Treponema pallidum haemagglutination test for syphilis. Comparison with TPI and FTA-ABS tests.


Treponema pallidum haemagglutination test for syphilis. Evaluation of a modified micro-method.


Treponema pallidum haemagglutination test for yaws. Comparison with the TPI and FTA-ABS tests.


Doxycycline in the single-dose treatment of gonorrhoea. Effect on Treponema pallidum.


History of the treatment of trichomoniasis.


The emergence of the Consultant Venereologist.


Gerald Legh Malins McElligott, M.A. Oxon., M.R.C.S., 1897-1972.


Appearance of new biological properties in populations of protista cells possibly transfected with the genome of an animal virus.


A comparison of methods for the enrichment of salmonellae from sheep faeces.


Idiopathic tarsitis in postparturient dairy cows: clinicopathologic findings and treatment.


Isolation of pectolytic clostridia from potatoes.


A comparative assessment of media for the isolation and enumeration of coagulase positive staphylococci from foods. A report from a working party of the Public Health Laboratory Service.


Comparison of poured and surface inoculated plates of Baird-Parker's medium for enumerating Staphylococcus aureus in foods.


Views on the pathogenesis and management of primary gout--1971.


Lymphoid changes during antibody synthesis in larval Rana catesbeiana.


Stimulation of RNA synthesis in developing frog embryos by microinjection of ribosomes.


Mechanism of replication of phichi174 single-stranded DNA. IV. The parental viral strand is not conserved in the replicating DNA structure.


Mechanism of replication of phichi174 single-stranded DNA. V. Dispersive and conservative transfer of parental DNA into progeny DNA.


The use of a simple test for hypoglucosuria (uriglox) in the diagnosis of bacteriuria in pregnancy.


Acute peritonitis in a kitten associated with Salmonella typhimurium infection.


Polyglycolic acid surgical sutures in gynaecological surgery.


Sam F. Seeley, M.D.

Sam F. Seeley, M.D.


Fractures of the tibia and fibula. A follow-up study of the initial 64 patients treated at the University Hospital, Kuala Lumpur, Malaysia.


Effect of endotoxin on myocardial performance.


Ureteral function in renal transplantation.


Subcortical cancellous bone grafting in the treatment of delayed union of tibial shaft fractures.


The Pereyra procedure and urinary stress incontinence.


External sphincterotomy in boys with meningomyelocele.


Historic hospitals. The Massachusetts General Hospital.


The biogenesis of mitochondria 26. Mitochondrial recombination: the segregation of parental and recombinant mitochondrial genotypes during vegetative division of yeast.


Evidence for bidirectional chromosome replication in Escherichia coli C  based on marker-frequency analysis by DNA-DNA hybridization with P2 and lambda prophages.


The bivious suppressiveness of cytoplasmic petites of S. cerevisiae lacking in mitochondrial DNA.


On the mechanism of conjugation in Escherichia coli K12. 3. Synthesis of DNA in the course of bacterial conjugation.


A complex genetic locus controlling purine nucleotide biosynthesis in yeast.


Amber mutants of Salmonella-phage P22 in genes engaged in the establishment of lysogeny.


Different photodynamic action of proflavine and methylene blue on bacteriophage. II. Mutation induction in extracellularly treated Serratiaphage kappa.


The origin of DNA in transducing particles in P22-mutants with increased transduction-frequencies (HT-mutants).


Experience with multiple burn casualties on the occasion of the Ljubljana air crash 1966.


The immunological follow up of renal allograft recipients.


The treatment of dermal burns.

The treatment of dermal burns.


Induction and suppression of lactation in animals.


Iron-binding proteins in milk and resistance to Escherichia coli infection in infants.


Complement and the kidney.

Complement and the kidney.


Neurosurgical aspects of psychosurgery.

Neurosurgical aspects of psychosurgery.


Psychosurgery today: psychiatric aspects.


The place of chemotherapy in Hodgkin's disease and allied disorders.


Inspector-general of hospitals Robert Jackson (1750-1827), Army Medical Department.


Current studies in surgical shock.

Current studies in surgical shock.


Some aspects of facial nerve paralysis. I. Introduction, aetiology, applied anatomy and topognosis, and degree of paralysis.


Various items from the Museum of the History of Medicine in Johannesburg.


Sweating sickness in England: an alternative interpretation.


Dermatophilosis: a skin disease of animals and man. II.


The Veterinary Investigation Service in England and Wales: half a century of development.


Factors involved in mastitis control: a comparative study between high and low incidence herds.


Loss of antibiotic resistance from bacteria exposed to anthelmintic agents.


Transductional analysis of resistance to lincomycin and erythromycin in Streptococcus pyogenes.


Measurements of alveolar sac diameters by incident-light photomicrography. Effects of positive-pressure respiration.


Tuberculosis in Canadian Eskimos.

Tuberculosis in Canadian Eskimos.


Preventive treatment of untreated, nonactive tuberculosis in an Eskimo population.


Glomerulonephritis associated with hydrocarbon solvents: mediated by antiglomerular basement membrane antibody.


Vestibular and spinal control of eye movements.


Cross coupling between effects of linear and angular acceleration on vestibular nystagmus.


Optomotor integration in the colliculus superior of the cat.


Neuronal elements of the orienting response. Microrecordings and stimulation experiments in rabbits.


Lateral geniculate body structure and eye movement.


Saccade correlated events in the lateral geniculate body.


Supranuclear structures regulating binocular eye and head movements.


The control of eye movements in the saccadic system.


Adaptive control of asccadic eye movement.


Principles of optomotor reactions in insects.


Electronic simulation of the velocity function of movement-detecting neurons.


Investigations of the eye tracking system through stabilized retinal images.


Rabbit optokinetic reactions and retinal direction-selective cells. A preliminary model.


Ambivalent optokinetic stimulation and motion detection.


Eye movements necessary for continuous perception during stabilization of retinal images.


Eye movements and space perception.

Eye movements and space perception.


Visual perception of direction and voluntary saccadic eye movements.


Voluntary eye movements as questions.

Voluntary eye movements as questions.


The neuronal mechanism of nystagmus.

The neuronal mechanism of nystagmus.


How do we see with moving eyes?

How do we see with moving eyes?


Vestibular and cerebellar control of oculomotor functions.


Electrophysiological investigation of the organization of the vestibulo-ocular pathways in rabbit.


Ultrastructural and light microscopic observations of rat pituitary LH-containing gonadotrophs following injection of synthetic LH-RH.


The neural input of the milk ejection reflex in the hypothalamus.


Asbestos bodies, their formation, composition and character.


Advances in male reproductive physiology.


Modifications in the large-scale production of baby hamster kidney cells in roller bottles.


Albumin metabolism.

Albumin metabolism.


A quantiative radioisotope method for the vivo evaluation of cephalic circulation in the rat.


Amino acid sequence of the center of the arginine-lysine-rich histone from calf thymus. The total sequence.


Replicative deoixyribonucleic acid synthesis in a system diffusible for macromolecules.


On the turnover of ppGpp in Escherichia coli.


Identification of a reactive cysteine residue at the glutamine binding site of carbamyl phosphate synthetase.


Studies on polynucleotides. 118. A further study of ribonucleotide incorporation into deoxyribonucleic acid chains by deoxyribonucleic acid polymerase I of Escherichia coli.


Studies on polynucleotides. CXIX. Transcription of short double-stranded deoxyribonucleic acids of defined nucleotide sequences.


Studies on polynucleotides. CXX. On the transcription of a synthetic 29-unit long deoxyribopolynucleotide.


The deoxyribonucleic acid modification and restriction enzymes of Escherichia coli B. I. Purification, subunit structure, and catalytic properties of the modification methylase.


The deoxyribonucleic acid modification and restriction enzymes of Escherichia coli B. II. Purification, subunit structure, and catalytic properties of the restriction endonuclease.


Mechanisms of active transport in isolated bacterial membrane vesicles. VII. Fluorescence of 1-anilino-8-naphthalenesulfonate during D-lactate oxidation by membrane vesicles from Escherichia coli.


Acyl carrier protein. XIX. Amino acid sequence of liver fatty acid synthetase peptides containing 4'-phosphopantetheine.


Nucleotide specificity of Escherichia coli succinic thiokinase. Succinyl coenzyme A-stimulated nucleoside diphosphate kinase activity of the enzyme.


Cold-sensitive mutants of Salmonella typhimurium defective in uridine monophosphate kinase (pyrH).


The biosynthesis of the cross-linking peptides in the cell wall peptidoglycan of Staphylococcus aureus.


A rapid purification procedure for glyceraldehyde 3-phosphate dehydrogenase from Bakers' yeast.


Enzymatic joining of polynucleotides. XI. Reversal of Escherichia coli deoxyribonucleic acid ligase reaction.


Transport of succinate in Escherichia coli. II. Characteristics of uptake and energy coupling with transport in membrane preparations.


Phosphorylation of initiation factor IF-2 from Escherichia coli with skeletal muscle kinase.


Heterogeneity of gonadotropin response to LHRH in hypogonadotropic hypogonadism.


Numerical classification and coding of electrocardiograms.


Use of an on-line computer in a study of cardiac arrhythmia.


Computer-aided ECG analysis and research in a clinical setting.


Oral contraceptives: therapeutics versus adverse reactions, with an outlook for the future I.


Manuel Dagnino.

Manuel Dagnino.


Synthesis and antifungal activity of polyhalophenyl esters of pyridyl- and 4-quinolylcarbamic acids. IV.


An evaluation of immunohistochemical techniques for antibody activity in the gastro-intestinal mucosa. Horseradish peroxidase conjugated IGA and IGM.


Estolate and stearate forms of erythromycin in the treatment of acute beta haemolytic streptococcal pharyngitis.


Complications from wearing soft contact lenses.


Dental aspects of the early and periodic screening diagnosis and treatment program under medicaid.


The effects of reinforcement procedures upon schizophrenic patients: a survey of the data.


Recent developments in the study of locus of control.


The effects of modeling, roleplaying, and participation on behavior change.


The effect of vicarious reinforcement on imitation: a review of the literature.


Uricult dip-slide in significant bacteriuria.


Sylvasun and prevention of sun-trauma.

Sylvasun and prevention of sun-trauma.


Percussion: Von Auenbrugg and Corvisart.


Chronic hepatitis--clinicopathological differentiation between active chronic hepatitis and recurrent or persistent (viral) hepatitis.


Stereotaxic methods in the investigation of experimentally produced intracranial displacements.


Biochemical effects of halothane.

Biochemical effects of halothane.


The challenge of service.

The challenge of service.


Baron Justus von Liebig-chemist-teacher.


The deciduous dentition after only primary surgical operations for clefts of the lip, jaw, and palate.


Stimulus-secretion coupling of glucose-induced insulin release. X. Effect of glucose on  45 Ca efflux from perifused islets.


Mercury pollution.

Mercury pollution.


Forgotten firsts.

Forgotten firsts.


Leukocytic response as a monitor of immunodepression in burn patients.


Postoperative diaphragmatic herniation following transthoracic fundoplication. A note of warning.


