The medical uses of gold through the ages, valedictory address.


Uptake of glucose by islet of Langerhans and other tissues of the toadfish Opsanus tau.


Hypersensitivity and serologic responses in cattle vaccinated with disrupted Mycobacterium paratuberculosis cells and subsequently infected with Mycobacterium bovis.


A tribute (John Dutton Steele).

A tribute (John Dutton Steele).


Emergency approach to the subclavian and innominate vessels.


Julius Lempert 1890-1968.

Julius Lempert 1890-1968.


Julius Lempert. A personal appreciation.


Personal recollections of Julius Lempert.


Memories.

Memories.


Dr. Julius Lempert.

Dr. Julius Lempert.


Remarks at a memorial service for Dr. Julius Lempert.


Julius Lempert. His legacy to otology.

Julius Lempert. His legacy to otology.


Julius Lempert.

Julius Lempert.


Let us not forget the lesson of Julius Lempert.


The canal wall in tympanoplasty and mastoidectomy.


Louis Pasteur (1822 to 1895).

Louis Pasteur (1822 to 1895).


Carl Olof Nylén and the birth of the otomicroscope and microsurgery.


Directory of otolaryngological societies.


Evidence for pyrrolidone carboxylic acid in beta-galactosidase from E.


On the mechanism of the Zn2+ and Co2+-alkaline phosphatase of E. coli. Number of sites and anticooperativity.


An inhibitor  polyphenylalanine synthesis in t-RNA preparations of Saccharomyces cerevisiae.


Thermal instability of tetrahymena ribosomes: effects on protein synthesis.


Pyridyl esters of peptides as synthetic substrates of pepsin.


Template specificity of isolated chromatin.


Formation and properties of a tetrameric form of Escherichia coli alkaline phosphatase.


Effect of aminoacyl transfer ribonucleic acid on competition between guanosine 5'-triphosphate and guanosine 5'-diphosphate for binding to a polypeptide chain elongation factor from Escherichia coli.


Ribonucleotide reductases. Studies with 18-O-labeled substrates.


Analysis of deoxyribonucleic acid structure by partition chromatography.


Studies of nucleotide binding to the ribonucleic acid polymerase by a fluoresence technique.


Studies of nucleotide binding to the ribonucleic acid polymerase by equilibrium dialysis.


Escherichia coli citrate synthase. Purification and the effect of potassium on some properties.


Structural components of epithelial and endothelial basement membranes.


Inhomogeneity of vitamin K2 in Escherichia coli.


Human placental alkaline phosphatase. II. Molecular and subunit properties of the enzyme.


Subunit structure of L-asparaginase from Escherichia coli B.


The study of ionizing radiation effects on Escherichia coli by density gradient sedimentation. Density gradient sedimentation of bacterial cells in cesium chloride has been used to separate cells which have been irradiated with (60)Co gamma rays and have lost an appreciable amount of their DNA by subsequent degradation. Irradiated cells are found to band mainly at two characteristic densities, one corresponding to normal unirradiated cells and the other at a considerably lower density. The region corresponding to normal density cells is the only one that contains cells which will form colonies. Cells capable of synthesizing DNA following irradiation are found mainly at the region of normal density cells with some spreading into the lower density region. Cells in the lower density region contain less DNA than normal density cells. From an analysis of the relative numbers of cells in the two regions, it is suggested that the process of DNA degradation either takes place to a considerable extent in the genome or not at all. Analysis of the data in terms of numbers of cells having intact DNA and those having degraded DNA indicates a strong correlation between DNA degradation and cell death in this strain, JG151, and suggests that DNA degradation is a major but not the only cause of cell death.


Probes of DNA structure and interactions. Effects of copper II on ultraviolet-induced pyrimidine dimer formation. Cu(II) affects the yield of cyclobutyl dimers induced in DNA by 254 nm radiation. The effects are a function of r, the ratio of Cu(II) to DNA phosphate, and of the ultraviolet (UV) fluence; they seem to reflect two types of copper complexes with DNA. The first probably involves "exterior" binding to the bases of native DNA and increases [unk]TT formation (without affecting [unk]UT yield) by raising the energy levels of bases other than thymine. The second seems to occur only at high ratios (rs) and only after the structure has been opened locally by UV radiation; it involves "interior" binding of Cu(II) to the bases. This complex tends to decrease dimer yield by holding the bases apart and/or by lowering the energy levels of bases other than thymine. These results illustrate the potential use of DNA photoproducts and ligands to probe the structure and interactions of DNA in vitro and perhaps also in vivo.


Urinary tract infections in the newborn: diagnosis from mid-stream urine specimens. Mid-stream urines can be obtained for culture in the majority of neonates with a minimum of effort. Urines collected in plastic bags for cultures showed more than 10,000 organisms per ml. in over 50% of the specimens so obtained from healthy neonates. The use of bags or similar collecting devices should be discouraged. The use of skin disinfectants before obtaining the urine did not decrease the number of contaminants. A good mid-stream urine is more important in avoiding contamination than is the cleansing of the external genitals. Suprapubic aspiration or catheterization should be reserved for those infants in whom (a) repeat mid-stream urine cultures yield equivocal results and (b) the clinical situation does not allow time for repetition of a mid-stream urine culture.


Pulmonary diffusing capacity and the capillary bed during Valsalva and Müller maneuvers.


The subject as a source of bias in psychological research.


Electron microscopic studies on the localization of antibodies in rat lymph node cells producing moloney virus.


The ultrasonic dental unit: a guide for the clinical application of ultrasonics in dentistry and in dental hygiene.


Psychosomatic concepts and dentistry: some perspectives.


Comparative pharmacodynamic activity of single and divided doses of benzphetamine hydrochloride.


Complexation between hexylresorcinol and polyvinylpyrrolidone and its microbiological evaluation.


A three-dimensional matrix for the study of microbial radiation and survival in the dry state.


Some structural variables in auditory projective testing.


Self-images without awareness and projective methods of personality assessment.


Shading as an index of anxiety in figure drawings.


Block form stability in removable partial dentures.


An extraoral pin technique.

An extraoral pin technique.


American Board of Prosthodontics.

American Board of Prosthodontics.


Treatment of maxillomandibular constrictions.


Dentists licensed as dental specialists in prosthodontics.


Blood glucose and drug levels in normal fasting subjects following single oral doses of tolazamide and chlorpropamide.


The clinical spectrum of papillary-muscle disease.


Roentgen alchemy. II.

Roentgen alchemy. II.


Antalgic properties of proxazole. Double blind study in visceral algoplastic conditions.


Endocrine control of calcium and magnesium metabolism in ruminants.


Chronic copper toxicity of ruminants.

Chronic copper toxicity of ruminants.


Urinary calculi in ruminants.

Urinary calculi in ruminants.


Mycotoxins in ruminants.

Mycotoxins in ruminants.


Assessment of protein requirements by nitrogen balance.


Enzymes and the assessment of protein nutriton.


The control of haemoglobin synthesis: factors controlling the output of alpha and beta chains.


Complementary ways of meeting the world's protein need.


Object loss, dreaming, and creativity. The poetry of John Keats.


A study of cultures of rumen anaerobic bacteria in the presence of excess riboflavine (vitamin B2).


Visual intensity judgments: an empirical rule and a theory.


Seal stones of the Minoan period in the Ashmolean Museum, Oxford, depicting physical activities.


Intentional communication in the tree drawing test.


Fixation of microscopic fresh-water green algae by 31 per cent OsO4 in CCl4 in an unbuffered, two-phase fixative system.


Rapid alkaline rehydration of dried plant tissues for histologic study.


Peyronie's disease.

Peyronie's disease.


Current practice in tropical medicine.

Current practice in tropical medicine.


Management of chronic pain.

Management of chronic pain.


Scintillography, thermography and ultra sound in diagnosis.


Ultrasonography and thermography in medical diagnosis.


The Medical Society's transactions.

The Medical Society's transactions.


Pulmonary embolization following inferior vena caval interruption.


Fluorescent antibody technic in diagnosis of canine distemper.


A technic for repair of ruptured common bile duct in a dog (case report).


Severe soft tissue avulsion injury to the lower extremity with circumferential skin loss. Report of two cases in children with successful rehabilitation.


L-dopa and amantadine in the therapy of parkinsonism.


Phase microscopic and electron microscopic studies of bacterial phagocytosis by leucocytes. II. Behavior of leucocytes, expecially their granules, following phagocytosis.


Schoosl of public health in the USA and Canada (year ending June 1969).


Planning health care for veterans.

Planning health care for veterans.


A selected public health bibliography with annotations.


Thoracic outlet compression syndrome.

Thoracic outlet compression syndrome.


Renal osteodystrophy; some therapeutic consideration relative to long-term dialysis and transplantation.


Heart disease in patients with seropositive rheumatoid arthritis; a controlled autopsy study and review.


Local chemotherapy omaduromycosis caused by Monosporium apiospermum.


The psychology of terminal illness as portrayed in Solzhenitsyn's The Cancer Ward.


Primary phase of rennin action on whole milk.


Action of rennin on heat-sterilized milk.


Methods for the analysis of deoxyribonucleic acid-ribonucleic acid hybridization data.


Transcription of bacteriophage T4 deoxyribonucleic acid in vitro. 1. RNA was synthesized in vitro from a template of bacteriophage T4 DNA, in the presence of Mn(2+). A comparison was made of the RNA synthesized by purified RNA polymerase from two sources, Micrococcus lysodeikticus and Escherichia coli; these are referred to as Micrococcus cRNA and E. coli cRNA respectively (where cRNA indicates RNA synthesized in vitro by using purified RNA polymerase and a DNA primer). 2. Both types of RNA were self-complementary as judged by resistance to digestion with ribonuclease after self-annealing, Micrococcus cRNA being more self-complementary (40%) than was E. coli cRNA (30%). The cRNA was found to be much less self-complementary if Mg(2+) was present during RNA synthesis instead of Mn(2+). 3. Micrococcus cRNA hybridized with a larger part of bacteriophage T4 DNA than did E. coli cRNA. The E. coli cRNA competed with only part (70%) of the Micrococcus cRNA in hybridization-competition experiments. It is concluded that more sequences of bacteriophage T4 DNA are transcribed by Micrococcus polymerase than by E. coli polymerase. 4. The RNA sequences synthesized by Micrococcus RNA polymerase but not by E. coli RNA polymerase are shown by hybridization competition to compete with specifically late bacteriophage T4 messenger RNA sequences. The relevance of this finding to the control of transcription is discussed. 5. In an Appendix, new methods are described for the analysis of hybridization-saturation and -competition experiments. Particular attention is paid to the effects produced if different RNA sequences are present at different relative concentrations. 6. By using cRNA isolated from an enzymically synthesized DNA-RNA hybrid, it is estimated that, of the DNA that is complementary to cRNA, only about half can become hybridized with cRNA under the experimental conditions used.


