Effect of pituitary hormones on hepatic nicotinamide deamidase activity in hypophysectomized rats.


The cleavage of bile acid conjugates by cell-free extracts from Clostridium perfringens.


Clinico-pathologic correlation in early diagnosis of chorionic malignancy following hydatidiform mole.


Peripheral nerves of the forearm and hand--their repair and regeneration.


Clinicopathologic conference.

Clinicopathologic conference.


The intestinal flora in pigs with parakeratosis. V.


Functioning pancreatic islet-cell adenoma diagnosed preoperatively by means of splanchnic arteriography.


Phospholipids and active sodium transport in toad bladder.


Metabolism of phospholipids. IX. Phosphatidate phosphohydrolase in rat liver.


Incidence of intestinal amoebiasis.

Incidence of intestinal amoebiasis.


Drug-induced cytochemical changes in a glioblastoma.


Detection of viruslike particles in germ-free mice. Kajima, Masahiro (University of Notre Dame, Notre Dame, Ind.), and Morris Pollard. Detection of viruslike particles in germ-free mice. J. Bacteriol. 90:1448-1454. 1965.-Viruslike particles similar in structure to the mouse leukemia agent were detected by electron microscopy in thymus tissues of germ-free AK mice which had developed spontaneous leukemia. In addition, unique viruslike particles (type B) were detected in tissues from spontaneous mammary adenocarcinoma of germ-free C3H mice. Leukemia virus-like particles were also observed in the thymuses of the control AK mice and of the C3H control mice as well as of those with mammary tumors. Germ-free mice are not virus-free. The routes of transmission of leukemia as well as of mammary tumor viruses may be "vertical," through the embryo or placenta.


Quinone-independent reduction of cytochrome b-1 by reduced nicotinamide adenine dinucleotide in a purified soluble system from Escherichia coli.


Hepatoma metastasizing to the esophagus.


Renal pressor material in various hypertensive diseases.


The effects of varying doses of angiotensin on renal function and blood pressure in man and dogs.


Univalent fragments of antibody: a study by electron microscopy.


A pharmacoangiographic method for improving nephrophlebography.


Calcification in liver metastases from neuroblastoma.


Trachoma and follicular conjunctivitis in children.


Testicular androgen biosynthesis following corticotrophin and human chorionic gonadotrophin administration.


Interaction of macroions with the respiratory chain system of mitochondria and heart-muscle particles.


Glucose-6-phosphatase in human jejunal mucosa properties demonstrating the specific character of the enzyme activity.


Conversion of fructose to glucose by human jejunum absence of galactose-to-glucose conversion.


2-phosphoglyceric acid phosphatase: identification and properties of the beef-liver enzyme.


Effect of periodic dialysis on the peripheral neuropathy of end-stage renal failure.


Hormone structure and biological activity. Biochemical studies of three pituitary hormones.


Lack of correlation between body weight and cortisol secretion, and between adrenal weight and cortisol secretion in the dog.


Conservative surgical management of chorioadenoma destruens.


Pseudolipoma of the breast: a mask for cancer.


Manifestations of adrenal cortical carcinoma with high oestrogen excretion.


Dihydronicotinamide-adenine dinucleotide (NADH2) diaphorase, thiamine pyrophosphatase and acid phosphatase in the spinal cords of rabbits with chronic manganese poisoning.


Effects of infusion of catecholamines and angiotensin II on renin release in anesthetized dogs.


Recurrent peptic ulceration associated with pancreatic lesions (Zollinger-Ellison syndrome). Case report.


A direct photometric method for chloride in biological fluids, employing mercuric thiocyanate and perchloric acid.


Sex difference in rat liver uric acid metabolism.


ACTH secretion in midbrain-transected rats.


Genetic influence of the Rs locus on susceptibility to avian tumor viruses. I. Neoplasms induced by RPL-12 and three strains of Rous sarcoma virus.


Further studies on the fractionation of human pituitary glands.


Availability of niacin in wheat for swine.


Enzyme histochemistry of gastric carcinoma.


Pathogenesis of renal tubular necrosis induced by serotonin in the rat.


The development of the flora of the alimentary tract in young animals.


Arrhenoblastoma of the right ovary. Report of a case.


Hydatidiform mole in a 57-year old woman.


Induction of ovulation with clomiphene. Report of a study including comparison with intravenous estrogen and human chorionic gonadotrophin.


The effect of total-body and partial-body irradiation on granulocytic alkaline phosphatase in the rat.


Chemotherapy of advanced cancer of the cervix. Experience in 55 cases treated with cyclophosphamide.


Failure of FE2+-induced lipid peroxidation and swelling in the mitochondria isolated from ascites tumor cells.


The action of substituted guanidines on mitochondrial respiration and on the ADP-ATP exchange reaction.


Mannitol-1-phosphate dehydrogenase from the extracts of Lactobacillus plantarum.


Disruption of mitochondria and solubilization of cytochrome oxidase by NaOH and KOH.


Malignant non-chromaffin paraganglioma (report of two cases).


The control of inhibitory impurities in reduced nicotinamide adenine dinucleotide in lactate dehydrogenase assays.


Encephalitis associated with herpes simplex virus.


Herpes simplex encephalitis. Report of 20 cases.


Immunochemical study on antigenic components of Borketella pertussis.


Studies on protective antigen of Bordetella pertussis: isolation of protective antigen by sucrose density gradient centrifugation and some properties of the antigen.


Typing of the foot-and-mouth disease virus.


Persistence as a motivator.

Persistence as a motivator.


On the relationship between histamine sensitizing factor and mouse protective antigen.


The effect of sex differences and gonadectomy on some aortic enzymes of the rat.


Cardiovascular reactivity in rats with spontaneous inherited hypertension and constricted renal artery hypertension.


An evaluation of the radiotherapeutic management of intracranial glial tumors without prior surgery.


Cylindroma.

Cylindroma.


Radio immunoassay employing gel filtration.


Determination of heme a concentration in cytochrome preparations by hemochromogen method.


Ion-exchange thin-layer chromatography. XII. Quantitative elution and microdetermination of nucleoside monophosphates, ATP, and other nucleotide coenzymes.


In vitro polio virus inhibition by two guanidino-pyrimidines.


The effect of ACTH on cholinesterase activity in plasma, whole blood, and blood cells of rats.


Gangrene of the eyelids.

Gangrene of the eyelids.


Comparison of the inactivation and antigenicity of foot-and-mouth-disease virus by acetylethyleneimine and by combined effect of ultraviolet light and beta-propiolactone.


The relation between ionic fluxes and the action of drugs on smooth muscle.


Enterovirus hemagglutination: inhibition by several enzymes and sugars.


The blocking effect of West Nile virus on production of sarcoma by Rous virus in chickens.


The involvement of a tryptophan residue of glutamate dehydrogenase in the binding of L-glutamate.


The role of viruses in the etiology of chromosomal abnormalities.


Synthesis of microsomal membranes and their enzymic constituents in developing rat liver.


On the formation of thymidine-3'-triphosphate by a mammalian cell-free system.


Variable drug sensitivity and chromosomal features of a cancer clone.


Parathyroid hormone and metastasis of experimental tumor. I. Effect of parathyroid hormone on the metastasis formation of experimental tumor.


Labeling of mitochondrial phosphatidyl inositol phosphate by Pi32 and gamma P32ATP.


SHistochemical studies on beta-glucuronidase in normal and abnormal tissues employing naphthol AS-OL glucuronide as a substrate.


Infection of tumor cells by extraneous viruses. 3. Viral infection of tumors in homologous and heterologous hosts.


Base composition of RNA in hepatic subcellular fraction from rats fed carcinogenic aminoazo dye.


Handling of perchloric acid in laboratories and fume cupboards.


Activated silica gel as an adsorbent for atmospheric contaminants.


Induction of cellular DNA synthesis by polyoma virus. II. Increase in the rate of enzyme synthesis after infection with polyoma virus in mouse kidney cells.


Purification and chemical analysis of Shope papilloma virus.


A heat-sensitive cellular function required for the replication of DNA viruses but not RNA viruses.


Virus particles associated with "nonproducer" Rous sarcoma cells.


Cytological observations of "nonproducer" Rous sarcoma cells.


A cause of variation in clonal morphology of polyoma transformed hamster cells.


Partial nucleotide sequences in turnip yellow mosaic virus RNA.


Infectious poliovirus RNA: a sensitive method of assay.


Induction of SV40 T antigen with SV40 DNA.


Enhanced thymidine kinase activity following infection of green monkey kidney cells by simian adenoviruses, simian papovavirus SV40, and an adenovirus-SV40 "hybrid".


Infections acquired in medical wards. A report from the Public Health Laboratory Service.


A survey of enteroviruses and adenoviruses in the faeces of normal children aged 0-4 years. A report of the Public Health Laboratory Service and the Society of Medical Officers of Health.


The effect of intra-arterial hydrogen peroxide in rabbits infected with Clostridum perfringens.


Virilizing adrenal tumors in childhood. Report of a case.


Curriculum, thy name is change.

Curriculum, thy name is change.


There is a difference.

There is a difference.


Emergency à la carte to table d'hôte.

Emergency à la carte to table d'hôte.


Study to seek ways to meet rural nurse shortage.


The bridge between protected student and productive graduate.


SJH family centered maternity care based on naturalness, togetherness.


Cystosarcoma phyllodes: a review of three cases.


Surgical treatment of hyperinsulinism due to B-cell adenoma.


Metabolism of isolated fat cells. 3. The similar inhibitory action of phospholipase C (Clostridium perfringens alpha toxin) and of insulin on lipolysis stimulated by lipolytic hormones and theophylline.


Nicotinamide mononucleotide pyrophosphorylase activity in animal tissues.


On the magnetic resonance of spinach ferredoxin.


Structure of the chondroitin 4-sulfate-protein linkage region. Isolation and characterization of the disaccharide 3-O-beta-D-glucuronosyl-D-galactose.


Enzymatic studies on isolated nucleoli of rat liver.


Tracheal and tracheobronchial resections; technique and results in 20 cases.


Unexpected deaths from small bowel obstruction.


Recovery and identification of adenovirus in infections of infants and children.


The effect of stimulus intensity on latency of nerve action potential in healthy subjects and in patients with peripheral nerve lesions.


Hereditary compression syndrome of peripheral nerves.


Recovery and identification of human myxoviruses.


The clinical management of peripheral pain.


Multiple inhibition of yeast alcohol dehydrogenase.


Hydrophobic interactions in the binding of nicotinamide adenine dinucleotide to yeast alcohol dehydrogenase.


A "three gases technique" as a method for the determination of volatile hydrides and related compounds in a modified Warburg assay.


Vitamin D-3-sensitive Ca-45 binding by microsomal preparations from chick intestinal mucosa.


Myeloperoxidase of the leucocyte of normal human blood. 3. Isolation of the peroxidase granule.


Effect of corticotropin on the absorption of vitamin B12 in the subacute combined degeneration of the spinal cord.


The biosynthesis of adrenal steroids: effects of angiotensin II, adrenocorticotropin, and potassium.


Age changes in certain properties of the DNA of the small intestinal mucosa of white rats.


Structural and cytochemical organization of receptor cells of the sense organs in the light of their functional evolution.


Effect of repeated ACTH administration on 3-beta-ol-dehydrogenase activity and synthesis of steroids from progesterone in adrenal glands of rabbits.


Magnetic and optical properties of some bacterial haem proteins.


