The burns unit in Copenhagen. I. Principles of treatment.


Restoration of the blood-supply to human skin autografts.


Transplantation between monozygotic twins.


Electrical hand burns.

Electrical hand burns.


Polypeptide chain elongation in protein biosynthesis.


Nucleoside triphosphate termini from RNA synthesized in vivo by Escherichia coli. Alkaline hydrolyzates of RNA made in vivo by Escherichia coli contain ribonucleoside-3'-monophosphate-5'-triphosphates. These probably arise by hydrolysis of the initial nucleoside triphosphate from the 5' terminus of the nascent RNA chains. Logarithmically growing cultures, labeled for 45 seconds with (32)P-labeled phosphate, yield about 2000 molecules of labeled tetraphosphate per cell, this yield increasing only slightly with continued labeling. Only the tetraphosphates of adenosine and guanosine have been found in Escherichia coli, and these two are present in approximately equal amounts.


Lactate dehydrogenase isozymes in human cardiac transplantation. Lactate dehydrogenase (LDH) isozyme activities have been followed in 17 human cardiac allografts. A pattern of abnormality associated with cardiac rejection during the first month after operation has been determined: (i) LDH-1 activity is greater than LDH-2 activity; (ii) LDH-1 activity is greater than 35 percent of total LDH activity; and (iii) LDH-1 activity is greater than 100 international units. The LDH-1 abnormality helps to meet the need for an index of cardiac rejection during the early weeks after operation when the electro-cardiogram is least reliable.


Antiserum to lymphocytes and procarbazine compared as immunosuppressants in mice. As an immunosuppressant procarbazine was as potent as an antiserum to lymphocytes in mice. Both agents significantly prolonged survivals of skin allografts by 4 to 6 weeks. At the maximum tolerated dosage, procarbazine suppressed both circulating hemagglutinins to sheep erythrocytes and plaque-forming cells in the spleen. The antiserum to lymphocytes reduced the numbers of circulating hemagglutinins and plaque-forming cells by 94 percent. Procarbazine was administered to mice for 3 weeks before their exposure to antigen. Exposure for only 9 days, commencing 48 hours before injection of sheep erythrocytes, was less effective in suppressing the titers of hemagglutinins.


Jacob Fine: academic surgeon. Three decades of research.


Transplantation in the future.

Transplantation in the future.


The influence of the uraemic state on lymphoid tissue with particular reference to the homograft reaction.


The storage of organs for transplantation.


Some considerations on the healing of intestinal anastomoses.


Buerger's disease revisited.

Buerger's disease revisited.


Cell specialization in shoot apical meristems as related to radiosensitivity and histogenesis. ORO-661.


Physiological studies on Clostridium botulinum, type F. Three year summary report of progress, March 1, 1965--November 30, 1967. ORO-3347-5.


Physiological studies on Clostridium botulinum, type F. Progress report, December 1, 1966--November 30, 1967. ORO-3347-4.


Growth characteristics of type E Clostridium botulinum in the temperature range of 34 degrees F to 50 degrees F. Annual report, June 29, 1962-June 28, 1963. TID-24778.


Dizygotic cattle twins and tissue tolerance. ORO-661.


Stannous fluoride (0.1 percent) mouthwash study. SAM-TR-68-78.


Fractures of the calcaneum. Open reduction and bone grafting.


Diagnostical value of the complement fixation test and the fluorescent antibody method in the laboratory diagnosis of toxoplasmosis.


Denture identification.

Denture identification.


A model for comparing the optical properties of different-sized corneal grafts.


A control test of a new microbiological testing device under office conditions.


Human rabies encephalitis. Fine structure study of cytoplasmic inclusions.


Fornix-based conjunctival flap in the treatment of corneal ulcers.


Tay-sachs disease. Demonstration of the stored ganglioside in cultured cells from brain biopsy.


Constancy of uptake during the cell cycle in Escherichia coli. Rates of uptake of several labeled compounds were measured during the cell cycle for three strains of Escherichia coli in balanced growth. Uptake rates were constant during more than the first two-thirds of the cycle, or reasonably so, for all of these compounds: glycine, leucine, glucose, acetate, phosphate, sulfate, and thymidine. When added de novo, uptake of glycine and leucine were not constant, but appeared to be proportional to mean cell volume. These results are in agreement with the finding that cell sizes increase linearly during most of the cell cycle for E. coli. They support the hypothesis, for cultures in balanced growth, that linear growth during the cell cycle is due to constant rates of uptake of all major growth factors. They also support the interpretation that uptake is limited by the presence of a constant number of functional binding or accumulation sites for these growth factors.


Metronidazole as an adjuvant in the treatment of alcoholism.


Historical note--hashish and alcohol "scenes" in France and Great Britain 120 years ago.


Centenarius Scandinavicus.

Centenarius Scandinavicus.


Serotyping for homotransplantation. XXII. Specificity of cytotoxic antibodies developing after renal transplantation. Cytotoxic antibodies against allogeneic lymphocytes were detected in 13 patients after removal of a renal allograft. Antibodies were not detected in seven patients who had grafts in situ for less than two months, but were detected in 13 out of 17 patients who had grafts in situ for longer than two months. The HL-A specificity of these positive sera was compatible with the HL-A antigen mismatch of the donor-recipient pairs in most instances, providing further evidence that such antibodies result from direct sensitization by the allograft and may play some part in renal allograft rejection.


Hypophosphataemia in septicaemia: higher incidence in gram-negative than in gram-positive infections. Phosphataemia has been investigated in 54 patients with Gram-negative septicaemias; either absolute (serum phosphate <2 mg./100 ml.) or relative (P./B.U.N. 0.04) hypophosphataemia was found in 69% of all determinations. In 37 patients with Gram-positive septicaemias hypophosphataemia was present in only 24%, many of whom had additional Gram-negative bacteriuria and possible Gram-negative septicaemia suppressed by antibiotics. The mechanism of this phenomenon is not known. This new sign of Gram-negative septicaemia has some diagnostic importance.


Nephrotic syndrome due to primary renal disease in adults: II. A controlled trial of prednisolone and azathioprine. A controlled trial in 20 adult patients with the nephrotic syndrome due to proliferative glomerulonephritis compared the effects of consecutive eight-week courses of treatment with prednisolone in conventional dosage and a low-dose azathioprine-prednisolone combination. Though the azathioprine regimen avoided serious toxicity and produced a statistically significant improvement in creatinine clearance and urine protein excretion, the results were not significantly better than with prednisolone itself and overall were not of great clinical value. Prolongation of the azathioprine-prednisolone treatment to one year was associated with some small improvement in the results.The effectiveness of prednisolone in the nephritic syndrome of adults with minimal renal histological abnormality was confirmed by a complete loss of proteinuria within eight weeks in six out of eight patients and substantial reduction in the other two. These two patients subsequently received azathioprine as well but with equivocal results.


Cadaveric kidneys for transplantation.

Cadaveric kidneys for transplantation.


R. T. Leiper.

R. T. Leiper.


Acute infective gastroenteritis.

Acute infective gastroenteritis.


T. L. Hardy.

T. L. Hardy.


R. S. Weetch.

R. S. Weetch.


T. N. Morgan.

T. N. Morgan.


P. D. Wilson.

P. D. Wilson.


The localization of endogenous "Prolactin" in the pituitary gland of the goldfish, Carassius auratus, Linnaeus.


Studies on the mode of action of the heterologous immunogenicity of a methanol-insoluble fraction of attenuated tubercle bacilli (BCG).


Rapid antibiotic sensitivity test.

Rapid antibiotic sensitivity test.


The effect of rabbit vesical mucosa on bacterial growth.


Glycosuria and the growth of urinary pathogens.


The development of the cystoscope. From "lichtleiter" to fiber optics.


Phosphoenolpyruvate carboxylase of Salmonella. Some chemical and allosteric properties.


Succinyl phosphate and the succinyl coenzyme A synthetase reaction.


Formation of ribosylthymine in Escherichia coli. Studies on pulse labeling with thymine and thymidine.


Arginine transport and metabolism in osmotically shocked and unshocked cells of Escherichia coli W.


Stimulation of arginine transport in osmotically shocked Escherichia coli W cells by purified arginine-binding protein fractions.


Borohydride reduction of phenylalanine transfer ribonucleic acid. Effect on enzyme recognition.


Further studies on the dialyzability of bacteria.


Albert Gallatin Mabry.

Albert Gallatin Mabry.


Senator Hill and his contributions to health sciences.


A technique for the study of acetylcholine turnover in mouse brain in vivo.


Diagnosis of pericardial effusion by radioisotopic angiocardiography.


Bone graft closure of large or persistent oromaxillary fistula.


Developmental variations of the temporal bone that influence the evolution of chronic suppurative otitis media and mastoiditis and the medical and surgical treatment of this syndrome.


Fixation in vivo by preassessed intralabyrinthine perfusion in cats' inner ears.


Revascularization of the larynx.

Revascularization of the larynx.


Mattress suture otoplasty: indications and limitations.


Studies on radiation-sensitive mutants of E. coli. 3. Participation of the rec system in induction of mutation by ultraviolet irradiation.


Isolation of conjugation-constitutive mutants of colicin factor Ib.


Renal role in Goodpasture's syndrome.

Renal role in Goodpasture's syndrome.


Platelet function.

Platelet function.


Selection of organ donors.

Selection of organ donors.


Genetic evolution of bacteriophage. I. Hybrids between unrelated bacteriophages P22 and Fels 2. A new bacteriophage species, designated F22, was isolated from phage P22 stocks grown on Salmonella typhimurium Q1 lysogenic for Fels 2 at a frequency of less than 10(-11). P22 has a very short tail with a hexagonal base plate and six spikes. Phage Fels 2 is morphologically similar to E. coli T-even phages, having a long tail with a contractile sheath and carrying no genetic region related to P22. Phage F22 is morphologically and serologically indistinguishable from Fels 2, but carries the c(c(1), c(2), and c(3)) markers of P22. The color markers h(21), g, and m(3) of P22 do not appear in F22. Thus, F22 is evidently a recombinant between the unrelated bacteriophages P22 and Fels 2. The recombination between unrelated bacteriophages could play an important role in the evolution of bacteriophages.


Insulin biosynthesis in the rat: demonstration of two proinsulins. Proinsulin, a single-chain precursor of insulin, has been detected in biosynthetic studies in vitro,(1-4) and in crystalline preparations of insulin from several species.(1, 5-7) It appears that proinsulin normally serves neither as a major storage nor secretory form of the hormone.(8) Its primary functions seems to be to facilitate the efficient formation of the disulfide bonds of insulin.(9)Although several species of fish have two insulins differing slightly in primary structure,(10-12) the rat is the only mammal where this is known to be the case.(13, 14) As the two rat insulins are nonallelic(14) and presumably coded by two distinct genes, a separate proinsulin would be expected to precede each insulin. We have demonstrated the biosynthesis of both insulins(15) and of a proinsulin corresponding to each in isolated islets of Langerhans, and have detected probable intermediates in the conversion of these proinsulins to insulin. Labeled peptide material corresponding to the free connecting segment of proinsulin (C-peptide) was detected in insulin-containing fractions isolated from incubated rat islets. It was noted that islets incubated in vitro secreted small amounts of newly synthesized proinsulin as well as the two insulins and essentially equivalent amounts of C-peptide. Increasing the glucose content of the medium selectively enhanced the incorporation of radioactivity into proinsulin and insulin and also seemed to increase the relative proportion of labeled proinsulin appearing in the medium.


