Status epilepticus partialis wit fear as clinical expression. Report of a case and ictal EEG findings.


HEW: be not dismayed.

HEW: be not dismayed.


Student self-assessment in dental technology.


Method for decreasing subjective evaluation in preclinical restorative dentistry.


Two problems confronting continuing education in dentistry: local or national problems?


A clinical evaluation instrument in endodontics.


Defining clinical competence in terms of quality, quantity, and need for performance criteria.


Design of private practice preceptorship program.


Helium: absence of antiarrhythmic effect in anesthetized dogs.


Chronic granulocytic leukemia in children.


Radiology quiz.

Radiology quiz.


Leukapheresis in the management of chronic leukemia.


Stereochemical interpretation of high oxygen affinity of haemoglobin Little Rock alpha-2beta-2 143His-Gln.


Plasma lipids and atherosclerosis.

Plasma lipids and atherosclerosis.


Diet and triglyceride metabolism.

Diet and triglyceride metabolism.


Molecular structures of acetylcholinesterase from electric organ tissue of the electric eel. Three purified molecular forms of acetylcholinesterase (EC 3.1.1.7) with sedimentation coefficients of 18 S, 14 S, and 11 S were studied by analytical ultracentrifugation and electron microscopy. The three species have molecular weights of (1.1 +/- 0.1) x 10(6), (7.5 +/- 1.5) x 10(5), and (3.35 +/- 0.25) x 10(5), respectively. Electron micrographs reveal that the 18S and 14S forms are asymmetric, composed of a head, containing a large number of subunits, and an elongated tail. The 11S form of acetylcholinesterase is apparently a tetrameric structure devoid of the tail. Maleylation of 18S and 14S acetylcholinesterases abolishes their tendency to aggregate at low ionic strength.


Hypolipoproteinaemia.

Hypolipoproteinaemia.


Cystic fibrosis and coeliac disease: coexistence in two children.


Adhesive recognition and retinotectal specificity. An assay has been developed to test the hypothesis that neurons from a limited area of the retina will adhere preferentially to that part of the optic tectum near their normal synaptic termini. The method measures the adherence of (32)P-labeled cell bodies from either the dorsal or ventral half of the neural retina of chick embryos to dorsal and ventral tectal halves. When a labeled, single-cell suspension is prepared from dorsal half-retina, more cells bind to the ventral half of the tectum. When the labeled cells are from ventral half-retina, more bind to dorsal half-tecta. This preferential adhesion mimics the retinotectal projection found in vivo. Dorsal retinal cells show this preference shortly after dissociation with crude trypsin, and maintain it for at least 9 hr. Ventral retinal cells, however, require incubation in nutrient medium after trypsinization in order to display this selectivity. Comparable results are obtained when the cell suspension is prepared from pigmented retina. The data support an interpretation of neuronal specificity dependent on cell-surface interactions and demonstrate a clear correlation between selective adhesion and biological function.


Today's approach to protecting employees from the heavy metals.


Evidence for higher functions of the cerebellum: eating and grooming elicited by cerebellar stimulation in cats. Well-organized eating and grooming behaviors were elicited in cats by stimulation of a zone in the cerebellum that extended from the fastigial nucleus to the superior cerebellar peduncle. Behaviors appeared to result from the facilitation of specific sensorimotor mechanisms, rather than the induction of generalized "drive" states. The results emphasize the need for a broad view of cerebellar function.


Radio-frequency energy: a hazard to workers?


Temperature-induced variations in the surface topology of cultured lymphocytes are revealed by scanning electron microscopy. Temperature-induced variations in the surface morphology of cultured lymphocytes were evaluated by scanning electron microscopy. At 25-37 degrees the cells' surfaces are largely obscured by numerous undulating microvilli of various lengths but uniform diameter. Temperature changes alter the number of microvilli, their lengths, diameters, distribution, branching, and fusing. Typically, chilling to 0-4 degrees markedly reduces the number of microvilli and increases the diameter of the survivors in a reversible process. In contrast, heating the cells to about 45 degrees rapidly and irreversibly transforms the ordinarily smooth membrane surface into one with a "cobblestone" morphology. At the same time most microvilli disappear and the few that remain clump into a cap. The data suggest that the low-temperature effects reflect a change in the physical state of membrane lipids, while the high-temperature alterations represent thermotropic protein transitions.


Two distinct types of enhancement of galactose uptake into hamster cells: tumor-virus transformation and hexose starvation. Enhancement of hexose uptake seems well correlated with transformation of cell cultures by tumor viruses and the absence of contact inhibition. Enhancement of sugar uptake has also been observed as a result of hexose starvation. Both types of enhancement can clearly be demonstrated in cultures of hamster cells when uptake of (14)C-labeled galactose is monitored after 10 or 20 min. The profiles of accumulation products are strikingly different. In cultures of hamster NIL cells transformed with polyoma virus much of the (14)C is accumulated as UDPhexose. Untransformed cells accumulate galactose-l-phosphate as well as UDPhexose. Hexose-starved cells show enhanced uptake of galactose; however, this marked enhancement was only observed in NIL cultures close to contact inhibition. The novel and common feature seen in hexose-starved cells when incubated briefly with (14)C-labeled galactose is the occurrence of a marked accumulation of [(14)C]UDPglucuronic acid at the expense of UDPhexose. The ratio [(14)C]UDPglucuronic acid/UDPhexose in cultures fed glucose or galactose was invariably low (0.15-0.2) regardless of the presence or absence of contact inhibition. 20 hr of hexose starvation invariably changed this ratio by a factor of 10 or more, due to accumulation of UDPglucuronic acid. This result was also observed in cultures transformed with polyoma virus. The presence of 3-O-methylglucose in the growth medium did not alter the typical "sugar starvation pattern" (i.e., the UDPglucuronic acid/UDPhexose ratio averaged 1.7). Enhancement of galactose uptake by hexose starvation was very pronounced in NIL cultures that were close to contact inhibition, but was not a prominent feature in the polyoma-transformed cultures. The transformed cells grown on glucose or galactose growth medium showed the usual enhanced rate of uptake of galactose as compared with nontransformed near-confluent cultures that had been fed hexose. The polyoma-induced enhancement showed none of the features characteristic of hexosestarved cells.


New lymphocyte antigen system (Lna) controlled by the Ir region of the mouse H-2 complex. A new system of lymphocyte alloantigens in mice is described. This Lna (lymph-node antigen) system is associated with the Ir region of the H-2 (histocompatibility-2) gene complex. It has the following distinctive characteristics: (1) The gene or genes controlling these antigens has been mapped in the Ir (immune response) region between H-2K and Ss-Slp. (2) The antigens are most readily detectable on lymph-node cells, although they are also expressed on peripheral blood lymphocytes, splenic lymphocytes, and thymocytes. (3) Cytotoxicity against only about half of lymph-node cells is consistently observed. (4) Cytotoxic antibody titers against these antigens are strikingly high-more than 2000 by (51)Cr-release and up to 100,000 in the microcytotoxic test. (5) At least two, probably allelic, forms of the antigen(s) have been defined, one associated with the H-2(k) haplotype and one with the H-2(a) haplotype. (6) Antisera against Lna contain multiple antibody specificities that can be fractionated by absorption either with certain recombinants or with other H-2 halotypes that have crossreactive antigens. The antisera against Lna may be of value for definition and characterization of the products of the Ir and MLR (mixed lymphocyte reaction stimulatory) genes associated with the H-2 complex.


Transformation of neuraminidase-treated lymphocytes by soybean agglutinin. Transformation of mouse-spleen lymphocytes in the presence of soybean agglutinin is markedly enhanced after their treatment with neuraminidase (EC 3.2.1.18). Incubation of the cells with the enzyme also facilitates their agglutination by the lectin. The soybean agglutinin-induced agglutination and transformation is inhibited specifically by N-acetyl-D-galactosamine. New specific binding sites for soybean agglutinin were shown to appear after neuraminidase treatment. It is postulated that the transformation of neuraminidase-treated mouse-spleen lymphocytes induced by soybean agglutinin is caused by the binding of the lectin to galactosyl residues exposed by the action of neuraminidase on the cell membrane.


Binding of nerve growth factor receptor in sympathetic ganglia. (125)I-Labeled nerve growth factor binds to membrane preparations from superior cervical ganglia of rabbit in a specific fashion indicative of an interaction with the physiological receptor. Of various tissues examined, binding was observed only in the superior cervical ganglia, a presumed target organ of nerve growth factor. Binding of labeled factor is displaced by nonradioactive factor but not by several other peptide hormones. Binding to ganglia of preparations of nerve growth factor treated with N-bromosuccinimide closely parallels their biological activity. Binding of nerve growth factor receptor is saturable with an affinity constant of about 0.2 nM, similar to the reported plasma levels of the factor. Nerve growth factor does not elicit insulin-like biological effects in isolated adipocytes, and it does not modify the binding of [(125)I]insulin or [(125)I]epidermal growth factor to fat cells or liver membranes.


Developmentally regulated, carbohydrate-binding protein in Dictyostelium discoideum. A carbohydrate-binding protein assayed by its ability to agglutinate formalinized sheep erythrocytes is synthesized between 3 and 9 hr after Dictyostelium discoideum cells are deprived of food, as the cells become cohesive. Agglutination of erythrocytes by this protein was inhibited by N-acetyl-D-galactosamine, D-galactose, and L-fucose, but other monosaccharides had little or no effect. The protein bound completely to Sepharose 4B, and was isolated in highly purified form by elution with D-galactose. It appears to be present on the surface of cohesive but not vegetative slime-mold cells. The possibility that this protein may mediate intercellular adhesion in Dictyostelium is considered.


Some binding parameters of chromatin acidic proteins with high affinity for deoxyribonucleic acid. A fraction of chromatin acidic protein from rat liver, predominantly comprised of low-molecular-weight species, showed highest in vitro affinity for DNA when compared with other fractions of nuclear acidic protein. Maximum binding of this protein fraction was observed at physiological ionic strength of 0.14 M NaCl and DNA/protein ratio > 1. Comparison of their binding to homologous and heterologous DNAs showed partial species specificity. All molecular species of this protein fraction appear to bind to DNA.


Separation of the messenger RNAs of Newcastle disease virus by gel electrophoresis. We have separated the 18-22S putative messenger RNA of Newcastle disease virus into seven species ranging in molecular weight from 0.55 to 1.53 x 10(6) using sodium dodecyl sulfate-acrylamide-gel electrophoresis at relatively high concentrations of acrylamide and for a relatively long time. Studies of the number and molecular weights of the proteins and the 18-22S RNAs of the virus suggests that these RNAs are in the right molecular weight range to code for the known proteins of Newcastle disease virus. In preliminary studies using this separation technique, we have demonstrated that: (a) there is no difference between the 18-22S RNA made during a normal infection and when genome replication is blocked; and (b) there is a strain-specific difference between the RNAs of Newcastle disease virus-AV and Newcastle disease virus-HP.


