Clinical evaluation of the urinary sediment after renal allotransplantation. The detection of lymphoid cells by routine examination of the urine after renal allotransplantation has proved to be a useful early indication of rejection. In a study of 36 rejection episodes, 20 (56%) were associated with a significant number of lymphocytes in the urine. The incidence was much higher when rejection occurred during the first month after operation (76%); lymphocytes were rarely found when rejection occurred after three months. The appearance of lymphocytes in the urine was of particular value for detecting rejection in patients with prolonged oliguria after transplantation.


Carl Wilhelm Scheele (1742-1786) Swedish apothecary.


A clinical trial of disodium cromoglycate in asthma.


Cadwallader Colden (1688-1776) Lieutenant-Governor of New York Colony.


Temperature changes following iontophoretic injection of acetylcholine into the rostral hypothalamus of the rat.


Distribution, excretion and metabolism of trifluoperazine methiodide-C14 in rats.


Carbohydrate homeostasis. I.

Carbohydrate homeostasis. I.


Time of appearance of human placental lactogen in the embryo.


Franz Goldmann.

Franz Goldmann.


Cure of dysgerminoma with widespread metastases appearing after renal transplantation.


A review of developments in nutrition as it is related to fertility in cattle: 1964-9.


The management of renal failure.

The management of renal failure.


Eulogy for E. Richard Weinerman and Shirley Basch Weinerman.


Identification difficulties with Shigella dysenteriae, serotype 1.


Thermal stability of lactic dehydrogenase from human tissues.


Computer control in the clinical laboratory.


In memoriam. James Hallett Peers, M.D. 1905-1966.


Further use of statens in the recording, reporting, analysis, and retrieval of automated computerized laboratory and clinical data.


Pathogenesis of cardiac lesions in specific-pathogen-free chicken embryos infected with mycoplasma synoviae.


Automatic systems for detecting cystine and cystinyl peptides during column chromatography.


Sequence analysis of complex protein mixtures by isotope dilution and mass spectrometry.


Subunits, composition, and related properties of succinyl coenzyme A synthetase.


Excision repair.

Excision repair.


The interconversion of lethal and sub-lethal damage in UV-irradiated Escherichia coli B-R.


A single-plate screening test for potential radiosensitisers of hypoxic bacteria.


Radioisotopic evaluation in oncology.

Radioisotopic evaluation in oncology.


Chronic effects of nicotine on rat gastric secretion.


Ascending neurons of the spinal cord activated by cold.


Maturation of human ovarian follicular oocytes in vitro.


Occurrence of a characteristic nucleolar structure in pachytene cells.


Calf thymus fractions: enhancement and suppression of immunocompetent cells.


The environmental crisis. Commentary on the 1970 European Conservation Year.


Failure of diethylcarbamazine citrate (Hetrazan) in the treatment of asthma.


A study of the occurrence of atopic traits in patients with keratoconus.


Substrate specificity of the nicking enzymes isolated from phage T4-infected Escherichia coli.


Amino acid sequence of the coat protein of the RNA phage ZR.


Beethoven's deafness.

Beethoven's deafness.


Henri-Louis Roger (1809-1891): Roger's Disease.


Needle aspiration biopsy technique in pulmonary disease. Application to therapy.


Differences in fibre size of histologically processed pre- and post-rigor mouse skeletal muscle.


Long-term storage of acetic acid bacteria by means of lyophilization.


Active chronic hepatitis.

Active chronic hepatitis.


Optic disc typing--a technique to improve fundus examination.


Kenneth Berkeley Stoddard 1904-1970.

Kenneth Berkeley Stoddard 1904-1970.


A selected public health bibliography with annotations.


Childhood bullous dermatitis herpetiformis. Negative immunofluorescent tests.


Senear-Usher syndrome (pemphigus erythematosus). Immunofluorescent studies in a patient.


Favus again mistaken for leprosy in the Renaissance.


Sedimentation of ribosomal ribonucleic acid from ribonuclease treated and untreated ribosomes.


Development of methodologic tools for planning and managing library services. IV. Bibliography of studies selected for methods and data useful to biomedical libraries. This selective bibliography is intended to serve as a guide to empirical studies reporting data and methods that can be used by medical librarians to assess their own efforts objectively and to improve the effectiveness and efficiency of services they offer. The decision rules that governed selection of items for the bibliography are specified in detail. A total of 178 items published between 1915 and mid-1968 met the selection criteria. The list of items is supplemented by a keyword index derived from titles. Half the items are journal articles; a third of these articles appeared in the Bulletin, and most of the remainder in thirteen other library and information science journals. Most of the non-journal items are technical reports issued by the organization that conducted or sponsored the work. The characteristics of this literature suggest that few medical libraries, unless they are part of a university system that includes the collection of a library or information science school, are likely to have quick access to the literature base needed to support a comprehensive program of self-evaluation studies and the continuing education of their own staff. Regional medical libraries might well undertake to ensure both ready access to, and awareness of, literature on the scientific aspects of librarianship.


Donald Washburn, D.D.S. President, Medical Library Association, 1970-71.


Reticulum cell sarcoma of the breast. Case report and a review of the Japanese literature.


Backward associations in paired-associate learning of retardates and normal children.


Determinants of part-whole preception in children.


Measurement of tritium in neat plasma and urine with a toluene/triton X-100/hyamine 10-X scintillant.


Early detection of injury after nitrogen mustard (HN2) therapy: the radioiron disappearance rate as a dosimeter.


Successful treatment of acromegaly: metabolic and clinical studies in 145 patients.


Histology and ultrastructure of untreated forty-eight-hour canine renal transplants.


Effects of temperature, photoperiod, and hibernation on the testes of golden hamsters.


The influence of early extirpation of the otocysts on development of behavior of the chick.


Knobby, a semilethal mutation resulting in defective down in the turkey.


Cells involved in the immune response. 18. Potential hemolysin-forming cells in the sacculus rotundus of the normal adult rabbit.


Primary malignant lymphomas of the digestive tract. Study of 32 cases.


Ultraviolet action spectra for mutation in Escherichia coli.


Studies on the possible product of a bacterial mutator gene.


Enhanced efficiency of excision repair of non-replicated UV-damaged E. coli DNA.


Evidence for the control of respiration by DNA in ultraviolet-irradiated Escherichia coli B-r cells.


Influence of DNA replication on the X-ray sensitivity of bacteria.


Gene order of RNA bacteriophage M 12.

Gene order of RNA bacteriophage M 12.


Neural degeneration and regeneration in human renal transplants.


A controlled family study of live, attenuated rubella-virus vaccine.


Carbohydrate homeostasis.

Carbohydrate homeostasis.


Renal nerves: are they essential?

Renal nerves: are they essential?


Karl Jaspers 23. 2. 1883--26. 2. 1969.

Karl Jaspers 23. 2. 1883--26. 2. 1969.


McArdle's syndrome.

McArdle's syndrome.


The central flow tilting disc valve prosthesis (Björk-Shiley) for mitral valve replacement.


Gösta Jönsson.

Gösta Jönsson.


A review of cardiac transplantation in man. Introductory lecture at the Meeting of the Scandinavian Society of Thoracic Surgery, Copenhagen, August 28-30, 1969.


Antifibrinolytic therapy with Cyklokapron in connection with prostatectomy. A double blind study.


Mannitol infusion in regular haemodialysis treatment for chronic renal insufficiency.


Testicular blood flow in man measured with xenon-133.


Use of antilymphocyte globulin prepared by precipitation with Rivanol and reduced dose of azathioprine in allogenic renal transplantation in the dog.


Renal blood flow in dogs studied by means of a dye-dilution technique.


Diploid azaguanine-resistant mutants of cultured human fibroblasts.


The use of drugs in the management of strabismus.


Genetic recombination in bacteriophage f1: transfer of parental DNA to the recombinant.


Regulation of cessation of early enzyme synthesis in bacteriophage T4 infected Escherichia coli: separate mechanisms for different enzymes.


Specialized transduction of galactose by lambda phage from a deletion lysogen.


Stages in phage R17 infection.

Stages in phage R17 infection.


Prophage excision: on the status of host chromosome after induction.


Immunofluorescent localization of double-stranded RNA in reovirus-infected cells.


Mu-1 bacteriophage DNA.

Mu-1 bacteriophage DNA.


Molecular size and charge relationships of the soluble complement-fixing antigens of dengue viruses.


Thermal induction of bacteriophage Plb.

Thermal induction of bacteriophage Plb.


Intravital observations on the microvascular anatomy and microcirculation of the tendon.


Dendritic (herpetic) keratitis. II. Follow-up examination of corneal opacity. (Opacity, vascularisation, Hudson-Stähli's line, sequelae of iritis).


Demonstration of VD-virus by the fluorescent antibody technique in tissues of cattle affected with bovine viral diarrhea (mucosal disease).


Total and regional cerebral blood flow in unanesthetized dogs.


Mendelian genetics and selction for quantitative traits in poultry: results and perspectives.


Incidence of diagnostic x-ray in orbital bones in exophthalmos in U.A.R.


Lamellar and penetrating pectangular autogenous keratoplasty.


Immunological aspects in keratoplasty. A forward to immunity in keratoplasty.


Surgery of partial lamellar keratoplasty complicated by a tiny perforation of recepient corneal bed.


Immunological aspects in keratoplasty. Influence of the method of preparation of corneal antigen on its antigenicity.


Immunological aspects in keratoplasty. Immunological reactions occurring in the eyes of rabbits previously sensitized by corneal antigen.


Estradiol and the neuroendocrine control of LH release in vitro.


Release of LH-releasing factor (LRF) into the peripheral circulation of hypophysectomized rats by dopamine and its blockade by estradiol.


Localization of LH-releasing factor in the hypothalamus and neurohypophysis as determined by an in vitro method.


Comparison of single-breath and indicator-dilution measurement of cardiac output.


Gastric pepsin determinations: an automated method.


Measurement of intrarenal transit time and hemoglobin saturation by fiberoptics.


Application of impedance cardiography to study of postural stress.


Absence of antigenicity in freeze-dried skin allografts.


Continuous infusion indicator-dilution measurement of blood flow in the dog forelimb.


Cardiac output during submaximal and maximal treadmill and bicycle exercise.


Computer analysis of myocardial tension and pressure variations in atrial fibrillation.


A review of basic researches on the cryopreservation of red blood cells.


A new test of the reserve capacity of the pituitary to secrete thyrotrophic hormone (TSH-reserve test).


Effect of porcine calcitonin on hypercalcemia in man.


John Hunter's private press.

John Hunter's private press.


Race, mortality, and life insurance: negro vital statistics in the late nineteenth century.


The doctoral thesis of Engelbert Kaempfer on tropical diseases, oriental medicine, and exotic natural phenomena.


Four monopolies and the surgeons of London and Edinburgh.


The physicians of Syria during the reign of Salăh al-Dĭn 570-589 A.H. 1174-1193 A.D.


Edward Foote's Medical Common Sense: an early American comment on birth control.


The lying-in room.

The lying-in room.


Sir Thomas Browne, Thomas le Gros, and the first edition of Pseudodoxia Epidemica, 1646.


