Enzyme pattern of macrophages in the course of inflammation.


Sodium chlorate bomb injuries of the hand.


A new technique for metacarpal hand treatment.


Retiring editor. Mr. A. B. Wallace, C.B.E.


Reconstruction of the vulva--a new approach.


The tubed pedicle flap.

The tubed pedicle flap.


Some aspects of plastic (cosmetic) surgery in orientals.


An improved appliance for the pre-surgical orthodontic preparation of the cleft case for secondary bone grafting.


Centenary commemoration of sarcoidosis and of Jonathan Hutchinson.


Anencephalus and spina bifida.

Anencephalus and spina bifida.


Erythraemia in renal transplantation.

Erythraemia in renal transplantation. Seven cases of erythraemia following successful renal transplantation are described. This complication was not necessarily associated with clinical evidence of rejection of the transplant, and the prognosis so far as rejection was concerned was excellent. Four patients, however, developed thromboembolic pulmonary complications, and two other patients had thrombotic episodes. Hence it is suggested that treatment either by anticoagulation or by venesection is indicated.


Recognition of unwanted drug effects.

Recognition of unwanted drug effects.


A new technique for the recovery of Fasciola gigantica eggs from cattle faeces.


Now and then.

Now and then.


The radiological investigation of a cerebral tumour suspect.


A survey of post mortems, Malta hospital 1829-1838. With special reference to the cholera epidemic of 1837.


A trial of oral dehydroemetine compounds in the treatment of amoebiasis.


Richard Powell (1767-1834).

Richard Powell (1767-1834).


Response in two groups of anergic patients to the transfer of leukocytes from sensitive donors.


Age at menarche. Genetic and environmentalinfluences.


Immune-complex disease.

Immune-complex disease.


New drugs in the treatment of angina.

New drugs in the treatment of angina.


Progress in leprosy.

Progress in leprosy.


Centennial of the founding of the children's hospital in Boston.


Dr. James W. Goddard.

Dr. James W. Goddard.


Infection and quantitative recovery of Salmonella typhimurium and Escherichia coli from within the lesser mealworm, Alphitobius diaperinus (panzer).


Correlation between the breakdown of deoxyribonucleic acid and radiosensitivity of Escherichia coli.


Organic peroxides and the antibacterial action of irradiated sucrose as affected by catalase.


Survey observations on ovine mastitis in New Zealand stud Romney flocks. 2. The bacteriology of ovine mastitis.


The effects of extracorporeal irradiation of circulating blood and thoracic duct lymph on tetanus antitoxin responses in calves.


Effects of bacterial endotoxin on operant behavior of the rat.


Preliminary animal toxicology and human tolerance of antiherpes agent U-3243.


Experimental studies on the interaction between anti-type B streptococcal antibody and animal heart tissue.


Fungal contamination of donor eyes.

Fungal contamination of donor eyes.


Fredrick Herman Verhoeff, 1874-1968.

Fredrick Herman Verhoeff, 1874-1968.


Serologically detected RtH-1 antigens of the AS2, VM and Y 59 rats.


Cultivation of lymphoid cells in a chamber employing continuous flow of blood plasma of extracorporeal circuit. IV. The primary antibody response.


Fluorescent antibody tests in pre-clinical experimental Mycobacterium johnei infections in cattle.


Diffusional resistance of the innermost layer of the placental barrier of the rabbit. 1. The permeability of the endothelial cell layer of the foetal capillaries of the rabbit placenta was measured by the tracer-dilution method. Isotopically labelled water, urea, chloride and sodium ions, and inulin were used. Radio-iodinated human serum albumin served as reference tracer. The measurements were made in in situ placentas of 27-29 days gestation of which the foetal circulation was perfused with homologous blood.2. The permeability of water was found to be 1.7 ml. min(-1).g(-1), the permeabilities of urea, chloride ions and sodium ions were in the range of 0.28-0.42 ml. min(-1).g(-1) and the permeability of inulin was 0.053 ml. min(-1).g(-1). These values may be underestimates, particularly the value of water.3. The approximate maternal blood space, foetal blood space, and extravascular sodium space were determined in rabbit placentas of 27-29 days gestation and found to be 0.16, 0.07, and 0.48 ml./g respectively.4. The permeabilities of the endothelial layer were compared to those published for the entire placenta and found to be far greater. The resistance to diffusion of the endothelial layer is only a small part of the total resistance to diffusion. Most of the resistance is localized in the syncytioand/or cellular trophoblasts.5. It was concluded that lipid insoluble molecules diffuse through the interstitial water spaces of the trophoblast in few, relatively wide channels but that diffusing water can make use of intracellular as well as extracellular paths.


John Wolcot, MD (1738-1819). Peter Pindar, the satirist.


Researching a historical book.

Researching a historical book.


Thomas Campion: lyrick-doctor in physicke.


Jan van Riemsdyk, medical illustrator extraordinary.


The introduction of printing into Italy.


Alexander Borodin, the scientist, the musician, the man.


Medical explorers in Africa.

Medical explorers in Africa.


"Urine is an harlot, or a lier".

"Urine is an harlot, or a lier".


Reading for a desert island.

Reading for a desert island.


Tobias Smollett (1721-1771) Scottish novelist.


The vascular stapler in transplantation surgery.


Antimitochondrial antibodies in jaundice following drug administration.


Chaucer and medicine.

Chaucer and medicine.


Hair transplantation.

Hair transplantation.


La traviata.

La traviata.


The legacy of Dr. Oliver Wendell Holmes.


Identification of pathogenic fungi in paraffin embedded tissue sections by means of the fluorescent antibody technic.


Food-borne epidemic of streptococcal pharyngitis at the United States Air Force Academy.


Bronchiolar emphysema and related diseases.


Coxsackie viruses and the heart.

Coxsackie viruses and the heart.


Validation of dextran blue as a dilution indicator in the stomach.


The surgical management of corneal oedema, with special reference to corneal grafting.


Life, death, and heart transplantations.


Preparing for kidney transplantation.

Preparing for kidney transplantation.


A modified Snyder test for caries-activity in humans.


Physicochemical characteristics of insulin secretion granules. beta-Granules were prepared from micro-dissected pancreatic islets of obese-hyperglycaemic mice. This fraction contained 60% of the insulin, 30% of the cytochrome oxidase, 16% of the acid phosphatase activity and 20% of the protein present in whole islets. The isolated granules retained a heavy metal during fractionation. Optimum conditions for granule stability were low ionic strength and pH6, the granules being unexpectedly fragile at pH7.4. The stability of the granules was unaffected by sucrose in the concentration range 50-320mm, but 1% (w/v) sodium deoxycholate released all insulin. A solubilizing effect was also noted with ATP and citrate. Spinning through 1.6m-sucrose yielded a further purification in relation to mitochondria and acid-phosphatase-carrying particles but virtually no purification in relation to protein. Electron microscopy revealed that the major contaminants were rough-surfaced vesicles and membranes. A separation of granules from acid phosphatase was achieved by phase distribution in polyethylene glycol and dextran. The location of the enzyme to the interphase was so pronounced in systems buffered with lithium phosphate that the technique may be used for future purification of acid-phosphatase-carrying particles from the beta-cells.


Acceleration of gluconeogenesis from propionate by Dl-carnitine in the rat kidney cortex. 1. The rate of gluconeogenesis from propionate in rat kidney-cortex slices was stimulated up to 3.5-fold by dl-carnitine and by bicarbonate, and was inhibited by inorganic phosphate or high concentrations of propionate (above 3mm). 2. The stimulatory effect of carnitine was dependent on the bicarbonate concentration and could be replaced at low propionate concentration by addition of 25mm-bicarbonate-carbon dioxide buffer. At low bicarbonate concentration the carnitine concentration can be rate-limiting. 3. All observations are in accordance with the view that the action of carnitine is in principle the same as that established for other fatty acids in other tissues, namely that carnitine promotes the appearance of propionyl-CoA within the mitochondrion by acting as a carrier. 4. The accelerating effects of carnitine and bicarbonate and the inhibitory effect of phosphate can be explained on the basis of the known properties of key enzymes of propionate metabolism, i.e. the reversibility of the reactions leading to the formation of methylmalonyl-CoA from propionyl-CoA. 5. 5mm-Propionate caused a five- to ten-fold fall in the free CoA content of the tissue. This fall can account for the inhibition of respiration and gluconeogenesis caused by high propionate concentration. 6. Relatively large quantities of propionyl-l-carnitine (15% of the propionate removed) were formed when dl-carnitine was present; thus the ;activation' of propionate proceeded at a faster rate than the carboxylation of propionyl-CoA. The metabolism of added propionyl-l-carnitine was accompanied by glucose synthesis. 7. The appearance of radioactivity from [2-(14)C]propionate in both glucose and carbon dioxide was as expected on account of the randomization of C-2 and C-3 of propionate, i.e. the formation of succinate as an intermediate. 8. The maximum rate of glucose synthesis from propionate (93.3+/-3.3mumoles/g. dry wt./hr.) was not affected by dietary changes aimed at varying the rate of caecal volatile fatty acid formation in the rat. 9. Inhibition of gluconeogenesis by high propionate concentration was not found in those species where the rate of caecal or ruminal propionate production is high under normal conditions (rabbit, sheep and cow).


Lung sounds.

Lung sounds.


Combined effects of chemotherapy and immunity against leukemia L1210 in DBA-2 mice.


Nucleolar fragmentation in L cells exposed to quinacrine in vitro.


Bone infarction in antiquity.

Bone infarction in antiquity.


The monoflask system.

The monoflask system.


A new electrocardiographic concept: synchronized sinoventricular conduction.


The mechanisms of action of antihypertensive drugs.


Function and morphology of the canine lung two years following immediate or delayed reimplantation.


A cardiologist's view of modern cardiovascular surgery.


Operations for coronary disease. Report of the Committee on Cardiovascular surgery. American College of Chest Physicians.


Clinical evaluation of SKF-14336 in the treatment of psychosis associated with organic brain syndrome.


Biological relationships of the pentastomida; a bibliography on the pentastomida.


Use of thiothixene in neurotic reactions.


Schizophrenia and delinquency: the inadequacy of our conceptual framework.


The Paul Weaver Chorale. A California dentist inspires a 40-voice chorus.


The odontogenic cyst as a potential carcinoma: A clinicopathologic appraisal.


A comparative study of the isolation of the cortex and the role of the calcium-insoluble protein in several species of sea urchin egg. A comparative study was made of the isolation of the cortex in the eggs of several sea urchin species. Since the isolation method developed by Sakai depends on the presence of magnesium in the medium, the protein composition of the cortex was investigated to determine whether the protein component of the egg described by Kane and Hersh which is gelled by divalent ions, is present in these cortices. Isolation of the cortex was found to require the same divalent ions at the same concentrations as protein gelation, and in the eggs of some species much of the gel protein of the cell was found in the isolated cortical material. In the eggs of other species a smaller fraction of this protein was found in the isolated cortex, although it was more concentrated there than in the endoplasm, and in one species this protein appeared to be uniformly distributed throughout the cell. These results indicate that this protein is localized in the cortical region of the eggs of some species of sea urchin, possibly in the cortical granules, but also point up the fact that results from one species cannot be uncritically extrapolated to others.


