Effects of coumarin, thiopurines, and pyronin Y on amplification of phleomycin-induced death and deoxyribonucleic acid breakdown in Escherichia coli. Phleomycin (</=2 mug/ml) induces neither deoxyribonucleic acid (DNA) breakdown nor cell death in stationary-phase Escherichia coli B cells, but the addition of 8 mm caffeine immediately initiates these changes in the same way as increasing the phleomycin concentration 10-fold. This phenomenon is termed "amplification" (6). Pyronin Y, a number of nontoxic thio- and mercaptopurines (of which the most active were 6,7- and 6,9-dimethyl-2-methylthiopurine), and coumarin have been found to be considerably more efficient amplifiers of phleomycin activity than caffeine. Thus 2 mm 6,7- and 6,9-dimethyl-2-methylthiopurine, 0.16 mm pyronin, and 4 mm coumarin killed 10 to 100 times more phleomycin-treated bacteria within 2 hr than 8 mm caffeine. As with caffeine, amplification of cell death by these compounds was accompanied by degradation of DNA to acid-soluble fragments. A number of compounds including 2,6-dichloropurine, 6-hydroxy-2-methylthiopurine, alpha-naphthol, beta-naphthol, naphthionic acid, and alpha-naphthol-4,8-disulphonic acid inhibited the action of phleomycin, if they were present in the cell suspension during phleomycin treatment, but some caused amplification if added subsequent to the phleomycin. Although no mutants resistant to >/=10 mug of phleomycin per ml were observed among 10(11)E. coli B cells screened, such mutants occurred with a frequency of 10(-6) to 10(-7) among cultures resistant to 1 to 2 mug of phleomycin per ml. These double mutants were cross-resistant to phleomycin plus caffeine. The amplifying compounds, though structurally dissimilar, shared the common characteristic of binding selectively to denatured DNA as measured by equilibrium dialysis methods. The implications of these observations in supporting a model of phleomycin amplification proposed previously (6) and their utility in providing a logic for developing a new class of antibiotics are discussed.


Mitomycin C and temperature induction of colicin B in the absence of deoxyribonucleic acid synthesis. An Escherichia coli K-12 strain and its mutant, temperature-sensitive in deoxyribonucleic acid (DNA) synthesis, were used as hosts for two different ColB factors (ColB3-K166 and ColB4-K98). Induction of either colicin occurred in both hosts in the presence of mitomycin C at 42 C. Induction of the temperature-sensitive colicinogenic hosts also occurred without mitomycin C at 42 C at which temperature no DNA synthesis was observed. Colicin synthesis was a nonlethal event in ColB4-K98(+) cultures, whereas in cultures of ColB3-K166(+) induction led to death of a large fraction of the population.


Selection of fimbriate transductants of Salmonella typhimurium dependent on motility. The ability to form type 1 fimbriae (Fim(+)) was readily transduced to 159 out of 161 wild-type motile Fim(-) FIRN strains of Salmonella typhimurium with phage P22 propagated on a Fim(+) donor strain. Fim(+) clones were isolated from about 35% of tests after the fimbriate bacteria in the transduction mixture had been enriched by culture in aerobic static broth for 48 to 96 hr. A Fim(+) transductant was isolated from only 1 out of 280 tests made with 10 nonmotile recipient FIRN strains that were nonflagellate (Fla(-))- or possessed "paralyzed" flagella (Fla(+) Mot(-)), though motile variants from these strains were fully competent in yielding Fim(+) transductants. The property of motility was thought to facilitate the selective outgrowth of Fim(+) transductant bacteria by enabling them to migrate aerotactically to the surface of the broth where their fimbriae permitted them to float and grow in a pellicle stimulated by the free supply of atmospheric oxygen.


New method for extraction of ribonucleic acid and polyribosomes from Schizosaccharomyces pombe. A new method for extracting ribosomal ribonucleic acid (RNA) and polyribosomes from Schizosaccharomyces pombe, a fission yeast, has been developed. Cells grown in the presence of 2-deoxyglucose were found to be more sensitive to the action of Glusulase; a short period of treatment (10 to 20 min) at 0 C was sufficient to convert the cells to osmotically sensitive structures. From these, ribosomal RNA and polyribosomes were readily prepared.


Phenotypic characterization and deoxyribonucleic acid homologies of Pseudomonas solanacearum. Twenty-six strains and colony variants of Pseudomonas solanacearum belonging to four described biotypes were characterized, by using 169 phenotypic characters previously found useful in distinguishing among strains of other Pseudomonas species. Deoxyribonucleic acid (DNA) hybridization (intra- and interspecific DNA-DNA hybridizations) was performed by using the in vitro "DNA competition" technique. P. solanacearum appears to be a moderately homogeneous species, which is, at most, only remotely related to all other species of the genus studied to date. The four biotypes are not clearly distinct from one another with respect to nutritional characters or DNA homologies. Discrepancies between acid production and growth with some carbohydrates were noted. Difficulties were encountered in certain DNA competition experiments and some problems of the methodology are discussed.


New suppresor in Escherichia coli.

New suppresor in Escherichia coli. During the genetic mapping of a mutation in the pheS gene which confers temperature sensitivity on a strain of Escherichia coli K-12, an extragenic suppressor was discovered which restores ability to grow at the restrictive temperature. The suppressor, which has been named supQ, is cotransduced by bacteriophage P1 with the purE marker. SupQ does not suppress a number of amber or ochre mutations. SupQ(-) is carried by the prototrophic Hfr Hayes strain AB259, and the presence of the supQ(-) allele impairs the growth of this strain at 42 C.


Location and orientation of the phoA locus on the Escherichia coli K-12 linkage map. The order of gene loci in the phoA-phoR region of the Escherichia coli K-12 linkage map was demonstrated to be lac phoA proC phoR. The end of the phoA locus corresponding to the amino terminus of alkaline phosphatase was shown to be the end nearer proC. Translation (and transcription) of phoA is therefore in the anticlockwise direction relative to the conventional E. coli linkage map.


Isoleucine hydroxamate, an isoleucine antagonist. Isoleucine hydroxamate (Ile.Hdx) was found to inhibit the growth of Serratia marcescens and to antagonize isoleucine. At a low concentration of Ile.Hdx, at which the growth of the wild strain was completely inhibited, the growth of an isoleucine auxotroph was not inhibited in the medium containing a limiting amount of d-threonine as the isoleucine source. At a higher concentration, this antagonist exhibited a considerable inhibitory effect on the growth of the auxotroph. Ile.Hdx showed the same inhibitory effect as isoleucine on l-threonine dehydratase activity at the concentrations 10 times those of isoleucine. Ile.Hdx caused also derepression of isoleucine-valine biosynthetic enzymes and the derepression was overcome by isoleucine. These results indicate the Ile.Hdx causes growth inhibition by its effects on isoleucine metabolism.


Purification and properties of 3-deoxy-d-arabinoheptulosonic acid-7-phosphate synthetase (phe) from a lambda aroG+ transductant of Escherichia coli. By incorporating aroG, the structural gene for 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase (phe), into the genome of a heat-inducible susR60 mutant of phage lambda, it has been possible to increase the intracellular levels of DAHP synthetase (phe) in a lysogenized strain of Escherichia coli some 15-fold over levels found in the wild-type strain. By using this strain, the enzyme has been purified approximately 2,000-fold compared with wild type, and various kinetic parameters of the purified enzyme have been studied. In contrast to previous reports, the inhibition by phenylalanine was found to exhibit sigmoidal kinetics, suggestive of cooperative interactions between phenylalanine binding sites. Stimulation of enzyme activity by Co(2+) was minimal (14%).


