Genetic control of  -glycerolphosphate dehydrogenase in mouse brain. Activity of alpha-glycerolphosphate dehydrogenase (EC 1.1.1.8), linked to cytoplasmic nicotinamide adenine dinucleotide, is higher in brains of BALB/cJ mice than in brains of C57BL/6J mice. The magnitude of the difference is dependent on both the age and brain region of the mice. Maximal differences are observed at 20 days of age and are detected in the cerebellum, with only marginal differences occurring in other brain regions. Kidney and liver of BALB/cJ mice have higher enzyme activity than those of C57BL/6J mice, whereas muscle-enzyme activity is lower in BALB/cJ mice than in C57BL/6J mice. In addition to differences in activity, alpha-glycerolphosphate dehydrogenase in BALB/cJ mice is more sensitive to heat denaturation. The half-life for the enzyme at 50 degrees in BALB/cJ, (BALB/cJ x C57BL/6J)F(1) hybrid, and C57BL/6J mice is 1, 1.7, and 3 min, respectively. Genetic analysis of the mode of inheritance of the heat-sensitive enzyme reveals that the trait is determined by a single autosomal gene. Genetic analysis of the activity of alpha-glycerolphosphate dehydrogenase in the cerebellum indicates that the mechanism of inheritance for this trait involves two or more genes and that one of these genes also confers heat sensitivity on the enzyme. The affect of these genes on enzyme activity is codominant and additive.


Structure of the oxidized form of a flavodoxin at 2.5-Angstrom resolution: resolution of the phase ambiguity by anomalous scattering. Flavodoxin from Desulfovibrio vulgaris crystallizes in the oxidized form as well-formed, tetragonal bipyramids, space group P4(3)2(1)2, unit-cell parameters, a = b = 51.6 A, c = 139.6 A, 8 molecules per unit cell. The structure has been determined at 2.5-A resolution with phases based on a single isomorphous derivative. The phase ambiguity of a single derivative was resolved by use of anomalous scattering from the single-site Sm(+3). The molecule has a five-strand pleated sheet core with two long helices on either side of the sheet. The flavin mononucleotide lies mostly buried on one side of the molecule, but the methyl groups, one edge of the flavin, and part of the ribityl are exposed at the surface.


Acetylcholine receptors of muscle grown in vitro. [(125)I]Monoiodo- and [(125)I]diiodo-alpha-bungarotoxin were synthesized and shown to bind specifically to the acetylcholine receptor of cultured embryonic chick- and rat-muscle cells. The pharmacologic properties of the receptor of cultured embryonic chick muscle resembled those of the nicotinic acetylcholine receptor of adult vertebrate muscle. Autoradiography of muscle cells labeled with toxin showed that acetylcholine receptors were distributed over the entire cell surface. In addition, discrete areas with a high receptor concentration were found.


Nuclear magnetic resonance determination of the angle psi in peptides. A simple theoretical model is used to suggest that the NCCH coupling constant, Jnh, has a dihedral angle dependence that can serve for estimating the angle Psi in peptides. Some experimental comparisons are made to test the model; they include a new measurement of Jnh in N-acetylglycine.


Structure of the radical form of clostridial flavodoxin: a new molecular model. Interpretation of a new electron-density map at 3.25-A resolution has led to a somewhat revised model for the free radical (semiquinone) structure of flavodoxin from Clostridium MP. Although the general conformation of the molecule is very similar to that of oxidized Desulfovibrio vulgaris flavodoxin, flavin mononucleotide-protein interactions are not identical in the two flavodoxins. In the Cl. MP semiquinone molecule, the isoalloxazine ring appears to retain the essentially planar conformation characteristic of oxidized flavins; within the limits imposed by the resolution of the data, the map shows no evidence for bending of the isoalloxazine ring about N5-N10.


Blocking by histones of accessibility to DNA in chromatin: addition of histones. The blocking effect of histones on accessibility of DNA to DNase has been studied by addition of various histones to chromatin in intact or histone-depleted thymus nuclei (those from which all lysine-rich histone was removed and those from which much of the arginine-rich histone was removed) and to deoxyribonucleoprotein (chromatin) extracted from nuclei. In each case lysine-rich histone, weight for weight, blocks accessibility more effectively than does arginine-rich histone. Fractions of the arginine-rich histone differ considerably from each other in their blocking efficacy. Phosphorylation of lysine-rich histone tempers its blocking effectiveness.


A particle associated with the polyadenylate segment in mammalian messenger RNA. A structure consisting of poly(A) complexed with other components is released from polysomes by ribonuclease treatment. The poly(A) complex has a sedimentation value of 12-15, while the corresponding sedimentation value for free poly(A) is 4. The complex does not appear to represent an artifact formed by interaction of free poly(A) with either cytoplasmic or polysomal proteins. The polynucleotide released from the complex by treatment with sodium dodecyl sulfate shows the same electrophoretic mobility as that of poly(A) isolated from deproteinized polysomal RNA. The poly(A) in the complex is partially protected from digestion by T(2) ribonuclease. At least part of the poly(A) is available for base pairing with poly(U). The components associated with the poly(A) cause it to bind to Millipore filters at low ionic strength. These components are removed from the complex by Pronase digestion. The findings indicate that the poly(A) segment in messenger RNA serves as a binding site for a particle. This particle appears to consist of proteins.


Production of procollagen by human fibroblasts in culture. Three hydroxyproline-containing proteins secreted into the medium by human fibroblasts in culture were isolated and characterized. A minor fraction was identical to the collagen monomer. The major fraction was a form of procollagen, which contained, in addition to pro alpha and alpha chains, a component estimated to have a molecular weight of 250,000. This component was a dimer of pro alpha chains joined by disulfide bonds. The third fraction, much lower in hydroxyproline and hydroxylysine content, was of still greater size. Pro alpha chains were released upon denaturation and reduction, indicating that this fraction may contain pro alpha chains linked by disulfide bonds to noncollagenous material.


Genetics of somatic mammalian cells: demonstration of a human esterase activator gene linked to the adeB gene. Prototrophic hybrids formed from an adenine-requiring Chinese hamster cell and human fibroblasts uniformly display new esterase activity that differs from that of either parental cell in electrophoretic mobility and substrate specificity. The hybrids that grew in the selective medium and possessed the new esterase activity had a single extra chromosome that resembled a B-group human chromosome. When clones of such hybrid cells were cultured in nonselective medium, they rapidly reverted to inability to synthesize adenine, disappearance of the new esterase activity, and simultaneous loss of the extra human chromosome. Esterase activity like that of the hybrid is present in cells of various Chinese hamster, but not human, tissues. It is postulated that particular Chinese hamster esterase genes became inactive after longterm cultivation, and that, in the hybrid cell, a human activator gene linked to the adeB gene and located on a human B-group chromosome reactivated expression of these Chinese hamster esterase genes.


The role of polyprenol-bound saccharides as intermediates in glycoprotein synthesis in liver. It has been reported that liver microsomes catalyze the transfer of glucose from uridine diphosphate glucose to dolichol monophosphate so as to produce dolichol monophosphate glucose. Dolichol is a polyprenol containing about 20 isoprene units. The glucosyl residue of dolichol monophosphate glucose is transferred to an endogenous acceptor on further incubation with liver microsomes. The glucosylated endogenous acceptor appears to be an oligosaccharide of about 20 monosaccharide units bound to dolichol through a phosphate or pyrophosphate bridge. In this paper it is reported that liver microsomes catalyze the transfer of the oligosaccharide from the glucosylated endogenous acceptor to an endogenous protein. This transfer reaction requires the presence of bivalent cations, manganese being more effective than magnesium. The presence of deoxycholate is also required. Besides the glycoprotein, several water-soluble products are also formed. Preliminary evidence indicates that they are glucose, iligosaccharides of different size, and possibly oligosaccharides bound to amino acids.


The possible role of aromatic residues of Clostridium acidi-urici ferredoxin in electron transport. The (13)C-resonances of the 2',6'-ring carbon atoms of both tyrosyl residues of Clostridium acidi-urici ferredoxin are shifted downfield in the oxidized and reduced protein relative to these resonance positions in free tyrosine. These results show that both tyrosyl residues in the oxidized and reduced protein are in magnetically equivalent environments, and suggest that both tyrosyl residues are close to the two iron-sulfur clusters in the reduced and oxidized proteins and that each cluster is equally accessible to one reducing electron.


Ab initio calculations on large molecules using molecular fragments. Evidence of hydrogen bonding in polypeptides of glycine. Ab initio calculations on di-, tri-, tetra-, and pentapeptides of glycine in various conformations are reported. Hydrogen bonding is observed as an important stabilizing force in alpha-helical conformations. These studies on polypeptides of glycine of reasonable size show for the first time that the forces acting to stabilize polypeptide conformations can be extracted directly from theoretical studies, without prior postulation of their existence or need for concern that neglect or approximation of various integrals may have biased the results.


Coenzymatic activity of pyridoxal 5'-sulfate and related analogues of pyridoxal 5'-phosphate. The effect of changes in the substituent at the 5'-position of pyridoxal 5'-phosphate on the coenzymatic activity of six analogues of this coenzyme was determined for three bacterial enzymes. Pyridoxal 5'-sulfate showed substantial coenzymatic activity for arginine decarboxylase and tryptophanase, but not for D-serine dehydratase; alpha(5)-pyridoxalmethylphosphonate showed substantial activity for D-serine dehydratase, but not for the other two enzymes. These results demonstrate that neither the dianionic phosphate group nor the ester oxygen of pyridoxal 5'-phosphate is a general requirement for coenzymatic activity. Minor variations in orientation and charge of the group at position 5 exert major effects on the capacity for complex formation between analogue and apoenzyme, and on the catalytic efficiency of the resulting complex, but have comparatively little effect on the substrate affinity of the analogue-apoenzyme complexes. These effects show no general pattern from enzyme to enzyme, and do not correlate with the affinity of analogue for apoenzyme under the conditions tested.


Inactivation of bovine trypsinogen and chymotrypsinogen by diisopropylphosphorofluoridate. Diisopropylphosphorofluoridate reacts with trypsinogen and chymotrypsinogen and inhibits the potential activity of both zymogens. The reactions follow pseudo first-order kinetics and proceed approximately four orders of magnitude slower than diisopropylphosphorylation of the corresponding enzymes. Correlation of initial rates of inactivation with incorporation of the reagent indicates that zymogen inactivation results from incorporation of 1 mol of organic phosphate per mol of protein. Peptides isolated from the active-site region of trypsinogen account for more than 60% of the label originally present in the [(14)C]diisopropylphosphoryl zymogen. It is concluded that loss of activation of trypsinogen is due to alkylphosphorylation of Ser(183). It is proposed that reduced reactivity of the zymogen, as compared to the enzyme, primarily reflects inefficient binding of substrates and inhibitors, and that Ser(183) of the active site exists in trypsinogen in an activated state.


