Use of trypsin-modified human erythrocytes in rubella hemagglutination-inhibition testing. A method of treating human erythrocytes with trypsin has been modified and found to be an efficient and practical indicator system for the rubella hemagglutination-inhibition test. Both the trypsin-treated human cells and the widely used, newborn chicken erythrocytes were used in comparative testing of 464 selected diagnostic rubella serums. Results with each cell system were essentially the same. The trypsin treatment procedure has been found to be relatively simple, and with our limited testing has not presented any problems with reproducibility. Other advantages include the ready availability of human cells, greater intralaboratory standardization of the test by using the same donors over a long period of time, and elimination of adsorption of test sera with red blood cells.


Identification of a germination system involved in the heat injury of Bacillus subtilis spores. Bacillus subtilis A spores were injured by exposure to heat treatments of 110 to 132 C. Injury was demonstrated by the inability to form colonies on fortified nutrient agar (FNA) unless the medium was supplemented with CaCl(2) and Na(2) dipicolinate (CNA). A preliminary heat treatment fully heat-activated the spores, was not lethal, and did not prevent injury by subsequent secondary heat treatment. Exposure of heat-activated spores to 122 C reduced germination in FNA. The primary germination agents in FNA were identified, and a defined germination medium of glucose, NaCl, l-alanine, and sodium phosphate (GNAP) was developed. Germination of heat-activated spores in GNAP was equivalent to germination in FNA. Injury measured by colony formation on FNA and CNA was correlated to injury measured by reduced germination in both FNA and GNAP. Inactivation of the FNA and GNAP germination systems by secondary treatment exhibited similar kinetics. Therefore, injury expressed as the inability to form colonies on FNA involved alteration of the GNAP germination system.


Ultrahigh-temperature activation of a low-temperature Bacillus subtilis spore germination system. Exposure of Bacillus subtilis A spores to 115 to 125 C for several seconds activated a low-temperature germination system that remained dormant after a heat treatment that activated the normal l-alanine- and glucose-stimulated germination systems. The low-temperature germination system was characterized by an optimum temperature lower than that of the l-alanine or glucose germination systems (30 C versus 45 C), germination in the absence of exogenous germination stimulants, and the capacity for heat-induced deactivation and subsequent reactivation. The rates of activation at 115 to 125 C were exponential and were not influenced by a previous heat treatment that activated the l-alanine- or glucose-stimulated germination systems. Although activation of the low-temperature germination system was accompanied by suppression of l-alanine-stimulated germination, it did not appear to be a modification of the l-alanine germination system.


Effect of ultrasonic waves on the heat resistance of Bacillus cereus and Bacillus licheniformis spores. Heat resistance of Bacillus cereus and Bacillus licheniformis spores in quarter-strength Ringer solution decreases markedly after ultrasonic treatments which are unable to kill a significant proportion of the spore population. This effect does not seem to be caused by a loss of Ca(2+) or dipicolinic acid. The use of ultrasonics to eliminate vegetative cells or to break aggregates in Bacillus spore suspensions to be used subsequently in heat resistance experiments appears to be unadvisable.


Fungi isolated from flue-cured tobacco sold in Southeast United States, 1968-1970. Flue-cured tobacco leaves, from low- and middle-stalk positions, offered for sale in each of two markets, within each of five tobacco types, were evaluated for moisture content (MC) and filamentous fungi during August through October in 1968, 1969, and 1970. Alternaria alternata, Penicillium cyclopium, Aspergillus niger, Aspergillus repens, and Aspergillus flavus were most frequently isolated from cultured tissue. Other filamentous fungi that grew from the tissue included species from four genera of field fungi and seven species of storage fungi. Although the MC ranged from 11.0 to 22.5%, it averaged 16.4, 16.8, and 15.9% for samples taken in 1968, 1969, and 1970, respectively. Average populations of fungi per sample over the three years ranged from 0 to 1,528,500 colonies/g of tobacco.


Theoretical and practical aspects of freezing parasitic protozoa.


Betel quid inducement of epithelial atypia in the buccal mucosa of baboons.


Observations on the 1971 summer red tide in Tampa Bay, Florida.


Pathogenesis of steatorrhea in three cases of small intestinal stasis syndrome.


Experimental production of elevated serum folate in dogs with intestinal blind loops: relationship of serum levels to location of the blind loop.


Characterization of basement membrane antigens present in the urine of normal and nephritic rabbits.


The cardiac auto-immune system. VI. Purification and further charcterization of one of the human heart proteins which cross-react with rabbit anti-rabbit heart auto-antibodies.


Histochemical demonstration of enterokinase.


An immunochemical study of pigeon-breeder's disease. I. The aselective antigens.


Nystagmus after experimental cervical lesions.


Neurofibromatosis of the bladder in children.


The distribution of scattered electrons from an 8 MeV linear accelerator.


RPC from the AFIP.

RPC from the AFIP.


The relationship of reactions to complications in the radiation therapy of cancer of the cervix.


Synthesis of cysteine from methionine in normal adult subjects: effect of route of alimentation. Parenteral alimentation solutions free of cysteine, probably an essential amino acid for premature infants, were administered continuously to eight healthy men through catheters in the superior vena cava and through nasogastric tubes. When the preparation was administered parenterally, the plasma cystine concentration dropped markedly. When feeding was switched to the oral route, the concentration rose immediately, but returned to baseline only when a cystine-containing diet was fed. These studies indicate that the synthesis of cysteine from methionine is limited, even in the adult subject, when cystine-free diets are administered parenterally.


Nitrilotriacetate (NTA) in detergents and human health.


A direct analysis of early chick embryonic neuroepithelial responses following exposure to EDTA.


Identification of plasma proteins in the palate of fetal mice.


Experimental first-trimester amniocentesis in nonhuman primates.


Regulation of branched chain amino acid transaminase formation during the growth of Pseudomonas fluorescens UK-1.


Formation of 2-oxoisovalerate dehydrogenase in Pseudomonas fluorescens.


'It's only a sprained ankle'.

'It's only a sprained ankle'.


Waldenström's macroglobulinemia. Report of a case with pulmonary involvement and improvement in pulmonary symptoms only following chlorambucil therapy.


Gastrostomy. An evaluation of the Dragstedt method.


Serological and biochemical characteristics of Pasteurella multocida from free-flying birds and poultry.


Physical and morphological characterization of transmissible enteritis virus of turkeys.


Ribonuclease U 4 . Novel phosphotransferases catalyzing exonucleolytic degradation of ribonucleic acid.


Phytohemagglutinin of the lima bean (Phaseolus lunatus). Isolation, characterization, and interaction with type A blood-group substance.


Mixed conformations of deoxyribonucleic acid in intact chromatin isolated by various preparative methods.


Use of commercial anti-species globulin in the direct Coombs' test on chicken erythrocytes.


Agar gel diffusion precipitin test for hemorrhagic enteritis of turkeys.


Properties of the Fd fragment from rabbit immunoglobulin G.


The interaction of N,N'-dicyclohexylcarbodiimide with the energy conservation systems of the spinach chloroplast.


Effect of chlorine on the virus of hemorrhagic enteritis of turkeys.


Fine-structure studies of the avian infectious bursal agent. I. In vivo viral morphogenesis.


Purification and characterization of an intracellular dipeptidase from Mycobacterium phlei.


An attempt at the identification of the proteins involved in the incorporation of 5-S RNA during 50-S ribosomal subunit assembly.


Fowl cholera: gel diffusion precipitin test for serotyping Pasteruella multocida from avian species.


An induced mitochondrial DNA polymerase from Tetrahymena.


Serological survey of scrub typhus on monkeys imported from the Southeast Pacific area.


Immunodiffusion reaction to avian infectious bursal virus.


Genetic resistance to Marek's disease. Delineation of the response of genetically resistant chickens to Marek's disease virus infection.


Study of virulence of surviving microorganisms. 2.


Bursa of Fabricius, a source of bluecomb infectious agent.


The effect of dextran sulphate on the survival and multiplication of bacteria in the blood.


Studies on single radial immunodiffusion techniques for the quantitation of antigen and antibody.


Molecular heterogeneity effects of immunoglobulin classes in radial immunodiffusion.


Hexose infusions in Cebus monkeys: effects on uric acid metabolism.


Triiodothyronine: review of recent studies of its physiology and pathophysiology in man.


An open technique of bilateral pulvinarectomy in the monkey.


Long term control of food intake in the monkey.


Behavioral and EEG patterns of the female toad Bufo arenarum during artificial clasping.


Effects of ethanol dependence induced artificially in the rhesus monkey on the subsequent preference for ethyl alcohol.


Electrographic changes in the amygdala and hippocampus associated with biting attack.


Tuberculous lymphadenitis in South India--a histopathological and bacteriological study.


Experimental myocardial infarction in unanesthetized monkeys.


Fractionation of mammalian chromatin.

Fractionation of mammalian chromatin.


"Cerebral calcification following Pseudomonas meningitis in an infant".


"Personality".

"Personality".


Unclassified type of spondylo-epiphyseal dysplasia with macroepiphyses (report of a case).


Trypanosomiasis.

Trypanosomiasis.


Interrelation of serum vitamin B 12 , total body vitamin B 12 , peripheral blood morphology and the nature of erythropoiesis.


Pathophysiological factors of importance in protein-calorie malnutrition.


Recent advances in the immunology of schistosomiasis.


The pharyngeal pituitary in the Chacma baboon (Papio ursinus).


Dental implants: failures, sucesses, prosthetic considerations.


Continuing education in dentistry.

Continuing education in dentistry.


The "depressed" deciduous tooth.

The "depressed" deciduous tooth.


Effects of unilateral ablation of superior temporal cortex on auditory sequence discrimination in Macaca mulatta.


Retention of spatial alternation following frontal lobe resections in stump-tailed macaques.


Combination chemotherapy for advanced mammary carcinoma: a twelve-year experience.


Loss of recognition memory in rats with lesions of the fornix.


Response latencies in go, no-go discrimination performance by monkeys.


Management of ovarian carcinoma: a review of 14 years' experience at Beth Israel Hospital.


Anti-insulin actions of a bovine pituitary diabetogenic peptide on glycogen synthesis. The site(s) of action of a bovine pituitary diabetogenic peptide that produces hyperglycemia and hyperinsulinemia in vivo (dogs or humans) was investigated in vitro. When rat diaphragms were incubated with the peptide in the presence of insulin, the peptide depressed insulin-mediated (a) glucose uptake, (b) glycogen synthesis, and (c) glycogen synthase activation (conversion of D to I form). Incubation with the peptide alone resulted in small increases in (a), (b), and (c). Insulin-mediated glycogen synthase kinase inactivation was inhibited when both insulin and peptide were present (d), whereas glycogen synthase kinase activity was lowered by the peptide alone. High doses of insulin completely reversed inhibitory effects of the peptide on glycogen synthesis. Therefore, the hyperglycemic and anti-insulin properties of this peptide in vivo can possibly be explained by the partial blocking action of the peptide on insulin-mediated glucose uptake and glycogenesis.


