Avian Salmonella-stained microtest antigens produced on solid media. Procedures are described for the preparation of Salmonella pullorum and Salmonella typhimurium tetrazolium-stained microagglutination and microantiglobulin antigens with solid media for culture propagation. Cell suspensions were found to be more easily harvested from solid medium than from previously used liquid medium, and greater yields of antigen were obtained. Liquid medium microagglutination and microantiglobulin test antigens were found to be more sensitive than those prepared on solid medium. The microtest antigens produced on solid medium were easier to prepare and in serological tests gave titers more comparable to those demonstrated with the standard macroscopic tube agglutination test.


Effect of sodium nitrite and sodium nitrate on botulinal toxin production and nitrosamine formation in wieners. Wieners were formulated and processed approximating commercial conditions as closely as possible. Twenty-four batches of product were made with the addition of six levels of sodium nitrite (0, 50, 100, 150, 200, and 300 mug/g), four levels of sodium nitrate (0, 50, 150, and 450 mug/g), and two levels of Clostridium botulinum (0 and 620 spores/g). After formulation, processing, and vacuum packaging, portions of each batch were incubated at 27 C or held for 21 days at 7 C followed by incubation at 27 C for 56 days. The latter storage condition approximated distribution of product through commercial channels and potential temperature abuse at the consumer level. Samples were analyzed for botulinal toxin, nitrite, and nitrate levels after 3, 7, 14, 21, 28, and 56 days of incubation. When nitrite was not added, toxic samples were detected after 14 days of incubation at 27 C. At the lowest level of nitrite added (50 mug/g), no toxic samples were observed until 56 days of incubation. Higher levels of nitrite completely inhibited toxin production throughout the incubation period. Nine uninoculated samples, representing various levels and combinations of nitrite and nitrate, were evaluated organoleptically. The flavor quality of wieners made with nitrite was judged significantly higher (P = 0.05) than of wieners made without nitrite. The nine samples were negative for 14 volatile nitrosamines at a sensitivity level of 10 ng/g. The results indicated that nitrite effectively inhibited botulinal toxin formation at commercially employed levels in wieners and that detectable quantities of nitrosamines were not produced during preparation and processing of the product for consumption.


Needless retesting of quality-assured, commercially prepared culture media. Current revival of interest in quality control of bacteriological culture media prompted this laboratory to develop and implement a system of quality control testing of media purchased from commercial manufacturers. During 8 months we tested more than 900 lots of 46 different media representing 350,000 units of culture media purchased from two major and one minor supplier(s). Only 17 lots were found to be unsatisfactory. This experience raised a question about the real necessity for extensive retesting by users of commercially prepared, quality-assured media. It is suggested that primary responsibility for quality control be placed on the relatively few manufacturer-vendors rather than on the multiple purchaser-users, who may not possess either the expertise or the resources for quality control programs.


Tryptophol formation by Zygosaccharomyces priorianus. Zygosaccharomyces priorianus converted L-tryptophan to tryptophol and to small quantities of indole-3-acetic acid. Neither tryptophol nor indole-3-acetic acid was metabolized when added separately to growing cultures. The possible intermediacy of indole-3-pyruvic acid, indole-3-acetaldehyde, and tryptamine in the degradation of L-tryptophan was tested by feeding these compounds to Z. priorianus and Saccharomyces cerevisiae. Indole-3-pyruvic acid and indole-3-acetaldehyde were converted to tryptophol and indole-3-acetic acid, with the latter accumulating only in small amounts. Tryptamine was converted to its N-acetyl derivative by these organisms. A qualitative study was made on the metabolism of L-phenylalanine, L-tyrosine, and L-5-hydroxytryptophan by these organisms. Like L-tryptophan, these amino acids were metabolized to their respective alcohol and acid derivatives. Of a large number of organisms tested, the yeasts possessed the highest capacity for degrading L-tryptophan to tryptophol.


Rapid detection of gram-negative bacteriuria by use of the Limulus endotoxin assay. The Limulus in vitro endotoxin assay was evaluated as a possible method for the prompt detection of significant gram-negative bacteriuria in children. This assay is capable of detecting endotoxin associated with intact cell walls of viable gram-negative bacteria as well as free endotoxin. Quantitative results are obtained following a 1-h incubation of Limulus lysate and 10-fold dilutions of otherwise untreated urine. A standard curve of Limulus activity and viable cell counts of Escherichia coli and Klebsiella pneumoniae in urine demonstrated that a positive Limulus reaction at a dilution of 1:100 or 1:1,000 indicated a colony count of at least 100,000 bacteria/ml. A positive Limulus reaction only from undiluted urine or at a dilution of 1:10 indicated less than 100,000 cells/ml. These experimental observations were confirmed by comparing the Limulus test with quantitative plate counts on 209 urine specimens from a mixed pediatric population. These results indicate that the Limulus assay is a simple, accurate method for rapid presumptive detection of gram-negative bacteriuria in patients where an immediate diagnosis is needed. This test would also seem promising for screening large patient populations for bacteriuria or for monitoring the effectiveness of treatment of urinary tract infections.


Intraoperative histological examination today.


An enzymatic-isotopic microassay for putrescine.


Partial purification of ribosomal RNA(m1G)- and rRNA(m2G)-methylases from Escherichia coli and demonstration of some proteins affecting their apparent activity.


Accentuated type I reversibility of maize DNA denaturation thermal stability conferred by guanine-cytosine-rich tracts.


Formation in vitro of 1-methylguanine in 23-s RNA from Escherichia coli. The effects of spermidine and two proteins.


Endonuclease-sensitive sites in the DNA of irradiated bacteria: a rapid and sensitive assay.


Reaction between DNA and N-hydroxyurethan.


Incorporation of 5-substituted uracil derivatives into nucleic acids. II. Incorporation of 5-vinyluracil into the DNA of Escherichia coli.


Mycosis fungoides.

Mycosis fungoides.


Nuclear and mitochondrial DNA synthesis in gamma ray-resistant and -sensitive slime mold amebas. Uptake of [methyl-(3)H]thymidine label from Escherichia coli 15T(-) into the DNA of Dictyostelium discoideum has been measured in control and [(60)Co]-gamma-irradiated cells of the resistant strain NC-4 (D(10), colony-forming survival = 300 krad) and two sensitive daughter strains, gammas-18 (D(10) = 75 krad) and gammas-13 (D(10) = 4 krad). Nuclear (n) and mitochondrial (m) DNA were resolved by isopycnic CsCl gradients. The uptake of label into n-DNA during the immediate postirradiation period was selectively inhibited by irradiation, compared with uptake into m-DNA. For all three strains, the gamma ray dose to reduce the uptake into n-DNA to 37% of the control during the first hour after irradiation was 3 krad, whereas for uptake into m-DNA it was 75 krad. After the initial dose- and strain-dependent lag, uptake into n-DNA resumed. gammas-18 showed longer lags in n-DNA synthesis and cell division than did NC-4. gammas-13 resumed n-DNA synthesis and cell division after slightly shorter lags than for NC-4. The early postlag uptake into n-DNA in this strain was almost at the control rate and was accompanied by division until the cell number had nearly doubled. The rate of label uptake then declined, division stopped, and gradual cell lysis ensued. The postdelay response of gammas-13 was almost independent of dose in the range of 10-100 krad. The response of gammas-18 in these and earlier experiments is consistent with the view-point that it is sensitive because of a decreased rate of repair of DNA damage. However, the basis for the sensitivity of gammas-13 seems to be more complex. This strain undergoes a premature but short-lived burst of n-DNA synthesis and division for what appears to be about one round of replication. Replication then ceases, even at very low doses, leading to greatly reduced probability of survival.


A double-blind trial of oral gefarnate in duodenal ulcer.


Preliminary clinical trial of fusidic acid in psoriasis.


Skin complications in immunosuppressed patients: follow-up of kidney recipients.


Chronic muco-cutaneous candidiasis: new approaches to treatment.


The blastic crisis of chronic myeloid leukaemia: acute transformation of a preleukaemic condition?


Arizona State Dental Association 1973-74 roster.


Application of immunological techniques to the study of classic haemophilia and von Willebrand's disease.


Immunohistological localization of coagulation factors on vascular intima.


Suction drainage in breast surgery.

Suction drainage in breast surgery.


Subclinical infection in leprosy.

Subclinical infection in leprosy. Lymphocyte transformation has been used to study the immune response to Mycobacterium leprae among contacts and non-contacts of leprosy patients. Of 26 subjects living in a leprosy endemic area for less than two months none responded to M. leprae; 24% of subjects who had lived in an endemic area for more than a year gave a positive response to M. leprae; more than 50% of individuals with occupational contact of leprosy for more than a year responded; and about 50% of contacts of tuberculoid and treated lepromatous patients responded to M. leprae, while only 22% (4/18) of contacts of lepromatous patients treated for less than six months responded.It seems that leprosy is more highly infectious than is indicated by the prevalence of the disease and that a subclinical infection commonly follows exposure to M. leprae. The relatively low response found in contacts of active lepromatous patients suggests that in these contacts "superexposure" to M. leprae can bring about a decrease in host resistance.


St. John Ambulance Air Wing.

St. John Ambulance Air Wing.


Clinical diagnostic process: an analysis. An analysis of observations made during 1,307 diagnoses by a total of 28 clinicians (503 diagnoses in real life, and 804 on simulated patients) concerned primarily the interview of patients suffering from abdominal pain. Interviews ranged from 10 to 35 questions, and from "stereotyped" procedures, in which identical (and often irrelevant) questions were asked to each patient, to "adaptive" interviews, in which specific relevant questions were put to each patient. Senior clinicians tended to ask fewer, more relevant questions than their junior counterparts; and urgent cases were dealt with in a more adaptive fashion than routine cases in outpatients. Disappointingly, there was considerable difference between real-life and simulated situations. From these results it is suggested (a) that the "diagnostic process" does not exist, (b) that any automated diagnostic system must be flexible to accommodate the wishes of a variety of clinicians, and (c) that studies based on artificial clinical situations should be treated with extreme caution.


T.L. de Courcy.

T.L. de Courcy.


Aspirin in prophylaxis of ischemic heart disease.


H.A.H. Selbourne.

H.A.H. Selbourne.


P.E.F. Frossard.

P.E.F. Frossard.


Cincinnati Dental Society 1973 directory.


Finger tip amputations. Review of procedures and applications. Repair of finger tip amputations depends upon the slope of transsection and how much of the tip has been amputated. Type 1 and 2 injuries are easily handled in the emergency room by local flaps with results acceptable by functional and economic criteria. Type 3 amputations with losses of less than 25 percent can be repaired by primary closure. Losses of 50 percent or over are best treated by local or "distant" flaps from the involved or adjacent fingers or palm. Each style of flap and technique has advantages and disadvantages.


A false-positive FTA-ABS test in a heroin addict.


The early workers in clinical radiotherapy of cancer at the Radium Institute of the Curie Foundation, Paris, France.


