Gene flow and population differentiation. There are many possible spatial patterns of selection and gene flow that can produce a given cline structure; the actual geography of natural selection and gene flow must be worked out before an attempt is made to explain a given natural cline in terms of a model. The results of experimental and theoretical models show that it is possible for local differentiation to evolve parapatrically in spite of considerable gene flow if the selection gradients are relatively uniform. Irregularities in environmental gradients increase the sensitivity of clines to the effects of gene flow in proportion to the increase in the differences in gene frequencies between the emigrants and the demes receiving the immigrants. It is not necessary for a sharp spatial environmental change to be present for distinct differentiation to occur. In some cases even a gentle environmental gradient can give rise to marked spatial differentiation along a genetically continuous series of demes; such environmental differences may be below the practical limits of resolution in field studies. Any asymmetry in gene flow does not lead to dedifferentiation if the environmental gradient is smooth; it merely shifts the position of the transition zone between the differentiated areas from that which would be expected if there were no asymmetry. Abrupt geographic differences in gene, genotype, or morph frequencies should not, therefore, be interpreted as evidence for environmental changes in the immediate vicinity of the steepest part of the cline; neither should they be interpreted as evidence for geographic barriers, sharp environmental differences, or sexual isolation among the differentiated groups of populations when there are no other sources of evidence for these phenomena. Gene flow may be unimportant in the differentiation of populations along environmental gradients.


Development of environmental health criteria for urban planning. Report of a WHO scientific group.


Hepatitis associated antigen (HAA) in urine from serum HAA positive renal transplant recipients.


Leukolytic activity of Acinetobacter calcoaceticus.


The formation of extracellular proteolytic enzymes by Staphylococcus aureus.


Properties of purified phospholipase C from Acinetobacter calcoaceticus.


Different patterns of dissociation of polyribosomes in the presence of EDTA and polyvinylsulfate.


Ultrastructure of congenital pseudarthrosis of the tibia.


Carbon-13 NMR spectroscopy of red blood cell suspensions.


Actions of 2-methyl-1,4-naphthoquinone in Bacillus cereus.


Antiserum-induced adherence of small lymphocytes to heterologous red cells.


Morphogenetic experiments in cleft palate: mandibular response.


Craniospinal dysraphia in the chick embryo produced by tissue-specific antibodies.


Dynamic aspects of cerebrospinal fluid.

Dynamic aspects of cerebrospinal fluid.


Suppression of serum thyrotropin (TSH) by L-dopa in chronic hypothyroidism: interrelationships in the regulation of TSH and prolactin secretion.


Relative insensitivity of rhesus monkeys to the LH-releasing hormone (LH-RH).


Von Recklinghausen's disease with involvement of the rectum: report of a case.


Effect of oleate, diphenylhydantoin and heparin on the binding of  125 I-thyroxine to purified thyroxine-binding globulin.


Prostaglandin F-2  induced steroidogenesis and luteolysis in the primate corpus luteum.


Age differences in retention of concurrent discrimination problems in monkeys.


Osmotic regulation of toad bladder responsiveness to neurohypophyseal hormones. The effect of dilution of the interstitial fluids on the responsiveness of the toad urinary bladder to antidiuretic hormones has been examined in vivo and in vitro. Toads were given periodic injections with vasopressin while in water so that their plasma osmolality fell below 190 mosmoles/kg H(2)O. The hydraulic conductivity of bladders which had been removed from the animal and fixed with 1% glutaraldehyde was 10-fold less in overhydrated toads than in normally hydrated controls. A similar inhibitory phenomenon was observed in in vitro studies, when the tonicity of Ringer's fluid in which the bladders were suspended was lowered from its isotonic value. Mannitol, but not urea, could be effectively substituted for one-half of the NaCl content of Ringer's fluid. In other experiments it has been shown that the responsiveness of the bladder to vasotocin is depressed during bulk water movement across the tissue. This "flux inhibition" was found to depend upon the velocity and the duration of water flow from mucosa to the serosa. It is suggested that the responsiveness of the toad bladder to antidiuretic hormones diminishes as the effective osmotic pressure of the interstitial fluids declines.


Venereal disease among Nigerian women attending intra-uterine contraceptive device clinics.


Serum transferrin levels in chronic renal failure.


The effect of Leucocytozoon smithi infection of production, fertility, and hatchability of Broad Breasted White turkey hens.


Response of broiler chicks to a single dose of aflatoxin.


Turkey leucocytozoon infection. 2. Cryobiologic preservation of Leucocytozoon smithi sporozoites.


Enzymatic hydrolysis of pteroylpolyglutamates in cabbage.


Enzyme induction by polychlorinated biphenyls relative to known inducing agents.


Tuberculin antigens active in human lymphocyte blastogenesis.


Effects of drugs and chemicals on subcellular organelles: mitochondrial contraction and coronary dilators.


Synthesis and biological activity of 7-methyl-8-bromo-10-(1'-D-ribityl)isoalloxazine, an analog of riboflavin.


The histaminase activity of ceruloplasmin.


Selenium: dietary threshold for urinary excretion in the rat.


Recovery of hepatitis agents in the marmoset from human cases occurring in Costa Rica.


Loss of immunologic reactivities of Australia antigen after incubation with bacteria.


Studies on the mode of action of diphtheria toxin. VI. Inhibition of protein synthesis induced by local infection with toxingenic Corynebacterium diphtheriae.


Preparation and characterization of a slime layer material produced by Staphylococcus aureus.


Frozen blood in transplant recipients to avoid hepatitis and-or HL-A sensitization.


Further experience with infection after cardiac transplantation.


Prevention of infections in transplantation patients.


Observations on experimental and clinical liver transplantation.


Bacteria isolated from cutworms and their infectivity to Agrotis spp. (Lepidoptera, Noctuidae).


Alumina--attached spores of Bacillus stearothermophilus for the control of sterilization process.


Extraction and determination of Aspergillus flavus metabolites-aflatoxins from meat products.


Reduction of tetrazolium salts containing heterocyclic compounds by tubercle bacilli.


Effect of isoniazid on the carbohydrate metabolism of isoniazid-susceptible and isoniazid-resistant Mycobacterium tuberculosis H37Rv.


Tuberculous meningitis in children. A review of 25 cases observed between the years 1965 and 1970 at the Kings County Medical Center of Brooklyn with special reference to the problem of infection with primary drug-resistant strains of M. tuberculosis.


Properties of mycobacteriophage DS6A. II. Lipid composition.


Total synthesis of D-aldgarose.

Total synthesis of D-aldgarose.


Unusual oxidation states of metalloporphyrins: octaethylporphinatosilver(3) perchlorate.


Protection of adenosine 5'-triphosphate toward hydrolysis by the formation of a mixed-ligand metal ion complex.


Structure determinations of nitrogen compounds with the aid of NQR, NMR, and ESCA spectroscopy.


The distribution of 5,5-diphenylhydantoin (DPH) and its metabolites in maternal anf fetal Rhesus monkey tissues.


Inhibition of catecholamine oxidation by indoles.


Role of 4-diazoimidazole-5-carboxamide in the action of the antitumor agent 5(4)-(3,3-dimethyl-1-triazeno) imidazole-4-(5)carboxamide in Bacillus subtilis.


Methemoglobin--induced by carcinogenic aminoazo dyes in rats.


Clinical dose-response curves of human malignant epithelial tumours.


Special report no. 7. Thyroid radionuclide uptake measurements. Report of a panel convened by the International Atomic Energy Agency, 1971.


Decrease of ribonuclease activity of isolated rat liver cytoplasmic ribosomes after phenobarbital administration.


A comparative description of the functions of gamma cameras and rectilinear scanners.


Detection of prostaglandin induction of erythrocyte sickling.


Radioassay of serum folate, with use of a serum blank and nondialyzed milk as folate binder.


Serial plasma growth hormone levels in 33 cases of severe diabetic ketoacidosis--effects of sodium nicotinate.


Acid amyloglucosidase and carbohydrate regulation. 3. The induction of sulphonylurea-stimulated insulin release and its dependence on intracellular monoamines.


Influence of synthetic TRH on glucose, free fatty acids and insulin in plasma of healthy persons.


Prolactin releasing effects of TRH in the bovine and their depression by a prolactin inhibitor.


The antibacterial action of canine prostatic fluid and human seminal plasma in an agar diffusion assay system.


A new white cell test which measures individual phagocyte function in a mixed leukocyte population. I. A neutrophil defect in acute myelocytic leukemia.


The effect of thyrotrophic releasing factor on different hypothalamic areas and the anterior pituitary gland: a biochemical and ultrastructural study.


Studies of staphylokinase.

Studies of staphylokinase.


Photodynamic inactivation of rubella virus.


Spread of Pseudomonas aeruginosa in a burns unit.


Physiological rôle of prostaglandins in the control of parturition and menstruation.


On the virus-like particles detected in the biopsy materials (lymphgland) of cancer and leukemic tissue in man.


Epidemiology and virology of leukemia.

Epidemiology and virology of leukemia.


Inheritance of deciduous tooth size in Macaca nemestrina.


Epidermodysplasia verruciformis: its virus and cancer.


Virological approach to human leukemia from murine leukemia.


Restraint of monkeys for craniofacial research.


Nonhuman primate animals for research in gastrointestinal problems.


Experimental Chagas disease in purebred beagle dogs acutely infected with Trypanosoma cruzi (B strain).


Analytical polyacrylamide gel electrophoresis of milligram quantities of RNA.


A simple procedure for study of ribosomal and transfer RNA of Drosophila melanogaster.


A rapid spectrophotometric assay for carnitine palmitoyltransferase.


Extent of plasma protein binding of amphetamine in different species.


The effects of tolbutamide and glibenclamide on intestinal glucose absorption.


Studies on the metabolism of ergoline derivatives. Metabolism of nicergoline in man and in animals.


Small lymphocyte phosphorylation of cytarabine--an organ and species survey.


Hydroxylation of 3,4-benzpyrene in human fetal tissue homogenates.


The nucleic acid-hydroxylapatite interaction. I. Stabilization of native double-stranded deoxyribonucleic acid by hydroxylapatite.


The nucleic acid-hydroxylapatite interaction. II. Phase transitions in the deoxyribonucleic acid-hydroxylapatite system.


Abortive complexes of  -amylases with lanthanides.


Prohistidine decarboxylase from Lactobacillus 30a. A new type of zymogen.


Comparative study of the circular dichroism spectra of reaction centers from several photosynthetic bacteria.


Nucleoside phosphomonoesterases during growth cycle of Bacillus subtilis.


The chemical basis of the virulence of Brucella abortus. X. A surface virulence factor which facilitates intracellular growth of Brucella abortus in bovine phagocytes.


Substrate specificity and kinetic studies on thiogalactoside transacetylase.


Early protection by vaccines against Pseudomonas aeruginosa colonizing burns.


The isolated renal pyramidal infarct: its relationship to calyceal diverticulum and pseudodiverticulum.


Vesico-intestinal fistulae.

Vesico-intestinal fistulae.


A study of Mycobacterium gordonae and Mycobacterium marianum (Scrofulaceum).


A study with 15N-urea on the effects of a low protein diet and malnutrition on urea metabolism in children.


