Transfer of aminonucleoside nephrosis by renal transplantation. The pathogenesis of aminonucleoside of puromycin (PA) nephrotic syndrome in rats was studied using renal transplantation to separate systemic from renal factors. The nephrotic syndrome was transferred by transplantation of kidneys from rats with established proteinuria. Bilaterally nephrectomized normal rats receiving kidneys removed as early as 15 min after intravenous PA injection (100 mg/kg) of donors also developed proteinuria (602+/-125 mg/24 hr) and a nephrotic syndrome after the usual induction period of 4-7 days observed in this disease. Arterial perfusion of isolated kidneys with PA (50 mg/kg) followed by perfusion with isotonic saline 3 min later and then transplantation to normal bilaterally nephrectomized rats led to a nephrotic syndrome. Urine protein excretion was 494+/-42 mg on the 7th day after transplantation. In contrast, urine protein excretion after transplantation of normal kidneys to normal bilaterally nephrectomized rats was 40+/-20 mg on the 7th day. Exposure of a normal kidney to a nephrotic host environment by transplantation of a normal kidney to a unilaterally nephrectomized PA-injected rat did not transfer the disease to the normal kidney. After removal of the nephrotic kidney 11-13 days after transplantation, proteinuria of the donor kidney was normal (21+/-13 mg on day 15). These studies indicate that pathogenesis of aminonucleoside nephrosis involves programming of the kidney directly by PA within minutes after exposure although increased urinary protein excretion does not occur until several days later.


Splenic replenishment of synergistic ability to bone marrow and thymic cells of neonatally splenectomized CBA mice. Bone marrow (B) and thymic (T) cells taken from adult mice that had been splenectomized within 24 hr of birth showed an inability to cooperate in the IgM response to sheep red blood cells. The defect in collaborative capacity was apparent in both sets of cells, but appeared to be more pronounced in the T cell population. Splenectomy performed at various neonatal intervals indicated that if removal of the spleen were delayed until 6 days after birth, B and T cells of the adult showed a 60% restoration in cooperation. Replenishment of the synergistic ability after neonatal splenectomy could be achieved by injecting spleen cells immediately after spleen removal or 2 months postsplenectomy.


Surface antigens of immunocompetent cells. 3. In vitro studies of the role of B and T cells in immunological memory. The effect of preincubation with anti-theta or anti-mouse immunoglobulin (Ig) and complement (C') on immune responsiveness of spleen cells from BALB/c mice immunized with sheep erythrocytes (SE) was investigated. Both treatments greatly depressed the remaining ability to produce a secondary response to SE in vitro. Normal BALB/c spleen cells were far less effective in reconstituting the responses of such depleted cell populations than were much smaller numbers of untreated immune spleen cells. Thymus-derived cell (T cell) memory appeared early after immunization and showed specificity for the immunizing antigens. Recombination of anti-Ig-treated with anti-theta-treated immune spleen cells resulted in virtually complete reconstitution of responsiveness. The presence of immunological memory in T cells and the nature of their surface receptors are discussed.


Cellular receptors: binding of radioactively labeled anti-alloantiserum. RAT ALLOANTISERA OF THE FOLLOWING SPECIFICITIES WERE OBTAINED AFTER SINGLE SKIN GRAFT REJECTION: DA anti-BN, BN anti-DA, DA anti-Lewis, and Lewis anti-DA. Anti-alloantibodies were raised by injecting the first two alloantisera into (DA x BN)F(1) hosts and the last two alloantisera into (DA x Lewis)F(1) hosts. In this manner, four anti-alloantisera were raised: anti-(DA anti-BN), anti-(BN anti-DA), anti-(DA anti-Lewis), and anti-(Lewis anti-DA). From each anti-alloantiserum a gamma globulin fraction was prepared and trace labeled with (125)I. The labeled anti-alloantibodies could be shown to be fixed preferentially by red cells coated with the alloantibody used to induce them. They were also preferentially fixed by normal rat lymphoid cells presumed to carry recognition structures similar to alloantibody. For instance, anti-(DA anti-BN) was fixed by BN red cells coated with DA anti-BN and by normal DA lymphoid cells presumed to carry RS(BN). Conversely, anti-(BN anti-DA) was fixed by DA red cells coated with BN anti-DA and by normal BN lymphoid cells, carrying RS(DA). Absorption and inhibition experiments showed that alloantibodies and normal lymphoid cells compete for the same labeled anti-alloantibodies. We conclude that cellular receptors and circulating alloantibody are antigenically similar: They have the same aliotype.


Regulation of lymphocyte responses in vitro. I. Regulatory effect of macrophages and thymus-dependent (T) cells on the response of thymus-independent (B) lymphocytes to endotoxin. DNA synthesis of normal rat spleen cells in response to endotoxin increases markedly if adherent cells are first removed from the cell suspension. Addition of small numbers of purified macrophages to the cultures restores the response to a low level. T-deprived cells show these effects in very much lesser degree. Large numbers of macrophages completely suppress the response of both normal and T-deprived spleen. We conclude that two mechanisms of suppression are at work: a direct effect of macrophages and a macrophage-dependent "suppressor T cell" effect.


Assessment of hepatic respiratory excursion.


PR Forum. Advertising Council.

PR Forum. Advertising Council.


Effects of radiation therapy on bone lesions as measured by  47 Ca and  85 Sr local kinetics.


Heparin-induced iso-antibody responses in myocardial infarction. An illustration of the value of the electronic data processing of clinical records.


Julius Dreschfeld.

Julius Dreschfeld.


Renal transplantation in South Australia.


Surgeon John Harris's grave.

Surgeon John Harris's grave.


The circulatory abnormalities of heat stroke.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 41-1972.


Surgical management of abortion.

Surgical management of abortion.


Mechanisms of habituation in the brain stem.


RBE of pulsed radiations by lethality in mice: cultured mammalian cells and bacteria.


Factors affecting gas exchange by collateral ventilation in the dog.


The 'f-factor' of the spleen-colony assay for hemopoietic stem cells.


Overlap error in counts of splenic colonies.


The metabolism of N-(3-chlorobenzenesulphonyl) acetamidine in the dog and rat.


The metabolism of ( 14 C-methyl)dichlorvos in the rat and the mouse.


Bunolol metabolism by cell-free preparations of human liver: biosynthesis of dihydrobunolol.


Hemodynamic investigation of congenital heart disease in infancy and childhood.


A sensitive immunofluorescence technique for detecting blood group substances A and B. Findings in oral epithelium.


Ocular manifestations in aminoacidopathies.


[The blood circulation of the retina and the uvea].


Mechanical malfunction and thrombosis of prosthetic heart valves.


History of coronary care units.

History of coronary care units.


Wilburt Cornell Davison, MD: a Festschrift.


Caerulein action on gastric secretion in normal and hypoxic rats.


Adult celiac disease, reticulosis and carcinoma.


The Davison Medallion.

The Davison Medallion.


Wilburt Cornell Davison: he built a medical school of stone--and left it marble.


Wilburt C. Davison and medical libraries.


Building a school.

Building a school.


HL-A matching and survival data of 107 cadaveric renal allotransplants.


Fever in the child with myelomeningocele and ileal diversion.


The "inoperable" breast carcinoma. A successful result using zinc chloride fixative.


Epidemic gram-negative septicemia in surgical patients.


Retrograde distal pancreatectomy.

Retrograde distal pancreatectomy.


Transitions in infant sensorimotor development and the prediction of childhood IQ.


Merle M. Miller, M.D., 1903-1972.

Merle M. Miller, M.D., 1903-1972.


Electron microscopy of late interstitial keratitis.


Correlation of type specific fluorescent antibodies to ragweed with symptomatology: double-blind study.


Tear gas spray injury. An unusual case.

Tear gas spray injury. An unusual case.


Mercury pollution by tissue fixatives.

Mercury pollution by tissue fixatives.


Immunopathological studies of Plasmodium berghei-infected mice. Immune complex nephritis.


The gastrectomy operation revisited.

The gastrectomy operation revisited.


Immunofluorescent studies of the arterial lesions in rat cardiac allografts.


Direct measurement of total metabolism of a burned patient.


Krypton 85 washout curves in isolated perfused kidneys.


Renal transplantation without oliguric acute tubular necrosis.


Prepyloric mucosal diaphragm (antral web): report of a case and review of the literature.


Max M. Strumia.

Max M. Strumia.


Isolation, fractionation, and RNA polymerase activity in a sample enriched with oligodendroglia nuclei.


Some ascending connections of the pulvinar and nucleus lateralis posterior of the thalamus in the cat.


Campbell B. Keenan, D.S.O., M.D., C.M., F.R.C.S.(C), 1867 to 1953.


Cholestyramine treatment of ileal exclusion diarrhea.


Origin and organization of the CMA.

Origin and organization of the CMA.


Comparison of medroxyprogesterone and corticosteroids in the treatment of canine renal allografts.


The first decade (VII).

The first decade (VII).


Dr. Peter Banks: profile of a future President accustomed to the hot seat.


Production of renomedullary prostaglandins by renomedullary interstitial cells grown in tissue culture.


Trypanosoma cruzi: interaction with mouse peritoneal macrophages.


Concepts of nematode-fungus associations in plant disease complexes: a review.


Carl Claus as Freud's professor of the new Darwinian biology.


Freud and the Viennese.

Freud and the Viennese.


Chemical and enzymatic studies of deoxyribonucleic acid covalently linked to Ficoll.


Phosphoenolpyruvate carboxylase of Escherichia coli. Purification and some properties.


Measurement of free electrophoretic mobility and retardation coefficient of protein-sodium dodecyl sulfate complexes by gel electrophoresis. A method to validate molecular weight estimates.


On the mechanism of the pentose phosphate epimerases.


Physical properties and subunit structure of xanthosine 5'-phosphate aminase.


Anthranilate synthetase from Serratia marcescens. On the mechanism of tryptophan inhibition.


Presteady state kinetics of phosphorothioate hydrolysis by alkaline phosphatase. Rate-limiting dephosphorylation at alkaline pH.


Denaturation and renaturation of xanthosine 5'-phosphate aminase.


The subclavian steal syndrome. Cause of symptoms in the arm.


Digital transposition in the injuried hand.


B. Aravind Adyanthaya, 1936-1971.

B. Aravind Adyanthaya, 1936-1971.


The pathogenesis of Shigella diarrhea. I. Enterotoxin production by Shigella dysenteriae I. A strain of Shigella dysenteriae 1, freshly isolated from a patient with dysentery in Guatemala in August 1969, was found to elaborate an enterotoxin into the liquid of broth cultures. Partial purification of the enterotoxin by ultrafiltration on graded polymeric membranes and Sephadex gel filtration (Pharmacia Fine Chemicals, Inc., Piscataway, N. J.) suggested an approximate molecular weight of 55,000-60,000. The partially purified toxin was heat-labile, pronase sensitive, and activated by alkaline pH, and it elicited fluid production in ligated rabbit ileal segments; it failed, however, to cause increased vascular permeability in skin. When the activities of equal weights of identically prepared Vibrio cholerae and S. dysenteriae enterotoxins were compared in the rabbit ileum the latter caused a significantly smaller volume response with increased concentrations of potassium, chloride, and protein. The previously described neurotoxic (mouse lethal) factor was also present and eluted from Sephadex G-150 with the enterotoxin. If these biological activities prove to be possessed by a single molecular species, it is suggested that it be renamed Shigella enterotoxin in recognition of the physiologically more relevant biological action.


