Transport of lysine and hydroxylysine in Streptococcus faecalis. Data are presented which support the view that l-lysine is transported by two systems in Streptococcus faecalis. The system with the higher affinity for l-lysine appears to be specific for l-lysine among the common amino acids and to require an energy source. The second system transports both l-lysine and l-arginine and does not appear to require an energy source. Both of these systems will accept hydroxy-l-lysine as a substrate as shown by the energy requirement for hydroxy-l-lysine transport and by the inhibition of uptake by l-arginine as well as by l-lysine. The affinity of both systems appears to be considerably lower for hydroxy-l-lysine than for l-lysine. A mutant of S. faecalis which is resistant to the growth inhibitory action of hydroxy-l-lysine appears to differ from the parent strain by having a defective l-lysine-specific transport system. In this mutant, hydroxy-l-lysine is not readily transported via the l-lysine-specific system because of the mutation or via the second system because of the high concentration of l-arginine present in the growth medium. This overall lack of transport prevents hydroxy-l-lysine from reaching inhibitory levels within the cell.


Biosynthesis of 50S ribosomal proteins during the outgrowth of Bacillus subtilis spores. The biosynthesis of 14 protein bands from 50S ribosomes was studied during the outgrowth of Bacillus subtilis W23 spores. The time at which the synthesis of each protein band begins and the patterns of synthesis that each displays were determined by comparing the incorporation of (3)H-phenylalanine during five intervals throughout the first hour of outgrowth with the incorporation of (14)C-phenylalanine between 145 and 150 min. The majority of the proteins in the 14 bands analyzed were synthesized as early as 5 to 10 min. The bands could be grouped on the basis of their labeling patterns as well as the time required for them to achieve a high rate of synthesis. Three bands did not achieve a high rate of synthesis until after 30 min of outgrowth, and at least one of the proteins from these bands appeared to be located on the exterior of the 50S subunit. The significance of the proteins from these three bands is discussed in terms of the late formation of new, mature 50S subunits during outgrowth.


Significance of altered carbon flow in aromatic amino acid synthesis: an approach to the isolation of regulatory mutants in Pseudomonas aeruginosa. Pseudomonas aeruginosa displays a native resistance to a variety of inhibitory compounds, including many analogues of amino acids, purines, and pyrimidines. Therefore, it has been difficult to isolate analogue-resistant regulatory mutants which have been so valuable in other microbial species for the study of enzyme control mechanisms and for the study of amino acid transport and its regulation. However, we have found that increased sensitivity to growth inhibition by analogues can be demonstrated by manipulation of the nutritional environment. When P. aeruginosa is grown with fructose as the nutritional source of carbon and energy, the cells become sensitive to growth inhibition by beta-2-thienylalanine and p-amino-phenylalanine, analogues of phenylalanine and tyrosine, respectively. Thus, mutants were isolated which are resistant to growth inhibition by beta-2-thienylalanine and p-amino-phenylalanine when fructose is the carbon source, and many of the beta-2-thienylalanine-resistant mutants overproduce phenylalanine. Several lines of evidence suggest that the increased sensitivity to growth inhibition by analogues of phenylalanine and tyrosine reflects a decreased rate of synthesis of aromatic amino acids or their precursors when fructose is the carbon source. This general approach promises to be valuable in the study of regulatory phenomena in microorganisms which, like P. aeruginosa, are naturally resistant to many metabolite analogues.


Molecular heterogeneity of the Bdellovibrios: evidence of two new species. A systematic examination of a variety of isolates of the bacterial endoparasite Bdellovibrio has revealed extensive molecular diversity. The quantity of deoxyribonucleic acid (DNA) polynucleotide homology ranges from more than 90% among the isolates with DNA containing 50 to 51% guanine plus cytosine (GC) to undetectable levels between the 43% GC and 51% GC isolates. The two isolates with low GC-containing DNA (H-I Bdellovibrio A3.12 and UKi2) have only 16% DNA homology. H-I Bdellovibrio A3.12 and 109 have barely detectable ribosomal ribonucleic acid (rRNA) homology, whereas the homology approaches 100% among all the high GC isolates tested. Cases of high DNA/DNA and DNA/rRNA homologies are reflected in low dissimilarities of enzyme migration patterns in starch gel electrophoresis. The dissimilarities exhibited among the high GC Bdellovibrio isolates are as low as those previously reported for different Escherichia coli strains. The zymograms of H-I Bdellovibrio A3.12 and UKi2 are completely different from each other as well as from all other bdellovibrios (100% dissimilarity). Genome sizes determined for the representative isolates demonstrate three size ranges which coincide with group differences based on the above measurements. Enzyme assays reveal that all isolates possess a tricarboxylic acid cycle and most contain an alanine and glutamic dehydrogenase. We conclude that the use of bacterial endoparasitism as a defining trait has resulted in a molecularly diverse collection of isolates. It is recommended that the specific epitaph bacteriovorus be used only for the type specimen (Bdellovibrio 100 of Stolp and Starr, 1963) and for other related 50 to 51% GC isolates. The heterogeneity of the group warrants two new species. We designate Bdellovibrio A3.12 as the nomenclatural type of B. starrii sp. n. and Bdellovibrio UKi2 as the nomenclatural type of B. stolpii sp. n.


Mode of cell wall growth of Bacillus megaterium. The growth of the cell wall of Bacillus megaterium KM was examined by labeling the cell wall uniformly with (3)H-alpha, epsilon-diaminopimelic acid and examining by radioautography the distribution of labeled cell wall material in daughter cells. The results indicate that the label is distributed uniformly to all daughter cells and rule out a single growth point, either central or apical, for these cells. The results strongly suggest that cell wall growth occurs by uniform deposition of new wall over the whole cell surface.


Gene frequency analysis of chromosomal initiation sites in Bacillus subtilis after ultraviolet light or x-ray exposure. Bacillus subtilis was exposed to ultraviolet light (UV) or X rays, and gene frequency analysis was used to study the location of initiation sites of postirradiation deoxyribonucleic acid (DNA) synthesis. It was found that DNA synthesis resumes primarily from the origin after UV exposure. With X irradiation, the origin is not selectively replicated. Elevated origin-to-terminal marker ratios observed after UV exposure of exponentially growing cells were interpreted as evidence for selective UV resistance of the replicative origin region of the bacterial chromosome.


Localization of primordia within the dorsal mesothoracic disc of drosophila.


Deoxyribonucleic acid homologies of some so-called "Hydrogenomonas" species. Evidence based on deoxyribonucleic acid homology supports the abandonment of the genus Hydrogenomonas. Pseudomonas facilis (formerly Hydrogenomonas facilis) is closely related to the nonautotrophic species P. delafieldii. P. facilis and Alcaligenes eutrophus (often called H. eutropha) are not related to each other or to other hydrogen bacteria and pseudomonads studied.


Fermentation of agmatine in Streptococcus faecalis: occurrence of putrescine transcarbamoylase. Putrescine transcarbamoylase, EC 2.1.3.x (carbamoylphosphate:putrescine transcarbamoylase), has been purified from Streptococcus faecalis 10C1 grown on agmatine as primary energy source. The formation of N-carbamoylputrescine from putrescine and carbamoylphosphate serves as a convenient and sensitive assay for this enzymatic activity. The enzyme catalyzes both the phosphorolysis arsenolysis of N-carbamoylputrescine. Arginine does not induce the synthesis of putrescine transcarbamoylase in S. faecalis. Furthermore, the putrescine transcarbamoylase activity is easily separated from ornithine transcarbamoylase activity by gel filtration on Sephadex G-100 indicating that the two activities are associated with different proteins. The significance of this new enzyme in the fermentation of agmatine and its relation to the other known transcarbamoylases are discussed.


Purification and characterization of phosphoenolpyruvate carboxylase from Plasmodium berghei. Phosphoenolpyruvate (PEP) carboxylase was purified over 400-fold from Plasmodium berghei. The purified enzyme was stable in 0.4 m potassium phosphate buffer (pH 7.4) containing 0.5 m glucose, 1 mm ethylenediaminetetraacetic acid (EDTA), and 1 mm MgCl(2). It had a molecular weight of 280,000 determined by sucrose density gradient centrifugation in this buffer, but it aggregated and was unstable in the presence of different salts or a more dilute solution of potassium phosphate. The K(m) for PEP was 2.6 mm and that for Mg(2+) was 1.3 mm. The K(m) for bicarbonate was 2 mm. Citrate, nucleotides, and EDTA inhibited the PEP carboxylase of P. berghei by decreasing the concentration of free magnesium ions, but acetyl-coenzyme A, fructose-1,6-diphosphate, and aspartate did not influence its activity. A chloroquine concentration of 1.8 x 10(-4)m inhibited the enzyme 50%.


Motility of the oviduct in the rhesus monkey. In vivo studies with and without intrauterine contraceptive devices.


Erythrocyte carbonic anhydrase B levels during pregnancy and use of oral contraceptives.


Gonorrhea--diagnosis and treatment.

Gonorrhea--diagnosis and treatment.


Phakotomy and aspiration. A new technique for lens removal through a small incision.


Galactosyltransfer in mouse mastocytoma: synthesis of a galactose-containing polar metabolite of retinol.


An organ specific ribonucleoprotein from goat brain.


Isolation and characterization of an RNA relaxed mutant of B. subtilis.


Physiological control of DNA nucleotide sequence redundancy in the eukaryote, Tetrahymena pyriformis.


Participation of rare DNA templates in DNA-RNA hybridization in the eukaryote, Tetrahymena pyriformis.


Selective amplification of repetitive DNA in the eukaryote, Tetrahymena.


Protein synthesis with isolated mitochondrial polysomes.


Reversible oxygenation of tyrosinase.

Reversible oxygenation of tyrosinase.


Biosynthesis of L-iduronic acid in heparin: epimerization of D-glucuronic acid on the polymer level.


Swine abscesses caused by Lancefield's group E streptococci. X. Specificity of the precipitin test for their detection with relation to streptococcal antigens of groups R and T, Streptococcus uberis, and Staphylococcus aureus.


Ca 2+ -specific removal of Z lines from rabbit skeletal muscle. Removal of rabbit psoas strips immediately after death and incubation in a saline solution containing 1 mM Ca(2+) and 5 nM Mg(2+) for 9 hr at 37 degrees C and pH 7.1 causes complete Z-line removal but has no ultrastructurally detectable effect on other parts of the myofibril. Z lines remain ultrastructurally intact if 1 mM 1,2-bis-(2-dicarboxymethylaminoethoxy)-ethane (EGTA) is substituted for 1 mM Ca(2+) and the other conditions remain unchanged. Z lines are broadened and amorphous but are still present after incubation for 9 hr at 37 degrees C if 1 mM ethylenediaminetetraacetate (EDTA) is substituted for 1 mM Ca(2+) and 5 mM Mg(2+) in the saline solution. A protein fraction that causes Z-line removal from myofibrils in the presence of Ca(2+) at pH 7.0 can be isolated by extraction of ground muscle with 4 mM EDTA at pH 7.0-7.6 followed by isoelectric precipitation and fractionation between 0 and 40% ammonium sulfate saturation. Z-line removal by this protein fraction requires Ca(2+) levels higher than 0.1 mM, but Z lines are removed without causing any other ultrastructurally detectable degradation of the myofibril. This is the first report of a protein endogenous to muscle that is able to catalyze degradation of the myofibril. The very low level of unbound Ca(2+) in muscle cells in vivo may regulate activity of this protein fraction, or alternatively, this protein fraction may be localized in lysosomes.


