Human liposarcomas: tissue cultures containing foci of transformed cells with viral particles. Viral particles occurring in foci of human liposarcoma cells in tissue culture are morphologically similar to the sarcoma viruses of the avian and murine species. Antibodies in the serum of the patient from whom the culture was originated reacted with cytoplasmic antigens in the original liposarcoma and in the cultured liposarcoma cells.


Impact of Medicare upon a private practice of ophthalmology.


Part I. Sequential hemodynamic changes in an experimental hemorrhagic shock preparation designed to simulate clinical shock.


Silver nitrate resistant E. coli: report of case.


Effect of endogenous acidification of the duodenum on the Heidenhain pouch pepsin response to histamine.


Recombination between Escherichia coli and rough forms of Shigella flexneri.


Tye use of the immunofluorescence adsorption test (IFAT) for titrating tetanus anatoxins.


Blood groups in rabbits detected by means of posttransplantation hemagglutinins.


The influence of the autonomic nervous system on phagocytosis. V. Changes caused by chronic administration of methylatropine nitrate.


The influence of the autonomic nervous system on phagocytosis. VI. Changes caused by chronic administration of dihydroergotamine.


Fluorescent protein tracing in multiple sclerosis brain tissue.


Parkinson's syndrome.

Parkinson's syndrome.


The presence of N-formyl-methionyl-tRNA in HeLa cell mitochondria.


DNA-dependent RNA polymerase from phage T4 infected E. coli: an enzyme missing a factor required for transcription of T4 DNA.


Acid labile sugar in pepsinogen.

Acid labile sugar in pepsinogen.


Adenyl cyclase of Escherichia coli.

Adenyl cyclase of Escherichia coli.


A soluble enzyme from Escherichia coli which catalyzes the transfer of leucine and phenylalanine from tRNA to acceptor proteins.


A particle bound intermediate in the biosynthesis of plant cell wall polysaccharides.


Hyperbaric oxygenation.

Hyperbaric oxygenation.


Some features of medical education in Great Britain during the first half of the nineteenth century.


A double-blind trial of disodium cromoglycate in bronchial asthma wh assessment by four observers.


Human pharmacological and clinical studies on salbutamol: a specific beta-adrenergic bronchodilator.


On medical nomenclature.

On medical nomenclature.


Infectious mononucleosis, lymphoblastic leukemia, and the E. B. virus.


Acute rejection following cardiac transplantation. Phonocardiographic and ultrasound observations.


Heterotransplantability of human cell lines derived from leukemia and lymphomas into immunologically tolerant rats.


Streamlined billing for Medicare outpatients.


Listing of hospitals; United States.

Listing of hospitals; United States.


A simple assay of dopamine beta-hydroxylase activity in the homogenate of the adrenal medulla.


Listing of hospitals in areas associated with the United States.


U.S. Government Hospitals outside the United States.


Canadian hospitals.

Canadian hospitals.


AHA membership.

AHA membership.


Health organizations: international, national and regional.


National health agencies: United States, Canada. Selected federal agencies by department.


State and provincial health organizations and agencies.


Professional schools.

Professional schools.


Search for an adenovirus etiology of human oral and pharyngeal tumors.


Transformation of foetal human keukocytes in vitro by filtrates of a human leukaemic cell line containing herpes-like virus.


The medical projectors, 1640-1720.

The medical projectors, 1640-1720.


Guy de Chauliac's theory of the humors.

Guy de Chauliac's theory of the humors.


Medical genealogy.

Medical genealogy.


Barnacle larvae in the nineteenth century. A case study in taxonomic theory.


The contributions of J.F. Meckel, the Younger, to the science of teratology.


Death too many for the doctor. Drawing by S. Collings engraved by F. Jukes published by R. Pollard (14 February 1803).


The traditional taenicides of Ethiopia.

The traditional taenicides of Ethiopia.


Notes and events.

Notes and events.


Changes in the growth curves of E. coli following the incorporation of [6-3H] thymidine and [G-3H] uridine.


Nicolò Massa, d. 1569.

Nicolò Massa, d. 1569.


Delayed hypersensitivity and inhibition of macrophage spreading: in vivo and in vitro studies of tuberculin and streptococcal hypersensitivities in guinea pigs.


Genetic factors in leucocyte responses to endotoxin: further studies in mice.


Tolerance induction with thymus grafts in neonatally thymectomized mice.


Induction of abnormal cell division in Euglena gracilis by glutamic diethyl ester.


Structure and respiration of sporulating Eimeria stiedae and E. tenella oocysts.


Changes in urinary output and free water clearance in patients with acute circulatory failure (shock).


Autotransplantatio of a slitary functioning kidney for renovascular hypertension.


Post-intubation subglottic granulation tissue: review of the problem and evaluation of radiotherapy.


Laryngotracheoesophageal cleft. Report of a case and review of the literature.


Movement of fibroblasts in a spatially confined system.


Induction of immunological tolerance by porcine liver allografts.


Infrared spectrum of an anticodon fragment of a transfer RNA.


Glucose intolerance in cystic fibrosis.

Glucose intolerance in cystic fibrosis.


Development of endocrine function in the human placenta and fetus (first of two parts).


Rhinoplasty.

Rhinoplasty.


Convincing the sceptics.

Convincing the sceptics.


[On-going national collaborative studies in Czechoslovakia].


The history of the University of Pittsburgh School of Medicine.


Fine structure analysis of a chromosome segment in Drosophila melanogaster: analysis of ethyl methanesulphonate-induced lethals.


On-going national collaborative studies in Japan.


"Excision repair" and dose-modification: questions raised by radiobiological experiments with acriflavine.


Lethal and mutagenic effects of irradiated medium on Escherichia coli.


On-going national collaborative studies in France.


The mutability toward ultraviolet light of recombination-deficient strains of Escherichia coli.


Hematological and virological studies of epizootic hemorrhagic disease of deer.


Chemical mutagenesis in E. coli B-R; the influence of repair systems for UV damage.


Homograft skin: a versatile biologic dressing.


Mass detection of significant bacteriuria. An improved triphenyltetrazolium chloride (TTC) technique.


Our role in the generation, modification, and termination of life.


Regeneration, tumor, dietary, and L-asparaginase effects on asparagine biosynthesis in rat liver.


The kinetics of the organ-specific metastasis of a transplantable reticuloendothelial tumor.


Sickle cell trait and pregnancy.

Sickle cell trait and pregnancy.


Association of pelvic inflammatory disease with the intrauterine device.


Pathophysiology of the hemoglobinopathies.


Use of anticoagulants in suspected pelvic infection.


Hemoglobinopathies: genetics and implications for studies of human reproduction.


Attempt at inducing male-specific antigen in female skin.


Tolerance manifestations among inbred rat strains defined in the Rt H-1 antigens. I. Runt syndrome incidence and skin graft tolerance.


Comments on untoward reactions of the horse to injection of antigenic substances.


Mixed equine bacterins.

Mixed equine bacterins.


Cholestyramine treatment of diarrhea associated with ileal resection.


Contact dermatitis due to 1-chloroacetophenone and chemical mace.


Professional advisory committees and Medicare.


Lactulose in the treatment of chronic portal-systemic encephalopathy. A double-blind clinical trial.


Total patient care. The team approach to transplanation.


Gene action and behavior: an evaluation of major gene pleiotropism.


Retrograde effects of electroconvulsive shock on learned responses.


Ultrastructural aspects of infections in the central nervous system.


A review of water and electrolyte shifts in the central nervous system during infection.


Ribonucleic acid virus replication.

Ribonucleic acid virus replication.


The microcirculatory events within full-thickness skin allografts (homografts) in mice.


Arterial suture techniques and grafts: past, present, and future.


Review of pancreatic disease.

Review of pancreatic disease.


Allografting split-thickness skin donor sites.


Shouldice repair for inguinal hernia.

Shouldice repair for inguinal hernia.


Esophageal tamponage in the treatment of massive bleeding from esophageal varices with special reference to volume and pressure in the balloons.


Rosalie Shohl, M.D. 1927-1969.

Rosalie Shohl, M.D. 1927-1969.


Appendicitis associated with Shigella sonnei infection.


Increase in myocardial collateral capacity following drug-induced coronary vasodilatation. A preliminary report.


Chaotic atrial mechanism.

Chaotic atrial mechanism.


Effects of acetazolamide on cerebral blood flow of dogs during hyperbaric oxygenation.


Respiratory and cardiac effects on venous return.


Rostan on asthma in the aged (cardiac asthma), 1818.


Coronary and precoronary care.

Coronary and precoronary care.


The effects of Francisella tularensis infection on iron metabolism in man.


Increased rate of clearance of drugs from the circulation of alcoholics.


Upper extremity blood flow in patients with chronic obstructuve airways disease.


Canine heart allograft rejection and spontaneous in vitro transformation of peripheral blood lymphocytes.


Bactericidal activity of the ampicillin-kanamycin combination against Escherichia coli, Enterobacter-Klebsiella and Proteus.


Theophylline and potassium iodide in the treatment of chronic asthma.


Membrane potential and muscle function.

Membrane potential and muscle function.


Technetium-99m in clinical nuclear medicine.


Neurosurgical control of pain.

Neurosurgical control of pain.


Chlamydial infection in Reiter's syndrome.


Renal tubular acidosis.

Renal tubular acidosis.


Ureteral motility.

Ureteral motility.


The defective red blood cell in hereditary spherocytosis.


The psychopharmacology of hallucinogenic agents.


Platelet lifespan.

Platelet lifespan.


The effects of physiological concentrations of Mg++ and K+ on the temperature-absorbancy curve of DNA.


The case of Mary, Queen of Scots. Some further medical notes.


The retina and optic nerve.

The retina and optic nerve.


William Lemuel Benedict, MD. 1885-1969.

William Lemuel Benedict, MD. 1885-1969.


Concomitance of bacteria on various areas of the eye.


Rapid equilibrium isopycnic CsC1 gradients.


The isolation and the characterization of DNA region bound to Escherichia coli RNA polymerase.


Intermediate stage in the association and dissociation of Escherichia coli ribosomes and the combining properties of their subunits.


Preservation of functional ribosomes by freeze-drying.


Cornelius Gysbert Dyke: pioneer neuroradiologist. Condensed from The First Cornelius G. Dyke Memorial Lecture.


A study of gastric secretion induced by close arterial infusions of histamine in the dog.


Dr. J. Earle Hiltz. An appreciation.

Dr. J. Earle Hiltz. An appreciation.


Further studies on the effect of various drugs on the uptake of tyramine-3H and formation of octopamine-3H in the rat heart.


Stereotactic coordinates for the human dentate nucleus.


Growth and physical performance of American Negro and white children. A comparative survey of differences in body size, proportions and composition, skeletal maturation, and various motor performances.


The healing of cartilage defects.

The healing of cartilage defects.


Regulations, standards, and guides pertaining to medical and dental radiation protection--an annotated bibliography.


The results of surgery for polydactyly of the thumb. A review.


Purification and properties of DNA-dependent RNA-polymerase from Bacillus stearothermophilus.


The work of Alfred Worcester.

The work of Alfred Worcester.


