Effect of cis-platinous diamminodichloride on graft rejection: prolonged survival of skin grafts against H 2  histocompatibility.


Circulatory factors in energy metabolism of experimental tumors.


Classification of brain tumors and experimental models.


Idiopathic histiocytosis: a discussion of eosinophilic granuloma, the Hand-Schüller-Christian syndrome, and the Letterer-Siwe syndrome.


Pulmonary edema as a complication of increased intracranial pressure.


Advisability of local flaps in facial repair.


Rehabilitation in a nursing home setting.


Newer concepts of reconstructive surgery in patients with cancer of the head and neck.


The location of silver in frog epidermis after treatment by Ranvier's method, and possible implication of the flask cells in transport.


Immunocytological studies on an estradiol sensitive antigen in the cervicovaginal epithelium of neonatal mice.


Chaucer and Doctor John of Gaddesden.

Chaucer and Doctor John of Gaddesden.


A review of cysticercoses of sheep and cattle in Australia.


Arboviruses of Australia.

Arboviruses of Australia.


Selection of beef cattle for tropical Australia.


Arbovirus infections of domestic animals in Australia.


Kenneth Penn Leslie MacGregor.

Kenneth Penn Leslie MacGregor.


Compartmentation in histidine biosynthesis.


Quantitative determination of thiamine and its phosphate esters by Sephadex gel filtration.


Variation of visual acuity in the central region of the retina.


A review of visual screening of schoolchildren.


Receptive field properties of single cells in the dorsal lateral geniculate nucleus of the guinea pig.


Scrotal cancer continues.

Scrotal cancer continues.


Immunosuppression and malignancy.

Immunosuppression and malignancy.


Bright's disease today: the pathogenesis and treatment of glomerulonephritis. 3.


Malaria in the U.K.

Malaria in the U.K.


Detection of endotoxin in commercial L-asparaginase preparations by complement fixation and separation by chromatography.


Ultrastructural changes in rat livers induced by repeated injections of trypan blue.


Alkaline phosphatase activity in spontaneous and induced leukemias in SJL-J mice.


Effectiveness and appearance of resistant faecal E. coli flora during preventive treatment in winter with azidocillin and ampicillin.


Miconazole, a broad-spectrum antimycotic agent with antibacterial activity.


Direct stimulation of lymphoid tissue of the chicken. I. Antibody-producing, antibody-transferring and plaque forming capacity of the thymus, spleen and bursa of Fabricius following intrathymic and intravenous injection of antigen.


Tetanus: a team disease.

Tetanus: a team disease.


The role of T cells and adjuvant in the immune response of mice to foreign erythrocytes.


Georg von Békésy, 1899-1972.

Georg von Békésy, 1899-1972.


Luteinization of rabbit preovulatory granulosa cells cultured in vitro in presence of follicular oocytes. I. Growth characteristics and progestin biosynthesis.


Mouse thymus-independent and thymus-derived lymphoid cells. II. Ultrastructural studies. The ultrastructural features of B-, T-, and surface Ig(sIg)-bearing cells have been studied on cell suspensions from lymphoid organs of mice at different stages of immunization. The cells were identified by exposure to rabbit antibodies against mouse-specific lymphocyte antigens (MSLA) or brain-associated theta antigen (BAtheta) for T cells, mouse-specific bone marrow-derived lymphocyte antigens (MBLA) for B cells, and mouse Ig for sIg-bearing cells. The rabbit antibodies fixed on the cell surfaces were detected by peroxidase-labeled sheep anti-rabbit Ig antibodies or by a "bridge" technique using southern bean mosaic virus or bacteriophage T4 as the final markers. In some experiments, short-lived lymphoid cells were labeled in vivo with repeated tritiated thymidine and the ultrastructural detection of their surface antigens was combined with radioautography. MBLA+ lymphoid cells showed a whole range of ultrastructural patterns. Most were small and medium-sized lymphocytes with a clear cytoplasm containing mono- and polyribosomes, but they comprised also blasts and large cells with various amounts of endoplasmic reticulum, as well as plasma cells at different stages of maturation. sIg-bearing cells appeared to be identical with MBLA+ cells, except that sIg was less easily detectable on large blasts, and only very rarely observed on plasma cells. MSLA+ and BAtheta+ cells fell into three categories. One of them (T(1) cells) consisted of small to medium-sized lymphocytes with a clear cytoplasm and few organelles, mostly monoribosomes. A second consisted of large cells (T(2) cells) characterized by numerous polyribosomes often in a "rosette"-like pattern, occasional dark, membrane-bound granules, and a developing "filamentous network." The third, very characteristic type, (T(3) cells) was represented by dark small to medium-sized lymphocytes, usually containing large amounts of closely packed ribosomes and showing a striking accumulation of filamentous network, often condensed in large areas devoid of cell organelles. This filamentous network appeared to correspond to the cytochalasin B-sensitive system of microfilaments found in other cells and considered to be one of the contractile elements of the cell. The T(3) lymphocytes showed frequently vesicles suggestive of a strong pinocytic activity, and assumed a variety of shapes, including uropods. Evidence is presented that T(1) lymphocytes represent "virgin" T cells, T(2) "activated," and T(3) "differentiated" lymphocytes.


Heparin treatment of live Escherichia coli bacteremia in rats.


Regulation of antibody response by cells expressing histamine receptors. Spleen cells from immunized and unimmunized mice were either passed over histamine-rabbit serum albumin-Sepharose columns or rabbit serum albumin-Sepharose control columns. The immune response potential of 5 x 10(6) cells excluded from the two columns were compared with each other, and with an equal number of unfiltered cells by injection of the cell suspensions mixed with sheep erythrocytes into irradiated, syngeneic recipients. The direct and indirect anti-sheep erythrocyte plaque-forming cell responses generated by the cells passed over the histamine-bead column were significantly greater than the responses resulting from the inocula of unfiltered cells or cells passed over control columns. These results indicate the existence of a cell population expressing surface receptors for histamine, which functions to regulate antibody responses.


Immune responses in vitro. VI. Genetic control of the in vivo-in vitro discrepancies in 19S antibody synthesis. The finding that the relationship of the in vitro and in vivo responses of different strains of mice is under genetic control indicates that at least two mechanisms must operate under in vivo conditions to control 19S antibody synthesis. One is involved in the termination of 19S antibody synthesis; the other has a regulatory role on the magnitude of the response. In light of these findings, various concepts based on other genetically controlled immune responses and on the limiting dilution technique should be reassessed. Furthermore, the suppressive in vivo mechanism may be an important type of control in the resistance or susceptibility to the establishment or maintainance of neoplasms.


Serum enzymes in chronic hemodialyzed patients.


Urinary extravasation following renal transplantation: a case report and review of the literature.


Functional ureteral obstruction and massive diuresis following renal homotransplantation.


A controlled study of prindolol (Visken) in hypertension.


Frederic Wood Jones.

Frederic Wood Jones.


The role of transferrin in iron metabolism.


Keith Lindsay Hugh Kirkland.

Keith Lindsay Hugh Kirkland.


Burns contractures in the child.

Burns contractures in the child.


Drowning and the diving reflex in man.

Drowning and the diving reflex in man.


Treatment of apparently early breast cancer. 2. Where we are going.


Physical activity and cardiovascular health. I. Potential for prevention of coronary heart disease and possible mechanisms.


Specificity of sigma-dependent binding of RNA polymerase to DNA.


Genetic control of the arginine pathways in Aspergillus nidulans. Common regulation of anabolism and catabolism.


Studies on lambda virulent mutants. I. Isolation and characterization of revertants from lambdavirC mutant.


Serum creatine kinase in infantile malnutrition.


Pitfalls in calculating the vitamin E content of diets.


Alcoholism and folic acid.

Alcoholism and folic acid.


Adipose cell size and number in experimental human obesity.


Bioflavonoids as a growth factor for the cricket.


An introduction to homograft rejection and its control by irradiation.


Erythrocyte composition in Plasmodium falciparum infection in man.


Studies on fibrinolytic activity in patients with Plasmodium falcipalum malaria.


Tolnaftate in the treatment of superficial mycotic infections.


Cross species immunosuppressive effects of antilymphocyte serum in mice and rats.


The SL-A histocompatibility system in the Sus scrofa species.


Prolonged survival of heart allografts transplanted in rats and rabbits following pregnancy.


Accelerated rejection of skin homografts by vitamin A acid.


Hyperacute renal allograft rejection in the rabbit. Passive transfer, specificity, and prevention.


The influence of DL-A histocompatibility on the function and pathohistological changes in unmodified canine renal allografts.


Comparison of skin allograft rejection and cytotoxic antibody production in two lines of mice genetically selected for "high" and "low" antibody synthesis.


The effect of donor strain blood pretreatment on renal allograft rejection in rats.


Transplantation reactions of tropical Australian echinoderms.


Secretory function and relative resistance of the gastric mucosa of the rat to allogeneic rejection.


Immunosuppressive effect of rifampicin.

Immunosuppressive effect of rifampicin.


Evidence for adaptation of skin grafts in enhanced irradiated mice.


Paul Hamilton Wood.

Paul Hamilton Wood.


Observations on the behavior of recipient atria after cardiac transplantation in man.


Arteriotomy in children during cardiac catheterization.


Adolf Fick--forgotten genius of cardiology.


A comparison of multiple-test systems for the presumptive identification of enterobacteriaceae.


The futility of thioglycollate inoculation to recover anaerobes from urine specimens.


Rapid differential testing of the enterobacteriaceae using modified standard media.


Anaerobic bacteriology in the clinical laboratory.


Timely topics in general microbiology, 1968-1970: infections.


Procedure for determining heptose and hexose in lipopolysaccharides. Modification of the cysteine-sulfuric acid method.


An automated system for cell cycle analysis.


Comparison of different techniques for isoelectric focusing on polyacrylamide gel slabs using bacterial asparaginases.


The annulate lamellae of chick embryo liver cells in organ culture.


Ultrastructure of spermine-treated Escherichia coli, including a polar organelle concerned with envelope synthesis.


Lack of virus-induced cell surface antigen in metaphase-arrest cells infected with poxvirus.


Transport of  -aminoisobutyric acid in Saccharomyces cerevisiae.


A showdomycin-resistant mutant of Escherichia coli K-12 with altered nucleoside transport character.


Extraction of lipids from serum and measurement of total serum lipids.


Humidification in anaesthesia. A review of the present situation.


Comparison of the sedative effects of diazepam given by the oral and intramuscular routes.


Quantitative evaluation of the surfactant system of the lung.


An evaluation of metoclopramide (Maxolon) as an anti-emetic in minor gynaecological surgery.


Twenty-five years of proficiency testing for clinical laboratories.


The measurement of total fatty acid in serum.


Serum cholesterol in health and disease.


Purification and properties of DNA-dependent RNA polymerases from yeast.


Utilization of methionine-accepting tRNA species form Escherichia coli, ascites-tumor cells, and yeast in homologous and heterologous cell-free systems.


Aflatoxin residues in food of animal origin.


Long-term effects of plastics.

Long-term effects of plastics.


No smoke without benzopyrene.

No smoke without benzopyrene.


Mainly good news about asbestos.

Mainly good news about asbestos.


The blessings of zinc.