Gastric blood flow in endotoxin-induced stress ulcer.


Lung allografts in calves. A correlation of functional changes with the histology of rejection.


Cystic dilation of intrahepatic bile ducts. Presenting as gram-negative septicemia.


The problem of R-factor mediated multiple antibiotic resistance in Arizona.


A kidney transplant patient with Fabry's disease. Unusual features.


A clinical trial with hydrocortisone butyrate cream in eczema.


Limitations of paired comparisons of topical drugs.


Topical aminoglutethimide in hirsutism.

Topical aminoglutethimide in hirsutism.


The dip-slide in urology.

The dip-slide in urology. To assess the value of the Uricult dip-slide in a hospital urological unit, bacteriological examination of 1,033 urine specimens was compared by this technique and by conventional methods. In 87% of cases identical results were obtained using the dip-slides and the standard laboratory culture plates. Insignificant differences occurred in 10%, and a serious discrepancy between the two methods was found in only 3%. The Uricult dip-slide technique is valuable as a bacteriological screening procedure in urological practice but subculturing from the slide is not recommended except in special circumstances.


Liquefaction of sputum by N-acetyl-l-cysteine and dithiothreitol.


Problems with ketamine anaesthesia.

Problems with ketamine anaesthesia.


The detection of anti-nuclear factor using horseradish peroxidase.


Genesis of cardiac arrhythmias.

Genesis of cardiac arrhythmias.


Effect of breathing oxygen in patients with severe pulmonary vascular obstructive disease.


Bacterial aerosols generated during high speed instrumentation: possible means of reduction.


Treating the total patient. Systemic disease symptoms.


The dentist's role in the early detection of rheumatic fever.


Intraoral technic to repair veneer crowns and pontics.


The effect of certain nitrogen mustard derivatives on bone marrow colony-forming units and erythroid repopulating ability. I. Rats.


The effect of certain nitrogen mustard derivatives on bone marrow colony-forming units and erythroid repopulating ability. II. Mice.


Georg von Békésy.

Georg von Békésy.


Some effects of Freud's personality on psychoanalytic theory and technique.


Freud's views on bisexuality and therapy. Clinical notes.


Iowa Dental Association 1973 membership directory. Active and life members.


A reappraisal of the dementia praecox concept. I. Developmental arrest and regression.


Biological activity of pepstatins, pepstanone A and partial peptides on pepsin, cathepsin D and renin.


BB-K 8, a new semisynthetic aminoglycoside antibiotic.


Survey of inhibitors in different steps of protein synthesis by mammalian ribosomes.


Grafts: some intraoral uses.

Grafts: some intraoral uses.


A new enzyme in Escherichia coli carrying R-factor phosphorylating 3'-hydroxyl of butirosin A, kanamycin, neamine and ribostamycin.


Making a good impression.

Making a good impression.


The controversy over end expiratory positive pressure versus negative expiratory phase.


Fixed prosthetics with a natural crown pontic using an adhesive composite. Case history.


Role of the GSA in medical education, 1957-1972.


A synchronized intrathoracic auxiliary heart transplant "in parallel".


Cellular characteristics of the rejection response to canine lung allotransplants.


Gene dosage effect in expression of blood group antigens.


Metabolic fate and mechanism of action of chloroethylthiamine. VI. Mechanism of anticoccidial action of chloroethylthiamine.


Effect of pantothenic acid deficiency on lipid metabolism in yeast. II. Influence of pantothenic acid deficiency on neutral fats and phospholipids.


Capillary constriction in cochlear and vestibular tissues during intense noise stimulation.


Surgical technique in clefts of the lip and palate.


Gaze-evoked movements of upper eyelids and Sir William Gowers.


Comparative study of CNS lesions induced by tick-borne encephalitis virus in normal and x-irradiated white mice.


Bibliography: literature related to use of instrumental aids in stuttering therapy.


Translation of bacteriophage Q  RNA by cytoplasmic extracts of mammalian cells. Cytoplasmic extracts from Krebs II mouse ascites cells and from L cells translate messenger RNA from coliphage Qbeta with fidelity to produce products that migrate on polyacrylamide gels with those products directed by Qbeta RNA in an Escherichia coli cell-free system. The mammalian cell extracts correctly initiate and terminate Qbeta coat protein synthesis, as shown by: (i) [(3)H]lysine-and [(3)H]arginine-labeled tryptic peptides derived from the coat-sized product resemble these from authentic Qbeta coat protein, (ii) Qbeta coat (which contains methionine only at the N-terminal end) can be radioactively labeled with methionine only if the methionine is formylated, and (iii) L cell extracts directed by Qbeta am(-)11 (an amber mutant in the coat protein) RNA make no completed coat-sized material, but do make a peptide the size of the authentic amber coat fragment.


Regulation of macromolecular biosynthesis in a mutant of Escherichia coli defective in membrane phospholipid biosynthesis. Nucleic acid and protein synthesis were studied in temperature-sensitive mutants defective in phospholipid synthesis. The defect is due to a single mutation in glycerol 3-phosphate acyltransferase (EC 2.3.1.15). The results show that at the restrictive temperature not only does phospholipid synthesis cease, but DNA, RNA, and protein synthesis also cease. Active transport continues, however, indicating that the cells do not become leaky or lose their energy supply. These results suggest that phospholipid synthesis is coupled to DNA, RNA, and protein synthesis.


Regulation of the expression of the N gene of bacteriophage lambda. A quantitative assay for the N protein of bacteriophage lambda has been used to study the in vivo regulation of N gene expression. The assay makes use of the observation that in a cell-free protein-synthesizing system from Escherichia coli programmed with lambdaN(-) DNA the lambda endolysin is made only if N protein is added to the reaction. The rate of synthesis of N protein in vivo is negatively controlled by the products of the CI and tof genes of the phage. Furthermore, N protein activity is extremely unstable in vivo. During normal cell growth at 35 degrees , the half-life of N protein is about 2 min.


Three adjacent transfer RNA genes in Escherichia coli. A defective varphi80 transducing phage has been isolated that carries a small section of the E. coli chromosome from the region near argH (77 min). This phage contains three E. coli tRNA genes, termed glyTsu(+) (36), tyrT, and thrT. These genes specify the structures of tRNA(2) (Gly) (su(+) (36)) tRNA(2) (Tyr), and tRNA(3) (Thr), respectively. Induction of a single lysogen bearing this phage brings about selective synthesis and amplification of only these three tRNAs.


Illicit transport: the oligopeptide permease. The oligopeptide permease of Escherichia coli has been characterized by Payne, Gilvarg, and their colleagues. We have confirmed its existence in Salmonella typhimurium, and have isolated a series of mutants lacking the permease. We use this transport system for smuggling a histidine biosynthetic intermediate, histidinol phosphate ester, into the bacteria as its glycylglycyl derivative, Gly-Gly-histidinol phosphate. Free histidinol phosphate ester is not transported into Salmonella. Several amino-acid analogues are shown to be much more inhibitory to Salmonella when presented to the bacteria in the form of tripeptides than as the free amino acids. The implications of this work for practical purposes are discussed. The synthesis of Gly-Gly-histidinol phosphate is described.


Induction of immunoglobulin M synthesis and secretion in bone-marrow-derived lymphocytes by locally concentrated concanavalin A. Stimulation of bone-marrow-derived lymphocytes by locally concentrated concanavalin A involves first an increased intracellular synthesis of total protein and of immunoglobulin M at 10-14 hr, followed by the initiation of secretion of protein and of IgM at 24-30 hr after stimulation. Differentiation into immunoglobulin-producing and -secreting cells after stimulation is manifested by (a) a rate of IgM synthesis that increases continuously over total protein synthesis, and (b) an increased rate of IgM secretion with concomitant decrease of secretion of other proteins. Within stimulated bone-marrow-derived cells, IgM molecules were found mainly in their 7S form, together with some light-mu-heavy-chain precursors. Small amounts of polymerized 19S IgM are found associated with the cells. Only fully assembled 19S IgM was found secreted into the extracellular medium. Polymerization into the pentamer therefore takes place shortly before, or simultaneously with, secretion. Intracellular 7S IgM contains only glucosamine and mannose residues, with traces of fucose and galactose residues. Secreted 19S IgM has the full complement of carbohydrates with glucosamine, mannose, fucose, galactose, and N-glycolylneuraminic acid residues. During polymerization and secretion from the cells, IgM molecules therefore acquire all of the semiterminal and terminal residues of their carbohydrate groups.


LDH-release in various experimental conditions as criterion for viability of the hypothermic perfused kidney.


RNA initiation with dinucleoside monophosphates during transcription of bacteriophage T4 DNA with RNA polymerase of Escherichia coli. The effects of dinucleoside monophosphates on the transcription of phage T4 DNA by E. coli RNA polymerase have been examined at various concentrations of the sigma subunit and extremely low concentration of ribonucleoside triphosphate. The following conclusions were reached: (i) Labeled specific dinucleoside monophosphates are incorporated as chain initiators. (ii) When the ratio of sigma factor to core enzyme is small, there is a general stimulation by most 5'-guanosyl dinucleoside monophosphates. (iii) When the ratio is increased or holoenzyme is present, ApU, CpA, UpA, and GpU are the most effective stimulators. (iv) At high concentrations of sigma factor, only certain adenosine-containing dinucleoside monophosphates (ApU, CpA, UpA, and ApA) stimulate the reaction. (v) Competition hybridization studies indicate that the RNAs stimulated by dinucleoside monophosphates (ApU, CpA, UpA, and GpU) are of the T4 "early" type. (vi) Studies involving both combinations of stimulatory dinucleoside monophosphates and competitive effects of these compounds on chain initiation by ATP and GTP suggest that the stimulatory dinucleoside monophosphates act as chain initiators and may recognize part of a continuous sequence in a promoter region. Studies based on the incorporation of (3)H-labeled stimulatory dinucleoside monophosphates support the above conclusions.


Isolation of substances that stimulate RNA synthesis from red kidney bean: their activities in lymphocytes and Escherichia coli. An extract from red kidney beans (Phaseolus vulgaris) was fractionated with respect to RNA synthesis-stimulating activity. The activity of the fractions was tested in chicken-spleen lymphocytes cultured in serum-free medium and in plasmolyzed E. coli cells. Lymphocyte-stimulating activity was found in a fraction called F III. This fraction contained protein and orcinol-positive carbohydrate. The protein (called F III p) responsible for the observed activity was separated from the contaminating carbohydrate by acid precipitation. F III p appears to belong to the group of well-known mitogens of high molecular weight present in red kidney beans. A bacteria-stimulating factor was found in a fraction called F V. This factor strongly stimulated RNA synthesis in the bacterial system, and had a weak stimulating effect in the lymphocyte system. In plasmolyzed E. coli cells, F V counteracted the inhibiting effect of rifampicin on RNA synthesis.


1973 dental directory (Loma Linda alumni).


The history of medicine in the Orange Free State, 1854-1902.


Immunoglobulin E and atopic diseases.

Immunoglobulin E and atopic diseases.