Oxygen evolution coupled to reduction of manganipyrophosphate ion by illuminated spinach chloroplasts.


Magnetic anisotropy of the myoglobin single crystal.


Electron spin resonance of haemoglobin MIwate and MOsaka.


The interaction of free radicals in protein and melanin.


Phosphatase activity during the growth and metamorphosis of Tribolium confusum Duval.


Studies on human platelet granules and membranes.


Absorption of vitamin D3-3H in control subjects and patients with intestinal malabsorption.


Immunological studies of human gliomas.

Immunological studies of human gliomas.


Perchlorate test in hyperthyroid patients treated with radioactive iodine.


Studies on the mechanism of antiviral activity of ketoaldehyde derivatives of biphenyl.


Encephalitis associated with adenovirus type 7 occurring in a family outbreak.


Bilateral mixed tumors of the palate.

Bilateral mixed tumors of the palate.


Hamster ascitic fluids containing complement-fixing antibody against virus-induced tumor antigens.


Retinal disease in neurology.

Retinal disease in neurology.


Inhibition of Sendai virus by N-1-isobutylbiguanide hydrochloride as studied by immunofluorescent staining.


Simian enterovirus SV2 hemagglutination studies. II. Effect of various treatments on the hemagglutinin and its cell receptors.


Responses of cell cultures to insecticides. 3. Altered susceptibility to poliovirus and diphtheria toxin.


Effects of oophorectomy and various doses of estradiol-17beta on corticosterone production by rat adrenal slices.


Thymidine kinase in IDU resistance.

Thymidine kinase in IDU resistance.


Succinoxidase activity of the liver of rats bearing Walker carcinoma 256.


Sodium and potassium intake, blood pressure, and pressor response to angiotensin.


Pathogenicity of murine hepatitis virus recovered from infant Swiss mice.


Distribution of vitamin D3-4c14 in rachitic and vitamin D3 treated chicks.


Zoster-like lesions from herpes simplex virus in newborn rats.


Spontaneous leukemia in germfree AK mice.


Generalized infantile herpes simplex. Report of an autopsy case with special reference to its relationship to Kaposi's varicelliform eruption.


Skeletal development of cleft palate children as determined by hand-wrist roentgenographs: a preliminary study.


Effects of norepinephrine and angiotensin II-amide on coronary flow and myocardial oxygen consumption in the cat.


Glucocorticoids for parenteral administration.


Physiological and patho-physiological variations, relation to pituitary-adrenocortical hormones, and to serum trypsin inhibitor.


Effect of phosphate on the activity of NADH-diaphorase from erythrocytes of hereditary methemoglobinemia.


Studies of adenovirus SV40 hybrid viruses. I. Assay system and further evidence for hybridization. The titers of adenovirus and SV40 genetic carriers in hybrid preparations (E46(+)) can be quantitated by determining the percentage of cells showing neoantigens stainable with fluorescent antibody (FA) at 21 to 24 hours; both titers can be obtained with a single coverslip. The adenovirus and SV40 antigen-inducing titers so obtained are of the same order of magnitude in stock preparations of E46(+) grown in either African green monkey kidney (AGMK) or human embryonic kidney tissue culture (HEK), and are generally within one log of the infectivity titer. Quantitative studies of 50 degrees C heat inactivation, ultracentrifugation, and equilibrium density gradient centrifugation of E46(+) gave no indication that SV40 neoantigen induction could be dissociated from adenovirus, whereas adenovirus and SV40 virus grown as a mixed infection were readily dissociated by these procedures. Pretreatment of HEK cells with a medium containing 5-fluorouracil desoxyriboside (FUDR) did not affect induction of either adenovirus or SV40 neoantigen or development of cytopathic effects after infection with E46(+), but did prevent formation of Ad. 7 viral antigen.


Studies of adenovirus SV40 hybrid viruses. II. Defectiveness of the hybrid particles. E46(+) virus purified by limiting dilution and plaque isolation in AGMK cells consists of a mixture of hybrid and non-hybrid virus particles. Of 102 plaque isolates obtained in HEK cells, 98 contained no detectable hybrid virus, while 4 contained a small proportion of hybrid virus. Plaque isolates from AGMK cells uniformly induced SV40 neoantigen, but when these plaque isolates were plaqued in HEK cells the progeny were again non-hybrid adenovirus. It thus appears impossible to obtain a pure line of hybrid virus. The proportion of hybrid virus produced in HEK cells fell progressively with decreasing multiplicity of infection, and plaque induction by E46(+) virus in AGMK followed 2-hit kinetics, indicating that the hybrid virus particles are defective and require dual infection with non-hybrid virus for their propagation.


Studies of a myxovirus recovered from patients with infectious hepatitis. A virus has been isolated from the serum and urine of patients with infectious hepatitis. This virus is a member of the parinfluenza group. It is serologically distinct from the known members of the group, but apparently is antigenically related to the simian type-2 parainfluenza viruses. A causal relationship between WB virus and infectious hepatitis has not been established.


Primary hepatoma associated with polycythaemia: report of a case with a study of tumour and urine erythropoietin levels.


Pleomorphic adenoma of the upper lip.

Pleomorphic adenoma of the upper lip.


The effect of certain hemostatic agents and the local use of diluted epinephrine on bleeding during oral surgical procedures.


Bronchiolar carcinoma: a report of six cases. Bronchiolar carcinoma is a malignant tumour which apparently arises in a terminal bronchiole from which it spreads either by bronchial embolization or by lymphogenous and/or hematogenous dissemination. It is not a common neoplasm.Histologically, the tumour bears a striking resemblance to the disease of sheep, jagziekte, which is of virus etiology. A very common finding in reported cases is preexisting pulmonary fibrosis. At the Nova Scotia Sanatorium, Kentville, 80 cases of primary lung cancer have been encountered within the past 25 years. Six of these were bronchiolar carcinomas. Five patients had co-existing chronic pulmonary disease, bronchiectasis in one and tuberculosis in four. One patient died of a rapidly progressive bilateral lesion and five were explored. Lobectomy was done in all five, but in one for palliation only. Three patients are alive and well three, six and 14 years, respectively, after their operations.


Retroperitoneal nonchromaffin paraganglioma with hormonal activity.


Studies on the development, biochemistry, and biology of experimental hepatomas.


Avian tumor viruses.

Avian tumor viruses.


Angiotensin and the liver.

Angiotensin and the liver.


Cardiovascular responses of the primate in endotoxin shock.


Cardiovascular drugs in muscular subaortic stenosis.


Factors affecting antibody transfer in the newborn puppy.


Effects of prolonged heat exposure on the cellular metabolism of the hamster.


Angiotensin.

Angiotensin.


Pulmonary cancer and adenomatosis in captive wild mammals and birds from the Philadelphia zoo.


Elevation of erythropoietin levels in association with Wilms' tumor.


Thrombotic thrombocytopenic purpura. Recovery after splenectomy.


Formation of oligonucleotides with 5'-phosphomonoester end group by rat-liver microsomal fraction.


The periodate-Schiff reaction: specificity, kinetics, and reaction products with pure substrates.


Ryan virus: a possible new human pathogen.


Non-specific effects of synthetic corticotrophin polypeptides.


Leukaemia and neoplastic processes treated with Langat and Kyasanur Forest disease viruses: a clinical and laboratory study of 28 patients.


Parotid fluid steroid responses to ACTH in surgically confirmed cases of Cushing's syndrome.


Nasopharyngeal angiofibroma: (a report of 21 cases treated surgically).


ACTH-like activity in a thymoma associated with gonadal dysgenesis.


Glucocorticoid suppression of urinary testosterone excretion in patients with idiopathic hirsutism.


Deoxycorticosterone acetate hypertension in dogs.


Tumors in newborn animals.

Tumors in newborn animals.


Results in the treatment of Wilms' tumour.


Response of tumorigenic viruses and of cells to biologically active compounds. I. Methods for determining response and application of methods.


The effect of age on the variations in the activities of glucose-6-phosphatase and fructose-1,6-diphosphatase in the liver of chicks.


Effect of cocaine on cardiovascular responses in intact dogs.


Spontaneous herpes-T infection in the owl monkey (Aotus trivirgatus).


Adrenaline synthesis: control by the pituitary gland and adrenal glucocorticoids. The activity of phenylethanolamine-N-methyl transferase, an enzyme that synthesizes adrenaline from noradrenaline in the adrenal medulla, is markedly depressed following hypophysectomy. Enzyme activity is restored to normal after administration of ACTH or the potent glucocorticoid, dexamethasone. Thus the biosynthesis of adrenaline in the adrenal medulla appears to be regulated by the pituitary-adrenocortical system.


Biosynthess of histones and acidic nuclear proteins under different conditions of growth. The incorporation of uniformly labeled L-lysine-C(14) into the normal and regenerating rat liver, into Novikoff hepatoma histones, and into acidic nuclear proteins was studied. In rat liver, different histone fractions incorporate labeled lysine to a different extent. Such differences become less obvious in regenerating liver, and they are even less so in Novikoff hepatoma. In the hepatoma cells the ratio of the biosynthesized acidic nuclear proteins to histones was altered.


Effect of sympathomimetic amines and angiotensin on active ion transport in smooth muscles.


A study by interference microscopy of the qualitative and quantitative changes in Entamoeba histolytica under different experimental conditions.


Further studies on the deletion of the cholesterol feedback system in hepatomas.


On protein binding of fluorenyl carcinogens by minimal deviation hepatomas.


A calcifying topical reaction to mast cell depletors in the rat.


Experimental bacterial peritonitis in mice.


The fine structure of the normal, neonatal mouse liver.


On the etiology of recurring oral ulcers.


The induction of mammary growth in the hypophysectomized goat.


Neoplastic transformation of rat embryo fibroblasts by fowl sarcoma virus B77.


Drug combinations in antibacterial chemotherapy. DOUBLE CHEMOTHERAPY FOR THE TREATMENT OF BACTERIAL INFECTIONS HAS BEEN RECOMMENDED FOR MANY REASONS, OF WHICH THE MOST IMPORTANT ARE THE FOLLOWING: (1) To achieve a synergic effect. (2) To delay the emergence of resistant strains. (3) To prevent superinfection. (4) To treat relatively inaccessible bacteria. (5) To treat mixed infections. (6) To treat undiagnosed infections.Combinations which have a truly synergic effect in vivo are those which show bactericidal synergy in vitro. Bactericidal therapy is of great practical importance in conditions which are inaccessible to the natural defences of the body, or where they are deficient. If in such cases the infecting bacterium is not readily killed by a single drug, then combinations should be tried. There are no absolute rules and double bactericidal sensitivity tests should always be carried out on the infecting strain, but the most likely combination to show this type of synergy is a penicillin and streptomycin.If a bactericidal drug is combined with an agent which is only bacteristatic, the killing effect may be antagonized, since many bactericidal drugs only kill rapidly multiplying cells. Again there are no absolute rules, but antagonism is particularly liable to occur when the bactericidal agent is one of the penicillins and is very unlikely to occur when it is a polymyxin, since this kills resting bacteria.Drug combinations to delay the emergence of drug-resistant strains should be considered for infections due to staphylococci and coliform bacilli, particularly in hospitals, and when it is desired to use drugs to which these bacteria readily develop resistance.Drug combinations may also be the most efficient treatment for mixed infections and may be necessary for the treatment of fulminating infections pending bacteriological diagnosis. The combination of nystatin with a tetracycline may be necessary to prevent candidiasis if tetracycline therapy has to be prolonged.