Effects of division-synchronizing hypoxic and hyperthermic shocks upon Tetrahymena, a respiration and intracellular ATP concentration. The division of Tetrahymena pyriformis GL cells was synchronized with either seven hypoxic or five hyperthermic (heat) shocks. Hyperthermic shocks of 34 degrees C produced no reduction in respiration rate and only a 19% decline in intracellular ATP concentration. Hypoxic shocks of 0.15% ambient oxygen concentration depressed intracellular ATP concentration 50%. It therefore appears that hypoxic shock, but not hyperthermic shock, reverses progress of Tetrahymena toward fission by reducing ATP concentration through a reduction of the rate of oxidative phosphorylation. After the first synchronized division, whether synchronized by intermittent hypoxia or hyperthermia, total respiration rate increased exponentially at the same rate of increase as total respiration rate in an exponentially growing (log phase) Tetrahymena cell culture. Before the first synchronized division, the total respiration rate increased exponentially but more slowly than after completion of the first synchronized division. The pattern of increase of total respiration during division synchronized by either procedure was different than the pattern of increase of total respiration of synchronous cells observed by Zeuthen.


Isolation and properties of secretory granules from rat islets of Langerhans. I. Isolation of a secretory granule fraction. A method has been devised for the isolation of a secretory granule fraction from isolated rat islets of Langerhans. The islets were homogenized in buffered sucrose, and the homogenate was separated into nuclear, mitochondrial, secretory granule, and microsomal fractions by differential centrifugation. The secretory granule fraction was purified by differential centrifugation in discontinuous sucrose density gradients. A greater degree of purification could be achieved by the use of two successive gradients of this type, although the final yield was greatly reduced. Biochemical and morphological characterization of the fractions was obtained; the secretory granule fraction contained both insulin and glucagon. The limiting membranes of the granules remained intact and the general appearance of the granules was similar to that seen within the whole islet cells.


Isolation and properties of secretory granules from rat islets of Langerhans. II. Ultrastructure of the beta granule. Beta granules isolated from rat islets of Langerhans and subjected only to phosphotungstic acid had, in negatively stained images, a 50-A periodicity. This periodicity was also observed in thin-section profiles of beta granules in intact cells. In shadowed preparations, the granules were spherical in shape and had irregular edges and surface structure. The presence of such a periodicity in the beta granule indicates that its matrix may be composed of a crystalline material.


Isolation and properties of secretory granules from rat islets of Langerhans. 3. Studies of the stability of the isolated beta granules. A partially purified secretory granule fraction, isolated from rat islets of Langerhans by differential centrifugation, was used for investigating the stability of the beta granules during incubation in various conditions. Effects of pH, temperature, and time were studied; the granules possessed optimal stability at 4 degrees and pH 6.0, and could be solubilized at pH 4.0 or 8.5, or in the presence of sodium deoxycholate, but not by phospholipase c, ouabain, or alloxan. Incubation with glucose or some of its metabolites, or with tolbutamide, ATP, or cyclic 3',5'-AMP did not alter the stability of the beta granules Exogenous insulin-(131)I was not bound by the isolated granules under the conditions used; no specific insulin-degrading activity could be detected in subcellular fractions of the islets. These findings indicate that intracellular solubilization of the granules with subsequent diffusion of the insulin into the extracellular space is not a likely mode of insulin secretion in vivo, and suggest that a crystalline zinc-insulin complex may exist in the matrix of the beta granules.


A study of nexuses in visceral smooth muscle. Nexuses are described between the smooth muscle cells of the gizzard of the chick and the pigeon, the vas deferens of the mouse and the guinea pig, and the taenia coli of the guinea pig. The nexuses in the gizzard were examined after osmium tetroxide and potassium permanganate had been used as fixatives. Although differences in the dimensions of the unit membranes and the nexuses were noted, the results with the two fixation techniques were complementary. The distribution of nexuses within the smooth muscle tissues examined was uneven. Nexuses were still present in both small and large pieces of tissue incubated in hypertonic solutions at varying temperatures. Other experiments showed that the degree of contraction at the time of fixation did not affect the presence of nexuses in the tissue. These results indicate that nexuses between smooth muscle cells are stable under a variety of conditions.


The rapid titration of Semliki forest virus in cell monolayers by immunofluorescence.


Nucleolar orthophosphate ions. Electron microscope and diffraction studies. Lead acetate (3-10%, pH between 4.3 and 7.0, alone or containing 2% glutaraldehyde), when used as fixative, has been demonstrated to produce an intracellular microcrystalline precipitate of lead orthophosphate, Pb(5)(PO(4))(3)OH (lead hydroxyapatite). This confirms earlier work with the light microscope (6). In interphase cells the nucleoli are sharply delimited by the massive lead phosphate precipitate. Some diffuse precipitate is found in the nucleoplasm; it is always delimited by the nuclear membrane. Nucleolar localization of this orthophosphate pool is not a diffusion artifact; the pool is probably in a loosely bound state and is not retained by conventional fixatives. In maize root cells in advanced mitotic stages the lead phosphate crystals are seen distributed throughout the cytoplasm and also relatively concentrated on the late anaphase-early telophase chromosomes. This pool of inorganic phosphate anions may be involved in the mitotic cycle of chromatin condensation, and it may be partially responsible for the absence of mature ribosomes in the nucleolus through the chelation of divalent cations. It is evident that the siver-reducing component detected in the nucleoli of fixed cells (6) is a completely different substance.


Induction of a defective phage and DNA methylation in Escherichia coli 15T-.


Transformation of rat embryo cells by adenovirus type 1.


Antigenic differences between wild type and mutants of bacteriophage lambda.


Growth of tumors in allogeneic hosts after organ culture explantation. II. Tumor-host interactions.


Biological effects of the neonatal injection of 7,12-dimethylbenz[a]anthracene.


Secondary disease in irradiated mice grafted with allogeneic bone marrow from antilymphocyte serum-treated donors.


Museum notes.

Museum notes.


Resynthesis of growth hormone releasing factor in the rat hypothalamus after insulin hypoglycemia.


Autoradiography may be unreliable for identifying human chromosomes.


Mast cells, heparin, and osteoporosis.

Mast cells, heparin, and osteoporosis.


Atheroembolic renal disease.

Atheroembolic renal disease.


Gastritis.

Gastritis.


New drugs: the tortuous road to approval.


Relationship of life change maladaptive aggression, and upper respiratory infection in male college students.


Pulmonary oxygen toxicity at one ATA.

Pulmonary oxygen toxicity at one ATA.


Double-blind experimentation in anaesthesiology.


Effect of intercostal block on lung function after thoracotomy.


Genetics of epilepsy: a review.

Genetics of epilepsy: a review.


Simultaneous measurements of hepatic and portal venous blood flow in the sheep and dog.


Direct bacterial count as a rapid freshness test for fish fillets. Comparison of various indices of deterioration of refrigerated fish fillets showed that the direct bacterial count can be used to predict the storage life of the foodstuff. For direct counts, a thin film made from fillet surface material was spread on a microscope slide, stained, and read. Initial counts were found to correlate well with keeping quality; a period of freshness of 24 or 48 hr at 5 C could be reliably predicted. Preliminary data indicated that hypoxanthine estimation could probably also be used for the prediction of shelf life but that the relative complexity of the procedure detracted from its usefulness.


Potentiation of acid secretion in dogs.

Potentiation of acid secretion in dogs.


Effect of shaking speed and type of closure on shake flask cultures. Growth of microorganisms and biosynthesis of microbial products in shake flasks may be limited by operating conditions which provide inadequate supplies of oxygen. Methods are described for meeting the oxygen requirements of test organisms by using standard baffled flasks with pad-type closures and shaking at relatively high speeds. Growth of Escherichia coli B in a rich medium and production of candidin by Streptomyces viridoflavus were the test systems used. Flasks shaken at 230 to 385 rev/min gave sulfite oxidation rates of 1 to 8 mmoles of oxygen per liter per min over a useful working volume range (40 to 150 ml in 300-ml flasks). These rates are as high as those obtained in agitated fermentors under usual operating conditions.


Frequency of R factor-mediated multiple drug resistance in Klebsiella and Aerobacter. A comparative study was done on the transfer frequency of R factors from 90 strains of multiple drug-resistant Aerobacter and 81 strains of Klebsiella to Escherichia coli CSH-2 (F(-), met(-), pro(-), Nal-r). The most common resistance patterns for the Aerobacter isolants were ampicillin streptomycin chloramphenicol tetracycline and ampicillin streptomycin chloramphenicol tetracycline kanamycin neomycin; for the Klebsiella isolants, the most common resistance pattern was ampicillin kanamycin streptomycin tetracycline chloramphenicol neomycin. R factors were isolated from 14.1% of the Aerobacter strains; 61.5% of these R factors harbored R determinants for ampicillin streptomycin tetracycline. R factors were isolated from 79.1% of the Klebsiella strains; four R factors were isolated with significant frequency; streptomycin chloramphenicol kanamycin neomycin, 37.5%; ampicillin streptomycin tetracycline kanamycin neomycin, 14.1%; ampicillin streptomycin tetracycline, 12.5%; and streptomycin chloramphenicol tetracycline, 12.5%.Chloramphenicol, kanamycin, and neomycin resistance was rarely transferred from the Aerobacter strains, although over 50% of the clinical isolants possessed resistance to these antibiotics. In contrast, over 75% of the Klebsiella strains transferred resistance to chloramphenicol, kanamycin, neomycin. Highest frequency of transferred resistance to individual drugs in the Aerobacter strains was to streptomycin (14.8%), whereas in the Klebsiella group resistance to four drugs was transferred at a very high frequency: streptomycin (80.8%), chloramphenicol (78.5%), kanamycin (76.4%), and neomycin (75.9%).


Technique for the aseptic addition of liquid to flask cultures.


Packaging method for preserving agar plates during prolonged incubation.


Antibiotic assaying by use of a direct plate technique.


Room temperature storage of Treponema pallidum on microscope slides for use in the FTA-ABS test.


Improved agar gradient-plate technique.

Improved agar gradient-plate technique.


Acute cholecystitis in a municipal hospital. The role and results of cholecystostomy.


Homograft skin for wound coverage. A study of versatility.


The Peutz-Jeghers syndrome. Is there a predisposition to the development of intestinal malignancy?


Genetic and population studies of quantitative levels of adenosine triphosphate in human erythrocytes.


Infrared spectra of a few transfer ribonucleic acids.


Corticosteroid therapy for lymphomas and chronic lymphocytic leukemia.


Diverticular disease of the colon: a 1968 view.


Primary lymphadenopathy complicating idiopathic steatorrhoea.


How far to go with Enterobacteriaceae?

How far to go with Enterobacteriaceae?


Fatal falciparum malaria in the United States.


Clinical evaluation of three long-acting anticholinergic compounds.


Bristol Award.

Bristol Award.