An osmotically fragile mutant of Bacillus subtilis with an active membrane-associated phospholipase A 1 . By use of a newly developed procedure for the isolation of mutants with osmotically fragile protoplasts, a mutant of Bacillus subtilis was isolated that has a very active enzyme system for the catabolism of phospholipids via the sequential action of a phospholipase A(1) (EC 3.1.1.4) and a lysophospholipase (EC 3.1.1.5). The wild-type bacteria contain no detectable phospholipase A(1) activity, but do contain a protein that specifically inhibits the phospholipase A(1) in the mutant. This protein may play an important role in the control of phospholipid catabolism.


Origin of leg musculature during Drosophila metamorphosis. Pro- and mesothoracic leg imaginal disks of late third-instar larvae of genotypes affecting the electrophoretic mobilities of alpha-glycerolphosphate dehydrogenase (EC 1.1.1.8) and arginine kinase (EC 2.7.3.3) were transplanted into host larvae of different genotypes. The metamorphosed implants were analyzed microscopically for the presence of musculature, histochemically for the distribution of enzyme activity, and electrophoretically for determination of the phenotypes of the two muscle-marker enzymes. The results permit the conclusion that leg imaginal disks contain muscle stem-cells.


Repair of the lethal developmental defect in deep orange embryos of Drosophila by injection of normal egg cytoplasm. The eggs produced by deep orange females (dor/dor) of Drosophila melanogaster have a defect that causes early death of the deep orange embryos derived from these eggs. Ovaries transplanted from deep orange females to normal female hosts continue to produce defective eggs, showing that the dor mutation directly affects an ovarian function. In heterozygous embryos (dor(+)/dor) derived from eggs of deep orange females, the presence of a paternal dor(+) gene enables normal development to occur in about half of the embryos. Thus, the egg defect caused by the dor mutation is repairable after fertilization by the action of a dor(+) gene. The deep orange egg defect has also been repaired by injection of cytoplasm from unfertilized normal eggs into deep orange embryos at the syncitial preblastoderm stage of development. About one-third of the injected deep orange embryos were able to continue their development to an advanced stage of embryogenesis. This developmental response can serve as a bioassay for the identification of the defective component in eggs of deep orange females.


Film dosimetry of betatron electron beams for radiotherapy planning.


Hybridization of variants of chloramphenicol acetyltransferase specified by fi +  and fi -  R factors. An fi(-) type of R factor for transferable chloramphenicol resistance carries the structural gene for a novel species of chloramphenicol acetyltransferase (EC 2.3.1.99). The enzyme associated with the fi(-) plasmid is distinct from that described for fi(+) R factors. The fi(+)- and fi(-)-related variants of chloramphenicol acetyltransferase were hybridized in vivo and in vitro. Both techniques yielded only a single symmetrical heteromeric species rather than the three (A(3)B(1), A(2)B(2), and A(1)B(3)) hybrids predicted from the tetrameric (identical monomers) structure of chloramphenicol acetyltransferase.


Some observations on rosette formation in Cynomolgus monkeys.


Some critical remarks and suggestions about antilymphocyte serum.


The effects of testosterone propionate administered before birth on the development of behavior in genetic female rhesus monkeys.


Vetalar (ketamine HCI) a cataleptoid anesthetic agent for primate species.


Coenurosis in a zoo collection of gelada baboons (Theropithecus gelada).


Immunodepressive effects, produced by some extracellular metabolites from Ps. aeruginosa strains.


Epidemiology of Pseudomonas infections in a pediatric intensive care unit.


Histochemical distribution of carbonic anhydrase after ligation of the pancreatic duct. The distribution of carbonic anhydrase in the exocrine pancreas of the rat has been examined during the first 3 weeks after duct ligation. The normal pattern of positive reaction in ducts, centroacinar cells and capillaries changed, by the end of the first week, to one in which all the cells in the dilated ducts showed the enzyme. This situation persisted through the third week. By electron microscopy, the enzyme was found on the apical membranes of ductular, centroacinar and acinar cells, as well as on the surface membranes of the modified cells seen at 1 week. The significance of these findings with respect to function and cell origin is discussed.


Experimental visceral aspergillosis.

Experimental visceral aspergillosis. Studies conducted to gain insight into the pathogenesis of experimental aspergillosis indicated that mice pretreated with cortisone acetate and then injected intraperitoneally with nongerminating spores of Aspergillus flavus developed a high incidence of lethal visceral hyphal aspergillosis. A similar, high incidence of fatal infections was observed in cortisone-treated animals in which the number of peritoneal macrophages had been increased by prior injection of thioglycollate. To determine whether germination of spores within the host was important to the subsequent development of disseminated hyphal aspergillosis, germinating spores of A flavus were injected intraperitoneally into normal animals. While a similar dose of nongerminating spores, administered intraperitoneally into normal mice, induced a low incidence of lethal injection, germinating spores induced a high incidence of fatal disease associated with widely disseminated visceral hyphal aspergillosis. Our studies indicate that phagocytic cells in the peritoneal cavity of normal mice are able to ingest nonperminating spores of A flavus and kill them, preventing germination. However, the phagocytic cells are unable to cope with early germinating spores, which then continue to proliferate, leading to extensive hyphal invasion of visceral organs and death.


Cerebrospinal fluid concentrations of rifampin in meningeal tuberculosis.


Tissue localization of Australia antigen immune complexes in acute and chronic hepatitis and liver cirrhosis. In a significant percentage of examined cases of fulminant hepatitis, subacute hepatitis, chronic aggressive hepatitis, liver cirrhosis and chronic persistent hepatitis, Australia (hepatitis-associated) antigen (Au HAA) was identified in the liver and in extrahepatic locations. The several immunofluorescent patterns of Au HAA localization in hepatocytes strongly suggested various stages of Au HAA accumulation and release. Deposits of a mixture of immunoglobulins G and M and occasionally beta1C-globulin were found in the cytoplasm of Au HAA containing hepatocytes, on their plasma membranes, on or in the nuclei, in the cytoplasm of Kupffer cells and, rarely, in the sinusoids. The accompanying tissue changes were hepatocellular degeneration and necrosis. These intra- and extracellular complexes of Au HAA and immunoglobulins displayed strong affinity for guinea pig complement in the immunohistochemical complement fixation reaction. When tested by immunodiffusion in agar, IgG dissociated from these complexes by potassium thiocyanate (KSCN) treatment showed anti-Au HAA specificity. In fulminant hepatitis neither Au HAA nor immunoglobulins and complement were found in the liver. In chronic aggressive hepatitis and subacute hepatitis the amount of the Au HAA immune complexes identified in the liver was approximately inversely proportional to the extent and severity of the parenchymal lesions. In liver cirrhosis and chronic persistent hepatitis there was a positive correlation between the amount of the Au HAA immune complexes found in the liver and the degree of hepatocellular damage. The deposits of Au HAA, identified in extrahepatic locations including germinal centers of lymph nodes and spleen, kidney glomeruli and blood vessel walls, were as a rule accompanied by deposits of IgG, IgM, beta1C-globulin and fibrin. All these deposits showed strong affinity for guinea pig complement in the immunohistochemical reaction of complement fixation. Germinal center activation, chronic membraneous glomerulonephritis, panarteritis and simple arteriolar hyalinosis were found at sites of localization of these deposits.


Relapsing tuberculous meningitis complicated by late sequelae.


Binding of EDTA to DEAE-cellulose and its interference with protein determinations.


Method for acrylamide gel isoelectric focusing in insoluble brain proteins.


Lipids of protozoa: phospholipids and neutral lipids.


Diabetes mellitus--problems of keto-acidosis.


Leukocyte chromosome abnormalities in advanced nonhematopoietic cancer.


Further observations on the ultrastructure of cilia from Tetrahymena pyriformis.


Action of insect hormones at the cellular level. Morphological changes of a diploid cell line of Drosophila melanogaster, treated with ecdysone and several analogues in vitro.


Isolation of bovine adrenal cortex mitochondria from the zona glomerulosa.


Induced elimination of DNA from macronucleus of Tetrahymena pyriformis.


Preparation of radiomercury labeled 3-acetomercuri-2-methoxy-propyl-urea and comparative biological study with 3-choloromercuri-2-methoxy-propyl-urea.


Preparation, physicochemical properties and biological behaviour of a radiogold colloid stabilized with gelatine of isoelectric point 9-2.


Health practices and their costs in large industry.


Chromosomal location of mutations affecting sucrose metabolism in Bacillus subtilis Marburg.


Extraordinary incidence of electrophoretically silent genetic polymorphisms.


The marmoset as an animal model for teratological research.


Experimental induction of foetal malformation with "blighted" potato: a preliminary report.


Serological similarity of microtubule proteins.


A comparison of radioisotopic and bioassay methods of measurement of serum vitamin B 12.


Muscle glycogen and glycogen synthetase in diabetic man.


Pulmonary involvements in the initial phase of rat Masugi nephritis.


Experimental and cytopathological studies on the nucleic acid synthesis of murine hepatocytes.


Proliferations of the sarcoplasmic reticulum and the T system in denervated muscle fibers.


Ultrastructural changes of rat liver cells produced by aminoglutethimide (Elipten Ciba).


Degeneration of adrenergic neurons following guanethidine treatment: an ultrastructural study.


Endothelial glomerular cell mitosis in human glomerulonephritis: an electron microscopical observation.


The purification of hepatitis-associated antigen (HAA-SH-AU) from human serum and the preparation of a sheep anti-HAA serum.


A 30-minute preparative method for isolation of IgG from human serum.


Removal of leukocytes from whole blood and erythrocyte suspensions by filtration through cotton wool. I. Filtration technique.


Large scale preparation of macroglobulins.


Plasma fractionation methods used in Sweden.


Haemolytic disease of the newborn in a Zambian community.


The development of plasma derivatives in Australia.


A serum agglutinating human red cells exposed to EDTA.


Plasma fractionation in England and Wales.


Anti-tissue IgG antibodies and deficiency of IgA.


Immunization of Rh-negative volunteers by repeated injections of very small amounts of Rh-positive blood.


A survey of the production of plasma derivatives for clinical use.


Platelet factor 3 in Glanzmann's thrombasthenia.


The distribution pattern of radioactive ferrioxamine administered intravenously in rats. I.


Daily requirements and physiologic efficiency of intrinsic factor in man.


Atypical (monomyelocytic) myelogenous leukemia. Cytochemical, electron microscopic, and biochemical investigation.


Familial leukaemia.

Familial leukaemia.


Purification of haptoglobin and   2 -macroglobulin from human serum.


Ornithine decarboxylase in mouse placenta assayed by a paper disc method for  14 CO 2  capture.


A modified microtome for slicing polyacrylamide gels.


The influence of canavanine, oxygen, and urea on the steady-state levels of nitrogenase in Anabaena flos-aquae.


Carbon utilization patterns in the heterotrophic blue-green alga Chlorogloea fritschii.


The electrophoretic characterization of ribosomes from the blue-green alga Anabaena flos-aquae.


Photosynthetic and dark carbon metabolism in unicellular blue-green algae.


Sulfide utilization by purple nonsulfur bacteria.


Respiration, peroxidase, and diphenol oxidase isozymes in reproductive and nonreproductive growth of Glomerella magna.