A histochemical study of hydrolytic and oxidative enzymes in an eosinophilic granuloma of parotid gland region.


Use of tetrazolium salts for electron transport studies in meningopneumonitis. I. Reduced nicotinamide adenine dinucleotide system. Allen, Emma G. (Downstate Medical Center, Brooklyn, N.Y.). Use of tetrazolium salts for electron transport studies in meningopneumonitis. I. Reduced nicotinamide adenine dinucleotide system. J. Bacteriol. 90:1505-1512. 1965.-Purified preparations of meningopneumonitis virus (MP) prepared from allantoic fluids of infected chick embryo reduce several tetrazolium salts in the presence of reduced nicotinamide adenine dinucleotide under both aerobic and anaerobic conditions. The pattern of reduction by MP differs from that seen in normal allantoic membrane homogenates, and is inhibited by several cations but not by KCN, atabrine, amytal, antimycin A, or 2,3-dimercaptopropanol (BAL). The reduction of cytochrome c by purified preparations of MP differs from its reduction of tetrazolium salts in that the cytochrome reaction is completely inhibited by BAL and partially inhibited by amytal, atabrine, and antimycin A. The cytochrome reductase of normal allantoic membrane preparations is completely inhibited by each of these compounds.


Nucleic acid-induced resistance to viral infection. Takano, Kouichi (Chas. Pfizer & Co., Inc., Terre Haute, Ind.), Joel Warren, Keith E. Jensen, and Alan L. Neal. Nucleic acid resistance to viral infection. J. Bacteriol. 90:1542-1547. 1965.-Administration of nonviral nucleic acids to mice increased their resistance to a subsequent infection with influenza or encephalomyocarditis viruses. Injection of ribonucleic acid or deoxyribonucleic acid by peripheral routes did not modify susceptibility to intranasal infection. Lung tissue extracts from animals previously treated with yeast nucleic acid inhibited the growth of vaccinia and influenza viruses. The protective effect of exogenous nucleic acids persisted in mice for several days, but gradually diminished to undetectable levels.


Incorporation of C14 from amino acids and peptides into protein by Clostridium perfringens type D. Hauschild, Andreas H. W. (University of Toronto, Toronto, Ontario, Canada). Incorporation of C(14) from amino acids and peptides into protein by Clostridium perfringens type D. J. Bacteriol. 90:1569-1574. 1965.-Uptake of C(14) from C(14)-labeled amino acids and peptides by Clostridium perfringens was measured in culture media containing acid or papain hydrolysates of C(14)-labeled Chlorella protein. Between 2 and 4 hr of growth, the rate of C(14) uptake from peptides was higher than from free amino acids. Peptides extracted from cells with hot ethyl alcohol contained six to nine times more C(14) after 4 hr of growth with C(14)-labeled peptides than with C(14)-labeled amino acids. Incorporation of C(14)-labeled glycine, serine, threonine, alanine, and proline into both cellular and exocellular protein was two to five times higher when these were supplied as components of dialyzable peptides rather than as free amino acids.


Previously unrecognized virus from submaxillary glands of gnotobiotic and conventional rats. Ashe, Warren K. (National Institute of Dental Research, Bethesda, Md.), Henry W. Scherp, and Robert J. Fitzgerald. Previously unrecognized virus from submaxillary glands of gnotobiotic and conventional rats. J. Bacteriol. 90:1719-1729. 1965.-A serially transmissible cytopathic agent was isolated from histologically normal submaxillary glands, but not from various other tissues or specimens, from 74 of 97 gnotobiotic and conventional rats. Triturates of the glands or subsequent culture supernatant fluids induced specific cytopathic effects (CPE) in monolayer cultures of primary rabbit kidney cells (14 passages), a line of human skin cells (8 passages), and HeLa cells (17 passages). Transfer of supernatant fluids containing infected cells enhanced transmissibility. Neutralization of the CPE was demonstrated with sera of gnotobiotic and conventional rats and with homologous rabbit antiserum. A cold hemagglutinin specific for rabbit erythrocytes is associated with, but separable from, the infectious particle. Cultures of the agent induced no discernible effect on inoculation by various routes into suckling, weanling, or adult conventional mice, rats, and hamsters. Weanling and adult rabbits were also unaffected. Cultures for bacteria in gland extracts and infected cell supernatant fluids were uniformly negative. Negative cultures on PPLO media and negative arginine deiminase tests indicated that this agent is not a Mycoplasma. The data indicate that it is a virus whose biological and physical properties distinguish it from the known cytomegaloviruses. Because it has been found so far only in rat submaxillary glands, this agent is designated provisionally as RSMG virus.


A sample holder for electron spin resonance studies on x-irradiated fibres.


Peptides for toxinogenesis of Clostridium perfringens type D.


The role of radiation therapy in the treatment of chemodectomas of the glomus jugulare.


Nasopharyngeal angiofibroma, an angiographic evaluation.


Multiple respiratory virus infections in hospitalized children.


Poliomyelitis in an isolated population: report of a type 1 epidemic in the Marshall Islands, 1963.


Some bactericidal and virucidal properties of iodine not affecting infectious RNA and DNA.


Histological studies of the monkey neurovirulence of group B arboviruses. I. A semiquantitative grading scale.


Clinical reactions following bronchography.


Mixed tumors of major salivary glands: prognostic role of capsular structure.


Skeletal and denture patterns in children from Yuendumu, Central Australia, and Melbourne.


The hypoglycaemic effect of mebanazine (Actomol) and its mechanism.


The estimation of the kallidins in blood and urine.


Actinomycetes activity against foot and mouth disease virus determined by a new method.


Contributions of electron microscopy to occupational health.


Biochemical changes in denervated skeletal muscle. 3. The effect of denervation atrophy on the concentration and 32P labelling of the individual phospholipids of the rat gastrocnemius.


The stimulation of free radical formation in Arthrobacter sp. by manganous ions.


"Fucidin" therapy in carcinoma of the breast. (Preliminary report).


Quinhydrone as quantitative standard for electron spin resonance spectrometry of biological systems.


Preliminary clinical study of mithramycin (nsc-24559) in primary tumors of the central nervous system.


The use of collagenase in investigating the synthetic balance of the intercellular materials.


Quantitative assay of granaticin by the microbiological diffusion method.


Bacteriuria during indwelling catheter drainage. Effect of constant bladder rinse.


Cytopathic effects and immunofluorescence produced by the J 121 strain of bovine Parainfluenza 3 virus in tissue culture.


A new adenovirus serotype of bovine origin.


Histochemical studies of some oxidative enzymes in parenchymal cell damage of the liver of rats fed on 3'-methyl-4-dimethylaminoazobenzene.


Investigations on interferon induced by statolon.


The effect of arabinofuranosylcytosine on the growth cycle of simian virus 40.


Attempts to detect an integrated polyoma genome by nucleic acid hybridization II. Complementarity between polyoma virus DNA and normal mouse synthetic RNA.


A single-cell study of chemical inhibition of enterovirus multiplication.


Effect of lymphoid leukosis virus on the response of the chorioallantoic membrane to Rous sarcoma virus.


The inhibition by actinomycin D of poliovirus multiplication HEp 2 cells.


Formation of tumor colonies in the lungs of rats injected with rat embryo cells immediately after infection of the cells with polyoma virus.


Tumor production in the Japanese quail by the Schmidt-Ruppin strain of Rous sarcoma virus.


The inactivation of simian virus 40 infectivity and antigen-inducing capacity by ultraviolet light.


Immunochemical studies on some serological cross-reactions in the Klebsiella group. 10. Structure of the capsular polysaccharide of Klebsiella type 3 (C).


Hepatoma associated with erythrocytosis.


Basal cell nevus syndrome. Combined clinical staff conference at the National Institutes of Health.


On the incorporation of tritium in yeast alcoholdehydrogenase from ethanol-T or NADH-T.


Effect of cortisone on activation of lysosomal enzymes resulting from mengovirus infection of L-929 cells.


Tissue specificity of malate dehydrogenase isozymes. Kinetic discrimination by oxaloacetate and its mono- and difluoro analogues.


Studies on the N-demethylation of morphine and other compounds.


Selective inhibition of nicotinamide adenine dinucleotide dependent oxidation by 2-methyl-3-o-tolyl-4-quinazolone.


Some biochemical effects of sporidesmin.


Glycoproteins as components of the lethal factors in cobra venom (Naja naja).


Studies on biosynthetic enzymes. I. Mutant forms of histidinol dehydrogenase from Neurospora crassa.


Total synthesis of the antibiotic polymyxin B-1.


The stability of angiotensinamide in the presence of urea.


Phosphoproteinphosphatase activity in sea urchin embryos.


The reaction of rabbits to Rous sarcoma virus.


Respiratory virus infections of man and animals. Viruses associated with human respiratory infections.


Respiratory virus infections in man and animals. Some bovine respiratory viruses.


Standardization of the fluorescent antibody technique for the detection of avian respiratory viruses.


Studies on the RIF test for the detection of an avian leukosis virus.


Avian lymphomatosis. IX. Mortality and serological response of chickens of various ages to graded doses of T strain.


Fluorescent-microscopic investigation of cell cultures infected with Virus herpes simplex.


In vitro antiviral activity of hydrazine sulfate.


Adrenocortical activity and peripheral adrenergic responses in the cat.


Sex differences in the effects of abnormal physiological states on the metabolism of drugs by rat liver microsomes.


Study of viral antibodies by the paper-radioactive virus method.


Hormone-secreting tumors of the adrenal cortex in children.


The management of tumours in children.

The management of tumours in children.


Relative target sizes for the inactivation of the transforming and reproductive abilities of polyoma virus.


Radiation target size of the lytic and the transforming ability of polyoma virus.


The nucleic acid of the Bryan strain of Rous sarcoma virus: purification of the virus and isolation of the nucleic acid.


Evidence that a phosphorylated intermediate in a brain transport adenosine triphosphatase is an acyl phosphate.


Angiographic diagnosis of malignant disease of the liver.


Dermatitis as a complication of lymphangiography.


The release of deep pain by nerve injury.


Cell-free transmission of 20-methylcholanthrene-induced RF mouse leukemia, and electron microscopic demonstration of virus particles in the leukemic tissue.


Etiology of upper-respiratory-tract illnesses among civilian adults.


Immunization by selective infection with type 4 adenovirus grown in human diploid tissue culture. II. specific protective effect against epidemic disease.


Genetic factors in breast cancer.

Genetic factors in breast cancer.


The role of viruses in breast cancer causation.


The meaning of biochemical differences between normal and cancer cells.


Effects on central nervous system, peripheral nerve, myoneural junction, and muscle by carcinoma remote from such organs.


Viruses as related to cancer.

Viruses as related to cancer.


Remarks on oncogenic virus-cell relationships.


The inhibitory effect of pyrophosphate on calcium oxalate precipitation and its relation to urolithiasis.


Some remarks about the effect of glycerol on cells during freezing and thawing: electron-spin resonance investigations concerning this effect.


Paramagnetic absorption of irradiated glycine.


Comments on the factors controlling product stereospecificity in the reduction of carbonyl compounds with alcohol dehydrogenase and diphosphopyridine nucleotide.