The blessings of zinc.


Primary care in the industrial areas of Britain. Evolution and current problems.


Physiologic and histologic response to bone cutting with rotary instruments. A review of the literature.


Yersinia enterocolitica infection: report of two cases.


Automated fluorescent treponemal antibody (AFTA) test for cerebrospinal fluids in the diagnosis of neurosyphilis.


Physiologic and metabolic factors in the pathogenesis of neonatal enteric infections in calves.


Study of the maturation of 5 s RNA precursors in Escherichia coli.


Synthesis and decay of messenger ribonucleic acid from the lactose operon of Escherichia coli during amino-acid starvation.


Biosynthesis of Escherichia coli aspartate transcarbamylase. I. Parameters of gene expression and sequential biosynthesis of the subunits.


Interaction of the recombination pathways of bacteriophage lambda and host Escherichia coli: effects on lambda recombination.


Interaction of the recombination pathways of bacteriophage lambda and its host Escherichia coli K12: effects on exonuclease V activity.


Origin and sequence of chromosome replication in Escherichia coli.


Identification of the T4rIIB gene product as a membrane protein.


Role of genes 46 and 47 in bacteriophage T4 reproduction. II. Formation of gaps on parental DNA of polynucleotide ligase defective mutants.


Mirth control for the medical computer.

Mirth control for the medical computer.


The agony and the ecstasy of the nephrologist.


Treatment of apparently early breast cancer. I. The dilemma.


Aubrey Arthur King.

Aubrey Arthur King.


A modification of the oxidation-fermentation test for the classification of Micrococcaceae.


The localization of infrequent tissue structures for electron microscopy: a technique providing thick sections of plastic-embedded tissue.


Transport of urine for bacteriological examination.


A safety container for use when freeze-drying infected materials.


Physical activity and cardiovascular health. 3. The exercise prescription: frequency and type of activity.


Auxiliary liver transplantation in acute liver failure.


Coronary bypass operations for ischemic heart disease.


An analysis of pediatric surgery in North America and Northwestern Europe.


The bilateral Martius pedicle graft. Surgical adjunct in secondary procedures for stress incontinence.


The pig in pediatric surgical research.

The pig in pediatric surgical research.


Holoacardius.

Holoacardius.


Actin detected in mouse neuroblastoma cells by binding of heavy meromyosin.


Some phono-stylistic featues of Black English.


Marital pathology. A review.

Marital pathology. A review.


Physical form of the diet in relation to rumen fermentation.


Fate of metabolic hydrogen in the rumen.


The control of fermentation of carbohydrate.


Control of nitrogen metabolism in the ruminant.


The protection of dietary components from rumen fermentations.


Significance to the host of changes in fermentation activity.


Intestinal absorption of amino acids and peptides.


Control by insulin of protein synthesis in muscle.


Secretion of human growth hormone.

Secretion of human growth hormone.


Insulin and muscle amino acid balance.

Insulin and muscle amino acid balance.


Metabolism of alcohol (ethanol) in man.

Metabolism of alcohol (ethanol) in man.


Malignancy and immunosuppression.

Malignancy and immunosuppression.


The pharmacological actions of alcohol in relation to nutrition.


Myeloma and leukaemia trials.

Myeloma and leukaemia trials.


The complementary roles of ENT and plastic surgery in major malignancies of the middle ear.


Effect of ICRF 159 on immune responses.

Effect of ICRF 159 on immune responses.


Serum immunosuppressive activity of azathioprine in normal subjects and patients with liver diseases.


Renal preservation.

Renal preservation.


Tissue typing for cadaveric transplantation.


Cellular basis of antibody production.

Cellular basis of antibody production.


Lysosomes and mental retardation.

Lysosomes and mental retardation.


Comparison between alprenolol and propranolol as antihypertensive agents. A double-blind cross-over study.


New aspects of the coronary circulation.


Articular cartilage plasty.

Articular cartilage plasty.


A clinical trial of inhaled inactivated influenza vaccine.


Double-blind trial with increasing doses of salbutamol and terbutaline aerosols in patients with reversible airways obstruction.


A double-blind comparison of slow-release tetracycline and tetracycline hydrochloride in purulent exacerbations of chronic bronchitis.


Sulfametopyrazine in the prophylaxis of exacerbations of chronic bronchitis.


But for Tom Dooley.

But for Tom Dooley.


Trial of a method of self-screening for gonorrhoea suitable for apprehensive girls.


CNS--an on-line program for controlling stereotaxic stimulation within the central nervous system, and for recording and analysis of responses in a neurophysiological experiment.


Influence of cutaneous sensitization with oxazolone on recruitment of myelogenous stem cells in the thymus.


Immunglobulin classes, subclasses and complexes of IgG rheumatoid factor in rheumatoid plasma cells.


Autoimmunity in a rural community.

Autoimmunity in a rural community.


AM-12, a program for simplified discriminant analysis on a small computer.


Nucleotide sequences of fragments of R17 bacteriophage RNA from the region immediately preceding the coat-protein cistron.


The isolation and characterization of nerve-growth factor from the venom of Vipera russelli.


Biological indicators for steam sterilization--a U.S.P. collaborative study.


A series of specific ribonucleoprotein fragments from the 30-S subparticle of Escherichia coli ribosomes.


ato Operon: a highly inducible system for acetoacetate and butyrate degradation in Escherichia coli.


Transcription in vitro of T3 DNA by Escherichia coli and T3 RNA polymerases. Analysis of the products in cell-free protein-synthesizing system.


The separation of sub-populations of T and B lymphocytes.


Lipopolysaccharide can substitute for helper cells in the antibody response in vitro.


Induction of immunoglobulin and antibody synthesis in vitro by lipopolysaccharides.


Recall of antibody synthesis to the primary antigen following successive immunization with heterologous albumins. A two-cell theory of the original antigenic sin.


Induction of antibody-forming cell clones in microcultures.


Cholera therapy in the nineteenth century.


Joseph Black matriculates: medicine and magnesia alba.


Inoculation: pro and con.

Inoculation: pro and con.


Cardiopulmonary resuscitation in the eighteenth century. A historical perspective on present practice.


Danilo Samoilowitz: an eighteenth-century Ukrainian epidemiologist and his role in the Moscow plaque (1770-72).


Hephaestus and clubfoot.

Hephaestus and clubfoot.


Percussion and nostalgia.

Percussion and nostalgia.


A technique of aortic valvuloplasty for aortic insufficiency associated with ventricular septal defect.


Assessment of indicator-dilution method of measuring valvular insufficiency.


The management of interstitial pulmonary edema. Significance of hypoproteinemia.


Computers, professional responsibility and the privacy of the individual.


Electrographic studies of a new volatile anesthetic agent: enflurare (ethrane).


The use of a new vasoconstrictor in the management of burns.


A history of general practice in Australia.


Biological synergism between the LDH-virus and Eperythrozoon coccoides: studies on the mechanism.


American Physiological Society membership.


Reaction of N-acetylimidazole with L-asparaginase.


The surgery explosion: from syme to eternity.


Past-president's address. A reconsideration of the "Biological Clock in the Unicorn".


Allan Hemingway 1902-1972.

Allan Hemingway 1902-1972.


F.J.W. Roughton, F.R.S. 1899-1972.

F.J.W. Roughton, F.R.S. 1899-1972.


Rapid transfer of thoracoacromial flaps to the face and neck.


The surgical treatment of bedsores in paraplegics.


Growth of skin grafts, flaps, and scars in young minipigs.


Honorary award of the American Association of plastic Surgeons to Dr. Jerome P. Webster. Presentation speech.


Iliac bone graft to bridge a mandibular defect. Forty-nine-year clinical and radiological follow-up.


Reconstruction of the nasolabial fold.

Reconstruction of the nasolabial fold.


The classic reprint. Experimental study of fractures of the upper jaw. I and II. René Le Fort, M.D.


Michel skin clips for rapid fixation of split-skin grafts to sharp edges of deep defects.


Reconstructive rhinoplasty with auricular composite graft and forehead flap with periosteum. Case report.


Repir of coup de sabre, a linear form of scleroderma. Case report.


Studies on candida.

Studies on candida.


The lying-in hospital 1747.

The lying-in hospital 1747.


Infection after cadaveric renal transplantation.


Vitamin A and protein metabolism.

Vitamin A and protein metabolism.


Urinary fistulae following renal transplantation.


Renal transplant rejection detected with  125 I-fibrinogen.


Smooth-muscle autoantibodies, viral infections and malignant disease.


Polymyalgia rheumatica.

Polymyalgia rheumatica.


Incidence of emphysema at necropsy as assessed by point-counting.


William Goodell, M.D., and the Preston Retreat.


Haemodynamic effects of glucagon after mitral valve replacement.


J. Edwards Lee, M.D., and the founding of the Wisconsin State Hospital.


Dr. Peter Pineo (1825-91): Cape Cod surgeon.


Memoir of Samuel Bellet, 1902-71.

Memoir of Samuel Bellet, 1902-71.


Memoir of Richard Joseph Campion, 1894-1971.


Memoir of Leon H. Collins, Jr., 1899-1972.


Memoir of William Lawrence Estes, Jr., 1885-1971.


Memoir of E. Harold Hinman, 1904-71.

Memoir of E. Harold Hinman, 1904-71.


Memoir of S. Leon Israel, 1906-71.

Memoir of S. Leon Israel, 1906-71.


Memoir of Joseph W. Spelman, 1918-71.

Memoir of Joseph W. Spelman, 1918-71.


Memoir of Joseph Stokes, Jr., 1896-1972.


The Adam Politzer and Joseph Hyrtl otologic collections at the Mütter Museum.


Memoir of Jacob Earl Thomas, 1891-1972.

Memoir of Jacob Earl Thomas, 1891-1972.


Women in nineteenth century Wisconsin medicine.


The use of the Gerber attachment.

The use of the Gerber attachment.


The tRNA methyltransferases.

The tRNA methyltransferases.


Removable periodontal prosthesis: the crown and sleeve-coping retainer.


A review of some common sources of error in the preparation of agar media.


A suggested method for evaluating the effective application of antiseptics in laundered fabrics.


The inhibition of glycosidases by aldonolactones.


Adhesives in dentistry--the state of the art.


Choledochometry and trans-duodenal sphincterotomy.


Metabolic roles of fat-soluble vitamins D, E, and K.


Memoir of Edward Steinfield, 1890-1972.

Memoir of Edward Steinfield, 1890-1972.


Biochemistry as part of my life.

Biochemistry as part of my life.


Mechanism of action of the female sex hormones.


Mitochondrial nucleic acids.

Mitochondrial nucleic acids.


Corrinoid-dependent enzymic reactions.

Corrinoid-dependent enzymic reactions.


Restriction and modification of DNA.

Restriction and modification of DNA.


Glycoproteins.

Glycoproteins.


Lipid bilayers and biomembranes.

Lipid bilayers and biomembranes.


Fluorescence probes for structure.

Fluorescence probes for structure.


Conformation and conformational transitions of poly- -amino acids in solution.


Hydrogen exchange.

Hydrogen exchange.


Configurational and conformational aspects of carbohydrate biochemistry.


Functionally generated paths in occlusal surface design.