Exoerythrocytic stages of avian and reptilian malarial parasites.


The existential approach to religion.

The existential approach to religion.


Morality, guilt, and forgiveness in psychotherapy.


Primitive religious rites and behavior: clinical considerations.


Quantity and distribution of indigenous flora in a hospital environment.


Technical information resources in the air pollution field.


J. F. Volker named president of U of Alabama in Birmingham.


Physical separation of hemopoietic stem cells differing in their capacity for self-renewal. Bone marrow cells in suspension were separated into a number of fractions on the basis of cell size by sedimentation at unit gravity through gradients of fetal calf serum. The colony forming units (CFU) from the various fractions were tested for their self-renewal capacity using a double transplantation technique. The results indicate that the CFU in the fractions containing slowly sedimenting cells have an increased capacity for self-renewal in comparison with CFU in fractions containing rapidly sedimenting cells. In addition, a culture method was used to select populations containing CFU with increased self-renewal capacity, and these CFU were shown to sediment slowly in comparison with CFU of lower self-renewal capacity obtained from control cultures. It may be concluded that at least part of the heterogeneity observed in the CFU content of individual spleen colonies arises from the composition of the initial cell suspension, probably from intrinsic differences between the stem cells themselves.


Harvey Cushing and the humanities in medicine.


The New England Journal of Medicine, 1812-1968.


Compulsory health insurance in California, 1915-18.


On the provenance of some early medical and biological books.


Meteorology and medicine in colonial America: beginnings of the experimental approach.


Proposal to fix Medicare fees of physicians for 15 months.


Operation for stones in the head. An engraving by Nicolaes Weydmans.


The exoerythrocytic stages of Plasmodium falciparum in Aotus trivirgatus.


Stochastic models for tracer experiments in the circulation. II. Serial random walks.


Renal homotransplantation.

Renal homotransplantation.


Great men.

Great men.


The use of a peptidase inhibitor in the treatment of acute pancreatitis.


Medical philately of Australia.

Medical philately of Australia.


David William Johnson.

David William Johnson.


John Robert Adam.

John Robert Adam.


William Keverall McIntyre.

William Keverall McIntyre.


Separation, identification, and estimation of prostaglandins. The procedures which may be and are being used to provide a basis for the analysis of submicrogram quantitities of prostaglandins are surveyed.  Discussion is focused on the following: 1) sources of standards; 2) properties (effect of different pH values, effect of blood, metabolism, solubility); 3) extraction; 4) detection; 5) estimation (ultraviolet, optical rotatory dispersion, densitometry, radioimmunoassay, enzymatic assay, isotopic methods, bioassay); 6) separation of prostaglandins (separation of PGE, PGF, and PGA with PGB compounds, separation of PGA and PGB compounds, and separation of individual prostaglandins); and 6) structural identification.  Methods of prostaglandin analysis, with the required sensitivity for application to individual tissue and fluid specimens, are still in the developmental state.  Although prostaglandins may be ubiquitous throughout the animal kingdom, no systematic study of their distribution has been made to date.  Recent work has shown that PGE1 has a potent effect on the formation of 3',5' cyclic adenosine monophosphate (cyclic AMP) which is widely believed to be an intracellular intermediate in hormone action.


Practical aspects of liquid scintillation counting.


Surgery for hearing loss.

Surgery for hearing loss.


Inherited aminoacidopathies demonstrating vitamin dependency.


Neuropsychopharmacology and the affective disorders.  (Third of three parts)


John Upham Williams.

John Upham Williams.


Estrogen assays in the clinical practice of gynecology and obstetrics.


Flexibility.

Flexibility.


Paul Budd Magnuson, M.D. 1884-1968.

Paul Budd Magnuson, M.D. 1884-1968.


Ignaz Philip Semmelweis. 1818-1968.

Ignaz Philip Semmelweis. 1818-1968.


The effects of air pollution on health.

The effects of air pollution on health.


Mirror-image stimulation.

Mirror-image stimulation.


Hypnotic and non-hypnotic revivification with special reference to Jack London's "Martin Eden".


Colony-forming cells in irradiated, partially shielded, endotoxin-treated mice.


Chemical protection against single-strand breaks in DNA of gamma-irradiated E. coli.


Crystalline L-asparaginase from escherichia coli B. L-Asparaginase has been crystallized from a partially purified extract of Escherichia coli B. The crystalline enzyme is homogeneous, as judged by analytical polyacrylamidegel electrophoresis and sedimentation behavior. This enzyme preparation is active in preventing lymphoma in mice and also has low glutaminase activity.


The role of the complement system in rheumatoid inflammation.


Wisdom in the pursuit of excellence--a history of the Pacific Coast Oto-Ophthalmological Society.


Renal transplantation in the inbred rat. 8. Quantitation of cell proliferation during unmodified rejection.


Exceptions to the genetic rules of transplantation.


Transplantation immunosuppression by local infusion of allografts of canine lung.


Ultrastructural study on nuclear-cytoplasmic relationships in oocytes of the African lungfish. Protopterus aethiopicus. I. Nucleolo-cytoplasmic pathways.


Fine structure of the wolffian duct and cytodifferentiation of the epididymis in fetal rats.


An electron microscope study of the perfusion-fixed spleen. II. Nurse cells and erythrophagocytosis.


Nobecutane in the exposure treatment of burn wounds.


Treatment of hemochromatosis.

Treatment of hemochromatosis.


Classification of reactive hypoglycemia.


A second clinical trial to compare two methods for preoperative preparation of the large bowel.


Localized immunosuppression for pulmonary transplants.


Carcinoma of the breast--a clinical trial.


A controlled trial of venous thrombosis prophylaxis with dextran 70.


Different aspects of antilymphocyte globulin (ALG) treatment in man.


A controlled clinical trial to compare transethidal hypophysectomy with yttrium implant of the pituitary in the treatment of advanced breast cancer.


Ultrasonic blood-flow studies in renal transplantation.


Physiological responses of the preserved autotransplanted canine kidney.


Lymphocyte depletion and renal transplantation in the calf.


Transplantation of second cadaver donor kidneys.


Renal preservation and immunological rejection reaction of stored kidneys.


Release of prostaglandins caused by distension of the lungs.


Comparison of pyloroplasty and gastrojejunostomy.


Current status of the management of patients with endocrine-sensitive tumors. II. Carcinoma of the prostate, endometrium, thyroid, kidney and miscellaneous tumors.


Some personal recollections of two international congresses. Paris and Brussels in 1933 and London in 1937.


Oral cytology: its value and its limitations.


Treatment of diabetes mellitus with N-(P-chlorobenzensulfonyl)N'-pyrrolidinourea (deamelin-S)-with double-blind method.


The value of a cardiac resuscitation program in a community hospital.


The rod of comfort.

The rod of comfort.


Acute traumatic rupture of the thoracic aorta: report of a case with rupture of the innominate artery.


Chronic runt disease in rats: clinical and pathologic studies.


Arteries, atherosclerosis and periodontics.


Kallikreins and kinins.

Kallikreins and kinins.


Events occurring during the replication of bacteriophage T4 DNA. Ten temperature-sensitive mutants of T4D have been examined to find the times in the lytic cycle at which a shift to a restrictive temperature no longer exerts a lethal effect. Nine of these mutants play a role in phage DNA synthesis. The results of these experiments suggest that the products of three of the genes tested-genes 42, 44, and 45-have control or complexing functions in addition to any enzymatic ones. The data also indicate that the products of genes 30 and 41 may act cooperatively. The properties of mutants of gene 43 (DNA polymerase) and gene 56 (dCTP-dUTPase) suggest that the production of a certain length of concatameric DNA is necessary before viable phage can be produced.


The L-arabinose operon in Escherichia coli B-r: a genetic demonstration of two functional states of the product of a regulator gene. The product of the regulator gene araC in the L-arabinose gene complex exists in two functional states: P1, the repressor, and P2, the activator, presumably in equilibrium with each other, and with P1 and P2 attached to their respective controlling sites, araO, the operator, and araI, the initiator. The controlling sites are linked in the following order with respect to genes araB and araC: BIOC. Two C gene deletions (Delta719 and Delta766) serve to define the newly described araO site, and to place it adjacent to the left end of the C gene. We have suggested that deletion 719 deletes, in addition to the C gene, part or all of the araO site. Deletion 766 leaves the araO site intact. Complementation analysis employing stable merodiploids indicates that the repressor-operator site function is epistatic over the activator-initiator site function. It is necessary both for activator (P2) to be present and for repressor (P1) to be absent at their respective controlling sites (araI and araO) for full expression of the L-arabinose operon.


On the mechanism of pepsin action.

On the mechanism of pepsin action. The problem of the mechanism of pepsin action is considered in relation to recent data on the kinetics and specificity of the enzyme, as well as the finding, reported here, that pepsin exhibits a deuterium isotope effect in the cleavage of a peptide bond. The kinetic parameters for the hydrolysis of the Phe(NO(2))-Phe bond of Gly-Gly-Gly-Phe(NO(2))-Phe-OMe by pepsin have been determined in H(2)O and in D(2)O. The finding of a significant deuterium isotope effect (k(H2O)/k(D2O) = ca. 2) supports the hypothesis that the catalytic mechanism of pepsin involves the participation, in the rate-limiting step, of a proton donor (probably an enzymic carboxyl group) in addition to an enzymic carboxylate group acting as a nucleophile.


Transfer RNA methylases during morphogenesis in the cellular slime mold. The enzymes that methylate tRNA were studied during the life cycle of the colonizing slime mold, Dictyostelium discoideum. Total and base specific tRNA methylase activities were determined in extracts from cells in morphogenetic synchrony. Eight hours after enforced aggregation, the total methylase capacity is reduced by about 40 per cent. The diminution appears to be due to the presence of inhibitors that do not inhibit the base specific enzymes to the same extent. There is a still greater diminution in enzyme activity in extracts of the mature fruiting bodies.


Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. The 23S twisted circular form of ColE(1) DNA has been isolated from Escherichia coli as a tightly associated DNA-protein complex with a sedimentation coefficient of approximately 24S. Treatment of this complex with pronase, trypsin, sodium dodecyl sulfate, Sarkosyl, or heat results in a conversion to a slower sedimenting form of 17S or 18S, as determined by centrifugation in neutral sucrose gradients. These treatments do not alter the sedimentation properties of noncomplexes supercoiled ColE(1) DNA even in the presence of the ColE(1)-protein complex. Electron microscopic analyses indicate that the decrease in sedimentation rate of the ColE(1)-protein complex after treatment with these various agents is largely owing to an induced transition of ColE(1) DNA from the supercoiled to the open circular state.