The isolation and selected properties of blood monocytes. A technique is described for the quantitative recovery of monocytes from horse blood by means of flotation on dense albumin solutions. Monocytes are concentrated in a surface pellicle along with a few lymphocytes which are then removed when the monocytes adhere to a glass surface. The in vitro cultivation of homogeneous populations of monocytes results in an increase in (a) cell size, (b) number of mitochondria, and (c) phase-dense granules of the centrosphere. The phase-dense granules are osmiophilic and acid phosphatase positive. Quantitative biochemical analysis during cultivation have revealed increased levels of cytochrome oxidase, acid phosphatase, arylsulfatase, and BPN hydrolase. In addition, glucose utilization and lactic acid production are stimulated under the same conditions. The uptake of both bacteria and colloidal gold is stimulated during in vitro cultivation. The phagocytic activity of cultured monocytes may be enhanced by a purified bacterial lipopolysaccharide. These data are consistant with the in vitro maturation of monocytes to macrophages, a cell with greater metabolic and functional potentional.


The role of methionine deficiency in poliovirus replication in tissue cultures. The role of methionine in poliovirus infection in HeLa and monkey kidney cells was investigated by using the methionine analogue l-ethionine. In the presence of 2.0 x 10(-3) and 4.0 x 10(-3) moles ethionine, the growth of HeLa and monkey kidney cells was significantly inhibited. Under the same experimental conditions, ethionine had no significant effect on the biosynthesis of two strains of poliovirus (Mahoney and Lansing) in HeLa cells, whereas in primary monkey kidney cells, it markedly inhibited the biosynthesis of the Lansing strain of poliovirus. HeLa cells partly depleted of their intracellular amino acids did not change the rate of viral biosynthesis. The inhibitory effect of ethionine on cell growth and viral biosynthesis was reversed by addition of an excess of l-methionine.


Extracellular products of blue-green algae.


The treatment of multiple sclerosis with ACTH.


Serologic and virus-isolation studies of patients with varicella or herpes-zoster infection.


Antiviral activity of herbs on Columbia SK in mice, and LSM, vaccinia and adeno type 12 viruses in vitro.


Complement fixation: a modified procedure designed to eliminate anticomplementary and procomplementary effects.


Inhibition of renin secretion by angiotensin. II.


Effect of dietary orotic acid on liver glycogen.


Ependymomas produced in Syrian hamsters by adenovirus 7, strain E46 ("hybrid" of adenovirus 7 and SV40).


Lactic dehydrogenase isozymes in rat tissues, tumors, and precancerous livers.


New method of detection of certain bacterial oxidoreductases and transaminases by the indicator reaction on agar.


Studies of avian sarcoma and erythroblastosis (strain 13). IV. pH stability of the virus at 2 degrees Ca.


The connective tissues of peripheral nerves.


The recovery of smallpox virus from patients and their environment in a smallpox hospital. Attempts had been made in 1961 to recover smallpox virus by air sampling in smallpox wards and close to the mouths of smallpox patients, but these had been largely unsuccessful, possibly owing to the air sampling method used. Further attempts were therefore made in 1963, with a fluid impinger for air sampling and with Petri dishes placed below the orifice of the impinger to collect large droplets or particulate matter that the impinger might miss.Air samples from near the patients' mouths yielded little virus, this being more readily recovered from the settling-plates. Patients' bedclothes sampled with the impinger yielded rather more virus, but again even more was obtained from the Petri dishes.The results suggest that contamination of the air in the vicinity of smallpox patients is due to relatively large particles of infected dust from the patients' bedclothes rather than from fine droplets or droplet nuclei coming from the upper respiratory tract. Secretions from the mouth and upper respiratory tract appear to be responsible for the early contamination of pillows and bedclothes.


Injuries to nerves and tendons in the hand and forearm: technique of repair.


Bacterial nucleate and phosphorus partition.


Antibodies for Rous sarcoma virus in fowl, animal, and human populations of East Africa. I. Antibodies in domestic chickens and wildfowl.


Studies on the carcinogenic activity of protein-denaturing agents: hepatocarcinogenicity of dioxane.


Deoxyribonucleic acid synthesis in Escherichia coli infected with some deoxyribonucleic acid polymerase-less mutants of bacteriophage T4.


Incomplete growth of simian virus 40 in African green monkey kidney culture induced by serial undiluted passages.


A comparison of the replication cycles of simian virus 40 in human diploid and African green monkey kidney cells.


Studies on the thymidine kinase activity of mouse embryo cells infected with polyoma virus.


SV40-adenovirus "hybrids": presence of two genotypes and the requirement of their complementation for viral replication.


The lactic dehydrogenases of E. coli.

The lactic dehydrogenases of E. coli.


The fate of the alphah atom of L-lactate in perfused rat liver.


Propanediol metabolism and its relation to lactic acid metabolism.


L(+) lactic acid and the steady state of cellular red/ox-systems.


Cytochrome 559 in the microsomes of the adrenal medulla.


A novel plate assay method for amoebicides.


Contamination of egg-adapted canine distemper vaccine by avian leukosis virus.


Crystal-induced myocarditis and pericarditis.


Porosyringoma. Report of a case.

Porosyringoma. Report of a case.


Electron microscopy of tissue cultures infected with porcine polioencephalomyelitis virus.


The fate of serial heterologously transplanted glioblastoma multiforme in the eye of the guinea pig.


Brain as target organ in Clostridium perfringens exotoxin toxicity.


Studies on menadione reductase of bakers' yeast. II. Inhibition of the enzyme activity by NAD and stimulation of that by p-chloromercuribenzoate.


Effects of urea on the activity and structure of yeast alcohol dehydrogenase.


Evaluation of indices of juxtaglomerular cells and zona glomerulosa in human and experimental cirrhosis.


The effect of angiotensinìi on the "clearance" of radioiodine from the skin in hypertension.


The electrophoretic separation of lactate dehydrogenase isoenzymes and their evaluation in clinical medicine.


Protein abnormalities in neuromuscular diseases. I.


Intraperitoneal hemorrhagic rupture of liver.


Parainfluenza 2 virus: increase in hemagglutinin titer on treatment with Tween-80 and ether.


An orally active hypocholesteremic steroid with reduced uterotrophic effect.


Peripheral arterial and renal venous angiotensin concentration.


Reversible inhibition of DNA virus replication with mithramycin.


Antiviral activity of ammonium picrate in mice.


Effect of crude and purified interferons on the gowth of uninfected cells in culture.


Interferon and uninfected cells.

Interferon and uninfected cells.


Effects of hypophysectomy on lipolytic response of adipose tissue to pituitary hormones.


Purification of hypothalamic corticotrophin-releasing factor (CRF) of ovine origin.


The influence of adrenocorticotropic hormone and prednisolone upon marrow and circulating leukocytes in the dog.


A quantitative study of the infectious bovine rhinotracheitis neutralization test.


Electron microscopy of cytomegalic inclusion disease of swine (inclusion body rhinitis).


Glomus jugularis tumors.

Glomus jugularis tumors.


Amoebiasis in Saskatchewan.

Amoebiasis in Saskatchewan.


Glomus jugulare tumors.

Glomus jugulare tumors.


Antiamebic studies on 5-chloro-793-diethylaminopropyl-aminomethyl)-8-quinolinol in vitro and in experimentally infected animals.


Haemagglutination-inhibiting antibodies to adenovirus types 8, 9 and 10 in paired sera of patients with epidemic keratoconjunctivitis.


Multiple sclerosis in vestfold county.

Multiple sclerosis in vestfold county.


The effect of angiotensin on renal circulation.


Intratypic serodifferentiation of oral poliovaccine progeny.


Path of carbon and assimilatory power in chemosynthetic bacteria. I. Nitrobacter agilis.


The effect of ethanol metabolism on levels on oxidized and reduced nicotinamide-adenine dinucleotide in liver, kidney, and heart.


Changes in fungi with age. II. Respiration and respiratory enzymes of Rhizoctonia solani and Sclerotium bataticola. Van Etten, James L. (University of Illinois, Urbana), H. Peter Molitoris, and David Gottlieb. Changes in fungi with age. II. Respiration and respiratory enzymes of Rhizoctonia solani and Sclerotium bataticola. J. Bacteriol. 91:169-175. 1966.-The rate of respiration of Rhizoctonia solani and Sclerotium bataticola decreased with age. This decrease in respiratory rate might be produced by a decrease in the specific activity of one or more enzymes involved in carbohydrate metabolism. Specific activities in cell-free extracts were measured for most of the enzymes in the hexose monophosphate shunt, Embden-Meyerhof-Parnas pathway, tricarboxylic acid cycle, and terminal electron-transport system. In addition, glucose oxidase, isocitritase, and malic enzyme were measured. In R. solani, increases in activity with age occurred for hexokinase, alpha-glycerolphosphate dehydrogenase, malic dehydrogenase, and cytochrome oxidase. Decreases occurred for phosphohexokinase, aconitase, nicotinamide adenine dinucleotide-specific isocitric dehydrogenase, reduced nicotinamide adenine dinucleotide oxidase, and at least one of the enzymes between 3-phosphoglycerate and pyruvate. In S. bataticola, increases in activity with age were observed for phosphohexokinase, pyruvic dehydrogenase, fumarase, malic dehydrogenase, and malic enzyme, whereas none of the enzymes decreased. The specific activities of the remaining enzymes did not change with age in either fungus.


Inhibition of Mengo virus by interferon. Gauntt, Charles J. (The University of Texas, Austin), and Royce Z. Lockart, Jr. Inhibition of Mengo virus by interferon. J. Bacteriol. 91:176-182. 1966.-The inhibition of Mengo virus replication in L cells resulting from interferon was studied quantitatively. Interferon was titrated on L cells with Western equine encephalomyelitis (WEE) virus as the challenge virus. One protective unit (PU) of interferon is the least amount of interferon which prevents cytopathic effects when a large multiplicity of WEE virus is added subsequent to overnight incubation with interferon. Ten PU of interferon reduced the yields of Mengo virus by about 90%. Larger doses of interferon, up to 220 PU, caused no further reduction in the amount of virus produced. Plaque formation by Mengo virus was also reduced in number by about 85 to 90%, but could not be further reduced. The plaques which formed on interferon-treated cells were reduced in size. We were unable to obtain a virus population with increased resistance to interferon action by use of five successive growth cycles in interferon-treated cultures. Analysis of the cell population for the proportion of cells able to act as infectious centers revealed that incubation of cells with 10 PU of interferon decreased the proportion of virus-yielding cells by 80%. The yield of virus per virus-producing cell was decreased by 40 to 60%. Despite the reduction in yields, plaques, and infectious centers resulting from interferon, all doses of interferon failed to prevent the complete destruction of the cells. Experiments with puromycin indicated that the cytopathic effects observed in L cells infected with Mengo virus required that a virus-directed protein be synthesized between 4 and 5 hr postinfection. The evidence suggested, therefore, that the Mengo virus genome was able to code for new protein synthesis in the absence of the production of infectious virus.


