The experimental production of hyaline membranes in premature rhesus monkeys. When premature rhesus monkeys are ventilated with oxygen or air, hyaline membranes are formed within 5 minutes in alveolar ducts and respiratory bronchioles. Studies with fluorescein-labeled antiserum to porcine pepsinogen provide evidence that the monkey fetuses have aspirated gastric fluid. The findings suggest that some component of gastric fluid plays an important role in the formation of the hyaline membranes, either directly by causing necrosis of susceptible bronchiolar epithelium or secondarily by sensitizing the bronchiolar epithelium to another necrotizing agent such as oxygen.


Platelet interaction with bacteria. 3. Ultrastructure. The interaction of several common strains of bacteria with rabbit or human platelets in vitro has been examined sequentially with scanning and transmission electron microscopy. Bacteria were added to platelets in their native plasma or to washed platelets in a balanced salt solution at ratios of about 1:1 or at low bacteria to platelet ratios (down to 1:100). The platelet-bacterial interaction (PBI) was studied with recording nephelometry. Matched samples were fixed for microscopy at various points in the aggregation response. The results support these conclusions: a) Bacteria stimulate platelet aggregation by direct contact and adhesion with the platelet surface. b) Adhesion between the two cell types requires divalent cations, occurs through fusion of normal cell-surface coats and appears identical in the presence or absence of extracellular plasma protein. c) The morphologic transformation of platelets during PBI is identical to that produced by collagen. d) During PBI the bacteria are incorporated into the forming platelet aggregates and reside predominantly intercellularly. e) Phagocytosis of bacteria by a single platelet is very rare. f) Bacteria which have resided within platelet aggregates for one hour are unaltered morphologically. g) PBI occurs even at very low bacterial numbers and produces platelet-bacterial aggregates in small numbers without stimulating generalized platelet aggregation. Methods for concentration of thrombocytopenic plasma and washing human platelets are presented.


Animal model: pulmonary hypertension, cor pulmonale and endocardial fibroelastosis in monocrotaline-intoxicated nonhuman primates.


Experimental studies of hyperaemic phenomena in the cerebral circulation of primates.


The role of the massa intermedia in motor performance in the Rhesus monkey.


Our personal clinical experience in total parenteral feeding.


Autoregulation of the secretion of MSH in incubates of toad pars intermedia.


Effects of hypothalamic extirpations on thyroidal and intermedinal activity in the red spotted newt.


RNA fractionation on modified celluloses. I. Ecteola-, ectham-, amino-ethyl-, nucleic acid-, and nitro-cellulose.


RNA fractionation on modified celluloses. II. DEAE-cellulose.


Mycoplasmal flora of simians.

Mycoplasmal flora of simians.


Serologic heterogeneity of the mycoplasmatales.


Antigenicity of Mycoplasmatales.

Antigenicity of Mycoplasmatales.


Pathogenesis of Ross River virus infection in mice. II. Muscle, heart, and brown fat lesions.


Pathogenesis of Ross River virus infection in mice. I. Ependymal infection, cortical thinning, and hydrocephalus.


Phenol-water fractions from smooth Brucella abortus and Brucella melitensis: immunochemical analysis and biologic behavior.


Classification of Neisseria meningitidis group B into distinct serotypes. 3. Application of a new bactericidal-inhibition technique to distribution of serotypes among cases and carriers.


Aggravating and protecting effects of triton and corticosteroids in galactosamine-induced hepatitis.


Isolation and purification of hepatitis-associated antigen by affinity chromatography with baboon antiserum.


Serologic aspects of experimental histoplasmosis in cattle.


Physical evidence of a plasmid in Neisseria gonorrhoeae.


Epidemic diphtheria and skin infections in Trinidad.


New method for study of infections with Pneumocystis carinii.


Is the proper study of mankind man?

Is the proper study of mankind man?


Pulmonary mycetoma and its surgical management.


Genetic properties of arsenate sensitive mutants of Bacillus subtilis 168.


Transformation and transduction of a large deletion mutation in Bacillus subtilis.


DNA replication during development of competence in Bacillus subtilis.


Progress in stereotaxic surgery.

Progress in stereotaxic surgery.


Myoclonic epilepsy treated by combined stereotactic lesions (a case report).


Phase three--unsound, unfair.

Phase three--unsound, unfair.


Travase, an effective enzyme for burn debridement.


Intrathoracic transposition of the transverse colon in complicated oesophageal atresia.


The in vitro biosynthesis and metabolism of progesterone and estrogens by chimpanzee placental tissue.


Postreinforcement changes of steady potentials in premotor cortex of monkeys.


Metabolic emergencies.

Metabolic emergencies.


Triglyceride clearance during diets rich in carbohydrate or fats.


Behavior of infant in causation of nutritional marasmus.


Pancreas in primary malnutrition disorders.


Hormonal control of endometrial glycogen metabolism in the Macaca arctoides.


Progesterone values in monkeys near term.


Sexual behavior during successive ejaculations in bonnet and pigtail macaques.


Seasonal variation in sexual behavior of all-male rhesus troops.


Hormonal and social factors affecting evoked sexual behavior in Rhesus monkeys.


Copulatory behavior in relation to anatomical characteristics of three macaques.


Prenatal dental development in the black howler monkey (Alouatta caraya).


Germ cell chimerism in male marmosets.

Germ cell chimerism in male marmosets.


Karyotypes of squirrel monkeys (Saimiri sciureus) from different geographic regions.


Biologic basis for maxillary osteotomies.


Morphogenetic experiments in facial asymmetry: the nasal cavity.


Experimental radio-osteonecrosis in Rhesus macaque jaws; therapeutic irradiation dose effect on dental extraction wound healing.


Diurnal heart rate and body temperature in marmosets.


Gastric emptying time and absorption of simple and complex carbohydrates in Macaca mulatta.


A chronic implant for recording of cochlear potentials in primates.


Hearing of old world monkeys (Cercopithecinae).


Visual sensitivity in the squirrel monkey.


Visual-optical characteristics of caged and semifree-ranging monkeys.


Evolution of the primate visual system: anterograde degeneration studies of the tecto-pulvinar system.


Effect of optic chiasmotomy upon serotonin concentration in the pineal gland of the monkey.


Maze behavior in macaques.

Maze behavior in macaques.


An experimental study of vocal communication between mother and infant monkeys (Macaca nemestrina).


Cardiac allograft pathology in Rhesus monkeys.


Analysis of Rhesus monkey vocalizations: maturation-related sex differences in clear-cell frequency.


Acoustical communication among free-ranging common indian langurs (Presbytis entellus) in two different habitats of North India.


Tactile communication among free-ranging langurs.


Herpesvirus saimiri antigens and virus recovery from cultured cells and antibody levels and virus isolations from squirrel monkeys.


Isolation and characterization of a new virus from owl monkeys: herpesvirus aotus type 3.


Control of losses in freshly-imported laboratory primates during the acclimatization period.


Visual and tactile communication in Macaca arctoides and its ontogenetic development.


Labor and delivery behavior in rhesus monkeys (Macaca mulatta).


The social behavior of adult male-infant pairs of Rhesus macaques in a laboratory environment.


Experimental analysis of causal relationships between grooming behavior and inter-individual proximity in the stumptailed macaque (Macaca arctoides): a preliminary report.


The influence of target sex and dominance on evoked attack in Rhesus monkeys.


An ethogram for Rhesus monkeys. I. Antithetical contrasts in posture and movement.


Maternal and paternal periods in the development of infant Callimico goeldii.


Handedness in the common marmoset (Callithrix jacchus).


On the function of facial muscles in different behavioral situations. A study based on muscle morphology and electromyography.


Memory in monkeys as a function of preparatory interval and pattern complexity of matrix displays.


Observation learning of tool use by captive Guinea baboons (Papio papio).


Social responses to abnormal infant monkeys.


Social organization of wild talapoin monkeys.


Outcast males and social structure among bonnet macaques (Macaca radiata).


Familial interactions of male rhesus monkeys in a semi-free-ranging troop.


Night observations of free-ranging Rhesus monkeys.


The timing of fission among free-ranging Rhesus monkeys.


Pilot experiments to test group tolerance to a stranger in wild Macaca fascicularis.


Observations on two types of neotropical primate intertaxa associations.


Synecology of Bornean primates. I. A test for interspecific interactions in spatial distribution of five species.


Brain theory and minimal brain dysfunction.


Control of the general amino acid permease of Penicillium chrysogenum by transinhibition and turnover.


Intravenous hyperalimentation.

Intravenous hyperalimentation.


A comparison of microfilariae isolated from canine blood by the modified Knott test and a filter method.


Studies on the structure of metaphase and interphase chromatin of Chinese hamster cells by circular dichroism and thermal denaturation.


Comparison of serum protein-bound carbohydrate and glycoprotein patterns of man, monkey and rat.


Problems of iron-deficiency anaemia in infancy.


Proliferation and migration of reticuloendothelial cells following injection of a tubercle bacillary lipid.


Gonococcaemia in the family.

Gonococcaemia in the family.


How infectious is gonorrhoea?

How infectious is gonorrhoea?


Disappearance of diphtheria.

Disappearance of diphtheria.


Internal fixation of secondary deposits in long bones.


Cerebellar cortical degeneration due to acute azide poisoning.


Injury to the lateral ligament of the ankle: a clinical study.


Effect of anti-cord factor antibody on experimental tuberculosis in mice. Either active immunization with trehalose-6, 6'-dimycolate (cord factor)-methylated bovine serum albumin complex or passive transfer of rabbit anti-cord factor serum induced in mice an enhanced resistance against infection with virulent human Mycobacterium tuberculosis. This suggests that the anti-cord factor antibody exerts an infection-protecting effect by neutralizing the toxic action of cord factor during the course of living virulent infection with tubercle bacilli. The protective effect of active immunization with cord factor-methylated bovine serum albumin complex and of anti-cord factor serum was found to be specific for the tuberculous infection among a number of experimental infections in mice with a variety of cytopathogenic microorganisms tested.


Protection of mice against vaccinia virus by bacterial infection and sustained stimulation with specific bacterial antigens. In these experiments, mice which have a strong delayed-type hypersensitivity to mycobacteria were found, when elicited with old tuberculin, to be more resistant to intravenous vaccinia virus challenge than controls. This was manifest as protection from killing when large amounts of virus were injected, or as significantly less tail swelling and damage as well as lower titers of infectious virus when a lesser inoculum was used. Preliminary experiments indicate that animals sensitized with Staphylococcus aureus and elicited with phage lysate of staphylococcus are also more resistant to vaccinia infection.


Effect of hypersensitivity pneumonitis on the pulmonary defense mechanisms of guinea pig lungs. Many edemagenic and consolidating inflammatory diseases, such as virus pneumonias, of the lung are complicated by bacterial infection. Previous literature has stressed that edema and consolidation may promote bacterial proliferation by interfering with phagocytosis. To test that hypothesis, lung defense mechanisms were studied in guinea pigs with tuberculin-induced hypersensitivity pneumonitis, a noninfectious edemagenic, and consolidating inflammatory disease. Pulmonary bactericidal activity and particle clearance were measured with a mixed aerosol of (32)P-labeled Staphylococcus aureus and(35)S-labeled Proteus mirabilis. Hypersensitivity pneumonitis enhanced the bactericidal activity of the lung but had no effect on particle clearance despite the presence of consolidation and edema. These data indicate that altered host resistance to bacterial infection in acute inflammatory lung diseases can not be attributed to edema, inflammation, consolidation, changes in lung weight, etc., per se and that causes must be sought in functional changes in the bactericidal system of the lung rather than in specific histopathological changes.