Delayed hypersensitivity induced in guinea pigs with tuberculoprotein from M. bovis (BCG).


Relationship of spermidine and nucleic acids to growth in Lactobacillus casei and Escherichia coli.


Strain differences in the liver autoantibody response of inbred mice.


Protection of neonates against whole-body radiation by the administration of a single emulsified injection of a lipopolysaccharide during pregnancy.


Cytokinesis in HeLa: post-telophase delay and microtubule-associated motility.


Modified dye dilution technique for cardiac output studies in tiny subjects.


How normal is the donor heart?

How normal is the donor heart?


Speculation on the function of the transplanted heart.


Why we are here. A tribute presented at the dedication of the Kenneth F. Maxcy Laboratories.


Does a graft versus host reaction occur between fetus and mother?


Isolation of Histoplasma capsulatum from Arizona bats.


Harry Harris. The William Allan Memorial Award presented at the annual meeting of the American Society of Human Genetics, Austin, Texas October 12, 1968.


The centennial: an interpretation.

The centennial: an interpretation.


Observations on living oogonia and oocytes from human embryonic and fetal ovaries.


Responsibility of the obstetrician-gynecologist for lay education.


The pineal gland in relation to reproduction.


Chromosomal errors and development.

Chromosomal errors and development.


Salvage of the reproductive potential of women with anomalous development of the Müllerian ducts: 1868-1968-2068.


Human fetal nutrition and growth.

Human fetal nutrition and growth.


The influence of maternal folate and iron deficiencies on intrauterine life.


Placenta and fetal tissues: a biphasic system for the synthesis of steroids.


Surface mucin of human trophoblast.

Surface mucin of human trophoblast.


Skin allograft survival and lowered maternal immunologic reactivity.


A review of carbohydrate metabolism and the oral contraceptives.


Treatment of the acute alcohol withdrawal state: a comparison of four drugs.


The future of the public mental hospital.


The dreams of Benjamin Rush.

The dreams of Benjamin Rush.


Esophagitis: diagnosis and surgical treatment.


Cardiac transplantation: general considerations and results.


Replacement of diseased aortic valves with homografts.


A double blind study of the effect of tranexamic acid in essential menorrhagia.


Jozef Strus of Poland (1510-1568).

Jozef Strus of Poland (1510-1568).


Study of luminal coronary collateral circulation in the beating canine heart.


The YY chromosome complement. What does it mean?


Cellular basis of morphogenetic movement.


The founding of the American Pediatric Society. A landmark in pediatrics.


Minute Y chromosome in the lizard genus Uta (family Iguanidae).


The relation between virus-like particles and R bodies of Paramecium aurelia.


The sequence of four structural and two regulatory methionine genes in the Salmonella typhimurium linkage map.


An interview with Robert Q. Marston, M.D.


A study on anti-pituitary serum.

A study on anti-pituitary serum.


Opsonic, cytophilic and agglutinating activity of guinea-pig gamma-2 and gamma-M anti-Salmonella antibodies.


Regulation of antibody synthesis against Escherichia coli endotoxin. 3. Induction of immunological paralysis in non-immune and pre-immunized mice.


Cytotoxic effects in vitro by lymphoid cells from specifically tolerant animals.


The complement lysozyme sequence in immune bacteriolysis.


Regulation of antibody synthesis against Escherichia coli endotoxin. II. Specificity, dose requirements and duration of paralysis induced in adult mice.


Anterior cervical discectomy and fusion. A clinical and biomechanical study with eight-year follow-up.


Dislocation and deformity of the hip in children with spina bifida cystica.


The growth of bone autografts in rabbit ear chambers.


J. M. P. Clark.

J. M. P. Clark.


Antibody response in rainbow trout (Salmo gairdneri) I. Immunocompetent cells in the spleen and anterior kidney.


Alterations in immune responses by attenuated Venezuelan equine encephalitis vaccine. II. Pathology and soluble antigen localization in guinea pigs.


The antibody response of guinea pigs to topical and intramuscular administration of trachoma antigens.


Cytophilic antibody in heterologous antilymphocytic serum: its presence, absorption and possible biologic significance.


Clinical measurement of plasma-protein synthesis.


Ultrasonic effects affecting the mechanism of reproduction of micronized microorganisms.


Males with an XYY sex chromosome complement.


Surgical repair of subaortic annular aneurysm.


Pavlov and genetics.

Pavlov and genetics.


Agranulocytosis induced by certain phenothiazine derivatives.


Skeletal complications in a series of human renal allografts.


Johann Friedrich Horner, (1831-1886) "a form of ptosis".


Croup.

Croup.


The romance of Charles H. Liebermann, MD.


HEW study just released concludes chiropractor can't provide quality care.


Repair of lesions induced by photodynamic action and by ethyl methanesulfonate in E. coli.


Ribosomal proteins. V. Comparison of protein patterns of 70S and 80S ribosomes from various plants by polyacrylamide gel electrophoresis.


Mutagenesis in Escherichia coli. II. Evidence for a common pathway for mutagenesis by ultraviolet light, ionizing radiation and thymine deprivation.


Bacterial genetic factors controlling the suppression of T4 phage amber mutants. I. Suppression patterns of a collection of E. coli strains.


Increase in antigenicity of permanent tissue culture lines ICI 101, ICI 104 and ICI 202 established from human leukaemic blood.


Bacterial genetic factors controlling the suppression of T4 phage amber mutants. II. Suppression patterns among the segregants of bacterial crosses.


Bone-marrow transplantation after antilymphocytic serum and lethal chemotherapy.


Recent developments in transplantation immunology. I.


Nonhormonal treatment of the menopausal syndrome. A double-blind evaluation of an autonomic system stabilizer.


Conchal cartilage grafts in augmentation rhinoplasty and orbital floor fracture.


Problems in reconstruction of the auricle.


Development of plastic surgery from ancient times to the end to the eighteenth century.


An older version of the new economical dermatome.


Functional determination of the flexor tendon graft length. Modification of author's original technique.


Use of rubber bands in tie-over dressings on the chest wall.


James Hinton.

James Hinton.


The Reverend Joseph Townsend MA MGS (1739-1816) physician and geologist--'Colossus of Roads'.


Escherichia coli and ulcerative colitis.


Kergaradec, friend of Laennec and pioneer of foetal auscultation.


Incisional hernia.

Incisional hernia.


Urology yesterday, today and tomorrow.

Urology yesterday, today and tomorrow.


Structural determinant of a ribosomal protein: K locus. One of the ribosomal proteins (30S-8B) of Escherichia coli B has a greater electrophoretic mobility than the homologous protein (30S-8K) in E. coli K. The amino acid compositions, tryptic peptides, and cyanogen bromide peptides of these two homologous proteins unambiguously indicate that the genetic locus which determines the electrophoretic mobility of protein 30S-8 is the structural gene for this protein.


Lesch-Nyhan mutation: prenatal detection with amniotic fluid cells. Cells cultured from the amniotic fluid of a 22-week fetus in a heterozygote for the X-linked Lesch-Nyhan mutation, which results in neurological and developmental disorders, lacked sex chromatin and were unable to incorporate hypoxanthine. The diagnosis of a mutant male was confirmed upon birth of enzyme-deficient, hyperuricemic twin boys whose amniotic membrane cells failed to incorporate hypoxanthine.


The use of propranolol (inderal) in acute myocardial infarction.


Comparative evaluation of the thoracic impedance and isotope dilution methods for measuring cardiac output.


Modification of deoxyribonuclease test medium for rapid identification of Serratia marcescens.


Controlled-release iron therapy in pregnancy.


Amniotic fluid electrolytes and perinatal outcome.


Modifications of albumin transport capacity in pregnant women and newborn infants.


Pathology--the doorway to the understanding of leprosy. Ward Burdick Award Address.


Observations on the cellular response to filarial skin test antigen using the "skin window" technique: a preliminary report.


Mycobacterium leprae and its transmission in animals.


Nalidixic acid in urinary infection.

Nalidixic acid in urinary infection.


Freeze-etching simplified.

Freeze-etching simplified.


Low temperature as an environmental stress on microbial enzymes.


Distribution of C14-labeled dimethyl sulfoxide in tissues of intact animals.


Genetic studies of strain Bs8 of Escherichia coli.


Genetic analysis of some mutations causing resistance to tetracycline in Escherichia coli K12.


Multistep transduction of tryptophan (trp) genes in Escherichia coli.


The use of quinestradol in elderly incontinent women, a preliminary report.


The morphology of immune reactions in normal, thymectomized and reconstituted mice. I. The response to sheep erythrocytes.


Effect of tritiated thymidine and 5-bromodeoxyuridine on development of immunologically competent lymphocytes.


Evolution of cardiology with particular reference to congenital anomalies of the heart through the ages.


The influence of molecular diffusion on the dispersion of indicators in the circulation.


A most learned country doctor. Francis Adams (1796-1861).


William Carlos Williams, M.D. (1883-1963). Physician as poet.


Magnesium sulfate as circulation time indicator. Comparison with objective indicator radioactive iodine.


Artificial respiration in Biblical times.


Autoimmunity in pernicious anemia.

Autoimmunity in pernicious anemia.


Mycoplasma-like forms and immunofluorescent antibodies in cytoplasm and intercellular spaces of rete malpighii in pemphigus vulgaris and pemphigus vegetans.


American college of cardiology.

American college of cardiology.


Alcoholic cardiomyopathy.

Alcoholic cardiomyopathy.


Principles of nasal septum reconstruction.


The origin of mononuclear cells in delayed hypersensitivity reactions.


Cosmetic concepts in scar camouflaging--serial excisional and broken line techniques.


Reconstructive procedures on the auricle.


The pharmacology of the antimalarials. A rational approach to the therapy of resistant falciparum malaria.


An outbreak of Serratia marcescens, and its control.


Nerve suturing.

Nerve suturing.


Surgical techniques for the vocal rehabilitation of the postlaryngectomized patient.


Graft rejection.

Graft rejection.


Comparison of chlorambucil and streptonigrin (NSC-45383) in the treatment of malignant lymphomas.


Lymphographic study of giant follicular lymphoma.


An objective evaluation of haloperidol in Gilles de la Tourette's syndrome.


In memoriam: Titus Holiday Harris.

In memoriam: Titus Holiday Harris.


Correlation of unit activity in the hypothalamus with EEG patterns associated with the sleep cycle.


Our Philadelphia ophthalmic heritage: Dr. Fitzwilliam Sargent and Dr. George B. McClellan.


Bronchiectasis past and present.

Bronchiectasis past and present.


History of legal and medical roles in narcotic abuse in the U.S.


Inhalation therapy in the treatment of chronic obstructive lung disease.


Long term use of acetohexamide in maturity-onset diabetes mellitus.