An analysis of the ribosomal ribonucleic acids of Escherichia coli by hybridization techniques. From analyses of the hybridization of Escherichia coli rRNA (ribosomal RNA) to homologous denatured DNA, the following conclusions were drawn. (1) When a fixed amount of DNA was hybridized with increasing amounts of RNA, only 0.35+/-0.02% of E. coli DNA was capable of binding (16s+23s) rRNA. Although preparations of 16s and 23s rRNA were virtually free from cross-contamination, the hybridization curves for purified 16s or 23s rRNA were almost identical with that of the parent specimen containing 1 weight unit of 16s rRNA mixed with 2 weight units of 23s rRNA. The 16s and 23s rRNA also competed effectively for the same specific DNA sites. It appears that these RNA species each possess all hybridizing species typical of the parent (16s+23s) rRNA specimen, though probably in different relative amounts. (2) By using hybridization-efficiency analysis of DNA-RNA hybridization curves (Avery & Midgley, 1969) it was found that (a) 0.45% of the DNA would hybridize total rRNA and (b) when so little RNA was added to unit weight of DNA that the DNA sites were not saturated, only 70-75% of the input RNA would form hybrids. The reasons for the discrepancy between the results obtained by the two alternative analytical approaches were discussed. (3) For either 16s or 23s rRNA, hybridization analysis indicated that two principal weight fractions of rRNA may exist, hybridizing to two distinct groups of DNA sites. However, these groups seem to be incompletely divided between the 16s and 23s fractions. Analysis suggested that (a) 85% of the 16s rRNA was hybridized to about half the DNA that specifically binds rRNA (0.23% of the total DNA). (b) 70% of the 23s rRNA hybridized to a further 0.23% of the DNA and (c) the minor fraction (15%) of 16s rRNA may be competitive with the major fraction (70%) of 23s rRNA. Conversely, the minor fraction (30%) of the 23s rRNA may compete with the major fraction (85%) of 16s rRNA. Models were proposed to explain the apparent lack of segregation of distinct RNA species in the two subfractions of rRNA. (4) If protein synthesis and ribosome maturation were inhibited in cells of an RC(rel) mutant, E. coli W 1665, by depriving them of an amino acid (methionine) essential for growth, the inhibition had no discernible effect on the relative rates of synthesis of rRNA species. The rRNA that accumulates in RC(rel) strains of E. coli after amino acid deprivation is apparently identical in its content of RNA species with that of the pre-existing mature RNA in the ribosomes. On the other hand, the messenger RNA is stabilized, and accumulates as about 15% of the RNA formed after withdrawal of the amino acid.


I. The teaching of community medicine in the undergraduate curriculum.


Intestinal suturing. I. The development of intestinal sutures.


Prochymosin and chymosin (prorennin and rennin).


Initiation of hemoglobin synthesis with rabbit and E. coli tRNA in a reticulocyte cell-free system.


The template activity of rat liver chromatin.


Coiling of the flagellar shaft in the head region of the mature sperm of Nucella (purpura) lampillus (L.).


The classes of endosymbiont of Paramecium aurelia.


Chromosome replication, protein synthesis and cell division in Escherichia coli.


Metabolism of hydrocortisone 21-sulfate in man.


Electron microscopic study on gliosome formation in postnatal development of spinal cord in the cat.


Localization of primary sensory afferents in the prothoracic ganglion of the wood ant (Formica lugubris Zett.): a combined light and electron microscopic study of secondary degeneration.


Experimental studies in tumor imminology.


Historical analysis of religion and sex.


Free transplantation of oral mucosa for imporvement of denture retention.


Biological significance of induced human chromosome aberrations.


Protracted survival after homotransplantation of the lung and simultaneous contralateral pulmonary artery ligation.


Correlations between the vitamin D-induced calcium binding protein and intestinal absorption of calcium.


Volume-limited intermittent cuff inflation for long-term respiratry assistance.


Synchronously dividing mammalian cells.

Synchronously dividing mammalian cells.


The autoperfusing heart-lung preparation: a vehicle for the preservation of the resuscitated cadaver heart.


Survey of some spontaneous and experimental disease processes of lower vertebrates and invertebrates.


History of ophthalmology in St. Louis. II.


Acute leukemia, 1969. A review.

Acute leukemia, 1969. A review.


General problems of transplantation immunity.


Clinical problems of transplantation.

Clinical problems of transplantation.


Transcription of the bacteriophage T4 template: strand selection by E. coli RNA polymerase in vitro.


Kidney allograft survival in the dog predicted by mixed leukocyte culture tests.


Physiological and genetic implications of selective mutation by light at 320-400 nm.


Microorganisms, alive and imprisoned in a polymer cage.


Castration, sex hormones, and tubero-infundibular dopamine neurons.


Internal feedback of prolactin in the rat.


Nutritional status--Japan.

Nutritional status--Japan.


Lactase deficiency in Thailand.

Lactase deficiency in Thailand.


Sodium intake and blood pressure.

Sodium intake and blood pressure.


Environment and growth.

Environment and growth.


Body composition and high carbohydrate diets at high altitude.


Riboflavin--transport and excretion.

Riboflavin--transport and excretion.


Response of human beings to vitamin K 1.


Phospholipid synthesis in microsomes.

Phospholipid synthesis in microsomes.


Calcium-fluoride interactions in kittens.


Measurement of phenylalanine hydroxylase activity in mammalian cells.


Medical management of cardiac transplant patients.


Advances in obstetrics and cynaecology.

Advances in obstetrics and cynaecology.


Advances in surgery.

Advances in surgery.


Advances in paediatrics.

Advances in paediatrics.


Advances in the treatment of cardiovascular disease.


Advances in the treatment of peripheral vascular disease.


Advances in antibiotics.

Advances in antibiotics.


Advances in general practice.

Advances in general practice.


Advances in child psychiatry.

Advances in child psychiatry.


Advances in geriatrics.

Advances in geriatrics.


Advances in industrial medicine.

Advances in industrial medicine.


Advances in neurosurgery.

Advances in neurosurgery.


The action of methysergide in manic states.


Clinical response to long-term propranolol therapy in hyperthyroidism.


A clinical trial of methysergide and lithium in mania.


Human gastric intrinsic factor secretion.


A comparison between the effects of secretin and histamine on the gastric secretion of pepsin in man.


Was Machiavelli right?

Was Machiavelli right?


A proven new approach to removable fixed bridge construction using pre-fabricated parts.


The assessment of social disability.

The assessment of social disability.


Catecholamine metabolism in affective disorders. II. Norepinephrine, normetanephrine, epinephrine, metanephrine, and VMA excretion in hypomanic patients.


Antiulcerogenic and pepsin inhibitor properties of lignosulfonates.


Endonucleolytic cleavage of UV-irradiated DNA controlled by the V+ gene in phage T4.


Interrelationship between magnesium and polyamines in a pseudomonad lacking spermidine.


An isotope dilution procedure for detection and quantitative estimation of phenylthiohydantoins released in the Edman degradation.


Restriction of bacteriophage phiX174 by F factor.


The measurement of cardiac output.

The measurement of cardiac output.


Regional blood flow measurement in clinical practice.


Current concepts in the primary treatment of operable carcinoma.


Special problems in full rehabilitation of the nasal base and upper lip in single and double clefts.


Splinting--a review and clinical investigation--preliminary report.


The association's gavel. A description and its history.


François Mauriceau (1637-1709) Parisian accoucheur.


The survival time of platelets in experimental mural thrombi.


Detection of circulatory shunts by means of external counting method. Differentiation between ASD and VSD, semiquantitative estimation of left-to-right shunt, and the detection of right-to-left shunt.


Famous men and their poor health.

Famous men and their poor health.


Childhood rickets: revival of an ancient menace.


Regional cerebral blood flow measurement with small probes. I. Evaluation of the method.


Clinical evaluation of albutoin.

Clinical evaluation of albutoin.


Systems thinking. New dimension in hospital functions.


Flurandrenolone tape in treatment of psoriasis.


Introduction of the Rudolf Virchow lecturer. President's address.


Antibiotics today.

Antibiotics today.


In memoriam--Kurt Goldstein.

In memoriam--Kurt Goldstein.


In memoriam Paul Hoch.

In memoriam Paul Hoch.


Kurt Goldstein.

Kurt Goldstein.


Professor Herbert Elias on his 80th birthday.


Ethylestrenol (Orgabolin): effects on asthmatic children during corticosteroid treatment.


The structure of a photoreceptor organelle in the eye of Pterotrachea mutica.


Ontogeny of the mouse motor cortex. The polymorph layer or layer VI. A Golgi and electronmicroscopical study.


Healing of skin incisions during reduced tension of the wound area. A tensiometric and histologic study in the rat.


Enzymatic release of phosphorus from human dental enamel by enzyme preparations of E. coli. A preliminary report.


Dimethyl sulfoxide (DMSO) ointment in the treatment of rheumatoid arthritis. A double blind study.


High incidence of chromophobe pituitary adenoma-like lesions in an inbred Sprague-Dawley breeding rat colony.


Susceptibility of mink to Clostridium botulinum type E toxin.


Dihydrodipicolinic acid synthase of Bacillus licheniformis.


Experiences in the treatment of alcoholics with methylperidol (Luvatren).


Observations on the fine structure of rat bulbourethral gland cells.


N-lipoyl glucosamine and N-lipoyl glucosamitol: substrates for lipoyl dehydrogenase.


Inhibition by aminochromes of in vitro polypeptide synthesis in Escherichia coli.


Turnover of UDP-sugars in E.

Turnover of UDP-sugars in E.


Edward Lawrie of the Hyderabad Chloroform Commission.


Pre-operative respiratory function tests and postoperative mortality. A study of patients undergoing surgery for carcinoma of the bronchus.


The effects of intermittent positive pressure ventilation on lung function in hyaline membrane disease.


Studies of drugs given before anaesthesia. 18. The synthetic opiates.


Active immunotherapy in treatment of acute leukaemia. Active non-specific immunotherapy has been used to prolong remissions in acute lymphoblastic leukaemia. The series reported here used Bordetella pertussis vaccine in a controlle trial after intensive chemotherapy. Possibly immunotherapy delayed the onset of relapse in the treated patients, but no long-term remissions were obtained. Further work is needed to establish the role of immunotherapy in general, and the use of B. pertussis vaccine in particular, in the treatment of acute leukaemia.


Renal transplantation and leucocyte migration.


Saving the grossly disabled.

Saving the grossly disabled.


Spontaneous decrease in gastric secretory response to humoral stimuli. The gastric response to pentagastrin was studied over a period of seven months in a healthy 25-year-old man without symptoms or history of gastrointestinal disease. An abrupt impairment of the gastric response to several stimulants was observed one month after starting the tests. Periodic testing since that time has shown no reversion to the type of response seen during the initial period of testing. A second subject with proved chronic duodenal ulceration presented an identical change in pattern of gastric response to stimulants.This study suggests that such a variation in the response to gastric stimulation in a subject with a normal stomach should be borne in mind when interpreting results of gastric secretory studies.


Quick- and slow-release iron: a double-blind trial with a single daily dose regimen. In a double-blind trial the response to treatment with Ferro-Gradume (a slow-release preparation of ferrous sulphate) has been compared in the same individuals with the response to ferrous fumarate in a single daily dose regimen using a similar dose of iron. No significant difference was found in the therapeutic response or in the incidence of side-effects between the two preparations.