Adenovirus serum neutralization in micro-tissue cultures of rhesus monkey kidney cells.


Comparative studies on the protective potential of antisera directed against four antigenic preparations from Pseudomonas aeruginosa.


Use of sublingual nitroglycerin in congestive failure following acute myocardial infarction.


Relationship of anginal symptoms to lung mechanics during myocardial ischemia.


Failure of intracoronary nitroglycerin to alleviate pacing-induced angina.


Coexistent idiopathic hypertrophic subaortic stenosis and coronary arterial disease.


Anemia in early infancy.

Anemia in early infancy.


Protein preference in protein-malnourished monkeys.


Effects of adenosine on isolated coronary vascular smooth muscle.


Septum and behavior: a review.

Septum and behavior: a review.


Human chorionic somatomammotropin (HCS) plasma levels in normal and pathological pregnancies and their correlation with the placental function.


Biosynthesis in vitro of immunoglobulins in primary immunological deficiencies.


Plasma radioactivity following simultaneous oral administration of intrinsic factor-bound and free radioactsis  agammaglobulinemia immunology immunologic deficiency syndromes immunology  iga igm intestinal mucosa immunology antibody foive vitamin B 12 .


New carrier protein(s) of folic acid in human serum.


DNA synthesis during human eosinopoiesis.


The effect of persantin on the phosphate compounds in erythrocytes during blood conservation.


Platelet function studies in factor V deficiency.


Cryoglobulinemia. Report of a case with  M and  G serum gel.


Porcine parvovirus: properties and prevalence of a strain isolated in the United States.


Delayed hypersensitivity reactions in Marek's disease virus-infected chickens.


Posttransfusion hepatitis after exclusion of commercial and hepatitis-B antigen-positive donors.


Origin of infection in acute nonlymphocytic leukemia. Significance of hospital acquisition of potential pathogens.


Acute intoxication with 5,5-diphenylhydantoin (Dilantin) associated with impairment of biotransformation. Plasma levels and urinary metabolites; and studies in healthy volunteers.


Prevention of reformation of peritoneal adhesions. Effect of oxyphenbutazone, proteolytic enzymes from carica papaya, and dextran 40.


A clinical evaluation of the benefits of a course of oral penicillin following periodontal surgery.


Sensory and motor nerve conduction velocity in long-term diphenylhydantoin therapy.


Structural relationship of biotin-containing enzymes. Acetyl-CoA carboxylase and pyruvate carboxylase from yeast.


Partial purification and characterization of glyoxalase I from porcine erythrocytes.


Deficiency in initiation factors of protein synthesis in stationary-phase Escherichia coli.


Dissociation and reassociation of globin-synthesizing polyribosomes from immature avian red cells.


The effect of ATP upon the oxygen sensitivity of nitrogenase from Azotobacter chroococcum.


Antitumor activity of protein moiety of the Pseudomonas aeruginosa endotoxin.


Recalcitrant pain syndromes of the food and ankle: evaluation of the lateral dorsal cutaneous nerve.


Foot pathology associated with tuberous sclerosis and neurofibromatosis.


Further observations on the properties of dog C-reactive protein and the C-reactive protein response in the dog.


An improved radioimmunoassay of triiodothyronine in serum: its application to clinical and physiological studies.


Premitotic labeling and inflammation in the gingiva of Rhesus monkeys.


Protein deficiency and carbohydrate tolerance of the infant squirrel monkey (Saimiri sciureus).


Patella cubiti--developmental or traumatic?


Hypertension associated with vascular lesion of neurofibromatosis.


Uptake and metabolism of catecholamines by the human red blood cell.


Ureteral colic from a fungus ball: unusual presentation of systemic aspergillosis.


Bacterial inhibition of ureteral smooth muscle contractility. I. The effect of common urinary pathogens and endotoxin in an in vitro system.


Major issues in national health insurance.


Prolonged partial asphyxia: effects on fetal brain water and electrolytes.


Constancy of hand preference following forebrain bisection in monkeys.


Status epilepticus of late adult onset.

Status epilepticus of late adult onset.


Diencephalic sclerosis secondary to cerebral electroshock in the marmoset monkey. A pilot study.


The topography of impaired microvascular perfusion in the primate brain following total circulatory arrest.


Study of antimicrobial activity of chamomile oil.


Protein synthesis in pox-infected cells treated with interferon.


Serological relationship of viruses and their reassembly products.


Anomalous behavior of the coat proteins of potato virus X and cactus virus X during electrophoresis in dodecyl sulfate-containing polyacrylamide gels.


Eclipse of viral DNA infectivity in SPO1-infected Bacillus subtilis.


Antitumor effects of interferon.

Antitumor effects of interferon.


Genetic studies in relation to kuru. II. Blood-group genetic patterns in kuru patients and populations of the Eastern Highlands of New Guinea.


Lingual thyroid associated with Zenker's and vallecular diverticula. Report of a case and review of the literature.


Fine morphology of the tectorial membrane. Its relationship to the organ of Corti.


Susceptibility of Neisseria gonorrhoeae to seven antibiotics in vitro. One hundred consecutive isolates of N. gonorrhoeae were tested for susceptibility to penicillin, ampicillin, tetracycline, erythromycin, kanamycin, cephaloridine and cephalexin by an agar dilution method. Relative resistance to penicillin was frequent. For 39% of isolates the minimum inhibitory concentration (MIC) of penicillin was 0.05 U./ml. or less; in 55% the MIC was 0.5 to 2.0 U./ml. Ampicillin was slightly more active than penicillin G: all isolates were inhibited by 0.5mug./ml. or less. Resistance to tetracycline and erythromycin was frequent with MIC of 1 mug./ml. or greater observed in 32 and 24% of isolates respectively. The MIC of kanamycin for all gonococci was 8 mug./ml. or greater. Cephalexin was slightly more active than cephaloridine, though each drug exhibited a wide range of MIC values. Gonococcus isolates resistant to penicillin (MIC of 1.0 U./ml. or greater) tended to be resistant to the other antibiotics tested.


Identification and quantitation of immunoglobulins.


Identification and characteristics of the common alpha 1 -antitrypsin phenotypes.


Hyperalimentation in pulmonary surgery.

Hyperalimentation in pulmonary surgery.


Dietary factors affecting biliary lipid secretion in the rhesus monkey. A mechanism for the hypocholesterolaemic action of polyunsaturated fat?


Serogenetic investigations on malignant melanomas with reference to the incidence of ABO system, Rh system, Gm, Inv, Hp and Ge systems.


A program for review of community nursing-home utilization.


Methionine transfer ribonucleic acid from folate-sufficient and folate-deficient Streptococcus faecalis R.


Interference by unsusceptible aquatic animals with the capacity of the miracidia of Schistosoma mansoni Sambon to infect Biomphalaria glabrata (Say) under field-simulated conditions in St. Lucia, West Indies.


Purification and properties of  -ketoglutarate synthase from a photosynthetic bacterium.


Federal medical chiefs on progress and plans. Department of Defense.


Federal medical chiefs on progress and plans. Army Medical Department.


Federal medical chiefs on progress and plans. Navy Medical Department.


Federal medical chiefs on progress and plans. Veterans Administration Health Care System.


Federal medical chiefs on progress and plans. Air Force Medical Service.


Pharmacists--a wasted natural resource?

Pharmacists--a wasted natural resource?


Case for diagnosis.

Case for diagnosis.


Haves and have-nots in the world of disease.


Relationship between human alpha-galactosidase isozymes.


The effect of allogeneic demineralized dentin as a pulp capping agent in Java monkeys.


Metabolism of folate coenzymes in the developing chick embryo.


Response of the hypophyseal-testicular axis in monkeys to stalk-median eminence extract.


Metabolism of the nonhepatic splanchnic area in baboons and the effects of endotoxin.


Skin-sensitizing antibodies in Schistosoma mansoni infected baboons: evidence for the presence of two types.


Studies of morphology and immunofluorescence of Pneumocystis carinii.


Carcinoma in a urethral diverticulum.

Carcinoma in a urethral diverticulum.


Intravenous hyperalimentation in pediatrics.


Automated colorimetric determination of penicillin in fermentation samples using a molybdoarsenic acid-mercuric chloride reagent.


Absorption of iron in protein-calorie deficient rats and immediate effects of re-feeding an adequate protein diet.


Transcription of genetic information and its regulation by protein factors.


Cooperative conformational changes in globular proteins.


Longitudinal studies of lipoid nephrosis.


Acquired type 3 hyperlipoproteinemia. Report of three cases associated with systemic lupus erythematosus and diabetic ketoacidosis.


Different responses to two hypothalamic hormones in acromegalics.


An outbreak of mastitis due to Pseudomonas aeruginosa in a dairy herd.


The regulation of the butanediol cycle in Bacillus subtilis.


Rhesus isoimmunization and twins.

Rhesus isoimmunization and twins.


Metabolism of aflatoxin B 1  in rhesus monkeys.


Effects of protease inhibitors on growth of hamster tumor cells in culture.


Bacteriological and clinical evaluation of the combination of cloxacillin with ampicillin.


Substituted penicillin amides. Duration of antibacterial activity (depot effect) in experimental infections in mice.


Effect of non-ionic polymers on radioallergoimmunosorbent reactions.


Thymus dependence of the antibody response in chickens: variation with dose of antigen and type of antibody affected.


Modification of antibodies. I. The effect of the new modifying agent, dodecylsuccinic anhydride, on properties of IgG immunoglobulins.


Social organization of the Guinea baboon, Papio papio.


Study of spontaneous behavior in squirrel monkey groups: observation techniques, recording devices, numerical evaluation and reliability tests.


Learning-set formation and transfer in rhesus and talapoin monkeys.


Effect of deafening on the vocal behavior of the squirrel monkey (Saimiri sciureus).


Red cell and serum proteins of Cercocebus, Presbytis, Colobus and certain other species.


Studies on gonococcus infection. II. Freeze-fracture, freeze-etch studies on gonocci. Gonococci have been studied by electron microscopy after freeze-cleavage, freeze-etching and the findings correlated with those obtainable through thin sectioning and negative staining. The outer membrane of the cell wall is composed of round to hexagonal subunits 80 A in diameter. This membrane is also punctuated by 80-A holes visible on the exterior of the organism and extending into the substance or through the outer membrane. Pili coursing over the surface of the organisms appear to maintain a close anatomical relationship with the cell wall. In some instances, the surfaces of the organisms are virtually covered by a layer of pili.


The ribosomal proteins of Drosophila melanogaster. II. Comparison of protein patterns of ribosomes from larvae, pupae and adult flies by two-dimensional polyacrylamide gel electrophoresis.


Genetic redundancy in yeast: non-identical products in a polymeric gene system.


Regional cerebral blood flow measured by intracarotid injection of hydrogen. Comparison of regional vasomotor capacitance from cerebral infarction versus compression.


Deprivation syndrome or protein-calorie malnutrition.


Atherogenicity of peanut oil in the rabbit.


