Tooth-supported complete dentures: description and clinical evaluation of a simplified technique.


The management of the prostatic nodule.

The management of the prostatic nodule.


Drug-induced lupus syndromes.

Drug-induced lupus syndromes.


The lumbar disc syndrome.

The lumbar disc syndrome.


Factors affecting maximal acid secretion.


The evidence that ganglioside, a metal, and chemical energy are involved in the serotonin household of synaptic vesicles from brain.


The choroid plexus as a glucose barrier.


Mechanisms of metabolite transport in various tissues.


The intracerebral movement of proteins injected into blood and cerebrospinal fluid of mice.


A comparison of the transport systems for amino acids in brain, intestine, kidney and tumour.


Changes in barrier effect in pathological states.


Transport inhibition phenomena in unilateral chemical injury of blood-brain barrier.


Changes in brain accumulation of amino acids and adenine associated with changes in the physiologic state.


Electron microscopic cytochemistry and microgasometric analysis of cholinesterase in the nervous system.


Studies on the effectiveness of antidepressant drugs.


On the problem of the harmful effect of DDT and its mechanism of action.


Central efferent processes and autonomic-behavioral integration.


The metabolism of diene-organochlorine (cyclodiene) insecticides.


HB419 (glibenclamide) in the treatment of diabetes mellitus.


Leprosy.

Leprosy.


Lethal effects of long wavelength ultraviolet and visible light on cells. ANL-7535.


Coefficient of relationship and histopathology in skin graft survival. ANL-7535.


The freeze-etch specimen preparation technique for electron microscopy. ANL-7535.


Sialography. New application of the subtraction technique.


Labelled compound related to synkavit and its uptake in certain human tumours studied by radio-isotope scanning.


Metabolic disorders of the ovary.

Metabolic disorders of the ovary.


Intraventricular trifascicular blocks. Review of the literature and classification.


The effects of small group interaction of sociometric choices of day campers.


Motor proficiency and other variables in educable mentally retarded children.


Mammalian microcirculation in the living animal. Microcirculation of renal homograft transplants in hamsters.


Attachment and penetration of Escherichia coli into intestinal epithelium of the ileum in newborn pigs.


Porphyria. The ole of iron and steroids.


Stellate plant hair contaminant in the laboratory.


The bacterial flora of peripheral vascular ulcers.


Adverse effects of topical steroids.

Adverse effects of topical steroids.


Response to the adenylate energy charge by Escherichia coli aspartate transcarbamylase and its catalytic subunit.


The in vitro synthesis of 2'-omethylguanosine and 2-methylthio 6N (gamma,gamma, dimethylallyl) adenosine in transfer RNA of Escherichia coli.


Interaction of human growth hormone and human erythrocytes membranes as demonstrated by circular dichroism.


On the specificity of the two ribosomal binding sites: studies with tetracycline.


The phosphoglyceride composition of Gram-negative bacteria and the changes in composition during growth.


Modesl of growth in mammalian cells.

Modesl of growth in mammalian cells. The increase of cell volume as a function of time was studied throughout the generation cycle in synchronous cultures of Chinese hamster cells using a Coulter aperture and a multichannel analyzer calibrated against known cell volumes. The experimental results were compared to a mathematical model of cell volume increase which considered the effect of the distribution of individual cell generation times on the progress of the population. Several modes of volume increase, including linear and exponential, were considered. The mean volume vs. time curve was rounded at the ends of the cycle even when linear growth was assumed. The experimental results show that cell volume increased in a smooth fashion as a function of time, with no discontinuities in rate detectable at periods when cells may have been undergoing metabolic shifts as, for example, through the phases associated with DNA synthesis, G(1), S, G(2). A statistical test on the comparison of the modal cell volume vs. time data to the predictions of linear and exponential growth models accepted both hypotheses within the resolution of these experiments. However, exponential growth was favored over linear growth in one cell line. Volume dispersion was almost constant with time in both sublines which is also consistent with exponential growth. Limitations of the electronic technique of volume measurement and indications for future experiments are discussed.


Receptive fields in visual systems.

Receptive fields in visual systems.


Codeine and its alternates for pain and cough relief. 5. Discussion and summary. This chapter concludes the survey of experimental and clinical data on the analgesic and antitussive properties of codeine and its potential therapeutic alternates. From an evaluation of their effectiveness on the one hand and the side-effects, including tolerance, dependence and abuse liability on the other, it would appear that the therapeutic goals of codeine could be achieved by other substances, except perhaps where analgesia, cough relief, and sedation are required simultaneously. The use of these other substances would, however, result in no particular gain and probably no particular loss.


Dietary protein and pesticide toxicity in male weanling rats. The studies reviewed in this paper were undertaken at the request of the World Health Organization to obtain information on pesticides that would be least toxic for use in countries where the diet is deficient in protein. To investigate the problem, the acute oral LD(50) and the associated clinicopathological syndrome of toxicity were measured in weanling male albino rats fed for 28 days from the time of weaning on diets containing different amounts and different types of proteins. Initial results suggested that the type of dietary protein was of minor importance provided it was present in adequate amounts. Using casein as a source of dietary protein, it was found that the toxicity of 5 pesticides was not appreciably increased when the amount of dietary casein was reduced to one-third of normal requirements. When dietary casein was reduced to 13% of the requirements for normal growth, the toxicity of all 13 pesticides under study was increased. The greatest increases were associated with carbaryl, parathion and, particularly, captan. When dietary casein was increased to 3 times the normal amount, the toxicity of some pesticides such as DDT was augmented while that of others such as carbaryl was unaltered.


New prospects for the study of leprosy in the laboratory. Although Mycobacterium leprae was identified earlier than Myco. tuberculosis, it has still not been cultured in vitro and only in 1960 was an infection obtained in laboratory animals. However, important advances have been made in the field of experimental leprosy in the last decade due to the development of new techniques and models for studying Myco. leprae in vivo, thus overcoming the limitations imposed by a non-cultivable mycobacterium. Quantitative techniques using Myco. lepraemurium provided the first model for developing an indirect method for distinguishing dead (non-infectious) from living (infectious) bacilli, based on morphological differences in organisms stained by the Ziehl-Neelsen method. However, the most important advances resulted from the limited and localized growth of Myco. leprae when inoculated into the foot pads of mice and, later, the more substantial and generalized multiplication of Myco. leprae in immunologically deficient mice (thymectomized and irradiated with a dose of 900 r). Moreover, in the immunologically deficient animals, the infection eventually resulted in a disease replicating that of lepromatous type leprosy in man, including the involvement of peripheral nerves.The results from these studies and the future prospects for the study of leprosy in the laboratory are reviewed in this article.


Dr. René Charbonneau: 1914-68. C.O.S. president June '63 to Oct. '64.


Indomethacin and acetylsalicylic acid in the treatment of osteoarthritis of the hips and knees.


Conduction of the nerve impulse.

Conduction of the nerve impulse.


The American Cancer Society and Cancer Research Origins and Organization: 1913-1943.


Electrocardiographic studies of cardiac orthotopic allotransplantation. 2. The post-operative puppy.


Coding response of an altered phenylalanine transfer RNA from an Ehrlich ascites tumor.


Registry of U. S. nephrology training centers.


A linear reflection densitometer for indocyanine green.


Intrarenal volumes of distribution and relative diffusion coefficients of monohydric alcohols.


Correlation of whole-body impedance with total body water volume.


Cardiovascular responses of the unanesthetized and unrestrained hypothermic rat.


An automatic control device to regulate at subzero temperatures.


Computer evaluation of acid-base disorders. With the advent of electronic computers that operate in the time-sharing mode, it has become possible to develop an automated system that can assist a physician in solving clinical problems. In the present study a teletype terminal has been linked to a time-sharing computer which has been programmed to evaluate clinical and laboratory information concerning patients with acid-base disorders. The program checks the data for evidence of internal consistency and requests additional information as needed to solve the acid-base aspects of the clinical problem. If sufficient information is provided, the program generates an evaluation note designed to review with the physician the pathophysiology of the disorder and to assist him in its management. If the input data are incomplete, the program draws the most useful conclusions possible based on the data provided, specifies the limitations which pertain to these conclusions, suggests further studies designed to circumvent these limitations, and while awaiting the results, suggests appropriate interim therapeutic measures. The time required to enter a patient's data and to print the evaluation note is approximately 4 min; the cost is comparable to that of many laboratory tests.


Institutionalization of the aged: effects on behavior.


Continuous infusion indicator dilution measurement of limb blood flow and vascular response to magnesium sulfate in normotensive and hypotensive men. A constant infusion, indicator dilution technique for blood flow measurements in the forearm and hand of man was tested and validated in vitro and in vivo. This technique employs jet injection to improve mixing of indicator with arterial blood. The mixing characteristics of the jet injection system were studied in vitro in tubing simulating the brachial artery of man. In addition, actual blood flows in the isolated pump-perfused forelimbs of five dogs were compared with constant infusion, indicator dilution calculated flows. Measurements were also made of mixing and of blood flow in the forearm and hand of man. The technique was used to compare forearm and hand vascular responses with constant intrabrachial arterial infusions of magnesium sulfate in 13 normotensive and 13 essential hypertensive men. In vitro and in vivo the jet injection system significantly improved mixing of indicator with blood, as compared with mixing produced by standard infusion techniques, without causing hemolysis. In 30 measurements in isolated, perfused dog forelimbs the correlation coefficient between actual and calculated blood flow was 0.992. Resting limb vascular resistance in the hypertensive group was significantly higher than in the normotensive group. Limb vascular resistance in all 26 men decreased in response to intrabrachial-arterial infusion of 0.25% magnesium sulfate (8 ml/min). Rate of infusion of Mg(++) was 0.162 mEq/min. There was a significant positive linear correlation between level of initial limb vascular resistance and magnitude of response to magnesium sulfate. Vascular response data adjusted for this source of variation were similar in hypertensives and normotensives.The data suggest that this constant infusion, indicator dilution technique allows accurate calculation of total limb blood flow in man, provided that anomalous bifurcation of the brachial artery is not present. The data also suggest that the jet injection system improves mixing of substances with arterial blood. Thus, use of this system should especially aid reliability of studies of limb vascular responses to vasoactive agents infused into the brachial artery.


Current publications in gerontology and geriatrics.


Cellular response of mice to infection with Pasteurella tularensis (live vaccine strain) following continuous exposure to low dose gamma radiation.


Studies of intestinal microflora. 8. Effect of lincomycin on the microbial population of the human intestine.


Fluroescent antibody test for the serodiagnosis of Theileria annulata.


International Leprosy Association.

International Leprosy Association.


Natural and acquired antibodies to Plasmodium lophurae in intact and bursaless chickens. II. Immunofluorescent studies.


A study of the adrenal medulla and its subcellular organelles by the freeze-etching method.