P. Earle Williams, Vice President 1969-70.


Editorial. The first fifty years.

Editorial. The first fifty years.


The American College of Dentists. Its origin and structure.


Fifty yeats of public and professional relationships and still looking ahead.


Dental journalism and The American College of Dentists.


The American College of Dentists: a critique.


The struggle against doping and its history.


Lymphosarcoma with Bence Jones proteinuria (type K) and IgG serum M-component (type L): a bitypic gammopathy.


Heart transplant: the whole world watches.


Group A streptococcal puerperal infection. Report of an epidemic.


Chromosomes of normal and neoplastic ovarian tissue.


Etiology and management of oblique lie.

Etiology and management of oblique lie.


Atresia of the vagina.

Atresia of the vagina.


Amnion nodosum and hypoplastic cystic kidneys. An electron microscopic and microdissection study.


Communicating in writing. II.

Communicating in writing. II.


Catalase: Physical and chemical properties, mechanism of catalysis, and physiological role.


Mitochondrial nucleic acids and their relation to the biogenesis of mitochondria.


Effects of ingestion of disproportionate amounts of amino acids.


Clinical experience with amicillin and probenecid in the management of treponeme-associated uveitis.


A foreword.

A foreword.


The biochemistry of B12-mediated reactions in man.


Interrelationships of vitamin B12 and folate in man.


Absorption and malabsorption of vitamin B 12.


The absorption and malabsorption of folic acid and its polyglutamates.


Inherited defects of B12 metabolism.

Inherited defects of B12 metabolism.


Drugs, toxins and dietary amino acids affecting vitamin B12 or folic acid absorption or utilization.


The nephrotic syndrome associated with secondary syphilis. An immune deposit disease.


Immunofluorescent demonstration of myoglobin in the kidney. Case report and review of forty-three cases of myoglobinemia and myoglobinuria identified immunologically.


Differential diagnosis and management of the patient with megaloblastic anemia.


Changing orthoptic practice.

Changing orthoptic practice.


Aniseikonia and space perception--after 50 years.


William Lemuel Benedict, MD. February 13, 1885 - February 18, 1969.


Ralph Bodkin Kelly, O.B.E., D.V.Sc.

Ralph Bodkin Kelly, O.B.E., D.V.Sc.


Depression of weak allograft immunity in the mouse by neonatal or adult exposure to urethan.


Inhibitory effect of L-asparaginase in lymphocyte transformation induced by phytohemagglutinin.


Effect of beta-propiolactone on bacteriophages and Salmonella typhimurium.


The effect of the antitumor agent 1,1-diphenyl-2-propynyl cyclohexylcarbamate on the immune response of mice to sheep red blood cells.


Enhancement by estrogens of adenovirus or spontaneous transformation of hamster cell cultures.


Clinically deceptive massive pulmonary embolism.


The role of the bronchologist in respiratory care. Remarks on indications and technics for mechanical ventilation in lung disease.


Identification of two cell populations required for mouse immunocompetence.


A tribute to Vincent J. Derbes, M.D.

A tribute to Vincent J. Derbes, M.D.


Ontogeny of cell populations required for immunocompetence.


Immunoglobulin classes of antibody-forming cells in mice. IV. The incorporation of tritiated thymidine into IgM and gamma 1 plaque-forming cells during the primary immune response.


Laboratory diagnosis of syphilis.

Laboratory diagnosis of syphilis.


The immune response of mice to Serratia marcescens LPS or intact bacteria.


Genetic control of the antibody response to simple haptens in congenic strains of mice.


Relationship of acetyl transferase activity to antinuclear antibodies and toxic symptoms in hypertensive patients treated with hydralazine.


Serial perfusion and ventilation studies following reimplantation of the lung using xenon 133, pulmonary angiography, and bronchography.


Patterns of 35S-thiamine hydrochloride absorption in the malnourished alcoholic patient.


Experience with the Wada-Cutter valve prosthesis in the aortic area. One-year follow-up.


Recent experience with thalidomide in Hansen's Disease.


Physiological changes in the postnuclear cap region of mammalian spermatozoa: a necessary preliminary to the membrane fusion between sperm and egg cells.


Ultrastructural changes in the hamster sperm head during fertilization.


Tubular aggregates in type II muscle fibers: ultrastructural and histochemical correlation.


Location of the neural structures triggering ovulation in the rat.


The effect of presets on creative problem solving.


The immunodepressive activity of N-diazoacetylglycine amide in some transplantation systems.


Histoincompatibility associated with the Z chromosome in chickens.


Effect of 24-hr perfusion with yeast RNA on canine kidney allografts.


Strain-specific renal toxicity of heterologous antilymphocyte gamma-globulin in mice.


Renal transplantation in the inbred rat. XV. An assay study of three immunosuppressive drugs.


Immunogenic properties of papain-solubilized H-2 alloantigens.


Survival of guinea pig skin grafts in the brains of rats under treatment with antilymphocytic serum.


Allergic adrenalitis in adrenal isografts.


Compliance with medical regimens: a review of the literature.


Physiology of mast cells. I. Behaviour of mast cells in tissue culture (microcinematographic observations).


Persistence of malaria antibody in Tobago, West Indies, following eradication, as measured by the indirect hemagglutination test.


Action of interferon and its inducers aginst nonviral infectious agents.


The development and characteristics of dental porcelain.


Effect of a film of salivary origin on enamel solubility reduction by stannous fluoride.


Evaluation of sedatives in dental practice. Preliminary report.


Comparison of the performance of materials for spring members in dental appliances, using the theory of simple bending.


Sulcoplasty and the use of skin grafts in preprosthetic surgery.


Mechanism of elevated innominate artery pressures in supravalvular aortic stenosis.


General hospital psychiatry: transatlantic impressions.


Understanding the schizophrenic.

Understanding the schizophrenic.


Free full-thickness skin grafts from the palm to cover defects of the fingers.


Separation of massive avascular osteocartilaginous fragment of femoral condyle following renal transplantation. Report of two cases.


Annual educational meeting in honor of Ward Darley, M.D. Comprehensive health care: introduction.


Current titles in periodontology.

Current titles in periodontology.


Oral clinical functionality of enzyme AP used as a mouthwash.


Tom E. Nesbitt, M.D.: 82nd president--Tennessee Medical Association.


Prolongation of QRS duration in acute myocardial infarction.


Disorders of red cell membrane: history and perspectives.


Ultrastructure of red cell membranes.

Ultrastructure of red cell membranes.


Determination of the corticosterone content of rat organs and tissues by the method of 14C-acetic acid anhydride derivatives.


The lymphatic system as contributor of plasminogen activator to the blood.


Chronic venous insufficiency treated with hydroxyethylrutosides (HR).


Changes of the apparent 3HOH, 82Br, 125I human albumin and 51Cr red blood cell dilution volumes before, during and after operation in human subjects.


Urological complications in 216 human recipients of renal transplants.


Tertiary hyperparathyroidism following successful renal allografting.


Serratia marcescens sepsis in a combat zone.


Orthotopic and heterotopic liver homografts in man.


Methotrexate and the ultraviolet light inflammation in the guinea pig.


Action of insulin on isolated fat cells.


Hemodynamic aspects of the pulmonary circulation during retrograde perfusion.


Mechanisms of immunosuppression by heterologous anti-lymphocyte serum (ALS) and plasma (ALP).


Gentamicin. Clinical and pharmacological studies.


Combined application of quinidine and propranolol for the restoration and maintenance of sinus rhythm in supraventricular arrhythmias.


Modification of penicillin distribution and elimination by probenecid.


Preparation of contact negative from large histological sections.


Visible spectra of steroids treated with the EMA reagent.


The dependence of hippocampal RNA levels on the frequency of afferent stimulation.


The diagnostic significance of the intrauterine pressure. I.


Walter Kempler, M.D.

Walter Kempler, M.D.


Samuel J. Fomon M.D.

Samuel J. Fomon M.D.


Observations on human ova and the fertilization process.


In memoriam D.G. McKerracher 1909-1970.

In memoriam D.G. McKerracher 1909-1970.


Raymond Walter Waggoner, Sr., M.D. ninety-eighth President, 1969-1970.


A history of the concept of neurosis, with a memoir of William Cullen.


Relationship between electroencephalographic and sociometric variables among psychiatric patients.


The epiphyseal complex in fish and reptiles.


Endocrinology of the amphibian pineal.

Endocrinology of the amphibian pineal.


Structure and alleged functions of avian pineals.


Endocrine effects of the mammalian pineal.


Reproductive effects of the mammalian pineal.


Cells and organizers.

Cells and organizers.


The search for better anesthetic agents: clinical investigation of ethrane.


Cardiac arrest during IPPV in a newborn with tracheoesophageal fistula.


New data on the toxic and radioprotective effect of cysteine on Escherichia coli 15 T- cells.


Histopathological examinations of preserved and resustitated dog hearts (storage of hearts for transplantation).


Study of increased resistance to ultraviolet radiation mechanism after omission of energy source from medium in the strain Escherichia coli 15 T-U-his-.


Air pollution vs. healing. II. Air pollution, healing, and civilization.


Carbon dioxide therapy, healing, and air pollution. A more effective rapid coma technic for psychiatric disorders.


Antidiuresis in man following lysergic acid diethylamide and mescaline.


A comparison of haloperidol and thioridazine HC1 in chronic treatment-resistant schizophrenics.


History of standardization in mechanical vibration and shock.


The lenis-fortis opposition: its physiological parameters.


H. Sicher to receive Gold Medal Award.

H. Sicher to receive Gold Medal Award.


I. Impact during the industrial revolution and now. The urban avalanche and child nutrition.


Iron absorption in the Bantu.

Iron absorption in the Bantu.


Use of autogenous bone from the symphysis for treatment of delayed union of the mandible: report of case.


Cortisol responses to diazoxide in man and their possible relationship to effective blood volume.


Hand-Schüller-Christian Disease: two case reports.


Nonsuppressible insulin-like activity: immunochemical and physicochemical evidence against its pancreatic origin.


Factors related to tooth number, eruption time, and tooth position in cleft palate individuals.


Bacteriophage lambda; abortive infection of bacteria lysogenic for phage P2. The efficiency of plating of wild-type lambda on a host lysogenic for P2 is less than 10(-6), and only a small number of infected cells produce progeny phage. Lambda can adsorb and inject its DNA normally in such cells; the DNA can circularize and is not nicked or degraded, but replication is severely impaired. Mutants of P2, which as prophages no longer interfere with lambda, have been isolated and found to be recessive to wild type, implying that P2 prophage codes for a diffusible product involved in lambda interference. The P2 gene product responsible for preventing lambda growth also kills recombination-deficient bacteria of the recB and recC classes under conditions where P2 does not normally kill the host. Mutants of lambda that are resistant to interference are recessive to wild-type lambda. Thus lambda actively participates in its own interference. The lambda-mutants that are resistant to interference are unable to synthesize at least two nonessential proteins. In addition, they are unable to grow on recombination-deficient bacteria of the recA class, but they can grow on recA recB double mutants.


