Microbial factors that affect the success of microbial insecticides. Environmental factors external to the host.


Assay and standardization of microbial insecticides.


Insect control programs inplemented by the United Nations.


Regulation of insect populations by microorganisms, Establishing priorities: governmental perspectives.


Systems analysis of insect populations.

Systems analysis of insect populations.


Means for regulation: viruses.

Means for regulation: viruses.


Means for insect regulation: fungi.

Means for insect regulation: fungi.


Definition and identification of insect pathogens.


Urinary complications of lipomyelomeningocele.


Abortive assembly of the lactose transport system in Escherichia coli.


Transport system assembly and the mobility of membrane lipids in Escherichia coli.


Ribosomal ribonucleic acid maturation and synthesis after ribonucleic acid bacteriophage infection.


The interaction of bacterial lipopolysaccharide with phospholipid bilayers and monolayers.


Syphilitic immunofluorescence in experimental relapsing fever.


Cysteines in yeast enolase.

Cysteines in yeast enolase.


Treponemal haemagglutination test.

Treponemal haemagglutination test.


Treatment of gonorrhoea with single oral doses of ampicillin plus probenecid. I. Comparison with procaine penicillin.


Treatment of gonorrhoea with single oral doses of ampicillin plus probenecid. II. Comparison of results in London and Wales.


Treatment of gonorrhoea with amoxycillin.


Single-session treatment of uncomplicated gonorrhoea in men, using penicillin combined with cotrimoxazole. Controlled trial comparing four different treatment schedules with observations on antibiotic sensitivities of gonococci and a review of the literature.


Characterization of a further microimmunofluorescence serotype of Chlamydia: TRIC type G.


Infection of the eye, genitalia, and rectum by a new serotype (type G) of TRIC agent. Clinical and laboratory aspects.


Karl Menninger.

Karl Menninger.


An appreciation of Karl Menninger's books.


Effect of arginine on glucose turnover and plasma free fatty acids in normal dogs.


Separation and radioautography of microgram quantities of ribosomal proteins by two-dimensional polyacrylamide gel electrophoresis.


Immunochemical study of the malabsorption syndrome with cow's milk intolerance. The effect of cow's milk provocation on the immunoglobulins in the intestinal mucosa, intestinal juice, stool extracts, as well as on the levels of antibodies to cow's milk and beta-1C/A in serum, was studied in 18 infants with the malabsorption syndrome. The infants improved on breast milk and were therefore suspected to be intolerant to cow's milk and challenged with it. At the time of clinical relapse a marked and uniform increase in the numbers of IgA- and IgM-containing cells, 2.4 times as many on the average as before challenge, was observed in the jejunal mucosa of eight reacting patients at nine provocations with cow's milk. There was no abnormality in the immunoglobulin-containing cells before challenge or in biopsy specimens taken shortly after the resumption of the elimination diet (breast milk). The clinical reaction was preceded by a pronounced rise in the IgA and IgM contents of stool extracts, and a rise in the titre of haemagglutinating antibodies and serum IgA, changes which subsided rapidly after withdrawal of cow's milk. There was no evidence that the reaction was IgE-mediated or complement-consuming. In some of the 10 patients who tolerated cow's milk challenge clinically there was an increase in both IgA- and IgM-containing cells suggestive of a local immunological reaction although no clinical intolerance was provoked and other immunological signs were weak or absent. In other non-reacting patients no immunological reaction was noted. Therefore it is evident that cow's milk is the agent responsible for the strong local intestinal reaction coinciding with the clinical symptoms.


Art therapy: a diagnostic and therapeutic tool.


Art therapy: a therapeutic technique.

Art therapy: a therapeutic technique.


Discussion of "Art Therapy: a Diagnostic and Therapeutic Tool".


Author's reply to "The Schreber Case, Sixty Years Later".


Increased diagnostic reliability in schizophrenia: some values and limitations.


Current studies on schizophrenia.

Current studies on schizophrenia.


Formation of cyclobutane-type pyrimidine dimers in RNA by sunlight.


Gustav Bychowski, M.D.

Gustav Bychowski, M.D.


Differentiation of Trichoplusia ni MEV and Autographa californica MEV by macrophage migration inhibition tests.


Abraham Colles, 1773-1843.

Abraham Colles, 1773-1843.


Irish blood--and electrolytes.

Irish blood--and electrolytes.


Pitfalls in the diagnosis of acoustic and other cerebellopontine angle tumors.


Septal dermoplasty for von Willebrand's disease in children.


Plastic sheeting in tympanoplasty.

Plastic sheeting in tympanoplasty.


An up-date on franchised dental practices, denturists, and the A.D.A. Relief Fund.


How dentists view Medicaid in Massachusetts.


Topical anesthetic effectiveness. An old and new product evaluated.


Subacute spongioform encephalopathy--a clinical and pathological study with attention to liver involvement.


Polymyositis: new light on pathogenesis and treatment.


Geomedical aspects of neurological cryptococcosis.


Influence of geometry of transplanted tooth and bone on transformation of fibroblasts.


Immunologic responses of mice fed diets supplemented with selenite selenium.


Immunologic studies in patients with chronic active hepatitis and primary biliary cirrhosis. I. Cytotoxic activity and binding of sera to human liver cells grown in tissue culture.


Surgery-induced alterations in plasma recognition factor activity in normal renal donors and renal recipients.


Hector Berlioz (1803-1869).

Hector Berlioz (1803-1869).


In memoriam. Emmet Blackburn Bay 1901-1973.


Vaccinia virus implicated in diffuse demyelinating disease.


In memoriam. Derrick Tilton Vail 1898-1973.


In memoriam. Edward Leland Jenkinson 1892-1972.


Prenatal detection of genetic disorders.


The structure and function of chromatin.


In memoriam. Francis Loeffler Lederer 1898-1973.


Histopathology and distribution of viral antigens in hamsters infected with virulent and benign Venezuelan encephalitis viruses. Lethalities and virulences of Venezuelan encephalitis (VE) viruses for hamsters were found to correlate with severity of histopathologic lesions in hematopoietic and brain tissues. Highly virulent strains (subtype I) destroyed marrow and lymphoid cells rapidly and produced intestinal wall damage; focal brain hemorrhages and destruction of Purkinje cells also occurred within the 4 to 5 days between subcutaneous inoculation and death. Like subtype I virus, a slightly less virulent strain (subtype II) also caused necrosis of bone marrow and brain lesions, but only minimal lymphoid cell damage occurred. The less virulent subtype III VE virus, which killed hamsters between 4 and 14 days after inoculation, usually caused no lesions in hematopoietic tissues, and deaths were related chiefly to hemorrhagic brain lesions and necrosis of Purkinje cells. Two VE viruses, benign for hamsters (the TC-83 attenuated vaccine strain and subtype IV), usually caused no necrosis of hematopoietic or brain tissues; focal extravasations of blood and swollen glial cells were found in brains of the rare hamsters that died. The degrees of necrosis seen in tissues stained with hematoxylin and eosin correlated with the quantities of viral antigens detected by fluorescent antibody, except in pancreas and small intestinal smooth muscle and glands, where antigens of subtype I virus were present without morphologic damage.


The first section: laboratory: American Public Health Association.


Observation of selected dental services under two prepayment mechanisms.


Bacteremia after liver biopsy.

Bacteremia after liver biopsy.


A study of tissue response to the wear particles of artificial joints.


Prognosis in gastric lymphoma.

Prognosis in gastric lymphoma.


Hiatal hernia with esophagitis. A simplified transabdominal surgical treatment.


March sees 330 active members added.

March sees 330 active members added.


Depot cancer chemotherapy through polymer membranes.


Massive thigh injuries with vascular disruption. Role of porcine skin grafting of exposed arterial vein grafts.


Indications for pretransplant nephrectomy.


Low modulus force-dampening materials for knee joint prostheses.


Improvement in cadaveric renal transplant survival.


Radiation reversal of acute rejection in patients with life-threatening infections.


Cation movements and respiratory response in yeast mitochondria treated with high Ca2+ concentrations.


Translation initiation defects in ribosomes from streptomycin dependent strains.


Concentrations of intermediary metabolites in yeast.


Andrew McKie Reid M.C., T.D., 1894-73.

Andrew McKie Reid M.C., T.D., 1894-73.


Philip Jameson Evans, 1907-1973.

Philip Jameson Evans, 1907-1973.


Derick Tilton Vail, 1898-1973.

Derick Tilton Vail, 1898-1973.


Chromosome number 18.

Chromosome number 18.


Reactivity of sera from patients with renal and liver diseases with membrane antigens on monkey kidney cells.


Collaborative clinical trials.

Collaborative clinical trials.


Systemic lupus erythematosus. Studies of the antibodies bound to skin. Systemic lupus erythematosus is characterized by antibodies demonstrable by immunofluorescence on the renal glomeruli and at the basement membrane area of both normal and involved skin. Acid eluates from glomeruli and from normal-appearing skin of three patients with systemic lupus erythematosus contained an antinuclear antibody. This antibody fixes complement and produces a mixed immunofluorescent pattern. Anti-deoxyribonucleic acid or antiextractable nuclear antigen antibodies may be present. This antibody is concentrated on the skin and glomerular basement membrane in proportion to the total serum IgG concentration. In two cases the skin eluate contains, in addition to the antinuclear antibody, a basement membrane antibody that fixes complement, gives a linear immunofluorescent pattern, and appears to be similar (although not identical) to the pemphigoid antibody.


Abnormalities in the distribution of serum immunoglobulin concentrations in juvenile rheumatoid arthritis. Concentrations of serum IgG. IgA, and IgM were determined in 200 patients with juvenile rheumatoid arthritis. The relative frequency distribution of IgG and IgM approached that of a log-normal curve; however, there was marked skewing of the distribution of the serum concentrations of IgA. The prevalence of selective IgA deficiency was 4%. In order to permit further intragroup comparisons, the serum immunoglobulin concentrations were standardized by comparison to a sex-age matched control group. By this process it was found that there was concordance of the serum levels of IgG with IgA, and IgG with IgM. The standardized concentrations of IgA and IgM were less in females than males. The aberration in distribution of serum IgA concentrations found in this study, and the relative inability of females to respond to their disease by increasing specific serum immunoglobulin levels, add further data supporting the concept of immunodeficiency in the pathogenesis of juvenile rheumatoid arthritis.


