Current concerns of the consultant dietitian. II. Sharing information by means of the patient's medical record.


Differential interference contrast for transmitted-light (Nomarski method).


Principles involved in impression making.


An introduction to denture simplification. Phase II.


Zinc silicophosphate cement: influence of composition on the acid solubility and fluoride content of enamel.


A combined chew-in-one-piece casting technique for fixed restorations.


Lingual bar design.

Lingual bar design.


An electron microscopical study of avian muscle spindles.


Exact anhydride: epoxy percentages for araldite and araldite-epon embedding.


Mast cells in the epithelial layer of human gingiva.


Langerhans cells in the ruminal epithelium of the sheep.


Homocellular and heterocellular gap junctions in Limulus: a thin-section and freeze-fracture study.


The fine structure of the contractile apparatus of the anterior byssus retractor muscle of Mytilus edulis.


Electron microscopic observations of the distribution of acidic anionic residues on hamster spermatozoa and eggs before and during fertilization.


Veterans Administration study of coronary bypasses.


Androgens and erythropoiesis.

Androgens and erythropoiesis.


Control of heparin therapy with an activated partial thromboplastin time.


Pneumatoceles associated with Escherichia coli pneumonias in the newborn.


Fables for the instruction of youth published in Boston in 1818. Excerpt XII: Lion and the mouse.


Systemic hypertension following ocular administration of 10 per cent phenylephrine in the neonate.


Warren R. Sisson, M.D., President of the American Academy of Pediatrics, 1948-1949.


Presentation of the Jarvie-Burkart Award.


HL A typing, mixed leukocyte reactivity, and skin graft survival in a family with a recombinant at the HL-1 chromosomal region (major transplantation region).


Cross reactions between human transplantation antigens and bacterial lipopolysaccharides.


Role of the thymus in the proliferation and migration of donor marrow cells in irradiated allogeneic host mice.


Human kidney preservation and shipping.

Human kidney preservation and shipping.


Tributes to Isidore Teich.

Tributes to Isidore Teich.


Origin of cells repopulating the spleens of irradiated mice given marrow cells and thymocytes.


A microgranulocyte cytotoxicity test.

A microgranulocyte cytotoxicity test.


Antilymphocyte globulin as the sole immunosuppressant for bone marrow transplantation in aplastic anemia. A case report.


Survival of Ag-B incompatible parental strain rat renal allografts after splenectomy and xenogeneic antilymphocyte serum pretreatment.


Plasma lipids following renal transplantation.


Renal carcinoma in a living kidney graft donor.


Survival of dog kidney allografts after in vitro irradiation.


Experimental pathology of noise-induced hearing loss.


Experimental studies of otolith function.


Experiments on ototoxic effects of antibiotics.


Autonomic components of the 8th nerve.

Autonomic components of the 8th nerve.


Central aspects of drugs for motion sickness and vertigo.


Anatomical, behavioral, and electrophysiological observations on chinchillas after long exposures to noise.


SEM (scanning electron microscope) studies of aortic structure.


Button inplants re-designed.

Button inplants re-designed.


Comparative investigations on the effect of nicotinic acid alone and in association with a synthetic, heparin-like sulfated polyanion on fibrinolysis, platelet aggregation and lipidic fractions.


In vitro synthesis of deoxynucleotide kinase, dihydrofolate reductase and deosycytidylate hydroxymethylase from RNA transcripts of T2 phage DNA.


Denturism--professional threat.

Denturism--professional threat.


Heterogeneity of subfragment-1 preparations from myofibril digestion by trypsin.


Uncoupler antagonism of valinomycin induced bilayer membrane conductance.


Replication of the nuclear genome in yeast does not require concomitant protein synthesis.


Isolation of pleiotropic yeast mutants requiring ergosterol for growth.


Nature of the ribosomal binding site for initiation factor 3 (IF-3).


Determination of releasing hormones in subcellular fractions from porcine hypothalamic tissue.


Nondiscrimination of RNA viral message in binding to 30S ribosomes derived from T4 phage infected Escherichia coli.


Segmental flexibility of the S-1 moiety of myosin.


Binding of succinate to aspartate trancarbamylase catalytic subunit. pH and temperature dependence of nuclear magnetic resonance relaxation times.


Enthalpy of reduction of disulfide cross-links in denatured lysozyme.


Interaction of ribonucleic acid polymerase from Escherichia coli with deoxyribonucleic acid. Inhibition of ribonucleic acid synthesis by interaction of luteoskyrin with the transcription complex.


Structural investigation of nitrogenase components from Clostridium pasteurianum and comparison with similar components of other organisms.


Proteins from biologically active ribosomal subparticles of Xenopus leavis.


The use of phase contrast microscopy in the diagnoses of dermatomycoses.


Behavioral effects of drug therapy on psychogeriatric inpatients. I. Chlorpromazine and thioridazine.


Casting metal for Crozat appliances.

Casting metal for Crozat appliances.


A new and simple palate splitting device.


A life supporting appliance.

A life supporting appliance.


The dorsal tilt: an embellishment to any operation for hypospadias.


Anuria secondary to mechanical obstruction caused by a Candida fungus ball.


Living donor nephrectomy.

Living donor nephrectomy.


Transplantation: new technique for cadaveric donor nephrectomy.


Compensatory renal hypertrophy: a morphologic study in transplant donors.


Vesicopyelostomy: a method for urinary drainage of the transplanted kidney.


Single-layer bowel anastomosis in intestinal conduit surgery.


The allograft ureter.

The allograft ureter.


Joseph Cangney Von Thron, M.D. Our 97th President.


Current concepts in the pathogenesis of hyperuricemia.


A new and better way to take impressions without hands.


Solution of prosthetic problems with the Wiron metal fused to ceramic method.


Solution of a prosthodontic problem with a pin implant.


Complete dentures.

Complete dentures.


A technique for constructing full dentures using the Gerber articulator and condyloform teeth. I.


Denturism--a report.

Denturism--a report.


Fear of denturism.

Fear of denturism.


A review of respiratory virology and the spread of virulent and possibly antigenic viruses via air conditioning systems. II.


Viral myocarditis and its sequelae.

Viral myocarditis and its sequelae.


Mechanics of air flow in airway obstruction.


Methadone treatment of heroin addiction.


Estimation of gestational age in the fetal state.


Transfer factor therapy in immune deficiency states.


Platelet function tests in disease.

Platelet function tests in disease. Abnormal platelet function may result from congenital or acquired disturbances in the platelets themselves or their plasma milieu in patients with hemorrhage or thrombosis.  Although standardization is difficult and results overlap between patient groups, certain tests are abnormal in patients with diabetes and atherosclorosis, and in women on oral contraceptives, all of whom have an increased tendency toward  thrombosis.  In women on oral contraceptive pills containing synthetic estrogen and progesterone, fibrin formation starts earlier and develops more rapidly when assessed by the thrombelastograph, and as in late pregnancy, Factors VII and X are increased.  Most studies did not report an increase in platelet adhesiveness or in platelet retention measured in whole citrated blood.  An increase in platelet sensitivity to ADP, as measured by electropheretic mobility was observed in 1 study.


Blood levels of parathyroid hormone in disorders of calcium metabolism.


Mental effects of L-dopa.

Mental effects of L-dopa.


Endogenous carbon monoxide metabolism.

Endogenous carbon monoxide metabolism.


Human prolactin.

Human prolactin.


Metabolism of ketone bodies by the brain.


Current concepts in the treatment of chronic myelocytic leukemia.


Preventive antibiotic management in surgery.


Normal and abnormal alpha cell function.


Extraction of insulin by liver.

Extraction of insulin by liver.


Primary hyperlipoproteinemia in infants.


Nature and biological activities of degradation products of fibrinogen and fibrin.


Clinical pharmacology of drug interactions.


The Lesch-Nyhan syndrome.

The Lesch-Nyhan syndrome.


Genetics of congenital heart disease.

Genetics of congenital heart disease.


An approach to the mechanism of killing of cells in suspension by ultrasound.


Delayed embryonic development and implantation in senescent golden hamsters.


A comparison of histological methods for demonstrating calcification.


Injuries to the upper extremity inflicted by the mechanical cornpicker.


The orthopaedic surgery of spina bifida.


A treatise on dislocationa and on fractures of the joints: fractures of the neck of the thigh-bone. Sir Astley Cooper, BART., F.R.S., Surgeon to the King.


A review of biochemical studies on the genetically distinct collagens of the skeletal system.


Simplified rubber base impression technic.


Sterols and fatty acids of some aquatic phycomycetes.


The influence of repeatable castings and melting procedures on volume fraction of voids and corrosion products in case of specified dental and non specified gold alloys.


The tongue: a prosthetic view.

The tongue: a prosthetic view.


The effect of investing media on tooth movement.


Inhibitors of protein synthesis in eukaryotes: tools in cell research.


On the mechanisms of inhibition of polypeptide synthesis by the antibiotics sparsomycin and pactamycin.


Intra-arterial infusion for head and neck cancers.


Toward better health care for the elderly patient.


Suppression: a subversion of genetic decoding.


Genetics and dermatology or if I were to rewrite Cockayne's Inherited Abnormalities of the Skin.


Hyperbaric oxygen therapy in injured subjects.


Molecular aspects of genetic recombination and their relevance to cutaneous biology.


Case assigment in man by somatic cell genetics.


Differences in human pigmentation: measurement, geographic variation, and causes.


Genetic disorders of hair growth.

Genetic disorders of hair growth.


X-linked recessive disorders: chronic granulomatous disease and Wiskott-Aldrich syndrome.


The Chediak-Higashi syndrome and the homologous trait in animals.


Ichthyosiform dermatoses.

Ichthyosiform dermatoses.


Crush injuries of the hand and fingers. 3.


Parkinson's disease; modern treatment.

Parkinson's disease; modern treatment.


History of medicine. The drama of sulfanilamide, penicillin and other antibiotics 1936-1972.


The hormonal and non-hormonal control of renal excretion of calcium and magnesium.


Jewish ethics in relation to medicine.

Jewish ethics in relation to medicine.


Platelet survival test. A method to detect preimmunization in rabbits.


Platelet survival test. An accurate prediction of hyperacute rejection of renal allografts in rabbits.


Studies on the sexual difference in the "spontaneous" production of anti-(human)-A antibodies in C57BL 10 mice.


A heterophile system in human renal transplantation. II. Relationship to clinical renal transplantation and the HL-A system.


Specific unresponsiveness to skin allografts in mice. I. Properties of tissue extracts and their synergistic effect with antilymphocytic serum.


Effects of variable Bacillus Calmette-Guérin regimens on skin graft rejection.


Mechanisms of action of topically applied triamcinolone acetonide in prolonging skin allograft survival time.


Studies on the development of extramedullary marrow implants. Establishment of allografts in thymectomized rats treated with antilymphocyte serum.


125 I-labeled rat transplantation alloantibody. I. Preparation.


Mismatched HL-A haplotypes with antigens HL-A1, 3, and 11 associated with excellent renal allograft function.


Immunological enhancement of renal allografts in the rat. 3. Role of the spleen.