Guanosine 5'-diphosphate, 3'-diphosphate: assignment of structure by 13C nuclear magnetic resonance spectroscopy. Guanosine tetraphosphate, recently discovered to mediate the regulatory relationship between protein synthesis and RNA accumulation in various bacteria, has been synthesized in vitro in large quantities and analyzed by natural-abundance (13)C nuclear magnetic resonance spectroscopy in order to confirm its structure and establish the positions of phosphate attachment. These studies have established its structure as guanosine 5'-diphosphate, 3'-diphosphate.


The mechanism of photoaffinity labeling. Photoaffinity labeling is a recently introduced method for covalently binding chemical tags to the active sites of protein molecules, which is potentially capable of very great specificities of labeling. A labeling reagent is used that is converted by photolysis to an extremely reactive intermediate. According to the expected mechanism, the reagent molecules that are specifically and reversibly bound to the active site at the instant of photolysis react irreversibly in the site before they can dissociate from the site. In two such reagent-protein systems studied in this paper, however, it is shown that, while by the usual criteria photoaffinity labeling appears to have occurred, the expected mechanism in fact does not hold. This was discovered in experiments with scavengers present in the mixtures that were photolyzed. The general properties of, and criteria for, photoaffinity labeling reactions are discussed in the light of these findings.


Synthesis of the myoglobin active site.

Synthesis of the myoglobin active site. A simple heme-imidazole compound, having the same geometry as the heme-imidazole complex in myoglobin, has been synthesized. This compound, ferropyrroporphyrin-N-[3-(1-imidazolyl)propyl]amide, reversibly binds oxygen in the solid state or when dissolved in a polystyrene film. These results suggest that the principal factors governing reversible oxygen binding are the electronic nature of the base (imidazole), neighboring-group effects of the basic group, and immobilization of the heme group.


Pyridoxal compounds as specific reagents for the alpha and beta N-termini of hemoglobin. Bifunctional pyridoxal derivatives with a charged side chain in the 5' position react specifically with the N-terminal valine residues of hemoglobin. Three of these compounds, which have only a single negative charge in the side chain, are highly selective for the alpha-chain N-terminal residues in the oxy conformation while pyridoxal phosphate, with two negative charges, reacts specifically with the beta N-terminal in deoxyhemoglobin. Schiff's base formation, therefore, results in decreased oxygen affinity with pyridoxal phosphate but increased oxygen affinity with the other three compounds. Reduction with sodium borohydride leads to irreversible coupling at either end of the molecule, and preparation and properties of such derivatives are described.


Alignment of two DNA helices: a model for recognition of DNA base sequences by the termini-generating enzymes of phage lambda, 186, and P2. Based on the 3'- and 5'-terminal sequences of DNA of phage lambda, P2, and 186, a model is proposed for recognition of DNA sequences by enzymes responsible for generation of cohesive ends. Two copies of the cohered ends, either on separate molecules or on a concatemer, are aligned with their helical axes parallel but running in opposite directions. The nicking system is dimeric, with each of the two monomers carrying identical sequence-recognition sites. Two pairs of nicks are introduced into the two aligned DNA molecules by the nicking system. The applicability of this model to other biological processes, such as integration of a viral genome into a host genome and the cutting of concatemeric T7 DNA, is discussed.


Maximal concanavalin A-specific agglutinability without loss of density-dependent growth control. Density-inhibited 3T3 mouse fibroblasts were treated for 10 min with nine concentrations of Pronase ranging from 0.25 to 40 mug/ml. Concanavalin A-specific agglutinability increased from control values of about 25% to plateau values of about 80%. None of these Pronase concentrations brought about an increase in cell number within 84 hr. Pronase-treated cells remained responsive to growth stimulation by serum and cortisol. Therefore, the protease-mediated surface change measured by increased concanavalin A-specific agglutinability is not an event sufficient by itself to bring about cell division.


Regulation of 5-methyltetrahydrofolate: homocysteine methyltransferase activity by methionine, vitamin B12, and folate in cultured baby hamster kidney cells. Rapid growth of BHK cells in methioninedeficient medium required supplementation with homocysteine, B(12), and over 40-fold greater levels of folic acid than growth in methionine-supplemented medium. The activity of the B(12)-dependent 5-methyltetrahydrofolate: homocysteine methyltransferase was studied in extracts of BHK cells grown in media containing various concentrations of the compounds of the enzyme reaction. The methyltransferase activity increased over 4-fold when B(12)-deficient deficient medium was supplemented with optimal levels of B(12); this increase was not prevented by puromycin. Addition of homocysteine to growth medium containing methionine, B(12), and folic acid was without effect. However, methyltransferase activity increased 2.5- to 4.0-fold further beyond the highest levels obtained in the presence of methionine, B(12), and folic acid when homocysteine was substituted for methionine in the growth medium. This increase was blocked by puromycin and was not due to removal of feedback inhibition of activity by the product methionine. These results suggest that methyltransferase activity may be regulated in part by derepression of the enzyme's synthesis on substitution of the substrate homocysteine for the product methionine.


Nucleotide sequence of HS-beta satellite DNA from kangaroo rat Dipodomys ordii. The sequence of the highly repetitive satellite HS-beta DNA fraction from kangaroo rat Dipodomys ordii was determined independently by RNA and DNA sequencing techniques. A basic iterated sequence of 10 nucleotides with several mutational variations was found. Base-composition data are consistent with the proposed sequence and revealed a high content of 5-methylcytosine. DNA and RNA sequencing techniques used gave identical results, showing that the fidelity of synthesis of riboguanidine-substituted DNA under our conditions is adequate for nucleotide sequence studies.


A specific photoreaction in polydeoxyadenylic acid. Ultraviolet photosensitivity of the single-stranded forms of poly(A) and poly(dA) is compared. In contrast to poly(A), poly(dA) is rather sensitive to ultraviolet irradiation. Relatively low doses of ultraviolet light lead to characteristic changes of the ultraviolet and circular dichroism spectra of poly(dA), suggesting formation of a specific photoproduct in poly(dA) that cannot be formed in poly(A). The quantum yields in poly(dA) (varphi = 2.5 x 10(-3)) are about a factor of 10 higher than in poly(A), indicating a high structure specificity. The base-pairing capacity of irradiated probes of poly(dA) to poly(dT) is inhibited. These results demonstrate a relatively high probability of DNA photolesions, that cannot be repaired by simple repair enzyme systems.


General homeostasis of the frequency of circadian oscillations. Some well-defined statistical regularities characterize the change in period (tau) of cockroach circadian oscillations subjected to a large temperature step. These are explainable in terms of the well-known temperature-compensation (homeostasis) of tau of circadian oscillations. The same regularities are detectable in published data on the effect of several other variables affecting several other circadian oscillations. The proposition is then developed that the temperature-compensation of tau is only a special case of a general homeostatic conservation of the frequency of circadian oscillations in the face of all changes they are likely to encounter in the cell. Such a general homeostasis of tau is a functional prerequisite for an oscillator to function as a useful "clock."


Complete amino-acid sequence of actin of rabbit skeletal muscle. The complete amino-acid sequence of actin of rabbit skeletal muscle was determined. The actin polypeptide chain is composed of 374 residues, including one residue of the unusual amino acid N(r)-methyl histidine, and has a calculated molecular weight of 41,785. The sequence of actin was determined by isolating the peptides produced by cleavage of the protein with cyanogen bromide, determining the sequence of these peptides, and establishing their order within the molecule. This study represents the first complete determination of the aminoacid sequence of a myofibrillar protein. Comparison of this sequence with peptides from actins isolated from different sources indicates that the sequence of actin is highly conserved.


Radioautographic studies of krypton-85 clearance from rat incisor pulp and surrounding tissues.


Stimulation of polypeptide initiation in vitro after protein synthesis inhibition in vivo in HeLa cells. Crude cytoplasmic extracts prepared from HeLa cells actively incorporate amino acids but show little initiation of new peptides (as seen by labeling of N-terminal amino acids). In contrast, extracts prepared from cells subjected to prior inhibition of protein synthesis show a significant amount of polypeptide initiation indicated by formation of peptides with radioactive N-terminal methionine. The same result was obtained whether prior inhibition occurred with cycloheximide or by starvation for an essential amino acid. Cellular response to suppression of protein synthesis appears to be mediated through production of RNA, since it is inhibited by actinomycin but appears in the presence of cycloheximide. The crude extracts continue initiating new polypeptides for at least 10 min in vitro. It is postulated that enhancement of in vitro initiation described here is related to the apparent stimulation of initiation of translation seen in vivo.


The mechanism of uptake of 18F by enamel from sodium fluoride and acidulated phosphate fluoride solutions labelled with 18F.


The composition of stimulated human parotid saliva.


The prevalence of human dental caries and water-borne trace metals.


Circadian rhythms in the flow rate and proportional contribution of parotid to whole saliva volume in man.


The existence of glycosylated and non-glycosylated forms of human submandibular amylase.


The biochemical growth and development of the palate, maxilla, mandible and tongue in the New Zealand white rabbit.


Excitatory and inhibitory influences from laryngeal and orofacial areas on tongue position in the cat.


Circadian rhythms in human parotid saliva flow rate and composition.


The effects of avulsion of the inferior alveolar nerve on the mandibular canal of the albino rat.


The effect of tooth wear on the distribution of fluoride in the enamel surface of human teeth.


Prevalence of oral leukoedema in Uganda.


Moebius' syndrome. Surgical management of a case.


Anatomical configurations in dry mandibles.


Traumatic internal carotid artery occlusion following fracture of the mandible.


Mobility of carbohydrate-containing structures on the surface membrane and the normal differentiation of myeloid leukemic cells to macrophages and granulocytes. Clones (D(+)) of a cultured line of myeloid leukemic cells can be induced to undergo normal differentiation to mature macrophages and granulocytes. There are also clones derived from the same cell line (D(-)) that could not be induced to differentiate. The carbohydrate-binding protein concanavalin A was used as a probe to study the mobility of carbohydrate-containing sites on the surface membrane of these cells. Changes in the distribution of concanavalin A binding sites on the surface membrane can be induced by concanavalin A. With the appropriate site mobility, this induction of a new distribution resulted in a concentration of concanavalin A-membrane site complexes on one pole of the cell to form a cap. D(+) and D(-) clones showed 50 and 5% of cells with caps, respectively, although both types of cells bound a similar number of concanavalin A molecules. Treatment of cells with trypsin increased cap formation from 5 to 40% in D(-) cells, but did not change the percentage of cells with caps in D(+) cells. The results show a difference in the mobility of concanavalin A binding sites in these two types of cells and suggest a difference in the fluid state of these carbohydrate-containing structures on the surface membrane. It is suggested that a gain of the ability of myeloid leukemic cells to undergo normal differentiation is associated with an increase in the fluidity of structures on the surface membrane where the concanavalin A sites are located. Differences in fluidity of specific membrane sites may also explain differences in the response of cells to other differentiation-inducing stimuli.