In vivo assessment of pulmonary vascular integrity in experimental pulmonary edema. During single pass indicator studies across the lungs [(14)C]urea remains in the vascular compartment, but its molecular size and solubility suggest it might escape abnormally permeable vessels. To test the hypothesis that [(14)C]urea might be used to distinguish pulmonary edema due to acutely increased intravascular pressure from that due to vascular damage by alloxan, we studied [(51)Cr]erythrocytes (r), [(125)I]albumin (a), [(14)C]urea (u), and tritiated water as dilution indicators in the pulmonary circulation of anesthetized dogs. In addition, the adequacy of albumin as an intravascular indicator was evaluated. Indicator curves, blood gases, hematocrit, and vascular pressures were determined during a base-line period and repeated 30 and 60 min after treatment in five groups of dogs: (a) saline control. (b) alloxan edema. (c) epinephrine infusion, (d) volume overload, and (e) left atrial (LA) balloon obstruction.Groups b, d, and e developed a similar degree of edema judging by wet/dry lung weights and histology. Groups a and c did not develop edema. In alloxan edema, differences between the mean transit time volume of u and r (V(v-r)) increased over base line at 30 (P < 0.001) and 60 min (P < 0.02); the differences between the mean transit time volume of a and r (V(e-r)) increased slightly at 30 (P < 0.03) and 60 min (P < 0.02); and V(u-r) significantly exceeded V(a-r) at 30 (mean difference = 9 ml, P < 0.02) and 60 min (mean difference = 11, P < 0.04). In none of the other groups did V(u-r) significantly exceed V(a-r). Thus, comparison of V(u-r) with V(a-r) may permit distinction between "high pressure" and "increased permeability" pulmonary edema. Albumin was not a consistently reliable indicator of intravascular volume as compared with composite red cell and albumin curve.


Cellular requirements for the rejection of skin allografts in rats. The role of bone marrow-derived cells in the rejection of skin allografts in rats was investigated. Lewis rats, rendered tolerant of BN antigens and bearing healthy grafts, were thymectomized, irradiated with 900 rad, and injected with varying doses of either normal isologous bone marrow, normal lymph node cells, and/or lymph node cells presensitized to BN antigens. In some experiments rats were also adoptively sensitized to tuberculin. Results showed that, although necessary for the elicitation of tuberculin skin reactions, bone marrow cells are not needed for the rejection of previously tolerated skin allografts. Rats receiving lymph node cells alone rejected their grafts in about 6-7 days. In addition, rats injected with bone marrow alone also rejected their grafts, although significantly later than did lymph node cell recipients, indicating that rat marrow contains a population of cells capable of reacting to transplantation antigens. These cells were found capable of reacting to major transplantation antigens but not minor as they were ineffective in causing the rejection of Ag-B compatible Fischer skin grafts. From experiments utilizing bone marrow from neonatally thymectomized donors and cells treated with an antiserum to rat T cells, these competent cells in the marrow were shown to be thymus derived.


The life span of IgA plasma cells from the mouse intestine. Tritiated thymidine was injected during the neonatal period into conventional CBA mice. The disappearance rate of tritium-labeled IgA plasma cells was followed by a technique combining immunofluorescence with autoradiography which permitted the identification of the immunoglobulin class of plasma cells with labeled nuclei. In this way, it was possible to calculate that the major population of intestinal IgA plasma cells have a half-life of 4.7 days under physiological conditions of stimulation. It was also found that the maximum life span of both intestinal IgA plasma cells and the IgA, IgG, and IgM plasma cells from the spleen is of the order of 7-8 wk.


Maintenance of skin xenografts of widely divergent phylogenetic origin of congenitally athymic (nude) mice. Congenitally athymic (nude) mice accepted for their lifetime intact skin grafts from distantly related mammals (cat, human) and birds (chicken). They also failed to immunologically reject skin grafts from reptiles (lizards) and amphibians (tree frog), although the skin in these grafts underwent varying degrees of disorganization. A definitive role for the thymic defect in this failure to reject xenografts was established by showing that thymus implantation into nude mice enabled them to reject such foreign skin.


Comparison of the virulence for mice of Salmonella typhimurium given by the intraperitoneal and subcutaneous routes. Salmonella typhimurium strain 1566 administered to mice by the intraperitoneal route was considerably more lethal than the same dose administered subcutaneously. This could be measured in terms of mortality, infectivity and mean survival time of those mice that died.


Enrichment procedures for the isolation of Salmonella, Arizona, Edwardsiella and Shigella from faeces. Strontium selenite A broth incubated at 37 degrees C., and strontium chloride B broth incubated at 43 degrees C., have been found an effective combination for the isolation of Salmonella, Arizona and Edwardsiella from human faeces.Direct plating on deoxycholate citrate agar was superior to enrichment methods for the isolation of Shigella species.Strontium selenite A broth was suitable for the isolation of Salmonella and Arizona bacteria at both 37 degrees and 43 degrees C.Strontium chloride B broth incubated at 43 degrees C. was best for the isolation of Salmonella and Edwardsiella.Modified bismuth sulphite agar, although superior to D.C. agar for the growth of Salmonella and Arizona bacteria, was unsuitable for Edwardsiella and Shigella species.A considerable difference was observed in the distribution of Salmonella, Arizona and Edwardsiella serotypes isolated from humans in remote areas, when compared with isolations from more densely populated urban and agricultural centres.Epidemiological and zoogeographical aspects of host-parasite associations between humans, animals and pathogenic Enterobacteriaceae in Western Australia are discussed.


The occurrence of salmonellas and lactose-negative Arizonas in reptiles in The Netherlands, and a comparison of three enrichment methods used in their isolation. A survey was conducted in 1971 in healthy reptiles supplied to the Central Animal Laboratory of Nijmegen for experimental animal research. In order to determine which salmonella serotypes occur, and whether there are several serotypes per animal, several strains of each positive sample were typed.It was found that 160 of 169 samples contained salmonellas or lactose-negative Arizonas or both, and 95 different serotypes were isolated.Of 127 animals examined individually, 67 were carriers of more than one serotype, 42 animals having two types, 21 three types and 4 animals four types.Three enrichment methods were compared. These were tetrathionate broth incubated at 37 degrees C. (T37) and at 43 degrees C. (T43), and selenite broth incubated at 37 degrees C. (SB). All were incubated for 48 hr. before subculture on brilliant-green agar plates. The enrichment methods T37, T43 and SB produced 99, 125 and 123 positive samples respectively, when taken separately. The combinations of T37 and T43, T37 and SB, and T43 and SB produced 145, 142 and 150 positive samples respectively.The yield of serotypes in comparable samples showed no difference between the three enrichment methods. With the use of two methods the yield increased by about 38% compared with one method, and the combination of three methods showed an increase in serotype yield of about 64% compared with one method. A distinct preference by serotypes for definite enrichment methods was not proved.


Preservation of Staphylococcus aureus with unstable antibiotic resistance by drying. Cultures of Staphylococcus aureus showing unstable resistance to kanamycin, streptomycin, lincomycin and penicillin have been preserved by drying. Particular attention has been paid to organisms showing linked resistance to pairs of these antibiotics. Recoveries of viable cells from the desiccates was high and the proportion of resistant and sensitive cells was maintained both during storage and during heating. The preservation of the organisms on ceramic beads stored in air over silica gel promises to be an effective and convenient method for maintaining staphylococcal desiccates during studies of unstable antibiotic resistance.


Evidence for participation of granulocytes in the pathogenesis of the generalized Shwartzman reaction: a review.


Dysentery in general practice: a study of cases and their contacts in Enfield and an epidemiological comparison with salmonellosis. During a laboratory study of diarrhoea in a large urban area during the years 1953-68, 5319 Sonne dysentery infections in 2620 households and six cases of Flexner dysentery in five households were confirmed. The annual incidence per 10,000 population fluctuated between 2 and 31 with an average of 12.Shigella sonnei was found in 10% of new cases of diarrhoea investigated. Plural infections were found in 58% of the households studied and 30% of all contacts examined were shown to be infected. Among these contacts the infection rate was higher for children (42%) than adults (20%), and higher for adult females (24%) than males (16%). The duration of infection was under 2 weeks in nearly half the index cases followed up and longer than 2 months in only 3%. Intermittent excretion was observed in 15%. Repeated Sonne dysentery infection and illness was observed after intervals as short as 5 months. Antibiotic sensitivity was recorded after 1955 and colicine typing after 1960. Changes in antibiotic sensitivity were noted. Children of primary-school age were most susceptible to Sonne dysentery, but symptoms in ill cases were as severe in adults as in children. During years of high incidence cases were concentrated within a small number of primary schools.Sh. sonnei infections were compared with salmonella infections studied concurrently. Sonne dysentery was a winter disease and most cases were under 11 years old, and case-to-case infection was the usual means of spread. Salmonellosis was prevalent in summer and affected nearly as many adults as children, often persons sharing an article of food. It was more prolonged and severe than Sonne dysentery, but less infectious. In all these respects S. typhimurium occupied an intermediate place between Sonne dysentery and ;other salmonellas'.


Treatment and prevention of intravascular coagulation with antiserum to endotoxin.


Contributions of the classical and alternate complement pathways to the biological effects of endotoxin.


Affinity of endotoxin for membranes.

Affinity of endotoxin for membranes.


Patterns of tolerance to endotoxin.

Patterns of tolerance to endotoxin.


Involvement of a chemical moiety of bacterial lipopolysaccharide in production of interferon in animals.


Cellular and molecular aspects of the immune response to a bacterial somatic antigen.


Immunologic responses of mice to lipopolysaccharide from Escherichia coli.


Fate of endotoxin in macrophages: biological and ultrastructural aspects.


Role of O-antigen (lipopolysaccharide) factors in the virulence of Salmonella.


Selective effects of bacterial endotoxins on various subpopulations of lymphoreticular cells.


Molecular organization of the rigid layer and the cell wall of Escherichia coli.


Delineation of the thalamic nuclei with a microelectrode in stereotaxic surgery for parkinsonism and cerebral palsy.


Further information on a "hot spot" in the liver.


Recurrent haematuria and glomerular IgA deposition.


Intradural spinal bronchiogenic cyst. Case report.


The mesangium in membranous glomerulonephritis.


Intrarenal fibrin deposition and its relation to prognosis in proliferative glomerulonephritis.


Alpha 1 antitrypsin deficiency and liver disease in childhood.


Sequential rat-to-mouse skin xenografts.


A fine structure study on hemocyanin formation in the slug Limax sp.


The ultrastructure of epidermis in lichen ruber planus as revealed by electron microscopy. 3. Stratum corneum.


Studies on the intercalated disk of rat left ventricular myocardial cells.


Ultrastructure of nerves and associated cells in bronchiolar epithelium of the mouse lung.


Observations on the fine structure of the nuclear ring of the mammalian spermatid.


The ultrastructure of ferritin macromolecules. I. Ultrahigh voltage electron microscopy (1-3 MeV).


Film supporting frames for mounting serial section grids.


Intracisternal fibers in the liver cells of starved frogs.


Determination of intracardiac shunts in patients with congenital heart disease by means of analog simulation of radiocardiogram.


Clinical studies of myocardial computer scintigraphy with 131-I-MAA injected directly into the coronary artery.


Hypercalcemia after renal transplantation. Long-term follow-up data.


Neonatal Seizures.

Neonatal Seizures.


Parkinson's disease. Clinical analysis of 100 patients.


Relief of pain of Fabry's disease by diphenylhydantoin.


Comparative surface potential patterns in obstructive and nonobstructive cardiomyopathy.


Mechanisms of cardiac arrhythmias: from hypothesis to physiologic fact.


The nitroblue-tetrazolium dye test and infection in the renal patient.


President's page.

President's page.


The sites occupied by some parasitic helminths in the alimentary tract of vertebrates.


Non-shivering thermogenesis and its thermoregulatory significance.