Chemotherapeutic trials in leprosy. 6. Pilot study of the riminophenazine derivative B.663 in low dosage (100 mgm. twice weekly) in the treatment of lepromatous leprosy.


Ultramicroscopic filaments in the ascidian Botryllus schlosseri (Pallas) and their possible role in ampullar contractions.


Use of pediatric cadaver kidneys for homotransplantation in children.


Congestive heart failure--acute pulmonary edema.


Determination of blood volume using indocyanine green (cardio-green) dye.


Microincineration as a new method for electronmicroscopical investigations of bull sperm.


Irradiation as an immunosuppressive agent in organ transplantation.


Angiographic investigation of formation of thrombi on vascular catheters.


John D. Camp, M.D. 1898-1969.

John D. Camp, M.D. 1898-1969.


Erwin Beck, M.D., F.A.C.R. 1909-1968.

Erwin Beck, M.D., F.A.C.R. 1909-1968.


Depression and allergic dermatologic disease.


Vincent Marvin Ravel, M.D. 1914-1969.

Vincent Marvin Ravel, M.D. 1914-1969.


Clinical and EEG studies of doxepin. An interim report.


Width of azygos vein related to central venous pressure.


B-scan ultrasonography of abdominal mass lesions.


The roentgen evaluation of the urinary tract following treatment for vesicoureteral reflux.


Antideessant activity of doxepin and amitriptyline. A double-blind evaluation.


Severe emotional disturbance associated with physical illness. I. Double-blind study.


Automation in diagnostic radiology--a critique.


John Westgate Hope, M.D. 1914-1969.

John Westgate Hope, M.D. 1914-1969.


Joseph W. Post, M.D. 1887-1969.

Joseph W. Post, M.D. 1887-1969.


Milton LeVine, M.D. 1918-1969.

Milton LeVine, M.D. 1918-1969.


Management of psychoneurotic anxiety and depression in outpatients.


Legal liability in new drug investigation.


Heterophil antibodies in the sera of recipients in clinical renal transplantation.


Failure of phytohemagglutinin to enhance azathioprine suppression of renal homograft rejection.


The comparative in vitro and in vivo activity of antilymphocyte serum raised by immunization with thymic, splenic, and lymph node lymphocytes.


The peptic ulcer problem.

The peptic ulcer problem.


Short term clinical evaluation of a clay containing dentifrice. 565.


The biology of the acanthocephala.

The biology of the acanthocephala.


The post-embryonic developmental stages of cestodes.


Evolutionary trends in mammalian trypanosomes.


Problems in the cultivation of some parasitic protozoa.


U.S. Army Surgical Research Program. Recent progress and current interests.


Synthesis and properties of ribosylpyrimidin-2-one.


Membrane expansion of erythrocyte ghosts by tranquilizers and anesthetics.


The muscle proteins of the vascular wall.


Peptidyl-tRNA. VII. Substrate specificity of peptidyl-tRNA hydrolase.


Studies on ribosomal RNA from Tetrahymena by zone velocity sedimentation in sucrose gradients and base ratio analysis.


Use of reversed-phase column chromatography for rapid isolation and identification of formylmethionyl transfer RNA.


Effect of 9 alpha-fluoroprednisolone and L-asparaginase on uridine incorporation into ribosomal RNA of P1798 lymphosarcoma.


Changes in physical properties of stored erythrocytes relationship to survival in vivo.


Isolation and sucrose density gradient centrifugation of ribosomal and mitochondrial RNA from yeast.


Requirements for ribosomal peptide bond-forming activity in the fragment reaction.


Size, structure and genetic complexity of yeast mitochondrial DNA.


Influence of relative humidity on the photochemistry of DNA films.


Messenger RNA concentration and protein synthesis during T4 infection of Escherichia coli B.


Transient repression of beta-galactosidase synthesis: effect of growth history.


Effect of denatured DNA's on Salmonella typhimurium ribonuclease I.


Isolation of circular DNA by zonal centrifugation. Separation of normallength, double length and catenated M13 replicative form DNA and of host specific "episomal" DNA.


arotid-cavernous arteriovenous fistula.

arotid-cavernous arteriovenous fistula.


Organ conservation, surgical technique and immunosuppressive therapy in kidney transplantation.


The effect of tolbutamide on the diabetic fatty liver.


The use of fluorescent antibodiein e identification of streptococci. 3. Streptococcus B group typing by means of the precipitation and immunofluorescence methods.


Spermatozoa-coating antigen (SCA): its persistence in the genital tract of female rabbits.


Cytotoxic effect of antispermatic antibodies on guinea pig germinal cells in vitro.


Malnutrition and mental behaviour.

Malnutrition and mental behaviour.


Nutritional status--U.S.A.

Nutritional status--U.S.A.


Antenatal diagnosis of cytogenetic and biochemical disorders.


Dietary caffeine and serum lipid levels in the rat.


Pyelonephritis.

Pyelonephritis.


Potential interferences in certain pesticide residue analyses from organochlorine compounds occurring naturally in plants.


In memoriam Marshall Howritz M.D.

In memoriam Marshall Howritz M.D.


Recent results of human cytogenetics. Clinical significance of chromosomal studies.


The assessment of endothelial viability.


A case of reticulum cell sarcoma in the brain of a dog.


Studies in oral leukoplakias. XVII. Lactate dehydrogenase isoenzyme patterns in oral leukoplakia.


Parathyroid abnormalities. A histological study witspecial reference to sudden, unexpected death in infancy.


Visna and maedi virus antigen in infected cell cultures studied by the fluorescent antibody technique.


Renal function in gout. 3. Estimation of tubular secretion and reabsorption of uric acid by use of pyrazinamide (pyrazinoic acid).


Osmium binding to the surface coat of intestinal microvilli in the cat under various conditions.


American Association of Anatomists.

American Association of Anatomists.


Recurrent and alternating Bell's palsy.

Recurrent and alternating Bell's palsy.


Early days of antimicrobialherapy.

Early days of antimicrobialherapy.


Interactions of small molecules with nucleic acids. I. Mode of action of anthramycin.


Unusual pattern of tetracycline resistance in Shigellae mediated by resistance-transfer factors.


Effect of topical antibacterial agents on the acquisition of bacteria by newborn infants.


Laboratory and clinical evaluation of carbenicillin (carboxybenzyl penicillin).


Contribution of R factors to the antibiotic resistance of hospital isolates of Serratia.


Inhibition of messenger ribonucleic acid attachment to ribosomes. II. Proposed mechanisms for the design of novel antiviral agents.


In vitro activity of carbenicillin against gram-negative bacilli.


Enhanced activity of novobiocin against gram-negative bacilli in acid media.


Inhibition of resistance-factor transfer by clindamycin and its analogues.


Strain differences for titration of bactericidal Escherichia coli antibodies in serum of infected dogs.


Mechanism of suppression of resistance development by an antibiotic combination.


Antibiotic therapy of Escherichia coli pyelonephritis produced in mice undergoing chronic diuresis.


2-Amino-5-(1-methyl-5-nitro-2-imidazolyl)-1,3,4-thiadiazole, a new antimicrobial agents. IV. Activity in mice and chicks.


Hemolysin production in Escherichia coli associated with nalidixic acid resistance.


Etiology of psychiatric disorders in work organizations.


American Academy of Occupational Medicine 1969 Award of Merit to John L. Norris, MD.


Pulmonary effects of air pollution.

Pulmonary effects of air pollution.


Parathyroid gland metabolism.

Parathyroid gland metabolism.


Successful renal allograft in a patient with pulmonary cryptococcus.


Sir Francis Gordon Bell.

Sir Francis Gordon Bell.


Immunological aspects of renal transplantation.


The development of renal transplantation--a review.


The influence of technical factors upon renal homograft function.


Forty years on: something of South Sea surgery and surgeons.


Preparation of patients for cadaveric renal transplantation.


A comparison of uretero-ureteric and ureterovesical anastomosis in renal ansplantation.


Hepatic transplantation in man.

Hepatic transplantation in man.


Early function in renal allografts from cadaver donors.


Avascular necrosis in cadaveric renal allografts.


Suppression of canine renal allograft rejection with antilymphocyte serum.


Human cardiac allotransplantation: a case report.


Pancreatic autotransplantation in sheep.


Peptic ulcer: medical treatment.

Peptic ulcer: medical treatment.


Malabsorption and gastro-intestinal surgery.


Hazard of self-inflating resuscitation bags.


Relevance of the electronic computer to hospital medical records. During the past 30 years an "information explosion" has completely changed patterns of illness. Unit files of individual patients have become so large that they are increasingly difficult both to store physically and to assimilate mentally. We have reached a communications barriers which poses a major threat to the efficient practice of clinical medicine.At the same time a new kind of machine, the electronic digital computer, which was invented only 26 years ago, has already come to dominate large areas of military, scientific, commercial, and industrial activity. Its supremacy rests on its ability to perform any data procedure automatically and incredibly quickly.Computers are being employed in clinical medicine in hospitals for various purposes. They can act as arithmetic calculators, they can process and analyse output from recording devices, and they can make possible the automation of various machine systems.However, in the field of case records their role is much less well defined, for here the organization of data as a preliminary to computer input is the real stumbling-block. Data banks of retrospective selected clinical information have been in operation in some centres for a number of years. Attempts are now being made to design computerized "total information systems" to replace conventional paper records, and the possibility of automated diagnosis is being seriously discussed.In my view, however, the medical profession is in danger of being dazzled by optimistic claims about the usefulness of computers in case record processing. The solution to the present problems of record storage and handling is very simple, and does not involve computerization.


White blood count in patients on regular haemodialysis. Total white cell, neutrophil, and lymphocyte counts were compared in patients with chronic renal failure treated successively by conservative means (low-protein diet), regular haemodialysis, and renal allotransplantation. The lowest total white cell and neutrophil counts and the highest lymphocyte counts were found in patients on regular haemodialysis. A rapid fall in neutrophil count during the first half-hour of dialysis and a more gradual fall between the first and sixth hours were observed. Adherence of neutrophils and mononuclear cells to the cuprophane (PT 150) dialysis membrane has been shown.


America's unhealthy twins.

America's unhealthy twins.


Plasma factor IX levels in patients given hexoestrol or stilboestrol to suppress lactation. A single injection of hexoestrol, 45 mg., is effective in suppressing lactation. Given in this way hexoestrol causes a small rise in plasma factor IX levels, of shorter duration than that produced by a customary and equally effective oral course of stilboestrol. With hexoestrol the plasma factor IX levels reverted to normal by the sixth day of the puerperium.


Disodium cromoglycate in exercise-induced asthma.


Sir Frederic Bartlett, C.B.E., M.A., D.SC., F.R.S.


Laboratory application of the dextran-coated-charcoal radioimmunoassay of insulin.


Preliminary x-ray diffraction study of the wild-type and a mutationally-altered tryptophan synthetase alpha-subunit.