Polypeptide chain initiation in E. coli: isolation of homogeneous initiation factor E2 and its relation to ribosomal proteins. Previous work has shown that F(2), one of several ribosomal factors involved in polypeptide chain initiation, functions in the binding of formylmethionyl-transfer RNA (fMet approximately tRNA(f)) to a messenger RNA-ribosome complex. F(2) was isolated from 1.0 M ammonium chloride washes of E. coli Q13 ribosomes as a protein homogeneous on polyacrylamide gel electrophoresis at both pH 4.5 and 7.8. Its molecular weight is approximately 80,000. Comparison of electrophoretic patterns of ribosomal proteins from NH(4)Cl-washed and unwashed ribosomes and F(2), at pH 4.5, shows that F(2) corresponds to the slowest-moving component of the proteins derived from unwashed ribosomes. This component is missing from the NH(4)Cl-washed ribosomes. The activity of F(2) is stimulated by two additional factors, initiation factor F(1) and a factor(s) present in a narrow ammonium sulfate fraction of the ribosomal NH(4)Cl wash. The nature of the latter is unknown.


Leucine tRNA and cessation of Escherichia coli protein synthesis upon phage T2 infection. The involvement of tRNA in cellular differentiation has been tested by analyzing aminoacyl-tRNA of Escherichia coli after phage T2 infection. One or two minutes after infection, half of one of the five leucine tRNA components (Leu-tRNA(1), CUG responding) undergoes a drastic structural change which leads to inactivity of both leucine acceptor activity and codon response. Whether or not the modification causes cessation of host protein synthesis without inhibiting phage-specific protein synthesis has been examined by analyzing polysome-bound leucine tRNA of E. coli before and after the phage infection. The results presented in this paper indicate that the amount of Leu-tRNA(1) used after infection was greatly reduced as compared to that used in noninfected cells. Studies of the in vitro protein-synthesizing system show that T2 mRNA rarely contains the CUG codon. A mechanism by which host mRNA translation is inhibited by the phage infection is proposed from this available information.


Dogmatism, learning, and resistance to change: a review and a new paradigm.


Natal's first heart transplant operation. An outline of the nursing service organisation.


A continuum in surgical education: a proposal for maximal flexibility in career planning by selective experiences in medical school and graduate training.


A study of acute intolerance to ethionamide, including a comparison with prothionamide, and of the influence of a vitamin B-complex additive in prophylaxis.


Precision of assay of whole-body potassium in man. ANL-7461.


Personal reminiscences of the early history of the radium extraction industry in the U.S.A. ANL-7461.


Imaging and scanning. UCLA-12-686.

Imaging and scanning. UCLA-12-686.


Radioisotope scintiangiophotography of cerebral hemisphere lesions. UCLA-12-686.


Regional alveolar liquid diffusibility by lung scintigraphy. UCLA-12-686.


Regional pulmonary function studies in canine lung allografts. UCLA-12-686.


Short-lived radiopharmaceutical agents for sequential and conventional kidney scanning. UCLA-12-686.


Studies on the chemical basis of radiation sensitization of cells by 5-bromouracil substitution in DNA. UCLA-12-686.


Symptomatology of the subclavian steal syndrome.


The founding of the University of Michigan Hospital: an innovation in medical education.


Effect of beta-lactamase location in Escherichia coli on penicillin synergy. Resistance to ampicillin in Escherichia coli is due generally to the presence of a beta-lactamase (penicillinase). Resistant strains have been found to fall into two groups: those with high-level resistance (1,000 mug/ml or greater) and those with low-level resistance (8 to 250 mug/ml). Most of the high-level resistant organisms posses beta-lactamases whose synthesis is episomally mediated. These strains release penicillinase from the cell when they are subjected to osmotic shock. Low-level resistant strains do not release the enzyme with osmotic shock. High-level resistant strains are not susceptible to the synergistic action of a penicillinase-resistant penicillin with ampicillin. Seventy eight per cent of low-level resistant strains are susceptible to the synergistic action of ampicillin and oxacillin. The two types of beta-lactamases are similar in regard to most properties; both enzymes are subject to competitive inhibition by penicillinase-resistant penicillins. The difference in location in the cell might explain why only some strains of E. coli are susceptible to the synergistic action of penicillin combinations.


Effect of incubation temperature on T-agglutination typing of Streptococcus pyogenes. The temperature of incubation affected the typability of beta-hemolytic group A streptococci by T-agglutination tests. When strains could not be typed after routine incubation at 30 C, they were incubated at 22 to 25 C, and nearly a 10% increase in typability was achieved. The clinical source of the strains was related to their typability. Incubation at the lower temperature was required for successful typing of higher percentages of strains from the skin and other clinical sources than from the throat. Sixty per cent of the skin strains were represented by six serotypes. Of these, 53% of the strains required incubation at 22 to 25 C before they could be typed.


Inactivation of the bactericidal activity of human serum by liquoid (sodium polyanetholsulfonate). Four serum-sensitive strains of Escherichia coli were exposed to 10, 20, and 50% fresh, heat-inactivated, and fresh human serum to which had been added Liquoid at a final concentration of 0.05, 0.025, 0.0125 and 0.006%. It was found that 50% fresh serum (in nutrient, Mueller-Hinton, thioglycolate, or Trypticase Soy Broth) killed more than 10(4) organisms/ml within 3 min, whereas 20 and 10% fresh serum required up to 20 and 40 min, respectively, to kill a comparable number of organisms. To neutralize the activity of 50% fresh serum, 0.0125% Liquoid had to be added, whereas an 0.006% final concentration of Liquoid was sufficient to antagonize the activity of 10 and 20% serum. However, when exposing extremely small bacterial inocula to fresh serum, at least 0.025% Liquoid was necessary to abolish the serum-bactericidal activity of 20 and 50% fresh serum. Liquoid had to be added to 50% fresh serum within seconds to prevent the killing of the majority of test organisms derived from small inocula. It is recommended that blood samples drawn from septicemic or bacteremic patients be aseptically added to a suitable broth which contains at least 0.025% Liquoid in order to improve the chances of isolating pathogens present in small numbers.


Evaluation of a washing procedure in the examination of almonds for Escherichia coli. Almond samples were examined for Escherichia coli by the EC method employing two sample preparation procedures: direct inoculation and washing. The washing procedure was conducted under specified conditions with an eluent containing surfactant; results averaged only 60% of the incidence shown by the direct inoculation procedure.


Nursing at University of Michigan Hospital: some observations and predictions.


Death of Salmonella typhimurium and Escherichia coli in the presence of freshly reconstituted dehydrated garlic and onion. The kinetics of the decline of populations of Salmonella typhimurium inoculated into freshly reconstituted dehydrated onion and garlic powders was studied. Measurable bactericidal activity was observed for onion and garlic concentrations of 1 and 5% (w/v), respectively, with maximal death rates occurring for concentrations of 5 and 10%. At these concentrations, the decimal reduction times were 1.1 and 1.2 hr, respectively, for resting cell cultures and 1.8 and 2.1 hr, respectively, for growing cultures. Of the major volatile aliphatic disulfide compounds of onions, n-propyl allyl and di-n-propyl, at concentrations of 0.1%, showed a comparable activity against resting cells but only a bacteriostatic effect toward actively growing cultures, which overcame this effect in 2 to 6 hr. At comparable concentrations, growing cultures of Escherichia coli were as susceptible to garlic, but apparently more resistant to onion, than were those of S. typhimurium.


Evaluation of two spot-indole reagents.

Evaluation of two spot-indole reagents. Two spot-indole reagents, p-dimethylaminobenzaldehyde (DMABA) and p-dimethylaminocinnamaldehyde, were evaluated quantitatively. Although fourfold less sensitive, DMABA proved to be more stable and economical.


Paul Crossland, MD. (1904-1968).

Paul Crossland, MD. (1904-1968).


History of the archives--a journal of ideas and ideals.


A clinical study of the behavioral effects of droperidol in the postoperative period.


Air pollution bibliography.

Air pollution bibliography.


The hormonal response to physical exercise.


A study of prosthetic dentistry.

A study of prosthetic dentistry.


William Goodwin Senior, C.B.E., PH.D., F.D.S.


One-stage reconstruction of pharynx and cervical esophagus with chest flap.


Scurvy in ancient Mesopotamia.

Scurvy in ancient Mesopotamia.


Immunofluorescence studies on the erythrocytic and sporogonic stages of the malaria parasite: stippling in infected erythrocytes.


Non-agglutinable vibrios isolated in the 1966 epidemic of cholera in Irag.


The Golden State Medical Association. The California Chapter of the National Medical Association.


Tetracycline-based histological analysis of bone remodeling.


Climatology and medicine. Article 3.

Climatology and medicine. Article 3.


History of sphygmology and of heart block.


Portal hypertension--current status.

Portal hypertension--current status.


Some recent work on the effects of carcinogens on nucleic acids with reference to hypothetical molecular mechanisms of carcinogenesis.


Toxicology of cycasin.

Toxicology of cycasin.


Genetics of host-controlled restriction and modification in Escherichia coli.


Codeine and its alternates for pain and cough relief. 2. Alternates for pain relief. This report-the second of a series on codeine and its alternates for pain and cough relief-contains a detailed evaluation of experimental and clinical data on newer substances having analgesic properties comparable to and in approximately the same range as those of codeine. The data are discussed under the headings: analgesic effects in animals; clinical usefulness; side-effects with particular reference to dependence and abuse liability.


Planned audit of medicare and medicaid payments.


An appraisal of sewage pollution along a section of the Natal coast.


Medical Association of Georgia.

Medical Association of Georgia.


President maine medical association. 1969-1970.


Medicarithmetic.

Medicarithmetic.


On the existence of independently melting subunits in a copolymeric DNA and their statistical distribution.


Release of free fatty acids following trauma.


Pulmonary complications of non-thoracic trauma: summary of the national research council conference.


Bacteriophage-induced inhibition of host functions. II. Evidence for multiple, sequential bacteriophage-induced deoxyribonucleases responsible for degradation of cellular deoxyribonucleic acid. Degradation of bacterial deoxyribonucleic acid (DNA) after infection with T4 bacteriophage was studied in an endonuclease I-deficient host. The kinetics of degradation were similar to those seen in other hosts with a normal level of this enzyme. Irradiation of extracellular phage with ultraviolet (UV) destroyed the capacity of the infecting virus to induce extensive breakdown of host DNA, which was, however, converted to high-molecular-weight material. Addition of chloramphenicol to T4-infected cells provided data which can be interpreted to indicate the involvement of at least two endodeoxyribonucleases and one exodeoxyribonuclease having a high degree of specificity. A model is proposed showing the sequential action of two endodeoxyribonucleases followed by an exodeoxyribonuclease in the degradation of host DNA. The appearance of these hydrolytic enzymes requires protein synthesis. Infections leading to partial degradation only (UV-irradiated phages, gene 46 mutants) effectively inhibited the synthesis of bacterial messenger ribonucleic acid and of beta-galactosidase.


Nature of Escherichia coli B(P1) yielder cells at the time of infection with restricted T1. In the infection of Escherichia coli B(P1) with restricted T1, it was shown that yielder cells consist of both special and nonspecial cells. Special or predetermined yielders occurred only among the earliest yielders. In most instances, yielder-cell formation was most easily explained by assuming that the first step was a chance escape of the restricted phage DNA from the degrading enzyme of the restricting cell.


Bacteriological water quality of several recreational areas in the Ross Barnett Reservoir.


Swift's half-way house.

Swift's half-way house.


Medical Society of the State of North Carolina. Committees & commissions appointments.