Multivalent repression and genetic depression of isoleucine-valine biosynthetic enzymes in Serratia marcescens. The regulation of the formation of isoleucine-valine biosynthetic enzymes was examined to elucidate the mechanism of isoleucine-valine accumulation by alpha-aminobutyric acid-resistant (abu-r) mutants of Serratia marcescens. In the isoleucine-valine auxotroph, l-threonine dehydratase, acetohydroxy acid synthetase, and transaminase B were repressed when isoleucine, valine, and leucine were simultaneously added to minimal medium. These enzymes were derepressed at the limitation of any single branched-chain amino acid. Pantothenate, which stimulated growth of this auxotroph, had no effect on the enzyme levels. It became evident from these results that in S. marcescens isoleucine-valine biosynthetic enzymes are subject to multivalent repression by three branched-chain amino acids. The abu-r mutants had high enzyme levels in minimal medium, with or without three branched-chain amino acids. Therefore, in abu-r mutants, isoleucine-valine biosynthetic enzymes are genetically derepressed. This derepression was considered to be the primary cause for valine accumulation and increased isoleucine accumulation.


Inactivation of Escherichia coli, F' episomes at transfer, and bacteriophage lambda by psoralen plus 360-nm light: significance of deoxyribonucleic acid cross-links. We have investigated some biological consequences of light-induced psoralen-deoxyribonucleic acid (DNA) adducts and find that for several Escherichia coli functions (killing of strain AB2480 recA13 uvrA6, inactivation of phage lambda plaque-forming ability in wild type and uvrA6 hosts, loss of ability to transmit intact Flac(+) episomes), a light exposure sufficient for production of a single cross-link per DNA molecule correlates well with the biological consequence. Although one cross-link per genome is apparently lethal to recA13 uvr(-) strains, mutants carrying the recA13 or uvrA6 markers survive light exposures producing 6.7 and 16 cross-links per genome, respectively, and wild-type cells recover from 65 psoralen cross-links. Evidently, the excision and recombinational repair systems complement one another in reconstructing an intact genome from cellular DNA containing psoralen photoproducts. The above bacterial and phage strains, in which DNA repair processes are minimized, are also extremely sensitive to pyrimidine dimer-forming 254-nm UV light (without psoralen), and were expected to respond similarly to formation of psoralen-pyrimidine base monoadducts in their DNA. Since the biological inactivation by psoralen correlates well with cross-link formation, we suggest that the sensitizing action of this drug primarily derives from its ability to form DNA cross-links.


Obituary: John A. Geraghty.

Obituary: John A. Geraghty.


Transport-limited growth rates in a mutant of Escherichia coli. Mutants of Escherichia coli B/r have been selected which require increased nutrient concentrations for half-maximal growth rate. This half-saturation constant (K(m)) for growth on glucose is 10(-6) and 7 x 10(-4)m for the wild type (CP 366) and the mutant (CP 367), respectively. Similarly, the K(m) is increased for growth on many other carbohydrates (20- to 500-fold), for the anions PO(4) (3-) and SO(4) (2-) (ca. 100-fold), and for the uptake of several amino acids (20- to 50-fold). At sufficiently high concentrations of the nutrients, mutant and wild type grow equally fast. The yield in terms of cell mass per milligram of substrate is unaffected by the mutation. The phenotype of the parent is reestablished in what appears to be the reversion of a single mutation (kmt) which maps between strA and metB. The pleiotropic decrease of the affinities for transport of the various nutrients seems to be the result of a modification of the cell envelope which weakens the attachment of the various specific binding proteins to the periplasmic membrane. Since the mutant K(m) values are increased considerably, high cell densities can be reached in batch cultures at growth-rate-limiting substrate concentrations (10(7) to 10(8) cells/ml). This allows chemical analysis of the cell composition; the application of the mutant to studies of bacterial physiology as function of growth rate is discussed.


"Phospholipids of virus-induced membranes in cytoplasm of Escherichia coli. Infection of Escherichia coli with amber mutants of phage fd, in contrast to infection with wild-type phage, leads to cell death and the proliferation of intracytoplasmic membranes observed in electron micrographs at the poles of the cells. The accumulation of membranes correlates with changes in structural phospholipids, especially a marked increase in the apparent rate of formation and total amount of cardiolipin (from 4 to 20% of total radioactive phospholipids), and a compensating decline in phosphatidylethanolamine.


New therapeutic agents for peptic ulcer.


Dr. Anson Gordon Smythe: member first Mississippi State Board of Health.


Regulation of glycolysis in the ischemic and the anoxic myocardium.


Trivalent combined measles-mumps-rubella vaccine. Findings in clinical-laboratory studies.


Carbenicillin therapy for pseudomonas infections.


Chemistry of phospholipids in relation to biological membranes.


The development of bioorganic chemistry in the U.S.S.R. during the last decade.


Recent experiences in the respiratory unit of the Johannesburg Hospital.


Fifty years of insulin.

Fifty years of insulin.


Inherited metabolic disease and the eye.


Paul Bert and the beginnings of aviation medicine.


A new inexpensive IPPV exhalation valve for automatic ventilators.


Lamellar keratoplasty. Malbran's "peeling off" technique.


Lymphomatous leptomeningitis.

Lymphomatous leptomeningitis.


Corneoscleral lamellar transplant for recurrent pterygium.


Disappearance of intravitreous  133 xenon. Its relation to ocular blood flow.


The man and the knife.

The man and the knife.


Introduction of the first Carl G. Hartman lecturer.


Intravaginal relocation of an ovary in the ewe: surgical technique and initial observations.


Hypothermic perfusion preservation of the canine kidney with a simulated intracellular fluid.


Sacral nerve stimulation. A diagnostic test of bladder innervation.


Weir Mitchell's address on instrumental precision in medicine in the perspective of 70 years.


Postmortem bacteriology.

Postmortem bacteriology.


Activity pattern of visceral cortex neurons during asphyxia.


Electrophysiological properties of units of the thalamic reticular complex.


Salmonella hybrids containing genic material of multiple origins.


Professor Erwin H. Ackerknecht. Birthday tribute.


Interaction of a colicinogenic factor with a resistance factor and with the fertility factor F in Escherichia coli K-12.


Conditional induction of lambda-prophage in exrA mutants of Escherichia coli.


Note for the index of recorded copies of William Harvey's De motu cordis. The second or 1635 edition.


Medical inspection of prostitutes in America in the nineteenth century: the St. Louis experiment and its sequel.


Insecticide spray residues and public health: 1865-1938.


The end of Greek diet.

The end of Greek diet.


Patrick Clarkson, 1911-1969.

Patrick Clarkson, 1911-1969.


Ralph H. Major 1884-1970.

Ralph H. Major 1884-1970.


Citation for Paul Louis Ziemer.

Citation for Paul Louis Ziemer.


The speed of suppression by iodate of thyroid iodine uptake.


Initial evaluation of a subjective Bayesian diagnostic system.


An immunopathologic study of rapidly progressive glomerulonephritis in the adult.


Human renal allografts. Glomerular deposits of horse immunoglobulin G and nephritis following administration of antilymphocyte globulin.


Kidney diseases. Forward.

Kidney diseases. Forward.


Strontium chloride B and E.E. enrichment broth media for the isolation of Edwardsiella, Salmonella and Arizona species from tiger snakes. Strontium chloride B medium and E.E. broth have been found effective in the recovery of Edwardsiella, Salmonella and Arizona species from the cloacal contents of tiger snakes (Notechis scutatus). Strontium chloride B medium was superior to E.E. broth.At least one bacterial species was detected in each of the 60 reptiles examined, and all three organisms were recovered from each of 29 snakes on a single examination.Strontium chloride M, strontium selenite and Rappaport enrichment media and bismuth sulphite agar, although satisfactory for the isolation of Salmonella and Arizona species, were found unsuitable for Edwardsiella tarda.


