Variations in the output impedance of the human motor system.


Variations of arterial oxygen and carbon dioxide tensions during 24 hours in chronic respiratory insufficiency.


Varicella-like rash associated with Mycoplasma infection.


Various vagaries of vasoconstriction.

Various vagaries of vasoconstriction.


Vascularization of skeletal muscles in some Caudata.


Venezuelan Macronyssidae. 3. Chiroecetes lonchophylla, n. g., n. sp., from a long-tongued bat.


Vasopressors for treating shock.

Vasopressors for treating shock. Shock must be treated by correcting the cause, for any treatment of hypotension or shock, as such, is only an adjunctive measure; but the hemodynamic manifestations also need treatment.  Vasopressors are helpful and effective under the right circumstances.  Unless the blood volume is normal, the use of drugs that block the sympathetic nervous system (e.g., phenoxybenxamine) can be extremely hazardous and hasten death.  However, the effect of adrenergic blocking drugs in endotoxic shock and other types of toxic shock is still to be determined; use of such drugs should be considered experimental until the results have been studied more extensively.  Clinically, the most common forms of vascular shock are associated with blood loss, myocardial infarction, and endotoxemia.  Characteristic hemodynamics of each situation are presented tabularly, and the physicians need to understand the differences is emphasized.  The pharmacology of vasopressors, relating primarily to hemodynamic considerations and the response to vasopressors when severe reduction in blood pressure is associated with the shock syndrome is discussed.  Drugs that stimulate the adrenergic receptors in the heart and blood vessels, with the exception of isoproteronol, are commonly referred to as vasopressors.  The adrenergic stimulators may be classified into 3 groups: alpha (phenylephrine hydrochloride), beta (epinephrine), and alpha-beta (l-norepinephrine).  Because alpha stimulators do not usually increase cardiac output, alpha-beta and beta-adrenergic stimulators are generally the most useful for treating shock.  Routine use of adrenergic stimulators with the exclusion of other therapies, however, is generally unwarranted.


Vectorial electron flow across the thylakoid membrane. Further evidence by kinetic measurements with an electrochromic and electrical method.


Ventilation distribution in anatomical lung units.


Venoconstrictor effect of dihydroergotamine in superficial hand veins.


Ventilation rate and end--tidal steady state pulmonary diffusing capacity (DLCOSS2) at rest.


Venting overflow gases from the Air Shields (Ventimeter) ventilator.


Ventilatory mechanics in patients with bronchiectasis starting in childhood.


Ventricular fluid pressure recording after severe brain injuries.


Verrucous carcinoma of the buccal mucosa.


Vertebral plasma cell myeloma causing posterior paralysis in a horse.


Vesicocolic fistula.

Vesicocolic fistula.


Visceral viewpoints. Moynihan's disease? The diagnosis of duodenal ulcer.


Viral proteins formed in a cell-free rabbit reticulocyte system programmed with RNA from a temperature-sensitive mutant of Sindbis virus. Viral messenger RNA was isolated from BHK cells infected with a temperature-sensitive mutant of Sindbis virus and was further purified using an oligo(dT) column. Addition of this mRNA cell-free extracts from rabbit reticulocytes led to formation of discrete authentic viral capsid protein when the reaction was performed at 29 C. However, this same protein-synthesizing system failed to make discrete viral capsid when incubated with the viral RNA at 39 C. Instead, larger-molecular-weight polypeptides that contained the viral capsid peptide sequences were produced. The inability to make a separate viral capside protein in vitro at elevated temperatures by the mRNA from this mutant exactly mimics the phenotype of this ts mutant in viral-infected cells. Three mechanisms are discussed that might account for a temperature-sensitive release of capsid. One of these is based on a model in which there are multiple sites for initiation of translation of polypeptides on a polycistronic viral mRNA.


Visceromegaly in acromegaly. Evidence that clinical hepatomegaly or splenomegaly (but not sialomegaly) are manifestations of a second disease.


Visual evoked responses (VERs) in normal and disabled readers.


Visual fields of butterfly interneurons.


Visual neglect in a chess player.

Visual neglect in a chess player.


Vitamin A metabolism under nutritional stress.


Vitamin B12 and the macromolecular composition of Euglena. 3. Effect of cycloheximide on the recovery from B12-induced unbalanced growth. When cycloheximide is added to (B12)-deficient cultures before or after replenishment of the cells with B(12), reversion of these cells is inhibited. This inhibition is not caused by interference of the inhibitor in the uptake of B(12) as measured by division kinetics. Cycloheximide does not inhibit the initial increase in the rate of DNA synthesis caused by B(12) replenishment, but within 30-45 min the rate decreases and DNA synthesis ceases. Cycloheximide added to replenished deficient cells after completion of DNA duplication inhibits cell division. The total cellular protein and RNA in replenished cells treated with cycloheximide does not change. B(12) added to deficient cells does not stimulate the incorporation of [(14)C]leucine into protein during resumption and completion of DNA duplication. However, there is a large increase in [(14)C]leucine incorporation into the protein of these cells soon after completion of DNA duplication and before resumption of cell division. The addition of cycloheximide to B(12)-replenished or to nonreplenished deficient cells rapidly inhibits the incorporation. We suggest that the addition of B(12) accelerates the rate of DNA synthesis in the deficient cells and that possibly no new protein synthesis is required except for mitosis. However, protein synthesis is needed for continuous DNA synthesis.


Volume control and compartment analysis in multiple-breath nitrogen washout.


Visible laryngeal changes during voice therapy: fiber-optic study.


Voluntary hospitals covered by Taft-Hartley Act: a new era in hospital labor relations.


Wallenberg's syndrome. I. General symptomatology, with special reference to visual disturbances and imbalance.


Wallenberg's syndrome. II. Oculomotor and oculostatic disturbances.


Vestibular and cochlear neurectomy.

Vestibular and cochlear neurectomy.


Warfarin and ascorbic acid: lack of evidence for a drug interaction.


Water quantity and quality management in the Ruhr valley.


Warfarin-chloral hydrate interaction. Pharmacological activity and clinical significance.


Vocal rehabilitation in the laryngectomized patient with a tracheoesophageal shunt.


Water transport across isolated term human amnion.


Wegener's granulomatosis occurring in a patient with pre-existing rheumatoid arthritis.


Weight of the femur--a useful measurement for identification of sex.


Weight-height indices. Choice of the most suitable index and its association with selected variables among 10,000 adult males of heterogeneous origin.


What doctors across the country think of New York's tough new narcotics law.


What has been the impact so far?

What has been the impact so far?


Voice prosthesis for speech restoration in laryngectomees.


What is diagnostic radiology's place in medicine? The question that the title of this lecture poses must depend considerably on the attitude of physicians and surgeons. I have indicated the very low position diagnostic radiology holds in this country relative to our peers in medicine elsewhere.If its improvement is considered to be warranted, we must:(1) Interest medical students at the beginning of their career.(2) Bear in mind that radiologists are likely to be able to teach some anatomy but the reciprocal seldom applies.(3) Obtain chairs in radiology, which are desperately needed.(4) Obtain the acceptance by the medical establishment of the proper place of radiology in clinical medicine.(5) See to the reduction in numbers of unnecessary x-ray examinations.(6) Press for the improvement and enlargement of radiological departments with proper provision for expansion.


What is the question?

What is the question?


What the law says about prescribing narcotics, methadone, and other controlled substances.


What's happening to biomedical engineering in Canada?


When handwriting is a handicap.

When handwriting is a handicap.


Where we stand on drug abuse.

Where we stand on drug abuse.


Who will teach the basic medical sciences? In the United States, circumstances still permit each university to set its own course under relatively broad and generous guidelines. The options for our faculties are relatively unrestricted and they can play important roles in determining university policy. We need never reach a stage which nmany can label "crisis,"if events are predetermined by appropriate planning. The survey described herein points to the need for specific long-range planning of the future of preclinical departments in each university. The overall health care system is clearly involved in the problems described in Britain. The coming of a new order of health care in the United States should cause planners to accelerate their work.


Wide spectrum of motor conduction velocity in Charcot-Marie-Tooth disease. An anatomico-physiological interpretation.


Wives' mental health and children's behaviour problems in contrasting residential areas.


Wka (Weeks), a new antigen in the Kell blood group system.


Work behavior, time urgency and life dissatisfactions in subjects with myocardial infarction: a cross-cultural study.


Wound dressing or window dressing?

Wound dressing or window dressing?


Work-induced hypertrophy in exercised normal muscles of different ages and the reversibility of hypertrophy after cessation of exercise. 1. Groups of male hamsters of different ages were subjected to a weight-lifting exercise regimen, and the biceps brachii, soleus and extensor digitorum longus (EDL) muscles examined for structural changes occurring in response to the increased workload. In addition, two groups of adult hamsters were left to recover from the exercise stimulus for 5 and 15 weeks respectively.2. All the exercised muscles exhibited muscle fibre hypertrophy, and the extent of the hypertrophy was greater in the younger animals. In all age groups, the biceps brachii and EDL showed more hypertrophy than did the soleus. There was no significant increase in fibre number after exercise.3. In both groups allowed to recover from the exercise stimulus, the fibre dimensions reverted back to those of the control muscles; this appeared to be complete after 15 weeks recovery.4. Electron microscopical studies of fibres from exercised and control biceps brachii revealed no significant changes with exercise in the proportions present of myofibrillar, mitochondrial and tubular components within muscle fibres of the same size.


Writing for the journal.

Writing for the journal.


Whither American science?

Whither American science?


X-ray beam uniformity in 250 kV deep therapy units.


X-ray conference: Spine changes in a 20-year-old black male.


X-ray diffraction study of membrane fragments rich in acetylcholine receptor protein prepared from the electric organ of Torpedo marmorata.


Xanthoma disseminatum.

Xanthoma disseminatum.


X-ray evidence for metal-N-7 bonding in a hydrated manganese derivative of guanosine 5'-monophosphate. Single-crystal X-ray studies of a manganese(II) derivative of guanosine 5'-monophosphate, [Mn(5'-GMP)(H(2)O)(5)],3H(2)O, have shown that it is isostructural with its nickel analogue. The manganese atom therefore is bonded to five water molecules with the remaining octahedral co-ordination site being occupied by N-7 of the nucleotide base. No direct metal-phosphate bonding is involved, but there are structure-stabilizing intramolecular hydrogen bonds between two phosphate oxygen atoms and co-ordinated water molecules.


Xanthomatous ureteral polyp.

Xanthomatous ureteral polyp.


Xeroderma pigmentosum with liver involvement.


Your business. Is leasing a gimmick or a good idea?


Zervamicins I and II, polypeptide antibiotics produced by emericellopsis salmosynnemata.


1H nmr study on the binding of CMP inhibitors to RNase A. 3. Chemical exchange and relaxation effects.


Zinc and copper in serum and urine of children with burns.


13 C-nuclear magnetic resonance and x-ray photoelectron spectroscopy of Cu-AMP.


13C nuclear magnetic resonance study of the spatial relation of the metal- and anion-binding sites of human transferrin.


