A diffusible factor restoring contact inhibition of growth to malignant melanocytes. Contact inhibition of growth is the property in vitro whose loss is most closely correlated with tumorigenicity in vivo. A contact-inhibited melanocytic cell line produces a diffusible protein-containing factor capable of restoring contact inhibition of cell division to highly malignant hamster melanocytes. Its addition to subconfluent cultures of the malignant cells is followed by the obligatory acquisition, at confluence, of the contact-inhibited state. Cultures are flat, oriented, and fibroblast-like, and show a 55% decrease in saturation density with no loss of viability. The effect is reversible. Present in conditioned media of cultures of the contact-inhibited melanocytic cell line, isolated by column chromatography on Sephadex G-200, the factor appears to be of high molecular weight. Activity is preserved in aqueous solutions at 4 degrees for at least 8 weeks, but is destroyed by repeated freezethawing or by treatment with Pronase. This newly recognized contact-inhibitory factor may be a prototype for a more general and fundamental mechanism for regulation of normal cell-cell interactions. It is the first cell-elaborated factor to be isolated that is capable of restoring the capacity for contact inhibition of growth to malignant cells.


Globoid cell leukodystrophy: deficiency of lactosyl ceramide beta-galactosidase. Activity of lactosyl ceramide beta-galactosidase (beta-D-galactoside galactohydrolase, EC 3.2.1.23) was found to be extremely low in enzyme preparations from liver, brain, and cultured skin fibroblasts from patients with Krabbe's disease. Leukocytes from one set of parents had enzyme levels approximately half those measured in control leukocytes. The low activity observed for this galactolipid hydrolase is the fourth enzymatic deficiency noted for this genetic disease. Beta-galactosidase activity toward galactocerebroside, psychosine, and monogalactosyl diglyceride is also low in patients with Krabbe's disease. Other lysosomal enzymes measured were found to be in the normal range. This enzymatic defect may provide a better explanation for the pathological and chemical findings previously reported for this syndrome.


Comparison of human serum dopamine-beta-hydroxylase levels by radioimmunoassay and enzymatic assay. Human serum dopamine-beta-hydroxylase (3,4-dihydroxyphenylethylamine, ascorbate:oxygen oxidoreductase, EC 1.14.17.1) has been measured in a normal adult population by both enzymatic assay and by radioimmunoassay. The levels of enzymatic activity varied over a very wide range from 0.6 units/ml to 85 units/ml. In contrast, the amount of total dopamine-beta-hydroxylase protein present in these samples, as measured by radioimmunoassay, varied only over a 3-fold range: from 11.7 mug/ml to 34.4 mug/ml. From an analysis of the purified enzyme, the percentage of serum dopamine-beta-hydroxylase that was catalytically active was estimated to be small and variable.


Migration enhancement factor: a new lymphokine. Production of human migration inhibitory factor by lymphocytes exposed to antigen was studied at intervals over a 7-day period. Migration inhibitory factor was measured by an agarose gel method, with buffycoat leukocytes as indicator cells. Lymphocyte supernatants from 7-day cultures consistently showed migration inhibitory factor activity; by contrast, enhancement of migration was frequently noted when effector cells were exposed to supernatants from 2- to 5-day cultures. Enhancement activity was manifested either by enhanced migration or by a sequential reduction in inhibitory activity consistent with a factor opposing the action of migration inhibitory factor. When supernatants were subjected to polyacrylamide gel electrophoresis, enhancement activity was regularly found in the beta-globulin region and migration inhibitory factor in the albumin fraction of the gel. The enhancement activity was heat-stable and nondialyzable. These findings characterize a hitherto unreported lymphokine, migration enhancement factor.


Evolution and the distribution of glutaminyl and asparaginyl residues in proteins. Recent experiments on the deamidation of glutaminyl and asparaginyl residues in peptides and proteins support the hypothesis that these residues may serve as molecular clocks that control biological processes. A hypothesis is now offered that suggests that these molecular clocks are set by rejection or accumulation of appropriate sequences of residues including a glutaminyl or asparaginyl residue during evolution.


The membrane junctions in communicating and noncommunicating cells, their hybrids, and segregants. Human Lesch-Nyhan cells, which are coupling and have gap junctions, were fused with mouse cl-lD cells, which are noncoupling and lack gap junctions. The resulting hybrid cells were coupling and had gap junctions while they contained the nearly complete complement of parent chromosomes. As the hybrid cells lost human chromosomes, clones appeared among the segregants, which had reverted to the noncoupling and junction-deficient trait of the mouse parent cell. The human cell appears to contribute a genetic factor to the hybrids that corrects the junctional deficiency of the mouse cell.


Ethological isolation among three species of the planitibia subgroup of Hawaiian Drosophila. Drosophila heteroneura and D. silvestris are sympatric species living on the island of Hawaii, while D. planitibia is allopatric on the nearby island of Maui. A pronounced ethological isolation is found between the sympatric species, and none between allopatric ones, except that D. planitibia females discriminate against D. heteroneura males. Male hybrids are sterile in allopatric crosses but fertile in sympatric ones. The ethological isolation and the hybrid sterility are uncorrelated. This is expected if premating isolation between closely related species is an ad hoc product of natural selection, while postmating isolation is an incidental result of genetic divergence. Some exceptions to the rule are discussed.


Galactosyltransferase and concanavalin A agglutination of cells. A correlation has been observed between concanavalin A agglutination of various cell types and the presence of surface membrane galactosyltransferase (1-O-alpha-D-Galactosyl-myo-inositol:raffinose galactosyltransferase, EC 2.4.1.67) activity. Moreover, a reduction to less than 50% of cell surface galactosyltransferase activity occurred after treatment with concanavalin A; other cell surface glycosyltransferase enzyme activities examined were unaffected by concanavalin A treatment. To confirm the participation of cell surface galactosyltransferase in concanavalin A-induced cell agglutination, the enzyme from rabbit erythrocytes was solubilized by sonication and purified by preparative polyacrylamide gel electrophoresis. It was possible to achieve a purified preparation of rabbit erythrocyte galactosyltransferase by separation on concanavalin A-Sepharose. The purified enzyme showed visible immunoprecipitation (Ouchterlony) with concanavalin A. Furthermore, human erythrocytes, which are not normally agglutinated by concanavalin A, became agglutinable by the lectin when the erythrocytes were preincubated with purified galactosyltransferase. These experiments suggest a direct and possible specific role of cell surface galactosyltransferase enzyme in the mechanism of concanavalin A agglutination of cells.


Concordance of collagen-based radiocarbon and aspartic-acid racemization ages. By determining the extent of racemization of aspartic acid in a well-dated bone, it is possible to calculate the in situ first-order rate constant for the interconversion of the L and D enantiomers of aspartic acid. Collagen-based radiocarbon-dated bones are shown to be suitable samples for use in "calibrating" the racemization reaction. Once the aspartic-acid racemization reaction has been "calibrated" for a site, the reaction can be used to date other bones from the deposit. Ages deduced by this method are in good agreement with radiocarbon ages. These results provide evidence that the aspartic-acid racemization reaction is an important chronological tool for dating bones either too old or too small for radiocarbon dating. As an example of the potential application of the technique for dating fossil man, a piece of Rhodesian Man from Broken Hill, Zambia, was analyzed and tentatively assigned an age of about 110,000 years.


Isolation and analysis of somatic hybrids derived from two human diploid cells. Lesch-Nyhan fibroblasts and normal human leukocytes with different glucose-6-phosphate dehydrogenase genotypes were fused by Sendai virus. Clones were isolated on the basis of their resistance to a medium containing hypoxanthine, amethopterin, and thymidine and ability to proliferate in monolayer culture. These mononuclear cells (1) incorporated [(3)H]hypoxanthine, (2) expressed the glucose-6-phosphate dehydrogenase heteropolymer, and (3) were polyploid. Therefore, hybrids can originate from the fusion of two diploid human cells. X chromosome inactivation did not occur in these hybrid cells of male origin. The hybrids expressed both parental genomes and exhibited the senescence and contact feeding characteristic of the human skin fibroblast.


Genetic recombination in Rhodopseudomonas capsulata. A system for genetic exchange in Rhodopseudomonas capsulata has been discovered. Each genetic marker thus far examined can be transferred, and many strains of Rps. capsulata can participate in genetic exchange. The mechanism of gene transfer seems unlike that of any previously described bacterial system, since genes can be transferred by cell-free filtrates, but the vector is resistant to deoxyribonuclease and has a sedimentation constant of about 70 S.


Studies on anal function in continent and incontinent patients.


Photochemical attachment of lac repressor to bromodeoxyuridine-substituted lac operator by ultraviolet radiation. The transducing phage lambdah80dlac carries the lac operator, whereas wild-type lambdah80 does not. We find that in high salt (0.18 M KCl), ultraviolet radiation causes the formation of a very stable complex between repressor and 5-bromodeoxyuridine (BrdU)-substituted lambdah80dlac but not to BrdU-lambdah80 DNA. Studies with inducers of the lac operon confirm the specificity of attachment. In low slat (0.01 M KCl), ultraviolet radiation will also attach repressor nonspecifically to BrdU-lambdah80 DNA. The stability of the complex suggests that covalent bonds are formed. We also report that another regulatory protein, the catabolite gene activator protein, can be attached similarly to DNA.


Structure of nascent phiX174 replicative form: evidence for discontinuous DNA replication. varphiX174 RF (replicative form) II DNA, labeled in vivo with [methyl-(3)H]thymidine, was isolated from Escherichia coli polA (DNA polymerase I-deficient) and polA(+) cells during RF replication. [(32)P]dCMP was incorporated into the gaps present in the RF II DNA with [alpha-(32)P]dCTP and T4 DNA polymerase. Sedimentation in alkaline sucrose gradients revealed that much of the incorporated (32)P was present in a heterogeneous collection of fragments shorter than unit length. Inclusion of polynucleotide ligase in the gap-filling reaction increased the average size of the (32)P-labeled fragments. Gel electrophoresis of the products formed by digestion of the (32)P-labeled RF II molecules with the restriction nuclease, endonuclease R, indicated that in the population of RF II molecules gaps could occur anywhere in the genome. Competition-annealing experiments provided evidence that the majority of the label incorporated into gaps was present in the minus strand. RF II molecules isolated from polA(+) cells were enriched for gaps in a unique region of the genome in comparison with RF II molecules isolated from polA cells. The presence of multiple gaps in the minus strand implies that it is synthesized by a discontinuous mechanism during varphiX RF replication.


Increased caries-incidence by oral inoculation of cariogenic bacteria in rats after dietary fluoride.


A morphological study of the influences of fluoride on rat molar teeth.


A chemical catalytic method for determination of silver in human saliva.


Effects of hydrocortisone on the development in vitro of the secondary palate in two inbred strains of mice.


Effects of dental plaque on the production and release of lysosomal hydrolases by macrophages in culture.