Phagocytic, opsonic and immunoglobulin studies in newborns. Despite improved diagnositc and therapeutic measures, neonatal septicemia continues to be a major clinical problem. Improvement in the management of the septic neonate should result from application of the increasing information being learned of the neonatal host defense mechanisms. The earlier concept that the neonate was "immunologically null" can now be discarded. Evidence summarized in this review has established that the neonate can marshal an immune response to most antigens and infectious agents.A further major advance in recent years has been in understanding of the inflammatory response in the neonate, which includes such activities as chemotaxis, phagocytosis and bactericidal killing of ingested bacteria by phagocytes.


Non-Hodgkin's lymphomas. V. Results of radiotherapy.


Aging today: a review.

Aging today: a review.


Kidney transplantation at Hartford Hospital.


Surgeon Bentley and the first human blood transfusion.


The enzyme theory and the origin of biochemistry.


William Charles Wells and the races of man.


Pre-prosthetic considerations--aberrant frena and muscle attachments.


Routine operation of an Elliott 903 computer in a clinical chemistry laboratory. Experience gained in the last four years concerning the capabilities and limitations of an 8K Elliott 903 (18-bit word) computer with magnetic tape backing store in the routine operation of a clinical chemistry laboratory is described. Designed as a total system, routine operation has latterly had to be confined to data acquisition and process control functions, due primarily to limitations imposed by the choice of hardware early in the project. In this final report of a partially successful experiment the opportunity is taken to review mistakes made, especially at the start of the project, to warn potential computer users of pitfalls to be avoided.


The relationship between the biotype of Klebsiella species and their pathogenicity. Using the biochemical scheme of Cowan, Steel, Shaw, and Duguid (1960) 209 strains of the genus Klebsiella isolated from sputum were classified and their relationship to disease in the patients from whom they were isolated was examined. K. aerogenes predominated and was seldom of proven pathogenicity. K. ozaenae was the next most common species isolated and was of pathological significance in 38% of cases. K. edwardsii var edwardsii, K. edwardsii var atlantae, and K. pneumoniae were less commonly isolated but were more frequently associated with severe disease than K. aerogenes and K. ozaenae.


Circular and branched circular concatenates as possible intermediates in bacteriophage T4 DNA replication.


The effect of temperature of the culture medium on the outcome of blood culture. Small numbers of seven species of bacteria commonly causing bacteraemia in man were inoculated into fresh human blood, and then cultured by a standard method used for diagnostic blood culture. Prior warming of the medium did not improve the recovery of the inoculated bacteria and it is suggested that such warming is not required when performing diagnostic blood culture.


In vivo transcription patterns of temperate coliphage P2.


Temporomandibular joint ankylosis: the rationale for grafting in the young patient.


Ovum development and transfer.

Ovum development and transfer.


Factors involved in the failure of sperm transport and survival in the female reproductive tract.


Biochemistry of the developing embryo.

Biochemistry of the developing embryo.


Prostaglandins and reproduction: a general survey.


Endocrinology of implantation.

Endocrinology of implantation.


The role of prostaglandins in luteal regression.


Pituitary and ovarian mechanisms in implantation.


Control of epididymal function.

Control of epididymal function.


Polypeptide antagonists of the hypothalamic luteinizing hormone releasing factors.


Sperm transport and storage in vertebrates.


Lipid metabolism of spermatozoa.

Lipid metabolism of spermatozoa.


Physiological aspects of human ovulation, fertilization and cleavage.


Tennessee Dental Association directory and membership roster 1973-74.


Serratia marcescens septicaemia in the dog.


The role of DNA polymerase I and the rec system in survival of bacteria and bacteriophages damaged by the photodynamic action of acridine orange.


Mechanism of repression of methionine biosynthesis in Escherichia coli. II. The effect of metJ mutations on the free amino acid pool.


Mechanism of repression of methionine biosynthesis in Escherichia coli. I. The role of methionine, s-adenosylmethionine, and methionyl-transfer ribonucleic acid in repression.


Mechanism of genetic recombination during bacterial conjugation of Escherichia coli K 12. IV. Heterogeneity of progeny of exconjugants. Role of donor and recipient strains.


The role of c3 product and anti-immunity in zygotic induction of bacteriophage lambda.


Henoch's purpura. Eduard Heinrich Henoch (1820-1910).


Spina bifida in general practice.

Spina bifida in general practice.


Beclomethasone dipropionate used as an aerosol in the treatment of asthma.


The action of tetranicotinoylfructose in peripheral vascular disease.


Gentamicin-hydrocortisone ear-drops in chronic infections.


Some historical aspects of chest pain.

Some historical aspects of chest pain.


Tinidazole in amoebiasis.

Tinidazole in amoebiasis.


The many uses of the Omnivac precision vacuum adapter.


Resin bonding to acid-etched tooth surfaces.


Mumps virus recovered from testicles by fine-needle aspiration biopsy in cases of mumps orchitis.


Antibiotic resistance and R factors in gram-negative bacteria isolated in a hospital for infectious diseases. IV. Influence of hospital environment on the incidence and type of resistance in enteric bacteria.


Antibiotic resistance and R factors in gram-negative bacteria isolated in a hospital for infectious diseases. 3. The effect of antibacterial treatment on the incidence of R factor-mediated antibiotic resistance.


Treatment of urinary tract infections with a sulphamethoxazole-trimethoprim compound: a controlled, double blind, clinical trial.


The examination of cerebrospinal fluid cells by fluorescent antibody staining to detect mumps antigen.


Treatment of urinary tract infection with pivampicillin. A new ampicillin derivative.


Historical-demographic analysis of Indian populations in Tlaxcala, Mexico.


Effect of synthetic luteinizing hormone- and follicle stimulating hormone-releasing hormone (LH-FSH-RH) on serum levels of LH, FSH, thyrotrophin (TSH) and growth hormone (HGH) in normal male subjects.


Studies on sustained contraceptive effects with subcutaneous polydimethylsiloxane implants. 3. Factors affecting steroid diffusion in vivo and in vitro.


Relations between blood flow, arterial cross sectional area and total and cortical volumes of the kidney.


Renal blood flow in mobile kidneys measured by a dye dilution technique in combination with angiography.


The influence of preoperative pension on vocational rehabilitation following renal transplantation.


The action of perhexiline maleate in patients with angina.


A 90Sr--90Y applicator for extracorporeal blood irradiation.


Anodal stimulation as a cause of pacemaker-induced ventricular fibrillation.


Clinical experiences with preservation of necrokidneys. The effect of pretreatment with chlorpromazine and the use of a perfusate which mimics the intracellular ion composition.


Terminal renal failure.

Terminal renal failure.


Assessment of prognosis in acute myocardial infarction by computer analysis of cardio-respiratory variables.


Recent advances in venereology. II. Gonorrhoea and other sexually-transmitted diseases.


Causes and duration of renal failure and duration of working disability in dialysis candidates. A retrospective study of 104 patients.


The dermatotrophic activity of nandrolone decanoate in eczema.


Alclofenac in rheumatoid arthritis: an evaluation of its anti-inflammatory and analgesic effects.


Molecular configuration and states of aggregation of biopolymers.


Folding of nucleic acids.

Folding of nucleic acids.


Assembly in biological systems.

Assembly in biological systems.


Interactions between synthetic polymer molecules.


Polymerization in biological systems. Introduction: the objectives.


Protein interactions in the myofibril.

Protein interactions in the myofibril.


The lac repressor and the lac operator.

The lac repressor and the lac operator.


Polymerization in biological systems. Chairman's summary.


On the function of DNA-dependent RNA polymerase.


Protein synthesis machinery and the regulation of messenger RNA translation.


Synthetic polymers, biopolymers and block polymers.


Bacterial cell wall biosynthesis.

Bacterial cell wall biosynthesis.


Circulatory reflexes from mechanoreceptors in the cardio-aortic area.


Studies on the coronary circulation during rejection of the heterotopically transplanted dog heart.


Planning partial denture construction.

Planning partial denture construction.


Comparison of base metal alloys and Type IV gold alloys for removable partial denture frameworks.


RPI (rest, proximal plate, I bar) clasp retainer and its modifications.


The combination clasp and the distal extension removable partial denture.


Major connectors, united it stands.

Major connectors, united it stands.


The influence of the temporary prosthesis and modified restorative techniques in preventive partial dentures.


Principles, planning, and practice for prevention.


Intracoronal attachments.

Intracoronal attachments.


Reduction of residual ridges in the partially edentulous patient.


The overdenture.

The overdenture.


Fluorescence polarization immunoassay. Theory and experimental method.


Cellular and humoral effects of hyposensitization in patients with summer hay fever.


Specific antibodies in allergic subjects.


The behaviour of complement and platelets in lethal endotoxin shock in rabbits.


Dynamics of immune complex nephritis during antibody excess.


The first Carl Prausnitz memorial lecture.


Immunological studies in coeliac disease.


Diagnosis of allergic drug reaction.

Diagnosis of allergic drug reaction.


Studies of mononuclear cells from inflammatory joint effusions.


Quantitation of IgE antibodies and allergens by the radioallergosorbent test, RAST.


Standardization of diagnostic work in allergy.


Potential antimalarial agents. IV. Derivatives of 2-chloro-4'-(p-nitrophenylsulfonyl) acetanilide.


The in vivo and in vitro potentiation of sulfamethoxazole by trimethoprim against strains of Serratia marcescens.


Orthodontic management of tipped abutment molars.


The effect of an alexidine mouthwash on human plaque and gingivitis.


Surgical repositioning of the posterior maxillary segment as an aid to fixed prosthodontics.


Comparative study of bone marrow and blood B cells in infantile and acquired agammaglobulinemia. Possible role of circulating anti-IgM in pathogenesis. The status of immunoglobulin (Ig) receptors of the bone marrow dependent (B) cells present in either the bone marrow (BM) or peripheral blood (PB) of three patients with infantile agammaglobulinemia (I-AGG), or seven patients with acquired agammaglobulinemia (A-AGG) is compared with those of 12 controls. Quantitative and qualitative changes of the different classes of Ig receptors on B cells were evaluated by their capacity to bind [(125)I]anti-Ig, to be stained with fluorescinated anti-Ig and their in vitro proliferative capacity upon incubation with the anti-Ig. Patients with I-AGG lacked B cells in both the BM and PB. Whereas BM cells of patients with A-AGG carried receptors similar to control cells, their blood B cells had fewer IgM, IgG, and IgA cells which failed to proliferate in vitro in the presence of the anti-Ig. An anti-IgM of the IgG class was detected in the sera of patients with A-AGG but not in sera of I-AGG. The isolated anti-IgM agglutinated human red cells coated with IgM. The anti-IgM partially blocked the binding of fluorescinated or radiolabeled anti-IgM to IgM peripheral blood lymphocytes of normal controls. The eluted anti-IgM in presence of complement was partially cytotoxic to normal cells. It is concluded that I-AGG-B cell defect is due to failure of B cell development in the bone marrow compartment whereas the peripheral exclusion of IgM cells by an anti-IgM with the subsequent failure of differentiation of both IgG and IgA cells could be an important mechanism in A-AGG-B cell defect.