Differentiation of antibody-forming cells in toad spleen. A study using density and sedimentation velocity cell separation. Antibody-forming cells (AFC), developing in toad spleen after stimulation with polymerized flagellin, were studied with an immune adherence assay. Differentiation was followed by several parameters: thymidine uptake to monitor dividing cells; equilibrium density centrifugation in albumin gradients to monitor cell density; microscopic measurements and sedimentation velocity separation to monitor cell size; stained preparations to follow cell morphology. Almost all AFC observed early in the response were dividing cells; the proportion of dividing AFC dropped to 4% 2 wk after stimulation. The earliest AFC detected (3 days) formed a relatively homogeneous light density population, and were purified 17-fold by equilibrium density centrifugation. As the response developed, additional denser peaks were found, so that late in the response dense AFC predominated. Dividing AFC were confined to the light density region throughout the response. Cell diameter measurements revealed that the earliest AFC were all very large cells. In a manner analogous to the density changes, smaller AFC appeared as the response developed until they finally comprised the majority of the AFC population. Dividing AFC were always relatively large, but encompassed a wide range of sizes. Sedimentation velocity separation was employed in a closer study of the immature AFC; they were purified 140-fold by this procedure. The earliest AFC consisted of several readily separable size populations in the range 9-18 micro diameter. The presence of separate peaks related by factors of two in volume suggested that the largest cells undergo a series of halving divisions before entering a division growth cycle. The results suggest an AFC differentiation sequence from a very large, light density, dividing "blast" cell to a nondividing cell with the size, density, and morphological appearance of a small lymphocyte. Stages of this sequence can be defined and selected out for investigation, using sedimentation velocity and equilibrium density centrifugation as complementary techniques.


The roentgenographic diagnosis of female urethral diverticula.


Determination of B1c-B1a globulin in children.


Toxicity in the dog of inhaled  90 Y in fused clay particles: distribution, retention kinetics, and dosimetry.


Monkey psychiatrists.

Monkey psychiatrists.


Bacteriological aspects of mycobacterial infections.


Biological activity of an encephalitogenic fragment in the monkey.


The partial purification and properties of hydroxylysine kinase from rat liver.


T2r +  bacteriophage-induced enzymes. I. The purification and properties of deoxycytidylate deaminase.


The proliferation of plasma cells from mouse bone marrow in vitro. I. The role of thymus. An in vitro proliferative system for immunoglobulin-G-forming plasma cells from the bone marrow of mice was established by the addition of antigenic protein and thymic cells or their homogenate to bone marrow cultures. The promoting activity of the thymus on plasma cells was independent from mouse strain, but it differed in strength with the variations of donor strains. Synthesis of immunoglobulin-G in proliferating plasma cells and its antibody reactivity against the administered antigen were demonstrated by immunocytological analyses.


Effects of adult social experience of patterns of reproductive activity of socially deprived male rhesus monkeys (Macaca Mulatta).


Chronic allogeneic disease. 3. Genetic requirements for the induction of glomerulonephritis. The pathogenesis of glomerulonephritis in F(1) hybrid mice injected with parental spleen cells was investigated in several ways. Whenever glomerulonephritis developed, the lesion had the typical morphology produced by antigen-antibody complexes. Experiments employing backcross mice demonstrated that the antigen is supplied by the recipient and that it is specified by the H-2 gene complex, or by a locus closely linked to H-2. The source of the antibody was investigated by staining glomerular lesions with fluorescein isothiocyanate-tagged anti-immunoglobulin allotype sera. Only donor-type allotypes could be detected. The ability of the donor's immunocytes to respond to the recipient's histocompatibility antigens in such a way as to produce nephritogenic immune complexes varied from strain to strain, and seemed to be controlled by a gene unrelated to H-2. The results suggest that cell surface antigens, such as histocompatibility antigens, may be of importance in the pathogenesis of several kinds of glomerulonephritis.


Effect of an intrauterine device on menstrual cyclicity and luteal function in the baboon.


Composite resins: catching-up.

Composite resins: catching-up.


Blue Cross and forever.

Blue Cross and forever.


In vivo incorporation of (1- 14 C)leucine into rat liver and serum protein after dietary supplementation with 3'-methyl-4-dimethylaminoazobenzene, chloramphenicol or both compounds.


Relative antigenic specificities of two PVX strains and their D-protein oligomers.


Effect of nalidixic acid on the growth of deoxyribonucleic acid bacteriophages. The effect of nalidixic acid on the growth of various deoxyribonucleic acid (DNA) bacteriophages has been investigated by one-step growth experiments. The Escherichia coli bacteriophages T5, lambda, T7 and phiR are strongly inhibited by nalidixic acid, whereas T4 and T2 are only partially inhibited. The Bacillus subtilis bacteriophages SP82, SP50, and phi29 are relatively unaffected by nalidixic acid. There is no correlation between those bacteriophages which can grow in the presence of nalidixic acid and the presence of an unusual base in the phage DNA.


Clinical response to nitroglycerin as a diagnostic test for coronary artery disease.


Role of cations in milk-ejecting action of oxytocin in rat mammary tissue.


Cryoextraction of cataract: an evaluation of 132 eyes.


Alkaline phosphatases of the chicken duodenum. I. Isolation and partial characterization of the multiple forms of duodenal phosphatase in pre- and post-hatching stages.


Purification and characterization of a new type of acid carboxypeptidase from Aspergillus.


Alkaline phosphatases of the chicken duodenum. II. Enzymic dissociation of a large phosphatase complex predominant in the duodenum before hatching.


Analysis of a Bacillus subtilis proteinase mutant. A Bacillus subtilis mutant having a phenotype manifesting reduced extracellular proteolytic activity was investigated. An extracellular protein was isolated and shown by fingerprint analysis to be a fragment of the wild-type enzyme. By using previously established molecular weights for the wild-type enzyme (2.9 x 10(4)) and the two polypeptide chains derived from it (1.4 x 10(4) each), with the amino acid analysis and fingerprints of both wild-type and mutant proteins, a molecular weight of 1.57 x 10(4) was assigned to the mutant protein. (32)P-diisopropylphosphate labeling of the mutant protein showed only 1 in 53 molecules to be functional. Thin-layer chromatography on Sephadex G-75 demonstrated that the active molecules were separable from the bulk of the isolated protein and had the same mobility as the wild-type enzyme. Fingerprints of tryptic digests of (32)P-diisopropylphosphate-labeled wild-type and mutant proteins showed that the labeled peptides had identical characteristics.


Method for restricting incorporation of radioactivity from  3 H-thymidine into deoxyribonucleic acid only during outgrowth of spores of Bacillus cereus T. When heat-activated spores of Bacillus cereus T (thy(-)) were germinated and grown in medium containing (3)H-thymidine, a significant amount of radioactivity was incorporated into ribonucleic acid and deoxyribonucleic acid (DNA). A method was developed to restrict the incorporation of radioactivity from (3)H-thymidine into DNA only. This was accomplished by labeling the cells with (3)H-thymidine in the presence of 2 mg of 2-deoxyadenosine per ml, 250 mug each of uracil, cytosine, and guanosine per ml, and 500 mug of adenosine per ml. Under these conditions, 97% of the radioactivity incorporated into cold trichloroacetic acid-insoluble material was associated with DNA only. In the absence of these compounds, DNA contained only 72% of the total radioactivity incorporated into cold acid-insoluble material.


Specific effects of heating of transformable streptococci on their ability to discriminate between homospecific, heterospecific, and hybrid deoxyribonucleic acid. Heating competent bacteria of the Challis strain of Streptococcus at a temperature of 48 C causes them to lose their transformability and mainfest a slight retardation of growth rate without loss of viability. The heat-induced loss of transformability is due to diminution in the ability of the bacteria to bind deoxyribonucleic acid (DNA) irreversibly. Another effect of heat is upon a step in the transformation process subsequent to binding, a step in which DNA molecules will compete if they multiply infect an unheated cell. Despite the reduction in irreversible binding exhibited by heated cells, competition between DNA molecules to transform these cells is decreased. Neither of these sites affected by heat exhibits any specificity with regard to origin of DNA. Since heat treatment causes a relative stimulation of transformation by heterospecific DNA, a third effect of heat must be envisaged. The amount of heat-induced stimulation is dependent upon the amount of heterospecific material in the transforming DNA. Linkage of heterospecific markers is increased as a consequence of heating the recipients. Transformation by markers of different transforming efficiency in homospecific DNA is also affected by heat treatment in a differential manner. Taken together, these results point to a heat-sensitive intracellular mechanism that recognizes DNA base sequences during transformation. The effect of heat upon discrimination against heterospecific DNA has been found to occur also in the pneumococcus and in Bacillus subtilis.


Nature of deoxyribonucleic acid synthesis and its relationship to protein synthesis during outgrowth of Bacillus cereus T. Deoxyribonucleic acid (DNA) synthesis during early outgrowth of spores of Bacillus cereus T (thy(-)) has been examined. (14)C-thymidine incorporated begins 2 to 5 min after germination and continues at a slow rate up to 30 min, after which the rate of (14)C-thymidine incorporation increases considerably. Early DNA synthesis up to 30 min after germination is dependent upon simultaneous protein synthesis. The examination of the stability of proteins synthesized soon after germination shows that they are susceptible to intracellular degradation. The evidence provided here indicates that protein degradation is the cause of observed dependence of DNA synthesis on simultaneous protein synthesis. The DNA synthesis occurring soon after germination is primarily a repair type synthesis which is followed by the onset of normal replication approximately 30 min after germination.


Effect of temperature on the integrity of Bacillus psychrophilus cell walls. Cell walls isolated from Bacillus psychrophilus autolyse at temperatures which support growth. At temperatures above the maximum growth temperature (28 C), a nonenzymatic lysis occurs. Removal of autolytic enzyme activity with 10 m LiCl had little effect on the rate or extent of lysis at elevated temperatures (37 and 45 C). Nonenzymatic lysis was characterized chemically by a decrease in the liberation of N-terminal groups, and the effects of pH, Ca(2+), and ethylenediaminetetraacetic acid suggest that ionic linkages are involved in much of the integrity of the cell wall of this psychrophile. The nonenzymatic absorbance decrease at 45 C can be reversed to the extent of 70 to 100% at 0 C. Centrifugation of a heat-lysed wall suspension separated a soluble protein component which is required for low-temperature reaggregation. Preliminary evidence indicates the insoluble residue which remains after temperature-mediated lysis is primarily peptidoglycan.


Interaction of concanavalin A with the cell wall of Bacillus subtilis. Interactions between concanavalin A and cell wall digests of Bacillus subtilis 168 resulted in insoluble complexes as observed by double gel diffusion, turbidity, and analysis of the precipitate. The macromolecular constituent of the cell walls complexing with concanavalin A was the polyglucosylglycerol phosphate teichoic acid. The complex exhibited two pH optima: 3.1 and 7.4. The complex could be dissociated by saccharides which bind to concanavalin A. In contrast to concanavalin A-neutral polysaccharide complexes, formation of the concanavalin A-wall complex was inhibited by salts. It was subsequently shown that salts induce conformational changes in cell wall digests. The data suggested that for complex formation to occur a rigid rod conformation in the glucosylated teichoic acid is probably necessary. Concanavalin A can be used as a probe to study structural features of bacterial cell walls.