Replicative form II DNA of phi X174; resistance to exonucleolytic cleavage by E. coli DNA polymerase. The progeny replicative form II DNA isolated from E. coli cells infected with varphiX174 is resistant to the exonucleolytic activity associated with E. coli DNA polymerase. A limited endonucleolytic cleavage with micrococcal endonuclease renders the replicative form II molecule susceptible to the exonucleolytic activity associated with the E. coli DNA polymerase. These experiments suggest that the resistance of the replicative form II DNA to the exonucleolytic activity is a result of the structure of these molecules at their 5'-terminus.


Use of dimethyl suberimidate, a cross-linking reagent, in studying the subunit structure of oligomeric proteins. Amidination of aldolase, glyceraldehyde-3-phosphate dehydrogenase, tryptophan synthetase B protein, L-arabinose isomerase, and the catalytic subunit of E. coli aspartate transcarbamylase with the bifunctional reagent dimethyl suberimidate produces cross-linked proteins, with reaction predominating within oligomers. Disc electrophoresis of a modified protein on polyacrylamide gel in the presence of sodium dodecyl sulfate resolves a set of species with molecular weights equal to integral multiples of the protomer molecular weight. For oligomers composed of identical protomers, the number of principal species observed is identical to the number of protomers in the oligomer. Application of the method to two proteins composed of dissimilar protomers, native aspartate transcarbamylase and tryptophan synthetase alpha(2)beta(2) complex of E. coli, revealed differences in the reactivities of the different kinds of protomer within each oligomer.


Initiation and release of RNA by DNA-dependent RNA polymerase. During in vitro transcription of T4 DNA by E. coli RNA polymerase, chain initiation stops coincidentally with synthesis at low ionic strength (0.11) with an average of one RNA chain initiated per 24S polymerase molecule. At high ionic strength (0.37), initiation as well as synthesis continues for several hours, with an average of four chains initiated per enzyme molecule in two hours. The RNA product is released from the T4 DNA template at both low and high ionic strength. At high ionic strength, however, RNA polymerase can repeatedly initiate, synthesize, and release RNA from the synthesis complex in vitro. Under both conditions, the synthesized RNA sediments at 25-44 S, has a number average chain length of 5000 to 7500 nucleotides, and a weight average chain length of 11,500 nucleotides.


Specific separation of cells on affinity columns. Cell filtration is performed on affinity columns prepared by the chemical modification of large polyacrylamide beads. Affinity columns selectively bind cells that produce antihapten antibodies of corresponding specificity, while cells without the specific antihapten sites pass through freely and are recovered quantitatively in the eluate. The binding of the antihapten specific cells is inhibited by hapten. Such cell filtration affinity columns may be useful in specifically purifying cell populations with characteristic membrane-receptor proteins.


A T4-induced endonuclease which attacks T4 DNA. A DNA endonucleolytic activity that introduces single-stranded breaks into native T4 DNA is found in T4-infected E. coli. This endonuclease attacks other double-stranded, but not single-stranded, DNA. Experiments with ultraviolet-irradiated phages and amber mutants indicate that the enzyme is made beginning about five minutes after infection at 37 degrees C.


Catabolite-insensitive revertants of lac promoter mutants. The maximum rate of expression of the lac operon is severely reduced in lac promoter mutants. Revertants of these mutations which produce higher levels of enzyme were isolated. Some of these revertants had lost sensitivity to catabolite repression and transient repression. The mutations responsible for these losses took place at sites very close to the original promoter mutations. From these results we conclude that the promoter itself is the target site for both catabolite and transient repression of the lac operon.


Segregation of functional sex factor into minicells. The segregation of a bacterial plasmid, the sex factor F', has been investigated in a cell-division mutant of Escherichia coli which produces small anucleate cells (minicells). Significant amounts of isotopically labeled DNA segregate into minicells dependent upon the presence of F'. Minicells containing F'Gal or F'(lambda) are shown to donate the plasmid in conjugation. These results demonstrate that the sex factor may be dissociated from the bacterial chromosome and that this separation does not prevent its subsequent transfer.


Nystagmus computation by analog technics. SAM-TR-70-7.


Ernest Witebsky, 1901-1969.

Ernest Witebsky, 1901-1969.


Genetic expression in RNA phages.

Genetic expression in RNA phages.


The RNA-overmethylation in brain tumours. Data and hypothesis.


The effect of skin allograft size on survival time following transplantation between mice differing at the H-2 locus.


Ernest Baldwin, 1909-1969.

Ernest Baldwin, 1909-1969.


Reliability of cardiac output measured by thermodilution method in small animals.


An anatomical review of the corpus callosum.


Clinico-pharmacological investigations with chlorpropamide in diabetes insipidus associated with diabetes mellitus.


Pre-endoscopic medication: parenteral diazepam used adjunctively.


Association of ulcer with chronic pulmonary disease.


Double-blind study of endoscopy premedications.


Trial of healing as a diagnostic technic for gastric ulcer.


Salmonella survival during spray drying and subsequent handling of skimmilk powder. I. Salmonella enumeration.


Salmonella survival during spray drying and subsequent handling of skimmilk powder. II. Effects of drying conditions.


Pituitary ovarian relationships in the cow.


Compartmentation of citric acid cycle metabolism in brain: labelling of glutamate, glutamine, aspartate and gaba by several radioactive tracer metabolites.


Irradiation and 5-fluorouracil as adjuvants in the management of invasive bladder carcinoma. A cooperative group report after 4 years.


Renal abscess.

Renal abscess.


Reconstruction of the penis.

Reconstruction of the penis.


Scintillation camera renography in the identification of an uncommon renal transplant complication.


Pancreatico-duodenal and renal allotransplantation in juvenile onset, insulin dependent, diabetes mellitus with terminal nephropathy.


Dermatitis due to tear gases (lacrimators).


Malignant lymphoma of the larynx.

Malignant lymphoma of the larynx.


Possible sites at which 5-hydroxytryptamine may antagonize in vivo neuromuscular paralysis.


A lawyer looks at heart transplants.

A lawyer looks at heart transplants.


The mechanism of photosynthesis and the structure of the chloroplast.


The evolution of bioenergetic processes.


Ionic channels in nerve membranes.

Ionic channels in nerve membranes.


The geometry of nucleic acids.

The geometry of nucleic acids.


Replicating DNA: structure of colicin factor E1. Replicating circular molecules of colicin factor E1 have been isolated from minicells produced by a colicinogenic derivative of Escherichia coli P678-54. The replicating structures observed in electron micrographs are circular molecules with two branched points. Several structures found among the replicating molecules are interpretable as replicating rolling circles.


Cystinosis: selective induction of vacuolation in fibroblasts by L-cysteine-D-penicillamine disulfide. In cultured fibroblasts from individuals with cystinosis vacuolation is induced by exposure to L-cysteine-D-penicillamine disulfide. Normal fibroblasts do not show vacuolation on such exposure. These observations provide direct evidence that cystinotic cells have deficient activity of a lysosomal system for disulfide metabolism or transport. Induction of vacuolation by the mixed disulfide in cystinotic but not in normal cells furnishes a histological marker for cystinotic fibroblasts.


Preservation of fine structures in yeast by fixation in a dimethyl sulfoxide-acrolein-glutaraldehyde solution.


Sticky wax infiltration in the preparation of sawed undecalcified bone sections.


Fallacies in child care. I. That "separation" is synonymous with "deprivation".


Cardiac pacing in acute myocardial infarction complicated by complete heart block.


Our surgical heritage.

Our surgical heritage.


In memoriam, Edwin H. Ellison, MD, 1918-1970.


Studies in intestinal healing. V. Bacterial population in intestinal anastomoses.


Benjamin P. Kissam, Naval surgeon in the war of 1812.


Relation of rheumatoid factor to intracellular inclusions.


The clinical physiology and pathology of the nasal airways and of their adjoining air-filled cavities.


In vivo interactions of antiglobulin factors.


The kinetics of cellular proliferation in normal and malignant tissues. X. Cell proliferation in the nose and adjoining cavities.


The effects of inhaled pollutants on nasal and pulmonary flow-resistance.


Sir Terence Cawthorne, F.R.C.S., 1902-1970.


The maintenance of function of the donor heart in the extracorporeal stage and during transplantation.


Multiple traumatic thoracic aneurysms after nonpenetrating chest injury.


Secondary syphilis misdiagnosed as lymphoma.


Recent advances in surgery of congenital heart disease.


Sir Henry Simpson Newland, 1873-1969.

Sir Henry Simpson Newland, 1873-1969.


Arterial injuries in civilian practice.

Arterial injuries in civilian practice.


A different cord lateralization procedure.


Adenocarcinoma of glandular stomach and duodenum in Wistar rats ingesting N-methyl-N'-nitro-N-nitrosoguanidine, histopathology and associated secretory changes.


Digitalis: two centuries later.

Digitalis: two centuries later.


The predictive value of skin allograft survival times during the development of urethan-induced lung adenomas in BALB-c mice.


Humanity and compassion in medicine (Ambroise Paré, Baron Dominique-Jean Larrey).


Businessmen and public health in the urban South during the nineteenth century: New Orleans, Memphis, and Atlanta.


Observations of the effects of kidney transplant on donors and recipients.


Ulcerative colitis: a contemporary overview.


Hypnotic properties of nitrazepam (Mogadon). (A comparative study of chlordiazepoxide, diazepam, nitrazepam, secobarbital and placebo in psychiatric patients).


Sir George Baker and the dry belly-ache.


Increase in the proportion of virus-producing cells and of several mutants in K12-lambda cultures exposed to ultraviolet light, triethylene melamine, or hydrogen peroxide.


On the chemical structure of antigens and antibodies bound to fluorescent dyes. I. Structure of the antihuman gamma-globulin of the goat bound to the fluorochrome lissamine rhodamine B.


Some diverse backgrounds for Curt Herbst's ideas about embryonic induction.


Effect of secretin and cholecystokinin on pentagastrin-stimulated gastric secretion in man.


Our new president: James A. Clifton, M.D.


Qualitative and quantitative relationships of the fecal flora in cirrhotic patients with portal systemic encephalopathy and following portacaval anastomosis.


Histocompatibility testing using leukocyte typing and mixed lymphocyte culture in kidney transplants.


Impotencia throughout the ages.

Impotencia throughout the ages.


Surgical drainage of cyst of the conus medullaris. Report of three cases.


History of skeletal traction in the treatment of cervical spine injuries.


Clinical presentation of reticulum-cell sarcoma, a disease with many faces.


An unusual zone surrounding colistin discs in sensitivity tests of Serratia marcescens.


Jerome Van Crowningshield Smith.

Jerome Van Crowningshield Smith.


Marihuana.

Marihuana.


Horsley and Clarke: a biographical medallion.


On the permeability of cestodes to colloids: an evaluation of the transmembranosis hypothesis.


In vitro studies of cellular responses to heat and vibration in cavity preparation.


Tribute to Frank Fremont-Smith, M.D.

Tribute to Frank Fremont-Smith, M.D.


The in vivo effect of folinic acid (citrovorum factor) on the potentiation of the antibacterial activity of sulfisoxazole by trimethoprim.


Alexander Forbes and his achievement in electrophysiology.


When the child's heart fails: Recognition, treatment, prognosis.