Injected apomorphine and orally administered levodopa in Parkinsonism.


Sympathetic neural influence on norepinephrine vasoconstriction in brain vessels.


Double-blind clinical trial with rifampicin versus ampicillin in the control of chronic bacteriuria.


Subacute sclerosing panencephalitis. Transmission of the virus to calves and lambs.


Marrow transplantation and iron therapy in mouse hereditary microcytic anemia.


The effect of osmolality on the response of Escherichia coli and Proteus mirabilis to penicillins.


The fate of cutaneously and subcutaneously implanted trypsin purified dermal collagen in the pig.


Tumour growth and non-specific immunity in rats: the mechanisms involved in inhibition of tumour growth.


The fibromatoses: a clinicopathological concept.


Paget's disease in a 5-year-old: acute response to human calcitonin. A five-year-old boy presented with a three-and-a-half-year history of repeated bone fractures and progressive bone deformity. The excretion of hydroxyproline in the urine was greatly increased, and serum alkaline phosphatase and acid phosphatase levels were very high. These abnormalities together with the findings on bone histology and radiology suggested a diagnosis of juvenile Paget's disease. Human calcitonin reduced the bone turnover as evidenced by an immediate and sustained fall in urine hydroxyproline excretion, while calcium and phosphate balance became more positive. This treatment is therefore being continued on an outpatient basis.


Hereditary nephritis.

Hereditary nephritis.


Cincinnati Dental Society 1972 directory.


Positive end-expiratory pressure (PEEP); indications and physiologic considerations.


The influence of intrathoracic pressure on fluid and electrolyte balance.


Role of bronchopulmonary lavage in the treatment of respiratory failure: a review.


Spasmodic croup in children. Personal experiences with intermittent positive pressure breathing in therapy.


Which pediatrichians take throat cultures? What is done with the information? A survey of private practitioners in New Jersey.


Some important abuses practiced upon the valerian. An account of drug adulteration in the 18th century.


Isolation and characterization of a serum antibactericidal factor.


The management of deformities following electric fire burns.


Thumb web contracture.

Thumb web contracture.


Decrease of glycogen and trehalose in yeast during starvation and during ethanol formation under the influence of propanol or ethanol.


Difference in the target cells for tolerance induction in relation to the dose of tolerogen.


Lymphoid tissue architecture. Experimental analysis of the origin and distribution of T-cells and B-cells.


Reversible loss of surface receptors on lymphocytes.


The ability of thymus and bone marrow cells from immunized donors to restore antibody formation to BSA in irradiated mice.


Role of macrophages in tumour immunity. II. Involvement of a macrophage cytophilic factor during syngeneic tumour growth inhibition.


Immunologic modification of runt disease in rats.


Cultivation of rat bone marrow. II. Long-term erythrocyte production in vitro.


Assessment of cyclobenzaprine in the treatment of spasticity. The efficacy of cyclobenzaprine 60 mg/day in the treatment of spasticity was assessed in a double-blind crossover trial of two weeks' duration in 15 patients suffering from cerebral or spinal spasticity. Independent clinical and electromyographic methods were used. The effects of cyclobenzaprine did not differ significantly from those of placebo. The administration of a higher dosage, 150 mg/day, to one patient revealed a dose-related response, but the degree of improvement was clinically small. Apart from a skin rash there were no significant untoward effects of therapy.


The use of electrically conductive glue in mounting sppecimens for the scanning electron microscope.


Physical activity and cardiovascular health. II. The exercise prescription: intensity and duration.


Methods of designing, elevating, and suturing the intraoral flap.


Manual on cardiac catheterization in adults with acquired and congenital heart disease.


Precursors and prevention of coronary artery disease. An overview.


Parkinson's disease. Modification by 5-hydroxytryptophan.


Fables for the instruction of youth published in Boston in 1818. Excerpt V. Wolves and the sheep.


Milled porcelain veneer precision attachments in mandibular restoration.


Dr. and Mrs. Alfred Washburn.

Dr. and Mrs. Alfred Washburn.


Difficulties of subgingival impression taking with elastomeric materials. (I).


Microspectrophotometry of visual pigments.


Heroin intoxication in adolescents.

Heroin intoxication in adolescents.


Pathophysiology of gastritis: recent concepts and clinical significance.


Human liver transplantation without azathioprine.


Role of pulmonary microembolism in the hemodynamics of endotoxin shock.


Portal inflow and auxillary hepatic allograft function.


Immediate mucocutaneous suture for loop colostomy.


Ureteral replacements.

Ureteral replacements.


A review of some effects on health and performance of variation in the physical form of the diet of the growing pig. 3. Heat treatments and cubing.


A review of tranquillisation and sedation in large animals.


Tanderil in surgery of rheumatic diseases. A double blind trial at dorsal synovectomy of the wrist joint.


Programmed learning in the treatment of severely disturbed children: the role of motor patterning.


Double Y chromosomes in a boy from a maladjusted family. A case report.


Is there a common psychopathology of XYY boys? A clinical report on three cases of XYY and one of XY-XYY.


Automated, high-resolution analyses for the clinical laboratory by liquid column chromatography.


Acid phosphatase.

Acid phosphatase.


Mechanisms in protein synthesis. 8. Competitive inhibition of polyphenylalanine synthesis by non-poly (U)-coded aminoacyl-tRNAs.


Demonstration of two tyrosyl-tRNA synthetases of pea roots.


Polynucleotides,. XIV. Synthesis and properties of polynucleotides containing 2,6-bis(methylthio)purine ribonucleotides.


Vernamycin A inhibits the non-enzymatic binding of fMet-tRNA to ribosomes.


A double-blind crossover trial comparing a new antirheumatic agent alclofenac with phenylbutazone in chronic rheumatic disorders.


Enhanced relief of phobias by flooding during waning diazepam effect.


Aspects of clinical psychiatry in Sub-Saharan Africa.


A comparison of oxypertine and diazepam in anxiety neurosis seen in hospital out-patients.


The way of the innovator: notes toward a prehistory of MEDLARS. The concept of MEDLARS as a publication/retrieval system was the product of several forces operating during the fifteen years prior to 1961. These included the overriding imperative of medical index publication, the Welch Medical Indexing Research Project, a drive to master the theory of medical subject headings, the innovative impact of coordinate indexing, and the confidence produced by the success of the Index Mechanization Project. By virtue of this experience, MEDLARS became a prime example of a well-understood enterprise seeking a computer application, a circumstance which augured of its success.


The old library of the Pennsylvania Hospital. The first medical book acquired by the Pennsylvania Hospital was William Lewis's Experimental History of the Materia Medica. Dr. John Fothergill of London donated it in 1762. The next year the Managers resolved to establish a library, and the physicians assigned their fees from hospital students for the purchase of books. Books were regularly ordered from William Strahan in London, and gifts and bequests added still more volumes. From 1790 to 1810 Dr. John Coakley Lettsom of London was the hospital's principal adviser on purchases. As the library grew, it expanded beyond purely medical works to include some in natural history, many purchased in 1817 from the estate of Dr. Benjamin Smith Barton. Catalogues were printed in the years 1790-94, 1806, 1829, and 1857. At midcentury the hospital library numbered about 9,000 volumes. At that time it was the largest medical library in the United States.


Historical chronology and selected bibliography relating to the National Library of Medicine.


Nutrition information sources.

Nutrition information sources. Medical personnel and medical librarians may tend to think of nutrition in medical terms and to forget its interdisciplinary aspects. For this reason, it is desirable for medical librarians to become familiar with a variety of sources of information on the composition of foods, nutrient values, food additives, and food protection. Many of these are government publications from such agencies as the U.S. Department of Agriculture, the National Research Council, and the Food and Agriculture Organization. Less familiar sources include nutrition materials from state agricultural experiment stations and extension services and important data published in a wide range of scientific or agricultural journals, which may be located through Nutrition Abstracts and Reviews, Food Science and Technology Abstracts, and the Bibliography of Agriculture. Sources of current information on nutrition research in progress include the Department of Agriculture's Current Research Information System (CRIS) and selective listings from the Smith-sonian Information Exchange.


The detection of fibrinogen degradation products (FDP) in serum and urine.


Clinical application of a new stereotaxic instrumentation.


Research needs in leptospirosis.

Research needs in leptospirosis. Leptospirosis is a widespread infection of man and animals, and locally it assumes considerable importance as a public health and economic problem. It is an important occupational infection among persons working with animals or in environments that may be contaminated by infected animals. In recent years, considerable attention has been devoted to this infection but efforts to control and eliminate it, especially from natural foci, are hindered by gaps in our knowledge. This memorandum is a review of recent progress and current problems in leptospirosis research, with special reference to taxonomy, epidemiological methods, and control measures. Certain aspects of the basic biology of leptospires that are relevant to these topics are also discussed.


Structural-functional organization of the human brain and the pathophysiology of the Parkinsonian type hyperkineses.


Ten year follow-up of a case of Raynaud's disease treated by pallidotomy.


The effects of stereotaxic subthalamotomy on sympathetic tonus.


Long-term effects on verbally expressed cognition following left and right ventrolateral thalamotomy.


Stereotaxic amygdalotomy in temporal lobe epilepsy.


Anatomical correlation of detailed sensory mapping of the human thalamus.


Sagittal thalamotomy for relief of motor disorders in cases of double athetosis and cerebral palsy.


Somatotopic array of the ventro-oral nucleus of the thalamus based on electrical stimulation during stereotactic procedures.


Histological and clinical correlations in 29 Parkinson patients with stereotaxic surgery.


Electromyographic study of motor responses following electrical stimulation of the corticospinal tract in man during stereotaxy.


Radiosurgical thalamotomy for intractable pain.


Interstriatal relationships. An electrographic study.


Results of stereotaxic thalamotomy for pain.


Stereotaxic lower cervical cordotomy for the treatment of intractable pain.


Postencephalographic psycho-organic syndrome in Parkinsonism.


Dentatotomies or thalamotomies in the treatment of hyperkinesia.


The H-reflex in patients with Parkinson syndrome before and after stereotaxic procedures and drug administration.


Long-term results of stereotactic treatment of spasmodic torticollis.


Hypokinetic syndromes and their therapy.


Stereotaxic treatment of extrapyramidal myoclonus.


Neurophysiological findings during stereotactic operation in thalamus and subthalamus.


Clinical picture of cerebral palsy in neurological understanding.


Correlation between anatomically calculated target point and physiologically determined point in stereotaxic surgery.


Computer calculation of the position of the side-protruding electrode tip during penetration in human brain.


The long-term distribution of antibody-forming cells.


Structure of the lymphocyte membrane. II. Evidence of two classes of Ig on the surface of rabbit lymphocytes during primary response to sheep red blood cells using inhibition of rosette-forming cells and combined ultrastructural autoradiographic and peroxydase methods.


Isoelectric focusing spectra of antibodies which activate mutant  -galactosidases.


The detection of theta antigen on cells by immunofluorescence: failure to detect theta antigen on rosette-forming cells.


Lymphocyte stimulation and interferon induction by 7 S anti-human lymphocyte globulins and their uni- and divalent fragments.


Toxicity studies on a microbial rennet.

Toxicity studies on a microbial rennet.


Touching up the paraquat picture.

Touching up the paraquat picture.