Cephaloglycin: crossover absorption studies and clinical evaluation.


Effects of structural modifications of catecholamines on renal tubular transport in the chicken.


Urinary tract infection and renal homotransplantation. I. Effect of antibacterial irrigations on defenses of the defunctionalized bladder.


Management of a thermal burn with amputation and reconstruction of the penis.


A comparison of the behavioral effects of periodic administration of chlorpromazine and a "sustained-release" form of chlorpromazine.


Intrarenal distribution of blood flow in canine renal allografts treated with immunosuppressive drugs.


Serratia marcescens infection of the urinary tract: a nosocomial infection.


Comparison of clorexolone, a new diuretic, with hydrochlorothiazide.


Development of the present concept of hemophilia.


Routine rubella antibody titer determinations in pregnancy.


Simple removal of cerclage suture for incompetent cervix.


Urethrovesicovaginal fistula.

Urethrovesicovaginal fistula.


Toward prophylaxis of prenatal infection by viruses.


Premature cervical dilatation and the McDonald cerclage.


Hypernatremia following intrauterine instillation of hypertonic saline solution. Report of a case and discussion.


Placement of spinal cord injury patients in community nursing homes at VA expense.


History and program of California PVA.

History and program of California PVA.


The effect of oral zinc sulfate on the healing of decubitus ulcers in spinal cord injured patients.


Surgical management of the patient with classical hemophilia.


The effect of artificial respiration on the regional distribution of ventilation examined with xenon-133.


A graphical analysis of the respiratory mechanics during I.P.P.R. and I.P.N.P.R.


Effect of local anaesthetics and vasoconstrictors upon regional blood flow.


Synthetic pituitary posterior-lobe hormones as vasoconstrictors in local anaesthesia.


Marcaine in oral surgery. A clinical comparative study with carbocaine.


Measurements of concentrations of halothane with Gardner Universal Vaporisers.


Phenoperidine in anaesthesia of respiratory cripples.


Phenoperidine in respiratory insufficiency.


Blood flow through lung and skeletal muscle in connection with anesthesia and controlled respiration.


The elimination of the ventilator as a source of cross infection during intermittent positive-pressure respiration.


The cross-sectional outlines of human enamel prisms.


The reaction of the reticulolymphocytic system and humoral IgM-antibody in skin-homograft rejection.


Treatment of hepatic coma.

Treatment of hepatic coma.


A tract for the times: some sociobiologic aspects of science, race, and racism.


Structure and development of the asbestos body.


Multiple molecular forms of the alkaline phosphatase of Escherichia coli.


Genetic and biochemical studies of enzymatically active subunits of E. coli tryptophan synthetase.


Structure and evolution of triose phosphate and lactate dehydrogenases.


The role of multiple enzymes in the regulation of branched metabolic pathways.


Boris V. Morkovin (1882-1968).

Boris V. Morkovin (1882-1968).


The molecular lesion of saccharose intolerance.


Disaccharide malabsorption.

Disaccharide malabsorption.


Malignancy and steatorrhea.

Malignancy and steatorrhea.


Familial chloride diarrhea-chloride malabsorption.


Some effects of extensive intestinal resection in childhood.


Intestinal hydrolysis of starch.

Intestinal hydrolysis of starch.


Digestion and absorption of sugars.

Digestion and absorption of sugars.


Glucose-galactose malabsorption.

Glucose-galactose malabsorption.


Studies with the RNA polymerase. I. Factors affecting the binding of nucleic acid polymers to the enzyme.


Chromatography of nucleic acids on hydroxyapatite. I. Chromatography of native DNA.


Regulation of amino acid-specific transfer RNA's in Escherichia coli.


Ribosomes of Acetabularia mediterranea.

Ribosomes of Acetabularia mediterranea.


Reutilization of ribosomal proteins in vivo for the formation of new ribosomal particles in Escherichia coli B.


Properties of ribonucleoprotein particles in chloramphenicol-treated cells of Escherichia coli B.


The chemical synthesis and coding properties of adenylyl-adenylyl-guanosine and guanylyl-adenylyl-adenosine having possible combinations of 2'-5' and 3'-5' internucleotide linkages.


Protein synthesis directed by ribopolynucleotide containing formycin.


Concerning the interrelationship of nuclear and cytoplasmic RNA's from animal cells as revealed by competition hybridization experiments.


The comparative study on the effects of chloramphenicol, erythromycin and lincomycin on polylysine synthesis in an Escherichia coli cell-free system.


Toxicity of anti-dog lymphocyte serum to normal and leukemic human lymphocytes.


Erythropoietin and erythropoiesis in anemic man following androgens.


Harvey Cushing: great pioneer.

Harvey Cushing: great pioneer.


Weight-reducing effect of diguanides in obese non-diabetic women. A double-blind trial was carried out to assess the weight-reducing effect of the diguanides in 90 women with refractory obesity and normal oral glucose tolerance. The daily dosage of phenformin and metformin was increased at weekly intervals up to 300 mg. and 3 g. respectively, and patients were maintained at this dose or on the maximum they could tolerate without anorexia or other gastrointestinal side-effects. Seventy-seven completed the 16-week period of study. There was a statistically significant difference between the mean weight change of the control and the treated groups, but no difference between those treated by phenformin or metformin. The weight-losing influence of the diguanides appeared to be no longer significant after the twelfth week of treatment.


Specific antibodies and renal transplantation.


Of voles and men.

Of voles and men.


Prilocaine-felypressin: a new combination for dental analgesia.


Brain tumor and the Tree-test.

Brain tumor and the Tree-test.


Moravia's modern Oedipus.

Moravia's modern Oedipus.


Comparison of dosage ranges of carphenazine and trifluoperazine in elderly chronic schizophrenics.


Clinical trials with thiothixene.

Clinical trials with thiothixene.


N-Terminal fragment of hog pepsin.

N-Terminal fragment of hog pepsin.


Fatty acid degradation in Escherichia coli. An inducible acyl-CoA synthetase, the mapping of old-mutations, and the isolation of regulatory mutants.


The discovery of the transference: towards an epistemological interpretation.


Lens incorporated germicides.

Lens incorporated germicides.


Social desirability in children: an extension and replication.


Mediastinal cyst with spontaneous resorption.


Need for approval and popularity: sex differences in elementary school students.


Submicroscopic structure of single neurons isolated from rabbit lateral vestibular nucleus.


Ultrastructure of calcium deposits in arteriosclerotic human aortas.


Florey memorial postdoctoral fellowships.


Preventing the rejection response to transplanted organs.


In memoriam. Leonard S. Fosdick, 1903-1969. President of IADR, 1951-1952.


Organ transplants.

Organ transplants.


Changing concepts in transplantation.

Changing concepts in transplantation.


Current status of human gonadotropin radioimmunoassay.


Study of cornification of oral epithelium by phase microscopy.


Richard Benedict Goldschmidt, April 12, 1878-April 24, 1958.


The autonomic nervous system.

The autonomic nervous system.


Radiology.

Radiology.


Surgery of the spinal cord and spinal column.


Cerebral trauma and traumatic infection of the central nervous system.


Psychiatry and law.

Psychiatry and law.


Vascular disorders.

Vascular disorders.


Criminal psychopathology.

Criminal psychopathology.


Alcoholism.

Alcoholism.


Psychosomatic medicine.

Psychosomatic medicine.


Regional physiology of the nervous system.


Psychoanalysis.

Psychoanalysis.


Group psychotherapy.

Group psychotherapy.


Psychiatric nursing and occupational therapy.


Mother and father schemata of achievers and underachievers in primary school arithmetic.


Age differences in peer ratings of socially desirable and socially undesirable behavior.


A survey of bowel preparation for barium enema patients.


Shakespeare and orthopedics.

Shakespeare and orthopedics.


The relation between serum calcium and tumor metastases.


Introduction of a mutator gene from Escherichia coli K12 into Escherichia coli C.


A new assay of the phosphotransferase system in Escherichia coli.


The non-linear relationship between the enzyme activity and structural protein concentration of thiogalactoside transacetylase of E. coli.


A mutant of Escherichia coli defective in phosphatidic acid synthesis.


The use of 131I labelled human serum albumin in the assessment of improved lymph flow following buried dermis flap operation in cases of post-mastectomy lymphoedema of the arm.


Single-plate screening test for potential radiosensitisers of hypoxic bacteria.


The search for a vaccine for syphilis. An epidemiological approach.


Serological tests for treponemal infection in leprosy patients. An evaluation of the fluorescent treponemal antibody absorption (FTA--ABS) test.


Evaluation of darkfield and immunofluorescent techniques for demonstrating Treponema pallidum in fluids with small numbers of organisms.


Simultaneous isolation of Trichomonas vaginalis and collection of vaginal exudate.


Isolation of TRIC agents from the human genital tract.


The first venereologists.

The first venereologists.


Fluorescent antibody methods in the detection and control of venereal diseases. A bibliographical review of the literature.


Psychotropic drugs in children. Pharmacotherapy as an adjunct to counseling or psychotherapy.


Immunologic deficiency states. Basic review of mechanisms of immunity.


Medicine in 1695: Friederich Hoffmann's Fundamenta Medicinae.


Actin-like filaments and cell movements in human ascites tumour cells: an ultrastructural and cinemicrographic study.


Asphalt-coloured spots of isolated functional heart muscle cells in culture.


Edgar Altenburg. January 11, 1888-August 27, 1967.


Liberty, laissez-faire and licensure in nineteenth century Britain.


The rise and decline of the altitude therapy of tuberculosis.


Felix Bernstein. 1878-1956.

Felix Bernstein. 1878-1956.


Harriett Ephrussi-Taylor. April 10, 1918-March 30, 1968.


Malpractice suits in American medicine before the Civil War.


John W. Gowen. September 5, 1893-September 14, 1967.


Gregory Goodwin Pincus.

Gregory Goodwin Pincus.


Edmund Ware Sinnott. 1888-1967.

Edmund Ware Sinnott. 1888-1967.


Directory.

Directory.


Antibiotics literature index--a select bibliography.


Study of a microbial aminopeptidase from Aspergillus oryzae determination of the N-terminal sequences of native proteins and of peptides.


Stilling's nuclei--turning point in basic neurology.


Frozen section diagnosis and mucin production in prostatic carcinoma.


Enhancement of the bladder defense mechanism by immunization.


Urine sterilization in catheter drainage bottles.


Jacob Henle (1809-1885).

Jacob Henle (1809-1885).


Alteration of physical, chemical, and biological properties of endotoxin by treatment with mild alkali. Treatment with alkali is one of several methods for removing fatty acids from bacterial endotoxins and, in the process, detoxifying the material. Saponification of fatty acid esters is the major detectable chemical change produced by alkali; however, kinetic studies of mild alkaline hydrolysis of endotoxin failed to correlate rates of detoxification with rates of loss of ketodeoxyoctonates, heptose, O-acetyl groups, or fatty acid esters. The alterations occurring during the critical stages of hydrolysis apparently changed the essential chemical conformation of endotoxic particles before cleavage of a significant amount of material took place. The rates of both saponification and detoxification were markedly increased by carrying out the reaction in media of ethyl alcohol or dimethylsulfoxide instead of water.