National drug code directory.

National drug code directory.


Radial-innervated cross-finger flap from index to provide sensory pulp to injured thumb.


Sensory cross-finger pedicle graft.

Sensory cross-finger pedicle graft.


Impacted fractures in the proximal portion of the proximal phalanx of the finger.


The role of Alessandro Codivilla in the development of skeletal traction.


Philip Duncan Wilson, M.D. 1886-1969.

Philip Duncan Wilson, M.D. 1886-1969.


Current concepts of local excision of epidermoid carcinoma of the anus.


The use of five-millimeter Mersilene woven strip in the Thiersch operation.


Skin grafts in surgery for anal fistula.


Shakespeare's cutaneous curses and contempt.


The use of isoproterenol in the treatment of the acute asthmatic attack with report of case.


Treated late syphilis: immunoglobulin class of antibodies reactive with Treponema pallidum.


Early events of virus-cell interaction in an adenovirus system. The interaction of (32)P-labeled adenovirus type 2 and HeLa or KB cells has been examined during early infection. The kinetics of virus uncoating to deoxyribonuclease-sensitive products, the partial characterization of three such products by gradient centrifugation, and the distribution of these products in the extranuclear and nuclear portions of infected cells are reported. The results are compatible with the following model. Extracellular virus attaches to a receptor on the plasma membrane. The membrane-bound virus has a half-life of less than 15 min and is transformed to a partly uncoated product which is free inside the cell and about half of which rapidly enters the cell nucleus. This is rapidly transformed, in both cytoplasm and nucleus, to a membrane-bound virion "core." The proteins of the bound "core" are then removed from the intact virus deoxyribonucleic acid (DNA). In the nucleus, viral DNA is the main product and there the overall sequence is completed in about 2 hr.


Synthesis of replicative form deoxyribonucleic acid and messenger ribonucleic acid by gene IV mutants of bacteriophage S13. Gene IV mutants of bacteriophage S13 are known to be blocked in infectious replicative form (RF) DNA synthesis, producing only a small fraction of the RF formed by wild-type phage. This investigation shows that gene IV mutants form only parental RF and are blocked in the synthesis of any progeny RF, either infectious or noninfectious. This was determined by density labeling of RF in cells treated with mitomycin C to suppress host deoxyribonucleic acid (DNA) synthesis. RF synthesis was also studied in untreated cells, using methylated albumin columns to separate RF from host DNA. In this case it was also found that synthesis of progeny RF by gene IV mutants is negligible. It has been found by DNA-ribonucleic acid (RNA) hybridization experiments that gene IV mutants form at least as much or more messenger RNA than wild-type phage. Therefore, parental RF alone can form messenger RNA in appreciable amounts.


Anomalous induction of mycobacteriophages mediated by mitomycin C. Mitomycin C has been found to stimulate the production of long-tailed defective bacteriophages and poly tails in thick cell wall mycobacterial mutants.


A simple mathematical derivation of the Stewart-Hamilton formula for the determination of cardiac output.


Primary structure of transfer RNA.

Primary structure of transfer RNA.


The liver in protein-calorie malnutrition.


Nutrition in renal failure.

Nutrition in renal failure.


Ammonium salts and nitrogen metabolism in man.


Iron and erythropoiesis.

Iron and erythropoiesis.


Iron deficiency and gastrointestinal lesions.


The influences of amino acid deficiencies on hepatic protein synthesis.


Factors causing changes in plasma amino acid patterns.


Heat regulation: homeostasis of central temperature in man. Focus is on the concept of the quantitative thermostat or setpoint as it evolved experimentally, not theoretically, from measurements of central temperature at the tympanic membrane and from measurements of physiological responses by human gradient layer calorimetry and continuous analysis of oxygen consumption.  Heat-flow responses as they relate to stimuli by which they are evoked -- peripheral or central temperatures at thermoreceptive sites -- and discussed, and individual systems of reflex action are described.  These systems are then correlated with what is known of their tangible components -- sensory receptors, effector organs, nervous centers, and afferent or efferent nervous pathways -- classically derived from anatomical studies, lesion experiments, recording of action currents, or electrical or thermal stimulation of pertinent structures.  Such as correlation is a crucial test, and when the results are consistent, they provide independent, mutual confirmation via the 2 approaches.  Doubts are then removed that lesions or interferences, which are unavoidable with most neurophysiological methods, may have produced artifacts.  Conversely, calorimetry allows one to observe and analyze thermoregulation in process in a human subject without disturbing normal structures or functions other than by the stresses to which the body is made to respond.  Prior to a description of experimental findings, the essential methods are reviewed.  With the methods of gradient layer calorimetry, tympanic (eardrum) thermonetry, and indirect calorimetry, thermoregulatory responses -- by chemical overproduction of metabolic heat -- were observed in transient states as a result of a stimulus of cold impinging on peripheral neurons or warm-inhibition removed from central structures.  With tympanic thermometry permitting for the 1st time om humans the continuous recording of central thermal stimulation, and with gradient calorimetry permitting the continuous recording (with insignificant inertial distortion) of the sweating responses in precisely controlled environments of a large variety and under conditions where complete evaporation of sweat is ensured by strong but smooth air convection, a new study of physical thermoregulation was initiated in 1958.  The experimental results did not support the expection that sweating would follow the average temperature of the skin in the classical tradition, with skin warm-receptors as the hypothetical origin of exciting warm-impulses and cenral temperature as a participant prescribing the action of the "thermoregulator" in some as yet undetermined, additive, manner.  Vasomotor mechanisms to human thermoregulation can be assessed in quantitative manner only by whole-body calorimetry.  Mechanisms of thermal homeostasis also considered include hormonal regulation, transmitter substances, conscious sensation of warmth and coordinations of behavioral and autonomic mechanisms, setpoint and its variations, and fever.


Crystal chemistry of bone mineral.

Crystal chemistry of bone mineral.


Rheoencephalography: present status.

Rheoencephalography: present status.


Rheology of blood.

Rheology of blood.


Pathogenesis of hypertensive intracerebral hemorrhage: a hypothesis with supporting data.


Extracellular pH in the brain and cerebral blood flow.


Delayed radiation effects in atomic-bomb survivors. Major observations by the Atomic Bomb Casualty Commission are evaluated.


Cell sorting: automated separation of mammalian cells as a function of intracellular fluorescence. A system for high-speed sorting of fluorescent cells was able to sort mouse spleen cells from Chinese hamster ovarian cells after development of fluorochromasia. Highly fluorescent fractions separated after similar treatment from mouse spleen cells immunized to sheep erythrocytes were enriched in antibody-producing cells by factors of 4 to 10.


Extensive ulcerations in the postphlebitic leg.


Surgical treatment for hidradenitis suppurativa.


Symptoms, septicemia and death in salmonellosis.


Bacteriuria in noninfectious renal disease.


The life cycle of virulent toxoplasma in cell cultures.


A new assay for phosphodeoxyribomutase: surface localisation of the enzyme.


Comparative studies of bacterial hydrogenase.


Mechanism of the salicylate hydroxylase reaction. II. The enzyme-substrate complex.


The influence of monovalent cations and hydrostatic pressure on beta-galactosidase activity.


John Shaw Billings as a Bibliographer.

John Shaw Billings as a Bibliographer. The influences that a man's childhood have on his life are, it is well known, great. Life is essentially a part of the things that happen to the individual and it is the manner in which one relates oneself to these things that determines what one is. With these facts in mind this study of John Shaw Billings as a bibliographer has been approached. His early life has been reviewed as an influence on his later achievements. Stress has been placed on those events which led to his bibliographic activities.Dr. Billings was prolific in many fields. Others have given detailed analyses of his writings (1, 2). The present study will consider only his bibliographic works. The description of these follows the brief outline of his childhood and youth.


Need for the Abridged Index Medicus.

Need for the Abridged Index Medicus.


An annotated bibliography of education for medical librarianship, 1940-1968. An attempt has been made in this bibliography to represent the various viewpoints concerning education for medical librarianship equally. The topics covered include: general background reading and readings for those interested in establishing courses in medical librarianship. The former includes annotations on the history and international aspects of the subject. The latter consists of annotations of articles on early courses and present courses in medical librarianship. A final area discussed is the Medical Library Association's Code for the Training and Certification of Medical Librarians.


Elliott How Morse.

Elliott How Morse.


Metabolism of 14C-histamine in domestic animals. I. Goat.


A comparison of two methods of assessing capillary permeability in isolated perfused muscle based on single injecion technique.


Capillary diffusion capacity for small hydrophilic molecules in the exercising forearm of man.


Blockade by triptyline compounds of tyramine uptake and of tyramine-induced phosphorylase activation in cardiac tissue.


Progress in disseminated intravascular coagulation.


Heart rate after cardiac transplantation.


Hemodynamic performance of a transplanted human heart.


The excretion of tritiated digoxin in normal human volunteers before and after unilateral nephrectomy.


USAF designs denture tags with social security numbers.


Organizer and first president of the New Jersey State Dental Society.


Editorial: Restraint by physicians as essential to future economic success of Medicare and Medicaid.


Cusps and fossae of dental practice.

Cusps and fossae of dental practice.


Microbial production of L-lysine.

Microbial production of L-lysine.


Antibiotics literature index--a select bibliography.


The history of psychiatry in Mexico.

The history of psychiatry in Mexico.


Pathology of amoebiasis--historical aspects.


Walter M. Dale, M.D., D.Sc. (1894-1969).


Factors affecting production of staphylococcal lipase.


Influence of the Maillard reaction products on some bacteria of the intestinal flora.


Medium volume and the survival of Serratia marcescens.


Action of biguanides, phenols and detergents on Escherichia coli and its sphereoplasts.


Calcium sensitive and other mutants of bacteriophage P2.


The surgical management of chronic duodenal ulcer.


A controlled trial of surgical induction of labour and amnioscopy in the management of prolonged pregnancy.


Noxytiolin (Gynaflex) in the preoperative preparation of the vagina.


Multiple myeloma: review and report of case.


Expanding with the "Scientific American".


The history and superstitions of birth defects. I.


Burns in my lifetime.

Burns in my lifetime.


Second thoughts on the burn wound.

Second thoughts on the burn wound.


Thoracic trauma in infancy and childhood.


Discussion of methods of management of lower extremity soft tissue trauma in arteriosclerotic patients.


Louis Pasteur (1822-1895).

Louis Pasteur (1822-1895).


Malignant lymphomas: further studies on their preferential sites of involvement and possible mode of spread.


A trial of disodium cromoglycate in older asthmatics.


Disodium cromoglycate in the treatment of seasonal and perennial asthma.


1969 Membership Directory. Greater Kansas City Dental Society.


The cholera epidemic in Detroit 1832 and 1834.


Lipid mobilization in relation to body composition in man.


The electronic structures and pharmacological activities of sulfanilamide derivatives and carbonic anhydrase inhibitors with the same sulfonamide moiety.


