Treatment of the seriously disturbed preschool child.


Infantile autism: status of research.

Infantile autism: status of research.


The preschool child: dilemmas of diagnostic labeling.


Retraining of aggressive boys by their parents: review of recent literature and follow-up evaluation.


The disturbed child: the back of the hand to the past, and the shrug to the future.


The measurement of retrograde post-traumatic amnesia.


The processes of psychiatric residency training. I. Selection of trainees and the outcome of training.


The processes of psychiatric residency training. II. Trainees who drop out.


Letter: Mental retardation services.

Letter: Mental retardation services.


Detection of heterozygotes for myotonic dystrophy.


The antenatal diagnosis of trisomy 18.

The antenatal diagnosis of trisomy 18.


Chromosomes and human infertility. I. Mitotic and meiotic chromosome studies in 202 consecutive male patients.


Congenital metatarsus varus in four generations.


Hereditary tyrosinemia in the province of Quebec: prevalence at birth and geographic distribution.


Familial aggregation in bilateral renal agenesis.


Probably monozygotic twins with discordance for Goldenhar syndrome.


Application of a quantitative scoring system in the investigation of some hereditary neurological disorders.


Anthropometry in sex chromosome abnormality.


Editorial: Data bank use in management of chronic disease.


Data processing by computer: on-line or off-line?


An information retrieval procedure for a cancer-treatment data base system.


A method for finding the maximun of a function fo several variables suitable for use on a programmable desk calculator.


Central regulation of temperature in cold environments. II. A dynamic model with three temperature inputs.


Stimulatory effects of thyroxine administration on reducing-equivalent transfer from substrate to oxygen during hepatic metabolism of sorbitol and glycerol.


Inhibition of rat-liver RNA polymerase in vitro by aflatoxin B1 in the presence of microsomal fraction.


Transient kinetics of benzaldehyde formation during the catalytic action of pig-plasma benzylamine oxidase.


Isolation and study of the composition of a peptidoglycan complex excreted by the biotin-requiring mutant of Brevibacterium divaricatum NRRL-2311 in the presence of penicillin.


Separation and characterisation of subunits of histidine decarboxylase from Micrococcus sp.n.


The denaturation of lactoferrin and transferrin by urea.


Purification and some physical properties of a chymotrypsin-like protease of the larva of the hornet, Vespa orientalis.


The reaction of the iron-sulfur protein hydrogenase with carbon monoxide.


Determination of binding parameters from Scatchard plots. Theoretical and practical considerations.


A kinetic study of the isozymes determined by the three human phosphoglucomutase loci PGM1, PGM2, and PGM3.


Structure of the porcine vasoactive intestinal octacosapeptide. The amino-acid sequence. Use of kallikrein in its determination.


Spectral properties of rat adrenal-mitochondrial cytochrome P-450.


Regulation of isoproterenol-stimulated DNA synthesis in rat salivary gland in vivo by adrenal glucocorticoids.


Age-dependent regulation of isoproterenol-stimulated DNA synthesis in rat salivary gland in vivo.


Age-dependent regulation of mammalian DNA synthesis and cell division in vivo by glucocorticoids.


Tritiated corticosteroid metabolism in intact and adenohypophysectomized ducks (Anas platyrhynchos).


Plasma testosterone levels during the testicular cycle of the redwinged blackbird (Agelaius phoeniceus).


The effects of eyestalk ablation and B-ecdysone on RNA synthesis in the androgenic glands of the protandric shrimp, Pandalus platyceros Brandt.


The effects of melatonin treatment on gonosomatic index in the teleost, Fundulus similis, and the tree frog, Hyla cinerea.


Endocrine control of clutch size in reptiles. 3. Delta5-3beta-hydroxysteroid dehydrogenase activity in different-sized ovarian follicles of Anolis carolinensis.


Luteinizing hormone (LH) in the reptilian pituitary gland.


Plasma testosterone titers during the annual reproductive cycle of starlings (Sturnus vulgaris).


The pharmacological characterization of arginine vasotocin in the pituitary of the pacific hagfish (Polistotrema stoutii).


Control of haemolymph protein synthesis and oocyte maturation by the corpora allata in female adults of locusta migratoria (Orthoptera): role of the blood-forming tissue.


Melatonin in the chicken brain: its origin, diurnal variation, and regional distribution.


The organ of Zuckerkandl of the golden hamster (Mesocricetus auratus).


Failure of sex hormones and PMS to stimulate gonadal development in young Rana nigromaculata.


Induction of oviposition in the domestic hen by prostaglandins.


Drug-related deaths in the United States--first decade of an epidemic.


The Mount Sinai-HIP joint program.

The Mount Sinai-HIP joint program.


Health services in a Soviet auto plant.

Health services in a Soviet auto plant.


Survey of need for a new professional: the clinic liaison educator.


Distribution of visual acuity in Egypt.

Distribution of visual acuity in Egypt.


The impact of legal abortion: redefining the maternal mortality rate.


The challenge of neonatal mortality in an urban hospital.


Three case studies in planning: II. Financial planning for an HMO.


Three case studies in planning: I. Predicting hospital bed needs.


Financing maternity care.

Financing maternity care.


Cost effectiveness of early detection of disease.


Emergency medical services: the research gaps.


Operating surgeon held responsible for sponge error.


Coping with regulation.

Coping with regulation.


Three case studies in planning: 3. Use of decision theory in regional planning.


Planning a medical practice using paramedical personnel.


Alternative conceptions of accountability. I.


Service costs and management: the man-hours variable.


Interview with Sr. Grace Marie Hiltz, president of the Catholic Hospital Association.


Understanding the implementing of change.


HMOs: organizational and financial models.


A geographic analysis of counties without an active non-Federal physician, United States, 1963-71.


Pesticides and human health--an epidemiologic approach.


Better management of people resources.

Better management of people resources.


The tutor system: an alternative to the pathology chief residency.


Organization of departments of laboratory medicine and clinical pathology in medical schools.


The incidence and histogenesis of vaginal adenosis. An autopsy study.


Gynecology.

Gynecology.


Methoxyflurane nephropathy.

Methoxyflurane nephropathy.


Prognostic factors in cutaneous malignant melanoma. A comparative study of long term and short term survivors.


Hypertrophic infundibular stenosis of the calyces of the kidney.


Planning for the economic and career impact of a sickle cell anemia program.


Esthesioneuroblastoma: a light and electron microscopic study.


Editorial: Malakoplakia: an idea whose time has come.


Editorial: Pathologists and the Food and Drug Administration.


Letter: The American "balloon" of pathology.


Influence of phenformin on gastric emptying rate.


Effects of internal divalent cations on voltage-clamped squid axons. We have studied the effects of internally applied divalent cations on the ionic currents of voltage-clamped squid giant axons. Internal concentrations of calcium up to 10 mM have little, if any, effect on the time-course, voltage dependence, or magnitude of the ionic currents. This is inconsistent with the notion that an increase in the internal calcium concentration produced by an inward calcium movement with the action potential triggers sodium inactivation or potassium activation. Low internal zinc concentrations ( approximately 1 mM) selectively and reversibly slow the kinetics of the potassium current and reduce peak sodium current by about 40% with little effect on the voltage dependence of the ionic currents. Higher concentrations ( approximately 10 mM) produce a considerable (ca. 90%) nonspecific reversible reduction of the ionic currents. Large hyperpolarizing conditioning pulses reduce the zinc effect. Internal zinc also reversibly depolarizes the axon by 20-30 mV. The effects of internal cobalt, cadmium, and nickel are qualitatively similar to those of zinc: only calcium among the cations tested is without effect.


Solute flux coupling in a homopore membrane. Our previous studies on solute drag on frog skin and synthetic heteropore membranes have been extended to a synthetic homopore membrane. The 150-A radius pores of this membrane are formed by irradiation and etching of polycarbonate films. The membrane is 6-microm thick and it has 6 x 10(8) pores cm(-2). In this study, sucrose has been used as the driver solute with bulk flow blocked by hydrostatic pressure. As before on heteroporous membranes, the transmembrane asymmetry of tracer solute is dependent on the concentration of the driver solute. Tracer sucrose shows no solute drag while maltotriose shows appreciable solute drag at 1.5 M sucrose. With tracer inulin and dextran, solute drag is detectable at 0.5 M sucrose. These results are in keeping with the previous findings on heteropore membranes. Transmembrane solute drag is the result of kinetic and frictional interaction of the driver and tracer solutes as the driver flows down its concentration gradient. The magnitude of the tracer flux asymmetry is also dependent on the size of the transmembrane pores.


Xylitol--clinical pharmacology in normal adult volunteers.


Kinetics of the opening and closing of individual excitability-inducing material channels in a lipid bilayer. The kinetics of the opening and closing of individual ion-conducting channels in lipid bilayers doped with small amounts of excitability-inducing material (EIM) are determined from discrete fluctuations in ionic current. The kinetics for the approach to steady-state conductance during voltage clamp are determined for lipid bilayers containing many EIM channels. The two sets of measurements are found to be consistent, verifying that the voltage-dependent conductance of the many-channel EIM system arises from the opening and closing of individual EIM channels. The opening and closing of the channels are Poisson processes. Transition rates for these processes vary exponentially with applied potential, implying that the energy difference between the open and closed states of an EIM channel is linearly proportional to the transmembrane electric field. A model incorporating the above properties of the EIM channels predicts the observed voltage dependence of ionic conductance and conductance relaxation time, which are also characteristic of natural electrically excitable membranes.


Ion diffusion selectivity in lecithin-water lamellar phases. The diffusion coefficients of Na(+), Rb(+), and cl(-) were determined in lecithin-water lamellar phases at 18 degrees C as a function of phase hydration. Diffusion was measured within the phase with no transfer between phase and bulk aqueous medium. The relative diffusion coefficients of anion and cation depended strongly on phase hydration. At low water content, the diffusion coefficient of Cl(-) was greater than that of Na(+) or Rb(+) whereas at high water content both cations diffused faster than the anion. The change in relative diffusion coefficient occurred at 0.24 g water/g phase (24% water). The possibility that a change in conformation of the lecithin polar head occurs at a phase water content of 24% is considered. The diffusion coefficients of all three ions decreased at the water content where the relative diffusion rates inverted. Freeze fracture and polarizing microscopy studies were carried out to obtain information on phase structure. The latter study indicated that a change in long-range organization of the phase occured at 24% water. This change accounts for the decrease in the ion diffusion coefficients at this water content. The change in conformation of the choline phosphate group proposed as an explanation for the change in ion selectivity could lead to changes in long-range organization of the phase as a second order-effect.


Fixed combination prescription drugs: FDA policy.


Disseminated intravascular coagulation as a consequence of cerebral damage. Three cases with intracranial lesions developed evidence of disseminated intravascular coagulation which was confirmed at necropsy. The factors engendering this state, including release of potent thromboplastin from neural tissue are discussed and the danger of this intermediary mechanism of disease increasing the mortality of intracranial disease is demonstrated. Careful haematological investigation of all patients with intracranial disease is therefore advised, especially if they manifest evidence of a bleeding tendency.