Nitrogen fixation by Rhodospirillum rubrum grown in nitrogen-limited continuous culture. Cell-free extracts of the photosynthetic bacterium Rhodospirillum rubrum were inconsistent in reducing N(2). An internally illuminated fermentor, designed for the continuous culture of this organism on N(2) under nitrogen-limited conditions, produced cells which yielded cell extracts with consistent activity for cell-free N(2) fixation. A nitrogen-limited continuous culture, supplied ammonia rather than N(2), gave cell-free extracts with even more active N(2) fixation. Extracts of cells grown in the fermentor with glutamate nitrogen as the limiting nutrient in continuous culture did not reduce N(2), but whole cells fixed (15)N-enriched N(2). The discovery that cells from ammonia and glutamate nitrogen-limited continuous cultures are capable of N(2) reduction suggests that R. rubrum cells produce the N(2)-reducing enzymes in response to conditions of nitrogen deficiency rather than in response to the presence of N(2). Examination of the effect of the pN(2) on N(2) reduction by cell-free preparations of R. rubrum indicated that the K(N(2)) is approximately 0.071 atm. Cell-free extracts from R. rubrum were tested for their ability to reduce substrates other than N(2).


Corepressor system for catabolite repression of the lac operon in Escherichia coli. Acetylated amino sugars, normally used in the biosynthesis of cell walls and cell membranes, were found to play a role as corepressors for catabolite repression of the lac operon in Escherichia coli. This conclusion was derived from studies conducted on mutants of E. coli that were able to assimilate an exogenous source of N-acetylglucosamine (AcGN) but were unable to dissimilate or grow on this compound. At concentrations less than 10(-4)m, AcGN caused severe catabolite repression of beta-galactosidase synthesis in cultures grown under either nonrepressed or partially repressed conditions. This repression occurred in the absence of any effect of AcGN on either the carbon and energy metabolism or the growth of the organism. In addition, this repression by AcGN occurred in a mutant strain that is constitutive for beta-galactosidase production, demonstrating that the AcGN effect does not involve the uptake of inducer. This model for the corepressor system of catabolite repression is discussed in relation to the existing theories on repression of the lac operon.


Inhibition of the peptide bond synthesizing cycle by chloramphenicol. To study the mechanism by which chloramphenicol inhibits bacterial protein synthesis, we examined the kinetics of the puromycin-induced release of peptides from transfer ribonucleic acid (tRNA) in the presence and in the absence of chloramphenicol. Washed Escherichia coli ribosomes with nascent peptides which had been radioactively labeled in vivo were used for this study. When such ribosomes were incubated in the presence of 10 mug of puromycin per ml, approximately one-fourth of the radioactive peptide material was rapidly released from tRNA. This rapid, puromycin-dependent reaction is assumed to be equivalent to the peptidyl transferase reaction. Chloramphenicol inhibited the extent of the puromycin-induced release of peptides by only 50%, demonstrating that some of the peptide chains which are present on active ribosomes react with puromycin, even in the presence of chloramphenicol. The addition of the supernatant fraction and guanosine triphosphate (GTP) increased the extent of the puromycin-induced release; this additional release was completely inhibited by chloramphenicol. Peptidyl chains on washed ribosomes prepared from chloramphenicol-inhibited cells were not released by puromycin in the presence of chloramphenicol and reacted slowly with puromycin in the absence of chloramphenicol. The release of peptidyl groups from these ribosomes became largely insensitive to chloramphenicol after preincubation of the ribosomes with GTP and the supernatant fraction. We conclude that chloramphenicol does not inhibit the peptidyl transferase reaction as measured by the puromycin-induced release of peptides from tRNA, but rather inhibits some step in the peptide synthesis cycle prior to this reaction.


Genetic analysis of the glutamate permease in Escherichia coli K-12. The glutamate permeation system in Escherichia coli K-12 consists of three genes: gltC, gltS, and gltR. The genes gltC and gltS are very closely linked, and are located between the pyrE and tna loci, in the following order: tna, gltC, gltS, pyrE; gltR is located near the metA gene. The three glt genes constitute a regulatory system in which gltR is the regulator gene responsible for the formation of repressor, gltS is the structural gene of the glutamate permease, and gltC is most probably the operator locus. The synthesis of glutamate permease is partially repressed in wild-type K-12 strains, resulting in the inability of these strains to utilize glutamate as the sole source of carbon. Derepression due to mutation at the gltC locus enables growth on glutamate as a carbon source both at 30 C and at 42 C. Temperature-sensitive gltR mutants capable of utilizing glutamate for growth at 42 C but not at 30 C were found to be derepressed for glutamate permease when grown at 42 C and partially repressed (wild-type phenotype) upon growth at 30 C. These mutants produce an altered thermolabile repressor which can be inactivated by mild heat treatment (10 min at 44 C) in the absence of growth.


Some properties of excision-defective recombination-deficient mutants of Escherichia coli K-12. Strains of Escherichia coli that carry the mutation uvrA6 show no measurable excision of pyrimidine dimers and are easily killed by ultraviolet (UV) light, whereas strains that carry recA13 are defective in genetic recombination and are also UV-sensitive. An Hfr strain carrying uvrA6 was crossed with an F(-) strain carrying recA13. Among the recombinants identified, one carrying uvrA recA proved to be of exceptional sensitivity to UV light. It is estimated from the UV dose (0.2 erg/mm(2) at 253.7 nm) required to reduce the number of colony-forming cells by one natural logarithm that about 1.3 pyrimidine dimers were formed in a genome of 5 x 10(6) base pairs for each lethal event. This double mutant is 40 times more UV-sensitive than the excision-defective strain carrying uvrA6. The replication of one pyrimidine dimer is generally a lethal event in strains carrying recA13. Spontaneous breakdown and UV-induced breakdown of the deoxyribonucleic acid (DNA) of cells of the various genotypes were estimated by growing the cells in medium containing (3)H-thymidine and measuring both acid-precipitable and acid-soluble radioactivity. The UV-induced degradation in strains with recA13 did not require the uvr(+) genes and hence appears to depend upon a mechanism other than dimer excision. The greater level of survival after irradiation in Rec(+) as compared to Rec(-) bacteria may be due to a recovery mechanism involving the reconstruction of the bacterial chromosome through genetic exchanges which occur between the newly replicated sister duplexes and which effectively circumvent the damaged bases remaining in the DNA.


Replication of the bacterial chromosome: location of new initiation sites after irradiation. New loci of replication along the bacterial chromosome are observed after irradiation of Escherichia coli. It was conjectured that, after X-irradiation, the new initiation site was random with respect to the fixed-origin, whereas, after ultraviolet light exposure, it was selective and appeared to be from the fixed-origin. Evidence presented here shows that, after X-irradiation of E. coli, the new initiation site(s) for the onset of deoxyribonucleic acid replication is induced at chromosomal regions not restricted to the fixed-origin. After ultraviolet light exposure, the new initiation site is preferentially from the fixed-origin. In these studies amino acid starvation was used to synchronize chromosome replication and to allow for differential radioisotopic labeling of the chromosomal origin and terminus. To facilitate interpretation, growing cells actively replicating their chromosome were compared with cells lacking growth points at the time of irradiation. The role of these new replication sites in the observed kinetics of deoxyribonucleic acid replication following X-ray or ultraviolet light exposure is discussed.


Two forms of D-glycerate kinase in Escherichia coli. Escherichia coli K-12 synthesizes two chromatographically distinct forms of glycerate kinase which differ both in their thermolability and in the dependence of their activity upon pH. One enzymatic form, GK I, is found in cells grown with glycerate, glucarate, or glycolate. Of these compounds, glycolate is the only carbon source that elicits the synthesis of the second enzymatic form, GK II.


Regulator gene controlling enzymes concerned in tyrosine biosynthesis in Escherichia coli. Mutants of Escherichia coli K-12 have been isolated in which several enzymes concerned with tyrosine biosynthesis are derepressed. These mutants were obtained from a parent strain possessing only a single 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase isoenzyme, DAHP synthetase (tyr), by selecting for resistance to the tyrosine analogue, 4-aminophenylalanine. The mutation responsible for this derepression has been mapped and the gene, which is not closely linked to aroF and tyrA, has been designated tyrR.


Inhibition of 3-deoxy-d-arabinoheptulosonic acid-7-phosphate synthetase (trp) in Escherichia coli. The growth of a strain of Escherichia coli K-12 which possesses only the single isoenzyme 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase (trp), and which makes this enzyme constitutively, is inhibited by tryptophan. The accumulation of DAHP by a derivative of this strain unable to convert DAHP to dehydroquinate is also inhibited by tryptophan. The enzymic activity of DAHP synthetase (trp) in the presence of CO(2+) is sensitive to inhibition by tryptophan. Mutant strains of E. coli have been isolated in which DAHP synthetase (trp) is feedback-resistant.


Resistance factor-mediated streptomycin resistance. Resistance (R) factor-mediated streptomycin (Sm) resistance differs from classical, high-level, chromosome-borne Sm resistance in its dominance over sensitivity and in the level of its effectiveness (in Escherichia coli approximately 25 mug/ml versus >2,000 mug/ml). In addition, an R factor-containing strain, unlike high-level Sm-resistant bacteria, showed an inoculum effect with respect to its level of Sm resistance. Crude extracts of this strain destroyed the inhibitory activity of Sm and bluensomycin (Blue) on in vitro protein synthesis. The ribosomes from this strain proved to be sensitive to Sm in vitro. The requirements for in vitro inactivation of Sm (and Blue) were determined to be: extract, adenosine triphosphate or deoxyadenosinetriphosphate, and Mg(++). Chromatographic techniques with radioisotopes revealed the formation of an inactivated form of Sm containing adenosine (or deoxyadenosine), phosphate, and Sm in equimolar amounts. The adenylate moiety is coupled to the streptobiosamine residue, rather than to the streptidine ring, of the Sm molecule. The adenylating enzyme, which is not induced by Sm, is located in the periplasmic space of the R factor-containing strain.


Regulation of branched-chain amino acid biosynthesis in Salmonella typhimurium: isolation of regulatory mutants. 5',5',5'-Trifluoro-dl-leucine inhibited the activity of alpha-isopropylmalate synthetase (the initial enzyme unique to leucine biosynthesis) as well as the growth of Salmonella typhimurium. Mutants of S. typhimurium resistant to the analogue were isolated and characterized. In most cases, they overproduced and excreted leucine or leucine, valine, and isoleucine as a result of an alteration in the regulation of branched-chain amino acid biosynthesis. Biochemical and genetic tests allowed the mutants to be grouped into three classes: I, a moderately large group (13%) which had high, constitutive leucine biosynthetic enzyme levels and mutant sites linked to the leucine operon (operator constitutive); II, a single mutant in which the mutant site was linked to the leucine operon and in which alpha-isopropylmalate synthetase was not inhibited by leucine (feedback negative); III, a majority type which had constitutive levels of leucine, valine, and isoleucine biosynthetic enzymes and mutant sites unlinked to the leucine operon. Mutants of class I provide important evidence for the concept of an operon organization of genes involved in leucine biosynthesis. The properties of class III mutants indicate that there is some element involved in regulation which is common to the three pathways.