Potential environmental chemical hazards. I. Drugs.


Management of the appendiceal stump in children.


What's new in surgery. Cardiothoracic surgery.


What's new in surgery. Neurologic surgery.


What's new in surgery. Plastic surgery and burns.


What's new in surgery. Transplantation.

What's new in surgery. Transplantation.


What's new in surgery. Urology.

What's new in surgery. Urology.


The importance of pulmonary artery pressure monitoring.


A technique for the performance of an intestinal anastomosis.


Evaluation of upstream sampling method for quantitating aortic regurgitation in man.


The diagnostic value of ventricular volume measurements.


Physeo-epiphyseal injuries of lower extremities in myelomeningocele.


Aerospace Medical Association. Directory of members.


Peptidoglycan types of bacterial cell walls and their taxonomic implications.


Pedigrees of some mutant strains of Escherichia coli K-12.


Linkage map of Escherichia coli strain K-12.


Escherichia coli K-12 F-prime factors, old and new.


A comparative study of histone acetylation, histone deacetylation, and ribonucleic acid synthesis in avian reticulocytes and erythrocytes.


Circular dichroism studies on the conformation of transfer ribonucleic acid in the presence of different divalent cations.


F + , Hfr, and F' strains of Salmonella typhimurium and Salmonella abony.


Kinetics of ethidium bromide binding as a probe of transfer ribonucleic acid structure.


New trephine for keratoplasty with interchangeable blade.


A dihydroxyboryl-substituted methacrylic polymer for the column chromatographic separation of mononucleotides, oligonucleotides, and transfer ribonucleic acid.


Unequivocal clinical evidence for the oxygen effect.


Linkage map of Salmonella typhimurium, edition IV.


The continuous suture in cataract surgery and its effect on intraocular pressure.


Right aortic arch with mirror-image branching causing vascular ring. A new classification of the right aortic arch patterns.


Colicin E3 induced cleavage of 16S ribosomal ribonucleic acid; blocking effects of certain antibiotics.


Some aspects of the surgical treatment of tumours of the head and neck which involve bone.


Wilhelm Conrad Röntgen, 1845-1923.

Wilhelm Conrad Röntgen, 1845-1923.


Conventional and subtraction scanning of the pancreas: an assessment based on blind reporting.


Passaic County Dental Society membership roster 1972-1973.


A new technique for suturing apically positioned flaps in periodontal surgery.


The Ayurvedic tradition of child care. Pediatric wisdom of ancient India.


On separateness and oneness. An I-self dialogue.


Judgments of psychopathology from art productions of children.


Health hazards from wastewater treatment practices.


Metachromasia of basic dyes induced by mercuric chloride. I.


Studies in lipid histochemistry. X. Lipids in paraffin sections.


Cannulation of the papilla of Vater by endoscopy and retrograde cholangiopancreatography (ERCP).


Carboxyl terminal amino acid sequence of guinea pig   1 G.


Studies on the influence of radiation of Saccharomyces cerevisiae under anaerobic conditions.


Intestinal immunoglobulins in children with coeliac disease. The numbers of immunoglobulin-containing cells in jejunal biopsy specimens of 19 children with active coeliac disease aged 0.5 to 16.5 years were studied by direct immunofluorescence. Intestinal juice immunoglobulins were measured in 14 of these patients. The number of IgA-containing cells was twice and the number of IgM-containing cells 2.5 times that of age-matched controls. There were also more IgG-, IgE-, and IgD-containing cells in the jejunal mucosa of the coeliac patients, but the absolute numbers of these cells were low. The immunoglobulin content of the intestinal juice was not altered in coeliacs.A follow-up biopsy specimen was available from seven patients kept on a strict gluten-free diet for one to four months. A significant fall in the numbers of immunoglobulin-containing cells was seen, and they did not differ at that time from the controls. Two patients were followed until full normalization of the jejunal structure and they had normal numbers of immunoglobulin-containing cells. In children with coeliac disease in contrast to adult coeliacs, the study shows that the IgA-producing system is quantitatively stimulated during gluten challenge. The rapid drop in the numbers of immunoglobulin-containing cells after gluten withdrawal suggests that there is no quantitative abnormality in the local immunoglobulin-producing system of the gut in coeliac disease.


Use of acid etchant on fresh preparations to improve definition of impressions and remove temporary cement. II.


Mannan of yeast bud scars. A comparison of the structure of bud scar mannan with that of the cell wall of Saccharomyces cerevisiae.


Studies on the formation of transfer ribonucleic acid-ribosome complexes. XXIV. Effects of antibiotics on binding of aminoacyl-oligonucleotides to ribosomes.


An estimate of the minimum amount of unsaturated fatty acid required for growth of Escherichia coli.


Loss of methionine acceptor activity resulting from a base change in the anticodon of Escherichia coli formylmethionine transfer ribonucleic acid.


Initiation of deoxyribonucleic acid synthesis. IV. Incorporation of the ribonucleic acid primer into the phage replicative form.


The cytoplasmic maturation of a ribosomal precursor ribonucleic acid in yeast.


The interaction of phosphoglucomutase with nucleotide inhibitors.


Yeast chitin synthetase. Separation of the zymogen from its activating factor and recovery of the latter in the vacuole fraction.


Polyadenylic acid sequences in yeast messenger ribonucleic acid.


Osteo-onychodystrophy with nephropathy and renal osteodystrophy. A case report.


On the role of deoxyribonucleic acid polymerase in determining mutation rates. Characterization of the defect in the T4 deoxyribonucleic acid polymerase caused by the ts L88 mutation.


Multiple pyogenic arthritis due to Serratia marcescens following renal homotransplantation. Report of a case.


The 1973 membership roster.

The 1973 membership roster.


Guillermo Rawson.

Guillermo Rawson.


Solitary cyst of the talus.

Solitary cyst of the talus.


Delayed solitary metastasis to the radius of renal-cell carcinoma.


The effect of saline loading on endotoxin-induced disseminated intravascular coagulation.


The role of obstruction and infection in biliary tract disease.


Isolation of an Escherichia coli strain restricting bacteriophage suppressor.


A protein produced by male strains of Escherichia coli K-12 which increases the yield of recombinants in conjugation: its nature and mode of action.


The problem-oriented record: a critique from a believer.


DNA targets for the Escherichia coli K restriction system analysed genetically in recombinants between phages phi80 and lambda.


New Zealand Medical Research Council standing committee on therapeutic trials.


More reasons why Weed is right.

More reasons why Weed is right.


Penicillamine--an Anglo-American adventure in the treatment of rheumatoid arthritis.


Genetic analysis of mutants of Salmonella typhimurium deficient in formate dehydrogenase activity.


Richard Orgias.

Richard Orgias.


Methionine and vitamin B 12  repression and precursor induction in the regulation of homocysteine methylation in Salmonella typhimurium.


Joseph John Brownlee.

Joseph John Brownlee.


Prevention and management of venous thrombosis and pulmonary embolism.


Abdominal surgery. I.

Abdominal surgery. I.


Indirect ultraviolet induction and curing in E. coli cells lysogenic for bacteriophage lambda.


In vivo translation of amber and ochre codons in Escherichia coli.


Respiratory response to electrical stimulation of the brain stem of fetal and neonatal sheep.


Indicator loss during injection in the thermodilution system.


The early years of the university of the Witwatersrand Medical School and its students.


Patient identification with the I SPY health program (children and youth project).


Some aspects of facial nerve paralysis. 3. Complications, prognosis and management.


Oral pre-anaesthetic medication with a new benzodiazepine hypnotic.


The genetics of hereditary disorders of blood coagulation.


Pyrantel pamoate in roundworm infestations: a comparative trial with piperazine citrate given in a single dose.


The changing face of Texas medicine.

The changing face of Texas medicine.


Id reactions from focal fungus infections treated by immunological methods.


Treatment of urogenital trichomoniasis with klion.


Teperin, a new psychotropic.

Teperin, a new psychotropic.


Clinical and clinico-pharmacological trials with probon, a new analgesic.


A method for preservation of soft tissues of marine teleosts for scanning electron microscopy.


Fixation and embedment, in situ, of tissue culture cells for electron microscopy.


Bite modification appliances--planes, plates and pivots.


Fixation artifacts in oral biopsy specimens.


Iliac bone graft for restoration of avulsion defects of the mandible. A case report.


Dermatophilosis: a skin disease of animals and man. I.


Evaluation of the pyroantimonate technique for localization of tissue sodium.


Blast injuries to the lungs. A report of two cases.


Survival of chronic bronchitis after intermittent positive pressure ventilation.


The New York Academy of Dentistry. Fellowship list.


Examination of patients for prosthesis: especially the aging patients.


Endobronchial intubation in infancy.

Endobronchial intubation in infancy.


Anaesthesia in sickle cell states. A review.


A rapid, presumptive procedure for the detection of Salmonella in foods and food ingredients. A rapid detection procedure was developed in which a lysine-iron-cystine-neutral red (LICNR) broth medium, originally described by Hargrove et al. in 1971, was modified and used to detect the presence of viable Salmonella organisms in a variety of foods, food ingredients, and feed materials by using a two-step enrichment technique. Tetrathionate broth was used to enrich samples with incubation at 41 C for 20 hr, followed by transfer to LICNR broth and incubation at 37 C for 24 hr for further enrichment and for the detection of Salmonella organisms by color change. One hundred ten samples representing 18 different sample types were evaluated for the presence of viable Salmonella. Ninety-four percent of the samples found to be presumptive positive by this method were confirmed as positive by a culture method. Fluorescent-antibody results also compared closely. A second study was conducted under quality-control laboratory conditions by using procedures currently employed for Salmonella detection. One hundred forty-three samples representing 19 different sample types were evaluated for the presence of viable Salmonella. No false negatives were observed with the rapid-detection method. The usefulness of the LICNR broth procedure as a screening technique to eliminate negative samples rapidly and to identify presumptive positive samples for the presence of viable Salmonella organisms was established in this laboratory.


Isolation of Serratia marcescens on deoxyribonuclease-toluidine blue-cephalothin agar. Serratia marcescens was isolated on a new medium-commercial deoxyribonuclease agar with the addition of cephalothin (1,000 mug/ml) and Toluidine Blue (1,000 mug/ml). It was detected in water samples even though it comprised only 0.1 to 0.0001% of the total bacterial population.


Detection of type E botulinal toxin in cultures by fluorescent-antibody microscopy. Vegetative cells of toxigenic Clostridium botulinum type E cultures were stained with fluorescent antitoxin prepared against purified toxin. The staining seems to be specific.


Recognition of Citrobacter diversus in the clinical laboratory. Characteristics and antibiotic sensitivities of Citrobacter diversus are described, and a minimal number of diagnostic tests to identify this organism in a clinical laboratory are suggested.