13C-nuclear magnetic resonance relaxation measurements of alpha-lecithin--peptide interaction in model membranes.


13C-13C coupling constants in a series of 13C-enriched amino acids.


14-3-2 Protein in rat brain synaptosomes: an immunochemical study.


19-Hydroxylated E prostaglandins as the major prostaglandins of human semen.


20-year mortality of tetraethyl lead workers.


3H-thymidine incorporation into circulating white cells during allergic and irritant dermatitis in guinea pigs.


5-FU in chick embryos as a source of label for DNA and a depressant of protein synthesis.


3Alpha-androstanediol kinetics in man.

3Alpha-androstanediol kinetics in man. Kinetics of 5alpha-androstane-3alpha, 17beta-diol (3alpha-diol) were studied in man. Clearance rates were determined by both the constant infusion and single injection techniques. Production rates were calculated as the product of clearance rate data and plasma values in the a.m. obtained by a radioimmunoassay specific for 3alpha-diol. Mean metabolic clearance rates were 1,776+/-492 (SD) liters/day in males and 1,297+/-219 (SD) liters/day in females. Metabolic clearance rates by single injection were similar. Calculated production rates are 208+/-26 (SD) mug/day in males and 35+/-11 mug/day in females, which are significantly different. Hepatic extraction of 3alpha-diol determined by hepatic vein catheterization during constant infusion was 76% which was greater than expected from information on in vitro binding in plasma. The kinetic data is of interest since 3alpha-diol has a calculated inner pool (V(1)) volume of 12-14 liters, similar to 17beta-hydroxyandrost-4-en-3-one (testosterone) and 5alpha-androstan-17beta-ol-3-one (dihydrotestosterone), but the calculated outer pool (V(2)) of 33.5 liters is very large as are the metabolic rate and transfer constant. In contrast to testosterone and dihydrotestosterone, 3alpha-diol, although bound to sex hormone binding globulin, has a high metabolic clearance of which a large fraction represents extrahepatic (splanchnic) metabolism. A production rate of 3alpha-diol similar to dihydrotestosterone together with rather unique kinetic characteristics encourages further investigation of the biological role of this potent androgen.


5-Hydroxytryptamine metabolism in joint diseases.


67Ga-scintigraphy in malignant lesions of the lung.


67Ga-uptake in the regenerating rat liver.


7-Oxo-delta1-tetrahydrocannabinol: a novel metabolite of delta1-tetrahydrocannabinol.


8-Azaadenosine. Crystallographic evidence for a "high-anti" conformation around a shortened glycosidic linkage.


89-Sr for the treatment of incurable pain in patient with neoplastic osseous infiltrations.


3. Drug regulations in Switzerland and their application.


Pressure gun injuries to the hand.

Pressure gun injuries to the hand.


Drug evaluation--the every-day-work of the clinical pharmacologist.


A comparative gaschromatographic study on absorption of diazepam tablets in man. A gaschromatographic study with Ni63-detector by determining diazepam concentrations in plasma.


Bioavailability of the acetylsalicylic acid tablets registered in Finland.


Biopharmaceutical assessment of phenylbutazone and indomethacin preparations.


Distribution of impaired cutaneous vasomotor and sudomotor function in paraplegic man.


Recording of normal and impaired cutaneous thermoregulatory vasomotor responses by infra-red thermography: a methodological study.


Choice of subjects for in vivo studies in generic equivalency.


(1-beta-mercapto-beta,beta-diethylpropionic acid,4-leucine)-8-lysine-vasopressin and (1-beta-mercapto-beta,beta-diethylpropionic acid,4-leucine)oxytocin, compounds possessing antihormonal properties.


'Cytosome', an addition to the cytoplasmic organelles of Taphrina maculans Butler.


'Early' breast cancer: the Hammersmith trial. An interim report.


'Lysenkoism is not what Canada needs'.

'Lysenkoism is not what Canada needs'.


'Psychoticism' and psychotic illness.

'Psychoticism' and psychotic illness.


'Quality teaching, care depend on research'.


"Controversy in pediatrics".

"Controversy in pediatrics".


"Fish vertebrae," homocystinuria, and sickle cell anemia.


"In-and-out" surgery using general anesthesia. A personal experience.


"Indent" formation and mitotic delay of sea urchin eggs fertilized with X-irradiater sperms.


"Injury pattern" following paroxysmal supraventricular tachycardia in a child.


"Children first!"  Is the slogan in Colombia.


"Ulcerative appendicitis" occurring as a skip lesion in chronic ulcerative colitis; report of a case.


"Staircase" glucose stimulation of insulin secretion in obesity. Measure of beta-cell sensitivity and capacity.


Rat brain hexokinases of two different molecular sizes.


Long-chain cyclic acetals of glycerol: metabolism of the stereomeric 1,3-dioxanes and 1,3-dioxolanes in myelinating rat brain. The metabolism of the stereomeric cyclic glycerol acetals of [1-(14)C]hexadecanal was studied in myelinating rat brain. It was found that the four isomers, cis- and trans-2-pentadecyl-5-hydroxy-1,3-dioxanes and cis- and trans-2-pentadecyl-4-hydroxymethyl-1,3-dioxolanes, were utilized by the tissue at different rates. The acetals were primarily metabolized via a ring-opening mechanism leading to palmitic acid, some of which was subsequently elongated-desaturated. Only the five-membered ring isomers were incorporated as intact acetals into both neutral and polar brain lipids.


Occurrence of cis-5, cis-9-hexacosadienoic and cis-5, cis-9, cis-19-hexacosatrienoic acids in the marine sponge Microciona prolifera.


A lysophospholipase D pathway in the metabolism of ether-linked lipids in brain microsomes.


Physical properties of biological membranes determined by the fluorescence of the calcium ionophore A23187.


Hand efficiency and right-left discrimination.


Individual differences on two measures of incidental learning.


Variability in amylase production among morphological mutants of Aspergillus wentii.


Multidimensional analysis of choice reaction time judgments on pairs of English fricatives.


Evaluation fo membrane filter field monitors for microbiological air sampling. Due to area constraints encountered in assembly and testing areas of spacecraft, the membrane filter field monitor (MF) and the National Aeronautics and Space Administration-accepted Reyniers slit air sampler were compared for recovery of airborne microbial contamination. The intramural air in a microbiological laboratory area and a clean room environment used for the assembly and testing of the Apollo spacecraft was studied. A significantly higher number of microorganisms was recovered by the Reyniers sampler. A high degree of consistency between the two sampling methods was shown by a regression analysis, with a correlation coefficient of 0.93. The MF samplers detected 79% of the concentration measured by the Reyniers slit samplers. The types of microorganisms identified from both sampling methods were similar. Variables in the MF samplers, such as pore size, relative humidity, and flow rates, have been studied, but no effect was noted on recovery. The results show that the MF method could be used to estimate the number and types of microorganisms found in the air.


Effect of orotic acid upon serum bilirubin in newborn infants with erythrocyte G-6-PD deficiency.


A coordinated sickle cell program for economically disadvantaged adolescents.


Mental health and urban life: a study of 850 families.


The course and outcome of pregnancy in women with neuroses.


Diagnostic practice in a psychiatric unit.


Diagnostic differences in psychogeriatric patients in London and New York: United Kingdom-United States diagnostic project.


Psychosomatic aspects of orality and anality.


Beta-glucuronidase in duodenal bile of jaundiced newborn infants treated with phototherapy.


Neonatal death.

Neonatal death.


Changes in the electro-encephalogram in minimal cerebral dysfunction. A controlled study over 8 months.


Long-term follow-up of cyclophosphamide therapy in frequent relapsing minimal lesion nephrotic syndrome.


Barefoot doctors and the medical pyramid.


Letter: Citrulline for Reye's syndrome.

Letter: Citrulline for Reye's syndrome.


A kindred with familial cold urticaria: linkage analysis.


Maturation of nonmyelinated fiber bundles in a strain of dwarf (Snell's) mice.


Host-parasite relationship in echinococcosis. II. Cyst weight, hematologic alterations, and gross changes in the spleen and lymph nodes of C57L mice against graded doses of Echinococcus multilocularis cysts.


Inflammation induced by concanavalin A and other lectins.


The effect of protein malnourishment and caging on growth and behavior of laboratory mice.


Ramazzini Oration. Occupational safety and health. The role of voluntarism.


Case reports. Chronic methyl chloride intoxication in six industrial workers.


Acute short-term treatment in psychiatry.


Letter: Comment on "Fresnel zone plate imaging in nuclear medicine".


A community health program with low achieving adolescents.


Letter: Additional data on hepatic flow studies.


False-positive scintigrams due to malposition of liver.


An economic consideration of portal-systemic anastomoses.


Diagnostic features of clindamycin-associated pseudomembranous colitis.


Implantation in rats treated neonatally with testosterone propionate.


Effect of distillers dried grain with solubles on reproduction and liver fat accumulation in laying hens.


Increased permeability of capillaries to protein during thrombocytopenia. An experimental study in the rabbit.


The effect of acute lateralized brain damage on Halstead's biological intelligence factors.


Natal influences and twin differences.

Natal influences and twin differences.


Size-distance invariance and a distorted texture density gradient.


Rated effectiveness of antidrug letters by drug users and nonusers.


Creative supergroups: group performance as a function of individual performance on brainstorming tasks.


Letter: Overview omissions.

Letter: Overview omissions.


Emergency commitment--a transcultural study.


Letter: The enigma in schizophrenia.

Letter: The enigma in schizophrenia.


An evaluation of evaluations.

An evaluation of evaluations.


Through the viewing tube: videocassette psychiatry.


Letter: Colchicine for familial Mediterranean fever: possible adverse effects.


Defective regulation of inflammatory mediators in Hodgkin's disease. Supernormal levels of chemotactic-factor inactivator.


Mechanisms of immune lysis of the red cells in hereditary erythroblastic multinuclearity with a positive acidified serum test and paroxysmal nocturnal hemoglobinuria. The red cells of patients with hereditary erythroblastic multinuclearity with a positive acidified serum test (HEMPAS), a form of congenital dyserythropoietic anemia, and the cells of patients with paroxysmal nocturnal hemoglobinuria (PNH) are lysed more readily than normal cells by certain antibodies, notably cold agglutinins (anti-I) and complement. With some but not other examples of anti-I, HEMPAS and PNH cells adsorbed more antibody than normal cells. Equal quantities of adsorbed antibody bound equal quantities of the first component of complement (C1) to normal, PNH, and HEMPAS cells. However, for a given quantity of bound antibody and C1, much more of the fourth component of complement (C4) was bound to HEMPAS cells than to normal cells. This resulted in the binding of proportionately larger quantities of the third component of complement (C3) to these cells. The same amount of bound C3 was found on the membranes of normal and HEMPAS cells for a given degree of lysis. Hence, the marked increase in lysis of HEMPAS cells is due to the increased adsorption of antibody and/or increased binding of C4.PNH cells bound the same amount of C4 per bound C1 as normal cells but bound more C3 than normal cells. However, the mean concentration of C3 on the membrane of PNH cells was one-third to one-fifth that on normal cells for a given degree of lysis. Hence, the increased lysis of PNH cells is due to the increased binding of C3 and increased hemolytic effectiveness of the bound C3.


