Strand-specific transfer of donor DNA during conjugation in E. coli. After conjugation of E. coli, only the preformed donor DNA strand with a leading 5' end can be recovered from the recipient. DNA that was transferred to recipients from radioactive-labeled donor strains lysogenic for lambda bacteriophage was recovered as radioactive material in mature phage particles. The radioactivity was recovered in strand l when gene transfer was in the order gal-lambda-bio, and in strand r when the order of transfer was bio-lambda-gal. The assignment of the 5' end as the origin of the transferred strand follows directly from the known orientation of prophage lambda in the chromosome.


A factor that stimulates RNA synthesis by purified RNA polymerase. A protein factor, partially purified from E. coli, can stimulate some 30- to 50-fold the transcription of DNA by purified RNA polymerase. Evidence is presented suggesting that this stimulatory factor (designated M factor) exerts its effect before the RNA synthesis is initiated. The M factor is prepared from a crude ribosome fraction, but it does not require the presence of ribosomes for full activity.


Release of transfer RNA during peptide chain elongation. In amino acid polymerization, the tRNA donating its peptidyl moiety to the neighboring aminoacyl-tRNA must be released from the ribosome for further growth of the polypeptide chain. It was not known at what stage of peptide elongation this tRNA is released. To study this question, we prepared ac-C(14)-Phe-H(3)-tRNA using H(3)-tRNA isolated from E. coli strain 15THU grown in the presence of H(3)-uracil, and followed the fate of the H(3)-tRNA during chain growth. Our results indicate that donor tRNA is released during the translocation step, mediated by the soluble factor G and GTP.


Peptide chain termination. 3. Stimulation of in vitro termination. Throughout extensive purification, the release factors R(1) and R(2) each behave as a single molecular species with alternate codon recognition (R(1), UAA or UAG; R(2), UAA or UGA). The release of f[(3)H]methionine from f[(3)H]-Met-tRNA.AUG.ribosome complex requires R factor and terminator codon and does not appear to require tRNA or transfer factors T and G. Purification of the components of the release assay has enabled identification of a protein factor S in the 55-80 per cent ammonium sulfate fraction of E. coli B supernatant fraction which stimulates the rate but not the extent of release dependent upon R factor and appropriate termination codon. The S factor has properties similar to T, but further purification is required to determine the nature and function of S in peptide chain termination.


Polypeptide chain initiation in E. coli: sulfhydryl groups and the function of initiation factor F2. The activity of the chain initiation factor F(2) in promoting the messenger-dependent binding of formylmethionyl-transfer RNA (fMet approximately tRNA(f)) to purified E. coli ribosomes is inhibited by sulfhydryl-binding reagents, such as N-ethylmaleimide or p-hydroxymercuribenzoate, but prior incubation with guanosine triphosphate (GTP) or with ribosomes largely prevents this inhibition. The effect of GTP suggests that it forms a complex with F(2) whereby "active" sulfhydryl groups become sheltered. Experiments on the time course of the binding reaction, with and without preincubation of F(2) with GTP, and gel filtration experiments with (3)H-labeled GTP lend support to this suggestion.


Restriction of nonglucosylated T-even bacteriophages by prophage P1.


The mechanism of restriction of bacteriophage lambda in Escherichia coli strains: demonstration of an in vivo requirement for S-adenosylmethionine.


Bibliography of scientific publications by the staff on the Charles University medical faculty in Prague in 1966.


Factors affecting obesity.

Factors affecting obesity.


Presentation of the distinguished service scroll to B. Edwin Erikson.


A dynamic pathology of arteriosclerosis.


Excellence in nursing.

Excellence in nursing.


Effects of alkylating chemicals on reproductive cells.


The genetic effects of the biological alkylating agents with reference to pesticides.


VI. Biochemical and pathological effects. Organochlorine insecticides and the stimulation of liver microsome enzymes.


New naturally occurring amino acids.

New naturally occurring amino acids.


Metabolism of fatty acids.

Metabolism of fatty acids.


DNA modification and restriction.

DNA modification and restriction.


Cell transformation and the genesis of cancer.


John Hunter and after: renal calculi and cancer of the bladder.


Central and peripheral vascular effects during cigarette smoking.


5-hydroxytryptamine metabolism in early embryogenesis, and the development of brain and retinal tissues. A review.


The Rev. Samuel Warneford, M.A., LL.D. (1763-1855).


[Stereotaxic thalamotomy for pain relief].


SS disease, SC disease, and CC disease--obstetric considerations and treatment.


Postoperative pelvic infections.

Postoperative pelvic infections.


Laboratory approach to diagnosis of hemoglobin abnormalities.


The significance of the dose of antien in immunity and tolerance.


The herpesviruses--a biochemical definition of the group.


Recent problems in the genetic code.

Recent problems in the genetic code.


Growth patterns of antibody-forming cell populations.


Value of preoperative electrodiagnosis in assessment of locomotor function of lower limbs in spina bifida cystica.


Role of pharmacological factors in the evaluation of anticonvulsant drugs.


Characterization of a glutamine synthetase b activating (deadenylylating) enzyme system in Escherichia coli.


Aflatoxin in two instalments. I.

Aflatoxin in two instalments. I.


Lead astray?

Lead astray?


Puerperal sepsis and endotoxin shock.

Puerperal sepsis and endotoxin shock.


Adaptation to extrauterine life.

Adaptation to extrauterine life.


The effects of short-term corticosteroid therapy on the symptoms of infectious mononucleosis pharyngotonsillitis: a double-blind study.


The untoward reaction of the horse in injection of antigenic substances.


Fatty-acid composition of Candida utilis as affected by growth temperature and dissolved-oxygen tension. Analyses were made of the fatty-acid composition of Candida utilis NCYC 321 grown in a chemostat at a dilution rate (equal to growth rate) of 0.1 hr(-1) and at temperatures in the range of 30 to 15 C and dissolved oxygen tensions between 75 and <1 mm of Hg. Cells grown under glucose limitation or NH(4) (+) limitation contained mainly C(16:0), C(16:1), C(18:0), C(18:1), C(18:2), and C(18:3) acids as detected by gas-liquid chromatography of methyl esters of the acids from lipids extracted with chloroform-methanol. The relative proportions of these acids varied with the growth temperature and the dissolved-oxygen tension in the culture. A decrease in growth temperature from 30 to 20 C led to an increased synthesis of unsaturated acids in cells grown under either limitation at a fixed-oxygen tension in the range of 75 to 5 mm of Hg. In cultures with a dissolved-oxygen tension of 1 and <1 mm of Hg, a further decrease in temperature to 15 C caused an increased synthesis of unsaturated fatty acids. A decrease in dissolved-oxygen tension led to a diminished synthesis of unsaturated fatty acids in cells grown at a fixed temperature under either limitation. Cells grown at a fixed temperature under glucose limitation synthesized a greater proportion of C(16) acids at the expense of C(18) acids as the dissolved oxygen tension was decreased from 75 to <1 mm of Hg. A preferential synthesis of C(16) acids also occurred as the growth temperature was decreased from 30 to 15 C in cells grown under glucose limitation at a fixed-oxygen tension. The same effect was observed in cells grown under NH(4) (+) limitation when the temperature was lowered from 30 to 20 C; but when the temperature was decreased further to 15 C, the cells synthesized a slightly greater proportion of C(18) acids. Synthesis of a large proportion of C(16) acids was accompanied by an excretion of pyruvate, and occasionally traces of 2-ketoglutarate, and an increased intracellular accumulation of certain amino acids.


Amino acid differences in a 30S ribosomal protein from two strains of Escherichia coli. The 30S ribosomal proteins of the K-12 and B strains of Escherichia coli differ in at least one protein component. This component, which is allelic in the two strains, has been isolated from both organisms. Amino acid analyses show that the protein from strain B contains between 20 and 28 more amino acids than does the analogue protein from strain K-12.


Intracellular protein breakdown in a thermophile. Protein breakdown of 5 to 7% per hr was found in nitrogen-starved cells of an unclassified prototrophic thermophilic bacillus; a similar protein-breakdown rate (6.5% per hr) was found in resting cells of Escherichia coli. In the thermophile, the rate of protein breakdown was markedly influenced by the temperature; it was maximal between 45 and 55 C, and it decreased considerably at 35 and 75 C, temperatures which are only slightly below or above the minimal and maximal growth temperatures. Growing cultures of the thermophile showed little, if any, protein breakdown, a finding similar to that of others with E. coli.


Taxonomic investigations on expressed and cryptic phospho-beta-glucosidases in Enterobacteriaceae. In the Enterobacteriaceae, beta-glucosides are catabolized by a complex system formed of three permeases, with partly overlapping substrate specificities, and two hydrolytic enzymes, phospho-beta-glucosidase A and B, which hydrolyze only phosphorylated beta-glucosides. Some Enterobacteriaceae such as Klebsiella-Aerobacter (Enterobacter) possess the complete system; others possess only parts of it or may have a cryptic phospho-beta-glucosidase activity without permease activity. A screening test applied to strains belonging to several genera of Enterobacteriaceae showed that strains of Citrobacter, Hafnia, and Serratia exhibit a degree of similarity in phospho-beta-glucosidase activity and inducibility which could be useful in their taxonomic characterization; others, such as Aerobacter aerogenes, Erwinia, and Proteus vulgaris, are more heterologous. Owing to the presence of inducible phospho-beta-glucosidases A and B in Citrobacter, the fermentation of beta-methyl glucoside and the fermentation of arbutin in mixture with cellobiose could be of diagnostic value in the differentiation of Citrobacter from Salmonella. Wild-type strains of Escherichia coli, Shigella, and Salmonella are phenotypically similar in their inability to catabolize beta-glucosides, the presence of constitutive P-beta-glucosidase A, and the lack of beta-glucoside permeases I and II. Their beta-glucoside-fermenting mutants show, however, a phospho-beta-glucosidase and beta-glucoside permease activity which is characteristic for mutants from each genus. The differences in the phenotype of the mutants reflect probable differences in the presence of cryptic genes in the wild-type strains and could be of evolutionary significance.


Phospho-beta-glucosidases and beta-glucoside permeases in Streptococcus, Bacillus, and Staphylococcus. The presence of phospho-beta-glucosidases and beta-glucoside permeases was found in strains of Streptococcus, Bacillus, and Staphylococcus. In streptococci, the phospho-beta-glucosidase activity depends on the antigenic group. The highest activity was found in strains of group D. In group D strains, phospho-beta-glucosidase activity is induced by beta-methyl glucoside and cellobiose but not by thiophenyl beta-glucoside (TPG). With the exception of four strains isolated in Japan, all strains of B. subtilis tested possess an inducible phospho-beta-glucosidase activity, beta-methyl glucoside, cellobiose, and TPG acting as inducers. S. aureus strains possess phospho-beta-glucosidase A but not phospho-beta-glucosidase B, whereas most S. albus strains show no detectable phospho-beta-glucosidase activity. The prompt fermentation of beta-methyl glucoside by S. aureus strains could serve as an additional criterion for their differentiation from S. albus. A comparative investigation of the active uptake of (14)C-TPG showed that a Streptococcus group D strain and a B. subtilis strain posses two inducible permeases with characteristics similar to the beta-glucoside permeases I and II of Enterobacteriaceae. In S. aureus, TPG is accumulated by a constitutive permease with high affinity for aromatic beta-glucosides and glucose. The active uptake of TPG by S. aureus appears to depend on the activity of the phosphoenol pyruvate-dependent phosphotransferase system.