Effects of homologous bacteriophage on growth of Pseudomonas fragi WY in milk. Pseudomonas fragi strain WY and its homologous bacteriophage were added in varying concentrations to sterile skim milk which was stored at 7 C for 72 hr. When the initial concentration of the bacterial host was 100,000/ml, addition of as few as 10 plaque-forming units per ml of bacteriophage resulted in significantly lower counts in treated skim milk than in the controls which contained no phage. There was no significant effect, however, when the phage input was 1 in 10 ml and the bacterial count was 1,000 or 100,00/ml. No differences in bacterial counts occurred even when the phage concentration was 1,000/ml if the initial bacterial concentration was only 1,000/ml.


Characterization of Staphylococcus aureus in a pediatric burn unit. A one-year study on an endemic strain of Staphylococcus aureus phage type 84/85 in a children's burn unit is described. The endemic strain rapidly colonized the burns and nares of acute patients after admission but was not isolated from a patient on admission. Nonendemic strains of S. aureus found on some new patients were mostly non-phage typable and did not prevail in burns. The endemic strain was rarely isolated from the nares and skin of reconstructive patients or from the nares of hospital personnel. The endemic strain did colonize the oral cavity, normal skin, and intestinal tract of some acute patients. Endemic and nonendemic strains of S. aureus from the burned children were compared in their biochemical activities and antibiotic sensitivities to two groups of S. aureus from one other local and one Danish burns unit. The latter groups of strains represented different combinations of staphylococcal phage group III strains. Each of the four groups of strains differed in production of hemolysins, Tween 80 hydrolysis, egg yolk reaction, and proteolysis of casein and gelatin. All of the strains were uniformly sensitive to gentamicin, oxacillin, and cephalothin. Only 4 of 162 strains tested were methicillin resistant. The endemic S. aureus strains of phage type 84/85 were uniformly resistant to eight other antibiotics including lincomycin and clindamycin. The endemic strain was not the known cause of a clinically documented infection in a group of 82 acute patients studied. The possible role of S. aureus strains of phage group III in burn grafting problems is discussed.


Cellulolytic bacteria associated with sloughing spoilage of California ripe olives. Sloughing spoilage of California ripe olives during processing is characterized by severe softening, skin rupture, and flesh sloughing. It was assumed that cellulolytic activity was responsible for skin rupture and sloughing of flesh, and so a deliberate search was made for cellulolytic bacteria from olives undergoing sloughing spoilage. A bacterium identified as Cellulomonas flavigena was highly cellulolytic, attacking filter paper, carboxymethyl cellulose (CMC) gel, and olive tissue. Other bacteria attacking CMC, but not filter paper, enhanced the activity of the Cellulomonas strain when grown in mixed culture, although they did not, in pure culture, have any effect on filter paper. These latter cultures (all degraded olive tissue) represented the genera Xanthomonas, Aerobacter, and Escherichia. Other noncellulolytic bacteria belonging to the genera Alcaligenes, Kurthia, and Micrococcus also were used for study of mixed culture fermentation of cellulose by C. flavigena. Cellobiose accumulation at levels of 1.0% (w/v) and above suppressed growth of C. flavigena.


Comparison of three blood culture media for recovery of anaerobic bacteria. Comparison of an anaerobic brain heart infusion broth with tryptic soy broth and Thioglycollate medium failed to demonstrate significant differences in isolation rates of anaerobic bacteria from blood.


The prepaid group practice plan in the education of future physicians: initial efforts at the Harvard Community Health Plan.


Presence of thermophilic bacteria in laundry and domestic hot-water heaters. Thermophilic bacteria resembling Thermus aquaticus were isolated from hot water taken from domestic and commercial hot-water tanks. Cold water from the same locations never yielded thermophilic bacteria, suggesting that the bacteria were growing in the tanks. In contrast to the T. aquaticus isolates from hot springs, the present isolates were rarely pigmented. In general, the hotter sources more frequently yielded bacteria.


Comparison of methods for isolation of anaerobic bacteria from clinical specimens. Five different anaerobic culture methods and several different media were compared for their ability to recover anaerobes from clinical specimens. Specimens were obtained from patients with documented infections, avoiding contamination with normal flora, and immediately placed in an anaerobic transporter. Each specimen was cultured by all methods and on all the various media. The comparative data indicate that anaerobic jars (GasPak and evacuation-replacement types) are just as effective in the recovery of clinically significant anaerobes as the more complex roll-tube and chamber methods employing prereduced media. Liquid media were disappointing as a "back-up" system but chopped-meat glucose was superior to two thioglycolate formulations. Growth of all anaerobes was poorer on selective media, but these media were very helpful in the workup of specimens containing mixed growth of anaerobic and facultative organisms. A variety of different anaerobes was isolated, but no very fastidious or extremely oxygen-sensitive organisms were recovered. This suggests that such organisms may not play a significant role in causing clinical infections.


Fermentation kinetics and continuous process of L-asparaginase production. For the purpose of obtaining L-asparaginase in quantities from Erwinia aroideae, cell growth and enzyme formation were investigated in both batch and continuous fermentation. Using yeast extract as a growth-limiting substrate, the relationship between specific growth rate and substrate concentration was found to fit the Monod equation. The optimum temperature for enzyme production was 24 C, although cell growth was higher at 28 C. The enzyme yield reached its maximum of 4 IU/ml during the negative acceleration growth phase which occurs just prior to stationary growth. Compared to batch fermentations, the continuous fermentation process gave a lower enzyme yield except when the fermentation was conducted at a dilution rate of 0.1 hr(-1). The graphical method frequently used for prediction of continuous fermentation does not apply to L-asparaginase production by E. aroideae. The optimum temperature for enzyme production in continuous process was 24 C, which was the same as in batch process. Increasing the temperature from 24 to 28 C resulted in a 20% loss of enzyme yield.


An experiment to challenge the resistance to tuberculosis in B.C.G. vaccinated cattle in Malawi.


Control of bovine foot rot by treatment of feedlot litter with paraformaldehyde.


Salmonella cultures typed during the years 1950-1971 for the service laboratories of the New York State Veterinary College at Cornell University.


Clinical observations on reproduction in a pony stud.


Modern concepts of neonatal disease in foals.


Implications of the effects of maternal diets in various species.


Influence of maternal protein and energy nutrition during gestation on progeny performance in swine.


Influence of energy intake by the neonatal pig on subsequent growth and development.


A technique for direct bonding of orthodontic attachments.


Gastric emptying and secretion in the milk-fed calf. 1. For a few weeks, immediately post-natally, the abomasum of the ruminant stomach can be regarded as the analogue of the simple stomach for at this time food passes directly to the abomasum because of closure of the oesophageal groove.2. Using standard fractional and serial test meal techniques discussed by Hunt (1956) and adapted for use in the calf, abomasal emptying, acid and pepsin secretion have been examined. Phenol red was used as a marker to measure volume changes of the test meal.3. Abomasal emptying is exponential in character whether large or small volumes of fluid are instilled into the abomasum. The initial and end phase of emptying shows variable rates between animals.4. Glucose and lactose solutions inhibit abomasal emptying as well as acid production.5. Sodium chloride and sodium bicarbonate of low concentration, near isotonic with blood plasma, stimulate abomasal emptying but the bicarbonate is most effective. Hypertonic solutions of these salts inhibit abomasal emptying.6. Pepsin secretion in the abomasum of the calf is not affected by test meals of glucose, lactose, sodium chloride or sodium bicarbonate.7. These results shows a great similarity between the physiology of the abomasum of the milk-fed calf and the simple stomach. This suggests that the same duodenal receptors, discussed by Hunt & Knox (1968), which control gastric movement in man are also effective in controlling gastric emptying in the milk-fed calf.


The effect of some molecules and ions on gastric function in the milk-fed calf. 1. The abomasum of the milk-fed calf has been examined using an adaptation of the Serial Test Meal method devised by Hunt & Spurrell (1951). The emptying process, acid secretion and pepsin secretion were studied.2. Using serial test meals of simple solutions instilled into the abomasum via a cannula, our investigation leaves no doubt that the osmolarity of the abomasal contents significantly modifies the rate of abomasal emptying.3. Hypotonic and isotonic solutions of sodium chloride and sodium bicarbonate increase abomasal emptying but bicarbonate is most effective.4. Increasing the concentration of solutes in the abomasal contents slows abomasal emptying. Sodium chloride, sodium bicarbonate, ammonium chloride and urea do not delay abomasal emptying until hypertonic concentrations are attained. Hypotonic solutions of potassium chloride, calcium chloride, glucose, lactose, hydrochloric acid and acetic acid delay abomasal emptying.5. The results obtained in the calf show that the abomasum is under restraint probably from duodenal receptors as is the simple stomach (Hunt & Knox, 1968) and that an osmoreceptor as postulated by Hunt (1956) is an important factor in this mechanism.6. Acid secretion is inhibited when hypertonic solutions are instilled into the abomasum.7. Pepsin secretion is not affected by simple solutions in the abomasum.8. Gastric function in the milk-fed calf appears to be controlled by mechanisms essentially similar to those already demonstrated in the simple stomach.


Dental health forms.

Dental health forms.


Self-made milled attachments.

Self-made milled attachments.


Difficulties of subgingival impression taking with elastomeric meterials. II.


Copper ring impressions for partial dentures.


Radiation-sterilized split skin: a new type of biological wound dressing. A preliminary report.


Local radiation injury and its surgical treatment. Report of 170 cases.


Repair of skin defects in the Achilles tendon region. Case report.


The basement membrane in normal skin and split skin autografts in man. An electron microscopic study.


12 years of free skin vestibuloplasty.

12 years of free skin vestibuloplasty.


Transoral rib grafting for mandibular alveolar atrophy--a progress report.


Use of a biocompatible interface for combining tissues and prostheses in oral surgery.


The use of marrow-cancellous grafts in the regeneration of mandibular bone.


Decalcified bone matrix implantation in bone defects.


The effect of acridine orange on the structure of ribosomes.


Visuo-sensory development of the child.

Visuo-sensory development of the child.


Metabolism of ( 14 C)-serotonin in the caudate nucleus, hypothalamus and reticular formation of the rat after ethanol administration.


Spectroscopic properties of oligonucleotides excised from the anticodon region of phenylalanine tRNA from yeast.


Immunofluorescent demonstration of toxoplasma antigens within the skin of mice in the early stage of percutaneous infection.


Temperature dependence of CD spectra of DNA from various sources.


Hypertrophic obstructive cardiomyopathy of the left ventricle (idiopathic hypertrophic subaortic stenosis).


Infectious myocarditis.

Infectious myocarditis.


Alcoholic cardiomyopathy.

Alcoholic cardiomyopathy.


Toxic agents and cardiomyopathies.

Toxic agents and cardiomyopathies.


Cardiomyopathy associated with endocrine disorders.


Cardiomyopathies of the senescent and senile.


The avoidance of surgical complications in podiatry.


The role of radical surgery in the treatment of recurrent nasal polyposis.


Medicaid utilization in Georgia hospitals for fiscal 1971.


Radioimmunoassay: current status and clinical application.


Immunodepressive effect of Rauscher leukemia virus on antinuclear antibody formation in New Zealand black mice.


Insect transfer of labeled erythrocytes, with implications for circulating tumor cells.