Histocompatibility antigens on the surface of cultivated epidermal cells from mouse embryo skin.


Thymus-specific antigens on human thymocytes and on thymic-derived lymphocytes.


Dose response studies in passive enhancement on rat renal allografts.


Gastrocolic fistula as a complication of gastric lymphoma.


Julius B. Richmond, M.D. President, American Orthopsychiatric Association.


Research findings on the kibbutz adolescent: a response to Bettelheim.


Measurement of thymine dimers in DNA by thin-layer chromatography.


Rapid scanning of  32 P-labeled acrylamide gels.


A rapid assay procedure for thiamine-binding protein of Escherichia coli.


Reversed-phase separation of aminoacyl-transfer RNA's on capillary columns.


Lichenoid tissue reactions. A speculative review of the clinical spectrum of epidermal basal cell damage with special reference to erythema dyschromicum perstans.


A clinical evaluation of 0.05 per cent desonide cream. A new nonfluorinated topical corticosteroid.


Multiple cutaneous squamous cell carcinomas during immunosuppressive therapy.


Bullous systemic lupus erythematosus in a patient.


Psychotherapeutic treatment for rheumatoid arthritis one thousand years ago.


Directory of Arizona Medical Association, Inc.


The influence of mitochondria on ribosomes. Cycloheximide resistance of ribosomes from petite mutants of Saccharomyces cerevisiae.


An active-site peptide from human triose phosphate isomerase.


The calcium dependent endonuclease activity of isolated nuclear preparations. Relationships between its occurrence and the occurrence of other classes of enzymes found in nuclear preparations.


Preparation of pyridoxal 5'-phosphate-bound sepharose and its use for immobilization of tryptophanase.


Substrate specificity of Escherichia coli peptidyltransferase at the donor site.


Double-strand scission of DNA involved in thymineless death of Escherichia coli 15 TAU.


Hydroxylamine-dependent reactions catalyzed by a lysyl-tRNA synthetase of Escherichia coli B.


A lipoprotein from the membranes of Streptococcus pyogenes and its stabilized L-form.


Studies on aspartyl-tRNA synthetase from Baker's yeast. I. Purification and properties of the enzyme.


Energy-dependent masking of substrate binding sites of the lactose permease of Escherichia coli.


Medical supplies for prisoners in 1675.

Medical supplies for prisoners in 1675.


Physiochemical characterization of ribosome-like (55-S) particles from rat liver mitochondria.


The measurement of the ATP-dependent nuclease in crude extracts of wild-type and RecA cells of Escherichia coli.


Stonewall Jackson and his surgeon, Hunter McGuire.


Medical numismatic notes. XII. Bethlem Royal Hospital.


Systemic reactions to topically applied drugs. Howard Fox memorial lecture.


Carlo Gesualdo, Prince of Venosa: murder, madrigals, and masochism.


An essay on the philosophy of medical science.


The activity of individual molecules of hybrid  -galactosidase reconstituted from the wild-type and an inactive-mutant enzyme.


Yeast L-lactate dehydrogenase (cytochrome b 2 ). Chemical characterization of the heme-binding core.


Studies on the conformation of yeast pyruvate decarboxylase in solution by small-angle x-ray measurements.


The kinetics of renaturation of 5-S RNA from Escherichia coli in the presence of Mg 2+  ions.


A study of several bonds hypersensitive to proteases in a complex flavohemoenzyme, yeast cytochrome b 2 . Modification of their reactivity with ligand-induced conformational transitions.


Covalent binding of lipid to protein. Diglyceride and amide-linked fatty acid at the N-terminal end of the murein-lipoprotein of the Escherichia coli outer membrane.


Preparation in vitro of a 2-thiocytidine-containing yeast tRNA Phe -A 73 -C 74 -S 2 C 75 -A 76  and its interaction wiith p-hydroxymercuribenzoate.


Inhibition of protein biosynthesis in Escherichia coli B by tri-L-ornithine.


Inhibition of T4 maturation by tri-L-ornithine.


Linguatula serrata (Pentastomida) parasitizing humans and animals in Egypt, neighbouring countres, and elsewhere: a review.


Ascription of maturity to human figure drawings by preschool children.


Patterns of culturally defined sex-role preference and parental imitation.


Familial variables related to the expression of violent aggression in preschool-age children.


Directory of otolaryngologic societies.

Directory of otolaryngologic societies.


Rhinoplasty tip ptosis: etiology and prevention.


The effects of KC1 concentration on the transcription by E. coli RNA polymerase. I. Specific effect of the combination of nucleoside triphosphates.


The effects of KC1 concentration on the transcription by E. coli RNA polymerase. II. Effect on the specificity of T7 transcription.


The effect of a histidine operator-constitutive mutation on UV-induced mutability within the histidine operon of Salmonella typhimurium.


The mutator property of uvr502 mutation affecting UV sensitivity of Escherichia coli.


Fertility inhibition in strains of Salmonella typhimurium.


A hybridization assay specific for ribosomal RNA from Escherichia coli.


Neurenteric cyst (a case report).

Neurenteric cyst (a case report).


Guanidine derivatives in medicine.

Guanidine derivatives in medicine.


Creutzfeldt-Jakob disease: an electronmicroscopic study with demonstration of virus-like particles.


Recircularization and autonomous replication of a sheared R-factor DNA segment in Escherichia coli transformants. Controlled shearing of R-factor DNA leads to formation of fragments carrying an antibiotic resistance gene present on, but not expressed by, the intact R-factor. Transformation of CaCl(2)-treated E. coli by such fragments yields an autonomously replicating tetracycline-resistance plasmid (Tc6-5) that contains only a small fraction of the genome of the parent R-factor, and lacks both its fertility functions and its other drug-resistance determinants. Although the Tc6-5 plasmid is not self-transmissible, it can interact and/or recombine with conjugally-proficient plasmids that promote its transfer to other bacteria.


Metabolism of membrane phospholipids and its relation to a novel class of oligosaccharides in Escherichia coli. Phosphatidylethanolamine is metabolically stable in exponentially growing cells of Escherichia coli, whereas phosphatidylglycerol and cardiolipin (diphosphatidylglycerol), labeled by growth in the presence of sn-[2-(3)H]glycero-3-(32)P, lose their radioactivity during subsequent growth on unlabeled medium. We have found that the loss of label from the lipid fraction is accompanied by its appearance in a novel type of cell constituent of E. coli, a closely related group of oligosaccharides, each containing glycerol and phosphoric acid in equimolar proportions, probably derived as intact glycerophosphate units from phosphatidylglycerol and/or cardiolipin. Glucose is the sole detectable sugar. The glycerophosphate-containing oligosaccharides may be fractionated by ion-exchange chromatography into several subfractions, some of which contain succinic acid in ester linkage. Although apparently derived from membrane phospholipids, the newly discovered substances contain no fatty acids, are soluble in water, and are insoluble in organic solvents such as chloroform. They comprise about 0.5-1% of the dry weight of growing cells.


Accessibility of DNA in chromatin to DNA polymerase and RNA polymerase. The accessibility of DNA in chromatin to both exogenous DNA polymerase and RNA polymerase is slight when compared to isolated DNA. DNA in extracted chromatin is somewhat more accessible to these enzymes than is DNA in the chromatin of isolated nuclei; and the DNA template of chromatin is more accessible to DNA polymerase than to RNA polymerase. In these experiments we have given much attention to the technique of scintillation counting, since artifacts arising in this procedure can lead to erroneous conclusions.


Synchronous mating in yeast.

Synchronous mating in yeast. Homogeneous a and alpha unbudded yeast cells in logarithmic phase, grown in supplemented minimal medium and isolated by zonal gradient centrifugation, are used for mating. When these cells are resuspended in aerated defined medium, highly synchronous mating rapidly occurs. Within 20 min of incubation at 30 degrees early sexual pairing is evident; extensive agglutination is observed by 60 min, and cell fusion and bud initiation in zygotes occurs after 60-140 min. Sorbitol gradient fractionation of mating mixtures taken at various times during incubation allows the isolation of zygotes or unmated cells. Zygote preparations 90-95% purified are obtained in quantities suitable for genetic and biochemical analysis. The mating procedure is predictable and reproducible.


Mapping of Escherichia coli ribosomal components involved in peptidyl transferase activity. The method of affinity labeling has been used to identify protein components of 50S ribosomal subunits involved in peptidyl transferase activity. E. coli 50S ribosomal subunits were mapped by reaction with the N-bromoacetyl analog of chloramphenicol, an antibiotic known to interact specifically with the active center of the enzyme. The synthetic analog competes with chloramphenicol in binding to 50S ribosomal subunits and inhibits peptidyl transferase activity. It attaches covalently to the ribosome under appropriate conditions and causes an irreversible loss in peptidyl transferase activity. The reagent specifically alkylates cysteine residues of proteins L2 and L27.


Evidence for an amino-terminal extension in high-molecular-weight collagens from mature bovine skin. Insoluble, mature collagen fibers from bovine skin have been partially solubilized by mild, denaturing, but nonhydrolytic means. The soluble denatured collagen was fractionated by alcohol coacervation, and a fraction rich in high-molecular-weight alpha-chains was obtained. The heavy alpha-chains were isolated by carboxymethylcellulose chromatography. Renaturation, followed by measurements of optical rotation at 365 nm, showed that stable, in-register renaturation was more readily accomplished in mixtures of heavy alpha-chains than in alpha1-beta(11)-chain mixtures. Renatured heavy alpha-chain preparations were precipitated in SLS form, negatively stained, and examined by electron microscopy. The SLS precipitates were compared with SLS segments from native soluble collagen and were found to match in band pattern and spacing along their entire length from the COOH-terminal region, except for an NH(2)-terminal extension of 170 +/- 30 A in the heavy alpha-chain SLS. The heavy alpha-chains correspond chromatographically with those previously reported to be intermediates in the conversion of procollagen to collagen, on the basis of their molecular weight and of labeling studies. The presence of NH(2)-terminal extensions, and their existence in mature insoluble collagen, suggest that these intermediates may have a special role in fibril formation.


Association of the rIIA protein with the bacterial membrane. Cell membrane proteins synthesized after infection of Escherichia coli B with wild-type phage T4 and rIIA mutants were analyzed by dodecyl sulfate-polyacrylamide gel electrophoresis. A protein with an approximate molecular weight of 74,000 is present in membranes isolated from T4r(+)-infected cells, but is not found in membranes prepared from cells infected with an rIIA mutant in which the major part of the rIIA cistron is deleted. In addition, infection of E. coli B with different rIIA amber mutants and deletions gives peptides, which are associated with the bacterial membrane, of molecular weights consistent with the location of the respective mutations in the cistron. The rIIA protein is synthesized with delayed early kinetics. The synthesis of the rIIB protein, which is also located in the membrane, is not affected by mutations in the A cistron; conversely, synthesis of the rIIA protein is not affected by mutations in the B cistron. A mutant (rII 1589) contains a deletion that originates in the A cistron and extends into the adjacent B cistron. This mutant directs the synthesis of a compound membrane protein consisting of the undeleted portions of the A and B cistrons. The synthesis of the compound protein appears to be under the control of the A promoter.