Genetic eparation of the inosinic acid cyclohydrolase-transformylase complex of Salmonella typhimurium. Genetic and enzymatic analyses were made with the purH mutants of Salmonella typhimurium. These mutants are purine auxotrophs which are deficient in the conversion of phosphoribosyl-aminoimidazolecarboxamide (AIC) to inosine-5'-monophosphate (IMP). Two steps are required for this process: phosphoribosyl-AIC transformylase (EC 2.1.2.3) and IMP cyclohydrolase (EC 3.5.4.10). Genetic analysis identified two complementation groups, I and II, and a third group of noncomplementing mutants (I-II). Mutations in gene I lead to complete loss of transformylase activity and no loss of cyclohydrolase activity if the mutation is of the missense type, but partial loss if it is of the chain-terminating type (nonsense or frameshift). Gene II mutants are all of the missense type and show normal transformylase activity but no cyclohydrolase activity. The noncomplementing mutants (I-II) are all of the chain-terminating type and are completely deficient in both activities. The results are explained and discussed in terms of subunit interactions of a stable enzyme complex.


Biosynthesis of ubiquinone in Escherichia coli K-12: location of genes affecting the metabolism of 3-octaprenyl-4-hydroxybenzoic acid and 2-octaprenylphenol. Two genes (ubiB and ubiD) concerned with two successive reactions in ubiquinone biosynthesis in Escherichia coli were mapped and found to be closely linked. Mutant strains of E. coli carrying the ubiB(-) and ubiD(-) alleles were shown to accumulate 2-octaprenylphenol and 3-octaprenyl-4-hydroxybenzoic acid, respectively. These compounds were isolated and identified by using nuclear magnetic resonance and mass and infrared spectroscopy. Cell extracts from the mutant strain carrying the ubiD(-) allele lack 3-octaprenyl-4-hydroxybenzoate decarboxylase activity.


R factor transmission in vivo.

R factor transmission in vivo. Experimental infections were induced in weanling pigs orally both with nalidixic acid (NA)-sensitive and -resistant strains of Salmonella choleraesuis var. kunzendorf, designated RC221 and RC221NA, respectively. Prior to the time of infection, cultures of normal bacterial flora were isolated from swine fecal matter and screened for the presence of R factors. A majority of these bacterial isolates harbored transferable resistances. Both strains RC221 and RC221NA have been shown to be competent recipients in vitro of the R factors present in the normal intestinal flora. The property of NA resistance greatly facilitated recovery of the infecting organism. After infection, salmonellae from liver, lung, spleen, lymph node, intestine, and feces were screened for the presence of R factors. Transfer of drug resistance in vivo was a rare occurrence; however, if infected specimens, particularly intestinal, were incubated in nutrient broth prior to plating, R factor transfer occurred, presumably in the test tube. Changes in recipient cultures were frequently observed after introduction of R factors from organisms of pig origin into the S. choleraesuis var. kunzendorf test organisms. Alterations include changes in typing reaction, granular growth in broth, differences in colony form, and reduction of virulence.


Influence of O side chains on the attachment of the Felix O-1 bacteriophage to Salmonella bacteria. The adsorption rate constant (ARC) of the Felix O-1 (FO) bacteriophage to sensitive Salmonella strains was used to determine the effect of variations in surface antigens on phage attachment. The N-acetylglucosamine of the common-core polysaccharide of the Salmonella lipopolysaccharide (LPS) was found to be an essential part of the receptor for the FO phage in conformation with earlier reports. It was found that (i) the ARC was low for strains having O side chains containing two or three non core monosaccharides, (ii) the ARC varied when the O side chain contained no, or only one, noncore monosaccharide, (iii) the ARC was high when the O side chain contained only one repeating unit, and (iv) the ARC was high to mutants of chemotype Ra in which the N-acetylglucosamine was the terminal sugar of the LPS. Since a good correlation was found between the ARC of the FO phage and the phage-inactivating capacity of phenol water-extracted LPS, the results suggest that only the structure and composition of the LPS determines the adsorption rate of the FO phage. The phage-inactivating capacity of LPS from the Ra mutants increased in parallel with higher glucosamine contents in the core polysaccharide. In smooth strains having long and numerous O side chains, the access of the FO phage to its receptor is probably blocked by the presence of the side chains, whereas short and numerous side chains or T1 side chains do not interfere with the FO attachment.


Reversion instability in the galactose operon of Escherichia coli. The gal-3 mutation, which had been shown previously to produce unstable revertants, was combined in three instances with recA, a mutation which suppresses recombination. Unstable revertants were produced in the gal-3 recA recombinants qualitatively as frequently as in the absence of the recA gene, and it is concluded that a recombination mechanism is not the basis of the instability observed.


Thymineless death in Escherichia coli: inactivation and recovery. The effects of chloramphenicol (CAP) on the progress of thymineless death (TLD), nalidixic acid (NA) inactivation, ultraviolet (UV) irradiation, and mitomycin C (MC) inactivation were studied in Escherichia coli B, B(s-1), B(s-3), B(s-12), and B/r. This was done before, during, and after inactivation. During the progress of inactivation, it was found that at 10 to 20 mug of CAP per ml, up to 50% of the UV-sensitive bacteria survived TLD and about 10% survived NA. In E. coli B/r, at these concentrations of CAP, about 10 to 15% of the cells survived TLD and about 20 to 25% survived NA. Concentrations of CAP greater than 25 mug/ml actually increased the sensitivity of E. coli B, B(s-1), B(s-3), and B(s-12) to inactivation by either TLD or NA; at 150 mug of CAP per ml, the sensitivity of E. coli B/r to inactivation also increased. When E. coli B cells were incubated in CAP prior to inactivation, the longer the preincubation the longer onset of TLD was delayed; NA inactivation was also affected in that the rate of inactivation after CAP incubation was greatly decreased. Preincubation of E. coli B/r with CAP had much less effect on the progress of inactivation. After thymineless death, incubation in CAP plus thymine led to a rapid and almost complete recovery of E. coli B and B(s-12). Lesser recoveries were observed after inactivation due to UV, NA, or MC inactivation. E. coli B(s-1) and B/r did not recover viability after any mode of inactivation, and E. coli B(s-3) and B(s-12) recovered from UV to about 20% of the initial titer. It was suggested that protein synthesis, in particular proteins involved in deoxyribonucleic synthesis, was a determining factor in these inactivating and recovery events.


Polyuridylic acid-directed phenylalanine incorporation in minicell extracts. Cell-free extracts of miniature Escherichia coli cells deficient in deoxyribonucleic acid (DNA) and DNA-dependent ribonucleic acid polymerase have been shown to be capable of polyuridylic acid-directed [(14)C]phenylalanine incorporation.


Induced bactericidal response in the hagfish. Hagfish were shown to be capable of synthesizing bactericidins after injection of gram-negative bacteria. The bactericidins could be detected as early as 2 days after injection. The degree of specificity was not as impressive as in mammalian systems.


Auto-immunity and Hashimoto's thyroiditis.


Changes in extracellular space of the mouse cerebral cortex during hydroxyadipaldehyde fixation and osmium tetroxide post-fixation.


A review of the first textbook of pediatric surgery in the English language.


Some properties of isolated neuronal cell fractions.


Thermodynamic treatment of bacteriophage T4B adsorption kinetics. The activation energy was measured for the reaction of inactive phage becoming active in the presence and in the absence of l-tryptophan and was found to be about 50% lower in the former case.


Effect of host cell wall material on the adsorbability of cofactor-requiring T4. The adsorbability of T4 on host cells was determined as a function of time after their liberation from infected cells. Freshly liberated (nascent) particles are readily adsorbed but lose their adsorbability with a half-time of about 2 days at 5 C, but only about 20 min at 37 C. They can be made adsorbable again with an alpha-amino acid cofactor like l-tryptophan, and this state of adsorbability can be stabilized by cell wall material from Escherichia coli. Such stabilized particles lose their adsorbability at a rate similar to that at which nascent particles lose theirs. Most freshly liberated particles are observed by means of electron microscopy to have "debris" attached to their baseplates and to have most of their six, long tail fibers free, whereas "old" particles that have lost their adsorbability appear relatively "clean" with most of their tail fibers wrapped around their sheaths. Nascent particles have densities that are lower than those of old particles. The material responsible for nascent adsorbability seems to be a fragment of the host's cell wall, for nascent adsorbability is destroyed by lysozyme. Furthermore, nascent T4 particles liberated from host cells with radioactively labeled walls carry the label in density gradients but lose it as they lose adsorbability. In addition, only a small proportion of particles liberated from infected spheroplasts are nascently adsorbable, whereas most particles liberated from intact cells are adsorbable.


Obituary: James Michael Anthony O'Donnell.


Kenneth Stokes Smith.

Kenneth Stokes Smith.


Infections due to gram-negative organisms: an analysis of 860 patients with bacteremia at the University of Minnesota Medical Center, 1958-1966.


Complementation between exr and uvr A strains of Escherichia coli.


Therapeutic efficacy of amantadine HCl and rimantadine HCl in naturally occurring influenza A2 respiratory illness in man.


Official directory: international organizations and agencies.


Treatment of strokes. I.

Treatment of strokes. I.


Let's examine: an annotated bibliography on challenge examinations.


Portrait of a leader: Isabel Maitland Stewart.


The history of orthopaedic nurse training.


Localization of the resistance to diffusion in the three-layered placenta of the rabbit.


Hypoglycin and hypoglycin-like compounds.


The application of bacterial genetics to the study of human genetic abnormalities.


Causality, casuistry and clinical carcinogenesis.


Directory of nurses with earned doctoral degrees. Compiled by the American Nurses' Foundation, Inc.


The genetics of intestinal carbohydrate intolerance.


Genetics and the gastrointestinal system.


Review of research on the intelligibility and comprehension of accelerated speech.


Collateral pathways in occlusion of the superior vena cava and its tributaries.


Spectra and analyses of insecticide synergists and related compounds containing the methylenedioxyphenyl group by spectrophotofluorometry (SPF) and spectrophotophosphorimetry (SPP).


Hydrogen phosphide as a fumigant for foods, feeds and processed food products.


On the fungicidal action of phenylmercuric compounds.


Radiotracer studies on metabolism, degradation, and mode of action of insecticide chemicals.


Selective toxicity of systemic insecticides.


Experimental approaches to pesticide photodecomposition.


The in vitro metabolism of organophosphorus insecticides by tissue homoegenates from mammals and insect.


Mechanism of low toxicity of Sumithion toward mammals.


Biochemical genetics of insecticide resistance in the housefly.


The correlation between physiological activity and physicochemical property of the substituted phenols.


Mode of action of agricultural antibiotics developed in Japan.


Louis I. Dublin 1882-1969.

Louis I. Dublin 1882-1969.


Mortimer Spiegelman 1901-1969.

Mortimer Spiegelman 1901-1969.


Memoir of Leighton Francis Appleman. 1874-1968.


Memorial. Arnold Rice Rich. M.D.

Memorial. Arnold Rice Rich. M.D.


