Function of the reticuloendothelial system in whole-body irradiated mice.


Studies on messenger RNA from Euglena gracilis. I. Techniques of saline fractionation and characterization of messenger RNAs from dividing cells.


Ribosomal precursor particles from plant nucleoli.


Direct fluorometric determination of urea in urine.


Tyrosinase activity in the Wolffian lens regenerating system.


Partial characterization of several protein and amino acid metabolizing enzymes in the cellular slime mold Dictyostelium discoideum.


Neurological disorders and growth disturbances in infancy and childhood.


Localization of carbonic anhydrase activity in the vertebrate nephron.


Transovarial transmission of Rickettsia tsutsugamushi in Leptotrombidium (Leptotrombidium) arenicola Traub (Acarina: Trombiculidae).


Comparative analysis of amniotic fluid bilirubin.


Purification and properties of a tyrosinase from Streptomyces glaucescens.


Gestational and postpartum plasma diamine oxidase values.


Studies on the rising phase of the action potential in mammalian cardiac ventricular fibers.


Growth of simian adenovirus SA7 during arginine starvation.


Self-parasitism in Aspergillus flavus.

Self-parasitism in Aspergillus flavus.


Delayed hypersensitivity in the guinea pig immunized with killed tubercle bacilli in adjuvant. 3. Peritoneal cell migration inhibition and macrophage cytophilic antibodies to tuberculin purified protein derivative.


Comparison of numerical procedures for grouping pseudomonas bacteriophages according to lytic spectra.


A transformed Yule correlation coefficient employed in numerical grouping procedures on bacteriophage lytic spectra.


Changes in amniotic fluid volume and total solute concentration in the rhesus monkey following replacement with distilled water.


A mass screening program for the detection of gonorrhea. A 2 1/2 year gonorrhea screening program in Chicago showed 7.5% of women tested to be positive for Neisseria gonorrhoeae organisms with a single cervical culture.  Young patients, unmarried patients, and black patients had the highest attack rates.  Over 98% of those with positive cultures were treated, and this program has probably contributed to a decline in reported gonorrhea morbidity in the first 6 months of 1971.  In another group 4816 women seen at a venereal disease clinic, 24.4% were positive at the cerivx, rectum, or both.  The addition of rectal tests to cervical tests is productive of many cases that would otherwise be missed.  3 million units of procaine penicillin G with 2% aluminum monostearate intramuscularly is an inadequate treatment for women with gonorrhea.


Immunoglobulin D in pregnancy.

Immunoglobulin D in pregnancy.


Automatic red cell washing machine for quantitative assay of anti-D concentration.


Catfish antibodies to blood group substances. II. Cross-reacting anti-A and B antibodies.


Difference spectra and extinction coefficients of P 700 .


Photooxidation of 3,3'-diaminobenzidine by blue-green algae and Chlamydomonas reinhardii.


Components of sodium and chloride flux across toad bladder. The effect of transepithelial potential difference (psi) on Na and Cl flux across toad bladder was assessed by measuring isotopic flux between identical media at various values of psi. The contribution of edge damage to ionic permeability was eliminated, resulting in relatively high spontaneous psi (-97 +/-4 mv) and low electrical conductance g. Bidirectional Na fluxes were measured simultaneously. Unidirectional Cl fluxes were measured in paired hemibladders at psi = 0 mv or -97 mv. Net Na flux J(Na), at psi = 0 mv, was slightly less than short-circuit current (SCC). At psi = -97 mv, J(Na) averaged 17% of SCC, and was sometimes zero. DeltaJ(Na)/Deltapsi (= g(+)) averaged 60% of g between -97 mv and +75 mv; at -150 mv, g(+) fell, indicating rectification. Analysis of unidirectional Na fluxes indicates low passive conductance (1.5 mumho/mg wet weight), a bidirectional, electrically neutral flux of approximately 0.13 mua/mg, and relatively large conductance of the active transport path at psi >/= -97 mv. The absence of appreciable transstimulation of serosal (S)-to-mucosal (M) Na flux (in response to increasing mucosal Na concentration) indicates that the electrically neutral flux is not exchange diffusion in the usual sense. Analysis of Cl fluxes indicates similar values for passive conductance and neutral flux, suggesting linked neutral flux of Na and Cl. Either the electromotive force of the Na pump E, its conductance g(a), or both are strong functions of psi. The product of these two quantities, Eg(a), is a measure of the "transport capacity" at any given value of psi, independent of the direct effect of psi on J(Na) through the pump path. Eg(a) varies with psi. Hence estimation of the net Na flux or current at any one value of psi, including psi = 0, fails to reveal the maximal transport capacity of the pump, its resting electromotive force (when J(Na) = 0 through the pump), or the dependence of transport capacity on potential.


Review of some recent studies in the National Institute of Nutrition Hyderabad.


Schistosomicides. 2.  Derivatives of 2,3,4,4a,5,6-hexahydro-1H-pyrazino(1,2-a) quinoline.


Evaluation of non-phenolics by a quantitative technique.


Novel anthelmintic agents. 6. Pyrantel analogs with activity against whipworm.


Structure of hydroxycotinine, a nicotine metabolite.


Chronic cerebrovascular insufficiency in the elderly. Perspectives on treatment.


Hypersensitivity pneumonitis.

Hypersensitivity pneumonitis.


Uncommon presentations of pulmonary tuberculosis in adults.


The purification of amyloid fibril proteins.


Purification of Chlorella malate dehydrogenase.


Isolation of   2  macroglobulin subunits from human serum.


Does the striate cortex begin reconstruction of the visual world?


Beta-trace protein concentration in CSF in neurological disorders.


Aggressive surgical treatment for caustic injury of the esophagus and stomach.


A bronchogenic cyst successfully treated by mediastinoscopy.


Guinea pig heterophil and eosinophil peroxidase.


Fate of L-(3,5- 3H) tyrosine in cell-free extracts and tissue cultures of melanoma cells: a new assay method for tyrosinase in living cells.


The isolation and characterization of IgD from dysgammaglobulinemic serum.


Kinetics of the in vitro development of cytotoxicity against alloantigens.


Separation and characterization of birch pollen antigens with special reference to the allergenic components.


Alteration of immunological reactivity in chickens by the in ovo administration of antisera against central lymphoid tissue.


Effect of therapeutic dosages of prednisone on human immunoglobulin G metabolism.


A comparison of immediate-type respiratory reactions to immunologic and pharmacologic agents in rhesus monkeys.


Mechanisms of active transport in isolated membrane vesicles. IV. Galactose transport by isolated membrane vesicles from Escherichia coli.


The synthesis of biologically active pteroyloligo- 0L-glutamates (folic acid conjugates). Evaluation of ( 3 H)pteroylheptaglutamate for metabolic studies.


Amino acid control of messenger ribonucleic acid synthesis in Bacillus subtilis.


Purification and some physicochemical properties of staphylococcal enterotoxin E.


Vitamin A transport in rat plasma. Isolation and characterization or retinol-binding protein.


Evidence of phenylalanine transaminase activity in the isoenzymes of aspartate transaminase.


Signal frequency and shock probability as determinants of prolonged vigilance performance in rhesus monkeys. The effects of changing signal frequency on a prolonged vigilance task were investigated by systematically increasing the average inter-trial interval between successive signals. During a 6-hr watch, vigilance performance remained constant when the rate of signal presentation was 40, 20, and 10 per hour. When the rate of signal presentation was reduced to 7 or 4 per hour, marked decrements in detection performance were observed. Similar vigilance decrements occurred when the average rate of signals per hour was kept constant (10 per hour) and the probability of receiving a shock for missing a signal was systematically varied. The results of this study indicate the importance of reinforcement factors in the control and maintenance of vigilance performance.


Insights into public assistance medical care expenditures.


Gonococcal meningitis. A review.

Gonococcal meningitis. A review.


Reuse of dialysis coils.

Reuse of dialysis coils.


Transmission of scrapie to the cynomolgus monkey (Macaca fascicularis).


Suppression of the immune response: reversal of the disease state with antigen in allergic encephalomyelitis.


Australia antigen as a marker of propagation of the serum hepatitis virus in liver cultures.


Absence of eclipse phase in scrapie mice.


Effect of prostaglandin E 2  and aspirin on the secondary aggregation of human platelets.


Australia antigen in Singapore. II. Differential frequency in Chinese, Malays and Indians.


24-hour survival of ACD- and CPD-stored red cells. 1. Evaluation of nonwashed and washed stored red cells.


Surface films of protein and a protein-lipopolysaccharide complex extracted from Pseudomonas aeruginosa.


Severe visual impairment in Swedish children.


Possibility of the use of some physical-chemical agents for the differentiation of phages Pseudomonas aeruginosa.


Clinical-angiographic investigations in hepatic cirrhosis.


Some diagnostic potentialities of the aerosol scintigraphy of the lungs.


Finger print patterns of Turks from the region of Kardzali.


Prolactin and growth hormone levels in serum from cavernous sinus of rhesus monkeys.


Thyrotropin response to thyrotropin releasing hormone in normal females over forty.


Salicylate-induced increases in free triiodothyronine in human serum. Evidence of inhibition of triiodothyronine binding to thyroxine-binding globulin and thyroxine-binding prealbumin. Addition of sodium salicylate to human serum at concentrations often obtained during aspirin therapy causes 100-200% increases in free triiodothyronine (T(3)) and free thyroxine (T(4)) as estimated by ultrafiltration. The increase in free T(3) was unexpected since previous data had suggested that salicylate inhibits binding of T(4) only to thyroxine-binding prealbumin (TBPA) and that T(3) is not bound to this protein. Using ultrafiltration techniques, we demonstrated binding of T(3) to TBPA. The affinity constant for T(3)-TBPA binding appears to be slightly greater than that for albumin-T(3) binding. While salicylate inhibits the binding of T(3) (and T(4)) to TBPA, it can be predicted that little change will be observed in the free T(3) (or free T(4)) without inhibition of thyroid hormone binding to thyroxine-binding globulin (TBG). Using a competitive-binding protein displacement technique, it has been shown that sodium salicylate, like diphenylhydantoin (DPH), inhibits the binding of T(3) and T(4) to TBG. The magnitude of the increase in free T(3) and free T(4) induced by salicylates suggests that interference with TBG binding is its major effect. Aspirin was administered orally to two normal subjects in quantities sufficient to obtain serum salicylate levels of 20-25 mg/100 ml. This resulted in a decrease of 20-30% in total serum T(3) and T(4) levels. This decrease in T(4) levels is similar in magnitude to that previously observed in subjects receiving DPH. Unlike what has been observed with DPH treatment, therapeutic salicylate levels are associated with increases of 50-75% in the unbound fraction of both T(3) and T(4) which persist throughout an 8-10 day treatment period.


