Galactoside accumulation associated with ion movements in Streptococcus lactis.


Perfusion of isolated renal tubules.

Perfusion of isolated renal tubules.


Visualization of tubular interspaces in the kidney with the aid of lanthanum.


Long term effects of fusidic acid on bacterial protein synthesis in vivo.


Existence and inhibition of hydrolytic enzymes attacking paramyosin in myofibrillar extracts of Mercenaria mercenaria.


An adenosine triphosphate-dependent stabilization of proteolytic activity in heterolysosomes. Evidence for a proton pump. 1. Homogenization of mouse kidneys or livers in 0.25m-sucrose buffered with tris-acetate, pH7.3, resulted in a decreased rate of proteolysis within isolated heterolysosomes containing injected (125)I-labelled albumin when these particles were incubated at 35 degrees C. Proteolysis in mouse kidney or liver heterolysosomes isolated from homogenates made in 0.25m-sucrose buffered at pH7.3 was stimulated by pH5 buffer or by additions of ATP. 2. A greater inhibition of proteolysis was produced by including bicarbonate or pH8 borate buffers in the incubation media, and this inhibition was also reversed by ATP. 3. Other nucleoside triphosphates were not as effective as ATP, but GTP and ITP were more effective than CTP or UTP. ADP, AMP, or adenosine 3':5'-cyclic monophosphate were completely without effect. 4. Although ATP prevented some heterolysosome breakage in media containing bicarbonate, the primary effect appeared to be to promote proteolytic activity. 5. These observations are consistent with the presence of a proton pump in the heterolysosome membrane, which functions to maintain intralysosomal pH in alkaline media.


Inhibition of bacterial DNA replication in vitro by 6-(p-hydroxyphenylazo)-uracil: selective interference with the function of deoxyguanosine nucleotides.


Relation between aminoacyl-tRNA stability and the fixed amino acid.


Binding of rat liver ribosomes to nuclear complementary RNA.


Bacterial growth in albumen taken from the eggs of domestic hens and waterfowl.


Effects of aflatoxin B 1  on human skin lipids metabolism--a site of action on 1- 14 C-acetate incorporation.


Ionized calcium in exchange transfusion with THAM-buffered ACD blood. In 20 exchange transfusions with THAM-buffered ACD blood 5 ml. of 2% calcium gluconate (8 mg. elemental calcium) was injected after each 100 ml. of blood exchanged. Plasma ionized calcium decreased significantly during the procedure, although after each injection of calcium gluconate, levels returned briefly to normal. Ten minutes after the end of exchange ionized calcium had returned to pre-exchange levels and remained there until at least 30 mins. postexchange.Total calcium also increased significantly. Short periods of extreme hypercalcemia (between 7 and 8 mEq./l.) were noted after each injection of calcium gluconate.The amount of calcium gluconate was insufficient to counteract the calcium-chelating effect of citrate. If no heparinized blood is available we suggest adding heparin and calcium chloride to THAM-buffered ACD blood to avoid the repeated sudden fluctuations between low and high calcium ion activity.


Immunological evidence for the homogeneity of an ovine pituitary glycoprotein with dual gonadotropic activity.


Experimental allergic sialoadenitis. 3. Acute parotitis induced by instillation of antiserum to rat plasma into the glandular duct of rats.


Morphology of the primate optic nerve. 3. Fiber characteristics of the foveal outflow.


Morphology of the primate optic nerve. I. Method and total fiber count.


Morphology of the primate optic nerve. II. Total fiber size distribution and fiber density distribution.


Structural characterization of an anhydropenicillin and its stereochemical relationship to penicillins.


Effect of divalent cations and pH on intrinsic factor-mediated attachment of vitamin B 12 to intestinal microvillous membranes. Calcium, but not other divalent cations, is required for optimal uptake of intrinsic factor-bound (57)Co-labeled cyanocobalamin (IFB(12)) by microvillous membranes isolated from hamster ileal-absorptive cells. Chelation of divalent cations by disodium ethylenediaminetetraacetate (EDTA) promptly removes IFB(12) previously attached to microvillous membranes. High concentrations of CaCl(2) or MgCl(2) also markedly inhibit membrane uptake of IFB(12) and rapidly remove previously attached IFB(12). Similarly, reduction of pH to below 5.4 prevents membrane attachment of IFB(12) and removes virtually all IFB(12) already bound to microvillous membranes. The effects of calcium depletion, increased salt concentrations, and acidification on membrane uptake of IFB(12) were completely reversible. These findings are consistent with the concept that the formation of calcium salt bridges is essential for attachment of IFB(12) to the ileal-absorptive surface.


Potentiation of the contractile effects of norepinephrine by hypoxia. Hypoxia has been found to depress the concentration response curve of norepinephrine (NE) in isoalted cat papillary muscles. To investigate the effects of hypoxia in intact hearts, a heart-lung preparation was developed and maximum left ventricular dp/dt (max dp/dt) was measured at constant heart rate, preload, and after load. Left main coronary arterial flow (Q(e)) was measured with an electromagnetic flow probe. As arterial P(O2) decreased from 90 mm Hg (96% saturation) to 20-25 mm Hg (40% saturation) at constant P(CO2) and pH, no change in max dp/dt occurred and Q(e) increased 298%. In contrast to cat papillary muscles, the contractile responses to NE were augmented in hypoxia. The NE dose-response curves shifted to the left. No deterioration of contractility occurred after exposure to NE. In contrast, the chronotropic response was unaltered in hypoxia. Dose-response curves to isoproterenol also were shifted to the left in hypoxia, but responses to paired pacing were unchanged. The responses to NE under oxygenated conditions were unaltered by mechanically increased coronary flow or by increased coronary flow with nitroglycerin. Although the mechanisms responsible for these effects are unknown, the results suggest that hypoxia may open previously nonfunctioning vascular channels and thereby allow more extensive exposure of beta adrenergic receptors to circulating catecholamines.


The effect of azathioprine on gammaglobulin synthesis in man. The effect of azathioprine therapy on gammaglobulin synthesis was evaluated in nine patients with rheumatic disorders. The rates of synthesis of IgG and IgM were calculated before and after 4 months of azathioprine treatment using turnover studies of radioiodinated gammaglobulins. The synthesis of IgG decreased 33.4% of the initial IgG synthetic rate. Seven of nine patients showed reductions of IgG synthesis ranging from 16.8 to 74.1%. IgM production decreased from 24.5 to 74.2% in eight of nine patients with a mean reduction of 40.9%. Studies before and after a control period of 4 months of placebo in six patients revealed a mean increase in IgG and IgM synthesis. Serum immunoglobulin concentrations after 4 months of azathioprine treatment were not consistently reduced and did not parallel changes in immunoglobulin synthesis. Reduced gammaglobulin synthesis may play a role in the therapeutic action of this and other "immunosuppressive" drugs.


Presence of antigens and antibodies in the sera of chickens infected with Akiba caulleryi.


Quantitative observation on schizogony of Akiba caulleryi in chickens.


Experimental osteogenesis in periapical areas with decalcified allogeneic bone matrix.


Ossifying fibroma of the maxilla occurring with hyperthyroidism. Report of a case.


Insulin secretion in human protein-calorie deficiency.


Radioactive vitamin B 12 absorption studies: population distribution of plasma B 12 absorption and serum B 12.


Liver damage and estrogen therapy of prostatic cancer. Case report.


Carbonic anhydrase quantity in the blood of newborns.


A review of malaria work in Sierra Leone 1900 to 1964.


An aberrant form of Leucocytozoon infection in two quaker parakeets (Myiopsitta monachus Boddaert, 1783).


Babesiasis in a Malayan tapir (Tapirus indicus Desmarest, 1819).


The use of medical facilities for cerebrovascular disease patients in a county of Western Pennsylvania.


Observations on the vocal behaviour of free-ranging squirrel monkeys (Saimiri sciureus).


A comparative study on the utilization of phosphorus by pigs from various phosphates using oral and intraperitoneal administration of  32 P.


Rate of weight gain in chickens under the influence of folcysteine.


Oxygen availability in ischemic brain following hypocarbia and hypercarbia. Polarographical depth electrode recordings in evolving and completed experimental stroke in the monkey.


Retention and distribution of methylradiomercury in pigs.


The use of folcysteine for the control of embryo mortality in pigs. II. Results of large-scale experiments.


Metabolic responses to norepinephrine in hypocalcaemic sheep.


A critique of parenteral alimentation with respect to amino acid metabolism.


The penis and prepuce of the one-humped camel (Camelus dromedarius).


Juvenile xanthogranuloma.

Juvenile xanthogranuloma.


Cholestasis associated with long-term parenteral hyperalimentation.


The influence of secondary structure on the translation of ribosomal RNA.


Carcinogen-induced tRNA methylase activity in yeast cells.


L-Asparaginase from the BCG strain of Mycobacterium bovis. I. Purification and in vitro immunosuppressive properties.


Syntheses and antimicrobial activities of N-heterocyclic- -mercaptocinnamamides and related compounds.


Cerebral vasodilator therapy in stroke.

Cerebral vasodilator therapy in stroke.


Current aspects of bacterial enterotoxins.


Electron microscopical appearance of neuronal lipofuscin using different preparative techniques including freeze-etching.


Sterile incubation apparatus for cholesterol solubility studies.


Histologic evaluation of formocresol and oxpara pulpotomies in rhesus monkeys.


Levels of salivary IgA in treated and untreated coeliac disease. Estimations of the concentration of secretory IgA have been performed on 152 specimens of saliva from 46 patients with adult coeliac disease. The results are compared with values observed in 20 normal subjects. The mean salivary IgA concentration was 9 mg% in 36 patients on a normal diet and 7.9 mg% in 21 patients on a gluten-free diet. In 20 normals the mean salivary IgA was 7.1 mg%. Nine patients were studied before and after gluten exclusion. Seven of these showed a marked fall in salivary IgA concentration after gluten was excluded.


Physical separation of colony stimulating cells from in vitro colony forming cells in hemopoietic tissue.


Complement-fixation reactions in schistosomiasis: quantitative studies.


Aeromycology of Gorakhpur. IV. Periodical fluctuation of aerospora.


Metabolism of the vitamin-A-transporting protein complex: turnover of retinol-binding protein, prealbumin and vitamin A in a primate (Macaca Irus).


Gel filtration properties of factors II, VII, IX, IX a , and X.


-Aminolevulinic acid synthetase activity of human bone marrow erythroid cells in various hematological disorders.


Osteochondral grafts in growing rabbits. Histological, autoradiographic ( 35 S-sulphate), microangiographic, microradiographic and fluorochromic studies.


Round "virus-like" extracellular particles in glomerular tufts. An electron microscopic study of 190 human renal biopsies.


Ultrastructural study of a calcitonin-secreting tumor. Typhology of the tumor cells and origin of amyloid.


The functional morphology of the kidneys and the hypothalamic neurosecretory system in rabbits with ischemic cerebral hypertension.


Clinical relevancy of deep body temperature, heart rate and respiratory rate in the goat.


Diagnostic studies on gonadal sperm reserves in buffalo bulls.


Age associated histomorphologic changes in the cerebral arteries of the dog.