Differentiation of lens tissue from the progeny of chick retinal pigment cells cultured in vitro: a demonstration of a switch of cell types in clonal cell culture. Clonal cell lines isolated from the pigmented retina of 8.5-day chick embryos initially retained the capacity to form pigment. After several passages, however, many cells lost pigment granules and differentiated into lens-like structures. The lens-specific nature of the structures formed in cultures originally derived from retinal pigment cells was established by both ultrastructural and immunological studies.


Topographical location of H-Y antigen on mouse spermatozoa by immunoelectronmicroscopy. H-Y (male) antigen was visually located on mouse sperm by electron microscopy, by use of the indirect hybrid antibody method and tobacco mosaic virus as the visual marker. Labeling was achieved by centrifugation of the sperm through a discontinuous gradient consisting of alternating layers of immune reagents and wash solutions. Treated sperm were examined topographically by preparation of platinum-carbon replicas. Antigen was located mainly on the acrosomal cap of the sperm head.


Tropism of Mycoplasma gallisepticum for arterial walls. The fatal encephalopathy associated with M. gallisepticum strain S6 in turkey poults was completely curable by treatment with tylosin or pleuromutilin. The lesions of cerebral polyarteritis disappeared after therapy. Polyarthritis developed in some birds after recovery from encephalitis. Immunofluorescent studies revealed that intravenously injected organisms became localized as microcolonies within the walls of cerebral and periarticular arteries, and in the glomeruli. Turkey IgG deposition was demonstrated on the glomerular basement membranes, but not in the arterial lesions. The results suggest that the pathologic changes caused by this microorganism are due to selective localization and growth in arteries.


T7 early RNAs are generated by site-specific cleavages. Transcription of T7 DNA by purified Escherichia coli RNA polymerase without added factors produces long RNA molecules that begin near the left end of T7 DNA and terminate at the end of the early region. An endonuclease has been isolated from uninfected E. coli that cleaves these long RNAs at five specific sites to generate RNA molecules essentially the same as the early T7 RNAs observed in vivo. This sizing factor, which may be RNase III, can act during or after RNA synthesis. Synthesis of early RNA chains has been shown to start at three strong initiators, spaced about 150-200 base-pairs apart near the left end of T7 DNA. Thus, the five cleavages by sizing factor generate the five early messenger RNAs of T7 plus three overlapping RNAs from the promoter region. RNA chains that are started at two of the strong initiators begin with A; those started at the third begin with G. A few minor initiators have also been observed, from which only short chains seem to be synthesized. Their locations in T7 DNA have not been mapped. Rho factor does not appear to be needed to generate any of these early T7 RNAs.


Synthesis of guanosine tetra- and pentaphosphate requires the presence of a codon-specific, uncharged transfer ribonucleic acid in the acceptor site of ribosomes. The nucleotides ppGpp and pppGpp accumulate when wild-type, but not rel(-), strains of Escherichia coli are starved for required amino acids. These compounds are synthesized on ribosomes, in the presence of the product of the rel gene, from GDP and GTP; ATP is used as the phosphate donor. The signal for making these compounds is the presence of an uncharged tRNA in the ribosomal acceptor site. These compounds are not accumulated if the ribosomes are actively engaged in protein synthesis.


Papers on psychopharmacological research from the Japanese literature.


Curable lung disease in malignant lymphoma.


The effects of benzodiazepines on aggression: reduced or increased?


The cytotoxic action of human lymphocytes from graft recipients on donor target cells in tissue culture.


In memoriam. John Howe Symington.

In memoriam. John Howe Symington.


Pictorial perception and culture.

Pictorial perception and culture.


Comparison of partially purified H-2 antigens extracted with four ionic agents by microcomplement fixation.


Enhancement-induced specific nonreactivity in experimental kidney transplantation.


Cellular and humoral immunity after allogeneic transplantation in the rat. I. Cellular and humoral immunity of measured by a  51 Cr cytotoxicity assay after allogeneic tumor and renal transplantation.


Apparent HL-A5 deficiency in malignant melanoma.


Detection of H-Y (male) antigen on mouse lymph node cells by the cell to cell cytotoxicity test.


Synergism between alloantibodies or F(ab') 2  and prednisone in the survival of murine split heart allografts.


Tereotactic operations for Parkinsonism in Ullevaal Hospital, Oslo.


The influence of L-dopa on physical treatment of Parkinsonism.


Newer diagnostic and therapeutic methods in meningitis and encephalitis.


Virological views on infections in the central nervous system.


Detection of muscle antibodies in myastenia gravis sera.


Immunochemical and immunohistochemical studies of brain tissue in subacute sclerosing panencephalitis and multiple sclerosis.


Surgical treatment, past and present, in Parkinson's disease.


Subacute spongiform encephalopathy: clinical neurophysiological and pathohistological findings.


Avodiance response after lesions of dorsal hippocampus in cats.


Recent advances in the treatment of Parkinsonism with drugs.


Studies on the role of protease inhibitor from potato bulbs in digesting protein food.


Pulmonary thromboembolism: current concepts.


Therapeutic uses and side effects of L-dopa.


Recognition and management of pesticide toxicity.


Arrhythmias and antiarrhythmic drugs.

Arrhythmias and antiarrhythmic drugs.


Non-invasive methods of cardiac diagnosis.


The clinical endocrinology of prolactin.


Therapeutic constraints in hepatic insufficiency.


Transmission electron microscopy of critical point dried tissue after observation in the scanning electron microscope.


Mechanisms of drug-induced hemolytic anemia.


Phase transitions in cells, membranes, and lipids of Escherichia coli. Detection by fluorescent probes, light scattering, and dilatometry.


Endonuclease II of Escherichia coli. Degradation of double- and single-stranded deoxyribonucleic acid.


Caloric catastrophe.

Caloric catastrophe.


Lymphosarcoma in the horse: a review.

Lymphosarcoma in the horse: a review.


Inhibition of the synthesis of macromolecules in Escherichia coli by nitrofuran derivatives. I. (5-Nitro-2-furyl)vinylpyridines.


Inhibition of the synthesis of macromolecules in Escherichia coli by nitrofuran derivatives. II. Various nitrofuran derivatives.


Inhibition of the synthesis of macromolecules in Escherichia coli by nitrofuran derivatives. 3. Nifurpirinol.


Evidence that mutations are induced by errors in repair and replication.


Genetic and physiological separation of the repair and mutagenic functions of the exrA gene in Escherichia coli.


Utilization of aminopurine deoxynucleoside triphosphate by mutator, antimutator and wild-type DNA polymerases of bacteriophage T4.


Population changes in continuously growing mutator cultures of Escherichia coli.


Mutator gene studies in Escherichia coli: the mutT gene.


Genetic recombination induced by DNA cross-links in repressed phage lambda.


E. coli mutants produced by the insertion of bacteriophage lambda DNA.


Ultraviolet mutagenesis in polA and UvrA polA derivatives of Escherichia coli B-R: evidence for an inducible error-prone repair system.


Coagulation problems.

Coagulation problems.


Whither paracervical block?

Whither paracervical block?


"Quinquevalent" nitrogen and the structure of ammonium salts: contribution of Alfred Werner and others.


Routine immunofluorescent microscopy in renal diseases.


Richard Harrison Shryock 1895-1972.

Richard Harrison Shryock 1895-1972.


Influence of diffusion on dispersion of indicators in blood flow.


Assembly of Escherichia coli 50 S ribosomes from ribonucleic acid and protein components. I. Chemical and physical factors affecting the conformation of assembled particles.


Seryl transfer ribonucleic acid synthetase from Escherichia coli. Substrate binding and chemical modification of cysteinyl residues.


Deoxyribonucleic acid photoreactivating enzyme from Escherichia coli. Purification and properties.


Regulation of biodegradative threonine deaminase. II. Reaction mechanism as analyzed by spectrophotometric properties.


Unidirectional transport activity mediated by the galactose-binding protein of Escherichia coli.


The nucleotide sequence of the GGG-specific glycine transfer ribonucleic acid of Escherichia coli and of Salmonella typhimurium.


The stability of acyl carrier protein in Escherichia coli.


The nucleotide sequences and coding properties of the major and minor lysine transfer ribonucleic acids from the haploid yeast Saccharomyces cerevisiae  S288C.


Protein synthesis in rabbit reticulocytes. Assays, purification, and properties of different ribosomal factors and their roles in peptide chain initiation.


Nucleoside triphosphate-nucleoside diphosphate transphosphorylase (nucleoside diphosphokinase). 3. Subunit structure of the crystalline enzyme from brewers' yeast.


Mucosal morphology and cellular renewal in the intestine of chickens following a single infection of Eimeria acervulina.


Experimental toxoplasma infection in mice with strains producing oocysts.


Use of various buffers for in vitro cultivation of malarial parasites.


Functional impression technique for complete denture construction with silicone elastomer.


The importance of anaerobic bacteria in infectious diseases.


Hypoplastic anemia. Pathophysiology and therapy.


The newer antibiotics.

The newer antibiotics.


Hemophilia.

Hemophilia.


Oxygen therapy in pulmonary disease. Oxygen use and toxicity.


Benign disorders of the esophagus. Presentation, diagnosis, and treatment.


Management of the hyperlipidemic patient.


Changes in the spectrum, diagnosis and management of bacterial and fungal endocarditis.


Ultrasound cardiography in clinical practice.


The His bundle electrocardiogram.

The His bundle electrocardiogram.


Principles of drug therapy of hyperlipidemic xanthomatous diseases.


Practical possibilities for lowering serum cholesterol. Results of a double-blind study.


Pathogenesis of cardiogenic shock. II.

Pathogenesis of cardiogenic shock. II.


Role of elastic tissue in cholesterol deposition in the arterial wall.


Lipid composition of xanthomas of different types.


Immune reaction manifesting with anterior uveitis and urogenital disease: prostaglandins as haptens.


Experimental heterotopic liver transplantation.


Intraocular histocompatibility and adhesive strength of butyl-2-cyanoacrylate. Prospective value in retinal detachment surgery.


Serratia marcescens endocarditis: successful carbenicillin and gentamicin treatment.


The president. The American College of Physicians.


Failure of topical amphotericin B in cryptococcosis.


M. Glenn Koenig, M.D., F.A.C.P.

M. Glenn Koenig, M.D., F.A.C.P.


The lungs and pancreatitis.

The lungs and pancreatitis.


Papovavirus of JC type in progressive multifocal leukoencephalopathy. Rapid identification and subsequent isolation.


Left ventricular end-diastolic and filling pressures in assessment of ventricular function.


Role of keratinases in dermatophytosis. II. Fluorescent antibody studies with keratinase II of Trichophyton mentagrophytes.


Hepato-atrial dissociation. Report of two cases and clinical implications.


