Genetics of the ST serotype system in Tetrahymena pyriformis, syngen 1. Genetic analyses using lines of Tetrahymena pyroformis manifesting different serotypes indicate that the St serotypes are governed by alleles at a single genetic locus. These alleles are termed St(A) and St(C). The St locus is not closely linked to any of the other well-studied loci examined. Differentiation in St(A)/St(C) heterozygotes follows a pattern very similar to that observed with lines heterozygous at the other loci. Initially both alleles are expressed, but as the synclone divides, lines develop that manifest one allele or the other but not both. The time of differentiation is very early in the clonal life cycle, and the output ratio is eccentric. The pattern of development of the St locus places it in a category with the mating type and H serotype loci.


Extrachromosomal element delta in Drosophila melanogaster. VII. Relation to fertility in a second-chromosome line. The ID(b)-45 chromosome line usually carries an appreciable amount of delta b, but it is not susceptible to the killing action of this delta. The fertility of this line was examined when it carried various amounts of delta b. More than one third of the Cy/ID(b)-45 males and females tested became sterile when they carried cytoplasm of the Cy/Pm stock which is assumed to carry no delta b. The number of progeny is smaller when flies of this line are raised at 25 degrees C than at 28 degrees C at which temperature the multiplication of delta is accelerated. The progeny number was appreciably reduced when the flies were raised at 18 degrees C at which temperature the multiplication of delta is suppressed. This line could not be maintained at that temperature, since both males and females became sterile. Thus, the conclusion may be drawn that the presence of an appreciable amount of delta b is necessary for the gametogenesis of the Cy/ID(b)-45 flies.


Extrachromosomal element delta in Drosophila melanogaster. 8. Inseparable association with sensitive second chromosome. The presence of delta is always accompanied by a sensitive second chromosome in Drosophila melanogaster or vice versa. The separability of delta and the chromosome line was investigated in experiments designed to eliminate delta or to suppress its multiplication for a number of generations. Cy/S(b)-5 males which transmit a minute amount of delta b (retained by chromosome S(b), kills S(b)/S(b) and S(b)/S(r) zygotes) to their progeny were backcrossed for 61 generations to Cy/Pm females which carried no delta. After backcrossing 465 Cy/S(b)-5 males were individually examined for retention of delta, and all were found to retain delta b in its latent state. A homozygous strain for the S(r)-20B chromosome which retained a minute amount of delta r (retained by chromosome S(r), kills S(r)/S(r) zygotes) was obtained. 1,252 Cy/S(r)-20B females derived from the homozygous strains at the 21st generation were tested individually for delta retention. All females tested were found to retain delta r in its latent state. The delta retention in S(b)-5 chromosome lines isolated from S(r)-Cy/S(b)-5 heterozygous strains which carried delta r but not delta b was examined. The descendant Cy/S(b)-5 lines from the heterozygous strains had accumulated their specific delta, delta b. These findings are consistent with those obtained in an earlier study, and lead to the conclusion that delta is associated inseparably with each specific sensitive chromosome.


Factors affecting recognition and disjunction of chromosomes at distributive pairing in female Drosophila melanogaster. I. Total length vs. arm length. The behavior of a compound metacentric fourth chromosome (see PDF) has been examined to determine whether arm length or total length is the basis for recognition in distributive pairing. Recognition was judged by the frequency with which the (see PDF) nondisjoined from a series of X duplications (Dp), ranging in size from </= 0.3 to > 4 times the size of a single fourth chromosome. Dp, (see PDF) nondisjunction was measured in the absence and in the presence of a competitor, a compound metacentric X. In both situations, total length and not arm length, was found to confer the characteristic recognition property to the (see PDF). A comparison of Dp, (see PDF) nondisjunction curves for both the noncompetitive and competitive situations with analogous Dp, 4 curves previously obtained, show the Dp, (see PDF) curves to be similar in shape to those obtained earlier but displaced one unit to the right, corresponding precisely to the difference in size between the (see PDF) and the 4. Rules governing chromosome recognition for acrocentrics were found completely applicable to metacentrics; disjunctive behavior of metacentrics differed from that of acrocentrics in that two arms conferred on a chromosome the capacity to act as the intermediate of a trivalent when size no longer warranted this attribute. This capacity, itself, is size-dependent.


Factors affecting recognition and disjunction of chromosomes at distributive pairing in female Drosophila melanogaster. II. The effect of a second arm. The behavior of heterozygously inverted X chromosomes that were members of the distributive pool at least 70% of the time was studied when the other pool members were either two free 4's or one compound 4. The X's were structurally modified by additions or deletions of heterochromatin, so that the two homologues differed in both size and configuration or in size alone. In the noncompetitive situation, with two free 4's, recognition between the X's remained high despite the modifications, and primary X nondisjunction was low. In the competitive situation, with the compound 4, distributive nondisjunction of the X's increased approximately two orders of magnitude, and trivalent formation was indicated. Disjunction from the trivalent varied with X size and configuration. When both X's were acrocentric, the smaller X directed the larger X and the very small (see PDF) to the same pole; when the larger X carried a second arm, it assumed the directing role; when the size ratio of the smaller, one-armed X to the larger, two-armed X became less than approximately 5/9, the smaller X again directed the other two.


The effects of heterozygosity changes on viability in Drosophila melanogaster. The viability effects of chromosomes from an old and from a new laboratory strain of D. melanogaster were studied in eight factorial combinations and at two heterozygosity levels. The combinations were so constructed that heterozygosity level could be varied in the third chromosomes of the carriers of a homozygous lethal marker, in the third chromosomes of their wild-type segregants, and in the genetic backgrounds of both. Excluding the effect of the marker and the exceptional outcomes of two of the combinations, and taking into account both large and small deviations from theoretical expectation, the following summary is given as the simplest consistent explanation of the results: 1) If total heterozygosities of two segregant types tend toward equality their viabilities tend toward equality also, whether background heterozygosity is high or low; if background heterozygosities is higher the tendency toward equality is slightly greater. 2) If total heterozygosity of two segregant types are unequal the less heterozygous type has the lower viability; the difference is more pronounced when background heterozygosity is low, less when it is high. 3) Differences between segregant viabilities are correlated with differences between the total heterozygosities of the two segregants; genetic background is effective to the extent, and only to the extent, that it contributes to the magnitude of this difference. This in turn appears to underlie, at least partly, the expression of a pronounced interchromosomal epistasis. Thus in this study viability is seen to depend upon both the quantity and distribution of heterozygosity, not only among the chromosomes of an individual but among the individuals of a given combination as well.


A study of haematological values in newborns.


Carbonic anhydrase B concentration of erythrocytes in chronic renal failure.


Studies on candicidin biogenesis.

Studies on candicidin biogenesis.


Antimetabolites produced by microorganisms. V. L-2-amino-4-methoxy-trans-3-butenoic acid.


Studies on new antibiotic lividomycins. V. In vitro and in vivo antimicrobial activity of lividomycin A.


A new dipeptide antibiotic from Streptomyces collinus, Lindenbein.


Effect of tunicamycin on microorganisms: morphological changes and degradation of RNA and DNA induced by tunicamycin.


Behavioral effects of infectious diseases: Phlebotomus fever in man.


Ischemic anoxia and cerebral serotonin levels.


Total intravenous alimentation: foreword.


The effects of protein hydrolysate-monosaccharide infusion on low-birth-weight infants.


Total parenteral nutrition in premature infants.


Total intravenous alimentation in low-birth-weight infants: a preliminary report.


Hyperammonemia accompanying parenteral nutrition in newborn infants.


Intravenous alimentation.

Intravenous alimentation.


Hyperammonemia resulting from intravenous alimentation using a mixture of synthetic l-amino acids: a preliminary report.


The salvage of babies and children with congenital heart disease.


Treatment of severe angina pectoris.

Treatment of severe angina pectoris.


Management of patients with chronic obstructive jaundice.


Nutrition in the post-traumatic period.

Nutrition in the post-traumatic period.


The effect of newer tubercle bacillus digestion and decontamination procedures on fungi causing pulmonary diseases.


Balanced intravenous nutrition in pediatric surgery.


Long-term complete intravenous nutrition in man.


The nutrition of patients with extensive burns.


Long-term parenteral nutrition following massive intestinal resection.


Factors affecting the nitrogen balance in chronic uremic patients receiving essential amino acids intravenously or by mouth.


The comparative metabolism of carbohydrates administered intravenously.


Clinical aspects of body fluid osmolality.


Carcinoma within lower esophageal (epiphrenic) diverticulum.


Intermittent chemotherapy in pulmonary tuberculosis--current aspects.


Choledochoduodenostomy and sphincteroplasty. For calculous biliary tract disease.


Species differences in the metabolism of a tricyclic psychotropic agent, SQ 11,290- 14 C.


Toxicity of propylene glycol 1,2-dinitrate in experimental animals.


Open assessment of disodium cromoglycate of Nigerian asthmatics.


Longitudinal study of the morbidity of diarrheal and respiratory infections in malnourished children.


Tissue culture studies of common Nigerian arboviruses: propagation in different tissue culture systems.


A double-tracer test of the oral absorption of  57 Co- and  58 Co-vitamin B 12  using a whole-body monitor: clinical experience and technical factors.


A rapid tissue technique for the field assessment of protein-caloric malnutrition.


Anatomical and functional specializations of the human gluteus maximus.


Prenatal growth of long bones in rhesus and squirrel monkeys (Macaca mulatta and Saimiri sciureus).


Craniofacial growth in juvenile Macaca mulatta: a cephalometric study.


Tyrosinase positive oculocutaneous albinism among the Zuni and the Brandywine triracial isolate: biochemical and clinical characteristics and fertility.


Blood groups and H-Le a  salivary secretion of Brazilian Cayapo Indians.


Electron microscopic localization of macromolecules on membrane surfaces.


Discussion paper: bacterial transport mechanisms as studied in cytoplasmic membrane vesicles.


Are inorganic cations esential for the stability of biological membranes?


The present dilemma in national health care.


Two case reports: successful treatment of carious exposures with Ledermix.


Comparison of policies of sensitivity testing of tubercle bacilli in Hong Kong.


Freezing and storage in liquid nitrogen of axenically and monoxenically cultivated Tetrahymena pyriformis.


Genetical and maternal determinants of the activity and preening behaviour of Drosophila melanogaster reared in different environments.


Competition in Drosophila. II. Homozygous lines.


New Simbu viruses: antigenic analysis by complement fixation and immunodiffusion tests.


Absence of laccase from yellow-spored mutants of Aspergillus nidulans.


The microbial flora of the gut of the pouch-young and the pouch of a marsupial, Setonix brachyurus.


Migration inhibition of peripheral leucocytes in human schistosomiasis.


Human heat labile opsonins: evidence for their mediation via the alternate pathway of complement activation.


A starfish hemolymph factor which activates vertebrate complement in the presence of cobra venom factor.


Studies on the relative synthesis and distribution of IgA and IgG1 in various tissues and body fluids of the cow.


Structure and biologic functions of human IgD. I. The presence of immunoglobulin D in human cord sera.