Polystyrene spherules in coastal waters. Polystyrene spherules averaging 0.5 millimeter in diameter (range 0.1 to 2 millimeters) are abundant in the coastal waters of southern New England. Two types are present, a crystalline (clear) form and a white, opaque form with pigmentation resulting from a diene rubber. The spherules have bacteria on their surfaces and contain polychlorinated biphenyls, apparently absorbed from ambient seawater, in a concentration of 5 parts per million. White, opaque spherules are selectively consumed by 8 species of fish out of 14 species examined, and a chaetognath. Ingestion of the plastic may lead to intestinal blockage in smaller fish.


Transfer factor: delayed hypersensitivity to Schistosoma mansoni and tuberculin in Macaca mulatta. Delayed hypersensitivity in Macaca mulatta infected with either Schistosoma mansoni or mycobacteria was demonstrated by biopsies of skin test sites. Both dialyzable and nondialyzable leukocyte extracts from infected donors transferred delayed hypersensitivity to recipient monkeys. In two recipients, skin test conversion was associated with in vitro transformation of the recipients' lymphocytes.


Haemolysis in monkeys with Plasmodium knowlesi malaria.


Inhibition of Pseudomonas aeruginosa by ascorbic acid-sulphamethoxazole-trimethoprim combination.


Bone changes due to pulses of direct electric microcurrent.


Mastocytes, autofluorescent and silver reducing elements in bronchial carcinoids.


Differences in ultrastructural organization of amyloid as revealed by sensitivity or resistance to induced proteolysis.


Collision-tumour of the cardia.

Collision-tumour of the cardia.


An unlinked modifier of Rh blood groups: effects when heterozygous and when homozygous.


A study comparing three techniques for the detection of HAA.


Lactobacillus casei response to pteroylpolyglutamates.


Micro method for determination of blocked NH 2 - terminal amino acids of protein: application to identification of acetylserine of phosphoglycerate kinase and pyroglutamic acid of glucose 6-phosphate dehydrogenase.


Twenty-four-hour rhythmicity in carbonic anhydrase activities of choroid plexuses and pineal gland.


Enzymatic hydroxylations with molecular oxygen.


Synthesis of oligodeoxynucleotides with 5'-terminal phosphate groups.


Further evidence on multiple-peak epistasis in Drosophila melanogaster.


Expression of tox +  gene carried by an early mutant (DNA negative mutant) of nonlysogenizing phage   hv  in nontoxinogenic diphtheria bacilli.


The genetics of bacteriophage SPO1.

The genetics of bacteriophage SPO1.


The characterisation of membrane proteins by centrifugation and gel electrophoresis. A comparison of proteins prepared by different methods.


Proteins and glycoproteins of the milk fat globule membrane.


Effect of increasing concentrations of salt on the lysosomes of rat liver and Tetrahymena pyriformis.


Risk factors in the pathogenesis of atherosclerotic heart disease and generalized atherosclerosis.


Polychlorinated biphenyls--environmental impact. A review by the Panel on Hazardous Trace Substances, March 1972.


Covalently bound flavin in D-6-hydroxynicotine oxidase from Arthrobacter oxidans. Purification and properties of D-6-hydroxynicotine oxidase.


Are serum lipoproteins esterases? Esterase activity of the immunoprecipitates of duck-serum high-density lipoprotein in agar immunoelectrophoresis and immunodiffusion.


Regulation of the catabolic ornithine carbamoyltransferase of Pseudomonas fluorescens. A study of the allosteric interactions.


Regulation of the catabolic ornithine carbamoyltransferase of Pseudomonas fluorescens. A comparison with the anabolic transferase and with a mutationally modified catabolic transferase.


Mercury in fish--some toxicological considerations.


Rationalization of health services: political and social assumptions.


Serum proteins in patients with psittacosis infections.


Health planning and the U.S. federal government.


Management of esophageal achalasia in Miniature Schnauzers.


Comparison of hepatitis associated antigen and its antibody in patients with hepatitis or blood disorders.


Factors controlling the resealing of the membrane of human erythrocyte ghosts after hypotonic hemolysis.


Cation transport and electrogenesis by Streptococcus faecalis. I. The membrane potential.


Miniature end-plate currents and potentials generated by quanta of acetylcholine in glycerol-treated toad sartorius fibres. 1. Part of the end-plate region of glycerol-treated toad sartorius muscle fibres in a hypertonic solution was voltage-clamped using two microelectrodes. The control was adequate for recording miniature end-plate currents (m.e.p.c.s) in the vicinity of the electrodes only, at clamp potentials from - 20 to - 100 mV. At any potential, the peak amplitude of m.e.p.c.s varied widely but their mean amplitude was linearly related to clamp potential. The equilibrium potential, obtained by extrapolation, was more positive than in normal fibres.2. The growth phase of m.e.p.c.s was linear and rapid (< 0.7 msec). The decay phase was exponential. The time constant of decay of m.e.p.c.s was affected by temperature, and increased as the membrane potential was increased. At 20 degrees C, the time constant of decay ranged from 0.8 to 3.8 msec at membrane potentials from - 20 to - 100 mV.3. The mean conductance change caused by a quantum of acetylcholine was 5.5 x 10(-8) mho.4. Voltage responses to rectangular current injections through one electrode were recorded with three other electrodes in the end-plate region of glycerol-treated fibres. Miniature end-plate potentials (m.e.p.p.s) were also recorded with the same four electrodes.5. The decrement of both DC voltage responses and m.e.p.p.s along a fibre was exponential but the m.e.p.p. ;space constant' was significantly shorter than the DC space constant.


The Sydney funnel-web spider (Atrax robustus). 1. A review of published studies on the crude venom.


Neuropathology of systemic circulatory arrest in adult monkeys.


An improved method for isolation of rat testis Golgi apparatus.


Ten-year experience with high titer anti-D plasma for the prevention of Rh isoimmunization.


Symposium on 'manipulation of rumen fermentation'. Chairman's introduction.


Dietary effects on the secretion and actions of growth hormone.


Physiological characteristics of rumen microbes and relation to diet and fermentation patterns.


Protein synthesis and breakdown and amino acid catabolism in protein-calorie malnutrition.


Availability of some iron compounds to the veal calf.


Seven generations of protein-calorie deficiency in rats.


Immunosuppressive agents: generation and consequences of differing dose-response curves.


To what extent is male infertility genetically determined?


Anterior urethral diverticulum.

Anterior urethral diverticulum.


Arthritis and viral hepatitis. A patient with transient serum hepatitis-associated antigen, skin nodules, rash, and low serum complement.


The effect of disodium cromoglycate on pulmonary function in asthma.


Selective deficiency of cytotoxic B lymphocytes in man.


Specialisation broadens the view. The significance of a C.S.F. pulse.


Serum complement after orthotopic transplantation of the human liver.


Serum immunoglobulin levels in thyroid disease.


An analysis of DNA-containing particles recovered from the cytoplasm of differentiating chick-muscle cells.


Protein A from Staphylococcus aureus. Acetylation of protein A with acetylimidazole.


Magnetic circular dichroism of tryptophanyl residues in proteins. Azurin and carbonic anhydrases.


Protein A isolated from Staphylococcus aureus after digestion with lysostaphin.


The effect of embryonic bursectomy on the rosette-forming cells in the chicken.


Specific adsorption of cytotoxic thymus-processed lymphocytes (T cells) on glutaraldehyde-fixed fibroblast monolayers.


Specificity of antigen recognition by normal thymus cells in nude mice.


Stimulation of release of pinolysosomal contents of macrophages by nicotine.


Placental transfer and fetal distribution of mescaline- 14 C in monkeys.


Obstructive jaundice in macroglobulinemia.


The effect of deep hypothermia and circulatory arrest on the distribution of systemic blood flow in rhesus monkeys.


The value of serial colony counts and the use of dipslides as an aid in screening specimens from catheterized patients.


Hepatitis--associated antigen in aboriginal groups in Northern Australia.


Australia antigen: a review with emphasis on its clinical relevance in Australia.


A serological study of strains belonging to Sphaerophorus necrophorus, Sph. varius, and Sph. freundii.


Two unusual organisms, Aspergillus terreus and Metschnikowia pulcherrima, associated with the lung disease of ankylosing spondylitis.


Medical tax tips.

Medical tax tips.


Biosynthesis and metabolism of 1,4-diaminobutane, spermidine, spermine, and related amines.


Experimental spinal cord trauma. A historical review.


In vitro transcription.

In vitro transcription.


Levodopa therapy effects on natural history of Parkinsonism.


Technetium in cryogenic cerebral injury and edema.


Effects of so-called iron absorption-promoting additives in humans as measured with the  59 Fe-absorption whole-body retention test. Intraindividual comparison of succinate, dioctylsulfosuccinate-, fumarate-, aspartate-, ascorbate- and folate-effects on  59 Fe-absorption from therapeutic  59 Fe 2+ -doses in persons with normal and depleted iron stores.


Hemoglobin biosynthesis in murine virus-induced leukemic cells in vitro: structure and amounts of globin chains produced.


Surface bound immunoglobulins as a cell marker in human lymphoproliferative diseases.


Primary and secondary myelofibrosis: its relationship to "PNH-like de defect".


A quantitative method for investigating the bacteriology of skin: its application to burns.


The immunization of guinea-pigs and mice with a whole-culture extract of a smooth and a rough strain of Brucella abortus.


The kinetics of carbon clearance in rabbits stimulated by a tubercle bacillary lipid. A statistical model for carbon clearance studies.


Short term metabolism of  241 Am in the adult baboon.


The metabolism of  253 Es in mice.

The metabolism of  253 Es in mice.


Whole-body counting of  241 Am and  252 Cf with the anger camera.


Antigenic variation and protective immunity in Plasmodium knowlesi malaria.


Immunochemical identification of mouse IgE.


Antigen-antibody complexes in the immune response. I. Analysis of the effectiveness of complexes on the primary antibody response.


The effect of organophosphorus inhibitors, p-nitrophenol and cytochalasin B on cytotoxic killing of tumour cells by immune spleen cells, and the effect of shaking.


The activity of homologous and heterologous anti-allotypic sera in transformation and allotypic suppression in rabbits.


Atypical immunoglobulins associated with spontaneous lymphomas in an inbred strain of mice.


Recovery of nerve conduction after a pneumatic tourniquet: observations on the hind-limb of the baboon. A small pneumatic cuff inflated around the knee was used to produce tourniquet paralysis in baboons. A cuff pressure of 1,000 mm Hg maintained for one to three hours produced paralysis of distal muscles lasting up to three months. Nerve conduction studies showed that most of the motor fibres to the abductor hallucis muscle were blocked at the level of the cuff and that they conducted impulses normally in their distal parts. There was a significant correlation between the duration of compression and that of the subsequent conduction block. When tested two to three weeks after the tourniquet, the amplitude of the response of m. abductor hallucis to nerve stimulation distal to the cuff was usually slightly reduced compared with the precompression figure. This was assumed to mean that a small proportion of the motor fibres had undergone Wallerian degeneration as a result of compression. Maximal motor conduction velocity was reduced in recovering nerves. It was also reduced when a cuff pressure of 500 mm Hg was used, which was insufficient to produce persistent conduction block. In such cases a reduced velocity without evidence of block could be demonstrated 24 hours after compression. Ascending nerve action potentials were recorded from the sciatic nerve in the thigh, with stimulation at the ankle. Before compression the fastest afferent fibres had a significantly higher velocity than the fastest motor fibres in the same nerve trunk. Results after compression suggested that the high-velocity afferent fibres had a susceptibililty to the procedure similar to that of the fastest motor fibres.