Inactive factor VIII in hemophilia A and Willebrand's disease. A study of 117 cases.


Cytogenetic observations on different cell lines in Hodgkin's disease.


Leucocyte loss in haemodialysis.

Leucocyte loss in haemodialysis.


The effect of androgens on erythroid and granulocytic marrow recovery in the irradiated rat.


Acute superficial phlebitis in a patient with hemophilia A. Probably an iatrogenic effect.


Heteromorphic pair of metacentric chromosomes with fused arms and the Philadelphia chromosome in a case of acute myeloid leukemia.


Human antibody to Bacteroidaceae.

Human antibody to Bacteroidaceae.


Studies on the indigenous gastrointestinal flora of Guatemalan children.


Enteric bacterial interactions in insects.


Mechanisms of Shigella pathogenesis.

Mechanisms of Shigella pathogenesis.


Orthodontic movement and return of the root apex through the cortical plate in the macaca speciosa monkey.


The effectiveness of orthopedic forces in inhibiting mandibular growth.


Human fibrinogen free of factor XIII: the pursuit of biological zero.


Molecular structure of plasma transglutaminase precursor.


Hereditary aspects of fibrin cross-linking.


Comparative studies on the fibrin-stabilizing factors from human plasma, platelets and placentas.


Comparative studies on tissue transglutaminase and factor XIII.


Demonstration of factor XIII in human megakaryocytes by a fluorescent antibody technique.


Site of synthesis of plasma and platelet factor XIII.


Degradation of stabilized and nonstabilized fibrin clots by plasmin: an immunological study.


Transglutaminase and tumor growth.

Transglutaminase and tumor growth.


Some thoughts on the state in nature, biosynthetic origin, and function of factor XIII.


Epsilon-(gamma-glutamyl) lysine cross-links: determination in fibrin from normal and factor XIII-deficient individuals.


Phosphoenolpyruvate, a new inhibitor of glucose-6-phosphate dehydrogenase.


Detection of the principal protein target of a hepatic carcinogen.


Separation of the glucose-6-phosphate independent and dependent forms of glycogen synthetase from yeast.


-Lactalbumin and galactosyltransferase in rat serum and their relationship to milk secretion.


Characterization of the subunits of thymidylate synthetase.


Bupivacaine and lignocaine induced seizures in rhesus monkeys.


The resorting of spinal afferents as antecedent to the body representation in the postcentral gyrus.


Neural organization of somatic sensory representation in the spider monkey.


Tuberculosis. An old disease returns to main stream of medicine.


Nutritional and clinical responses of immobilized patients to a sterile milk-base feeding.


Determination of L-histidine in nanogram amounts and the question of its occurrence in mast cells.


Observations on the specificity of newer histochemical methods for the demonstration of carbonic anhydrase activity.


Body potassium measurements by whole-body counting: screening of patient populations.


Radiotherapy of advanced Hodgkin's disease with curative intent.


Updated Hodgkin's disease: B. Curability of localized disease. Comment: The mantle failure: The transdiaphragmatic extension.


Nutritional therapy for adults with renal disease.


Allergic bronchopulmonary aspergillosis in India.


Laboratory and clinical experience with clotrimazole (Bay b 5097).


Heavy-density particles of Australia antigen detected in a human plasma pool.


St. Peter's Hospital in Hamilton: an education and research center for geriatric care.


Intravenous hyperalimentation.

Intravenous hyperalimentation.


A serological screening test for kidney damage. Spontaneous tubular damage in a rat discovered by detection of kidney specific antigens in the urine.


Augmented noradrenaline release following nerve stimulation after inhibition of prostaglandin synthesis with indomethacin.


Isolation of two turkey influenza viruses in Oregon.


A modified direct complement-fixation test for detection of Marek's disease antibodies in chicken serum.


LDH and LDH isoenzymes in amniotic fluid in normal and complicated pregnancy.


Resources for optimal care of congenital heart disease.


Acquisition and coding of analogue physiologic data for digital analysis.


Pneumonic aspergillosis in poults caused by Aspergillus flavus.


Characterization of an intracellular protease in B. subtillus during sporulation.


Biosynthesis of the phosphatidyl diglucosyl diglyceride of Streptococcus faecalis (ATCC 9730) from diglyucosyl diglyceride and phosphatidyl glycerol or diphosphatidyl glycerol.


Isolation and characterization of mutants of Escherichia coli K-12 which leak  -galactosidase.


Studies on rat-liver ribosomes, ribosomal subparticles and RNA fixed with formaldehyde.


Studies on the heterogeneity of mouse-globin messenger RNA.


Serological studies of Haemophilus influenzae. I. Extracellular antigens of capsulated strains, types a to f, and two non-capsulated variants.


Time course of macromolecule synthesis in human lymphocyte cultures stimulated by antilymphocyte globulin and phytohemagglutinin.


Transplantation of cells from bursa of Fabricius into surgically bursectomized chicks.


Reactions of procaine amide-induced antinuclear antibodies with fractions derived from calf thymus nuclei.


Tissue specific isoantigens in the dog prostate.


Cryo-immunology. Antibody response to epididymis freezing in the rabbit.


Abortion in cattle associated with Bacillus cereus.


Pathogenicity of Bacillus cereus for pregnant ewes and heifers.


Purification of mycobacterial deoxyribonucleic acid. Impurities believed to be polysaccharides have been found in mycobacterial deoxyribonucleic acid (DNA) preparations. Agar-gel diffusion of the DNA preparations against concanavalin A indicated the presence of three polysaccharides and was used to follow the purification procedures. The polysaccharides appeared to be the same for all strains studied. Precipitation of DNA with cetyltrimethylammonium bromide was used to separate impurities from some DNA preparations. The presence of the contaminants was found to affect markedly the determination of the guanine plus cytosine content according to a method dependent on the ratio of absorbancies at 260 and 280 nm; the impurities did not affect the determination by the method of thermal denaturation. The presence of a DNA-polysaccharide complex is suggested.


Genetic analysis of phenylalanine-responding mutants of Pseudomonas aeruginosa. A transduction analysis of phenylalanine-responding mutants of Pseudomonas aeruginosa revealed the existence of six unlinked genes. Enzyme assays showed that one gene was involved in the terminal production of phenylalanine (chorismate mutase), and the remaining five genes were involved in the common pathway of aromatic amino acid biosynthesis.


Saliva-induced aggregation of oral streptococci. Cells of several species of oral microorganisms have been shown, in earlier studies, to be aggregated by saliva. In the present study some of the basic properties of the aggregation system are examined. The observation is made that the saliva-induced aggregates of Streptococcus sanguis and S. mitis can be dissociated to stable particles which consist of about 100 cells and have a median diameter of about 4.5 mum. It is proposed that these are subunits, or core aggregates, of the large primary aggregates. Counts of the core aggregates can be taken as a precise and accurate measure of aggregation. Experiments based on this procedure show that the aggregation of S. sanguis is maximal at 10 C and at 1 meq of Ca(2+) ions per liter and is not affected by a change in pH between 3.9 and 8.7 or by a change in the phase of growth of the microorganisms. Core aggregates diminish in number with prolonged incubation, suggesting that the aggregating factors break down with time. Formalinized cells yield stable aggregates. However, with Formalinized cell aggregation is maximal between 20 and 30 C and proceeds in the absence of calcium ions. Evidence is presented that whole saliva contains separate aggregating factors for S. sanguis and S. mitis. The factors differ in their affinity for intact cells and for hydroxyapatite and differ in their stability to dialysis. These findings suggest that many different aggregating factors exist in saliva, each of which may be capable of interacting with cells of one or several bacterial species.


Action of phospholipase A 2  and phospholipase C on Bacillus subtilis protoplasts. Protoplasts prepared from Bacillus subtilis by lysozyme digestion lysed in the presence of pure pancreatic phospholipase A(2). The phospholipids cardiolipin, phosphatidylethanolamine, phosphatidylglycerol and lysylphosphatidylglycerol, which are present in the membrane, are degraded by phospholipase A(2) only after removal of the cell wall, giving free fatty acids and lyso derivatives. The four phospholipids are hydrolyzed equally well at a given enzyme concentration. Differences in the phospholipid composition of the protoplasts were obtained by variations in the growth medium, time of harvesting, and preincubation time with lysozyme. The extent of hydrolysis appeared to depend on the initial phospholipid composition. A relative increase in acidic phospholipids in the membrane facilitated the action of phospholipase A(2), whereas the rate of hydrolysis was diminished when protoplasts were tested which contained a relatively high amount of positively charged phospholipid. Pure phospholipase C from B. cereus preferentially hydrolyzed phosphatidyl-ethanolamine in the B. subtilis membrane. More than 80% of this phospholipid was converted into diglyceride, whereas only 30% of the cardiolipin was hydrolyzed. Such a loss of phospholipids, however, was not followed by lysis of the protoplasts. Liposomes were prepared from the lipid extracts of B. subtilis and incubated with both phospholipases. The hydrolysis pattern of the phospholipids in these model membrane systems was identical to the hydrolysis pattern of the phospholipids in the protoplast membrane. Phospholipase A(2) hydrolyzed all the phospholipids in the liposomes equally well, whereas phospholipase C preferentially degraded phosphatidylethanolamine.


Template specificity of ribonucleic acid polymerase in asporogenous mutants of Bacillus subtilis. Asporogenous mutants of Bacillus subtilis were examined for the change in template specificity of ribonucleic acid (RNA) polymerase characteristic of wild-type cells undergoing sporulation. Mutants blocked at stages II, III, and IV showed a changed specificity of the enzyme after the end of growth and were in this respect indistinguishable from the wild type. The RNA polymerase of eight stage-zero mutants (out of nine tested) which possess mutations that map at six distinct loci retained the template specificity of vegetative cells.


Distribution of neuraminidase and n-acetylneuraminate lyase activities among corynebacteria, mycobacteria, and nocardias. In Corynebacterium diphtheriae and closely related neuraminidase-producing corynebacteria, we have found an N-acetylneuraminate (NAN) lyase activity which cleaves NAN into N-acetyl-d-mannosamine and, presumably, pyruvate. In vitro, these lyases can be shown to synthesize NAN. A survey of representative corynebacteria, "plant pathogenic corynebacteria," mycobacteria, and nocardias revealed that only those corynebacteria closely related to C. diphtheriae exhibited both neuraminidase and NAN lyase activities.


Comparative effect of oxygen and nitrate on protoporphyrin and heme synthesis from delta-amino levulinic acid in bacterial cultures. Pseudomonas aeruginosa and P. denitrificans accumulate more protoheme and considerably more protoporphyrin during anaerobic growth under denitrifying conditions than during aerobic growth. In Escherichia coli, the small accumulation of protoporphyrin and protoheme which occurs during anaerobic growth is slightly stimulated by nitrate and markedly stimulated by oxygen.