The effects of propanol, butanol, chlorhexidine, fluoride and combinations on the potassium and phosphate translocation and acid production by Streptococcus mutans.


Human dental plaque: stimulation of bone resorption in tissue culture.


The interaction of human parotid salivary proteins with hydroxyapatite.


The effect upon dental caries incidence in rats of varying the cage conditions and the maternal diet before weaning.


The isolation from human parotid saliva of a tyrosine-rich acidic peptide which exhibits high affinity for hydroxyapatite surfaces.


Effect of increased mastication on the secretory process of the rat parotid gland.


Treating acute leukaemia in special centres.


The geographic variation of the incidence of malignant primary bone tumors in Sweden.


Giant-cell tumors of bone in South India.


Effects of caffeine and theophylline on DNA synthesis in unirradiated and UV-irradiated mammalian cells.


Autopsy rates in large New Zealand hospitals.


Parents as therapists with a severely atypical child.


Lactic acidosis associated with phenformin therapy in diabetes mellitus.


Spontaneous rupture of tendons in rheumatoid arthritis.


Spinal thrombophlebitis after prostatectomy with hypotensive anaesthesia: case report.


The course of the palatine arteries during secondary palate development in the rat.


Pesticides, health and ecology.

Pesticides, health and ecology.


Editorial: The position of paraplegics in New Zealand.


Editorial: Flower-borne infections.

Editorial: Flower-borne infections.


Urinary excretion of C6-C10 dicarboxylic acids in glycogen storage disease types I and 3.


Comparison of photoperiodic influences on the seasonal changes in body weight and energy intake in the eastern chipmunk and the 13-lined ground squirrel.


New developments in clinical psychology.


Ultrastructure of muscle in stiff-man syndrome.


Multiple myeloma superimposed on chronic myelocytic leukemia. A 65-year-old woman with chronic myelocytic leukemia and multiple myeloma is described. Cases of acute leukemia complicating multiple myeloma have been reported in recent years, but to our knowledge this is the first case where multiple myeloma developed in a patient who had pre-existing chronic myelocytic leukemia.


Mandibular dimensional change.

Mandibular dimensional change.


Systemic factors in alveolar bone loss.

Systemic factors in alveolar bone loss.


Skin-surface reference point for locating the condyle with teeth in centric occlusion.


Pin-retained composite resin cores for posterior teeth.


An onlay technique for teeth in supraversion.


Transfer base for removeable partial dentures.


Letter: "Aseptic meningoencephalopathy" or "neurotoxicity" in leukemia?


Cancer cachexia and gluconeogenesis.

Cancer cachexia and gluconeogenesis.


Paraneoplastic syndromes. Hyperglycemia.


Paraneoplastic syndromes. Hyper- and hypocalcemia: pathogenesis and treatments.


Neuroblastoma-pheochromocytoma: products and pathogenesis.


Feminization, virilization, and precocious sexual development that results from neoplastic processes.


Anemia of cancer.

Anemia of cancer.


Granulocytosis in neoplasia.

Granulocytosis in neoplasia.


Altered coagulability in tumor patients: introduction.


Hemostasis in malignancy.

Hemostasis in malignancy.


Tumors, mucus production, and hypercoagulability.


Bleeding and intravascular clotting in malignancy: a review.


Endocrinologically active mesenchymal tumors.


A spectrum of organ systems that respond to the presence of cancer. Abnormalities of taste as a remote effect of a neoplasm.


A spectrum of organ systems that respond to cancer: the joints and connective tissue.


A spectrum of organ systems that respond to the presence of cancer. How and why the skin reacts.


Paraneoplastic syndromes. Other causes, diagnosis, and treatment of other syndromes. General discussion.


Newer concepts in pathogenesis. Secondary endocrine and mammary malignancies as main signs of hormonal syndromes produced by endocrine tumors.


A classification of ectopic hormone-producing tumors.


Paraneoplastic syndromes in experimental animal model systems.


Oncocognitive autoimmunity and other paraneoplastic syndromes yet to be described.


Generalized perturbations in host physiology caused by localized tumors. Tumors and fever.


How tumors affect host metabolism.

How tumors affect host metabolism.


Lactic acidosis in malignancy and observations on its possible pathogenesis.


Discussion paper: dissipative and nondissipative structure.


Discussion paper: rhythmicity of fundamental biologic phenomena.


A mathematical model for catatonic schizophrenia.


Discussion paper: the qualitative analysis of partially specified systems.


Discussion paper: recent progress in stagraphics.


Mathematical analysis of fundamental biological phenomena. General discussion.


Discussion paper: a geometry for biology.


A catastrophe theory of evolution.

A catastrophe theory of evolution.


Mathematical analysis of fundamental biological phenomena. Opening remarks.


The role of plasticity in biological and cultural evolution.


Discussion paper: growth, form, and function in mammals.


Nonequilibrium problems in biological phenomena.


Phenomena of multiplicity, stability, and symmetry.


Biochemical and haematological findings in malignant bone tumours.


Amantadine may stimulate dopamine and noradrenaline receptors.


Transplantation and tissue culture studies of radiation-induced osteosarcoma in the rat.


Pseudosarcomatous fasciitis.

Pseudosarcomatous fasciitis.


Antifilarial activity of levamisole hydrochloride against subperiodic Brugia malayi infection of domestic cats.


The cryopreservation of colony-forming cells from the bone marrow of children with acute-lymphocytic leukemia.


Inhibition of nucleoside transport by aflatoxins and sterigmatocystin.


L-threonine deaminase in marine planktonic algae. IV. Studies of substrate specificity and inhibition from substrate analogs. Evidence for additional L-serine deaminase activity in the cryptophyte Chroomonas salina.


Rosette formation of human erythrocytes on cultured cells of tumour origin and activation of complement by cell membrane.


Editorial: The status of universal licensure.


Cytogenetic studies in thirteen patients with pancytopenia and leukaemia associated with long-term exposure to benzene.


National health legislation and dentistry.


Of Jello, dental students and practitioners.


'Dead Tooth' is a misnomer.

'Dead Tooth' is a misnomer.


Kids may have alternative to those ugly teeth braces.


Preventive dentistry should start during pregnancy.


Mouth guards prevent most oral injuries in contact sports.


Do mental disorders cause dental disorders?


The regulation of myelopoiesis as approached with in vivo and in vitro techniques.


Acquired disorders of hemoglobin.

Acquired disorders of hemoglobin.


Cell kinetics of orthodontically-stimulated and non-stimulated periodontal ligament in the rat.


A method of studying patterns of tooth displacement in simulated chewing cycles in man.


Time sequence of the activity of the temporal and masseter muscles in healthy young human adults during habitual chewing of different test foods.


The effects of stimulus polarity and electrode area on the threshold to monopolar stimulation of teeth in human subjects, with some preliminary observations on the use of a bipolar pulp tester.


131I and 99mTc uptake by the thyroid and submaxillary glands in male and female A2G mice.


Circadian variations in human resting submandibular saliva flow rate and composition.


Submandibular secretion in the dog after intraluminal injections of amiloride.


The mechanism of vocalization in the marsupial Didelphis azarae.


Rabbit palatal mucosa sialoglycoproteins: solubilization and biosynthesis in vitro.


Electron microprobe analysis of iron content of incisor enamel in some species of Rodentia.


Feline dental pulp photoplethysmography during stimulation of vasomotor nerve supply.


Isolation and classification of anaerobic bacteria from intact pulp chambers of non-vital teeth in man.


Neutrophil chemotactic activity of rabbit neutrophils.


Editorial: Research in psychiatry.

Editorial: Research in psychiatry.


Proceedings: Adolescents--are they normal?


Proceedings: Conflict at the interface or the Janus effect.


Proceedings: A child is being beaten.

Proceedings: A child is being beaten.


Proceedings: Techniques of family therapy of adolescents.


Proceedings: Adolescent psychiatry and the concepts of "open system" in the service of ego growth and evolvement.


Proceedings: Adolescents who kill parents--reactive parricide.


Proceedings: Patient response to psychiatric consultation in a general hospital.


Proceedings: Cultural conflict and transition: epidemic hysteria and social sanction.


Letter: Doctors who suicide.

Letter: Doctors who suicide.


Letter: Hypertension and depression.

Letter: Hypertension and depression.


Venereal diseases in the Wellington Hospital Women's Clinic 1971.


Growth hormone in dystrophia myotonica.

Growth hormone in dystrophia myotonica.


The day ward--Cornwall Geriatric Hospital.


Exacerbation of nephrolithiasis by a carbonic anhydrase inhibitor.


Pancreatic ascites: case report.

Pancreatic ascites: case report.


Staphylococcus epidermidis as a cause of liver abscess: case report.


Pancreatitis with hyperlipaemic serum occurring with hypogonadism and congential blindness.


Diet, habits, and living conditions of "world travellers" in Nepal.


Aspects of community health teaching in a medical school.


Editorial: Drug misuse.

Editorial: Drug misuse.


Editorial: Management of status epilepticus.


An obstetric list.

An obstetric list.


Letter: Isolation of intestinal parasites.


Asynchronisms between DNA and RNA synthesis in leukemic cells.


Effect of chlorhexidine mouthrinse and periodontal treatment upon bacteremia produced by oral hygiene procedures.


Mineral content of developing rat incisor enamel.


Incorporation of iron in rat incisor enamel.


On the mechanism of cytotoxicity of silver and copper amalgams in a cell culture system.


Assessment of a preventive dentistry programme in private practice.


Editorial: Private practice in a public health scheme--strengths and difficulties.


Cell rests of the sheep periodontium.

Cell rests of the sheep periodontium.


Editorial: Dental treatment of the aged.


Dental aspects of cardiac abnormality.

Dental aspects of cardiac abnormality.


Alcoholism: the drug problem of the 1970's.


Alcohol and the nervous system.

Alcohol and the nervous system.


Alcohol and the law.

Alcohol and the law.


Aspergillosis in Auckland.

Aspergillosis in Auckland.


A review of the isolation of intestinal parasites at Green Lane Hospital 1971-1972.


Editorial: Some protective measures in road traffic accidents.


Letter: Tooth injuries.

Letter: Tooth injuries.


Oral hygiene. 1.

Oral hygiene. 1.


The D.S.A. and the sub-normal patient.

The D.S.A. and the sub-normal patient.


Symposium on the early treatment of the cleft lip and palate: the present dilemma.


The influence of pre-surgical orthodontic treatment upon the surgery of cleft lip and palate.


An evaluation of pre-surgical treatment.


Autogenous tooth transplantation: a clinical and histological investigation.


Extra-oral traction and functional appliances--a cephalometric comparison.


A thousand consecutive treated orthodontic cases--a survey.


The size of the human tongue shadow in different mandibular postures.


A thumb sucking deterrent.

A thumb sucking deterrent.


A dental extraction performed under acupuncture.


Deciduous occlusion in children with repaired complete clefts of the lip and palate.


Forensic aspects of dental science.

Forensic aspects of dental science.


Fluoridation.

Fluoridation.