Unravelling analgesic nephropathy.

Unravelling analgesic nephropathy.


Genetic control of metabolism: enzyme studies of the obese and adipose mutants in the mouse.


A genetic map of several mutations affecting the mucopeptide layer of Escherichia coli.


A study of hemolysin produced in two strains of Clostridium tetani.


Evolution life cycle of the human pathogen Treponema pallidum.


A new medium for Bacillus thuringiensis Berliner.


A waterborne actinomycete resembling strains causing mycetoma.


The fluorescent antibody technique for the detection of Salmonella in routine use.


Influence of various factors on the survival of Lactobacillus leichmannii during freezing and thawing.


Malt extract as protective medium for lactic acid bacteria in cryopreservation.


A survey of the occurrence of Vibrio parahaemolyticus and V. alginolyticus on mussels and oysters and in estuarine waters in the Netherlands.


The ecology and classification of strains of Lactobacillus collinoides nov. spec.: a bacterium commonly found in fermenting apple juice.


Localization of the septal rage syndrome in Long-Evans rats.


Genes, enzymes and membrane proteins of the nitrate respiration system of Escherichia coli.


A bacteriophage T4 mutant defective in protection against superinfecting phage.


Primary health care and the poor in the inner cities of New York and Washington, U.S.A.


Sulphadimidine, co-trimoxazole, and a placebo in the management of symptomatic urinary tract infection in general practice.


Isolation and characterization of a lysogenic strain of Nocardia erythropolis. A stable phage-carrying strain of Nocardia erythropolis was isolated from an infection with the nocardiophage phiEC. Growth of the strain in phage-specific antiserum for 48 hr produced cured organisms at a frequency of about 0.5%. Spontaneous curing, determined by serial single-colony isolations, was less than 0.4%. The strain could not be infected by phage phiEC nor by a closely related phage, phiC, although the cells were able to adsorb these phages. In cell populations, a frequency of 2.5 x 10(-4) cells spontaneously induced. The growth rate of the strain was comparable to that of the uninfected wild-type N. erythropolis. Ultraviolet irradiation or treatment with mitomycin C induced the strain to produce larger numbers of phage. It was concluded that the isolated strain was lysogenic.


Isolation and characterization of bacteriophage T4 base plates. A method for isolating bacteriophage T4 base plates from lysates of Escherichia coli B cells infected with the ts mutant in gene 19, ts B31 has been developed. By electrophoresis in polyacrylamide gel with sodium dodecyl sulfate the base plates have been shown to contain five to seven protein components with molecular weights of 36,000, 53,000, 66,000, 81,000, 87,000, and probably about 100,000. Electron microscope studies have demonstrated that base plates may occur in two structural states: in the form of hexagons or stars. Star rays and short fibrils are not radial or elongated and are turned sideways at an angle to the radius. Base plates do not complement in vitro with free tail cores isolated after disintegration of particles of the wild-type bacteriophage.


Behavior of coliphage lambda in Shigella flexneri 2a. The insensitivity of wild-type Shigella flexneri 2a to coliphage lambda is a consequence of its native genetic defect in the malA gene cluster. The "smooth" S. flexneri 2a lipopolysaccharide layer affects the efficient adsorption of lambda. Derivatives, capable of serving as functional hosts for lambda, were obtained by repairing the malA lesion, enabling the expression of the malB-lambdarcp region of S. flexneri. Introduction of a mutation into S. flexneri causing a "rough" lipopolysaccharide character resulted in more efficient adsorption of lambda. Such S. flexneri hosts can be stably lysogenized and upon induction yield gal(+)-transducing lysates. Lambda propagated on a malA(+) rough S. flexneri host was restricted by Escherichia coli K-12 and E. coli B, but not by E. coli C. This S. flexneri host did not restrict lambda grown on these E. coli strains.


Effect of bacteriophage ghost infection on protein synthesis in Escherichia coli. The rate of protein synthesis by Escherichia coli markedly decreased within 1 min after phage T4 infection, whereas a complete cessation of protein synthesis was observed within at least 25 sec after T4 ghost infection. The cellular level of amino acids and aminoacyl-transfer ribonucleic acid (tRNA) did not change drastically upon infection with ghosts, indicating that the inhibition of protein synthesis took place at a step(s) beyond aminoacyl-tRNA formation. The host messenger RNA remained intact and still bound to ribosomes shortly after ghost infection. Kinetic studies of the effect of ghosts on host protein synthesis revealed that nascent peptide chains on ribosomes were not released upon ghost infection.


Role of F pili in the penetration of bacteriophage fl. Early stages of infection of Escherichia coli with the filamentous bacteriophage f1 were examined in the electron microscope. Purified phage-bacteria complexes were prepared at various time intervals after the initiation of synchronous infection. Cells were scored for the total number of F pili, the number of F pili with f1 attached, the number of intact phage particles which occurred at the surface of the cell, and F pilus length. Electron microscope autoradiographs were also prepared at each time interval. The results showed that the average number of F pili with f1 attached decreased with time as phage deoxyribonucleic acid (DNA) entered the cell. Concomitant with this loss, the remaining F pili became shorter. The rate of entry of phage DNA into the cell followed, with a short lag, the rate of loss of F pili with f1 attached. During the lag period, intact phage particles accumulated at the surface of the cell. The results from radioautographs showed that no phage DNA could be located within the F pilus. These results suggest that F pili are resorbed by the cell during infection with the bacteriophage f1. Parallel experiments with noninfected cultures further suggest that pilus resorption may be a normal cellular phenomenon.


Defective concatemer formation in cells infected with deoxyribonucleic acid-delay mutants of bacteriophage T4. Nonpermissive host cells infected with phage T4 mutants in genes 52, 39, and 58 through 61 are shown to form short intracellular single-stranded deoxyribonucleic acid in contrast to wild-type infected cells, which form dimers and trimers of T4 genome size.


Effect of Zn 2+  on the adsorption of male-specific filamentous deoxyribonucleic acid and isometric ribonucleic acid bacteriophages. By means of both electron microscopy and plaque assay techniques, 10(-3)m Zn(2+) has been shown to reduce the adsorption rate of male-specific filamentous deoxyribonucleic acid bacteriophages to the tips of F pili. In contrast, 10(-3)m Zn(2+) did not affect the adsorption of male-specific ribonucleic acid phages to the sides of F pili.


Location of ferritin-labeled concanavalin A binding to influenza virus and tumor cell surfaces. Concanavalin A (Con-A) was linked to ferritin with glutaraldehyde and chromatographed on Sepharose 6B to separate unconjugated Con-A and ferritin from covalently cross-linked molecules. Ehrlich ascites tumor cells were infected with WSA influenza virus, stained at intervals with the ferritin-labeled Con-A and examined by electron microscopy. The surfaces of most mature viruses were specifically stained, providing direct evidence that influenza viruses maturing in this cell type have exposed Con-A receptor sites. The ferritin cores of the staining reagent were found at an average distance of 21.3 nm from the virus membrane and 10.8 nm from the uninfected cell membrane. This finding was interpreted to mean that the population of Con-A receptor sites on influenza virus particles is located at an average distance from the virus membrane twice that of the population of Con-A receptor sites found on uninfected cells. The structural elements of viral membranes can provide a reliable means for evaluating electron microscopy staining reagents, thereby enhancing their usefulness as probes for the study of membrane relationships.


Exclusion of bacteriophages by T2 ghosts. T2 ghosts do not exclude T4, T7, or lambda-induction in Escherichia coli which survive ghost infection. Latent periods are extended, probably by the temporary inhibition of protein synthesis.


Principles of bacterial taxonomy.

Principles of bacterial taxonomy.


Genetical approaches to taxonomy.

Genetical approaches to taxonomy.


An autograph letter of Dr. Renĕ Laennec.


History and the half-dead disease. Campbell oration 1972.


Abortion and contraception in Sweden 1870-1970.


Correlation of computer-quantitated treadmill exercise electrocardiogram with arteriographic location of coronary artery disease.


Samuel D. Robbins' autobiographical reminiscences.


The use of deoxyfluoro-D-glucopyranoses and related compounds in a study of yeast hexokinase specificity. 1. 2-Deoxy-2-fluoro-d-glucose, 2-deoxy-2-fluoro-d-mannose and 2-deoxy-2,2-difluoro-d-arabino-hexose are good substrates for yeast hexokinase. 2. 3-Deoxy-3-fluoro-d-glucose and 4-deoxy-4-fluoro-d-glucose are poor substrates and have very similar K(m) values (8x10(-2)m). 3. Neither alpha- nor beta-d-glucopyranosyl fluoride is a substrate or inhibitor. 4. Studies with 2-chloro-2-deoxy- and 2-O-methyl derivatives of d-glucose and d-mannose have revealed that little chemical modification is possible at position 2 without substantial loss in substrate binding. 5. The variation in the value of K(m) for the d-hexose derivatives was associated with a corresponding change in the value of K(m) for MgATP(2-) showing that the binding of MgATP(2-) is modified by the binding of the sugar.


Radiochemical determination of a unique sequence around the reactive serine residue of a di-isopropyl phosphorofluoridate-sensitive plant carboxypeptidase and a yeast peptidase. Phaseolain, a carboxypeptidase from French-bean leaves, and a partially purified peptidase from baker's yeast are inhibited by reaction with di-isopropyl phosphorofluoridate. Radioactive di-isopropyl [(32)P]phosphorofluoridate was used to show that the site of reaction is a unique serine residue and that the sequence of amino acids adjacent to the reactive serine is Glu-Ser-Tyr. This sequence is different from those of other ;serine' enzymes previously reported and, for phaseolain, represents an unequivocal example of a ;serine' carboxypeptidase.


Reaction of dihydropyrrolizines with deoxyribonucleic acids in vitro. 1. Thermal denaturation profiles of Escherichia coli DNA pretreated with monocrotaline pyrrole in vitro showed no difference from control DNA samples during heating. A substantial increase in the degree of renaturation during cooling of the pretreated DNA samples was observed. The degree of renaturation was dependent on the concentration of pyrrole used. 2. When rat liver DNA was pretreated with monocrotaline pyrrole there was a greater degree of renaturation after heat treatment than was found with E. coli DNA. 3. Equilibrium-density-gradient centrifugation in alkaline caesium chloride showed that DNA pretreated with monocrotaline pyrrole and heat denatured, renatured to a greater extent on quenching in ice than did untreated control DNA. The degree of renaturation was similar whether the initial treatment of the DNA with pyrrole was for 1 or for 15min. The reaction also appeared to be independent of pH between 5.5 and 9.0. 4. Retrorsine pyrrole was as effective as monocrotaline pyrrole in cross-linking the DNA, but monocrotaline pyrrole exposed to water for 5min before the addition of the DNA was ineffective. Pretreatment of E. coli DNA with synthetic bis-hydroxymethylpyrrole esters also caused renaturation after heat treatment. Monoesters were ineffective. 5. After treatment of rats with retrorsine, no cross-linking of the liver DNA could be demonstrated.


