Predictive value of liver biochemistry in acute hepatic ischemia.


A modified single layer suture for use in the gastrointestinal tract.


Chocked-clip aneurysmorrhaphy.

Chocked-clip aneurysmorrhaphy.


Replantation of extremities.

Replantation of extremities.


Projections of the interstitial nerve cells surrounding the globus pallidus: a study of retrograde changes following cortical ablations in rabbits.


Dendrodendritic synapses of cells that have axons: the fine structure of the Golgi type II cell in the medial geniculate body of the cat.


Critical study of the specificity of the skin-window test in allergy.


The vacuolisation of eosinophils and its significance in the skin-window test.


Cellular evolution in the skin-window test with a specific antigen.


Metabolism of long-chain fatty acids in the rumen.


Hypertension.

Hypertension.


Perception.

Perception.


Basic drives.

Basic drives.


Behavioral genetics.

Behavioral genetics.


Classification of the behavior disorders.


Student development and counseling.

Student development and counseling.


Behavior therapy.

Behavior therapy.


Effects of aspartic acid, orotic acid, and glucose on serum bilirubin concentrations in infants born before term.


Urinary ascites complicating spina bifida.


John Bamber Hickam.

John Bamber Hickam.


John Bamber Hickam. Physician, educator, investigator.


Single-breath gas distribution in chronic obstructive lung disease. Isoproterenol effect on neon dilution volume and diffusing capacity.


Cheyne-Stokes respiration. A review of clinical manifestations and critique of physiological mechanisms.


Myocardial blood flow. Measurement by a coincidence counting system and single bolus of 84Rb.


Rheumatoid arthritis: reflections on etiology and pathogenesis.


Are taste and drug sensitivity subject to periodic fluctuation?


Messenger and template activities of chemically modified polynucleotides.


An experimental comparison in dogs of expired air and oxygen ventilation during external cardiac massage.


A comparison of pentazocine and pethidine in patients with pain following cholecystectomy.


Premedication in children. A trial of intramuscular droperidol, droperidol-phenoperidine, papaveretum-hyoscine and normal saline.


Further experiences with bezitramide. Its analgesic action and side effects in patients operated upon for lumbar disc protrusion.


Air embolism during neurosurgery in the sitting position. Two case reports.


Effects of radiation pasteurization on Salmonella. II. Influence of repeated radiation-growth cycles on virulence and resistance to radiation and antibiotics.


Potassium binding, growth, and survival of an extremely halophilic bacterium.


Effect of 5-bromouracil on the growth of a thymine auxotroph of Escherichia coli K-12.


End results in reticulum cell sarcoma of bone treated by bacterial toxin therapy alone or combined with surgery and-or radiotherapy (47 cases) or with concurrent infection (5 cases).


Comparative biological activity of nogalamycin and its analogs.


The action of sennosides and related compounds on human colon and rectum. The direct action of intraluminal senna and related compounds on the human colon and rectum has been investigated. Motility was recorded by balloon kymography with recording units inserted into well established transverse colostomies or into the rectum. The motility of the colon was not changed by intraluminal senna glycosides but the introduction of senna previously incubated with faeces or Esch. coli stimulated the colon to peristalt. The peristalsis was similar to that stimulated by rheinanthrone, an oxanthrone produced by chemical hydrolysis and reduction of senna. Both activated senna and rheinanthrone appeared to act in the colon by contact stimulation. No peristaltic response was stimulated in the rectum, either with activated senna or with rheinanthrone.


Lactulose.

Lactulose.


A trial of azathiorrine in primary biliary cirrhosis.


The application of numerical taxonomy to the separation of cllonic inflammatory disease.


Food service.

Food service.


Electrolyte absorption from the colon.

Electrolyte absorption from the colon.


Existential psychotherapy.

Existential psychotherapy.


Dr. Herman Nunberg (1884-1970).

Dr. Herman Nunberg (1884-1970).


Emphysematous cystitis and other radiographic manifestations of diabetes mellitus in dogs and cats.


Uptake of beta-galactosides by Escherichia coli at elevated atmospheric pressure. Uptake of beta-galactosides by Escherichia coli was increased by pressurization to 68 atm. The increase appeared to be due to an effect on the beta-galactoside permeases.


Regulation of thymidine metabolism in Escherichia coli K-12: evidence that at least two operons control the degradation of thymidine. In Escherichia coli K-12, the rise in activity of thymidine phosphorylase, phosphodeoxyribomutase, and deoxyribose-5-phosphate aldolase caused by exogenous thymidine is dependent on the synthesis of new enzyme protein. Phosphodeoxyribomutase is induced by the purine ribonucleosides adenosine and guanosine, whereas the other two enzymes are not. The mutase activity induced by thymidine and by the purine ribonucleosides has been shown to be the same enzyme by four different criteria. This independent induction of phosphodeoxyribomutase suggests that the gene for this enzyme is in an operon different from the one that may contain the genes for thymidine phosphorylase and deoxyribose-5-phosphate aldolase.


Repair of irradiation transforming deoxyribonucleic acid in wild type and a radiation-sensitive mutant of Micrococcus radiodurans. The survival of biological activity in irradiated transforming deoxyribonucleic acid (DNA) has been assayed in the wild type and a radiation-sensitive mutant of Micrococcus radiodurans. The frequency of transformation with unirradiated DNA was lower in the mutant to about the same extent as the mutant's increased sensitivity to radiation. However, in both the wild type and the mutant, the irradiated DNA that was incorporated into the bacterial genome was repaired to the same extent as determined by the loss of transforming activity with increasing radiation dose. This applied to DNA irradiated either with ionizing or ultraviolet (UV) radiation. The rate of inactivation of biological activity after UV radiation was the same in any of the DNA preparations tested. For ionizing radiation, the rate of inactivation varied up to 40-fold, depending on the DNA preparation used, but for any one preparation was the same whether assayed in the wild type or the radiation-sensitive mutant. When recipient bacteria were irradiated with ionizing or UV radiation immediately before transformation, the frequency of transformation with unirradiated DNA fell, rapidly and exponentially in the case of the sensitive mutant but in a more complicated fashion in the wild type. The repair of DNA irradiated with ionizing radiation was approximately the same whether assayed in unirradiated or irradiated hosts. Thus, irradiation of the host reduced the integration of DNA but not its repair.


An additional step in the transport of iron defined by the tonB locus of Escherichia coli.


Proteolytic release of a histidinol dehydrogenase fragment from the double enzyme histidinol dehydrogenase-imidazolylacetol-phosphate: L-glutamate aminotransferase.


Cotransduction of strA and ribosomal protein cistrons in Escherichia coli-Salmonella typhimurium hybrids. Genetic mapping of ribosomal protein cistrons of Salmonella typhimurium and Escherichia coli was performed by phage P1 mediated, generalized transduction. From an E. coli hybrid strain which carried a S. typhimuirum F' factor, an E. coli strain was constructed which had integrated S. typhimurium genetic material including the region of the strA locus. Salmonella genetic material from this hybrid was transduced into E. coli recipients. The ribosomal protein electrophoretic patterns of these hybrid transductants were correlated with the presence of markers contributed by each parent. The results of these studies indicate that cistrons for at least three characteristic S. typhimurium and two E. coli 30S ribosomal proteins are closely linked to the strA locus on the genetic maps of both organisms. At least one cistron coding for a 50S ribosomal protein is also closely linked to this locus on both maps. These findings support the concept that cistrons coding for the ribosomal proteins are clustered in one area of the genome. Mutations to spectinomycin and streptomycin resistance are closely linked in S. typhimurium and are located at strA.


Legal liability for unauthorized autopsies and related procedures.


Clarke E. Chamberlain. April 23, 1895--December 12, 1970.


Protection of kidneys by pepsin degraded immunoglobulin.


The kidney and the calcium paradox.

The kidney and the calcium paradox.


Kidney preservation by initial perfusion and surface cooling.


Body weight changes in overweight patients following an appetite suppressant in a controlled environment.


Acute icclusion of the portal vein in the calf.


Leiomyosarcoma in a renal allograft recipient treated with immunosuppressive drugs.


Origin of margarine.

Origin of margarine.


Clinical studies on proscillaridin a new squill glycoside.


A controlled comparative study of doxepin and chlordiazepoxide in psychoneurotic anxiety.


Trial management in psychopharmacology. The roles and tasks of an industry physician.


The pharmacologist--clinical investigator dialogue in evaluation of new psychotherapeutic drugs.


Historical vignette. The unheroic representation of heroes.


The clinical diagnosis, classification and histogenetic concepts of the early stages of cutaneous malignant melanomas.


Salt induces changes of turbidity and volume of E. coli.


Sequence of 6S RNA of E. coli.

Sequence of 6S RNA of E. coli.


Profile of a refinery nurse. (Holmes P).


Pesticide and growth regulator residues in pineapple.


Early radiology in Wisconsin.

Early radiology in Wisconsin.


Obituary. Dr. Herbert Conway.

Obituary. Dr. Herbert Conway.


The World Federation of Occupational Therapists.


Management of chest injuries.

Management of chest injuries.


Haematological complications of liver transplantation surgery.


Solution of prosthodontic problems through new materials.


The McFarland by-pass procedure in infantile pseudarthrosis of the tibia.


The effects of gastric operations.

The effects of gastric operations.


A quantitative cytophotometric study of protein-bound sulphydryl groups in human palatal cells under experimental prosthetic conditions.


Acrolein fixation for light microscopy of dental tissues.


A scanning electron microscopic observation of etched human peritubular dentine.


The relative significance of preoperative oral antibiotics, mechanical bowel preparation, and preoperative peritoneal contamination in the avoidance of sepsis after radical surgery for ulcerative colitis and Crohn's disease of the large bowel.


Effect of antroneurolysis on gastric secretion in dogs with total gastric fistula.


The operation of orthotopic renal allografting in the pig and its complications.


Gastric secretion following sympathectomy in cats.


The effects of oxethazaine upon acid and pepsin secretion in the dog.


Gastric ulceration complicating experimental liver transplantation.


The comparison of 24-hour preservation by hypothermic perfusion and cold storage on canine kidneys damaged by warm ischaemia.


Proteinuria, lysozymuria, and renal transplantation.


Sodium excretion following cadaveric kidney transplantation.


Bilateral kidney allografts in unselected pigs with caval and portal venous drainage.


Macrophage migration and lymphocyte transformation studies on peripheral blood-cells from human renal allograft recipients.


Lung transplantation: comparison of lung function after unilateral versus bilateral procedures.


Graft viability in orthotopic porcine cardiac transplantation.


The effect of nandrolone phenylpropionate (Durabolin) on the bone-marrow suppression caused by cyclophosphamide.


Objective measurement of platelet activity in vivo.


The role of trauma in the development of peritonitis and the protection afforded by intravenous dextran solutions.


Preservation of the dog heart for 96 hours at 4 degrees C.