Fluid volume and albumin kinetics occurring with major surgery.


A determination of the efficiency of uptake of SP50 DNA by competent cells of B. subtilis.


Impaired microvascular filling after focal cerebral ischemia in the monkey. Modification by treatment.


Long-term physical illness in childhood: a challenge to psychosocial adaptation.


Thrombocytopenia as evidence of local thrombus: the umbilical arterial catheter.


Evidence for different dispositions of particles associated with freeze-etched membranes. Brief report.


Intravenous alimentation and septicemia.


The deltopectoral flap for reconstruction of irradiated cancer of the head and neck.


Iodination of penicillinase isoenzymes.

Iodination of penicillinase isoenzymes.


Studies on the ultraviolet-sensitive mutants of blue-green alga Synechocystis aquatilis Sanv.


A cell-free system for protein synthesis from newly fertilized eggs of Drosophila melanogaster.


The effect of temperature and substrate concentration on the kinetics of (6- 3 H)uridine incorporation into RNA of toad bladder epithelial cells: the role of aldosterone.


The Rubinstein-Taybi syndrome. Report of 7 cases.


The anatomy of the corpus callosum determined electrically during stereotactic stimulation in man.


Primates as guinea-pigs.

Primates as guinea-pigs.


Dose- and time-dependent changes in the binding capacity of IgM antibody.


Experimental modification of the dominance relations of a melanotic tumour gene in Drosophila melanogaster.


Serum immunoglobulin levels in nude mice.


Fine structure of the heart of the owl monkey (Aotus trivirgatus) infected with human malaria (Plasmodium falciparum).


Chromosomal location of a prophage in Pseudomonas aeruginosa strain PAO.


Bacteria antagonistic to Pseudomonas tolaasii and their control of brown blotch of the cultivated mushroom Agaricus bisporus.


The effects of alpha-tocopherol on the growth of Tetrahymena pyriformis.


Reappraisal of the toxicology of ethylene glycol. IV. The metabolism of labelled glycollic and glyoxylic acids in the rhesus monkey.


Punished and unpunished operant behavior after atropine administration to the VMH of squirrel monkeys.


The radiographic significance of undocumented rectal bleeding.


The requirement for calcium in infection with Lactobacillus phage.


An unusual case of psoas muscle ossification due to metastatic carcinoma.


Electron microscopy of the sinoatrial node of the squirrel monkey Saimiri sciureus.


Deoxyribonucleic acid polymerase activity in a deoxyribonucleic acid polymerase I-deficient mutant of Bacillus subtilis infected with temperature bacteriophage SPO2. Increased deoxyribonucleic acid (DNA) polymerase activity is found in soluble extracts from a polymerase I-negative mutant of Bacillus subtilis after infection with temperate phage SPO2, or after induction of SPO2 prophage in lysogenic derivatives of this mutant. No increased enzyme activity is found after SPO2 infection in the presence of chloramphenicol. Infection of the polymerase-negative mutant with the DNA-negative sus mutant SPO2 L244 gives no increased enzyme activity, whereas infection with DNA-negative sus mutant SPO2 J385 gives enzyme activities comparable to those found in wild-type infected cells. These findings suggest that SPO2 determines a DNA polymerase activity essential for synthesis of phage DNA.


Clinical experience with fast neutrons.

Clinical experience with fast neutrons.


Radiobiological aspects of fast neutron therapy.


Problems with neutron therapy.

Problems with neutron therapy.


Antigens specific for human lymphocytic and myeloid leukemia cells: detection by nonhuman primate antiserums. Primate antiserums to human leukemia cells can detect antigens specific for lymphocytic leukemia cells or antigens present on certain myeloid leukemia cells. The antigen specific for lymphocytic leukemia cells is destroyed by treatment with neuraminidase or trypsin. Tryptic digests of lymphocytic leukemia cells contain the antigen, which has a high molecular weight.


Selection for circadian eclosion time in Drosophila melanogaster. Early and late eclosion strains were developed from Drosophila melanogaster cultures. The Oregon-R parent strains (isolated in 1925) showed significantly more selectability than the W(2) parent strain collected at the beginning of this study (1971). This is consistent with the hypothesis that the selective advantage of circadian behaviors is reduced in laboratory conditions.


Divergent effects of antihypertensive therapy on cardiovascular responses and left ventricular function during upright exercise.


Determination of chromium in biological materials by atomic absorption spectrometry using a graphite furnace atomizer.


The application of product inhibition studies to one substrate--one product enzymic reactions. A graphical procedure is presented for distinguishing between possible mechanisms of one substrate-one product enzymic reactions.


Inhibition of pig kidney diamine oxidase by substrate analogues. 1. The oxidation of p-dimethylaminomethylbenzylamine was followed spectrophotometrically by measuring the change in E(250) caused by the p-dimethylaminomethylbenzaldehyde produced. 2. This reaction was inhibited by substrate analogues such as isothiouronium, guanidino, dimethylsulphonium and trimethylammonium compounds. 3. The inhibition by both mono- and bis-onium compounds has been studied and a comprehensive theory is developed to explain both the type and degree of inhibition produced.


Substrate specificity of a highly active dipeptidase purified from monkey small intestine.


The role of mitochondria in modifying calcium-sensitive cytoplasmic metabolic activities. Modification of pyruvate kinase activity. 1. The modification of pyruvate kinase activity in vitro was examined by altering the environmental [Mg(2+)]/[Ca(2+)] ratio with EDTA on the one hand and isolated rat liver mitochondria on the other. 2. Controlled additions of Ca(2+) and EDTA caused pyruvate kinase activity to be alternately and rapidly switched on and off. 3. By being able to accumulate Ca(2+) in preference to Mg(2+) rat liver mitochondria were able to alter the [Mg(2+)]/[Ca(2+)] ratio in the vicinity of pyruvate kinase and thereby modify the activity of this enzyme. 4. The possible role of mitochondria in modifying pyruvate kinase and other ion-sensitive cytoplasmic enzyme activities is discussed.


Isolation of active polyribosomes from the cytoplasm, mitochondria and chloroplasts of Euglena gracilis. 1. A procedure is described for the isolation of intact polyribosomes from the cytoplasm, chloroplasts and mitochondria of Euglena gracilis. 2. All three polyribosomal preparations incorporated labelled amino acids in a system in vitro. The cytoplasmic system was inhibited by chcloheximide but not by chloramphenicol. Both the chloroplast and the mitochondrial systems, however, were inhibited by chloramphenicol but not by cycloheximide. It is shown that mitochondrial polyribosomes, like the polyribosomes from cytoplasm and chloroplasts, can participate directly in protein synthesis without supplementary mRNA being added to the synthesizing system, as in previously reported instances. 3. Sedimentation coefficients were measured for the ribosomes, ribosomal subunits, and rRNA of the cytoplasm, chloroplasts and mitochondria. 4. The G+C content was 55% for cytoplasmic rRNA, 50% for chloroplast rRNA, and 29% for mitochondrial rRNA. 5. The cytoplasmic ribosomal subunits contained a ribonuclease activity that was inhibited by heparin.


Affinity chromatography: interactions between sepharose-linked and soluble  -globulin.


Studies on human thyroxine-binding globulin. VII. The effect of environmental changes on the fluorescence of I,8-anilinonaphthalene sulfonic acid bound to thyroxine-binding globulin.


Polypeptides non-covalently associated in 19-S thyroglobulin.


Conformational studies on the constant region halves of heavy and light chains of human immunoglobulin G.


Urinary osmolarity and excretion of sodium, calcium and magnesium in patients with renal calculi.


Concealed anterior urethral diverticulum causing urinary obstruction in the male child.


Effects of Aroclor 1242 (a polychlorinated biphenyl) and DDT on cultures of an alga, protozoan, daphnid, ostracod, and guppy.


UV irradiation of Aroclor 1254.

UV irradiation of Aroclor 1254.


Effects of added or substituted potassium ion on  45 Ca movements in rabbit aortic smooth muscle.


RNA-degrading activities in chicken liver.


Recreation renews interest in life.

Recreation renews interest in life.


Experimental-anatomical studies on the "middle temporal visual area (MT)" in primates. I. Efferent cortico-cortical connections in the marmoset Callithrix jacchus.


Changes in the dorsal lateral geniculate nucleus of the squirrel monkey after unilateral ablation of the visual cortex.


Relationship between the septum and the cingulate gyrus in Macaca mulatta.


Analysis of mutagen specificity Drosophila melanogaster.


Diabetic ketosis with gross hyperlipoproteinaemia and followed by severe peripheral neuropathy.


Has the mental hospital got a future?

Has the mental hospital got a future?


Efficacy and safety of lithium carbonate in treatment of manic depressive illness.


The effect of temperature on the catalytic activity of bovine carbonic anhydrase.


Functional aspects of the three-dimensional structure of the active site of carbonic anhydrase.


Carbonic anhydrase, red cell membranes, and CO 2  transport.


Effect of chlorthalidone binding on the electrophoretic properties of human red cell carbonic anhydrase isozymes.


Preliminary clinical trials with cyanate.


Congenital anomalies in Duane's retraction syndrome.


Experimental bacterial endophthalmitis.

Experimental bacterial endophthalmitis.


Peroxidase diffusion processes in the optic nerve.


Cytotoxic lymphocytes in Hashimoto's thyroiditis.


Antibody titres of antilymphocytic sera and globulins determined by immunofluorescence and their correlation with immunosuppressive activity.


Proteolytic conversion in vitro of B. subtilis vegetative RNA polymerase into the homologous spore enzyme.


Importance of different levels of potassium in the diet on whole-body  40 K count and muscle and blood potassium.


Metabolic disposition of primidone and its metabolites in epileptic subjects after single and repeated administration.


Return to nursing  19. Rhesus immunisation.


Carbonic anhydrase isoenzymes in infants with respiratory distress syndrome.


Cortisol secretion in acidotic and nonacidotic diabetes mellitus.


Trisomy D and the cyclops malformation.

Trisomy D and the cyclops malformation.


Sudden infant death syndrome.

Sudden infant death syndrome.


Polymorphism of erythrocyte G-6-PD in the baboon.


The subclavian route. A critical review of the world literature up to 1970.


Royal farm virus: a new tickborne group B agent related to the RSSE complex.


Structural principles of biomembranes.

Structural principles of biomembranes.


Energy metabolism in mitochondria.

Energy metabolism in mitochondria.


Self-organization of molecular systems and evolution of the genetic apparatus.


Computer model of the formation of self-organizing systems.


New dihydroxyboryl-substituted polymers for column-chromatographic separation of ribonucleoside-deoxyribonucleoside mixtures.


Malate-lactate transhydrogenase.

Malate-lactate transhydrogenase.


Chirooptical properties of the cystine disulfide bond.


