Canine lymphocytes that form rosettes with human red blood cells.


Effect of hypertonic mannitol on ischemic kidneys.


In memoriam Stephen Rusznyak (1886-1974).


Preparation and properties of adenylate cyclase from Escherichia coli.


Reversible inhibition of respiration of Escherichia coli by hyperoxia.


Application of methyl methacrylate (Kallocryl M) in morbid anatomy.


Editorial: Treatment of renal failure in Australia.


Australian National Renal Transplantation Survey. Third report by a subcommittee.


Australian maintenance dialysis survey.

Australian maintenance dialysis survey.


A controlled clinical trial of a new antianxiety agent lorazepam (Ativan).


George Joseph Duncan.

George Joseph Duncan.


Henry George Douglas Cookson.

Henry George Douglas Cookson.


Experiences with malaria in the early phase of Australian army involvement in South Vietnam.


Keith Ferguson Brady.

Keith Ferguson Brady.


IgE globulins and immunity.

IgE globulins and immunity.


William Glover Howie Epps.

William Glover Howie Epps.


Joseph Harry Coles.

Joseph Harry Coles.


Robert Hargreaves Orr.

Robert Hargreaves Orr.


Letter: The use of Microstix in the diagnosis of urinary tract infections.


A century of anatomy in South Australia.


Carbenoxolone sodium in the treatment of gastric ulceration in Chinese.


A comparison of flurbiprofen and aspirin in the treatment of rheumatoid arthritis.


Donald Edmund Copping.

Donald Edmund Copping.


C. V. Crockett.

C. V. Crockett.


Nucleotide sequence of the 3' terminus of E. coli 16S ribosomal RNA.


Difference in susceptibility to restriction by E. coli B between various heteroduplex molecules of bacteriophage fd DNA.


Formyl-Met-tRNA f Met, a questionable initiator tRNA in eukaryotic systems.


The enzymatic binding of aminoacyl-tRNA to reticulocyte ribosomes: the stimulatory effect of donor site bound peptidyl-tRNA.


Obituary: Dr. Ram Ginde.

Obituary: Dr. Ram Ginde.


Lesions of experimental swine atrophic rhinitis and Alcaligenes bronchisepticus antigen detected by fluorescent antibody technique.


A history of our health services.

A history of our health services.


A beveled angular scleral incision for foreign body extraction.


Mucous membrane grafting for cicatricial entropion.


Provocative tests in angle-closure glaucoma: a brief review with commentary.


Tear gas keratopathy in a child: treatment by keratoplasty.


Primary and secondary reconstruction of the eyeball after extensive lacerations.


Entropion of lower lid: repair by double triangle tarsectomy.


Free tissue patch and cyanoacrylate in corneal perforations.


Modified enucleation procedure.

Modified enucleation procedure.


A comparison of dantrolene sodium and diazepam in the treatment of spasticity.


Editorial: How Pathologica Europaea was born.


Hexagonal crystalline bodies of IgA in the cytoplasm of the hepatocytes.


On the hospitalization of children: an historical approach.


Letter: Comment on meningomyelocele treatment.


Selection for early treatment in myelomeningocele: a retrospective analysis of various selection procedures.


Baccalaureate education in nursing: key to professional career in nursing 1974-75.


Letter: Haloperidol in stuttering.

Letter: Haloperidol in stuttering.


The clinical and hormonal effect of luteinizing hormone-releasing hormone (LH-RH) in cows with cystic ovaries.


Pathophysiological findings in fascioliasis.


Immunity in bovine fascioliasis.

Immunity in bovine fascioliasis.


A new simpler photoaffinity analogue of peptidyl tRNA. The synthesis of the n-hydroxysuccinimide ester of N-(2-nitro-4-azidophenyl)glycine (NAG) is described. This reacts with E. coli phe-tRNA(Phe) to yield the photoaffinity label NAG-Phe-tRNA(Phe). This peptidyl tRNA analogue binds correctly to the peptidyl site of the E. coli ribosome. The only significant covalent products found after irradiation of a peptidyl site bound NAG-Phe-tRNA(Phe)-70S-poly(U) complex are 50S proteins L11 and L18. After irradiation the complex can still bind [(3)H]Phe-tRNA to the amino acyl site and participate in peptide bond formation with the covalently attached NAG-Phe moiety. Alternatively, one can allow peptide bond formation to occur first, prior to photolysis. The reaction products are still L11 and L18. Hence, both of these two proteins appear to be centrally located at the peptidyl transferase center.


Replication of colicinogenic factor E 1 DNA: evidence for a discontinuous replication mechanism. The mechanism of Col E 1 DNA replication was investigated in a plasmolysed cell system prepared from chloramphenicoltreated E. coli JC 411 (Col E 1). After pulse-labelling with (3)H-dTTP a considerable fraction of the newly synthesized DNA was recovered as single-stranded fragments. Upon alkali denaturation the pulse label was found in DNA chains sedimenting slower than unit length Col E 1 strands with a prominent peak at 5 S. During a chase with unlabeled precursors the label is transferred nearly completely into supercoiled Col E 1 DNA. DNA ligase appears to be required for the joining of the 5 S pieces since in the absence of NAD an accumulation of short fragments is observed.


Immunology of leprosy.

Immunology of leprosy.


Enzymatic methylations: III. Cadaverine-induced conformational changes of E. coli tRNA fMet as evidenced by the availability of a specific adenosine and a specific cytidine residue for methylation. A partially purified tRNA methylase fraction from rat liver, containing m(2)G- m(1)A- and m(5)C-methylase, was used to study the influence of Mg(++) and of the biogenic polyamine cadaverine on the enzymatic methylation of E.coli tRNA(fMet)in vitro. In presence of 1 or 10 mM Mg(++), guanosine no. 27 was methylated to m(2)G. In 1 mM Mg(++) plus 30 mM cadaverine, guanosine in position 27 and adenosine in position 59 were methylated. In presence of 30 mM cadaverine alone tRNA(fMet) accepted three methyl groups: in addition to guanosine no. 27 and adenosine no. 59 cytidine no. 49 was methylated. In order to correlate tRNA(fMet) tertiary structure changes with the methylation patterns, differentiated melting curves of tRNA(fMet) were measured under the methylation conditions. It was shown that the thermodynamic stability of tRNA(fMet) tertiary structure is different in presence of Mg(++), or Mg(++) plus cadaverine, or cadaverine alone. From the differentiated melting curves and from the methylation experiments one can conclude that at 37 degrees in the presence of Mg(++) tRNA(fMet) has a compact structure with the extra loop and the TpsiC-loop protected by tertiary structure interactions. In Mg(++) plus cadaverine, the TpsiC-loop is available, while the extra loop is yet engaged in teritary structure (G-15: C-49) interactions. In cadaverine alone, the TpsiC-loop and the extra loop are free; hence under these conditions the open tRNA(fMet) clover leaf may be the substrate for methylation. In general, cadaverine destabilizes tRNA tertiary structure in the presence of Mg(++), and stabilizes tRNA(fMet) tertiary structure in the absence of Mg(++). This may be explained by a competition of cadaverine with Mg(++) for specific binding sites on the tRNA. On the basis of these experiments a possible role of biogenic polyamines in vivo may be discussed: as essential components of procaryotic and eucaryotic ribosomes they may together with ribosomal factors facilitate tRNA-ribosome binding during protein biosynthesis by opening the tRNA tertiary structure, thus making the tRNA's TpsiC-loop available for interaction with the complementary sequence of the ribosomal 5S RNA.


Bioassay of prolactin.

Bioassay of prolactin.


In pursuit of medical history.

In pursuit of medical history.


Dentate lesions for involuntary movement.


Ethical problems surrounding dialysis and transplantation.


Renal transplantation.

Renal transplantation.


Anaesthetic problems of renal transplantation.


The relationship between response to phenelzine and acetylator status in depressed patients.


Sir William Macewen.

Sir William Macewen.


Respiratory distress syndrome. Continuous positive airway pressure (CPAP) using the Gregory box.


Methods of mechanical ventilation for hyaline membrane disease.


Surgical aspects of burns.

Surgical aspects of burns.


Changing views on oral disease.

Changing views on oral disease.


The nurse who is a bit larger than life (Col. Winnie W. Luzon).


This issue's personality: Purita F. Asperilla.


Complement in immunohematology.

Complement in immunohematology.


Haffkine--A historical appreciation.

Haffkine--A historical appreciation.


Some practical problems of the epidemiology of leprosy in the Indian context.


Recent progress in the chemotherapy of schistosomiasis.


Clinical applications of in vitro lymphocyte tests.


Comparative evaluation of amoebicidal drugs.


New perspectives on the chemotherapy of malaria, filariasis, and leprosy.


Fungi isolated from bovine mycotic abortion and pneumonia with special reference to Mortierella wolfii.


In vivo and in vitro studies with the T1 strain of Mycoplasma mycoides in cattle.


The effect of enteropathogenic strains of Escherichia coli and their heat-stable enterotoxin on the ligated intestine of adult pigs.


Immunity. I. Review of basic immunology.


Temperature regulation: the spinal cord as a site of extrahypothalamic thermoregulatory functions.


Hypothalamic-pituitary-gonadal function in two siblings with Prader-Willi syndrome.


Recent concepts of intestinal fat absorption.


The study on the advantages, indications and limitations of instrumental hemostasis of the hard bleeding edges of the uterine wound in caesarean sections.


Antimony, arsenic, bromine and mercury in enamel from human teeth.


Effects of cholecystokinin, secretin, 2-deoxy-D-glucose and insulin on pentagastrin-stimulated gastric secretion in fistula chickens.


Papain-induced changes in the cranial base of rats.


Platelet aggregation and haemolysis induced in rats by intravenous infusion of ADP - effect of potentially antithrombotic drugs.


Technical aspects of the rosette technique for detecting human circulating B and T lymphocytes. Normal values and some remarks on null lymphocytes.


Influence of two different beta-adrenergic blocking agents on the increase in fibrinolytic activity and factor VIII activity during submaximum and maximum exercise.


Duration of thymic function.

Duration of thymic function.


A rappraisal of bursal functions.

A rappraisal of bursal functions.


Fifty years of Soviet genetics and breeding of plants, animals, and microorganisms.


Study of repair processes in the yeast Saccharomyces cerevisiae. Communication V. Combined action of photoreactivating light and postradiation incubation in buffer on the genetic effects of ultraviolet irradiation.


Study of H-2 mutations of mice: analysis of mutation 504 by the method of F1 hybrids.


AAHE directory of institutions offering specialization in health education.


Genotypic control of the formation of complete mutants in Saccharomyces cerevisiae.


Specificity of the mutagenic action of fast neutrons.


Study of repair processes in the yeast Saccharomyces cerevisiae. Communication VI. The induction of back mutations to UV-sensitive yeasts and liquid holding recovery.


Genetic and physiological investigation of rifampicin-resistant RNA polymerase mutants of Escherichia coli K-12. Communication II. Localization of the rifampicin locus on the chromosome map and its fine mapping.


Comparative genetics of yeast. Communication X. Reidentification of mutations of mating types in Saccharomyces.


Comparison of the activities of nitrogen mustards according to induced mitotic crossing over in a diploid strain of Aspergillus nidulans.


The role of the rec A+, rec B+, and rec C+ genes of the donor Escherichia coli K-12 in the repair of rec minus recipients, irradiated with gamma rays at absolutely lethal doses.