Temperature-sensitive mutants for the replication of plasmids in Escherichia coli. I. Isolation and specificity of host and plasmid mutations. Temperature-sensitive mutants of Escherichia coli defective in the replication of the plasmid colicinogenic factor E1 (ColE(1)) were isolated following mutagenesis of E. coli K12 strain carrying the ColE(1) factor. Following the mutagenic treatment an enrichment procedure utilizing the replacement of thymine with bromouracil in the ColE(1) DNA duplicated at the restrictive temperature was used. The mutants isolated following this enrichment step were the result of a mutation event either in the host chromosome or in the ColE(1) plasmid. The host mutants fell into three phenotypic classes based on the effect each mutation had on the maintenance of a variety of other extrachromosomal DNA elements. Phenotypic class I mutations affected all E. coli plasmids, both the I and F sex factor types as well as the ColE(1) factor. Phenotypic class II mutations affected the maintenance of the ColE(1) and the F sex factor type plasmids and not the I type, while phenotypic class III mutations affected only ColE(1) replication. None of these mutations was found to have a significant effect on the replication of the E. coli chromosome. The plasmid-linked mutations fell into two phenotypic classes on the basis of the ability of the Flac episome to complement the mutation in the ColE(1) plasmid.


A quantitative ultrastructural study of regeneration from isolated proximal stumps of transected unmyelinated nerves.


Temperature-sensitive mutants for the replication of plasmids in Escherichia coli. II. Properties of host and plasmid mutations. Host mutations in Escherichia coli K12 selected for the temperature-sensitive replication of the bacterial plasmid colicinogenic factor E(1) (ColE(1)) exhibit a pleiotropic effect with respect to the effect of the mutation on other extra-chromosomal elements. The mutations also vary with respect to the time of incubation of the cells at 43 degrees C required for complete cessation of ColE(1) DNA synthesis. While the synthesis of the bacterial chromosome appears unaffected, supercoiled ColE(1) DNA replication stops immediately in some mutants and gradually decreases during several generations of cell growth before stopping in others. Mutations isolated in the ColE(1) plasmid resulted in only a gradual cessation of ColE(1) DNA synthesis over several generations of cell growth at 43 degrees C. Conjugal transfer of the ColE(1) and ColV factors occurs normally in the host mutants when the transfer is carried out at the permissive temperature; however, the presence of a group I mutation in the donor cell prohibited conjugal transfer of either plasmid DNA at 43 degrees C to a normal recipient cell. Similarly, the presence of this mutation in the recipient prevented the establishment of ColE(1) or ColV in the mutant recipient cell upon conjugation with a normal donor at 43 degrees C. Various host ColE(1) replication mutants carrying either ColE(1) or ColE(2) were also defective in the mitomycin C-induced production of colicin E(1) or colicin E(2) at 43 degrees C. The majority of the host mutations examined exhibited a temperature sensitivity to growth in deoxycholate in addition to the inhibition of plasmid DNA replication, suggesting a membrane alteration in these mutants when grown at the restrictive temperature.


Mutagenicity of dimethylnitrosamine in the metabolic process by rat liver microsomes.


Humoral factors of "spontaneous" mutagenesis in mammals and man.


Recent research in EEG amplitude analysis.


Perception of relative size of authority figures and subjects figure by sociopaths and normals.


Psychophysiological effects of extremely low frequency electromagnetic fields: a review.


The optic tracts of Rana temporaia and a possible retino-preoptic pathway.


Hypothalamic mediation of sinus baroreceptor-evoked muscle cholinergic dilator response.


The role of mitochondrial adenine nucleotide pool on the regulation of fatty acid and -ketoglutarate oxidation.


Effect of hypoxia on hypothalamic mechanisms.


Renal regulation of sodium excretion. Function in health and in edema-forming states.


Hydroxyproline stabilizes the triple helix of chick tendon collagen.


Diuretics in nonedematous states. Physiological basis for the clinical use.


A nitrosated arginine derivative, a powerful mutagen.


Induction of the allantoin degradative enzymes by allophanic acid, the last intermediate of the pathway.


Tumor-specific immunity and the Otolaryngologist.


Effect of a bifunctional imidoester on dissociation of 70S ribosomes in Escherichia coli.


Stimulation of DNA synthesis by adenosine diphosphoribosylation of HeLa nuclear proteins during the cell cycle.


Syntheses and biological evaluation of analogs of luteinizing hormone-releasing hormone (LH-RH) modified in position 2, 3, 4 or 5.


Control for vacuum embedding methods in temporal bone histopathology.


Mitochondrial mutants of the yeast Saccharomyces cerevisiae showing resistance in vitro to chloramphenicol inhibition of mitochondrial protein synthesis.


The role of granulocytes in endotoxin-induced vascular injury.


A case of bullous pemphigoid and figurate erythema in association with metastatic spread of carcinoma.


Pemphigus and malignancy.

Pemphigus and malignancy.


A review of the development of the health services of Rhodesia from 1923 to the present day. 3. The war years.


Vibrosis: toward an understanding of immunity in genital infections.


Carcinoma of the colon and rectum.

Carcinoma of the colon and rectum.


Release of LH by LRF injected into the CSF: a transport role for the median eminence.


Effect of synthetic LRF infused into a hypophysial portal vessel on gonadotropin release.


Failure to demonstrate urinary excretion of luteinizing hormone-releasing factor activity in the rat following intravenous injection of porcine hypothalamic extract.


Citation of the Distinguished Leadership Award of The Endocrine Society for 1973 to John S.L. Browne, M.D.


Citation for the Ernst Oppenheimer Memorial Award of The Endocrine Society for 1973 to Richard J. Wurtman, M.D.


Citation for the Koch Award of The Endocrine Society for 1973 to C.H. Sawyer, Ph.D. and J.W. Everett, Ph.D.


Citation for the Eli Lilly Lecturer of The Endocrine Society for 1973 to Hector F. DeLuca, Ph.D.


Citation for the Ayerst Award for Distinguished Service in Endocrinology of The Endocrine Society for 1973 to Robert M. Blizzard, M.D.


The millenary steps of ocular surgery.

The millenary steps of ocular surgery.


Chemical and biological properties of gonadotropin releasing hormone synthesized by the solid-phase method.


Portrait of a great bacteriologisy (Hans Zinsser).


Pyrrolizidine alkaloids--the importance of being metabolized.


Hiatus hernia. Its significance in the elderly patient.


The so called "ideale cholecystektomie" (without drainage) and its place in gallstone surgery.


A new approach to the evaluation of anti-anginal drugs (with special reference to Perhexiline maleate).


A double blind trial of LB-46 in the treatment of angina pectoris.


Ratio of plasma glutaminases in liquid and solid tumour-bearing mice.


Origins and early formulations of the Oedipus complex.


The mode of action of certain drugs against helminth parasites of man.


Control of common carotid and innominate artery hemorrhage complicating tracheostomy.


Peyote: an introduction.

Peyote: an introduction.


Mescaline: the chemistry and pharmacology of its analogs.


Biosynthesis of the peyote alkaloids.

Biosynthesis of the peyote alkaloids.


Antimicrobial effects of aquatic plants from Minnesota.


Treatment of allergic rhinitis with injections of allergenic extracts.


Isolation of induced mutations in E. coli affecting the electrophoretic mobility of enzymes.


Induction of mitotic gene conversion by 3,3-dimethyl-I-phenyltriazene, I-(3-hydroxyphenyl-)-3,3-dimethyltriazene and by I-(4-hydroxyphenyl)-3,3-dimethyltriazene in Saccharomyces cerevisiae.


Effects of an R factor and caffeine on ultraviolet mutability in Salmonella typhimurium.


Evaluation of human health hazards on use of dichlorvos (DDVP), especially in resin strips.


Phage Pl cryptic. II. Location and regulation of prophage genes.


A negative effect of  -glucosylation on T4 growth in certain RNA polymerase mutants of escherichia coli: genetic evidence implicating pyrimidine-rich sequences of DNA in transcription.


The localization of virus-specific double-stranded RNA of cowpea mosaic virus in subcellular fractions of infected Vigna leaves.


Characterization of polycistronic late lambda messenger RNA.


Isolation and characterization of mutants of bacteriophage T4 resistant to folate analogs.


Bacteriophage P1 derivatives with bacterial genes: a heterozygote enrichment method for the selection of p1dpro lysogens.


The immunity reaction of bacteriophage T4: a noncatalytic reaction.


DNA synthesis in cells infected with phage P1c3.


Cucumber mosaic virus contains a functionally divided genome.


The biological synthesis of ascorbic acid.


Mode of action of rifampin on mycobacteria. II. Biosynthetic studies on the inhibition of ribonucleic acid polymerase of Mycobacterium bovis BCG by rifampin and uptake of rifampin- 14 C by Mycobacterium phlei.


Pressure characteristics of a "parachute," self-inflating, intratracheal cuff.


End-to-end anastomosis of the common duct using the Nakayama ring.


Reconstructive arterial surgery: a surgical alternative to amputation.


Physical studies on the effect of chromosomal RNA on reconstituted nucleohistones.


Probabilities in genetic counseling.

Probabilities in genetic counseling.


Progress in the treatment of transposition of the great arteries.


Dr. M. G. Candau and W.H.O.

Dr. M. G. Candau and W.H.O.


Toxoplasmosis.

Toxoplasmosis.


Localization of hepatitis B antigen in liver organ cultures.


Toxicity of benorylate.

Toxicity of benorylate.


Mediterranean anaemia in antiquity.

Mediterranean anaemia in antiquity.


D.L. Savill.

D.L. Savill.


R.S. MacArthur.

R.S. MacArthur.


G.W. Spark.

G.W. Spark.


E.G.R. Grant.

E.G.R. Grant.


Use of coliphages as indicators of water pollution.


Genetics in hypertension. A perspective.


The 1972 Stouffer Prize.

The 1972 Stouffer Prize.


Importance of adaptive changes in vascular design for establishment of primary hypertension, studied in man and in spontaneously hypertensive rats.


Principles of competitive binding assays (saturation analysis). 1. Equilibrium techniques.


Alterations in intrarenal blood flow distribution. Methods of measurement and relationship to sodium balance.


AACC Awards.

AACC Awards.


An application of the temperature reversal effect: a possible method to evaluate donor corneae for penetrating keratoplasty.


Regulation of methionyl-transfer ribonucleic acid synthetase formation in Escherichia coli and Salmonella typhimurium. The control of methionyl-transfer ribonucleic acid (tRNA) synthetase (l-methionine: soluble RNA ligase [adenosine monophosphate]) was studied in methionyl-tRNA synthetase mutants of Escherichia coli and Salmonella typhimurium. The results of activity determinations with crude extracts indicate that this enzyme of the E. coli mutant strain possessed a reduced affinity for methionine-tRNA, whereas this enzyme of the S. typhimurium mutant exhibited a decreased affinity for l-methionine. The differential rate of methionyl-tRNA synthetase formation in these two mutants was several-fold greater than that of the respective parental strains. On the other hand, the level of in vivo aminoacylation of methionine-tRNA was only about one-third that of the parent strains. These results suggest that aminoacylation of methionine-tRNA is a necessary step in repression control of methionyl-tRNA synthetase of both E. coli and S. typhimurium strains.