Heterofermentative carbohydrate metabolism of lactose-impaired mutants of Streptococcus lactis. Two mutants of Streptococcus lactis ATCC 11454 have been isolated which possess an impaired lactose-fermenting capacity; galactose utilization is also affected, but to a lesser extent. Although the Embden-Meyerhof-Parnas pathway is the major, if not the sole, pathway of carbohydrate metabolism in the three strains, the fermentation end products of the mutants are dramatically different from the typical homolactic pattern of the wild type. Under conditions of low oxygen tension and growth-limiting lactose concentrations, mutant strain T-1 produces largely formic acid, acetic acid (2:1), and ethanol rather than lactic acid. Aerated cultures produce acetic acid, CO(2) (1:1), acetyl-methylcarbinol, and diacetyl. When the mutants use galactose as an energy source, lactic acid is the major end product, but significant heterofermentative activity is observed. The aberrations responsible for the mutant phenotypes reside in the proteins which catalyze the transport and hydrolysis of galactosides. It is hypothesized that the impaired transport system of the mutants reduces the intracellular pool of glycolytic intermediates below that of the wild type. Since fructose-1, 6-diphosphate is an activator of lactic dehydrogenase in S. lactis, lactic acid production is reduced, and pathways leading to the formation of other products are expressed.


Murein and the outer penetration barrier of Escherichia coli K-12, Proteus mirabilis, and Pseudomonas aeruginosa. A penetration barrier operating outside the periplasmic enzyme penicillinase was studied in an ampicillin-resistant mutant of Escherichia coli K-12. Growth in the presence of lysozyme and sublethal concentrations of ampicillin partially opened the barrier. This could be recorded as an increased penetration of penicillin G, sodium cholate, and rifampin to their respective targets. Brief treatments with tris(hydroxymethyl)aminomethane-ethylenediaminetetraacetic acid and sodium cholate effectively impaired the barrier against penicillin and also caused leakage of penicillinase. Wild-type E. coli K-12, Proteus mirabilis, and Pseudomonas aeruginosa also showed an increased sensitivity to cholate after treatment with penicillins. Electron micrographs showed that lysis by cholate was due to a distortion of the cytoplasmic membrane causing a leakage of protein and RNA from the cells to the medium. Physiological data indicated that the increased sensitivity to cholate induced by growth in the presence of ampicillin or lysozyme was due to effects upon the murein. This was supported by measurement of the incorporation of (3)H-diaminopimelic acid. These results indicate that the murein sacculus either is a part of the penetration barrier or is responsible for holding the structure of the outer membrane together.


Turnover of phospholipids in an unsaturated fatty acid auxotroph of Escherichia coli. The membrane phospholipids of an unsaturated fatty acid auxotroph of Escherichia coli were found to undergo turnover. These phospholipids were excreted into the culture medium, and were replaced in the cell with newly synthesized phospholipids. Phospholipids of growing cells supplemented with elaidic acid underwent rapid turnover, while those of cells supplemented with oleate, or cis-vaccenate plus palmitoleate, underwent slow turnover. Starvation for required amino acids stimulated this turnover in the latter two cases. Protein was also lost from growing cells. However, after amino acid starvation this loss ceased while phospholipid turnover continued. Electron micrographs of growing cells indicated that large pieces of membrane-like material were separating from the cell surface.


Specific immunoassay for quantitative determination of human erythrocyte catalase.


An acyclic puromycin analog. 6-Dimethylamino-9-(2-hydroxy-3-(p-methoxyphenyl-L-alanylamino)propyl)purine.


Nucleoside peptides. 5. Synthesis of certain N-(5-uridineacetyl)amino acids from 5-uridineacetic acid.


Localization of immunoglobulin A in electrophoretic patterns of proteins from human parotid saliva.


Pericillinase inhibition.

Pericillinase inhibition.


Preliminary study of inhibitory effects of tetracyclines on membranous bone growth in rhesus monkeys.


Comparison of histological stained sections with culturing techniques in the evaluation of contaminated wounds.


Storage and excretion of DDT.

Storage and excretion of DDT.


Electron microscopic and histochemical evidence for a tubular innervation in the renal cortex of the monkey.


A comparison of the distribution volumes of inulin and ( 51 Cr)EDTA in man and nephrectomized dogs.


A simple method for the determination of glomerular filtration rate.


The effect of antimicrobial agents on bilirubin binding by human erythrocytes.


The effect of infection with adenovirus 2 on the transcription of cellular RNA.


Expression of diphtheria toxin genes carried by integrated and nonintegrated phage beta.


DNA replication and late protein synthesis during SP82 infection of Bacillus subtilis.


A host mutation affecting the synthesis of late proteins during infection of Bacillus subtilis by bacteriophage SP82.


Change involving a viral membrane glycoprotein during morphogenesis of group B arboviruses.


Inhibition of early viral ribonucleic acid synthesis in interferon-treated cells infected with frog polyhedral cytoplasmic deoxyribovirus.


Serial passage of a nuclear polyhedrosis disease virus of the cabbage looper (Trichoplusia ni) in a continuous tissue culture cell line.


Ferritin-conjugated antibody labeling of visna virus.


Hemodynamic effects of oxprenolol alone and combined with nitroglycerin in patients with ischemic heart disease.


Efficacy of a fluorescent-antibody procedure for identifying Bacillus cereus in foods. One hundred and seventeen strains of Bacillus were examined by the fluorescent-antibody technique by using the globulin fraction of serum prepared against spores of B. cereus T. All but one strain of the 59 B. cereus tested fluoresced at the exosporium surface. Fluorescent staining of B. anthracis, B. thuringiensis, and B. mycoides was also observed. Absorption of the globulin fraction with B. anthracis and B. mycoides resulted in the elimination of staining of these organisms. Absorption with B. thuringiensis ATCC 10792 removed antibodies reacting with 6 of the strains of B. thuringiensis tested. Absorption with B. thuringiensis var. galleriae removed antibodies against B. cereus to such a degree that the globulin fraction was unusable.


Properties of thymineless strains of Bacillus megaterium. Both Bacillus megaterium KM:T(-)R(1), a strain partially resistant to thymineless death, and strain KM:T(-), the parent strain, can satisfy their thymine requirement with either thymidine, 5-methyldeoxycytidine, or 5-methyluridine. Neither strain can use 5-methylcytosine, 5-hydroxymethylcytosine, 5-hydroxymethyluracil, or 5-aminouracil for this purpose. Strain KM:T(-)R(1) requires as little as 0.01 mM thymine for maximum growth, whereas strain KM:T(-) requires 0.10 to 0.20 mM thymine. Lysogenic KM:T(-)R(1) dies more rapidly in the presence of mitomycin C than the corresponding phage-sensitive strain. Unexpectedly, the lysogenic strain was found to be less sensitive to thymineless death than the phage-sensitive strain. Lysogenic KM:T(-)R(1) is induced by exposure to mitomycin C and by thymineless incubation. It is concluded that thymineless death occurs by a mechanism which is unrelated to phage induction and that a major lethal effect of mitomycin C is probably a consequence of phage induction.


Identification of the predominant volatile compounds produced by Aspergillus flavus. A culture of Aspergillus flavus grown on moistened wheat meal was homogenized with a blendor, and the resulting slurry was vacuum-distilled at 5 mm of Hg and 35 C. The aqueous distillate was collected in traps cooled to -10 to -80 C. The culture volatiles were extracted from the distillate with CH(2)Cl(2), and, after removal of the bulk of the solvent, the concentrated volatiles were examined by packed-column gas chromatography. Nineteen peaks were observed, and coupled gas chromatography-mass spectrometry was employed to identify the larger components. The compounds identified were: 3-methyl-butanol, 3-octanone, 3-octanol, 1-octen-3-ol, 1-octanol, and cis-2-octen-1-ol. The two octenols were the predominant compounds, and sufficient sample was trapped from the gas chromatograph for infrared analyses; this confirmed the mass spectral identifications and permitted the assignment of the cis designation to 2-octen-1-ol. Both oct-1-en-3-ol and cis-2-octen-1-ol are thought to be responsible for the characteristic musty-fungal odor of certain fungi; the latter compound may be a useful chemical index of fungal growth.


Polysaccharide produced by Anacystis nidulans: its ecological implication. An extracellular polysaccharide from Anacystis nidulans was extracted from cell-free medium. Analysis showed that the polysaccharide consisted of glucose, galactose, and mannose in the ratio of 60:14:20. The production of polysaccharide depends on the age of culture, the growth temperature, and the form of nitrogen available.


Use of a clonal line of porcine kidney cell cultures for primary isolation and vaccine studies with adenoviruses. The clonal line (Y15) of porcine kidney stable cells provided a recovery system for adenovirus T4 from specimens from adults with respiratory illnesses that was as sensitive as human embryo kidney cultures. Adenoviruses T7 from adults, and T1, 2, 3, and 5 from children could be readily isolated in porcine kidney cell cultures. The latter were useful for adenovirus vaccine studies in that infectivity titers of live virus vaccine and neutralization antibody responses after vaccination were equal to those obtained in human embryo kidney cultures.


Effect of physiological age and state on survival of desiccated Pseudomonas aeruginosa. Cells of Pseudomonas aeruginosa harvested after 6 hr, 24 hr, 7 days, 33 days, and 120 days of incubation at 36 C varied in their resistance to the deleterious effects of desiccation. The longest survival period observed was for cells from cultures incubated for 7 days. Cells of the exponential growth phase were the most susceptible. The addition of extracellular slime from 7-day cultures to suspending medium did not increase survival of cells from the exponential growth phase. No marked differences in survival time were observed for cells of similar physiological age from cultures incubated at 20, 36, and 42 C. In comparison with the laboratory strain, P. aeruginosa OSU 64, used in this study, strains isolated from natural habitats and subjected to a minimum of manipulation in the laboratory were not better adapted to withstand the stress of desiccation.


Chromogenesis mirabilis in Streptomyces griseus. A number of chromogenic Streptomyces, producing diffusible melanoid pigment on complex organic media, fail to form melanin pigment on conventionally used synthetic tyrosine agar. By means of our new melanin formation test, almost all the chromogenic streptomyces can now be detected in chemically defined medium. In contrast to ordinary chromogenic streptomyces, two streptomyces species of the International Streptomyces Project, S. griseus ISP 5236 and S. ornatus ISP 5307, produce melanin pigment only on synthetic tyrosine agar, without showing chromogenicity on complex organic media. From the results obtained with S. griseus ISP 5236 and S. phaeochromogenes ISP 5073, it was revealed that melanin formation by Streptomyces, in general, is inhibited by L-cysteine present in organic nitrogen sources incorporated into natural media. Most chromogenic species of streptomyces produce a higher level of tyrosinase and rapidly utilize L-cysteine in the culture media which result in the manifestation of good chromogenicity on natural media. Peculiarity of chromogenicity of S. griseus and S. ornatus might be due to the lower ability to produce tyrosinase and to utilize L-cysteine in the culture medium.


The effect of maternal malnutrition on the progeny in the rat. Studies on growth, body composition and organ cellularity in first and second generation progeny.


Adsorption of colloidal iron by bacteria. The adsorption of iron from a positive-iron sol by species of seven bacterial genera was examined by electron microscopy. All species precipitated the iron from the sol, and the bacterial cells became encrusted with iron. This was related to iron deposition in surface water supplies.


Concentrations of immunoglobulin in mammary secretion of ruminants during involution with particular reference to selective transfer of IgG.


Incidence of cysticercosis and sarcosporidiosis in sheep in south eastern Australia.