Robert Recorde, M.D. (1510?-1558). Tudor physician, mathematician, and pedagogue.


Criteria for intrauterine fetal transfusion.


Officers Southern Medical Association 1968-1969.


Prophylactic effect of nitrofurantoin (furadantin) in urologic procedures.


Horace H. Hayden (1769-1884). Father of professional dentistry.


Diagnosis and management of renovascular hypertension.


A review of the anatomy and neurophysiology of the opossum (Didelphis virginiana) visual system.


Contact lens glued to Bowman's membrane: a review.


The evolution of clinical psychiatry.

The evolution of clinical psychiatry.


Lawrence Coleman Kolb, M.D., ninety-seventh president, 1968-1969.


History of biological psychiatry in America.


History of the community psychiatry movement.


The evolution of psychiatric education.

The evolution of psychiatric education.


De humanitate.

De humanitate.


Renal allotransplantation in man. II. Transplantation in cystinosis, a metabolic disease.


Reassessment of operative procedures for portal hypertension in infants and children.


One layer end-on intestinal anastomoses using fine monofilament steel sutures.


Cardiac transplantation in man. 3. Surgical aspects.


Management of occlusive lesions of the branches of the aortic arch.


Membership directory. The American society of tropical medicine and hygiene.


The gastric ulcer problem.

The gastric ulcer problem.


Polypeptide hormones and calcium metabolism.


Isolation techniques for pharmacologically active substances (animal).


Hepatic sepsis after liver transplantation in dogs and pigs.


Penetrating wounds of the neck. A military and civilian experience.


Venous arterialization (fistula) for vascular access (hemodialysis).


William E. Platt, M.D.

William E. Platt, M.D.


Short-term in situ perfusion of the canine kidney following death.


A method f the estimation of nanogram amounts of cobamides and cobinamides in sheep rumen contents.


Ethnic differences in the incidence of malformations following migration.


Prof. Edward Zebrowski, pioneer in the study of the adverse effects of tobacco on the cardiovascular system.


Risk of anenceply in migrant and non-migrant women in the Oxford area.


Henryk Hoyer, M.D. 1834-1907.

Henryk Hoyer, M.D. 1834-1907.


Clinical aspects of temporal lobe epilepsy--a review. Temporal lobe epilepsy appears to be of increasing concern to psychiatrists, pediatricians and lawyers as an explanation for some aspects of behavior. There is a high incidence of structural abnormality associated with temporal lobe epilepsy, the most common being mesial temporal sclerosis. Depth electrode studies have shown that the most common focus for the clinical seizure is in the hippocampus or hippocampal gyrus. The first line of treatment continues to be attempt at control with anticonvulsant agents. Surgical treatment can be offered, in many instances, when drug therapy has failed.


Experimental nephritis due to type specific streptococci. V. Attempted use of tissue cultures and mice for assay of nephrotoxic streptococcal polypeptides.


Experimental nephritis due to type specific streptococci. VI. Further characterization of type 12 nephrotoxin.


Gamma-irradiation resistant mutants of Escherichia coli: modified enzyme synthesis repressible by arginine and uracil.


Isolation and characterization of an Escherichia coli strain harboring three sex factors.


Perspectives in adrenergic beta-receptor blockade.


Statistical ward rounds--15.

Statistical ward rounds--15.


Fine structural evidence for hormone secretion by a human thymic tumour. An epidermoid type of thymoma from an asymptomatic 39-year-old male subject is described. Electron microscopy demonstrated endocrine type secretory granules within the tumour cells, and a minority of these granules resembled normal human pancreatic alpha cell granules. Fine structural evidence for endocrine secretory activity in a primary thymic tumour has not been previously described. There are few fine structural reports of primary thymic tumours in man and to our knowledge these do not include certain features of the case described here.


Adenomatoid hamartoma of the lung in a neonate. A case of adenomatoid hamartoma in a premature male infant is described and the recent literature is reviewed. The present case demonstrates the frequently associated features of maternal hydramnios of late onset, and premature delivery with foetal respiratory difficulty and anasarca.


A modification to a radioisotopic dilution technique for vitamin B12.


C-reactive protein in malaria.

C-reactive protein in malaria. C-Reactive protein has not been studied in malaria so far but with the reappearance of malaria in the southern part of Iran there is now an opportunity to study the behaviour of C-reactive protein in malaria. One hundred and sixty-two patients suffering from malaria have been so tested. Of those patients, 85.2% showed a positive result and among them more than 50% had a strongly positive reaction. It may be concluded that malaria is one of the outstanding diseases in which the C-reactive protein test is positive. Those patients who were examined for C-reactive protein in the first two days of the disease showed a negative result.


Chloroquine-resistant P. falciparum infections.


In Memoriam. James Davidson Stuart Cameron, CBE, TD 1900-1969.


The Guinea trade and some of its surgeons (with special reference to the Royal College of Surgeons of Edinburgh).


Genetically determined defects in host resistance to infection: cellular immunologic aspects.


Infection and the spleen: association between hyposplenism, pneumococcal sepsis and disseminated intravascular coagulation.


Subclinical urinary infection in pregnancy.


Man as an experimental animal.

Man as an experimental animal.


Pulmonary disease related to Mycobacterium xenopei.


The health of Brunswick in the second half of the 19th century.


Primary lipid disorders. Clinicopathologic correlations.


Vitamin C intakes in Great Britain.

Vitamin C intakes in Great Britain.


Gastric and pancreatic function in pellagra.


Preparation for and recovery from anesthesia.


Modern trends in leprosy.

Modern trends in leprosy.


Recent investigations on histones and their functions.


Calcium ion and muscle contraction.

Calcium ion and muscle contraction.


The replication of RNA-containing bacteriophages.


Cell population kinetics in normal and malignant tissues.


Membrane models with phospholipids.

Membrane models with phospholipids.


The literature of medicinal chemistry.

The literature of medicinal chemistry.


Fungicidal action of organophosphorus compounds.


The fungitoxic mechanisms in quinoline compounds and their chelates.


Pentachlorobenzyl alcohol, a rice blast control agent.


Mode of action of natural insecticides.

Mode of action of natural insecticides.


Photochemical degradation products of pentachlorophenol.


Specificity and mechanism in the action f saligenin cyclic phosphorus esters.


Reactions of pesticides in soils.

Reactions of pesticides in soils.


Comparative mechanisms of insecticide binding with nerve components of insects and mammals.


Mode-of-action of photosynthesis inhibitor herbicides.


The strategy of finding fungicides.

The strategy of finding fungicides.


Retrospective aspects of pulmonary tuberculosis in Finland.


Memoir of Joseph Howard Cloud. 1872-1968.


Vaginal aplasia.

Vaginal aplasia.


Memoir of Matthew S. Ersner. 1890-1968.

Memoir of Matthew S. Ersner. 1890-1968.


Memoir of John Arthur Daugherty. 1902-1968.


Memoir of Archer P. Crosley, Jr. 1920-1968.


Memoir of Andrew J. Donnelly. 1910-1968.


Memoir of Francis Clark Grant. 1891-1967.


Memoir of William E. Krewson, 3d. 1908-1968.


Memoir of William Harvey Perkins. 1894-1967.


Memoir of William Whitaker. 1880-1968.

Memoir of William Whitaker. 1880-1968.


Edwin J. Cohn lecture Edwin J. Cohn and protein chemistry.


'Haptoglobin' after liver transplantation in dogs.


Paul Moureau (1904-1968).

Paul Moureau (1904-1968).


Secondary retinal detachment. Electron microscopy of retina and pigment epithelium.


Treponemalike forms and artifacts.

Treponemalike forms and artifacts.


Interstitial keratitis and glaucoma.

Interstitial keratitis and glaucoma.


Instrumentation and techniques for refractive keratoplasty.


Recent advances in the functional anatomy of the upper limb.


Aortic valve replacement with autogenous tissue.


Control of thromboembolism from prosthetic heart valves.


Atrial pacing in the postoperative management of cardiac homotransplantation.


New directions in information resources for industrial hygiene and occupational health.


Cardiac pacemaking.

Cardiac pacemaking.


Raising a great dust and complaining we cannot see.


Death from ruptured lung without fracture of any ribs in a young man. A case report.


Relations between Dr. Jackson of America and Geminiano Grimelli of Modena concerning ether anaesthesia.


Hypertensive encephalopathy following anaesthesia.


Selection of patients for pulmonary embolectomy.


Alfred Soffer, M. D.

Alfred Soffer, M. D.


Roster of associate members--1969.

Roster of associate members--1969.


Radioisotope scanning in internal medicine.


The stomach's acid.

The stomach's acid.


1969 roster of the Greater St. Louis Dental Society.


Elda Emma Anderson.

Elda Emma Anderson.


Pharmacology of anesthetic agents.

Pharmacology of anesthetic agents.


Relative absorption of both tetracycline and penicillin G administered rectally and orally in aqueous solution.


Fenfluramine: study of its central action through its effects on sleep.


Penetration of antibiotics into the cerebro-spinal fluid in inflammatory conditions. 1. Preface and comparative study of ampicillin with hetacillin.


Why care about medicare?

Why care about medicare?


The lady doctor of the grove.

The lady doctor of the grove.


Hospitals and the practice of medicine in Spanish Pensacola.


Anesthesia in fishes.

Anesthesia in fishes.


The Pensacola campaign through a nurse's eye.


Inhalation anesthesia in the dog.

Inhalation anesthesia in the dog.


The link between Melanchthon and Vesalius.


The effect of calcium chloride and formaldehyde on the release and composition of phospholipids from cryostat sections of rat hypothalamus.


Roster of members, Kansas State Dental Association as of July 1, 1969.


"High speed and oral surgery".

"High speed and oral surgery".


Cribs like castings.

Cribs like castings.


Isolation of Salmonellae from New Guinea streams and waterholes using an elevated temperature technique.


Bacterial sporulation as a modified procaryotic cell division.


Crystals of fragment Fab': preparation from pepsin digests of human IgG myeloma proteins.


Breaks in DNA stimulate transcription by core RNA polymerase.


Dialog in treatment of dental pain.

Dialog in treatment of dental pain.


Ethacrynic acid. A non-diabetogenic diuretic.


Editorial: the crumbling image.

Editorial: the crumbling image.


Magnus as scientist and teacher.

Magnus as scientist and teacher.


Rudolf Magnus. Personal reminiscences.

Rudolf Magnus. Personal reminiscences.


Regulation of LH secretion in the rat.

Regulation of LH secretion in the rat.


The effect of propranolol on exercise-induced ischemic S-T segment depression.


Mechanisms in reciprocal rhythm.

Mechanisms in reciprocal rhythm.


Schoenlein on hydrothorax (1832).

Schoenlein on hydrothorax (1832).


Infectious bovine rhinoracheitis virus in bovine kidney cells: sequence of viral production, cellular changes, and localization of viral nucleic acid and protein.


Pathogenesis of African swine fever in young pigs.


Effects of sublethal concentrations of penicillin G on groups C, E, K, and L streptococci.


An investigation of possible synergism between flufenamic acid and prednisone.


Flufenamic acid and indomethacin in rheumatoid arthritis.