Deoxyribonucleic acid synthesis in recA and recB derivatives of an Escherichia coli K-12 strain with a temperature-sensitive deoxyribonucleic acid polymerase I. recA and recB derivatives of a strain of Escherichia coli with a temperature-sensitive deoxyribonucleic acid (DNA) polymerase I (polA12) are inviable at high temperature, but continue to incorporate (3)H-thymine into DNA for extended periods. The DNA made in pulse-chase experiments at high temperature in the polA12 parent and its double-mutant derivatives has been examined by alkaline sucrose gradient sedimentation analysis. The low-molecular-weight DNA fragments made during short pulses were joined at the same rate in each strain. Furthermore, the resulting high-molecular-weight DNA was of the same size in each case and was stable for at least 50 min. It is concluded that the inviability of the double mutants is due neither to a defect in converting low-molecular-weight DNA intermediates to high molecular weight nor to the presence of unrepaired random breaks in their DNA.


Distribution of ribosomal ribonucleic acid cistrons among yeast chromosomes. High-molecular-weight deoxyribonucleic acid (DNA) of Saccharomyces carls bergensis has been fractionated by sucrose density gradient centrifugation. The main DNA fraction has an average molecular weight of about 500 x 10(6). A major fraction of the DNA molecules containing sequences homologous to ribosomal ribonucleic acid (RNA) sediments as material of this molecular weight. The remainder sediments as material of a molecular weight of about 250 x 10(6). The latter fraction contains relatively more ribosomal RNA cistrons than the former. Studies on the buoyant density of high-molecular-weight DNA homologous to ribosomal RNA have led to the conclusion that the ribosomal RNA cistrons occur in groups attached to a relatively large amount of nonribosomal RNA and suggest that ribosomal RNA cistrons are distributed over a number of yeast chromosomes.


Inhibition of formation of Escherichia coli mating pairs by f1 and MS2 bacteriophages as determined with a Coulter counter. The effect of male-specific filamentous deoxyribonucleic acid (f1) and isometric ribonucleic acid (MS2) bacteriophages on the formation of mating pairs in Escherichia coli conjugation was examined directly in the Coulter counter. When a sufficient multiplicity of infection (MOI) was used, the f1 phage immediately and completely inhibited the formation of mating pairs. On the other hand, the MS2 phage at a relatively high MOI also inhibited the formation of mating pairs significantly although not completey. The inhibitory effect of MS2 phage was dependent on the time of addition and the MOI used. At relatively low MOI (<20), the MS2 phage showed some inhibitory effect when added to a male culture prior to mixing with females, whereas no effect was observed when phages were added after mating pair formation had already commenced. At a high MOI (>400) MS2 phage disrupted the mating pairs already formed. Some preformed mating pairs were resistant to the high MOI of MS2 phages, however, and the "sensitive" (to high MOI) mating pairs seem to mature into "resistant" mating pairs as a function of time. We conclude that the tip of an F pilus is the specific attachment site for mating. The following process of mating pair formation has been formulated by deduction. (i) The sides of F pili weakly contact female cells, (ii) then the tips of F pili attach to the specific receptor sites to form initial mating pairs, and (iii) those pairs mature into mating pairs that are resistant to the high MOI of MS2 phages. The high MOI of MS2 prevents the first step, whereas f1 phages affect the second step-the binding between the tips of F pili and the receptor sites.


Temperature-sensitive mutants for the replication of plasmids in Escherichia coli: requirement for deoxyribonucleic acid polymerase I in the replication of the plasmid ColE 1 . An Escherichia coli mutant (polA1), defective in deoxyribonucleic acid (DNA) polymerase I, (EC 2.7.7.7) is unable to maintain colicinogenic factor E1 (ColE1), whereas several sex factor plasmids are maintained normally in this strain. polA1 mutant strains containing these sex factor plasmids do not exhibit a readily detectable plasmid-induced polymerase activity. A series of E. coli mutants that are temperature sensitive for ColE1 maintenance, but able to maintain other plasmids, were isolated and shown to fall into two phenotypic groups. Mutants in one group are defective specifically in ColE1 maintenance at 43 C, but exhibit normal DNA polymerase I activity. Mutations in the second group map in the polA gene of E. coli, and bacteria carrying these mutations are sensitive to methylmethanesulfonate (MMS). Revertants that were selected either for MMS resistance or the ability to maintain ColE1 were normal for both properties. The DNA polymerase I enzyme of two of these mutants shows a pronounced temperature sensitivity when compared to the wild-type enzyme. An examination of the role of DNA polymerase I in ColE1 maintenance indicates that it is essential for normal replication of the plasmid. In addition, the presence of a functional DNA polymerase I in both the donor and recipient cell is required for the ColV-promoted conjugal transfer of ColE1 and establishment of the plasmid in the recipient cell.


Role of herpesviruses in Marek's disease, a malignant lymphoma of chickens.


Isolation of deoxyribonucleic acid methylase mutants of Escherichia coli K-12. Fourteen deoxyribonucleic acid (DNA) and 10 ribonucleic acid (RNA) methylation mutants were isolated from Escherichia coli K-12 by examining the ability of nucleic acids prepared from clones of unselected mutagenized cells to accept methyl groups from wild-type crude extract. Eleven of the DNA methylation mutants were deficient in 5-methylcytosine (5-MeC) and were designated Dcm. Three DNA methylation mutants were deficient in N(6)-methyladenine (N(6)-MeA) and were designated Dam. Extracts of the mutants were tested for DNA-cytosine:S-adenosylmethionine and DNA-adenine:S-adenosylmethionine methyltransferase activities. With one exception, all of the mutants had reduced or absent activity. A representative Dcm mutation was located at 36 to 37 min and a representative Dam mutation was located in the 60-to 66-min region on the genetic map. The Dcm mutants had no obvious associated phenotypic abnormality. The Dam mutants were defective in their ability to restrict lambda. None of the mutations had the effect of being lethal.


Lipid synthesis during sporulation of Saccharomyces cerevisiae. Lipid synthesis was studied in both sporulating (diploid) and nonsporulating (haploid) cells of Saccharomyces cerevisiae. Two phases of lipid synthesis occur in diploid cells transferred to sporulation medium. Phase I, which occurs during the first 12 h of exposure to sporulation medium, was also observed in the haploid strains. Phase II, occurring from the 20th to the 25th h, coincided with the appearance of mature asci and was observed only in the diploid cells. The majority of phospholipid synthesis took place during period I, whereas neutral lipid synthesis occurred during both periods. Phospholipid synthesis was virtually identical in both type and quantity in the sporulating and nonsporulating strains.


Reconstitution of nitrate reductase activity and formation of membrane particles from cytoplasmic extracts of chlorate-resistant mutants of Escherichia coli. The reconstitution of nitrate reductase activity in mixtures of cytoplasmic fractions from the chlorate-resistant mutants chlA, B, C, and E which are lacking this activity was investigated, and the membrane-like particulate material which formed during this reconstitution was analyzed by polyacrylamide gel electrophoresis. When chlA and chlB extracts are incubated together, the cytoplasmic membrane proteins present in the particles which are formed are contributed by both mutants, and the proteins are essentially the same as the proteins in the cytoplasmic membrane fractions of the two mutants. Identical amounts of protein become particulate when cytoplasmic extracts of any of the mutant strains or wild-type strains are incubated at 32 C either singly or in mixtures, and the formation of particulate material does not appear to be a consequence of nitrate reductase reconstitution. Experiments with wild-type strains indicate that the membrane proteins in the cytoplasmic extract are derived from the cytoplasmic membrane during cell breakage. Reconstitution experiments involving various combinations of preincubated and unincubated extracts of the mutants have allowed a preliminary identification of three types of components which are necessary for the formation of active nitrate reductase: (i) a soluble factor present only in extracts from induced chlB; (ii) a different soluble factor which is missing in chlB but is present in extracts from wild-type, chlA, chlC, and chlE; and (iii) a complex including the nitrate reductase protein which is inactivated by preincubation of the mutant extracts.


Colicin B: mode of action and inhibition by enterochelin. Adsorption of colicin B to a sensitive strain of Escherichia coli results in rapid cessation of deoxyribonucleic acid, ribonucleic acid, and protein synthesis. Some classes of mutants insensitive to colicin B hyperexcrete a colicin inhibitor into their growth medium. This inhibitor functions by preventing adsorption of colicin B and does not rescue cells to which colicin has already adsorbed. The inhibitor is insensitive to nucleases, proteolytic enzymes, and lysozyme and is not extracted into organic solvents. The inhibitory material has a low molecular weight, which rules out identification as lipopolysaccharide, although purified lipopolysaccharide has some inhibitory activity. Evidence is presented that the inhibitor is enterochelin, an iron chelator which is the cyclic trimer of 2,3-dihydroxybenzoylserine. Enterochelin does not inhibit colicin M, a colicin that is produced by many strains colicinogenic for colicin B.


Excretion of enterochelin by exbA and exbB mutants of Escherichia coli. Escherichia coli mutants that are insensitive to colicins B and I hyperproduce and excrete the iron chelator enterochelin, which is an inhibitor of these colicins. These mutants are classified as exbA and exbB. The exbA mutants are chromium sensitive and require iron for growth, and the mutations are located in the tonB region at min 25 of the E. coli chromosome. tonB mutants in which the genome of phage lambda is inserted into the bacterial chromosome within the tonB gene also exhibit enterochelin excretion. The exbB mutants require methionine and probably result from deletions which are located between min 56 and 58. Colicin insensitivity, enterochelin excretion and methionine auxotrophy are recessive in exbB merodiploids. The methionine requirement of exbB strains is satisfied by cystathionine or homocysteine, and exbB mutants are sensitive to ethionine.


Model for the structure of the shape-maintaining layer of the Escherichia coli cell envelope. The surface area per repeating murein unit (i.e. per molecule of diaminopimelate) has been determined for the cell envelopes of the Escherichia coli strains K-12 and W. This area was constantly found to be 1.3 nm(2). Using this value and other previously determined properties of E. coli murein, a three-dimensional model of murein is proposed. The model specifies a monomolecular layer in which disaccharide units are each 1.03 nm long, and the polysaccharide chains, all parallel, are 1.25 nm apart. The cross-linking peptide side-chains have the same atomic coordinates and are arranged above or below the polysaccharide chains.


Molecular nature of R-factor deoxyribonucleic acid isolated from Salmonella typhimurium minicells. In earlier reports it was shown that a variety of extrachromosomal elements harbored in the Escherichia coli minicell producer segregate into the minicells. We show in this report that the fi(+) R factor R-100-1 (derepressed derivative of R-100) similarly segregates into minicells produced by a Salmonella typhimurium strain. Four distinct classes of covalently closed circular deoxyribonucleic acid molecules are found in minicells derived from the R(+)Salmonella minicell producer. The sum of the average molecular weights or contour lengths of the circular molecules in two of the classes is equal to the average molecular weight or contour length of those in a third class. The data suggest that R-100-1 dissociates into resistance determinants (i.e., genes that specify the molecules that confer resistance) and the resistance transfer factor (i.e., genes responsible for the transferability of the R factor to a recipient). In contrast, only one molecular species is found in minicells derived from the R(+) (R-100-1) Escherichia coli minicell producer. The fourth size class consists of small covalently closed circles (minicircles), which were originally found in the R(-)Salmonella minicell producer and are shown in this report to be enhanced in number in R(+)Salmonella minicells.