The purification and properties of Escherichia coli methylglyoxal synthase. 1. Methylglyoxal synthase was purified over 1500-fold from glycerol-grown Escherichia coli K 12 strain CA 244. The purified enzyme was inactivated by heat or proteolysis, had a molecular weight of approx. 67000, a pH optimum of 7.5 and was specific for dihydroxyacetone phosphate with K(m) 0.47mm. 2. The possibility that a Schiff-base intermediate was involved in the reaction mechanism was investigated but not confirmed. 3. The purified enzyme lost activity, especially at low temperature, but could be stabilized by P(i). Two binding sites for P(i) may be present on the enzyme. Of other compounds tested only the substrate, dihydroxyacetone phosphate, and bovine serum albumin showed any significant stabilizing effect. 4. Phosphoenolpyruvate, 3-phosphoglycerate, PP(i) and P(i) were potent inhibitors of the enzyme. Kinetic experiments showed that PP(i) was apparently a simple competitive inhibitor, but inhibition by the other compounds was more complex. In the presence of P(i) the enzyme behaved co-operatively, with at least three binding sites for dihydroxyacetone phosphate. 5. It is proposed that methylglyoxal synthase and glyceraldehyde 3-phosphate dehydrogenase play important roles in the catabolism of the triose phosphates in E. coli. Channelling of dihydroxyacetone phosphate via methylglyoxal would not be linked to ATP formation and could be involved in the uncoupling of catabolism and anabolism.


Effects of emetine and cycloheximide on mitochondrial protein synthesis in different systems.


Wheat-germ aspartate transcarbamoylase. Kinetic behaviour suggesting an allosteric mechanism of regulation. 1. Some kinetic properties of aspartate transcarbamoylase (EC 2.1.3.2), that had been purified approx. 20-fold from wheat germ, were studied. 2. A plot of enzyme activity against pH showed a low maximum at pH8.4 and a second, higher, maximum at pH10.5. A plot of percentage inhibition by 0.2mm-UMP against pH was approximately parallel to the plot of activity against pH, except that between pH6.5 and 7.5 the enzyme was insensitive to 0.2mm-UMP. 3. Kinetics were studied in detail at pH10.0 and 25 degrees C. In the absence of UMP, initial-rate plots were hyperbolic when the concentration of either substrate was varied. UMP decreased both V(max.) and K(m) in plots of initial rate against l-aspartate concentration, but the plots remained hyperbolic. However, UMP converted plots of initial rate against carbamoyl phosphate concentration into a sigmoidal shape, without significantly affecting V(max.). Plots of initial rate against UMP concentration were also sigmoidal. 4. The theoretical model proposed by Monod et al. (1965) gave a partial explanation of these results. When quasi-equilibrium conditions were assumed analysis in terms of this model suggested a trimeric enzyme binding the allosteric ligands, carbamoyl phosphate and UMP, nearly exclusively to the R and T conformational states respectively, and existing predominantly in the R state when ligands were absent. However, the values of the Hill coefficients for the co-operativity of each allosteric ligand were somewhat less than those predicted by the theory. 5. Some of the implications of these results are discussed, and the enzyme is contrasted with the well-known aspartate transcarbamoylase of Escherichia coli.


The pH dependence and quenching of the fluorescence of asparaginase from Escherichia coli B.


William Alexander Bain, Ph.D., D.Sc., F.R.S.E.


Inhibitory and stimulatory effects of secretin on gastric secretion in conscious rats. 1. The effect of pure secretin on gastrin-induced secretion has been studied in conscious rats with Pavlov or Heidenhain pouches.2. Rapid intravenous injection of secretin did not inhibit a plateau secretion of acid in the Heidenhain pouch, but infusion of the same amount of secretin over two hours inhibited secretion in both Heidenhain and Pavlov pouches.3. A rapid intravenous injection of secretin before the onset of gastrin infusion enhanced the acid secretory response to gastrin in both types of pouches.4. Secretin stimulated pepsin secretion.


In situ renal perfusion.

In situ renal perfusion.


Effect of heptachlor and related compounds on growth of Staphylococcus aureus.


Muscular dystrophies.

Muscular dystrophies.


Gamma ray mutagenesis in a strain of Escherichia coli deficient in DNA polymerase I.


Asbestos-fibre concentration in relation to the pulmonary reaction.


A comparison of pig lymph-node reactions to DNCB and skin homografts.


Virus-specific immunoglobulins in sera and cerebrospinal fluid in multiple sclerosis.


Occupational aspects of Raynaud's disease: a critical historical survey.


Computer aided diagnosis: a study of bronchitis.


Virulent Escherichia coli from a mouse.

Virulent Escherichia coli from a mouse.


Epistatic interactions between four rad loci in yeast.


The variation in photoreactivating enzyme activity as a function of the stage of growth of three K12 strains of Escherichia coli.


On the mutagenic action of dichlorvos.

On the mutagenic action of dichlorvos.


Enzyme replacement therapy by renal allotransplantation in Fabry's disease.


Pancreatic abscesses.

Pancreatic abscesses.


Mazindol, a new non-amphetamine anorexigenic agent.


A pilot study of the short-term antianxiety effects of molindone HCl.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 50-1972.


Henry J. Bakst, M.D.

Henry J. Bakst, M.D.


Enzyme transplantation in Fabry's disease.


Treatment of oxaluric renal failure.

Treatment of oxaluric renal failure.


The dramatic future of the polymers in dentistry.


Effect of autoimmunization with irradiated tumour cells on specific cell-mediated immunity in patients with malignant melanoma.


The compton metabolic profile test.

The compton metabolic profile test.


The development of the hospitals in Brighton and Hove.


Gideon Algernon Mantell LLD FRCS FRS (1790-1852). Surgeon and geologist: "Wizard of the Weald".


Patterns, causes and prevention of facial injury in car occupants.


Soft-tissue aspects of facial fractures.


Principles and techniques of autogenous bone grafting to the mandible.


Hydrophilic contact lenses for correcting irregular and high astigmatism.


Strabismus.

Strabismus.


"Infection control".

"Infection control".


Immunofluorescence studies on immunoglobulins in the lymphoid cells of human peripheral blood.


A new approach for determining the posterior limit of the upper denture for post-damming.


Survey of prosthetic technology in commercial dental laboratories.


Lymphocytes with membrane-bound immunoglobulin (B-lymphocytes) in new-born babies.


Modulation of the immune response to transplantation antigens. I. The effects of immunization using adjuvants on the immune response of mice to a tumour allograft.


Illinois State Dental Society membership directory addendum.


Considerations in partial denture failure.


Immunoglobulin-like surface molecules and theta antigen during the specific and non-specific stimulation of mouse spleen cells in vitro.


Run off ribosomes of Escherichia coli: their accumulation in response to cold treatment and their dissociation during centrifugation.


The woman alcoholic. A review of the literature.


Inhibition by progesterone of IUD-induced luteal regression in the ewe. A U.S. Department of Agriculture study at Beltsville, Maryland investigated the effect of progesterone on IUD-induced luteal regression in 95 ewes.  The IUD was a plastic spiral with diameter 9 mm and length 30 mm.  The first experiment suggested that IUD-induced regression of a corpus luteum (CL) is dependent upon the stage of the estrous cycle but unrelated to the age of the CL.  When ovulation was induced on Cycle Day 5 in 4 ewes and an IUD was inserted on Day 7, neither the original CLs, about 6 days old, nor the induced CLs, about 1 day old, regressed.  Experiment 2 provided evidence that exogenous progesterone generally prevents IUD-induced luteal regression.  29 ewes were injected twice daily on Days 1-4 of a cycle with 10 mg of progesterone in corn oil or with corn oil alone.  An IUD was inserted on Day 3.  Sacrifice was on Day 9.  Early CL (ovaries ipsilateral to IUD) regression occurred in 7 in the corn-oil group but in only 2 in the progesterone group.  In Experiment 3 the administration of progesterone to estradiol- and IUD-treated ewes tended to reduce the percentage of ewes with regressed CL's (p=.10, ovaries ipsilateral to IUD).  Estradiol itself extends the stage during which the insertion of an IUD causes luteolysis.


Stimulation by sulfhydryl-reactive reagents of peptidyl transferase and donor site binding activities in a reticulocyte system.


Membership roster--Oklahoma State Dental Association.


Effects of solvents and irritants on intraocular tension in the rabbit.


A modified headholder for acute stereotaxic experiments on rabbits.


Synaptic actions of peripheral nerve impulses upon Deiters neurones via the climbing fibre afferents. 1. The cerebellar integration of sensory inputs to Deiters neurones was studied in cats under Nembutal anaesthesia.2. Stimulation of peripheral nerves produced in the Deiters neurones a sequence of an initial excitatory post-synaptic potential (e.p.s.p.) and a later inhibitory post-synaptic potential (i.p.s.p.), or a relatively small e.p.s.p.3. The Deiters neurones were classified as forelimb (FL)- or hind limb (HL)-type cells according to the location of the most effective peripheral nerve. In the FL cells stimulation of the forelimb nerves produced the e.p.s.p.-i.p.s.p. sequence (dominant response), while stimulation of the hind limb nerves was ineffective or produced the small e.p.s.p. (non-dominant response). In contrast, in the HL cells the non-dominant response was evoked from the forelimb nerves, and the dominant response from the hind limb nerves.4. The stimulus intensity-response relation indicates that Group I and II muscle afferents and low and high threshold cutaneous afferents contribute to the dominant and non-dominant responses.5. Antidromic identification of these Deiters neurones revealed that 90% of the HL cells and 85% of the FL cells project to the lumbo-sacral and cervico-thoracic segments of the spinal cord, respectively, while 10% of the HL cells and 15% of the FL cells innervate the cervico-thoracic and lumbo-sacral segments, respectively.6. The mean latency of the e.p.s.p. evoked from the forelimb nerves was 14 msec in the FL cells and 13 msec in the HL cells, and the latency of the e.p.s.p. evoked from the hind limb nerves was 17 msec in the FL cells and 18 msec in the HL cells. The later i.p.s.p. regularly followed the onset of the e.p.s.p. with a delay of 3-5 msec.7. The dominant and non-dominant responses in both types of cells exhibited the following three characteristic features: (i) a strong depression after conditioning stimulation of the inferior olive, (ii) an increase of the inferior olivary excitability during the responses, and (iii) a striking frequency depression with stimulation at relatively low frequency (5-10/sec).8. Consequently it was concluded that all of the responses were produced through the climbing fibres originating from the inferior olive, the i.p.s.p.s due to inhibition from Purkyne cells activated by the climbing fibres and the e.p.s.p.s due to excitation from the collaterals of the climbing fibres.


Synaptic actions of peripheral nerve impulses upon Deiters neurones via the mossy fibre afferents. 1. The cerebellar integration of sensory inputs to Deiters neurones was investigated in decerebrate cats. In some preparations decerebration was combined with transection of the olivocerebellar fibres.2. In the latter preparations peripheral nerve impulses generally produced a response consisting of a sequence of the following post-synaptic potentials: (i) an initial e.p.s.p. (d(1)), (ii) early i.p.s.p. (h(1)), (iii) later i.p.s.p. (h(2)).3. The mean latencies of d(1), h(1) and h(2) were 5.7, 7.3 and 9.8 msec from the forelimb nerves, and 7.5, 9.0 and 13.4 msec from the hind limb nerves, respectively.4. The stimulus intensity-response relation indicates that the Group I muscle afferents as well as the low threshold cutaneous afferents contribute to the response.5. In the preparations with the intact inferior olive there were additional components of the post-synaptic potentials: a later e.p.s.p. (d(2)) and another later i.p.s.p. (h(3)), their mean latencies being 15.3 and 19.7 msec from the forelimb nerves, and 18.0 and 21.3 msec from the hind limb nerves, respectively.6. The d(1) and h(2) components were attributed to the mossy fibre afferents and d(2) and h(3) to the climbing fibres; d(1) and d(2) were due to excitation through the collaterals of the mossy and climbing fibres, and h(2) and h(3) to inhibition from Purkyne cells activated by the mossy and climbing fibres, respectively. h(1) was too early to be produced through the cerebellum, and was probably mediated by inhibitory neurones in the reticular formation.