Impaired lymphocyte function in aged humans. The response of lymphocytes from young and old persons to phytohemagglutinin, pokeweed mitogen, or allogeneic lymphocytes has been measured. Lymphocytes from old persons incorporated significantly less tritiated thymidine as compared with lymphocytes from young persons when cultured with plant mitogens or allogeneic cells. The difference in observed lymphocyte reactivity could not be attributed to differences in culture conditions required for maximal transformation of lymphocytes from old or young subjects. The same percentage of thymus-derived and bone marrow-derived lymphocytes was found in the blood from old and young persons. The relationship of these findings to the decline of immunologic competence with age is discussed.


Double-antibody radioimmunoassay for parathyroid hormone.


The effect of iron content on the behavior of human ferritin in an inhibition-type radioimmunoassay.


Cholesterol synthesis by cultured fibroblasts: decreased feedback inhibition in familial hypercholesterolemia.


Mechanism of action of naturally occurring proteinase inhibitors. Studies with anhydrotrypsin and anhydrochymotrypsin purified by affinity chromatography.


Affinity labeling of a distinctive lysyl residue within the second hypervariable region of gamma 2 chain of guinea pig anti-p-azobenzenearsonate antibody.


Surface glycoproteins of mouse L cells.

Surface glycoproteins of mouse L cells.


Effect of recent antigen priming on adoptive immune responses. II. Specific unresponsiveness of circulating lymphocytes from mice primed with heterologous erythrocytes. When thoracic duct lymphocytes (TDL) or mesenteric lymph node (MLN) cells from mice primed 1 day before with either sheep erythrocytes (SRC) or horse erythrocytes (HRC) were transferred together with both SRC and HRC to irradiated mice, antibody responses measured 7 days later could not be detected to the priming antigen but were high to the other antigen. Furthermore, this unresponsiveness of TDL and MLN to the priming antigen could not be abrogated by delaying antigen challenge of the transferred cells for 1-2 wk. Previous work had shown that short-term priming with antigen also induced specific unresponsiveness in spleen cells on adoptive transfer. Unresponsiveness in these cells, however, was only of temporary duration, full recovery in the reactivity of the cells being observed when challenge with the priming antigen on transfer was delayed for 5 or more days. Since the present work showed that such recovery from initial unresponsiveness on transfer was unique to spleen cells and did not apply to TDL or MLN, it appeared that different mechanisms were responsible for the unresponsiveness in the three populations. It is proposed that the unresponsiveness detected in TDL and MLN cells in the present study resulted from a deficiency of antigen-reactive cells, these cells having been recruited to the spleen, i.e., a region of antigen concentration. This concept of antigen-induced selective recruitment of circulating lymphocytes was supported by evidence that (51)Cr-labeled heterologous erythrocytes indeed localized largely in the spleen after intravenous injection but not in MLN.


General anesthesia and myocardial infarction.


Quantitative aspects of follicular development in the untreated and PMS-treated cyclic hamster.


Fine structure of the agranular cytoplasmic tubules in the lamprey chloride cells.


Atopic allergy in Japanese subjects: studies primarily with radioallergosorbent test.


Lead pica produced in rats.

Lead pica produced in rats. Weanling rats eating a low calcium diet voluntarily ingested lead acetate solutions in much greater proportions than did iron-deficient or control weanlings. This increased ingestion occurred even with high concentrations of lead acetate which normal weanlings found extremely aversive. Chronic injections of lead acetate into weanlings did not change lead ingestion, indicating an absence of behavioral regulation of body lead levels. Female lead-injected weanlings did show a significant increase in calcium ingestion. Calcium deficiency may be one component of lead pica.


Serotyping of Chlamydia. I. Isolates of ovine origin. Eight chlamydial isolates of ovine origin were tested in a plaque reduction system using homologous and heterologous rooster antisera. The eight isolates could be separated into two separate immunotypes. Type 1 included isolates associated with ovine abortion and one agent recovered from the feces of an apparently normal sheep. Type 2 isolates were associated with polyarthritis and conjunctivitis. These two serotypes were not cross-reactive with several chlamydiae of avian origin. Further application of the plaque reduction test may provide a useful means of typing chlamydiae.


Norepinephrine uptake into cerebral cortical synaptosomes after one fight or electroconvulsive shock. Membrane affinity for the neurotransmitter norepinephrine is rapidly but reversibly decreased in nerve terminals of the cerebral cortex by intense nervous stimulation. This should adaptively facilitate alerting during acute emergency and stress. The Michaelis constant (K(m)) for the high-affinity active uptake of norepinephrine into crude synaptosome-rich homogenates of the cerebral cortices of mice was increased 68 percent after 15 minutes of intense fighting and 110 percent 5 minutes after a single electroconvulsive shock. These changes were no longer evident 18 to 20 hours later.


Serum unconjugated estriol in normal and abnormal pregnancy.


Plasma luteinizing hormone, follicle-stimulating hormone and prolactin during pregnancy in the mouse.


On the mechanism of the onset of puberty: identification and pattern of 5 alpha-androstane-3 beta, 17 beta-diol and its 3 alpha epimer in peripheral blood of immature female rats.


Sex-dependent changes in rat retinal hydroxyindole-O-methyl transferase.


Reversal of myocardial ischemia by arterialization of the coronary vein.


Tryptophan biosynthesis from indole-3-acetic acid by anaerobic bacteria from the rumen. Microbes in ruminal contents incorporated (14)C into cells when they were incubated in vitro in the presence of [(14)C]carboxyl-labeled indole-3-acetic acid (IAA). Most of the cellular (14)C was found to be in tryptophan from the protein fractions of the cells. Pure cultures of several important ruminal species did not incorporate labeled IAA, but all four strains of Ruminococcus albus tested utilized IAA for tryptophan synthesis. R. albus did not incorporate (14)C into tryptophan during growth in medium containing either labeled serine or labeled shikimic acid. The mechanism of tryptophan biosynthesis from IAA is not known but appears to be different from any described biosynthetic pathway. We propose that a reductive carboxylation, perhaps involving a low-potential electron donor such as ferredoxin, is involved.


In vitro platelet phagocytosis by splenic leukocytes in idiopathic thrombocytopenic purpura.


Editorial: Platelet-phagocyte interactions in idiopathic thrombocytopenic purpura.


Lacunae in erythrocyte monolayers induced by adherent leukocytes.


Reversal by narcotic antagonist of a narcotic action elicited by a conditional stimulus.


Rubella surveillance and immunization among college women.


The diagnosis of iron deficiency in pregnancy.


Osteogenesis imperfecta diagnosed in utero in conjunction with Rh sensitization.


Ciguatera poisoning: a common source outbreak.


Letter: Delayed cutaneous hypersensitivity reactions to membrane extracts of carcinomatous cells of the cervix uteri.


Bacterial invasion of the urinary tract by way of the vaginal route in the rat.


Chymotrypsinogen in the intestine of rats fed soybean trypsin inhibitor and its inability to suppress pancreatic enzyme secretions.


Working together for the public interest.


Androgen-induced sexual differentiation of the brain is blocked by inhibitors of DNA and RNA synthesis.


Use of turkey erythrocytes for vaccinia virus hemagglutination and hemagglutination-inhibition tests.


Investigations of variables affecting rheumatoid factor reactivity and tests. I. Influence of amboceptor concentration, ratio of IgG to IgM, and source, in the sheep-cell agglutination test.


Noradrenaline binding and the search for catecholamine receptors.


The development of a curriculum in drug dependency.


The student psychiatric ward: an innovative teaching approach for medical undergraduates.


Letter: Health care in Jamaica.

Letter: Health care in Jamaica.


Oncology education and the preclinical medical student.


Prolonged rupture of membranes associated with a decreased incidence of respiratory distress syndrome.


Detection of human lymphocytes bearing complement receptors after in vitro modification with mercaptoethanol.


Effect of pregnancy on the reactions of lymphocytes with cytotoxic antisera.


The major histocompatibility system (H-1) alleles of some British rat strains.


Microtubule surface lattice and subunit structure and observations on reassembly. Neuronal microtubules have been reassembled from brain tissue homogenates and purified. In reassembly from purified preparations, one of the first structures formed was a flat sheet, consisting of up to 13 longitudinal filaments, which was identified as an incomplete microtubule wall. Electron micrographs of these flat sheets and intact microtubules were analyzed by optical diffraction, and the surface lattice on which the subunits are arranged was determined to be a 13 filament, 3-start helix. A similar, and probably identical, lattice was found for outer-doublet microtubules. Finally, a 2-D image of the structure and arrangement of the microtubule subunits was obtained by processing selected images with a computer filtering and averaging system. The 40 x 50 A morphological subunit, which has previously been seen only as a globular particle and identified as the 55,000-dalton tubulin monomer, is seen in this higher resolution reconstructed image to be elongated, and split symmetrically by a longitudinal cleft into two lobes.


Electron microscopy of the Mo-Fe protein from Azotobacter nitrogenase.


5-Hydroxy-5-phenyllevulinic acid. A novel microbial metabolite with analgesic activity.


Stimulation by insulin of RNA synthesis in chick fibroblasts. After the addition of insulin to monolayers of chick fibroblasts previously incubated in serum-free medium, the rates of protein and RNA synthesis increase continuously during the first 8-10 h. Little stimulation of DNA synthesis or mitosis results with the addition of insulin alone in contrast to the addition of fresh serum which stimulates both markedly. The stimulation in RNA synthesis does not result from expansion of the nucleotide pool but is correlated with increases in RNA polymerase activity. All major classes of RNA are stimulated; processing of preribosomal RNA to 28S and 18S and the association of this mature RNA with ribosomes appear to occur normally. The kinetics of stimulation of 5S RNA differ from those of the synthesis of 4S and of ribosomal RNA. Insulin and serum appear to affect the synthesis or stability of certain transcripts differentially.


An experimental study of the mechanism of action of ketamine on the central nervous system.


Occupational health nursing participation in the development of part-time nursing services to small industries.


Nursing education and the occupational health setting.


Occupational health and the Health Maintenance Organization.


Problem drinking.

Problem drinking.


Progesterone metabolism by proliferative and secretory human endometrium.


A radioimmunoassay for plasma unconjugated estrogens in normal pregnancy.


Echinococcus discovered during pregnancy.


Incomplete testicular feminization syndrome with puberal virilization.


The lumbar lymphatics: a lymphographic study.


Le Dain Commission of Inquiry into the Non-Medical Use of Drugs tables fourth and final report.


A note on growth patterns of epithelial tumor cells in primary culture.


Chromosomal heterogeneity in the RAG and MSWBS mouse tumor cell lines.


Glycylglycylglycine as a synthetic standard for serum protein determination.


Sources of error in spectrophotometric measurement of aspartate aminotransferase and alanine aminotransferase activities in serum.


Immunopathologic uveitis in the mouse due to lymphocytic choriomeningitis virus.


Editorial: Laser treatment for glaucoma.