Fifty years as a woman doctor.

Fifty years as a woman doctor.


In memoriam.

In memoriam.


Professor Robert Koch in South Africa.

Professor Robert Koch in South Africa.


Leeching.

Leeching.


Mycoplasma culture media and subculturing techniques.


Isolation of mycoplasmas from human cord blood or serum: micro-anaerobic diphasic culture method.


William Hunter's romance.

William Hunter's romance.


Effect of cortisone on bronchopulmonary aspergillosis in mice exposed to spores of various Aspergillus species.


Renal failure in the elderly.

Renal failure in the elderly.


Penetrating wounds of the thoracic aorta and great arteries.


Dr. Everett S. Lain.

Dr. Everett S. Lain.


Electron microscopic observations on the renal caliceal wall in the rat.


Intercellular junctions in the developing neural retina of the chick embryo.


Organelle transport in constricted nerve fibers of the toad Bufo arenarum Hensel.


Effect of glibenclamide upon the isolated pancreatic islets of rat in vitro.


Safety evaluation of a quaternary ammonium sanitizer for turkey drinking water.


Protection against Marek's disease by vaccination with a herpesvirus of turkeys.


Allogeneic marrow engraftment following whole body irradiation in a patient with leukemia.


Relationship of erythropoietin effectiveness to the generative cycle of erythroid precursor cell.


Analytic review: disorders of phagocyte function.


Ernest Witebsky.

Ernest Witebsky.


Propanidid.

Propanidid.


Circulatory effects of pentazocine and pethidine during general anaesthesia with nitrous oxide, oxygen and halothane.


A comparison of the circulatory effects in man of the analgesics fentanyl, pentazocine and pethidine.


Bezitramide, an orally active analgesic. An investigation on pain following operations for lumbar disc protrusion (preliminary report).


The duration of action of bupivacaine, prilocaine and lignocaine.


Neuroleptanalgesia: a double-blind comparison of pentazocine and phenoperidine for neuroradiological investigations.


A history of nitrous oxide and oxygen anaesthesia. IVa. Further light on Hickman and his times.


Use of Farman entrainer and EMO vaporizer with IPPV.


Cutaneous responses to aspirin and its derivatives.


The plasma amino acid ratio as an indicator of the protein nutrition status: a review of recent work.


Model surveying.

Model surveying.


Ultraspeed instrumentation: atraumatic removal of tooth structure.


The interaction of emetine with DNA and its effect on the adsorption of certain bacteriophages.


Progress report: the carcinoid syndrome.


The kinetics of succinyl coenzyme A synthetase from Escherichia coli. A reaction with a covalent enzyme-substrate intermediate not exhibiting "ping-pong" kinetics.


The effects of urban air pollution on health.


Leakage of beta-galactosidase from phage-infected Escherichia coli: a re-evaluation.


A review of osteogenesis.

A review of osteogenesis.


Guides to medical writing.

Guides to medical writing.


Light and electron microscopy of cells infected with tobacco necrosis and satellite viruses.


A double-blind trial of bromelains as an adjunct to vaginal plastic repair operations.


Paul Goldhaber, D.D.S., Dean, Harvard School of Dental Medicine.


The ramus end-osseous implant.

The ramus end-osseous implant.


Points prosthetic 10. Post-insertion difficulties.


An old fogy speaks his mind.

An old fogy speaks his mind.


Membership list.

Membership list.


Use of the dansyl reaction in biochemical analysis.


I. Lawrence Kerr, D.D.S.

I. Lawrence Kerr, D.D.S.


Louse-borne relapsing fever.

Louse-borne relapsing fever.


South Africa. I.

South Africa. I.


Human genetics.

Human genetics.


Tissue response to pontic material and design.


Research at Temple Dental School--II.

Research at Temple Dental School--II.


Biomechanical relationships of the mandibular bilateral distal extension partial denture.


Leptospirosis.

Leptospirosis.


Beta-hemolytic streptococcal and concurrent infections in adults and children with respiratory disease, 1958 to 1969.


Locomotor and educational achievements of children with myelomeningocele.


A comparison of phenylbutazone and flufenamic acid in the treatment of acute gout.


A double-blind controlled trial of Rumalon in the treatment of painful osteoarthrosis of the hip.


The surgical treatment of pulmonary tuberculosis.


Urinary tract infection in patients with rheumatoid arthritis.


The role and the future of the hospital-based physician. 1.


Metastatic reticulum cell sarcoma presenting with hypercalcemia and terminating in diabetes insipidus-like syndrome.


Some properties of 2,2,6,6-tetra methyl-4-piperidinol N-oxyl, a sensitising agent for anoxic Escherichia coli.


Pedicled skin-flaps: the fallacy of the length: width ratio.


Intraperitoneal noxythiolin in faecal peritonitis.


Automobile accidents in children and surface covering of heel defect by thigh graft: a new method.


A survey of infantile gastroenteritis.

A survey of infantile gastroenteritis. In 1967 we admitted 339 cases of infantile gastroenteritis; one-third of these were dehydrated, and in this group the commonest biochemical abnormality found was hypernatraemia, sometimes with metabolic acidosis. A higher incidence of dehydration was found in the patients who had received oral glucose fluids before admission. EnteropathicEscherichia coliwere isolated from the faeces of 16% of the cases. Associated infections, especially of the respiratory tract, were common. Treatment was aimed at the restoration of fluid and electrolyte balance. Usually this was achieved with oral fluids, though intravenous fluids were used in the most severely dehydrated cases. Recovery was complete in 320 cases and a further 14 cases were discharged as carriers of enteropathicE. coli. There were five deaths (1.5%) in the series; three occurred immediately after admission.


Meningomyelocele and hydrocephalus.

Meningomyelocele and hydrocephalus.


In vitro kidney preservation techniques.


The United States social security system and Medicare and Medicaid.


Indiana State Medical Association.

Indiana State Medical Association.


The assessment of drug effects in children as compared to adults.


Healing of skin incisions closed by non-suture technique during the cicatrisation phase. A tensiometric and histologic study in the rat.


Selection of an antimotion sickness drug.


Pulmonary embolism and the urokinase pulmonary embolism trial.


Mobilization and examination of respiratory secretions for the diagnosis and treatment of chest diseases.


Obstetric practice and epidural analgesia.


Paracervical block anaesthesia for the evacuation of incomplete abortion--a controlled trial.


Secondary palatal impressions for major connector adaptation.


Making duplicate dentures for use as final impression trays.


Systems for classifying partially dentulous arches.


Single-tray dual-impression technique for distal extension partial dentures.


The cantilever type of posterior fixed partial dentures: a laboratory study.


The role of the maxillofacial prosthetist in support of the burn patient.


Behavior therapy in prosthodontics.

Behavior therapy in prosthodontics.


Duplicating tooth contours with castings.


Fabrication of an alloplastic cranioimplant.


Fabrication of an ocular prosthesis.

Fabrication of an ocular prosthesis.


A half century of Nashville medicine.

A half century of Nashville medicine.


Medicare and nursing homes: the changing scene.


The lonely Nietzsche and his migraine.

The lonely Nietzsche and his migraine.


Mycoplasma pneumoniae.

Mycoplasma pneumoniae.


Historic hospitals. The Order of the Holy Spirit.


Public health legislation and appropriations in North Carolina. A historical review.


Kidney transplantation using living donors.


Secondary syphilis misdiagnosed as lymphoma.


George Van S. and Olive Watkins Smith and the Free Hospital for Women, Brookline, Massachusetts.


Standards of early American medical education. II.


A simple hand dermatome.

A simple hand dermatome.


Effects of temperature reduction on multiple sclerosis.


Walter Walthall, M.D.: The man in control.


Immunology of transplantation.

Immunology of transplantation.


The aldehyde reaction with bilirubin of bile and of bile pigment in the icteric liver.


Vascular lesions in human allotransplantated kidneys.


Results of a biological experiment conducted aboard the automatic station "Zond-5".


Family therapy; an adjunct to hemodialysis and transplantation.


Resistance to infection of mice and hamsters following short term acceleration stress.


Pathogenesis of Junin virus infection in the infant hamster.


Immunofluorescence in pathogenesis of group A, B, and C arbovirus in the hamster.


The effect of vagotomy on human gastric secretion.


The problems of limb replacement.

The problems of limb replacement.


Extracorporeal perfusion on an arteriovenous shunt as a method of functional assessment of preserved kidneys.


The effect of Escherichia coli endotoxin on extracellular fluid volume.


The perfused rat heart as a model for tissue storage and viability.


An evaluation of rabbit anti-rat lymphocyte sera made from cells of differing viability.


Further studies of localized immunosuppression for canine pulmonary transplants.


Logarithmic time--a new dimension in organ preservation.


Immunosuppressive powers of the antibacterial agent trimethoprim.


The preservation of cadaveric kidneys for transplantation.


The effects of vagotomy upon the secretion of acid and pepsin in response to pentagastrin and insulin.


A method for improving the function of cadaveric porcine renal allografts.


Failure of capsulotomy to reduce deaths from renal ischaemia.


Organ preservation.

Organ preservation.


Liver transplantation.

Liver transplantation.


Osteonecrosis--a new dimension in degenerative arthropathy.


Calcification in vitro of collagenous model systems: chemical and electronmicroscopic aspects.


A clinical evaluation of mesoridazine in mentally retarded patients.


In vitro activity of gentamicin against common pathogenic bacteria.


The effects on human gastric secretion of prolonged continuous intravenous infusions of maximal and supramaximal doses of histamine acid phosphate and pentagastrin. The effects of continuous intravenous infusions of ;maximal' and ;supramaximal' doses of histamine acid phosphate and pentagastrin were assessed in a normal human volunteer. The secretory patterns in respect of the two drugs were indistinguishable over two and a quarter hours. During the steady states of acid secretion, outputs of pepsin and of electrolytes were also constant. The output of acid during the first hour of the steady state is a valid measurement of the maximal secretory capacity. The constant output of pepsin during the steady state of acid secretion suggests that both drugs are true stimulants of pepsin secretion.


Histopathology of skin allograft rejection in larval Rana catesbeiana.


Effects of histamine acid phosphate and pentagastrin on gastric secretion in normal human subjects. Six normal subjects were tested with various doses of histamine acid phosphate (1, 10, 40, and 80 mug/kg/hr) and pentagastrin (0.15, 1.5, 6.0, and 12.0 mug/kg/hr), the different doses of the stimulants being administered by separate continuous intravenous infusions. In each sample of gastric juice, which was collected at 15-minute intervals, we estimated the concentrations of the H(+), Na(+), K(+), and Cl(-) and the concentration of pepsin. The drugs elicited equal maximal outputs of acid and pepsin. Pentagastrin was more potent than histamine in stimulating acid and pepsin secretion, and the rate of the responses was faster with pentagastrin than with histamine. Apart from this, however, the patterns of secretion of the various constituents of the gastric juice and the interrelationships between the concentrations of the electrolytes were identical with the two drugs. We therefore concluded that the actions of histamine acid phosphate and pentagastrin on human gastric secretion were identical.


The forgotten surgeons.

The forgotten surgeons.


Cerebrovascular disease. A review of present understanding.


