Mechanism of inhibition of deoxyribonucleic acid synthesis in Escherichia coli by hydroxyurea. The effects of hydroxyurea on Escherichia coli B/5 physiology (increases in cell mass, number of viable cells, and deoxyribonucleic acid [DNA], RNA, and protein concentrations) were studied in an attempt to find a concentration that completely inhibits DNA synthesis and increase in number of viable cells but has little or no effect on other metabolic processes. These conditions were the most closely approached at an hydroxyurea concentration of 0.026 to 0.033 m. A concentration of 0.026 or 0.033 m was used in subsequent experiments to study the site(s) of inhibition of DNA synthesis in E. coli B/5 by hydroxyurea. Hydroxyurea at a concentration of 10(-2)m was found to inhibit ribonucleoside diphosphate reductase activity completely in crude extracts of E. coli. The synthesis of deoxyribonucleotides was greatly reduced when E. coli cells were grown in the presence of 0.033 m hydroxyurea. Studies on the acid-soluble DNA precursor pools showed that hydroxyurea causes a decrease in the concentration of deoxyribonucleoside diphosphates and deoxyribonucleoside triphosphates and an increase in the total concentration of ribonucleotides. Sucrose density gradient sedimentation of DNA from cells treated with 0.026 m hydroxyurea for 30 min indicated that at this concentration hydroxyurea induces no detectable single- or double-strand breaks. In addition, both replicative and repair syntheses of DNA were found to occur normally in toluene-treated cells in the presence of relatively high concentrations of hydroxyurea. Pulse-chase studies showed that deoxyribonucleotides synthesized prior to the addition of hydroxyurea to cells are utilized normally for DNA synthesis in the presence of hydroxyurea. On the basis of these observations, we have concluded that the primary, if not the only, site of inhibition of DNA synthesis in E. coli B/5 by low concentrations of hydroxyurea is the inhibition of the enzyme ribonucleoside diphosphate reductase.


Location of acid phosphatase and  -fructofuranosidase within yeast cell envelopes. After 16 hr of incubation in a low-phosphate, aerated medium, bakers' yeast was obtained with a high titer of acid phosphatase (EC 3.1.3.2) and beta-fructofuranosidase (EC 3.2.1.26). All of the beta-fructofuranosidase and 75% of the acid phosphatase were easily released by mechanical disruption in a French pressure cell. The cell wall suffered a limited number of cracks, but this was sufficient for the co-release of these enzymes. Both enzymes were subject to autolytic release, although correlation was inconclusive because of the relative instability of acid phosphatase. The data are consistent with the bulk of the two enzymes being located in the periplasmic space. Ethylacetate treatments yielded ghosts with high beta-fructofuranosidase but low acid phosphatase activities. The surviving acid phosphatase was not representative of that in live cells. It was resistant to release by mechanical disruption and showed a high susceptibility to heat inactivation. The beta-fructofuranosidase in live cells and in ethylacetatetreated cells exhibited polydispersity in heat inactivation susceptibility; but the kinetics were indistinguishable, and facile release by mechanical disruption was shown in both cases.


Structural changes associated with extraction of group E spore antigen of Clostridium botulinum. Spores of type E Beluga strain of Clostridium botulinum, which were hydrolyzed with HCl to remove the spore-specific antigen, showed a spore coat which was thinner and projected into the exosporium with multiple protuberances. The changes observed suggest that the antigen is a component of the spore coat connected with the maintenance of rigidity.


Relation between protein synthesis and phospholipid synthesis and turnover in Escherichia coli. Turnover of phospholipids occurred continuously in a fatty acid auxotroph of Escherichia coli, but in the wild-type parent strain significant turnover occurred only under conditions where protein synthesis was inhibited but lipid synthesis continued. Both strains gave a stringent response of ribonucleic acid accumulation to amino acid starvation, but only in the wild type was lipid synthesis also inhibited. A revertant strain of the auxotroph resembled the wild type in this respect. The phospholipid that accumulates in the culture medium as a result of the lipid turnover appears to be part of a loosely bound low-density complex arising from the cell envelope.


Formation of polyisoprenol phosphate glucose in Shigella flexneri. Lipid-linked glucose is formed from uridine diphosphate-glucose and ficaprenol phosphate only in strains of Shigella flexneri with glucosylated O antigen.


Pattern of replication of a colicin factor during the cell cycle of Escherichia coli. The ColBM, trp, lac episome was transferred to a lacZ derivative of Escherichia coli B/r, and the manner of replication of this colicinogenic factor was followed through the cell cycle. The results suggest a pattern of replication not connected with any particular stage in the cell cycle.


Location of the region controlling host specificity (hsII) with respect to drug resistance markers on the fi -  R factor, N-3. The region controlling host specificity (hsII) has been mapped, by P22 transduction, with respect to drug resistance markers carried on the fi(-) R factor, N-3. The relative linear sequence of cotransducible markers is Tc-Su-Sm-hsII.


Deoxyribonucleic acid replication and cell division in Escherichia coli at 33 C. At 33 C (60-min generation time) the time required to replicate the chromosome is C = 60 min. The time between the end of a round of replication and cell division is D = 20 min, as at 37 C. Nalidixic acid and a temperature shift in a dnaB mutant give identical results for the determination of the end of a round of replication.


"Periplasmic" location of a  -lactamase specified either by a plasmid or a chromosomal gene in Escherichia coli. Type IIIa beta-lactamase (TEM beta-lactamase) was "periplasmic" in Escherichia coli K-12 regardless of whether its gene was chromosomal or carried on an R factor.


Inhibition of thiamine transport by chloroethylthiamine in Escherichia coli. Chloroethylthiamine was found to inhibit an entrapment of thiamine as thiamine monophosphate by blocking thiamine monophosphokinase in the cytoplasm after thiamine was taken up by the cells of Escherichia coli.


A statistical approach to the polymorphonuclear leukocyte bactericidal assay.


Histochemical and biochemical studies of urinary lipids in metachromatic leukodystrophy and Fabry's disease.


Biotransformation of 1,4-dihydro-2,6-dimethyl-4-(2-trifluoromethylphenyl)-3,5-pyridinedicarboxylic acid diethyl ester.


Paul Clifford Kitchin, 1891-1972. President of the IADR, 1937-1938.


Antimalarials. 4. 4-Pyridinemethanols with styryl and benzoyl substituents.


Antimalarials. Some 9-substituted amino-6-chloro-2-methoxyacridines.


The use of blade implants in a selected population of partially edentulous patients; a preliminary report.


The subcutaneous implantation of periodontally diseased roots.


Heats of adsorption of ammonia and carbon dioxide on tooth components.


Aphthous ulceration: the antinuclear factor.


Variable tissue forces produced by water jet devices.


Bonding of resins to phosphoric acid-etched enamel surfaces of permanent and deciduous teeth.


Effect of eugenol and eugenol-containing root canal sealers on the microhardness of human dentin.


Plication sutures for prevention of scrotal haematoma.


A simple and inexpensive container for the cultivation of anaerobic microorganisms.


The organization of personal health services.


Some accomplishments and findings of neighborhood health centers.


Acute ulcerative gingivitis: immune complex.


Apicoectomy and the crucial abutment tooth.


Action being taken against denturists in Ontario.


Management of midfacial fractures.

Management of midfacial fractures.


A clinical evaluation of fentanyl for outpatient sedation in dentistry.


Wegener's granulomatosis presenting as gingivitis. Review of the clinical and pathologic features and report of a case.


The occurrence of a verruca vulgaris on an intraoral skin graft. A unique growth with significant implications.


A comparison of the management of pulpal pathosis in deciduous and permanent teeth.


The course of bone gain and the phases of bone loss.


Osteoporosis in rheumatoid arthritis and corticosteroid induced osteoporosis.


Pathophysiology, diagnosis and treatment of osteomalacia.


Renal osteodystrophy.

Renal osteodystrophy.


The bony changes in malignant hematologic disease.


Advances in the management of fractures and dislocations in the past decade.


Geoffrey Gossip Harkness.

Geoffrey Gossip Harkness.


The effect of prednisone on serum cholesterol in patients with cirrhosis of the liver.


An American view of BCG vaccination, illustrated by results of a controlled trial in Puerto Rico.


In vivo and in vitro studies on alpha-receptors in human airways. Potentiation with bacterial endotoxin.


A theoretical study on flow patterns of ventilators.


Pneumocystis carinii pneumonia in Norway.


Fast and slow setting functional impression materials used in connection with complete denture relinings.


Studies on the bacteriophage MS2. XX. Expansion of the virion in low salt.


Formation of various genome lengths of hybrids between the serologically and morphologically unrelated bacteriophage species.


Interference in phage growth by a resident plasmid lambda dv. I. The mode of interference.


Less tumorigenic (cyt) mutants of adenovirus 12 defective in induction of cell surface change.


The fate of pea enation mosaic virus in its pea aphid vector, Acyrthosiphon pisum (Harris).


Intracellular events in canavanine-treated, T4-infected Escherichia coli.


Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. I. Transduction of R factor 222 by phage P22.


Synchronous growth of Escherichia coli C 600 dependent on a lambdoid prophage.


Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. II. Properties of a high-frequency-transducing lysate.


Hypothalamic control of prolactin secretion.


Comparative endocrinology of gestation.

Comparative endocrinology of gestation.


Effect of vitamin E deficiency on cellular membranes.


FSH-releasing hormone and LH-releasing hormone.


Availability of iron dextran for hemoglobin synthesis as studied with phlebotomy.


Availability of iron sorbitol for hemoglobin formation as studied with phlebotomy.


Blood pressure, heart rate and respiratory responses from hypothalamic self-stimulation in the cat.


Inhibitory effect of glycerin on Vibrio parahaemolyticus and Salmonella. In a study of the effect of glycerin in transport media on Vibrio parahaemolyticus and Salmonella, it was found that a concentration of 30% glycerin was highly inhibitory for V. parahaemolyticus and to a lesser degree for Salmonella. The incorporation of peptone or human feces in media did not reduce the inhibitory effect of glycerin. In media with 15% glycerin, viable counts of V. parahaemolyticus and Salmonella increased after 24 hr of incubation both in the presence and absence of feces. Due to the concurrent increase in the total bacterial count in the media containing feces, no enrichment effect was noted.


Isolation and identification of psychrophilic species of Clostridium from milk. Four of 48 raw milk samples contained catalase-negative, gram-positive, motile, sporeforming, rod-shaped bacteria that grew optimally at 22 to 30 C and slowly at low temperatures. Isolates from two samples had a minimal growth temperature of 4 C, were anaerobic, and had characteristics similar to Clostridium hastiforme; those from the other two samples had a minimal growth temperature of 0 +/- 1 C, were anaerobic, aerotolerant, and had characteristics similar to C. carnis. Specific growth rates, doubling times, ability to grow in pasteurized milk stored in commercial cartons, and resistance of spores to heating were determined for one strain of C. hastiforme.