The Lancet: aet. CL--gaudeamus igitur.

The Lancet: aet. CL--gaudeamus igitur.


Change at JAMA.

Change at JAMA.


Lung clearance in two patients with cystic fibrosis.


The barracks at Scutari: start of a nursing legend.


Use of a fluorescent antibody technique for the serological differentiation of leptospiral serotypes in cultures and in bovine urine.


Indications for the use of mechanical ventilation in COPD.


The role of surgery in COPD.

The role of surgery in COPD.


Posttransfusion malaria in a newborn.

Posttransfusion malaria in a newborn.


Sites and mechanisms of airway obstruction in COPD.


Sarcoid.

Sarcoid.


A radiologic approach to hypersensitivity pneumonias.


Infective endocarditis. Roentgenographic considerations.


The sickness of Frederic Chopin. A study of disease and society.


Clinical notes of Dr. Jean Baptiste Bucquet (1771-1841).


Oxalate metabolism. 3.

Oxalate metabolism. 3.


McCollum Award. Victor D. Herbert, M.D.

McCollum Award. Victor D. Herbert, M.D.


Nutrition and endurance.

Nutrition and endurance.


Evaluation of the clinical importance of anemia.


The use of lithium in affective disorders. 3. A double-blind study of prophylaxis in bipolar illness.


Relationship between the effectiveness of lithium therapy and family history.


The current status of systematic densitization.


Freud and the Americans.

Freud and the Americans.


Some aspects of the history of Swiss psychiatry.


Dean McAllister Lierle, MD. In appreciation.


Directory of otolaryngological societies.


Anencephaly: a review.

Anencephaly: a review.


Endotoxin sensitivity of inbred mouse strains.


Methionine biosynthesis and S-adenosylmethionine degradation during an induced morphogenesis of Candida albicans.


Indications and contraindications for vaginal hysterectomy.


Michael Somogyi, Ph. D. (1833-1971).

Michael Somogyi, Ph. D. (1833-1971).


Nutritional therapy in acute myocardial infarction.


Characterization of splenic lymphoid cells in fetal and newborn mice. In order to clarify the cellular events that precede the onset of immunological competence in the mouse, we have characterized and quantitated the lymphoid cells of the spleen as a function of age. Our results show that T cells and B cells both appeared in the spleens of Swiss-L mice as early as the 15th-16th day of gestation. Antigen-binding cells specific for each of three different antigens were also first detected during this same 24 h interval. The B cells and three varieties of antigen-binding cells increased in number rapidly and in parallel until about 1 wk after birth. The T cells, which were more numerous than B cells at first, increased in number somewhat more slowly. Coincident with the onset of response to antigen, there was a further increase in B cell numbers and a decrease in the T cell to B cell ratio. The capacity to respond to antigen by cellular proliferation and synthesis of antibody did not arise until about 2 wk after birth although there were no quantitative changes in the total numbers of T cells, B cells, and antigen-binding cells between 1 and 2 wk of age. Some qualitative change, such as the functional maturation of an antigen-reactive cell, may be required during this interval for the onset of this immunological response. Although the numbers of antigen-binding cells present in fetuses and young animals were smaller than in adults, we have as yet been unable to detect any restriction in the variety of specificities that can be expressed in fetuses, either in the kinds of antigens bound or in the range of avidities with which a single antigen is bound.


Role of the clotting system in cell-mediated hypersensitivity. I. Fibrin deposition in delayed skin reactions in man. The expression of delayed-type hypersensitivity in animals has been inhibited by a variety of anticoagulants, but direct evidence for activation of clotting in the evolution of these reactions has been lacking. Using the fluorescent antibody technique we here demonstrate that fibrin deposition is a prominent and consistent feature of both allergic contact dermatitis and classic delayed hypersensitivity skin reactions in man. Fib was detected in 55 of 58 delayed reactions studied at the peak of their intensity. The characteristic distribution of Fib-principally in the intervascular portions of the reticular dermis with sparing of vessels and their associated cuffs of mononuclear cells-is unusual and quite different from that described in antibody-mediated lesions in animals or man. Fib was found in vessel walls in only 2 of 94 biopsies studied. With a single exception, deposition of immunoglobulins and complement was not observed. The pathogensis and significance of Fib deposition in these reactions are not yet clear. Fib is ultimately derived from circulating fibrinogen, and its accumulation provides additional evidence for locally increased vascular permeability in delayed hypersensitivity. Polymerization of extravascular fibrinogen could be triggered nonspecifically by dermal elements (e.g., collagen) or by a product of sensitized lymphocytes. The appearance of Fib early in the development of these reactions (4-8 h after epicutaneous test with DNCB) and inhibition studies with anticoagulants together suggest that clotting may have a role in their pathogenesis, possibly by the release of bioactive peptides from fibrinogen/fibrin or by contributing to the induration characteristic of delayed hypersensitivity.


A comparative study of doxepin versus trifluoperazine in anxiety neurosis.


Salmonella meningitis in infants.

Salmonella meningitis in infants.


In vitro studies of the human mammary gland: effect of hormones on proliferation in primary cell cultures.


Streptokinase: a review of its clinical pharmacology, mechanism of action and therapeutic uses.


Molecular architecture of the chloroplast membranes of Chlamydomonas reinhardi as revealed by high resolution electron microscopy.


Electron microscopy of polyheads of bacteriophage T4 prepared by freeze-etching.


Efficacy and specificity of chenodeoxycholic acid therapy for dissolving gallstones.


By the London post. Care of the dying--kidneys for transplant--topics of common concern.


The presentation and diagnosis of 100 patients with primary biliary cirrhosis.


"Tight girdle" or Sömmerring's syndrome.


Perceptual rigidity in paranoid schizophrenia: use of projective animal drawings.


Sjogren's syndrome. 2. Clinical associations and immunological phenomena.


A comparative study of four tests of pancreatic function in the diagnosis of pancreatic disease.


The pulsed dye laser as a light source for the fluorescent antibody technique.


The women's draft. An analysis of the controversy over the nurses' Selective Service Bill of 1945.


Immunological characterization of lymphocytes in synovial fluid from patients with rheumatoid arthritis.


Membrane-bound immunoglobulin (Ig) and in vitro production of Ig by lymphoid cells from patients with primary immunodeficiencies.


The effect of capping by anti-immunoglobulin antibody on the expression of cell surface immunoglobulin and on lymphocyte activation.


Antibodies to cell organoids in the sera of nasopharyngeal carcinoma patients and of systemic lupus erythematosus patients.


Occupational class and symptoms of depression.


Effect of antigenic stimulation on the enzymatic activity of mouse peritoneal and spleen cells.


Comparative study of the anorexigenic activity of 5-(3,4-dichlorophenoxymethyl)-2-amino-2-oxazoline HCl and d-amphetamine in different species.


Kenneth Patrick DuBois (August 9, 1917--January 24, 1973).


Decreased drug binding by serum albumin during renal failure.


Earl H. Dearborn (June 10, 1915--March 1, 1973).


Dr. David Milford Hume.

Dr. David Milford Hume.


Dr. Thomson.

Dr. Thomson.


Escherichia coli antigens as dietary additives for oral immunisation of pigs: trials with pig creep feeds.


Surgical treatment of congenital esotropia.


Viral infection of the aorta of man associated with early atherosclerotic changes.


Immunopathologic studies in glomerular diseases with membranous lesions.


Computer-assisted identification of bacteria.


Preparation of intestine and other elongated specimens for histologic and immunofluorescent studies.


Lorazepam (WY 4036) as a pre-operative medication.


Respiratory tract specimens from intensive care patients. Bacterial flora and cytological content.


Ferdinand Edelbert Junker.

Ferdinand Edelbert Junker.


Anaesthetic literature.

Anaesthetic literature.


Unconsidered trifles, no. XXI.

Unconsidered trifles, no. XXI.


Inhibition of deoxythymidine kinase activity by the virostatic 5-ethyl-2'-deoxyuridine.


New approach to the evaluation of antibacterial activity of aminosidine.


Single-dose treatment of epidemic typhus with doxycyline.


Medical uses of oral zinc.

Medical uses of oral zinc.


LH release following steroid administration in ovariectomized rats.


Mineral nutrition and production disease in dairy cows.


Time interval between injection of estradiol benzoate and LH release in the rat and effect of actinomycin D or cycloheximide.


Effect of sex steroids on pituitary responses to LH-and FSH-releasing hormone in vitro.


Some "in vitro" experimental methods in allergic contact dermatitis.


Amino acid conversion into 5-hydroxytryptamine in pancreatic beta-cells.


Estrogen-dependent 24-hour periodicity in pituitary LH release in the female hamster.


Growth hormone responses to hypoglycaemia and arginine tests in hyperthyroid and hypothyroid patients.


Too many foods in pursuit of too few preservatives.


Cutaneous reactions to chromium.

Cutaneous reactions to chromium.


Beta adrenergic stimulant aerosols in bronchial asthma: an assessment.


Renal preservation with a modified intracellular hypothermic solution.


Early evaluation of renal function after prolonged hypothermic preservation.


Lymphocyte transformation in renal lymphatics during canine allograft rejection.


Sir Henry Morris, Bart. (1844-1926).

Sir Henry Morris, Bart. (1844-1926).


Successful enhancement of canine renal allografts.


Repression of enzyme synthesis of the pyrimidine pathway in Salmonella typhimurium. It has been reported by other workers that a uridine and probably also a cytidine nucleotide are required for maximal repression of aspartate transcarbamylase encoded by the gene pyrB in Salmonella typhimurium. We have identified the repressing metabolites for three more biosynthetic enzymes, namely, dihydroorotate dehydrogenase (encoded by pyrD), orotidine-5'-monophosphate pyrophosphorylase (encoded by pyrE), and orotidine-5'-monophosphate decarboxylase (encoded by pyrF), as well as examining the repression profiles of aspartate transcarbamylase in more detail. Using a specially constructed strain of S. typhimurium (JL1055) which lacks the enzymes for the interconversion of cytidine and uridine compounds, thus allowing the independent manipulation of endogenous cytidine and uridine nucleotides, we found that a cytidine compound is the primary effector of repression in all cases except for aspartate transcarbamylase where little repression is observed in excess cytidine. For aspartate transcarbamylase, we found that the primary repressing metabolite is a uridine compound.


Effects of regulatory mutations upon methionine biosynthesis in Saccharomyces cerevisiae: loci eth2-eth3-eth10. The effects of mutations occurring at three independent loci, eth2, eth3, and eth10, were studied on the basis of several criteria: level of resistance towards two methionine analogues (ethionine and selenomethionine), pool sizes of free methionine and S-adenosyl methionine (SAM) under different growth conditions, and susceptibility towards methionine-mediated repression and SAM-mediated repression of some enzymes involved in methionine biosynthesis (met group I enzymes). It was shown that: (i) the level of resistance towards both methionine analogues roughly correlates with the amount of methionine accumulated in the pool; (ii) the repressibility of met group I enzymes by exogenous methionine is either abolished or greatly lowered, depending upon the mutation studied; (iii) the repressibility of the same enzymes by exogenous SAM remains, in at least three mutants studied, close to that observed in a wild-type strain; (iv) the accumulation of SAM does not occur in the most extreme mutants either from endogenously overproduced or from exogenously supplied methionine: (v) the two methionine-activating enzymes, methionyl-transfer ribonucleic acid (tRNA) synthetase and methionine adenosyl transferase, do not seem modified in any of the mutants presented here; and (vi) the amount of tRNA(met) and its level of charging are alike in all strains. Thus, the three recessive mutations presented here affect methionine-mediated repression, both at the level of overall methionine biosynthesis which results in its accumulation in the pool, and at the level of the synthesis of met group I enzymes. The implications of these findings are discussed.