Identification of "J" chain in a homogeneous canine IgA immunoglobulin.


Determination of relative affinity and heterogeneity of mouse anti-DNP antibodies by a plaque-inhibition technique.


Reaginic antibody formation in the mouse. I. Antibody-mediated suppression of reaginic antibody formation.


Live-weights and body measurements of olive baboons (Papio anubis) in the Laikipia district of Kenya.


Twinning in the new world monkey, Cebus apella.


Heterocyclic O-substituted hydroxylamines. Antiinflammatory activity and possible inhibition of histamine biosynthesis.


Synthetic thyrotropin-releasing factor analogs. 3. Effect of replacement or modification of histidine residue on biological activity.


Occurrence and distribution of aromatic L-amino acid (L-DOPA) decarboxylase in the human brain.


Basic ethers of 2-anilinobenzothiazoles and 2-anilinobenzoxazoles as potential antidepressants.


Raised intracranial pressure and cerebral blood flow. I. Cisterna magna infusion in primates. Changes in cerebral blood flow during incremental increases of intracranial pressure produced by infusion of fluid into the cisterna magna were studied in anaesthetized baboons. Cerebral blood flow remained constant at intracranial pressure levels up to approximately 50 mm Hg. At intracranial pressure levels between 50-96 mm Hg a marked increase in cerebral blood flow occurred, associated with the development of systemic hypertension and changes in cerebrovascular resistance. Further increases of intracranial pressure led to a progressive fall in cerebral blood flow. Prior section of the cervical cord prevented both the increase in cerebral blood flow and the systemic hypertension. Alteration of cerebral perfusion pressure by bleeding during the hyperaemia in a further group of animals suggested that autoregulation was at least partially preserved during this phase. After maximum hyperaemia had occurred, however, autoregulation appeared to be lost. The clinical implications of these findings are discussed.


Nerve conduction during Wallerian degeneration in the baloon. Conduction in the lateral popliteal nerve of the baboon was studied during the course of Wallerian degeneration. Six nerves were examined. In each case the muscle response to nerve stimulation and the ascending nerve action potential were recorded daily until the nerve became inexcitable. The muscle response to nerve stimulation disappeared after four to five days, but ascending nerve action potentials could be recorded for a further two to three days. There was no change in maximal motor conduction velocity or in distal latency until the muscle response to nerve stimulation was severely reduced in amplitude. At this stage there was a consistent increase in distal latency, sometimes associated with a mild reduction in maximal motor velocity in the leg. There was no change in the velocity of ascending nerve action potentials. Histological studies confirmed the presence of degeneration in the terminal parts of the intramuscular nerve fibres at a time when the proximal parts of the same fibres were relatively normal.


Experimental siderosis and liver injury in the rhesus monkey.


Parenteral feeding--a note of caution.

Parenteral feeding--a note of caution.


Alterations in blood biochemical tests in progressive protein malnutrition.


Proper packaging and storage of nitroglycerin tablets.


The prevention of exercise induced asthma.


Behçet's syndrome, Crohn's disease and toxic megacolon.


Evidence that phentolamine acts centrally to increase the contractile force of the heart.


Souble  -aminolevulinic acid synthetase of rat liver. I. Some properties of the partially purified enzyme.


The proteolytic enzymes of the K-1 strain of Streptomyces griseus obtained from a commercial preparation (Pronase). I. Purification of four serine endopeptidases.


The proteolytic enzymes of the K-1 strain of Streptomyces griseus obtained from a commercial preparation (Pronase). II. The activity of a serine enzyme in 6 M guanidinium chloride.


Protocatechuate 3,4-dioxygenase. 3. An oxygenated form of enzyme as reaction intermediate.


Studies on the biology of the periodontium of marmosets. XIV. The effect of experimentally produced osteomyelitis on the marmoset periodontium.


Absorption, distribution, metabolism, and excretion of naproxen in various laboratory animals and human subjects.


Electrokinetic studies of bacteria. II. Effect of cetyltrimethylammonium bromide on Streptococcus faecalis.


Detergents.

Detergents.


Effects of orally administered thiamine tetrahydrofurfuryl disulfide on foetal development of rabbits and monkeys.


Independence of glucagon receptors and glucagon inactivation in liver cell membranes.


Sprained ankle.

Sprained ankle.


Further studies on antihuman thymocyte IgG. Antibody specificities, opsonin activity correlated to immunosuppression, lymphopenia, and hyporesponsiveness to horse IgG in primates.


Immunochemical characterization of a monoclonal  G4,lambda human antibody to factor IX.


Immunologic rebound after cessation of long-term chemotherapy in acute leukemia.


Local chemotherapy in bladder cancer.

Local chemotherapy in bladder cancer.


A further case of ureteric diverticulum--congenital or acquired?


Vasomotor projections of the nucleus fastigii to the medulla.


Regional localization of histamine and histidine in the brain of the rhesus monkey.


On the function of lymphocytes in malignant effusions.


Comparing costs of hospital and community care.


The trouble with malpractice.

The trouble with malpractice.


Surgery, in brief.

Surgery, in brief.


Patients' councils speed rehabilitation.


Rat  M immunoglobulin: isolation and some biological characteristics.


Structural studies on a mouse myeloma  M immunoglobin.


Activity of brain stem neurons during eye movements of alert monkeys.


Lateralization performance of squirrel monkey (Samiri sciureus) to binaural click signals.


Air-borne B. subtilis enzymes in the detergent industry.


Responses of squirrel monkey (Samiri sciureus) medial geniculate units to binaural click stimuli.


Activity of superior colliculus in behaving monkey. I. Visual receptive fields of single neurons.


Activity of superior colliculus in behaving monkey. II. Effect of attention on neuronal responses.


Activity of superior colliculus in behaving monkey. 3. Cells discharging before eye movements.


Activity of superior colliculus in behaving monkey. IV. Effects of lesions on eye movements.


The incidence of alpha 1 -antitrypsin defiencies in an industrial population.


Function of the alveolar macrophage in immunity.


Selective vestibular ablation in monkeys by laser irradiation.


Why ECFs and hospitals should cooperate, and why they don't.


Habituation: electrical changes in the visual system.


Tactile discrimination learning after selective prefrontal ablations in monkeys (Macaca mulatta).


Visual discrimination learning after selective prefrontal ablations in monkeys (Macaca mulatta).


Non-reversal shifts after selective prefrontal ablations in monkeys (Macaca mulatta).


A selective spatial deficit in monkeys after transection of the fornix.


Experimentally induced pulpitis.

Experimentally induced pulpitis.


Continued evaluation of the effects of a sclerosing agent on odontogenesis.


Interferon messenger RNA: translation in heterologous cells. A viral inhibitor with the characteristics of mouse interferon is produced by avian and simian cells preincubated with RNA extracted from interferon-producing mouse cells. Similarly, RNA extracted from interferon-producing monkey cells induces a monkey interferon-like substance in avian cells and also in a line of simian cells, VERO, which normally lacks the capacity to produce its own interferon. In both cases, the RNA effect is inhibited by treatment of the receptor cells by cycloheximide, but not by actinomycin D. We conclude that interferon messenger RNA has been translated in the receptor cells. Thus, the production of interferon in heterologous cells can be used as a sensitive assay of interferon messenger RNA.


A brain protein unique to the olfactory bulb. Soluble extracts from several regions of the mouse brain manifested regionally specific protein band patterns on polyacrylamide gel electrophoresis. In particular, one protein band appeared to occur uniquely in the olfactory bulb extracts where it was a quantitatively significant constituent of the soluble protein extract. This protein was purified to homogeneity by ammonium sulfate precipitation, DEAE-cellulose column chromatography, and polyacrylamide gel electrophoresis. The purified protein has a minimum molecular weight of about 20,000 by gel electrophoresis in the presence of sodium dodecyl sulfate. Precipitating antiserum prepared against this protein reacted only with the purified protein or with extracts of olfactory bulb, and not with extracts of other mouse tissues or brain regions. Quantitative immunoprecipitation assays indicate that this specific protein represents 1% of the total soluble protein in the mouse olfactory bulb. Soluble extracts of olfactory bulbs from several rodent species gave reactions of identity by agar gel diffusion with the antiserum to the mouse protein. It is suggested that this protein is an example of selective genetic expression within the central nervous system.


Bacteriophage Q  replicase contains the protein biosynthesis elongation factors EF Tu and EF Ts. The enzyme, Qbeta replicase, responsible for the replication of the RNA of Escherichia coli pahge Qbeta, is composed of four nonidentical subunits, three of which, I, III, and IV, are coded for by the bacterial genome, while subunit II is phage-specific. SUBUNIT IV IS SHOWN TO BE IDENTICAL TO THE PROTEIN SYNTHESIS ELONGATION FACTOR EF TS BY THE FOLLOWING CRITERIA: coelectrophoresis on polyacrylamide gels in sodium dodecyl sulfate and in urea buffers, identity of the first seven amino acids at the amino-terminus, precipitation of sub-unit IV by anti-EF T-factor serum, and stimulation of EF Tu-GDP exchange by subunit IV. Subunit III is shown to be identical to the protein synthesis elongation factor EF Tu by the following criteria: coelectrophoresis on sodium dodecyl sulfate gels, precipitation of EF Tu by anti-Qbeta replicase serum, binding of guanine nucleotides, and binding of phenylalanyl-tRNA. In addition, Qbeta replicase activity can be reconstituted from subunits I and II with EF Tu and EF Ts.


Ribosomal RNA synthesis in vitro: a protein-DNA complex from Bacillus subtilis active in initiation of transcription. A protein-DNA transcription complex, isolated from logarithmic-phase cells of Bacillus subtilis, is active in the initiation of new rounds of RNA synthesis in vitro. Transcription directed by the endogenous DNA of the complex is sensitive to inhibitors of initiation. In addition, the effect of addition of a competing template indicates that RNA polymerase in the complex is bound to endogenous DNA in a dissociable, preinitiation state. Hybridization-competition analysis of the RNA product obtained from the in vitro reaction indicates that it is greatly enriched for ribosomal RNA sequences. This initiating complex should be useful for the study of regulation of expression of ribosomal RNA genes.


Circadian systems: longevity as a function of circadian resonance in Drosophila melanogaster. Drosophila melanogaster, which had been reared under standard conditions (25 degrees and a 24-hr light/dark cycle involving 12 hr of light) were exposed, on the first day of adult life, to four environments (all at 25 degrees ) as follows: (i) a 24-hr day consisting of 12 hr light and 12 hr dark; (ii) a 21-hr day (10.5 hr light, 10.5 hr dark); (iii) a 27-hr day (13.5 hr light, 13.5 hr dark); and (iv) constant light. The experiment was repeated four times. In all four experiments the flies on a 24-hr day lived significantly longer than the flies in the other environments. This result, comparable to other observations on plants, indicates that eukaryotic systems as oscillators perform most effectively when they are driven close to their natural "circadian" frequency.