Use of urea in the herniated intervertebral disc syndrome. A preliminary report.


Uterine perforation with an intrauterine device. Case report and review of literature.


Uptake of exogenous horseradish peroxidase by coated vesicles in mouse neuromuscular junctions.


A simple method for determination of glutaraldehyde.


W.M. Court Brown.

W.M. Court Brown.


Surgical correction of ureteral injury.

Surgical correction of ureteral injury.


Primary sarcoma of the urogenital system in adults.


The case against "the case against the catheter": initial report.


Stigmata: where mind and body meet. A study of autoerythrocyte sensitization.


Methadone treatment of randomly selected criminal addicts.


Transplantation of cadaveric kidneys from patients with hepatorenal syndrome. Evidence for the functionalnature of renal failure in advanced liver disease.


The neighborhood health center--reform ideas of yesterday and today.


Effects of vitamin D and steroid hormones on the active transport of calcium by the intestine.


Renal resurrection.

Renal resurrection.


Problems of planning.

Problems of planning.


Adverse conditions found in the use of ultra high-speed equipment.


Dental calculus: Chemical inhibition and removal. A review of the literature.


Endodontic treatment of vital and non-vital immature teeth.


Oral proteolytic enzymes (pancreatic) in traumatic dental surgery. A 2 part double-blind study.


Neighboring-group participation in sugars.


Fred Smith. 1911-1965.

Fred Smith. 1911-1965.


D-fructose and its derivatives.

D-fructose and its derivatives.


Acid-catalyzed hydrolysis of glycosides.


X-ray structure opolysaccharides.

X-ray structure opolysaccharides.


The thermal degradation of starch.

The thermal degradation of starch.


The ultrastructure of the cytoplasm of the developing amphibian egg.


Embryonic determination of neural connections.


Treatment of streptococcal infection with sulfamethoxazole and penicillin. Bacteriological and immunological response.


An optimistic and aggressive approach to the treatment of malignant lymphoma.


Energy balance and body composition changes. A critical study of three recent publications.


Current metabolic-genetic interrelationship in human atherosclerosis, with therapeutic considerations.


A controlled study of the prophylactic portacaval shunt. A final report.


Hemophilia in the Talmud and rabbinic writings.


Gold from dross: the first century of the urobilinoids.


Prophylactic portacaval shunts.

Prophylactic portacaval shunts.


The use of pharmacologic agents in atherosclerosis therapy.


The relation of microangiopathies to arteriosclerosis, with special reference to diabetes.


The role of thrombogenic factors in atherosclerosis.


Embryological aspects of atherogenesis.

Embryological aspects of atherogenesis.


Wolf-Wito von Wittern, pioneer in ballistocardiography.


Patterns of atherosclerosis of extracranial cerebral arteries.


Studies on a small group of normal test pilots with special reference to quantitative relationships of age with the ULF ballistocardiogram and other cardiovascular measurements.


Vitamin A and the skin.

Vitamin A and the skin.


Diguanides and weight reduction.

Diguanides and weight reduction.


Effect of antihypertensive drugs on the "intrinsic" heart rate. "Intrinsic" heart rate in man was measured by giving simultaneous atropine and propranolol and used to study the direct effect of antihypertensive drugs on the heart. Though the intrinsic and control heart rates decreased significantly following the administration of reserpine, there was no significant difference between high and low doses. No similar effects were observed with debrisoquine sulphate and methyldopa, though in high doses the latter produced a decrease which was of some statistical significance.


Sir Samuel Bedson.

Sir Samuel Bedson.


E. M. Dunlop.

E. M. Dunlop.


E. E. Lewis.

E. E. Lewis.


A study of Fabry's disease. II. Light and electron microscopy.


Metabolic bone disease secondary to renal and intestinal disorders. Metabolic bone disease occurring in renal or intestinal disorders has been reviewed with particular reference to etiological factors. Hyperparathyroidism is seen as a recurring cycle of renal damage-hyperphosphatemia-hypocalcemia-parathyroid stimulation-mobilization of bone calcium and phosphate-renal tubular phosphate rejection. In intestinal cases, the initial stimulus is presumably hypocalcemia. Osteomalacia is seen as resulting from phosphate depletion for the following reasons:1. Experimentally, rickets results from dietary phosphate restriction in rats.2. Such rickets is not prevented by the presence of normally adequate amounts of dietary vitamin D, and may therefore be termed "resistant" in the clinical sense.3. Osteomalacia or rickets in intestinal malabsorption and renal tubular disorders is associated with hypophosphatemia due to excessive fecal or urinary loss.4. Renal tubular rickets has been healed by oral phosphate loading in some studies.5. Acidosis may induce osteomalacic changes, experimentally and clinically (for example, in uretero-sigmoidostomy). Reversal of systemic acidosis with oral bicarbonate has resulted in phosphate retention and a rising serum phosphate in one such case.6. Preliminary data from analysis of full-thickness bone biopsy in two osteomalacic patients shows a significant reduction in calcium and phosphate content.7. Despite the hyperphosphatemia of azotemic renal failure, over-all phosphate depletion may be present in this situation also due to: * Diminished dietary phosphate in low protein diets * Nausea and vomiting * Occasional diarrhea * The use of oral phosphatebinding antacids * Perpetuation of urinary phosphate losses by reduction in proportion of tubular reabsorbed phosphate (secondary hyperparathyroidism) and possibly high filtered load per nephron * Repeated losses of phosphate to bath fluid during dialysis.


Radiological manifestations of drug-induced disease.


Exclusion, superinfection immunity and abortive recombinants in I+ X I+ bacterial crosses.


Models of the nervous system in eighteenth century psychiatry.


Effect of smoking cigarettes on pentagastrin-stimulated gastric secretion in normal persons and patients with peptic ulcer.


Treatment of neonatal jaundice with phenobarbitone.


Control of chronic portal-systemic encephalopathy by lactulose.


An assessment of the validity of the haemolysis test in differentiation of cholera vibrios.


Technique to separate and concentrate cells in metaphase: a modification of direct method for chromosome studies of human bone marrow.


List of members F. D. I.

List of members F. D. I.


The gout encomium of Georg Fleissner, 1594.


Thomas Hodgkin, M. D. (1798-1866): an annotated bibliography.


Professor L. Halpern (1902-1968). In memoriam.


Prizes for yellow fever research in the 1880's.


Molecular structure of RNA polymerase and its complex with DNA.


Freud versus Jung--the mythophobic versus the mythophilic temper in psychotherapy.


The cross-thigh flap in management of open wounds of the lower extremity.


Mental health resources directory.

Mental health resources directory.


Betamethasone in traumatic oral surgery.


Intraoral skin grafts: an aid to alveolar ridge extension.


Primary (non-delayed) cross-leg flaps for early wound coverage.


Robert Gooch (1784-1830), psychiatric midwife.


The significance of class 3 cells in the gastric cytology.


Psychiatric disorders simulating medical emergencies.


Approach to acute intracranial disorders.


Occlusive arterial emergencies.

Occlusive arterial emergencies.


Nephrologic emergencies: acute renal failure.


Nephrologic emergencies--fluids and electrolytes.


Management of the acute manifestations of peptic ulcer.


Platelet function (third of three parts).


Current concepts: intestinal motility.

Current concepts: intestinal motility.


The "neurotic edema" (hereditary angioedema).


Formation, transport, metabolism and excretion of bilirubin.


Reduction of linear blurring in tomography.


Roentgenographic features of the neutrophil dysfunction syndromes.


Television roentgen image subtraction using one camera.


William Hyllis Seward, M. D., 1904-1968.


Robert Edwin Rickenberg, M. D., 1915-1968.


In memoriam. Charles A. Wilkie, D. D. S. (1889-1969).


A new salmonella serotype: S. bilu equals 1,3,(10),19: f,g,t: 1,(2),7.


A convenient procedure for preparing transfer ribonucleic acid from Escherichia coli.


Ultraviolet inactivation of the functions of phenylalanine and lysine transfer RNA's of Escherichia coli.


Studies on ribosomal RNA structure. I. The isolation of crystallizable fragments.


Reversible dissociation of Escherichia coli ribosomes by N-ethylmaleimide.


Replication of bacteriophage M13. I. Sedimentation analysis of crude lysates of M13-infected bacteria.


DNA replication in Mycoplasma laidlawii B.


Protein initiation without folate in Streptococcus faecium.


Interaction of oxidized polyamines with DNA. IV. Effect of DNA composition on the binding.


Single-strand breaks in Escherichia coli DNA caused by treatment with nitrosoguanidine.


The presence of 2-methylthio-N6-(delta-2-isopentenyl) adenosine in serine and phenylalanine transfer RNA's from Escherichia coli.


Role of valine transfer RNA in control of RNA synthesis in Escherichia coli.


Disodium cromoglycate in the control of asthma. A double-blind trial.


Effects of Tris and dimethylsulfoxide on the aminoacylation of Escherichia coli valine transfer RNA by Neurospora crassa phenylalanyl transfer RNA synthetase.


From association to union: professional organization of Asylum Attendants, 1969-1919.


Immunochemical studies on Salmonella serogroup 48. 3. N-acetylneuraminic acid as an immunodominant sugar in S. dahlem.


Fractionation and amino acid sequences of peptides obtained by peptic hydrolysis of baker's yeast cytochrome c.


Infection by bacteriophage P1 and development of host-controlled restriction and modification and of lysogenic immunity. Shigella dysenteriae cells were infected with phage P1 or P1cl. The outcome of superinfection of these cells with phage T1.Sh or T1.Sh(P1) or P1cl was studied as a function of time after the initial infection. Cells undergoing either a lytic response or a lysogenic response to the primary infection develop the ability to specifically restrict T1.Sh between 30 and 45 min. Between 15 and 30 min, the cells seem to develop the ability to produce T1.Sh(P1) after infection by T1.Sh. However, reasons are given for believing that this apparent time difference is consistent with a simultaneous development of the two capacities (restriction and modification) within the cell. This development occurs between 30 and 45 min. Cells infected with P1cl and superinfected 45 or more min later with T1.Sh(P1) can yield both P1cl and T1. Cells infected with P1 become resistant to infection by P1cl within 5 to 10 min. It is argued that this early immunity is not necessarily different in mechanism from true lysogenic immunity.


Temperature-sensitive mutants of respiratory syncytial virus. Four conditional-lethal temperature-sensitive mutants of RS virus were detected among the progeny of 454 plaques derived from virus grown in the presence of 10(-4)m 5-fluorouridine. These mutants were stable (reversion frequency, 10(-5.0) or less and failed to produce plaques at 38 or 39 C. Plaquing efficiency was depressed 100-fold or more at 37 C. Variable suppression of growth at the restrictive temperature of 39 C was observed, ranging from 16-fold to complete suppression. The temperature-sensitive defect of three of the mutants appeared to affect functions which were expressed late in the replicative cycle. One of the mutants produced atypical nonsyncytial plaques.


Induction of P1 prophage and superinfection by bacteriophage T1. Induction of the resident prophage did not permit restricted phage T1 to replicate freely in P1-lysogenic hosts. A few induced cells did become infectible.