Composition of pteroylpolyglutamates (conjugated folates) in guinea-pig liver and their formation from folic acid.


Automated antibody (anti-D) quantitation technique.


A case of abortion consequent upon infection with Brucella abortus biotype 2. The case described here is that of young primigravida in normal health who, on serological evidence, contracted acute brucellosis, and subsequently aborted spontaneously, Br. abortus biotype 2 being isolated from the amniotic fluid.


Early functional hemodynamic impairment in baboons after 1000 R or less of gamma radiation as revealed by hemorrhagic stress.


X-ray-induced delay of cell division in synchronized Tetrahymena pyriformis.


Carcinoembryonic antigen in urine of cancer patients.


Movement-sensitive neurones in the toad's retina.


Antibody protein levels in the maternal serum in rhesus iso-immunization.


Simultaneous measurement of total exchangeable potassium and sodium using 43 K and 24 Na.


Dose distribution with high voltage irradiation of mediastinal Hodgkin's diseases.


The effect of alteration of thyroxine binding capacity on the dialyzable and absolute fractions of triiodothyronine in circulation.


Accumulation of  137 Cs in Finnish Lapps.


The effect of sorbitol on the microbiology of the dental plaque in monkeys (Macaca irus).


Molecular interactions between the extracellular polysaccharides of Streptococcus mutans.


Changes in periodontal membrane, bone and permanent teeth following experimental pulpal injury in deciduous molar teeth of monkeys (Macaca irus).


Genetic variation of the cytoplasmic and mitochondrial malic enzymes in the monkey Macaca nemestrina.


Ribosomal ribonucleic acid and ribosomal precursor ribonucleic acid in Anacystis nidulans. The RNA of the blue-green alga Anacystis nidulans contains three ribosomal RNA species with molecular weights of 0.56x10(6), 0.9x10(6), and 1.1x10(6) if the RNA is extracted in the absence of Mg(2+). The 0.9x10(6)mol.wt. rRNA is extremely slowly labelled in (32)P-incorporation experiments. This rRNA may be a cleavage product of the 1.1x10(6)mol.wt. rRNA from the ribosomes of cells in certain physiological states (e.g. light-deficiency during growth). The cleavage of the 1.1x10(6)mol.wt. rRNA during the extraction procedure can be prevented by the addition of 10mm-MgCl(2). (32)P-pulse-labelling studies demonstrate the rapid synthesis of two ribosomal precursor RNA species. One precursor RNA migrating slightly slower than the 1.1x10(6)mol.wt. rRNA appears much less stable than the other precursor RNA, which shows the electrophoretic behaviour of the 0.7x10(6)mol.wt. rRNA. Our observations support the close relationship between bacteria and blue-green algae also with respect to rRNA maturation. The conversion of the ribosomal precursor RNA species into 0.56x10(6)- and 1.1x10(6)-mol.wt. rRNA species requires Mg(2+) in the incubation medium.


Membrane proteins of chromaffin granules, dopamine  -hydroxylase, a major constituent. 1. Soluble lysates and membranes were prepared from chromaffin granules isolated from bovine adrenal medulla. The detergent N-cetylpyridinium chloride was used for solubilizing the membrane proteins, including the membrane-bound dopamine (2,4-dihydroxyphenethylamine) beta-hydroxylase. The solubilized proteins were fractionated by Sephadex chromatography in the presence of N-cetylpyridinium chloride. The major component of the membrane proteins, i.e. chromomembrin A, was identified as the enzyme dopamine beta-hydroxylase. 2. The addition of N-cetylpyridinium chloride to the soluble lysate caused precipitation of up to 96% of the proteins, but only a small proportion of the dopamine beta-hydroxylase activity was precipitated. The only protein demonstrable in the supernatant by polyacrylamide-gel electrophoresis was the protein that has a lower mobility than chromogranin A in disc gel electrophoresis. This component has been identified previously as dopamine beta-hydroxylase. Thus, this method provides an extremely simple isolation procedure for dopamine beta-hydroxylase. 3. A comparison of the membrane-bound and soluble dopamine beta-hydroxylases revealed the identity of these two preparations. Both were activated by N-cetylpyridinium chloride, they migrated identically in polyacrylamide-gel electrophoresis, their amino acid composition was very similar and an immunological cross-reaction could be demonstrated.


The graphical determination of K m  and K i . The principle of Dixon (1965) has been extended to give rapid graphical methods for determining enzyme constants for substrates (K(m)) and inhibitors (K(i)). It does away with the sometimes questionable assumption that the amounts of substrate or inhibitor bound by the enzyme are negligible in comparison with the total amount added, and is therefore valid even for cases of high affinity, where the usual methods fail. Besides doing away with the need for calculation, it enables the concentrations of the various components of the system to be read off directly for any point of the velocity curve.


Influence of some hydrosoluble substances with vitamin P activity on the fragility of lysosomes in vitro.


The lysis of paroxysmal nocturnal haemoglobinuria red cells by serum and cobra factor.


Experimental transfusion reactions in monkeys: haemolytic, coagulant and renal effects of transfused isoimmune IgG and IgM.


Tangential excision and grafting of burns. The method, and a report of 50 consecutive cases.


Diet and congenital defects.

Diet and congenital defects.


Severe hyponatraemia in hyperlipaemic diabetic ketosis.


The hinge region of IgG3, an extended part of the molecule.


Chromophore-containing peptide sequences in C-phycocyanin from Mastigocladus laminosus.


Mode of tilorone hydrochloride interaction to DNA and polydeoxyribonucleotides.


Osmotic properties of sonicated mitochondrial fragments.


Protein A from Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation of immunoglobulins.


Purification of the galactose-binding hemagglutinin of Pseudomonas aeruginosa by affinity column chromatography using sepharose.


Amounts of lactoferrin in human colostrum and milk.


Practical effects of blood group incompatibility between mother and fetus.


Thoracic surgery in the laboratory animal.


Application of an intramammary route of vaccination against transmissible gastroenteritis in swine.


Biochemistry of the pteridines.

Biochemistry of the pteridines.


Model reactions for synthesis of pharmacologically active polymers by way of monomeric and polymeric reactive esters.


Bacterial flora of respiratory tract in patients with cystic fibrosis, 1950-71.


Bacterial infection in cystic fibrosis.

Bacterial infection in cystic fibrosis.


Use of 5-fluorocytosine in systemic candidiasis in infancy.


Vitreous surgery. VII. Corneal protector for subtotal vitrectomy.


Nifurtimox: causal treatment of Chagas' disease.


The structure-activity relationship of 4-(5'-nitrofurfurylidene-amino)-tetrahydro-4H-1,4-thiazine-1,1-dioxides active against Trypanosoma cruzi.


Animal experimental investigation into the activity of nifurtimox against Trypanosoma cruzi.


Investigations on the pharmacokinetics of nifurtimox- 35 S in the rat and dog.


The effect of nifurtimox in acute Chagas' infection.


Experience with nifurtimox in chronic Chagas' infection. Preliminary report.


DNA replication in a polymerase I deficient mutant and the identification of DNA polymerases II and 3 in Bacillus subtilis.


Anti-inflammatory and pharmacokinetic properties of sudoxicam N-(2-thiazolyl)-4-hydroxy-2-methyl-2H-1,2-benzothiazine-3-carboxamide 1,1-dioxide.


Fluorometric study of tetracycline--bovine serum albumin interaction. The tetracyclines--a new class of fluorescent probes.


Single bolus injection or slow infusion for intravenous cholangiography? Measurement of iodipamide (Biligrafin) excretion using a rhesus monkey model.


Brain swelling due to experimental cerebral infarction. Changes in vasomotor capacitance and effects of intravenous glycerol.


Steady-state kinetics of enzyme reactions in the presence of added nucleophiles.


Cancer statistics 1973.

Cancer statistics 1973.


Parameter evaluation in second-degree rate equations for enzyme-catalyzed reactions.


The etiology and pathogenesis of oral cancer.


Needle aspiration of breast cysts.

Needle aspiration of breast cysts.


Protein synthesis in mitochondria of Euglena gracilis.


N-acetylglutamate synthetase of Pseudomonas aeruginosa. An assay in vitro and feedback inhibition by arginine.


Transplantable immunoglobulin-secreting tumours in rats. Purification and chemical characterization of four kappa chains from LOU-Wsl rats.


Temperature-sensitive mutants affected in DNA synthesis in phage phi29 of Bacillus subtilis.


Classics in oncology. Excerpts from: On some morbid appearances of the absorbent glands and spleen, Thomas Hodgkin.


Regulation of the catabolic ornithine carbamoyltransferase of Pseudomonas fluorescens. A study of the quaternary structure.


Thomas Hodgkin (1798-1866).

Thomas Hodgkin (1798-1866).


The National Cancer Program Plan.

The National Cancer Program Plan.


An alternative to removal of an infected pacemaker.


The soluble proteins of the bovine cornea.


Reticuloendothelial phagocytosis of Cr 51 -labeled sheep red cells in mice. I. Assay employing total body counting.


The role of the private sector in cancer control.


Serological relatedness of bacterial deoxyribonucleic acid polymerases. A number of bacterial species have been surveyed for serological activities with antiserum to Escherichia coli B deoxyribonucleic acid (DNA) polymerase I (EC 2.7.7.7.). The degree of serological cross-reaction is taken as a measure of relatedness of both the enzyme molecules from various species and the bacterial species themselves. Extracts were assayed by complement fixation only after treatment with deoxyribonuclease, since DNA bound to DNA polymerase alters the serological activity of the enzyme. Antiserum to E. coli DNA polymerase I did not react with either purified E. coli DNA polymerase II or the phage T4-induced DNA polymerase.


Serological study of nocardia by using mycobacterial precipitation reference systems. Forty-six nocardia strains and ten strains labeled "Mycobacterium" were analyzed with the comparative immunodiffusion technique, employing as reference material nine mycobacterial precipitation systems, each one representing a different species. Three precipitinogens were common for many of the tested strains and the mycobacterial reference strains. The serological analyses indicated the possibility of differentiating two groups within Nocardia depending on the characteristics of one of the common precipitinogens. Five of eight tested Nocardia farcinica strains showed a serologically close relationship with the mycobacteria and might be regarded as belonging to this genus. The "M." pellegrino and "M." rhodochrous strains, however, did not react with the antimycobacterial sera to a higher degree than did the tested nocardia strains.


Differentiation of some enterococci by gas chromatography. Relative fatty acid compositions of 37 enterococci were examined by gas chromatography. Streptococcus faecalis, S. faecium, and S. faecium var. durans yielded similar fatty acid patterns. Strains of S. faecium var. casseliflavus, and a motile yellow-pigmented streptococcus, contained very low levels of C(19:0) cyclopropane fatty acid and four unidentified components, compared to the other strains of enterococci examined. There were no significant differences in the fatty acid patterns of enterococci grouped according to plant, animal, or human source.