Nuclear magnetic resonance spectrum of deamino-lysine-vasopression in aqueous solution and its structural implications. The peaks in the proton nuclear magnetic resonance spectrum of deamino-lysine-vasopressin in aqueous solution at pH values between 3 and 5 were assigned to particular amino-acid residues by use of the results of transfer-of-saturation studies, NH-C(alpha)H and C(alpha)H-C(beta)H decoupling experiments, and other data. The conformation of deamino-lysine-vasopressin in water differs from that of lysine-vasopressin in the same solvent.


Translational control of hemoglobin synthesis by an initiation factor required for recycling of ribosomes and for their binding to messenger RNA. The continued recycling of ribosomes during protein synthesis in rabbit reticulocyte lysates at 37 degrees requires an initiation factor whose activity is rapidly lost in the absence of added heme. Partially purified factor (i) fully maintains the polysomes; (ii) inhibits the association of 40S and 60S ribosomal subunits into single ribosomes; (iii) promotes the quantitative entry of added 60S subunits into polysomes; (iv) allows the accumulation of ribosomal subunits, instead of single ribosomes, when initiation is blocked with aurin tricarboxylate; and (v) is absolutely required for the binding of globin messenger RNA to ribosomes.These properties suggest that this mammalian initiation factor functions analogously to bacterial IF-3. In addition, the translational control of globin synthesis by heme is exerted, directly or indirectly, through this factor.


Nonhistone chromosomal proteins in synchronized HeLa cells. Chromatin was isolated from synchronized HeLa cells at different stages of the cell division cycle and fractionated into DNA, histones, and nonhistone proteins. Electrophoresis of the nonhistone proteins in sodium dodecyl sulfate-polyacrylamide gels revealed a highly reproducible pattern of 22 bands, having estimated molecular weights of 15,000-180,000, with 85% (by mass) over 40,000. The amounts of some nonhistone proteins varied during the cell cycle by as much as 50%, while others remained at a constant level. One group of nonhistone proteins (molecular weight 75,000) was greatly reduced just before the start of DNA replication (S-phase), then returned to normal levels in the mid-S phase. These results are discussed with regard to the possible role of nonhistone proteins in regulating chromosome structure and function.


Synthesis of proteochondroitin sulfate by normal, nanomelic, and 5-bromodeoxyuridine-treated chondrocytes in cell culture. Chondrocytes grown in cell culture from micromelic chick sterna and 5-bromodeoxyuridine-treated normal cells showed reduced incorporation of sulfate into hyaluronidase-sensitive material as compared to normal chondrocytes. Proteochondroitin sulfate synthesized by normal embryonic chick chondrocytes in cell culture was heterogeneous on chromatography. The major chromatographic component was specifically inhibited in normal chondrocytes treated with 5-bromodeoxyuridine, and this component was absent in nanomelic chondrocytes.


Location of ribosomal DNA in the human chromosome complement. Hybridization of (3)H-labeled ribosomal RNA to human chromosomes on slides resulted in specific labeling of the satellite regions of chromosomes 13, 14, 15, 21, and 22, with an over-all efficiency of about 5%. Differences between D and G chromosomes, and between associated and unassociated satellites, were not significant. Labeling of all other parts of the preparations was nonspecific, and increased in the order: extrachromosomal regions < chromosome arms < centric regions.


Conformational relationships between analogs of acetylcholine and those of local anesthetics in solution. Conformations of analogs of acetylcholine and of related local anesthetics, in which either of the oxygen atoms had been replaced by other heteroatoms, were studied in solution by nuclear magnetic resonance. Several correlations between structure and conformation could be made.


Transfection mediated by Mycoplasmatales viral DNA. DNA isolated from Mycoplasmatales viruses MVL51 and MVGs51 was infectious when mixed with Acholeplasma laidlawii BN1-Na1(R) cells. Infectivity was destroyed by deoxyribonuclease but not by ribonuclease, Pronase, or specific antiserum to the virus. Host mycoplasma cells were only competent for transfection during late-log growth phase. The rates of the establishment of DNase insensitivity of viral DNA transfectants were similar to those of bacteriophage systems. The dose-response curve for transfection suggested that an average of six molecules of DNA must interact with a cell in order to produce one infectious center. Mycoplasmatales virus DNA exhibited a low efficiency of infection; one infectious center required 4 x 10(5) virus equivalents of DNA.


Enhancement of hexose entry into chick fibroblasts by starvation: differential effect on galactose and glucose. Glucose entry, as measured by 5-min uptake into the acid-soluble fraction, is enhanced 15-30 times by long-term (12-24 hr) hexose starvation of chick fibroblasts. The rate of galactose accumulation in the cells increases only 5 times under the same conditions of starvation. Several carbon and energy sources that were tested for their effect on this "derepression" can be classified as: (i) those resembling glucose in blocking the "stimulation," (ii) those permitting full "derepression"; and (iii) those partially preventing the enhanced entry. Inhibitors of protein synthesis block enhancement under conditions otherwise conducive to it. We conclude that the glucose and galactose carrier systems are not identical, based largely on the asymmetric "repression" observed when glucose and galactose are compared as "repressors."


Induction of auxotrophic mutations by treatment of Chinese hamster cells with 5-bromodeoxyuridine and black light. Treatment of an aneuploid Chinese hamster cell line (V79) in tissue culture with 5-bromodeoxyuridine and black light, with or without prior exposure of cells to another mutagen, led to the isolation of auxotrophic mutants, each of which exhibits a specific nutritional requirement for glycine, uridine, purine, or for a combination of glycine, hypoxanthine, and thymidine. 61 mutants that could not use exogenous galactose were also isolated. Thus, this treatment functions as an inductive, but not as a selective, procedure for mutations in mammalian cells.


Kinetic and equilibrium analysis of estradiol in uterus: a model of binding-site distribution in uterine cells. Kinetic and equilibrium binding studies indicate that the process by which the complex of estradiol-binding protein is transferred to the cell nuclei is very rapid and is readily reversible in intact cells; that is, the cytosol and nuclear binding sites are in a rapidly reversible equilibrium. Binding of the hormone appears to shift this equilibrium such that a large percent of the filled binding sites become associated with the nuclear fraction. A model is presented to show that the quantity of filled nuclear binding sites present at any estradiol concentration can be determined strictly by the initial binding between the hormone and the cytosol binding sites.


Enzymic depolymerization of macromolecular heparin as a factor in control of lipoprotein lipase activity. Macromolecular heparin from rat skin shows poor lipoprotein lipase-releasing activity in vivo and is a potent inhibitor of rat-heart lipoprotein lipase in vitro. Rat-skin heparin is depolymerized by incubation with the 100,000 x g supernatant from a sonicated homogenate of rat small intestine. The depolymerized products, fractionated by gel filtration, range from inhibitors to activators of lipoprotein lipase as molecular size decreases. Depolymerized rat heparin in the same molecular size range as commercial heparin from pig intestinal mucosa has about two-thirds the activity of the commercial preparation, both in vivo and in vitro.


Resolution and reconstitution of the phosphatidate-synthesizing system of rat-liver microsomes. The phosphatidate-synthesizing system of rat-liver microsomes was resolved into two component enzymes, glycerolphosphate acyltransferase and 1-acylglycerolphosphate acyltransferase. The resolution is effected by sucrose density gradient centrifugation in the presence of a nonionic detergent, Triton X-100. Combination of both enzymes results in reconstitution of the phosphatidate-synthesizing system. These results establish that two distinct enzymes, glycerolphosphate acyltransferase and 1-acylglycerolphosphate acyltransferase, are required for synthesis of phosphatidic acid from sn-glycerol 3-phosphate.Furthermore, the 1-acylglycerolphosphate acyltransferase preparation efficiently uses unsaturated (or saturated) fatty acyl-CoA as acyl donor. Our previous studies showed that the glycerolphosphate acyltransferase preparation catalyzes formation of 1-acylglycerol 3-phosphate, using preferentially saturated fatty acyl-CoA as acyl donor. These findings indicate that the reconstituted system is capable of yielding phosphatidic acid with an asymmetric fatty acid distribution.


The ultrastructure of megakaryocytes in polycythaemia vera and chronic granulocytic leukaemia.


Studies on operations of recurrent gastric cancer cases.


Specific glycine--accumulating synaptosomes in the spinal cord of rats. Subcellular fractionation of rat spinal cord on continuous sucrose density gradients provides evidence for the existence of a specific synaptosomal fraction (enriched in pinched-off nerve endings) that accumulates glycine selectively by way of a high-affinity transport system. The particles in this fraction sediment to a less-dense portion of sucrose gradients than do particles that accumulate neutral, basic, aromatic, and acidic amino acids. Particles accumulating gamma-aminobutyric acid are even less-dense than those storing exogenous glycine. The glycine-specific synaptosomal fraction also exists in the brain stem but not in the cerebral cortex. These findings provide neurochemical support for the suggestion that glycine has a specialized synaptic function, perhaps as neurotransmitter, in mammalian spinal cord.


Basal metabolism and other physiological changes in wintering members of Japanese antarctic research expedition 1968-1969.


Brain scanning of subdural hematoma in infants and children.


The periodontium of ageing humans.

The periodontium of ageing humans.


Periodontal disease in sheep. II. The composition of sera from sheep with periodontosis.


Oral hygiene and periodontal disease in an adult population in the United States.


Bacteriologic studies of periodontal pockets ten minutes after curettage.


The relation of water pressure cleansing to the reticulo-endothelial system.


Effects of a chlorhexidine gluconate mouthrinse on dental plaque and calculus.


An investigation into the accuracy of measurements made on radiographs of the alveolar crests of dried mandibles.


Allergic response to copper--its possible gingival implications.


Periodontics for the general practitioner.


Treatment of acute leukemia.

Treatment of acute leukemia.


Amputation for osteogenic sarcoma.

Amputation for osteogenic sarcoma.


Radiosensitivity and recovery potential of human blood-forming tissues: comparison of malignant and nonmalignant states.


Physical considerations in sanctuary radiotherapy of acute lymphoblastic leukemia in children.


Riboflavin supplementation in the dog.

Riboflavin supplementation in the dog.


The treatment of acute myelocytic leukemia.


Therapy of acute myelocytic leukemia. Daunomycin contrasted with a combination of cytosine arabinoside and 6-thioguanine. Twenty-three patients with acute myelocytic leukemia (aml) were treated with daunomycin and the results contrasted to those obtained in a subsequent group of 18 patients treated with cytosine arabinoside (ara-c) and 6-thioguanine (tg). The complete remission (cr) rate with daunomycin was 17 percent (mean duration 10.6 months) and the partial remission (pr) rate 26 percent (mean duration 44 days). Corresponding figures in the ara-c and tg group were: cr rate 44 percent (mean duration 5.8 months) and pr rate 17 percent (mean duration 48 days). There were 12 deaths resulting from daunomycin-induced pancytopenia and in ten of the patients who died persistent leukemia infiltrate was found in antemortem marrow specimens or at autopsy. This contrasts with death of six patients from ara-c and tg-induced pancytopenia, in four of whom residual leukemic infiltrate was not evident. Daunomycin alone is deemed not suitable for induction of remission in aml. The results obtained with ara-c and tg are encouraging and may be improved if the number of infectious deaths associated with drug-induced pancytopenia can be reduced.