Rickettsial hemolysis: adsorption of rickettsiae to erythrocytes. The hemolysis of sheep red blood cells by Rickettsia prowazeki has been characterized. A requirement for the adsorption of the rickettsia to the erythrocyte was indicated. No indication of the production of a soluble hemolysin was observed. Neither adsorption nor hemolysis can proceed at 0 C. The hemolytic process is rapid with a half-time of less than 30 min, and both the rate and extent are proportional to the number of rickettsiae present.


A study of the O and protein antigens of Salmonella adelaide and Escherichia coli 0111:B4 by agar diffusion techniques.


A study of meningococci isolated from patients and carriers in Israel during 1966-71.


Effect on EDTA on human cumulus granulosa cells.


The modification, by the addition of carbonic anhydrase, of the effect of non-gaseous acids on the carotid body chemoreceptor discharge of the cat.


Cell multiplication in Tetrahymena cultures after addition of particulate material.


Administration of melatonin to non-human primates and to women with breast carcinoma.


The nature of the host in the origin of the eukaryote cell.


Participation of epileptic patients in sports.


Acute leukemia following cytotoxic chemotherapy.


Adult coeliac disease and multiple small bowel diverticula.


Intravenous hyperalimentation without sepsis.


Enhancement of endotoxin shock in the lead-sensitized subhuman primate.


A silicone rubber atrial catheter for prolonged parenteral alimentation.


A new subcutaneous arteriovenous shunt: applicable in cases where the standard Quinton-Scribner shunt and arteriovenous fistula have failed.


Technique of insertion of central venous catheter for parenteral hyperalimentation in infants.


Presence and origin of human IgG subclass proteins in newborns.


Anti-Fy5, and antibody disclosing a probable association between the Rhesus and Duffy blood group genes.


Multiple jejunal diverticulosis and small bowel volvulus.


The diagnosis of brucellosis in dairy herds.


Listeria as a cause of abortion and neonatal mortality in sheep.


Halobacterium cutirubrum ribosomes. Properties of the ribosomal proteins and ribonucleic acid. 1. The 30S ribosomal subunit of the extreme halophile Halobacterium cutirubrum is unstable and loses 75% of its ribosomal protein when the 70S ribosome is dissociated into the two subunits. A stable 30S subunit is obtained if the dissociation of the 70S particle is carried out in the presence of the soluble fraction. 2. A fractionation procedure was developed for the selective removal of groups of proteins from the 30S and 50S subunits. When the ribosomes, which are stable in 4m-K(+) and 0.1m-Mg(2+), were extracted with low-ionic-strength buffer 75-80% of the 30S proteins and 60-65% of the 50S proteins as well as the 5S rRNA were released. The proteins in this fraction are the most acidic of the H. cutirubrum ribosomal proteins. Further extraction with Li(+)-EDTA releases additional protein, leaving a core particle containing either 16S rRNA or 23S rRNA and about 5% of the total ribosomal protein. The amino acid composition, mobility on polyacrylamide gels at pH4.5 and 8.7, and the molecular-weight distribution of the various protein fractions were determined. 3. The s values of the rRNA are 5S, 16S and 23S. The C+G contents of the 16S and 23S rRNA were 56.1 and 58.8% respectively and these are higher than C+G contents of the corresponding Escherichia coli rRNA (53.8 and 54.1%).


Thermal inactivation and reactivation of an enzyme in vivo. Pantothenate hydrolase of Pseudomonas fluorescens. Thermal inactivation and reactivation of pantothenate hydrolase were studied in whole cells of Pseudomonas fluorescens. The enzyme is susceptible to thermal inactivation in whole cells at 37-40 degrees C, and is reactivated when the temperature is lowered again. Chloramphenicol does not prevent reactivation. The activation energy of enzyme inactivation in vivo is about 540kJ/mol. This activation energy is 220kJ/mol in vitro, but it is increased to 550-630kJ/mol by several metabolites, such as succinate, glyoxylate and oxalate. Generally, good carbon sources, causing rapid growth, protect the enzyme from thermal inactivation in vivo, and enable reactivation to occur at a fast rate. The enzyme is also inactivated below 35 degrees C, showing an activation energy of about 35kJ/mol. Good carbon sources prevent this inactivation as well, and cause slight reactivation. Glycine, although not utilized for growth, protects the enzyme well from this inactivation but not from inactivation at 37-40 degrees C, and prevents reactivation totally. From the activation energies of inactivation and the effects of the various carbon sources, it appears possible that changes in the concentrations of intracellular metabolites may be responsible for the changes in inactivation and reactivation.


Activation of liver glycogen synthetase in vitro.


The role of zinc ions in the transformation of lymphocytes by phytohaemagglutinin. 1. Incorporation of [(3)H]thymidine into DNA was inhibited by EDTA and diethylenetriamine-NNN'N''N''-penta-acetate but not by nitrilotriacetate even when Ca(2+) was present at more than twice the concentration of the chelators. 2. The inhibition caused by EDTA was most effectively reversed by Zn(2+) but also to a lesser extent by Cd(2+). Very little if any activation of the EDTA-inhibited system was obtained with Co(2+), Cu(2+), Fe(3+), Mn(2+) or Ni(2+) added alone. 3. Fe(3+) added to the Zn(2+)-activated lymphocytes in the presence of EDTA markedly increased thymidine incorporation. Addition of Cd(2+) prevented the inhibition of incorporation which occurred at high Zn(2+) concentrations. 4. If EDTA was added more than 15h after phytohaemagglutinin, the inhibition of incorporation was less than that obtained by its addition at zero time. If Zn(2+) was added later than 12h after EDTA and phytohaemagglutinin, the inhibition of incorporation by EDTA was not fully reversed. A study of the time-course of the effects of delayed additions of EDTA and Zn(2+) suggested that, on average, the cells required Zn(2+) between 20 and 30h after phytohaemagglutinin addition to allow the full rate of thymidine incorporation at 37h. 5. The increase in the rate of protein synthesis caused by phytohaemagglutinin was not inhibited by EDTA until about 8h. The further increase after this was totally inhibited by EDTA but this inhibition was fully reversible with Zn(2+). The rate of protein synthesis in EDTA-inhibited cultures at 40h was the same as that at 10h. 6. There was a close similarity between the effects of EDTA on lymphocyte stimulation and those reported by Kay et al. (1969) with low doses of actinomycin D.


Metabolic regulation in the C4 dicarboxylic acid pathway of photosynthesis.


Studies on implanted islets of Langerhans: normalization of blood glucose concentration, blood insulin concentration and weight gain in New Zealand obese mice.


Polarity in the gua operon of Escherichia coli K 12.


Low-molecular-weight solutes released during mild acid hydrolysis of the lipopolysaccharide of Pseudomonas aeruginosa. A careful examination of the low-molecular-weight solutes released during mild acid hydrolysis of the lipopolysaccharide of Pseudomonas aeruginosa (N.C.T.C. 1999) revealed the presence of ethanolamine triphosphate. During storage, the compound decomposed to give ethanolamine pyrophosphate, identified in a previous study (Drewry et al., 1971); PP(i) may be a further decomposition product. Evidence for the attachment of ethanolamine triphosphate to a polysaccharide fraction was obtained, but the possibility that some was attached to the lipid A moiety was not excluded. Basic compounds released during the hydrolysis of lipopolysaccharide included amino acids, polyamines and oligopeptides.


The structure and regulation of the lactose synthetase system.


A proposed structure for Streptomyces griseus protease A.


The acylation of endogenous lysophospholipids in isolated rat liver microsomal fraction: influence of glutathione and ethylenediamine-tetra-acetate on this process.


Intra-pelvic pressure and renal function studies in experimental chronic partial ureteric obstruction.


Local nasal desensitization in allergic rhinitis.


Observations on the safety of disodium cromoglycate in long-term use in children.


Exercise induced asthma: response to disodium cromoglycate in skin-test positive and skin-test negative subjects.


Precipitins to Micropolyspora faeni in the adult cattle of selected herds in Scotland and north-west England.


Asthma due to inhaled chemical agents--piperazine dihydrochloride.


Asthma due to inhaled chemical fumes--amino-ethyl ethanolamine in aluminium soldering flux.


Differential density labeling and gradient centrifugation of Tetrahymena: a new procedure for selection synchrony.


Clinical trial of topical disodium cromoglycate in vernal kerato-conjunctivitis.


Localization of DNA in ultrathin tissue sections incubated with terminal deoxynucleotidyl transferase, as visualized by electronmicroscope autoradiography.


Aging redefined, or the ravages of time debunked.


Causes of increase in Medicaid costs in California.


Radioimmunoassay of HGH, ICSH and FSH in plasma after i.v. addministration of thyrotropin releasing hormone (TRH).


Different patterns of thyrotropin (TSH)-release, TSH-resynthesis and of corticosterone depression after in vivo administration of thyrotropin-releasing hormone (TRH) and of an isosteric analog of TRH.


The distribution and elimination of  125 I-labelled thyrotropin-releasing hormone in the mouse.


Repetitive peer separation of young monkeys: effects of vertical chamber confinement during separations.


Inhibition of thyrotropin releasing hormone (TRH) stimulated thyrotropin (TSH) secretion in man by a single oral dose of thyroid hormone.


Osteomalacia in neurofibromatosis.

Osteomalacia in neurofibromatosis.


-L-rhamnosidases of the liver of Turbo cornutus and Aspergillus niger.


Comparison of properties and inducibility of glutamate dehydrogenases in rat kidney and liver.


Isolation and some properties of glyceraldehyde 3-phosphate dehydrogenase from vegetative cells of Bacillus cereus.


Drosophila melanogaster: effects of gamma radiation on fecundity and longevity.


In vitro attempts to infect primate cells with the nucleopolyhedrosis virus of Heliothis.


Bidirectional chromosome replication in Bacillus subtilis.


Induction of virus-inhibiting factor or interferon in mice by strains with different virulence of Rift Valley fever virus.


Hexachlorophene.

Hexachlorophene.


Infant antiseptic skin care and hexachlorophene.


Endogenous amino acid loss and its significance in infantile diarrhea.


Ultramicrochemical assay of periodontal ligament obtained by apoxesis.


Effect of whole-body vibration on peripheral nerve conduction time in the rhesus monkey.


Behavioral changes from chronic exposure to pesticides used in aerial application: effects of phosdrin on the performance of monkeys and pigeons on variable interval reinforcement schedules.


Retinal damage thresholds for multiple pulse lasers.


Protection of monkeys against airborne tuberculosis by aerosol vaccination with bacillus Calmette-Guerin.