A quantitative assay for concanavalin A-mediated cell agglutination.


Selective sulfenylation of tryptophan residues in alpha-lactalbumin of bovine milk.


Changes in neutral lipid constituents during differentiation of the cellular slime mold, Dictyostelium discoideum.


Purification and characterization of the molybdenum-iron protein component of nitrogenase from soybean nodule bacteroids.


Study of the incidence of hypospadias in Rochester, Minnesota, 1940-1970, and a case-control comparison of possible etiologic factors. A community-wide (Rochester, Minnesota), case-control study of the incidence severity, trends, and possible etiologic factors, both environmental and genetic, of hypospadias, between 1940-1970, is presented.  Of 13,776 live male births, 113 cases 8.2/1000) of hypospadias were found.  There was no significant variation in the rates by 5-year periods and no cyclic pattern noted.  In 7 instances, 2 brothers were affected, and in 4 families, the father was also affected.  There was no difference between index and control mothers with respect to drug usage.  Hydroxyprogesterone caproate, i.m., had been given to 4 index mothers and 1 control mother, 3 index mothers had received thyroid therapy during the first trimester, 1 index mother and 4 control mothers had received meclizine, 4 control  mothers had received estrogen, and 9 index mothers and 5 control mothers had taken oral contraceptives (combination) within 9 months of their last menstrual period.  There was a larger proportion of testicular anomalies which might have adversely affected spermatogenesis and semen quality among index fathers than among control fathers.  These data suggest a multifactorial etiology of hypospadias fitting a polygenic model.


Aeromonas hydrophila wound infection.

Aeromonas hydrophila wound infection.


Immunosuppressive therapy of chronic idiopathic thrombocytopenic purpura.


Evolution of an anthelmintic: vincofos.

Evolution of an anthelmintic: vincofos.


Luteolysis, luteostasis, and the effect of prostaglandin F2 alpha in cows after endometrial irritation.


The effect of trypsin on nuclease-resistant chromatin fragments.


Physicochemical properties of bovine mammary fatty acid synthetase.


Porcine intestinal calcium-binding protein. Molecular properties and the effect of binding calcium ions.


Multiple forms of deoxyribonucleic acid-dependent ribonucleic acid polymerase in Xenopus laevis. Isolation and partial characterization.


Multiple forms of deoxyribonucleic acid-dependent ribonucleic acid polymerase in Xenopus laevis. Levels of activity during oocyte and embryonic development.


Chemical studies of histone acetylation. Substrate specificity of a histone deacetylase from calf thymus nuclei.


Familial 78XX male pseudohermaphroditism in three dogs.


The needs of the occupational health nurse in relation to arthritis.


Perinatal complications in the history of asthmatic children.


Neurologic worm diseases. 3.

Neurologic worm diseases. 3.


PSROs--implications for hospitals.

PSROs--implications for hospitals.


Health care in the 1980s.

Health care in the 1980s.


Autoradiographic studies on (3H)oestradiol localization in the blastocysts and uterus of rats during delayed implantation. The localization of tritiated estradiol by autoradiographic techniques to provide evidence for the direct action of estrogen on the blastocysts was investigated.  There was no retention or subcellular radiographic activity demonstrated in flushed-out blastocysts as well as blastocysts in situ.  Uterine stromal, glandular and muscle cells showed characteristic retention of the steroid.  Several mechanisms for this are proposed, including the transient binding of estradiol which could not be detected as late as 5 minutes after instillation.


Effects of cortisol on immune cytolysis.


Nature of the pre-penetration contact interactions between hamster gametes in vitro.


Collaborative study of the effects of human growth hormone in growth hormone deficiency. II. Development and significance of antibodies to human growth hormone during the first year of therapy.


Anesthesia for uncomplicated obstetric delivery.


The industrial nurse's role in occupational safety.


Women's attitudes regarding breast cancer.


Cytochrome P-450cam. III. Removal and replacement of ferriprotoporphyrin IX.


Gas exchange in the immature lung. II. Method of estimation and maturity.


Evaluation of micro complement fixation tests for antibodies against group A streptococcal M and M-associated antigens in rabbit and human sera. Variables involved in micro complement fixation (CF) tests for type-specific (TSM) and non-type-specific (NTSM) antibodies against preparations of streptococcal M protein were studied. Sera of rabbits immunized with purified M protein which contained high titers of anti-TSM and low titers of anti-NTSM antibodies reacted type specifically in the CF tests when relatively low concentrations of M protein were employed. In contrast to these artificially induced TSM antibody responses, the sera of rheumatic fever patients demonstrated elevated CF antibody titers (1:80 to 1:320) in the presence of both high and low concentrations of M antigen. The CF test cannot distinguish TSM and NTSM antibodies when the latter predominate. The CF test can be employed with confidence for the detection of NTSM antibodies, provided that the test sera are known to lack type-specific antibodies against the M protein used in the test.


Genetic differences in the ventilatory response to inhaled CO2.


Purification on renografin density gradients of Chlamydia trachomatis grown in the yolk sac of eggs. Chlamydia trachomatis grown in the yolk sac of embryonated eggs was purified by centrifugation on continuous isopycnic Renografin density gradients. A band of chlamydial particles with a buoyant density of 1.20 contained 70% of the starting particles, and electron microscopy revealed the virtual absence of contaminating egg material. Centrifugation on Renografin gradients caused only a moderate decrease in infectivity. For large-scale purification, infected yolk sac was centrifuged through Renografin solutions, resulting in greater than 60% recovery of starting chlamydial particles, but less than 1% recovery of the dry weight and protein.


Nephelometric methods for the determination of urinary albumin, transferrin and alpha-2 macroglobulin.


Use of Japanese quail embryo fibroblast cells for propagation and assay of turkey herpesvirus FC-126. Japanese quail (Coturnix coturnix japonica) fibroblast cell culture monolayers were found to provide a very satisfactory system in which to propagate and assay turkey herpesvirus FC-126, which is used for production of Marek's disease vaccine. Japanese quail cells were more sensitive than duck cells and of approximately equal sensitivity to chicken cells. Foci of infection developed rapidly and uniformly, were of larger size, and were more easily discernible in quail cells than in chicken cells.


L-Asparaginase production by the rumen anaerobe Vibrio succinogenes. The rumen anaerobe Vibrio succinogenes possesses a constitutive L-asparaginase. The amount of enzyme produced is affected by the compound supplied to the organism to generate the fumaric acid it requires as a terminal electron acceptor. When nitrate is provided as the terminal electron acceptor, the amount of enzyme produced is affected by the compound provided to satisfy the nutritional requirement of the organism for succinic acid. Specific activities of up to 8.4 IU/mg of protein in cell-free extracts have been obtained. This specific activity is higher than has been previously reported for any organism. The enzyme has an apparent K(m) of 1.7 x 10(-5) M and low activity towards L-glutamine when assayed at pH 8.5.


Inheritance of learning ability in mice: a diallel-environmental analysis.


Echocardiographic manifestations of discrete subaortic stenosis.


Teenage pregnancy.

Teenage pregnancy.


Partial specific volumes and interactions with solvent components of proteins in guanidine hydrochloride.


Influence of temperature on the intrinsic viscosities of proteins in random coil conformation.


Phleomycin-induced cleavage of deoxyribonucleic acid.


Thermodynamics of hapten binding to MOPC 315 and MOPC 460 mouse myeloma proteins.


On the structure of ovotransferrin. II. Isolation and characterization of a specific iron-binding fragment after cyanogen bromide cleavage.


On the sturcture of ovotransferrin. 3. Nitration of iron--ovotransferrin and distribution of tyrosines involved in iron-binding activity.


Sounding board: Washington health ball.

Sounding board: Washington health ball.


Letter: Measles-associated optic neuritis in man and canine distemper.


Clinical and EEG manifestations of an unusual aphasic syndrome in children.


Discriminative aspects of visual hemi-inattention.


Cyclic oculomotor paralysis.

Cyclic oculomotor paralysis.


Amantadine hydrochloride: alteration in peripheral circulation.


A new familial arthrogryposis without weakness.


Propranolol treatment of essential tremor.


Movement disorder as a manifestation of diphenylhydantoin intoxication.


Cerebral infarction due to fungal embolus. A complication of Aspergillus infection on an aortic valve prosthesis.


Letter: Quotation on drug abuse.

Letter: Quotation on drug abuse.


Malignant testicular tumours.

Malignant testicular tumours. A series of 71 patients with malignant testicular tumours treated primarily by orchiectomy and irradiation is reviewed with respect to pathological and clinical features and modes of treatment.The three-year crude survival rate in 36 patients with seminoma was 86% and in 24 patients with carcinoma it was 41.7%. There were no survivors among patients with choriocarcinoma. Our results are comparable with those of other series.A prospective study is proposed of the value of irradiation and subsequent limited lymph node dissection following orchiectomy in cases of carcinoma of the testis.


Human botulism in Canada (1919-1973).

Human botulism in Canada (1919-1973). Since 1919, in Canada, 62 authenticated outbreaks of human botulism have affected 181 persons, with 83 deaths, a fatality rate of 46%. Among these, 41 outbreaks were bacteriologically determined (31 in one laboratory) as six type A, four type B, one both A and B, and 30 type E. About two thirds of the total outbreaks, cases and deaths involved Eskimos and Pacific coast Indians consuming raw marine mammal products and salmon eggs, respectively. Other parts of Canada recorded seven occurrences due to miscellaneous vehicles, three being type B. Since January 1961 there have been 38 outbreaks, involving 94 cases with 33 deaths. These include 18 outbreaks among Eskimos, affecting 51 persons (of whom 24 died) in Labrador, southern Baffin Island, northern Quebec, and the Mackenzie area. Also, putrid salmon eggs caused 15 outbreaks among Pacific coast Indians, totalling 35 cases, of whom only six died, the low fatality rate reflecting the introduction of type E botulinus antitoxin during 1961.


Mechanism of sperm transport in pig oviducts.


Maintenance of pregnancy and tissue carbohydrate levels by vitamin C in rats on restricted diets.


Subcutaneous mycotic infection of a white-tailed deer.


A preliminary report on potentially pathogenic microbiological agents recently isolated from pinnipeds.


Genetics of growth in axenic medium of the cellular slime mould Dictyostelium discoideum.


Long-range motor performance changes in levodopa-treated patients with Parkinson's disease.


Deanol in the treatment of levodopa-induced dyskinesias.


Angiographic sign of good prognosis for hemiplegia in hypertensive intracerebral hemorrhage.


Late onset hereditary distal myopathy.

Late onset hereditary distal myopathy.


Increased paralysis induced by guanidine in motor neuron disease.


Cerebrospinal fluid resistance and compliance in subacutely hydrocephalic cats.


Electrophysiologic analysis of epileptic beagles.


Melanosis of the dentate nucleus.

Melanosis of the dentate nucleus.


Infantile Gaucher's disease: A biochemical study.


Effect of hemispheral lesions on the contralateral blink reflex.


Inhibition of cerebral protein synthesis by epileptic seizures without motor manifestations.


Effects of sympatholytic agents on CNS regeneration, cystic necrosis after spinal cord transection.