A direct comparison of two unusual R-factor-mediated  -lactamases.


Cell-free biosynthesis of precursors of polyprenyl derivatives of p-hydroxybenzoate in mammalian and bacterial systems.


Studies on the cell membrane of bacilli.


Lipid composition, permeability and structure of biological membranes.


Tryptophan metabolism in micro-organisms.


Microbiology of the viscous scum formed by certain Indian sewages and sewage effluents.


Localization and interconversion of tetrahydropteroylglutamates in isolated pea mitochondria. 1. Mitochondria were extracted from 4-day-old pea cotyledons and purified on a sucrose density gradient. 2. Microbiological assay of the purified mitochondrial fraction with Lactobacillus casei (A.T.C.C. 7469), Streptococcus faecalis (A.T.C.C. 8043) and Pediococcus cerevisiae (A.T.C.C. 8081) revealed a discrete pool of conjugated and unconjugated derivatives of tetrahydropteroylglutamic acid. 3. Solubilization and chromatographic studies of the mitochondrial fraction demonstrated the presence of formylated and methylated derivatives, 10-formyltetrahydropteroylmonoglutamic acid, 5-formyltetrahydropteroylmonoglutamic acid and 5-formyltetrahydropteroyldiglutamic acid being the major derivatives present. 4. The principal mitochondrial pteroylglutamates were labelled when dry seeds were allowed to imbibe [2-(14)C]pteroylglutamic acid and 5-[methyl-(14)C]-methyltetrahydropteroylmonoglutamic acid. 5. The ability of isolated mitochondria to catalyse oxidation and reduction of tetrahydropteroylglutamic acid derivatives was demonstrated in feeding experiments in which [(14)C]formaldehyde, [3-(14)C]serine, sodium [(14)C]formate, 5-[methyl-(14)C]methyltetrahydropteroylmonoglutamic acid or [2-(14)C]-glycine served as C(1) donor. In addition, (14)C was incorporated into free amino acids related to C(1) metabolism.


Biochemical evaluation of pesticides.

Biochemical evaluation of pesticides.


Aortic lipolytic enzymes in atherosclerosis.


Protein synthesis in Mycobacterium tuberculosis H 37 R v  and the effect of streptomycin.


Studies on mammalian hetero- -galactosidases.


Effect of hormones on renal gluconeogenesis.


Thionucleotides in the transfer ribonucleic acids of Pseudomonas aeruginosa and Escherichia coli.


Ribonucleoprotein nature of an organ-specific protein from monkey and goat brain.


The modification of the tryptophan residues of bovine  -lactalbumin with 2-hydroxy-5-nitrobenzyl bromide and with dimethyl(2-hydroxy-5-nitrobenzyl)sulphonium bromide. II. Effect on the specifier protein activity.


The myoglobin of primates. 3. Cercopithecidae (Old World monkeys): Papio anubis (olive baboon) and Macaca fascicularis (=irus, crab-eating monkey).


Changes in haemoglobin concentrations during the development of kwashiorkor.


ABO blood groups, rhesus factor and intestinal metaplasia of the stomach. The presence or absence of intestinal metaplasia of the stomach was determined in 272 patients by direct vision biopsy of standardized intragastric sites using fiberoptic gastroscopy. Analysis of ABO blood groupings and Rhesus factor failed to reveal an association with metaplasia.


Epidemiology of serum hepatitis.

Epidemiology of serum hepatitis.


[Decarboxylase activity of the brain capillary walls and parenchyma in the rat, cat and monkey].


Immunoelectrophoresis--applications and recent developments.


Peptidases and naphthylamidases in human skin. Influence of buffers, EDTA and metals on dipeptide arylamidase 3.


Serum copper levels and epilepsy.

Serum copper levels and epilepsy.


Outlook for Chagas' disease. Insecticides for short-term, improved housing for long-term.


An immunochemical study of pigeon-breeder's disease.


An immunochemical study of pigeon-breeder's disease. IV. A highly selective in vitro test for pigeon fancier's lung based on complement (C3) inactivation.


An immunochemical study of pigeon-breeder's disease. 3. The interaction of pigeon intestinal enzyme with human serum and a study of serum inhibitors.


Organ-specific and cross-reacting isoenzymes in human alkaline phosphatases.


Influence of selected synergists on the action of five insecticides on larval western spruce budworm: absence of synergism and in vitro oxidation of Zectran.


Control of green peach aphid on sweet peppers in Delaware.


Chemical control of coneworms.

Chemical control of coneworms.


Pecan spittlebug: chemical-control studies.


The LH-releasing hormone test in 31 women with secondary amenorrhoea.


The treatment of convulsive disorders.

The treatment of convulsive disorders.


The eye and adverse drug effects. Some selected considerations.


Gross hyperkeratosis of palms and soles, pemphigoid and epilepsy.


Complications of the cross-leg flap operation.


A quarter century of intracaval feeding.


Inherited blood clotting disorders. Report of a WHO scientific group.


Education and training for family planning in health services. Report of a WHO study group.


Absorption of pteroylglutamate and dietary folates in man.


Experimentally induced sandfly fever and vitamin metabolism in man.


Interrelated changes in host metabolism during generalized infectious illness.


Characterization of the ribosomal ribonucleic acids of blue-green algae.


The effect of ethylenediaminetetraacetate and its calcium chelate on the rate of swelling of a strain of Pseudomonas aeruginosa in salt solutions.


Utilization of single L-amino acids as sole source of carbon and nitrogen by bacteria.


Studies in intermicrobial symbiosis. Saccharomyces cerevisiae and Lactobacillus casei.


Some characteristics of fluorescent pseudomonads isolated from surface waters and capable of growth at 41C.


Influences of environmental ionic strength, pH, and magnesium ion on bactericidal and lytic activities of  -lysin on Bacillus subtilis.


The study of biological macromolecules using perturbed angular correlations of gamma radiation.


Crystal structure of human erythrocyte carbonic anhydrase C. VI. The three-dimensional structure at high resolution in relation to other mammalian carbonic anhydrases.


Oral metolazone: effects on urine composition in water-loaded normal man.


Benorylate and gastrointestinal blood loss.


A study of the local reaction to intramuscular disodium carbenicillin (Geopen).


Reserpine, hydralazine, hydrochlorothiazide combination (Ser-AP-ES) in essential hypertension.


Low dosage phenothiazine therapy: effective anxiolytic action without impairment to intellectual function.


Fluspirilene in the treatment of chronic schizophrenic outpatients.


Clinical evaluation of the efficacy of molindone and chlordiazepoxide in anxious outpatients.


Pilot study of growth hormone release in sleep of the chair-adapted baboon: potential as model of human sleep release.


Intravenous doxycycline (Vibramycin I.V.): a clinical evaluation.


The refractory period following estrogen-induced LH surges in the rhesus monkey.


Effects of synthetic TRH on in vitro and in vivo prolactin release in the rat.


The effect of metformin on the absorption of vitamin B 12 .


Dirofilaria (Nochtiella) linstowi n.sp., a filarioid of monkeys in Ceylon.


Studies on the properdin system: isolation of a heat-labile factor from guinea pig serum related to a human glycine rich beta-glycoprotein (GBG or factor B).


The release of granule enzymes from human neutrophils stimulated by aggregated immunoglobulins of different classes and subclasses.


Comparative studies of vertebrate immunoglobulins.


Lactic acid efflux from ischaemic brain. An experimental study.


Bacterial ribonucleic acid in the frog brain after a bacterial peritoneal infection. Frogs were injected intraperitoneally with bacteria, and the RNA of the brains (which have protective barriers against the bacteria used) was extracted. Part of the RNA was bacterial RNA apparently resulting from the transcription of DNA transferred from bacteria to the brain cells.


Leukaemia and allied disorders. 3. Clinical findings in 200 personal cases.


Plasma testosterone levels in the male rhesus: influences of sexual and social stimuli. Four adult male rhesus monkeys were provided access individually to a group of receptive females. Each male assumed dominance and engaged in frequent copulations. Plasma testosterone levels increased two- to threefold during this period. Next, each male was subjected to sudden and decisive defeat by a large all-male group, and plasma testosterone fell following this experience. Two males were later reintroduced to the females, and plasma testosterone rose rapidly to the previous elevated levels.


Clinical puerperal paresis and hypocalcaemia in cattle. A statistical and genetic investigation.


Congenital afibrinogenemia in goats.

Congenital afibrinogenemia in goats.


Studes on the extragonadal reserves of spermatozoa (vasa deferentia and ampullae) in water buffalo bulls.


Effects of oestrogens on thyroxine turnover in hyperthyroidism.


Peripheral plasma levels of oestrogens, progesterone and 17 -hydroxyprogesterone during the menstrual cycle of the rhesus monkey.


Australia antigen and antibody in transfused children with thalassaemia.


Screening for cystic fibrosis.

Screening for cystic fibrosis.


Molecular composition and sedimentation characteristics of soluble antigen-antibody complexes.


Thermodynamic studies of transfer ribonucleic acids. II. Characterization of the thermal unfolding of yeast phenylalanine-specific transfer ribonucleic acid.


Spontaneous diabetes in Macaca nigra.

Spontaneous diabetes in Macaca nigra.


Some properties of ATP dependent deoxyribonucleases from normal and rec-mutant strains of Bacillus subtilis.


Gold excretion correlated with clinical course during chrysotherapy in rheumatoid arthritis.


Transport of nuclear DNA into the cytoplasm in cultured animal cells. A survey.


Effects of high concentrations of salt on the esterase activity of human carbonic anhydrase.


The quantitation of rheumatoid factors.

The quantitation of rheumatoid factors.


Kinetics of aurothiomalate in serum and synovial fluid.


Hyperprotease-producing mutants of Bacillus subtilis. A number of mutants of Bacillus subtilis producing high levels of extracellular protease have been isolated. Analysis of culture supernatants of these mutants has shown that the total amount of proteolytic activity is elevated from 16- to 37-fold over the wild strain. The elevated activity was due to a simultaneous increase in both the neutral and alkaline protease. All of the mutants genetically analyzed were found linked to the argC4 marker by PBS-1 transduction analysis.


Incompatibility groups and the classification of fi -  resistance factors. Incompatibility between R factors has been reported by several authors, and four incompatibility groups have already been described by Datta and Hedges among Rfi(-) factors. The stability of 12 plasmids in pairs was studied after 116 crosses, and five new groups were found, designated 5, 6, 7, 8, and 9. Each plasmid studied belongs to one single group. Incompatibility between plasmids in pairs is a clear-cut phenomenon, is easy to observe, and can provide a reliable method for recognizing and classifying resistance factors, and for tracing their spread among bacterial species.


Properties of RP4, an R factor which originated in Pseudomonas aeruginosa S8. RP4, an R factor derived from Pseudomonas aeruginosa S8 and specifying resistance to carbenicillin, neomycin, kanamycin, and tetracycline, could be isolated as an extrachromosomal satellite of covalently closed circular DNA (CCC-DNA) of molecular weight about 62 million and of buoyant density 1.719 g/cm(3) (60% guanine plus cytosine). When RP4 was compared by hybridization with RP1, an R factor originating in another strain of P. aeruginosa, it was shown that RP4 contains most, if not all, of the base sequences of RP1, and also additional sequences not found in RP1.