Genetic analysis of repair of ultraviolet damage by competent and noncompetent cells of Bacillus subtilis. The repair of ultraviolet (UV) damage in Bacillus subtilis W23T(-) has been studied by transformation with deoxyribonucleic acid (DNA) extracted from irradiated cells before and after repair. The extent of repair of genetic markers by donor cells after low or moderate doses of UV was found to be related only to the initial degree of inactivation. After a very high dose, further inactivation occurred, also in proportion to initial damage. In addition, the competent recipient cells were shown to repair approximately 75% of the damage in transforming DNA. The sensitivities of markers irradiated either in vivo or in vitro appeared to be related to map position, the more proximal markers showing a greater resistance to UV inactivation.


Phosphorylase activity in liver and muscle tissue in protein calorie malnutrition.


Oligopeptidases of brush border membranes of rat small intestinal mucosal cells. 1. The properties of peptidases located in the brush borders of rat small intestinal mucosal cells have been investigated using a new method for the assay of peptidase activity. In this method amino acids produced by hydrolysis of peptides are oxidized by ophidian L-amino acid oxidase and the hydrogen peroxide produced during reoxidation of the reduced enzyme is estimated spectrophotometrically.2. 10-20% of the total cellular peptidase activity and 50% of the leucylnaphthylamidase (LNAase) activity were found to be tightly bound to the brush borders and to possess different substrate specificity from the supernatant peptidase activity.3. Evidence from kinetic and competition studies indicates the presence of more than one peptidase in the brush borders. The peptidases exhibit pH optima of 8.0-8.5, are inhibited by EDTA, but are not usually activated by divalent metal ions. The brush border peptidases hydrolyse di- and tripeptides, some oligopeptides, leucinamide and leucyl-beta-naphthylamine. On the basis of the Michaelis constants, these substrates differ in their affinities for the enzymes.4. It is proposed that the brush border peptidases serve an analogous function in the terminal stages of protein digestion to that of the disaccharidases in the case of carbohydrate digestion and absorption.


Meningococcal disease and its control.

Meningococcal disease and its control.


Parenteral alimentation of premature infants under 1,200 grams.


Fine structure of frozen-etched spores of Thelohania bracteata emphasizing the formation of the polaroplast.


Infected oral lesions of cyclic neutropenia.


The termination of optic fibres in the lateral geniculate nucleus of some primates. An ultrastructural study.


Lipofuscin in monkey lateral geniculate body. An electron microscope study.


Multiple myeloma without initial paraproteins.


Supervised patient-management of hemophilia. A study of 45 patients with hemophilia A and B.


Cirrhosis associated with partial deficiency of alpha-1 antitrypsin in an adult.


Nutrition in the Third World.

Nutrition in the Third World.


Cost-benefit of intensive care.

Cost-benefit of intensive care.


Mass in tuberculous peritonitis.

Mass in tuberculous peritonitis.


Measles virus: induced transfer of biological properties.


Red-cell metabolism of pyridoxine in sideroblastic anaemias and related anaemias.


Modification of dominance by selection in the homozygote.


Spontaneous mutations in Drosophila melanogaster.


Mutation mechanisms in Drosophila.

Mutation mechanisms in Drosophila.


Serum oxytetracycline concentrations after intravenous and intramuscular administration in horses.


S survey of ocular findings in 16- to 24-week-old beagles.


Distribution of soluble proteins in the mammalian neurohypophysis and their cross-species reactivity with anti-neurophysin.


The tentative identification of three neurophysins from the rat posterior pituitary gland.


The effect of competence regime on competence, DNA absorption and integration of DNA in cultures of Bacillus subtilis.


The nutrition of Bacillus megaterium and Bacillus cereus.


End-products, fermentation balances and molar growth yields of homofermentative lactobacilli.


DNA competition studies within the Bacillus cereus group of bacilli.


Surface changes in a strain of Rhizobium trifolii on mutation to bacteriophage resistance.


Evolutionary mechanism of adaptation of Arthrobacter histodinolovorans and Pseudomonas aeruginosa to use L-histidinol as a sole source of nitrogen and carbon.


Influence of culture pH on the content and composition of teichoic acids in the walls of Bacillus subtilis.


Separation of two distinct tumor-associated antibodies in the serum of melanoma patients.


Acetylation of dideoxykanamycin B by Pseudomonas aeruginosa.


Tolerance induction against a human kappa I -L-chain with subsequent immunization against kappa 3 -L-chain in rabbits.


The influence of temperature and sex on the amino acid requirements of the broiler.


Immunologic response of turkeys to an avirulent Pasteurella multocida vaccine in the drinking water. 2. Duration of immunity.


Gold retention and subsequent mortality in strains of chickens resistant and susceptible to lymphoid leukosis or Marek's disease.


Effect of disodium cromoglycate (Intal) on sputum protein composition.


A case of pyometritis in an East African de Brazza's monkey (Cercopithecus neglectus).


Splenomegaly and red blood cell destruction: a morphometric study on the human spleen.


The ultrastructure of the lymphoreticular cells in non-lymphoid human neoplasms.


Endothelial contraction in vivo: a study of the rat mesentery.


The use of primates in research and its effect on conservation.


Studies on bovine trypanosomiasis. Serum immunoglobulin levels in Zebu cattle exposed to natural infection in East Africa.


A study of the cell lines required to detect a variety of veterinary viruses in routine diagnostic conditions.


The serological response to Aspergillus fumigatus antigens in bovine mycotic abortion.


Short-gut syndrome: a review with a case report.


Localization of carbonic anhydrase activity in the vertebrate retina.


The effect of biguanides on secretion and biosynthesis of insulin in isolated pancreatic islets of rats.


Ocular and optic nerve blood flow at normal and increased intraocular pressures in monkeys (Macaca irus): a study with radioactively labelled microspheres including flow determinations in brain and some other tissues.


The effects of pilocarpine and neostigmine on the blood flow through the anterior uvea in monkeys. A study with radioactively labelled microspheres.


Regulation of ribosomal RNA and 5s RNA synthesis in Drosophila melanogaster. I. Bobbed mutants. Analysis of the rates and amounts of rRNA and 5s RNA synthesized in Drosophila melanogaster bobbed mutants was done by using acrylamide-gel electrophoresis. The results show that the amounts of rRNA synthesized are constant, although the rates of rRNA synthesis in bb's are reduced to 30% of the wild-type level. The rates of synthesis of 5s RNA were constant. The rate of synthesis of the two kinds of molecules that enter in equimolar amounts into the mature ribosome is non-coordinated.-The rates of rRNA synthesis were shown to be proportional to the length of the scutellar bristles, supporting the notion that in trichogen cells there is no developmental delay, but the size of the bristle depends directly on the rate of rRNA synthesis.


The relative influence of sex of progeny on the lethal expression of the sonless gene in Drosophila melanogaster. The sex-linked maternally influenced lethal gene, sonless (snl), in D. melanogaster was shown to affect the survival of daughters as well as sons of snl/snl mothers but to a lesser degree. Interaction studies of sonless with the sex altering mutants transformer (tra) and doublesex (dsx) revealed that any alteration toward increased masculinity of progeny from snl/snl females reduced their zygotic viability, independent of snl dosage or Y chromosome presence or absence. Possible implications of these results are discussed and the original gene action hypothesis for snl is extended to incorporate these new findings.-Sonless is non-allelic but closely linked to rudimentary (r) and fused (fu), two mutants with similar sex-ratio patterns.


The effect of oxygen on the frequency of somatic recombination in Drosophila melanogaster. The influence of oxygen on the frequency of somatic recombination in the yellow singed system on the X chromosome of Drosophila melanogaster was studied under a variety of experimental conditions. Flies raised from egg to adult in atmospheres containing 70-90% oxygen were found to have significantly more mosaic spots on their abdominal tergites than were observed in flies which developed in air. First instar larvae X-rayed in from 0 to 100% oxygen demonstrated the existence of an oxygen effect for somatic recombination in the cells which form the abdominal hypoderm. The mosaic spot counts, beginning with the lowest numbers which were found in flies X-rayed in nitrogen, increased rapidly with rising oxygen tensions until the percentage in air was reached, then leveled off at the higher concentrations. Post-treatment with nitrogen of larvae X-rayed in air or oxygen created a substantially higher number of mosaic spots than were found when larvae, after being similarly irradiated, were instead placed into air or oxygen.


Sex chromosome translocations in the evolution of reproductive isolation. Haldane's rule states that in organisms with differentiated sex chromosomes, hybrid sterility or inviability is generally expressed more frequently in the heterogametic sex. This observation has been variously explained as due to either genic or chromosomal imbalance. The fixation probabilities and mean times to fixation of sex-chromosome translocations of the type necessary to explain Haldane's rule on the basis of chromosomal imbalance have been estimated in small populations of Drosophila melanogaster. The fixation probability of an X chromosome carrying the long arm of the Y(X.Y(L)) is approximately 30% greater than expected under the assumption of no selection. No fitness differences associated with the attached Y(L) segment were detected. The fixation probability of a deficient Y chromosome is 300% greater than expected when the X chromosome contains the deleted portion of the Y. It is suggested that sex-chromosome translocations may play a role in the establishment of reproductive isolation.


Mutation rate and dominance of genes affecting viability in Drosophila melanogaster. Spontaneous mutations were allowed to accumulate in a second chromosome that was transmitted only through heterozygous males for 40 generations. At 10-generation intervals the chromosomes were assayed for homozygous effects of the accumulated mutants. From the regression of homozygous viability on the number of generations of mutant accumulation and from the increase in genetic variance between replicate chromosomes it is possible to estimate the mutation rate and average effect of the individual mutants. Lethal mutations arose at a rate of 0.0060 per chromosome per generation. The mutants having small effects on viability are estimated to arise with a frequency at least 10 times as high as lethals, more likely 20 times as high, and possibly many more times as high if there is a large class of very nearly neutral mutations.-The dominance of such mutants was measured for chromosomes extracted from a natural population. This was determined from the regression of heterozygous viability on that of the sum of the two constituent homozygotes. The average dominance for minor viability genes in an equilibrium population was estimated to be 0.21. This is lower than the value for new mutants, as expected since those with the greatest heterozygous effect are most quickly eliminated from the population. That these mutants have a disproportionately large heterozygous effect on total fitness (as well as on the viability component thereof) is shown by the low ratio of the genetic load in equilibrium homozygotes to that of new mutant homozygotes.


Dosage of the maternal effect gene associated with the tumorous-head abnormality in Drosophila melanogaster. The penetrance of tuh-3 observed in the progeny of triploids with two doses of tuh-1 was not statistically different from that occurring in the progeny of their diploid sisters with two doses. A higher penetrance was observed in the progeny of triploids with three doses of tuh-1 than in the progeny of their diploid sisters with two doses. These observations suggest that the maternal effect responsible for increasing the penetrance of tuh-3, the gene causing the tumorous-head abnormality, is caused by a specific gene product of tuh-1, the maternal-effect allele. In addition there is probably a maternal-effect threshold, lying between amounts produced by one and two doses of tuh-1, below which no increased penetrance of tuh-3 is observed.


The labelled inhibitor method in cytoenzymology.


Follow-up of a family group suffering from ichthyosis hystrix type Curth-Macklin.


Cryomycin, a new peptide antibiotic produced only at low temperature.


Mode of action of tomaymycin.

Mode of action of tomaymycin.