Validity of spinal cord examination as a substitute procedure for routine rabies diagnosis. A significant portion of specimens received by this laboratory for rabies diagnosis is unsatisfactory for testing due to decomposition of the brain, or severe mutilation of the head when the animal was killed. Examination of the spinal cord was therefore explored as a possible alternative method when standard brain examination was not possible. In this study, both brain and spinal cord of 248 rabies-suspect animals were examined to assess the reliability of the spinal cord method. Brain and spinal cord of the 248 animals were examined by fluorescent antibody (FA) method, and mouse inoculation tests were performed on 247 brain specimens and 13 spinal cord specimens. By using both brain and spinal cord, 30 animals representing 8 species were diagnosed as rabid by FA, and 218, representing 11 species, were negative. There was 100% agreement between two procedures with FA as the criterion. This study showed that in cases where the usual examination is precluded due to brain destruction, the spinal cord procedure offers an equally reliable alternate method of diagnosis.


An unclassified gram-negative rod isolated from the pharynx on Thayer-Martin medium (selective agar). An oxidase-positive, small gram-negative rod was isolated on Thayer-Martin medium (TM) inoculated with pharyngeal swabs obtained during surveys to detect Neisseria carriers. In one survey, this organism was isolated from 48% of the subjects, and 50 or more colonies were present on the majority of the primary isolation plates. Other characteristics of the organism, which has been given the provisional designation "TM-1," include: delayed production (2 to 10 days) of acid from glucose, formation of gas during nitrate reduction, and the frequent formation of "pits" in the agar surface. On TM, nonpitting colonies of TM-1 are morphologically similar to colonies of Neisseria gonorrhoeae and N. lactamica. Comparison of the characteristics of TM-1 strains with other similar fastidious gram-negative organisms encountered in clinical laboratories indicates that TM-1 is a distinct species. Further studies are required before proper taxonomic placement can be made.


Cinemicrographic study of the development of subsurface colonies of staphylococcus aureus in soft agar. Cinemicrographic studies revealed that the development of elongated subsurface colonies of Staphylococcus aureus in soft agar (<0.2% agar) originated with a colony-forming unit of about 10 to 20 cells. It was then observed that small clusters of 3 to 12 cells broke off from the main colony unit and drifted away under the combined influence of gravity and Brownian motion. Once the downward or slightly sideward motion of the small clusters ceased, the clusters would continue to increase in size; at the same time, additional small clusters broke off, and the cycle was repeated until the entire colony was formed. Displacement and velocity measurements were made on the drifting small clusters. When compared with the dimensional growth rate and geometry of the subsurface colony, these showed that a correlation existed between the movement and velocity of the small clusters and the subsequent colony development. A relationship between the role of gravity reported in these results and the development of spherical colonies after rotation on a clinostat is suggested.


Pseudomonas putrefaciens isolates from clinical specimens. A total of 109 cultures of Pseudomonas putrefaciens isolated from clinical specimens were studied. The cultures were separated into two groups. The majority of the group 1 isolates, comprising 31 cultures, were characterized by (i) growth in plain nutrient broth, but no growth in broth supplemented with NaCl at concentrations of 7% and above, (ii) no growth on Salmonella-Shigella (SS) agar, and (iii) production of acid from the carbohydrates, sucrose, maltose, arabinose, and dextrin. Most group 2 isolates, comprising 78 cultures, were (i) unable to grow in plain nutrient broth, but grew well in broth supplemented with NaCl at a concentration of 7 to 10%, (ii) able to grow on SS agar, and (iii) unable to produce detectable amounts of acid from any of the carbohydrates tested except for variable results with glucose and fructose.


Survival of coliform bacteria in natural waters: field and laboratory studies with membrane-filter chambers. Chambers with membrane-filter side walls were designed for studies of the survival of coliform bacteria in natural and artificial waters. Experiments were carried out in the field and in the laboratory. The initial uptake rate of inorganic ions, total carbon, and glucose into the chamber was greater than twice as fast as the accumulation of each into dialysis tubing. When the survival of a water-isolated fecal coliform bacterium was examined in two adjacent mountain streams, it was found that the organism persisted longer in Bozeman Creek than in Middle Creek. These data may be a reflection of the water chemistry because the concentration of inorganic constituents of the former was greater. Laboratory studies of the survival of a fecal coliform bacterium in artificial and natural water with continuous flow were used to determine the effect of chemical composition, temperature, and pH. The relation of this type of data to the use of fecal coliform bacteria as indicators of health hazard in water is discussed.


The role of micellar solubilization in lipid absorption.


A method for maintaining the viability of Vibrio fetus var. venerealis in samples of preputial secretions collected from carrier bulls.


High-voltage electron microscopy of wet whole cancer and normal cells. Visualization of cytoplasmic structures and surface projections.


Isolation and characterization of an Escherichia coli mutant with alteration in the outer membrane porteins of the cell envelope.


The mechanism of inhibition of anaerobic phosphate uptake by fatty acids in yeast.


Studies on the biological properties of polyene antibiotics: comparison of other polyenes with filipin in their ability to interact specifically with sterol.


Gastric motor activity during feeding induced by stimulation of the lateral hypothalamus in the rat.


Ambivalence in rats using single-lever Skinner boxes.


The earliest medical use of the caduceus.


Bone marrow precursor cells specify serological properties of antibody.


In vitro sensitization to transplantation antigens. V. Suppression of T-cell and of B-cell function in vitro.


Direct demonstration of lymphocyte--macrophage cooperation in the absence of physical contact between the two cell types.


Heteroclitic cells in anti-hapten systems: some studies at the cellular and serum level.


The immunologic effect of chlorphenesin on skin grafts in normal and thymectomized mice.


Freeze-cleaved replicas of rabbit vitreous.


Some recent developments in human tumour immunology.


Mechanisms of active transport in isolated bacterial membrane vesicles. X. Inactivation of D-lactate dehydrogenase and D-lactate dehydrogenase-coupled transport in Escherichia coli membrane vesicles by an acetylenic substrate.


Identification of a glutamic acid at the active center of bovine carboxypeptidase B.


Structure and role of carbohydrate in freezing point-depressing glycoproteins from an antarctic fish.


The developmental biochemistry of cotton seed embryogenesis and germination. IV. Levels of cytoplasmic and chloroplastic transfer ribonucleic acid species.


Studies on the structure of T4 thioredoxin. I. Isolation and amino acid sequence of the peptides obtained from a tryptic digest of the protein.


Acetyl coenzyme A carboxylase. Molecular forms and subunit composition of biotin carboxyl carrier protein.


Steroid binding properties of some peptide fragments of bovine serum albumin obtained on peptic digestion.


Studies on the structure of T4 thioredoxin. II. Amino acid sequence of the protein and comparison with thioredoxin from Escherichia coli.


Peptide synthesis by prokaryotic-eukaryotic hybrid ribosomes.


Succinyl coenzyme A synthetase of Escherichia coli. Effects of phosphoenzyme formation and of substrate binding on the reactivity and stability of the enzyme.


The assembly of a structural lipoprotein in the envelope of Escherichia coli.


Renal transplantation between HL-A identical donor-recipient pairs. Functional and morphological evaluation. 16 patients underwent renal transplantation from a sibling donor who was prospectively determined to be ABO compatible and HL-A identical with the recipient. Unidirectional mixed leukocyte reactions were performed; in each instance, lymphocyte stimulation in either direction was not observed. The plasma creatinine 10-68 months after transplantation in these 16 patients ranged between 0.9 and 1.9 mg/100 ml. The creatinine clearance ranged from 48 to 113 ml/min, and the blood urea nitrogen (BUN) ranged between 12 and 35 mg/100 ml. Urine protein excretion varied from 0.11 to 1.86 g/day. Six patients exhibited no detectable clinical episodes of acute rejection; they were treated with azathioprine alone and each of them demonstrated normal or near normal renal histology when biopsy specimens were obtained more than 6 months after transplantation. Nine patients experienced acute rejection episodes that required the use of steroid therapy. The severity of these rejection episodes was variable; they included a mild reduction in renal function with an immediate steroid-induced restoration of function and eventual discontinuance of steroid therapy to severe reduction in function requiring prolonged and moderate doses of steroids without return to normal renal function. Renal histological observations in this group ranged from mild to marked cellular and structural changes which fit the criteria of the rejection. One patient demonstrated a gradual loss of renal function with heavy proteinuria. Biopsy of this allograft demonstrated the recurrence of original disease, i.e., lobular glomerulonephritis. The marked variability in the clinical course and allograft morphology in these 16 patients could be explained by antigenic differences at non-HL-A loci. The presence of minor histocompatibility loci has been well documented in other mammalian species and they are most certainly present in man. The need for their identification and definition is stressed.


The arthritides: pathogenesis and radiologic appearance.


Effects of the carcinogen 2-acetylaminofluorene and its derivatives on bacteria and bacteriophages.


The pancreatic  -cell recognition of insulin secretagogues. V. Binding and stimulatory action of phlorizin.


Low resolution structure of yeast phosphoglycerate kinase.


Low resolution structure of yeast phosphoglycerate mutase.


Fate of abnormal proteins in E. coli accumulation in intracellular granules before catabolism.


Antibody of restricted heterogeneity secreted by spleen fragments.


Postmortem changes in the rat myocardium. A histologic and electron microscopic study.


Diffuse ultrastructural glomerular alterations in apparent 'focal' Schönlein-Henoch nephritis.


The parietal cell heteroantibody in human sera: prevalence in a normal population and relationship to parietal cell autoantibody.


The pathophysiology of gram-negative septicaemia.


Histological findings after experimental pulmonary autotransplantation.


Immediate changes in the pulmonary blood flow after various vascular anastomoses in lung autotransplantation.


Serum enzyme changes during modified cardiac rejection in dogs.


The complement sensitivity on PNH cells.


Thrombosis in paroxysmal nocturnal hemoglobinuria (PNH) with particular reference to progressive, diffuse hepatic venous thrombosis.


The laboratory substitutes of the red cell of paroxysmal nocturnal haemoglobinuria (PNH): PNH-like red cells.


Paroxysmal nocturnal haemoglobinuria: clinical manifestations, haematology, and nature of the disease.


Viewpoints on the management of paroxysmal nocturnal hemoglobinuria (PNH).


Granulocyte and platelet functions in paroxysmal nocturnal hemoglobinuria.


Diagnostic value of earpiece dye-dilution curve in aortic insufficiency.


Studies on craniopharyngiomas. I. Treatment: past and present.


Studies on craniopharyngiomas. II. Treatment by stereotaxis and radiosurgery.


Thermodilution measurement of cardiac output. A study in dogs comparing direct FICK with an automated thermodilution method (Fischer Cardiac Output Computer).


Intravenous antilymphocyte globulin in human cadaveric renal allotransplantation. Clinical and immunologic observations.


Properties of anti-dog lymphocytic globulin raised with two cell types and immunization schedules.


Scientific autographs. IX. Claude Bernard (1813-1878): public fame and private distress.


Use of the skin window for demonstration of lupus erythematosus cells.


Clinical, hematologic, and blood chemical changes in swine given endotoxin (Escherichia coli) during the immediate postpartum period.


Production of diarrhea in pigs in response to Escherichia coli enterotoxin.