Beta-haemolytic streptococci in saliva.

Beta-haemolytic streptococci in saliva. Viable counts of beta-haemolytic streptococci per ml. of saliva were made in the following groups: (1) children with acute streptococcal sore throat, (2) children with acute non-streptococcal sore throat, (3) children who had no sore throat but were streptococcal throat carriers, (4) children who neither had a sore throat nor were streptococcal throat carriers.The mean counts from cases of streptococcal sore throat and from streptococcal carriers were respectively 1.4 x 10(6) and 2.5 x 10(5) per ml.In a comparison of the efficiency of the throat swab, sublingual swab and specimen of saliva in isolating beta-haemolytic streptococci from the upper respiratory tract, culture of saliva produced the best results.


Bacteriological monitoring in penicillin treatment of streptococcal sore throat. Bacteriological monitoring of penicillin therapy in 30 children with streptococcal sore throats was performed by means of salivary and throat-swab culture, on the first, third, fifth and ninth days after therapy had started. Counts of beta-haemolytic streptococci per ml. of saliva were also performed.Results showed that salivary culture and estimation of the numbers of beta-haemolytic streptococci in the saliva were much more sensitive indices of the effectiveness of penicillin treatment than throat-swab culture.


The intestinal flora of colonization-resistant mice. To facilitate the isolation of anaerobes from cultures in which swarming organisms were present Roux flasks were adapted to provide anaerobic conditions. Using these flasks, five species of anaerobes were isolated from the caecum of mice resistant to colonization with Escherichia coli and other Gram-negative organisms. These species accounted for the majority of organisms seen in films of caecal material. No aerobes were isolated and the flora of these mice apparently consisted of a limited number of anaerobes representing part of the intestinal flora of conventional mice. Three of the species were clostridia and another was identified as Propionibacterium acnes. One species resembled Catenabacterium but was not identified. One of the Clostridium species could only be isolated when the medium was supplemented with yeast extract and vitamins. This species sporulated on media with ammonium phosphate as the source of nitrogen; tryptone completely suppressed sporulation.


Salmonellosis in Botswana. I. Incidence in cattle. Clinical salmonellosis in cattle is infrequently recorded in Botswana, the majority of salmonella isolations being made from apparently healthy animals. A salmonella carrier rate of 6.7% was found in testing faeces samples from 89 apparently healthy cattle. A total of 35 salmonella serotypes were isolated from specimens of bovine origin. Salmonella dublin was isolated infrequently, possibly in part because of prophylactic vaccination.


Salmonella typhimurium contamination of processed broiler chickens after a subclinical infection. A subclinical infection of Salmonella typhimurium in a broiler flock was investigated and attempts were made to eradicate the infection by treatment with furazolidone. One-quarter of the chickens were still infected after they had been through the processing plant. Washing in heavily chlorinated water reduced the number of contaminated carcasses. Infected chickens were also found in four other companion flocks on the same farm.


An integrated service for the treatment of irreversible renal failure.


Common problems in clinical trials.

Common problems in clinical trials.


A survey of snowmobile injuries and hazards.


The biology of aging.

The biology of aging.


Evaluation of islet-cell function in man.


Sequence relationship of three valine acceptor tRNAs from Escherichia coli.


Nature of DNA precursors.

Nature of DNA precursors.


Note on the validity of Machover's indicators of anxiety.


Cross-cultural study of relationships among four dimensions of time perspective.


Perceptual field dependence among aged persons.


Comparative study of tachistoscopic perception of binary figures in deaf children and normally hearing children.


Relationships among Wechsler adult intelligence scale, Goodenough-Harris, and Peabody picture vocabulary tests with institutionalized retarded adults.


Arizona isolation using selenite broths with altered selenite contents incubated at 35 and 43 degrees C.


Medicine and manuscripts. Tobias Smollett, M.D. (1721-1771).s.


College students as therapeutic agents.

College students as therapeutic agents.


Psychophysiological approaches to the investigation of desensitization and related procedures.


Test anxiety and direction of attention.


The University of Texas Medical School at Houston: a beginning.


Cross-matching for organ transplantation. I. The use of kidney cells and immune adherence.


Relation of canine histocompatibility testing to marrow grafting.


Net histocompatibility ratios (NHR) for clinical transplantation.


Histocompatibility requirements of heart and skin grafts in rats.


Determination of histocompatibility antigens by leukocyte typing in outbred guinea pigs and effect of matching on skin graft survival.


Corneal transplantation and histocompatibility in rabbits.


Detection of weak histocompatibility antigens on mouse peritoneal macrophages.


Modified antigenicity: an immunologic mechanism for species survival.


Effects of diverse tissue implants on the survival of subsequent skin allografts transplanted across weak histocompatibility barriers in newts and mice.


Conditioning of allogeneic mice with crude and purified H-2 extracts, alone and combined with cyclophosphamide, for skin graft prolongation.


Role of preservation in clinical renal transplantation.


Diagnosis and management of rejection in allografted kidneys.


Immunosuppression by chemical agents.

Immunosuppression by chemical agents.


Structure and function of the Organ Transplant Registry of the American College of Surgeons.


Transplant survival and patient activity data from the Human Kidney Transplant Registry.


Current state of lung transplantation.

Current state of lung transplantation.


Current state of pancreatic allotransplantation.


Results of human heart transplantation at Stanford University.


Current state of clinical heart transplantation.


Renal transplantation in patients with Australia antigenemia.


Characteristics of erythropoiesis following human renal homotransplantation.


Evaluation of cadaver-donor renal transplantation.


Renal transplantation in infants and small children.


Recurrence of an original glomerular lesion in three renal allografts.


Immunological studies of proteinuria following human kidney allotransplantation.


Histocompatibility concomitants of the clinical course of renal homografts.


Computer storage and use of medical records of human renal allotransplants.


Cellular interaction between thymocytes and transplanted marrow stem cells.


Diagnosis of chronic rejection after cardiac transplantation in humans.


Extracorporeal irradiation of the blood: lymphocyte transformation tests and clinicl results after renal transplantation.


Ability of normal bone marrow to restore second-set immunity.


Immunofluorescent and serologic studies of the humoral antibody response to cardiac allotransplantation in man.


The postoperative transplant patient.

The postoperative transplant patient.


in vitro quantitation of lymphocyte and serum cytotoxic activity following renal homograft rejection in man.


Correlation of platelet aggregation in canine renal allotransplants with serum cytotoxic antibody following treatment with azathioprine.


Lymphocyte activation during allograft rejection.


Role of passenger leukocytes in the rejection of renal allografts.


Further analysis of the mechanism of prolonged survival of canine renal allografts.


Effects of retransplantation of canine kidneys into original donor after 3-day implantation into randomly selected homologous host.


Rejection of renal and splenic autografts.


Prevention of rejection of porcine renal allografts.


Delayed recovery from acute tubular necrosis following radiation.


Experimental orthotopic transplantation of the pancreas.


Morphological features of kidney and liver allografts in the pig.


Functional alterations in pig liver allografts.


Heart graft arteriosclerosis.

Heart graft arteriosclerosis.


Advances in lung transplantation.

Advances in lung transplantation.


Antibodies in the serum and on the heart of dogs with cardiac allografts.


Feasibility of simultaneous bilateral lung replantation.


Relationship between structural and functional changes in rejecting renal xenograft.