Early extracellular events in infection of competent Bacillus subtilis by DNA of bacteriophage SP82G. Analysis, by the recovery of specific genetic "markers," of the effects of DNase I, physical shear, and temperature shock on DNA-cell complexes demonstrates that sequential attachment of both ends of bacteriophage SP82G DNA to Bacillus subtilis precedes entry of the DNA molecule into the cell, and that each attachment is end-and time-specific. The first attachment involves an initial reversible phase, followed by irreversible binding. After a latent period, the second end then attaches to the cell. Entry of the molecule begins immediately after binding of the second end has occurred, and entry is complete within 3 min. The polarity of entry, as judged by attainment of resistance to DNase I, is the reverse of that observed in normal phage injection.


Treatment of perforated duodenal diverticula. Report of a case.


Histochemical study of enzymes of optic nerve of monkey and rat.


Erythrocyte passage into Schlemm's canal.


Dominant goniodysgenesis with late congenital glaucoma. A re-examination of Berg's pedigree.


Sterility of the aqueous humor following cataract surgery.


Ultracentrifugal studies in capillary cells. II. Sedimentation equilibrium molecular weight determinations.


Difference spectral method for determination of native G-actin concentration.


A new histaminase assay.

A new histaminase assay.


Parenteral hyperalimentation and its practical applications.


Continuous production of extracellular protease by Bacillus subtilis in a two-stage fermentor.


Circulating antibodies in dermatophytosis.


Effects of lithium chloride on normal and neoplastic cells in vitro.


Effects of natural toad serum on heterologous vertebrate cells.


Ability of luteinizing hormone (LH) to acutely increase serum progesterone levels during the secretory phase of the rhesus menstrual cycle. The acute response (increase in serum progesterone) of rhesus monkeys to a single intravenous injection of luteinizing hormone (LH), follicle-stimulating hormone (FSH), or prolactin was examined.  Blood samples were obtained at 0, 20, 40, 60, and 120 minutes posttreatment with gonadotropins on the morning of Day 6 or 21 of the cycle.  Neither FSH nor LH induced an increase in progesterone when administered on Day 6.  LH increased serum progesterone at all dose levels (50, 250, and 500 mcg) during the luteal phase of the cycle.  Values for LH-treated animals were significantly greater than saline controls (P  .05), were positively correlated with dose (.632), and the regression was significant (p  .01).  The mean effect of 1000 mcg of FSH suggests the ability of exogenous FSH to evoke a slight increase in progesterone secretion.  Removal of the LH contaminant in FSH by incubation with LH antiserum did not influence the ability of FSH to increase progesterone levels, however, administration of LH antiserum just prior to giving FSH blocked the previously noted progesterone response to FSH.  60 IU of prolactin was without influence in both phases of the cycle.  It is concluded that FSH induces changes in progesterone levels by either affecting the receptor site for LH or causing endogenous release of LH.


The  -glycerophosphate in Drosophila melanogaster. II. Genetic aspects. Seven alleles of the alpha-Glycerophosphate dehydrogenase-1 (alphaGpdh-1) locus of Drosophila melanogaster have been described. These include two naturally occurring electrophoretic variants, one EMS-induced electrophoretic variant, and four EMS-induced "null" or "zero" mutants. With the electrophoretic variants, the locus was mapped to II-20.5 +/- 2.5. A complementation matrix was prepared utilizing the null mutants. Three of the four mutants and a deletion of the locus (Grell 1967) exhibit dosage dependency. The dosage independent mutant exhibits complementation with two of the other null alleles. Flies genetically deficient in alpha-glycerophosphate dehydrogenase are fertile, but their relative viability is severely diminished. Such flies also lose the ability to sustain flight, an observation consistent with the enzyme's function in energy production. The levels of mitochondrial alpha-glycerophosphate oxidase, measured in flies genetically deficient in the cytoplasmic enzyme, were normal.


The anatomy and function of a segment of the X chromosome of Drosophila melanogaster. An average size chromomere of the polytene X chromosome of Drosophila melanogaster contains enough DNA in each haploid equivalent strand to code for 30 genes, each 1,000 nucleotides long. We have attempted to learn about the organization of chromosomes by asking how many functional units can be localized within a chromomere. This was done by 1) recovery of mutants representative of every cistron in the 3A2-3C2 region; 2) the characterization of the function of each mutant type and grouping by complementation tests; 3) the determination of the genetic and cytological position of each cistron by recombination and deletion mapping. The data clearly show one functional group per chromomere. It is postulated that a chromomere is one cistron within which much of the DNA is regulatory in function.


Phosphoglucomutase and carbonic anhydrase in West Malaysian aborigines.


Segmental aneuploidy and the genetic gross structure of the Drosophila genome. By combining elements of two Y-autosome translocations with displaced autosomal breakpoints, it is possible to produce zygotes heterozygous for a deficiency for the region between the breakpoints, and also, as a complementary product, zygotes carrying a duplication for precisely the same region. A set of Y-autosome translocations with appropriately positioned breakpoints, therefore, can in principle be used to generate a non-overlapping set of deficiencies and duplications for the entire autosomal complement.-Using this method, we have succeeded in examining segmental aneuploids for 85% of chromosomes 2 and 3 in order to assess the effects of aneuploidy and to determine the number and location of dosage-sensitive loci in the Drosophila genome (Figure 5). Combining our data with previously reported results on the synthesis of Drosophila aneuploids (see Lindsley and Grell 1968), the following generalities emerge.-1. The X chromosome contains no triplo-lethal loci, few or no haplo-lethal loci, at least seven Minute loci, one hyperploid-sensitive locus, and one locus that is both triplo-abnormal and haplo-abnormal. 2. Chromosome 2 contains no triplo-lethal loci, few or no haplo-lethal loci, at least 17 Minute loci, and at least four other haplo-abnormal loci. 3. Chromosome 3 contains one triplo-lethal locus that is also haplo-lethal, few or no other haplo-lethal loci, at least 16 Minute loci, and at least six other haplo-abnormal loci. 4. Chromosome 4 contains no triplo-lethal loci, no haplo-lethal loci, one Minute locus, and no other haplo-abnormal loci.-Thus, the Drosophila genome contains 57 loci, aneuploidy for which leads to a recognizable effect on the organism: one of these is triplo-lethal and haplo-lethal, one is triplo-abnormal and haplo-abnormal, one is hyperploid-sensitive, ten are haplo-abnormal, 41 are Minutes, and three are either haplo-lethals or Minutes. Because of the paucity of aneuploid-lethal loci, it may be concluded that the deleterious effects of aneuploidy are mostly the consequence of the additive effects of genes that are slightly sensitive to abnormal dosage. Moreover, except for the single triplo-lethal locus, the effects of hyperploidy are much less pronounced than those of the corresponding hypoploidy.


On the estimation of gene frequencies through gene counting.


Mode of cell migration to the superficial layers of fetal monkey neocortex.


A stereotaxic atlas of the brainstem for Macaca mulatta in the sitting position.


Metabolism of T4 bacteriophage ghost-infected cells. II. Do ghosts cause a generalized permeability change? Ghosts appear to alter the barrier function of the cell membrane, allowing the release of phosphorylated sugars which normally cannot pass through the cell membrane, whereas phage do not. No increased influx of normally impermeable compounds is observed in the presence of ghosts, indicating that the loss of membrane function after ghost infection cannot be attributed to a generalized breakdown of the permeability barrier.


Rapid demonstration of minor carboanhydrase isoenzyme fractions in human erythrocytes.


Not so "specific" dynamic action.

Not so "specific" dynamic action.


Sympathetic adrenergic innervation of permanent teeth in the monkey (Macaca irus).


Pancreaticoduodenectomy for periampullary tumours.


Retinal receptor orientation and the role of fiber optics in vision.


A study in Hong Kong to evaluate the role of pretreatment susceptibility tests in the selection of regiments of chemotherapy for pulmonary tuberculosis. Hong Kong Tuberculosis Treatment Services-British Medical Research Council Investigation.


Quantitative determination of several pleural fluid proteins.


Changes in lung volumes and airway resistance after exercise in asthmatic subjects.


A protein-bound phosphorylated product as an intermediate in the biosynthesis of O-methyl glycerol by Mycobacterium tuberculosis.


Absence of carbonic anhydrase in red cell membranes.


Bone morphogenetic protein and proteinase in the guinea pig.


Comparative anatomy of the talus in relation to idiopathic clubfoot.


Result study of ruptures of lateral ligaments of the ankle.


Strontium content of mammalian bone, diet and excreta.


The amount of ribosomal RNA genes in Tetrahymena pyriformis in different physiological states.


Fine structure of the M fibres in Stentor before and after shortening.


Outbreak of hand-foot-and-mouth disease among Indian and Eskimo children in a hospital.


Pseudomonas aeruginosa exotoxin: effect on cell cultures.


Primary isolation of TRIC organisms in HeLa 229 cells treated with DEAE-dextran.


Product of T4 gene 12.

Product of T4 gene 12.


Gaboon viper venom and its effects.

Gaboon viper venom and its effects.


The effect of a sucrose-enriched diet on the metabolism of intravenously administered fructose in baboons.


[Types and virulence of Mycobacterium tuberculosis in peripheral tuberculous lymphadenitis].


-Hydroxy fatty acids in sphingolipids of Tetrahymena.


Fatty acid methyl and ethyl esters in Tetrahymena pyriformis.


Positional distribution of fatty acids in phospholipids from Bacillus subtilis.


Lifetime of the excited state in vivo. I. Chlorophyll a in algae, at room and at liquid nitrogen temperatures; rate constants of radiationless deactivation and trapping. Using a mode-locked laser (lambda, 632.8 nm), fluorescence decay of chlorophyll (Chl) a in the green alga Chlorella pyrenoidosa, the red alga Porphyridium cruentum, and the blue-green alga Anacystis nidulans was measured by the phase-shift method under conditions when photosynthesis was not operative (3-(3,4-dichlorophenyl)-1,1-dimethylurea [DCMU] poisoning, or cooling to 77 degrees K). In the presence of 10(-5) M DCMU, the lifetime of Chl a fluorescence (tau) at room temperature is about 1.7 nsec in Chlorella, 1.0 nsec in Porphyridium, and 0.7 nsec in Anacystis. At 77 degrees K, tau is 1.4 nsec (for fluorescence at about 685 nm, F-685) and 2.3 nsec (for F-730) in Chlorella, 0.9 nsec (F-685) and 1.2 nsec (F-730) in Porphyridium, and 0.8 nsec (F-685 and F-730) in Anacystis. From the above measurement, and the assumption that tau(0) (the intrinsic fluorescence lifetime) for Chl a in all three algae is 15.2 nsec, we have calculated the rate constants of radiationless transition (that includes energy transfer to weakly fluorescent system I) processes competing with fluorescence at room temperature to be about 5 x 10(8) sec(-1) in Chlorella, 9 x 10(8) sec(-1) in Porphyridium, and 13 x 10(8) sec(-1) in Anacystis. At 77 degrees K, this rate constant for Chl a that fluoresces at 685 nm remains, in the first approximation, the same as at room temperature. From the tau data, the rate constant for the trapping of excitation energy is calculated to be about 1.2 x 10(9) sec(-1) for Chlorella, 2 x 10(9) sec(-1) for Porphyridium, and 2 x 10(9) sec(-1) for Anacystis. The efficiency of trapping is calculated to be about 66% (Chlorella), 68% (Porphyridium), and 60% (Anacystis). (It is recognized that variations in the above values are to be expected if algae grown under different conditions are used for experimentation.) The maximum quantum yield of Chl a fluorescence for system II (lambda, 632.8 nm), calculated from tau measurements, is about 10% in Chlorella, 6-7% in Porhyridium, and 5% in Anacystis under conditions when photosynthesis is not operative; the values at 77 degrees K appear to be very close to those with DCMU added at room temperature. ø for F-730 at 77 degrees K, however, is somewhat higher than for F-685. The predicted quantum yields of fluorescence for Chl a in intact cells (both systems I and II) at low intensities of 632.8 nm light are about 2-3, 1-2, and 1% for Chlorella, Porphyridium, and Anacystis, respectively.