Immune responses in rhesus monkeys after bacillus Calmette-Guerin vaccination and aerosol challenge with Mycobacterium tuberculosis.


Microbiologic analysis of niacin from individual colonies of Mycobacterium tuberculosis as a diagnostic adjunct.


Allergic bronchial reactions to inhalation of enzymes of Bacillus subtilis.


Pseudomonas aeruginosa pneumonia in adults.


Avian tuberculosis in monkeys. A unique mycobacterial infection.


Neonatal hepatitis associated with Australia antigen (Au-1).


Hepatic glycogen synthetase deficiency.

Hepatic glycogen synthetase deficiency.


Interocular transfer in the monkey: rostral corpus callosum mediates transfer of object learning set but not of single-problem learning.


Functional interpretation of the flash-evoked response in the chiasma of the monkey (Cebus albifrons).


Immunological studies with rat prostate: antigenic localization and effect of hormones.


Cryosurgery for cancer.

Cryosurgery for cancer.


Common generalized epilepsy. The so-called idiopathic or centrencephalic epilepsy.


Immunospecific isolation of antibodies by dissociation from virus or particulate antigen adsorbed on charcoal.


Experimental autoimmune damage to rabbit male accessory glands.


The PMPO syndrome (postmenopausal palpable ovary syndrome).


Human carbonic anhydrases. X. Preparation of large peptide fragments of carbonic anhydrase B used for sequence studies.


Classics in oncology. A sarcoma involving the jaws in African children.


Humanism and cancer.

Humanism and cancer.


Human carbonic anhydrases. XI. The complete primary structure of carbonic anhydrase B.


Crystalline L-histidinol phosphate aminotransferase from Salmonella typhimurium. Purification and subunit structure.


A progress report. Detection of colorectal cancer using guaiac slides.


Unproven methods of cancer management. Zen macrobiotic diet.


Cutaneous signs of internal cancer.

Cutaneous signs of internal cancer.


Simultaneous isolation and characterization of chicken supernatant and mitochondrial isoenzymes of aspartate transaminase.


Turnover of bacterial cell wall peptidoglycans.


Mucoid variation in Pseudomonas aeruginosa induced by the action of phage.


Routine identification of group-C streptococci by means of an agglutinin (protectin) from the albumen gland of the edible snail, Helix pomatia.


The use of  -lactamases in the clinical laboratory.


Evaluation of an immunodiffusion test for the detection of antibodies to Brucella abortus in bovine serum.


Sensitivity of respiratory strains of Pseudomonas aeruginosa to carbenicillin.


Production of enzymes and toxins by hospital strains of Pseudomonas aeruginosa in relation to serotype and phage-typing pattern.


New attachment after treatment of interradicular lesions.


An improved method for the isolation of highly polymerized native deoxyribonucleic acid from certain protozoa.


Macro- and micronuclei of Tetrahymena pyriformis: a model system for studying the structure and function of eukaryotic nuclei.


Contractility and its control in peritrich ciliates.


Second roundtable discussion on taxonomic problems relating to malaria parasites.


Four new species of Eimeria and one of Tyzzeria from the Ceylon jungle fowl Gallus lafayettei.


A scanning electron microscope study on Gregarina sp. from Udeopsylla nigra.


Structure, cytochemistry, and locomotion of Haemogregarina sp. from Rana berlandieri.


Crystalloid inclusions in species of Leucocytozoon, Parahaemoproteus, and Plasmodium.


A comparative study of gametocyte ultrastructure in avian haemosporidia.


The positioning of ciliary organelles in hypotrich ciliates.


Persistent pesticides and PCBs in the environment.


Transmission of kuru from man to rhesus monkey (Macaca mulatta) 8 and one-half years after inoculation.


Effects of polychlorinated biphenyl on marine phytoplankton communities.


PCB contamination from boat bottom paint and levels of PCB in plankton outside a polluted area.


Elevated vanyl-amndelic acid excretion in severe diabetic keto-acidosis.


Shock thresholds of low- and high-protein-reared rhesus monkeys.


Experience with rubella vaccines in the U.S.A., 1969-1971.


The control of rubella vaccines.

The control of rubella vaccines.


Primary drug resistance. 3.

Primary drug resistance. 3.


Contaminated stethoscopes: a potential source of nosocomial infections.


The effect of fungal metabolites and of coriander seeds on the growth of pathogenic bacteria.


Genetic variation in the carbonic anhydrase isozymes of macaque monkeys. I. The radioimmunosorbent assay.


Genetic variation in the carbonic anhydrase isozymes of macaque monkeys. II. Inheritance of red cell carbonic anhydrase levels in different carbonic anhydrase I genotypes of the pig-tailed macaque, Macaca nemestrina.


Tuberculosis of the spine: a reminder.

Tuberculosis of the spine: a reminder.


"Shock lung" syndrome following diabetic ketoacidosis; treatment with heparin.


Isoenzymes of amino acid naphthylamidase in the synovial membrane and in synovial fluid.


Immunochemical similarities between major and minor components of human placental alkaline phosphatase.


Formation of chloroplast ribosomes and ribosomal RNA in Euglena incubated with protein inhibitors.


Structure and function of cytoplasmic organelles in transiently and permanently bleached Euglena.


RNA synthesis and acquisition of actinomycin D insensitivity during conjugation in Stylonychia mytilus.


Importance of the nucleolus in the initiation of DNA synthesis in mammalian cells. Studies with an ultraviolet microbeam and low concentrations of actinomycin D.


Mitotic spindles from Drosophila melanogaster embryos.


Teletherapy simulator: design and clinical applications in radiotherapy.


Mechanism for the regulation of cell division in Agmenellum. We describe a nonlethal temperature-conditional mutant of Agmenellum quadruplicatum which allows dissociation of the processes of growth and cell division. With this system, evidence has been obtained for the regulation of one step in the process of cell division by a small effector molecule. The effector molecule is apparently released into the surrounding medium and can be obtained from lyophilized spent medium by extraction with 80% ethanol. The addition of this extract to serpentine filaments of the SN29 mutant strain stimulates cell division in these filaments and leads to the production of cells approximating normal dimensions within one generation time. The degree of stimulation of cell division is directly related to the amount of extract added. A general hypothesis is presented for the positive regulation of the initiation of cell wall and cell membrane invagination in this organism.


Abnormal septation and inhibition of sporulation by accumulation of L- -glycerophosphate in Bacillus subtilis mutants. Accumulation of l-alpha-glycerophosphate, in cells of Bacillus subtilis mutants lacking the nicotinamide adenine dinucleotide-independent glycerophosphate dehydrogenase activity, suppresses both growth and sporulation. After growth has stopped, the cells slowly develop one and later more asymmetric septa that are thicker than normal prespore septa and apparently contain too much cell wall material to allow further membrane development into forespores or spores. l-Malate prevents accumulation of glycerophosphate and restores sporulation of the mutant. Glucose or gluconate cannot resotre sporulation, because they still effect glycerophosphate accumulation via de novo synthesis. If that accumulation is blocked in a double mutant, which is unable to make glycerophosphate from or to metabolize it into Embden-Meyerhof compounds, then nonsuppressing amounts of glucose or gluconate can restore sporulation.


Autolysis of listeria monocytogenes.

Autolysis of listeria monocytogenes. Autolytic curves of five representative strains of Listeria monocytogenes are described. Of 24 strains so far examined, the majority are unstable in vitro.


Photoprotection by dipicolinate against inactivation of bacterial spores with ultraviolet light. The resistance of three types of Bacillus cereus T spores to ultraviolet radiation corresponded to their dipicolinic acid (DPA) content. Photoprotection against ultraviolet light was observed in DPA-containing spores and in DPA-less spores irradiated through calcium dipicolinate.


On the conservation of aminoacyl-transfer ribonucleic acid synthetase genes in Bacillus. None of the heterologous deoxyribonucleic acid from various bacilli was capable of transforming lysyl- and tryptophanyl-transfer ribonucleic acid (tRNA) synthetase mutants of Bacillus subtilis to wild type. It was concluded that there is little conservation of the aminoacyl-tRNA synthetases even though the tRNA cistrons are conserved genetic functions.


Volatilization of mercuric chloride by mercury-resistant plasmid-bearing strains of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Several bacterial strains carrying genes determining mercury resistance on naturally occurring plasmids will convert 10(-5)m Hg(2+) (chloride) to a form of mercury which is volatile and soluble in organic solvents. The volatilization activity is induced by exposure to HgCl(2).


Transformation and transfection in lysogenic strains of Bacillus subtilis 168. Strains of Bacillus subtilis lysogenic for either temperate bacteriophage phi105 or SPO2 were reduced to less than 1.0% of the level of transformation of the nonlysogenic strains. Strains lysogenic for both phi105 and SPO2 are virtually nontransformable, indicating that the effect of lysogeny is additive. Lysogenic cultures transfected at essentially wild-type levels with deoxyribonucleic acid (DNA) isolated from bacteriophages phi29 and SPO1. The residual transformation and transfection achieved by the lysogenic cultures changed dramatically during growth in SPII medium, whereas nonlysogenic strains remained competent for 5 hr in SPII medium. Despite a marked reduction in transformation, lysogenic cultures initially irreversibly bound as much DNA as nonlysogenic cultures. After 60 min in SPII medium, there was a rapid decrease in the capacity of lysogenic cells to bind DNA irreversibly. These results, as discussed, indicate that the inhibition of transformation is probably due to an alteration of the cell surface or a differential inactivation of bacterial genes after lysogenic conversion.


Increasing activity of germinating Bacillus subtilis spores to incorporate thymidine triphosphate into deoxyribonucleic acid after detergent treatment. The incorporation of (3)H-labeled thymidine triphosphate ((3)H-dTTP) into deoxyribonucleic acid (DNA) of germinated and then Brij 58-treated Bacillus subtilis spores was measured to study DNA replication activity of cells. The dTTP incorporation rate was very low in dormant spores, gradually increased as germination proceeded, and reached a level of the vegetative cell activity approximately 4 hr after the start of germination. This is in contrast to the DNA polymerase activity in the cell extract which remained at the same level throughout the germination period. The increase of the dTTP incorporation activity was inhibited by chloramphenicol or phenethyl alcohol. When these inhibitors were added after germination had proceeded, the elevated dTTP incorporation activity gradually decreased. Permeability to dTTP of spores germinated in the presence of chloramphenicol and then treated with Brij 58 was confirmed by (i) (3)H-dTTP incorporation into the treated spores following either electron or ultraviolet irradiation and (ii) release of radioactivity from the treated spores containing radioactively labeled DNA after deoxyribonuclease I treatment.


Occurrence of glucosamine residues with free amino groups in cell wall peptidoglycan from bacilli as a factor responsible for resistance to lysozyme. Analysis by dinitrophenylation techniques revealed the occurrence of significant amounts of glucosamine residues with free amino groups in the peptidoglycan component of cell walls isolated from Bacillus cereus, Bacillus subtilis, and Bacillus megaterium. A close correlation was demonstrated between the content of N-unacetylated glucosamine residues in the peptidoglycan component and the resistance of the cell walls to lysozyme. These lysozyme-resistant cell walls and peptidoglycan were converted into a lysozyme-sensitive form by means of N-acetylation with acetic anhydride. Thus, the occurrence of the N-unacetylated glucosamine residues in the peptidoglycan component accounts for the resistance of these cell walls to lysozyme. The N-unacetylated glucosamine residues were not found in a significant amount in the cell walls of Micrococcus lysodeikticus, Staphylococcus aureus, Streptococcus faecalis, Lactobacillus casei, or Lactobacillus arabinosus.


