Cell attachment and penetration by influenza virus. Attachment and penetration of influenza virus into clone 1-5C-4 cells were quantitatively determined by the immunofluorescent cell-counting assay. Aided by centrifugal force, more than 95% of virus inocula of five representative influenza virus strains (A(0)/PR8, A(1)/Ann Arbor, A(2)/Japan, B/Lee, B/Great Lakes) were attached to cells at a linear rate within 10 min, in contrast to approximately 35% after stationary incubation at 35 C for 2 h. By the former procedure, a proportionality between the number of infected cells and volume of inoculum was revealed which was not evident when stationary incubation was employed. Maximal binding of virus to cells occurred at 0.2 M NaCl. The salt requirement, added to evidence of pH dependence and temperature independence, indicated that the initial virus-cell union involved electrostatic forces. Virus penetration into cells, measured by the insensitivity of virus-cell complexes to antiviral serum, was linear and complete within 15 min at 35 C for all five virus strains tested. Maximal virus penetration occurred at 0.1 to 0.2 M NaCl; the process was pH- and temperature-dependent. Both virus attachment and penetration processes were partially inhibited in the presence of diethylaminoethyl-dextran.


Rapid batch method for the preparation of fluorescein isothiocyanate-conjugated antibody. A rapid, reproducible, and relatively simple method for the preparation of large quantities of fluorescein isothiocyanate-conjugated antibody is presented. Tagged globulins with fluorochrome-protein ratios of 1 and 2 were eluted from diethylaminoethyl-cellulose in batch form by using sodium phosphate-buffered 0.1 M and 0.2 M saline, respectively. The intensity and specificity of the fluorescence due to this antibody was quite high, as determined by the ability of untagged antibody to block the immunological reactivity of the fluorescent antibody and by the lack of fluorescence of antigenically unrelated material when treated under identical conditions.


Common enterobacterial antigen. II. Effect of immunization on challenge with heterologous bacilli. Studies were carried out evaluating the protective activity of immunization with common enterobacterial antigen (CA) against challenge with heterologous gram-negative bacilli. Active immunization of mice with Escherichia coli 0:14 elicited titers of antibody to CA of 1:640 or greater but completely failed to enhance resistance to challenge by mouse virulent strains of Klebsiella pneumoniae or E. coli. Similarly, two lots, 324 and 422, of rabbit antisera to CA failed to afford passive protection to mice challenged with K. pneumoniae or E. coli. A third lot, 166, of rabbit antisera to CA did passively protect mice. The protective activity of antisera 166 was demonstrated to reflect its content of antibody to another cross-reactive antigen, Re determinant, of gram-negative bacilli which has previously been shown to protect against infections with heterologous bacilli rather than any protective effect of antibody to CA. These studies failed to demonstrate any protective activity of antibody to CA against challenge with heterologous CA containing gram-negative bacilli.


Iron-binding catechols and virulence in Escherichia coli. A comparison of viral-induced unresponsiveness of phytohemagglutinin (PHA)-induced deoxyribonucleic acid (DNA) synthesis of mouse lymphocytes was made by culturing the cells under identical conditions in the presence of HEPES buffer in a humid-air atmosphere. The degree of PHA-induced DNA synthesis was found to vary, depending upon the type of ribonucleic acid or DNA virus treatment. Myxovirus, paramyxoviruses, Mengo virus, leukemic viruses, herpesvirus, and vaccinia virus caused a depression in responsiveness, whereas lactic dehydrogenase virus, adenovirus, and polyoma virus induced an increase in DNA synthesis. Inhibition of DNA synthesis was significant only if the virus was added at 0 h or within the first 12 h after PHA stimulation. Time studies indicated that leukemic and nonleukemic viruses caused similar patterns in the alteration of PHA-induced DNA synthesis.


Galactose-deficient endotoxin from urinary Escherichia coli. Analysis of 111 rough strains of Escherichia coli from known urinary infections revealed for the first time that galactose-deficient rough mutants are not exclusively a laboratory phenomenon. They exist naturally with or without an epimerase deficiency and represent natural examples of the key role of galactose in the attachment of "O" side chains to the core of bacterial lipopolysaccharides.


Effect in mice of injection of viable Candida albicans and a cell-free sonic extract on circulating platelets. The ability of intravenously injected Candida albicans to cause thrombocytopenia and clotting disorders was tested. Mice injected in this manner showed a decrease in circulating platelets and a shortening of clotting time within hours after challenge. Treatment with heparin changed the death distribution pattern, but did not increase overall long-term survival. A cell-free sonic extract prepared from viable C. albicans cells was shown to cause aggregation of platelets in vitro, and injection of the cell-free extract into mice caused a decrease in circulating platelets similar to that observed when viable organisms were injected.


Immunity to Salmonella typhimurium infection: characterization of antigens in active protection by polyacrylamide gel electrophoresis. Immunity to challenge with Salmonella typhimurium (strain SR-11) was studied in NIH/NMRI Swiss mice. Viable organisms induced the best protection, but ribosomes were the best immunogen of the subcellular fractions. Antigens with lower concentrations of protein did not induce protection as well as antigens which had higher concentrations of protein. Acrylamide gel electrophoresis of the SR-11 subcellular components, fractionation, and differential staining of the gel columns indicated that the immunogen is a high-molecular-weight glycoprotein or mucopolysaccharide.


Behavioral treatment of drug addiction: a review and analysis.


Gambling, risk-taking and personality: a bibliography.


Smoking: the 16th and 17th century response.


"All roads lead to Rome": the role of the nursemaid in Freud's dreams.


Three letters from Sigmund Freud to André Breton.


The personality of Florence Nightingale.


Streptococcus-related glomerulonephritis.


Mechanisms of hypocomplementemia in glomerulonephritis.


Extraction site material utilized as an osseous autograft in periodontal lesions: case report.


Cell-mediated and humoral immune response to acute leukemia cells and soluble leukemia antigen--relationship to immunocompetence and prognosis.


Thermodynamic aspects of developmental biology.


Use of an in vitro microcytotoxicity test to assess human tumor-specific cell-mediated immunity and its serum-mediated abrogation.


The free transfer of skin flaps by microvascular anastomoses. An experimental study and a reappraisal.


Will a wider flap have a greater viable length? Are all flaps essentially island flaps?


Advantages of the bilobed flap for closure of small defects of the face.


On the antigenicity of immediately vascularized composite homografts.


The reverse dermal-flat flap: an alternative cross-leg flap. Case report.


Diverticular disorders of the colon. 1. Pathogenesis, clinical features, and diagnosis.


Multiple sclerosis.

Multiple sclerosis.


Drunkenness as an offense in early New England. A study of "Puritan" attitudes.


Results and prospects of microbiological studies in outer space.


Criteria for distinguishing biogenic and abiogenic amino acids--preliminary considerations.


The possibility of induction of kidney and spleen tissues in adult mammals using the Levander method.


Chemical evolution. Recent syntheses of bioorganic molecules.


Determination of the velocity of spermatozoa with the aid of the Perfil'ev and Gabe slit chamber and bacterial capillaries.


Bacteriophage Mu integration: on the orientation of the prophage.


Genetic mapping of prophage Mu.

Genetic mapping of prophage Mu.


Isolation of conditional defective mutants of temperate phage Mu-1 and deletion mapping of the Mu-1 prophage.


Similarity of vegetative map and prophage map of bacteriophage Mu-1.


Estimate of absolute specific infectivity of wound tumor virus purified with polyethylene glycol.


Intermediates in the maturation of bacteriophage lambda DNA.


A sedimentation analysis of DNA found in Escherichia coli infected with phage lambda mutants.


Replicator mutants of bacteriophage lambda: characterization of two subclasses.


Bacteriophage lambda DNA synthesized by mutants in gene N.


Genetic study on temperature sensitive nature of phage phi 80.


The effect of phage infection on the synthesis of lipid A by Escherichia coli.


Summary of the genetic mapping of prophage Mu.


Association of toxoplasmosis and cats.

Association of toxoplasmosis and cats.


Screening for sickle hemoglobin--a review.


The effect of megestrol acetate on sickling.


New drugs in the treatment of sickle cell anemia.


Screening rapidly acting anti-inflammatory agents in patients with rheumatoid arthritis.


Acid-base changes in paediatric anaesthesia. The effects of passive hyperventilation.


Intracellular distribution of antibody in immunocytes responding to primary challenge with horseradish peroxidase. A new improved technic was used to follow the development of, and the intracellular antibody distribution in antiperoxidase antibody-forming cells (AFC) of the mouse popliteal lymph nodes responding to primary stimulation with horse-radish peroxidase (HRP). The first AFC were found 6 to 8 days after immunization and were all plasma cells. Antibody was concentrated in the Golgi complexes and in a few cisternae of rough endoplasmic reticulum. Subsequently, an increasing proportion of the AFC were filled with antibody, and with time the numbers of full cells and the intensity with which they stained increased. Kinetic studies of the cell changes in the lymph node medulla suggest that lymphoid cells proliferate, differentiate into plasma cells, and are then recruited as AFC. Furthermore, it was concluded that the changing intracellular distribution of antibody represents the gradual filling of the AFC with specific antibody.


The pathogenesis of Aleutian disease of mink. 3. Immune complex arteritis. Mink chronically infected with Aleutian disease virus develop a severe necrotizing arteritis affecting muscular arteries. Acute, subacute and healing lesions may be found. Extracellular deposits of host immunoglobulin and complement and, after acid elution, viral antigen can be shown by immunofluorescence technics in areas of fibrinoid necrosis and between proliferating endothelial cells. No intracellular viral antigen was found, indicating that the virus probably does not replicate in vascular structures. The arteritis of Aleutian disease appears to be the result of immune complex deposits in vessel walls.


Arterial oxygenation during one-lung anaesthesia.


Ventilator circuits for infants and children.


Simpson House.

Simpson House.


Anaesthetic literature.

Anaesthetic literature.


Measurement in cancer.

Measurement in cancer. Increased understanding of neoplasia and of the effects of treatments has stimulated more attention to the measurement of these effects than heretofore. It is argued that this activity, involving controlled clinical trials, has yielded new and useful information and in some circumstances may have more to offer the needs of optimal patient care than traditional methods of making decisions.


The bacteriology of the obstructed biliary tree.


The influence of the vagus nerves on the biliary system.


What future for computers?

What future for computers?


James Parkinson, 1775-1824.

James Parkinson, 1775-1824.


F.A.E. Crew.

F.A.E. Crew.


Surgeon Captain G. B. Scott.

Surgeon Captain G. B. Scott.


Lady Isabel MacLennan.

Lady Isabel MacLennan.


J. H. Newmark.

J. H. Newmark.


Immunofluorescent studies of skin from patients with psoriasis.


The opsonin response of human subjects to common enterobacterial antigen.


The immune response of spleen explants from primed rabbits to sheep red cells (SRC). I. DNA synthesis in the development of antibody-producing cells (PEC).


Regulation of the immune response. VI. Inability of F(ab') 2 antibody to terminate established immune responses and its ability to interfere with IgG antibody-mediated immunosuppression.