Permanent implantation of arterial and venous catheters and lymphatic shunts.


Radioimmunoassay of plasma hormones: review of plasma insulin in ruminants.


Identification of bacteriological unknowns: a computer-based teaching program.


Foreign language requirements for graduate students in anatomy.


Survival of the private practice concept of the face of prepaid health care.


Plasma concentration of levodopa in Parkinsonism before and after inhibition of peripheral decarboxylase.


Casimir Funk--a biographical sketch (1884-1967).


The ultrastructural cytology of the nucleolus of Eimeria magna gamonts.


Serology of the Cercarienhüllen Reaktion of Schistosoma mansoni.


Serology of Schistosoma spindale (Montgomery, 1906).


Hymenolepis microstoma cysticercoid activation and excystation in vitro (Cestoda).


Phonetics and swallowing to determine palatal contours of dentures.


The cingulum clasp.

The cingulum clasp.


Influence of a composite resin restoration on the gingiva.


Clinical criteria for posts and cores.

Clinical criteria for posts and cores.


An alternative method of unilateral subperiosteal implant fixation: a preliminary report.


Troubleshooting casting technical problems for dental alloys to which porcelain will be fused.


A new technique for constructing a one-piece hollow obturator after partial maxillectomy.


Recovery from aphasia as monitored by AER audiometry.


The effect of endotoxin on the alveolocapillary permeability coefficient in the dog.


Percivall Pott's disease of the spine, discussed in two letters of J. Hunczovsky from London, 1779, to G. A. Brambilla in Vienna.


Treatment of common parasitic infections of man encountered in the United States. I.


The therapeutic value of children's literature.


Bronchopulmonary dysplasia associated with oxygen therapy in infants with respiratory distress syndrome.


Frederick Henry Lutze, M.D. 1900-1971.

Frederick Henry Lutze, M.D. 1900-1971.


Enzymatic repair of radiation-induced strand breakage in cellular DNA and its chemical inhibition.


133Xe studies of collateral ventilation and air trapping following endobronchial occlusion.


Obstructive urologic malformations of the fetus and infant--relation to neonatal pneumomediastinum and pneumothorax (air-block).


Surgical closure of ventricular septal defect in infancy.


Octosporea carloschagasi n.sp., a microsporidian associate of Trypanosoma cruzi in Panstrongylus megistus.


Escherichia coli O antigen typing by means of a mechanized microtechnique. A mechanized microtechnique for the serological typing of Escherichia coli cultures is described.


Activation of a toxic component of Clostridium botulinum types C and D by trypsin. When the Stockholm and 468C strains of type C and the 1873 strain of type D Clostridium botulinum are "cured" of their prophages, they simultaneously discontinue the production of their dominant toxins (C(1) and D), but they continue to produce a second antigenically monospecific toxin (C(2)). These "cured" strains of types C and D therefore become indistinguishable with respect to the toxin produced. Fifteen type C cultures received from other laboratories discontinued to produce the dominant toxin when subcultured in broth. The C(2) toxin, however, was produced by eight of these cultures. The C(2) toxin is produced by these cultures as a protoxin that requires treatment with trypsin before its toxicity can be demonstrated. Of the 21 type C cultures that produce the C(1) toxin, 20 were shown to produce the C(2) toxin. The filtrates of 14 of these cultures required trypsin treatment before the C(2) toxicity could be demonstrated. Low levels of toxicity could be demonstrated in the six remaining culture fluids without trypsin; toxicity, however, was increased with trypsin.


Gas-volume measurement system for evaluating effectiveness of antimicrobial compounds. Yeast spoilage was followed by measuring gas volume produced inside a sealed bag of inoculated fruit. Volume of gas produced correlates with plate counts.


Tetrazolium agar overlay in test for Mycoplasma pneumoniae. In this rapid presumptive test for Mycoplasma pneumoniae, subculture is not required, and tetrazolium reduction is evident in 1 hr or less.


Use of gelatinase in the routine sampling of gelatin for Salmonella contamination. Gelatinase added to gelatin enrichment broth suspensions decreased viscosity. Salmonellae were not harmed by enzyme. Salmonella isolation procedures can be simplified by eliminating the viscosity problem.


Simplified fluorescent-antibody staining method for primary plate isolates of group A streptococci. Beta-hemolytic streptococci were identified from primary plates by using a simplified fluorescent-antibody staining method. There was 100% correlation between this method and standard precipitin grouping.


Improved hemagglutination test for identifying type A strains of Pasteurella multocida. A simple, improved, indirect hemagglutination test is described for the recognition of Type A strains of Pasteurella multocida. It involves the treatment of mucoid cultures with testicular hyaluronidase. Hydrolysis of the capsular hyaluronic acid presumably releases the specific antigen for adsorption to erythrocytes.


Improved procedure for identification of group D enterococci with two new media. With the use of Pfizer selective enterococcus medium as a screening process, tyrosine decarboxylase (TD) medium and D-broth were devised for additional confirmation of the identification of group D enterococci. The semisolid TD medium was used in a method similar to inoculating a motility tube. TD activity was indicated by clarification of the medium near the line of inoculation because the insolubility of tyrosine provided an otherwise milky suspension. D-broth was used to show the tolerance of organisms to 6.5% NaCl at pH 8.2. In addition, enterococcal species (except Streptococcus durans) could also be demonstrated by the acid formation due to the fermentation of mannitol or arabinose. With this improved system, about 97% of enterococci could be accurately identified and confirmed.


Evaluation of the qualitative and automated quantitative microhemagglutination assay for antibodies to Treponema pallidum. Qualitative and quantitative microhemagglutination assays for antibodies to Treponema pallidum (MHA-TP) were performed on 314 syphilitic and 597 presumably nonsyphilitic sera, and the results were compared with those of the fluorescent treponemal antibody-absorbed (FTA-ABS), the Treponema pallidum immobilization (TPI), and the Veneral Disease Research Laboratory (VDRL) tests. MHA-TP sensitivity was similar to that of the other tests in all stages of syphilis except primary syphilis, in which MHA-TP reactivity was only 64% compared with 82% in the FTA-ABS test, 73% in the VDRL test, and 67% in the TPI test. MHA-TP specificity was satisfactory and comparable to that of the other treponemal tests. Quantitation of the MHA-TP test was automated by use of Autotiter II equipment. Titers tended to become elevated later in the course of syphilis and to remain elevated longer than did VDRL titers. Reproducibility of the quantitative MHA-TP test was satisfactory, with duplicate tests agreeing within one doubling dilution on 97.5% of 351 reactive sera. Poor reproducibility was obtained with sera giving minimal reactions in the qualitative test, and such sera should be routinely retested. The MHA-TP is less time-consuming and costly than the FTA-ABS test and could be used in conjunction with the VDRL or another reagin test for syphilis to eliminate a large number of the FTA-ABS tests now required.


Technique and apparatus for rapid and inexpensive enumeration of bacteria. A foot-operated diluter/dispenser and a projection viewer were developed for use with a rapid bacterial counting technique employing agar droplets. The equipment allows the advantages of the technique to be properly realized and assists many conventional bacteriological tests which may be made at the same time. Significant cost and labor savings are made, with reductions in incubation time, incubator space, and preparative work.


Catalase test as an aid to the identification of Enterobacteriaceae. Although the catalase test has been used for many years for rapid differentiation of the genera of gram-positive organisms, little has been said about its use in the family Enterobacteriaceae. It was further noted that a wide variety of methods exist for the execution of the catalase test, that there is no universally accepted strength specified for the hydrogen peroxide, and that no gradations for the vigor and speed of the reaction have been mentioned. Under the conditions of the clinical laboratory, we have developed a simple, rapid, and accurate method for the catalase test that has been of great value as an aid in the identification of the Enterobacteriaceae. With 3% H(2)O(2), it was observed that Serratia, Proteus, and Providencia were vigorous catalase reactors. Only Salmonella and rare Escherichia, Enterobacter, and Klebsiella isolates were moderate catalase reactors. Escherichia and Shigella strains were mostly nonreactive, with less than one-third weekly (+) reactive, whereas most Enterobacter strains tended to be weakly reactive. Klebsiella strains were divided equally between nonreactive and weakly reactive. In practice, this test was also of great value in discerning nonpigmented Serratia cultured from the hospital environment and in detecting mixed flora containing nonspreading Proteus.


Machine for automatic bacteriological pour plate preparation. A fully automatic system for preparing poured plates for bacteriological analyses has been constructed and tested. The machine can make decimal dilutions of bacterial suspensions, dispense measured amounts into petri dishes, add molten agar, mix the dish contents, and label the dishes with sample and dilution numbers at the rate of 2,000 dishes per 8-hr day. In addition, the machine can be programmed to select different media so that plates for different types of bacteriological analysis may be made automatically from the same sample. The machine uses only the components of the media and sterile polystyrene petri dishes; requirements for all other materials, such as sterile pipettes and capped bottles of diluents and agar, are eliminated.


Early medical conditions in Arizona.

Early medical conditions in Arizona.


Therapeutics XIX. Lipid lowering agents.


Freud's influence on social casework.

Freud's influence on social casework.


The Greek concept of diabetes.

The Greek concept of diabetes.


Sudden whitening of the hair.

Sudden whitening of the hair.


Medical numismatic notes. VII. Mithridates IV.


Excerpts from "An Undelivered and Unorthodox Address".


The medieval crossbow as surgical instrument: an illustrated case history.


Evidence for a lytic enzyme produced by Bdellovibrio bacteriovorus 6-5-S.


The effects of elevated temperatures on spore swelling and germination in Aspergillus niger.


Oxygen requirements of strains of Pseudomonas and Achromobacter.


Aggregates of Azotobacter vinelandii cysts.


Transduction of Salmonella typhimurium genetic material by coliphage P1 in S. typhimurium Hfr x escherichia coli hybrids.


Archives of the Forensic Sciences. Medicolegal contributions of historical interest. The Siamese twins at the College of Physicians. A phonographic report in the Philadelphia Medical Times of Thrusday, February 19, 1874, (Chaing and Eng).


An evaluation of procedures for enumerating bacteria in activated sludge.


Activity of specific and non-specific cholinesterases in the subcommissural organ of guinea pig and albino rat. Light and electron microscopic observations.


Acyl carrier protein. XVII. Purification and properties of  -hydroxyacyl acyl carrier protein dehydrase.


Acetyl coenzyme A carboxylase. IV. Biotinyl prosthetic group-independent malonyl coenzyme A decarboxylation and carbosyl transfer: generalization to other biotin enzymes.


Acyl carrier protein. XVI. Intermediate reactions of unsaturated fatty acid synthesis in Escherichia coli and studies of fab B mutants.