C1r deficiency: an inborn error associated with cutaneous and renal disease. The studies of sera from two siblings with C1r deficiency are described. The brother (18 yr old) has shown clinical manifestations resembling lupus erythematosus for 5 yr, and the sister (24 yr old) has had arthralgia and recurrent episodes of rhinobronchitis since early childhood. Three siblings have died: one brother died at age 12 with symptoms similar to the disease of the male patient studied here, and two other siblings died in infancy, probably from infection. The low hemolytic C1 activity of the patients could be restored by the addition of purified C1r to their sera. Bactericidal activity and immune adherence were found to be impaired. When alternate pathways of the complement system were studied, both sera permitted activation of terminal components with endotoxin and cobra venom factor. These findings support the view that an alternate pathway for activation of the terminal portion of the complement cascade exists which does not utilize the conventional pathway operating through the usual early components.


Syphilis serology with lipoidal antigen: the meaning of positive reactions.


Serum lipid-lipoprotein profiles in rabbits infected with Mycobacterium bovis, Listeria monocytogenes, Pasteurella pseudotuberculosis and with M. avium.


Chlorinated hydrocarbon residues in airborne fallout.


Conservatism in the evolution of immunoglobulin.


Genetic aspects of viral diseases of animals.


Ionizing radiation and vincristine: possible neurotoxic synergism.


Neuromuscular disorders of the esophagus.


Etiology, diagnosis and management of pericardial effusion after irradiation.


Compatibility and stability of disodium carbenicillin in combination with other drugs and large volume parenteral solutions.


Intermediate benefit analysis--Spencer's dilemma and school health services.


The social and economic impact of hemophilia--a survey of 70 cases in Vermont and New Hampshire.


Disease in children due to mycobacteria other than Mycobacterium tuberculosis.


A method of characterizing tuberculins.

A method of characterizing tuberculins.


Another attempt to induce high altitude pulmonary edema in monkeys.


Electron microscopy of the Macaca mulatta brain after repeated applications of electric current.


Cutaneous lesions associated with benign gonococcaemia.


Determinants of flow through internal mammary artery implants.


Correlation between neuropathogenicity of Coxsackie A 7 virus and its in vitro markers.


The nature of the reflux material in reflux oesophagitis.


Markers for measles virus. II. Tissue culture properties.


Transplantation of the endocrine pancreas in guinea pigs.


A case of carcinoma of the colon in association with neurofibromatosis.


A case of intraluminal diverticulum of the duodenum.


Influence of nitroglycerin on myocardial metabolism and hemodynamics during angina induced by atrial pacing.


Automated fluorometric procedure for measurement of creatine phosphokinase activity.


Alkaline phosphatase subunits in the culture filtrate of Pseudomonas aeruginosa.


Sensitive, rapid quantitation of serum and urinary protein by electroimmunodiffusion.


Single radial immunodiffusion of undiluted serum proteins.


Blood lead determinations by atomic absorption spectrophotometry, with use of the Delves sampling-cup technique: effect of various anticoagulants.


Financing fertility-related health services in the United States, 1972-1973: a preliminary projection. 2 debates over national policy are currently underway: 1) a major review of the national policies has been demanded by virtually all segments of the American people; and 2) an evaluation has been undertaken of policies which influence the  growth of U.S. population, of the kinds of changes in population growth, and distribution which would be socially and economically desirable, and of the means available and acceptable to bring about the desired changes.  Crucial to these policy discussions are the nature and scope of health services.  This article d escribes preliminary research to estimate the costs to U.S. society of f ertility-related health services.  Fertility control through voluntary sterilization, contraception, pregnancy termination, and medical treatment of infertility make up 1 group of services; maternity care forms a 2nd group; and pediatric care is a 3rd essential service.  It is clear from this study that many fertility-related health services are not covered by private health plans and that additional expenditure is needed to upgrade hospital services and improve health care.  The study also raises questions about deficits in service to people who are not re ached by the medical care system at all and those who receive care of low quality.  Women, especially those in the low-income bracket, need more aid.  The range of costs shown here suggests that comprehensive financing of fertility-related health services is well within the nation's fiscal capabilities.


National health insurance: lessons from "down under".


Intestinal immunoglobulins in shigellosis.


Phi29 bacteriophage structural proteins.


Tragic misadventure with hexachlorophene.


Survival of pathogenic microorganisms on human skin.


Antiserum against tyrosinase from mouse melanoma.


Active secretion of thyroxine into bile: the role of tissue thyroxine-binding sites. 1. An investigation has been made into factors regulating the secretion of thyroid hormones into bile. The preparation employed was the isolated rat liver perfused with a modified Tyrode solution.2. Tracer amounts of radioactive thyroxine were injected into the Tyrode solution just before its entry into the liver. This allowed the thyroxine-binding sites in the tissue to capture most of the injected hormone. It was found that this tissue-bound hormone was then gradually secreted into the bile and that the process could be resolved into two components. There was a steady, continuous secretion of hormone, which maintained a fairly constant bile:liver ratio in thyroxine concentration of about 2.3. There was also, superimposed upon this, an extra transient secretion of thyroxine occurring just after the injection. This transient effect was abolished by injecting the hormone at 22 degrees C and it was probably due to the sudden flood of free thyroxine into the tissue during the 15 sec injection period.3. Very similar results were obtained with tri-iodothyronine except that the secretion process maintained a higher bile:liver ratio of about 3.7.4. The secretion mechanism was extremely sensitive to changes in temperature and it was able to generate very high concentrations of free thyroxine in the bile. It was concluded that the over-all process was probably an active one.5. The presence of 30% bovine serum in the perfusion fluid did not reduce the bile:liver thyroxine ratio even though it caused a massive fall in the amount of thyroxine in the tissue.6. These results show that the rate of thyroxine secretion into bile is determined by the total amount of thyroxine trapped in the tissue. It is not directly related to the concentration of free thyroxine in the perfusing medium.7. It is suggested that the secretion mechanism works from a small intracellular pool of free thyroxine which is in rapid exchange with the large pool of tissue-bound hormone, and that thyroxine exchange between this small intracellular pool and the free thyroxine pool in the plasma is relatively slow.


On the physiological response of the cerebral cortex to acute stress (reversible asphyxia). 1. Rhesus monkey (Macaca mulatta) foetuses were delivered by Caesarean section 3-10 days before term. Aortic blood and cerebrospinal fluid (c.s.f.) samples were taken, the latter from the cortical subarachnoid space and the cisterna magna. The umbilical cord was clamped and foetal breathing prevented for 14-17 min. Blood and c.s.f. were sampled further during this total asphyxiation and for up to 24 hr thereafter.2. The [K(+)] in the cortical subarachnoid fluid started to rise within 2-3 min after the onset of asphyxia and increased up to 7 times the normal level. The [K(+)] of blood plasma and cisternal fluid also increased, but much more moderately. All these effects reversed rapidly upon resuscitation of the foetus.3. A pronounced rise in the cortical subarachnoid fluid [glucose] and a lesser effect on cisternal fluid [glucose] were noted in most cases by the end of, or immediately following, the period of asphyxia. The onset, magnitude and reversal of these effects on [glucose] were less predictable than the observed effects on [K(+)].4. There were no significant changes in the [Mg(2+)], [Ca(2+)] or [Na(+)] of any of these fluids. The calculated total osmolarity of the cortical subarachnoid fluid and, to a much lesser extent, of cisternal fluid and plasma, increased during asphyxia mainly as a result of increased [K(+)].5. The results are interpreted as indicative of a rapid release of K(+) from cortical cells during total asphyxia. The (immature) haematoencephalic K(+) transport system becomes saturated and thus K(+) accumulates in the extracellular fluid (e.c.f.) whence it diffuses into adjacent regions of the c.s.f. system.6. The intracellular fluid of apical dendrites must become even more hypertonic than the e.c.f., since these cellular processes are known to swell during asphyxia at the expense of the e.c.f. space. This apparent increase in intracellular osmolarity could be accounted for by the release of normally bound intracellular cations.7. On the basis of our results and review of the relevant literature, the following sequence of events is proposed: the cortex responds to acute physiological stress (asphyxia, overstimulation, chemical or physical irritation, etc.) by releasing intracellularly bound cations (K(+) and possibly Na(+)). The increased intracellular osmolarity results in the absorption of water from the e.c.f. space. Passage of water across the blood-brain barrier is restricted; thus the e.c.f. space of the cortex does not swell, but becomes hyperosmotic. Under these circumstances, swelling of the cortical cells is limited by the volume of e.c.f. available.8. It is proposed that the release of intracellularly bound cations is a result of their displacement from their binding sites by NH(4) (+) which is released to, and recovered from, these cation binding sites by a glutamate-glutamine interconversion.9. It is concluded that the apparent organized ;shutdown' of the cortical cells in response to acute stress may contribute to the relative insensitivity of this area of the brain to permanent histopathological damage.


Interpretation of persistence and effects of polychlorinated biphenyls in birds.


Binding of EDTA, histidine and acetylsalicylic acid to zinc-protein complex in intestinal content, intestinal mucosa and blood plasma.


Inhibition of gastric secretion by acidification of the duodenal bulb before and after transection of the pylorus.


The potency of gastrin and related peptides to enhance the gastric secretory response to sham feeding.


Surgical cleanliness, hospital salubrity, and surgical statistics, historically considered.


Treatment of acute hepatic coma by ex vivo baboon and human liver perfusions.


Ultrastructure and contractile properties of isolated myofibrils and myofilaments from drosophila flight muscle.


Heart block complicating acute bacterial endocarditis.


A technique for time-ordered display of temporal patterns of evoked neuronal unit activity.


Maleylation of bovine colostral IgG and human serum IgG.


Use of reinforced (mylar-backed) cellulose acetate membranes for quantitative radial immunodiffusion.


Stereological methods in the analysis of neuronal parameters in the central nervous system.


Saint Louis encephalitis viral ribonucleic acid replication complex. Pulse-labeled Saint Louis encephalitis viral ribonucleic acid (RNA) is found in the cytoplasm of infected cells associated with a membranous structure which sediments with an average value of 250S. The integrity of the complex is destroyed by detergents and ribonuclease; however, it is stable in ethylenediaminetetraacetic acid (EDTA) which differentiates this structure from cellular polyribosomes. With cultures in which cellular RNA was highly labeled prior to infection, ribosomal RNA could not be demonstrated in the complex isolated from EDTA-sucrose gradients. Single-stranded 43S and the 26S and 20S forms of viral RNA were found in the complex. Viral RNA polymerase activity in sucrose-gradient fractions sedimented in the same region as the fractions which contained the pulse-labeled viral RNA. The polymerase incorporated (3)H-guanosine triphosphate into acid-precipitable material in the absence of added template. It was also found that the replication complex contains viral-specific proteins.


Dominance relationships in mixedly infected Bacillus subtilis. The progeny released from Bacillus subtilis cells mixedly infected with bacteriophages beta22, SP82, and SP02(c1) have been studied at varying multiplicities of infection and orders of addition and with different host strains of the bacterium. In B. subtilis 168, SP02(c1) was subordinate to both SP82 and beta22 and did not yield significant numbers of progeny even when added 5 min before the superior phage. Dominance in mixed infections of beta22 and SP82 was host-dependent. In B. subtilis 168, SP82 was dominant and greatly reduced the yield of beta22 if added simultaneously or before the subordinate partner. However, in the same mixed infection in B. subtilis SB11, beta22 was the dominant phage and totally suppressed the production of SP82 even when added 5 min after the latter.