Localization by immunofluorescence of secretory component and IgA in the intestinal mucosa of the young pig.


Some hormonal and genetic aspects of human breast cancer--a review.


Late complications of tracheostomy and prolonged endotracheal intubation.


Airway obstruction in infants and children.


The long-term tracheostomy.

The long-term tracheostomy.


Inconsistency of experimental amyloidosis in mice.


Antibodies reactive with neurons in SLE patients with neuropsychiatric manifestations.


The detection of autoantibody-forming cells. II. Cells in lymph nodes and central nervous system containing antibody to myelin basic protein.


The manufacture of protein microspheres by suspension polymerization.


Success story: 25th anniversary of NIDR.


Long-term oral carbenicillin therapy in complicated urinary-tract infections.


Kinetics and mechanisms of action of drugs on microorganisms. XVI. Effect of oleandomycin and its combinations with tetracycline, chloramphenicol, erythromycin, lincomycin, and 1'-demethyl-4'-depropyl-4'-(R and S)-n-pentylclindamycin on microbial generation of Escherichia coli.


Lacrimal and instilled fluid dynamics in rabbit eyes.


Improved, rapid spectrophotofluorometric method for assay of epinephrine: a comparative study.


Immunopathologic study of an autoimmune tubular and interstitial renal disease in brown Norway rats.


Studies on protein passage through arterial endothelium. I. Structural correlates of permeability in rat arterial endothelium.


Cell replication in the aortic endothelium: a new method for study of the problem.


Bronchial lymphoid tissue. II. Functional characterisitics.


The 'English syringe'.

The 'English syringe'.


Current facts of para-osteo-arthropathy (POA).


Computerized kidney evaluation.

Computerized kidney evaluation.


The adult respiratory distress syndrome. Recognition and management.


Progressive systemic sclerosis (diffuse scleroderma). A follow-up report of treatment with potaba.


Robert W. Philip (1857-1939), pioneer of the holistic approach to tuberculosis and its voluntary movement.


Common mechanism for repellents and attractants in bacterial chemotaxis. The migrational response of Salmonella typhimurium away from compounds such as phenol, indole, acetic acid, and leucine occurs because the bacteria tumble less frequently while descending gradients of repellents. This contrasts with their response of tumbling less frequently while ascending gradients of attractants. The results of competition experiments suggest that repellents, like attractants, operate through specific receptors, and the algebraic additivity experiments indicate that repellents and attractants utilize a common memory mechanism for taxis.


Improved cadaveric nephrectomy for kidney transplantation.


A medical history of the Panama Canal.

A medical history of the Panama Canal.


The role of surgery in sickle cell disease.


The fate of failed renal homografts retained after retransplantation.


Nitrilotriacetate (NTA): human metabolism and its importance in the total safety evaluation program.


Charles Marion Swab, MD.

Charles Marion Swab, MD.


Charles Dwight Townes, MD.

Charles Dwight Townes, MD.


Astigmatism and myopia in keratoconus.

Astigmatism and myopia in keratoconus.


Leighton Francis Appleman, MD.

Leighton Francis Appleman, MD.


Antibiotic prophylaxis in cataract surgery.


James Noah Greear, Jr, MD.

James Noah Greear, Jr, MD.


Corticosteroid effects on sulphate uptake in human iris.


Albert Darwin Ruedemann, MD.

Albert Darwin Ruedemann, MD.


Members.

Members.


Focal choroidopathy in experimental ocular histoplasmosis.


Antithymocyte serum and methotrexate for control of graft-versus-host disease in dogs.


Measurement of the immune response to a renal allograft. I. Evaluation of changes in lymphocyte activity in the rat.


Allogeneic bone marrow transplantation in a patient with aplastic anemia using a phenotypically HL-A-identifcal unrelated donor.


Immunological memory in radiation chimeras.


The prediction of acute rejection in human renal transplantation using the leucocyte migration test.


Protective effect of methylprednisolone against ischemic injury to the kidney.


The use of  125 I-fibrinogen in the detection of renal allograft rejection. Rat model.


Pulsatile flow and viability of isolated perfused kidneys.


Unsuccessful attempts to control hyperacute rejection of human renal homografts with F(ab') 2  and citrate organ pretreatment.


Lymphocyte activation after renal transplantation.


Effective treatment of severely damaged kidneys prior to transplantation. I. Preliminary results.


Skin graft prolongation caused by changes in grafting procedure.


Topics in physics and middle ear surgery.


The concepts of the sick role and illness behavior.


The communication of information about illness. Clinical, sociological, and methodological considerations.


Giving up as a final common pathway to changes in health.


Immunofluorescence studies on the G cells in normal and abnormal gastric mucosa.


Studying the decision to see a doctor. Review, critique, corrective.


Individual differences in somatic perception. A projective method of investigation.


The hospital environment: its impact on the patient.


Effect of triiodothyronine on some metabolic responses of obese patients.


Oxalate metabolism. I.

Oxalate metabolism. I.


Effect of secretin and pentagastrin on gastric secretion in man.


Time factors in obesity.

Time factors in obesity.


Nonfulminant fat embolism: review of concepts on its genesis and physiopathology.


Nonsteroid anti-inflammatory agents.

Nonsteroid anti-inflammatory agents. Nonsteroidal antiinflammatory agents (NSAID) are reviewed.  Aspirin, indomethacin, and salicylate have all been shown to interfere or prevent the synthesis of prostaglandins (PGs), a main tissue inflammatory substance.  However, an exception to the correlation of PG-synthetase inhibition with antiinflammatory activity has been noted with certain arylacetic acids.  Studies on the role of lysosomes in the inflammation are criticized in this review, mainly because of improper understanding of the role of lysosomes in a physiological system.  Since lysosomes are not a specific body, but an activator, attempts to isolate lysosomes to study functions are doomed to failure; i.e., a lysosome has no definition without the rest of its self-regulating system.  NSAIDs work in 2 main ways; either via antiaggregation or antiinflammation.  This may result in drug interactions between various types of NSAIDs.  For example, simultaneous administration of aspirin with indomethacine did not produce an additive effect; rather they produced no effect in the rat paw edema test dissimilar to the action of either drug separately.  However, hydrocortisone combined with another NSAID enhanced its antiinflammatory effects.  Albumin binding theories of NSAID mechanism of action state that drugs are active only when albumin-bound because it has displaced tryptophane, or urate, or some other normally bound substance.  The only way to discover an efficient antiinflammatory agent is to obviate the need for it by discovering the actual mechanism of inflammation, for when specific anti-etiologic therapy is developed, the need for treatment with NSAIDs is irrelevant.


The pharmacology of 6-hydroxydopamine.

The pharmacology of 6-hydroxydopamine.


Chemotherapy of malaria.

Chemotherapy of malaria.


Synthetic analogs of oxytocin and the vasopressins.


Windsor Cooper Cutting (1907-1972).

Windsor Cooper Cutting (1907-1972).


Drugs and lipid metabolism.

Drugs and lipid metabolism.


Chronic central nervous system toxicity of alcohol.


Corticosteroid-induced parturition in domestic animals.


Applications of integrated gas chromatography-mass spectrometry in pharmacology and toxicology.


Application of new analytical techniques to pharmacology.


The effect of temperature on the action of drugs.


Tubular sites and mechanisms of diuretic action.


Bilateral nephrectomy and renal homotransplantation for malignant nephrosclerosis.


Parathyroidectomy for hyperplasia in renal disease.


The surgical scrub--practical consideration.


A laboratory investigation of the bond strengths of some dental cements, including three zinc polycarboxylate cements.


Leg weakness of poultry--a clinical and pathological characterisation.


Fowler mcCormick.

Fowler mcCormick.


An interesting asymmetry in a mediaeval skull.


Corneal and conjunctival calcification in chronic renal failure.


Tissue typing in corneal grafting.

Tissue typing in corneal grafting.


Communication between psychiatrists.

Communication between psychiatrists.


Effect of copper-containing intrauterine contraceptive devices on human cells in culture. Intrauterine devices containing copper were introduced into cultures of adult and fetal cells. The Dalkon shield had no apparent effect on cell growth. The Gravigard device caused inhibition of growth and an increase in copper concentration in the supranatant medium. When the latter device was transferred through a series of subcultures the rate of release of copper decreased and growth was no longer inhibited.


J.R. Wilson.

J.R. Wilson.


V.E. Lloyd.

V.E. Lloyd.


Studies on L-forms of bacteria. 1. In vitro induction and propagation.


Major-General A.E. Campbell.

Major-General A.E. Campbell.


D.M. Hume.

D.M. Hume.


W.N. Watson.

W.N. Watson.


C.H.D. Bartley.

C.H.D. Bartley.


Regulations concerning lepers. Translated from Danielssen, D.C. and Boeck, W.: Traite de la Spedalskhed. Paris, Bailliere, 1848, pp. 120-22.


Porcelain and gold--problems and answers.


Lower denture weighting.

Lower denture weighting.


Orthodontics, prior to prosthetic diagnosis.


Ludwik Dzwonkowski (1892-1972).

Ludwik Dzwonkowski (1892-1972).


Insulin secretion after long-term infusion of xylitol.


Demonstration by immunofluorescence of the lipotropic hormone-LPH in bovine, ovine and procine adenohypophysis.


Corneal surgery.

Corneal surgery.


Determination of glomerular permeability in renal transplantation using polyvinylpyrrolidone.


Vitamin E and coronary heart disease.

Vitamin E and coronary heart disease.


Candida albicans and acrylic resin.

Candida albicans and acrylic resin.


Comparative chemotherapeutic activities of heptaene macrolide antifungal antibiotics in experimental candidiasis.


Case report: vestibuloplasty--skin and palatal mucosal grafts.


Use of titanium mesh and autogenous bone marrow in the repair of a nonunited mandibular fracture: report of case and review of the literature.


Cell transfer studies on the persistence of homograft sensitivity in the mouse: the periodic redistribution of sensitised cells between the circulation and lymphoid tissues.


The cellular infiltrate in skin allografts in mice.


The histopathology of peritoneal starch granulomas.


Genesis.

Genesis.


Demonstration of sites of snake-venom localisation by immunofluorescence techniques.


Some achievements of the College.

Some achievements of the College.


Endodontic implants for lower denture stabilization. Case report.


Proposals to found a Royal College of General Practitioners in the nineteenth century.


Epidemiology of motor-neuron diseases.

Epidemiology of motor-neuron diseases.


Calcium metabolism and secretion of gastric juice.


The status of Toxoplasma gondii in the etiology of senile human cataract.


Living systems. I. The nature of living systems.


Living systems. II. The organism.

Living systems. II. The organism.


Structural organization of the fibrinogen macromolecule related to the process of fibrin formation.


Evaluation of the treatment of diabetic retinopathy. A research project.


The prognosis of corneal transplantation.


Ileal resection, or disease, and the blind loop syndrome: current concepts of pathophysiology.


