The submitochondrial localization of monoamine oxidase. An enzymatic marker for the outer membrane of rat liver mitochondria. Controlled osmotic lysis (water-washing) of rat liver mitochondria results in a mixed population of small vesicles derived mainly from the outer mitochondrial membrane and of larger bodies containing a few cristae derived from the inner membrane. These elements have been separated on Ficoll and sucrose gradients. The small vesicles were rich in monoamine oxidase, and the large bodies were rich in cytochrome oxidase. Separation of the inner and outer membranes has also been accomplished by treating mitochondria with digitonin in an isotonic medium and fractionating the treated mitochondria by differential centrifugation. Treatment with low digitonin concentrations released monoamine oxidase activity from low speed mitochondrial pellets, and this release of enzymatic activity was correlated with the loss of the outer membrane as seen in the electron microscope. The low speed mitochondrial pellet contained most of the cytochrome oxidase and malate dehydrogenase activities of the intact mitochondria, while the monoamine oxidase activity could be recovered in the form of small vesicles by high speed centrifugation of the low speed supernatant. The results indicate that monoamine oxidase is found only in the outer mitochondrial membrane and that cytochrome oxidase is found only in the inner membrane. Digitonin treatment released more monoamine oxidase than cytochrome oxidase from sonic particles, thus indicating that digitonin preferentially degrades the outer mitochondrial membrane.


Foot-and-mouth disease in Myocastor coypus.


Oxidative and glycolytic recovery metabolism in muscle. The average degree of reduction of mitochondrial NAD has been measured in the intact toad sartorius by a fluorometric technique. It has been shown that cytoplasmic NADH does not interfere materially with these measurements. The percentage reduction of this respiratory coenzyme has been determined in a number of physiological steady states which are well correlated with fluorometrically determined levels of NADH in suspensions of mitochondria from the hind leg musculature of the toad. In addition, these findings are closely comparable to similar, spectrophotometric measurements on mitochondria from other sources. In the presence of an adequate O(2) level a single twitch produces a decrease in fluorescence from the resting steady state which is followed by a slow return to the base line condition. This cycle indicates the intensity and the time course of the oxidative recovery metabolism. The area under this curve is directly related to the number of twitches up to three or four. Greater activity produces a curtailment of oxidative recovery due to glycolysis. In the presence of iodoacetate the linear relation holds for five to seven twitches. At still higher levels of activity a curtailment of the change in NAD level sets in, probably due to the removal of AMP by catabolic reactions.


Automated determination of rat adrenal ascorbic acid in the bioassay of corticotropin.


Determination of 3-beta-hydroxysteroid dehydrogenase system in microgram samples of tissue, quantitative histologic distribution of the enzyme in the rat adrenal and effects of adrenocorticotropin.


The role of membrane phosphoglycerate kinase in the control of glycolytic rate by active cation transport in human red blood cells. When the internal Na of human red cells is raised, both K influx and lactate production increase and become more sensitive to the inhibitory action of ouabain. This occurs with either glucose or purine nucleoside as substrate. Fresh whole hemolysates enriched with Na and Mg will convert intermediates above the triose phosphate dehydrogenase step to lactate at a rate which is slowed by ouabain. Intermediates beyond the phosphoglycerate kinase step (PGK) are metabolized at a very rapid rate which is not affected by ouabain. No metabolic effects of ouabain were found in ghost-free hemolysates. Hemoglobin-free ghosts were shown to have both triose phosphate dehydrogenase and PGK activity. The rate of this two-enzyme sequence was found to be a function of the ADP concentration, being maximal when ADP > 0.35 mM. Initial addition of ATP to the ghost system rendered the forward rate of the sequence sensitive to the inhibitory action of ouabain. When the sequence was run in reverse, no inhibitory effect of ouabain could be demonstrated. It is concluded that membrane PGK is a point at which the Na-K transport system can influence the metabolic rate and that this action is possibly exerted via a compartmentalized form of ADP which is an immediate substrate for the ghost PGK.


Intercellular bridges between mammalian cells in culture.


Prevention of virus-induced neoplasms in mice through passive transfer of immunity by sensitized syngeneic lymphoid cells.


The interaction of the free radical of 5-methylphenazinium methyl sulfate with the light-induced free radical of Rhodospirillum rubrum chromatophores.


Haptoglobin 2-1 Bellevue, a haptoglobin beta-chain mutant.


Studies of adenovirus-SV40 hybrid viruses, VI. Differential effects of interferon on SV40 and adenovirus T antigen formation in cells infected with SV40 virus, adenoviruses, and adenovirus-SV40 hybrid viruses.


Isolation and properties of the helical nucleocapsid of the parainfluenza virus SV5.


The results of treatment in patients with cultures resistant to streptomycin, isoniazid and PAS: a five-year follow-up.


Inhibition of mitochondrial malate dehydrogenase by citrate.


Allosteric fine control of citrate synthase in Escherichia coli.


A comparison of microsomal (Na+ + K+)-ATPase with K+-acetylphosphatase.


Kinetics of NADH oxidation by pigeon-heart mitochondria.


The hydrolysis of monophosphoinositide by extracts of brain.


Specific action of prolactin on plasma sodium levels in hypophysectomized Poecilia latipinna (Teleostei).


Interrelations of selected measurements of the face and body in pre-adolescent and adolescent girls.


Hypersensitivity to peptide fragments.

Hypersensitivity to peptide fragments.


Selective extraction of polyoma DNA from infected mouse cell cultures.


Effect of the components parts of nicotinamide adenine dinucleotide (NAD) as inhibitors of lactic dehydrogenase.


Thiosemicarbazones derived from indanedione-1,3.


Action of angiotensin II upon renal vascular resistance.


Examination of structural effect on thin-layer chromatographic multiple spot formation of sympathomimetic amines in the presence of hydrochloric acid.


Cytomegalovirus infection after renal allotransplantation.


Epidermodysplasia verruciformis and epidermoid carcinoma. Electron microscopic observations.


Virus-like particles in a patient with chronic lymphocytic leukemia.


Viral etiology of Dawson's encephalitis.


Diphosphopyridine nucleotide: a cofactor for the polynucleotide-joining enzyme from Escherichia coli.


Regions containing repeating base-pairs in DNA from some oncogenic  and nononcogenic animal viruses.


A specific enzyme defect in gout associated with overproduction of uric acid.


Characterization of a polyisoprenoid compound functional in O-antigen biosynthesis.


Enzymatic joining of DNA strands: a novel reaction of diphosphopyridine nucleotide.


An improved ion-exchange procedure for the chromatography of the metabolites of mevalonate.


Influence of high renin substrate levels on renin-angiotensin system in pregnancy.


Chemotherapy of cerebral gliomas with intra-carotid methotrexate-infusion.


A gas-flow system for control of the temperature of an electron paramagnetic resonance sample at temperatures between 80 and 273 degrees Kelvin.


Survival of poliovirus in flowing turbid seawater treated with ultraviolet light. The effectiveness of a model ultraviolet (UV) radiation unit for treating flowing turbid seawater contaminated with poliovirus was determined. At a turbidity of 70 ppm, the observed survival ratios ranged from 1.9 x 10(-3) (99.81% reduction) to 1.5 x 10(-4) (99.98% reduction) at flow rates ranging from 25 to 15 liters/min; no virus was recovered at flow rates of 10 and 5 liters/min. At a turbidity of 240 ppm, the observed survival ratios ranged from 3.2 x 10(-2) (96.80% reduction) to 2.1 x 10(-4) (99.98% reduction) at flow rates ranging from 25 to 5 liters/min. As expected, turbidity had an adverse influence on the effectiveness of UV radiation; however, by adjusting the flow rate of the seawater through the treatment unit, adequate disinfection was shown to be predictable.


Inactivation of adenovirus and simian virus 40 tumorigenicity in hamsters by vaccine processing methods. The effectiveness of the adenovirus vaccine inactivation process in destroying the tumorigenic potential for hamsters of adenoviruses, simian virus 40 (SV-40), and adenovirus-SV-40 hybrids was studied. Baby hamsters injected with untreated virus and with samples subjected to the complete inactivation process and to portions of the process were observed for tumor development for periods in excess of 300 days. Over 20,000 hamsters were injected. From 1 to 7 hr of exposure to formaldehyde at a concentration of 0.031 m at 37 C was sufficient to destroy the tumorigenicity observed in the nontreated preparations. Since the inactivation process included 48 hr of exposure at 37 C to 0.031 m formaldehyde plus treatment with ultraviolet (UV) and with beta-propiolactone (BPL), it was concluded that the process has a large margin of safety. Adenovirus isolates free from tumorigenic potential are difficult, if not impossible, to obtain. Therefore, a proven inactivation process appears to provide the best assurance for obtaining adenovirus vaccines free from such potential. Data presented suggest that the tumorigenic property of the viruses studied might be independent of the infectivity of the preparation. The tumorigenic property was found to be highly susceptible to formaldehyde, but less sensitive to BPL or UV treatment. In contrast, treatment with UV or BPL decreased viral infectivity more readily than tumorigenicity. The three-stage inactivation process (formaldehyde, UV, and BPL) inactivated both tumorigenicity and infectivity.


Enumeration of Bacillus cereus in foods. For the enumeration of vegetative cells and spores of Bacillus cereus in foods, a mannitol-egg yolk-phenol red-agar has been developed which exploits the failure of B. cereus to dissimilate mannitol, and the ability of most strains to produce phospholipase C. When a high degree of selectivity was required, polymyxin B sulfate in a concentration of 10 ppm appeared to be the most effective selective additive. Useful characteristics for the identification of presumptive isolates of B. cereus were found to be: morphology, dissimilation of glucose mostly to acetyl methyl carbinol under anaerobic conditions, hydrolysis of starch and gelatin, reduction of nitrate, and growth on 0.25% chloral hydrate agar.


Statolon-induced resistance of mice to mengovirus. Mice receiving statolon intraperitoneally were 1,000 times more resistant to intraperitoneal challenge with mengovirus than were untreated controls. Protection was afforded when statolon was administered 1 day before or 1 day after intraperitoneal inoculation with the virus. No therapeutic effect was observed when treatment with statolon was delayed for 2 days or more after virus infection. Exposure of mice to a simulated space cabin environment did not increase their susceptibility to the lethal effects of mengovirus infection or eliminate the protective effect of statolon.


Studies of baker's yeast metabolism. I. Pyridine nucleotide reduction and oxygen utilization during alcohol oxidation.


Production of high-titer antibody in serum and ascitic fluid of hamsters for a variety of virus-induced tumor antigens. Methods not involving the use of viable tumors were developed for production of high-titer antibodies. The methods involved use of tumor homogenates. Homogenates from all tumor lines tested contained the characteristic tumor antigen. Attempts to isolate virus failed, and virus antigens could not be demonstrated. Immunization with tumor homogenates, in Freund's complete adjuvant, resulted in high antibody levels. None of the animals so immunized developed tumors.


Studies of baker's yeast metabolism. II. The role of adenine nucleotides and inorganic phosphate in the control of respiration during alcohol oxidation.


Studies of baker's yeast metabolism. 3. Oxidation of acetaldehyde.


Mutants partially deficient in alcohol dehydrogenase in Schizosaccharomyces pombe.


Inhibition of malate dehydrogenase by phenols and the influence of ring substituents on their inhibitory effectiveness.


The binding of NADH to liver alcohol dehydrogenase: a two step reaction.