Controlled trial of azathioprine and prednisone in chronic renal disease. Report by Medical Research Council Working Party. Patients with various forms of glomerulonephritis, but excluding those with minimal glomerular changes, were admitted to a controlled trial of a regimen which combined azathioprine in a dosage of 2.5 mg/kg/day with prednisone in a dosage of 20 mg/day (adults) or 0.5 mg/kg/day (children). Of 149 patients included, 32 of them under the age of 15, 72 were randomly allocated to the "treatment" group and 77 to the "control" group. There was no evidence of benefit from the treatment group as a whole; and the mortality was in fact higher in the treated group.


Bacteriological and serological studies in preparturient sows.


Stabilizing effects of D2O on the microtubular components and needle-like form of heliozoan axopods: a pressure-temperature analysis.


Murine myeloproliferative virus in cell culture.


Isolation of mycoplasma from leukemic and nonleukemic patients.


Minute virus of mice. I. Procedures for quantitation and detection.


Epstein-Barr virus antigens, marker chromosome, and interferon production in clones derived from cultured Burkitt tumor cells.


Antibacterial activity of certain Mannich bases against Escherichia coli and Staphylococcus aureus.


What happened to the King?

What happened to the King?


Studies on cellular immunity in leprosy. I. Lysosomal enzymes.


Control of uric acid excretion.

Control of uric acid excretion.


International Leprosy Association.

International Leprosy Association.


Fate of Mycobacterium leprae in macrophages of patients with lepromatous or tuberculoid leprosy.


Pulmonary surfactant.

Pulmonary surfactant.


Studies on gut ultrastructure and digestive physiology in Rhabdias bufonis and R. sphaerocephala (Nematoda:Rhabditida).


A comparison of orciprenaline and salbutamol administered orally in 12 adult asthmatic patients.


Clinical trial of salbutamol on bronchial asthma in children.


Salbutamol in status asthmaticus and severe chronic obstructive bronchitis.


Ventilatory results and side-effects of salbutamol given by different routes in coalminers with reversible broncho-obstruction.


Double-blind comparison of isoprenaline, orciprenaline and salbutamol aerosols in patients with asthma.


A comparison of the respiratory effects of SCH-13949W and isoproterenol aerosols in the treatment of patients with expiratory airflow disorders. Preliminary analysis in a long-term trial in ambulatory patients.


A multi-clinic double-blind trial of salbutamol in bronchial asthma.


The treatment of asthma with salbutamol aerosol.


The bronchodilator effects of orciprenaline and salbutamol. A double-blind study.


Comparison of the effects of salbutamol and orciprenaline in asthma.


Slow release salbutamol and Tedral in the treatment of reversible airways obstruction.


Presensitization and kidney transplant failures.


Computers in radiology at Massachusetts General Hospital.


The radiological investigation of hydrocephalus.


On evaluating upper gastrointestinal symptoms by provocative study.


Dedication of "Radiologic Clinics of North America" to Laurence L. Robbins.


Anticholinergic-aided study of the gastrointestinal tract.


Nephrotomography. Performance and interpretation.


Radiologic examination of the gastrointestinal tract in infants and children under 4 years of age.


Radiographic measurement of thoracic gas volume.


Lung biopsy. Bronchial brushing and percutaneous puncture.


Fluoroscopic technique of double contrast arthrography of the knee.


The laboratory diagnosis of lumpy skin disease.


Myoepithelial cells of the sweat glands of domestic animals.


A new technique for measurement of cardiac output by thermodilution in man.


Ethacrynic acid and furosemide. Diuretic and hemodynamic effects and clinical uses.


Hypnosis and smoking: a review of the literature.


Macartney on inflammation. II.

Macartney on inflammation. II.


2'-0-methylcytidine 5'-diphosphate as substrate for polynucleotide phosphorylase from Escherichia coli.


The enhancing effect of ionic strength and MgC12 on the rate of the non-enzymic hydrolysis of N-blocked aminoacyl-tRNA's.


Nucleic acid and ribosome synthesis by Escherichia coli incubated in 5',5',5'-trifluoroleucine.


On the aminoacyl-tRNA binding site of the 30-S ribosomal subunit and its relation to the chain initiation site of the ribosome.


Protein synthesis at high temperatures: aminoacylation of tRNA.


Kinetic preference for initiation of protein synthesis at AUG codons.


The effect of bacteriophage T4 infection on an ATP-dependent deoxyribonuclease in Escherichia coli.


An alteration in ribosome function caused by equimolar binding of erythromycin.


What is hypertension? Recent studies on neurogenic hypertension.


Hypertension and arterial disease.

Hypertension and arterial disease. There is abundant evidence that the height of an individual's systemic arterial pressure is related, in a graded way, to his chances of developing ischaemic heart disease or cerebrovascular disease. The way in which the link operates in respect of coronary disease is not yet known, and further evidence is required before we can accept that the hypertension is necessarily a direct causal factor rather than a marker of some feature in the individual's make-up which is itself linked to the onset of arterial thrombosis (the ;series' or ;parallel' wiring dilemma). In respect of haemorrhagic stroke not only have we a link but we also have a mechanism of action plus evidence that pressure reduction can modify the risk. We have a ;series' wired circuit, and the problem now facing us is to acquire the wisdom which will allow us to decide when to seek out hypertension and what we should do when we find it.


Acute myocardial infarction--progress in primary prevention.


Automation in cardiology.

Automation in cardiology. The basic principles of analogue and digital computers are reviewed and their application is described for the beat-to-beat analysis of the electrocardiogram using the AZTEC data reduction programme, for on-line haemodynamic analyses in the cardiac catheterization laboratory, coronary care unit, or postoperative ward, and for the clinical history and physical examination of the patient. The role of the computer in the management of cardiovascular disease is defined and its superiority in the collection and manipulation of complex data is emphasized. Its use in sparing staff the tedium of routine analyses of data will amply repay the initial cost of its installation.


Regulation of the pulmonary circulation. Factors regulating pressure and flow in the lungs are reviewed with particular emphasis on their role in regulating blood flow velocity and distribution within the lung capillaries. The behaviour of the pulmonary arterial, system, alveolar capillaries, and pulmonary venous system are considered individually. The effect of heart disease on lung capillary blood flow is examined.


Prevention and treatment of ventricular dysrhythmias.


Regulation of the peripheral circulation.


Early anatomical knowledge of cardiac malformation.


Surgical treatment of complex forms of transposition. In spite of the new knowledge developed in recent years surgical treatment for patients with complex forms of transposition of the great arteries remains suboptimal. This is because of the morbidity and mortality that have followed many of the corrective operations, and these are related chiefly to the basic malformation and its secondary effects. This review summarizes information about surgical treatment of such patients and comments upon some of the implications of this experience.


Regulation of coronary blood flow.

Regulation of coronary blood flow. Coronary blood flow is dependent upon arterial pressure, diastolic time, and small vessel resistance. The system is regulated to achieve a low flow high oxygen extraction and low myocardial Po(2). This setting is sensitive to change in oxygen needs. Regulation of blood flow occurs primarily through local intrinsic regulation, most likely through production of vasodilating metabolites in response to minimal degrees of ischaemia. Local regulation appears to dominate over remote regulation in most circumstances. Blood flow distribution to the myocardium is depth dependent as well as regional in variation. Both types of distribution of blood flow are profoundly disturbed in the presence of obstructive coronary atherosclerosis. This results in either concentric myocardial shells or patchy transmural zones of selective ischaemia with clear-cut but local abnormalities in metabolism and performance.


3 and 12 lead electrocardiogram interpretation by computer. A comparison on 1093 patients.


Involvement of veins in Behcet's syndrome.


Ectodermal dysplasias in cattle. Analogues in man.


Acquired hypomelanosis.

Acquired hypomelanosis.


Ventilation during bronchoscopy.

Ventilation during bronchoscopy.


Hyperkalaemia following succinylcholine administration in neurological disorders: a review.


Rheumatism in industry: a review.

Rheumatism in industry: a review.


The suppression of the circulatory action of some neutral lipide soluble fraction (LNF) by the growth hormone releasing hormone (GRH).


Aging under glass.

Aging under glass.


Organismal differentiation, ageing and rejuvenation.


Functional and cellular aspects of organ ageing.


The regulation of protein synthesis in the ageing cell.


Nervous and hormonal mechanisms in the aging process.


The part-time medical officer of health.


Fluorescent antibody studies in three patients dying from honey bee sting.


Infectious resistance to carbenicillin in Proteus rettgeri: occurrence of two different penicillinases in the same strain.


Does indocyanine green obey Beer's law?

Does indocyanine green obey Beer's law?


Venous admixture and lung water in healthy subjects over 50 years of age.


Fifty years' observation of gastric surgery.


Alveolar gas exchanges and cardiovascular functions during breath holding with air.


Limitations of thermal dilution curves for cardiac output determinations.


Rate of development of pulmonary O2 toxicity in man during O2 breathing at 2.0 Ata.


Two mutations affecting utilization of C4-dicarboxylic acids by Escherichia coli.


Tracheal stenosis.

Tracheal stenosis.


My experience at Pearl Harbor at the time of the blitz, December 7, 1941.


The practice of medicine in Hawaii in June, 1916.


Preliminary report on analgesic suppositories.


Experience with cardiac transplantation in fourteen patients.


Experience at Cape Town with human to human heart transplantation.


Results of cardiac transplantation.

Results of cardiac transplantation.


Clinical experience in cardiac transplantation.


Three cases of allogenic heart grafts in humans.


Malaria--a problem for Hawaii?

Malaria--a problem for Hawaii?


Experience of the Montreal Heart Institute.


Experience on three cases of human heart transplantation.


Immunologic considerations for future cardiac transplantation in man.


Heart transplant experience.

Heart transplant experience.


Transplantation of the human heart.

Transplantation of the human heart.


Indications for cardiac transplantation.


Heart transplantation: University of Toronto.


Brain death as seen by the neurosurgeon.


The donor heart: brain death and pathological changes in the heart.


Anesthetic management in nine heart transplantations.


Operative technique for cardiac transplantation.


Myocardial hypothermia for cardiac transplantation.


Storage of the isolated heart.

Storage of the isolated heart.


Evaluation of the condition of the isolated heart during preservation.


Perfusion of the graft during heart transplantation.


About perfusional method of isolated heart preservation before its transplantation.


Report of histocompatibility in 70 heart transplants.


Red cell antigen compatibility in heart transplantation.


Pre-existing antibodies: relevance to heart transplantation.


Intravenous use of high dosage of ALG.

Intravenous use of high dosage of ALG.


Diagnostic criteria of rejection in human heart transplantation.


Electrocardiographic and vectocardiographic signs of rejection.


Complications of prolonged cortisone therapy.


Toxicity of azathioprine.

Toxicity of azathioprine.


Untranslated T7 phage mRNA is stabilized in suA host.