Genetic basis of the biodegradation of salicylate in Pseudomonas. The genetic basis of the biodegradation of salicylate in Pseudomonas putida R1 has been studied. This strain utilizes the meta pathway for oxidizing salicylate through formation of catechol and 2-hydroxymuconic semialdehyde. The enzymes of the meta pathway are induced by salicylate but not by catechol, and the genes specifying these enzymes are clustered. The gene cluster can be eliminated from some salicylate-positive cells by treatment with mitomycin C and appears to exist inside the cell as an extrachromosomal element. This extrachromosomal gene cluster, termed the SAL plasmid, can be transferred by conjugation from P. putida R1 to a variety of other Pseudomonas species.


5-Bromouracil-tolerant mutants of Bacillus subtilis. 5-Bromouracil (BU)-tolerant mutants of Bacillus subtilis 23 (thy his) have been isolated. Several classes of tolerant mutants were obtained by a sequential selection procedure. The classes can be distinguished by their relative BU tolerance as well as several other phenotypic characteristics. The mutants can grow for an extended period of time in minimal medium supplemented with amino acids and BU, in which the sensitive parental strain (Bu(+)) undergoes rapid cell death. Both mutants But-1 and But-1310 have a greater rate of deoxyribonucleic acid (DNA) synthesis by a factor of two in the presence of BU than Bu(+), But-1 being somewhat faster than But-1310. The preferential incorporation of thymine to BU of But-1 is about half that of the Bu(+) strain during DNA replication in minimal medium supplemented with 10 mug of BU/ml and 1 mug of thymine/ml. It is not known at what step or steps this reduction in selectivity occurs.


Genetic regulation of cell division initiation in Bacillus subtilis. The growth and division properties of a temperature-sensitive mutant of Bacillus subtilis defective in the initiation of cell division have been studied. Log-phase cells transferred from 30 to 45 C continue to increase in length but fail to initiate new divisions. Deoxyribonucleic acid synthesis continues at 45 C, and genomes are segregated along the filament length. When filaments are returned to 30 C, division initiation resumes, and the long multinucleate clones are partitioned into normal-size cells. Occasionally, multiple cross walls initiate in close proximity, resulting in tiny cells, some of which are anucleate. Division resumption is sensitive to protein synthesis inhibitors, suggesting there is a new protein required for the initiation of division in filaments.


Extrinsic cytological determinants of basket and stellate cell dendritic pattern in the cerebellar molecular layer.


Central venous nutrient--CVN.

Central venous nutrient--CVN.


Does malnutrition in early life lead to permanent brain damage?


Continuous nasogastric infusion versus total intravenous alimentation.


Essential fatty acid deficiency in an infant receiving prolonged parenteral alimentation.


Thyroid function assays in infants.

Thyroid function assays in infants.


Widespread brain damage in alcoholics.

Widespread brain damage in alcoholics.


Urinary excretion of delta aminolevulinic acid after discontinuance of therapy for lead poisoning. A test for adequacy of therapy.


The superior colliculus of the brain.

The superior colliculus of the brain.


Visual receptive fields sensitive to absolute and relative motion during tracking. Some neurons in the visual cortex of awake monkeys visually tracking a moving target showed receptive fields that were excited only by stimulus motion relative to a background, while other neurons responded to any kind of stimulus motion. This result was found with two methods, one in which tracking eye movements were identical in both relative-motion and absolute-motion conditions, and another in which stimulus motions on the retina were identical in both conditions. This response pattern can differentiate translation of the retinal image during eye movement from motion of objects in the world.


Surface studies of acute radiation injury in the mouse intestine.


Progressive muscular dystrophy of insect dorsolongitudinal muscle. Immunological and electron microscopical investigations in crickets.


The role of acrocentric chromosomes in nucleolar organization. I. Correlation between the loss of acrocentric chromosomes and a decrease in the number of nucleoli in meningioma cell cultures.


Cell proliferation in rat skeletal muscle during early stages of compensatory hypertrophy.


An electronhistochemical study on the distribution of sodium ions in the juxtaglomerular apparatus.


Ultrastructural studies on the behaviour of macrophages in the course of X-ray therapy of human skin cancer.


Studies on metachromasia. 1. The non-specificity of green metachromasia to toluidine blue.


Feulgen-DNA and fast-green histone estimations in individual cell nuclei of the cerebellum of young and old rats.


Steatotic degeneration of rat adrenocortical cells after treatment with aminoglutethimide.


Scanning electron microscopic studies of the corneal endothelium in man and monkeys.


Parasitic infection of animals in the University of Ibadan Zoo.


Soluble serum antigens of P. falciparum in Nigerians. I. Local incidence of malarial soluble serum antigens and antibodies.


Soluble serum antigens of P. falciparum in nigerians. II. Immunochemical studies.


Development and evaluation of a simple latex agglutination test for diagnosis of tuberculosis. A simple latex agglutination test (SLAT) based on modifications of existing serodiagnostic techniques, in which commercially available reagents are used, was developed for detection of antibodies against Mycobacterium tuberculosis. Tests performed on 553 serum samples from 316 individuals, including 117 bacteriologically confirmed active tuberculosis patients, showed 80% positive titers. Sera from 12 patients with arrested tuberculosis showed 91% positive titers. Nonspecific reactions were noted in 5% of 160 serums from selected normal individuals and patients with diseases other than tuberculosis. The antibodies detected by the SLAT method were found to be relatively stable when exposed to low temperatures, whereas high temperatures reduced the antibody titer considerably. Disodium ethylenediaminetetraacetic acid inactivation of serum complement was found to be satisfactory. No variation of tuberculosis antibody titer was noted in tests on multiple specimens from patients whose conditions were stabilized. However, considerable fluctuation was encountered in antibody titers obtained on recently detected individuals. Data obtained in this study indicate that the modified procedures of the SLAT method could replace the tuberculin skin test for simple screening of tuberculosis in adults.


Effect of shaking speed on the secretion of enterotoxin B by Staphylococcus aureus. The concentration of enterotoxin B secreted by four strains of Staphylococcus aureus was dependent upon the shaking speed. For the conditions established, each strain demonstrated an optimal shaking speed, and speeds in excess of the optimum resulted in decreased secretion of toxin. At the optimal shaking speed, maximum secretion occurred at 37 C. At 45 C, both growth and toxin secretion were absent. By using agar containing antienterotoxin B sera, studies with strain S-6 at optimal and suboptimal shaking speeds demonstrated that individual cells vary in their toxin-synthesizing ability and that the relative numbers of high and low producers change during the growth cycle. Although most of the toxin was secreted during the first 12 hr of growth, a portion was secreted during the subsequent 6 hr, even though growth as measured by colony-forming units per milliliter decreased and Klett units increased.


Introduction of Pseudomonas aeruginosa into a hospital via vegetables. Pseudomonas aeruginosa was isolated from tomatoes, radishes, celery, carrots, endive, cabbage, cucumbers, onions, and lettuce obtained from the kitchen of a general hospital, with tomatoes yielding both highest frequencies of isolation and highest counts. Presence of P. aeruginosa on the hands of kitchen personnel and cutting boards and knives which they used suggests acquisition of the organism through contact with these vegetables. It is estimated that a patient consuming an average portion of tomato salad might ingest as many as 5 x 10(3) colony-forming units of P. aeruginosa. Pyocine types of P. aeruginosa isolated from clinical specimens were frequently identical to those recovered from vegetables, thus implicating tomatoes and other vegetables as an important source and vehicle by which P. aeruginosa colonizes the intestinal tract of patients.


Enterococci in insects.

Enterococci in insects. Enterococci were obtained from 213 of 403 insects cultured during a 14-month period, in numbers from 10(3) to 3 x 10(7)/g of insect. Insects were taken only from nonurban, wild, and cultivated fields and woods. In species of insects carrying them, enterococci were not always present in every individual cultured, and often more than one species of enterococcus occurred within a species. Enterococci were obtained from certain insects taken in the field during the dormant season, suggesting their role as overwintering agents. They were generally present in species feeding on nectar, succulent plant parts, and on and ir forest litter, but not from insects feeding on less succulent leaves and stems. Streptococcus faecalis was recovered from 32%, Streptococcus faecium from 22.4%, and Streptococcus faecium var. casseliflavus from 43.5% of members of the 37 taxa of insects. S. faecalis and S. faecium var. casseliflavus exhibit a high percent of conformity to the properties published for them. The heterogeneity in properties of S. faecium is similar to that found for the species taken from plants. Many fail to grow in broth at 45 C or in broth containing 6.5% NaCl; 50% of the cultures ferment both melezitose and melibiose, and a few ferment neither sugar. The remainder ferment melibiose only. Failure to reduce methylene blue in milk by S. faecalis and S. faecium is correlated with the inability to ferment lactose. More than 93% of the cultures of S. faecalis digest casein in milk from the top downward, following the production of a soft, flowing curd. Because this property is not characteristic of S. faecalis taken from humans, the reaction in litmus milk is suggested as a means of differentiation between cultures of remote and innocent origin in nature and recent, human pollution.


Cellular fatty acid composition of selected Pseudomonas species. The cellular fatty acid composition of 10 reference strains representing eight species of Pseudomonas was determined by gas-liquid chromatography. A variety of acids were detected in these organisms, including branched and straight-chained acids, cyclopropane, and hydroxy acids. Comparison of the presence and relative amounts of these acids among strains was useful for distinguishing various Pseudomonas species.


Isolation of Vi antigen and a simple method for its measurement. Vi antigen was purified from saline extract of Citrobacter strain 5396/38 by enzymatic digestion, concentration with ethanol, and precipitation with hexadecyltrimethylammonium bromide. The Vi antigen prepared by this method was 18 and 230 times more protective in mice than two other preparations isolated by an earlier method utilizing acid hydrolysis. A sensitive and specific method of electroimmunodiffusion was described for measurement of Vi antigen.


Survival of human dental plaque flora in various transport media. Dental plaque samples from (i) subjects with no apparent oral disease, (ii) mentally retarded subjects with periodontal disease, and (iii) subjects with active caries were collected in three transport media viz. a dithiothreitol poised balanced mineral salt solution designated as reduced transport fluid (RTF), VMG II, and modified Stuart medium (SBL). The samples were dispersed by sonic treatment, diluted in the respective medium in which they were collected, and cultured on MM10 sucrose agar. The efficiency of the transport media in the survival of dental plaque flora was determined by comparing the quantitative recovery (expressed as percentage of the initial viable count) from the specimens stored for various lengths of time. The data showed a great variation in the recovery of the oral bacterial flora from the plaque samples. VMG II and SBL served better than RTF as storage media for non-disease-associated dental plaque cultured under strict anaerobic conditions. Recoveries of bacteria from periodontal plaque specimens stored in RTF were higher than SBL and VMG II under identical conditions. The organisms present in the carious plaque samples appeared to survive much better in RTF and VMG II than in SBL as determined by conventional anaerobic culturing technique. However, VMG II showed a higher recovery of organisms from these specimens with an increase in the storage period, suggesting multiplication of the plaque flora. RTF did not allow the growth of oral bacterial flora under all experimental conditions. On the basis of the relative performance of these media it is suggested that RTF is a satisfactory medium for the transport of oral bacteria present in the samples.