Glutamyl transfer ribonucleic acid synthetase of Escherichia coli. 3. Influence of the 46K protein on the affinity of the 56K glutamyl transfer ribonucleic acid synthetase for its substrates.


Purification, substrate specificity and binding,  -decarboxylase activity, and other properties of Escherichia coli 2-keto-4-hydroxyglutarate aldolase.


Viral modification of the valyl transfer ribonucleic acid synthetase of Escherichia coli.


Purification and properties of a specific Escherichia coli ribonuclease which cleaves a tyrosine transfer ribonucleic acid presursor.


The primary structure of the phase-1 flagellar protein of Salmonella typhimurium. I. The tryptic peptides.


Obsolescence: the physician or the diagnostician role.


The golden jubile of insulin.

The golden jubile of insulin.


New experimental vectors of Colombian Trypanosoma rangeli.


Immune response against hamster erythrocytes in the low-responder mouse strains. 3. Antibody production in the reversed combination of low-responder C57BL-6 mice as donors of antigens and golden hamsters as hosts.


Effect of the live and heat-killed cells of Staphylococcus aureus on the resistance of mice to the infection with Nocardia asteroides.


Formation of multiple drug resistance factors by recombination between transfer factor and resistance determinants.


Studies on  M and  G antibody response of mice to bovine serum albumin. I. A systematic survey of immunizing conditions.


Comparative investigations on the chemical induction of point mutations and dominant lethal mutations in mice.


Induction and repair of gene conversion in UV-sensitive mutants of yeast.


Toxigenic Escherichia coli: a cause of infantile diarrhea in Chicago.


Lethal and mutagenic effects of elevated temperature on haploid yeast. II. Recovery from thermolesions.


Acute respiratory failure in the adult. 3.


Cyanate as an inhibitor of red-cell sickling.


Charles C. Lund, M.D.

Charles C. Lund, M.D.


The randomized clinical trial.

The randomized clinical trial.


Controlled clinical trials denounced.

Controlled clinical trials denounced.


Comments on the adverse effect of concurrent pyridoxine administration on the efficacy of L-dopa in treating parkinsonism.


Surgical treatment of familial plantar hyperkeratosis.


Some biological aspects of cyclopentane chemistry.


Immunosuppressive properties of heterologous antilymphocytic sera prepared against thymocyte soluble fraction. 1.


Defects in the immune response of NZC mice. 1.


In vitro commitment by hemopoietins of murine marrow exocolony forming units (CFU)? Preliminary report. 1.


Immuno-electron microscopy of hepatitis B antigen in liver. 1.


Sjogren's syndrome: its clinical manifestations and associations.


5-fluorocytosine treatment of candida infections of the urinary tract and other sites.


Paraneoplastic erythrocytosis and inappropriate erythropoietin production. A review.


Renal blood flow in man studied with a dye-dilution method.


Hemoglobin interactions and red cell metabolism.


A view of graduate surgical education from the Veterans Administration.


Exploratory laparotomy for staging of lymphomas.


The Geneticists' Manifesto.

The Geneticists' Manifesto.


The complement system in renal homograft recipents.


Structural features in the smooth muscle of the gut and their physiological significance.


The orientation of connective tissue fibres in rat skin.


Ocular manifestations of systemic diseases.


Opportunities for the alert.

Opportunities for the alert.


Georg von Beksey.

Georg von Beksey.


Immune response of the lizard, Tiliqua rugosa.


A rapid assay procedure for ATP:L-methionine adenosyltransferase.


The dentition in a group of mediaeval Scottish children.


Further scanning electron microscope studies of the preparation of class II cavities.


Intestinal intolerance to sugars in children. A review.


Reflection of manic-depressive psychoses in dynamic drawing tests.


An insolubilised pepsin.

An insolubilised pepsin.


Molar absorptivity and A-1 per cent-1cm values for proteins at selected wavelengths of the ultraviolet and visible region. VI.


The Flexners of Louisville.

The Flexners of Louisville.


A model for the demonstration of suturing technics.


Immune response against hamster erythrocytes in the low-responder mouse strains. II. Effects of pre-sensitization with hamster lymphoma cells on antibody production against hamster erythrocytes.


Morphological changes in Staphylococcus aureus and Escherichia coli exposed to cephalexin.


Thomas J. Kinsella, M.D.

Thomas J. Kinsella, M.D.


Early transfer of genes determining transfer functions by some Hfr strains in Escherichia coli K12.


Regulatory mutants of the deo regulon in Salmonella typhimurium.


Regulation of the formation of isoleucyl-tRNA-synthetase and the level of isoleucine biosynthetic enzymes in E. coli K12.


Expression of an episomal lactose gene after conjugational transfer into a thermosensitive DNA mutant of Escherichia coli.


The denturist--a bleak expediency.

The denturist--a bleak expediency.


The morphology of the prepared root canal: a study utilizing injectable silicone.


An evaluation of cardiorespiratory effects of posture in the dental outpatient.


C. Edmund Kells.

C. Edmund Kells.


Regional electroanalgesia and its potentialities in control of orofacial pain.


Health behavior change: a political model.


Position statement on diet and coronary heart disease. American health Foundation.


Laudation Prof. Dr. B.G. Ziedses des Plantes.


Subtraction in cervical pneumomyelography and an application of RISA myelography.


Binocular differentiation of radiographs (the blinking method). A method of detecting changes in the roentgen image retakes.


Effectivity of a new local analgesic Hoe 40 045.


Change of the system for the adsorption of vitamin B 12  to intestinal mucosa homogenate in growing rats.


The role of the juxtaglomerular apparatus.


Simultaneous demonstration of catecholamines and acetylcholinesterase in peripheral autonomic nerves.


Emil Froeschels.

Emil Froeschels.


Donald M. Markle (1923-1972).

Donald M. Markle (1923-1972).


The effects of halothane on the interactions between myocardial contractility, aortic impedance, and left ventricular performance. I. Theoretical considerations and results.


Continuous positive pressure ventilation and oxygen delivery.


The effect of postoperative artificial ventilation on arterial blood oxygenation.


Fat embolism associated with drug overdosage. Case report.


Anaesthetic management of a very premature infant. Case report.


Obituary. Hermenegildo Arruga, 1886-1972.


Femoral aneurysms.

Femoral aneurysms.


The histopathology of small vessels following microvascular repair.


Ventilation in an artificially produced gas cavity under basal conditions.


Cataracts after renal transplantation.

Cataracts after renal transplantation.


Herpes simplex and temporal lobe epilepsy.


Anesthesia by acupuncture.

Anesthesia by acupuncture.


The incidence of Candida albicans in the plaques of teeth of children.


The evolution of the fixed appliance in orthodontic practice.


Stimulatory action of ovine LH releasing factor on synthesis of luteinizing hormone (LH) demonstrated by the rat intratesticular test system.


Volume changes of the pancreatic islets during perinatal days in the rat.


Liver preservation without perfusion.

Liver preservation without perfusion.


Variation of the lipid content of yeast cells during sporulation.


Immunopathological studies on liver biopsies.


The influence of amino acid starvation on the UV reversibility of the strains of Escherichia coli B-r thy - trp - Hcr +  and Escherichia coli B-r thy - trp - Hcr - .


Role of sugars in phosphate transport in baker's yeast.


Ribosomes after T4-infection.

Ribosomes after T4-infection.


The use of exocellular nuclease-negative mutants for the study of the plasmid DNA content of Serratia marcescens.


Effect of chlorpromazine on cell-free protein synthesizing systems of rat brain, rat liver and E. coli.


A cytological study of the ovary of Rhodnius prolixus. Cytoarchitecture and development of the trophic chamber.


Electron microscopic investigations of Klebsiella rhinoscleromatis in culture.


Renal dysplasia in infants with neurospinal dysraphism.


A study on Iraqi date extract: "Dibis" as substrate for mycological fat production.


Pathogenesis for mice of Coxsackie A virus, type 16.


Comparative analyses of strain L-929 and its substrain L-P3 grown in a protein- and lipid-free synthetic medium.


Heterozygotes of three-factor crosses of bacteriophage T4.


An analysis of replication errors made by a defective T4 DNA polymerase.


Mechanism of transcription in the N operon of bacteriophage lambda.


A unique form of terminal redundancy in adenovirus DNA molecules. A unique form of terminal redundancy has been observed in DNA molecules extracted from several human adenovirus serotypes. Electron microscopic studies reveal that single-stranded circular molecules are formed when native DNA is denatured and then annealed. Temperatures approaching the T(m) of native DNA are required to convert circles to linear molecules, indicating a high degree of self-complementarity between terminal base sequences of DNA strands. Single-stranded circles are not generated if a limited number of nucleotides (2-4%) are removed from the 3' ends of native DNA by digestion with Escherichia coli exonuclease III before denaturation and annealing. The lenght of the redundant segment appears to differ among major serotypic groups, and a possible association between increased length of the redundant segment and increased oncogenic capability of virus serotype is suggested. Evidence for the configuration of the duplex closure region of circular molecules is also presented.


Polypeptide chain initiation and stepwise elongation with Artemia ribosomes and factors. The supernatant initiation factor from Artemia salina embryos promotes, besides the AUG-dependent binding of fMet-tRNA(f), the poly(U)-dependent binding of N-acetylPhe-tRNA to 40S ribosomal subunits; the bound N-acylaminoacyl-tRNA reacts directly with puromycin upon addition of 60S subunits. Both the binding reaction and the synthesis of N-acylaminoacyl-puromycin occur in the absence of GTP or other ribonucleoside triphosphates. To a smaller extent, the factor also mediates the 40S ribosomal binding of Met-tRNA(f) and Phe-tRNA; in this case, the bound aminoacyl-tRNA is less reactive with puromycin. After the poly(U)- and supernatant factor-dependent binding of N-acetylPhe-tRNA to 40S subunits at low Mg(2+) concentration, binding of a second aminoacyl-tRNA (Phe-tRNA), with ensuing formation of the first peptide bond, is dependent upon the addition of the 60S subunit, elongation factor EF-1, and GTP. Further growth of the polypeptide chain requires translocation and is, therefore, dependent upon the addition of elongation factor EF-2. As with the Escherichia coli system, once requirements for translation of the third codon have been met, no further additions are necessary for elongation of a peptide chain.


Antigen cap formation in cultured fibroblasts: a reflection of membrane fluidity and of cell motility. Reaction of antibody to surface Histocompatibility-2 antigens of cultured mouse fibroblasts causes aggregation of the cellular antigens into caps; these appear as areas of high antigen concentration localized away from the cell processes. Cap formation is inhibited when ATP generation by cells is interrupted, when the temperature is lowered, by addition of cycloheximide-especially over long time periods-and by colcemid. The observations are consistent with a role of the locomotor system of cells in the collection of small antigen-antibody aggregates into a large localized cap. Together with observations on the absence of caps in epithelial cells, these findings suggest that only cells, such as fibroblasts and lymphocytes, that bear leading ruffled membranes are capable of driving antigenantibody aggregates to form caps.