Epithelial basement membranes: the isolation and identification of a soluble component.


Mechanism of acidification of the mucosal fluid by the toad urinary bladder.


Calcium binding to the rat liver plasma membrane.


Estimation of serum folate levels by competitive protein binding assay.


The nature of crystallinity of  -crystallin preparation of bovine lenses.


Gas analysis and venous admixture in the patients with hepatic cirrhosis.


The lavsant in the reconstructive surgery of the knee joint.


Homoosseous-cartilagenous arthroplasty of the small joints of the hand.


Effectiveness of the measures against dust content in the copper smelting mines of Panagjuriste.


Electrolithotripsy.

Electrolithotripsy.


Professor D-R Todor Zahariev (on occasion of his 60th birthday).


Isolation and characterization of three minor alkaloids of Bocconia (Macleaya) cordata Willd.


Study of the alkaloid composition of Genista rumelica Vel. (first communication).


Completely intravenous nutrition following massive intestinal resection.


Stopped flow spectrophotometric studies of yeast enolase subunit interaction.


Cytoplasmic-type 80 S ribosomes associated with yeast mitochondria. I. Evidence for ribosome binding sites on yeast mitochondria.


The molecular basis of articular lubrication. I. Purification and properties of a lubricating fraction from bovine synovial fluid.


Phosphorylation of casein. Role of the golgi apparatus.


Isohomoaminopterin and isohomofolic acid. Synthesis and basic biochemical and biological properties.


Adrenal corticosteroids and the response to epinephrine or electrical stimulation of the enzymes of glycogen metabolism in rat skeletal muscle.


Colour and fluorescence of the optic disc.


On the mode of action of silver sulfadiazine.


Scanning electron microscope studies of vertebrate red cells.


Antiarthritic and immunosuppressive effects of Pseudomonas aeruginosa.


The psychopharmacology of "cannabis sativa": a review.


Retention of a sulfated polysaccharide (SPG) and attempts at modifying this retention.


"Nonsecretory" plasma cell myeloma: observations on seven cases with electron microscopic studies.


A group B analogue of subgroup A 3 .

A group B analogue of subgroup A 3 .


The growth of Pseudomonas in blood cultures.


Antibody screening with the autoanalyzer using donors' plasma in place of sera.


Automated serum calcium analyses corrected for dialysis effects.


Platelet agglutination induced by ethylenediaminetetraacetic acid in blood samples from a miniature pig.


An epidemiologic account of tuberculosis transmitted from man to monkey.


Tuberculosis: surveillance or control.

Tuberculosis: surveillance or control.


Investigation of cross-link formation in DNA by the alkylating cytostatic IMET 3106, 3393 and 3943.


The use of RNA labeled in vitro with iodine-125 in molecular hybridization experiments.


Responses of cat geniculate ganglion tongue units to some salts and physiological buffer solutions.


Metabolism and biochemistry of mycophenolic acid.


Pattern of 2-methyl-4-dimethylaminoazobenzene-binding proteins in the livers of partially hepatectomized rats and of continuously dye-fed rats in comparison with 3'-methyl-4-dimethylaminoazobenzene.


An abnormal orosomucoid in the plasma of patients with neoplastic disease.


The biological activities and detection of naturally occurring 12,13-epoxy-delta9-trichothecenes.


Influence of environmental factors on mycotoxin toxicity as evidenced by studies with aflatoxin.


Chest clearance of inhaled cobalt-60 oxide.


The measurement of californium-252 in vivo.


Impairment of the lung clearance mechanism by respiratory infection.


Diagnostic significance of plasma proteins: a new dimension in the clinical laboratory.


Improving nursing home care: national approach to long-term care.


A temperature sensitive mutant in Bacillus subtilis with an altered elongation factor G.


Protective heterologous immunity between Plasmodium atheruri and other Plasmodium spp. and Babesia spp. in mice.


Immunity to malaria and piroplasmosis in mice following low level infections with Anthemosoma garnhami (Piroplasmea: Dactylosomidae).


Absence of immunity between Trypanosoma musculi and intra-erythrocytic protozoa in mice.


Culture of Plasmodium falciparum in vitro: a subculture technique used for demonstrating antiplasmodial activity in serum from some Gambians, resident in an endemic malarious area.


A method for the quantitative microbiological check of heat decontaminators.


Diphtheria carriers among school children in Athens.


Acidification of urine by the isolated urinary bladder of the toad.


Circulatory transport of L-glutamine in fasted mammals: cellular sources of urine ammonia.


Inherited defects in thyroid hormone synthesis.


Absorption of antibiotics: influence of metoclopramide and atropine on serum levels of pivampicillin and tetracycline.


Ventricular arrhythmias associated with the ingestion of alcohol.


Radiation treatment of invasive cancer of the cervix in Queensland.


Inhibition of thymidylate synthetase by showdomycin and its 5'-phosphate.


Purification of thymidylate synthetase from L. casei by affinity chromatography.


Changes in the membrane bound alkaline phosphatase of glucose and lactate grown vegetative cells of Bacillus subtilis SB15.


Four antigenic variants of blood group A glycolipid: examples of highly complex, branched chain glycolipid of animal cell membrane.


Acute responses of schizophrenic children to a sedative and a "stimulating" neuroleptic: a pharmacologic yardstick.


The therapeutic effects of lorazepam in psychotic patients treated with haloperidol: a double-blind study.


Thiothixene in the treatment of diseases of the senium.


A double-blind comparison of haloperidol (Haldol) and thioridazine (Mellaril) in outpatient children.


Therapy with cephaloridine in renal impairment.


A double-blind evaluation of Parafon Forte in the treatment of musculo-skeletal back conditions.


Sulfisoxazole-trimethoprim in gonococcal urethritis.


Alpha-methyltyrosine to prevent self-administration of morphine and amphetamine.


Possible treatment of aspiration pneumonia with bicarbonate solution.


Doxycycline blood levels in normal subjects after intravenous and oral administration.


Isoimmunization of man by recrystallized human insulin.


Long-term propranolol therapy in essential circulatory hyperkinesis.


Measurement of human ribosomal DNA in relation to a possible deficiency.


Avian Haemoproteidae. II. Haemoproteus enucleator sp. n. from the kingfisher, Ispidina picta (Boddaert).


Experimental transmission of an isolate of Nosema strigeoideae Hussey, 1971, to Fasciola hepatica.


Comparative antigenic consitution of Spriometra spp.


Precipitating antibodies in sheep infested with Psoroptes ovis (Acarina: Psoroptidae), the sheep scab mite.


Bone and cementum induction by dentin, demineralized in situ.


A note on Sporoza of undetermined taxonomic position in an armadillo and a heifer calf.


Ultrastructural localization of acid phosphatase in feeding Tokophrya infusionum.


Ciliary proteins and cytodifferentiation in Stentor coeruleus.


Mirex and Aroclor 1254: effect on and accumulation by Tetrahymena pyriformis strain W.


The effects of bacterial endotoxin on the infection of mice with Trypanosoma cruzi.


Infectivity and recovery of Tetrahymena pyriformis strain S from adult female cockroaches (Periplaneta americana).


Immunity to Babesia bigemina in experimentally infected cattle.


The molecular biology of Euglena gracilis. 8. Glycitols and monosaccharides.


Malignant lymphoma induced by partially purified Herpesvirus saimiri and recovery of infectious virus from tumorous lymph nodes.


Cellularity in different regions of adipose tissue in young men and women.


Lymphocyte surface immunoglobulins. Distribution and frequency in lymphoproliferative diseases.


Vestibulospinal projection in the toad.

Vestibulospinal projection in the toad.


Renal disease following prophylactic inoculation.


Origin of quick phases of nystagmus.

Origin of quick phases of nystagmus.


Vestibulo-ocular reflexes: effects of vestibular nuclear lesions.


Static oculomotor reactions, counter-rolling.


Vestibular influences on the vestibular and the somatosensory cortex.


Physiology and anatomy of the cortico-oculomotor mechanism.


Melanin hyperpigmentation in South African Bantu patients with toxic psychosis.


Running on two or on four legs: which consumes more energy? Disagreement exists over whether man's bipedal form of locomotion evolved as an economical means for covering long distances. There is also some disagreement about the energetic price man had to pay to free his hands. In an investigation of the relative energetic cost of bipedal and quadrupedal locomotion in primates, chimpanzees (Pan troglodytes) and capuchin monkeys (Cebus capucinus) were trained to run on a treadmill either on two or on four legs while their oxygen consumption was being measured. Both primates expend the same amount of energy whether running on two or on four legs. The relative energy cost of bipedal versus quadrupedal running should not be used in arguments about the evolution of bipedal locomotion in man.


The anatomy of infraclavicular subclavian vein catheterization and its complications.


Isolation of Aleutian mink disease virus by affinity chromatography. Affinity chromatography was used to isolate the Aleutian disease virus of mink. Dissociation of the immunoadsorbent-virus complex with 0.75 molar sodium chloride and then with a glycine-hydrochloride gradient released infective particles resembling picornaviruses. The elution profile suggests that two different types of virus-antibody complexes are formed, one dissociated by sodium chloride and another that requires glycine-hydrochloride in addition to sodium chloride for release of virus.


Apex cardiography.

Apex cardiography.


Increased osmotic resistance of human erythrocytes treated with saline extracts of the fava bean, lima bean, and isoimmune antibody of the ABO and Rh systems.


Species identification of invasive aspergillosis in man.


Determination of catechol-containing compounds in tissue samples by gas-liquid chromatography.


A contribution to the physical anthropology of the finns. Variations of the ABO, Rhesus, MN, P and Lewis blood group frequencies, PTC taste ability and colour blindness.


Particulate matter of particular interest.


Hypopituitarism secondary to hypothalamic insufficiency.


Positional preference of fatty acids in phospholipids of Bacillus cereus and its relation to growth temperature.


Ultraviolet inactivation of Euglena chloroplasts. I. Effect of light intensity of culture. Target numbers for ultraviolet (UV) inactivation of chloroplast replication in Euglena gracilis were about 45 when cells were grown in the dark and about 150 when grown in the light (700 or 1200 foot-candles [ft-cd]). Total cell DNA was about 25% greater in the light-grown cells.


Computer simulation of radial immunodiffusion. I. Selection of an algorithm for the diffusion process. Theories of diffusion with chemical reaction are reviewed as to their contributions toward developing an algorithm needed for computer simulation of immunodiffusion. The Spiers-Augustin moving sink and the Engelberg stationary sink theories show how the antibody-antigen reaction can be incorporated into boundary conditions of the free diffusion differential equations. For this, a stoichiometric precipitate was assumed and the location of precipitin lines could be predicted. The Hill simultaneous linear adsorption theory provides a mathematical device for including another special type of antibody-antigen reaction in antigen excess regions of the gel. It permits an explanation for the lowered antigen diffusion coefficient, observed in the Oudin arrangement of single linear diffusion, but does not enable prediction of the location of precipitin lines. The most promising mathematical approach for a general solution is implied in the Augustin alternating cycle theory. This assumes the immunodiffusion process can be evaluated by alternating computation cycles: free diffusion without chemical reaction and chemical reaction without diffusion. The algorithm for the free diffusion update cycle, extended to both linear and radial geometries, is given in detail since it was based on gross flow rather than more conventional expressions in terms of net flow. Limitations on the numerical integration process using this algorithm are illustrated for free diffusion from a cylindrical well.