Hepatoportal circulation and its clinical implications.


Human renal allotransplants. Value of physical examination, biochemical, hematologic, and immunochemical testing.


Origins of electrotherapy. I.

Origins of electrotherapy. I.


Measurement of plasma triglyceride turnover in the study of hyperglyceridemia.


Role of duodenum in inhibition of gastric acid secretion. A brohee lecture given at the 4th World Congress of Gastroenterology in Copenhagen 1970.


Effect of oxyphencyclimine hydrochloride on basal gastric secretion of acid and pepsin in man.


Effect of insulin on acid and pepsin secretion in vagotomized and non-vagotomized patients already stimulated by pentagastrin.


Preface to a grammar of biology. A hundred years of nucleic acid research.


L-glutaminase: suppression of lymphocyte blastogenic responses in vitro. Continuous exposure to 0.1 international unit or more of Escherichia coli L-glutaminase inhibited responses of human lymphocytes to phytohemagglutinin, streptolysin O, and allogeneic leukocytes. Inhibition was completety reversed by removing the enzyme from the culture or adding L-glutamine but not L-asparagine. Cytoxicity did not occur. L-Glutaminase should be immunosuppressive in vivo.


DNA polymerase required for rapid repair of x-ray--induced DNA strand breaks in vivo. A much higher yield of DNA single-strand breaks was obtained in the DNA polymerase-deficient mutant Escherichia coli K-12 pol A1 after a given dose of x-rays than had been found before in Escherichia coli. The increased yield of single-strand breaks was due to the absence of a rapid repair system, which had not been described in Escherichia coli K-12. This absence probably accounts for the x-ray sensitivity of the pol A1 mutant. The rapid repair system can be reversibly inhibited in pol+ cells.


Cross-linked transfer RNA functions in all steps of the translation process. A specific photochemical reaction between 4-thiouridine and cytosine cross-links two arms of transfer RNA. This cross-link, introduced into phenylalanine transfer RNA and arginine transfer RNA, limits the conformational freedom of the molecule. Both modified transfer RNA's are capable of functioning in all steps of protein synthesis with this restraint on allowable conformations.


Pulmonary phycomycosis associated with lymphocyte depletion.


Renal transplantation: criteria for selection of donors.


Ultrastructural observations of rat pituitary gonadotrophs following injection of purified porcine LH-RH.


Pituitary GH and hypothalamic GHRF after median eminence implantation of ovine or human GH.


Turnover changes in the tubero-infundibular dopamine neurons during the ovarian cycle of the rat.


Plasma growth hormone responses to intrapituitary injections of growth hormone releasing factor (GRF) in the rat.


A glance at the history of aural surgery.


Clinical evaluation of new contact lens solution.


Acrodermatitis enteropathica successfully treated with diiodohydroxyquin and cow's milk.


A simplified technique for detection of significant bacteriuria.


Immunofluorescent staining of rat gastric parietal cells by human antibody unrelated to pernicious anaemia. Immunofluorescence tests on 94 human sera reacting with rat gastric parietal cells revealed that 41 (44%) of the sera contained antibody to a rat parietal cell antigen that was distinct from the pernicious anaemia autoantigen. Ten of the sera contained antibodies to both parietal cell antigens. The remaining 53 (56%) sera contained only parietal cell antibodies of the pernicious anaemia type. We recommend that mouse gastric mucosa, which does not react with the heterologous rat parietal cell antibody, replace rat gastric mucosa for immunofluorescence diagnostic tests.


Coagulation studies in haemophilic patients taking oral contraceptives. Coagulation studies were performed on haemophilic patients during a controlled trial of oral contraceptive therapy. Treatment with a combined oestrogen-progestogen preparation was associated with a significant rise in the factor VII-X complex. This change was not observed during treatment with progestogen alone. The highest levels of factor IX and fibrinogen and the lowest levels of factor X were observed during the first period of the trial but these effects were independent of the type of treatment. The results suggest that the effects of oral contraceptives on blood coagulation in haemophilic males are similar to those observed in normal females and that the changes are due to the oestrogenic component.


Lessons learned in twenty years experience in treating the chronic lung patient at home with mechanical equipment.


A recessive UGA suppressor.

A recessive UGA suppressor.


On the process of cellular division in Escherichia coli. IV. Altered protein composition and turnover of the membranes of thermosensitive mutants defective in chromosomal replication.


Proportion of HeLa cell genome complementary to transfer RNA and 5 s RNA.


Malaria in drug addicts.

Malaria in drug addicts.


Leprosy vaccines.

Leprosy vaccines.


Von Willebrand's disease.

Von Willebrand's disease.


The concepts of scaling and refractoriness in psychophysical theories of vision.


Adapting coincidence scalers and neural modelling studies of vision.


The theory and indications of hyperbaric oxygen: a review.


Clinical trial of erythromycin stearate suspension.


The immediate autogenous bone graft by the intraoral route.


Control of bacteremia associated with extraction of teeth. II.


Infectious mononucleosis. A review of the literature, including recent findings on etiology.


Physiologic approach to diagnosis of mediastinal disease.


A controlled study of mesoridazine: an effective treatment for schizophrenia.


Simultaneous occurrence of renal allograft rejection in one patient and psychosis in another.


Free peritoneal gas without perforated viscus in patients with bacterial peritonitis.


Spontaneous splenic rupture in lymphoma: report of 3 cases.


Some nineteenth-century pioneers of haematology.


The application of chemical criteria to biological classification in the eighteenth century.


William Cullen: his calibre as a teacher, and an unpublished introduction to his A Treatise of the Materia Medica, London, 1773.


The importance attributed to perceived work competence and scholastic achievement by institutionalized retarded persons.


A historical view of the private residential facility in the training and study of the mentally retarded in the United States.


Imurel therapy in "autoimmune" disease states.


Pathophysiology of gram-negative shock.

Pathophysiology of gram-negative shock.


The social role performance of depressed women: comparisons with a normal group.


China and America: the burden of the past.


Long-term follow-up study of patients with congenital cystic dilatation of the common bile duct.


Metabolism of single carbon compounds.

Metabolism of single carbon compounds.


Virus-induced chromosome abnormalities.

Virus-induced chromosome abnormalities.


Circular dichroism of transfer ribonucleic acid.


Microbial growth rates in nature.

Microbial growth rates in nature.


Immunofluorescent studies of the skin in cryoglobulinaemic vasculitis.


The "pemphigus" antibody and immunopathies affecting the thymus.


Role of lipids in the biosynthesis of the bacterial cell envelope.


The mycosis fungoides cell: the skin window in mycosis fungoides.


Metabolic disorders and hair growth.

Metabolic disorders and hair growth.


Intraspinal tumours in children (excluding dysraphism).


Hazards of mercury. Special report to the secretary's pesticide aedvisory committee, Department of Health, Education, and Welfare, November 1970.


DNA base composition and Adansonian analysis of mycobacteria.


On the cross-reactivity between antigens of Escherichia coli and of gastric parietal cells in man.


Bacterial metabolism and toxicology.

Bacterial metabolism and toxicology.


History of geriatrics.

History of geriatrics.


Double-blind clinical trial of the analgesic effects of phenazocine hydrobromide (Narphen) compared with morphine sulphate in patients with acute abdominal pain. No significant difference could be detected either by clinical impression or statistical analysis in the relief of pain afforded by 2.5 mg phenazocine hydrobromide (Narphen) and 10 mg morphine sulphate when given by intramuscular injection to patients with acute abdominal pain. Phenazocine does not cause spasm of the sphincter of Oddi and so is recommended for treating biliary or pancreatic pain.


Location and measurement of enzymes in single cells by isotopic methods. II. Application to electron microscopy.


Neuronal and glial perikarya preparations: an appraisal of present methods.


Survival and macromolecular synthesis during incubation of Escherichia coli in limiting thymine. Survival and the synthesis of deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and protein were measured during incubation of a thymine auxotroph of Escherichia coli in a series of media containing thymine concentrations below the optimal level of 2 mug/ml. The rate of increase in viable count gradually diminishes to no net growth with 0.2 mug/ml. With lower concentrations of thymine, the rate of cell death gradually increases, resulting in a typical thymineless death curve with 0.02 mug/ml. Both the rate of cell growth and the rate of cell inactivation vary linearly with the thymine concentration. Thirty minutes of incubation in media containing limiting concentrations of thymine before a shift to complete thymine starvation results in a progressive decrease in the length of the lag period preceding thymineless death. These data suggest that only one type of cellular damage occurs during the various degrees of thymine limitation. Prolonged preincubation in media containing 0.1 to 0.2 mug/ml of thymine results in an immunity to thymineless death. This immunity differs from that observed with amino acid-starved cells in its kinetics; ultraviolet irradiation of preincubated cells indicates that the cells are inactivated at the same rate as log-phase cells. These results suggest that the immunity is not associated with chromosome alignment. Thymine concentrations between 2 mug/ml and 0.2 mug/ml permit essentially the same amount of protein and RNA synthesis. The total amount of synthesis then decreases linearly to 40 to 50% of the control level with further reduction in the amount of thymine present. Protein and RNA synthesis are first affected at the same thymine concentration at which lethality is first detectable, and this correlation suggests that the synthesis of these macromolecules is involved in the mechanism of thymineless death. DNA synthesis, on the other hand, is directly dependent on the thymine concentration for levels of 0.5 mug/ml or less. There are no critical changes in DNA synthesis associated with lethality, and DNA synthesis is still occurring under conditions of thymine limitation which result in immunity. These observations suggest that DNA synthesis is not directly involved in thymineless death.


Cellular aspects of primary antibody response in vitro.


Failure to detect homology between the DNA of the Shope fibroma virus and the DNA of the sensitive cell.


One-stage replantation of both lungs in the dog.


Rodent model systems in periodontal disease research.


Studies on the infection of Mycoplasma pneumoniae. I. Application of immunofluorescent antibody method to serodiagnosis of primary atypical pneumonia.


Some of the periodontal ramifications of replantation of avulsed teeth: a review.


Order of the ilv genes of Salmonella montevideo.


Ribosomal proteins. XV. Amino acid compositions of isolated ribosomal proteins from 30S and 50S subunits of Escherichia coli.


The renal circulation.

The renal circulation.


Diagnosis and treatment of syphilis.

Diagnosis and treatment of syphilis.


A century of veterinary pathology at the A.F.I.P., 1870-1970. Dr. Woodward on bovine pleuropneumonia.


Trends in diagnostic radiology.

Trends in diagnostic radiology.


The personality of Guibert of Nogent.

The personality of Guibert of Nogent.


Milestones in oesophageal surgery.

Milestones in oesophageal surgery.


Autotransplantation of the lung: experimental studies on the cape baboon.


Social class and self-derogation: a conditional relationship.


The role of iron in microbial metabolism.


Preoperative preparation of the colon.

Preoperative preparation of the colon.