The effect of the 57-Fe hyperfine interaction on the EPR spectrum of spinach ferredoxin.


AMP-sensitive fructose diphosphatase in higher plants: effects of carbon sources on the level of the enzyme.


Activator-inhibitor interactions in the adenosine diphosphate glucose pyrophosphorylase of Escherichia coli B.


The induction of NAD kinase by DDT in Triatoma infestans.


The effects of ions and freeze-thawing on supernatant and mitochondrial malate dehydrogenase.


Studies on the conformational changes of mitochondrial malate dehydrogenase in urea-phosphate solutions.


Excretion of phospholipids by men on fat-free diet.


Isolation of a series of Mengo virus variants differing in sensitivity to polyanions.


Composition of epiphyseal cartilage. V. Amino acid and amino sugar composition of epiphyseal cartilage of rachitic chicks; changes effected by vitamin D-3.


Excretion of phospholipids by men on high fat diets.


The pharmacology of the cardiac muscle of the great veins of the rat.


Urinary steroid spectrum by gas chromatography.


Bovine enteric cytopathogenic viruses. IV. Attempts to identify three prototype strains by neutralization tests with bacterial, bovine, and human reference antigens and antisera.


Autoimmunity and malignancy in New Zealand black mice.


The effect of ACTH on hormone excretion in patients with rheumatoid arthritis.


The measurement of hormones and their metabolites.


Fish pox in the roach (Rutilus rutilus L.).


Psychosomatic approach to bronchial asthma. A study of 50 adult asthmatics.


The influence of different levels of serum antibody on the susceptibility of rabbit oral mucosa to experimental herpes simplex virus infection.


U.V. stabilization of rescued poliovirus.


Isolation of protective antigen from Bordetella pertussis.


The effect of polyoma virus on differentiating multinucleated muscle fibers.


Contraction of hypotonically swollen mitochondria by oxidizable substrates.


Clostridium perfringens polynucleotide phosphorylase: two molecular species.


Stepwise degradation of P32-labeled rat liver ribosomal ribonucleic acids with snake venom phosphodiesterase.


Inorganic pyrophosphate as an energy donor in photosynthetic and respiratory electron transport phosphorylation systems.


The effect of UV-irradiation on the plaque-forming ability of single- and double-stranded polyoma virus DNA.


IUPAC-IUB Commission on Biochemical Nomenclature. Rules for naming synthetic modifications of natural peptides. Tentative rules.


The specificities and configurations of ternary complexes of yeast and liver alcohol dehydrogenases. 1. Some aspects of the substrate specificities of liver and yeast alcohol dehydrogenases have been investigated with pentan-3-ol, heptan-4-ol, (+)-butan-2-ol, (+/-)-butan-2-ol, (+/-)-hexan-3-ol and (+/-)-octan-2-ol as potential substrates. The liver enzyme is active with all substrates tested, including both isomers of each optically active alcohol. In contrast, the yeast enzyme is completely inactive towards those secondary alcohols where both alkyl groups are larger than methyl and active with only the (+)-isomers of butan-2-ol and octan-2-ol. 2. The absence of stereospecificity of liver alcohol dehydrogenase towards optically active secondary alcohols and its broad specificity towards secondary alcohols in general are explained in terms of an alkyl-binding site that will react with a variety of alkyl groups and the ability of the enzyme to accommodate a fairly large unbound alkyl group in an active enzyme-NAD(+)-secondary alcohol ternary complex. The absolute optical specificity of the yeast enzyme towards n-alkylmethyl carbinols and its unreactivity towards pentan-3-ol, hexan-3-ol and heptan-4-ol are explained by its inability to accept alkyl groups larger than methyl in the unbound position in a viable ternary complex. 3. Comparison of the known configurations of the n-alkylmethyl carbinols and [1-(2)H]ethanol and [1-(3)H]geraniol, which have been used in stereospecificity studies with these enzymes by other workers, provides strong evidence for which alkyl group of the substrate is bound to the enzyme in the oxidation of n-alkylmethyl carbinols. The conclusions reached are, for butan-2-ol oxidation with the liver enzyme, confirmed by deductions from kinetic data obtained with (+)-butan-2-ol and a sample of butan-2-ol containing 66% of (-)-butan-2-ol. 4. Initial-rate parameters for the oxidations of (+)-butan-2-ol, 66% (-)-butan-2-ol and pentan-3-ol by NAD with liver alcohol dehydrogenase are presented. The data are completely consistent with a general mechanism of catalysis previously proposed for this enzyme.


The metabolism of the isomeric tert.-butylcyclohexanones. 1. (+/-)-2-, (+/-)-3- and 4-tert.-Butylcyclohexanone are reduced in the rabbit to secondary alcohols, which are excreted extensively conjugated with glucuronic acid. 2. The major metabolite of (+/-)-2-tert.-butylcyclohexanone is (+)-cis-2-tert.-butylcyclohexanol, which has been isolated from the urine as [(+)-cis-2-tert.-butylcyclohexyl beta-d-glucosid]uronic acid. The minor metabolite is (+)-trans-2-tert.-butylcyclohexanol. 3. (+/-)-3-tert.-Butylcyclohexanone is reduced mainly to (+/-)-cis-3-tert.-butylcyclohexanol, and to a smaller extent to (+/-)-trans-3-tert.-butylcyclohexanol. 4. 4-tert.-Butylcyclohexanone yields mainly the trans-alcohol, which is excreted in conjugated form and has been recovered from the urine as (trans-4-tert.-butylcyclohexyl beta-d-glucosid)uronic acid. The cis-alcohol is formed to a minor extent and excreted in conjugated form. 5. The ratios of the amounts of cis- to trans-alcohols produced by the three ketones differed from the relative amounts of cis- and trans-alcohols produced by the corresponding methylcyclohexanones. 6. From these findings the suggestion is made that two orientations of ketone relative to coenzyme occur: alcohols with an equatorially orientated hydroxyl group are thought to be produced as a result of a ;face-to-face' interaction with NADH, and alcohols with axially orientated hydroxyl groups as a result of a ;perpendicular' interaction. Which will predominate is thought to depend on steric factors, particularly the size and position of alkyl substituents in the substrate.


Studies on brain-cortex slices. Differences in the oxidation of 14-C-labelled glucose and pyruvate revealed by the action of triethyltin and other toxic agents. 1. The rate of appearance of (14)CO(2) from [6-(14)C]glucose and [3-(14)C]pyruvate was measured. Pyruvate is oxidized to carbon dioxide twice as fast as glucose, although the oxygen uptake is almost the same with each substrate. 2. The presence of 30mum-2,4-dinitrophenol increases the output of (14)CO(2) from [6-(14)C]glucose sixfold whereas the oxygen uptake is not quite doubled. Similar results are obtained with 0.1m-potassium chloride. The stimulating action of these two agents on the output of (14)CO(2) from [3-(14)C]pyruvate is much less than on that from [6-(14)C]glucose. 3. The effects of oligomycin, ouabain and triethyltin on the respiration of control and stimulated brain-cortex slices were studied. Triethyltin (1.3mum) inhibited the oxidation of [6-(14)C]glucose more than 70%, but did not inhibit the oxidation of[3-(14)C]pyruvate. [3-(14)C]pyruvate. 4. The production of lactic acid by brain-cortex slices incubated with glucose is twice as great as that with pyruvate. Lactic acid increases two and a half times in the presence of either triethyltin or oligomycin when the substrate is glucose, but is no different from the control when the substrate is pyruvate. 5. With kidney slices the production of lactic acid from glucose is very low. It is increased by oligomycin but not by triethyltin. 6. The results are discussed in terms of the oxidation of the extramitochondrial NADH(2) produced during glycolysis.


The synthesis of polydeoxyribonucleotide by cell-free extracts of embryonic mouse tissue. The conditions under which soluble extracts prepared from mouse embryos incorporate [(3)H]thymidine 5'-triphosphate into polydeoxyribonucleotide have been studied. In common with similar preparations from other mammalian tissues, mouse-embryo DNA nucleotidyltransferase requires the four complementary deoxyribonucleoside 5'-triphosphates, primer DNA and a bivalent cation for activity. Unlike other mammalian DNA nucleotidyltransferases, the rate and extent of the incorporation of [(3)H]thymidine 5'-triphosphate is much greater with Mn(2+) than with Mg(2+) and, with either Mg(2+) or Mn(2+), maximum activity occurs at pH6.4. The difference between Mg(2+) and Mn(2+) varies markedly with pH, reaching a maximum of six- to eight-fold at pH6.4.


Isolation of deoxyribonucleic acid from mammalian tissues. 1. DNA has been isolated from different mammalian tissues. The DNA preparations were free from RNA, protein and polysaccharides and have a similar range of sedimentation coefficients (approx. 24s). 2. Protein was removed by a two-stage extraction with a phenol-cresol mixture by using a detergent with 4-aminosalicylate in the first stage and sodium chloride in the second. 3. Polysaccharides remained in solution when DNA was precipitated with 2-butoxyethanol in the presence of 0.5m-sodium chloride and 1.5m-sodium benzoate. 4. Ribosomal RNA was removed by precipitation in the presence of 3m-sodium chloride at 0 degrees , when DNA remained soluble.


A simple procedure for the estimation of very small amounts of nitrogen in lipids. 1. About 0.1mug.atom of combined nitrogen, in lipids and a few other compounds, can be determined quantitatively by the gentle digestion of dry samples in 10ml. test tubes with perchloric acid for 30min., followed by the estimation of the resulting ammonia as the stable blue colour (lambda(max.) 635mmu) produced by the addition of phenol and nitroprusside, an alkaline phosphate buffer and alkaline hypochlorite. 2. Deionized distilled water is required, but the other reagents need no special purification if chosen and handled with care. 3. The results are linear with from 0.015 to 0.15mug.atom of nitrogen, or up to 1mug.atom if the final solutions are diluted with water after full colour development.


Control of glycolysis and gluconeogenesis in rat kidney cortex slices. 1. Glucose uptake or glucose formation has been studied in kidney cortex slices to investigate metabolic control of phosphofructokinase and fructose-diphosphatase activities. 2. Glucose uptake is increased and glucose formation is decreased by anoxia, cyanide or an uncoupling agent. Under these conditions the intracellular concentrations of glucose 6-phosphate and ATP decreased whereas that of fructose diphosphate either increased or remained constant, and the concentrations of AMP and ADP increased. 3. Glucose uptake was decreased, and glucose formation from glycerol or dihydroxyacetone was increased, by the presence of ketone bodies or fatty acids, or after starvation of the donor animal. Under these conditions, the concentrations of glucose 6-phosphate and citrate were increased, whereas those of fructose diphosphate and the adenine nucleotides were unchanged (see also Newsholme & Underwood, 1966). 4. It is concluded that anoxia and cell poisons increase glucose uptake and decrease gluconeogenesis by stimulating phosphofructokinase and inhibiting fructose diphosphatase, whereas ketone bodies, fatty acids or starvation increase gluconeogenesis and decrease glucose uptake through the citrate inhibition of phosphofructokinase.