Immunocytologic and serologic diagnosis of influenza A2.


Comparison of three serologic methods in the diagnosis of influenza A2. Usefulness of allantoic fluid antigen in the CF test.


Documentation of the alcohol literature.


Studies on the receptors in Ciona intestinalis. 3. A second type of photoreceptor in the tadpole larva of Ciona intestinalis.


Development of the ciliary complex and microtubules in the cells of rat subcommissural organ.


Hering and the scientific establishment.


Artifact emphysema.

Artifact emphysema.


Schizophrenia: diagnosis and prognosis.

Schizophrenia: diagnosis and prognosis.


Histopathological study of lymphocyte transfer in man.


The growth and development of a profession.


Renin secretion in the patient with hypertension. Relationship to intrarenal blood flow distribution.


The distribution of hypertensive changes in small pulmonary vessels.


Age changes in the cross-linking of human collagen.


Radioprotective effect of rat kangaroo haematopoietic cells on x-irradiated mice and Chinese hamsters.


The blue pigment and mucilage of Schizophyllum commune.


The antagonistic action of homopantothenic acid against pantothenic acid.


Endocarditis from Serratia marcescens.

Endocarditis from Serratia marcescens.


Forty years of full-time medicine at University of Chicago. Evaluation and affirmation.


Hereditary galactosemia.

Hereditary galactosemia.


Richard Mead (1673-1754) successor to John Radcliffe.


Dr. Friedrich Reimann.

Dr. Friedrich Reimann.


Red cell mass determination--a common radioisotope procedure.


Fine structure analysis of a chromosome segment in Drosophila melanogaster. Analysis of x-ray-induced lethals.


DNA repair and induction of mutants with altered DNA base composition: studies on Escherichia coli and Bacterium paracoli.


Induction of mutations in bromouracil-containing Salmonella typhimurium exposed to ultraviolet light.


Catherine Gross Duncan (1908-1968).

Catherine Gross Duncan (1908-1968).


Preparation of epidermal imprints of living plant organs by the use of nontoxic mucilage and latex.


Isolation of living endothelial cells by gelatin-film stripping of vascular walls.


A widely applicable technic for direct processing of bone marrow for chromosomes of vertebrates.


Burnishing uncoated grids before mounting ultrathin sections; a means of assuring adhesion.


The use of a xylene spray for restoring compressed paraffin serial sections.


Chronic disseminated histiocytosis X with involvement of the eyelid.


The cephalosporins in ophthalmology.

The cephalosporins in ophthalmology.


Impact of Medicare and Medicaid on resident training and clinical research in opthalmology.


A controlled sevn year study of endocrine and other indices in drug treated chronic schizophrenics.


Special issue in honor of Dr. Harvey Carlyle Pollock, Sr.


Special issue in honor of Dr. Harvey Carlyle Pollock, Sr., Preface.


An appreciation of Dr. Harvey Carlyle Pollock, Sr.--orthodontist, editor, teacher, rancher, gentleman.


A critical appraisal of tongue-thrusting.


Growth studies of the dentition: a review.


The Crozat removable appliance.

The Crozat removable appliance.


Principles of retention and avoidance of posttreatment relapse.


Polymer science in the prevention of air pollution.


Heat resistance of Salmonella in various egg products. The heat-resistance characteristics of Salmonella typhimurium Tm-1, a reference strain in the stationary phase of growth, were determined at several temperatures in the major types of products produced by the egg industry. The time required to kill 90% of the population (D value) at a given temperature in specific egg products was as follows: at 60 C (140 F), D = 0.27 min for whole egg; D = 0.60 min for whole egg plus 10% sucrose; D = 1.0 min for fortified whole egg; D = 0.20 min for egg white (pH 7.3), stabilized with aluminum; D = 0.40 min for egg yolk; D = 4.0 min for egg yolk plus 10% sucrose; D = 5.1 min for egg yolk plus 10% NaCl; D = 1.0 min for scrambled egg mix; at 55 C (131 F), D = 0.55 min for egg white (pH 9.2); D = 1.2 min for egg white (pH 9.2) plus 10% sucrose. The average Z value (number of degrees, either centigrade or fahrenheit, for a thermal destruction time curve to traverse one logarithmic cycle) was 4.6 C (8.3 F) with a range from 4.2 to 5.3 C. Supplementation with 10% sucrose appeared to have a severalfold greater effect on the heat stabilization of egg white proteins than on S. typhimurium Tm-1. This information should be of value in the formulation of heat treatments to insure that all egg products be free of viable salmonellae.


Immunofluorescent identification of Salmonella typhi during a typhoid outbreak. Immunofluorescence and conventional bacteriological methods were compared for their ability to detect Salmonella typhi in 134 fecal specimens from 105 individuals associated with an outbreak of typhoid fever. Smears prepared from untreated fecal material (direct method) and after a preliminary incubation in selenite F broth (delayed method) were tested with an anti-Vi serum conjugated with fluorescein isothiocyanate. The delayed method was more sensitive than the direct method in detecting S. typhi. The delayed method was positive in 40 of 41 patients positive by culture methods, but gave positive or questionable reactions in 11 presumably uninfected individuals. The fluorescent-antibody test employing a Vi conjugate is a satisfactory screening procedure for detecting S. typhi, but all positives must be confirmed bacteriologically.


Interaction of salt, pH, and temperature on the growth and survival of salmonellae in ground pork. The interaction of temperature, pH, and NaCl concentration on the growth and survival of several strains of salmonellae has been determined in broth and ground pork. Growth of 23 strains occurred in broth at 30 C over a wide range of pH-NaCl combinations; at 10 C, growth was limited to only a few combinations. Cultures which would not grow at 10 C because of the pH-NaCl effect survived for long periods, however. In contrast, cultures which would not grow at 30 C remained viable for only a short time. Results in fresh ground pork were in close agreement with the broth studies. Salmonellae would not grow in ground pork stored at 4 C but would grow in pork containing 3.5% salt stored at 10 C. Salmonellae grew competitively with the natural background flora at 10 C even when the salmonellae constituted less than 5% of the initial flora, and the background flora would grow at a lower temperature than the salmonellae. The data show that, whereas decreasing temperatures increase the inhibitory effects of pH and NaCl, they decrease the lethal effects.


Accelerated procedure for Salmonella detection in dried foods and feeds involving only borth cultures and serological reactions. A procedure has been developed in which Salmonella can be detected in dried foods and feeds within 50 hr. This includes preenrichment (18 hr), selective enrichment (24 hr), elective enrichment (6 to 8 hr), and serological testing (2 hr). The procedure is as sensitive as the more time-consuming, traditional (plating, biochemical, and serological) procedure which may require at least 4 to 5 days. The new procedure is rapid and accurate in comparison to traditional procedures. Moreover, it is simple and inexpensive to perform. As described, the procedure does not necessitate the isolation of pure cultures, but this step is performed easily if desired.


Comparison of two procedures for detection of Salmonella in food, feed, and pharmaceutical products. A survey of food, feed, and pharmaceutical products was undertaken to compare an accelerated Salmonella detection procedure by enrichment serology (ES) to the traditional procedure outlined in the Bacteriological Analytical Manual (BAM). Excellent agreement between the two methods was obtained in the results of 689 test samples involving 35 different products. In addition to being more rapid and simpler to perform, the ES procedure was just as accurate and sensitive as the BAM procedure.


Incidence study of spores of Clostridium botulinum in convenience foods. The objective of this study was to gather data on the incidence of Clostridium botulinum spores in selected consumer-convenience food products. The incidence of spores of C. botulinum in 100 samples of each of four categories of commercially available convenience foods was determined. These categories included (i) "boil-in-the-bag" foods, (ii) vacuum-packed foods, (iii) pressurized foods, and (iv) dehydrated and freeze-dried foods. Of the 400 samples analyzed, one was found to contain the spores of C. botulinum. This occurred in vacuum-packed frank-furters and was identified as type B.


Cometabolism and gas chromatography for the sensitive detection of bacteria. Salmonella typhimurium, Escherichia coli, and Staphylococcus aureus were grown in media containing one of several halogenated organic acids which the bacteria metabolized but could not use as a major carbon and energy source for growth. Gas chromatographic techniques were employed to detect the presence of low population densities by analysis for products formed by the microorganisms. By virtue of the presence of the cometabolizable halogenated substrate in the medium, the sensitivity of procedures for detecting the presence of S. typhimurium, E. coli, and S. aureus was increased more than 7,000-, 20,000-, and 1,000-fold, respectively.


Isolation of anaerobic bacteria from human gingiva and mouse cecum by means of a simplified glove box procedure. An anaerobic glove box constructed of clear flexible vinyl plastic is described. It is sufficiently inexpensive and simple in operation to be used not only in research but also in a clinical laboratory by technicians without special training. Conventional bacteriological techniques may be used inside the glove box for culturing and transferring anaerobic bacteria. The box may be heated to 37 C and thus serve as an anaerobic incubator as well, permitting inspection of cultures at any time. Media may be prepared and agar plates may be poured on the laboratory bench in the conventional manner. An overlay of trace amounts of palladium black catalyst over plated agar media reduces the medium to an oxidation-reduction (O-R) potential of - 300 mv within 2 days after introduction into the glove box. In spite of its greater simplicity, the system matched or excelled the roll tube method with respect to all parameters tested, including O-R potential obtainable in the media, O(2) concentration in the gas phase, and efficiency in isolating anaerobic bacteria from the mouse cecum. Comparative studies indicate that the conventional anaerobic jar method was inadequate for the isolation of strict anaerobes from human gingival specimens and from the mouse cecum. This was due to the exposure of specimens and media to air during plating on the open laboratory bench. Anaerobic jars were adequate for maintaining the proper conditions for growth of anaerobic bacteria once these had been established in the glove box.


Fecal microflora in health persons in a preindustrial region. Procedures for quantitating the fecal microflora of man were described. Special attention was given to criteria for characterizing the culturable aerobic, Micro-aerophilic, and anaerobic bacteria. Three groups of healthy persons were studied: wholly breast-fed infants (2 to 4 month-olds), weanlings (1 to 2 year-olds), and adults. In breast-fed children, bifidobacteria predominate and outnumber by one or several logs all other culturable bacteria. The fecal flora of wholly breast-fed infants is "simpler" and more numerous [10(11) to 10(12) per g (wet weight) of feces than that of the adult 10(2) to 10(11) per g]. In the adult, gram-negative anaerobic bacilli (bacteroides) outnumber all other groups by a factor of 1 log or more. Weanlings receiving an adult-type diet, but still breast-fed, showed a flora intermediate between that of the wholly breast-fed infant and that of the adult, but more similar to the latter. Anaerobes always constitute the predominant component of the culturable flora of children and adults and are always found in large numbers under conditions of health. The aerobes are significantly less numerous, and vary widely in their number and in the frequency with which they appear in feces.