The gradient-sensing mechanism in bacterial chemotaxis. A "temporal gradient apparatus" has been developed that allows the motility of bacteria to be studied after they have been subjected to a sudden change from one uniform concentration of attractant to another. A sudden decrease elicits the tumbling response observed with spatial gradients; it was found, however, that a sudden increase also elicits a response, namely supercoordinated swimming. This demonstrates that chemotaxis is achieved by modulation of the incidence of tumbling both above and below its steady-state value. The initial responses gradually revert to the steady-state motility pattern characteristic of a uniform distribution of attractant. The apparent detection of a spatial gradient by the bacteria therefore involves an actual detection of a temporal gradient experienced as a result of movement through space. Potential models for the chemotactic response based on some "memory" mechanism are discussed.


DNA-directed synthesis in vitro of T4 phage-specific enzymes. The synthesis of deoxynucleotide kinase (EC 2.7.4.2) in vitro by a preparation consisting of T4 bacteriophage DNA and a cell-free extract of Escherichia coli has been reported. A study of the role of monovalent cations in the synthesis of this enzyme as well as alpha-glucosyl transferase (EC 2.4.1.2) shows that potassium ions are required for maximal enzyme production. Examination of the RNA-directed system indicates that potassium ions are more effective than ammonium ion in the translation of messenger RNA for the formation of the biologically active proteins studied. From a comparison of the magnitude of the effect of ions on both the DNA- and RNA-directed systems, we conclude that potassium ions may also have a marked stimulatory effect on transcription of the deoxynucleotide kinase gene of T4 DNA. The time required for messenger initiation and completion and the size distribution of messenger RNA formed in vitro were also examined.


Isolation of supercoiled colicinogenic factor E 1  DNA sensitive to ribonuclease and alkali. The synthesis of the covalently-closed, circular DNA form of colicinogenic factor E(1) (ColE(1)) continues in Escherichia coli cells after the addition of chloramphenicol. A large portion of the purified supercoiled ColE(1) DNA molecules made in the presence of chloramphenicol are converted to the open circular DNA form after treatment with alkali (pH 13), RNase A, or RNase H. These treatments do not significantly affect the covalently-closed form of ColE(1) DNA isolated from normally growing E. coli cells. The open circular product resulting from treatment of supercoiled ColE(1) DNA with RNase A possesses a single break in one strand of the circular duplex. The site sensitive to RNase A occurs with equal probability in either of the complementary strands. Both synthesis of ColE(1) DNA and the formation of supercoiled ColE(1) DNA sensitive to RNase A or alkali are prevented by the inhibitor of RNA synthesis, rifampicin. These results indicate that covalently-closed ColE(1) DNA containing one or more ribonucleotides accumulates during ColE(1) replication in the presence of chloramphenicol. It is proposed that this incorporated RNA served as a primer during the initiation of synthesis of ColE(1) DNA and that its removal from the circular DNA is inhibited in cells incubated in the presence of chloramphenicol.


Replication of bacteriophage M13: specificity of the Escherichia coli dnaB function for replication of double-stranded M13 DNA. Infection of the temperature-sensitive E. coli mutant HfrH 165/70 (dnaB) with the filamentous single-stranded DNA phage M13 is abortive at the restrictive temperature. Upon infection at 41 degrees , single-stranded phage DNA penetrates the cell and is converted in a rifampicin-sensitive step to the double-stranded replicative form (RF). The parental RF attaches to the cell membrane, but subsequent replication of the RF is blocked. It is concluded that in M13 infection semiconservative RF replication of a double strand to a double strand, in contrast to single-stranded DNA synthesis, depends specifically on the dnaB function.


Lac operator analogues: bromodeoxyuridine substitution in the lac operator affects the rate of dissociation of the lac repressor. As measured by a decreased rate of dissociation, lac repressor binds 10-times tighter to 5-bromodeoxyuridine-substituted lac operator than it does to normal lac operator. This result is obtained both in the absence and in the presence of isopropylthiogalactoside, an inducing ligand. These data are significant with regard to the mechanism of sequence-specific protein-DNA interaction, and also suggest a possible explanation for the effects of bromodeoxyuridine on the expression of differentiated functions in eukaryotic cells.


Correlation between rates of degradation of bacterial proteins in vivo and their sensitivity to proteases. Experiments were undertaken to test whether the relative rates of degradation of proteins in vivo might correlate with their sensitivity to proteases. Various experimental conditions that promote degradation of proteins in Escherichia coli increased sensitivity of average cell proteins to trypsin or other endoproteases, including incorporation of several aminoacid analogs into the proteins, incorporation of puromycin into the polypeptide chain, or frequent errors in translation. Those abnormal proteins that were degraded most rapidly within the cell appear responsible for the increased protease sensitivity of the cell extract. Normal E. coli proteins that rapidly turn over in growing cells are, on the average, more sensitive to trypsin or Pronase than cell proteins that turn over more slowly. The inherent sensitivity of a protein to proteolytic digestion is thus a major determinant of protein half-lives in vivo.


Localization of antibody-combining sites within the variable portions of heavy and light chains. The Fab'-fragment of a mouse IgA-myeloma (protein-315) was split by pepsin to yield a smaller fragment that retained the anti-2,4-dinitrophenyl activity of the intact protein. This fragment, which we call Fv, has a molecular weight of about 30,000 (half that of Fab'), and is composed of two polypeptide chains (molecular weight 14,000) held together by noncovalent bonds. The N-terminal sequence of Fv suggests that it is composed of the N-terminal half of Fab', and consists of the variable portions of the heavy and light chains. Since Fv has about one binding site with the same association constant as Fab', this experiment provides direct evidence that the antibody site in this protein is contained entirely in the variable portion, and is independent of the constant portion, of the molecule.


Relationship of Flac replication and chromosome replication. The time of replication of a bacterial plasmid, Flac, during the division cycle of Escherichia coli has been estimated in exponentially growing cultures and at various times after a shift from minimal medium to a richer medium (a shift-up). There is a variation in the cell age at which the capacity to synthesize beta-galactosidase (beta-D-galactoside galactohydrolase, EC 3.2.1.23) doubles (assumed to be a measure of the time at which the Flac plasmid replicates) when this capacity is measured at various times during the shift-up, and with increasing steady-state exponential growth rate. Cells growing at slow and moderate growth rates exhibit Flac replication in the middle of the division cycle. With increasing time after a shift-up or with increasing growth rate the plasmid replicates at earlier times, eventually at cell division, and finally in the older cells. This variation in the cell age at which the plasmid replicates is similar to the variation in cell age at which chromosome initiation occurs during a shift-up, although plasmid replication occurs slightly before initiation of chromosome replication.


Regions of single-stranded DNA in the growing points of replicating bacteriophage T7 chromosomes. In partially replicated T7 chromosomes, the points where parental strands are separating and new DNA is being synthesized can be seen in the electron microscope to contain regions of single-stranded template DNA. The single-stranded regions are located on only one of the two daughter arms of the replicating chromosome. Inman and Schnös observed such single-stranded regions in 50% of the growing points of replicating lambda DNA, and, as reported in this paper, we find them in about 85% of the growing points of T7 DNA. Both studies support the conclusion that DNA synthesis involves the direct elongation of one daughter strand in the growing point. Evidently, this elongation is accompanied by the unwinding of the parental double helix to expose a region of single-stranded DNA which is then converted to the duplex state by a discontinuous mechanism involving the synthesis of DNA fragments.


Initiation of DNA synthesis: synthesis of phiX174 replicative form requires RNA synthesis resistant to rifampicin. Conversion of single-stranded DNA of phage varphiX174 to the double-stranded replicative form in Escherichia coli uses enzymes essential for initiation and replication of the host chromosome. These enzymes can now be purified by the assay that this phage system provides. The varphiX174 conversion is distinct from that of M13. The reaction requires different host enzymes and is resistant to rifampicin and streptolydigin, inhibitors of RNA polymerase. However, RNA synthesis is essential for varphiX174 DNA synthesis: the reaction is inhibited by low concentrations of actinomycin D, all four ribonucleoside triphosphates are required, and an average of one phosphodiester bond links DNA to RNA in the isolated double-stranded circles. Thus, we presume that, as in the case of M13, synthesis of a short RNA chain primes the synthesis of a replicative form by DNA polymerase. Initiation of DNA synthesis by RNA priming is a mechanism of wide significance.


In vitro complementation as an assay for new proteins required for bacteriophage T4 DNA replication: purification of the complex specified by T4 genes 44 and 62. We have developed an in vitro complementation assay for six T4 bacteriophage gene products believed to be components of the T4 DNA replication apparatus. This assay is based upon the fact that DNA synthesis in an infected cell lysate that lacks a given gene product is specifically stimulated by addition of the missing product. By the use of such an assay, two proteins that appear to be the products of T4 genes 44 and 62 have been purified to electrophoretic homogeneity as a single complex of the two polypeptide chains.


The use of dopamine  -hydroxylase as a marker for the central noradrenergic nervous system in rat brain. Improvements in the method for localization of dopamine-beta-hydroxylase by immunofluorescence allow the observation of noradrenergic-cell bodies, non-terminal fibers, and axon terminals in the rat brain. The distribution of the hydroxylase correlated well with the results obtained by localization of norepinephrine. Dopamine-beta-hydroxylase was not observed in dopaminergic neurons or terminals, indicating that these cells do not have the capacity to synthesize norepinephrine. The use of the hydroxylase as a marker, however, has made it possible to visualize noradrenergic nerve terminals on small arteries in the brain parenchyma that have not been described by catecholamine-fluorescence histochemistry. The source of the terminals on small arteries appears to be central noradrenergic neurons rather than the peripheral sympathetic nervous system. Dopamine-beta-hydroxylase generally was not observed in the large arteries of the brain parenchyma. These observations suggest that cerebral microcirculation is regulated by central noradrenergic neurons.


Self-association of gene-32 protein of bacteriophage T4. The self-association of gene-32 protein has been studied by sedimentation equilibrium centrifugation and polyacrylamide gel electrophoresis, in order to better understand its role in DNA replication and genetic recombination. The monomer molecular weight of gene-32 protein is 38,000 in guanidine hydrochloride and 34,000 in sodium dodecyl sulfate, in agreement with the results of Alberts and coworkers. Stable dimers of gene-32 protein occur under various conditions, among which are high ionic strength and pH 10. The occurrence of stable dimers under some conditions and higher aggregates under others indicates there are two types of protein-protein interactions occurring in gene-32 protein self-association. The association that occurs above about 0.1 mg/ml concentration of protein produces at least decamers.A model for the DNA replication fork is postulated that requires the two different interactions that occur in gene-32 protein aggregation. In the model, gene-32 protein holds the two strands of the DNA duplex in a conformation that prevents their reannealing and, therefore, facilitates replication and recombination.


Léon Bernard Foundation award.

Léon Bernard Foundation award.


Shousha Foundation award.

Shousha Foundation award.


Some biochemical features of flocculent and non-flocculent yeast used in the top beer brewery in Grodzisk Wlkp.


Attempts at elimination of bacteria from algal cultures by means of antibiotics.


Growth of bacteria in Chlorella vulgaris cultures.