The stereochemistry of hexahydroprenol, ubiquinone and ergosterol biosynthesis in the mycelium of Aspergillus fumigatus Fresenius. 1. The mycelium of Aspergillus fumigatus has been shown to incorporate mevalonate into squalene, ubiquinone, ergosterol and hexahydroprenol. 2. The (3)H/(14)C ratio in ubiquinone, biosynthesized from [2-(14)C-(4R)-4-(3)H(1)]mevalonate, is the same as in the squalene; essentially no (3)H was incorporated from [2-(14)C-(4S)-4-(3)H(1)]mevalonate, indicating the biosynthesis of biogenetically trans-isoprene units. 3. The (3)H/(14)C ratio for ergosterol (from ;4R-mevalonate') was 3:5, showing that the proton at C-24 is not lost during alkylation of the side chain; it probably migrates to C-25. 4. As (3)H from both mevalonates was incorporated into the hexahydroprenols the biosynthesis of both cis- and trans-isoprene units must occur. 5. The saturated omega- and psi-isoprene units are shown to be biogenetically trans, as are two of the unsaturated residues. 6. The saturated alpha- and unsaturated beta-isoprene residues are both biogenetically cis. 7. An inexplicable loss of approximately half of the olefinic protons from the cis-portion of hexahydroprenol occurs; possible reasons for this loss are discussed. 8. Increase in chain length of the hexahydroprenols is by a cis addition. 9. A biosynthesis of hexahydroprenols by addition of cis-isoprene units to all-trans-geranylgeranyl pyrophosphate, or a dihydro or tetrahydro derivative thereof, is suggested.


A note on the effect of different pH values on polyoma virus.


The purification of 3,3-dimethylallyl- and geranyl-transferase and of isopentenyl pyrophosphate isomerase from pig liver. The enzyme catalysing the synthesis of farnesyl pyrophosphate from dimethylallyl pyrophosphate and isopentenyl pyrophosphate, or from geranyl pyrophosphate and isopentenyl pyrophosphate, has been purified 100-fold from homogenates of pig liver. The enzyme has optimum pH 7.9 and requires Mg(2+) as activator in preference to Mn(2+); it is inhibited by iodoacetamide, N-ethylmaleimide, p-hydroxymercuribenzoate and phosphate ions in addition to the products of the reaction, inorganic pyrophosphate and farnesyl pyrophosphate. From product-inhibition studies of the geranyltransferase reaction, the order of addition of substrates to and release of products from the enzyme has been deduced: geranyl pyrophosphate combines with the enzyme first, followed by isopentenyl pyrophosphate. Farnesyl pyrophosphate dissociates from the enzyme before inorganic pyrophosphate. The existence of isopentenyl pyrophosphate isomerase in liver is confirmed. Methods for the preparation of the pyrophosphate esters of isopentenol, 3,3-dimethylallyl alcohol, geraniol and farnesol are also described.


Glucose-6-phosphatase assay on microgram amounts of liver tissue.


Clinical applicability of urea-LDH test in various pyruvate concentrations.


Myelography in dogs, using water-soluble contrast mediums.


Studies on the human oocyte and its follicle. I. Ultrastructural and histochemical observations on the primordial follicle stage. Oocytes in primordial ("resting") follicles in adult human ovaries contain a complex paranuclear structure identified by light microscopists as Balbiani's vitelline body. By electron microscopy this structure is composed of a mass of mitochondria with associated endoplasmic reticulum, multiple compound aggregates which form a ring around the cytocentrum, and a single stack or coil of annulate lamellae either attached to the nuclear membrane or free in the cytoplasm. The compound aggregates contain vacuoles and finely divided electron-opaque material. Evidence is presented for the probable transport of this material between the oocyte and its environment. The cytocentrum contains a central aggregate of amorphous electron-opaque deposits which appear to become periodically aligned on fine fibrils to form the long coarse fibers at the periphery of the cytocentrum. The apparent prevalence of annulate lamellae attached or adjacent to the nuclear membrane of oocytes in ovaries removed during the mid-follicular (estrogenic) phase of the cycle indicates the need for further study of a possible hormonal influence on the resting oocyte. By light microscopy phosphatases were not found within the oocyte, but adenosine-monophosphatase activity is present in the cortical cells surrounding primordial follicles, and also at the periphery of each primitive follicle cell, most prominently at the oocyte side. Glucose-6-phosphate dehydrogenase activity is present within the oocyte cytoplasm.


Androgen synthesis in a patient with virilizing adrenocortical carcinoma.


The effects of acetaldehyde on serotonin metabolism.


Neurologic symptoms in lymphoma. I. Hodgkin's disease.


Electronic effects in horse liver alcohol dehydrogenase catalysis.


The role of essential fatty acids in adrenocortical function.A collective review.


Action of N'N'-anhydro-bis (beta-hydroxyethyl) biguanide hydrochloride (ABOB) on reproduction of Sendai virus (Myxovirus parainfluenzae 1) in chick embryos.


Prednisolone-psychosis.

Prednisolone-psychosis.


The effect of conjugated estrogens and estriol succinate on fibrinolysis. A clinical correlation in patients with uterine bleeding.


An attempt to treat myocarditis induced in dogs after a modified method of Cavelti.


The enzymatic degradation of ultraviolet-irradiated deoxyribonucleic acid.


Apparent ESR photosignals in melanin.

Apparent ESR photosignals in melanin.


Solid benign tumors of the esophagus.

Solid benign tumors of the esophagus.


Studies of avian sarcoma and erythroblastosis (strain 13). VI. Response in birds to two routes of induction and pathology of a virus-induced tumor.


Experimental pox infection in waterfowl.


Isolation of a phospholipid renin inhibitor from kidney.


Spectroscopic studies on spinach ferredoxin and adrenodoxin.


Purification and properties of two acid phosphatase fractions isolated from osmotic shock fluid of Escherichia coli.


Glucokinase from Entamoeba histolytica and related organisms.


Nucleoside phosphonic acids. II. The synthesis of 5'-deoxythymidine 5'-phosphonic acid and its pyrophosphate derivatives.


Effect of graded doses of ACTH on parotid fluid corticosteroid levels.


Action of triton X-100 on ultrastructure and membrane-bound- enzymes of isolated nerve endings from rat brain.


Nutritional and genetic influences on the dentition of "ia" and "pia" rats.


Free radicals of biological interest. I. Electron spin resonance spectra of tobacco smoke condensates.


Reduced nicotinamide-adenine dinucleotide oxidation in Escherichia coli particles. 3. Cellular location of menadione reductase and ATPase activities.


Effects of heparin in vivo on lysosomal enzymes in rat plasma.


Comparative effects of aldosterone and heat acclimatization on oxidative enzymes in rat tissues.


The incorporation of palmitate-1-14C by rat lung in vitro.


The simultaneous explantation of the nerve and sarcomatous tissue.


Abnormalities of skeletal muscle maturation in brain damaged children: a histochemical study.


Virulence of variola virus isolated from smallpox cases of varying severity.


Pituitary hormones and the metabolism of N-hydroxy-N-2-fluorenylacetamide in female rats.


The patient with recurrent aphthous ulcers and the patient with recurrent herpes labialis: a study of two population samples.


Antigenic identity of interferons induced by different viruses in the same cell system.


Adenovirus infection of human embryo liver cells.


Eosinophilic response in glioblastoma tissue culture after addition of autologous lymphocytes. In experiments designed for the study of interactions in vitro between the cells of glioblastoma multiforme and lymphocytes from the same donor, a marked eosinophilic response was noted in three out of six cases. This was an entirely unexpected finding since few, if any, eosinophils survive in tissue cultures. These cells incorporated (3)H-cytidine and (3)H-leucine 72 hours after the cultures had been established, an indication that they were metabolically active.


Effects of renin and ACTH on adrenal steroid secretion in the American bullfrog.


Sympathetic and humoral vasoconstrictor responses in deoxycorticosterone hypertension.


Interferon production with strain SF-4 of parainfluenza-3 virus.


Plaque characteristics of four classes of bovine viruses.


Susceptibility of tissue culture cells to foot-and-mouth disease virus: concentration of bovine serum in culture medium.


Rates of hydrocortisone and corticosterone secretion in calves.


Isolation and characterization of bovine enteric viruses.


Portal vein thromboses in malignant hepatoma.


Impairment of pituitary adrenocorticotrophic function by corticosterone in the blood.


Acute necrotizing encephalitis.

Acute necrotizing encephalitis.


Study on glucose-6-phosphatase activity in human serum.


Metabolism of aromatic amino acids in collagen diseases: urinary excretion of 3-methoxy-4-hydroxymandelic acid and the interference of 3-methoxy-4-hydroxyphenylpyruvate.


Biochemical correlates of respiratory deficiency. 8. A precipitating antiserum against cytochrome oxidase of yeast and its use in the study of respiratory deficiency.


Virus study of pock disease among buffaloes.


Perforation of peptic ulcer during early management of myocardial infarction.


Radioautographic localization of the increased synthesis of phosphatidylinositol in response to pancreozymin or acetylcholine in guinea pig pancreas slices. A technique is described for measuring the incorporation of myo-inositol-2-(3)H into the lipid of various regions of the guinea pig pancreatic acinar cell by radioautography. Stimulation of enzyme secretion with either pancreozymin or acetylcholine was associated with increased graining in both the basophilic cytoplasm and the nonbasophilic cytoplasm. Kinetic studies suggested that the incorporation of myo-inositol-2-(3)H was stimulated independently in the two regions. Most of the increment in graining due to stimulation with pancreozymin or acetylcholine plus eserine was abolished if the tissue was extracted with 2:1 chloroform-methanol before radioautography. On chromatography of lipid extracts of pancreas, the only lipid showing a detectable increment in radioactivity on stimulation with pancreozymin was phosphatidylinositol. Thus, essentially all of the increment in graining is likely to be due to increased incorporation of tritium into phosphatidylinositol. These studies, coupled with earlier studies employing differential centrifugation, indicate that on stimulation of enzyme secretion there is increased synthesis of phosphatidylinositol in the rough-surfaced endoplasmic reticulum and in the smooth-surfaced Golgi membranes. The significance of these observations is discussed in connection with membrane circulation presumed to occur in the pancreatic acinar cell on stimulation of protein secretion. It is suggested that the increased synthesis of phosphatidylinositol may be concerned with the formation of new endoplasmic reticulum and possibly Golgi membrane to replace that which is presumably converted to membrane of the zymogen granules during intracellular protein transport.


An in vitro effect of triiodothyronine on rat adipose tissue. Triiodo-L-thyronine (T(3)) added in vitro to fat pads from normal, or propylthiouracil-treated rats enhanced the rate of release of glycerol and free fatty acids (FFA) in the presence of epinephrine. An effect of T(3) was also demonstrated in the presence of adrenocorticotropic hormone (ACTH), thyroid-stimulating hormone, or glucagon in studies with tissue from normal rats. The minimal effective concentration of T(3) was approximately 2.5 x 10(-5) mole/liter for intact fat pads and 3 x 10(-6) mole/liter for fat cells. With fat pads from propylthiouracil-treated rats the effect of T(3) was not apparent until the 3rd hr of incubation. Enhancement of epinephrine-stimulated lipolysis by T(3) was evident during the 1st hr of incubation of fat pads from normal rats, and fat cells responded almost immediately to the presence of T(3). When added alone or in the presence of theophylline, 3',5'-adenosine monophosphate or its dibutyryl derivative, T(3) had little or no effect on lipolysis. The effect of T(3) was observed with or without glucose in the medium, and was not inhibited by cycloheximide or actinomycin D. It did not persist when tissues, after incubation in the presence of T(3) were transferred to medium without T(3). No effect of T(3) on glucose uptake in the presence of epinephrine, ACTH, or insulin was demonstrated.