Nikolai Konstantinovich Kol'tsov (on the 100th anniversary of his birth).


A method of facilitating renal transplantation with the use of stockinet.


Skin dressings in the treatment of contaminated wounds.


Evaluation of two antiseptic agents in surgical preparation of hands by a new method.


Nursing care of the cardiac patient: an annotated bibliography.


Primary reticulum cell sarcoma of the breast.


Monitoring corneal cryopreservation.

Monitoring corneal cryopreservation.


The vitreous.

The vitreous.


The geneticist and spina bifida.

The geneticist and spina bifida.


A double blind study on the effects of monosodium glutamate in man.


A double blind trial for the clinical management of psychogenic headache.


Extradural corticosteroid injection in management of lumbar nerve root compression. The effect of extradural corticosteroid injection in patients with nerve root compression syndromes associated with degenerative disease of the lumbar intervertebral discs was assessed in a double-blind controlled trial on 100 consecutive inpatients assigned by random allocation to treatment and control groups. Assessment during admission and at three months revealed statistically highly significant differences in respect of relief of pain and resumption of normal occupation in favour of the group treated by extradural injection. This treatment seems to be a valuable adjunct to the management of lumbar nerve root compression syndromes associated with degenerative disc disease.


Immunoglobulin deposition in liver of patients with active chronic hepatitis and antibody against smooth muscle. Liver biopsies from 33 patients with miscellaneous liver diseases were studied by direct immunofluorescence. Three patients with active chronic hepatitis possessed a distinctive pattern of staining with anti-IgG conjugate. They were relatively young women and all three possessed the anti-smooth-muscle antibody. In addition, all three were untreated at the time of study. It is suggested that direct immunofluorescence on liver biopsies may help in the investigation of liver disease and that humoral immunity may participate in the pathogenesis of active chronic hepatitis.


Arrhythmias in the Guillain-Barré syndrome.


Treatment of primary hyperlipoproteinaemias with a tetralin derivative (Su-13'437).


Mediterranean anaemia in antiquity.

Mediterranean anaemia in antiquity.


New horizons in medical ethics. Priorities in medicine.


E. Stengel.

E. Stengel.


Margaret D. Crawford.

Margaret D. Crawford.


J. Dennis.

J. Dennis.


Forward traction in the correction of retrodisplaced maxilla. An external fixation apparatus immobilized to the skull has been used with satisfactory results in 15 cases of significant retrusion of the middle third of the face in which intermaxillary wiring and facial suspension had proved inadequate. The retrusion was a consequence of trauma or secondary to congenital malformation.This apparatus, developed by Delbet and Tessier, would be useful in difficult cases, such as for edentulous patients, and is an alternative to bone grafting.


Type V hyperlipoproteinaemia. Metabolic changes in hyperlipaemic and previously hyperlipaemic ("susceptible") male patients.


Behavioral approaches to study and treatment of psychogenic tics.


Pre- and post-rigor fixation of muscle.

Pre- and post-rigor fixation of muscle.


Clinical trial of a chemical test for bacteriuria. A chemical test for the detection of bacteriuria-Uriglox-has been compared with the results obtained on culturing urine obtained by suprapubic aspiration in 351 pregnant or puerperal women. The results of this comparison show a correlation of 96.9%, both false positive and false negative results occurring.


The production and testing of staphylococci with clumping factor activity for use in the assay of fibrinogen degradation products. A method for the large-scale production of a staphylococcal preparation for use in the assay of fibrinogen degradation products is described. The material is assayed against a fibrinogen standard and is shown to be stable over long periods and of high sensitivity. The possibility of production on an occasional basis in a routine laboratory is discussed.


A combined moist chamber and tray.

A combined moist chamber and tray.


A fine-needle aspiration biopsy service.


The histology of chronic candidal infection of the rat's tongue and its relevance to human oral leukoplakia.


Surgical treatment of ventricular preexcitation. A report of two cases.


The clinical biochemistry of transplants.


Preoperative teaching of the cardiovascular patient.


Synthesis and biological activity of some imidazo(4,5-b)pyrazines and their ribonucleosides as purine analogs.


Nitrohistidines and nitrohistamines.

Nitrohistidines and nitrohistamines.


Potential antimicrobial furans.

Potential antimicrobial furans.


DNA replication in Escherichia coli: evidence for two classes of small deoxyribonucleotide chains.


Ultraviolet reactivation of lambda phage: assay of infectivity of DNA molecules by spheroplast transfection.


Synthesis of ribosomal proteins during the yeast cell cycle.


Lorenz Böhler: in memoriam.

Lorenz Böhler: in memoriam.


Sun Yat-sen.

Sun Yat-sen.


Psychological adaptation to renal transplantation.


Prospects for research on schizophrenia. Bibliography.


Social work for profit.

Social work for profit.


Carl Henry Alström.

Carl Henry Alström.


A review of some important studies of schizophrenic thought disorders and their implications.


Cell growth and extracellular enzyme synthesis in fermentations.


Familial lymphoma including a report of familial primary upper small intestinal lymphoma.


A composite filling material. A two-year clinical assessment.


Can mycosis fungoides begin in the epidermis? A hypothesis.


Ontogeny of pemphigus and bullous pemphigoid antigens in human skin.


Skin sampling for Candida with adhesive tape.


Xeroderma pigmentosum.

Xeroderma pigmentosum.


Leukaemic antigens.

Leukaemic antigens.


Studies of the procoagulant and fibrinolytic activity of promyelocytes in acute promyelocytic leukaemia.


Antifibrinolytic treatment in haemophilia: a controlled trial of prophylaxis with tranexamic acid.


Red cell elasticity and blood flow.

Red cell elasticity and blood flow.


John Innes, B.Sc., M.B., Ch.B., D.M.R.E.


Neurology's debt to Becquerel.

Neurology's debt to Becquerel.


Computerized transverse axial tomography.


The reduction of surgical wound infections by prophylactic parenteral cephaloridine. A controlled clinical trial.


The significance of humoral antibodies in the localization of human malignant melanoma.


A case of emphysematous pyelonephritis.

A case of emphysematous pyelonephritis.


Comparison of single-layer inverting, two-layer inverting, and everting anastomoses in the rabbit colon.


A randomized prospective clinical trial of single-layer and two-layer inverting intestinal anastomoses.


Factors governing the uptake of pertechnetate by human brain tumours. A scintigraphic study.


Is aqueduct stenosis a result of hydrocephalus?


Vascular smooth muscle updated.

Vascular smooth muscle updated.


Hospitals and state governments: a conversation with Albert W. Snoke, M. D..


Further studies of thymus-bone marrow cell synergism in cutaneous manifestations of delayed hypersensitivity to methylated human serum albumin. The effect of cortisone acetate.


An in vivo primary response to 2,4,6-trinitrophenyl substituted erythrocytes.


Transfer of antigen from macrophages to lymphocytes. II. Immunological significance of the transfer of lipopolysaccharide.


A second look at chronic gastritis a new classification.


Late cutaneous allergic responses in isolated IgE-dependent reactions.


A clinical study of methylprednisolone acetate (Depo-Medrol) in the treatment of pain and inflammation associated with various foot disorders.


Repair of hammertoe fifth: with osteoplasty head of proximal phalanx and with extensor hood release and tendon recession.


The problem of asthma.

The problem of asthma.


Effects of estrogen and progesterone on pituitary response to stimulation by luteinizing hormone-releasing factor.


Stimulation of growth hormone by luteinizing hormone-releasing hormone in active acromegaly.


Citation of the Distinguished Leadership Award of The Endocrine Society for 1973 to John S.S. Browne, M.D.


Citation for the Koch Award of the Endocrine Society for 1973 to C.H. Sawyer, Ph.D. and J.W. Everett, Ph.D.


Citation for the Ernst Oppenheimer Memorial Award of The Endocrine Society for 1973 to Richard J. Wurtman, M.D.


Citation for the Eli Lilly Lecturer of The Endocrine Society for 1973 to Hector F. DeLuca, PhD.


Citation for the Ayerst Award for Distinguished Service in Endocrinology of The Endocrine Society for 1973 to Robert M. Blizzard, M.D.


Metabolism of  125 I-luteinizing hormone-releasing hormone. With the use of prepared iodine-125-monoiodo-LH-RH, the disappearance rates of LH-RH (luteinizing hormone-releasing hormone) in man and in the rat were investigated, as well as the distribution of LH-RH in the rat.  After 10 microcuries of iodine-125-LH-RH were injected as an iv bolus into 3 male volunteers, blood samples from the opposite arm were obtained through an indwelling venous catheter at 2, 5, 8, 11, 15, 20, 30, 45, and 60 minutes postinjection  and at 6 and 24 hours postinjection.  To describe the disappearance curve a 3-term exponential equation was necessary and sufficient.  The ranges of the t1/2s for the first, second, and third components were 2-4 minutes, 30-55 minutes, and starting at more than 10 hours.  The initial distribution space was 3-4.7 1 or 37-47 ml/kg body weight.  Similar procedures in the rat (45) resulted in an equation with 2 exponentials, with the ranges of the t1/2s for the first and second components being 5-10 minutes and 150-600 minutes.  In both man and rat the distribution volume approximated estimated plasma volume.  Following the injection of iodine-125-LH-RH in the rat, pituitary radioactivity increased, as expected, reaching a maximum tissue/serum ratio of 1.5 at 90 minutes.  2 iodinated oligomers of LH-RH were found to have different disappearance rates and distributions in the rat than did iodine-125-LHrh.  the disappearance rate of iodine-125-LH-RH in this study is consistent with values for other small peptide hormones.


Enhancement of maternal behavior and nest building following systemic and diencephalic administration of prolactin and progesterone in the mouse.


Parkinsonism--two types of activation.

Parkinsonism--two types of activation.


Sensitivity of the anterior pituitary gland to luteinizing hormone releasing factor (LRF) during pro-oestrus in the rat.


Right atrium-pulmonary artery allograft for correction of tricuspid atresia.


Radioactive assessment of the reduction of passenger blood cells by cardiopulmonary perfusion in situ prior to transplantation.


Metabolic changes in ischemic lungs for evaluation of graft viability.


Peristernal wiring in closure of median sternotomy. False aneurysm of the internal mammary artery.


Edward Delos Churchill (1895-1972).

Edward Delos Churchill (1895-1972).


Stuart W. Harrington (1889-1973).

Stuart W. Harrington (1889-1973).


Functional correction of transposition of the great arteries. A new approach to avoid postoperative arrhythmias.


Surgery of proximal aorta in Marfan's syndrome.


Simultaneous avulsion of the innominate and left intrathoracic subclavian arteries. A case report.


Prevention of intraventricular haemorrhage in babies receiving artificial ventilation.


The state of health in New South Wales in the 1820's.


Role of dopamine in the nervous system.

Role of dopamine in the nervous system.


L-dopa, Parkinson's disease and pupillary reaction to light.