Physician-author remembers his father, the man who created the dome.


Tryptophan oxygenase and tryptophan metabolism in endotoxin-poisoned and allopurinol-treated mice.


The utilization of selenomethionine by Escherichia coli.


Functional inactivation and appearance of breaks in RNA chains caused by gamma irradiation of Escherichia coli ribosomes. 70S ribosomes and 30S ribosomal subunits from Escherichia coli MRE 600 were exposed to gamma irradiation at -80szC. Exponential decline of activity with dose was observed when the ability of ribosomes to support the synthesis of polyphenylalanine was assayed. Irradiated ribosomes showed also an increased thermal lability. D(37) values of 2.2 MR and 4.8 MR, corresponding to radiation-sensitive molecular weights of 3.1 x 10(5) and 1.4 x 10(5), were determined for inactivation of 70S ribosomes and 30S subunits, respectively. Zone sedimentation analysis of RNA isolated from irradiated bacteria or 30S ribosomal subunits showed that at average, one chain scission occurs per four hits into ribosomal RNA. From these results it was concluded that the integrity of only a part of ribosomal proteins (the sum of their molecular weights not exceeding 1.4 x 10(5)) could be essential for the function of the 30S subunit in the polymerization of phenylalanine. This amount is smaller if the breaks in the RNA chain inactivate the ribosome.


Exchangeable sodium and blood volume in normotensive and hypertensive humans on high and low sodium intake.


Effects of isoproterenol on the pulmonary circulation in patients with chronic obstructive lung disease.


In vitro evaluation of cell-mediated immunity in mice: experiments with soluble and cellular antigens in a spleen-thymus cell leucocyte migration test (LMT).


In vitro reactivity of human lymphocytes in chronic uraemia: analysis and interpretation.


Characterization of IgG complexes in eluates from rheumatoid tissue.


Antinuclear factors and chronic articular inflammation.


Denture-base problems. How to locate and prevent them.


Immunological responses of 'nude' mice.

Immunological responses of 'nude' mice.


Peer review.

Peer review.


Citation for the William John Gies Award to B. Holly Braodbent, Sr.


Citation for the William John Gies Award to Herbert Kurtz Cooper.


Citation for the William John Gies Award to Philip Jay.


Citation for the William John Gies Award to George M. Hollenback.


Citation for the Award of Merit to Henry M. Thornton.


The Treasurer of the College.

The Treasurer of the College.


Etiology of diabetes.

Etiology of diabetes.


A study of Escherichia coli isolated from chronic urinary infection.


Studies in experimental tetanus infection.


A comparison of the effects of ampicillin on Escherichia coli and proteus mirabilis.


Emergence of Shigella sonnei resistant to kanamycin and to nalidixic acid, without exposure to these drugs.


The dienes (mutual inhibition) test in the investigation of proteus infections.


Serological studies on group-B colicines and organisms producing them.


Antigens of the type-1 fimbriae of salmonellae and other enterobacteria.


Parallelisms of medical greatness.

Parallelisms of medical greatness.


Medicine in the American Revolution. I.

Medicine in the American Revolution. I.


The history of anesthesia: the development of anesthesiology in Oklahoma.


Medicine in the American Revolution. II.


The Old-timers' Luncheon of 1956.

The Old-timers' Luncheon of 1956.


The clinical course of patients who have survived heart transplantation.


Management of long term survivors after cardiac allografts in humans.


Pathogenesis of heart rejection phenomenon.


Management of acute rejection in heart transplants.


Chemical prevention of myocardial necrosis.


Antilymphocyte serum and procarbazine compared as immunosuppressants in mice, rats and rabbits.


The minimal inhibitory concentrations of sulphadimethozine and sulphadoxine against Mycobacterium leprae.


Spina bifida occulta causing plantar ulceration.


Characterization of a nonsense mutation affecting the acitivity of the dissociation factor of Escherichia coli ribosomes.


The fate of the bacterial chromosome in P22-infected cells of Salmonella typhimurium.


The fate of newly made DNA in Escherichia coli mutants thermosensitive in DNA synthesis.


Patterns and directions of medical research in West Africa.


A nineteenth century formulary.

A nineteenth century formulary.


Radiation exposure on high-altitude passenger flights.


Selective destruction of cell organelles by laser beam (theory and practical application).


Effects of radiation on artificial lipid membranes.


Role of bacterial membranes in radiosensitization.


Effects of ultraviolet and visible light on nerve fibers and changes in optical properties during nervous activity.


Effects of ionizing radiation on lysosomes and other intracellular membranes.


Surgical treatment of diverticulitis.

Surgical treatment of diverticulitis.


Microelectrophoretic studies on the surface chemistry of erythrocytes.


Nephrostomy in renal transplantation.

Nephrostomy in renal transplantation.


Relationship of blood substitutes to pulmonary changes and volemia.


Auditory evoked potentials from cortex and various subcortical centers.


Studies on lymphocytes and macrophages of mice treated with antithymocytic serum.


Two kinds of trumpet.

Two kinds of trumpet.


An oviparous woman.

An oviparous woman.


Local protein accumulation during gene activation. II. Interferometric measurements of the amount of solid material in temperature induced puffs of Drosophila hydei.


Differential recovery of auxotrophs after penicillin enrichment in Escherichia coli. Various auxotrophs are recovered from a penicillin enrichment cycle with differing efficiencies. Reconstruction experiments indicate that, under starvation conditions in the presence of penicillin, most auxotrophs undergo some death, whereas prolineless mutants are virtually immune to penicillin-induced killing.


Isolation and characterization of supercoiled circular deoxyribonucleic acid from beta-hemolytic strains of Escherichia coli. Covalently closed circular deoxyribonucleic acid (DNA) molecules were isolated by cesium chloride centrifugation in the presence of ethidium bromide from a naturally occurring beta-hemolytic Escherichia coli strain (SC52). The open circular forms have contour lengths of 2.25 +/- 0.1 mum, 24.0 +/- 0.3 mum, and 29.5 +/- 0.5 mum. The beta-hemolytic character of E. coli SC52 can be transferred by conjugation to a nonhemolytic recipient strain. Analysis of the supercoiled DNA of the hemolytic recipient demonstrated that the two large supercoiled DNA molecules of E. coli SC52 are transferred during this event, too. A beta-hemolytic laboratory E. coli strain and several of its derivatives have been shown to contain at least one circular DNA molecule, slightly larger in size than those isolated from E. coli SC52 and its conjugant. The possible significance of these DNA molecules for hemolysin production and transfer is discussed.


Sulfate-reducing bacterium with unusual morphology and pigment content. A dissimilatory sulfate-reducing bacterium was isolated which differed in morphology and pigment content from previously described species. The organism was mesophilic, obligately anaerobic, gram-negative, nonsporulating, long, and slender with one polar flagellum. Whole cells fluoresced red at neutral pH when excited with light at 365 nm owing to the presence of a pink pigment. Desulfoviridin was present. Reduced minus oxidized spectra of whole cells showed peaks in the position of a c-type cytochrome characteristic of Desulfovibrio species and peaks at about 629 and 603 nm. CO difference spectra showed the presence of a CO-binding pigment with a peak at 593 nm. Lactate and pyruvate supported growth in the presence of sulfate but not in its absence. Sulfate, sulfite, and thiosulfate served as electron acceptors for growth. Hydrogenase was present. The deoxyribonucleic acid had a buoyant density of 1.722 g/cm(3) and a guanosine plus cystosine molar percentage of total bases calculated by two different methods of 61.2 or 63.2.


Cell wall polysaccharide biosynthesis by membrane fragments from Streptococcus pyogenes and stabilized L-form. The formation and composition of a cell wall rhamnose-containing polysaccharide by membrane fragments from Streptococcus pyogenes and its stabilized L-form were compared. Also, the effect of prior treatment on the ability of coccal whole-cell and membrane fragments to incorporate radioactivity from thymidine diphosphate-(14)C-rhamnose, and the results of subsequent attempts to remove labeled polysaccharide from such membranes are given. L-form membrane fragments were capable of only 10% uptake of (14)C-rhamnose from this nucleotide as compared with streptococcal membranes. However, once bound, both membrane fragments polymerized rhamnose to the same extent. These findings tend to negate the almost complete lack of polymeric rhamnose within the intact L-form as being due to the absence of membrane enzymes necessary for the transfer of rhamnose from a suitable precursor to membrane acceptor sites or enzymes responsible for rhamnose polymerization. Degradation of labeled rhamnose polysaccharide after isolation from coccal membranes by mild acid hydrolysis showed muramic acid and glucosamine to be attached. This same polysaccharide from L-form membrane fragments was devoid of amino sugars. These data suggest the possible involvement of amino sugars in the attachment of cell wall polymeric rhamnose to the streptococcal cytoplasmic membrane. The absence of attached amino sugars to rhamnose polysaccharide from L-form membrane fragments is discussed in terms of this organism's continued inability for new cell wall formation. The isolation, from streptococcal membrane fragments, of a polysaccharide containing rhamnose and amino sugars common to at least two different streptococcal cell wall-type polymers was demonstrated.


Mutation preventing capsular polysaccharide synthesis in Escherichia coli K-12 and its effect on bacteriophage resistance. A mutant strain of Escherichia coli K-12 was found in which spontaneous mutation to phage T7 resistance occurred at a very low frequency. T7 resistance in the parental strain from which this mutant was derived resulted from a mutation to excess capsular polysaccharide synthesis. The mutation preventing T7 resistance, non-9, inhibited capsule formation when transduced into capsulated strains. The non-9 mutation was cotransducible with his, the gene order in this region being non-9 his Su-1.


Influence of temperature of incubation and type of growth medium on pigmentation in Serratia marcescens. Maximal amounts of prodigiosin were synthesized in either minimal or complete medium after incubation of cultures at 27 C for 7 days. Biosynthesis of prodigiosin began earlier and the range of temperature for formation was greater in complete medium. No prodigiosin was formed in either medium when cultures were incubated at 38 C; however, after a shift to 27 C, pigmentation ensued, provided the period of incubation at 38 C was not longer than 36 hr for minimal medium or 48 hr for complete medium. Washed, nonpigmented cells grown in either medium at 38 C for 72 hr could synthesize prodigiosin when suspended in saline at 27 C when casein hydrolysate was added. These suspensions produced less prodigiosin at a slower rate than did cultures growing in casein hydrolysate at 27 C without prior incubation at 38 C. Optimal concentration of casein hydrolysate for pigment formation by suspensions was 0.4%; optimal temperature was 27 C. Anaerobic incubation, shift back to 38 C, killing cells by heating, or chloramphenicol (25 mug/ml) inhibited pigmentation. Suspensions of washed cells forming pigment reached pH 8.0 to 8.3 rapidly and maintained this pH throughout incubation for 7 days. Measurements of viable count and of protein, plus other data, indicated that cellular multiplication did not occur in suspensions of washed cells during pigment formation. By this procedure utilizing a shift down in temperature, biosynthesis of prodigiosin by washed cells could be separated from multiplication of bacteria.