Single-blind comparative trial of trimethoprim-sulphamethoxazole and ampicillin in the treatment of exacerbations of chronic bronchitis. Fifty patients with exacerbations of chronic bronchitis were treated with either a combination of trimethoprim 320 mg. and sulphamethoxazole 1,600 mg. a day or ampicillin 2 g. a day. The trial, carried out as a single-blind procedure, showed that the combination was more effective as judged by clinical response and reduction in sputum volume and purulence, with eradication of pathogenic organisms. No appreciable side-effects were encountered with either treatment, and it is suggested that the trimethoprim-sulphamethoxazole combination may be a safe and useful drug in the treatment of chronic bronchitis.


Use of antilymphocyte globulin after cadaveric renal transplantation. Antilymphocyte globulin (A.L.G.) was prepared by injecting fresh frozen splenic cells subcutaneously into horses. The IgG fraction of the serum was concentrated by a batch technique using diethylaminoethanol-Sephadex. Fourteen patients given this material by intramuscular injection after cadaveric renal transplants, in addition to azathioprine and prednisone, had less evidence of rejection compared with patients previously treated with azathioprine and prednisone only, despite a reduction of the mean daily prednisone dose from 65 to 45 mg. Toxicity, especially local reaction, fever, and hypotension, limited the amount of A.L.G. that was given.


History of venous research.

History of venous research.


Subcellular localization of proinsulin to insulin conversion in isolated rat islets.


Upper respiratory tract infections.

Upper respiratory tract infections.


Regulatory role of adenine nucleotides in the biosynthesis of guanosine 5'-monophosphate. Derepression of the synthesis of inosine 5'-monophosphate (IMP) dehydrogenase and of xanthosine 5'-monophosphate (XMP) aminase in pur mutants of Escherichia coli which are blocked in the biosynthesis of adenine nucleotides and guanine nucleotides differs in two ways from derepression in pur mutants blocked exclusively in the biosynthesis of guanine nucleotides. (i) The maximal derepression is lower, and (ii) a sharp decrease in the specific activities of AMP dehydrogenase and XMP aminase occurs, following maximal derepression. From the in vivo and in vitro experiments described, it is shown that the lack of adenine nucleotides in derepressed pur mutants blocked in the biosynthesis of adenine and guanine nucleotides is responsible for these two phenomena. The adenine nucleotides are shown to play an important regulatory role in the biosynthesis of guanosine 5'-monophosphate (GMP). (i) They induce the syntheses of IMP dehydrogenase and XMP aminase. (The mechanism of induction may involve the expression of the gua operon.) (ii) They appear to have an activating function in IMP dehydrogenase and XMP aminase activity. The physiological importance of these regulatory characteristics of adenine nucleotides in the biosynthesis of GMP is discussed.


Evidence for two genes specifically involved in unsaturated fatty acid biosynthesis in Escherichia coli. Unsaturated fatty acid auxotrophs of Eschericha coli have been divided into two distinct cistrons by extract complementation and genetic complementation based on abortive transduction. Lesions in one cistron result in the loss of the beta-hydroxydecanoyl thioester dehydrase which produces the first biosynthetic intermediate in unsaturated fatty acid formation. Evidence is presented which indicates that lesions in the second cistron result in the lack of a second enzyme specifically involved in the biosynthesis of unsaturated fatty acids.


Polarity in gene araB of the l-Arabinose operon in Escherichia coli B/r. A series of mutations are described which map in the araB gene of the l-arabinose operon and exert a polar effect on gene araA, the structural gene for the l-arabinose isomerase. Ten of the 20 araB point mutants examined exhibited absolute polarity and may represent insertions of genetic material into the araB gene. The remaining 10 point mutants exhibit strong polarity (less than 10% of the normal wild-type inducible level of isomerase) and may represent a class of externally suppressible polar mutations other than amber or ochre. Seven of the 12 araB deletion mutants examined, or 58%, exhibit polarity, suggesting that a shift in the reading frame has been generated in the polycistronic message for the l-arabinose operon. The remaining, presumably in-phase, deletion mutants exhibit hyperinducible levels of isomerase, an effect that is eliminated when an araB(+) gene is introduced in the trans position. The hyperinducibility effect is discussed in terms of a model for self-catabolite repression, originally proposed by Katz and Englesberg.


Deoxycholate-treated, nontoxic, whole-cell vaccine protective against experimental salmonellosis of mice. A vaccine prepared from the residue after extraction of whole cells of Salmonella typhimurium with 2% sodium deoxycholate proved to be nontoxic and highly immunogenic. The material was not lethal for mice at 6.0 mg and was essentially nontoxic in rabbit skin, whereas endotoxic activity was found in the dialyzed extract. A high dosage, above 2.0 mg, was less protective than lower doses, indicating a degree of "immunologic paralysis." Three inoculations of low doses, 0.25 mg each, induced protection against death and tissue infection in animals challenged with 2,000 ld(50) of virulent homologous S. typhimurium and against death, but not against tissue infection, after heterologous challenge with S. enteritidis. Residues of purified cell walls were as effective as residues of whole cells, indicating that the immunizing antigen(s) resided in the cell wall.


Genetic and molecular properties of an infectious antibiotic resistance (R) factor isolated from Klebsiella. A Klebsiella strain of human origin that was resistant to ampicillin, chloramphenicol, kanamycin, neomycin, streptomycin, and tetracycline was found to have all of these resistances associated with a R factor and a satellite molecular species of deoxyribonucleic acid (DNA) with an average buoyant density of 1.710 in cesium chloride gradients. There was no evidence of the existence of DNA with other buoyant densities. The strain bears two separable mutations for chloramphenicol resistance, both of which are associated with the R factor (KR9). Exposure of the Klebsiella strain to acridine derivatives or to ethidium bromide (which was more efficient) resulted in partial losses of resistance accompanied by the disappearance of the satellite DNA peak or shifts in its density. The R factor and its component genes were conjugally transmitted across generic boundaries and maintained in new hosts with different efficiencies. The basis of this difference lies not only in the efficiency of conjugal transfer but also in the stability of the components after transfer. All of the resistance genes and the resistance transfer factor were cotransducible by phage Plkc from Escherichia coli. Partially resistant strains could be reconstituted to full resistance or to a recombined pattern of partial resistance by conjugation with donors having complementary resistance patterns. This recombination serves as an efficient mechanism for rescuing superinfecting genes that are otherwise intracellularly excluded. KR9 is an fi(+) type of R factor which in the natural state does not appear to be as repressed in conjugal transfer as other R factors.


Mouse virulence of K(L) antigen-containing strains of Escherichia coli. The mouse virulence of two K antigen-containing (L variety) strains of Escherichia coli (serotype O2:K1) isolated from human septicemia, and of their variants which lacked K antigen, was studied. The strains containing envelope antigen (K+) were highly virulent when injected intracerebrally or when suspended in mucin and injected intraperitoneally. After intraperitoneal injection of E-107 K+ (but not K-), there was a marked initial growth in the peritoneal cavity followed by bacteremia and infection of all the organs examined. In the mucin-enhanced lethal infection, this growth continued until death of the animal; in the nonlethal infection, growth ceased and the count dropped quickly after approximately 5 hr. Host defenses were depressed greatly by intraperitoneally, but not intravenously, administered mucin. Bacteria were most virulent when injected intraperitoneally. In vitro phagocytosis of the K+ bacteria required opsonins not needed for phagocytosis of the smooth K- variants. Opsonins were found in immunized rabbit and normal mouse sera. Immune rabbit sera contained antibodies with anti-K specificity which were opsonic in vitro and highly protective in vivo when administered passively. There appears to be a lesser anti-O opsonic and protective activity involving one of the strains (E-107 K+), and colonial morphology, agglutination, and absorption tests indicated a low amount of K antigen on this organism. No anti-O opsonic or protective activity could be shown involving the other strain (E-102 K+). When standard serological typing procedures were used, these two strains appeared to be identical serologically, but they differed greatly in sensitivity to immune rabbit serum in phagocytosis experiments in vitro.


Isolation and characterization of host-independent Bdellovibrios. A reliable method has been developed for the isolation of host-independent (H-I; i.e., "saprophytic") strains of Bdellovibrio from host-dependent (H-D; i.e., "parasitic") cultures. The technique involves growing streptomycin-resistant (Sm(r)) H-D cultures on streptomycin-susceptible (Sm(8)) host cells. A lysate containing large numbers of the Sm(r) H-D cells and some remaining Sm(8) host cells is transferred to a selection medium which contains the antibiotic. The Sm(8) host cells in the lysate are killed, and the Sm(r) H-I strains develop in broth within 3 to 6 days. By use of this method, it has been possible to isolate H-I strains from 16 different H-D Bdellovibrio strains studied. The frequency of occurrence of host independence is in the range of one H-I colony per 10(6) to 10(7) plaque-forming units of H-D bdellovibrios. The H-I cultures are nonfermentative, do not reduce nitrate, are strongly proteolytic, are oxidase-positive, and do not utilize 14 different carbon compounds as sources of energy for growth. Most H-I cultures are catalase-positive upon initial isolation from H-D lysates, but some cultures lose this enzyme upon subsequent transfers through host-free media. Most H-I bdellovibrios are pleomorphic, consisting of vibrio- to spiral-shaped cells typically measuring 0.3 to 0.4 mum in width and 1 to 10 mum in length. All H-I bdellovibrios have a cytochrome a and c component (H-I A3.12 differs from the other strains in the location of the peaks of the cytochrome spectrum). All are sensitive to oxytetracycline and (except for strain H-I A3.12) to the vibriostatic pteridine 0/129; most bdellovibrios, except for H-I A3.12, are generally uniformly resistant or susceptible to a given antibiotic. Bdellovibrio and Vibrio spp. have common cytochrome difference spectra and susceptibilities to oxytetracycline and to the vibriostatic pteridine 0/129. All H-I bdellovibrios examined produce an exocellular protease which digests heat-killed host cells. Bdellovibrios possessing predatory and bacteriolytic properties could be reselected from H-I bdellovibrio cultures growing in the presence of living host cells. Attempts to select for bacteriolytic isolates from Vibrio and Spirillum spp. were unsuccessul.


Deoxyribonucleic acid characterization of Bdellovibrios. The guanine plus cytosine (GC) content of the deoxyribonucleic acid (DNA) of 11 isolates of host-dependent (H-D) bdellovibrios and 18 host-independent (H-I) derivatives was determined from thermal denaturation curves and buoyant densities in CsCl. The H-D and respective H-I cultures have GC contents which are identical within the limits of experimental error. Most cultures of Bdellovibrio bacteriovorus, including the holotype culture, have 50.4 +/- 0.9 moles% GC in their DNA; two bdellovibrio isolates of presently uncertain nomenclatural status contain DNA of about 43% GC. Optical melting profiles of all the DNA from all of these organisms are particularly steep, indicating little compositional heterogeneity. Chromatography of acid hydrolysates of Bdellovibrio nucleic acids reveal no unusual components. The DNA content per cell of one H-I derivative is about one-third the amount per Escherichia coli cell growing at a comparable rate.