A native ribonucleoprotein complex from Escherichia coli ribosomes. An RNA-protein complex was isolated from the 50S subunit of E. coli ribosomes after trypsin digestion. The complex contains only one protein, L24. Treatment of the complex with pancreatic ribonuclease results in digestion of most of the RNA; however, an RNA fragment of about 100 nucleotides in length is stable to nuclease digestion and remains bound to the protein. It is also possible to reconstitute a complex from 23S RNA and isolated L24; nuclease digestion of this complex produces a resistant RNA fragment of the same size as the native complex. The protein can still bind to 23S RNA after N-methylation of about 20% of its lysine residues. Thus, by use of N-methylated L24 labeled with either (14)C or (3)H, the binding stoichiometry of the reconstituted complex was established; binding of L24 to RNA once again renders the protein trypsin-resistant. This would appear to be a good system for the study of RNA-protein interactions.


An improved bacterial test system for the detection and classification of mutagens and carcinogens. We previously described a set of four strains of Salmonella typhimurium designed for detecting the various types of mutagens, and showed their utility in detecting a wide variety of carcinogens as mutagens. The lipopolysaccharide that normally coats these bacteria is a barrier to penetration of mutagens to the cell membrane. The set of tester strains has been improved by adding a mutation (rfa: deep rough) that results in a deficient lipopolysaccharide. The techniques for using these strains for detecting mutagens are presented and the tests are shown to be extremely sensitive and convenient. The specificity of frameshift mutagenesis is clarified. As adjuncts to the test with the four strains, we describe a test that compares mutagenic killing in deep rough strains with and without DNA excision repair, and a test using forward mutagenesis in a deep rough strain lacking excision repair.


Synthesis of stable RNA in stringent Escherichia coli cells in the absence of charged transfer RNA. It has been possible to demonstrate the complete absence of either charged tRNA(Glu) or charged tRNA(Val) at 42 degrees by the use of two stringent strains of E. coli, one temperature-sensitive for glutamyl-tRNA synthetase and the other temperature-sensitive for valyl-tRNA synthetase. In both strains, stable RNA synthesis ceases, and guanosine tetraphosphate accumulates upon incubation at the nonpermissive temperature. Unique among a series of antibiotics tested, only tetracycline was able to stimulate stable RNA synthesis and to cause disappearance of the guanosine nucleotide. In this regard tetracycline and the "relaxed" gene product appear to be analogous.


Isolation of the self-regulated repressor protein of the Hut operons of Salmonella typhimurium. In Salmonella typhimurium the structural genes of the enzymes responsible for histidine utilization (hut) are clustered in two adjacent operons. A single repressor regulates both operons. The repressor itself is a member of one of the hut operons and, thus, regulates its own synthesis. We have assayed the hut repressor by its ability to bind radioactive DNA to nitrocellulose filters. The binding is specific for DNA bearing the hut operons, and the binding is abolished by the inducer, urocanate. As a member of one of the hut operons, the repressor is inducible, subject to catabolite repression, and affected by a promoter mutation.


The nature of the colicin K of Escherichia coli K235. When E. coli K235 is grown in the presence of mitomycin C it elaborates part of its colicin as a protein, which has been purified by gel filtration and electrofocusing to yield a product that is serologically homogeneous. The colicin K of E. coli K235 is chemically and serologically identical with that of Proteus mirabilis. Like the colicin of the latter, the E. coli bacteriocin occurs in multiple forms that exhibit slight differences in mobility upon electrophoresis in a polyacrylamide gel.


A stopped-flow apparatus with light-scattering detection and its application to biochemical reactions. A stopped-flow apparatus utilizing light-scattering for following the progress of a reaction is described. The method is applicable to all reactions that result in a significant change of the average molecular weight. It was possible due to several modifications of a conventional stopped-flow system to obtain a sensitivity comparable to that of commercial instruments for static light-scattering measurements. Experiments on three reactions are reported: association and dissociation of mercury ligands with DNA, dissociation of the dimers of DNA-dependent RNA polymerase, and complex formation of tRNA(Ser) (yeast) with the cognate aminoacyl-tRNA synthetase. The changes in the intensities of the scattered light are calculated and compared with the measured amplitudes.


Population analysis of the deinduction kinetics of galactose long-term adaptation mutants of yeast. By use of a selective galactose agar medium containing ethidium bromide, a population analysis of the deinduction kinetics of yeast galactose long-term adaptation mutants (gal 3) has been done. It was first determined that the gal 3 mutation is specific to the yeast galactose system and that induced cultures of gal 3 strains are capable of growth on galactose agar medium containing ethidium bromide, whereas noninduced cultures are not. Population analyses of induced gal 3 strains under going deinduction in the absence of galactose demonstrate that a minimum number of five induction units per cell are required for induction of the galactose system. It is concluded that: these induction units are actively synthesized only in the presence of inducer and are diluted out through cell division; they are stable under nongrowing conditions; they are heterogeneous in nature; at most two of the five minimum units are products of the gal 2 locus; and the other units may be three of one type, one of one type and two of another, or one each of three different types.


An RNA that multiplies indefinitely with DNA-dependent RNA polymerase: selection from a random copolymer. We have selected from a mixture of random polynucleotides an RNA that is able to replicate in the presence of the DNA-dependent RNA polymerase of Escherichia coli, provided the medium contains Mn(2+) and ITP, in addition to ATP, CTP, and UTP. The RNA consists of a chain of about 125 (+/-25) nucleotides and appears to have a defined, nonrepetitive sequence.


Neuropsychological studies in the USSR. A review. I.


Edward Glover.

Edward Glover.


Philosophical antecedents to modern theories of human aggressive instinct.


The quality of the Forth estuary.

The quality of the Forth estuary.


Rabbit antilymphocyte and antithymocyte serum pools prepared to 1,5-difluoro-2,4-dinitrobenzene-treated cells.


Antilymphocytic antibodies and marrow transplantation. I. The effect of antilymphocyte serum on xenogeneic engraftment.


Hemodialysis duration and renal transplant survival.


Renal transplantation in the inbred rat. XIX. In vitro correlates of enhancement induction.


Effect of splenectomy on human renal transplants.


Lack of correlation between periodic perfusate changes and subsequent renal function.


Combination drug treatment in immunosuppression. Effect of azathioprine, cyclophosphamide, and methylprednisolone on rabbit renal allografts.


Immune serum implements either accelerated rejection or prolonged survival of murine skin grafts.


Location of some antigens in the kidney of human embryos and adults.


The role of passenger leucocytes in the rejection of renal allografts in the rat.


Immediate complement consumption and ultrastructural lesions in canine renal allografts.


Management of the keratoplasty patient.

Management of the keratoplasty patient.


Fetal and embryonic hemoglobins.

Fetal and embryonic hemoglobins.


Different methods of regeneration.

Different methods of regeneration.


Mayan physician. Dr. Thomas Gann's medical experiences in the jungles of British Honduras.


Studies on beta-cystathionase and omicron-acetylhomoserine sulfhydrylase as the enzymes of alternative methionine biosynthetic pathways in Aspergillus nidulans.


Nervous and behavioral effects of microwave radiation in humans.


Orthodontics--mechanical or biologic objectives?


The role of the condyle in the postnatal growth of the mandible.


Historical and theoretical aspects of isoelectric focusing.


Isoelectric focusing and study of ribosomal proteins and lactate dehydrogenase.


Dedication to Professor Arne Tiselius.

Dedication to Professor Arne Tiselius.


Structure determinations of FDP aldolase and the fine resolution of some glycolytic enzymes by isoelectric focusing.


Response of hypersensitive mice to the footpad injection of living homologous or heterologous mycobacteria: preliminary report. Mice sensitized by the injection of viable mycobacteria into one of the hind footpads responded to a second injection of mycobacteria (3 to 4 weeks later), introduced into the contralateral foot, with a degree of footpad swelling that was both accelerated and exaggerated beyond that observed after the first inoculation. The degree of specificity of this reaction (i.e., response to homologous versus heterologous mycobacteria) was comparable to that previously reported for dermal reactions of hypersensitive guinea pigs to tuberculin or tuberculin-like antigens from mycobacteria. In preliminary studies it was impossible to achieve this state of specific sensitization by vaccinating mice subcutaneously with water-in-oil emulsions of heat-killed mycobacteria; reasons for the failure are discussed. It is suggested that this tool could prove useful in both taxonomic and immunological investigations. Advantages and disadvantages of the mouse footpad test in relation to the dermal skin test in guinea pigs are discussed.


Micromethod system for identification of anaerobic bacteria. A micromethod multitest system prepared by Analytab Products, Inc. and conventional tests employed at the Center for Disease Control for identification of anaerobes were compared. All procedures were conducted in an anaerobic glove box. A total of 104 cultures, including 18 reference strains and 86 diagnostic cultures, were examined. Ninety-one percent of the total tests performed with the two systems were in agreement. Greater than 90% agreement between the two systems was obtained with 12 of the 17 differential tests compared. The tests for nitrate reduction and H(2)S production gave the poorest agreement, 77.8 and 80.8%, respectively. Only 66% of the 86 diagnostic cultures could be presumptively identified with the micromethod system supplemented only with microscopy and colonial characteristics. However, when appropriate supplementary tests and gas-liquid chromatography were used with the micromethod system, 85% of the 86 strains could be identified. When Ehrlich reagent, instead of Kovac reagent, was used with the micromethod to test for indole, the agreement in identification was raised to 93%.


Automation of the indirect fluorescent-antibody test for toxoplasmosis. The feasibility of automating the decision-making tasks of the medical technician in the determination of the results of the indirect fluorescent-antibody test for toxoplasmosis was investigated. Two approaches were studied: (i) macroscopic measurement of fluorescence from a large number of organisms (full-field illumination measurements), and (ii) microscopic measurements of fluorescence and morphology of individual parasites (pattern recognition). The macroscopic approach was studied utilizing an argon-ion laser in incident illumination with a Leitz Ortholux microscope and a phototube mounted so as to measure green fluorescence. Due to similar amounts of fluorescence from negative polar reactions and positive rim reactions, the macroscopic technique was concluded to be not feasible. The microscopic approach was studied utilizing a high resolution optical pattern recognition instrument. The results indicate that an object area measurement is sufficient to distinguish the presence of Toxoplasma gondii organisms from debris and overlapping organisms when studying the red fluorescence (due to Evans blue counterstain). Dark reactions were determined by the absence of green fluorescence. The differentiation of the rim and polar reactions was determined by the measurement of the ratio of green fluorescing area (due to fluorescein isothiocyanate) to red fluorescing area on the same organism. Clinically important titer information was also demonstrated to be obtainable. It was concluded that complete automation of the indirect fluorescent antibody test for toxoplasmosis is feasible with existing technology.


Isolation of salmonellae from pork carcasses. Four hundred and twenty pork carcasses from four abattoirs were examined for the presence of salmonellae by use of swabbing-enrichment techniques and contact plate methods. Carcasses from only one abattoir were found to be contaminated by swabbing-enrichment (23.3%) and contact plate (17.9%) methods. The area of the skin side of the ham, near the anal opening, was determined to be the area to examine for isolating salmonellae from pork carcasses with the greatest frequency. The most frequently isolated species of salmonellae in this study were Salmonella derby, S. anatum, S. typhimurium, and S. indiana.