Insulin half-life and utilization in normal pregnancy.


Oral prostaglandin E2 for induction of labor at term.


Fulminant herpesvirus hominis hepatitis during pregnancy.


The association of hydatidiform mole and trisomy 2.


Slow cortical potential consequences of elctroconvulsive shock in rats.


Receptivity in female hamsters following neonatal testosterone, testosterone propionate, and MER-25.


Isolation stress and volitional ethanol consumption in the rat.


Clinical usefulness of beta-adrenergic blocking agents.


Prolactin-secreting pituitary microadenomas: roentgenologic diagnosis.


Observations on the practice of anesthesia in the People's Republic of China.


Patient safety: prevention and prompt recognition of regurgitation and aspiration.


Evaluation of the cardiopulmonary safety and effects of lorazepam as a premedicant.


Letter: Noise-free electrocardiographic data during electrosurgical procedures.


The practice of acupuncture in China.

The practice of acupuncture in China.


The use of naloxone to antagonize large doses of opiates administered during nitrous oxide anesthesia.


Letter: Mouth-to-tube resuscitation of the neonate.


Lupus meningitis. Antibody to deoxyribonucleic acid (DNA) and DNA:anti-DNA complexes in cerebrospinal fluid.


Letter: Closed circuit anesthesia.

Letter: Closed circuit anesthesia.


Dietary amino acids: effect of depletion and recovery on protein synthesis in vitro in rat skeletal muscle and liver.


Legislation regulating emergency medical care.


Relative contributions of ovarian and adrenal progesterone to the timing of heat in the 4-day cyclic rat.


Intraoperative effects of Ringer's solutions incorporating bicarbonate precursors.


A clinical evaluation of pyridostigmine bromide in the reversal of pancuronium.


Habituation of fear: effects of intensity and stimulus order.


Influence of 2-Br-alpha-ergocryptine on serum levels of prolactin and the estrous cycle in sheep.


Thoughts about medical writing. XXI. The delicate question of plagiary.


Cardiovascular effects of high dosages of fentanyl, meperdine, and naloxone in dogs.


Endotracheal tube holder.

Endotracheal tube holder.


Angioblastic meningioma (hemangiopericytoma) of the cerebellar fossa with metastases to the temporal bone and the lumbar spine.


Small artery and vein response to ketamine HCl in the bat wing.


Pancuronium bromide as the sole muscle relaxant for major surgery.


Side effects after ketamine anesthesia: transient blindness.


Cerebellar hemangioblastoma with an enlarged tentorial artery.


Broken disposable needle during an axillary approach to block the brachial plexus.


Echocardiographic findings in discrete subvalvular aortic stenosis.


Determination of total affinity constant for heterogeneous hapten-antibody interactions.


Immunochemistry of sperm-whale myoglobin. XVI. Accurate delineation of the single region in sequence 1-55 by immunochemical studies of synthetic peptides. Some conclusions concerning antigenic structures of proteins.


Left ventricular subendocardial ischemia in severe valvar and supravalvar aortic stenosis. A common mechanism.


Effects of early malnutrition on Swiss white mice: avoidance learning after rearing in large litters.


Safe use of methoxyflurane "analgesia" during abdominal surgery.


A clinical comparison of pancuronium and tubocurarine for cesarean section anesthesia.


An objective technique for recording shock-induced aggression in unrestrained pairs of rats. Pairs of rats received grid shock in a conical enclosure. Each shock elicited the stereotyped fighting posture. An omnidirectional pole, which was always between the rats, was accidentally operated whenever the rats fought. The number of pole hits, and the number of observed fight bouts, increased with current intensity. The number of pole hits served as a reliable and objective measure of shock-induced fighting in rats.


Chemical contamination of umbilical catheter blood samples by infusates.


Authoritarian and accommodative child-rearing styles: their relationships with the behavior patterns of 2-year-old children and with other variables.


Perineal vesicostomy: an alternative to cystectomy in male patients with a permanently defunctionalized bladder.


Abnormalities of cell-mediated immunocompetence in genitourinary cancer.


Newcastle disease virus-specific RNA: polyacrylamide gel analysis of single-stranded RNA and hybrid duplexes. Newcastle disease virus-specific [(3)H]uridine-labeled 18S RNA was resolved by polyacrylamide gel electrophoresis into several components with molecular weights from 450,000 to 840,000. The analysis of 35 and 24S virus-specific RNA also revealed several components in each sedimentational class. The conversion of 18S RNA into double-stranded form by hybridization with an excess of unlabeled virion RNA improved the resolution in polyacrylamide gels and revealed at least six distinct components. The same six classes of hybrid duplexes were revealed when (32)P-labeled 50S virion RNA was hybridized with an excess of 18S RNA. The applicability of polyacrylamide gel electrophoresis of hybrid duplexes to the analysis of viral genome structure is discussed.


Inhibition of virus release by antibodies to surface antigens of influenza viruses. When influenza virus was mixed with antisera to its surface subunits before inoculation of cell cultures, anti-hemagglutinin antibodies neutralized infectivity but anti-neuraminidase did not. When the antisera were added after infection of cell cultures, anti-hemagglutinin and anti-neuraminidase antibodies were equally effective in reducing virus titers in culture fluids. Decreased virus titers were not due to interference of antibody with assay and were not accompanied by a reduction in the synthesis of hemagglutinin and neuraminidase subunits. Both antisera also effectively prevented in vitro virus spread. Inhibition of virus release by neuraminidase antibody appeared unrelated to its antienzyme property. Hydrolysis of N-acetyl neuraminic acid residues of infected host cells proceeded unimpaired in the presence of subunit antisera. Anti-hemagglutinin and anti-neuraminidase antibodies may act to prevent virus release by binding newly formed virus subunits to each other and to anti-genically altered cell membranes.


Replication of reticuloendotheliosis viruses in cell culture: acute infection. Replication of reticuloendotheliosis viruses (REV) in cultures of chicken and duck fibroblasts leads to some cell death soon after infection. This cell killing was used to develop a plaque assay for Trager duck spleen necrosis virus (TDSNV) on duck embryo fibroblasts. A normal replicative cell cycle was required for normal virus production and the development of cytopathic effects in chicken cells exposed to TDSNV. The latent period was about two days. Stationary chicken embryo fibroblasts could be infected by REV; DNA synthesis was required, but protein synthesis was not.


Replication process of the parvovirus H-1. II. Isolation and characterization of H-1 replicative form DNA. The Parvovirus H-1 replicates autonomously in hamster embryo cells. A DNA synthetic event, called HA-DNA synthesis, upon which subsequent viral RNA and viral hemagglutinin synthesis is dependent, is initiated in late S phase of the infected cell (18). It was postulated that HA-DNA represents parental viral replicative form DNA (RF DNA). This study describes the isolation and characterization of H-1 RF DNA as part of the continuing study of the mechanisms and control of DNA replication in the eukaryotic cell. The H-1 RF DNA is a linear duplex molecule containing the viral strand and its complement. The complementary strands of the RF DNA have been separated by equilibrium density gradient centrifugation. The RF DNA has a buoyant density of 1.705 in neutral CsCl and an estimated guanine plus cytosine (GC) content of 45.9%. It has a sedimentation coefficient of 17S. The calculated molecular weight of 3.7 x 10(6) is twice that of the single-stranded virion DNA. H-1 virions contain DNA that is homogeneous and free of complementary strands.


Age-dependent cannibalism in a colony of albino rats.


Histones stimulate polyribonucleotide-directed polydeoxyribonucleotide synthesis by murine leukemia virus. The rate of homoribopolymer-directed DNA synthesis by detergent-disrupted Moloney murine leukemia virus can be stimulated or inhibited by histone, depending on the ratio of histone to template. Of the fractions which can be separated from the whole histone, f1 causes both the greatest stimulation and the greatest inhibition. The effect of histone f1 is qualitatively similar whether the template is polyadenylate (poly A), polycytidylate, or polyuridylate, but the stimulation is greatest with poly A. The pattern of stimulation and inhibition differs, however, for a different polymerase; the DNA polymerase of Micrococcus luteus is inhibited by histone concentrations which stimulate the viral enzyme and stimulated by concentrations which inhibit the viral enzyme. For the viral enzyme, the optimum histone concentration is unaffected by changes in the virus or primer concentration; but it varies in proportion to the template concentration, suggesting that histone acts by combining stoichiometrically with the template. These data raise the possibility that a histone-like protein may participate in the synthesis of the provirus of RNA tumor viruses.


Temperature-sensitive virus from Aedes albopictus cells chronically infected with Sindbis virus. Cultures of Aedes albopictus cells persistently infected with wild-type Sindbis virus (SV-W) give rise to small plaque-forming mutants which are also temperature sensitive. These mutants, designated SV-C, are neutralized by antiserum produced against SV-W. Mutant ts clones were isolated from SV-C by plaque purification. After serial undiluted passage in BHK or mosquito cells, each of the clones gave rise to ts(+) revertants which, however, remained mutant with respect to plaque morphology. Nineteen of 20 clones derived from SV-C were RNA(+), and one was RNA(-) (SV-C-2). The RNA synthesizing activity, once induced in infected cells by SV-C-2, was stable at the nonpermissive temperature (39.5 C). All clones derived from SV-C were inactivated at 60 C much more quickly than was SV-W. It was not possible to demonstrate complementation between any of the SV-C clones.


Occurrence of oestrus in the Mongolian gerbil after pairing with a male.


Lipectomy in obese hyperglycemic mice (ob-ob).


Letter: Diagnosis of neonatal jaundice.

Letter: Diagnosis of neonatal jaundice.


Government continues efforts to control hospital admissions.


Renal and skeletal lesions in experimental cadmium poisoning: histological and biochemical approaches.


Studies on the mechanism of action of 5-aziridinyl-2, 4-dinitrobenzamide in tumor cells.


Herpesvirus type 2-induced thymidine kinase and carcinoma of the cervix.


Stereochemical characteristics of the folate-antifolate transport mechanism in L1210 Leukemia cells.


Inhibition of DNA repair by the 1,3-bis(2-chloroethyl)-1-nitrosourea breakdown product, 2-chloroethyl isocyanate.


Comparison of synthetic and natural porcine secretins on human pancreatic secretion.


Properdin factor D: characterization of its active site and isolation of the precursor form. The activity of properdin factor D was measured by the generation of the hemolytically active cellular intermediate, EAC43B(D), bearing the C3b-dependent alternate pathway C3 convertase. Treatment of factor D with DFP prevented formation of EAC43B(D); thus, a serine esterase is essential for the generation of the alternate pathway C3 convertase, a situation analogous to the role of C1 in the formation of the classical C3 convertase, C42. The definition of factor D as a serine esterase prompted a search for its proenzyme form, and resulted in the chromatographic isolation from plasma of a single peak of trypsin-inducible factor D activity, distinct from activated factor D. Analytical gel filtration indicated an apparent mol wt of 25,000. This protein from which trypsin elaborated factor D activity, as assessed by the formation of EAC43B(D), the generation of the CoVF-dependent C3 convertase, and the cleavage of factor B in the presence of C3b, was designated "precursor factor D." The DFP resistance of precursor factor D, and the susceptibility of its trypsin-activated form to inactivation by DFP is analogous to the behavior of other plasma serine esterases, including C1.