Longterm support with a membrane lung.

Longterm support with a membrane lung.


A proposed new classification of macrophages, monocytes and their precursor cells.


Interocular transfer of adaptation to spatial frequency during retinal ischaemia.


Gene duplication in carp muscle calcium binding protein.


Complex cell response depends on interslit spacing.


Gene triplication deduced from the tertiary structure of a muscle calcium binding protein.


Therapeutic effect of kinetin on tobacco alternariosis.


Bacteriophage T4 tRNA Leu .

Bacteriophage T4 tRNA Leu .


Smoking habits and respiratory symptoms of Dunedin public servants.


The general practitioner and negligence.


XXXXY syndrome with mosaicism: case report.


The effects of infant feeding on speech quality. In an effort to examine retrospectively the influence of bottle feeding compared with breast feeding, a speech survey was carried out on 131 5- and 6-year-old European boys and girls from 2 schools in Putaruru, New Zealand, 1 state and the other parochial.  Speech assessments were all made before information was gathered on the feeding regime in order to avoid any possibility of bias.  Each child was interviewed alone for a period of 6-40 minutes.  An assessment of his/her speech was made under the following headings:  articulation; tonal quality; and fluency.  Speech defects such as substitutions, omissions and intrusions, denasalization and positive nasalization were analyzed and recorded phonetically on each child's record form.  Notes were also made regarding the effect of missing teeth or malocclusion, auditory perception, substandard speech, and tonal quality-phonatory defects.  There was a distinct relationship between breast feeding and clarity of speech in the male.  Breast feeding was associated with improved tonal quality in both male and female children with a more marked improvement in the case of the male.  Improved speech was associated with improved reading ability.  The feeding regime was found to have no effect on a child's confidence.  The dramatic effect of breast feeding on the development of speech and reading in boys as revealed in this study suggests that the natural breast feeding method accelerates the rate of maturation of the male infant.


Methadone and addiction.

Methadone and addiction.


Congestive heart failure.

Congestive heart failure.


The Royal Commission on Hospital and Related Services: item 9 of the warrant--submissions.


Nursing shortage in public hospitals of New Zealand: Letter No. 3 to Members of Parliament.


Smoking and health: the 1972 situation.

Smoking and health: the 1972 situation.


Neonatal blood tyrosine elevations.

Neonatal blood tyrosine elevations.


Connective tissue diseases.

Connective tissue diseases.


Testing for diabetes.

Testing for diabetes.


Laxative-induced hepatitis--oxyphenisatin.


Human leukemic cells. Fractionation and characterization of ribonucleic acids.


Steroid hormone induction of a specific translatable messenger RNA.


Non-linkage of induced mutations in Chinese hamster cells.


Detection of single gene effect by inbreeding.


X-linkage of human  -galactosidase.

X-linkage of human  -galactosidase.


Cytochalasin B fails to inhibit pinocytosis by macrophages.


Lack of differences in serum binding of calcium in cystic fibrosis, carriers and controls.


Cell response to surface stimulation.

Cell response to surface stimulation.


Synthesis of two natural salmon calcitonins.


Granulomatous diseases of small and large bowel.


X-ray and optical diffraction approach to skin structures.


Emotional considerations in cancer and stroke. In cancer.


Emotional considerations in cancer and stroke. In stroke.


Preoperative withdrawal symptoms with methadone maintenance.


Ultrasonic therapy for chronic acromioclavicular separation with calcific deposits.


Bigeminy and heroin intoxication.

Bigeminy and heroin intoxication.


Basilar artery thrombosis. Electrocardiogram simulating acute infarction.


Nutritional megaloblastic anemia in vegan.


Gastric perforation in premature newborn. Operation and survival.


Peripheral neuritis after methimazole therapy.


Family with congenital heart disease.

Family with congenital heart disease.


Elective abortion. Woman in crisis.

Elective abortion. Woman in crisis.


Coronary and left-heart angiography in assessment of chest pain.


Detoxification of multiple-drug abusers with sodium amytal.


Anomalous inferior vena cava. Azygos continuation producing hilar tumor.


Barrett's syndrome?

Barrett's syndrome?


Slow detoxification in heroin addiction. Combined use of methadone and psychotherapy.


Reversibility in agnogenic myeloid metaplasia.


Neonatal hamartoma with consumption coagulopathy.


Postpancreatectomy insulin-resistant diabetes mellitus. Improvement after prednisone and 5-fluorouracil.


Transplantation glossary.

Transplantation glossary.


Milk and blood alcohol.

Milk and blood alcohol.


Emergency room crisis and challenge. Technic for self study and change.


Accidents to nursing staff in a psychiatric hospital.


Niflumic acid in rheumatoid arthritis: a pilot study.


Superior mesenteric artery occlusion.

Superior mesenteric artery occlusion.


Law and the doctor.

Law and the doctor.


The effect of cholecystectomy upon renal excretion rates of ribonuclease, lysozyme and total protein.


The health centre: a revolution in medical education?


Smoking and coronary heart disease.

Smoking and coronary heart disease.


Medical Research Council report on marijuana.


Stock's cohort death rates for cancer of the stomach fitted by a two-step origin of the cancer.


Nasal tumours in starved rats injected once with dimethylnitrosamine.


Clonal evolution in leukaemia.

Clonal evolution in leukaemia.


NZNCRL pathologic rubric for computer processing of animal tumor data.


Clonal origin of the Philadelphia chromosome and chronic myeloid leukaemia: evidence from a sex chromosome mosaic.


Logical structure of a direct access library for computer reduction of oncologic data.


The formation of carcinogenic nitrosamines in vivo: studies using combined treatment with piperazine and nitrite.


Specialists in additional steps.

Specialists in additional steps.


Nursing education.

Nursing education.


Alcoholism.

Alcoholism.


Ceylon medical degrees.

Ceylon medical degrees.


Pregnancy under 16.

Pregnancy under 16.


Ocular complications of the pill.

Ocular complications of the pill.


Relation between selected predictor variables and grades in fixed prosthodontics laboratory.


Isolation of vitamin B12-binding proteins using affinity chromatography. II. Purification and properties of a human granulocyte vitamine B12-binding protein.


Senior elective program in dentistry.

Senior elective program in dentistry.


Continuous-progress education. II. Computer simulation model of a flexible dental curriculum.


Clinic management through group administrators.


Teaching of statistics in dental schools.


AFDE Program support to reach 5 million dollars for 1972.


Robert Wood Johnson Foundation grants  4 million to dental schools.


Johnson Foundation makes landmark grant of  4 million to dental schools.


American Fund makes grant of  40,000 to journal for 1973.


Factors which influence cooperation between dentist and child.


Central and peripheral neural influences on the oxytocin-induced pressure response of the mammary gland of the anaesthetized lactating rat.


Psychologic impact of a coronary care unit.


Erroneous diagnoses on admission of chest cases. A pattern of care study.


Understanding African students in the United States.


Symposium on tonsillectomy-adenoidectomy and otitis media.


Career commitments in medicine.

Career commitments in medicine.


Countdown on national health insurance.

Countdown on national health insurance.


Maternal and infant care project.

Maternal and infant care project.


Spontaneous pneumothorax in metastatic osteogenic sarcoma.


Long-term survival rates in cancer.

Long-term survival rates in cancer.


Pneumonitis in acute lymphatic leukemia during methotrexate therapy.


The current status of pour resins.

The current status of pour resins.


A technique for verifying centric relation at the established vertical relation.


A modified sanitary pontic.

A modified sanitary pontic.


Centric-relation recording techniques--a comparative investigation.


Immediate restoration of pulpless teeth.


Pulpal response to composite-resin materials.


A compensating obturator.

A compensating obturator.


Differentiation of temporomandibular joint dysfunction to simplify treatment.


Relining the complete maxillary denture.


Guidelines for the technician in mounting maxillary casts utilizing a modeling-compound face-bow record.


Use of silicone in relining, rebasing, and duplication of dentures.


Artificial tooth arrangement for prognathic patients.


Muramidasuria and hyperkaluria in the chloroleukemic rat.


The effect of serum from patients with acute granulocytic leukemia on granulocyte colony formation in vitro: a search for inhibitors.


Pulmonary hypertension in lung disorders.


Pulmonary hypertension in congenital shunt lesions. Observations in a right heart catheterization material, with particular reference to occurrence, natural history and prognostic implications in adolescence and adulthood.


Proximo-gingival overhangs on premolar amalgam fillings.


Incidence of salt-induced hypertension in rats from different stocks.


The induction of multiple large bowel neoplasms in mice.


Two b-c translocations specifically identified by 'banding' techniques as 46, XY,t(5p+6q-) and 46,XX,t(4q-7p+).


Activation of plasminogen with insoluble derivatives of urokinase.


Considerations of the amnion as an autograft and as an allograft in sheep. A preliminary report.


Adenosine deaminase inhibition by N 6 -(2 -isopentenyl) adenosine.


The physiological disposition of fenoprofen in man. IV. The effects of position of subject, food ingestion and antacid ingestion on the plasma levels of orally administered fenoprofen.


Pelvic lipomatosis. Report of three cases.


Biologic false-positive reactions for syphilis as measured by the Treponema pallidum immobilization test.


On the mechanism of tolerance to drugs. Histamine desensitization.


Local passive transfer of cell-mediated immunity between species.


The blocking of delayed hypersensitivity using an in vivo transfer assay.


An acute-leukemia antigen: correlation with clinical status.


Myeloid leukaemia and cot deaths.

Myeloid leukaemia and cot deaths.


What to do about patients with periodontal disease.


Pseudo mandibular prognathism corrected by total maxillary osteotomy.


Eruption cyst: an analysis of its clinical features.


Scarification of the temporal tendon for treatment of chronic sublaxation of the temporomandibular joint.


Recurrent suppurative parotitis treated by intraductal antibiotics.


Autotransfusion of frozen red blood cells in elective oral surgery.


Regional odontodysplasia with associated midline mandibular cyst: report of case.


Primary malignant melanoma of the hard palate: report of case.


Osteoradionecrosis of the maxilla: report of case.


Choroiditis treated by surgical removal of unerupted impacted maxillary third molar: report of case.


Verrucous carcinoma of the palate: report of case.


Physician, heal thyself.

Physician, heal thyself.


Mandibular micrognathia: etiology and surgical management.


Temporomandibular joint pain-dysfunction problems can be solved.


A headholder for oriented temporomandibular joint radiographs.