Bactericidal effect of various combinations of gamma radiation and chloramine on aqueous suspensions of Escherichia coli. Methods of combining gamma radiation with chloramine to disinfect aqueous suspensions of Escherichia coli were investigated. Logarithmically grown cells were exposed to the bactericidal agents sequentially (i.e., radiation followed by chloramine, and chloramine followed by radiation) and simultaneously. Regardless of which combination was used, the bactericidal effect was always less than additive. During the phase of work involving the simultaneous addition of both agents, it was observed that chloramine was destroyed more rapidly by radiation than were the organisms. Since an increase in the bactericidal effectiveness of either disinfectant by prior or simultaneous treatment of the cells with the other disinfectant was not achieved in buffered distilled water, it was concluded that disinfection of wastewater effluents by combining ionizing radiation with chloramine would not be economically feasible.


Abolition of swarming of Proteus by p-nitrophenyl glycerin: general properties. Para-nitrophenyl glycerin (PNPG) was shown to be an effective agent to abolish the swarming of Proteus mirabilis and Proteus vulgaris on predried solid culture media. The level required to abolish swarming varied with the strain of Proteus, the components of the medium, and also with the conditions of incubation. Generally 0.1 to 0.2 mM PNPG effectively abolished swarming for at least 24 h with aerobic incubation. Levels of PNPG that abolished swarming showed no effect upon the growth of the cells, little or no effect upon the motility characteristics of the organisms, and no effect upon the cellular morphology. PNPG was found to be freely water soluble, stable to autoclaving, and to retain biological activity for at least one month in prepared culture media stored under refrigeration.


Resistance to arbovirus challenge in mice immediately after vaccination. Mice vaccinated with a single injection of formalin-inactivated suspensions of mouse brain tissue infected with arboviruses were markedly protected against a challenge injection administered hours later. The protection observed during the first 2 days after vaccination seemed to be nonspecific, in that it appeared not only with the homologous system but also between arboviruses of different antigenic groups; this phase may be associated with an interferon-like activity of the serum. Overlapping the nonspecific phase was one of specific protection, which seemed to be well-established in its own right by day 3 or 4 after vaccination. Serum neutralizing antibodies against the homologous viruses were detected as early as 24 h after vaccination and in almost all instances by day 3.


Effect of dyes on bacterial growth.

Effect of dyes on bacterial growth. A rapid screening procedure was used to test the effect of 42 dyes on growth of 30 bacteria on solid media. The results indicated that many readily available dyes might have potential application for selective isolation of specific bacterial groups as well as value in differentiating between closely related bacterial taxa. Separation of Enterobacter from Escherichia, Salmonella from Shigella, and Staphylococcus from Micrococcus by selected dyes was also evaluated.


Evaluation of potential risk of botulism from seafood cocktails. Clostridium botulinum E could not be detected in 35 samples of commercial seafood cocktails, ranging in pH from 4.10 to 4.85. At 30 C, toxinogenesis in homogenates acidified with a citric-acetic acid mixture was prevented at pH 4.86 or lower for crabmeat and at 5.03 or lower for shrimp. Measurements of the rate of acid penetration into the centers of large pieces of flesh indicated that the already small risk of botulism from seafood cocktails could be completely eliminated by using a cocktail sauce at a maximum pH of 3.70 and by cooling the final product to at least 10 C for 24 h.


Use of transtracheal aspiration in the bacteriological diagnosis of bronchopulmonary infections.


Airway smooth muscle in health and in asthma.


Doxycycline and oxytetracycline in bronchitis: a double blind controlled trial.


A winter-long trial of ampicillin in chronic bronchitis.


Mast cells and allergic asthma.

Mast cells and allergic asthma.


Purification and properties of DNA polymerase 3.


Termination of polypeptide synthesis in bacteria and the exchange of ribosomal subunits.


Kinetics of transcription by the bacteriophage-T3 RNA polymerase in vitro.


In vitro responsiveness of male rat pituitaries of different ages to LH-releasing hormone (LH-RH).


Replication of bacteriophage M-13. Positive role of gene-5 protein in single-strand-DNA synthesis.


In vitro stimulation of LH release by LH-RH in female rat pituitaries of different ages.


Covalent binding of uridine-oligonucleotides to 70 S E. coli ribosomes.


Subunit structure of yeast and rabbit-muscle glucosidase-transferases.


Variation of alkaline phosphatase isoenzymes in Escherichia coli and Serratia marcescens.


Dorsal relaxation incision in burst fingers.


Obituary: Leo Mayer, M.D.

Obituary: Leo Mayer, M.D.


The milk-ejection reflex of the rat: an intermittent function not abolished by surgical levels of anaesthesia.


The milk-ejection reflex of the rat: a 20- to 40-fold acceleration in the firing of paraventricular neurones during oxytocin release.


Ultrastructural preservation of the dense core of posterior pituitary neurosecretory granules and its implications for hormone release.


Tuberculosis control: a special bibliography.


Milk ejection in the rat: the result of a selective release of oxytocin.


Nobel Prize winners in Medicine (1972).

Nobel Prize winners in Medicine (1972).


Incidence of anomalies of central nervous system in Bombay.


Acidic pH and the hepatic detoxification of endotoxin.


Regeneration of hemopoietic stroma in anemic mice of S1-S1 d  and W-W v  genotypes.


Autoradiographic studies in skin homografts.


Pituitary ablation and disseminated prostatic carcinoma.


Medical licensure 1972. Statistical review.


Percutaneous transvenous endomyocardial biopsy.


James Joyce and medicine.

James Joyce and medicine.


Anatomy and the barber-surgeons.

Anatomy and the barber-surgeons.


Group B streptococcal meningitis in adults.


Minnesota Medicine presents Minnesota state medical association roster, 1973.


The effect of ambient temperatures on eating elicited by hypothalamic stimulation.


Alterations in short-term retention, conditioned avoidance response acquisition and motivation following aluminum induced neurofibrillary degeneration.


Erysipelothrix arthritis in pigs as a comparative model for rheumatoid arthritis.


Role of fluphenazine decanoate in lessening the burden of chronic schizophrenics on the community.


Congenital anomalies of the thoracic aorta and its branches: pathophysiology, clinical course, and management.


Enhancement of template activity of calf thymus and rat liver chromatin by  -iirradiation.


Complexes of IgG and IgG rheumatoid factor in synovial tissues of juvenile rheumatoid arthritis.


Noninvasive evaluation of head trauma patients.


Is HMO surgical practice appropriate for academic surgeons?


Pilot study evaluating 1,3-bis(2-chloroethyl)-1-nitrosourea in the treatment of advanced ovarian carcinoma.


Treatment of dissecting aneurysms of the aorta: current status.


Simplified method for the acclerated growth of amniotic fluid cell cultures.


Effects on the neonate of the induction of labor with prostaglandin F 2   and oxytocin.


Classic pages in obstetrics and gynecology: Alexandre Couvelaire.


Morphometric evaluation of pulmonary lesions in rats exposed to ozone. Four hours exposure to 3 ppm ozone produces morphometrically quantifiable alterations in the lungs of rats. Lungs of exposed rats were fixed either immediately after exposure or after a 12-hour recovery period, and the tissue was evaluated using stereologic technics with both the light and electron microscopes. Immediately after exposure, the volume fraction of nonparenchymal tissue was larger (0.057) than for controls (0.045); that of the lumina of large vessels decreased (0.039) from the control value (0.059). The nonparenchymal tissue fraction was 0.053 12 hours later, while the luminal fraction (0.054) resembled the control value. The volume fractions of parenchyma, total tissue and air space showed only slight changes. Ozone also affects all three components of the air-blood barrier, primarily by the production of intracellular edema. The arithmetic mean thickness of the pulmonary blood-air barrier increased from 1.385 +/- 0.095 mu in unexposed rats to 1.700 +/- 0.189 mu in rats immediately after exposure. The primary increase was in thickness of membranous epithelial and capillary endothelial cells. Partial recovery of total barrier thickness (1.582 +/- 0.292 mu) occurred 12 hours after exposure.


Immunohistochemical study of glomerular lesions in retroperitoneal lymphomas. An immunohistochemical study was performed on the kidneys of 10 subjects with retroperitoneal lymphomas. Kidneys were first examined by light microscopy and six kidneys were further studied by immunofluorescence. Light microscopic evidence of damage found in 6 of 10 subjects was characterized by thickening of the basement membrane of glomerular capillaries and an increase in the mesangial matrix. In the areas of light microscopically visible damage, deposits of Schiff-positive material and argyrophilic material were present. No significant changes were found in the tubules. Four of the six kidneys which showed damage by light microscopy showed further evidence of histologic change in areas of glomerular damage when examined using the direct immunofluorescence technic. In 2 subjects, diffuse deposits of IgG and beta1C-beta1A globulin in basement membranes and the mesangial region were prominent, while in the remaining 2 subjects IgG and beta1C-beta1A globulin were localized as linear and/or beaded deposits, both inside and outside the basement membranes. Only focal deposits of these conjugates were seen in the mesangium. No localization of IgA, IgM, rabbit antihuman fibrin or fibrinogen was noted in any of the cases. The tubules yielded negative reactions for the localization of any of the conjugates. The results of this study seem to support the concept that, in some cases of retroperitoneal lymphomas, clinical and renal functional disturbances may be explained on the basis of an immunologic injury to the basement membranes of the glomeruli.


Mode of action of kanamycin on Mycobacterium bovis BCG.


Susceptibility of Mycobacteria to p-nitrobenzoic acid in relation to their niacin production.


The J. Burns Amberson lecture--pulmonary angiitis and granulomatosis.


Variation in lymphocyte reactivity to histoplasmin during the course of chronic pulmonary histoplasmosis.


The physical state of solutes and water in living cells according to the association-induction hypothesis.


Gummatous periostitis in a skull.

Gummatous periostitis in a skull.


Biological availability of digoxin.

Biological availability of digoxin.


Eliot Slater: an appreciation.

Eliot Slater: an appreciation.


Variable dose comparative trial of propranolol and sotalol in angina pectoris.


Organic factors in Gilles de la Tourette's syndrome.


The septum and hyper-reactivity: a review.


Cardiac arrhythmias. 5. Disturbances in cardiac conduction and their management.


The use of an antigen-cocktail in the lymphocyte transformation test.


Assessment of the cellular immune response to HL-A antigens in human renal allograft recipients.


Distribution of microsomal antigen in various types of thyroid tumour.


Lymphocyte binding of aggregated IgG and surface Ig staining in chronic lymphocytic leukaemia.


Computer counting of lithium-treated metaphase chromosomes. A population survey technique.


Ultrastructural morphometry of the pancreatic  -cell.


Hypophysis and function of pancreatic islets. I. The influence of hypophysectomy on insulin secretion and biosynthesis of proinsulin and insulin in isolated pancreatic islets of rats.