3-phosphoglycerate kinase from Hydrogenomonas facilis. Phosphoglycerate kinase levels in Hydrogenomonas facilis were reasonably constant whether cells were utilizing or synthesizing hexose during growth. Specific enzyme activities (micromoles of 3-phosphoglycerate disappearing per minute per milligram of protein) at 30 C were 0.234, 0.391, 0.300, and 0.229 in the "soluble" fraction derived from cells grown on fructose, lactate, succinate, and glutamate, respectively. The enzyme was purified 300-fold from succinate-grown cells. The final preparation, which was not homogenous but was free from glyceraldehyde-3-phosphate dehydrogenase and adenylate kinase, had a specific activity at 30 C of 90 mumoles of 3-phosphoglycerate per min per mg of protein. K(m) values for adenosine triphosphate (ATP), 3-phosphoglycerate, and Mg(++) were 0.16, 0.83, and 0.4 mm, respectively, at pH 7.4 and 30 C. Adenosine monophosphate (AMP) inhibited 23% at a ratio of AMP to ATP of 2.4, and the possible physiological implications of this inhibition are discussed. No evidence was found for an enzyme which catalyzes ATP-dependent conversion of 3-phosphoglycerate to 1,3-diphosphoglycerate, AMP, and phosphate.


Fatty acid composition of unicellular strains of blue-green algae. The fatty acids of 34 strains of unicellular blue-green algae provisionally assigned to the genera Synechococcus, Aphanocapsa, Gloeocapsa, Microcystis, and Chlorogloea by Stanier et al. have been chemically characterized. The strains analyzed can be divided into a series of compositional groups based upon the highest degree of unsaturation of the major cellular fatty acids. Twenty strains fall into the group characterized by one trienoic fatty acid isomer (alpha-linolenic acid), and seven strains fall into a group characterized by another trienoic acid isomer (gamma-linolenic acid). These groups in many cases correlate well with groupings based upon other phenotypic characters of the strains, e.g., deoxyribonucleic acid base composition. The assignment of a strain to a compositional group is not altered when the strain is grown under a variety of different culture conditions. All strains contain glycolipids with the properties of mono- and digalactosyldiglycerides.


Genetic control of enzyme induction in the  -ketoadipate pathway of Pseudomonas putida: deletion mapping of cat mutations. A number of spontaneous mutant strains of Pseudomonas putida, obtained by repeated selection for inability to grow with cis,cis-muconate, have been shown to carry deletions in catB, the structural gene for muconate lactonizing enzyme. These strains have been employed for deletion mapping of the genetic region containing catB and catC (the structural gene for muconolactone isomerase, the synthesis of which is coordinate with that of muconate lactonizing enzyme). All deletions that overlap mutant sites located on the left side of the genetic map, as well as the point mutations in that region, lead to a pleiotropic loss of both catB and catC activities. We propose that this region to the left of catB has a regulatory function. Although the details of regulation at the molecular level are unclear, our data indicate that catB and catC may well be controlled by a mechanism unlike any yet described by workers on enteric bacteria.


Role of metabolic energy in the transport of  -galactosides by Streptococcus lactis. Streptococcus lactis (ATCC 7962) accumulated thiomethyl-beta-galactoside (TMG) and other galactosides against concentration gradients when the cells were supplied with a metabolizable substrate, such as glucose. The accumulated TMG was free and not phosphorylated. In the absence of glucose, TMG rapidly entered the cell to a concentration equal to that of the medium. Agents that uncouple oxidative phosphorylation abolished active transport but not the carrier-facilitated entry of TMG. Evidence that the transport carriers were functional in the absence of glucose or in the presence of uncoupling agents included the demonstration of counterflow, which depends on competitive inhibition for the carrier for exit.


Transport alterations in a phosphatidylethanolamine-deficient mutant of Bacillus subtilis. A mutant of Bacillus subtilis ATCC 6051, demonstrated to be deficient in phosphatidylethanolamine, was found to differ from the parent strain in the rate of uptake of a variety of metabolites.


Visual detection of x-ray by the rhesus monkey.


Transfer effects of cue-related movement reversal in discrimination reversal learning with rhesus monkeys.


Visual identification and memory in monkeys with circumscribed inferotemporal lesions.


Pulmonary nocardiosis. case report and evaluation of current therapy.


Mixed connective tissue disease--an apparently distinct rheumatic disease syndrome associated with a specific antibody to an extractable nuclear antigen (ENA).


Autoimmune neutropenia associated with chronic active hepatitis.


Extracardiac roentgenographic abnormalities in cyanotic congenital heart disease.


Malignant mastocytoma in a cow.

Malignant mastocytoma in a cow.


Topical antimicrobial activity of 6-methoxy-1-phenazinol 5,10-dioxide, cupric complex.


Distribution of antibodies against bovine parovirus 1 in cattle and other animal species.


Percutaneous renal and hepatic biopsy in rhesus monkeys.


Cross reactions of anti-bovine luteinizing hormone serums.


Blood parasites of geese of the McConnell river, N.W.T.


Immune responses to the Rh antigen.

Immune responses to the Rh antigen.


Baroreflex sensitivity in patients on long-term haemodialysis.


Conductimetry in enzyme studies.

Conductimetry in enzyme studies.


The mechanism of reaction of methionyl-tRNA synthetase from Escherichia coli. Interaction of the enzyme with ligands of the amino-acid-activation reaction.


Critique on the K-pyroantimonate method for semiquantitative estimation of cations in conjunction with electron microscopy.


Conference on aging calls for alternatives to nursing homes.


Substituted 3-aminomethylbenzoxazoline-2-thiones as potential antibacterial agents.


The effects of disodium cromoglycate on perennial rhinitis in children.


A quantitative method for the determination of the activities of mushroom tyrosinase.


Occurrence of a kinin-like peptide in the urinary bladder of the toad Bufo marimus paracnemis Lutz.


Ureteral physiology in the monkey.

Ureteral physiology in the monkey.


Treatment of calcinosis circumscripta with probenecid. A 23-year-old woman presented with an 11-year history of peripheral joint pains and stiffness slowly progressing to the development of gross calcinosis circumscripta. She had no definite evidence of systemic scleroderma. She was first treated for one year with frequent intravenous sodium EDTA, which increased urinary calcium and enhanced negative calcium balance but did not lessen her calcinosis. She was then treated with probenecid up to 2 g daily and her calcinosis lessened steadily over three years. Plasma levels of both phosphorus and urate fell during this time.


The natural incidence of pyelonephritis in the nonhuman primate.


Vasodilator response in the ischemic kidney.


The susceptibility of the eye gnat Hippelates collusor to entomogenous bacteria and fungi.


Viruslike particles in the tissues of normal and  -irradiated Drosophila melanogaster.


Effects of volatile substances released by foliage of Abies balsamea.


Growth of Candida in casein hydrolysate solutions.


Contamination of "in-use" hyperalimentation solutions.


Multidense satellite of tobacco ringspot virus: a regular series of components of different densities.


Photosynthesis and the development of blue-green algal virus N-1.


Lysogeny of the blue-green alga Plectonema boryanum by LPP2-SPI cyanophage.


The proteins of visna virus.

The proteins of visna virus.


Medical students' knowledge of medical care organization and delivery: an exploratory study.


Transanastomotic jejunal tube feedings of newborn infants.


Assembly of gas vacuoles in a cell-free system of the blue-green alga Microcystis aeruginosa Kuetz. emend. Elenkin.


Hyperalimentation in cirrhotic patients.


The influence of culture temperature on the carotenoid composition of the blue-green alga, Anacystis nidulans.


Endotracheal gentamicin in bronchial infections in patients with tracheostomy.


Detergent worker lung--a hypersensitive pulmonary disease?


Melanosome of toad: a storehouse of riboflavin.


Immunofluorescent labeling of chromosomes with antisera to histones and histone fractions.


Immunochemical detection of a human species--specific esterase in interspecies hybrid cells.


Regulation of DNA synthesis and cell division in starved-refed synchronized Tetrahymena pyriformis HSM.


Assay for cell tumorigenicity in subhuman primates treated with antilymphocyte globulin.


Pharmacology of 3,5-diamino-1,24-triazole (guanazole). 1. Antitumor activity of guanazole.


Rh-positive blood for the Rh-negative patient.


Spring osteosynthesis: a preliminary report.


Inhibition of prostaglandin F 2a  or LH induced luteolysis in the pseudopregnant rabbit by 17 -estradiol.


A counterimmunoelectrophoresis inhibition test for the detection of hepatitis-associated antigen.


Inhibition of cold agglutinins (anti-I) by M. pneumoniae antigens.


Treatment of experimental allergic encephalomyelitis with encephalitogenic basic proteins.


Uric acid binding to serum proteins: differences among species.


Lipogenesis in monkey liver and adipose tissue.


On the demonstration of circulating human endogenous pyrogen.


Use of genetic markers to study cellular origin and development of tumors in human females.


Studies of the histidine residues of carbonic anhydrases using high-field proton magnetic resonance.


Organ and species specificity of mouse uterine alkaline phosphatase.


Purification and some chemical and physical properties of staphylococcal enterotoxin A.


The recovery and preliminary characterization of X chromosome mutants affecting imaginal discs of Drosophila melanogaster.


Studies on the regulation of plasma cholesterol levels in squirrel monkeys of two genotypes. Certain individual squirrel monkeys ("hypo-responders") are able to remain normocholesterolemic when fed diets containing cholesterol (0.5 mg/kcal). Other squirrel monkeys ("hyperresponders") when fed the same diet become hypercholesterolemic. The purpose of these studies was to identify the mechanisms which allow hyporesponders to compensate for dietary cholesterol. Using formula diets and sterol balance techniques, we have compared cholesterol absorption, synthesis, excretion, and turnover in hypo- and hyperresponding monkeys. Cholesterol absorption was essentially identical in the two groups (about 55 mg/day). Cholesterol synthesis was likewise similar in the two groups (about 35 mg/day) and there was no evidence of feedback inhibition at the level of cholesterol fed. Hyporesponders had faster turnover rates and smaller body cholesterol pools than did hyperresponders. Excretion of neutral steroids was similar for hypo- and hyperresponders and did not change with cholesterol feeding. In contrast, hyporesponders increased bile acid excretion shortly after cholesterol feeding was begun. Hyperresponders responded more slowly and to a lesser degree. It is concluded that, in this species, the mechanism of control of plasma cholesterol levels is related to the rate of conversion of cholesterol to bile acids.


On the role of the histidine moiety in the structure of the thyrotropin-releasing hormone.


Antibacterial 2,3-dihydro-2-(5-nitro-2-thienyl)-quinazolin-4(1H)-ones.


Septic complications of war wounds.

Septic complications of war wounds.


Group vs solo practice.

Group vs solo practice.


Morphogenic effects of halogenated thymidine analogs on Drosophila. II. Incorporation of  5 -bromodeoxyuridine into DNA.


The Stickler syndrome.

The Stickler syndrome.