Identification of group A streptococci by direct fluorometry. A simple direct fluorometric method for rapid identification of group A streptococci is described. The method permits the detection of the organism in mixed cultures without the aid of a microscope and is amenable to automated processing of specimens. Experience with the indirect fluorometric method revealed that nontrypsinized cells from a 10-fold dilution of overnight broth cultures could be stained with uniform brilliance with fluorescent antibody (1:15 dilution) and that fluorescent antibody dissociated from such cells at 55 C for 20 min gave serologically specific fluorometric values. With this information, it was possible to develop a simpler fluorometric test which gave results comparable to those obtained by conventional cultural-precipitin grouping techniques. In the direct test described, cultures from throat swabs were incubated overnight, and cells from a 10-fold dilution were stained with specific fluorescent antibody (1:50 dilution) and then rinsed. The stained specimens were transferred to a continuous-filter paper strip (Whatman 3 MM) and read serially in a Turner 110 fluorometer with Corning 5840 and Wratten 2A filters in place. The reagents used required careful standardization and testing to assure that fluorometric readings above a specified value would be indicative of the presence of group A streptococci.


Evaluation of plating media for the determination of viable microorganisms in dental plaque. Four agar media were evaluated to determine which one might be most suitable for the determination of the predominant number of viable microorganisms in dental plaque. The highest plate counts and uniformly larger microbial colonies were consistently obtained with Brain Heart Infusion Agar.


Glutaraldehyde inactivation of virus in tissue. High concentrations of influenza virus and T3 coliphage were inoculated into mouse tissue blocks. Exposure of the inoculated tissue blocks to 5% alkaline glutaraldehyde resulted in rapid inactivation of both viral agents.


The purification and physicochemical properties of a lytic enzyme induced by coliphage N20F'.


Partial purification of mitochondrial and supernatant DNA polymerase from Saccharomyces cerevisiae.


Two types of pyruvate kinase in Escherichia coli K12.


Functional aspects of localization in the cat's tectum opticum.


Distribution of the dorsal root fibers in the cat. An experimental study with the Nauta method.


Nature of in-vitro plasma coagulation by gram negative bacteria.


Hypnosis as a method for investigating unconscious thought processes. A review of research.


Fluoride and bone.

Fluoride and bone.


The biochemical characterization of two mutant arginyl transfer ribonucleic acid synthetases from Escherichia coli K-12.


Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase.


Enzymatic synthesis of deoxyribonucleic acid. XXIX. Hydrolysis of deoxyribonucleic acid from the 5' terminus by an exonuclease function of deoxyribonucleic acid polymerase.


Enzymatic synthesis of deoxyribonucleic acid. XXXI. Binding of deoxyribonucleic acid to deoxyribonucleic acid polymerase.


Coordinate control of ribonucleic acid synthesis during uracil deprivation.


Carbon monoxide-binding pigments of Mycobacterium phlei and Escherichia coli.


Freeze-etching observations on tobacco leaves infected with tobacco mosaic virus.


Diabetes with vitiligo.

Diabetes with vitiligo.


Stereotaxic (three dimensional) neurosurgery.


Covalent repair of molecular recombinants in the ligase-negative amber mutant of T4 bacteriophage.


Role of lysozyme in the biological activity of bacteriophage ghosts.


Antibacterial action of decamethylenebis-(4-amino-5,6,7,8-tetrahydroquinaldinium) acetate against Escherichia coli.


Rapid gas chromatographic analysis of microbial volatile metabolites.


"Some reflections on genius".

"Some reflections on genius".


Progress report: the battle of the bottle.


II. Does occur the multiplication of shigellae phagocytized in the cells?


II. An attempt of Shigella sonnei bacteriological negativeness with neomycin.


Problems of persistance of Plectridium tetani in the portal of entry in patients with tetanus.


Disinfection in veterinary practice.

Disinfection in veterinary practice.


Antecedents of the Semmelweisian concept in Hungary's medical literature.


Role of biological membranes in some skeletal reactions.


Significance of nuclear immunofluorescent patterns.


Azathioprine: a controlled, double-blind trial in rheumatoid arthritis.


The trials of the medical student.

The trials of the medical student.


Robert Adair.

Robert Adair.


On doing research in schizophrenia.

On doing research in schizophrenia.


Overview of the causes and prevention of violence.


Effects of diphenylhydantoin on disruptive behavior. Study of male delinquents.


Types of hopelessness in psychopathological process.


Experimental hypertrophic neuropathy. Pathogenesis of onion-bulb formations produced by repeated tourniquet applications.


Early activities for collegiate nursing education in Arizona.


The first decade.

The first decade.


Reactions of the graft versus host (GVH) type.


Pathologic changes in canine renal allografts with prolonged function.


Further studies on artificial antigens and immunity to mouse typhoid. II. The ability of galactans containing various acyl groups to immunise mice against mouse typhoid.


Susceptibility of mild thermal and of ionizing radiation damage to the same recovery mechanisms in Escherichia coli.


Unbiased participation of T4 phage DNA strands in replication.


Bupivacaine: a clinical assessment in lumbar extradural block.


Fatal haemorrhage from the innominate artery after tracheostomy. A case report.


Anti-haemorrhagic effect of ethamsylate under hypotensive anaesthesia.


Azathioprine in active chronic iridocyclitis. A double-blind controlled trial.


Plastic surgery for pigmented hairy naevus of the eyelids. By excision and masquerade skin graft.


One hundred years of health: New York City, 1866-1966.


Paul F. Schilder, M.D., and his contributions to adolescent psychiatry.


Medical numismatic notes. II. The Montrose Lunatic Hospital.


Boswell's gonorrhea.

Boswell's gonorrhea.


Virus-specific proteins in Escherichia coli infected with the RNA bacteriophage MS2.


Forensic medicine.

Forensic medicine.


Antilymphocyte serum: present status.

Antilymphocyte serum: present status.


On the regulation of hemopoietic spleen colonies produced by embryonic and adult cells.


Proteinuria in human renal allografts.

Proteinuria in human renal allografts.


Medicare and the hospital revolution.

Medicare and the hospital revolution.


On the presentation of the Friedenwald award in ophthalmology to Dr. Alfred J. Coulombre.


The use of a progestational agent in the immediate postpartum period.


Atypical mitochondria in the ellipsoid of the photoreceptor cells of vertebrate retinas.


A comparative study of some indicator dilution techniques for blood volume determination in normals and in cases of bilharzial hepatosplenomegaly.


The effect of epithelial remnant and whole organ grafts of thymus on the recovery of thymectomized irradiated mice. Work has been presented which suggests that thymus epithelial reticular cells are not effective in restoring the microscopic morphology of lymphoid tissues and their immunologic capacities. They function in recruiting precursors of thymus lymphocytes from the host animals to produce an organ which, after it becomes architecturally normal, can reconstitute the defective host. Intact thymus grafts in situ from 10-14 days, but not for shorter periods of time, have been shown to result in a return toward normal of these two parameters. Evidence is offered to show that few dividing cellular components in the lymphoid tissue originate from the thymus remnant grafts, and that a minor cellular component is contributed by the intact grafts. These data support the concept that the structural and functional development of the lymphatic tissue in thymectomized animals is dependent on thymus lymphoid cells and/or their products, and that the epithelial-reticular cells do not have a direct action in peripheral lymphoid reconstitution.


Cells involved in the immune response. VII. The demonstration, using allotypic markers, of antibody formation by irradiation-resistant cells of irradiated rabbits injected with normal allogeneic bone marrow cells and sheep erythrocytes. Bone marrow cells obtained from rabbits of one allotype were injected into irradiated rabbits of a different allotype. The recipients were also injected with sheep red blood cells, and their spleen cells were tested for plaque-forming capacity 7 days later. Spleen cells of all recipients gave large numbers of plaques as did spleen cells incubated with antiserum, directed toward donor allotype. However, incubation of the recipient spleen cells with antiserum directed toward recipient allotype completely suppressed plaque formation. These results demonstrate that antibody-formation in irradiated recipients of transferred lymphoid cells is a property of the recipient animal and that the antibody-forming cell is relatively irradiation-resistant. It was also demonstrated that only viable normal bone marrow cells are capable of transferring antibody-forming capacity to irradiated recipient rabbits. Neither sonicates nor heat-killed preparations of normal rabbit bone marrow cells possessed this capacity.


Contribution to the problem of the occurrence of natural foci of Q fever.


The effects of methylphenidate (Ritalin) on the motor skills and behavior of children with learning problems.


Intravascular gas emboli. A selected literature critique.


Water Pollution Control Federation. Federation membership.


The effect of various drugs on the uptake and metabolism of tyramine-H3 in the rat heart.


Contractile activity of human glomeruli in culture.


A mutant of Escherichia coli K12 exhibiting varying ultraviolet sensitivities depending on the temperature of incubation after irradiation.


Current literature survey of photosynthesis--XVII.


The effects of hypopotassemia on the bioelectric activity of skeletal muscles in the chronic cor pulmonale syndrome.


Remarks on sex chromatin determination in breast cancer.


The value of the fluorescent treponemal antibody inhibition test for the diagnosis of syphilis.


The assay of pepsin.

The assay of pepsin.


Chronic skin graft rejection in the Urodela. II. A comparative study of xenograft rejection.


The effect of skin allografts on the reticuloendothelial system.


Prolonged cancine renal allograft survival after pretreatment with solubilized antigen.


The importance of target size in the destruction of skin grafts with non-H-2 incompatibility.


Allogeneic canine bone marrow transplantation following cyclophosphamide.


Estimates of the number of loci contributing to the histoincompatibility between C57BL-6 and BALB-c strains of mice.


Resistance to skin grafts induced by cell extracts in congenic strains of mice differing from the donor at various non-H-2 loci.


Effect of parental strain thymus grafts in neonatally thymectomized F1 hybrids.


Leukocyte antigens in renal transplantation. IV. The effect of blood transfusions on leukocyte typing by lymphocytotoxicity.


Examination of an inbred guinea pig strain for weak histocompatibility differences.


Endothelial cell--leukocyte bridges in skin allografts.


Kidney transplantation in siblings. Is immunosuppressive treatment invariably needed?


The effect of actinomycin D on the synthesis of influenza virus-specific RNA.


Defective bactericidal activity of monocytes in fatal granulomatous disease.


Immunochemical studies on Salmonella typhi. V. The nucleic acids content of Cetavlon fractions from typhoid bacilli S and R forms.


Calculation of precision in isotope dilution experiments.


Obituary. Sir Henry Hallett Dale, O.M., F.R.S.


Obituary. Corneille Heymans.

Obituary. Corneille Heymans.


Mode of action of clomiphene. I. Inhibitory effect of clomiphene citrate on the formation of progesterone from acetate-1-14C by human corpus luteum slices in vitro.


Skin-fold thickness in normal subjects and in patients with acromegaly and Cushing's syndrome.


Radioimmunoassay of insulin in plasma and urine in obese subjects and in diabetic patients.


Effect of dexamethasone on calcium metabolism and 47Ca kinetics in normal subjects.


Studies on the human placenta. II. Gross morphology of the foetal structures in the young placenta.


Oral contraception using a sequential method. A clinical investigation.


Clinical and seminal findings in men with sperm antibodies.


The value of plastic operations on the fallopian tubes in the treatment of female infertility. A clinical and radiological study.


Clinical test of Kebuzon versus phenylbutazone in rheumatoid arthritis.