A comparison of flufenamic acid and phenylbutazone in osteoarthritis of the hip.


Mefenamic acid compared with indomethacin and placebo in osteoarthritis.


A controlled study of flufenamic acid in ankylosing spondylitis. A preliminary report.


Experimental observations on flufenamic, mefenamic, and meclofenamic acids. IV. Toleration by normal human subjects.


Effects of the fenamates on the threshold of pain.


Clinical trials of the fenamates. The value of computers in analysing clinical trials.


Evaluation of mefenamic acid and flufenamic acid in clinical pain.


Review of flufenamic acid in rheumatoid arthritis.


A controlled clinical trial of flufenamic acid in rheumatoid arthritis.


A clinical trial of flufenamic acid in the treatment of rheumatoid arthritis.


The toxicity of spermine and spermidine to cells in culture.


Studies concerning the mechanism of action of antimalarial drugs. Inhibition of the incorporation of adenosine-8-3H into nucleic acids of Plasmodium berghei.


4 (or 5)-diazoimidazole-5 (or 4)-carboxamide and related triazenoimidazoles as antibacterial agents: their effects on nucleic acid metabolism of Escherichia coli B.


The investigation of nucleic acid secondary structure by means of chemical modification with a carbodiimide reagent. II. The reaction between N-cyclohexyl-N'-beta-(4-methylmorpholinium)ethylcarbodiimide and transfer ribonucleic acid.


The free energy of hydrolysis of the phosphoryl-enzyme intermediate in alkaline phosphatase catalyzed reactions.


Purification and properties of asparaginase from escherichia coli B.


Quantitative appraisals of possible catalytic intermediates in the succinyl coenzyme A synthetase reaction.


On the reported presence of biotin in carbamyl phosphate synthetase.


Dephosphorylation of succinyl coenzyme A synthetase as related to enzyme specificity and catalytic intermediates.


A topically applied salicylate gel. A controlled trial and subjective assessment of pain relief in oral lesions.


Transplantable Friend virus-induced tumors in rats.


Experimental evidence for the existence of an extrarhinencephalic control of the activity of the cobalt rhinencephalic epileptogenic focus. Part 1. The role played by the caudate nucleus.


Rage: a paraepileptic phenomenon?

Rage: a paraepileptic phenomenon?


Further considerations of delays in the oculomotor pathways.


Triazine nucleic acid interrelationship in Escherichia coli 15 arg-t-u.


Structure of nuclear membranes isolated from brain cells.


Antibody production in cultured lymphoblastoid cell line.


A microscopic test for rapid detection of antibodies against Mycoplasma pneumoniae.


Regenerating bone-marrow cell lines in two different X-irradiation conditions.


Electron-affinic sensitization. I. A structural basis for chemical radiosensitizers in bacteria.


Thiamine-induced formation of the monopyrrole moiety of prodigiosin. Thiamine stimulates the production of a red pigment, which is chromatographically and spectrophotometrically identical to prodigiosin, by growing cultures of Serratia marcescens mutant 9-3-3. This mutant is blocked in the formation of 2-methyl-3-amylpyrrole (MAP), the monopyrrole moiety of prodigiosin, but accumulates 4-methoxy-2,2,'-bipyrrole-5-carboxaldehyde (MBC) and can couple this compound with MAP to form prodigiosin. Addition of thiamine caused production of MAP, and as little as 0.02 mg of thiamine per ml in a peptone-glycerol medium stimulated production of measurable amounts of prodigiosin. Phosphate salts and another type of peptone decreased the thiamine-induced formation of prodigiosin; yeast extract and glycerol enhanced the formation of this substance. Thiamine also enhanced production of prodigiosin by wild-type strain Nima of S. marcescens. The thiamine antagonists, oxythiamine and pyrithiamine, inhibited thiamine-induced production of MAP and of prodigiosin by the mutant strain 9-3-3, formation of prodigiosin by the wild-type strain Nima, and production of MAP by another mutant, strain WF. The pyrimidine moiety of thiamine was only 10% as effective as the vitamin; the thiazole moiety, only 4%; and the two moieties together, 25%. Various other vitamins tested did not stimulate formation of prodigiosin by strain 9-3-3. Thiamine did not stimulate production of prodigiosin by a single-step mutant that showed the same phenotypic block in prodigiosin biosynthesis as strain 9-3-3. This is not surprising since strain 9-3-3 originated as a result of two mutational events. One event may involve thiamine directly, and the other may involve the biosynthesis of MAP. Thiamine is probably involved in the regulation of the biosynthesis of MAP, because the vitamin or inhibitory antagonists must be added during the early phases of growth in order to be effective.


Production of the Shwartzman reaction with microbial L forms. Study of potential pathogenicity of microbial L forms was done by the localized Shwartzman reaction. Stable L forms of Proteus mirabilis served as skin preparation in rabbits for induction of Shwartzman reaction by subsequent intravenous injection of either P. mirabilis L forms or Escherichia coli endotoxin. The intensity of the reaction was positively correlated to numbers of L forms in the skin. L forms also served as the intravenous challenge. In vivo multiplication of L forms was not a prerequisite for the reaction, as it could be produced with nonviable, osmotically lysed L forms. The reaction produced with L forms in the skin was more intense than that produced with the parent bacterial form. These latter observations, coupled with the demonstration that L forms disappeared from the skin (lysed?) after 4 hr, in contrast to bacteria which were recoverable for 72 hr (duration of study), suggest release of endotoxin by L forms as a pathogenic mechanism.


Spontaneous lethal sectoring, a further feature of Escherichia coli strains deficient in the function of rec and uvr genes. Eight recombination-deficient (Rec(-)) mutants of Escherichia coli were studied. Progeny lines were obtained on solid media, by means of micromanipulation, and the colony-forming ability of individual cells was analyzed. Cells of all eight strains gave rise to colony-forming as well as non-colony-forming descendants ("lethal sectoring"). Lethal sectors, i.e., groups of non-colony-forming cells which originate from a common ancestor, appeared with frequencies per generation ranging between 4 and 20% in Rec(-) strains, whereas lethal sectors were rare in Rec(+) strains (less than 1%). A strain carrying a mutation (uvrA6) in one of the genes involved in pyrimidine dimer excision from deoxyribonucleic acid (DNA) showed twice as many lethal sectors per generation as a strain with the genotype uvrA(+). Similarly, a double mutant (AB2480, uvrA6, recA13) showed twice as much spontaneous lethal sectoring as the corresponding Rec(-) strain (uvrA(+), recA13). The kinetics of growth curves obtained in nutrient broth and the frequency of non-colony-forming units in stationary-phase broth cultures indicate clearly that lethal sectors occur in liquid cultures too. The causes for spontaneous lethal sectoring are unknown at present. It seems reasonable to assume that gene uvrA and the rec genes are somehow involved in the repair of spontaneously occurring DNA lesions, since a deficiency in this type of repair may cause lethal sectors. The extent to which spontaneous lethal sectoring (observed in all Rec(-) strains of E. coli studied) may contribute indirectly to the failure to form recombinants is discussed.


Pyrogenic principle of the ribonucleic acid from the yeast Candida utilis. Experiments were performed to characterize the pyrogenic principle of ribonucleic acid (RNA) from the yeast Candida utilis. It was shown that ribonuclease hydrolysis of the RNA does not lead to inactivation of the pyrogenicity. Pyrogenicity was, however, destroyed by treatment with sodium deoxycholate. On column chromatography with Biogel under sterile and pyrogen-free conditions, the pyrogenic principle of yeast RNA was eluted together with the RNA. After treatment of the RNA with ribonuclease, it was possible to separate the pyrogenic activity from the RNA (hydrolysis products) to a great extent. Column chromatography of Escherichia coli endotoxin showed that the endotoxin was eluted in the same fractions as the pyrogenic activity of yeast RNA. On the basis of the behavior of the pyrogen, it may very well be that the fever reaction is produced not by the nucleic acid but by pyrogenic contaminants of the RNA preparation.


Coresistance to neomycin and kanamycin by mutations in an Escherichia coli locus that affects ribosomes. Mutant strains resistant to neomycin or to kanamycin sulfate were isolated from Escherichia coli K-12. Nine mutants were analyzed; all were resistant to both antibiotics (about 150 and 100 mug/ml, respectively), and were designated nek. In the mutant strains, the ribosomes are changed from those of the parental strain; for when they were used in assays for polypeptide formation directed by polyadenylic acid or polycytidylic acid, coding fidelity in presence of the drugs was increased and inhibition of synthesis by the drugs was lessened. Mating experiments and transduction tests showed that all of the nine nek mutants are either closely linked or allelic, and the nek locus is closely linked to two genes-str (streptomycin) and spc (spectinomycin)-known to affect the 30S ribosome. The two nek mutants tested were recessive to the sensitive, wild-type allele. When the nek mutants were compared to the parental strain, pleiotropic effects of the nek mutations were observed. Resistance to low levels of streptomycin and spectinomycin was increased, whereas resistance to chloramphenicol was decreased. Also, the mutants were less able to adapt to high concentrations of lincomycin, and could no longer show phenotypic suppression of an arginine requirement by neomycin or kanamycin. Such pleiotropic effects are suggested to be the rule for mutations in genes that participate in the biosynthesis of a cellular organelle.


Induction by oxygen of respiration and phosphorylation of anaerobically grown Escherichia coli. The changes occurring in the respiratory enzymes of anaerobically grown Escherichia coli strain B and E. coli 15 T(-)A(-)U(-)bar during exposure to oxygen were studied. Reduced nicotinamide adenine dinucleotide (NADH) oxidase activity reached its peak soon after O(2) exposure; cytochrome content and succinate oxidase activity increased more slowly, and these increases paralleled each other. The activities of isocitrate and malate dehydrogenases also increased, but the increase was less than that of the succinate and NADH oxidases; exposure to O(2) had no effect on the succinate and NADH dehydrogenase activities. On the other hand, the glycolytic activity decreased slowly after O(2) exposure. The incorporation of (32)P into acid-soluble organic phosphate esters paralleled the respiratory rate during the first 60 min after O(2) exposure, but continued to increase after the respiration reached a plateau. The sensitivity of (32)P incorporation to the uncoupler carbonyl cyanide m-chlorophenylhydrazone also increased with time. The observed relationship between the development of the respiratory chain and the energy-conserving mechanism during O(2) exposure is discussed. Synthesis of the respiratory enzymes upon exposure to oxygen was dependent on concomitant protein and ribonucleic acid synthesis but not on deoxyribonucleic acid synthesis.


Cell wall composition and serological reactivity of Histoplasma capsulatum serotypes and related species. The composition of the cell walls of strains of Histoplasma capsulatum that were reactive in the complement-fixation test and that reacted with fluorescein-tagged specific anti-H. capsulatum serum were compared with the cell walls of H. capsulatum and Histoplasma duboisii that did not react in these serological tests. The cell walls of the serologically reactive strains of H. capsulatum were much more susceptible to the action of proteinases and to chitinase than were the serologically nonreactive strains, but were less susceptible to hydrolysis by 3% NaOH or 2 n H(2)SO(4). The walls of the serologically reactive strains had higher chitin, amino acid, and hexosamine contents, but they were lower in a "lactic acid" component that was released by alkaline hydrolysis of the nonreactive strains. The cell walls of strains of H. duboisii were similar to the walls of the serologically nonreactive strains of H. capsulatum. Chemical analyses of the cell walls of the various strains clearly support the serological typing of H. capsulatum and H. duboisii strains reported by Kaufman and Blumer.