Viral hepatitis and the dental surgeon.

Viral hepatitis and the dental surgeon.


Fluorides and dental health.

Fluorides and dental health.


Some current treatment procedures for cleft lip and cleft palate.


A unique dental shop.

A unique dental shop.


Case report: maxillary subperiosteal implants.


'Progressive relaxation' eases masticatory pain syndrome.


Conservative technics to prevent, treat atraumatic cusp fractures.


The dental industry: 'forgotten' workhorse of the dental team.


'People place' emphasizes prevention.

'People place' emphasizes prevention.


Dental studies yield clues to collapse of ancient Mayan empire.


From 'old' to new on a modest budget.

From 'old' to new on a modest budget.


How to control the 'runaway practice'.

How to control the 'runaway practice'.


The gerbil as a model for the study of the epilepsies. Seizure patterns and ontogenesis.


Epileptogenic properties of cobalt and related metals applied directly to cerebral cortex of rat.


Taxometric classification of myoclonic epilepsies.


The hippocampal-dentate complex in temporal lobe epilepsy. A Golgi study.


Epilepsia partialis continua with well-delimited subcortical frontal tumor.


Pedunculated lesions--part 2 (smooth surface).


A simple process for duplicating radiographs.


My concept on occlusion. Aspects on clinical evaluation with gothic arch tracing in edentulous cases.


Successful indentification of a bite mark in a sandwich.


Some aspects of the chemical composition of translucent dentine.


Identification and tooth morphology.

Identification and tooth morphology.


New guidelines for dental recording.

New guidelines for dental recording.


Problems associated with the treatment of hyperplastic gingivitis.


Fluoride contents of water supply in different regions in Irag.


Full mouth rehabilitation. A case report.


An assessment of gold caps "sharp crowns".


The orthopantomograph.

The orthopantomograph.


Psychology and mouth rehabilitation.

Psychology and mouth rehabilitation.


Burstone auxiliary cuspid tube.

Burstone auxiliary cuspid tube.


Orthodontic office planning.

Orthodontic office planning.


New removable mandibular anterior retainers. 1. Acrylic and metal. 2. All-metal.


An accurate indirect archwire technique.


Cuspid retraction with the vertical spur.


Accurate prefabrication of multilooped archwires.


Phase IV.

Phase IV.


Clinical aids. Relieving trauma from an inner headgear bow.


Orthodontics in Turkey.

Orthodontics in Turkey.


Management of endodontically inaccessible root canals in mandibular posterior teeth.


Chondroma of the mandible with early malignant changes.


Surgical management of ankylosis of temporomandibular joint.


A rare case of accessory duct in sub-mandibular sialography.


Effect of a school-based dental disease prevention program on a socio-economically deprived population.


Oral-antral fistulas: a review.

Oral-antral fistulas: a review.


Prevention multiphasic testing versus the hot pursuit of plaque.


Flossing: the great American hoax?

Flossing: the great American hoax?


Occlusal harmony in restorative dentistry.


Tetracycline antibiotics and tooth discoloration.


Porcelain inlays utilizing retentive pins.


A new look at insurance.

A new look at insurance.


Anterior balancing interferences.

Anterior balancing interferences.


Resume of potential preventive benefits from a fluoride-containing prophylactic cup.


Initial preparation.

Initial preparation.


A simplified technique for immediate stabilization of implants in experimental animals.


The use of bladevent implants in a selected population of partially edentulous adults: a three year report.


Intramucosal inserts: conserve edentulous ridges and increase retention and stability of removable maxillary prostheses.


Occlusion and the endosseous implant.

Occlusion and the endosseous implant.


A case report of a two year blade vent implant.


The influence of remodelling on mandibular bone structure.


Hemidesmosomes and the adhesion of junctional epithelial cells to metal implants--a preliminary report.


Endodontic-endosteal implants: biologic considerations.


Implant registration program.

Implant registration program.


The implant sulcus.

The implant sulcus.


Mucosal inserts solve gagging problem.

Mucosal inserts solve gagging problem.


Preventive dentistry concepts for endosseous implantology.


Tissue response to functioning dental implants.


The dentiscope system of plaque control.


Auxiliaries--time to call a halt.

Auxiliaries--time to call a halt.


Examining the toxic dose. 3.

Examining the toxic dose. 3.


False aneurysm of the mandible.

False aneurysm of the mandible.


Clinical observations on composite resin placement--a preliminary report.


Environmental hazards of anaesthesia.

Environmental hazards of anaesthesia.


Credit where credit is not due.

Credit where credit is not due.


The humane approach to the patient.

The humane approach to the patient.


Recognition and treatment of gingival recession.


Recent advances in dental restorative materials.


Andrews Bridge Construction for a haemophiliac patient.


Towards aspiration and safer local anaesthesia in dentistry.


Foods and drinks that will cause you the fewest cavities.


Hetacillin (Versapenr)--a new (?) antibiotic.


Dentistry and consumerism.

Dentistry and consumerism.


"What is a "prophy"?

"What is a "prophy"?


Editorial: Deadly sucrose.

Editorial: Deadly sucrose.


Toward better dentistry: the 1973 continuing education survey.


Don't ever underestimate the importance of your telephone.


A new hospital for Meharry Medical College.


Fluoride in teeth of rats using citrus beverage.


Bone physiology and periodontal disease.


NOgenol bite registration paste.

NOgenol bite registration paste.


A dental laboratory technology program at the high-school level.


A camera for contact microradiography.

A camera for contact microradiography.


Sialography with Telebrix 38. A comparative study with Lipiodil-UF in the diagnosis of salivary gland diseases.


Progressive maxillofacial osteolysis. A case report.


Pathogenesis and x-ray diagnostics of lateral fractures of the middle third of the facial skeleton.


Measurement of x-ray exposure of ocular lens in dento-maxillo-facial radiography.


Comparative study between monochrome color roentgen film and standard black-and-white film.


Pendunculated lesions. 3. "Pebbled" variant.


"Tooth--supported complete dentures for the quadriplegic". A case report.


The national health service reorganisation and the role of the general dental practitioner in Scotland.


Chronic myelogenous leukemia presenting as gastric polyps: diagnosis by endoscopic polypectomy.


Application of videofluorography in prosthetic dentistry. A preliminary report.


Microbiology in Glasgow Dental School.

Microbiology in Glasgow Dental School.


Glasgow Dental Hospital and School--a utilisation study.


The dental materials science labortory.

The dental materials science labortory.


Expanded role for the forensic dentist: sexing skeletal remains.


The dentist's role in the detection of laryngeal and nasopharyngeal lesions: prevention of morbidity.


Prevention--a personal opinion.

Prevention--a personal opinion.


Why we are failing with oral cancer.

Why we are failing with oral cancer.


Quality: a student evaluation of prevention.


The dentist and detection of skin cancer of the head and neck.


Dental x-ray protection.

Dental x-ray protection.


A fluorescent microscopic study of human bacterial plaque smears stained with the plak-lite fluorochrome.


The status of the dentist in the hospital and the community. Responsibility of the hospital to multiphasic community dental health care.


Knowing how is not enough: tell them why.


Community dental health education for dental hygienists.


The principles underlying the role of refined carbohydrate in the incidence of dental disease.


The role of dental identification in mass disasters.


The role of the dentist in the criminal field--case report.


Dental materials--a short guide for the general practitioner. 2. Linings and cements.


Consultant's views on review.

Consultant's views on review.


TV's effect on nutrition.

TV's effect on nutrition.


Honoraria--fees for clinicians and essayists.


A review of dental cements used for permanent retention of restorations. I. Composition and manipulation.


Kalamazoo public schools dental research project.


Zinc-oxide eugenol as a pulp capping agent.


Differential diagnosis of pain syndromes relating to the temporomandibular joint.


A system for patient referrals.

A system for patient referrals.


Accuracy of casts produced from two polyether rubber elastomers.


(House MM),+HOUSE MM:The M. M. House Memorial Museum.


Inside dental research. The neuroanatomy of maxillofacial pain.


What happens to your amalgam restorations?


Editorial: The malpractice dilemma.

Editorial: The malpractice dilemma.


Sweet peas, copper, pregnancy, and pills: effects on aorta.


Forewarnings of major subarachnoid hemorrhage due to congenital berry aneurysm.


Diabetic coma: guidelines in therapy.

Diabetic coma: guidelines in therapy.


Metastatic testicular carcinoma: single-agent chemotherapy (actinomycin D) in treatment.


Lithium carbonate: psychiatric indications and medical complications.


Cardiac structural and functional changes after myocardial infarction. I. Dyssynergy.


Treating people with peptic ulcers.

Treating people with peptic ulcers.


Chlorpromazine causing extrapyramidal dysfunction in newborn infant of psychotic mother.


Intravascular hemolysis with traumatic arteriovenous fistula: another cause of microangiopathic hemolytic anemia.


United way medical research and training. What are we gettin for our money?


Comprehensive alcoholism program in New York City: a year's experience.


Sexual problems as seen by proctologist.


Space maintenance in the primary and mixed dentitions.


Hard tissue surgery.

Hard tissue surgery.


Cysts of the jaws.

Cysts of the jaws.


A dental viewpoint of drug interactions. I.


Changes in occlusion and craniofacial pattern between 11 and 25 years of age. A follow-up study of individuals with normal occlusion.


Growth changes in cases of mandibular micrognatha.


Changes of the soft tissue profile during growth.


Changes in profile in complete bilateral cleft lip and palate from infancy to adolescence.


Cranio-facial pattern in boys with unilateral complete clefts at 10-12 years of age.


A comparative study of treatment and occlusion in unilateral cleft palate subjects.


Effects of adenoidectomy on dentition and nasopharynx.


The distribution of 65Zn in the nasal cartilage and the effect of zinc deficiency on the endochondral growth of the cranial base.


Changes in craniofacial pattern caused by functional adaptation--an experimental study in young rabbits.


Growth of the facial skeleton of the rate studied by vital staining with bone marking dyes.


Orthodontic therapy an exercise in decision making.


A cephalometric follow-up study of overjet and incisor stability in treated class II, division 1.


A stability of the lower dental arch after orthodontic treatment.


A longitudinal clinical and cephalometric study of 50 malocclusion cases treated with activator appliances.


Effects of activator therapy on the craniofacial skeleton in class II, division 1 malocclusion.


Indications for and working action of bimaxillary appliances.


Some changes in occlusion and craniofacial pattern obtained during treatment with removable orthodontic appliances.


The palpation phenomenon of the condyle at the external auditory meatus during treatment of Class II, division 1 anomalies by means of activators.


Soft tissue pressure in patients treated by the functional regulator.


Mechanism of apical root resorption.

Mechanism of apical root resorption.


Scanning electron microscopy of human premolars following experimental tooth movement.


Ultrastructural changes in the periodontal ligament incident to orthodontic tooth movement.


The study of the periodontium after orthodontic closure of extraction sites in the Macaca nemestrina.