Indirect skeletal fixation.

Indirect skeletal fixation.


Four decades of association with the pioneers of anesthesiology.


Dr. Horace Wells (1815-1848).

Dr. Horace Wells (1815-1848).


Akira Inamoto.

Akira Inamoto.


The influence of chlorpromazine, diazepam, and droperidol on emergence from ketamine.


A world-wide view of venereal disease.

A world-wide view of venereal disease.


Further assessment of rosette inhibition test in clinical organ transplantation. The rosette inhibition test was used in the clinical management of organ allografts to estimate the amount of immunosuppressive drugs necessary to prevent rejection. In patients surviving more than three months renal function appeared to be better than in a similar group of patients managed without the test. It is suggested that this was due to a reduction in the number of clinical or subclinical rejection episodes. On the other hand, the test indicates that in many cases the level of immunosuppression should be much higher, and if this advice is followed the patients become increasingly exposed to the risk of infection. In other words, those patients with good renal function remained well, whereas those who might otherwise have rejected their kidney and survived had in fact died of sepsis.


C. albicans resistance to 5-fluorocytosine.


Immunochemotherapy of cancer with chlorambucil-carrying antibody. Cell-surface localizing heterologous antibodies against the mouse EL4 lymphoma and a human malignant melanoma could be bound to chlorambucil without causing the loss of the alkylating activity of chlorambucil or interfering with the reactivity of the antibodies with their respective tumour cells. When given to mice preinoculated with tumour cells 2, 24, 72, and 120 hours before the beginning of treatment the chlorambucil-bound antibody was a much more effective tumour inhibitor than chlorambucil or the antibody alone. In a patient with disseminated malignant melanoma injection of the chlorambucil-bound anti-melanoma antibody first locally into a few metastatic nodules and then by the intravenous route was followed by the regression of all the metastatic nodules.


Ocular complications in renal transplantation.


Gastric bleeding and benorylate, a new aspirin. Benorylate (4-acetamidophenyl 2-acetoxybenzoate) is a new esterified aspirin preparation whose antirheumatic properties are reported to be as good as those of aspirin. Gastrointestinal blood loss, measured with (51)Cr-labelled red cells, during benorylate therapy was compared with that during therapy with soluble aspirin in 15 subjects, a simplified crossover procedure being used. Mean blood loss during benorylate therapy was 1.7 ml/day which was significantly less than that during therapy with soluble aspirin (5.1 ml/day; P <0.001). In 12 of the 15 patients blood loss with benorylate was less than 2.5 ml/day. Benorylate appears to be a definite improvement on current formulations of aspirin and should be a useful drug for the treatment of patients with chronic rheumatic disorders.


The masticatory performance and dietary intake of elderly edentulous patients.


Bacteriological investigation of dental abscesses.


Studies on the conformation of DNA-dependent RNA polymerase in solution by small-angle x-ray measurements.


Animal DNA-dependent RNA polymerases. 4. Studies on inhibition by rifamycin derivatives.


The secretory immune system of the intestine.


Two types of Zollinger-Ellison syndrome: immunofluorescent, cytochemical and ultrastructural studies of the antral and pancreatic gastrin cells in different clinical states. In this survey the antral, pancreatic and, where present, the neoplastic gastrin cells, were studied in eight cases of the Zollinger-Ellison syndrome. The antral G cells alone were studied in one case of Z-E syndrome, seven cases of simple duodenal ulcer, and five cases of pernicious anaemia. The Z-E cases were divided into two numerically equal groups. The first group had ;short' histories, high serum gastrin levels, and profound antral G cell hyperplasia. The second group had ;long' histories, relatively lower serum gastrin levels, normal antral G cells, and either pancreatic D cell hyperplasia or gastrinoma. Antral G cell hyperplasia, with maximal gastrin storage and normal serum gastrin levels, was found in the duodenal ulcer cases. Antral G cell hyperplasia with minimal storage and high serum gastrin levels was observed in the cases of pernicious anaemia. On the basis of our findings we propose that there exist at least two distinct types (or perhaps stages) of the Z-E syndrome. Suggestions for their pathogenesis are offered.


The specific secondary biological activities of rabbit IgM and IgG anti-Salmonella typhimurium 'O' antibodies isolated during the development of the immune response.


T and B lymphocytes. Organ and age distribution in the chicken.


A further study of electron microscopic particle length of F-actin polymerized in vitro.


Immunoglobulins in bovine mammary secretions. Quantitative changes in early lactation and absorption by the neonatal calf.


Leukocyte migration and lymphocyte transformation tests in renal allotransplantation.


Synthesis of the phosphonic acid isostere of glycerol 3-phosphate.


Nitroheterocyclic antimicrobial agents. 1-Methyl-2-nitro-5-imidazolyl derivatives.


Variety in the response of Escherichia coli to erythromycin.


The production of diarrhoea in baby rabbits by the oral administration of cell-free preparations of enteropathogenic Escherichia coli and Vibrio cholerae: the effect of antisera.


The effect of food and water deprivation (stress) on Salmonella-carrier mice.


The role of thymus-dependent immunity in Mycoplasma pulmonis infections of mice.


Dialysis and ultrafiltration of heat-stable enterotoxin from Escherichia coli.


The nature of the protective effect of antisera against Escherichia coli diarrhoea in piglets.


Fate of ingested Escherichia coli in normal persons.


A phage-inhibitor for the swarming of Proteus.


Correlation between metabolism of phosphatidylglycerol and membrane synthesis in Escherichia coli.


A complex of recombination and repair genes in Haemophilus influenzae.


X-ray crystallographic study of the rennin-like enzyme of Mucor pusillus var. Lindt.


Cell division in Escherichia coli: evidence for regulation of septation by effector molecules.


Metabolic regulation of the tryptophan operon of Escherichia coli: repressor-independent regulation of transcription initiation frequency.


Mutations in the lactose operon caused by bacteriophage Mu.


Transcription initiation frequency and translational yield for the tryptophan operon of Escherichia coli.


Bacteriophage T4 transfer RNA. I. Isolation and characterization of two-phage-coded nonsense suppressors.


Biosynthesis of selenobases in transfer RNA by Escherichia coli.


Testing the hypothesis that a person will be consistent: stylistic consistency versus situational specificity in size of children's drawings.


The distinctiveness of field independence.


Perforation in gastric malignancy.

Perforation in gastric malignancy.


The immunosuppressive activity of procarbazine hydrochloride in canine renal allografts by donor pretreatment.


Biochemical and genetic studies of recombination proficiency in Escherichia coli K12. IV. Analysis of recombinants formed by a recombination deficient (recB21 recC22) strain.


Chromosomal loci for 16S ribosomal RNA in Escherichia coli.


Control of the argECBH cluster in Escherichia coli.


Control regions within the argECBH gene cluster of Escherichia coli K12.


Stereotaxic implantation of radio-isotopes in brain tumours.


Stereotaxic surgery for non parkinsonian dyskinesias.


Hypertension following cadaveric renal transplantation.


Birth order effects: not here, not now.

Birth order effects: not here, not now.


Survival of candida septicaemia treated with amphotericin B.


Occipital alpha revisited.

Occipital alpha revisited.


Note on immediate memory for digits: invariance over the years.


Stimulus compounding in free-operant and classical conditioning. A review and analysis.


The 47,XYY male: a review.

The 47,XYY male: a review.


Some comments on "a behavioral approach to nitrogen narcosis".


A bibliography on the biology and pharmacology of lithium--appendix I.


On the fine structure of the perivascular cells in the neural lobe of rats.


Human toxoplasmosis prevalence and exposure to cats.


The use of commercially available vaccines for the oral vaccination of foxes against rabies.


Presidential address. "Surgery in British Columbia".


Necrotizing fasciitis caused by hemolytic Staphylococcus aureus.


Isopropanol preservation of biological samples for subsequent DNA extraction and reassociation studies.


Magnetic circular dichroism studies. XIX. Determination of the tyrosine: tryptophan ratio in proteins.


Long strands of DNA synthesized in vitro by Escherichia coli DNA polymerase I.


Two fractionation methods for transfer RNAs.


Multiplicity of serine-specific transfer RNAs of brewer's and baker's yeast.


Initiation of transcription and the sequential synthesis of 16-S and 23-S ribosomal RNA in Escherichia coli.


Low molecular RNA associated with chromatin: purification and characterization of RNA that stimulates RNA synthesis.


Protein synthesis with ribonuclease digested ribosomes.


Functional integrity of Escherichia coli 30-S ribosomes reconstituted from RNA and protein: in vitro synthesis of S-adenosylmethionine cleaving enzyme.


Distinction between far- and near-ultraviolet light killing of recombinationless (recA) Salmonella typhimurium.


Transport overshoot during biotin uptake by Saccharomyces cerevisiae.


Transport studies of showdomycin, nucleosides and sugars in Escherichia coli B and in showdomycin-resistant mutants.


Supplementary instruction.

Supplementary instruction.


Clinical evaluation of oxprenolol in angina pectoris.


Studies on the mechanism of shock. Failure to find a role for intestinal bacterial endotoxin in burn shock.


The timing of the response to injury on autografts.


Vesico-ureteric reflux after renal transplantation.


Donor kidney selection for transplantation. Relationships between glomerular structure, vascular supply and age.


Amphotericin B lavage in the treatment of Candidial cystitis.


Arterio-venous shunting occurring with rejection in a renal transplant.


Incomplete duplication of the male urethra. Report of an unusual case.


Nalidixic acid by intravenous infusion. A report of 50 cases.


Ontogeny and localization of  -crystallin antigen in the developing pigeon (Columba livia) lens.


A. G. Signy--editor, Journal of Clinical Pathology, 1944-1972.


Suppressed activity of thymus-derived cell in tumor-bearing host.


Tranexamic acid for prophylaxis in haemophilia.


Fibrinolytic enhancement by stanozolol: a double-blind trial.


Thrombolytic treatment of pulmonary embolism.


Radiographic, haemodynamic and biochemical findings related to the National Heart and Lung Institute's urokinase pulmonary embolism trial.


Drug therapeutic evaluation in acute myocardial infarction.


Current status of thrombolytic agents in acute cerebrovascular disease.


Demonstration of UAG as a nonsense codon in bakers' yeast by amino-acid replacements in iso-1-cytochrome c.


Comparison of the physical and optical properties of Escherichia coli and sea urchin 5 s ribosomal RNA's.


Selective modification of uridine and guanosine residues in tyrosine transfer ribonucleic acid.


Ribosomal binding of bacteriophage RNA with different components of initiation factor F3.


Complex formation with heavy meromyosin of the isolated actin-like component of thrombosthenin, the contractile protein from blood platelets.


Inhibition of RNA bacteriophage replication by rifampicin.


Ribosomal proteins. 33. Location of amino-acid replacements in protein S12 isolated from Escherichia coli mutants resistant to streptomycin.


Dietary carbohydrate and posprandial hyperlipemia.


A plasma protein which increases with vitamin K deficiency.


Ultrastructural studies on iron absorption in normal mouse duodenum.