Speech patterns of edentulous patients and morphology of the palate in relation to phonetics.


Determination of the accuracy of wax interocclusal registrations. II.


Processing changes in complete dentures: posterior tooth contacts and pin opening.


A new self-threading pin.

A new self-threading pin.


Exercise therapy for accurate recording of centric relation.


Radiation docking device.

Radiation docking device.


Audit of quality of dental care; a pilot-study.


Improvement in oral hygiene as a function of applied principles of behavioral modification.


Abolition of the right of local Swedish authorities to fluoridate drinking water.


The role of dentistry in the neighborhood health center.


Patients and consumer's acceptance of preventive dentistry.


Leukaemia.

Leukaemia.


Crises in education.

Crises in education.


Use and abuse of antibiotic combinations.


Transnasal fiberoptic bronchoscopy.

Transnasal fiberoptic bronchoscopy.


Dental education in Canada.

Dental education in Canada.


The theory of dental administration.

The theory of dental administration.


The three-year dental curriculum.

The three-year dental curriculum.


Historically, when and by whom was silver amalgam introduced?


Improving health science education and training.


Implants.

Implants.


Diagnosis of oral vesicular lesions.

Diagnosis of oral vesicular lesions.


Reports on dental education in Israel.

Reports on dental education in Israel.


The surface is not it.

The surface is not it.


Oral health services in Hong Kong.

Oral health services in Hong Kong.


Excessive or misled pressure on the teeth as etiologic factor of periodontal disease.


The education of dental personnel on the territory of Papua-New Guinea.


People versus pathology.

People versus pathology.


The Australian scene--1972.

The Australian scene--1972.


Dentistry in Kenya.

Dentistry in Kenya.


Dental manifestation of porphyria erythropoietica.


A technical report on periodontal brush-adjunct.


Evaluation of dental and oral disorders in the northern and southern areas of the Nile Valley.


Fluoride levels, fluorosis and dental caries. A study determining the optimal level of fluoride from 15 communities.


Fibrosarcoma of the upper jaw with clinical resemblance to the common periodontal cyst.


The major canals of the mesiobuccal root of the maxillary 1st and 2nd molars.


Expanded duty auxiliaries--why must they be women?


Familial acute myeloid leukaemia with acquired Pelger-Huet anomaly and aneuploidy of C group. All five members of one generation in an Icelandic family were affected by acute myeloid leukaemia or preleukaemia. Two sibs died of acute myeloid leukaemia and another of myelofibrosis associated with leukaemic changes. The other two sibs are alive; one of them (the propositus) has haematological features consistent with preleukaemia; both of them have evidence of an abnormal cytogenetic clone in the bone marrow with 47 chromosomes, an extra chromosome in the C group. The finding of the acquired Pelger-Huët anomaly in the neutrophils of the five affected sibs suggests that a specific genetic cell defect has been transmitted with autosomal dominant characteristics. The presence of immunological deficiencies and an undue susceptibility to oncogenic viruses, as suggested by warts affecting three family members, may have played a part in the pathogenesis of the leukaemic process.


The New York Dental Service Corporation.


The fashionable tooth.

The fashionable tooth.


Complications resulting from dento-surgical operations in the area of the maxillary sinus. Plastic closure in connection with tooth extraction.


Dowel and core foundations using the composite Adaptic.


Oral rehabilitation. 1.

Oral rehabilitation. 1.


The lowest piston for a treatment chair.


Design principles of orthodontic screws. 1.


Toothkeeper program.

Toothkeeper program.


Disposable trays--a rationalization aid for the dental office.


Interesting cases of cysts in the jaws.

Interesting cases of cysts in the jaws.


A comparison of border induction effects during steady and intermittent illumination.


Comparison of cycloplegic and non-cycloplegic ocular component measurement in children.


Retinal luminance and aniseikonia.

Retinal luminance and aniseikonia.


Body imagery in perceptual learning.

Body imagery in perceptual learning.


Error analysis of corneal thickness measurements.


Quantifying the distribution of lactic acid dehydrogenase in the corneal epithelium with oxygen deprivation.


A new technique for objectively plotting retinal landmarks.


A preliminary study of the service life of the "Soflens TM ".


Osteoma of the tongue. Two case reports.


The New York Dental Service Corporation. A report.


Possible dental complications resulting from extensive prostheses.


Plaque control for the dentist--for the patient.


Dental operative consent--the dentist's legal responsibility.


Dental practioners' comments on the treatment of oral cancer.


Myxoma treated by peripheral ostectomy. Six year follow-up.


Congenital scalp defects: report of four cases.


A comparison of doxepin with diazepam and amitriptyline in general practice.


Hodgkin's disease terminating as acute leukaemia: case report and review of the literature.


Manual correction of sciatic scoliosis.

Manual correction of sciatic scoliosis.


Malnutrition in Auckland children.

Malnutrition in Auckland children.


Maori and European deaths.

Maori and European deaths.


The price of protection.

The price of protection.


Blood alcohol and Transport Amendment Act.


Bioavailability and digoxin.

Bioavailability and digoxin.


The effect of breast versus bottle feeding on speech.


Toxicity of wetting agents in laxatives.


Burns in children.

Burns in children.


Malnutrition in the young.

Malnutrition in the young.


Magnesium pemoline and dextroamphetamine: a controlled study in children with minimal brain dysfunction.


Cell adhesion during the cell life cycle.


Variation of colony morphology reflecting fimbriation in Moraxella bovis and two reference strains of M. nonliquefaciens.


Fimbriation associated with the spreading-corroding colony type in Moraxella kingii.


Competence in genetic transformation related to colony type and fimbriation in three species of Moraxella.


Identification of penicillin-stimulated staphylococcal haemolysin as alpha-lysin.


The effect of mitomycin C on DNA synthesis in P-388 cells.


Protein A in Staphylococcus aureus strains of human and bovine origin.


Semliki forest virus glycoproteins and canavanine.


Reassociation of reduced IgM to covalently bound 8S and 17S molecules.


Temperature optimum and rabbit blood cell culture.


Delirium tremens and certain other acute sequels of alcohol abuse. A comparative clinical, social and prognostic study.


Prognosis of acute myeloblastic leukemia: chromosomal correlation.


Cell kinetics in human acute lymphoblastic leukemia: computer simulation with discrete modeling techniques.


Intravenous radionuclide angiography prior to closed needle biopsy of focal lesions.


Methaemoglobinaemia following prilocaine local anaesthesia.


Unusual causes of respiratory difficulty in the newborn: neonatal chylothorax and neonatal ascites.


Rectal duplication: an unusual congenital anomaly.


An unusual case of large bowel obstruction.


The classification and diagnosis of diabetes mellitus.


The early treatment of cancer of the breast.


Muscle paralysis and herpes zoster.

Muscle paralysis and herpes zoster.


Three years' experience of the working of the Abortion Act in Britain.


Anecdotal acupuncture.

Anecdotal acupuncture.


Personal characteristics of psychiatric student nurses.


Multi-centric clinical trial of a vasoconstrictor-ornithine vasopressin (POR 8 Sandoz).


Unprogrammed reinforcement of children's behaviour in a psychiatric ward.


Non-tuberculous mycobacterial infections.


Problems of tuberculosis management in Sabah.


HL-A antigens and disease. Acute lymphocytic leukemia. 50 Caucasian children with acute lymphocytic leukemia (ALL) and 219 members of their families have been genotyped for 15 antigens of the HL-A system. The antigen and gene frequencies for HL-A2 were significantly higher in the patient population than in a 200 member normal Caucasian panel. No other antigen frequencies were significantly elevated. All antigens typed for were found in the patients. No antigen gain or loss was detected in the leukemic cells.


Dental pulp pathosis. Clinicopathologic correlations based on 109 cases.


Reaction of the rat molar pulp to direct capping with the separate components of Hydrex.


Reliability of panoramic radiography in the diagnosis of maxillary sinus pathosis.


Radiologic evidence of surgical emphysema. Report of a case.


Unusually positioned mandibular molar.

Unusually positioned mandibular molar.


Asymptomatic radiopacity.

Asymptomatic radiopacity.


Duplication of the mandibular canal.

Duplication of the mandibular canal.


Collagen sponge--subcutaneous implantation.


Macroscopic satellite cyst formation in the odontogenic keratocyst. Report of two cases.


Injury to the permanent tooth germ after trauma to the deciduous predecessor.


Mercury excretion in military dental personnel.


Aneurysmal bone cyst. Report of a case.

Aneurysmal bone cyst. Report of a case.


A radiographic study of the point of endodontic egress.


Lymphoepithelial (branchial) cyst of parotid gland.


Lymphoepithelial cysts of the oral mucosa.


Follicular choristoma of the gingiva: a peculiar lesion.


An ultrastructural study of dentinal innervation in the adult human tooth.


The new health.

The new health.


Bullous pyoderma gangrenosum and leukemia.


Symposium on the teaching of tropical medicine. Introduction.


Tropical medicine. What it is not, what it is.


Tropical medicine in a temperate climate: the philosophy and reality of one course.


Teaching and research in tropical medicine in the United States.


Training the physician for development.

Training the physician for development.


Tropical health care system in transition: teaching tropical medicine in a developing country.


Auxiliaries and primary medical care.

Auxiliaries and primary medical care.


Why medical auxiliaries in the tropics? Lessons of a meaningful past.


Adaptation to and continued education for teachers of tropical medicine.


Tropical health care system in transition: training the new generation of key professionals in the promotion of tropical health.


Dental asepsis.

Dental asepsis.


The use of thermography in the pediatric patient. A new diagnostic tool.


The causes of tenosynovitis in industry.


Air levels of lead inside and outside of homes.


A psychiatrist looks at drug abuse.

A psychiatrist looks at drug abuse.


Traumatic and post-traumatic neuroses.

Traumatic and post-traumatic neuroses.


The acute oral toxicity and the hazard associated with the oral administration of two detergents.


Experiments in human work capabilities under pressure, now being conducted at the royal Naval Physiological Laboratory.


Incidence of chromium-induced lesions among electroplating workers in Brazil.


Injury rates by industry 1970, BLS Report 406, Department of Labor, Bureau of Labor Statistics.


A survey of current professional concepts and opinions about the myofascial pain-dysfunction (MPD) syndrome.


A simplified venting procedure for cementation of full veneer crowns.


Certification program for dental materials: List of Certified Dental Materials revised to January 1, 1973. Council on Dental Materials and Devices.


List of Classified Dental Materials and Devices revised to January 1, 1973.


Oral cancer knowledge: results of a survey of Pennsylvania dentists in 1971.


Accredited dental schools. Council on Dental Education.


Cost of conducting a dental practice, 1958-1970. I. Bureau of Economic Research and Statistics.


Course listing for January 1973 through May 1973.


New policies on dental care programs. Council on Dental Care Programs.


Intraoral radiography.

Intraoral radiography.