The life and work of the dental surgeon and oral surgeon in the Peoples Republic of China.


Experience with the Levant frame for cranio-maxillary fixation.


Odontogenic myxoma of the maxilla; a case report and some interesting histological findings.


An assessment of postgraduate education in oral surgery for the general dental practitioner.


Antimicrobial substances in saliva: sulphamethoxazole and trimethoprim (co-trimoxazole).


Procainamide-induced systemic lupus erythematosus.


In vitro induction of antibody formation with immunogenic RNA.


Immunogenicity of mouse spleen RNA.

Immunogenicity of mouse spleen RNA.


In vivo association in rabbits of RNA with D- and L-polypeptides.


Discussion paper: persistence and size of homologous RNA incorporated by rabbit spleen cells.


Problems in the evaluation of RNAs directing the synthesis of specific antibody.


Transfer of cell-mediated immunity by immune RNA assessed by migration-inhibition.


Immunogenic RNA in cell-mediated immunity. Introduction: transfer factor (s) and delayed-type hypersensitivity.


Transfer of transplantation immunity with RNA.


Discussion paper: biological significance of macrophage RNA.


RNA extracts in tumor immunity. Induction of anti-cancer immunity with RNA.


The adjuvant effect of natural and artificial RNA-polynucleotides in the immune response in culture.


Discussion paper: subcellular transfer of activity.


Immunogenic RNA in the immunotherapy of cancer: the transfer of antitumor cytotoxic activity and tuberculin sensitivity to human lymphocytes using xenogeneic ribonucleic acid.


Experimental models and the role of RNA in immunotherapy of leukemia.


The transfer of tumor immunity with syngeneic RNA.


The role of RNA in cell-mediated immunity.


Summary of workshop on RNA transfer of immunity.


Effect of RNA-rich liver extracts on the response of mice to a syngeneic tumor graft.


RNA in the immune response. Introductory remarks.


The role of RNA in the immunological deficiency of plasmacytoma.


The involvement of reiterative DNA in the antibody response.


The role of RNA in antibody formation. Historical perspectives.


Hepatic phosphorylase deficiency: a biochemical study.


Surgery in management of patients with leukaemia. Though leukaemia is not a "surgical" disease, the need for surgery in patients with leukaemia is increasing. Acute surgical problems in such patients present diagnostic difficulties, and accepted surgical principles do not necessarily apply in patients with very abnormal haematological and immunological features. The improved prognosis in some types of leukaemia means that elective surgical procedures, which formerly would not have been considered, may now be applicable just as they would be in patients with non-malignant conditions.Recent advances in the management of the leukaemias include several surgical procedures-for example, to facilitate intravenous or intrathecal therapy. Splenectomy is of value in chronic lymphocytic leukaemia when the correct indications are present, while early elective splenectomy, when no classical indications are present, may have a useful role in the management of patients with chronic granulocytic leukaemia.


Acute lymphatic leukemia in a boa constrictor.


Effects of the antineoplastic alkaloid acronycine on nucleoside uptake and incorporation into nucleic acids by cultured L5178Y cells.


Stimulation of oligodeoxyribonucleotide synthesis by cytoplasmic factors in the isolated nuclei of leukemic cells.


An in vitro evaluation of the chemotherapeutic potency of phenylalanine ammonia-lyase.


Wear of fluorapatite single crystals: IV. Influence of sliding direction on frictional behavior and surface failure.


Effects of estrogens on submaxillary glands in mice.


Reflex responses of the masseter and temporal muscles in man.


Wear of fluorapatite single crystals. VI. Influence of multiple-pass sliding on surface failure.


Effects of changing body position on dental occlusion.


Wear of fluorapatite single crystals. V. Influence of environment on frictional behavior and surface failure.


Effect of zinc oxide-eugenol cement containing monofluorophosphate on fluoride uptake and solubility of enamel.


Lipid and calculus matrix calcification in vitro.


Mechanism for the retardation of the acid dissolution rate of hydroxapatite by strontium.


Encephalopathy following CNS prophylaxis in childhood lymphoblastic leukemia.


An improved medium for differentiating acidogenic bacteria.


Induced calcification in the subdermal tissues of the rat.


Wear characteristics of burs.

Wear characteristics of burs.


A histological study of the periapical tissues of incompletely formed pulpless teeth filled with calcium hydroxide.


Effects of cations on the activation of salivary amylase: I. Na+, K+, Ca2+ and Mg+.


The significance of urinary bladder displacement noted on whole-body 18F bone scintigraphy.


Roentgen diagnosis of female stress urinary incontinence. Roentgenological and clinical study.


Comparison of the effects of parasympathetic denervation and administration of testosterone propionate on submaxillary glands of male mice.


Radiation treatment of carcinoma of the uterine cervix.


Video recordings of palaeopathology.

Video recordings of palaeopathology.


Factors affecting the mechanical properties of bone.


Effect of carbonates on the acid solubility rate of enamel.


Chemical protection of mammalian oral mucosa exposed to daily local radiation.


Impact of the liberalized abortion law in New York City on deaths associated with pregnancy: a two-year experience.


Preferential induction of rough variants in Streptococcus mutans by ethidium bromide.


Mast cells and basophils in the mandibular alveolar connective tissue and bone marrow of germfree albino rats.


Pathophysiology of the neurogenic bladder.


Letter: Leukemic infiltration of the testes in acute myelocytic leukemia during bone marrow remission.


Molecular genetics and developmental biology.


Reference table for human parotid saliva collected at varying levels of exogenous stimulation.


Editorial: Treatment of childhood leukemia.


Particle-bound phytochrome from maize and pumpkin.


Structure at 4.5 A resolution of a phosphorylcholine-binding fab.


President's page.

President's page.


Clinicopathological conference: Seventeen year old girl with abdominal pain of three weeks duration.


Multiplicity of ribosomal RNA genes in Vicia species with different nuclear DNA contents.


Amphetamine-induced disruption of temporal discrimination by response disinhibition.


Effect of fluoride supplementation of a citrus beverage on the erosion rate of molars of hamsters.


Structural organization of the transcription of ribosomal DNA in oocytes of the house cricket.


Improved tarnish resistance in dental amalgams.


Sports medicine symposium, weight cutting and wrestling.


A method for lysing Bacterionema matruchotii.


Preparation of cleared specimens for pulp cavity studies.


Crutanium: a partial denture alloy containing titanium.


Lead determination in human parotid saliva.


Incidence of Streptococcus mutans in root canals.


A comparative trial of remission induction (by cytosine arabinoside, or C.A.R. and thioguanine, or C.A.R. and daunorubicine) and maintenance therapy (by C.A.R. or methylgag) in acute myeloid leukaemia.


Adherence of bacteria to oral epithelium in vivo: electron microscopic observations.


Crystalline interface formed by polyacrylic acid and tooth enamel.


Phosphoric acid conditioning of teeth for pit and fissure sealants.


Reticulo-endothelial and blood-pool scanning in the diagnosis and differentiation of space occupying lesions of the liver.


Phytochemical investigation of Virola peruviana, a new hallucinogenic plant.


An assessment of the place of microvascular repair in traumatic amputations of the upper limbs.


Suprapubic bladder drainage in gynaecological surgery.


Retromammary tumours of the pectoralis major muscle.


Acute cholecystitis: the question of early of late operation.


Paravesical granuloma presenting as a late complication of herniorrhaphy.


Supraclavicular catheterization of the subclavian vein.


Haemophilia: the natural history of a destructive haematoma over a period of 50 years.


Compressed air injury of the colon: a report of two cases.


Aneurysmal bone cyst of the patella.

Aneurysmal bone cyst of the patella.


Delayed ulnar nerve palsy in colles' fracture: a case report.


Appendicitis causing acute intestinal obstruction with strangulation.


Carcinoma associated with renal tuberculosis: a report of two cases, with a brief review of the literature.


A sliding bracket shoe-piece: a new device to assist brace loading.


Intramedullary spinal tumours: a report of three cases, with special reference to early symptoms.


Propagation of epileptiform activity in the brain examined by frequency-wavenumber analysis.


Pullthrought resection of the rectum with vaginocystoplasty for repair of a rectovesicovaginal fistula.


Liver scanning for the detection of metastases following colo-rectal cancer surgery.


Editorial: JDE: flexibility.

Editorial: JDE: flexibility.


Skills analysis of cavity preparations: class 1 in mandibular right first molar.


Preventive dentistry: proposal for definition of terms.


Experimental utilization of auxiliaries in removable prosthodontics.


Honor code for dental students: follow-up study.


Mini-course in dental literature utilization.


Operative dentistry's responsibility to the profession: achieving, determining, and maintaining competence.


Introducing plaque control in the dental school.


Evaluation of the student in clinical periodontics.


Role of dental school pharmacy in education and patient service.


Games, a strategy for learning.

Games, a strategy for learning.


Inhibition of DNA and RNA synthesis, but minimally of protein synthesis, in human leukemia cells by a serine antimetabolite.


Acute paraplegia as the initial symptom of acute leukemia. Case report.


Large bilateral traumatic bone cysts of the mandible: report of case.


Medical evaluation by the oral surgeon.

Medical evaluation by the oral surgeon.


Editorial. Present and future changes in the ABOS.


Lateral sensory inhibition: a possible mechanism for paradoxes in the localization of dental pain.


Case 3. 2. Eosinophilic granuloma of the mandible.


Septic emboli and pulmonary abscess secondary to odontogenic infection.


Intraoral mandibular resection for osteoradionecrosis.


Nasal septum perforation following total maxillary osteotomy: report of case.


Papillary cystadenoma of the lower lip: report of case.


Auditory canal drainage from a masticator space cellulitis.


Benign chondroma of the palate: report of case.


Rhinolith.

Rhinolith.


Radiographic visulization of the bone wall between cysts and the antrum.


Pericarditis secondary to tooth extraction in a patient with myeloid metaplasia: report of case.


Cytogenetic analysis as a diagnostic aid in leukemia.