New deoxyribonucleic acid polymerase induced by Bacillus subtilis bacteriophage PBS2. The deoxyribonucleic acid (DNA) of Bacillus subtilis phage PBS2 has been confirmed to contain uracil instead of thymine. PBS2 phage infection of wild-type cells or DNA polymerase-deficient cells results in an increase in the specific activity of DNA polymerase. This induction of DNA polymerase activity is prevented by actinomycin D and chloramphenicol. In contrast to the major B. subtilis DNA polymerase, which prefers deoxythymidine triphosphate (dTTP) to deoxyuridine triphosphate (dUTP), the DNA polymerase in crude extracts of PBS2-infected cells is equally active whether dTTP or dUTP is employed. This phage-induced polymerase may be responsible for the synthesis of uracil-containing DNA during PBS2 phage infection.


A bacteriophage of bacillus subtilis which forms plaques only at temperatures above 50 C. 3. Inhibition of TSP-1-specific deoxyribonucleic acid synthesis at 37 C and 45 C. The effect of temperature on phage-specific deoxyribonucleic acid (DNA) synthesis was studied in TSP-1-infected Bacillus subtilis. This was facilitated by selectively inhibiting host DNA synthesis with 6-(p-hydroxyphenylazo)-uracil. The results indicated that TSP-1 DNA synthesis did not continue at 37 C and was immediately shut down after transfer to this temperature. Incubation at 45 C greatly reduced TSP-1 DNA synthesis. Phage-specific DNA synthesis could resume at 53 C, however, when the infected culture was returned to 53 C after a 2-min incubation period at 37 C. The results suggest that the inhibition of phage DNA synthesis at 37 C is reversible. Since infected cultures returned to 53 C after 2 min at 37 C could not complete the replicative cycle, the irreversible inhibition of yet another intermediate step was suggested.


Effects of changes in the major carbon source on the fatty acids of Euglena gracilis.


Hydroxylations of carbaryl by soil fungi.


A fetal antigen associated with human neoplasia.


Nutritional requirements of subhuman primates.


Chemical compositions of the hyphal walls of three chromomycosis agents.


Tissue- and species-specific antibodies in antithymocyte globulin.


The planning and organization of a health laboratory service. Fifth report of the WHO Expert Committee on Health Laboratory Services.


Evaluation of food additives. Fifteenth report of the Joint FAO-WHO Expert Committee on Food Additives.


Infants of diabetic mothers. II. Acid-base and electrolyte balance during the first 48 hours after birth.


Transsacral puncture of the arachnoidal sac. An alternative procedure to lumbar puncture.


Intra-ocular pressure and anaesthesia. Direct measurements by needling the anterior chamber in the monkey.


Arthrodesis of the ankle. A follow-up study on 28 patients.


Subtalar arthrodesis. A follow-up study on 22 patients.


Hereditary elliptocytosis and Plasmodium falciparum malaria.


Experimental Schistosoma haematobium infection in vervet monkeys (Ceropithecus aethiops centralis).


Decline in pancreatic insulin release during hemorrhagic shock in the baboon.


Experimental Schistosoma mansoni infections in vervet monkeys (Cercopithecus aethiops centralis).


Comparative studies on the lesions caused by eggs of Schistosoma japonicum and Schistosoma mansoni in livers of albino mice and rhesus monkeys.


Lipolysis after nitroglycerine and its inhibition by propranolol.


Transport and storage of 5-hydroxytryptamine in pancreatic  -cells.


Contributions of heavy and light chains of rabbit immunoglobulin G to antibody activity. I. Binding studies on isolated heavy and light chains.


Multiple pancreatic lipases. Tissue distribution and pattern of accumulation during embryological development.


Comparison of treatment of metastatic gastrointestinal cancer with 5-fluorouracil (5-FU) to a combination of 5-FU with cytosine arabinoside.


Osteogenic sarcoma of the skull: a rare sequela of pituitary irradition.


contribution of Gudden's tegmental nuclei to the reticular activating system.


Chromosome constitution of transitional cell carcinoma of the urinary bladder.


Sensory innervation of the clitoris and the penis in the Macaque.


Sarcosine dehydrogenase deficiency, the enzyme defect in hypersarcosinemia.


Interferon and haemopoietic colony inhibitor responses to poly I-poly C in rabbits and hamsters.


The immunological and physicochemical properties of mitogenic proteins derived from Phaseolus vulgaris.


The separation of different cell classes from lymphoid organs. VI. The effect of osmolarity of gradient media on the density distribution of cells.


Mediastinal fibrosis complicating healed primary histoplasmosis and tuberculosis.


Significance of sign in an S-R separation problem.


Abducens unit behavior in the monkey during vergence movements.


The effects of real and dark light on single unit activity in Macaca mulata.


WHO Expert Committee on Smallpox Eradication. Second report.


Opiates and their alternates for pain and cough relief. Report of a WHO scientific group.


[Properties of the pantothenate transport system in Pseudomonas fluorescens P-2].


On the regulation of the formation of the pantothenate transport system during the growth of Pseudomonas fluorescens P-2.


Effects of simple and complex carbohydrates on serum lipids and atherosclerosis in nonhuman primates.


Progressive protein undernutrition and intestinal enzyme activities in monkeys.


Patterns of insulin response to glucose in protein-calorie malnutrition.


Oral immunization with polyvalent streptomycin-dependent Escherichia coli vaccine. Streptomycin-dependent Escherichia coli serotypes O 111, O 55 and O 26 were used to vaccinate young patas monkeys against these organisms by the oral route. Five administrations of 10(10) cells in 3-day intervals gave almost full protection to the animals against challenge with 9 to 11 minimal infective doses of homologous virulent organisms for at least 1 month, whether the three serotypes were administered singly or simultaneously.


Effect of NO 2  on airborne Venezuelan equine encephalomyelitis virus. Studies were conducted to determine the effect of nitrogen dioxide (NO(2)) on aerosol survival and biological decay rate of Venezulean equine encephalomyelitis (VEE) virus and spores of Bacillus subtilis var. niger. The NO(2) concentrations used in the experiments were 0.5, 5, and 10 ppm at 24 C and 85% RH. The survival of airborne VEE virus disseminated as particles 1 to 5 mum in diameter was significantly influenced by the presence of 5 ppm of NO(2). At this concentration, the biological decay rate increased threefold and the aerosol recovery and aerosol survival of the VEE virus were significantly lower than at 0.5 ppm or in the absence of NO(2). Airborne spores of B. subtilis were not significantly affected by as much as 10 ppm of NO(2).


Comparison of a newly developed latex agglutination test and an immunodiffusion test in the diagnosis of systemic candidiasis. A newly developed latex agglutination (LA) test and a modified immunodiffusion (ID) test were evaluated. The antigen used was a homogenate of Candida albicans. A total of 167 antisera were employed in the evaluation. They included 36 sera from clinically well persons; 78 from patients with various clinical forms of candidiasis; 52 from patients with proven cases of aspergillosis, blastomycosis, coccidioidomycosis, cryptococcosis, histoplasmosis, nocardiosis, paracoccidioidomycosis, sporotrichosis, and tuberculosis; and one serum from a patient with toruloposis. Use of the LA test in conjunction with the ID test permitted the detection of more than 90% of 43 proven candidiasis cases. Of all the heterologous cases and normal human sera tested, LA reactions were noted with 3 of 10 cryptococcosis case specimens, 1 of 9 tuberculosis case specimens, and with the torulopsemia case serum. In contrast, the only heterologous serum reactive in the ID test was that from the patient with torulopsemia. Torulopsis glabrata and C. albicans antisera gave identical reactions in LA and ID tests with T. glabrata or C. albicans antigens. ID tests with selected antigens, however, permitted differentiation of rabbit and human T. glabrata antibody from that of C. albicans antibody. Six different precipitins were recognized with the C. albicans antigens. The occurrence of multiple precipitin lines and high LA titers was suggestive of severe candidiasis. The LA test, in contrast to the ID test, appeared to have prognostic value. Together, the LA and ID tests provided a simple, rapid, and accurate means of detecting and monitoring infections by species of Candida.


Infected surface wound: an experimental model and a method for the quantitation of bacteria in infected tissues. Methods for the quantitation of bacteria in infected tissues must be rigidly standardized to insure uniformity of results. In this communication we report on a laboratory animal model for the study of surface wound infection and the development of a standardized method for the quantitative estimation of bacteria in infected surface wound tissue by mechanical tissue homogenization and serial dilution. Parallel comparative studies demonstrated that a moist-swab sampling procedure detected only 10% of the bacteria recoverable by a surface-wash procedure. Either tissue homogenization or surface-wash procedures recovered significantly more bacteria from contaminated surface wounds than were obtained by surface-swab sampling techniques.


Mycoplasma: growth precipitation as a serodiagnostic method. A serological test is described which is designated the growth precipitation test and is based on the release of diffusible compounds from mycoplasma strains. During growth of mycoplasmas on solid substrates, precipitation lines are formed with antibodies from homologous antisera independent of heat-labile serum factors. The application of the test as a serodiagnostic tool was investigated by performing a complete serological cross-test with 10 mycoplasma strains. The test was compared with the growth inhibition test, the complement fixation test, and the indirect hemagglutination test. It is demonstrated that the growth precipitation test has a good degree of specificity. The species and subspecies used here showed cross-reactions between serologically closely related strains, but these heterologous reactions occurred only after longer incubation than the homologous reactions.


Sporostatic and sporocidal properties of aqueous formaldehyde. Aqueous formaldehyde is shown to exert both sporostatic and sporocidal effects on Bacillus subtilis spores. The sporostatic effect is a result of the reversible inhibition of spore germination occasioned by aqueous formaldehyde; the sporocidal effect is due to temperature-dependent inactivation of these spores in aqueous formaldehyde. The physicochemical state of formaldehyde in solution provides a framework with which to interpret both the sporostatic and sporocidal properties of aqueous formaldehyde.


Failure to detect cell-associated enterotoxin B in Staphylococcus aureus by immunofluorescence. Enterotoxin B-producing and -nonproducing Staphylococcus aureus strains showed cell fluorescence when tested with fluoresceinisothiocyanate-labeled rabbit anti-enterotoxin B globulin, probably as a result of a protein A-immunoglobulin G (Ig G) interaction. No cell-bound enterotoxin B could be detected by immunofluorescence using F(ab(1))(2)-fragments of anti-enterotoxin B globulin. However, soluble enterotoxin B could be estimated by immunofluorescence. Approximately 1,000-fold more enterotoxin B was detected by immunodiffusion as an extracellular product in the media than could be detected in the cell fraction. The results show that intact Ig G is not suitable for the detection of antigens other than protein A on the cell surface of S. aureus in conventional immunofluorescence. For such purposes, the use of F(ab(1))(2)-fragments of Ig G is recommended.