Xenograft rejection by dog and man: isolated kidney perfusion with blood and plasma.


Modification of xenograft response by selective plasmapheresis.


Prolongation of renal xenografts by the simultaneous sequestration of preformed antibody, inhibition of complement, coagulation and antibody synthesis.


Modification of hyperactue xenogeneic kidney rejection.


Cardiac and skin heterograft rejection: suppression with antilymphocyte serum.


Rejection phenomena in heterotopic liver homo- and heterografts.


Effect of heparin, arvin, liver perfusion, and heterologous antiplatelet serum on rejection of pig kidney by dog.


Allogeneic "rejection" of in situ rat kidney using humoral antibody.


Humoral factors in experimental renal allograft and xenograft rejection.


Factors contributing to survival of rats transplanted with kidneys mismatched at major locus.


Prolongation of rat heart allografts by donor pretreatment with immunosuppressive agents.


Lung transplantation in the rat.

Lung transplantation in the rat.


Age limit for the use of syngeneic donor kidneys in the rat.


Studies on cortical necrosis in renal grafts.


Immunology of anterior chamber of the eye.


Successful 24-hour kidney preservation with simplified hyperosmolar hyperkalemic perfusate.


Assessment of renal function during isolated in vitro perfusion.


Hyperbaric kidney preservation with gaseous media.


Successful 18- to 23-hour canine and human kidney preservation.


Perfusate composition in organ preservation.


Successful transportation of kidneys between distant centers in North America.


Viability, function and transportation of preserved canine kidneys.


Reliable method for preservation and transportation of donor heart.


Experimental organ preservation.

Experimental organ preservation.


Studies of preservation of liver and pancreas by freezing techniques.


Successful orthotopic liver transplantation after preservation by simple hypothermia.


Unresponsiveness to skin allografts induced by tissue extracts and antilymphocytic serum.


Host acceptance of well-matched kidney allograft.


Active enhancemant of renal allografts.

Active enhancemant of renal allografts.


Specific inhibition of organ allograft rejection by donor blood.


Active enhancement of renal allografts in dog and rat by subcellular antigen.


Passive enhancement of skin heterografts.


Hematopoietic origin of immunological target in enhancement of renal allografts.


Evaluation of intravenous route for administration of antilymphocyte globulins in humans.


Induction of tolerance to antilymphocyte globulin in man.


Clinical experience with antilymphocyte globulin (ALG) in renal transplantation.


Controlled clinical trial of antilymphocyte globulin in human renal allografts.


Trial of rabbit antihuman ALG in cadaver kidney transplantation.


Use of minimal doses of lymphoid cells for production of heterologous antilymphocyte serum.


De novo malignant tumors in organ transplant recipients.


Complications in prolonged administration of antilymphocytic sera.


Prolonged inhibition of homograft rejection after cycloleucine.


Allograft prolongation with synthetic progestins. Experimental evidence that medroxyprogesterone acetate (MPG) prolongs survival of rabbit skin allografts and the survival of dogs with renal allografts has been reported previously.  Reviewed in this study are additional experience with MPG and evaluation of melengestrol acetate (MGA), a more potent progestin.  Skin grafts were exchanged between New Zealand white and California rabbits of both sexes.  Recipients were treated with intramuscular injections of MPG, 50 mg/ml; MGA, 50 mg/ml; estradiol cypionate, 2 mg/ml; horse antirabbit thymocyte globulin (ALG); or combinations of these agents.  MPG or MGA significantly prolonged survival of rabbit skin allografts.  Large doses of MPG also prolonged survival of dogs with renal allografts, but MGA did not.  Rabbit skin allografts survival was further enhanced when MPG was combined with estradiol or when suboptimal doses of MPG and MGA were combined with a suboptimal dose of ALG.  When MPG or MGA were combined with azathioprine, a marked prolongation on survival of dogs with renal allografts was observed.  In a preliminary trial of MPG in humans with intrafamilial renal transplants, chronic slow rejection was not halted by addition of MPG to the immunosuppressive regimen.  In patients whose renal function was stable at the time of initiation of MPG therapy, prednisone dosage could be lowered to an average of 37% of the previous dose and the patient's Cushingoid appearance or aseptic necrosis of the hips either did not progress or improved.


Effect of incubating skin in steroid suspension (triamcinolone acetonide) on survival of skin allografts in rabbits.


L-asparaginase-induced inhibition of transplantation immunity: prolongation of skin allografts in enzyme-treated mice.


Immunosuppressive effect of cell-free extracts from Escherichia coli.


Prolonged renal allograft survival: extracorporeal irradiation of blood.


A sensitive assay for transplantation immunity in mice.


Prolonged skin allograft survival achieved by in vivo cooling.


Rejection of skin allografts by irradiation chimeras: evidence for skin-specific transplantation barrier.


Host repopulation of endothelium.

Host repopulation of endothelium.


Sensitivity of mixed lymphocyte reaction and its correlation with skin graft survival.


Relevance of mixed lymphocyte reactions to renal allograft rejection mechanisms.


In vitro studies on the mechanism of hyperacute xenograft rejection.


Cluster-forming cells and response to skin allografts.


Controlled, double-blind trial of antiplatelet aggregating agents in vascular renal allograft rejection.


Cross-reactions in mammalian transplantation with particular reference to streptococcal antigens and antibodies.


Inhibition of allograft reaction by biologically filtered antigens.


Heart-reactive antibody: a monitor of cardiac rejection.


Mixed leukocyte cultures in transplantation immunology.


Host cell membrane-associated phage phi X174 DNA replication. I. Isolation and partial characterization of phi X DNA-containing complexes released from the membrane.


Mutants of T2gt with altered DNA methylase activity: relation to restriction by prophage P1.


Genetic distances separating adjacent base pairs in bacteriophage T4.


Genetic changes within prophage phi 80 correlated with exchanges between Lost markers on either side.


Lambda lysozyme synthesis in the absence of N protein.


Aberrancy: electrophysiologic aspects.

Aberrancy: electrophysiologic aspects.


A simple technique for detecting small defects of the atrial septum.


Augustus Volney Waller on blood vessels and inflammation. I.


Behavioral and developmental studies of visual depth perception.


Colicin types of Salmonella enteritidis strains isolated in the Wroclaw province.


Preliminary studies on the influence of the composition of the culture medium and incubation temperature on propagation of Shigella flexneri phage X.


Transfer of aerocinogeny in Enterobacter aerogenes.


Trials of transduction with defective Shigella flexneri phage.


Indirect migration inhibition test in xenogeneic systems. 3. Cellular immunity to kidney grafts.


Immunologic response of AKR mice in different phases of development of transplanted gross leukemia. II. Comparative investigation of cellular and humoral antibody formation.


Johnes bacillus and mycobactins.

Johnes bacillus and mycobactins.


Experimental transmission of pneumonia in sheep with a mycoplasma isolated from pneumonic sheep lung.


Some factors affecting the distribution and rate of action of insecticides. The amount of insecticide that reaches a critical site of action within an insect is affected by a number of physical and chemical processes both within and outside the insect. This paper reviews some of these processes, with particular attention to those external to the insect, whose action commences at the moment an insecticide is released into the environment. On the basis of research that has been conducted on such processes, conclusions are drawn as to the properties that are desirable, from the point of view of insecticidal efficiency, in new compounds.


Toxicity of carbamates to insects.

Toxicity of carbamates to insects. The discovery of effective insecticidal materials among the carbamic-acid esters is surveyed to provide insight into some of the factors leading to the development of new control agents. Problems associated with attempts to correlate insecticidal activity with acetylcholinesterase inhibition are outlined. The intoxication syndrome is described and its toxicological significance discussed. Structure-activity profiles are presented for selected compounds to illustrate the biological effects produced by structural variations. Categories of carbamates in which no significant insecticidal activity has been discovered are also listed.