Experimental viral hepatitis in the dog: production of persistent disease in partially immune animals. Experimental infection with canine hepatitis virus has been studied in a series of 49 dogs. The pattern of response to infection was distinctly modified by the immune status of the animal. All of 19 fully susceptible dogs had an acute, fulminating fatal hepatitis when infected with a standard dose of virus, and all of 19 dogs with high levels of immunity to the virus survived without apparent illness. However, 11 dogs were spontaneously encountered with partial immunity to the infectious agent, and these animals developed different, prolonged forms of hepatitis following infection. In four animals death occurred in 8-21 days following what may be called a subacute course. The remaining seven dogs survived up to 8 months with evidence of chronic hepatic damage. The subacute and chronic forms of hepatitis were reproduced experimentally in seven of eight fully susceptible dogs which were passively immunized against the canine hepatitis virus by administration of hyperimmune serum. Although the virus could be found in sites of hepatic damage in the early stages of the subacute and chronic diseases, it could not be demonstrated in the later stages which were characterized by persistent hepatic damage and a marked chronic inflammatory reaction. Dogs with chronic hepatitis eventually developed extensive hepatic fibrosis. The pathologic, physiologic, virologic, and immunologic features of these experimental forms of viral hepatitis are described.


Spin-label determination of enzyme symmetry.


High-resolution electron spin resonance studies of hyperfine inter actions in substituted 1,4-naphthoquinones and naphthazarins.


Electron paramagnetic resonance study of L-alanine irradiated at low temperatures.


Trophoblastic disease.

Trophoblastic disease.


Activation of production of infectious tumor virus SV40 in heterokaryon cultures.


Spin-label study of hemoglobin conformations in solution.


Carbohydrate transport in Staphylococcus aureus. V. The accumulation of phosphorylated carbohydrate derivatives, and evidence for a new enzyme-splitting lactose phosphate.


Japanese B encephalitis. Symptom dynamics in an organic psychosis.


Regulation of fructose diphosphatase activity by disulfide exchange.


Studies on infectious laryngotracheitis (ILT) virus.


Some observations on herpes virus mammillitis of bovine animals.


Presence of type C virus particles and their aberrant form in mammary carcinoma of a strain C58 mouse.


The properties of cell clones derived from a polyoma induced mouse tumor.


Sporulation of Clostridium perfringens (welchii) in four laboratory media.


On the heredity of ESR spectra intensity of the human hair.


Structure and function of the mitochondria with a brief note on blood cell mitochondria.


The effects of neuromuscular blocking agents on mitochondria. 3. Effects of D-tubocurarine and pyrrolizidine alkaloids on the uptake and depletion of cations.


Electron spin resonance study on the photoinduced radicals from carcinogenic 4-nitroquinoline 1-oxide.


Clinical effects of vinblastine on chorionic tumors.


Angiofibroma of the ethmoidal sinus in a female.


Zeta potential in the development of pharmaceutical suspensions.


Antigenic response in cattle to Clostridium welchii type D vaccine.


Clostridal infection of the uterus. A review treatment with hyperbaric oxygen.


Renin substrate in plasma under various experimental conditions in the rat.


The vitamin K content of beef liver. Detection of a new form of vitamin K.


Vitamin D stimulation of [3H]orotic acid incorporation into ribonucleic acid of rat intestinal mucosa.


Diphosphopyridine nucleotide specific isocitric dehydrogenase of mammalian mitochondria. I. On the roles of pyridine nucleotide transhydrogenase and the isocitric dehydrogenases in the respiration of mitochondria of normal and neoplastic tissues.


Studies on the deproteinization of DNA.

Studies on the deproteinization of DNA.


Synthesis of a polydeoxyribonucleotide containing an internal pyrophosphate linkage.


Organic fluorine compounds. 3. Action of perchloryl fluoride on substituted ethyl cyanoacetates and animal toxicities of the fluorinated products.


Methods of storing viruses at low temperatures with particular reference to the Myxovirus group.


Mitochondrial polymorphism in maize. I. Putative evidence for de novo origin of hybrid-specific mitochondria.


Studies on the locus of the enzyme recognition site in phenylalanine transfer RNA.


On the production of highly labeled tumor virus deoxyribonucleic acid.


The presence of adenovirus-specific antigens in hamster cells rendered neoplastic by adenovirus 1-SV40 and adenovirus 2-SV40 hybrid viruses.


The value of the electromyogram in the diagnosis of pressure neuropathy from "hanging arm".


Neuropathy following irradiation. An unusual late complication of radiotherapy.


Technetium-iron-ascorbic acid complex. A good brain scanning agent.


Flavoproteins of the mitochondrial respiratory chain.


The cytochrome system of Bacillus megaterium KM. The presence and some properties of two CO-binding cytochromes.


Oxidative phosphorylation in nuclei isolated from rat thymus.


Reconversion of detergent- and sulfhydryl reagent-produced P-420 to P-450 by polyols and glutathione.


The chemistry of flavins and flavoproteins. 3. The reaction of dihydrolipoic acid with flavins.


On the possible role of structural protein in the binding and translocation of adenine nucleotides in mitochondria.


Target size of components in oxidative phosphorylation. Studies with a linear accelerator.


The effect of light on flavoproteins in the presence of alpha-keto acids.


Value of the ninhydrin test in investigation of tactile sensitivity.


Electron spin resonance of cytochrome b2 and of cytochrome b2 core.


ATPase activities of rat epididymal adipose tissue.


Oxygen evolution by isolated chloroplasts with carbon dioxide as the hydrogen acceptor. A requirement for orthophosphate or pyrophosphate.


Widespread necrotizing arteritis in rheumatoid arthritis: report of a patient who survived.


On the central hypertensive effect of angiotensin II.


Interactions of substrates, inhibitors, and coenzymes at the active site of horse liver alcohol dehydrogenase.


Biosynthesis of urea. XII. Further studies on argininosuccinate synthetase: substrate affinity and mechanism of action.


Electromagnetic properties of hemoproteins. II. The effect of  physical states on electron paramagnetic resonance parameters of hemoproteins.


Electromagnetic properties of hemoproteins. I. Electron paramagnetic resonance absorptions of single crystals of ferrimyoglobin and cytochrome c peroxidase.


Histochemistry of hidradenoma and eccrine spiradenoma.


Hepatic lobectomy for teratoid liver cancer. Report of a seven-year cure.


Intravascular coagulation and fibrinolysis in septic abortion.


Phenotypic expression of transformation: induction in cell culture by a phorbol ester. A biologically potent, tumor-promoting phorbol ester from Croton tiglium L. induces the appearance of transformed clones in a population of contact-inhibited fibroblasts of the mouse cell line 3T3. Mixed populations of cells with 10,000 3T3 cells and 100 virus-transformed cells were exposed to the phorbol ester and, in comparison with untreated cells, showed a marked increase in the numbers of transformed clones that grew. Exposure of 3T3 cultures to the carcinogen 7,12-dimethylbenz(a)anthracene alone or prior to exposure to the phorbol ester did not cause any increase in the number of transformed clones.


Epithelial-mesenchymal interactions.

Epithelial-mesenchymal interactions.


Roentgen diagnosis in lymphedema.

Roentgen diagnosis in lymphedema.


Experimental studies on continuous intra-arterial infusion of anti-tumor agents (with special emphasis on histochemical changes).


The ultrastructure of a human glioblastoma multiforme-derived tumor heterologously transplanted to guinea pig eye and brain.


Biochemical studies of otosclerosis: enzymes and protein in malleus and incus.


Increased incidence of hepatoma in mice with chronic schistosomiasis mansoni treated with a carcinogen.


Electron microscopy of a benign epidermal pox disease of rhesus monkeys.


The removal of non-specific inhibitors from human and animal sera to mumps and para-influenza type I viruses by kaolin.


Single-strand scissions induced in circular and linear lambda DNA by the presence of dithiothreitol and other reducing agents.


Collagen synthesis on polyribosomes of cultured mammalian fibroblasts.


D-Tyrosyl RNA: formation, hydrolysis and utilization for protein synthesis.


Physico-chemical studies of three variants of Mengo virus and their constituent ribonucleates.


The progressive pressor response to angiotensin in the rabbit. 1. The threshold for any detectable rise of systemic arterial pressure during the prolonged intravenous administration of angiotensin to conscious rabbits was observed to be an infusion rate of 0.003-0.006 mug.kg(-1).min(-1).2. At infusion rates between threshold and 0.04 mug.kg(-1).min(-1) the systemic arterial pressure rose progressively over a 3- to 7-day period to a plateau.3. On stopping the angiotensin infusion the blood pressure fell rapidly back to its base line much faster than it rose during the infusion. The time taken to reach control values was approximately related to the duration of the infusion.4. At infusion rates of about 0.05 mug.kg(-1).min(-1) the full rise of blood pressure developed within a few minutes, and could be sustained without change for many days. At higher rates the blood pressure diminished with time.5. Diurnal fluctuations of blood pressure were often seen during prolonged infusions of angiotensin at low rates; and more rapid fluctuations of blood pressure over an hour or two were frequently encountered immediately after an infusion was turned off.6. The possible role of angiotensin in producing chronic renal hypertension is discussed in the light of these observations.


Acute renal failure caused by hypersensitivity to polymyxin B sulfate.


Electrocardiographic abnormalities and some myocardial enzymatic disturbances in experimental poisoning with metallic mercury.


Comparison of adrenocortical reserves with long-acting  corticotropin preparations in the two-day intramuscular corticotropin test in man.


Fluorimetric determination of corticosteroids in human plasma.


Chemical bonding information from photoelectron spectroscopy. High-resolution measurements of photoelectrons produced by x-rays in compounds of iodine and europium have revealed chemical shifts in the core-level energies, from which chemical bonding information can be obtained. The observed shifts, 0.8 electron volt per unit change in oxidation number in iodine and 9.6 electron volts in europium, are discussed in terms of two theoretical models.


Cellular immunity factors in infection with polyoma virus. I. Levels of inhibitors of the polyoma virus and interferon-like substances in organs and tumors in hamsters infected with polyoma virus.


Studies on the etiology of viral hepatitis (VH). I. Isolation attempts of the etiologic agent by various methods.


Studies on the etiology of viral hepatitis. II. Media stimulating growth of viruses.


Goltz's syndrome: focal dermal hypoplasia. A combined mesoectodermal dysplasia.


A comparison of normal red cell ATP levels as measured by the firefly system and the hexokinase system.


Treatment of peripheral nerve injuries.

Treatment of peripheral nerve injuries.


Myxovirus influenza A isolated from ducklings. A strain of haemagglutinating virus isolated by Walker and Bannister in 1953 and identified by them as "Filterable Agent" of ducks has been examined. The evidence suggests that although belonging to the Myxovirus Influenza Type A group, it possesses specific strain properties.


Selective heat sensitivity of cancer cells. Biochemical and clinical studies.


Effectiveness of irradiation therapy in peripheral neuropathy caused by malignant disease.


"Mesenchymal" chondrosarcoma, a rare malignant chondroid tumor usually primary in bone. Report of a case arising in extraskeletal soft tissue.


Prediction of adult stature.

Prediction of adult stature.


Radioimmunoassays. II. An evaluation of the use of radioimmunoassay for the measurement of protein hormones.


Functional adrenocortical carcinoma in a dog.


Studies on the chemistry of hemoglobin. II. The effect of salts on the dissociation of hemoglobin into subunits.