Comparison of the antigenicity of phenol and ethylenediaminetetraacetate complexes isolated from cell walls of Salmonella enteritidis. Cell walls of Salmonella enteritidis were extracted with phenol and with ethylenediaminetetraacetate (EDTA). Phenol extracts were consistently more antigenic in chickens than EDTA extracts. Differences in the action of EDTA and phenol upon bacterial cell walls were demonstrated by the use of radioisotopes.


Salicylic acid biosynthesis and its control in Mycobacterium smegmatis.


Identification and aflatoxin production of molds isolated from country cured hams. Of 562 molds isolated from country cured hams, 403 isolates were of the genus Penicillium, 121 were Aspergillus, and 36 were Cladosporium, Alternaria, and other genera.


Synthesis of biotin-vitamers from pimelic acid and coenzyme A by cell-free extracts of various bacteria.


Lipoic acid dependency of human branched chain  -ketoacid oxidase.


Treatment of soft-tissue injuries by pulsed electrical energy. Twenty pairs of patients matched for sex, age, weight, and degree of trauma were treated either with an active Diapulse machine emitting pulsed, high-frequency electrical energy or with a non-active placebo machine. They were examined for swelling, pain, and disability before treatment and after three days under double-blind conditions. The results show that the pulsed, high-frequency electrical treatment has a definite biological effect on recently injured soft tissues, especially in the reduction of pain and disability.


Craniopharyngiomas.

Craniopharyngiomas.


Myocardial and skeletal muscle concentrations of digoxin in patients on long-term therapy. The digoxin content was measured in samples of left ventricular papillary muscle, skeletal muscle, and plasma obtained during mitral valve replacement from eight patients on maintenance treatment with the drug. The content in papillary muscle ranged from 15.5 to 132 ng/g (mean 77.7) and in skeletal muscle from 7.5 to 23 ng/g (mean 11.3). The ratio of myocardial digoxin concentration to plasma concentration varied between patients from 39:1 to 155:1. No simple relationship exists between plasma levels of digoxin and its concentration in the heart muscle, but total myocardial concentration may not accurately reflect therapeutic activity.


Dialysance of molecules of different size in reused Kiil, Ab-Gambro, and Rhône-Poulenc dialysers. Dialysances of urea, creatinine, ethylenediamine tetraacetic acid, and ouabain were determined in three different flat-bed dialysers during three successive uses on 15 patients. There were no untoward reactions. A significant decrease in dialysing efficiency was observed only with the Ab-Gambro dialyser, in which the dialysance of the small molecules decreased more than that of the large. This is thought to result from blood deposits on the membrane surface inhibiting exchange.


Rh-sensitization and third stage of labour.


Antiviral activity of aqueous clam (Mya arenaria) extract on amphibian virus (LT-1).


Abortive infection of hamster embryo cells with simian virus type 15 (SV15). I. "Helper" activity for adenovirus-associated virus type 1.


Degradation of rutin by Aspergillus flavus. Studies on specificity, inhibition, and possible reaction mechanism of quercetinase.


Copper pathochemistry in vitiligo.

Copper pathochemistry in vitiligo.


An improved Ouchterlony plate.

An improved Ouchterlony plate.


A simple and rapid method for the measurement of plasma ultrafiltrable and ionized calcium.


Automatic measurement of some mucosal carbohydrases.


Serum immunoglobulin levels in white patients with sickle cell disease.


Isolation of a membrane protein with a low molecular weight derived from the human erythrocytes: the chemical properties and the investigation of the Rh antigenicity with the Schultz Dale test.


Osmotic stability and sodium and potassium content of L-forms of Streptococcus faecalis. A stable L-form of Streptococcus faecalis (T(53)) was transferred in media containing decreasing concentrations of sucrose until it grew in medium without added osmotic stabilizer. This L-form (designated T(53I)) was compared with T(53) from which it was derived. The survival of these two L-forms suspended at different osmolalities showed that maximal survival for T(53I) was 350 to 400 milliosmolal and for T(53) was 900 to 1,000 milliosmolal. Both peaks were at the osmolality of their growth media. Measurement of intracellular potassium and sodium showed that the concentration of these ions was reduced in T(53I).


Ribulose diphosphate carboxylase from autotrophic microorganisms. Thiobacillus denitrificans was grown anaerobically with nitrate as an acceptor in both sterile and nonsterile media. Ribulose diphosphate carboxylase was stable throughout the exponential growth phase and declined slowly only after cells reached the stationary phase. Reversible inactivation of the carboxylase occurred in extracts as a result of bicarbonate omission. The enzyme was purified 32-fold with excellent recovery of a preparation which was 50 to 60% pure by the criterion of polyacrylamide gel electrophoresis. This purified preparation catalyzed the fixation of 1.25 mumoles of CO(2) per min per mg of protein at pH 8.1 and 30 C, and the molecular weight of ribulose diphosphate carboxylase was approximately 350,000 daltons. A striking biphasic time course of CO(2) fixation that was independent of protein and ribulose diphosphate concentration was observed. The optimal pH of the enzyme assay was fairly broad, ranging from 7 to 8.2. Kinetic dependence upon bicarbonate, ribulose diphosphate, and Mg(2+) was characterized and indicated that bicarbonate and Mg(2+) must combine with enzyme prior to addition of ribulose diphosphate. Antiserum to ribulose diphosphate carboxylase from Hydrogenomonas eutropha was only slightly inhibitory when added to the enzyme from T. denitrificans, and the mixture did not precipitate. Cyanide (4 x 10(-5)m) gave 61% inhibition of the enzyme from T. denitrificans. Ribulose diphosphate carboxylase in extracts of H. eutropha, H. facilis, Chromatium D, Rhodospirillum rubrum, and Chlorella pyrenoidosa were also inhibited to varying extents by cyanide and antiserum to the H. eutropha enzyme.


Comparison of deoxyribonucleic acid uptake and marker integration in bacilli and protoplasts of Bacillus subtilis. trp(+)his(-) donor deoxyribonucleic acid (DNA) was added to highly competent trp(-)his(+) recipient bacilli and to protoplasts prepared from these bacilli, and the cell-DNA complexes were incubated for 30 min. The complexes were then washed and lysed, and their DNA was analyzed on a trp(-)his(-) strain for the donor marker trp(+), the resident marker his(+), and for the recombinant trp(+)his(+) combination. The extracts of the bacillary complexes contained a normal percentage of donor markers (0.1-0.2%), and the number of trp(+)his(+) doubles (20% of all trp(+) transformants) indicated that the donor DNA had become integrated into the resident genomes. The protoplast complexes contained 10 to 1,000 times fewer donor markers and almost no recombinants. This indicated that, in protoplasts, marker uptake was minimal and recombination was absent. Uptake was also measured with (3)H-labeled DNA. On the average, protoplasts took up one-fiftieth as much DNA as bacilli. It was concluded that, probably, protoplasts took up no DNA at all, that there were no DNA affinity sites on the surface of the protoplasts, and that the residual marker and radioactivity uptake was due to imperfections in the experimental system. The data and conclusions differed sharply from earlier ones of Hirokawa and Ikeda despite the fact that the techniques of these authors were followed in repeat experiments.


Detection of cholesterol in cell membranes by use of bacterial toxins. A method is described for the detection of cholesterol in membranes from erythrocytes, mycoplasmas, and bacterial cells by a ferritin-labeling technique. Membranes treated with cereolysin, a bacterial hemolysin which specifically binds to cholesterol, and then treated with ferritin-antitetanolysin, were specifically ferritin-labeled for cholesterol. A similar antigen-antibody system, streptolysin O-ferritin-antistreptolysin, was also used successfully with erythrocyte membranes. There was an uneven distribution of ferritin in erythrocyte membranes suggesting that the distribution of cholesterol may not be entirely random. Mycoplasma gallisepticum was intensely labeled, but Acholeplasma laidlawii with or without cholesterol in the membranes was not labeled, suggesting an unusual location for cholesterol in A. laidlawii membranes. As controls, two of three species of bacterial membranes lacking cholesterol were not ferritin-labeled.


The uropod-bearing lymphocyte of the guinea pig. Evidence for thymic origin. In this study, the frequency of uropod formation and the type of lymphocyte bearing the uropod was investigated in various guinea pig lymphocyte populations. Without prior in vitro stimulation, almost 40% of peritoneal exudate lymphocytes (PELS) form uropods, while thymocytes and lymph node cells form far fewer. Subsequent stimulation in vitro with purified protein derivative demonstrated that there is an association between antigen reactivity and frequency of uropod formation in these populations. The ultrastructure of these uropods is identical to that described for human lymphocytes stimulated with phytohemagglutinin. In the populations studied, all the lymphocytes forming uropods lack easily detectable surface membrane immunoglobulin and are therefore most likely thymus-derived or T lymphocytes.


Leukotactic factors elaborated by virus-infected tissues. Infection of chick embryos wih either Newcastle disease virus or mumps virus and infection of BGM cell cultures with mumps virus result in the elaboration of chemotactic activity for neutrophils and macrophages. These factors cannot be found in lysates of uninfected cells. They do not appear to be associated with the viral particles per se, but rather are present in virus-free supernates from infected fluids. Ultracentrifugal studies of the neutrophil chemotactic activity in allantoic fluid of embryos infected with the two different viruses indicate a similar biphasic distribution of activity, while fluid from the mammalian cell cultures shows a single zone of leukotactic activity, further suggesting that the infected cell, rather than the virus, is responsible for the leukotactic activity. Virus-infected cells also release a substance(s) which is itself not leukotactic but which can interact with human C3 or C5 to generate such activity. This leukotactic factor-generating substance is similar to that reported in another virus-infected cell system. It is postulated that the leukotactic factors elaborated as a result of virus infection of cells may play a protective role in vivo.


Requirement of thymus (T) lymphocytes for resistance to listeriosis. Spleen cells of mice infected with Listeria monocytogenes were adoptively transferred to normal mice. Such lymphocytes conferred resistance to a lethal challenge with Listeria. Hyperimmunization of the donor reduces the number of cells necessary to transfer effective immunity. Such spleen cells if treated with anti-theta serum do not transfer resistance to Listeria. Hence, thymus (T) lymphocytes are involved in the resistance to infection with the facultative intracellular bacteria L. monocytogenes.