Comparative studies on the isolation of "sublethally injured" salmonellae in nine European laboratories. Artificially or naturally contaminated minced meat samples were distributed to nine laboratories and the isolation rates obtained using standardized pre-enrichment and direct enrichment methods were compared. In all laboratories the pre-enrichment method gave higher isolation rates but the difference was less marked with the naturally contaminated samples. Extending the duration of incubation of the liquid enrichment medium to 48 h and making a second subculture improved the isolation rates. The results obtained with selective plates of the laboratories' own choice were unexpectedly poor in comparison with the standard method.


Feasibility of computer screening of blood films for the detection of malaria parasites. This paper considers the feasibility of automatic screening procedures for detecting malaria parasites in blood smears prepared in the field. A simple programme for pattern recognition by computer has been developed for thin blood films that identifies parasites as densely stained particles within an erythrocyte. The parasites are detected correctly, but platelets falling accidentally within the outline of an erythrocyte and perhaps reticulocytes may be wrongly classified as parasites. The results indicate that automated detection of parasites is technically feasible but that the technique needs to be refined to reduce the incidence of false positives. A substantial increase in processing speed is required if the automatic procedure is to be economically feasible in screening programmes. The complexity of the equipment and the need for well prepared blood smears point to a possible use of the technique in laboratory checks but not under usual field conditions.


A comparative study of daily and twice-weekly continuation regimens of tuberculosis chemotherapy, including a comparison of two durations of sanatorium treatment. 2. Second report: the results from 12 to 24 months. Further results of a controlled clinical trial of tuberculosis chemotherapeutic regimens, carried out with the cooperation of all 7 tuberculosis hospitals and 27 of the 30 chest clinics of the Central Bohemian region and 6 of the 10 chest clinics of Prague, are presented. Following 3 months of standard triple chemotherapy, a comparison was made of 3 continuation regimens allocated at random for up to 18 or 24 months. These were PAS plus isoniazid daily, streptomycin plus isoniazid twice a week, and streptomycin plus isoniazid twice a week up to 1 year and then streptomycin plus isoniazid once a week. After 2 years the results of all 3 regimens were highly satisfactory. Continuation of chemotherapy beyond 18 months produced no further demonstrable benefit at 2 years. A subsidiary comparison between 2 durations of sanatorium treatment-3 months and 6 months-failed to demonstrate any benefit from the longer period. It is concluded that all 3 continuation regimens were equally effective but that the intermittent regimens have the advantage of stricter control over the patient's chemotherapy.


Suture technique for internal mammary-coronary artery bypass without optical magnification.


A cooperative trial on the primary prevention of ischaemic heart disease using clofibrate: design, methods, and progress. The paper describes the design of, and the procedures used in a double blind randomized trial to determine whether the incidence of ischaemic heart disease can be lowered by the reduction of high and moderately high lipid levels in healthy men aged 30-59 years. The trial started in Edinburgh in 1965 and was extended to Prague and Budapest in 1966 and 1967. It is coordinated and controlled by a committee of investigators convened by the World Health Organization. The subjects were selected on the basis of a preliminary determination of serum cholesterol level. Half of the men in the upper third of the distribution of cholesterol values have been assigned at random to a treated group and take 1.6 g of clofibrate daily; the other half make up a control group and take identical capsules containing 300-350 mg of olive oil. A second control group, chosen at random from the lowest third of the cholesterol distribution, also receives the olive oil capsules. The study is designed to have a 90% chance of detecting, in the treated group, a reduction of one-third in the incidence of ischaemic heart disease if this should occur. The subjects are examined at the beginning of the trial, then at 6-month intervals for 2 years, and thereafter annually for at least 3 further years. The criteria of ischaemic heart disease are defined and the different control procedures are described. The required 15 000 subjects have now been admitted to the trial and their characteristics are described, but it is too early to report any results.


Alclofenac (Prinalgin).

Alclofenac (Prinalgin).


Colostomy in the trauma patient.

Colostomy in the trauma patient.


Colonic and rectal complications of kidney transplantation in man.


Our new president: Albert I. Mendeloff.

Our new president: Albert I. Mendeloff.


Collar-button ulcers of the colon in a case of shigellosis.


List of available training programs.

List of available training programs.


Pentazocine: a review of its pharmacological properties, therapeutic efficacy and dependence liability.


Giemsa banding in an established line of human malignant meningioma.


Physico-chemical and reassociation properties of E. coli ribosomal subunits.


A study of neonatal diarrhoea.

A study of neonatal diarrhoea.


Purification of tyrosine: tRNA ligase from Saccharomyces cerevisiae alphaS288C.


A cytogenetic study of recurrent abortion.


An enzyme reducing adenosine 1N-oxide in Escherichia coli, amine N-oxide reductase.


Action of pepsin on synthetic substrates. II. Diglycyl-L-phenylalanyl-L-phenylalanyl (and L-tyrosyl)-glycines: sensitive synthetic substrates for pepsin.


A peptide inhibitor of luteinizing hormone-releasing hormone (LH-RH).


Luteinizing hormone-releasing factor (LRF) in plasma of postmenopausal women.


25-Hydroxycholecalciferol (25-OHD3). II. Efficacy of parenteral administration in prevention of parturient paresis.


Error in estimation of contained milk volume in the dairy goat with cerium-141.


American physicians in the nineteenth century.


Emotional aspects of vasectomy. A review.


Renal-transplant ureteral obstruction simulating retroperitoneal fibrosis.


The struggle to reform the Royal College of Physicians, 1767-1771: a sociological analysis.


George Budd (1808-1882) and nutritional deficiency diseases.


The paradoxical text 'On the Heart'. II.


The history of sutures.

The history of sutures.


Some old books and ancient coins from the Hunter Collection.


Medical education in Edinburgh, 1790-1826, and some Victorian social consequences.


A research note--material of medical interest in the Cromartie papers.


Scottish medical missionaries in Central Africa.


Venereal diseases in sixteenth-century England.


Geriatrics one hundred years ago.

Geriatrics one hundred years ago.


A 'singular calamity'.

A 'singular calamity'.


Nucleotide sequence of a ribosome binding site on RNA synthesized in vitro from coliphage T7.


Helper function in antibody synthesis medicated by soluble factor(s).


Patterns of base modification in tRNA.

Patterns of base modification in tRNA.


Relationship of T cells involved in cell-mediated immunity and antibody synthesis.


Recognition sequence of a restriction enzyme.


Elongation factor T altered in a temperature-sensitive Escherichia coli mutant.


Recurrent hydramnios in association with myotonia dystrophica.


The fate of pyrimidine dimers in ultraviolet-irradiated Chlamydomonas.


Replication of bacteriophage phiX-174 in a mutant of Escherichia coli defective in the dnaE gene. Strains of E. coli bearing a temperature-sensitive mutation at the dnaE locus were unable to support growth of bacteriophage varphiX-174 at 43 degrees while permitting normal growth at 30 degrees . The dnaE gene product was implicated in all stages of varphiX-174 DNA replication. Differences were observed between a double mutant (pol A(-), dnaE(ts)) and a single mutant (pol A(+), dnaE(ts)) in the ability to synthesize parental replicating form and its subsequent replication. The implications of these observations are discussed in terms of the relationship between DNA polymerases-I and -III.


Actin in dividing cells: contractile ring filaments bind heavy meromyosin. Many microfilaments and microtubules are well preserved after glycerol-extraction of HeLa cells at room temperature (22 degrees ). Incubation in heavy meromyosin from rabbit skeletal muscle results in conspicuous and characteristic "decoration" of microfilaments of the contractile ring. Decoration is completely prevented by 10 mM ATP or 2 mM pyrophosphate, and fails to occur if heavy meromyosin is either omitted or replaced by egg albumin, a nonspecific protein. Decorated microfilaments have a substructure consisting of polarized, repeating arrowheads 27-35 nm apart. The specificity of these results strongly suggests that microfilaments of the contractile ring in HeLa cells are closely related to muscle actin. Very thin undecorated strands among the microfilaments of the contractile ring possibly represent a myosin component. These findings are discussed in terms of: the actomyosin-like properties of the contractile ring as a mechanochemical organelle that causes cell cleavage; the probable universal occurrence of actin-like protein in all dividing animal cells; and the contractile ring's combined sensitivity to cytochalasin B and its affinity for heavy meromyosin, a combination unique among microfilamentous organelles.


A new form of DNA polymerase 3 and a copolymerase replicate a long, single-stranded primer-template. A new form of DNA polymerase III, termed Pol III star (Pol III(*)), has been purified to homogeneity from Escherichia coli. Pol III(*) is temperature sensitive when isolated from a thermo-sensitive dnaE mutant, as had been described for Pol III. Pol III(*) and Pol III are separable by gel filtration. Pol III(*) utilizes a duplex template containing short gaps with the same catalytic properties as Pol III. However, Pol III(*) is able to replicate long, singlestranded templates such as homopolymer chains and viral circles of M13 and varphiX174 if provided with the following: spermidine, a primer fragment, and a new protein, termed copolymerase III(*) (Copol III(*)). The latter, purified to homogeneity, has no known independent enzymatic activity and supports synthesis by Pol III(*) but not by Pol I, Pol II, or Pol III.


A DNA-binding protein induced by bacteriophage T7. A DNA-binding protein has been purified from Escherichia coli infected with bacteriophage T7 by DNA-cellulose chromatography. The protein is absent in uninfected cells. The purified protein has a molecular weight of 31,000 and binds strongly and preferentially to single-stranded DNA. In vitro studies show that this protein can stimulate the rate of polymerization catalyzed by the T7-induced DNA polymerase 10-15 times under conditions where the polymerase is unable to effectively use a single-stranded template. The degree of stimulation is dependent upon the ratio of binding protein to DNA template and is independent of polymerase concentration. The observed stimulation is specific for the T7 DNA polymerase in that addition of the protein to reactions catalyzed by E. coli DNA polymerases I, II, or III or T4 DNA polymerase is without effect.


Role of an electrical potential in the coupling of metabolic energy to active transport by membrane vesicles of Escherichia coli. Membrane vesicles from E. coli can oxidize D-lactate and other substrates and couple respiration to the active transport of sugars and amino acids. The present experiments bear on the nature of the link between respiration and transport. Respiring vesicles were found to accumulate dibenzyldimethylammonium ion, a synthetic lipid-soluble cation that serves as an indicator of an electrical potential. The results suggest that oxidation of D-lactate generates a membrane potential, vesicle interior negative, of the order of -100 mV. In vesicles lacking substrate, an electrical potential was created by induction of electrogenic efflux of K(+) with the aid of the K(+) ionophores, valinomycin and monactin. These conditions induced transient accumulation by the vesicles of [(14)C]proline and other metabolites. Experiments with inhibitors and ionophores indicate that neither ATP nor the respiratory chain is involved; the electrical potential generated by K(+) efflux is coupled directly to the transport systems. The results verify two predictions derived from Mitchell's chemiosmotic hypothesis: respiring vesicles generate an electrical potential of the proper polarity and magnitude; and a membrane potential is in itself sufficient to drive the active transport of amino acids and other metabolites.


Latent carcinoma of the prostate.

Latent carcinoma of the prostate.


In memoriam: Dirk du Plessis.

In memoriam: Dirk du Plessis.


The ovarian etiology of human breast cancer.