Development, differentiation and derivation of the endocrine polypeptide cells of the mouse pancreas. Immunofluorescence, cytochemical and ultrastructural studies.


Role of plasma free fatty acids in the control of insulin secretion in man.


Biological control of snail-borne diseases: a review.


Lithium: useful drug or hazardous teratogen?


Aluminium takes the rap.

Aluminium takes the rap.


Peroperative and postoperative care and monitoring of patients undergoing cardiac surgery.


Pulmonary consequences of abdominal and thoracic surgery.


Physiological aspects of one-lung (endobronchial) anesthesia.


Morphological relations between rat  -secretory granules and the microtubular-microfilament system during sustained insulin release in vitro.


Iowa Medical Society. Officers and committees, 1973-1974.


Antimalarials. 2. Dihydro-1,3-oxazinoquinolines and dihydro-1,3-pyridobenzoxazines.


The incidence of cervico-thoracic spina bifida occulta in South African negroes.


Naphthothiophenes. 2. Benzo(1,2-b:4,3-b')dithiophenemethanols as isosteres of naphthothiophenes.


Synthesis and biological activity of the hypothalamic LH- and FSH-releasing decapeptide.


A conspectus of research on calcium requirements of man.


Comparison of measured and indocyanine green blood flows in various organs and systems.


Historical vignette. Richard Mead, M.D., 1673-1754. 18th-Century exemplar of "experience and reason".


Urinary infection in general practice. Diagnosis and treatment.


Surfactant.

Surfactant.


The care of destitute children in Australia. Beginnings in New South Wales.


Operant conditioning of speech and language in the nonverbal retarded child. Recent advances.


Prevention of retardation of genetic origin.


Psycholinguistics and the evaluation of language function.


A cell and virus disintegrator using an adjustable conical space.


A new method for the preparation of minicells for physiological studies.


Zinc deficiency in acrodermatitis enteropathica: multiple dietary intolerance treated with synthetic diet.


Posture in anaesthesia.

Posture in anaesthesia.


Reticulosarcoma of the vulva complicating dermatomyositis treated by immunosuppression.


Congenital chloridorrhoea.

Congenital chloridorrhoea.


Psychiatry and neurology. Psychosyndrome or brain disease.


Some urological aspects of renal transplantation.


Allergy: immunity gone wrong.

Allergy: immunity gone wrong.


Interrelation between surface tension and mechanical properties of the lung: a historical review.


Computer graphics and cardiovascular data.


Immunological factors and tonsillectomy.


Asbestos cancer: past and future hazards.


Acute anterior uveitis and prostaglandins.


Peripheral problems in proctology.

Peripheral problems in proctology.


New lamps for old.

New lamps for old.


Classification of immunological unresponsiveness and tolerance.


The computer and the clinical signal.

The computer and the clinical signal.


Some methods of deriving cardiac output from central aortic pressure.


Carbohydrate and fluid metabolism in trauma.


Thermoregulation in trauma.

Thermoregulation in trauma.


Endocarditis in slaughter pigs.

Endocarditis in slaughter pigs.


Aetiology and diagnosis of swine dysentery (Doyle).


Paul Langevin.

Paul Langevin.


The five possible causes of all nutrient deficiency: illustrated by deficiencies of vitamin B 12.


Colorimetric assay of liver and Escherichia coli asparatate transcarbamylases in the presence of 2-mercaptoethanol.


A simplified radioassay method of dihydropteroate synthetase activity in Escherichia coli and its application for an inhibition study of p-aminobenzoi  acid derivatives.


Identification of neonatal grey lethal mice.


Human platelet senescence.

Human platelet senescence.


Interspecies patterns of slow virus diseases.


Pulmonary involvement in hypovolemic shock.


Biogenic amines and behavior.

Biogenic amines and behavior.


Staple suturing vs conventional suturing.


Six-month serological observation in a case of A 1 B-like phenomenon.


Successful elution of anti-A and certain anti-H reagents from two "Bombay" (O   A  H ) blood samples and investigation of isoagglutinins in their sera.


Ultrastructure of Shigella and Klebsiella bacteriophages. II. Bacteriophages with noncontractile tails.


The effect of berberine sulfate on healthy mice infected with Candida albicans.


Production mode of systolic clicks due to mitral cusp prolapse.


Aspergillus endocarditis. Therapeutic failure of amphotericin B.


The genetics of hemochromatosis.

The genetics of hemochromatosis.


Hepatitis affecting hemodialysis and transplant patients. Its considerations and consequences.


Liver disease in kidney transplant patients receiving azathioprine.


Urinary  -glucuronidase activity in renal disease.


Parturient paresis. A review.

Parturient paresis. A review.


Pharmacological characteristics of a synthetic releasing hormone LH-FSH-RH (Hoe 471). I. Release of luteinizing hormone.


Talismans and amulets. Vestigial safeguards for 20th century children.


Double-blind study of cyclophosphamide in rheumatoid arthritis.


Hemodialysis and kidney transplantation for renal failure from scleroderma.


Evaluation of the psychoprophylactic method of childbirth in the primigravida.


Institute of Medicine: background and prospects.


D. Clint Smith, M.D., 1934-1973.

D. Clint Smith, M.D., 1934-1973.


Mark T. Sheehan, M.D., 1885-1973.

Mark T. Sheehan, M.D., 1885-1973.


Immune reactions to fractions of crystalline insulin. II. May peri-insulitis be produced by an antigen different from true Sanger insulin?


Immune reactions to fractions of crystalline insulin. I. Significance of lymphocytic infiltrates in the endocrine and exocrine pancreas of mice.


Role of a rigid polyproline spacer inserted between hapten and carrier in the induction of anti-hapten antibodies and delayed hypersensitivity.


Original antigenic sin at the cellular level. II. Specificity of the antibodies produced by individual cells.


In vitro primary response in microcultures containing less than fifty thousand lymphoid cells.


Inhibition of Shigella flexneri by lysates of cells treated with interferon-containing preparations. The in vitro extracellular multiplication of Shigella flexneri is inhibited by cell-free lysates prepared from mouse or human cells pretreated with interferon-containing preparations.


Enhanced immune response after immunosuppression by Streptococcal pyrogenic exotoxin. Streptococcal pyrogenic exotoxin (SPE) was shown previously to be a potent immunosuppressant. This paper presents data documenting that immunosuppression frequently was followed by elevated antibody and plaque-forming cell levels as noted in our earlier report. The data are interpreted as a differential effect of SPE upon suppressor T cells and secreting B cells.


Differences in the response of rabbit small intestine to heat-labile and heat-stable enterotoxins of Escherichia coli. The response of adult rabbit small intestine to the heat-stable (ST) and heat-labile (LT) enterotoxins of Escherichia coli has been investigated by employing the ligated loop technique. Fluid accumulation was determined in relation to enterotoxin dose and duration of gut exposure. The individual responses to ST and LT differed in a characteristic manner. Onset of net fluid accumulation in response to ST appeared to be immediate even at the lowest dose tested. Onset of net fluid accumulation in response to LT was rapid at high doses but delayed at low doses. Maximum volume per length ratios elicited by ST occurred between 4 and 6 h after injection of loops over the entire range of doses tested. However, maximum ratios elicited by LT occurred no less than 10 h after injection even at low doses. Fluid accumulation elicited by LT increased in duration with increasing dose; high doses of LT producing a response which was sustained for at least 18 h. The net effect of these differences in reaction characteristics is a sharp increase in the proportion of the cumulative net secretory response attributable to LT with time. Therefore, a 6-h assay time is appropriate for the titration of ST, whereas an 18-h assay is not. The 18-h assay was found more appropriate for toxin-antitoxin neutralization studies since only LT was neutralized by anti-enterotoxin serum. LT of E. coli (swine) strain P-263 and (human) strain 334 was neutralized by antibody stimulated by enterotoxin from E. coli (human) strain H-10407. LT was labile to mild acid conditions, whereas ST was not.


Prostaglandins and the gastrointestinal tract.


Interferon sensitivity of Venezuelan equine encephalomyelitis virus. Two strains of Venezuelan equine encephalomyelitis virus, which differ in virulence for mice, have been studied for their production of and sensitivity to chick and mouse interferon. Little interferon was produced by chick cells in response to the virulent Trinidad strain or the attenuated TC-83 strain without either aging or priming the cultures. Consistent differences in the production of chick interferon were not found between the two strains. Plaque variants of the Trinidad strain produced higher titers of mouse interferon than the TC-83 strain in both primed and control L-cell cultures. The TC-83 strain was found to be more sensitive than the Trinidad strain to the inhibitory effects of interferon. The greater sensitivity of the TC-83 strain was observed at both high and low multiplicities and for both chick and mouse interferons. These results are consistent with the hypothesis that interferon sensitivity may have a role as a determinant of virulence in some virus-host systems.


Immunoelectrophoretic analysis of Mycoplasma mycoides var. mycoides. Acrylamide gel electrophoresis was used to show the similarities and differences in the membrane proteins of two vaccine and two virulent strains of Mycoplasma mycoides var. mycoides. Immunoelectrophoretic (IEP) analysis was also used to partially characterize the associated antigens. Antibody spectra to the antigens of M. mycoides differ in rabbit, pig, and cattle sera. Rabbits produce better precipitating antibody against the anodic migrating protein mycoplasma antigens than cattle and pigs as seen in IEP. However, rabbit anti-M. mycoides serum did not show precipitating antibody against the heat-stable carbohydrate antigen. As judged by IEP, the major carbohydrate antigen extracted from the media, or boiled whole organism, is similar to that present in the sera-infected cattle and knee joints of calves. This carbohydrate antigen has a cathodic migration in IEP at pH 8.6. Periodate oxidation, classically used to destroy carbohydrate, also destroys most of the protein antigens. Heating the antigens to 56 C for 10 min destroys many of the noncarbohydrate antigens and 100 C eliminates all but the carbohydrate antigen. Extraction of M. mycoides with chloroform-methanol, phenol, ethanol, or ethanol-acetone reduced or eliminated most of the protein antigens. Some of the isolated antigenic fractions of M. mycoides were tested to determine their activity in the diagnostic complement fixation test for contagious bovine pleuropneumonia and their inhibitory effect in this test by using bovine anti-M. mycoides antisera having precipitating antibody and circulating antigen. The complement fixation antigen is not the galactan, cannot be extracted by chloroform-methanol, but is stable to boiling at 100 C and may be extracted by phenol and partially precipitated by ethanol-acetone.


Relationship of some Cryptococcus neoformans hypha-forming strains to standard strains and to other species of yeasts as determined by deoxyribonucleic acid base ratios and homologies. The taxonomic relationship between some hypha-forming Cryptococcus neoformans strains and those that morphologically fit the standard description of this species was determined on the basis of DNA base ratios and DNA-DNA duplex formation. The average guanosine plus cytosine content, as estimated from melting temperature profiles, varied between 43.0 and 45.9% for all C. neoformans strains examined. The relative homology was at least 60% for all C. neoformans strains when the labeled DNA was from either a hyphal variant or a standard strain. The nonpathogenic cryptococci, C. laurentii and C. uniguttulatus, showed less than 10% relative homology with either the hyphal or standard C. neoformans strains. Thus, hyphal and standard strains of C. neoformans were sufficiently related to be considered members of the same species.