Studies on a deoxyribonucleic acid-quinine complex.


Theobald Smith, 1859-1934. Life and work.


Have times changed?

Have times changed?


Supporting those who do (Janet Graig).

Supporting those who do (Janet Graig).


Imagineering--a new concept.

Imagineering--a new concept.


Methodology of family and marital studies.


Some problems of family measurement.

Some problems of family measurement.


Scientific investigation of relationships and communication within the family.


Mechanisms of graft rejection.

Mechanisms of graft rejection.


Decreased ventilatory response to hypoxia during acute polycythemia in the calf.


The dry eye.

The dry eye.


A century of research on the metabolic role of zinc.


Metabolic role of zinc.

Metabolic role of zinc.


Skeletal and skin lesions of zinc-deficiency chickens and swine. Possible relationship to "connective tissue diseases" of man.


Glucose tolerance, free fatty acid, and serum insulin responses in patients with cirrhosis.


Surgical correction of vaginal agenesis.


Hormones and the liver. The effect of estrogens, progestins, and pregnancy on hepatic function.


George T. Pack, M.D., 1898-1969, a tribute.


Colon bacillus pneumonia: a complication of lesser sac abscess.


Ongoing assessment of therapy in septic arthritis.


The photochemistry of 4-thiouridine in Escherichia coli t-RNA Vał1.


Rifampicin inhibition of vaccinia replication.


Serum albumin.

Serum albumin.


Altered chromatographic properties of tRNA from chloramphenicol-treated Escherichia coli.


The hospital on the wall. Spafford Memorial Children's hospital, Jerusalem.


Dr. Alastair C. Frazer.

Dr. Alastair C. Frazer.


Metabolism of a tartrazine analogue by intestinal bacteria.


Tannic acid again.

Tannic acid again.


The problem of transferred resistance.

The problem of transferred resistance.


Hot air on PTFE.

Hot air on PTFE.


Carry on toxicologist.

Carry on toxicologist.


Elements traced to the blood.

Elements traced to the blood.


Introduction to endosseous implants in dentistry.


James C. Wallace, NDA past president, dies.


Gies Editorial Award won by Frank Compton.


Foley's footnotes.

Foley's footnotes.


M. W. Prince, first dental dean at Loma Linda, dies.


Designate birthplace of naval dentistry.


Impressions for complete dentures.

Impressions for complete dentures.


Cutting efficiency in complete coverage preparation.


The in vivo pH of the extravascular space of the lung. The partition of 5,5-dimethyloxazolidine-2,4-dione (DMO) and of 11 amines between the vascular and extravascular spaces of the lung has been determined by the multiple indicator dilution technique. Four amines (nicotine, pentylamine, quinine, and benzylamine) were found to have pH-sensitive tissue to blood concentration ratios. Of these, tritiated nicotine appears to be the nost satisfactory indicator of tissue pH and values for the pH of the pulmonary extravascular space (pH(e)) have been calculated from the nicotine data. At an arterial pH (pH(art)) between 7.38 and 7.43 pH(e) averaged 6.69 +/-0.07. Changes in pH(e) usually paralleled but were consistently less than concomitant changes in pH(art). Alterations in P(CO2) at constant pH(art) regularly produced relatively small, parallel changes in extravascular hydrogen ion concentrations. Local alterations in tissue pH due to P(CO2) changes are apparently buffered quite rapidly and the pH(e) of the lung seems more closely linked to pH(art) than the cellular pH of other tissues.DMO, guanidine, methylamine, morphine, and atropine were confined to the vascular volume during the first circulation and could not be used to measure tissue pH. Histamine appeared to be bound to a pH-insensitive site. The extravascular distributions of antipyrine and aniline were unresponsive to alterations in arterial pH, presumably because they are essentially uncharged at pH levels found in the lung.


Pneumothorax during infant resuscitation.


Friedrich Loeffler (1852-1915). Klebs-Loeffler bacillus.


The effects of hormones on the absorptive, metabolic and digestive functions of the small intestine.


Current bibliographic index of mammalian reproduction. X.


Early health departments.

Early health departments.


Royal operations.

Royal operations.


The carcinoid tumor, syndrome, and spectrum, a review of the literature.


An extrapleural approach to the heart with particular reference to pulmonary artery banding and innominate artery suspension.


Massachusetts medical society. Past, present and future.


Case records of the Massachusetts General Hospital Weekly clincopathological exercises. Case 46-1969.


St. Elizabeth's hospital centennial celebration.


Malabsorption syndrome.

Malabsorption syndrome.


El Camino real AIN honors nurse consultant.


How Winnipeg waged war on typhoid fever.


Sensory aspects of psychopathology.

Sensory aspects of psychopathology.


Antibacterial activity of sulfadimethoxine potentiated mixture (Ro 5-0013) in chickens.


Evoked potentials and psychopathology.

Evoked potentials and psychopathology.


Cytogenetic aspects of psychopathology.

Cytogenetic aspects of psychopathology.


Psychopharmacology and psychopathology.

Psychopharmacology and psychopathology.


A consideration of biological factors in schizophrenia.


Electrolytes and mental illness.

Electrolytes and mental illness.


Behavioral and affective responses to brain stimulation in man.


Psychomotility: a dimension of behavior disorder.


Joseph Zubin. Paul H. Hoch award lecturer, 1968.


Immunological reactions after irradiation of the uterus.


Use of antisludging agents in experimental cold injuries.


The bacteriologic study of surgical gloves from 250 operations.


Factors affecting the infection of anophelines with human malaria in Thailand.


Distribution of genetic red cell defects in south-east Asia.


Three miniature lung ventilators.

Three miniature lung ventilators.


Replacement of infected arterial prosthesis with inverted jejunal autografts.


The radical mastectomy incision.

The radical mastectomy incision.


Avulsions of the tactile finger pad: an evaluation of treatment.


Estimation of the number of critical sites in limited genome expression during viral infection of bacteria.


Psychopathology of Turner's syndrome.

Psychopathology of Turner's syndrome.


A steam-sterilizable electrode of the galvanic type for the measurement of dissolved oxygen.


The relationship between critical oxygen level and antibiotic synthesis of capreomycin and cephalosporin C.


Effects of oxygen and glucose levels on lipid composition of yeast Candida utilis grown in continuous culture.


The effect of Eh on regulatory processes in facultative anaerobes.


An improved French pressure cell.

An improved French pressure cell.


The paronym defect and verbal abstraction.


Characterization of DNA-like RNA in the Ilyanassa embryo.


The Presidential address.

The Presidential address.


A preliminary twin study in Taiwan. I. Epidemiological aspect.


Spina bifida occulta.

Spina bifida occulta.


A heat-labile enterotoxin from strains of Eschericha coli enteropathogenic for pigs.


The serum bactericidal reaction. IV. Phenotypic conversion of Escherichia coli from serum-resistance to serum-sensitivity by diphenylamine.


The effect of thorotrast on the resistance of mice to experimental infection with Escherichia coli and Salmonella typhimurium.


Coxsackievirus myocardiopathy.

Coxsackievirus myocardiopathy.


Effects of arterial hypertension and chronic dilatation of receptor area on aortic baroreceptor activity.


Transfer characteristics of the antagonistic regions of the retinal receptive fields.


The origin of human placental oxytocinase.


Projection of the caudate nucleus on the anterior lobe of the cerebellum of the cat.


Photoprotection against nitrogen mustard inactivation in Escherichia coli B.


Evolution of mammals on southern continents. VII. Comparisons of the contemporary mammalian faunas of the southern continents.


Ultrastructure of Brunner's gland cells of the dog. An electron microscopic study on the cellular forms and organization in refed, cholinergically stimulated and fasted animals.


Mrs. Johnstone pioneers.

Mrs. Johnstone pioneers.


Human growth hormone and hypopituitargrowth retardation.


Localization of plasma proteins in kidneys of children with diffuse nephropathies, studied by an indirect immunofluorescent technique.


Gastro-intestinal function after abdominal cobalt irradiation.


Dip stick determination of bacteriuria.

Dip stick determination of bacteriuria.


Prophylactic antibiotics in vaginal hysterectomy.


Oral contraceptive medications and headache.


Effect of chlorothiazide on intravenous glucose tolerance in pregnancy.


Platelet adhesiveness and lipoprotein lipase activity in controls and in subjects taking oral contraceptives.


Pathogenesis of infectious laryngotracheitis virus in chicken kidney cells.


Professor C. Bruce Perry.

Professor C. Bruce Perry.


Rheumatic heart disease: the development of surgical techniques over the past 20 years.


Physicians' preferences in a blind trial of imipramine and amitriptyline.


The effect of lithium carbonate on the electroencephalogram of patients with affective disorders.


Treatment of dysmenorrhoea with dydrogesterone. Results of a double blind trial.


Treatment of malignant superior vena caval obstruction. A randomized study.


Leiomyoma of the ureter. A histologically problematic rare tumor confirmed by immunohistochemical studies.


Primary reticulum cell sarcoma of the skull. Response to irradiation.


Salmonellosis complicating neoplastic diseases.


Nuclear structures of primary malignant lymphoma in the brain.


Studies related to toilet training of the mentally retarded.


The milk-ejection reflex: a neuroendocrine theme in biology, myth and art.


The immunologic nature of kerion celsi formation.


Model experiment on the fixation of lung to study pathomorphology of emphysema complex.


Applying the indirect immunofluorescency test to the study of American cutaneous leishmaniasis.


Dr. Vaman Dinkar Sathaye.

Dr. Vaman Dinkar Sathaye.


Ultrastructure of papain and pepsin digestion fragments of human IgM globulins. The ultrastructure of papain and pepsin-digested products of human IgM globulins has been analyzed. Papain digestion was performed both in the presence and absence of cysteine. The Fcmicro fragment was found to represent the central ring structure in the intact IgM molecule, plus a minor part of the appendages extending from the ring. The Fcmicro ring structure was occasionally seen to be composed of dimers of short rods, probably identical with the endpieces of two micro-chains. Such dimeric structures, released from the intact Fcmicro rings, had a tendency to aggregate sidewise, producing complexes of varying size. The dimensions of the Fcmicro fragments were: outer diameter approximately 85 A, inner diameter about 40 A. The length of the protrusions varied from 20-30 A. The Fabmicro preparations contained long strands of sidewise aggregated, short rod-shaped fragments. No aggregates were seen in the F(ab'')(2)micro preparations. The two Fab''micro units in the dimeric F(ab'')(2)micro fragments were usually parallel to each other. The dimensions of the Fabmicro and F(ab'')(2)micro fragments were 50-80 A x 30 A and 75-80 A x 55 A, respectively. These findings provide morphological evidence that the C-terminal ends of the micro-chains (the Fcmicro fragment) make up the central ring structure in the IgM molecule. They further indicate that the F(ab'')(2)micro fragments constitute about (3/4) of the appendages extending from this ring structure.