The crushed chest. Cardiovascular trauma.


Obituary. Ivan Bede Jose.

Obituary. Ivan Bede Jose.


Tolbutamide: hazards of leaks.

Tolbutamide: hazards of leaks.


Moliere and medicine.

Moliere and medicine.


2 anti-inflammatory components in antilymphocytic serum.


Scattered arrangement of the bacterial ribosomal cistrons.


Detection of actomyosin-type protein at the surface of dissociated embryonic chick cells.


The nasal respiratory handicap of expiratory airflow disease: the response to bronchodilator aerosols.


The fine structure of the mesonephros of the lamprey, Entosphenus japonicus Martens.


On the fine structure of the aglomerular renal tubule in Lophius piscatorius.


Change in stroke volume as related to change in total blood volume early after pulmonary surgery.


Kiel bone in the treatment of pseudarthrosis. An experimental study.


Operative elevation of the medial tibial joint surface in Blount's disease. One case observed for 18 years after operation.


A one-step method for orientation during embedding in plastic.


Primary cerebral reticulosarcoma.

Primary cerebral reticulosarcoma.


Short-term memory in the retarded: ability level and stimulus meaningfulness.


Harold Manville Skeels.

Harold Manville Skeels.


Maxillary arch correction in cleft lip and palate cases.


Suicide potential and time perspective.

Suicide potential and time perspective.


Differential response to chlorpromazine, imipramine, and placebo. A study of subgroups of hospitalized depressed patients.


Combined suture technique for corrective otoplasty.


Lung transplantation: problems of lung function.


The effect of age on the bone marrow cellularity of the mouse.


Joshua Clayton, Delawar physician during the Revolutionary period, 1776-1787.


Basic research in gerontology.

Basic research in gerontology.


The profession's plan.

The profession's plan.


Critical appraisal of the literature on deep carious lesions.


Appearance of virulent bacteriophages in lysogenic E. coli cultures after prolonged growth in the presence of triethylenemelamine. Continuous culture of coli 12lambda, P22, 600-434, 600-21, and 600-299 in the presence of triethylenemelamine (TEM) results in the appearance of a new virulent virus which attacks the parent culture. N-Methyl-N'-nitro-N-nitrosoguanidine (MNNG) is effective with 600-21 and ultraviolet light with 12lambda and 600-21. The cultures which produce the virulent virus continue to do so indefinitely in the absence of the mutagen, but are not lysogenic for the virus. Most of the cells in such cultures are resistant to the virus and do not produce any, but there are a few mutant cells sensitive to the virus and the virus multiplies by infection of these sensitive mutants.


Lytic agents, cell permeability, and monolayer penetrability.


Biochemical studies on active transport.


Serratia marcescens infections.

Serratia marcescens infections.


Electrical characteristics of tunicate heart cell membranes and nexuses. The tubular ascidian heart is composed of a single layer of cells joined together by apical (zonulae occludentes) and spot (maculae occludentes) nexuses. Intercalated discs or desmosomes were not observed in this tissue. Rectangular pulses of current were applied across the opened and flattened myocardium. Assuming that all the transepithelial current flowed through a uniform gap between cells, the resistivity in the gap must be very high compared to that in bulk solution. It is likely, therefore, that the gap width is of the order of an ionic radius or smaller. Assuming that all the transepithelial current flowed through the cells and that the inner and outer membranes had the same resistivity, the membrane resistivity was about 210 ohms cm(2) and the membrane capacitance was about 1.6 microF per cm(2). The myocardial cells were found to be in electrical continuity with each other through the nexuses since current could be passed through a strip of myocardium in a sucrose gap. Assuming that the longitudinal resistance of the cytoplasm was negligible, the cell-to-cell resistivity of the nexuses was 0.2 ohm cm(2). It is concluded that the nexuses provide a low resistance pathway between cells and a transepithelial barrier.


Chain initiation in primitive protein synthesis: a 60S intermediate in the formation of active 70S ribosomes.


Target lymphoid cell population of carcinogen induced immunodepression in mice.


Reactivity of anti-D immunoglobulin G subunits.


Loss of short term memory as a predictor of the alcoholic "blackout".


Anencephalus and the local water supply.


Kidneys against the law.

Kidneys against the law.


Immunosuppressive powers of the antibacterial agent trimethoprim.


Ketone bodies and evidence for increased insulin secretion.


Release of dopamine-beta-hydroxylase with norepinephrine during cat splenic nerve stimulation.


True antibodies to DNA in systemic lupus erythematosus: activity of Fab and F(ab')2 fragments.


DNA--RNA.

DNA--RNA.


Misuses for fructose.

Misuses for fructose.


Isolation and partial characterization of the transfer ribonucleic acid cistrons from Escherichia coli.


Division of chloroplasts in an artificial environment.


Effect of contraceptive pills on the distribution of sexual activity in the menstrual cycle.


Chloramphenicol misuse.

Chloramphenicol misuse.


Professor Thomas F. Goreau.

Professor Thomas F. Goreau.


Origins and growth of group practice.

Origins and growth of group practice.


Dr. Horace Wells, dentist, who first discovered, demonstrated and proclaimed the blessings of surgical anesthesia.


Human placental lactogen. An index of placental function.


Estimation of birth weight by quantified external uterine measurements.


Improved healing of experimental defects in the canine mandible by grafts of decalcified allogenic bone.


Surgical correction of bimaxillary protrusion and mandibular retrusion.


Immediate bone graft following resection of mandibular osteomyelitic lesion.


The chemistry of the porphyrins.

The chemistry of the porphyrins.


James Berney.

James Berney.


Hypertonic urea in craniotomy. A further report with special reference to acid-base status.


Lloyd Noland: sanitarian, surgeon and hospital administrator.


The emergence of modern periodontia.

The emergence of modern periodontia.


Remineralizing mouthwash rationale and a pilot clinical study.


Clinical management of osseous lesions.

Clinical management of osseous lesions.


Ex vivo evaluation of stored lungs.

Ex vivo evaluation of stored lungs.


Direct myocardial revascularization: saphenous vein bypass grafts to the distal coronary artery. Technical details and postoperative hemodynamics.


Congenital eventration of the diaphragm.


Clinico-pathological study of malignant lymphoma in Jamaica. A clinico-pathological study of malignant lymphoma in Jamaica was undertaken to examine the disease pattern in a predominantly negro population of West African origin. During a 9-year period (1958-66) 260 histologically verified cases of malignant lymphoma were encountered. The distribution of the different histological types was as follows: Hodgkin's disease 50.9%, lymphosarcoma 33%, reticulum cell sarcoma 14.2%, giant follicular lymphoma 1.9%. No cases of Burkitt's tumour were encountered.This study indicates that malignant lymphoma is not uncommon in Jamaica, and that its distribution pattern is similar to that observed in Europe and North America, except for the paucity of giant follicular lymphoma, and is different from the pattern observed in parts of Africa populated by Negroes, where Burkitt's tumour is the most common type, and where Hodgkin's disease is relatively uncommon. The age and sex incidence was in general similar to other reported series, but the duration of symptoms was short. The majority of patients presented with generalised peripheral lymphadenopathy. Hepatosplenomegaly and anaemia were common on admission. The prognosis was generally poor in comparison with European and North American series due to advanced stage of disease on presentation.


Primary reticulum-cell sarcoma of bone in Western India. Thirty-five cases of primary reticulum-cell sarcoma seen during 28 years are presented.These tumours occurred five times more frequently in males than in females, and were seen most commonly in adults. The femur was the commonest location. None of the tumours was located in the small bones of the hand, foot or skull bones (excluding maxilla).Radiation therapy has been the treatment of choice. Though the initial response was good, local recurrence and metastasis to regional nodes and generalised dissemination was not uncommon. Radiation therapy to regional nodes and concomitant chemotherapy are suggested to prevent metastasis in regional nodes and generalised dissemination.These tumours were found to have a spectrum of activity varying from a rapid fatal outcome within a few months to survival for many years.


The weight of the pineal gland in malignancy. A series of 150 pineal glands removed at routine postmortems in a general hospital have been examined. Statistical analysis of the weights of 147 of these glands from patients aged between 45 and 90 years, shows that the glands from patients dying of malignant disease are significantly lighter than those where the cause of death was non-malignant. These results are almost the exact reverse of those described recently in a similar series in America.After decalcification very little difference in the weight of the gland can be detected between the two groups and it would appear that the higher weight of the glands from non-malignant patients is due, at least in part, to the presence of a greater amount of mineral in these glands.


A quarter century of industrial hygiene surveys in the fibrous glass industry.


Surface counting in the assessment of sites of red cell destruction.


A special role of the group 17,18 chromosomes in reticuloendothelial neoplasia. The hypothesis is advanced that abnormalities of the chromosome group 17,18 play a special role in the genesis and/or evolution of some reticuloendothelial neoplasms. Aberrations of the group 17,18 chromosomes in tumour cells exceed in variety the reported anomaliesof any other chromosome. Both the frequency of these aberrations and their nature make them most unlikely to be due to chance. They appear to be non-random, often occurring in every cell of a tumour, and like the Ph(1) anomaly in chronic granulocytic leukaemia, mightpossess aetiological significance. The Ep- and Eqchromosomal anomalies resemble the Ph(1) in being fine structural modifications, which occur as acquired lesions only in neoplasms, often in tumour cells with otherwise normal karyotypes.Aberration of the group 17,18 chromosomes may sometimes be secondary to neoplasia but nevertheless of evolutionary significance for the tumour cells. Changes leading to relative or absolute excess of long-arm material of chromosome 18 may confer survival advantage upon cells, particularly if a normal complement of short-arm material is simultaneously retained. However, specific deletion of the distal part of the long arms of No. 18 may also favour cell survival. The short arms of chromosome 18 may carry genes limiting cell reproduction, while the long arms carry material promoting proliferation. More distally on the long arms, there may be genes which also limit reproduction. Disturbances affecting the balance between these components of the genome may be important in inception of neoplasia or subsequent evolution of tumour cell lines.


Early and late bone grafting in cases of cleft lip and palate.


The early management of bilateral cleft of lip and palate.


A critical appraisal of the Abbe flap in secondary cleft lip deformity.


The extended role of the delto-pectoral flap.


Total reconstruction of an avulsed upper lid.


Index to current urological literature.

Index to current urological literature.


Primary reticulum cell sarcoma of the penis.


Sexually transmitted disease in gynaecological out-patients with vaginal discharge.


Sexually transmitted disease in antenatal patients.


Use of vaccinia virus in the treatment of metastatic malignant melanoma. Of 19 patients with proved metastases from malignant melanoma treated by inoculations of smallpox vaccine, intradermal deposits disappeared completely in six out of ten cases. Five of these remained well 2 to 22 months after initial treatment. The response was limited strictly to the site of inoculation. The mechanism of action of vaccinia virus in malignant melanoma is not clear.