Lifetime of the excited state in vivo. II. Bacteriochlorophyll in photosynthetic bacteria at room temperature. Lifetime of the excited state (tau) of bacteriochlorophyll (BChl) in photosynthetic bacteria, measured with a mode-locked argon laser (oscillating at 488 nm; mode locked at 56 MHz) as light source, ranged from 0.3 to 2.5 nsec. These tau values are reported with a precision of +/-0.1 nsec. The value of tau at high exciting light intensity (I) was two to three times that at low intensity. For young cultures of green bacterium Chloropseudomonas ethylicum, tau ranged from 0.5 (low I) to 1.0 nsec (high I); for those of the purple bacterium Rhodospirillum rubrum, from 0.4 (low I) to 1.0 nsec (high I); and for those of the BChl b-containing Rhodopseudomonas viridis, from 1.0 (low I) to 2.5 nsec (high I). These data provide information regarding the efficiencies of the photochemical process in these bacteria. Quantum yield (ø) of BChl fluorescence, calculated from ø = tau/tau(0) (where tau(0) is the intrinsic lifetime of fluorescence), ranges from 2-6% at low intensities to 6-14% at high intensities.


Chromatographic and biological aspects of polychlorinated biphenyls.


Surface topography and electron probe analysis of carbonic anhydrase-containing cells in the turtle bladder mucosa.


Viruses from South American monkeys: ultrastructural studies.


The comparative anatomy of the interpeduncular nucleus in the brain of the rat, cat and monkey.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 31-1972.


Who needs radiologists?

Who needs radiologists?


Competition between different scrapie agents in mice.


Preference for ethanol in the rhesus monkey following chronic infusion of ethanol into the cerebral ventricles.


Production of Borna virus in tissue culture.


Endocrine effects of grouping and dominance rank in squirrel monkeys.


Rheophoresis: a sensitive immunodiffusion method for detection of hepatitis associated antigen.


Biological properties of Norwalk agent of acute infectious nonbacterial gastroenteritis.


Stimulation by synthetic thyrotropin-releasing hormone of glucose oxidation in porcine and rat pituitary slices.


The effect of leukocytic endogenous mediator (LEM) on the tissue distribution of zinc and iron.


In vitro tests of thyroid function in active thyroglobulin immunity.


Effect of implantation of anti-LH serum into median eminence on rat pituitary and serum LH.


Radioprotective effects of dimethyl sulfoxide in Drosophila melanogaster.


On the radiolysis of aqueous solutions of nickel (II) ethylenediamine tetraacetate.


Some aspects of the cellular and humoral immune response to tumour antigens.


The site of the adaptation shown in figural after effects.


Selective chromatic adaptation in primate photoreceptors.


Oral enteric bacterial vaccines. Report of a WHO scientific group.


Characteristics of arginases from plant, ureotelic and uricotelic organisms.


Foreign bodies of the biliary tract: report of two patients and a review of the literature.


Autonomic reinnervation of cardiac transplants: further observations in dogs and rhesus monkeys.


Some methodological modifications of the technicon "SMA 12-60 AutoAnalyzer" system.


Ultrastructure of the macronuclear anlage in Stylonychia mytilus.


Antimitotic agents and macronuclear division of ciliates. V. Cytokinesis of Tetrahymena synchronized by deuterium oxide.


Colcemid action on the division schedule of synchronized Tetrahymena.


"Nucleoli" in micronucleus of Tetrahymena.


Study on the influence of the rhesus system on skin graft survival.


What is your diagnosis? Ossifying fibroma.


Experimental subacute sclerosing panencephalitis in the hamster: correlation of age with chronic inclusion-cell encephalitis.


Epidemic diphtheria and skin infections in Trinidad.


Pseudomonas aeruginosa exotoxin: effect on cellular and mitochondrial respiration.


Eight years of surveillance of patients hospitalized with hepatitis: interpretation of data in light of epidemic parenteral drug abuse and availability of testing for hepatitis-associated antigen.


Concentration of chorionic gonadotrophin and chorionic somatomammotrophin in maternal serum, amniotic fluid and cord blood serum at term.


Speculations about gingival repair.

Speculations about gingival repair.


Intravenous alimentation in the neonate and infant.


The "effective thyroxine ratio" in the assessment of thyroid function.


Peripheral self-stimulation as a reward in fish, reptile and mammal.


Role of visual cuing in force discrimination by monkeys.


"Clinical gonorrhea" in the female: laboratory findings.


Vector ecology. Report of a WHO scientific group.


Body weight decreases in some proton exposed primates.


Changes in arterial subfractions with aging and atherosclerosis.


Transport of L-leucine and D-leucine into pancreatic  -cells with reference to the mechanisms of amino acid-induced insulin release.


Purification and properties of hemagglutinin from Pseudomonas aeruginosa and its reaction with human blood cells.


Antibody to lactate dehydrogenase. V. Use as a carrier for introducing diphtheria toxin into mouse tumor cells.


Trypsin treatment of adipocytes: effect on sensitivity to insulin.


Reciprocal relationship between the production of adrenal damage by 7,12-dimethylbenz(a)anthracene in rats and the induction of liver damage by various treatments. Further investigations into the mechanism by which CCl(4) administration to Sprague-Dawley rats protects them against the adrenocorticolytic action of dimethylbenz(a)anthracene (DMBA) are reported. The results show that CCl(4) must be given shortly before DMBA to achieve the best protection and that treatments given after DMBA are ineffective. It was established that the hepatotoxicity of CCl(4) in these experiments was related reciprocally to the adrenocorticolytic effect of DMBA.Protection with butter yellow (DAB) was achieved only when sufficient time elapses for drug metabolism to be stimulated in the liver. Butter yellow given after DMBA has no protective effect but the prior exposure of the rats to DMBA potentiates the hepatotoxic effects of DAB.Partial hepatectomy gives protection when performed 1 day before DMBA; shorter intervals give no protection. Some protection can be achieved with resection 6 or 24 hours after DMBA.


Catecholamine metabolite excretion following intraventricular injection of 6-OH-dopamine.


The effects of manganese on the rabbit anterior mesenteric-portal vein.


On the question of vagal tone in monkeys (Macaca mulatta).


The heart rate in monkeys (baboons and macaques) in different physiological states recorded by radiotelemetry.


Composition of squirrel monkey milk.

Composition of squirrel monkey milk.


XX male presbytis entellus? A retrospective study.


Effects of ovarian hormones upon the sexual skin and mounting behaviour in the free-ranging chacma baboon (Papio ursinus).


Contribution to the ecology of Mandrillus sphinx Linnaeus 1758 of Rio Muni (Republic of Equatorial Guinea).


Chromosome segregation influenced by two alleles of the meiotic mutant c(3)G in Drosophila melanogaster.


Differences in synaptic affinity of chromosome arms of Drosophila melanogaster revealed by differential sensitivity to translocation heterozygosity.


Sperm preference in Drosophila melanogaster.


Dynamics of polymorphisms. I. Selection components in an experimental population of Drosophila melanogaster.


Microtubule-arms and propulsion of food particles inside a large feeding organelle in the ciliate Phascolodon vorticella.


Sympathetic and parasympathetic control of heart rate in the dog, baboon and man.


Pilot studies of a porous implant in dentistry and oral surgery.


Actinomycosis of the tongue.

Actinomycosis of the tongue.


Venous tissue replanted on roots of teeth in monkeys.


Immobilisation, compensation and field shaping in megavolt therapy.


Anomalous parietal sutures and the bipartite parietal bone.


Congenital defects of cattle: nature, cause, and effect.


Total body opacification in the adult.

Total body opacification in the adult.


Cumulative dose from diagnostic irradiation.


Research strategies in alcoholism.

Research strategies in alcoholism.


Estradiol-receptors of the rat uterus: interaction of the cytoplasmic estrogen-receptor with DNA in vitro.


Properties of Euglena gracilis cytoplasmic ribosomes in salt.


Selective isolation of ovomucoid using an insolubilised trypsin derivative.


Isolation of a heme protein antigen from Ascaris suum.


Circular dichroism of kappa- and lambda type Bence-Jones proteins at various pH values and temperatures.


Infectivity and distribution of infectious bursal disease virus in the chicken. Persistence of the virus and lesions.


The fine structure of Leucocytozoon simondi. V. The oocyst.


Serum   1 -antitrypsin levels during the ovarian cycle.


Evidence for the existence of mammalian acetoacetate decarboxylase: with special reference to human blood serum.


Urinary amino acid output in children with Hurler's syndrome.


Artificial substrates in the assay of acid glycosidases.


Complement levels in acute infectious hepatitis and serum hepatitis.


Effects of thyrotrophin-releasing hormone on plasma levels of TSH, FSH, LH and GH in anorexia nervosa.


The reduction of antigenicity of heterologous antilymphocyte serum with acid Taka-protease.


The effects of inversions and the C(3)G mutation on intragenic recombination in Drosophila.


Geriatric care in Italy.

Geriatric care in Italy.


The nature of carbenicillin resistance in Pseudomonas aeruginosa.


The production of penicillins in soils and seeds by penicillium chrysogenum and the role of penicillin  -lactamase in the ecology of soil bacillus.


Amino sugars in the wall of Pseudomonas fluorescens.


Rapid localization of bacterial surface antigens by whole-mount immunoperoxidase technique.


The assay of ecdysones and juvenile hormones on Drosophila imaginal disks in vitro.


The lethal effect of long-chain fatty acids on mycobacteria.


Complications following intravenous administration of solutions containing xylitol.


Lactic acidosis and osmotic diuresis produced by xylitol infusion.


More disadvantages of bottle feeding.

More disadvantages of bottle feeding.


Private and public health-care costs.

Private and public health-care costs.


Kerosene poisoning in primates.

Kerosene poisoning in primates.


The effect of antibiotic stress on protein synthesis in the establishment of lysogeny of Plectonema boryanum.


Comparisons of togaviruses: Sindbis virus (group A) and Kunjin virus (group B).


Polypeptide synthesis in influenza virus-infected cells.


Influence of amphetamines on animal behaviour: stereotypy, functional impairment and possible animal-human correlations.