Diagnosis of ovine vibriosis and enzootic abortion of ewes by immunofluorescence technique.


Estimation of leptospiral viability with agar pour plates.


The effect of a nebulized bronchodilator administered with or without intermittent positive pressure breathing on ventilatory function in children with cystic fibrosis and asthma.


Inhibition of the multiplication of Mycobacterium leprae by methimazole.


"Repair" of plasma membrane injury and DNA single strand breaks in  -irradiated Escherichia coli B-r and B S   .


Adenylic acid-specific limited digestion of tRNA with ribonuclease U 2  and the C-C-A terminal repair of resulting fragments.


Estimation of the double-helical content in various single-stranded nucleic acids by treatment with a single strand-specific nuclease.


Wendell G. Scott.

Wendell G. Scott.


Pharmacists, drugs, and health maintenance organizations.


Known and suspected teratogenic hazards in range plants.


Harvard School of Dentistry.

Harvard School of Dentistry.


DDS or DMD: Footnotes to dental history.


Effect of electrical stimulation of the hypothalamus on plasma free fatty acid concentration in cats. The effect of electrical stimulation of various hypothalamic regions on levels of plasma free fatty acids, glucose, triglycerides, and cholesterol was studied in fasted cats. Appreciable changes were observed in plasma free fatty acids and glucose but not in plasma triglycerides or cholesterol. These changes appeared to be dependent upon small differences in the placement of electrodes and could not be related to a distinct hypothalamic locus. The results indicate that there is a dissociation between hypothalamic neurons that may affect plasma glucose concentration and those that may affect the plasma free fatty acids. It is suggested that the hypothalamus of the cat contains neurons that may influence autonomic discharge to adipose tissue and thus affect the plasma free fatty acid level and other neurons that may influence autonomic discharge to the liver and thus affect glucose output into the circulation. The distribution of both types of neurons is not limited to a distinct region of the hypothalamus in cats.


New device for preparing thin slices of adipose tissue for metabolic studies in vitro. A new microtome is described which allows the rapid preparation of equal slices of well-defined thickness of fresh human tissue, especially adipose tissue. Presetting the microtome for a section thickness of 500 micro m, we found a variation of about 5% with human adipose tissue. Slices of human adipose tissue sliced by the microtome showed a higher sensitivity to insulin and a better reproducibility of results than slices prepared freehand.


Effects of selection on reproduction in avian species.


Genetic control of litter size in sheep.


Causal basis of chromosome abnormalities.


Immunogenetic aspects of maternal-fetal relations.


Ribosomal proteins. 38. Electron microscopy of ribosomal protein S4-16S RNA complexes of Escherichia coli.


Prophage induction and cell division in E. coli. I. Further characterization of the thermosensitive mutation tif-1 whose expression mimics the effect of UV irradiation.


Morphogenesis of the tail fiber of bacteriophage T 4 . V. In vitro complementation between the gene 36 product and the genes 37-38 directed component.


In vitro studies on the possible influence of Candida albicans on the growth of dermatophytes.


Survival, mutation and capacity to repair single-strand DNA breaks after gamma irradiation in different Exr -  strains of Escherichia coli.


Pulmonary edema. 1.

Pulmonary edema. 1.


Infantile hypersensitivity pneumonitis as a reaction to organic antigens.


The clinical use of diuretics. 1.

The clinical use of diuretics. 1.


Double-blind or single-blind?

Double-blind or single-blind?


Relationship of hypothalamic catecholamines and gonadotrophin levels in the chicken.


Effect of progesterone on the release of luteinizing hormone induced by a synthetic gonadotrophin-releasing factor in the ewe.


Effects of vaginal stimulation on hypothalamic multiple-unit activity and pituitary LH release in the rat.


Skin grafting.

Skin grafting.


Naturally-occurring antitumor agents.

Naturally-occurring antitumor agents.


Transferable multiple drug resistance of shigella strains isolated in Sweden.


The current status of transplantation immunology.


A study on the use of an active free-end palatal arch.


Clinical investigations of skeletal partial dentures with lingual splint (continuous clasp). II. Functional changes.


The case for historical research in nursing.


Deep hypothermia induced in the golden hamster by altering cerebral calcium levels.


Blood flow and volume redistribution with A-V shunt occlusion.


Effect of intrapulmonary water instillation on pulmonary lymph flow and composition.


Hodgkin's disease: cases with features of a community outbreak.


Bleeding times and platelet aggregation after analgesics in hemophilia.


Reversal of an inborn sphingolipidosis (Fabry's disease) by kidney transplantation.


Course on the social functions of prescription drugs. Seminar syllabus and bibliography.


Plague in San Francisco in 1900. The United States Marine Hospital Service Commission to study the existence of plague in San Francisco.


Allotransplantation for the treatment of inborn errors of metabolism.


One registry of patients with chronic renal failure.


Continuous positive-pressure ventilation.


The problem of the large enterocele.

The problem of the large enterocele.


The site of diazoacetyl inhibitor attachment to acid proteinase of Aspergillus awamori--an analog of penicillopepsin and pepsin.


Assymetric annealing of an RNA linked DNA molecule isolated during the initiation of bacteriophage T7 DNA replication.


Differential effects of aminoglycosides on cistron-specific initiation of protein synthesis.


Third annual David A. Karnofsky Memorial Lecture. The compleat clinical oncologist: a new approach to training.


The basis for progress in chemotherapy.

The basis for progress in chemotherapy.


A decade of combination chemotherapy of advanced Hodgkin's disease.


Non-Hodgkin's lymphoma: recent observations on natural history and intensive treatment.


Single and combination nonhormonal chemotherapy in breast cancer.


Plasma cell myeloma. An interpretive review.


Chemotherapy of malignant melanoma.

Chemotherapy of malignant melanoma.


Immunotherapy of cancer. Present status and future potential.


Renaissance in the microcirculation.

Renaissance in the microcirculation.


Dr. Solomon A. Berson.

Dr. Solomon A. Berson.


The ultrastructure of the human pancreatic islets. II. The islets of neonates.


Ultrastructural and biometric action of insulin on foetal rat pancreas.


A controlled study of a new analgesic (Z867) in post-operative urological patients.


Measurement of analgesic activity in man.


Clinical evaluation of the analgesic action of MZ-144 (Probon).


Cellular events during the induction of experimental thyroiditis in the rabbit. With a modification of the Jerne plaque technique to enumerate plaque-forming cells (PFC) to bovine and rabbit thyroglobulin, the cellular kinetics of the antibody response were followed during two 5-day series of injections of an aqueous preparation of bovine thyroglobulin. The results support the suggestion that thyroiditis in the rabbit is mediated by antibody. The peak PFC appear in the spleen at the end of the second series of injections and are considerably greater for bovine than for rabbit thyroglobulin. PFC also appear in the thyroid gland; however, the numbers of PFC for bovine and rabbit thyroglobulin were similar, and they did not reach a peak until 7 days after the peak PFC in the spleen. There was an excellent correlation between the appearance of PFC in the thyroid gland and the appearance of thyroid lesions. The disappearance of antibody to rabbit thyroglobulin from the serum also correlated with the appearance of lesions. Migration inhibition factor (MIF) activity was not produced at any time throughout the study when rabbit thyroglobulin was added to peritoneal exudates of immunized rabbits containing circulating antibody to rabbit thyroglobulin. MIF activity was observed when bovine thyroglobulin was added to similar cells in the later stages of the study after lesions were present.


Functional maturation of thymic lymphocyte populations in vitro. Mouse thymocytes were cultured for short periods of time either alone or with one of two supporting cell populations, splenic adherent cells or thymic epithelial cells. The thymus-derived (T) cell activity of thymocytes cultured on supporting cell populations increased dramatically during 2 days of culture, as assayed in the mixed lymphocyte interaction (MLI), response to phytomitogens, and helper cell activity in the in vitro antibody response. The level of activity attained was equal to that of spleen and lymph node lymphocytes and greater than that of steroid-resistant thymocytes. The cultured thymocytes had surface antigens characteristic of mature T lymphocytes with regard to theta and H-2. The appearance of functionally active lymphocytes in vitro depended upon cell division. Most of the active cultured cells arose from cells already undergoing maturation, i.e., from cells with reduced theta determinants and increased H-2 determinants. We therefore have generated a population of thymocytes indistinguishable from peripheral T lymphocytes using simple in vitro techniques. The extent to which the production of these active lymphocytes depends upon in vitro differentiation is discussed.


Induction of tolerance to sheep red blood cells. Adult mice injected with a sheep red blood cell hemolysate supernatant fraction were found to have a severely reduced responsiveness to subsequent immunization with sheep red blood cells. The induced unresponsiveness was found to be specific, as tested by reciprocal experiments involving horse and sheep erythrocyte preparations. The tolerogenic material did not appear to be immunogenic.


Prevalence of naturally occurring Toxoplasma gondii infections in cats from U.S. military installations in Japan.


Cultivation of feline Isospora rivolta in mammalian cells.


Immunological studies in chronic severe alveolar resorptive disease: a report of two young female patients.


Lowell Evan Noland 1896-1972. In memoriam.


Severe periodontal destruction following impression procedures.


Otto Jírovec, 1907-1972. In memoriam.

Otto Jírovec, 1907-1972. In memoriam.


Proteolytic activity of Cryptococcus neoformans against human plasma proteins.


Surgery for acquired valvular heart disease. 2.


Immunoglobulin on tumor cells and tumor-induced lymphocyte blastogenesis in human acute leukemia.


Centenary of Guy's Hospital Gazette--GMC inquiry: medicine then and now.


Hypersplenism in the uremic hemodialyzed patient. The effect of splenectomy--effect on transplantation and proposed mechanisms.


Spontaneous external rupture of an infected popliteal cyst in rheumatoid arthritis.


Binocular vision and cortical function.

Binocular vision and cortical function.


Trans-sphincteric exposure for low rectal anastomosis.


Transanal technique in low rectal anastomosis.


Cerebellar control of the vestibular pathways to oculomotor neurons.


Skin grafting using ethyl chloride.

Skin grafting using ethyl chloride.


Results in border moulding with a silicone functional impression material by different open- and closed-mouth techniques.


The effect of pre-impression mouth rinsing on the secretion rate of saliva.


Orthopedics in the Torah.

Orthopedics in the Torah.


Ilioinguinal lymph node dissection for melanoma.


Prevention and reversal of myocardial failure in endotoxin shock.


Evaluation of simple methods for assessing renal ischemic injury.


Cytodifferentiation.

Cytodifferentiation.


The evolution of the research on lens biology from 1924, the year Prof. dr. M.W. Woerdeman started experiments on the lens.


Right atrial pacing in coronary heart disease.


The early diagnosis of coronary heart disease, critical review.


The natural history of ischaemic heart disease.


Measurement of coronary blood flow by radiocardiography. Study of 116 cases.


The value of different angiographic procedures in coronary heart disease.


Significance of arterial obstructive lesions in early diagnosis of coronary heart disease.


Isolation and microbiologic differentiation of Klebsiella rhinoscleromatis and Klebsiella ozaenae in cases of chronic rhinitis.