Renal distribution volumes of indocyanine green, ( 51 Cr)EDTA, and  24 Na in man during acute renal failure after shock. Implications for the pathogenesis of anuria. The mechanism responsible for the anuria in acute renal failure after shock is still controversial. Suppressed glomerular filtration and/or tubular back-diffusion of the filtrate are major possible causes. In the present investigation, seven patients with acute anuria, three of these seven again in the polyuric phase, six patients with moderate renal impairment, four patients with chronic renal failure, and eight subjects with normal renal function were studied by a multiple indicator-dilution method in which the total renal blood flow and renal distribution volumes of indocyanine green, [(51)Cr]EDTA, and (24)Na were determined. In normal subjects the average values for one kidney were 582 ml/min, 42 ml, 92 ml, and 139 ml, respectively. The measurements in the patients with moderate renal impairment were similar to those in the normal subjects, but were decreased in chronic renal failure. In acute anuria, the average values were 269 ml/min, 40 ml, 101 ml, and 114 ml and the kidney volume, estimated radiographically, was increased by 40%. When expressed as milliliters per milliliters kidney, the average distribution volume of (24)Na was decreased from 0.64 to 0.38. This decrease is consistent with the hypothesis that suppressed filtration is largely responsible for the anuria and that back-diffusion is, at most, a contributory factor. The apparent contradiction between the relatively well-preserved total blood flow and the suppressed filtration may be due to a combination of afferent vasoconstriction and efferent vasodilatation. This view is supported by the observation that low filtration fractions were found in clearance measurements performed during the polyuric phase.


Some aspects of trachoma control and provisional estimates of the cost of vaccine production.


Clinical aspects of keratomalacia.

Clinical aspects of keratomalacia.


Diphtheria in Iran.

Diphtheria in Iran. A study of diphtheria in Iran was undertaken during the summer of 1969. Diphtheria was found to be more common among the younger people and to reach its peak 3 months after the start of the school term, during the coldest period of the year. Owing to the liberal use of antibiotics a number of modified clinical cases were observed. Of the strains isolated, 89.7% were gravis, 1.3% intermedius and 9% mitis. Phage typing of these strains showed that the mitis and intermedius strains could only be typed by adapted phages and the majority of gravis strains were phage type XIV, which is the epidemic type seen in other countries.As the mass immunization campaign reduces the incidence of diphtheria in Iran, the epidemiological pattern of the disease will change as seen in the U.K. and U.S.A. It is, therefore, suggested that in the future further studies of diphtheria in Iran be undertaken in order to provide us with information about the changing epidemiological pattern of the disease.


Prevention of blindness due to hypovitaminosis A.


Escherichia coli bacteremia in the squirrel monkey. I. Effect of cobra venom factor treatment. Squirrel monkeys were significantly depleted of complement by a nontoxic protein constituent of cobra venom, and the influence of cobra factor (CoF) treatment on the course of Escherichia coli bacteremia was studied. Striking neutropenia occurred rapidly in control animals while the rate of occurrence of neutropenia was 20 to 30 times slower in the CoF-treated animals, suggesting that the E. coli-induced neutropenia was at least partially a complement-mediated response. In the CoF-treated monkeys, the initial rate of clearance of the E. coli from the circulation tended to be slower and the resultant levels of bacteremia were higher than in control animals. These observations are consistent with a hypothesis that complement-mediated neutrophilic leucocyte function is an important host defense mechanism in gram-ngeative bacillary bacteremia.


Serological detection of enterotoxin in foods implicated in staphylococcal food poisoning. Two methods are described for the extraction of enterotoxin from foods incriminated in incidents of staphylococcal food poisoning. Enterotoxin was detected serologically in 12 of 24 food samples from 20 separate incidents: eight samples contained enterotoxin A, three contained D and one both A and B. The amount of enterotoxin in nine foods, based on 100% recovery, varied from 0.02 to 0.09 mug./g.Data are also given on the numbers of Staphylococcus aureus isolated from samples of food from 39 food poisoning incidents. Colony counts varied between 7.5 x 10(5) and 9 x 10(9)/g. with a median value of 7 x 10(7)/g.


Pathologic features of Herpesvirus ateles lymphoma in cotton-topped marmosets (Saguinus oedipus).


Isolation of a virus from cattle with persistent lymphocytosis.


Identification of the immunoglobulin class active in the Rose Bengal plate test for bovine brucellosis. The antibodies active in the Rose Bengal plate test (RBPT) for bovine brucellosis have been studied. The results of fractionation experiments showed that RBPT activity was associated with fractions containing immunoglobulin of the IgG(1) class; other immunoglobulin classes were inactive in this respect although active in other tests. These results were confirmed by inhibition tests with specific antisera and by elution of the antibody from agglutinated RBPT antigen.The major proportion of the serum complement-fixing activity was also present in the IgG(1) fraction and it is suggested that the RBPT and CF reactions are probably mediated by the same antibodies.


Medical treatment of herpes.

Medical treatment of herpes.


Pathogenesis of salmonellosis. Studies of fluid secretion, mucosal invasion, and morphologic reaction in the rabbit ileum. Strains of Salmonella typhimurium were studied in the ligated rabbit ileal loop model to gain insight into the mechanisms whereby bacteria which invade the gastrointestinal mucosa evoke fluid exsorption. The organisms employed differed in various biologic attributes including the ability to invade the ileal epithelium, multiply within the mucosa, elicit an acute inflammatory reaction, and disseminate across the intestinal wall. Some strains provoked small intestinal fluid exsorption although these did not elaborate enterotoxin. Only those strains which invaded the mucosa were accompanied by either mucosal inflammation or fluid exsorption. Noninvasive strains produced neither histologic abnormalities nor fluid secretion. While strains which invaded the mucosa caused an acute inflammatory reaction, not all such strains evoked fluid secretion. Furthermore, there was no correlation in ability of invasive organisms to evoke fluid secretion or in the intensity of mucosal inflammation, number of intramucosal salmonellae, or in ability to disseminate from the rabbit ileum. These observations suggest that, as is the case in shigellosis, mucosal invasion may be a necessary factor for the intestinal fluid loss in salmonellosis. A bacterial property or factor, in addition to invasion of the gastrointestinal mucosa, seems to be responsible for fluid exsorptin. However, it is unlikely that a salmonella enterotoxin comparable to that elaborated by Vibrio cholerae, toxigenic Escherichia coli, or Shigella dysenteriae 1 is related to fluid secretion in salmonellosis.


Experimental amblyopia.

Experimental amblyopia.


Measurement of retinal adhesion.

Measurement of retinal adhesion.


Cellular immune response of humans to pigskin.


Inhibition of a DNA polymerase from Bacillus subtilis by hydroxyphenylazopyrimidines. The antimicrobial agent, 6-(p-hydroxyphenylazo)-uracil, specifically inhibits DNA polymerase III of Bacillus subtilis with a K(i) of less than 1 muM. The inhibition requires prior reduction of the drug, is reversible, and is competitive with dGTP. High amounts of dATP prevent inhibition by the closely related drug, 6-(p-hydroxyphenylazo)-isocytosine. A model is presented in which the inhibitors base-pair with the template while part of a ternary complex with the enzyme. These results imply that DNA polymerase III of B. subtilis is necessary for chromosomal replication.


Genetic transformation in Escherichia coli K12. An auxotrophic strain of E. coli K12 treated with CaCl(2) was transformed for several markers at a frequency of up to 10(-6) per recipient cell by a DNA preparation isolated from a prototrophic strain. The transforming activity of the DNA preparation was eliminated by treatment with DNase, heat, or sonication, whereas RNase or Pronase treatment had little effect. Two closely linked genetic markers (leu and ara) showed a high degree of cotransformation linkage when high molecular weight DNA was used, but the linkage was almost completely eliminated when sheared, smaller molecular weight DNA was used. There is genetic evidence that the transformation is a result of the replacement of the preexisting genetic marker on the chromosome by that of the donor DNA.


Hyperalimentation in a community children's hospital. An eighteen month experience.


Local antibodies to trachoma agent.

Local antibodies to trachoma agent.


Endometrial adenocarcinoma with cerebral metastasis and subdural ossification.


Reverse isolation in bone marrow transplantation: ultra-clean room compared with laminar flow technique. II. Microbiological and clinical results.


The sucessive morphology of virus particles in a case of hepatitis with Australia antigen.


Isolation and metabolism of male sex-dependent urinary protein from rats.


Inappropriate lactation and amenorrhoea: pathological and diagnostic considerations.


Platelet abnormalities in heritable disorders of connective tissue.


Cyclopia.

Cyclopia.


Effects of cold acclimation, hibernation and temperature on succinoxidase activity of heart homogenates from hamster, rat and squirrel monkey.


Isoelectric variants of Macaca irus salivary alpha-amylase.


X-ray inactivation of ribonuclease in the presence of EDTA.


Requirement for calcium ions in lymphocyte transformation stimulated by phytohemagglutinin.


Effect of AMP and related compounds on glycogen content of Tetrahymena.


Studies in myasthenia gravis: active antigenic sites related to humoral antibody induction.


Differentiated functions expressed by cultured mouse lymphoma cells. II. Theta antigen, surface immunoglobulin and a receptor for antibody on cells of a thymoma cell line.


Suppression of the in vitro anamnestic response of rabbit lymph node cells by anti-IgG serum.


Pseudoimmune precipitation by the isolated byssinosis "antigen".


Natural antiglobulin antibodies in primate sera.


Chicken  A: physicochemical and immunochemical characteristics.


The relationship between sedimentation value and immunogenic acitivity of mycobacterial ribonucleic acid.


Greater sensitivity to inhibition by anti-immunoglobulin of splenic than of bone marrow B-lymphocytes.


Giant cemento-ossifying fibroma of the maxilla: report of case and discussion.


Vasectomy: long-term effects in the rhesus monkey.


Acid-base problems after intravenous amino acids.


Cardiocirculatory adaptation to chronic hypoxia. 3. Comparative study of cardiac output, pulmonary and systemic circulation between sea level and high altitude residents.


Recovery of haemopoiesis in lethally irradiated monkeys by frozen allogeneic bone marrow grafts.


Development and use of a children plastic isolation system for prevention of infection.


A blood-aqueous barrier to small molecules in the ciliary processes of the vervet monkey (Cercopithecus aethiops).


Pentose phosphate pathway of erythrocytes in uremia.


Studies on vitelline cells of monogenea. 3. Nature of phenolic material and a possible alternate mechanism involved in hardening of egg-shell in helminths.


Pyrrolidonyl peptidase in some avian and rodent tissues. Histochemical localization and biochemical studies.


Pseudomonas aeruginosa: pyocine types and clinical experience with infections in a general hospital.


An experimental model for the assessment of the effects of cigarette smoke inhalation on pulmonary physiology.


Tuberculous pleurisy.

Tuberculous pleurisy.


The nutritional status of pre-school children in Kenya.


Babesia argentina, Plasmodium vivax and P. falciparum: antigenic cross-reactions.


Protein metabolism. 2. Protein turnover, synthesis, and muscle growth in suckling, young, and adult mammals infected with nematospiroides dubius or Trichostrongylus colubriformis.


Interaction of sporangiomycin with the bacterial ribosome.