Humoral immunostimulation. I. Increased uptake of (125I)iododeoxyuridine and (3H)thymidine into TNP-cells treated with anti-TNP antibody. Interaction of microgram quantities of highly purified rabbit anti-TNP antibodies with TNP-substituted HeLa, HEp-2, and L cells caused an intense stimulation of radioactive nucleoside ([(125)I]UdR and [(3)H]TdR) uptake which was maximal 24-72 h after exposure of cells to antibody. The stimulation of nucleoside uptake and presumaly DNA synthesis was shown to be immuno logically mediated because unsubstituted cells were not stimulated by anti-TNP antibody, normal rabbit gamma globulin did not stimulate TNP-cells, and a hapten inhibitor, epsilon-DNP-lysine, prevented the stimulation of TNP-cells by anti-TNP antibody. These findings demonstrate that interaction of antibody with cell surface antigen can alter cell membrane transport, and possibly can enhance cell growth.


Specific heterologous enhancement of immune responses. VI. Partial purification of a nonspecific enhancing factor from supernates of allogeneically stimulated human lymphocyte cell lines. The mixing of two histoincompatible human lymphocyte cell lines generated the release of a soluble factor which was capable of nonspecifically enhancing the in vitro immune response of normal mouse spleen cells against sheep erythrocytes. When active supernates were subjected to exclusion chromatography on Sephadex G-150 and BioGel P-150, the active principle eluted with molecules of approximately 35,000 mol wt. Column aliquots from similarly treated supernates from independent cultures of each lymphoid cell line were inactive. The human enhancing factor was concentrated and purified by ammonium sulfate fractionation, followed by Sephadex gel filtration and polyacrylamide gel electrophoresis.


Appearance of a folate binder in leukocytes and serum of women who are pregnant or taking oral contraceptives.


Aflatoxin and trichothecene toxins: skin tumor induction and synergistic acute toxicity in white mice.


Inhibition of gross leukemia virus-induced plaque formation in XC cells by 3-deazauridine.


Unusual occurrence of endometrial sarcomas in hybrid mice.


Altered amino acid concentrations accompanying suppression of malignancy of mouse melanoma cells by 5-bromodeoxyuridine.


Direct action of estradiol on rat mammary tumors. This study reports a direct action of 17-beta-estradiol on protein synthesis by 7, 12-dimethylbenz (alpha) anthracene (DMBA) induced rat mammary tumors.  Sprague-Dawley female rats were given 20 mg of DMBA in sesame oil by gastric tube.  Mammary tumors developed.  When tumors reached 1.5 to 2 cm in diameter, animals were ovariectomized.  At 4-7 days later animals were killed and tumors removed.  Microscopic examination confirmed the tumors to be carcinoma of adenoid cystic variety with regressive changes.  14 tumors from 14 animals were found suitable for study.  In vitro treatment with 17-beta-estradiol gave rise to a 7% increase in the rate of 3H-leucine incorporation, expressed as dpm/mg of TCA-insoluble protein.  This increase was considered statistically significant (p.001).  A large variation among different tumors was noted.  Also results varied with differences in time after ovariectomy.  Under the same incubation conditions the same percentage of increase in the rate of 3H-leucine incorporation had been observed in the study of the effect of estrogen on the uterus of ovariectomized rats.  In other mammalian tissues studied those containing high levels of estrogen receptor were able to respond to direct stimulation of estrogen.  It was concluded that estrogen directly stimulates protein synthesis in the mammary tumors.  This supports the view that these tumors are estrogen responsive tissues.


Tumor cell-surface organization: differences between two TA3 sublines.


Phosphorylated proteins of Sindbis virus. The capsid and two membrane proteins of Sindbis virus, grown in chicken cells, contain 0.03 to 0.1 mol of phosphate per mol of protein.


The preparation of a chemically cross-linked complex of the basic pancreatic trypsin inhibitor with trypsin.


Interactions of aromatic residues of proteins with nucleic acids. I. Proton magnetic resonance studies of the binding of tryptophan-containing peptides to poly(adenylic acid) and deoxyribonucleic acid.


Interactions of aromatic residues of proteins with nucleic acid. II. Proton magnetic resonance studies of the binding of tyramine and tyrosine-containing peptides to poly(adenylic acid) and deoxyribonucleic acid.


Computation of structures of homologous proteins. Alpha-lactalbumin from lysozyme.


Effects of detergents and high pressures upon the metarhodopsin I--metarhodopsin II equilibrium.


Prostaglandin-mediated action of intrauterine devices: F-prostaglandins in the uterine horns of pregnant rabbits with unilateral intrauterine devices.


Copper as a dissociating agent of liver and endometrial polysomes. In an effort to explain the mechanism of the increased efficacy of the copper-releasing IUD, the in vitro action of copper ions on the polysome patterns of rabbit endometrium and liver was studied.  The uterine horns were extracted from female rabbits with prior sexual experience.  The horns were sliced longitudinally, and the endometrium carefully scraped off with a curette.  For each experiment the scrapings from 4 to 6 cornua were pooled, suspended in buffer A (.14 M sucrose containing .05 M Tris-hydrochloride, .05 M potassium chloride, .005 M magnesium chloride, 200 mcg/ml heparin and brought to pH 7.6), and  divided into 5 equal fractions.  To 4 of the fractions, copper (as copper chloride) was added to reach final concentrations of .15, 13, 1, and 1.5 mM, respectively.  The fifth fraction served as a control with no copper added.  The liver was extracted, homognized in buffer A, and treated in the same way as the endometrium.  The homogenates were further adjusted, treated, centrifuged, and analyzed.  Results showed that the in vitro addition of copper in concentrations as low as .15 mM produced a significant decrease in the amount of polysome aggregates that could be recovered from endometrium.  Liver required concentrations of at least .3 mM to show a similar effect.  For concentrations of 1.5 mM copper, the recoverable amount of the polysome fraction from liver and endometrium was 40% and 25%, respectively, of that recoverable without added copper.  When the obtained polysomes were analyzed in a sucrose gradient, additions of increasing concentrations of copper induced a progressived decrease of the heavy components of the polysome patterns with a concomitant increase in the lighter  components.  The results which show copper as a possible dissociating agent of liver polysomes are important, but it is doubtful that liver concentrations of those required to dissociate polysomes (.3 mM) will ever be reached by the use of these IUDs.  It is postulated that the impairment in polysome aggregation is part of the mechanism of the copper IUD, and it may modify both the endometrial characteristics and the blastocyst viability required for successful embryo development.


Influence of ethanol on cardiovascular tolerance to +Gz acceleration.


Metabolic fate of leucine: a significant sterol precursor in adipose tissue and muscle.


Alternatives for vascular access for hemodialysis: experience with autogenous saphenous vein autografts and bovine heterografts.


Comparative study of seven media for sterility testing.


Oxytocin reproducing reflex milk ejection in lactating women.


Pentazocine radioimmunoassay.

Pentazocine radioimmunoassay.


Antiproteolytic activity of amyrin acetates.


PEG test: a new radioimmunoassay for the detection of hepatitis B antigen.


Phosphate-mediated alteration of the Microsporum gypseum germination protease specificity for substrate: enhanced keratinase activity. Inorganic phosphate was found to decrease the caseinolytic and ethyl-esterase activities of the Microsporum gypseum germination protease. The germination protease possessed exokeratinase (beta-keratinase) activity immediately after release from the fungal spore. After phosphate treatment of the enzyme, the germination protease also possessed endo-keratinase (alpha-keratinase) activity. Phosphate altered the protease's pH optimum from 9.0 to 7.0 and decreased the molecular weight from 33,000 to 16,000. These values were identical to those found for the keratinase. Alpha- and beta-keratinase activities were stimulated in excess of 200-fold by disulfide reducing agents. Natural and suspected keratin degradation products also enhanced keratinase activity. Cell fractionation and in vitro conversion of the alkaline germination protease into a functional keratinase suggested that the subunits comprising the germination protease and the keratinase were of a common origin.


Motivational control of retrograde amnesia: further explorations and effects.


Olfactory bulbectomy and mucosal damage: effects on copulation, irritability, and interspecific aggression in male rats.


Differences in arginine requirement for growth among arginine-utilizing Mycoplasma species. The essentiality of arginine for initiation of growth of arginine-utilizing, nonglycolytic Mycoplasma species from small populations was studied by growing the organisms in a semisynthetic medium proven to be free from arginine by chemical and biological assays. Initiation of growth of two strains of M. arginini did not require arginine, whereas another strain of M. arginini required 4 mM arginine, as did M. gallinarum. M. hominis grew in 0.4 mM arginine. A species which utilizes both arginine and glucose, N. fermentans, did not require arginine but did require glucose for growth. When mycoplasmata were grown in human heteroploid cell cultures employing medium free from arginine but supplemented with citrulline, similar results were obtained: two M. arginini strains grew in the absence of arginine, whereas growth of M. gallinarum and M. hominis and a third M. arginini strain was dependent on arginine even though mammalian cells were present. The arginine deiminases were heterogeneous serologically: antisera to M. hominis and M. arginini showed reciprocal inhibition of their enzymes but did not inhibit arginine deiminase from M. gallinarum. Antiserum to M. gallinarum inhibited only M. gallinarum enzyme.


Effects of olfactory bulbectomy and peripheral deafferentation on reactions to crowding in gerbils (Meriones unguiculatus).


Effects of genotype and postnatal experience on activity, avoidance, shock threshold, and open-field behavior of rats.


Immunopathology of aging.

Immunopathology of aging.


Involutional mammary duct ectasia and periductal mastitis in a male.


Effects of norepinephrine and dibenamine implanted in the median eminence on the estrous cycle of the rat. An attempt to clarify the physiological significance of monoamines in the mammalian median eminence (ME) by implanting them into the ME of Sprague-Dawley and Wister rats is presented. Norepinephrine (NE) and dibenamine (DB) were so implanted  and the effects on the estrous cycles and on the ovary were observed. NE and cholesterol (C) were mixed in ether in ratios of 3:1 or 1:1 by weight and dried. DB and C were mixed and dried in ratios or 4:1, 2:1, or 1:10. About 10 mg of each  mixture or C alone were implanted stereotaxically into the basal hypothalamus or into the adenohypophysis with the aid of an X-ray appearatus. Daily vaginal smears were made on all animals to determine estrous cycles. C alone did not change  the estrous cycles. NE plus C (1:1) when placed in the middle portion of the median-eminence-arcuate-nucleus region (MMAR) and posterior portion median-eminencearcuate region (PMAR) irregularly prolonged both the estrous and diestrous phases  and the ovaries had few follicles and atrophic corpora lutea. When the tip of the cannula was placed only in the arcuate nucleus (AN) area near the ME similar effects were noted. When NE-C (3:1) was implanted in the MMAR and PMAR regions,  rats showed estrous phases in 3-6 days. This result was followed by prolonged diestrous and then irregular estrous cycles. In rats implanted with DB-C in the MMAR and PMAR, a prolonged diestrous phase followed immediately. Traumatizing the  uterus of 5 of these animals produced a decidual reaction. A rat not so traumatized showed prolonged diestrus with intermittent 2-day estrus for about a month after implantation. After implantations with either C, NE-C, or DB-C, only NE-C  implanted in MMAR induced a marked decrease in uterine weight. When the implantations were made in the AN the ovaries did not show any marked chanes. The effects of NE seemed to result from a direct action on the ME itself. Thus NE, at the  level of the ME, suppressed the secretion of follicle stimulating hormone and luteinizing hormone. Results are thought to indicate that an alpha-adrenergic mechanism is involved in the release of a prolactin-inhibiting factor from the ME


Continuing education for the trauma surgeon.