Diagnosis of subacute sclerosing panencephalitis by a simple spinal fluid gel precipitation test for measles.


Listeria monocytogenes and Queen Anne.

Listeria monocytogenes and Queen Anne.


Familial selective IgA deficiency.

Familial selective IgA deficiency.


Adjustable dorsiflexion splint.

Adjustable dorsiflexion splint.


Experimental agents for skeletal imaging.


Determination of whole-body calcium by neutron activation analysis in vivo.


Xenogeneic renal preservation for transplantation in primates. I. Transient acute rejection response in autografts.


Immunosuppressive properties of antilymphocyte antibody isolated from a specific immunoadsorbent.


Mechanisms of increased cardiac responses to material isolated from cultures of Bacillus anthracis.


Comparison of the effects of Coxsackievirus A9 and of actinomycin D on the nucleolar ultrastructure of monkey kidney cells.


The enterohepatic circulation.

The enterohepatic circulation.


Diabetic ketoacidosis in community hospitals: management and results of therapy.


Petit mal epilepsy--a closer look at an old malady.


Thyroxine-binding globulin: characterization of the binding site with a fluorescent dye as a probe. The fluorescent dye 1,8-anilinonaphthalenesulfonate competed with thyroxine for binding to thyroxine-binding globulin. Fluorescence analysis indicated that the dye bound to the globulin in a molar ratio of 1:1 and with an association constant (at 23 degrees C) of 4.19 x10(6)M(-1), and that thyroxine bound to the globulin in a molar ratio of 1:1 and with an association constant (at 23 degrees C) of 2.35x10(10)M(-1). Displacement of globulin-bound dye by thyroxine was shown by fluorescence quenching, and displacement of globulin-bound thyroxine by dye was demonstrated by ultrafiltration.


Evolution of DNA base compositions in microorganisms.


Estimation of gestational age and birth weight in the rhesus monkey (Macaca mulatta).


Coagulation changes of hemorrhagic shock in baboons.


Mesenteric circulatory responses to hemorrhagic shock in the baboon.


Decline in pancreatic insulin release during hemorrhagic shock in the baboon.


Effect of antimicrobial therapy on lung bacterial clearance in the burned rat.


Use of pyocin 78-C2 in the treatment of Pseudomonas aeruginosa infection in mice. Purified pyocin, administered parenterally to mice infected with sensitive strains of Pseudomonas aeruginosa, afforded significant protection against two of the three strains studied.


Histamine and serotonin metabolism following massive small bowel resection.


Rapid preparation of hemagglutinins of togaviruses from infected cell culture fluids. Antigens in infected cell culture fluids can be easily concentrated by polyethylene glycol precipitation to yield suitable hemagglutinating and complement-fixing antigens for several togaviruses.


Metachromatic agar-diffusion microslide technique for detecting staphylococcal nuclease in foods. The metachromatic agar-diffusion (MAD) microslide technique was shown to detect nanogram quantities of staphylococcal thermonuclease in various foods without prior extraction, purification, or concentration.


Automatic device for the trypsinization of animal tissues. Using a simple glass device, enzymatically released monodisperse cells can be separated and isolated from tissue fragments by means of a discontinuous fluid velocity gradient.


Evaluation of the in vitro activity of tobramycin as compared with that of gentamicin sulfate. The in vitro activity of tobramycin was quantitatively compared with that of gentamicin sulfate against 195 bacterial isolates from clinical material. Tobramycin was found to be twice as active as gentamicin against isolates of Pseudomonas aeruginosa. Conversely, gentamicin proved fourfold more active than tobramycin against isolates of Serratia marcescens. Both drugs were of comparable activity against isolates of Staphylococcus aureus and the majority of the enterobacterial isolates other than S. marcescens. On the basis of the obtained data, the following criteria are proposed for the interpretation of diffusion susceptibility tests with 10-mug discs of gentamicin and tobramycin. Enterobacteriaceae and isolates of S. aureus are designated as susceptible to gentamicin and tobramycin if the zones of inhibition measure 15 mm or more in diameter; zones of 14 mm or less are indicative of resistance. Pseudomonadaceae are interpreted as sensitive to tobramycin and gentamicin if the inhibition zones measure at least 15 and 12 mm in diameter, respectively.


Stimulation of lactic streptococci in milk by  -galactosidase. Acid production in milk by lactic streptococci was stimulated by added beta-galactosidase. Both glucose and galactose accumulated rapidly in the presence of this enzyme. Glucose accumulation ceased as the culture entered the most rapid period of acid production, whereas galactose accumulation continued. In cultures without added beta-galactosidase, a low concentration of galactose accumulated in the milk, whereas glucose was not detected after 2 hr of incubation. Cultures grew and produced acid faster in broth containing glucose rather than galactose or lactose. These observations suggest that the lactic streptococci do not metabolize the lactose in milk efficiently enough to permit optimum acid production and that a phenomenon such as catabolite repression functions to allow for a preferential use of glucose over either galactose or lactose. In addition to providing the culture with a more readily available energy source, it is possible that the culture produced more acidic metabolites as a result of preferentially utilizing the glucose released by the action of the beta-galactosidase.


Comparison of fluorescent antibody induced by rubella virus in vaccinee and convalescent individuals. Specific rubella antibody detectable by indirect immunofluorescence developed in response to immunization with attenuated rubella vaccine, HPV-77, DK-12. Fluorescent antibody (FA) was found when vaccinee sera were reacted with antigens synthesized in three different acutely infected continuous cell lines: BHK-21, LLCMK-2, and RK-13. FA titers were high, and they correlated with antibody titers obtained by hemagglutination-inhibition tests. Levels of FA in vaccinated individuals were slightly lower than those found in persons recovered from natural rubella infections. Rubella FA persists a long time in convalescent individuals and appears to be maintained for at least 19 months in vaccinees.


Immunofluorescence staining of group B coxsackieviruses. Studies were conducted on the sensitivity and specificity of indirect fluorescent-antibody (FA) staining for identification of group B coxsackieviruses. Antisera produced in four different species (monkeys, rabbits, horses, hamsters) and immune ascitic fluids prepared in mice were compared for suitability in FA staining. The horse antisera showed high titers of nonspecific staining, and the rabbit antisera showed relatively low homologous FA titers. Immune reagents from monkeys, hamsters, and mice were used for homologous and heterologous testing against cell cultures infected with the various group B coxsackieviruses. Antisera or immune ascitic fluids produced in these three species showed some heterotypic and nonspecific staining at low dilutions, with the monkey antisera showing the highest heterotypic titers. However, the immune reagents could be diluted to a point where they gave no heterotypic reactivity, but still showed characteristic homotypic staining. Heterotypic staining appeared as diffuse, low-level staining of the cells, whereas homotypic staining revealed characteristic, brightly staining aggregates of viral antigen in the cytoplasm of the infected cells. By using hamster immune sera, appropriately diluted to eliminate heterotypic staining and yet give strong homotypic staining, it was possible to identify correctly 79 (93%) of 85 field strains of group B coxsackieviruses at the first passage level in BS-C-1 cells; the remainder of the strains were identified after two passages in BS-CS-1 cells. No incorrect identifications were made. A limited number of field strains of group B coxsackieviruses were passed into rhesus monkey kidney and human fetal diploid kidney cells, and these were all correctly identified by FA staining, even the strains which failed to produce a cytopathic effect in the human fetal diploid kidney cells. Two human heart and brain tissues from which coxsackievirus type B4 had been isolated failed to show homotypic FA staining in excess of nonspecific or heterotypic staining.


Effect of monosodium n-alkylsalicylates on spores of Bacillus subtilis. The effects of several monosodium n-alkylsalicylates on washed spores of Bacillus subtilis have been examined. None of the compounds was sporicidal, but in general they prevented dormancy induced by incubating the spores in water. This effect was related to the position in the ring and size of the alkyl groups substituted on the salicylic acid nucleus.


Whole-body and hair retention of cadmium in mice including an autoradiographic study on organ distribution.


Research into the physiology of maleness and femaleness. Its contributions to the etiology and psychodynamics of homosexuality.


Vertical-chamber confinement of juvenile-age rhesus monkeys. A study in experimental psychopathology.


IgE multiple myeloma: a report of the third case.


An improved radioisotope dilution assay for serum vitamin B12 using hemoglobin-coated charcoal.


Studies on the synthesis of phosphoglycerides in Escherichia coli.


Acute normovolemic hemodilution. Changes of central hemodynamics and microcirculatory flow in skeletal muscle.


Monoaminergic innervation of the aorta.

Monoaminergic innervation of the aorta.


Reactivity of the ultrastructure of the neocortex during functional excitation.


Response to thyrotropin releasing hormone (TRH) in normal man.


The concentration and binding of thyroxine in the serum of patients with the testicular feminization syndrome: observations on the effects of ethinyl estradiol and norethandrolone.


Electrokinetic studies of bacteria. I. Effect of nature, ionic strength, and pH of buffer solutions on electrophoretic mobility of Streptococcus faecalis and Escherichia coli.


Complications of infected ventriculoatrial shunt for hydrocephalus--a case report.


Thyroid secretion in the unanesthetized, stress-free rat and its suppression by pentobarbital.


Dislocation of the peroneal tendons at the ankle: an analysis of 25 ski injuries.


Effect of methotrexate on rabbits infected with Mycobacterium paratuberculosis or Dermatophilus congolensis.


Destruction of erythrocytes in monkeys (Macaca mulatta) infected with Plasmodium cynomolgi.


Kinetic studies of bovine carbonic anhydrase catalyzed hydrolyses of para-substituted phenyl esters.


Effects of phosphate and other anions on the reaction between ferrous ion and cytochrome c.


Gentamicin resistance in strains of Pseudomonas aeruginosa mediated by enzymatic N-acetylation of the deoxystreptamine moiety.


Hybridization of ribonucleic acid with unique sequences of mouse deoxyribonucleic acid.


Impaired microvascular filling after focal cerebral ischemia in monkeys.


Crystal structure of human carbonic anhydrase C.


Transferable carbenicillin resistance in Pseudomonas aeruginosa.


Phytochrome and temperature relations in Lactuca sativa L. Grand Rapids seed germination after thermo-dormancy.


Sources of contribution to the understanding of the etiology of mood disorders.


Gene dosage dependence of pigment synthesis in melanoma x fibroblast hybrids (hamster cells-mouse fibroblast-DOPA-oxidase-irradiation). Hybrids between Syrian hamster melanoma cells and mouse fibroblasts, containing one genome (1s) of each parent, produce neither melanin nor DOPA-oxidase ("extinction"). Attempts to induce loss of the fibroblast chromosomes by irradiation of the fibroblasts before fusion with melanoma cells resulted in the formation of colonies comprising pigmented hybrid cells, which contained 2s melanoma and 1s fibroblast chromosome-complements suggesting that extinction or re-expression of melanogenesis is a function of genic balance. This interpretation was confirmed by crosses between 2s melanoma cells with unirradiated 1s fibroblasts, which produced both pigmented and unpigmented hybrids. No correlation has thus far been established between karyotype and phenotype of the hybrid cells, but analysis of the karyological data suggests that the fibroblast chromosomes responsible for extinction cannot be numerous.