Induction of pigmentation in nonproliferating cells of Serratia marcescens by addition of single amino acids. Addition of casein hydrolysate to suspensions of washed, nonpigmented, nonproliferating Serratia marcescens incubating at 27 C induced biosynthesis of prodigiosin. Four amino acids of casein hydrolysate, dl-aspartic acid, l-glutamic acid, l-proline, and l-alanine caused formation of pigment when added individually. dl-Ornithine also was effective. Optimal concentrations for maximal pigmentation were 5 to 10 mg/ml; at these high concentrations, d-serine also induced biosynthesis of some prodigiosin. dl-Alanine and -ornithine were as effective as the l-iosomers, but l-glutamic acid and l-proline gave better responses than their racemic mixtures. Kinetics of prodigiosin biosynthesis after addition of dl-alanine (20 mg/ml) were similar to those of cells suspended in 0.2% casein hydrolysate. The other amino acids were less effective. Addition of 5 mg of dl-alanine or casein hydrolysate per ml to minimal medium increased by 30% the amount of prodigiosin formed by growing cells after incubation for 7 days at 27 C. Cultures grown for 7 days at 27 C in 0.2% casein hydrolsate formed more prodigiosin than did suspensions of nonproliferating cells containing individual amino acids or casein hydrolysate. However, more pigment was produced by cells suspended in l-alanine (5 mg/ml) or l-proline (10 mg/ml) than when suspended in 0.4% natural or synthetic casein hydrolysate. Filtrates from suspensions of nonproliferating cells forming pigment in l-proline induced more rapid formation of prodigiosin, but filtrates from suspensions in dl-alanine did not. The data supported the hypothesis that pyrrole groups of prodigiosin may be synthesized from 5-carbon amino acids such as proline, ornithine, aspartic, and glutamic acids, but the role of alanine is unknown.


Valine accumulation by alpha-aminobutyric acid-resistant mutants of Serratia marcescens. alpha-Aminobutyric acid, norvaline, and norleucine, which are analogues of branched-chain amino acids, inhibited the growth of Serratia marcescens. The inhibitory effect of these three analogues was counteracted by branched-chain amino acids. A number of mutants resistant to these analogues were isolated. alpha-Aminobutyric acid-resistant (abu-r) mutants markedly accumulated l-valine in the culture medium, but the other analogue-resistant mutants did not. Acetohydroxy acid synthetase, which seems to be rate-limiting for the biosynthesis of l-valine, was derepressed in abu-r mutants. One of the abu-r mutants, no. 140, which accumulated over 8 mg of l-valine per ml, had about a 20-fold increase in the enzyme level. Most of the abu-r mutants had acetohydroxy acid synthetase activity which was sensitive to feedback inhibition by l-valine to the same extent as in the parent strain. However, the enzyme of two of abu-r mutants was less sensitive to l-valine, and one of the two was the best valine accumulator.


Dark repair of ultraviolet-irradiated deoxyribonucleic acid by bacteriophage T4: purification and characterization of a dimer-specific phage-induced endonuclease. The purification and properties of an ultraviolet (UV) repair endonuclease are described. The enzyme is induced by infection of cells of Escherichia coli with phage T4 and is missing from extracts of cells infected with the UV-sensitive and excision-defective mutant T4V(1). The enzyme attacks UV-irradiated deoxyribonucleic acid (DNA) containing either hydroxymethylcytosine or cytosine, but does not affect native DNA. The specific substrate in UV-irradiated DNA appears to be pyrimidine dimer sites. The purified enzyme alone does not excise pyrimidine dimers from UV-irradiated DNA. However, dimer excision does occur in the presence of the purified endonuclease plus crude extract of cells infected with the mutant T4V(1).


Interaction between the first enzyme for histidine biosynthesis and histidyl transfer ribonucleic acid. Previous studies suggested that phosphoribosyltransferase, which catalyzes the first step of the pathway for histidine biosynthesis in Salmonella typhimurium and which is sensitive to inhibition by histidine, plays a role in repression of the histidine operon. Recently, we showed that the enzyme has a high affinity for histidyl transfer ribonucleic acid (His-tRNA), which is known to participate in the repression process. In the present study, we have investigated further the interaction between the enzyme and His-tRNA. We found that His-tRNA binds at a site on phosphoribosyltransferase distinct from the catalytic site and the histidine-sensitive site; that the substrates of the enzyme inhibit the binding of His-tRNA, whereas histidine does not do so; that, once a complex has been formed between phosphoribosyltransferase and His-tRNA, the substrates of the enzyme decrease the stability of the complex, whereas histidine is without effect; and that purified phosphoribosyltransferase which has a defect in its inhibition by histidine (produced by mutation) displays an altered ability to bind His-tRNA, a finding which may be a reflection of the fact that mutants producing such a defective enzyme display an alteration of the repression process.


Impaired transduction of R213 and its recovery by a homologous resident R factor. Transduction by Plkc of drug-resistance markers of the factor R213 was shown to occur at an exceptionally low frequency (at less than 10(-8) of the input phage), and they could not be transduced by P22. When the recipient cells carried a homologous R factor derived from R213, markers were transduced by Plkc at a normal frequency (at about 10(-5) to 10(-6) of the input phage). Derivative R factors, transducible by Plkc at a normal frequency but being transferred by conjugation at a frequency lower than that of the original R213, were obtained. This type of transductant often segregated R(-) cells. In addition, several transductants contained R factors which were transferred normally by conjugation but were transduced by Plkc at as low a frequency as the original R213. This type of transductant was an effective recipient for transduction by Plkc of R213 when apparently "cured" by acridine treatment. No such effective "cured" recipients were obtained from the transductants with derivatives of R213 transducible at a normal frequency. Two possible interpretations are presented: (i) R213 produces a bacteriocin-like substance upon transduction, or (ii) the genome size of R213 is too large for all of its determinants to be transduced.


Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants. Eighty-four transfer-deficient mutants of Flac have been isolated; 27 of these bear amber mutations and 1 mutant is temperature-sensitive. All the mutants transfer between 10(-2) and <10(-5)% as well as wild-type Flac, all are curable by acridine orange treatment, and all are resistant to the female-specific phage phi(II). Some of the mutants are partially sensitive to female-specific phage tau. Sixty-three of the mutants are resistant to the male-specific phages f1, f2, and Qbeta; 15 are resistant only to f2; and 6 are sensitive to all three male-specific phages. Most of the mutants are still poor recipients in conjugation, but four of the mutants resistant to f1, f2, and Qbeta have become good recipients. Those mutants resistant to all three male-specific phages do not seem to make F-pili.


Effect of gene induction on the rate of mutagenesis by ICR-191 in Escherichia coli. ICR-191, an acridine half-mustard known to cause frameshift mutations in bacteria, was used to induce Lac(-) mutations revertible by ICR-191. The reversion rates of several of these mutations were stimulated approximately twofold by the presence of lac inducer. The stimulatory effect of inducer was attributable to gene induction rather than some other effect of inducer, since inducer did not stimulate reversion in a regulator constitutive strain. The stimulatory effect was not observed unless the gene to be reverted was induced during the period of exposure to ICR-191. The presence of a strong polar (nonsense) mutation on the operator side of a frameshift mutation abolished the stimulatory effect of inducer on reversion of the frameshift mutation by ICR-191. (As expected, a nonpolar mutation on the operator side of the frameshift mutation did not affect inducer-stimulated reversion.) It was concluded that some aspect of transcription or translation, or both, in the neighborhood of the ICR-191-induced mutation stimulated reversion by ICR-191. A recA mutation had no effect on reversion by ICR-191 in the presence or absence of inducer. In one mutant, gene induction depressed reversion by ICR-191 about sevenfold. The difference between this exceptional strain and other mutants was not attributable to different genetic backgrounds but seemed to be an inherent difference in the original Lac(-) mutations.


Pleiotropic effect of the rec A gene of Escherichia coli: uncoupling of cell division from deoxyribonucleic acid replication. A defective recA gene, which is involved in recombination, is shown in this article to permit limited cell division, when deoxyribonucleic acid (DNA) synthesis is blocked. Thymidine starvation or nalidixic acid blocked DNA synthesis, and stopped cell division of a rec(+)thy(-) strain of Escherichia coli. However, with the same treatments, a recAthy(-) strain could continue to divide for at least 5 hr, and cell numbers increased 2.5- to 4-fold. After several hours of thymidine starvation, the culture contained very long cells (snakes) and small (normal-sized) cells. The short cells contained very little, if any, DNA. Cells of all ages divided in the absence of thymidine. Specific differences in membrane proteins were observed between thymidine-starved rec(+) and recA cells, as expected from previous experiments in which these proteins were associated with cell division and DNA synthesis. It is proposed that septum formation is controlled negatively by the recA(+) gene.


Morphogenetic aspects of murein structure and biosynthesis. The shape of Escherichia coli is fixed by the form of the sacculus. This sacculus is a macromolecule made up from the polymer murein. In an investigation of the possible factors determining the shape of the sacculus, we attempted to resolve between two fundamental alternatives. (i) Is the shape of the sacculus automatically fixed by its chemical composition? or (ii) does a special morphogenetic system exist which determines the shape of the sacculus? An analysis of sacculi from cells grown in poor and rich media and harvested at different stages of growth was made. Significant variations in the composition of murein were found, whereas the general shape of the cells remained unchanged. This finding stands opposed to the assumption of a strict correlation between chemistry and shape of the sacculus. The second alternative was investigated by attempting to change artificially the shape of the sacculus by modifying the form of the hypothetical morphogenetic system. Rod-shaped cells were converted into spherical spheroplasts which were subsequently allowed to reform a new spherical sacculus. In chemical composition this spherical sacculus was found to be indistinguishable from the rod-shaped sacculus. This finding is taken as evidence for the existence of a distinct morphogenetic apparatus in the cell wall whose form is reflected by the shape of the sacculus.


Quantitative aspects of deoxyribonucleic acid renaturation: base composition, state of chromosome replication, and polynucleotide homologies. The base composition of a deoxyribonucleic acid (DNA) sample affects its intrinsic rate of renaturation. In agreement with the information of Wetmur and Davidson, it was established that high guanosine plus cytosine (GC) DNA renatures faster than expected from analytical measurement of its molecular weight. A calculated correction factor of 1.8% of the observed C(0)t(.5) is required for every mole per cent GC difference from 51% GC. The correction factor is now established in the range of 32 to 65% GC. Renaturation of DNA mixtures prepared from pairs of organisms has been studied. When no similarity existed between the two organisms, the observed C(0)t(.5) of the mixture was the sum of the independently determined C(0)t(.5) values. Lack of additivity was correlated with similarities in polynucleotide sequence of the reassociating DNA molecules. A quantitative relationship was formulated to relate C(0)t(.5) values of renatured DNA mixtures to per cent binding ("homology"). Finally, it was demonstrated that DNA prepared from log-phase cells renatures faster than stationary-phase DNA and also departs from theoretical second-order kinetics.