Suppression of murine tumor growth by immune reaction to the Bacillus Calmette-Guérin strain of Mycobacterium bovis.


Tumor control and pulmonary metastasis in fractionally irradiated transplanted mammary carcinoma in C3H mice.


Alteration of skin in Gross leukemia. 3. Quantitation of the role of tumor cells.


Pancreatic changes in maturity onset diabetes mellitus.


Platelet lipids and coagulation in plasma. II. Thromboplastic activity.


Analysis of a clinical evaluation of materials used intraorally.


Maxillary complete denture posterior palatal seal: variations in size, shape, and location.


Preparation design and margin distortion in porcelain-fused-to-metal restorations.


The complete-coverage restoration and the gingival sulcus.


Use of polyethylene in cranial implants.


A mouthstick for quadriplegic patients.

A mouthstick for quadriplegic patients.


Theoretical models of cellular dielectrophoresis.


Household wringer injuries: a three-year review.


Small animal bites: the role of primary closure.


A comparative study of synthetic and biological materials for wound dressings.


The role of bradykinin in canine gram-negative bacteremia.


Laser excision of acute third-degree burns followed by immediate autograft replacement: an experimental study in the pig.


On the origin of the hypertrophic scar.

On the origin of the hypertrophic scar.


Vasectomy matters.

Vasectomy matters.


José de Acosta.

José de Acosta.


Heroin maintenance for heroin addicts: issues and evidence.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 13-1973.


Evidence of vasoconstrictor sympathetic nerves in brain vessels of mice.


A technique for microelectrophoretic study of single neurones in the awake non-paralyzed cat.


Fatal acute meningoencephalitis due to lymphocytic choriomeningitis virus.


Induction of pyrimidine dimers in bacterial DNA by 365 nm radiation.


Metabolic differences between hepatitis and cholestasis in human liver.


Microsomal biotransformation system in cholestasis.


Physiologic and pathophysiologic aspects of the hepatic hemodynamics.


Radionuclides in the investigation of liver disease.


Some aspects of circulatory disturbances in cirrhosis of the liver.


New surgical approaches to portal hypertension.


Hepatic coma: present concepts of pathogenesis and therapy.


Regulatory effect of amino acids on polyribosomes and protein synthesis of liver.


The treatment of acute liver failure.

The treatment of acute liver failure.


The nature of Australia antigen: infectious and genetic characteristics.


Immunopathology of liver disease.

Immunopathology of liver disease.


Treatment of chronic hepatitis.

Treatment of chronic hepatitis.


Liver transplantation: a clinical and immunological appraisal.


Encephalopathy and fatty liver (Reye's syndrome).


Evolution of the hepatic lesion in Wilson's disease (hepatolenticular degeneration).


Alcoholic hepatitis: its therapy and prognosis.


Tetracycline and the liver.

Tetracycline and the liver.


Hepatic drug reactions.

Hepatic drug reactions.


Bile salts and the liver.

Bile salts and the liver.


Mabel Blake Cohen. 1908-1972.

Mabel Blake Cohen. 1908-1972.


In memoriam: William Caudill, Ph.D.

In memoriam: William Caudill, Ph.D.


Beckett, Sarraute, and the perceptual experience of schizophrenia.


Seneca--a new approach to his personality.


The role of bronchodilators in bronchospastic disease. A clinical and laboratory study.


Bacterial flora of the stools of gastroenteritis cases among Filipino adults.


Sensitivity tests of Enterobacteriaceae in diarrhoeal diseases in Djakarta.


Mechanism of plasmodial drug resistance.


Serum vitamin B 12  level and vitamin B 12  absorption in patients with Plasmodium falciparum malaria.


Experimental infection of Plasmodium falciparum by Anopheles vagus in South Vietnam.


Transcapillary transport of D- and L-glucose in isolated skeletal muscle.


The identification of an organism isolated from maned sheep as Mycoplasma mycoides subsp. mycoides.


Isolation and characterization of a new porcine mycoplasma.


Acoustic trauma and cochlear microcirculation. An experimental and clinical study on pathogenesis and treatment of inner ear lesions after acute noise exposure.


Analytical variability in the single disk antimicrobial susceptibility test.


Evaluation of a selective medium for mycobacteria.


Compact colony-forming activity and the effect of pH on compact-type growth of Staphylococcus aureus strains in serum-soft agar.


Evaluation of a simple preservative for bacteriological tests on urine. A urine preservative containing NaCl and polyvinyl-pyrrolidone has been in general use at this hospital for three years. It enables the specimen to be held at room temperature for 48 hours or more without appreciable change in the content of viable bacteria. The results of bacteriological tests performed on 2452 preserved urine specimens are compared with those from a control series examined before the method was introduced. A marked improvement in accuracy is demonstrated. The preservative can be very easily and cheaply prepared in any laboratory.


Immunodepressive activity of cycloleucine (NSC-1026) in mice.


Pharmacologic studies in man with crystallized L-asparaginase (NSC-109229).


Further clinical trials with porfiromycin (NSC-56410) (large intermittent doses).


Treatment of Ewing's sarcoma with adriamycin (NSC-123127).


Regression of intradermal malignant melanoma after intralesional injection of Mycobacterium bovis strain BCG.


Combination therapy with 5-fluorouracil (5-FU; NSC-19893) and 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU; NSC-409962) for disseminated gastrointestinal carcinoma.


Clinical experience with 5-(3,3-bis(2-chloroethyl)-1-triazeno)imidazole-4-carboxamide (NSC-82196) in the treatment of metastatic malignant melanoma.


5-(3,3-dimethyl-1-triazeno)imidazole-4-carboxamide (NSC-45388) in the treatment of lymphoma.


On the structure of yeast tRNA Phe . Complementary-oligonucleotide binding studies.


The presence of an exonuclease in highly purified DNA polymerase from bakers' yeast.


Isolation of a glucose-binding lipoprotein from yeast plasma membrane.


Complexes of aminoacyl-tRNA synthetases with tRNAs as studies by partial nuclease digestion.


The primary structure of the major parvalbumin from hake muscle. Overlapping peptides obtained with chemical and enzymatic methods. The complete amino-acid sequence.


Antineoplastic and immunosuppressive drugs. 3. Adverse effects and therapeutic problems.


Analysis of bacteriophage-M 13-DNA replication in an Escherichia coli mutant thermosensitive in DNA polymerase 3.


A new class of heptose-defective mutant of Salmonella typhimurium.


Size distribution of circular DNA from petite-mutant yeast lacking rho DNA.


Studies on energy-linked reactions. Isolation and characterisation of oligomycin-resistant mutants of Saccharomyces cerevisiae.


Evidence for a common transport system for cytosine, adenine and hypoxanthine in Saccharomyces cerevisiae and Candida albicans.


Studies on energy-linked reactions. Genetic analysis of oligomycin-resistant mutants of Saccharomyces cerevisiae.


Recycling of initiation factors IF-1, IF-2 and IF-3.


Insulin and glucagon release from monolayer cell cultures of pancreas from newborn rats.


Medical management of peptic ulcer with reference to anti-ulcer agents in other gastro-intestinal diseases.


The effect of acid, pepsin and trypsin on human colostral IgA agglutinins.


Study of the specificity of amino acid binding protein from Saccharomyces cerevisiae membranes.


Microbial biomass production of solid hydrocarbons.


Cell division of penicillin-induced filaments of Escherichia coli. Sequence of septum formation and role of protein synthesis in reversion.


Glucose repression of the synthesis of some colicins.


Antimicrobial agents. XVI. Adaptation of dermatophytes to antimycotics.


Uses of the goniometer in electron microscopy of ultrathin sections.


A simple and effective technique for haversian canals injection.


Real-time computing in patient monitoring.


Application of the plastic linear marking method to mammalian embryos.


Obituary. Dr. M. Josephine Mackerras.

Obituary. Dr. M. Josephine Mackerras.


Social research problems in studies involving multiphasic health testing.


Phase-contrast photomicrography of isolated rat liver mitochondria.


A host-parasite list of the protozoan and helminth parasites of New Guinea animals.


Challenges--then and now.

Challenges--then and now.


Mechanisms of eccrine anidrosis. 3. Scanning electron microscopic study of poral occlusion.


Isolation and characterization of polyribosomes from the epidermis of the newborn rat.


The histiotypic organization of the hypertrophic scar in humans.


A clinical trial of the effects of oral beta-carotene on the responses of human skin to solar radiation.


Kidney transplantation in Iowa.

Kidney transplantation in Iowa.


Why a union?

Why a union?


Organ procurement and preservation.

Organ procurement and preservation.


Hypodontia in the permanent dentition.

Hypodontia in the permanent dentition.


A cephalometric study for preschool children.


Medicare: concepts and misconceptions.

Medicare: concepts and misconceptions.


Evaluation of a head positioner for panoramic radiography.


Selection of amalgam alloys: particle form, new formulas.


The preparation of cholinergic synaptosomes from bovine superior cervical ganglia.


Characteristics and products of a cell-free polypeptide synthesizing system from neonatal and adult mouse brain.


Role of transfer factor in treating complications of smallpox vaccination.


A comparison by electron microscopy of Plasmodium berghei freed by ammonium chloride lysis to P. berghei freed by ultrasound in a continuous-flow system.


Parasitology and the United States Public Health Service: a relation of a science and government.


Disintegration of tablets.

Disintegration of tablets.


Toxogonin and pralidoxime: kinetic comparison after intravenous administration to man.


Effects of nylidrin in endotoxin shock.

Effects of nylidrin in endotoxin shock.


An electron microscopic observation of erythrocytic cycle of mammalian malaria (Plasmodium berghei yoeli).


Early events of rabies virus replicaton in tissue cultures. An electron microscopic study.


Severe acute radiation injuries of the hands. Report of two cases.


Pedicle patch urethroplasty for cure of urethral stricture.


Subjective experiences during a 32-year period after resurfacing of hands for severe and acute radiation burns.


The zigzag pectoro-subaxillary flap.

The zigzag pectoro-subaxillary flap.


Treatment of eyelid deformities due to burns.


Surgical correction of midfacial retrusion (nasomaxillary hypoplasia) in the presence of normal dental occlusion.


Cervicofacial actinomycosis.

Cervicofacial actinomycosis.


Successful replantation of a large severed ear fragment.


A history of coronary artery surgery.

A history of coronary artery surgery.


Use of adhesives for reimplantation and autografting of the renal pole.


Hyperventilation and cerebral blood flow.


Medical education in Alaska--undergraduate and postgraduate.


Use of a desk-top calculator to interpret acid-base data.


Microinvasive carcinoma of the cervix.

Microinvasive carcinoma of the cervix.


The role of the placenta in fetal and perinatal pathology. Highlights of an eight months' study.


Quantitation of factors affecting the alveolar-arterial P O2  difference in thoracotomy.


The effects of tidal-volume change with positive end-expiratory pressure in pulmonary edema.


Vitrectomy.

Vitrectomy.


Iris suture in rabbits.

Iris suture in rabbits.


The selective excision of arylalkylated products from the DNA of Escherichia coli treated with the carcinogen 7-bromomethylbenz(a)anthracene.


