Trophoblastic disease. Concepts of management. Trophoblastic tumors are a notoriously unpredictable group in which metastasis may occur from histologically benign tumors and cause death, whereas a histologically malignant tumor may spontaneously disappear. Additional problems are created by the inability to accurately establish a diagnosis before abortion of molar tissue or development of metastasis.In recent years chemotherapy has dramatically improved the prognosis of patients with choriocarcinoma. This fact, plus the importance of the time interval between onset of disease and the beginning of chemotherapy, makes close follow-up of patients with trophoblastic tumors imperative.Concepts of therapy for trophoblastic tumors are rapidly changing. Indications for chemotherapy are broadening, especially in the areas of persistent and metastatic trophoblastic disease. As surgical intervention in the form of hysterectomy is becoming less important, the reproductive capacity of these young women is being preserved. Astute diagnosis, appropriate therapy and active life-long follow-up are the essentials in the management of patients with trophoblastic disease.


Benzothiadiazine suppression of insulin release from normal and abnormal islet tissue in man.


Specific transplantation immunity in relation to Rous sarcoma virus tumorigenesis in mice. Tests for transplantation immunity and for the occurrence of virus-neutralizing serum antibodies were performed on mice, inoculated when newborn with the Schmidt-Ruppin strain of Rous sarcoma virus (RSV-SR). Mice developing no palpable primary sarcomas showed a clear-cut resistance against the isografting of established specifically antigenic Rous tumors. Transplantation tests performed on primary tumor hosts after extirpation of the tumors revealed neither any clear-cut immunity nor tolerance to the specific transplantation antigen(s). Serial pretreatment of operated primary tumor animals with irradiated autologous or syngeneic tumor cells resulted in a clear-cut transplantation immunity. Virus-neutralizing activity was only found in a few sera from newborn infected mice, and in these cases there was no positive correlation with the transplantation immunity. It seems probable that a successful immunization against the RSV-SR specific transplantation antigen(s) prevents the development of primary tumors. There is no indication of any tolerance to this antigen in connection with the induction of primary tumors.


The effects of combined renal vasodilatation and pressor agents on renal hemodynamics and the tubular reabsorption of sodium.


D(-)-lactate dehydrogenase in lower fungi. A lactate dehydrogenase, specific for nicotinamide-adenine dinucleotide and D(-)-lactate, has been detected in extracts from two fungi of the order Leptomitales (Oomycetes). Several fungi of this order carry out a lactic acid fermentation under conditions of reduced oxygen tension.


Mast cells and necrosis.

Mast cells and necrosis. Subcutaneous injections of normally well tolerated amounts of hypertonic NaCl or urea solutions produce extensive topical necroses in rats systemically treated with various mast-cell dischargers and mast-cell products. This response is considered to be closely related to mitast-cell function, for it cannot be duplicated by systemic treatment with a variety of other agents.


Puromycin analogs: action of adrenocorticotropic hormone and the role of glycogen. The effect of the injection into rats of analogs of puromycin, 6-dimethylaminopurine, and the aminonucleoside of puromycin on the stimullation of steroidogenesis by adrenocorticotropic hormone was coin pared with that of puromycin and cycloheximide. This stimulation was blocked only by the antibiotics, which also inhibited adrenal protein synthesis. Glycogenolysis is not associated with the primary mechanism of the adrenocorticotropic hormone stimnulation of steroid hormone biosynthesis in rats.


Chemotherapy in women with trophoblastic disease: choriocarcinoma, chorioadenoma destruens, and complicated hydatidiform mole.


A study of cystosarcoma phyllodes.

A study of cystosarcoma phyllodes.


Unusual tumors of the stomach.

Unusual tumors of the stomach.


Peripheral neuropathy in juvenile diabetes.


Observations on pathogenicity and immunogenicity of a cell-culture-modified foot-and-mouth disease virus.


Primary carcinoma of the liver in Alberta. A survey was made to determine the incidence and to elucidate the manifestations of primary carcinoma of the liver in Alberta. The findings were compared with other reported series. Ninety-six cases were identified: 69 hepatomas, 25 cholangiomas and two cholangiohepatomas. Seventy-four of the patients were male and 22 were female, a male preponderance of greater than 3:1. Ages ranged from 7 days to 92 years, but the majority of the patients (58%) were in the seventh and eighth decades. The incidence of associated cirrhosis (38.5%) was lower than that noted in most series.Hepatomegaly, abdominal pain, weight loss and ascites were the outstanding clinical features. Gastrointestinal hemorrhage was frequent and second only to hepatic failure as the immediate cause of death. An abdominal mass and pleural effusion occurred in higher frequency than that cited in the literature. Associated disorders included peptic ulceration and cholelithiasis.Surgical biopsy was superior to needle biopsy in establishing the diagnosis. Laboratory tests and routine radiographs may be of diagnostic aid.


Detoxified bacterial endotoxns. II. Preparation and biological properties of chemically modified crude endotoxins from Salmonella typhimurium. Martin, William J. (University of Utah, Salt Lake City), and Stanley Marcus. Detoxified bacterial endotoxins. II. Preparation and biological properties of chemically modified crude endotoxins from Salmonella typhimurium. J. Bacteriol. 91:1750-1758. 1966.-Chemical modification of a crude endotoxin prepared by the Roschka-Edwards (RE) procedure from a strain of Salmonella typhimurium yielded products which were nontoxic for mice and had reduced fever effects in rabbits. A reduction in rabbit pyrogenicity of approximately 100 times was noted with a potassium periodate-treated RE preparation when compared with the parent RE preparation. Measured in a similar fashion, pyrogenicity of a potassium methylate-treated RE preparation was reduced by a factor of 10 while pyrogenicity of a boron trifluoride RE preparation was unchanged. All of these endotoxoids, including the parent RE preparation, showed little toxicity for mice. Immunogenicity was determined in mice by comparing Boivin, RE, and endotoxoid preparations with a heat-killed, phenol-preserved (HP) vaccine prepared from the same strain of S. typhimurium. Employing a 10 ld(50) challenge, the protective immunogenicity of the respective vaccines was determined by active immunized mouse protection tests. Although two 100 mug immunizing doses of the Boivin, RE, and the respective endotoxoid preparations varied in mouse protection (potassium methylate RE > Boivin > RE > boron trifluoride RE > potassium periodate RE), it was evident that, with the exception of the potassium methylate preparation, the HP vaccine yielded greatest protection against the 10 ld(50) challenge with S. typhimurium. Further mouse protection experiments suggested that the minimal immunogenic dose of the potassium methylate RE vaccine preparation was approximately 50 mug. These data indicated an approximate fivefold difference between the minimal pyrogenic dose (10 mug) and the minimal immunogenic dose (50 mug). These findings further suggest that potassium methylate RE vaccine preparations should be considered in the search for less toxic enteric fever vaccines.


Bordetella pertussis culture having only species factor 1. Eldering, Grace (Michigan Department of Public Health, Grand Rapids), Jack Holwerda, and Julia Baker. Bordetella pertussis culture having only species factor 1. J. Bacteriol. 91:1759-1762. 1966.-Preston's description of Bordetella pertussis 353-Z as a culture possessing only factor 1 was confirmed by agglutination and agglutinin-adsorption tests. The ld(50) values of this culture for mice inoculated by the intracerebral route were approximately the same as that of B. pertussis 18-323, the challenge culture in mouse potency tests of pertussis vaccine. In mouse protection tests, vaccine prepared with the factor 1 culture protected as well against challenge with B. pertussis 18-323, which has a broad antigenic pattern, as did vaccines made with cultures possessing factors 2, 3, 4, and 5 in addition to factor 1. When two groups of similarly immunized mice were challenged with 353-Z and 18-323, respectively, much lower ed(50) values were obtained with the animals challenged with 353-Z. This was true whether a factor 1 vaccine or a vaccine with factors 1, 2, 3, 4, and 5 was tested.


Immunochemical study of parainfluenza virus (type 2) in amnion cells. de Vaux St. Cyr, C. Columbia University, New York, N.Y.),and C. Howe. Immunochemical study of parainfluenza virus (type 2) in amnion cells. J. Bacteriol. 91:1911-1916. 1966.-Immunoelectrophoretic analysis of stable amnion cells in which parainfluenza virus (type 2) was being actively synthesized revealed at least three precipitating antigens not found in normal cells. These "new" antigens differed from viral neuraminidase and hemagglutinin in both specificity and electrophoretic mobility; their identity and function remain to be elucidated.


Plaque enhancement of enteroviruses by magnesium chloride, cysteine, and pancreatin. Wallis, Craig (Baylor University College of Medicine, Houston, Tex.), Fred Morales, Joycelyn Powell, and Joseph L. Melnick. Plaque enhancement of enteroviruses by magnesium chloride, cysteine, and pancreatin. J. Bacteriol. 91:1932-1935. 1966.-Plaque formation of 21 echoviruses (types 1-6, 9, 13, 15-19, 23-26, 29-32) and 8 coxsackieviruses (B1-6, A7, and A9) was enhanced by increased concentrations of MgCl(2), l-cysteine, and pancreatin in agar overlay medium. In most cases, cationic and anionic polymers (diethylaminoethyl dextran, dextran sulfate, or protamine sulfate) were ineffective. All strains of poliovirus and group B coxsackieviruses were enhanced under agar by MgCl(2). Five of the eight coxsackieviruses tested were also enhanced by cysteine or pancreatin. Certain enteroviruses, which have been difficult to assay by plaque method, can now be quantified effectively by incorporation of additives such as MgCl(2), cysteine, or pancreatin into the overlay medium.


Identification of enteroviruses by hemagglutination-inhibition. Kern, Jerome (Pacific Research Section, Honolulu, Hawaii), and Leon Rosen. Identification of enteroviruses by hemagglutination-inhibition. J. Bacteriol. 91:1936-1942. 1966.-Approximately 40% of a group of 906 enterovirus isolates cytopathic for monkey cell cultures were found to possess hemagglutinins for human erythrocytes when tested at temperatures of 4 and 37 C and at pH 5.8 and 7.3. The hemagglutinating isolates could be classified by relatively simple techniques into 18 serotypes. Four of these serotypes, echovirus type 24 and coxsackievirus B types 1, 5, and 6, had not previously been known to include hemagglutinating strains. One serotype, Toluca-3, represented a previously unrecognized enterovirus, and two other serotypes may also represent previously unrecognized enteroviruses.


Effect of poliovirus on deoxyribonucleic acid synthesis in HeLa cells. Ackermann, W. W. (University of Michigan, Ann Arbor), D. C. Cox, H. Kurtz, C. D. Powers, and S. J. Davies. Effect of poliovirus on deoxyribonucleic acid synthesis in HeLa cells. J. Bacteriol. 91:1943-1952. 1966.-Both poliovirus and arginine stimulated deoxyribonucleic acid (DNA) synthesis in cultures of HeLa cells which were preconditioned by incubation in a medium deficient in arginine. However, the number of cells producing DNA was unaffected. DNA synthesis in such preconditioned cells was 10 to 20% of the maximal value obtained with a full complement of amino acids. Inhibition of DNA synthesis was produced in these cultures either by increasing the multiplicity of exposure above 40 plaque-forming units of virus per cell or by increasing the concentration of the deficient amino acid at the time of virus addition. Inhibition of DNA synthesis resulted from a reduction in the fraction of cells producing DNA. The concentration of arginine required for viral inhibition of DNA synthesis is greater than that for viral multiplication.