Effect of unsaturated fatty acid composition on lecithin-cholesterol interactions.


Perspectives in therapeutic pig liver perfusions.


Inhibition of bactericidal capacity in mice after administration of Newcastle disease virus.


Transferable resistance to antibiotics in gram-negative bacteria isolated in a hospital for infectious diseases. I. Occurrence and multiple origin of R factors.


Studies on the absorption of pivampicillin and ampicillin.


Intravascular coagulation in infection: a study by radio-fibrinogen.


Treatment of ventriculitis with gentamicin in an infant born with spina bifida.


Cell culture on artificial capillaries: an approach to tissue growth in vitro. Artificial capillaries perfused with culture medium provide a matrix in which cells can attain tissue-like densities in vitro. Products secreted into the medium can be measured as indicators of cell function or may be recovered for other purposes without disturbing the culture.


The interesting case of furan aspirin.

The interesting case of furan aspirin.


Ultrastructural effect of various buffers, osmolality, and temperature on paraformaldehyde fixation of the formed elements of blood and bone marrow.


The in vivo effect of a 0.1 per cent stannous fluoride mouthwash on various oral microorganisms.


Diseases, decision-makers and doctors.

Diseases, decision-makers and doctors.


The rise of dermatology in Philadelphia.


Etiology, pathology and treatment of chorea in the nineteenth century.


A multiplicity of American pharmacy schools from 1870 to 1900, including a directory.


Chemistry at extinct medical schools of nineteenth-century Philadelphia.


[Memoir of Douglas P. Murphy 1893-1971].


Memoir of J. Howard Pew 1882-1971.

Memoir of J. Howard Pew 1882-1971.


Memoir of Truman Gross Schnabel, Sr. 1886-1971.


V. Leishmaniasis. Epidemiology: transmission.


The rationale of immunostimulation procedures in the therapeutic approach to malignant melanoma of the skin.


In memory of John A.M. Runnström. 17.VI.1888-20.I.1971.


Phospholipid metabolism by phagocytic cells. VI. Observations on the fate of phospholipids of granulocytes and ingested Escherichia coli during phagocytosis.


Phospholipid metabolism by phagocytic cells. VII. The degradation and utilization of phospholipids of various microbial species by rabbit granulocytes.


Keratoconus.

Keratoconus.


Lipid biosynthesis during the recovery of Salmonella typhimurium from thermal injury.


Soil enrichment for the isolation of sporangial subgroup II Bacillus species, and observations concerning a coil-forming member of this group.


Extracellular cephalosporinases produced by gram-negative bacilli.


Closed-loop intestinal obstruction in Candida albicans monocontaminated rats.


A chemically defined medium for Treponema strain PR-7 isolated from the intestine of a pig with swine dysentery.


Ovine ill-thrift in Nova Scotia. 4. The survival at low oxygen partial pressure of fungi isolated from the contents of the ovine rumen.


Effect of hyperbaric atmospheres on  -galactoside transport in Escherichia coli.


Effect of nitrilotriacetic acid on growth and mating in strains of Escherichia coli K-12.


Characterization of a mucoid Escherichia coli K12 strain, and chemical analysis of the exopolysaccharide.


The fine structure and development of chlamydospores of Fusarium oxysporum.


John Alexander McMahon new AHA president.


Index of drinking water pollution--total coliform MPN tests: confirmed test versus completed test.


Ninety-seventh critical bibliography of the History of Science and its cultural influences (to January 1972).


Proliferative characteristics of the ependymal layer during the early development of the spinal cord in the mouse.


Myoepithelial cell differentiation in rat mammary glands.


Morphological analysis of renal polycystosis induced by corticoids.


Angiographic diagnosis of vascular complications in lung transplantation.


Fine structure of the epithelium of the human fetal bladder.


Quantitative derivates of renal radiologic studies. An overview.


Comparative histochemical localization of lysosomal enzymes in mammalian epididymides.


Techniques of data extraction from radiological images. An overview.


The disposition, morphology and innervation of cardiac specialized tissue in the guinea-pig.


Some preliminary observations on the fine structure of decalcified bone.


Subcellular localization of corticotrophin and luteinizing-hormone releasing activity in bovine median eminence.


Further ultrastructural evidence for the maturation of neurohypophysial neurosecretory granules.


The ultrastructure of the collecting ducts in the renal medulla of newborn rats.


Skin window preparations in malignant reticuloses.


ProfessorH. B. Van Dyke, Ph.D., M.D. An appreciation.


Luteinizing hormone releasing factor in ultrafiltrates of blood collected from the pituitary stalk of ovariectomized rats and rats subjected to electrical stimulation of the preoptic area.


What to put in the nebulizer of an IPPB machine.


Effects of pregnancy in the rat on the size and insulin secretory response of the islets of Langerhans.


Electron-microscopic localization of the binding of Escherichia coli RNA polymease to T7 DNA in vitro.


Arrangement of DNA in lambda bacteriophage heads. 3. Location and number of nucleotides cleaved from lambda-DNA by micrococcal nuclease attack on heads.


Terminal repetition in the DNA of bacteriophage T5.


On the conformation of transfer RNA in solution: dependence of denaturation temperature and structural parameters of mixed and formylmethionyl Escherichia coli transfer RNA on sodium ion concentration.


Internal promoter of the tryptophan operon of Escherichia coli is located in a structural gene.


Biogenesis of ribosomes: free ribosomal protein pools in Escherichia coli.


Stereotaxic apparatus and operations in Russia in the 19th century.


Stereotaxic trigeminal tractotomy for post-herpetic facial pain.


Pathological characteristics of experimental thermal lesions. Comparison of induction heating and radiofrequency electrocoagulation.


Teratoma occurring within a myelomeningocele. Case report.


Obituary--Geoffrey Thomas Ashley, B.Sc., M.D.


Accidental administration of syntometrine to a newborn infant.


Patrick Sarsfield Byrne, England's first Professor of General Practice.


Lymphocytic choriomeningitis of the mouse. IV. Depression of the allograft reaction.


Increased carbohydrate content in the thymus and spleen in mice receiving Candida albicans orally (occurrence of lymphomas).


Heart rate and the PR interval.

Heart rate and the PR interval.


Salute to the New York Hospital, 1927 to 1971.


"Polar" Hayes. Arctic journeys of Isaac Israel Hayes, M.D. (1832-1881).


Relief of mixed anxiety-depression in terminal cancer patients. Effect of thioridazine.


Hyperbilirubinemia due to intraperitoneal sepsis.


Medieval medical malpractice and Chaucer's physician.


Topical therapy of burns.

Topical therapy of burns.


A comparison of dextromoramide and papaveretum as postoperative analgesics.


Stream-inoculation of urinary dip-inoculum transport outfit.


Levodopa in parkinsonism.

Levodopa in parkinsonism.


The sanitary state of Brecon in 1849.

The sanitary state of Brecon in 1849.


Segmental demyelination in rheumatoid arthritis.


Body composition in chronic renal failure.


The operative treatment of pressure sores in paraplegics.


Neurosurgical possibilities in the treatment of spastic paresis.


Operative indications on the musculo-skeletal system in cerebral palsy.


Long-term results of upper limb surgery in cerebral palsy.


Operative treatment of the knee joint in cerebral palsy.


Antineoplastic drug effects on spermatogenesis studied by velocity sedimentation cell separation.


The tolerance and safety of intravenously administered benzyl alcohol in methylprednisolone sodium succinate formulations in normal human subjects.


Antilymphocyte serum and the course of rodent malaria in mice.


Cannibalistic large mononuclears in a P. falciparum placenta from a primipars Gambian woman.


Remarkable polymorphonuclear phagocytosis of P. falciparum.


Growth of Plasmodium falciparum through three cycles in vitro by a subculture technique.


Immunofluorescence studies on kidneys of mice infected with rodent malaria.


Fatal case of imported cerebral malaria in the United States.


Synchronous feeding of haematophagous bugs on rabbit lop ears.


The effect of habitat on the prevalence of human scrub typhus in Malaysia.


In vivo and in vitro studies of chloroquine-resistant malaria in West Malaysia.


Felix Marti-Ibanez--1911-1972.

Felix Marti-Ibanez--1911-1972.


Pica: its relation to iron deficiency. A review of the recent literature.


Haemodynamic effects of intermittent positive-pressure ventilation with and without an end-inspiratory pause.


A servo-controlled ventilator measuring expired minute volume, airway flow and pressure.


Affinity chromatography of macromolecules.


Acyl carrier protein.

Acyl carrier protein.


Biochemistry of  -galactosidases.

Biochemistry of  -galactosidases.


The relevance of history for the community mental health approach to children.


A controlled study of the efficacy of pentylenetetrazol (Metrazol) with hard-core hospitalized psychogeriatric patients.


A decade of change in drawings by black children.


A perspective on cardiac transplantation.


Psychiatry in mainland China: additional sources.


Retrograde insufflation of gaseous oxygen into the coronary sinus as a means of myocardial maintenance.


Shapely polysaccharides. The eighth Colworth medal lecture.


Physical properties and subunit structure of L-asparaginase isolated from Erwinia carotovora. 1. l-Asparaginases from Erwinia carotovora and Escherichia coli (EC2 enzyme) are both capable of inhibiting and eliminating certain types of tumour cells. The Er. carotovora enzyme is a more basic protein, however, and in contrast with the EC2 enzyme it contains neither tryptophan nor cystine, and disulphide bonds are therefore absent. The molecule is very stable in solution from pH3.0 to about pH12.0, and is somewhat more stable at alkaline pH than is the Esch. coli enzyme. Calculations based on a s(0) (20,w) 7.43S and a sedimentation-equilibrium molecular weight of 135000+/-10000 give a frictional ratio (f/f(0)) of 1.08. The molecular conformation is therefore very compact in solution, and the electron microscope shows the negatively stained molecules as almost spherical particles with a diameter of 7.2+/-0.7nm. 2. Sedimentation-velocity and equilibrium ultracentrifugation, in 5-8m solutions of urea and guanidinium chloride, and also electrophoresis in sodium dodecyl sulphate-polyacrylamide gel, reveal a dissociation of the native protein molecule into four subunits of similar molecular weight in the range 32500-38000. The enzymically inactive subunits can be physically reassembled into an active tetramer when urea is removed by dialysis. Although the subunit structures of the Er. carotovora enzyme and the Esch. coli enzyme molecules are similar, the secondary bonding forces holding the subunits together in the tetramer are somewhat stronger in the Er. carotovora enzyme. 3. The optical-rotatory-dispersion (o.r.d.) parameters that characterize the Cotton effects arising from ordered structure in the molecule are [m'](233)=-3522+/-74 degrees and [m'](200)=9096+/-1700 degrees . These show very marked changes as the secondary structure is disrupted and the molecule dissociates into subunits. A correlation pathway was traced on the basis of o.r.d. parameters and enzyme activity as the polypeptide chains were denatured and renatured (and reconstituted) into active molecules after the dilution of solutions in urea. Subunits resulting from treatment with sodium dodecyl sulphate do not show the typically disordered o.r.d. profile, but nevertheless they are inactive.