Use of radioactive fibrinogen for detecting rejection of human renal transplants. Radioactive (125)I-labelled human fibrinogen was given to 11 recipients of cadaveric renal transplants and the radioactivity of the transplant was measured with a scintillation counter. In the six patients with no increase in radioactivity over the transplants there was no evidence of rejection. The five patients with an increased transplant radioactivity all had other evidence of rejection at that time. In one patient the test detected rejection in the absence of any other signs, and this result was afterwards confirmed by renal biopsy. The test in one patient was invalidated by haemorrhage occurring from the site of a renal biopsy, and other possible errors in the test may arise.It is suggested that the technique may have particular value in recipients with delayed function of their transplants, but may also be useful in patients with functioning transplants in helping to confirm the diagnosis of rejection suspected on other grounds.


Autoimmunization with irradiated tumour cells in human malignant melanoma. Thirteen patients have been immunized with their own irradiated melanoma cells on one or more occasions. Following these autografts tumour-specific cytotoxic antibodies were produced, the longest response lasting 14 days. This procedure had no apparent effect on the course of the disease in these patients.


Comparison of pentazocine and pethidine in labour. A double-blind between-patient study was carried out comparing pentazocine with pethidine in 94 women in labour. Similar analgesic and sedative effects were obtained with the two drugs, and Apgar scores were similar for babies of both drug groups. Fewer emetic sequelae were reported after pentazocine injections, though there was no statistically significant difference between the two drug groups in incidence of other side-effects.


Long-term and short-term effects of oral prethcamide in chronic ventilatory failure. The effect of oral prethcamide (Micoren) (a mixture of two related amides of crotonyl N' butyric acid) was compared with a placebo preparation in 13 patients with established chronic ventilatory failure. Part I of the study comprised a double-blind single cross-over trial with an initial assessment and two further assessments at the end of each period of one month. Prethcamide was taken in 200-mg. doses four times daily. No subjective or objective changes were noted, and in particular the resting Pco(2) showed no change.Part II of the study comprised a double-blind single cross-over trial of the short-term effect of prethcamide compared with placebo in 12 patients in chronic ventilatory failure. Frequent estimations of mixed venous Pco(2) were made with a rebreathing technique for four and a half hours after ingestion of prethcamide or placebo preparation.Following prethcamide a fall in Pco(2) level to a minimum value at 30 minutes of 93% of control values and persisting for about three hours was noted for the group as a whole. The fall represents a lowering by about 4 mm. Hg of the mixed venous Pco(2).It is concluded that, though in patients with chronic ventilatory failure prethcamide may reduce the Pco(2) in the short term, there is no subjective benefit or observable objective change following repeated administrations over a period of one month.


Persistent behavioural and electroencephalographic changes after single doses of nitrazepam and amylobarbitone sodium. In a double-blind cross-over trial the effects of nitrazepam (5 and 10 mg.), amylobarbitone sodium (100 and 200 mg.), and placebo were compared in normal healthy young people. Though they reported a good night's sleep and adjudged themselves to be alert after all four drug treatments, behavioural tests showed their performance to be significantly impaired 13 hours after treatment with nitrazepam or amylobarbitone, and E.E.G. records showed more drowsiness and light sleep 18 hours after treatment with nitrazepam than with amylobarbitone or placebo. E.E.G. fast activity was more plentiful after drugs in either dosage than with placebo.


Evaluation of Simpson.

Evaluation of Simpson.


Phage typing of S.typhi-murium based on own observations. II. Assay of the Lilleengen system.


Renal homotransplant rejection associated with microangiopathic haemolytic anaemia.


Frederic D. Zeman, MD, 1894-1970.

Frederic D. Zeman, MD, 1894-1970.


A ninety-minute inquiry: the expressed needs of the elderly.


Inhibition of peptic activity by carbenoxolone and glycyrrhetinic acid. Carbenoxolone (Biogastrone, Berk) has been shown to reduce the peptic activity and total acidity of gastric juice obtained from anaesthetized pylorus-ligated rats without affecting significantly the volume of gastric juice secreted or the K(+) concentration. Glycyrrhetinic acid was less potent in reducing peptic activity and caused no reduction in total acidity. Antipeptic activity of carbenoxolone has also been demonstrated in vitro using the pepsin plate technique and the haemoglobin pepsin assay.It is suggested that these actions of carbenoxolone may contribute to the increased rate of healing of peptic ulcer in patients treated with the drug.


Peritoneal macrophages in the immune response to SRBC in vitro.


On antibodies to gastrin: concerning their production, behavioural characteristics, and uses.


Quantitative studies of immunofluorescent staining. IV. Indirect immunofluorescence blocking tests with anti-perinuclear factors of human and goat origin.


Conjugation of fluorescein isothiocyanate to antibodies. II. A reproducible method.


Stability studies on indocyanine green dye.


Effects of RES "blockade" on antibody formation. 3. Proliferative capacity of antigen-reactive stem cells in carbon-treated mice after transfer to irradiated recipients.


Reticulum cell sarcoma of the nose and paranasal sinuses.


Mandibular replacements.

Mandibular replacements.


Recent concepts in the management of temporomandibular joint dysfunctions.


Johan Frederick, Count Struensee, M.D. (1737-1772). King, Queen, Jack.


Dr. Moore begins retirement after 23 years in Sitka.


Role of microenvironment in trophoblastic tissue growth and invasion.


Effects of some vasopressor drugs upon uterine contractility in pregnant women.


A simple midcervical cerclage operation for cervical incompetence during pregnancy.


Intrauterine diagnosis of fetal diaphragmatic hernia by amniography.


Keratoconjunctivitis test in Shigella flexneri 2A hybrids obtained in a cross with different strains of E. coli K12.


Effects of infection on selected clinical and biochemical parameters in dogs.


Evaluation of Simpson.

Evaluation of Simpson.


The surivival of Staphylococcus aureus on human skin. An investigation using mixed cultures.


The effect of parenteral iron preparations on experimental pyelonephritis.


Biological activity of drugs. X. Relation of structure to the bacteriostatic activity of sulfonamides. (1).


The nature and treatment of stress ulcers. A review.


Biological activity of drugs. XI. Relation of structure to the bacteriostatic activity of sulfonamides. (2).


Problems in cadavaric donations for renal transplantation.


Medical Grand Rounds Yale-New Haven Hospital. Chronic renal failure treated by transplantation.


Diverticular disease of the colon--a disease of western civilisation.


Milk-coagulation and proteolysis. I.

Milk-coagulation and proteolysis. I.


Milk-coagulation and proteolysis. II.

Milk-coagulation and proteolysis. II.


On proteolytic enzymes from the fig.

On proteolytic enzymes from the fig.


Inhibition of apomorphine-induced vomiting by benzquinamide.


The clinical pharmacology and therapeutic applications of the antiarrhythmic drugs.


Butabarbital sodium and chlordiazepoxide in anxious neurotic outpatients: a collaborative controlled study.


Seminole Indian Medicine.

Seminole Indian Medicine.


Thalidomide in the treatment of erythema nodosum leprosum. With a note on selected laboratory abnormalities in erythema nodosum leprosum.


Government medical service to the Seminole Indians.


Josie Billie, Seminole Doctor, Medicine Man and Baptist Preacher.


The Chinese diagnostic doll.

The Chinese diagnostic doll.


Homatropine bradycardia in man.

Homatropine bradycardia in man.


In memory of Dr. Francis P. Wellford.

In memory of Dr. Francis P. Wellford.


Comparisons of effects of morphine, meperidine, fentanyl, and fentanyl-droperidol.


Early Florida and organized medicine.

Early Florida and organized medicine.


Tympanoplasty--the posterior wall. 1. Reconstruction with experimental materials.


Tympanoplasty--the posterior wall. 3. Tympanoplasty--a review of present thinking.


The okuu--Hawaii's greatest epidemic.

The okuu--Hawaii's greatest epidemic.


Editorial. "The old order changeth, yielding to the new...".


Epithelial-mesenchymal interactions related to periodontal disease.


Clinical evaluation of Pulsar, a new pulsating water pressure cleansing device.


Effect of epinephrine and mannoheptulose on early and late phases of glucose-stimulated insulin release.


Effect of L-asparaginase on carbohydrate metabolism.


Roza Kuleshova found peeping.

Roza Kuleshova found peeping.


Breeding cattle for the tropics.

Breeding cattle for the tropics.


Central dogma of molecular biology.

Central dogma of molecular biology.


Protein-deficient ribosomes in chloramphenicol-treated Escherichia coli.


Segregation of transferable R factors into Escherichia coli minicells.


Natural release of nucleic acids from bacteria into plant cells.


Dissociation of beta-galactosidase by thiols.


Monosodium glutamate and the Chinese restaurant syndrome.


Scientific heresy.

Scientific heresy.


Modes of transmission of whirling disease of trout.


Effect of role-empathy on height of human figures drawn by male alcoholics.


Comparison of competitive protein binding radioassay of cortisol to double isotope dilution and Porter Silber methods.


The larynx, organ of voice by Julius Casserius. Translated from the Latin with preface and anatomical notes.


Structure, conformation, and mechanism in the formation of polysaccharide gels and networks.


Frederic Parker, Jr. MD, 1890-1969.

Frederic Parker, Jr. MD, 1890-1969.


Clinical significance and roentgen appearance of the gastric pouch and gastroduodenal anastomosis following truncal vagotomy with antrectomy.


Superior vena caval syndrome caused by intrathoracic goiter.


Microangiopathic hemolytic anemia.

Microangiopathic hemolytic anemia.


Leukocytic mechanisms in inflammation.

Leukocytic mechanisms in inflammation.


Human insulin secretion: as integrated approach.


Hypo-osmolar syndromes secondary to impaired water excretion.


Edema.

Edema.


Crystal-induced inflammation of the joints.


Chemotherapy of mental depression.

Chemotherapy of mental depression.


Regulation of the secretions of the pancreas.


Human chromosome damage by chemical agents.


L-asparaginase.

L-asparaginase.


Immunological methods in brucellosis research. II. In vivo procedures.


Fluorescein fundus photography (F.F.Ph.). A five year review (1960-1965).


Controlled clinical trial of ergotamine tartrate. A double-blind controlled clinical trial of cross-over design for the treatment of headache was conducted in 88 women identified during a community survey as having headaches with the features of migraine. Of 79 subjects who completed the trial, 40 benefited from oral ergotamine tartrate and 46 benefited from the placebo. There was no evidence that ergotamine in doses of 2 or 3 mg. was more effective than the placebo. Ergotamine aggravated the attack significantly more often than the placebo. Neither the colour of the tablets nor the order of therapy significantly affected the results of the treatment.


Nifuratel compared with metronidazole in the treatment of trichomonal vaginitis. Nifuratel (Magmilor) was compared with metronidazole (Flagyl) in the treatment of trichomonal vaginitis by a randomized double-blind trial. Only 18 out of 47 patients (38%) treated with nifuratel were found to be cured, whereas 42 out of 49 patients (85%) treated with metronidazole were cured. Severe reactions, necessitating withdrawal of treatment, occurred in three patients treated with nifuratel. There were no serious side-effects with metronidazole. The results of this trial indicate that nifuratel is not a satisfactory substitute for metronidazole in the treatment of trichomoniasis.


The design of drug trials with neurotic patients.


The severely ischemic leg.

The severely ischemic leg.


Motility of the digestive tract after administration of medium chain triglycerides (MCT) as compared with long chain triglycerides (LCT).