Yaba tumor poxvirus synthesis in vitro. II. Adsorption, inactivation, and assay studies. Yohn, David S. (Roswell Park Memorial Institute, Buffalo, N.Y.), Fanny R. Marmol, Victoria A. Haendiges, and James T. Grace, Jr. Yaba tumor poxvirus synthesis in vitro. II. Adsorption, inactivation, and assay studies. J. Bacteriol. 91:1953-1958. 1966.-Means to increase the efficiency of the Yaba tumor poxvirus assay in BSC-1 cell cultures were sought. A method was devised wherein 0.4 ml of each virus dilution was layered onto BSC-1 cells in 80-mm Leighton tubes and permitted to adsorb at 25 C for 18 hr prior to incubation at 35 C. The diluent found most reliable was medium 199 containing 50% bovine amniotic fluid adjusted to 2.0 mm calcium and 1.0 mm magnesium at pH 7.0. These modifications yielded highly reproducible titrations with a greater than twofold increase in assay sensitivity. Irreversible adsorption of Yaba virus by BSC-1 cells proceeds comparatively slowly at 25 to 33 C. Although the process is more rapid at 35 or 37 C, the increased thermal lability of the virus at these temperatures results in lower titers than with virus adsorbed at 25 C for longer periods of time. Yaba poxvirus appears to be 5 to 10 times more sensitive to thermal inactivation than vaccinia poxvirus.


Persistent cyclic herpes simplex virus infection in vitro. II. Localization of virus, degree of cell destruction, and mechanisms of virus transmission. Hampar, Berge (National Institute of Dental Research, Bethesda, Md.). Persistent cyclic herpes simplex virus infection in vitro. II. Localization of virus, degree of cell destruction, and mechanisms of virus transmission. J. Bacteriol. 91:1959-1964. 1966. The localization of virus, degree of cell destruction, and mechanisms of virus transmission in persistent herpes simplex virus-infected cultures were studied. The major fraction of infectious virus was associated with the medium and a minor fraction was associated with the attached cells. Virus in the medium was further separable into a sedimentable (cellular) fraction and a nonsedimentable (extracellular) fraction. The sedimentable fraction was comprised of cellular debris, most of which appeared to contain viral antigen, and intact cells of which less than 10% contained infectious virus. Cell destruction during the cycle involved more than 99.9% of the maximal number of cells present. Infection could be transmitted by extracellular virus, cell-to-cell transfer, and reattachment of infectious cellular material. The results indicated that transmission by reattachment was probably mediated through the cellular debris rather than the intact cells.


Comparative study of seven picornaviruses of man. Jamison, Richard M. (Baylor University College of Medicine, Houston, Tex.), and Heather D. Mayor. Comparative study of seven picornaviruses of man. J. Bacteriol. 91:1971-1976. 1966.-The buoyant densities in CsCl and potassium tartrate of seven acid-resistant picornaviruses of man were determined. The viruses studied were found to be a relatively homogeneous group. Buoyant densities ranged from 1.32 to 1.35 g/cc in CsCl and 1.21 to 1.23 g/cc in potassium tartrate. The buoyant density of each virus did not vary by more than 0.01 g/cc. The host cell of origin and passage level did not influence the buoyant density of the virions. However, measurements of the diameters of purified virions by a standard method have shown that there are considerable size variations among these picornaviruses. The diameters ranged from 18 to 25 mmu. Echovirus 19 and poliovirus 1 were significantly larger (mean diameter, 23.5 mmu) than echoviruses 4, 6, 16, and 23, and coxsackievirus B2 (mean diameter, 21.0 mmu).


Studies on Japanese B encephalitis virus vaccines from tissue culture. VI. Development of a hamster kidney tissue culture inactivated vaccine for man. (1). Obtaining maximum titers of virus using an attenuated strain of OCT-541.


Yaba tumor poxvirus synthesis in vitro. I. Cytopathological, histochemical, and immunofluorescent studies. Yohn, David S. (Roswell Park Memorial Institute, Buffalo, N.Y.), Victoria A. Haendiges, and James T. Grace, Jr. Yaba tumor poxvirus synthesis in vitro. I. Cytopathological, histochemical, and immunofluorescent studies. J. Bacteriol. 91:1977-1985. 1966.-Yaba tumor poxvirus synthesis in BSC-1 cell culture was followed sequentially with light microscopy, immunofluorescent microscopy, and various histochemical stains. The first evidence of infection was detected at 24 hr when nucleoli became hypertrophic, reflecting enhanced ribonucleic acid (RNA) synthesis. At 36 hr, deoxyribonucleic acid synthesis was detected in the cytoplasm. This was immediately followed by or associated with antigen synthesis at paranuclear sites and enhanced RNA synthesis in the cytoplasm. Cytoplasmic inclusions were readily apparent at 4 days in initially infected cells. Contiguous spread of virus was judged to have occurred around the third day of infection. The time required to complete the synthetic cycle from time of infection to production of infectious progeny was estimated to be of the order of 60 hr. This cycle is 6 to 10 times longer than for vaccinia virus. By light microscopy, cytopathic effects (CPE) were detectable in 5 days in heavily infected cultures. With 100 units or less of infectious virus, CPE was not readily detected until the 10th to 14th day. At this time, focal areas of infection classified either as microtumors or microplaques were present. Secondary foci appeared during the 4th week of incubation.


Immunogenicity of inactivated parainfluenza type 1, type 2, and type 3 vaccines in infants.


Interference in mouse leukemia: results and prospects.


Subcellular particles associated with human leukemia as seen with the electron microscope.


Granulosa- and theca-cell tumours and genital cancer.


Cooperative interaction between the GTP binding sites of glutamate dehydrogenase.


Antibody response of fish to viral antigens.


A nuclear magnetic resonance study of substrate binding by yeast alcohol dehydrogenase.


Isolation of 4-O-beta-N-acetylmuramyl-N-acetylglucosamine and 4-O-beta-N, 6-O-diacetylmuramyl-N-acetylglucosamine and the structure of the cell wall polysaccharide of Staphylococcus aureus.


Intracellular distribution of glucose-6-phosphatase  and glycogen content in the developing chick liver.


The role of epithelium and mesenchyme in the production of a collagenolytic enzyme and a hyaluronidase in the anuran tadpole.


The metabolism of homogenates of the mouse epididymis.


The effect of efferentiectomy and orchidectomy on the metabolism of the epididymis of the mouse.


Transformation of hamster embryo cells in vitro by polyoma virus: morphological, karyological, immunological and transplantation characteristics.


Peripheral neuromyopathy following chloroquine therapy.


Gastric antisecretory and other pharmacologic studies on 2,2'-bipyridine.


Effects of pituitary hormones and prefeeding N-hydroxy-N-2-fluorenylacetamide on the metabolism of this carcinogen and on physiologic parameters.


Immunology of the mouse mammary tumor virus (MTV) : identification in vitro of mouse antibodies against MTV.


Non-respiratory oscillations in systemic arterial pressure of dogs.


Hodgkin's granuloma complicated by generalized cytomegalic inclusion disease and gastrointestinal moniliasis. A case is described in which Hodgkin's disease was complicated by inclusion disease and gastrointestinal moniliasis. This is one of several cases of malignant disease complicated by uncommon infections.


Pedunculated islet-cell tumour of the duodenum. An unusual islet-cell tumour found at necropsy in a patient who had died from a myocardial infarction is described. Of particular interest were the pedunculated nature and large size of the tumour. The clinical features of the case are considered. Four islet-cell tumours in the duodenum have previously been reported and it seems probable that such tumours arise in heterotopic pancreas.


Nitrofurantoin and peripheral neuropathy with megaloblastic anaemia.


Assessment of hypothalamic pituitary function in endocrine disease. The insulin test carried out with adequate safeguards under standardized conditions yields valuable information regarding hypothalamic and pituitary function when plasma levels of sugar, cortisol, and growth hormone are determined. The use of a test based on the plasma cortisol response to the infusion of lysine-vasopressin, a polypeptide with a corticotrophin-releasing action, is also of value as a test of pituitary function. Used in conjunction with the insulin test it enables pituitary disorders to be differentiated from those involving the hypothalamus.


Antibiotic effect of tylosin on a mycoplasma contaminant in a tissue culture leukemia cell line.


Dissociation of adult mouse liver by sodium tetraphenylboron, a potassium complexing agent.


Effects of a hepatocarcinogenic diet on adrenal glands and liver of rats.


Collagenase activity of gingival tissue from patients with periodontal disease.


Relationship between the lactic dehydrogenase-elevating virus and transplantable murine tumors.


Virus-induced transplantation immunity to human adenovirus type 12 tumors of the hamster and mouse.


Hydrocephalus in mice infected with polyoma virus.


Attempt at immunization by oral feeding of live rhinoviruses in enteric-coated capsules.


Skeletal response to exogenous ascorbic acid by vitamins D3 deficient chicks.


The ultrastructure of acidophilic "Councilman-like" bodies in the liver.


Pharmacological and biochemical studies on ethambutol in laboratory animals.


Clinical and pathological diagnosis.

Clinical and pathological diagnosis.


Effects of postnatal protein deficiency on weight gain, serum proteins, enzymes, cholesterol, and liver ultrastructure in a subhuman primate (Macaca mulatta).


Practical management of the nephrotic syndrome. Alternate day steroids and immunosuppressive therapy.


Temperature dependence of sodium ion activation of the cerebral microsomal adenosine triphosphatase.


Complications and difficulties in electrocoagulation of varices of the lower extremities.


Lymphangiographic studies in obstructive lymphedema of the upper extremity.


The syntheses of peptides related to the N-terminal structure of corticotropin. VII. The synthesis of peptide derivatives corresponding to positions 11 to 18 in the corticotropin molecule.


Changes in electron spin resonance signals of rat liver during chemical carcinogenesis.


Isolation of skeletal muscle nuclei.

Isolation of skeletal muscle nuclei. A method employing aqueous media for isolation of nuclei from rat skeletal muscle is described. The technique involves (a) mincing and then homogenizing in a 0.32 M sucrose-salt solution with a Potter-Elvehjem type homogenizer using a Delrin (an acetal resin) pestle and a carefully controlled, relatively large pestle-to-glass clearance, (b) filtering through fiberglass and stainless steel screens of predetermined mesh size to remove myofibrils and connective tissue, and (c) centrifuging in a 2.15 M sucrose-salt solution containing 0.7 mM ATP. Electron and phase-contrast microscopic observations show that the nuclei are intact, unencumbered by cytoplasmic tags, and possess well preserved distinct nucleoli, nucleoplasm, and nuclear membranes. Cytoplasmic contamination is minimal and mainly mitochondrial. Chemical assays of the nuclear fraction show that the DNA/protein and RNA/DNA ratios are comparable to those obtained in other tissues. These ratios, as well as the low specific activity obtained for cytochrome c oxidase and the virtual absence of myofibrillar ATPase, indicate a high degree of purity with minimal mitochondrial and myofibrillar contamination. The steps comprising the technique and the reasons for their selection are discussed.


Necrosis of the pancreas, parotid and lachrymal glands associated with encephalomyocarditis virus infection.


A theory on the mechanism of carcinogenesis by small deoxyribonucleic acid tumour viruses.


Interaction of 4-dimethylaminoazobenzene and dietary fat in the regulation of hepatic microsomal ascorbic acid synthesis.


Inhibition of aortic calcification by means of pyrophosphate and polyphosphates.


Altered cardiac retention of exogenous noradrenaline produced by stress in young rabbits.