Circular deoxyribonucleic acid from Shigella dysenteriae Y6R. Circular deoxyribonucleic acid was isolated from Shigella dysenteriae Y6R and was found to consist of six species having molecular weights of 10(6), 1.3 x 10(6), 2.6 x 10(6), 3.8 x 10(6), 20 x 10(6), and 24 x 10(6) daltons. These size classes were partially resolved by sucrose density gradient centrifugation. The minicircles (10(6) and 1.3 x 10(6)) were found to have a buoyant density in CsCl of 1.710 g/ml. The 3.8 x 10(6) dalton class had a density of 1.707 g/ml. The two largest species had a density of 1.702 g/ml. Two other strains, S. sonnei II and S. dysenteriae 60, also contained circular deoxyribonucleic acid.


Catabolite repression gene of Escherichia coli. A catabolite repression gene (cat) which alters the sensitivity of Escherichia coli to catabolite repression has been mapped by transduction and shown to be located between the pyrC and purB genes. When the cat-1 mutation was studied in a number of genetic backgrounds, the results showed that this mutation affects the synthesis of more than one catabolic enzyme but does not completely eliminate catabolic repression under all conditions. It is suggested that this mutation may cause a block in the accumulation of the catabolite effector. Our experiments show that this effector is not glucose-6-phosphate.


Bicomponent nature of lysin from Streptococcus zymogenes. Nonlytic mutants of Streptococcus zymogenes X-14 were isolated after exposure to nitrosoguanidine. Cross-streaking of certain of these mutants on brain Heart Infusion (BHI) blood agar plates resulted in formation of spur-shaped zones of hemolysis at the junction of the two streaks. Two types of mutants were recognized. Both of these excreted into the medium substances which are nonlytic but which together produce lytic activity. These substances behaved as an enzyme with an activator. Hence, one mutant type appeared to produce an activator and the other the catalytic molecule. Active complexes were temperature-sensitive and were inhibited by some teichoic acids as is the parental type of lysin.


Specific action of sodium dodecyl sulfate on the  sex factor of Escherichia coli K-12 Hfr strains. A specific action of sodium dodecyl sulfate (SDS) on the sex (F) factor in the integrated state of Escherichia coli K-12 Hfr H strain is reported. Growth of Hfr cells in Penassay Broth containing SDS results in the elimination of part or all of the F factor, yielding low and nonfertile variants of defective Hfr type and F(+) cells and also F(-) derivatives. Appearance of such variants was generally observed after the culture reached stationary phase. The frequencies of F(-) cells then increased. F(-) cells were usually isolated as the major population among survivors. Some defective variants of Hfr cells with an intermediate fertility between standard Hfr and F(+) cells had lost sensitivity toward the male-specific ribonucleic acid phage M12. Other defective Hfr variants with as much or less fertility than standard F(+) cells had also all lost sensitivity to phage M12. On single-colony isolation, they segregated nonfertile female H cells which, when infected with F, could restore high fertility with oriented transfer of the chromosome the same as that of the original Hfr H. Also, sensitivity to phage M12 was regained. Female H cells were characterized as those lacking fertility but still retaining a small segment of F or sfa locus at the original part of the chromosome, where newly infected F could attach. Similar results were obtained with two other Hfr strains. A possible mechanism of the specific action of SDS is discussed.


Genetic analysis of recombination-deficient mutants of Escherichia coli K-12 carrying rec mutations cotransducible with thyA. The rec mutations carried by 20 strains of Escherichia coli K-12 which are defective in genetic recombination and sensitive to ultraviolet light and X rays, and whose lambda lysogens show spontaneous phage production, have been mapped near thyA. In 15 of the strains, the rec mutation fails to complement recB21 but complements rec-22. The other five strains carry a rec mutation which complements recB21 but not rec-22. These mutations map closer to thyA than those which fail to complement recB21. They therefore appear to be defective in a different recombination gene, denoted recC. The order of recB and recC on the linkage map of E. coli K-12 is thyA-recC-recB-argA.


Catabolite inhibition: a general phenomenon in the control of carbohydrate utilization. When Escherichia coli is grown in synthetic medium with radioactive galactose or lactose as the carbon source, the addition of glucose rapidly inhibited utilization of the radioactive substrate, whether the formation of (14)CO(2) or acid-insoluble products was measured. The inhibition was reversed after the removal of glucose. Experiments with mutants blocked in subsequent steps of galactose and lactose metabolism demonstrated that the inhibition occurs prior to the formation of the first metabolic product. The utilization of a variety of sugars, including maltose, lactose, mannose, galactose, l-arabinose, xylose, and glycerol was inhibited by glucose. Of a number of carbohydrates tested as potential inhibitors, only glucose and, to a lesser extent, glucose-6-phosphate (G-6-P) were capable of inhibiting the utilization of all of the substrates. Glucose did not inhibit G-6-P utilization but G-6-P inhibited glucose utilization. With all substrates, except glycerol, there was a delay before the onset of inhibition by G-6-P. We conclude that E. coli has a general regulatory mechanism, termed catabolite inhibition, which controls the activity of early reactions in carbohydrate metabolism, allowing certain substrates to be utilized preferentially.


Inhibitory effect of penicillin on proline synthesis in Escherichia coli. The production of glutamic gamma-semialdehyde, an intermediate in the synthesis of proline, was inhibited in Escherichia coli by physiological concentrations of penicillin. Sucrose (0.6 m) and sodium chloride (0.1 m) prevented penicillin inhibition of glutamic gamma-semialdehyde synthesis. Cells which were in the stationary phase, or which had been permitted to metabolize without growth, were insensitive to the effects of penicillin on glutamic gamma-semialdehyde synthesis.


Inhibitor-induced shift-downs in Escherichia coli. A shift-down response in Escherichia coli cells has been brought about by moderate concentrations of azide or cyanide. Early events of the response included a preferential inhibition of ribonucleic acid relative to deoxyribonucleic acid synthesis, a degradation of polyribosomes, and an inhibition of protein synthesis followed by a transient relief. These changes were entirely comparable to those observed with nutrient-induced shift-downs. The influences of various nutrient supplements on an azide shift-down were examined, and methionine was found to relieve effectively the inhibition of ribonucleic acid synthesis in some strains of cells.


Technique for the isolation of ribonucleic acid-rich mutants from Escherichia coli. A technique for the isolation of ribonucleic acid (RNA)-rich mutants of Escherichia coli is described. Mutagenized cells were centrifuged to isopycnic equilibrium on potassium tartrate or cesium sulfate gradients. Samples from the region of the gradients slightly denser than the majority of the cells were spread on agar plates, and the resulting clones were tested for increased RNA to protein ratios.


Partial separation of two pools of arginine in Escherichia coli; preferential use of exogenous rather than endogenous arginine for the biosynthesis of 1,4-diaminobutane.


Acyl carrier protein. 13. Beta-ketoacyl acyl carrier protein synthetase from Escherichia coli.


Purification and properties of anthranilate synthase from Salmonella typhimurium.


Origin, development, and nature of intranuclear rodlets and associated bodies in chicken sympathetic neurons. Correlative data are presented here on the developmental history, dynamics, histochemistry, and fine structure of intranuclear rodlets in chicken sympathetic neurons from in vivo material and long-term organized tissue cultures. The rodlets consist of bundles of approximately 70 +/- 10 A proteinaceous filaments closely associated with approximately 0.4-0.8 micro spheroidal, granulofibrillar (gf) bodies of a related nature. These bodies are already present in the developing embryo a week or more in advance of the rodlets. In early formative stages rodlets consist of small clusters of aligned filaments contiguous with the gf-bodies. As neuronal differentiation progresses these filaments increase in number and become organized into well-ordered polyhedral arrays. Time-lapse cinemicrography reveals transient changes in rodlet contour associated with intrinsic factors, changes in form and position of the nucleolus with respect to the rodlet, and activity of the gf-bodies. With the electron microscope filaments may be seen extending between the nucleolus, gf-bodies, and rodlets; nucleoli display circumscribed regions with fine structural features and staining reactions reminiscent of those of gf-bodies, We suggest that the latter may be derivatives of the nucleolus and that the two may act together in the assemblage and functional dynamics of the rodlet. The egress of rodlet filaments into the cytoplasm raises the possibility that these might represent a source of the cell's filamentous constituents.


A study of the T system in rat heart.

A study of the T system in rat heart. The technique of extracellular space tracing with horseradish peroxidase is adapted for labeling the transverse tubular system (T system) in rat heart. In rat ventricular muscle the T system shows extensive branching and remarkable tortuosity. The T system can only be defined operationally, since it does not display specific morphological features throughout its entire structure. Owing to branching of the T system, a sizable proportion of the apposition between the T system and L system (or closed system) occurs at the level of longitudinal branches of the T system and is not restricted to the Z line region. The regions of apposition between the T system and L system are analyzed in rat ventricular muscle and skeletal muscle (diaphragm) and compared with the intercellular tight junctions (nexuses) of heart muscle by the use of a photometric method. The over-all thickness of the nexus is significantly smaller than that of T-L junctions in both cardiac and skeletal muscles. The thickness of the membranes of the T and L systems are not significantly different in the two muscles, but the gap between both membranes is larger in the heart. In atrial muscle the following two types of cells are found: (a) those cells with a well-developed T system in which the tubular diameter is quite uniform and the orientation predominantly longitudinal and, (b) cells with no T system, but with a well-developed L system. Atrial cells possessing a T system are richly provided with specific granules and show little micropinocytotic activity, whereas cells devoid of T system show intense micropinocytotic activity and few specific granules. The possible functional implications of these findings are discussed.


Enzymatic digestion of desmosome and hemidesmosome plaques performed on ultrathin sections.


A brief note on the development of neurosurgery in Soviet Union.


Locus and state of aggregation of myosin in tissue sections of vertebrate smooth muscle. Structures with the characteristics of molecular myosin were identified by electron microscopy in tissue sections of vertebrate smooth muscle. No thick filaments of myosin were found regardless of preparative procedures, which included fixation at rest and in contraction, glycerine extraction, and storage at low pH prior to fixation. Absence of thick myosin filaments and presence of what appear to be myosin molecules is in accord with conclusions based on X-ray diffraction (3, 12) and birefringence data (4) from living smooth muscles at rest and in contraction. Explanations are provided for appearances thought by others (6, 20, 21) to represent thick myosin filaments. Our present observations are in accord with the model for smooth muscle contraction which we have previously proposed (1).


Artificial arms--are they practical?

Artificial arms--are they practical?


Walter Hood Fitch (1817-1892), botanical artist.


Similarities between neurologic effects of L-dopa and of apomorphine.


Contamination of commercially packaged urinary catheter kits with the pseudomonad EO-1.


Style of references standardized.

Style of references standardized.


Validity of the Draw-a-Person test as a measure of body image.


Size of human figures drawn before and after stress.


Frank Billings, a leader in Chicago medicine.


James A. Rooney. 1916-1969.

James A. Rooney. 1916-1969.


Selective transport of gamma A.

Selective transport of gamma A.


Antigenicity of canine tissue cultures.

Antigenicity of canine tissue cultures.


T antigen from nuclear and cytoplasmic extracts from an adenovirus type 12 transformed cell line.


Distribution of blood group A antigen in the epithelial mucins of the digestive tract of Sprague-Dawley rats.


The interaction of platelets, Salmonella, and mouse peritoneal macrophages.