Replication of the sexual and colicinogenic factors in the cell cycle of Escherichia coli.


Transfer of the transmissive determinants of hemolytic activity of Escherichia coli in the organism of gnotobiont chicks.


Analysis of ethylenimine-induced adenine mutants in strains of the yeast Saccharomyces cerevisiae, differing in sensitivity to ultraviolet and x-rays.


In memoriam: Herbert Minchinton Bennett.


Symplasmic transport in plants.

Symplasmic transport in plants.


Some new insights concerning cytoplasmic transport.


Dynamic activities and labile microtubules in cytoplasmic transport in the marine foraminiferan Allogromia.


Fast transport along nerves.

Fast transport along nerves.


Intra-axonal transport of neurotransmitters in mammalian neurons.


Development and fine structure of angiosperm and gymnosperm sieve tubes.


Movement of extracellular enzymes across cell membranes.


Membrane events during the secretory process.


Biochemical aspects of phloem transport.


Mechanisms of endocytosis and exocytosis.


Metabolic co-operation between cells in tissue culture.


Transport from symbiotic algae and symbiotic chloroplasts to host cells.


The mechanisms of phloem translocation.

The mechanisms of phloem translocation.


Diagnosis of thrombosis. Evaluation of 125I-fibrinogen scanning and blood tests.


Blood flow and thrombosis.

Blood flow and thrombosis. Platelet thrombi have been observed at bifurcations, constrictions and stenoses in the arterial tree where flow separation with accompanying vortex formation can occur. Hence, studies of the flow behavior of human blood cells in models of a vessel obstruction and a sudden expansion of a vessel lumen were carried out. These showed that the particles were subjected to first high, and then much lower shear stresses as they entered the vortices where the environment appeared favorable for the formation of aggregates. The effects of high and variable shear stress on the release reaction and aggregation of platelets were therefore studied using cells labeled with C14-Serotonin suspended in Tyrodes-albumin undergoing oscillatory tube flow for 20 min at 37degrees C. There was no significant release of serotonin due to flow alone, but the thrombin-induced release of C14-activity was appreciably greater in suspensions subjected to shear rates greater than 2000 sec-1 than in control suspensions which had been incubated at rest. The synergistic effects of the shear flow were also noted in the presence of adrenaline, which increased the release reaction, and when [Ca++] was raised above the physiological level or acetyl salicylic acid added, which inhibited the release of serotonin.


Platelet inhibition in the management of thrombosis. The role of platelets in the initation of arterial thrombosis is clear. In venous thrombosis as well, platelets may in some circumstances play a significant role. For these reasons, and because of the complications and limitations of anticoagulant therapy, antithrombotic trials have been launched with several agents which inhibit platelet function. Regarding postoperative deep vein thrombosis, neither aspirin nor dipyridamole alone appears effective, although the combination offers promise. Results with dextrans are conflicting. In recurrent idiopathic deep vein thrombosis, sulfinpyrazone may be beneficial. On the arterial side, transient cerebral ischemic attacks may be favorably affected by either aspirin or sulfinpyrazone. Prevention of thromboembolism associated with prosthetic heart valves appears possible with combination warfarin-dipyridamole therapy, and the beneficial effect of sulfinpyrazone on shortened platelet survival in this group suggests that this agent may also be effective. Sulfinpyrazone may also be beneficial in preventing thrombosis in arteriovenous canulas. The issue which has attracted the greatest attention and about which no clear answer exists at present is whether antiplatelet agents can modify the course of acute myocardial infarction. Several trials with aspirin are currently underway, and it would be premature to recommend its use in this condition until the results of these trials are available, probably in 1975.


Degradation products of fibrinogen and crosslinked fibrin-projected clinical applications.


4-Hydroxyestrone: a new metabolite of estradiol-17 beta from humans.


The management of acute and chronic renal failure in children.


Treatment of nephrotic syndrome.

Treatment of nephrotic syndrome.


Congenital cystic disease of kidney: classification and manifestations.


Renal transplantation. I. Use of donor organs with multiple vessels.


Renal transplantation. II. Lymphatic complications.


Renal transplantation. III. Prevention of wound infections.


Renal salvage through autotransplantation.


Thrombosis of perfused cadaver kidney.

Thrombosis of perfused cadaver kidney.


Use of methylprednisolone acetate in vasectomy.


Uroradiographic media.

Uroradiographic media.


Ecological research trends in parasitology.


The determination of vitamin A and some aspects of its distribution, mobilization and transport in health and disease.


The manifestations and management of severe protein-calorie malnutrition (kwashiorkor).


Physiological and metabolic effects of antioxidant food additives.


Nutrition, health and social factors related to intellectual performance.


Interest for the study of dietary absorption and iron fortification.


Nutritional and physiological consequences of the Maillard reaction.


The biochemistry of helminths.

The biochemistry of helminths.


Immunopathology of pyelonephritis: studies on the pathogenesis and diagnosis of the renal lesion.


Cryo-scanning electron microscopy of the lungs.


A simple equipment for vapour fixation and its application to lung biopsy.


Ultrastructural organization of endothelial cells in the capillaries of the avian pecten oculi.


Immunologic studies in bronchogenic carcinoma.


The chemistry of cyclopropano-steroids.

The chemistry of cyclopropano-steroids.


Observations upon haematophagy in Venezuelan Triatomids fed upon poikilotherms.


Approved list of schools American Association of Nurse Anesthetists December, 1974.


The bacterial flora of the conjunctival anophthalmic socket in glass prosthesis-carriers.


A comparison of the local anesthetic properties of bupivacaine and two new long-acting agents, HS 37 and etidocaine, in epidural analgesia.


Graft thickness after penetrating keratoplasty.


Heart rate variation in infants with the respiratory distress syndrome.


A comparison between diazepam and haloperidol in anxiety states.


Jerzy Konorski, 1903-1973.

Jerzy Konorski, 1903-1973.


[Scanning electron microscopy of the islets of Langerhans in ducks and chickens].


Professor Jerzy Konorski.

Professor Jerzy Konorski.


Anatomo-clinical correlations in amyloidosis of the islets of Langerhans.


Jerzy Konorski.

Jerzy Konorski.


Professor Jerzy Konorski -- my teacher.

Professor Jerzy Konorski -- my teacher.


Bibliography of professor Jerzy Konorski's papers.


Respiratory insufficiency following lung contusion: an experimental study.


Collateral circulation in renal allotransplants.


Experimental thyroiditis in guinea pigs.


Porphyria cutanea tarda. New facts on aetiology, pathogenesis clinical manifestations and treatment.


Proteases of Clostridium botulinum. VI. The role of trypsin, Clostridium botulinum proteases and protease inhibitors in the formation and activation of toxin in growing cultures of Clostridium botulinum.


Pulmonary lesions in dogs induced by intravenous injections of disintegrated cells of Escherichia coli.


The current status of lithium in psychiatry.


Appreciation of the Keith, Wagener, and Barker classification of hypertensive disease.


Some different types of essential hypertension: their course and prognosis.


The leukemic phase of histiocytic lymphoma: report of four cases.


New improvement of the melting and casting machine for cobalt-chromium casting alloy using the vacuum method.


Neurohaemal organs in insects.

Neurohaemal organs in insects.


Injury and repair in arterial tissue: experimental models: types and relevance to human vascular diseases--a survey.


Inactivation of chitin synthase in Saccharomyces cerevisiae.


The bile acids and fecal sterols.

The bile acids and fecal sterols.


Review and critique of cholesterol methodology.


The diagnosis of hemolytic anemias.

The diagnosis of hemolytic anemias.


The curative effects of cysteamine, cysteine, and dithiocarb in experimental paracetamol poisoning.


In memoriam. James B. Hartney, M.D. July 2, 1921-February 9, 1972.


Therapy of deep-seated fungal infections with 5-fluorocytosine.


An evaluation of secondary failure to tolbutamide therapy.


Comparison of direct and indirect arterial pressure measurements in hospitalized patients.


Effects of ethidium bromide on chick fibroblasts and mouse Ehrlich tumor cells cultivated in vitro. Cytological and cytochemical observations.


Effect of cooling on the local Shwartzman reaction.


Two cell lines from lymphomas of Marek's disease.


The cell-envelope glycolipids of baker's yeast. Sphingolipids were found to dominate in the glycolipids from the cell envelope of baker's yeast. A relatively large quantity of ceramides was detected. Among the several complex phosphosphingolipids described, ceramide-(P-inositol)(2)-mannose was the main component. About 55% of long-chain bases in sphingolipids consisted of C(18)-phytosphingosine (4D-hydroxysphinganine). Other bases, found in decreasing concentrations, were C(20)-phytosphingosine, C(20)-dehydrophytosphingosine, C(18)-dihydrosphingosine (sphinganine) and C(19)-dihydrosphingosine. The presence of sterol glycosides, sulpholipids, cerebrosides and acylglucoses was demonstrated.


Action of dimeric hybrids of native and selectively alkylated ribonuclease A on double-stranded ribonucleic acid. The hypothesis that both active sites of dimeric ribonuclease A have to be simultaneously available in order to make the enzyme able to degrade double-stranded RNA is tested with purified hybrid dimers of ribonuclease A and its carboxymethylated derivative. The results obtained support the hypothesis.


Kinetics of irreversible enzyme inhibition by an unstable inhibitor. A mathematical treatment for the general case of enzyme inactivation by an inhibitor that breaks down in solution in a first-order reaction is presented. Cathepsin D was inactivated by fluorescein isothiocyanate with a K(i) of 4.47mum. Kinetic constants were also determined for the inactivation of cathepsin D by 1,1-bis(diazoacetyl)-2-phenylethane, and the inactivation of pepsin C by diazoacetyl-dl-norleucine methyl ester.


Characterization of the lac repressor species produced by limited tryptic cleavage.


The primary structure of the toxin Laticauda semifasciata III, a weak and reversibly acting neurotoxin from the venom of a sea snake, Laticauda semifasciata. A weak and reversibly acting neurotoxic protein of Laticauda semifasciata venom, Laticauda semifasciata III (component LsIII), was sequenced. Component LsIII consists of 66 amino acid residues and has five disulphide bridges, one of which was located between residues 26 and 30. The weak and reversible neurotoxicity of component LsIII is discussed in relation to its structure, which falls between those of the neuro- and cardiotoxins of sea snakes and Elapidae snakes isolated and sequenced so far.


Changes in the number of binding sites for ribonucleic acid polymerase in chromatin of WI-38 fibroblasts stimulated to proliferate. 1. When WI-38 human diploid fibroblasts form confluent monolayers, DNA synthesis and cell division almost completely cease. A change of medium causes these density-inhibited cells to proliferate and within 1h after the application of the stimulus there is an increase in template activity of the chromatin isolated from stimulated cells. 2. The number of binding sites for Escherichia coli RNA polymerase was determined on chromatin from WI-38 cells by two different methods, i.e. incorporation of [(3)H]UTP into RNA in the absence of reinitiation, and incorporation of [gamma-(32)P]GTP into chain termini. 3. Both methods indicate that the capacity of chromatin to bind E. coli RNA polymerase is increased in WI-38 cells stimulated to proliferate. 4. The increase in the number of binding sites for E. coli RNA polymerase parallels the increase in chromatin template activity and suggests that the latter reflects an increase in the number of initiation sites, rather than an increase in the rate of transcription.