Gingival status after orthodontic treatment of impacted upper canines.


The migration of teeth in adult monkeys.


Intraoral measurements on the pattern of initial orthodontic tooth movement.


A consideration of altered metabolic state in the rat and its effect on tooth movement.


Autotransplantation of the maxillary canine with the use of cell cultivation technique.


Evolutionary concepts relating language and the morphology of the oral complex.


Congenital aglossia with aplasia of several teeth and multiple impactions.


The accuracy and range of certain stereophotogrammetric measurements of facial morphology.


The estimation of dental age.

The estimation of dental age.


Changes in occlusion in the primary dentition between 2-and-one-half- and 5-and-one-half-years of age.


Changes in occlusion and craniofacial pattern during growth.


Enlarged styloid processes as a complication in mandibular prognathism surgery: report of a case.


The role of the dental assistant in teaching oral hygiene.


St. Apollonia is beginning to smile.

St. Apollonia is beginning to smile.


Involvement in international volunteer dentistry on the student and recent graduate level.


Women's place in dentistry is changing.

Women's place in dentistry is changing.


Who's the male chauvinist pig in dental school?


Dental education in Russia: a first hand look.


The effect of cancer chemotherapeutic agents on the oral mucosa.


Dental practice: 1990. Does it have to be that way?


Proliferative properties of malignant cell populations cultured in intraperitoneal diffusion chambers.


The expanding roles of operating dental auxiliaries.


Critical evaluation of scientific literature: Nine essential questions.


Statement on the expanded fuction dental auxiliary.


Perspectives in patient care. Clinical findings and history.


Fast growing non-malignant tumour of a finger.


Masticatory muscle: a preview for the rationale of centric closure position.


The 3-D Universal Bracket.

The 3-D Universal Bracket.


Assisting the eruption of impacted second bicuspids.


Modular coil spring.

Modular coil spring.


Retention.

Retention.


Orthodontics in Israel.

Orthodontics in Israel.


Consumer needs and perceptions: developing new types of dental auxiliaries.


Pit and fissure sealants.

Pit and fissure sealants.


X-ray fluorescence spectroscopy of a periapical mass of tooth.


Technique for removing fluorosis stains from vital teeth.


Blood and saliva glucose levels in relation to gingival health.


Scope and limitations in teaching fixed partial prosthodontics in India.


The relationship of the maxillary antrum and the roots of the teeth. A radiological study.


Cleft palate prosthesis.

Cleft palate prosthesis.


Treatment of grooves with sealants utilizing acid etching.


APF systems for dental caries control.

APF systems for dental caries control.


Preservation of the alveolar ridge: a simplified technique for retaining teeth beneath removable appliances.


[Sialolithiasis of the major salivary glands].


Third party dental funding in Sweden.

Third party dental funding in Sweden.


Dental material--a short guide for the general practitioner. 1. Composite restorative materials.


Third party dental funding in Germany.

Third party dental funding in Germany.


Dental education in Ireland.

Dental education in Ireland.


Oropharyngeal cancer: a review.

Oropharyngeal cancer: a review.


Occlusal adjustment by grinding.

Occlusal adjustment by grinding.


Team dentistry: the future with expanded duties auxiliaries.


Drug interactions in dentistry.

Drug interactions in dentistry.


The response to distension of the pulmonary vein-left atrial junctions in dogs with spinal section. 1. A reflex increase in heart rate in response to pulmonary vein distension was observed in decerebrate dogs. This increase could not be totally abolished by treatment with both propranolol and bretylium tosylate. Only bilateral cervical vagotomy abolished the reflex increase in heart rate.2. A significant increase in heart rate occurred in a total of seventeen spinal dogs during pulmonary vein distension.3. In seven spinal animals in which blood pressure was maintained by the continuous infusion of noradrenaline, the increase in heart rate could be totally prevented by cervical vagotomy.4. The time course of the increase in heart rate observed in the spinal animals was rapid, reaching maximum expression within 10 sec of pulmonary vein distension. Such a time course is dissimilar from that associated with pulmonary vein distension in intact or decerebrate dogs in which maximum increases in heart rate take 1-3 min to develop.5. It is concluded that the reflex tachycardia resulting from pulmonary vein distension may be mediated by both an efferent sympathetic and an efferent vagal pathway, the relative significance of each component being dependent upon the prevailing autonomic drive existent in the animal at any specific time.


Principles of the treatment of persons affected by cleft formation at the Thallwitz Clinic.


The passive eloquence.

The passive eloquence.


Passive eloquence: and a nurse's comment.


Spotlight on the clinical nurse specialist.


A contrasting psychiatric scene.

A contrasting psychiatric scene.


The clinical specialist: improving patient care.


A Norwegian nursing experience.

A Norwegian nursing experience.


Case for clinical nursing.

Case for clinical nursing.


Wound healing following tooth extraction. Histochemical study in rats.


Effect of hydrocortisone on rat tooth germ. Histochemical study.


Healing process after root canal therapy in immature human teeth.


"Accountability--the implications".

"Accountability--the implications".


Results of longterm iodine prophylaxis of endemic goiter in Yugoslavia.


Iodine prophylaxis in conditions on an European continental country.


The spectrum of iodine malnutrition and suggested management. Studies in Iraq and Iran in the Tigris river basin.


The Kangra valley experiment: prevention of Himalayan endemic goitre with iodinated salt.


Iodine nutriture and prevalence of endemic goiter in USA.


Epidemiology of endemic goitre in Piedmont (Italy).


Epidemiologic distinctions of 2 neighbouring goiter districts, of which one has had iodine prophylaxis for 10 years.


Goitrogenic action of cyanogenic glucosides present in cassava: a possible etiologic factor of endemic goiter in the Idjwi island.


Biochemical defects in thyroid hormone synthesis and their relation to endemic goiter.


Iodine deficiency, environmental goitrogens and genetic factors in the etiology of endemic goiter.


Pathophysiology of Himalayan endemic goitre.


The nodularity of nodular goitre.

The nodularity of nodular goitre.


A comparison of the clinical features in endemic cretinism.


Thyroid cancer, Quervain's thyroiditis and Hashimoto's disease in an endemic goiter Andean area.


131-I-therapy of malignant thyroid disease in regard to its classification.


Cavitating splenic infarction.

Cavitating splenic infarction.


Reality therapy: a group psychotherapeutic approach with adolescent alcoholics.


Psychodrama and alcoholism.

Psychodrama and alcoholism.


Alcoholism as viewed through family systems theory and family psychotherapy.


Group characteristics of alcoholics: a videotape demonstration.


How a therapist can use Alcoholics Anonymous.


Pastoral care in alcoholism.

Pastoral care in alcoholism.


Some recent attempts to inculcate controlled drinking in patients resistant to Alcoholics Anonymous.


Biofeedback training for anxiety tension reduction.


Decreased alcohol intake associated with the practice of meditation: a retrospective investigation.


Denial, deceptive maneuvers, and consistency in the behavior of alcoholics.


Depression in alcoholism.

Depression in alcoholism.


A jigger of alcohol, a dash of depression, and bitters: a suicidal mix.


The alcoholic's perception of self: a retrospective comparison of mood and behavior during states of sobriety and intoxication.


The role of cognitive factors in the memory disorders of alcoholic patients with the Korsakoff syndrome.


Group therapy and alcoholism.

Group therapy and alcoholism.


Hepatic phosphorylase deficiency. Its differentiation from other hepatic glycogenoses.


A comprehensive data base in general practice records.


Problem orientated personal (POP) medical records for general practice.


Report from a health centre.

Report from a health centre.


Training for health services receptionists.


It's time to discard the 8 x 5 card.

It's time to discard the 8 x 5 card.


Private hospitals in New Zealand.

Private hospitals in New Zealand.


Social work in a New Zealand group medical practice.


Alcoholism--the family disease. The sixth Leonard Ball Oration, 1973.


Divergent properties of two human lymphocytic cell lines isolated from a single specimen of peripheral blood.


Vasodilator mechanisms in the small intestine. An experimental study in the cat.


Orbital deposit in acute myeloid leukaemia.


Occult carcinoma of the breast.

Occult carcinoma of the breast.


The cellular manifestations of extrauterine cancer.


The dialysis nurse: a special breed?

The dialysis nurse: a special breed?


Clinicopathological Conference of the Medical University of South Carolina. "A young man with recurrent hemoptysis and renal failure".


"Accessory oral tonsils"--a cytologic dilemma.


Hospital physician relations.

Hospital physician relations.


Editorial: The prevention of tuberculosis.


Gastric cytology: experience in a district general hospital.


Method of calculating mechanical power in swimming the breast stroke.


Shoulder muscle activity during selected skills on the uneven parallel bars.


Rhinosporidiosis.

Rhinosporidiosis.


Preliminary exercise: a two-factor theory.


Humidification of anesthetic gases for children.


Prediction of first grade academic performance from kindergarten perceptual-motor data.


Effects of movement complexity on choice reaction and movement times.


Statistical models for inter- and intraindividual variability.


Analysis of Cratty's Locomotor Agility Test.


Duration and frequency of training as determinants of coronary tree capacity in the rat.


The mechanics of toppling techniques in diving.


Criterion score for multiple trial measures.


VanGuard Model G-and modifications for 16-mm film analysis in biomechanical research.


Apparatus to move objects in three dimensional space.


Environmental chamber for the swimming of small animals in hypoxic conditions.


A simple antrhopometric method for calculating segmental leg limb volume.


Attitude testing examining certain psychological correlates of physical activity.


Skeletal fluorosis: its importance to fluoridation and the therapy of osteoporosis.


Clinical dental photography.

Clinical dental photography.


Preparation for simple bridgework (part 3).


Coping with third-party dental programs.


Body image in orthodontics.

Body image in orthodontics.


Coping with third party dental programs.


Countering dental radiation misconceptions.


Carving--a lesson in tooth morphology.

Carving--a lesson in tooth morphology.


Three cheers for preventive dentistry but is it enough?


A formal education program fights dental disease.


Preventive dentistry: solution to unmet needs.


Preventive dentistry is catching on in England.


Try a little psychology to get prevention going.


What you and your patients need to know about preventive dentistry.


Let's sell prevention on prime time TV.

Let's sell prevention on prime time TV.


Get out your crayons, it's coloring time.


Dentistry's moon shot: caries prevention.


Are you ready for a new kind of clinic?

Are you ready for a new kind of clinic?


QC in the dental office.

QC in the dental office.


Take your pick? An operatory for every need or whim.


Voyage to the center of the condyle.

Voyage to the center of the condyle.


The placebo effect as an aid to pain control in dentistry.


A new idea in dental education.

A new idea in dental education.


The Baylor spring clinic: an experiment in continuing education.


Take a look at the hospital internship.

Take a look at the hospital internship.


Overseas clinics give new dimension to dental education.


A look at subperiosteal mandibular implant dentures.


Life is great today--what will it be like tomorrow?


Magic words won't bring success to your new practice.