Demonstration of the movement of histocompatibility antigen and immunoglobulin on the pig lymphocyte membrane by immunofluorescence.


The faggot method: a technique for the separation of blood lymphocytes.


The effects of a synthetic decapeptide on the release of luteinizing hormone and follicle-stimulating hormone from ovine pituitary tissue in vitro.


Gastric secretion and its facilitation as related to gastric mucosal histamine. 1. In conscious rats provided with Pavlov or Heidenhain pouches the acid and pepsin responses to vagal stimulation, or infusions of gastrin, histamine, methacholine, and to various combinations of these stimulants have been established. The concomitant mobilization and increased formation of gastric mucosal histamine have also been examined.2. Histamine evoked graded acid responses in the Pavlov and Heidenhain pouches. The innervated pouch was more sensitive to histamine than the denervated. Stimulation of pepsin secretion was apparent only in the Heidenhain pouch.3. The acid response to vagal stimulation evoked by 2-deoxy-D-glucose was facilitated by infusion of histamine, but not that of pepsin, the secretion of which was depressed.4. Acid secretion in response to graded infusion of methacholine was enhanced by background infusions of histamine in subthreshold, sub-maximal and maximal dosages.5. The acid response to vagal stimulation was enhanced by a background infusion of gastrin, but not that of pepsin, the secretion of which was depressed.6. A background infusion of a large dose of histamine depressed the maximal acid response to gastrin in both types of pouches.7. Administration of histamine restrained the accelerating effect of gastrin on histidine decarboxylase activity, probably by a feed-back coupling.8. The increase in gastric mucosal histamine, as reflected in its urinary excretion, in response to gastrin infusion was enhanced by vagal stimulation.9. The amounts of histamine that can be mobilized and newly formed in the gastric mucosa have been evaluated and found to be fully adequate for efficient stimulation of the parietal cell.


Stress analysis related to tooth preparation and fixed partial denture design.


Members of Indiana Dental Association, 1972.


Hints on suturing an episiotomy and perineal tears by midwives in remote areas.


Structure-activity relationship of mono- and bisquaternary pyridines in regard to their parasympatholytic effects.


Meckel's diverticulum.

Meckel's diverticulum.


Infant mastitis due to gram-negative organisms.


Gastroscopy and photography are not enough. The importance of cytologic and histologic diagnosis in gastric ulcer.


Frostbite in a pre-Columbian mummy.

Frostbite in a pre-Columbian mummy.


Diet and attrition in the Natufians.

Diet and attrition in the Natufians.


Histological examination of experimentally mummified tissues.


Ultralow volume (ULV) application of insecticides.


1972 Kruzen Award (Frank H. Kruzen, M.D.).


1972 Gold Key Award (William A. Spencer, M.D.).


New academy president (Justus F. Lehmann, M.D.).


Anomalies of the small acrocentric chromosomes in human tumour cells.


The chemistry and biochemistry of polyprenyl alcohols such as solanesol and dolichol.


The role of intracellular pH in the regulation of cation exchanges in yeast. 1. When yeast oxidizes propan-2-ol in the presence of KCl no uptake of K(+) occurs. 2. When propionate is added to suspensions containing propan-2-ol, or if the suspensions are bubbled with CO(2), a considerable uptake of K(+) occurs. 3. Maximum K(+) uptake occurs at a propionate concentration of 2mm. 4. The addition of 20mm-propionate to the suspension lowers the intracellular pH of the yeast from a resting value in the region of 6.2 to approx. 5.6. 5. When K(+) uptake is measured in the presence of 20mm-propionate, progressive changes in the rate of K(+) uptake and intracellular pH occur. The optimum rate of K(+) uptake occurs at an intracellular pH of 5.70. 6. The effect of both intra- and extra-cellular pH on K(+)-K(+) exchange was studied and an optimum rate was found at an extracellular pH of 5.35, the corresponding intracellular pH being 6.44. 7. When a Na(+)-loaded yeast oxidizes propan-2-ol in the presence of KCl, a steady efflux of Na(+) and influx of K(+) occurs. The addition of 10mm-propionate to the suspension markedly inhibited the Na(+) efflux but only slightly decreased the K(+) influx. 8. The effect of both extra- and intra-cellular pH on Na(+) efflux was studied with propan-2-ol and with glucose. The results can be best interpreted in terms of intracellular pH changes, and an optimum was obtained at approx. pH6.40.


The molecular organization of lipids in the membrane of Escherichia coli.


Regulation of phosphoglucomutase.

Regulation of phosphoglucomutase.


Decrease of the bacterial content in different types of oxidation ponds.


Mechanism of malonyl-coenzyme A-acyl-carrier protein transacylase.


Do phospholipases cause the breakdown of mitochondrial membrane?


Synthesis of the late proteins of bacteriophage T 4  amber mutant.


Observations on the mode of action of the polyene antibiotic A-435.


The metabolic factors limiting the growth of Saccharomyces cerevisiae subjected to  -irradiation.


Thymine starvation and deoxyribonucleic acid synthesis in Escherichia coli.


Incorporation of cis-hydroxyproline into collagen by tendon cells. Failure of the intracellular collagen to assume a triple-helical conformation.


Obituary. Lothair Henry Rubinstein.

Obituary. Lothair Henry Rubinstein.


Evidence for the limited spread of intracerebrally applied serotonin.


Electron microscope investigations of the structure of some specimens of the Trypanosomidae family in connection with their ability to occur in the dyskinetoplastic state. 1. Trypanosoma.


Studies of clinical judgment. II. Toward a new model for research.


Practical use of penicillin, cephalosporin and aminoglycoside antibiotics.


A permanent change in convulsive threshold in normal and brain-damaged rats with repeated small doses of pentylenetetrazol.


Synthesis and pharmacological activity of some chlorphenesin phenoxyalkanoates.


Brain of the buffalo (Bubalus bubalis) in stereotaxic coordinates.


Robert Boyle's poem--an addendum.

Robert Boyle's poem--an addendum.


Enhanced hemolytic antibody response following thermal injury.


Ground itch and dew poison; the Rockefeller Sanitary Commission 1909-14.


Caesarean operation from the Armamentarium chirurgicum by J. Scultetus, 1666, engraved by Jonas Arnold.


Mechanisms of cell and tissue injury induced by group A streptococci: relation to poststreptococcal sequelae.


Early descriptions of the Arnold-Chiari malformation. The contribution of John Cleland.


Variation in LH and FSH response to LH-releasing hormone during the menstrual cycle.


On the pathogenesis of pre-eclampsia and eclampsia.


The effect of bromelain therapy on episiotomy wounds--a double blind controlled clinical trial.


Dementia in adults.

Dementia in adults.


The role of platelets in glomerulonephritis and transplantation.


Aspects of immunology in radiotherapy.

Aspects of immunology in radiotherapy.


Extracorporeal irradiation of the blood.


The suggestion of George Bernard Shaw. An adjunct in men's facelifting.


Histopathological techniques in the investigation of muscle disease.


Compound fractures of the tibia: the soft-tissue defect.


An immunological approach to the detection of neoplasia.


A review of studies of breast cancer in human populations excluding endocrine studies.


Medicine and the 1851 exhibition.

Medicine and the 1851 exhibition.


Endocrine, genetic and viral factors in the etiology of breast cancer.


Significance of mercury in the environment.


Primary structure of neocarzinostatin, an antitumor protein. The antitumor protein neocarzinostatin is an acidic single-chain molecule, cross-linked by two disulfide bridges, and consists of 109 amino acid residues. Complete disulfide bond reduction and S-carboxymethylation was achieved in liquid ammonia. Sequence determination of five tryptic fragments led to the proposed primary structure.


Medicinal chemistry of tetrahydrothiophenes and tetrahydrothiopyrans.


Birth order: a critical review.

Birth order: a critical review.


An experimental study into the validity of clearance methods of measuring renal blood flow.


The hemodynamics of the axillary-axillary bypass.


Frozen section experience in 3,249 specimens.


Plasma and erythrocyte concentrations of sodium and potassium during hemodialysis in anephric patients.


Aortocoronary bypass vein preparation.

Aortocoronary bypass vein preparation.


The endocrine pancreas after kwashiorkor.


Effect of steroid contraceptives on lactation.


Isoenzymes and ontogeny.

Isoenzymes and ontogeny.


Neurobiology of echinodermata.

Neurobiology of echinodermata.


Rapid characterization of bacteria, with emphasis on Staphylococcus aureus.


The control of haemoglobin synthesis.

The control of haemoglobin synthesis.


The nomenclature of Salmonella, its usage, and definitions for the three species.


Effect of temperature on the cellular integrity of Bacillus psychrophilus.


An S-adenosylmethionine requirement for chemotaxis in Escherichia coli.


Glycerol transport by Nocardia asteroides.


Variability in ultrastructure of Clostridium botulinum spores.


Studies on the bacterial flora of vacuum-packaged fresh beef.


The toxic effect of 4-chloro-2-cyclopentylphenyl  -D-galactopyranoside in lactose broth on the growth of Shigella in mixed culture with Escherichia coli.


Communal growth response of a heat-injured strain of Escherichia coli.


Candida iberica sp. n. A new species isolated from spanish sausages.


A low resolution electron-density map of lipoyl transsuccinylase, the core of the  -ketoglutarate dehydrogenase complex.


Structure-reactivity correlations and general acid-base catalysis in enzymic transacylation reactions.


Spectro-chemical probes for protein conformation and function.


Enzymatic prerequisites for ATP formation from triose phosphates in the A 2  and B-cells of the endocrine pancreas of the guinea-pig.


Addendum to "a review on mathematical models of the human thermal system".


John Joseph Cronin Buckley, D.Sc. 1904-1972.


Fine structure of goldfish xanthophore.

Fine structure of goldfish xanthophore.


A simple efficient procedure for the preparation of fragments corresponding to the variable-half and constant-half portions of Bence Jones protein.


The rise and fall of antibody avidity at the level of single immunocytes.


The generation of antigen sensitive cells in the newborn mouse.


Blastogenic factor from unstimulated leukocyte cultures: further evidence for heterogeneity, and mechanism of production.


American species of Strychnos.

American species of Strychnos.


Peter Gerald Jay.

Peter Gerald Jay.


A record of nursing: Boston University Nursing Archive.


Thiamine intestinal transport.

Thiamine intestinal transport.


A Japanese interpretation of Erich Segal's Love Story.