Yeast episome: oligomycin resistance associated with a small covalently closed non-mitochondrial circular DNA.


Common precursors of human blood group MN specificities.


Removal of fibroblastoid cells from primary monolayer cultures of rat neonatal endocrine pancreas by sodium ethylmercurithiosalicylate.


The mechanism of replication of phiX174 single-stranded DNA.  X. Distribution of the gaps in nascent phiX174 replicative form DNA.


The mechanism of action of initiation factor 3 in protein synthesis. II.  Association of the 30S ribosomal protein S12 with IF-3.


Identification of 6-O-alpha-D-mannopyranosyl myo-inositol from Saccharomyces cerevisiae.


Reaction of p-nitrophenyl trimethylacetate with yeast carboxypeptidase.  Evidence for an acyl-enzyme intermediate.


Transcriptional termination in vitro: the 3'-terminal sequence of coliphage T7 "early" RNA.


The effect of ionising radiation and chemical methylation upon the activity and accuracy of E. COLI DNA polymerase I.


Effect of sodium dodecylsulfate and heating on protein conformation in outer and cytoplasmic membranes from Escherichia coli.


The role of a novel cytochrome b-containing nitrate reductase and quinone in the in vitro reconstruction of formate-nitrate reductase activity of E. coli.


In vitro transcription of RNA tumor virus genes in the chromatin of uninfected chicken cells.


Lymphosarcoma cell growth is selectively inhibited by B lymphocyte mitogens: LPS, dextran sulfate and PPD.


Nalidixic acid-sensitive and resistant modes of DNA replication in Escherichia coli.


Enzymatic mechanisms for the inactivation of luteinizing hormone-releasing hormone (LH-RH).


Obituary: John Anthony Francis Xavier Bell, L.D.S.


Effects of bone grafting on orthodontic tooth movement in children with cleft lip and palate.


Fissure sealants. Further results of clinical trials.


New hand instruments for subgingival scaling.


British society of periodontology.

British society of periodontology.


Thomas Howard Dunseith, L.D.S.

Thomas Howard Dunseith, L.D.S.


Jacques Kurer, M.D., L.D.S.

Jacques Kurer, M.D., L.D.S.


Carl Robert Rudolf, L.D.S.

Carl Robert Rudolf, L.D.S.


Editorial: Centennial of the Hospital of the University of Chile (1874-1974).


Walter Ernest Coe, D.M.D., L.D.S.

Walter Ernest Coe, D.M.D., L.D.S.


Neutrophil function in chronic granulocytic leukaemia before and after busulphan treatment.


Diet and abnormal behaviour: views of a pioneer medical psychologist.


An immunological study of complementary fragments of beta-galactosidase.


Strand and site specificity of the relaxation event for the relaxation complex of the antibiotic resistance plasmid R6K.


Isolation and in vitro restriction of unmethylated DNA from Escherichia coli K.


Sequential biochemical events related to DNA replication in the regenerating rat liver.


The effects of lysozyme on DNA--membrane association in Escherichia coli.


Purification on hydroxyapatite of liver ribosomes and polysomes from unfasted mice.


Blood dimethyltryptamine concentrations in psychotic disorders.


High molecular weight polypeptides synthesized using DNA as messenger.


Pituitary responsiveness to luteinizing hormone-releasing hormone during delayed implantation in the spotted skunk (Spilogale putorius latifrons): validation of LH-radioimmunoassay.


Rapid movements of the chromatoid body in living early spermatids of the rat.


Uterine luminal epithelium: protrusions mediate endocytosis, not apocrine secretion, in the rat.


Changes in testis specific lactate dehydrogenase isoenzymes during the seasonal spermatogenic cycle of the marsupial Schoinobates volans (petauridae).


Sexual dimorphism in mammals.

Sexual dimorphism in mammals.


Rheotropism in fishes.

Rheotropism in fishes.


Analysis of various parameters in the technique of the rheumatoid rosette test (attemped standardization).


Effect of oral administration of monosodium glutamate (MSG) on the hypothalamic arcuate region of rat and mouse: a histological assay.


Computer diagnosis in the class of compatible diseases with reference to tumors and tumorlike formations of the female internal genitalia.


Pancreatic insulin content in normal and decapitated fetal rats.


Forensic psychiatry--a specialty.

Forensic psychiatry--a specialty.


Isaac Ray: have we learned his lessons?

Isaac Ray: have we learned his lessons?


Dangerousness as a criterion for confinement.


The Lionel Penrose memorial meeting. A biographical note.


Penrose's work and views in relation to anthropology.


The statoconia of the non-mammalian species.


Effects of intracoronary temperature variation on the coronary circulation.


Transmission electron microscopy of ion beam thinned dentine.


Stages and stage distribution in early oogenesis in the Annelid, Platynereis dumerilii.


The mediation of cellular events in delayed hypersensitivity reactions.


Panel discussion: Environmental sampling in an aseptic environment. II. Particulate contamination control.


Panel discussion: environmental sampling in an aseptic environment. I. Microbiological environmental monitoring.


The gene numbers game.

The gene numbers game.


The use of technetium-99m as a radioisotopic label to assess cell-mediated immunity in vitro.


Correlation of early changes in amino acid transport and DNA synthesis in stimulated lymphocytes.


Natural thymocytolytic autoantibodies in NZB and other strains of mice.


Dextran's regulatory effect on immunoglobulin synthesis is mediated through T cells.


Restoration of the in vitro antibody response of cortisone-treated spleen cells by T cells or soluble factors.


Allogeneic interactions in the immune response and tolerance. II. Mechanism of stimulation of TNP plaque forming cells in normal rats.


Functional and morphological heterogeneity among rabbit peritoneal macrophages.


Assessment of mitotic thymus-derived lymphocytes by their sensitivity to the cytotoxic effects of anti-theta serum.


Analysis of the temporal changes in the avidity of primary IgM and IgG antibody at the cellular level.


Unequal expression of allelic allotypic specificities in circulating immunoglobulins, experimentally-elicited antibodies, and receptor-carrying cells.


Acceleration of graft rejection by pretreatment of the graft donor with recipient cells (donor sensitization).


Antibody-induced lymphocyte-mediated cytotoxicity in sarcoidosis patients and healthy controls.


In vitro conversion of normal mouse lymphocytes by plasmacytoma RNA to express idiotypic specificities on their surface characteristic of the plasmacytoma immunoglobulin.


Analysis of the limiting-dilution assay used for estimating frequencies of immunocompetent units.


The management of the severely crushed chest by intermittent positive pressure ventilation.


Selective effects of mitogens on subpopulations of mouse lymphoid cells.


Comparison of three methods for the detection of Trichinella spiralis infections in pigs by five European laboratories. Three methods employed in the diagnosis of trichinosis (trichinoscopy, digestion method, and immunofluorescence technique) were compared by laboratories in 5 countries of the European economic community. For this purpose, material from 32 pigs infected with 50, 150, 500, and 1 500 T. spiralis larvae was examined. With none of the three methods was it possible to detect with sufficient reliability a T. spiralis infection in pigs infected with 50 larvae. The digestion method and the immunofluorescence technique yielded more reliable results when the infection dose was 150 larvae or more. With trichinoscopy, reliable results were obtained in pigs infected with 500 and 1 500 larvae. With the digestion method and trichinoscopy, the onset of infections was detectable from 3 weeks post infection, the digestion method being more reliable; the immunofluorescence technique yielded positive results from approximately 4-6 weeks post infection. The immunofluorescence technique is applicable for epidemiological surveys. As a routine diagnostic procedure in the slaughterhouse, trichinoscopy and the digestion method are possible alternatives, the latter being more sensitive.


Comparative studies on Salmonella isolations from feeds in ten laboratories. Pig feed samples, artificially and naturally contaminated with S. typhimurium, were distributed to ten laboratories. The isolation rates obtained by using a standardized pre-enrichment method and the laboratories' "own" methods were compared. In nearly all laboratories the standardized pre-enrichment method gave better results. Extending the duration of incubation of the enrichment medium to 48 h followed by a second subculture improved the isolation rates. In general, a pre-enrichment method should be used for the isolation of salmonellae, especially when they are injured or small numbers of them are expected in the material to be investigated.


Pathobiological relationships between Mycobacterium leprae and its primitive host. Newborn snakes were injected with 10(2)-10(4) live or heated Mycobacterium leprae. Death occurred in 5-6 weeks. On autopsy, the snakes injected with live microorganisms showed pathological changes and numerous acid-fast bacteria were found in some organs. Material was also transferred from an experimentally infected snake to a group of normal newborn snakes, causing their death in 3 weeks. Extracts in phosphate-buffered saline, prepared from the tissues of infected snakes, were found to react with anti-M. leprae and anti-M. lepraemurium rabbit antisera. No immunodiffusion reactions were elicited by extracts from the organs of control snakes.


Linkage studies on DDT and dieldrin resistance in species A and species B of the Anopheles gambiae complex. Linkage studies in A. gambiae species A showed that the two autosomal markers diamond (Di) and collarless (c) belonged to different linkage groups: Di on linkage group 2 and c on linkage group 3. With the aid of the two markers it was possible to establish the linkage relationship between the genes for DDT resistance and for the two types of dieldrin resistance. The DDT resistance gene in species A assorted independently of c and was found to be linked with Di at a map distance of 26.3 units. The genes for the two types of dieldrin resistance, dominant and incompletely dominant, were found to be allelic. The major gene for dieldrin resistance was shown to be linked with the gene for DDT resistance in linkage group 2 at a map distance of 35.3 units. This suggested that Di and the dieldrin resistance gene were some 61.6 units apart.


Innovation and creativeness in scientific research: my experiences in developing computerized axial tomography.


The ACTA-scanner: the whole body computerized transaxial tomograph.


An application of cluster analysis to the construction of a diagnostic classification.


Computer assisted instruction for teaching clinical decision-making.


Occurence of protein A among Staphylococcus aureus strains and its relation with some agglutinogens and enzymes.


The haemodynamic effects of quazodine, a cardiac stimulant, in experimental E. coli endotoxin shock in the cat.


The taxonomy of Staphylococcus aureus.

The taxonomy of Staphylococcus aureus.


Purification of staphylococcal beta-, gamma- and delta-hemolysins.


Physical properties of staphylococcal alpha-toxin and aspects of alpha-toxin membrane interactions.


Neurotoxicity of staphylococcal alpha- and beta-hemolysins.


Enterotoxigenic staphylococci and enterotoxin in human milk.


Current status of staphylococcal leukocidin research.


The application of crystal violet ring test to the detection of hospital Staphylococcus aureus strains.


Subdivision of Staphylococcus aureus for epidemiological purposes.


Staphylococcal interference.

Staphylococcal interference.


Occurence of protein A in strains of Staphylococcus aureus demonstrated by means of labelled globulins.


The immunofluorescence adsorption test (IFAT) in the estimation of staphylococcal protein A.


Effect of freezing and thawing on a moderately halophilic bacterium as a function of Na+, K+, and Mg2+ concentration.


Survival of electrical activity of deep frozen fetal mouse hearts after microwave thawing.


Elution of cell-bound anti-prostatic epithelial antibodies after multiple cryotherapy of carcinoma of the prostate.