A tumor-associated antigen in gastric cancer secretions.


Characterization of antiserum against myeloma cells.


Quantitative study of melanosome and mitochondrial populations in pigmented and amelanotic S-91 mouse melanomas.


The chemical modification of peptide antibiotics. I. The modification of gramicidin Dubos & the biological activity of the modified gramicidins.


Intestinal  -glycosidases:  -mannosidase &  -galactosidase from monkey small intestine.


-Fructofuranosidase of rumen protozoa.

-Fructofuranosidase of rumen protozoa.


Electron microscope localization of nuclear RNA's that shuttle between cytoplasm and nucleus and nuclear RNA's that do not. Nuclear transplantations and electron microscope autoradiographv (EMRA) were utilized in order to localize and characterize small nuclear RNA's (snRNA) in ameba nuclei. A class of nonmigrating low molecular weight nuclear RNA's is associated with the structureless region of the nucleoplasm but not with the nucleoli, nuclear helices, or chromatin Thus, the role of these RNA's in genetic regulation is questionable A class of migrating RNA's (presumed, but not directly shown, to be low molecular weight) that shuttles between nucleus and cytoplasm is also not associated with nucleoli or helices but some radioactivity is associated with the chromatin It may be, therefore, that the shuttling RNA's are in some way involved in genetic transcription or replication.


Membrane fusion in a model system. Mucocyst secretion in Tetrahymena. The freeze-fracture, freeze-etch technique can be employed to reveal new details of the process of fusion of two unit membranes For this study, mucocyst discharge in Tetrahymena pyriformis provides a model system with certain general implications The undischarged mature mucocyst is a saclike, membrane-bound, secretory vesicle containing crystalline material The organelle tip finds its way toward a special site, a rosette of 150 A diameter particles within the plasma membrane. To match this site, the mucocyst membrane forms an annulus of 110 A diameter particles, above whose inner edge the rosette particles sit. Discharge of some mucocysts is triggered by fixation. As discharge proceeds, the organelle becomes spherical and its content changes from crystalline to amorphous. The cytoplasm between the two matching membrane sites is squeezed away and the membranes fuse Steps in membrane reorganization can be reconstructed from changes in rosette appearance in the fracture faces. First, a depression in the rosette-the fusion pocket-forms. The rosette particles spread at the lip as the pocket deepens and enlarges from 60 to 200 nm. The annulus particles then become visible at the lip, indicating completed fusion of the A fracture faces of mucocyst and plasma membranes The remaining B faces of the two membranes have opposite polarities When the content of the mucocyst is released, the edges of these faces join so that the unit membrane runs uninterruptedly around the lip and into the pocket.


Observations on the effects of intrathecal papaverine in experimental vasospasm.


Fiber spectrum of the trigeminal sensory root of the baboon determined by electron microscopy.


Traumatic arachnoidal diverticulum associated with paraplegia. Case report.


Estimation of antibody protein in amniotic fluid to predict severity in rhesus haemolytic disease.


Long-term studies of residue retention and excretion by cows fed a polychlorinated biphenyl (Aroclor 1254).


Anencephaly and spina bifida: possible association with potato blight.


Analysis of polychlorinated biphenyls by gas chromatography and ultraviolet irradiation.


GABA and glutamate in different EEG stages of the penicillin focus.


Partial cell irradiation with a tunable organic dye laser.


Adaptive response of hominids to their environment as ascertained by fossil evidence.


Residues of polychlorinated biphenyls (PCB) and organochlorine insecticides in eggs from mute swan (Cygnus olor) and pochard (Aythya ferina).


Platelest counting: visual, electronic and fully automated optical methods.


Assay of platelet ATP and ADP by the luciferase method: some theoretical and practical aspects.


New metabolic products of Aspergillus flavus. I. Asperentin, its methyl ethers, and 5'-hydroxyasperentin.


Some aspects of magnesium and zinc metabolism in protein-calorie malnutrition.


New metabolic products of Aspergillus flavus. II. Asperflavin, anhydroasperflavin, and 5,7-dihydroxy-4-methylphthalide.


Studies on mono- and diiodohistidine. II. Congenital goitrous hypothyroidism with thyroglobulin defect and iodohistidine-rich iodoalbumin production. Butanol-insoluble iodinated compounds in the urine of patients with congenital goiters have been generally regarded as iodopeptides. Monoiodohistidine (MIH) and diiodohistidine (DIH) were identified from the urine of four patients with congenital goitrous hypothyroidism. From radioiodine studies, 40-70% of the urinary radioactivity was in the iodide-free fraction from which about 40% was identified as MIH and DIH by crystallizations to a constant specific activity. Iodotyrosines were simultaneously identified in the urine. However the presence of an iodotyrosine-deiodinase activity was demonstrated in the two removed goiters with a normal K(m) for MIT. In vivo iodotyrosine deiodination was normal for hypothyroid subjects. No thyroglobulin was identified in the thyroids from these patients. The major iodoprotein was iodoalbumin which, after in vivo labeling, contained 84-89% of the total soluble protein radioactivity. The thyroxine content of the goiter iodoalbumins and other iodoproteins was extremely low. Iodohistidines were identified in comparable proportions in the iodoalbumin and in the other iodoproteins isolated from each goiter. The average iodohistidine content of these proteins as crystallizable MIH and DIH was in the individual cases 15 and 4% of the in vivo incorporated radioiodine. DIH was identified in all iodoprotein fractions. The mean DIH/MIH ratios from the individual cases were 1.16 and 0.35. The corresponding DIT/MIT ratios were 3.19 and 1.45, respectively. The major consequence of this thyroglobulin defect is the iodination of inappropriate proteins (mainly albumin) resulting in low yields of thyroxine and high yields of iodohistidines. Iodohistidines from the goiter iodoproteins were not deiodinated and, at least for MIH, were quantitatively excreted in the urine of these patients. From the MIH iodoalbumin content and the MIH urinary excretion, goiter iodoalbumin turnover estimates were made and, although elevated, could not maintain a normal thyroxine secretion. The urinary excretion of iodohistidines easily demonstrated by column chromatography is offered as a test for detecting this variety of congenital goiter.


Primate biliary physiology. 8. The effect of phenobarbital upon bile salt synthesis and pool size, biliary lipid secretion, and bile composition. Phenobarbital, by inducing liver microsomal enzymes, may affect bile acid synthesis from cholesterol and thus alter the secretion of biliary lipids and the composition of bile. We, therefore, determined the effects of phenobarbital on bile flow, biliary lipid secretion, bile acid synthesis, and bile-acid pool size. Using an experimental preparation that allows controlled interruption of the enterohepatic circulation (1), we administered 5 mg/kg per day of phenobarbital to healthy Rhesus monkeys for 1-2 wk to achieve steady-state conditions. Three animals were studied with an intact enterohepatic circulation and three with a total bile fistula, each animal served as its own control. Total bile flow and secretion of bile salt, phospholipid, and cholesterol were measured every 24 h during steady-state conditions. Further, under conditions of an intact enterohepatic circulation bile-acid synthetic rate was measured in three animals and pool size estimated in two animals during both control and drug treatment periods. Phenobarbital at doses of 5 mg/kg per day increased bile flow 30-50% in all animals (P < 0.001). The increased bile flow resulted from both an increased "bile-salt independent fraction" and an increased bile-salt secretion rate. Phenobarbital significantly increased bile salt (P < 0.01) and phospholipid secretion (P < 0.05) by about 30% but cholesterol secretion was not significantly changed. Consequently, the concentration of cholesterol relative to bile salt and phospholipid was decreased (P < 0.001). Phenobarbital significantly enhanced the maximal rate of bile acid synthesis 25-30% in all three monkeys with total bile fistulas (P < 0.05) and also augmented bile acid synthesis and pool size in animals with intact enterohepatic circulations despite the fact that the rates of bile salt returning to the liver in these animals would have inhibited bile acid synthesis in control animals. Thus, phenobarbital not only increases the maximal rate of bile acid synthesis but also alters the normal control mechanisms by which bile salts returning to the liver inhibit bile salt synthesis. The fact that phenobarbital treatment results in increased synthesis of bile salt and unchanged secretion of cholesterol is consistant with the view that the drug augments conversion of hepatic cholesterol to bile salt. The resulting decrease in relative cholesterol content in bile may have therapeutic implications for cholesterol gallstone therapy.


The occurrence of single-stranded DNA in the serum of patients with systemic lupus erythematosus and other diseases. Single-stranded DNA (SDNA) occurs in high incidence and in greatest concentration in the sera of patients with systemic lupus erythematosus (SLE), where levels as high as 250 mug/ml were observed. SDNA appears to be an imunogen for anti-SDNA antibodies and forms complexes in vivo of both anti-SDNA-SDNA and anti-NDNA-SDNA types, which apparently play a role in the pathogenesis of the glomerulonephritis found in patients with SLE, SDNA is also found in high incidence but at lower levels in the sera of patients with rheumatoid arthritis. Lesser amounts of SDNA are found in several other diseases in which a low incidence of anti-SDNA antibodies is observed.


Modulation of pituitary responsiveness to thyrotropin-releasing hormone by triiodothyronine. The relative roles of triiodothyronine (T(3)) and thyroxine (T(4)) in modulating pituitary responsiveness to thyrotropin-releasing hormone (TRH) have been assessed. (a) 10 hyperthyroid patients with elevated serum T(2) and T(4) levels showed no pituitary response to TRH. After 2 wk of propylthiouracil therapy T(4) levels had fallen to normal in only five patients while T(2) levels were normal in all. Pituitary responsiveness to TRH returned in all patients with normal or high T(4) concentrations. (b) Patients with isolated elevations of serum T(3) (T(3) toxicosis) failed to respond to TRH. TRH responsiveness was restored when T(3) levels fell to normal after propylthiouracil therapy. (c) When pituitary responsiveness to TRH was tested 60 min after a single oral dose of 50 mug of T(3), which increased serum T(3) levels to slightly above the normal range, no rise in thyrotropin (TSH) was seen in six subjects. These findings indicate that T(3) elevations alone can rapidly inhibit pituitary responsiveness to TRH.