How to understand the anxious patient.

How to understand the anxious patient.


What you should have on hand for office emergencies.


Should dentists charge for filling out forms?


Dental insurance will be a factor in your practice.


Is a national exam a good alternative to state boards?


What is student imput to dental education?


A place for the double degree.

A place for the double degree.


BDS, LDS: your counterpart in Britain.

BDS, LDS: your counterpart in Britain.


Troublesome trends in dentistry.

Troublesome trends in dentistry.


Educational trends through the eyes of a review course director.


No man is an island. State board's interest in educational involvement.


Dental education in Southern California versus New York: some personal observations.


Anhidrotic ectodermal dysplasia.

Anhidrotic ectodermal dysplasia.


Evaluation of drug utilization by dental patients. II. Drugs prescribed by dentists in private practice for home use.


Osteogenesis imperfecta and dentinogenesis imperfecta: report of a case.


The carousel endometer.

The carousel endometer.


Further studies on the quantitation of root canal medicaments.


Modern operating techniques applied to endodontics.


The course and incidence of multiple canals in the mesio-buccal root of the maxillary first molar.


Serial extractions.

Serial extractions.


New Jersey dental school history and progress.


Multiple canals presence and significance.


Dentistry in the Soviet Union today.

Dentistry in the Soviet Union today.


Retirement is a full time job.

Retirement is a full time job.


Dental experience in prepaid group practice.


Will my real license please stand up. II, and counting.


Root canal anatomy of the mandibular first molar.


Desensitizing effects of iontophoresis.

Desensitizing effects of iontophoresis.


A look into the crystal ball.

A look into the crystal ball.


Internal resorption.

Internal resorption.


CHIP: the administration's comprehensive health insurance plan.


Unusual foreign body.

Unusual foreign body.


Epithelial origin of some jaw neoplasms.


Thrombocytopenia. Secondary to antituberculous therapy.


Endodontics A to Z.

Endodontics A to Z.


Strain on the hard tissue of human extracted teeth due to external force.


Dynamic changes in jaw bones of rabbits and dogs during occlusion, mastication and swallowing.


Ankylosis and partial anodontia in twins.


Mechanism of dynamic responses of the canine and human skull due to occlusal, masticatory and orthodontic forces.


Antecedent-consequent variables of N2O-O2 administration in state 1 analgesia. V. The anxiety-relaxation profiles.


The influence of today's issues on the role of the dental auxiliaries.


The silver amalgam restoration.

The silver amalgam restoration.


The role of radiological interpretation in disease of the temporomandibular joint.


Remodelling of the temporomandibular joints.


Surgery of the temporomandibular joint.

Surgery of the temporomandibular joint.


Remineralization of enamel etched with strong acids.


The role of dental auxiliaries tomorrow--dental hygiene.


Dental manpower research in Minnesota.

Dental manpower research in Minnesota.


A qualitative microradiographic study of the enamel and the dentine in groung sections of impacted human permanent teeth.


The sensitivity of dentin. Changes in relation to conditions at exposed tubule apertures.


Changes in masticatory function after surgical treatment of mandibular prognathism. Cineradiographic study of bolus position.


Editorial: A comment on salivary IgA in periodontal disease.


William Hunter's "oral sepsis" and American odontology.


An historical survey of anthropometric instruments and techniques utilized in determinations of cranial dimensions and anatomic relationships in dentistry with special consideration of roentgenographic cephalometry.


Profile of a developing dental school: State University of New York at Buffalo.


Oddments in dental history: dentistry and marital bliss.


Self-instruction as a means for teaching the history of dentistry.


The senile tremor patient.

The senile tremor patient.


Temporary TMJ immobility resulting from a maxillary tuberosity injection. A case report.


Response of spontaneously discharging units to thermal stimulations.


Eruption hematoma: report of a case.

Eruption hematoma: report of a case.


On the specificity of spontaneously discharging units in the cat inferior alveolar nerve.


Lesions of the jawbones. II. Differential diagnosis of jawbone lesions.


Approaches to combination chemotherapy in rat, mouse, and hamster tumors.


The Bennett Movement and its influence in creating occlusal morphology.


Oligonucleotides of ribosomal 28 S RNA in human leukemic cells and normal lymphocytes.


Dental education and practice in the U.S.S.R. A personal view.


The use of leucocytes as an aid in the diagnosis of glycogen storage disease type II (Pompe's disease).


Coping with third-party dental programs.


Vac-ejection aspiration.

Vac-ejection aspiration.


A disease control program.

A disease control program.


The growing menace of drug interactions.


The Doctors Newmark--preview of tomorrow's practice.


Would you flunk the "POC" test?

Would you flunk the "POC" test?


Know your patients and enjoy your practice.


Incorporate? Your questions answered.

Incorporate? Your questions answered.


Why not buy a dental practice.

Why not buy a dental practice.


Phase IV: the semi-thaw.

Phase IV: the semi-thaw.


The economic ages of man: your game plan for survival.


Tips for successful hiring.

Tips for successful hiring.


The fastest cash that money can buy.

The fastest cash that money can buy.


When your investment says, I believe in me.


Getting started with a group: easy does it.


Doctors talk back about unions.

Doctors talk back about unions.


The no-knock tax audit: better know your rights.


New hope for the hopelessly uninsurable.


This office chart does double duty.

This office chart does double duty.


Making 7 per cent the easy way.

Making 7 per cent the easy way.


The best investment philosophy for you.

The best investment philosophy for you.


The influence of moisture contamination on compressive, tensile and shear stresses, volume fraction of voids and microstructure of zinc-containing and zinc-free amalgams at body temperature.


Histopathologic response of gingival tissues to hemodent and aluminum chloride solutions as tissue displacement materials.


Dental debris and calculus in children.

Dental debris and calculus in children.


Microleakage. 3. Methods of studying the adaptation of restorations to cavity walls and margins.


Terminal neuralgia, tic douloureux primary or major neuralgia, major tic neuralgia quinti major.


Precautions for patients with systemic disease seeking dental treatment.


A clinical study of the effect of changing the orientation plane on the value of sagittal condylar path inclination.


Wear of acrylic teeth.

Wear of acrylic teeth.


A problem of oral hygiene in Egypt.

A problem of oral hygiene in Egypt.


A comparative study of varying the surface texture and shape of pins on compressive and shear stresses of amalgam restorations and cores for crowns.


The interglobular spaces in the dentin in the root portion and its relation to the granular layer of tomes.


Some bioengineering properties of the intra-articular tissue.


The influence of certain abrasives on the surface texture of amalgam. An investigation and a technique.


The use of fluorescent dye for detecting the relative sealing efficiency of various root canal sealers. II. Qualitative assessment for the effect of aging.


Prognosis of root canal treated teeth among a group of Egyptians; clinical, radiographic and histologic study.


Clinical and radiographic study of postoperative healing potentiality of periapical lesions as correlated to certain histopathologic examinations.


Study of the morphological variations of the first permanent molar in the pairs of twins.


Ameloblastic adenomatoid tumor (Adenomeloblastoma).


Dental health of primary school children in Egypt: an incremental care program.


The influence of preparing filled roots for intra-radicular posts on the sealing efficiency of the remaining apical filling.


Telescope retainers in prosthodontic restorations.


The use of grafts in periodontal surgery. II. The utilization of pedicle grafts in mucogingival surgery.


Biologic studies of the root and its connective tissue environment.


Dental treatment needs in young adult Israelis.


Lichen planus.

Lichen planus.


Performance of the lingual ascorbic acid test.


A comparative study of radicular, dentigerous and primordial cysts.


The Odontological Society--its three year gestation period.


Radiographic diagnosis.

Radiographic diagnosis.


An experimental study to determine the effect of varying current intensities on surface texture and dimensional accuracy of electroformed copper dies.


Solitary neurilemmoma of the oral cavity. Report of a case.


Plasma clot welding of nerves. (Experimental report).


Sodium and the cachexia and hypophagia of tumor growth.


Heterotransplantation of human malignant tumors in "nude" thymusless mice. II. Malignant tumors induced by injection of cell cultures derived from human solid tumors.


Genesis of the Ph chromosome.

Genesis of the Ph chromosome.


Technical suggestions. Electrode carrier for percutaneous cordotomy.


Nutrition in periodontal disease.

Nutrition in periodontal disease.


Radiographic interpretation.

Radiographic interpretation.


Grievance prevention.

Grievance prevention.


Dental administration and programming.

Dental administration and programming.


Immunological aspects of prevention.

Immunological aspects of prevention.


The patient and the partial denture.

The patient and the partial denture.


Adhesion, cementation, and the dental cements.


What's your diagnosis? Dentinogenesis imperfecta.


Demonstration project phase two.

Demonstration project phase two.


Soft tissue surgery.

Soft tissue surgery.


Dental litigation.

Dental litigation.


Occult adenocarcinoma of the pancreas in a 17-year-old patient with immunosuppressed leukemia.


Folk medicine in North Carolina.

Folk medicine in North Carolina.


State mental hospital referrals: patient abandonment by local medical resources.


Experience with a skin cancer detection clinic at a state fair.


EDITORIAL: Folk medicine.

EDITORIAL: Folk medicine.


Editorial: Need our 'cries' be 'bootless'?


Editorial: The legislative vigil.

Editorial: The legislative vigil.


Editorial: On a raise in dues.

Editorial: On a raise in dues.


The Council on Education. A report.

The Council on Education. A report.


Council on Dental Care Programs. Notes on prepayment.


Reciprocity--where now?

Reciprocity--where now?


Unusual incidence of an ameloblastoma. Report of a case.


The dentist and the mentally retarded.

The dentist and the mentally retarded.


Professional responsibility.

Professional responsibility.


Peripheral giant cell reparative granuloma.


Mediation procedures. A short survey.

Mediation procedures. A short survey.


Myeloproliferative disorder with lytic osseous lesions and chromosomal anomalies.


Editorial: Dental research in Canada.

Editorial: Dental research in Canada.


Your security. Why you must consider malpractice insurance.


Search for safety.

Search for safety.


Proposal for a core auxiliary program.

Proposal for a core auxiliary program.


Dental research: avocation or vocation.

Dental research: avocation or vocation.


Current problems to a dental research career.


A clinical evaluation of nitrous oxide-oxygen sedation for dental procedures.


Single appointment therapy for a periodontal abscess.


Broad spectrum antibiotics: tetracycline, ampicillin and cephalexin.


Editorial: Federal support for graduate training.


Editorial: The manpower needs of dental education and dental research.


Identifying leadership careers in the dental profession.


Computer simulation of selected curriculum changes in dental education.


Dental education in Brazil.

Dental education in Brazil.


An interdisciplinary dental training program in a hospital.


Effective learning by slide-guide method: evaluation of a course in microanatomy.


Editorial: English: the number one language of medicine.


Folded omentum presenting as hepatosplenomegaly.


Aortography before and after surgical correction of renovascular hypertension.


Practical uses of the membrane oxygenator.