More on dental licensure.

More on dental licensure.


Are ethical dentists ethical?

Are ethical dentists ethical?


Dental hypnosis.

Dental hypnosis.


Dentistry and politics.

Dentistry and politics.


American Dental Association Principles of Ethics with official advisory opinions as revised November 1972. Judicial Council.


General statement of advertising and exhibiting policy.


Contraception. I.

Contraception. I.


On asymmetric development of the first and second permanent molars in children with cranio-facial anomalies. An orthopantomographic study.


Quantitation of transmitter noradrenaline in the pulps of cat canine teeth.


Laboratory studies on topical treatment of human dental enamel with amine fluoride and acid phosphate fluoride solutions.


A comparative study of tooth formation in the permanent dentition of Finnish children with cleft lip and palate. An orthopantomographic study.


On the macrominerals of deciduous teeth.


Lingual ascorbic acid test and gingivitis.


Hydrolytic enzymes of human blood monocytes and neutrophils with special reference to lysozyme (muramidase).


Venous occlusion plethysmography in 55-year old men. A population study in Malmö, Sweden.


The advantages and disadvantages of removable appliance therapy.


A computerized appraisal of the high-pull face-bow.


Therapy for mandibular dysfunction in adolescents and adults.


Restoring mandibular arch length in the mixed and early permanent dentition.


The Journal's year.

The Journal's year.


A comparison of selected dental cements: effect of film thickness on strength.


Optimal time and temperature for maximum moment and springback and residual stress relief of stainless steel wire.


A study investigating the effect of oblique and cervical headcaps on the size and position of the palatal complex in skeletal open-bites.


Preventive orthodontics: early reduction of Class II, Division 1 malocclusion.


A home-care program of chemical preventive dentistry for orthodontic patients.


Philosophies distinguishing the Begg technique.


Sequential tissue response and recovery after orthodontic tooth movement in hamsters.


American Association of Orthodontists announces results of manpower survey.


Computers--is there a middle road?

Computers--is there a middle road?


Correlation of the mode of fibrin polymerization with the pattern of cross-linking.


Titration of the acceptor cross-linking sites in fibrin.


Fibrin cross-linking and heredity.

Fibrin cross-linking and heredity.


Documentation of the plasma factor XIII deficiency in man.


Transfusion therapy in congenital deficiencies of plasma factor XIII.


Hemorrhagic disease associated with inhibitors of fibrin cross-linkage.


The effect of oral contraceptives on fibrin-clot stabilization.


Fibrin cross-linkage, thrombosis and pregnancy.


Clot stabilization and atherosclerosis.

Clot stabilization and atherosclerosis.


Our ancient heritage in blood clotting and some of its consequences.


Fibrin-stabilizing factor inhibitors.

Fibrin-stabilizing factor inhibitors.


Carboxyl-terminal structures of mammalian fibrinogen and fibrin.


Physiological inhibition of factor XIII.


Polypeptide chains of human fibrin: molecular organization in cross-linking.


The utilization of the Harlow ventilator in anaesthesia.


The involvement of an insurance company in new forms of health care delivery.


Structure and catalytic properties of hepatic transglutaminase.


Marihuana and drug abuse. Recommendations of the Committee on Public Health, New York Academy of Medicine.


Serum alkaline phosphatase: total activity and isoenzyme determinations made by use of the centrifugal fast analyzer.


Root canal treatments for primary teeth.


Conservative endodontic treatment of the open apex in three dimensions.


Treatment planning for the distal shoe space maintainer.


Extraction procedures for the child.

Extraction procedures for the child.


Approximating class II amalgam restorations for primary molars.


Crowns for anterior primary teeth.

Crowns for anterior primary teeth.


Your security.

Your security.


Community Health Centres in Canada. Report of the Hastings Committee.


The Tatamagouche project: an experiment in dental education.


Medico-legal aspects of local anaesthesia.


Management of a broken hypodermic needle: report of a case.


Comparison of three cavity base materials under amalgam restorations.


Suggested revisions of North Carolina's laws on involuntary hospitalization for mental illness.


A student's view of the A.M.A. proposals for health care legislation.


Yogurt.

Yogurt.


Busulphan and the lungs. Absence of lung function disturbance in patients treated with busulphan.


Enzyme therapy for inborn errors of metabolism.


Galvanic degradation of amalgam restorations.


Thermal analysis of dental amalgam.

Thermal analysis of dental amalgam.


Viscoelastic properties of soft lining materials.


Zinc oxide-eugenol cements. 3. Infrared spectroscopic studies.


Effects of heat treatment on mechanical properties of two nickel-chromium-based casting alloys.


Enamel dissolution. I. Effects of various agents and titanium tetrafluoride.


Fluoride supplementation during lactation: caries inhibition without reduction in enamel solubility.


Embryonic-fetal toxicity and teratogenic effects of a group of methacrylate esters in rats.


Enamel dissolution. II. Action of titanium tetrafluoride.


Effects of glucose and urea on dental deposit pH in dogs.


Effects of restorative materials on plaque composition.


The different relationship of the sella point to growth sites fo the cranial base in fetal life.


A stabilizing comonomer. II. Stabilization and polymerization characteristics.


Studies in dental health education. I. Effect of antibiotic prophylaxis on the oral hygiene and periodontal health of young men.


Light deprivation and parotid flow in the human.


Coordination of activity of the two bellies of the digastric muscle in basic jaw movements.


Optimum trituration capsule length.

Optimum trituration capsule length.


Studies on hypermuramidasemia in the normal and chloroleukemic rat: the role of the kidney.


Surface structure of zinc phosphate dental cements.


Periodontal effects of a national health service on an immigrant population.


The management of a maxillary molar furcation involvement occasioned by a severe interproximal alveolar defect.


Detection and estimation of the   2  phase in Dispersalloy by electrochemical techniques.


Occlusal contacts and tooth mobility. Females, aged 18-30.


Effects of fluoride pretreatment in vitro on human teeth exposed to citrus juice.


Molar size sequences in a mixed population of the Northwest Argentine Highlands.


A method for evaluating the orifice pressures of oral irrigation devices.


Oral tissue changes in the chronic alcoholic.


Effect of aquaphor ointment on wound healing.


A direct method of esthetic restoration of dysplastic anterior teeth.


Management of a hemophilic with a dental abscess and subsequent root canal therapy and apicoectomy.


Bone resorption induced by epithelial and connective tissue from human gingiva in vitro.


A rationale for diagnosis.

A rationale for diagnosis.


Pulp stones in the rat incisor.

Pulp stones in the rat incisor.


Efficacy of microscopic dissection of attached gingiva.


A pressureless replica technique for use with the scanning electron microscope.


Apical fenestration. A cause for recalcitrant post endodontic pain.


Restoration of previously unrestorable teeth following periodontal therapy.


Effects of various levels of maternal vitamin A deficiency on the craniodental development of the rat fetus.


Oral manifestations of hematological disorders. Report of four cases.


Apicoectomy with retrograde gold foil: a new technique.


Degradation of lactate by Bacterionema matruchotii under aerobic and anaerobic conditions.


Massive osteolysis (phantom bone, disappearing bone). Report of a case with mandibular involvement.


Surface-active factors in the efficacy of chlorhexidine against plaque.


Incidence of diphenylhydantoin gingival hyperplasia.


The epithelial cell rests of Malassez and the genesis of the dental cyst.


Endodontic teaching manpower report.

Endodontic teaching manpower report.


Clinical evaluation of two bitewing instruments.


Midline maxillary cyst complicated by unerupted mesiodens. Report of a case.


One in a million.

One in a million.


Accidental tooth capping.

Accidental tooth capping.


Strain gauge measurements in connection with the extraction of teeth. II. Equipment and method.


Psychosocial aspects in a series of bruxists compared with a group of non-bruxists.


A factor analytic study of psychosocial background in bruxism.


Comparison of the clinical and roentgenological state at the re-examination of root resections.


Lumbosacral nerve injury in fracture of the pelvis. A postmortem radiographic and patho-anatomical study.


Shock complicating acute myocardial infarction. A clinical hemodynamic and therapeutic study.


Treatment of acute myeloblastic leukemia in adults: remission induction with a combination of cyclophosphamide, cytarabine and vincristine. A regimen of intravenous cyclophosphamide, cytarabine and vincristine, given over a four-day period and repeated every two to three weeks, was used to treat 33 patients with acute myeloblastic leukemia. Of the 30 evaluable patients 9/18 previously untreated patients achieved complete remission and two others marked improvement, and 4/12 previously treated patients achieved complete remission. Twelve of 16 patients under the median age of 38 responded while only 3/14 patients over this age responded. There was no difference in response between those with elevated muramidase levels and those with normal levels. Three patients developed a previously unrecognized syndorme of fever, malaise, rash and orbital suffusion. Cytarabine was probably responsible.At least four courses of treatment are required before abandoning this regimen of therapy. Patients who achieve a complete remission and live for more than 150 days spend about 25% of their total survival time from diagnosis in hospital.


Retrospective study and perspectives in the treatment of meningeal leukaemia.


The diagnosis and treatment of organophosphate insecticide poisoning.


A possible relationship between satellite DNA and the evolution of kangaroo rat species (genus Dipodomys).


Procedure for magnification of lethal deletions of genes for ribosomal RNA.


DNA repair in human leukaemic lymphocytes.


RNA stimulated by indole acetic acid.

RNA stimulated by indole acetic acid.


18 O-rich oxygen from land photosynthesis.


Physiological properties of abscission accelerator from senescent leaves.


Low resolution structure of glyceraldehyde 3-phosphate dehydrogenase.


Transcription and translation in bovine spermatozoa.


Factor 8 activity in haemophkilic plasma unmasked by synthetic organic anions.


Photoemf at a single membrane-solution interface specific to lipid bilayers containing magnesium porphyrins.


Meiotic exchange without the synaptinemal complex.


Tissue changes on the marginal pressure side following experimental tooth movement. A histologic, autoradiographic, and scanning electron microscopic study.


Charcot-Leyden crystals in human bone marrow.


Decreased leukocyte alkaline phosphatase in monocytic leukemia.


The value of roentgenography in differentiating aplastic anemia from leukemia masquerading as aplastic anemia.


Experimental antitumor activity and preclinical toxicology of mycophenolic acid.


Incorporation of uridine- 3 H and sodium acetate- 14 C in lymphocytes derived from normal and leukemic individuals.


Antigenic alteration of lymphoblastic leukemic cells induced by cell culture and hydrocortisone.


The effect of pH on the interaction of fluoride and salivary ions.


Circadian variations in calcium and phosphate secretion from human parotid and submandibular salivary glands.


Chemical analysis of the acquired pellicle formed in two hours on cleaned human teeth in vivo. Rate of formation and amino acid analysis.


Effect of rate of gland function on parotid saliva fluoride concentration in the human.