Hospital airborne bacteria as estimated by the Andersen sampler versus the gravity settling culture plate.


A simple sedimentation chamber adaptable to the laboratory centrifuge.


Chopped-meat medium tube filler.

Chopped-meat medium tube filler.


In memoriam. James B. Hartney, M.D. 1921-1972.


In memoriam. Frederick A. Hemsath, M.D. 1894-1971.


In memoriam. Francis A. McKeon, Jr, M.D. 1924-1971.


In memoriam. Maurice L. Snell, M.D. 1919-1971.


Localized primary extranodal Hodgkin's disease.


Membranous glomerulonephritis. Evolution of clinical and pathologic features.


Olfactory status and response to clomiphene in male gonadotrophin deficiency.


Structure and movement of Entamoeba.

Structure and movement of Entamoeba.


Autoradiographic analysis of cell kinetics in human normal epidermis and basal cell carcinoma.


Inhibition of erythroleukemia in mice by induction of hemolytic anemia prior to infection with Rauscher leukemia virus.


Favorable remission induction rate with twice weekly doses of L-asparaginase.


Maintenance of normal, metaplastic, and dysplastic states of adult human bronchial mucosa in organ culture.


Toxicity of benzo( )pyrene and air pollution composite for adult human bronchial mucosa in organ culture.


Embolism: pulmonary and systemic.

Embolism: pulmonary and systemic.


Effects of malignant noncardiac tumors upon the cardiovascular system.


Pulmonary hypertension in pulmonary parenchymal disease.


Effect of temperature on the repair mechanism of radiation-induced damage in Escherichia coli.


Effect of trichloroacetate ion on the proteolytic activity of chymotrypsin, trypsin & pepsin.


Microvascular surgery: simplified instrumentation. Technical note.


Torulomas (cryptococcal granulomata) of the central nervous system.


Enterogenous cyst of the spinal cord with associated intestinal reduplication, vertebral anomalies, and a dorsal dermal sinus. Case report.


Vacuum extraction of hydrocephalic fetus.


History of the Liverpool School of Tropical Medicine.


Biochemistry at Liverpool 1902-1971.

Biochemistry at Liverpool 1902-1971.


History of the Liverpool teaching hospitals until 1907.


History of anaesthesia in Liverpool.

History of anaesthesia in Liverpool.


A note on the Liverpool Medical Institution.


Did a dental profession exist in ancient Egypt during the 3RD millennium B.C.?


Obituary: Dr. Louis Chauvois.

Obituary: Dr. Louis Chauvois.


Chemistry of thiamine degradation in food products and model systems: a review.


The physiology of drowning.

The physiology of drowning.


Amyloid joint disease.

Amyloid joint disease.


The problem of reduction in biology.

The problem of reduction in biology.


Infection of protoplasts from yeast with tobacco mosaic virus.


A possible role for histone in the synthesis of DNA.


Conjecture on the spread of infection in two dimensions disproved.


Lamellar transformation of lung mitochondria under conditions of stress.


Cadmium-induced testicular injury and alterations of androgen synthesis in brook trout.


Can "blocking" serum factors protect against autoimmunity?


Possible facilitated transport of oxygen across the placenta.


Influence of the embryo on the marsupial uterus.


Processes of synthesis in visual perception.


Release of brain dopamine as the probable mechanism for the hypothermic effect of D-amphetamine.


Unilateral inhibition of audiogenic seizures and Preyer reflexes.


Nanosecond laser photolysis of rhodopsin in solution.


Trends in the evolution of primate mastication.


Oxidative phosphorylation in adult Schistosoma mansoni.


Purity effects and distilled water taste.


Medical arch support by operation. Sustentaculum tali procedure.


Trephining your way.

Trephining your way.


Proceedings of the American Orthopaedic Foot Society, Inc. President's address.


The membrane actions of anesthetics and tranquilizers.


Mycoplasmosis: experimental mastitis. Immunoglobulin classes of mycoplasmal antibodies in milk and serum.


Platelet function tests and thrombosis.

Platelet function tests and thrombosis.


The role of platelets in arterial and venous thrombosis.


Methods for the preparation of washed platelet suspensions.


Aneurysms of the aorta.

Aneurysms of the aorta.


Diarrhoea in school-age South Indian children. 1. Preliminary observations on clinical features and aetiology. 2. Results of a therapeutic trial with rivanol-lactate and chlorostrep.


Anniversary of Professor V. Thurzo.

Anniversary of Professor V. Thurzo.


Nursing care study: malaria.

Nursing care study: malaria.


Study of cerebrospinal fluid in schizophrenics--a review of the literature.


A bibliography of papers from the Swiss literature, 1970-1971.


On the diamond fields around Kimberley.

On the diamond fields around Kimberley.


Anti-lymphocytic globulin in canine renal allotransplantation. I. Histologic and immunohistologic findings for two preparations given by subcutaneous and intravenous routes.


Anti-thymocytic globulin in canine renal allotransplantation. II. Immunosuppressive and toxic effect with subsutaneous and intravenous administration.


The effect of tranexamic acid (AMCA) on postoperative bleeding after conization.


Steroid-resistant nephrotic syndrome: recurrence after transplantation.


Mitochondrial antibody in primary biliary cirrhosis and other diseases.


Anaerobic pyogenic liver abscess.

Anaerobic pyogenic liver abscess.


VDRL in malaria.

VDRL in malaria.


Characterization of cholinesterases in various tissues of guinea pig with respect to their reactivation by obidoxime after inactivation by diisopropylfluorophosphate.


Candida infection.

Candida infection.


Significance of milk pH in newborn infants. Bottle-fed infants do not gain weight as rapidly as breast-fed babies during the first week of life. This weight lag can be corrected by the addition of a small amount of alkali (sodium bicarbonate or trometamol) to the feeds. The alkali corrects the acidity of cow's milk which now assumes some of the properties of human breast milk. It has a bacteriostatic effect on specific Escherichia coli in vitro, and in infants it produces a stool with a preponderance of lactobacilli over E. coli organisms. When alkali is removed from the milk there is a decrease in the weight of an infant and the stools contain excessive numbers of E. coli bacteria.A pH-corrected milk appears to be more physiological than unaltered cow's milk and may provide some protection against gastroenteritis in early life. Its bacteriostatic effect on specific E. coli may be of practical significance in feed preparations where terminal sterilization and refrigeration are not available. The study was conducted during the week after birth, and no conclusions are derived for older infants. The long-term effects of trometamol are unknown. No recommendation can be given for the addition of sodium bicarbonate to milks containing a higher content of sodium.


Viral infection and renal transplant rejection. The occurrence of an outbreak of influenza in a renal transplant unit is described. Five patients had a proved episode of infection, confirmed by a rise in the complement fixation titre to influenza virus A, and this coincided in three of the patients with episodes of acute rejection. It seems likely that the virus infection was responsible for the rejection, possibly through a stimulating effect of the virus on the host's immune response.


Specific IgM antibody in serum of patients with herpes zoster infections. Specific IgM varicella-zoster antibody was detected in "convalescent" sera from 20 out of 40 patients (50%) with herpes zoster infections. Since these were not primary infections with varicella-zoster virus, it seems that detection of IgM antibody specific for a particular virus may not differentiate a primary infection from secondary infections with that virus.


Outpatient surgery in children.

Outpatient surgery in children.


Sjögren's syndrome.

Sjögren's syndrome. Sjögren's syndrome is a common accompaniment of rheumatoid arthritis and other connective-tissue diseases as well as several diseases thought to have an immunological basis. Despite the wide spectrum of clinical features and serological abnormalities the condition is, if anything, underdiagnosed. A clinical subgroup of patients with Sjögren's syndrome can be distinguished by their pronounced hypergammaglobulinaemia, widespread and prominent immunological abnormalities, severe salivary gland swelling, and mild or absent arthritis. It is in this group that lymphoma development has been a complication.


Surface antigens on Schistosoma mansoni. I. Demonstration of host antigens on schistosomula and adult worms using the mixed antiglobulin test.


Fibrillar anti-cellular antibody associated with mumps and measles infection.


In vitro nutritional requirements of Nippostrongylus brasiliensis. I. Effects of sterols, sterol derivatives and heme compounds on the free-living stages.


The pigment formed during haemoglobin digestion by malarial and schistosomal parasites.


50th birthday of V. Zalabák.

50th birthday of V. Zalabák.


Turnover of murein in a diaminopimelic acid dependent mutant of Escherichia coli.


Transport of histone into Escherichia coli.


Joined suppression of rough phenotype and of red colour of ade2-1 mutants in Saccharomyces cerevisiae.


Automated sampling during continuous cultivation.


Dr. Kamila Vágnerová, CSc. (1927-1972).

Dr. Kamila Vágnerová, CSc. (1927-1972).


Citation for William Richard Hendee.

Citation for William Richard Hendee.


Evaluation of search time for two computerized information retrieval systems at the University of Georgia.


Clive Maine McCay (1898-1967)--a biographical sketch.


The use of corticosteroids in celiac crisis.


The glomerulopathy of congenital syphilis--an immune deposit disease.


Countercurrent immunoelectrophoresis in the diagnosis of systemic diseases caused by Hemophilus infleunzae type b.


Infections acquired in a pediatric hospital.


Positive end-expiratory pressure. Physiology, indications, and contraindications.


Spontaneous breathing with continuous positive airway pressure after open intracardiac operations in infants.


Functions of canine lung allografts.

Functions of canine lung allografts.


Serial changes in pulmonary blood flow occurring during acute rejection of a lung allograft.


Mitral valve replacement with autologous fascia lata. Results of morphologic examinations.


Cross hybridization and rate of chain elongation of the two classes of DNA intermediates.


Genetic control over the initiation of the synthesis of the short deoxynucleotide chains in E. coli.


Stringent control in Escherichia coli.

Stringent control in Escherichia coli.


The development of body image in the child.


Mutant E. coli strain with temperature sensitive peptidyl-transfer RNA hydrolase.


Care of a baby with a bone marrow transplant.


Fertility in the domestic hen following the surgical removal of the utero-vaginal junction.


A re-evaluation of the ovulatory cycle of the domestic hen (Gallus domesticus).


A strain of Escherichia coli with minimum postirradiation degradation properties.


Obituaries. Wright H. Langham. (1911-1972).


The radiosensitivity of cultured mammalian cells exposed to single high intensity pulses of electrons in various concentrations of oxygen.


Dr. Vilmos Várterész. (1917-1972).

Dr. Vilmos Várterész. (1917-1972).


Maciej Marcia Tabaczyński.

Maciej Marcia Tabaczyński.


Mutations affecting superinfection immunity in Escherichia coli.


Transformation of col V and col I factors.


The genetic control of the arginine pathways in Aspergillus nidulans mutants blocked in arginine biosynthesis.


On the phosphorylation of Escherichia coli ribosomes. I. An in vivo labeling of ribosomes.


Pectinolytic activity of rumen streptococci.


Polifungin, a new antifungal antibiotic. 3. Biochromatographic studies on polifungin complex produced by Streptomyces noursei var. polifungini and its mutants.