Renal preservation by hypothermic perfusion. 2. The influence of oxygenator design and oxygen tension.


Self-concept and mother-concept in black and white preschool children.


Kinetic-family-drawings of children with diabetes.


Flexible fiberoptic bronchoscopy in critical care medicine. Diagnosis, therapy and complications.


New diuretics - bumetanide and metolazone.


Benapryzine for parkinsonism.

Benapryzine for parkinsonism.


Fenoprofen.

Fenoprofen.


Drugs for acute pancreatitis: aprotinin and glucagon.


Comparison of "Duphalac" and "irritant" laxatives during and after treatment of chronic constipation: a preliminary study. A multi-centre trial was carried out to compare the effectiveness in the treatment of chronic constipation of a lactulose preparation ("Duphalac") and "irritant" laxatives containing senna, anthraquinone derivatives or bisacodyl. The results, in 164 patients, indicate that the lactulose prepartion was more effective than the "irritant" laxatives. By Day 7, 58% of the lactulose-treated group were passing a normal stool whereas only 42% of the patients receiving an "irritant" laxative did so at the same stage in treatment. The lactulose preparation was shown to have a persistent CARRY-OVER" EFFECT, AND THIS EFFECT WAS SEEN IN SIGNIFICANTLY MORE PATIENTS RECEIVING "Duphalac" than in the "irritant" laxative group.


Oxolinic acid in urinary tract infection: a multi-centre trial. A single-blind trial of oxolinic acid (750 mg. twice-daily for 14 days) and ampicillin (500 mg. 3-times daily for 14 days) in 60 cases with urinary tract symptoms showed superior results with oxolinic acid: 94% of pathogens were sensitive to oxolinic acid and 69% were sensitive to ampicillin. Sympotomatic relief and bacterial eradication occurred earlier in the oxolinic acid group.


A double-blind comparison of chlormethiazole and dichloralphenazone: a sedative/hypnotic in geriatric medicine. In a double-blind crossover trial, chlormethiazole was compared with dichloralphenazone and placebo in 38 elderly patients requiring night-time sedation. Chlormethiazole was significantly superior to dichloralphenazone and placebo for the parameters 'heavy sleep' and'time of onset of sleep'. The incidence of side-effects did not significantly differ whilst on placebo. Because of the high incidence of "placebo reactors', a sub-group who slept badly whilst on placebo were further analysed. The data indicated a definitive trend suggesting that the difference found in the overall material was valid for the sub-group of 17 subjects. Problems of placebo reactors in clinical trials are discussed and the view supported that 'placebo reactors' should be excluded by a preliminary screening test before admission to double-blind controlled trials evalvuating hypnotic drugs.


Naproxen (Naprosyn) and ketoprofen (Orudis).


A comparison between chlormethiazole and nitrazepam as hypnotics in psycho-geriatric patients. A double-blind crossover study was carried out in 68 demented elderly patients (mean age 77 years) to compare the hypnotic effects of chlormethiazole and nitrazepam. Chlormethiazole was administered as a 5% mixture (500 mg. chlormethiazole edisylate) in a 10 ml. dose: the corresponding single dose of nitrazepam was 10 mg. Treatment was discontinued in 6 patients and interrupted for from 1 to 3 days in a further 18 due to side-effects and 'hang-over' problems or because of intercurrent infections. Of these 24 drop-outs, 3 occurred during chlormethiazole treatment (1 severe 'hang-over'; 2 refused to take medication) and 21 during nitrazepam (15 severe 'hang-over' effects, including sleepiness and muscular weakness; 2 nausea; 4 intercurrent infection).  Both preparations were equally effective as hypnotics, there being no noteworthy differences in time of onset or in duration of sleep. Of the 44 patients completing the trial without interuption, observations were carried out for  308 nights on each preparation. Chlormethiazole patients slept for more than 6 hours on 244 of the 308 nights without 'hang-over' effect the next day compared with 163 out of 308 nights of those on nitrazepam. The difference is statistically significant in favour of chlormethiazole. The high incidence of 'hang-over' effect during nitrazepam treatment indicates that a single 10 mg. dose is too large for use in the elderly. Overall assessment of treatment was made in 62 patients. Chlormethiazole was judged to be the most suitable drug in 37, nitrazepam in 11, and both preparations equally useful in the remaining 14 patients. This difference is statistically significant.


Controlled double-blind trail of flavoxate in painful conditions of the lower urinary tract. A double-blind, placebo-controlled trial of the antispasmodic flavoxate (600 mg. daily) was carried out in 60 patients suffering from painful conditions of the lower urinary tract arising from inflammation and/or infection or from spasm following diagnostic or therapeutic procedures. Statistically significant improvement was noted in frequency and suprapubic pain, and marked relief was also afforded in dysuria, hesitancy and burning. Apart from dryness of the mouth, the incidence and type of side-effects reported were similar in both groups of patients and were probably not drug related. Fewer patients interrupted treatment with flavoxate because of inefficacy than in the placebo group, the difference being statistically significant.


Current status of immunotherapy.

Current status of immunotherapy.


Current status of immunotherapy of human cancers: leukaemias, lymphomas, solid tumours.


Malignant lymphoreticular diseases at Kenyatta National Hospital in 1973.


Preliminary results of a trial in amoebic liver abscess: diagnosis by latex agglutination test and dry-blood sample serology. Treatment with tinidazole (Fasigyn).


The treatment of trichomonal vaginitis using a single dose of tinidazole by mouth.


Uptake, distribution, and fate of 203Hg-ethylmercuric chloride in the guppy and the coontail.


Determination of total suspended particulate matter and airborne fiber concentrations at three fibrous glass manufacturing facilities.


Analysis of the effect of fluorescence intensity on distributions obtained by flow cytofluorometry.


Changes in the G0 state of WI-38 fibroblasts at different times after confluence.


Separation of lymphoid-line cells according to volume and density.


Antibody-induced redistribution of surface antigen is independent of the amount of antigen and the cell cycle position.


Biochemical differentiation of a murine ganglioneuroblastoma in tissue culture.


Serum inhibitiors of in vitro colony formation: relation to haemopoietic tissue in vivo.


The relationship between granulocytic and erythroid repopulating ability.


The chemical and biological properties of busulphan ("Myleran").


Computer analysis of evoked vestibular nystagmus in healthy subjects. 2. Caloric stimulation--sequential analysis.


Treatment of blastic transformation of chronic granulocytic leukemia by high dose cyclophosphamide, total body irradiation and infusion of cryopreserved autologous marrow.


Stimulation by human urine or plasma of granulopoiesis by human marrow cells in agar.


Comparative assessment of two antidiarrhoeal preparations and placebo in the treatment of acute, non-specific enterocholitis.


A comparison of hematopoiesis after treatment with antithymocyte serum and erythropoietin.


Gonadal hormones, the nervous system and behavior.


Neuroendocrine and neuroanatomic correlates of atypical sexuality.


Gonadal hormones and the developing brain.


Embryogenesis in vitro: an experimental model for the understanding of reproductive physiology and development in mammals.


Condom--an old method meets a new social need.


Vasectomy--old and new techniques.

Vasectomy--old and new techniques.


Luteinizing hormone (LH) release after single injections of a synthetic LH-releasing hormone (LH-RH) in the ewe at three different reproductive stages and comparison with natural LH release at oestrus.


John Zephania Holwell. A biographical note.


Localization of calcium-binding protein in intestinal tissue by immunofluorescence in normal, vitamin-D-deficient and uraemic subjects. Antiserum directed against calcium-binding protein isolated from human kidneys was used for the immunofluorescent localization of calcium-binding protein in human intestine and kidney. Frozen sections of intestine obtained by biopsy from normal persons were tested by the indirect fluorescent antibody technique. Specific fluorescence indicating the presence of calciumbinding protein was observed at both the basal and apical poles of the intestinal absorptive cells while the goblet cells appeared to fluoresce non-specifically. Treatment of rachitic children with 25-hydroxycholecalciferol generally restored the pattern of fluorescence seen in intestinal tissue from normal persons. Examinations of intestinal biopsies from uraemic patients yielded variable results.


Ultraviolet mutagenesis and its repair in an Escherichia coli strain containing a nonsense codon. Ultraviolet mutagenesis and its repair were studied mainly in WU36-10-89, a uvr(-) strain of Escherichia coli containing a UAG mutation in a gene for leucine biosynthesis. Following ultraviolet (UV) irradiation revertants appearing with or without direct photoreactivation (PR) were classified according to the presence and type of suppressor they contained. We find UV mutation production to be quite specific. An analysis of revertants produced by UV indicates they are formed mainly from GC --> AT and that the miscoding is due to a cytosine residue at the site of mutation in a cytosine-thymine (CT) dimer. We propose that the dimer serves as template during some aspects of repair replication and at the time of replication the C in the dimer directs the insertion of A in the complementary strand. We also note that C --> A and T -->G changes caused by a CT dimer occur much less frequently.


The memoirs of Dr Henry Shinglewood Taylor.


The effect of benzoctamine on the ventilatory response to carbon dioxide in normal subjects and in patients with chronic airways obstruction.


Endometrial morphologic response to hormonal environment.


Cytologic methods for the detection of endometrial cancer.


Histologic screening for endometrial cancer.


Adjuvant progestogen therapy in the primary definitive treatment of endometrial cancer.


Professor Kazimierz Mietkiewski 1906-1973.


The treatment of advanced endometrial cancer with hydroxyprogesterone caproate.


Ovulation and implantation in montane voles (Microtus montanus) as a function of varying amounts of copulatory stimulation.


Effect of various organic compounds on the synthesis of pectinases by Aspergillus niger JU.


Effects of variations in the male copulatory behavior on ovulation and implantation in prairie voles, Microtus ochrogaster. The effects of copulation on ovulation and implantation were studied in the prairie vole (Microtus ochrogaster).  Both 1 and 2 ejaculatory series induced ovulation in 9 of 10 females.  2 ejaculatory series resulted in slightly fewer corpora lutea and implanted embryos, and in a slightly greater incidence in intrauterine mortality.  In the first ejaculatory series, the likelihood of ovulation increases with increased numbers of intromissions and ejaculatory thrusts.  Vaginal penetration is required for the induction of ovulation.  The congruence of male copulatory behavior and female reproductive physiology would seem to indicate a coadapatation among this species.


Haemolysin and necrotoxin production by Escherichia coli.


Immunological studies in protein malnutrition.


A simple morphometric method for the study of placentae.


Immune and non-immune defence mechanisms in protein deficiency.


A morphometric study of placenta from normal and toxaemic cases.


Ernesto Scoffone. In memoriam.

Ernesto Scoffone. In memoriam.


Radiosensitization of biologically active DNA in cellular extracts by oxygen. Evidence that the presence of SH-compounds is not required.


Metronidazole ('Flagyl'): mechanisms of radiosensitization.


The effect of pre-irradiation growth conditions on the relative radiosensitivities of mammalian cells at low oxygen concentrations.


Chromosomal proteins.

Chromosomal proteins.


The effect of methylphenidate on sensory perception and integration in hyperactive children.


Comparative aspects of the ultrastructure of the female gamete.


Transfer of genetic information in polytene cells.


Eukaryotic DNA polymerases: their association with the nucleus and relationship to DNA replication.


Structure, biochemistry, and functions of the nuclear envelope.


Malaria antibody titres and parenchymal neurosyphilis.