Effect of gossypol on some oxidative respiratory enzymes. Gossypol was examined in relation to its effect on certain enzymes and enzyme complexes associated with the tricarboxylic acid cycle and the electron transport system. Succinic dehydrogenase and cytochrome oxidase activity from sweet potato was completely inhibited by gossypol at 7.5 x 10(-3)m and 2.0 x 10(-3)m, respectively. Succinoxidase activity of the same preparations was fully inhibited at a lower concentration, 2.5 x 10(-4)m. This concentration did not affect either succinic dehydrogenase or cytochrome oxidase, the primary and terminal enzymes of the succinoxidase complex. The nature of the intermediate step or steps inhibited at this concentration is not yet known. Gossypol was further shown to inhibit phosphorylation at concentrations having no appreciable effect on oxidation. Inhibition in general was not reduced by increased substrate concentrations in the enzyme systems examined, with the exception of cytochrome c for cytochrome oxidase. Bovine serum albumin was partially effective in reducing gossypol inhibition, provided that it was present before enzyme exposure to gossypol.


Radiocalcium absorption at different times of day.


Insulin-induced hypoglycemia as a test of pituitary-adrenal function in thyrotoxicosis.


Postoperative studies of adrenal function in primary aldosteronism.


1-Adamantanamine hydrochloride: inhibition of Rous and Esh Sarcoma viruses in cell culture. I-Adamantanamine hydrochloride, added to chick embryo cells, inhibited focus production upon subsequent inoculation of the cells with Rous and Esh sarcoma viruses. Addition of the chemical to suspensions of Rous and Esh viruses before they were added to chick cell cultures did not inactivate the viruses; and the addition to chick cells did not prevent plaque formation following their inoculation with Newcastle disease virus. This indicates that cells treated with 1-adamantanamine hydrochloride are capable of supporting viral replication, and these observations suggest that the drug inhibits growth of two oncogenic viruses by prevention of virus penetration, but not by a virucidal effect or cell intoxication.


Studies on the pathogenicity of various strains of Entamoeba histolytica after prolonged cultivation, with observations on strain differences in the rats employed.


Polysomes and protein synthesis in cells infected with a DNA virus. In HEp-2 cells infected with herpes simplex virus the rate of protein synthesis at first decline, is stimulated between 4 and 8 hours after infection, and progressively and irreversibly declines from 9 to 16 hours later. The increase and decrease in rates coincide with corresponding changes in the amounts of cytoplasmic polysomes and amounts of labeled amino acids in nascent peptides bound to polysomes. The data indicate that (i) early and late inhibition and intervening stimulation of protein synthesis are due to the corresponding breakdown and formation of polysomes, and (ii) the bulk of viral proteins is probably made on cytoplasmic polysomes.


Studies of arthropod-borne virus infections in Chiroptera. 3. Influence of environmental temperature on experimental infection with Japanese B and St. Louis encephalitis viruses.


Studies of arthropod-borne virus infections in Chiroptera. IV. The immune response of the big brown bat (Eptesicus f. fuscus) maintained at various environmental temperatures to experimental Japanese B encephalitis virus infection.


Cytomegalic inclusion disease. An electron microscopic histochemical study of the virus at necropsy.


Experimental myocardial infarction. A histochemical study.


Experimental endocarditis. Endocrine factors in valve lesions on AV shunt rats.


Production of hypertension and vascular disease by angiotensin.


Pentobarbital sleeping time and RES stimulation.


Studies on the hand in ulnar nerve paralysis. A clinical-experimental investigation in normal and anomalous innervation.


Conformational changes and the regulation of glutamate-dehydrogenase activity. 1. The effect of NADH and the non-competitive inhibitor GTP on the optical-rotatory-dispersion properties of glutamate dehydrogenase has been studied. 2. Analysis of the data in terms of the a(0) and b(0) parameters of the Moffitt-Yang equation indicates that a conformational change is induced either by NADH or by GTP in the presence of small amounts of NADH. 3. Sedimentation measurements under comparable conditions showed that the enzyme reversibly dissociates into sub-units but that this dissociation is only secondary to the conformational changes. 4. Fluorescence measurements showed that the binding constant of NADH and the number of binding sites on the enzyme increased in the presence of GTP. 5. This is confirmed by studies of fluorescence polarization, which in addition showed that the movement of NADH on the enzyme surface is more restricted in the presence of GTP. 6. The relation of these results to possible regulatory mechanisms is discussed.


Studies on the biosynthesis of the corrin ring of vitamin B 12.


A rat-tissue aminotransferase acting on L-tyrosine O-sulphate. 1. Rat tissues have been shown to possess an aminotransferase that is active towards l-tyrosine O-sulphate and dependent on 2-oxoglutarate and pyridoxal phosphate. 2. Kidney, liver and pancreas have the greatest activity and the enzyme is localized mainly in the mitochondrial fraction in the liver and kidney cell. 3. The enzyme was shown to be distinct from l-tyrosine-2-oxoglutarate aminotransferase but its true identity was not established. 4. A procedure for the assay of the enzyme in crude tissue preparations was developed.


The differentiation and assay of vitamins D2 and D3 by gas-liquid chromatography. 1. A method is described for the differentiation and determination of as little as 0.2mug. of vitamins D(2) and D(3) by gas-liquid chromatography. 2. The vitamins are converted by treatment with antimony trichloride into isovitamins D(2) and D(3), which show single, separate peaks on gas-liquid chromatography, unlike the unmodified vitamins, which give twin peaks due to the formation of pyro and isopyro derivatives. 3. Since isovitamins D(2) and D(3) remain together in all steps of the procedure except during gas-liquid chromatography, one may be used as an internal standard for the other. 4. The use of an internal standard reduces the importance of loss during sample preparation and increases precision. 5. The application of the method to biological materials is demonstrated.


Incorporation of injected [32P] phosphate into the phosphoinositides of subcellular fractions from young rat brain. 1. Six subcellular fractions were prepared from the brain of 3-week-old rats after the injection of [(32)P]orthophosphate into the subarachnoidal space. The fractions were characterized by chemical and enzymic determinations and by electron microscopy. 2. The highest concentration of phosphoinositides was found in the microsomal fraction. 3. After an exchange period of 4hr. in vivo, the specific radioactivity of phosphatidylinositol was high in the nuclear, mitochondrial and supernatant fractions, and that of diphosphoinositide was high in the nuclear, microsomal and supernatant fractions. Triphosphoinositide specific activity was highest in the myelin fraction. 4. Specific activities (counts/100sec./mug. of P) were in the following order for all fractions except myelin: diphosphoinositide>phosphatidylinositol>triphosphoinositide. For myelin the order was: diphosphoinositide>triphosphoinositide>phosphatidylinositol. 5. Phosphatidylinositol extracted from a tissue fraction by acidified chloroform-methanol had roughly twice the specific activity of that extracted by neutral solvents. The same applied to diphosphoinositide.


The incorporation of thymidine into deoxyribonucleic acid in mouse fibroblasts infected with polyoma virus. 1. Mouse-fibroblast cultures in the stationary phase of growth show an increased rate of [(3)H]thymidine incorporation into DNA from 12 to 44hr. after infection with polyoma virus. 2. Intracellular virus progeny is first detected at about 24hr. after infection. 3. Calculations based on the [(3)H]thymidine-incorporation data and direct measurements of the DNA content of the cell cultures indicate that the amount of the excess of DNA synthesized by the infected cell cultures corresponds to about 10% of their total DNA. 4. The mitotic index of the cell cultures at 40hr. after infection was significantly higher than that of non-infected control cells. 5. Possible interpretations of the stimulation of DNA synthesis observed in polyoma-infected cell cultures are discussed.


Giant malignant tumor of the mouth; unusually long follow--up.


The inhibition of enzymes by beryllium.

The inhibition of enzymes by beryllium. 1. The action of beryllium on the following enzymes has been examined: alkaline phosphatase (Escherichia coli and kidney), acid phosphatase, phosphoprotein phosphatase, apyrase (potato), adenosine triphosphatase (liver nuclei, liver mitochondria, brain microsomes), glucose 6-phosphatase, polysaccharide phosphorylases a and b, phosphoglucomutase, hexokinase, phosphoglyceromutase, ribonuclease, A-esterase (rabbit serum), cholinesterase (horse serum), chymotrypsin. Alkaline phosphatase and phosphoglucomutase are inhibited by 1mum-beryllium sulphate whereas the other enzymes are largely unaffected by 1mm-beryllium sulphate. 2. Possible mechanisms for the inhibition of phosphoglucomutase and alkaline phosphatase are discussed.


The contribution of the carpal bones to the assessment of skeletal age.


Effects of intravitreous injection of drugs on the cholinesterase of the retina.


Studies on a skin calcifying system.

Studies on a skin calcifying system.


The suitability of silvered leaf monkey (Presbytis cristatus) kidney cell cultures for testing poliovirus vaccines.


The effect of some sulfur-containing pyridine derivatives on the carbohydrate metabolism of Ehrlich ascites tumor.


Studies on 2-(alpha-hydroxybenzyl)benzimidazole (HBB) analogs. I. Synthesis of 8-(alpha-hydroxybenzyl)purines, the diaza analogs of HBB.


Studies on the transfer of subviral infectivity from SV40-induced hamster tumor cells to indicator cells.


Radiation studies of avian tumor viruses and of Newcastle disease virus.


Deoxyribonucleic acid synthesis in cells replicating polyoma virus.


Properties of cells derived from adenovirus-induced hamster tumors by long-term in vitro cultivation. II. Nature of the restricted response to type 2 adenovirus.


Lymphatico-venous communications and lymph reflux after thoracic duct obstruction. An experimental study in the dog.


Cardiac lymphangiography. A preliminary report on a roentgen study of the cardiac lymphatics in the dog.


The inhibition of poliovirus growth by actinomycin D and the prevention of the inhibition by pretreatment of the cells with serum or insulin.


Changes in metabolic and enzymatic activities of monkey kidney cells after infection with adenovirus 2.


Electron microscopic localization of virus-associated antigens in human amnion cells (AV-3) infected with human adenovirus, type 12.


Antagonism of the guanidine interference with poliovirus replication by simple methylated and ethylated compounds.


High frequency of SV40 transformation of mouse cell line 3T3.


The effect of SV40 infection on DNA synthesis in cercopithecus kidney cells.


In vitro transformation of rat embryo cells by a murine sarcoma virus.


Counteraction between interferon and enhancer.


Studies on the alteration of species-specific resistance by ethionine-induced inhibition of protein metabolism. II. Infection experiments in rats with canine hepatitis (Rubarth-disease) virus.


Plant tissue phosphodiesterase activities.


A novel branched-chain sphingolipid base from Crithidia fasciculata.


Purification and properties of nucleoside tetraphosphate hydrolase from rabbit muscle.


Purification and properties of 3',5'-cyclic nucleotide phosphodiesterase from dog heart.


Biosynthesis of uridine diphosphate D-xylose. II. Uridine diphosphate D-glucuronate carboxy-lyase of Cryptococcus laurentii.


Biosynthesis of phosphatidyl myoinositol phosphates in rabbit brain.


Electron-transport systems of yeast. II. Purification and properties of a soluble reduced diphosphopyridine nucleotide dehydrogenase.


Improvement in methodology for measurement of plasma renin activity.


Activity of glycolytic and hexose monophosphate pathways in Yoshida ascites hepatomas.


Studies on tissue culture of ascites tumors. I. Primary culture of rat ascites tumors.


Studies on tissue culture of ascites tumors II. In vitro growth of mouse ascites hepatoma MH 134.