The evolution of hermaphroditism among animals.


Washout of anatomical dead space for alveolar hypoventilation: preliminary case report.


Kasugamycin resistance: 30S ribosomal mutation with an unusual location on the Escherichia coli chromosome. A mutation in Escherichia coli to resistance to the aminoglycoside antibiotic kasugamycin alters the 30S ribosomal subunit. Though all other known 30S ribosomal mutations are located in a cluster in the streptomycin region, kasugamycin resistance is located at a distance from this region, near the leucine region.


Ligand-induced maturation of threonine deaminase. The dimeric intermediate substructures of threonine deaminase, which are obtained by alkaline dialysis of the native tetrameric enzyme, are inactive after reassembly unless they are subsequently exposed to maturation-inducing ligands for which the enzyme possesses stereospecific binding sites. The binding of the ligands at these sites precedes the full maturation of the enzyme.


Mycoplasmosis: cervical and uterine inoculation of heifers with a Danish strain of Mycoplasma bovigenitalium.


Degenerative changes in nucleus medialis dorsalis after bilateral lesions of the orbito-frontal cortex of the cat.


Pituitary-adrenal function in acute falciparum malaria.


Dichlorvos as a single-dose intestinal anthelmintic therapy for man.


Housing and certain socioenvironmental factors and prevalence of enteropathogenic bacteria among infants with diarrheal disease in Panama.


Enteropathogenic bacteria associated with diarrhea among infants in Panama.


A new test of rejection for implants in the anterior chamber of the eye: application for the study of immunological mechanisms operative in a privileged site.


Blood flow measurements in canine pancreatic allografts.


Subacute multifocal leukoencephalopathy with widespread intranuclear inclusions.


Bell's palsy and Bell's phenomenon.

Bell's palsy and Bell's phenomenon.


Chronic progressive external ophthalmoplegia. A critical review.


Three-colour fluorescent labelling method for calcified tissues in a reptile, Caiman crocodilus.


The orbit.

The orbit.


An experiment with an advance letter of an interview inquiry.


Preliminary impressions for complete dentures.


American presidential assassinations.

American presidential assassinations.


Aetiological aspects of diarrhoea in infants and children under 5 years.


Sensitivity of E. coli and salmonellae isolated from camel (Camelus dromedarius) to various antibiotics.


Robert Newton, M.D., Ch.B., F.R.C.O.G.

Robert Newton, M.D., Ch.B., F.R.C.O.G.


Advancement of the upper vagina in the treatment of haematocolpos and haematometra caused by vaginal aplasia. Pregnancy following the construction of an artificial vagina.


On the submicroscopic structure of the cuticular cell wall of Haloxylon salicornicum and Hyoscyamus muticus.


The fine structure of epidermal cell wall in some leguminous seed coats.


The coronary arteriogram (second of two parts). Structural and functional aspects.


Salbutamol and isoproterenol. A double-blind trial to compare bronchodilator and cardiovascular activity.


Physiologic consequences of renal arterial stenosis.


Intermittent positive-pressure respiration and ADH.


Mild thalassemia--a guide to therapy.

Mild thalassemia--a guide to therapy.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 52-1969.


Renal tubular acidosis. Mechanisms, classification and implications.


Coagulation in renal-allograft rejection.


More on mace.

More on mace.


Chemical Mace.

Chemical Mace.


The effect of social isolation on resistance to some infectious diseases.


Importance of the bursa of fabricius in resistance to disease. 2. Resistance to two bacterial diseases.


Furaltadone and tylosin tartrate in the control of experimentally induced chronic respiratory disease complex in chickens.


Transplantation of an unpaired organ, the heart.


Transplantation of a paired organ, the kidney.


Ethical aspects and sociological implications of organ transplantation as a therapeutic procedure.


Fluorometric quantitation of fluorescein-coupled antibodies attached to the cell membrane. A method was devised which allows the measurement, by direct and indirect fluorescence assay, of the binding of fluorescein-coupled antibodies directed against cell-membrane antigens. The systems used were: (1) species-specific antigens, (2) genetically determined isoantigens, (3) virus-determined, tumor-specific antigens, and (4) membrane-bound immunoglobulin in a Burkitt cell line. Titration of antibodies gave straight lines in the log versus log scale, the slope of the titration curve probably being dependent on the density of antigen receptors on the cell surface.


Acetyl CoA carboxylase, II. Deomonstration of biotin-protein and biotin carboxylase subunits. Previous work has shown that Escherichia coli acetyl CoA carboxylase is composed of two dissimilar protein components, E(a) which contains covalently bound biotin and forms E(a)-CO(2)-from HCO(3)- and ATP, and E(b) which is involved in the transfer of the carboxyl group from E(a)-CO(2)- to acetyl CoA, forming malonyl CoA. E(a) has been dissociated into two subunits at pH 9. One subunit, designated biotin carboxylase, catalyzes a model reaction, the ATP-dependent carboxylation of free (+)-biotin. The other subunit contains covalently bound which is carboxylated by the biotin carboxylase in the course of acetyl CoA carboxylation.


Bacteriophage T4 DNA-dependent in vitro synthesis of lysozyme. A cell-free system derived from uninfected Escherichia coli previously was shown to synthesize beta-glucosyl transferase in response to T4 DNA. This same in vitro system, when incubated at slightly higher magnesium concentrations, also synthesized enzymatically active lysozyme. The lysozyme activity that appeared was judged to be T4-specific since antibodies prepared against authentic T4-lysozyme inactivated the in vitro synthesized enzyme. DNA from a T4 mutant carrying a deletion in the lysozyme gene stimulated amino acid incorporation to the same extent as wild-type T4 DNA but was inactive in directing the synthesis of lysozyme. Various inhibitors of RNA and protein synthesis inhibited the in vitro synthesis of lysozyme.


Transfer RNA: A comparison by gel electrophoresis of the tRNA in HeLa cytoplasm, HeLa mitochondrial fraction, and E. coli. A class of transfer RNA separable from the majority of transfer RNA by gel electrophoresis has been observed in E. coli and in the mitochondrial fraction of HeLa cells but not in HeLa cytoplasm. The size of this class of transfer RNA, as judged by its mobility on polyacrylamide gel, is intermediate between the major part of the transfer RNA and 5S RNA. It exists in part associated with the ribosomes, and its amino acid acceptor activity is different from the major class of transfer RNA.


Curent bibliography on allergy and applied immunology.


The negative control mechanism for E. coli DNA replication. Evidence is presented to show that the initiation of DNA replication in E. coli 555-7 requires synthesis of a protein whose production is correlated with total protein synthesis. Once replication is initiated, however, reinitiation will occur if all further protein synthesis is prevented; a small amount of protein synthesis is sufficient to prevent this unregulated reinitiation. This shows that the initiation of DNA replication is under negative control. A mechanism for the control of DNA replication is proposed; in this mechanism a replication repressor is synthesized periodically, while an antirepressor protein is synthesized continuously. Derepression of initiation results after sufficient accumulation of the antirepressor protein, and repression is re-established by repressor synthesis after the initiation of replication.


The dog as a symptomless carrier of Salmonella typhimurium.


Losses from botulism in Mallard duck and other water fowl.


The historical geography of malaria risk in Trinidad: three maps of change.


Mycoplasma suipneumoniae isolated in Denmark.


Acute respiratory failure in chronic obstructive pulmonary disease. I. Pathophysiology.


Human liver biopsy in P. falciparum and P. vivax malaria. A light and electron microscopy study.


The human foetal yolk sac. An ultrastructural study offour specimens.


The mineralization and hardness of the radular teeth of the limpet Patella vulgata L.


Fine structure of the pseudobranch of the flounder Paralichthys lethostigma. A description of a chloride-type cell and pseudobranch-type cell.


The ultrastructure of human secretory ameloblasts.


Discharge and restitution of secretory material in the rat parotid gland in response to isoproterenol.


Alfred Newton Richards scientist and man.


Mr. Gunning and his splint.

Mr. Gunning and his splint.


Acrylated arch bars.

Acrylated arch bars.


Panel discussion: proposed changes in the USP--microbiological aspects. I. Proposed changes in the USP sterility test.


Panel discussion: Proposed changes in the USP--Microbiological aspects. II. Biological indicators.


Panel discussion: Proposed changes in the USP--microbiological aspects. 3. Proposed microbial limit tests.


An enzymatic assay for octopamine and other beta-hydroxylated phenylethylamines.


Candidates and doctors theses for the years 1966-1967.


Escherichia coli serogroup 0115 isolated from patients with enteritis. Biochemical characteristics and experimental pathogenicity.


Present status of the treatment of schistosomiasis mansoni.


Fine structure of the cerebral ocelli of a sipunculid, Phascolosoma agassizii.


Fine structure of mammalian and avian pancreatic islets with special reference to D cells and nervous elements.


The ultrastructure of the nauplius eye of Sapphirina (Crustacea: Copepoda).


The ultrastructure of the liver in thyroid-fed rats.


Light- and electronmicroscopical observations on the saccus vasculosus of the rainbow trout.


Ultrastructural study on nuclear-cytoplasmic relationships in oocytes of the African lungfish, Protopterus aethiopicus. II. The microtubular apparatus of the nuclear envelope.


Cytochemical and ultrastructural studies of ribonucleoprotein containing structures in oocytes of Acheta domesticus.


Prostaglandins and cardiovascular diseases.


Experimental epidemiology: nutrition and infection.


Malondialdehyde and liver DNA.

Malondialdehyde and liver DNA.


Inhibition of prostaglandin synthesis by unsaturated fatty acids.


Protein synthesis in intestinal mucosa of rats deficienct in vitamin A.


Copper and the aorta.

Copper and the aorta.


The topical use of gentamicin in otorrhoea.


Trimethoprim-sulphamethoxazole in chronic bronchitis.


Scavenger of the faculty. Joshua Ward (1685-1761).


Dissociation of 70S ribosomes: some properties of the dissociating factor from Bacillus stearothermophilus and Escherichia coli. A protein factor which produces in vitro dissociation of 70S particles into 30S and 50S subunits has been obtained from Bacillus stearothermophilus and Escherichia coli. The factor could be extracted from ribosomes, polyribosomes, and S100 supernatant. The kinetics and temperature curve of the dissociation process in the B. stearothermophilus system have been studied and compared with the reaction in the E. coli system. No species specificity was observed when hybrid mixtures of ribosomes and dissociating factor from both bacteria were used. The wide variations of dissociating activity in cells at different stages of growth and the capacity of the liberated subunits to carry out polypeptide synthesis suggest that the dissociating factor has a physiological role.