Biological and nonbiological modifications of organophosphorus compounds. The general types of biological reaction that are most prominent in the modification of organophosphorus compounds involve the mixed-function oxidases, hydrolases, or transferases. In certain cases, more than one of these reactions may be involved at the same site on the pesticide molecule. Examples of various organophosphorus pesticides that are altered by oxidation, hydrolysis, alkyl- or aryl-group transfer, reduction, and conjugation are discussed. The increase or decrease in toxicity of a pesticide that can result from biological modification is emphasized.Non-biological transformations of organophosphorus compounds involve the effect ou the compounds of such factors as light, air, temperature, and solvent. These factors are discussed with special emphasis on desulfuration, rearrangement, and oxidation.


The relationship between the structure and the activity of pyrethroids. There is considerable scope for developing new non-persistent insecticides with little hazard for man and mammals by modifying the structures of the natural pyrethrins. New compounds already synthesized are more effective against some insect species than are the natural compounds, are even less hazardous to mammals, and do not need synergists to supplement their insecticidal action. Other examples show considerable insect species specificity. These compounds may help to control insect vectors when other insecticides are no longer effective because resistance has developed or because their residues can no longer be tolerated.


Insect hormones and their derivatives as insecticides. The hormonal control of moulting, reproduction, and diapause in insects has little or no relationship to any similar phenomena in other animals, and the hormones involved in these processes are unlike any known hormones of vertebrates.The availability of pure chemicals with high biological activity has permitted an astonishing increase in research on insect hormones. At present, understanding of insect endocrinology is far too incomplete to justify much speculation about the possibility of using insect hormones as insecticides. However, the preliminary studies discussed in this paper give reason for hope, and the results justify further effort.


Comparative aspects of the purification and properties of cholinesterases. Recent years have seen great progress in the purification and characterization of cholinesterases. Investigation has indicated the existence of two principal groups: a fairly homogeneous group of acetylcholinesterases and a group of enzymes that utilize butyrylcholine, propionycholine, or benzoylcholine as substrates and that differ widely in their properties.This paper reviews the different types of cholinesterase and their sources, the importance of a proper choice of substrate in cholinesterase studies, methods for the purification of cholinesterases, and some of the properties of these enzymes.


Effect of Bacillus Calmette-Guérin on mammary tumor formation and cellular immunity in dimethylbenz(a)anthracene-treated rats.


Ferritin-labeled antibody studies of feline C-type particles.


Science, food and man's future.

Science, food and man's future.


The effect of copper sulphate on pituitary LH in rats.


L-threo-Neopterin, the major pterin in Escherichia coli B.


The amino acid substitutions found in the genetic variants of   1 -acid glycoprotein.


The mechanism of amino acid control of guanylate and adenylate biosynthesis.


Utilization and transport of hexoses by mutant strains of Salmonella typhimurium lacking enzyme I of the phosphoenolpyruvate-dependent phosphotransferase system.


Sacrococcygeal pilonidal sinus.

Sacrococcygeal pilonidal sinus.


Modification of Escherichia coli glutamate transfer ribonucleic acid with bisulfite.


Review of the literature on diseases of the colon and rectum, March-April, 1970.


Grain prices and vital statistics in a Portuguese rural parish, 1671-1720.


Specificity of AAG codon recognition by lysyl transfer ribonucleic acid from yeast.


Dr. Med. H.C. Wilhelm Hillebrand by K.G. Ober, Geburtsh, Frauenhelik. (1920) 20, 188.


Factors influencing the production of staphylococcal enterotoxin A in milk.


Regeneration in the sea cucumber Leptosynapta. II. The regenerative capacity.


Prelethal effects of killing action by stock 7 of Paramecium aurelia.


A review of clinical renal transplantation at the University of Alabama Medical Center.


Karl Thomas--a biographical sketch (1883-1969).


Chicago rehabilitation facilities.

Chicago rehabilitation facilities.


The future of rehabilitation--who will lead? by Frederic G. Elton, Rehabilitation Review, 1927.


Interpretation of plasma bilirubin levels based on studies with radioactive bilirubin.


Cesare Bressa (1785-1836) on dirt eating in Louisiana: a critical analysis of his unpublished manuscript, De la Dissolution Scorbutique.


FTA-ABS test sensitivity.

FTA-ABS test sensitivity.


The two faces of pathogenic Escherichia coli.


Robert Dick.

Robert Dick.


Anthony Ian Ryan.

Anthony Ian Ryan.


The scourge of rubella.

The scourge of rubella.


Rubella vaccination trial.

Rubella vaccination trial.


Harry Wyatt Wunderly.

Harry Wyatt Wunderly.


A gene controlling the early development of protoperithecium in Neurospora crassa.


Increased recombinant frequencies with an amber mutation in the gal-operon of E. coli.


Amplification of phleomycin induced death and DNA breakdown by caffeine in Escherichia coli.


5-BU sensitization to UV-induced killing and mutability in hcr +  and hcr -  strains of Escherichia coli.


Mutagenesis of bacteriophage T 4  by a carcinogen,  4 -nitroguinoline I-oxide.


The photodynamic inactivation of   bacteriophage particles in the presence of methylated proflavine.


T-strains of mycoplasma in bronchopneumonic lungs of an aborted fetus.


Gonioscopy after cataract extraction.

Gonioscopy after cataract extraction.


Metastatic neoplasms to the head and neck.


Experimental infection of newborn piglets and weanling pigs with haemolytic E. coli, strain A 1 , serotype 0149:K91:H10.


A literary review on bovine fluorosis.

A literary review on bovine fluorosis.


Immunofluorescent immunoglobulin differentiation of vibrio fetus antibodies from bovine cervico-vaginal secretions.


Optometric education and public health.

Optometric education and public health.


Cellulase production by Thermomonospora curvata isolated from municipal solid waste compost. A cellulolytic, thermophilic actinomycete (previously isolated from municipal refuse compost samples) was identified as Thermomonospora curvata. A determination was made of the optimal conditions for cellulase production by T. curvata when grown at 55 C in a medium containing mineral salts, cellulose, and yeast extract. The pH and temperature optima (pH 6.0 and 65 C) for the cellulase produced by T. curvata were identical to those previously observed for the cellulase extracted from crude compost samples. Such similarities, together with the prevalence of T. curvata in compost samples and its ability to grow at composting temperatures, indicate that this actinomycete could possibly be considered as a major cellulose decomposer in the municipal refuse composting process.


Endotoxin-induced disseminated intravascular coagulation in nonpregnant rats. A new experimental model. Disseminated intravascular coagulation as indicated by glomerular capillary thrombosis was induced in unprepared virgin rats by the infusion of endotoxin. Dose-time curves revealed that the minimal amount necessary was 0.9 mg and the time required was 3 hours. A marked difference in susceptibility between the summer and winter seasons was observed, the animals being more sensitive in the former. The platelet count decreased in a dose-related manner; however, there was no difference between an infusion and injection regimen. There was no difference in platelet numbers between the animals who had fibrin deposits and those free of fibrin. Plasma hemoglobin increased by 500% in those animals who developed glomerular fibrin deposition and the hematocrit decreased less profoundly. The data demonstrate that unprepared virgin rats can be used as an experimental model for studying glomerular fibrin deposition, the hallmark of what is generally referred to as the generalized Shwartzman reaction, if endotoxin is continuously infused rather than injected.