Active suppression of immunoglobulin allotype synthesis. I. Chronic suppression after perinatal exposure to maternal antibody to paternal allotype in (SJL x BALB-c)F 1  mice. Long-term (chronic) allotype suppression, previously reported only in rabbits, is shown here to occur in at least one strain combination of mice as well. Close to 50% of the offspring of SJL (Ig(b)) males mated to BALB/c (Ig(a)) females immunized against the paternal allotype were found to be suppressed for Ig-1b (gammaG(2a)) at 6 months of age. These mice are called "chronically" suppressed. The percentage of offspring in this strain combination suppressed for the paternal allotype at 8 wk of age is the same as that seen in an earlier strain combination tested, [(C57 x BALB/c)F(1)], in which all mice recover from suppression by 10-12 wk. After 8 wk, two distinct patterns of long-term (chronic) suppression emerge in (SJL x BALB/c)F(1) mice: a small number of these mice never produce detectable amounts of Ig-1b throughout their lives, while the majority produce detectable Ig-1b sporadically, sometimes over a period of several weeks, the level of which eventually falls below detectability. Attempts to "cure" suppression by destroying the existent lymphoid population and forcing endogenous repopulation in chronically suppressed animals were unsuccessful. Furthermore, attempts to restore Ig-1b production by injection of cells from syngeneic Ig(a)/Ig(b) donors into irradiated, chronically suppressed recipients were also unsuccessful, although the same cell inocula, when injected into irradiated BALB/c (Ig(a)/Ig(a)) mice produced high levels of gamma globulin carrying the allotype. These results suggest that long-term allotype suppression resulting from perinatal exposure of offspring to specific anti-allotype antibody (anti-Ig-1b), is not due merely to an absence of Ig-1b-producing cells or their progenitors, but appears to be an active process, which dominates physiologically over normal production.


Active suppression of immunoglobulin allotype synthesis. II. Transfer of suppressing factor with spleen cells. The mechanism of chronic allotype suppression in (SJL x BALB/c)F(1) mice has been investigated by means of cell transfer studies. These mice are phenotypically negative for serum Ig-1b, the paternal allotype determinant on gammaG(2a) immunoglubulin, as a result of perinatal exposure to maternal anti-Ig-1b. When spleen or bone marrow (B) cells from suppressed mice were injected into irradiated BALB/c "indicator" hosts, detectable levels of Ig-1b were demonstrated in the sera of a majority of the recipients early after transfer. These results indicate that Ig-1b-producing cells or their precursors are present in the lymphoid tissues of suppressed mice, even though they are not expressed. Within 5-7 wk, it was no longer possible to detect Ig-1b in the sera of these hosts, although cells producing another paternal allotype (Ig-4b) were shown to persist. Control BALB/c mice, injected with spleen and B cells from normal mice, continued to produce high levels of immunoglobulin carrying this allotype. The disappearance of serum, Ig-1b occurred most frequently in the recipients of suppressed spleen cells. Similar results were obtained using a mixture of spleen cells from normal and suppressed mice. Ig-1b production in the recipient mice ceased within a few weeks, even though the majority of cells in the mixture were obtained from normal (nonsuppressed) donors. The data are interpreted as evidence that chronic allotype suppression in mice is actively maintained by cells which are resident in the lymphoid tissues, splenic cells being the most effective. These cells are capable of proliferating in a new host and exerting their suppressive influence on Ig-1b-producing cells and/or their precursors.


Further evidence concerning the autoantigenic status of the trophoblast. Heterologous antisera were raised by inoculation of rabbits with fairly pure suspensions of trophoblast, lymphoid, fetal (excluding placental components), epidermal, decidual, and renal cells from Fischer rats. After absorption of hemagglutinins, these antisera were assayed for abortifacient activity by intramuscular inoculation into time-mated Fischer females. The anti-trophoblast serum aborted all the recipients, but had some nonspecific activity in that it caused the deaths of 12% of them. Anti-lymphocyte serum was more toxic and less potent as an abortifacient. None of the other sera harmed either the mothers or their fetuses. However, the anti-kidney cell serum caused kidney lesions. Absorption of the anti-trophoblast serum with lymphoid cells completely removed its toxicity for the pregnant females without perceptible impairment of its abortifacient activity. However, the latter could be removed by absorption with trophoblast cells. (a) The lymphocyte-absorbed anti-trophoblast serum was equally effective in aborting rats over the range 7-18 days postconception; (b) a single injection of 1 ml was sufficient in most cases, and (c) it was equally efficacious in terminating gestation in rats irrespective of their genetic constitution, and (d) its effect was highly species-specific. These findings support the premise that a unique antigen (or antigens), of possible clinical significance, is associated with trophoblast cells.


Immune status of mice tolerant of living cells. II. Continuous presence and nature of facilitation-enhancing antibodies in tolerant animals. CBA mice were rendered highly tolerant to A/Jax cells by neonatal intravenous injections of (CBA x A)F(1) spleen cells. The high degree of tolerance was ascertained by the absence of circulating antibodies detected in the sera by the usual tests and by the perfect state of A skin grafts during all the experiments. Tolerant sera (sera from tolerant animals) were studied at three periods of tolerance: before skin test grafting, from 2 to 11 wk after grafting, and at time of sacrifice at almost 6 months of age. The tolerant sera were shown to have specific facilitation-enhancing properties promoting the take and growth of A/Jax sarcoma (SaI and /Sa 15091a grafted on normal CBA mice. These properties were present throughout the duration of the experiments, showing that they were not the result of a beginning interruption of tolerance. The tolerant sera, although lacking the usual serological properties (hemagglutination, hemolysis, cytotoxicity, passive cutaneous anaphylaxis) had, however, specific synergistic hemagglutinating properties (increasing the hemagglutinating titer of a reference immune serum). Antibodies giving direct specific hemagglutination could be extracted from spleens of 20% of highly tolerant mice. The tolerant sera were also found to contain more IgG1 and more IgA than normal sera while they contained normal quantities of the complement-fixing immunoglobulins IgG2 and IgM. Fractionation of tolerant sera on DEAE chromatography column confirmed the data concerning immunoglobulin classes and demonstrated direct specific serological activities undetected in unfractionated sera: a weak hemolysis in the most cationic fractions and a weak hemagglutination in the middle fractions. Synergistic hemagglutination, detected in unfractionated serum, was localized in fast anionic fractions containing high IgA concentration, along with facilitation-enhancing activity, thus confirming a link suggested previously between these three properties. The relation between immunological tolerance and facilitating antibodies was discussed in the light of the fact that antibodies, possibly of a particular class continuously present at low dose in the sera of highly tolerant animals, are able to transfer (at least partly) this state of tolerance provided a sensitive test system is utilized.


Comparison of the internal proteins of the T-even bacteriophages.


Preface--series of papers relating reproductive activity to selected organosiloxane chemicals.


Biochemical effects of repeated administration of P,p'-DDT on the squirrel monkey.


Effect of ADH, aldosterone, ouabain, and amiloride on toad bladder epithelial cells.


Acid-base behavior of the kidney in maleate-treated rats.


Digesta flow through the rabbit large intestine.


Calcium content and exchange in amphibian skin and its isolated epithelium.


Turnover rate of orosomucoid in the dog with sterile abscess.


Alkaline proteinases of the genus Aspergillus.


Susceptibility of calves to bovine syncytial virus given by different inoculation routes.


Comparative physicochemical studies of human alpha-lactalbumin and human lysozyme.


Structural studies on bovine immunoglobulin M.


Isolation and partial electrophoretic characterization of total protein from non-sheared rat liver chromatin.


The isolation of an EDTA-soluble phosphoprotein from mineralizing bovine dentin.


Electroencephalographic studies in isolation-raised monkeys with behavioral impairment.


Control of behavioral symptoms in patients with long-term illness.


Multiple forms of staphylococcal alpha-toxin.


Thyroid function in the prenatal and neonatal bovine.


Cetrimide-nalidixic acid agar as a selective medium for Speudomonas aeruginosa.


Taxonomy of mycobacteria studies by polyacrylamide-gel electrophoresis of cell proteins.


The effect of 1-hydroxyphenazine and pyocyanin from Pseudomonas aeruginosa on mammalian cell respiration.


Life-saving removal of a non-functioning tuberculous kidney: a case report.


Violence and the brain.

Violence and the brain.


Statistical and mathematical analysis of clinical and sub-clinical paroxysms petit mal.


Thalassaemia major with complete suppression of HbA production in a Ghanaian girl.


Endocrine studies in a case of congenital (erythroid) hypoplastic anaemia.


Fibrinolysis in myelomatosis.

Fibrinolysis in myelomatosis.


L-asparaginase effect on the erythrocyte-immunoglobulin binding in acquired hemolytic anemia.


Prophylactic treatment of meningeal leukemia in children by intrathecal methotrexate.


The urinary excretion of haemoglobin in the isolated normothermic-perfused dog kidney.


Supracortical fluid: a monitor of albumin exchange in normal and injured brain.


Interaural stimulation: effects on the Q value of tuning curves and post-stimulus time histograms of cat auditory cortex neurons.


Effect of prostaglandins and their inhibitors on osmotic water flow in the toad bladder.


Effects of mesencephalic reticular formation lesions on optokinetic nystagmus.


Effects of sub-lethal heat-shock,  -alanine, and L-alanine on germination and subsequent destruction of Bacillus spores by pasteurization.


Toxic effect and action of dead sperm on diluted bovine semen.


Current status of the Milko-Tester.

Current status of the Milko-Tester.


The free amino acid pool of the haemocytes of Carcinus maenas (L.).


Transmission of four Central American strains of Plasmodium vivax from monkey to man.


Role of Haemogamasus reidi (Acari: Mesostigmata) in the life cycle of the gray squirrel protozoan, Hepatozoon griseisciuri (Sporozoa: Haemogregarinidae).


Effect of 6-propylthiouracil-induced hypothyroidism in mice experimentally infected with Trypanosoma cruzi.


Extending the scope of nursing practice. A report of the Secretary's Committee to study extended roles for nurses.


Cor leopardum.

Cor leopardum.


Rectal and pharyngeal gonorrhea in homosexual men.


Lead poisoning in dogs: analysis of blood, urine, hair, and liver for lead.


Precipitins in serums from sheep infected with Aspergillus fumigatus.


Distribution of antibodies to transmissible gastroenteritis virus in serum and milk of sows: isolation and identification of the immunoglobulin classes of swine serum and milk.


A critical evaluation of the histochemical methods for carbonic anhydrase.


Immunocytochemical localization of the extracellular polysaccharide alginic acid in the brown seaweed, Fucus distichus.


A new serum factor in normal rabbits. VII. Action of immunosuppressive drugs in neonatal rabbits.


Detection of serum-hepatitis antigen by charcoal particle agglutination-inhibition.


Immunoglobulin content and antibody activity in urine in experimental urinary tract infection.


Assay of IgG and other human plasma proteins by quantitative inhibition of passive hemagglutination.


A genetic polymorphism of Peromyscus 7S  1  globulins: detection by differences in electrophoretic mobility.


A radioactive antigen-binding assay for Neisseria meningitidis polysaccharide antibody.


Quantitative method for determining human  G allotype antigens (Gm). II. Differences in Gm gene expression for  G1 and  G3 H chains in sera.


Plexiform neurofibromas in childhood.

Plexiform neurofibromas in childhood.


Evaluation of different nitrogen sources in high-calorie parenteral therapy.


Anorexia nervosa associated with pregnancy.


Two physiological forms of human retinol binding protein.