The polyamine content of the tRNA of E. coli. A sensitive method of polyamine estimation has been adapted to the study of the organic cations of small amounts of nucleic acid. A procedure utilizing phenol extraction, alcohol precipitation, and separation on Sephadex G100 has been devised for the isolation of tRNA at low ionic strength. The procedure is applicable to the isolation of tRNA from liter batches of bacterial culture. With these methods we have examined the polyamines of tRNA isolated from polyauxotrophic strains of E. coli incubated under various physiological conditions and have found the following: (1) The tRNA from relaxed bacteria (TAU rel) harvested during exponential growth is heterogeneous with respect to polyamine content. Some portions of the population contain about one mole of spermidine per mole of tRNA. Some putrescine and an unknown amine are also present in low concentration. (2) After exponential TAU rel is incubated with thymine and uracil in the absence of arginine, the tRNA population is far more homogeneous with respect to polyamine content. The various fractions contain two moles of spermidine per mole of tRNA and a small amount of putrescine. (3) After exponential TAU rel is incubated in the absence of both arginine and uracil, the polyamine pattern of the tRNA resembles that isolated from exponential cells. (4) The tRNA from stringent bacterias harvested during exponential growth is heterogeneous with respect to polyamine distribution and some fractions contain relatively high concentrations of the unknown amine.


Repression-dependent alteration of an arginine enzyme in Escherichia coli. Treatment of susceptible Escherichia coli K12 derivatives with 0.4 M Mg(++) at 37 degrees , potentiated by L-arginine or L-canavanine, leads to alteration of acetylornithine delta-transaminase. The alteration, obtained in the absence of protein synthesis and reversible at 0 or 37 degrees , is manifested in extracts by lowered activity and modified substrate affinity behavior of the enzyme without gross changes in sedimentation properties. Cells grown under arginine repression are susceptible to the treatment; cells grown under genetic or steady-state physiological derepression are not. Transaminase synthesized during early derepression can be altered, although to progressively diminishing extents. Enzyme formed under steady-state derepression becomes alterable following transition to repression. The Mg(++) -dependent alteration can be thought to arise while the enzyme, arginine (or canavanine), and aporepressor are in contact, and to reflect a physiological process such as the participation of the enzyme in the repressive complex.


Ribonuclease V of escherichia coli. I. Dependence on ribosomes and translocation. A new RNase activity, tentatively named RNase V, was found in cell-free extracts of E. coli. This activity requires ribosomes, G and T factors, tRNA, K(+) or NH(4) (+), Mg(2+), GTP, and a sulfhydryl compound to degrade poly U, poly A, T4 phage mRNA, or E. coli mRNA. RNase V is specific for mRNA; it does not attack ribosomal RNA. It is inhibited by antibiotics that decrease breakdown of mRNA in vivo, such as chloramphenicol and streptomycin, and by such agents as 5'-beta, gamma-methylene-guanosine triphosphate, and fusidic acid, which inhibit ribosome-dependent GTPase and translocation of ribosomes along mRNA. The evidence suggests that RNase V is either an integral part of the ribosome or is tightly associated with it, and that it selectively degrades mRNA in intact cells.


DNA-dependent synthesis of RNA by Escherichia coli RNA polymerase: release and reinitiation of RNA chains from DNA templates. RNA synthesis in an in vitro RNA polymerase system at low ionic strength soon ceases, due to inhibition by accumulated RNA. Measurement of RNA chain initiation by the incorporation of gamma-(32)P-ATP and GTP with native T2 or T4 DNA as template shows that only one RNA chain is formed per molecule of enzyme added. In contrast, when the polymerase reaction is carried out in 10 mM Mg(++) and 0.2 M KCl, RNA synthesis proceeds nearly linearly for hours, resulting in a marked increase in accumulated RNA. Incorporation of gamma-(32)P-ATP also proceeds throughout the course of the reaction and the number of chains initiated per molecule of enzyme is increased severalfold. Most RNA chains formed are released from the DNA template as free RNA. Polymerase is released also in this process and acting catalytically reinitiates new chains. Rifampicin inhibits initiation of RNA synthesis and also blocks reinitiation of RNA chains without affecting growth of RNA chains already initiated.


Response of some subcortical structures to stimulation during stereotaxis, followed by means of correlation analysis.


Common problems of methodology of the physician and the computer.


Rapidly-growing mycobacteria. Susceptibility to bacteriophages, reactions in the amidase test, production of acid from carbohydrates, and growth at various temperatures.


Sir Henry Dale, M. D. (1875-1968).

Sir Henry Dale, M. D. (1875-1968).


Surgery of the orbit.

Surgery of the orbit.


Charles W. Mayo, M. D. (1898-1968).

Charles W. Mayo, M. D. (1898-1968).


Activity of chloramphenicol against Escherichia coli circulating in the blood and tissues of immunized dogs.


Secondary hyperparathyroidism in chronic renal failure. The clinical spectrum in uremia, during hemodialysis, and after renal transplantation.


The effect of surrounding bacterial contamination and infection upon the healing of colon wounds in dogs.


Isolation of a gene.

Isolation of a gene.


The development of DNA containing nuclear bridges in human cells.


Changing faecal population of Escherichia coli in hospital medical patients. Specimens of faeces were obtained at weekly intervals for one year from patients in a female medical ward and Escherichia coli present were typed. The faecal E. coli population of the patients was constantly changing. No serotypes of E. coli were dominant, but on 31 occasions during the year small clusters of patients carried the same type.


Appetite and weight gain in children: a double-blind trial using cyproheptadine and methandrostenolone.


Charles A. Berry M.D.

Charles A. Berry M.D.


Some properties of chick ultimobranchial calcitonin.


An assessment of Bovril as a stimulus to the secretion of human growth hormone.


The calcitonin story.

The calcitonin story.


Pain control and patient management in dentistry--a review of current intravenous technics.


More retentive complete dentures.

More retentive complete dentures.


Editorial. Harold Hillenbrand, DDS.

Editorial. Harold Hillenbrand, DDS.


Harold Hillenbrand, DDS, Executive Director Emeritus, American Dental Association.


Art and artist.

Art and artist.


Doctor Watson assumes top ADA post January 1.


Doctor Hillenbrand receives Callahan Memorial Award.


Two oral surgeons named to receive Gies Award.


T. F. McBride receives 1969 editors award.


Dr. Washburn president-elect of medical library group.


Development of pharmacy in Latin America.


Laboratory research in food allergy. I. Food allergens.


Auto-allergies of the central nervous system: a review.


The chromatographic purification of human kappa-casein.


The Abraham Flexner Award. John M. Russell.


The Borden Award. Abraham White.

The Borden Award. Abraham White.


A course in biomedical computing applications.


John Chapman (1822-1894), sponsor of George Eliot.


Cardiac transplantation in man: its therapeutic and other importance.


In memoriam. Robert K. Lippmann, M. D., 91-1969.


Subclinical renal infection in pregnancy; pathogenesis, the organisms and the drugs of choice in its treatment.


Kenneth Barron Fraser.

Kenneth Barron Fraser.


The psychiatrist looks at medical progress.


Bulking agents in foods.

Bulking agents in foods.


Liver methylmalonate and CoA concentrations in sheep deficient in vitamin B 12.


Flap repair following exenteration of the orbit.


Disturbances of gas metabolism in patients with occlusive cerebrovascular disease.


Neuropathology of intracranial haemorrhage.


Echo-encephalography and cerebral circulation.


Arterial vertebro-basilar aneurysms.

Arterial vertebro-basilar aneurysms.


Arteriovenous aneurysms of the posterior fossa.


Biodegradation kinetics of 2,4-dichlorophenoxyacetic acid by aquatic microorganisms.


Effects of processing on pesticides in food.


Samuel Sharp "on concussions of the brain" (1754).


Lithotomy and lithotomists: progress in wound management from Franco to Lister.


Nonspecific ulcerative colitis.

Nonspecific ulcerative colitis.


Role of thromboendarterectomy and bypass graft in occlusive vascular disorders.


Thracian origin of Asclepius, god of healing.


Studies on vibriosis in an A.I. stud.

Studies on vibriosis in an A.I. stud.


Salmonellosis from chickens prepared in commercial rotisseries: report of an outbreak.


Fate of 3H-ribose in the rat as detected by radioautography.


Actinomycin D and amphibian cells in vitro: effects on monolayers, postmitotic nucleolar reconstruction and nucleolar emptying at different temperatures.


Pudendal neurectomy in management of neurogenic bladder in myelomeningocele.


Sucking and swallowing difficulties in infancy: diagnostic problem of dysphagia.


Effect of acute hypoxia on blood pressure and electroencephalogram of newborn babies.


Deacylation of acylamino compounds other than penicillins by the cell-bound penicillin acylase of Escherichia coli. 1. The action of the penicillin acylase enzyme of Escherichia coli N.C.I.B. 8743 on non-penicillin substrates suggests that the enzyme is an amidohydrolase. 2. The rates of hydrolysis for a small group of penicillins closely parallel those for a corresponding series of N-acylglycines. 3. For a series of E. coli strains, ability to cause rapid hydrolysis of phenylacetylglycine is correlated with ability to hydrolyse benzylpenicillin. 4. Amides and N-acylglycines are hydrolysed to the corresponding acids. The phenylacetyl group is hydrolysed most readily. Benzamide and beta-phenylpropionamide are not substrates. In a series of aliphatic acylglycines only valeryl- and hexanoyl-glycine are substrates. 5. Acylated l- but not d-alpha-amino acids are hydrolysed. d-alpha-Hydroxyphenylacetamide is a better substrate than the l compound.


Factors affecting the synthesis of ampicillin and hydroxypenicillins by the cell-bound penicillin acylase of Escherichia coli. 1. The effect of pH, temperature, reactant concentration and reaction time has been investigated for the synthesis of benzylpenicillin, dl-alpha-hydroxybenzylpenicillin and d-alpha-aminobenzylpenicillin from 6-aminopenicillanic acid by the penicillin acylase of Escherichia coli. 2. Synthesis of penicillins from carboxylic acids proceeds most rapidly at pH5; with amides the optimum pH is higher (6-7) but the reverse reaction rapidly sets in. This can be counteracted by lowering the pH or adding more amide. Optimum temperatures are 35-40 degrees . 3. Most rapid synthesis of penicillin was obtained with the N-acylglycine and methyl ester derivatives of carboxylic acids. Increasing the amide/6-APA ratio above 1:1 raised the rate of synthesis of penicillins. 4. Preferential synthesis of d-alpha-hydroxybenzylpenicillin takes place in a reaction mixture containing dl-mandelic acid. 5. From d- and l-mandelamide, d- and l-alpha-hydroxybenzylpenicillins were prepared, the former being more bioactive than the latter. p-Hydroxy- and 3,4-dihydroxybenzylpenicillins were also prepared, the latter being more active against some Gram-negative bacteria than benzylpenicillin.


Regulation of synthesis of glutamate dehydrogenase and glutamine synthetase in micro-organisms. 1. Aspergillus nidulans, Neurospora crassa and Escherichia coli were grown on media containing a range of concentrations of nitrate, or ammonia, or urea, or l-glutamate, or l-glutamine as the sole source of nitrogen and the glutamate dehydrogenate and glutamine synthetase of the cells measured. 2. Aspergillus, Neurospora and Escherichia coli cells, grown on l-glutamate or on high concentrations of ammonia or on high concentrations of urea, possessed low glutamate dehydrogenase activity compared with cells grown on other nitrogen sources. 3. Aspergillus, Neurospora and Escherichia coli cells grown on l-glutamate possessed high glutamine synthetase activity compared with cells grown on other nitrogen sources. 4. The hypothesis is proposed that in Aspergillus, Neurospora and Escherichia colil-glutamate represses the synthesis of glutamate dehydrogenase and l-glutamine represses the synthesis of glutamine synthetase. 5. A comparison of the glutamine-synthesizing activity and the gamma-glutamyltransferase activity of glutamine synthetase in Aspergillus and Neurospora gave no indication that these fungi produce different forms of glutamine synthetase when grown on ammonia or l-glutamate as nitrogen sources.