The purification and properties of a thyroid-stimulating factor isolated from Clostridium perfringens.


Biosynthesis of retinoic acid by intestinal enzymes of the rat. The incubation of beta-carotene-(14)C with the soluble fraction of the intestinal mucosa resulted in the formation of small amounts of acidic material. The addition of NAD or NADH to the soluble fraction caused a tenfold increase in this material. Incubation of retinal-15-(14)C with the soluble fraction of the intestinal mucosa plus NAD or NADH resulted in the conversion of 80-90% of the retinal to acidic material, which has been shown to contain retinoic acid. In vivo studies on the formation of retinoic acid in the intestinal mucosa after the administration of beta-carotene-(14)C revealed that an appreciable amount of beta-carotene was converted to acidic compounds. When retinal-15-(14)C was administered, portal blood contained 30-40% of the absorbed radioactivity. 24% of this radioactivity was found in acidic material, which has been shown to contain retinoic acid. It is suggested that enzymes in rat intestine cleave beta-carotene to retinal and oxidize the latter to retinoic acid, which is then transported via the portal circulation to the liver.


Lipid composition of mitochondria from bovine heart, liver, and kidney. Highly purified preparations of mitochondria from bovine heart, liver, and kidney were isolated and characterized by electron microscopy, oxidative phosphorylation ability, cytochrome c reductase activity, and cytochrome content. Components of lipid extracts of the preparations were determined by thin-layer chromatography, diethylaminoethyl-cellulose column chromatography, and spectrophotometric procedures. The major phospholipids were identified by their chromatographic behavior, IR spectrometry, and paper chromatography of their hydrolysis products. The lipid content of the mitochondria paralleled that of the components of the electron transfer chain, heart mitochondria being richest and liver mitochondria poorest in lipid. Heart mitochondria contain equal concentrations of coenzyme Q and cholesterol (1%); the highest cholesterol content (4.7%) was found in mitochondria from kidney. The phospholipids of mitochondria from the three organs were qualitatively and quantitatively very similar. The major polar lipid components (cardiolipin, choline glycerophosphatides, and ethanolamine glycerophosphatides) were present in a molar ratio of 1:4:4. It is suggested that mitochondria from different sources contain characteristic lipids, mainly phospholipids, of which cardiolipin is particularly diagnostic of the source of the mitochondria.


Removal of the sphingolipid impurity from preparations of yeast phosphatidyl inositol. A complex sphingolipid containing inositol and mannose, present in lipid extracted from toluene-autolyzed baker's yeast, was eluted from silicic acid columns immediately after phosphatidyl inositol, and was the main nitrogenous impurity in crude preparations of this phospholipid. Nitrogenfree phosphatidyl inositol was obtained by rechromatography on alumina. Modifications to the chromatographic procedure also gave diphosphatidyl glycerol containing the theoretical 4.29% P.


Complement fixation test of equine infectious anemia. II. Relationship between CF antibody response and the disease.


Complement fixation test of equine infectious anemia. I. Specificity of the test.


Specific complement fixation test of Rous sarcoma with pigeon serum.


Mixed infection with avian adenovirus and fowl pox virus.


Adrenocorticotrophic activity in plasma of patients with acromegaly.


Plasma renin activity in hypertension.

Plasma renin activity in hypertension.


Dissecting perforation of infarcted interventricular septum with associated posterior papillary muscle involvement.


Value of perlingual lymphography in cancer of the head and neck. A means of exploration of the lymphatic system of the neck.


Bronchography during infancy and childhood.


Subdiaphragmatic infection.

Subdiaphragmatic infection.


Clinical and therapeutic aspects of schistosomiasis.


Oxidoreductive and hydrolytic enzyme patterns in plant suspension culture cells. Local and time relationships.


Dissociation between inhibition of movement and inhibition of division in RSV transformed human fibroblasts.


Aminoglutethimide (Elipten-Ciba) as an inhibitor of adrenal steroidogenesis: mechanism of action and therapeutic trial.


Circulating viral and thyroid antibodies in subacute thyroiditis.


Cushing's syndrome in association with a carcinoid tumor of the lung.


Effect of estrogen therapy on carbohydrate metabolism in acromegaly.


Failure of metyrapone to inhibit 11-hydroxylation of 11-deoxycortisol during drug therapy.


Gastric mucosal injury by anti-inflammatory drugs.


Hormone-secreting tumours of the pancreas.


Electron spin resosnance of gamma-irradiated oriented DNA prepared by wet spinning. When moist, oriented DNA is gamma-irradiated and its electron spin resonance spectrum is recorded at 77 degrees K, an unresolved spectrum is obtained, the amplitude of which is strongly dependent on the angle between the direction of the DNA helices and the field. Annealing at 199 degrees K gives an eight-line thymine-like spectrum which also has a marked angular dependence. For dry, oriented DNA, the unresolved spectrum dominates even at room temperature, and the spectra exhibit lower degrees of anisotropy.


Tissue specificity of histone phosphorylation. The incorporation of (32)P-phosphate into histone fractions isolated from normal, hepatectomized, and Novikoff hepatoma-bearing rats was investigated. Varying degrees of phosphorylation were exhibited by different histone fractions. The phosphorylation of histones is tissue specific and appears to be correlated with metabolic cell functions, that is, it decreases with increasing mitotic activity of the cells.


Adenovirus infections in Indian army personnel.


Investigation of treatment for jaundice in newborn infants.


The mechanism of contracting action of angiotensin on the isolated aortic strip of rabbit.


An estrogen-producing "yellow tumour" of the ovary associated with arterial hypertension. (A case report contributing to the problem of classification of functioning ovarian neoplasms).


Pharmacology and diagnosis of pesticide-induced illnesses. Although emphasis upon the concept of environmental health is a recent development in the medical community at large, the environment in which man worked has been of considerable concern to physicians in occupational medicine for a quarter of a century or more. Among the environmental factors which may adversely affect the health of exposed persons are the pesticides. The pharmacologic properties of these chemicals as well as the diagnostic signs and symptoms are presented herein.


Cardiac accelerator mechanisms of angiotensin.


Effect of carzinophilin on the metabolism of nicotinamide adenine dinucleotide in the Ehrlich ascites cells in vitro.


Demonstration of Entamoeba histolytica from liver biopsy specimen.


Juvenile nasopharyngeal angiofibroma. (Report of a case with unusual features).


Cell transformation of established human cell line induced by SV-40 DNA.


Chemotherapy of glioblastoma multiforme: an appraisal.


Studies in the reliability of assessing skeletal maturity from x-rays. 3. Greulich-Pyle Atlas and Tanner-Whitehouse method contrasted.


Salicylic acid in the treatment of diabetic, neurotrophic and ischemic ulcers.


Effect of progressive starvation on rat liver enzyme activities.


Aspects of liver lipid metabolism in the biotin-deficient rat.


Simultaneous determination of renin activity and angiotensin concentration levels in human plasma.


Effect of ACTH and prednisolone on Japanese encephalitis.


Respiratory syncytial virus infections in families. A study of family members of children hospitalized for acute respiratory disease.


Myelin artifacts.

Myelin artifacts.


Homeostatic role of the renal-adrenal axis in bacteremic shock. Its therapeutic implications.


Studies of a biochemical lesion in experimental tuberculosis in mice. V. Further study on a toxic lipid fraction in firmly bound lipids.


Eosinopenic response of asthmatic children to ACTH.


The place of corticosteroids in pulmonary disease.


Multiple granular cell myoblastomas in children.


Autoimmune progesterone urticaria.

Autoimmune progesterone urticaria.


Virus transfer through a sewage disposal unit.


Lethal toxin of Bacillus cereus. I. Relationships and nature of toxin, hemolysin, and phospholipase. Bacillus cereus phospholipase was characterized as a phospholipase C by the analysis of lecithin degradation products by thin-layer and paper chromatography. Methanol in the growth menstruum inhibited completely the synthesis of phospholipase C, whereas the synthesis of lethal toxin and hemolysin were only partially inhibited. Dialysis of preformed B. cereus products against ethyl alcohol and methanol did not inactivate hemolytic, phospholipase C, or lethal activity. The hemolytic and lethal activities of culture filtrates were completely abolished by trypsin, but phospholipase C activity was resistant to inactivation. Lethal and phospholipase C properties of culture filtrates were resistant to inactivation at 45 C, whereas the hemolytic activity was completely destroyed. Lethal, hemolytic, and phospholipase C activities appeared simultaneously in a complex growth menstruum, but the kinetics of synthesis were different in all cases. Resolution of B. cereus filtrates on columns of Sephadex showed that the phospholipase C, hemolysin, and lethal toxin are distinct proteins. Evidence is also presented which suggests a correlation between the synthesis of B. cereus toxin and the period of transition from vegetative growth to sporulation. The activity of each B. cereus product was cation-independent, as opposed to cation-dependency of the phospholipase C and lethal activities of Clostridium perfringens alpha-toxin. Immunological cross-reactivity between the B. cereus products and C. perfringens alpha-toxin was not apparent; indeed, they were shown to be antigenically distinct.


Effect of adenosine monophosphate, adenosine diphosphate, and reduced nicotinamide adenine dinucleotide on adenosine triphosphate-dependent carbon dioxide fixation in the autotroph Thiobacillus neapolitanus. The observation that adenosine triphosphate (ATP)-dependent CO(2) fixation in extracts of chemosynthetic and photosynthetic autotrophs may be regulated in part by adenosine monophosphate (AMP) was extended to the strict autotroph Thiobacillus neapolitanus (X). In addition, this report presents data which include adenosine diphosphate (ADP) in the regulatory role. When the primary CO(2) acceptor, ribose-5-phosphate, was replaced by ribulose-1,5-diphosphate, no inhibition of CO(2) fixation occurred unless the Mg(++) concentration was limiting. A molar ratio of 5:1 AMP or ADP to ATP reduced the specific activity (micromoles of CO(2) fixed per milligram of protein per minute) of the extracts from 0.22 to 0.12 and 0.11, respectively. The reported stimulation of the carboxylative phase of ATP-dependent CO(2) fixation by reduced nicotinamide adenine dinucleotide (NADH(2)) was investigated. Adding NADH(2) to the extracts did not stimulate CO(2) fixation, even at carbonate levels from 0.05 to 30 mumoles, except in the absence of ribose-5-phosphate. Slight increases in CO(2) fixation were noted when the assay system was incubated in air instead of the usual helium atmosphere.


Fine structure of the cytomembranes of Nitrosocystis oceanus. Thin-sectioned, negatively stained, and freeze-etched preparations of Nitrosocystis oceanus cytomembranes were compared. The cytomembranes in freeze-etched cells were covered with 80- to 120-A particles. When cells were disrupted and differentially centrifuged, various membrane and particle fractions were obtained. Negatively stained membrane fragments from the pellet centrifuged at 3,000 x g showed 70- to 80-A stalked particles, whereas those from the pellet centrifuged at 39,000 x g exhibited a crystalline array of subunits with a 30- to 40-A periodicity. High-speed supernatant and pellet fractions centrifuged at greater than 39,000 x g contained 40- to 120-A free particles but no membranes. In chemically fixed cells, 40-A particles were found embedded in the matrix of membranes. Results suggest that the larger 80- to 120-A particles are enzyme complexes, whereas the smaller 30- to 40-A particles represent a structural protein or a lipoprotein of the membrane.


ENDOR study of irradiated histidine HCl.