Influence of storage on composition, amino acid content, and solubility of soybean leaf protein concentrate.


Solubility behavior of soybean globulins as a function of pH and ionic strength.


Mechanosensitive units in the upper bill and in the tongue of the domestic duck.


Influence of furosemide and d-l propranolol on glomerular filtration rate and renin secretion in the rat.


Thermosensitive units in the tongue and in the skin of the duck's bill.


The distribution of Rb86 in different parts of the liver.


Micromanipulator with an electrode driver used for microelectrode work.


A concentrically and radially adjustable holder for ten microelectrodes.


Analysis of chloride in milk with a specific ion electrode.


The availability for the rat of methionine and cystine contained in dry bean seed (Phaseolus vulgaris).


Effects of pregnancy and associated hormones in mouse intestine, in vivo and in vitro.


Mephenesin blocks early inward currents and strychnine-induced multiple discharges of aplysia neurons.


RBE and OER determinations for radium and californium 252.


Nonanal and 6-methyl-5-hepten-2-one: endogenous germination stimulators of uredospores of Puccinia graminis var. tritici and other rusts.


Infection by leptospira ballum: a laboratory-associated case.


Reassessment of a non-hemophilic reagent for factor VIII (AHF) determination.


Pulmonary amyloidoma.

Pulmonary amyloidoma.


Viral infection of megakaryocytes in varicella with purpura.


Two-dimensional immunoelectrophoresis of mycobacterial antigens. Comparison with a reference system.


Androgenic hormones and human granulopoiesis in vitro.


Hepatic erythropoietin production in the lead-poisoned rat.


Interaction of drugs with microtubule proteins.


Studies on cobalt myoglobins and hemoglobins. II. Kinetic studies of reversible oxygenation of cobalt myoglbins and hemoglobins by the temperature jump relaxation method.


Studies on cobalt myoglobins and hemoglobins. I. Preparation and optical properties of myoglobins and hemoglobins containing cobalt proto-, meso-, and deuteroporphyrins and thermodynamic characterization of their reversible oxygenation.


An expanded mechanism for rhodanese catalysis.


Nuclear magnetic resonance studies of lecithin-skatole interaction.


Studies on the mechanism of cobalamin binding to hog intrinsic factor.


The structure of a sulfated glycoprotein of chick allantoic fluid.


Molecular sieve studies of interacting protein systems. XI. Isomerizing solutes.


The effect of maternal age on decidualization in the mouse.


Studies on the structural and conformational basis for the relative resistance of serum and secretory immunoglobulin A to proteolysis.


Vitamin K1 content of infant formula products.


A method for demonstrating shortened platelet survival utilizing recovery from aspirin effect.


Letter: Screening for gonorrhea during pregnancy.


Spontaneous arteriovenous fistula arising from the external carotid artery during pregnancy. Case report.


Central serous retinopathy and pregnancy.


Treatment of glaucomatocyclitic crises.

Treatment of glaucomatocyclitic crises.


The Pickering report. V. A patient's bill of rights.


Plasma half-life of phenylbutazone in patients with impaired liver function.


Preparation and properties of anti-sulfatide serum.


Anti-inflammatory effects of tartar emetic and niridazole: suppression of schistosome egg granuloma.


Blocking activity induced by solubilized alloantigens.


Letter: Cellular proliferation can be an unreliable index of immune competence.


Effect of antiserum to human chorionic gonadotropin on growth and function of choriocarcinoma in vivo and in vitro.


Studies on a cell line derived from the L1210 murine leukemia with altered surface properties and decreased capacity for tumor production.


Protein deficiency and performance of pigeons on a multiple schedule of reinforcement.


Ultrasounds in small rodents: arousal-produced and arousal-producing.


Seizure susceptibility and avoidance conditioning in adult rats treated prenatally with chlorpromazine.


Blood supply to the bovine ovary.

Blood supply to the bovine ovary.


Effect of energy intake prior to parturition on milk lipids and survival rate, thermostability and carcass composition of piglets.


Fat utilization by rabbits.

Fat utilization by rabbits.


Effect of testosterone on reproductive characteristics of puberal gilts.


Uterine motility of the ewe during the estrous cycle.


Telemetry of uterine motility in the cycling ewe.


Estrus, ovulation rate and body composition of selected strains of mice on ad libitum and restricted feed intake.


Sequence of protein feeding and value of alfalfa meal and fish meal for pregnant gilts and sows.


Intrauterine administration of prostaglandin F2 alpha in cows: progesterone, estrogen, LH, estrus and ovulation.


Interaction of stage of the breeding season and intrauterine devices on estrous cycle length in the ewe. To determine whether the presence of IUDs would reduce estrous-cycle length throughout a breeding season, 51 and 58 ewes, respectively, were studied in 2 experiments.  The estrous cycles of ewes with an IUD adjacent to each ovary averaged 9.6 days in length from September through November, and nearly 20 days from December through February.  The same ewes showed a similar pattern in a second September-to-February breeding season.  In the third breeding season, ewes with an IUD in each uterine horn were laparotomized if they had not returned to heat within 9 days after a designated estrous period.  Of 54 ewes laparotomized during the course of the breeding season, corpora lutea 1) had regressed or were regressing in 49 (91%) by Day 9, and 2) had regressed in the other 5 ewes by Day 18.  It is concluded that cycles longer than 9 days were usually caused by 1 or more successive ovulations without estrus, and that the increased mean cycle length for IUD-bearing ewes during the second half of the breeding season was due  primarily to a greater frequency of ovulation without estrus.


Serum FSH in anestrous ewes treated with 17 beta-estradiol.


The repeal of PSRO.

The repeal of PSRO.


Proceedings: Utilization review, medical audit, and peer review.


Plasma and rumen fluid components of steers fed two sources and levels of nitrogen.


Fetus acardius.

Fetus acardius.


Placental-radiographic analysis of parasitic acardiac fetus: partially common umbilical circulation.


A community of human malformation syndromes involving the Müllerian ducts, distal extremities, urinary tract, and ears.


T.A.P. (trustee, administrator, physician) institutes.


PSRO.

PSRO.


Misuse of drugs act 1971.

Misuse of drugs act 1971. An attempt is made to explain the provisions of the Misuse of Drugs Act 1971 stressing, where possible, those portions of the Act which affect members of the medical profession.


Misuse of Drugs Regulations 1973.

Misuse of Drugs Regulations 1973.


Letter: The dog in our midst.

Letter: The dog in our midst.


Efficacy of participant modeling as a function of response induction aids.


Evidence for a structural requirement for the aggregation of platelets by collagen. This study investigates whether soluble collagen can initiate platelet aggregation or whether a higher degree of polymerization is required. Purified rat skin collagen was prepared in four states. Soluble monomeric collagen, containing 2 muM calcium chloride, was maintained at 4 degrees C until use. A previously uncharacterized form of collagen, soluble microfibrillar collagen, was prepared from monomeric collagen containing calcium chloride by allowing it to polymerize at 23 degrees C. Viscometric and electron microscopic characterization of microfibrillar collagen indicated polymerization to ordered native filaments. Particulate native macrofibrillar collagen was prepared from monomeric collagen by allowing it to polymerize at 37 degrees C in the absence of calcium. Particulate collagen, in which the fibers were randomly associated, was prepared by salt precipitation of calcium-free monomeric collagen. Microfibrillar and native macrofibrillar collagen initiated platelet aggregation, with a lag phase of approximately 60 s. Monomeric collagen initiated aggregation with a lag phase of approximately 180 s. The duration of the lag phase for platelet aggregation initiated by monomeric collagen was independent of the dose. Salt-precipitated particulate collagen did not initiate platelet aggregation. Agents which prolong the transition from monomeric collagen to fibrillar collagen (urea, arginine) retarded or prevented the aggregation of platelets by monomeric collagen. Sodium borohydride, which stabilizes the intraand intermolecular cross-links of collagen did not affect platelet aggregation. Penicillamine, which displaces the intermolecular cross-links and binds the intramolecular cross-links of collagen, did not prevent platelet aggregation. The data suggest that an architectural requirement exists for the initiation of self-perpetuating platelet aggregation; that tropocollagen units do not fulfill this requirement; that a soluble collagen preparation, microfibrillar collagen, contains the minimal structural unit; and that cross-linkages within collagen do not play a critical role in platelet aggregation.


Increased sensitivity of seminal vesicles to testosterone in a mouse strain with low plasma testosterone levels.


Does a mechanism of negative feedback determine the intermittent release of oxytocin during suckling?


Letter: Magnesium in Lesch-Nyhan syndrome.


A monoclonal IgM protein with antibody-like activity for human albumin. The serum of a patient (L'ec) with an IgM lambda monoclonal protein was noted to bind albumin on immunoelectrophoresis. Analytical ultracentrifugation of the L'ec serum demonstrated 23S and 12S peaks, but no 4S (albumin) boundary. Immunologically identical 20S and 9S IgM proteins were isolated from the serum and the addition in vitro of either the patient's albumin or albumin isolated from normal serum was shown to reconstitute the 23S and 12S boundaries. The binding of high molecular weight IgM to albumin was demonstated by Sephadex G200 chromatography with (125)I-labeled albumin and isolated IgM. Immunoelectrophoresis of the L'ec IgM developed with aggregated albumin (reverse immunoelectrophoresis) also demonstrated the binding of albumin to IgM. That all of the patient's IgM complexed with albumin was shown by affinity chromatography employing an aggregated albumin-immunoadsorbent column. Binding was shown to be of the noncovalent type by polyacrylamide gel electrophoresis in 8 M urea. With hot trypsin proteolysis, Fabmu and Fcmu5 fragments were isolated, and monomer albumin was shown to complex only with the Fabmu fragment by both analytical ultracentrifugation and molecular sieve chromatogaphy employing (125)I-labeled Fab fragments. 1 mol of Fabmu fragment bound 1 mol of monomer albumin. Polymers of human albumin, produced by heat aggregation, precipitated with the isolated L'ec protein on gel diffusion analysis and, when coated on sheep red blood cells, gave a hemagglutination titer greater than 1 million with the whole L'ec serum. 50 additional monoclonal IgM, 33 IgA, and 80 IgG sera failed to show precipitation or hemagglutination with aggregated albumin. Native monomer albumin inhibited precipitation only at high concentrations (> 50 mg/ml); dimer albumin or fragments of albumin produced by trypsin digestion inhibited at low concentrations (0.4 mg/ml). No reactivity occurred with the albumin of five other mammalian species, including bovine. The L'ec protein has the characteristics of an antibody against aggregated albumin, which also has reactivity with native (monomer) albumin. This system shares many similarities with the reaction of IgM human rheumatoid factors with IgG antigen.