Studies on the binding of phenylalanyl transfer RNA to rat-liver ribosomes.


Reactions of N-acetylphenylalanyl transfer RNA with rat-liver ribosomes.


A study of factors influencing the retention of DNA by membrane filters.


Studies on the kinetics of peptidyl transfer RNA translocase from rat liver.


The Escherichia coli B restriction endonuclease.


Isolation of nuclei from Euglena gracilis.


Infrared spectra of transfer RNA's. II. Formylmethionine transfer RNA from Escherichia coli in aqueous solution.


Binding of 5-S ribosomal RNA to unfolded 50-S ribosomes in Escherichia coli.


Pool size and specific activity of UTP in isoproterenol-stimulated salivary glands.


Synthesis of peptidyl aminoacyl transfer RNA: a chemical method for the purification of transfer RNA's.


Is secretin enterogastrone?

Is secretin enterogastrone?


Autograft rejection, temporary transplantation of autografts to allogeneic and xenogeneic hosts within and without diffusion chambers.


Influence of age on calcification in homologous transplants in rats.


Shwartzman phenomenon without endotoxin preparation.


Anew method of examining the stimulatory effect of Serratia marcescens endotoxin on non-specific resistance to infection.


Filamentous growth of some Mycoplasma species of man.


Anterolateral operations for fractures and dislocations in the middle and lower parts of the cervical spine. Report of a series of forty-seven cases.


Homocystinuria. A report of two cases and review of the literature.


Bone findings in chronic granulomatous disease of childhood. A genetic abnormality of leukocyte function.


Replacement of a cervical vertebral body for aneurysmal cyst. A case report.


Metacarpal lengthening: a case report.

Metacarpal lengthening: a case report.


Instability of a missense suppressor resulting from a duplication of genetic material.


Synthesis and assembly of ribosomal protein into 50 s subunits during recovery from chloramphenicol treatment.


The process of infection with bacteriophage phiX174. XXV. Studies with bacteriophage phiX174 mutants blocked in progeny replicative form DNA synthesis.


The oxygen uptake and pulmonary blood flow during resuscitation from asphyxia in foetal and adult sheep. 1. The foetuses of twenty-seven Welsh ewes and eight Clun Forest ewes were delivered by Caesarian section at an approximate gestational age of 140 days, maintaining the umbilical circulation. They were asphyxiated by clamping the umbilical cord, and resuscitated with IPPV. Oxygen consumption, heart rate, and blood pressures were recorded, and in one series of eight, blood flow through the left lung was recorded whilst IPPV was applied to this lung only.2. The hypothesis that oxygen consumption is abnormally low during resuscitation of the new-born from extreme asphyxia because of limited blood flow, was supported.3. Adult ewes were also asphyxiated and in a series of eight a quite different pattern of behaviour was observed, due to very rapid and complete recovery of the circulation.4. Observations on the pulmonary vascular resistance suggest that complete vasomotor paralysis occurs during extreme asphyxia, so that resistance falls to a near-minimum level for unexpanded lung. Expansion of the lung produced a large fall in resistance which appeared to be due to the introduction of gas into the alveolar spaces, lessening the resistance to flow of the capillaries.


Bacteriology of diarrhoea in Sudanese children.


Sir Archibald Garrod's conception of chemical individuality: a modern appreciation.


Observations on the growth of Eimeria tenella in cultured cells from the parasitized chorioallantoic membranes of the developing chick embryo.


Differences between streptococcal infections of the throat and of the skin (second of two parts).


Development of research on the nutrition of the population of Slovakia.


Three-dimensional observation of the inner ear with the scanning electron microscope.


Prolonged survival of allo- and xeno-grafts of skin by means of an antitumoral compound: the 1-asparaginase.


On the specificity of the alcoholic, acidic silver nitrate reagent for the histochemical localization of ascorbic acid.


The elution patterns of formaldehyde, glutaraldehyde, glyoxal and alpha-hydroxyadipaldehyde from sephadex G-10 and their significance for tissue fixation.


Studies on the responses of children during otitis media chronica.


Dr. Harvey Cushing, M.D. Memoir for the centenary year of his birth.


Some recent developments in virology.

Some recent developments in virology.


Some effects of gamma-radiation on Trypanosoma cruzi, culture and blood forms.


Recent advances in clinical medicine.

Recent advances in clinical medicine.


The XYY syndrome.

The XYY syndrome.


ANA's new executive director states her views.


Color in sunglass lenses.

Color in sunglass lenses.


The typoscope by Charles F. Prentice.

The typoscope by Charles F. Prentice.


Lipomatous invasion of the spinal cord associated with spinal dysraphism: myelographic evaluation.


The chronometric bioassay: a microbiological assay for antibiotics based on continuous dilution.


Comparative studies of reagents used in automated CCA testing of influenza virus.


Chemotherapy of the drug-resistant malarias.


Quantifying epithelial healing of the cornea in vivo.


On the quantitation and analysis of bile pigments in amniotic fluid.


America's role in world nutrition.

America's role in world nutrition.


Forensic odontology. Identification by comparison.


George Cunningham: the man and his message.


Clinical measurements relevant to the assessment of hypoxia.


Measurement of inspired and expired oxygen and carbon dioxide.


Immunosuppressive action of L-asparaginase in mice.


A random sample from Wales. IV. Circulating gastric and thyroid antibodies and antinuclear factor.


Physiology of the choroid plexus and experimental studies of the cerebrospinal fluid: a review.


A simple "curve-fit" method of calculating area under the terminal downslope of indicator dilution curves.


Pulmonary intra- and extravascular fluid volume changes with exercise.


Correction of distortion in dye dilution curves due to sampling systems.


The reduction of indoles and related compounds.


The structure and function of the intercalated disc in vertebrate cardiac muscle.


Physiological and pharmacological properties of Limulus heart.


RNA induced anti-kidney auto-antibodies.


Bibliography of M. N. Smith-Petersen.

Bibliography of M. N. Smith-Petersen.


Jest appreciation and interaction in leaderless groups.


Group counseling with underachievers: a review and evaluation of methodology.


The effects of group and individual therapy on sociometric choice of disturbed, institutionalized adolescents.


The effects of propranolol in essential circulatory hyperkinesis.


Penetration of antibiotics into the cerebro-spinal fluid in inflammatory conditions. 3. Gentamicin.


Toxicologic appraisal of particulate matter, oxides of sulfur, and sulfuric acid.


Toxicological appraisal of lead in relation to the tolerable concentration in the ambient air.


Editorial. The Pentagon's health chief.

Editorial. The Pentagon's health chief.


Toxicological appraisal of carbon monoxide.


Erosion-abrasion.

Erosion-abrasion.


Bibliography: selected additional references on family planning.


Richard Morton (1637-1698).

Richard Morton (1637-1698).


Who is this doctor?

Who is this doctor?


Review of the pharmacologic and therapeutic effects of diisopropylammonium dichloroacetate (DIPA).


To Rudolf Nissen's 70th birthday.

To Rudolf Nissen's 70th birthday.


Second introduction of the Rudolph Virchow lecturer.


Some considerations in the therapy of pineal tumors.


The pharmacological importance of the uptake mechanism for sympathomimetic amines.


The influence of Tris-HCl on the biosynthesis of polyphenylalanine.


Infection of tobacco mesophyll protoplasts by tobacco mosaic virus ribonucleic acid.


Chromosome mobilization in rec-merodiploids of Escherichia coli K12 following infection with bacteriophage lambda.


The genetics and physiology of bacteriophage T7.


T7-directed protein synthesis.

T7-directed protein synthesis.


Specific surface antigen in Shope papilloma cells.


Host-controlled restriction and modification of filamentous 1- and F-specific bacteriophages.


A quantitative, sensitive assay for defective lambda particles.


The virulence of some Escherichia coli strains for larvae of Galleria mellonella L.


Evaluation of the earlobe in leprosy. A clinical and histopathological study.


The role of histology in the study of leprosy.


Peter Flesch, MD, PhD 1915-1969.

Peter Flesch, MD, PhD 1915-1969.


Action of propranolol on left ventriclar contraction in aortic stenosis when a fall in heart rate is prevented by atropine.


Purkynĕ's (Purkinje's) muscle fibres in the heart.


Effect of posture on cardiac output after myocardial infarction.


George Edgar Septimus Ward, 1888-1969.

George Edgar Septimus Ward, 1888-1969.


Camille Lian, 1882-1969.

Camille Lian, 1882-1969.


The surgical treatment of cavernous haemangiomas of the hand.


The treatment of giant hairy pigmented naevi of the face.


The repair of notches of the alar margin.


Operative treatment of deep burns of the scalp.


Primary bone grafting in the treatment of cleft lip and palate with special reference to alveolar collapse.


Anaesthesia for cleft palate surgery. A comparative study of anaesthetic methods.


A flap for naso-labial defects, or, "save the dog-ear".


Healing of donor sites of split skin grafts. An experimental study in pigs.


Per via naturalis following reconstruction of the vagina.


Squamous cell carcinoma occurring in chronic perianal pyoderma.


Self-medication of herpes zoster with an arsenic paste. A case report.


Experimental skin grafts in England, 1663-64.


Observations on the decreasing frequency of multiply resistant strains of Shigella sonnei.


Use of Pankhurst tubes to assay acetylene reduction by facultative and anaerobic nitrogen-fixing bacteria.


Some ultrastructural and biochemical characteristics of tumor mitochondria isolated in albumin-containing media.


The HFH-18 mouse melanoma in roller tube, chamber, and circumfusion system cultures.


On the mechanism of sensitivity to leucine in the case of "leucine sensitive hypoglycemia". II. The hypoglycemic affect of alpha-ketobutyric acid in the case of "leucine sensitive hypoglycemia".


A modified coated-charcoal immunoassay for measuring insulin release in vitro.


Physical separation of hemopoietic stem cells from cells forming colonies in culture.


A new cellular source for establishment of the karyotype in the sow.


The early lesion of experimental allergic orchitis in guinea pigs: an immunological correlation.


An attempt to establish experimental dysenteric bacilli cystitis.


Experimental salmonellosis. Immunizing effect of live vaccine prepared from various mutants of Salmonella having different cell wall polysaccharides.


Unstable mutants of R factor.

Unstable mutants of R factor.


A simple method ("plate-in-bottle method") for the cultivation of fastidious anaerobes.


Isolation of an E. coli strain with a mutation affecting DNA polymerase.


Genetic analysis of an E. coli strain with a mutation affecting DNA polymerase.


Termination factor for RNA synthesis.

Termination factor for RNA synthesis.


Salt appetite.

Salt appetite.


A medical emergency treated by anti-mitotic drugs.


The surgical treatment of acute massive pulmonary embolism.


Xanthogranulomatous pyelonephritis.

Xanthogranulomatous pyelonephritis.


Treatment of intoxication with combinations of drugs and management of the associated shock.