Comparison of histamine and caerulein stimulation of gastric secretion in the rat under normal oxygen conditions and in hypoxia.


Carl V. Moore, M.D. 1908-1972.

Carl V. Moore, M.D. 1908-1972.


The pineal gland--a neuroendocrine transducer.


Severe hypertension and elevated plasma renin activity following transplantation of "hepatorenal donor" kidneys into anephric recipients.


Acute renal carbuncle. The roentgenographic clarification of a medical enigma.


The American Journal of Roentgenology Radium Therapy and Nuclear Medicine.


Roentgen appearance of bone and soft tissue changes in chronic renal disease.


Late results of successful replantation of upper and lower extremities.


Wound complications in recipients of renal transplants.


Problems of verrucose squamous carcinoma.


DDS: mechanisms of action and structure-activity relationships.


Studies on deoxyribonucleic acid-dependent ribonucleic acid polymerase from Escherichia coli. Variations of the enzyme activity during growth. 1. RNA polymerase activity of Escherichia coli extracts prepared from cells in exponential and stationary phases of growth, when measured in the presence and absence of external template, showed significant qualitative differences. 2. In both extracts, polymerase activity was higher when assayed with external template, suggesting the presence of a pool of enzyme not bound to cellular DNA. 3. In the crude extract, the fraction of enzyme bound to cellular DNA is higher during the exponential phase of growth. 4. A method is described for the purification of enzyme molecules not tightly bound to cellular DNA from exponential- and stationary-phase cultures. 5. Purified enzyme preparations showed differences in template requirement and subunit composition. 6. On phosphocellulose chromatography of stationary-phase enzyme, a major portion of polymerase activity eluted from the column with 0.25m-KCl. In the case of exponential-phase enzyme, polymerase activity eluted from a phosphocellulose column mainly with 0.35m-KCl. 7. Enzyme assays done with excess of bacteriophage T(4) DNA showed a strong inhibition of stationary-phase enzyme by this template. The exponential-phase enzyme was only slightly inhibited by excess of bacteriophage T(4) DNA.


Effects of organic mercurials on mammalian pancreatic  -cells. Insulin release, glucose transport, glucose oxidation, membrane permeability and ultrastructure. The effects of p-chloromercuribenzoic acid and chloromercuribenzene-p-sulphonic acid on pancreatic islets were studied in vitro. Obese-hyperglycaemic mice were used as the source of microdissected islets containing more than 90% beta-cells. p-Chloromercuribenzoic acid and chloromercuribenzene-p-sulphonic acid stimulated insulin release at concentrations of 0.01mm or above. This stimulation was significantly inhibited by the omission of Ca(2+) or the addition of adrenaline, diazoxide or 2,4-dinitrophenol. p-Chloromercuribenzoic acid or chloromercuribenzene-p-sulphonic acid did not interfere with the insulin-releasing ability of glucose. Micro-perifusion experiments revealed that the release of insulin in response to organic mercurial occurred almost instantaneously, was reversible, and was biphasic. The two mercurials inhibited glucose transport as well as glucose oxidation, and increased the mannitol and sucrose spaces of isolated islets. Compared with the effects on insulin release, those on glucose transport and membrane permeability were characterized by a longer latency and/or required higher concentrations of organic mercurial. Apart from a seemingly higher proportion of beta-cells exhibiting certain degenerative features, in islets exposed to 0.1mm-chloromercuribenzene-p-sulphonic acid for 60min, no significant differences with respect to beta-cell fine structure were noted between non-incubated islets and islets incubated with chloromercuribenzene-p-sulphonic acid or glucose or both. It is suggested that insulin release may be regulated by relatively superficial thiol groups in the beta-cell plasma membrane.


Tragic dilemma.

Tragic dilemma.


Malaria in immigrant Pakistanis.

Malaria in immigrant Pakistanis.


Effects of four commonly-used tranquillizers on low-speed driving performance tests. A double-blind controlled comparison of four commonly-used tranquillizing drugs (haloperidol, amylobarbitone sodium, chlordiazepoxide, and trifluoperazine) against placebo was made in their effects on the performance of volunteers during three low speed vehicle-handling tests. The drugs (with the exception of haloperidol) significantly altered driving behaviour though they did not seem to interact significantly with alcohol. There is, therefore, a strong possibility that such drugs will similarly alter driving performance in patients taking them for therapeutic purposes. Since, as these experiments also show, those affected may be subjectively unaware of it, and routine clinical screening is not sensitive enough to detect them, physicians should warn patients of the probability that their driving performance will be affected by such drugs, particularly during the first few days that they are taken.


Major central nervous system malformations notified in Northern Ireland 1964-1968.


Treatment of cerebral irritation in the newborn: double-blind trial with chloral hydrate and diazepam.


Laterality and dominance.

Laterality and dominance.


Conservative treatment of hydrocephalus.


Studies on the structuredness of cytoplasm and rates of enzymatic hydrolysis in growing yeast cells. II. Changes induced by ultra-violet light.


Host-cell reactivation of phiX174 RF-DNA damaged by  -ray-induced phenylalanine and water-radicals.


Microbiological hydroxylation of steroids. V. The pattern of hydroxylation of dioxygenated 5 -androstanes with cultures of the fungus Calonectria decora.


4-Chloramphetamines. Chance and trend in the development of new antidepressants.


A comparison of fluspirilene and trifluoperazine in the treatment of acute schizophrenic psychosis.


A controlled evaluation of propranolol in chronic alcoholic patients presenting the symptomatology of anxiety and tension.


Roster of members. Indiana State Medical Association and Woman's Auxiliary.


Clinical trial of benzoctamine versus chlordiazepoxide in anxiety neurosis.


The beloved physician.

The beloved physician.


The legal and ethical considerations of clinical research.


Epigenetic transformation by RNA from human neoplastic cells.


Effect of diet on levels of amino acids in plasma and tissues.


Genetic control of susceptibility to the avian leukosis complex. 2. Marek's disease.


Poultry feed radicidation. I. Microbiological aspects of poultry feed irradiation.


Dietary factors influencing amino acid degradation.


Toxigenic fungi from poultry feed and litter.


Amino acid and mineral interrelationships.


Development and application of amino acid diets.


Structure and function of immunoglobulin A.


Double-blind trial with chloramphenicol and the combination trimethoprim-sulfamethoxazole in typhoid.


Etiology and pathogenesis of congenital cleft lip and cleft palate, an NIDR state of the art report.


The influence of the fetal genome on trypan blue-induced cardiovascular defects.


Host repopulation of endothelium in human kidney transplants.


Cross reactions of HL-A antibodies. I. Characterization by absorption and elution.


The proliferative potential of frozen stored human marrow cells.


Ionic and histological changes in the kidney after perfusion and storage for transplantation: use of high Na- versus high K-containing solutions.


Various effects of alloantibody-containing globulins on the time of retention of skin allografts in the mouse.


Effects of temperature on kidneys preserved by hypothermic perfusion.


Treatment of arterial lesions in cardiac allografts.


The absence of early rejection crises in human renal allografts as predicted by capillary agglutinating antibodies.


Histoincompatibility between the B10.D2-n and B10.D2-0 strains of mice.


Mechanism for the prolonged survival of glutaraldehyde-treated skin allografts.


Phagocytosis of mutants of Salmonella typhimurium by rabbit polymorphonuclear cells.


Rabbit anti rat lymphocyte serum: immunosuppression mediated by IgM antibodies.


Evaluation of disinfectant inactivators.


Transferable and non-transferable drug resistance in enteric bacteria isolated from urinary specimens in Northern Sweden.


Haemophilus in the upper respiratory tract of children. A bacteriological, serological and clinical investigation.


Direct and indirect immunofluorescence of unfixed and fixed Mycoplasma colonies.


Maternal age and spina bifida: longitudinal versus cross-sectional analysis.


Enteric transmission of parvoviruses: pathogenesis of rat virus infection in adult rats.


Direct cementation of orthodontic attachments.


Starch granulomas.

Starch granulomas.


Direct immunofluorescence of bullous systemic lupus erythematosus.


Alterations of cell surfaces as a pathogenetic factor in psoriasis. Possible loss of contact inhibition of growth.


Electron microscopic studies on penetration and development of Trypanosoma cruzi in HeLa cells.


The intermediacy of 3-oxo steroids in the conversion of cholest-5-en-3 -ol into 5 -cholestan-3 -ol by the starfish Asterias rubens and Porania pulvillus.


The control of ribonucleic acid synthesis in bacteria. Polymerization rates for ribonucleic acids in amino acid-starved relaxed and stringent auxotrophs of Escherichia coli. Polymerization rates of newly formed chains of various RNA fractions were measured in Escherichia coli CP78 (RC(str)) and CP79 (RC(rel)) multiple amino acid auxotrophs, deprived of four amino acids essential for growth. Immediately after the onset of severe amino acid deprivation, in RC(str) strains the rate of labelling of RNA by exogenous nucleotide bases was greatly diminished. At first, the initiation of new RNA chains declined faster than the rate of polymerization in RC(str) organisms, but as starvation proceeded the rate of polymerization was eventually lowered to about 10% of that found during normal growth. In strain CP79 (RC(rel)), on the other hand, chain-polymerization rates were unaffected by amino acid withdrawal. Artificial depletion of the intracellular purine nucleotide pools in RC(str) or RC(rel) strains by trimethoprim, before the onset of amino acid deprivation, showed that in the RC(str), but not the RC(rel) strain, amino acid withdrawal gave rise to an inability of the cells to utilize exogenously supplied purine or pyrimidine bases for RNA synthesis. During a prolonged starvation, the observed 100-fold decrease in the total rate of incorporation of exogenous nucleotide bases into the RNA of RC(str) organisms was ascribed to a combination of a tenfold decrease in the overall rate of RNA chain polymerization, at least a fivefold decrease in the ability of the cells to utilize exogenous bases and a preferential inhibition of initiation of stable RNA chains. None of these changes occurred in the corresponding RC(rel) strain.


The control of ribonucleic acid synthesis in bacteria. The synthesis and stability of ribonucleic acids in relaxed and stringent amino acid auxotrophs of Escherichia coli. The biosynthesis and stability of various RNA fractions was studied in RC(str) and RC(rel) multiple amino acid auxotrophs of Escherichia coli. In conditions of amino acid deprivation, RC(str) mutants were labelled with exogenous nucleotide bases at less than 1% of the rate found in cultures growing normally in supplemented media. Studies by DNA-RNA hybridization and by other methods showed that, during a period of amino acid withdrawal, not more than 60-70% of the labelled RNA formed in RC(str) mutants had the characteristics of mRNA. Evidence was obtained for some degradation of newly formed 16S and 23S rRNA species to heterogeneous material of lower molecular weight. This led to overestimations of the mRNA content of rapidly labelled RNA from such methods as simple examination of sucrose-density-gradient profiles. In RC(rel) strains the absolute and relative rates of synthesis of the various RNA fractions were not greatly affected. However, the stability of about half of the mRNA fraction was increased in RC(rel) strains during amino acid starvation, giving kinetics of mRNA labelling and turnover that were identical with those found in either RC(str) or RC(rel) strains inhibited by high concentrations of chloramphenicol. Coincidence hybridization techniques showed that the mRNA content of amino acid-starved RC(str) auxotrophs was unchanged from that found in normally growing cells. In contrast, RC(rel) strains deprived of amino acids increased their mRNA content about threefold. In such cultures the mRNA content of accumulating newly formed RNA was a constant 16% by wt.