Genetic determination of the constitutive biosynthesis of phospho- -glucosidase A in Escherichia coli K-12. Escherichia coli wild-type cells form constitutively the enzyme phospho-beta-glucosidase A, which has a high affinity for phosphorylated aromatic beta-glucosides and a low affinity for phosphorylated beta-methyl-glucoside. Phospho-beta-glucosidase B and beta-glucoside permease I are formed in aromatic beta-glucoside-fermenting mutants. Mutants lacking phospho-beta-glucosidases A and B have been isolated. These mutants showed a reduced rate of inducibility of the beta-glucoside permease I. The restoration of phospho-beta-glucosidase A or B activity resulted in an increased rate of induction of the beta-glucoside permease I. The presence of the phospho-beta-glucosidases was not required for the constitutive biosynthesis of the beta-glucoside permease. Mutants selected for growth on beta-methyl-glucoside as carbon source showed an increased level of constitutive phospho-beta-glucosidase A activity. Gene bglD, the structural gene for phospho-beta-glucosidase A, was mapped between the pyrE locus and the cluster bgl loci, whereas bglE, the regulatory site determining the hyperproduction of phospho-beta-glucosidase A, was mapped between the bgl and ilv clusters. The bglE locus appears to have a regulatory effect on the expression of the bglD gene.


Effects of toluene and phenethyl alcohol on the ultrastructure of Escherichia coli. Treatment of Escherichia coli cells with 0.25% toluene caused partial dissolution of the plasma membrane concomitant with displacement of nuclear material towards the cell periphery. By contrast, treatment with 0.25% phenethyl alcohol generated intracellular membranes.


S-adenosyl methionine-mediated repression of methionine biosynthetic enzymes in Saccharomyces cerevisiae. S-adenosylmethionine (SAM) has been shown to provoke repression of some methionine-specific enzymes in wild-type cells, namely, adenosine triphosphate sulfurylase, sulfite reductase, and homocysteine synthetase. Repressive effects observed in SAM-supplemented cultures should be due to SAM per se, since the intracellular pool of SAM increases while the intracellular pool of methionine remains low and constant. Derepression brought about by methionine limitation is accompanied by a severe decrease in SAM as well as methionine pool sizes, although methionine adenosyl transferase is slightly derepressed. Different hypotheses have been considered to account for the previously reported implication of methionyl transfer ribonucleic acid and the presently reported SAM effects in this regulatory process.


Comparison of the resA1 and polA1 mutations in isogenic strains of Escherichia coli K-12. Strains carrying either the polA1 or resA1 mutation are deficient in DNA polymerase I, and the polA1 and resA1 mutations do not complement in merozygotes. The effect of these mutations in otherwise identical genetic backgrounds was studied: after ultraviolet irradiation both strains degrade their DNA more rapidly and more extensively than the wild-type strains. However, after X-ray irradiation the resA1 strain shows little DNA breakdown and repairs its single-strand breaks. In contrast, the polA1 strain degrades its DNA extensively, and single-strand breaks are not repaired. Moreover, the resA1 strain is capable of supporting the growth of a red(-) bacteriophage lambda, whereas the polA1 strain is not.


Teichoic acid of a stabilized L-form of Streptococcus pyogenes. A stabilized L-form of Streptococcus pyogenes continues to synthesize glycerol teichoic acid. This polymer was obtained from S. pyogenes and its L-form, treated in identical fashion, and compared. Highly purified glycerol teichoic acid from only the L-form was found to be devoid of d-alanine and to have a shorter chain length. Otherwise, the glycerol teichoic acid from these two organisms was found to be a 1,3-phosphodiester-linked glycerophosphate polymer substituted with d-glucose. Evidence is presented that most, if not all, of the glycerol teichoic acid in this streptococcus lies between the wall and membrane. A possible need for the continued synthesis of a minute amount of glycerol teichoic acid by this L-form for survival is discussed in terms of the known function of teichoic acids in bacteria.


Transport of tricarboxylic acids in Salmonella typhimurium. Salmonella typhimurium possesses at least three inducible transport systems for the tricarboxylic acids (citric, isocitric, cis-aconitic, and tricarballylic). The first system was induced by citrate, isocitrate, or cis-aconitate, and transported citric acid and isocitric acid. The second system was also induced by the same acids as in the first system and transported cis-aconitic acid. This system required Mg(2+) ions and was stable at pH 8.4 but unstable at pH 7.0. The metal ion was replaced with Sr(2+) or Ca(2+) ions but not with Ba(2+) ions. The third system was induced by tricarballylate and transported citric acid, cis-aconitic acid, and tricarballylic acid.


Replication of deoxyribonucleic acid during the division cycle of Salmonella typhimurium. The rate of thymidine incorporation into cells of Salmonella typhimurium growing in different media has been measured. In glucose-minimal medium, deoxyribonucleic acid (DNA) replication occurs during the first two-thirds of the division cycle; the final one-third of the division cycle was devoid of DNA replication. The measured doubling time of S. typhimurium in this medium is approximately 48 min, indicating that C (the time for a round of replication) and D (the time between termination and cell division) are approximately 32 and 16 min, respectively. At slower growth rates the pattern of replication is the same as glucose minimal medium. At faster growth rates the "gap" in DNA synthesis disappears. At rapid growth rates evidence for multiple forks is obtained.


Effect of serine hydroxamate on phospholipid synthesis in Escherichia coli. Serine hydroxamate, which inhibits the charging of seryl-transfer ribonucleic acid, reduced the synthesis of phospholipid and nucleic acids in Escherichia coli. This effect was analogous to depriving amino acid auxotrophs of their nutritional requirement and appears to be a manifestation of the stringent response shown by rel(+) strains of E. coli. Amino acid starvation (serine or methionine) alone or serine hydroxamate treatment alone results in 60 to 80% inhibition of lipid accumulation, 90% inhibition of ribonucleic acid accumulation, and an increase in guanosine tetraphosphate (ppGpp). These three effects were reversed by addition of chloramphenicol (CM). A combination of serine starvation and serine hydroxamate treatment resulted in inhibition of lipid and RNA accumulation as well as an increase in ppGpp, but the consequences of the double block were not reversed by CM. We conclude that a strong interrelationship exists among these processes and that CM acts to relax a stringent response by mechanisms other than interference with ppGpp formation. All species of phospholipid were affected by a stringent response evoked by amino acid starvation or addition of serine hydroxamate, but in all cases the synthesis of phosphatidylethanolamine was most severely inhibited. Serine hydroxamate was not incorporated into lipid but specifically caused phosphatidylserine accumulation. Serine starvation produced a dramatic alteration of the distribution of isotope incorporated into phospholipid, which resulted from the stringent response compounded with the limitation of a substrate for phosphatidylserine synthesis.


Ribosomal ribonucleic acid-adenine (N 6 -) methylase of Escherichia coli strain B: ionic and substrate site requirements. These investigations are concerned with the ionic and substrate-site requirements of ribosomal ribonucleic acid (rRNA)-adenine (N(6)-) methylase of Escherichia coli B. The methylase was essentially inactive in solutions of low ionic strength. The addition of MgCl(2) (optimal at 5 mM) or; to a lesser degree, KCl (optimal at 45 mM) stimulated the rate of methylation; the combination of MgCl(2) and KCl stimulated methylation to an extent equivalent to the sum of the stimulation of each acting alone. The extent of nonspecific binding of the methylase to rRNA decreased as the ionic strength of the solution increased. In the absence of ions, dimethylsulfoxide (DMSO), a nucleic acid denaturing agent, had little influence on the rate of methylation; however, DMSO plus KCl synergistically increased both the rate and the extent of methylation to a greater degree than the combination of Mg(2+) plus K(+). NH(4) (+) was less effective than K(+), and the divalent Mg(2+) offered little stimulation. Monovalent anions (acetate, nitrate, and chloride) were equally effective, whereas divalent SO(4) (2-) was decidedly inhibitory. The appropriate ionic milieu of mono- and divalent cations was required to provide the appropriate conformation of the rRNA and to facilitate specific interactions of the methylase and its recognition sites in the rRNA, while decreasing nonspecific ionic binding of the methylase to rRNA. DMSO may facilitate methylation by increasing the number of substrate sites exposed in single-stranded regions of the rRNA. Nonmethylatable rRNA species served as competitive inhibitors, whereas the polyanions deoxyribonucleic acid, transfer RNA, and polyadenylic acid were inactive. Micrococcus lysodeikticus and Bacillus subtilis rRNA, methylated by the methylase, each contained two distinct heptanucleotides containing newly synthesized 6-methyladenine moieties. The data are consistent with the view that E. coli strain B possesses two species of rRNA-adenine (N(6)-) methylases, each of which recognizes a specific adenine moiety in a unique pentapurine nucleotide sequence in a single-stranded region of rRNA.


Effect of iron and salt on prodigiosin synthesis in Serratia marcescens. Serratia marcescens wild-types ATCC 264 and Nima grew but did not synthesize prodigiosin in a glycerol-alanine medium containing 10 ng of Fe per ml. Wild-type 264 required the addition of 0.2 mug of Fe per ml for maximal growth and prodigiosin synthesis; Nima required 0.5 mug of Fe per ml. Three percent, but not 0.1%, sea salts inhibited prodigiosin synthesis in a complex medium containing up to 10 mug of Fe per ml. NaCl was the inhibitory sea salt component. The inhibition was not specific for NaCl; equimolar concentrations of Na(2)SO(4), KCl, and K(2)SO(4) also inhibited prodigiosin synthesis. Experiments with strains 264 and Nima and with mutant WF which cannot synthesize 4-methoxy-2-2'-bipyrrole-5-carboxyaldehyde (MBC), the bipyrrole moiety of prodigiosin, and with mutant 9-3-3 which cannot synthesize the monopyrrole moiety 2-methyl-3-amylpyrrole (MAP) showed that both MBC synthesis and the reaction condensing MAP and MBC to form prodigiosin were relatively more sensitive to NaCl inhibition than the MAP-synthesizing step. The capacity of whole cells to condense MAP and MBC was present, but inactive, in cells grown in NaCl; removal of the NaCl from non-proliferating salt-grown cells restored the activity. Other evidence suggests the existence of a common precursor to the MAP- and MBC-synthesizing pathways.


Acetaminophen versus propoxyphene hydrochloride for relief of pain in episiotomy patients.


Controlled studies of metopimazine for the treatment of nausea and vomiting.


Foreign-body tumorigenesis: no requirement for tissue anoxia.


Soft gelatin capsules. I. Factors affecting capsule shell dissolution rate.


Pharmacokinetics and molecular modification: implications in drug design and evaluation.


Synthesis and antimalarial activity of benzotrifluoride derivatives.


National Health Insurance.

National Health Insurance.


Fluoride uptake by enamel from topical solutions and gels: an in vitro study.


Hypocholesterolemic agents. II. Inhibition of  -hydroxy- -methylglutaryl coenzyme A reductase by arylalkyl hydrogen succinates and glutarates.


Effect of dehydroascorbic acid on permeability of pancreatic islet tissue in vitro.