Specific cell-surface alteration by enteroviruses as reflected by viral-attachment interference. Crowell, Richard L. (Hahnemann Medical College, Philadelphia, Pa.). Specific cell-surface alteration by enteroviruses as reflected by viral-attachment interference. J. Bacteriol. 91:198-204. 1966.-Exposure of HeLa cells to high levels of coxsackievirus B3 produced cells which were refractory to attachment of coxsackievirus B1, whereas poliovirus T2 attached normally. Under similar conditions, poliovirus T2 was found to interfere with the attachment of poliovirus T1 to HeLa cells without affecting the attachment rate of coxsackievirus B3. The data confirm earlier findings that the receptor sites on HeLa cells, which bind members of group B coxsackieviruses, are distinct from those for polioviruses. Quantitatively, coxsackieviruses B1 and B3 were found to be mutually exclusive in the attachment interference assay to suggest that they compete for the same receptors on the HeLa cell surface. The finding that input multiplicities of B3 virus which exceeded 500 saturated the homologous viral receptors of HeLa cells was unexpected, but was consistent with the results of interference assays. Excessive amounts of input virus did not, however, inhibit eclipse of homologous cell-associated virus. Attachment interference between enteroviruses occurred even though the interfering virus was eclipsed prior to addition of challenge virus. The finding that enterovirus attachment interference was reversible with acid pH suggested that attachment and eclipse of enterovirus does not result in a permanent alteration of the cell membrane and that these events occur at the cell surface.


Interactions of human cytomegalovirus with human fibroblasts. Vonka, Vladimir (Baylor University College of Medicine, Houston, Tex.), and Matilda Benyesh-Melnick. Interactions of human cytomegalovirus with human fibroblasts. J. Bacteriol. 91:213-220. 1966.-Virus attachment of human cytomegalovirus to human embryo lung fibroblasts was found to be temperature-independent, from 4 to 37 C. Prolonged incubation at 4 C, however, resulted in inactivation of a high proportion of attached virus. Virus penetration seemed to be temperature-dependent, occurring at 37 C but not at 4 C. Detailed studies of the growth curve of the virus were made. Cell-associated virus preceded the appearance of virus in the fluid phase by 2 to 5 days. Complement-fixing antigen could be detected, but only when the cytopathic effect was advanced, and it was demonstrable only in the cell-associated fraction. Under methyl cellulose, decreasing the bicarbonate concentration in the overlay from 0.225 to 0.15% resulted in marked increase in plating efficiency with all strains tested. However, varying the concentration of bicarbonate from 0.3 to 0.15% in fluid medium did not influence the growth of virus.


Thermoinactivation of human cytomegalovirus. Vonka, Vladimir (Baylor University College of Medicine, Houston, Tex.), and Matilda Benyesh-Melnick. Thermoinactivation of human cytomegalovirus. J. Bacteriol. 91:221-226. 1966.-The inactivation at 4 and 37 C of several strains of human cytomegalovirus was studied. The preliminary findings that freshly harvested cytomegalovirus was inactivated more rapidly at 4 C than at higher temperatures was confirmed. Intracellular virus still within infected cells was found to be more stable at 4 C than virus released by sonic treatment just before incubation at 4 C. The composition of the diluent played an important role. In tris(hydroxymethyl)-aminomethane buffer, virus was unstable at both 4 and 37 C, with the rate of inactivation faster at 4 than at 37 C. Similar results were obtained when bicarbonate-phosphate buffer or Eagle's medium when bicarbonate was used as virus diluent. Calf serum stabilized the virus at 37 C, but not at 4 C. The deletion of bicarbonate from Eagle's medium had a stabilizing effect at both temperatures. An even greater stabilizing effect at both 4 and 37 C was obtained when distilled water was used as virus diluent. Inactivation rates varied from one strain to the next at 4 C but not at 37 C. Differences were found also with virus progeny derived from a single strain, but harvested at different stages during virus multiplication. Virus harvested early was more labile at 4 than at 37 C, whereas the late virus was more labile at the higher temperature. Intracellular and extracellular virus preparations were inactivated at the same rates at either 4 or 37 C.


Antiviral action of mouse interferon in heterologous cells. Buckler, Charles E. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), and Samuel Baron. Antiviral action of mouse interferon in heterologous cells. J. Bacteriol. 91:231-235. 1966.-The antiviral action of mouse interferon in cell cultures of mouse, hamster, rat, chicken, and monkey origin was investigated. Using a vesicular stomatitis virus (VSV) plaque reduction test, we found that mouse serum interferon, assayed on closely related rat or hamster cells, exerted 5% of its homologous antiviral activity. This activity was characterized as interferon by its temperature of inactivation, trypsin sensitivity, nonsedimentability, stability at pH 2, lack of inactivation by antibody to virus, and inability to be washed off cells. In the more distantly related chicken and monkey cells, mouse interferon had less than 0.1% of its homologous activity. Conflicting reports of heterologous activity of chicken and mouse interferon preparations may result in part from the observed action of noninterferon inhibitors of vaccinia virus. These inhibitors, like interferon, are stable at pH 2. They are present in mouse serum, mouse lung extracts, and allantoic fluid, and they prevent the development of vaccinia plaques when allowed to remain in contact with cells during virus growth. Unlike interferon the inhibitors are removed by adequate washing of cells prior to virus challenge, and they are not active in the VSV assay system. These findings reemphasize the need for thorough characterization of interferon preparations.


Replication in simian cells of defective viruses in an SV40-adenovirus "hybrid" population. Butel, Janet S. (Baylor University College of Medicine, Houston, Tex.), and Fred Rapp. Replication in simian cells of defective viruses in an SV40-adenovirus "hybrid" population. J. Bacteriol. 91:278-284. 1966.-An SV40-adenovirus type 7 "hybrid" virus population, previously shown to contain two viruses capable of complementation in green monkey kidney (GMK) cells, has a growth cycle in GMK cells similar to that of adenovirus type 7 in the presence of SV40. Extending previous preliminary results, the addition of adenovirus types 2, 7, or 12 to monolayers of GMK cells enhanced plaque formation by the SV40-adenovirus hybrid by as much as 200-fold. The terminal enhanced plaques, initiated by the hybrid in the presence of helper adenovirus, were found to contain progeny which could induce the synthesis of SV40 tumor antigen but which were coated with the protein of the helper adenovirus, type 2, 7, or 12, respectively. The particle carrying the SV40 tumor antigen determinant, named PARA, is defective in that it cannot direct the synthesis of capsid protein; information for the coat for PARA is supplied by the adenovirus. One-step growth curves of the hybrid virus population in monkey cells revealed that synthesis of both types of particles, adenovirus and PARA, proceeds at a similar rate, with a latent period of 16 to 20 hr being followed by an exponential increase in titer during the following 20 hr. Maximal titers for both particles were obtained 48 hr after inoculation of the cultures. Neither the PARA nor the adenovirus component replicated in GMK cells in the absence of the other.


Bacteriophage-induced functions in Escherichia coli K (lambda) infected with rII mutants of bacteriophage T4. Rutberg, Blanka (Karolinska Institutet, Stockholm, Sweden), and Lars Rutberg. Bacteriophage-induced functions in Escherichia coli K(lambda) infected with rII mutants of bacteriophage T4. J. Bacteriol. 91:76-80. 1966.-When Escherichia coli K(lambda) was infected with rII mutants of phage T4, deoxycytidine triphosphatase, one of the phage-induced early enzymes, was produced at initially the same rate as in r(+)-infected cells. Deoxyribonuclease activity was one-third to one-half of that of r(+)-infected cells. This lower deoxyribonuclease activity was observed also in other hosts or when infection was made with rI or rIII mutants. Presence of chloramphenicol did not allow a continued synthesis of phage deoxyribonucleic acid in rII-infected K(lambda). No phage lysozyme was detected nor was any antiphage serum-blocking antigen found in rII-infected K(lambda). It is suggested that the rII gene is of significance for the expression of phage-induced late functions in the host K(lambda).


Purification and properties of phosphodiesterase from bovine pancreas.


Antibody for collagenase from Clostridium histolyticum.


Notes on the role of sulfhydryl and imidazole groups in myosin nucleoside triphosphatase and contraction of glycerinated muscle fibers.


The footprints of tumor viruses.

The footprints of tumor viruses.


Effect of diencephalic and midbrain stimulation on ACTH levels in unrestrained cats.


Genetic and possible biochemical mechanisms in viral carcinogenesis.


The effect of corticosteroids and altered adrenal function on liver regeneration following chemical necrosis and partial hepatectomy.


Plasma enzymes in tumor-bearing rats.

Plasma enzymes in tumor-bearing rats.


Neoplasms following irradiation of the head.


Effects of 6-diazo-5-oxol-norleucine and other tumor inhibitors on the biosynthesis of nicotinamide adenine dinucleotide in mice.


Decontamination of pleuropneumonia-like organism (PPLO) infected tissue cultures.


Role of interferon during viremia. II. Protective action of circulating interferon.


Role of interferon during viremia. I. Production of circulating interferon.


Viruria in herpes simplex infection of mice.


The control of pituitary corticotrophic function.


Breast carcinoma in children.

Breast carcinoma in children.


Hemorrhage in normal man. The effects of angiotensin.


Estimation of plasma renin and renin-substrate in various diseases in man.


Adrenocortical response to high dosage ACTH therapy in patients with multiple sclerosis.


Adsorption of attenuated and neurovirulent strains of poliovirus to central nervous system tissues.


Multifocal pseudohypertrophic neuropathy.


Cellular and antiviral effects of halogenated deoxyribonucleosides.


Antiviral substances. Nucleosides. Discussion.


Inhibition by halogenated deoxyribonucleosides of the production of tumors by a polyoma virus.


Indirect inhibition of protein synthesis.


Experiences in developing potential antiviral compounds.


Direct inhibition of protein synthesis and its effect upon the growth of RNA viruses.


Further studies on the effect of p-fluorophenylalanine and puromycin on polio virus replication.


The use of antiviral compounds in analyzing the sequential steps in the replication of SV40 papovavirus.


Mechanism of cell damage in polio virus infection.


Agents useful in the study of mechanisms of viral replication. Discussion.


Problems of in vitro and in vivo testing of antiviral substances.


Tumorigenic virus and cell responses to biologically active compounds. II. Pharmacologic specificity in cell-virus relationships.


Development of resistance to antiviral drugs.


In vivo and in vitro inhibition of hepatitis viruses by streptothricin.


Antiviral activity of paolins from clams.


The effect of polyanions and polycations on Mengo virus--l cell interaction.


The characteristic and relationships of virus resistance and dependence to guanidine and other compounds.


Studies on the antiviral activity of amantadine hydrochloride.


"Cyclopin".

"Cyclopin".


Virus inhibitory activity of biguanides and related compounds.


Antiviral activities of extracts of the lemon balm plant.


Antiviral activity of some pteridines in animals.


Observations on antiviral screening.

Observations on antiviral screening.


Searching for antiviral materials from microbial fermentations.


Detection and evaluation of potential antiviral drugs. Discussion.


The excretion of steroids by pre-pubertal children during growth.


DNA lesions induced in nuclei and nucleoli of monkey kidney cells in tissue culture by simian vacuolating virus (SV40).


The semiquantitative determination of total and free silica by paper chromatography.


Chromatographic fractionation of antibiotic 136 and characterization of its components.


On feline rhinotracheitis.

On feline rhinotracheitis.


Studies on the pathogenesis of gastroenteritis in swine. Preliminary report.


Epinephrine and insulin effects. II. ACTH and cortisol.


Corticotrophin and corticosteroids in the management of acute and chronic heart block.


Mitochondrial electron transport: effect of age on sensitivity to inhibitors.


The effect of radio-opaque substances on the ciliated epithelium of the trachea of rabbits.


Studies of conformational changes in glyceraldehyde-3-phosphate dehydrogenase accompanying its catalytic action.