Virus-associated DNase: endonuclease in a polyhedral cytoplasmic deoxyvirus.


Psychoses and the punding and choreiform syndromes in addiction to central stimulant drugs.


Evidence that particle size heterogeneity is the cause of centrifugal heterogeneity in tobacco streak virus.


Intramuscular lipid store utilization by contracting dog skeletal muscle in situ.


Psychopharmacology of amphetamines.

Psychopharmacology of amphetamines.


Clinical significance of LATS and TSH in the blood.


Response of large and small coronary arteries to nitroglycerin, NaNO 2 , and adenosine.


Chemotherapy of melanoma of the extremities by perfusion: fourteen years clinical experience.


Pharmacokinetics of drug action.

Pharmacokinetics of drug action.


Infections with Myocbacterium marinum.

Infections with Myocbacterium marinum.


Effect of antibiotics on the lipases of Corynebacterium acnes in vitro.


Correlation between various proteins of bovine snout epidermis.


The treatment of oral cancer.

The treatment of oral cancer.


Sacrococcygeal teratomas in children.

Sacrococcygeal teratomas in children.


Report on national surveys of cytologic facilities.


Tumor angiogenesis.

Tumor angiogenesis.


Malignant melanoma: current concepts of lymph node dissection.


A spirit of adventure: a personal tribute to Wendell G. Scott, M.D. (1905-1972).


Unproven methods of cancer management. Laetrile.


Classics in oncology. Charles Brenton Huggins.


Biology of cancer and the cancer cell: normal and abnormal regulation of cell reproduction.


Studies on prostatic cancer. I. The effect of castration, of estrogen and androgen injection on serum phosphatases in metastatic carcinoma of the prostate.


An electron microscopic study of central and peripheral nodes of Ranvier.


A comparative evaluation of Transgrow and Stuart's transport medium in the diagnosis of gonococcal infection.


The number of villi in rat's jejunum and ileum: effect of normal growth, partial enterectomy, and tube feeding.


Degeneration of the peripheral visual pathway in captive monkeys.


Immunochemical study of hypoxanthine-dehydrogenase in different organs of the chick.


Cell lines derived from late embryonic stages of Drosophila melanogaster.


Parathyroid hormone in foetal and adult sheep: the effect of hypocalcaemia.


The metabolism of starch, glucose, amino acids, purines, pyrimidines and bacteria by the rumen ciliate Entodinium simplex.


Sporulation in Bacillus subtilis. Genetic analysis of oligosporogenous mutants.


Lysis of streptococci by an extracellular lysin produced by competent group H streptococcus strain CHALLIS.


Sporulation in Bacillus subtilis. Characterization of oligosporogenous mutants and comparison of their phenotypes with those of asporogenous mutants.


Pulpal response of monkeys to modifications of a bisphenol A composite resin and cement.


Dentin and bone formation in scorbutic monkeys.


Absence of compensatory renal hypertrophy in baboons. 1. The weights of kidneys of fifteen adolescent and forty-six adult male baboons were estimated. In contrast with what has been described in other mammals, in the majority of baboons (thirty-nine out of fifty-seven) the left kidney was heavier than the right.2. The medulla/cortex ratio was of the order of 1.8. The maximum urinary concentration, after 36 hr of food and water deprivation, averaged 1050 +/- 80 m-osmole/kg H(2)O.3. Unilateral nephrectomy was performed in twelve baboons with body weight ranging from 5 to 14 kg. In six animals, the left kidney was removed and the right kidney in the others.4. In two baboons, the left kidney was transplanted to the left iliac artery and vein, the ureters being left intact. After a few weeks, the right kidney was taken out, and the animals were kept under observation, one for a further 3 months and the other for a further 4 months.5. In none of the animals, whether after simple unilateral nephrectomy or after unilateral nephrectomy following transplant of one kidney, did the renoprival kidney show any significant enlargement.6. After unilateral nephrectomy, the health of the animals remained excellent, all the baboons increasing in body weight. The ability to concentrate urine by the renoprival kidney was not impaired.7. In contrast with what has been described in mice and rats, in unilaterally nephrectomized baboons no significant changes in the concentration of either RNA or DNA in renal cortex and medulla of the contralateral kidney were observed.8. After unilateral nephrectomy in baboons, there was a marked increase of oxygen uptake in slices from the cortex, but not from the medulla. In contrast with what had been observed in rats where the enhanced rate of oxygen uptake returned to pre-operative values, in baboons no decrease was observed, the rate of oxygen uptake remaining high during the whole period of observation.9. In the limit of time of observation (maximum 4 months) there was no evidence that unilateral nephrectomy resulted in compensatory hypertrophy of the contralateral kidney.


Mycobacterial adjuvant. I. Purification of an adjuvant-active component from wax D(H 37 Ra strain) by preparative thin-layer chromatography.


Modern concepts in the treatment of cholera.


Babesia pantherae sp.nov., a piroplasm of the leopard (Panthera pardus).


Antibacterial activity of the combination ampicillin-kanamycin against gram-negative bacteria.


The procaine esterase activity of serum from different mammalian species.


Serum immunoglobulins in leprosy patients with different spectra of clinical manifestations.


Stability studies of human fibrinopeptide A as measured by radioimmunoassay.


Immunologic studies of acetylated encephalitogenic myelin basic protein.


The effect of 6-azauridine upon subacute sclerosing panencephalitis virus in tissue cultures.


Studies of the C3 shunt activation in cobra venom induced lysis of unsensitized erythrocytes.


Spread of scrapie to sheep and goats by oral dosing with foetal membranes from scrapie-affected sheep.


Myxosporidiosis in Coho salmon.

Myxosporidiosis in Coho salmon.


Characterisation of antibodies active in the Rose Bengal plate test.


Canine pneumonia associated with mycoplasma infection.


An evaluation of quantitative serum immunoglobulin determinations in clinical practice.


Precipitating antigens of hemagglutinating encephalomyelitis virus demonstrated by agar gel immunodiffusion.


An autoantibody with U-specificity in a patient with myasthenia gravis.


Water-soluble I blood group substance in the secretions of i adults.


Reversible disaggregation by NaF of membrane-bound polyribosomes of mouse myeloma cells in tissue culture.


Regulation of yolk protein synthesis in amphibian liver. I. Induction of lipovitellin synthesis by estrogen.


Is ceruloplasmin a physiological ferroxidase?


Survival of nonsporulating microorganisms in laboratory and natural conditions.


Surgical management of carcinoma of the oral cavity.


Regulation of RNA synthesis.

Regulation of RNA synthesis.


The evolution of the hallucial tarsometatarsal joint in the Anthropoidea.


Serum cholinesterase activities and inhibition profiles of nonhuman primates.


The role of basicranial synchondroses in flexure processes and ontogenetic development of the skull base.


An analytical review of the effects of non-hydrogen-bonding anesthetics on memory processing.


Tuberculous peritonitis in pregnancy.

Tuberculous peritonitis in pregnancy.


Lactic acidosis with phenformin therapy. Nine cases of severe acidosis occurring in patients with maturity onset diabetes who were receiving phenformin therapy were reviewed. All had evidence of renal disease. Lactic acidosis was diagnosed by elevated blood lactate levels in seven patients, and by the clinical manifestations and laboratory information in the other two. Contributing factors are discussed, as well as means of treatment and prevention. Although the specific etiology of renal disease is not emphasized in this paper, unrecognized progressive deterioration of renal function is implicated in the etiology of lactic acidosis.


Subclinical Brucella infection in man.

Subclinical Brucella infection in man. Much subclinical infection with Brucella abortus affects the dairy farming community, slaughterhouse workers, and veterinary surgeons. High titres of antibody to B. abortus by the saline agglutination, antihuman globulin, or complement fixation test may be shown in subjects from the first two groups and generally there are no symptoms or signs of brucella infection whatever. Veterinary surgeons are prone to subclinical infection and young practitioners frequently display high titres against the usual tests at the same time apparently enjoying good health. Repeated reinfection of this group, however, may result in hypersensitivity to B. abortus and bouts of ill health at intervals may be due to this. Infection seems to come more often from contact with infected material than by drinking untreated milk, particularly in the herdsman, slaughterhouse worker, and veterinary surgeon. A doctor investigating illness of a patient from the dairy farming community and allied occupations should not accept a high titre against B. abortus as the only clue to diagnosis of the patient's condition.


Of toads and flowers.

Of toads and flowers.


Analysis of a protein fraction in the spore coats of Bacillus thuringiensis. Comparison with crystal protein.


Studies on the mode of action of nalidixic acid.


Survival, mating and rearing strategies in the evolution of primate social structure.


Quantitative and qualitative analysis of polychlorinated biphenyls by gas-liquid chromatography and flame ionization detection. I. One to three chlorine atoms.


Sai-1 mutation: saccharomyces cerevisiae: characteristics of inhibition by S-adenosylmethonine and S-adenosylhomocysteine and protection by methionine. The relationship of methionine to the inhibition caused by S-adenosylmethionine and S-adenosylhomocysteine in strains containing the sai-1 mutation has been investigated and shown to affect indirectly the survival of the mutants. The ability of the mutants to take up both inhibitors is similar to that of the wild-type cells. The mutant also retains the ability to hydrolyze S-adenosylhomocysteine and incorporate the hydrolytic products into the various cellular fractions. Maximal inhibition of the sai-1 mutants occurs at an extracellular concentration of 0.005 mmS-adenosylmethionine and 0.025 to 0.05 mmS-adenosylhomocysteine when the cellular concentration is 0.05 mg (dry weight) per ml. The results suggest that the sai-1 mutation affects reaction(s) either not associated with methionine biosynthesis, or methionine synthesis and at least one other critical cellular function.


Cloning of mouse myeloma cells and detection of rare variants.


Mechanical response with reversed electrical response to noradrenaline by Ca-deprived arterial smooth muscle. 1. When sheep carotid arteries had become electrically active after 30 min in Ca-free saline, noradrenaline 0.1 mM caused almost as much contraction as it did with Ca 1.25 mM present; it also caused slight electrical depolarization, usually with increased spike frequency, followed by electrical quiescence.2. In Ca-free saline with EDTA the arteries became profoundly depolarized, and their mechanical responses greatly reduced, within a few minutes. The mechanical responses to noradrenaline that remained were accompanied by electrical repolarization or, after longer periods in EDTA, by no electrical change.3. This residual mechanical responsiveness to noradrenaline, always tested at 36 degrees C, declined on average 26 times as rapidly during exposure to EDTA at 36 degrees C as at 5 degrees C and was not reduced by increasing the concentration of EDTA from 1.25 to 12.5 mM. This temperature-sensitivity was significantly too high to be explained by a diffusion-limited process.4. The results suggest that most of the tissue Ca responsible for contractility in simple Ca-free saline was either free in the extracellular space or in cellular stores that were discharged within a few minutes when free extracellular Ca was removed. They also indicate a small resistant Ca store which did not communicate with the exterior by diffusion, and part of which noradrenaline could utilize for contraction by means not dependent on depolarization conducted from the cell membrane.


The management of difficult cases of epilepsy with mandrax.