Prevalence of Pseudomonas aeruginosa strains possessing R factor in a hospital.


Synthesis of 3',4'-dideoxy and 3',4',5''-trideoxyribostamycin active against kanamycin-resistant E. coli and P. aeruginosa.


Biochemical and microbiological studies on 6-substituted penicillins.


Changes in drinking and feeding habits of mice with experimental scrapie.


A rapid histochemical test for mammalian tyrosinase.


Mortality trends of epidemic meningitis in northern areas of Nigeria, 1961-1970.


Lymphangiography in prostatic carcinoma. Preliminary observations.


Skiing injuries.

Skiing injuries.


Diagnostic potential of amyl nitrite and nitroglycerin.


Von Recklinghausen's disease with multiple meningiomas.


Protected environments and prophylactic antibiotics. A prospective controlled study of their utility in the therapy of acute leukemia.


Thyroid function assessment.

Thyroid function assessment.


Tremor and involuntary movements in monkeys: effect of L-dopa and of a dopamine receptor stimulating agent. Either L-dopa, in combination with 1-alpha-methyldopa hydrazine (MK-486), or 1-(2''-pyrimidyl)-4-piperonylpiperazine, an agent that stimulates dopamine receptors, relieves surgically induced tremor in monkeys and concomitantly evokes involuntary movements. These results indicate that tremor and involuntary movements are associated with a common mechanism and that the activity of the dopamine receptors is involved in the regulation of these dysfunctions.


Phospholipase A activity in rat lung.

Phospholipase A activity in rat lung.


Conditioned inhibition in successive descrimination learning.


Retinal repair after experimental xenon arc photocoagulation. I. A comparison between rhesus monkey and rabbit.


Deep mycoses in India. A critical review.


The relationship of optic nerve tissue pressure to intracranial and systemic arterial pressure.


Provocation of schizophrenic symptoms by intravenous administration of methylphenidate.


Photosynthetic carbon dioxide assimilation by Rhodospirillum rubrum.


Effects of ethylenediaminetetraacetate and diethylenetriaminepentaacetate on DNA synthesis in kidney and intestinal mucosa of folate treated rats.


Iron metabolism after renal transplantation.


Studies on the nature and excretion of biliary copper in man.


Focal seizures--a false lateralizing sign.


Petit mal status without impairment of consciousness.


Fiscal viability in ECF program.

Fiscal viability in ECF program.


Postindustrial Europe and its health care: views of an insider.


The politics of universal health insurance in the United States: an interpretation.


Isolation and characterization of lividomycin A inactivated by Pseudomonas aeruginosa and Escherichia coli carrying R factor.


Characterization of spheroplast membranes of Neisseria meningitidis group B. Spheroplast membranes (spheroplast envelopes) of strain 2091 of group B Neisseria meningitidis were prepared by a procedure that included lysozyme treatment of the cells and osmotic lysis of the resulting spheroplasts. Electron microscopy revealed that the membranes consisted of two unit layers, generally parallel to each other. The membrane preparation migrated as a single component in a 40 to 70% sucrose gradient and consisted of 62% protein, 28% lipid, 9% ribonucleic acid, small amounts of carbohydrate, hexosamine, and deoxyribonucleic acid. When 1 or 10 mug (dry weight) was injected intravenously into rabbits, a mild pyrogenic reaction was elicited. In immunodiffusion tests, immune rabbit serum prepared against spheroplast membranes produced three major precipitin lines, with the homologous antigen solubilized with sodium dodecyl sulfate, and a single line with untreated antigen. The immune serum also reacted with a cell wall antigen, and to a lesser extent with some of the cytoplasmic antigens. Succinate dehydrogenase and reduced nicotinamide adenine dinucleotide (NADH) oxidase activities were found to be associated with the spheroplast membranes. NADH dehydrogenase also was associated with the membranes but was gradually released and recovered in other fractions. Glutamate-oxaloacetate transaminase, glutamate, glucose-6-phosphate, and isocitrate dehydrogenase activities were not found in the membrane preparation. About one-third of these enzymatic activities were recovered in the supernatant fluid after the sedimentation of the spheroplasts and two-thirds were recovered in the cytoplasmic fraction. N-acetylneuraminic acid (NAN)-condensing enzyme and cytidine monophosphate-NAN synthesizing enzyme also were identified in this organism. These enzymes were not associated with the membranes and were recovered from extracts from whole cells, spheroplasts, or cells exposed to osmotic shock, as well as from spheroplast supernatant and shock fluids. It is concluded that the spheroplast membranes of the strain of meningococci used in these studies are typical of those recovered from gram-negative bacteria.


6'-N-acetylation of 3',4'-dideoxykanamycin B by an enzyme in a resistant strain of Pseudomonas aeruginosa.


Studies on nucleolar RNA synthesis in Drosophila melanogaster. I. The relationship between number of nucleolar organizers and rate of synthesis.


Studies on nucleolar RNA synthesis in Drosophila melanogaster. II. The influence of conditions resulting in a bobbed phenotype on rate of synthesis and secondary constriction formation.


Limpet haemocytes. I. Studies on aggregation and spike formation.


Cryptococcal and other forms of mycotic meningitis.


Incidence of haemagglutinating antibodies to meningococci in north-west England. USING AN INDIRECT HAEMAGGLUTINATION TECHNIQUE THE INCIDENCE OF MENINGOCOCCAL ANTIBODIES IN ADULTS WAS FOUND TO BE: group A 21%, group B 3%, and group C 20%. The reasons for some differences between these results and those obtained in America are discussed. A low incidence of antibody was detected in preschool children but the incidence in school children was found to approach that of adults. Comparison of the antibody incidence in the adult population of an area where clinical infections were occurring with that in a similar population elsewhere failed to detect any significant difference.


Maternal protein deprivation during pregnancy or lactation in rats and the efficiency of food and nitrogen utilization of the progeny.


Relation between adrenal cortex and hepatic protein synthesis in protein-calorie-deficient rats.


Bicycle spoke fractures.

Bicycle spoke fractures.


The management of lye burns of the esophagus.


Treatment of lead poisoning.

Treatment of lead poisoning.


Bacid, lactinex and yogurt.

Bacid, lactinex and yogurt.


Viaflex containers.

Viaflex containers.


Total intravenous feeding.

Total intravenous feeding.


Clorazepate (tranxene). Another benzodiazepine.


Genetic hazards of ionizing radiations: cytogenetic extrapolations from mouse to man.


Experimental modification of the "tertiary structure" of chromosomes in human lymphocytes: the effects of polyanionic substances and hypertonic salt solutions.


Comparison of complement--fixation and immunodiffusion tests for the detection of hepatitis-associated antigen.


Intracellular antigens of Streptococcus pyogenes. Isolation by means of column chromatography.


Clinical evaluation of patients treated for rhabdomyosarcoma.


Mood changes during three conditions of alcohol intake.


Unusual causes of small-bowel pneumatosis: perforated duodenal ulcer and perforated jejunal diverticula.


Intra-arterial valium: its safety and effectiveness.


Definitive radiation therapy of carcinoma of the prostate. A report on 15 years of experience.


The effects of malnutrition on the development of cognitive functions of the nervous system in childhood.


Pneumocystis carinii in hares, Lepus europaeus Pallas, in the Netherlands.


Biological memory.

Biological memory.


Human carbonic anhydrases. IX. Inheritance of variant erythrocyte forms.


The distribution of carnitine in male reproductive tissues and its effect on palmitate oxidation by spermatozoal particles.


Generalized Trichophyton mentagrophytes ringworm in Capuchin monkeys (Cebus nigrivitatus).


Abnormalities in lymphocyte proliferation in classical and atypical measles infection.


Studies of delayed hypersensitivity to L. Monocytogenes in mice: nature of cells involved in passive transfers.


Antigen-binding lymphocytes in children with immune deficiency.


Presence of C1q-reactive immune complexes in patients with leprosy.


Passive protective properties of serum fractions from mice inoculated with an anti-pseudomonas vaccine.


Clinical evaluation of serum immunoglobulins in amoebiasis.


Amino acid compositions and N-terminal amino acid of all the tryptic peptides from the   and   polypeptide chains of adult hemoglobin of the Japanese monkey (Macaca fuscata fuscata). Biochemical studies on hemoglobins and myoglobins. IX.


Structures of branched dextrins produced by saccharifying  -amylase of Bacillus subtilis.


A factor stimulating the transcription of Drosophila RNA polymerase.


The mechanism of the adjuvant effect of a tubercle bacillary lipid on the antibody response of rabbits to sheep erythrocytes.


Synthesis of prostaglandin F 2   by the ovary and uterus.


Radioimmunoassay of prostaglandins.

Radioimmunoassay of prostaglandins.


Development and application of a radioimmunoassay for F prostaglandins.


Termination of second trimester pregnancy with intra-amniotic administration of prostaglandins E 2  and F 2  .


Management of intraoperative proctotomy incidental to total cystectomy for bladder carcinoma.


Pyruvate kinase, hexokinase, and aldolase isozymes in rat liver cells in culture.


Serological cross-reactions between the hemagglutinin subunits of H0N1 and H1N1 influenza viruses detected with "monospecific" antisera. "Monospecific" antisera to the "fragile" hemaglutinnis of H0N1 (PR8) and H1N1 (FM1) influenza viruses detected an asymmetrical cross-reaction between these two strains that could not be explained by a common neuraminidase.


Selective replication of bacteriophage phi29 deoxyribonucleic acid in 6-(p-hydroxyphenylazo)-uracil-treated Bacillus subtilis. Phage phi29 deoxyribonucleic acid (DNA) replicated under conditions where semiconservative DNA production in Bacillus subtilis host cells was blocked with 6-(p-hydroxyphenylazo)-uracil (HPUra). The time of initiation of phi29 DNA replication was not affected by HPUra, and normal quantities of viable phage were produced in the presence of the inhibitor. Studies with conditional lethal mutants of phage phi29 demonstrated the usefulness of HPUra for detection of viral-specific DNA production.


Mixed infections of Bacillus subtilis involving bacteriophage SPO2c 1 . The synthesis of ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) was studied in Bacillus subtilis cells mixedly infected with phages SPO2c(1) and either SP82 or beta22. It was found that cells preinfected 5 min with either beta22 or SP82 could not support significant amounts of RNA or DNA synthesis from the genome of superinfecting SPO2c(1). Conversely, cells preinfected with SPO2c(1) remained susceptible to superinfection even at the midpoint of the latent period. When SP82 was added to cells preinfected 5 min with SPO2c(1), the former greatly inhibited the replication and transcription of the SPO2c(1), DNA, but if superinfection with SP82 was delayed until the 15th min of the SPO2c(1) latent period, RNA and DNA specific for both phages were synthesized. Both viral genomes were transcribed throughout the remainder of the lytic cycle when beta22 was added to cells preinfected either 5 or 15 min with SPO2c(1). In both the SPO2c(1)-SP82 and the SPO2c(1)-beta22 mixed infections, the types of phage-specific RNAs synthesized at a given time in the latent period were similar to those synthesized in single infections with each phage. The association of SPO2c(1) DNA with the host cell membrane in certain of the doubly infected cells was different from that observed in singly infected cells. In the SPO2c(1)-SP82 infection, SPO2c(1) DNA was not released from the membrane; in the SPO2c(1)-beta22 mixed infections, significantly less SPO2c(1) DNA was membrane-bound when beta22 was added before SPO2c(1). However, binding and release of SPO2c(1) DNA was normal in cells infected first with SPO2c(1) and then with beta22. The synthesis of phage-specific nucleic acids and the binding of phage DNA to the membrane are discussed with respect to dominance relationships among these phages.