Fast electrical potential from a long-lived, long-wavelength photoproduct of fly visual pigment. A rapid electrical potential, which we have named the M-potential, can be obtained from the Drosophila eye using a high energy flash stimulus. The potential can be elicited from the normal fly, but it is especially prominent in the mutant norp A(P12) (a phototransduction mutant), particularly if the eye color pigments are genetically removed from the eye. Several lines of evidence suggest that the M-potential arises from photoexcitation of long-lived metarhodopsin. Photoexcitation of rhodopsin does not produce a comparable potential. The spectral sensitivity of the M-potential peaks at about 575 nm. The M-potential pigment (metarhodopsin) can be shown to photoconvert back and forth with a "silent pigment(s)" absorbing maximally at about 485 nm. The silent pigment presumably is rhodopsin. These results support the recent spectrophotometric findings that dipteran metarhodopsin absorbs at much longer wavelengths than rhodopsin. The M-potential probably is related to the photoproduct component of the early receptor potential (ERP). Two major differences between the M-potential and the classical ERP are: (a) Drosophila rhodopsin does not produce a rapid photoresponse, and (b) an anesthetized or freshly sacrificed animal does not yield the M-potential. As in the case of the ERP, the M-potential appears to be a response associated with a particular state of the fly visual pigment. Therefore, it should be useful in in vivo investigations of the fly visual pigment, about which little is known.


Fibrin-fibrinogen degradation products in cerebrospinal fluid of patients admitted to a psychiatric unit. Paired cerebrospinal fluid (CSF) and serum samples collected from 81 of 241 patients admitted to a district psychiatric hospital during a six month period were assayed for fibrin/fibrinogen degradation products (FDP) using a haemagglutination inhibition technique. FDP were found in all serum samples. Fifteen patients (18·5%) had FDP in the CSF (range 0·7-3·75 μg/ml.) and of these 13 (87%) had associated CSF protein abnormalities and 9 (60%) were hypertensive. Mean serum FDP values were the same (4·4 μg/ml.) in patients with and without FDP in the CSF. Three patients had raised serum FDP concentrations but no FDP in the CSF. The evidence suggests that the presence of FDP in CSF indicates recent central nervous system damage. In this series the most common cause was vascular disease.


Cerebral damage in neonates resulting from arteriovenous malformation of the vein of Galen. Seven neonates with arteriovenous malformation of the vein of Galen are described. Six presented with cyanotic heart failure. Two cases had antenatal cerebral infarction, five periventricular leukomalacia, and three recent haemorrhagic infarction. Cerebral damage due to the malformation suggests that surgical correction of the arteriovenous malformation offers little to neonates, though after the neonatal period individuals can be cured by operation.


Extrapyramidal dysfunction with cerebral arteriovenous malformations. Arteriovenous malformations have only rarely been implicated as a cause of basal ganglia dysfunction. In four instances where such a lesion was uncovered, abnormal involuntary movements were present. In two, tremor involving the contralateral limbs occurred, while in others the head and neck were involved in dystonic movements and posture. The clinical and angiographic characteristics of these four patients have been assessed and are presented in detail in this report. The possible mechanism by which arteriovenous malformations may disturb the internal circuitry of the basal ganglia and induce symptoms are discussed.


Pneumocephalus: an unusual cause.

Pneumocephalus: an unusual cause. A 60 year old male had an open thoracotomy for bronchogenic carcinoma. On the twelfth hospital day he became obtunded and complained of headache. Radiographs revealed intracranial air. It was thought that the pneumocephalus in this patient was most likely secondary to a tension pneumothorax continuously forcing air through a dural tear sustained at the time of initial surgery. The causes of pneumocephalus are reviewed and no similar case report has been found in the literature.


Traumatic subarachnoid-pleural fistula.

Traumatic subarachnoid-pleural fistula. The case of a child suffering from traumatic bilateral subarachnoid-pleural fistula is reported and the possible mechanisms responsible are discussed.


Bioavailability studies of acetaminophen and nitrofurantoin.


Letter: Drug elimination and renal function.


Post-hyperventilation apnoea in patients with brain damage. A study of 100 subjects has confirmed that brief voluntary hyperventilation commonly causes apnoea in patients with supramedullary lesions, but not in healthy people. Apnoea was related to drowsiness rather than to the extent of the lesion; it was unrelated to the measured reduction in end-tidal carbon dioxide tension.


Effect of the anabolic protein hormone prolactin on human erythropoiesis.


Intercostal nerve conduction and posterior rhizotomy in the diagnosis and treatment of thoracic radiculopathy. Intercostal nerve conduction study has proved to be an accurate technique in diagnosis of thoracic radiculopathy in 161 patients, 80 of whom had subsequent posterior rhizotomy with relief of pain in 81% of those undergoing surgery. The only significant complication of intercostal nerve conduction study is an 8·8% incidence of pneumothorax.


Restoration of energy-linked functions in "aging" rat-liver mitochondria.


l-Acetylmethadol (LAM) treatment of opiate dependence: plasma and urine levels of two pharmacologically active metabolites.


Electrophysiological findings in entrapment of the median nerve at wrist and elbow. In 117 consecutive patients with carpal tunnel syndrome and 11 patients with a compression syndrome of the median nerve at elbow, motor and sensory conduction along the median and ulnar nerves and quantitative electromyography were compared with findings in 190 normal controls of the same age. In 25% of patients with carpal tunnel syndrome in whom motor conduction and EMG were normal, the lesion was located from abnormalities in sensory conduction. The fact that conduction along the same fibres was moderately slowed from digit to palm, severely slowed across the flexor retinaculum, and normal from wrist to elbow indicates that slowing was due to demyelination at the site of compression. Fifteen per cent of the patients with carpal tunnel syndrome had clinical and electrophysiological signs of ulnar involvement. In the other patients conduction along the ulnar nerve was as in 100 normal controls. Compression at the elbow was located by electromyographical findings rather than by abnormalities in conduction.


Effects of methamphetamine on daily rhythms of hypothalamic norepinephrine, serotonin and plasma corticosterone levels in the rat.


Glucose and octanoate utilization by isolated adult rat heart cells.


LH inhibitory properties of aqueous extracts of rat pineal glands.


Effect of hypoxic hypoxia and carbon monoxide on food intake, water intake, and body weight in two strains of rats.


Increase of hexobarbital sleeping time and inhibition of drug metabolism by the major metabolite of diphenylhydantoin.


The effect of folate and folate analogs upon dihydrofolate reductase and DNA synthesis in kidneys of normal mice.


Two sex pheromone components of the tobacco budworm moth, Heliothis virescens.


Dietary induction of hepatic malic enzyme activity: differentiation of the induction process.


Editorial: Continuing medical education: what lies ahead?


Editorial: Electro-medical safety.

Editorial: Electro-medical safety.


Results of screening for phaeochromocytoma in 17 hypertensive patients with episodic symptoms.


Hiatus hernia repair. Clinical and radiological results of a new combined thoracoabdominal technique.


Mortality from cardiovascular disease.

Mortality from cardiovascular disease.


Cultural interaction in aboriginal education. Dreamtime in Bourke?


Cultural interaction in aboriginal education. Dreamtime in Bourke? Comment 1. Cultural identity: race, intelligence and education: a reply.


Financial responsibility for continuing medical education.


Cultural interaction in aboriginal education. Dreamtime in Bourke? Comment 2. Experience on the Darling.


Letter: Sputum cultures.

Letter: Sputum cultures.


Letter: Renal tract malformations.

Letter: Renal tract malformations.


Letter: Splenectomy in haemophilia.

Letter: Splenectomy in haemophilia.


Letter: Lead poisoning.

Letter: Lead poisoning.


Letter: Subcutaneous lumbar rhizolysis.

Letter: Subcutaneous lumbar rhizolysis.


Serum ferritin and iron enrichment.

Serum ferritin and iron enrichment.


Letter: Utility of preparations of nitrofurantoin.


Letter: Lack of teratogenic effects in a presumed parvovirus epidemic.


Letter: Calcium oxalate urinary-tract stones in patients on maintenance dialysis.


Letter: Monitoring cancer with plasma carcinoembryonic antigen.


Letter: Why the yawn?

Letter: Why the yawn?


Letter: The "no-care" syndrome.

Letter: The "no-care" syndrome.


Some effects of chlordiazepoxide and chlorpromazine on response force in extinction.


Effect of pharmacologically-induced arousal on the evoked potential in the unanesthetized rat.


Morphine analgesia, two-way avoidance, and consummatory behavior following lesions in the midbrain raphe nuclei of the rat.


Short-term influence of intra-ventromedial hypothalamic administration of insulin on feeding in normal and diabetic rats.


Rhytidectomy in the male.

Rhytidectomy in the male.


Residency participation in out-of-country medical service.


Letter: Correcting prominent ears.

Letter: Correcting prominent ears.


Tripod resection for "pinocchio" nose deformity.


Editorial: Alcoholism as a disease.

Editorial: Alcoholism as a disease.


Anticonvulsant activity of delta-8- and delta-9-tetrahydrocannabinol in rats.


Tryptophan metabolism in depressive illness.


The EEG in antisocial behaviour: a study of posterior temporal slow activity in special hospital patients.


Diagnostic criteria of English, French, and German psychiatrists.


The speed of recall of pleasant and unpleasant experiences.


Plasma prolactin and luteinizing hormone levels in anorexia nervosa.


Possible stimulatory role of brain dopamine in the copulatory behavior of male rats.


Behavioral tolerance to marihuana as a function of amount of prior training.


Editorial: Fibre-optic endoscopy.

Editorial: Fibre-optic endoscopy.


Letter: Clonidine in hypertension.

Letter: Clonidine in hypertension.


On the role of serotonin in apomorphine-induced locomotor stimulation in rats.


Puromycin as an inhibitor of rat brain acetylcholinesterase.


Intelligence measurement and mental disorder.


Upper gastro-intestinal fibre-optic endoscopy. A 3-year study at Groote Schuur Hospital.


Serous effusions. An attempt at a clinical-pathological correlation.


The African's concept of the causes and treatment of epilepsy and convulsions.


Salivary flow as measures of parasympathetic nervous system involvement in children's asthma.


Methadone pseudowithdrawal syndrome: paradigm for a psychopharmacological model of opiate addiction.


Corticosterone, prolactin, and growth hormone responses to handling and new environment in the rat.


Editorial: Potassium.

Editorial: Potassium.


Personality factors and desynchronization of circadian rhythms.


The three investigators study. Serum uric acid, cholesterol, and cortisol variability during stresses of everyday life.


Proceedings: Physiology of potassium metabolism.


Proceedings: Studies of body potassium--interpretation.


Psychological correlates of childbirth pain.


Cytotoxic effect of alcohol on liver cells and fibroblasts in vitro.


Editorial: Hypertension and the community.


Proceedings: Potassium and the alimentary tract.


Acetylcholine and depression.

Acetylcholine and depression.