Action of DNA polymerase I of Escherichia coli with DNA-RNA hybrids as templates. Experiments indicating the ability of the ribo strand of a DNA-RNA template to guide polydeoxynucleotide synthesis by highly purified DNA polymerase I of E. coli (EC 2.7.7.7) are presented. With poly(rA).poly(dT) as template, poly(dT) is formed with a high efficiency, but almost no poly(dA). The specific activity of the enzyme, when tested with this template under suitable conditions, is eight times greater than that found for the poly(dA-dT) template. Single-stranded DNA fractions, with no template activity for DNA polymerase, are converted to efficient templates after their transcription by RNA polymerase. A concerted polymerization reaction, in which the action of DNA polymerase is dependent on that of RNA polymerase, can also be demonstrated with synthetic polydeoxynucleotides and single-stranded fractions of denatured DNA as templates.


In vitro synthesis of ribosomal RNA by Bacillus subtilis RNA polymerase. Two kinds of hybridization competition experiments show that Bacillus subtilis RNA polymerase synthesizes ribosomal RNA (rRNA) in vitro with B. subtilis DNA as a template. First, RNA synthesized in vitro competes with the hybridization of [(32)P]rRNA synthesized in vivo to the heavy strand of B. subtilis DNA. Second, unlabeled rRNA synthesized in vivo competes with the hybridization of [(3)H]RNA synthesized in vitro to denatured DNA or heavy-strand DNA, but not to light-strand DNA. The ability of RNA polymerase holoenzyme to synthesize rRNA in vitro is not lost after extensive purification. RNA polymerase core enzyme, however, which is missing the sigma factor, synthesizes little rRNA in vitro.RNA polymerase purified from wild-type sporulating cells synthesizes little rRNA in vitro, while the in vitro synthesis of rRNA by RNA polymerase from stationary phase cells of the sporulation-defective mutant rfr 10 is apparently unimpaired.


Vibrios in diseased and healthy calves.

Vibrios in diseased and healthy calves.


Synthesis of DNA in toluene-treated Chlamydomonas reinhardi (DNA replication-chloroplast DNA-cell cycle-electron microscopy). Toluene-treated Chlamydomonas reinhardi incorporate deoxynucleoside triphosphates (dNTPs) into DNA. Incorporation requires all four dNTPs, but requires neither ATP nor added DNA. The incorporation reaction is linear for nearly 20 min. The product of synthesis in treated cells is mainly chloroplast (beta-component) DNA. However, a small quantity of nuclear (alpha-component) DNA is also synthesized. Synchronously grown cells are most efficient in dNTP incorporation at a time in the cell cycle when chloroplast DNA is normally replicated. Toluene treatment disrupts the internal morphology of the cell, but leaves the outer membrane of the chloroplast intact.


Brucella milk ring tests on churn samples: a three-year study.


Comparison of three methods for measuring vitamin B 12  in serum: radioisotopic, euglena gracilis and Lactobacillus leichmannii.


Intestinal conversion of folinic acid to 5-methyltetrahydrofolate in man.


The effect of irradiation on intra-erythrocytic stages of Babesia major.


Glucose intolerance during decreased physical activity in man.


Disseminated intravascular coagulation in diabetes mellitus, with reference to the role of increased platelet aggregation.


Carbonic anhydrase in the developing ciliary processes of the rabbit eye.


The mechanism of the antagonism of experimentally induced ocular hypertension by polyphloretin phosphate.


Phagocytosis of bacteria by avian red cells.


Control of synthesis of reaction center bacteriochlorophyll in photosynthetic bacteria.


Osseous manifestations of Waldenström's macroglobulinemia.


Some aspects of dosimetry with a 4 MV linear accelerator.


Implantable subcutaneous feeding chamber for noncontinuous central venous alimentation.


The significance of bile in reflux esophagitis.


Parenteral hyperalimentation.

Parenteral hyperalimentation.


Interaction between 'sensitized lymphocytes' and antigen in vitro. V. Vascular permeability induced by skin-reactive factor.


In vivo and in vitro uptake of ferritin by Langerhans cells of the epidermis.


Immunochemical studies on the human skin lysozyme.


Ultrastructure of bone marrow prior to and after programmed freezing.


Dentigerous cysts of the mandible in a baboon.


Oral lesions as the initial sign in pemphigus vulgaris.


Induced apical closure of immature pulpless teeth in monkeys.


The effect of periodontal disease on the pulp.


Biologic and immunogenic properties of the M-PK-L attenuated strain of Japanese B encephalitis virus.


Serum-cholesterol, serum-triglyceride and ABO blood groups in a population of 50-year-old Danish men and women.


Activity of compound TH 270 (N-p-isobutoxyphenyl-N'-p'- -pyridyl-ethyl-phenylthiourea) on the experimental infection with Mycobacterium lepraemurium and Mycobacterium leprae.


Psychotherapy as the mitigation of uncertainties.


Disodium cromoglycate (INTAL).

Disodium cromoglycate (INTAL).


Ectopic paraarticular ossification after head injury.


Intravenous alimentation in pediatric patients.


Detection of hepatitis associated antigen by latex agglutination.


Carbenicillin as inhibitor of beta-lactamase from Pseudomonas aeruginosa.


Herpesvirus ateles, a new lymphoma virus of monkeys.


Paravertebral ossification of the cervical spine.


Australia-SH antigen: application of immunodiffusion and complement fixation tests to a consecutive series of patients with acute and chronic viral hepatitis.


Gastric diverticula: congenital and acquired.


Acute pancreatitis. An appraisal of 42 cases.


Cancer of the breast: is postoperative irradiation indicated?


Folacin in wheat and selected foods.

Folacin in wheat and selected foods.


Treatment of acute alcoholic hepatitis.

Treatment of acute alcoholic hepatitis.


Development of so-called dysraphism; with embryologic evidence of clinical Arnold-Chiari and Dandy-Walker malformations.


Haemoglobinuria due to typhoid and paratyphoid fever.


Serum ketone bodies, pyruvic acid, lactic acid and serum electrolytes in protein-calorie malnutrition.


Action and nomenclature of nitroglycerin and nitrate esters.


Failure of discriminative vocal conditioning in Rhesus monkey.


Quantitative organization in the mammillary bodies of adult cat.


Neurofibromatosis presenting as the epidermal nevus syndrome.


Von Recklinghausen's disease: a clinicopathological study.


Immunological study of the glial fibrillary acidic protein.


Characterization of osseous tissues by thermogravimetric and physical techniques.


An assessment and placement program (Extended care).


Biochemical and antigenic studies of lactobacilli isolated from deep dentinal caries. II. Antigenic aspects.


The state of the deciduous dentition of a chimpanzee fetus (Pan troglodytes).


Menstrual blood quantitation in the rhesus monkey: an experimental tool for improving intrauterine contraceptive devices (IUDS). 6 normally menstruating and 21 ovariectomized rhesus monkeys were used to measure the menstrual blood loss (MBL) during an estrous cycle.  A reliable method of quantitating MBL should provide better evaluation of the bleeding problem associated with IUDs.  The technic used in measuring the MBL and the volume of MBL are presented.  The ovariectomized animals were given 25-mcg estradiol im daily for 22 consecutive days of each month and 1.5-mg progesterone daily during the second half of the injection schedule.  These injections supported normal endometrial histology.  Intravaginal pads were used for the collection of the menstrual blood.  MBL was calculated by the Hallberg-Nillson method.  Average MBL for all menstrual periods was 2.01 ml.  This should provide a necessary parameter for evaluation of IUD-associated uterine hemorrhage.


Kansas breast study.

Kansas breast study.


Radiation therapy with a linear accelerator.


Problems in the treatment of venereal disease: bacterial resistance: allergy.


Chemoprophylaxis in medicine and surgery.


Supervoltage radiotherapy for carcinoma of the larynx the Johns Hopkins Hospital results 1961 to 1967.


Immunochemical study of fetal cord sera: detection of alpha and beta fetal serum proteins associated with cancer.


Ultrastructure of the intima of the central retinal artery.


The Navy Medical Reserve, present and future.


Psychiatric predictions and recommendations: a longitudinal study of character and behavior disorder patients.


The acute abdomen: a diagnostic review.

The acute abdomen: a diagnostic review.


Gastric acid secretion in infectious hepatitis.


Suicide gestures at Fort Leonard Wood: a follow-up study.


Acute alcoholic hepatitis.

Acute alcoholic hepatitis.


Examiner variability using criteria for evaluation of posterior restorative materials.


Oral hygiene program for totally dependent patients.


Renal failure due to acute glomerulonephritis during falciparum malaria: case report.


Paroxysmal atrial tachycardia: a presenting manifestation of hyperthyroidism: case report.


Surgeon General William Crawford Gorgas.


The Soviet health system--aspects of relevance for medicine in the United States.


Temperature changes produced by nicotine injected into the hypothalamus of the conscious monkey.


Non-ketotic hyperosmolar coma.

Non-ketotic hyperosmolar coma.


The use of cephalexin in the treatment of gonorrhea.


Evidence for derivative control of calcitonin secretion.


Androgens and the sexual behaviour of male rhesus monkeys.


Neutral steroid excretion during pregnancy in sub-human primates.


Dirty money.

Dirty money.


External jugular vein access to central venous system.


Medical care and our sick society. Let us avoid killing the patient by overtreatment.


Homology between nucleic acids of blue-green algae and chloroplasts of Euglena gracilis. Ribosomal RNA from several blue-green algae was hybridized to DNA from Euglena gracilis chloroplasts by the membrane filter procedure. This hydridization was competitive with chloroplast ribosomal RNA and indicates significant genetic homology between blue-green algae and plastids from Euglena gracilis.


Decreased deformability of Plasmodium coatneyi-infected red cells and its possible relation to cerebral malaria.


Immunochemical investigations on spore fractions of B. cereus strains isolated from cases of food poisoning.


Protective effect of cromolyn sodium on bronchial challenge tests in pollen asthma.


Antibacterial activity produced by molds commonly used in oriental food fermentations.


Survival of hematopoietic and lymphoma colony-forming cells in vivo following the administration of a variety of alkylating agents.


Immunological and cytogenetic properties of developing thyroid tumors in the rat.


Morphogenesis of two immunologically induced mouse lymphomas.


Liberation of a mouse uterus-specific protein in the serum during uterine carcinogenesis.


Fever and a gradually enlarging liver.

Fever and a gradually enlarging liver.


Mutation affecting normal colony formation in Escherichia coli K-12.


Experimental conditions which influence the transformation and transfection in Bacillus subtilis.


Three classes of immunoglobulins found in the sea turtle, Chelonia mydas.


The 'real' growth of medical school expenditures, 1963-64 to 1969-70.


Cryptococcal dermotropism in the rhesus monkey.


Immunoglobulin M: fixation of human complement by the Fc fragment. The Fc fragment [(Fc)(5)micro with a molecular weight of 342,000] of human immunoglobulin M from patients with Waldenström's macroglobulinemia has a complement fixing ability approximately 19 times greater on a molar basis than that of the parent immunoglobulin M. This fragment, which occurs naturally as a pentamer fixes human but not guinea pig complement, and this activity remains unchanged even after the fragment is reduced to the monomeric form.


Shared antigens between Mycobacterium bovis (BCG) and other bacterial species. Antigens from Mycobacterium bovis and Listeria monocytogenes bind to serums from normal rabbits as well as from those immunized with unrelated bacteria, especially intracellular parasites. Binding is to the Fab portion of immunoglobulin G and is immunologically specific.