Freezing of bacteriophage T4: temperature and rate effects as a function of salt concentration.


Hereditary hyperphosphatasia. Studies of three siblings.


The treatment of chronic biliary salmonella carriers.


Physiology and pharmacology of the coronary circulation and myocardium, particularly in relation to coronary artery disease.


Home health services--past, present, future.


A selected public health bibliography with annotations.


Simultaneous renal allograft nephrectomy and allotransplantation.


Histidine decarboxylase inhibitors and second-set allograft survival.


Urinary tract infection in clinical renal transplantation.


Technical problems in lung allotransplantation. Causes of pulmonary venous outflow obstruction.


Deep vein thrombophlebitis. Current status of etiology and treatment.


The pH-dependence of the binding of competitive inhibitors to pepsin. 1. The pH-dependence of the binding to pepsin of four dipeptide competitive inhibitors is reported. Values of K(i) obtained from equilibrium-dialysis experiments agree closely with those from kinetic measurements. 2. The binding of uncharged N-acyl-dipeptide amides to pepsin is essentially independent of pH from 0.2 to 5.8. Values of K(i) for the corresponding N-acyl-dipeptide acids rise rapidly above pH3.5, and depend on the ionization of a group of apparent pK(a) 3.6. 3. The data indicate that pepsin does not undergo any gross conformation change (at least none that affects binding) over the whole pH range of its catalytic activity. The pH-dependence of the dipeptide acid inhibitors indicates that the acid anions do not bind to pepsin, presumably because of electrostatic repulsion between the inhibitor anion and a negative centre at or near the active site of the enzyme. 4. The binding of all four stereoisomers of N-acetylphenylalanylphenylalanine, of the depside analogues of the l-l- and d-l-compounds and of N-acetylglycyl-l-phenylalanine and N-acetyl-l-phenylalanylglycine was studied at pH2.2. 5. These results throw further light on the binding specificity of pepsin and on the charge nature of the active site of this enzyme.


The pH-dependence of pepsin-catalysed reactions. 1. The pH-dependence of the pepsin-catalysed hydrolysis of three peptide substrates was studied by using a method for the continuous monitoring of the formation of ninhydrin-positive products. 2. Two peptide acid substrates, N-acetyl-l-phenylalanyl-l-phenylalanine and N-acetyl-l-phenylalanyl-l-phenylalanyl-glycine, show apparent pK(a) values of 1.1 and 3.5 in the plots of k(0)/K(m) versus pH. By contrast a neutral substrate, N-acetyl-l-phenylalanyl-l-phenylalanine amide, shows apparent pK(a) values of 1.0 and 4.7. 3. Together with the data of the preceding paper (Knowles, Sharp & Greenwell, 1969), these results are taken to indicate that the rate of pepsin-catalysed hydrolysis is controlled by the ionization of two groups, which on the free enzyme have apparent pK(a) values of 1.0 and 4.7. It is apparent that the anions of peptide acid substrates are not perceptibly bound to the enzyme, resulting in apparent pK(a) values of 3.5 for the dependence of k(0)/K(m) for these materials.


The inhibition of pepsin-catalysed reactions by products and product analogues. Kinetic evidence for ordered release of products. 1. The inhibition of pepsin-catalysed hydrolysis of N-acetyl-l-phenylalanyl-l-phenylalanylglycine by products and product analogues was studied. 2. The non-competitive nature of the inhibition by the product N-acetyl-l-phenylalanine confirms an ordered release of products, and points to a common mechanism (involving an amino-enzyme) for pepsin-catalysed transpeptidation and hydrolysis reactions. 3. N-Acetyl-l-phenylalanine ethyl ester is also a non-competitive inhibitor, but here the inhibition is of the ;dead-end' type. No ethanol is detectable in reaction mixtures, indicating that this ester cannot act as an amino group acceptor in a transpeptidation process. 4. The same is true for N-methanesulphonyl-l-phenylalanine methyl and methyl thiol esters. No methanethiol is liberated when the methyl thiol ester is present as an inhibitor of the hydrolytic reaction, and the hope that such a thiol ester would effectively trap the amino-enzyme was not fulfilled.


The rate-determining step in pepsin-catalysed reactions, and evidence against an acyl-enzyme intermediate. To delineate further the pathway of pepsin-catalysed reactions, three types of experiments were performed: (a) the enzyme-catalysed hydrolysis of a number of di- and tri-peptide substrates was studied with a view to observing the rate-determining breakdown of a common intermediate; (b) the interaction of pepsin with several possible substrates for which ;burst' kinetics might be expected was investigated; (c) attempts were made to trap a possible acyl-enzyme intermediate with [(14)C]methanol in both a hydrolytic reaction (with N-acetyl-l-phenylalanyl-l-phenylalanylglycine) and in a ;virtual' reaction (with N-acetyl-l-phenylalanine) under conditions where extensive hydrolysis or (18)O exchange is known to occur. It is concluded that (i) intermediates in pepsin-catalysed reactions (aside from the Michaelis complex) occur subsequently to the rate-determining transition state, and (ii) an acyl-enzyme intermediate, if such is formed, cannot be trapped with [(14)C]methanol in these systems.


An aspartic acid residue at the active site of pepsin. The isolation and sequence of the heptapeptide. Pepsin reacts stoicheiometrically with the active-site-directed irreversible inhibitor N-diazoacetyl-l-phenylalanine methyl ester, with concomitant loss of all proteolytic and peptidolytic activity. The reagent esterifies a unique aspartic acid residue in pepsin, which is in the sequence:Ile-Val-Asp-Thr-Gly-Thr-Ser


Carabolite repression of the lac operon. Repression of translation. 1. Experiments were devised to show whether catabolite repression of beta-galactosidase synthesis operates at the level of transcription or of translation. Escherichia coli K12 was induced for a short period in non-repressing medium (glycerol-minimal medium), and transcription of the lac operon was terminated by either of two methods; glucose was then added as a source of the catabolite repressor during the subsequent translation of the accumulated beta-galactosidase messenger RNA. 2. When induced bacteria in glycerol medium were infected with T6 phage, which is known to halt transcription, the addition of glucose up to 3min. later diminished the yield of beta-galactosidase. 3. When induced bacteria in glycerol medium were removed from the inducer and resuspended in fresh medium (a process that is also known to halt transcription), the yield of enzyme was again diminished by the presence of glucose in the resuspension medium. 4. It is concluded that repression of beta-galactosidase synthesis can be brought about by the presence of glucose during the translation phase only. 5. In E. coli strain 300U the effect on translation was sufficient to account for almost all the catabolite repression of beta-galactosidase synthesis observed during exponential growth of the organism in glucose-minimal medium. In E. coli strain 200P, however, much more severe repression occurred during exponential growth, and an additional effect of glucose is postulated.


Degradation of human serum albumin by human pepsins.


Metal ion complexes with biotin and biotin derivatives. Participation of sulfur in the orientation of divalent cations.


Structural and functional roles of the cysteine residues in the alpha subunit of the Escherichia coli trypotphan synthetase. II. Functional roles of the cysteine residues.


The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins.


The ribosomal proteins of Escherichia coli. II. Chemical and physical characterization of the 30S ribosomal proteins.


Specificity of the interaction of aminoacyl ribonucleic acid with a protein-guanosine triphosphate complex from wheat embryo.


A simple one-step method for the preparation of highly purified formylmethionine transfer ribonucleic acid and methionine transfer ribonucleic acid from Escherichia coli.


Thymidine triphosphate content of regenerating rat liver.


The isolation and identification of two cytokinins from Escherichia coli transfer ribonucleic acids.


Virus-specific proteins in Escherichia coli infected with phage Qb.


Effect of transfer RNA on the thermal inactivation of rat liver arginyl-RNA synthetase.


Ribosomal protein pools in Escherichia coli.


Incorporation of 5-fluorouracil into the transfer RNA of Escherichia coli K12W6 and its effect on the methylation of uracil.


Canine renal preservation following hypothermic perfusion and subsequent function.


On the ribosomal subparticles formed in Escherichia coli BS-1 irradiated with ultraviolet light: formation and biological function of small particles.


Toleragenic effects of porcine liver allografts.


Liver-cell resting membrane potential as a direct viability assay of preserved organs.


Observations on lactic dehydrogenase and alkaline phosphatase isoenzymes in xenogenic liver grafts.


Heterotropic hepato-enteric block transplantation: a new approach in functional liver replacement.


Mechanisms of leukocyte infiltration of allografts.


Prolonged transplant survival due to non-specific RNA.


Pancreatic allotransplantation in the dog and in man.


Pancreatic islet transplantation.

Pancreatic islet transplantation.


The use of a combined pentagastrin-insulin test to assess the effectiveness of truncal vagotomy.


Human lung transplantation.

Human lung transplantation.


Hyperlipidaemia and pulmonary fat embolism following Escherichia coli bacteriaemia.


Absorption of ethanol from the stomach.

Absorption of ethanol from the stomach.


Gastric and pancreatic secretion in response to gastric distention in dogs.


Percentage absorption of a drug from the stomach.


Escherichia Coli O:14 and colon hemagglutinating antibodies in inflammatory bowel disease.


Research methodology in oral diseases.

Research methodology in oral diseases.


Experiments and reports.

Experiments and reports.


Restoration of enzymic activity by complementation in vitro between mutant alpha subunits of tryptophan synthetase and between mutant subunits and fragments of the alpha subunit.


The formation and properties of dimers of the tryptophan synthetase alpha subunit of Escherichia coli.


Formation and properties of the aminoacyl transfer ribonucleic acid-guanosine triphosphate-protein complex.


Study of the ribosomes of Mycobacterium lepraemurium.


Enzymatic joining of deoxyribonucleic acid strands. 3. Further purification of the deoxyribonucleic acid ligase from Escherichia coli and multiple forms of the purified enzyme.


Purification and characterization of chicken pepsinogen and chicken pepsin.


Chemotherapeutic trials in leprosy. 7. Trial of 50 mgm. DDS twice weekly in the treatment of lepromatous leprosy.


Influence of pituitary stalk section on growth hormone, insulin and TSH secretion in women with metastatc breast cancer.


Effect of the C-terminal tetrapeptide amide of gastrin on insulin secretion in man.


Artifactual elevation of PBI from an iodinated dye used to stain medicinal capsules pink.


Maintenance of infectivity of M. lepraemurium in vitro for sixteen years by means of lyophilization. Determination by bacteriologic and morphologic observation of the infecting bacilli.


Breakdown of DNA during growth of Escherichia coli 15T-in ethionine: lack of correlation with strand polarity.


The role of aggressive surgical treatment in the severely burned patient.


Correlation of histopathologic changes in the liver and bone marrow of leprosy patients.


Experimental coliform mastitis in rabbits. A biochemical and histopathological study.


Glomerular basement membrane: insights from molecular models.


Stress-related gastrointestinal dysfunction and combined antidepressant-tranquilizer therapy.


A study of the long-term effect of therapy in chronic obstructive pulmonary disease.


Chronic nonhemolytic unconjugated hyperbilirubinemia with glucuronyl transferase deficiency. Clinical, biochemical, pharmacologic and genetic evidence for heterogeneity.


Coexistence of systemic lupus erythematosus and rheumatoid arthritis. Report of a case and review of the literature, with clinical, pathologic and serologic observations.