Lysosomes and GERL in normal and chromatolytic neurons of the rat ganglion nodosum. The rat ganglion nodosum was used to study chromatolysis following axon section. After fixation by aldehyde perfusion, frozen sections were incubated for enzyme activities used as markers for cytoplasmic organelles as follows: acid phosphatase for lysosomes and GERL (a Golgi-related region of smooth endoplasmic reticulum from which lysosomes appear to develop) (31-33); inosine diphosphatase for endoplasmic reticulum and Golgi apparatus; thiamine pyrophosphatase for Golgi apparatus; acetycholinesterase for Nissl substance (endoplasmic reticulum); NADH-tetra-Nitro BT reductase for mitochondria. All but the mitochondrial enzyme were studied by electron microscopy as well as light microscopy. In chromatolytic perikarya there occur disruption of the rough endoplasmic reticulum in the center of the cell and segregation of the remainder to the cell periphery. Golgi apparatus, GERL, mitochondria and lysosomes accumulate in the central region of the cell. GERL is prominent in both normal and operated perikarya. Electron microscopic images suggest that its smooth endoplasmic reticulum produces a variety of lysosomes in several ways: (a) coated vesicles that separate from the reticulum; (b) dense bodies that arise from focal areas dilated with granular or membranous material; (c) "multivesicular bodies" in which vesicles and other material are sequestered; (d) autophagic vacuoles containing endoplasmic reticulum and ribosomes, presumably derived from the Nissl material, and mitochondria. The number of autophagic vacuoles increases following operation.


Influence of glucagon, an inducer of cellular autophagy, on some physical properties of rat liver lysosomes. The response of rat liver lysosomes to an intraperitoneal injection of glucagon has been evaluated from studies on the mechanical fragility, osmotic sensitivity, and sedimentation properties of these subcellular particles. It has been found that about (1/2) hr after the injection of glucagon the hepatic lysosomes exhibit a fairly sudden increase in their sensitivity to mechanical stresses and to exposure to a decreased osmotic pressure. At the same time, their sedimentation properties undergo complex changes characterized mainly by a significant increase in the sedimentation coefficient of a considerable proportion of the total particles. In addition, glucagon causes an increase in the proportion of slowly sedimenting particles, with the result that the distribution of sedimentation coefficients within the total population tends to become bimodal. The latter change is more pronounced for acid phosphatase, less so for cathepsin D, and barely detectable for acid deoxyribonuclease. All these modifications are maximal between 45 and 90 min after injection and regress to normal within approximately 4 hr. With the exception of the increase in the slow component, for which no explanation can be advanced at the present time, they are consistent with the hypothesis that glucagon causes an increase in lysosomal size, and may be related to the autophagic-vacuole formation known to occur after glucagon administration.


ESR studies of electron transfer: trans-stilbene-stilbene-system.


ESR of free-radical conversion in gamma-irradiated dihydrothymine.


Paget's disease of the scrotum. A case report.


The effect of lipid micelles on mitochondrial malate dehydrogenase.


The effect of Rous Sarcoma virus (Schmidt-Ruppin strain) on the chromosomes of human leucocytes in vitro.


Chromatography of polar triglycerides on silicic acid columns.


Temporary survival in clostridial hemolysis with absence of circulating red cells.


Screening of antibacterial agents for in vivo activity against pseudomonas infection.


Isolation of noninfectious particles containing Rous sarcoma virus RNA from the medium of Rous sarcoma virus-transformed nonproducer cells.


Deoxyribonucleoproteins from the ciliate Tetrahymena pyriformis GL.


Inactivation, by UV-, x-, and gamma-radiations, of the infecting and transforming capacities of polyoma virus.


DEAE-dextran: enhancement of cellular transformation induced by avian sarcoma viruses.


A viral interference test for mouse leukemia viruses.


Efficient, inefficient, and abortive infection of different mammalian cells by small RNA viruses.


An interferon antagonist: its effect on interferon action in mengo-infected Ehrlich ascites tumor cells.


Granular cell myoblastoma of the tongue.


Cancer in children--Wilms's tumor.

Cancer in children--Wilms's tumor.


Excretion of testosterone and 17-ketosteroids following administration of HCG and ACTH to normal adult males.


Metabolism of testosterone-4-C14 by rat brain homogenates and subcellular fractions.


Biosynthetic studies of human ovarian arrhenoblastomatous tissue in vitro. I. Sulfatase and sulfokinase activity.


Biosynthetic studies of human ovarian arrhenoblastomatous tissue in vitro. II. Formation of androgens from dehydroisoandrosterone sulfate.


Primary hepatoma 31 years after injection of thorotrast.


Krukenberg tumours in pregnancy.

Krukenberg tumours in pregnancy.


DPN (NAD-oxidized form): a preliminary evaluation in chronic schizophrenic patients.


Serological reactions in sheep pox. I. Complement fixation test.


Biological characteristics of primary tumors induced by polyoma virus in hamsters.


Absence of specific chromosome alterations in bovine lung fibroblasts exposed to Rous sarcoma virus.


Comparative study of angiotensin and nor-adrenaline in hypotensive states (shock).


Growth failure.

Growth failure.


A clinicopathological conference from the Ohio State University Hospital, Columbus, Ohio.


The epidemiology of Clostridium welchii food poisoning.


Trophocarcinoma of the testis. A report of eleven cases.


The use of dimethyl sulfoxide in the treatment of genitourinary disorders.


Diarrhea in infants: the role of viruses in the etiology of infantile  diarrhea.


Case notes. Value of ACTH in nonspecific optic neuritis.


Benign lumbosacral radicular pain; a neurosurgeon's approach.


Effects of adrenohypophyseal hormones upon urinary excretion of glucuronic acid and ascorbic acid.


Infectious bovine rhinotracheitis--a summary of the disease and its control with emphasis on New Zealand conditions.


Respiratory infection of cattle associated with Myxovirus parainfluenza 3.


Polyoma-like virions in a human demyelinating disease.


Mobility-molecular weight relationships of small proteins and peptides in acrylamide-gel electrophoresis.


Simultaneous onset of Addison's disease and diabetes mellitus. A case report.


Adrenergic blockade of hormone-induced lipolysis in isolated fat cells.


Plasma angiotensinase activity on angiotensin II and analogues.


Inhibition of mitochondrial respiration by progesterone and an azasteroid.


Reversal by methoxyethylmercury intoxication of NAD induced activation of glutamate dehydrogenase from rat liver.


Prostaglandins as adrenergic antagonists.


An enzymatic model for some effects of methyoxyethylmercury acetate on rat liver glutamate dehydrogenase.


Sodium sulphomethyl derivatives of polymyxins.


Effect of angiotensin on the release of acetylcholine from preganglionic and postganglionic nerve endings.


Factors affecting the incorporation of precursors into the phospholipids of Ehrlich ascites cells.


A study of the origin of vitamin-D from 7-dehydrocholesterol in fish.


Glycogenesis in the guinea pig liver during development.


The influence of the experimental conditions on the renal response to angiotensin in the rat.


The influence of perathiepine and chlorpromazine on some enzyme reactions in rat brain preparations.


Base composition of nonsense codons in Escherichia coli II. The N2 codon UAA.


The intralymphatic administration of radioactive isotopes in the overall treatment of malignant melanoma.


Inhibition of stromal enzymes by x-radiation.


Substrate specificity and stereospecificity of alcohol dehydrogenases.


Cyclic 3',5'-nucleotide phosphodiesterase: localization and latent activity in rat brain.


Catecholamine-secreting tumours.

Catecholamine-secreting tumours.


Steroids in gastrointestinal disease.

Steroids in gastrointestinal disease.


Susceptibility of the East African red-tail monkey kidney cells to Enteroviruses.


Treatment of nephrotic syndrome.

Treatment of nephrotic syndrome.


Hepatoblastoma presenting as an acute abdomen.


Enzymatic development of human fetus; studies on cytochrome oxidase and succinic dehydrogenase in developing human fetus.


Comparative study of nicotinamide adenine dinucleotide nucleosidase from Mycobacterium tuberculosis H37Rv & pig brain: effect of isonicotinic acid hydrazide on the enzyme-inhibitor complex.


Hepatoma with rupture and hemorrhage. Acute occult blood loss and abdominal pain in probable cirrhotic suggests diagnosis.


Transient QRS changes simulating myocardial infarction associated with shock and severe metabolic stress.


Degradation of oxytocin, lysine-vasopressin, angiotensin II and angiotensin-II-amide by oxytocinase (cystine aminopeptidase).


Glomus tumors. Postauricular hypotympanotomy and hypotympanoplasty.


Defective and delayed myxovirus infections.


The virus of infectious bovine rhinotracheitis in Northern Nigeria.


Photochemical formation of free radicals from aliphatic amines as studied by electron spin resonance.


Hazards of drugs for rheumatoid arthritis.


O-T-C depilatories.

O-T-C depilatories.


The use of corticosteroids and ACTH in allergic diseases.


Clostridium welchii bacteraemia: a case report with survival and review of the literature.


Effect of pyrophosphate on dissolution of hydroxyapatite and its possible importance in calcium homeostasis.


Characteristics of interferon induced in vitro and in vivo by a TRIC agent.


Brain-dependent ACTH secretion from multiple heterotopic pituitaries.


Human adenoviruses: tumor production in hamsters by type 12 and 18 grown from single plaques.


Studies on the mechanism of action of thymic leukemogenic virus.


Antibody responses in acute and chronic rubella.


Challenge virus resistance and interferon produced in BS-C-1 cells by dengue virus.


Effects of hypophysectomy and growth hormone on renal compensatory hypertrophy in rats.


ESR studies of model complexes for non-heme iron proteins.


Clinical syndromes associated with tumors of the pancreas.


Gustatory sweating.

Gustatory sweating.


Cellular resistance to induction of interferon.


Management of gestational trophoblastic disease.


Reversible inactivation of angiotensin in hypertensive and normotensive subjects.


Oxidation of testosterone acetate with per acids.


Evaluation of the short child.

Evaluation of the short child.


Neoplasms of the placental trophoblast.

Neoplasms of the placental trophoblast.


Management of status asthmaticus.

Management of status asthmaticus.


Angiotensin blood levels in normal and toxemic pregnancies.


Thin layer chromatography of toxicologically significant substances on silica gel-coated glass plates and polyester sheets.


Exposure of Pseudomonas aeruginosa to hyperbaric oxygen: inhibited growth and enhanced activity of polymyxin B.


A quantitative assay for avian myeloblastosis virus.


Metabolism of steroid hormones in adrenal cortex tumor cultures.


Steroid synthesis in embryonic and fetal rat adrenal tissue.


Effects of cortisol and ACTH administration on some aspects of lipid metabolism in the hamster.


Melanocyte-stimulating hormone-releasing and inhibiting factors in two hypothalamic extracts.


Effect of hypophysectomy and adrenalectomy on enzymes concerned with nicotinamide adenine dinucleotide synthesis.


A new micro-neutralization test for antibody determination and typing of parainfluenza and influenza viruses.


Transformation of hamster embryo cells in vitro by Murine Sarcoma Virus (Harvey).


Pituitary ACTH content in rats after partial or total interruption of neural afferents to the medial  basal hypothalamus.


Pyrophosphate phosphohydrolase in mammary tissue of  pregnant and lactating rats.


A new selective medium for Streptococcus pyogenes and other streptococci.


Effect of administration of ACTH on insulin secretion in dogs.


Clinical Pathologic Conference No. 3.