Possibility of postmortem bacterial transmigration. New methods of evaluation.


Blastokinin in the Northern Fur Seal.

Blastokinin in the Northern Fur Seal.


An immunologic assay for blastokinin.

An immunologic assay for blastokinin.


Surface area in the Japanese monkey, Macaca fuscata.


On the mode of in vivo assembly of the cell wall of Bacillus subtilis.


Rabbit liver L-arginase. Purification, properties, and subunit structure.


Purification and properties of an avian carbonic anhydrase from the erythrocytes of Gallus domesticus.


Purification and properties of brain acetylcholinesterase (EC 3.1.1.7).


Dopamine- -hydroxylase in the rat brain: developmental characteristics.


Immunological properties of normal neural tissues.


Differences in chemical nature of the endotoxins derived from dissociants, types la and sm, of Pseudomonas aeruginosa, strain N 10.


Case for diagnosis.

Case for diagnosis.


Serum IgG in germfree, gnotobiotic and conventional mice of different ages.


Tender inguinal lymph nodes and gonococcal urethritis.


Systemic mast cell disease, malignant lymphoma, and IgG paraprotein.


The military blood programs in Vietnam.

The military blood programs in Vietnam.


Stress as a factor in the development of clinical malaria: a comparative study of malarial incidence in RVN casualties.


The electrocardiogram in hypertrophic subaortic stenosis.


A preliminary study of drug abuse among psychiatric inpatients.


Drugs and the military physician.

Drugs and the military physician.


Dyspeptic syndromes among older veterans.


Diagnostic laparotomy and splenectomy in the clinical staging of Hodgkin's disease.


The university hospital--a dilemma of the 70's.


Red blood cell mobility and whole blood viscosity changes following the administration of intravenous fat.


The metabolism of fat and carbohydrate during hemorrhagic shock in the unanesthetized subhuman primate: changes in serum levels of free fatty acids, total lipids, insulin, and glucose.


A new type of enzyme, and exo-splitting  -1,3 glucanase from non-induced cultures of Aspergillus nidulans.


Intracellular distribution of carbonic anhydrase in spinach leaves.


Atypical minimal peripapillary choroidal colobomata.


Sandfly fever: sequential changes in neutrophil biochemical and bactericidal functions.


Response of peripheral blood lymphocytes from normal, hypogammaglobulinemic and chronic lymphocytic leukemic patients.


Correlations between the concentrations of the four sub-classes of IgG and Gm Allotypes in normal human sera.


Naturally-occurring antibodies to bacillary teichoic acids.


Complement-dependent opsonization of incompatible erythrocytes by human secretory IgA.


Immunosuppression in mice injected with heterologous anti-immunoglobulin antisera.


The effects of antimalarial drugs on uptake and incorporation of macromolecular precursors by Tetrahymena pyriformis.


Changes in vascular reactivity and renal hemodynamics induced by benzyltrimethylammonium bromide (BTM).


The effect of polyphloretin phosphate on some smooth muscle actions of prostaglandins in the cat.


Immunity to Plasmodium berghei in rats: maximum levels of protective antibody activity are associated with eradication of the infection.


Effects of chloroquine on Plasmodium knowlesi in vitro.


Contamination of umbilical vessel catheters: encouraging information.


A case of liveborn triploidy (69,XXX).

A case of liveborn triploidy (69,XXX).


The long-term relationship between serum vitamin B12 and total body vitamin B12.


Liver vitamin A in protein-calorie malnutrition.


Nutrition intervention programs from a market viewpoint.


An electron microscopic study on the subcommissural organ of the monkey, Macacus fuscatus.


Compliance dose-response relationships in streptococcal pharyngitis.


The replication of the Kilham rat virus (RV) in various host systems: immunofluorescent studies.


Chromogenesis variations in strains of Pseudomonas aeruginosa growing in presence of chloramphenicol and oxytetracycline.


Asymbiotic nitrogen-fixing bacteria from the surfaces of nodules and roots of legumes.


Serum-soluble antigens of malaria: a review.


Current state of serological tests used to detect blood parasite infections.


Trypanosoma congolense: latex-fixation test for diagnosis of rabbit infections.


Babesia gibsoni: field and laboratory studies of canine infections.


Duck infectious anemia virus associated with Plasmodium lophurae.


Plasmodium: electron microscopy of antigen preparations.


Trypanosoma gambiense and T. equiperdum: characterization of variant specific antigens.


Bovine mastitis due to Bacillus cereus.

Bovine mastitis due to Bacillus cereus.


Hydronephrosis with heteroplastic bone formation in a cat.


Composition of the ruminal flora and establishment of ruminal ciliated protozoal species in isolated calves.


Tolypomycin, a new antibiotic. 3. Isolation and characterization of tolypomycin Y.


Pharmacology of pyrazinamide: metabolic and renal function studies related to the mechanism of drug-induced urate retention.


Uricosuric potency of 2-substituted analogs of probenecid.


Detectionn of staphylococcal enterotoxin by the gel diffusion test.


Ultrastructural lesions in kidneys with minimal involvement by systemic lupus erythematosus.


Mesonephroma of the uterine cervix. Submicroscopical study and comparison with fine structure of endocervical adenocarcinoma.


Levodopa-induced dyskinesia in the normal rhesus monkey.


Dynamics of spermiogenesis in Drosophila melanogaster. I. Individualization process.


Autoregulatory vasoconstriction and vasodilator drugs.


Coronary vasodilation and adrenergic receptors.


A technique for long-term intravenous feeding in unrestrained rats.


Cost and management: problems of intensive care units.


Immunosuppression by procarbazine. I. Sites of action of the drug and effect on adjuvant arthritis and circulating antibody responses.


Human erythrocyte membrane bound enzyme acetylcholinesterase.


Plasma thyrotropin (TSH) response to hypothalamic electrical stimulation and to injection of synthetic thyrotropin releasing hormone (TRH).


The effect of orally administered synthetic thyrotropin releasing factor on adenohypophyseal function.


Immunological stability of calcitonin in plasma.


Effects of hypercalcemia, hypercalcemia and calcitonin on glucose stimulated insulin secretion in man.


Increased glomerular clearance to ( 51 CR) EDTA in short-term insulin treated diabetics.


Exocrine  A in sera of children with asthma.


Standardization and stability of immunoglobulin E (IgE).


Allergenicity of enzyme detergents in guinea pigs exposed to simulated home-use conditions.


Role of antibody and complement in the immune clearance and destruction of erythrocytes. II. Molecular nature of IgG and IgM complement-fixing sites and effects of their interaction with serum. A model for the immune clearance and destruction of homologous erythrocytes has been further explored. In this model, every IgM anti-erythrocyte antibody molecule in an antibody preparation was shown to fix Cl. About 2000 IgG antibody molecules were required to form a Cl-fixing site on the guinea pig erythrocyte surface. 60 IgM complement-fixing sites per erythrocyte were required for the immune clearance of IgM-sensitized erythrocytes. This number of sites could be detected by a direct agglutination test. 1.4 complement-fixing sites were required for immune clearance of IgG-sensitized cells, a number of molecules which could not be detected by direct agglutination. This number could, however, be detected with the use of a Coombs antiglobulin reagent. Depletion of the late components of complement (C3-9) with cobra venom was associated with the loss of ability to clear IgM-sensitized cells and a marked deficit in the ability to clear IgG-coated cells. Thus, late (C3-9) components of complement as well as an early component (C4) were required for normal clearance of sensitized erythrocytes. There was no evidence that activation of the alternate pathway of complement action could lead to accelerated erythrocyte clearance. In vitro incubation of IgG and IgM-sensitized erythrocytes in fresh serum led to deposition of C3 and C4 on the erythrocyte surface. IgM-sensitized cells treated in this way had a normal survival. IgM-sensitized cells also were shown to remain Coombs positive after their release from the liver. The evidence suggests that the interaction of an IgM site with fresh serum in vitro and in vivo leads to formation of a site which allows for sequestration of cells in the liver. With continued exposure to serum components, this site is destroyed or inactivated. This serum-dependent inactivation is complement-dependent as shown by the use of EDTA-treated and C4-deficient serum. IgG complement-fixing sites are only partially inactivated by incubation in fresh serum, further emphasizing the differences in the biologic activity of IgM and IgG antibodies.


Immunological studies of Y protein. A major cytoplasmic organic anion-binding protein in rat liver. An antibody produced against rat Y protein, the major cytoplasmic organic anion-binding protein in liver, was characterized. The antibody precipitated Y protein from liver supernatant fractions and specifically removed the organic anion-binding capacity from this fraction.Y protein was detected by immunodiffusion with this antibody in the supernates of rat liver, kidney, and small intestinal mucosa and was not detected in supernates of 16 other tissues including bile and serum. Precipitation with anti-Y was not detected with supernates of liver from 20 other species, including man. Quantitative radial immunodiffusion revealed Y protein to constitute 4.5% of supernatant protein in rat liver and approximately 2% of supernatant protein in rat kidney and small intestinal mucosa. Phenobarbital administration increased the concentration of Y protein in rat liver by 280%, but not in kidney or small intestinal mucosa, and was associated with increased plasma disappearance of sulfobromphthalein sodium, indocyanine green, and bilirubin, and increased hepatic, but not kidney or small intestinal mucosal, content of these organic anions. These observations provide further evidence indicating that the concentration of Y protein is a major determinant of organic anion flux across the plasma membrane of the liver cell.Immunodiffusion and immunoelectrophoresis revealed serological identity between Y protein, cortisol metabolite-binding protein I. and the major azocarcinogen-binding protein.


Fibrinogen St. Louis: a new inherited fibrinogen variant, coincidentally associated with hemophilia A. A patient with classical hemophilia (factor VIII deficiency) was found to have a new abnormal fibrinogen (fibrinogen St. Louis). Other family members exhibited either defect alone. Fibrinogen St. Louis was inherited as an autosomal dominant and was not associated with clinical bleeding. When compared with normal fibrinogen, fibrinogen St. Louis was found to have defective fibrin polymerization and possibly a slower release of fibrinopeptides. The prolonged thrombin times were partially corrected by calcium chloride and protamine sulfate. Ultracentrifugal sedimentation, electrophoretic mobility, DEAE chromatographic pattern, carbohydrate content, N-terminal amino acids, immunodiffusion, and immunoelectrophoretic patterns and electrophoresis of reduced and alkylated fragments were all normal. In contrast to fibrinogen St. Louis, the most similar other fibrinogen variant (fibrinogen Zurich) was found to be heterogeneous by several criteria and to have reduced hexose content.


Immunoglobulins on the surface of lymphoid cells in Waldenström's macroglobulinemia. Immunofluorescence study of viable and fixed cells was performed on marrow and blood samples from 25 patients with Waldenström's macroglobulinemia. Whereas intracytoplasmic staining for IgM was restricted to the plasma cells and to a limited number of lymphocytic cells, the vast majority of the pleomorphic "lymphoid" proliferating cells in the marrow were shown to bear the monoclonal IgM on their surface. All IgM-secreting plasma cells displayed membrane positivity. Most lymphocytic proliferating cells carried the monoclonal IgM on their surface in the absence of detectable amounts of intracytoplasmic IgM. Despite the usual absence of increase in blood lymphocyte counts, a large number of circulating lymphocytes were shown to bear membrane-bound monoclonal IgM in patients with active disease. The number of fluorescent spots, their size and brightness varied greatly from cell to cell in marrow and blood samples of a given patient, and this finding is in contrast to the homogeneous fluorescent pattern observed in chronic lymphocytic leukemia. The data suggest that macroglobulinemia represents the proliferation of a clone of B cells which continues to mature and differentiate.