Penetration of a bacteriophage into Bacillus subtilis: blockage of infection by deoxyribonuclease. Plaquing of a newly isolated phage of Bacillus subtilis, phage 41c, is only 2% efficient in agar containing 200 mug of deoxyribonuclease per ml. Timed deoxyribonuclease addition experiments showed that phage development is blocked in 90% of the cells if deoxyribonuclease is present during adsorption (zero-time samples), whereas 10 min after adsorption the enzyme has little effect (10-min samples). The fate of (32)P-deoxyribonucleic acid label of phage 41c in zero-time samples was compared to that in 10-min samples. In both, about 80% of the label remained with the phage-bacterium complex on initial centrifugation. However, four successive washings removed 90% of the (32)P from the zero-time samples but only 25% from the 10-min samples. In both samples, most of the washed-out label was of low molecular weight. When the time course of interruption of infection by blending was compared with interruption by deoxyribonuclease treatment, the two processes exhibited similar kinetics. It is postulated that both processes block injection at the same site, namely, the point of contact between phage tail and cell wall surface. Partitioning of (32)P label during protoplasting of zero-time and 10-min samples was similar to that observed during washing. For the protoplasting experiments, a quantitative method for plaquing protoplasts was developed. A single bacillus made of several cells can give rise to several protoplast plaque-forming units. Strain 41c was the only phage of seven tested to be inhibited by deoxyribonuclease. No other deoxyribonuclease-sensitive phages have been described.


Measles virus ribonucleic acid and protein synthesis: effects of 6-azauridine and cycloheximide on viral replication. Cycloheximide and 6-azauridine were employed to study the time course of measles virus protein and nucleic acid syntheses in AV3 cells. Synthesis of ribonucleic acid (RNA) essential for infectivity was first detected at 6 hr and increased concurrently with the formation of essential protein. Maximum levels of virus-specific RNA and protein were present by 18 hr, a time when only 5% of progeny virus was detected. Essential RNA and protein syntheses preceded the formation of infectious virus by at least 10 to 12 hr. The time course of RNA and protein syntheses essential for the formation of complement-fixing (CF) antigen and salt-dependent agglutinin (SDA) was also determined. RNA synthesis essential for the formation of SDA was first detected at 2 hr and was present maximally by 6 hr, whereas SDA-protein increased concurrently with the protein essential for infectivity. This suggested that the last protein essential for infectivity may be SDA. RNA synthesis essential for the formation of CF antigen was first detected at 4 hr, while CF-protein increased at 5 hr and preceded SDA-protein and protein essential for infectivity by approximately 3 hr. Reversal of inhibition of protein synthesis by cycloheximide indicated that early protein synthesis (1 to 3 hr) was required for the formation of infectious virus. The data suggest that the relatively long eclipse period observed with measles virus is related to a long maturation period rather than to late formation of early proteins, viral RNA, or structural proteins.


Gene expression during the development of Bacillus subtilis bacteriophage phi 29. I. Analysis of viral-specific transcription by deoxyribonucleic acid-ribonucleic acid competition hybridization. The ribonucleic acid (RNA) specified by bacteriophage phi29 was analyzed to determine its composition at various times in the viral lytic cycle. Although viral-specific RNA was detected immediately after infection, a large increase in the rate was observed at 10 min when DNA synthesis began. phi29 was found to resemble other viruses in that gene expression occurred in two stages which could be defined temporally as "early" and "late." Early RNA appeared before the onset of viral deoxyribonucleic acid (DNA) replication and accounted for approximately 40% of the viral genetic potential. This RNA was also present late in the infectious cycle because of the slow turnover rate of phi29-specific RNA (approximately 10 min half-life) and the continued synthesis of much early viral RNA throughout infection. Late RNA was first detected at approximately the same time as viral DNA replication, although late transcription was not dependent upon DNA synthesis. This RNA was only partially displaced by early RNA in the appropriate competition experiments, suggesting that it contained sequences not present in the early class. Expression of viral genes was sensitive to rifamycin throughout the lytic cycle, the sensitivity resulting from a dependence upon the rifamycin phenotype of the host RNA polymerase.


Gene expression during the development of Bacillus subtilis bacteriophage phi29. II. Resolution of viral-specific ribonucleic acid molecules. The ribonucleic acid (RNA) specified by bacteriophage phi29 was isolated under conditions which minimized physical and enzymatic degradation, reduced aggregation, and enriched for completed molecules. This RNA was fractionated both by sedimentation through sucrose density gradients and electrophoresis through polyacrylamide gels to measure the size and relative amount of each component. Early RNA consisted of six components of molecular weight 0.75 x 10(6), 0.44 x 10(6), 0.37 x 10(6), 0.25 x 10(6), 0.09 x 10(6), and 0.04 x 10(6), accounting for 35% of the coding capacity of phi29 deoxyribonucleic acid (DNA). All of these components except the one at 0.44 x 10(6) were detected when infection occurred in the presence of chloramphenicol. Synthesis of the major early component (0.75 x 10(6)) ceased shortly after the onset of viral DNA synthesis. The other species of early RNA were synthesized throughout the latent period. Three additional components, 1.75 x 10(6), 0.93 x 10(6), and 0.07 x 10(6), appear at late times. The two large RNAs may be polycistronic messenger RNAs corresponding to the seven viral capsid proteins.


Transcription during the development of bacteriophage phi29: definition of "early" and "late" phi29 ribonucleic acid. Bacteriophage phi29 messenger ribonucleic acid (mRNA) production following infection of Bacillus subtilis has been analyzed. Early (e) phi29 RNA, made prior to the onset of phage deoxyribonucleic acid (DNA) replication and exclusively from the light (L) phi29 DNA strand, has been shown by RNA-DNA hybridization-competition experiments to be present throughout the phage latent period. No repression of e RNA production during phi29 development could be demonstrated. Unmodified host RNA polymerase molecules appear to be sufficient for the synthesis of e RNA since phage-specific RNA made in the presence of chloramphenicol hybridizes only to the L strand of phi29 DNA, and this RNA can be effectively competed during hybridization by e RNA. The appearance of late (l) phi29 RNA is coincident with the onset of viral DNA replication. This RNA consists of L DNA strand transcripts which are identical to e RNA and a new class of mRNAs made exclusively from the "heavy" (H) phi29 DNA strand (lH). Protein synthesis in infected cells is required for lH RNA production. Studies with the antibiotic rifamycin demonstrated that synthesis of the major phi29 structural proteins is dependent on production of lH RNA.


Nitrite pharmacodynamics and the hemodynamics of angina.


Cystathionase: immunochemical evidence for absence from human fetal liver.


The influence of synthetic amino acid and casein hydrolysate on the endogenous production and urinary excretion of acid in total intravenous alimentation.


Personality in monkeys: factor analyses of rhesus social behaviour.


Effects of low temperature preservation and route of inoculation on infectivity of Babesia bigemina in blood diluted with glycerol.


Attempts to grow tissue culture cells, infected with Theileria parva, in laboratory animals.


Presumptive diagnosis of Nosema cuniculi in rabbits by immunofluorescence.


Greater curvature gastric diverticulum.

Greater curvature gastric diverticulum.


Bronchoscopical changes after megavoltage therapy in patients with bronchial carcinoma.


Dose measurement in animal experiments. 3. Dose distribution in  60 Co irradiated dogs.


Effect of actinomycin D and mitomycin C on sigma virus multiplication in Drosophila melanogaster.


The metabolism of ( 35 S) homosulphanilamide in the rat, rabbit, guinea pig and monkey.


The complement-requiring neutralization of equine arteritis virus by late antisera.


The conjugation of 4-chloro- and 4-nitro-phenylacetic acids in man, monkey and rat.


Physical procedures for the separation of animal cells.


The hospital as a community facility. Hospital services: needs and costs.


Structure and function of thyroid C cells in newborn rats.


Economics of community health centres.

Economics of community health centres.


Genetic regulation of membrane function in mammalian cells in culture.


The urophysial hydrosmotic factor of fishes. I. Characteristics and similarity to neurohypophysial hormones.


The urophysial hydrosmotic factor of fishes. II. Chromatographic and pharmacologic indications of similarity to arginine vasotocin.


Organ distribution of thyrotropin-releasing hormone.


An early effect of prolactin on the formation of  -lactalbumin by mouse mammary epithelial cells.


Properties of acrosomal hyaluronidase from bull spermatozoa. Evidence for its similarity to testicular hyaluronidase.


Amphibian red blood cell ferritin.

Amphibian red blood cell ferritin.


Chromophore content of blue-green algal phycobiliproteins.


Formation of hybrid proteins form the   and   subunits of phycocyanins of unicellular and filamentous blue-green algae.


Role of enzyme-enzyme interactions in the regulation of glycolysis and gluconeogenesis. Properties of glycogen synthetase isolated from wine kidney.


Threonine synthetase of Bacillus subtilis. The nature of an associated dehydratase activity.


Resolution of enzymes catalyzing energy-linked transhydrogenation. IV. Reconstitution of adenosine triphosphate-driven transhydrogenation in depleted chromatophores of Rhodospirillum rubrum by the transhydrogenase factor and a soluble oligomycin-insensitive Mg ++ -adenosine triphosphatase.


Coupled transport of citrate and magnesium in Bacillus subtilis.


Hemoglobin  3   chains in apes. Primary structures and the presumptive nature of back mutation in a normally silent gene.


Purification and properties of two growth stage-dependent deoxyribonucleic acid polymerases from Lactobacillus acidophilus.


Ribosomal protein neighborhoods. I. S18 and S21 as well as S5 and S8 are neighbors.


Cerebrospinal fluid formation and circulation.


[Neurotoxicity of clioquinol. 1. Oral administration of clioquinol to several species of animals].


Ventricular opacification during carotid angiography secondary to rupture of intracranial aneurysm. Case report.


Methodological development of a system of medical audit.


Comparison between systemic and portal injection of E. coli endotoxin.


The dissociation of gastric acid output and plasma gastrin concentration after calcium infusions in conscious monkeys.


Pulmonary alveolar proteinosis developing from desquamative interstitial pneumonia in long term toxicity studies of iprindole in the rat.


The formal genesis of the transposition of the great arteries.


Specific methods for detection of 5-hydroxytryptamine in carcinoid tumors.


Myocarditis caused by primary oxalosis in a 4-year-old child.


Lobular structure of hemochorial primate placentas, and its relation to maternal vessels.


Cytology of eccrine sweat glands in the opossum.


Immunization of mice against Russian spring-summer virus complex and monkeys against Powassan virus with attenuated Langat E5 virus. Duration of protection.


The attenuation of the 26th mouse brain passage of New Guinea C strain of dengue 2 virus for use in the sequential immunization procedure against group B arboviruses.