Ca concentration and flux in Ca-deprived arteries. 1. Extracellular Ca and EDTA concentrations in arterial smooth muscle in Ca-free solutions with and without EDTA were estimated from (45)Ca and [(14)C]EDTA washout studies. Ca concentrations were calculated from the measured rate of (45)Ca washout at a given moment, combined with separate determinations of the coefficient of diffusion of Ca in the tissue, while EDTA concentrations were calculated by analysing an exponential phase of [(14)C]EDTA washout and checked by separate determinations of coefficient of diffusion of EDTA in the tissue.2. Extracellular Ca concentrations after 30 min in simple Ca-free saline at 36 degrees C was 0.049 mM at the centre of the tissue and averaged 0.033 mM throughout the tissue; these were well above the value of 0.005 mM-Ca that was found to be the threshold external level for contraction after tissue stores of Ca were removed.3. Extracellular EDTA concentration at the centre of the tissue reached 6.25 mM after 1.9 min in EDTA 12.5 mM at 36 degrees C or after 4.9 min at 5 degrees C. It reached 12.45 mM after 30 min at 5 degrees C, enough to keep free extracellular Ca at this point well below threshold even if all of the Ca in the tissue, determined as 0.42 mumole/g, were suddenly released on warming.4. Ca efflux increased greatly on warming after long periods in cold EDTA, and all measurable Ca left the tissue within 30 min in EDTA 12.5 mM at 36 degrees C although tissue Mg did not fall significantly during this time.5. Contractions elicited by noradrenaline in Ca-free saline, with or without EDTA present, were not associated with any increases in the rate of external loss of Ca greater than 0.001 mumole.g(-1).min(-1).6. Electronmicrographs showed numerous microvesicles that communicated with the extracellular space; also smooth endoplasmic reticulum among other structures that might have contained non-communicating Ca stores.7. The results provide evidence that large responses given by the arteries to noradrenaline in simple Ca-free saline were due to persisting extracellular Ca or to labile Ca stores dependent on this, while small responses obtained after long periods in cold EDTA depended on non-communicating Ca stores whose loss was too temperature-dependent to be limited by diffusion.


Imaginal defects after UV-microbeam irradiation of early cleavage stages of Drosophila melanogaster.


Cardiovascular effects of the neuroleptics octoclothepin and oxyprothepin in animals.


Oxyphenon in the treatment of child enuresis.


Growth hormone. Control of release and characteristics in plasma.


Placental iron transfer: relationship to placental anatomy and phylogeny of the mammals.


Factor-dependent binding of Rauscher leukemia virus RNA to ribosomes.


Persisting circadian oscillations in enzyme activity in non-dividing cultures of Euglena.


Permeability properties of phospholipid membranes: effect of cholesterol and temperature.


Ratio circuit for plotting dose distribution in the radiation field of the 8 MV M.E.L. linear accelerator.


Is fast neutron radiotherapy simply a test of the oxygen effect?


Vasoactive actions of prostaglandins and serotonin on isolated human umbilical arteries and veins.


Humoral responses in human organ transplantation.


Studies on the first component of complement (C1) and the inhibitor of C1 esterase in rheumatoid synovial fluids.


The influence of different techniques in characterizing human antibodies to cow's milk proteins.


Induction of amyloidosis in germ-free NMRI-mice after prolonged stimulation with sterilized casein.


Murine thyroiditis. 3. Influence of syngeneic and allogeneic thyroid antigen on induction of the disease.


Characterization of the female-sterile mutant almondex of Drosophila melanogaster.


Actinomycin D-induced phenocopies in Drosophila melanogaster and their relevance to the time of gene action.


Family studies in systemic lupus erythematosus (SLE). 8. Serum immunoglobulins: IgG concentrations in relatives of selected SLE probands.


Determination of human serum immunoglobulins in healthy Thai subjects.


Expression of the mitochondrial genome in HeLa cells. X. Properties of mitochondrial polysomes.


Bacteriophage SP82G inhibition of an intracellular deoxyribonucleic acid inactivation process in Bacillus subtilis. The stability of SP82G bacteriophage deoxyribonucleic acid (DNA) after its uptake by competent Bacillus subtilis was examined by determining the ability of superinfecting phage particles to rescue genetic markers carried by the infective DNA. These experiments show that a DNA inactivation process within the cell is inhibited after infection of the cell by intact phage particles. The inhibition is maximally expressed 6 min after phage infection and is completely prevented by the addition of chloramphenicol at the time of infection. The protective effect of this function extends even to infective DNA which was present in the cell before the addition of intact phage. Continued protein synthesis does not appear to be a requirement for the maintenance of the inhibition. In an analogous situation, if infectious centers resulting from singly infecting phage particles are exposed to chloramphenicol shortly after the time of infection, an exponential decrease in the survival of infectious centers with time held in chloramphenicol is observed. If the addition of chloramphenicol is delayed until 6 min after infection, the infectious centers are resistant to chloramphenicol. The sensitivity of infectious centers treated with chloramphenicol at early times after infection is strongly dependent upon the multiplicity of infection and is consistent with a model of multiplicity reactivation. These results indicate that injected DNA is also susceptible to the intracellular inactivation process and suggest that the inhibition of this system is necessary for the successful establishment of an infectious center.


Visual exploratory behavior in the squirrel monkey (Saimiri sciureus).


Apparatus for programmed oral administration of drugs to large primates in altered environments.


The pattern of resistance development to rifampicin in Mycobacterium tuberculosis.


Rifampicin in the treatment of acute gonorrhoea.


Cell-mediated hypersensitivity to neural antigens. Occurrence in human patients and nonhuman primates with neurological diseases.


An experimental investigation into the relapse problem in vestibuloplasties with secondary epithelialization.


Thermodynamic studies of transfer ribonucleic acids. I. Magnesium binding to yeast phenylalanine transfer ribonucleic acid.


Paratyphoid in man and cattle.

Paratyphoid in man and cattle. Salmonella paratyphi B, phage type Taunton, caused generalized subclinical infection of cows and cases of human enteric fever at a dairy farm. The cattle were probably infected from a stream receiving the sewage effluent of a village in which a chronic carrier lived. At the same time as the farm outbreak a water-borne outbreak of human paratyphoid fever occurred in villages several miles away. The water supply was chlorinated and no failure of its treatment had been detected. S. paratyphi B, type Taunton, was isolated from the septic tank of a cottage near the water source and from soil over a break in the effluent pipe. Several inhabitants of the cottage worked at the infected dairy farm and one showed serological evidence of infection.Attention is drawn to the lack of statutory powers which could be applied to prevent the spread of the disease from the dairy herd and to the hazards of simple chlorination in the absence of additional safeguards of a water supply liable to pollution by surface water.


Multiple myeloma associated with a 9S IgG paraprotein and carcinoma of the prostate.


Treatment of head and neck cancer with intra-arterial cytotoxic drugs and radiotherapy.


Device to facilitate accurate measurement of intravenous fluid administration.


A prospective study of anti-candida interfering factor in leukemic patients.


Combined fractionated isotopic and external irradiation of the liver in Hodgkin's disease. A study of 21 patients.


Serum fetoprotein and malignancy in children.


Rapid quantitative evaluation of chemotherapeutic agents in experimental cystitis of rats.


The effect of emetine on macromolecular synthesis in synchronized tetrahymena pyriformis.


Clinical biochemistry of the baboon.

Clinical biochemistry of the baboon.


The use of laboratory animals in the potency test of Brucella abortus S.19 vaccine. Response of guinea-pigs to graduated doses of vaccine and challenge.


Intraocular lacrimal gland tissue with other ocular abnormalities occurring in a white-tailed deer.


Inducible antibacterial defence system in Drosophila.


The clinical significance of the presence of precipitin against tubercle bacilli in the sera of tuberculous patients.


The value of modified Löwenstein-Jensen medium for isolation of tubercle bacilli and of atypical bacilli from diagnostic material obtained from tuberculous children.


Late results of lung tissue resection in pulmonary diseases.


Early results of lung tissue resection in tuberculosis.


Analysis of the immunochemical relationship between antigens by electrophoresis in agarose gels containing antibodies.


Glomerular filtration rate determined by  51 Cr-EDTA-complex. A practical method based upon the plasma disappearance curve determined from four plasma samples.


Nutrition: a review of the WHO programme. II. The FAO, UNICEF and the WHO joined forces to deal with the problem of children suffering from protein-calorie malnutrition in many developing countries.  It was agreed that new or unconventional protein resources and processes required investigation and development, and major attention was given to the possibility of developing and testing substances for use as human food.  A Protein Advisory Group (PAG) was established by WHO in 1955 in recognition of the fact that the development of satisfactory foods from unconventional sources of proteins would involve special problems in food safety, toxicology, processing, and compatibility with infant diets.  The function of PAG is to advise the Directors-General of FAO and WHO and the Executive Director of UNICEF on matters relating to protein nutrition.  For an effective nutrition program, intensive studies of the etiology and pathogenesis of nutritional diseases and trials of preventive or therapeutic measures are necessary, and these are features of the WHO program of research in nutrition.  Protein-calorie malnutrition, nutritional anemias, and hypovitaminosis A are conditions to which high priority has been accorded in the research program.  The research efforts in the following areas of concern are reviewed: protein-calorie malnutrition, nutrition and infection, anthropometry as related to nutritional status, anemias, hypovitaminosis A, tropical sprue, endemic goitre, and rickets.


Organization of local and intermediate health administrations. Report of a WHO expert committee.


Disinfection of mechanical ventilators. An investigation using formaldehyde in a Cape ventilator.


Nutrition is the key to development.

Nutrition is the key to development.


Tridione and paradione as suspected teratogens.


The role of hydrogen and potassium ions in the transport of acidic amino acids in Staphylococcus aureus.


R factors in hospital infection.

R factors in hospital infection. In order to assess the clinical importance of R factors 524 "coliform" infections were studied in a general hospital. Of these, 95 were caused by Pseudomonas aeruginosa. Among the remaining 429, 43% were caused by drug-resistant enterobacteria; resistance was frequently multiple and determined by transmissible R factors. Choice of therapy was thus limited but in every case at least one antibiotic, effective in vitro, was still available.


A virus from epidemic vomiting disease.

A virus from epidemic vomiting disease. An illness consisting of vomiting, fever, and mild diarrhoea after a short incubation period was observed in a boarding school and reproduced in volunteers who received filtered extracts of faeces from a typical case. The main discriminatory diagnostic feature of the illness in volunteers was vomiting. The faeces contained no pathogenic bacteria nor any virus that could be detected in tissue cultures and there was no evidence that an infective agent could be grown in organ cultures of human intestine. The agent was shown to be ether stable and passed a 50-nm filter. Laboratory studies on another agent of uncertain significance and obtained in other epidemics are briefly described.