Proceedings: A multi-centre study of potassium deficiency in the elderly.


Proceedings: Potassium in surgical practice.


Proceedings: Potassium and renal disease.


Proceedings: Potassium and cardiac disease.


Proceedings: Haematinics and potassium metabolism.


Aggressiveness and testosterone measures in man.


Proceedings: Diuretics and potassium metabolism.


Evaluation of radioactive labelled phosphates in diagnosis of skeletal metastases.


Biochemical and electron-microscopical studies of chronic lead exposure in the heart and other organs in rats.


Editorial: Listen and look.

Editorial: Listen and look.


Abnormal drug metabolism following barbiturate and paracetamol overdosage.


"Gnaw warts".

"Gnaw warts".


Olfactory neuroblastoma.

Olfactory neuroblastoma.


Macrodactyly of the foot: surgical syndactyly after wedge resection.


Endometriosis of the colon: report of 14 patients requiring partial colectomy.


Normal shoulder variations in the technetium 99m polyphosphate bone scan.


Fungal vs drug-induced onycholysis.

Fungal vs drug-induced onycholysis.


The hysterical personality: identifying and managing one of the problem patients of medical practice.


Medical and surgical experiences in patients of a large southern stroke center.


Improved diagnosis of hyperparathyroidism: use of serum-ionized calcium and tubular reabsorption of calcium.


Factitious blood disease.

Factitious blood disease.


Adverse reactions to heroin use.

Adverse reactions to heroin use.


Removal of an embolized Pudenz catheter from the right ventricle using a snare-loop catheter.


Infectious mononucleosis meningoencephalitis.


Ocular metastasis of bladder cancer.

Ocular metastasis of bladder cancer.


Isolation and characterization of a prealbumin activator of prekallikrein from acetone-activated human plasma.


A search for fibrinopeptides in urine during experimental intravascular coagulation in dogs.


Further variant of the thrombocytopathy with abnormalities in platelet release reaction.


Major operations, hemostatic parameters and venous thrombosis.


Studies on soluble fibrin in plasma. V. Isolation and characterization of the clottable proteins obtained from patient plasmas upon gelation with ethanol.


The effect propranolol, alprenolol and practolol on the vibrinolytic and factor VIII responses to adrenaline and salbutamol in man.


Growth rate and volume of haemostatic plugs in the mesentery of normal and thrombocytopenic rabbits.


Editorial: Bleeding time, other in vivo hemostasis tests and the arrest of hemorrhage.


Letter: Leucocyte migration. The effect of heparin and preservative.


Assessment of cellular immunity by 51Cr release from macrophages as target cells.


Letter: Absence of serologically detectable H-2 on primitive teratocarcinoma cells in culture.


Construction and use of a partograph in the management of labour.


Treatment of the suicidal character.

Treatment of the suicidal character.


The transsexual syndrome in males. II. Secondary transsexualism.


Morita therapy.

Morita therapy.


Conflict, negotiation, and cooperation: an analysis of these parameters in national and international relations.


Psychotherapeutic interaction.

Psychotherapeutic interaction.


Transcendence and psychotherapy.

Transcendence and psychotherapy.


The paper-writing function of the ego.

The paper-writing function of the ego.


Three perspectives on brief psychotherapy.


The vicissitudes of discretion in psychotherapy.


Musical associations in psychotherapy.

Musical associations in psychotherapy.


Therapeutic application of a simple relaxation method.


A surgical perspective of mammography and thermography.


Unusual diagnostic aspects and current management of pancreatic pseudocysts.


Patients with hyperbilirubinemia and hyperamylasemia: is the diagnosis pancreatitis or biliary lithiasis?


Left ventriculoaortic shunting: a laboratory investigation.


Congenital duodenal web in an adult.

Congenital duodenal web in an adult.


Early management of vesicointestinal malformations in the newborn.


Acute diverticulitis of the cecum.

Acute diverticulitis of the cecum.


A rigid dressing with a pneumatic prosthesis for below-knee amputees.


Incarcerated Bochdalek hernia in the adult: report of a case.


Solitary diverticulum of the colon.

Solitary diverticulum of the colon.


Editorial: An arthurian legend: oxygen and the regulation of blood flow.


Oxygen transport during acute alkalosis and hyperphosphatemia in dogs.


Renal effects and metabolism of isoflurane in man.


The effect of unilateral pulmonary-artery occlusion on the rate of increase of arterial anesthetic concentration.


Is depression of mitochondrial respiration a predictor of in-vivo anesthetic activity?


The effects of isoflurane on canine cerebral metabolism and blood flow.


Canine cerebral oxygen consumption during enflurane anesthesia and its modification during induced seizures.


Cardiovascular manifestations of tetanus.


Physiologic deadspace-to-tidal volume ratio: a new working equation and nomograms.


A complication of radial-artery cannulation.


A comparison of the effects of intravenous innovar and topical spray on the laryngeal closure reflex.


Anesthetic management of cholecystectomy in a patient with buccal pemphigus.


The use of epsilon-aminocaproic acid to reduce bleeding during cardiac bypass in children with congenital heart disease.


Letter: Investigating acupuncture.

Letter: Investigating acupuncture.


Letter: CO2 absorber placement affects spirometric measurements of gas exchange and pulmonary function.


Letter: Anesthesiologists in intensive care.


Sequence of degeneration of Corti's organ and its first-order neurons.


The concentration of antibiotics in sinus secretions. Ampicillin, cephradine and erythromycinestolate.


Electrocochleography.

Electrocochleography.


Inherited Bell's palsy.

Inherited Bell's palsy.


Pelvic anatomy. I. Pelvic floor muscles.


Diagnosis and management of acute pancreatitis.


Ectopic salivary gland and sinus in the lower neck.


Diuretic and diet treatment of hypertension.


Letter: Iron deficiency and thrombocytosis.


Automated "chemistries". A multiphasic misadventure.


Letter: Hemochromatosis.

Letter: Hemochromatosis.


A new silicone elastomer tube for use during microsurgery on the larynx.


Localization of 99mtechnetium in the region of the nose in Sjögren's syndrome.


Origins of the crossed olivocochlear bundle shown by an acid phosphatase method in the cat.


Immunoglobulin E (IgE) in middle ear effusions.


Hypertension--1974.


The clinical spectrum of essential hypertension.


Renal and renovascular hypertension. A reasoned approach to diagnosis and management.


Effects of age, sex, and smoking on electrical taste threshold.


A contribution to the physiology of the perilymph. 3. On the origin of noise-induced hearing loss.


Preventing decalcification of human ossicles stored in a buffered formaldehyde solution.


Intranasal meningoencephalocele.

Intranasal meningoencephalocele.


Patulous eustachian tube syndrome. Preliminary report of the use of the tensor veli palatini transposition procedure.


Acupuncture for hearing loss. A study of patients treated in Washington, DC.


Phase distortion of averaged electroencephalic response.


Postoperative and postradiotherapeutic serous otitis media.


Rupture of the round window membrane in inner ear barotrauma.


Hemangioma of bone.

Hemangioma of bone.


Cochleoneural hearing loss treated with acupuncture.


Ocular hypertension following use of an antral balloon.


Silicone rubber stent. An improved form of ear packing.


Pathologic quiz case 1.

Pathologic quiz case 1.


Pathologic quiz case 2.

Pathologic quiz case 2.


Pathologic features of enteric infection with Yersinia enterocolitica.


Ultrastructure of human fibroadenoma.

Ultrastructure of human fibroadenoma.


Testicular biopsy in the study of male infertility. 3. Pretesticular causes of infertility.


Weight of the spleen. I. Range of normal in a nonhospital population.


Weight of the spleen. II. Range in narcotic addicts.


Amnion nodosum: ultrastructure and histopathogenesis.


Type 3 hyperlipoproteinemia with mesangial foam cells in renal glomeruli.


Severe pulmonary atherosclerosis in hypercholesterolemic rabbits.


Ochronotic arthropathy: an electron microscopical study with a view on pathogenesis.


Secretion of granule content by eosinophils.


Letter: Adrenal leukodystrophy.

Letter: Adrenal leukodystrophy.


Primary leiomyosarcoma of the heart: report of a case and review of the literature.


Letter: Eye registry for the pathologist and ophthalmologist.


Letter: Chondrosarcoma.

Letter: Chondrosarcoma.


Surgical manpower. A symposium.

Surgical manpower. A symposium.


Nonatherosclerotic segmental arterial occlusion of the extremity.


Vascular compression of the duodenum and peptic ulcer disease.


On the corrosive properties of bile and pancreatic juice on living tissue in dogs.


Fracture healing. Compression vs fixation.


Effect of vagotomy on hormones stimulating pancreatic secretion.


Hemodynamic response to vasodilation and exercise in "critical" arterial stenosis. An experimental study in a canine model.


Hepatic ascariasis. A case report and review of the literature.


Mediastinal hemorrhage from parathyroid adenoma simulating dissecting aneurysm.


Small bowel polyps and carcinoma in multiple intestinal polyposis.


High-speed pneumatic drill for biopsy of thyroid lesions.


The air splint. An alternative to the immediate postoperative prosthesis.


Letter: Autotransfusion in three variations.


Letter: Enterolith small bowel obstruction (pseudogallstone ileus).


Editorial: Hemorrhoids.

Editorial: Hemorrhoids.


Editorial: Autotransfusion--safe at any speed?


Full-time or geographic?

Full-time or geographic?


External carotid artery shunting during carotid endarterectomy. Evidence for feasibility.


The placement of vena cava umbrella filters. The value of phlebography.


Sudden complete thrombosis of aortic and iliac aneurysms.


Massive pulmonary hemorrhagic infarction.


Sclerosis of the extrahepatic bile ducts.


Acute aortic dissection during cardiopulmonary bypass. Successful treatment of three patients.


Rapid and accurate preoperative diagnosis of obstructive jaundice. Utilization of visceral angiography and transhepatic cholangiography.


Penetrating injuries of the abdominal aorta.


Hematologic integrity after intraoperative allotransfusion. Comparison with bank blood.


Role of proteolytic enzyme in the prevention of postoperative intraperitoneal adhesions.


Reversal of colonic net absorption to net secretion with increased intraluminal pressure.


Traumatic aneurysm of the abdominal aorta. Report of two cases and review of the literature.


Chylopericardium as a complication of aortopulmonary shunt.


Acute appendicitis secondary to metastatic carcinoma of the breast. A report and review of two cases.


Use of an oral-anal string as a guideline for colonoscopy.


Letter: Abscess in nonlactating breast.

Letter: Abscess in nonlactating breast.


Letter: Perforated diverticulitis of the transverse colon.


Ultrastructural investigation of human atrial myocardium in cardiac malformation with special regard to z-band alterations.


Breakdown of the nuclear envelope in hen erythrocytes after phenylhydrazine treatment.


Liver morphology in a case of citrullinemia (a light and electron microscopic study).


Pressure monitor isolating devices for haemodialysis--reducing the hepatitis risk.