Biosynthesis of glycogen and starch in Cryptococcus laurentii. Cells of Cryptococcus laurentii, when grown in liquid culture on 2% glucose close to neutral pH, showed glycogen granules throughout the cytoplasm. Glycogen levels of C. laurentii cells reached maximal levels just before onset of stationary phase. Concomitantly, a sharp rise in total and specific activity of glycogen synthetase was observed. Conversely, glycogen phosphorylase reached its highest specific activity approximately 3 hr after the glycogen peaked and remained high until most of the endogenous glycogen was utilized. Uridine diphosphoglucose pyrophosphorylase activity was always an order of magnitude higher than glycogen synthetase during log phase, but fell off rapidly after the cells reached stationary growth. Kinetic properties of the glycogen synthetase showed that the enzyme is always activated by glucose-6-phosphate, although the degree of activation by glucose-6-phosphate was found to be somewhat variable. The accelerated uptake of glucose commencing with the onset of stationary phase is explained by the rapid formation of extracellular acidic polysaccharide, which continues as long as there is glucose in the medium. In cells grown at pH 3.4, where no detectable extracellular acidic polysaccharide was formed, glucose uptake drastically declined when the cells reached stationary phase. These cells also contained glycogen-like granules in the cytoplasm. The evidence presented indicates that these granules are in fact glycogen, and that its structure does not resemble that of the starch excreted by cells grown at acidic pH.


Analysis of isoaccepting transfer ribonucleic acid species of Bacillus subtilis: chromatographic differences between transfer ribonucleic acids from spores and cells in exponential growth. Differences between the transfer ribonucleic acid (tRNA) of spores and exponentially growing cells of Bacillus subtilis 168 were compared by co-chromatography on reversed-phase column RPC-5. This system gave excellent resolution of isoaccepting species in 1 to 2 hr using a 200-ml gradient. Two methods were used to extract spore tRNAs, a procedure using a Braun homogenizer and a pretreatment with dithiothreitol followed by lysis with lysozyme. Where changes were observed, column elution profiles of spore tRNAs were independent of the extraction method used. Three kinds of changes between the profiles of vegetative cell tRNA and spore tRNA were observed: (i) no change; phe-, val-, ala-, asp-, ileu-, pro-, met-, fmet-, and his-tRNAs, (ii) a change in the ratio of existing peaks; gly-, tyr-, leu-, ser-, thr-, aspn-, and arg-tRNAs, and (iii) the appearance or disappearance of unique peaks; lys-, glu-, and trp-tRNAs.


Host range and properties of the Pseudomonas aeruginosa R factor R1822. R1822, a plasmid specifying multiple drug resistances, has been transferred to a variety of species representative of related and unrelated genera. The host range of the plasmid includes Enterobacteriaceae, soil saprophytes, Neisseria perflava, and photosynthetic bacteria. With the acquisition of drug resistance(s), these strains became sensitive to a small, ribonuclease-sensitive bacteriophage, designated PRR1, isolated by enrichment from sewage.


Interactions of Tetrahymena pyriformis, Escherichia coli, Azotobacter vinelandii, and glucose in a minimal medium. A study was made of the food web formed from a protozoon, two bacteria, and a glucose minimal medium in chemostat culture. The system was also divided into simpler parts, first by omitting the protozoon to obtain a competition system, and then by omitting one or the other of the bacteria to obtain two food chains. In the competition studies, one bacterium was displaced by the other at all holding times used. In the food chain studies, sustained oscillations of the population densities of predator and prey developed at short holding times, and then changed to damped oscillations at longer holding times. In addition, the level of residual glucose remained high at long holding times. A new model of microbial growth is necessary to explain these results. In the food web studies, predation of the protozoon on the two bacteria stabilized the competition between the latter and allowed their coexistence in the same habitat. Thus, Gause's principle was circumvented.


Divalent cations in native and reaggregated mycoplasma membranes. The Mg(2+) content of membranes of several Mycoplasma and Acholeplasma species varied between 0.88 and 1.98 mug of Mg(2+) per mg of protein, depending on the species and on growth conditions. Ca(2+) could be detected only when it was added to the growth medium. The Mg(2+) content of isolated A. laidlawii membranes could be increased almost threefold by dialysis against 20 mm Mg(2+), whereas aggregated A. laidlawii membranes contained about six to eight times more Mg(2+) per mg of protein than the native membranes. This was taken to indicate that the molecular organization of the lipid and protein in the reaggregated membranes differs from that of the native membranes. Between 60 and 83% of the Mg(2+) in native and reaggregated A. laidlawii membranes was associated with the lipid fraction extracted with chloroform-methanol. The removal of over 80% of membrane protein by Pronase digestion did not release any significant amount of Mg(2+). Hence, most of the divalent cation appears to be bound to membrane lipids, most probably to phospholipids. Ethylenediaminetetraacetic acid released the bulk of Mg(2+) bound to the native and reaggregated A. laidlawii membranes, except for about 0.5 mug of Mg(2+) per mg of protein which was too tightly bound. Hence, a small but fairly constant amount of Mg(2+) is unavailable for chelation.


Properties of D-arabinose isomerase purified from two strains of Escherichia coli. d-Arabinose isomerase (EC 5.3.1.3) has been isolated from l-fucose-induced cultures of Escherichia coli K-12 and d-arabinose-induced cultures of E. coli B/r. Both enzymes were homogeneous in an ultracentrifuge and migrated as single bands upon disc electrophoresis in acrylamide gels. The s(20,w) was 14.5 x 10(-13) sec for the E. coli K-12 enzyme and 14.3 x 10(-13) sec for the E. coli B/r enzyme. The molecular weight, determined by high-speed sedimentation equilibrium, was 3.55 +/- 0.06 x 10(5) for the E. coli K-12 enzyme and 3.42 +/- 0.04 x 10(5) for the enzyme isolated from E. coli B/r. Both enzyme preparations were active wth l-fucose or d-arabinose as substrates and showed no activity on any of the other aldopentoses or aldohexoses tested. With the E. coli K-12 enzyme, the K(m) was 2.8 x 10(-1)m for d-arabinose and 4.5 x 10(-2)m for l-fucose; with the E. coli B/r enzyme, the K(m) was 1.7 x 10(-1)m for d-arabinose and 4.2 x 10(-2)m for l-fucose. Both enzymes were inhibited by several of the polyalcohols tested, ribitol, l-arabitol, and dulcitol being the strongest. Both enzymes exhibited a broad plateau of optimal catalytic activity in the alkaline range. Both enzymes were stimulated by the presence of Mn(2+) or Co(2+) ions, but were strongly inhibited by the presence of Cd(2+) ions. Both enzymes were precipitated by antisera prepared against either enzyme preparation. The amino acid composition for both proteins has been determined; a striking similarity has been detected. Both enzymes could be dissociated, by protonation at pH 2 or by dialysis against buffer containing 8 m urea, into subunits that were homogeneous in an ultracentrifuge and migrated as single bands on disc electrophoresis in acrylamide gels containing urea. The molecular weight of the subunit, determined by high-speed sedimentation equilibrium, was 9.09 +/- 0.2 x 10(4) for the enzyme from E. coli K-12 and 8.46 +/- 0.1 x 10(4) for the enzyme from E. coli B/r. On the basis of biophysical studies, both isomerases appear to be oligomeric proteins consisting of four identical subunits.


Levels of glycogen and trehalose in Mycobacterium smegmatis and the purification and properties of the glycogen synthetase. The levels of glycogen, free trehalose, and lipid-bound trehalose were compared in Mycobacterium smegmatis grown under various conditions of nitrogen limitation. In a mineral salts medium supplemented with yeast extract and containing fructose as the carbon source, the accumulation of glycogen increased dramatically as the NH(4)Cl content of the medium was lowered. However, levels of free trehalose remained relatively constant. Cells were grown in low nitrogen medium and were then shifted to medium containing high nitrogen. Under these conditions, there was a rapid accumulation of glycogen in low nitrogen, and this glycogen was rapidly depleted when cells were placed in high nitrogen medium. Again the concentration of free trehalose remained fairly constant. However, when cells were grown in low nitrogen medium with [(14)C]fructose and then transferred to high nitrogen medium with unlabeled fructose, the specific radioactivity (counts per minute per micromole) of the free trehalose fell immediately, indicating that it was being synthesized and turned over continually. On the other hand, the specific radioactivity of the glycogen and bound trehalose declined much more slowly, suggesting that these two compounds were not turning over as rapidly or were being synthesized at a much slower rate. Experiments on the incorporation of [(14)C]fructose into glycogen and trehalose indicated that cells in high nitrogen medium synthesized much less glycogen than those in low nitrogen. However, synthesis of both free trehalose and bound trehalose was the same in both cases. The specific enzymatic activities of the glycogen synthetase and the trehalose phosphate synthetase varied somewhat from one growth condition to another, but there was no correlation between enzymatic activity and the amount of glycogen or trehalose, suggesting that changes in glycogen levels were not due to increased synthetic capacity. The glycogen synthetase was purified about 35-fold and its properties were examined. This enzyme was specific for adenosine diphosphate glucose as the glucosyl donor.


Purification and properties of the adenosine diphosphoglucose: glycogen transglucosylase of Pasteurella pseudotuberculosis. The enzyme which catalyzes the transfer of glucosyl residues from adenosine diphospho(ADP)-glucose to glycogen has been partially purified from extracts of Pasteurella pseudotuberculosis. In contrast to other glycogen synthetases of this type, guanosine diphospho-glucose had about 5% of the activity of ADP-glucose as a glucosyl donor. Some other properties of the enzyme are described and compared to other bacterial glycogen synthetases.


Surface structure of intact cells and spheroplasts of pseudomonas aeruginosa. This report describes the ultrastructural features of Pseudomonas aeruginosa after freeze-etching of intact cells and enzymatically prepared spheroplasts. Freeze-etching of intact cells revealed two convex layers of the cell wall and particles within the hydrophobic interior of the cell membrane. Areas of the membrane free of particles were sometimes elevated in the form of rather large dome-shaped structures. Spheroplasts were formed from intact cells by the addition of trypsin to a reaction mixture of lysozyme and ethylenediaminetetraacetic acid. Spheroplasts contained the outer lipoid layer of the cell wall. It was possible to observe this cell wall layer in freeze-etch preparations of spheroplasts. The spheroplast membrane like that of intact cells was cleaved along a central plane to expose particles and particle-free areas.


Novel mutant impaired in cell division: evidence for a positive regulating factor. A novel, conditional, cell-division mutant from Agmenellum quadruplicatum strain BG1 is described. During rapid growth in dilute suspensions, cell division lags behind mass increase and the cells form filaments. These filaments spontaneously divide into unit cell lengths as the culture density increases. Other conditions that favor the accumulation of metabolic products in the medium antagonize filament formations. An 80% ethanol-water extract of dried, spent medium also restores the ability of filaments to divide into cells of unit length. Our results suggest that at least one chemical factor acting as a positive effector is involved in cell division.


