glucose-6-phosphate dehydrogenase activity in loach embryos.


Inorganic pyrophosphatase activity of human placental alkaline phosphatase.


Activation and stability of lysosomal acid phosphohydrolase.


Desensitization of kidney fructose-1,6-diphosphatase by acetic anhydride.


Activation by electrolytes and polylysine of poly(A) synthesis by Clostridium perfringens polynucleotide phosphorylase.


The effects that patients have on their families in a community care and a control psychiatric service--a two year follow-up.


The effects of prostaglandins E1 and E2 on the smooth muscle of the dog spleen and on its responses to catecholamines, angiotensin and nerve stimulation.


Effect of corticotropin (ACTH) on tissue mast cell of rat.


Arrhenoblastoma of the ovary: a review of the literature and report of two cases.


Mouse cellular DNA enclosed in polyoma viral capsids (pseudovirions).


Production of SV40 virus in heterokaryons of transformed and susceptible cells.


Isolation of adenovirus-associated viruses from man.


An experimental model for internal denaturation of linear DNA molecules.


The keratosulfate-like mucopolysaccharide of chick allantoic fluid.


Flavoproteins of mitochondrial fatty acid oxidation.


Adenosine 3',5'-monophosphate in rat uterus: acute elevation by estrogen.


Inducers of interferon and host resistance. 3. Double-stranded RNA from reovirus type 3 virions (reo 3-RNA).


Tetanus.

Tetanus.


Bile acid chemistry. 3. Stepwise side-chain shortening by way of sodium per-iodate oxidation of alpha-hydroxy bile acids into corresponding aldehydes.


Congenital hypophosphatasia in a Nigerian neonate.


Irrigation of anterior chamber with neomycin-polymyxin solution in intraocular surgery.


Renal adenomas induced by dimethylnitrosamine. Enzyme histochemistry in the rat.


Further investigations by electron microscopy of herpes simplex virus in the corneal stroma of rabbits with experimental keratitis.


Lysosomal enzymes associated with vitreous hyalocyte granules. 1. Intracellular distribution patterns of enzymes.


Effects of electrocoagulation of the foetal lamb hypophysis on growth and development.


Antigenic relationship of the gram-negative organism causing canine abortion to smooth and rough brucellae. The use of whole-cell antigens in agglutination and agglutinin-absorption tests showed that the organism causing abortion in dogs is similar to rough Brucella abortus, B. melitensis, and B. ovis, but different from smooth Brucella cultures. Water-soluble antigens obtained by ultrasonic treatment and examined by immuno-electrophoresis and gel diffusion show extensive cross-reactions within the genus Brucella, but little or no cross-reaction with similar antigens from other gram-negative genera in the family Brucellaceae. The dog organism showed near identity with rough and smooth Brucella cultures on the basis of immuno-gel diffusion tests with water-soluble antigens, but it lacked the lipopolysaccharide-endotoxin associated with the agglutinogen of smooth brucellae. These findings support the proposal of Carmichael and Bruner for the designation of a new species, "Brucella canis."


Changes in plasma cortisol induced by dexamphetamine and chlordiazepoxide given alone and in combination in man.


Corticosteroids in the treatment of chronic asthma.


Restoration of the respiration inhibited by rotenone following the addition of glucose to Ehrlich ascites tumor cells. I. Effects of metabolic inhibitors.


On the role of ubiquinone in mitochondria. II. Redox reactions of ubiquinone under the control of oxidative phosphorylation.


A project to relieve overcrowding.

A project to relieve overcrowding.


The discharged chronic patient's utilization of health resources: a preliminary report.


The quantitative determination of some mitragyna oxindole alkaloids after separation by thin-layer chromatograppy. I. Ultraviolet spectrophotometry.


A chromatographic method for the quantitative determination of cholesterol 14-methylhexadecanoate (carcinolipin) in biological materials.