Prolonged survival of second human kidney transplants. Rejection of kidney transplants in 264 patients, followed by retransplantation from cadaver donors, resulted in a 1-year survival rate of 51 +/- 3 percent (rate +/- standard error) as compared to 51 +/- 1 percent for first transplants. If the first transplant immunizes the patient or is rejected by immunologically responsive patients, second grafts into the same patients would be expected to be rejected at a higher rate. Only those reject who reject first grafts hyperacutely or between 1 to 3 months were found to have low second graft survival rates. Patients who rejected transplants after 3 months tended to have second transplant survival rates which were higher than their first graft survival rates.


The digitalis glycosides: contractile and electrophysiologic actions, clinical indications, precautions, and toxicity.


Treatment of the failing heart independent of digitalis or its glycosides.


Punishment of human behavior.

Punishment of human behavior.


Neonatal hypoglycaemia--a critical reappraisal.


Regulation of erythropoiesis in the fetus and newborn.


Gastric aspirate findings in neonatal pneumonia.


Leucocyte blood picture in ill newborn babies.


Artificial grunting in respiratory distress syndrome.


Purification and properties of ribosome-releasing factor.


Fractionation of nucleic acids by isoelectric focusing.


Specificity of the neurotoxin from Lathyrus sativus as an amino acid antagonist.


Active vs. passive sensitization of liposomes toward antibody and complement by dinitrophenylated derivatives of phosphatidylethanolamine.


Thermodynamic studies of transfer ribonucleic acids. 3. Thermodynamic model for the thermal unfolding of yeast phenylalanine-specific transfer ribonucleic acid.


Studies on the specificity of chymosin (rennin). I. Kinetic parameters of the hydrolysis of synthetic oligopeptide substrates.


Ribosomal proteins. XXXII. Comparison of several extraction methods for proteins from Escherichia coli ribosomes.


Insertion mutations in microorganisms.

Insertion mutations in microorganisms.


Biogel-absorbed insoluble protein derivatives for the isolation of antibody.


Characterization of messenger RNA from embryonic muscle.


Prof. Dr. H. C. Huizing 1903-1972.

Prof. Dr. H. C. Huizing 1903-1972.


James Bell's appendicitis.

James Bell's appendicitis.


Clinical trial of a new absorbable synthetic suture material: polyglycolic acid.


Reversal of skeletal changes in renal osteodystrophy following partial parathyroidectomy and renal allotransplantation. A case report.


Footgear--its history, uses and abuses.

Footgear--its history, uses and abuses.


Adduction--flexion contracture of the thumb. Correction with dorsal rotation flap and release of contracture.


The biological response to zinc polyacrylate cement.


British orthopaedic surgery.

British orthopaedic surgery.


Spine ostectomy for kyphosis in myelomeningocele.


The classic. The effects of artificial electricity on muscular motion. Aloysio Luigi Galvani.


Orthopaedics in ancient Greece.

Orthopaedics in ancient Greece.


Fine structure of the endocrine pancreas in newborn rodents.


Structural and enzymic studies on the process of dye-sensitized photoinactivation of yeast hexokinase.


Association of ribosomal subunits: mechanism of the reaction induced by association factor.


Methionine-selenomethionine parallels in rat liver polypeptide chain synthesis.


Stimulation of in vitro transcription by ribonuclease H (hybridase).


Prof. Raymond Garcin. (1897-1971).

Prof. Raymond Garcin. (1897-1971).


Characteristics of two gold alloys used in fabrication of porcelain-fused-to-metal restorations.


Dental management of the patient treated by renal transplantation: preoperative and postoperative considerations.


Alcohol-induced thrombocytopenia: review of the literature and report of case.


"Induction" of colicin factor E2-P9 by mitomycin C. Mitomycin C increases the titers of colicin E2-P9 without increasing the amount of colicin factor deoxyribonucleic acid.


Purine phosphoribosyltransferases of Salmonella typhimurium. Evidence is presented that Salmonella typhimurium contains two guanine phosphoribosyltransferases.


Nutritional requirements of Acinetobacter strains isolated from soil, water, and sewage. One hundred five strains of Acinetobacter were isolated from water, soil, and sewage on nonselective complex media, and their nutritional properties were studied. Only one of these strains requires growth factors in order to grow in a mineral medium containing a single carbon source.


Host cell deoxyribonucleic acid polymerase I and the support of T4 bacteriophage growth. Ultraviolet irradiation of Escherichia coli polA(-) cells reduces their capacity to support the growth of T4 phage. There is no additional loss of capacity observed in pol tsA(-)recA(-) double mutants at the nonpermissive temperature. The reversion frequency of a T4 rII mutant after ultraviolet irradiation is not changed by the absence of host deoxyribonucleic acid polymerase I.


Recombination among three mitochondrial genes in yeast (Saccharomyces cerevisiae). Eight crosses involving three different mitochondrial genes were made in Saccharomyces cerevisiae. All three genes were linked, but an unambiguous linkage map could not be constructed.


Conditional lethality of deletions which include uvrB in strains of Escherichia coli lacking deoxyribonucleic acid polymerase I. Deletions of the uvrB gene were not obtained in polA1 strains of Escherichia coli either by selecting for spontaneous deletions or by transduction from strains carrying such deletions. A strain forming a temperature-sensitive deoxyribonucleic acid polymerase I and carrying a deletion of the uvrB gene is inviable at the nonpermissive temperature.


Temperature-sensitive osmotic remedial mutants of Escherichia coli. A collection of temperature-sensitive mutants of Escherichia coli K-12 was examined for ability to grow at the restrictive temperature when the osmotic pressure of the medium was increased. Five of the fourteen mutants were found to be osmotic remedial. Four strains containing temperature-sensitive, osmotic-remedial mutations affecting aminoacyl-transfer ribonucleic acid synthetases were found to have altered permeability characteristics which may be attributable to changes in the lipopolysaccharide layer of the cell envelope at restrictive temperatures.


Self-transfer and genetic recombination mediated by P, the sex factor of Vibrio cholerae. Vibrio cholerae cells, infected with the sex factor P, produce discrete, plaque-like clearings when plated on lawns of P(-) cells. We investigated the nature of these clearings and conclude that they are probably sites of active mating. We developed a quantitative assay for P(+) cells and used it to study the kinetics of sex factor spread in broth cultures. Both established and newly infected donor populations were efficient sex factor donors, indicating that P is not self-repressed. We also investigated the kinetics of recombinant formation in broth matings. In 1-hr matings, we routinely found recombination frequencies of 10(-6) per donor cell. Kinetic studies of recombinant formation showed that the markers tested all appeared at early times. Thus P, the V. cholerae sex factor, seems to resemble F in its transfer properties.


Isolation and characterization of a mutant colicin E2. Escherichia coli K-12 colicinogenic for Col E2 yielded a mutant, SK95, that carries a nonsense mutation in the colicin structural gene. A derivative of SK95 that carries an as yet unidentified suppressor mutation produces a colicin E2 that is temperature sensitive (TS). This mutant colicin kills sensitive cells at low temperature but not at high temperature; the colicin adsorbs to cells at high temperature but does not kill them unless the temperature is lowered. Unlike normal colicin E2, which adsorbs rapidly to cells, TS colicin E2 adsorbs slowly over a period of several hours. The biochemical target of colicin E2 is deoxyribonucleic acid (DNA). When acid solubilization of DNA was compared in cells treated with either TS or normal colicin E2, striking differences were observed. Cell killing and acid solubilization of DNA by colicin E2 were shown to be separable events under certain conditions. The results are discussed in relation to the mechanism of action of colicin E2.


Homology between the deoxyribonucleic acids of Haemophilus influenzae and Haemophilus parainfluenzae. To determine the degree of homology between deoxyribonucleic acid (DNA) from Haemophilus influenzae and that from Haemophilus parainfluenzae, the two DNAs were hybridized by the membrane-filter technique. It was found that 44% of the DNA from each species was sufficiently homologous to allow hybrid formation.


Isoleucine and threonine can prolong protein and ribonucleic acid synthesis in pyridoxine-starved mutants of Escherichia coli B. Pyridoxineless mutants of Escherichia coli B stopped incorporation of nucleosides into trichloroacetic acid-insoluble material about 40 to 60 min after pyridoxine starvation was initiated, whereas incorporation of amino acids (measured the same way) slowed but did not stop for several hours. Both these incorporations and cell density were increased most effectively by the presence of either threonine or isoleucine. Arginine, glutamate, histidine, methionine, tryptophan, and tyrosine also caused significant but less dramatic increases. Inducibility of beta-galactosidase continued beyond the point where nucleic acids appeared to stop their synthesis, suggesting that messenger ribonucleic acid synthesis continued beyond ribosomal ribonucleic acid synthesis. This inducibility was also increased by isoleucine and threonine. The overall results suggest that the threonine-isoleucine biosynthetic pathway is the most sensitive to starvation for pyridoxine.


Temperature-dependent utilization of meso-inositol: a useful biotyping marker in the genealogy of Salmonella typhimurium. Salmonella typhimurium strains from natural sources either ferment or do not ferment meso-inositol in peptone water in 24 hr at 37 C. Ninety-five percent of the strains that are designated inositol-nonfermenting on the basis of their phenotype at 37 C ferment inositol when incubated at 25 C. Two classes of temperature-sensitive mutants were detected among the 712 strains of S. typhimurium examined. The occurrence of low-temperature fermentation of inositol among wild-type strains of S. typhimurium from biotypes 10 through 13 and FIRN suggested a genealogical relationship between these two groups, and that FIRN strains (fim(-)inl(ts)rha(-)) might have descended from ancestral types like biotype 10 through 13 strains (fim(+)inl(ts)rha(+)).


Importance of facilitated diffusion for effective utilization of glycerol by Escherichia coli. Wild-type Escherichia coli possesses an inducible permeation system which catalyzes facilitated diffusion of glycerol into the cell. A spectrophotometric method can be used to assess the presence of this mechanism. The structural gene for the facilitator (glpF) and the structural gene for glycerol kinase (glpK) apparently belong to a single operon. The glpF(+) allele permits effective glycerol utilization by the cells, and, at millimolar concentrations of glycerol, cells carrying the glpF(+) allele grow much faster than glpF genotypes. Although the glycerol-scavenging power of the cell depends both on the facilitated entry of the substrate and its subsequent trapping by an adenosine triphosphate-dependent phosphorylation, the two gene products, the facilitator and kinase, function independently. Wild-type Shigella flexneri appears to be glpK(+) but glpF. This organism grows slowly in media at low concentrations of glycerol. When the glpF(+) and glpK(+) alleles of E. coli are inserted into the S. flexneri genome by transduction, the hybrid strain grows rapidly in low glycerol medium. Vice versa, when the glpF and glpK(+) alleles of S. flexneri are incorporated into E. coli, the hybrid strain grows slowly in low glycerol medium.


Relationship between sporulation-specific 20S ribonucleic acid and ribosomal ribonucleic acid processing in Saccharomyces cerevisiae. The nature and properties of the 20S ribonucleic acid which accumulates only during the sporulation of Saccharomyces cerevisiae were examined. The 20S ribonucleic acid (RNA) has a base composition considerably different from ribosomal RNA species and is virtually unmethylated. The 20S RNA did, however, exhibit approximately 70% homology with 18S RNA by RNA-deoxyribonucleic acid filter hybridization competitions. The 20S RNA showed a hybridization saturation plateau level 30 to 40% higher than 18S, consistent with measurements of the size difference in polyacrylamide gels. Pulse-chase experiments in the presence and absence of cycloheximide indicate that the 20S RNA has a presumptive relationship to the 20S ribosomal RNA precursor normally observed only in short pulse-labeling in vegetative cells.