Procedure for the isolation of mutants of Bacillus subtilis with defective cytoplasmic membranes. A procedure has been devised to isolate mutants of Bacillus subtilis with structurally defective membranes. The procedure used to screen for the mutants involved comparison of the stability of protoplasts of the mutant with those of the wild type in a medium of sufficient osmotic strength to stabilize wild-type protoplasts. Mutagenized cells were grown as clones on agar plates, and then replicated onto plates containing 0.5 m lactose, which is sufficient to stabilize wild-type protoplasts. The colonies on the lactose-containing plates were then treated with lysozyme to convert the cells to protoplasts. Colonies of wild-type protoplasts remained opaque; however, colonies of mutant protoplasts lysed and became clear. Twenty-nine osmotically fragile mutants were isolated in this manner; the membranes of several mutants were found to contain alterations in the composition of their proteins or lipids.


Experience with microbiological assay for folate using a chloramphenicol-resistant L. casei strain. Experience with a semi-automated L. casei folate assay using a chloramphenicol-resistant strain of organism is described. Control of pH of medium and incubation time was required. The normal range for serum folate was 2 to 16 ng/ml and red cell folate 200 to 600 ng/ml.


Latex agglutination in the diagnosis of meningococcal meningitis. Group-specific polysaccharides of Neisseria meningitidis groups A and C have been demonstrated by means of a rapid, sensitive slide agglutination test with latex particles coated with antibodies. In this manner, the diagnosis can be made from the cerebrospinal fluid of patients with meningococcal meningitis caused by serogroups A or C even when the culture is negative. The method appears to be more sensitive than countercurrent immunoelectrophoresis and less elaborate.


Effects of Ca-free and EDTA-containing tyrode solution on transmembrane electrical activity and contraction in guinea pig papillary muscle.


Modulation of avoidance behavior in squirrel monkeys after chronic administration and withdrawal of d-amphetamine or  -methyl-p-tyrosine.


Rh "null" is not always null.

Rh "null" is not always null.


Gonococcal arthritis: a report of six cases. Six cases of gonococcal arthritis are described. Three presented during the initial "bacteremic stage" with polyarthralgia, fever, skin lesions and sterile synovial fluid. Two presented during the "septic joint stage" with positive synovial culture, and one presented during the "stage of residual deformity". Transient electrocardiographic changes were noted in two of the six cases. All responded to antibiotic therapy. One required additional surgical intervention.The condition is common, coinciding with the rising incidence of symptomatic and asymptomatic gonorrhea. Gonococcal infection must be considered in the differential diagnosis of migratory polyarthralgia, tenosynovitis, oligoarthritis, fever or unusual skin lesions. Criteria for diagnosis, lines of treatment and relevant literature are reviewed.


Cutaneous infections due to Corynebacterium diphtheriae. Toxigenic Corynebacterium diphtheriae was grown from skin lesions of 44 indigent patients seen at the emergency or out-patient departments of this hospital, 43 of them within the last 16 months of the study period. In all cases staphylococci or hemolytic streptococci were also present in the wounds. An increase in the incidence of clinical diphtheria occurred in the few months preceding and overlapping the period of recognition of the cutaneous infections. The gravis strains, which accounted for the majority of the infections, were sensitive to erythromycin and to penicillin, but were relatively resistant to cloxacillin.


U.S. politicians proposing a variety of federal health care programs; price tags 10 dollars to 80 billion dollars.


Angiographic findings in two cases of generalized neurofibromatosis.


Immune abnormalities associated with chronic mucocutaneous candidiasis.


Rosette formation by human thymocytes.

Rosette formation by human thymocytes.


Prostaglandin E 1  as a regulator of lymphocyte function. Selective action on B lymphocytes and synergy with procarbazine in depression of immune responses.


Hypothalamic thyrotropin releasing factor. Activity and pituitary responsiveness during development in the rat.


Chest wall tumors.

Chest wall tumors.


Low protein Rh immune globulin (Rh IgG). Purity, stability, activity and prophylactic value.


Electrophoretic precipitation and radial diffusion methods for assay of anti-D immunoglobulin preparations.


Characterization of Rh antibodies formed after incompatible pregnancies and after repeated booster injections.


Further studies of Xh, a serum protein antigen in man.


Serological studies on the Pr 1 -Pr 2  antigens using dog erythrocytes. Differentiation of Pr 2  from Pr 1  and detection of a Pr 1  heterogeneity: Pr 1h -Pr 1d .


Rhesus antibodies demonstrable only by enzyme technique.


Experimental and morphological study of cortical connections of the red nucleus.


Hemolytic anemia associated with Rh null  but not with Bombay blood. A hypothesis based on differing antigenic structures.


The production of high titre Rh (D) antibody in male volunteers.


Serum immunoglobulin levels in Trio and Wajana indians of Surinam.


Hunter's syndrome and Cooley's anaemia in the same patient. Effect of multiple transfusions.


Sweating in primates: secretion by adrenal medulla during exercise.


Algal viruses.

Algal viruses.


Early stress and later response to separation in rhesus monkeys.


X-rays and the retina of the primate fetus.


Differential susceptibility of rod and cone cells to argon laser.


Cell walls, lipids, and lipopolysaccharides of Pseudomonas species.


Objective and subjective parameters in angina pectoris (effect of beta-receptor blockade).


Role of glutathione in the morphogenesis of the bacterial spore coat. There is a marked increase in the half-cystine content of bacterial spores, especially the coat layers at the time of formation of the outer coat. When a cysteine auxotroph of Bacillus cereus T is grown on limiting cysteine, the spores contain the normal content of half-cystine, suggesting an alternate source. Glutathione appears to be such a supply of cysteine since it is hydrolyzed during sporulation and there are increased activities of the hydrolyzing enzymes at the same time. In addition, a cysteine auxotroph with a second alteration, a temperature-sensitive glutathione disulfide reductase, produces lysozyme-sensitive spores at 40 C. These spores appear to be defective in the formation of outer spore coat. During sporulation at 40 C, the double mutant accumulates oxidized glutathione which is a poor substrate for the hydrolytic enzymes. As a result, sporulating cells are deficient in half-cystines which are essential for outer spore coat morphogenesis. This alteration can be overcome by a shift to 30 C or by addition of cystinyl-pencillamine or cysteinyl-glycine to cultures sporulating at 40 C.


Effect of weak acids on amino acid transport by Penicillium chrysogenum: evidence for a proton or charge gradient as the driving force. A variety of weak acids at and below their pK(a) are potent inhibitors of transport in Penicillium chrysogenum. The effective compounds include sorbate, benzoate, and propionate (common antifungal agents), indoleacetate (a plant hormone), acetylsalicylate (aspirin), hexachlorophene, and a yellow pigment produced by the mycelia under nutrient-deficient conditions, as well as the classical uncouplers 2,4-dinitrophenol, p-nitrophenol, and azide. The results suggest that a proton gradient or charge gradient is involved in energizing membrane transport in P. chrysogenum. The unionized form of the weak acids could discharge the gradient by diffusing through the membrane and ionizing when they reach an interior compartment of higher pH. Experiments with 2,4-dinitrophenol and p-nitrophenol established that the ionized species are not absorbed by the mycelium to any great extent. The transport inhibitors also caused a decrease in cellular adenosine 5'-triphosphate (ATP) levels, but there was no constant correlation between inhibition of transport and suppression of cellular ATP. A decrease in aeration of the mycelial suspension had the same effect on transport and ATP levels as the addition of a weak organic acid. The effects on transport rates and ATP levels were reversible. The instantaneous inhibition of [(14)C]l-leucine transport by NH(4) (-) (and vice-versa) in nitrogen-starved mycelia at pH values of 7 or below can be explained by competition for a common energy-coupling system. The inhibition is not observed in carbon-starved mycelia in which the NH(4) (+) transport system is absent or inactive (but the general amino acid transport is fully active), or in iodoacetate-treated mycelia in which the NH(4) (+) transport system has been differentially inactivated. At pH values greater than 7.0, NH(3) and HPO(4) (2-) inhibit transport, presumably by discharging the membrane proton or charge gradient. Aniline counteracts the inhibitory effect of NH(3) and HPO(4) (2-) possibly by acting as a proton reservoir or buffer within the membrane.


Electric conductivity and internal osmolality of intact bacterial cells. Intact cells of Streptococcus faecalis and Micrococcus lysodeikticus were found to have high-frequency electric conductivities of 0.90 and 0.68 mho/m, respectively. These measured values, which reflect movements of ions both within the cytoplasm and within the cell wall space, were only about one-third of those calculated on the basis of determinations of the amounts and types of small ions within the cells. Concentrated suspensions of bacteria with damaged membranes showed similarly large disparities between measured and predicted conductivities, whereas the conductivities of diluted suspensions were about equal to predicted values. Thus, the low mobilities of intracellular ions appeared to be interpretable in terms of the physicochemical behavior of electrolytes in concentrated mixtures of small ions and cell polymers. In contrast to the low measured values for conductivity of intact bacteria, values for intracellular osmolality measured by means of a quantitative plasmolysis technique were higher than expected. For example, the plasmolysis threshold for S. faecalis cells indicated an internal osmolality of about 1.0 osmol/kg, compared with a value of only 0.81 osmol/liter of cell water calculated from a knowledge of the cell content and the distribution of small solutes. In all, our results indicate that most of the small ions within vegetative bacterial cells are free to move in an electric field and that they contribute to cytoplasmic osmolality.


Anthranilate hydroxylase from Aspergillus niger: evidence for the participation of iron in the double hydroxylation reaction. Evidence was obtained for the participation of iron in the double hydroxylation reaction catalyzed by anthranilate hydroxylase from Aspergillus niger (UBC 814). Omission of iron from the growth medium gave inactive preparations of anthranilate hydroxylase which could be reactivated by incubating the enzyme preparations with ferric citrate. The enzyme was susceptible to inhibition by metal chelating agents. The K(i) for o-phenanthroline, which inhibited the enzyme activity non-competitively with respect to anthranilate, was calculated to be 0.9 mM. The inhibition by o-phenanthroline was counteracted by ferric complexes such as ferric-ethylenediaminetetraacetic acid and ferric citrate. Anthranilate afforded protection against inhibition by o-phenanthroline.


Postmaturational cleavage of 23s ribosomal ribonucleic acid and its metabolic control in the blue-green alga Anacystis nidulans. Data are presented consistent with the notion that the 23s ribosomal ribonucleic acid (rRNA) of Anacystis nidulans undergoes specific endonucleolytic cleavage in vivo, to produce two fragments with molecular weights of 0.88 x 10(6) and 0.17 x 10(6) daltons. Cleavage occurred at random after 23s rRNA formation and was stimulated by light in this organism, an obligately photoautotrophic unicellular blue-green alga. The half-life of intact 23s rRNA was about 5 h in illuminated cultures and 10 h in unilluminated cultures. 3-(p-Chlorophenyl)-1, 1-dimethylurea, an inhibitor of photosystem II, retarded 23s rRNA cleavage in the light. The results are discussed in the context of recent reports of rRNA instability in a variety of eukaryotic and prokaryotic organisms.