Stimulative effect of elemental sulfur on siomycin production by Streptomyces sioyaensis. The addition of elemental sulfur to the fermentation of Streptomyces sioyaensis in a soybean meal medium resulted in a three- to fourfold increase of siomycin. Further experiments on the effect of elemental sulfur during fermentation suggest that one of the key steps stimulating siomycin synthesis is the utilization of thiosulfate, which accumulates in the medium as the result of the oxidation of elemental sulfur.


Isolation of psychrophilic bacteriophage-host systems from refrigerated food products. Thirty-eight bacteriophage-host systems were isolated from 22 of 45 refrigerated food products examined under psychrophilic conditions. Isolates were obtained from ground beef, pork sausage, chicken, raw skim milk, and oysters, whereas no isolations were made from liquid egg whites and processed meat products. Thirty of the 38 psychrophilic bacterial hosts were gram-negative rods, and 27 of these were classified within the genus Pseudomonas; three were members of the family Enterobacteriaceae. The remaining eight were gram-positive cocci, which were tentatively classified as Leuconostoc. Plate counts of psychrophilic bacteria were greater than 2.2 x 10(5)/ml (g) in all but one sample which contained phage, whereas phage titers ranged from less than 100 to 6.3 x 10(6) plaque-forming units/ml (g). Phage isolates showed limited host ranges usually attacking only those hosts upon which they were isolated. Of eight phages tested against 13 cultures of known identity, one showed lytic action, and this was against strains of P. fragi.


Effect of rehydration on recovery, repair, and growth of injured freeze-dried Salmonella anatum. From 70 to 90% of the Salmonella anatum cells that survived freeze-drying in nonfat milk solids were injured. After rehydration, these injured survivors failed to grow on a selective plating medium containing deoxycholate but could form colonies on a nonselective medium. In a suitable environment after rehydration, injury disappeared in most of these cells. The rate of this repair at 25 C was very rapid initially and, in a medium containing milk solids, was completed within 1 hr after rehydration. The repaired cells initiated growth about 1 hr later than normal cells and grew at a slower rate. In a medium containing milk solids, initial recovery, extent of repair of injury, initiation of growth, and rate of growth were not influenced by supplementation with extra nutrients in other rehydration media. Rehydration controlled by modifying the concentrations of lactose, sucrose, or milk solids in the rehydration medium influenced the recovery of cells and the time that growth was initiated. Glycerol failed to increase recovery. Higher numbers of cells were recovered by rehydrating at 15 to 25 C, but an earlier initiation of growth and more rapid growth were observed at 35 C.


Corticosteroids and the generalized Shwartzman reaction. Mechanisms of sensitization in the rabbit. An analysis of the effects of adrenal cortical steroids on the response of the vascular system to bacterial endotoxin has shown that glucocorticoids (cortisone, triamcinolone) are capable of preparing the system for the generalized Shwartzman reaction. On the other hand, the mineralocorticoid desoxycorticosterone acetate does not have this capability. The mechanism of preparation by glucocorticoids has been studied. Administering insulin, with maintenance of normal blood sugar, had no effect on the extent of glomerular capillary thrombosis. Blockade, however, of alpha-adrenergic receptor sites by Dibenzyline caused a significant reduction of the thrombosis. In addition, cortisone-treated animals did not require exogenous stimulation of alpha-adrenergic receptor sites by norepinephrine to localize thrombi in the glomerular capillaries when Hageman factor was activated (by ellagic acid) and fibrinolysis inhibited (by epsilon-aminocaproic acid). It is concluded that glucocorticoids prepare for the generalized Shwartzman reaction by increasing the sensitivity of the microcirculation to stimulation of alpha-adrenergic receptor sites.


Improved solid medium for the detection and enumeration of pectolytic bacteria. An improved solid agar medium (MP medium) has been developed which allows detection of pectolytic activity in bacteria. Organisms tested exhibited a variety of regulatory controls governing pectate lyase synthesis. The medium contains mineral salts, pectin, and yeast extract. After growth of the organisms, the agar plate is flooded with a polysaccharide precipitant, and pectolytic activity is shown by clear zones around active colonies. High concentrations of phosphate are shown to be necessary for pectic enzyme formation on solid media. The medium has successfully been used to detect pectolytic organisms in soil, forest litter, and rotting vegetable samples.


Action of Pseudomonas fragi on the proteins of pig muscle. Considerable salt-soluble protein degradation was observed in pork muscle inoculated with Pseudomonas fragi. During a 20-day incubation period at 10 C, the samples proceeded to rank spoilage or putrefaction. There was a large decrease in the salt-soluble protein fraction and a corresponding increase in nonprotein nitrogen. Disc gel electrophoretic patterns showed that breakdown of the salt-soluble proteins had occurred after incubation for 20 days. During incubation for 10 days at 10 C, P. fragi produced large amounts of extracellular proteolytic activity in ground pork. Most of the proteolytic activity appeared immediately after spoilage occurred. However, a significant increase in the ability to hydrolyze casein and a slight increase in the ability to hydrolyze denatured hemoglobin occurred prior to spoilage.


Microbial flora of pecan meat.

Microbial flora of pecan meat. Microorganisms found associated with commercially shelled pecan meats are numerous and varied. No bacteria and only two fungal microorganisms, Aspergillus clavatus and Tricothecium species, were found on aseptically shelled pecans.


Changes in Escherichia coli associated with acquired tolerance for quaternary ammonium compounds. Acquired tolerance for a quaternary ammonium compound produced a tolerance for a similar compound. Tolerance was associated with the structure and the extent of adsorption of the compound. Morphological changes and resistance to disruption by pressure and by sonic treatment accompanied the development of tolerance. An otherwise weakened culture evolved with the acquisition of tolerance. The maximum obtainable viable population density of tolerant cells in growth medium was approximately 5% of that obtained in the parent culture. Tolerant cultures died off more rapidly in the original growth medium as well as when washed cell suspensions were stored at 5 C. Since acquired tolerance was associated with an otherwise weakened culture, the occurrence of the tolerant cells to limit the efficacy of quaternary ammonium compounds in sanitation operations is highly unlikely.


Effects of resistive exercise on muscle size: a review.


Results of treatment of amblyopia due to central corneal apacities by partial penetrating keratoplasty and occlusion of fixing eye in children of 3 to 5 years of age.


Ribonucleic acid synthesis in animal cells in the presence of actinomycin.


The effect of immunosuppressive agents on the corneal graft rejection.


Studies on the activity of the exophthalmos-producing substance (E.P.S.). II. Lack of suppressibility by thyroxine.


New concepts in the treatment of the dendritic ulcer.


Acid injury of Escherichia coli.

Acid injury of Escherichia coli.


Influence of growth of Streptomyces fradiae on pepsin-HCl digestibility and methionine content of feather meal.


Physiology of sporeforming bacteria associated with insects. 3. Radiorespirometry of pyruvate, acetate, succinate, and glutamate oxidation.


Suppression of dual-effect mutants of the L-ribulokinase structural gene of Escherichia coli B/r.


A simple method for enumeration of antibiotic-producing microorganisms in the rhizosphere.


Traditions and trends in nursing.

Traditions and trends in nursing.


The hemolytic anemias of alcoholic liver disease.


Weir Mitchell's address on instrumental precision in medicine in the perspective of 70 years.


Kidney transplantation: a progress report.


Malaria revisited.

Malaria revisited.


Content and activity of insulin in the blood and the sensitivity of tissues to it during aging.


Diagnostic nets.

Diagnostic nets.


Computer stimulation of a living cell. I.