A photon absorptiometer for in vivo and in vitro measurement of small animal bone density.


The importance of constancy of period in checking implanted pacemakers.


A hybrid servo system for adjustable, linear scanning.


Letter: Electrical safety of ECG writers.


A semi-automatic oil spreader for ultrasonic B-scanning.


Chronic exposure to benzene as a possible contributary etiologic factor in Hodgkin's disease.


Editorial: Jaundice after halothane.

Editorial: Jaundice after halothane.


Editorial: Biopsy in alcoholic liver disease.


Letter: Hospital admission x-rays.

Letter: Hospital admission x-rays.


Letter: Diagnostic value of reviewing old chest x-rays.


Letter: Duplication of the alimentary tract.


Letter: Antiasthmatic effect of amitriptyline.


Letter: Treatment of early breast carcinoma in northern Alberta--a follow-up study.


Letter: Hepatitis B in pregnancy.

Letter: Hepatitis B in pregnancy.


Letter: Hazards of bunk beds.

Letter: Hazards of bunk beds.


Letter: Saccharin and carcinoma of the bladder.


The late results of cardiac valve replacement using autologous fascia lata. Over an eight-month period beginning in November 1969, 53 patients received 63 fascia lata heart valves at the Toronto General Hospital. The late results of this form of valve substitution are reviewed. The fascia used to fashion the tricuspid valve underwent progressive thickening and contracture and this process caused failure of the prosthesis within months of insertion. The mitral fascial prosthesis failed in a similar manner although the process took longer. The aortic fascia lata valve, however, has not shown progressive thickening after 3(1/2) years and it has so far retained its functional integrity. Indeed, we have been impressed by the excellent clinical results and absence of complications such as thromboembolism although anticoagulation has not been used. We therefore consider that fascia lata valves offer a suitable alternative to other forms of aortic valve substitution, but are unsatisfactory for tricuspid or mitral valve replacement.


Heparin treatment in abruptio placentae. Two cases of abruptio placentae with disseminated intravascular coagulation (DIC) were treated with heparin, and coagulation was monitored by thromboelastography as well as the usual hematology tests. The cases demonstrated the vagaries of DIC and both showed decreased overt hemorrhage after heparin treatment was started. Heparin may be indicated for the management of abruptio placentae where delivery is not imminent, where significant disseminated intravascular coagulation exists, and when adequate serial coagulation studies are available.


The dimensions of sports medicine.

The dimensions of sports medicine. The history of sports medicine is reviewed in terms of European, United States and Canadian traditions. An orientation towards treatment, particularly the treatment of injuries in elite athletes, is noted. Encouragement is found in the trend to discussion between the several Canadian groups interested in sports medicine. It is suggested that in the leisured society of the future, sports medicine will form an increasing part of family practice. It is to be hoped that emphasis will be placed on a broad-based approach to prevention of accidents and disease, coupled with the effective promotion of positive health measures, particularly increased physical activity.


A case of Powassan virus encephalitis.

A case of Powassan virus encephalitis. A case of encephalitis due to Powassan virus, probably transmitted through tick-bite, is reported in an 8-year-old boy. There was a 50-fold increase in neutralization titre against Powassan virus, but the virus could not be isolated. Other virological investigations were negative.The patient survived and early physiotherapy and speech re-education could be instituted. Nine months after onset of illness the patient showed moderate sequelae, despite a very severe illness.


Canadian Council on Hospital Accreditation. IV. What's ahead for the council?


Introducing a feature aimed at producing meaningful dialogue between physician and patient.


Lay involvement in health care.

Lay involvement in health care.


Basic physical assessment.

Basic physical assessment.


Behavioral effects of dialysis.

Behavioral effects of dialysis.


Wanted: a definition of nursing practice.


An experimental nursing curriculum.

An experimental nursing curriculum.


A self-help clinic for women.

A self-help clinic for women.


Proceedings: Abnormal sodium transport in leucocytes of patients with essential hypertension.


Proceedings: Complement activation following myocardial infarction.


Proceedings: Long-term changes in myocardial blood flow following saphenous vein grafting of coronary arteries.


Proceedings: Reticulo-endothelial clearance studies in the course of horse serum induced nephritis.


Proceedings: Amine metabolism and the small intestinal flora in uraemia.


Proceedings: Plasma protein synthesis in isolated hepatocyte cultures.


The mechanisms by which sodium excretion is increased during a fast but reduced on subsequent carbohydrate feeding.


Total body, plasma and erythrocyte potassium and leucocyte ascorbic acid in 'ultra-fit' subjects.


Comparison of the renin response to dopamine and noradrenaline in normal subjects and patients with autonomic insufficiency.


The effect of aldosterone on colonic potential difference and renal electrolyte excretion in normal man.


Hepatic protein binding of folate.

Hepatic protein binding of folate.


Isoenzyme studies in the identification of transplanted muscle in the mouse.


Plasma amino acids in experimental acute hepatic failure and their relationship to brain tryptophan.


Plasma and hepatic cobalamins in tropical ataxic neuropathy.


Somatomedin.

Somatomedin.


Pituitary-ovarian interrelationships in the rat.


Distribution of plasma amino acids in humans during submaximal prolonged exercise.


An improved method of quadriceps thermocouple implantation.


Characteristics of sprint, medium and long-distance swimmers.


Association serves 17 hospitals.

Association serves 17 hospitals.


Wide-range sharing for two hospitals.

Wide-range sharing for two hospitals.


Law in brief: abortion: the unanswered questions.


Editorial: Collaborative systems--realistic and opportune.


Status of multihospital systems.

Status of multihospital systems.


Systems for collaborative delivery of health care: governance seen as weak link.


Hospitals can and do cooperate.

Hospitals can and do cooperate.


Hospital and group practice form HMO.

Hospital and group practice form HMO.


HMOs don't just happen.

HMOs don't just happen.


Human concerns about hospital mergers.

Human concerns about hospital mergers.


Systems for collaborative delivery of health care: consortium achieves mutual goals.


Thalidomide and beyond: some recommendations regarding public policies toward the ethical drug industry.


Nonprescription drugs: an overview.

Nonprescription drugs: an overview.


Drug benefits in health insurance.

Drug benefits in health insurance.


Public policy considerations in the pricing of prescription drugs in the United States.


Pharmacy manpower in the United States: a wasted resource.


Medical interests and the public interest: a comparison of West Germany and the United States.


How can the U.S. government control physicians' fees under national health insurance? A lesson from the French system.


Special section on analysis and evaluation of the drug industry. Introduction to the theme: the role of the drug industry in modern health services systems.


Iatrogenic disease: an epidemiologic perspective.


The drug regulatory system of the United States Food and Drug Administration: a defense of current requirements for safety and efficacy.


Mechanical aspects of rowing.

Mechanical aspects of rowing.


Contribution of airway collapse to supramaximal expiratory flows.


Distribution of ammonia between blood and cerebrospinal fluid in pulmonary emphysema.


Increased thermal body insulation: relationship to the development of obesity.


Sinoaortic contribution to the adjustment of systemic resistance in exercising dogs.


Instantaneous dimensional changes of the right atrium of the cat.


Effects of mixing patterns in respiratory gas exchange.


Accessibility of pulmonary stretch receptors from the pulmonary and bronchial circulations.


Maximum oxygen uptake during swimming and running by elite swimmers.


Intracellular free amino acid concentration in human muscle tissue.


Direct action of contracting diaphragm on the rib cage in rabbits and dogs.


A comparison of three maximal treadmill exercise protocols.


Control of skin blood flow, sweating, and heart rate: role of skin vs. core temperature.


Effects of simulated weightlessness on responses of untrained men to +Gz acceleration.


Flow limitation during forced expiration in excised human lungs.


Specificity of cardiorespiratory adaptation to bicycle and treadmill training.


Maximal oxygen uptake based on lean body mass: a meaningful measure of physical fitness?


Recording of multichannel analog data and video images on the same video tape or disc.


Solenoid-operated valve box providing sudden undetectable changes of inspired gas.


A new indwelling catheter for coronary sinus blood sampling in dogs.


Electrical gradients across an ion-exchange membrane in student's artificial cell.


Bacterial bioluminescence in vivo: control and synthesis of aldehyde factor in temperature-conditional luminescence mutants. Bioluminescent marine bacteria possess luciferase, which catalyzes the oxidation of reduced flavin mononucleotide and long-chain aldehyde to produce light. Temperature-sensitive mutants of these bacteria can be obtained which require exogenous aldehyde for light production at higher temperatures. In Beneckea harveyi. two classes of such mutants were found which differed with regard to their response to temperature shifts. In one class, a shift from permissive to nonpermissive temperature in liquid cultures resulted in a rapid (t((1/2)) approximately 3 min) loss of luminescence. In the other, there was no immediate decline in luminescence; it was the increase of luminescence that was blocked. Through studies of these and other effects of temperature shifts on the in vivo luminescence of these mutants, we conclude that at least two genes are specifically involved in the in vivo biosynthesis of aldehyde for the luminescence reaction and that both genes are coordinately controlled with that for luciferase.


Inhibition of the development of competence in Streptococcus sanguis (Wicky) by reagents that interact with sulfhydryl groups: discernment of the competence process. Reagents that interact with sulfhydryl groups are shown to inhibit competence factor (CF)-induced competence development in Streptococcus sanguis (Wicky) strain WE4 (Wicky 4 Ery(R)). Inhibition is correlated with specific inhibition of either the function or biosynthesis of three competent cell-related proteins and is reversed by either 2-mercaptoethanol or dithiothreitol. Mercuric chloride (5 muM) or N-ethylmaleimide (NEM; 50 muM) inhibited (i) the function but not the biosynthesis or activation of the competent cell-associated autolysin; (ii) the biosynthesis of a competent cell-associated protein of unknown function, demonstrated by polyacrylamide gel electrophoresis of acidified phenol extracts; and (iii) the biosynthesis or activation of distinct deoxyribonucleic acid (DNA)-binding sites. Neither reagent at the indicated concentration interfered with the uptake of CF by cells or with the uptake and expression of DNA by competent cells. Neither reagent inactivated CF or genetic markers coded by the transforming DNA, nor did they inhibit cell growth or viability appreciably. The data reveal that either mercuric chloride or NEM can differentially inhibit induced protein synthesis and, in addition, conclusively show that some autolytic activity is essential for the onset of the competent state.


Intracellular localization of nigeran in the wall of Aspergillus aculeatus by autoradiography with the electron microscope. Aspergillus aculeatus mycelium was incubated with [(3)H]glucose under conditions that promote nigeran (mycodextran) accumulation (low pH, in the absence of nitrogen). Autoradiography revealed that essentially all the (3)H was localized around the hyphal perimeter. The results strongly support a hyphal wall location for nigeran.