Repression of the histidine operon: effect of the first enzyme on the kinetics of repression. Kinetic studies on repression of the enzymes for histidine biosynthesis in Salmonella typhimurium showed that, upon addition of histidine to a derepressed culture, the enzymes became repressed in a temporal sequence which corresponds with the positional sequence of the genes in the histidine operon. This serial pattern of repression occurred under conditions in which the feedback site of the first enzyme for histidine biosynthesis is intact. When this site was rendered nonfunctional the pattern of repression was changed so that all of the enzymes became repressed concomitantly. These results suggest that the first enzyme for histidine biosynthesis plays a hitherto unrecognized role in control of the histidine system.


Nitrate reductase complex of Escherichia coli K-12: isolation and characterization of mutants unable to reduce nitrate. Thirty-eight mutants unable to reduce nitrate were isolated from Escherichia coli and characterized biochemically and genetically. All of the mutants exhibited reduced or insignificant levels of formate dehydrogenase, nitrate reductase, or various combinations of these activities and cytochrome b(1) under conditions which resulted in the production of high levels of these activities by the wild-type parental strains. Most of the mutants reverted readily to wild type, and all mapped within a restricted region on the chromosome linked to the tryptophan genes. It was proposed that nitrate reduction in E. coli was catalyzed exclusively by an organized complex containing formate dehydrogenase, cytochrome b(1), and nitrate reductase.


Immunochemical and enzymatic comparisons of the tryptophan synthase alpha subunits from five species of Enterobacteriaceae. The reactive surface structures of alpha subunits of tryptophan synthase from Escherichia coli, Shigella dysenteriae, Salmonella typhimurium, Aerobacter aerogenes, and Serratia marcescens were compared by measuring (i) their reactivities in micro-complement-fixation assays with antibodies directed specifically to E. coli wild-type alpha subunit, (ii) their reactivities in enzyme neutralization assays with the same antibodies, and (iii) their binding affinities for tryptophan synthase beta(2) subunits. The enzymes from the four heterologous species cross-reacted in the microcomplement-fixation assays with the anti-E. coli alpha subunit antibodies, each to a different degree. However, neutralization titers of the antibodies reacting with the various alpha subunits were comparatively similar, and the beta(2) subunit-binding and -stimulating abilities of the alpha subunits were even more closely alike. The results suggested that the tertiary structure of the beta(2) subunit-binding site of the alpha subunit has been conserved, relative to the rest of the molecule, during the evolutionary divergence of the species of Enterobacteriaceae.


Cold-sensitive mutations in Salmonella typhimurium which affect ribosome synthesis. A number of mutations (45) expressed as cold-sensitive conditional lethal pheno-types were screened by transduction for their linkage to the streptomycin-resistance locus; 7 showed such linkage. Of these, two were studied in greater detail. The sedimentation profiles of ribosomes from cultures grown at low temperature differed from wild type and from one another. Both mutants lost ribonucleic acid control at low temperature. It is suggested that a high proportion of mutants expressing a cold-sensitive phenotype harbor mutations in genes affecting ribosome synthesis or regulation.


Phosphatidic acid synthesis in Escherichia coli. The kinetic properties of acyl-coenzyme A (CoA): l-alpha-glycerol-phosphate trans-acylase (EC 2.3.1.15) from Escherichia coli were studied. At 10 C, a temperature at which the reaction was proportional to time and enzyme concentration, the enzyme had an apparent K(m) of 60 mum for l-alpha-glycerol-phosphate. The curve describing the velocity of the reaction as a function of palmitoyl-CoA concentration was sigmoid but the plot of v(-1) versus [S](-3) gave a straight line. A K(m) of about 11 mum was calculated for palmitoyl-CoA. Adenosine triphosphate specifically inhibited the reaction, being a noncompetitive inhibitor in respect to l-alpha-glycerol phosphate. Inhibition only occurred with high concentrations of palmitoyl-CoA, and maximal inhibition was 60%.


Mutant of Escherichia coli with anomalous cell division and ability to decrease episomally and chromosomally mediated resistance to ampicillin and several other antibiotics. In a mutation experiment with a rough, ampicillin-resistant strain, we isolated two smooth mutants which were both sensitive to ampicillin and carried defects in the cell envelope. One of the strains (with the envA gene) is hindered in its completion of septa and forms chains of cells. The envA gene has been mapped to a position between leu and proB, at 2 to 4 min. The envA gene decreased the resistance mediated by both episomal and chromosomal genes for resistance to several antibiotics. During growth the envA mutant was characterized by abnormal ratios between viable count or cell count and optical density. The ratio between viable count and optical density was affected during shift-up and shift-down experiments. When compared to the parent strain, the envA mutant was found to be more resistant to ultraviolet irradiation on plates. Prestarvation for tryptophan had a protective effect against irradiation both on the parent strain and the envA mutant.


Changes in the ultraviolet sensitivity of Escherichia coli during growth in batch cultures. The ultraviolet (UV) sensitivity of Escherichia coli B/r harvested at various times during growth in batch cultures was measured. The results showed a period of increased UV sensitivity in late log phase, just before the cultures entered stationary phase. This increase in sensitivity was associated with a decreased shoulder in the UV survival curves. The postirradiation division delay of survivors was shortest for cells harvested during the period of maximal sensitivity. This period of increased UV sensitivity during late log phase was not found in the radiation-sensitive, repair-deficient mutant B(s-1) (a strain which is unable to excise pyrimidine dimers from UV-damaged deoxyribonucleic acid). These results suggest that the variation in UV sensitivity of E. coli B/r as a function of time of harvesting of the cells from batch cultures is related to the varying capacities of these populations to repair UV-damaged deoxyribonucleic acid. Further experiments designed to elucidate the mechanism underlying this variation in UV sensitivity indicated that it arises from the partial depletion of nutrients in the medium during late log phase. We suggest that growth in such depleted media leads to a depression in the intercellular concentration or activity of one or more of the repair enzymes concerned with the repair of damaged deoxyribonucleic acid.


Salmonella abony-Salmonella typhimurium recombinant nonvirulent for the mouse. A previous genetic investigation involving a mouse-nonvirulent Salmonella abony donor (high frequency of recombination) and a virulent S. typhimurium recipient indicated that two unlinked "low-virulence" loci determined nonvirulence. A nonvirulent recombinant was analyzed to determine the basis for its nonvirulence. The recombinant was smooth (like the parental strains) and prototrophic. The doubling time in mouse serum of the recombinant and the S. abony parent (both streptomycin-resistant) was longer than that of the wild-type streptomycin-sensitive ancestor of the S. typhimurium recipient. The virulent recipient also grew poorly in serum. However, the nonvirulence of the recombinant was probably not due to its inheritance of the streptomycin-resistance allele from the donor, because other recombinants were streptomycin-resistant but still virulent. Unlike the nonvirulent S. abony (but like the S. typhimurium), the recombinant was insusceptible to rapid intravenous clearance in normal mice. It therefore appears that neither of the "low-virulence" loci determine diminished virulence by enhancing phagocytosis. Clearance of the recombinant was enhanced by opsonization with immune serum. Counts of viable bacteria in the blood, liver, and spleen of normal mice after intravenous challenge showed that the recombinant, like the S. abony donor, failed to proliferate in the tissues, whereas the virulent S. typhimurium did so markedly. It is concluded that the nonproliferation of the recombinant was determined by one or both of the "low-virulence" loci from the nonvirulent S. abony donor.


2-Deoxyribose gene-enzyme complex in Salmonella typhimurium: regulation of phosphodeoxyribomutase. Phosphodeoxyribomutase, the enzyme which catalyzes the interconversion of 2-deoxyribose-1-phosphate to 2-deoxyribose-5-phosphate, has been partially purified from Salmonella typhimurium. The enzyme had an absolute requirement for manganese ion and was stimulated by glucose-1, 6-diphosphate. Phosphodeoxyribomutase was induced by deoxyribose-5-phosphate and was coordinately regulated with the enzymes thymidine phosphorylase and deoxyribose-5-phosphate aldolase, type II. Mutants deficient in these three enzymes were isolated and mapped close to the threonine locus in S. typhimurium. The three enzymes thymidine phosphorylase, deoxyribose-5-phosphate aldolase, type II, and phosphodeoxyribomutase are controlled by a series of linked genes and appear to constitute an operon.


Characterization of pyridoxine auxotrophs of Escherichia coli: P1 transduction. Pyridoxine mutants of Escherichia coli B, previously divided into a minimum of six groups by cross-feeding tests, were characterized by transduction studies performed with phage P1bt. The results of these studies allowed division of pyridoxine mutants into five unlinked groups and set the minimum number of enzymes between pyridoxal phosphate and a metabolite common to other pathways at six or seven, with the probable maximum at ten. One group was shown to be linked to thr, leu, and pyrA.


Characterization of a beta-galactosidase formed between a complementary protein and a peptide synthesized de novo. In a cell-free system, phi80dlac can be transcribed, and the resulting ribonucleic acid can be translated to yield a product which interacts with an enzymatically inactive z protein to produce active enzyme. The inactive z protein is produced by Escherichia coli strain 21, which contains a deletion in the first part of the gene for beta-galactosidase and appears to exist as a dimer. The enzyme formed in the cell-free system appears to be composed of one strain 21 z protein dimer and one newly synthesized polypeptide chain with a molecular weight of about 3 x 10(4). The estimated size of this complementing segment is in good agreement with Ullmann, Jacob, and Monod's estimate of the size of the alpha region of beta-galactosidase. Using alpha fragments produced by autoclaving or guanidine treatments, we found that the active portion of alpha seems to be smaller than the full alpha region. We also found, using alpha produced by the autoclaving technique, that active dimer undergoes conversion to tetramer as the amount of alpha is increased. Evidently, the binding of alpha favors this conversion, but it is unlikely that the conversion of dimer to tetramer per se results in increased enzyme activity.


Isolation and mapping of polynucleotide phosphorylase mutants of Escherichia coli. Three strains of Escherichia coli with altered polynucleotide phosphorylase, Q7, Q13, and Q27, were isolated by screening clones from heavily mutagenized cultures for low levels of the enzyme. The three mutations were found to cotransduce with argG and asp, and the pnp locus which they define was mapped with respect to these loci. An explanation for the nonreciprocal cotransduction frequencies observed with asp is provided by the demonstration of an unlinked asp-suppressing locus.


Growth of trachoma agent in primary cultures of rabbit corneal endothelial cells. Primary cultures of rabbit corneal endothelial cells supported growth of trachoma agent, as evidenced by the production of inclusions and an increase of infectivity titers.