Intra- and interindividual observations on fluoride ion activity and retained fluoride with sodium fluoride mouth rinses.


Effect of infant diets with widely different fluoride contents on the fluoride concentrations of deciduous teeth.


Effect of fluoride and other trace elements on calcium phosphate precipitates.


Orthophosphates. XIV. Thermodynamical factors influencing the stability of dental enamel to decay.


Orthophosphates. XV. A suggested mechanism for the inhibition of dental caries by monofluorophosphate.


The plaque-inhibiting activity of surface-active substances--sodium N-lauroylsarcosinate and sodium sulphoricinoleate.


Maxillary sinuses and the oral radiologist.


The structure of concanavalin A at 4 A resolution.


Photomicrography in dentistry. New kodak film now available.


Many actions on dental education taken by ADA House of Delegates.


Radiation dose-time relations for induction of osteosarcoma in mice and dogs and their bearing on maximal permissible burden of  90 Sr in man.


Curriculum reforms in medical education.


Students' costs for a dental education in a three-fiscal-year program.


A survey of intramural dental practices in United States Dental Schools.


Using behavioral objectives in new approach to teaching dental anatomy.


Use of macro-models in teaching of tooth preparations.


One instructor's experience with television in dental teaching.


Five-year study of predental and premedical students at University of Iowa.


First union of dentists established in New York.


Survey of attitudes on dental licensing procedures: full disclosure? (2).


On choosing a bull for use in artificial insemination.


A psychosomatic study of bruxism with emphasis on mental strain and familiar predisposition factors.


Sensory thresholds in discriminating differences in thickness between the teeth, by different degrees of mouth opening.


Reduction of vertical overbite in the incisor region effect of a bite plane in dogs.


Relationship between malocclusion and socio-economic level in Finnish rural children.


Observations on tooth mobility in rheumatoid arthritis.


Malocclusion and childhood health.

Malocclusion and childhood health.


The relative incidence of cysts of the jaws.


Eosinophilic leukemia with fibrosing endocarditis and short Y chromosome.


Urinary oestrogen excretion in women with intra-uterine fetal growth retardation.


Birth defects: a geneticist's review of current concepts.


Ultrasonic film echoscopy for placental localization.


Physiological weight changes in pregnancy in Malaysian women.


Infertility, practice and practicability.


Schistosomiasis in gynaecology--two cases in immigrants in Western Australia.


Urinary oestriol excretion in pregnancies complicated by Rhesus immunization.


Oestriol excretion in the pregnancy complicated by diabetes mellitus.


Disappearance after chemotherapy of blocking serum factors as measured in vitro with lymphocytes cytotoxic to tumor cells.


Platelet and fibrinogen kinetics in the chronic myeloproliferative disorders.


Chemotherapy of acute lymphocytic leukemia of childhood.


Acute myelocytic leukemia in adults.

Acute myelocytic leukemia in adults.


"Total therapy" studies of acute lymphocytic leukemia in children. Current results and prospects for cure.


Chronic myelocytic and chronic lymphocytic leukemia.


C trisomy in bone marrow cells in a case of pre-leukaemic acute myelogenous leukaemia. Remarks on the karyotypic analysis and chemotherapy.


Plaque development and gingivitis in the primary dentition.


Histopathology of the human gingiva (study based on 1269 biopsies).


Acute necrotizing ulcerative gingivitis: a survey of 218 cases.


Type II glycogenosis in adults.

Type II glycogenosis in adults.


Oxymetholone therapy in aplastic and other refractory anaemias.


Aplastic anaemia, oxymetholone and acute myeloid leukaemia.


Odontogenic keratocysts.

Odontogenic keratocysts.


Cancer immunotherapy.

Cancer immunotherapy.


Evaluation of a commercially available carbohydrate solution for oral use in glucose tolerance testing.


Identification of the enzymatic pathways of nucleotide metabolism in human lymphocytes and leukemia cells.


Hypokalemia with normal blood pressure preceding malignant hypertension in a patient with primary aldosteronism (Conn's syndrome).


Metastatic meningeal carcinomatosis. Report of a case and review of the literature.


Complete heart block occurring during right-heart catheterization in a patient with left bundle-branch-block and prolonged P-R interval studied by bundle of His recording and atrial pacing.


Amenorrhea: symptom of endogenous depression.


Diagnostic ultrasound in cardiology.

Diagnostic ultrasound in cardiology.


Failure to disclose risk as malpractice.


The effect of a hemisection on the distribution of acetylcholinesterase and choline acetyltransferase in the spinal cord of the cat.


Hypoglycemia in children.

Hypoglycemia in children.


Restricted structural heterogeneity in antibodies: might different heavy chains have a common light chain?


Mode of action of chemotherapy in vivo on human acute leukemia. I. Daunomycin. The leukocytes of 16 adult patients with acute myeloblastic leukemia were studied by autoradiographic methods to elucidate the mode of action of daunomycin. It was shown that daunomycin, at clinically useful doses, exhibits a cytolytic effect on all leukemic blasts whatever their cell-cycle phase. This cytolytic action affects, however, preferentially S-phase cells. It was shown also that blasts of patients less sensitive to daunomycin or receiving a lesser dose of the drug are temporarily blocked in G(2) phase (delayed mitosis) or in G(2) phase (prolonged generation time). Finally daunomycin appeared to hamper the passage of G(2)-blocked blasts from the bone marrow to the blood, while G(2)-phase cells crossed freely.


Levamisole inhibits and cures a solid malignant tumour and its pulmonary metastases in mice.


Effect of reduced fluoride intake by mice on haematocrit values.


Bicuculline methochloride as a GABA antagonist.


Collagen defect induced by penicillamine.


Selective damage of photosystem I of isolated pea chloroplasts after treatment with ruby laser radiation.


Two new linkage groups in man, both carrying different loci for lactate dehydrogenase and glutamic-pyruvic transaminase.


Care of patients in respirator.

Care of patients in respirator.


Innovative trends in respiratory care.

Innovative trends in respiratory care.


Viewing the past as prologue for changes today and tomorrow.


Recredentialing for relicensure.

Recredentialing for relicensure.


Immunologic aspects of connective tissue diseases.


A 51 year old Caucasian male with onset of sudden abdominal pain.


On the influence of ovariectomy, diethylstilboestrol and progesterone on healthy and chronically infected bovine udders.


Bone lesions with giant cells: problems in differential diagnosis.


Association of arginine-rich histones with G-C-rich regions of DNA in chromatin.


Giant RNA transcript in a Balbiani ring.


Antibodies to a DNA:RNA hybrid in systemic lupus erythematosus measured by a cellulose ester filter radioimmunoassay.


Presence and biosynthesis of a polyprenol-type compound in Ascaris lumbricoides (human).


Antigenicity of radiostrontium-induced osteosarcomas.


Experimental gastric ulcer in Shay rat. II. Healing of gastric ulcers, development of their vascular supply, and effect of vagotomy on healing.


Mental state and temporal lobe epilepsy. A correlative account of 100 patients treated surgically.


Toxoplasmosis. Clinical experiences in a cancer hospital.


Spitting automatism in temporal lobe seizures with a brief review of ethological and phylogenetic aspects of spitting.


Changes in interictal focal activity and spike-wave paroxysms during motor and mental activity.


Influence of diphenylhydantoin and phenobarbital on intestinal calcium transport in the rat.


Novel treatment procedures used at an urban treatment center for low-income children with brain dysfunction.


Benign epilepsy of children with Rolandic (centro-temporal) paroxysmal foci. A clinical entity. Study of 221 cases.


Hypno-behavioral approach for the non-consummated marriage.


Group relaxing hypnosis.

Group relaxing hypnosis.


Psychosomatic approaches in Japan.

Psychosomatic approaches in Japan.


The epileptic; a study in status and role.


Birth control and the low income woman.

Birth control and the low income woman.


Establishing a therapeutic climate for geriatric care.


Thoughts about a more open society.

Thoughts about a more open society.


The adult learner in nursing education.

The adult learner in nursing education.


Are nurses ready for teamwork?

Are nurses ready for teamwork?


Low levels of zinc in hair, anorexia, poor growth, and hypogeusia in children.


Experimental studies on cholera immunization. 1. The response of neutralizing and vibriocidal antibodies in rabbits after immunization with culture filtrate material from V. cholerae.


Growth inhibition and cell enlargement in P-388 cells treated with mitomycin C.


Evaluation of neutrophil granulocyte functions.


Haematological changes after injection of endotoxin into warfarin-treated rabbits.


Structural role of RNA in Semliki Forest virus nucleocapsid.


A polysaccharide antigen of an anaerobic oral filamentous microorganism (Eubacterium saburreum) containing heptose and O-acetyl as main constituents. 3. Some serological properties.


Gliding and twitching motility of bacteria unaffected by cytochalasin B.


Tissue hyperosmolality as a mediator of vasodilatation and transcapillary fluid flux in exercising skeletal muscle.


Second premolar extraction in Class I and Class II.


Thumb- and finger-sucking: its relation to malocclusion.


The acceptability of Steiner's acceptable compromises.


Oral surgeon stresses orthodontic care in dental health column.


President's address to the 1972 AAO House of Delegates.


Radiation protection today.

Radiation protection today.


Interceptive orthodontics.

Interceptive orthodontics.


The metric system.

The metric system.


Current concepts in the management of abdominal aortic aneurysms.


Ankylosing spondylitis in women. The disease and its prognosis.


Don't whistle while you work--smoke pipes and cigars?


The role of the physician in sports medicine.


Dalhousie's Family Medicine Centre.

Dalhousie's Family Medicine Centre.


A coronary care unit in a small hospital.


An unusual cause of "surgical acute abdomen".


Breech delivery--help is the answer.

Breech delivery--help is the answer.


Osteoporosis.

Osteoporosis.


Verses on diabetes mellitus.

Verses on diabetes mellitus.


The work load and morbidity in a country general practice.


The drug addict as a patient in general practice.


Thin layer chromatography and the management of drug abuse.


Measurement of pituitary hormones: clinical applications. 3. Growth hormone.


A spectrophotometric artefact in postal specimens of amniotic fluid.


House dust mites: a national survey.

House dust mites: a national survey.


The standard of care.

The standard of care.


The role of the Central Institute of Technology in the training of health-related professionals.


The general practitioner and drug dependence.


The trainee intern.

The trainee intern.


TB and Polynesians.

TB and Polynesians.


Preparation and characterization of several forms of immobilized invertase.


Pharmacology of South American freshwater stingray venom (Potamotrygon motoro).


The use of gas permeability as a molecular probe for the study of cross-linked collagen structures.


Acetylcholine-induced ion transports involved in the formation of saliva.


Leukemia in arsenic poisoning.

Leukemia in arsenic poisoning.


HL-A and lymphoid tumors.

HL-A and lymphoid tumors.


Changing concepts in the treatment of craniofacial anomalies.


A call for new directions in cleft palate speech research.