Antischistosomal activity of tubercidin in monkeys.


Immunoglobulins in the human eye. Location, type, and amount.


Keratoplasty with glued-on lenses for alkali burns. An experimental study.


Intracranial pressure and ocular hemodynamics.


Accumulation of bicarbonate in intact chloroplasts following a pH gradient.


Hypotension, nitroglycerin, and acute myocardial infarction.


Photooxidative reactions of c-phycocyanin.


Some ultrastructural features in the spermatid of pig tail monkey.


Social, economic, dietary and medical factors in nutrition rehabilitation.


Serum mucoprotein (tyrosine) in protein calorie malnutrition.


High-calorie parenteral alimentation: serum lactic and pyruvic acid levels in immature dogs during infusion with concentrated glucose solutions.


Maximal oxygen consumption, pulmonary function, body composition, and anthropometry of adolescent female athletes.


Chylothorax after Bochadalek herniorrhaphy in a neonate. Treatment with intravenous hyperalimentation.


Effects of thiocyanate and acetylsalicylic acid on the gastric mucosal barrier in rats.


Total parenteral hyperalimentation: infants with chronic intractable diarrhea.


Evaluation of the Lewis test. Lewis test compared to CRP tests.


Effects of fractionated doses of electrons on human skin.


Infection of sheep with Listeria monocytogenes.


Quantitative immunoelectrofocusing of proteins with respect to their heterogeneity.


The location of tryptophanyl groups in human and bovine carbonic anhydrases. Ultraviolet difference spectra and chemical modification.


Quaternary structure of Bacillus subtilis  -amylase. Anomalous behavior in sodium dodecyl sulfate.


Purification and properties of a cross-reacting material related to  -amylase and biochemical comparison with the parent  -amylase.


The occurrence of immunoglobulin fragments, two types of lactoferrin and a lactoferrin-IgG2 complex in bovine colostral and milk whey.


Minor components of bovine  -lactalbumin A and B.


Apparent heterogeneity of bovine  -lactalbumin A and B in DEAE-Sephadex chromatography.


Immunological analysis of particulate antigens of human leukemia.


Permeability increases in cells of Salmonella enteritidis induced by ethylenediaminetetraacetate and temperature treatments.


Quinate metabolism in Pseudomonas aeruginosa.


Characterization of the aminopeptidase from Bacillus subtilis as an extracellular enzyme.


Relationship between Bdellovibrio bacteriovorus 6-5-S and autoclaved host bacteria.


Thyroid secretion rate and thyrocalcitonin content of the thyroid in female rats.


Preferential incorporation of tunicamycin, an antiviral antibiotic containing glucosamine, into the cell membranes.


Cytochemical evidence for RNA synthesis inhibition by fumagillin.


Effect of alcohol on serum folate level. Alcohol was given orally and intravenously to normal and chronic alcoholic volunteers to study its effect on folate metabolism. Oral alcohol, given to nine subjects on a low folate diet, caused a greater fall in serum Lactobacillus casei folate levels than that seen in eight subjects on a low folate diet alone. This alcohol-induced fall in serum folate level occurred largely during the 1st day of the protocol. Although brief infusions of intravenous ethanol had no effect on serum folate level, a 13 h infusion caused a striking fall in serum folate level between the 8th and 10th h. When ethanol was stopped, the serum folate level returned rapidly to normal. Two chronic alcoholic subjects with different basal levels of serum folate were studied for several weeks on a low folate diet plus alcohol. The serum folate level fell promptly in each subject, rose when alcohol was temporarily stopped, and fell when alcohol was resumed. Folate-deficient megaloblastic anemia developed in 3 wk in the subject with initially marginal serum folate levels, but failed to develop in almost 7 wk in the subject with normal folate stores, as reflected by initially high serum folate levels. Thus, the alcohol-induced fall in serum folate level was apparently not a result of depletion of folate stores. In vitro experiments ruled out an assay artifact as an explanation for the alcohol-induced fall in serum L. casei folate level. It seems likely that alcohol interferes with the delivery of n-5-methyltetrahydrofolic acid from storage areas.


Properdin anc C3 proactivator: alternate pathway components in human glomerulonephritis. Serological and immunopathological studies of human glomerulonephritis have suggested that alternate pathways of activation of the third component of complement may be important in some forms of glomerulonephritis. We have investigated the role of two alternate pathway proteins, properdin and C3 proactivator, in 22 patients with chronic membranoproliferative glomerulonephritis, 21 patients with systemic lupus erythematosus, 20 patients with acute poststreptococcal glomerulonephritis, and 19 patients with other forms of renal disease. C3 (measured at beta(1)A), properdin, and C3 proactivator were assayed by single radial immunodiffusion. In sera with low beta(1)A (< 2 SD), mean properdin was most significantly decreased in patients with acute poststreptococcal glomerulonephritis but was also significantly decreased in chronic membranoproliferative glomerulonephritis and in untreated systemic lupus erythematosus. Properdin levels in other renal disease, acute glomerulonephritis, and chronic membranoproliferative glomerulonephritis with normal beta(1)A levels were not significantly different from normal. A positive correlation between beta(1)A and properdin levels in individual sera was present in all diseases except systemic lupus erythematosus. Serum C3 proactivator was markedly decreased in active systemic lupus erythematosus and there was a positive correlation between beta(1)A and C3 proactivator levels in systemic lupus erythematosus and other renal diseases but not acute poststreptococcal glomerulonephritis. Properdin in fresh sera from four patients with systemic lupus erythematosus and five with chronic membranoproliferative glomerulonephritis showed increased migration toward the cathode on immunoelectrophoresis, suggesting in vivo change of the properdin molecule. The observation of reduced serum levels of properdin and C3 proactivator and altered electrophoretic migration of properdin in some patients with glomerulonephritis provide new evidence for participation of these alternate pathway proteins in glomerulonephritis.


Permanent cardiac pacing experience with arrhythmias caused by Chagas' disease and other cardiopathies.


Regular-insulin doses in monkeys measured by an operant schedule of titrated sucrose reinforcement.


Secondary hypercalcemic hyperparathyroidism.


Genetic aspects of serum IgE levels in atopic families.


Census of Aspergillus colonies in the air comparison between London, Paris, Lyon, Marseilles.


Disodium chromoglycate in the treatment of perennial allergic rhinitis.


Leukocyte phagocytosis and serum bactericidal activity in chronic renal failure.


The use of Dixon plots to study enzyme inhibition.


Hybridisation of aldolase from Drosophila, blocked at the active site, with native C aldolase from calf brain.


Purification and characterization of a tyrosinase from Streptomyces glaucescens.


On the peptidoglycan of the cell walls of Pseudomonas aeruginosa.


Inactivation of antithrombin 3 by fatty acids.


Nutrition in a New Guinea highland community.


"Quac--stick"--field measure for quick assessment of nutritional status of preschool children.


Chromatographic fractionation of the lipids of Tetrahymena pyriformis.


Nitrosation of tertiary amines and some biologic implications.


Severe angina pectoris. Considerations of surgical and medical management.


Infections in heroin addicts.

Infections in heroin addicts.


Metabolic effects following a short and medium chain triglycerides load in dogs. 3. Infusion of trinonanoate.


Patterns of sexual behavior and anatomy of copulatory organs in macaques.


Behavioral and brain correlations in early life nutritional deprivation.


Improved control of epilepsy by monitoring plasma ethosuximide.


Allosteric inhibition of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase by tyrosine, tryptophan and phenylpyruvate in Pseudomonas aeruginosa.


Purification and properties of glycogen synthase I from bovine heart: two kinetic forms.


Purification and properties of glycogen synthase I from skeletal muscle: two kinetic forms.


Specificity of activation of glycogen synthase I from skeletal muscle and heart.


Galactosyltransfer in mouse mastocytoma: purification and properties of N-acetyllactosamine synthetase.


Defects and disabilities of thalidomide children. The range of defects and disabilities in thalidomide children is very much wider than is generally realized. The defects of 154 children are described and classified. Their disabilities range from incapacitating to negligible.


Rh immunization in ruptured tubal pregnancy.


Anticholinergic blocking of prostaglandin-induced bronchoconstriction.


Studies on the prophylactic and radical curative activity of RC-12 against Plasmodium cynomolgi in Macaca mulatta. The compound 4-(2-bromo-4,5-dimethoxyphenyl)-1,1,7,7-tetraethyldiethylenetriamine (RC-12) has been shown to be active against the exoerythrocytic (EE) stages of the malaria parasite. Experiments on Plasmodium cynomolgi in rhesus monkeys showed that single weekly doses of 25 mg per kg of body weight would prevent the development and/or maturation of EE stages. The usefulness of RC-12 for effecting radical cures is, however, still open to doubt.


Chromosomal mapping of Brucella abortus, strain 19.


Protease production by thermophilic fungi.


Abundant growth and sporulation of Bacillus sphaericus NCA Hoop 1-A-2 in a chemically defined medium.


A replicative form of the DNA of minute virus of mice.


Cultivation techniques for the erythrocytic stages of malaria parasites. The study of the biochemistry, physiology, and immunology of plasmodia has been restricted by the difficulty of maintaining the parasites in isolation from the host. Some success has been achieved in cultivating them in vitro, using tissue cultures and chick embryo techniques to study exoerythrocytic states and the sporogonic cycle, but no completely successful method has been found for studying the asexual and sexual stages of plasmodia in circulating red blood cells. The relative slowness with which techniques for continuous in vitro cultivation have been developed is the result of inadequate knowledge of the biochemistry of the parasites and of the blood and its constituents. However, radioactive labelling techniques applied to P. knowlesi cultures are beginning to yield data of fundamental importance. Existing methods for the short-term in vitro cultivation of plasmodia are potentially very useful for analysing malarial antigens, for developing vaccines, and for screening and studying antimalarial drugs. Investigations of the physicochemical requirements for the in vitro preservation of red blood cells are required, and more emphasis should be given to the study of plasmodia with longer cycles. Differences between the metabolism of plasmodia in vivo and in vitro should be studied and the growth factors in normal plasma identified. Studies of the membrane of the parasites and of the red blood cells, of the immune response, and of extracellular methods for the cultivation of plasmodia should be extended.


Physiological and biochemical properties of the exudate associated with developing sclerotia of Sclerotinia sclerotiorum (Lib.) DeBary.


On the mycophage of Penicillium chrysogenum.


Rhesus immunization in Rhodesia.

Rhesus immunization in Rhodesia.


Phyletic and idiosyncratic determinants of gender identity.


Studies of membrane formation in Tetrahymena pyriformis. VI. Variations in the rate of lipid synthesis during division of heat-synchronized cells.


Evidence for existence of messenger ribonucleoprotein complexes in Tetrahymena pyriformis.


Synthetic activity of isolated rat liver nuclei. I. Ultrastructural study at various steps of isolation.


Old age and the quality of life.

Old age and the quality of life.


Effects of disruptive selection. X. Selective migration.


Thermal regulation of the immune response in South American toads (Bufo marinus).


Immune response in Xenopus laevis and immunochemical properties of the serum antibodies.


Studies of porcine secretory IgA and its component chains in relation to intestinal absorption of colostral immunoglobulins by the neonatal pig.