Modification of a stereotaxic guide for trans-nasal pituitary ablation.


Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. The term apoptosis is proposed for a hitherto little recognized mechanism of controlled cell deletion, which appears to play a complementary but opposite role to mitosis in the regulation of animal cell populations. Its morphological features suggest that it is an active, inherently programmed phenomenon, and it has been shown that it can be initiated or inhibited by a variety of environmental stimuli, both physiological and pathological.The structural changes take place in two discrete stages. The first comprises nuclear and cytoplasmic condensation and breaking up of the cell into a number of membrane-bound, ultrastructurally well-preserved fragments. In the second stage these apoptotic bodies are shed from epithelial-lined surfaces or are taken up by other cells, where they undergo a series of changes resembling in vitro autolysis within phagosomes, and are rapidly degraded by lysosomal enzymes derived from the ingesting cells.Apoptosis seems to be involved in cell turnover in many healthy adult tissues and is responsible for focal elimination of cells during normal embryonic development. It occurs spontaneously in untreated malignant neoplasms, and participates in at least some types of therapeutically induced tumour regression. It is implicated in both physiological involution and atrophy of various tissues and organs. It can also be triggered by noxious agents, both in the embryo and adult animal.


Phenotypic properties of a large-cell, radiation-resistant strain of Escherichia coli.


Lactose-utilizing mutants of lac deletion strains of Escherichia coli.


Differentiation between Pseudomonas testosteroni and P. acidovorans by gas chromatography.


Natural and induced bactericidal activities in the hemolymph of the lobster, Homarus americanus: products of hemocyte-plasma interaction.


Adsorption of pesticide by humic substances. A review.


Heterotopic liver transplantation. II. Unfavorable outflow conditions as a possible cause for late graft failure.


Hypotonic duodenography with the use of glucagon.


Ethical standards and clinical research.


Bacterial flora in intraabdominal ileostomy reservoir. A study of 23 patients provided with "continent ileostomy".


Monoplane teeth.

Monoplane teeth.


Clinical evaluation of phentolamine in cardiac arrhythmias.


Radectomy of multirooted teeth.

Radectomy of multirooted teeth.


Influence of the doctor-patient relationship on placebo therapy for patients with myofascial pain-dysfunction (MPD) syndrome.


Sterilization of infected root canals.

Sterilization of infected root canals.


The atrial recovery wave (P T ) studied by averaging computer technique in patients with complete heart block.


A morphological and genetic mapping study of white colony mutants of Streptomyces coelicolor.


The behavior of children in school who were severely malnourished in the first two years of life.


Child bereavement and adult psychiatric disturbance.


Antimicrobial agents from higher plants. I. Introduction, rationale, and methodology.


The Asian species of Strychnos. I. Strychnos as a source of the drug lignum colubrinum (snake-wood).


Rheumatoid arthritis--current thoughts on aetiology and pathogenesis.


Sterile swabs as essential equipment for rapid reliable antibiotic sensitivity tests.


The isolation of spirochaetes from pigs affected with swine dysentery.


The catalase test--a cautionary tale.

The catalase test--a cautionary tale.


A comparison between layered and non-layered horse blood agar plates prepared by a semi-automated process.


A simple method of preparing fungi for microscopy.


Drug therapy 1972. 13. The safety of drugs.


Actions of radiations on human chromosomes.


Results of outpatient treatment of 100 cases of systemic lupus erythematosus.


Dopamine and extrapyramidal motor function and dysfunction.


L-dopa in neurological disease: current status.


List of members: Association for Research in nervous and Mental Disease.


Plain film examination of the spinal canal.


Gas myelography.

Gas myelography.


Myelography of nonmass lesions in the spinal canal.


Myelography in children.

Myelography in children.


Non-specific resistance induced by palmitoylethanolamide.


Toxicity, clearance and distribution of endotoxin in mice as influenced by actinomycin D, cycloheximide,  -amanitin and lead acetate.


Biology of Thiobacillus ferrooxidans in relation to the microbiological leaching of sulphide ores.


The natural history of imprinting.

The natural history of imprinting.


Perceptual entities and forms.

Perceptual entities and forms.


Coherent self-direction and values.

Coherent self-direction and values.


Response uncertainty and autonomic-behavioral integration.


The structure and functional unity of psychological processes.


Cerebral organization and the integration of experience.


Developmental integration in childhood.

Developmental integration in childhood.


Developmental control of three-dimensional polarity in the avian limb.


Functional organization of a vertebrate retina: sharpening up in space and intensity.


Clostridium botulinum type F: isolation from venison jerky. A Clostridium botulinum type F was isolated from the venison jerky responsible for the only type F botulism outbreak reported in the United States. The isolate differed from the prototype Langeland type F strain in being nonproteolytic.


Enteric bacterial growth rates in river water. Enteric bacteria, including stocked strains of pathogenic species and organisms naturally present in the stream, were capable of growth in a chemostat with autoclaved river water taken 750 m below a sewage outfall. Maximal specific growth rates for all organisms occurred at 30 C, whereas culture generation times ranged between 33.3 and 116 hr. Of the six laboratory strains of enteric species used, Escherichia coli and Enterobacter aerogenes grew at generation times of 34.5 and 33.3 hr, respectively, while the remaining Proteus, Arizona, Salmonella, and Shigella spp. reproduced at a rate two to three times slower than the coliforms. Little or no growth occurred in the water at incubation temperatures of 20 and 5 C, and death was observed for Salmonella senftenberg at 20 and 5 C and for E. aerogenes and Proteus rettgeri at 5 C. When enteric bacteria naturally present in the river water were employed in similar experiments, coliform bacteria demonstrated a generation time of approximately 116 hr, whereas fecal coliforms failed to grow. Growth of the bacteria from the river demonstrated a periodicity of approximately 100 hr, which suggests that much of the growth of these organisms in the chemostat may be on the glass surfaces. This phenomenon, however, was not observed with any of the stocked enteric species. Neither the stock cultures nor the aquatic strains were capable of growth in autoclaved river water taken above the sewage outfall at the three temperatures tested.


Fluorometric determination of deoxyribonucleic acid in bacteria with ethidium bromide. A simple, sensitive, and rapid method is presented for the determination of deoxyribonucleic acid (DNA) in both gram-positive and gram-negative bacteria. It is based upon the fluorometric determination of DNA with ethidium bromide after alkaline digestion of the bacteria to hydrolyze the interfering ribonucleic acid. The assay takes less than 2 hr. Its sensitivity is at least 0.2 mug of DNA in a final solution of 4 ml and it uses commonly available filter or double monochromator fluorometers. Judicious choice of light source and filters allows an additional 10-fold increase in sensitivity with a filter fluorometer. Turbidity caused by bacteria or insoluble polysaccharides does not interfere with the fluorescence measurements. There was no significant difference between the results obtained with this method and those obtained with the indole and diphenylamine methods when these assays were applied to Escherichia coli and sucrose- or glucose-grown Streptococcus mutans. The method was also tested by determining the specific growth rate of E. coli. This new procedure should be especially useful for the determination of bacterial DNA in dilute suspensions and for the estimation of bacterial growth or DNA replication where more conventional methods are not applicable or sensitive enough.


Inhibition of fungal growth in the cultural isolation of mycobacteria. Antifungal antibiotics were compared to determine their usefulness in primary mycobacterial cultures. Amphotericin B was found to be more effective in preventing fungal growth from bovine fecal specimens than were cycloheximide, nystatin, and tetracycline. Amphotericin B did not affect the growth rate of the following Mycobacterium species: M. avium, M. bovis, M. intracellulare, M. paratuberculosis, M. phlei, or M. tuberculosis, but it inhibited the growth of M. fortuitum. There was no observable effect on numbers of colonies of M. paratuberculosis on primary isolation from fecal specimens. It is recommended that, for the primary isolation of pathogenic mycobacteria from specimens likely to contain fungi, the inoculum should be pretreated with benzalkonium chloride, followed by mixing with amphotericin B or inoculation onto media containing amphotericin B.


Plating medium pH as a factor in apparent survival of sublethally stressed yeasts. Sublethally stressed cells of 9 of 10 species of yeast were recovered at maximum levels when potato dextrose agar was adjusted to approximately pH 8. The optimum for candida utilis was at approximately pH 10. At pH 3.5, as commonly employed with media selective for yeasts and molds, recovery of heat-stressed organisms ranged from essentially the same as at optimum pH to levels of 1% or less of the maximum count. The extent to which this may be of practical significance in assessing the microbiological quality of food products remains to be determined.


Severe oral lesions in chickens caused by ingestion of dietary fusariotoxin T-2. Fusariotoxin T-2 is a mycotoxin produced by Fusarium tricinctum which was implicated in moldy corn toxicosis of farm animals. Graded concentrations of dietary fusariotoxin T-2 (0, 1, 2, 4, 8, and 16 mug/g, respectively) were given to groups of 40 chickens. Raised yellowish-white lesions on the mouth parts were produced by all concentrations, and the size of the lesions was dose-related. The growth rate was reduced significantly (P < 0.05) by concentrations of 4, 8, and 16 mug/g. The mouth fluid of the affected birds contained greatly increased numbers of bacteria, including Staphylococcus epidermidis and Escherichia coli, which proved avirulent when inoculated into scarified tissue of control birds. Microscopy examinations of the lesions revealed a fibrinous surface layer, intermediate layers containing invaginations filled with rods and cocci, and a heavy infiltration of the underlying tissues with granular leukocytes. These data suggest that the role of fusariotoxin T-2 in field cases of moldy corn toxicosis should be reinvestigated since oral lesions were not mentioned in the original descriptions of the disease. However, the lesions bear some features of those characteristic of the third or septic angina stage of alimentary toxic aleukia, a nutritional toxicosis of humans produced by eating grains infested with F. tricinctum.


Erythritol as a selective substrate for the growth of Serratia marcescens. Serratia marcescens grew on a basal medium containing 0.5% erythritol as sole source of carbon for growth and energy. Only an occasional strain of Enterobacter aerogenes among several members of the family Enterobacteriaceae were able to utilize erythritol.


Repair of injury induced by freezing Escherichia coli as influenced by recovery medium. Freezing an aqueous suspension of Escherichia coli NCSM at -78 C for 10 min, followed by thawing in water at 8 C for 30 min, resulted in the death of approximately 50% of the cells, as determined by their inability to form colonies on Trypticase soy agar containing 0.3% yeast extract (TSYA). Among the survivors, more than 90% of the cells were injured, as they failed to form colonies on TSYA containing 0.1% deoxycholate. Microscope counts and optical density determinations at 600 nm suggested that death from freezing was not due to lysis of the cells. Death and the injury were accompanied by the loss of 260- and 280-nm absorbing materials from the intracellular pool. Injury was reversible as the injured cells repaired in many suitable media. The rate of repair was rapid and maximum in a complex nutrient medium such as Trypticase soy broth supplemented with yeast extract. However, inorganic phosphate, with or without MgSO(4), was able to facilitate repair. Repair in phosphate was dependent on the pH, the temperature, and the concentration of phosphate.


Lack of association between haemophili and human periodontal disease.