Detachment of ribosomal proteins by salt. II. Some properties of protein-deficient particles formed by the detachment of ribosomal proteins.


Detachment of ribosomal proteins by salt. I. Effect of conditions on the amount of protein detached.


Single-strand breaks in bacterial DNA associated with thymine starvation.


Platyamoeba stenopodia n. g., n. sp., a freshwater amoeba.


Cell-free synthesis of bacteriophage f2 maturation protein.


Structure of a nonpathogenic histomonad from the cecum of galliform birds and revision of the trichomonad family Monocercomonadidae Kirby.


American urology--1969. Its achievements--its challenges.


Successful 24-hour canine kidney preservation.


Renal emphysema.

Renal emphysema.


Ureteral reflux in transureteroureterostomy.


A practical method of office bacteriology.


Arteries in canine cardiac homografts. Ultrastructure during acute rejection.


Informed consent and expressed warranty.


Distinctive sulphydryl responses in x-irradiated and in heat-treated Micrococcus radiodurans.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 45-1969.


L-asparaginase: therapeutic and toxic effects in patients with neoplastic disease.


Presentation of Howland Award to Allan M. Butler.


Dermal grafts. Clinical experience in head and neck surgery.


The use and limitations of isotope studies in clinical shock.


Is there a place for proteinase inhibition? A review of the literature.


Palatine tonsils and rheumatoid arthritis. Histopathological, histochemical and immunohistochemical investigations.


Intracellular distribution of lipolytic enzymes in the rat pancreas.


Cellular immunity against tumor antigens.


Multiparameter evaluation of internal mammary artery implant function. An experimental study.


Tracheal compression by the innominate artery in children.


General anesthesia for bronchoscopy. The use of the Sanders bronchoscopic attachment.


Prevention of sternotomy wound disruptions by use of figure-of-eight pericostal sutures.


Variations in the average lifetime of bacterial messenger ribonucleic acid with growth medium and temperature.


The interaction of aflatoxin B1 with polynucleotides and its effect on ribonucleic acid polymerase. 1. The interaction of aflatoxin B(1) with different polynucleotides was studied spectrophotometrically. Equations were derived that enable the degree of binding to be determined without first determining the extinction coefficient of the bound form. 2. The interaction with calf thymus DNA obeys first-order relationships with an association constant of 0.40mm(-1), but there is some evidence for a secondary binding process from results obtained at 390nm. 3. The spectral shifts decreased in the order polyadenylic acid+polyuridylic acid>DNA>polyadenylic acid>polyadenylic acid+polyinosinic acid. Polycytidylic acid, polyuridylic acid, polyinosinic acid (both single- and triple-stranded), AMP, CMP, GMP and UMP did not interact with aflatoxin. It was concluded that there is a requirement for the amino group of adenine (or possibly guanine) for binding of aflatoxin to polynucleotides to occur. 4. Binding is reversed by increasing ionic strength, and by Mn(2+) and Mg(2+) in the concentration range studied (0-5mm). The effect of the Mn(2+) or Mg(2+) was far greater than would be expected on the basis of their ionic strength. With both the bivalent cations and sodium chloride the reversal is greatest with double-stranded polynucleotides. 5. Inhibition in vitro of the DNA-dependent RNA polymerase of Escherichia coli by aflatoxin B(1) was detected only in the absence of Mg(2+) and at concentrations of Mn(2+) below the optimum for RNA synthesis in vitro. 6. The degree of inhibition (maximally 30%) was dependent on the concentration of Mn(2+) and decreased during incubation.


Frances E. Fischer, President--the American Dietetic Association.


Biographical notes from the history of nutrition. Louis Sigurd Fridercia--February 24, 1881-February 26, 1947.


Influence of certain drugs on cataract formation.


Kinship of Smenkhkare and Tutankhamen affirmed by serological micromethod. Microdetermination of blood groupsubstances in ancient human tissue.


Kinship of Smenkhkare and Tutankhamen demonstrated serologically.


Polarity and the degradation of mRNA.

Polarity and the degradation of mRNA.


Initiator protein dependent binding of messenger RNA to ribosomes.


Anencephaly and spina bifida: a possible example of cytoplasmic inheritance in man.


Bacterial iron metabolism and immunity to Pasteurella septica and Escherichia coli.


The changing picture of asthma.

The changing picture of asthma.


Current bibliography on allergy and applied immunology.


A microscopic method for the evaluation of the bacillary output in sputum of tuberculous patients under chemotherapy.


Ultrasound in heart transplants.

Ultrasound in heart transplants.


Nitroheterocycles with antiparasitic effects.


Initial experiences with niridazole.

Initial experiences with niridazole.


Clinical evaluation of niridazole in Schistosoma haematobium and mansoni infections.


Symposium: a sesquicentennial study of Daniel Drake and the results of his theories on medical education. Opening remarks.


Symposium: A sesquicentennial study of Daniel Drake and the results of his theories on medical education. Aaron's rod.


Symposium: A sesquicentennial study of Daniel Drake and the results of his theories on medical education. The good seed.


Cardiac transplantation in man. IV. Early results.


Erythropoietin activity in anephric or renal allotransplanted man.


Factors influencing survival after human heart transplantation.


Antibodies and human transplant rejection.


Mechanisms for the high circulatory requirements in sepsis and septic shock.


New Congress president.

New Congress president.


Chromatography of alkylating agents. II. Nitrosamines, epoxides, lactones, methanesulfonates and miscellaneous derivatives.


Resistance of Salmonella typhimurium mutants to galactose death. A class of galactose-resistant mutants has been derived from strains of Salmonella typhimurium which are defective in uridine diphosphoglucose-4-epimerase. Resistant strains are phenotypically similar to parent organisms but do not lyse in the presence of galactose. Low levels of functional epimerase can be detected in induced cells grown at 20 C but not at 37 C, and acid is not produced from galactose. Sufficient galactose is synthesized at reduced temperatures to fabricate smooth lipopolysaccharide and acceptor sites for phage P22 from galactose-deficient media. The leaky nature of these mutants may account for resistance to galactose death by maintaining galactose metabolites at a subcritical level. Glucose protects sensitive strains by control of levels of toxic metabolites by catabolite repression.


R factors improving survival of Escherichia coli K-12 after ultraviolet irradiation. Two R factors which have the capacity to improve survival of some strains of Escherichia coli K-12 by approximately 60% after ultraviolet light have been identified and characterized. Both are fi(-), but neither produce colicins. The ability to enhance survival can be separated from all other identified R-factor functions. Improved survival does not result from improved excisional capacity, but does require an intact host capacity for genetic recombination. No effects on host cell growth or postirradiation lag were observed. A proposed mechanism of action is described.


Isolation and partial characterization of an immunogenic moiety obtained from Salmonella typhimurium. Ribosomal preparations obtained from Salmonella typhimurium by differential centrifugation and sodium dodecyl sulfate (SDS) treatment of the bacillary lysate were found to be immunogenic in F(1) hybrid (C(3)H/HeJ x DBA/2J) and albino Swiss mice, as determined by progressive host survival. The immunity obtained was independent of the need for adjuvant and dependent on the dosage of immunogen given. Immunizations with the ribosomal preparations induced an immune response comparable to that obtained by vaccination with living organisms and significantly greater than that obtained by immunization with heat-killed salmonellae, purified lipopolysaccharide, or crude and SDS-treated endotoxin preparations. No effect on the immunogenicity of the ribosomal fraction was observed by enzymatic treatment with trypsin, Pronase, deoxyribonuclease, and pancreatic ribonuclease. Linear sucrose density gradient resolution of the preparations showed that the immunogenicity of the ribosomal fraction was not unique to any one of its subcomponents. Ethyl alcohol-precipitated, crude ribonucleic acid preparations obtained from the ribosomal and sucrose density-resolved ribosomal preparations were found to induce an immune response comparable to that obtained by immunization with the entire ribosomal fraction. Dialysis in doubly distilled demineralized water slightly reduced the immunogenicity of the preparation; however, comparable dialysis in 10(-4)m MgCl(2)-phosphate buffer did not. Chemical assays of the preparations found to be immunogenic were performed.


Pseudocompact-type growth and conversion of growth types of strains of Staphylococcus aureus in vitro and in vivo. Some strains of Staphylococcus aureus grew as compact colonies in Brain Heart Infusion-serum-soft agar but as diffuse colonies in a modified Staphylococcus 110-serum-soft agar. These strains were designated "pseudocompact." Strains showing compact-type colonial morphology in both media were designated "compact," whereas strains showing diffuse-type growth in both media were designated "diffuse." It was observed that the most recently isolated strains of S. aureus were of the pseudocompact type, whereas most stock culture strains tested were of the compact type. Using cultures recently isolated from clinical material, it was shown that pseudocompact strains convert to compact-type growth after prolonged incubation. Interconversion of compact, diffuse, and pseudocompact growth forms could be induced in vitro by appropriate cultural conditions, and conversion of growth type was also observed in vivo. Femoral abscesses produced in mice by four different compact-type strains showed conversion to diffuse or pseudocompact-type growth during the course of the infection.


Mutants of Escherichia coli with an altered tyrosyl-transfer ribonucleic acid synthetase. We have isolated several mutants defective in the gene for tyrosyl-transfer ribonucleic acid (tRNA) synthetase (tyrS). One of these mutants is described in detail. It was isolated as a tyrosine auxotroph with defects both in the tyrosyl-tRNA synthetase and in the tyrosine biosynthetic enzyme, prephenate dehydrogenase. It also had derepressed levels of the tyrosine-specific 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase. The latter finding suggested that a wild-type tyrS gene was required for repression of the tyrosine biosynthetic enzymes. The following results demonstrated that this hypothesis was not correct. (i) When the defective tyrS gene was transferred to another strain, the tyrosine-specific DAHP synthetase in that strain was not derepressed, and (ii) two other mutants with defective tyrosyl-tRNA synthetases had repressed levels of the tyrosine biosynthetic enzymes. The tyrS gene was located near minute 32 on the Escherichia coli chromosome by interrupted mating experiments.


Compounds which serve as the sole source of carbon or nitrogen for Salmonella typhimurium LT-2. About 600 compounds were screened as possible carbon or nitrogen sources for Salmonella typhimurium LT-2. About 100 utilizable compounds were found.


Inducible synthesis of beta-galactosidase in disrupted spheroplast of Escherichia coli. A membrane preparation obtained from osmotic lysate of spheroplasts of Escherichia coli cells showed an activity of synthesizing beta-galactosidase which was dependent upon oxidative phosphorylation. The synthesis was inhibited by the addition of actinomycin D or of chloramphenicol. The beta-galactosidase synthesized in the membrane preparation was completely released into the medium, while that synthesized in the spheroplasts and intact cells remained within the cells. The minimum concentration of the inducer, methyl-beta-d-thiogalactoside, required for the induction of beta-galactosidase was 5 x 10(-5)m for intact cells, 3 x 10(-4)m for spheroplasts and 1 x 10(-3)m for membrane preparation. Incorporation of labeled glucose into insoluble components in membrane preparation was extremely low compared with that in intact cells or in spheroplasts. Based on these and other observations, the nature of this membrane preparation is discussed in relation to the structure of E. coli cells.