The computer--concept, development and problem environment.


Computers in medicine--a personal perspective.


Interaction of colicins with bacterial cells. IV. Immunity breakdown studied with colicins Ia and Ib. Colicinogenic cells are immune to the lethal effect of the colicin which they produce. In the presence of very high concentrations of colicin, however, colicinogenic cells are no longer immune to the homologous colicin. This phenomenon, immunity breakdown, was studied with colicins Ia and Ib. The biochemical effects of colicin Ib on Escherichia coli were studied with a standard noncolicinogenic strain. At multiplicities of about 10 or higher, colicin Ib inhibited incorporation of leucine into protein and incorporation of (32)P-inorganic phosphate into deoxyribonucleic acid and ribonucleic acid by more than 95%. Under the same conditions, (32)P incorporation into phospholipid and nucleotide fractions was inhibited only partially (about 80 and 60%, respectively). Inhibition of (32)P incorporation into the terminal phosphorus of adenosine triphosphate was also considerably less than that of macromolecular synthesis (50 to 60%). (32)P incorporation into the nonnucleotide organic phosphate fraction was not inhibited. Respiration was not affected. Colicin Ia showed the same biochemical effects as colicin Ib. A mutant of an Ib-colicinogenic E. coli strain selected for resistance to low concentrations of colicin Ia was shown to be resistant to high concentrations of homologous colicin Ib, whereas the parent Ib-colicinogenic strain is sensitive to high concentrations of colicin Ib. This mutant lost its specific receptors for colicin Ib. Moreover, the biochemical effects of high concentrations of colicin Ib on Ib-colicinogenic cells during immunity breakdown were similar to the effects found in sensitive cells exposed to low concentrations of the same colicin. It is concluded that the killing of colicinogenic cells in the presence of high concentrations of homologous colicin is indeed caused by the homologous colicin molecules.


Computer-aided analysis of radiographic images.


S.A. Tissot (1728-1797). Layman's guide to health.


Chromosomal abnormalities in certain human malignancies. A review.


Captain James Cook (1728-1779).

Captain James Cook (1728-1779).


Argyrophil (kinin and amine producing?) cells in human infant airway epithelium.


Radiopotassium equilibrium in total body potassium: studies using 43K and 42K.


Vascular symptomatic relief during administration of ethylchlorophenoxyisobutyrate (clofibrate).


Renal clearance of amylase during pancreatitis.


Glycylproline peptiduria in familial hyperostosis of obscure nature.


Total thigh flaps for extensive decubitus ulcers. A 16 year review of 41 total thigh flaps.


Rotation flaps of the nose.

Rotation flaps of the nose.


An account of two successful operations for restoring a lost nose.


Early management of the burned ear.

Early management of the burned ear.


The fat flip flap. A method of blending a pedicle implant.


E. Fulton Risdon (1880-1968).

E. Fulton Risdon (1880-1968).


The Institute of Medicine of Chicago and federal health programs.


Development of personality and emotional-social adjustment in deaf and partially hearing children.


Sonne dysentery. Some observations on a food-poisoning incident during an epidemic and on the development of resistance to nalidixic acid.


Myocardial vascular morphology and capillary blood flow after mammary artery implantation in dogs.


'The Sheens and the Welsh National School of Medicine'.


The world distribution of transferrin variants and some unsolved problems.


Karl Landsteiner. Father of blood grouping and immunochemistry.


Inhibition of granuloma formation around Schistosoma mansoni eggs. V. "Hodgkin's-like lesion" in SJ J mice.


The nose as form and function.

The nose as form and function.


Upper airway problems in severely burned patients.


Clinical pharmacologic study of 1-acetyl-2-picolinoyl hydrazine (NSC-68626).


Procarbazine (NSC-77213) in the treatment of advanced bronchogenic carcinoma.


The hydrogen method of measuring local blood flow in subcortical structures of the brain: including a comparative study with the 14C antipyrine method.


Studies of avian visual pathways involved in cardiac conditioning: nucleus rotundus and ectostriatum.


The effect of fixation with formaldehyde and glutaraldehyde on the composition of phospholipids extractable from rat hypothalamus.


A simple replication technique for the study of biological tissues by electron microscopy.


The use of liquid photographic emulsion for demonstrating blood vessels in histological sections.


Immunohistochemical demonstration of gastrin in mammalian islets of Langerhans.


Biographic and personal recollections of George N. Papanicolaou.


Damage, repair, and recombination. I. Contribution of the host to recombination between the superinfecting phage and the prophage.


Semliki forest virus temperature-sensitive mutants: isolation and characterization.


Damage, repair, and recombination. II. Effect of hydrogen peroxide on the bacteriophage genome.


Electron micrographic maps of deletions and substitutions in the genomes of transducing coliphages lambda dg and lambda bio.


Fluorescent antibody staining of tobacco mosaic virus antigen in tobacco mesophyll protoplasts.


Cytochemical examination of lactic dehydrogenase and glucose-6-phosphate dehydrogenase in smears from the uterine cervix.


Atypia of the bronchial epithelium in wind instrument players and in singers: a cytopathologic study.


A new method for detecting changes in the surfaces of human exfoliated cervical cells with the scanning electron microscope.


Standardization of dental prosthetic materials.


Bacteriological sampling from root canals directly after chemomechanical treatment. A clinical and bacteriological study.


Opening remarks: quantium-mechanical calculations of biological structures and mechanisms.


Roentgenological and autopsy studies on the gas content of the lungs and gastro-intestinal tract in living and stillborn infants, and sources of error in resuscitation.


On the intestinal flora.

On the intestinal flora.


The effect of secretin, glucagon, and duodenal acidification on pepsin secretion in man.


The stereotaxic treatment of paedophilic homsexuality.


Group specificity in protein synthesis, 3.


Sterilisation of food handling utensils in ancient India.


Maternal rubella and the risk of congenital malformation in infants--a review.


Osma Journal--president's page.

Osma Journal--president's page.


Neurosurgical biblio-index. Citations from the Index Medicus March through May 1969.


Chemical aspects of penicillin allergy.

Chemical aspects of penicillin allergy.


Cardiac transplantation.

Cardiac transplantation.


Present status of lung transplantation.

Present status of lung transplantation.


Human cardiac transplantations. Recent observations.


Jon V. Straumfjord, M.D. 1899-1969.

Jon V. Straumfjord, M.D. 1899-1969.


Approaches to the molecular nature of pharmacological receptors.


Mutants in Escherichia coli ribosomes: a new selection. Only a few ribosome genes have been identified. Increasing the available kinds of ribosome mutants would thus facilitate the analysis of specification and function of the organelle. In an attempt to reveal a number of ribosome genes, we sought mutants that could not form functional ribosomes at a nonpermissive temperature (43 degrees ). Cells were depleted of ribosomes by nutrient starvation at 43 degrees , and 98 strains that could not recover from the starvation were then isolated. Of these 98, many satisfied auxiliary criteria for ribosome mutants; 23 showed increased sensitivity to antibiotics that affect ribosome function; and 8 of 20 representative strains had crude extracts more temperature sensitive than that of the parental strain when assayed for polyphenylalanine formation in the presence of excess soluble enzymes (S100) of the parental strain. In further tests with two of these strains, the temperature sensitivity of the washed ribosomes, compared to that of the parental strain, was confirmed. The variety of phenotypes among the mutants suggests that this selection is capable of revealing mutants in many ribosome genes.


Active transport of magnesium in escherichia coli. Magnesium-28 is accumulated by cells of Escherichia coli by a process that is temperature-dependent and inhibited by dinitrophenol and cyanide. The rate of uptake of magnesium is concentration-dependent, with a "K(m)" of about 5 x 10(-4)M in tryptone broth. There is evidence for cellular control over this rate. The magnesium accumulation system is specific, since neither potassium nor calcium (nor apparently manganese) competes with magnesium for cellular uptake. The magnesium that is accumulated by the cells can be released by treatment with toluene and partially exchanges with excess exogenous magnesium.


A mutant of Escherichia coli permitting replication of two F factors. Control mechanisms of replication of bacterial genetic elements are poorly understood at present. We have studied one such mechanism involving replication of the F factor in Escherichia coli. The F factor can replicate either autonomously in F(+) or F' strains, or as an integral part of the chromosome in Hfr strains. We have shown that presence of either an integrated F factor or a free F factor prevents replication of a second free F factor. Two integrated F factors can replicate in the same cell. The present experiments show that when a F'lac element was transferred by mating into an Hfr strain, it had to become integrated into the chromosome in order to persist. With a recombination-deficient (recA) Hfr strain as recipient, the frequency of F'lac integration was greatly reduced. This permitted us to isolate a mutant Hfr strain in which the F'lac element was able to replicate autonomously. The mutation has most likely occurred in the integrated F factor itself. Availability of this new recA mutant Hfr strain facilitates genetic analysis of the F factor, since in recA(+) Hfr strains frequent integration of a free F factor into the chromosome obscures recognition of complementation and recombination between two free F factors.


Origin and direction of DNA synthesis in E. coli B-r. Replication of the chromosome of E. coli B/r has been studied by measuring the change with cell age of the rates of reversion to wild type of auxotrophic markers induced by nitrosoguanidine. Synchronous populations of bacteria were obtained by the procedure of Helmstetter and Cummings. The origin of DNA synthesis was determined to occur at 49 minutes +/- 5 minutes on the genetic map, and the direction of synthesis was found to be clockwise.


Translocation of mRNA codons. II. Properties of an anti-translocase antibody. A purified preparation of translocase, one of several enzymes required for protein biosynthesis, has been used to prepare a specific anti-translocase antibody. This antibody provides an extremely useful tool not only for detecting the enzyme independent of its activity, but for inhibiting the translocase-mediated reaction and, thus, protein biosynthesis. Though the antibody very rapidly inhibits elongation of the peptide chain, it fails to effect initiation, binding, or peptide bond formation, which strongly suggests that translocase is not a direct participant in these reactions. It also arrests translation of the defined tricodon AUG(U(6)) at the second codon, permitting formation only of the dipeptide, fMet-Phe, rather than the tripeptide, fMetPhe-(Phe)(2), which is formed in the presence of control serum. We have shown previously that the third codon becomes available only in the presence of translocase, thereby defining the site of action of the antibody. The antibody also has been used to demonstrate that translocase is antigenically distinct from the other proteins required for initiation and protein synthesis in E. coli.


Synthesis of phage-specific alpha- and beta-glucosyl transferases directed by T-even DNA in vitro. -Bacteriophage T4 DNA, when added to a ribonucleic acid- and protein-synthesizing system from uninfected Escherichia coli, directed the in vitro synthesis of virus-specific glucosyl transferases. The T4-specific alpha- and beta-glucosyl transferases are synthesized in vivo early after infection, and function to glucosylate the hydroxymethylcytosine residues of phage DNA. The in vitro glucosyl transferase synthesis was dependent upon transcription of T4 DNA, as well as upon protein synthesis. DNA from T4 mutants unable to induce glucosyl transerases failed to induce enzyme formation in vitro, although protein synthesis was unimpaired.