Primary adenocarcinoma of the vermiform appendix.


Ascitic fluid in tuberculosis and heart failure.


The New Jersey Foundation for Health Care Evaluation: an interim report.


Tuberculous peritonitis in association with cirrhosis of the liver.


Psoriasis and phenformin hydrochloride: a report on 2 cases.


Editorial: Evaluation mechanisms in oral surgery.


Letter: Complication conference as an education mechanism.


Letter: Reliability of CVP questioned.

Letter: Reliability of CVP questioned.


A histologic study of the regeneration of the inferior alveolar nerve.


Use of sodium morrhuate in the management of hemangiomas.


The effect of the repeated application of mouthwashes to the mucosa of the hamster cheek pouch.


Clinical-Pathological Conference. Case 6. 2. Carcinoma of the maxillary antrum in an 8-year-old child.


The effect of surgical procedure on salivary electrolytes.


Recurrent parotitis in childhood.

Recurrent parotitis in childhood.


Bacteroides infection of the mandible with secondary spread to the neck.


Mandibular resection and reconstruction for ameloblastoma in a severe diabetic.


Salivary retention phenomenon following surgical lowering of the floor of the mouth.


Cavernous sinus thrombosis. Report of a case.


Oral manifestations of Crohn's disease.

Oral manifestations of Crohn's disease.


Ameloblastoma: report of two cases.

Ameloblastoma: report of two cases.


A simplified sigmoid notch retractor for extraoral osteotomies.


Advanced educational program in oral surgery Woodruff Medical Center Emory University, Atlanta.


Refractory ulcerative stomatitis: report of a case in a patient with Hodgkin's disease.


Benign migratory glossitis associated with lichen planus.


Renal scan images with 99mTc-TPAC and 203Hg-chlormerodrin according to kidney function.


Editorial: Getting the facts straight.

Editorial: Getting the facts straight.


What dental malocclusion means.

What dental malocclusion means.


Needle biopsy of the spleen.

Needle biopsy of the spleen.


Department of Health Office of Radiation Control City of New York.


In defense of the endodontic stabilizer.


Permeability beneath orthodontic bands: variations dependent on cement type and cement-removal method.


Glycogen-storage disease associated with phosphorylase kinase deficiency: evidence for X inactivation.


The isospatial arch concept.

The isospatial arch concept.


Soft-tissue profile change associated with surgical correction of the prognathic mandible.


Surgical correction of mandibular prognathism. I. A cephalometric report.


Orthodontic aspects of musical instrument selection.


The AAO library: a developing center for membership services.


Editorial: Mutual appraisals of doctor-patient relations.


Ascorbic acid levels in phagocytic cells.


Biochemical determinations in a recently investigated case of McArdle's disease.


A general theory of ATP synthesis and utilization.


Energy coupling in mitochondria.

Energy coupling in mitochondria.


Energy transduction in the nerve membrane and studies of excitation processes with extrinsic fluorescence probes.


Properties and function of proteins in excitable membranes: an integral model of nerve excitability.


Dipole mechanisms of electrical, optical and thermal energy transductions in nerve membrane.


New ionophores for old organelles.

New ionophores for old organelles.


Energy and negentropy in enzymic catalysis.


Isolation of ionophores from mitochondria.


Addition of chemical and osmotic energies by ligand-protein interactions.


Structural aspects of energy conversion in muscle.


Elementary processes in the hydrolysis of ATP by sarcoplasmic reticulum membranes.


The chemical composition and photochemical activity of chloroplast grana and stroma lamellae.


Protein arrangement in chloroplast membranes: studies with P-diazonium-benzene-35S-sulfonic acid.


Models for energy transduction and transfer in biological systems.


Mobility and reading aids for the blind: recent developments in rehabilitation devices.


A general perspective of the quantum mechanical foundations of energy transduction.


Bilateral sudden deafness in Cogan's syndrome.


Symposium on squill. Introduction.

Symposium on squill. Introduction.


The pharmacology of squill.

The pharmacology of squill.


Monitoring the human abortus for developmental defects.


Speech sound discrimination skills of seven- and eight-year-old cleft palate males.


Effect of vitamin A on the potentiality of rat palatal processes to fuse in vivo and in vitro.


Quantitative analysis of cleft palate casts. A geometric study.


Cephalometric comparisons on the cranial base and face in individuals with isolated clefts of the palate.


Cleft palate parent groups.

Cleft palate parent groups.


Object identification and naming in cleft palate children.


Adenoidal atrophy, velopharyngeal incompetence and sucking exercises: a two year follow-up case report.


Assessment of articulation for children with cleft palate.


Noninvasive electrometric detection of nasal escape in patients with rhinophonia.


A new method for correction of cleft lip nasal deformities.


Review of diastrophic dwarfism associated with cleft palate--presentation of a case.


Cleft lip of Ayagutak, the Eskimo.

Cleft lip of Ayagutak, the Eskimo.


The ubiquitous foreign body.

The ubiquitous foreign body.


Freedom is worth fighting for.

Freedom is worth fighting for.


The effect of cleft palate on oral port constriction during fricative productions.


Alveolar soft-part sarcoma.

Alveolar soft-part sarcoma.


Anterior myocardial infarction complicated by right bundle branch block and left anterior and posterior fascicular block. A clinical survey with reference to the literature.


The drug-induced glaucomas.

The drug-induced glaucomas.


Lomotil intoxication in pediatric patients.


Necrosis of maxilla in patient with herpes zoster. Report of a case.


Stability of treatment results in class II malocclusion corrected by full mandibular advancement surgery.


Unusual sequelae to surgical repositioning of an impacted premolar.


The lingual thyroid. Abbreviated case report.


Review of a technique in the treatment of mucoceles.


Studies on oral lichen planus. II. Follow-up on 200 patients, clinical characteristics, and associated malignancy.


Darier's disease of the oral mucosa. Report of five cases.


Necrotizing sialometaplasia. Report of five additional cases.


Dyskeratosis congenita with associated periodontal disease.


Median palatine cysts. Review of the literature and report of a case.


The Chediak-Higashi syndrome. Report of a case and review of the literature.


Radiographic aids in the detection of extra root canals.


Pulpal and periapical tissue responses in conventional and monoinfected gnotobiotic rats.


Chelating agents: their softening effect on root canal dentin.


An impacted deciduous first molar. Report of a case.


A study of the radiographic features of the jaws in sickle-cell anemia.


"Phantom" foreign body of lip.

"Phantom" foreign body of lip.


Unusual impacted maxillary tooth.

Unusual impacted maxillary tooth.


Supernumerary tooth.

Supernumerary tooth.


The need for dentists in the United States.


The national board examination and academic achievement.


Can quality dental education survive?

Can quality dental education survive?


The dental examiner and our changing society.


Electrical injuries: a clinical experience with 100 cases.


Studies on the methods of percutaneous renal biopsy.


Prevalence of rheumatic heart disease among Taipei school children.


A study on the quality of well water in the coastal area of Taipei County, Taiwan.


Pseudotumours of the clavicle following neck dissection.


Editorial: Dear readers, dear authors.

Editorial: Dear readers, dear authors.


On the nature of mucoepidermoid tumours of major and minor salivary glands.


Statistical evaluation of tumours of the head and neck in infancy and childhood.


Mortality following operations of the head and neck.


Primary closure of the cleft palate using a palatal mucosal flap: an attempt to prevent growth impairment.


The influence of unilateral operations, with reference to the soft tissue only, on the growth of the skull: experimental studies on rats.


Results of animal experiments on the transplantability of cialite-preserved human dura.


A technique for reconstruction of blow-out fractures of the medial orbital wall (case report).


Where are the physicians in South Carolina?


Bulletin from the S. C. Department of Health and Environmental Control.


Continuing medical education: self study and self assessment.


Treatment of facial pain by percutaneous injection of the Gasserian ganglion.


Editorial: A new era for third parties.

Editorial: A new era for third parties.


Editorial: The dental school contradiction.


Rationale for a dues raise.

Rationale for a dues raise.


Dedication address--School of Dental Medicine State University of New York at Stony Brook--March 10, 1974.


A concept of the duties and qualifications of a member of a State Board for Dentistry.


Opposing licensure of laboratories and laboratory technicians.


Interceptive periodontics.

Interceptive periodontics.


Involving the dental patient, the new team member, in health awareness.


Periapical repair: apparent or real?

Periapical repair: apparent or real?


Carcinoma of the lip secondary to solar cheilosis. Report of a case.


Letter: Dental needs vs. dental care.

Letter: Dental needs vs. dental care.


Sexual dysfunction vaginal exercises and clitoral adhesions.


Clinicopathological conference: 12-month-old child with a palpable abdominal mass.


LDH isoenzymes in some haemopoietic diseases.


Editorial: Seek and ye shall find--in the index.


Fourteen relapses of acute lymphoblastic leukaemia in a child.


Council on Dental Care Programs. Radiograph use--an interpretation of guidelines.


Letter: The DDS-DMD issue.

Letter: The DDS-DMD issue.


Letter: Importance of fluoride.

Letter: Importance of fluoride.


Letter: On high blood pressure.

Letter: On high blood pressure.


Letter: Dentistry and the ADA.

Letter: Dentistry and the ADA.


Letter: Consumer guidance needed.

Letter: Consumer guidance needed.


Letter: The councils and implants.

Letter: The councils and implants.


Project ACORDE--a new laboratory curriculum.


Books for the dentist.

Books for the dentist.


A mechanical evaluation of buccal segment reaction to edgewise torque.


The effect of orthodontic treatment on the periodontium.


Partial resection of the cartilaginous nasal septum in rats; its influence on growth.


Some aspects of lower third molar eruption.


The effect of the passive lingual archwire on the lower denture.


Overbites and overjets.

Overbites and overjets.


Principles of orthodontic office design.


Case report.

Case report.


A cephalometric evaluation of incisor positioning with the Begg appliance.


Accreditation requirements for continuing medical education in New Jersey.


Communicable diseases in New Jersey.

Communicable diseases in New Jersey.


Therapeutic drug information center.

Therapeutic drug information center.


Pseudo venous hum.

Pseudo venous hum.


Chondromalacia patella.

Chondromalacia patella.


Periodontal scleral grafts and combined grafts of sclera and bone: two-year appraisal.


Management of gingival recession and a surgical dehiscence with a soft tissue autograft: 4 year observation.


Clinical course of a periodontal-like defect without sulcular communication.


The use of laser beams for measuring tooth mobility and tooth movement: an in vitro study.


Ultrastructural studies of human gingiva. 3. Changes of the basal lamina in chronic periodontitis.


Gingival tissue oxygenation: the effect of daily application of four commercial preparations.


Letter: Pseudosarcomatous fasciitis: an unusual cause of soft-tissue calcification.


In vitro plaque prevention with fluorides: comparison of teeth with nichrome wires.


Vertical anterior relapse.

Vertical anterior relapse.


Overbite depth indicator with particular reference to anterior open-bite.