Clinical Pathologic Conference No. 3.


Patterns of antibodies to SV40 in children following the last booster with inactivated poliomyelitis vaccines.


Stability of antituberculosis drugs in Löwenstein-Jensen medium.


Electrodiagnosis. II. Peripheral nerve stimulation.


Viruses and human cancer.

Viruses and human cancer.


An investigation into thecomatosis of the ovary and related tumours by histochemical and enzyme histochemical methods.


Clinicopathological conference 83 conducted by the Department of Pathology, University of Mississippi School of Medicine, Jackson, Mississippi.


The isolation of viruses from acute respiratory infections. V. The use of organ cultures of human embryonic nasal and tracheal ciliated epithelium.


Plasma 17-hydroxycorticosteroid levels following intra-cranial self-stimulation in rats.


Canine herpesvirus infection of puppies.


Electron-spin resonance studies of photo-induced free radicals in native chloroplasts.


Ovarian neoplasms in childhood and adolescence.


Nerve abscesses in Northern Nigeria.

Nerve abscesses in Northern Nigeria.


Acute appendicitis diagnosed as carcinoma of the stomach.


Chemically characterized media for study of foot-and-mouth disease virus in baby hamster kidney cells. Foot-and-mouth disease virus can be grown in baby hamster kidney cells with a chemically characterized medium containing only tris(hydroxymethyl)-amino-methane (Tris) buffer, glucose, glutamine, and salts. Virus infectivity was only 0.5 log unit less than in a complex cell growth medium containing serum, tryptose phosphate, and lactalbumin hydrolysate. At high multiplicity of infection, production was maximal in 5 hr, with the virus remaining largely intracellular. Glucose and glutamine appeared to act independently of each other although both were required at about the same time during the virus production cycle. Glutamine had the greater effect and could not be replaced by amino acids, purines, and pyrimidines. Glutamine also stimulated cellular oxygen uptake in both normal and infected cells. Serum and other organic components added singly to the defined medium did not increase the virus yield. Studies on uninfected cells over a 5-hr incubation period showed that the defined medium maintained protein and ribonucleic acid synthesis at rates similar to the complex cell growth medium. These rates were much lower in media containing only inorganic salts and Tris buffer. Glucose, however, was more important to uninfected cellular metabolism than was glutamine. Defined medium containing dialyzed calf serum produced the highest rate of protein synthesis.


Histochemical comparison of dehydrogenases related to glucose metabolism in human tumors: localization and activity of lactate, glucose-6-phosphate, and 6-phosphogluconate dehydrogenases.


Neoplastic transformation of rat embryo cells by simian papovavirus SV40.


Recurrent herpes simplex ocular infection.


Jugularography as a diagnostic technique in otolaryngology.


Towards a rational chemotherapy of cancer.


Children with steroid-dependent allergic asthma.


Surgical management of benign intramural gastric tumors.


Electron paramagnetic resonance studies of vitamin K and vitamin E quinones.


The relationship between the virus of infectious canine hepatitis and inerstitial nephritis.


Corticosteroids and corticotropins in dermatological practice.


Three outbreaks of foodborne disease with dual etiology.


Biosynthesis of starch in chloroplasts.

Biosynthesis of starch in chloroplasts. The enzymic synthesis of ADP-glucose and UDP-glucose by chloroplastic pyrophosphorylase of bean and rice leaves has been demonstrated by paper chromatographic techniques. In both tissues, the activity of UDP-glucose-pyrophosphorylase was much higher than ADP-glucose-pyrophosphorylase. Glycerate-3-phosphate, phosphoenolpyruvate and fructose-1,6-diphosphate did not stimulate ADP-glucose formation by a pyrophosphorylation reaction. The major metabolic pathway for UDP-glucose utilization appears to be the synthesis of either sucrose or sucrose-P. On the other hand, a specific precursor role of ADP-glucose for synthesizing chloroplast starch by the ADP-glucose-starch transglucosylase reaction is supported by the coupled enzyme system of ADP-glucose-pyrophosphorylase and transglucosylase, isolated from chloroplasts. None of the glycolytic intermediates stimulated the glucose transfer in the enzyme sequence of reaction system employed.


Primary scirrhous carcinoma of colon. Roentgenologic diagnosis.


Carcinosarcoma of the esophagus.

Carcinosarcoma of the esophagus.


Trophoblastic malignancies.

Trophoblastic malignancies.


Intracellular collagen fibers. In human mesenchymal tumors and inflammatory states.


Recurrent cystosarcoma phylloides.

Recurrent cystosarcoma phylloides.


Adrenocorticotropins. 37. The synthesis of lysine 8-alpha-1-17NH 2-ACTH and its biological properties.


Synthesis of human angiotensin I.

Synthesis of human angiotensin I.


Photochemical formation of free radicals from hydrogen sulfide, mercaptans, and cysteine.


The kinetics of the periodate oxidation of substituted 2-aminoethanols.


Intra-oral presentation of lateral pharyngeal space tumors.


Correlations in the partition thin-layer chromatography of alkali metals.


Intraosseous glomus tumor. A case report.


Clinical study on primary carcinoma of the liver in Taiwan.


Current management glomus jugulare tumors.


Bisulphite addition compounds of periodate-oxidised polysaccharides: electrophoretic mobility, and precipitation by cetylpyridinium and other organic cations.


Studies on the relationship of the thermosensibility and sensibility to the acidic pH of certain cliones of foot and mouth disease virus obtained at infra-optimal temperature of 29 degrees C.


The overoxidation of carbohydrates with sodium metaperiodate.


Further studies of phosphatases in tissues and serum using gel-filtration techniques.


Cryotherapy in otorhinolaryngology.

Cryotherapy in otorhinolaryngology.


The present position of the treatment of parotid tumours.


Free radicals derived from phenothiazine.


The induction of cancer by viruses.

The induction of cancer by viruses.


P1, P2-pyrophosphates as initiators in the terminal deoxyribonucleotide transferase reaction. LA-3610-MS.


Fine structural alterations of Yoshida ascites hepatoma cells following treatment with L-erythro-alpha, beta-dihydroxybutyraldehyde.


Administration of corticotropin beta-1-24 as a rapid test of adrenal cortical function.


Fine structural localization of acid and alkaline phosphatases in cells of rabbit blood and bone marrow.


Clostridium perfringens septicemia as a postoperative complication of the newborn infant.


Hepatoma. A clinical and pathologic study of cases occurring during 1950-1959 in Hawaii.


Factors affecting filtration rates of flocculaed silica.


Vitamin D3--biologically active metabolites.


Studies in hypoglycemia of infancy and childhood. Diagnosis and treatment.


Sodium-potassium ratio in the urine after ACTH stimulation. A simple test of adrenocortical function.


Nonempirical calculations of the isotropic hyperfine splitting constants of 13-C, 14-N, and 17-O in simple radicals.


Paraplegia in childhood malignant disease.


Use of reagent-impregnated ("Patho-Tec") test papers in the identification of Enterobacteriaceae and similar bacteria.


Nature of the pressor substance in rabbit placenta. 1. The renin-like substance isolated from the placenta of the rabbit produces a prolonged increase of blood pressure in the nephrectomized rat. If incubated with renin substrate from ox serum, it forms a pressor substance that elevates blood pressure in exactly the same way as does angiotensin. 2. This angiotensin-like principle was concentrated by means of ion-exchange chromatography and compared with Val-5-angiotensins I and II in two paper-chromatography systems and in paper electrophoresis. 3. In all the three methods the unknown principle behaved like a mixture of the two reference compounds. 4. It is concluded that incubation of the renin-like substance of placental origin with substrate from ox serum gave a mixture of Val-5-angiotensins I and II. This is evidence that the enzyme isolated from the placenta is either closely related to, or identical with, renin.


Biochemical properties of anti-inflammatory drugs. VII. Inhibition of proteolytic enzymes in connective tissue by chloroquine (resochin) and related antimalarial antirheumatic drugs.


Some properties of the products of reaction of tadpole collagenase with collagen.


Incorporation of labeled precursors into lipids of tumors induced by Rous carcoma virus.


Biosynthesis of procollagen in rabbit sponge capsular tissue.


Interaction of Neurospora mitochondrial structural protein with other proteins and coenzyme nucleotides.


Zone electrophoresis of the proteins of avian myeloblastosis virus.


Chemodectomas of the head and neck.

Chemodectomas of the head and neck.


African swine fever. I. Antiserum production. These studies report on the production of African swine fever antiserum for use in serological tests. The first attempt to obtain antiserum was made by inoculating ASF virus - infected pig blood into the lactiferous sinus of lactating bovines. This failed to result in the development of detectable antibody, but resulted in propagation of the virus over a 14 to 21 day period. In the second attempt use was made of a tissue culture - attenuated virus to produce resistance in normal pigs. Clinical response to inoculation with the attenuated virus was limited to a one day increase of temperature. These pigs were subsequently orally exposed to virulent ASF virus and later challenged by intramuscular injection. The sera were subjected to testing by the modified direct complement-fixation test and the agar gel double-diffusion technique in order to follow the development of antibodies. Some sera were also conjugated with fluorescein isothiocyanate and used for the detection of viral antigen by the fluorescent antibody technique. It was found that inoculation with the attenuated virus brought about the development of low antibody levels in the pigs. This antibody level did not increase following oral exposure. One pig following intramuscular challenge underwent a series of ascending temperature peaks, coinciding with increased complement-fixing titres.


Induction of tumours in mice with three variants of rous sarcoma virus and studies on the immunobiology of these tumours.


Characteristics of highly polyploid clones isolated from lines of RSV-transformed rat cells.


Isolation and basic characteristics of template active RNA of BAI strain A virus (avian myeloblastosis).


Sarcomas in reptiles induced with Rous virus.


Adaptation of L cells and of lines of virogenic rat cells transformed by Rous sarcoma virus to growth in protein-free synthetic medium.


Electron microscopy of size and shape of viral DNA in solutions of different ionic strengths.


State junior colleges and the preparation of nurses.


Joint statement on cardiopulmonary resuscitation issued by Minnesota Nurses Association, Minnesota State Medical Association and Minnesota Hospital Association.


Joint statement on I.V. administration of fluids issued by Minnesota Nurses Association, Minnesota State Medical Association, and Minnesota Hospital Association.


Zyloprim and other drugs in the management of gout.


Atypical reaction to inhaled silica.

Atypical reaction to inhaled silica.


Possible synergic influence of ACTH and FSH on the adrenal cortex in hypophysectomized-gonadectomized rats.


The binding sites of iron in rubredoxin from Micrococcus aerogenes.


Mapping the 5'-terminal nucleotides of the DNA of bacteriophage lambda and related phages.


Stimulating interaction between viruses (stimulons).


Specific activation of the DNA-synthesizing apparatus in contact-inhibited mouse kidney cells by polyoma virus.


Experimental virus pneumonia in lambs.

Experimental virus pneumonia in lambs.


Comprehensive treatment of acute hemorrhagic pancreatitis.


The effects of ionizing radiation on nucleic acids of bacteriophages and bacterial cells.


Virus leukemia in the mouse.

Virus leukemia in the mouse.


Neurologic manifestations of Hodgkin's disease. Intracerebral Hodgkin's granuloma.


Blind pancreatic resection for suspected insulinoma: a review of the problem.


Glomus jugulare tumours with special reference to their radiological features.


Postoperative gas gangrene.

Postoperative gas gangrene.