Endonuclease II of E. coli. I. Isolation and purification. The isolation and purification of a new endonuclease of E. coli is described. This enzyme degrades alkylated DNA as assayed by a technique that requires double-strand scission. The enzyme also makes a limited number of single-strand breaks in native nonalkylated DNA.


Crystallization of DNA from dilute solution. Hexagonal platelet crystals about 1 mu across and about 150 A thick have been precipitated by the addition of ethanol to solutions of ultrasonically degraded, salmon sperm DNA in sodium cacodylate buffer. X-ray powder diffraction patterns from concentrated slurries of these crystals in mother liquor show intense rings at 23.9 A and at about 3.4 A; the former arise from lateral packing and the latter from an axial periodicity of the DNA helix. Evidence is presented to indicate that native DNA helices crystallize by chain folding and that no irreversible denaturation results from the crystallization process.


The molecular basis of suppression in an ochre suppressor strain possessing altered ribosomes. Escherichia coli K12 2320(lambda)-15B has a mutation that results in ochre suppressor activity.(1) This mutation concomitantly causes a decreased growth rate in rich medium, an increased sensitivity to streptomycin,(1) and the production of some altered 30S ribosomes which are differentially sensitive to RNase.(2) The results presented below demonstrate that the molecules which cause suppression are tRNA. These observations justify the conclusions that the suppressor mutation did not occur in a structural gene for a ribosomal component, and that the decreased growth rate in rich medium, the increased sensitivity to streptomycin, and the production of altered 30S ribosomes are probably all secondary consequences of the suppressor mutation.


The dominance of streptomycin sensitivity re-examined. Several aspects of the strA phenotype were studied in strains of Escherichia coli diploid in the strA chromosomal region. It was found that alleles causing different levels of interference with amber suppression can complement each other, the less restrictive effects being predominant. The sensitive strA(+) allele determines two responses to streptomycin: a dominant effect consisting of a sudden, complete, but reversible inhibition of growth, and a recessive effect manifested as cell killing. Both restriction of suppression and inhibition of growth reflect ribosomal involvement in translation.


Radiatio herapy of malignant bone tumors.


Suppression of reseed mechanism by prior irradiation in SJL-J mouse. Hodgkin's-like reticulum-cell sarcoma.


Mixed bacterial meningitis due to Haemophilus influenzae and Salmonella typhimurium.


Development and transformation of the aortic arches in the equine embryos with special attention to the formation of the definitive arch of the aorta and the common brachiocephalic trunk.


Electron microscopy after life state investigation of a cell in culture.


Endothelial microvilli in the vessels of the rat gasserian ganglion and testis.


[The fine structure of the organon vasculosum hypothalami of the frog (Rana temporaria)].


Electron microscopic studies on the adenohypophysis of the white-crowned sparrow, Zonotrichia leucophrys gambelii.


Possible sites of release of neurosecretory granules in the sinus gland of the crayfish, Orconectes nais.


Continuities between mitochondria and endoplasmic reticulum in the mammalian ovary.


Intranuclear filament bundles in the intestinal epithelium of the oligochete Dero obtusa.


The structural organization of the intracerebral giant fiber system of cephalopods. The chiasma of the first order giant axons.


The nerve fibres in the basement membrane and related structures in Saccoglossus horsti (Enteropneusta).


A review of the brain research carried out in the Department of Neurophysiology of the Nencki Institute of Experimental Biology.


Musculus articularis genus in human fetuses, newborn and young individuals.


The investigation of Xenopus laevis hemoglobins during development by a fluorescent antibody.


Influence of diuretics on renal calcium excretion.


Division of Entamoeba invadens Rodhain, 1934, in cultures.


Alexander Lee McGregor, M.B., Ch.B., Ch.M. (Edin.), F.R.C.S. (Eng.).


Differentiation of rabbit blastocysts in vitro.


A selected public health bibliography with annotations.


The Wolff-Parkinson-White syndrome.

The Wolff-Parkinson-White syndrome.


Glucagon and the cardiovascular system.

Glucagon and the cardiovascular system.


Medical writing and Webster's Third.

Medical writing and Webster's Third.


Georges Faroy (1881-1968).

Georges Faroy (1881-1968).


Serial measurement of the cortical blood flow in canine renal allograts during the rejection crisis.


Observations on investigations prior to schistosomiasis control.


C.A. Pannett, B.SC., M.D., F.R.C.S.

C.A. Pannett, B.SC., M.D., F.R.C.S.


Fenfluramine (Ponderax) in the treatment of obese psychiatric out-patients.


Methoxyflurane and nitrous oxide as obstetric analgesics. I. A comparison by continuous administration. Methoxyflurane and nitrous oxide have been compared as obstetric analgesics. The inhaled concentrations of these agents, given continuously, were adjusted by an anaesthetist to maintain each patient at the optimum state between reaction to pain and consciousness. Assessments were made continuously.Though the anaesthetist's assessment showed no difference between the mean results, a greater proportion of the methoxyflurane patients were "satisfactory" for 90-100% of the time than of the nitrous oxide patients, particularly in regard to objective pain relief. The midwives' opinion of those who had "complete" pain relief supported this. Nausea was significantly less among methoxyflurane patients, and vomiting during labour occurred only in patients who had nitrous oxide. It is concluded that nitrous oxide and methoxyflurane given in a continuously adjusted concentration are almost equally effective as obstetric analgesics, though there are certain features which favour methoxyflurane.


Field trial of methoxyflurane, nitrous oxide, and trichloroethylene as obstetric analgesics. In a field trial of 1,257 patients receiving methoxyflurane, trichloroethylene, and nitrous-oxide/oxygen for the relief of pain in labour methoxyflurane has been shown to have certain advantages which support its use in midwifery practice. The trial confirms our objective method for screening an inhalational agent as an obstetric analgesic.


Methoxyflurane and nitrous oxide as obstetric analgesics. II. A comparison by self-administered intermittent inhalation. Methoxyflurane (0.35%) in air and nitrous oxide/oxygen (50%/50%) self-administered intermittently in the usual way have been compared as analgesics for labour. There were 25 patients in each group. Objective assessment by an anaesthetist showed that methoxyflurane is the more effective analgesic, and this was supported by the opinion of the multiparae. Nausea and vomiting were significantly less with methoxyflurane. Fifty per cent. nitrous oxide in oxygen given intermittently does not appear to be the best analgesic concentration. Nevertheless, since a considerable variation in sensitivity exists, it would probably be unwise to consider the introduction of higher concentrations for use by unsupervised midwives.This trial confirms the predictions made by us using a method for screening inhalational analgesics, in which methoxyflurane and nitrous oxide were given continuously.


Effect of anti-inflammatory drug therapy on clearance of 133-Xe from knee joints of patients with rheumatoid arthritis. The degree of joint inflammation in 13 patients with rheumatoid arthritis, as measured by clinical indices, was reduced by both sodium salicylate and indomethacin. The clearance rate of (133)Xe was reduced by indomethacin alone, and showed no correlation with the clinical features.


Presentation of the Academy plaque to Peter M. Murray, M.D.


Presentation of the Academy medal to Harold W. Brown, M.D.


Guillaume Dupuytren: his character and contributions.


Forty-four years of correspondence between Eugen Steinach and Harry Benjamin. A valuable addition to the Manuscript Collection of The Library of The New York Academy of Medicine.


The great Boston hernia controversy concerning the permanent cure of reducible hernia or rupture.


Trimethoprim and sulphamethoxazole in the treatment of chronic chest infections.


The herbal as a medical document.

The herbal as a medical document.


The Uünfbilderserie reconsidered.

The Uünfbilderserie reconsidered.


Historicism in medical history.

Historicism in medical history.


"Madness and its myths" an inaugural lecture delivered at Makerere University College on 1st February, 1968.


Synergistic activity of ampicillin and cloxacillin. Protective effect of cloxacillin on enzymatic degradation of ampicillin by penicillinase, and therapeutic activity of mixtures of ampicillin and cloxacillin.


Screening of antiviral antibiotics from actinomycetes: correlation between antiviral activity, cytotoxicity and antibacterial activity. (Studies on antiviral and antitumor antibiotics. XI).


Control of carbamyl phosphate synthesis in Salmonella typhimurium.


Cold sensitivity and other phenotypes resulting from mutation in pyrA gene.


The asparagine synthetase of Escherhic coli. I. Biosynthetic role of the enzyme, purification, and characterization of the reaction products.


The asparagine synthetase of Escherichia coli. II. Studies on mechanism.


Newark and the great influenza pandemic of 1918.


Possible interaction of luteinizing hormone-releasing factor with other hypothalamic releasing factors at the level of the adenohypophysis.


Registration of the myeloarchitecture of the human frontal lobe with an extinction method.


More on medicare and medicaid.

More on medicare and medicaid.


Radiofrequency thermal hypophysectomy. Technical note.


Medicare: boon to the aged or cruel hoax?


The "pocked" erythrocyte. Red-cell surface alterations in reticuloendothelial immaturity of the neonate.


Thomas William Francis Gann--Mayan explorer.


Development of endocrine function in the human placenta and fetus (second of two parts).


Rubbish in the red cell.

Rubbish in the red cell.


Involvement and leadership.

Involvement and leadership.


High-voltage electron microscopy.

High-voltage electron microscopy.


Earpiece dye-dilution technics for estimation of the left-to-right shunt in infants and children.


A simplified method for calculation of the leftto-right shunt from a single earpiece dye-dilution curve.


Tissue typing and transplantation.

Tissue typing and transplantation.


Bacteria and autoimmunity.

Bacteria and autoimmunity.


Chronic allogeneic disease as a model of autoimmunity and malignancy.


Immune complexes and autoimmune diseases.


Incorporation of lyophilized and radiosterilized perforated and unperforated bone grafts in dogs.


Comparison and analysis of the results of cytodiagnosis and frozen sections during operation.


Antitumor activity and other biologic properties of L-asparaginase (NSC-109229)-a review.


Clinical studies of n-hydroxyurethan (NSC-83629).


Effects of 5-fluorouracil (NSC-19893) in 389 patients with cancer.  Eastern Clinical Drug Evaluation Program.


Effects of 6-mercaptopurine (NSC-755) in 290 patients with advanced cancer. Eastern Clinical Drug Evaluation Program.


Effects of chlorambucil (NSC-3088) in 374 patients with advanced cancer. Eastern Clinical Drug Evualuation Program.


Effects of meturedepa (NSC-51325) in 233 patients with advanced cancer. Eastern Clinical Drug Evaluation Program.


A simple method for preparing meiotic chromosomes from mammalian testis.


Blood volume in psoriasis.

Blood volume in psoriasis.


New drug development.

New drug development.


Comparative controlled trial of rimactane in pulmonary tuberculosis.


Colour in subtraction angiography. Preliminary report.


Video tape recording in selective catheterization.