Synthesis of ribosomal ribonucleic acid during sporulation of Bacillus subtilis. The incorporation of radioactive uracil into 50s and 30s ribosomal subunits and ribosomal ribonucleic acid (rRNA) was studied during the growth cycle of different sporogenic and asporogenic strains of Bacillus subtilis. It was found that partially synchronized cultures of the strains examined incorporated labeled uracil into the two ribosomal subunit species and rRNA during sporulation and during the stationary phase of the asporogenic strains. Kinetic studies have shown that, compared to vegetative cells, the percentage of uracil incorporated into the ribosomal subunits of cells taken 30 min after the end of exponential growth was decreased by about 25 to 35%. This decrease, however, appeared to be a general characteristic of stationary-phase cells and seems to depend on the nature of the sporulation medium and to some extent on the nature of the strain but not on the sp(+) or sp(-) phenotype of the strain. Moreover, by use of actinomycin D it was shown that the labeled uracil incorporated, in the presence of the drug, during the sporulation period was located in the ribosomal subunits (stable RNA). Based on these results, we concluded that during sporulation ribosomal genes are transcribed and consequently rRNA continues to be synthesized, although to a lesser extent than during vegetative growth. These results are discussed in the light of those obtained by Hussey et al.


Use of fluorouracil-uracil combinations to study growth accompanied by insufficient deoxyribonucleic acid synthesis in Bacillus cereus. 5-Fluorouracil (FU) at a concentration of 16 muM almost totally inhibited deoxyribonucleic acid (DNA) synthesis and cell division by Bacillus cereus, whereas growth continued at an exponential rate (25% of control for at least 3 h). In cultures simultaneously given 160 muM uracil (U) along with the FU, DNA synthesis still stopped, but cell division continued for one generation at the control rate and at a much slower rate beyond that; in the meantime, cell mass continued to increase at an essentially normal rate. The cells in cultures treated with FU or FU plus U were elongated and contained about half of the control content of DNA, with one nuclear area per cell instead of two. Loss of cloning ability, unlike mass increase, was always correlated with the continuing inhibition of DNA synthesis, in either FU- or U plus FU-treated cultures.


Manganese transport in Bacillus subtilis W23 during growth and sporulation. Manganese is accumulated in Bacillus subtilis by a highly specific active transport system. This trace element "pump" is insensitive to added magnesium or calcium and preferentially accumulates manganese in the presence of cobalt, iron, and copper. Manganese uptake in B. subtilis is inhibited by cyanide, azide, pentachlorophenol, and m-chlorophenyl carbonylcyanide hydrazone. The uptake of manganese follows Michaelis-Menten kinetics, and the net accumulation of manganese is regulated by increasing the V(max) after exposure to manganese-starvation conditions and by decreasing the V(max) for manganese uptake during growth in excess manganese. The K(m) remains constant during these regulatory changes in V(max). Manganese accumulated during growth is exchangeable for exogenous manganese and can be released from the cells by toluene (which causes leakage but not lysis) or by lysis with lysozyme. Two stages can be distinguished with regard to intracellular manganese during the process of growth and sporulation. During logarithmic growth, B. subtilis maintains a relatively constant internal manganese content, which is a function of the external manganese concentration following approximately a Langmuir adsorption isotherm. At the end of log phase, net accumulation of manganese slows. A second phase of net manganese accumulation begins at about the same time during sporulation as the accumulation of calcium begins. The manganese accumulated during growth and early sporulation is exchangeable and therefore relatively "free"; intracellular manganese is converted later during sporulation into a bound form that cannot be released by toluene or lysozyme.


Mutants of Bacillus subtilis temperature sensitive in the outgrowth phase of spore germination. Thirteen thermosensitive mutants of Bacillus subtilis defective in the outgrowth phase of spore germination were isolated. The spores of the mutants grow into vegetative cells at 35 C but not at 47 C, whereas the vegetative cells grow equally well at both temperatures. At 47 C all the mutant spores are able to initiate germination, but the process stops at an early phase of outgrowth in one strain and in a late phase in the other 12 strains. The spore of the latter gives rise to a swollen cell unable to divide. In all mutants, the normal phenotype is restored when the spores are grown in the presence of 20% sucrose or 2% NaCl. The synthesis of deoxyribonucleic acid and proteins does not seem to be altered in the mutants giving swollen cells. The mutants were grouped into three distinct genetic classes by transformation.


Fate of transforming deoxyribonucleic acid after uptake by competent Bacillus subtilis: nonrequirement of deoxyribonucleic acid replication for uptake and integration of transforming deoxyribonucleic acid. Studies on transformation of Bacillus subtilis using the inhibitor 6-(p-hydroxyphenylazo)-uracil show that deoxyribonucleic acid (DNA) replication is not required for the uptake and integration of donor DNA and genetic markers.


Morphological discontinuity of kinetosomes during the life cycle of Oxytricha fallax.


Hyperbaric cisternography: studies in an inanimate model and monkeys.


9 -Tetrahydrocannabinol: effects of EEG and behavior of rhesus monkeys.


Capacity of humoral immunologic response to controlled antigenic stimulation in certain malignant blood diseases. Production of precipitating antibodies.


Necrosis of gastric mucosa following orthostatic collapse in rabbits.


Nervous elements in the human colon of hirschsprung's disease (with comparative remarks on neuronal profiles in the normal human and monkey colon and sphincter ani internus).


Extracellular roughly granular material (ERGM) in human glomerular tufts. An ultrastructural study.


Sperm migration through the uterine cervix in the macaque during the menstrual cycle.


Pre-irradiation qualities of a parotid gland predicting the grade of functional disturbance by radiotherapy.


Predictability of the state of fetal oxygenation from a quantitative analysis of the components of late deceleration.


Spinal anesthesia and ephedrine in pregnant monkeys.


In vitro effects of Emko on Neisseria gonorrhoeae and Trichomonas vaginalis.


Studies on the binding of bacterial elongation factors EF Tu and EF G to ribosomes.


Cell-envelope changes in mutants of Citrobacter freundii with altered response to colicin A.


Mitochondrial-specific thymidine kinase.


Variations in activity of aminoacyl-tRNA synthetases as a function of development in Bacillus subtilis.


Deficiency of thyroid hormone and development of the fetal rhesus monkey. I. Effect on physical growth, skeletal maturation and biochemical measures of thyroid function.


Rabbit immunoglobulin A allotypic specificities. Localization to two papain fragments, fab 2   and fc 2  , of secretory immunoglobulin A.


Susceptibility of tularemia bacilli to the antagonistic action of cocci.


The Meckel syndrome with limited expression in relatives.


The patient with angina. Medical management plus sympathetic, continuous care.


Comparative metabolism of radionuclides in mammals. 8. Retention of beryllium in the mouse, rat, monkey and dog.


Long-term treatment of multiple sclerosis with azathioprine. Nineteen patients with multiple sclerosis were treated with azathioprine for two years. There was no evidence that treatment prevented deterioration or reduced the number or severity of the relapses when compared with 24 control patients observed over the same period.


Kinetic analysis of calcium movements in cell culture. V. Intracellular calcium distribution in kidney cells.


Microbiological determination of drug partitioning. 3. Partition coefficient profiles of several antimicrobial agents.


Constituents of Mammea americana L. XII. Biological data for xanthones and benzophenones.


The effect of sedation and awakening on energy substrates and hormonal relationships in the subhuman primate.


Terminal neuromas treated with triamcinolone acetonide.


Bacteriophage T4 head maturation: release of progeny DNA from the host cell membrane. We have presented a new approach to studying bacteriophage T4 head maturation. Using a modified M-band technique, we have shown that progeny deoxyribonucleic acid (DNA) was synthesized on the host cell membrane throughout infection. This DNA was released from the membrane later in infection as the result of formation of the phage head; detachment of the DNA required the action of gene products 20, 21, 22, 23, 24, 31, 16, 17 and 49, known to be necessary for normal head formation. Gene products 2, 4, 50, 64, 65, 13 and 14, also involved in head morphogenesis were not required to detach progeny DNA from the membrane; the presence of the phage tail and tail fibers also was not required. DNA was released in the form of immature heads and initially was sensitive to deoxyribonuclease (DNase). Conversion to DNase resistance followed rapidly. The amount of phage precursors present at the time of DNA synthesis determined the time of onset and detachment rate of DNA from the M band as well as the kinetics by which the detached DNA become DNase resistant.


Prophage-mediated interference affecting the development of Bacillus subtilis bacteriophage phi e. Bacteriophage phie shows a reduced efficiency of plating on strains of Bacillus subtilis which are lysogenic for the temperate bacteriophage SP02. Although this phenomenon resembles prophage-mediated restriction observed in other bacteria, host-controlled modification of phie was not observed. Mutants of phie which plated with high efficiency on the lysogenic host were isolated.


Epidemiology of diphtheria in San Antonio, Tex., 1970.


Viral RNAs associated with ribosomes in Sindbis virus-infected HeLa cells. Virus specific RNA ribosome complexes were isolated by sucrose density gradient centrifugation of cytoplasmic extracts from HeLa cells infected at 42 C with an RNA(+) mutant (ts2) of Sindbis virus. Viral RNA-ribosome complexes were accumulated by infected cells treated with sodium fluoride and cycloheximide. The RNA-ribosome complexes were characterized by (i) their sensitivity to the action of ribonuclease or ethylenediaminetetraacetic acid, (ii) their density in cesium chloride gradients, and (iii) presence of host ribosomes and viral RNAs. The viral RNAs were isolated and characterized. The results showed that two species of single-stranded RNAs (a 28s and 18 to 15s species) were associated with the complexes. Base composition analysis of the viral RNAs indicated that both species had a higher adenine content than the 42s or 26s forms of viral RNAs. The RNAs associated with the ribosome complexes were virus specific since they annealed with denatured double-stranded RNAs from the infected cells. Little or no 42S RNA was associated with the RNA-ribosome complexes. The results suggest that the 28s and 18 to 15s forms of RNAs may represent viral messenger RNAs.


Plastids of the yellow y-1 strain of Euglena gracilis.


Thermal gradients in the green monkey.

Thermal gradients in the green monkey.


Studies on Burkitt's lymphoma. I. A preliminary report on the presence of mycoplasma-like organisms in specimens examined by electron microscopy.


'Latent' pernicious anaemia in Delhi (India).


Haemoglobin Bart's and slow-moving haemoglobin x components in newborns. The homozygous state for the slow-moving X components in a Malay boy.


The failure of normal haemopoiesis in rats during the development of acute leukaemia.


Interaction of platelets with ascitic plasmacytoma in vitro.


In vivo effect of phytohaemagglutinin on mouse haemopoietic tissue.


Model systems for mono-oxygenases.

Model systems for mono-oxygenases.


Regain of biological activity of gonadotropin from choriocarcinoma.