Community participation and health resource allocation.


Quantitative immunoelectrophoretic methods as tools for a polyvalent approach to standardization in the immunochemistry of Candida albicans. Quantitative immunoelectrophoresis allows a polyvalent approach to immuno-chemical problems, i.e., identification, quantitation, and thereby standardization of antigens and antibodies in complex mixtures, for example as in crude extracts of microorganisms and their corresponding antisera. This approach is a short cut to conventional standardization of single purified substances, since the polyvalent approach gives a precise quantitative impression as described in this investigation and therefore enables the immunochemist to precisely select the right substance(s) to be purified for further standardization and characterization. To secure the precision of the results, in complex systems it is a sine qua non to select a complex antibody standard, a complex antigen standard, and to describe the complex standard precipitate pattern which again allows a quantitative study of the reproducibility of methods and procedures. The present article describes how such a standardization was approached in this laboratory within the field of Candida albicans immunochemistry. By means of crossed immunoelectrophoresis, 78 water-soluble antigens were demonstrated and enumerated in an antigen standard prepared from one strain of C. albicans A (B 311 Hasenclever). The antibody standard consisted of purified and concentrated rabbit antibodies. The migration velocity of each antigen was indicated in relation to purified human albumin. Not all precipitates could be seen in one immunoplate; therefore a standardized procedure was worked out showing reproducibly 54 precipitates. The reproducibility of quantitation by the crossed-immunoelectrophoresis procedure was determined for each of 30 antigens by repeated measurements; the relative standard deviations ranged from 2.4 to 15.4% and were below 10% for 24 antigens. A simple standardized antigen production procedure was described in great detail, and by quantitative determinations on 30 antigens the procedure was found to be satisfactorily reproducible. By means of crossed-line immunoelectrophoresis in the modification called absorption of antibodies in situ, it was found that strain B 311 contained no specific antigens in comparison to eight other strains of C. albicans. Thirty antigens were quantitated in antigen preparations made by the standardized procedure from the eight strains, and each antigen concentration was expressed as a percentage of the antigen standard. For each antigen a significant inter-strain variation was observed. Of 30 antigens, fifteen were satisfactorily stable after storage at -20 C for 1(1/2) years. The laboratory methods and procedures described in this article thus work with high precision and allow a rapid collection of quantitative data concerning many individual antigens and their corresponding antibodies without purification of antigens. A new complex antigen standard can be made with satisfactory precision from strain B 311. The production of a similar complex antibody standard is a major problem; therefore the main problem in the intra-laboratory standardization seems to be the change from one complex standard to another. In inter-laboratory standardization on the complex level, there seems to be a minimum demand that the first laboratory distributes the antibody standard and that other laboratories use the methods and procedures of the first laboratory.


International comparison of health services systems. An annotated bibliography.


Rodent rabies in the United States.

Rodent rabies in the United States.


Localization of cholera toxin in vivo.

Localization of cholera toxin in vivo.


Mechanism of size limitation of bacterial colonies.


Repair of fractured porcelain denture teeth.


Experimental prostatitis: relationship to pyelonephritis.


Hamish Macmillan.

Hamish Macmillan.


Jack Blomley.

Jack Blomley.


Some problems in the treatment of fractures of the shaft of the tibia and fibula.


[Prophage induction and cell division in E. coli. II. Linked (recA, zab) and unlinked (lex) suppressors of tif-1-mediated induction and filamentation].


Isolation and genetic localization of three phi-X174 promoter regions.


Mutants of Escherichia coli with a defect in the degradation of nonsense fragments.


Isolation of membrane proteins.

Isolation of membrane proteins.


Characteristics of pentazocine dependence in hospitalized patients after naloxone administration.


Genetic polymorphism.

Genetic polymorphism.


Research into things past: tracking down one of Miss Nightingale's correspondents.


New members of the New York Academy of Sciences.


The uptake and destruction of Salmonella typhimurium by some body tissues of sheep.


Activity of sulphachloropyridazine aganist experimentally induced E. coli infection in broiler chickens.


Artificial rearing of lambs: a review.

Artificial rearing of lambs: a review.


Experimental production of aneurysms by microvascular surgery in rabbits.


Historical note on inheritance of sickle cell anemia.


Phenotypic diversity of human diseases resulting from allelic series.


Utilization and costs of psychiatric care: key issues in the coming debate on national health insurance.


The grief of soldiers: Vietnam combat veterans' self-help movement.


Social psychiatry: an overview.

Social psychiatry: an overview.


Utilization review of psychiatric inpatient care.


If they were here now.

If they were here now.


Drug comparison in hyperactive children.


Methylphenidate in tardive dyskinesia.

Methylphenidate in tardive dyskinesia.


Recovery of bacteriophate from contaminated chilled and frozen samples of edible West Coast crabs. Edible West Coast crabs (Cancer magister and C. antennarius) were contaminated with bacteriophage and then held in a chilled or frozen state. Results indicated a significant survival of virus regardless of storage conditions.


Automated detection of Haemophilus influenzae. Addition of heme (X factor) and pyridine nucleotide (V factor) to the medium permits rapid growth of Haemophilus influenzae, with evolution of easily detectable amounts of (14)CO(2). Radiometric media containing X and V factor should be used when evaluating clinical specimens which might contain Haemophilus species.


Mycobacterium microti (vole bacillus): a method for viable counts within 21 days of culture. A method is described for counting viable units of Mycobacterium microti (vole bacillus) suspensions after 21 days of culture on an oleic-albumin agar medium containing 5% defibrinated horse blood, with a tight seal to maintain humidity. This method is important because vole bacillus is an alternative to BCG in the prevention of tuberculosis.


Some factors affecting the activity of diethylpyrocarbonate as a sterilant. Quantitative data indicated logarithmic death in 5 degrees Brix Concord grape juice when concentrations of cells under 10(7)/ml were exposed to diethylpyrocarbonate (DEPC). Species differed considerably in their resistance; e.g., 50 ppm reduced the viable count of Saccharomyces cerevisiae over nine log(10) cycles, whereas 200 ppm reduced the count of Byssochlamys fulva ascospores by only about 1 log. DEPC lethality was enhanced by higher temperatures; destruction at 40 C was 10- to 100-fold greater than at 20 C. Studies on death rates showed that most yeasts and fungal spores were killed during the first hour of exposure, whereas 24 h or longer was needed for maximal destruction of several lactic acid bacteria. Repair of DEPC-induced damage was believed responsible for the slower death rates of the lactics.


Microbiological evaluation of cold-water shrimp (Pandalus borealis). A bacteriological survey of the Maine shrimp industry was conducted to investigate the conditions associated with the production of frozen, raw, peeled shrimp. In-plant samples and finished product units were collected from seven plants. The most probable number of Escherichia coli, coliforms, and coagulase-positive staphylococci, as well as aerobic plate counts (APC), were determined. Freshly harvested shrimp collected from fishing vessels had an APC geometric mean of 510/g, and E. coli, coliforms, and coagulase-positive staphylococci were absent. Subsequent storage and insanitary practices during processing increased the APC and introduced coliforms. However, the low air temperatures (18 to 45 F) in the plants and the large volumes of cold water (34 F) used during processing inhibited significant bacterial buildup in the finished product.


Production of a sporulation pigment by Streptomyces venezuelae. Streptomyces venezuelae S13 produced a pH-indicating sporulation pigment on a glucose-salts-agar medium consisting of glucose, KNO(3), MgSO(4), and Na(2)HPO(4), pH 7. Pigmentation on this medium appeared to be closely associated with sporulation, which normally required 5 to 7 days at 30 C. The pigment was soluble in water as well as in a number of organic solvents. Butanol-extracted pigment exhibited absorption maxima at 430 and 520 nm at pH 3 and 12, respectively. Although many salts of organic acids and amino acids could replace glucose as the sole carbon source in basal salts-agar medium for growth and pigmentation, most sugars that were tested supported good growth but negligible pigmentation. Among the nitrogenous substances tested, KNO(3) was most desirable for pigmentation. The organism did not exhibit any specific requirements for divalent cations with respect to growth and pigmentation. In the absence of MgSO(4), however, glucose-salts-agar prepared by autoclaving all components together failed to support growth. The production of the sporulation pigment on glucose-salts-agar was comparable to that obtained on tomato paste-oatmeal-agar medium. Incorporation of partially purified pigment material into broth medium that did not normally support sporulation induced sporulation, and amino acid-salts-agar medium could induce vegetative mycelia to pigment when transferred from medium that did not support either pigmentation or sporulation.


Microbial formation of nitrosamines in vitro. Mortierella parvispora and an unidentified bacterium converted trimethylamine to dimethylamine, and the bacterium (but not the fungus) formed dimethylnitrosamine in the presence of nitrite. Dimethylnitrosamine also appeared in cell suspensions of Escherichia coli and Streptococcus epidermidis and in hyphal mats of Aspergillus oryzae incubated with dimethylamine and nitrate. Suspensions of a number of microorganisms produced N-nitrosodiphenylamine from diphenylamine and nitrite at pH 7.5, and soluble enzymes catalyzing the N-nitrosation of diphenylamine were obtained from two of these organisms. In the presence of these enzymes, several dialkylamines were converted to the corresponding N-nitroso compounds.


Renal function in respiratory failure.

Renal function in respiratory failure.


Potential pathogens in the environment: Klebsiella pneumoniae, a taxonomic and ecological enigma. A nitrogen-deficient medium and m-Endo agar were employed in the isolation of members of the tribe Klebsielleae from surfaces of vegetables and seeds. With m-Endo agar at an incubation temperature of 37 C, nearly 50% of the vegetables and seven out of seven seed samples yielded organisms which biochemically and serologically were identified as Klebsiella pneumoniae, Viable counts were generally in the range of 10(3) cells per g of vegetable peel or seed. Organisms classified as K. pneumoniae exhibited seven different IMViC patterns, with the --++, ++++, and -+++ patterns most common. Seven of the eleven K. pneumoniae serotypes encountered have previously been isolated from human urinary tract and other infections. Fifty percent of the 40 K. pneumoniae examined exhibited positive acetylene-reducing activity, i.e., they possessed the capability for fixing N(2). Vegetables containing K. pneumoniae may constitute a potential reservoir for human nosocomial genitourinary or other infections.


Clinical laboratory experience with the improved Enterotube. The reactions elicited in this evaluation of the improved Enterotube indicate the efficacy of using this device for the differentiation of the members of the family Enterobacteriaceae.