Studies of induced malignant tumors of placental and uterine origin in the rat. I. Survival of placental tissue following fetectomy.


Prophylactic chemotherapy of hydatidiform mole.


Reconstruction of the hand in high median nerve injuries.


A test of adrenal cortical function in dairy cows by ACTH administration.


Stiulation of growth of Bordetella pertussis by acetate.


Silica.

Silica.


Treatment of abortions infected with Clostridium welchii.


Idiopathic non-inflammatory neuromyopathy.


Tropical sprue: viral cultures of rectal swabs.


Effect of adrenal stimulation on histamine in the rat stomach.


Chemotherapy of malignant lesions unique in children.


Hypoglycemia.

Hypoglycemia.


Vasoactive polypeptides of surgical significance.


Effect of Naja naja atra venom on cytochrome C oxidase, L-ascorbic acid oxidase, peroxidase, and catalase.


Growth requirements and biologic characteristics of leukemia L1210 cells in culture.


Persistent cyclic herpes simplex virus infection in vitro. 3. Asynchrony in the progression of infection and cell regrowth. Hampar, Berge (National Institute of Dental Research, Bethesda, Md.). Persistent cyclic herpes simplex virus infection in vitro. III. Asynchrony in the progression of infection and cell regrowth. J. Bacteriol. 91:1965-1970. 1966.-The progression of virus-induced cytopathic effects (CPE) and virus synthesis was studied in localized areas of Chinese hamster cell cultures persistently infected with herpes simplex virus (HSV). CPE was initially evidenced by the presence of small multinucleated giant cells, followed by expanding plaquelike lesions with an occasional uninfected cell remaining within the infected areas. Cell detachment rapidly followed the appearance of viral antigen in infected cells. The surviving cells which proliferated to re-establish the cell sheet arose from two sources. The first was from viable cells which remained attached after expansion of localized areas of CPE, and the second was from reattachment of viable cells in the medium. CPE in localized areas was initiated at various times during the cycle irrespective of the virus titer in the medium. Cell regrowth in some areas and CPE in other areas occurred simultaneously throughout the cycle in an asynchronous fashion. Consequently, during periods of rising virus titers, most areas showed CPE while few areas displayed cell regrowth. As the virus titers declined, more areas showed cell regrowth and fewer areas displayed new cycles of CPE. CPE in localized areas was not initiated until cell regrowth had occurred. It is proposed that the proliferating cells were temporarily resistant to HSV infection, and that this resistance was ultimately lost in their progeny cells.


Yaba tumor poxvirus synthesis in vitro. 3. Growth kinetics. Yohn, David S. (Roswell Park Memorial Institute, Buffalo, N.Y.), Victoria A. Haendiges, and Etienne de Harven. Yaba tumor poxvirus synthesis in vitro. III. Growth kinetics. J. Bacteriol. 91:1986-1991. 1966.-The infectious synthetic cycle of Yaba poxvirus by BSC-1 cells at 35 C is described and discussed together with related observations obtained by immunofluorescence and electron microscopy. After adsorption of virus at 25 C, at least 6 hr of incubation at 35 C was required before viral eclipse was demonstrable. Infectious progeny appeared after an additional 48 hr of incubation at 35 C. Newly synthesized virus particles were seen at 68 hr but not at 44 hr by electron microscopy. Maximal maturation occurred between 100 and 120 hr. Maximal yields were obtained from the 5th through the 8th day. The differences in kinetics between Yaba and vaccinia poxviruses, as described for the latter in the literature, are discussed. Possible factors accounting for these differences are enumerated.


Studies of the mechanism through which sodium depletion increases aldosterone biosynthesis in man.


Animal--man cancer probes continue.

Animal--man cancer probes continue.


Infusion of floxuridine in the treatment of solid tumors.


Necrotizing colitis due to Clostridium welchii.


Some recent work at the Common Cold Research Unit.


A long-term study of acute respiratory diseases in a natural community.


Effect of renin, angiotensin II and aldosterone on erythropoiesis.


The effects of terminating and detraining on enzyme activities of heart and skeletal muscle of trained rats.


The isolation of retinal outer segment fragments. Bovine retinal outer segment fragments were isolated by density gradient centrifugation in a high centrifugal field. Assays of the final preparation for enzymes of the mitochondrial respiratory chain indicated mitochondrial contamination not in excess of 1 per cent. Glucose-6-phosphatase and TPNH-cytochrome c reductase activities, presumably diagnostic for microsomes, were also absent. Electron micrographs did not disclose the presence of significant numbers of particles other than fragments of the outer segment discs. The red fragments were characterized by an ascorbate-oxidizing system and a high lipid content.


Ribonucleic acid and protein synthesis in mitotic HeLa cells. HeLa cells arrested in mitosis were obtained in large numbers, with only very slight interphase cell contamination, by employing the agitation method of Terasima and Tolmach, and Robbins and Marcus. Protein synthesis and RNA synthesis were almost completely suppressed in mitotic cells. Active polyribosomes were nearly absent in mitotic cells as compared with interphase cells treated in the same way. Cell-free protein synthesis and RNA polymerase activity were also greatly depressed in extracts of metaphase cells. The deoxyribonucleoprotein (DNP) of condensed chromosomes from mitotic cells was less efficient as a template for Escherichia coli RNA polymerase than was DNP from interphase cells, although isolated DNA from both sources was equally active as a primer. Despite very poor endogenous amino acid incorporation by extracts of metaphase cells, polyuridylate stimulated phenylalanine incorporation by a larger factor in mitotic cell extracts than it did in interphase cell extracts. These results suggest that RNA synthesis is suppressed in mitotic cells because the condensed chromosomes cannot act as a template, and that protein synthesis is depressed at least in part because messenger RNA becomes unavailable to ribosomes. This conclusion was supported by the demonstration that cells arrested in metaphase supported multiplication of normal yields of poliovirus, thereby showing that the mitotic cell is capable of considerable synthesis of RNA and protein.


Subcutaneous sarcomata in hamsters induced by Rous sarcoma virus (Bryan).


Derivatives of 2-aminoethyl-guanidine and of a eugenol as virus inhibitors.


Rise of cytomegalovirus antibodies in an infectious-mononucleosis-like syndrome after transfusion.


The structure of glycopeptide obtained from Taka-amylase A.


The acid-soluble nucleotides of milk. IV. The chemical structure of UDP-X3.


A possible role for cytochrome b-555 in the mung bean mitochondrial electron transport system.


Diagnostic and physiologic implications of the angiotensin infusion test.


Angiotensin infusions.

Angiotensin infusions.


Estimation of free methylpentoses in the presence of glycosidically bound sugar.


Observations on epiphyseal fusion of the canine appendicular skeleton.


Usefulness of the brain scan in therapeutic radiology.


Effect of chemical agents on foot-and-mouth disease virus production in cell cultures.


Determination of 17-hydroxycorticosteroids in canine urine.


Oxidative activity of crab gill mitochondria as a function of osmotic concentration.


Deamidation of adenosine phosphoramidate by hog eye ciliary process.


Amino acid activating enzymes in Sarcophaga bullata.


Active cation transport and ATP hydrolysis in Acanthamoeba sp.


A mixed layer for separation of amino acids by thin-layer chromatography.


A sensitive reagent for detecting 2-deoxysugars and 3-deoxypolyols.


Thin-layer chromatography on silica gel: quantitative analysis by direct U.V. spectrophotometry.


Transesterification of lecithin on silicic acid columns.


Pancreatic cysts occurring secondary to ductal carcinoma of the pancreas.


The angiotensin infusion test: preliminary experience.


Granular cell myoblastoma of the pituitary body simulating a chromophobe adenoma.


Wilms' tumor--metastatic to the lung. Report of a survivor with radiation therapy only.


Viraemia following polio vaccination with Sabin type 1 strain LSc2ab.


Aspects of the synthesis of poliovirus RNA and the formation of virus particles.


Genetic basis of cellular susceptibility to the Schmidt-Ruppin and Harris strains of Rous sarcoma virus.


Viral causes of bovine abortion in Ohio.


Clostridium perfringens myometritis complicating cesarean section. Report of 2 cases.


Extracranial metastases from central nervous system tumors in children and adolescents.


An analysis of circadian rhythms in human adrenocortical secretory activity.


Studies on the mechanism of nidation. XIX. Histochemical changes in the ovaries of pregnant rats following ergocornine.


A simple method for measuring ribonucleic acid content of preparations of deoxyribonucleic acid.


Action of angiotensin on sympathetic nerve endings in isolated blood vessels.


Inhibition of adrenocortical responsiveness to ACTH by actinomycin D in vivo.


Antiviral activity of p-nitrobenzylphosphonic acid.


Haemolytic activity of suspensions of different silica modifications and inert dusts.


Inhibition of encephalomyocarditis virus replication by simple phosphonic and carboxylic acids.


Distribution of the nucleases of the microsomal fraction of rat liver between ribosomes and endoplasmic membranes.


The localization of ulnar nerve compression in the hand and arm: an improved method of electroneuromyography.


Energy-linked reactions in mitochondria. Studies on the reduction of NAD+ by succinate.


Thermodynamic properties of an allosteric transition.


Anal and perianal malignant neoplasms: pathology and treatment.


The properties of a glycyl-soluble-RNA synthetase from chick embryo.


Influence of substrate-induced enzymic inactivation on the sulfhydryl content of glyceraldehyde-3-phosphate dehydrogenase.


Ribonucleases of the rat. 3. Localization by immunofluorescence.


Comparison of guanosine triphosphate split and polypeptide synthesis with a purified E. coli system.


Vitamin A and the sulfate-activating enzymes.


Potentiation of oncogenicity of adenovirus type 12 grown in African green monkey kidney cell cultures preinfected with SV40 virus. Persistence of both T antigens in the tumors and evidence for possible hybridization.


Inhibition of hyaluronic acid degradation by dimethyl sulphoxide.


Effect of ethylenediamine tetraacetate on the resistance of Pseudomonas aeruginosa to antibacterial agents.


Electron spin resonances in Formicidae.

Electron spin resonances in Formicidae.


Relation of cell size to the effect of x-rays on poliovirus production by monkey-kidney cells.


Vesicular exanthem of mixed bacterial-viral etiology.


Nutritive value of Nigerian water melon.


Structure of the pigments of precooked irradiated meats.


Effect of aerobic pre-incubation of glycolysis in resting and proliferating liver cells.


Transport of S-antigen of Sendai virus as revealed by means of antibody labelled with an organic mercury compound.


Antibiotic carry-over in cell cultures of herpes simplex virus.


The bacterial degradation of pantothenic acid. 3. Enzymatic formation of aldopantoic acid.


The bacterial degradation of pantothenic acid. IV. Enzymatic conversion of aldopantoate to alpha-ketoisovalerate.


The glycolipids of dog intestine.

The glycolipids of dog intestine.


Healing following simple gingivectomy. A tritiated thymidine radioautographic study. I. Epithelialization.


Primary oxidation-reduction changes during photosynthesis in Rhodospirillum rubrum.


New acetylenic fatty acids from Acanthosyris spinescens seed oil.


A simplified method for the extraction and bioassay of renin.


Studies on the transplantation of ascites hepatoma (AH 7974) into rat's uterine cervix.


Electron spin resonance spectra of gamma-irradiated polycrystalline glycollic acid.


Demonstration of an avian leucosis group antigen by immunodiffusion.


Morphology of components of Sendai virus.


Determination of the type of nucleic acid in rubella virus using 5-bromo-2'-deoxyuridine.