Effect of bile acids on the intestinal absorption of endotoxin in rats. The absorption of tritium-labeled Escherichia coli O89 Westphal-type endotoxin from the peritoneal cavity of rats was diminished by bile by 23% and by sodium deoxycholate by 47%, respectively. Practically, there is no endotoxin absorption from the intestinal tract of normal rats. The bile duct of rats was chronically cannulated for experimental purposes. A significant amount of perorally administered endotoxin absorbed from the intestinal canal into the blood in the rats treated thus. Absorption was demonstrated by the lethal effect of endotoxin on rats previously hypersensitized by lead acetate, and by the radioactivities found in the blood samples. The intestinal absorption of endotoxin in rats, rendered bile-deficient, may be prevented by sodium deoxycholate. Supported by their experimental findings, we emphasize the important role of bile acids in the defense mechanism of the macroorganism against bacterial endotoxins.


Recognition of altered deoxyribonucleic acid in recombination. Kinetics of inactivation of transduction by phage P1bt which had been treated with ultraviolet light (UV) or nitrous acid (NA) was examined. With Escherichia coli B/r (radiation-resistant), low doses of UV increased transduction frequency, but the frequency was exponentially inactivated by higher doses. Little initial stimulus was observed in strain B(s-1) (radiation-sensitive). The final rate of decay was the same as in B/r. The initial stimulus of transduction in B/r was probably a consequence of increased recombination resulting from dark repair. It was estimated that another nucleotide within 1000 nucleotide pairs had to be damaged by UV to prevent a given nucleotide from successful transduction. The NA dose response was the same for the two strains. An initial stimulus of transduction was followed by exponential decline. The UV-repair enzymes missing in B(s-1) were not required for repair of NA-induced damage to transducing or lytic phage DNA. Low recovery of new mutations in the transductants showed that mutagen-induced damage to transducing DNA was excluded from recombinant chromosomes. The few recovered mutants may have resulted from "normal" error in recombination.


Transfer of infectious drug resistance in microbially defined mice. Germ-free mice were intentionally associated with drug-resistant donor strains of Escherichia coli known to carry R factors and with drug-sensitive recipient strains. In vivo transfer of R factors was observed in all experiments, involving five different donor-recipient combinations. The number of converted recipients varied, depending upon the donor-recipient combination, but in all cases it was restricted by limiting numbers of either recipient or donor strains in the digestive tract of the microbially defined mice. Converted recipients were detected in fecal material as early as 5.5 hr after mice were associated with donor and recipient bacteria. Donors, recipients, and converted recipients were detectable in the stomach, small intestine, cecum, and large intestine of the microbially defined mice and their suckling young.


Characteristics of some multiply recombination-deficient strains of Escherichia coli. Strains of Escherichia coli have been made carrying lesions in more than one gene determining recombination. The following genotypes were constructed and verified: recC22 recB21 recA(+), recC22 recB21 recA13, recC22 recB(+)recA13, and recC(+)recB21 recA13. All multiple rec(-) strains carrying recA13 were similar to AB2463, which carries recA13 alone, in their UV sensitivities, recombination deficiencies, and inabilities to induce lambda phage in a lysogen. However, whereas AB2463 shows a high rate of ultraviolet (UV)-induced deoxyribonucleic acid (DNA) breakdown, the multiple rec(-) strains showed the low level characteristic of strains carrying recC22 or recB21 alone. The strain carrying both recC22 and recB21 was similar in all properties to the single mutants, suggesting that both gene products act in the same part of the recombination and UV repair pathways. It is concluded that in a Rec(+) strain, the recA(+) product acts to inhibit DNA breakdown determined by the recC(+) and recB(+) products.


Suppression of amber and ochre mutants in Salmonella typhimurium by a mutant F'-1-gal factor carrying an ochre suppressor gene. A Salmonella typhimurium strain was given the amber mutation hisC527 by transduction, made galactose-negative by mutation, then infected with the F'-1-gal factor. Of 107 spontaneous and mutagen-induced histidine-independent mutants tested, 3 proved to result from suppressor mutations within the F' factor. The mutant F' factors, when transferred to S. typhimurium and E. coli auxotrophs, suppressed amber and ochre but not UGA or missense mutants, and are inferred to carry ochre suppressor genes. Attempts to isolate an F' amber suppressor mutant were unsuccessful. A suppressor F' factor was transferred to 14 rough mutants which had been isolated from LT2 hisC527 (amber) by selection for resistance to phage P22.c2. One rough mutant was partly suppressed, as shown by its acquisition of O agglutinability and by alterations in its phage resistance pattern. Phage P22h grown on the suppressed mutant contransduced its rf. gene with cysE(+) and with pyrE(+), and the affected locus is inferred to be rfaL. Both the original and the mutant F' factors conferred resistance to the rough-specific phage Br60, which is therefore "female-specific."


Control of cell division in Escherichia coli: experiments with thymine starvation. This paper describes the kinetics of cell division in populations of cells which have been grown first under conditions which specifically inhibit deoxyribonucleic acid (DNA) synthesis (in the absence of thymine or the presence of nalidixic acid) and subsequently under conditions which allow DNA synthesis to recommence. Cell division does not take place during inhibition of DNA synthesis. There is a delay between recommencement of DNA synthesis and recommencement of cell division. The length of this delay increases as a function of the length of the preceding period of inhibition of DNA synthesis. The first division after this delay is partly synchronous, but all subsequent division is asynchronous. These observations are explained in terms of a model which supposes that the formation of initiator of chromosome replication during a period when DNA synthesis is inhibited results in a block to cell division. Division does not then occur until this "extra" round of DNA synthesis is completed.


Evolution of a site of specific genetic homology on the chromosome of Escherichia coli. Integration of the factors F(v) and F into the chromosome of a substrain of Escherichia coli K-12 has been studied. The F(v) factor is a fertility factor derived from Col V, lacking the ability to govern the production of colicin V. The derivatives of an Hfr(v) (Hfr isolated from a V colicinogenic parent) strain, PK2 (initially isolated from C600 V(+)), were shown to retain a unique bidirectional sex factor affinity locus between recA and pheA. This site shows no affinity for the E. coli K-12 F factor as shown by inability to isolate Hfr strains with origins in this region from a parental strain containing a cytoplasmic F factor. However this area exhibits two regions of homology to the V colicinogenic factor. One gives rise to Hfr(v) strains identical to the original Hfr(v) strain, PK2, with an origin and polarity of transfer designated pheA-CC injecting markers in the order pheA-his-trp-pro. The second gives rise to strains apparently originating at the same site but with reverse polarity designated recA-C, transferring markers in the order recA-thyA-str-xyl. For strains possessing the F(v) factor only the second homology is apparent. A model for the evolution of these strains is presented.


Characterization of pyridoxine auxotrophs of Escherichia coli: chromosomal position of linkage group I. The chromosomal location of Group I pyridoxine mutations in Escherichia coli is shown to be adjacent to dsdA,aroC, and purF (old purC) in E. coli B x K-12 hybrids. All mutants previously classified into Group I by nutrition tests and transduction frequency tests are shown to be linked to dsdA.


Kinetics of adsorption of colicin CA42-E2 and reversal of its bactericidal activity. The kinetics of killing of Escherichia coli K-12 by colicin CA42-E2 have been studied, and the data were used to estimate the adsorption constant of this colicin under various environmental conditions. Evidence was obtained suggesting that the adsorption of colicin occurred in two stages; the earlier stage was reversible and did not lead to the death of the cell, the latter stage was irreversible and bactericidal. Cells which had adsorbed a lethal quantity of colicin could be rescued for a short time by inactivating the adsorbed colicin with trypsin. However, when the metabolic activity of the cells was totally arrested the lethal effect of adsorbed colicin was subject to trypsin reversal over long periods of time.


Temperature-sensitive conjugation-defective F factor in Escherichia coli. A mutant strain of Escherichia coli K-12 which is unable to transfer genetic material at 42 C has been isolated. Although transfer is inhibited at this temperature, the formation of specific pairs is unaffected. This strain and its derivatives also show an altered sensitivity to certain ribonucleic acid-containing male specific phages at 42 C. Both phenotypic changes are reversed when a derepressed fi(+) R factor is present in the same cell and a revertant which has regained both activities has been obtained. The nature of the temperature-sensitive defect and its effect on the conjugation process are discussed.


Sodium-stimulated transport of glutamate in Escherichia coli. Wild-type Escherichia coli B grew poorly on glutamate as the sole carbon source, except at very high concentrations of the amino acid. The addition of sodium ion markedly stimulated the growth. It had the same effect in a mutant of E. coli B selected for the ability to grow at low glutamate concentrations. Sodium ion also potentiated growth inhibition by analogues of glutamate. The uptake of glutamate by nongrowing cells of the mutant was markedly stimulated by sodium ion in the presence of an energy source, chloramphenicol, and arsenite, which retarded glutamate degradation.


Effect of R factors and other plasmids on ultraviolet susceptibility and host cell reactivation property of Escherichia coli. Some R factors, like some colicin factors, confer partial protection against the bactericidal effect of ultraviolet (UV) irradiation. Of 31 plasmids (17 R, 3 col, and 11 R-col factors) tested in Escherichia coli K-12, 15 protected, 11 had little or no effect, and 5 caused increased UV susceptibility. The effect of representative plasmids was qualitatively the same in K-12 of wild-type UV sensitivity, lambda-lysogenic or non-lysogenic, and in UV-sensitive mutants of classes uvrA, uvrB, uvrC, and recA (except that a sensitizing factor did not increase the sensitivity of two recA hosts). It is inferred that the UV-protecting effect of some plasmids does not result from their specifying enzymes similar to those deficient in such mutants. UV killing of multiply auxotrophic K-12, of wild-type sensitivity or recA or uvrC, was reduced by deprivation of required amino acids for 2 hr before irradiation, and further reduced if "starvation" was continued for 2 hr after irradiation. The plasmids tested in these conditions produced qualitatively the same effects as in nonstarved cells-except that in K-12 of wild-type UV sensitivity the effect of protecting plasmids was reversed (i.e. they caused decreased survival) when the cells were starved after irradiation. Two UV-protecting R factors reduced the ability of HCR(+) K-12 to support growth of irradiated phage T1.