Survival of Clostridium botulinum spores in foods treated by heat, ionizing radiation, or related procedures.


Two types of synaptic bulb in snake and frog spinal cord: the effect of fixation.


Real hearts.

Real hearts.


Immunopathological studies on experimental arthritis-lesions in synovial tissue during endotoxemia.


Prochlorperazine--a review of the literature since 1956.


Laboratory changes with chlordiazepoxide and thioridazine, alone and combined.


A blind comparative trial of nortriptyline and isocarboxazid in depressed outpatients.


Sedatives and hypnotics in psychiatry.

Sedatives and hypnotics in psychiatry.


Psychotherapy, what, how and why?

Psychotherapy, what, how and why?


Combined orthodontic and prosthetic treatment of a Class II, Division 2 case with missing upper lateral incisors.


Sociometric study of the sex offender.

Sociometric study of the sex offender.


Validity of clinical judgments based on human figure drawings.


DAP: back against the wall?

DAP: back against the wall?


Self-disclosure and interpersonal functioning.


Bibliography of the literature on antibiotics published in foreign countries. 74.


Extradural spinal cyst, mechanism of spinal cord compression.


The other Max Nordau, M.D. (1849-1923).

The other Max Nordau, M.D. (1849-1923).


Portrait of a leader: Lavinia Lloyd Dock.


Baccalaureate and Masters Degree Programs in nursing accredited by NLN, 1969-70.


Synthetic cardenolides.

Synthetic cardenolides.


Skin blood flow in obliterative arterial disease of the leg. Effect of vascular reconstruction examined with xenon and iodine antipyrine clearance and skin temperature measurements.


Mass spectrometry of carbohydrate derivatives.


Feathers and patterns.

Feathers and patterns.


The vitelline membrane and cortical particles in sea urchin eggs and their function in maturation and fertilization.


The initiation of organized development in plants.


Adolf Hitler. His life and his illness.

Adolf Hitler. His life and his illness.


The stimulus to hypertrophic growth.

The stimulus to hypertrophic growth.


The effects of hypoxia on certain aspects of visual performance.


A comparative analysis of macro- and microsurigal neurorrhaphy technics.


Diagnostic considerations in anomalous innominate arteries.


Clinical evaluation of estrogen-androgen combination in advanced breast cancer.


Alzheimer's disease.

Alzheimer's disease.


The uvea.

The uvea.


Importance of the osmolarity of the incubation medium on amino acid incorporation into protein by isolated rat liver mitochondria. 1. Incorporation of [(14)C]leucine into protein by isolated rat liver mitochondria was examined by using incubation media similar to those used by Sandell, Löw & Decken (1967) (medium A) and Roodyn, Reis & Work (1961) (medium B). The incorporation process was found to be almost completely inhibited in medium A. 2. By decreasing the amount of sucrose and omitting tris-hydrochloric acid from medium A, incorporation proceeded at a rate higher than that found in medium B. It was found that the inhibitory action of medium A was due to its high osmolarity. 3. Oxidative phosphorylation and RNA synthesis by the isolated mitochondria proceeded at the same rate in media essentially the same as media A and B. 4. There was a partial inhibitory action of medium A on leucine uptake by the mitochondria and also on the formation of leucyl-transfer-RNA. The major block of inhibition by the hyperosmolarity of medium A seemed to be located at a later step of protein synthesis involving mitochondrial ribosomes. 5. Protein synthesis by Escherichia coli B was only slightly inhibited, if at all, in hyperosmotic media in which protein synthesis by isolated mitochondria was completely stopped.


Acrodermatitis enteropathica: Report of 2 cases treated with diiodohyroxyquin.


The substrate specificity of 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase.


Calcified tissues in the earliest vertebrates.


First recorded quintuplets in Africa.

First recorded quintuplets in Africa.


Computer-aided stereotach.

Computer-aided stereotach.


The measurement of perceived size and distance.


Electrophysiology of vibratory perception.


Temporal features of input as crucial factors in vision.


[Prof. Dr. Dr. G. Steinhardt 65 years old].


Tropical sprue.

Tropical sprue.


Streptococcus pyogenes infection in a maternity hospital.


Atypical responses to the glucose tolerance test in normal subjects.


Some aspects of carbohydrate metabolism in pregnancy with special reference to the energy metabolism and hormonal status of the infant of the diabetic woman and the diabetogenic effect of pregnancy.


Intracerebral autografts of mouse tail skin.


Effect of altitude on the microbiota of man.


Histoogical studies on weaned pigs suffering from diarrhoea and oedema disease produced by oral inoculation of Escherichia coli.


John Rupert Squire, 20 August 1915--6 January 1966.


Craniostenosis in one dizygotic twin.

Craniostenosis in one dizygotic twin.


Fat embolism in the microcirculation: an in-vivo study.


Ultrastructure of the wall of small arteries in early experimental rat hypertension.


Colladon of Geneva, a precursor of Mendel?


Auto-phagocytosis in mouse typhoid.

Auto-phagocytosis in mouse typhoid.


The evolution of orthopaedic surgery in north-west England (Jones R).


The history of anaesthesia in Edinburgh. 2.


Cardiopulmonary resuscitation: the anesthesiologist's role.


Thrombophlebitis and pulmonary embolism.


Emergency treatment of severe bacterial infection.


Pheochromocytoma: observations on mechanism of carbohydrate intolerance and abnormalities associated with development of Goldblatt kidney following removal of tumor.


Serum insulin and growth hormone in lipoatrophic diabetes.


Evaluation of three acute tests of hypothalamic-pituitary-adrenal function.


Metabolic response to simultaneous infusion of epinephrine and propranolol in obese adolescents.


Effect of allopurinol on urinary ammonia excretion in patients with gout.


Temperature sensitive mutations affecting RNA synthesis in Escherichia coli.


Childhood malnutrition in Iran.

Childhood malnutrition in Iran.


Tularemia epidemic: Vermont, 1968. Forty-seven cases linked to contact with muskrats.


Prostaglandins and cardiovascular diseases.


Low caloric intakes of Jamaican adults.

Low caloric intakes of Jamaican adults.


Cyclic behavior of amino acid transport.


Anticonvulsant therapy and serum folate.


Absorption and utilization of glucose by rats fed once daily or ad libitum.


Skin changes in essential fatty acid deficiency in mice.


Thiamine and oxythiamine metabolism.

Thiamine and oxythiamine metabolism.


Report on a study of cerebral biopsy in general paretics.


Neurology in Bombay in the 1840s.

Neurology in Bombay in the 1840s.


SODAT House: Doctor "Pat" started screaming and hasn't stopped yet.


South American snuffs. Anthropological survey of the use of South American snuffs.


An overview of ethnopharmacology. Historical survey.


ne hundred years young.

ne hundred years young.


Statement on heart transplantation adopted by the House of Delegates of the American Medical Association at Miami, Florida, December 4, 1968 and by the House of Delegates of the R.I. Medical Society at Providence, R.I., April 23, 1969.


Bacterial growth rates above 90 degrees C in Yellowstone hot springs. Growth rates of bacteria living in boiling springs have been measured by determining rate of increase in cell numbers on microscope slides immersed in the springs. Distinction between growth and passive attachment was made with ultraviolet radiation. In all cases, slides irradiated at intervals had significantly fewer bacteria than controls. Estimated generation times ranged from 2 to 7 hours, values which are comparable to those of aquatic bacteria living in less extreme environments.


Escherichia coli: strains that excrete an inhibitor of colicin B. Mutants exb of Escherichia coli are insensitive to colicin B and excrete into their culture media a material that inhibits colicin activity and that appears to be the bacterial lipopolysaccharide.


The Italian plastic surgery of the XIV century.


Thoracic duct lymph: its significance in dialysis and immunology.


Physiological studies on Clostridium botulinum, type F. Progress report, December 1, 1965-November 30, 1966. ORO-3347-3.


Compendium of human responses to the aerospace envirnment. 9. Sound and noise. NASA CR-1205 (2).


Compendium of human responses to the aerospace environment. 8. Vibration. NASA CR-1205 (2).


Judge David L. Bazelon.

Judge David L. Bazelon.


Programed learning, teaching machines, and dyslexia.


The role of genetics in the etiology of the schizophrenias.


A biographical sketch of Antoine Ferrein.


A biographical sketch of Dr. Landa.

A biographical sketch of Dr. Landa.


Cornea and sclera.

Cornea and sclera.


Keratoplasty and trachoma in the southwestern American Indian.


Helen Hsiu-hsiang Chi, MD.

Helen Hsiu-hsiang Chi, MD.


Studies on tetanus toxin and toxoid. 3. Sedimentation of toxin and derivatives obtained by sulphite and aldehyde treatments.


A modified technique for edentulous flat ridge lower cases.


Royal Army Dental Corps Museum.

Royal Army Dental Corps Museum.


Obituary.

Obituary.


Methotrexate and the liver.

Methotrexate and the liver.


The magnetic cure.

The magnetic cure.


Nosocomial infections.

Nosocomial infections.


Presentation of the seventh Ferdinand C. Valentine medal and award to Terence Millin.


Some Irish urology: ancient and modern. The seventh Ferdinand C. Valentine memorial lecture.


Psychiatric emergencies in a general hospital outpatient department. Some data and a review of the literature.


Psychodynamic theories of the "survivor syndrome.


The history of psychiatry in South Africa.


Cleavage of canavaninosuccinic acid by human liver to form guanidinosuccinic acid, a substance found in the urine of uremic patients.


Speech and its relation to dentistry. 3. The effects on speech of variations in the design of dentures.


The cellular distribution of albumin in normal rat liver demonstrated by immunofluorescent staining.


The treatment of functional disorders of the masticatory system by the use of stainless-steel plates.


From pyorrhoea to periodontics.

From pyorrhoea to periodontics.


A method of studying dental plaque morphology.


Etiology of congenital heart disease.

Etiology of congenital heart disease.


Evidence for an enol intermediate in the enzymatic conversion of alpha,beta-dihydroxyisovalerate to alpha-ketoisovalerate.


Formation of 1,4-diaminobutane and of spermidine by an ornithine auxotroph of Escherichia coli grown on limiting ornithine or arginine.


Synovectomy and débridement of the knee in rheumatoid arthritis. I. Historical review.


The purification and characterization of O-acetylserine sulfhydrylase-A from Salmonella typhimurium.


The isolation of envelopes of Escherichia coli spheroplasts in the zonal ultracentrifuge.


Sacral agenesis.

Sacral agenesis.


Discitis (closed space infection) following removal of lumbar inervertebral disc.


Temperature changes associated with various dental cutting procedures.


The value of urinary beta-glucuronidase activity in the assessment of urinary tract infections.


A study of the cell envelope of the halobacteria.


Growth and pigmentation of Micrococcus radiodurans.


Editorial: Commencement-of what?

Editorial: Commencement-of what?


Characterization of leptospires according to fatty acid requirements.


The mesiodens in the Alaskan eskimo.

The mesiodens in the Alaskan eskimo.


The physical demonstration and characterization of extrachromosomal elements.