Cell growth and the initiation of transformation by SV40.


Hormone control of connective tissue.

Hormone control of connective tissue.


Involvement of CO2 in the Hill reaction.


Nucleic acid homology studies of adenovirus type 7-SV40 interactions.


Energy transfer in dinucleotides.

Energy transfer in dinucleotides.


Immunofluorescent studies of group-specific antigen of the avian sarcoma-leukosis viruses.


Viral infections of monkeys in their natural habitat in southern India. II. Serological evidence of viral infection.


Isolation of Japanese encephalitis virus from the blood of a child in Singapore.


Immunological studies with group B arthropod-borne viruses. V. Evaluation of cross-immunity against type 1 dengue fever in human subjects convalescent from subclinical natural Japanese encephalitis virus infection and vaccinated with 17D strain yellow fever vaccine.


Cultivation of Entamoeba histolytica and Entamoeba histolytica-like strains at reduced temperature and behavior of the amebae in diluted media.


Effect of amytal, pyridine nucleotides, and oxaloacetate on the respiratory inhibition-activation cycles in mitochondria.


The intracellular nucleotides of Astasia longa.


Ribonucleoside triphosphatase in rabbit reticulocytes.


Purification and properties of an inhibitor for nicotinamide-adenine dinucleotidase from Mycobacterium tuberculosis H-37-Rv.


Increase of mitochondrial content in sweet potato root tissue after wounding.


Membrane isolation and cytoskeletal breakdown. II. Enzyme studies revealing cytoskeletal stabilization by FAD.


Erythropoietic differentiation of stable cell lines derived from hematopoietic organs of mice with virus-induced leukemia.


Selective response of the Japanese quail to various strains of Rous sarcoma virus. Shipman, Charles Jr. (Indiana University School of Medicine, Indianapolis), and Alvin S. Levine. Selective response of the Japanese quail to various strains of Rous sarcoma virus. J. Bacteriol. 92:161-163. 1966.-The Bryan "high-titer," Carr-Zilber, Harris, Prague, Schmidt-Ruppin, and "29" strains of Rous sarcoma virus were used to initiate tumors in the Japanese quail (Coturnix coturnix japonica). Although all strains produced tumors upon primary inoculation, only the Bryan "high-titer" and Harris strains could be serially passaged with cell-free tumor extracts. Analysis by means of the end-point dilution technique and the interference test revealed that the tumor extract of the Bryan "high-titer" strain passaged 10 times in the quail contained a Rous-associated virus.


Selective inactivation of biological activity of myxoviruses by glutaraldehyde.


Effect of mycoplasma on interferon production and interferon assay in cell cultures. Armstrong, D. (The Children's Hospital of Philadelphia, Philadelphia, Pa.), and K. Paucker. Effect of mycoplasma on interferon production and interferon assay in cell cultures. J. Bacteriol. 92:97-101. 1966.-The influence of mycoplasma on the production and action of interferon was studied in cultures of both L and human embryonic kidney (HEK) cells. Mycoplasma hominis 1, the Negroni agent, and the F12 mycoplasma were used for infection of L cells, and M. hominis 1 and M. pneumoniae for inoculation of HEK cells. All strains were capable of multiplication in the culture systems employed. None produced detectable levels of interferon, and responsiveness of the cells to induction of interferon by virus remained unaltered. Infection with mycoplasma did not impair the sensitivity of the cells to the action of interferon, nor was the replication of vesicular stomatitis virus noticeably diminished.


The chemical synthesis and biological oxidation of 7-alpha-hydroxy[26-14C]cholesterol, 7-dehydro[26-14C]cholesterol and 26-hydroxy[26-14C]cholesterol. 1. 26-Hydroxycholesterol was obtained by reducing the methyl ester of (+/-)-3beta-hydroxycholest-5-en-26-oic acid, which was synthesized from 25-oxonorcholesterol. 2. Methods for preparing 7alpha-hydroxycholesterol and 7-dehydrocholesterol were modified to allow the micro-scale preparation of these [(14)C]sterols from [26-(14)C]-cholesterol. 3. 26-Hydroxycholesterol was oxidized more readily than 7alpha-hydroxycholesterol, 7-dehydrocholesterol or cholesterol by mitochondrial preparations from livers of mice, rats, guinea pigs, common toads (Bufo vulgaris) and Caiman crocodylus. 4. (+/-)-3beta-Hydroxy[26-(14)C]cholest-5-en-26-oic acid was oxidized very rapidly to (14)CO(2) by mouse and guinea-pig mitochondria without evident discrimination between the two optical isomers. 5. An enzyme system that oxidizes 26-hydroxycholesterol to 3beta-hydroxycholest-5-en-26-oic acid was identified in the soluble extract of rat-liver mitochondria. This enzyme could use NADP in place of NAD but was not identical with liver alcohol dehydrogenase (EC 1.1.1.1). 6. [26-(14)C]Cholesteryl 3beta-sulphate was not oxidized by fortified mouse-liver preparations that oxidized [26-(14)C]cholesterol to (14)CO(2).


The biohydrogenation of alpha-linolenic acid and oleic acid by rumen micro-organisms. 1. alpha-[U-(14)C]Linolenic acid was incubated with the rumen contents of sheep and the metabolic products were characterized by thin-layer chromatography, gas-liquid chromatography and absorption spectroscopy in the ultraviolet and infrared. 2. A tentative scheme for the biohydrogenation route to stearic acid is presented. The main pathway is through diconjugated cis-cis-cis-octadecatrienoic acid, non-conjugated trans-cis (cis-trans)-octadecadienoic acid and trans-octadecenoic acid, but other pathways are apparent. 3. Washed rumen micro-organisms possessed only a limited capacity to hydrogenate alpha-linolenic acid and oleic acid but the rate was greatly stimulated by a factor(s) present in the supernatant rumen liquor. 4. Pure cultures of Clostridium perfringens, Streptococcus faecalis, Escherichia coli and a coliform organism isolated from sheep faeces possessed negligible ability to hydrogenate unsaturated fatty acids compared with a mixed population of rumen micro-organisms. Butyrivibrio fibrisolvens slowly converted linoleic acid into octadecenoic acid.


Effects of prolonged incubation of isolated fat cells on their response to hormones stimulating lipolysis and glucose metabolism. 1. The metabolism of isolated fat cells from parametrial adipose tissue of starved normal rats was studied during 8hr. incubation. 2. There was a three- to eight-fold increase in conversion of glucose into carbon dioxide, fatty acids and glycerol during the fourth to eighth hours of incubation in 4% albumin buffer over that seen during the first 4hr. of incubation. 3. The addition of growth hormone and dexamethasone to fat cells at the start of the incubation period accelerated lipolysis during the first 4hr. of incubation but no further effect was seen during the fourth to eighth hours of incubation. Addition of growth hormone and dexamethasone to fat cells that had been incubated for 4hr. did not accelerate lipolysis during the next 4hr. whether fat cells were incubated with or without glucose. 4. Fat cells incubated for prolonged periods also displayed a reduced sensitivity to the lipolytic action of adrenocorticotrophic hormone. 5. During prolonged incubation there was no damage to the cells as judged by the retention of two soluble cytoplasmic enzymes, lactate dehydrogenase and malate dehydrogenase, within the cells.


Factors influencing the utilization of reduced nicotinamide adenine dinucleotide by pigeon heart mitochondria.


Effects of phenfromin and hypoglycin on gluconeogenesis of rat tissues.


The role of magnesium in the oxidation of external reduced nicotinamide adenine dinucleotide by rat liver mitochondria.


Aldosterone and corticosterone production by rat adrenal slices as a function of the time of incubation.


Accumulation of guanine nucleotides in the acid-soluble fraction of Bacillus subtilis inhibited by actinomycin D.


Histological studies of cortisone and A.C.T.H. treated rabbits after fracture.


Biochemical changes in the endoplasmic reticulum in liver injury.


Direct intracardiac extension of a carcinoma of the caecum.


A benign giant-cell synovioma in a cat.

A benign giant-cell synovioma in a cat.


Demonstration of the Golgi apparatus in cells in tissue culture.


Further isolations of reovirus type 3 from cases of Burkitt's lymphoma.


Case of encephalitis due to herpes simplex.


Spontaneous tumours in rats and mice.

Spontaneous tumours in rats and mice.


Effects of cortisol implants in the brainstem and ventral hippocampus on diurnal corticosteroid levels.


A study of skeletal maturation of hand and wrist and its relationship to nutrition.


The effect of acute haemorrhage in the dog and man on plasma-renin concentration. 1. The effect of acute haemorrhage on the plasma renin concentration was studied in the dog and man.2. Plasma-renin concentration was regularly increased after the larger bleeds; after the smaller haemorrhages plasma-renin concentration remained unchanged.3. The results are discussed in relation to current hypotheses concerning the control of renin and aldosterone secretion.


Hematologic phagocytosis in postmortem bone marrows of dengue hemorrhagic fever. (Hematologic phagocytosis in Thai hemorrhagic fever).


The myocardial succino-oxidase and myosin adenosine triphosphatase activities after coronary arterial infusion of histamine in dogs.


Respiratory activity of isolated mammalian nuclei. 3. The reduced nicotinamide adenine dinucleotide oxidase of rat liver nuclei and nucleoli.


Chicken gizzard myosin.

Chicken gizzard myosin.


Phosphorolysis of adenosine diphosphoribose.


Glycine reduction to acetate and ammonia: identification of ferredoxin and another low molecular weight acidic protein as components of the reductase system.


Demonstration of pancreatic insulomas by angiography.


Failure to diagnose Zollinger-Ellison syndrome by pancreatic arteriography. Report of a case.


Lymphangiography and endolymphatic radiotherapy in testicular tumours.


Observations on the surgical interruption of lymphatic pathways.


Granulomatous reactions induced by silica. I. Relationships between particles size and multinucleated giant cells.


Effect of cellular disruption on certain metabolic activities in Vibrio cholerae.


Co-ordination chemistry and membrane function with particular reference to the synapse and catecholamine transport.


Bronchiolar apocrine tumor.

Bronchiolar apocrine tumor.


Purification of a non-heme iron protein and other electron transport components from Azotobacter extracts.


Nitrogen base inhibition of yeast alcohol dehydrogenase.


Intracellular distribution of cathepsin B and cathepsin C in rat liver.


Selective hydrolysis of rat-liver lecithin by phospholipase C.


Virus dispersal by water.

Virus dispersal by water.


Influence of ACTH on convulsive activity in children. Electroencephalographic and clinical observations.


Amino acid and vitamin requirements of Bordetella pertussis.


Role of thymus in viral leukemogenesis in rats.


Some observations on the effect of mammary tumour agent on cells cultivated in vitro.


Histochemical studies of cholinesterases in Ciona intestinalis.


Phospholipid patterns of Periplaneta americana during embryogenesis.


Effects of cholinergic drugs on tunicate smooth muscle.


[Studies on pig erythrocyte lipids. I. Fractionation of erythrocyte lipids by the simple combination of silicic acid and cellulose column chromatography].


Quantitative analysis of urinary 17-oxogenic steroids using sodium metaperiodate oxidation.


The automated determination of NAD-coupled enzymes. II. Serum lactate dehydrogenase.


An enzymatic-spectrophotometric determination of pyruvic and lactic acid in blood. Methodologic aspects.