Derepression of uridine diphosphate-glucose pyrophosphorylase (galU) in capR(lon), capS, and capT mutants and studies on the galU repressor. Mutation of the capR(lon), capS, or capT genes in Escherichia coli K-12 causes overproduction of capsular polysaccharide leading to a mucoid phenotype. Several of the enzymes involved in capsular polysaccharide synthesis are derepressed in cap mutants. Previously it was shown that uridine diphosphate-glucose (UDPG) pyrophosphorylase, an enzyme involved in the synthesis of three of the nucleotide sugar precursors of the capsule, is derepressed in capR mutants. The control of galU, the gene which codes for UDPG pyrophosphorylase, is described in this study. In addition, it has been found that the enzyme is also derepressed in capS and capT mutants. The effect of galU gene dosage in cap mutants and the wild-type strain (all lysogenic for phi80) was studied by infecting them with the purified transducing phage phi80dgalU. The level of UDPG pyrophosphorylase increased in proportion to the number of galU copies added. The rate of enzyme synthesis in the mutants was about sixfold higher than in the wild type per galU gene added for multiplicities of infection from one to twenty. Thus, all the galU copies added to the wild-type lysogen were repressed. We obtain greater than 20 galU copies per cell by infecting the nonlysogenic strain which allows multiplication of phi80dgalU. With some number of galU copies greater than 20, the rate of UDPG pyrophosphorylase synthesis in the wild type approaches the mutant rate of synthesis. The results suggest that there may indeed be a galU repressor pool in the cell which can be completely titrated. This pool must be composed of more than 20 galU repressor molecules. Since the capR, capS, and capT gene products or combinations thereof are known to control other widely separated operons of the cell besides the galU gene, it is postulated that the galU repressor may be capable of binding other operators. This would account for the relatively large pool of galU repressors per cell.


Lysis of Saccharomyces cerevisiae with 2-deoxy-2-fluoro-D-glucose, an inhibitor of the cell wall glucan synthesis. The effect of a synthetic glucose analogue, 2-deoxy-2-fluoro-d-glucose (FG) on growth and glucose metabolism of Saccharomyces cerevisiae was studied. The addition of FG (0.005-0.05%) to a 2% glucose medium resulted in reduction of the initial growth rate and, after several hours, in a complete cessation of the culture growth. These two events were due to extensive lysis of the population which continued long after the period when no more growth was recorded. Electron microscope examination of lysed cells showed that the lysis was a consequence of a dissolution of the cell walls. FG inhibited to a similar extent the initial growth rate and the incorporation of radioactivity from labeled glucose into growing population. The inhibition of radioactivity incorporation from glucose by growing protoplasts was much less. The yeast was found to be extremely FG sensitive whenever the synthesis of new cell wall material was involved. All observations imply that FG interferes mainly with the cell wall formation of S. cerevisiae. A comparison of the FG effects on metabolic activity of protoplasts, simultaneous secretion of mannan-proteins into the growth medium, and the formation of glucan fibrils on the surface of protoplasts demonstrated that the cell wall glucan synthesis is the most FG-sensitive process and evidently the growth-limiting factor in intact cells. FG-resistant cells were selected during growth experiments. They exhibited an altered mode of cell division when grown in the presence of FG.


Regulation of tyrosine and phenylalanine biosynthesis in Escherichia coli K-12: properties of the tyrR gene product. A spontaneous amber tyrR mutant has been isolated in which constitutive synthesis of 3-deoxy-d-arabinoheptulosonic acid 7-phosphate (DAHP) synthetase (tyr) and DAHP synthetase (phe) is suppressible by supC(-), supD(-), supF(-) and supU(-). This finding suggests the tyrR gene product is a protein. Derepression of DAHP synthetase (phe) in this and in seven other spontaneous tyrR mutants and in four Mu-1-induced tyrR mutants provides further evidence for the involvement of the tyrR gene product in phenylalanine biosynthesis. Evidence that the tyrR product is a component of repressor, rather than an enzyme involved in its synthesis or modification, comes from a study of a temperature-sensitive tyrR mutant. This mutant is of the thermolabile type, since derepression occurs rapidly and in the presence and absence of growth.


Tyrosine and phenylalanine biosynthesis in Escherichia coli K-12: complementation between different tyrR alleles. Dominance tests in diploids have confirmed that the product of the tyrR gene is involved in a negative control system affecting the synthesis of both 3-deoxy-d-arabinoheptulosonic acid 7-phosphate (DAHP) synthetase (tyr) and DAHP synthetase (phe). Some tyrR mutants are derepressed for the synthesis of both DAHP synthetase (tyr) and (phe), whereas others are derepressed for the synthesis of DAHP synthetase (tyr) but overrepressed for the synthesis of DAHP synthetase (phe). Complementation tests between these alleles confirm that they are in the same cistron. The allele causing overrepression of enzyme synthesis is dominant over both the wild type and the derepressing allele in diploids.


Membrane attachment of R-factor deoxyribonucleic acid in compatible and incompatible cell pairs following conjugation. Superinfection of an Escherichia coli R(+) strain with a closely related R-factor was associated with the accumulation of replicative intermediates of the superinfecting deoxyribonucleic acid. This deoxyribonucleic acid remains associated with a rapidly sedimenting cell component.


Nucleoid condensation and cell division in Escherichia coli MX74T2 ts52 after inhibition of protein synthesis. The reorganization of the bacterial nucleoid of an Escherichia coli mutant, MX74T2 ts52, was studied by electron microscopy after protein synthesis inhibition by using whole mounts of cell ghosts, ultrathin-sectioning, and freeze-etching. The bacterial nucleoid showed two morphological changes after chloramphenicol addition: deoxyribonucleic acid (DNA) localization and DNA condensation. DNA localization was observed 10 min after chloramphenicol addition; the DNA appeared as a compact, solid mass. DNA condensation was observed at 25 min; the nucleoid appeared as a cytoplasm-filled sphere, often opened at one end. Ribosomes were observed in the center. Giant nucleoids present in some mutant filaments showed fused, spherical nucleoids arranged linearly, suggesting that the tertiary structure of the nucleoid reflects the number of replicated genomes. Inhibitors which directly or indirectly blocked protein synthesis and caused DNA condensation were chloramphenicol, puromycin, amino acid starvation, rifampicin, or carbonyl cyanide m-chlorophenyl hydrazone. All inhibitors that caused cell division in the mutant also caused condensation, although some inhibitors caused condensation without cell division. Nucleoid condensation appears to be related to chromosome structure rather than to DNA segregation upon cell division.


Genetics and physiology of a tolE mutant of Escherichia coli K-12 and phenotypic suppression of its phenotype by galactose. The tolE mutation causes tolerance to colicins E2 and E3 as well as other effects on the phenotype of Escherichia coli K-12. The lipopolysaccharide of the mutant shows a reduction in the content of galactose, glucose, and rhamnose. The phenotype of the mutant, including the composition of the lipopolysaccharide, is suppressed by galactose. The map position is shown by the gene order trp-purB-tolE-tolD-galKETO.


Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. The sensitivity of the outer and cytoplasmic membranes of Escherichia coli to detergent was examined by isopycnic sucrose density gradient centrifugation. Sodium lauryl sarcosinate (Sarkosyl) was found to disrupt the cytoplasmic membrane selectively under conditions in which Triton X-100 and dodecyl sodium sulfate solubilized all membrane protein. These results were verified by gel electrophoresis; membrane proteins solubilized by Sarkosyl were identical to those of the cytoplasmic membrane. The presence of Mg(2+) during treatment with Sarkosyl was found to afford partial protection of the cytoplasmic membrane from dissolution.


Accumulation of methylglyoxal in a mutant of Escherichia coli constitutive for gluconate catabolism. A culture of a mutant of Escherichia coli, derepressed for gluconate catabolism, is killed by the addition of gluconate to the culture. The product responsible for this bactericidal effect was identified as methylglyoxal. Two types of mutants resistant to gluconate were isolated. One of them showed increased activity of glyoxalase I.


Synthesis of unmethylated deoxyribonucleic acid in a dnaB temperature-sensitive mutant of Escherichia coli starved for methionine. Strain CRT 266, a polyauxotrophic dnaB temperature-sensitive mutant of Escherichia coli K, was investigated for residual deoxyribonucleic acid (DNA) synthesis when returned to the permissive temperature in the absence of protein synthesis. In the presence of methionine, a delayed extra-initiation occurs as well as an erratic long-lasting synthesis. In the absence of methionine, there is no evidence for extra-initiation, whereas the long-lasting synthesis is only slightly depressed. A direct role of methionine independent of protein synthesis in the extra-initiation process is postulated. The largest residual syntheses, with or without methionine, are obtained when (i) bacteria are first grown in a rich medium, (ii) bacteria are shifted to the nonpermissive temperature for 2 h in the same medium, and (iii) bacteria are then starved for aminoacid for 20 h at the permissive temperature. Under these conditions, DNA extracted from methionine-starved cells appears to be a mixture of half-methylated and unmethylated products. The possibility of the occurence of a few methyl groups on the so-called unmethylated DNA is discussed.


Coupling between chromosome completion and cell division in Escherichia coli. The relationship between termination of chromosome replication and cell division was investigated in Escherichia coli B/r. Synchronous cultures of E. coli B/r growing in glucose minimal medium or subjected to a nutritional shift-up were exposed to chloramphenicol, rifampin, mitomycin C, or nalidixic acid, and the ability of cells to divide in the presence of the inhibitors was measured. It was found that cell division became resistant to inhibitors of ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) synthesis at approximately the same stage in the division cycle in all situations investigated. When the synchronous glucose-grown cultures were temporarily exposed to chloramphenicol early in the division cycle and then exposed to mitomycin C or nalidixic acid immediately after removal of chloramphenicol, the cells did not divide. In contrast, when DNA synthesis was inhibited by thymine starvation immediately after temporary exposure to chloramphenicol, cells divided. The results suggest that DNA chain elongation is completed in some cells in the absence of protein synthesis, but that additional steps involving specific RNA or protein synthesis, or both, may be required for processing the chromosomal structures to the form which is necessary for division. This processing, which normally occurs concurrent with DNA synthesis and is prevented by inhibitors of DNA synthesis, may trigger division. Alternatively, in the absence of protein synthesis, all aspects of chromosome formation may be completed, but final transcriptional events which are essential for division cannot take place until the complete synthesis of a critical amount of specific proteins.