Letter: Contraceptive choice and cervical cytology. In the evaluation of biological potentials of contraceptives in the genesis of cervical cancer, cytology seems to have been given due attention while information on the clinical condition and specific treatment of the target organ, the  cervix, is repeatedly neglected.  "Cancer does not develop in a healthy cervix" is an old dogma derived from impressions gained by clinical examination of the organ.  This concept is supported by epidemiologic studies indicating that cancer is extremely rare in cervices restored to a normal condition by cauterization.  It may be postulated that this type of treatment destroys the permissive target cells of the cervix, thus indirectly rendering possible coitus-associated and contraceptive-associated(?) mutagen(s) invalid.  Also, in comparative studies, an evaluation should be made of the significance of a higher incidence of cervical ectopy reported in women receiving oral hormonal contraceptives.  The importance of ectopy in the genesis of cervical malignancy has been derived from the presumption that permissive cervical cells are thus created and exposed to vaginal contents which may harbor the mutagens(s).  Therefore, it would seem not only desirable but imperative to include the neglected parameters of clinical condition and of specific treatment (both past and present) of the uterine cervix in the structure of studies like the recent one in  this Journal on "Contraceptive choice and cervical cytology", by Drs. Shulman and Merritt.  Still another parameter which might prove to be of significance is the predominant type of vaginal flora, which can be assessed readily in the routine examination of Papanicolaou smears.  It may be that vaginal contents, which would seem to be somewhat different in wearers of the intrauterine contraceptive device as compared to "pill" users, might play a part in the advent of cervical malignancy as a medium of or for  coitus-associated mutagens(s).


Effects of a copper intrauterine contraceptive device on embryo survival, uterine spermicidal activity, and endometrial vascular porosity in the ewe.


3 alpha-androstanediol in human peripheral plasma.


Congenital syndrome in Hereford calves with kyphoscoliosis, arthrogryposis and palatoschisis.


Catalepsy induced by alpha-methyl-p-tyrosine and d-amphetamine: the rôle of catecholamine metabolism.


Proceedings: Distribution of retroperitoneal lymph node metastases in testicular germinal tumors.


Position paper on recommended salaries and employment practices for members of The American Dietetic Association.


Echocardiographic observations in idiopathic hypertrophic subaortic stenosis.


Letter: Tetracycline in pediatric practice.


Temporal variation of adenine ribonucleotides during the cell cycle of Chinese hamster fibroblasts in culture.


Characterization of mesozoan DNA.

Characterization of mesozoan DNA.


DNA-binding proteins of Xenopus laevis: synthesis during oogenesis-ovulation and early embryogenesis.


Differential chromosomal sensitivities of aneusomic cell clones to UV light.


Estrus, ovulation, progesterone and luteinizing hormone after prostaglandin F2a in mares.


Serum progesterone levels in hysterectomized pseudopregnant rabbits.


Changes in plasma zinc related to fasting and dietary protein intake of Japanese quail.


Intermale aggression in mice: modification by postneonatal castration.


Prenatal interference with the onset of puberty, vaginal cyclicity and subsequent pregnancy in the female rat.


Effect of prostaglandin F2A (PGF2a) on venous contractility and 45Ca uptake.


The effect of inosine on blood pressure in the rat.


Radioimmunoassay for dexamethasone in plasma.


The effects of methyl-5(6)-butyl-2-benzimidazole carbamate (parbendazole) on reproduction in sheep and other animals. Introduction.


Emergency cesarean hysterectomy.

Emergency cesarean hysterectomy.


Lipid oxidation and fatty acid changes in beef combined with vegetables and textured vegetable protein.


Cookie for a low-phenylalanine diet.

Cookie for a low-phenylalanine diet.


Perinatal tips.

Perinatal tips.


Analysis of cortical urea sequestration in rat renal tissue slices.


Amplitude dependence of the carotid sinus reflex.


Effect of elevated lactataemia on the rates of lactate turnover and oxidation in rats.


A study of the rheological properties of a non-Newtonian fermentation broth.


The anterior glands of adult Necator americanus (Nematoda: Strongyloidea). II. Cytochemical and functional studies.


The effects of parathyroid hormone, colchicine, and calcitonin on the ultrastructure and the activity of osteoclasts in organ culture. The ultrastructure of osteoclasts was examined in fetal rat bones after stimulation or inhibition of resorption in culture. A central ruffled border area completely encircled by a clear zone was considered to represent the resorbing system of the cell. The proportion of ruffled border and clear zone in osteoclast cross sections was compared with changes in bone resorption as measured by the release of previously incorporated radioactive calcium ((45)Ca). In control cultures 55% of the osteoclast cross sections showed an area closely apposed to bone and this consisted mainly of clear zone; only 11% showed ruffled borders. Treatment with parathyroid hormone (PTH) increased (45)Ca release, increased the frequency of finding areas closely apposed to bone (79%), and markedly increased the frequency of the ruffled border area (64%). Colchicine given concurrently with PTH decreased the number of osteoclasts. Colchicine or calcitonin treatment after PTH stimulation decreased the proportion of ruffled border area significantly by 1 h; this was followed by a decrease in (45)Ca release. These inhibited osteoclasts resembled osteoclasts from control, unstimulated cultures, suggesting that the cells had returned to their inactive state. Colchicine-treated osteoclasts also showed a loss of microtubules and a massive accumulation of 100 A filaments, suggesting that synthesis of microtubular subunits had increased.


Cell cycle-specific changes in histone phosphorylation associated with cell proliferation and chromosome condensation. Preparative polyacrylamide gel electrophoresis was used to examine histone phosphorylation in synchronized Chinese hamster cells (line CHO). Results showed that histone f1 phosphorylation, absent in G(1)-arrested and early G(1)-traversing cells, commences 2 h before entry of traversing cells into the S phase. It is concluded that f1 phosphorylation is one of the earliest biochemical events associated with conversion of nonproliferating cells to proliferating cells occurring on old f1 before synthesis of new f1 during the S phase. Results also showed that f3 and a subfraction of f1 were rapidly phosphorylated only during the time when cells were crossing the G(2)/M boundary and traversing prophase. Since these phosphorylation events do not occur in G(1), S, or G(2) and are reduced greatly in metaphase, it is concluded that these two specific phosphorylation events are involved with condensation of interphase chromatin into mitotic chromosomes. This conclusion is supported by loss of prelabeled (32)PO(4) from those specific histone fractions during transition of metaphase cells into interphase G(1) cells. A model of the relationship of histone phosphorylation to the cell cycle is presented which suggests involvement of f1 phosphorylation in chromatin structural changes associated with a continuous interphase "chromosome cycle" which culminates at mitosis with an f3 and f1 phosphorylation-mediated chromosome condensation.


Cyclic changes in the cell surface. II. The effect of cytochalasin B on the surface morphology of synchronized Chinese hamster ovary cells. The surface morphology of Chinese hamster ovary cells treated with cytochalasin B (CB) has been examined using the scanning electron microscope. The cells respond to treatment with CB by retracting peripheral processes, rounding up, and assuming a smooth or gently convoluted surface. This response occurs within minutes. Cells in different stages of the cell cycle all respond in a similar manner. When CB is removed from treated cells by washing with conditioned medium, the cells regain their normal surface conformation within minutes. The surface topography of these released cells is characteristic of their stage in the cell cycle. Because CB causes an alteration in the morphology of the cell surface and because of the speed of the response and recovery, it is proposed that the primary site of action of CB is the cell surface.


Cyclic changes in the cell surface. I. Change in thymidine transport and its inhibition by cytochalasin B in Chinese hamster ovary cells. Cytochalasin B (CB) shows a marked concentration-dependent inhibition of the incorporation of [(3)H]thymidine into Chinese hamster ovary cells. This inhibition was shown to result from an inhibition of thymidine uptake, not from an inhibition of DNA synthesis. Cells normally acquire the capacity to transport thymidine as they move from the G1 stage of the cell cycle into the S phase. If CB is added to cells while they are in G1, they do not acquire the ability to transport thymidine as they enter S. However, the addition of CB to cells that are already in S has no effect on their ability to transport thymidine. These results are discussed in terms of a model in which elements involved in thymidine transport enter the cell surface membrane as the cells move from G1 to S. It is proposed that CB prevents this structural transition by binding to the cell surface.


Cell death in the embryonic chick spinal cord. The events which occur in the death of visceromotor neurons of the cervical region of the chick embryo's spinal cord have been analyzed by electron microscopy. These normal degenerative events are compared with those in the lumbosacral cord where nerve cell death was induced by removal of peripheral organs. The initial set of degenerative changes include a decrease in nuclear size, the clumping of chromatin beneath the nuclear envelope, an increase in electron opacity of the cells, the disappearance of Golgi bodies, and the disaggregation of polysomes. These events are followed by the loss of the nuclear envelope and most of the endoplasmic reticulum, the appearance of bundles of filaments, and the formation of many ribosome crystals. Ribosome crystals are seen only in the dying cells. Their abundance may indicate a drastic reduction in RNA synthesis as one of the initial events which lead to the death of these neurons. The neurons are finally subdivided and engulfed by cells of the normal glial population, and further breakdown of the cell fragments occurs in large phagocytic vesicles of the gliocytes.


Stimulation of hepatic beta-hydroxy-beta-methylglutaryl coenzyme A reductase activity in allylamine-fed rats.


Mathematical analysis of DNA distributions derived from flow microfluorometry.


Reduced permeability in CHO cells as a mechanism of resistance to colchicine.


Density dependent regulation of growth in L cell suspension cultures. 3. Elevation of specific activity of acetylcholinesterase.


Chinese hamster cells exhibiting a temperature dependent alteration in purine transport.


Induction of lysosomal enzymes in contact inhibited 3T3 cells.


Editorial: PSRO is here.

Editorial: PSRO is here.


Selection for temperature-sensitive mutants of diploid and tetraploid mammalian cells.


Selective 3-(O-carboxymethyl)oxime formation in steroidal 3,20-diones for hapten immunospecificity.


Physiologic response to intense summer heat and its effect on the estrous cycle of nonlactating and lactating Holstein-Friesian cows in Arizona.


Trisomy in a feline fetus.

Trisomy in a feline fetus.


Drug metabolism in cell culture. I. Importance of steric factors for activity in porphyrin-inducing drugs.


Effect of acute ethanol administration on rat plasma protein synthesis.


Uricase. Subunit composition and resistance to denaturants.


Asymmetrical hemoglobin hybrids. An approach to the study of subunit interactions.


Faster and easier radioimmunoassay of digoxin.


Chromogenic-substrate method for demonstrating multiple forms of alpha-amylase after electrophoresis.