Degradation of thyroid hormones by phagocytosing human leukocytes. Thyroxine (T(4)) and triiodothyronine (T(9)) are rapidly degraded by a purified preparation of myeloperoxidase (MPO) and H(2)O(2) with the formation of iodide and material which remains at the origin on paper chromatography. Deiodination by MPO and H(2)O(2) occurs more readily at pH 7.0 than at pH 5.0 in contrast to iodination by this system which is known to occur more readily at pH 5.0 than at pH 7.0. Degradation is inhibited by azide, cyanide, ascorbic acid, and propylthiouracil. Methimazole stimulates deiodination by MPO and H(2)O(2) but inhibits this reaction when MPO is replaced by lactoperoxidase or horseradish peroxidase.Intact human leukocytes, in the resting state, degrade T(4) and T(3) slowly: degradation, however, is increased markedly during phagocytosis of preopsonized particles. Serum inhibits this reaction. T(3) can be detected as a minor product of T(4) degradation. Proteolytic digestion of the reaction products increases the recovery of monoiodotyrosine. The fixation of iodine in the cytoplasm of leukocytes which contain ingested bacteria was detected radioautographically. Chronic granulomatous disease leukocytes, which are deficient in H(2)O(2) formation, degrade T(4) and T(3) poorly during phagocytosis. MPO-deficient leukocytes degrade the thyroid hormones at a slower rate than do normal leukocytes although considerable degradation is still observed. Azide, cyanide, ascorbic acid, and propylthiouracil which inhibit certain peroxidasecatalyzed reactions inhibit degradation by normal leukocytes; however, inhibition is incomplete. Formation of iodinated origin material is inhibited to a greater degree by azide, cyanide, and propylthiouracil than is deiodination. Methimazole inhibits the formation of iodinated origin material by both normal and MPO-deficient leukocytes. However, deiodination by normal leukocytes is stimulated and that of MPO-deficient leukocytes is unaffected by methimazole. Hypoxia inhibits the degradation of T(4) and T(3) by untreated normal or MPO-deficient leukocytes and by normal leukocytes treated with azide or methimazole. These data suggest that both MPO-dependent and MPO-independent systems are involved in the degradation of T(4) and T(3) by phagocytosing leukocytes. The role of leukocytic degradation of T(4) and T(3) in thyroid hormone economy and in leukocytic microbicidal activity is considered.


Accelerated cellular uptake and metabolism of L-thyroxine during acute Salmonella typhimurium sepsis. The effects of acute Salmonella typhimurium sepsis on the kinetics of peripheral L-thyroxine (T(4)) distribution and metabolism and on serum total and free T(4) concentrations were studied in rhesus monkeys inoculated i.v. with either heat-killed or viable organisms. The rate of disappearance of labeled T(4) from serum was increased within 8 h after inoculation of monkeys with either heat-killed or viable Salmonella. The effects of the heat-killed organisms were transient and no longer evident by 16 h postinoculation. The monkeys inoculated with the viable Salmonella experienced a 2-3 day febrile, septic illness that was accompanied by an increase in the absolute rate of T(4) disposal. In the infected monkeys, serum total T(4) and endogenously labeled protein-bound iodine concentrations fell significantly during the period of acute sepsis and then rose during convalescence to values that exceeded the preinoculation values, suggesting that thyroidal secretion of hormone had increased in response to a primary depletion of the peripheral hormonal pool. Total cellular and hepatic uptakes of T(4) were enhanced by 4 h after inoculation of monkeys with either heat-killed or viable Salmonella, but the increase in total cellular uptake persisted for 24 h only in the monkeys inoculated with the viable organisms. These alterations in T(4) kinetics could neither be correlated with changes in the binding of T(4) in plasma nor attributed to an increase in vascular permeability. Moreover, they could not be ascribed to an in vitro product of bacterial growth, suggesting that the presence of the organisms themselves was required. An acceleration of T(4) disappearance was also observed during Escherichia coli and Diplococcus pucumoniae bacteremias. Our findings are consistent with a primary increase in the cellular uptake and metabolism of T(4) during bacterial sepsis, possibly related to phagocytic cell function in the host.


Prostaglandins, aspirin-like drugs and analgesia.


Parenteral nutrition with a solution of pure L-amino acids.


Aspects of the influence of psychic stress on angina pectoris.


Long-term methadone maintenance therapy: effects on liver function.


Australia antigen in rheumatoid arthritis.


Henneguya sp. (Sporozoa: Myxosporida) as a probable cause of death of Esox niger in Brome Lake, Québec.


Human anti-IgM iso-antibodies in subjects with selective IgA deficiency.


Carbonic anhydrase isozymes in Cavia porcellus, Cavia aperea and their hybrids.


Computerized helical scanning to determine the location of specific nuclides in the human body.


Treatment and evaluation of a plutonium-238 nitrate contaminated puncture wound. A two-year case history.


Serum cholinesterase and histopathology of the liver in severe protein calorie malnutrition.


Localization and growth pattern of the tergite Anlage of Drosophila.


The lack of influence of hemodilution perfusion on alterations in total body potassium.


Alterations in cerebral protein metabolism in the progeny of protein-calorie-deficient rats.


The effect of polychlorinated biphenyls on chicks. 3. Recovery.


Toxicity studies on polychlorinated biphenyls in the chick. 2. Biochemical effects and accumulations.


Failure of vitamin supplementation to alter the fatty liver syndrome caused by aflatoxin.


Ionizing radiation-initiated degradation of DNA in germinating spores.


Complement factors in multiple sclerosis.


Glucagon and diabetes mellitus.

Glucagon and diabetes mellitus.


H  chain fragment and monoclonal IgA in a lymphoproliferative disorder.


Hyperglucagonemia in diabetic ketoacidosis. Its prevalence and significance.


Pharmacology of a halogenated aralkylamine with anorectic properties.


Enzymically catalysed binding of amino acids to the protein cyst coat of the ciliate Colpoda steinii.


A modified procedure for fractionating histones. A method is described, which is capable of fractionating histones obtained from any animal source into five major groups. Although the method is based on procedures initially developed by E. W. Johns (1964), involving differential solubility in solutions of acids and of ethanol, it gives a cleaner separation and possesses the considerable advantage that the starting material is whole histone rather than a nucleoprotein preparation.


The activity of deoxyribonucleic acid polymerase in some species of algae. 1. The activities of DNA polymerase preparations from the algae Euglena gracilis, Chlamydomonas reinhardtii, Chlorella pyrenoidosa, Anabaena variabilis and Anacystis nidulans were measured. The blue-green algae Anabaena and Anacystis contain a 5-20-fold higher activity of the enzyme than do the green algae. DNA polymerases from the blue-green algae show a pH optimum of 9 and prefer a relatively low Mg(2+) concentration (1-3mm). DNA polymerases from the green algae, however, display a pH optimum between 7.5 and 8.5 and an optimum Mg(2+) concentration of 8mm. With all algae, a higher polymerase activity was obtained with denatured salmon sperm DNA as template than with native DNA. All four deoxyribonucleoside 5'-triphosphates must be present for full activity of the polymerases. 2. With one exception, the deoxyribonuclease activities in the preparations, measured under conditions of the DNA polymerase assay, are low compared with corresponding preparations from Escherichia coli. Chlamydomonas extracts contain a high deoxyribonuclease activity. 3. After purification on columns of DEAE-cellulose, the polymerase activity was linear over a wide range of protein concentrations, except for Chlamydomonas preparations, where the observed deviation from linearity was probably attributable to the high nuclease activity. 4. DNA polymerases from all these algae bind strongly to DNA-cellulose; 6-40-fold purifications of the enzyme were obtained by chromatography on columns of DNA-cellulose. 5. The partially purified polymerases of Euglena and Anacystis are heat-labile but become much more heat-stable when tested in the presence of DNA.


Different carnitine acyltransferases in calf liver.


Incorporation of L-(Me- 2 H 3 )methionine into isoprenoid quinones and related compounds by Euglena gracilis.


Multicentre, short-term therapeutic trial of disodium cromoglycate, with and without prednisone, in adults with asthma. A total of 117 asthmatic patients were studied in a double-blind, cross-over trial with half-periods of three weeks in which disodium cromoglycate (D.S.C.G.) was compared with an inactive preparation. D.S.C.G. improved spirometric measurements, at a very high level of statistical significance, more than did placebo. The mean advantage of D.S.C.G. was less than 10% after three weeks. Symptomatic improvement by D.S.C.G. was small though significant; it was not correlated with spirometric improvement. A history of chronic cough was the only clinical characteristic associated with a better-than-average spirometric response to D.S.C.G.


Metabolic disorders in diabetes.

Metabolic disorders in diabetes.


Parenteral fluid therapy.

Parenteral fluid therapy.


EEG paroxysms and echo-encephalography.

EEG paroxysms and echo-encephalography.


Learning and the concept of critical periods in infancy.


The treatment of epileptic children with carbamazepine. Follow-up studies of clinical course and EEG.


Observations on the fluorometric assay of histamine. Spermidine and spermine in brain.


Cephradine absorption and excretion in fasting and nonfasting volunteers.


The view of old age: no deposit, no return.


A team approach for care of long-term patients.


Characterization of infectious bursal disease.


Preparation of antilymphocytic globulin for human use.


The stress effect of rabbit antihuman lymphocyte globulin in rhesus monkeys.


A comparative study of antiglobulin antibodies and residual anti-D between recipients of intramuscular anti-D immunoglobulin and intravenous plasma anti-D.


Radioimmunoprecipitation assay for Australia antibody.


Detection of antibody to hepatitis-associated antigen in hemophilia patients and in voluntary blood donors.


Relation between blood groups and hepatitis associated antigen carrier state.


Hepatitis B antigen (HB Ag) and antibody (anti-HB Ag) in cold ethanol fractions of human plasma.


Circumstances limiting the development and verification of a comprehensive theory of craniofacial morphogenesis.


Antibodies to IGA in pregnancy and in recipient of Rh o (D) immune globulin (human).


A research perspective on craniofacial morphogenesis.


Storage of human platelets in liquid nitrogen isotopic studies.


Characterization of a structural antigen of rubella virus reacting by gel precipitation.


Purification of a soluble gel precipitating antigen of rubella virus and antibody responses to the purified antigen.


The problem of age estimation: a study using rhesus monkeys (Macaca mulatta).


Palatal growth rates in Macaca nemestrina and Papio cynocephalus.


Primary extragenital cutaneous gonorrhea.


Scraping for human scabies. A better method for positive preparations.


Long-term therapy of ischaemic heart disease.


Intestinal absorption of polychlorinated biphenyls in rats.


Characterization of the components of technically polychlorinated biphenyl mixtures. II.


Potential residue problem associated with low volume sprays on citrus in California.


Analysis of a chlorinated terphenyl (aroclor 5460) and its deposition in tissues of cod (Gadus morhua).


Inhalation tests in monkeys passively sensitized with human serum.


Interaction of 4-nitroquinoline 1-oxide with DNA in radiation-sensitive Bacillus subtilis.


Characterization of the DNA in Drosophila melanogaster. DNA has been quantitatively extracted from Drosophila melanogaster at various stages of embryonic development and analyzed by isopycnic centrifugation in CsCl and by fractionation on methylated albumin columns. The DNA is composed of three main classes of DNA, as defined by their buoyant density, rho, in CsCl: a bulk DNA, rho = 1.699 g cm(-3), and two satellite DNAs, rho = 1.685 g cm(-3) and rho = 1.669 g cm(-3). These three types of DNA persist throughout the development of the insect. In the unfertilized egg, 80% of the total DNA consists of the satellite DNAs; this amount decreases to 18% during the first three hours after fertilization and then remains constant through embryogenesis. There is a concomitant increase of the satellite DNA's with the bulk DNA after blastoderm formation.