The mechanism of insulin action. The effect of insulin action. The effect of insulin on the allosteric properties on intracellular haemoglobin. 1. The results in three experimental series have suggested that a number of modifying factors must be taken into account in considering the allosteric behaviour of structurally bound intracellular, as distinct from free, haemoglobin. It seems that intracellular haemoglobin maintains its maximal internal stability during oxygen uptake and release by configurational changes which involve not only its acid-base-binding groups but also partial electron-transfer complexes with the stromal macro-molecular framework. Conversely, the affinity of intracellular haemoglobin for oxygen reflects the positive or negative electron pressures prevailing at the haemoglobin-stromal complexing sites.2. Active glucolysis in intact cells entails continuous poising of metabolic redox potential changes by the stromal material (in the same way as it entails continuous buffering of acid metabolic products). The consequent shifts in stromal redox potential and capacity are transmitted to haemoglobin and are reflected in a changing affinity for oxygen.3. The behaviour of the intracellular haemoglobin-stromal complex is further modified by extracellular electron donors and acceptors and by the capacity of the cell/extracellular interface to act as an electrochemical barrier between them. It is probably the last variable which is directly influenced by insulin.4. Two parameters have been introduced as empirical measures of the distinctive allosteric properties of cell-bound haemoglobin: (i) D-pH to represent the apparent cell/extracellular pH gradient; and (ii) D-O(2) to represent the volume of oxygen evolved when cells are lysed at a constant P(O2). Under a variety of experimental conditions insulin affected both these parameters.5. The insulin effects were consistent with a concept which rests on three main contentions. (i) Insulin has an immediate physical action on the cell/extracellular interface, altering the redox potential gradient (electron conductance) across the plasma membrane. (ii) Conversely, the formation of an insulin-membrane complex depends, among other factors, on the prevailing trans-membrane redox potential difference. (iii) The consequences of insulin-membrane interaction are both immediate and sustained, and, under appropriate conditions, they are reflected in the affinity of intracellular haemoglobin for oxygen.6. At a more general level it has been shown that in many respects haemoglobin behaves as a built-in intracellular redox indicator dye. Like most synthetic redox indicators, it is pH dependent.


Studies of adenovirus-SV40 hybrid viruses. IV. An adenovirus type 2 strain carrying the infectious SV40 genome.


Determining factor in the capacity of Rous sarcoma virus to induce tumors in mammals.


Mutants of bacteriophage T4 unable to cause breakdown of host DNA.


Biological polymers related to catechol: electron paramagnetic resonance and infrared studies of melanin, tannin, lignin, humic acid and hydroxyquinones.


Studies on polynucleotides. LIV. A further study of the reaction of nucleotide pyrophosphates with carboxylic acid anhydrides.


Genetic and non-genetic factors in chromatographic behaviour of poliovirus.


Protective effect of neonatal thymectomy on cystic disease induced in rats by Rous sarcoma virus.


Stereospecificity of certain soluble and particulate preparations of mitochondrial reduced nicotinamide-adenine dinucleotide dehydrogenase from beef heart.


Stimulation of steroid C-11-beta-hydroxylation in adrenal mitochondria by cyclic 3',5'-adenosine monophosphate.


Carcinoma of the ovary. A clinicopathological study of 86 autopsied cases with special reference to mode of spread.


Prevalence of enterovirus infection among healthy young children in Taipei area.


Granular cell tumors (myoblastomas) of the tracheobronchial tree.


Effect of vitamins in single dose on liver of mice on prolonged ethanol intake.


Effect of estrogens on blood clotting and plasmin systems.


Variations of tensile strength of human cortical bone with age.


The effects of aldosterone and angiotensin on renal function.


The direct renal action of angiotensin in the rabbit.


Relationship of plasma volume changes to resistance and capacitance vessel effects of sympathomimetic amines and angiotensin in man.


Susceptibility of turkey-poults to two avian leucosis strains.


Electron-microscope proof of virus particles in fowl myelocytomatosis.


Paralysis of the peroneal nerve in association with a plantar flexion inversion injury of the ankle.


An improved method for the radioisotopic labeling of poliovirus and vaccinia virus. SAM-TR-66-29.


Oxidation of malate by Mycobacterium phlei extracts.


Hepatoma induced by thorium dioxide (thorotrast). A case of hepatoma induced by thorium dioxide (Thorotrast) is reported. The literature concerning neoplasia associated with this agent is reviewed, with emphasis on the long latent period before the development of these tumours and the equally long latent period which preceded the recognition of their iatrogenic nature.In particular, this report is intended to illustrate the diagnosis of such tumours on radiological and histological findings, provided that one is aware of their existence and familiar with the distribution and appearance of thorium dioxide deposits in the tissues. Such a diagnosis can be made without a history of thorium dioxide administration, which often may not be available.


Inquiries into the myocardial enzyme activity in experimental myocarditis.


Histochemical inquiries into the conduction system of the heart in some hoofed animals.


Separation of 3-alpha- and 3-beta-hydroxysteroid oxidoreductases from the soluble fraction of rat liver by gel filtration.


The influence of endoxan, hydrocortisone and antistine on the metabolism of cells cultured in vitro.


Comparison of anaesthesia with innovar, halothane and methoxyflurane-nitrous oxide.


Islet cell tumors. Case report.

Islet cell tumors. Case report.


The behavior of thiamine pyrophosphatase, acid phosphatase and NADA-2-tetrazolium reductase in the spinal cord of mice intoxicated with benzene.


Adrenal blood flow response to adrenocorticotrophic hormone and other stimuli in the dog.


Preparation, characterization and determination of glycogen in rat ascites hepatomas.


Adenovirus complement-fixing antibody in atypical pneumonia and difference in antibody titer among adenovirus types used as antigen.


Studies on the pleomorphism of HVJ virons.


Electrophoretic separation of polyoma virus DNA from host cell DNA.


The reversion of guanidine inhibition of poliovirus synthesis.


Magnesium trisilicate increases virus transmission.


Some aspects of myasthenia gravis.

Some aspects of myasthenia gravis.


Treatment of severe myasthenia gravis with large doses of ACTH.


Cytochrome oxidase and its derivatives. VI. The CO combining capacity of cytochrome C oxidase.


Experimental studies on bacteriocinogeneity in Clostridium perfringens type A. I. Isolation of bacteriocines and their antibacterial spectrum.


Respiratory chain and phosphorylation site of the sonicated membrane fragments of Micrococcus lysodeikticus.


Purification and properties of a pyrophosphatase from rat liver microsomes capable of catalyzing the hydrolysis of UDP-glucuronic acid.


Reproduction of the viruses isolated from cases of infectious hepatitis on Detroit-6(VA) cell line.


Lipid of cancer tissues. I. Lipid composition of Shionogi carcinoma 115 and Nakahara-Fukuoka sarcoma.


Angiofibroma of the nasopharynx.

Angiofibroma of the nasopharynx.


On the ligand field of iron in ferredoxin from spinach chloroplasts and related nonheme iron enzymes.


Local complications of thiopentone injection.


Action of proflavine on the development of Sendai virus in HEp 2 cells.


Thiosemicarbazone-resistant rabbitpox virus.


Fate of intravenously administered interferon and the distribution of interferon during virus infections in mice.


An enzymatic fluorometric micromethod for the determination of glycerol.


Clinical notes on feline respiratory viral disease.


Effects of dilute acids on the chemical and immunochemical properties of human orosomucoid.


Theoretical dental technology for apprentices' refractory materials.


Granular cell myoblastoma of the tongue: report of case.


Protein synthesis by cell-free extracts of Crithidia oncopelti.


Studies on the oxidative metabolism of Moniliformis dubius (Acanthocephala).


Antigens of Bordetella pertussis. IV. Effect of heat, merthiolate, and formaldehyde on histamine-sensitizing factor and protective activity of soluble extracts from Bordetella pertussi.s. Munoz, J. (Rocky Mountain Laboratory, Hamilton, Mont.), and B. M. Hestekin. Antigens of Bordetella pertussis. IV. Effect of heat, Merthiolate, and formaldehyde on histamine-sensitizing factor and protective activity of soluble extracts from Bordetella pertussis. J. Bacteriol. 91:2175-2179. 1966.-Both histamine-sensitizing and protective activities of soluble preparations from Bordetella pertussis cells are destroyed by heating at 80 C for 0.5 hr. The histamine-sensitizing activity appeared to be more susceptible to inactivation by heat than the protective activity. Formaldehyde in a final concentration of 0.5% rapidly diminished the histamine-sensitizing ability of saline extract (SE) held at 37 C. The protective activity was clearly more resistant to inactivation by formaldehyde at similar temperature. The inactivating action of formaldehyde was slower when the concentration of SE was increased or when the mixture was kept at 2 to 5 C. Merthiolate in a final concentration of 1:10,000 had no demonstrable deleterious effects on either protective or histamine-sensitizing activity of SE.


Production of interferon by alveolar macrophages. Acton, Jean D. (Bowman Gray School of Medicine, Winston-Salem, N.C.), and Quentin N. Myrvik. Production of interferon by alveolar macrophages. J. Bacteriol. 91:2300-2304. 1966.-Rabbit alveolar macrophages inoculated with parainfluenza-3 virus in vitro produce a viral inhibitor which possesses the properties of interferon. The interferon is nondialyzable, is stable at pH 4, is not sedimented at 100,000 x g, exhibits species specificity, and can passively protect other alveolar macrophages from infection with virulent rabbitpox virus. The possible significance of alveolar macrophage-produced interferon is discussed.


Urea and thermal mutants of poliovirus.

Urea and thermal mutants of poliovirus. Hallum, J. V. (University of Pittsburgh, Pittsburgh, Pa.), and J. S. Youngner. Urea and thermal mutants of poliovirus. J. Bacteriol. 91:2305-2308. 1966.-Thermally selected mutants of poliovirus which differed in sensitivity to heating at 50 C were inactivated with urea. With 3 m urea, urea resistance paralleled thermal resistance. In contrast, when urea-resistant mutants were selected from thermosensitive, urea-sensitive, wild-type poliovirus, the 50 C sensitivity of the selected mutants did not change. These findings indicate a lack of covariance of the urea and the 50 C-inactivation markers of poliovirus.


Differential inhibitory effects of actinomycin D among strains of poliovirus. Schaffer, Frederick L. (University of California, Berkeley), and Marjorie Gordon. Differential inhibitory effects of actinomycin D among strains of poliovirus. J. Bacteriol. 91:2309-2316. 1966.-Actinomycin D exerted a differential effect on the ability of strains of poliovirus to replicate in HeLa cells. LSc-2ab was studied as an example of a strain markedly inhibited by actinomycin; MEF(1) served as a control strain with minimal inhibition. The effect was noted at an actinomycin concentration of 0.1 mug/ml, but 2.5 mug/ml was used for most studies. Variability in the effect was attributed, in part, to physiological factors. Actinomycin was effective when present during the first 2 hr of LSc infection, but had little effect if present at later times. It did not block adsorption or initiation of ecilpse. It did block synthesis of ribonucleic acid in LSc-infected cells. Several possible modes of action are discussed, the most attractive being that actinomycin blocks synthesis of some cell component, the concentration of which is more critical for replication of some poliovirus strains than others.