Membrane association of conjugally transferred deoxyribonucleic acid in Escherichia coli minicells. The conjugally acquired deoxyribonucleic acid (DNA) of small, anucleate cells ("minicells") of a mutant strain of Escherichia coli K-12 was found to be predominantly associated with the bacterial membrane. Evidence from X-irradiation studies in vivo shows that there is no decrease in DNA-membrane association under conditions which reduce the DNA to one-sixth its original size and suggests the possibility of multiple DNA-membrane association sites. Preliminary enzymatic studies indicate the involvement of protein, DNA, and lipids in the membrane association of the DNA.


Hyphal wall compositions of marine and terrestrial fungi of the genus Leptosphaeria. Hyphal wall compositions of six Leptosphaeria species were compared to assess whether gross changes have occurred in the hyphal wall chemistry of closely related fungi which have become ecologically restricted to marine or terrestrial habitats. Unfractionated, lipid-extracted hyphal walls of each Leptosphaeria species had qualitatively identical compositions consisting of glucose, mannose, galactose, glucosamine, amino acids, and traces of galactosamine. Quantitative analyses showed that the hyphal wall components varied from species to species. Qualitative compositions of alkali-soluble wall fractions from each species were identical and contained the same sugars found in the unfractionated walls. The alkali-insoluble residues contained glucose, glucosamine, and amino acids. The alkali-soluble fractions were composed predominantly of glucose, galactose, and mannose. The alkali-insoluble fractions contained high concentrations of glucose and glucosamine and relatively low concentrations of amino acids.


Penicillin and cell wall synthesis: a study of Bacillus licheniformis by electron microscopy. The changes in wall structure of two penicillinase-negative strains of Bacillus licheniformis on addition of penicillin were studied. After addition of penicillin to give a concentration of 1 unit/ml, exponentially growing cells of strain 749 c/72 doubled once and then stopped. Strain 749c/72/IIIg was more resistant and continued growing, but synthesis appeared to become uncontrolled over the surface, producing localized wall thickening at the expense of elongation, and leading to distorted cells and growth in twisted and coiled chains, with an accompanying drop in growth rate. The continued growth can be explained by the existence of a less sensitive transpeptidase, but there is no obvious explanation for the uncontrolled synthesis. The effect of penicillin could be reversed by addition of penicillinase in both strains, although there appeared to be a persistent effect of penicillin which also produced distorted cells for a few generations and inhibited cell separation. The changes in wall structure produced by penicillin and penicillinase appeared all over the cell surface, suggesting that wall synthesis occurred all over the cell. Also a separate process for cross-wall synthesis is suggested since this appeared less sensitive than wall synthesis.


Demonstration by freeze-etching of a single cleavage plane in the cell wall of a gram-negative bacterium. In the examination of protoplasts of a gram-negative bacterium classified as a Pseudomonas sp. by freeze-etching, we found a smooth external surface which is not seen if the preparations are not "etched." This external structure is seen as a sleeve surrounding and connecting the cells in unetched preparations, and we present evidence that it is a eutectic formed during the freezing of the specimen. In the system used in this study, the four layers of the cell wall of a gram-negative bacterium can be removed from the cell. The single cell wall cleavage plane is not affected by the removal of the loosely bound outer layer or of the peptidoglycan layer, but it is lost when the outer double track layer and the underlying soluble layer are simultaneously removed. Thus, we conclude that it is one of these two layers which is responsible for the cleavage plane which exposes variable areas of a smooth surface in the cell wall. This cell wall cleavage plane is more likely to deflect the actual cleavage of the frozen cell when cells are relatively old or when they are suspended in sucrose.


Surface structure of Chromatium okenii and Chromatium weissei. The outermost structured layer of both Chromatium weissei and C. okenii is composed of a hexagonal array of hollow cone-shaped subunits approximately 25 nm in length and 13 nm in diameter.


Low-level reading of the UGA triplet in Salmonella typhimurium. Evidence indicating low-level reading of the UGA triplet was obtained for six strains of Salmonella typhimurium. Such low-level UGA "suppression" may be a general phenomenon common to strains of S. typhimurium and, perhaps, to all enteric bacteria.


Cell wall growth in Bacillus licheniformis followed by immunofluorescence with mucopeptide-specific antiserum. Insertion of new wall during the growth of Bacillus licheniformis occurred discretely and probably at the sites of incipient cross wall formation.


Comment on the use of chloramphenicol to study the initiation of deoxyribonucleic acid synthesis. Chloramphenicol cannot be used to distinguish different steps in the initiation of bacterial chromosome replication.


Development of a transplantation team in Indiana. 3. Postoperative medical management of the transplant patient.


Rupture of a mediastinal vein detected by 131-I-MAA scanning.


"Doing dope".

"Doing dope".


Personality and duplicate bridge.

Personality and duplicate bridge.


Suppression of UGA codon by a tryptophan tRNA.


Filamentous bacterial viruses. I. DNA synthesis during the early stages of infection with fd.


Filamentous bacterial viruses. II. Killing of bacteria by abortive infection with fd.


A mutation increasing the amount of a constitutive enzyme in Escherichia coli, glucose 6-phosphate dehydrogenase.


Identification of an ochre-suppressing anticodon.


Divergent orientation of transcription from the biotin locus of Escherichia coli.


Studies of the cerebral circulation: what they have taught us about stroke?


S. Weir Mitchell: nerves, peripheral and otherwise.


Past, present, and future of the Mayo Department of Neurology.


Buffy-coat observations and red-cell antibodies in acquired hemolytic anemia.


Effectiveness of betamethasone in management of severe infections. A double-blind study.


Data on the cause of leukemia.

Data on the cause of leukemia.


How relevant is Kornberg polymerase?

How relevant is Kornberg polymerase?


Host cell reactivation in strains of E. coli lacking DNA polymerase activity in vitro.


Ultraviolet mutagenesis in strains of E. coli deficient in DNA polymerase.


Evaluation of impedance cardiac output in children.


The effectiveness of methylphenidate hydrochloride (ritalin) on learning and behavior in public school educable mentally retarded children.


Studies on the effect of alpha-lipoic acid on disturbances in histidine metabolism.


Drug efficacy study: FDA yields on fixed combinations.


Lead suppression of mouse resistance to Salmonella typhimurium. Mice were treated with subclinical doses of lead nitrate for 30 days. Lead-treated mice showed greater susceptibility to challenge with Salmonella typhimurium than controls which received no lead. This result confirms the hypothesis that treatment with lead reduces the resistance of mice to bacterial infection.


Lymph node cells: their differential capacity to induce tolerance of heart and skin homografts in rats. Rats of the BN strain, inoculated at birth with (Lewis x BN)F(1) hybrid lymph node cells are not tolerant of Lewis skin grafts but do display high degrees of tolerance of Lewis hearts.


Tissue reaction and recovery following experimental tooth movement.


35S incorporation into bacterial RNA.

35S incorporation into bacterial RNA.


Inhibition of thymidylate synthetase by 5-formyl- and 5-hydroxymethyl-2'-deoxyuridylate.


Studies on the enzymatic synthesis of 2,3-dihydroxy-N-benzoyl-L-serine in Escherichia coli.


Four-thiouracil content of chicken liver mitochondrial transfer ribonucleic acid.


Evidence for two types of subunits in succinyl coenzyme A synthetase.


Our first president.

Our first president.


Chondrosarcoma. Immune reactions of a patient to autologous tumor.


Experience with emetine hydrochloride (NSC 33669) as an antitumor agent.


Apparent chemotherapeutic cure of malignant lymphoma.


Testicular morphology and germ cell DNA synthesis in the testis of patients with prostatic carcinoma.


Nuclear DNA content of occult sclerosing and frank papillary carcinoma of the thyroid.


Secondary reconstruction in maxillo-facial trauma.


Quantitative immunofluorescence. Standardization and calibration in microfluorometry.


Research in cardiovascular diseases.

Research in cardiovascular diseases.


Eating patterns before and after dentures.


Blood transfusion: a review of the treatment of the oral surgical patient and report of case.


The reliability of pre-extraction records for complete dentures.


Esthetics--an enigma.

Esthetics--an enigma.


Clinical evaluation of the gagging denture patient.


Modified impression technique for hyperplastic alveolar ridges.


Impression techniques for attachment-retained distal extension removable partial dentures.


Chrome-cobalt alloy pontics for fixed partial dentures.


Prosthetic management of edentulous mandibulectomy patients. 3. Clinical evaluation.


The Federation of Prosthodontic Organizations: The voice of prosthodontics.


Molecular packing in collagen.

Molecular packing in collagen.


Interaction between an amide nitrogen lone pair and a noncontiguous carbonyl carbon and its effect on amide rotation.


Biochemical model for the biological methylation of mercury suggested from methylation studies in vivo with Neurospora crassa.


Recommended procedures for testing genetic hazards from chemicals, based on the induction of dominant lethal mutations in mammals.


Neutron radiography of osseous tumours.

Neutron radiography of osseous tumours.


Induction of cleft palate in rats with intra-amniotic corticoids.


Carcinogenicity of industrial chemicals propylene imine and propane sultone.


Central visual discharge time-locked with spontaneous eye movements in the cat.


Oxytocin antibody and lactation and parturition in rats.


Control of water movement in flounder urinary bladder by prolactin.


Possible hepatic function for crustacean blood cells.


Production of an insect sex attractant by symbiotic bacteria.


Steroids and squalene in Methylococcus capsulatus grown on methane.


Independence of protein synthesis and drug uptake in nerve cell bodies and glial cells isolated by a new technique.


Wounds, Williamsburg, and war. The day "the world turned upside down".


Reconstruction of extensive forehead defects.


Organ transplantation (skin, kidney, heart) and the plastic surgeon.


Personal recollections of the organization and founders of the American Association of Plastic Surgeons.


Some aspects of the character and personality of Jacques Joseph.


A new approach to the correction of cleft lip nasal deformities.


Dislocation of upper lateral cartilages as a cause for nasal deformities.


Effects of triamcinolone on tendon healing and function. A laboratory study.


The terminology of flaps.

The terminology of flaps.


The classic reprint. The palate suture. A newly discovered method to correct congenital speech defects. Dr. Carl Ferdinand von Graefe, Berlin.


An inexpensive aid for flap planning. The map mileage indicator.


Hemopoietic stem cells as a function of erythropoiesis.


Bone marrow, spleen, and thymus regeneration patterns in mice after whole-body irradiation.


Central venous pressure and blood volume determinations in clinical shock.


Regional capillary blood flow and oxygen uptake in severe sepsis.


Medical morals, medical ethics, and Medicare.