The effects of common salts on the Euglena gracilis bioassay of vitamin B 12 .


Antigenicity of double-stranded ribonucleic acids including poly I:C.


Urinary excretion of progesterone metabolites in pregnant rhesus monkeys.


The alteration of blood group antigens by carbodiimide.


Chorionic gonadotropin secretion during pregnancy in the chimpanzee (Pan troglodyte).


Bactericidal antibody assay using  14 C-labeled Neisseria meningitidis.


Identification and purification of a new non-heme, non-ferritin iron protein.


Pesticide residues and polychlorinated biphenyl levels in diets, urine, and fecal matter of preadolescent girls.


Lowered glucose tolerance in the sand rat (Psammonys obesus) resulting from esophageal intubation.


Cortical lesions and auditory discrimination.


Prospects for medicine in the 1970s.

Prospects for medicine in the 1970s.


Salivary IgA and infection in children with atopy.


Raised free thyroxine values in patients with familial elevation of thyroxine-binding globulin.


Mock malignancies in the urinary tract.

Mock malignancies in the urinary tract.


Growth pattern in a boy with thyroxine-binding globulin deficiency.


Whole-body scanning and  131 I therapy in the management of thyroid carcinoma.


Hyperbaric oxygen as an adjuvant to megavoltage radiotherapy.


The histogenesis of immunologically committed lymphocytes.


Characterization of autolysed membranes of Bacillus cereus. Characterization in membrane biosynthesis and binding of hemes to proteins of membrane subfraction L10P.


Changes in the fluorescence of atebrin and of anilino-naphthalene sulfonate reflecting two different light-induced processes in Rhodospirillum rubrum chromatophores.


Response of monkeys to immunization with cholera toxoid, toxin, and vaccine: reversion of cholera toxoid.


The rectal culture as a test of cure of gonorrhea in the female.


Immunogenicity of the group A and group C meningococcal polysaccharides in children.


Studies on the macromolecular and antigenic composition of rabbit seminal plasma.


Effect of LH, FSH and their antisera on gestation in the hamster (Mesocricetus auratus).


Effective thyroid ratio (ETR)--a new test of thyroid function.


Dissociated deficiency of cellular immunological function, cryptococcal meningitis and acute myeloid leukaemia.


Search for Ferris's fecal antigen and Australia antigen in stool samples obtained during a large outbreak of infectious hepatitis.


Fasting acid output and plasma gastrin in conscious monkeys.


The effects of dietary alterations on 3-methyl-4-methylaminoazobenzene N-demethylase activity.


Specific immunofluorescence test for detection of herpesvirus antigen in cells of the Lucke renal adenocarcinoma.


The effects of various levels of dietary protein on retinoic acid-induced teratogenicity in rats.


Effects of methylmercury on prenatal development in mice.


The teratogenic activity of a thalidomide analogue, EM 12  in rabbits, rats, and monkeys.


Effects of ergot alkaloids (Hydergine) on cerebral haemodynamics in man.


Investigation of wasp venom: antigenic relationship.


Cellular and humoral sensitivity to gluten fractions in patients with treated nontropical sprue.


A rapid instrumental method for the determination of dextran in urine.


A vascular cause for aganglionic bowel. A new hypothesis.


Intracellular localization of tyrosinase inhibitor in amelanotic and melanotic malignant melanoma.


Survival of gonococci outside the body.

Survival of gonococci outside the body.


Maximum aerobic power in relation to body composition in healthy, sedentary adults.


Plasminogen- 125 I responses in dogs to a single injection of urokinase and typhoid vaccine and to vascular injury. In vivo plasminogen responses to various stimuli were studied. Plasminogen-(125)I was prepared and used first for metabolic studies of plasminogen in control dogs. The average results were: the plasma plasminogen, 29.3+/-4.1 (SD) mg/kg; the interstitial plasminogen, 8.79+/-4.47 (SD) mg/kg; the half-life of plasma plasminogen-(125)I, 2.81+/-0.24 (SD) days; the fractional direct catabolic rate of plasminogen (j(3)), 0.295 day(-1); and the catabolic (synthetic) rate of plasminogen, 8.61+/-1.35 (SD) mg/kg per day. Studies were then made of the plasminogen-(125)I responses in dogs to a single injection of urokinase (A) and typhoid vaccine (B), and to vascular injury (C), which was produced by the damage of venous endothelium by a phenol injection. Effects of heparin were also studied in dogs given the phenol injection (D). Disc electrophoretic analysis of plasma showed generation of plasmin-(125)I in all except the control experiments. The duration of plasmin-(125)I generation was about 6 hr in A, 6 hr in B, and at least 5 days in C. Heparinization (D) shortened the duration of generation to about 6 hr. For further quantitative analysis of the tracer data, a model for coexistent plasminogen-(125)I and plasmin-(125)I was proposed and validated, from which some new analytical methods were derived. Using these methods, the average fractional rate of plasmin-(125)I generation from plasminogen-(125)I (j(4)) was 0.41 day(-1) in A, 0.30 day(-1) in B, 0.324 day(-1) in C, and 0.382 day(-1) in D. Further mathematical consideration showed that j(3) was zero at least in C during plasmin generation. Plasminogen synthesis was unchanged in all experiments. The average fractional breakdown rate of plasmin-(125)I (j(5)) in A, B, C, and D was 1.19, 1.13, 1.35, and 1.11 day(-1), respectively, and were closely similar. These results indicate that under normal conditions a major portion of plasminogen is directly catabolized without the formation of plasmin, but that significant amounts of plasmin were generated under the conditions described, that the normal process of direct breakdown of plasminogen is abolished during plasmin generation at least in C, and that the potential value of j(5) determination should be further explored.


Effects of prostaglandin F 2   on some reproductive processes of hamsters and rats.


Biochemical characterization of mutants affecting the metabolism of  -alanine in Drosophila.


The relation between cholesterol absorption and cholesterol synthesis in the baboon. To determine the relation between cholesterol absorption, total endogenous cholesterol synthesis, and hepatic cholesterol synthesis in a primate, cholesterol synthesis has been studied in biopsies of liver and ileum from normal baboons fed varying amounts of cholesterol and in biopsies of liver from baboons that had been subjected to ileal diversion. In addition, total cholesterol production rates, cholesterol absorption, and total endogenous cholesterol synthesis have been measured in these animals by a double isotope technique in which the animals were given a single injection of cholesterol-4-(14)C and fed constant amounts of cholesterol-1,2-(3)H for 4 months. From these studies, it has been concluded that on a low cholesterol intake cholesterol synthesis in the liver accounts for about three-fourths of total endogenous cholesterol production. The feeding of cholesterol produces complete inhibition of hepatic synthesis in the normal animal only when absorption approximates the amount synthesized by the liver when no cholesterol is fed, e.g., 400-500 mg/day. Finally, the intestine, which does not possess complete negative feedback control of cholesterol synthesis when cholesterol is fed, may be a significant site of nonhepatic cholesterol synthesis in these animals. In studies of four baboons subjected to ileal diversion, it was concluded that the regulation of cholesterol synthesis is distinctly different when the enterohepatic circulation is interrupted. These animals did not exhibit negative feedback of hepatic cholesterol synthesis when cholesterol was fed, despite the fact that cholesterol absorption approximated that of normal animals fed similar diets. The inference has been drawn that bile acids may be involved directly or indirectly in the regulation of hepatic cholesterol synthesis in this species or that the ileum itself may modulate the hepatic negative feedback.


Renewal of catalase activity in Drosophila following treatment with 3-amino-1,2,4-triazole.


The incidence of milk precipitins in pediatric patients at Charity Hospital in New Orleans.


Polychlorinated biphenyl residues in human plasma expose a major urban pollution problem.


Infection of chick embryo tracheal organ cultures with influenza A2 (Hong Kong) virus. I. Cytopathology, histopathology, immunofluorescence, hemadsorption, and titration of the released infectious progeny virus.


Animal behaviour in intensive systems and its relationship to disease and production.


Cell culture adaptation and propagation of a reovirus-like agent of calf diarrhea from a field outbreak in Nebraska.


Adherence chromatography of rat bone marrow cells.


How aspirin works.

How aspirin works.


Decalcified allogenic bone grafting.

Decalcified allogenic bone grafting.


The oral-antral problem.

The oral-antral problem.


Epilepsy in childhood.

Epilepsy in childhood.


Spinal Pseudomonas chondro-osteomyelitis in heroin users.


The place and importance of experimental animal in medicine today. Physiology.


Toxicology and drug development.

Toxicology and drug development.


The place and importance of the experimental animal in medicine today. Nutrition.


Diverticulosis of the terminal ileum and its complications.


Stabilization of capsid structure and enhancement of immunogenicity of cucumber mosaic virus (Q strain) by formaldehyde.


Comparative study of thermal inactivation of phage phi 80 and lambda.


Seed transmissibility of two strains of barley stripe mosaic virus.


Replication of viral DNA in SPO1-infected Bacillus subtilis. I. Replicative intermediates.


Replication of viral DNA in SPO1-infected Bacillus subtilis. II. DNA maturation during abortive infection.


Leaf ontogeny and virus replication in Brassica pekinensis infected with turnip yellow mosaic virus.


The eye in malignant hypertension.

The eye in malignant hypertension.


Protein composition of coronavirus OC 43.


Induction of localized necrotic lesions by actinomycin D on leaves systemically infected with tobacco mosaic virus.


Purification and properties of cucumber necrosis virus and a smaller top component.


Inactivation of interferon: the effect of protonation.


Detection of acidic polysaccharides in gels by DEAE-dextran.


A methodology for analysis of tissue sulfhydryl components.


Influence of dextran and polyethylene glycol on sensitivity of two-dimensional immunoelectrophoresis and electroimmunodiffusion.


The influence of Listeria monocytogenes on the primary and secondary response in mice.


Quantitative analysis of IgG, IgA and IgM immunoglobulins in lymphatic leukemia and lymphosarcoma.


Cytotoxic properties of nitro derivatives of 9-aminoacridine in cultures of Euglena gracilis.


Behavior of immunoglobulins in malignant lymphogranulomatosis and thymoma.


The use of rhesus monkeys to study biliary secretion with an intact enterohepatic circulation.


A mutant sex factor of Pseudomonas aeruginosa.


An investigation of the components of Segregation-Distorter systems in Drosophila melanogaster. The problems of the Segregation-Distorter system are approached and unified in terms of an orthodox quantitative genetic system. It is shown that the SD system involves positive and negative modifier genes of varying strengths, that there is no major Stabiliser gene, and that the Activator gene is not unique. It is further argued that the SD phenomenon is mainly male limited, with "female effects" being of small magnitude.