Replication of mengovirus. I. Effect on synthesis of macromolecules by host cell. Plagemann, Peter G. W. (Western Reserve University, Cleveland, Ohio), and H. Earle Swim. Replication of mengovirus. I. Effect on synthesis of macromolecules by host cell. J. Bacteriol. 91:2317-2326. 1966.-The replication of mengovirus was studied in two strains of Novikoff (rat) hepatoma cells propagated in vitro. The replicative cycle in both strains required 6.5 to 7 hr. Infection resulted in a marked depression of ribonucleic acid (RNA) and protein synthesis by strain N1S1-63. Inhibition of RNA synthesis was reflected by a decrease in the deoxyribonucleic acid (DNA)-dependent RNA polymerase activity of isolated nuclei. Mengovirus had no effect on either protein or RNA synthesis or on the DNA-dependent RNA polymerase activity of a second strain, N1S1-67. The time course of viral-induced synthesis of RNA by cells was studied in cells treated with actinomycin D. It was first detectable between 2.5 and 3 hr after infection and continued until 6.5 to 7 hr. The formation of mature virus was estimated biochemically by measuring the amount of RNA synthesized as a result of viral infection which was resistant to degradation by ribonuclease in the presence of deoxycholate. Approximately 70% of the deoxycholate-ribonuclease-resistant RNA was located in mature virus, and the remainder was double-stranded. The formation of mature virus began about 45 min after viral-directed (actinomycin-resistant) synthesis of RNA was detectable in the cell, and only about 18 to 20% of the total RNA synthesized was incorporated into virus. Release of virus from cells began about 1 hr after maturation was first detectable. Release of virus from cells was accompanied by a loss of a large proportion of their cytoplasmic RNA and protein.


Replication of mengovirus. II. General properties of the viral-induced ribonucleic acid polymerase. Plagemann, Peter G. W. (Western Reserve University, Cleveland, Ohio), and H. Earle Swim. Replication of mengovirus. II. General properties of the viral-induced ribonucleic acid polymerase. J. Bacteriol. 91:2327-2332. 1966.-Mengovirus induces the appearance of a ribonucleic acid (RNA) polymerase activity in Novikoff hepatoma cells which is readily distinguished from the deoxyribonucleic acid (DNA)-dependent RNA polymerase since it is not inhibited by actinomycin D or deoxyribonuclease, but is inhibited by ammonium sulfate, and is stable at -17 C. The incorporation of uridine into RNA by infected cells in the presence of actinomycin D does not reflect the viral polymerase activity as measured in cell-free preparations. The viral-induced RNA polymerase is produced in a biphasic fashion. Puromycin inhibits the production of viral polymerase, and in its presence the enzyme appears to be unstable between 4 and 6 hr. Puromycin also prevents the secondary rise in polymerase which begins at the end of replicative cycle. Under these conditions, however, the polymerase appears to be stable. The overall data indicated that some unspecified process is responsible for the apparent instability of viral-induced RNA polymerase between 4 and 6 hr and that it becomes inoperative toward the end of the replicative cycle.


Presence and persistence of foot-and-mouth disease virus in bovine skin. Gailiunas, Peter (Plum Island Animal Disease Laboratory, Greenport, N. Y.), and George E. Cottral. Presence and persistence of foot-and-mouth disease virus in bovine skin. J. Bacteriol. 91:2333-2338. 1966.-This study established that the seven known antigenic types of foot-and-mouth disease virus (FMDV) have consistent affinity to all areas of bovine skin, even though gross cutaneous lesions usually are found only in the pedal area. Considerable amounts of FMDV were present in skin of 13 different body areas, irrespective of the presence of hair. All skin specimens from the trunk of 50 experimentally infected steers, necropsied from 12 hr to 7 days postinoculation (DPI), contained FMDV in the dermal and epidermal tissues. In skins of some steers, FMDV persisted for as long as 5 days after cessation of viremia. The highest average virus titer, 10(3.6) plaque-forming units (PFU) per g of skin, was found at 2 DPI. Some areas of the trunk and extremities had titers of approximately 10(5.0) PFU per g of skin. Characteristic gross lesions were not observed in sampling areas. The present observations have epizootiological importance for hides offered in international trade, because FMDV localized intracutaneously is more difficult to inactivate than virus adhering to hide surfaces.


Growth characteristics of two rhinovirus strains in WI-26 and monkey kidney cells. Haff, R. F. (Smith Kline & French Laboratories, Philadelphia, Pa.), B. Wohlsen, E. E. Force, and R. C. Stewart. Growth characteristics of two rhinovirus strains in WI-26 and monkey kidney cells. J. Bacteriol. 91:2339-2342. 1966.-Viruses with 1059 and HGP serotype and with human and monkey host range characteristics, respectively, were employed. Adsorption kinetics of the 1059 and HGP strains to WI-26 cells, and HGP to Green African monkey kidney cells (MKC), were similar. Fifty per cent of the virus was adsorbed to cell monolayers within 10 min; adsorption was essentially complete by 2 hr. The 1059 strain failed to adsorb to MKC, at least to an appreciable extent. Lack of receptors for adsorption of 1059 accounts for the inability of this cell to support multiplication of the virus. It is probable that MKC are refractory to infection with other H strains of rhinovirus for the same reason. Single-step multiplication cycles have been described for the HGP strain in WI-26 and MKC cultures and for the 1059 strain in WI-26 cells. In both cells, HGP exhibited a latent period of 7 hr. Increase of intracellular and cell-associated virus appeared somewhat prior to that of extracellular virus. Maximal titers were attained by 9 to 10 hr. In contrast, initial increase of 1059 in WI-26 cells occurred after 10 hr. Titer rose to peak level 15 hr after infection. Yield of 1059 in WI-26 cells was also fivefold lower than that of HGP in either cell system.


Inapparent viral infection of cells in vitro. 3. Manifestations of infection of L mouse cells by Japanese encephalitis virus. Dubbs, D. R. (University of Minnesota, Minneapolis), and W. F. Scherer. Inapparent viral infection of cells in vitro. III. Manifestations of infection of L mouse cells by Japanese encephalitis virus. J. Bacteriol. 91:2349-2355. 1966.-Nine strains of Japanese encephalitis (JE) virus were propagated serially in cultures of L cells reaching titers of 10(3.5) to 10(6.3). Although cytopathic effects were not seen in cultures of contiguous L cells after infection with JE virus, cell growth was inhibited. Moreover, cell destruction was readily apparent in infected cultures of sparse, noncontiguous L cells. Differences in the size of cell population of infected and noninfected cultures (i) occurred despite only 0.2 to 3.5% of the cells in infected cultures being associated with infectious virus, (ii) were greater in actively growing cultures than in those kept in maintenance media, and (iii) were probably in part related to an interferon produced in infected cultures.


Granular cell myoblastoma of the vermiform appendix.


Sudden unexpected death in infancy and viral infection.


Effects of norethandrolone on cortisone-induced pitiutary-adrenal suppression in the rat.


Effect of niamid and benzquinamide (Quantril) on the release of corticotrophin in the rat.


Studies on potassium dependent phosphatase; its distribution and properties.


Semiquinoid flavins adsorbed on Florisil.


Effects of melatonin and related compounds on the release of glycerol from rat adipose tissue in vitro.


Crystal-induced inflammation in canine joints. I. An experimental model with quantification of the host response. Injection of sodium urate or calcium pyrophosphate crystals into the stifle joints of anesthetized dogs almost invariably induced an acute exudative response. This response was quantified by serial measurements of intra-articular pressure, pH and leukocyte concentration. Pressure rose progressively and reflected intra-articular volume increase. The hydrogen ion concentration increased as the reaction progressed and correlated in a given exudate with the leukocyte concentration. Analysis of sequential physiologic and biochemical changes occurring in this model of crystal-induced inflammation may provide insight into the mechanisms of acute gouty arthritis in man.


Infectious diseases, today and tomorrow.


Retroperitoneal chemodectoma.

Retroperitoneal chemodectoma.


Influence of age on induction with urethan of hepatomas and other tumors in infant mice.


Interrelations between glucose loading and glucose-6-phosphatase activity in liver of normal and alloxan-diabetic dogs.


Tumor induction in transplanted tooth buds infected with polyoma virus.


Rhabdomyoma, a rare, benign neoplasm of skeletal muscle.


Quantitative precipitation of various 3-beta-hydroxysterols with digitonin.


Serological and biological studies on porcine enteroviruses isolated in Canada.


Melanocyte-stimulating hormone activity of synthetic MSH and ACTH peptides, in vivo and in vitro.


On the cellular site of corticotrophin production.


Serotypic relationships among rhinoviruses.


Filter paper disc method of collecting whole blood for serologic studies in children.


Accumulation and elimination of poliovirus by the eastern oyster.


Viruses and disease: studies in a children's home.


Minerals and the ruminant with special reference to the discovery of swayback in the goat.


Lactic acid racemization in Cl. butylicum: 18O exchange studies.


Interaction of heme A and polylysine.

Interaction of heme A and polylysine.


Unusual kinetic properties of a DPN-linked lactate dehydrogenase from Butyribacterium rettgeri.


Chemistry of micrococcin P. X. Proton magnetic resonance spectrum of dimethyl micrococcinate, and the probable mode of biosynthesis of micrococcinic acid.


Masculinizing lipoid cell tumor of the ovary. Report of a case.


Adenoid-basal carcinoma of the cervix.

Adenoid-basal carcinoma of the cervix.


Plasma 17-hydroxycorticosteroid patterns; tends in toxemia.


Carcinogenic effect of N-hydroxy-N-2-fluorenylacetamide, 2',4'-dimethylacetanilide, and 2',4'6'-trimethylacetanilide on liver in suckling mice.


Purification and biophysical properties of rhinoviruses.


Cyclic fluctuations in I-131 content of thyroid glands of cats and monkeys.


Occurrence of adenovirus-SV40 hybrids among monkey kidney cell adapted strains of adenovirus.


Gradient plate technique applied to the study of antiviral substances.


Influence of the pituitary-adrenal axis on development of the rat foetal thymus.


Inhibitory effect of ACTH and related peptides on extinction of conditioned avoidance behavior in rats.


Interferon production by human leucocytes in vitro. Reduced levels in lymphatic leukemia.


Transplantation of pituitary "mammotropic" tumor (MtT.F4) from Fisher to Sprague-Dawley rats.


Cytomegalovirus infection. Viral ultrastructure with particular reference to the relationship of lysosomes to cytoplasmic inclusions.


Mitochondrial particles resolved for ion translocation. I. Preparation and properties of a particle coupled only at phosphorylation site 3 of the electron transfer chain.


Effect of p-hydroxypropiophenone on fibrosis induced by carbon tetrachloride in mice.


The restoration of DPNH oxidase activity by coenzyme Q (ubiquinone).


Phosphorylated intermediates of terpene biosynthesis in Pinus radiata.


NADH oxidase system of Agrobacterium tumefaciens.


The adsorption of metal from HF-HC10-4 mixtures on some ion exchange papers.


Effect of growth hormone on enzyme activities in the hypophysectomized rat.


Modified anterior tibial compartment syndrome.


Intrauterine contraceptive device and ovarian thecoma.


Biochemical studies in a patient with hyperinsulinism.


Changes in the carotid pulse which occur with age and hypertension.


On the theory of silicosis. I. Coesite.

On the theory of silicosis. I. Coesite.


Corneal xanthomas in childhood.

Corneal xanthomas in childhood.


Mixed tumours of the submandibular gland.


Cylindroma of external ear.

Cylindroma of external ear.


Electron microscopic and histochemical studies on vacuolar alteration of proximal convoluted tubules of rat kidney after hypertonic sucrose administration.


Alveolar clearance: its relation to lesions of the respiratory bronchiole.


Prevention of indwelling urinary catheter infections.


Electrophysiological bracing in a radial nerve compression injury.


Bronchogenic carcinoma and the peripheral neuromyopathies.


Lack of relationship between infection with avian leukosis virus and the presence of COFAL antigen in chick embryos.