Cytoplasmic "petite" induction in recombination-deficient mutants of Saccharomyces cerevisiae. As compared to the original wild type, the induction of the cytoplasmic "petite" mutation by ultraviolet light and by the intercalating dye, ethidium bromide, is reduced in two mutants (rec4 and rec5) of Saccharomyces cerevisiae. These mutants are blocked in X rays or ultraviolet light-induced intragenic recombination. It then appears that the products of nuclear genes necessary for the completion of nuclear intragenic recombination events are also involved in steps of the metabolic chain which leads to the mitochondrial mutation, rho(-).


Three kinds of controls affecting the expression of the glp regulon in Escherichia coli. Three kinds of control mechanisms govern the expression of the members of the glp regulon for glycerol and sn-glycerol 3-phosphate (G3P) catabolism in Escherichia coli K-12: specific repression by the product of the glpR gene; catabolite repression; and respiratory repression (the effect exerted by exogenous hydrogen acceptors). The operons of the glp system show different patterns of response to each control. By growing in parallel a mutant strain with temperature-sensitive repressor (glpR(ts)) and an isogenic control with a deletion in the regulator gene at progressively higher temperatures, it was possible to show that the synthesis of aerobic G3P dehydrogenase (glpD product) is far more sensitive to specific repression than that of either glycerol kinase (glpK product) or G3P transport (glpT product). Conversely, in the strain with a deletion in the regulator gene, the syntheses of glycerol kinase and G3P transport are more sensitive to catabolite repression than that of the aerobic G3P dehydrogenase. The levels of the two flavoprotein G3P dehydrogenases vary in opposite directions in response to changes of exogenous hydrogen acceptors. For example, the ratio of the aerobic enzyme to the anaerobic enzyme (specified by glpA) is high when molecular oxygen or nitrate serves as the hydrogen acceptor and low when fumarate plays this role. This trend is not influenced by the addition of cyclic adenosine 3',5'-monophosphate to the growth medium. Thus, respiratory repression most likely involves a third mechanism of control, independent of specific or catabolite repression.


Chemotaxis toward sugars in Escherichia coli. Using a quantitative assay for measuring chemotaxis, we tested a variety of sugars and sugar derivatives for their ability to attract Escherichia coli bacteria. The most effective attractants, i.e., those that have thresholds near 10(-5) M or below, are N-acetyl-d-glucosamine, 6-deoxy-d-glucose, d-fructose, d-fucose, 1-d-glycerol-beta-d-galactoside, galactitol, d-galactose, d-glucosamine, d-glucose, alpha-d-glucose-1-phosphate, lactose, maltose, d-mannitol, d-mannose, methyl-beta-d-galactoside, methyl-beta-d-glucoside, d-ribose, d-sorbitol, and trehalose. Lactose, and probably d-glucose-1-phosphate, are attractive only after conversion to the free monosaccharide, while the other attractants do not require breakdown for taxis. Nine different chemoreceptors are involved in detecting these various attractants. They are called the N-acetyl-glucosamine, fructose, galactose, glucose, maltose, mannitol, ribose, sorbitol, and trehalose chemoreceptors; the specificity of each was studied. The chemoreceptors, with the exception of the one for d-glucose, are inducible. The galactose-binding protein serves as the recognition component of the galactose chemoreceptor. E. coli also has osmotically shockable binding activities for maltose and d-ribose, and these appear to serve as the recognition components for the corresponding chemoreceptors.


Inhibitors of ribonucleic acid synthesis in Saccharomyces cerevisiae: decay rate of messenger ribonucleic acid. Daunomycin and ethidium bromide, two deoxyribonucleic acid-intercalating drugs, inhibit ribonucleic acid (RNA) and protein synthesis in Saccharomyces cerevisiae. Both agents rapidly curtail uptake of radioactive adenine, whereas the kinetics of radioactive leucine uptake after drug addition are consistent with translation of a pool of exponentially decaying messenger RNA. Messenger RNA half-life determinations from these experiments gave identical results over a range of drug concentrations; this value is 21 +/- 4 min at 30 C. In a temperature-sensitive mutant in which RNA synthesis is curtailed at the nonpermissive temperature, a similar half-life for messenger RNA decay is found both in the absence and in the presence of either drug. This indicates that at the concentrations used in this study, neither daunomycin nor ethidium bromide has an appreciable direct effect on translation and do not increase the lability of messenger RNA.


Isolation of the nonviable cells produced during normal growth of recombination-deficient strains of Escherichia coli K-12. During normal growth, cultures of recombination-deficient (Rec(-)) strains contain a population of cells that do not form colonies. Such cells are not present in a culture of an isogenic Rec(+) strain. We present a procedure for isolating and studying this defective population of cells. Exposure of a growing Rec(-) or Rec(+) culture to low levels of penicillin causes the dividing cells to elongate. The size of the nonviable cells present in the Rec(-) cultures is unaffected. The nonviable cells are then separated from the elongated cells by velocity sedimentation. This isolation technique provides a convenient way of analyzing the composition, biosynthetic capacity, and enzymatic function of the nonviable cells before isolation. In this paper we present data showing that before fractionation the nonviable cells in the Rec(-) culture are defective in their ability to synthesize beta-galactosidase, whereas the Rec(-) viable cells behave like the Rec(+) cells in this regard. This observation confirms the existence of at least two classes of cells in liquid cultures of Rec(-) strains grown under normal conditions. That class of cells which is unable to synthesize beta-galactosidase is the same class that cannot form colonies when plated on solid medium.


Three additional genes required for deoxyribonucleic acid synthesis in Saccharomyces cerevisiae. Deoxyribonucleic acid (DNA) synthesis was examined in asynchronous and synchronous cultures of a number of cdc (cell division cycle) temperature-sensitive mutant strains. The kinetics of DNA synthesis after a shift to the restrictive temperature was compared with that obtained after inhibition of protein synthesis at the permissive temperature, a condition that specifically blocks the initiation of new rounds of DNA replication, but does not block those in progress. Mutations in three genes (cdc 4, 7, and 28) appear to block a precondition for DNA synthesis since cells carrying these lesions cannot start new rounds of DNA replication after a shift from permissive to restrictive temperature, but can finish rounds that were in progress. These three genes are classified as having roles in the "initiation" of DNA synthesis. Mutations in two genes (cdc 8 and 21) block DNA synthesis, itself, since cells harboring these lesions that had started DNA synthesis at the permissive temperature arrest synthesis abruptly upon a shift to the restrictive temperature. Mutations in 13 other cdc genes do not impair DNA synthesis in the first cell cycle at the restrictive temperature.


Amino acid transport in a polyaromatic amino acid auxotroph of Saccharomyces cerevisiae. The initiation of growth of a polyaromatic auxotrophic mutant of Saccharomyces cerevisiae was inhibited by several amino acids, whereas growth of the parent prototroph was unaffected. A comparative investigation of amino acid transport in the two strains employing (14)C-labeled amino acids revealed that the transport of amino acids in S. cerevisiae was mediated by a general transport system responsible for the uptake of all neutral as well as basic amino acids. Both auxotrophic and prototrophic strains exhibited stereospecificity for l-amino acids and a K(m) ranging from 1.5 x 10(-5) to 5.0 x 10(-5) M. Optimal transport activity occurred at pH 5.7. Cycloheximide had no effect on amino acid uptake, indicating that protein synthesis was not a direct requirement for amino acid transport. Regulation of amino acid transport was subject to the concentration of amino acids in the free amino acid pool. Amino acid inhibition of the uptake of the aromatic amino acids by the aromatic auxotroph did not correlate directly with the effect of amino acids on the initiation of growth of the auxotroph but provides a partial explanation of this effect.


Superoxide dismutases of Escherichia coli: intracellular localization and functions. Escherichia coli B contains two superoxide dismutases which differ with respect to their localization within the cell, the nature of their prosthetic metals, their responses to changes in (p)O(2), and their functions. One of these enzymes, which was liberated from the cells by osmotic shock and which was therefore presumed to be localized in the periplasmic space, is an iron-containing superoxide dismutase. The amount of this iron enzyme did not vary in response to changes in (p)O(2) during growth. In contrast, the other superoxide dismutase was not solubilized by osmotic shock, was a mangano-protein, and was found in greater amounts in cells which had been grown at high (p)O(2). E. coli, which had low levels of the iron-enzyme and high levels of the mangano-enzyme, as a consequence of growth in iron-deficient aerated medium, was killed by exposure to an exogenous flux of O(2) (-) which was generated either photochemically or enzymatically. The addition of bovine superoxide dismutase to the suspending medium protected these cells against this stress. On the other hand, E. coli, which had high levels of the iron-enzyme and low levels of the mangano-enzyme, as a consequence of growth in iron-rich anaerobic medium, was resistant to exogeneous O(2) (-). On the basis of these and of previously reported results (4a, Yost, F. J. and I. Fridovich, J. Biol. Chem., 1973, in press), it appears that the iron superoxide dismutase, of the periplasmic space, serves as a defense against exogenous O(2) (-), whereas the mangano-superoxide dismutase, in the matrix of these cells, serves to counter the toxicity of endogenous O(2) (-).


Whole-blood culture technique for functional studies of lymphocyte reactivity to mitogens, antigens, and homologous lymphocytes.


A case of Osler's disease.

A case of Osler's disease.


Comparison of hycanthone ("etrenol"), some hycanthone analogs, myxin and 4-nitroquinoline-1-oxide as frameshift mutagens.


Autoradiographic study of the rejection phenomenon in tissue culture.


The functional state of kidneys perfused at 37 degrees C with a bloodless fluid.


Preliminary report. Hypothermic renal storage. I. Adverse effect of papaverine.


Medicine and change.

Medicine and change.


Cardiovascular actions of vasopressin.

Cardiovascular actions of vasopressin.


The curtain falls.

The curtain falls.


Comparative pathogenesis of rabies and rabies-like viruses: infection of the central nervous system and centrifugal spread of virus to peripheral tissues.


Trimethoprim-sulphamethoxazole in the treatment of urinary infection.


Co-trimoxazole in urinary tract infection.


Use of combinations of trimethoprim and sulphamethoxazole in the treatment of chest infections.


Treatment of nocardial infection with trimethoprim-sulphamethoxazole.


Biochemical background of trimethoprim-sulphamethoxazole.


Malaria in the Australian army in South Vietnam: successful use of a proguanil-dapsone combination for chemoprophylaxis of chloroquine-resistant falciparum malaria.


Accepting site for aminoacylation of tRNAphe from yeast.


Verification of misacylated tRNAphe is apparently carried out only by phenylalanyl-tRNA synthetase.


Gut and islet hormones in diabetes.

Gut and islet hormones in diabetes.


Screening for cervical cancer.

Screening for cervical cancer.


Nifuratel in the treatment of vaginitis in general practice.


A comparative trial of different regimens of fenfluramine and phentermine in obesity.


Harrogate, the Yorkshire Spaw.

Harrogate, the Yorkshire Spaw.


Adie's syndrome. William John Adie (1886-1935).