[History of the control of tuberculosis in Moravia].


How to treat nosebleed.

How to treat nosebleed.


Ferrokinetics in patients with malaria: normoblasts and iron incorporation in vitro.


Freeze fracturing and freeze drying of renal tissue for scanning electron microscopy.


Infective endocarditis in patients treated for malignant neoplastic diseases: a postmortem study.


An approach to microbiologic diagnosis and matrix problems for the small hospital laboratory using a small computer.


Latex-cryptococcal antigen test.

Latex-cryptococcal antigen test.


Further studies on the lytic and neutralizing action of human serum on T. brucei.


Phage typing of Shigella sonnei.

Phage typing of Shigella sonnei.


Repeated immunization of rabbits with sheep red blood cells. II. Influence of frequency of administration of repeated doses of antigen on appearance of PFC-direct and PFC-indirect.


Repeated immunization of rabbits with sheep red blood cells. 3. PFC-direct and PFC-indirect in the blood and spleen after single and repeated immunization.


Lipid analysis of rat enamel and dentine.


The effects of oral inoculation of the yeast and mycelial phases of Candida albicans in rats fed on normal and carbohydrate rich diets.


Three-dimensional features of human enamel as seen with the dissecting microscope.


Blackwater fever in Kenya: problems of etiology.


The pulmonary surfactant.

The pulmonary surfactant.


The aging of alpha-crystallin: a review.


Human cataractogenesis: a review.

Human cataractogenesis: a review.


Prevention of postmyelographic and postpneumoencephalographic headache by single dose intrathecal methyl-prednisolone acetate.


Heparin in migraine headache.

Heparin in migraine headache.


BC-105 and deseril in migraine prophylaxis. (A double-blind study).


Stem cell renewal in spleen and bone marrow of mice after repeated total-body irradiations.


A cybernetic model for radiation reactions in living cells. I. Sparsely-ionizing radiations; stationary cells.


The role of the epidermal cells in moulding the surface pattern of the cuticle in Rhodnius (Hemiptera).


The incorporation of DL-(3-14C)cysteine during spermatogenesis in Ceratopteris thalictroides.


The localization of trypsin in cultured mammalian cells.


Spigelian hernia. Report of a case and summary of literature.


Dual stressed retention sutures.

Dual stressed retention sutures.


The effect of potassium sulphite on the recovery of micro-organisms from dehydrated onion.


External field electromagnetic measurement of blood flow. An alternative approach to the solution of the baseline problem.


Anti-Coa and severe haemolytic disease of the newborn.


The opiates and their derivatives.

The opiates and their derivatives.


Nonbarbiturate sedatives.

Nonbarbiturate sedatives.


Alcohol.

Alcohol.


Cocaine.

Cocaine.


Psychoactive drugs. Amphetamines.

Psychoactive drugs. Amphetamines.


Use of diuretics in nonedematous states.


A case of guttural pouch mycosis caused by Aspergillus nidulans.


Advances in small animal anaesthesia, 1972.


The disease factor in plans for the domestication of wild ruminants in Africa.


Babesiasis on the Island of Arran, Scotland.


Oxalate metabolism. II.

Oxalate metabolism. II.


Bovine congenital defects: arthrogryposis and associated defects in calves.


Carbon monoxide, vitamin B6, and multiple sclerosis: a theory of interrelationship.


Differentiation of salmon poisoning disease and Elokomin fluke fever: fluorescent antibody studies with Rickettsia sennetsu?


Efficacy of bacillus Calmette-Guérin and isoniazid-resistant bacillus Calmette-Guérin with and without isoniazid chemoprophylaxis from day of vaccination. I. Experimental findings in guinea pigs.


Efficacy of bacillus Calmette-Guérin and isoniazid-resistant bacillus Calmette-Guérin with and without isoniazid chemoprophylaxis from day of vaccination. II. Field trial in man.


Superior vena cava syndrome caused by Nocardia mediastinitis.


Training programs in respiratory disease. 1973 (eighth) edition.


Hazards in the operating theatre. Hazards of respiratory circuits.


Hazards in the operating theatre. Contamination of air by anaesthetics.


Oral surgical aids to prosthetic success.


Improved preparation of bacteriophage T4-infected Escherichia coli for the isolation of polynucleotide kinase.


Colostomy. Indications, management and complications.


Polynucleotides associated with histone preparations. Histone fractions prepared from various sources by acid extraction or by salt dissociation contained small quantities of ribo- and deoxyribo-nucleotides. These nucleotides were small and heterogeneous and were efficiently removed from histone preparations by ionexchange chromatography. They probably represent contaminants, rather than uniquely complexed nucleotides, of all the histone preparations.


Interaction between Escherichia coli ribosomal proteins and 5S RNA molecules: recognition of prokaryotic 5S RNAs and rejection of eukaryotic 5S RNAs.


IF3-interference factors: protein factors in Escherichia coli controlling initiation of mRNA translation.


The measurement of total body water in living pigs by deuterium oxide dilution and its relation to body composition.


Computer aided transverse body-section radiography.


Gamma-camera renography using 123I-hippuran.


M. R. C. McWatters.

M. R. C. McWatters.


Fatal endotoxic shock of biliary tract origin complicating transhepatic cholangiography.


Doris McWalter.

Doris McWalter.


R. J. Hunter.

R. J. Hunter.


A. A. McDonald.

A. A. McDonald.


Differentiation of astrocytes in the cerebellar cortex and the pyramidal tracts of the newborn rat. An immunofluorescence study with antibodies to a protein specific to astrocytes.


Serochemotherapy against a Moloney virus-induced leukemia.


Influence of genotype of host on regression of solid and ascitic forms of sarcoma 180 and effect of chemotherapy on the solid form.


Modification of proteins by 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (nsc 79037) in vitro.


Basis for loss of therapeutic effectiveness of L-asparaginase in sensitized mice.


Computer technique for identification of chromatographic peaks.


A critical appreciation of James Strachey's paper on the nature of the therapeutic action of psychoanalysis.


Structure and reconstruction: a critical comparison of Michael Balint and D. W. Winnicott.


The vicissitudes of projection during an analysis encompassing late latency to early adolescence.


Projective identification as a mode of perception and behaviour in families of adolescents.


Toward "archetype" in the Joseph Colombo shooting.


A surgeon reviews a half century of progress in the treatment of coronary heart disease.


Biochemical observations during a trial of inosiplex in senile dementia.


Immunity and asparaginase suppression of tumor growth.


Serum LH levels in bulls treated with synthetic luteinizing hormone-releasing hormone-follicle stimulating hormone-releasing hormone (LH-RH-FSH-RH).


New method for the rapid quantitation of immunofluorescence.


Tumor regression mediated by Mycobacterium bovis (strain BCG). Effects of isonicotinic acid hydrazide, cortisone acetate, and antithymocyte serum.


Experimental toxoplasmic myocarditis in rabbits.


Antibodies in normal and leukemic cats to feline oncornavirus-associated cell membrane antigens.


Kidney preservation for transplantation.


The pathogenesis of xanthomata.

The pathogenesis of xanthomata.


The effects of a synthetic preparation of gonadotrophin releasing factor on pituitary and ovarian function in anoestrous ewes.


Prolonged extracorporeal oxygenation for cardiorespiratory failure after tetralogy correction.


Albert Hyman: 1893-1972.

Albert Hyman: 1893-1972.


Cell and tissue interactions in regenerating muscles.


Multiplicity of muscle changes postulated from motoneuron abnormalities.


Oxygen uptake by striated muscle.

Oxygen uptake by striated muscle.


Mechanisms of growth and atrophy of skeletal muscle.


The present status of intravenous urography. The inaugural Sarel Oosthuizen oration.


An anatomical analysis of the treatment of prolapse of the rectum.


Determining optimum dose-time-area relationships in curative radiotherapy of cancer. A review and evaluation.


Exocytosis-endocytosis coupling in the pancreatic beta cell. The stimulation of the release of insulin by glucose is accompanied by an enhanced uptake of cytochemically demonstrable horseradish peroxidase into endocytotic vesicles within the beta cells. An exocytosis-endocytosis coupling might represent a mechanism by which membrane constituents are recycled within the beta cells under conditions of increased secretory activity.


Male specific antigen: homology in mice and rats. Male lymphoid cells from some strains of rats can sensitize C57BL/6 female mice against C57BL/6 male skin grafts; this indicates that the male specific antigen of these two species is homologous.


Serratia arthritis: report of three cases.


Elimination of surgical wound infections by delayed primary closure.


Psychotropic drug treatment of mixed anxiety and depression in nonpsychiatric office patients: expected and unexpected findings--comparing doxepin, chlordiazepoxide and placebo.


Tuberculous endometritis.

Tuberculous endometritis.


Recent advances in the epidemiology and control of parasitic gastroenteritis in sheep.


In vitro development of first- and second-generation schizonts of Eimeria contorta Haberkorn, 1971 (Coccidia, Sporozoa).


Comparison of inverted centrifugation, saline washing, and dextran sedimentation in the preparation of leukocyte-poor red cells.


Influence of long-term corticosteroid treatment on glucose tolerance in patients with rheumatoid arthritis.


Simple methods for productions of HL-A antigen poor red blood cells.


K.R. Eissler's Discourse on Hamlet. A symposium. Caviare to the General.


K.R. Eissler's Discourse on hamlet. A symposium. Hamlet and ressentiment.


Leukocyte contamination of red cells in leukocyte-poor and frozen-deglycerolized units.


K.R. Eissler's Discourse on Hamlet. A symposium. The victims and the victimizers.


K.R. Eissler's Discourse on Hamlet. A symposium. Reflections on Eissler's doxaletheic function.


K.R. Eissler's Discourse on Hamlet. A symposium. Hamlet as theatre and as a play.


K.R. Eissler's Discourse on Hamlet. A symposium. The journeys in King Lear.


Experimental studies on the mechanisms of closure of cardiac valves with use of roentgen videodensitometry.


Biplane roentgen videometric system for dynamic (60-sec) studies of the shape and size of circulatory structures, particularly the left ventricle.


Videodensitometric system for measurement of vessel blood flow, particularly in the coronary arteries, in man.


Fiedler on acute interstitial myocarditis (1899). I.


Alpha-chain disease in subSaharan Africa.


Pituitary responsiveness to synthetic luteinizing hormone-releasing hormone during pregnancy: effect on follicle-stimulating hormone secretion.


Candida albicans arthritis treated with flucytosine.


Meningitis and cephalothin therapy.

Meningitis and cephalothin therapy.


Invasive enteropathic Escherichia coli dysentery. An outbreak in 28 adults.


Effect of low-level carbon monoxide exposure on onset and duration of angina pectoris. A study in ten patients with ischemic heart disease.


Cholestyramine in type II hyperlipoproteinemia. A double-blind trial.


Epizootic salmonellosis in an open zoo aviary.


Total keratoplasty.

Total keratoplasty.


The lens.

The lens.


Hearing loss in renal disease: clinical and pathological studies.


An analysis of the effects of septal lesions on negatively reinforced behavior.


Tympanoplasty: long-term results.

Tympanoplasty: long-term results.


Cochlear potentials and electron microscopy applied to the study of small cochlear lesions.