Preparation and characterization of the lateral and basal plasma membranes of the rat intestinal epithelial cell. A technique is described for the isolation of a plasma-membrane fraction from the rat intestinal epithelial cell which is distinct from the microvillus membrane of that cell. The isolated fraction contains only about 0.2% of the sucrase activity in the original homogenate and negligible quantities of nuclear and mitochondrial membrane markers. It contains 12% of the total Na(+),K(+)-dependent adenosine triphosphatase and 7% of the alkaline phosphatase, with significant increments in specific activity of these enzymes. Multiple membrane preparations were highly reproducible with respect to the specific activities of the markers studied. The small intestine of one rat yields material containing about 1.3mg of protein. In addition an assay is described suitable for determining 5'-nucleotidase in the small intestine.


Nucleotide sequences of two fragments from the coat-protein cistron of bacteriophage R17 ribonucleic acid. Bacteriophage R17 RNA was labelled with (32)P and was subjected to partial digestion with ribonuclease T(1). The products were fractionated by ionophoresis on polyacrylamide gel. Two fragments were purified and their nucleotide sequences determined by methods involving complete and further partial digestion with ribonucleases A and T(1). Fragment 20 had a sequence that coded for the amino acids in positions 32-53 of the coat protein of the bacteriophage. Fragment 20X, on further purification in 7m-urea, gave rise to two smaller nucleotides whose sequences coded for the amino acids in positions 56-66 and 67-76 of the coat protein. The sequence of the two fragments was such that they could be written in the form of loops stabilized by base-pairing.


The social and emotional adjustment of deaf and partially deaf children.


Cell coupling in developing systems: the heart-cell paradigm.


Studies on the development of immunity: the response to sheep red blood cells.


Regulation of sporulation in yeast.

Regulation of sporulation in yeast.


Genetic map of the lactose repressor gene (i) of Escherichia coli. 39 tonB i(-) deletions were used to map 57 independently isolated i gene mutants. Methods selective for i(+) recombinants from various types of i mutants are described. i(+) recombination frequencies of 6 x 10(-6) to 6 x 10(-7) can be detected in the different selective systems. Twenty i(s) mutations and 12 i(-d) mutations map in distinct but different regions of the gene. i(-) (sus) mutations are scattered over the gene.


Bacterial conjugation: an analysis of mixed recombinant clones. A fraction of recombinant colonies resulting from conjugation is heterogenetic for unselected markers. Constitutivity for alkaline phosphatase synthesis (phoR) is studied as the unselected marker. The frequency of phoR heterogeneity depends on the genetic distance between phoR and the selected marker. Various models are considered which explain the formation of heterogenetic colonies (mixed clones), and experiments are described which test these models. It is concluded that the Hfr fragment can replicate and participate more than once in recombination thus yielding heterogenetic colonies.


Propranolol prophylaxis of migraine.

Propranolol prophylaxis of migraine.


Congressional response to a recognized need--drugs under Medicare.


Toxoplasma serologic survey in man and domestic animals in Egypt.


Effect of paramagnetic ions upon formation of spore photoproduct in DNA irradiated with UV at -196 degrees C.


The therapy of osteogenic sarcoma: current status and thoughts for the future.


Lafayette Houghton Bunnell, M.D. of Homer, Minnesota. Discoverer of the Yosemite.


The effect of cis-dichlorodiammineplatinum(II) on Escherichia coli B. The role of fil, exr and hcr markers.


Chloramphenicol effects on DNA replication in UV-damaged bacteria.


Effect of the recC gene in Escherichia coli on frequencies of ultraviolet-induced mutants.


The mutagenicity of benzimidazole and benzimidazole derivatives. II. Incorporation of benzimidazole into the nucleic acids of Escherichia coli.


Fat embolism.

Fat embolism.


John Richard Hertslet Fulton.

John Richard Hertslet Fulton.


John Dreadon.

John Dreadon.


The management of cervical incompetence. UCLA experience with cerclage procedures.


The induction of labor at term. Comparisons between prostaglandin F 2   and oxytocin infusions.


Contribution to the technic of tubal ligation.


Bacteriuria in pregnancy. A heterogeneous entity.


Retarded growth of E coli in amniotic fluid.


Significance of routine post-mortem bacteriological cultures.


Fatal Streptococcus pyogenes septicemia associated with an intrauterine device.


Modifications of collagen and non fibrillar-proteins and cathepsin activity in the carrageenin granuloma.


Choice of gender on the DAP and measures of sex-role identification.


Ophthalmological effects of nortriptyline--relationship to plasma level.


Potentiation between pentagastrin and histamine in stimulation of secretion from totally isolated ex vivo canine stomach.


Molecular properties of choline acetyltransferase and their importance for the compartmentation of acetylcholine synthesis.


Localization of transmitter candidates in the hippocampal region.


Cystitis emphysematosa.

Cystitis emphysematosa.


Regional lung volumes with positive pressure inflation in erect humans.


Vocational adjustment, interests, work values, and career plans of patients awaiting renal transplantation.


Vascular complications in 155 consecutive kidney transplantations.


Silent iliac compression syndrome as a cause of renal vein thrombosis after transplantation.


Brain scanning in children.

Brain scanning in children.


Use of nuclear imaging in the evaluation of pediatric cardiac disease.


Regional medical program of Texas. Kidney program.


Fibrin degradation products in renal parenchymal disease states and renal transplant patients.


Erythrocyte deformation as a sign of renal homograft rejection.


Serial determination of blast cell count during allograft rejection in rats.


Failure to detect cytotoxic lymphoid cells or humoral blocking factors in mouse radiation chimaeras.


Hypothermic pulsatile perfusion of dog kidneys. Factors limiting successful preservation time.


H-2 antigens on mouse spermatozoa.

H-2 antigens on mouse spermatozoa.


Evidence of immune response to orthotopic tooth allografts in Syrian hamsters.


Technical notes on lung transplantation with donor survival in inbred beagles.


The renal xenograft rejection. Effect of hyperimmune F(ab') 2  and succinylated IgG.


HL-A antigens on glomeruli. Demonstration by an immunofluorescent technique.


Differential effect of adult thymectomy in mice treated with antilymphocyte or antithymocyte serum.


The response of the dental profession to change in the organization of health care--a commentary.


5-Fluorocytosine therapy.

5-Fluorocytosine therapy.


Mark Twain, social critic, and his image of the doctor.


Treatment of lupus nephritis with prednisone and combined prednisone and azathioprine.


Acute respiratory distress syndrome in pancreatitis.


Mitral valvular disease. A clinicopathologic survey of the conditions causing the mitral valve to function abnormally.


Wheezing in childhood.

Wheezing in childhood.


An attempt to culture Mycobacterium leprae in cell-free, semisynthetic, soft agar media. This paper describes an attempt to culture M. leprae from leprous nodules, using cell-free, semisynthetic, soft agar media, and the effects of varying the incubation temperature and the composition of the basic medium, as well as of pretreating the bacterial suspensions with alkali. The identification test carried out in leprosy patients by intradermal injection of bacillary suspensions prepared from the primary cultures and third subcultures suggested that the bacterial masses that had formed in the suspensions were composed of M. leprae.


A comparison of 5-fluorouracil administered by slow infusion and rapid injection.


Hepatitis with Australia antigenemia following renal transplantation. Over a seven-year period 18 of 125 patients who underwent renal transplantation developed hepatitis. Acute hepatic necrosis occurred in two, chronic aggressive hepatitis progressing to posthepatitic cirrhosis in eight, chronic persistent hepatitis in five, acute hepatitis with recovery in two and cholestatic hepatitis in one. Hepatic failure was the cause of death in four and a major contributing factor in three. Fifteen of the 18 were of blood Group A. After renal transplantation Australia antigen (Au) was present in the blood of 12 of the 15 patients with hepatitis who were tested and in one of 38 patients without clinical evidence of liver disease. Once present, Au persisted in all patients but one. Particles measuring 210 to 250 A, characteristic of Au, were seen in liver cells by electronmicroscopy in nine of the 10 patients examined who had hepatitis with Australia antigenemia, but they were not seen in the two patients studied with Au-negative hepatitis.


In memory of Nguyen Phuc Buu-Hoi. 1915-1972.


Stimulation of FSH release in vivo by prolonged infusion of synthetic LH-RH.


Enhancement of the response of T-1 human kidney cells to thyroidal hormones by diminishing serum content of the growth medium.


Requirement for adherent cells in the primary and secondary immune response in vitro.


Preferential induction of cell-mediated immunity by chemically modified sheep erythrocytes.


Induction of immunity and tolerance in vitro by hapten protein conjugates. II. Carrier independence of the response to dinitrophenylated polymerized flagellin.


Detection and measurement of single-strand breaks in nuclear DNA in fixed lens sections.


Cell cycle analysis by culture fractionation.


Whole mount electron microscopy of metaphase. I. Chromosomes and microtubules from mouse oocytes.


Isolation and culture of homogenous populations of primordial germ cells in chick.


Differential immunofluorescent labeling of chromosomes with antisera specific for single strand DNA.


A TV-based technique for optical analysis of chromosome regions.


Synchronization of haploid yeast cell cycles, a prelude to conjugation.


An evaluation of the oscillatory repression model of periodic enzyme synthesis in yeast.


Effects of cytochalasin B on cultivated human diploid fibroblasts and its use for the isolation of tetraploid clones.


Specific detection of kinetoplast DNA in cytological preparations of trypanosomes by hybridization with complementary RNA.


Differentiation, contact inhibition and intercellular communication in retinal pigment cells.


Analysis of the specificity of allocluster-forming lymphocytes in allografted mice.


Reversible arrest of haploid yeast cells in the initiation of DNA synthesis by a diffusible sex factor.


Spontaneous autoimmune disease in mouse strains other than the NZB strain.


American College of Dentists Foundation.


Citation for honorary fellowship to Lauriston S. Taylor.


Citation for the William John Gies Award to Harry W. Archer.


Citation for the Award of Merit to John W. Kumpula.


Citation for the William John Gies Award to Maynard K. Hine.


Willard C. Fleming 1899-1972.

Willard C. Fleming 1899-1972.


Dental licensure and the development of dentistry in the United States.


The president of the college.

The president of the college.


A comparison of cutting efficiency of air-driven fissure burs.


Possible electromagnetic interference with cardiac pacemakers from dental induction casting machines and electrosurgical devices. Council on Dental Materials and Devices.


Effect of 30S ribosomal subunits on polyphenylalanine synthesis in Escherichia coli.


Preparation and some properties of three amino acid polymerization factors from silk gland of silkworm.