Electron microscopic examination of Corynebacterium ovis. Corynebacterium ovis (C. pseudotuberculosis) was examined by electron microscopy after being subjected to various methods of fixation. The organism exhibited a fine structure similar to other corynebacterial species in the appearance of its cell wall, plasma membrane, nuclear apparatus, cytoplasmic matrix, wealth and complexity of intracytoplasmic membrane systems, and polyphosphate granules. An outstanding structural feature was the existence of an electron-dense, floccular layer external to the cell wall which both ligroin and acetone-methanol extractions demonstrated to be the previously postulated surface lipid of this organism. The only variations in structure evident between virulent and attenuated strains was a quantitative difference in the thickness and appearance of the surface lipid. The observation of this layer provided a basis for explaining the surface properties of C. ovis, with particular respect to its clumping capacity in suspension, the waxiness of its growth on solid media, and its ability to grow as a pellicle on suitable liquid media. The variation in the visible amount of surface lipid between the virulent and avirulent strains adequately explained the divergence of these three surface properties between the strains.


Characterization of polynucleotide phosphorylase mutants of Escherichia coli. Three polynucleotide phosphorylase mutations, isolated in heavily mutagenized Escherichia coli strains Q7, Q13, and Q27, were characterized after their transfer by P1 transduction to nearly isogenic strains which lack ribonuclease I. Each strain has a different altered form of polynucleotide phosphorylase. One enzyme exhibited sharply reduced activity under all conditions tested. A second had reduced activity which was stimulated by Mn(++). The third enzyme was thermolabile and could be >95% inactivated in vivo at 44 C and pH 6 if the cells were prevented from growing; during growth under these and other conditions, the full enzyme level was maintained. The strains showed no differences from the wild type in their growth rates, their adjustments to changes in media and temperature, or their recoveries from starvation.


Genetic and physiological analysis of glutamate decarboxylase in Escherichia coli K-12. No correlation was found between glutamate decarboxylase (GAD) activity and the ability of Escherichia coli K-12 strains to grow on glutamate. A gene, gad, determining GAD activity maps near gltC, which controls glutamate permease.


Formation of recombinants between nontransmissible drug-resistance determinants and transfer factors. Noninfectious drug-resistance determinants acquired conjugal transmissibility by the formation of recombinants with transfer factors, suggesting the origin of R factors.


Genetic locus for ribonuclease I in Escherichia coli.


Role of erythrocytes in the acute platelet response to operation. A marked increase in platelet adhesiveness was shown to occur within four hours of operation. Postoperative red cells produced greater adhesiveness in platelets from a common pool than did preoperative red cells. A greater increase in platelet adhesiveness was observed following operation with the whole blood technique than when exogenous adenosine diphosphate was added to platelet-rich plasma. Release of adenosine diphosphate from red cells so facilitated may contribute to the acute platelet-adhesiveness response to operation.


Effect of dilution on the growth of bacteria from blood cultures. Artificial blood culture systems were set up to find the dilution with medium necessary to overcome the natural antibacterial effect of blood. The results indicate that blood should be diluted at least 1 in 50 in medium unless an additive such as Liquoid is used.


Differentiation of foetal and maternal erythrocytes in formol-fixed tissues.


Advantage of a routine Reiter protein complement-fixation test in the serodiagnosis of syphilis in pregnancy. One hundred and twenty-seven (0.3%) reactive serological tests for syphilis were obtained by routine examination of sera from 35,912 antenatal patients.Forty-eight (38%) of these reactive sera were associated with reactive fluorescent treponemal antibody and Treponema pallidum immobilization results and these patients were therefore considered to show serological evidence of treponemal infection. Sera from 13 of these 48 patients (27%) gave reactive Reiter protein complement-fixation tests in the absence of detected ;reagin' antibody. Seventy-nine patients showed non-specific biological false positive reactions to the routine tests. Following clinical assessment of these serologically reactive patients, approximately one in 100 immigrant and one in 2,500 non-immigrant pregnant women examined in this series were treated with penicillin; this represented just over half the number of patients with serological evidence of a treponemal infection, a ratio which was similar for immigrant and native born women. Yaws was the likely treponemal infection in the majority of infected immigrants. The significance of the serological results and the advantages of the Reiter protein complement-fixation test are discussed.


Mechanical rotary device for plating out bacteria on solid medium.


A new medium for the investigation of salmonella outbreaks.


Effect of Berkefeld filtration on the binding activity of human gastric juice. The intrinsic factor content of Berkefeld-filtered human gastric juice has been studied. This appears to vary with the pH at which filtration is carried out and also between individual filters. Significant losses of intrinsic factor may result from filtration and complete loss when filtration is carried out at a low pH. The most suitable pH for filtration appears to be in the range pH 7 to 8.


Pulmonary complications of oxygen therapy. Three cases of the neonatal respiratory distress syndrome are described. The babies were treated by prolonged oxygen given by intermittent positive pressure respiration after endotracheal intubation. Two of the infants died and at necropsy the lungs in both cases were consolidated. Both showed widespread haemorrhagic exudate and interstitial oedema. Early organization of the exudate was apparent in one case. The third infant survived but developed a similar exudative condition and a ;honeycomb' lung. It is suggested that many of the changes found in the lungs of these cases could be attributed to the toxicity of oxygen.


Use of dichloro-difluoromethane aerosols in preparing frozen sections.


Jack Henry Schulman (1904-1967).

Jack Henry Schulman (1904-1967).


Bound acetylcholine in the nerve ending particles.


Further studies on the fine structure of Lophomonas blattarum with special reference to the so-called calyx, axial filament, and parabasal body.


The use of glycol methacrylate for the study of the ultrastructure of virus-infected leaf cells.


Comparative fine structure study of the gametocytes of avian, reptilian, and mammalian malarial parasites.


Unity for strength.

Unity for strength.


Ear construction in children with congenital atresia and stenosis.


Baggy eyelids in young adults.

Baggy eyelids in young adults.


Split-skin grafts from hypothenar area for fingertip avulsions.


William C. Huffman, M.D. 1912-1967.

William C. Huffman, M.D. 1912-1967.


Einar Lundsgaard.

Einar Lundsgaard.


Evolution of neocortex.

Evolution of neocortex.


Multiplicity reactivation as a test for recombination function. Multiplicity reactivation of bacteriophage inactivated by ultraviolet light is dependent on the recombination function of either the host bacterial cell or the infecting bacteriophage. Absence of both recombination systems leads to a loss of multiplicity reactivation.


Serotyping for homotransplantation. 23. Analysis of kidney transplants from parental versus sibling donors.


Histocompatibility-14: correlation of the isoantigen RHO and R-Z locus.


Transplantation of allogeneic and xenogeneic (rat) marrow in irradiated mice as affected by radiation exposure rates.


Massive rabbit skin xenografts in rats.

Massive rabbit skin xenografts in rats.


Antilymphocyte serum and cell-free antigen loading.


Transfer of transplantation immunity by ribonucleic acid.


A method for the detection of antibodies in mice following graft rejection.


A comparison of the use of internal fixation and prosthetic replacement for fresh fractures of the neck of the femur.


Related base sequences in the DNA of simple and complex organisms. 3. Variability in the base sequence of the reduplicated genes for ribosomal RNA in the rabbit.


Recent advances in freeze-preservation of red blood cells.


Demonstration of phosphorylase activity in the rat brain.


Studies on reaction and binding of monoamines after fixation and processing for electron microscopy with special reference to fixation with potassium permanganate.


Joint study of extracranila arterial occlusion. 3. Progress report of controlled study of long-term survival in patients with and without operation.


Peter Redfern (1821-1912). Joint pathologist.


A historical survey of treatments for nasal polyposis.


Nevoid bassal cell carcinoma syndrome.

Nevoid bassal cell carcinoma syndrome.


Directory of otolaryngologic societies.

Directory of otolaryngologic societies.


Ketosis, weight loss, uric acid, and nitrogen balance in obese women fed single nutrients at low caloric levels.


Rate of drug metabolism in obese volunteers before and during starvation and in azotemic patients.


Neonatal tuberculosis.

Neonatal tuberculosis.


The barracks and hospital of the Cape Corps in Grahamstown in 1826.


Total body perfusion for cardiac transplantation.


Blood loss in tonsillectomy.

Blood loss in tonsillectomy.


A double-blind trial with an antidepressant drug, imipramine, in multiple sclerosis.


Facial fractures: a brief survey.

Facial fractures: a brief survey.


Experiences with doxycycline in the management of acute respiratory tract infections.


Kidney transplant and shifts in family dynamics.


T. E. Lawrence: charlatan or tragic hero?


Toward a biology of depression: some suggestions from neurophysiology.


The Lawrence enigma.

The Lawrence enigma.


The fine structure of rabbit platelets fixed in acetaldehyde.


Obesity and diabetes.

Obesity and diabetes.


What is diabetes mellitus?

What is diabetes mellitus?


Clinical experience in general practice of a new formulation of erythromycin estolate.


The effect of Dextran '70'* on post-operative deep venous thrombosis.


Unilateral electroconvulsive therapy.

Unilateral electroconvulsive therapy.


Lymphomas of small bowel and their relationship to idiopathic steatorrhea.


Porphyria variegata, a disease of kings.


Organ transplantation-today and tomorrow.


Results of operation for atrial septal defect in patients forty years of age and older.


The effect of a vasodilator cocktail, administered intra-arterially and with overpressure (for a prolonged time), on the circulation of the limb.


Reticulum cell sarcoma and lymphosarcoma. A retrospective study of potential survival in loco-regional disease.


Perfusion studies in relation to intestinal absorption.


Cell-mediated immune reaction to colon altered by bacteria.


Effect of vagotomy on the gastric secretion of acid chloride and pepsin in response to an antral stimulus and to insulin and maximal histamine stimulation.


A controlled trial of carbenoxolone sodium capsules in the treatment of duodenal ulcer.


Cochlear responses to bitonal stimuli and function of efferents.


Endodontic culturing: a statistical study.


Dental research in microbiology with emphasis on periodontal disease.


Outstanding scientists receive IADR awards.


Dr. John Shanley.

Dr. John Shanley.


The Howard University School of Nursing in historical perspective.


Post-partum psychiatric problems. A review of the literature dealing with etiological theories.


Newer perspectives in allergic disease.

Newer perspectives in allergic disease.


Dr. Leroy Randolph Weekes awarded Distinguished Service Medal for 1968.


Hyperparathyroidism.

Hyperparathyroidism.


Mabel Keaton Staupers, R.N., 1890-.

Mabel Keaton Staupers, R.N., 1890-.


Studies on dental aerobiology. I. Bacterial aerosols generated during dental procedures.


Elastomeric mold liners in the production of porosity-free polymethyl methacrylate.


Aortopulmonary window.

Aortopulmonary window.


Response to sight and sound in a simulated dental procedure.


Steroid diabetes.

Steroid diabetes.


Dostoevsky's pulmonary disease.

Dostoevsky's pulmonary disease.


Factors affecting alternate pathways of corticosteroid excretion: possible catabolite repression phenomena in man.


Connecticut gets green light on Medicare incentive pay plan.


Prof. W. J. Tulloch.

Prof. W. J. Tulloch.


A method for determining the total and differential count of Langerhan's cells in the skin.