The esophagus, the vagi, and fat absorption.


Some metabolites of specifically  tritium labelled lysine-vasopressin: identification by thin-layer chromatography and determination by the liquid scintillation technique.


Application of immunofluorescence to studies on the ecology of Sphaerophorus necrophorus. Sphaerophorus necrophorus (bovine liver abscess isolates) antiserum was fractionated and labeled with fluorescein isothiocyanate. The fluorescent-antibody (FA) conjugate proved to be species-specific and facilitated the detection of S. necrophorus cells in liver abscesses, viscera, and ruminal contents of cattle. Brightly fluorescing S. necrophorus cells were observed in experimentally inoculated soil incubated anaerobically at 37 and 4 C for 8 and 10 months, respectively. When incubated under moist conditions (80% water holding capacity) at 37 C, the test organism survived in both sterile and unsterile soil for as long as 8 weeks. Results strongly support the feasibility of using FA techniques concurrent with cultural methods for rapid detection of S. necrophorus infections.


Further evaluation of the automated fluorescent treponemal antibody test for syphilis. Improvements in the equipment for the automated fluorescent treponemal antibody (AFTA) test for syphilis prompted this comparative study of the AFTA and its manual counterpart, the fluorescent treponemal antibody-absorption (FTA-ABS) test. The AFTA equipment operated satisfactorily, required only minimal monitoring, and afforded a three-to fourfold increase over the number of sera that could be tested manually by one serologist. The AFTA and FTA-ABS tests agreed well with only 2.1% of the sera yielding conflicting results. The AFTA was less precise than the FTA-ABS on sera retested because of original conflicting results and on sera retested within the same run to determine reproducibility. However, these differences were not large, and AFTA test performance was considered to be within the limits acceptable for a diagnostic serological procedure.


Bacteriocin (Marcescin) typing of clinical isolates of Serratia marcescens. A simple, reproducible technique is described for bacteriocin typing of clinical isolates of Serratia marcescens. With 10 marcescin-producer strains, a total of 46 of 50 isolates (92%) of S. marcescens could be typed and categorized into 16 provisional types according to their sensitivity or tolerance to marcescins. The potential significance of this procedure with regard to delineation of outbreaks of nosocomial infection is discussed on the basis of preliminary epidemiological data.


Disc-agar diffusion microbiological assay procedure for determining serum and urine levels of sulfacytine and other sulfonamides. A reasonably precise, reproducible, and sensitive microbiological procedure for directly assaying sulfacytine and other sulfonamides as antibacterially active drugs has been developed by appropriately modifying the standard disc-agar diffusion technique. Blood and urine levels as low as 3 mug/ml may be determined through the use of a strain of Escherichia coli and a chemically defined agar medium devoid of sulfonamide antagonists. Results indicate that this assay method should be a useful adjunct to the Bratton-Marshall colorimetric procedure, by permitting the direct measurement of antibacterially active drug in clinical specimens.


Assay system for bacteriocins.

Assay system for bacteriocins. Bacteriocin activity can be detected and assayed by a modification of the punchhole method.


Detection of Leptospira in soil and water by immunofluorescence staining. Specific immunofluorescence staining was used for the detection of leptospires in soil and water under laboratory and field conditions.


Classification of hyperlipidaemias and hyperlipoproteinaemias. Many studies of atherosclerosis have indicated hyperlipidaemia as a predisposing factor to vascular disease. The relationship holds even for mild degrees of hyperlipidaemia, a fact that underlines the importance of this category of disorders. Both primary and secondary hyperlipidaemias represent such a variety of abnormalities that an internationally acceptable provisional classification is highly desirable in order to facilitate communication between scientists with different backgrounds.The present memorandum presents such a classification; it briefly describes the criteria for diagnosis of the main types of hyperlipidaemia as well as the methods of their determination. Because lipoproteins offer more information than analysis of plasma lipids (most of the plasma lipids being bound to various proteins), the classification is based on lipoprotein analyses by electrophoresis and ultracentrifugation. Simpler methods, however, such as the observation of plasma and measurements of cholesterol and triglycerides, are used to the fullest possible extent in determining the lipoprotein patterns.


Microbiological assay and tissue distribution of 5-diazouracil in mice. A sensitive, precise microbiological assay has been developed for the determination of tissue distribution of 5-diazouracil, a potential antitumor and antimicrobial agent.


Biosynthesis of phage-specific initiation dipeptides. A purified biosynthetic system derived from Escherichia coli.


Acyl phosphatidylglycerol. A new phospholipid from Salmonella typhimurium.


Genetic and metabolic control of enzymes responsible for histidine degradation in Salmonella typhimurium. 4-imidazolone-5-propionate amidohydrolase and N-formimino-L-glutamate formiminohydrolase.


The two operons of the histidine utilization system in Salmonella typhimurium.


Mutants of bacteriophage T4 defective in the induction of T4 endonuclease II.


The general occurrence of 55 S ribosomes in mammalian liver mitochondria.


Antigenic markers on cells leaving calf thymus by way of the efferent lymph and venous blood.


Deposition of immune complexes in the kidneys of mice infected with lactic dehydrogenase virus.


Binding of a polypeptide antigen to ribonucleic acid from macrophage, HeLa and Escherichia coli cells.


Nephritogenic glycoprotein. I. Proliferative glomerulonephritis induced in rats by a single injection of the soluble glycoprotein isolated from homologous glomerular basement membrane.


Antibody plaque responses of mice given Vibrio cholerae antigen as neonates.


Early accumulation of antibody plaque-forming cells in mouse spleens lacking a pre-existing immune background.


Immunosuppressive effects of incomplete Freund's adjuvant on hemolytic antibody-forming cells in spleens of immunized mice.


Antigenic competition at the cellular level: response of rabbit lymph node cells to the trinitrophenyl and p-arsanilic hapTENS.


The number of antibody-producing cells in the parathymic lymph nodes and the spleen following intraperitoneal injection of antigen.


Use of chromium chloride as the coupling agent in a modified plaque assay. Cells producing anti-protein antibody.


Selective inhibition of plaque-forming cells by bursa-specific antisera.


The histomorphologic criteria of beginning stromal invasion in the squamous cell carcinoma of the cervix.


Cytologic diagnosis by computer.

Cytologic diagnosis by computer.


The advantages of a computerized program of population screening for cervical cancer.


The role of nucleoside phosphorylases in the degradation of deoxyribonucleosides by thymine-requiring mutants of E. coli.


Induction of prophage lambda in a mutant of E. coli K12 defective in initiation of DNA replication at high temperature.


Post-blastocyst differentiation in vitro.


X-irradiation sensitivity in Escherichia coli defective in DNA replication.


Protection of irradiated lambda phage by methylated proflavine.


Environmental fluctuations and population size.


Antitrachoma activity of rifamycin B and 8-O-acetylrifamycin S.


Deleterious mutations and neutral substitutions.


Immunological implications of ultrastructural studies of goat X sheep hybrid placentae.


Survival of tumours after immunization against oestrogens.


Triple chromosome pairing in triploid chickens.


Persistence of dose related behaviour in mice.


Identification of a volatile constituent formed by homogenates of Acacia georginae exposed to fluoride.


Visual contour masking.

Visual contour masking.


Red photoreceptor in butterflies.

Red photoreceptor in butterflies.


Flying ability of Archaeopteryx.

Flying ability of Archaeopteryx.


Rules and regulations for test-tube babies.


Monod's new parish.

Monod's new parish.


Protecting predators.

Protecting predators.


No cancer remission for NIH.

No cancer remission for NIH.


Thermal effects.

Thermal effects.


Social values and research in human embryology.


Drug receptors and their function.

Drug receptors and their function.


A study of some factors affecting the in-vitro release of drug from prolonged release granulations. 3. Effects of particle size, enzymatic contents of pepsin and pancreatin, bile and ionic concentration.


The decision to treat myelomeningocele on the first day of life.


Hamilton Bailey.

Hamilton Bailey.


An autoradiographic study of the normal lymphoid cell decay in the mesenteric lymph node of the mouse.


Elimination of intercurrent death among rabbits inoculated with Treponema pallidum.


Three-dimensional reconstruction in histology. A short survey of available methods and description of a new technique.


Biopsies from human renal allografts studied by immunofluorescence.


Mediated transport of glucose between blood and brain in the cat.


Plasma factor VIII synthesis and control as revealed by canine organ transplantation.


Relationship between renal artery perfusion pressure and tubular sodium reabsorption.


Red cell and albumin flow circuits during skeletal muscle reactive hyperemia.


The attributes of genius from Newton to Darwin (John Hunter).


Background details in Reynolds's portrait of John Hunter.


Humoral antibody response in rabbits after intragingival injections of bovine serum albumin and Escherichia coli endotoxin.


The value of a plastic adhesive drape in the prevention of wound infection. A controlled trial.


A technique for the insertion of Holter ventriculoatrial shunt for infantile hydrocephalus.


Complications from Holter ventriculo-atrial shunts.


Evolution of syphilitic chancres with virulent Treponema pallidum in the rabbit.


Attitudes of hospitals in London to venereal disease in the 18th and 19th centuries.


Clinical evaluation of the T. pallidum haemagglutination test.


Incomplete agglutinins against Treponema pallidum.


Test patterns of yaws antibodies in New Zealand.


[Chromatographic methods in the analysis of protein structure. The methods for identification of N-terminal amino acids in peptides and proteins. A].


Trace-element analysis in clinical chemistry.


Interference of human spermatozoa motility by Escherichia coli.


Morphological changes in the spleen of irradiated mice nine days after the transplantation of homologous bone marrow.


Immunofluorescent localization of enteroglucagon cells in the gastrointestinal tract of the dog. Localization of the endocrine polypeptide cells responsible for ;glucagon-like immunoreactivity' in the gastrointestinal tract of the dog has been achieved with an immunofluorescent technique using antibodies raised against porcine pancreatic glucagon. The cells, for which we prefer the term ;enteroglucagon', could only be demonstrated by this technique in tissues fixed in carbodiimide. The enteroglucagon cells possess cytological, cytochemical, and ultrastructural characteristics in common with those of the pancreatic alpha(2) cell and they are equivalent in the stomach to the A cell and in the intestine to the L cell of the Wiesbaden terminology. Their distribution, predominantly in fundus and jejunum, correlates precisely with the distribution of glucagon-like immunoreactivity by radioimmunoassay and bioassay. The storage form of enteroglucagon differs in many respects from that of pancreatic glucagon although there are some close resemblances between the two forms of specific hormone-containing granule. Elucidation of the role of enteroglucagon should be assisted by the ability to demonstrate enteroglucagon cells.


The G cells in pernicious anaemia.