Induction of leukemia by 131-I treatment of thyroid carcinoma. The records of 194 patients with thyroid carcinoma treated with (131)I, representing all cases thus treated in Denmark from 1948 to 1972, were reviewed. Two cases of myeloid leukaemia were found compared with 0·097 expected cases of non-lymphocytic leukaemia (0·05 > P > 0·01). In 5 series of (131)I treated thyroid carcinomata, 10 cases of myeloid leukaemia occurred in a total of 487 patients, corresponding to a frequency of leukaemia of about 2%. These findings appear to show that (131)I treatment of thyroid carcinoma is associated with a certain risk of development of leukaemia. This risk must be considered when treatment of localized thyroid carcinoma is planned.


Portal diversion for the treatment of glycogen storage disease in humans.


The effects of shift work on worker health.


Effects of two unilateral cordotomies on the motility of the lower limbs.


Treatment of the drug abuser.

Treatment of the drug abuser.


Formaldehyde: how much of a hazard?

Formaldehyde: how much of a hazard?


Hepatic nuclear glycogenosis in the cow: a light- and electron-microscope study.


Function of steep cusps in mastication with complete dentures.


Physiologically generated occlusion.

Physiologically generated occlusion.


Artificial occlusal surfaces.

Artificial occlusal surfaces.


Tooth mobility changes in response to occlusal interferences.


Evaluating crown contours of a wax pattern.


Additional retention for acrylic veneer crowns.


Simplified waxing of veneer crowns to receive acrylic resin.


Reproduction of tilt of a cast on a surveyor.


A permanent-base denture technique using fluid resin.


Sources of confusion in the study of occlusion.


Preventive prosthodontics.

Preventive prosthodontics.


Precision attachments: uses and abuses.

Precision attachments: uses and abuses.


Parallel pins in gold restorations.

Parallel pins in gold restorations.


Prosthodontic approach to edentulous patients.


Equilibration of natural dentition.

Equilibration of natural dentition.


An essential question of occlusion.

An essential question of occlusion.


Personalized restorations vs. plates.

Personalized restorations vs. plates.


Occlusal concepts in complete dentures.

Occlusal concepts in complete dentures.


The relining of complete dentures.

The relining of complete dentures.


Problems in oral re-rehabilitation.

Problems in oral re-rehabilitation.


Variations in registering interarch transfers in removable partial denture construction.


Complete denture construction in the United Kingdom.


Functional positions of occlusion.

Functional positions of occlusion.


Modern oral preventive techniques.

Modern oral preventive techniques.


A systematic approach to restorative procedures in a physiologic occlusion.


Esthetic factors in anterior tooth restoration.


Comparison of condylar position in centric relation and in centric occlusion in dentulous subjects.


Temporomandibular joint physiology related to occlusal reconstruction.


The centric position: replacement and character.


Influence of neuromuscular conditioning on centric relation registrations.


Centric relation recording techniques: a comparative investigation.


Iatrogenic factors in periodontal prostheses.


The present status of endosteal oral implants.


Terminal dentistry and the atrophied mandible.


Subperiosteal and endosteal blade implants.


The endosteal blade-vent.

The endosteal blade-vent.


Maxillofacial prosthetics: a phase of prosthodontics.


Maxillofacial prosthetics--a controversial subspecialty.


The function of maxillofacial prosthetics of congenital oral facial defects.


Functional and cosmetic restoration of a massive orofacial defect.


Maxillofacial prosthetics program at Indiana University School of Dentistry and Medical Center.


Prosthetic reconstruction following maxillectomy.


Prosthodontics and the single-concept film.


Occlusion: point of significance in planning restorative procedures.


Evaluation of periodontal prostheses.

Evaluation of periodontal prostheses.


Cybernetic occlusion.

Cybernetic occlusion.


Instrument selection and mandibular movement recording procedures.


Expansion and contraction of dental materials.


Remounting procedure for completion of full-mouth rehabilitation.


Complete restorative dentistry.

Complete restorative dentistry.


New dental cements and fixed prosthodontics.


Immediate interim tooth-supported complete dentures.


Biologic approach to complete dentures.

Biologic approach to complete dentures.


Occlusal adjustment.

Occlusal adjustment.


Neuromuscular control of mandibular movements.


Evidence that enzyme polymorphisms are selectively neutral.


Induction of interferon by "Sepharose"-bound poly(I)-poly(C).


Antibody dependent cellular immunity in newborn mice.


Hyperactivity of remaining dopaminergic neurones after partial destruction of the nigro-striatal dopaminergic system in the rat.


Serum uric acid levels in New Zealanders.


Development of acute leukaemia in a patient with lymphosarcoma.


Urinary excretion of cadmium, zinc and copper in normotensive and hypertensive women.


Fixed by agreement.

Fixed by agreement.


Editorial: Sarcoidosis.

Editorial: Sarcoidosis.


Editorial: Antihypertensive therapy and established cerebrovascular disease.


Crural venous obstruction or incompetence. A comparative study of the diagnostic value of clinical examination, venography and plethysmography.


Psychiatric services, planning and organisation and family therapy.


Presidential address.

Presidential address.


Luteal phase defects: recognition and management.


Free standing abortion clinics: a new phenomenon.


Cytochemistry and ultrastructure of pathologic granulation in myelogenous leukemia.


Doctors and social responsibility.

Doctors and social responsibility.


Letter: Genetic councelling in children with acute leukemia cured by intensive chemotherapy.


Doctors and dependency.

Doctors and dependency.


Attitudes of Nova Scotia physicians to child abuse.


Care and feeding of the foetus. Some social implications.


Do they really pay you to play?

Do they really pay you to play?


A model for the routine evaluation of a hospital program.


The impact of diabetic day care centres on hospital utilization.


Development of secondary referral centres in Nova Scotia. Viewpoint of an internist.


Viewpoint of a rural general practitioner.


Nuclear medicine in Nova Scotia.

Nuclear medicine in Nova Scotia.


Diet and diseases of the gastrointestinal tract.


Theory of muscular contraction extended to groups of actin sites. It was shown in an earlier paper how to connect, in principle, the biochemical states of a cross-bridge with the mechanics of muscular contraction, by the methods of statistical mechanics. The treatment applies to cross-bridges that are able to interact with only one actin site at a time. The present paper shows that it is a straightforward matter to extend the theory to groups of actin sites (three, five, etc.), say 55 A apart, as suggested by the work of Moore, H. E. Huxley, and DeRosier. The possibility of the cross-bridge attachment slipping between sites is included. This provides an alternative molecular interpretation of the model introduced by A. F. Huxley and Simmons. A second possible interpretation is also suggested: their discrete stable angles correspond to different biochemical (attached) states. The Huxley-Simmons analysis of an example is rederived and extended somewhat (x averaging), from the point of view of the present theory. Their qualitative conclusions are left unchanged by the x averaging, but significant quantitative effects are possible. Possible consequences of fast slipping in isotonic contraction are discussed in a preliminary way.


Regional localization of loci for human PGM and 6PGD on human chromosome one by use of hybrids of Chinese hamster-human somatic cells. The human gene loci phosphoglucomutase(1) (PGM(1), EC 2.7.5.1) and 6-phosphogluconate dehydrogenase (6PGD, EC 1.1.1.43), which are located on human chromosome one, have been assigned to a specific region of the short arm of that chromosome, by use of a hybrid cell line derived from a Chinese hamster cell line deficient in hypoxanthine phosphoribosyl transferase and a strain of human diploid fibroblasts. Cytogenetic analysis of a hybrid clone maintained for about 50 generations in vitro revealed two populations of cells, the first containing a human chromosome one with a break point at band 1p33, such that about 25% of the short arm of this chromosome was deleted. The second cell population contained a normal chromosome one. Biochemical analysis of subclones derived by cloning this mixed population revealed two phenotypic classes, one of which expressed all three chromosome-one markers, PGM(1), 6PGD, and peptidase C (Pep C), while the other expressed only Pep C. Cytogenetic analysis showed that the subclones expressing all three markers carried the normal human chromosome one, while those expressing only Pep C carried the deleted chromosome. These data indicate that the human gene loci PGM(1) and 6PGD are located on the short arm of chromosome one distal to the break point, while Pep C lies elsewhere on the chromosome.


A family of three related satellite DNAs in Drosophila virilis. Isolated single DNA strands of satellites II or III of Drosophila virilis form hybrid duplexes with the complementary single strands of satellite I. The hybrids denature at a higher temperature than controls, renature rapidly, and form bands of hybrid density in neutral CsCl. Isolated single strands of satellite II do not form clear-cut duplexes with the complementary strands of satellite III. Mixtures of satellites II and III denature at the same temperature as controls, and do not form bands in neutral CsCl. Therefore, satellite I-II and I-III complexes are extensively base paired, while satellite II-III complexes are minimally base paired. These experiments demonstrate partial homology among the three satellites, and suggest an evolutionary relationship among them. They also suggest that satellites and other repeated sequences from related species, which do not form hybrids in vitro, could have a common evolutionary origin, but have accumulated enough base substitutions to lose interspecific homology demonstrable by hybrid duplex formation.


Enhancement by interferon of the expression of surface antigens on murine leukemia L 1210 cells. Preparations of mouse interferon enhanced the expression of surface antigens of murine leukemia L 1210 cells, as determined by their alloantibody-absorbing capacity. The factor responsible for the enhancement of surface antigen expression could not be dissociated from the antiviral activity of interferon by standard physicochemical means. Likewise, interferon did not increase the antibody-absorbing capacity of an interferon-resistant subline of L 1210 cells. We conclude that interferon treatment of L 1210 cells is accompanied by modifications of the cell surface.


Regulation of RNA synthesis in fibroblasts during transition from resting to growing state. Addition of serum, containing fibroblast growth factors, to a culture of resting 3T6 cells stimulates a transition to the growing state. Studies of ghost monolayers prepared with the aid of detergent at intervals after stimulation showed an increase in the rate of ribosomal RNA synthesis within 10 min. The rate continued to increase for many hours and reached a level 2.5- to 3.5-fold higher by the time DNA synthesis began. The increasing rate of ribosomal RNA synthesis appeared independent of an increase in the number of ribosomal genes, since it was not affected by prevention of DNA synthesis with cytosine arabinoside. In contrast to ribosomal RNA, the overall rate of transscription of heterogeneous nuclear RNA was not directly affected by serum growth factors and does not appear to be regulated during the transition from resting to growing state. It seems, instead, to be fixed in relation to the amount of template, for it increases proportionally to DNA content.


Secondary structure maps of RNA: processing of HeLa ribosomal RNA. Ribosomal RNA precursors and mature 28S ribosomal RNA from HeLa cells display a highly reproducible secondary structure of hairpin loops after they are spread and examined in an electron microscope. This structure was used to map the linear arrangement of these molecules. Partial digestion with 3'-exonuclease from ascites cell nuclei established that the 28S RNA region is located at the 5'-end of the 45S precursor. A spacer sequence follows the 28S region. This is followed by the 18S RNA region, and by another spacer sequence at the 3'-end. The 41S RNA contains both 18S and 28S regions. The 32S RNA contains only 28S RNA plus spacer, and the 20S RNA contains only 18S RNA plus spacer. Several minor nucleolar RNA components were also mapped with respect to the 45S RNA. These experiments lead to a more detailed processing pathway for HeLa ribosomal RNA which is in good agreement with earlier work.