1970 Roster of the Greater St. Louis Dental Society.


Effects of alloxan upon goldfish (Carassius auratus L.).


The cardiovascular effects of anxiety and R-epinephrine retraction cord in routine fixed prosthodontic procedures.


Macromolecular subunits in the walls of marine photosynthetic bacteria. Electron micrographs of sectioned and frozen-etched marine species of Chromatium revealed that at least two different patterns of macromolecular subunits are present on the cell wall.


Dr. Matthew Walker honored.

Dr. Matthew Walker honored.


Membership roster.

Membership roster.


Appetite stimulation and weight gain with cyproheptadine. A double-blind study in underweight children.


Intermittent positive-pressure breathing.


Fabrication of a crown or inlay under an existing clasp.


Multiplication of bacteriophage P1 mutants in Shigella dysenteriae strain Sh(P1). From bacteriophage P1, 10 mutants (P1cl) were isolated which are impaired in their ability to lysogenize Shigella dysenteriae Sh and which fail to make plaques when plated on Sh(P1). When Sh(P1) is infected with P1cl, a considerable proportion of the infected cells is converted into infectious centers, which eventually release P1cl but not P1. This phage release occurs over a period of several hours, during which a manyfold multiplication of infectious centers takes place. In the course of this multiplication, surviving bacteria, lysogenic for P1 only, are produced by segregation. At high multiplicity of infection, Sh(P1) are killed without producing any phage.


Renal disease with Staphylococcus albus bacteremia. A complication in ventriculoatrial shunts.


Aspergillosis. The spectrum of the disease in 98 patients.


Has social change influenced projective drawings and their interpretation?


Graeme Campbell Taylor Burns.

Graeme Campbell Taylor Burns.


L.P.N. to R.N. the associate degree way.


Drug photosensitivity. I. Light- and photo-sensitivities observed during oral contraceptive therapy. A review.


Immunosuppressive drugs.

Immunosuppressive drugs.


Chronic beta adrenergic receptor blockade in the treatment of idiopathic hypertrophic subaortic stenosis.


Postoperative intensive care in the cardiac surgical patient.


Ulcerative colitis.

Ulcerative colitis.


Anterior mediastinitis as a complication of median sternotomy incisions: diagnostic and surgical considerations.


Successful 24 hour renal perfusion-preservation with monitoring by surface electrometry during the storage interval.


Theory of blood coagulation: applications in disseminated intravascular coagulation.


Glucocorticoids in haematology.

Glucocorticoids in haematology.


Reticulum cell sarcoma of the thyroid--a case report.


Factors controlling apatite crystallization, with particular reference to the effect of fluoride and accompanying ions.


A biophysical approach to epithelial cell interactions with teeth.


Microradiography of oral tissues.

Microradiography of oral tissues.


Biochemistry of the dental plaque.

Biochemistry of the dental plaque.


Antigens of oral bacteria.

Antigens of oral bacteria.


Extracellular beta-lactamase from Escherichia coli.


Repair in the human dental pulp following cavity preparation.


Vacuolar dynamics in synchronously budding yeast.


Quantitative determination of antibacterial activity in the rabbit gingival sulcus.


Mark Akenside, 1721-70.

Mark Akenside, 1721-70.


Early breast cancer.

Early breast cancer.


Premedication with droperidol: a double-blind study.


Poisoning of mink by spoiled feed contaminated with Escherichia coli and Paracolobactrum spp.


A historical note: Leeuwenhoek's description of crystals from a gouty tophus.


Microchromatography and microelectrophoresis on nitrocellulose membranes.


The determination of amino acids in plasma and urine by ion-exchange chromatography.


Present chromatographic methods of fractionating DNA.


Chromatography of triazines.

Chromatography of triazines.


Psychophysical studies of temperature sensitivity.


Cat superior olive S-segment cell discharge to tonal stimulation.


Vision, audition, and beyond.

Vision, audition, and beyond.


Anatomical aspects of the cochlear nucleus and superior olivary complex.


Task force urges major revamping of medicare.


Psychological methods applied to evaluate symptomatic geriatric treatment.


Amino acid control over deoxyribonucleic acid synthesis in Escherichia coli infected with T-even bacteriophage. Starvation for a required amino acid of normal or RC(str)Escherichia coli infected with T-even phages arrests further synthesis of phage deoxyribonucleic acid (DNA). This amino acid control over phage DNA synthesis does not occur in RC(rel)E. coli mutants. Heat inactivation of a temperature-sensitive aminoacyl-transfer ribonucleic acid (RNA) synthetase similarly causes an arrest of phage DNA synthesis in infected cells of RC(str) phenotype but not in cells of RC(rel) phenotype. Inhibition of phage DNA synthesis in amino acid-starved RC(str) host cells can be reversed by addition of chloramphenicol to the culture. Thus, the general features of amino acid control over T-even phage DNA synthesis are entirely analogous to those known for amino acid control over net RNA synthesis of uninfected bacteria. This analogy shows that the bacterial rel locus controls a wider range of macromolecular syntheses than had been previously thought.


Segregation into and replication of plasmid deoxyribonucleic acid in chromosomeless segregants of Escherichia coli. The progeny cells of Escherichia coli strain P678-54, which normally do not contain deoxyribonucleic acid (DNA), were found to carry DNA when the parental cell carried colicin factor E1 (Col E1). The DNA found in the normally DNA-less segregants was shown to be Col E1 DNA, which is present primarily as a covalently closed circular molecule that can undergo more than one complete cycle of replication.


Role of pili in bacterial conjugation.

Role of pili in bacterial conjugation. We describe techniques for isolating individual pairs of mating Escherichia coli and observing them under the light microscope. Some pairs achieved close cell-to-cell contact, whereas others remained loosely connected by invisible connections which may be F pili. After 30 min of mating, the pairs were separated and allowed to grow into clones. That many exconjugants derived from "loose"-mating pairs produced recombinants suggests that F pili are involved in the transfer of genetic material. The frequency of formation of recombinants from "close"-mating pairs, however, was significantly higher than that from loose-mating pairs, indicating that a close cell-to-cell contact facilitates chromosome transfer. Death rates of exconjugants from close pairs were also higher than those from loose pairs. Hfr x F(-) matings produced higher death rates than F(+) x F(-) matings. Male cells were found capable of transferring genetic markers to two F(-) cells simultaneously. We conclude that F pili play at least three roles in mating: (i) they initiate contacts between mating pairs; (ii) they facilitate the transfer of genetic material; and (iii) they draw mating cells into a close contact which increases the fertility of the union.


Reversion of the frameshift hisD3018 of Salmonella typhimurium. Previous studies on histidinol dehydrogenase from His(+) revertants have shown that the frameshift mutation hisD3018 is a +1 type, resulting from inclusion of an extra cytidylate residue in messenger ribonucleic acid. Histidinol dehydrogenase from newly isolated spontaneous and N-methyl-N'-nitro-N-nitrosoguanidine (NG)-induced intragenic revertants has been examined for amino acid replacements. The results provide additional evidence that NG can delete guanine plus cytosine base pairs from deoxyribonucleic acid. One spontaneous revertant was found to result from a +2 addition of approximately 16 nucleotide residues before the +1 parent frameshift, and another by a -4 deletion about six residues before the same. Circumstantial evidence suggests the in vivo codon assignment GAG for glutamic acid. A region of histidinol dehydrogenase highly permissive of amino acid changes encoded in the minus (-) phase is now apparent.


Genetic regulation of ribonucleoside and deoxyribonucleoside catabolism in Salmonella typhimurium. Four enzymes involved in ribonucleoside and deoxyribonucleoside catabolism (deoxyribose-5-P aldolase, thymidine phosphorylase, phosphodeoxyribomutase, and purine nucleoside phosphorylase) are coded for by four closely linked structural genes on the Salmonella chromosome. The genetic order of these genes is (deoC-deoA-deoB-deoD)-serB-thr. Studies on polarity mutants and induction patterns indicate that the deoB and deoD genes may constitute a single operon and that the deoC and deoA genes may constitute a second closely linked operon.


Synergism between cysteine and alkylating agents. Contrary to expectation, l-cysteine did not protect Escherichia coli from the lethal action of two monofunctional alkylating agents (nitrosomethylurethane and methylmethane sulfonate). The antibacterial action of these compounds was actually greatly enhanced by l-cysteine. This synergistic effect was also exhibited, to some extent, by d-cysteine but not by homocysteine, S-methylcysteine, or serine. The synergistic action between methylating agents and l-cysteine was not due to the formation of S-methylcysteine. l-Cysteine had no effect on the bacteriostatic action of ethylmethane sulfonate.


Polysome stability in relaxed and stringent strain of Escherichia coli during amino acid starvation. The influence of amino acid starvation on polysome content was examined in relaxed and stringent strains of Escherichia coli which were isogenic for the RC locus. No difference was observed between the polysome profiles obtained from two different sets of stringent and relaxed strains starved for the same amino acid. In both relaxed and stringent strains, starvation for amino acids other than methionine resulted in only a slight breakdown of polysomes with a concomitant increase of 70S ribosomes. However, starvation for methionine in both RC stringent and relaxed strains of E. coli resulted in a more extensive degradation of polysomes and accumulation of 70S ribosomes. The 70S ribosomes obtained as a result of methionine starvation were more sensitive to degradation to 50 and 30S subunits in 10(-3)m Mg(2+) than 70S monomers obtained either by degradation of polysomes with ribonuclease or by starvation of cells for amino acids other than methionine. The 70S ribosomes from methionine starvation were similar (sensitivity to 10(-3)m Mg(2+)) to 70S ribosomes obtained from cells in which initiation of protein synthesis had been prevented by trimethoprim, an inhibitor of formylation. Since N-formyl-methionyl-transfer ribonucleic acid is required for initiation, the 70S ribosomes obtained in both methionine-starved and trimethoprim-treated cells must result from association of 50 and 30S subunits for reasons other than reinitiation. These results suggest that the level of ribonucleic acid synthesis does not influence the distribution of ribosomes in the polysome profile and vice versa.


Mechanism of action of nalidixic acid on conjugating bacteria. When nalidixic acid, a specific and effective inhibitor of cellular deoxyribonucleic acid synthesis, is added to conjugating bacteria at any time during mating, it stops genetic transfer provided the donor bacterium is sensitive to the drug. When this inhibition is released by the removal of the nalidixic acid, transfer does not resume at the point on the chromosome where it was stopped, but begins again at the transfer origin. Curves relating the effects of various low doses of nalidixic acid to the frequency of recombination reveal that several "hits" are necessary to inhibit recombination for early markers. The number of required "hits" decreases as the distance of the marker from the transfer origin increases. Transfer between drug-resistant cells may also be inhibited by nalidixic acid. The effect of high drug doses on matings between resistant cells is similar to that of low drug doses on matings with a sensitive male.