Isoelectric focusing of long-acting thyroid stimulator immunoglobulin G.


Urinary immunoglobulins in the adult pig.


Effect of thyrotropin-releasing hormone on human pituitary thyrotropin, prolactin, placental lactogen, and chorionic thyrotropin.


A homologous radioimmunoassay for human prolactin.


Thyrotropin releasing hormone (TRH) mediated thyroid-stimulating hormone (TSH) release from human anterior pituitary tissue in vitro.


The effects of specific metals on the antigenic structure of alkaline phosphatase from Escherichia coli.


Experimental allergic encephalomyelitis in inbred and outbred mice.


Chicken antibodies to group A streptococcal carbohydrate.


Potentiated reagin response: initiation with minute doses of antigen and alum followed by infection with Nippostrongylus brasiliensis.


Studies on the secretory immunologic system of fowl. 3. Serum and secretory IgA of the chicken.


Adjuvanticity of mycobacterial RNA and poly A:U for induction of experimental allergic encephalomyelitis in guinea pigs.


Studies on the mechanism of lymphocyte-mediated cytolysis. I. The role of divalent cations in cytolysis by T lymphocytes.


Studies on the mechanism of lymphocyte-mediated cytolysis. II. The use of various target cell markers to study cytolytic events.


A human IgG myeloma protein with anti- 2 macroglobulin antibody activity.


Clinical evaluation of a new indirect indicator of serum-free thyroxine concentration.


Clinical manganism and exposure to manganese in the production and processing of ferromanganese alloy.


Electrical stimulation of the carotid sinus nerves.


Isolation and characterization of multiple forms of phenylethanolamine N-methyltransferase.


Effect of shadow trays on surface dose and build-up for megavoltage radiation.


A method for the calculation of dose in the radiation treatment of Hodgkin's disease.


Ribonucleic acid synthesis in porcine cell cultures infected with transmissible gastroenteritis virus.


Brucella melitensis Rev. 1 and Brucella abortus 45-20 vaccines in goats: serologic tests.


Brucella melitensis Rev. 1 and Brucella abortus 45-20 vaccines in goats: pattern of immunoglobulin production after vaccination and challenge.


Membrane filter technique for enumeration of Pseudomonas aeruginosa. A membrane filter procedure for the quantitation of Pseudomonas aeruginosa (mPA procedure) has been developed. Through the use of inhibitors and an elevated incubation temperature, the level of background microbial flora was decreased approximately 10,000-fold. Using P. aeruginosa cells suspended in sea water and held for 24 hr, between 70 and 100% of the "viable" cells could be recovered by the mPA procedure. Assay variability was found to be insignificant. The recoveries of P. aeruginosa from surface (fresh and salt) waters, potable waters, and sewage by the mPA procedure exceeded those obtainable by current methods. Subsequent to its development and evaluation, the mPA procedure was used at three other laboratories for the enumeration of P. aeruginosa in potable and recreational waters and in sewage samples. It was found amenable to routine use, and confirmation of typical colonies approached 100%.


Comparative studies on the detoxification of aflatoxins by sodium hypochlorite and commercial bleaches. Cultures of Aspergillus flavus and aflatoxins were destroyed by a commercial bleach (Clorox; active ingredient, NaOCl) or analytical reagent grade NaOCl at 7.0 x 10(-3) M NaOCl in 5 days. Addition of Clorox or NaOCl at 2.8 x 10(-3) M to the fungal growth medium prior to inoculation completely inhibited the fungal growth. Aflatoxin production was inversely proportional to the logarithm of NaOCl concentration and time of treatment. Clorox and NaOCl were equally effective on aflatoxins, but fungal cells were lysed more readily by Clorox than by NaOCl. Mycelia older than 8 days lysed more readily than younger ones. Most conidia survived concentrations below 1.4 x 10(-3) M. The lowest effective concentration for a 2-hr treatment was 8.8 x 10(-3) M which is well below the Clorox concentration recommended for routine laboratory decontamination of aflatoxins. Mice and rats injected with aflatoxins and aflatoxins incompletely destroyed by Clorox died within 72 hr and had typical liver and kidney damage caused by aflatoxins. However, animals injected with NaOCl or Clorox or Clorox-destroyed aflatoxin extracts survived and showed no obvious liver or kidney damage.


Effect of pH and oxygen tension on staphylococcal growth and enterotoxin formation in fermented sausage. Commercial fermented 0sausages that contained significant numbers of viable coagulase-positive staphylococci were found to have the growth localized in the outermost areas of the sausage where oxygen tension was highest. Staphylococci were found to be more acid-tolerant aerobically than anaerobically. With chemical acidulation of sausage, growth could be controlled both aerobically and anaerobically with approximately 1.5% glucono delta lactone. Biological acidulation with a high inoculum of Pediococcus cerevisiae inhibited anaerobic staphylococcal growth but failed to suppress aerobic growth completely. A staphylococcal count of approximately 4 x 10(7) cells/g of sausage appeared to be necessary to produce detectable enterotoxin A within 24 hr in sausage. A minor difference existed in the relative rates of production of the different types of enterotoxin. Detectable enterotoxin A was produced in 24 hr in sausage held in atmospheres containing 10, 15, and 20% oxygen. In an atmosphere containing 5% oxygen, toxin was detected after 48 hr of incubation. No toxin was detected after 120 hr under anaerobic conditions. Most staphylococcal strains tested initiated growth and produced detectable enterotoxin aerobically at a pH of 5.1 in broth media. Anaerobically, however, most strains failed to produce detectable enterotoxin below pH 5.7.


Convenient assay for staphylococcal nuclease by the metachromatic well-agar-diffusion technique. The metachromatic agar-diffusion (MAD) microslide technique was adapted for quantitative assay for staphylococcal thermonuclease in heterogeneous systems, such as milk and broth. When an enzyme-containing solution was placed in a well cut in the agar, a bright pink halo was obtained. The diameter of the pink zone of hydrolysis was related to time and temperature of incubation and to nuclease concentration. Concentrations of nuclease as low as 0.005 mug/ml and as high as 2.0 mug/ml were conveniently determined after 3 hr at 37 C.


Agglutination of African primate and rodent erythrocytes by adenoviruses, reoviruses, and enteroviruses. African nonhuman primate and rodent erythrocytes were tested for agglutination by adenoviruses, reoviruses, and enteroviruses. Squirrel erythrocytes were agglutinated by reovirus serotypes and adenovirus types 3, 11, 16, and 21. Adenoviruses also agglutinated brazza monkey erythrocytes to the same titers as those obtained with either rhesus or grey monkey cells. Prototype reovirus types 1 and 2 agglutinated grey monkey erythrocytes to much lower titers than either squirrel or human group O red cells. Among the enteroviruses tested, only echovirus types 7 and 12 agglutinated grey, red-tail, brazza, and rhesus monkey erythrocytes. The specificity of agglutination of squirrel, grey, and brazza monkey erythrocytes by reoviruses, echoviruses, and adenoviruses, respectively, was confirmed by hemagglutination-inhibition tests. The titers obtained were similar to those obtained with erythrocytes usually used in these tests. Erythrocytes of bush babies, potto unstriped grass mice, swamp rat, rusty-nosed rat, bush rat, harsh-furred mice, soft-furred rat, and giant rat were not agglutinated by adenoviruses, reoviruses, or enteroviruses.


Formation and fate of the otoconia. Scanning and transmission electron microscopy.


Microtitration of Bacillus cereus hemolysin. A microtiter procedure for the quantitation of Bacillus cereus hemolysin is described.


Dicarboxylate omega-amidase of Bacillus subtilis-168: evidence for a membrane-associated form.


Comparative characteristics of human and porcine staphylococci and their differentiation in burn xenografting procedures. Staphylococcus epidermidis from porcine skin differed from human cutaneous S. epidermidis in that the former strains were principally of the Baird-Parker biotype III group. The porcine-type strains were more proteolytic on casein and gelatin than were human strains, which were primarily of biotype II. Porcine strains were also elastolytic. Using supernatant fluids of broth cultures, the biotype II strains, but not the type III strains, were lipolytic in action on triolein. Both types of staphylococci were similar in enzymatic activities on Tween 80, egg yolk, and tributyrin. Elastase activity was not found in broth supernatant fluid of these bacteria. The porcine strains were retarded or inhibited from growing in media at pH 5.5. Action on casein agar followed by demonstration of elastase activity were used as markers to detect the porcine S. epidermidis strains in xenografts and on human burn wound grafting sites.


Purification and properties of dicarboxylate omega-amidase from Bacillus subtilis 168 and Thermus aquaticus YT-1.


The nail in Darier-White disease.

The nail in Darier-White disease.


Juvenile xanthogranuloma and neurofibromatosis.


Experimental protein-energy deficiency in rats. Ratio of serine+glycine to threonine as an index of deficiency.


Severe hyponatraemia in hyperlipaemic diabetic ketosis.


Rate of passage of digesta in sheep. 1. The effect of level of food intake on marker retention times along the small intestine and on apparent water absorption in the small and large intestines.


The collection and analysis of human fallopian tubal fluid.


Reversible male sterilization: current status and future directions.


Monkey anti-placental serum as an abortifacient. The abortifacient effect of antiplacental sera was confirmed with the use of goat and rabbit anti-Rhesus monkey placental globulin (IgG).  To induce abortion, a total of 600-1000 mg IgG (from goats and rabbits treated to produce antiplacental sera) was injected intravenously over 3 days into 9 monkeys at 39-54 days of gestation.  As a control a similar dose of normal goat globulin was injected into 3 pregnant monkeys.  Abortion occurred in 7 of the monkeys injected with IgG; 2 of these abortions were after 2 injection sequences, and 1 of these 2 may have been the result of something other than an immunoreaction.  Abortion occurred in none of the monkeys injected with normal goat globulin.  No anaphylaxis or harmful reactions were observed in IgG-injected monkeys.  In another portion of the study unabsorbed antiplacental globulin reacted with monkey serum, liver extract, and kidney extract, as well as with monkey placental extract.  IgG absorbed with serum or organ extract reacted only with placental extract.  No reaction was found between the absorbed antiplacental globulin and monkey placental lactogen or chorionic gonadotrophin.  Cytotoxicity titers appeared to parallel the effectiveness of the IgG preparation in producing abortion.  With the use of an immunofluorescent technique it appeared that the injected antibody(s) reacted with placental specific antigen localized on the trophoblast.  It is thought that the reacted tissue is then destroyed with participation of complement.  The abortions were apparently caused by passive immunization.


Inhibition of implantation with antibodies against uterine fluid and blastocysts in the rat.


The ecology and behavior of squirrel monkeys (Saimiri oerstedi) in a natural forest in western Panama.


Dominance hierarchy in squirrel monkeys (Saimiri sciureus). Role of the gonads and androgen on genital display and feeding order.


Sociometrics of Macaca mulatta. I. Linkages and cliques in grooming matrices.


Communication among captive talapoin monkeys (Miopithecus talapoin).


Spatial factors and the behavior of nonhuman primates.


The order of movement of adult male and black infant baboons (Papio anubis) entering and leaving a potentially dangerous clearing.


Education and the hospital: President's Committee on Health Education.