Changes in hypothalamic responsiveness to testosterone in male Barbary doves (Streptopelia risoria).


Smallpox--and ady Mary.

Smallpox--and ady Mary.


Clinical evaluation of Babisan in 200 infants.


Lewis Webb Hill.

Lewis Webb Hill.


Oral hypoglycemic agents.

Oral hypoglycemic agents.


The pharmacology of ryanodine.

The pharmacology of ryanodine.


Drug-induced hemolytic anemia.

Drug-induced hemolytic anemia.


The respiratory mass spectrometer.

The respiratory mass spectrometer.


The influence of molecular diffusion on the dispersion of indicators in the circulation.


A comparative study of digital and analogue computer techniques for deconvolution procedures in clinical tracer studies.


The James Mackenzie Lecture. James Mackenzie and the future of medicine.


Dermatology and "The Practitioner": 1868-1968.


Chronic mental patient: current status--future directions.


The use of free transplants in restoring orbital deformities.


Metronidazole in the treatment of alcoholism. A clinical trial and review of the literature.


Research prior to the pill.

Research prior to the pill.


Strontium-90 deposition in New York City. Measurements of strontium-90 deposited in New York City over the past 12 years make for broader understanding of the fallout phenomenon. The data indicate a stratospheric half-residence time of 8 to 10 months. The seasonal oscillation of strontium-90 fallout is very symmetrical and consistent from year to year and completely independent of the timing and magnitude of nuclear tests. The predicted fallout of strontium-90 in 1970 is less than 1 percent of that during the peak year 1963.


Hu-1: Major histocompatibility locus in man. A single locus with 15 or more alleles controls reactivity in mixed leukocyte culture tests. Genes at this locus also control most of the specificities measured by cytotoxic antiserums to leukocytes. Both of these tests can be used to predict survival of skin grafts. It is proposed that this is the major histocompatibility locus in man.


Applied science and technological progress.


Neural correlates of food and water intake in the rat. Adrenergic and cholinergic stimulation of several interrelated limbic and diencephalic areas causes the animal to eat and drink, respectively. The brain areas involved may be organized in terms of "Papez circuit" and, more generally, " Nauta circuit" neuroanatomy. However, it is proposed that separate synaptic nets, one for the control of hunger and one for the control of thirst, are deployed throughout the brain at all levels, including neocortical.


Octopamine: normal occurrence in sympathetic nerves of rats. Octopamine has been identified in several organs of normal rats by means of a sensitive enzymatic assay. It is localized within the sympathetic nerve endings.


Surgery in the rat during electrical analgesia induced by focal brain stimulation. Chronic monopolar electrodes were implanted in the region of the midbrain central gray in eight rats. In three rats, continuous 60 cycle-per-second sine-wave stimulation resulted in an electrical analgesia defined by the elimination of responses to aversive stimulation while general motor responsiveness was retained. Exploratory laparotomy was carried out in these animals during continuous brain stimulation without the use of chemical anesthetics. Following surgery, brain stimulation was terminated, and responses to aversive stimuli returned. Electrodes effective in inducing electrical analgesia at the lowest currents were located at the dorsolateral perimeter of the midbrain central gray. It was concluded that focal brain stimulation in this region can induce analgesia in the absence of diffusely applied "whole brain" stimulation.


Homology and analogy.

Homology and analogy.


Functions of the lymphocytes.

Functions of the lymphocytes.


Lymphocytotoxins in sera from patients receiving renal allografts.


The treatment of septic shock: a problem intensified by advancing science.


Mechanism of actively acquired immunological tolerance.


Vitamin-related anemias. Historical survey.


Prolactin.

Prolactin.


Hemoglobin synthesis and differentiation of erythroid cells.


Mechanism and control of pyrrole synthesis.


Metabolic role of vitamin B12.

Metabolic role of vitamin B12.


Evaluation of a simple articular index for joint tenderness in rheumatoid arthritis.


Indomethacin in the treatment of rheumatoid arthritis. A controlled trial comparing indomethacin, phenylbutazone, and placebo.


Effect of Glyvenol (CIBA) on pain and on capillary resistance in rheumatoid arthritis.


Studies on the functional significance of efferent innervation in the auditory system: afferent neuronal activity as influenced by contralaterally applied sound.


Effect of intensive hyperbaric oxygen therapy on the survival of experimental skin flaps in rats.


The use of phenoxybenzamine in increasing survival in devascularized flaps.


Quantitative bacteriology and delayed wound closure.


Effects of controlled tension on the experimental burn wound.


Prolongation of xenograft survival in patients with pseudomonas sepsis: a clarification.


Effect of transplantation on age-associated characteristics of the skin.


Toxic effects of bacteria on urinary tract smooth muscle.


Effect of Escherichia coli on human spermatozoa.


Nonobstructive pyelonephritis in dogs.

Nonobstructive pyelonephritis in dogs.


The secondary healing phenomenon during lathyrism.


Alterations in lymphatic flow caused by early acute bacterial inflammation.


Antibiotic wound irrigation in the prevention of surgical wound infection.


Clinical aviation and aerospace medicine. Bethanechol chloride in the treatment of motion sickness: negative results.


A history of the education of the deaf in the Philippines.


New members and associates of the American Psychological Association.


Community psychiatry. An evolutionary change in medical psychology in the United States.


Occurrence of haemolytic Escherichia coli serogroups and their relationship to pig diseases in Queensland.


Chorismate mutase-prephenate dehydratase. Partial purification and properties of the enzyme from Salmonella typhimurium.


A general method for fractionation of nucleic acids on the basis of sequence homology.


Studies on 5-fluorouracil-containing ribonucleic acid. I. Separation and partial characterization of fluorouracil-containing transfer ribonucleic acids from Escherichia coli.


Studies with the ribonucleic acid polymerase. II. Kinetic aspects of initiation and polymerization.


Incorporation into polypeptide and charging on transfer ribonucleic acid of the amino acid analog 5',5',5'-trifluoroleucine by leucine auxotrophs of Escherichia coli.


Chemical studies on amino acid acceptor ribonucleic acids. 8. Degradation of purified alanine Escherichia coli B transfer ribonucleic acid by pancreatic ribonuclease.


Fractionation of transfer ribonucleic acid on a methylated albumin-silicic acid column. II. Changes in elution profiles following modification of transfer ribonucleic acid.


Deformylation and protein biosynthesis.

Deformylation and protein biosynthesis.


Comparison in man of lepromins prepared from leprosy infections in man and mice.


The immunoglobulin G (IgG) of Graves' disease: thyroid stimulation by fraction and fragment.


Stimulation of insulin secretion by the C-terminal tetrapeptide amide of gastrin.


Hypothalamic stimulation of follicle stimulating hormone (FSH) secretion from transplanted pituitaries following exposure to constant light.


Luteinizing hormone releasing activity in the chicken hypothalamus.


Preparation of the clinical teacher for special education: 1866-1966.


Homocytotropic antibody response in mice infected with Schistosoma mansoni: comparison with the response following Trichinella spiralis infection.


Radiotherapy for laryngeal cancer.

Radiotherapy for laryngeal cancer.


Malabsorption.

Malabsorption.


Transplantation and rejection of individual cell layers of the cornea.


The functions of the brain: Gall to Ferrier (1808-1886).


Modification of the ocular immune response. I. Use of antilymphocytic serum to prevent immune rejection of penetrating corneal homografts.


beta-Leprosol: the identification of a trialkylresorcinol from bacterial lipids.


Surveillance of haemolytic streptococcus group and type (Streptococcus pyogenes) distribution over the territory of Czechoslovakia (a five-year study).


A mutation which creates a new site for the re-initiation of polypeptide synthesis in the z gene of the lac operon of Escherichia coli.


Rate of synthesis of messenger ribonucleic acid in Escherichia coli.


The origin of the genetic code.

The origin of the genetic code.


The promoter-operator region of the lac operon of Escherichia coli.


The nature of mutants in the lac promoter region.


The internal low-efficiency promoter of the tryptophan operon of Escherichia coli.


Epidemiological aspects of neoplastic disorders in Israeli migrant population. V. The lymphomas.


Use of enzyme-labeled antibody for electron microscope localization of lymphocytic choriomeningitis virus antigens in infected cell cultures.


Marital status and figure drawing choice in normal older Americans.


Social exchange theory: an integrative approach to social conformity.


Size estimation in schizophrenics: a review and reanalysis.


Surgical education in a changing era.

Surgical education in a changing era.


Extended hemodialysis in short acting barbiturate poisoning: case report.


Cardiac output determination by digital computer: a simplified method.


On-line computer estimation of cardiac stroke volume.


A comparison of the accuracies of the electromagnetic flowmeter and the cardio-green dye dilution blood flow measurement techniques in a model.


Indicator dilution measurement of aortic insufficiency by upstream sampling in the ascending aorta.


Total interruption of the cardiac lymphatics: functional and pathologic effects.


Mechanism of action of antilymphocyte serum: effect on H-2 antibody formation, induction of allograft immunity, and tolerance to H-2 skin grafts.


The effectiveness of antisera raised against fractional antigens from murine thymocytes and lymphocytes.


Evaluation of horse anti-dog antilymphocyte globulin in the treatment of hepatic allografts.


Immunosuppression with antilymphocyte globulin in clinical renal transplantation.


Neutrophil function and infection during immunosuppression and transplantation.


Intracorporeal cadaver organ preservation by mechanical ventricular assistance.


Leucocyte group studies in renal transplant candidates.


Control of renal blood flow and sodium excretion in renal allografts by intrarenal infusion of acetylcholine.


Renin, sodium, and hypertension in patients with renal transplants.


Evaluation of heterotopic liver transplantation in dogs.


Successful twenty-four hour preservation of pancreaticoduodenal allograft with hypothermia and hyperbaric oxygen.


Prolonged survival of allogeneic guinea pig skin grafts by indomethacin.


Ventricular performance characteristics in a metabolically supported isolated heart preparation: a model for evaluating cardiac storage methods.


Immediate and early functional adequacy of transplanted lungs.


Ventilatory and hemodynamic effects of long-term lung preservation and replantation.


Changes in pulmonary vascular resistance in reimplanted dog lungs.


Dog lung allotransplantation from cadaveric donors.


Effect of homotransplantation of the dog lung on pulmonary surfactant.


Hemodynamics of the orthotopic homotransplanted heart.


Electrophysiologic observations during rejection of dog heart transplants.


Haplotyping families.

Haplotyping families.


A comparison of cytotoxins and leuko-agglutinins in human transplantation sera: implications regarding human tissue typing.


Changes in venous volume during hemorrhage: an experimental study.


In vivo measurement of the extravascular water content of the lungs in pulmonary edema.


Effect of continuous and intermittent postive pressure breathing on experimental respiratory distress.


Liver scans after orthotopic hepatic homotransplantation, biliary obstruction, and devascularization procedures.


Effect of hyperbaric oxygenation on weight gain of the liver during perfusion preservation.


An evaluation of perfusion constituents in liver preservation.


The role of afferent blood supply in regeneration of liver isografts in rats.