Dissociation of a degradative plasmid aggregate in Pseudomonas. The infectious plasmid OCT, which specifies a set of dissimilatory enzymes responsible for the degradation of n-octane, has been shown to be an aggregate of a noninfectious OCT plasmid and an infectious plasmid with sex factor activity. The infectious plasmid, which can be eliminated from the cells of Pseudomonas putida by mitomycin C treatment without loss of the OCT plasmid and vice versa, has been designated as factor K. The infectious plasmid (factor K) is not only responsible for the mobilization of OCT, but also mobilizes chromosomal genes at a frequency of 10(-2) to 10(-3) per donor cell. Whereas OCT is incompatible with another degradative plasmid, CAM, factor K appears to be compatible with it.


Effect of competence induction on macromolecular synthesis in a group H streptococcus. The effects of competence induction by competence factor (CF) on macromolecular synthesis in group H streptococcus strain Wicky were investigated. CF preparations (culture filtrates from competent group H streptococcus strain Challis) were either heated or partially purified to remove a bacteriocin. These preparations did not inhibit growth, although they induced high levels of competence in strain Wicky. The action of the CF preparations did not affect the overall rates of deoxyribonucleic acid and protein synthesis, but caused a reduction in the rates of ribonucleic acid (RNA) and peptidoglycan synthesis. When competence induction by CF was prevented, no alterations in RNA or peptidoglycan synthesis were observed, indicating that these changes are in fact related to the development of competence.


Effect of substrate on the fatty acid composition of hydrocarbon-utilizing filamentous fungi. The fatty acid pattern in hydrocarbon-utilizing filamentous fungi was determined after growth on acetate, propionate, n-alkanes (C(13) to C(15)), and alk-1-enes (C(14) to C(18)). The fatty acid profile of Cunninghamella elegans and Penicillium zonatum after growth on acetate shows a predominance of even-carbon fatty acids (C(16), C(18:1), C(18:2)), whereas cells grown on propionate showed significantly higher levels of odd-carbon fatty acids (C(15), C(17), C(17:1)). Growth on n-alkanes resulted in the incorporation of fatty acids homologous to the growth substrate. Cunninghamella elegans grown on the alk-1-enes from C(14) to C(18) incorporated the unsaturated substrate into cellular fatty acid after oxidation at the saturated end of the molecule. Regardless of substrate these fungi contain, predominantly, fatty acids 18 carbons in length.


Chemical and physical properties of lipopolysaccharide of Yersinia pestis. Lipopolysaccharide (LPS) prepared from Yersinia pestis 195/P contained d-glucose, d-glycero-d-mannoheptose, l-glycero-d-mannoheptose, glucosamine, 3-deoxyoctulosonic acid, lipid A, beta-hydroxymyristate, acetyl, phosphate, and protein. Traces of ethanolamine, mannose, and galactose were also detected. The lipid A moiety was composed of glucosamine substituted with phosphate, amide-linked beta-hydroxymyristate, and amide-bound acetate. The absence of significant amounts of additional fatty acids indicates a lipid A structure somewhat less complex than that of other gram-negative bacteria. The sugars identified are those generally found in the "core" region of LPS from the Enterobacteriaceae, with the exception of the d-glycero-d-mannoheptose. The molecular weight of the aggregated LPS was estimated to be 1.6 x 10(8).


Purification and properties of undecyl acetate esterase from Pseudomonas cepacia grown on 2-tridecanone. Undecyl acetate esterase has been purified from Pseudomonas cepacia grown on the methyl ketone, 2-tridecanone. The K(m) for undecyl acetate was 2.3 x 10(-2) M. Polyacrylamide gel electrophoresis indicated that two esterase bands were being recovered during purification. These bands were separated by preparative polyacrylamide gel electrophoresis. Molecular weights were estimated to be approximately 34,500 by several methods. Molecular sieve polyacrylamide gel electrophoresis indicated that the two esterases had the same molecular weight but different charge, which is indicative of isoenzymes.


Heptose-containing pentaglycosyl diglyceride among the lipids of Acholeplasma modicum. A pentaglycosyl diglyceride with the tentative structure of galactosyl-galactosyl-mannoheptosyl-glucosyl-glucosyl diglyceride was found to be the major glycolipid in Acholeplasma modicum. The heptose is d-glycero-d-mannoheputose. The diglyceride-terminating moiety possesses the structure O-alpha-d-glucopyranosyl-(1 --> 2)-O-alpha-d-glucopyranosyl-sn-1,2-diglyceride. Other glycolipids occurring in this organism are a diglucosyl diglyceride and a monoglucosyl diglyceride with structures identical to the terminal segments of the pentaglycosyl diglyceride. More fully acylated derivatives of these two glycolipids also occur. The phospholipids are all of the glycerophosphoryl type. The neutral lipids are composed of diglycerides and four polyterpenes. The polyterpenes consist of both colored and colorless carotenoids and become radiolabeled with both [(14)C]acetate and [(14)C]mevalonate.


Distribution of the isopropylmalate pathway to leucine among diverse bacteria. alpha-Isopropylmalate synthase and beta-isopropylmalate dehydrogenase activities were detected in extracts of the following organisms: Chromatium D, Rhodopseudomonas spheroides, Hydrogenomonas H16, Pseudomonas aeruginosa, Pseudomonas fluorescens, Vibrio extorquens, Rhizobium japonicum, Alcaligenes viscolactis, Escherichia coli B, Proteus vulgaris, Aerobacter aerogenes, Salmonella typhimurium, Micrococcus sp., Micrococcus lysodeikticus, Bacillus polymyxa, Bacillus subtilis, and Nocardia opaca. The alpha-isopropylmalate synthase activity in these extracts was inhibited by low concentrations of l-leucine. Taken together with other data, these results suggest that the isopropylmalate pathway is widespread among organisms that can synthesize leucine.


Effect of ethidium bromide on elimination of exfoliative toxin and bacteriocin production in Staphylococcus aureus. The scalded skin syndrome has been associated with phage group II staphylococci. The clinical manifestations of scalded skin syndrome, Ritter's disease, scarlatiniform erythema, and localized bullous impetigo, are due to the production of an extracellular protein, designated exfoliative toxin. Phage group II staphylococci can also produce an extracellular protein, bacteriocin, which is bacteriocidal for specific gram-positive microorganisms. Strain UT 0007 produces both bacteriocin and exfoliative toxin which appear to be products of extrachromosomal genes. These genes are jointly eliminated from strain UT 0007 after growth in either ethidium bromide or at high temperatures.


Demonstration and characterization of two distinct human leukocytic pyrogens. Human monocytes and neutrophils were separated from buffy coats of blood obtained from normal donors. Following incubation with heat-killed staphylococci, monocyte preparations contained 20 times more pyrogenic activity in the supernatant media than did supernates from an equal number of neutrophils. During purification of these pyrogens it was discovered that these cell preparations each produced a distinct and different pyrogen. The pyrogen obtained from neutrophils had a mol wt of 15,000 following Sephadex G-75 gel filtration, an isoelectric point of 6.9, and could be precipitated and recovered from 50% ethanol at -10 degrees C. In contrast, the pyrogen derived from monocyte preparations had a mol wt of 38,000, an isoelectric point of 5.1, and was destroyed in cold ethanol. Both molecules were unaffected by viral neuraminidase but biologically destroyed at 80 degrees C for 20 min and with trypsin at pH 8.0. The febrile peak produced by partially purified neutrophil pyrogen occurred at 40 min while that from monocytes was at 60 min. In addition, monocyte pyrogen produced more sustained fevers for the same peak elevation as neutrophil pyrogen. These studies demonstrate for the first time two chemically and biologically distinctive pyrogens derived from circulating human white blood cells and have important implications for our understanding of the pathogenesis of fever in man.


Substituted azopurines. 1. Synthesis of 8,8'-dioxo-6,6'-azopurine.


Aminobenzoic acid diuretics. 6. 4-Substituted 3-alkylthio-5-sulfamoylbenzoic and 5-sulfamoylthiosalicylic acids.


Ab initio calculations on large molecules using molecular fragments. Lincomycin model studies.


Cell surface immunoglobulin. IX. A new method for the study of synthesis, intracellular transport, and exteriorization in murine splenocytes. A new method for the detection of cell surface immunoglobulin labeled with isotopic precursors is described. The method consists of the aggregation of surface Ig on cells with specific antibody (heterologous) and the subsequent removal of antigen-antibody complexes by the combination of high speed centrifugation and immunoprecipitation of remaining soluble complexes using antibody to the heterologous Ig. Using this method, the kinetics of appearance of cell surface Ig and its turnover were studied in murine splenocytes. The results suggest that cell surface Ig is synthesized and transported in the same manner as secretory Ig rather than being synthesized on the plasma membrane. The turnover of intracellular and cell surface Ig in lymphocytes is slow. In contrast, intracellular Ig in plasma cells is rapidly secreted and usually without a cell surface phase. Cell surface Ig was shown to be radiolabeled with [(3)H]glucosamine, -galactose, and -fucose. The proportion of cell surface to intracellular (nonsurface) Ig labeled with these precursors suggests the same sequence of addition of sugars to Ig destined to be on the surface of lymphocytes as with Ig which will be secreted by plasma cells. Results with this new method also confirm earlier conclusions based on experiments using cell surface iodination: 8S IgM is the predominant Ig on the surface of murine splenocytes and the molecule appears to be attached by its micro-chains.


Ab initio calculations on large molecules using molecular fragments. Structural correlations between natural substrate moieties and some antibiotic inhibitors of peptidyl transferase.


Microbial transformations of antitumor compounds. 1. Conversion of acronycine to 9-hydroxyacronycine by Cunninghamella echinulata.


Synthesis and pharmacological evaluation of 2,3-dihydro-1H-thieno(2,3-e)(1,4)diazepines.


Adamantanealkanamines as potential antidepressant and anti-Parkinson agents.


Synthesis and antiinflammatory activity of some 1-alkyl-4-phenylpyrido(2,3-d)pyrimidin-2(1H)-ones.


Fibrin-stabilizing factor inhibitors. 11. Monodansylated weak aliphatic diamines.


4-(and 5-) (2-Thienyl)-1-naphthaleneacetic acids as antiinflammatory agents.


6-phenyl-6-propionoxyperhydrobenzo(c)quinolizines as potential analgetics.


Scorpion toxin-induced catecholamine release from synaptosomes.


Total synthesis of the antitumor antibiotic danuorubicin. Coupling of the sugar and aglycone.


Protein synthesis by cerebral polysomes pretreated with puromycin.


Galactosylsphingosine galactosyl hydrolase in rat brain: probable identity with galactosylceramide galactosyl hydrolase.


Axonal transport of (3H)glucose radioactivity in the optic system of Scardinius erythrophthalamus.


The fatty acid composition of sphingolipids from bovine CNS axons and myelin.


Dopamine in the mesolimbic system of the rat brain: endogenous levels and the effects of drugs on the uptake mechanism and stimulation of adenylate cyclase activity.


Anomalies of myelin-associated glycoproteins in 'Quaking' mice.


Serum cerebrosides in multiple sclerosis.


Brain amino acids during convulsions.

Brain amino acids during convulsions.


On the relationship of brain filaments to microtubules.


Selective retention and filtration of brain nucleic acids in agarose gels.