Impairment of EMC viral infectivity by cell-associated ribonuclease.


The mechanism of interference between an avian leukosis virus and Rous sarcoma virus. I. Establishment of interference.


The mechanism of interference between an avian leukosis virus and Rous sarcoma virus. II. Early steps of infection by RSV of cells under conditions of interference.


Structural components of vesicular stomatitis virus.


Comparative study of experimental atherosclerosis production in white rats.


Presence of particles with the morphology of viruses of the avian leukosis complex in meningeal tumors induced in dogs by Rous sarcoma virus.


Cytomegaly in a 76-year old man with chronic lymphatic leukemia.


Respiration and oxidative phosphorylation of mitochondria in regenerating liver of rats.


Mechanism of increase in respiration intensity during embryonic development of fish.


The problem of malignancy of the carotid body and similar structures.


Cell strains obtained from tumors induced by SV40 virus.


Reproduction of Rous virus in mouse embryonic tissue in vitro.


Effect of iron deficiency on the gastrointestinal tract of the rat.


Fractionation of Japanese encephalitis virus by column chromatography with calcium phosphate.


The influence of deuterium oxide and organic solvents on the interaction of respiratory chain components.


Inhibition of glutamic dehydrogenase by N-1-alkylnicotinamide chlorides.


Electron transport systems of yeast. 3. Preparation and properties of cytochrome oxidase.


Phosphotransferase activities of kidney glucose 6-phosphatase.


Preparation and some properties of yeast mitochondria.


Sodium-potassium-activated adenosine triphosphatase of Electrophorus electric organ. 3. An associated potassium-activated neutral phosphatase.


The effect of age and neurological disease on the ankle jerk.


Spheromembranous degeneration of muscle induced by vincristine.


Infectious bovine rhinotracheitis--clinical, pathological, and virological aspects.


The effect of central nervous system stimulation on erythropoiesis in rats with chronically implanted electrodes.


Peripheral neuropathy in rats produced by acrylamide.


Hydrolase cytochemistry of retrograde neuronal degeneration in feline lateral geniculate body. With observations on the identification of multiple forms of neural hydrolases having overlapping substrate affinities.


Antigenic structure of chemically-induced hepatomas.


Pathogenicity and oncogenicity of Rous sarcoma virus for mammals.


Oxidative enzymes responsible for the conversion of 3,4-dihydroxyphenylalanine to melanin in the small intestine of rodents.


Cytochemistry of cytoplasmic and intranuclear inclusions induced by bovine parainfluenza 3 virus (SF-4) in human cell cultures.


Variation of localization of AMPase and ATPase activities at the plasma membrane of human prostatic epithelial cells: an electron microscopic study.


Pseudomucinous cystadenocarcinoma of the ovary associated with pregnancy. Case report.


Clinicopathologic conference.

Clinicopathologic conference.


Some observations on the influence of insulin, prednisolone and free amino acids on the glucose metabolism in cultivated liver tissue.


Cytochemistry of foetal chicken liver cultivated. In vitro.


Cytochemical and enzymatic disorders of lymphatic nodes in reticulosarcomatosis and malignant lymphogranulomatosis.


Electron histochemical investigations of the effect of thioacetamide on the regenerating rat liver cell.


Prelimnary report on the therapeutic use of a synthetic corticotrophin.


Cytochemical studies on the relation between the Golgi structures and elaioplasts in Malva neglecta L.


Effect of phosphates upon experimental skin calcinosis.


Substrate and product inhibition of rabbit muscle lactic dehydrogenase heart (H4) and muscle (M4) isozymes.


Properties of dipeptidyl arylamidase I of the pituitary. Chloride and sulfhydryl activation of seryltyrosyl-beta-naphthylamide hydrolysis.


Localization and partial characterization of an aldehydic component in tropocollagen.


Further evidence for the accumulation of a hydroxyproline-deficient, collagenase-degradable protein during collagen biosynthesis in vitro.


Action of the murine toxin of Pasteurella pestis on enzyme complexes of the electron transport system.


Cofactors and erythrocyte glycolytic capacity.


D-fructose 1-phosphate kinase, a new enzyme instrumental in the metabolism of D-fructose.


Studies on the electron transfer system. LXVI. Effect of diphosphopyridine nucleotide deficiency on respiration, respiratory control, and phosphorylation in mitochondria.


Constitutive inorganic pyrophosphatase of Escherichia coli. II. Nature and binding of active substrate and the role of magnesium.


Electron spin resonance studies of the denaturation of oxy- and methemoglobin. Spectra and solvent interactions.


The enzymatic reduction of nicotinamide N-oxide.


Studies of carcinogenesis by avian sarcoma viruses. II. Virus-induced increase in hyaluronic acid synthetase in chicken fibroblasts.


Influence of glucosylation of deoxyribonucleic acid on hydrolysis by deoxyribonucleases of Escherichia coli.


Glycopeptides from bovine aorta glycoprotein.


The chondroitin 4-sulfate-protein linkage.


Multiple primary mixed tumors of the salivary gland. A case report.


Decarboxylation of 2-keto fatty acids by brain. An enzyme system consisting of washed acetone powder of pig brain, adenosine triphosphate, nicotinamide adenine dinucleotide, magnesium, fumaric acid, and ascorbic acid was found to catalyze the oxidative decarboxylation of 2-ketostearic acid. The products were carbon dioxide and heptadecanoic acid. Washing the enzyme powder with ethylene-diaminetetraacetate and other chelating agents destroyed the activity. Further properties of the enzyme system are described. It is believed that this enzyme system accounts in part for the 1-carbon degradation route for brain fatty acids.


Synthesis and characterization of 1- and 2-monoglycerides of anteiso fatty acids. The branched-chain fatty acids D-(+)-12-methyltetradecanoic acid (C(15) anteiso) and D-(+)-14-methylhexadecanoic acid (C(17) anteiso) were isolated from the lipids of Listeria monocytogenes and their 1- and 2-monoglycerides were prepared. Reaction intermediates and products were purified without isomerization by column chromatography. Thin-layer chromatography on Florisil impregnated with boric acid and nuclear magnetic resonance were used in characterizing the 1- and 2-monoglycerides. The value of the latter method for analyzing glyceride structure is discussed.


Electron microscopy of the lemmocyte in peripheral nerve tumors (neurolemmomas).


Pancreatoduodenectomy for islet-cell tumor of the pancreas in infancy and childhood. Case report with five-year survival.


Composition and properties of a cell-membrane fraction from the polymorphonuclear leucocyte. 1. The composition of a vesicular cell-membrane fraction from leucocytes has been studied. The bulk of the mass is accounted for as protein and lipid. A small amount of carbohydrate, including some N-acetylneuraminic acid, is present. The phospholipid/cholesterol molar ratio is 1.4 and differs from that for the whole cell. 2. Labile phosphorus groups are present in the membrane but the analysis is complicated by the presence of phosphorus occluded in the membrane vesicles. 3. Leucocidin does not change the gross composition of the membranes or alter the amount or reactivity of the phosphorus compounds. 4. The cell-membrane fraction has considerable avidity for an impurity present in commercial [(32)P]orthophosphate. When this is removed [(32)P]orthophosphate or [(32)P]ATP does not label the membrane. 5. The presence of an NADH(2)-cytochrome c oxidoreductase and an alkaline phosphatase is described. The adenosine-triphosphatase activity of the membrane has not been found to depend on the presence of Na(+) or K(+).


Adenovirus infections in servicemen in Finland.


Role of thyroid hormones in lipolysis.

Role of thyroid hormones in lipolysis.


Adrenal-dependent circulatory responses to angiotensin in the cat.


Effects of angiotensin, epinephrine, norepinephrine, and vasopressin on venous return.


Fluorescence-inducing corticosteroids in peripheral blood of newborn infants: response to ACTH and operation.


Surgical applications of ethiodol with chlorophyll in lymphangiography: histopathologic, radiographic and clinical disadvantages in 36 cases.


Adenocarcinomas of the anal canal and peri-anal tissues.


Mesenchymal hamartoma of the liver. Case report and literature review.


Sebaceous lesions of the parotid. Report of two cases.


p-Hydroxypropiophenone vs CCL4-induced injury.


Stable phosphorylating submitochondrial particles from baker's yeast.


Biosynthesis of NAD and nicotinic acid by Clostridium butylicum.


Immunologic responsiveness and the induction of experimental neoplasms.


The separation of some pairs of allylic-propenylic isomers by TLC on silica impregnated with silver nitrate.


Some observations on the diagnosis of rectal gonorrhoea in both sexes using a selective culture medium.


Agar derivatives for gel electrophoresis requiring reduced endosmotic liquid flow.


Temperature dependence of the electrophoretic mobility of cells and quartz particles.


Adrenocortical and pituitary function following long-term prednisolone treatment.


Carotid vascular receptors and control of adrenal corticosteroid secretion.


Effect of adrenal demedullation on vascular responses after immunological sympathectomy.


Tissue concentrations of manganese and adrenal function.


Effects of sinoaortic denervation on renal and adrenal hypertension.


Syntheses of peptides related to N-terminal structure of corticotropin. VI. The synthesis of ser 1- and gly 1-decapeptides corresponding to the first ten-amino-acid sequence of corticotropin.


Studies of the DPNH-cytochrome b segment of the respiratory chain of baker's yeast.


Nephroblastoma (Wilms's tumor): urographic diagnosis and prognosis.


A physiological and simple approach to lymphography. An experimental study.


Primary carcinoma of the liver.

Primary carcinoma of the liver.


Study of the thoracico-abdominal lymphatic system. I. Lymph node perfusion technique.


Study of the thoracico-abdominal lymphatic system. II. Lymph node perfusion by overfill technique.


The specific cleavage of tyrosyl-peptide bonds by electrolytic oxidation.


The stoichiometry and absorption spectra of components a and a-3 in cytochrome c oxidase.


Effect of adenylic acid on the regulatory nicotinamide-adenine dinucleotide specific isocitrate dehydrogenase.


Brain tumors of diverse germinal origin arising in juxtaposition. Report of three cases.


Steroids in human skin and hairs. I. Zimmermann chromogens in the skin of normal and of hirsute women.


Steroids in human skin and hairs. 3. Cholesterol in the skin and hairs of normal and hirsute women, and in the hairs of normal men.


Myoepithelial cells in carcinoma of human breast.


Electron microscopic evaluation of the pathogenesis of porcine polioencephalomyelitis.


The cytopathology of swine pox in the skin of swine.


The hydrolysis of unimolecular films of P32-labelled lecithin, phosphatidylethanolamine and phosphatidylinositol with phospholipase A (Naja naja venom).


Heat-sensitive deoxyribonuclease activity in cells infected with herpes simplex virus.


Double infection of newborn Syrian hamsters with simian virus 40 and human adenovirus 12.


Sucrose utilization by Zymomonas mobilis: formation of a levan. 1. Molar growth-yield coefficients of Zymomonas mobilis for glucose, fructose, glucose plus fructose, and sucrose are reported. Yield coefficients for sucrose are appreciably lower than those for the equivalent concentrations of glucose plus fructose. 2. Only 2.6% of [U-(14)C]glucose supplied in the growth medium is incorporated into cell substance by Z. mobilis utilizing glucose as the energy source. 3. During growth on sucrose a levan is formed. It has been characterized and shown to resemble other bacterial levans. 4. Levan formation from sucrose could be demonstrated with both washed cell suspensions and cell extracts of Z. mobilis. 5. Sucrose phosphorylase could not be demonstrated in extracts of the organism.