Normal and cleft palate anatomy.

Normal and cleft palate anatomy.


Psychological implications of articulation disorders in cleft palate children.


Poor speech following the pharyngeal flap operation; etiology and treatment.


A prospective and randomized series comparing superiorly and inferiorly based posterior pharyngeal flaps.


Invagination of human palatal epithelium prior to contact.


Should the pharyngeal flap be used primarily with palatoplasty?


Studies of dysmorphogenesis in children with oral clefts: 1. Relationship between clinical findings and school performance.


A cephalometric investigation of velar stretch.


The cart before the horse.

The cart before the horse.


Reappearance of murine hypoxanthine guanine phosphoribosyl transferase activity in mouse A9 cells after attempted hybridisation with human cell lines.


Fine structure of fibers in thin sections of condensed chromatin.


Protein synthesis during the cell cycle of L5178Y cells.


On the teaching of dental history.

On the teaching of dental history.


Dental information in libraries.

Dental information in libraries.


Prevention--its role in the curriculum of a new dental school.


Trends in dente.

Trends in dente.


Dental licensing procedures.

Dental licensing procedures.


Unionization.

Unionization.


Third party funder.

Third party funder.


Quoting of fees.

Quoting of fees.


Sterilization of infected root canals. Protests use of article in ADA Journal.


Leukoedema of the oral mucosa. Comments on article.


Socialized medicine.

Socialized medicine.


Fluoridation victory.

Fluoridation victory.


The presence of lead in toothpaste.

The presence of lead in toothpaste.


The Kodak DX 80R replenisher as a quick developer.


The potential of various compounds to suppress microorganisms in plaques produced in vitro by a streptococcus or an actinomycete.


Drug-dependent, alcohol-dependent, and mental patients: clinical study of oral surgery procedures.


Absorbed radiation from panoramic plus bitewing exposures vs full-mouth periapical plus bitewing exposures.


Cost of conducting a dental practice, 1958-1970. II. Bureau of Economic Research and Statistics.


A public health perspective on the limitation of births: report of the State Health Director.


Surgery of the anatomically weak and aging nose.


Mediastinoscopy.

Mediastinoscopy.


Autopsies in Nova Scotia: a legal memorandum.


Citizen advocacy: Canadian experience with a new programme for the habitation of adult retardates.


Adult respiratory distress syndrome.

Adult respiratory distress syndrome.


Treatment of warts.

Treatment of warts.


Observations on the problem oriented medical record.


Tuberculous abscess in the chest wall 25 years after pneumonectomy. Case report to illustrate a slight modification of the problem oriented medical record (Weed system).


Is it ... or isn't it?

Is it ... or isn't it?


Policy statement: Professional Services Committee.


Training--as employees?

Training--as employees?


Guidelines for nurses.

Guidelines for nurses.


Class study: contraception. 2.

Class study: contraception. 2.


Treatment of acute myeloid leukaemia with cytosine arabinoside and daunomycin in combination.


A pathological study on the juvenile type of chronic myeloid leukemia.


First results of routine cytologic diagnosis of uterine cervical carcinoma in one clinic in Israel. Four case reports.


Neuropsychiatric and genetic aspects of a new hereditary disease characterized by progressive dementia and lipomembranous polycystic osteodysplasia.


Grading of transitional cell neoplasms of the urinary bladder from smears of bladder washings. A critical review of 326 tumors.


DNA-content of dysplastic cells of the uterine cervix.


Ethical aspects of the new perspectives in biomedical research.


Biomedical frontiers: genetics.

Biomedical frontiers: genetics.


The brains of suckling mice infected with Junin virus; fractionation and study.


Priorities in preparation for practice. The New York State Nurses Association's 1972 position statement on nursing education.


Two days in a ward.

Two days in a ward.


Herpes simplex encephalitis, cerebrospinal fluid cytology studies. Two case reports.


Tumor cells in blood: the prognostic significance of tumor cells in blood during palpation and biopsy.


The effect of parental death upon children.


Human ovum-like structures in cervical smears.


Cardiogenic shock.

Cardiogenic shock.


Improvement of community health services through the support of indigenous nonprofessionals.


New position descriptions in nursing.

New position descriptions in nursing.


Editorial. Hershey bars and other unhealthy confections.


Cytologic findings indicating pulmonary tuberculosis. II. The occurrence in sputum of epithelioid cells and multinucleated giant cells in pulmonary tuberculosis, chronic non-tuberculous inflammatory lung disease and bronchogenic carcinoma.


A case of primary cavitary Hodgkin's disease of the lungs, diagnosed cytologically.


Hope Medical Center Project Estancia, New Mexico.


Sulfhydryl group catalysis of charge transfer. Sulfhydryl compounds, like cysteine and glutathione, catalyze charge transfer between aldehydes and amines.


Staphylococcal protease: a proteolytic enzyme specific for glutamoyl bonds. An extracellular protease of Staphylococcus aureus, strain V8, previously shown to cleave specifically the peptide bonds on the carboxyl-terminal side of either aspartate or glutamate residues in phosphate buffer (pH 7.8) hydrolyzes only glutamoyl bonds in either ammonium bicarbonate (pH 7.8) or ammonium acetate (pH 4.0). Of all aspartoyl bonds tested, only the Asp-Gly linkage is cleaved at a detectable rate. The staphylococcal protease hydrolyzes all of the seventeen different glutamoyl bonds studied, although those involving hydrophobic aminoacid residues with bulky side chains are cleaved at a lower rate.


The dynamics of life: aging.

The dynamics of life: aging. Biologic aging arises from various causes, including deterioration of cells by mutations, destruction of the cells in organs through infections, aging by failure to eliminate harmful waste products, poisoning from external sources, and by radiation damage. All of these processes lead to equations of the kind observed experimentally for the aging process, so that a proper assessment of the various factors contributing to aging is especially complicated. Many nonbiological processes in which the products of reaction speed up the aging reaction lead to the familiar equation for the rate of aging. Corrosion of metals is exhibited as a typical example of this; many other examples could be given. The general equation for depth of anesthesia brought about by lipid-soluble materials and how this effect can be mimicked by accumulation of waste products is shown to simulate the typical aging curve; it is well known that such waste products are a frequent cause of death.


A comparison of Fe 4 S 4  clusters in high-potential iron protein and in ferredoxin. The structures of both oxidized (HP(ox)) and reduced (HP(red)) high-potential iron protein and of oxidized ferredoxin (Fd(ox)) have been partially refined at 2.0-A resolution by methods similar to those applied to the protein rubredoxin [Watenpaugh, K. D., Sieker, L. C., Herriott, J. R. & Jensen, L. H. (1971) Cold Spring Harbor Symp. Quant. Biol. 36, 359-367]. Average bond lengths and angles in the HP(red) and Fd(ox) Fe(4)S(4) (*) clusters are the same to within the root-mean square (rms) deviation of each mean value. A preliminary comparison of the two HiPIP oxidation states indicates that the HP(ox) cluster is geometrically similar to the HP(red) cluster, but that it is smaller by 0.1-0.2 A in certain dimensions. The HiPIP and ferredoxin cluster geometry is also nearly identical to that reported recently for a synthetic analog [Herskovitz, T., Averill, B. A., Holm, R. A., Ibers, J. A., Phillips, W. D. & Weither, J. F. (1972) Proc. Nat. Acad. Sci. USA 69, 2437-2441]. An apparent paradox presented by the large difference between the HiPIP and ferredoxin electrode potentials can be resolved by the assumption that the Fe(4)S(4) (*) cluster has not two but three oxidation states. The fully oxidized (HP(ox)) and fully reduced (Fd(red)) clusters are paramagnetic, and the intermediate state is spin-paired [Tsibris, J. C. M. and Woody, R. W. (1970) Coord. Chem. Rev. 5, 417-458]. This hypothesis is supported by structural and spectroscopic evidence that the "paired-spin" state exists in both HP(red) and Fd(ox).


Erythropoietin production in cultures of goat renal glomeruli. Cultures initiated with isolated goat-kidney glomeruli produce erythropoietin over periods of 7 months. Production seems dependent upon a minimum interval of about 30 days between changes of medium. Experiments distinguishing between erythropoietin, renal activator, and serum substrate support the conclusion that the factor released to the medium of long-term glomerular cultures is erythropoietin itself. The system offers promising opportunities for studying regulation of erythropoietin production, and possibly could be developed as a source of significant quantities of the hormone.


Ammonia intoxication in rats: protection by N-carbamoyl-L-glutamate plus L-arginine. Rats given a lethal dose (LD(99.9)) of ammonium acetate (10.8 mmol/kg of body weight) were protected to the extent of 85 and 76% when previously injected with N-carbamoyl glutamate or L-arginine, respectively, at a level of 4 mmol/kg of body weight. At a dose of 1 mmol/kg of body weight, L-arginine protected 24%, while N-carbamoyl-L-glutamate protected 61% of the animals. When a combination of N-carbamoyl-L-glutamate plus L-arginine (1 mmol each per kg of body weight) was injected, 100% of the rats were protected. The efficacy of N-carbamoyl-L-glutamate is related to its role as an activator of mitochondrial carbamoyl phosphate synthetase (EC 2.7.2.5) and its resistance to hydrolysis by tissue acylaminoacid acylase. N-Acetyl-L-glutamate, the naturally occurring and most effective activator of mitochondrial carbamoyl phosphate synthetase, was relatively ineffective in protection against lethal dose of ammonium acetate, because of its ready hydrolysis by acylaminoacid acylase. The findings reported provide a rational basis for the use of N-carbamoyl-L-glutamate plus L-arginine in the prevention and treatment of hyperammonemia in clinical conditions of liver disease and parental infusion of amino acids, and in feeding of urea supplements to ruminants.


Mutation of a cytoplasmic gene in Chlamydomonas alters chlorplast ribosome function. A mutation, car, determining resistance to several macrolide antibiotics, including carbomycin, has been identified in the alga Chlamydomonas as cytoplasmic, and mapped in the known cytoplasmic linkage group close to genes determining resistance to other antibiotics, including streptomycin, erythromycin, and spectinomycin. The effect of the car mutation on chloroplast ribosome function was demonstrated with an in vitro system incorporating amino acids especially developed to assess activity of 70S chloroplast ribosomes. In an S-30 extract containing both 70S chloroplast and 80S cytoplasmic ribosomes, low concentrations of Mg(++) and spermidine favored 80S ribosome activity, and high concentrations activated 70S ribosomes and reversibly inactivated the 80S component. Under conditions favoring chloroplast ribosome activity, carbomycin inhibited incorporation by an S-30 extract, and by purified 70S ribosomes from wild-type but not from car cells. These results show that cytoplasmic genes are directly involved in chloroplast ribosome function and they suggest that the car gene product is a ribosomal protein; the results further strengthen the evidence that the cytoplasmic linkage group is located in chloroplast DNA.