Kidney transplantation. A comparison of results using cadaver and related living donors.


The outcome of urinary tract infections in patients after human cadaveric renal transplantation.


The results of urinary diversion using the colonic conduit.


Intravesical pressure measurements: simultaneous filling and recording through one suprapubic cannula.


Child development.

Child development.


An attempt to measure psychological distance in advantaged and disadvantaged children.


Adenosine triphosphate levels of mammalian pancreatic B cells after stimulation with glucose and hypoglycemic sulfonylureas.


Potentiation of the plasma insulin response to glucose by prior administration of alcohol. An apparent islet-priming effect.


Dr. James Marcus Taylor: member first Mississippi State Board of Health.


Mass spectral discrimination between monoenoic and cyclopropanoid, and between normal, iso, and anteiso fatty acid methyl esters. Measurement of the ratio of the intensity of the parent molecular ion to that of the parent molecular ion minus 32 (the mass of methanol) can distinguish between monoenoic and cyclopropanoid fatty acid methyl esters with the same carbon number. A similar technique can be used to distinguish between isomeric normal, iso, and anteiso fatty acid methyl esters.


Bacteriology of earphone contamination.

Bacteriology of earphone contamination.


Clinical evaluation of renafur (NF-246): final report.


Bronchial lavage and intermittent positive-pressure ventilation in the treatment of persistent bronchospasm.


Indicators of homosexuality in the human figure drawings of heroin- and pill-using addicts.


The psychology of memory--1967: a bibliography.


The Z-blepharoplasty.

The Z-blepharoplasty.


Steinmann pin fixation of extremities for cross-leg flaps.


Letter to the editor. Notes on the Chinese Medical Journal and Ronald Y. Sung, D.D.S., M.D.


Victor Tausk's contribution to psychoanalysis.


Cognitive dissonance: a bibliography of its first decade.


Prof. Dr. Janina Hurynowicz (1894-1967).


Identification of Haemophilus vaginalis.


Timely topics in general microbiology, 1967-68.


Timely topics in microbiology: enterics 1967-1968.


Deoxyribonucleic acid-polylysine complexes. Structure and nucleotide specificity.


The mechanistic significance of phosphate labeling of alkaline phosphatase.


Robin Fåhraeus and the discovery of the erythrocyte sedimentation test.


The binding of small cations to deoxyribonucleic acid. Nucleotide specificity.


A method for locating 4-thiouridylate in the primary structure of transfer ribonucleic acids.


Structural studies on transfer ribonucleic acid. I. Labeling of exposed guanine sites in yeast phenylalanine transfer ribonucleic acid with kethoxal.


Aminoacyl nucleosides. VI. Isolation and preliminary characterization of threonyladenine derivatives from transfer ribonucleic acid.


Aminoacyl nucleosides. VII. N-(Purin-6-ylcarbamoyl)threonine. A new component of transfer ribonucleic acid.


Kinetics of synthesis and utilization of adenosine triphosphate by intact cells of Rhodospirillum rubrum.


Dental research at the National Bureau of Standards 1919-1969. History of Dental Research Section.


Use of the cephalosporins in the identification of Klebsiella.


"Aggression" -- biophysiological aspects.


Control of sodium reabsorption.

Control of sodium reabsorption.


Gustav J. Martin.

Gustav J. Martin.


Supplementary proteases and gastric digestion.


Attempt at inducing male-specific antigen in female skin.


CHA personal members.

CHA personal members.


Tagging of small animals with radioisotopes for tracking purposes: a literature review.


Prospects and possibilities in the development of behaviour therapy.


Emoonal indicators  human figure drawings of boys and girls from lower and middle-class backgrounds.


Controlled trials in behaviour therapy.

Controlled trials in behaviour therapy.


Arthrogryposis with meningomyelocele, polythelia and absent ribs.


Kidney transplantation in Iowa.

Kidney transplantation in Iowa.


Iowa physicians want medicaid to operate efficiently and economically.


Medical practice--the government's role in its future.


Early history of medical education in Nashville.


Clinical effects of hypnotics. I. A controlled trial.


A bullet and a bull moose.

A bullet and a bull moose.


Tertiary hyperparathyroidism after renal transplantation.


Effect of cyproheptadine on appetite and weight gain in adults.


A review of the literature on burns and trauma September 1966 to August 1967.


Historical aspects of the role and function of nursing services in the Department of National Health and Welfare.


Structure of transfer RNA. Evidence for interaction between two non-adjacent nucleotide residues in tRNA from Escherichia coli.


Recessive lethal nonsense suppressor in Escherichia coli which inserts glutamine.


Iron-sulphur proteins.

Iron-sulphur proteins.


70S ribosomes are active.

70S ribosomes are active.


Transfer of immunity against long surviving rat skin xenografts by antilymphocyte serum treated Syrian hamsters.


Innervation of the transplanted heart.

Innervation of the transplanted heart.


Hemodynamic observations after cardiac transplantation.


Use and effects of Mace.

Use and effects of Mace.


Dentistry's responsibility to the cleft palate patient.


A swinging approach.

A swinging approach.


Fractures of the femoral shaft in children.


A technique for studying biological reaction rates at high pressure.


Surface areas of the cerebral cortex of mammals determined by stereological methods. The surface areas of the cerebral cortex excluding archipallium of 20 human, 11 cetacean, 6 carnivore, and 5 marsupial brains were determined by stereological methods. There exist rather strict relationships between volume, length of superficially exposed gyri, and cortical surface area.


Virus of the 1918 influenza pandemic era: new evidence about its antigenic character. In serums of unusually isolated Pacific islanders whose only exposure to influenza occurred during the era of the 1918 pandemic the residual neutralizing antibody was greatest to the PR/8 and BH strains of human type A influenza virus, significantly lower to swine influenza virus, and absent to equine or later human type A virus strains. The pandemic virus was thus antigenically closer to human type A strains isolated during the middle 1930's than to other known influenza virus types.


Activity of pyrantel against Parascaris equorum.


Rabies in wild animals trapped for pelts.


Experimental ostertagiasis in lambs fed high and low protein (Nx6.25) grassmeal.


Progress in kidney transplantation.

Progress in kidney transplantation.


Acromegaly: treatment by cryoablation.

Acromegaly: treatment by cryoablation.


The teratogenicity of barbital sodium in mice.


Clinical neurophysiology. Newer diagnostic and therapeutic methods in neurological disease and behavior disorders.


Study of translocation in polypeptide synthesis.


Carbon dioxide regulation of formate hydrogenlyase in Escherichia coli.


Studies on the formation of transfer ribonucleic acid-ribosome complexes. X. Phenylalanyl-oligonucleotide binding to ribosomes and the mechanism of chloramphenicol action.


Peptide bond synthesis in the fragment reaction catalyzed by mammalian liver ribosomes.


The appearance of phage associated lysozyme in E. coli B cells immediately after infection with phage T2.


Genetic and biochemical studies on cell wall peptidoglycan synthesis in Escherichia coli K-12.


The alpha-glucosidases of Streptococcus pyogenes and derived L form.


Blister fluid in pemphigus.

Blister fluid in pemphigus.


Effects of methotrexate upon the experimental Mitsuda reaction.


History's lessons.

History's lessons.


Malignant cultural deprivation--its evolution.


The human biochemical genetics of amino acid transport.


The effect of alcohol on sensory phenomena and cognitive and motor tasks.


Post-weaning enteritis and dysentery.

Post-weaning enteritis and dysentery.


Distribution of efferent nerve endings in the organ of Corti. Their graphic reconstruction in cochleae by localization of acetylcholinesterase activity.


A substate-induced conformation change in the reaction of alkaline phosphatase from Escherichia coli. 1. Benzyl phosphonates were prepared and their potentialities as chromophoric reagents for the exploration of the substrate-binding site of Escherichia coli alkaline phosphatase were investigated. 4-Nitrobenzylphosphonate is a competitive inhibitor of the enzyme. 2-Hydroxy-5-nitrobenzylphosphonate changes its spectrum on binding to the enzyme. This spectral change is reversed when the phosphonate is displaced from the enzyme by substrate. 2. The kinetics of the reaction of 2-hydroxy-5-nitrophenylphosphonate were studied by the stopped-flow and the temperature-jump techniques. It was found that the combination of the phosphonate with the enzyme occurred in two successive and reversible steps: enzyme-phosphonate complex-formation followed by rearrangement of the complex. The spectral change is associated with the rearrangement. At pH8 in 1m-sodium chloride at 22 degrees the rate constant is 167sec.(-1) for the rearrangement of the initially formed binary complex and is 18sec.(-1) for the reverse process. 3. It has previously been proposed that the reactions of phosphatase with its substrates include a distinct step between enzyme-substrate combination and chemical catalysis. The rate constant involved could be predicted but not measured from experiments with substrates. The value for the rate constant measured from the rate of the enzyme-phosphonate rearrangement is in excellent agreement with the predicted value. A model for the reaction mechanism is proposed that includes a conformation change in response to phosphate ester binding before phosphate transfer from substrate to enzyme.


Primary sequences and their determination.


The effect on transfer ribonucleic acid of moification in vitro.


A comparative study of the effect of aflatoxin B and actinomycin D on HeLa cells. 1. The cytotoxic effects of aflatoxin B(1) on HeLa cells were examined and effects of short exposures of the cells to the toxin were found to be reversible. 2. Aflatoxin B(1) inhibited the synthesis of both ribosomal and heterodisperse RNA. It is proposed that the toxin's mechanism of action on ribosomal RNA synthesis is related to its inhibitory effect on the maturation of the 45s-ribosomal-RNA precursor. 3. Protein synthesis is inhibited to a greater extent by aflatoxin B(1) than by actinomycin D. In contrast with actinomycin D, aflatoxin B(1) was shown to disaggregate polyribosomes directly.


Catabolite repression of the lac operon. The contribution f trascriptional repression. 1. Experiments were carried out to distinguish the contributions of transcriptional and translational repression to catabolite repression of the lac operon. 2. In strain EZ16-3-G of Escherichia coli the synthesis of thiogalactoside transacetylase is directed by a gene situated on an episome, and the operator, promotor and regulator genes that lay cis to this gene have been deleted, so that the normal mechanism for controlling transcription is abolished. The extent of catabolite repression in this strain was much less than that in wild-type strains. 3. The same episome is responsible for the synthesis of thiogalactoside transacetylase in strain RM32/F'd25, and in this strain a second lac operon directs the synthesis of beta-galactosidase under the control of a wild-type operator-promotor-regulator system. The extent of catabolite repression of thiogalactoside transacetylase in strain RM32/F'd25 was substantially more than in strain EZ16-3-G, but less than that of beta-galactosidase in strain RM32/F'd25. 4. Since the synthesis of thiogalactoside transacetylase in these organisms is presumably subject to translational repression only, it is concluded that in strain RM32/F'd25 the synthesis of beta-galactosidase is subject to both transcriptional and translational repression. It is also concluded that the extent of translational repression varies between strains.