The organization of microtubules within rat odontoblast processes revealed by perfusion fixation with glutaraldehyde.


Effect of fluorides on the bonding of resins to phosphoric acid-etched bovine enamel.


The "rotary column" method for growth of large-scale quantities of cell monolayers.


Circulatory studies in clinical hyaline membrane disease.


Leucocyte ascorbic acid and pressure sores in paraplegia.


Sir Rowan Boland.

Sir Rowan Boland.


P. B. Mumford.

P. B. Mumford.


Sir Frank P. Montgomery.

Sir Frank P. Montgomery.


First isolation of Corynebacterium suis in Hong Kong.


Hemodynamics of hypertension in chronic end-stage renal disease.


Clinical and hemodynamic results of intraaortic balloon pumping and surgery for cardiogenic shock.


Systolic time intervals as measures of the contractile state of the left ventricular myocardium in man.


Effects of drugs on clinical laboratory tests.


Flavoxate (Urispas).

Flavoxate (Urispas).


Perphenazine action on vestibular neurons.


Vitreoretinal juncture; topographical variations.


Electron microscopic studies of the elastic fiber in human sclera.


Lymphocytic choriomeningitis virus-induced retinopathy in newborn rats.


A dream of the Marquis de Sade: psychoanalytic reflections on narcissistic trauma, decompensation, and the reconstitution of a delusional self.


Bertha Pappenheim's pathological mourning: possible effects of childhood sibling loss.


Antibiotics. An evaluation of a buffered agar method for the assay of neomycin sulfate in feeds.


Lymphopoiesis in the rat. I. The effect of pool size on lymphocyte production.


A fine-structural study of conidium initiation in Stemphylium botryosum Wallroth.


Real and reflected images of cells in profile. I. A method for the study of cell movement and adhesion.


Bioinorganic chemistry. Simple models of iron sites in some biological systems.


Effects of storage on CFU of mouse bone marrow cells.


An epidemiological study of strains of Shigella sonnei from two related outbreaks. During the winter of 1967-8 Sonne dysentery affected neighbouring North London primary schools. This was not simply due to cross-infection between the two schools, for two different, unusual strains of Sh. sonnei were distinguished. One was a novel kanamycin-resistant colicine type 7 strain, and as far as we know this was the first school outbreak due to such a strain to be documented. The other strain was kanamycin-sensitive and of colicine type 0 with a rare specific requirement for aspartic acid. There was some evidence to suggest that the kanamycin-resistant strain was more infective for adults and possibly more pathogenic than the kanamycin-sensitive.Studies on the transfer of drug resistance and of colicinogeny revealed that the factor determining colicine type 7 was carried on a transmissible plasmid, a new observation. Various drug resistances were also transmissible experimentally, and some were spontaneously unstable. Non-transferable ampicillin-resistance in the colicine type 7 strain and aspartic acid dependence in the colicine type 0 strain enabled all but one of the isolates to be classified into two distinct lines. No common ancestor was found and it was concluded that although occurring together they must have arisen from separate sources.


[Thermosensitive structure in spinal cord].


Radiobiological studies on cultured mouse bone marrow cells in vitro.


Effect of electrical stimulation of the hypothalamus on plasma levels of free fatty acids and glucose in rats.


Treatment of ovarian carcinoma: possibilities for progress.


Some aspects of the use of small needle-shaped semiconductor detectors in the determination of regional distribution and transport of labelled compounds.


Group trial of Althesin as an intravenous anaesthetic.


A comparison of recovery times between Althesin and methohexitone following anaesthesia for electro-convulsive therapy.


Some aspects of the clinical pharmacology of Althesin.


Maintenance schedules and hunger drive: an examination of the rat literature.


Progression and remission in the nehprotic syndrome associated with quartan malaria in Uganda.


Renal disease in partial lipodystrophy.

Renal disease in partial lipodystrophy.


Effect of Gymnodinium breve toxin on hemolysis induced by Prymnesium parvum toxin.


Effect of scorpion venom on ultrastructure of frog sartorius muscle.


The effect of neonatal androgen on the activity of certain enzymes in the rat hypothalamus.


Urinary tract infections in association with radium therapy for gynecological cancer.


An annotated bibliography of the British Nycteribiidae.


Fibrinolytic activity of veins during treatment with medroxyprogesterone acetate.


Subunit interactions in aspartate transcarbamylase.


Sotalol for the protection of turkeys from the development of  -aminopropionitrile-induced aortic ruptures. 1. The influence of feeding 2 levels of sotalol on the incidence of beta-aminopropionitrile (BAPN)-induced aortic ruptures of immature turkeys was determined.2. Four of 22 turkeys fed 0.12% sotalol and 0.07% BAPN died of aortic ruptures, but 6 of 21 turkeys fed only BAPN died of the syndrome.3. Blood pressure, heart rate, aortic tensile strength, and aortic structure as seen by light- and electron-microscope were similar in turkeys fed BAPN alone or both BAPN and 0.12% sotalol concurrently.4. In a second experiment, 13 of 24 turkeys fed 0.7% BAPN alone died of aortic ruptures, but only 2 of 24 turkeys fed BAPN and 0.2% sotalol concurrently died of the disease.5. Aortic tensile strength was lower, heart rate was faster, alterations of aortic elastic fibres as seen by light- and electron-microscope were more severe, and aortic salt soluble collagen with a higher amino acid content was increased in turkeys fed only BAPN, as compared to turkeys fed both BAPN and 0.2% sotalol.


Isolation and partial characterization of the cyanogen bromide fragments of   1 -acid glycoprotein and the elucidation of the amino acid sequence of the carboxyl-terminal cyanogen bromide fragment.


Psychiatry in Latin America.

Psychiatry in Latin America.


General paresis in the psychiatric department of a general hospital in India.


"Senile dementia": a changing perspective.


In vitro sensitization to transplantation antigens. IV. Density gradient centrifugation of sensitized cells and sensitization of density gradient-fractionated cells.


Short communication. T cells. The limiting cells in the initiation of immune responses in normal mouse spleens.


Review. Antigen--RNA interactions.

Review. Antigen--RNA interactions.


Physiological changes during operations on the limbic system in man.


Carcinoid tumors of the colon (exclusive of the rectum): review of the literature.


A radioimmunoassay for cortisol in plasma and urine.


Clinical evaluation in men of a substance with growth hormone-releasing activity.


Specific nuclear triiodothyronine binding sites in rat liver and kidney.


An investigation of the clinical effect of GPA-1714, a catechol-O-methyl transferase inhibitor.


Normal and pathological conditions which may alter the human requirement for vitamin B 6 .


Tableting research and technology.

Tableting research and technology.


Evaluation of bensulide for mutagenic properties in microbial test system.


Evaluation of herbicides for possible mutagenic properties.


Plasmid-controlled variation in the content of methylated bases in bacteriophage lambda deoxyribonucleic acid. The N(6)-methyladenine (MeAde) and 5-methylcytosine (MeC) contents in deoxyribonucleic acid (DNA) of bacteriophage lambda has been analyzed as a function of host specificity. The following facts have emerged: (i) lambda grown on strains harboring the P1 prophage contain ca. 70 more MeAde residues/DNA molecule than lambda grown either in the P1-sensitive parent, or in a P1 immune-defective lysogen which does not confer P1 modification; (ii) lambda grown on strains harboring the N-3 drug-resistance factor contain ca. 60 more MeC residues/DNA molecule than lambda grown on the parental strain lacking the factor; (iii) lambda grown in Escherichia coli B strains is devoid of MeC, whereas lambda grown in a B (N-3) host contains a high level of MeC; (iv) the MeAde content in lambda DNA is not affected by the N-3 factor. These results suggest that P1 controls an adenine-specific DNA methylase, and that the N-3 plasmid controls a cytosine-specific DNA methylase. The N-3 factor has been observed previously to direct cytosine-specific methylation of phage P22 DNA and E. coli B DNA in vivo; in vitro studies presented here demonstrate this activity.


Filamentous bacterial viruses. IV. Fate of the infecting parental single-stranded deoxyribonucleic acid. Some of the infecting fd single-stranded viral deoxyribonucleic acid (DNA) molecules were found to be degraded after having initiated infection. The degradation products were reused for synthesis of DNA, primarily bacterial. Degradation was most extensive when viral DNA was replicating.


Filamentous bacterial viruses. V. Asymmetric replication of fd duplex deoxyribonucleic acid. Short pulses (30 sec at 32 C) of (3)H-thymidine were found primarily in the viral strands of replicating fd deoxyribonucleic acid (DNA), even at a time when most DNA being synthesized was duplex DNA. Much of the labeled viral strand DNA was longer than unit length, but some was shorter than unit length. Most of the corresponding complementary-strand DNA was recovered in closed supercoiled duplex molecules, even for short pulses; the remainder of the complementary-strand DNA was found in replicative intermediates in pieces shorter than unit length. Some of the viral strands in open replicating DNA lacked a corresponding complementary strand.


Filamentous bacterial viruses. VI. Role of fd gene 2 in deoxyribonucleic acid replication. Functional gene 2 product was found to be necessary for fd deoxyribonucleic acid (DNA) synthesis throughout the life cycle of the virus. Bacteria which had been infected with a temperature-sensitive gene 2 mutant ceased to make virus-specific DNA when transferred to restrictive conditions at any time after infection, although current rounds of replication were completed.


Bacteriophage T4 head morphogenesis. IV. Comparison of gene 16-, 17-, and 49-defective head structures. Defective heads present in extracts of bacteriophage T4 gene 16, 17, or 49 mutant-infected cells have been characterized. All appeared as empty shells when examined by negative-stain electron microscopy and showed essentially the same polypeptide pattern on sodium dodecyl sulfate-acrylamide gels. However, when analyzed by several other methods, gene 16- and 17-defective heads were shown to differ markedly from phage heads present in gene 49-defective extracts. First, the gene 16- and 17-defective structures were found to possess a large number of attached tails (50%, rather than about 5%). Second, they contained less nuclease-resistant deoxyribonucleic acid (DNA) (3 versus 18% of a phage equivalent), had a smaller sedimentation coefficient (240 versus 315S), and a lighter density (1.31 vs. 1.34 g/ml) than gene 49-defective heads. Third, they were not attached to the intracellular DNA pool through a deoxyribonuclease-sensitive linkage. Finally, 8-nm diameter capsomers were clearly revealed on the surface of many gene 16- and 17-defective structures. There was a total of 305 +/- 25 capsomers per particle, which yielded an approximate molecular weight of 84 x 10(6) for these heads. The capsomers were presumably not seen on gene 49-defective heads because of the large amount (18%) of associated DNA. These results support the contention that gene 16- and 17-defective heads, in contrast to gene 49-defective structures, represent abortive rather than intermediate structures in T4 head morphogenesis.


Change in methylation of Salmonella bacteriophage P3 deoxyribonucleic acid with host-controlled modification by Escherichia coli. Modification of bacteriophage P3 by passage through Escherichia coli K was correlated with a 54% decrease in the content of 6-methylaminopurine in the phage deoxyribonucleic acid.