New species of tyrosine tRNA in nonsense suppressor strain of yeast.


The inhibition in vitro of bacterial DNA polymerases and RNA polymerase by antimalarial 8-aminoquinolines and by chloroquine.


Ribonuclease properties and activity in the presence of dimethylsulfoxide.


The effect of surgical glove powder in the anterior chamber of rabbit eyes.


Power analysis of the human electroretinogram.


Early development of the human retina.

Early development of the human retina.


Propranolol-induced insulin release in isolated rat islets of Langerhans.


Effects of trimethoprim and its antagonists on RNA synthesis in Escherichia coli.


The dynamic state of the lymphocyte membrane. Factors affecting the distribution and turnover of surface immunoglobulins.


Helper activity of histocompatibility antigens on cell-mediated immunity.


Metabolic characteristics of lymphocyte surface immunoglobulins.


Antibody-induced redistribution of HL-A antigens at the cell surface.


Antigenic competition in vitro between heterologous erythrocytes.


Responses of athymic ("nude") mice to sheep red blood cells.


Lymphocyte typing in dogs. II. Production and storage of antisera.


Stimulation of anti-hapten response by heterologous protein devoid of hapten.


Ventricular fibrillation from low calcium during liver transplantation in the pig.


Luigi Rolando, 1773-1831.

Luigi Rolando, 1773-1831.


Anticoagulation in microvascular surgery.


Mutator gene studies in Escherichia coli: the mutS gene. We report here on a study of a mutator gene (mutS) that causes transition mutations in Escherichia coli. We have used the trpA system to show that A:T-->G:C and G:C-->A:T transitions occur. Not all A:T pairs are equally susceptible to mutS action however, since the A:T pair at the trpA223 site reverts at a frequency similar to, if not identical with, the frequency in a mut(+) background. Presumably this is a consequence of neighboring bases, because other A:T pairs are reverted by mutS in the same gene; and an A:T pair in the lac operon is reverted at two widely separated points on the chromosome, and in two orientations relative to the trp sense strand. In addition, we have shown that the mutS1 allele is recessive to wild type, and trans active.


The characteristics and genetic map location of a temperature sensitive DNA mutant of E. coli K12. A temperature sensitive strain of E. coli K12 has been isolated in which residual DNA synthesis occurs at the 40 degrees C restrictive temperature; syntheses of RNA, protein and DNA precursors are not directly affected. The mutation has been designated dna-325 and is located at 89 min on the E. coli map in the same region where the dnaC locus is found. dnaC mutants are considered to be defective in DNA initiation. Some of the data are consistent with the view that the dna-325 mutation is temperature sensitive in the process of DNA initiation rather than DNA chain elongation: (1) more than two cell divisions occur after a shift to 40 degrees C; (2) upon a shift down to 30 degrees C, cell division occurs again only after the DNA content of the cells has doubled; (3) 80% more DNA is made at 30 degrees C in the presence of chloramphenicol after prior inhibition of DNA synthesis at 40 degrees C. These three observations indicate that rounds of DNA replication were completed at 40 degrees C. Also (4) infective lambda particles can be made at 40 degrees C long after bacterial DNA replication has ceased. It appears however that some DNA initiation can occur at 40 degrees C since (1) a limited amount of DNA synthesis does occur at 40 degrees C after prior alignment of the chromosomes by amino acid starvation at 30 degrees C, and (2) after incubation in bromouracil at the restrictive temperature, heavy DNA is found with both strands containing bromouracil.


Computerized meiotic mapping in Aspergillus nidulans. A computer system for the storage and processing of microbial meiotic data has been developed. Based on the language Fortran 4, the program retrieves relevant data and determines the order, map distances, and coefficient of coincidence for any three-gene group. Meiotic data from Aspergillus nidulans were used to test the program. A total of 61 three-gene sequences were processed, and the results were found to be compatible with the published values. The advantages and disadvantages of computer analysis for genetic analysis are discussed.


The inability of yeast transfer RNA to suppress an amber mutation in an E. coli system. Transfer RNA from super-suppressor mutants of Saccharomyces cerevisiae cannot suppress an amber mutation in vitro in an E. coli protein synthesizing system. It is tentatively concluded that the yeast amber suppressor does not contain a transfer RNA altered in the anticodon.


Studies of macromolecular structure by  13 C nuclear magnetic resonance. II. A specific labeling approach to the study of histidine residues in proteins.


The vegetarian diet. Scientific and practical considerations.


Pathology and epidemiology of atherosclerosis.


Caries-inhibiting action of three different topically-applied agents on incipient lesions in newly erupted teeth: results after 24 months.


Essential hypertension: A childhood disease?


The past, present, and future status of oral surgery in the United States.


Microbiological reduction and resolution of prostaglandins. Synthesis of natural PGF 2   and ent-PGF 2   methyl esters.


Root surface caries: review of the literature and significance of the problem.


Bacterial antigens and antibodies in human periodontal tissue.


Point of view.

Point of view.


Periodontal status of renal transplant patients receiving immunosuppressive therapy.


Gingivitis in hypogammaglobulinemia.

Gingivitis in hypogammaglobulinemia.


Neurological manifestations in patients after renal transplantation.


Combined radiographic, manometric, and histologic localization of the canine lower esophageal sphincter.


Functional, metabolic and structural studies of the chronic canine cardiac autotransplant.


On the phylogeny of t-RNA's.

On the phylogeny of t-RNA's.


The dynamics of cellular differentiation and associated pattern formation.


The turnover of proteins and the usage of information.


A mode of treatment for incarceration of the penis.


Sir Reginald Watson-Jones. 1902-1972.

Sir Reginald Watson-Jones. 1902-1972.


Knee disarticulation as an amputation level.


Factors influencing competence of Escherichia coli for lambda-phage deoxyribonucleic acid infection.


Cell-cooperation in anti-sheep red blood cell antibody response in mouse spleen cell cultures. Use of antilymphocyte globulin for selective suppression of the antigen reactive cells.


Studies on bacteriophage distribution. II. Isolation and host rage based classification of phages active on three species of Enterobacteriaceae.


Application of experimental keratoconjunctivitis shigellosa in chemotherapeutic evaluation of rifampicin to bacillary dysentery.


Copernicus remembered.

Copernicus remembered.


Clinical trial of dipotassium chlorazepate, a new benzodiazepine derivative.


Clinical evaluation of chlorazepate, a new ataractic.


ADN programs accredited by the National League for Nursing--1973, Department of Associate Degree Programs.


Myelomeningocele.

Myelomeningocele.


Clinical experience with hypothalamic releasing hormones. 2. Luteinizing hormone-releasing hormone and other hypophysiotropic releasing hormones.


Prostaglandins in luteal function.

Prostaglandins in luteal function.


Recent studies on functions and control of prolactin secretion in rats.


Studies on prolactin in man.

Studies on prolactin in man.


Biological applications of electron ionization and chemical ionization mass spectrometry.


Gender dimorphic behavior and fetal sex hormones.


Techniques for assessing the effects of sex hormones on affect, arousal, and aggression in humans.


The experimental development of modern resuscitation.


An experimental evaluation of the thermodilution method for determination of cardiac output and of intracardiac right-to-left shunts.


The visualization of genes in action.

The visualization of genes in action.


Angiographic contrast media: a historical résumé.


Assembly of bacterial ribosomes.

Assembly of bacterial ribosomes. I have not mentioned the remarkable progress made mainly by Fellner and his co-workers (86) in the elucidation of the primary structure of rRNA's and by Wittmann and his co-workers (87) in determining the structure of several ribosomal proteins. Such knowledge of primary structures is certainly the basis of complete understanding of the structure of the ribosome. With the current progress in technology, complete elucidation of the primary structure of all the ribosomal components is probably a matter of time. As indicated in this article, a rough approximation of the three-dimensional structure of ribosomes is likely to emerge soon. Although not mentioned in this article, studies of ribosomes from higher organisms are also progressing. We must, therefore, consider what further studies should be conducted and what kinds of questions we would like to solve. Some groups of investigators aim to elucidate the complete three-dimensional structure of ribosomes and to find out how these complex cell organelles function; they hope to determine the conformational changes of many of the component molecules within the ribosome structure in response to external macromolecules and cofactors engaged in protein synthesis. Such knowledge will also be important in enabling us to understand the regulation of translation of genetic messages. Other groups of investigators aim to elucidate the complex series of events which originate in the transcription of the more than 60 genes and culminate in the formation of the specific structure of the organelle. Complete reproduction in vitro of all the assembly events that occur in vivo should not be difficult to achieve in principle. It should then become possible to study in vitro any factor regulating the biogenesis of the organelle. Although we do not know whether such studies would reveal any new fundamental principle that governs the complex circuits of interconnected macromolecular interactions, the achievement of such a complete in vitro system would represent a necessary step in the comprehensive understanding of biogenesis of organelles, and eventually, of the more complex behavior and genesis of cells (89).


Cannabis and alcohol: effects on stimulated car driving. The effects of cannabis and alcohol on simulated car driving were studied. Cannabis resin containing 4 percent Delta(1)-tetrahydrocannabinol was administered orally in three doses equivalent to 8, 12, and 16 milligrams of that component. Alcohol was given orally in one standard dose of 70 grams. Both cannabis and alcohol increased the time required to brake and start, whereas alcohol increased while cannabis decreased the number of gear changes. An effect of dosage on response was observed with cannabis.


Enhancement of renal preservation by furosemide.


Multiple modes of action of cyclophosphamide on plasmodial infections in rats.


Malaria in the Pare area of Tanzania. IV. Malaria in the human population 11 years after the suspension of residual insecticide spraying, with special reference to the serological findings.


Development of T. rangeli metacyclic forms from triatomid bugs' salivary glands ingested by Rhodnius ecuadoriensis.


The effect of tetracycline on Plasmodium falciparum in the Gambia.


Mutations rendering Salmonella typhimurium resistant to 3-aminotriazole in the presence of histidine.


Mutants of Salmonella typhimurium resistant to triazole.


Alternative mechanisms of apparent supernormal atrioventricular conduction.


Occupational therapy and intern collaboration. I.


Pulmonary arterial stenosis with wide splitting of the second heart sound due to mediastinal fibrosis.


Objective evaluation of projective material.


Schools of Public Health in the USA and Canada.


Threonine-sensitive aspartokinase--homoserine dehydrognease of Escherichia coli K 12 . Evidence for a cooperative tetramer.


Threonine-sensitive aspartokinase--homoserine dehydrogenase complex from Escherichia coli. Subunit stoichiometry and size of the catalytic unit.


Haloacetol phosphates. A comparative study of the active sites of yeast and muscle triose phosphate isomerase.


Kinetic studies on solid-supported  -galactosidase.


Susceptibility of paramyosin to proteolysis and the relationship to regions of different stability.


Prebiotic phosphorylation-nucleotide synthesis with apatite.


Location of sulfhydryl and disulfide groups in bovine  -lactoglobulins and effects of urea.


Enzymatic synthesis of deoxypseudouridylic acid and a study of certain of its properties.