The Pi complex. A review of hyaluronate-protein complexes.


Henry Rose Carter, Jr.--epidemiologist--sanitarian.


Scanning electron microscopy of the skin using celluloid impressions.


In Rhodesia then. The daily graphic, Tuesday, 21st September, 1897. Hospital life in Matabeleland. I. Bulawayo.


Laboratory evaluation of antiepileptic drugs. Review of laboratory methods.


Note on plasma volume in aging rats.

Note on plasma volume in aging rats.


County Medical Society Directory.

County Medical Society Directory.


Principles of therapy in cerebral vascular disease.


Quantitative studies on the preservation of choline and ethanolamine phosphatides during tissue preparation for electron microscopy. I. Glutaraldehyde, osmium tetroxide, Araldite methods.


Quantitative studies on the preservation of choline and ethanolamine phosphatides during tissue preparation for electron microscopy. II. Other preparative methods.


Skin window studies of the effect of endotoxin upon the acute inflammatory response.


Formation and detection of RNA-DNA hybrid molecules in cytological preparations. A technique is described for forming molecular hybrids between RNA in solution and the DNA of intact cytological preparations. Cells in a conventional tissue squash are immobilized under a thin layer of agar. Next they are treated with alkali to denature the DNA and then incubated with tritium-labeled RNA. The hybrids are detected by autoradiography. The technique is illustrated by the hybridization of ribosomal RNA to the amplified ribosomal genes in oocytes of the toad Xenopus. A low level of gene amplification was also detected in premeiotic nuclei (oogonia).


Structure and function of E. coli ribosomes. 8. Cold-sensitive mutants defective in ribosome assembly. E. coli mutants that fail to grow at 20 degrees C were isolated, and three such mutants were studied in detail. Two of these are defective in 50S ribosome biosynthesis at low temperatures and accumulate 43S and 32S particles, respectively. The third mutant produces reduced amounts of 50S ribosomes and accumulates 21S particles at low temperatures. The 43S and 32S particles contain 23S RNA and appear to be precursors of 50S ribosomes. The 21S particles contain 16S RNA. The mutation that causes accumulation of 32S particles is linked to the spc locus. Isolation and characterization of coldsensitive mutants should provide a fruitful approach to the study of in vivo ribosomal assembly.


Recessive lethals: a new class of nonsense suppressors in Escherichia coli. Two new nonsense suppressors in Escherichia coli were found in partial diploids carrying F'14 and were shown to be on the episome. These suppressors can exist only in cells which also contain the su(-) allele, i.e., su(+)/su(-) heterozygotes. Presumably the mutations cause an alteration of an essential cellular component, the complete loss of which is lethal. Su7, an amber suppressor, has an efficiency of 76 per cent and su8, an ochre suppressor, an efficiency of 4 per cent.


Transient activation of RNA polymerase in Escherichia coli B after infection with bacteriophage T4. Sköld and Buchanan(1) have reported that there is a rapid loss of RNA polymerase activity in Escherichia coli B after infection with T4 bacteriophage. More recent studies on the mechanism of this inactivation have been made in this(2) and other laboratories.(3, 4) In this communication, we report the observation of a transient stimulation of RNA polymerase activity when measurement is made immediately after infection and when cells are ruptured by a gentle lysis procedure. The increase in activity is independent of the synthesis of protein. The activity in the extracts of infected cells is lost by treatment of the extract with antibody to E. coli RNA polymerase and is refractive to the inhibitory action of the antibiotic rifamycin. Hybridization experiments indicate that an RNA transcribed almost exclusively from a T4 DNA template is the product of incubation of extracts of infected cells with a reaction mixture containing an exogenous primer (salmon sperm DNA). These findings are consistent with the hypothesis that one of the first steps in phage infection is the formation of a transcription complex containing T4 DNA and E. coli RNA polymerase.


The role of selection in the host-mediated assay for mutagenicity.


Studies on repression of the histidine operon. II. The role of the first enzyme in control of the histidine system. Recent studies on repression of the enzymes for histidine bio-synthesis in Salmonella typhimurium demonstrated that the kinetic pattern in which the enzymes become repressed is influenced by the state of the feedback-sensitive site of the first enzyme of the pathway (Kovach et al., J. Bacteriol., 97, 1283 (1969)). In the present study we demonstrate that under certain conditions alteration of the feedback-sensitive site of the first enzyme prevents repression of the histidine operon. We conclude that the first enzyme plays a previously unrecognized role in regulation of the histidine system.


Host specificity of DNA produced by Escherichia coli. XI. In vitro modification of phage fd replicative form. An enzymatic activity, having the properties expected of a B-specific host-controlled modification enzyme, has been purified from an extract of Escherichia coli strain B. This activity renders the unmodified replicative form of phage fd resistant to B-specific restriction and is only present in strains carrying intact genes for type B modification. In phosphate buffer, the enzyme acts optimally at pH 6 and is dependent upon a single cofactor, S-adenosylmethionine.


The roentgen evaluation of bacteriuria associated with pregnancy.


Bile-blood fistula: a complication of percutaneous transhepatic cholangiography.


Transfer of antibiotic resistance from animal and human strains of Escherichia coli to resident E. coli in the alimentary tract of man.


Combined glaucoma and cataract operation.


Partial somatotrophin insufficiency in Cushing's syndrome.


The effect of homologous antilymphocytic serum on rabbit corneal heterografts.


High dose trifluoperazine therapy in chronic schizophrenia.


A psychiatric study of the life and work of Dorothea Dix.


The role of grief and fear in the death of kidney transplant patients.


Psychiatric complications of heart transplants.


Variation in the N-terminal sequence of heavy chains of immunoglobulin G from rabbits of different allotype. The sequences of the N-terminal peptides prepared by Pronase digestion of the heavy chain of rabbit immunoglobulin G of allotype Aa1, Aa2 and Aa3 were determined and were shown to be related to the allotype. An N-terminal fragment of about 34 residues was also prepared from the allotype heavy chains, by cleavage with cyanogen bromide; the yield varied with the allotype. The sequences of the cyanogen bromide fragments from the Aa1 and Aa3 heavy chains contain allotype-related variations similar to those found in the N-terminal Pronase peptides, and these sequences are thought to be representative of the whole heavy-chain populations. There is about 60% homology between the two sequences, and superimposed on the differences between them there are a number of positions within each sequence at which at least two amino acids are present.


The mycology of the clinically normal scalp.


Microvascular pattern and blood stasis in skin disease.


A Spanish bill of health (1790).

A Spanish bill of health (1790).


Gaubius on hypotheses in pathology.

Gaubius on hypotheses in pathology.


Alfred Goodman Levy--Bulawayo's first doctor.


Bantu siderosis--a review.

Bantu siderosis--a review.


The development of automated systems for clinical and research use.


Recent advances in the diagnosis of the diseases of the thyroid.


The use of white cells as a source of diagnostic material for lipid storage diseases.


The intradermal cancer test (ICT).

The intradermal cancer test (ICT).


Prolongation of life: role of free radical reactions in aging.


Ultrastructural studies of radiation-induced chromosome damage. The fine structure of radiation-induced chromosomal aberrations in Potorous tridactylis (rat kangaroo) cells was examined in situ by electron microscopy. The observations on the structure of terminal deletions (acentric fragments), anaphase bridges and "gaps," sidearm bridges, and specialized regions, such as the nucleolus organizer, are discussed in detail. Conclusions based on these observations are the following: (a) damage is physically expressed only at anaphase; (b) a gap region is composed of two subunits, each of which is about 800-1000 A in diameter and may correspond to a half-chromatid structure; (c) the ends of acentric fragments are structurally similar to normal chromosome ends, except where the break occurs in a specific region such as the secondary constriction; (d) at metaphase the fragment and the main portion of the chromosome move as a single unit to the equator, and the two units are disconnected only at the onset of anaphase; (e) sidearm bridges appear to be exchanges, involving a subchromatid unit. The interpretation of this evidence is consistent with the hypothesis that the chromosome is a multistranded structure.


Oral contraceptives.

Oral contraceptives.


Characterization of a 43 s ribonucleoprotein component of a mutant of Escherichia coli.


Beta granule formation in isolated islets of langerhans: a study by electron microscopic radioautography. The distribution of radioautographic grains over organelles within the beta cells of rat islets of Langerhans was investigated at various times after pulse labeling of the isolated islets with tritium-labeled amino acids. Ten minutes after the start of labeling most of the grains were situated over the endoplasmic reticulum and cytoplasm; by contrast, 60 min from the start of labeling the majority of the grains were associated with the beta granules. At 20, 30, and 45 minutes after pulse labeling the proportion of grains associated with the Golgi complex was increased two- to three-fold over the 10- or 60-minute values. The distribution of radioautographic grains over granules in the intact cells did not suggest that the electron-lucent type of secretory granules were precursors of the electron-opaque granules. Furthermore, studies of the pattern of grains over granules isolated by centrifugation 60 min after pulse labeling showed no preferential labeling of the electron-lucent type of granule. It is concluded that labeled amino acids are incorporated initially in the endoplasmic reticulum, and that the label subsequently appears in the beta granules. The Golgi complex participates either in the formation of the beta granule or in the translocation of the granule through the cytoplasm of the cell.


Immunological identification of a ribosomal protein on a sub-ribosomal particle.


Gastro-intestinal side effects and psychotropic drugs.


The antibacterial activity of cleaning compounds used in South Australian Hospitals.


Current models for the structure of biological membranes.


A history of L. Richardson Memorial Hospital.


A simple surgical treatment of intraoral fibrous scars utilizing a skin graft. Report of a case.


Bernhard Von Langenbeck. His life and legacy.


Present status of retropharyngeal implantation for velopharyngeal insufficiency.


Current progress in the management of cancer. A collective review.


Surgical treatment of chronic radiation injuries of axilla and shoulder.


Photoplethysmography as an aid in determining the viability of pedicle flaps.


Rhinoplastic operation, performed with success at the Hospital St. Eloi de Montpellier by Professor Jacques-Mathieu Delpech. (Reprinted from Lancet, 4: 123, July 24, 1824).


The classical reprint: rhinoplasty.

The classical reprint: rhinoplasty.


Nasolabial flap for losses of the nasal ala and columella. Case report.


Nasolabial flap for alar defect. Case report.


Renal disease and diabetes. Introduction.


Malabsorption syndrome.

Malabsorption syndrome.


Mr. Baragwanath.

Mr. Baragwanath.


Effects of serum from cholecystectomized persons on HeLa-cells.


Pancreatic islet morphology in diabetic Chinese hamsters. A light and electron microscopic study.


Protein A from Staphylococcus aureus. VII. Physicochemical and immunological characterization.


Studies on the complement fixation test with Mycoplasma pneumoniae antigen. 4. Demonstration of not cell-bound antigen in broth culture.


A new E. coli serotype O149:K9 (B), K88ac (L): H10 isolated from diseased swine.


The Albert H. Ketcham Memorial Award. Presentation to Lowrie J. Porter and W. M. Krogman by Wilbur J. Prezzano, president of the American Board of Orthodontics.


Renal transplantation.

Renal transplantation.