Nature of lactose-fermenting Salmonella strains obtained from clinical sources. Six of seven lactose-fermenting (lac(+)) Salmonella strains obtained from clinical sources were found to be capable of transferring the lac(+) property by conjugation to Salmonella typhosa WR4204. All of the six S. typhosa strains which received the lac(+) property transferred it in turn to S. typhimurium WR5000 at the high frequencies typical of extrachromosomal F-merogenotes. These six lac elements were also transmissible from S. typhosa WR4204 to Proteus mirabilis and to some strains of Escherichia coli K-12; moreover, they were capable of promoting low frequency transfer of chromosomal genes from S. typhimurium WR5000 to S. typhosa WR4204. One of these lac elements was shown also to be capable of promoting low frequency chromosome transfer in E. coli K-12. E. coli K-12 strains harboring these lac elements exhibited sensitivity to the male specific phage R-17. Sensitivity to R-17 was not detected in Salmonella strains containing the elements. Examination of the lac elements in P. mirabilis by cesium chloride density gradient centrifugation showed that each element had a guanine plus cytosine content of 50%. The sizes of the elements varied from 0.8 to 3% of the total Proteus deoxyribonucleic acid. The amount of beta-galactosidase produced by induced and uninduced cultures of S. typhimurium WR5000 and S. typhosa WR4204 containing the lac elements was lower than that produced by these strains with the F-lac episome. The heat sensitivity of beta-galactosidase produced by the lac elements in their original Salmonella hosts indicated that the enzyme made by these strains differs from E. coli beta-galactosidase.


New methionine structural gene in Salmonella typhimurium. Eight metH mutants in Salmonella typhimurium with closely linked sites of mutation which could grow only on methionine were isolated from a metE mutant deficient in N(5)-methyltetrahydropteroyltriglutamate-homocysteine transmethylase; their deficiency in cobalamin-dependent N(5)-methyltetrahydrofolate-homocysteine transmethylase was supported by the results of enzyme studies of one of them. Cotransduction of metH and metA (homoserine O-transsuccinylase) mutants was obtained, thus revealing linkage between a second pair of the six known methionine structural genes. One metH mutant clearly differed from the rest in that it reverted at a higher frequency, was temperature sensitive, complemented all other metH mutants, and was located farthest from the metA gene.


Mutator gene studies in Escherichia coli. An Escherichia coli mutator gene, mutT, has been shown by P1-mediated crosses to map between the leucine and azide loci. The mutT1 and azi-r alleles cotransduce with a frequency of >92%. In mutT1/mutT(+) merodiploids, the mutT1 phenotype is recessive; in mutT1/F'trp or mutT1/F'lac merodiploids, the mutT1 allele has a trans effect. The gene can mutate lambda and T7 phage but not T1, T3, T4, T5, and S13.


L-arabinose binding protein from Escherichia coli B-r. A protein which is capable of binding l-arabinose-1-(14)C has been isolated from l-arabinose-induced cultures of Escherichia coli B/r. Analysis for this l-arabinose-binding protein (ABP) in a number of l-arabinose-negative mutants suggests that the ABP is not coded for by any of the known genetic units of the l-arabinose complex yet is under the control of the regulator gene araC. The ABP has been purified and found to bind l-arabinose, d-fucose, d-xylose, and l-ribulose with decreasing affinities. The K(m) for l-arabinose is 5.7 x 10(-6)m. The molecular weight, as determined by equilibrium centrifugation, was found to be 32,000. The protein was observed to have many features that liken it to other recently isolated binding proteins that have been implicated in the active transport of small molecules.


Externally suppressible frameshift mutant of Salmonella typhimurium. Prototrophic revertants of ICR-191A-induced frameshift mutant hisD3018 have been induced spontaneously by ICR-191A and N-methyl-N'-nitro-N-nitrosoguanidine (NG) treatment. In each case two genetically distinct prototroph classes were differentiated by transducibility into his deletion recipients: (i) transducible, generally fast-growing revertants within the hisD gene producing from 10 to 100% of normal amounts of histidinol dehydrogenase and (ii) nontransducible slow-growing prototrophs with very low levels of enzyme activity of which at least some arose by external suppression. These nontransducible revertants, whether arising spontaneously or in the presence of ICR-191A or NG, contain histidinol dehydrogenase which is electrophoretically similar to the wild-type enzyme.


Nature of the hisD3018 frameshift mutation in Salmonella typhimurium. Histidinol dehydrogenase from three differing revertants of ICR-191A-induced frameshift hisD3018 has been purified and examined for amino acid replacements. The enzyme from one spontaneously arising revertant, R7, contains an extra proline residue, whereas that from another, R5, contains an extensive frameshifted sequence, four amino acid residues of which have been identified to date. The amino acid replacement data are in agreement with the in vitro code word assignments and allow the characterization of the hisD3018 frameshift as an addition of one nucleotide pair, most likely guanine plus cytosine. Enzymatic data for those ICR-191A-induced revertants of hisD3018 arising within the hisD gene indicate that the enzyme is wild type and, therefore, that ICR-191A can cause deletions as well as additions of single base pairs. The wild-type amino acid sequence is restored in enzyme from an N-methyl-N'-nitro-N-nitrosoguanidine (NG)-induced revertant, R29, suggesting that NG is a base-deleting as well as a base-substituting mutagen. The unusual response of hisD3018 to external suppressors is considered in terms of reinitiation of protein synthesis out of phase, coupled with suppression of a nonpermissive missense codon so generated, and of an alternative hypothesis invoking a true frameshift suppressor transfer ribonucleic acid with an extended or deleted anticodon.


Electron microscopic studies on opaque colony variants of group A streptococci. Opaque colony variants of two strains of group A streptococci have been compared with blue colonies of the same strains by electron microscopy. In opaque colonies, the cocci are joined into elongated chains by exaggerated intercellular septa that often occupy the major portion of each cell's circumference. The thickness and lamination of cell walls in opaque colony variants are identical to those aspects of cell walls in blue colony forms. The similarity in cell wall architecture is found between opaque and blue forms whether or not M protein (and M associated surface fimbriae) is present. Extensive, direct contact between the nucleoid and the cytoplasmic membrane beneath intercellular septa is seen in opaque colony variants. The relationship of this marked nucleoid-cytoplasmic membrane association to the unusual chain forms in the opaque colony variants is unclear.


Biosynthesis of cell wall lipopolysaccharide in mutants of Salmonella. V. A mutant of Salmonella typhimurium defective in the synthesis of cytidine diphosphoabequose. A mutant of Salmonella typhimurium LT2 was found to be unable to convert cytidine diphospho-4-keto-6-deoxy-d-glucose into cytidine diphosphoabequose. The mutation maps in the rfb gene cluster, which is known to be involved in the biosynthesis of the peripheral, "O side-chain" portion of cell wall lipopolysaccharide. In spite of the fact that, in the O side chains, abequose is not a part of the main chain but occurs as short branches, the mutant appears to be unable to polymerize oligosaccharide "repeat units" into long O side chains. The following evidence indicates that this failure is the result of the absence of cytidine diphosphoabequose rather than that of a superimposed second mutation in other genes of the rfb cluster. (i) The mutant does not behave like a multisite mutant in genetic crosses, and it gives rise, at a high frequency, to "revertants" where the ability to synthesize cytidine diphosphoabequose and the ability to synthesize normal lipopolysaccharide with O side chains are both restored. (ii) The mutant strain has normal levels of activity of all of the other enzymes known to be involved in O side-chain synthesis, except that the levels of several enzymes were lowered by about 30% owing to the polarity effect of the mutation. That the lowering of these enzymes is not responsible for the failure of the mutant to synthesize O side chains is clear from the fact that there were revertants which had regained some ability to synthesize abequose but still had lowered levels of these other enzymes, and that this type of revertant produced lipopolysaccharide with considerable amounts of O side chains.


Pituitary activation by bacterial endotoxins in rainbow trout (Salmo gairdneri). Endotoxins from Escherichia coli and Aeromonas salmonicida caused marked cortisol production in the rainbow trout (Salmo gairdneri).


Restriction in genetic crosses between Escherichia coli and Shigella flexneri. Shigella flexneri restricts Escherichia coli deoxyribonucleic acid (DNA) and can modify phage DNA so that it is restricted in E. coli.


Effect of endotoxin on the serum ribonuclease activity in rats. The effects of endotoxin shock, endotoxin tolerance, and lead acetate plus a minute amount of endotoxin on the serum ribonuclease activity of rats was measured. Changes in serum ribonuclease activity after various entoxin treatments could be a primary effect or a secondary effect of the damaging effect of endotoxin.


The initiation of T4 deoxyribonucleic acid-dependent beta-glucosyltransferase synthesis in vitro.


Mechanism of tuberculostasis in mammalian serum. II. Induction of serum tuberculostasis in guinea pigs. The growth of tubercle bacilli in serum samples of untreated animals depends upon the availability of ionic iron which serves as a growth factor in supporting bacillary multiplication. The amount of available iron in serum is determined by the ratio between iron-saturated and iron-free transferrin; a low value for the ratio is associated with tuberculostasis (e.g., human serum, 0.4), whereas a high value is associated with the growth-supporting quality (e.g., guinea pig serum, 5.6). The treatment of guinea pigs with lipopolysaccharide of Escherichia coli or tuberculous cell wall material consistently and significantly reduced serum iron levels; a similar but less striking effect was observed in BCG-vaccinated animals. Pronounced differences were observed in the time of appearance and duration of serum hypoferremia; in lipopolysaccharide-treated animals, it appeared in 1 day and lasted for several days, whereas in BCG-vaccinated animals it appeared in about 2 weeks and lasted for much longer time periods. The induced hypoferremia was always associated with the concomitant development of serum tuberculostasis which could be neutralized by the addition of iron. These results indicate, therefore, that the mechanism of induced serum tuberculostasis in lipopolysaccharide- or tuberculous cell wall-treated and BCG-vaccinated guinea pigs is the same as that present in tuberculostatic sera of untreated animals.


Dynamics of uptake of estrogens and androgens by human endometrium. Application of a double isotope perfusion technique.


An extracellular nuclease from Serratia marcescens. I. Purification and some properties of the enzyme.


Induction and repression by arginine in Escherichia coli. Different mechanisms.


Arginine decarboxylase from Escherichia coli. 3. Subunit structure.


Purification and characterization of Thymidine 5-monophosphate kinase from Escherichia coli B.


Genetic control of histidine degradation in Salmonella typhimurium, strain LT-2.


Genetic and metabolic control of histidase and urocanase in Salmonella typhimurium, strain 15-59.


Intranuclear rods in pancreatic islet beta-cells.


Aortic arch atresia and interreuption of the aortic arch. Experience in 11 cases of operation.


Determination of the accuracies of the dye-dilution and electromagnetic flowmeter methods of measuring blood flow.


The law relating to organ transplants.

The law relating to organ transplants.


Ergonomic design of a needleholder.

Ergonomic design of a needleholder.


The importance of spleen scintigraphy in malignant lymphomas.