The G cells in pernicious anaemia. An indirect immunofluorescence technique, using the globulin fraction of rabbit antihuman gastrin serum, was applied to formalin-fixed material obtained by suction biopsy from the fundic mucosa of nine cases of pernicious anaemia. Cytochemical tests for endocrine polypeptide cells of the APUD series, in which the G cell is included, were carried out in parallel with immunofluorescence and with ultrastructural observations.G cells were present, in large numbers, in five of the nine cases studied. In the remaining four cases the predominantly intestinalized glands contained only enterochromaffin in cells. Because of their low gastrin content (immunofluorescence), low secretion granule content (cytochemistry), and the associated ultrastructural findings, it is suggested that the G cells of the fundic mucosa are in a state of high synthetic and high secretory activity.


Halothane hepatitis.

Halothane hepatitis.


Fibreendoscopy of the intestines.

Fibreendoscopy of the intestines.


Cyril Barnett: research worker and teacher in anatomy.


Salivary gland inclusion in the anterior mandible. Report of a case with a review of the literature on aberrant salivary gland tissue and neoplasms.


The bacteria-removal efficiency of mechanical lavage and rubber-bulb syringe irrigation in contaminated avulsive wounds.


The Darwinian revolution in the concept of time.


On Hegel--a study in sorcery.

On Hegel--a study in sorcery.


Time and the modern self: Descartes, Rousseau, Beckett.


Concerning the uptake of lipid by the nucleus.


Experimental modification of structures at the periphery of the liver cell nucleus.


Fibrils in marsupial enamel tubules.

Fibrils in marsupial enamel tubules.


The lateral cervical nucleus in the cat. V. A quantitative evaluation on the bouton- and glia-covered surface area of different LCN-neurons.


The pancreatic islets of the teleost Scorpaena scropha. An ultrastructural study with particular regard to fibrillar granules.


In vitro antibacterial activity of trimethoprim and sulphonamides on bacteria causing bovine mastitis.


The binding of the parental DNA from bacteriophages to the cell membrane of Escherichia coli.


Formation of complex Col E1 DNA by replication.


A ribosome-like particle accumulated at low temperature by a cold-sensitive mutant of Salmonella typhimurium.


Reactivation in vitro of inactive ribosomes from stationary phase Escherichia coli.


Studies with hydroxyurea. The biologic and metabolic properties of formamidoxime.


Two comparisons of the analgesic activity of orally administered pentazocine, dihydrocodeine and placebo.


Anti-emetic and propulsive peristaltic properties of metoclopramide.


Iron metabolism in the porphyrias.

Iron metabolism in the porphyrias.


Immunization with irradiated tumour cells and specific lymphocyte cytotoxicity in malignant melanoma. A microculture technique has been adapted to assay the cytotoxic properties of the lymphocytes from patients with malignant melanoma when cultured with their autologous tumour cells. In a series of patients with established melanoma specific autologous cytotoxicity was extremely uncommon, being detectable in only 3 out of the 22 cases studied. This cytotoxicity did not correlate with clinical staging of the disease but may well have been related to tumour volume. By autoimmunization of patients with an irradiated suspension of their own tumour cells the appearance of cytotoxic lymphocytes could be provoked in 5 out of the 12 patients studied. This cytotoxicity was detectable at the end of the first week after the autograft and disappeared by the third week. Cytotoxic lymphocytes did not correlate in any obvious way with the appearance of specific antitumour antibodies detected by immunofluorescence. So far there has been no evidence of a serum factor capable of blocking the lymphocyte cytotoxicity in these patients.The presence and possible significance of cytotoxic lymphocytes in patients with malignant disease is discussed.


Treatment of myelomatosis--M.R.C. trial.


Heparin-treated haemolytic-uraemic syndrome simulating Fabry's disease.


Venereal disease in women. I.

Venereal disease in women. I.


Clinical trials of therapeutic measures in periodontics.


The design of clinical trials for the assessment of periodontal treatment.


Clinical trials of treatment for acute periodontal diseases.


An evaluation of reparative procedures in periodontal surgery.


The implantation of human dentin and cementum with autogenous bone into the subcutaneous tissues of the rat.


The scanning electron microscope in studies of trabecular bone from a human vertebral body.


Stimulation by nicotine of a vasodilator area on the floor of the fourth ventricle.


Brain acetylcholine levels in rats with septal lesions.


Influence of stannous fluoride on the infrared absorption spectrum of decalcified human dentine.


The great bone case.

The great bone case.


An interview with Dr. Estelle Ramey.

An interview with Dr. Estelle Ramey.


The art of dying.

The art of dying.


Some oddities of medical literature.

Some oddities of medical literature.


Correlation between rate of cell growth and rate of DNA synthesis in Escherichia coli B-r. The rates of cell growth and of DNA synthesis have been measured in synchronized cultures of Escherichia coli B/r growing with three different doubling times, from 27 to 80 min. Cell volume was found to increase linearly over the cell cycle, the rate of increase doubling at a precise cell age for each growth rate. The time at which the rate of cell growth doubled was also found to be the time at which new rounds of DNA synthesis were initiated. However, it was found that these two events could be separated in time by inhibiting DNA synthesis for a short period by the addition of nalidixic acid. It is concluded that neither event is the direct trigger for the other.


Localization of the zinc binding site of aspartate transcarbamoylase in the regulatory subunit. Aspartate transcarbamoylase (EC 2.1.3.2) from Escherichia coli contains six zinc ions per molecule. Renaturation studies of this allosteric enzyme and its isolated subunits show that the metal binding site is in the regulatory polypeptide chain. Ultraviolet difference spectra of the cadmium and zinc derivatives have been used to show that the sites in the isolated subunit and the full enzyme are similar. Results with an apo derivative of the regulatory subunit suggest that the metal is not required for the binding of the feedback inhibitor, but that it is involved in the structural stability of the protein. A close relationship between the reactivity of the cysteine residues in the regulatory subunit and the metal ion has been found.


Localization of a portion of the ribosomal RNA genes in Escherichia coli. About one-half of the genes coding for ribosomal RNA in Escherichia coli have been localized by the use of F-merogenotes. These cistrons have been positioned between 74 and 77 min on the E. coli map described by Taylor and Trotter. The episomal DNA carrying these genes was enriched by chromatography. The map position is discussed with reference to previous localization attempts.


Medical ethics and society. II. Professional co-workers.


Poliomyelitis epidemiology and control.

Poliomyelitis epidemiology and control.


Effect of colicin E3 upon the 30S ribosomal subunit of Escherichia coli. The properties of 30S ribosomal subunits from untreated and from colicin E3-treated (E3-30S) bacteria have been compared. Polyacrylamide gel electrophoresis of ribosomal proteins revealed no difference, but several studies indicated that the 16S RNA from E3-30S particles was modified. E3-16S RNA showed slightly increased resistance to heat-induced degradation and had a reduced (15 S) sedimentation coefficient on sucrose gradients. Fingerprint analyses of E3-16S RNA revealed that the 3'-terminus of the molecule had been deleted. It was concluded that a primary effect of colicin E3 is the activation of a highly specific RNase that degrades 30S ribosomal RNA in situ, and that the resulting fragment(s) are probably retained within the 30S particle.


A pig herd established by hysterectomy. II. Health and performance.


Specific inactivation of 16S ribosomal RNA induced by colicin E3 in vivo. Treatment of sensitive Escherichia coli cells with colicin E3 leads to inactivation of 30S ribosomal subunits. In vitro reconstitution of 30S subunits indicates that the E3-induced defect lies solely in the 16S RNA. 16S RNA from E3-treated cells lacks several T(1) RNase oligonucleotides of normal 16S RNA, including the one from the 3'-end of the 16S RNA, as analyzed by the fingerprint technique of Sanger. An RNA fragment about 50 nucleotides long has been isolated from E3-treated cells. This RNA contains the original 3'-terminal oligonucleotide and other oligonucleotides missing in the E3-16S RNA. The results show that colicin E3 treatment causes the cleavage of 16S RNA at a specific position near the 3'-terminus.


Whose responsibility?

Whose responsibility?


The pathophysiology of ovine ostertagiasis: a study of the changes in plasma protein metabolism following single infections.


Organ transplantation and the law.

Organ transplantation and the law.


Malignancies of the cardiovascular system.


Bovine serum albumin effect on endotoxin-challenged mitochondria.


The Eureka needle.

The Eureka needle.


Accelerated renal allograft rejection following blood transfusion.


The ultrastructure of the mycelium of Streptomyces aureofaciens in the course of biosynthesis of tetracycline.


Behaviour of the gram-negative bacteria in lyophilized tissues of fish meat.


F. Raymond Keating, Jr., Memorial Symposium--hyperparathyroidism, 1970. Dedication.


Intracellular transport of calcium and its relationship to homeostasis and mineralization. An electron microscope study.


Fine structural alterations of parathyroid glands in response to experimental and spontaneous changes of calcium in extracellular fluids.


Recent advances in parathyroid gland pathology.


Clinical applications of radioimmunoassay of plasma parathyroid hormone.


Increase in isoleucine accumulation by alpha-aminobutyric acid-resistant mutants of Serratia marcescens. Several alpha-aminobutyric acid-resistant (Abu-r) mutants of Serratia marcescens were found to be superior to the parent strain in converting d-threonine to l-isoleucine. One of them accumulated 1.5 times more l-isoleucine that the parent strain. The level of acetohydroxy acid (AHA) synthetase in this mutant increased twofold above that of the parent strain. In the parent strain, AHA synthetase was repressed and l-isoleucine accumulation was decreased by either l-valine or l-leucine, whereas in the mutant the AHA synthetase level and l-isoleucine accumulation were not affected by these amino acids. AHA synthetase of the Abu-r mutant was feedback-inhibited by l-valine to the same extent as that of the parent strain. The level of d-threonine dehydratase in both strains was only slightly affected by several amino acids tested. l-Threonine dehydratase of the parent strain and of the mutant was almost completely inhibited by l-isoleucine. These results indicate that the increase in l-isoleucine accumulation by Abu-r mutants is due to the genetic derepression of AHA synthetase.


Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity. Based on the metachromatic property of Toluidine Blue O, three, convenient agar-diffusion methods have been developed that enable detection of the nuclease of Staphylococcus aureus at concentrations as low as 0.005 mug/ml in agar and broth cultures. The interactions of agar and deoxyribonucleic acid with Toluidine Blue O are discussed.


Identification of Mycoplasmatales: procedures for both characterization and purification. Procedures with potential value for identification and purification of Mycoplasma were applied to over 240 cultures representing 11 of the 12 known avian serotypes. Growth was tested at pH 5.5 and 9.5, at 25 and 42 C, in serum-free media, and in the presence of (i) 1% bile salts, (ii) 3% NaCl, and (iii) 0.02% methylene blue. One avian Mycoplasma serotype grew in broth containing 1% bile salts. Two of 11 avian serotypes were resistant to 0.02% methylene blue. A number of Mycoplasma strains grew at 42 C or in a pH 9.5 medium. Usefulness of these procedures for purifying cultures of Mycoplasma is discussed.