The bacterial mesosome.

The bacterial mesosome.


Incus interposition techniques.

Incus interposition techniques.


Coupling between Na+ and sugar transport in small intestine.


In vitro localization of 67Ga in exfoliated epithelial cells from the uterine cervix.


Biochemical, morphological, and immunological observations of leukemic reticuloendotheliosis.


Differential lethal effect of cytotoxic agents on proliferating and nonproliferating lymphoid cells.


Difficulties designing clinical trials as exemplified by a phase 2 drug evaluation of 5(3,3-bis(2-chloroethyl)-1-triazenol)-imidazole-4-carboxamide and 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea in patients with disseminated breast cancer.


Francis Loeffler Lederer, M.D. September 18,1898-April 3, 1973.


Unconsidered trifles no. 23. The Salvation Army comes to Mashonaland.


Circumferential tympano-mastoid access. The sinus tympani area.


Marthinus helperus (Tommie) Steyn.

Marthinus helperus (Tommie) Steyn.


Miraculous healing in the Gospels.

Miraculous healing in the Gospels.


Direct anastomosis of the bilateral internal mammary artery to the distal coronary artery, without a magnifier, for severe diffuse coronary atherosclerosis.


Does cardiac transplantation significantly prolong life and improve its quality?


Local anticoagulation of prosthetic heart valves.


Ontogeny of the immune response in cold-blooded vertebrates.


DNA base composition in mycoplasma, bacteria and yeast.


Variations in the release of LH in response to electrochemical stimulation of preoptic area and of medial basal hypothalamus during the estrous cycle of the rat.


Gas-liquid chromatography in the analysis of mercury (II) compounds.


Toxicity and health threats of phthalate esters: review of the literature.


Organotin compounds: industrial applications and biological investigation.


Malformations of kidney and urinary tract in common chromosomal aberrations. I. Clinical studies.


Anaesthesia for cardiac surgery.

Anaesthesia for cardiac surgery.


Anaesthesia and resuscitation in difficult environments.


Drugs for producing dissociative states.


Recent advances in anaesthesia and analgesia. Complications.


Interaction of drugs associated with anaesthesia.


Obstetric analgesia.

Obstetric analgesia.


Malformations of kidney and urinary tract in common chromosomal aberrations. II. Morphogenetic studies.


Renal homotransplantation. Angiographic features in first 180 days following surgery.


Pneumothorax following lung abscess in the renal transplant patient.


A preliminary report on the clinical use of the microvalve.


A report of dental findings in a survey of geriatric patients.


The circulating swing and the treatment of behavioural abnormalities: views of a pioneer Irish medical psychologist.


Studies of the antibodies in pemphigoid and pemphigus.


Patterns of pulmonary alveolar macrophage function following radiation injury.


Correction of retrognathia by modified "C" osteotomy of the ramus and sagittal osteotomy of the mandibular body.


Correction of maxillary protrusion with severe vertical dysplasia--a multidisciplinary approach.


Cerebral blood flow and brain metabolism as indicators of cerebral death. A review.


A method for improving roentgenographic illustrations for publication--blurred undersubtraction (BUS).


An epidemic of E. Coli type 055:K59(B5) in a neonatal unit.


Alfred Barrett Jameson.

Alfred Barrett Jameson.


Patricia Bassett.

Patricia Bassett.


William Edgar Adams.

William Edgar Adams.


Richard John Gillett.

Richard John Gillett.


Hemostatic "cerclage" after knife conization of the cervix.


Pulmonary function studies during the first year of life in infants recovering from the respiratory distress syndrome.


Intermittent prednisone therapy in children and adolescents after renal transplantation.


Constipation and urinary tract infection.


Employed mothers and their families (I.).


Antibacterial effectiveness of routine hand washing.


Gonococcal ophthalmia neonatorum and routine screening of pregnant women for gonorrheal infection.


Short-term effects of marijuana smoking on cognitive behavior in experienced male users.


Behavioral effects of L-dopa in schizophrenic patients.


A double-blind evaluation of a new hypnotic--flurazepam hydrochloride.


Subdural empyema caused by Salmonella typhimurium.


Malacoplakia of the vagina. First case report.


Frontal encephaloceles.

Frontal encephaloceles.


Anaesthesia for emergency caesarean section: propanidid versus thiopentone.


Edward Baker Trehair.

Edward Baker Trehair.


Ernst Johannes Joubert.

Ernst Johannes Joubert.


Difetarsone in the treatment of Trichuris trichiura.


The nitroblue-tetrazolium test in the investigation of febrile patients.


Drug trials in private practice.

Drug trials in private practice.


Five year follow-up results of operations for duodenal ulcer.


Experience with renal transplantation in high risk patients.


A clinical evaluation of gallium-67 citrate scanning.


Polycystic renal disease treated by renal transplantation.


The management of penetrating wounds of the colon.


Microbial evaluation of reusable and disposable laparotomy sponges.


Carrel's direct transfusion of a five day old infant.


Prevention of wound infections by topical antibiotics in high risk patients.


Selective splenectomy in chronic renal failure.


Treatment of liver abscesses. A conservative surgical approach.


Tests for emotionality in rats and mice: a review.


Agonistic behaviour of juvenile gulls, a neuroethological study.


Edwardsiella tarda infections in Zaïre.

Edwardsiella tarda infections in Zaïre.


Gentics of transfer RNA.

Gentics of transfer RNA.


The effects of endotoxin on cell detachment in vitro.


Biology of the bifidobacteria.

Biology of the bifidobacteria.


The purification and properties of a single chicken pepsinogen fraction and the pepsin derived from it. 1. Evidence is given for the presence of at least five pepsinogens in a crude extract of mixed chicken stomachs. One of these was purified and could be activated to yield a single pepsin. 2. The molecular weights of the pepsinogen and pepsin were 36000 and 34000 respectively. The pepsin associated at low pH values and low ionic strength. 3. The amino acid analyses of both proteins are given. The pepsin was devoid of phosphate but contained carbohydrate. 4. The N-terminal amino acids of pepsinogen and pepsin were serine and threonine respectively. Five amino acids were released by carboxypeptidase A and it was deduced that serine may be the C-terminal one. 5. Each protein contained one thiol group per molecule as determined by titration with p-chloromercuribenzoate. The rate of the reaction was very rapid with pepsin, but much slower with pepsinogen, although the same group appeared to react in both instances. The enzymic activity of pepsin was unaffected by the modification. 6. The isoionic point of the pepsin was close to pH4.0 and the enzyme was stable for long periods at pH values up to 7.0. 7. The enzyme hydrolysed bisphenyl sulphite almost as rapidly as did pig pepsin A.


A procedure for the rapid purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase. A rapid procedure involving DNA-cellulose chromatography followed either by sedimentation in a high-salt glycerol gradient or by gel filtration is described for the complete purification of Escherichia coli DNA-dependent RNA polymerase.


A quick measure of achievement motivation.


Chemistry and biology of the polyene macrolide antibiotics.


The mechanism of action of beta-galactosidase. Effect of aglycone nature and -deuterium substitution on the hydrolysis of aryl galactosides. 1. Steady-state kinetic parameters for the beta-galactosidase-catalysed hydrolysis of 13 aryl beta-d-galactopyranosides show no simple dependence on aglycone acidity. 2. alpha-Deuterium kinetic isotope effects (k(H)/k(D)) for seven of these substrates, measured under steady-state conditions with [S]>>K(m), vary from 1.00 for poor substrates to 1.25 for hydrolysis of the galactosyl-enzyme. 3. Methanolysis of the galactosyl-enzyme in 1.5m-methanol increases K(H)/k(D) for degalactosylation, but leaves that for hydrolysis of ;slow' substrates unchanged. 4. These data are incompatible with a simple two-step mechanism. A scheme consisting of a conformation change, liberation of a galactopyranosyl cation in an intimate ion-pair, non-productive but preferential collapse of the ion-pair to a covalent species and reaction of the galactosyl enzyme through the ion-paired form is proposed. 5. This scheme is used to rationalize previously puzzling data about the enzyme mechanism.


Immunological properties of teichoic acids.


The critical negative.

The critical negative.


Dr. Vernon Douglas Schaffner.

Dr. Vernon Douglas Schaffner.


Thomas Ralph Sarjeant, M.D.

Thomas Ralph Sarjeant, M.D.


Camptothecin.

Camptothecin.


Failure of endogenous gastrin release to affect serum insulin.


Antisera against subcellular lymphocyte fractions in rats and extension to horse antihuman ALS.


Protective effect of cysteine and methylprednisolone in lead acetate-endotoxin induced shock.


Measles encephalitis produced in suckling rats.


What is wrong with a man who is never tired?.


Decubitus ulcers: prevention and treatment.


The behaviour of subcutaneous implanted dermal grafts in guinea pig.


Non-ionic ammonia (NH3) production by bacteria.


Escherichia coli serotypes prevalent in urinary tract infections at Amritsar.


Functional, metabolic and structural changes in the chronic canine cardiac autotransplant.


Reorganization of accident departments.

Reorganization of accident departments.


Sir Reginald Watson-Jones.

Sir Reginald Watson-Jones.


Fracture of the shaft of the femur: a tale of woe.


Successful treatment of Candida septicaemia in severely burned patients with a new antimycotic, clotrimazole (Bay b 5097).


Bone-grafts in the treatment of delayed union of tibial shaft fractures.


A sensitive method for determination of deoxyribonuclease (endonuclease) activity using (lambda-dv) DNA and a nitrocellulose membrane filter.


Studies on the polypeptide elongation factors from E. coli. 3. Molecular characteristics of EF-Tu-guanosine diphosphate, EP-Ts, and EF-Tu-Ts complex.


Isolation of a ribonuclease associated with the formation of phage lambda-cI.


Ribosome degradation and degradation products in starved Escherichia coli. VI. Prolonged culture during glucose starvation.


Antibiotics literature index--a select bibliography.


Studies on kallikrein inhibitors from potatoes. V. Characterization of two isolated inhibitors.


Computer based simulated patient for teaching history-taking.


Effect of pH on the stability of collagen molecule in solution.


Polynucleotides. XVII. Effect of the diphosphates of aristeromycin and cycloadenosine on the polymerization of adenosine diphosphate by polynucleotide phosphorylase.


Pulmonary Nocardia asteroides. Successful treatment with sulfadiazine.


Significance and incidence of Aschoff body in surgically removed auricular appendage. (A review).


Evaluation of the immunofluorescent test in typhoid.


Auditory processing factors in language disorders: the view from Procrustes' bed.


Comparison of orciprenaline, ephedrine and methoxyphenamine as oral bronchodilators.


Harold Edgar Harris.

Harold Edgar Harris.


William Alec Priest.

William Alec Priest.


Morris Netterville Watt.

Morris Netterville Watt.


Alexander Smaill Frater.

Alexander Smaill Frater.


The adrenergic innervation of bronchi in autotransplanted canine lungs.


In memoriam. Graham Lorraine Hilton-Barber.


Research note: some reservations on the use of self-rated class.


The New York Academy of Sciences. New members.


Experiences in the clinical use of polyglycolic acid as an absorbable synthetic suture material.