Resolution of DL-tryptophan by affinity chromatography on bovine-serum albumin-agarose columns. Bovine-serum albumin, known to have antipodal specificity in the binding of tryptophan, was selected as the affinity chromatographic matrix for the attempted chromatographic resolution of DL-tryptophan. Complete resolution was accomplished when Dl-tryptophan was chromatographed on bovine-serum albuminsuccinoylaminoethyl-Sepharose.


A new concept for energy coupling in oxidative phosphorylation based on a molecular explanation of the oxygen exchange reactions. The P(i) right arrow over left arrow HOH exchange reaction of oxidative phosphorylation is considerably less sensitive to uncouplers than the P(i) right arrow over left arrow ATP and ATP right arrow over left arrow HOH exchanges. The uncoupler-insensitive P(i) right arrow over left arrow HOH exchange is inhibited by oligomycin. These results and other considerations suggest that the relatively rapid and uncoupler-insensitive P(i) right arrow over left arrow HOH exchange results from a rapid, reversible hydrolysis of a tightly but noncovalently bound ATP at a catalytic site for oxidative phosphorylation, concomitant with interchange of medium and bound P(i). Such tightly bound ATP has been demonstrated in submitochondrial particles in the presence of uncouplers, P(i), and ADP, by rapid labeling from (32)P(i) under essentially steady-state phosphorylation conditions. These results lead to the working hypothesis that in oxidative phosphorylation energy from electron transport causes release of preformed ATP from the catalytic site. This release could logically involve energy-requiring protein conformational change.


Functions of a new photoreceptor membrane. The purple membrane of Halobacterium halobium contains only one protein, bacteriorhodopsin, which closely resembles the visual pigments of animals. Light flashes cause a rapid transient shift of its absorption maximum from 560 to 415 nm. This shift is accompanied by release and uptake of protons. Respiring cells acidify the medium in the dark; if they contain purple membrane their O(2) consumption is reduced in the light. Starved or anaerobic cells containing purple membrane, in the absence of any apparent source of energy, generate and maintain a proton gradient across the cell membrane as long as they are exposed to light. We postulate that the light-generated proton gradient arises from a vectorial release and uptake of protons by bacteriorhodopsin, which is suitably oriented in the cell membrane and under continuous illumination oscillates rapidly between the long- and short-wavelength form. Preliminary results indicate that the gradient in H. halobium plays the central role in energy coupling attributed to such electrochemical gradients by Mitchell's chemiosmotic theory.


Multiple common properties of human beta2-microglobulin and the common portion fragment derived from HL-A antigen molecules. 11,000-Dalton common portion fragments derived from HL-A antigen molecules were isolated and found to have a significantly high homology to beta(2)-microglobulin in amino-acid composition. Common portion fragments are also very similar to beta(2)-microglobulin with respect to molecular size, charge, and distribution in tissues. Moreover, both are found in the spent culture media of human cell lines and in human plasma and urine. Thus it appears that beta(2)-microglobulin may well be the same substance as the common portion fragment of HL-A antigen molecules.


Nuclear magnetic resonance study of hydrogen-bonded ring protons in Watson-Crick base pairs. We have studied the low-field proton magnetic resonance spectrum of the double-helical complex of d(A-A-C-A-A) with d(T-T-G-T-T). Well-resolved resonances for the thymidine N(3) and the guanosine N(1) protons are seen at 1 degrees C, which broaden by 4 degrees C and disappear above 9 degrees C. The actual T(m) of the complex was found to be 28 degrees C by measurement of UV-absorbance change. There are several factors that could limit the observed exchange broadening of the resonance lines; the model that best fits the data is one in which the rate of double-helix dissociation limits the rate of exchange of the H-bonding protons with water.


Maternal histone messenger RNA: detection by molecular hybridization. A competition-hybridization assay has been developed for the majority of sequences present in embryonically synthesized histone messenger RNA. The assay permits the first direct demonstration of specific "maternal" messengers in unfertilized sea-urchin eggs. The molecular size of histone messenger RNA stored in the egg appears to be the same as that of histone messenger RNA synthesized by the embryo. Maternal histone messenger RNA is found in the soluble phase of egg homogenates, in the form of ribonucleoprotein particles, unassociated with ribosomes.


Substrate binding to an active creatine kinase with a thiol-bound mercurinitrophenol chromophoric probe. A mol of creatine kinase (EC 2.7.3.2), an enzyme with two identical subunits can be titrated very rapidly and stoichiometrically with 2 mol of 2-chloromercuri-4-nitrophenol, an environmentally sensitive chromophoric probe specific for thiols. Chemical modification does not inactivate the enzyme nor can the reaction be prevented by a dead-end quaternary complex of enzyme, magnesium, ADP, and creatine with added nitrate. Creatine kinase modified with the chromophoric reagent can be further reacted with 200-fold molar excess of iodoacetamide, resulting in loss of enzymatic activity but without release of bound mercurinitrophenol. The tightly bound chromophore has spectral properties different from free unbound mercurinitrophenol. Only addition of MgADP, or MgATP and creatine, or nitrate, to the modified though active enzyme causes further changes in the visible spectrum of the bound nitrophenol. Addition of nitrate, Mg, ADP, and creatine to the enzyme with bound reporter group, in the order indicated, caused spectral changes; each addition gave a different spectrum.


Identification of chick chromosomes in cell hybrids formed between chick erythrocytes and adenine-requiring mutants of Chinese hamster cells. When chick erythrocytes were fused with adenine-requiring mutants of Chinese hamster cells of two different complementation classes and grown in adenine-free medium for 1 week or longer, mitotic cells were observed containing both chick and Chinese hamster metaphase chromosomes within a single cell. Stable hybrid clones were subsequently isolated that possessed, in addition to a complete Chinese hamster genome, a single specific chick chromosome. Hybrids resulting from fusions involving different adenine mutants retained different, identifiable chick chromosomes. Thus, the two chick genes for the endogenous synthesis of adenine were assigned to the respective chick chromosomes.


Regulation of acetylcholine receptors in relation to acetylcholinesterase in neuroblastoma cells. Purified alpha-toxin from Naja nigricollis snake venom labeled by [(3)H]acetylation binds specifically to the acetylcholine receptors of mouse neuroblastoma cells. Toxin binding was inhibited by inhibitors for nicotinic and muscarinic acetylcholine receptors. Clones of neuroblastoma cells were selected for low acetylcholinesterase (EC 3.1.1.7) activity with antibodies against this enzyme. Selection for an 80-fold decrease in acetylcholinesterase activity was not associated with any decrease in the number of acetylcholine receptors (3.4 x 10(7) per cell). Removal or inactivation of 80% of the acetylcholine receptors by proteolytic enzymes or by compounds that block sulfhydryl groups did not change the activity of acetylcholinesterase on the cell surface. In addition to these results on the separation between acetylcholine receptors and acetylcholinesterase, a common regulation was found in that both the number of acetylcholine receptors and the activity of acetylcholinesterase were increased 5- to 10-fold when the cells stopped to multiply or were induced to differentiate by dibutyryl-cyclic AMP. It is suggested that there are different genes for the acetylcholine receptor and acetylcholinesterase, and that both are regulated during growth and differentiation by a common regulatory gene.


The amplification of ribosomal RNA genes involves a rolling circle intermediate. During the development of Xenopus oocytes there is a special DNA synthesis that leads to a thousandfold amplification of the genes that code for ribosomal RNA. We have used the electron microscope to study this process. Our primary observation is the presence of ribosomal DNA in rolling-circle intermediates at the time of amplification. We believe that these intermediates are involved in the amplification process, and as such offer the first example of the involvement of a rolling circle in the replication of eukaryotic DNA.


Interaction of nerve growth factor with surface membranes: biological competence of insolubilized nerve growth factor. Nerve growth factor was insolubilized by covalent attachment to Sepharose beads. Nerve growth factor-Sepharose was biologically active in both the neurite outgrowth assay for nerve growth factor and in preserving responsive neurons in vitro. Modification of the bioassay to detect solubilized activity of nerve growth factor and histological examination of ganglia treated with nerve growth factor-Sepharose revealed that nerve growth factor-Sepharose prepared by reaction in 6 M guanidine hydrochloride released negligible amounts of solubilized nerve growth factor activity. These observations extend the previously noted correlations on the structure and function of nerve growth factor and insulin to include the primary action of these two proteins. Thus nerve growth factor, like insulin, appears to express its biological activity by first binding to a receptor on the surface membrane of responsive cells.


Structual comparison of ectopic and normal placental alkaline phosphatase. An alkaline phosphatase immunochemically similar to placental alkaline phosphatase (EC 3.1.3.1) was purified from liver metastases of a giant-cell carcinoma of the lung. Some properties of its physical and chemical structure were determined and compared to those of purified placental alkaline phosphatase. The purified tumor phosphatase and the placental phosphatase were similar with regard to the following properties: (1) NH(2)-terminal sequence, (2) peptide map, (3) subunit molecular weight, and (4) isoelectric point. The physical properties and NH(2)-terminal sequence of alkaline phosphatase isoenzyme of liver differed from the placental and the tumor enzyme. The data from the present study strongly support the hypothesis that the tumor and the placental alkaline phosphatases are products of the same gene.


A theory of the epigenesis of neuronal networks by selective stabilization of synapses. A formalism is introduced to represent the connective organization of an evolving neuronal network and the effects of environment on this organization by stabilization or degeneration of labile synapses associated with functioning. Learning, or the acquisition of an associative property, is related to a characteristic variability of the connective organization: the interaction of the environment with the genetic program is printed as a particular pattern of such organization through neuronal functioning. An application of the theory to the development of the neuromuscular junction is proposed and the basic selective aspect of learning emphasized.


Effects of 5-bromo-2' -deoxyuridine on production of globin messenger RNA in dimethyl sulfoxide-stimulated Friend leukemia cells. Friend leukemia cells grown in the presence of dimethyl sulfoxide show enhanced erythroid differentiation and hemoglobin synthesis. The effects of dimethyl sulfoxide stimulation are inhibited by BrdU. To determine the effect of BrdU on the amount of globin mRNA present in cells treated and not treated with dimethyl sulfoxide, molecular hybridization between total cell RNA and [(3)H]DNA complementary to mouse globin mRNA was used. Cells treated with BrdU and dimethyl sulfoxide had 70% less globin mRNA than cells treated with dimethyl sulfoxide alone. The size and base sequence of the residual globin mRNA in the cultures treated with BrdU and dimethyl sulfoxide were unaltered. Cells treated with BrdU alone contained slightly more globin mRNA than did the untreated controls, suggesting that BrdU may have a dual effect in transcription of messenger RNA.