The effects of posture and isoproterenol on the velocity of left ventricular contraction in man. The reciprocal relationship between left ventricular volume and myocardial wall force during ejection on mean rate of circumferential shortening. A study was performed in five normal men in whom left ventricular volume was measured by thermodilution in the supine and 60 degrees head-up postures, in the control state, and then during steady-state response to isoproterenol. The mean rate of circumferential shortening of the left ventricle was calculated for each of the postures in both inotropic states and was found to remain constant in the control state at 12.5 +/-0.6 cm/sec in the supine posture and 13.3 +/-0.5 cm/sec in the tilted posture. Similarly, mean rate of circumferential shortening remained constant in response to the positive inotropic effect of isoproterenol at 20.9 +/-0.5 cm/sec in the supine position and 20.7 +/-0.5 cm/sec in the tilted posture. It is concluded that the constancy of mean rate of circumferential shortening over the relatively broad physiologic range of left ventricular end-diastolic volume and mean force of ejection during a given state of myocardial contractility represents the coupled reciprocal influences of ventricular wall tension and myocardial fiber length on the velocity of ventricular wall shortening. Unlike stroke work, stroke power, and mean rate of left ventricular ejection, which are volume-dependent parameters of myocardial performance, the mean rate of circumferential shortening appears to be a reasonable index of left ventricular contractility, which in steady-state conditions is independent of left ventricular end-diastolic volume and mean ventricular wall force of ejection. In this study, changes in mean rate of circumferential shortening associated with changes of heart rate were small and variable.


Epinephrine: selective inhibition of the acute insulin response to glucose. An epinephrine infusion of 6 mug/min decreased the rapid insulin response to a 5 g glucose pulse by 96% (P < 0.001) compared with the preinfusion control. In contrast when an identical epinephrine infusion was superimposed on a prolonged glucose infusion, elevated steady-state insulin levels did not decrease, but increased from 26.9 +/-6 (mean +/-SD, muU/ml) to 56.8 +/-15 muU/ml (P < 0.05) in parallel with the epinephrine-induced hyperglycemia. Thus epinephrine inhibition of insulin secretion was observed during acute but not chronic glucose stimulation. To evaluate further the insulin responses during a prolonged glucose infusion, a 5 g glucose pulse was given before and 60 min later during a concomitant epinephrine infusion. Although the acute insulin response to the first glucose pulse was observed during the elevated steady-state glucose and insulin levels associated with the glucose infusion, epinephrine again inhibited the acute insulin response to the subsequent 5 g glucose pulse by 91% (P < 0.01). Thus epinephrine appears to inhibit selectively the rapid insulin response to glucose but not to influence insulin output stimulated by prolonged hyperglycemia. These observations provide further evidence for a model of insulin secretion which includes a small storage pool available for immediate release to a glucose challenge and a more slowly responding pool regulating insulin secretion in the basal and steady state.


Functional compensation in partially denervated muscles. In patients with various types of chronic motor denervation, the numbers of surviving motor units have been compared with the twitch tensions developed by the same muscle (extensor digitorum brevis). It was found that functional compensation in partially denervated muscles was often marked; in most patients abnormally small twitches occurred only when fewer than 10% of motor axons remained. The factors responsible for this compensation are considered. The twitch speeds of partially denervated muscles differed markedly, even among patients with the same disorder; there was evidence to suggest that the twitches of some motor units might become slower than those found in normal muscles.


An electrophysiological study of Duchenne dystrophy. An electrophysiological study has been made of extensor digitorum brevis muscles in 19 boys with Duchenne dystrophy. The isometric twitches of the dystrophic muscles developed less tension and were usually slower than those in controls. Impulse conduction velocity appeared to be reduced in distal regions of nerve axons but was normal proximally. The most interesting finding was a reduction in the number of functioning units in all but one of the patients. The sizes of the surviving units suggested that the results could be explained more easily in terms of a neurogenic process than a primary myopathic one.


Amine uptake and storage mechanisms in the corpus striatum of rat and rabbit.


Effect of lateral hypothalamic lesions on uptake of norepinephrine by brain homogenates.


Adrenergic responses in the human eye.

Adrenergic responses in the human eye.


Tocopherols in foods and fats.

Tocopherols in foods and fats.


Intracranial midline echo shift and width.


Echocardiography of the mitral valve.

Echocardiography of the mitral valve.


Management of the acutely injured hand.

Management of the acutely injured hand.


Urological care for the child with myelodysplasia.


Dr. Robert N. Crews at century mark.

Dr. Robert N. Crews at century mark.


Delayed reconstruction for postradiation defect of the middle ear. Case report.


Treatment of end-stage kidney disease in Massachusetts.


Tumor angiogenesis: therapeutic implications.


The lymphatics of the larynx.

The lymphatics of the larynx.


Surgery of limited lesions of the larynx (past and present).


Histopathologic problems and frozen section diagnosis in diseases of the larynx.


Elective neck dissection in carcinoma of the larynx.


Radiotherapy of the larynx.

Radiotherapy of the larynx.


Surgery for extensive malignancies of the larynx.


Voice diagnostics and therapy.

Voice diagnostics and therapy.


Isaac Kaminer, M.D. 1834-1901 physician, poet, and satirist.


The history of groin hernia.

The history of groin hernia.


Flat foot. II.

Flat foot. II.


Jacobus Albertus van der Merwe Louw, M.B., Ch.B. (Cape Town).


Focal fungal infections treated by immunological therapy with emphasis on vaginal moniliasis.


Report of two cases. Primary melanoma arising in site of previous melanoma.


Streptomycin plus PAS plus pyrazinamide in the retreatment of pulmonary tuberxulisis in East Africa.


Computer-aided procedures in a multi-centre co-operative controlled clinical trial in pulmonary tuberculosis.


Bacteriological and radiographic features of lung infection by opportunist mycobacteris: a review.


Frank Alfred Kay: physician-psychiatrist.


Transmutation into protector of consumer health services.


Flammable fabrics and the burn problem: a status report.


A selected public health bibliography with annotations.


Public health: then and now. Compulsory health insurance in California, 1934-1935.


Peptic ulcer of the pyloric region.

Peptic ulcer of the pyloric region.


Angiography of the greater omentum.

Angiography of the greater omentum.


Lester G. Ericksen, M.D. 1899-1971.

Lester G. Ericksen, M.D. 1899-1971.


Effect of cromolyn sodium on exercise-induced airway obstruction in asthmatic children.


Preparation of  125 I labelled alum-precipitated ragweed antigens and a comparison of urinary excretion rates in the rat.


Gordon-Taylor, breast cancer and the Middlesex Hospital.


Grey Turner and the evolution of oesophageal surgery.


Kidney transplantation.

Kidney transplantation.


Toward a biology of affective disorders. Genetic contributions.


The validity of the Eysenck personality inventory studies by a technique of peer-rating item by item, and by sociometric comparisons.


Thiopropazate hydrochloride in persistent dyskinesia. Thiopropazate (Dartalan) was found to be significantly more effective than a placebo in relieving dyskinesia in 23 patients with functional psychosis and persistent dyskinesia associated with prolonged phenothiazine therapy. Each patient whose dyskinesia had persisted unchanged for at least one month after phenothiazine withdrawal received thiopropazate by mouth for three weeks and the placebo for a similar period. Patients were evaluated before the trial, at three weeks, and at six weeks.The drug also improved psychotic behaviour. Possible side effects, which were generally mild, were noted in eight patients, of whom six had Parkinsonism and four drowsiness. None had side effects while on the placebo.The findings indicate that thiopropazate is of value in persistent dyskinesia associated with prolonged phenothiazine intake-a condition hitherto unresponsive to other treatment. Further research is required to determine the long-term effectiveness of the drug.