Sexual stimulation and displaced aggression.


Change in stimulus conditions and the orienting response.


Roentgen diagnosis of aortic disease.

Roentgen diagnosis of aortic disease.


Dissecting aneurysm of the aorta.

Dissecting aneurysm of the aorta.


Dr. Ephraim Luzzatto, physician and poet 1729-1792.


Arteriosclerotic aneurysms of the abdominal aorta: a review.


A vitreous suction cutter for aphakic keratoplasty.


Bernard C. Gettes, M.D. 1912-1973.

Bernard C. Gettes, M.D. 1912-1973.


Detection of Histoplasma capsulatum by fluorescent antibody techniques in experimental ocular histoplasmosis.


The effects of tidal volume and end-expiratory pressure on pulmonary gas exchange during anesthesia.


Mallophaga indica. VII. Historical résumé and bibliography of Mallophaga indica.


Clinical considerations for the anesthesiologist whose patient is on anticoagulant therapy.


Esophageal anastomosis.

Esophageal anastomosis.


The airborne component of wound contamination and infection.


Immunological aspects of cation transport in sheep red cells.


Fluorescent probes in membrane studies.

Fluorescent probes in membrane studies.


Lipids of the cell plasma membrane.

Lipids of the cell plasma membrane.


Recent studies using nuclear magnetic resonance spectroscopy of lipids and biological membranes.


Properties of water in red cell and synthetic membranes.


Mechanisms of anion permeation. A review of available data, principally on muscle cells, with the fixed charge concept in mind.


Conductance mechanisms in excitable cells.


Chemical modification of proteins involved in the permeability of the erythrocyte membrane to ions.


Membrane noise and ion transport in the node of Ranvier.


The Aharon Katchalsky memorial lecture. "Life, flow and deformation".


The use of mechanical tests in determining the structure of connective tissues.


Rheological properties of chromosomal nucleoproteins.


Poiseuille award ceremony for A. L. Copley.


On biorheology.

On biorheology.


Influence of recipient's immunization on isograft infiltration.


The synergistic effect of antigen and hydrocortisone on the survival time of skin allografts in inbred rats.


Pituitary response to gonadotropin-releasing hormone (Gn-RH) stimulation in normal subjects.


The significance of urinary beta-aminoisobutyric acid in cancer patients.


The effect of some nucleic acid-binding metabolic inhibitors on split-dose sparing in u.v.-and electron-irradiated diploid yeast.


DNA repair and degradation in x-irradiated E. coli B-r cells after treatment with ultra-violet light and N-ethylameimide.


Production of functional T cells after treatment of bone marrow with thymic factor.


Effect of locally administered endotoxin on regenerating appendix structure and responses of appendix cells to mitogens.


Anti-alloantibodies: a de novo product.

Anti-alloantibodies: a de novo product.


Transient appearance of cells secreting antibody of different specificities after immunization of mice with trinitrophenylated erythrocytes.


Studies on early events in the differentiation of antibody-producing cells. I. Patterns of deoxyribonucleic acid replication in antigen-stimulated mouse spleen cells.


Regulation of the immune response. IV. Antibody-mediated suppression of the immune response to haptens and heterologous erythrocyte antigens in vitro.


Immunoglobulin biosynthesis by normal and leukemic human peripheral blood lymphocytes.


T cell surface markers on lymphoblasts from acute lymphocytic leukemia.


The in vitro response of chicken B cell populations to lipopolysaccharide.


Use of normal mouse serum to detect indirect plaque-forming cells.


L-colony production by different strains of Staphylococcus aureus in the absence of antibiotics.


Nonspecificity of the limulus amebocyte lysate test: positive reactions with polynucleotides and proteins.


Replication of bacteriophage lambda DNA dependent on the function of host and viral genes. I. Interaction of red, gam and rec.


Location of the first step transfer fragment and single-strand interruptions in T5stO bacteriophage DNA.


Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors.


Structural dynamics of bacterial ribosomes. I. Characterization of vacant couples and their relation to complexed ribosomes.


Lac repressor-operator interaction. 8. Lactose is an anti-inducer of the lac operon.


Bacteriophage T4 tail assembly: structural proteins and their genetic identification.


Bacteriophage T4 tail assembly: proteins of the sheath, core and baseplate.


Cyclic blockade of initiation sites by streptomycin-damaged ribosomes in Escherichia coli: an explanation for dominance of sensitivity.


Effect of streptomycin on the response of Escherichia coli ribosomes to the dissociation factor.


X-ray crystallographic studies of polymorphic forms of yeast phenylalanine transfer RNA.


Unit cell transormations in yeast phenylalanine transfer RNA crystals.


Growth instability of the alkaline phosphatase repressor.


Genome independent specificity of DNA-directed fMet-dipeptide synthesis.


The new Meharry number.

The new Meharry number.


Meharry Medical College in retrospect.

Meharry Medical College in retrospect.


The practice of dilution methods for the estimate of cardiac output, pulmonary and ventricular blood volumes with radioisotopes.


Bacterial killing by bacteriostatic saline solutions--potential for diagnostic error.


Quantitative scintiangiocardiography: measurement of heart chamber volumes by scintillation camera recorded dilution curves.


General model for the replication of double stranded DNA molecules.


Instruments and radionuclides for myocardial perfusion measurements.


Amino acid acceptor stem of E. coli suppressor tRNA tyr is a site of synthetase recognition.


Radionuclide imaging in the evaluation of cardiac disease (the role of myocardial perfusion imaging and radionuclide angiography).


Does Escherichia coli possess a DNA excision repair system for gamma-ray damage?


Theory of current techniques of measuring cerebral blood flow.


Performance and results of myocardial blood flow measurements using radioactive noble gases.


Pathophysiological, diagnostic and methodological problems in the study of myocardial blood flow in ischaemic heart disease.


Relevance of regional perfusion measurements (regional cerebral blood flow equals rCBF).


Medical writings of Moses Maimonides.

Medical writings of Moses Maimonides.


Utility and limitations of myocardial perfusion measurements with radionuclides using a probe detector.


Fungistatic activity, uptake and incorporation of 5-fluorocytosine in Candida albicans, as influenced by pyrimidines and purines. II. Studies on distribution and incorporation.


Nonhistone chromosomal proteins in control of cell division.


Immunosuppressive effects of specific classes of agents with special reference to organ transplantation. Immunosuppression by thiopurines.


Immunosuppression by alkylating agents--tolerance induction.


Infectious complications in renal transplant recipients.


Infectious complications in cardiac transplant patients.


Diagnosis and treatment of pneumocystosis and toxoplasmosis in the immunosuppressed host.


Hyperacute rejection of orthotopic cardiac allografts in dogs following solubilized antigen pretreatment.


Redistribution and fate of Ig complexes on surface of B lymphocytes: functional implications and mechanisms.


Heterotopic tooth transplantation. A case against the tooth socket being a semiprivileged site.


The effect of lung allografting on reactive blastogenesis by dog leukocytes.


HK239: a P2 related temperate phage which excludes rII mutants of T4.


A new species of small covalently closed f1 DNA in Escherichia coli infected with an amber mutant of bacteriophage f1.


Electron microscope heteroduplex mapping of P2 Hy dis bacteriophage DNA.


An analysis of the processes of infection and induction of E. coli mutant hfl-1 by bacteriophage lambda.


Abortive infection of Escherichia coli with the bacteriophage f1: cytoplasmic membrane proteins and the f1 DNA-gene 5 protein complex.


Regulation of synthesis of bacteriophage T7 lysozyme mRNA.


The genetics and physiology of coliphage N4.


Postirradiation fate of bacteriophage lambda DNA in the host cell.


Location of the DNA-adenine methylase gene on the genetic map of phage T2.


Orientation of prophage Mu.

Orientation of prophage Mu.


Abortive infection of Escherichia coli with the bacteriophage f1: DNA synthesis associated with the membrane.


Inhibition of growth of strains of Salmonella typhimurium by meso-tartrate.


Benjamin Rush: morality in government.

Benjamin Rush: morality in government.


Experimental urinary infection in rats: pregnancy outcome and its relationship to uterine contractility.


Intellectual impairment in uremic patients.


The urokinase pulmonary embolism trial.

The urokinase pulmonary embolism trial.


Peritoneal adhesions and their relation to abdominal surgery. A postmortem study.


Role of inoculation site as a determinant of infection in soft tissue wounds.


Does central venous pressure accurately reflect hemodynamic and fluid volume patterns in the critical surgical patient?


In memoriam. Linda Hilles, M.D. (1932-1973).


Activities of B. stearothermophilus 50 S ribosomes reconstituted with prokaryotic and eukaryotic 5 S RNA.


In vivo and in vitro transcription of T7 early genes by T7 RNA polymerase.


The binding of Q  initiator fragments to E. coli ribosomes.


Clonorchiasis studied by percutaneous cholangiography, and a therapeutic trial of toluene-2,4-diisothiocyanate. A case report.


Microbial flora of the upper small bowel in Crohn's disease.


Howard E. Ticktin. 1927-1973.

Howard E. Ticktin. 1927-1973.


The Journal of Bone and Joint Surgery. The editorial team.


The bicentenary of Abraham Colles, 1773-1843.


Atlanto-axial fracture-dislocations. Failure of posterior C.1 to C.2 fusion.


Colles's classic description of fractures of the lower end of the radius.


Mortality from stroke among U.S. veterans in Georgia and 5 western states. I. Study plan and death rates.


Mortality from stroke among U.S. veterans in Georgia and 5 western states. 3. Hypertension and demographic characteristics.


Achievement, culture and personality: the case of William Caudill. An editorial.


Mortality from stroke among U.S. veterans in Georgia and 5 western states. IV. Clinical observations.


Influence of inhalation of 100 percent oxygen on intrapulmonary shunt in severely traumatized patients.


Treatment of posttraumatic osteomyelitis with bone, soft tissue, and skin defects.


Studies on indicator dilution curves using mathematical modelling and digital computer techniques.


Sir Arthur Elton (1906-1973).

Sir Arthur Elton (1906-1973).


Aspects of Islamic medical jurisprudence.


Value of lymph-node biopsy in the diagnosis of acute acquired toxoplasmosis.


By the London post. Voluntary service--hypochondria, the layman's specialty--Dr. Joseph Garland.


Cytosine arabinoside versus virus or man?


Varieties of significance.

Varieties of significance.


On titanic life-serving spirits.

On titanic life-serving spirits.


Topography of outer membrane growth in E. coli.


Possible involvement of DNA polymerase I in excision of RNA from Col E1 DNA in vivo.


Occurrence of a DNA component in preparations of Escherichia coli ribosomes.


Use of protection of 30S ribosomal proteins by tRNA for functional mapping of the E. coli ribosome.


Effect of Mycobacterium bovis BCG-Praha on the growth of transplanted Daels' sarcoma in guinea-pigs.


Chitosanase, a novel enzyme.

Chitosanase, a novel enzyme.


Immunosuppression with Cytembena: effect on antibody plaque-forming spleen cells and on hemolytic antibodies production.