Catabolite repression of the lac operon. Separt epressionof two enzymes. 1. Catabolite repression of beta-galactosidase and of thiogalactoside transacetylase was studied in several strains of Escherichia coli K 12, in an attempt to show whether a single site within the structural genes of the lac operon co-ordinately controls translational repression for the two enzymes. In all experiments the rate of synthesis of the enzymes was compared in glycerol-minimal medium and in glucose-minimal medium. 2. In a wild-type strain, glucose repressed the synthesis of the two enzymes equally. 3. The possibility that repression was co-ordinate was investigated by studies of mutant strains that carry deletions in the genes for beta-galactosidase or galactoside permease or both. In all of the strains with deletions, the repression of thiogalactoside transacetylase persisted, and it is concluded that there is no part of the structural gene for beta-galactosidase that is essential for catabolite repression of thiogalactoside transacetylase. 4. Subculture of one strain through several transfers in rich medium greatly increased its susceptibility to catabolite repression by glucose. It is concluded that unknown features of the genotype can markedly affect sensitivity to catabolite repression. 5. These results make it clear that one cannot draw valid conclusions about the effect of known genotypic differences on catabolite repression from a comparison of two separate strains; to study the effect of a particular genetic change in a lac operon it is necessary to construct a partially diploid strain so that catabolite repression suffered by one lac operon can be compared with that suffered by another. 6. Four such partial diploids were constructed. In all of them catabolite repression of beta-galactosidase synthesized by one operon was equal in extent to catabolite repression of thiogalactoside transacetylase synthesized by the other. 7. Taken together, these results suggest that catabolite repression of beta-galactosidase and thiogalactoside transacetylase is separate but equal.


The tertiary structure of transfer ribonucleic acid investigated by a chemical modification technique.


Effects of limiting different urine nucleotide precursors on nucleic acid synthesis and nucleotide pools of purine-requiring mutants of Escherichia coli.


Compartmentation of terpenoid biosynthesis in green plants. A proposed route of acetyl-coenzyme A synthesis in maize chloroplasts. On the basis of radioisotope-incorporation experiments it is suggested that acetyl-CoA, an obligatory intermediate in chloroplast terpenoid biosynthesis, may be formed in maize from photosynthetically fixed carbon dioxide by the route carbon dioxide-->glycollate-->glyoxylate-->glycine-->serine-->pyruvate-->acetyl-CoA. The proposed route is supported by conventional radioisotope-dilution studies and by experiments with inhibitors affecting reactions involved in the pathway. The proposed route appears to play little part in formation of extrachloroplastidic sterol.


Effect of hypermethylation on the functional properties of transfer ribonucleic acid. 1. The ability of chemically hypermethylated Escherichia coli B transfer RNA to accept 19 amino acids was studied and the results were compared with those obtained with a control sample of E. coli B transfer RNA incubated under similar conditions in the absence of methylating agent. 2. There is a marked decrease in the ability of the modified transfer RNA to accept amino acids in almost all instances. 3. The acceptance of cysteine appears to be unique in that it is enhanced in the hypermethylated transfer RNA. 4. More detailed studies on the kinetics of acceptance for six amino acids is presented, emphasizing the variation in response of the individual amino acids. 5. Increasing hypermethylation causes a progressive decrease in the amino acid acceptance. 6. The results are discussed in terms of methylation at functional sites within the transfer RNA and possible conformational alterations to the structure of the macromolecule.


A simple method for simultaneous determination of plasma and red cell volume.


Clinical studies on induction agents. 28. A further comparison of venous complications following thiopentone, methohexitone and propanidid.


Splitting of human thyroglobulin. II. Enzymatic digestion.


The immunopathology of pemphigus and bullous pemphigoid.


Telescope crown retainers for removable partial dentures.


Recent knowledge of the dental pulp and its application to clinical practice.


Origins of Oesophagology.

Origins of Oesophagology.


Clinical trials of analgesic drugs.

Clinical trials of analgesic drugs.


Review of thyroid carcinomas in the United Cardiff Hospitals.


Anterior cervical disc removal and fusion.


The long term results of early closure of myelomeningoceles.


Lung transplantation.

Lung transplantation.


Differentiation of organic from nonorganic retarded children.


Achievement motivation among Negro adolescents in regular and special education programs.


Use of punishment procedures with the severely retarded: a review.


Bone marrow auto-transplantation in animals after administration of high dosage of cytotoxic drugs.


Folic acid deficiency in pregnancy. On the recognition, pathogenesis, consequences, and therapy of the deficiency state in human reproduction.


A study on the dissociation of Escherichia coli ribosomes.


Physical fitness and emotional health: a review.


Kinetic mechanism of maltodextrin phosphorylase.


Use of radioactive media in assessment of renal function: a review.


Symposium on criteria for tooth removal. Foreword.


Prosthodontic considerations in tooth removal.


The overdenture.

The overdenture.


Esthetic considerations prior to tooth removal.


Medication attitudes and drug response.

Medication attitudes and drug response.


A technical consideration in child analysis and child therapy.


Affective disorders and norepinephrine pharmacology.


Twinship and psychological differentiation.


Some aspects of Charcot's influence on Freud.


A cyclone separator for aerosol sampling in the field.


Effect of different freeze-thaw regimes on ice formation and ultrastructural changes in tomato fruit parenchyma tissue.


The source of bacteria in fresh cream, and the methylene blue reduction test as a guide to hygienic quality.


Strontium chloride and strontium selenite enrichment broth media in the isolation of Salmonella.


The effect of several rates of freezing and thawing on the viability of Mycobacterium leprae.


Antibody response and reactions to aqueous influenza vaccine, simple emulsion vaccine and multiple emulsion vaccine. A report to the Medical Research Council Committee on influenza and other respiratory virus vaccines.


Terminal bronchopneumonia. A bacteriological and histological study of 111 necropsies.


Salmonellas in sewage. A study in latent human infection.


Shigella sonnei mutants rapidly fermenting lactose. I. Formation and properties.


Isolation of an Escherichia coli serotype provoking keratoconjunctivitis in guinea pigs.


Fimbrial antigens of Sh. flexneri bacterie and the methods of their serological study. I.


Isolation of subserotypes of leptospirae of the Tarassovi S. Hyos group, previously unknown in the U.S.S.R.


HTP and DCT indicators of sexual identification in children.


Surgical and photographic aspects of microvascular surgery.


John Addenbrooke and his hospital.

John Addenbrooke and his hospital.


Automatic theatre cameras: a review.

Automatic theatre cameras: a review.


When do patients need corneal transplants?


Control of template specificity of E. coli RNA polymerase by a phage-coded protein.


Elias Sabot and King Henry IV.

Elias Sabot and King Henry IV.


Nitrogen retention in man in relation to the level and pattern of essential amino acids.


Psychoanalytic remarks on a poem by Emily Dickinson.


Psychoanalysis and American psychiatry.

Psychoanalysis and American psychiatry.


Pacific Coast Obstetrical and Gynecological Society: directory.


Changes in height of the alveolar process in edentulous segments. II. A longitudinal clinical and radiographic study over 5 years of full upper denture patients with residual lower anteriors.


Grant S. Beardsley, 1878-1968.

Grant S. Beardsley, 1878-1968.


Bernard J. Hanley, 1897-1967.

Bernard J. Hanley, 1897-1967.


The growth-duplication cycle. IX. Control of growth and cell division. ANL-7535.


A kinetic model of photoreactivation in UV-irradiated bacteria. ANL-7535.


Photomutagenesis in chemostat cultures of bacteria. ANL-7535.


Clinical evaluation of doxepin and diazepam in patients with gastrointestinal disease and anxiety. A controlled double blind study and long-term evaluation.


Genetical and physiological studies of a large-cell radiation-resistant strain of Escherichia coli. I. Genetic analysis. ANL-7535.


Genetical and physiological studies of a large-cell radiation-resistant strain of Escherichia coli. II. Physiological studies. ANL-7535.


Effects of cell size and DNA synthesis on radiation sensitivity of Escherichia coli. ANL-7535.


A method for the study of cell proliferation of the hair follicle. ANL-7535.


The growth-duplication cycle. 8. A simple model for distribution of cell volume based on linear growth. ANL-7535.


Growth characteristics of type E Clostridium botulinum in the temperature range 34 degrees F to 50 degrees F. COO-1183-30.


Russian radioecology. A bibliography of Soviet publications with citations of English translations and abstracts. TID-3915.


Laboratory testing of silver amalgarestorative material with stannous fluoride. SAM-TR-69-9.


A clinical comparison of four posterior intermediate restorative materials. SAM-TR-68-124.


Implantation of multilead electrode assemblies and radio stimulation of the brain in chimpanzees. ARL-TR-69-2.


Mammalian adaptations to diving. Rep No 562.


Studies on the avian gizzard: Auerbach's plexus.


An electron microscopic study of the human infundibulum.


The fine structure of the terminal sensilla on the maxillary palps of Schistocerca gregaria (Forskål) (Orthoptera, Acrididae).


On the nature of the action of polyamines on Escherichia coli conjugation.


Scanning electron microscopy of primary membrane bone.


Effect of phenethyl alcohol on induced L-tryptophanase synthesis in Escherichia coli.


The genophore replication during successive cell separation cycles in Escherichia coli K-12 synchronized with the stationary-phase method.


The mechanism of conjugation and recombination in bacteria. VII. Further characteristic of recombination-deficient Hfr mutants.


Mechanism of conjugation and recombination in bacteria. VI. Receptibility of F- cells for donor genophore during the cell-life cycle.


The mechanism of conjugation and recombination in bacteria. 8. Amino acid deprivation in Hfr and-or F- cells; effect on recombination.


Pattern of genophore replication in Hfr and F- cells of Escherichia coli K-12.


Effects of medium-chain triglycerides on gastric secretion in rats and dogs.


Clinical investigation: a guide to its evaluation.


Studies on trachoma. VI. Microbiological observations in a field trial in Saudi Arabia of bivalent rachoma vaccine at three dosage levels.


Preservation of alveolar cell metabolism by successful immune suppression after canine lung transplantation.


Current concepts of peptic ulceration.

Current concepts of peptic ulceration.


Electrocardiographic findings in cardiac transplantation.


Immunofluorescence staining properties of different conjugates.


Hemodynamic and body composition changesfollowing bilateral nephrectomy in chronic renal failure.


Yamagiwa's tar cancer and its historical significance--from Yamagiwa to Kennaway.


Metochlopramide as an adjunct to small bowel intubation.


Effect of metoclopramide on gastric function in man. The effects of metoclopramide on gastric emptying and gastric secretion have been assessed in man using the double sampling test meal. Metoclopramide increases the rate of emptying of the stomach. The magnitude of the effect is directly related to the initial emptying time. Metoclopramide has no effect on the acid response to a water test meal.


Combined effects of ampicillin and methiciillin on cell wall morphology of a methicillin-resistant Staphylococcus.


Hamartomas in the alimentary tract.

Hamartomas in the alimentary tract.


The use of thin-layer chromatography in following product formation in the photolysis of alpha-lipoic acid.


Human cardiac transplantation: clinical experience.


Function of the transplanted human heart.


Hypothalamic tonic regulation of blood pressure in spontaneously hypertensive rats.


Factors influencing the formation of immunofluorescent antigen in a Burkitt lymphoma cell line.


Effect of Escherichia coli and its endotoxin on the resistance of mice to experimental cryptococcal infection.


Quantitative study of glomeruli. Focal glomerulonephritis and diabetic glomerulosclerosis.


Medical history museum.

Medical history museum.


Integrated EEG. variability: drug effects in acute schizophrenics.