Effect of manganese and zinc ions on the growth of Rhizobium. Five strains, representing four species of Rhizobium, were grown in specially purified synthetic culture solutions with manganese or zinc ion concentrations ranging from 0 to 10(-5)m. Omission of Mn(2+) restricted growth to 0.4 to 10% of that of controls, depending on the species. Growth was generally maximum with 10(-6)m Mn(2+) and nearly so with 10(-5)m. Omission of Zn(2+) restricted growth to 1 to 20% of that of controls. Growth was generally maximum with 10(-7)m Zn(2+). The 10(-5)m level of Zn(2+) was toxic to a variable degree to all species, suppressing the growth of one strain of R. meliloti to 49% of that of controls, and of R. phaseoli to only 0.4% of that of controls. A Mn(2+) x Zn(2+) interaction was also obtained as evidenced by a higher apparent Zn(2+) requirement with 10(-7)m Mn(2+) than with 10(-6)m Mn(2+).


Continued expression of the ribonucleic acid control gene during inhibition of Escherichia coli ribonucleic acid and protein synthesis. The effect of the ribonucleic acid (RNA) control (RC) gene on the biosynthesis of viral RNA has been examined in an RC(str) and an RC(rel) host infected with R17 RNA bacteriophage under conditions in which host RNA and protein synthesis were inhibited by the addition of rifampicin. Methionine and isoleucine starvation depressed viral RNA biosynthesis in an RC(str) host but not in an RC(rel) host. However, histidine starvation had little effect on viral RNA and protein synthesis in both RC(str) and RC(rel) cells, although it had a marked effect on host protein and RNA synthesis in an RC(str) host. Chloramphenicol relieved the effect of amino acid starvation on viral RNA synthesis in an RC(str) host. It is concluded that stringent control of viral RNA biosynthesis does not require the continued biosynthesis of the RC gene product (RNA or protein) and that a preformed RC gene product can regulate the biosynthesis of the exogenous RNA. It is suggested that the amino acid dependence of viral RNA biosynthesis is due to its obligatory coupling with the translation of the viral coat protein which lacks histidine. It may be inferred that the amino acid requirement of bacterial RNA is due to its coupling with the translation of a host-specific protein (other than the RC gene product) which requires a full complement of amino acids. Since chloramphenicol is known to permit ribosome movement in the absence of protein synthesis, it is suggested that ribosome movement along the nascent RNA chain is a sufficient condition for the continuation of RNA synthesis.


Malate dehydrogenase mutants in Escherichia coli K-12. Mutants devoid of malate dehydrogenase activity have been isolated in Escherichia coli K-12. They do not possess detectable malate dehydrogenase when grown aerobically or anaerobically on glucose as sole carbon source. All mutants revert spontaneously; a few partial revertants have been found with a malate dehydrogenase exhibiting altered electrophoretic mobility. Therefore, only one such enzyme appears to exist in the strains examined. No evidence could be obtained for the presence of a malate dehydrogenase not linked to nicotinamide adenine dinucleotide. Mutants deficient in both malate dehydrogenase and phosphoenol pyruvate carboxylase activities will grow anaerobically on minimal glucose plus succinate medium; also, malate dehydrogenase mutants do not require succinate for anaerobic growth on glucose. The anaerobic pathway oxaloacetate to succinate or succinate to aspartate appears to be accomplished by aspartase. Malate dehydrogenase is coded for by a locus somewhere relatively near the histidine operon, i.e., a different chromosomal location than that known for other citric acid cycle enzymes.


Regulation of ribonucleic acid synthesis in Escherichia coli during diauxie lag: accumulation of heterogeneous ribonucleic acid. The synthesis of ribonucleic acid (RNA) and of protein in Escherichia coli during glucose-lactose diauxie lag have been examined. The rate of RNA synthesis is about 7%, of the corresponding rate during exponential growth and the rate of protein synthesis 10 to 15%. Inhibition of RNA synthesis occurs to the same extent in both rel and rel(+) strains. The RNA which accumulates during 20 min in diauxie lag is composed of about 50% ribosomal and transfer RNA species and about 50% of a fraction which resembles messenger RNA (mRNA) in its heterogeneous sedimentation properties. Decay of the heterogeneous fraction occurs in the presence of glucose and actinomycin D with a half-life of 3 min, the same as that of pulse-labeled mRNA; however, during the diauxie lag, the half-life of this RNA is about 25 min. Accumulation of the heterogeneous RNA is further increased when protein synthesis is blocked by chloramphenicol. The data suggest that the disproportionate accumulation of mRNA during diauxie lag and energy source shift-down may be attributed at least in part to increased stability of mRNA, but do not rule out a preferential synthesis of mRNA.


Inhibition of the induced formation of tryptophanase in Escherichia coli by near-ultraviolet radiation. Induced formation of tryptophanase in Escherichia coli B/r is temporarily inhibited by near-ultraviolet (UV) irradiation. The inhibition is greater when irradiation is at 5 C than when at room temperature. Hence, the inhibition is the result of a photochemical, rather than photoenzymatic, alteration of some cellular component. The action spectrum has a peak in the region of 334 nm and is similar to that for growth delay. However, inhibition of tryptophanase formation is more sensitive to near-UV irradiation than are growth, respiration, and the induced formation of beta-galactosidase. Thus, for tryptophanase the lack of formation cannot be due to general inhibition of metabolism. Pyridoxal phosphate absorbs in the near-UV region of the spectrum and is a cofactor for tryptophanase, but this enzyme in induced cells is not inactivated by near UV-radiations. An experiment in which toluene-treated suspensions from irradiated and unirradiated cells were mixed showed that irradiation does not cause the formation of an inhibitor of tryptophanase activity. The possibility remains that the absorption of radiant energy by pyridoxal phosphate interferes with the synthesis of tryptophanase.


Biosynthesis of 4-aminobenzoate in Escherichia coli. Two different mutations (pabA and pabB) affecting 4-aminobenzoate biosynthesis were obtained in strains of Escherichia coli lacking chorismate mutase and anthranilate synthetase activity, thus allowing study of the pathway of biosynthesis of 4-aminobenzoate by use of cell extracts of strains carrying the pab mutations. Two components with approximate molecular weights of 9,000 (component A) and 48,000 (component B) are concerned in the biosynthesis of 4-aminobenzoate from chorismate by E. coli. No diffusible intermediate compound could be detected.


Deoxyribonucleic acid synthesis and cell division in a lon- mutant of Escherichia coli. The lon(-) mutants of Escherichia coli form long filamentous cells after temporary inhibition of deoxyribonucleic acid (DNA) synthesis by ultraviolet irradiation, treatment with nalidixic acid, or thymine starvation. The kinetics of DNA synthesis and cell division after a period of thymine starvation have been compared in lon(+) and lon(-) cells. After this treatment, both kinds of cells recover their normal DNA to mass ratio with the same kinetics. In contrast to previous reports, cell division is found to recommence in both lon(+) and in lon(-) cells after such a temporary period of inhibition of DNA synthesis. However, the delay separating the recommencement of DNA synthesis and of cell division is approximately three times as long in lon(-) as in lon(+) cells. Low concentrations of penicillin inhibit cell division in both lon(+) and lon(-) cells. In this case, cell division recommences with the same kinetics in both strains after the removal of penicillin. This suggests that different steps in the cell division process are blocked by inhibition of DNA synthesis and by penicillin treatment. The lon(-) mutation appears to affect the former of these steps.


Glycerol kinase, the pacemaker for the dissimilation of glycerol in Escherichia coli. The activity of glycerol kinase is rate-limiting in the metabolism of glycerol by cells of Escherichia coli. A mutant strain producing a glycerol kinase resistant to inhibition by fructose-1,6-diphosphate grows faster than its wild-type parent on glycerol as the sole source of carbon and energy. The amount of intracellular fructose-1,6-diphosphate was determined for wild-type cells growing exponentially on glycerol. The water content of such cells was also determined, allowing calculation of the intracellular concentration of fructose-1,6-diphosphate. This value, 1.7 mm, is adequate to exert substantial inhibition on the wild-type glycerol kinase. The desensitization of glycerol kinase to feedback inhibition also enhances the power of glycerol to exert catabolite repression, both on the enzymes of the glycerol system itself and on those of the lactose system. However, desensitization of glycerol kinase alone does not eliminate the phenomenon of diauxic growth in a glucose-glycerol medium. Biphasic growth in such a medium is abolished if the altered enzyme is produced constitutively. The constitutive production of the mutant kinase at high levels, however, renders the cells vulnerable to glycerol. Thus, when the cells have been grown on a carbon source with a low power for catabolite repression, e.g., succinate, sudden exposure to glycerol leads to overconsumption of the nutrient and cell death.


Morphology and ultrastructure of normal rod-shaped and filamentous forms of Erwinia amylovora. Filamentous cells of Erwinia amylovora were usually 3 to 35 times longer than the normal rod-shaped ones, and only the former produced minicells.


K plus-dependent deplasmolysis of a marine pseudomonad plasmolyzed in a hypotonic solution. When cells of a marine pseudomonad were washed with a solution consisting of 0.3 m NaCl, 0.05 m MgSO(4), and 0.01 m KCl (complete salts), they maintained their normal morphology. When washed with a solution of 0.05 m MgSO(4), they became plasmolyzed as indicated by both phase and electron microscopy. Suspensions of cells washed with 0.05 m MgSO(4) showed an increase in optical density (OD) when 0.3 m NaCl was added, and this was followed by a decrease in OD upon the further addition of 0.01 m KCl. Salts of other monovalent cations were not effective in replacing K(+) in producing the OD decrease. Phase-contrast microscopy revealed that the increase in OD was accompanied by a decrease in cell size, and the decrease in OD, by an increase in the cell size. Both phase and electron microscopy showed that the K(+)-dependent decrease in OD was accompanied by deplasmolysis of the cells. Na(+) was required in the suspending medium in addition to K(+) to obtain deplasmolysis. The intracellular K(+) concentration in cells which had been washed with complete salts and which had retained their normal morphology was found to be 0.290 m. In cells plasmolyzed by washing with 0.05 m MgSO(4), the intracellular K(+) concentration was 0.004 m. Deplasmolyzed cells contained 0.330 m K(+). The membrane profile of plasmolyzed cells was retained when protoplasts were formed. The protoplasts became spherical if incubated in a solution permitting the deplasmolysis of the parent cells. The evidence obtained indicates that plasmolysis and deplasmolysis under the conditions described was due to the loss and gain, respectively, of K(+) by the cells. The effect of Na(+) could be ascribed to its capacity to control the porosity of the cytoplasmic membrane of this organism.


Ultrastructural features of Mycoplasma pneumoniae. The ultrastructure of Mycoplasma pneumoniae cultivated in broth on glass and plastic surfaces was studied by scanning and transmission electron microscopy. The organisms grew as filaments, which by over-crossing eventually formed a dense network on the surface and in colonies composed mainly of rounded and elongated forms. The filaments were usually thinner at the ends and terminated with a knob-like structure. Some filaments possessed short ramifications which also ended with a knob, and others showed constrictions. Sectioned organisms were seen to contain ribosome-like structures. Many organisms had a specialized structure at their thinner end, which consisted of a dense rod surrounded by electron-lucent cytoplasm and ending with a platelike thickening.


Identification of the purI locus in Escherichia coli K-12. A genetic locus has been identified in Escherichia coli that is analogous to the purI locus in Salmonella.