The DNA of the thoracic muscle of Manducta sexta.


Ultraviolet light-induced stable complexes of DNA and DNA polymerase.


Size heterogeneity of the large ribosomal subunits and conservation of the small subunits in eucaryote evolution.


Transcription of chromatin from two classes of human leukemic leukocytes.


Studies on release of N-acetylphenylalanyl-tRNA from its ribosomal complex: effects of monovalent cations and antibiotics.


Two cytoplasmically inherited chloramphenicol resistance loci in yeast (Saccharomyces cerevisiae).


Ethidium bromide resistance and petite induction in Saccharomyces cerevisiae.


Radiotherapeutic management of small intestinal lymphoma with malabsorption.


Malignant lymphoma of the small intestine in Iraq. (Middle East lymphoma).


Malignant lymphoma including Hodgkin's disease occurring in the vicinity of a large medical center (Olmsted County, Minn., 1945 through 1969).


Therapy with carbenicillin and gentamicin for patients with cancer and severe infections caused by gram-negative rods.


Clinical trials of combination chemotherapy with BCNU and vincristine in the treatment of malignant melanoma in Uganda.


Classification of benign and malignant breast tumors on the basis of 36 radiographic properties.


The throat culture: its techniques, pitfalls, limitations and meaning.


Study of Fab and F(ab') 2  from rabbit IgG for capacity to induce lymphocyte-mediated target cell destruction in vitro.


Experimental manipulation of desmosome formation. In the corneal epithelium of the embryonic chick there is a 3- to 4-fold increase in desmosomes between the 15th and 16th days of incubation which has not been noted in earlier studies of this tissue. This finding has made it feasible to study the effects of the local cell environment on desmosome formation. Cells of 15-day corneas which were forming desmosomes rapidly, were dispersed and combined in culture with cells from 10-day corneas which were forming few desmosomes. Surfaces of the same 15-day cell which were confronted with either another 15-day cell or a 10-day cell were compared. Desmosomes formed preferentially on the surface adjacent to a like cell. When 15-day cells were confronted with pigment cells, desmosomes formed almost exclusively on the surface adjacent to a like cell. Evidence for such localized differences on the same cell surface emphasize the importance of the immediate cell environment in desmosome formation. The observation that single desmosome plaques form occasionally on lateral cell surfaces has been noted previously. This finding was confirmed.


A morphometric study of the removal of phenobarbital-induced membranes from hepatocytes after cessation of threatment. It is well known that phenobarbital (PB) treatment produces an increase in the amount of cytoplasmic membranes of hepatocytes, with a parallel enhancement in the activity of drug-metabolizing enzymes. However, little is known about how the induced membranes are removed after the drug treatment is stopped. To consider this problem, the recovery of rat hepatocytes from PB induction (five daily injections, 100 mg/kg) was followed morphometrically. Treatment with PB produced a cellular enlargement (26%) due to increases in the volume of the cytoplasmic matrix (20%) and the volume (100%) and surface area (90%) of the smooth-surfaced endoplasmic reticulum (SER). The volume of the nuclei and the surface area of the Golgi apparatus were also increased, but no changes were detected in the volumes of the mitochondria or peroxisomes. The SER membranes induced by the PB were removed within 5 days after the end of the treatment period. During this period of membrane removal, we observed an increase in the volume (800%) and number (96%) of autophagic vacuoles without a change in dense bodies. A morphometric analysis of the content of the autophagic vacuoles showed that the endoplasmic reticulum membranes were preferentially removed, and from this we conclude that the formation of autophagic vacuoles was not a random process. Our findings show that the removal of excess cytoplasmic membranes is associated with an increase in autophagic activity and thus demonstrates the presence of a specific cellular mechanism which may be responsible for the bulk removal of PB-induced membranes.


Cell contacts in the mouse mammary gland. I. Normal gland in postnatal development and the secretory cycle. The nature and distribution of cell contacts have been examined in thin sections and freeze-fracture replicas of mammary gland samples from female C3H/Crgl mice at stages from birth through pregnancy, lactation, and postweaning involution. Epithelial cells of major mammary ducts at all stages examined are linked at their luminal borders by junctional complexes consisting of tight junctions, variable intermediate junctions, occasional small gap junctions, and one or more series of desmosomes. Scattered desmosomes and gap junctions link ductal epithelial and myoepithelial cells in all combinations; hemidesmosomes attach myoepithelial cells to the basal lamina. Freeze-fracture replicas confirm the erratic distribution of gap junctions and reveal a loose, irregular network of ridges comprising the continuous tight-junctional belts. Alveoli develop early in gestation and initially resemble ducts. Later, as alveoli and small ducts become actively secretory, they lose all desmosomes and most intermediate junctions, whereas tight and gap junctions persist, The tight-junctional network becomes compact and orderly, its undulating ridges oriented predominantly parallel to the luminal surface. It is suggested that these changes in junctional morphology, occurring in secretory cells around parturition, may be related to the greatly enhanced rate of movement of milk precursors and products through the lactating epithelium, or to the profound and recurrent changes in shape of secretory cells that occur in relation to myoepithelial cell contraction, or to both.


The role of microfilaments in frog skin ion transport.


Culture of erythropoietic cells from chick blastoderms.


Fractographic studies of plastic-embedded cells by scanning electron microscopy.


Problem-solving case presentations in clinical fixed prosthodontic instruction program.


Development and evaluation of standardized measure for assessing diagnostic skills.


Programming for sharp curets and sickle scalers.


Marijuana.

Marijuana.


Aggressive reactions to frustration in urban Guatemalan children: the effects of sex and social class.


The Napolenic itch.

The Napolenic itch.


Hypothalamic cooling, heat production and plasma lipids in the ox.


Criteria for the selection of patients for treatment with tolbutamide.


Tolbutamide in chemical diabetes mellitus in children.


Vertebral angiography in the diagnosis of intraspinal masses in upper cervical region.


Pseudomonas osteochondritis of sternocostal junction.


David Nieto, M.D. (1654-1728). Rabbi, physician, philosopher, and man of culture.


Scurvy in the Talmud.

Scurvy in the Talmud.


Rev. Thomas Thacher 1620 to 1678. First colonial medical pamphleteer.


Changes in plasma estriol and progesterone during labor induced with prostaglandin F2alpha or oxytocin.


Primary non-traumatic spontaneous cerebro-spinal fluid rhinorrhea with normal cerebro-spinal fluid pressure.


Neurophysiological tests for stereotactic identification of rhinencephalic "targets".


Temporal epilepsy, c.q. psychomotor epilepsy. Experiences and present-day conceptions.


Historical aspects and changing trends in psychoanalytic therapy.


The state of tyrosine in inorganic pyrophosphatase of baker's yeast.


Transport of spermatozoa in the female reproductive tract.


Allport, Freud, and the clean little boy.


Fetography.

Fetography.


Clinical anatomy of the obliterated umbilical vein.


Pancreatic transplantation without duodenum in the dog.


Alkali hydrolysis of RNA of Escherichia coli deposited on filter paper disks.


Pancreatic duct to ureter anastomosis for exocrine drainage in pancreatic transplantation.


A scheme for the preliminary fractionation of E. coli extracts for the large-scale isolation of tryptophanyl- and lysyl-tRNA-synthetases and tRNA.


A simple preparative polyacrylamide disc gel electrophoresis apparatus: purification of three branched-chain amino acid binding proteins from Escherichia coli.


Single-step separation of RNases I, II, and 3 present in extract of Salmonella typhimurium.


An assay for transaminase B enzyme activity in Escherichia coli K-12.


Continuous positive pressure breathing in chronic obstructive lung disease: effect on minute ventilation and blood gases.


Deposition of inhaled aerosols.

Deposition of inhaled aerosols.


Combination chemotherapy and survival in advanced Hodgkin's disease.


Chemotherapy of lymphoma with mechlorethamine and vinblastine.


Management of patients with non-Hodgkin's lymphoma.


Anthranilate synthetase. In vitro complementation between Serratia marcescens and Escherichia coli subunits.


Factors affecting scale-up of affinity chromatography of  -galactosidase.


A controlled trial of the effects of bromhexine on the symptoms of out-patients with chronic bronchitis. A report to the Research Committee of the British Thoracic and Tuberculosis Association.


Closing volume--a test of small airway function?


Golden anniversary of bronchography by Sicard and Forestier.


Cytology in diagnosis of cancer affecting the lung. Results in 1,000 consecutive patients.


Epinephrine-induced changes in the pulmonary pressure-volume curve of the intact and hypovolemic rabbit.


An analysis of survey data obtained by a service agency for older people.


Avicenna and the care of the aged.

Avicenna and the care of the aged.


Seymour L. Lustman (1920-1971).

Seymour L. Lustman (1920-1971).


A special report: the evolution of geriatrics in Austria.


Lothair Henry Rubinstein (1908-1971).

Lothair Henry Rubinstein (1908-1971).


Moshe Woolf (1878-1971).

Moshe Woolf (1878-1971).


Hilda Abraham (1906-1971).

Hilda Abraham (1906-1971).


'The voice of the intellect is a soft one'. A review of the writings of Anna Freud, volume IV, 1945-1956.


Temperature-sensitive relaxed Phenotype in a stringent strain of Escherichia coli. A temperature-sensitive elongation factor G (EF-G) mutation carried by a rel strain of Escherichia coli was transferred to a rel(+) strain. The recombinant (AA-10) was found to be temperature-sensitive "relaxed" for ribonucleic acid (RNA) regulation. However, when a temperature-sensitive EF-G is present in a strain temperature-sensitive for valyl-transfer RNA (tRNA) synthetase, this new strain (AA-16) is no longer temperature-sensitive "relaxed" for RNA regulation. In strain AA-10, all tRNAs remain fully charged, and in AA-16 tRNA(val) becomes completely discharged at the nonpermissive growth temperature. Also, synthesis of ppGpp is not observed in strain AA-10 at 42 C but is observed at 42 C in strain AA-16.


Effect of alanine, leucine and fructose on lysyl-transfer ribonucleic acid ligase activity in a mutant of Escherichia coli K-12. A mutant of Escherichia coli K-12 was examined which has growth medium-dependent lysyl-transfer ribonucleic acid (tRNA) ligase activity. In minimal medium or 0.5% yeast extract, the activity of the enzyme in the mutant strain was 5 to 10% of wild type. However, when the mutant was grown in a highly enriched medium, such as AC broth (Difco), the activity of the mutant ligase increased 10- to 20-fold. We found that the supplementation of 0.5% yeast extract by l-alanine plus d-fructose replaces the need for the highly enriched medium. Fructose plus l-leucine and fructose plus l-alpha-amino-n-butyric acid were also stimulatory, but not as effective as fructose and alanine. With minimal medium, a combination of carbohydrate (fructose or glucose) plus alanine and leucine was required to replace the enriched medium. The most effective combination was fructose, glucose, alanine, and leucine. Lysyl-tRNA ligase was stimulated 1.5 to 2-fold in the wild-type strain or Hfr H (Hayes) by fructose plus alanine when these strains were cultured in 0.5% yeast extract. Experiments employing the combined technique of density labeling with D(2)O and isopycnic centrifugation in cesium chloride indicated that the increased activity of lysyl-tRNA ligase observed in AC broth or in the presence of fructose, glucose, alanine, and leucine is due to the synthesis of new enzyme.