The right of the insane.

The right of the insane.


The curious interrelation between cancer and diseases of the arteries.


A compendium of nutritional status studies and dietary evaluation studies conducted in the United States, 1957-1967.


Effect of solvent on the photolysis of alpha-lipoic acid.


The MRI story.

The MRI story.


Experiment versus authority.

Experiment versus authority.


Animal models--a neglected medical resource.


Peritoneal dialysis.

Peritoneal dialysis.


Diagnostic concepts concerning patients demonstrating both psychotic and neurotic symptoms.


Psychiatry and objectivity. Method in the therapeutic use of movement, music and creativity.


In memoriam F.C.D.

In memoriam F.C.D.


Rejection of the cardiac transplant.

Rejection of the cardiac transplant.


Serotyping for homotransplantation. XXIV. Heart transplantation.


Antigenic composition of heart tissue.

Antigenic composition of heart tissue.


Bougon on coronary aneurysm (1812).

Bougon on coronary aneurysm (1812).


Heart transplantation in man: compilation of cases. II. January 23, 1964 to June 22, 1969.


Primary lymphomas of the small intestine including the duodenum. A roentgen analysis of twenty-nine cases.


The esophagogastric junction.

The esophagogastric junction.


E. Walter Hall, M.D., 1893-1969.

E. Walter Hall, M.D., 1893-1969.


A survey of progress and problems in surgery of the heart.


Some controversial topics in vascular surgery.


Abdominoperineal resection of the rectum. Preliminary experience with a simplified technic.


The place of electrosurgery in abdominal operations.


Rhabdomyosarcoma.

Rhabdomyosarcoma.


A late complication of inversion of the appendiceal stump.


Simplified method for repair osliding inguinal hernia.


Differential inhibitor sensitivities of thymidine phosphorylases from E. coli and mammalian tissues.


The basic equation for the coagulation of milk and blood compared with the Maxwell (relaxation) and Kelvin (retardation) equations.


Specific drugs against influenza.

Specific drugs against influenza.


Bacteriological swabs.

Bacteriological swabs. Streptococcus pyogenes survives poorly on plain cotton-wool swabs, whereas serum-dipped swabs permit its survival but also allow overgrouth by other bacteria and are likely to contain virus inhibitors. The use of swabs dipped in a solution of 20% bovine albumin (fraction V) and sterilized by steam is therefore recommended, since these permit bacterial survival without growth and are unlikely to contain virus inhibitors.


We salute M. Digby Leigh, M.D.

We salute M. Digby Leigh, M.D.


Renal allograft with massive perirenal accumulation of lymph.


Control of sodium reabsorption.

Control of sodium reabsorption.


Intermittent positive-pressure ventilation in chicken-pox pneumonitis.


Birthplace of Thomas Willis.

Birthplace of Thomas Willis.


The other side of the brain. II. An appositional mind.


The distribution between the intravascular and interstitial compartments of commonly utilized replacement fluids.


Experience in anesthesia for liver transplantati.


Anesthetic management of tracheal resection and reconstruction.


On collecting drug jars.

On collecting drug jars.


Clinical significance of urinary LDH, alkaline phosphatase and other enzymes.


The surgical treatment of vertebral-basilar aneurysms.


Intracranial anurysmal formations.

Intracranial anurysmal formations.


Neurosurgery in the control of unmanageable affective reactions: a critical review.


The use of neuroleptic agents in a neurosurgical unit.


Congress of Neurological Surgeons, Inc.

Congress of Neurological Surgeons, Inc.


Microelectrode recording during stereotactic surgery.


Selection, timing, and technique of aneurysm surgery of the anterior circle of Willis.


Norman M. Dott: a biographical sketch.

Norman M. Dott: a biographical sketch.


Medicare, medicaid reforms urged by advisory committee.


Stereochemistry of chorismic acid biosynthesis.


Steric course of the chorismate synthetase reaction and the 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthetase reaction.


The occurrence of 4-methylthiazole-5-acetic acid as a thiamine metabolite in rabbit, dog, man and rat.


Reversibility and stability of Escherichia coli ribosomes dissociated by ultraviolet light.


Evidence of hydrogenation of cis-2 double boning on a way of beta-oxidation by Escherichia coli.


Korthof's solid medium and the role of specific and nonspecific inhibitors in the cultivation of leptospirae in tissues.


Use of a micromodification of indirect haemolysis and indirect haemagglutination in the study of antibody synthesis by individual cells of immune animals.


Control of clotting factor biosynthesis by vitamin K.


Transfer of tetracycline resistance factor in field conditions.


Professor Pavel Macúch quinquagenarian.

Professor Pavel Macúch quinquagenarian.


Metabolism and function of vitamin E.

Metabolism and function of vitamin E.


Professor Lev Alexandrovich Zilber, 20-3-1894--10-11-1966.


Recent developments in the fat-soluble vitamins. Metabolism and function of vitamin A.


Substrate requirements for initiation and chain growth of enzymically synthesized RNA.


Substrate specificity of ribosomal peptidyl transferase: 2'(3')-O-aminoacyl nucleosides as acceptors of the peptide chain on the amino acid site.


The UGA codon in vitro: chain termination and suppression.


Role of RNA in the assembly process of bacteriophage fr.


Cell division and DNA replication following a shift to a richer medium.


A mechanism for repressor action.

A mechanism for repressor action.


Genetic evidence that the operator locus is distinct from the the z gene in the lac operon of Escherichia coli.


Biochemical evidence that the operator locus is distinct from the z gene in the lac operon of Escherichia coli.


Variation of the average rotation angle of the DNA helix and the superhelical turns of covalently closed cyclic lambda DNA.


Intragenic initiations of transcription of the tryptophan operon in Escherichia coli following dinitrophenol treatment without tryptophan.


Heterogeneous initiation and termination of enzymically synthesized ribonucleic acid.


Fate of lambda DNA in a bacterial host defective in DNA synthesis.


Directed transposition of the arabinose operon: a technique for the isolation of specialized transducing bacteriophages for any Escherichia coli gene.


Functions of two genes in the first-step-transfer DNA of bacteriophage T5.


Studies on valyl-tRNA synthetase and tRNA from Escherichia coli. II. Interaction between valyl-tRNA synthetase and valine acceptor tRNA.


Amber mutants of the trpR regulatory gene.


Studies on valyl-tRNA synthetase and tRNA from Escherichia coli. I. Purification and properties of the enzyme from normal Escherichia coli strains.


Lipids containing trans-unsaturated fatty acids change the temperature characteristic of thiomethylgalactoside accumulation in Escherichia coli.


Studies on valyl-tRNA synthetase and tRNA from Escherichia coli. 3. Valyl-tRNA synthetases from thermosensitive mutants of Escherichia coli.


Resonance energy transfer in pepsin conjugates.


X-ray scattering study of ribosomes from Escherichia coli.


Kubie's dream and its impact upon reality. Psychotherapy as an autonomous profession.


Donald Darrow Matson: November 28, 1913--May 10, 1969.


Harvey Cushing. General surgeon, biologist, professor.


The development of a mechanical analog for directing and tracking the electrode during stereotaxic operations. Technical note.


Peter Mark Roget (1779-1869). Roget's Thesaurus.


Evaluation of long-term use of propranolol in angina pectoris.


Language and speech correlates of anatomically verified lesions in thalamic surgery for parkinsonism.


The MRI story.

The MRI story.


Medicine in Singapore.

Medicine in Singapore.


Let us now praise five famous men.

Let us now praise five famous men.


E. coli sigma factor: a positive control element in phage T4 development.


Isolation and characterization of streptolydigin resistant RNA polymerase.


Functional independence of F and I sex pili.


Elie Metchnikoff, Ph. D. (1845-1916). Professor at Pasteur Institute.


Action of the associated application of epsilon-acetamido-caproic acid and Trasylol on the survival of skin allografts in the rat.


The London Obstetrical Society.

The London Obstetrical Society.


John Squire and the development of experimental pathology in Birmingham.


Hippocrates and proctology.

Hippocrates and proctology.


Immunofluorescence and tissue culture.

Immunofluorescence and tissue culture.


Immunosuppression and its complications.


A comparative study of the insulin content of islet tissue of various species using anti-insulin serum and the epididymal adipose tissue methods.


Detection of microbial antibodies in chickens: immunofluorescence studies with serum and egg yolk.


Tropical neurology.

Tropical neurology.


Comparative pyrogenic reactivity of rabbit and man to bacterial endotoxin.


Effect of hypophysectomy on immunological recovery after sublethal irradiation of adult rats.


Intra-abdominal homologous heart transplantation.


Demonstration of cytotoxic humoral substances during the course of first set skin allograft rejection.


Experimental infections of lambs with Trichostrongylus axei; investigations using abomasal cannulae.


Keratoplasty in the dog.

Keratoplasty in the dog.


The search for insulin.

The search for insulin.


President of the B.V.A. 1969-70, Mr. John Henry Parsons, M. R. C.V.S.


The clinical evaluation of dxepin--a new psychotherapeutic agent.


Membership list of Cincinnati Dental Society as of September 1, 1969.


The membership of the Omaha District Dental Society 1969.


The differential prediction of response to two anti-psychotic drugs.


Treatment of depression following surgical removal of a rejected renal homotransplant.


The pin retained fixed bridge.

The pin retained fixed bridge.


Haloperidol in the treatment of acute alcoholism.


The use of preformed wax and plastic patterns for crown and bridge castings in metal or of metal bonded with porcelain or plastic.


Chicago Dental Society Branch Roster of Membership 1969-1970.


Changing attitudes of the Veterans Administration and its interaction with the Harvard School of Dental Medicine.


1969-70 membership directory.

1969-70 membership directory.


Membership Directory--1969, California Dental Association, August 1, 1969.


Mercer Dental Society 1969-1970 membership list.


Georgia LPN undergoes kidney transplant.


Roster of Mississippi dentists.

Roster of Mississippi dentists.


The one appointment mandibular plaster impression.


More retentive complete dentures.

More retentive complete dentures.


American Academy of Implant Dentistry directory of membership.


Staff and stations of the Division of Dental Health as of 1 April 1969.


Localization of hematopoietic marrow in jaws of primates.


Iatrogenic ergotism. Report of a case.

Iatrogenic ergotism. Report of a case.


Basic books for the operating room. Supplement V.


Principal helminthozoonoses of the population of the northern districts of the USSR.


Intracellular avian infectious bronchitis virus: detection by fluorescent antibody techniques in nonavian kidney cell cultures.


The biochemical lesion and its historical development.


Synthesis and microbiological properties of dipeptides containing cyclopentaneglycine and beta-2-thienylalanine.


Kinetics and mechanisms of action of drugs on microorganisms. 8. Quantification and prediction of the biological activities of meta- and para-substituted N1-phenylsulfanilamides by microbial kinetics.


Charge-spatial models: cis- and trans-3- and -4-Substituted cyclohexyl phosphates as analogs of 2'-deoxyuridine 5'-phosphate.


Amides of N-acylcysteines as potential amino acid antagonists in bacteria.


The preparation and antimycotic properties of derivatives of 1-phenethylimidazole.


The derivatives of a chloramphenicol-resistant R factor.


Cancer of the urogenital tract: prostatic cancer. Comment: the penultimate in cancer diagnosis--enzymology.