Hepatitis in dialysis units in the United Kingdom. A prospective study of hepatitis in 20 dialysis units in the United Kingdom showed that the number of units with outbreaks increased from one in 1968 to three in 1969 and the overall attack rate rose from 1.7 to 5.3% in patients and from 0.5 to 1.3% in staff.In July 1969 a pilot study of patients' sera showed that all of the outbreaks were associated with Australia (Au) antigen. Au antigen was not detected in the unaffected units or in one unit in which there were several patients with abnormal liver function.In January 1970 systematic prospective testing for Au antigen was begun. Au antigen was detected in single patients in five more of the 20 units. The transfer of the affected patients to isolation for dialysis seemed to prevent the spread of infection in the units. The incidence of hepatitis in 1970 was 5.6% in patients and 0.4% in staff.


Aortic arch anomalies: simplified classification: surgical experience with ten cases.


Appearance of myosin in the chick limb bud.


Events surrounding the early development of Euglena chloroplasts. II. Normal development of fine structure and the consequences of preillumination.


Ectodermal-mesodermal interactions in the growth of limb buds in the chick embryo: constancy and temporal limits of the ectodermal induction.


Serum inactivation of the immunological and biological activity of thyrotropin releasing hormone (TRH).


The corpus luteum of the rhesus monkey during late pregnancy.


Plasma thyrotropin responses to thyrotropin-releasing hormone after injection into the third ventricle, systemic circulation, median eminence and anterior pituitary.


Differences in the rat uterine weight-stimulating capacity of rat luteinizing hormone and other LH's: the non-physiologic nature of bovine and ovine LH in the rat.


Immunochemical heterogeneity of commercial hog pepsinogen.


Interaction of insulin and amino acid metabolism in the regulation of gluconeogenesis.


Economic worth of preventing death at different ages in developing countries.


The occurrence of bactoprenol in the mesosome and plasma membranes of Lactobacillus casei and Lactobacillus plantarum.


Purification and properties of carotene 15,15'-dioxygenase of rabbit intestine. Carotene 15,15'-dioxygenase, which oxidizes carotenoids to retinal, has been purified up to 200-fold from rabbit intestine by ammonium sulfate fractionation, heat treatment, and acetone precipitation. With beta-apo-10'-carotenol as the substrate, the purified enzyme has a pH optimum of 7.8, a K(m) of 6.7 x 10(-5) m, and a V(max) at 37 degrees C of 9 nmoles of retinal/mg protein/hr. The purified enzyme is inhibited by ferrous ion-chelating agents such as alpha,alpha'-dipyridyl and o-phenanthroline, and by sulfhydryl-binding agents such as iodoacetamide, N-ethylmaleimide, and p-chloromercuribenzoate. The latter inhibitory effects are reversed by reduced glutathione. The cleavage of beta-apo-10'-carotenol is competitively inhibited by its acetylenic analog, 15,15'-dehydro-beta-apo-10'-carotenol. The enzyme is present in the intestinal mucosa of several mammals, the chicken, the tortoise, and a freshwater fish, but it is absent from cat intestinal tissue.


Base exchange reactions of the phospholipids in rat brain particles. A particulate fraction from rat brain catalyzes the incorporation of [(14)C]choline, [(14)C]ethanolamine, and l-[(14)C]serine into phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine, respectively. The reaction appears to be energy-independent since Mg(2+), CTP, ATP, and NaF have no stimulatory action. The incorporation is inhibited by EDTA and activated by Ca(2+). The pH optimum for the incorporation of choline is 9.5, for ethanolamine it is 9.0, and for l-serine it is 8.5. Tris, bicine, and imidazole buffers are inhibitory. The incorporations are inhibited by a variety of structurally related alcohols and are stimulated by isoserine (alpha-hydroxy,beta-aminopropionic acid).


Are the genes controlling cytokinesis late-replicating and located on the X-chromosome?


Dissociation and re-association of HeLa cell ribosomes.


Excretion of nicotine and its metabolites in dog and monkey saliva.


The density and ultrastructure of the Purkinje cells following diphenylhydantoin treatment in animals and man.


Unconjugated and conjugated bilirubin in plasma from patients with erythroblastosis and neonatal hyperbilirubinemia.


Preparation and use of RNA-cellulose columns.


The effect of local anesthetics on the hydrolysis of free and membrane-bound phospholipids catalyzed by various phospholipases.


Studies on human pancreatic lipase. I. Interconversion between low and high molecular forms of human pancreatic lipase.


Sphingolipid base phosphorylation by cell-free preparations from Tetrahymena pyriformis.


Immunoglobulins and antibody to Loa loa in Nigerians with endomyocardial fibrosis and other heart diseases.


Effect of tetracyclines and 4-epiderivatives on the ureter. 1. The effect of tetracyclines on the isolated dog ureter is dependent on: (a) the tetracyclines used-mepicycline and doxycycline antagonizing, and tetracycline and rolitetracycline increasing the contractor action of barium chloride; (b) the percentage of 4-epiderivatives in the tetracyclines used-the higher the epiderivative concentration, the smaller the effect of mepicycline or doxycycline, and the greater the action of tetracycline or rolitetracycline.2. In vivo the addition of the antibiotics into the renal pelvis shows no significant differences between the various tetracyclines or different 4-epiderivative concentrations on the intra-ureteral flow of the dog or guinea-pig.3. Intravenous injection of mepicycline or doxycycline does not induce a significant change in the intra-ureteral flow, while intravenous administration of tetracycline or rolitetracycline produces a triphasic response: (a) a marked decrease of the intra-ureteral flow for a few minutes; (b) a return to the control condition for 30-60 min; and (c) a lesser but persistent decrease in flow for 60-120 minutes. In the first phase the ureteral smooth muscle is directly affected by the antibiotics circulating in the blood, while in the third phase the tetracyclines act via the intra-ureteral mucosa.4. Neurogenic effects on the ureter-bladder junction in vivo are not affected by the tetracyclines tested.


AH 8165: a new short-acting, competitive neuromuscular blocking drug.


Origin of the after-hyperpolarization that follows removal of depolarizing agents from the isolated superior cervical ganglion of the rat. 1. Potential changes in isolated rat superior cervical ganglia following addition and removal of depolarizing agents were recorded using a moving-fluid extracellular electrode system.2. Ganglionic negativity produced by carbachol was followed by a pronounced ganglionic positivity on washing. This after-positivity was attributed to hyperpolarization of the ganglion cells since it was unaffected by crushing the postganglionic trunk.3. The after-hyperpolarization was selectively depressed by (a) cooling (Q(10) 2.3), (b) metabolic inhibitors (cyanide, azide, 2,4-dinitrophenol), (c) reducing [K(+)](o) or substituting Cs(+) for K(+), (d) ouabain, and (e) substituting Li(+) for Na(+). This suggested a close dependence on active Na(+) transport.4. When K(+) was restored to K(+)-free solution, or the preparation was warmed rapidly, or when metabolic inhibitors were washed away, the hyperpolarization was rapidly regenerated. The effect of restoring K(+) indicated that the hyperpolarization was generated directly by the Na(+) pump.5. The hyperpolarization was not altered by replacing Cl(-) with isethionate, indicating that the voltage change produced by the Na(+) current was not modified by passive Cl(-) movements.6. Hexamethonium added to the washout fluid augmented the after-hyperpolarization, suggesting that there was a high (cationic) leak current due to continued receptor-activation on washing with normal Krebs solution.7. The hyperpolarization was reduced by omission of Ca(2+) and restored by addition of Mg(2+). This was considered to result from changes in passive membrane permeability.8. The time-course of post-carbachol hyperpolarization accorded with a Na(+) extrusion process whose rate was directly proportional to [Na(+)](i) with a rate constant of 0.38+/-0.02 min(-1) at 23-27 degrees C.9. With increasing concentrations of carbachol, the amplitude of the hyperpolarization increased in proportion to the preceding depolarization, but the rate constant of the hyperpolarization was unchanged.


A method for providing intermittent intravenous injections in unrestrained animals.


Relative importance of the enzymic hydrolysis of sexamethonium in plasma and tissues: studies in Rhesus monkeys. 1. Suxamethonium was given by intravenous infusion to anaesthetized Rhesus monkeys and the infusion rate required to produce after 10 min a 50% reduction in twitch tension of the indirectly stimulated gastrocnemius or tibialis anterior muscle (IR50) was calculated from results obtained with paired infusions of two concentrations, repeated at intervals. The IR50 values in three monkeys were (388, 958 and 1,010 nmol/kg)/minute.2. In one experiment the cholinesterase activity in plasma was increased to 125 and then to 182% of the initial value by infusions of purified human cholinesterase (usual enzyme). This increased the IR50 by 34 and then by 60%, respectively.3. Inhibition by iso-OMPA of cholinesterase activity in plasma and tissues (four monkeys) by more than 90% lowered the mean IR50 value to (69 +/- 11 (S.E.M.) nmol/kg)/minute. Restoration of cholinesterase activity in the plasma of these animals by infusion of purified human cholinesterase raised the IR50's to values within the normal range.4. It is concluded that in the Rhesus monkey most of the infused suxamethonium is hydrolyzed by the cholinesterase in plasma before reaching the motor endplates. This agrees with observations in man with usual cholinesterase and contrasts with experiments in the cat where the hydrolysis of suxamethonium by cholinesterase in tissues was found to be more important than the hydrolysis by cholinesterase in plasma.


The feudal caste structure of health care.


Characterization and purification of a bovine Bence-Jones protein.


Purification and properties of an antigen cross-reacting with Escherichia coli  -galactosidase.


Species specificity in response to tryptophan modified encephalitogen.


Mutagenic properties of N-acetyl-2-aminofluorene and its metabolites in relation to the molecular mechanisms of carcinogenesis.


Electroosmophoretic radioimmunoassay: application to hepatitis-associated antigen.


Radioactive urokinase for blood clot scanning.


The pharmacology of the antimalarial drug 4,4'-diformamidodiphenylsulfone (DFD) in dog and monkey.


Clearance and metabolism of organic nitrates.


Diagnosis of Pneumocystis carinii pneumonitis by means of endobronchial brush biopsy.


Hypothalamic hyperthyroidism (?).

Hypothalamic hyperthyroidism (?).


Present status of contact lenses.

Present status of contact lenses.


Rabies immunization.

Rabies immunization.


Access to medical care for the poor: the Federal perspective.


Gypsy moth control with the sex attractant pheromone.


Retina: ultrastructural alterations produced by extremely low levels of coherent radiation. The effect of low levels of coherent radiation on the eye is not fully established, but is generally presumed to be noninjurious. Irradiation of the retina with a Q-switched ruby laser emitting low amounts of energy (0.1 percent probability of creating damage) consistently produces ultrastructural alterations in rods and cones. Outer segments of these cells are broken and disorganized and their lamellae are in disarray 1 day after such irradiation.


Membrane abnormalities in polyagglutinable erythrocytes.


Iron metabolism and red cell survival in uremic patients.


Haemoglobin E-hereditary elliptocytosis in Malayan aborigines.


Anaemia and erythrocyte transketolase activity.


Congenital methemoglobinemia due to hemoglobin M.


Localization of erythropoietin in the glomerulus of the hypoxic dog kidney using a fluorescent antibody technique.


Antimetabolite therapy in the post-myocardial-infarction syndrome.


Fractionation of DNA from Bacillus subtilis and its transforming activity for various markers.