Dopaminergic neurons: effect of antipsychotic drugs and amphetamine on single cell activity.


Historical perspective: the LPN in Veterans Administration nursing service.


Dr. Donald Kerr receives Distinguished Faculty Achievement Award from University of Michigan.


Behavior.

Behavior.


Radiographic examination of the edentulous patient. I. Review of the literature and preliminary report comparing three methods.


The use of the nursing bottle.

The use of the nursing bottle.


Genetic control of sexual difference.

Genetic control of sexual difference.


Network thermodynamics: dynamic modelling of biophysical systems.


Perry Clement Talkington, M.D. one hundred and first president, 1972-1973.


Eugen Kahn 1887-1973.

Eugen Kahn 1887-1973.


Fluphenazine enanthate and fluphenazine decanoate: a comparison of their duration of action and motor side effects.


Adolf Meyer and psychobiology.

Adolf Meyer and psychobiology.


Mandarin feet.

Mandarin feet.


The hard palate.

The hard palate.


The royal hospitals of the City of London.


Review of the literature.

Review of the literature.


Hybridization of a human lymphoid leukaemia permanent cell line with mouse fibroblast cells.


Dermographism: reduction in weal size by chlorpheniramine and hydroxyzine.


Polymeric collagen.

Polymeric collagen.


The mechanism of action of heterologous anti-lymphocyte sera. I. "In vitro" activity and their effects on different aspects of immunity in rats.


Immunoglobulin and complement bearing lymphocytes in eczema.


Studies on the defective haematopoietic microenvironment of Sl/Sl d  mice.


Adjuvant therapy for adenocarcinoma of the kidney: present position and prospects.


Aneurysms in human renal homografts.

Aneurysms in human renal homografts.


The detection and prediction of acute rejection episodes in human renal transplants using radioactive fibrinogen.


Transection of the bladder with particular reference to enuresis.


A trial of polyglycolic acid sutures for circumcision.


Comparison of two commercially available dipinocula with standard bacteriological assessment of bacteriuria.


Peripartum hepatic dysfunction and xanthogranulomatous pyelonephritis.


Principles of organization of the ventrobasal complex in mammals.


Chemotherapy studies of Salmonella typhimurium in chickens.


An early case of leprosy in a European.

An early case of leprosy in a European.


A review of the development of the health services of Rhodesia from 1923 to the present day. II.


A field trial of the effectiveness of cycloguanil pamoate in Rhodesia.


Clinical experience of amoxycillin in the United Kingdom.


Preliminary clinical study with amoxycillin (BRL 2333) in complicated lower respiratory tract infections.


Stereotaxic-hypothalamotomy.

Stereotaxic-hypothalamotomy.


An assessment of cognitive function in postthalamotomy Parkinson patients.


Hexachlorophane and skin care of newborn infants.


Arginase induction in Aspergillus nidulans. The appearance and decay of the coding capacity of messenger.


Seminar approach for teaching removable partial denture diagnosis.


Pretectal projections to the inferior olive in the rabbit.


Potential uses of prostaglandins in control of reproductive cycles of domestic animals.


Inhibition of the immune response by 7S antibody mechanism and site of action. A cell culture system was used to investigate the mechanism of action of the feedback inhibition caused by specific 7S antibody. It was found that preincubation of spleen cells with specific 7S antibody led to a marked reduction in the in vitro response of the treated spleen cells to the antigen used to prepare the antibody. The inhibition was not caused by a carry-over of free antibody nor by the release of 7S antibody from the cells. Rather, the preincubation appeared to specifically inactivate one of the cells required for initiation of an in vitro response. Since the suppression could be reversed by addition of untreated cells, it was possible to characterize the properties of the reconstituting cell. This cell is identified as the nonlymphoid accessory cell (A cell) by several criteria. (a) Suppression can be demonstrated only in assay systems requiring functional A cells. (b) The most active sources for reconstitution are also good sources for A cells. (c) The sedimentation velocity of the reconstituting cell is identical with that for A cells. (d) Like A cells, the reconstituting cell is resistant to high doses of ionizing radiation. (e) The reconstituting ability is not affected by anti-theta antibody. Of the three cells required for the initiation of an immune response, A cells, bone marrow-derived cells, and thymus-derived cells, the data are only compatible with the reconstituting cell being an A cell. Additional experiments suggest that the Fc portion of 7S antibody binds to the surface of A cells. Thus, fluorescein isothiocyanate-labeled 7S antibody binds specifically to cells with properties similar to those described above, and F(ab')(2) fragments, lacking Fc portion, are unable to cause immunosuppression when they are preincubated with spleen cells. It is possible that this binding is related to the specific suppression caused by 7S antibody molecules.


Antibodies to TRIC agents in matched human tears and sera.


Immunity to chlamydial infections of the eye. I. The role of circulatory and secretory antibodies in resistance to reinfection with guinea pig inclusion conjunctivitis.


Acute destruction by humoral antibody of rat skin grafted to mice.


Age-dependent changes in sensitivity to antigen in the mouse.


Lymphocyte populations: separation by discontinuous density gradient centrifugation.


Mechanism of allograft rejection in New Zealand mice. I. Cell synergy and its age-dependent loss.


Sir Francis Walshe. (1886-1973).

Sir Francis Walshe. (1886-1973).


Robert S. Schwab. (1903-1972).

Robert S. Schwab. (1903-1972).


The relationship between hydramnios and some characteristics of the infant in pregnancies complicated by fetal anencephaly.


The effects of waxed and unwaxed dental floss on interdental plaque accumulation and interdental gingival health.


Responses to the Gold Medal Award presented to the late Dr. Irving Glickman, San Diego, California, October 27, 1972.


Willard Corwin Fleming, D.D.S.,D.Sc. (Hon.), LL.D. (Hon.), 1899-1972.


Host-controlled modification and restriction of bacteriophage T7 by escherichia coli B. T7 phage resists Escherichia coli B host-controlled modification and restriction in vivo, but its DNA carries roughly five sites which are susceptible to the purified enzymes.


Order of injection of T7 bacteriophage DNA. The deoxyribonucleic acid (DNA) corresponding to the left-hand end of the genetic map is injected first in T7 phage infection. The order was deduced by use of marker rescue experiments to see which genes are still injected after the double-stranded DNA chain is broken by X rays. The treatment causes a selective loss of genes located on the right side of the map. Presumably, this corresponds to the tail end of normally injected DNA, and X rays cut off this end.


Replication of bacteriophage phiX174 DNA in a temperature-sensitive dnaE mutant of Escherichia coli C. Bacteriophage phiX174 cannot grow in a temperature-sensitive dnaE (DNA polymerase III) mutant of Escherichia coli C at the nonpermissive temperature. The inability to grow is the result of inhibition of virus DNA synthesis. The synthesis of the parental replicative form is unaffected, but the replication of the replicative form and the synthesis of the single-stranded virus DNA are inhibited.


Role of gene 52 in bacteriophage T4 DNA synthesis. In an attempt to elucidate the mechanism of delayed DNA synthesis in phage T4, Escherichia coli B cells were infected with H17 (an amber mutant defective in gene 52 possessing a "DNA-delay" phenotype). The fate of (14)C-labeled H17 parental DNA after infection was followed: we could show that this DNA sediments more slowly in neutral sucrose than wild-type DNA 3 min postinfection. In pulse-chase experiments progeny DNA was found to undergo detachment from the membrane at 12 min postinfection. Reattachment to the membrane was found to be related to an increase in rate of DNA synthesis. A nucleolytic activity that is absent from cells infected by wild-type phage and from uninfected cells could be detected in extracts prepared from mutant-infected cells. In contrast, degradation of host DNA was found to be less extensive in am H17 compared with wild-type infected cells. Addition of chloramphenicol to mutant-infected cells 10 min postinfection inhibited the appearance of a nuclease activity on one hand and suppressed the "DNA-delay" phenotype on the other hand. We conclude that the gene 52 product controls the activity of a nuclease in infected cells whose main function may be specific strand nicking in association with DNA replication. This gene product might directly attack both E. coli and phage T4 DNA, or indirectly determine their sensitivity to degradation by another nuclease.


Persistent infection of cells in culture by measles virus. 3. Comparison of virus-specific RNA synthesized in primary persistent infection in HeLa cells. The pattern of actinomycin D-resistant RNA synthesis was examined during primary infection of HeLa cells by virulent Edmonston measles virus and in two HeLa clones persistently infected by the same strain of virus. One of these clones, K11, produces infectious virus of low virulence for HeLa cells, and the other, K11A-HG-1, has thus far failed to yield infectious virus. The patterns of virus-specific RNA synthesized in these three types of infection are qualitatively similar to each other and to the patterns of virus-specific RNA synthesis in other paramyxovirus infections. There were, however, quantitative differences. In addition, virions of the virulent Edmonston strain of measles virus were found to contain high-molecular-weight RNA with a sedimentation constant identical to that of Newcastle disease virus.


Prophage induction by high temperature in thermosensitive dna mutants lysogenic for bacteriophage lambda. High-temperature treatment of thermosensitive dna mutants lysogenic for phage lambda leads to prophage induction and release of phage (at the permissive temperature) in elongation-defective mutants of the genotypes dnaB, dnaE, and dnaG. In initiation-defective mutants no prophage induction occurs at 42 C in mutants of the genotype dnaA, whereas with a dnaC mutant as well as with strain HfrH 252 (map position not yet known) phages are released at 42 C. DNA degradation at the replication fork at 42 C is observed in all dnaB(lambda) mutants tested, but not in mutants of the genotypes dnaE(lambda) and dnaG(lambda). Therefore, degradation of replication fork DNA is not a prerequisite for prophage induction.


Reminiscences of the Harriet Lane Home Outpatient Department.


Dr. Edwards A. Park, physician, teacher, investigator, friend.


Curriculum Vitae. Solomon A. Berson.

Curriculum Vitae. Solomon A. Berson.


Solomon A. Berson, M.D. Introduction.

Solomon A. Berson, M.D. Introduction.


Glucose regulation of insulin biosynthesis in isolated rat islets.


Clinical dermatology. 1.

Clinical dermatology. 1.


A personal appreciation (Solomon A. Berson).


Hypernatremic (hypertonic) dehydration in infants. The etiology, epidemiology, pathophysiology, and complications, therapy, and prognosis of hypernatremic (hypertonic) dehydration in infants are briefly discussed.  The most likely causal condition for hypernatremic states in infants is enteric disease, because the symptoms of diarrhea and vomiting result in water loss and inability to take in water for replenishment.  Other causes include dubious feeding practices, diabetes mellitus, diabetes insipidus, and maladroit diagnostic and therapeutic maneuvers, including administration of radiologic contrast medium or hypertonic sodium bicarbonate or mannitol infusions, or the use of salt solutions as an emetic.  Epidemiologically, 2 factors are apparent: high saline diet and winter season.  The clinical hallmarks of hypernatremic disturbance are relative preservation of circulation and early presence of neurologic symptomatology.  Renal tube necrosis is also occasionally encountered.  Therapy is rehydration, but the bone of contention is the technique for replacing water in the face of the fact that water administered without electrolyte causes the brain to swell and frequently results in convulsions.  The management of hypernatremic dehydration begins with a replenishment phase if neither shock nor apparent anuria is present.  The principle is to replenish the body slowly, and 48 hours has been chosen as the target, so that for volume the deficit plus 2 days of ongoing losses should be allocated.  However, the sodium and other ion contents are derived solely from the deficit, without factoring the 2-day maintenance period.  A recipe for rehydration fluid is presented.