Plants used against cancer. A survey.

Plants used against cancer. A survey.


Intermittent positive-pressure ventilation following pectoplasty.


Effect of alpha-chloroacetophenone on norepinephrine metabolism in rabbit brain in vitro.


Isolation of adenyl cyclase from Escherichia coli. We have found a soluble cyclase, using for assay radioactively marked ATP as precursor. The reaction product was isolated by thin-layer chromatography and identified by specific degradation. After homogenization, part of the activity remained in the particulate fraction but could be easily extracted. The cyclase was concentrated 100-fold by conventional methods. The enzyme has a Mg(++) requirement and is inhibited by fluoride and inorganic pyrophosphate.


The effects of SKF 525-A on growth and amino uptake by E. coli (diploid mutant).


Visit to an organ-transplant unit. 2. The surgery.


Sensitivity of four finger sweat print durations to an anticholinergic agent.


Edwin Butterworth Mains, 1890-1968.

Edwin Butterworth Mains, 1890-1968.


Peritonitis. Prevention and cure.

Peritonitis. Prevention and cure.


Exotic infections as seen in travelers.

Exotic infections as seen in travelers.


The management of obesity: some recent concepts.


Mammotropic effect of fluphenazine enanthate in the rat.


Jaundice in urinary tract infection in infancy.


Changes in vascular permeability of the central nervous system by experimental herpes simplex and vaccinia virus infections.


Wegener's granulomatosis in a child. Observations on pathogenesis and treatment.


The advent of biosurgery.

The advent of biosurgery.


Some electrocardiographic changes in human heart transplants.


Histidine-2-oxoglutarate aminotransferase activity in Salmonella typhimurium.


3-methylhistidine metabolism in proteins from cultured mammalian muscle cells.


The properties of a nuclear acidic protein fraction that binds [6,7-3H]oestradiol-17beta. 1. Additional evidence was obtained that the nuclear oestradiol-17beta receptor is an acidic protein. Partial purification of the receptor protein was obtained by chromatography on hydroxyapatite and it contains protein-bound phosphate. 2. The nuclear ;5s' and cytoplasmic ;9.5s' and ;5s' receptors from uterus, dimethylbenzanthracene-induced mammary adenocarcinoma and kidney are precipitated together with bound oestradiol-17beta by protamine sulphate. This common property suggests that the nuclear and cytoplasmic receptors are related to each other. 3. The properties of two acidic protein fractions from both liver and dimethylbenzanthracene-induced mammary adenocarcinoma are described. Fraction 1 contains two major components and fraction 2 contains one component, as judged from polyacrylamide-gel electrophoresis. Fraction 2 contains RNA and both fractions contain protein-bound phosphate. 4. These fractions form insoluble complexes with calf thymus histone, protamine sulphate and poly-l-lysine. The formation of these complexes is markedly affected by ionic strength and pH. Ionization of both the in-amino group of lysine and carboxyl group are involved. RNA and DNA do not appear to be involved. The interaction is not affected by EDTA or 1mm-Na(+), -K(+), -Ca(2+), -Mg(2+) or -Mn(2+). Per unit weight, whole histone has 4-5 times as many binding sites for the acidic proteins as the latter have for the former. 5. No convincing evidence was obtained for DNA-acidic protein interaction, but, as judged from precipitation experiments, there was competition between DNA and acidic protein for histone. 6. Relatively large amounts of acidic protein partly relieved the histone inhibition of the template activity of DNA for Escherichia coli RNA polymerase (EC 2.7.7.6).


Studies of a phospholipid-requiring bacterial enzyme. II. The role of phospholipid in the uridine diphosphate galactose: lipopolysaccharide alpha-3-galactosyl transferase reaction.


Transcriptional and sedimentation properties of ribonucleic acid polymerase from Micrococcus lysodeikticus.


Transfer ribonucleic acid from Mycoplasma.


Evolutionary conservation of the synthetase recognition site of alanine transfer ribonucleic acid.


Unusual difference spectra of proteins containing tryptophan. II. Studies with proteins.


L-Asparaginase EC-2 from Escherichia coli. Some substrate specificity characteristics.


Kinetic properties of cobalt alkaline phosphatase.


Identification of episilon-N-methyllysine in Spirillum serpens flagella and of episilon-N-dimethyllysine in Salmonella typhimurium flagella.


The chemistry of malononitrile.

The chemistry of malononitrile.


Reactions of proteins, carbohydrates, and related substances in liquid hydrogen fluoride.


Repressible histidine transamination in Escherichia coli and its retro-inhibition.


The kinetics of catabolite repression in Escherichia coli.


Soluble protein profiles in Escherichia coli.


Study of the physiological state of microorganisms in continuous cultures.


Physiological properties of microbial population as a basis for graphical solution of multi-stage continuous cultivations.


Spinal dysraphism. A study of congenital malformations of the lower back.


The treatment of chronic osteomyelitis by saucerisation and secondary skin grafting.


Usher's syndrome--deafness and progressive blindness. Clinical cases, prevention, theory and literature survey.


AAMC responsibility for postgraduate education.


The role of the university in graduate medical education. Supplementary background information. 1. Graduate training programs.


André Mayer--a biographical sketch (1875-1956).


Unavailability of cystine from trypsin inhibitors as a factor contributing to the poor nutritive value of navy beans.


Inhibition of nitrogen mustard after-effect in F minus-cells by the factor produced by E. coli K-12 fertile strains.


Deletion mapping of galactose mutants with transducing lambda phages.


Protection and host cell repair of irradiated lambda phage. I. Irradiation of normal phage with ultraviolet light.


Protection and host cell repair of irradiated lambda phage. II. Irradiation of 5-bromouracil-substituted phage with near visible light.


Protection and host cell repair of irradiated lambda phage. 3. Ultraviolet irradiation of 5-bromouracil-substituted phage.


Infectious DNA from coliphage T1. I. Some properties of the spheroplast assay system.


Cytopathology of the cerebrospinal fluid examined with the sedimentation technique after Sayk.


Immediate and early functional adequacy of transplanted lungs.


Primary reconstruction of oropharyngeal surgical defects with a forehead flap.


One stage allogeneic transplantation of the liver and hepatectomy in dogs.


Immunosuppressive effect of antisera to subcellular lymphocyte fractions.


Management of contaminated surgical wounds.


Open mesencephalotomy and thalamotomy for intractable pain.


Bovine hyperkeratosis. (X-sease, highly chlorinated naphthalene poisoning) historical review.


Chronic alveolar emphysema in the horse.


Absorption of vitamin A.

Absorption of vitamin A.


Mineral dynamics during hibernation and chronic immobility: a review.


Spontaneous closure of ventricular septal defects. Anatomic evidence in six adult patients.


Detection of bovine viral diarrhea virus and antigen in tissues of experimentally infected calves by cell inoculation and fluorescent antibody techniques.


Trehalase activity in Selaginella martensii.


Effect of L-methionine and vitamin B 12 on methionine biosynthesis in Escherichia coli.


The study of cells in cerebrospinal fluid by electron microscopy. A new method.


Necrosis and regeneration of the tibialis anterior muscle in rabbit. I. Histological changes following the ligation of the tibialis anterior artery.


Erb's palsy.

Erb's palsy.


Quantitative evaluation of circulatory left-to-right shunts by ascorbate dilution using a single venous catheter for injection and measurement.


The effect of genetic complexity on the time-course of ribonucleic acid-deoxyribonucleic acid hybridization. 1. The rate of RNA-DNA hybridization was studied under conditions of RNA excess, with RNA synthesized in vitro. The initial rate of the reaction was proportional to the initial RNA concentration. Throughout the observed course of the reaction there was a linear relationship between the reciprocal of the amount of RNA hybridized/mug. of DNA and the reciprocal of time. The slope of the reciprocal plot was inversely proportional to the initial RNA concentration. 2. A comparison was made of the hybridization of DNA from Escherichia coli and from bacteriophages T4 and lambda with homologous RNA. The initial rate of hybridization was inversely proportional to the genetic complexity of the hybridizing system. The slope of the reciprocal-time plot was directly proportional to genetic complexity. These results are interpreted to indicate that the rate of hybridization reflects the mean concentration of the various unique RNA species in a preparation.


Historic milestones in mental retardation. Random notes and sketches.


Robert Debré--pediatrician.

Robert Debré--pediatrician.


Streptozotocin diabetes in the mouse and guinea pig.


Insulin storage and release in rats bearing growth hormone secreting tumors.


Cardiovascular effects of cold pressor tests, 40 degrees head-up tilt, and smoking on smokers and non-smokers.


Evaluation of the reimplanted lung by lung scanning in dogs.


The artificial heart: research, development or invention?


Anesthetic management of the parturient in hemorrhagic shock.


Anesthetic management of the parturient in septic shock.


Max Brödel (1870-1941).

Max Brödel (1870-1941).


A double blind study of sulfa prophylaxis after transurethral resection.


In memoriam. Dr. Zakir Husain.

In memoriam. Dr. Zakir Husain.


Temperature-sensitive mutants of Mycoplasma pneumoniae. I. In vitro biologic properties.


Quantitative bacterial sensitivity: antibiotic gradients and probable in vivo response.


Spontaneous reticular neoplasms in (CBA X DBA/2)F1 mice, with special emphasis on the occurrence of plasma cell neoplasms.


The in vitro transformation by an avian adenovirus (CELO). 3. Human amnion cellcultures.


Cancer of the uterine cervix in mice fed a liquid diet containing an antifertility drug.


Thermogravitational effect in macromolecular solutions.


A histochemical study of the course and distribution of the efferent vestibular fibers to the maculae of the saccule and utricle.


Revascularized skin flaps: a new concept in transfer of skin flaps.


Directory of otolaryngologic societies.

Directory of otolaryngologic societies.


Decision.

Decision.


The place of heterologous antilymphocytic globulin in renal transplantation.


Leukocyte antigens in renal transplantation. 8. Successful renal transplantation in the presence of a positive cross match.


Leukocyte antigens in renal transplantation. 7. Immediate renal allograft rejection with a negative cross match.


Clinical experience with glynclamide, a new hypoglycaemic agent.


"Elliptical" excision and suture.

"Elliptical" excision and suture.


Experimental studies relating to the production and clinical testing of anti-human lymphocyte serum.


Veterans multiclinic trial of drug treatment in hypertension.


Coagulation studies in the hyperacute and other forms of renal-allograft rejection.


The flow of lymph.

The flow of lymph.


Innocence without virtue--graft-versus-host disease in man.


Post-transplantation malignancy.

Post-transplantation malignancy.


Microangiopathic hemolytic anemia.

Microangiopathic hemolytic anemia.


Establishing the facts.

Establishing the facts.


The puzzle of pain.

The puzzle of pain.


Treatment of gram-negative infections in patients before and after renal transplantation.


Autoimmunity to heart and its relation to heart disease.


Cerebrospinal fluid transport.

Cerebrospinal fluid transport.


Influence of elevated circulating levels of amino acids on cerebral concentrations and utilization of amino acids.


Transport as control mechanism of cerebral metabolite levels.


The development of the blood-brain barrier.


Delineation of fluid compartmentation in cerebral tissues.


The fine structure of the choroid plexus adult and developmental stages.


Histocompatibility in man--genetic and practical considerations.