Cerebrospinal fluid homovanillic acid and iso-homovanillic acid: a gas-liquid chromatographic method.


The effects of lithium on high energy phosphate and glucose levels in the rat superior cervical ganglion.


Local changes in subcellular distribution of dopamine-beta-hydroxylase (EC 1.14.2.1) after blockade of axonal transport.


Spinal subarachnoid perfusion in the rat: glycine transport from spinal fluid.


Lipid composition in gray and white matter of the brain in Menkes' disease.


Influence of hypoxia on cerebral energy state in rats with porta-caval anastomosis.


Effect of electrical stimulation on the yield and composition of synaptic vesicles from the cholinergic synapses of the electric organ of Torpedo: a combined biochemical, electrophysiological and morphological study.


Changes in the development of central noradrenaline neurones following neonatal administration of 6-hydroxydopamine.


Soluble and particulate hexokinase in developing neural systems.


Protein synthesis and nerve cell specificity.


Utilization of acetate and pyruvate for the synthesis of 'total', 'bound' and 'free' acetylcholine in the electric organ of Torpedo.


The influence of functional alteration on monoamine oxidase and catechol-O-methyl transferase in the visual pathway of rats.


Flow in vivo of glucose carbon to brain protein in rats: effect of starvation.


Effect of undernutrition on amino acid compartimentation in the developing rat brain.


Neonatal undernutrition and RNA synthesis in developing rat brain.


Depletion of tryptophan hydroxylase by 5,6-dihydroxytryptamine in rat brain. Time course and regional differences.


Serotonin and free amino acid analysis of ganglia and isolated neurones of Aplysia dactylomela.


Fluid spaces of synaptosome beds.

Fluid spaces of synaptosome beds.


The mechanism of the exclusion of sodium ions and the concentration of potassium ions by guinea-pig cerebral cortex slices.


Effect of electroconvulsive therapy on plasma unesterified fatty acid and free tryptophan concentrations in man.


Dopamine in an identified neuron of Planorbus corneus.


Carboxypeptidase of human brain: hydrolysis of benzyloxycarbonyl-glutamyl-Ltyrosine in normal and diseased tissue.


Amino-oxyacetic acid: a relatively non-specific inhibitor of uptake of amino acids and amines by brain and spinal cord.


Inhibition of acetylcholine synthesis in neuroblastoma cells by a styrylpyridine analog.


Letter: Cell as receptor system.

Letter: Cell as receptor system.


Influence of hydrophilic-lipophilic balance values of surfactants on ephedrine absorption and release from emulsified systems after oral administration to dogs.


Surfactant effects on drug absorption. 3. Effects of sodium glycocholate and its mixtures with synthetic surfactants on absorption of thaimine disulfide compounds in rat.


Drug permeation through membranes. 3. Effect of pH and various substances on permeation of phenylbutazone through everted rat intestine and polydimethylsiloxane.


Effects of alkyl chain length on biological activity: alkyl p-aminobenzoate-induced narcosis in goldfish.


Theoretical model studies of intestinal drug absorption. IV. Bile acid transport at premicellar concentrations across diffusion layer-membrane barrier.


Titration of acids and acid mixtures in benzene-acetonitrile binary solvent system.


Mechanism of histimine binding. I. Effect of calcium on binding of histamine to a plasma peptide.


Bioavailability of aminosalicylic acid and its various salts in humans. 3. Absorption from tablets.


Hydrolysis kinetics of benzocaine and homologs in the presence of a nonionic surfactant.


Hydrolysis and epimerization kinetics of pilocarpine in aqueous solution.


Corneal absorption of ophthalmic drugs.

Corneal absorption of ophthalmic drugs.


Influence of shape factors on kinetics of drug release from matrix tablets. I. Theoretical.


Influence of shape factors on kinetics of drug release from matrix tablets. II. Experimental.


Improved methods for quantitative determination of methadone.


Topochemical decomposition patterns of aspirin.


Sterilization of selected colorants by cobalt-60.


Analysis of sodium levothyroxine or sodium liothyronine in tablets.


Dissolution of lithium and magnesium from lithium carbonate capsules containing magnesium stearate.


Log-normal distribution of tablet pore diameters.


Integral approach to nonisothermal estimation of activation energies.


Quantitative GLC analysis of plasma cholesterol.


Correlation of plasma ibuprofen levels with biological activity.


Effect of bile salts on partitioning and oral toxicity of the bisquaternary ammonium drug decamethonium bromide.


Effects of dimethyl sulfoxide and trimethylphosphine oxide on percutaneous absorption of corticosteroids in the rat.


Thermal stability of biological lipids. I. Differential scanning calorimetry of mixtures of cholesteryl myristate and cholesteryl palmitate.


Prodrug approach to enhancement of rate of dissolution of allopurinol.


Separation of prostaglandin A2 and prostaglandin B2 by ion-exchange liquid chromatography. 2 closely related prostaglandins (PGs), A2 and B2, were separated by ion-exchange liquid chromatography.  PGA2 and PGB2 are an isomeric pair of PGs which show little resolution on thin-layer chromatography; the pair can be resolved as gas-liquid chromatography, but use of this method requires protection of the C9-carbonyl group.  Ion-exchange liquid chromatography, in contrast, requires no protective derivatization to achieve separation of PGA2 and PGB2 from PGE2.  2 different column types were used, and their results compared favorably.  In 1 experiment, a triethylaminoethyl cellulose column resolved the 2 species; in another experiment, a strong anion-exchange pellicular support was successful.  Both columns were stable and could be used to monitor the stability of PGE2 by following the appearance of PGA2 and PGB2, using the peak height method (i.e., a plot of micrograms injected vs. peak height).  Triethylaminoethyl cellulose gave somewhat better resolution of PGA2 and PGB2 as compared with the pellicular strong anion-exchange column, but the advantage of this effect is offset by the tediousness of packing columns of triethylaminoethyl cellulose.


Effects of gamma-irradiation on 2-chloroethanol formation in ethylene oxide-sterilized polyvinyl chloride.


Letter: Effect of plasma protein binding on elimination of warfarin.


Letter: GLC microdetermination of plasma anticonvulsant levels.


Letter: Enhancement of optical rotation of levodopa by cyclization.


Letter: Slide test as a micromethod of a modified Limulus endotoxin test.


Letter: Rapidly dissolving forms of digoxin: hydroquinone complex.


Essentials of physical management and rehabilitation in arthritis.


Safety of intraaortic balloon pumping. II. Physical injury to aortic endothelium due to mechanical pump action.


Influence of blood flow velocity on experimental haemostatic plug formation.


Inhibition of platelet aggregation by medium-chain fatty acids.


Letter: The fibrinogenolytic pathway of fibrinogen catabolism: a reply.


Comparsion of the effect of intraportal and intravenous infusion of insulin on blood glucose and free fatty acids in peripheral venous blood of man.


Effects of dopamine on hemodynamics and renal function.


Radioisotope renography, glomerular filtration rate and effective renal plasma flow in donors with normal renal function after nephrectomy.


Relationship between blood pressure and physical fitness, smoking and alcohol consumption in Copenhagen males aged 40-59.


Urinary growth hormone in acromegaly.

Urinary growth hormone in acromegaly.


The prognosis for patients with complete heart block treated with permanent pacemaker.


Acute myocardial infarction complicated by third degree atrioventricular block treated with temporary pacemaker. Hospital and long-term survival in 57 patients.


The accuracy of auscultatory measurement of arm blood pressure in very obese subjects.


Bumetanide in the treatment of hepatic ascites. A short and long-term study.


Penicillamine treatment of cystinuria.

Penicillamine treatment of cystinuria.


Cortisol and cortisone acetate in parenteral glucocorticoid therapy?


Pericarditis following cardioversion. A case report.


Insulin deficiency associated with hypoglycemia and good glucose tolerance in hypopituitarism. Report of a case with acromegaly developing pituitary apoplexy.


Properties of a complex of iron and a sorbitol-gluconic acid polymer for treatment of piglet anaemia.


The course of an experimental liver fluke infection in the pig.


A low-selenium pig diet based on casein from selenium-deficient cows.


Method for the replacement of a permanently implanted arterial catheter without surgery.


Studies on Fasciola hepatica miracidia labelled with radioselenium.


Osteochondrosis and arthrosis in pigs. I. Incidence in animals up to 120 kg live weight.


Osteochondrosis and arthrosis in pigs. II. Incidence in breeding animals.


Osteochondrosis and arthrosis in pigs. 3. A comparison of the incidence in young animals of the Norwegian Landrace and Yorkshire breeds.


Osteochondrosis and arthrosis in pigs. IV. Effect of overloading on the distal epiphyseal plate of the ulna.


Osteochondrosis and arthrosis in pigs. V. A comparison of the incidence in three different lines of the Norwegian Landrace breed.


Diurnal variation of peripheral plasma levels of testosterone in bulls measured by a rapid radioimmunoassay procedure.


Treating acute hand and finger injuries.


Horner's syndrome.

Horner's syndrome.


The extra heart sound.

The extra heart sound.


Vitamin nutriture in black, urban, day care children.


Relationship of dietary tryptophan and niacin to tryptophan metabolism in schizophrenics and nonschizophrenics.


Relationship of dietary tryptophan and niacin to tryptophan metabolism in alcoholics and nonalcoholics.


Survey of vitamin B12 and folate in the serum and marrow tissue of hospitalized patients.


Dietary fluoride in different areas in the United States.


The importance of careful examination of ocular motility and its clinical significance.


Prognosis in pleoptic therapy.

Prognosis in pleoptic therapy.


Introduction to low vision aids.

Introduction to low vision aids.


Velocity measurements as dynamic parameters for ocular studies.


Acute loss of fusional convergence following head trauma.


Keeping that patch on: the application of behavior modification techniques in orthoptic practice.


Orthoptic management of intermittent exotropia.


Ocular malingering.

Ocular malingering.


Measurement of the field of binocular single vision in the evaluation of incomitant paralytic strabismus.


Cyclic esotropia--a case history.

Cyclic esotropia--a case history.


Ophthalmologic and orthoptic examination of 1,200 children up to age 2.


Fresnel prism membrane in acquired extraocular muscle palsy.


Editorial: The dermatology foundation.

Editorial: The dermatology foundation.


Report on ultraviolet light sources. Report by the task force on photobiology of the national program for dermatology.


Acquired immunity to dermatophytes.

Acquired immunity to dermatophytes.


Atrophie blanche. A sign of dermal blood occlusion.


Topically active corticosteroids. A quantitative evaluation of McKenzie's skin-blanching test.


Diet plan for patients with salicylate-induced urticaria.


Ultrastructural study of a case of angiolymphoid hyperplasia with eosinophilia.


Unilateral nevus of Ota with sensorineural deafness.


Flucytosine. An effective oral treatment for chromomycosis.


Letter: Toxic epidermal necrolysis caused by "gin and tonic".


Infantile syphilis accompanied by anticomplementary effect of Cryoglobulin. Report of a case.