Collagen synthesis: a disulfide-linked collagen precursor in chick bone. Chick calvaria labeled in vitro contain a triple-stranded collagen precursor that contains two pro alpha1 and one pro alpha2 chains. All chains contain cystine and are linked by S-S bonds. Ultracentrifugal analysis was used to measure the relative size of the disulfide-linked units. It is proposed that the S-S links help to determine the correct alignment of the triple-stranded structure.


The occurrence of isolated dysplastic and carcinoma in situ type cells in cervical smears from patients with dysplasia and carcinoma in situ: significance to prescreening using image processing techniques.


Electron microscopic studies of cells in pleural and peritoneal effusions.


"The mini-conor," evaluation as a biopsy technique in patients with abnormal cervical cytology.


Prediction of visual effects from the warpage of spectacle lenses.


Base line fracture resistance studies of tempered and non-tempered glass ophthalmic lenses.


Personality traits and failure in wearing contact lenses.


The influence of bias upon retinoscopy.

The influence of bias upon retinoscopy.


Letter: The relation between basketball ability and visual acuity in the unicorn.


Case report: underwater contact lens correction.


The differential diagnostic significance of bone-marrow biopsy in haemoblastoses.


Letter: Pyoderma gangrenosum and leukemia.


Influences of hepatic portal receptors on hypothalamic feeding and satiety centers.


Osteosarcoma of the larynx.

Osteosarcoma of the larynx.


A concept and classification of centers of rotation and extraoral force systems.


The diagnosis of breast lesions from cytologic material.


Variation in the secular changes in the teeth and dental arches.


Periodontal condition in orthodontically treated and untreated individuals. I. Loss of attachment, gingival pocket depth and clinical crown height.


Maxillary growth velocity and variation in three dimensions during treatment of class 3 malocclusion.


A technique for indirect archwire construction.


Biracial study of the maxillary midline diastema.


Autoradiographic method for an expanded assessment of colonic cytology.


X chromosomal anomalies in the newborn.

X chromosomal anomalies in the newborn.


A clarification of the surgical goals in cleft palate speech and the introduction of the lateral port control (l.p.c.) pharyngeal flap.


Parents working with parents: the cleft palate program.


Your security.

Your security.


The association of fetal wastage with facial cleft conditions.


The Emerson ventilator, again.

The Emerson ventilator, again.


A computer program for articulation tests.


The intellectual function of cleft palate children compared on the basis of cleft type and sex.


Transnasal aspiration biopsy of central skull base destructions.


The influence of palatoplasty and cleft length on facial development.


Cost benefit analysis: application to dental services.


Cervical cytology: descriptive statistics for nuclei of normal and atypical cell types.


Cytodiagnosis of HCG-secreting choriocarcinoma of the stomach. Report of a case.


Editorial: A philosophy for technology.

Editorial: A philosophy for technology.


The precursors of endometrial cancer: a study of their cellular manifestations.


Diagnosis and treatment of cervicofacial actinomycosis.


The career commitment of female dental graduates.


Dental hygiene programs: administrators and faculty.


Women in dentistry and medicine: attitudinal survey of educational experience.


Pilot study on criteria in cavity preparation--facts or artifacts?


Widened horizons for video tape in dental education.


Routine screening for hypertension in dental practice: fundamental discipline of dental education.


Editorials: Team, team, team.

Editorials: Team, team, team.


Editorial: Women in dentistry.

Editorial: Women in dentistry.


First-year preventive dentistry curriculum at University of the Pacific.


Editorials: The temptation to advertise.


Nihilism and despair, rebirth and repair. A psychoanalytic view of the adolescent crisis.


Mesothelioma as primary tumor of the peritoneum. A case report.


Procurement of teaching patients: cooperative effort between social service agencies and dental school.


Psychotropic drugs in old age.

Psychotropic drugs in old age.


Cardiac surgical mortality for adults at the Deborah heart and lung center.


Influence of exercise and high protein diet on blood chemistry. A study of athletes.


CMDNJ notes.

CMDNJ notes.


Therapeutic drug information center.

Therapeutic drug information center.


Communicable diseases in New Jersey.

Communicable diseases in New Jersey.


Physicians seeking location in New Jersey.


Limitations of the radiograph in the diagnosis of osseous defects in periodontal diseases.


Periodontal ligament of a rat incisor tooth.


Effect of asparagine and glutamine deficiency on normal and leukemic cells.


The influence of urea peroxide gel on plaque, calculus and chronic gingival inflammation.


Oral changes in a folic acid deficient patient precipitated by anticonvulsant drug therapy.


The effect of sodium fluorescein on the mucosa of the hamster.


The imported fire ant. A newly recognized public health problem in South Carolina.


The physician and fire ants.

The physician and fire ants.


Pruritus ani: a tale of the itch.

Pruritus ani: a tale of the itch.


South Carolina maternal mortality review for 1970.


Contrast radiography of abdominal cyst from ventriculo-peritoneal shunt.


X-ray film of the month. The plump hilum--an overlooked finding of pulmonary embolism.


President's pages.

President's pages.


Analysis of behavior in a terminally ill child.


Alterations in the temporomandibular joint.


Mandibular relearning.

Mandibular relearning.


Total maxillary osteotomy for correction of fracture malunion.


Oral surgery and the patient who has had radiation therapy for head and neck cancer.


Oral pigmentation associated with bronchogenic carcinoma.


Complex odontoma in the maxillary sinus.


Pachyonychia congenita. A long-term evaluation of associated oral and dermal lesions.


Anesthetic and dental management of a child with epidermolysis bullosa dystrophica.


X-linked hypomaturation type of amelogenesis imperfecta exhibiting lyonization in affected females.


Some new findings in the basal-cell nevus syndrome.


Dental abnormalities in the tricho-dento-osseous syndrome.


Primary malignant melanoma of the oral cavity.


Granular-cell odontogenic cyst.

Granular-cell odontogenic cyst.


Biologic effects of dental materials. 2. Toxicity of anterior tooth restorative materials on HeLa cells in vitro.


Root canal morphology of mandibular first and second premolars.


A study of the radiographic appearance of osseous defects on panoramic and conventional films.


A broken needle in the pterygomandibular space. Report of a case.


Gemination and dens in dente.

Gemination and dens in dente.


An unusual periapical abscess.

An unusual periapical abscess.


Free nucleotides of normal and leukemic leukocytes.


Hygienic guide series: titanium.

Hygienic guide series: titanium.


Total body irradiation and human chromosomes. 3. Cytogenetic studies in patients with malignant hematologic diseases treated with total-body irradiation.


Muscle activity in normal and postnormal occlusion.


Evaluating tooth size in premolar-extraction cases.


Prediction of orthodontic band sizes from selected teeth.


Cinefluorographic and cephalometric study of Class I acceptable occlusion.


Rationale of three-premolar extraction: a case report.


Editorial: In which orthodontics discovers a defender.


On the use of the SNA and SNB angles in cephalometric analyses.


The use of nitrous oxide during ventilation with the open bronchoscope.


Interacting cell populations affecting granulopoietic colony formation by normal and leukemic human marrow cells.


Serial in vitro bone marrow culture in acute lymphocytic leukemia.


Serial in vitro marrow culture in acute myelocytic leukemia.


Chloroma and other myeloblastic tumors.

Chloroma and other myeloblastic tumors.


Presidential address.

Presidential address.


Valedictory address.

Valedictory address.


The occurrence of secondary caries-like lesions in vitro. The effect of a fluoride cavity liner and a cavity varnish.


The role of gnotobiotic animals in the study of dental caries.


Radiography and radiology for the dental practitioner. 3. Technique--bitewing and periapical radiography.


The introduction of preventive ancillaries.


Dental data requirements in the area health authorities.


Mercury hazards in dental practice. Assessment and control by activation analysis.


The strength of amalgam. A comparative study of test methods using various alloys.


Is this where I came in?

Is this where I came in?


Letter: Gilles de la Tourette's disease.


Scanning electron microscope study of the formation of dental plaque.


An in vivo method for the study of the microbiology of occlusal fissures.


Microbiological studies of plaque in artificial fissures implanted in human teeth.


The distribution of dental caries in ancient British populations. II. Iron Age, Romano-British and Mediaeval periods.


Orientation-dependent etchpit penetration and dissolution of fluoroapatite.


Influence of water intake on the degree of incisor fluorosis and on the incorporation of fluoride into bones and incisor teeth of mice.


Regulation of dextransucrase formation by Streptococcus sanguis.


Fluoride incorporation and apatite solubility.


Sugar and the production of bacteria in the human mouth.


The effect of topical acidulated fluoride on percentage of Streptococcus mutans and Streptococcus sanguis in interproximal plaque samples.


Some factors affecting the interaction of hydroxyapatite with sodium monofluorophosphate.


Fluoride concentrations in deciduous teeth of children using fluoride mouthrinses.


Loss of carbonate during the first stages of enamel caries.


Copper and molybdenum uptake by the hard dental tissues of the rat.


The inorganic composition of arrested carious dentine.


Fluoride concentrations in surface enamel of some teeth in Athens.


A roentgenological grid technique for the bacteriological sampling of root surface caries.


Double strand rejoining in mammalian DNA.


Isolation of nuclear pre-mRNA which codes for immunoglobulin heavy chain.


On labelling membranes.

On labelling membranes.


Proteins associated with heterogeneous nuclear RNA of newt oocytes.


Human IgG cryoglobulin with tubular crystal structure.


Effect of ouabain on the current underlying spontaneous diastolic depolarization in cardiac Purkinje fibers.


Molecular size of the tetrodotoxin binding site estimated by irradiation inactivation.


Direct determination of the electron density profile of nerve myelin.


Comparison of effects of L-dopa, amphetamine and apomorphine on firing rate of rat dopaminergic neurones.


Leukocyte kinetics in normal and leukemic subjects.


Immunologic aspects of leukemia.

Immunologic aspects of leukemia.


Neurosurgical management of intractable pain.


Dr. Jerome L. Reeves on scurvy. An interview.


Haemodynamic aspects of physical training after myocardial infarction.


Coupling reactions of nitriles and anilines during hydrogenation.


Reductive synthesis of alpha, alpha-dimethylphenethylamine.


Linear telomerization of conjugated dienes.


The catalyzed codimerization of ethylene and butadiene to vinylcyclobutane.


The selective catalytic hydrogenation of 2-(4-nitrophenyl)-4(5)-nitroimidazole.