Estimation of polypeptide chain molecular weights of muscle proteins by gel filtration in 5M guanidine hydrochloride.


Partial digestion of 50S ribosomes of E. coli. II. Digestion of the CsCl treated ribosomes with RNase T 1 .


Studies on the primary structure of sulfate binding protein from Salmonella typhimurium. I. Tryptic digestion.


Studies on the primary structure of the sulfate binding protein from Salmonella typhimurium. II. Thermolysin digestion.


Studies on the primary structure of the sulfate binding protein from Salmonella typhimurium. 3. Digestions with pepsin and dilute hydrochloric acid.


Sensitivity of Escherichia coli to viral nucleic acid. V. Competence of calcium-treated cells.


Transverse furrows of the nails associated with Plasmodium vivax malaria.


Leprosy over the last 25 years.

Leprosy over the last 25 years.


Postoperative jaundice.

Postoperative jaundice.


Banding patterns in human chromosomes: production by proteolytic enzymes.


Pulmonary edema. 2.

Pulmonary edema. 2.


Fluoride in dental plaque.

Fluoride in dental plaque.


Hepatitis-associated antigen.

Hepatitis-associated antigen.


Location of the gene for Theta antigen in the mouse.


The J. Earle Hiltz Memorial Lecture.

The J. Earle Hiltz Memorial Lecture.


Recollections of 40 years of hospitalization of children.


Acceptance of the Howland award.

Acceptance of the Howland award.


Unusual segmental cerebrovascular changes.


Photographic anisotropic subtraction and edge enhancement.


Early evaluation of cadaver renal allotransplant by means of radionuclide imaging.


In memoriam. Bernard W. Robinson, M.D.

In memoriam. Bernard W. Robinson, M.D.


Comparative multiclinical studies of iodamide and diatrizoate in excretory urography.


The effect of vinblastine and demecolcine on circulating megakaryocytes in adult rats.


Surgery of osteoarthritis.

Surgery of osteoarthritis.


Diabetes mellitus and gout.

Diabetes mellitus and gout.


The acute and prolonged effects of curde hypothalamic extract on the anterior pituitary glands cells. An electron microscopic study. I. Somatotrophs and mammotrophs.


Light, fluorescence, and electron microscopic investigations of the peripheral autonomic nervous system in frog pancreas with particular reference to monoamines and acetylcholinesterase activity in the islets of Langerhans.


Sympathetic ganglia in culture. I. Neurons.


Associate Professor Leon Bozko.

Associate Professor Leon Bozko.


Utilization of aromatic compounds by benthic microorganisms of an eutrophic lake.


Experimental study on the mechanisms of delayed hypersensitivity reaction, with special reference to emigration and function of mononuclear cells.


The behaviour of lymphogeneously given antigen in the lymph node--the localization of given antigen and newly formed antibody.


Ecological patterns of udder infection.

Ecological patterns of udder infection.


The natural history of obesity.

The natural history of obesity.


Hydrogen peroxide bleach technique in the diagnosis of malignant melanoma.


Disturbances in the regulation of food intake. Obesity: anatomic and physioligic-biochemical observations.


The phenomenon of regeneration in fungal protoplasts.


Epidemiologic considerations.

Epidemiologic considerations.


Eating behavior and obesity in men and animals.


Psychiatric observations on obesity.

Psychiatric observations on obesity.


Experimental observations on anorexia.

Experimental observations on anorexia.


Anorexia nervosa and other anorectic states in man. General clinical considerations.


Squashing under collodion membrane for permanent cytologic preparations.


A simple device for red cell saponization.


Regulation of food intake. Physiologic-biochemical aspects (peripheral regulatory factors).


Psychiatric observations on anorexia.

Psychiatric observations on anorexia.


Mental health of political candidates: notes on Abraham Lincoln.


Depot phenothiazine treatment in acute psychosis: a sequential comparative clinical study.


Subjects' recent life changes and their near-future illness reports.


The validity and usefulness of the end-tidal pCO 2  during anaesthesia.


Haemodynamic alterations produced by changes in posture with special reference to the usefulness of isotope  113m In radiography.


The fine points of acupuncture.

The fine points of acupuncture.


Relation of nutrition to physical and mental development.


The effect of infection on nutritional status.


Dietary treatment of inborn errors of amino acid and carbohydrate metabolism.


Supplementary feeding of infants and young children.


Significance of the salt level in food for infants and children.


Enrichment and fortification of foods for infants and children.


Nutritional value of commercially produced foods for infants.


Technology of protein synthesis and protein-rich foods.


Recommended intakes of vitamins for normal growth and development.


Mineral and trace element requirements for normal growth and development.


Protein and energy requirements of children.


Loss of vitamins in industrially handled foods and its prevention.


Effects of oxathiin systemic fungicides on various biological systems.


Treatment of cerebral aneurysms by stereotaxic ferromagnetic silicone thrombosis. Case report.


Mechanisms of bacterial resistance to antibiotics.


The challenge of life. Biomedical progress and human values. Roche anniversary symposium. Welcoming address.


Einar Lundsgaard, 1889-1968.

Einar Lundsgaard, 1889-1968.


The distribution of a blastokinin-like uterine protein studied by immune fluorescence.


Suggestions for contracts for sisters' services.


Medicare reimbursement pitfalls.

Medicare reimbursement pitfalls.


Iatrogenic neurological disorders.

Iatrogenic neurological disorders.


Incidence of spina bifida occulta in Andhra area in India.


The effect of burning on leucocyte ascorbic acid and the ascorbic acid content of burned skin.


Guide to state associations of licensed practical/vocational nurses and State Boards of Nursing for LP/VNs.


The identification of marijuana.

The identification of marijuana.


Binding of bacteriophage P22 tail parts to cells. Purified base-plate parts of bacteriophage P22 can bind to the host cell, Salmonella typhimurium. Although the reaction is reversible, a stable equilibrium is not formed between bound and unbound base-plate parts. This is because the binding sites on the cell, presumably the O antigens, are destroyed. The destruction of binding sites does not kill the cells, and, in fact, the binding sites are soon regenerated. The site-destroying activity reacts with P22 heads to make active phage and with antiserum made against purified phage. Therefore site-destroying activity is a characteristic of the base-plate parts and not some contaminant of the preparation.


Host- and phage-mediated repair of radiation damage in bacteriophage T4. T4+ exhibits increased ultraviolet sensitivity on derivatives of Escherichia coli K12 or B lacking deoxyribonucleic acid (DNA) polymerase I. However, the sensitivity of T4v is not affected by the absence of host DNA polymerase. T4x and T4y also show increased sensitivity on DNA polymerase-deficient strains, but to a lesser extent than observed with wild-type T4. When T4x or T4y, but not T4+, are plated on a double mutant lacking both DNA polymerase and the uvrA gene product, a partial suppression of the polymerase effect is observed. Host ligase appears to be able to suppress to some extent the T4y phenotype but has no effect on wild-type T4 or other T4 mutants. T4xv incubated in E. coli B or B(s-1) in the presence of chloramphenicol (50 mug/ml) shows increased resistance over directly plated irradiated phage. Increased survival under the same conditions was not observed with T4+ or other T4 mutants. The repair of X-ray-damaged T4 was investigated by examining survival curves of T4+, T4x, T4y, T4ts43, and T4ts30. The repair processes were further defined by observing the effects of plating irradiated phage on various hosts including strains lacking DNA polymerase I or polynucleotide ligase. Two classes of effects were observed. Firstly, the x and y gene products seem to be involved in a repair system utilizing host ligase. Secondly, in the absence of host DNA polymerase, phage sensitivity is increased in an unknown manner which is enhanced by the presence of host uvrA gene product.


Asymmetric transcription of bacteriophage Mu-1. The deoxyribonucleic acid (DNA) of bacteriophage Mu-1 can be separated into its complementary strands by poly(U,G) binding and equilibrium centrifugation. DNA-ribonucleic acid (RNA) hybridizations in liquid show that more than 98% of "early" phage-specific RNA and over 96% of "late" messenger species bind to the heavy [poly(U,G)-binding] strand of Mu-1 DNA. A small (1.45%) but significant amount of late RNA binds to the light strand. The significance of this RNA fraction is discussed in connection with the peculiar structure of denatured and reannealed Mu-1 DNA.


Polypeptide synthesis by extracts from Escherichia coli treated with T2 ghosts. Infection of Escherichia coli B in inorganic salts-glycerol with a multiplicity of deoxyribonucleic acid-less T2 "ghosts" just sufficient to block all protein synthesis results in both viable and killed bacteria. We enriched for the viable cells by a combination of lysozyme treatment and filtration and measured the in vitro capacity of their extracts to synthesize polypeptides. Without added template ribonucleic acid (RNA), such "ghost extracts" incorporate amino acids (endogenous synthesis) at approximately one-half the rate as do extracts from uninfected bacteria. However, they are unable to use added synthetic or natural template RNAs for peptide synthesis. Some activity can be observed but only at high concentrations of Mg(2+). These results suggest that ghost infection may result in a blockage of ribosomes during translation. Mixing experiments show that the incapacity of ghost extracts to translate added template RNA is due to a defect in the ribosomes.


Wild-type gross leukemia virus: classification of soluble antigens (GSA). By inhibiting techniques using indirect immunofluorescence tests and indirect immunoelectron microscopy, the G(Gross) soluble antigens (GSA) in the body fluids of AKR and C58 mice, which have a high incidence of spontaneous leukemia, were classified according to the known specificity of G antigens in the murine Gross leukemia system. GSA existing in the plasma of nonleukemic and leukemic AKR mice and in the ascitic fluid of transplanted AKR spontaneous leukemia K36 showed the several specificities corresponding to G cell surface antigens, GCSAa, b, and c, and type-specific and group-specific viral envelope antigens, tsVEA and gsVEA, respectively. However, the plasma of nonleukemic C58 mice lacks GSAc, which can be recognized by the G-typing mouse serum. GSA corresponding to G(IX) antigen was not detected in the body fluids.


Genetic studies on inbreeding in some Japanese populations. I. Introductory remarks.


Genetic stability of Sabin strain of type 3 poliovirus in the sensitivity to inhibitory bovine serum. I. Selection of inhibitory bovine serum against type 3 Sabin strain and sensitivity to the serum of vaccine progenies.


Reflections on paediatric prescribing.

Reflections on paediatric prescribing.


The surgery of primary melanoma: problems and practice.


Let's be practical about a nursing career.


Selected bibliography on associate degree nursing education.


A DNA-unwinding protein isolated from Escherichia coli: its interaction with DNA and with DNA polymerases. A DNA-unwinding protein has been purified to homogeneity from E. coli. This protein has a molecular weight of about 22,000, as judged by its electrophoretic mobility on polyacrylamide gels containing sodium dodecylsulfate, and it appears to be present in about 800 copies per log-phase cell. It binds tightly and cooperatively to single-stranded DNA, and much less tightly, if at all, to RNA or double-stranded DNA. Like the T4 gene-32 protein characterized previously, the E. coli DNA-unwinding protein depresses the melting temperature of double-stranded DNAs, with regions rich in A-T base-pairs being preferentially melted. The E. coli protein strongly stimulates in vitro DNA synthesis by E. coli DNA polymerase II on appropriate templates; however, no stimulation is found with purified polymerases I or III of E. coli, or with T4 DNA polymerase. In contrast, gene-32 protein stimulates only the T4 DNA polymerase in a parallel assay.