Replacement of nerve-growth factor by ganglionic non-neuronal cells for the survival in vitro of dissociated ganglionic neurons. Nerve-growth factor is known to cause a considerable increase in the number of neurons putting out processes and surviving in cell cultures of dissociated dorsal-root and sympathetic ganglia from embryonic chicks. Similar effects of nerve-growth factor have now been noted with cultures of dissociated dorsal-root ganglia from newborn mice or rats. In all three sensory ganglionic systems, the effects of the nerve-growth factor on fiber production and neuronal survival could be mimicked, in the absence of the factor, by adequate increase of the non-neuronal cells in the cultures. The results suggest a hypothesis that views the role of the nerve-growth factor as subordinate to that of the non-neuronal cells.


Formation of bimolecular membranes from lipid monolayers and a study of their electrical properties. Bimolecular membranes are formed from two lipid monolayers at an air-water interface by the apposition of their hydrocarbon chains when an aperture in a Teflon partition separating two aqueous phases is lowered through the interface. Formation of the membrane is monitored by an increase of the electrical capacity, as measured with a voltage clamp. Electrical resistance of the unmodified membrane is analogous to that of conventional planar bilayers (black lipid membranes) prepared in the presence of a hydrocarbon solvent, i.e., 10(6)-10(8) ohm cm(2); the resistance can be lowered to values of 10(3) ohm cm(2) by gramicidin, an antibiotic that modifies the conductance only when the membranes are of biomolecular thickness. In contrast to the resistance, there is a significant difference between the capacity of bilayers made from mono-layers and that of hydrocarbon-containing bilayers made by phase transition; the average values are 0.9 and 0.45 muF cm(-2), respectively. The value of 0.9 muF cm(-2) approximates that of biological membranes. Assuming a dielectric constant of 2.1 for the hydrocarbon region, the dielectric thickness, as calculated from a capacity of 0.9 muF cm(-2), is 22 A. This value is 6-10 A smaller than the actual thickness of the hydrocarbon region of bilayers and cell membranes, as determined by x-ray diffraction. The difference may be due to a limited penetration of water into the hydrocarbon region near the ester groups that would lower the electrical resistance of this region and reduce the dielectric thickness. Asymmetric membranes have been formed by adjoining two lipid monolayers of different chemical composition.


Hydrocortisone induction of rat-liver leucyl-transfer RNA and its synthetases. Within 3 hr after the intraperitoneal administration of hydrocortisone to female rats, a new leucine-accepting tRNA and a new leucyl-tRNA synthetase activity appear in the liver cytosol. The new isoaccepting tRNA can be acylated only with the synthetase derived from livers of hormone-treated animals. Both components are transient; by 12 hr after hydrocortisone administration, the isoaccepting tRNA and its synthetase disappear from livers of treated animals.


Globin messenger RNA activity in erythroid precursor cells and the effect of erythropoietin. Cell populations enriched for erythroid precursor cells were fractionated from 13-day fetal-mouse livers by a method of immune hemolysis. These preparations of precursors, contaminated by less than 7% hemoglobinized erythroblasts, synthesize globin at a rate less than 6% that of the unfractionated liver erythroid cell population. RNA was isolated from these precursor cells and assayed for globin mRNA activity in a cell-free system from Krebs ascites tumor. The 6-16S RNA fraction from precursor cells has less than 5% of the globin mRNA activity of RNA isolated from unfractionated populations. Precursor cells incubated with erythropoietin show an increment in the rate of synthesis of globin only after 5-10 hr of incubation. After 10 hr of culture with this hormone, precursor cells show a 6- to 10-fold increase in globin mRNA activity. These results suggest that the precursor cells of hepatic erythropoiesis, responsive to erythropoietin, do not contain globin mRNA in a biologically active form. Erythropoietin-induced differentiation of these cells to erythroblasts is associated with an increase in globin mRNA.


Human parathyroid hormone: amino-acid sequence of the amino-terminal residues 1-34. Human parathyroid hormone has been isolated in highly purified form from human parathyroid adenomas. The primary sequence of the amino-terminal 34 residues of the human hormone was obtained by automated degradation with a Beckman Sequencer. The phenylthiohydantoin amino acids were identified by gas chromatography and mass spectrometry. The first 34 residues of human parathyroid hormone differ from the bovine hormone by six residues, and from the porcine hormone by five residues. The amino-terminal residue is serine, similar to the porcine parathyroid hormone; bovine parathyroid hormone contains an amino-terminal alanine. Human parathyroid hormone contains two methionine residues, similar to the bovine species, whereas porcine parathyroid hormone contains a single methionine residue. Amino-acid residues in the first 34 that are unique to the human sequence include an asparagine at position 16, glutamine at position 22, lysine at position 28, and a leucine at position 30.


Regulation of aryl hydrocarbon hydroxylase in intraspecific hybrids of human, mouse, and hamster cells. Microsomal aryl hydrocarbon (benzo{a}-pyrene) hydroxylase is an enzyme complex highly inducible by various substrates. The activity of this enzyme complex was studied in hybrid cell strains obtained from parent cells that differ widely in basal as well as in induced enzyme activity. Hybrid cells were made from mouse fibroblasts, 3T3-4C2, with moderate basal and induced enzyme activity and the L-cell subline, A9, which has no detectable hydroxylase activity. Hybrid A9/3T3-4C2 cells exhibited enhanced basal and induced enzyme activities, which were 2- to 5-fold higher than those in 3T3-4C2 cells. In contrast, intraspecific hybrid cells of the human cell lines, JEC-3 and VA-2, and the hamster cell lines, OBP and BHK, exhibited a reduced basal and induced enzyme activity that was similar to or lower than that of the parent with low enzyme activity. In all hybrid cells examined, an association was observed between basal and induced enzyme activities, suggesting a closely coupled regulation of these activities.


An enhancing role for DNA synthesis in formation of bacteriophage lambda recombinants. Recombination in some intervals of the map of phage lambda is associated with more DNA synthesis than in other intervals. Blockage of DNA synthesis by high temperature in a host temperature-sensitive for DNA synthesis results in the relative reduction of recombinant frequencies in those regions having the larger amounts of recombination-associated synthesis. Reduction of DNA synthesis at normal temperatures by a combination of the bacterial mutation and a mutation in one of the phage genes required for DNA synthesis has the same consequence. Therefore, DNA synthesis enhances recombinant particle formation more in some map intervals than in others.


Positive control of endolysin synthesis in vitro by the gene N protein of phage lambda. Positive control in vitro by gene N protein of bacteriophage lambda was demonstrated. lambda DNA was used to direct in vitro synthesis of lambda endolysin in a cell-free protein-synthesizing preparation derived from Escherichia coli. The endolysin synthesis depends on the concomitant in vitro synthesis of lambda gene N protein. When lambda N(-) DNA was used to direct the cell-free preparation, endolysin was made only if extract was added from cells in which a lambda prophage had been induced. The use of various prophage deletion strains proved that if this stimulating activity made in vivo is coded by a known lambda gene, it must be coded by gene N. The ability to stimulate endolysin synthesis in vitro on a lambda N(-) DNA template, therefore, constitutes an assay for N protein.


The evolutionary development of modifier genes. The main findings of a study of the evolution of modifier gene frequencies in models of deterministic population genetics are presented. A wide variety of random mating systems are subject to selection with modifiers operating, in different cases, on mutation rates, dominance, migration between subpopulations, and linkage between other loci. In all these instances the modifier frequencies evolve in such a way as to maximize the mean fitness of the population at equilibrium. This is remarkable since, except for the dominance modifier, the modifier genes are selectively neutral in the sense that they do not affect the fitness of their individual carriers. In nonrandom mating systems the mean fitness concept is not well defined, and there does not appear to be such a simple principle governing the evolution of modifier frequencies. In assortative mating systems modifiers favoring reduced assortment propensities tend to increase. In contrast, for selfing-outcrossing systems modifiers favoring increased selfing tend to increase.


Globin messenger-RNA induction during erythroid differentiation of cultured leukemia cells. A cloned line of murine proerythroblastoid cells (T-3-Cl-2), transformed by Friend leukemia virus, undergoes changes associated with erythroid differentiation when treated with dimethylsulfoxide in culture. This line, which does not undergo spontaneous differentiation, develops specific erythrocyte-membrane antigen and accumulates detectable amounts of heme within four days of dimethylsulfoxide treatment. In the present study, we have followed the phenotypic expression of the globin genes by measuring globin mRNA in differentiating cells. Our hybridization probe for this purpose is [(3)H]DNA, which is complementary to purified globin mRNA, synthesized by viral RNA-directed DNA polymerase. This probe is sufficiently sensitive to detect less than 1 ng of globin mRNA. Using it, we find little or no hybridizable globin mRNA in either uninduced cells or in treated control lymphoid cells. In contrast, globin mRNA can be detected in T-3-Cl-2 cell 2 days after induction by dimethylsulfoxide; it reaches a maximum concentration four days after induction. At this time, cells that stain positively for heme appear. The hybridizable cytoplasmic RNA induced in these cells has the sedimentation properties of 9S globin mRNA. Considering the stable character of globin mRNA, our results are most readily explained in terms of a transcriptional activation of the globin genes.


Conformational energy studies of oxytocin and its cyclic moiety. Conformational energies were calculated for oxytocin in water, starting with a conformation proposed from nuclear magnetic resonance measurements in [U-(2)H](CH(3))(2)SO. Calculations on the isolated ring showed that conformations with one transannular hydrogen bond had the same energies as those without such bonds; those with two such hydrogen bonds do not appear to form. Calculations on the whole molecule also indicated the existence of several low-energy minima in the energy surface, and no preference for hydrogen-bond formation in the cyclic moiety; the hydrogen bond proposed between the Gly peptide NH and the Cys-6 C=O in the acyclic moiety can form. The proposed proximity of the tail to the ring is one of two low-energy conformations found. The Tyr side chain had two conformations of comparable energy, one over the ring between the Gln and Asn side chains, and the other with the Tyr side chain away from the ring. The oxytocin molecule appears to be flexible, and is probably sensitive to changes in its environment.


Partial characterization of a tetrodotoxin-binding component from nerve membrane. Tetrodotoxin from Japanese puffer fish has been labeled with tritium and purified from the crude mixture obtained. The interaction between the purified [(3)H]tetrodotoxin and membrane suspensions from the olfactory nerve of long-nosed garfish has been investigated by equilibrium dialysis. Tetrodotoxin binds to membrane suspensions with a dissociation constant K(D) = 8.3 nM. The nerve preparation binds 42 pmol of [(3)H]tetrodotoxin/g of wet tissue at saturating toxin concentrations. With various hydrolytic enzymes, the binding component is shown to be a protein embedded in a phospholipid environment. The binding is inhibited below pH 4.0 and is not stable towards heat. Tetrodotoxin binding is not inhibited by the local anesthetic, procaine.