Historical perspectives on the development of psychoanalytic psychotherapy.


Obituaries.

Obituaries.


Psychological insight between enlightenment and early romanticism.


New directions in genetics utilizing tissue culture techniques.


The fluorescent pattern of normal chromosomes in biopsies of malignant lymphomas, and its computer display.


The temperature influence on the spontaneous mutation rate. I. Literature review.


The chromosomes of an in vitro derivative of an Ehrlich ascites tumor of the mouse during its adaptation from monolayer to suspension culture.


Picric acid-formaldehyde fixation for immunoferritin studies.


Jolande Jacobi.

Jolande Jacobi.


Bruno Klopfer.

Bruno Klopfer.


Metabolic products products from Spicaria divaricata NRRL 5771.


Studies on bredinin. I. Isolation, characterization and biological properties.


Metabolic products of microorganisms 133. Inhibition of ribosomal peptidyl transferase by hikizimycin, a nucleoside antibiotic.


Botric acid, a new antibiotic.

Botric acid, a new antibiotic.


Aspiculamycin, a new cytosine nucleoside antibiotic. IV. Antimycoplasma activity of aspiculamycin in vitro and in vivo.


Letter: Inactivation of beta-lactamase by some site-specific reagents.


Synthesis of branced-chain aminoacyl-transfer ribonucleid acid synthetases in a Salmonella typhimurium mutant with an altered biosynthetic L-threonine deaminase. The differential rates of synthesis of the three branched-chain aminoacyl-transfer ribonucleic acid synthetases were measured in Salmonella typhimurium LT-2 and a mutant, ilvA504. The mutant produced an l-threonine deaminase with a decreased affinity for its cofactor, pyridoxal-5'-monophosphate. The addition of pyridoxal-5'-monophosphate to cultures of strain ilvA504 growing in excess isoleucine, valine, and leucine resulted in an increased rate of growth and repression of the synthesis of the isoleucine and valine biosynthetic enzymes. No differences in the rate of synthesis of the branched-chain aminoacyl-transfer ribonucleic acid synthetases were observed in cultures of ilvA504 growing with or without added pyridoxal-5'-monophosphate. The differential rates of synthesis of all three enzymes were similar to the rates measured in strain LT-2. These experiments suggest that different forms of the ilvA gene product are involved in the regulation of the branched-chain amino acid biosynthetic enzymes and the branched-chain aminoacyl-transfer ribonucleic acid synthetases.


Isolation and characterization of a copy mutant of the bacteriocinogenic plasmid Clo DF13. After nitrosoguanidine mutagenesis, strain Escherichia coli P678-54, bacteriocinogenic for Clo DF13, yielded a mutant strain that showed an enhanced bacteriocin production. The results from conjugation experiments indicated that the mutation, responsible for the enhanced bacteriocin production, is located on the Clo DF13 plasmid. The following properties of strains harboring the mutant Clo DF13 plasmid could be observed. (i) The bacteriocin production in these strains can be further enhanced at least fourfold by mitomycin C. (ii) The fraction of spontaneously induced cells, as revealed by lacunae experiments, in cultures of these strains is about nine times higher than in cultures of wild-type Clo DF13-harboring strains. (iii) Chromosomeless minicells from strain P678-54 harboring the mutant Clo DF13 plasmid synthesize about six times more deoxyribonucleic acid, ribonucleic acid, and protein as compared to wild-type Clo DF13-harboring minicells. (iv) Analysis of this mutant Clo DF13-specific ribonucleic acid and protein on polyacrylamide gels revealed mainly the same ribonucleic acid and polypeptide species as synthesized by the wild-type Clo DF13 minicells, but in larger amounts (Kool et al., 1974). (v) Segregation experiments, using a strain with temperature-sensitive polymerase I, show that mutant Clo DF13-harboring cells contain an average of 70 Clo DF13 copies per cell, whereas wild-type Clo DF13-harboring cells contain only about 10 Clo DF13 copies per cell. The data presented in this paper indicate that the mutation on the Clo DF13 plasmid leads to an altered control of Clo DF13 replication and results in an enhanced number of Clo DF13 copies per cell. As a secondary effect, this enhanced number of Clo DF13 copies enhances the probability of "spontaneous" induction per cell. Since the mutation is plasmid specific and affects the number of plasmid copies produced, one can conclude that the Clo DF13 plasmid is not dependent solely on chromosomal information, but that at least plasmid base sequences are involved in Clo DF13 plasmid replication.


Characterization of a dominant, constitutive mutation, PHOO, for the repressible acid phosphatase synthesis in Saccharomyces cerevisiae. An apparent operator-constitutive mutation was discovered in the repressible acid phosphatase system in Saccharomyces cerevisiae. The site of mutation, designated PHOO, was found to be closely linked to the phoD locus. The mutant allele, PHOO, was semidominant over the wild-type allele and effective for the expression of the phoD gene in cis position. The phoD mutation gave rise to a defective phenotype for the formation of the repressible acid phosphatase. On the other hand, neither the repressible acid phosphatase activity in the cell-free extracts prepared from cells of the temperature-sensitive phoD mutant grown at 25 C, nor that of the revertants from the phoD mutants, could be distinguished from that of the wild-type strain with respect to thermolability and K(m) value for p-nitrophenylphosphate. These results strongly suggest that the phoD gene is not a structural gene, but a regulatory gene exerting positive control for the formation of repressible acid phosphatase. Close similarity between the apparent role of the phoO-PHOD gene cluster and that of the c-GAL4 gene cluster in the galactose system of S. cerevisiae could be inferred.


Comparison of the deoxyribonucleic acid molecular weights and homologies of plasmids conferring linked resistance to streptomycin and sulfonamides. Bacterial strains showing linked resistance to streptomycin (Sm) and sulfonamides (Su) were chosen representing a wide taxonomic and geographical range. Their SmSu resistances were transferred to Escherichia coli K-12 and then plasmid deoxyribonucleic acid (DNA) was isolated by ethidium bromide CsCl centrifugation. The plasmid DNA was examined by electron microscopy and analyzed by sedimentation through 5 to 20% neutral sucrose gradients. Plasmid DNA from strains having transmissible SmSu resistance consisted of two or three molecular species, one of which had a molecular mass of about 5.7 Mdal (10(6) daltons), the others varying between 20 to 60 Mdal. By using transformation or F' mobilization, we isolated the SmSu-resistance determinant from any fellow resident plasmids in each strain and again isolated the plasmid DNA. Cosedimentation of each of these with a differently labeled reference plasmid DNA (R300B) showed 9 out of 12 of the plasmids to have a molecular mass not significantly different from the reference (5.7 Mdal); two others were 6.3 and 9.2 Mdal, but PB165 consisted of three plasmids of 7.4, 14.7, and 21.4 Mdal. Three separate isolations of the SmSu determinant from PB165 gave the same three plasmids, which we conclude may be monomer, dimer, and trimer, respectively. DNA-DNA hybridizations at 75 C demonstrated 80 to 93% homology between reference R300B DNA and each isolated SmSu plasmid DNA, except for the 9.2-Mdal plasmid which had 45% homology and PB165 which had 35%. All the SmSu plasmids were present as multiple copies (about 10) per chromosome. The conjugative plasmid of R300 (present as 1.3 copies per chromosome) has been shown to have negligible effect on the number of copies of its accompanying SmSu plasmid R300B. We conclude that the SmSu plasmids are closely related and probably have a common evolutionary origin.


Subcellular localization of isoleucine-valine biosynthetic enzymes in yeast. By using the method of stepwise homogenization of yeast spheroplast lysates employed previously with the leucine biosynthetic enzymes, it is shown that threonine deaminase, acetohydroxy acid synthase, Mg(2+)-dependent isomero-reductase, and dihydroxy acid dehydratase are particulate. Density gradient centrifugation and the behavior of marker enzymes suggest that all of the above enzymes of the isoleucine-valine biosynthetic pathway are associated with the mitochondria.


Regulation of the beta-glucoside system in Escherchia coli K-12. In Escherichia coli wild-type cells, a mutation at the beta-glucoside regulatory gene (bglR(+) to bglR(-)) leads to simultaneous expression of inducible phospho-beta-glucosidase B (bglB(+)) and a beta-glucoside-specific species of enzyme II (beta-glucoside transport I [bglC(+)]); an additional mutation (bglS(+) to bglS4) allows these enzymes to be formed constitutively. The bgl alleles have been mapped in the following order: pyrE, bglA, bglB, bglS, bglR, bglC, ilvD. The back mutation in the regulatory allele (bglR(-) to bglR(+)) caused the cessation of the expression of the bglB(+), bglS(+) or bglS4, bglC(+) alleles. However, a mutation in a strain with bglB(+), bglS4, bglR8, bglC(+) alleles, at the ini site that lies between the bglS4 and the bglR8 allele, allowed the expression of the bglS4 and bglB(+) alleles, but showed no affect on the expression of the bglC(+) allele. It is suggested that the ini mutation possesses a promotor-type function that in the absence of regulatory allele function (bglR8) renews the functioning of only the bglS4 and bglB(+) alleles. The complementation studies have shown that the bglB(+), bglS(+) or bglS4, bglC(+) alleles are expressed only in cis to the bglR(-) allele. In the constitutive strain (bglB(+), bglS4, bglR(-), bglC(+)), the expressed bglS4 allele formed a soluble product that acts in trans over the bglB(+) and bglC(+) alleles and that appears effective only when the bglB(+) and the bglC(+) alleles are expressed in cis to the bglR(-) allele. It thus showed that the constitutive biosynthesis of phospho-beta-glucosidase B and beta-glucoside transport I is under positive control. Since the regulatory allele bglR(-) lies between the bglS4 and the blgC(+) alleles, and acts in cis, it appears that the mutation (bglR(+) to bglR(-)) allows the initiation of transcription in one direction to express the bglS4, bglB(+) alleles and in the other to express the bglC(+) allele. The structural genes bglB and bglC lie adjacent to the regulatory genes bglR and bglS, and the structural genes are coordinately controlled by the regulatory genes. It is, therefore, proposed that the bglB, bglS, bglR, bglC genes form a bgl operon.


Methylation of ribosomal proteins in Escherichia coli. Escherichia coli was grown in a medium containing [1-(14)C]methionine and [methyl-(3)H]methionine, and the (3)H/(14)C ratio was determined for each of the ribosomal proteins derived from the 70S ribosome. Evidence indicates that six proteins from the 50S subunit were methylated: L7, L9, L11, L12, L18, and L33. Methylation of several other 50S proteins (such as L1, L3, L5, etc.) may also occur. The methylated amino acids in protein L11 have been characterized further and found to be predominately epsilon-trimethyllysine. A small amount of a compound tentatively identified as N(G), N'(G)-dimethylarginine was also detected.


Regulation of the synthesis of the lactose repressor. Measurements of the lactose repressor over a tenfold range of cell growth rates were made on protein extracts from Escherichia coli cultures grown in media with various carbon energy sources. The concentration of lactose repressor varied with the number of genome equivalents per cell over this range in growth rates, suggesting that the number of lactose molecules within the cell is determined by the number of I gene copies present. The timing of repressor synthesis during the cell division cycle and its correlation with deoxyribonucleic acid synthesis was examined by synchronizing the cell division cycle of E. coli ED1039, in which the Lac region has been transposed from 10 to 36 min on the genetic map. Measurements of lactose repressor in the synchronized culture revealed a burst of repressor synthesis at the time of I gene duplication. The concentration of lactose repressor was found to decrease as a function of total cell protein during the division cycle until an increase in synthesis occurred, suggesting that repressor synthesis probably does not occur throughout the division cycle. A model for I gene regulation is proposed.