Limitations in orthodontics.

Limitations in orthodontics.


The effect of gingival fiber surgery on the retention of rotated teeth.


Cleidocranial dysostosis. Report of a case.


Development of pulmonary aspergillomas coinciding with induction of remission in acute myeloid leukaemia.


Correlation of phytohaemagglutinin-induced lymphocyte transformation with clinical manifestations of sarcoidosis.


Studies on Stachybotrys alternans. II. Occurrence, morphology and toxigenicity.


Microbiological assay of mixtures of ampicillin and cloxacillin in plasma after separation by high voltage electrophoresis.


Search for thymidine phosphorylase, nucleoside deoxyribosyltransferase and thymidine kinase in Moraxella, Acinetobacter, and allied bacteria.


Studies on Stachybotrys alternans. 3. Chromatographic separation and tissue culture toxicity test of Stachybotrys toxins.


Population, resources, and pollution, and their impact on the Hudson estuary.


Problems on pollution and water resources in the New York City metropolitan area.


N5-methyltetrahydrofolate: homocysteine methyltransferase activity in extracts from normal, malignant and embryonic tissue culture cells.


Letter: Fetal haemoglobin levels in acute myeloid leukaemia.


Specific inhibition of sarcoma-specific cellular immunity by sera from patients with growing sarcomas.


Accumulation of S-phase cells in the bone marrow of patients with acute leukemia by cytosine arabinoside.


Remission of acute myeloid leukemia: studies of in vitro colony formation by bone marrow cells. Human bone marrow contains cells which form leukocyte colonies in semisolid culture media. Each leukocyte colony arises from a single colony-forming cell which is thought to be a unipotential stem cell, and which is subject to regulation in vitro by colony-stimulating factor. In acute myelogenous leukemia variable abnormalities in colony formation by marrow cells occur. Usually colony formation either fails to occur or the colonies that are formed are small and contain fewer than 50 cells. Similar abnormalities have been described in bone marrow dysfunction preceding overt leukemia. Usually remission of leukemia is accompanied by improved cloning by marrow cells. In this study three patients are reported in whom remission was associated with impaired cloning, and one of these patients has remained in continuous remission for a further 18 months. These observations suggest that remission status is not necessarily associated with repopulation of the bone marrow by normal hematopoietic cells.


Males with chronic myeloid leukemia and the 45, XO, Ph1 chromosome pattern. Two cases of chronic myeloid leukemia in which the cytogenetic clone was 45, XO, Ph(1) are described and compared with 20 cases recorded in the literature. The 45, XO line is peculiar to the leukemic cells and is not a manifestation of constitutional mosaicism. It probably arises from a 46, XY, Ph(1) line by loss of the Y chromosome. Because of the few cases reported, one cannot be certain that these men have a better prognosis, although the median survival time suggests that this is so. Infertility is not part of this disorder.


Ventromedial hypothalamic lesions and brain catecholamines.


Cytogenetic analysis of human acute and chronic myeloid leukemic cells cloned in agar culture.


Microbial hydroxylation of acronycine.

Microbial hydroxylation of acronycine.


Gilles de la Tourette's syndrome.

Gilles de la Tourette's syndrome.


Human leukemic cells: biological action of exogenous human leukemic DNA.


Hillenbrand H:Editorial: New instrumentality: American fund for dental health.


Student peer evaluation: an adjunct to preclinical laboratory evaluation.


Behavioral sciences in dental education: broad purposes and educational objectives.


Service provided by dental hygienists.

Service provided by dental hygienists.


Factors essential to evaluation of clinical performance.


Compound, comminuted, complex maxillofacial fractures.


Methohexital induction dosage based on lean body mass.


Clinical-Pathological Conference: case 7. I.


Mesenchymal chondrosarcoma: report of case.


Restricted opening of the mouth from symmetrical bilateral hyperplasia of the coronoid processes.


Adenomatoid odontogenic tumor (adenoameloblastoma): report of case.


A case of mixed anaerobic infection of the jaw.


Treatment of facial asymmetry with a Silastic implant.


Ludwig's angina: report of case.

Ludwig's angina: report of case.


Rationale for use of intravenous glucose.


Correction of a discontinuity defect of the maxilla by bone grafting.


Modified artificial occlusal surfaces.

Modified artificial occlusal surfaces.


Temporomandibular joint function and its effect on centric relation.


Editorial: Malpractice.

Editorial: Malpractice.


Scanning electron microscopy of human female genital tract.


Defective rRNA synthesis in human leukaemic blast cells?


Improved renal biopsy technique in children.


Unusual intracranial complications of head trauma.


Alcoholism, hypercholesterolemia, and reticuloendothelial histiocytic lipidosis. Postmortem study.


Intrauterine torsion of the spermatic cord.


Cystadenocarcinoma of pancreas with staged pancreatoduodenectomy.


Maldistribution of physicians. National problem studied locally.


Snowblower injuries.

Snowblower injuries.


Psychiatric administration. Reason and experience.


An outbreak of botulism in wild waterfowl in New Zealand.


Cysticercus ovis survey: summary of three years' results.


Liver fluke, Fasciola hepatica in New Zealand. 1. A spreading parasite in sheep and cattle.


The establishment and maintenance of indwelling venous catheters in adult female pigs.


A condition resembling Chastek paralysis in cats.


Letter: Ollulanus tricuspis and Capillaria putorii in New Zealand cats.


Letter: Strongyloides westeri and Corynebacterium equi in foals.


Effect of salivary proteins on calcification of oral bacteria.


An in vitro model of an oral keratinizing squamous epithelium.


Cytochemical demonstration of adenyl cyclase in rat incisor enamel organ.


Therapy of acute leukemia.

Therapy of acute leukemia.


Synthesis, chemistry, and biological activity of 5-thiocyanatopyrimidine nucleosides as potential masked thiols.


Molecular orbital studies on the conformation of hallucinogenic indolealkylamines and related compounds. The isolated molecules and the solvent effect.


Purification and characterization of the hormone initiating sexual morphogenesis in Volvox carteri f. nagariensis Iyengar. In Volvox carteri f. nagariensis male spheroids secrete into the medium a sexual hormone which controls the initiation of the developmental pathway leading to the formation of sexual embryos. In the absence of the hormone asexual embryos are formed. Analysis of the highly purified hormone indicates that it is a glycoprotein of 27,500-30,000 daltons, composed of one peptide chain, which has a typical composition except for a relatively low content of tryptophan. The glycosidic moiety, which accounts for about 45% of the weight of the molecule, consists of pentoses, hexoses, and amino hexoses. In the bioassay the highly purified hormone stimulates 100% formation of sexual embryos at a concentration of 10(-10) g/liter and 14.4% sexual embryos at 10(-11) g/liter (3 x 10(-16) M). The activity is remarkably thermostable and resistant to different denaturing procedures.


Role of 1,25-dihydroxyvitamin D3 in maintaining serum phosphorus and curing rickets. The intravenous injection of a single dose of 650 pmoles of 1,25-dihydroxyvitamin D(3) to rats fed a vitamin D-deficient, low-phosphorus diet caused an elevation of serum phosphorus within 5 hours which reached a maximum in about 10-12 hours. This elevated serum phosphorus returned to deficiency levels 2-3 days later. On the other hand, a single injection of 650 pmoles of 25-hydroxyvitamin D(3) produced a significant rise at 12 hours, reached a maximum in 24-36 hours, and was maintained for at least 7 days. The single dose of 1,25-dihydroxyvitamin D(3) supported little calcification of bone, whereas the 25-hydroxyvitamin D(3) produced marked calcification. Six-hundred and fifty pmoles of 24,25-dihydroxyvitamin D(3) increased serum phosphorus only slightly and induced no calcification. When 1,25-dihydroxyvitamin D(3) was given each day, a sustained increase in serum phosphorus and marked bone calcification resulted. In contrast to the serum phosphorus responses, intestinal calcium transport remained high 5 days after administration of a single dose of 1,25-dihydroxyvitamin D(3). Serum calcium was not changed appreciably by any of the metabolites. Thyroparathyroidectomized rats or rats fed a diet extremely deficient in phosphate still exhibited a marked elevation of serum phosphorus in response to 1,25-dihydroxyvitamin D(3). The effect of 1,25-dihydroxyvitamin D(3) on serum phosphorus was greatly reduced in nephrectomized rats, suggesting that the serum phosphorus response to 1,25-dihydroxyvitamin D(3) may arise from an enhancement of phosphate reabsorption in the renal tubules. It is suggested that 1,25-dihydroxyvitamin D(3) cures rickets in rats by increasing the concentration of serum phosphorus rather than by increasing serum calcium concentration and calcium absorption.


Mechanism of lysergic acid diethylamide interference with rabbit antibody biosynthesis. Lymphoid cells from hyperimmune rabbits producing antibodies to a hapten, incubated in the presence of d-lysergic acid diethylamide, continued to synthesize protein at a normal rate. Isoelectric focusing analysis of the low-molecular-weight protein secreted by the cells incubated with lysergic acid diethylamide indicated two components, with pI's of 4.9 and 5.2. Immune cells not exposed to lysergic acid diethylamide secreted only 7S IgG molecules with an average pI of approximately 7.0.


Resonance Raman spectra of cobalt-substituted hemoglobin: cooperativity and displacement of the cobalt atom upon oxygenation. The resonance Raman spectra of oxy and deoxy cobalt-substituted hemoglobin (CoHb) are reported. Comparison of these spectra to those of hemoglobin, methemoglobin, cytochrome c, and model cobalt porphyrin complexes suggests that the displacement of the cobalt atom upon oxygenation of CoHb is no greater than the out-of-plane distance in five-coordinate Co(II) porphyrins, 0.15 A. Combining this distance with the expected contraction of the cobalt-histidine bond, Ibers has estimated a maximum displacement of 0.37 A for the proximal histidine with respect to the heme plane upon oxygenation, about one-third the corresponding distance estimated for iron hemoglobin. The free energy of cooperativity for cobalt hemoglobin is also estimated to be one-third that of iron hemoglobin. These results are therefore consistent with Hopfield's distributed energy model, which predicts proportionality between proximal histidine displacement and the free energy of cooperativity. By implication they support Perutz's trigger mechanism for cooperativity.


Isolation of a clone of Chinese hamster ovary cells deficient in plant lectin-binding sites. Ricin, a galactose-binding lectin with potent cytotoxic activity, was used to select a clone of Chinese hamster ovary cells with altered plant lectin-binding properties. The clone (15B) is 80-fold less sensitive to the toxic action of ricin than the parent line. In the absence of ricin, it grows both in monolayer and suspension culture with a normal generation time. Plating efficiency, however, is significantly reduced. Relative to the parent cells, its binding of the Ricinus communis lectins, Phaseolus vulgaris erythroagglutinating phytohemagglutinin, Abrus precatorius phytohemagglutinin, and soybean phytohemagglutinin is less than 7%, while binding of lentil phytohemagglutinin, wheat-germ agglutinin, and mushroom phytohemagglutinin is 17%, 40%, and 109%, respectively. In contrast, its concanavalin A binding is increased by 70%. Consistent with these alterations, crude membrane preparations of the 15B cells were found to contain the same sugars as the parent-cell membranes but in different proportions. The 15B membranes have 28% less sialic acid, 38% less N-acetylglucosamine, 49% less galactose, the same amount of N-acetylgalactosamine, and 53% more mannose than the membranes of the parent cells.