Satellite DNAs in the embryos of various species of the genus Drosophila. A tentative evolutionary pattern has been found for two classes of the multiple satellite DNA's found in the genus Drosophila. The satellite DNA's from five Drosophila species (D. melanogaster, D. simulans, D. nasuta, D. virilis and D. hydei) were analyzed and found to fall into three arbitrary CsCl buoyant density classes: Class I, rho = 1.661-1.669 g cm(-3), DNA molecules composed of primarily dA and dT moieties; Class II, rho = 1.685 and rho = 1.692, DNA molecules of low GC content; and Class III, rho = 1.711, a DNA of high GC composition. The dAT satellite DNA's appear in all the species studied except D. hydei, the species of most recent evolutionary divergence, whereas the heavy satellite appears only in the two species of most recent divergence, D. virilis and D. hydei.


The effect of radiation-induced mutations on the fitness of Drosophila populations. The change in frequencies of D. melanogaster and D. simulans in competition experiments was used to measure the effect of radiation on the fitness of a population. A dose of 250 or 500 rads given to the males of highly inbred lines of D. simulans at the beginning of competition and every three weeks thereafter increased the relative frequency of the irradiated population. If the dose was increased to 1000 rads, the deleterious effects of radiation became too great a burden on the population, and the frequency of the irradiated population decreased. From these results it was concluded that below certain doses the introduction of radiation-induced mutations into a highly homozygous population would increase the fitness of the population.


A study of the genetic basis of the sexual dimorphism for wing length in Drosophila melanogaster. The genetic basis of a sexually dimorphic quantitative character in Drosophila melanogaster was investigated by means of two-way directional selection for increased and decreased differences between male and female wing length. The sex dimorphism (SD), defined as the mean wing length difference between the sexes, within families, provided the criterion for selection.-The two lines (High SD, Low SD) diverged rapidly during the 15 generations of selection, indicating the presence of extensive genetic variability for the genotype-sex interaction underlying the observed sexual dimorphism. There was evidence that genetic variability persisted in both lines when selection was relaxed. Most of the divergence between the two lines remained after 10 generations of relaxed selection.-The change in the level of sex dimorphism in the High line was due primarily to a decrease in male wing length; in the Low line most of the change in SD was the result of a decrease in female wing length. An overall reduction in wing length in both sexes in both lines is interpreted as an effect of inbreeding.-The distribution and nature of the genetic control underlying the SD characteristic of the two selection lines was investigated by chromosome substitution between selection lines using a marked inversion technique. The two lines differed by factors located on each of the three major chromosome pairs. Chromosome III had the greatest effect on the difference in SD level between lines, and showed an overall additive effect when present in homozygous versus heterozygous combination. Chromosome II had the least effect, with a significant dominance effect of the High II being evident when heterozygotes were compared with homozygotes. The effect of the X chromosome was intermediate. There was some evidence of interaction between non-homologous chromosomes.


A microbiological assay technique for hands.


Why wash walls in hospital isolation rooms?


Bacteriological techniques for testing carpets.


A method for the inclusion of Neisseria gonorrhoeae and Hemophilus influenzae in a mailed microbiology proficiency test program.


Use of an ergot preparation (hydergine) in the convalescent phase of stroke.


Pulmonary hypertension caused by minute amounts of endotoxin in calves.


The behavior of selected arthropod-borne viruses in agar gel diffusion systems.


Glycogen synthetase of human epidermis.

Glycogen synthetase of human epidermis.


Radiopharmacological studies on the cadmium poisoning.


Case for diagnosis.

Case for diagnosis.


A study of in vitro and in vivo resistance of the Taiwan gonococcus to graded penicillin dosages (with probenecid) with and without methicillin.


Minocycline and rifampin: proposed treatment regimen for the elimination of meningococci from the nasopharynges of healthy carriers.


Melioidosis: a serological survey in military personnel.


Dental care requirements of male Army recruits.


Menstrual abnormalities in the Wave recruit population.


Spontaneous splenic rupture in vivax malaria: case report.


Common psychological syndromes of the Army wife.


Drug damage and the gastrointestinal tract.


Disseminated intravascular coagulation.

Disseminated intravascular coagulation.


Radiation inactivation and recombination repair in Bacillus subtilis spores.


Human brucellosis in the Waikato: some aspects concerning laboratory diagnosis.


Creatinine transport in the maternal-fetal complex.


The Mersilene mesh gauze-hammock for severe urinary stress incontinence.


Behavioral effects of small oral doses of marihuana extract in chimpanzees.


Some biochemical correlates of inherited behavourial differences.


Some remarks about psychophysiological aspects of aggressive behaviour.


The effect of carbonic anhydrase inhibition on the canine in vivo H + -P CO2  relationship.


Immuno-diffusion study of the secretion of chick male oviducts.


Treatment of bladder carcinoma with 6 MeV linear accelerator.


Radiopharmaceutical dosimetry in pediatrics.


Measurement of body compartments in children: whole-body counting and other methods.


Evaluation of antihuman antilymphocyte serum with the rosette inhibition test using human and cynomolgus thymocytes.


The monkey skin graft model as an assay of human antilymphocyte globulin.


In vitro and in vivo properties of sheep antihuman thymocyte antibody isolated by a specific immunoadsorbent method.


Prevalence of serogroups and sulfonamide resistance of meningococci from the civilian population in the United States, 1964-1970.


Wood-pulp workers' disease: a hypersensitivity pneumonitis caused by Alternaria.


Preparation and characterization of 80 S ribosomes of ascites tumor cells active in polypeptide synthesis.


Activities and quantities of lysosomal enzymes during mammary tumor regression.


Radioimmunoassay for   1 -fetoprotein in the serum of rats. A radioimmunoassay (RIA) method for alpha 1-fetoprotein (AFP) in the serum of rats is reported.  The method is based on the preparation of purified and radiolabeled AFP, monospecific anti-AFP antiserum, and a modification of the coprecipitation-inhibition technique in 50% saturated ammonium sulfate.  A combination of immunochemical and physiocochemical methods are used in the purification of the AFP.  The purified AFP is virtually identical to the native, circulating AFP by immunological criteria and contains, at most, trace contamination with other materials.  The RIA has a sensitivity that allows 25 ng AFP/ml of serum to be detected with reproducibility.  This assay requires 20 mcl of serum and can be completed within a few hours.  It is concluded that this RIA, dependent upon the preparation of monospecific anti-AFP, radioiodination of highly purified rat AFP and a modified Farr procedure, is a sensitive and reproducible RIA.


Antibody activity in various classes of hedgehog immunoglobulins.


Evolutionary trends in plasma mercaptalbumin composition.


Metabolism of progesterone in the fetus and placenta of the rhesus monkey (Macaca mulatta).


Afrikander cattle congenital goiter: purification and partial identification of the complex iodoprotein pattern.


Sexual behavior and testosterone concentrations in the plasma of the rhesus monkey before and after castration.


Antagonism of insulin action on glucose metabolism in white fat cells by dexamethasone.


Metabolism of 14C-estradiol-17  injected intravenously into the pregnant baboon (Papio cynocephalus).


Culture of enzymatically dispersed pituitary cells: functional validation of a method.


Progesterone levels in the bonnet monkey (Macaca radiata) during the menstrual cycle and pregnancy.


Glomerular basement membrane-reactive antibodies in anti-lymphocyte sera: in vitro and in vivo characteristics.


Cell interaction by means of soluble factors (gamones) in conjugation of Blepharisma intermedium.


Preparation of rat liver cells. II. Effects of ions and chelators on tissue dispersion.


Electron probe analysis of refractive bodies in Amoeba proteus.


Albumin synthesis by isolated rat liver cells.


Insulin-like activity of proteases. I. Effect of pronase, a protease preparation from Streptomyces griseus, on glucose uptake by mouse diaphragm.


3 H-thymidine labeling patterns in polytene chromosomes of mitomycin-treated Drosophila melanogaster: evidence of continuous-type labelling as beginning of DNA replication.


Studies on bacteriolytic enzymes. II. Purification and some properties of two types of staphylolytic enzymes from Streptomyces griseus.


Chemical and immunological characterization of caprine erythrocyte glycolipids in comparison with porcine erythrocyte glycolipids.


Bitter peptides in the casein digests with bacterial proteinase. II. A bitter peptide consisting of tryptophan and leucine.


Meningococcal arthritis.

Meningococcal arthritis.


Cardiogenic shock in hypertrophic subaortic stenosis: case report.


Inhalation induced tolerance and physical dependence: the hazard of opiate suffused marihuana.


An unsuspected stab wound of the brain: case report.


Chromosomal aberrations in man due to low levels of ionizing radiation: a pilot study.


Venezuelan equine encephalomyelitis in Central America and Mexico.


Roots submerged to preserve alveolar bone: a case report.


The team approach to maxillofacial injuries.


Parenteral feeding.

Parenteral feeding.


Studies on intact and attrited human coronal dentin.


Glucose utilization of cardiac and skeletal muscle homogenates from fetal and adult rhesus monkeys.


Ureteral diverticulosis. Roentgenologic manifestation of ureteritis.


Biliary-duodenal dynamics in man. Cineradiographic and manometric studies following administration of drugs and hormones.


Determination of iron absorption from measurements of radioactive iron and a non-absorbed radioactive marker in a single faecal sample.


Treatment of Hodgkin's disease. Results obtained by individualized treatment using radiotherapy and cytostatics.


Vitamin B 12 -binding proteins in normal human blood plasma and serum.


Occurrence of aflatoxinogenic molds on "dry Cracower sausage".


Amylolytic activities of mesophilic and thermophilic varieties of Bacillus sp.


Anorexia: treatment.

Anorexia: treatment.


Psychophysical basis of coincidence mechanisms in the human visual system.


Anticonvulsant drugs. II. Clinical pharmacological and therapeutic aspects.


Antigenicity of rat follicular fluid.

Antigenicity of rat follicular fluid.


Comparison of measured and calculated high-LET nuclear recoil particle exposure on biosatellite 3.


Bremsstrahlung attenuation measurements in ilmenite loaded concretes.


An evaluation of micro-gel diffusion technique for grouping of beta hemolytic streptococci.


DDiscontinuities in the dorsal lateral geniculate nucleus corresponding to the optic disc: a comparative study.


The origins, course and distribution of the dorsal and intermediate acoustic striae in the rhesus monkey.


Thymidine triphosphate nucleotidohydrolase and deoxyuridylate hydroxymethylase induced by mutants of Bacillus subtilis bacteriophage SP82G. Assays on extracts of Bacillus subtilis infected by temperature-sensitive mutants of bacteriophage SP82G indicate that thymidine triphosphate nucleotidohydrolase may not be an essential SP82G enzyme and that deoxyuridylate hydroxymethylase is an essential enzyme coded for by SP82G gene 2.


Subcellular and intercellular aspects of tuberculous infection in reference to protection and sensitization.