Editorial: to Perc Phillips--on retirement.


A tribute to Percy T. Phillips.

A tribute to Percy T. Phillips.


Collected credos--Percy T. Phillips.

Collected credos--Percy T. Phillips.


Schedule-induced polydipsia: blockade by intrahypothalamic atropine.


Sex differences in body weight following gonadectomy and goldthioglucose injections in mice.


Differentiation of electrical rhythms and functional specificity of the hippocampus of the rat.


Cardiovascular responses of rabbits to ESB: effects of anesthetization, stimulus frequency and pulse-train duration.


Passive avoidance deficit with septal lesions: disturbance of response inhibition or response acquisition?


Late effects of early hunger and mother-litter separation on learning and memory in male rats.


Recurrent appendicitis: fact not fallacy.


An experience in ambulatory medicine.

An experience in ambulatory medicine.


Management of adult hip disease.

Management of adult hip disease.


Steatorrhoea and jejunal reticulum cell sarcoma occurring in a patient on long-term colchicine therapy for gout.


Experimental approaches to endometrial and ovarian cancer.


Statistical aspects of a co-operative trial on the treatment of ovarian carcinoma.


Ovarian cancer clinical survey.

Ovarian cancer clinical survey.


The psychiatric sequelae of head injury: a review.


Intestinal immunoglobulins in ulcerative colitis.


Colonoscopy in ulcerative colitis.

Colonoscopy in ulcerative colitis.


Assessment of the relationship between gastric secretory capacity and jejunal bacteriology.


Gastric secretion of pepsin in patients with primary hyperparathyroidism before and after removal of parathyroid adenomas.


Qualitative and quantitative studies of antral and fundic G cells in pernicious anaemia.


Allen O. Whipple surgical society. Introduction.


Town-gown relations and affiliated hospital residency programs.


The gallbladder as a conduit between the liver and intestine.


The treatment of extruded and infected permanent cardiac pulse generators: application of a technique of closed irrigation.


Ultrastructure of fenestrated cells in the sinusoidal wall of rat liver after perfusion fixation.


Anesthetic management of allotransplantation of kidney for the patient with chronic renal failure: clinical study on acid-base, electrolytes and other metabolites.


The early infancy of preventive cardiology.


Walter Reed in Florida.

Walter Reed in Florida.


The chair and venous thrombosis.

The chair and venous thrombosis.


Memorial: Chester Scott Keefer, M.D.

Memorial: Chester Scott Keefer, M.D.


Acquired toxoplasmosis--selected aspects of diagnosis.


Memorial: Colin Munro MacLeod, M.D.

Memorial: Colin Munro MacLeod, M.D.


Memorial: George Cheever Shattuck, M.D. 1879-1972.


Philip Drinker.

Philip Drinker.


History of the Academy's scientific exhibits.


Presence of the human blood group substance P in gram negative bacilli.


Subunit structure of  -acetohydroxy acid isomeroreductase from Salmonella typhimurium.


Prognosis of keratoplasty in phakic and aphakic patients and use of cryopreserved donor tissue.


Ribonucleotide reductase activity during the cell cycle of Saccharomyces cerevisiae.


Purification and properties of thiaminephosphate pyrophosphorylase of Escherichia coli.


Effect of methionine and vitamin B-12 on the activities of methionine biosynthetic enzymes in metJ mutants of Escherichia coli K12.


Corneal transplantation for severe alkali burns.


Selective inhibition of f2 RNA translation by sulfhydryl reagents.


The disulfide bridges of duck egg-white lysozyme II.


Differential regulation of the N-methyl transferases responsible for phosphatidylcholine synthesis in Saccharomyces cerevisiae.


A history of water fluoridation.

A history of water fluoridation.


The effects of partial denture design on the mobility of abutment teeth. An investigation.


Prevention of thromboembolism.

Prevention of thromboembolism.


Reports of extraordinary fecundity in early California.


Sir Ronald Ross and the evolution of malaria research.


Physiologic advantages of grunting, groaning, and pursed-lip breathing: adaptive symptoms related to the development of continuous positive pressure breathing.


Benjamin West's osteomyelitis: a translation.


Robert James Graves on the geography of disease.


Usefulness of the thermometer.

Usefulness of the thermometer.


Foreign medical textbooks (Samuel D. Gross).


Panel report. Diagnosis, staging, and criteria of response to therapy for lung cancer.


Rationale of combination chemotherapy based on preclinical experiments.


Panel report. Interdisciplinary group on clinical trials (biostatistics).


Keynote address on biostatistics and data retrieval.


Frequency of residual and metastatic tumor in patients undergoing curative surgical resection for lung cancer.


Reliability of cytologic diagnosis in cancer of the lung.


Welcome from the Veterans' Administration.


Comparative results of right pneumonectomy and sleeve lobectomy.


The "Spanish" influenza pandemic of 1918 and its impact on the Southern Rhodesian mining industry.


The use of dimethothiazine in the treatment of childhood cerebral palsy.


The cardiofacial syndrome: report of a case with severe facial palsy.


Arresting premature labour: orciprenaline and other drugs.


Lorazepam--another benzodiazepine.

Lorazepam--another benzodiazepine.


Changes in the activity of inorganic pyrophosphatase in a temperature-sensitive DNA-synthesis mutant of Escherichia coli.


Electrophoretic separation of Escherichia coli ribosomal particles on polyacrylamide gels.


The dissociation of Escherichia coli ribosomes.


Primary structure of bovine growth hormone.


Identification of the lipid intermediate in yeast mannan biosynthesis.


Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli.


Replication of colicinogenic factor E1 in Escherichia coli. Properties of newly replicated supercoils.


Antigen binding rosette forming cells in a Friend virus-induced leukemia.


Second-set reaction favouring incorporation of bone allografts.


Evaluation of antilymphocyteserum: comparison of skinallograft survival and rosette inhibition.


Thermo-regulatory responses to hypothalamic heating in dehydrated rabbits.


The effect of unilateral and bilateral lesions in the locus coeruleus on the levels of 3-methoxy-4-hydroxyphenylglycol (MHPG) in neocortex.


Amphibian organ culture.

Amphibian organ culture.


Neuron-neuronal attachments in the parietal cortex of the rat.


Recycling of the initiation factor IF-3 on 30 S ribosomal subunits of E. coli.


The primary structure of rabbit liver tRNA Phe and its comparison with known tRNA Phe sequences.


The occurrence of free L-pyrrolidone carboxylic acid in body fluids and tissues.


Identification of insulin synthesizing polysomes in isolated islets of Langerhans from rat pancreas.


Methylated oligonucleotides derived from bacteriophage fd RF-DNA modified in vitro by E. coli B modification methylase.


Periodic changes in rate of amino acid uptake during yeast cell cycle. Uptake of amino acids is a complex process but in cells growing with ammonia as sole nitrogen source the initial uptake rate of amino acids is a measure of the transport capacity of the uptake system (permease). In synchronous cultures of Saccharomyces cerevisiae amino acids were transported at all stages of the cell cycle. However, for any one amino acid the initial uptake rate was constant for most of the cycle and doubled during a discrete part of the cycle. Thus, for a variety of amino acids the functioning amino acid transport capacity of the membrane doubles once per cycle at a characteristic stage of the cycle. Arginine, valine, and phenylalanine exhibit periodic doubling of uptake rate at different stages of the cell cycle indicating that the transport of these amino acids is mediated by three different systems. Serine, phenylalanine, and leucine exhibit periodic doubling of the uptake rate at the same stage of the cycle. However, it is unlikely that serine and phenylalanine share the same transport system since the uptake of one is not inhibited by the other amino acid. This phenomenon is analogous to the periodic synthesis of soluble enzymes observed in S. cerevisiae.


Trichodermin, a possible inhibitor of the termination process of protein synthesis.


Three approaches to the study of parent-child interaction: ethological, interview and experimental.


Bibliography of column chromatography 1967-1970 and survey of applications.


A crystallographic study of alkaline phosphatase at 7-7 Angstrom resolution.


Ribosomal proteins. 43. In vivo assembly of Escherichia coli ribosomal proteins.


Autoradiographic evidence for bidirectional DNA replication in Escherichia coli.


Studies on superinfection immunity among transmissible plasmids in Escherichia coli.


Physical study of prophage excision and curing of lambda prophage from lysogenic Escherichia coli.


Physical studies of RNA involvement in bacteriophage lambda DNA replication and prophage excision.


Requirement for transcription in the neighborhood of the phage attachment region for lysogenization of Escherichia coli by bacteriophage lambda.


Plasmid-controlled variation in the content of methylated bases in single-stranded DNA bacteriophages M13 and fd.


Rat thymus cells in culture. I. Peculiar granules in cytoplasm of reticulum cells.


Rat thymus cells in culture. II. Reticulum cells and globulins.


Association of gnotobiotic mice with various organisms isolated from conventional mice.


Effects of bacterial flora on cecal size and transit rate of intestinal contents in mice.


Amyloidosis: its significance in oral surgery. Review of the literature and report of a case.


Complete extrusion of an endodontic silver point through the body of the mandible. Report of a case.


The effects of specific auxotrophic mutations on the virulence of Aspergillus nidulans for mice.


Metastatic tumors of the oral soft tissues. Review of the literature and report of a case.


The nature of lipomas and their significance in the oral cavity. A review and report of cases.


Lysosomes and the dental pulp.

Lysosomes and the dental pulp.


William D. Coolidge. Reflections of one hundred years.


The prospects of PGs in postconceptional therapy. This is a comprehensive review of the literature dealing with the use of PGs (prostaglandins) to induce labor contractions or abortion.  Many graphed bodily responses are used to illustrate the discussion.  The functional character of the pregnant human uterus is described.  It is theorized, from a review of many studies, that the pregnant human uterus is intrinsically active, but endogenously suppressed.  Abortion or labor occur when the suppressor is removed, allowing the intrinsic stimulation within the uterus to operate.  The uterus is capable at all times of synthesizing PG; the rate of synthesis depends on the amount of stretch.  The administration of PG depends on time for its stimulating effect.  Clinical trials have been conducted with various routes of PG administration.  Current clinical studies being conducted in the field are reviewed.  Evidently, saline solution causes abortion through the same mechanism as PGs, i.e., stimulation of the myometrium.  Intrauterine PG therapy will remain the treatment of preference.  Work is also being carried out in the mobilization of endogenous PGs.


Cross-cultural research in the perception of pictorial materials.


Hypertension, natriuresis and the renomedullary prostaglandins: an overview.


Grigore Benetato. Member of the Academy of the Socialist Republic of Romania (November 18, 1905--June 9, 1972).


Cyanoacrylate in retinal surgery.

Cyanoacrylate in retinal surgery.


Expulsive hemorrhage during intraocular surgery.


Pulmonary arteriovenous fistulas in children. A review with special reference to the disperse telangiectatic type, illustrated by report of a case.


Continuous positive airway pressure with a face chamber in early treatment of idiopathic respiratory distress syndrome.


Vitreous surgery. X. Current status of vitreous surgery in cases of rhegmatogenous retinal detachment.