Isoleucine and valine metabolism in Escherichia coli K-12: detection and measurement of ilv-specific messenger ribonucleic acid. Ribonucleic acid-deoxyribonucleic acid (RNA-DNA) hybridization was employed for the determination of messenger RNA transcribed from the ilv gene cluster of Escherichia coli K-12. Strains with derepressed levels of the isoleucine and valine biosynthetic enzymes owing to linked or unlinked genetic lesions were found to exhibit ilv messenger RNA levels from 1.5- to 4-fold higher than did their isogenic parents. When grown under conditions that specifically repressed the synthesis of isoleucine- and valine-forming enzymes, most strains exhibited drastically reduced ilv messenger RNA levels. Hybridization performed with the separated strands of ilv DNA showed that all the ilv genes are transcribed from the same strand, the "l strand" of lambdaphi80CI857St68dilv DNA. Sucrose gradient analyses of RNA extracted from cells starved for isoleucine, valine, or leucine resulted in the detection of at least two distinct types of ilv messenger RNA.


Regulation of transaminase C synthesis in Escherichia coli: conditional leucine auxotrophy. The regulation of synthesis of the valine-alanine-alpha-aminobutyrate transaminase (transaminase C) was studied in Escherichia coli mutants lacking the branched-chain amino acid transaminase (transaminase B). An investigation was made of two strains, CU2 and CU2002, each carrying the same transaminase B lesion but exhibiting different growth responses on a medium supplemented with branched-chain amino acids. Both had the absolute isoleucine requirement characteristic of ilvE auxotrophs, but growth of strain CU2 was stimulated by valine, whereas that of strain CU2002 was markedly inhibited by valine. Strain CU2002 behaved like a conditional leucine auxotroph in that the inhibition by valine was reversed by leucine. Results of enzymatic studies showed that synthesis of transaminase C was repressed by valine in strain CU2002 but not in strain CU2. Inhibition by valine in strain CU2002 appears to be the combined effect of repression on transaminase C synthesis and valine-dependent feedback inhibition of alpha-acetohydroxy acid synthase activity, causing alpha-ketoisovalerate (and hence leucine) limitation. The ilvE markers of strains CU2 and CU2002 were each transferred by transduction to a wild-type genetical background. All ilvE recombinants from both crosses resembled strain CU2002 and were inhibited by valine in the presence of isoleucine. Thus, strain CU2 carries an additional lesion that allows it to grow on a medium containing isoleucine plus valine. It is concluded that conditional leucine auxotrophy is characteristic of mutants carrying an ilvE lesion alone.


Effect of altered sterol composition on growth characteristics of Saccharomyces cerevisiae. The function of sterols in mitochondrial structures of yeast was examined. Sterol mutant strains were employed to examine the effects of altered sterolic content on optimal and permissive growth temperatures in respiring and fermenting cultures. Although fermentative growth was unaffected by sterol composition, a definite decrease in both the optimal and the permissive growth temperatures of respiring cultures was observed when ergosterol was replaced by Delta(8(9), 22)-ergostadiene-3beta-ol. In vitro studies showed a similar decrease in membrane phase transition temperatures of the mitochondrial enzyme S-adenosylmethionine: Delta(24)-sterol methyltransferase in the mutant strains. Increased sterol and methyltransferase levels were detected in strains incapable of synthesizing ergosterol. A possible control function governing sterol synthesis is proposed for ergosterol.


Active transport of biotin in Escherichia coli K-12. The transport of [(14)C]biotin into cells of a biotin prototroph, Escherichia coli K-12 strain Y10-1, was investigated. The vitamin taken up by the cells in this strain existed primarily in the free form. Addition of glucose enhanced the rate of uptake six- to eightfold and the steady level was reached in 2 to 3 min resulting in accumulation of biotin against a concentration gradient. The uptake showed marked dependence on temperature (Q(10), 2.3; optimum, 37 C) and pH (optimum 6.6) and was inhibited by iodoacetate. Energy of activation for glucose-dependent uptake was calculated to be 16,200 cal per mol. The rate of biotin uptake with increasing biotin concentrations showed saturation kinetics with an apparent K(m) and V(max) values of 1.4 x 10(-7) M and 6.6 pmol per mg of dry cells per min respectively. The cells also accumulated biotin against a concentration gradient in the absence of added glucose, although at a much lower rate. This accumulation was much more susceptible to inhibition by azide and uncouplers of oxidative phosphorylation suggesting that the energy source was supplied through the electron-transport chain. Inhibition studies with a number of biotin analogues indicated the requirement for an intact ureido ring. The biotin uptake was inhibited in cells grown in biotin-containing medium and was shown to be the result of repression of the transport system, suggesting the control of the biotin transport.


Nature of ribonucleic acid synthesis during early sporulation in Saccharomyces cerevisiae. Phosphate uptake in sporulating cultures of Saccharomyces cerevisiae has been found to occur approximately 2 h after the transfer to sporulation medium. Early ribonucleic acid synthesis begins at approximately 4 h and continues to 8 h. Incorporation of phosphate into acid-extractable precursor pools parallels phosphate uptake. In triple-labeling experiments it was observed that the breakdown of vegetatively synthesized ribonucleic acid is not a significant source of precursors for ribonucleic acid synthesis during sporulation. The majority of the ribonucleic acid made in a 10-min period during sporulation does not migrate on gels with precursor or mature ribosomal ribonucleic acid.


The rep mutation. III. Altered structure of the replicating Escherichia coli chromosome. The rep gene function of Escherichia coli is essential for the replication of P2 and phiX174 double-stranded deoxyribonucleic acid (DNA). Compared with isogenic rep(+) strains, rep mutants show the following characteristics: larger cell size, more DNA per cell, and a slightly lower DNA/mass ratio. The replicating rep chromosomes show a steeper gradient of marker frequencies and contain more replicating forks per chromosome. The nucleoid body of rep mutants sediments faster and contains more DNA. We deduce that the rep function is required for the "normal" replication of the E. coli chromosome and that in its absence the E. coli chromosome replicates in an altered manner, perhaps involving slower-moving replicating forks.


Unimportance of counterflux in the energetics of "downhill" transport. Adam Kepes suggested that the cellular transport and hydrolysis of orthonitrophenyl-beta-d-galactopyranoside is powered by the counterflux of the d-galactose resulting from beta-galactosidase action within the cell. His explanation would rationalize the unique insensitivity of this galactoside transport to energy poisons such as azide. But contrary to the predictions of this hypothesis, (i) there is no initial large inhibition that progressively lessens as galactose is produced. This was shown with a double wavelength stopped-flow spectrophotometer developed to eliminate interference from turbidity transients. (ii) The azide sensitivity does not increase with an external concentration of galactose sufficient to reverse the thermodynamic gradient. (iii) Mutation in galactose utilization or growth on highly catabolite-repressing regimens did not increase the azide sensitivity, and induction of galactose transport and metabolism did not decrease azide sensitivity. It was found that Kepes measurements must have contained two artifacts. One is that the control rate of hydrolysis decreases with time as the dense cell suspension becomes anaerobic. The other is that azide causes turbidity changes for some time after its introduction. If the former is avoided by magnetic stirring and the latter by double wavelength spectrophotometry or controls without substrate, the inhibition is constant from the earliest time that can be measured. It is therefore concluded that energy-unstarved cells, exposed to azide, still have adequate energy reserves to couple to the downhill transport, although their potential is not adequate to drive accumulation against a concentration gradient.


His-linked hydrogen sulfide locus in Salmonella typhimurium. Investigation of strains of Salmonella typhimurium having extended his deletion mutations indicates that a genetic site (or sites) affecting H(2)S production is located in the region of the chromosome adjacent to the operator end of the his operon. This site is co-transducible with the his genes. Experimental data indicate that the site is also present on an F'his factor derived from S. typhimurium, FS401. Evidence is presented for the existence of another site affecting H(2)S production which is not linked to his.


Location of the genes controlling alkaline phosphatase on the F13 episome of Escherichia coli. Interrupted mating experiments between F'13 and F(-) cells showed that the location on the F'13 episome of the genes controlling alkaline phosphatase is on the end proximal to the point of entry, in the order phoA proC phoB phoR tsx.


Internal promoter P2 of the histidine operon of Salmonella typhimurium. The histidine operon internal promoter P2 allows initiation of transcription at a rate up to one-third that of the wild-type his operon.


Isolation and composition of oligosaccharide cores from endotoxins of two Serratia marcescens strains. The oligosaccharide cores isolated from the acetic acid hydrolysates of endotoxins from Serratia marcescens 08 and Serratia marcescens Bizio were analyzed for their sugar composition. The intact oligosaccharide core from S. marcescens 08 consisted of 2-keto-3-deoxyoctonate, d-glycero-d-mannoheptose, l-glycero-d-mannoheptose, d-glucose, d-galactose, and d-glucosamine in a molar ratio of 2:1:5:3:1:3 and that from S. marcescens Bizio consisted of the same sugar components in a molar ratio of 2:1:5:5:1:2. This result indicates that endotoxins from S. marcescens genus may contain more than one structural type of oligosaccharide core. Both oligosaccharide cores also differ in their chemical compositions from cores of other Enterobacteriaceae.


Transport of sugars and amino acids in bacteria. XII. Substrate specificities of the branched chain amino acid-binding proteins of Escherichia coli.


Kinetic properties of dissociation of the H-meromyosin-ADP complex.


Degradation of guinea pig IgG1 and IgG2 by splenic acid protease.


Studies on the regulatory functions of malic enzymes. III. Regulatory effects of L-aspartate, coenzyme A, and divalent metal ions on NAD-linked malic enzyme from Escherichia coli.


Amino acid sequence of L-asparaginase from Escherichia coli.


A combined metallurgical and histological study of tissue-prosthesis interactions in orthopedic patients.


A comparison of soft tissue reaction to silastic and absorbable suture material.


Histochemical responses at a biomaterial's interface.


The foreign body reaction--an experimental protocol.


Transcutaneous implants: reactions of the skin-implant interface.


Acquiring skill in reading: a review of experimental research.


Serum hepatitis as a persistent infection.


Subacute sclerosing panencephalitis.

Subacute sclerosing panencephalitis.


Creutzfeldt-Jakob disease.

Creutzfeldt-Jakob disease.


A rennin-sensitive bond in alpha-s1 B-casein.


Persistent infection of the mouse with the virus of lymphocytic choriomeningitis.


The effect of reduction and alkylation on the primary phase of rennin action on unheated and heated milk.


Influence of dietary proteins on rennin and pepsin content of preruminant calf vell.


The role of beta-lactoglobulin in the primary phase of rennin action on heated casein micelles and heated milk.


Measurements in vivo of the rate of production of protozoa in the rumen.


Letter: Two improvements in two-part denture design.


A method for the quantitative analysis of the enzyme complement of commercial rennets.


On the etiology of neurosis.

On the etiology of neurosis.


Problems in Freud's psychology of women.


The adolescent orphan in literature a bibliography for the study of the adolescent.