Conformational effects of mercurials on rat liver ribosomes: a comparison between the unmasking of a shielded protein in the 60S subunit by phenyl mercurials and EDTA.


Induction, activation and inhibition of epoxide hydrase: an anomalous prevention of chlorobenzene-induced hepatotoxicity by an inhibitor of epoxide hydrase.


Plasmodium cynomolgi: the comparative infectiousness of individual rhesus monkeys.


Ascaridia galli (Nematoda): development and survival in gnotobiotic chickens.


Mouse splenic peroxidase and its role in bactericidal activity. Spleen cell suspensions from AKR and CD-1 mice contain peroxidase activity as determined by guaiacol oxidation. This activity is found predominately in the 20,000 x g pellet fraction of spleen cell homogenates. In the presence of H(2)O(2) and chloride ion at acidic pH, splenic peroxidase mediates the oxidation of d- or l-alanine to CO(2), NH(3), and acetaldehyde. The same reaction mixture without added amino acid can kill both gram-positive and gram-negative bacteria. The conditions for both reactions are similar. Both have an absolute requirement for H(2)O(2) and chloride ion, neither is active at neutral or alkaline pH, and both are inhibited by the sulfonic amino acid taurine. In these aspects, splenic peroxidase is qualitatively similar in its activity to myeloperoxidase (MPO) from polymorphonuclear leukocytes. It is quantitatively different from MPO in that the latter is more potent on a per guaiacol unit basis with respect to both amino acid oxidation and bactericidal activity. Still another quantitative difference is that splenic peroxidase requires 0.1 m NaCl for activity, whereas MPO functions with as little as 0.005 m NaCl. Splenic peroxidase and MPO both appear to differ qualitatively from horseradish peroxidase in that the latter enzyme does not mediate amino acid oxidation.


Isolation and characterization of a leptospiral type-specific antigen. The type-specific main (TM) antigen was extracted from the leptospiral cells of strain Kyoto of the Hebdomadis group by 90% phenol and was purified mainly by ethanol precipitation. The TM antigen was specific, forming one precipitin band by immunodiffusion and reacting by complement fixation test with anti-strain Kyoto serum; so far as has been tested, it does not appear to react with antisera of other serotypes. The sedimentation velocity of the TM antigen was 216S. Morphologically the TM antigen was filamentous in shape with a uniform width of 25 to 30 nm and various lengths from 50 nm to 1 mum. The infrared absorption pattern of the TM antigen generally resembled that of gram-negative bacterial endotoxin, and this fact suggests that the antigen is composed mainly of lipopolysaccharides. Galactose, rhamnose, xylose, arabinose, glucosamine, 13 kinds of acidic and neutral amino acids, lipids, and phosphorus were found in the TM antigen.


Hemagglutination assay of antibodies associated with pulmonary acariasis in rhesus monkeys (Macaca mulatta). Antibodies associated with infection by lung mites (Pneumonyssus simicola) in rhesus monkeys were measured by passive hemagglutination methods. Tanned human Rh negative O red blood cells or sheep red blood cells coated with soluble acarine antigens were used to detect mite-specific antibodies. Large quantities of host-specific mite antigens (P. simicola) were difficult to obtain, so free-living mite species such as Dermatophagoides farina, Acarina sheldonii, and Tyrophagus putrificiensis were also utilized. Although the best serological specificity and sensitivity were obtained with P. simicola and its derivatives (1:640), several free-living acarine species were useful in detecting antibodies to mites. Skin tests carried out on a limited number of animals were positive for both immediate and delayed hypersensitivity.


Chemical properties of the pili of Corynebacterium renale. Pili separated from the cells of Corynebacterium renale strain 46 (type II) by agitation at high speed in a homogenizer were purified by repeated cycles of ammonium sulfate precipitation, sonic treatment, and centrifugation. The preparation of purified pili formed a single antigen-antibody line in agar gel and showed an absorption maximum at 275 nm. The pili subjected to dodecyl sulfate-polyacrylamide gel electrophoresis formed a main band and corresponded to the molecular weight of 19,000. The fact that the total nitrogen of the amino acids of the pili was nearly equal to its nitrogen content, together with the absence of detectable carbohydrate, has led to the conclusion that the pili are protein. The pilial protein was composed of 20 amino acids. Preparations of pili which had been treated with 0.5 n NaOH, but not with 1 n HCl, no longer appeared filamentous and failed to form a precipitate with the antibody in agar gels. A comparison has been made of the amino acid composition and certain properties of the pili of C. renale and type I pili and F pili of gram-negative bacteria.


Recall of acquired cellular resistance in mice by antigens from killed Brucella. Mice infected with Brucella abortus 19 were challenged intravenously with Listeria monocytogenes. Spleen assays to determine the number of viable Listeria cells present revealed that these mice were highly resistant to Listeria when challenged on day 17 of the Brucella infection. Resistance was absent in mice challenged on the 5th day and was declining in mice challenged on the 33rd day. Resistance could not be detected by day 49 of the Brucella infection but could be recalled by the injection of antigens from smooth B. abortus 2308. Thus, extracted antigens appeared to be as effective in recall as the live cells used in earlier studies. Similar injections of extracts from rough B. abortus 45/20, or from B. ovis REO 198, were also effective in recalling resistance; this suggests that the smooth surface agglutinogen may be relatively unimportant in recall.


Relationship of serogroups of Neisseria meningitidis. II. R variants of Neisseria meningitidis. Meningococci isolated in primary cultures from nasopharyngeal carriers occasionally consisted of mixtures of smooth (S) and rough (R) strains. The R strains were separated from the S strains and their morphological and serological characteristics were studied. Some of these R strains reverted spontaneously to S strains which subsequently produced group-specific polysaccharide. Several R strains, grown in the presence of deoxyribonucleic acid from either an R strain of known parentage or an S strain, formed recombinants with serological group specificity.


Experimental tularemia in Macaca mulatta: relationship of aerosol particle size to the infectivity of airborne Pasteurella tularensis. Ninety-six Macaca mulatta were exposed to aerosol particles containing Pasteurella tularensis. Four different aerosols were employed that contained particle size distributions with median diameters of 2.1, 7.5, 12.5, or 24.0 mum. Size distributions were calculated only for those particles observed by phase microscope to contain organisms. Animals exposed to particles whose median diameters were either 2.1 or 7.5 mum were all infected and showed extensive infection of the lower respiratory tract, evidenced by large patches of consolidation with many necrotic foci on the surface. Death occurred in these animals 4 to 8 days after exposure. Monkeys exposed to 12.5- or 24.0-mum median diameter particles presented involvement of the cervical and mandibular lymph nodes, evidenced by swelling and abscess formation. Thirty-eight of the 45 animals in this group were infected. Those animals succumbing to the disease died from 8 to 21 days after exposure. The respiratory LD(50) values increased from 14 to 4,447 cells as the median diameter was raised from 2.1 to 24.0 mum.


Immunochemistry of the streptococcal group R cell wall polysaccharide antigen. The group R streptococcal group antigen has been shown to be a polysaccharide located at the surface of the cell wall of the organism. The antigen was extracted from cell walls in 0.05 n HCl or 5% trichloracetic acid at 100 C, from whole cells at room temperature in 0.85% NaCl or 0.1 m acetate (pH 5.0), and by sonic oscillation. The antigen is largely destroyed when extracted from whole cells in 0.05 n HCl at 100 C. Acetate is recommended for routine extraction. The antigen extracted by sonic treatment was separated into six immunologically active fractions on diethylaminoethyl-Sephadex. The fractions were found to possess a common antigen which exhibited similar properties on immunodiffusion and immunoelectrophoresis. The purified antigen did not react with any other streptococcal group antisera. Adsorption of group R serum with the antigen removed all antibodies against whole cell antigen extracts of R cells. Chemical and enzymatic analysis of three fractions showed that the antigen was composed of d-glucose, d-galactose, rhamnose, and glucosamine. No significant quantities of phosphorus, glycerol, ribitol, or muramic acid were present. Significant inhibition of the quantitative precipitin determination by d-galactose and stachyose indicated that galactose in terminal alpha linkage was the immunodominant hexose in the antigen. d-Glucose and d-glucosamine possessed a partial inhibitory activity. N-acetyl-d-glucosamine and l-rhamnose did not produce significant inhibition. The results indicate that the R antigen is an immunologically specific structure which serves as a reliable means of identification of these streptococci as a serological group.


Photic maculopathy in rhesus monkey. A light and electron microscopic study.


Classification of Neisseria meningitidis group B into distinct serotypes. I. Serological typing by a microbactericidal method. Neisseria meningitidis group B was subdivided into a number of different serotypes. A sensitive microbactericidal assay procedure was developed, and, with this method, over 10 different serotypes were provisionally established. The method by which distinct serotype designations were assigned is described. Almost half of the strains examined thus far contained a common serotype antigen which was distinct from the grouping antigen. Differentiation of group B meningococci into distinct types will aid in epidemiological studies and in the selection of appropriate strains for vaccines against group B meningococci.


Comparative effects of placebo and sustained-release nitroglycerin in anginal subjects. Preliminary report.


Biologic effects of endodontic procedures on developing incisor teeth. 3. Effect of débridement and disinfection procedures in the treatment of experimentally induced pulp and periapical disease.


Biologic effects of endodontic procedures on developing incisor teeth. IV. Effect of debridement procedures and calcium hydroxide-camphorated parachlorophenol paste in the treatment of experimentally induced pulp and periapical disease.


Effects of the administration of single amino acids on ribosome aggregation in rat liver.


Growth characteristics of microorganisms occurring in penicillin-treated Brucella abortus cultures.


Density in cesium chloride of the 27 nm "8FIIa" particle associated with acute infectious nonbacterial gastroenteritis: determination by ultra-centrifugation and immune electron microscopy.


The gross and biochemical effects of 1- -D-arabinofuranosyl cytosine on an actinobolin-resistant strain of Streptococcus faecalis.


Health education research project: All Saints and Mount Ayliff hospitals.


The effect of certain sulfenyl halides on the swelling of cells of Pseudomonas aeruginosa in salt solutions.


Statistical principles in public health field studies. Fifteenth report of the WHO Expert Committee on Health Statistics.


Diet and refractive characteristics.

Diet and refractive characteristics.


Natriuresis in the rhesus monkey after intracranial hypertension.


The fine structure of different bloodstream forms of Trypanosoma cruzi.


Behavioral and physiological studies of septal function and related medial cortical structures.


Analysis of bone remodelling in the otosclerotic stapes by means of tetracycline labelling.


Chromosome-sized DNA molecules from Drosophila.


Variant angina of Prinzmetal with normal coronary arteriograms. A variant of the variant.


Effects of nitroglycerin on responses of the systolic time intervals to exercise.


Angina pectoris in men. Prognostic significance of selected medical factors.


A double-blind comparison of fluocinonide and betamethasone valerate ointments.


Phlebitis associated with cephalosporins: cephapirin versus cephalothin.


Phenylprenazone in osteoarthritis.

Phenylprenazone in osteoarthritis.


Amoxapine: a double-blind evaluation of antidepressant activity.


Alpha methyldopa-chlorpromazine interaction in schizophrenic patients.