A study of spontaneous mutation rates at ten loci detectable by starch gel electrophoresis in Drosophila melanogaster. Spontaneous mutation rates at ten allozyme loci on chromosomes II and III of Drosophila melanogaster were studied. Over the three and a half years study, one alpha-GPD mutation and two different IDH mutations were obtained. The alpha-GPD mutation was inherited in the Mendelian fashion, as expected. The two IDH mutations were peculiar in that the band of new types appeared only in females. In males, only the original bands were stained, and the positions where mutant alleles' bands should be present were blank. Both IDH mutant homozygotes appeared as null allele homozygotes, while in females clear-cut single bands were present.-The rates of spontaneous mutation varied greatly. Eight loci studied (MDH, ADH, EST-6, APH, EST-C, ODH, XDH, AO) did not give any germ-line mutation. The average germ-line mutation rate over all ten loci was estimated at 4.5 x 10(-6). This rate is considerably smaller than that for sex-linked recessive visible mutations (Muller, Valencia and Valencia 1950), but it is somewhat less than autosomal recessive visible mutations (Glass and Ritterhoff 1956).


Genetic change of recombination value in Drosophila melanogaster. I. Artificial selection for high and low recombination and some properties of recombination-modifying genes. A high and low selection line were formed by individual selection of females on the basis of their recombination. In the high line, recombination between Gl and Sb was increased from 14.8 percent to about 30 percent in twelve generations, when a plateau was apparently reached. Realized heritability was 0.12. The absence of a response to selection for low recombination is attributed mainly to genetic random drift, and partially to directional dominance and directional gene frequencies. Natural selection was found to act against increases of recombination above a level of about twenty percent in the measured interval. High recombination tended to be recessive to low recombination. In both selected lines and unselected stocks, intervals proximal to the centromere tended to have a higher recombination variance than distal intervals.


Genetic change of recobination value in Drosophila melanogaster. II. Simulated natural selection. Selection of Gl-Sb coupling heterozygotes was carried out for more than one hundred generations commencing with six independent lines drawn from a common base population. Population sizes were eight, sixteen and forty-eight parents per generation. The effect of natural selection on recombination value was measured by sampling and testing females at varying intervals of time. There was a significant reduction in percentage recombination between Gl and Sb from fifteen to a level between five and ten in four out of six of the original lines. In most cases this reduction occurred rather rapidly after the initiation of the experiment. In the remaining two lines there was no significant decrease in recombination value; there was, however, a significant increase in at least one subline of this group. The rapid rate of change of recombination value is most readily explained by the presence of a recombination modifying gene which is linked to the modified region. Genetic random drift was again shown to have an important effect on changes in recombination value in small populations. High recombination was almost completely recessive to low recombination in the one case examined. Lethal genes were fixed in sheltered regions of unmarked third chromosomes in five lines or sublines. These results are discussed in relation to the mode of development of permanent heterozygosity in some species of plants.


Experimentally determined, two locus fitnesses of Drosophila melanogaster males. A pilot demonstration of experimental fitness estimation utilizing pedigree data from D. melanogaster was described. Fitnesses were obtained for the male segregants of the two "complex loci," spineless-spineless aristapedia and radius incompletus-inturned. A good fit between selection model and data was obtained, perhaps because the experimental design largely eliminated concerns of frequency or density-dependent selection and assortative mating. The selection model assumed that net fitness was divided into two components: an "early" component operative from conception to the time of observation in the generation interval, and a late component operative from the time of observation to the time of gamete pool formation. These fitness components were roughly descriptive of the physiological components viability and reproductive ability. No simple systematic relationship between the fitness of two-locus genotypes and the marginal one-locus fitness emerged. These conclusions were based on relatively precise (by current standards) fitness estimates. It was argued that pedigree data is highly desirable for fitness estimation.


The effect of phenytoin on the absorption of synthetic folic acid polyglutamate. The absorption of synthetic pteroyltriglutamate has been measured in nine normal students with and without the anticonvulsant drug phenytoin. It has been shown that phenytoin has no effect on the absorption of this folate polyglutamate. The reasons are discussed for the disparity between this result and those reported in the literature when folate polyglutamates derived from yeast were used.


The metabolism of cobalt by the normal human male: whole body retention and radiation dosimetry.


Intraluminal diverticulum of the duodenum and choledochocele. An example of intraluminal diverticulum and another of choledochocele are reported. They both presented as abdominal emergencies in adult life, and the conditions are readily shown on barium studies.


The use of higher degree statistics to estimate the number of loci which contribute to a quantitative character.


The pattern of evolutionary change in bacteria.


Littre's hernia.

Littre's hernia.


Agglutination of labeled RBC.

Agglutination of labeled RBC.


A critical review of 83 penetrating abdominal visceral injuries.


Rubella encephalomyelitis.

Rubella encephalomyelitis.


Drug abuse in the Armed Forces: an indicator of suitability, fitness or motivation for duty.


Idiopathic mesenteric venous thrombosis: report of two cases.


Death due to pulmonary air embolism in bronchial asthma: case report.


Meningioma en plaque with hyperostosis: case report.


The spectrum of infections in patients with acute leukemia and malignant lymphoma in a military hospital.


The health professionals' image.

The health professionals' image.


Radial nerve entrapment in exuberant callus from fractured humerus: case report.


Food for thought.

Food for thought.


Endocrine adaptation to malnutrition.

Endocrine adaptation to malnutrition.


Hyperphenylalaninemia and pregnancy.

Hyperphenylalaninemia and pregnancy.


Conversion of vitamin B 6  compounds in human red blood cells.


Group operant behavior in free-feeding squirrel monkeys.


The effect of disodium cromoglycate on airway conductance and on serum cortisol and serum reagins in asthma.


Treatment of aplastic anaemia with methenolone.


Cell proliferation in the "preleukaemic" phase of acute leukaemia. A cytophotometric and autoradiographic study.


The iso-electric fractionation of rabbit ferritin.


Developing applications of prostaglandins in obstetrics and gynecology. Clinical research has shown that prostaglandins (PGs) play an important role in every phase of human reproduction.  The logical consequence of early basic biological research into PGs is the current interest in clinical evaluation of PGs as agents to induce labor and abortion and to stimulate menstruation.  This is a review of the current literature dealing with PG research.  Both PGF2alpha and PGE2 are known to induce labor at term when administered by intravenous infusion.  Their relative effectiveness as compared to oxytocin has yet to be assessed.  Intravenous infusion of PGE1, PGE2, and PGF2alpha will also induce abortion.  Current research is being directed toward finding more practical methods of administration than continuous intravenous infusion.  There is hope that PGs will be able to be used as a once a month contraceptive administered at the time of expected menstruation following exposure and a possible unwanted pregnancy.  The exact mechanism of action of PGs on smooth muscle is still largely unknown.  The differences in response of a specific muscle to different PGs have also not received much research attention.  Little is known, for example, regarding the action of PGs on the cervix as opposed to the rest of the uterus.  Particularly unknown is the mechanism of action of PGs at the molecular level.  In most of the situations studied, PGs seem to stimulate adenyl cyclase activity and cyclic adenosine monophosphate formation or accumulation.


The effect of protein deprivation on pancreatic function in young animals and on animals in utero.


Involution of placental site and corpus luteum in the monkey.


Angiography in "idiopathic" focal epilepsy.


The use of trimethoprim-sulphamethoxazole in the treatment of complicated urinary tract infection.


Serum IgE in clinical immunology and allergy.


Fatty acid synthetase of chicken liver. Reversible dissociation into two nonidentical subcomplexes of similar size.


Metabolic pathways in Tetrahymena. II. Compartmentalization of acetyl coenzyme A and structure of the glycolytic and gluconeogenic pathways.


Complexes of prothrombin with calcium ions and phospholipids.


Isolation and characterization of a bacteriochlorophyll-containing protein from Rhodopseudomonas spheroides.


Enzymatic regulation of the metabolism of phosphoenolpyruvate in Tetrahymena pyriformis.


Kinetic studies with transglutaminases. The human blood enzymes (activated coagulation factor 13 and the guinea pig hair follicle enzyme.


Studies on the neutral deoxyribonuclease activity in highly purified acid deoxyribonuclease.


Studies on the site of mediated transport of chloride from blood into cerebrospinal fluid: effects of acetazolamide.


Screening for gonorrhoea in pregnancy.

Screening for gonorrhoea in pregnancy.


The role of calcium ions in the interaction of amiloride with membrane receptors.


Ultraviolet inactivation and excision-repair in Bacillus subtilis. II. Differential inactivation and differential repair of transforming markers.


Ultraviolet inactivation and excision-repair in Bacillus subtilis. I. Construction and characterization of a transformable eightfold auxotrophic strain and two ultraviolet-sensitive derivatives.


X-ray-induced loss of the maternal second chromosome in inseminated eggs of Drosophila melanogaster.


Genetic control of mutation induction in Drosophila melanogaster. I. Sex-chromosome loss in x-rayed mature sperm.


Sex-influenced reversion of the mutationally unstable mutant forked-3n of Drosophila melanogaster.


Divalent cations and the blood platelet release reaction.


Reduced lymphocyte transformation in protein calorie malnutrition.


Nociceptive response to prostaglandins and analgesic actions of aspirin and morphine.


Structural and functional effects of trabeculectomy in cynomolgus monkeys. I. Light microscopy.


Electron Microscopic study of the trabecular meshwork in alphachymotrypsin glaucoma.


Management of external fistulas of the gastrointestinal tract.


Periurethral abscess in patients with major burns.


Treatment of gallbladder rupture in an infant.


Gentamicin therapy in renal failure: a nomogram for dosage.


Heavy metal poisoning: mercury and lead.


The physiology of human penile erection.


Self-inflicted strokes.

Self-inflicted strokes.


Esters of phthalic acid as artifacts in bacterial lipids.


A study of bronchial irritation and systemic absorption of aerosolized kanamycin.


Effect of preimmunization of rabbits by means of allogenic organ antigens on survival of skin grafts.


Efficacy of nystatin topical cream in the management of cutaneous candidiasis in infants.


Anorexigenic activity of intermittent dextroamphetamine with and without meprobamate.


Evaluation of a combined muscle relaxant-analgesic as an effective therapy for painful skeletal muscle spasm.


Antiemetic effect of benzquinamide in postoperative vomiting.


The place of doxepin among the anxiolytic-sedative drugs.


The place of thiothixene among the thioxanthenes.


GP-45795: a controlled evaluation in chronic schizophrenic patients.


The effects of low-dose combinations of D-amphetamine and cocaine on experimentally induced conflict in the rat.


Characterization of pulse-labeled nuclear RNA in sea urchin embryos.


A survey of rhizobia in farm soils at Wye College, Kent.


Trace elements for growth and bulbiformin production by Bacillus subtilis.


Variation of aflatoxin content of cultures of aspergillus flavus with duration of incubation and its relation to studies on aflatoxin production.


Suppression of idiotypic specificities in adult mice by administration of antiidiotypic antibody. It has previously been shown that there are extensive idiotypic cross-reactions among antiphenylarsonate antibodies of A/J mice. The present work indicates that administration, into normal, adult A/J mice, of rabbit antiidiotypic antibody directed to A/J antiphenylarsonate antibody suppresses almost completely the subsequent production of antibody of the corresponding idiotype. No effect was noted on the formation of antibodies to the protein carrier or of antiphenylarsonate antibody of a different idiotype. The data are consistent with central suppression of production of the idiotypic antibody mediated through interaction with immunoglobulin receptors on lymphocytes.