Principal biochemical characteristics of the EC-2 L-asparaginase from Escherichia coli AT.


Experimental and clinical cardiac transplantation.


Multiple organ transplantation from a single donor. Liver, heart, kidneys, corneas.


Operable forms of heart disease. Introduction.


Growth and transformation of phage T4 in Escherichia coli B-4, Salmonella, Aerobacter, Proteus, and Serratia.


Discrete buoyant density distribution among RNA phages.


Heterotransplantation of aortic valves.

Heterotransplantation of aortic valves.


Pulmonary surfactants and their role in lung disease.


Intestinal malabsorption in infancy and childhood.


Atrial septostomy in congenital heart disease.


Chronic granulomatous disease of childhood.


Hypernatremic dehydration.

Hypernatremic dehydration.


Transient diabetes of the newborn.

Transient diabetes of the newborn.


The management of bleeding in hemophilia.


Bilirubin binding and kernicterus.

Bilirubin binding and kernicterus.


Alcohol and tobacco amblyopia: a historical survey. XXXI DeSchweinitz lecture.


Ocular tolerance of corticosteroid formulations.


William Peacey Shepard--1895-1969.

William Peacey Shepard--1895-1969.


The health administration establishment: underachiever.


A selected public health bibliography with annotations.


In memoriam Corneille J. F. Heymans. Nobel Prize 1938 for Physiology and Medicine.


Medical treatment of occlusive arterial disease of the legs. Walking exercise and medically induced hypertension.


Induction of antibodies in vitro.

Induction of antibodies in vitro.


Sequences in medical microbiology. Some observations over fifty years.


New approaches to bacterial taxonomy: perspective and prospects.


Rickettsiae (as organisms).

Rickettsiae (as organisms).


Structure, physiology, and biochemistry of the chryophyceae.


Bacillus thuringiensis: microbiological considerations.


Kinetics of nutrient-limited growth.

Kinetics of nutrient-limited growth.


Ultraviolet-induced mutation and DNA repair.


Total synthesis of acetate from CO2 by heterotrophic bacteria.


Sturge-Weber syndrome.

Sturge-Weber syndrome.


Bacterial conjugation.

Bacterial conjugation.


The epidemiology of epithelioma of the mouth and tongue.


A Golgi study of the desending medial forebrain bundle.


Fixation of the central nervous system by perfusion of the cerebral ventricles with a threefold aldehyde mixture.


A new approach to the microbial drug resistance problem.


Bacterial meningitis among newborn infants.


Non-transferable tetracycline resistance in Escherichia coli K 12.


Management of neonatal bacterial infections. Rx: continual alertness, specific treatment, and ampicillin with kanamycin until the culture reports are back.


Various applications of endosseous implants: report of 5 cases.


Analysis of allogeneic inhibition of the colony-forming capacity of bone marrow cells.


Utilization of search tools for compiling a bibliography on parenteral dosage forms.


Method of correction when determining cell concentrations by the plating technique.


Radiophotoluminescence dosimetry bibliography II.


Destination Lagos. Teaching dentistry in West Africa.


A clinical investigation of oxazepam as an adjunct for periodontal therapy.


Autogenous red marrow grafts: their potential for induction of osteogenesis.


Antidermatophytic properties of griseofulvin derivatives with potential systemic insect-repellent activity.


Gastroenteritis and maternity hospitals.


Pharmaceutical applications of polymorphism.


N-tuplets in computer diagnosis.

N-tuplets in computer diagnosis.


High blood pressure--ancient, modern and natural.


LSD and chromosomes. A controlled experiment.


Theophile Bonet (1620-1689), physician of Geneva.


Daniel Lambert, the great.

Daniel Lambert, the great.


Effects of anorexiants on plasma lipids and other physiological parameters in man.


Single dose-effect relationships of lithium on the electrical activity of the cerebral cortex and of the heart.


Teaching for personal growth: an introduction to new materials.


New challenges for clinical pharmacology.


The utility of projective techniques and the function of the clinical psychologist.


Importance of initial pain in post-operative assessment of analgesic drugs.


Controlled comparison of SK&F 14336 and chlorpromazine in newly admitted schizophrenics.


Prostaglandins.

Prostaglandins.


The story of the First Dental History Society.


Early dental history.

Early dental history.


Highlights in the dental history of the State of New York.


A history of dental libraries in the United States.


Inhibition of mammalian protein synthesis by antibiotics.


Lattice or Reis-Bücklers corneal dystrophy: a question of stromal pathology.


Management of chronic corneal ulcers.

Management of chronic corneal ulcers.


Modifications in lower extremity bracing for specific disability problems.


Postoperative pulmonary complications.

Postoperative pulmonary complications.


Posterior bone grafts in nonunion of fractures of the shafts of the tibia: a review of 27 cases.


Cervical dysplasia associated with azathioprine (Imuran) therapy.


Hair spray: an inexpensive aerosol fixative for cytodiagnosis.


Activation analysis of paper chromatograms.


Effects of vitamin D in the human.

Effects of vitamin D in the human.


Studies on the mode of action of vitamin D.


Ascorbic acid and the biosynthesis of collagen and elastin.


Interactions of transfer RNA from E. coli and methylated bovine serum alumin.


PMR studies of organic solvent denatured transfer RNA.


Allosteric control of biodegradative L-threonine dehydrase: effect of AMP on an early stp in the reaction mechanism.


Light and electron microscopy of virus-associated, intranuclear paracrystals in cultured cells infected with types 2,4,6, and 18 human adenoviruses.


A study of 200 cases of acute diarrhoea in adults.


Autoimmunity and breach of the blood-epithelial barrier.


Correlation between gingivitis and orogranulocytic migratory rate.


An experimental method of studying blood flow in canine bipedicle tube flaps.


The internal surface and pore structure of enamel.


Demonstration of intranuclear T-antigen by antisera to purified adenovirus 7.


Minimal effective dosages in mice of clofazimine (B,663) and of ethionamide against Mycobacterium leprae.


Alterations in pituitary follicle-stimulating hormone (FSH) and hypothalamic FSH-releasing factor (FSH-RF) during puberty.


Establishment of lines from normal human blood leukocytes by co-cultivation with a leukocyte line derived from a leukemic child.


In vitro studies on protein digestion, amino acid absorption interactions.


The hepatic uptake of bacterial endotoxin. I. The influence of humoral and cellular factors.


Effect of pyridoxine deficiency onthe induction of immune tolerance in mice.


A simplified, rapid method for skin transplantation in mice.


Is there a commissure of Gudden in man? An examination of the published evidence, with a note on the medial root of the human optic tract.


Controlled zinc supplementation for malnourished school boys: a pilot experiment.


Social science views of a controversy in science and politics.


Absorption, tissue distribution, endogenous excretion, and homeostatic control of zinc in ruminants.


Matters of gravity.

Matters of gravity.


A case report of bilateral microphthalmos with cysts.


Dr. Gibson E. Craig.

Dr. Gibson E. Craig.


Improvements in oxygen intake capacity resulting from sports and exercise training programs: a review.


Some aspects of muscular movement: A review.


Quantitative studies on the arrangement of human metaphase chromosomes. II. Influence of the preparation technique on the association pattern of the acrocentric chromosomes.


Use of computers in the interpretation of electrocardiograms.


Degenerative changes in the entopeduncular nucleus following lesions of the caudate nucleus: an electron microscopic study.


Recollections of personalities involved in the early history of American biochemistry.


Isotopic dilution analysis. Cholesterol in gallstones and caffein in tea, cola, and NoDoz.


The pathogenesis of dying-back polyneuropathies. I. An ultrastructural study of experimental tri-ortho-cresyl phosphate intoxication in the cat.


The effects of ageing and of cachexia upon skeletal muscle. A histopathological study.


Three-year observation of a myasthenic syndrome concurrent with other autoimmune syndromes in a patient with thymoma.


P. C. P. Cloake (1890-1969).

P. C. P. Cloake (1890-1969).


The influence of stimulation of the hypothalamus and of the dorsomedial nucleus upon experimental bradykinesia and akinesia.


Leonhard Rauwolf, M.D. The physician-botanist honored and reappraised.


Helminthiasis. Trematode infections.

Helminthiasis. Trematode infections.


The prevalence and pattern of enteropathogenic E. coli, shigella and salmonella among infants and young children in the Etimesğut rural area. A study of cases between March and June, 1968.


The foundation and early development of the Royal Belfast Hospital for sick children.


An alternative method of dealing with incontinence in children with neurogenic bladders.


Comparative clinical studies of a new somnifacient combination of methaqualone and etodroxizine versus glutethimide and placebo. Cross-over double-blind control test with sequential analysis.


A double-blind clinical trial of a new sleep-inducing combination of methaqualone and etodroxizine compared with nitrazepam and placebo.


The treatment of the reticuloses.

The treatment of the reticuloses.


One way to think about gene conversion.

One way to think about gene conversion.


Gene amplification in ontogeny and phylogeny of animals.


Extracranial fixation of the facial skeleton: review and report of case.


The surgical treatment of vertigo.

The surgical treatment of vertigo.


A simple turbidity cell for continuously monitoring the growth of bacteria.


An alternate to pollicization in subtotal thumb reconstruction.


The tail of the helix as a key to otoplasty.


Use of mesh skin autografts in the burned patient.


More puka than skin.

More puka than skin.


Successful attempt of reconstruction of a nose from a completely separated piece of skin from the leg, by Prof. Dr. Bünger, Marburg, Germany. (Journal der Chirurgie and Augenheilkunde, 4: 569, 1822). Translated from the German by Dr. Hans May.


Large-mesh skin grafts.

Large-mesh skin grafts.


Theories of the mechanism of action of inhalation anesthetics.


The effect of chain length on the binding of polyuridylic acid to ribosomes.


Climatology and medicine. V.

Climatology and medicine. V.


Gas chromatography: fundamentals and application.


Pyrimidine reversal of emimycin inhibition of Escherichia coli.


Production of a merodiploid strain from a double male strain of E. coli K12.


Transient induction of integration functions in lambda lysogens.


Mutations conferring quantitative and qualitative increases in beta-galactosidase activity in Escherichia coli.


The role of chemotherapy in the treatment of primary ovarian malignancy.


Electron microscopy of excitatory and inhibitory synapses: a brief review.


Structure and function of acetylcholinesterase.


The nature of the acetylcholine pools in brain tissue.


Homo- and heterocellular junctions in cell cultures: an electrophysiological and morphological study.


Soft-tissue calcification in uremia.

Soft-tissue calcification in uremia.


Ferrokinetic studies and erythropoiesis in malaria.


Glutaminase activity of L-asparagine amidohydrolase.


L.H.S. Clark, M.Sc., Ph.D., F. Inst. P.

L.H.S. Clark, M.Sc., Ph.D., F. Inst. P.


Brain surgery for sexual disorders.

Brain surgery for sexual disorders.