Inherited lysosomal storage diseases: an essay in comparative medicine.


Clinical evaluation of antiarrhythmic drugs.


Factors affecting the reliability of screening tests for bacteriuria. I. Nitrite test (Urnitest), Uriglox and dip-slide (Inculator).


Factors affecting the reliability of screening tests for bacteriuria. II. Dip-slide: false positive results following postal transport and false negatives owing to incubation at room temperature.


Factors affecting the reliability of screening tests for bacteriuria. 3. Sensitivity and specificity of the Uriglox and various nitrite tests, including a study of the reliability of the Urnitest as assessed by the screened persons.


Dye dilution technique with nephroangiography for the determination of renal blood flow and related parameters.


The diazo method. A simplified method of preparing multicolor combination images for use in roentgen diagnosis.


Cineangiographic estimation of the left ventricular volume during acute myocardial ischaemia in dogs.


Quasi-elastic scattering of laser light. A new tool for the dynamic study of biological macromolecules.


Bacteriological study of Mycobacterium habana.


Characterisation of Mycobacterium, Nocardia, Corynebacterium and related taxa.


A study on Mycobacterium (?) rhodochrous cultures.


Dantrolene sodium in athetoid cerebral palsy.


Urinary incontinence: control by external device.


The effect of substitution at C-2 of D-glucose 6-phosphate on the rate of dehydrogenation by glucose 6-phosphate dehydrogenase (from yeast and from rat liver). 1. The deoxyfluoro-d-glucopyranose 6-phosphates are substrates for both yeast and rat liver glucose 6-phosphate dehydrogenase. 2. The V(max.) values (relative to d-glucose 6-phosphate) were determined for a series of d-glucose 6-phosphate derivatives substituted at C-2. The V(max.) values decreased with increasing electronegativity of the C-2 substituent. This is consistent with a mechanism involving hydride-ion transfer. 3. 2-Deoxy-d-arabino-hexose 6-phosphate (2-deoxy-d-glucose 6-phosphate) showed substrate inhibition with the yeast enzyme but not with the rat liver enzyme. 4. 2-Amino-2-deoxy-d-glucose 6-phosphate (d-glucosamine 6-phosphate) was a substrate for the yeast enzyme but a competitive inhibitor for the rat liver enzyme. 5. Lineweaver-Burk plots for the d-glucose 6-phosphate derivatives with yeast glucose 6-phosphate dehydrogenase were biphasic.


The effect of Nivemycin on pain and swelling following lower third molar removal.


Routine tests for CEA.

Routine tests for CEA.


Early acute rejection of renal homografts: a characteristic clinical syndrome. Seven of 74 patients with early functioning cadaveric renal homografts developed acute oliguric renal failure after the second but before the ninth day post-transplantation. The syndrome characteristically begins with an abrupt and simultaneous decrease in creatinine clearance, urine volume and urine sodium concentration. After a variable period and despite a reduction in immunosuppressive therapy, a diuretic phase ensues and renal function is restored. Complications associated with the syndrome include groin hematoma, pulmonary edema and renal rupture with shock. Renal rupture does not require nephrectomy: if the hemorrhage is controlled, the transplanted organ will resume function. Angiographic studies show normal nephrograms, stretched arterial vasculature and filling defects in the veins. Percutaneous renal biopsy shows interstitial edema and hemorrhage, venous congestion and tubular necrosis. Evidence is presented to support the hypothesis that this is a form of rejection occurring as the result of injury to the renal venous system.


The treatment of chronic osteomyelitis with clindamycin. Clindamycin was given orally over prolonged periods to 12 patients with chronic osteomyelitis. It was well tolerated with only minor side effects and no major toxicity. Clinical and bacteriological cure occurred in five patients and improvement was noted in another five. It appears less effective than either lincomycin or cloxacillin but, because of the complex nature of both the disease and its treatment, strict comparisons are very difficult.


School attainment of seven-year-old children with hearing difficulties.


A genetic study of anencephaly and spina bifida in Glasgow.


Pure-tone acuity, speech-hearing ability and deafness in acoustic trauma. A review of the literature.


Hugo Fricke, 1892--1972.

Hugo Fricke, 1892--1972.


Professor J. J. Weiss, 1905-1972.

Professor J. J. Weiss, 1905-1972.


The synthesis of DNA by membrane-DNA complexes from E. coli B-r and Bs-1 after exposure to fast electrons and neutrons: the measurement of oxygen-enhancement ratios and RBE values.


Roster of members. Kansas State Dental Association.


Current status of bonded attachments.

Current status of bonded attachments.


Federal role in dental care.

Federal role in dental care.


The key to dental care; money or motivation?


McLean's vantage points. Master plan supports team approach.


Extrinsic and central nervous control of colonic motility. A survey of previous and new concepts based on experimental results.


Cryptococcal meningitis in pregnancy.

Cryptococcal meningitis in pregnancy.


Nuclease for DNA apurinic sites may be involved in the maintenance of DNA in normal cells.


Species-characteristic isoenzymes of two aminotransferases in trypanosomes.


Vitamin K and prothrombin synthesis.

Vitamin K and prothrombin synthesis.


Diet and cancer of the colon.

Diet and cancer of the colon.


Dissolution of gallstones.

Dissolution of gallstones.


Overfeeding in the first year of life.

Overfeeding in the first year of life.


Nutrition classics from The Biochemical Journal 29:1273-1285, 1935. CLIV. The antihaemorrhagic vitamin of the chick.


George James and the new nutrition: community medicine as a spearhead of comprehensive health services.


Plasticizers and intravenous therapy.

Plasticizers and intravenous therapy.


Dietary phosphorus, PTH, and bone resorption.


Government and health.

Government and health.


Adrenocortical function in malnutrition.


The role of transferrin in iron absorption.


The role of the medical school in the delivery of health care.


George James, M.D.: bibliography.

George James, M.D.: bibliography.


Morphine dependence in rats with medial forebrain bundle lesions.


Effects of cannabidiol and of diphenylhydantoin on the hippocampus and on learning.


Effect of 6-hydroxydopamine, 5,6-dihydroxytryptamine and raphe lesions on the antinociceptive actions of morphine in rats.


Classic pages in obstetrics and gynecology: Gabrielis Falloppii: Observationes anatomicae ad petrum mannam medicum cremonensem. Venetiis, M. A. Ulmum, 1561.


Solitary malignant lymphoma of the uterus.


Rectal injuries.

Rectal injuries.


Quinine-tetracycline and quinine-bactrim treatment of acute falciparum malaria in Thailand.


The chemotherapy of rodent malaria. XVI. Primary resistance to mepacrine in the N67 strain of Plasmodium berghei-type parasite.


Malaria studies in vitro. I. Techniques for the preparation and culture of leucocyte-free blood-dilution cultures of Plasmodia.


The chemotherapy of rodent malaria. 18. The action of some sulphonamides alone or with folic reductase inhibitors against malaria vectors and parasites. 5. The blood schizontocidal action of some newer sulphonamides.


The chemotherapy of rodent malaria. XVII. Dynamics of drug resistance. 3. Influence of drug combinations on the development of resistance to chloroquine in P. berghei.


Detection of antibodies to Babesia bigemina in dried blood samples using the indirect fluorescent antibody test.


The removal of leucocytes from malaria infected blood.


An electrophysiological study of the central connections of primary afferent nerve fibres from the dental pulp in the cat.


Masticatory movement in the absence of teeth in man.


Application of the camphene technique in the examination of teeth by scanning electron microscopy.


Competitive inhibition and substrate activity of uridine diphosphate 6-deoxygalactose for Escherichia coli uridine diphosphate galactose 4-epimerase. UDP-6-deoxygalactose inhibits the UDP-galactose 4-epimerase (EC 5.1.3.2) from Escherichia coli in a competitive manner with respect to the substrate UDP-galactose, giving K(i) 1.3x10(-3)m. As a substrate for the enzyme, it is transformed into UDP-6-deoxyglucose, although the reaction stops before equilibrium is attained. Possible causes of this behaviour are discussed.


Interaction of quinacrine mustard with mononucleotides and polynucleotides. 1. The interaction between quinacrine mustard and mononucleotides and polynucleotides was investigated by fluorimetry and absorbance spectrophotometry. 2. The fluorescence spectrum of quinacrine mustard is independent of the ionic strength and pH. The dependence of the quinacrine mustard fluorescence intensity on ionic strength, pH and anions is described. 3. The fluorescence intensity of quinacrine mustard was enhanced with the mononucleotide adenylic acid and polynucleotides such as poly(rA), poly(rU) and poly(rA,rU). 4. Quenching of the fluorescence intensity of quinacrine mustard occurred with the mononucleotide guanylic acid and with poly(rG) and poly(rC,rG). 5. The mononucleotide cytidylic acid or poly(rC) showed no effect on the fluorescence intensity of quinacrine mustard. 6. The interaction between the dye and native DNA species was also dependent on the presence of base-specific binding sites in the DNA. The higher the (G+C) content was in the native DNA tested the higher was the quenching effect on the fluorescence intensity of quinacrine mustard. 7. No interaction was found between the dye and methylated DNA. The binding between quinacrine mustard and apurinic DNA was confirmed to be in the phosphate groups of the purines.


Plasmodium berghei: inhibition by splenectomy of a paralyzing syndrome in infected rats.


Evidence for the amino acid sequence of porcine pancreatic elastase. The preparation and purification of tryptic peptides from aminoethylated Dip-elastase and [(14)C]carboxymethylated Dip-elastase, and of peptic peptides from native elastase is described. A summary of the results of chemical studies used to elucidate the amino acid sequence of these peptides is presented. Full details are given in a supplementary paper that has been deposited as Supplementary Publication SUP 50016 at the National Lending Library for Science and Technology, Boston Spa, Yorks. LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1973), 131, 1-20. These results, together with those from previously published papers, are used to establish the complete amino acid sequence of elastase, which is a single polypeptide chain of 240 residues, molecular weight 25900, containing four disulphide bridges.


Respiratory and cardiovascular responses resulting from intravenous injection of sheep hydatid cyst fluid to dogs.


Site of inactivation of melanocyte-stimulating hormone-release-inhibiting hormone by human plasma.


Onchocerca volvulus and Wuchereria bancrofti: fluorescent antibody staining of frozen homologous sections for diagnosis.


Plasmodium berghei and Eperythrozoon coccoides: antibody and immunoglobulin synthesis in germfree and conventional mice simultaneously infected.


Detection of a specific DNA-cytoplasmic membrane complex in Escherichia coli by equilibrium density centrifugation on sucrose gradients.


Sequence homologies among pyridine nucleotide-linked dehydrogenases: possible partial gene duplications in glyceraldehyde-3-phosphate dehydrogenase.


Amino acid sequence homology in alcohol dehydrogenase.


Gonadotrophin release from human foetal pituitary cultures induced by fragments of the luteinizing hormone-releasing hormone.


Chemical typing of human immunoglobulins E and D.


Influence of tertiary structure upon the rate of isotope hydrogen exchange in C(8) H groups of tRNA purine residues.


Recombination in 3-factor crosses of cytoplasmically inherited antibiotic-resistance mitochondrial markers in S. cerevisiae.