Letter: Severe contact dermatitis from poinsettia.


Letter: 2,4-Dinitrochlorobenzene therapy for plantar warts.


Letter: Punctate keratoderma.

Letter: Punctate keratoderma.


Letter: Idiopathic pigmentation of the upper back.


Letter: Paired hairs.

Letter: Paired hairs.


Letter: Transient acantholytic dermatosis.


Letter: Grenz ray shielding.

Letter: Grenz ray shielding.


Letter: Subcorneal pustular dermatosis controlled by vitamin E.


Letter: Fistula of the dorsum of the nose.


Drugs for bed-wetting.

Drugs for bed-wetting.


Enuresis and spontaneous cure rate. Study of 1129 enuretis.


Ascorbic acid in fetal human brain.

Ascorbic acid in fetal human brain.


Effect of moderate dosage of chlorpropamide in pregnancy on fetal outcome.


Renal loss of folate in the newborn infant.


Poisoning with delayed-release tablets. Treatment of Debendox poisoning with purgation and dialysis.


Primary somatomedin deficiency. Case report.


Hypertension in Nigerian children.

Hypertension in Nigerian children.


Premedication for jejunal biopsy in childhood using intravenous diazepam and metoclopramide.


Mixed pneumococcal and tuberculous meningitis.


Diazepam for fenfluramine intoxication.

Diazepam for fenfluramine intoxication.


The speech pathologist and the reading process.


Guidelines for audiometric symbols.

Guidelines for audiometric symbols.


Error rates in cervical cytological screening tests. In an attempt to clear up the confusion evident in the literature concerning some aspects of cervical cytological screening tests, the principle is stated upon which the data acquired in a series of tests should be tabulated. The table is used to define several rates or probabilities, most of which express the rates at which errors occur. Certain rates are distinguished as of basic importance, others playing only a secondary role. The inter-relations of the rates are displayed as equations and reference is made to a set of conversion tables constructed from the equations. As an illustration, the data from a particular published paper is treated in detail, showing how the various rates can be calculated or estimated, and, in passing, also demonstrating their high degree of uncertainty.


Further studies on induction of stomach cancer in hamsters by N-methyl-N'-nitro-N-nitrosoguanidine. Oral administration of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) to hamsters at a concentration of 50-83 μg/ml in the drinking water resulted in a high incidence of tumours in the glandular stomach. Short-term administration of MNNG for 4-6 months resulted in more adenocarcinomata in the glandular stomach than long-term administration for 7-8 months. One case of metastasis of an adenocarcinoma of the glandular stomach to the liver and 2 cases of metastasis to the regional lymph nodes were found. Spindle cell sarcomata in the glandular stomach and adenocarcinomata in the duodenum were also often produced.Oral administration of MNNG at the very high concentration of 500-2000 μg/ml induced a hepatic cell carcinoma, intrahepatic bile duct carcinomata, bile duct cystadenomata and cystic dilatation, and a haemangioma in the liver but no tumour in the glandular stomach.Sequential morphological studies on the glandular stomach of hamsters receiving 50 μg/ml of MNNG in the drinking water showed 3 stages of change of the mucosa. The mucosa became atrophic and eroded in the first 16 weeks. Irregular atypical glands developed at the margins of erosions and proliferation of spindle cells in the submucosa were found after 18 weeks. Spindle cell sarcomata developed in animals after 20 weeks. Adenocarcinomata developed between 25 and 32 weeks.


A method of comparing differences in tumour growth rates applied to a study of the increasing growth capacity of mouse carcinomata. A method of comparing differences in growth rates of tumours in small groups of animals is described. A common slope can be fitted to the growth curves of a given tumour in a group of isogeneic animals. Differences between growth potentials can be demonstrated by comparing the common slope for a given tumour against that of another tumour (or the same tumour at a later stage of development).A highly significant difference is shown between the growth potential of an A-strain mammary carcinoma after 7 or after 28 days' growth in isogeneic animals. Since this increase in autonomy is reflected in the tumour's subsequent growth rate in secondary hosts, it is suggested that it involves adaptation of the tumour rather than progressive immunodepression of the primary host.


Rural preponderance of testicular neoplasms.


Microinvasive carcinoma of the hard palate.


Development of specific cell-dependent antibody during growth of a syngeneic rat sarcoma. A micro-cytotoxicity assay was adapted for the detection of cell-dependent antibodies (CDA). Using normal rat spleens as the source of effector cells such CDA activity was readily demonstrable in allo-immune sera tested on cultured sarcoma cells. The same technique was then used to examine for tumour specific antibodies in the sera of Hooded rats bearing a "non-immunogenic" syngeneic metastasizing sarcoma. During the early stages of tumour growth, at Days 7 and 14, tumour specific CDA cytotoxicity was detectable at high titres. By Day 21, however, this activity had completely disappeared from the serum. This cell-dependent cytotoxicity was tumour specific in that it did not kill cells from an unrelated syngeneic sarcoma, and the activity was probably confined to immunoglobulin G as detected by molecular weight separation techniques. Following tumour amputation at Day 21, this type of specific antibody activity rapidly re-appeared in the serum. The presence of tumour specific CDA showed an inverse correlation with the presence of specific inhibitors of cell-mediated immunity in the same sera. At no stage in tumour growth could complement-dependent cytotoxicity be detected in tumour bearing rat sera.It is concluded that cell-dependent cytotoxic activity is not associated with conventional complement dependence, that this CDA type of assay is exquisitely sensitive and is suitable for the detection of anti-TSTA antibodies in tumour bearing rats.The possible significance of CDA activity in syngeneic tumour immunity is discussed briefly. The results suggest that the role of humoral immune mechanisms in host resistance to tumour growth needs re-appraisal.


The incidence of malignant tumours in patients with respiratory sarcoidosis. During the period 1962-71 a total of 2544 patients with respiratory sarcoidosis were reported to the Danish Institute of Clinical Epidemiology. Among them 48 patients developed a malignant tumour, the follow-up period ending on 31 December 1971. Only 33·8 cases of cancer were expected if sarcoidosis patients had had the same rates as the general population; the difference between the expected and observed number is statistically significant (0·02 > P > 0·01). Malignant lymphomata occurred 11 times and lung cancer 3 times more frequently than expected. For all other forms of cancer taken together, there was no significant difference between the expected and the observed number of cases.The increased cancer incidence may result from immunological deficiencies in patients with sarcoidosis.


Anti-tumour effect in vitro of lymphocytes and macrophages from mice treated with Corynebacterium parvum. Cells from the spleen, lymph node, peripheral blood and peritoneal exudate of mice treated with C. parvum were tested for their ability to inhibit tumour growth in vitro. The peritoneal exudate cells from C. parvum treated mice were extremely effective in inhibiting tumour growth whereas the spleen and peripheral blood cells were only moderately so. In contrast, the lymph node cells caused only a modest inhibition of tumour growth at a very high effector to target cell ratio. Spleen cells from normal mice also exerted a moderate anti-tumour effect.


Breast volumes in cancer of the breast.

Breast volumes in cancer of the breast. A method has been devised to calculate breast volumes from mammograms. This has been applied to mammograms from 42 women with breast cancer and 42 age-matched normal controls. No difference in breast volumes was noted.


A bone dysplasia with deafness.

A bone dysplasia with deafness.


Per-operative cerebral angiography.

Per-operative cerebral angiography.


The choroidal point and the copular point.


Meglumine diatrizoate v. iothalamate: comparison of seizure-inducing potential.


Monomer-dimer contrast media in the renal circulation: experimental angiography.


A useful sign in the diagnosis of bladder tumours on intravenous urograms.


Excess late subcutaneous fibrosis after irradiation of pig skin, consequent upon the application of the NSD formula.


Universal radiation portal marker.

Universal radiation portal marker.


A self-masking film illuminator.

A self-masking film illuminator.


Pseudo-abscess: anomalous colon simulating peri-renal infection.


Letter: Radiology now--the "10-day rule".


Letter: Special radiation units and-or SI units?


Letter: SI units for radiation measurements.


Letter: The lymphocyte response to phytohaemagglutinin after in vitro irradiation.


Letter: "High-dose" metronidazole: a preliminary pharmacological study prior to its investigational use in clinical radiotherapy trials.


Letter: The use of tolazoline hydrochloride (Priscoline) in the treatment of complications at arteriography.


Letter: Skin dose from handling unshielded syringes.


Letter: Dangers of re-sterilized disposable syringes in urography.


Letter: "Unity of radiology".

Letter: "Unity of radiology".


Mass phenotyping of isoniazid inactivators by automated determination of acetylisoniazid in urine.


Improved assay for spinal fluid glutamine, and values for children with Reye's syndrome.


Comparison of five methods for determination of 2,3-diphosphoglycerate in blood.


Immunoglobulin concentrations in sera of normal children: quantitation against an international reference preparation.


Quality-control system for blood pH and gas measurements, with use of a tonometered bicarbonate-chloride solution and duplicate samples of whole blood.


Screening cord bloods for detection of sickle cell disease in Jamaica.


Standard reference materials 933 and 934: the National Bureau of Standards' precision thermometers for the clinical laboratory.


Contamination of distilled water, HCl, and NH4OH with amino acids, proteins, and bacteria.


Rapid ultraviolet procedure for measuring doxepin and some doxepin metabolites in human urine.


Use of co-current dialysis in an improved estimation of free thyroxine in serum.


Quality-control considerations with the Du Pont Automatic Clinical Analyzer (ACA).


Radioimmunoassay of insulin, with use of an immunosorbent.


Clinical studies of alcoholic ketoacidosis.


Effect of moderate alcohol intake on control of diabetes.


Insulin action on the cultured human fibroblast. Glucose uptake, protein synthesis, RNA synthesis.


Insulin and glucose responses to identical oral glucose tolerance tests performed forty-eight hours apart.


Relationship between insulin response during the intravenous glucose tolerance test, rate of fractional glucose removal and the degree of insulin resistance in normal adults.


Purification and immunologic identification of aldose reductases.


Evaluation of some commonly used semiquantitative methods for urinary glucose and ketone determinations.


Translation of histone messenger RNA by homologous cell-free systems from synchronized HeLa cells.


Analysis of the reactivity of SH-reagents with the mitochondrial phosphate carrier.


Luteinizing hormone. 2. Relative reactivities of tyrosyl residues of the porcine hormone towards iodination.


(4-Hydroxy-3-nitro)benzylated polystyrene. An improved polymeric nitrophenol derivative for peptide synthesis.


Studies by X-ray small-angle scattering of the quaternary structure in solution of halves and tenths of Helix pomatia haemocyanin and of Sepia officinalis haemocyanin.


The separation of neurotoxin from the venom of Naja melanoleuca and the primary sequence determination.


The molecular weight and subunit structure of human erythrocyte 6-phosphogluconate dehydrogenase.


A kinetic study of the deacylation of alpha-benzamido-trans-cinnamoyl-chymotrypsin. Evidence for the intervention of non-enzymic species.