The calcium fractions in the plasma of patients on dialysis.


Nuclear magnetic resonance studies of selectively hindered internal motion of substrate analogs at the active site of pyruvate kinase.


Formation of the  -( -glutamyl) lysine cross-link in hair proteins. Investigation of transamidases in hair follicles.


Amino acid sequence of monkey amyloid protein A.


Purification and characterization of two classes of immunoglobulins from the marine toad, Bufo marinus.


Orientation restrictions of the peptide hormone, thyrotropin-releasing factor, due to intramolecular hydrogen bonding.


Bacterial endocarditis due to Listeria monocytogenes.


Klossiella equi Baumann, 1946 (Sporozoa: Eucoccidia: Adeleina) from equids.


Quinine inhibition of host cell penetration by Toxoplasma gondii, Besnoitia jellisoni, and Sarcocystis sp. in vitro.


Fine structure of Haemobartonella sp. in the squirrel monkey.


Morphometric estimation of pulmonary diffusion capacity. V. Comparative morphometry of alveolar lungs.


A radioimmunoassay for progesterone.

A radioimmunoassay for progesterone.


Polarographic reduction of oxygen in the presence of phthalocyanine complexes.


Dehydro N-mannich bases.

Dehydro N-mannich bases.


Tetraorganofluorophosphoranes.

Tetraorganofluorophosphoranes.


Tetramethyl(methoxy) phosphoranes and related compounds.


Synthesis and pulse Fourier transform  13 C-NMR spectra of branched-chain carbohydrates.


Synthesis of branched carbohydrates with aldehyde side-chains. Simple synthesis of L-streptose and D-hamamelose.


Evaluation of assay methods for hepatitis-associated antigen. Assay methods for hepatitis-associated antigen (HAA) were evaluated for sensitivity, or reproducibility, or both in a series of three trials in which both research and service-oriented laboratories participated. Agar-gel diffusion (AGD) methods were found to be the least sensitive and reproducible of the commonly employed assay methods. Complement fixation (CF) tests were consistently more sensitive than either AGD or counterelectrophoresis (CEP) methods for detection of HAA. With judicious choice of the antibody reagent, sensitivity of CEP techniques was equivalent to CF methods of HAA detection. None of the three major assay methods (AGD, CEP, or CF) compared in this study were capable of consistently detecting HAA when it was present in relatively low concentrations in human serum.


Hermann J. Muller, 1890-1967. An appreciation by a friend.


Quantitative measurement of precipitating antibodies in streptococcal grouping antisera by the single radial immunodiffusion technique. A comparative study was made of the single radial immunodiffusion test and the classical quantitative precipitin test for determining the amount of precipitable antibodies present in streptococcal groups A and C antisera. The potency of 21 group A and 54 group C antisera was determined by both methods; purified group-specific carbohydrates were used as antigens. The coefficient of correlation between the results from the two methods was 0.976 for group A antisera and 0.946 for group C antisera. When the concentration of antigen, the volume of antiserum used, and the depth of the antigen-agar mixture are kept constant, the diameter of the precipitin disc is directly related to the concentration of precipitable antibodies present in the antiserum. The use of the radial immunodiffusion test for evaluating and standardizing streptococcal grouping antisera is discussed as well as the advantages and disadvantages of using a concentrated vaccine for producing these antisera.


Loss of lactose metabolism in lactic streptococci. Lactose-negative mutants occurred spontaneously in broth cultures of Streptococcus lactis C(2)F. Instability of lactose metabolism was noted in other strains of S. lactis, in strains of S. cremoris, and in S. diacetilactis. Colonies of S. lactis C(2)F grown with lactose as the carbohydrate source also possessed lac(-) cells. Treatment of lactic streptococci with the mutagen acriflavine (AF) increased the number of non-lactose-fermenting variants. The effect of AF on growth and on loss of lactose-fermenting ability in S. lactis C(2)F was consequently further examined. The presence of AF appears to favor competitively the growth of spontaneously occurring lactose-negative cells and appears to act in the conversion of lactose-positive to non-lactose-fermenting cells. The lactose-negative mutants partially revert to lactose-positive variants which remain defective in lactose metabolism and remain unable to coagulate milk. The lactose-negative cells become dominant in continuous culture growth and provide evidence that alterations in the characteristics of starter strains can be produced by continuous culture, in this case, the complete loss in ability to ferment lactose.


Dry heat inactivation of Bacillus subtilis var. niger spores as a function of relative humidity. Dry heat sterilization of Bacillus subtilis var. niger spores at 105 C is enhanced in the relative humidity range 0.03 to 0.2%. D-values of 115 and 125 C are predicted by a kinetic model with parameters set from 105 C data. These predictions are compared to observations.


Simple liquid scrubber for large-volume air sampling. A new large-volume air sampler called the "simple liquid scrubber" is described. It can recover a high percentage of microorganisms from large volumes of air, up to 950 liters/min, and concentrate them into a small volume of liquid at a ratio of about 400,000 to 1. The principle of operation of the scrubber is based on the production of a fine mist in a rapidly moving airstream with ultimate collection of the airborne particles by impingement into the film of liquid formed upon impaction of the mist droplets on the scrubber walls. The scrubber compared favorably with the all-glass impinger (AGI-30) and the slit sampler in tests with the normal flora and with experimental aerosols of Bacillus subtilis var. niger spores.


Scanning electron microscopy of microbial cells on membrane filters. Scanning electron micrographs of a Pseudomonas species, Staphylococcus aureus, and Bacillus subtilis on two membrane filtration systems are compared.


Physiologic basis of emotional expression: evoked potential and mirror focus studies in rhesus monkeys.


A new antibiotic, ikarugamycin.

A new antibiotic, ikarugamycin.


Jolipeptin, a new peptide antibiotic. II. The mode of action of jolipeptin.


Regulation of hexokinase isozymes: apparent interconversion between Type II and Type 3 or inactive enzyme complex formation in rat tissues.


Mechanisms for the efflux of [14C]DOPA and[14C]dopamine from the CSF of rhesus monkeys.


Gonorrhea as a cause of asymptomatic pyuria in adolescent boys.


Hypercalcemia as a complication of parenteral alimentation.


Changes in serotype of Pseudomonas aeruginosa.


Reactants to human serum albumin-coated red cells found in Au(1)-positive sera.


Unusual complications following cervical esophagostomy.


Gastric carcinoid with ossification.

Gastric carcinoid with ossification.


Surgical treatment of chronic pancreatitis.


Superior mesenteric artery syndrome in burned children.


Repetitive peer separations of juvenile-age rhesus monkeys.


Euglena gracilis cytochrome 558.

Euglena gracilis cytochrome 558.


Intraveneous hyperalimentation: indications, technique, and complications.


The carboxyl-terminal amino acid sequence of Streptomyces griseus protease A.


The separation of two deoxyribonucleases from extracts of intestinal mucosa of the rat.


Complement studies in membrano-proliferative glomerulonephritis.


Serum immunoglobulin levels in Australia antigen positive and Australia antigen negative hepatitis.


Purification and immunochemical characterization of group II pepsinogens in human seminal fluid.


Lymphocyte-monocyte defect associated with anergy and recurrent infections.


Specificity and properties of anti-adenohypophysis serum. I. Mode of preparation, titration and effects of neonatal injection into rats.


Immunoglobulin-containing cells in the intestinal mucosa and immunoglobulins in the intestinal juice in children.


Effect of calcitonin on experimental osteolathyrism. (3). Selective inhibition of collagen resorption by calcitonin.


Ophthalmia neonatorum is still with us.

Ophthalmia neonatorum is still with us.


Pollution disasters in Japan.

Pollution disasters in Japan.


Law and medicine: reflections on a metaphysical misalliance.


The measurement of the transfer of calcium and phosphorus from foetus to dam in the sheep using a whole body counter.


Single cell activity in the auditory cortex of Rhesus monkeys: behavioral dependency. The response to repetitive stimulation of single cells in the auditory cortex of the monkey is dependent upon behavioral performance and training of the subject in a simple auditory discrimination task. In the trained, performing animal, single cells are more responsive than in the animal that is trained but not performing in the task. In the naive monkey, evoked responses are labile and are maintained only with nonrepetitive auditory stimuli.


A new biomicrogonioscopy. A clinical approach to the functioning fine structure of the chamber angle.


Fluctuations in the polysome content of gamma-irradiated Tetrahymena pyriformis.


Effects of aldosterone on RNA and protein synthesis in the toad bladder.


Kinetic study of the activation process of  -galactosidase from Escherichia coli by Mg 2+ .


Nuclease degradation and the structure of ribosomes.


Development, distribution, and lipid content of diet-induced atherosclerotic lesions of rhesus monkeys.


The occurrence of folic acid (pteroyl-L-monoglutamic acid) in human blood serum after small oral doses. Human serum folates analysed by bioautography after oral doses of 0.5 mg folic acid (pteroyl-L-monoglutamic acid) show folic acid to be present.


The relation of total body potassium to height, weight, and age in normal adults. Total body potassium was measured in 103 healthy adults using a shadow-shield whole-body monitor of high sensitivity. The range of height was 147 to 192 cm, of weight 43 to 92 kg, and of age 18 to 77 years. The values obtained for total body potassium were correlated with height, with weight, and with height and weight. Age was then included as an additional variable. The standard deviation from regression was smaller when total body potassium was correlated with height than with weight and was further reduced, to about 9%, in a multiple regression using height and age. The advantages of this relationship over indices involving weight are discussed. The smallest standard deviation from regression, 7.5%, was obtained when total body potassium was correlated with height, weight, and age. The usefulness of this relationship is discussed with comment on its limitations.A regression equation was derived between lean body mass (derived from height and weight) and total body potassium with a standard deviation from regression of 5.5% in males and 7.3% in females.


Serotype and sulphonamide sensitivity of meningococci isolated from 1966 to 1971. From 1966 to 1971, 298 cultures of meningococci from clinical material (cerebrospinal fluid or blood) were examined. Eighty-nine cultures were from the Manchester area and the remainder from other parts of England or Northern Ireland. Five per cent of strains were group A, 57% group B, and 31% group C; 6% were untypable. Eighteen strains (6%) had an MIC of 6.4 mug/ml or more of sodium sulphadiazine and 10 of these (3.5%) an MIC of 50 to 100 mug/ml. The incidence of sulphonamide resistance was higher in group A strains than in group B or group C strains.


The isolation of influenza viruses.

The isolation of influenza viruses. Of 129 strains of the Hong Kong variant of influenza A2 virus isolated from 176 respiratory infections, 105 (81.4%) were isolated in tissue culture, the remainder being detected only by the inoculation of fertile hen's eggs. Organ cultures of chick embryo trachea were at least as sensitive as monkey kidney tissue culture for the isolation of this virus but organ cultures of human embryonic ciliated epithelium were much less efficient. The reverse was true for strains of influenza B virus studied in 1970 and 1971 when eggs and chick embryo tracheal organ culture were of little value and organ cultures of human embryonic ciliated epithelium and monkey kidney tissue cultures were the systems of choice.