Serological response in cattle to East Coast fever (Theileria parva infection) as measured by the indirect fluorescent antibody test.


Effects of injection of enterotoxin, endotoxin or live culture of Escherichia coli into the small intestine of pigs.


Biographical sketch of Josef Igersheimer.


Knowledge of the pupillary reactions in Argyll Robertson's time.


The current status of ocular syphilis.

The current status of ocular syphilis.


Implications of spiral forms in the eye.


The treatment of syphilis.

The treatment of syphilis.


The fine structure of the collar cells in the optic tentacles of Helix aspersa.


A morphological and cytochemical study of sarcoplasmic reticulum and T system of fish extraocular muscle.


Further studies on the ring canal system of the ovarian cystocytes of Drosophila melanogaster.


Stereotaxic surgery using a television guidance system. II. Percutaneous cordotomy.


Studies on myxovirus pyrogen. (II). Some factors affecting fever tolerance in rabbits.


Tryptophan pyrrolase--a biochemical factor in depressive illness?


Edward Mapother and the making of the Maudsley Hospital.


The response to treatment of individual patients in a drug trial.


A controlled physiological, clinical and psychological evaluation of chlordiazepoxide.


Nitrazepam (Mogadon) as a sleep-inducing agent. An analysis based on a double-blind comparison with phenobarbitone.


Comparative effects of propranolol and chlordiazepoxide in anxiety states.


The effect of glucose on the endocrine pancreas ultrastructure of the frog (Rana ridibunda).


Hydroxyzine hydrochloride in the management of children with behaviour problems.


A controlled trial of a protein-free liver extract in the treatment of chronic liver disease. A double-blind trial of a protein-free liver extract (Ripason) which was administered both orally and intramuscularly is described in 20 patients with stable cirrhosis. Control and treated groups were similar in composition except for age. Both groups showed some symptomatic improvement during the trial. Fewer patients were recorded as worse on Ripason but no significant changes in body weight, haemoglobin, or liver function tests could be detected.


Diarrhoeal disease of infancy in Kabul.

Diarrhoeal disease of infancy in Kabul.


Partial purification and some properties of methionine transfer RNA of baker's yeast.


Rapidly reversible dissociation of polysomes in Escherichia coli by n-butanol.


Influence of epinephrine-- propanolol infusions on growth hormone release in normal and hypopituitary subjects.


Hypothalamic-pituitary-adrenal axis in methadone-treated heroin addicts.


Evidence for 3-alpha, 17,21-trihydroxy-5-alpha-pregnane-ll,20-dione ("ATHE"), a metabolite of cortisol in urine of infants.


Initiation of DNA replication cycles in Escherichia coli following DNA synthesis inhibition.


Mutagenic effect of sensitized irradiation of bacteriophage T4.


Proflavin-induced mutations in the L-arabinose operon of Escherichia coli. II. Enzyme analyses of the mutants.


An L-arabinose binding protein and arabinose permeation in Escherichia coli.


Induction of the L-arabinose operon.

Induction of the L-arabinose operon.


Proflavin-induced mutations in the L-arabinose operon of Escherichia coli. I. Production and genetic analyses of such mutations.


Ribonucleoside diphosphate reductase. Formation of active and inactive complexes of proteins B1 and B2.


Role of effector binding in allosteric control of ribonucleoside diphosphate reductase.


Effect of acridine orange on sex factor multiplication in Escherichia coli.


Carotid vertebral trauma.

Carotid vertebral trauma.


Clinical diagnosis of the "latent" ischemic heart failure.


Cushing's disease in a boy: serial steroid and growth hormone studies.


Humoral antibodies in renal allotransplantation in man.


Prevention of hyperuricemia by allopurinol in hypertensive patients treated with chlorothiazide.


Clinically important variants of human hemoglobin.


The ecology of r factors.

The ecology of r factors.


Recent advances in the physiology and biochemistry of plasmodia.


The response of BHK21 cells to infection with type 12 adenovirus. 1. Cell killing and T antigen synthesis as correlated viral genome functions.


The response of BHK21 cells to infection with type 12 adenovirus. II. Relationship of virus-stimulated DNA synthesis to other viral functions.


Replication of bacteriophage ribonucleic acid: alterations in polyribosome patterns in Escherichia coli infected with amber mutants of bacteriophage R17.


Excision of lambda prophage following induction.


Genetic map of bacteriophage P2.

Genetic map of bacteriophage P2.


Multiple recombination mechanisms in bacteriophage P2.


Attachment of prophage P2: gene order at different host chromosomal sites.


Bacteriophage T4-induced deoxycytidine triphosphate-deoxyuridine triphosphate nucleotidohydrolase: its properties and its role during phage infection of Escherichia coli.


Defective thermal induction of a noninducible bacteriophage.


A simple method of preserving ocular tissue for scanning electron microscopy.


Adenovirus type 8 antigens and interferon induction.


In memoriam--S. Bernard Wortis, 1904-1969.


The history of psychiatry: its relevance for the psychiatrist.


Experimental deficiency of gastric "mucus barrier." Its effect on gastric secretion and on the formation of acute gastric ulcer in rats infused with histamine for 24 hours.


A controlled study of nortriptyline and imipramine.


The evolution of the mental status--past and future.


Facial scar deformities and their management.


Heat stroke.

Heat stroke.


Safeguards in the use of topical mafenide (Sulfamylon) in burned patients.


Treatment of varicose veins by continuous circumsuturing.


Heart transplantation. The effect of pretreatment with antilymphocyte serum.


The use of chlorhexidine antisepsis in contaminated surgical wounds.


Trans-hepatic vascular resistance during prolonged isolated liver perfusions.


An experimental trial of gastric plication as a means of weight reduction in the rat.


In memoriam. Sir Marriott Fawkner Nicholls, C.B.E., M.A., M.Chir. (Cantab.), F.R.C.S. (Eng.), (1898-1969).


A technique for detecting Treponema pallidum through the use of membrane filters and immunofluorescence staining.


Immunogenic properties of the protein component of Treponema pallidum.


Artificial immunization of rabbits against syphilis. I. Effect of increasing doses of treponemes given by the intramuscular route.


Trichomonas vaginalis. I. Survival in solid Stuart's medium.


The heart in medicine.

The heart in medicine.


Rembrandt's "eye operation".

Rembrandt's "eye operation".


University of California School of Medicine; San Francisco Medical Center.


Assay of serum vitamin B12 levels by saturation analysis.


Intramuscular diazepam as anti-anxiety agent in SCC-modified EST.


Learning disorders as a school health problem. Neurological and psychiatric aspects. Broadened concepts of intellectual functions have shown that many varieties of mental subnormality may be preventable or subject to improvement with proper treatment. Many types of neurologic dysfunction are accompanied by learning disorders based on specific intellectual deficits.A more refined delineation of the higher cerebral functions of each child with a learning disorder provides the basis for improved specific remedial educational techniques. Such detailed assessment of higher functions of the nervous system can be greatly enhanced by the appropriate special evaluations carried out by well trained psychologists, speech pathologists and educational consultants, working in cooperation with physicians. The varieties of adjustment problems of children and emotional impact of a learning disorder should be recognized as early as possible and treated appropriately. Motor and perceptual-motor therapies may have limited value in some cases but may be harmful if indiscriminately applied. Psychotropic drugs have a relatively limited place in the management of learning disorders but may be immensely valuable in some cases by helping to control specific behavior problems which interfere with learning processes. Physicians have a major responsibility to provide help and leadership in dealing with learning disorders.


The importance of airborne algae and protozoa.


Nitrogen metabolism in ruminants: rate of ruminal ammonia production and nitrogen utilization by ruminants--a review.


Spina bifida and anencephaly: A problem in genetic-environmental interaction.


Manipulation of indications of disturbed thinking in normal subjects.


Robert Allen Cooley, 1873-1968.

Robert Allen Cooley, 1873-1968.


Leonard Haseman, 1884-1969.

Leonard Haseman, 1884-1969.


Oris Trigue Robertson, 1905-1969.

Oris Trigue Robertson, 1905-1969.


The electron microscope and biological electron microscopy.


The history of Flint-Goodridge Hospital of Dillard University.


Control of enuresis in disturbed adolescent boys.


John H. Talbott, MD.

John H. Talbott, MD.


Microscopic studies on crystals in skeletal muscle from two cases of xanthinuria.


Genetic control of hexose phosphate uptake by Escherichia coli.


Multiple molecular species of circular R-factor DNA isolated from Escherichia coli.


Effect of a murine leukaemia virus on "backgroud" antibody plaque-forming cells to sheep erythrocytes and E. coli antigen.


The pharmacology and biochemistry of N-oxides.


The pathological anatomy of the nephrotic syndrome.


On becoming an American.

On becoming an American.


Gastro-enteritis in infancy.

Gastro-enteritis in infancy.


James Edward Fitzgerald. In memoriam.

James Edward Fitzgerald. In memoriam.


Leonard Samuel Fosdick, Ph.D. 1903-1969.


British X-ray patents, 1896-1900.

British X-ray patents, 1896-1900.


Escherichia coli enterotoxin.

Escherichia coli enterotoxin.


Veterinary pathology: retrospect and prospect.


Indane and indene derivatives of biological interest.


Biochemistry and pharmacology of methyldopa and some related structures.


Anaerobic indicators: a review.

Anaerobic indicators: a review.


Timely topics in microbiology: micrococcaceae and streptococci.


Phenolic substances in grapes and wine, and their significance.


Urinary output and plasma levels of antidiuretic hormone during intermittent positive-pressure breathing in the dog.


Cerebral circulatory response to acute brain disease: Implications for anesthetic practice.


A simple valve for maintenance of positive airway pressure during deliberate hypotension.


Bupivacaine hydrochloride: laboratory and clinical studies.


Standardized interpretation of laboratory data.


Normal values in medicine.

Normal values in medicine.


Occlusion of the vertebral arteries. Causing transient basilar symptoms.


Number of Purkinje's cells after diphenylhydantoin intoxication in pigs.


The lens and vitreous.

The lens and vitreous.


Clinical experience of 'Bisolvon' in 220 cases of chest disease.


Post-operative angiography in renal homotransplantation.


The radiological demonstration of urological complications in renal transplantation.


The radiological size of renal transplants--a retrospective study.


Measurement of cardiac output with indium 113m-labelled transferrin.


Guidelines to management of some respiratory problems.


EB virus antibody at different ages.

EB virus antibody at different ages. In relation to the study of infectious mononucleosis a survey of the prevalence of antibody to EB virus was made on the sera of persons of varying age from several different areas in England by the indirect immunofluorescence technique. About half those tested were found to have acquired antibody by the age of 4 years and there was a further significant increase in the proportion of those positive between the ages of 15 and 24 years. The finding of seroconversion in two patients who developed infectious mononucleosis provides further support for an association between EB virus and this condition.


The role of posture in anesthesia.

The role of posture in anesthesia.


Postanesthetic shivering.

Postanesthetic shivering.


The treatment of classical hemophilia with factor 8 concentrates.


Complications of the postoperative period.


Obesity and anesthesia.

Obesity and anesthesia.


Advances in cytologic screening.

Advances in cytologic screening.