Platelet interaction with polymerizing fibrin. Interaction of washed pig, rabbit, or human platelets with fibrinogen was studied during its transition to fibrin using photometric, isotopic, and electron microscopic techniques. Untreated fibrinogen and fully polymerized fibrin had no detectable effect on platelets. Fibrinogen, incubated with low concentrations of reptilase or thrombin, formed intermediate products which readily became associated with platelets and caused their aggregation. Neutralization of the thrombin did not prevent this interaction. In the absence of fibrinogen, reptilase did not affect platelets. The interaction of polymerizing fibrin with platelets was accompanied by small losses of platelet constituents (serotonin, adenine nucleotides, platelet factor 4, and lactic dehydrogenase). This loss did not appear to be the result of the platelet release reaction. Inhibitors of the release reaction or of adenosine diphosphate (ADP)-induced aggregation did not prevent the interaction of platelets with polymerizing fibrin. Apyrase or prostaglandin E(1) (PGE(1)) reduced the extent of platelet aggregation by polymerizing fibrin, but the amount of protein associated with platelets was slightly increased. The interaction of polymerizing fibrin with platelets was completely inhibited by ethylenediaminetetraacetate (EDTA) or ethylene glycol bis (beta-aminoethyl ether) N, N,N',N'-tetraacetic acid (EGTA).Fibers formed in solutions of polymerizing fibrin were larger in the presence than in the absence of washed platelets, suggesting that platelets affect fibrin polymerization. The adherence of platelets to polymerizing fibrin may be responsible for the establishment of links between platelets and fibrin in hemostatic plugs and thrombi.


Functional evaluation of prolactin secretion: a guide to therapy. Stimulation and inhibition tests are proposed for evaluating prolactin secretion. Thyrotropin-releasing hormone (TRH) stimulates the release of prolactin from the pituitary. Chlorpromazine acts presumably at the hypothalamic level to increase prolactin secretion. L-Dopa (D,L-alpha-hydrazino-alpha-methyl-beta-[3,4-di-hydroxyphenyl]) has the opposite effect; it inhibits prolactin secretion and may be effective in suppressing galactorrhea.


Junctional apparatus in erythrocytes infected with malarial parasites.


Mineral oil adjuvants: biological and chemical studies.


The clinical application of adjuvant 65.


Benign oral tumors and tumor-like conditions.


Unproven methods of cancer management: Ferguson plant products.


Feedback regulation of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase from a marine bacterium, Vibrio MB22. A marine bacterium, Vibrio MB22, has been studied to determine the pattern of feedback regulation of the first enzyme unique to the biosynthesis of the aromatic amino acids, 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthetase. The crude extract was used to study response of the enzyme to various salts as well as possible feedback inhibitors. Ethylenediaminetetraacetic acid was found to be inhibitory to enzyme activity, and only CoCl(2), of the salts tested, allowed full recovery as well as apparent stimulation of the DAHP synthetase activity. The DAHP synthetase activity was inhibited solely by the aromatic amino acids, tyrosine, tryptophan, and phenylalanine, of the possible effectors tested. Further work demonstrated the existence of three isozymes of DAHP synthetase, each primarily inhibited by one of the aromatic amino acids.


The role of dial access in a cancer information service.


Acid-soluble nucleotides in an asporogenous mutant of Bacillus subtilis. An asporogenous mutant of Bacillus subtilis Sp(-)H12-3, which is considered to have a block at stage 0, showed general growth characteristics similar to those of sporulating cultures. However, a sudden increase in the total amount of acid-soluble nucleotides observed at t(2) in sporulating bacteria was completely absent in this mutant. In sporulating cells, a marked increase in two nucleotides which were identified to be uridine diphosphate (UDP)-galactose and UDP-N-acetylglucosamine was noted, whereas UDP-glucose appeared to be accumulated in the mutant cells at t(2). No unusual nucleotides were found in the strains of B. subtilis examined. The possible role of these UDP derivatives in early stages of sporulation in B. subtilis is discussed.


Effect of proteolytic enzymes on bacterial flagella. Sheared flagella of Salmonella typhimurium strains SL 870 (Nml(+)Fla(+)) and SL 871 (Nml(-)Fla(+)) were found to be susceptible to proteolytic digestion by trypsin, chymotrypsin, and Pronase. The rate of tryptic digestion was similar for the epsilon-N-methyllysine-containing and the nonmethylated flagella. Thin fibers, which appeared to originate from only one end of the flagellar filament, were formed upon trypsin digestion. The fibers were not dissociated at extremes of pH or upon heating. The amino acid composition of the purified fibers was very different from that of intact Salmonella flagellin, and the fibers did not cross-react with antiflagellin or antiflagellar antiserum. The possible significance of these findings is discussed in relation to the flagellar structure.


Ultrastructural studies on a mutant of Bacillus subtilis whose growth is inhibited due to insufficient autolysin production. The growth of Bacillus subtilis mutant betaA177 can be inhibited under special conditions in which not enough autolytic enzymes are produced for optimal growth. Electron microscopy studies show that during growth inhibition there is localized thickening of the cell wall at positions where cells bend. A model is proposed to explain this result. Rapid growth can be restored by adding lysozyme or a B. subtilis autolysin mixture to a growth-inhibited betaA177 culture. Such addition reduces the localized wall thickening and causes other changes in surface morphology which are described and discussed. Septum formation seems to be relatively less inhibited than cell elongation when lytic enzyme levels are reduced. Measurements were made demonstrating that walls at ends of cells are morphologically different from walls at sides of cells in cultures of betaA177 growing at 51 C.


New centrifugation technique for isolating enzymes from large cell structures: isolation and characterization of two Bacillus subtilis autolysins. Bacillus subtilis cell walls can be centrifuged through a linear gradient of 0 to 2 m LiCl and 10 to 25% sucrose so that different autolysins are removed by different salt concentrations and banded in separate positions as the walls pass through the gradient. Using this technique we have found that B. subtilis cell walls are isolated with two autolytic enzymes attached. One autolysin, a glycosidase, can be eluted from walls with 0.5 m LiCl, has a pH optimum between 5 and 8, is relatively heat-sensitive, and has a molecular weight of 60,000. The other autolysin, an alanine amidase, can be eluted from walls with 1.5 m LiCl, has a pH optimum around 8, is relatively heat-stable, has a molecular weight of 35,000, and is present in quantities ten times greater than the glycosidase.


Studies on phenolic steroids in human subjects. XVI. Role of the kidney in the disposition of estriol.


Accidental hypothermia: endocrine function during recovery.


Breast cancer--answers to some common questions.


James Ewing (1866-1943).

James Ewing (1866-1943).


Classics in oncology. Diffuse endothelioma of bone. James Ewing. Proceedings of the New York Pathological Society, 1921.


Evaluating new antineoplastic agents.

Evaluating new antineoplastic agents.


Virus inclusions formed by the pm 2  mutant of TMV.


Determinants of lymphocytic choriomeningitis interference.


Arenaviruses: inhibition by amantadine hydrochloride.


Tracheopleural fistula. A complication of the cuffed tracheostomy tube.


Lead encephalopathy in adults.

Lead encephalopathy in adults.


Lead and hemopoiesis. The mechanism and consequences of the erythropathy of occupational lead poisoning.


Lead poisoning in a family due to cocktail glasses.


The pathology of radiation effect of normal and neoplastic tissue.


Severe radiation injuries of the stomach, small intestine, colon and rectum.


Preoperative irradiation of rectosigmoid carcinoma including its regional lymph nodes.


A pilot study on preoperative irradiation of rectosigmoid carcinoma.


Gastrointestinal Kaposi's sarcoma: roentgen manifestations.


Integrated therapy for Ewing's sarcoma.

Integrated therapy for Ewing's sarcoma.


High dose, preoperative supervoltage irradiation for osteogenic sarcoma.


Adjacent field separation for homogeneous dosage at a given depth; data for the 8 MV (mevatron 8) linear accelerator.


Hodgkin's disease treated with radiation therapy: follow-up data and the value of laparotomy.


Melting, casting and shaping lead shielding blocks: method and toxicity aspects.


Potassium release after succinylcholine in acutely uremic monkeys.


Identification of some enteric bacteria which convert oleic acid to hydroxystearic acid in vitro.


Mechanism of fatty liver in protein deficiency. An experimental study in the rhesus monkey.


Studies on "allergoids" prepared from naturally occurring allergens. II. Evaluation of allergenicity and assay of antigenicity of formalinized mixed grass pollen extracts.


Enzyme allergy in populations exposed to long-term, low-level concentrations of household laundry products.


Hypersensitivity pneumonitis due to contamination of home humidifier.


Principles of blood grouping in apes and monkeys: human, simian, and cross-immune types.


Methodology of primate blood grouping.

Methodology of primate blood grouping.


Immunogenetic studies of rhesus monkeys. 3. Statistical analysis of relationships between antigenic (blood group) factors of rhesus monkeys.


Immunogenetic studies of rhesus monkeys. IV. Serologic and genetic tests with reagents from Wisconsin and The Netherlands.


Genetics of RhL-A system of rhesus monkeys.


Joint report of first histocompatibility workshop on primates.


Mixed leukocyte cultures in rhesus monkeys.


Response to immunization with rhesus monkey allogeneic leukocytes.


Leukocyte antigens of baboons.

Leukocyte antigens of baboons.


Lambda cold agglutinin with anti-A 1  specificity in a patient with reticulosarcoma.


Leukocyte antigens in baboons: a preliminary to tissue typing for organ grafting.


Arthrography in recent injuries of the ligaments of the ankle.


Neurofibromatosis, gigantism and subperiosteal haematoma. Report of two children with extensive subperiosteal bone formation.


Immunization with R mutants of S. Minnesota. I. Protection against challenge with heterologous gram-negative bacilli.


The requirement for serum complement in the detoxification of bacterial endotoxin.


The IgA system of the guinea pig.

The IgA system of the guinea pig.


C3b inactivator of man. 3. Further purification and production of antibody to C3b INA.


A rapid solid phase radioimmunoassay for staphylococcal B enterotoxin.


Recommended dietary allowance for vitamin E.


Fibrin degradation products in thrombo-embolic disease.


Food iron absorption in man. I. Isotopic exchange between food iron and inorganic iron salt added to food: studies on maize, wheat, and eggs.


A new provocative test for the diagnosis of the carcinoid syndrome.


A statistical analysis of radical mastectomy with adjuvant therapy in the initial treatment of carcinoma of the breast.


The trigeminal nerve in the baboon.

The trigeminal nerve in the baboon.


Volumetric composition of the monkey skeleton.


The ultrastructure of neonatal calf intestine and absorption of heterologous proteins.


The development of sexual dimorphism in the bony pelvis of the squirrel monkey.


Proton magnetic resonance studies of whole human erythrocyte membranes.