Local and generalized Shwartzman reaction induced in rats with 6-sulfanilamidoindazole arthritis.


Surgical complications following renal homotransplantation. Experience with 37 consecutive transplants.


The carbon dioxide laser in clinical surgery. A preliminary report.


Biochemistry, histopathology and treatment of phosphorus burns. An experimental study.


Preliminary observations on the effects of luteinizing hormone releasing factor and oestradiol-17beta on plasma gonadotrophin levels in the anoestrous ewe.


The radioimmunoassay of oxytocin and vasopressin.


Accumulation of nicotine in pancreatic islets and calcitonin-producing cells in mice and chicks demonstrated by micro- and whole-body autoradiography.


Preparation of antisera to luteinizing hormone releasing factor.


The effect of age on some aspects of obese (ob-ob) mouse pancreatic islet morphology and metabolism.


125-Iodine tracers for steroid radioimmunoassay.


Further studies on classification of bovine strains of mycoplasmatales, with proposals for new species, Acholeplasma modicum and Mycoplasma alkalescens.


A mathematical expression for oxygen-induced death in dehydrated bacteria.


The behaviour of Azotobacter chroococcum in oxygen- and phosphate-limited chemostat culture.


Gene-protein relationships of the alpha-keto acid dehydrogenase complexes of Escherichia coli K12: isolation and characterization of lipoamide dehydrogenase mutants.


Studies on the kinetics of the enzyme sequence mediating arginine synthesis in Saccharomyces cerevisiae.


Metabolic and growth inhibition of Mycoplasma gallisepticum by antiserum.


The range of attractant concentrations for bacterial chemotaxis and the threshold and size of response over this range. Weber law and related phenomena. Attractant was added to a suspension of bacteria (the background concentration of attractant) and then these bacteria were exposed to a yet higher concentration of attractant in a capillary. Chemotaxis was measured by determining how many bacteria accumulated in the capillary. The response range for chemotaxis lies between the threshold concentration and the saturating concentration. The breadth of this range is different for attractants detected by different chemoreceptors. Attractants detected by the same chemoreceptor can have their response ranges in widely different places. Over the center of the response range (on a logarithmic scale), bacteria give similar sized responses to similar fractional increases of concentration, i.e. they respond to ratios of attractant concentration, but the response peaks at the center of the range. The size of the response is different for attractants detected by different chemoreceptors. For a detectable response, a smaller increase in attractant concentration is needed for attractants detected by some chemoreceptors than for attractants detected by others. Although the data are inadequate, it appears that the Weber law may be observed over a wide range of concentrations for some attractants but not for others. In the Appendix we aim to explain some of these results in terms of the interaction of an attractant with its chemoreceptor according to the law of mass action.


A comparison of ultraviolet action spectra for vaccinia virus and T2 bacteriophage.


Stanley Smith Stevens.

Stanley Smith Stevens.


The passage of Plasmodium berghei sporozoites through the salivary glands of Anopheles stephensi: an electron microscope study.


Further asexual development of Eimeria magna merozoites in cell cultures.


Serial section analysis of cells in the microfilariae of Dirofilaria immitis containing neurosecretory-like granules.


Living kidney donors.

Living kidney donors.


Physiological responses of the transplanted human kidney.


Secondary pyeloureterostomy in renal transplant patients.


A study of hospital urinary tract infections.


Indanyl carbenicillin in chronic recurrent urinary tract infections.


Dr. Spink at the University of Minnesota.


A short biography of Wesley William Spink.


Current concepts of pyelonephritis.

Current concepts of pyelonephritis.


Clinical approach to infectious diarrheas.


Modern management of severe staphylococcal disease.


Growing problem of salmonellosis in modern society.


Implications of slow viral diseases of domestic animals for human disease.


Oogenesis and fertilization in Dipetalonema viteae (Nematoda: Filarioidea).


The structure and development of the spermatozoon of Dipetalonema viteae (Nematoda: Filarioidea).


Tissue fluid in rabbits infected with Trypanosoma (Trypanozoon) brucei.


The American Public Health Association as a force for change in medical care.


Health services research and health policy: interactions.


Distant transfer of an island flap by microvascular anastomoses. A clinical technique.


Silicone fluid injections fro facial atrophy. A ten-year study.


Viability prediction in pedicle flaps by infrared thermography.


The effect of silastic sheet dressings on the healing of split-skin graft donor sites.


Reconstructions of the nose and upper lip with nasolabial flaps.


An internal nasal mold, made of silicone rubber, for grafts or flaps inside the nose.


A protein involved in the peptidyltransferase activity of Escherichia coli ribosomes. Cores were prepared from 50S ribosomal subunits by incubation with 0.4 M LiCl/Mg(++) (0.4c cores); 0.8c cores and corresponding SP(0.4-0.8) split proteins were obtained from 0.4c cores. In the fragment reaction 0.4c cores were active, but 0.8c cores were not. Activity of the 0.8c cores could be restored by reconstitution with the SP(0.4-0.8) fraction. The split proteins were separated by DEAE-cellulose chromatography and Sephadex gel filtration. The peptidyltransferase activity is correlated with the amount of protein L11 added to the 0.8c core under reconstitution conditions. Whether protein L11 displays the enzymatic activity itself or is part of the enzymatic center is discussed.


Reconstitution of D-lactate-dependent transport in membrane vesicles from a D-lactate dehydrogenase mutant of Escherichia coli. Membrane-bound, flavin-linked D-lactate dehydrogenase in membrane vesicles of E. coli ML 308-225 is solubilized by extraction with guanidine HCl. When membrane vesicles prepared from a D-lactate dehydrogenase mutant are treated with this extract, they regain the capacity to catalyze D-lactate oxidation and D-lactate-dependent transport. Similar effects are obtained with wild-type membrane vesicles in which D-lactate oxidation and D-lactate-dependent transport have been inactivated by 2-hydroxy-3-butynoate. Although treatment of wild-type vesicles with the extract results in an increased capacity to catalyze D-lactate oxidation, no effect on transport is observed. Reconstituted transport activity is a saturable function of the amount of guanidine extract added. Moreover, the quantity of extract required to achieve maximum initial rates of transport varies with each transport system. On the other hand, reconstituted D-lactate oxidation increases linearly over a broader range of extract concentrations.Oxamate, a competitive inhibitor of D-lactate dehydrogenase, and p-chloromercuribenzenesulfonate block both the initial rate of transport and the steady-state level of accumulation in reconstituted vesicles. Furthermore, these reagents induce efflux of transport substrates from preloaded, reconstituted vesicles. The same reagents inhibit the initial rate of uptake but not the steady-state level of accumulation in ML 308-225 vesicles, and do not induce efflux. These results suggest that, although reconstituted vesicles catalyze D-lactate oxidation and D-lactate-dependent transport, the system has not been reconstituted to its native state.


Effect of thiostrepton on recycling of Escherichia coli initiation factor 2. The recycling of initiation factor 2, which requires both GTP and the 50S subunit and involves GTP hydrolysis, appears to be blocked by thiostrepton. However, this antibiotic has little or no effect on the AUG-directed ribosomal binding promoted by initiation factor 2 of fMet-tRNA under conditions that do not permit optimal recycling of the factor. Evidence is presented suggesting that the 50S ribosomal proteins L7 or L12, which are required for the function of the chain elongation factors Tu and G and the accompanying hydrolysis of GTP, are also involved in recycling of initiation factor 2. Although both 5'-guanylylmethylene diphosphonate and thiostrepton seem to block recycling of initiation factor 2, fMet-tRNA bound to the ribosomes in the presence of GTP and thiostrepton can react with puromycin, whereas that bound in the presence of 5'-guanylylmethylene diphosphonate and the antibiotic cannot. It is proposed that initiation factor 2 may interact with two nonidentical ribosomal sites during polypeptide chain initiation.


Effect of nalidixic acid on DNA replication by toluene-treated Escherichia coli. Nalidixic acid inhibits DNA synthesis in toluene-treated E. coli, strain B/r, as it is well known to do in vivo. Both semiconservative and repair syntheses are affected, though to different degrees. Density-transfer experiments indicate that chromosomal replication is reinitiated when nalidixic acid is removed from toluene-treated cells after exposure to the acid for one generation in vivo. For cells in vivo or after toluene-treatment, reinitiation is not seen in asynchronous cultures exposed briefly to nalidixic acid or in cells prevented from synthesizing proteins during their exposure to the acid. Reinitiation occurs at the chromosomal origin but, unlike the effect seen in vivo, replication at the old site persists.


Effects of selective extraction of histones on template activities of chromatin by use of exogenous DNA and RNA polymerases. Exogenous DNA and RNA polymerases were used to measure the template activity of DNA in chromatin in isolated thymus nuclei from which lysinerich or arginine-rich histones were selectively extracted. Measurements were on nuclei containing 20-1200 mug of DNA per ml, the distinctions becoming clear at the higher concentrations. Experiments with RNA polymerase showed only moderate increases in template activity upon extraction of histone, although the removal of lysinerich histone had a greater effect than that of arginine-rich histone. DNA polymerase action on nuclei minus lysinerich histone achieved high results, exceeding even those on DNA itself.


Cell envelope and shape of Escherichia coli K12. Rod-shaped "ghosts" that are free of murein have been isolated from E. coli. The shape of these "ghosts" is maintained by a unit membrane soluble in sodium dodecyl sulfate. Ghosts consist of about 20-30% phospholipid (almost exclusively phosphatidylethanolamine) and 50-60% protein; a large fraction of the remaining material is lipopolysaccharide. Sodium dodecyl sulfate-gel electrophoresis reveals 4-5 different bands corresponding to molecular weights between 10,000 and 40,000. Treatment of ghosts with Pronase reduces this number to 3, and the rod shape still is not lost. Results of treatment of ghosts with a crude extract from Dictyostelium discoideum have supplied tentative evidence that at least one of these proteins is involved in the maintenance of rod shape. It does not appear too unlikely that these polypeptide chains are the final products of the genetic information specifying cellular shape.


In vitro repression of transcription of the tryptophan operon by trp repressor. The in vitro repression of transcription of the tryptophan operon by the trp repressor from Escherichia coli was studied. By measuring the inhibitory effect for trp-specific RNA synthesis in an in vitro transcription system directed by DNA of trp-transducing phage, we have detected and concentrated a trp repressor in an eluate of a Phi80 ptED native DNA-cellulose column. The repression of transcription of trp operon required the addition of L-tryptophan in the system, and when several tryptophan analogues were added, the repression or derepression was similar to that observed in vivo. The repressor fraction was separated from the majority of tryptophanyl-tRNA synthetase activity by Bio-gel P60 column chromatography.