Nystagmus velocity computer.

Nystagmus velocity computer.


The computer in otolaryngology: what ever happened?


Trimethoprim in human saliva, bronchial secretion and lung tissue.


The nutritive value for growing pigs of single cell protein (Saccharomyces cerevisiae) produced from sulphite spent liquor.


Proteases of Clostridium botulinum. I. Classification of proteases and literature survey.


Proteases of Clostridium botulinum. II. The relationship between growth medium and the production of proteases by Clostridium botulinum types A, B, C, D, E and F.


In memoriam.

In memoriam.


The effects of Clostridium botulinum toxin type C beta given orally to goats.


The effects of peptide-precipitating lignosulphonic acids on the proteolytic activity of pepsin in vitro and on the response of pigs to an ulcer inducing diet.


Morphological and ultrastructural studies of moose spermatozoa.


The generalized Shwartzman reaction in pigs induced by diet and single injection of disintegrated cells or partially purified endotoxin from Escherichia coli.


Effects of ischemia on the contractile processes of heart muscle.


Function of the hypoxic myocardium. Experimental and clinical aspects.


Peripheral circulatory control mechanisms in congestive heart failure.


New developments and therapeutic applications of cardiac stimulating agents.


The blood volume of infants. IV. Infants born by cesarean section.


An autoradiographic investigation of epithelium-egg interaction in the mouse oviduct.


A follow-up study on prophylactic antibiotics in cesarean section.


Inactivity of rodent malaria anti-sporozoite antibodies against exoerythrocytic forms.


Isolation of Salmonella from sparrows captured in horse corrals.


Physiologic consequences of positive end-expiratory pressure (PEEP) ventilation.


Bibliography of secondary sources on the history of dermatology. I. Journal articles in English.


Fifteen-year clinical experience with the vena cava filter.


Divergent nature of gastric mucosal permeability and gastric acid secretion in sick patients with general surgical and neurosurgical disease.


Vogt-Koyanagi-Harada syndrome and psoriasis.


Materials and methods.

Materials and methods.


Ocular changes in the generalized Shwartzman reaction.


Date mistake.

Date mistake.


Eyelids, lacrimal apparatus, and conjunctiva.


The ultrastructure of the villi-like structure in Trypanosoma cruzi.


Cell surface antigen and viral antigen of Marek's disease virus in lymphoid cells of the spleen from chickens inoculated with virulent strains.


Yeast killer factor: ATP leakage and coordinate inhibition of macromolecular synthesis in sensitive cells.


Increased rates of sugar transport in Saccharomyces cerevisiae. A result of sugar metabolism.


The unsaturated fatty acid requirement in Escherichia coli. Temperature dependence and total replacement by branched-chain fatty acids.


Regulation of the intracellular potassium concentration in Escherichia coli B 525.


Value of physical examination in acute myocardial infarction.


The effect of beta-galactoside accumulation on the uptake of phosphate into cells and cell nucleotides of Escherichia coli.


Myocardial abscess due to Salmonella typhimurium.


A controlled trial of beclomethasone dipropionate for asthma.


Aerosols of salbutamol, terbutaline and isoprenaline-phenylephrine in asthma.


A comparison of pivampicillin and ampicillin in exacerbations of chronic bronchitis.


Tetracycline and co-trimoxazole in acute exacerbations of chronic bronchitis.


A double-blind trial of phenelzine and amitriptyline in depressed out-patients. A possible differential effect of the drugs on symptoms.


Trial of a sustained release form of amitriptyline (Lentizol) in the treatment of depressive illness.


Application of renal autotransplantation to the treatment of simultaneous bilateral ureteral tumours.


Drug resistance and detection of R factors among fecal Escherichia coli strains isolated from laboratory animals.


Studies with guanidines on the mechanism of K+ transport in yeast.


LH-RH interaction with the pituitary plasma membrane.


Prediction of the secondary structure of the L7, L12 proteins of the E. coli ribosome.


Characterization of the receptor protein for phage T5 and colicin M in the outer membrane of E. coli B.


Inhibition of magic spot formation by L-1-tosylamido-2-phenylethyl chloromethyl ketone.


Stoichiometry of initiation factor IF-3 in exponentially growing Escherichia coli MRE600.


The chemical synthesis of deoxyribo-oligonucleotides complementary to a portion of the lysozyme gene of phage T4 and their hybridization to phage specific RNA and phage DNA.


Quantitative determination of carbamazepine in plasma by mass fragmentography.


The Lundh test.

The Lundh test.


Some observations on the serology of Pneumocystis carinii infections in the United States. An indirect immunofluorescent (IF) test with antigen of partially cleaned Pneumocystis carinii cysts was evaluated for sensitivity and specificity. The test was reactive at the 1:8 and above level for 44% of the persons who had suspected and confirmed infections, for 7.3% of the healthy contacts, and for 1.4% of the general population. Results of tests with sera from people with measurable antibodies to other diseases and those on immunosuppressant therapy suggest that only titers of 1:20 and above are specific. At this level, tests with sera of 32% of the cases, 4% of the contacts, 0% of the healthy controls, and from 0% to 37% (average 5%) of individuals on long-term immunosuppressive therapy or with antibodies to other diseases were positive. The high percentage of positive reactions in cytomegalovirus and in fungal infections could represent double infections. The complement fixation test was less sensitive than the IF test. Antibodies measured by the IF test were immunoglobulin G. No differences in antigenicity were demonstrated by the IF test among the cysts from eight human infections or from induced rat infections.


Cell-mediated immune response in cattle to Mycoplasma mycoides var. mycoides. The cell-mediated immune response of cattle to Mycoplasma mycoides var. mycoides was studied. Sensitized lymphocytes in blood leukocyte preparations showed a significant degree of antigen-induced transformation, judged by the uptake of tritiated thymidine. The increase in tritiated thymidine uptake in sensitized lymphocytes in the presence of M. mycoides membrane antigen varied from 2- to 13-fold compared with the controls, and this increase in activity was observed from 3 days after artificial infection. Inhibition of leukocyte migration by M. mycoides membrane antigen commenced between 17 and 30 days after infection, and preliminary observations indicate that this test correlated with the intradermal allergic test. M. mycoides-induced unresponsiveness was demonstrated 23 and 30 days after infection. Unresponsiveness, in that the lymphocytes did not respond to phytohemagglutinin, was very marked in two of three animals and partial in the third animal, whereas the humoral antibody response did not appear to be affected. Antigen-induced transformation was demonstrated in only two out of six cattle vaccinated two months previously with M. mycoides T(1) broth culture vaccine, and óne animal only gave a doubtful intradermal allergic reaction. A further six cattle vaccinated 15 months previously were negative to both the leukocyte migration inhibition test and the intradermal allergic test.


Three characteristics associated with enterotoxigenic Escherichia coli isolated from man. In Dacca, Bangladesh, potent enterotoxin-producing Escherichia coli were isolated from many hospital cases of acute cholera-like diarrhea. Enterotoxigenic (tox(+)) and non-enterotoxigenic (tox(-)) isolates of E. coli were used to investigate possible means of differentiating tox(+)E. coli from those (tox(-)) of the normal flora. The majority (81%) of the tox(+)E. coli studied were found to be negative for sucrose fermentation, 85% exhibited retarded growth in a peptone medium at pH 8.5, and 92% released large amounts of ammonium sulfate precipitable materials into culture supernatant fluids; 66.6% exhibited all three of these properties. For the tox(-) group the respective values were found to be 50%, 31%, and 34%; only 9.3% exhibited all three properties. These results indicate that it may be possible to use phenotypic characteristics other than antigenic composition and enterotoxin production for the identification of enterotoxigenic E. coli.


Comparative immunology: sipunculid bactericidal responses. This report continues comparative studies of inducible bactericidins of invertebrates. The bactericidin of the sipunculid worm Dendrostomum zostericolum Chamberlain was induced by intracoelomic injections of killed bacteria. With the assay system used, optimal results were obtained with 90 min of incubation at 20 to 35 C. Bactericidal titers in coelomic fluids from noninjected worms were either low or nonexistent. After injections of gram-negative bacteria, bactericidal titers of up to 1:1280 were reached within 7 days. Considerable individual variations were noted. Optimal stimulation was achieved with 4 x 10(8) bacteria per injection. The response was relatively nonspecific as measured by cross-immunization and absorption experiments. Injections of sterile sea water produced no response. Coelomic fluids could be inactivated by heating at 50 or 56 C for 20 min. Activity was not restored by a pool of unheated coelomic fluid from nonimmunized worms. The significance of these observations was discussed with reference to immunity of sipunculids and to comparative immunology.


In vitro correlates of delayed hypersensitivity in man: ambiguity of polymorphonuclear neutrophils as indicator cells in leukocyte migration test. Delayed cutaneous hypersensitivity (DCH) of 12 normal adult subjects to purified protein derivative (PPD) of Mycobacterium tuberculosis, streptococcal streptokinase-streptodornase (SK-SD), and Candida albicans Dermatophytin O (DO) was assayed in vivo by skin testing and compared with such in vitro correlates of cellular immunity as lymphocyte transformation (LT) and inhibition of leukocyte migration (ILM) from microcapillary tubes or in agarose gel. LT was shown to be the best in vitro correlate of specific lymphocyte sensitization with all antigens. In the ILM assays, PPD showed good correlation with in vivo DCH and in vitro LT; SK-SD showed partial correlation; DO showed no correlation, not being active in any of the ILM tests. Cell distribution and morphology of stained migration patterns, ILM tests performed on separated populations of lymphocytes and polymorphonuclear leukocytes (PMN), as well as the ability of test antigens to stimulate PMN cells to reduce nitroblue-tetrazolium dye, indicated that in ILM tests mononuclear cells were not inhibited in their migration, whereas migration of PMN cells appeared to depend on their direct reaction with the test antigens.


In vitro transformation of mouse bone-marrow-derived (B) lymphocytes induced by the lipid component of endotoxin. An analysis of which component of lipopolysaccharide, the lipid or the polysaccharide, is mitogenic for mouse B-lymphocytes has been performed. A purified glycolipid derived from a rough mutant of Salmonella minnesota (R595) that does not contain any o-polysaccharide at all is more mitogenic than an intact lipopolysaccharide derived from a smooth strain of S. minnesota. Results using fractions produced by several different chemical modifications of whole lipopolysaccharide confirm this result. Acid hydrolysis separates lipopolysaccharide into two components. The lipid fraction is mitogenic, whereas the polysaccharide fraction is not. Those procedures which degrade or modify only the lipid moiety while preserving the antigenic integrity of the polysaccharide also destroy mitogenicity. These include alkaline hydrolysis and deacylation by a more specific treatment with potassium methylate. The lipid preparations are fully active on highly purified B-lymphocyte populations (prepared by anti-theta antiserum and complement), whereas they have no effect on highly purified T-lymphocyte populations (prepared by anti-immunoglobulin and complement). These data demonstrate that the lipid moiety of endotoxin is the B-lymphocyte mitogen, whereas the polysaccharide has no demonstrable mitogenic activity.