Increasing selectivity in hydrogenation of substituted anthracenes.


Pressure processes and equipment.

Pressure processes and equipment.


Haptophilic control of hydrogenation stereochemistry.


Selective reduction of various substituted dicyclopentadienes.


Some aspects of catalytic hydrogenolysis and dehydrogenation.


The selective dehalogenation of 2,4-dichloro-5(2,4-dichlorodiphenylmethyl)pyrimidine.


Hydrogenation, racemization, and exchange of (+)-apopinene on platinum, rhodium, and palladium catalysts: a possible (1,3)-sigmatropic shift.


Selective reduction of 2'-nitroanilides.


Studies in nuclear hydrogenation.

Studies in nuclear hydrogenation.


Selective hydrogenation of a lysine intermediate.


Complete nuclear hydrogenation of 9,10-disubstituted, 9,10-bridged anthracenes and their use in the preparation of monomers.


A new high-pressure facility.

A new high-pressure facility.


Selective hydroformylation of unsaturated fatty acid esters.


Letter: Effect of polymethacrylic acid (PMAA) on tumour metastasis.


Genetic experimentation: the new ethic.

Genetic experimentation: the new ethic.


Enzymatically active intermediate in the conversion between the low and high molecular weight DNA polymerase.


Modification of precipitated morphine and methadone abstinence in mice by acetylcholine antagonists.


Studies on the regeneration of rhodopsin from all-trans-retinal in isolated rat retinae.


Auxin regulation of caulonema formation in moss protonema.


Medical engineering at the Leipzig autumn fair.


Traumatic gingival recession in infants. The result of a dummy sucking habit.


Radiography and radiology for the dental practitioner. 7. Radiology in restorative dentistry.


The displacer--an exodontic instrument.

The displacer--an exodontic instrument.


A new composite restorative material. An evaluation.


The effect of polycarboxylate containing stannous fluoride on the pulp.


The properties of a glass ionomer cement.


The sealing properties of root filling cements. Further studies.


Dilaceration of upper central and lateral incisors. A case report.


Western Samoa: delivery of dental services in an emergent nation.


The localization and retention of 32P and 45Ca within surface deposits of Streptococcus sanguis and the influence of such deposits on the release of these isotopes from enamel.


The predominant cultivable flora of carious plaque and carious dentine.


The role of carbonate in dental mineral.


The uptake of F- by hydroxyapatite at varying pH.


Dislocations and dissolution of enamel. Theoretical considerations.


The differential inorganic composition of dentine within active and arrested carious lesions.


Myofunctional therapy.

Myofunctional therapy.


Doctors, digits and disks.

Doctors, digits and disks.


Orthodontic surgery: An important part of the treatment of skeletal malocclusions. Presentation of a case.


Viral hepatitis: metabolic studies during acute and convalescent phase.


The postmastectomy lymphedematous arm.

The postmastectomy lymphedematous arm.


Community health aspects of water pollution problems.


Russian adventure.

Russian adventure.


Major aptitudes and personality characteristics of 42 dental students as evaluated through their handwriting.


Of fish and water: some thoughts for unions of health professionals.


Healthy mouths for the handicapped.

Healthy mouths for the handicapped.


University of Bridgeport football squad gets mouthguards courtesy of Fones School of Dental Hygiene.


Editorial: A dental society should protect its members.


Dentistry in Israel today.

Dentistry in Israel today.


More on the conservative treatment of follicular cysts.


Must 8 specialties equal 7 specialists + 1 traitor?


Editorial: A rebuff by HEW.

Editorial: A rebuff by HEW.


HMOs: how much oratory or help mankind out?


Orthodontics: scientific and philosophical.


The endodontic aspect of complete root amputation.


More effective methods of lacating and preparing posterior canals.


Sialolithiasis of the submaxillary duct.


Scanning electron microscopy of the enamel organ of the rat incisor.


A roentgenologic method for registering dental caries of primary molars in preschool children.


Roentgenographic assessment of overhanging margins of dental restorations. II. Significance of variations in vertical angulation of the roentgen beam.


Effect of an extra-pulpal blood clot on healing following experimental pulpotomy and capping with calcium hydroxide.


Creep in some acrylic dental resins.

Creep in some acrylic dental resins.


The attitude of Swedish dentists to post-graduate courses.


Experimental tooth hypermobility in the dog. A methodological study.


The Brown University medical curriculum.


Peer review: a comprehensive appraisal.

Peer review: a comprehensive appraisal.


Oral hypoglycemic agents: the pharmacological basis of their clinical use.


Electrocoagulation in the treatment of cancer of the rectum.


Double contrast arthrography of the knee--a report of 135 consecutive studies at Rhode Island hospital.


Providencia stuartii.

Providencia stuartii.


In-vitro sensitivity to cytostatics in chronic myeloid leukaemia.


Psychological complications of hysterectomy.


Punishment, treatment or quarantine: some problems in social defence.


The psychiatrist as administrator.

The psychiatrist as administrator.


The relevance of the neurophysiological concepts of excitation and inhibition to clinical psychiatry.


Personality disorders: some problems of nosology and classification.


The psychological health of university students as measured by a personality inventory.


Proliferation kinetics of Shay chloroleukaemia cells grown in diffusion chambers in vivo.


Childhood and adolescence on Elcho Island.


Surface immunoglobulins of leukaemic cells.


A follow-up study of former participants in a controlled oral hygiene study.


Gingival recession associated with temporary crowns.


Periodontosis in a patient with previously diagnosed sarcoidosis.


Absence of an association between Streptococcus mutans and gingivitis in caries-free naval recruits.


In vitro plaque inhibition by chlorhexidine gluconate and homologues--effect of pH and pretreatment.


In our opinion. How do you envision the practice of periodontics in the year 2000?


Professionally applied fluorides.

Professionally applied fluorides.


Life-long benefits of fluoride in drinking water.


The use of fluoride in caries prevention. Methods available to dental practitioners in the U.K.


Children with post-alveolar cleft palate. Facial growth and the development of the occlusion.


Radiography and radiology for the dental practitioner. 1. The dental x-ray set.


The endo-meter. A new method for adjusting the length of root canal instruments.


The traction hook on the modified arrowhead clasp.


Dental materials at the Laboratory of the Government Chemist.


Multiple choice question tests in dental surgery.


The senile human mandible.

The senile human mandible.


Continuous assessment of dental students.


The establishment of an experimental dental care project.


The professions supplementary to dentistry and medicine.


Teaching dental histology to dental auxiliary students. A comparison of two methods.


Postgraduate courses in oral surgery for the general practitioner.


Implications for dentistry of the Recommendations of the Royal Commission on Medical Education and Related Questions.


An assessment of attitude changes following a dental exhibition.


Fluoride in the prevention of dental caries. A tentative cost-benefit analysis.


The British Dental Association.

The British Dental Association.


Thermoluminescence dosimetry in medicine and archaeology.


Conference on partial laryngectomy for carcinoma of the larynx.


Ovarian carcinoma: pathology, staging, grading, and prognosis.


Treatment of advanced cancer of the cervix by pelvic exenteration.


Cordal carcinoma of the larynx with posterior extension: surgical treatment.


Vibration and its relation to occupational health and safety.


Management of anterior commissure carcinoma of the larynx.


Preferred preventive health care facilities: a survey among urban adolescents.


Family planning by mothers on welfare.

Family planning by mothers on welfare.


Physician manpower: the issues.

Physician manpower: the issues.


A behavioral approach to Tourette's syndrome.


Deletion of the long arm of chromosome no. 7: tentative assignment of the Kidd (Jk) locus.


Iatrogenic ileus secondary to medication.


A hospital-based methadone program. Ambulatory induction phase.


Anomalous left pulmonary artery arising from the ascending aorta.


Unexpected effects related to interactions of drugs in pediatric use.


Brain abscess.

Brain abscess.


Carcinoma of the larynx and vocal rehabilitation.


Cardiac resuscitation. A six month review.


The rehabilitation of the aged disabled.


North Carolina Medicine--looking down the road in seventy-three--seventy-four.


Planning for Career Ambulance Profession.


Treatment of chronic lymphocytic leukemia.


Leukemia and irradiation.

Leukemia and irradiation.


Fatal pulmonary infection with Mycobacterium fortuitum, Report of a Case.


Opening address: Forty-Ninth Annual Conference.


The veterinarian and the expansion of his horizons.


Toe and muscle injuries of the racing greyhound.


Reentrant cannulation of duodenum cranial to pancreatic duct in the pig.


Inherited diseases of cattle--a perspective.


Pneumonia in acute leukemia.

Pneumonia in acute leukemia.


Cytophilic antibodies in man.

Cytophilic antibodies in man.


Neurological complications of acute leukemia in adults: changing rate.


Distribution of kinase and deaminase of 1-beta-D-arabinofuranosylcytosine in tissues of man and mouse.


Replication of an avian myxovirus in primary cultures of human leukemic cells.


How automatic equipment helps process medical paperwork.


Stress is a work hazard, too.

Stress is a work hazard, too.


Management of head injury is a vital first aid measure.


Are your workers exposed to non-ionizing radiant energy?


Mini-courses for clinical teaching.

Mini-courses for clinical teaching.


Improvement in the motivation of student nurses.


Radiographic interpretation. Common diseases of elbow and shoulder joints in dogs.


Nursing image.

Nursing image.


Cultural barriers.

Cultural barriers.


Why nursing is different.

Why nursing is different.


Central nervous system infections in patients with cancer.


What can you say?

What can you say?


By nurse and machine.

By nurse and machine.


The needs of the individual.

The needs of the individual.


Churchill study tour.

Churchill study tour.


As I see it.

As I see it.


The quality of infant care.

The quality of infant care.


Successfully treated priapism in acute myeloblastic leukemia complicating Hodgkin's disease.


Influence of oestrogenic hormones on carcinogenesis and toxicity of radiostrontium.


Drugs and psychiatric readaptation.

Drugs and psychiatric readaptation.


Intermittent mandatory ventilation: a new approach to weaning patients from mechanical ventilators.


Acute leukemic cells. Qualitative and quantitative electron microscopy. Quantitative and qualitative electron microscopic studies were performed on the leukemic cells of 3 patients with stem cell leukemia, 6 patients with acute lymphoblastic leukemia, 7 patients with acute monoblastic leukemia, 3 patients with acute myeloblastic leukemia and 7 patients with acute monomyeloblastic leukemia. Significant quantitative differences were noted between some of the leukemic cells in heterochromatin:euchromatin ratios, cell size, granules per cell, amount of endoplasmic reticulum and the number of polyribosomes. Qualitative abnormalities which were found in some cells of all the leukemic groups were observed. These abnormalities included nuclear pockets, deep nuclear indentations (usually not the stem cell), nucleosomes, dilated perinuclear spaces, centrioles located in nuclear pockets (not in the stem cell or lymphoblast), accumulation of microfibrils (greatest in the monoblast and myeloblast), disrupted mitochondria with virus-like particles (none in stem cells), smaller granules and mitochondrial DNA, glycogen, and myelin whorls. The presence of the smaller granules in disrupted mitochondria and the resulting clear areas is probably related to the deranged carbohydrate metabolism of these cells. The presence of virus-like particles within mitochondria may be extremely important, but requires much more investigation. Some of the above findings may offer clues to further investigation of the acute leukemic cell.