Detection of circulating DNA by counterimmunoelectrophoresis (CIE).


Plasma-level studies of chlorpromazine following the administration of chlorpromazine hydrochloride and chlorpromazine embonate in chronic schizophrenics.


Sterilization and therapeutic abortion counselling for the mentally retarded.


Experimental bedsonial arthritis.

Experimental bedsonial arthritis.


The amino acids of the cell wall of Mycobacterium tuberculosis var. bovis, strain BCG. Presence of a poly(L-glutamic acid).


Human lens hexokinase.

Human lens hexokinase.


Oculomotor control system.

Oculomotor control system.


The parasympathetic pathways to internal eye muscles.


The effect of calcium upon the reaggregation of bovine alpha crystallin.


An appraisal of nursing home care.

An appraisal of nursing home care.


Antigens of the rabbit ovary, oviduct and uterus.


Comparison of antigens in the ovary, oviduct and uterus of the rabbit and other mammalian species.


An iterative approach from the standpoint of the additive hypothesis to the dendrogram problem posed by molecular data sets.


Mechanisms of enzyme control as probed by equilibrium exchange rates: patterns of modifier effects with a two substrate, two product system.


Improved clinical definition of bladder carcinoma by preoperative external radiotherapy.


Bladder carcinoma treated by irradiation and surgery: interval report.


Induction of lymphoma in owl monkeys with heated, non-cytopathogenic Herpesvirus saimiri.


Nisseria infection in an appendectomy incision.


Purification and immunological characterization of mouse hepatic phosphoenolpyruvate carboxykinase.


Changes in glucagon level associated with anxiety or stress.


The type of research the profession needs.


The origin of subendothelial cells in developing coronary collaterals. A cytochemical approach.


Cell renewal in aortic necrosis following orthostatic collapse. An experimental autoradiographic study in the rabbit using  3 H-thymidine.


Quantitative relations between osteoblasts and osteoid in primary hyperparathyroidism, intestinal malabsorption ad renal osteodystrophy.


Case report on musculo-membranous pyloric bands.


Eye movements in systemically paralyzed macaque monkeys.


Selective media for faecal streptococci enumeration in water and sewage.


Regression of intercoronary collateral vessels in mongrel dogs after coronary bypass grafting.


Peak expiratory flow rates in chronic asthma. A gauge of progression and improvement.


Cromolyn treatment of asthmatic children.


Antibiotic treatment of asymptomatic bacteriuria.


Protracted diarrhea of infancy treated by intravenous alimentation. I. Clinical studies of 16 infants.


Protracted diarrhea of infancy treated by intravenous alimentation. II. Studies of small intestinal biopsy results.


Antimicrobials in the treatment of acute otitis media. A second clinical trial.


Electroencephalographic and blood chemistry responses to galanthamine hydrobromide in epileptic volunteers.


Immunologic studies of patients with pulmonary emphysema.


The occurrence of virus, interferon, and circulating antibodies in mice after experimental infection with Junin virus.


Virus particles and antigens in experimental orf scabs.


Persistent Borna virus infection in adult hamsters.


Spectroscopic characterization of ferredoxin from the blue-green alga Microcystis flos-aquae.


Studies on extracellular proteolytic enzymes from Staphylococcus aureus. I. Purification and characterization of one neutral and one alkaline protease.


Studies on extracellular proteolytic enzymes from Staphylococcus aureus. II. Isolation and characterization of an EDTA-sensitive protease.


ATP:UMP-CMP phosphotransferase from Tetrahymena pyriformis. II. Kinetic studies and reaction mechanism with UMP.


Effect of acetylation of Bacillus subtilis  -amylase on the kinetics of heat inactivation.


Comparison of two adrenergic beta-receptor blocking agents, alprenolol and propranolol, in treatment of angina pectoris.


Rheumatoid factor in hypertension.

Rheumatoid factor in hypertension.


Rh immunization in ruptured tubal pregnancy.


Function and distribution pattern of human T lymphocytes. II. Presence of T lymphocytes in normal humans and in humans with various immunodeficiency disorders.


Antibodies to human collagen in subjects with selective IgA deficiency.


A simple method for the rapid production of atherosclerosis in rats.


George Dimitrov about care of public health.


Cytological and cytochemical studies of the reactive connective tissue after interaction with some corticosteroids.


On the prognostic significance of the index liver-spleen activity--colloidal radioactive gold-- 198 Au in the scintigraphic examination of patients with hepatic cirrhosis.


Our experience in the treatment of infected pseudoarthroses.


Studies on thyroid immunity. VII. Splenectomy and monkey immune thyroiditis: thyroidal function and thyroxine transport.


Deoxyribonucleic acid-dependent ribonucleic acid polymerase from Drosophila melanogaster embryos.


M band protein. Two components isolated from chicken breast muscle. M band protein can be specifically extracted from fresh chicken breast muscle myofibrils suspended in 5 mM Tris-HCl pH 8.0. During discontinuous polyacrylamide gel electrophoresis the isolated protein separates into three bands which can be identified as two separate components (A, B) and a complex of the two. When partially purified fractions of the separated components are combined, an increase in the intensity of the band containing the complex can be shown. The polypeptide chain weights of the two components are 100,000 (A) and 40,000 (B) daltons as estimated by sodium dodecyl sulfate- (SDS-) polyacrylamide gel electrophoresis. Antibody prepared against total M band protein stains only the M band of the myofibril and is completely absorbed by M band protein. M band protein also absorbs the M band staining specifically from antibody which stains both I and M bands. Immunodiffusion data indicate that anti-M band is a mixture of two specific antibodies, one against each component.


Herpesvirus saimiri: enhancement of adenovirus-associated virus.


Antibody response and immunoglobulin levels in humans with acute or chronic Trypanosoma cruzi infections (Chagas' disease).


Tumor lipids: comparison of glyceryl ethers of mouse and insect tumors.


Feeding the baby of low birth weight.

Feeding the baby of low birth weight.


Cholesterol synthesis in the baboon.

Cholesterol synthesis in the baboon.


Formation of connective tissue proteins.


Adenovirus-associated (satellite) viruses.


Simian adenoviruses.

Simian adenoviruses.


The chicken embryo lethal orphan virus (CELO).


Supraependymal cells in recesses of the monkey third ventricle.


Agents stimulating gonadal function in the human. Report of a WHO scientific group.


Detection of antibody to hepatitis-associated antigen by indirect counterimmunoelectrophoresis.


Glottic cancer. An analysis of recurrence as related to dose, time, and fractionation.


Effectiveness of the carbenicillin-cephalothin combination against gram-negative bacilli.


Applications of the electron beam in the management of the lymphatics of the neck in head and neck cancers.


Supervoltage radiation therapy of the testicular tumors.


Peripheral intravenous feeding with isotonic amino acid solutions.


Insulin response to hemorrhagic shock in the intact and adrenalectomized primate.


Immunization of animals with anti-D immunoglobulin used for prophylaxis of hemolytic disease of newborns. 3. Antiglobulin immune antibodies obtained by immunizing rabbits with IgG anti-D immunoglobulin and their pattern in immunodiffusion and immunoelectrophoresis.


Levels of IgG, IgA and IgM immunoglobulins in acute viral hepatitis.


Synthetic antigens. I. Studies on immunogenicity of interpolymer of styrene and maleic anhydride and its derivatives.


Ammonia assimilation in blue-green algae.


Genetic control of riboflavin synthetase in Bacillus subtilis.


A method for isolating nuclei from Euglena gracilis.


Studies on the host phage systems of Corynebacterium hofmanni species.


Detection, characterization and diagnostic significance of human pregnancy-associated glycoproteins.


Observations on the regulation of uricase activity during development of Drosophila melanogaster.


Dosage compensation in Drosophila melanogaster triploids. I. Autoradiographic study.


Tandem duplications in Drosophila melanogaster. I. Interchromosomal effects of a heterozygous tandem duplication.


Alternative measurements of the metabolic component of the acid-base status in acute clinical situations.


The measurement of bidirectional sodium fluxes across the intestinal wall in man using whole gut perfusion.


Bacteriology of calculous cholecystitis.


A finite model for ciliated micro-organisms.


Nature of lymphocyte-tumor interaction. A general method for cellular immunoabsorption. The binding of sensitized lymphocytes to tumor cells that leads to tumor cell lysis in vitro has been investigated using poly-L-lysine-fixed tumor cell monolayers and lymphocytes obtained from the anatomical site of tumor allograft rejection. The results show that magnesium is an important prerequisite for this interaction and that the extent of lymphocyte-tumor cell binding depends upon temperature as well as pH. Binding can occur in the absence of serum, although serum factors are necessary for the completion of the cytolytic process. The poly-L-lysine technique is applicable to the formation of confluent monolayers with virtually any normal or neoplastic cell type, including those that are otherwise nonadherent to surfaces. Cells immobilized by this technique can be used for the specific immunoabsorption and subsequent recovery of effector lymphocytes sensitized against transplantation or tumor cell antigens.


The role of the fibrous components and ground substance in the mechanical properties of biological tissues: a preliminary investigation.


Long term anticonvulsant therapy and cerebellar atrophy.


Respiratory reactions to B. subtilis enzymes in detergents.


Inhibitors of Forssman guinea pig "anaphylaxis".


Effects of ascorbate on contractile activity of ileal smooth muscle.


Diabetic ketoacidosis in childhood.

Diabetic ketoacidosis in childhood.


Asymptomatic gonorrhoea in women: a prevalence survey in Sydney.


Exchange transfusion on children in Jakarta.


Effect of dipicolinic acid on the ultraviolet radiation resistance of Bacillus cereus spores.


Trimethoprim-sulphamethoxazole and folate metabolism.


Isolation of Mycobacterium tuberculosis from chronic suppurative otitis media.


Adenohypophysial growth and binding of thyroxine by adenohypophysial proteins in vitro.


The postoperative intensive care after cardiac surgery in the neonate.


Isolation of a type A influenza virus from an Australian pelagic bird.


Studies on the origin of pandemic influenza. 3. Evidence implicating duck and equine influenza viruses as possible progenitors of the Hong Kong strain of human influenza.


Proteins specified by group B togaviruses in mammalian cells during productive infections.


A pilus-dependent Pseudomonas aeruginosa bacteriophage with a long noncontractile tail.


Single cell analysis of saturation discrimination in the macaque.


Schistosomiasis control. Report of a WHO expert committee.


Serum gold. I. Estimation by atomic absorption spectroscopy.


Immunochemical evidence for the transport of neurophysin in the hypothalamo-neurohypophysial system of the dog.


Monitoring serum gold values to improve chrysotherapy in rheumatoid arthritis.


81 Br-NMR studies of carbonic anhydrase.


On the site of electron donation to the photosynthetic electron transport chain by 1,5-diphenylcarbazide.


The purification of human serum   1 -antitrypsin by affinity chromatography on concanavalin A.


Fatty acid composition and level of diacylglycerols and phosphoglycerides in brain and retina.


The bond hydrolyzed by cardiolipin-specific phospholipase D.


Acceleration of gluconeogenesis following inhibition of protein synthesis in Tetrahymena pyriformis.