Stimulation of cell-free polypeptide synthesis by a protein fraction extracted from chick oviduct polyribosomes.


Dissociation of pancreatic ribosomes by p-hydroxymercuri( 14 C)benzoate.


The isolation of Euglena gracilis chloroplasts uncontaminated by nuclear DNA.


Immunologic studies of antihemophilic factor (AHF, factor VIII). 3. Comparative binding properties of human and rabbit anti-AHF.


Dyskeratosis congenita: relationship to Fanconi's anemia.


Experimentally induced alterations in the affinity of hemoglobin for oxygen. I. In vitro restoration of erythrocyte 2,3-diphosphoglycerate and its relationship to erythrocyte purine nucleoside phosphorylase activity in a variety of species.


Atrial pacing.

Atrial pacing.


Experimentally induced alterations in affinity of hemoglobin for oxygen. II. In vivo effect of inosine, pyruvate, and phosphate on oxygen-hemoglobin affinity in rhesus monkey.


The role of factors of portal origin in modifying hepatic regeneration.


Is the scrapie agent a viroid?

Is the scrapie agent a viroid?


Isolation and characterization of a protein from sciatic nerve myelin responsible for experimental allergic neuritis.


Volatility enhancement of thyrotropin releasing hormone for mass spectrometric studies.


A possible intermediate in the initiation of glycogen biosynthesis.


Irreversible inhibition of thymidylate synthetase by 5-formyl-2'-deoxyuridylic acid.


Purification and properties of isozymes of glutaminase from Pseudomonas aeruginosa.


Manual nephelometric methods for immunochemical determination of immunoglobulins IgG, IgA, and IgM in human serum.


Metabolism of  203 Hg administered as HgCl 2  in the dairy cow and calf.


Influence of prussian blue on metabolism of  137 Cs and  86 Rb in rats.


A case of accidental aspiration of  90 SrCl 2 .


Dosimetry of cobalt radioisotopes in man.


Metal activation of histidine ammonia-lyase. Metal ion-sulfhydryl group relationship.


Metabolic interlock. The dual function of a folate pathway gene as an extra-operonic gene of tryptophan biosynthesis.


Radiation dose to humans from  75 Se-L-selenomethionine.


Histological and radiographic study of the spheno-occipital synchondrosis in cynomolgus monkeys. Macaque Irus.


Egtved virus: demonstration of virus antigen by the fluorescent antibody technique in tissues of rainbow trout affected by viral haemorrhagic septicaemia and in cell cultures infected with Egtved virus.


Use of allantoic cells for the detection of avian infectious bronchitis virus.


Vaccinia virus: kinetics of the hemagglutination-inhibition test and preparation of hemagglutinin.


Markers for measles virus. I. Physical properties.


Sturucture of an abnormal plasma lipoprotein (LP-X) characterizing obstructive jaundice.


Comparative studies of adenovirus hexon antigens.


The stimulation of ornithine decarboxylase activity in testes of the neonatal mouse.


Biosynthesis of 2-aminoethylphosphonic acid in cell-free preparations from Tetrahymena.


Purification of ornithine decarboxylase from regenerating rat liver.


Effect of thiol compounds on melanin formation by tyrosinase.


Scanning electron microscopy tissue preparation.


Characterization of a blue mutant of Rhodospirillum rubrum.


Corneal lesions in squirrel monkeys maintained on a low-chromium diet.


Vitreous structure. II. Role of hyaluronate.


Consensual ocular hypertensive response to prostaglandin.


Macaque lens absorption in vivo.

Macaque lens absorption in vivo.


The effect of theophylline on plasma thyrotropin (HTSH) response to thyrotropin releasing factor (TRF) in man.


Relation of riboflavin deficiency to corticosteroid metabolism and red cell hypoplasia in baboons.


Studies on the exoerythrocytic stages of simian malaria. VII. Plasmodium simiovale.


Zinc-DDT interrelationships in growth and reproduction in the rat.


Preparation of a multi-species antigen of human malaria for use in the indirect fluorescent antibody test.


Demonstration of malarial antigens in the sera of Plasmodium berghei-infected mice.


Neurotropism in Costa Rican strains of Trypanosoma cruzi.


The development of Abbreviata caucasica (von Linstow) (Spirurida: Physalopteridae) in an intermediate host.


Serum levels of thyroid hormones in Hashimoto's thyroiditis.


Production of aflatoxin B 1  from defined natural cultures of Aspergillus flavus (Link).


Differentiation of neuromuscular junctions in cultures of embryonic Drosophila cells. Cultures were prepared of embryonic cells from Drosophila melanogaster. Neurons and myocytes differentiated in vitro from their respective stem cells. Electron microscopy showed that neuromuscular junctions formed where axons contacted myocytes. Electrical stimuli were applied to axons and these caused contractions of innervated myocytes. This is the first report of insect or other invertebrate neuromuscular junctions differentiating in vitro. In addition, this is the first system reported in which the neurons, myocytes, and junctions are completely differentiated in vitro from neuroblasts and myoblasts.


Test of the law of initial values in four species of primates.


Properties of viruslike particles of Penicillium chrysogenum: one double-stranded RNA molecule per particle.


Increased stability and reproducibility of Escherichia coli spheroplasts in the transfection assay of phage lambda DNA with polyethylene glycol instead of sucrose.


Biological properties of syncytium-forming ("foamy") viruses.


Comparison of the structures of blue-green algal viruses LPP-1M and LPP-2 and bacteriophage T7.


Studies on the replication of influenza virus RNA.


On the probability of detecting nonpaternity through the Rh blood-group system.


Central venous feeding: a call for caution.


Distribution of simultaneously injected androgens and estrogens in animal tissues.


Prostaglandins: their effects on the carbohydrate metabolism of myometrium from rhesus monkeys.


Analytic review: nature and origin of males with XX sex chromosomes.


Ornithine transcarbamylase from Streptococcus faecalis and bovine liver. I. Isolation and subunit structure.


Ornithine transcarbamylase from Streptococcus faecalis and bovine liver. II. Multiple binding sites for carbamyl-P and L-norvaline, correlation with steady state kinetics.


Ornithine transcarbamylase from Streptococcus faecalis and bovine liver. 3. Effects of chemical modifications of specific residues on ligand binding and enzymatic activity.


Germination of bacterial spores by calcium chelates of dipicolinic acid analogues.


Free and membrane-bound ribosomes of rat cerebral cortex. Metabolism of ribosomal and messenger ribonucleic acid.


Systemic and coronary vascular effects of the 2- and the 5-mononitrate esters of isosorbide.


Diuretic therapy in experimental right heart failure of the rhesus monkey.


Effects of mercurial diuretics on the renal tubular transport of p-aminohippurate and diodrast in the chimpanzee.


Homograft skin for the early management of difficult wounds.


Changes in immunoglobulin levels after surgical trauma.


An electron microscopic study of the effect of crude bacterial  -amylase and ethylenediaminetetraacetic acid on human tendon.


Genetic control of spermiogenesis in Drosophila melanogaster: an autosomal mutant (ms(2)3R) demonstrating failure of meiotic cytokinesis.


Genetic control of spermiogenesis in Drosophila melanogaster: an autosomal mutant (ms(2)10R) demonstrating disruption of the axonemal complex.


Some observations on the fine structure of the mitochondrial inner membrane of Tetrahymena pyriformis.


Levels of immunoglobulins in the serum of patients with carcinoma of the prostate.


Metabolism and toxicity of inhaled  144 Ce in rats.


Monoclonal gammopathies: present status.


Transcapillary exchange of protein-bound hormones from the bloodstream into the tissue fluid.


Preparation and mechanical perfusion of the isolated monkey brain.


Mathematical analysis for the interpretation of in vivo tracer infusion experiments.


Morphological evaluation of perfused tissues.


Safety precautions for performing tests for hepatitis-associated "Australia" antigen and antibodies.


On the importance of the N-5 position in flavin coenzymes. Properties of free and protein-bound 5-deaza analogs.


Intrinsic fluorescence of actin.

Intrinsic fluorescence of actin.


Further studies on ribulose 1,5-diphosphate carboxylase from Chromatium strain D.


Role of zinc (II) in the refolding of guanidine hydrochloride denatured bovine carbonic anhydrase.


Biochemical and immunological comparison of phenotypes of horse serum transferrins.


An excitatory component of the jaw opening reflex in the temporal and masseter muscles of cats and monkeys.


Lead binding to human haemoglobin.

Lead binding to human haemoglobin.


Gas chromatography of the nitrate esters of glycerol, isosorbide and isomannide.


The use of parenteral alimentation in renal failure: the effect of an intravenous fat emulsion and essential amino acids on dogs undergoing bilateral nephrectomy.


Pulmonary embolization complicating total intravenous alimentation.


The effect of surfactant in the auto-analyser.


Droplet-frozen sensitized red cells for Gm typing.


Repeated reuse of a starch gel in hemoglobin screening.


Experimental retinal detachment in the owl monkey. IX. Radioautographic study of protein metabolism.


Duane's retraction syndrome and associated congenital malformations.


Clinical infrared absorption angiography of the choroid.


A model for the study of infectious otitis externa.


Isolation and molecular weight of circular chloroplast DNA from Euglena gracilis.


Protein synthesis in vitro with Tetrahymena mitochondrial ribosomes.


Tissue specific brain S-100. A demonstration of multiple proteins.


Chemical and immunochemical studies on pregnant mare serum gonadotropin.


The action of cortisone acetate on cell-mediated immunity to infection: histogenesis of the lymphoid cell response and selective elimination of committed lymphocytes.


Comparative characteristics of "ether" and "non-ether" soluble ricketssia prowazekii antigens and electron microscopy findings on the morphology of the rickettsiae during isolation of the antigens.


Turbidimetric method for assay of nitrofuran compounds.


Publications for families of adult aphasics. A review of the literature.


Clinical research in thyroid surgery.

Clinical research in thyroid surgery.


Heroin addiction and the role of methadone in its treatment.


Some properties of salivary amylase: a survey of the literature and some observations.


Nutritional management of granulomatous colitis with perineal ulceration.


Endometrial carcinoma recurring after hysterectomy. A study of 64 cases, with observations on effective treatment modalities and implications for alteration of primary therapy.


Radiotherapy plus 5-FU compared to radiotherapy alone for inoperable and unresectable bronchogenic carcinoma.


Effect of postoperative radiotherapy on peripheral blood lymphocytes in patients with carcinoma of the breast.


Cytotoxicity and reduction of animal cell growth by Clostridium M-55 spores and their extracts.


Freeze etching of cells without cryoprotectants. The technique of spray-freeze etching was applied to unicellular organisms. The superior freezing rates obtainable with this method gave excellent cryofixation on Chlorella, Euglena, and spermatozoa without the use of antifreeze agents, and cell damage due to ice crystal formation was never observed. In many instances the resultant morphology differed significantly from that obtained from glycerol-treated, freeze-etched cells. Furthermore, viability studies of spray-frozen Chlorella compared favorably with cells frozen by other methods.


Skin permeability in vivo: comparison in rat, rabbit, pig and man.


Influence of Pityrosporum species in the generation of free fatty acids in human surface lipids.


Transitory traumatic paraplegia: electron microscopy of early alterations in myelinated nerve fibers.