Transcription during the development of bacteriophage phi 29: production of host- and phi 29-specific ribonucleic acid. The synthesis of ribonucleic acid (RNA) during development of the virulent Bacillus subtilis bacteriophage phi29 has been analyzed. Transcription of host deoxyribonucleic acid (DNA) continues at the preinfection rate throughout the latent period of viral growth. RNA-DNA hybridization was used to show that host messenger RNA synthesis continues late into the phage lytic cycle. Amino acid-labeling experiments show that this RNA is continuously used to produce protein. Ribosomal RNA production is not inhibited by phage infection. Small quantities of phage-specific RNA first appear between min 6 and 9 after infection. This RNA is made exclusively from one of the phi29 DNA strands. At 12 min postinfection, when phage DNA replication commences, large quantities of viral RNA start to be synthesized. This RNA appears to be transcribed from both strands of phi29 DNA. Studies with rifamycin and rifamycin-resistant host strains showed that the production of all phage phi29-specific RNA requires those components of the host RNA polymerase which are sensitive to this antibiotic. Thus, phage phi29 does not stop transcription of host DNA and may produce only one element for regulation of transcription of its own DNA. These findings may reflect the limited amount of genetic information carried by this phage.


Anti-cord factor antibodies.

Anti-cord factor antibodies.


Action of bacterial endotoxin on mitochondrial enzymic processes.


Effect of diphtherial cord factor and diphtherial toxin on mitochondrial oxidative phosphorylation.


Breast feeding and cot deaths.

Breast feeding and cot deaths.


Diphenylhydantoin.

Diphenylhydantoin.


The use of physical and animal models to assess bioavailability.


Isolation by covalent affinity chromatography of the penicillin-binding components from membranes of Bacillus subtilis. An affinity chromatography technique was developed to isolate the five penicillin-binding components present in Bacillus subtilis membranes. The proteins were solubilized by the detergent Nonidet P-40, bound covalently to penicillin-substituted Sepharose, and subsequently eluted from the matrix with neutral hydroxylamine, which cleaves the penicilloyl-enzyme bond. Penicillin binding-component V, the D-alanine carboxypeptidase, makes up 1% of the total membrane protein. A modification of the above procedure enabled this enzyme to be obtained from the membrane in pure form in a single step with 50% overall recovery of enzymatic activity.


Comparative toxicity study of 2,4,5,2',4',5'-hexachlorobiphenyl and a polychlorinated biphenyl mixture in rabbits.


Studies on poly I:C toxicity in experimental animals.


Phototoxicity and differential hepatotoxicity as biological indicators of nitrosamine activity.


Toxicologic implications of biotransformation by intestinal microflora.


'Cold' IgG autologous anti-LW. An immunological comparison with immune anti-LW.


The use of a range expander in automated anti-D quantitation.


Ankle and knee injuries at West Virginia University before and after astro turf.


Anaesthesia and the living cell: specificity of action.


Protein requirements of young cebus monkeys (Cebus albifrons and apella).


Effect of maternal malnutrition during pregnancy and lactation on hepatic protein metabolism in the infant rat: biochemical and ultrastructural studies.


Demonstration of tumor-associated antigens in human gynecologic malignancies.


Regeneration of endothelium in diseased and injured corneas.


Surgery, thio-tepa, and corticosteroid in the treatment of pterygium.


Alterations in the bactericidal ability of rabbit alveolar macrophages as a result of tumbling stress. Bacteriologic and electron microscopic studies support observations that the degradative phase of the phagocytic process in rabbit alveolar macrophages is altered by subjecting the animals to tumbling stress. Macrophages from control and stressed rabbits have similar ultrastructural features. In vitro incubation of macrophages from stressed animals with Pseudomonas aeruginosa showed that the process of bacterial ingestion was not impaired, nor were the numbers of bacteria ingested decreased as compared to control macrophages. However, the ability of macrophages from stressed animals to kill ingested bacteria was significantly decreased. Bacterial plate counts after 30 minutes incubation showed that approximately 70% of the ingested organisms had been killed by control macrophages, whereas there was practically no kill by macrophages from stressed animals even after 90 minutes. Electron microscopy demonstrated extensive degradation of Pseudomonas within phagocytic vacuoles of control macrophages. These changes consisted of cytoplasmic swelling, clumping of nuclear material and disruption of the bacterial cell wall. In contrast, bacterial degradation was not observed within macrophages from stressed animals at any of the intervals studied (up to 90 minutes after rotation). This form of stress thus appears to deprive the alveolar macrophages of the ability to destroy ingested microorganisms, a factor which may be important to the establishment of pulmonary infections.


Carboxyhemoglobin and the access to oxygen: an example of human counterevolution.


Effect of ovarian hormones on the uptake of  3 H-leucine by the castrated rat brain. An autoradiographic study.


Growth hormone covalently bound to Sepharose. I. Preparation and immunochemical properties.


The effects of progesterone on the patterns of testosterone and estradiol concentrations in the systemic plasma of the female rhesus monkey during the intermenstrual period.


Melanotropic-lipolytic peptides in various regions of bovine, simian and human brains and in simian and human cerebrospinal fluids.


Bovine prolactin, growth hormone, thyroxine and corticoid response to thyrotropin releasing hormone.


Radioimmunoassay for follicle stimulating hormone in the rhesus monkey using an anti-human FSH serum and rat FSH131I.


Synergism of testosterone and estradiol in the development and function of the magnum from the immature chicken (Gallus domesticus) oviduct.


Species differences in luteinizing hormone as inferred from slope variations in a radioligand receptor assay.


Unclassified spondylo-epiphyseal dysplasia. Report of two cases.


Effect of synthetic human, porcine, and salmon calcitonin on calcium and phosphorus in immobilized children.


Effect of temperature on survivability of bacteria in blood in vitro in presence of anticoagulants.


Production of vitamin B 12  by Streptomyces spp.


The phakomatoses.

The phakomatoses.


Regulation of microtubules in Tetrahymena. II. Relation between turnover of microtubule proteins and microtubule dissociation and assembly during oral replacement. Experiments are reported which were designed to test for induced synthesis of microtubule proteins associated with the rapid proliferation of basal bodies and associated intracytoplasmic microtubules which occurs during oral replacement in Tetrahymena. None was found. Instead, it is shown that these structures can be formed with de novo synthesis of as little as 6% of their microtubule proteins. It is suggested that basal body proliferation may be controlled by synthesis of morphogenetic regulator proteins.


Structures linking the myonemes, endoplasmic reticulum, and surface membranes in the contractile ciliate Vorticella. An electron microscope investigation of the interface between the myonemes of Vorticella convallaria and their associated endoplasmic reticulum (ER) has revealed structures of a complex morphology linking these two organelles. These structures are named "linkage complexes". Each complex contains a spindle-shaped midpiece which lies in a groove of the ER membrane. Microfilaments splay out from the tips of the midpiece and may come in contact with the inner alveolar sac membrane. Three to six raillike structures lie on each side of the midpiece and parallel it. The ER membrane appears to pass through the sides of the rails. In the lumen of the ER these rails are associated with a meshwork of filaments. A cradle of five rods lies within the groove under the midpiece. The ER membrane also passes through these rods which contact the same meshwork. In the scopular region and in the stalk the microfilaments from the midpiece form a bundle which passes into the lumen of modified basal bodies. These basal bodies are connected to the alveolar sac which, in the stalk, passes as a flattened tube along its length. The parts of the dissociated linkage complex are scattered throughout the spasmoneme of the stalk along membranes of the intraspasmonemal tubules. Thus, both stalk and body contractile bundles have linkage complexes that link their associated membrane systems to the microfibrils and, in turn, connect this membrane-microfibrillar interface to the pellicular membranes. The arrangement of the linkage complex suggests an involvement in the control of the transport of calcium ions between ER and microfibrils, and possibly the transfer of a message from the surface membranes to the sites of calcium release to trigger myonemal contraction.


Nitroglycerin sublingual tablets. I. Stability of conventional tablets.


Isolation and characterization of mitochondrial DNA from Drosophila melanogaster. Mitochondrial DNA (MtDNA) with a neutral buoyant density of 1.681 g/cm(3) has been isolated from unfertilized eggs of Drosophila melanogaster. This DNA is a circular molecule with an average length of 5.3 microm; it reassociates with a low C(0)t(1/2) after denaturation, and in alkaline isopycnic centrifugation it separates into strands differing in density by 0.005 g/cm(3). MtDNA isolated from purified mitochondria of unfertilized eggs or from total larval DNA melts with three distinct thermal transitions. The three melting temperature values suggest that the molecule may have three regions differing in average base composition. DNA isolated from unfertilized eggs of D. melanogaster contains approximately equal amounts of MtDNA and another DNA with a buoyant density of 1.697 g/cm(3), slightly less dense than main peak DNA. The possibility that the heavier DNA fraction consists of amplified ribosomal DNA was excluded by hybridization experiments, but otherwise nothing is known of its origin or function.


Comparison of ampicillin and hetacillin pharmacokinetics in man.


Effects of vasoconstrictive drugs on the placental circulation of the Rhesus monkey. A preliminary report.


Congress of the South African Nutrition Society. Opening address.


Epilepsy among brain-injured veterans 26 to 31 years following the injury.


Correlative studies of dynamics and pathology in whip-lash and head injuries.


Blue-green algae: why they become dominant. The injection of carbon dioxide and the addition of nitrogen and phosphorus to a lake population dominated by blue-green algae results in a rapid shift to dominance by green algae. The basis for the change and its implications are discussed.


Counting fetal erythrocytes as a diagnostic aid in perinatal death and morbidity.


Clinical patterns of osteomyelitis due to gram-negative bacteria.


Tissue pressure and histology of normal and inflamed tooth pulps in macaque monkeys.


Dentofacial remodelling produced by intermaxillary forces in Macaca mulatta.


Binding of ethidium bromide to double-stranded ribonucleic acid.


Preliminary study of colony type stability of Neisseria gonorrhoeae in liquid culture.


Neuronal loss and gliosis in the hippocampus following repetitive epileptic seizures induced in adolescent baboons by allylglycine.


Treatment of gonococcal urethritis with a single dose of doxycycline monohydrate.


The continuous arterial infusion of 5-fluorouracil as a therapeutic adjunct in the treatment of advanced carcinoma of the bladder and prostate. A preliminary report.


The identification and metabolic origin of 2-furoylglycine and 2,5-furandicarboxylic acid in human urine.


Orthogonal immunodiffusion and immunoelectrophoresis of serum proteins.


The assay of pyruvate kinase activity in blood cells.


Detection of hepatitis-associated antigen and antibody in concentrated sera from hepatitis patients.


Environmental contamination and control facilities.


Hepatitis B antigen and antibody in blood donors: an epidemiologic study.


Aerobic and anaerobic microbial flora in the urinary tract of geriatric patients during long-term care.


Bactericidal antibody after colonization with Neisseria meningitidis.


Antigenic analysis of echoviruses 1 and 8.


An indirect fluorescent-antibody technique for study of uncomplicated gonorrhea. II. Selection and characterization of the strain of Neisseria gonorrhoeae used as antigen.


An indirect fluorescent-antibody technique for study of uncomplicated gonorrhea. I. Methodology.


Secretory IgA antibodies in the urethral exudate of men with uncomplicated urethritis due to Neisseria gonorrhoeae.


Synthesis of 1,2-dihydro-1-(2-deoxy- -D-erythro-pentofuranosyl)-2-oxopyrazine 4-oxide, a potent analog of deoxyuridine.


A difference-differential model in population dynamics.


Future drug research--drugs of the future.


Comparative study of tobramycin and gentamicin with special reference to anti-pseudomonas activity.


Distribution of carbonic anhydrase in mammalian salivary glands.