Inhibition of the effector mechanism of transplant immunity by local graft irradiation.


Inner table iliac bone graft.

Inner table iliac bone graft.


Ultrasonic detection of gas bubbles in blood.


The steric effect in lysogenization by bacteriophage lambda. IV. Superinfection of nonimmune lysogens.


A quarter of a century--a glance backward and forward.


An absorbable intestinal prosthesis for rapid intestinal anastomosis.


The combination of guanethidine and hydrochlorothiazide in the treatment of arterial hypertension with and without renal failure.


Microbiological evaluation of the efficacy of anti-infective drugs.


Catalytic effects of metal chelate compounds.


Amoebiasis and intestinal protozoal infections.


Malaria.

Malaria.


Maximal exercise capability of children as a function of specific cardiac defects.


Jacob Bigelow on physical diagnosis. (1839).


Comparison of a protein binding displacement method with a double isotope derivative method for determination of plasma progesterone.


Racial factors in anencephaly. A comparison of cases among American Negroes and Caucasians.


Antigenicity of trophoblastic cells indicated by fluorescein technique.


Hypothermia combined with positive pressure ventilation in resuscitation of the asphyxiated neonate. Clinical observations in 28 infants.


A bookshelf on poverty and health.

A bookshelf on poverty and health.


Beyond medicare.

Beyond medicare.


Group practice plans in governmental medical care in programs. I. Group practice prepayment plans in the medicare program.


The impact of medicare on group practice prepayment plans.


Clinical comparison of surgical wounds closed by suture and adhesive tapes.


Transplantation of the heart.

Transplantation of the heart.


Simplified technic for human auxiliary liver transplantation.


Surgical treatment of skin cancer of the nose.


The Bern Surgical Clinic revisited in memory.


Antimalarial activity of Kelfizina-trimethoprim and Kelfizina-pyrimethamine versus chloroquine in field trials in Nigeria.


Indirect fluorescent-antibody tests for parasitic diseases. V. An evaluation of a thick-smear antigen in the IFA test for malaria antibodies.


The sensitivity to Dapsone (DDS) of Mycobacterium leprae from patients with and without previous treatment.


Ocular reactions in man from deep cerebellar stimulation and lesions.


Suppression of the corneal homograft reaction by antilymphocyte serum.


Incorporation of sodium sulfate S 35 by cryopreserved corneal grafts in vivo.


John Milton McLean, MD.

John Milton McLean, MD.


An essay on the biology of osteoarthritis.


Cold-reactive rheumatoid factors in infectious mononucleosis and other diseases.


Manganese accumulation by Escherichia coli: evidence for a specific transport system.


The effect of deacylated tRNA on enzymatic binding of N-formyl-methionyl-tRNA to ribosomes.


Genetic studies of Escherichia coli K 12 mutants with alterations in glycogenesis and properties of an altered adenosine diphosphate glucose pyrophosphorylase.


Kinetics of continuous cultivation.

Kinetics of continuous cultivation.


Investigation of some methods for increasing the digestibility in vitro of microalgae.


Anticoagulant treatment in coronary heart disease.


Controlled trial of hypotensive agents in hypertension in pregnancy.


Total anomalous pulmonary venous drainage.


Haemoglobin-F. Serological conditions and occurrence in normal adults and normal pregnant and post partum women with particular reference to foeto-maternal haemorrhage.


Role of the methylated nucleic acids in carcinogenesis.


Lack of in vitro repair of x-ray-induced chain breaks in DNA by the polynucleotide-joining enzyme.


Formation of a histidine-peroxide adduct by H2O2 or ionizing radiation on histidine: chemical and microbiological properties.


Cardiac transplantation.

Cardiac transplantation.


Human heart transplantation. I. Introduction and experimental base.


Human heart transplantation. II. Clinical case data.


Human heart transplantation. 3. The operation. Donor-surgery.


Human heart transplantation. IV. Autopsy.


Human heart transplantation. V. Critical evaluation.


An experimental study of homotransplantation of tissues.


Medicolegal aspects of heart transplantation cases.


Clinical psychologists' ratings of MAPS figures along a social withdrawal scale.


Use of the family drawing as a technique for studying parent-child interaction.


Role behavior and group concept in drawings.


Self-esteem and achievement expectations for white and Negro children.


James Mackenzie and the future of medicine.


The Sheffield Congenital Anomalies Research Unit.


William Percy Blount. Veterinary scientist, consultant in poultry husbandry.


The oldest anatomical theatre.

The oldest anatomical theatre.


Percy Gore Brett.

Percy Gore Brett.


Myelin ultrastructure revealed by freeze-etching.


Douglas Arthur Warden.

Douglas Arthur Warden.


Recent studies of severe depressive illnesses. I.


Early treatment of the unconscious patient suffering from drug overdose.


Report of recovery from taipan bite.

Report of recovery from taipan bite.


The changing aspects of leukaemia.

The changing aspects of leukaemia.


Brooke Moore.

Brooke Moore.


Toxicity of disulfiram combined with metronidazole.


Treatment of angina pectoris by electrical stimulation of the carotid-sinus nerves.


The ABO and Lewis blood-group system. Immunochemistry, genetics and relation to human disease.


Self-reports by Negro and white adolescents to the draw-a-person.


Perception bibliography. LXIV. Psychological Abstracts, 1944, Volume 18.


Perception bibliography: 68. Psychological Abstracts, 1943, Volume 17.


Inter-rater reliability of scoring Goodenough-Harris drawings by disadvantaged preschool children.


Psychological differentiation and socioeconomic status.


Evaluation of Roe's theory using the hand test.


Radioresistance of bacteria as a function of p-hydroxymercuribenzoate binding.


An ultrastructural study of Mycoplasma laidlawii type A and two strains of Mycoplasma laidlawii isolated from the oviducts of dairy cows.


Skin reactions of rabbits to E. coli endotoxins and reactions in ligated intestinal loops of rabbits and piglets to live bacteria and corresponding endotoxins.


Serological diagnosis of Babesia bigemina infection in cattle by the indirect fluorescent antibody test.


Transplantaton antigens.

Transplantaton antigens.


Population policy for Americans: is the government being misled?


Dye-sensitized photooxidation of the Escherichia coli ribosome. Chemical modification of ribosomes from Escherichia coli by oxidation in the presence of selected photosensitizing dyes causes a rapid loss of their amino acid-incorporating ability. By comparing the efficiency of dyes that sensitize the photooxidation of either guanine in ribonucleic acid or amino acid residues in proteins, inactivation of one or more functional ribosomal proteins is inferred.


Aminoacyl transfer ribonucleic acid synthetases from cell-free extract of Plasmodium berghei. Aminoacyl transfer ribonucleic acid synthetases for leucine tyrosine, histidine, valine, proline, threonine, and lysine were obtainnned from cell-free extract of Plasmodium berghei. The leucyl-tRNA synthetase cane charge tRNA from liver or Escherichia coli with leucine-c(14), liver tRNA being a better substrate. The amount of aminoacylation increses linerly with respect to the quantity of tRNA added from either source and is dependent on the amount of enzyme added. The rate of aminoacylation is constant for 10 minutes and then decreases. It is enhanced by polyvinylsulfate. One-tenth millimoler pyrimethamine, hydroxystilbamidine, quinacrine, and acriflavine inhibited the formation of C(14)-valyl-tRNA. Species specificity between tRNA and its charging enzyme with respect to the recognition site is discussed.


Auditory sequence: confusion of patterns other than speech or music. Accurate perception of temporal order is essential for many auditory tasks. Yet the temporal pattern of four successive sounds (for example, hisses, buzzes, and tones) could not be recognized even when the duration of each sound was considerably longer than either the average phoneme in normal discourse or the notes of melodies. Although each of the stimuli was perceived, their order remained frustratingly elusive.


Drug safety: experimental programs.

Drug safety: experimental programs.


Modern clinico-pharmacological trends in the treatment of hypertension.


Semmelweis, Pasteur, Lister.

Semmelweis, Pasteur, Lister.


Hungary's pharmaceutical industry--a hundred-year-old.


Aminotransferase activities of branched chain amino acids in Escherichia coli.


Immunologic findings in idiopathic cardiomyopathy.


Measurement of collateral blood flow after myocardial infarction in the closed-chest dog.


Hereditary cardiomyopathy: a new disease model.


Treatment of "hypertensive encephalopathy" (accelerated hypertension). II.


Does alcohol cause duodenitis?

Does alcohol cause duodenitis?


The hemodynamic effects of changes in blood volume during intermittent positive-pressure ventilation.


Suppression of 2-deoxy-d-glucose-augmented gastric secretion by hypothalamic lesions producing hyperphagia.


Respiratory depression in the sedated bronchitic patient rapid version of the CO2 response curve.


The myocardial contractile state and its role in the response to anesthesia and surgery.


Malaria in American soldiers.

Malaria in American soldiers.


Otosclerosis (1966 and 1967).

Otosclerosis (1966 and 1967).


The Archives salutes Dr. David Cogan.

The Archives salutes Dr. David Cogan.


Twentieth anniversary of the Institute for Medical Research incorporating the Institute of Industrial Hygiene (1948-1968).


Further studies on the origin and nature of the bovine para-kappa-casein components.


Effect of steffimycin on cell-free polypeptide synthesis.


The sensitivity to rennin of an acid-soluble fraction of casein.


Studies on S-sulfo-pepsin and its cyanogen bromide hydrolysate.


Passive electrical properties of microorganisms. IV. Studies of the protoplasts of Micrococcus lysodeikticus. Observations of protoplasts of Micrococcus lysodeikticus show that removal of the cell wall of this organism decreases the dielectric constant by two orders of magnitude. The upper limit of the effective, homogeneous conductivity for the protoplast is 0.001 mho/m as compared with 0.045 mho/m for the intact cell. These results conclusively demonstrate the dominant effect of the cell wall on the low frequency dielectric properties of bacteria.


A double blind comparative trial of pericyazine and thioridazine in chronic schizophrenia.


Penicillamine and schizophrenia--a clinical trial.


Alcoholism in North-East Scotland: its treatment and outcome.


Regulation of glutamine synthetase activity in Escherichia coli.


The multienzyme alpha-keto acid dehydrogenase complexes.


Metabolism of phosphoglycerides in E. coli. 3. The presence of phospholipase A.


Proflavine and fidelity of DNA polymerase.


Death by suggestion.

Death by suggestion.


An evaluation of aversion and LSD therapy in the treatment of alcoholism.


Primary lymphocytic follicular lymphoma of liver.


Cytology and reactions of viable cells from malignant lymphoma.


The freon test. A new sensitive technic for the detection of small cardiac shunts.


Lymphatics and lymph flow.

Lymphatics and lymph flow.


R-factor gene expression gram-negative bacteria.


Demonstration of ribonuclease and desoxyribonuclease in human skin by means of fluorescent antibodies.


Characterization of a modified leucyl-tRNA of Escherichia coli after bacteriophage T2 infection.


Specially processed heterogenous bone and cartilage transplants in nasal surgery.