Immunosuppression of mice injected with heterologous anti-immunoglobulin heavy chain antisera. Neonatal injection of mice with rabbit anti-micro antiserum has been shown to produce complete loss of direct and indirect plaque-forming responses to sheep erythrocytes as well as loss of serum IgM and severe depressions of all other serum immunoglobulins. Similar injection of anti-gamma1gamma2 or anti-gamma1 antibodies effects a loss of the indirect response but induces relatively minor alterations in serum Ig levels. Delaying initiation of anti-micro treatment until young adulthood results in a somewhat diminished effect on plaque-forming responses and serum Ig levels but triggers the release of high serum levels of an aberrant micro-bearing protein. Anti-micro suppression of genetically thymusless mice indicates that at least part of the target cells for suppression are bone marrow derived. A working hypothesis for the maturation of humoral antibody-producing cell lines as it relates to these data is discussed.


Genetic and metabolic bases of two "albino" phenotypes in the leopard frog, Rana pipiens.


Separation of stages in the development of the "T" cells involved in cell-mediated immunity. Density distribution analysis in continuous gradients of albumin has been used to study the development of cytotoxic lymphocytes (CL), to separate and characterize the progenitors of CL, and to determine their relationship to subpopulations of T cells. CL progenitors in the thymus were a homogeneous, medium-density population, distinct from the typical, dense, thymus small-lymphocyte. Activity seemed to be associated with one minor subpopulation of cells with surface antigenic properties characteristic of peripheral T cells (high levels of H-2 antigen, low levels of theta-antigen). CL progenitors in the spleen differed from those in the thymus and normally had the high buoyant density of typical small T lymphocytes. In states of antigenic stimulation, some lighter-density CL progenitors were found in the spleen. The buoyant density of the CL population developing in the spleens of immunized animals showed progressive changes with time. Early, "immature" CL had the light-density characteristics of large, activated lymphocytes. As the response developed, the density of the CL population increased, and finally approached that of CL progenitors and of typical small lymphocytes. The data suggest that density subpopulations of T cells represent stages in the development of immunocompetent cells. Possible differentiation pathways of T lymphocytes in the thymus and in the spleen are discussed.


Assay of prolactin in vertebrate pituitaries by its dispersion of xanthophore pigment in the teleost Gillichthys mirabilis.


Effect of interruption of the enterohepatic circulation of bile on the secretion of iodipamide in the bile of the rhesus monkey.


A purified procoagulant enzyme from the venom of the eastern diamondback rattlesnake (Crotalus adamanteus): in vivo and in vitro studies.


Determination of body composition by neutron activation analysis in patient with renal failure.


Unrelatedness of temperate Bacillus subtilis bacteriophages SP02 and phi105. SPO2 and phi105 are temperate Bacillus subtilis bacteriophages which have been suggested to belong to a cluster of related bacteriophages. In the present work, we show that SPO2 does not complement any of the 11 essential genes known in phi105 and that the phages do not recombine. Deoxyribonucleic acid (DNA)-DNA hybridization shows less than 10% homology between SPO2 and phi105 DNA. DNA synthesis in phi105 shows a greater dependence on host functions than does SPO2 DNA synthesis. Growth of phi105 but not of SPO2 is inhibited by the uracil analogue 6-(p-hydroxyphenylazo)-uracil. Infection of a DNA polymerase-deficient strain of B. subtilis with SPO2 leads to an increase in DNA polymerase activity in crude extracts, whereas no such increase is found after infection of this strain with phi105. It is concluded that SPO2 and phi105 are unrelated bacteriophages.


Nucleic acid synthesis in bacteriophage SPO2c 1 -infected Bacillus subtilis. The synthesis of host macromolecules was shut off very slowly and incompletely by bacteriophage SPO2c(1). No change in the rate of incorporation of radioactive precursors into protein and ribonucleic acid (RNA) could be detected after infection, and the rate of incorporation of thymidine was increased only slightly. The relative proportions of phage and host species of nucleic acids at various intervals in the latent period were determined by means of nucleic acid hybridization. Phage-specific RNA populations synthesized early were different from those synthesized late in the latent period. Host deoxyribonucleic acid (DNA) replication continued until 8 to 10 min after SPO2c(1) infection and then decreased markedly as phage-specific DNA synthesis was initiated. Host DNA was not degraded to trichloroacetic acid-soluble fragments, and its nucleotides were not found in either newly synthesized intracellular phage DNA or in progeny phage particles. The average burst size of SPO2c(1) was approximately 200 plaque-forming units per cell.


Nonparenteral serum hepatitis. Report of an outbreak.


Experimental infection with Plasmodium falciparum in Aotus monkeys. I. Parasitologic, hematologic, and serum biochemical determinations.


Experimental infection with Plasmodium falciparum in Aotus monkeys. 3. The development of disseminated intravascular coagulation.


The high cost of health.

The high cost of health.


Characterization of a guanylic acid specific ribonuclease from Aspergillus fumigatus.


A phospholipase in Bacillus megaterium unique to spores and sporangia.


Effect of calcium ion on the structure of native bovine casein micelles.


Alterations in mechanoreceptor input to Brodmann's areas 1 and 3 of the postcentral hand area of Macaca mulatta after nerve section and regeneration.


Thalamo-caudate-cortical relationships in synchronized activity. II. Further differentiation between spindle systems by cooling and lesions in the mesencephalon.


Subendocardial distribution of coronary blood flow and the effect of antianginal drugs.


Focal transient cerebral ischemia in the squirrel monkey. Effect on brain adenosine triphosphate and lactate levels with electrocorticographic and pathologic correlation.


The location of the chemoreceptor controlling growth hormone secretion during hypoglycemia in primates.


Urinary estrogens and estrogen metabolism of the rhesus monkey.


Glutaraldehyde-fixation method for determining the permeability to water of the toad urinary bladder.


The infleunce of fetal sex on plasma progesterone levels.


Assimilation and contrast effects in visual discrimination by rhesus monkeys.


Characterization of growth hormone of different molecular weights in rat, dog and human pituitaries.


The conversion of acetate to cholesterol in the fetus of the baboon and the transfer of cholesterol from mother to fetus.


The polypeptides of adenovirus-infected cells.


Evidence for a new type-specific structural antigen of the influenza virus particle.


VA-community nursing home care program called outstanding success.


Nursing home costs are rising--and so is government share.


Fluphenazine-induced parkinsonism in the baboon: pharmacological and metabolic studies.


Levels and distribution of central nervous system amines in normal and morphine-dependent monkeys.


Molecular basis for the attack on cancer. Studies of cell transformation by RNA and DNA tumor viruses by DNA-RNA hybridization and extension of these techniques to analysis of human cancer tissue are reviewed. The molecular hybridization technique, it is suggested, will be useful in further studies of the role of oncogenic RNA and/or DNA viruses in causation of cancer.


Electron transfer to ferricytochrome c: reaction with hydrated electrons and conformational transitions involved. The reaction of horse-heart cytochrome c with hydrated electrons has been studied by the pulseradiolysis technique. In neutral solution, the ferriheme group was reduced in a bimolecular reaction that takes place at a rate equal to that of the decay of the e(-) (aq), and approaches the diffusion-controlled limit. This reduction is assigned mainly to a direct reaction, proceeding via the exposed edge of the porphyrin projecting into the cytochrome c crevice. The reaction absorption spectrum observed 20 musec after an electron pulse was very similar, yet blue-shifted relative to, the difference spectrum between the reduced and oxidized forms of cytochrome c. However, this shift vanishes in a slow monomolecular reaction, which seems to reflect the conformational relaxation of the protein to the final equilibrium state of its reduced form. In alkaline solutions, the transition of cytochrome c molecules into an irreducible conformation causes a proportionate decrease in the amount of ferricytochrome c reduced in the direct reaction. The rate of conformational transition of the protein into the reactive form is now the limiting step for a substantial part of the reduction that takes place via this slow monomolecular reaction. Pseudomonas cytochrome c 551 which, in contrast to horse-heart cytochrome c, is a negatively charged protein at neutral pH reacts with e(-) (aq) at a rate lower than does the horse-heart protein. The reduction of the heme group follows that of the e(-) (aq) decay with a small, yet significant, delay.


Regulation of malanin synthesis in mammalian cells: effect of gene dosage on the expression of differentiation. Near-tetraploid (2S), pigmented, Syrian hamster cells (3460(2)) were hybridized with unpigmented mouse fibroblasts to produce hybrids that contained two pigment cell genomes and one fibroblast genome per cell (3(2)D). Half of the hybrids were pigmented and showed high activity of the enzyme dihydroxyphenylalanine oxidase; the other half of the hybrids were unpigmented and devoid of the activity of this enzyme. (In contrast, all the previously isolated hybrids containing only one pigment cell genome and one fibroblast genome were unpigmented.) Analyses of the chromosome complement and of a series of enzymes unrelated to melanin synthesis did not reveal any great differences between the pigmented and unpigmented 3(2)D hybrids. Upon the isolation of subclones, unpigmented 3(2)D hybrids produced only unpigmented colonies, whereas pigmented hybrids produced both pigmented and unpigmented colonies. Although the mechanism that results in the formation of some pigmented and some unpigmented hybrids in these experiments is not known, it is clear that the number of pigment cell genomes in a hybrid affects the expression of pigmentation.


Role of the physician in medical cost control.


Incontinentia pigmenti (Bloch-Sulzberger syndrome).


Fetal monitoring.

Fetal monitoring.


Neurofibromatosis in a family.

Neurofibromatosis in a family.


Brain and eye abnormalities. Possible sequelae to prenatal use of multiple drugs including LSD.


Do Rh-negative women with an early spontaneous abortion need Rh immune prophylaxis?


Characteristics of the nitrogenase system of the blue-green alga Anabaena cylindrica.


Effect of degradation of rat-liver ribosomal RNA on the structure of the ribosomal subunits.


Study on the mechanism of lysosome labilization by inorganic mercury in vitro.


RNA synthesis in Tetrahymena. Temperature-pressure studies.


Studies on the division cycle of mammalian cells. VI. DNA polymerase activities in partially synchronous suspension cultures.


An ergot alkaloid preparation (hydergine) in geriatric therapy.


Obesity following combination of rostrolateral to VMH cut and contralateral mammillary area lesion.


Mother-infant separation in the Java monkey (Macaca irus).


G-6-PD hemolytic anemia complicating diabetic ketoacidosis.


The changing pattern of diseases associated with M components.


IgG subclasses: development of the serum concentrations in "normal" infants and children.


Interrelations of humans, dogs, and rodents.


Gonorrhea in adolescent girls in a closed population. Prevalence, diagnosis, and treatment.


Streptococcal pharyngitis therapy. Comparison of cephalexin, phenoxymethyl penicillin, and ampicillin.