Determination of secondary and tertiary structural features of transfer RNA molecules in solution by nuclear magnetic resonance. High-resolution 300-MHz proton nuclear magnetic resonance spectra of the hydrogen-bounded protons in three different purified tRNA molecules are presented. The resonances in the region between -11 and -15 ppm from 2,2-dimethyl-2-silapentane-5-sulfonate (DSS) are assigned to the ring NH protons of specific base pairs by two approaches. First, intrinsic positions of -14.8 ppm and -13.7 ppm are taken for the AU and GC ring NH protons, respectively, and the spectra are calculated by including ring current shifts from the nearest neighbors. The spectra calculated in this way on the basis of the cloverleaf are in good agreement with the observed. Second, fragments of yeast tRNA(Phe) were obtained, which helped in assignments of the spectrum of intact molecules. The close agreement strongly supports the cloverleaf model. Tertiary structural features were determined in a few cases where the ring currents at the terminal base pairs of helical regions depended upon stacking of the helices. In this way, we were able to show that in Escherichia coli tRNA(Glu) the CCA stem forms a continuous helix with the TPsiC stem, which is in accord with the preliminary x-ray structure of yeast tRNA(Phe), suggesting that this stacking is observed in solution and may be a general property of different tRNA molecules. Similar reasoning suggests that in E. coli tRNA(fMet) G-27 is stacked upon the dihydrouridine helix.


A genetic method for determining the order of events in a biological pathway. We describe the application of a simple genetic test that indicates the order of events in a morphogenic or biochemical pathway. The test uses temperature-sensitive (ts) and cold-sensitive (cs) mutants, and it orders the times at which the ts and cs defects are expressed.


Mutants of Escherichia coli thermosensitive for the synthesis of transfer RNA. Using a simple three-step procedure, we have isolated thermosensitive mutants of E. coli that are specifically defective in transfer RNA (tRNA) synthesis. Our procedure was designed to identify mutants that are unable to make su(3) (+) tRNA(Tyr) or grow at the restrictive temperature, yet under the same conditions they retain the ability to make mRNA and protein. The mutants obtained have been analyzed, and they are defective in different steps in the synthesis of functional tRNA at the restrictive temperature. Some of them may fail to modify certain bases in the tRNA. Two mutants are unable to process the 5' end of tRNA precursor molecules. Three are unable to cleave precursor molecules at the 3' end. 11 Mutants can not synthesize any tRNA molecules or tRNA precursors. We speculate that these latter mutants may be defective in RNA polymerase or in an RNA polymerase factor specific for stable RNA synthesis.


Relationship of the structure of bacterial lipopolysaccharides to its function in mitogenesis and adjuvanticity. The ability of bacterial lipopolysaccharides to induce lymphocyte mitogenesis and to act as an adjuvant of antibody formation was attributable to the lipid-A region of the molecule. Measured by induction of DNA synthesis, lipid A was mitogenic for bone marrow-derived lymphocytes obtained from spleens of congenitally athymic mice but not for thymocytes obtained from thymuses of normal mice. Adjuvanticity was demonstrated by the ability of lipid A to convert a tolerogenic regimen of antigen into one eliciting an immune response and by its ability to markedly enhance the antibody response to a weak antigen.


Studies on the inducible inhibitor of radiation-induced DNA degradation of Escherichia coli.


The rapair of DNA single-strand breaks in E. coli K-12 x-irradiated in the presence or absence of oxygen; the influence of repair on cell survival.


Nonribosomal synthesis of guanosine 5',3'-polyphosphates by the ribosomal wash of stringent Escherichia coli. A factor in the ribosomal wash of stringent strains of E. coli was identified by Haseltine et al. as a complement to the ribosomal system for the synthesis of the magic spot compounds of Cashel and Gallant. This factor has been found, in the absence of ribosomes, to catalyze the enzymatic pyrophosphoryl transfer from ATP to GTP or GTP in the formation of magic spot I and magic spot II, the guanosine tetra- and pentaphosphates (ppGpp and pppGpp), respectively. The enzyme, which normally requires the presence of the ribosome-tRNA-mRNA complex for activity, catalyzes a very slow synthesis that is stimulated tenfold by 20% methanol. The temperature optimum of the methanol-stimulated system is 25-30 degrees and activity is drastically depressed at 37 degrees , presumably by inactivation. Catalysis is linear with enzyme concentration and with time for the first 3 hr; during this period 25% of the added GTP is converted. The nonribosomal system is distinguished from the ribosomal system by having a lower Mg(++) and a higher NH(4) (+) optimum. The two systems differ in their response to antibiotics: thiostrepton strongly inhibits the ribosomal system but has no effect on the nonribosomal system.


Modification of DNA repair and survival of x-irradiated pol, rec, and exr mutants of Escherichia coli K-12 by 2,4-dinitrophenol.


Bernard Smaller (1918-1972).

Bernard Smaller (1918-1972).


Treatment of volume resistant experimental shock by hypervolemia and hypothermia.


Interaction between the splenic and hepatic arteries in portal hypertension.


A survival analysis of patients undergoing dialysis or renal transplantation.


Sexual adjustment to maintenance hemodialysis and renal transplantation: national survey by questionnaire: preliminary report.


Hyperlipidemia following renal transplant.


Chronic home peritoneal dialysis in children.


Hemodialysis in the management of massive burns.


Water as a biomaterial.

Water as a biomaterial.


Hemodialysis on end-stage lupus nephritis.


American Society for Artificial Internal Organs. Membership list-1973.


Recent results in total artificial heart.


A-V fistulas and the diabetic: ischemia and gangrene may result in amputation.


Organ transplantation: forward.

Organ transplantation: forward.


Landmarks in organ transplantation--a historical review by a kidney transplant recipient.


Ethics of renal transplantation.

Ethics of renal transplantation.


The basis for ethical decisions on clinical transplantation as viewed by a transplant recipient.


The costs of kidney transplantation and hemodialysis.


The Federal involvement in the care of patients with end-stage renal disease: the relationship to the nation's health properties.


Patterns of grief: end-stage renal failure and kidney transplantation.


The thoughts and feelings of a kidney transplant donor.


Preoperative workshops with patients waiting for kidney transplants.


Why transplantation? The kidney twins.

Why transplantation? The kidney twins.


"The agony and the ecstasy".

"The agony and the ecstasy".


Experiences of a renal donor.

Experiences of a renal donor.


Motivations of a living-related kidney donor.


The world is a beautiful place.

The world is a beautiful place.


The problem of cadaver kidney transplantation in children today, as seen by a parent and kidney donor.


A transplant short story.

A transplant short story.


Experiences of a physician-patient after renal transplantation.


Reflections of a transplant recipient after admission to medical college.


Kidney transplant: a personal case history.


Life looks a lot better when you get a second look at it.


Survival with a living-related donor transplant.


Notes from a recipient of two parental kidney transplants.


Life experiences of a cadaver kidney transplant recipient.


Letter to a kidney.

Letter to a kidney.


Life before and after transplantation.

Life before and after transplantation.


Why I have not had a kidney transplant after nine and one-half years as a hemodialysis patient.


The choice between dialysis and transplantation.


The motivation of living donors.

The motivation of living donors.


Damaged self-esteem and depression in organ transplantation.


The transplant nurse.

The transplant nurse.


Current risks to the kidney transplant donor.


The first implantation of an artificial heart: reflections and observations.


Intravenous methylprednisolone sodium succinate: adverse reactions reported in association with immunosuppresive therapy.


Dr. David M. Hume.

Dr. David M. Hume.


Dedication: Dr. D.M. Hume.

Dedication: Dr. D.M. Hume.


A rapidly progressing mesangio-capillary glomerulonephritis in Finnish Landrace lambs.


Aspergillus fumigatus infection in the nasal cavity of a dog: its treatment with amphotericin B.


The clinical use of polyglycolic acid as an absorbable synthetic suture material.


Clostridium oedematiens infection in cattle in Trinidad, West Indies.


Comparative aspects of bacterial lipids.


Physiology of the bdellovibrios.

Physiology of the bdellovibrios.


Drug therapy review--coumarin and indandione derivatives.


Factors influencing the increase in glomerular filtration rate in the remaining kidney of transplant donors.


Relapsing polychondritis with insulin resistance and antibodies to cartilage.


The etiology of subacute infective endocarditis by E. Libman and H. L. Celler. The Americal Journal of the Medical Sciences, vol. 140, 1910.


Clinical and immunologic studies in identical twins discordant for systemic lupus erythematosus.


The use of diazepam in the treatment of tetanus.


Coding properties of isoaccepting lysine transfer RNA species from baker's yeast.


Resistance to microbial infection--vaccines in theory and practice.


The kinetics of induction of  -galactoside permease.


Increased translation in liver involves a supernatant factor following laser irradiation.


Synthesis and properties of a new fluorescent polynucleotide, poly(1,N6-ethenoadenylic acid).


Conformations of N6-monosubstituted adenine derivatives. Crystal structure of N6-methyladenine.


Precipitation of nucleic acids with polyethyleneimine and the chromatography of nucleic acids and proteins on immobilised polyethyleneimine.


Differentiation of 23-S ribosomal RNA cistrons from 16-S ribosomal RNA cistrons in Escherichia coli by the sequence complementarity test.


A dose-response evaluation of androgens in the treatment of metastatic breast cancer.


Cellular responses to autologous breast cancer tissue. Sequential observations.


Validity of thermodilution method for measurement of cardiac output in pulmonary oedema.


The history and development of Harrington instrumentation.


Nosologic and genetic aspects of scoliosis.


The problems of the composition and structure of the mineral components of the hard tissues.


Non-treated scoliosis.

Non-treated scoliosis.


The classic. De rachitide sive marbo puerili, qui vulgo the rickets dicitur: Francis Glisson, M.D.


Uptake of tritiated glycine into neurons of the human retina.


On the stability of N-ethylmaleimide alkylated sulfhydrylgroups.


H-Y antigens on mouse spermatozoa.

H-Y antigens on mouse spermatozoa.


The induction of ovarian dysfunctions in thermobia domestica by the Cecropia Juvenile hormones.


New anti-malarial agent with potential repository effect.


Health care delivery: a public-private consortium.


Patient care evaluation. In the light of public scrutiny.


Interview. Herman M. Somers: 1973 winner of the Justin Ford Kimball Award of the American Hospital Association.


Autohaemolysin-producing cells in mice injected with bacterial vaccines.


The structural basis of cell-mediated immunological reactions of collagen. The species specificity of the cutaneous delayed hypersensitivity reaction.


Localization of intracellular immunoglobulin A in porcine intestinal mucosa using enzyme-labelled antibody. An ultrastructural study.


Recent advances in diagnostic immunology.


Immunohistochemical study of IgA transepithelial transfer into digestive tract secretions in the mouse.


Evaluation of positive end-expiratory pressure in hypoxemic dogs.


Positive-pressure breathing as a protective technique during +Gz acceleration.


Gluconic acid-producing bacteria from honey bees and ripening honey.


Heart rate and blood pressure responses to electrical stimulation of the central nervous system in the pigeon (Columba livia).


The vitamin nutrition of Zymomonas anaerobia.


Numerical taxonomy and laboratory identification of Bacterionema matruchotii, Rothia dentocariosa, Actinomyces naeslundii, Actinomyces viscosus, and some related bacteria.


Isolation of T-mycoplasmas from goats, and the production of subclinical mastitis in goats by the intramammary inoculation of human T-mycoplasmas.


Sulphonamide-induced 'thymineless death' in Escherichia coli.


Growth of Aspergillus nidulans in a thin liquid layer.


Inhibition by 3-deoxy-3-fluoro-D-glucose of the utilization of lactose and other carbon sources by Escherichia coli.