A clinical experiment with sulpiride.

A clinical experiment with sulpiride.


Patterns of acculturation in new immigrants.


Changes in mental functioning accompanying anti-psychotic drug therapy.


Neurosis and marriage--a phenomenological analysis of disturbed married couples.


The child of two survivors. A report of an unsuccessful therapy.


Psyche-trauma-psyche: surgeons' observations of psychiatric conditions in trauma patients.


Electroencephalographic changes in schizophrenic patients.


The potential of antibiotics as caries-control agents.


Prospects for prevention of dental caries: dietary factors.


Malocclusion: new directions for research and therapy.


Plaque control in periodontal disease.

Plaque control in periodontal disease.


Salmonella javiana infection in an infant associated with a marsupial, the quokka, Setonix brachyurus, in Western Australia. Salmonella javiana, a serotype rarely isolated in Australia, has been recovered from the faeces of a 14-month-old infant with symptoms of enteritis.The child had been closely associated with a marsupial species, the quokka, during a vacation on Rottnest Island in Western Australia, and S. javiana was isolated from faecal pellets from adult quokkas, and also from a snake collected on the island.Sampling revealed a high incidence of Salmonella infection in the quokkas. In all, 62 out of 87 animals (71%) were found to be infected, and 17 Salmonella and 3 Arizona serotypes were identified from 100 isolations comprising 92 salmonellas and 8 arizonas. Multiple infections were frequently detected and up to four serotypes were recovered from individual animals. S. javiana was isolated from four quokkas.A close parallel was observed between the serotypes isolated from quokkas and sea-gulls on the island, and abattoir effluents, lake waters, bird droppings and reptiles sampled on the adjacent mainland.The epidemiological significance of Salmonella and Arizona infections in the quokka population and their possible association with the seasonal decline in condition and numbers of animals on Rottnest Island is discussed.


Caries research: the current status.

Caries research: the current status.


Thermal sensations of secondary school children in summer. Summer thermal sensations of secondary schoolchildren in England are compared with those earlier published for winter. Heat stress, contrary to expectation, is seen to decrease during the warm months, and neutrality is found to increase by some 6 degrees F. (3.5 degrees C.) air temperature, which is more than double the increases observed for adults under similar circumstances.


Electron-microscopic study of measles virus in lymphocytes of affected children. Two different types of inclusion bodies have been found by electron-microscopy in the cytoplasm of sectioned lymphocytes from children infected with measles.The first is tubular, 18 nm. internal diameter, embedded in osmiophilic material, and is found during the incubation period. It is not thought to be specific for measles, and is probably identical with structures recently reported in HEp-2 cell lines and tumour tissue.The second type is less obviously tubular and morphologically resembles measles nucleocapsid. This was found only after the rash had appeared.


Salmonella dublin abortion in cattle. I. Observations on the serum agglutination test. The somatic and flagellar serum agglutinin titre were determined in paired samples obtained from seventy-seven cases of bovine abortion associated with Salmonella dublin infection. The cases could be divided into four serological groups with an active infection being demonstrated in most cases. The serum agglutination test was shown to be a relatively specific diagnostic test but was of more limited value in the retrospective identification of convalescent cases.


Antigenic analysis of prototype influenza A (H3N2) strains by the antiserum absorption method. Prototype strains of the influenza A (H3N2) virus can be arranged on a gradient showing the degree of the antigenic drift which the haemagglutinins of the strains have undergone. The demonstration of fine antigenic differences is based on an antiserum absorption test which allows a detailed antigenic analysis of strains. The gradient provides information on variation in strains occurring in different geographical areas and its use may be helpful in differentiating between introduced strains and locally developing variants.


The sequential appearance of antibody and immunoglobins in nasal secretion after immunization of volunteers with live and inactivated influenza B virus vaccines. The sequential development of the immune response in nasal washings was studied in 54 volunteers immunized with either attenuated or inactivated influenza B/Eng/13/65 virus vaccines.Eleven of the 15 volunteers given the inactivated vaccine by deep subcutaneous inoculation showed no rise in nasal wash protein or immunoglobins due to the immunization procedure nor was specific neutralizing antibody detected in their nasal washings after immunization. Neutralizing antibody was detected in nasal washings of three volunteers in this group who also showed a 20-fold or greater increase in serum haemagglutinin-inhibiting antibody after immunization and in one volunteer who had antibody present in pre-trial nasal washings.Eleven of 15 volunteers who were successfully infected by the live attenuated vaccine showed a characteristic rise in protein and IgA and IgG immunoglobin concentrations in nasal washings 5-14 days after the administration of the live virus vaccine. Neutralizing antibody was detected in the nasal washings of these 11 volunteers and appeared at the same time as or 1-2 days after the initial rise of protein and immunoglobin. Neutralizing antibody was also detected in the nasal washings of one other volunteer who did not show a rise in protein or immunoglobin concentration in nasal washings after immunization.IgA was detected (>/= 3 mg./100 ml.) in the majority (84%) of nasal wash specimens which had a protein concentration of 0.2 mg./ml. or greater while IgG was not detected (>/= 4.5 mg./100 ml.) until the protein concentration rose to 0.4 mg./ml. or greater. The geometric mean concentration for normal nasal wash protein in this study was 0.3 +/- 0.1 mg./ml.Regression analysis indicated that the concentrations of both IgA and IgG immunoglobins were directly proportional to the protein concentration in nasal washings but that this relationship varied considerably between individuals.Absorption studies indicated that neutralizing and haemagglutinin-inhibiting antibodies in nasal secretion to influenza B/Eng/13/65 virus were predominantly associated with the IgA class of immunoglobin.


Fluctuations in the titre of antibody to a soluble antigen of myxoma virus in field populations of rabbits, Oryctolagus cuniculus (L.), in Australia. Results are presented on tests carried out over the past 5 years to measure the antibody titre of various rabbit populations to a soluble antigen of myxoma virus. In an unexpectedly high percentage of immune rabbits the antibody fell below measurable titres, and later rose to measurable titres without the advent of an observable epizootic of myxomatosis. The re-stimulation of the immune response is discussed in terms of re-infection and virus reactivation.


Isolations of subgenus 3 salmonellas (arizonas) in Cardiff, 1959-1971. Isolations of subgenus III salmonellas (arizonas) made in the regional Public Health Laboratory, Cardiff, between 1959 and 1971 are reviewed. The techniques of isolation are listed for the various materials examined. The necessity of using bismuth sulphite agar as a plating medium is stressed, as some strains might appear on brilliant green MacConkey agar as rapid lactose fermenters and be missed. The serotypes isolated in Cardiff are discussed with reference to isolations by other authors. The culture of a subgenus III salmonella from pig faeces is described. It is thought that this is the first record of such an isolation from a pig in the United Kingdom.


Typhoid fever imported from Mexico to Switzerland. Studies on R factor mediated chloramphenicol resistance. A case of typhoid fever caused by Salmonella typhi occurred in Geneva. The patient was probably infected in Mexico City. The strain isolated from this patient corresponds with the description of the Mexican S. typhi strain, since it is a degraded Vi-strain resistant to chloramphenicol, streptomycin, sulphonamides and tetracyclines. It carried an fi(-) transferable R factor with a CSSuT resistance pattern. It can be accepted that this case forms part of the Mexican outbreak of chloramphenicol-resistant typhoid fever which has already been observed in visitors to Mexico from England and the United States.


Salmonella dublin abortion in cattle. II. Observations on the whey agglutination test and the milk ring test. The serum and whey agglutination test were compared on paired samples from thirty-five cases of bovine abortion associated with Salmonella dublin infection. The whey test proved nearly as useful as the serum test for confirming an active infection though it was only practicable to examine the whey for flagellar antibodies. S. dublin was isolated from nearly half of the milk samples obtained within the first week of abortion but none of those collected after the fourth week. The whey test proved of no value in retrospective identification of abortion cases. The trial using the milk ring test was disappointing.


Interactions of TRIC agents with macrophages and BHK-21 cells observed by electron microscopy. TRIC agents do not multiply in mouse peritoneal macrophages in culture but have a toxic effect on them, whereas they multiply readily in BHK-21 cells. Sections of macrophages and of BHK-21 cells fixed during the first 90 min after inoculation were examined by electron microscopy. Macrophages ingested all forms of the organism, which were eventually degraded in lysosomes. However, elementary bodies were distinguished from other TRIC particles by the delay in their transfer to lysosomes. BHK-21 cells ingested elementary bodies selectively, and in these cells the organisms were neither found in lysosomes nor degraded. Instead they showed morphological changes that probably represented an early stage of development.


A bacteriological evaluation of laminar-flow systems for orthopaedic surgery. An evaluation has been undertaken of the efficiency of laminar-flow ventilation in operating-rooms in which conventional operating-room clothing was used. It has been demonstrated that velocities in the region of 0.3-0.4 m./sec. will give maximum returns for effort in both down-flow and cross-flow systems. At this velocity the laminar-flow system, in terms of airborne bacteria measured at the wound site, was about 11 times more efficient using horizontal air-flow and 35-90 times more efficient using vertical air-flow than a plenum-ventilated operating-room.


Hong Kong influenza in the Royal Air Force 1968-70. A prospective serological and clinical study of the epidemics due to the A2/Hong Kong/68 influenza virus was made during the winters 1968-9 and 1969-70 in volunteer subjects in the Royal Air Force. In October 1968 nearly all subjects had haemagglutination inhibiting (HI) antibody to the A2/Singapore/57 virus and more than half had antibody to strains more recently prevalent in Britain. The proportion with HI antibody to A2/Hong Kong/68 increased from 31% in October 1968 (most at low titres) to 44% after the first epidemic and 72% after the second (most at high titres). Serological infection rates were much lower in those who had detectable antibody at the beginning of each winter than in those who did not. Respiratory illnesses coupled with serological evidence of influenza infection during the winter were rare in persons with an initial titre of HI antibody of 1/40 or more. Infection in the first winter conferred complete protection against infection, with or without illness, in the second. In both epidemics about half those with serological evidence of infection had no reported illness.


Thermal comfort zones obtained by two alternative.