Inhibition of the methylation of nicotinamide by chlorpromazine.


Experimental infectious canine hepatitis. I. The pathology of the disease in guinea-pigs.


Electron transfer within and between haemoprotein molecules.


An animal virus with DNA of high guanine + cytosine content which codes for S-RNA.


An unusual location of guineaworm infestation. Report of a case.


Simian virus 40: replication in the presence of specific antiserum and adenovirus 4. Twelve consecuttive passages of simian virus 40, made in the presence of adenovirus 4 and antiserum to simian virus 40, indicated that simian virus 40 would multiply almost indefinitely under these conditions. The adenovirus and the simian virus had previously replicated together in the absence of antiserum. These data support the conclusion that the SV-40 genome is incorporated within the protein coats of adenovirus 4.


Properties of a new type 3 attenuated poliovirus. V. Neurovirulence tests in monkeys after a passage through the human alimentary tract.


Serologic survey of equine rhinopneumonitis virus infection among horses in various countries.


Secondary antibody response of guinea pigs to parainfluenza and mumps viruses.


Influence of adrenal steroids on self-stimulation rates in rats.


Phosphatases of Mycobacterium 607.

Phosphatases of Mycobacterium 607.


Experimental infection of calves with a bovine adenovirus.


Thermal separation of the synthesis of papovavirus SV40 tumor and virus antigens.


Effect of actinomycin D and halogenated deoxyuridines on the replication of simian virus 5.


Renal vascular responses to drugs in experimental nephrosis in rats.


Adrenal cortical function in experimental alcoholism in dogs.


Sensitivity of strain R avian erythroblastosis virus to ether and chloroform.


Blood pressure in female and male rats following ventromedial hypothalamic lesions placed at four different ages.


Deoxyribonuclease I and II activities in liver and spleen of mice experimentally infected by mouse hepatitis virus.


Characteristics of the growth cycles of four simian enteroviruses (SV2, SV6, SV42, SV49).


Relationship between the ultrastructure and cortical activity of the embryonic adrenal gland in the chicken.


Microcirculatory approach to vasopressor therapy in intestinal ischemic (SMA) shock.


Prolonged survival with islet cell carcinoma producing obstructive jaundice.


The effect of structure-disrupting ions on the activity of myosin and other enzymes.


Studies on the identification and origin of ethanol in mammalian tissues.


Metabolism of pipecolic acid in a Pseudomonas species. 3. L-alpha-aminoadipate delta-semialdehyde:nicotinamide adenine dinucleotide oxidoreductase.


The synthesis of teichoic acids. V. Polyglucosylglycerol phosphate and polygalactosylglycerol phosphate.


Tumors of the vulva and vagina. Sweat gland tumors.


Radiotherapy for salivary gland tumours.


The intracellular localization of heme by a fluorescence technique. A new technique for the intracellular localization of minute amounts of heme and hemoproteins is described. The specimen is treated with 1.5 M perchloric acid in the presence of SH groups, followed by ultraviolet light irradiation in a fluorescence microscope. This fixes the proteins in situ and converts the heme to a porphyrin which fluoresces and is readily visualized. With this technique, hemoglobin has been demonstrated in the nuclei of avian erythrocytes, and in the nuclei of human normoblasts at an earlier stage than previously described. In addition, hemoproteins, presumably cytochromes, have been detected in the cytoplasm and nuclei of myelocytes, in thymus lymphocyte nuclei, in chick embryo liver cytoplasm, and in chick embryo somites.


Cushing's syndrome associated with anaplastic carcinoma of the thyroid gland.


Urinary lactic acid dehydrogenase activity in the diagnosis of urologic neoplasms.


Influence of urine on viruses.

Influence of urine on viruses.


The effect of conjugated estrogens and estriol succinate on fibrinolysis: a clinical correlation in patients with uterine bleeding.


Multiple granular-cell myoblastoma of the tongue. Report of a case.


Adrenocortical nuclear volume and adrenocortical function.


Effect of neonatal thymectomy on endocrine and lymphatic organs, reticular elements and blood count. I. Findings in rats not suffering from wasting syndrome.


Rate-limiting factors in glycolysis and transport of inorganic phosphate in DBAH-1 tumor, DBAG tumor, Novikoff hepatoma, and Novikoff ascites tumor.


Serum protein-bound fucose in patients with breast masses.


The incidence of cancer in quantitatively transplanted mammary glands and its relation to age and milk agent of the donor and host mice.


Liver response tests. I. Exploratory studies on glucose 6-phosphatase and other liver enzymes.


Liver response tests. II. Effect of coumarin on glucose 6-phosphate metabolism in rat liver.


A study of the effects of zinc and tin administered orally to mice over a prolonged period.


Cellular physiology of cold- and heat-exposed squirrel monkeys (Saimiri sciurea).


Physiological role of the adrenal medulla in the palmar anhidrotic response to stress.


The role of the thymus in carcinogenesis.


Effect of IDU on DNA synthesis. During corneal wound healing.


A simple and sensitive enzymic method for determination of L(+)-lactic acid.


Separation and detection of organic acids on silica gel.


Histophysiological investigation on the zonation, activity, and mode of secretion of the adrenal gland of the frog, Rana temporaria Linnaeus.


Experimental study of bronchographic media on lung.


Actions of isoniazid on NADH dehydrogenase activity of kidney of tuberculous guinea pigs.


Hyperuricemia induced by drugs.

Hyperuricemia induced by drugs.


The separation and detection of organo phosphate pesticides using diphasic paper chromatography.


An electrophorectic method for the detection and estimation of microgramme quantities of neomycin serum protein solutions.


The association of aniridia, Wilms's tumor, and genital abnormalities.


Analysis of 5-HTP-C-14 and its metabolites.


Viral ribonucleic acid polymerase: chick-embryo cells infected with vesicular stomatitis virus or Rous-associated virus.


Hormonal dependence of actidione (cycloheximide) action.


Intratracheal injection into rats of size-graded silica particles.


Comparative study of the activity of the synthetic corticotropin-beta-1-24.


Herpes simplex virus in ocular, nasal, and oral secretions of patients with herpetic keratitis.


Oxidation and peroxidation.

Oxidation and peroxidation.


Prevention of denervation in Bell's palsy.


On the mechanism of respiratory control. Control of oxidation is the key mechanism in the regulation of energy metabolism. In glycolysis the oxidation of glyceraldehyde-3-phosphate is controlled by DPNH, which inhibits glyceraldehyde-3-phosphate dehydrogenase. In oxidative phosphorylation the inhibition of electron flow from DPNH to oxygen, called "respiratory control," is the subject of this paper. After a discussion of the physiological significance of the "tight coupling" between phosphorylation and oxidation, studies on "loosely coupled" submitochondrial particles are reported. These particles are capable of oxidative phosphorylation in the presence of a suitable phosphate acceptor system, but in contrast to controlled, intact mitochondria they oxidize DPNH in the absence of phosphate and ADP. The addition of o-phenanthroline to submitochondrial particles gives rise to an inhibition of respiration, which is partly reversed by phosphate and ADP or by dinitrophenol. The properties of this model system of respiratory control will be described.


Autologous implantation of rhesus monkey cells "transformed" in vitro by simian virus 40.


Malignant islet cell adenoma in a pancreatic cyst. Report of a case.


Reaction of oxygen with the respiratory chain in cells and tissues. This paper considers the way in which the oxygen reaction described by Dr. Nicholls and the ADP control reactions described by Dr. Racker could cooperate to establish a purposeful metabolic control phenomenon in vivo. This has required an examination of the kinetic properties of the respiratory chain with particular reference to methods for determinations of oxygen affinity (K(m)). The constant parameter for tissue respiration is k(1), the velocity constant for the reaction of oxygen with cytochrome oxidase. Not only is this quantity a constant for a particular tissue or mitochondria; it appears to vary little over a wide range of biological material, and for practical purposes a value of 5 x 10(7) at 25 degrees close to our original value (20) is found to apply with adequate accuracy for calculation of K(m) for mammalia. The quantity which will depend upon the tissue and its metabolic state is the value of K(m) itself, and K(m) may be as large as 0.5 microM and may fall to 0.05 microM or less in resting, controlled, or inhibited states. The control characteristic for ADP may depend upon the electron flux due to the cytochrome chain (40); less ADP is required to activate the slower electron transport at lower temperatures than at higher temperatures. The affinity constants for ADP control appear to be less dependent upon substrate supplied to the system. The balance of ADP and oxygen control in vivo is amply demonstrated experimentally and is dependent on the oxygen concentration as follows. In the presence of excess oxygen, control may be due to the ADP or phosphate (or substrate), and the kinetics of oxygen utilization will be independent of the oxygen concentration. As the oxygen concentration is diminished, hemoglobin becomes disoxygenated, deep gradients of oxygen concentration develop in the tissue, and eventually cytochrome oxidase becomes partially and then completely reduced. DPN at this point will become reduced and the electron flow diminished. The rate of ATP production falls and energy conservation previously under the control of the ADP concentration will now be controlled by the diffusion of oxygen to the respiratory enzymes in the mitochondria. Under these conditions the rate of reaction of cytochrome oxidase with oxygen and the reaction of cytochromes with one another become of key importance. The rise of ADP and the depletion of energy reserves evoke glycolytic activity, and failure of biological function may result.


Copper proteins and oxygen. Correlations between structure and function of the copper oxidases. A comprehensive survey of the interaction of the copper proteins and oxygen is presented including a correlation of structure, function, and other properties of the known copper oxidases and of hemocyanin. The origin of their blue color and the structure of copper complexes and copper proteins are related to the oxidation state of copper ion and relevant electronic transitions probably arising from the formation of charge transfer complexes. The oxygen reactions of hemocyanin, ceruloplasmin, and cytochrome oxidase show half-saturation values far below the other Cu enzymes. The formation of hydrogen peroxide as a reaction product is associated with the presence of one Cu atom per oxidase molecule or catalytic system. Water is the corresponding product of the other Cu oxidases with four or more Cu atoms per molecule, except for monoamine oxidase. Mechanisms for the oxidase action of the two and four electron transfer Cu oxidases and tyrosinase are proposed. These reactions account for the number, the oxidation-reduction potential, and the oxidation state of Cu in the resting enzyme, the cyclical change from Cu(II) to Cu(I), the diatomic nature of O(2), the sequence of the oxidation and reduction reactions, and other salient features. The catalytic reactions involved in the oxidation of ascorbic acid by plant ascorbate oxidase, ceruloplasmin, and Cu(II) are compared. Finally the substrate specificity, inhibitory control, and the detailed mechanism of the oxidase activity of ceruloplasmin are summarized.


Enzymic studies on the mechanism of double hydroxylation.


Oxy-coenzyme A: a competitive inhibitor of coenzyme A in the phosphotransacetylase reaction.


Isolation of different-sized substrains from three stock cultures of Entamoeba histolytica, with observations on spontaneous size changes affecting whole populations.


Free amino acid analyses of 4 strains of Entamoeba histolytica and of E. invadens in the CLG medium.


Bilateral mixed tumor of the parotid initially regarded as pharyngeal neoplasm.


Case records of the Massachusetts General Hospital. Case 10-1966.


The pathogenicity of the small race of Entamoeba histolytica to splenectomized rats.


An assay for cellular transformation by SV40.


Structure of virus particles, partially purified from the blood of leukemic mice.


Antiviral activity of gliotoxin.