Deletion and complementation analysis of biotin gene cluster of Escherichia coli. Genetic deletions that terminate within the cluster of genes needed for biotin biosynthesis in Escherichia coli have been isolated and mapped by transduction with phages lambda and P1. These deletions order the point mutations in each of the five genes. Mutations causing biotin dependence were incorporated into lambdapbio transducing phages. New bio(-) mutations were induced by exposure of lambdapbio particles to ultraviolet light. Tests of complementation between such bio(-)pbio particles and bio(-) mutant cells divide the bio(-) mutations into five cistrons: bioA, bioB, bioF, bioC, and bioD. Certain bioA and bioF mutations exhibit intragenic complementation, suggesting that these genes determine enzymes composed of identical subunits.


Reversal of the mannitol-sorbitol diauxie in Escherichia coli. In Escherichia coli K-12 the proteins involved in the dissimilation of mannitol and sorbitol are specified by two separate gene clusters. The mannitol cluster appears to consist of a regulatory gene mtlC, a gene mtlA coding an enzyme II complex of the phosphoenolpyruvate phosphotransferase system, and a gene mtlD coding a mannitol-1-phosphate dehydrogenase. Three corresponding genes, sblC, sblA, and sblD, exist for the sorbitol pathway. In both pathways the hexitol captured from the medium and delivered into the cytoplasm as a phosphorylated compound is dehydrogenated to fructose-6-phosphate. The enzyme II complex for sorbitol is able to catalyze the phosphorylation also of mannitol if this substrate is present at high concentrations. Consequently mtlA(-) mutants lacking the enzyme II complex for mannitol can grow on mannitol either if the sorbitol phosphorylating system is preinduced by sorbitol or if mtlA is suppressed by a mutation of sblC to constitutivity. In wild-type cells, the induction of the enzymes in the mannitol pathway and dissimilation of the substrate are not prevented by glucose. The sorbitol system, however, is sensitive to glucose and to mannitol as well. In the suppressed strains (mtlA(-), sblC(c)) in which mannitol is utilized through the sorbitol enzyme, glucose becomes effective in restraining the consumption of mannitol, causing a definite diauxie. Moreover, in a mixture of mannitol and sorbitol, the latter is utilized preferentially. This reversal of normal diauxic pattern is consequent to the fact that the enzyme II complex for sorbitol has relatively poor affinity for mannitol.


High-level production of  -galactosidase by Escherichia coli merodiploids. Two merodiploids of Escherichia coli that contain genes for the lac operon on both chromosome and episome were tested for production of lac enzymes after growth on various carbon sources. The specific activity of beta-galactosidase (and of thiogalactoside transacetylase) was about twice that from haploid cells when grown on glycerol. With succinate as carbon source, the specific activity increased by an additional factor of 3. Up to 25% of the soluble cell protein is beta-galactosidase in these strains, one of which is inducible and the other constitutive. The enzyme is purified easily in high yield by ammonium sulfate fractionation and electrophoresis.


Molecular studies on entry exclusion in Escherichia coli minicells. Minicells produced by abnormal cell division in a strain of Escherichia coli (K-12) have been employed here to investigate the phenomenon of "entry exclusion." When purified minicells from strains containing F' or R factors, or both, are mated with radioactive thymidine-labeled Hfr or R(+) donors, the recipient minicells can be conveniently separated from normal-sized donors following mating, and the products of conjugation can be analyzed in the absence of donors and of further growth of the recipients. Transmissible plasmids or episomes are transferred less efficiently to purified minicells derived from strains carrying similar or related elements than to strains without them. Measurement of deoxyribonucleic acid (DNA) degradation and determination of weight-average molecular weights following transfer indicate that degradation of transferred DNA or transfer of smaller pieces cannot account for the comparative reduction in transfer to entry-excluding recipients. Therefore, we conclude that entry exclusion operates to prevent the physical entry of DNA into recipients expressing the exclusion phenotype. The R-produced repressor (product of the drd(+) gene), which represses fertility (i.e., ability to act as donor), reduces exclusion mediated by R or F factor, or both, in matings between strains carrying homologous elements. Furthermore, the data suggest that the presence of the F pilus or F-like R pilus on recipient cells ensures maximum expression of the exclusion phenotype but is not essential for its expression. In contrast to previous suggestions, we found no evidence for a reduction of entry exclusion attributable to the DNA temperature-sensitive chromosomal mutation dnaB(TS).


Restoration of phosphoribosyl transferase activity by partially deleting the trpB gene in the tryptophan operon of Salmonella typhimurium. The amber mutant trpA28, which contains a mutation mapping within the so-called "unusual" region of the tryptophan (trp) operon of Salmonella typhimurium (between the genes trpA and trpB), lacks both components of the anthranilate synthetase (AS)-phosphoribosyl transferase (PRT) enzyme complex, the products of the genes trpA and trpB, respectively. Twenty-six revertants of this mutant selected on minimal medium supplemented with anthranilic acid, a substrate of PRT, contain deletions of various segments of the "unusual" region and make a species of PRT different in every respect from the wild-type, dissociated form of this enzyme. The results indicate that the unusual region corresponds to the operator proximal end of the trpB gene. Mutants in the unusual region, however, show unexpectedly low levels of AS activity and in two cases (trpA515 and trpA28) no detectable activity of this enzyme component.


Amino sugar assimilation by Escherichia coli. The carbon skeleton of glucose is extensively randomized during conversion to cell wall glucosamine by Escherichia coli K-12. Exogenous glucosamine-1-(14)C is selectively oxidized, and isotope incorporation into cellular glucosamine is greatly diluted during assimilation. A mutant unable to grow with N-acetylglucosamine as a carbon and energy source was isolated from E. coli K-12. This mutant was found to be defective in glucosamine-6-phosphate deaminase. Glucosamine-1-(14)C and N-acetylglucosamine-1-(14)C were assimilated during the growth of mutant cultures without degradation or carbon randomization. Assimilated isotopic carbon resided entirely in cell wall glucosamine and muramic acid. Some isotope dilution occurred from biosynthesis, but at high concentrations (0.2 mm) of added N-acetylglucosamine nearly all cellular amino sugar was derived from the exogenous source. Growth of the mutant was inhibited with 1 mmN-acetylglucosamine.


Genetic separation of hypoxanthine and guanine-xanthine phosphoribosyltransferase activities by deletion mutations in Salmonella typhimurium. Certain proAB deletion mutants of Salmonella typhimurium were found to be simultaneously deleted in a gene required for the utilization of guanine and xanthine (designated gxu). These mutants were resistant to 8-azaguanine and when carrying an additional pur mutation were unable to use guanine or xanthine as a purine source. The defect was correlated with deficiencies in the uptake and phosphoribosyltransferase activities for guanine and xanthine. Hypoxanthine and adenine activities were unaltered. The deficiency was restored to normal by transduction to pro(+) and in F' merodiploids.


Interspecies transformation of Acinetobacter: genetic evidence for a ubiquitous genus. The availability of a strain of Acinetobacter competent for transformation has made it possible to demonstrate the genetic relatedness of a large variety of gram-negative, oxidase-negative, nonmotile, and aerobic coccobacilli originally classified into eleven different genera. Deoxyribonucleic acid (DNA) species from 265 such strains are capable of transforming stable auxotrophs of the competent Acinetobacter to prototrophy. The compositions of these DNA species vary from 40 to 46.8% guanine plus cytosine. Strains with widely differing phenotypic properties are also included in this collection of acinetobacters. DNA species from all oxidase-positive strains of Moraxella and from a variety of common bacteria are unable to transform the competent Acinetobacter. Although acinetobacters are usually considered to be unable to reduce nitrate to nitrite, six strains known to carry out this reduction have been shown to be authentic acinetobacters since their DNA species readily transform the competent Acinetobacter auxotrophs to prototrophy. In contrast to previous findings that acinetobacters rarely grow with glucose as a sole carbon source, the results of the present study show that 17 of the 265 strains grow readily in a glucosemineral medium, and 48 other strains can mutate spontaneously to grow in such a medium. A second competent strain of Acinetobacter, originally unable to use glucose, d-xylose, or d-ribose as carbon sources, has been transformed for ability to dissimilate these compounds using DNA species from strains that normally grow on these sugars. Although most of the 265 Acinetobacter strains studied were originally grown on complex media when isolated from human sources, only nine of these strains require growth factors in order to grow in a mineral medium containing a single carbon and energy source. A simple transformation assay has been devised for rapid examination of large numbers of strains to determine whether or not they are acinetobacters. This assay, which is suitable for routine diagnostic work, includes a procedure for preparation of crude transforming DNA from a small quantity of bacterial paste. Samples of DNA prepared from Acinetobacter cultures that had died on slants and plates were still able to effect transformation of the competent auxotrophs to prototrophy.


Cell wall peptidoglycan mutants of Escherichia coli K-12: existence of two clusters of genes, mra and mrb, for cell wall peptidoglycan biosynthesis. Temperature-sensitive mutants of Escherichia coli K-12 which cannot form cell wall peptidoglycan at 42 C were isolated. The thermosensitive steps were characterized biochemically, and the genes coding the enzymes of peptidoglycan synthesis were mapped. These genes were in two clusters: one group, located at about 1.5 min between leu and azi, was designated as mra (murein a); the second group, located at about 77.5 min close to argH and metB, was designated as mrb (murein b, with the order metB-argH-mrb). No simple relations were found between the gene location and the order or localization of enzymes involved in the sequence of reactions of cell wall peptidoglycan biosynthesis.


Rubin H. Flocks and the prostatic disease center.


Solution of the problem of energy coupling in terms of chemiosmotic theory.


Bioenergetics of nerve excitation.

Bioenergetics of nerve excitation.


Oxygen toxicity: basic and clinical considerations.


Rabid dogs in Bangkok--Thonburi. A preliminary study.


The American Board of Oral Surgery--a historical sketch.


Central hemangioma of the maxilla.

Central hemangioma of the maxilla.


Neonatal dermatology. 3. Pigmentary lesions and hemangiomas.


Review of thrombosis of abdominal aorta with report of two asymptomatic cases.


An address.

An address.


Isometric exercise and the cardiovascular system.


Experimental evidence of the flexibility of immunoglobulin G molecules.


Mistresses of the Royal Infirmary.

Mistresses of the Royal Infirmary.


Connective between parameters of fluorescence and protein structure.


Prematurity and uniqueness in scientific discovery.


Virginiamycin for prevention of swine dysentery.


Perioperative management of the infant with congenital heart disease.


Myocardial utilization of carbohydrate and lipids.


Computer in documentation.

Computer in documentation.


Some historical notes on Carl Pulfrich.

Some historical notes on Carl Pulfrich.


Histopathology of extracardiac tissues in endomyocardial fibrosis.


Early management of facial trauma.

Early management of facial trauma.