By the London Post. Profiteering in drugs?--Selection and training of medical students--William Harvey's revolutionary circus.


Lymphocyte surface immunoglobulins in Hodgkin's disease.


Cadet nurse. The girl with a future.

Cadet nurse. The girl with a future.


The General Hospital in early Singapore. I. (1819-1829).


Antagonism of spironolactone-induced natriuresis by aspirin in man.


Solomon A. Berson: the VA years.

Solomon A. Berson: the VA years.


A comparison of some pharmacological effects of Escherichia coli (E. coli) L-asparaginase and E. coli endotoxin in rabbits.


Histochemical studies during the differentiation of dissociated nerve cells cultivated in the presence of brain extracts.


Cholera in early Singapore. I. (1819-1849).


The Pauper Hospital in early Singapore. I. (1819-1829).


Solomon A. Berson: the Sinai years.

Solomon A. Berson: the Sinai years.


Pathogenesis of genetic diabetes mellitus: further development of a hypothesis.


The role of host RNA polymerase in P1 phage development.


Immunochemical heterogeneity of circulating parathyroid hormone in man: sequel to an original observation by Berson and Yalow.


The development and application of antibodies to gastrin.


Paradoxical pulse: 100 years later.

Paradoxical pulse: 100 years later.


Treatment of anovulation with synthetic luteinizing hormone-releasing hormone.


Correction of prominent ears. A new technique.


Long-term survival of HeLa tumours in mice treated with antilymphocyte serum. During studies of the ability of antilymphocyte serum (ALS) to suppress the immune mechanism of mice and thereby allow HeLa cells to grow into a large tumour in the mice, it was observed that many tumours continued to grow even after the ALS treatment had been stopped and full immunological competence of the mice had returned. The HeLa cells of such tumours appeared to be unchanged in their ability to induce further tumours in ALS treated mice to which they were transferred and, furthermore, the mice which were carrying such tumours in the presence of immunological competence were able to reject additional injections of HeLa cells or other human tumour cells. The four possible explanations for this phenomenon, (i) depression of cellular response; (ii) local reaction at the graft site; (iii) the presence of a blocking factor; and (iv) the elevation of the humoral response, have been investigated.


Immunogenicity and immunosensitivity of urethane-induced murine lung adenomata, in relation to the immunological impairment of the primary tumour host. The depression of the immunological status of BALB/c mice treated during infancy with two different doses of urethane, alone or combined with cortisone, was evaluated by counting the number of plaque forming cells at 30 or 50 days of age. The incidence of lung adenomatous nodules was directly related to the degree of immunological impairment at 50 days of age. Twenty-seven lung adenomata were tested in an in vitro system involving spleen cells immune against the same single tumour used as target cell. Eighty-six per cent of tumours in the most immunodepressed group of mice were positive compared with 20-40% in the less immunodepressed groups. Syngeneic cross-reaction tests showed that non-immunogenic tumours were immunosensitive since 66% positive tests were obtained when target cells belonging to the less immunodepressed groups were tested with spleen cells of mice immunized with immunogenic adenomata.


Cyclophosphamide and nandrolone decanoate in the treatment of advanced carcinoma of the breast--results of a comparative controlled trial of the agents used singly and in combination. A random trial in which cyclophosphamide, nandrolone decanoate and the two drugs in combination were used in the treatment of advanced breast carcinoma is described. The results suggest that it is preferable to use cyclophosphamide on its own.


The emergence of drug resistance in tumours: a characteristic which may be exploited therapeutically.


Hemorrhage in gynecologic surgery.

Hemorrhage in gynecologic surgery.


Bacterial gangrene associated with pelvic surgery.


Management of the pelvic abscess.

Management of the pelvic abscess. 137 consecutive cases of pelvic abscess arising within the female genital tract admitted to Grady Memorial Hospital between January 1968 and December 1968 were studied to compare the outcome of conservative management with primary aggressive  surgical treatment.  The majority of women (about 70%) were between 21 and 40 years of age; 30% were nulliparous and 40% had had a pregnancy within 2 years prior to the pelvic abscess.  120 patients were managed conservatively: bedrest, fluid, and electrolyte replacement, nasogastric suction in 35 of these cases.  The average hospital stay for the 120 patients was 9 days.  There was an early failure rate of 10% who underwent exploratory laparotomy with total hysterectomy and bilateral salpingooophorectomy; rupture of the abscess occurred in approximately 4% of the conservatively managed cases.  16% of the remaining 97 patients followed for 2 1/2-8 years had delayed complications following discharge and required surgery for persistent symptoms or mass.  There was no intestinal or urinary tract injury to these patients requiring surgery.  10 patients treated conservatively have subsequently become pregnant, with no reported ectopic pregnancies.  Among 12 cases of ruptured pelvic abscess, postoperative morbidity was high.  17 cases of the 137 underwent initial laporotomy because of suspected rupture.  These patients had total abdominal hysterectomy.  Primary conservative therapy is favored over aggressive management via surgery, except in cases of questionable diagnosis, suspected rupture, or failure of the abscess to respond to initial conservative management.


Clinically important drug interactions.

Clinically important drug interactions.


On the mechanism of oligonucleotide-primed DNA synthesis.


Tyrosyl-tRNA synthetase of Escherichia coli B. Role of magnesium ions in the reaction catalyzed by the enzyme.


Tyrosyl-tRNA synthetase of Escherichia coli B. Binding of various ligands.


Cell coupling in epithella.

Cell coupling in epithella.


Transport of organic molecules in the lens.


Serum amylase. A comparison of saccharogenic and four commercial dye-substrate methods.


Gold leaf in the treatment of leg ulcers.


Antibody formation to embryonic intestine in syngeneic rats.


Immune response of mice to nucleic acids: strain-dependent differences in magnitude and class of antibody production.


Position of the selective peptic cleavage of a human kappa-chain between the Vk and Ck domains.


Development of primary  M,  G, and  A anti-TNP plaque-forming cell responses in vitro.


Importance of chimerism in maintaining tolerance of skin allografts in mice.


Genetics of the immune response. II. Inheritance of antibody specificity to lipopolysaccharides in mice.


Chain growth rate of  -galactosidase during exponential growth and amino acid starvation.


Regulation of ribonucleic acid synthesis in Escherichia coli B-r: an analysis of a shift-up. 1. Ribosomal RNA chain growth rates.


Synthesis of RNA polymerase in Escherichia coli B-r growing at different rates.


In vitro synthesis of Escherichia coli ribosomal RNA.


Mechanism of transcription of bacteriophage S13. I. Dependence of messengerRNA synthesis on amount and configuration of DNA.


Mechanism of transcription of bacteriophage S13. II. Inhibition of phage-specific transcription by nalidixic acid.


Testicular tumors in children.

Testicular tumors in children.


Urinary incontinence in myelomeningocele.


American Urological Association, Inc.

American Urological Association, Inc.


A technique for individual vessel ligation of the difficult renal pedicle.


An 8-year urological experience in the multidisciplinary approach to the care of the meningomyelocele patient.


Aldosteronism updated.

Aldosteronism updated.


Autotransplantation of the kidney.

Autotransplantation of the kidney.


Extravesical removal of the intramural ureter: a simplified technique.


The ureteropyelostomy in human renal transplantation.


In vitro repression of n- -acetyl-L-ornithinase synthesis in Escherichia coli.


Biogenesis of mitochondria 27. Genetic and biochemical characterisation of cytoplasmic and nuclear mutations to spiramycin resistance in Saccharomyces cerevisiae.


Transient regulation of enzyme synthesis in Escherichia coli.


A fine-structure map of the yeast l-asparaginase gene.


Two new ribosomal precursor particles in E. coli.


Effect of DNA conformation on ribosomal RNA synthesis in vitro.


The foundation of a college. The conception, birth and early days of the College of General Practitioners.


The medical and scientific importance of hallucinogens.


Slow-release iron in anaemia.

Slow-release iron in anaemia.


Three Scots in the service of the Czars. I.


Gaucher's disease. Phillipe Charles Ernest Gaucher (1854-1918).


Infection of an avulsed papillary muscle tip simulating bacterial endocarditis.


Dietary treatment of renal failure.

Dietary treatment of renal failure.


Propranolol.

Propranolol.


Acute high-altitude illness in mountaineers and problems of rescue.


Rheumatoid arthritis, 1970-1972.

Rheumatoid arthritis, 1970-1972.


A simple apparatus for the continuous growth of experimental tumor cells in vitro.


The problem of  -granule ultrastructure in the endocrine pancreas.


Some methods for processing of single-cell protein.


Revascularization of free full thickness skin autografts.


The kinetic behaviour of transfused donor bone marrow neutrophils and their precursors in neutropenic patients.


Positive and expiratory pressure breathing in pulmonary oedema following myocardial infarction.


Double blind trial of prednisone, in low dose, in patients with chronic airways obstruction.


Measurement of drug action in schizophrenia.


Ultrahigh-speed cutting: The coolant controversy revisited.


Upper denture impression technic.

Upper denture impression technic.


Studies on the deposition of inhaled fibrous material in the respiratory tract of the rat and its subsequent clearance using radioactive tracer techniques. 1. UICC crocidolite asbestos.


Desk top calculators and binary coded decision making.


Chemical profiles of disease.

Chemical profiles of disease.


Computer assisted pattern recognition and the diagnosis of liver disease.


Contributions of computer simulation to clinical enzymology.


Effect of immunization with common enterobacterial antigen on experimental Salmonella typhimurium infection of mice.


Anti-DNP hemolytic plaques by mouse spleen cells in diffusion chambers.


Isolation of functional antigen-carrying spleen cells early in the course of the immune response of mice to sheep red blood cells.


Influence of gonadal factors on skin test reactivity of CFW mice to Candida albicans. Sex differences in the delayed skin test response to Candida albicans were studied in gonadectomized and control CFW mice sensitized either actively with sonically treated antigen or passively with lymphoid cells. In mice actively sensitized with antigen, the responses to candida skin testing were larger in the female. In both sexes, the skin test responses were larger in mice gonadectomized prior to sensitization than in controls of the same sex. In mice passively sensitized with male lymphoid cells, the responses to candida skin testing were essentially equal in the sexes. In both sexes, gonadectomy prior to passive sensitization with the male cells resulted in greater skin test reactivity. In mice passively sensitized with female lymphoid cells, the responses were larger in the males. In males, pre-sensitization gonadectomy resulted in smaller skin test responses, but in the females gonadectomy did not influence the responses. It is suggested that the greater skin test reactivity of the female to candida is due to a sex difference in the response of lymphoid cells to gonadal hormones. This difference may be important in the greater immune reactivity and resistance to infection which occur in the female.