Stability of a modified, live panleucopenia virus stored in liquid phase. It has been shown that a modified, live panleucopenia virus is quite stable when stored in the medium in which it was produced.


Microbial metabolites with insecticidal properties. A screen of fungi for insecticidal activity revealed the ability of Aspergillus versicolor to make versimide, methyl-alpha-(methylsuccinimido)acrylate, a novel contact insecticide. The larvicidal activities of Alternaria tenuis and Fusarium lateritium were found to be due to tenuazonic acid and diacetoxyscirpenol, respectively. Thiolutin, cycloheximide, rubratoxin, patulin, trichothecin, an actinomycin, and scirpene-producing fungi also had insecticidal activity.


Comparison of streptococcal R antigens.

Comparison of streptococcal R antigens. At least four distinct nonspecific protein R antigens were found in streptococci of groups A, B, and C by immunodiffusion in agar gel with anti-R sera.


The biologic effect from long-term exposure of primates to carbon monoxide.


Studies on crystalline yeast phosphoglucomutase: the presence of intrinsic zinc.


Australia antigen in active chronic hepatitis in Australia: results in 130 patients from three centres.


An exo- -N-acetylglucosaminidase from Bacillus subtilis B; extraction and purification.


Reduction of anxiety in the coronary-care unit. A controlled double-blind comparison of chlordiazepoxide and amobarbital.


Amoxapine: a clinical evaluation in depressive syndromes.


ORF-8063: an early evaluation of a new antianxiety agent.


Cephaloridine prophylaxis in open heart surgery.


Oximinoacids in the inorganic nitrogen metabolism.


Sequential appearance of  ,  , and  -crystallins in embryonic and metamorphic Rana pipiens.


Prophylaxis of chemically induced papillomas and carcinomas of mouse skin by vitamin A-acid.


Factors influencing human gallstone dissolution in monkey, dog, and human bile.


Immunoglobulin measurements in jejunal secretions of patients with adult coeliac disease and dermatitis herpetiformis.


H 2 O 2  and OH radicals in radiation inactivation of bacterial spores.


On the nature of the radiation damage in the thymine containing strand of hybrid BU-DNA after long wave-length U.V.


The purification and binding properties of a human thyroxine-binding prealbumin.


Whole-body imaging and count profiling with a modified Anger camera. I. Principles and application.


Whole-body imaging and count profiling with a modified Anger camera. II. Implementation evaluation.


Diagnostic and therapeutic implications of long-term radioisotope scanning in the management of thyroid cancer.


99m Tc-EHDP: a potential radiopharmaceutical for skeletal imaging.


Isolated perfused resistance vessels and myogenic tone.


Studies on plasma renin activity in patients with renovascular hypertension.


Uptake of esters of p-hydroxybenzoic acid by Serratia marcescens and by fattened and non-fattened cells of Bacillus subtilis.


The nature of the phenolase enzyme in Mycobacterium leprae: structure-activity relationships of substrates and comparison with other copper proteins and enzymes.


Malnutrition and mental deficiency.

Malnutrition and mental deficiency.


Cardiopulmonary autotransplantation in primates (baboons): late functional results.


Unilateral "oligoméganéphronie" with agenesis of the contralateral kidney. Studied by microdissection.


Cytoplasmic tubular arrays in latent chronic glomerulonephritis.


The cloverleaf skull syndrome. Histological, histochemical and ultrastructural findings.


Morphological characteristics of the columnar epithelium lining the lower oesophagus in patients with Barrett's syndrome.


The indirect fluorescent antibody test for experimental East Coast Fever (Theileria parva infection of cattle). Detection of antibodies to schizonts in dried blood samples.


Eye muscle motor neurons with different functional characteristics.


Avian haemoproteidae. I. Description of Haemoproteus fallisi n. sp. and a review of the haemoproteids of the family Turdidae.


Toxicology of environmental agents: a blend of applied and basic research.


Mycoviruses: a new dimension in microbiology.


Use and replaceability of polychlorinated biphenyls.


Environmental factors controlling enzymatic activity in microbodies and mitochondria of Euglena gracilis.


Combined effects of noise and ototoxic drugs.


A compact form of double-stranded DNA in solution.


Energy transfer in an ANS-phycocyanin complex.


Solubilization and partial characterization of the phosphorylated component(s) of the membrane of adrenal chromaffin granules.


Effect of different serologic transformations on T 5  universes.


Purification of bacterial endotoxins by zonal centrifugation. In this paper, we describe a cultivation procedure in large-capacity fermentors (200 liters) and the purification of the Salmonella crude extracts by means of zonal centrifugation in a sucrose gradient. Briefly, the endotoxin has been extracted from salmonellae with hypertonic solutions. The crude endotoxin was shown to contain several antigens, mainly r(1), r(2), r(3), or heterologous antigen. The heavy endotoxin was isolated and purified previously by enzymatic digestion, followed by a series of gel filtrations on Sephadex or Sepharose columns. We report the isolation and purification of heavy endotoxin in sucrose gradients, with the use of a B XIV titanium zonal rotor. From 150 to 300 mg of the crude material resuspended in a solution containing 1 m sodium chloride, 0.1 m sodium citrate, and 2.5% (w/w) sucrose, has been submitted to zonal centrifugation in gradients consisting of 5 to 25% (w/w) sucrose, also containing 1 m sodium chloride and 0.1 m sodium citrate. An overlay of 200 ml of 1 m sodium chloride-0.1 m sodium citrate was introduced after the sample. The separations were obtained after centrifugation for 3.5 hr at 35,000 rev/min (integralw(2)dt = 1.66 x 10(11)) at 4 C; the heavy endotoxin sedimented as heterogeneous material, from the middle toward the distal portions of the gradient. The heterologous antigens (r(1), r(2), r(3)), as well as bacterial proteins, remained in the sample zone. The heavy endotoxin recovered from the gradients was quite pure, as revealed by immunodiffusion tests against several antibacterial sera.


Serological investigations of a bovine respiratory disease ("urner pneumonie") resembling farmer's lung. The immunological response of cattle exposed to moldy hay was examined by agar gel diffusion with standard farmer's lung hay antigens. A high incidence of precipitins against Micropolyspora faeni (60%) and moldy hay antigen (80%) was detected in exposed but apparently healthy cattle from a region with a high incidence of bovine farmer's lung. In comparison, in the plains, a low incidence area, we found only 1 animal of 164 harboring precipitins against M. faeni. We further observed that many animals from exposed populations lost their precipitins during pasturing and regained them during winter housing. Thirty-nine clinical cases of bovine farmer's lung ("Urner Pneumonie") were investigated serologically. Only 49% of these animals showed precipitins against M. faeni and 54% showed precipitins against moldy hay antigen. We discuss in this paper the probable causes of this apparent lack of immunological response.


Metabolism of Rickettsia typhi and Rickettsia akari in irradiated L cells. L cells that had been exposed to 3,000 r of (60)Co the previous day were used to study the growth and metabolism of Rickettsia typhi and R. akari. Viable (unirradiated) L cells were used to study the effect of rickettsial infection on host-cell metabolism. Monolayers were infected with a rickettsial multiplicity of 1.2 and given Eagle's minimal essential medium containing 25 mmN-2-hydroxyethylpiperazine-N'-2'-ethanesulfonic acid buffer and 10% calf serum. At various intervals, cycloheximide (2 mug/ml) was added to one set of cultures, to inhibit eukaryotic protein and deoxyribonucleic acid (DNA) metabolism; phosphate-buffered saline (PBS) was added to another set. After 1 hr, the cultures received a mixture of 15 (14)C-labeled amino acids or adenine-8-(14)C. The cultures were harvested 16 hr later and were tested for incorporation of labeled carbon into the fraction precipitated by cold trichloroacetic acid. Viable cells were exposed to thymidine-2-(14)C for 2-hr periods. Infectivity of R. typhi increased to a peak of 150 to 400 hemolytic units/culture on day 4; the titer remained approximately the same on days 5 and 6, and declined rapidly on day 7. Total amino acid incorporation was about the same in infected and uninfected cultures up to day 6, but metabolic activity was reduced to a negligible level on day 7 in infected cells. Cycloheximide-resistant activity was higher in the infected cultures, with a peak equivalent to one-half the total activity at day 4 to 5. Total as well as cycloheximide-resistant adenine incorporation was higher in the infected cells between days 3 and 5 after infection, with a peak at day 3 to 4. Somewhat similar results were obtained with R. akari, except that the cycle of infection and of cycloheximide-resistant activity proceeded and was completed more rapidly. (14)C-DNA of both rickettsiae was isolated from infected cultures that had received labeled adenine. With labeled thymidine, which was not incorporated by the rickettsiae, it was shown that R. typhi and R. akari differ considerably in their effects on the host cell. R. typhi elicited moderate inhibition, whereas R. akari infection led to a complete inhibition of thymidine incorporation by the third day, at the time of highest rickettsial activity. It is concluded that rickettsiae have the necessary enzymes for protein and nucleic acid synthesis, but, thus far, these enzymes have been activated or induced only in an intracellular environment.


A goitrous subject with structural abnormality of thyroglobulin.


Thyrotropin releasing factor (TRF) effect on secretion of human pituitary prolactin and thyrotropin in children and in idiopathic hypopituitary dwarfism: further evidence for hypophysiotropic hormone deficiencies.


Growth hormone levels in protein calorie malnutrition.


An absorption method for preparing anti-M-typing streptococcal sera using acid-hydrolyzed cells.


Organized epithelioid cell granulomate elicited by schistosome eggs in experimental animals.


An information center's place in military medicine.


On the lymphatic vessels in the stroma of the cornea.


Teratogenicity of adenine in the rat embryo.


Autonomy or nonautonomy of gene effects in mosaics. The dominant autosomal gene Msc (Multiple sex combs) of Drosophila causes the appearance of sex combs on the second and third legs and a reduction of size of sex combs on first legs. The effect of Msc was studied in mosaic flies in which non-Msc spots were present on a Msc background. Mosaics were due to somatic crossing-over induced by x-rays. Analysis of mosaics suggested autonomy of the action of Msc, provided that the somatic crossing-over occurs less frequently proximally to the Msc locus than distally. This unusual ratio of somatic crossing-over was confirmed by a separate experiment with the gene Kinked, whose locus is very close to Msc.


In vitro repair of x-irradiated DNA extracted from Bacillus subtilis deficient in polymerase I. DNA extracted from x-irradiated cells of a mutant of Bacillus subtilis deficient in DNA polymerase I has greatly reduced biological activity. This DNA, which has many single-strand nicks, can be repaired in vitro with purified DNA polymerase I and DNA ligase or with lysates of wild-type B. subtilis cells, which restore biological activity and increase the single-strand molecular weight. Lysates of polymerase I-deficient B. subtilis cells cannot repair such irradiated DNA until purified DNA polymerase I or lysate from wild-type cells is added to the deficient lysate.