One proposal on staging of reticulum cell sarcoma from a view point of radiotherapy.


Rational use of psychotropic drugs. IV. Antidepressants.


Maxillary osteotomy in early childhood. A preliminary report.


The development of the cleft upper jaw following primary osteoplasty and orthodontic treatment. (A report on 87 cases).


In memoriam Prof. Charles Freidel, M.D., D.M.D. Vice-president of the E.A.M.F.S.


A theoretical mechanism of spirochete locomotion.


Lumbosacral agenesis: management and embryological implications. Rectal biopsy in a case of lumbosacral agenesis showed the presence of autonomic nerve elements normally supplied by spinal levels distal to the agenesis. Certain implications are suggested regarding the embryogenesis of this condition from data about the development of neural, bony, and muscular elements. Recommendations regarding management are given.


Role of intestinal microbes on body nitrogen accumulation in germfree, gnotobiotic and conventional mice.


Proceedings: Identification of periventicular cells projecting to the median eminence.


Proceedings: Synaptic and micro-iontophoretic inhibition of units in the rostral hypothalamus.


Transport of rubidium and sodium in pancreatic islets. 1. Fluxes of (86)Rb(+) and (22)Na(+) were measured in pancreatic islets of ob/ob-mice. The islets, which contain more than 90% beta-cells, were incubated at 37 degrees C in Krebs-Ringer bicarbonate buffer with modifications known to influence insulin release.2. In the presence of Na(+), the islets vigorously accumulated Rb(+). The Rb(+) uptake was inhibited by depletion of islet Na(+) or by 1 mm ouabain or 0.1 mm chloromercuribenzene-p-sulphonic acid. Rb(+) uptake was stimulated by 1 mm-5,5'-dithiobis (2-nitrobenzoic acid) or by depletion of islet Ca(2+), while 20 mm glucose, 5 mm theophylline, 0.1 mm iodoacetamide, or 1 mm-6,6'-dithionicotinic acid had no significant effects.3. The efflux of Rb(+) from preloaded islets followed exponential kinetics with a half-life of about 16 min. The rate of efflux was enhanced by 0.1 mm chloromercuribenzene-p-sulphonic acid and inhibited by 20 mm glucose. Omission of Na(+), K(+) or Ca(2+) from the incubation medium had no significant effects.4. The efflux of (22)Na(+) from islets preloaded with this isotope was enhanced by 0.1 mm chloromercuribenzene-p-sulphonic acid or by Ca(2+) deficiency. It was inhibited by 1 mm ouabain, 0.1 mm-2,4-dinitrophenol, or by omission of Na(+) from the incubation medium. Omission of K(+) or the addition of 20 mm glucose had no significant effects.5. It is concluded that the beta-cells are permeable to Na(+) and Rb(+) and expel Na(+) by an active mechanism similar to, or identical with, the Na(+)/K(+)-pump in other cells. The mechanisms of active and passive cation movements are discussed in relation to current hypotheses of stimulus-secretion coupling in the beta-cells depending on interactions between Na(+) and Ca(2+). In particular, the results support the hypotheses of insulin release being stimulated by ouabain through inhibition of the Na(+)/K(+)-pump and by organic mercurials through enhancement of membrane permeability to cations.


Proceedings: Iontophoretic and intravenous effects of the neuroleptic, alpha-flupenthixol, on dopamine evoked inhibition.


Electrophysiological evidence for the activation of supraoptic neurones during the release of oxytocin. 1. Antidromically identified supraoptic (SO) units were recorded in lactating rats anaesthetized with urethane (1.1 g/kg), and their activity was studied during milk ejection evoked by the suckling of the young. Fifty-eight SO units were recorded through 174 milk ejections. Each milk ejection was the result of a neurohypophysial release of an oxytocin pulse of 0.5-1.5 m-u.2. Fifty-five of the SO units displayed background activity and three were silent. The firing rates ranged from 0 to 15.4 spikes/sec, the distribution was exponential with 26% of the units firing at < 1 spike/sec. Sixteen (28%) of the SO units displayed a phasic pattern of activity characterized by periods of activity (6-132 sec) and silence (4-71 sec).3. Twenty-five of the non-phasic units displayed a large and stereotyped acceleration in spike activity some 10.5-17.4 sec before the rise in intramammary pressure at milk ejection. Units accelerated to rates between 9-66 spikes/sec, an increase of about thirtyfold (median) on background activity. The response was brief (0.9-4.7 sec) and was followed by a period of after-inhibition.4. It was concluded from studies of double recordings and observations of multi-unit activity that all the responsive units were synchronously activated. The mean latency of 13.3 sec between the onset of the neurosecretory response and milk ejection was similar to that observed following the experimental release of endogenous oxytocin by electrical stimulation of the neurohypophysis (50 pulses/sec for 2-4 sec).5. Four of the phasically active units were correlated with the oxytocin release for milk ejection. Three of these units displayed a burst of activity superimposed on the terminal portion of an active phase, some 10.2-14.7 sec before milk ejection. The fourth unit, recorded in conjunction with a responsive non-phasic unit, consistently switched from silence to activity coincident with the onset of the SO activation.6. The remaining SO units and a further ten units that were not antidromically activated by neurohypophysial stimulation displayed no change in activity during either the period of neurosecretory activation or the period of after-inhibition.7. This activation of the SO neurones, in the formulation of oxytocin release and milk ejection, is the same as that we have previously observed in recordings from the paraventricular (PV) region, and the proportion of neurones displaying the response is similar: 48% in the SO nuclei, 58% in the PV nuclei. We conclude, since the SO nuclei contain 80% of the neurosecretory cells that project to the neurohypophysis, that the SO nuclei are as important, if not more so, than the PV nuclei in the control of oxytocin release.


In vivo detoxification of endotoxin by mouse liver.


Late-stage lupus nephropathy.

Late-stage lupus nephropathy.


Bibliography on antibiotics. CV. (overseas publication).


Radioisotope dilution method for determination of prostaglandin F2alpha in rat tissue.


Antiulcerogenic property of sodium polyacrylate on experimental ulcerations in rats.


Stochastic processes in messenger RNA turnover.


Dynamics of chemostat culture:the effect of a delay in cell response.


Psychosocial aspects of vasectomy in Latin America. Social constraints, the structure of the Latin American family, and the "machismo" cult are some of the reasons for the scarcity of vasectomy programs in Latin America.  If processes of screening and counseling, and selfselection are employed, vasectomy programs can be just as successful in Latin America as in other countries.  Some psychological factors will always have to be considered.  One theory by Desmond Morris holds that the primitive behavior patterns of man, which evolved to protect the young, still persists today although they are no longer necessary.  One study showed that men who had received vasectomies were socially more aggressive and assertive as compensatory behavior.  The Spanish tradition of patriachy combined with the Roman Catholic tradition of male dominance determines the culture in which machismo is a natural outgrowth.  The woman is regarded as property.  For the woman, bearing children is the reason for existence.  It is possible that all men view vasectomy as castration on the subconscious level.  In most Latin American countries the laws are against voluntary contraceptive sterilization, but the laws are not enforced.


In memoriam: George Harold Kern (1903-1974).


In memoriam: Mario Galeazzi (1910-1974).


Computer-assisted clinical decision-making.


A bayesian method for computer-aided medical diagnosis.


A discriminatory analysis system in a health screening project.


Some observations of the procedures used in medical diagnosis in real-life situations and under artificial simulated conditions.


Medical computer records in relation to the definition of disease.


Douglas Gordon Carruthers.

Douglas Gordon Carruthers.


Utilization of histidine in vivo by Escherichia coli B.


Studies on the accumulation of ergosterol and 24(28)-dehydroergosterol in 3 strains of Saccharomyces cerevisiae.


A minute colony mutant of yeast.

A minute colony mutant of yeast.


Tetracyclines and host defence mechanisms. Doxycycline interference with phagocytosis of Escherichia coli.


Control of gene expression in bacteriophage T7. Isolation of a new control protein and mechanism of action.


Synthesis and stability of individual ribosomal proteins in the presence of rifampicin.


Recombination and segreation of mitochondrial genes in Saccharomyces cervisiae.


Segregation of mitochondially inherited antibiotic resistance genes in zygote cell lineges of Saccharomyces cerevisiae.


Stabilization of mRNA with polar effects in an Escherichia coli mutant.


A simple procedure for localized mutagenesis using nitrosoguanidine.


Cold-sensitive mutants in Aspergillus nidulans. II. Mutations affecting ribosome production.


A method for selective cloning of eukaryotic DNA fragments in Escherichia coli by repeated transformation.


Conjugational synthesis of F lac+ and Col I DNA in the presence of rifampicin and in Escherichia coli K12 mutants defective in DNA synthesis.


The genetic location of the sbcA gene of Escherichia coli.


Some functions involved in bacteriophage T7 genetic recombination.


Estimation of the D period from residual division after exposure of exponential phase bacteria to chloramphenicol. Values of the D period, between termination of chromosome replication and cell division, were determined from measurements of residual cell division after exposure of exponential phase cultures of Escherichia coli B/r and K12 and of Salmonella typhimurium to chloramphenicol. The results obtained by this method were compared with earlier results for E. coli B/r obtained from measurements of DNA content per cell and were found to be almost identical. For each, values of the D period were independent of growth rate, and the average value of D-26.1 plus or minus 1.2 min obtained by residual division is in good agreement with the value of 25 min obtained earlier. These results indicate that the method of residual division provides a good measure of the duration of the D period. Values of D were also independent of growth rate for each of the other strains.


Different effects of rifampicin and streptolydigin on the control of RNA polymerase subunit synthesis in Escherichia coli.


Competition for DNA polymerase III between the chromosome and the R-factor R1.


Cold-sensitive mutants in Aspergillus nidulans. I. Isolation and general characterisation.


Pleiotropic effects of ribosomal protein s4 studied in Escherichia coli mutants.


Genetic studies of erythromycin resistant mutants of Escherichia coli.


The size of mitochondrial DNA from a cytoplasmic petite mutant of Saccharomyces cerevisiae.


Efficiency of paraffin bait technique in the isolation of Nocardia from bronchopulmonary disorders.


Effect of goldthioglucose on long-term cultures of mouse hypothalamus.


Influence of striatal dopamine receptor blockade on a bulbar motor reaction.


Blockade of pyrogen induced fever by intrahypothalamic injections of salicylate in the rat.


Chemical stimulation of the amygdala with special regard to influence on the hypothalamus.


The use of medium-chain triglyceride in children with intestinal malabsorption.


The preputial gland of the male mouse.

The preputial gland of the male mouse.


Corticosteroid and immunosuppressive treatment in glomerulonephritis. (Literature review).


Immunopathology and microvascular lesions in human sympathetic ganglia. Lumbar sympathetic ganglia surgically removed from adult patients affected by obstructive peripheral arteriopathies are investigated with traditional histology and fluorescent antibody technique. The presence of various pictures of microangiopathy is confirmed, mainly haemodynamic (hyaline-oedematous perivascular thickening) phenomena. Since specific fluorescence is absent from any lesion, the autoimmune pathogenic hypothesis may be discarded.


Selected bibliography on associate degree nursing education education.


Anticoagulants.

Anticoagulants.


Antifungal agents.

Antifungal agents.


Chemotherapy.

Chemotherapy.


Diabetes mellitus.