Ribosome precursor particles in the nucleolus of rat liver. Easily extractable nucleolar 60-S ribonucleoprotein particles and their relation to cytoplasmic large ribosomal subunits.


A dithioamino acid in kinetic studies on trypsin catalysis. The tryptic S-alkyl cleavage of ethyl 6-ammoniumdithiohexanoate p-toluene sulfonate.


Luteinizing hormone. I. Circular dichroism and spectrophotometric titration of porcine and bovine hormones and of their alpha and beta subunits.


Tritium as a tracer for reducing equivalents in isolated liver cells.


Identification of serine and histidine as essential amino-acid residues in the coagulant enzyme ancrod.


A linear steady-state treatment of enzymatic chains. General properties, control and effector strength.


A linear steady-state treatment of enzymatic chains. Critique of the crossover theorem and a general procedure to identify interaction sites with an effector.


Evidence for a role of the microtubular system in the secretion of newly synthesized albumin and other proteins by the liver. Livers of normal mice were prefused in situ and the secretion of newly synthesized (i.e. labeled) proteins into the perfusate were measured. In control livers, the secretion of newly synthesized proteins was found to be linear with time. In marked contrast, when livers were perfused with vinblastine, vincristine, or colchicine, drugs known to interfere with the hepatic microtubular system, the release of newly synthesized proteins was either strongly inhibited or completely suppressed although total hepatic protein synthesis (estimated by the incorporation of labeled amino acids into hepatic plus perfusate proteins) remained unaltered. Chromatographic separation of the various secreted proteins showed that the release of albumin, globulins, and small polypeptides was decreased to a similar extent by vincristine or colchicine. In the particular case of albumin, it was further observed that total (i.e. liver plus perfusate) labeled amino acid incorporation into albumin was not altered by either vincristine or colchicine, whereas the incorporation of these amino acids into liver albumin was markedly increased but incorporation into perfusate albumin was decreased, suggesting that the translocation of this particular protein from the liver to the perfusate had been affected by the presence of these drugs. It is proposed that the functional integrity of microtubules is necessary for the intracellular movement and eventual release of albumin and other proteins by the liver, and suggested that microtubules might possibly be a site of regulation of hepatic protein secretion.


Evidence for different gestation-dependent effects of cortisol on cultured fetal lung cells. The effect of cortisol (5.5 muM) on primary monolayer cultures of trypsin-dispersed lung cells from rabbit fetuses of 20-28 days gestation was monitored with respect to (a) cellular growth as determined by DNA content after 72 h, at which time all cultures were in the exponential phase of growth, and (b) cellular maturation as reflected by the incorporation of [(14)C]-palmitate into saturated lecithin and its release into the culture medium. Cortisol significantly increased growth in cultures prepared from 20 day (control: 59.8+/-8.9 nmol DNA/flask; cortisol: 118.7+/-15.7, P < 0.001) and 22 day (control: 69.2+/-17.2; cortisol: 106.7+/-13.3, P < 0.001) fetuses but had no effect on the growth of cells from 24 or 26 day fetuses. At 28 days, the effect was reversed, cortisol reducing growth by a factor of two (control: 42.0+/-8.5; cortisol: 19.3+/-4.0, P < 0.001). Incorporation of palmitate into lecithin was expressed as picomoles incorporated per micromole DNA per flask, thus correcting for differences in the number of cells. Cortisol had no effect on palmitate incorporation until day 26, at which time it caused a slight increase (control: 51.2+/-5.5: cortisol: 72.8+/-16.2, P < 0.01) which became very striking by day 28 (control: 19.7+/-3.1; cortisol; 286.8+/-47.0, P < 0.001). The proportion of recovered radiolabeled lecithin that was disaturated rose with gestational age from 72% at 20 days to 98% at 28 days. Saturated lecithin made up over 90% at the two gestational ages (26 and 28 days) where cortisol increased palmitate incorporation. In contrast, cortisol had no effect on the incorporation of palmitate into sphingomyelin at any of the gestational ages studied.The results suggest that cortisol may increase fetal pulmonary cellular growth in early gestation while enhancing maturation and slowing growth as term approaches.


Storage iron kinetics. VII. A biologic model for reticuloendothelial iron transport. The processing of erythrocyte iron by the reticuloendothelial cell has been characterized by kinetic measurements of blood radioactivity made after the intravenous injection of heat-damaged erythrocytes labeled with (59)Fe and of transferrin-bound (55)Fe. The early reticuloendothelial release of iron, a matter of hours, was calculated from the plasma turnover rate of (55)Fe and the plasma reappearance of (59)Fe. Late release was calculated from the ratio of the cumulative incorporation of both tracers into the circulating red cell mass over a period of 2 wk. There was an initial processing period within the reticuloendothelial cell, after which radioiron either rapidly returned to circulation (t(1/2) 34 min) or was transferred to a slowly exchanging pool of storage iron within the reticuloendothelial cell (t(1/2) release to plasma of 7 days). These pathways were of equal magnitude in the normal dog. Reticuloendothelial release of iron was largely independent of the pre-existing plasma iron level or transferrin saturation. Diurnal fluctuations in the plasma iron level were shown to be the result of a variable partitioning of iron between the early and late release phases. Acute inflammation resulted in a prompt and marked increase in the fraction of iron stored (late phase), whereas depletion of iron stores resulted in a marked increase in early release.


The presence of a natriuretic factor in urine of patients with chronic uremia. The absence of the factor in nephrotic uremic patients. A gel filtration fraction of serum from chronically uremic patients has been shown previously to produce natriuresis in the rat. In the present studies, the same fraction from urine of uremic patients and normal subjects was studied for its natriuretic activity. Urine samples were obtained from 17 chronically uremic patients (mean glomerular filtration rate [GFR], 8.7 ml/min; mean fractional sodium excretion [FE(Na)], 5.7%), and 14 normal subjects. The fraction from the uremic patients produced a significant increase in absolute sodium excretion (U(Na)V) and FE(Na); the fraction from normal subjects had no statistically significant effect on either U(Na)V or FE(Na); and the difference between the response to the uremic vs. normal fractions was highly significant for both parameters of sodium excretion. When a more concentrated urine fraction from uremic patients was administered, a striking natriuresis was observed with values for FE(Na) rising to levels as high as 12%. Studies also were performed on eight patients with far advanced chronic renal insufficiency and the nephrotic syndrome. The serum fraction was studied in each of these patients and the urine fraction in three. For the group, U(Na)V in the assay rats decreased by 0.87 mueq/min and FE(Na) decreased by 1.35% after infusion of the serum fraction. These results differ significantly from those of patients with chronic uremia without the nephrotic syndrome. The data are consistent with the view that the increased activity of the natriuretic factor in the serum of chronically uremic patients is not due to failure of excretion; rather it relates either to an increased rate of production and/or a decreased rate of degradation. The data also show that the inhibitor is detectable when FE(Na) is increased, but not when uremia is associated with a sodium-retaining state.


Studies of the mechanism of oliguria in a model of unilateral acute renal failure. To further evaluate the mechanism of the oliguria of acute renal failure, a model was utilized in which intense and prolonged vasoconstriction produced the unilateral cessation of urine flow. The radioactive microsphere method was used to measure total and regional blood flow before and after the intrarenal infusion of norepinephrine, 0.75 mug/kg/min, for 2 h in the dog. In the control kidney, renal blood flow increased 32% 48 h after norepinephrine in association with a fall in the fractional distribution of flow to the outer cortex. In the experimental kidney, total renal blood flow fell from 190 ml/min before norepinephrine to 116 ml/min at 48 h (P < 0.025) with a uniform reduction in cortical blood flow. After the administration of 10% body wt Ringer's solution, there was a marked redistribution of flow to inner cortical nephrons in both the control and experimental kidney. In addition, there was a marked increase in total blood flow in both kidneys. On the experimental side, flow rose to 235 ml/min, a value greater than in either the control period (P < 0.05) or at 48 h after norepinephrine (P < 0.001). However, in spite of this marked increase in blood flow, there was essentially no urine flow from the experimental kidney. In separate studies, the animals were prepared for micropuncture. In all studies, the surface tubules were collapsed, and there was no evidence of tubular obstruction or leakage of filtrate. Over 99% of the 15-muM spheres were extracted in one pass through the experimental kidney. An analysis of the forces affecting filtration suggested that an alteration in the ultrafiltration coefficient may be responsible, at least in part, for the anuria in this model. In this regard, transmission and scanning electron microscopy revealed a marked abnormality in the epithelial structure of the glomerulus. It is suggested that a decrease in glomerular capillary permeability may be present in this model of acute renal failure.


Coronary hemodynamics and regional myocardial metabolism in experimental aortic insufficiency. Acute aortic valvular insufficiency was induced in open chest dogs by employing a special intravascular cannula, or by rupturing an aortic valve leaflet. Phasic and mean coronary flow were assessed in some animals, while in others data were obtained on arterial and coronary sinus blood lactate, pyruvate, P(O2), P(CO2), and pH, and on myocardial tissue lactate, pyruvate, and water content in the outer and inner halves of the free wall of the left ventricle. Results showed that in acute aortic insufficiency diastolic coronary flow decreased as a function of aortic diastolic pressure, but systolic coronary flow increased in such proportion that mean coronary flow did not decrease. With moderate reductions in aortic diastolic pressure due to aortic insufficiency, myocardial blood flow was judged to be nutritionally adequate in both the outer and inner regions of the left ventricle. With more severe reductions in aortic diastolic pressure, the inner region exihibited biochemical signs of anaerobic metabolism. The presence of these metabolic changes could be correlated with either of two previously described pressure indexes. These findings suggest that the reduced coronary perfusion pressure and the intramyocardial tissue pressure gradient can be compensated for by autoregulation in some cases of aortic insufficiency, but in others such compensation may be incomplete, in which case oxygen delivery to the subendocardium will be inadequate to meet local tissue oxygen needs.


Metabolic studies of the third component of complement and the glycine-rich beta glycoprotein in patients with hypocomplementemia. Metabolic studies using radioiodine-labeled third component of complement (C3) and the glycine-rich beta glycoprotein (GBG), a major component of the C3b-feedback pathway, were undertaken in normal subjects, in 22 patients with evidence of complement activation, and in 11 patients with various renal diseases without evidence of complement activation. In seven normal subjects GBG was found to be a rapidly metabolized protein with catabolic rates ranging from 1.7% to 2.2% of the plasma pool/h, synthesis rates from 0.14 to 0.21 mg/kg per h. and extravascular/intravascular distribution ratios from 0.81 to 1.31. In patients with reduced plasma C3, both increased C3 fractional catabolic rates and reduced C3 synthesis rates were observed, and in some patients there was evidence of increased extravascular distribution of the protein. GBG catabolism was usually increased when there was evidence of C3 activation, presumably reflecting activation of the C3b-feedback; but GBG turnover was normal or only slightly accelerated in some patients with accelerated C3 catabolism and profound hypocomplementemia, suggesting that reduced C3 synthesis had limited activation of the C3b-feedback.