Effect of temperature of incubation on performance of media in the detection of enteric pathogens. The effect of incubation temperatures on the efficacies of both plating media and transport or enrichment broths was determined by the analysis of 391 diarrheal stools for salmonellae and shigellae. Each analysis resulted in 90 observations. Stool specimens were homogenized in saline and used to inoculate eosin methylene blue (EMB), Salmonella-Shigella (SS), and xylose lysine deoxycholate (XLD) agar plates, Amies and Cary-Blair (CB) transport media, and gram-negative (GN) enrichment broth. All media were incubated at 25, 30, and 35 C for 24 and 48 h. In order of efficacy, GN and saline were significantly better than Amies and CB, which were still better than direct streaking for both salmonellae and shigellae. Forty-eight hours was a significant improvement over 24 h only at 25 C on direct streaking for both pathogens. Salmonella detection was also improved at 30 over 25 C on direct streaking. In direct plating, XLD was better than both SS and EMB for both pathogens. After broths, for salmonellae, XLD > SS > EMB, and for shigellae, XLD > EMB > SS, with all differences significant. SS agar was significantly improved for detection of shigellae with 48-h broth inocula versus 24-h broth inocula. The differences thus observed at the various temperatures tested proved to be less important than the media used. The efficient media, GN broth, saline-stool, and XLD were shown to be affected very little by either temperature or time variance of the magnitude tested.


In memoriam, 1906-1973 (Abraham J. Gitlitz).


Negative chemotaxis of marine bacteria to toxic chemicals. The positive chemotactic responses of motile marine bacteria were reversed by the addition of sublethal concentrations of a toxic chemical. The negative chemotactic effect was observed with a wide range of toxicants including heavy metals and hydrocarbons. This phenomenon may be utilized to develop new approaches for control of marine fouling.


Bacterial respiratory infections: a review of the problems of etiology and antimicrobial susceptibility testing.


Identification of the Prototheca species by immunofluorescence. Studies were carried out to develop fluorescent antibody reagents for the identification of the Prototheca species and for their differentiation from morphologically similar fungi of various genera in formalin-fixed tissues. Antisera against representative isolates of P. filamenta, P. moriformis, P. stagnora, P. wickerhamii, and P. zopfii were produced in rabbits. Antiglobulins, labeled with fluorescein-isothiocyanate that intensely stained most cells of the homologous species, were selected for use as potential diagnostic reagents. By adsorbing the conjugates with selected heterologous cross-staining protothecae, reagents that were both sensitive and specific were obtained. Evaluation of the adsorbed conjugates with sections of tissue infected with protothecae, sections of tissue infected with morphologically similar fungi, and cultures of protothecae showed that these reagents are useful for the rapid and reliable identification of the Prototheca species.


HL-A antigens in pemphigus.

HL-A antigens in pemphigus.


Azaribine in the treatment of psoriasis. A low dose, double-blind evaluation.


Familial occurrence of pemphigus foliaceus.


Erosio interdigitalis blastomycetica.

Erosio interdigitalis blastomycetica.


Experimental infection with Candida albicans.


Carbenoxolone in the treatment of duodenal ulcer. A multicentre trial.


A double-blind comparison of pimozide and haloperidol in the treatment of recurrent anxiety states.


Topical ampicillin and noxythiolin antisepsis in appendicectomy wounds.


The speed tray technic when saving time is important.


Sinus histiocytosis in non-diagnostic scalene lymph node biopsies.


Complete remissions of mycosis fungoides lymphoma induced by topical nitrogen mustard (HN2). Control of delayed hypersensitivity to HN2 by desensitization and by induction of specific immunologic tolerance.


The schizophrenic 'artist's' expression of movement.


Antigenic competition.

Antigenic competition.


Antibodies to receptors recognizing histocompatibility antigens.


Genetics of the F sex factor in enterobacteriaceae.


Design and construction of partial dentures externally retained. I.


Stacking interactions in chemically modified tRNA's and oligonucleotides.


Isolation of E. coli R factor DNA by molecular sieve chromatography in 4 M guanidine hydrochloride.


Formation of complexes between tRNA and trinucleotides.


Two dimensional distribution of concanavalin-A receptor molecules on fibroblast and lymphocyte plasma membranes.


Superoxide dismutase in baker's yeast.

Superoxide dismutase in baker's yeast.


The case against the blighted potato.

The case against the blighted potato.


Metabolism as a factor in determining the toxic action of the aflatoxins in different animal species.


Polymers in surgical repair.

Polymers in surgical repair.


Organosiloxanes and reproduction.

Organosiloxanes and reproduction.


The occurrence of vesicoureteral reflux and pyelonephritis in apparently normal dogs.


An investigation of the relationship of monsepecific urethritis corynebacteria to the other micoorganisms found in the urogenital tract by means of modified chocolate agar medium.


Erastus B. Wolcott, M.D.

Erastus B. Wolcott, M.D.


Scanning electron microscopy of human dermal fibrous tissue.


Current status of bonding attachments.

Current status of bonding attachments.


Rapid palatal expansion cases.

Rapid palatal expansion cases.


Lymphoglandular complexes in the large intestine of the dog.


Greater Kansas City Dental Society 1973 membership directory.


On the regulation of D-ribose metabolism in E. coli B-r. I. Isolation and characterization of D-ribokinaseless and D-ribose permeaseless mutants.


On the regulation of D-ribose metabolism in E. coli B-r. II. Chromosomal location and fine structure analysis of the D-ribose permease and D-ribokinase structural genes by P 1  transduction.


Degradation of RNA in Escherichia coli. A hypothesis.


Genetic studies with two prophages naturally resident in Serratia marcescens HY.


Retention of cytoplasmic killer determinants in yeast cells after removal of mitochondrial DNA by ethidium bromide.


Phage and prophage decision in defective lambda-lysogens: the role of cII product.


Demonstration of antibodies against 7,12-dimenthylbenz(a)anthracene-induced rat mammary tumors by immunofluorescence.


Studies on schistosomiasis. 3. Detection of antibodies against Schistosoma mattheei by the indirect immuno-fluorescent method.


Molecular control mechanisms in muscle contraction.


Iron absorption.

Iron absorption.


Transannular peroxides as radiation sensitizers.


Radioprotection of enzyme and bacterial systems by dithiothreitol.


Allelic interaction at the rad 1 locus in diploid cultures of UV-irradiated yeast.


Hematopoietic CFU in mice infected by the polycythemia-inducing Friend virus. II. Survival of lethally irradiated mice grafted with spleen or bone marrow cells from infected donors.


Carnauba wax as a hardening agent for paraffin embedding mixtures.


Epidemiological and clinical aspects of sudden cardiac death.


Smoke and smoking in British bronchitis.


Multiple sclerosis in migrant populations.


International variations in psychiatric diagnosis.


Glucose intolerance and cardiovascular disease.


Salt and hypertension, water hardness and cardiovascular death rate.


Members of the Virginia Dental Association.


Carbenoxolone in the treatment of gastric ulcer.


Review of carbenoxolone: editorial comment.


Conformational change of pepsin induced by its substrate N-acetyl-L-phenylalanyl-L-3,5-diiodotyrosine.


Mitochondriogenesis in synchronous cultures of yeast. I. Oscillatory pattern of respiration.


Replication of RNA viruses: analysis by liquid polymer phase partition of the binding of Q  RNA polymerase to nucleic acid.


Effect of eukaryote DNA on amino acid incorporation in extracts of E. coli.


Immunoferritin studies of Marek's disease virus directed intracellular and membrane antigens.


Intracellular localization of mannan synthetase activity in budding baker's yeast.


Formation of D-1-amino-2-propanol from L-threonine by enzymes from Escherichia coli K-12.


Biosynthesis of the luteinizing hormone releasing hormone in mitochondrial preparations and by a possible pantetheine-template mechanism.


Non-chromosomal respiratory deficient mutants induced by guanidine hydrochloride in Saccharomyces cerevisiae.


Participation of the tryptophan synthase inactivating system from yeast in the activation of chitin synthase.


Localization of the structural gene for the  ' subunit of RNA polymerase in Escherichia coli.


Alterations in plasma and brain lipid metabolism during endotoxemia in the neonatal rat.


Frederick Mott, founder of the Maudsley Laboratories.


The use of clustering techniques for the classification of psychiatric patients.


Behavioural studies of the actions of cocaine, monoamine oxidase inhibitors and iminodibenzyl compounds on central dopamine neurones. 1. Turning behaviour after unilateral lesions of the nigro-striatal dopamine pathway in rats has been used to compare the actions of cocaine and desipramine on central dopamine-containing neurones.2. Administration of cocaine alone (5-20 mg/kg) resulted in no turning or minimal turning towards the lesioned side; the monoamine oxidase inhibitors nialamide and pargyline administered alone were also ineffective.3. After pre-treatment with nialamide (100 mg/kg) or pargyline (25 mg/kg) cocaine evoked high rates of turning towards the lesioned side.4. Desipramine (1-100 mg/kg), either alone or in combination with nialamide did not evoke turning.5. Turning evoked by the cocaine-nialamide combination was abolished by pre-treatment with alpha-methyl-p-tyrosine (150 mg/kg).6. Pre-treatment with reserpine (5 mg/kg, 24 h previously) substantially diminished turning evoked by the cocaine-nialamide combination but potentiated turning resulting from administration of methylamphetamine (5 mg/kg).7. Cocaine (20 mg/kg) administered 15 min prior to (+)-methylamphetamine (5 mg/kg) reduced the turning behaviour in the first hour after administration of the latter drug but prolonged the total duration of the effect.


Propranolol in neurotic and thyrotoxic anxiety.


Ten years' experience in the surgical treatment of basal-cell carcinoma. A study of factors associated with recurrence.


Anorectal suppuration: the results of treatment and the factors influencing the recurrence rate.


Renovascular hypertension: the place of renal autotransplantation.


Omental transposition in the treatment of radionecrosis.


Production and assay of dog, pig and goat heterologous anti-rabbit lymphocyte serum.


Radiation and pregnancy.

Radiation and pregnancy. Irradiation during pregnancy may occur either as the result of radioactive pollution of the environment, or during a medical procedure using x-rays or radionuclides. While the former is usually unforeseeable, the latter is known and accepted by both physician and patient.Recent statistics estimate that about one quarter of pregnant women have had a radiographic experience during the pregnancy, either for obstetrical reasons or in the course of medical and dental examinations. The amount of radiation delivered to the fetus is in the range of one rad or less. Radionuclidic procedures may result in fetal radiocontamination, chiefly after placental transfer and fetal uptake. Radioiodine, radioactive calcium and selenomethionine are dangerous for the fetus, since they cross the placenta freely and are taken up by fetal tissues. The labelled proteins, radiocolloids and some mercury compounds remain in the maternal compartment and therefore can affect the fetus only through their gamma radiation at some distance from the fetus.The teratogenic effect, the leukemogenic threshold and the lowered resistance to neonatal infections have been demonstrated after irradiation with doses far higher than those encountered during diagnostic applications of ionizing radiation. Statistical data suggest an increase of susceptibility to leukemia in infancy after intra-uterine irradiation at a diagnostic level. Cytogenic analysis may.... offer valuable data for the establishment of the extent of radiation damage.


The intestinal tract in relation to obstetrics and gynecology. Operative technique.