Virus-specific messenger RNA on free and membrane-bound polyribosomes from cells infected with Rauscher leukemia virus. Cells infected by Rauscher leukemia virus synthesize virus-specific RNA which can be detected by hybridization to the single-stranded DNA copy of the viral RNA. Evidence is provided that virus-specific RNA is present in free and membrane-bound polyribosomes of these cells. The relative content of virus-specific RNA, as measured by hybridization, is 6-10 times less on free polyribosomes than on membrane-bound polyribosomes. The messenger RNA associated with both classes of polyribosomes was characterized by density gradient centrifugation. In addition to a major RNA species identified as 36S RNA, at least 2 minor components in the 14S and 21S region have also been found. There is a striking difference in the distribution of these RNA species between free and membrane-bound polyribosomes.


Structure and development of neuronal connections in isogenic organisms: transient gap junctions between growing optic axons and lamina neuroblasts. We previously showed that the growth of each bundle of eight optic fibers from one ommatidium into the optic lamina of Daphnia occurs in such a way that one of the eight fibers precedes the others into the lamina. The growth cone of this lead fiber makes surface contact with undifferentiated neuroblasts near the midplane. This is followed by a glial-like wrapping of each neuroblast around the fiber. In this report, gap junctions are shown to form for a short period of time between the growing lead fiber and the neuroblast that is wrapping around it. It is proposed that these junctions may represent a morphological correlate of informational exchange between axon and neuroblast. This signaling would then reflect the fact that the sequence of axon proliferation by the lamina neuroblasts within an optic cartrdige, ultimately composed of five lamina neurons and eight optic fibers, parallels the order in which the neuroblasts undergo the wrapping reaction with the lead fiber.


Polyadenylylation and transcription following fertilization. Polyadenylylated RNA from sea urchin embryos concomitantly labeled with [(3)H]adenosine and [(14)C]uridine between fertilization and the four-cell stage was used to determine whether the RNA primers prerequisite to the massive polyadenylylation known to occur after fertilization are synthesized during oogenesis or subsequent to fertilization. Characterization of this RNA and unlabeled RNA via retention on nitrocellulose membranes and poly(U)-impregnated filters, molecular hybridization with [(3)H]poly(U), RNase resistance, oligo(dT)-cellulose chromatography, and size-distribution studies indicates that the poly(A) tracts synthesized after fertilization are predominantly appended to preexisting cytoplasmic primers of oogenic origin. Hence, if polyadenylylation is involved in the selective editing of presumptive genetic messages, this process is not confined to the nucleus unless a given codogenic transcript can undergo more than one cycle of adenylylation.


Interspersion of repetitive and single-copy sequences in nuclear ribonucleic acid of high molecular weight. Nuclear RNA of high molecular weight, approximately 15 to 30 x 10(3) nucleotides long, isolated from rat ascites cells, has been shown previously to contain both middle repetitive and single-copy sequences. However, it was not known whether these sequences were located on separate molecules or whether they were interspersed within the same molecule. The present communication distinguishes between these possibilities and shows that a large proportion of nuclear RNA of high molecular weight consists of interspersed middle repetitive and single-copy sequences.


Electron transport to clostridial rubredoxin: kinetics of the reduction by hexaammineruthenium(II), vanadous and chromous ions. The rate constants (25 degrees , M(-1) sec(-1)), activation enthalpies (kcal/mol), and activation entropies (e.u.) for the second-order reduction of oxidized clostridial rubredoxin by Ru(NH(3))(6) (2+), V(H(2)O)(6) (2+), and Cr(H(2)O)(6) (2+) at I = 0.10 have been determined or estimated to be 9.5 x 10(4), approximately 1.4, approximately -31 (pH 6.3-7.0); 1.6 x 10(4), 0.1, -40 (pH 3.5-4.5); and 1.2 x 10(3), approximately 0, approximately -44 (pH 3.5-4.0), respectively. Ionic strength dependencies for the vanadium reaction are suggestive of a direct interaction of the reductants with the Fe(SR)(4) (-1) site of oxidized rubredoxin. The results are consistent with outer-sphere mechanisms for all three reductants and an especially high inherent outer-sphere reactivity of rubredoxin. This high reactivity level is attributed to the low activation enthalpy demands of the iron-sulfur site of rubredoxin. Thus, the possibility is raised that the rate of reaction of rubredoxin with its physiological counterparts may be determined largely by the activation entropy demands imposed by the physiological reactant. Evidence is presented in support of an absolute entropy decrease of about 7.5 e.u. on going from oxidized to reduced rubredoxin, which is presumably attributable to the charge increase from Fe(SR)(4) (1-) to Fe(SR)(4) (2-) at the redox site.


Synthesis of DNA in human lymphocytes: possible control mechanism. Partially purified, isolated nuclei from lymphocytes either stimulated or not stimulated by phytohemagglutinin can equally well synthesize DNA when [(3)H]dTTP is used as precursor. Studies of DNA polymerase activity in nuclei and cytoplasm from these cells showed that the enzyme can be detected in either stimulated or nonstimulated lymphocytes. In nonstimulated lymphocytes the uptake of thymidine is very low. The use of inhibitors such as cycloheximide, arbinosylcytosine, and actinomycin D showed that a parallel existed between thymidine uptake and DNA synthesis. All the conditions in which DNA synthesis was inhibited resulted also in an inhibition of thymidine uptake.


Amino-acid sequence of the variable region of the heavy (alpha) chain of a mouse myeloma protein with anti-hapten activity. Cyanogen bromide cleavage of the heavy (alpha) chain of protein 315 (an immunoglobulin A mouse myeloma protein with anti-dinitrophenyl activity) yielded five fragments of which one (CN2), with 156 residues, contained the chain's entire variable region. Determination of the amino-acid sequence of CN2 showed that: (1) the variable region has appreciable homology (about 33% identities) with the variable region of the light chain from the same molecule; and (2) the constant-region sequence immediately following the probable transition from variable to constant domains is the same in the protein-315 alpha as in human gamma1 and mu chains (-Val-Ser-Ser-). The sequence of the cyanogen bromide octapeptide (CN5) from the carboxy terminus of the protein-315 heavy chain closely resembles the corresponding segments of human alpha and mu chains.


The effect of replacement of methionine by homocystine on survival of malignant and normal adult mammalian cells in culture. In tissue cultures of normal adult and malignant mammalian cells, homocystine has been substituted for methionine in a medium rich in folic acid and cyanocobalamin. Normal adult cells thrive. Three highly malignant cell types from three different species, including man, die.


Analysis of interphase chromosome damage by means of premature chromosome condensation after X- and ultraviolet-irradiation. Sendai virus-mediated fusion between mitotic and interphase mammalian cells causes the rapid condensation of the interphase chromosomes into distinct structures, a process termed premature chromosome condensation. This phenomenon has been used to assess the immediate action of x-rays and ultraviolet light on the chromosomes of HeLa cells irradiated in the G1 phase of the life cycle. X-irradiation produces fragmented chromosomes; but even the most finely chopped fragments retain the condensed morphology characteristic of the premature chromosome condensation of unirradiated G1 cells. For doses up to about 1800 rads, the increase in the number of fragments is linearly related to the dose. One mean lethal dose (about 100 rads) yields a net increase of 10-15 fragments per G1 cell, which is considerably larger than previous estimates based on scoring of mitotic chromosomes. Incubation of irradiated cells produces a rapid (within 2 hr) reduction in the number of fragments, indicative of a rejoining process. The decrease in the number of pieces is not accompanied by unscheduled DNA synthesis detectable by radioautography. G1 chromosomes of cells irradiated with UV light in G1 phase are not appreciably fragmented but are elongated and attenuated so that they resemble the premature-chromosome-condensation chromosomes of unirradiated S-phase cells. Both the degree of "S-like" character attained by the G1 chromosomes in a cell and the percentage of the cell population displaying the G1 --> S transition increase with the dose and incubation time after irradiation. Thus, in contrast to the immediate manifestation of damage from x-rays, the maximum induction of the "S-like" state does not occur until about 2 hr after irradiation. The "S-like" chromosomes are capable of unscheduled DNA synthesis. We suggest that the difference in chromosome morphology found after UV- and x-irradiation underlies the reason why the former, but not the latter, induces unscheduled DNA synthesis in G1 cells.


An association between globin messenger RNA and 60S RNA derived from Friend leukemia virus. The association between certain cellular RNAs and purified RNA tumor viruses prompted us to examine the possibility that specific host messenger RNAs might also be incorporated into RNA tumor viruses. Using a mouse cell line infected with Friend leukemia virus, T-3-Cl-2, which can be induced to accumulate mouse-globin messenger RNA, we show that mouse-globin messenger RNA sequences are present in viral particles purified from the culture medium of globin-producing cells. These globin messenger RNA sequences are absent from viral particles derived from T-3-Cl-2 cells that are not producing globin messenger RNA. Virus-associated globin messenger RNA sequences sediment in association with the 60S viral RNA complex as well as in free, 9S form. However, under mild denaturing conditions which result in the conversion of viral 60S RNA to 30S and smaller forms, all the globin sequences sediment as 9S RNA. Appropriate control experiments indicate that the virus-associated globin messenger RNA is resistant to degradation by exogenous ribonuclease; that exogenously added globin messenger RNA does not become associated with the 60S viral RNA complex; and that globin messenger RNA can be detected in virions derived from cells both induced for and constitutively synthesizing globin messenger RNA.


Microtubules from mammalian brain: some properties of their depolymerization products and a proposed mechanism of assembly and disassembly. Depolymerization products of purified microtubules from porcine brain were examined by sedimentation analysis and electron microscopy. The complete depolymerization mixture exhibited 36S and 6S components in concentration-dependent equilibrium, whether depolymerization was caused by low temperature or by calcium ion. These components were recognized by electron microscopy as spirals and rings, and small particles. Agarose column chromatography yielded two major fractions, a leading one comprising mostly 36S and some 6S material and a trailing one of solely 6S material. The latter had high specific colchicine-binding activity and no tendency to polymerize. For the leading peak material these properties were the converse. It is proposed that tubulin molecules (of mass 110,000 daltons) exist in two states, here called X and Y, with those of the X-state equivalent to the material found predominantly in the trailing fraction, and those of the Y-state equated with the material in the leading fraction. Participation of tubulin molecules in microtubule assembly and disassembly is discussed, based on the assumption that those of both states have longitudinal and lateral binding domains whose strengths differentially depend upon temperature and calcium-ion concentration.