Diabetes mellitus.


Recurrence risk of common congenital malformations.


Pharmacogenetics.

Pharmacogenetics.


Environmental influences in heritable illness.


Some surgeons and their hand injuries.

Some surgeons and their hand injuries.


Trial of an oral antigen in upper respiratory tract infections in two Bristol schools.


The management of purulent exacerbations of chronic bronchitis. A comparison of minocycline and tetracycline hydrochloride.


Problems in the nutrition of the preruminant calf.


Clinical evaluation of tibric acid (CP 18,524) a new hypolipidemic agent.


The role of language in psychiatric treatment in the French Romantic Age. A note on Dr Laurent Cerise.


The Mother Goose syndrome--a lighthearted look at paediatric literature.


New paths in the molecular orbital approach to solvation of biological molecules.


Leprosy.

Leprosy.


Nonmyelomatous monoclonal immunoglobulinemia.


The clinical significance of IgE.

The clinical significance of IgE.


Correlation between histocompatibility antigens and susceptibility to illness.


Nuclear magnetic resonance in the study of biopolymers.


The physiology of auditory frequency analysis.


Membrane noise.

Membrane noise.


Theoretical formalism for the sliding filament model of contraction of striated muscle. Part I.


Membrane surface charge: discrete and uniform modelling.


Cytoplasmic microtubules and their functions.


Low-temperature luminescence studies of proteins and related molecules.


The regulation of egg yolk protein synthesis by steroid hormones.


Letter: Prostaglandins and catecholamines in gonadotropin secretion. The hypothesized role of PGF in potentiating adrenergic transmission, known to be involved in gonadotropin secretion, was tested in McCormack-Mayer model involving use of 26-day old immature rats primed with 5 iu of PMS and given a single subcutaneous injection of progesterone 24 hours later.  Aspirin was injected into the 3rd ventricle prior to the onset of the critical period on the day of the progesterone injection.  Bilateral injections of aspirin into the anterior hypothalamic area (AHA) during onset of critical period were found to block the facilitatory effect of progesterone.  Simultaneous injection of PGF2a or dopamine into AHA however fully restored ovulation blocked by aspirin.  The results show that prostaglandins and catecholamines (CA) interact to induce adrenergic transmission normally required for gonadotropin secretion.  The study also demonstrates the failure of aspirin in the dose used (60 to 100 mcg) to suppress prostaglandin synthesis in the relevant area of the hypothalamus, e.g., AHA, where CA fibers are known to most likely form synapses with LRF secreting neurons.  This finding partly explains the failure of peripherally administered indomethacin to affect LH secretion in Patrono and Serra's experiment.  Other studies cited suggest possible role of hypothalamic and/or pituitary prostaglandins in gonadotropin secretion.  Subcutaneous administration of indomethacin may be more effective in inhibiting prostaglandin synthesis in the ovary than in the hypothalamus, possibly due to differential sensitivity of these tissues and/or poor penetration of the drug across the blood-brain barrier.


Ultrastructure and function of the spindle apparatus. Microtubules and chromosomes during nuclear division.


Air pollution in smoke-filled rooms.

Air pollution in smoke-filled rooms.


Adaptive responses of the liver to foreign compounds, with special reference to microsomal drug-matabolizing enzymes.


Medical aspects of environmental health.


Computer-based storage and retrieval information systems that can be utilized in health, physical education, and recreation research.


Tolerance of the facilitatory effect of morphine on self-stimulation of the medial forebrain bundle in rats.


Cross immunity induced by ribosomal fraction obtained from Escherichia coli 055 B5.


LH-pituitary responsiveness after administration of low doses of LH-RH in amenorrheic patients.


Cytologic diagnosis of a variety of ovarian papillary cystadenoma which became malignant.


Estimation of the degree of damage in gonadotropin secretion in atypical Sheehan's syndrome by the use of the LH-RH test.


The effect of abnormal human hemoglobin (HbS) on the blood meal size of the hematophagous bug Rhodnius prolixus.


Acquired immunity to murine typhoid induced in mice with fractions of Salmonella typhimurium.


Aspects of pyrimidine biosynthesis of Trypanosoma cruzi.


The pulmonary vessels as a filter for circulating megakaryocytes in rats.


Release of antibody-producing and antigen-binding cells from the spleen during the secondary immune response to sheep erythrocytes.


Surface markers on human B and T lymphocytes. IV. Distribution of surface markers on resting and blast-transformed lymphocytes.


Reduced levels of serum haemolytic complement and renal lesions in ovum-fusion-derived tetraparental mouse chimaeras.


Comparative study of antibody-dependent and direct lymphocyte-mediated cytotoxicity in vitro after alloimmunization in the human. I. Kinetics and the effect of anti-immunoglobulins.


Surgical treatment of non-traumatic lower lip lesions with special reference to the step technique. A follow-up on 149 patients.


Hairy cell leukemia: proliferation of a cell with phagocytic and B-lymphocyte properties.


An objective evaluation of naproxen for the inflammatory reaction in the rheumatoid hand.


Gastrointestinal protein loss in rheumatoid arthritis, studied with 51Cr-chromic chloride and 125I albumin.


Collagen: Its structure and function in normal and pathological connective tissues.


Artificial ventilation with continuous positive airway pressure in the management of ventilatory insufficiency of the newborn.


The Strauss coltage drug program.

The Strauss coltage drug program.


The promise of EPSDT.

The promise of EPSDT.


Experimental occult dirofilariasis in dogs with reference to immunological responses and its relationship to tropical eosinophilia in man.


Erythrocyte ATP levels in mice infected with Plasmodium berghei malaria.


Gastrointestinal function, quinine absorption and parasite response in falciparum malaria.


Recrudescence, poor response or resistance to quinine of falciparum malaria in Thailand.


Intestinal parasites and malaria in the Bada and Gimpu areas of Central Sulawesi, Indonesia.


Comparative clinical trials of niclosamide and tetrachlorethylene in the treatment of Fasciolopsis buski infection.


Preliminary survey for chloroquine resistant malaria in parts of North Sumatra, Indonesia.


Study of the H-2 mutations in mice. Communication II. Recombination analysis of the mutation 504.


A clinical trial of oral dehydroemetine in opisthorchiasis.


Study of mutations of genes regulating nucleoside catabolism in Escherichia coli.


Genetic recombination during conjugation in rec minus-strains of Escherichia coli K-12. Communication III. Synthesis of beta-galactosidase after transfer of lac+-genes into rec+-and rec A-recipients.


Reparation and mutagenesis. Communication II. Photoreactivation of premutational defects and interallelic complementation of UV-induced mutations in locus ad2 of strains of wild and uvs1 yeast.


Genetic instability of radiosensitive mutant of yeast.


The nature of phenotypic reversions of deletion mutants with respect to thymidine-phosphorylase in Escherichia coli.


Genetic analysis of multiple phenotypic changes in the mutant Salmonella typhimurium LT2, produced by a single treatment with nitrosoguanidine.


Memories of Virginia Apgar.

Memories of Virginia Apgar.


Diagnosis and management of renal calculi.


Role of platelet factor 3 in hypercoagulable states.


The diagnosis and prevention of post operative venous thrombosis.


Current developments in thrombolytic therapy.


Use of defibrinating agents ancrod and reptilase in the treatment of thromboembolism.


New approach to thrombolytic therapy: the use of Defibrase in connection with streptokinase.


Interaction of fibrin with various cells.


Coronary circulation time in man.

Coronary circulation time in man.


Changes of specific activity and synthesizing activity of catalase in acatalasemia mouse liver.


Design of bioactive compounds.

Design of bioactive compounds.


Alkylating agents.

Alkylating agents.


Synthetic interferon inducers.

Synthetic interferon inducers.


Antimetabolites: molecular design and mode of action.


Molecular approaches for designing antiviral and antitumor compounds.


Occurrence of amines in the posterior salivary glands of the octopus Hapalochlaena maculosa (cephalopoda).


Double embedding with nitrocellulose and paraffin.


Frank Henry John Figge 1904-1973.

Frank Henry John Figge 1904-1973.


Preparation of fungal hyphae grown on agar-coated microscope slides for electron microscopy.


Paraffin embedding after O.C.T.

Paraffin embedding after O.C.T.


A BEEM capsule chamber-pipette for handling small specimens for electron microscopy.


Letter: Syncytial masses in Panstrongylus megistus naturally infected with Trypanosoma cruzi.


Letter: Trypanosomiasis in rats with trichinosis.


Enhancement of renal allografts in rats and man.


Lymphocyte mediated cytotoxicity in vitro. Induction and inhibition by humoral antibody and nature of effector cells.


Members of the Association of American Physicians.


Joseph Charles Aub 1890-1973.

Joseph Charles Aub 1890-1973.


Emmet Blackburn Bay 1901-1973.

Emmet Blackburn Bay 1901-1973.


Platelet coagulant activities and venous thrombosis after hip surgery.


John Holmes Dingle 1908-1973.

John Holmes Dingle 1908-1973.


Colchicine therapy of familial Mediterranean fever.


William Weborg Engstrom 1915-1974.

William Weborg Engstrom 1915-1974.


Robert Dunning Dripps 1911-1973.

Robert Dunning Dripps 1911-1973.


Robert Frederick Loeb 1895-1973.

Robert Frederick Loeb 1895-1973.


Alexander Benjamin Gutman 1902-1973.

Alexander Benjamin Gutman 1902-1973.


New progressive factors affecting response and survival in adult acute leukemia.


The "phosphate flush": a new index of secretory stimulation in pancreatic islets.


Elliot Voss Newman 1914-1973.

Elliot Voss Newman 1914-1973.


dee. 1886-1973.0dee.

dee. 1886-1973.0dee.


Maurice B. Strauss 1904-1974.

Maurice B. Strauss 1904-1974.


Louis Gordon Welt 1913-1974.

Louis Gordon Welt 1913-1974.


Paul Dudley White 1886-1973.

Paul Dudley White 1886-1973.


Presentation of the George M. Kober medal to Maxwell M. Wintrobe.


The Louis G. Welt Memorial Lecture. Oxygen transport.


Biochemistry of covalently bound flavins.


The fat-soluble vitamins in modern medicine.


The vitamin concept.

The vitamin concept.


Vitamin A transport and retinol-binding protein metabolism.


Aspects of the metabolism of retinol-binding protein and retinol.


Distribution of retinol-binding protein in tissues.


Metabolism of vitamin A and the determination of vitamin A status.


Vitamin A metabolism and requirements in the human studied with the use of labeled retinol.


Synthetic analogs of 1alpha,25-dihydroxyvitamin D3 and their biological activity.


New evidence concerning mechanisms of action of vitamin E and selenium.


Metabolism and properties of a liver precursor to prothrombin.


New concepts relating to the mode of action of vitamin K.


Studies on the absorption and metabolism of phylloquinone (vitamin K1) in man.


Congenital syphilis--a review.

Congenital syphilis--a review.


Editorial: Carl Bemis Hall.

Editorial: Carl Bemis Hall.


The use of the R/B tubes in diagnostic bacteriology.


Birth control in nineteenth-century America: a view from three contemporaries. Social, philosophical and technical aspects of birth control in nineteenth-century America are examined through the lives and thoughts of three men who lived and worked at that time: John Humphrey Noyes, Anthony Comstock and Edward Bliss Foote.