Chemodectoma in the region of the aortic bifurcation.


Beta-hydroxybutyrate dehydrogenase activity in liver and liver tumors.


The in vitro soluble RNA methylase activity of SV40-induced hamster tumors.


The neonatal and infant age periods as biologic factors which modify multicarcinogenesis by urethan.


Stimulation of DNA synthesis and thymidine kinase activity in human embryonic kidney cells infected by Adenovirus 2 or 12.


Amino acids of the ornithine cycle in transformed hamster fibroblasts carrying pleuropneumonia-like organisms.


Post-hospital contacts with schizophrenics.


Variola minor. Observations and experiences in Staffordshire, England, during 1966.


Effects of angiotensin and renal artery constriction on erythropoietin production.


Cylindroma (adenoid cystic carcinoma) of the upper air passages. Two case reports.


Cutaneous flushing as a primary manifestation of functioning insulinoma: report of a case.


Thyroid stimulating and pigmentary effects of synthetic peptides related to alpha-MSH and ACTH.


The dimple sign in peroneal palsy.

The dimple sign in peroneal palsy.


Human collagenase: identification and characterization of an enzyme from rheumatoid synovium in culture. Synovial tissue from patients with rheumatoid arthritis produces lysis of gels of reconstituted collagen fibrils in culture and releases soluble collagenase when cultured in collagen-free medium. Collagen molecules in solution at neutral pH at 20 degrees and 27 degrees C are cleaved by the synovial enzyme into (3/4) and (1/4) length fragments. In this respect the action of synovial enzyme is similar to that of amphibian collagenase and distinct from that of bacterial collagenase. At 37 degrees C reconstituted collagen fibrils and native fibers are attacked by the enzyme and further degraded to polypeptides of low molecular weight. These polypeptides are produced only after denaturation of the larger fragments, which occurs at temperatures near 37 degrees C.


Management of trophoblastic disease at the University Hospital.


Medical grand rounds from the University of Alabama Medical Center.


Portal hypertension: current status of surgical management.


Uremic polyneuropathy: and motor nerve conduction velocities.


Parabiotic dialysis.

Parabiotic dialysis.


Enzyme histochemistry of the angle of the anterior chamber of the human eye.


A method for sequential quantitative separation and determination of protein, RNA, DNA, lipid, and glycogen from a single rat liver homogenate or from a subcellular fraction.


Use of a sensitive new method for determination of the urinary excretion of dichlorodiphenylacetic acid (DDA) by human subjects.


Determination of phosphate in biological materials and reaction mixtures by atomic absorption spectrophotometry.


Determination of intracellular 2-deoxy-D-glucose 6-phosphate and uridine diphosphate-2-deoxy-D-glucose.


A gas chromatographic method for analysis of anomeric carbohydrates and for determination of mutarotation coefficients.


Detection and differentiation of sugars on paper by the p-anisidine-periodate reaction.


Microenzymic method for the quantitative analysis of acetyl groups in proteins and peptides.


Electron transport systems of yeast. IV. Preparation and properties of a particulate DPNH, succinate cytochrome c reductase.


Effect of isoniazid on preservation of nicotinamide dinucleotide in animal cells.


Effect of N1-alkylnicotinamide chlorides on glutamate oxidation in rat liver mitochondria.


Enzymic polymerization of monosaccharides. I. The enzymic polymerization of D-galactose.


Enzymic polymerization of monosaccharides. II. Synthesis of 2-acetamido-2-deoxy-6-O-(alpha-D-galactopyranosyl)-D-glucose (N-acetylmelibiosamine).


Demonstration of the Schiff base mechanism in an aldolase reaction by oxygen exchange.


Optical activity of hematin A.

Optical activity of hematin A.


A purified plasma membrane fraction isolated from rat liver under isotonic conditions.


Techniques for analysis of glucose binding by human erythrocyte membranes.


Studies on the mechanism of cyclization of glucose 6-phosphate to D-inositol 1-phosphate.


Biosynthesis of nicotinamide-adenine dinucleotide in Escherichia coli.


On the mode of action of 7-deaza-adenosine (tubercidin).


Stability of nucleolar 45-S RNA.

Stability of nucleolar 45-S RNA.


Magnetic susceptibility measurements on hemoproteins down to 4.2 degrees K.


2,3-Dihydroxybenzoate as a bacterial growth factor and its route of biosynthesis.


Polyanions and their complexes. II. Light-induced paramagnetism in solid glycosaminoglycan-dye complexes.


Controlled evaluation of nicotinamide adenine dinucleotide in the treatment of chronic schizophrenic patients.


Effects of intravenous administration of adrenocorticotrophin on the adrenal lipids of the rabbit.


Acute ascending polyradiculomyelitis. Associated with ECHO 9 virus.


Spores of microorganisms. XXI. Conversion of outgrowing spores of Bacillus cereus to refractile forms by basic peptides and proteins.


Nucleic acids. I. The synthesis of nucleotides and dinucleoside phosphates containing ara-cytidine.


The structure-activity relationship in barbiturates and its similarity to that in other narcotics.


Nucleic acids. 3. Antiviral activity of nucleotides and dinucleoside phosphates containing ara-cytidine.


Synthesis and antiviral and antibacterial activity of certain N-dialkylaminomethylisatin beta-thiosemicarbazones.


Changes of mitochondrial RNA level during the transition from rest to growth in the endosperm of germinating castor beans.


Pathways of glucose utilization in kidney homogenates of tuberculous and isoniazid-treated tuberculous guinea pigs. (Preliminary report).


Histogenesis of the granular-cell myoblastoma.


Incidence of cytomegalic infection in man.


The implication of acid concentration in lipid phosphorus determinations.


Detection and determination of 4-hydroxyskatole sulfate ester.


A direct spectrophotometric assay for pyruvate kinase.


Spontaneous and detergent activation of a glucuronyltransferase in vitro.


Incorporation of 2-deoxyglucose into glycogen by intact Novikoff ascites hepatoma.


A comparative study of glycogen and lactate formation from glucose, glucose 6-phosphate, alpha-glucose 1-phosphate, and uridine diphosphate glucose by intact Novikoff ascites-hepatoma cells.


Simultaneous binding of coenzyme-competitive inhibitors of yeast alcohol dehydrogenase.


Specific cleavage of tryptophyl peptide bonds with periodate in sperm whale myoglobin.


"Early, non-virion" antigens in herpes simplex virus infected tissue culture cells. I. Detection of "early" antigens with complement fixation test.


Ion accumulation in bacterial systems. I. Isolation of two particulate fractions participating in silicon metabolism, from Proteus mirabilis cell-free extracts.


Ion accumulation in bacterial systems. II. Properties of silicate-metabolizing cell-free extracts and particulate fractions from Proteus mirabilis.


On the potentiation of kinins by sulfhydrylic compounds.


Further studies on noradrenaline postinfusional hypotension.


The investigation of hypopituitarism.

The investigation of hypopituitarism.


An evaluation of screening procedures in the diagnosis of reno-vascular hypertension.


The response of the blood pressure to an infusion of angiotensin in patients with essential malignant and renovascular hypertension.


The action of trypsin and of chymotrypsin on phenylthiocarbamyl-angiotensin II.


Effects of serum proteins in vitro on the synthesis of rapidly labelled ribonucleic acid and on the polysome pattern of yoshida ascites-hepatoma cells.


A method for the study of bone mucosubstances by using collagenase.


Conversion of reduced nicotinamide-adenine dinucleotide dehydrogenase into reduced nicotinamide-adenine dinucleotide-ubiquinone reductase.


Isopentyl pyrophosphate isomerase from pig liver.


Polyoma-virus-induced deoxyribonucleic acid polymerase activity in resting mouse-embryo cells.


Oxidative phosphorylation and respiratory control in mitochondria from Aspergillus niger. 1. Tightly coupled mitochondria were isolated from Aspergillus niger by using an all-glass homogenizer followed by differential centrifugation. 2. The mitochondria oxidized the common intermediates of the tricarboxylic acid cycle, NADH(2) and the ascorbate-tetramethyl-p-phenylenediamine system. 3. High P/O ratios and control of respiration by ADP were obtained with all substrates tested. The average P/O ratios observed were: 1.5-1.8 with succinate as substrate [respiratory control ratio (RC) 2-4]; 0.8-1.0 with ascorbate-tetramethyl-p-phenylenediamine (RC 1.2-1.5); 1.4-1.8 with NADH(2) (RC 2-3); 2.4-2.8 with alpha-oxoglutarate (RC 3-5). 4. Bovine serum albumin (0.05-0.2%) was essential for tightly coupled respiration to be observed. 5. Coupled oxidation of exogenous NADH(2) was relatively insensitive to rotenone and Amytal. 6. The mitochondria responded to specific inhibitors and uncoupling agents in a manner similar to that of mammalian mitochondria. 7. It was concluded that the isolated mitochondria from A. niger show respiratory properties similar to those reported for intact yeast and mammalian mitochondria.


The inhibition of skeletal-muscle fructose 1,6-diphosphatase by adenosine monophosphate. 1. The present study extends the finding of Krebs & Woodford (1965) that muscle fructose diphosphatase is more sensitive to AMP inhibition than liver fructose diphosphatase. 2. Hen breast fructose diphosphatase has a K(i) for AMP of 0.1mum; the plot of percentage inhibition is non-sigmoid and the reciprocal plot of activity against AMP concentration is sometimes linear. 3. Percentage inhibition plots for other muscle fructose diphosphatases are sigmoid curves which exhibit different threshold responses to the AMP concentration. 4. The intracellular content of AMP in all muscles tested exceeds the inhibition concentration range of AMP. 5. The sensitivity of muscle fructose diphosphatase to AMP inhibition is decreased by the presence of Mg(2+) or Mn(2+) ions; in the presence of Mn(2+) the inhibition curve for hen breast fructose diphosphatase becomes sigmoid. 6. From the formation constants for the Mg(2+) and Mn(2+) chelates, the effect of these ions in chelation of AMP can be calculated. Although chelation of AMP can explain the Mg(2+) effect, it cannot explain the marked relief of AMP inhibition by Mn(2+). 7. It is suggested that Mn(2+) has a specific effect on this enzyme which reduces the sensitivity to AMP inhibition.


A comparison of the properties of fructose 1,6-diphosphatase, and the activities of other key enzymes of carbohydrate metabolism, in the livers of embryonic and adult rat, sheep and domestic fowl. 1. The activities of some key enzymes of glycolysis and gluconeogenesis were measured in embryonic chick, sheep and rat livers. 2. In chicken the activities of hexokinase, phosphofructokinase and pyruvate kinase are low, but those of glucose 6-phosphatase and fructose diphosphatase are very high; the converse situation exists in the rat (Burch et al. 1963), but in sheep the activities of both phosphofructokinase and fructose diphosphatase are high, and the activities of hexokinase and glucose 6-phosphatase are low. These findings are discussed in relation to carbohydrate metabolism in these embryonic livers. 3. The regulatory properties of fructose diphosphatase from the embryonic livers of these three species were compared with the properties of the enzymes from adult animals. The inhibitions by AMP and fructose diphosphate and the effects of Mg(2+) and pH on the activities of adult and foetal fructose diphosphatase are almost identical. 4. It is concluded that regulatory properties are characteristic of fructose diphosphatase from embryonic and adult tissue, and the importance of this in relation to enzyme development is discussed.


Polyribosome breakdown in rat liver following administration of 8-azaguanine.