Antiviral activity of gliotoxin.


Anaphylaxis in pregnant and mother mice following active and passive sensitization.


Bovine enteric cytopathogenic viruses. 3. Neutralization studies with animal sera.


An indirect hemagglutination test for detecting antibody to infectious bovine rhinotracheitis virus.


Variants of myxovirus parainfluenza-3 isolated from calves clinically affected with shipping fever.


Treatment of Pseudomonas keratitis in rabbits.


Histologic changes in clinically normal breasts at postmortem examination.


Hormonal induction of ceruloplasmin in chicken serum.


Antigenic comparison of two substrains of Entamoeba histolytica by gel diffusion and immunoelectrophoresis.


Non-specific hemadsorption by rhesus monkey kidney cells.


A new virus isolated from the human respiratory tract.


Neutralizing antibodies to simian virus 40 (SV40) in human sera from India.


The mobilization of fatty acids from adipose tissue by pituitary peptides and catechol amines.


Effect of glucose on spontaneous limitation of lipolysis in isolated adipose tissue: a potential regulatory mechanism.


Acute effects of corticotropin and related peptides on carbohydrate and lipid metabolism.


Effect of ACTH on FFA release and diglyceride content in perfused rat adipose tissue.


Modulation of lipolysis in adipose tissue by fatty acid concentration in fat cell.


Seasonal variations in calcium, inorganic phosphate and alkaline phosphatase contents of Ophicephalus punctatus Bloch.


Physiologic and morphologic microcirculatory responses in humans.


Pathogenicity of the M and E variants of the encephalomyocarditis (EMC) virus. I. Myocardiotropic and neurotropic properties.


The nature and control of infections in patients with acute leukemia.


Urinary cortisol metabolites in adrenocortical hyperfunction.


The conservative surgical treatment of arrhenoblastoma. Report of a case.


Influence of collagenase on collagen metabolism in IF mice.


Polymannoheptose isolated from cell wall of uridine diphosphate glucose pyrophosphorylase-less mutant of Escherichia coli K 12.


Effects of neonatal thymectomy on polyoma tumor formation.


Cloacogenic anorectal carcinoma.

Cloacogenic anorectal carcinoma.


The effect of mechlorethamine and cyclophosphamide on the nicotinamide-adenine dinucleotide glycohydrolase activity in Ehrlich ascites cells and L1210 leukemia cells.


Colitis cystica profunda.

Colitis cystica profunda.


Histochemical demonstration of the soluble enzyme, ketose reductase (sorbitol dehydrogenase), by utilizing a new fixing solution.


Pleural calcification as a sign of silicatosis.


A new method for the determination of deoxyribonucleic acid.


Different pattern of elution of poliovirus strains from DEAE-cellulose and aluminium hydroxide gel.


A new method for the determination of cyanhydric acid and cyanides in the air and in biologic products.


Ionic effects on glutamate dehydrogenase activity from beef liver, lobster muscle and crab muscle.


The correlations between multiple congenital malformations and the cytomegalic inclusion disease.


Relationship between the pituitary gland and the corticosteroid-binding globulin in the rat.


Effect of ACTH on cardiac glycogen.

Effect of ACTH on cardiac glycogen.


Effects of 1-methyl-2-mercaptoimidazole and perchlorate on the insulin-mediated enhancement of 131-I incorporation into iodoamino acids by fetal thyroid glands in organ culture.


Association of adrenocorticotropic hormone activity with small secretory granules from rat anterior pituitary glands.


Cystosarcoma phyllodes.

Cystosarcoma phyllodes.


Bilateral Wilms' tumor, including report of a patient surviving ten years after treatment.


Apparent bone age in cancer.

Apparent bone age in cancer.


2-keto-3-deoxygalactonic acid as a constituent of an extra-cellular polysaccharide of Azotobacter vinelandii.


Direct irradiation of lymph nodes with I-131 tagged ethiodol.


On the stimulation of testicular steroidogenesis in the rabbit by interstitial cell-stimulating hormone.


Antilipolytic effect of insulin in isolated fat cells of the rat.


Adrenocortical response to stress and ACTH in Pacific salmon (Oncorhynchus tshawytscha) and steelhead trout (Salmo gairdnerii) at successive stages in the sexual cycle.


Induction of permanent diabetes in rats by pituitary hormones from a transplantable mammotropic tumor. Concomitant changes in organ weights and the effect of adrenalectomy.


Chemotherapy of malignant brain tumors.

Chemotherapy of malignant brain tumors.


Angiotensin sensitivity in experimental uteroplacental ischemia.


Teaching adolescent management in a state hospital.


Liver glucose-6-phosphatase and pyrophosphate-glucose phosphotransferase: effects of fasting.


The effect of angiotensin II on the sensitivity of the parotid gland of the sheep to aldosterone.


Just start with a little T. L. C.

Just start with a little T. L. C.


A non-traditional role of the nurse: the psychiatric nurse in the community.


Nursing dress, an experimental evaluation of its effect on psychiatric patients.


Nicotinic acid biosynthesis in prototrophs and tryptophan auxotrophs of Saccharomyces cerevisiae.


Beef heart malic dehydrogenases. IV. Fluorescent binary and ternary complexes of the supernatant enzyme.


An American nurse's experience in a therapeutic community in Scotland.


Beef heart malic dehydrogenases. V. A kinetic study of the reaction catalyzed by the supernatant enzyme.


Role playing as a device to assist long-term patients nearing discharge.


Dry skin and its treatment.

Dry skin and its treatment.


International trends. Organization of psychiatric rehabilitation in Poland.


Stimulation of metabolism of rat brown adipose tissue by addition of lipolytic hormones in vitro.


The thirteenth stair.

The thirteenth stair.


Geriatrics.

Geriatrics.


Investigation of myosin heterogeneity observed during chromatography on diethylaminoethyl cellulose.


Biosynthesis of mannophospholipids by Mycobacterium phlei.


Studies on cytochrome oxidase. II. A reaction of cyanide with cytochrome oxidase in soluble and particulate forms.


Biological mechanisms involved in the formation of deoxysugars. 3. Enzymatic conversion of thymidine diphosphoglucose-3T to thymidine diphospho-L-rhamnose.


The metabolism of coumarin by a microorganism. V. Melilotate hydroxylase.


The chemistry of lipids of posthemolytic residue or stroma of erythrocytes. XIV. Chemical structure of glycolipid of cat erythrocyte stroma.


Structure of a rhamnolipid from Pseudomonas aeruginosa.


Hypoglycemia due to obstruction of pancreatic excretory ducts by carcinoma.


Functional islet tumor cured by surgical excision.


Clinical applications of nerve conduction velocity.


The effect of hydrocortisone and x-irradiation on the lymphocytosis induced by Bordetella pertussis. 1. In mice rendered lymphocytopenic by X-irradiation or hydrocortisone acetate, pertussis vaccine evoked both lymphocytosis and polymorphonuclear leukocytosis. 2. When mice with lymphocytosis induced by pertussis vaccine were X-irradiated, prompt and extensive destruction of circulating as well as tissue small lymphocytes occurred. The devitalized circulating cells were cleared from the blood primarily by the Kupffer's cells of the hepatic sinusoids. 3. Hydrocortisone acetate administered to mice with lymphocytosis did not cause acute lymphopenia nor was there any evidence of destruction of circulating small lymphocytes. However, destruction of these cells within lymphoid tissues was apparent. These observations suggested that adrenal cortical hormones are not "lymphocytolytic" with respect to circulating lymphocytes.


Glucose and glycogen metabolism in the human epidermis.


The influence of certain hormones and chemicals on mammalian pigment cells.


Carbohydrate metabolism in psoriasis: an enzymatic study.


Orthostatic hypotension.

Orthostatic hypotension.


Acromegaly pre- and postpituitary irradiation.


A diabetogenic polypeptide from bovine adenohypophysis similar to that excreted in lipoatrophic diabetes.


Studies of adrenocortical function of women with hirsutism: response to 5 units of ACTH.


Functioning neural tumors.

Functioning neural tumors.


Insulin-secreting adenoma of the pancreas.


The preparation of 1-O-tosyl-(--)-inositol from quebrachitol.


Studies on the acetoacetate-like compound found in dystrophic mouse muscle homogenates.


Pyridine nucleotide oxidizing enzymes of Lactobacillus casei. I. Diaphorase.


Pyridine nucleotide oxidizing enzymes of Lactobacillus casei. II. Oxidase and peroxidase.


Phosphopeptides: synthesis and enzymic studies.


Liver alcohol dehydrogenase as a 3-beta-hydroxy-5-beta-cholanic acid dehydrogenase.


Chemical synthesis of N-acetylgalactosamine-alpha I-phosphate 6-O-sulphate, N-acetylchondrosine sulphate and UDP-N-acetylgalactosamine 6-O-sulphate.


Studies on the structure of neuramin-lactose sulfate.


The dissolution and fractionation of bovine sclera.


The production of hyaluronate by spontaneously established cell lines and viral transformed lines of fibroblastic origin.


Changes in the activity of rat adrenal ribonuclease and ribonuclease inhibitor after administration of corticotrophin.


Characterization of HeLa cell and viral ribonucleic acid by oligonucleotide analysis.


"B" virus disease in monkey and man.

"B" virus disease in monkey and man.


Retention by membrane filters of phosphatase-produced deoxyadenosine in DNA polymerase assay.


Myocardial function (studied using angiotensin) after aortic homograft valve replacement.


Heterogeneity of liver alcohol dehydrogenase on starch-gel electrophoresis. 1. Purified horse-liver alcohol dehydrogenase is heterogeneous on starch-gel electrophoresis in several buffer systems. 2. The electrophoretic pattern is altered by the addition to the buffers of oxidized or reduced coenzymes, isobutyramide, metal ions or metal-chelating agents. 3. The effect of coenzymes on the pattern suggests that the major cause of the observed heterogeneity is not the existence of isoenzymes, but the presence in the enzyme preparations of coenzyme-enzyme complexes or complexes with other nucleotides similar to, but less reactive than, the coenzymes. 4. Metal ions and chelating agents influence the electrophoretic separation by partial denaturation and inactivation of the enzyme.


The purification and properties of the alanyl-transfer ribonucleic acid synthetase of tomato roots. 1. The alanyl-s-RNA synthetase of tomato roots has been purified by ammonium sulphate precipitation, adsorption on calcium phosphate gel and DEAE-cellulose chromatography and its properties have been investigated. 2. Enzyme activity was measured by using the hydroxamate assay, the [(32)P]pyrophosphate-ATP-exchange assay and the [(14)C]alanyl-s-RNA assay. The purified enzyme was specific for l-alanine and was activated by Mg(2+) ions and to a smaller extent by Co(2+) and Mn(2+) ions. It was free from adenosine triphosphatase, pyrophosphatase and ribonuclease, and possessed a specific activity comparable with that of the most highly purified aminoacyl-s-RNA synthetases from animal and microbial systems. 3. The properties of the purified enzyme were similar in many respects to most other highly purified aminoacyl-s-RNA synthetases. It differed, however, in that the pH optimum of the hydroxamate assay was almost the same as that of the pyrophosphate-ATP-exchange assay and in requiring a high concentration of l-alanine for maximum activity (100mumoles/ml.). 4. The purified enzyme was not absolutely specific for tomato-root s-RNA; slight activity was also observed with yeast s-RNA. 5. The properties of this enzyme are fully consistent with the suggestion that the enzymic formation of alanyl-s-RNA proceeds via the intermediate formation of alanyl acyl-adenylate with the elimination of pyrophosphate from ATP. It remains to be shown the extent to which alanyl-s-RNA participates further in subsequent stages of protein synthesis in plants.