The biosynthesis and metabolism of the catecholamines.


Role of O2 and N2 in thermorestoration of hdrated bacterial spores.


Biological determination of the germicidal activity of sunlight.


Effect of small doses of syngeneic bone marrow on the 30- and 90-day LD50 of X-irradiated mice.


Dynamic studies with the newer instrumentation.


Tumor immunology and response to radiation therapy.


Eastern encephalitis: a review of recent studies in New Jersey.


Polycyclic aromatic hydrocarbons (polynuclears) in smoked foods.


Screening methods for organochlorine and organophosphate insecticides in foods and feeds.


Preliminary trial of a powerful new sulphonylurea in maturity-onset diabetes--HB419 (glibenclamide).


Clinical trial of a new sulphonylurea in maturity-onset diabetes--HB419 (glibenclamide).


Human placental lactogen (HPL): the review of a protein hormone important to obstetrics and gynecology.


Elimination of disruption of abdominal incisions.


Lung transplantation with simultaneous contralateral pulmonary artery ligation.


Transplantation of cadaver organs.

Transplantation of cadaver organs.


Two fatal cases of Plasmodium falciparum malaria contracted in tropical Africa.


Preventive effect of pyridinolcarbamate on thrombohemorrhagic phenomenom and hemorrhagic necrosis.


Falciparum malaria in England--an unusual case.


Preliminary investigations into the incubator-vector status of the cockroach Blatta orientalis.


Quantitative assessment of bacterial contamination of open wounds by a slide techniue.


In vivo and in vitro chelation of plutonium by alpha-lipoic acid and DTPA. BNWL-714.


An automated method for polyamine analysis.


[Studies on the action of free fatty acids on the microflora of salt pork meats].


[Use of immunofluorescence in the titration of antibodies neutralizing the virus of classic swine plague].


Kinetics of microbial oxidation of n-heptane. I. Characterization of the process.


The conversion of benzyl penicillin to 6-aminopenicillanic acid using an insoluble derivative of penicillin amidase.


Reproducibility of body composition measurements in a single individual.


Uraemic constrictive pericarditis.

Uraemic constrictive pericarditis.


A controlled trial of imipramine, amphetamine, pad-and-bell conditioning and random awakening in the treatment of nocturnal enuresis.


Haematology in my time.

Haematology in my time.


A new anti-emetic for mustine nausea.

A new anti-emetic for mustine nausea.


Pregnancy and the lower urinary tract. 3. The surgical treatment of urinary stress incontinence resulting from pregnancy.


Infantile cortical hyperostosis (Caffey's disease).


Isolation of tick paralysis toxin from Ixodes holocyclus.


Foundations of child psychiatry.

Foundations of child psychiatry.


The children haven't read those books, reflections on differential diagnosis.


Carleton Mathewson, Jr.: inspiration for excellence.


Tributes to Carleton E. Mathewson, Jr. from the City and County of San Francisco Department of Public Health.


Carleton Mathewson, Jr. of Sanford.

Carleton Mathewson, Jr. of Sanford.


A man to match a mountain. Carleton Mathewson, Jr. at the University of California.


Friend, citizen-soldier, and military surgeon.


The City and County Hospital of San Francisco. Recollections of far away and long ago.


Oxygen and healing.

Oxygen and healing.


Methylmalonic acid and coenzyme A concentrations in the livers of pair-fed vitamin B 12-deficient and vitamin B 12-treated sheep. The concentrations of CoA in the livers of severely vitamin B(12)-deficient ewes were about 2.6 times those in pair-fed animals treated with vitamin B(12). When the feeding rates of the pair-fed animals were closely similar, the concentrations of methylmalonic acid in deficient livers were about twice those in vitamin B(12)-sufficient livers. The molar concentrations of CoA present were more than three times those of methylmalonic acid in both deficient and treated animals, and it is concluded that the elevated concentrations of CoA in the deficient livers were not primarily due to accumulation of methylmalonyl-CoA.


Regulation of erythropoiesis (XXIV). Studies on the post-hypoxic "rebound" phase.


Central nervous system complications of leukemic conversion of the lymphomas.


Epidemiologic and histologic study of oral cancer and leukoplakia among 50,915 villagers in India.


Juvenile hemangioendothelioma of the liver. Report of a case and review of the literature.


On the occurrence of Clostridium botulinum type C beta in the livers of slaughter animals in Denmark.


The annual George C. Griffith scientific lecture.


The eponyms and vagaries of sarcoidosis.


The effect of environment on skeletal tissue in culture.


Experimental reversal of germ cells.

Experimental reversal of germ cells.


On diluent and dilution method in hemagglutination inhibition test for human chorionic gonadotropin.


A new technique for electron microscopic dry-mounting radioautography of soluble-compounds.


Ultrastructural localization of zinc in rat exocrine pancreas by autoradiography.


The induction of prolonged resistance against a lethal staphylococcic infection in mice with a single injection of an emulsified lipopolysaccharide.


Detection of an organ-specific population of RNA in the salmon brain.


Development of neuronal connections with skin grafts in frogs: behavioral and electrophysiological studies.


Distribution and termination of spinoreticular afferents in the brain stem of sheep.


Penicillin beta-lactamase and penicillin acylase formation by gram-negative bacteria.


John Cruickshank, 5 August 1881-10 Oct 1966.


A fluorescent antibody technique for studying thymocyte migration in the rat.


Acrylic aldehyde in sodium dichromate as a fixative for identifying catecholamine storage sites with the electron microscope.


Storage of livers for transplantation.

Storage of livers for transplantation.


What is a scholar?

What is a scholar?


Degeneracy in the secondary immune response: stimulation of antibody formation by cross-reacting antigens.


The xenograft rejection phenomenon. II. Response of the guinea pig to mouse, rat and rabbit skin xenografts.


Fate of osteogenic tissue transferred to the subcutaneous area by means of polyvinyl-formal sponge.


Asymptomatic bacteriuria in 200 women undergoing uterine curettage following abortion.


Sonne dysentery in New Zealand.

Sonne dysentery in New Zealand.


University of Otago 1869-1969.

University of Otago 1869-1969.


Mapping of the aspartase gene in Escherichia coli K-12.


James Joyce's Ulysses.

James Joyce's Ulysses.


Mode of action of antilymphocyte serum.

Mode of action of antilymphocyte serum.


Heterogeneity of animal hemoglobins.

Heterogeneity of animal hemoglobins.


Aortic dissecting aneurysms.

Aortic dissecting aneurysms.


Canine ehrlichiosis.

Canine ehrlichiosis.


Histomoniasis.

Histomoniasis.


Changes in the relationship of nasion, point A, and point B and the effect upon ANB.


Inhibition of granuloma formation around Schistosoma mansoni eggs. V. "Hodgkin's-like lesion" in SJL-J mice.


Blastic reaction of the regional lymph node as a method for quantitative evaluation of tissue incompatibiliy.


Diffusion of plasma proteins into cells. A manifestation of cell injury in rabbit skeletal muscle exposed to lecithinase C.


Beta cell morphology and insulin immunoassay. In long-term subdiabetic rabbits.


The fine structure of the Marinesco body.


Evaluation of three isotope-dilution techniques for studying the kinetics of glucose metabolism in sheep. 1. Comparisons have been made of three isotope-dilution techniques for measuring parameters of glucose metabolism in sheep given their daily ration in 12 equal amounts (i.e. from 07.00 to 18.00hr.) 2. [U-(14)C]Glucose was used in all experiments. After a single injection the specific radioactivity of plasma glucose was measured at specific times for up to 24hr. Primed infusions were made with various ratios of P, priming injection (nc), to F, infusion rate (nc/min.) (P/F ratios varying from 23:1 to 147:1) and the specific radioactivity of plasma glucose was measured at 60, 120, 150, 180, 210 and 240min. In continuous infusions the specific radioactivity of plasma glucose was followed for 9hr.; a constant specific radioactivity was observed after approximately 180min. 3. A computer programme was used to fit a multi-exponential equation to the log(specific radioactivity)-time curve after a single injection. A second- or third-order exponential equation was found to fit the results. 4. Conventional analyses of all results showed that similar estimates of the irreversible loss of glucose were obtained by using all three techniques. Estimates of glucose pool size and space by using the primed infusion technique were both significantly higher than estimates obtained by the single injection technique. In these experiments total entry rate could only be determined from the single-injection results and a wide variation in estimates was obtained. 5. Comparisons of the specific radioactivity-time relationships after a single injection of [U-(14)C]glucose in sheep given their ration either once daily or as a proportion at hourly intervals indicated that there were fluctuations in glucose synthesis in the former over the period of the experiment. The multi-exponential curves fitted to these results had larger residual variances than in sheep given food at hourly intervals. All parameters of glucose metabolism estimated were similar under both feeding regimes. 6. A number of methods of analysis are discussed and a model for glucose metabolism in sheep in suggested.


[Fidelity of polyuridylic acid translation in mixed systems derived from Bacillus stearothermophilia and Escherichia coli].


Completion of the amino acid sequence of papain. Papain was inhibited with bromo[2-(14)C]acetic acid, the tertiary structure of the inhibited enzyme was unfolded and the disulphide bridges were reduced with mercaptoethanol and aminoethylated. Digestion with trypsin gave a radioactive peptide consisting of residues 18-58 inclusive and containing therefore the sequence of the thirteen unknown residues 29-41 in the primary sequence of papain. This peptide was digested with pepsin to give a radioactive peptide consisting of residues 18-47, which after digestion with 0.4m-hydrochloric acid gave a radioactive peptide consisting of residues 24-43 inclusive. Further digestion with 6m-hydrochloric acid gave peptides that were used to determine the sequence: Ser-Ala-Val-Val-Thr-Ile-Glx-Gly-Ile-Ile-Lys-Ile-Arg for the residues 29-41, so completing the amino acid sequence of papain.


Studies of a phospholipid-requiring bacterial enzyme. I. Purification and properties of uridine diphosphate galactose: lipopolysaccharide alpha-3-galactosyl transferase.


Nitroso compounds.

Nitroso compounds.


Body-temperature and responses to drugs.


Liver transplantation in man after an extended period of preservation by a simple technique.


Stereotactic recording from the spinal cord of man.


Completely mature pulmonary metastasis from testicular teratocarcinoma. Case report and review of literature.


Chemotherapy in aged psychiaric patients.


Infections of the vagina and vulva.

Infections of the vagina and vulva.


Biologic significance of piezoelectricity.


Antibiotics in pelvic infections.

Antibiotics in pelvic infections.


Stroke U.S. & Japan. Serum lipid profiles.


Ocular manifestations of strokes.

Ocular manifestations of strokes.


An unsolved problem. Low serum PBI values in patients with chronic disease.


Genetic heterology between Escherichia coli K12 and a smooth strain of E. coli.


Action of hydrogen peroxide on growth inhibition of Salmonella typhimurium.


The cause of loss of viability of airborne Escherichia coli K12.


Split-dose irradiation of Escherichia coli in the absence and presence of mercaptoethylamine.


A hypothesis for the pathogenesis of the generalized Shwartzman reaction.


The new Assistant Secretary for Health and Scientific Affairs.


Analysis of pressures produced during maxillary edentulous impression procedures.


Pharmaceutical sciences--1968. A literature review of pharmaceutics.