Phenotypic masking and drug dependence in streptomycin-dependent and paromomycin-dependent strains of Escherichia coli.


Some aspects of replication of R factors.


Stannous hexafluorozirconate and enamel solubility.


Hereditary hypophosphatemia.

Hereditary hypophosphatemia.


Practical significance of extrachromosal elements.


Tissue response to cortisone containing and cortisone free calcium hydroxide.


Use of fluorescent antibodies for the identification of Streptococci. II. Determination of Streptococcus agalactiae (group B).


Recombination of R factors with Col factors and chromosomal genes in Escherichia coli.


Endodontics--patient performed.

Endodontics--patient performed.


A few remarks on the preparation of tetanus toxin and toxoid on casein hydrolysate medium.


Shigella sonnei mutants rapidly fermenting lactose. II. Influence on fermentation of lactose and salicin.


From the history of Russian-French scientific relationships. Pasteur and Russian doctors.


Rare historical records of Vicksburg Medical School go to SBH library.


Association of American Medical Colleges Proceedings for 1968. Institutional membership meetings.


Isolation of lymphogranuloma venereum agent in the Sudan.


The treatment of urinary schistosomiasis with Ambilhar in Mosul.


Computer method for peak area estimation of indicator dilution curves.


The effects of 6-chloro-8-aza-9-cyclopentylpurine on nucleic acid and protein synthesis in Escherichia coli. I. In vivo studies.


Clinico-pathological conference. Gastrointestinal bleeding.


The effects of 6-chloro-8-aza-9-cyclopentylpurine on nucleic acid and protein synthesis in Escherichia coli. II. In vitro studies.


Fatty acid-conjugated polyamines that alter cell permeability and active transport properties of Escherichia coli.


Analysis of photoenzymatic repair of UV lesions in DNA by single light flashes. II. In vivo studies with Escherichia coli cells and bacteriophage.


Do dark repair mechanisms for UV-induced primary damage affect spontaneous mutation?


Peroxide effects on survival and mutation induction in ultraviolet light exposed and photoreactivated bacteria.


History of pernicious anemia (PA).

History of pernicious anemia (PA).


Mutagenic activity of streptozotocin.

Mutagenic activity of streptozotocin.


Medical pilgrimage leads home (Chai Ho Ahn).


An historical vignette in forensic psychiatry.


Protein synthesis by a mutant of phage S13 blocked in DNA synthesis.


Stanley Cobb, M.D. 1887-1968.

Stanley Cobb, M.D. 1887-1968.


A specific surface antigen induced by poxvirus.


Meprobamate-benactyzine (Deprol) and placebo in two depressed outpatient populations.


Tobacco etch virus cylindrical inclusions: antigenically unrelated to the causal virus.


Toward a concept of comprehensive medicine.


Mutation in gal U gene of E. coli blocks phage P1 infection.


Proved procedures in crown and bridgework.


General principles of chemotherapy of mental illness.


Loss of host-controlled restriction and modification of phage lambda in Escherichia coli K12 previously infected with UV-irradiated coli-phage dT3.


Production of serum-blocking material by mutants of the left arm of the lambda chromosome.


New mutations in the S cistron of bacteriophage lambda affecting host cell lysis.


Further studies on the physiology and pathophysiology of the tuning of the central nervous system.


Syphilis and the eye.

Syphilis and the eye.


Studies on the influence of x radiation on the tooth primordia in the golden hamster (Mesocricetus auratus) in early stages of development.


Experimental mycotic encephalitis.

Experimental mycotic encephalitis.


HIBAC--key advisor to Medicare.

HIBAC--key advisor to Medicare.


An outbreak of Esch. coli 0119 K69 gastroenteritis in a low birth weight baby unit in Ceylon.


Autoimmune reactions and malignant changes in germ-free New Zealand Black mice.


Perpetuation of autoimmune thyroiditis and production of secondary renal lesions following periodic injections of aqueous preparations of altered thyroglobulin.


Atypical myocardial infarction in a case of pemphigus in the light of immunofluorescence studies.


Microphotometry in immunofluorescence.

Microphotometry in immunofluorescence.


Amine synthesis in various brain regions with caudate or septal lesions.


Short-term study of the effect of human parietal cell antibody on the secretion of hydrochloric acid in rats.


Plasmolysis of Escherichia coli B-r with sucrose. Escherichia coli B/r cells were plasmolyzed in sucrose solutions and observed under phase contrast. The prevalence of plasmolysis under various conditions was noted, and the degree of plasmolysis was categorized as slight, extensive, or severe. The presence of ions reduced the prevalence of plasmolysis. Survival curves showed that extensive plasmolysis was not lethal to colony-forming ability.


Mode of action of pesticin.

Mode of action of pesticin. The mode of action of pesticin, a bacteriocin produced by many strains of Pasturella pestis, was studied. Pesticin action on macromolecular synthesis of a sensitive strain of Escherichia coli, strain phi, was found to have features similar to those of colicin E2-317 acting on the same strain. After exposure to pesticin, deoxyribonucleic acid synthesis was arrested and ribonucleic acid was degraded, but little effect was observed on protein synthesis. Pesticin, like colicin E2-317, induced lysogenic E. coli phi (P1), but, unlike the colicin, was active in the presence of dinitrophenol. Trypsin was found to reverse pesticin action up to 15 min after its addition at 40 C to E. coli phi. Pesticin action was studied on three sensitive bacterial strains, P. pestis 2C, P. pseudotuberculosis, and E. coli strain phi, which vary widely in their optimal growth temperature. P. pestis grows best at 29 C, P. pseudotuberculosis at 37 C, and E. coli phi at 40 C. It was found that pesticin action on all three strains was optimal at 40 C. Whereas the titer of pesticin was the same on all three strains when determined on agar, E. coli phi was the most sensitive to pesticin action in broth. No action of pesticin in broth on P. pseudotuberculosis was observed unless Ca ions were added. The effect was not immediate; that is, the cells had to be grown in a medium containing Ca(++) before they displayed sensitivity to pesticin.


Endotoxin-free biologically active component of Escherichia coli. The proteinaceous component of gram-negative bacteria, which has been termed "protodyne," enhances nonspecific host resistance while eliciting a slight pyrogenic response equivalent to 0.2% that of a typical endotoxin. Since this material still contains small amounts of carbohydrate and lipid, it was imperative to establish that its biological activities are not the result of endotoxin contamination. Evidence that the protective activity of protodyne does not result from endotoxin contamination has now been obtained by an evaluation of the Pronase digestion products of this substance. These digestion products were found to be nonpyrogenic and to contain no measurable amount of 2-keto-3-deoxyoctonate, an essential component of bacterial lipopolysaccharides.


Serological studies of Clostridium botulinum type E and related organisms. II. Serology of spores. Pure spore antigens for the immunization of rabbits were prepared by enzymic digestion of vegetative components and separation of the cleaned spores in polyethylene glycol. Spore antisera were prepared to strains representative of toxigenic Clostridium botulinum type E; nontoxigenic boticin E-producing variants; nontoxigenic nonproducers of boticin E; nontoxigenic "atypical" strains, which differ somewhat from C. botulinum type E in their physiology; C. botulinum types A and B; and C. bifermentans. They were tested against these and additional strains representative of the above groups, other types of C. botulinum, and other Clostridium species. There was no evidence of agglutination of flagellar or somatic antigens of vegetative cells by these antisera. Agglutination and agglutinin absorption tests showed common antigens among toxigenic type E strains and nontoxigenic variants, both producers and nonproducers of boticin E. Some nontoxigenic "atypical" strains varied in their ability to be agglutinated by type E antisera, and others did not agglutinate at all. Of those atypical strains that were not agglutinated, one was agglutinated by C. bifermentans antiserum. Antisera prepared against C. botulinum types A and B and C. bifermentans did not agglutinate the spores of type E or its variants nor share antigens common to each other. Similarly, antisera to type E, its nontoxigenic variants, and nontoxigenic atypical strains did not agglutinate other C. botulinum types or any other Clostridium species investigated.


Relation of the segregative origin of chromosome replication to the origin of replication after amino acid starvation. Cultures of Escherichia coli 15T(-) and K-12 were labeled with (3)H-thymine before, during, and after amino acid starvation. The number of labeled segregating units was measured by autoradiography of microcolonies derived from the labeled cells. In both strains, labels inserted before starvation and during starvation appeared to segregate as if incorporated into the same polynucleotide strands. However, labels inserted during and after starvation segregated as if incorporated into different polynucleotide strands. In view of previous data, it was concluded that replication after amino acid starvation originates from the region of the chromosome which serves as the origin for replication during normal growth and division.


Genetic analysis of the maltose A region in Escherichia coli. The genetic map of the maltose A locus of Escherichia coli contains at least three closely linked genes, malT, malP, and malQ. The order of these genes is established by deletion mapping. MalP and malQ, the presumed structural genes for maltodextrin phosphorylase and amylomaltase, belong to the same operon. MalT may be a regulator gene involved in the positive control of this operon.


Isolation and amino acid composition of ribosomal proteins from Bacillus stearothermophilus. Twelve of the proteins from the 30S ribosome of Bacillus stearothermophilus were isolated by preparative disc electrophoresis. Amino acid analyses of these proteins showed them to be different from each other. The gross amino acid composition of 30S ribosomal protein from B. stearothermophilus and Escherichia coli are virtually identical. A number of the proteins of B. stearothermophilus had electrophoretic mobilities similar or identical to 30S ribosomal proteins of E. coli. However, there was little similarity between the two organisms in amino acid composition of individual proteins. There were no unusual chemical features of the B. stearothermophilus proteins which could explain the relative thermal stability of this organism's ribosomes.


Chloramphenicol and the stimulation of ribonucleic acid synthesis in Escherichia coli. Data have been obtained which imply that chloramphenicol stimulation of ribonucleic acid (RNA) synthesis is a result of the accumulation of aminoacyl transfer RNA (tRNA) molecules. The data also support the hypothesis that chloramphenicol exerts an additional effect upon the stimulation of RNA synthesis. This effect may be at the level of the ribosome or the aminoacyl tRNA, or of both. It is this effect combined with the presence of aminoacyl tRNA that results in stimulation by chloramphenicol of RNA synthesis.


Correlation between the rate of ribonucleic acid synthesis and the level of valyl transfer ribonucleic acid in mutants of Escherichia coli. By use of a mutant of Escherichia coli with a partially thermolabile transfer ribonucleic acid (tRNA) synthase, it was possible to regulate the rate of RNA synthesis over a 10-fold range. The addition of chloramphenicol to cultures kept at the nonpermissive temperature stimulated RNA synthesis. The longer the culture was kept at the nonpermissive temperature prior to addition of chloramphenicol, the lower was the resulting rate of RNA synthesis. The decrease in the rate of incorporation of labeled uracil into RNA was correlated with the decrease in the level of valyl tRNA. Additional experiments provided evidence which may be interpreted as indicating that valyl tRNA does not, by itself, react with the RNA-forming system.