Plasmid-associated functions of a stable Flac. Several functions associated with the stable plasmid, FlacS, have been examined. Our results indicate that the sex pili synthesized by Escherichia coli strains carrying FlacS are altered in some manner as evidenced by a very inefficient adsorption of male-specific phages. On the other hand, FlacS-mediated entry exclusion of related plasmids and plasmid incompatibility function(s) appear normal. The presence of covalently closed circular deoxyribonucleic acid in E. coli strains harboring FlacS indicates that it is an autonomously replicating plasmid. Based on beta-galactosidase levels and the percentage of covalently closed circular deoxyribonucleic acid, it appears that the stability of the FlacS is not the result of multiple copies of this plasmid. FlacS appears larger than its precursor, F(ts114)lac, in sedimentation through alkaline sucrose gradients.


Alpha-mannosidase and microcyst differentiation in the cellular slime mold Polysphondylium pallidum. The intracellular and extracellular pattern of alpha-mannosidase (EC 3.2.1.24) activity was studied during microcyst differentiation in the cellular slime mold, Polysphondylium pallidum. The evidence suggests that microcyst differentiation requires continuous protein synthesis. alpha-Mannosidase activity is present in amoebae and increases with differentiation, and the data indicate that this increase in activity requires concurrent protein synthesis. The enzyme is excreted during the differentiation process, and the release of the enzyme is not stopped by cycloheximide. A cystless mutant does not show the normal intracellular pattern of alpha-mannosidase but does excrete the enzyme. Microcyst differentiation is proposed as an alternative system to multicellular slime mold development for the biochemical analysis of certain aspects of cellular differentiation.


Effect of lysozyme on enterococcal viability in low ionic environments. The action of lysozyme on the enterococcal cell differed markedly as a function of the ionic strength of the environment. In high ionic environments (I approximately 0.3), the traditionally slow lytic response and decrease in viability were noted. In a low ionic environment the majority of the cell wall was hydrolyzed, but cellular integrity was preserved and almost all cellular protein, deoxyribonucleic acid and ribonucleic acid remained with the lysozyme-cell complex. However, under these conditions, lysozyme inactivated energy-yielding metabolism, and a rapid extensive loss of viability was observed. Some other basic compounds without lytic activity on the cell wall also effected a substantial reduction in viability. The data suggest that lysozyme acts on the cell membrane to effect disruption of cellular metabolism.


Regulation of flagellar morphogenesis by temperature: involvement of the bacterial cell surface in the synthesis of flagellin and the flagellum. When cells of Proteus vulgaris were transferred from 37 to 42 C, a temperature at which they continue to grow almost optimally, they ceased to form flagella after approximately one generation time. This failure was due to a lesion in the flagellin-synthesizing process rather than the inability of these cells to assemble the organelle from constituents once formed. After transfer back to 37 C, these cells regained their ability to synthesize flagellin and form flagella, after one generation. When added during this period, chloramphenicol, rifampin, or penicillin prevented the synthesis of flagellin. The regeneration of the organelle at 37 C, then, requires growth for one generation, a period during which not only ribonucleic acid and protein synthesis, but also the presence of an intact cell envelope or concurrent synthesis of cell wall, are required.


Growth and macromolecular composition of a mutant of Escherichia coli during polyamine limitation. A mutant of Escherichia coli with reduced levels of biosynthetic arginine decarboxylase was isolated which required putrescine or spermidine for optimal growth. The stimulation of growth by putrescine was 1.5- to 3-fold depending upon the culture medium. Specificity studies supported the concept that the requirement was for spermidine or closely related polyamines, or for diamines which could be converted enzymatically to these compounds. The behavior of the macromolecular composition of the polyamine-starved cells appeared abnormal. The ribonucleic acid to protein and deoxyribonucleic acid to cell ratios in the starved cells were both higher than expected on the basis of their growth rate. The stable ribonucleic acid in the polyamine-limited cells appeared to be normal as judged from size distribution and degree of methylation. The relationship of these results to mechanisms for regulation of nucleic acid and protein synthesis in E. coli is discussed.


Homogeneity of envelope proteins of Escherichia coli separated by gel electrophoresis in sodium dodecyl sulfate. Some envelope proteins of Escherichia coli show variable behavior in acrylamide gel electrophoresis in 1% sodium dodecyl sulfate, depending upon the conditions of the solubilization. When solubilized in 1% sodium dodecyl sulfate at 70 C for 20 min, three distinct peaks (peaks 4, 6, and 7) are seen at molecular weights of 57,800, 44,300, and 38,400, respectively. However, when the envelope fractions are solubilized in 1% sodium dodecyl sulfate at 100 C for 5 min, or when they are treated with N, N-dimethylformamide at acidic pH before solubilization by our method, only a single peak at 48,000 molecular weight is observed in the molecular weight range mentioned above. That is, peaks 4 and 7 disappear and a new peak appears at the position overlapping with peak 6. Proteins isolated from peaks 4 and 7 show the similar molecular weight shifts to the new peak by the treatment at 100 C. No other peaks show any change by the heat treatment. The increase at the new peak is completely accounted for by the decrease at peaks 4 and 7, indicating that the new peak is composed of proteins from peaks 4, 6, and 7. However, it is concluded that these three peaks consist of distinctly different proteins for the following reasons: (i) they have different amino acid compositions, (ii) they show different solubilities in the nonionic detergent, Nonidet P-40, and as shown previously, (iii) peak 6 (protein Y) is related to deoxyribonucleic acid synthesis, and (iv) proteins in peaks 4, 6, and 7 have different resistance to proteolytic enzymes. Although the reasons for the anomalous molecular weight shifts of these peaks are not well understood at present, it is important to solubilize the E. coli envelope proteins by the standard method in order to investigate their properties and functions of the envelope proteins.


Cillary motion in Paramecium. A scanning electron microscope study.


The distribution of synapses on a physiologically identified motor neuron in the central nervous system of the leech. An electron microscope study after the injection of the fluorescent dye procion yellow. The fine structure of a physiologically identified motor neuron in the segmental ganglion of the leech central nervous system and the morphology of synapses on it were studied after injection of the fluorescent dye Procion yellow as a marker. The injected cell and its processes within the neuropil were located in thick or thin sections with fluorescence optics after initial fixation with glutaraldehyde and brief treatment with osmium tetroxide. The same or adjacent thin sections could then be examined in the electron microscope. Comparison with uninjected cells showed that the general features of the injected cell are retained although some organelles are distorted. The main features of the geometry of this neuron are the same from animal to animal: a single large process runs from the soma through the neuropil to bifurcate and enter the contralateral roots. Within the neuropil the main process gives off long branches (up to 150 micro), but these are greatly outnumbered by short branches and spines, one or a few microns in length, which were not appreciated in previous light microscope studies after injection of Procion yellow. Serial thin sections of selected areas along the main process within the neuropil showed that there are synapses on most of the shorter branches and spines; occasional synaptic contacts were also made on the main process itself and on longer branches. At least two morphologically distinct types of synapse could be recognized. A minimum estimate of the total number of synapses on the motor cell is 300, based on their occurrence in reconstructed segments.


Changes in the lipid composition and fine structure of Saccharomyces cerevisiae during ascus formation. Eighty to ninety percent of vegetative cells of Saccharomyces cerevisiae DCL 740 incubated in KCl-acetate medium form asci, the majority of which are four-spored. Ascospores are visible in asci after about 24 hr, and spore formation is complete after about 48 hr. The dry weight of the cells increases by about 75% during 48 hr of incubation, while the lipid content of the cells increases by a factor of four. The increase in lipid content is attributed mainly to an increased synthesis of sterol esters and triacylglycerols and to a lesser extent of phospholipids. The phospholipid and sterol compositions do not change appreciably, but there is a marked increase in the proportion of unsaturated fatty acid residues in ascan lipids. Uniformly labeled (14)C-acetate is incorporated mainly into sterol esters and triacylglycerols and phospholipids. Pulse-labeling by adding acetate-U-(14)C to sporulating cultures and harvesting after a further 6 hr of incubation reveal two main periods of acetate incorporation, namely between 0 and 18 hr, and between 24 and 30 hr. Electron micrographs of thin sections through developing asci show that the principal changes in fine structure occur between 18 and 24 hr and include the appearance of numerous electron-transparent vesicles which become aligned around the meiotic nucleus, and the laying down of extensive endoplasmic reticulum membranes. Changes in fine structure are discussed in relation to the alterations in lipid content and composition of asci.


Calcium binding to intestinal membranes. Flame photometry reveals that glutaraldehyde and buffer solutions in routine use for electron microscopy contain varying amounts of calcium. The presence of electron-opaque deposits adjacent to membranes in a variety of tissues can be correlated with the presence of calcium in the fixative. In insect intestine (midgut), deposits occur adjacent to apical and lateral plasma membranes. The deposits are particularly evident in tissues fixed in glutaraldehyde without postosmication. They are also observed in osmicated tissue if calcium is added to wash and osmium solutions. Deposits are absent when calcium-free fixatives are used, but are present when traces of CaCl(2) (as low as 5 x 10(-5)M) are added. The deposits occur at regular intervals along junctional membranes, providing images strikingly similar to those obtained by other workers who have used pyroantimonate in an effort to localize sodium. Other divalent cations (Mg(++), Sr(++), Ba(++), Mn(++), Fe(++)) appear to substitute for calcium, while sodium, potassium, lanthanum, and mercury do not. After postfixing with osmium with calcium added, the deposits can be resolved as patches along the inner leaflet of apical and lateral plasma membranes. The dense regions may thus localize membrane constituents that bind calcium. The results are discussed in relation to the role of calcium in control of cell-to-cell communication, intestinal calcium uptake, and the pyroantimonate technique for ion localization.


Salmonella typhimurium gastroenteritis in children. A clinical study.


Development of cardiac surgery in Japan.


Amino-acid production by the fermentation process.


Activities of the National Institute of Genetics.


On the supposed "Kenyapithecus africanus" mandible.


Genes coding for polysomal 9S RNA of sea urchins: conservation and divergence.


The bearing of East Rudolf fossils on early hominid systematics.


Risks and benefits of the use of hormonal pregnancy test tablets.


Transperitoneal vesical bivalve in repair of recurrent vesicovaginal fistula.


Cardiac catherization. Author presents a short history of its development as a major diagnostic procedure.


The effect of drugs on immunological renal disease.


Cryopreserved tissue in corneal transplantation.


Skeletal scintigraphy in children.

Skeletal scintigraphy in children.


A brief review. Poststreptococcal glomerulonephritis.


Developments in transient hemorrhagic disorders.


A comparative double-blind study of two antidiarrhoeals, difenoxine and loperamide.


Some ideas and prospects in biomathematics.


Present and future of blood-gas analysis.