Structure and function of Escherichia coli formylmethionine transfer RNA: loss of methionine acceptor activity by modification of a specific guanosine residue in the acceptor stem of formylmethionine transfer RNA from Escherichia coli. The structural requirements of E. coli formylmethionine tRNA for aminoacylation have been examined by chemical modification of the tRNA, followed by separation of the modified molecules into active and inactive components. Photooxidation of tRNA(fMet) at 50 degrees in the presence of methylene blue results in modification of two guanosine (G) residues in the acceptor stem, at positions no. 2 and no. 71 from the 5'-phosphate terminus. Both of these modifications are present in inactive molecules, but only the G residue at position no. 2 is modified in the acceptor stem of active molecules. Loss of methionine acceptance occurs with first-order kinetics, indicating that inactivation by modification of G residue no. 71 is independent of any other modifications taking place under these conditions. The presence of a modified G residue at position no. 2 in the acceptor stem of active photooxidized molecules shows that disruption of normal base-pairing in this region is not sufficient to inactivate tRNA(fMet). These data indicate that the inactivating modification at position no. 71 is lethal due to a specific alteration in the nucleotide base, rather than simply as a result of breaking a hydrogen-bonded base pair in the acceptor stem.


Altered sequences changing the operator-binding properties of the Lac repressor: colinearity of the repressor protein with the i-gene map. A technique is described for mapping point mutations in the first 59 amino-acid residues of the lac repressor from Escherichia coli, using less than 0.1 mumol (4 mg) of the purified protein. This technique was used to localize five mutations affecting the ability of the i-gene product to repress in vivo. These alterations are located at four different sites in the amino-terminal region of the repressor molecule. Three of these are missense mutations and result in changes from serine to proline (residue 16), threonine to alanine (residue 19), and alanine to valine (residue 53). Each amino-acid substitution alone is sufficient to eliminate repression in vivo, presumably by altering the operator binding activity. The remaining two independently-isolated mutations are identical, and result in a change from a glutamine codon at position 26 to an amber (UAG) codon. Since suppression of this nonsense mutation with amber suppressors that insert leucine, tyrosine, serine, or glutamine restores repressor activity to the molecule, glutamine(26) cannot be crucial for the operator-binding function. A comparison of the position of each altered residue with the genetic map enabled us to estimate the physical distance between several deletion-group endpoints.


Two heat-resistant, low molecular weight proteins from Escherichia coli that stimulate DNA-directed RNA synthesis. Two heat-stable protein factors, designated as H(1) and H(2), have been purified from a DNA-protamine sulfate complex obtained in an early step in the preparation of E. coli RNA polymerase. Gel electrophoresis under denaturing conditions indicates that H(1) and H(2) behave as pure entities, with molecular weights below 10,000. Their purity is confirmed by aminoacid composition data, and in the case of H(1), by immunological assays. H(1) and H(2) both strongly stimulate transcription of DNA from bacteriophages lambda and varphi80 by the E. coli polymerase holoenzyme; no effect was observed with single-stranded templates. That the amount of H(1) required for maximal stimulation is proportional to the amount of DNA present in the assay, and that both H(1) and H(2) strongly bind to native DNA in a synergestic fashion, suggests that these low molecular weight factors stimulate RNA synthesis by modifying the properties of the DNA template.


The thiol group in the catalytic chains of aspartate transcarbamoylase. The allosteric enzyme aspartate transcarbamoylase (EC 2.1.3.2) was previously shown to consist of two functionally distinct types of polypeptide chains. X-ray diffraction and chemical studies showed that there are six copies of both catalytic (C) and regulatory (R) chains, and that the intact molecular complex (C(6)R(6)) has D(3) symmetry. Organomercurials react preferentially with the four thiol groups on each R chain, dissociating the molecular complex. We show that 2-chloromercuri-4-nitrophenol reacts specifically and rapidly with the single C-chain thiol, which is believed to be near the catalytic site. This reaction inactivates the enzyme in solution and does not dissociate the molecular complex. Spectrophotometric titration and mercury analysis indicates that six molecules of this mercurial are firmly bound to the enzyme (R(6)C(6)), and crystallographic studies establish that only six sites, related by D(3) symmetry, are modified. The known low reactivity of this C-chain thiol with other sulfhydryl reagents, the unusual structural requirements in the reaction with 2-chloromercuri-4-nitrophenol, and the spectral properties of the resulting derivative provide insight into the environment of this thiol. Probably, at least one positively charged group of the enzyme is nearby, and the environment of this thiol is at least partially hydrophobic.


Purification and properties of D protein: a transcription factor of Escherichia coli. D factor is protein that stimulates DNA transcription in vitro by RNA polymerase. For DNA from bacteriophage lambda, we present evidence that D protein interacts with DNA to increase the frequency and improve the specificity of initiation by RNA polymerase.


Crystals of phosphorylcholine-binding Fab-fragments from mouse myeloma proteins: preparation and x-ray analysis. Fab-fragments of several phosphorylcholine-binding mouse-myeloma proteins have been prepared by pepsin digestion; two of these, MOPC 167 and McPC 603, gave large crystals from ammonium sulfate solutions. The Fab-fragment from MOPC 167 crystallizes in a hexagonal space group, but does not diffract to a resolution greater than about 8 A. In contrast, McPC 603 crystals (space group P6(3)) diffract to about 2.7 A. An isotopic double-labeling technique was developed that demonstrated that the 603 crystals bind 1 mol of hapten per mol of Fab-fragment, but with a binding constant significantly lower than that observed in solution. The findings indicate that a three-dimensional model of this homogeneous antigen-binding immunoglobulin can be constructed. Accordingly, a search for heavy-atom derivatives and determination of the primary structure are in progress.


Eight transfer RNAs induced by infection of Escherichia coli with bacteriophage T4. Bacteriophage T4 induces the synthesis of eight transfer RNAs upon infection of E. coli. The tRNAs are easily detected and resolved into pure species by polyacrylamide gel electrophoresis of RNA labeled with (32)P after T4 infection. Two-dimensional fingerprints of RNase T(1) products derived from individual gel bands give patterns characteristic of single tRNAs. Furthermore, the T(1) digest of each gel band has a single oligonucleotide that contains the minor nucleotides Tp and Psip, a characteristic feature of all known tRNAs. Four larger RNAs are also seen in the polyacrylamide gels. Fingerprint and genetic analyses demonstrate that these molecules are related to the tRNAs, but the exact nature of this relationship is not known.


The three-dimensional structure of yeast phenylalanine transfer RNA: shape of the molecule at 5.5-A resolution. Three isomorphous heavy-atom derivatives have been obtained of orthorhombic crystals of phenylalanine transfer RNA from yeast. These derivatives contain osmium, samarium, and platinum. The positions of the heavy atoms have been determined; these have been used to calculate a three-dimensional electron-density map of transfer RNA at a resolution of 5.5 A. The map shows a high contrast between the molecular boundaries and the solvent areas, so that most of the external shape of the molecule can be determined. The molecule appears to be 92 A long and to have a width varying from 16 A to 34 A. There are some narrow regions in the molecule that connect more globular regions. The electron density map shows chains of dense objects approximately 6 A apart that are probably due to adjacent phosphate groups on the polynucleotide chain. At the present stage of the analysis it is not possible to trace the entire backbone unambiguously; however, the data at this resolution suggest no apparent similarity between the folding of the molecule and any of the tertiary structure models proposed for transfer RNA.


Isolation of a mutant of Salmonella typhimurium dependent on D-arabinose-5-phosphate for growth and synthesis of 3-deoxy-D-mannoctulosonate (ketodeoxyoctonate). A new type of auxotrophic mutant of Salmonella typhimurium has been isolated that is defective in the synthesis of the 3-deoxy-D-mannooctulosonate (ketodeoxyoctonate) region of the lipopolysaccharide and requires D-arabinose-5-phosphate for growth. Genetic and biochemical evidence indicated that the mutant defect was due to an altered ketodeoxyoctonate-8-phosphate synthetase with an apparent K(m) for D-arabinose-5-phosphate 35-fold higher than that of the parental enzyme. As a result of this enzymatic lesion, the mutant strain was dependent on exogenous D-arabinose-5-phosphate both for growth and for synthesis of a complete lipopolysaccharide.


Structural and functional properties of ribosomes crosslinked with dimethylsuberimidate. To test whether a 30S ribosomal subunitformylmethionyl-tRNA-mRNA complex is an obligatory intermediate in protein synthesis, 70S ribosomes from Escherichia coli were crosslinked with the bifunctional imidoester, dimethylsuberimidate. Crosslinked ribosomes contained covelently joined 30S and 50S subunits, as judged by their inability to dissociate at low Mg(2+) concentrations. Treatment of 70S ribosomes with high salt (1 M NH(4)Cl), either before or after reaction with the crosslinking reagent, produced two different crosslinked ribosomal particles, one of "60 S" and the other "70 S." Preliminary evidence indicates that both particles can bind N-acetylphenylalanyl-tRNA at low Mg(2+) concentrations and are active for polyphenylalanine syntheses. Crosslinked ribosomes were functional when tested with poly(U) as an mRNA in systems requiring initiation factors and N-acetylphenylalanyl-tRNA for activity. Under optimal crosslinking conditions, they retained 80% of the activity of unmodified ribosomes for polyphenylalanine synthesis. Despite the maintenance of these functional capacities, such ribosomes had a sharply reduced ability to bind fMet-tRNA and were completely inactive in protein synthesis with bacteriophage f2 RNA as a messenger. We conclude that 70S ribosomes must dissociate into subunits to initiate protein synthesis with natural mRNAs.


Naturally-occurring anti-M and anti-N with special case: IgM anti-N in a NN donor.


High frequency positive pressure ventilation during anaesthesia and routine surgery in man.


Release of polypeptide chain initiation factor IF-2 during initiation complex formation. Polypeptide chain initiation factor IF-2 binds to 30S ribosomal subunits. This binding is enhanced by IF-1 and IF-3. During GTP-dependent formation of a 70S initiation complex, IF-2 is released from the ribosome. During 70S initiation complex formation dependent on the methylene analogue of GTP, GMPPCH(2)P, IF-2 is not released, but remains bound to the 70S ribosome. This result suggests that IF-2 release requires GTP hydrolysis. In agreement with this presumed requirement, IF-2 functions catalytically with GTP, but stoichiometrically with GMPPCH(2)P, in bringing about 70S initiation complex formation.


Drugs, malnutrition, and carbohydrate absorption.


Effects of antibiotics and other agents on the intestinal transport of iron.


Use of the inorganic sulfate sulfur-creatinine ratio in field studies.


Human placental circulation. A preliminary study of the use of silicone rubber injection of the fetal and maternal blood vessels.


Rational therapy for the prolapsed vagina.