Suppression of proline requirement of proA and proAB deletion mutants in Salmonella typhimurium by mutation to arginine requirement. Eleven variants able to grow without proline (provided arginine was absent) were obtained by spontaneous mutation from Salmonella typhimurium LT7 proA and proAB deletion mutants. Suppression resulted from mutation at argG, which specifies N(alpha)-acetylornithine delta-transaminase. In the absence of exogenous arginine, deficiency of this enzyme would cause derepression of the arginine pathway and accumulation of N-acetylglutamic gamma-semialdehyde. N-acetylglutamic gamma-semialdehyde, if deacetylated, would produce glutamic gamma-semialdehyde, the proline precursor whose synthesis from glutamate is blocked in proA and proAB mutants. All of the mutants grew only slowly (some very slowly) if not supplied with arginine. Sonic-treated preparations of eight mutants had no measurable acetylornithine delta-transaminase activity, but those of the three mutants least dependent on arginine had 0.11, 0.28, and 1.48 of wild-type activity; presumably, their enzymes have low specific activity, at least in vivo. Phage P22 cotransduced argG and strA. Genetic analysis showed that the minor degree of arginine dependence of the mutant with greater than wild-type in vitro enzyme activity was a characteristic of its argG allele, not the result of modification of the argG phenotype by mutation elsewhere.


Chromosome transfer from F-lac+ strains of Escherichia coli K-12 mutant at recA, recB, or recC. The frequency of chromosome transfer from various recombination-deficient F-lac(+) donor strains was estimated by standardizing the yield of conjugants receiving a male chromosomal marker against the level of episome transfer in the mating mixture. The efficiency of chromosome transfer from newly formed F-lac(+) cells carrying recB21 or recC22 was more than 50% of the wild-type value, although it was about 10 and 20%, respectively, if the male cell lines had become established. In contrast, recA13 donors transmitted the chromosome with less than 10(-4) of the normal frequency. If chromosome transfer from F-lac(+) strains reflects the cutting and subsequent joining of homologous single strands of episomal and chromosomal deoxyribonucleic acid by recombination, these results imply that the completed unions are not made in recA cells, but can be effected with more than 50% of normal efficiency in newly formed partial diploids mutant at either recB or recC. Thus, the defective stage in recA mutants may precede strand joining, whereas the deficiency in recB or recC cells may involve a later step in recombinant formation.


Carbohydrate accumulation and metabolism in Escherichia coli: the close linkage and chromosomal location of ctr mutations. Six pleiotropic ctr mutations of Escherichia coli, affecting the ability to utilize 10 carbohydrates, were found to be closely linked to one another and to the mutation of strain MM6 causing lack of enzyme 1 of the phosphotransferase system. These mutations are located at 46 to 47 min on the E. coli map. Preliminary biochemical evidence indicates that the ctr mutants also lack enzyme 1, although they have a different phenotype from MM6.


Action spectrum for growth delay induced in Escherichia coli B-r by far-ultraviolet radiation. An action spectrum for growth delay induced in Escherichia coli B/r by far-ultraviolet radiation (230 to 295 nm) was obtained. It resembles the action spectrum for killing obtained in the same experiments, indicating that the chromophore for growth delay is probably the same as the chromophore for killing. Another action spectrum for killing, obtained under conditions more suitable for chromophore identification, suggests that nucleic acid, either deoxyribonucleic acid or ribonucleic acid, is the chromophore for growth delay induced by far ultraviolet. Isoprenoid quinones, which seem to be important chromophores for growth delay induced by near-ultraviolet radiation (above 300 nm), appear to play a negligible role in growth delay induced by wavelengths below 300 nm.


Polynucleotide sequence relationships among members of Enterobacteriaceae. Polynucleotide relationships were examined among many representatives of the Enterobacteriaceae by means of agar, membrane filter, and hydroxyapatite procedures. The amount of deoxyribonucleic acid (DNA) that reassociated was dependent, especially in interspecific reactions, on the annealing temperature. In only three cases: Escherichia coli-Shigella flexneri, Salmonella typhimurium-S. typhi, and Proteus mirabilis-P. vulgaris, was relative interspecific duplex formation 80% or higher. In most cases interspecies DNA duplex formation was 40% or less of that obtained from intraspecies DNA reassociation reactions. The stability of E. coli-S. flexneri DNA duplexes formed at either 60 or 75 C was virtually identical to that of homologous E. coli DNA duplexes, and the degree of interspecies duplex formation was minimally affected by the temperature increase (86% at 60 C; 77% at 75 C). The thermal stability of DNA duplexes formed at 60 C between DNA from E. coli and DNA from strains of Aerobacter aerogenes, S. typhimurium, S. typhi, and P. mirabilis was about 12 to 14 C below that of reassociated E. coli DNA. At 75 C, the formation of the interspecific DNA duplexes was markedly decreased, but the stability of the DNA able to reassociate at this temperature approximated that of reassociated E. coli DNA. The degree of reassociation and the thermal stability of E. coli-S. flexneri DNA duplexes suggests relatively little evolutionary divergence in these organisms. The other enterobacteria tested, however, have diverged to a point where less than one-half of their DNA can reanneal with E. coli DNA at 60 C and less than 10% reacts at 75 C. The degree of divergence between various enterobacteria does not appear to be uniform along the DNA molecule. Ribosomal ribonucleic acid (RNA)-specific sequences are conserved among most enterobacteria. An examination of messenger RNA relatively specific for the lactose operon suggests that specific chromosomal genes may diverge more or less than the genome as a whole.


Intraphagocytic degradation of group A streptococci: electron microsopic studies. The morphological changes that occur during intraphagocytic digestion of group A streptococci were studied by electron microscopy The first evidence of degradation of the ingested organism was the appearance of reticular changes in the bacterial endoplasm. This was followed by gradual swelling and dissolution of the bacterial wall, with final degradation of all the constitutuents to electron-dense debris. Accompanying changes in the phagocytic cell were observed; they consisted of vacuole formation, fusion of lysosomes with the wall of the yacuole, release of the lysosomal contents into the vacuole, and aggregation of the lysosomal contents around the ingested organism. Changes in the morphology of the organism similar to those observed during intraphagocytic digestion were also obtained by subjecting streptococcal cells to the action of the phage-associated lysin.


Surface features of Bacillus polymyxa spores as revealed by scanning electron microscopy. The surface features of Bacillus polymyxa spores were compared by use of thin sections, carbon replicas, and the scanning electron microscope. Some features of the characteristic ridges, previously reported in ultrathin sections and carbon replicas of spores of this species, were more clearly revealed with the scanning electron microscope. A three-dimensional image is provided because of the greater depth of focus possible with this instrument. End-on views of B. polymyxa spores readily illustrate the polygonal porelike structure present.


Surface structure of Bacillus stearothermophilus ribosomes. No significant differences were noted in either the size or in the arrangement of the surface filaments of the ribosomes of Bacillus stearothermophilus and Escherichia coli.


Effects of oleate starvation in a fatty acid auxotroph of Escherichia coli K-12. The effects of oleate starvation on an oleate auxotroph of Escherichia coli K-12 were investigated. Following removal of oleate from the mutant growing in a minimal glycerol-peptone medium, the cells stopped making deoxyribonucleic acid, ribonucleic acid, protein, and phospholipids; they began to die exponentially and finally lysed. During oleate starvation in minimal medium minus peptone, inhibition of macromolecular syntheses and death occurred; however, lysis did not follow. When growth ceased, no further dying was observed. It is shown that none of the early effects (inhibition of macromolecular syntheses and death) can be due to leakiness of the cells, induction of a prophage or a colicin, or lack of energy sources. The cause of inhibition of macromolecular syntheses remained unknown. Since the rate of death was the same as the generation time under different conditions, it appears that death is due to the defective synthesis of some cellular structure (quite possibly, cytoplasmic membrane) during phospholipid deficiency. Lysis was found to require protein synthesis; electron microscopy revealed a peculiar type of "lysis from within"; i.e., the shape of the cells did not change but fragmentation of the inner layer of the cell envelope occurred. The murein was found to be unaltered. Most likely, lysis was a consequence of the cell's attempt to synthesize cytoplasmic membrane with altered phospholipid composition or during phospholipid deficiency. Several membrane functions (respiration, adenosine triphosphate formation, permeability) existing before oleate removal were not lost during starvation. Therefore, general damage to the membrane did not occur, and it could be that most, if not all, described effects were due to defective de novo membrane synthesis.


Mapping of a structural gene for valyl-transfer ribonucleic acid synthetase in Escherichia coli by transduction. A structural gene, valS, for the valyl-transfer ribonucleic acid synthetase of Escherichia coli has been mapped on the clockwise side of pyrB and is closely linked to it.


Nonrandom mutagenesis of the Escherichia coli genome by nitrosoguanidine. The distribution on the genetic map of mutations induced with nitrosoguanidine in stationary-phase cultures of Escherichia coli is nonrandom.


Effect of vibriocins on members of the Enterobacteriaceae. Certain vibriocin producers exhibited antibacterial activity throughout the Enterobacteriaceae. To examine this phenomenon, an effective technique for demonstrating vibriocin production was developed.


Directed imagination method for projective assessment of teacher candidates.


Francis Gano Benedict--a biographical sketch (1870-1957).


The response of rat hypoglossal neurones to injection of botulinum toxin into the tongue.


Measurement of the volume shrinkage of lung tissue due to rapid freezing followed by freeze substitution.


Organization of whisker responses in the rat thalamus.


A new approach to freezing tissues rapidly.


The levels hypothesis: a re-examination and reorientation.


The quest for charisma.

The quest for charisma.


Group administration of the Hand Test: effects on card pull and responses.


Effects of subject, experimenter and task variables on compliance with the experimenter's expectation.


Self-perception and the Draw-a-Person Test.


An illustration of the Wechsler picture arrangement subtest as a thematic technique.


Houston administrator talks about heart transplants.


Task force urges Medicare outpatient drug coverage.


Inhibition of bone growth in vitro by endotoxin: histamine effect.


Total body water and weight changes in normal adults.


Tetanus prophylaxis in the U.S.

Tetanus prophylaxis in the U.S.


Massive hemolysis during sickle cell crisis.


The effect of glycerination on the fibrillar flight muscles of belostomatid water-bugs.


Folate activity and ascorbic acid in serum of anemic pregnant Nigerian women.


Metabolism of ascorbic-1-14C acid in experimental human scurvy.


Intrinsic factors in meat products counteracting botulinogenic conditions.


Incidence of resistance to tetracycline, chloramphenicol and ampicillin among Salmonella species isolated in The Netherlands in 1965 and 1966.


Cellular defense in an Arthropod in response to infection with a Salmonella typhimurium strain.


Temperature range for formic hydrogenylase induction and activity in psychrophilic and mesophilic bacteria.


The rapid loss of viability of Azotobacter in aqueous solutions.


Survival of Escherichia coli after storage in various frozen menstrua.


"Of trials".

"Of trials".


Australia and the future in Asia.

Australia and the future in Asia.


The centenary of nitrous oxide anaesthesia in England.


The diagnosis and treatment of mouth cancer.


Nifuratel in urinary infections.

Nifuratel in urinary infections.


Giant hydronephrosis in crossed unfused ectopic kidney presenting as an unusual abdominal swelling.