Diphosphoinositide and triphosphoinositide in animal tissues. Extraction, estimation and changes post mortem. 1. A method is presented for the determination of the di- and tri-phosphoinositide in animal tissues. 2. The polyphosphoinositides are quantitatively extracted into chloroform-methanol-hydrochloric acid solvent after a preliminary chloroform-methanol (1:1, v/v) extraction to remove the bulk of the other phospholipids. On washing this extract with n-hydrochloric acid the polyphosphoinositides pass completely into the lower chloroform-rich phase. Their concentrations in the lower phase are determined by chromatography on formaldehyde-treated paper or chromatography and ionophoresis of the acid hydrolysis products. 3. When guinea-pig brain is extracted by the method of Folch (1942), considerable hydrolysis of the triphosphoinositide and accumulation of diphosphoinositide occurs during the initial acetone extraction. 4. The tri- and di-phosphoinositide contents of rat and guinea-pig brain decline substantially within a few minutes after death. 5. The concentrations of tri- and di-phosphoinositide in rat brain are not changed by insulin-hypoglycaemia or electrical stimulation. 6. Examination of frozen rat tissues showed that the brain contained the highest concentration of polyphosphoinositides. Much smaller amounts are present in kidney, and only trace quantities in liver and lung. None could be detected in spleen, heart and skeletal muscle.


Polyphosphoinositides in myelin.

Polyphosphoinositides in myelin. 1. On fractionation of guinea-pig forebrain homogenates by differential and gradient-density centrifugation most of the polyphosphoinositides were recovered in the myelin-rich particles. 2. The phospholipids of pure preparations of myelin contained di- and tri-phosphoinositide in proportions 2-3 times greater than in the whole-brain phospholipids. 3. Di- and tri-phosphoinositide appeared in young rat brain during the period of myelination. 4. After the administration of [(32)P]phosphate to guinea pigs the labelling of the polyphosphoinositides in isolated pure myelin was as great as in the whole brain, whereas little synthesis of the other myelin phospholipids had occurred. 5. When brain subcellular fractions were incubated with [gamma-(32)P]ATP, some triphosphoinositide labelling occurred in the myelin-rich fraction whereas the active labelling of diphosphoinositide was localized mainly in the mitochondrial fraction. 6. The Na(+), K(+) and Mg(2+) plus Ca(2+) concentrations in purified myelin have been determined. The Mg(2+) plus Ca(2+) content present showed close acid-base equivalence to the polyphosphoinositides. 7. It is concluded that di- and tri-phosphoinositide are rapidly-metabolizing components of the myelin sheath or intimately associated structures.


The purification and properties of malonic semialdehyde oxidative decarboxylase from Prototheca zopfii. 1. An enzyme, which in the presence of NAD(+) and CoA oxidizes malonic semialdehyde to acetyl-CoA, has been purified from an extract of the colourless alga Prototheca zopfii. 2. The purified enzyme has optimum pH7.5, is specific for NAD(+) and requires a thiol compound for maximum activity. 3. The enzyme is inhibited by arsenite, N-ethylmaleimide and urea. 4. The results are discussed in relation to those obtained by other workers with a similar bacterial enzyme, and a possible reaction sequence is proposed.


The mechanism of the periodate-thiobarbituric acid reaction of sialic acids. 1. The chromogen formation from N-acetylneuraminic acid in the periodate-thiobarbituric acid reaction was investigated. Measurement of periodate consumption showed an uptake of approx. 3moles/mole of substrate in neutral as well as in strongly acidic solution. Therefore the chromogen beta-formylpyruvic acid is not a direct product of the periodate oxidation; it is presumed to be formed from the true oxidation product, a hexos-5-uluronic acid, by aldol splitting during the reaction in hot acidic solution with thiobarbituric acid. 2. Methyl (methyl beta-l-threo-hexos-4-enepyranosid)uronate, an analogue of the pre-chromogen, has been shown to yield with thiobarbituric acid in acidic solution a pigment exhibiting an identical absorption spectrum and showing the same behaviour on paper chromatography as the pigment obtained from N-acetylneuraminic acid in the periodate-thiobarbituric acid assay. 3. The substitution at C-2 of methoxyneuraminic acid does not inhibit the periodate-thiobarbituric acid reaction. In neutral solution methoxyneuraminic acid is oxidized by periodate to a substance that reacts readily with thiobarbituric acid in acidic solution. When periodate oxidation is attempted in acidic solution, protonation of the amino group protects this group against oxidation, rendering methoxyneuraminic acid negative in the assay systems of Warren (1959a,b) and Aminoff (1959, 1961).


Nicotinamide nucleotides in the erythrocytes of patients suffering from pellagra. 1. The nicotinamide nucleotide concentrations in the erythrocytes of subjects suffering from pellagra (pellagrins) were not lower than those in normal subjects, but the ability of erythrocytes to synthesize these nucleotides in vitro was significantly lower in pellagrins. 2. The oral administration of 10g. of l-leucine daily for 5 days depressed the nicotinamide nucleotide-synthesizing ability of erythrocytes both in normal subjects and in pellagrins. This was not accompanied by changes in the nucleotide concentration in erythrocytes. 3. Quinolinic acid brought about a significant inhibition of the synthesis of nicotinamide nucleotides in vitro. Such inhibition was partially overcome by increasing the concentration of nicotinic acid in the medium.


Spectral and biological changes induced in nicotinic acid and related compounds by ultraviolet light. 1. Irradiation of nicotinic acid, nicotinamide, nicotinamide N-oxide, N'-methyl-4-pyridone-3-carboxamide, reduced nicotinamide-adenine dinucleotide and pyridine with ultraviolet light at 253.7mmu leads to striking spectral changes. 2. Nicotinic acid and nicotinamide are broken down to photosensitive intermediates which in turn undergo photodecomposition. 3. A major photoproduct of [7-(14)C]nicotinic acid is radioactive and absorbs ultraviolet light, but is inactive as a growth factor for Candida pseudotropicalis. 4. Irradiation of nicotinamide gives rise to small quantities of a biologically active photoproduct having the same R(F) as nicotinic acid. A second photoproduct is also formed, but its identity has not yet been established. 5. Irradiation of nicotinamide N-oxide leads to the formation of several photoproducts, one of which has the same R(F) as nicotinamide, absorbs ultraviolet light, and is biologically active. 6. Evidence is presented that irradiation of ethanolic solutions of N'-methyl-4-pyridone-3-carboxamide gives rise to acetaldehyde. 7. Irradiation of reduced nicotinamide-adenine dinucleotide in the presence of acetaldehyde leads to the formation of oxidized nicotinamide-adenine dinucleotide, which in turn can break down to nucleotide and/or nucleoside (depending on the conditions of the reaction). 8. The quantum yields of photolysis and the molar photosensitivities have been determined for N'-methyl-4-pyridone-3-carboxamide and nicotinamide N-oxide. 9. The possible biological significance of these photoreactions is discussed in relation to photosynthesis, visual-pigment metabolism and ultraviolet-light-induced cell damage. 10. A four-step theory is presented for the biochemical evolution of oxidation-reduction systems, involving photoactivated transformations of pyridine derivatives.


Isolation and characterization of collagenases I and II from Clostridium histolyticum.


Lysosomes in rat-kidney tissue.

Lysosomes in rat-kidney tissue.


The effect of cyanide on the Keiling-Hartree preparation and purified cytochrome oxidase.


WHO collaborative studies on enterovirus reference antisera. This paper summarizes the results of co-operative studies undertaken by the WHO International Reference Centre for Enteroviruses and a number of WHO Regional Reference Centres for viruses, WHO Virus Collaborating Laboratories, or other laboratories in a comprehensive testing programme of enterovirus equine antisera. The studies were designed to appraise the specificity of immune serum prepared in horses against five representative prototype enteroviruses (poliovirus 1, coxsackieviruses A9 and B3, and echoviruses 4 and 11). Tests for neutralizing antibody were performed not only against the homologous viruses, but also against a battery of heterotypic picornaviruses, comprising 63 enteroviruses, several rhinoviruses, and three reoviruses. Each serum sample represented a pool of the individual bleedings taken from groups of five or six horses before and after immunization with each virus antigen (except echovirus 4, for which three animals were immunized). The results showed that, whereas the homologous titres of the antisera (poliovirus 1, coxsackieviruses A9 and B3, echoviruses 4 and 11) were found to be within a useful range (700-8000), heterotypic antibodies of significant titre were not detected. All corresponding pre-inoculation sera were negative. The specificity of the haemagglutination-inhibiting antibody of the coxsackievirus B3 and echovirus 11 antisera was also demonstrated. This accumulated information played a major role in a decision to prepare in horses antisera to the rest of the enterovirus group. Co-operative testing of the other enterovirus equine antisera is now in progress.


Childhood cancer in twins.

Childhood cancer in twins.


Synovial sarcoma. A review of 58 cases.

Synovial sarcoma. A review of 58 cases.


Some epidemiological features of lung cancer among Jewish males.


Melanotic neuroectodermal tumor of infancy--a neoplasm of neural crese origin. Report of a case associated with high urinary excretion of vanilmandelic acid.


Lesions in the temporal bone causing multiple nerve damage.


Kinetic studies on the breakdown of adenosine diphosphate in human plasma.


Glomus tumor (chemodectoma) of the larynx.


Effect of ACTH on the hypothalamic neurosecretion of the pigeon (Columba livia domestica L.).


Comparative study on the lactic acid and hexosamine content as well as on the glycolytic and cytochrome oxidase activities of some lower animals.


Acquired generalized anhidrosis.

Acquired generalized anhidrosis.


Malignant disease of the parotid.

Malignant disease of the parotid.


Studies of an alveolar soft tissue sarcoma.


The histogenesis of mammary tumours induced in the rat by chemical carcinogens. 126 Wistar female rats were given carcinogenic compounds, 2-acetylaminofluorene (2-AAF), 20-methylcholanthrene (MC) and 7,12-dimethylbenz(a)anthracene (DMBA), for induction of breast tumors to determine the site of origin and biological behavior of mammary carcinoma in the rat.  18 virgin rats served as control material of a corresponding age to the tumor-bearing rats.  A dissecting microscope was used to identify early neoplastic lesions in the breast glands for histological study.  17 weeks after carcinogen treatment, breast gland tumors, especially adenocarcinomas became evident by palpation.  Mammary carcinoma was found to be multifocal in origin, arising almost exclusively in ducts, ductules, and end-buds without the intervention of any recognizable lesion preceding the appearance of tumor cells.  Fibroadenomas and adenomas occurred in rats with generally well-developed breast glands, while mammary carcinomas occurred in younger rats with less developed breast glands.  For successful tumor induction, the important factors to consider are the architectural state of the mammary gland, the action of hormonal factors, genetically-determined susceptibility, and the carcinogenic agent and its dosage.  Mast cells did not play a significant role in tumor formation, nor were significant myoepithelial cell contributions found in the early carcinomas and fibroadenomas.


Protection of packaged food from insect infestation by the use of silica gel.


Catecholamines and hemodynamic changes in hypertension.


On the role of renin-angiotensin system with special relation to aldosterone for the etiology of hypertension.