Baclofen in the treatment of spasticity. Baclofendagger (Lioresal), a derivative of gamma-aminobutyric acid, was introduced in 1966 as a possible treatment for spasticity due to corticospinal tract lesions. Preliminary studies suggested that it may be more effective than other spasmolytic agents currently available, and a double-blind controlled trial in a group of 23 patients against placebo has shown it to be significantly more effective.


Persistent phenothiazine dyskinesia treated with tetrabenazine. Six patients with persistent phenothiazine dyskinesia were treated in a double-blind controlled trial with tetrabenazine 100 mg in divided dosage. In three patients the abnormal movements were abolished and in two others there was some improvement, but this was no greater than that achieved with the diazepam control. Tetrabenazine may be useful in the treatment of some patients with persistent phenothiazine dyskinesia.


Somatic cell genetics.

Somatic cell genetics.


Delayed hypersensitivity-induced regression of human neoplasms.


Transmission of cancer in man. Tentative guidelines referring to the possible effects of inoculation of homologous cancer extracts in man.


Hydroxyurea-induced inhibition of DNA puff development in the salivary gland chromosomes of Bradysia hygida.


Mechanisms of pentobarbital actions on prolactin release.


Antibiotic sensitivities of common bacterial pathogens isolated in Addis Ababa; a preliminary report.


Effects of hypothalamic stimulation on prolactin secretion in steroid treated rats.


A new approach to the treatment of some chronic respiratory diseases.


Problem solving and behavior modification.


Obituary--John P. Glynn.

Obituary--John P. Glynn.


Jan-Friedrich Tönnies.

Jan-Friedrich Tönnies.


Stages in the formation and keratinization of the cortex of the wool fiber.


A further contribution on the ultrastructure of calcifying cartilage.


Ultrastructure of complex nuclear bodies produced experimentally in hamster peritoneal macrophages.


Etiology of pyelonephritis: further evidence associating the production of experimental pyelonephritis with hemolysis in Escherichia coli.


Plants used against cancer. A survey.

Plants used against cancer. A survey.


Iatrogenic pneumaturia.

Iatrogenic pneumaturia.


Spontaneous bacterial peritonitis in cirrhosis: variations on a theme.


Selective IgA deficiency: presentation of 30 cases and a review of the literature.


Effect of UV irradiation and  32 P decay on thymineless death in E. coli 15.


Mutagenicity of N-nitrosomorpholine in the host-mediated assay.


Catheter closure of patent ductus arteriosus. 62 cases treated without thoracotomy.


Radiologic aspects of cardiac transplantation.


Radiologic findings resulting from thoracic aortic surgery--congenital and acquired diseases.


Radiologic evaluation of traumatic heart disease.


Radiologic diagnosis of late complications associated with cardiac valve surgery in acquired heart disease.


Catecholamines in chronic respiratory insufficiency.


Leonard Bostock, L.R.C.P. & S. (Edin.), L.R.P.S. (Glasg.), D.T.M. & H.


Ampicillin hypersensitivity in lymphatic leukaemia.


The present role of surgery in congenital heart disease.


Fluorescence studies of biopolymer structure.


Functional aspects of the cholestatic response induced by  -naphthylisothiocyanate in mice and rats.


Pasteur's fixed virus of rabies.

Pasteur's fixed virus of rabies.


Are biogenic amines involved in the mechanism of action of the tetrahydrocannabinols?


Conjunctival swabbing for the isolation of TRIC agent (Chlamydia).


Factors influencing the dental management of renal transplant and dialysis patients.


Parkinsonism--a so-called new disease.

Parkinsonism--a so-called new disease.


Oxygen therapy for spontaneous pneumothorax. The rate of absorption of gas from a pneumothorax was studied in 12 patients breathing air and in 10 patients breathing air and a high concentration of oxygen alternately. The mean rate of absorption while breathing air was the same in both groups of patients. In the second group the rate consistently increased during periods of oxygen therapy and decreased again on breathing air. The mean rate of absorption increased fourfold during periods of oxygen therapy. This increase was most pronounced in patients with a large pneumothorax.


The halothane dilemma: a case for the defence.


Bone crises and chronic osteomyelitis in Gaucher's disease.


Desmoplastic fibroma of the radius. Report of a case with segmental resection.


Fractures in myelodysplasia.

Fractures in myelodysplasia.


Posterior transposition of tibialis anterior through the interosseous membrane.


DDT and juvenile jaundice.

DDT and juvenile jaundice.


Drug interference in clinical biochemistry.


The bacteria-like Chlamydiae of ornithosis and the diseases they cause.


New aspects of the structure, function, and synthesis of hemoglobins.


I. Alteration in the properties of human hemoglobin A by variation in structure: a correlation of structure and function.


II. Binding of organic phosphates to hemoglobin.


3. Hemoglobin heterogeneity and structural genes.


Blood bank inventories.

Blood bank inventories.


Chromosome structure and function in man. I. Pachytene mapping in the male, improved methods and general discussion of initial results.


IV. The chemical heterogeneity of the   chain from human fetal hemoglobin.


Light and electron microscopic observations on rat thyroid after administration of some dithiocarbamates.


Beating of myocardial cells in monolayer culture. Inhibition by phospholipase C and restoration by lysophosphatides.


Cilia on cultured ependymal cells examined by scanning electron microscopy.


Symbiosis of bacteria with eggs of Dentalium at the vegetal pole.


Allergy and migraine: a clinical study.

Allergy and migraine: a clinical study.


Clinical and experimental evaluation of a new ergot-derivative (ergostine) in the treatment of migraine.


B.C.G. test in tuberculosis.

B.C.G. test in tuberculosis.


Computer simulation of a living cell. II.


Symptom diagnosis: the use of two different mathematical models.


Sporotrichosis in a cat.

Sporotrichosis in a cat.


Environmental--thermoregulatory interactions and neonatal piglet survival.


The optic nerve of the mallard duck: fiber-diameter frequency distribution and physiological properties.


The telencephalon and hypothalamus of the bluegill (Lepomis macrochirus): evoked feeding, aggressive and reproductive behavior with representative frontal sections.


Colonic secretion of water and electrolytes induced by bile acids: perfusion studies in man. Each of the three major bile acids of man was tested for its influence on electrolyte and water absorption in the human colon. Transport from isotonic solutions, with or without added bile acids, was compared in 35 studies on 20 healthy volunteers by colonic perfusions under steady-state conditions. Electrolytes and water were always absorbed from control solutions, but dihydroxy bile acid solutions induced continuous secretion or inhibition of sodium, potassium, and water absorption, which was reversible. Deoxycholic acid caused consistent secretion at 3 mm concentrations, whereas chenodeoxycholic acid did not induce secretion until the concentration was 5 mm. The trihydroxy bile acid (cholic acid) produced no significant change in absorption at 10 mm. Inhibition of absorption was also induced by mixtures of the glycine or taurine conjugated bile acids. Secretion of sodium and chloride, induced by bile acid perfusion, was linearly correlated with secretion of water; potassium secretion was relatively constant regardless of the volume of secretion. These results establish a striking influence of bile acids on colonic absorptive activity, provide an explanation in part for the diarrhea that frequently accompanies ileal disease or resection, and imply that diarrhea should occur in other disease states that produce elevated concentrations of dihydroxy bile acids in the colonic lumen.


Kansas in the fight against tuberculosis.


A simple primate headholder.

A simple primate headholder.


Clinical aspects of the Loney draw-a-car test: enuresis and encopresis.


Testing of blood donors for syphilis.

Testing of blood donors for syphilis.


The economis of computerized clinical practice.


Corneal grafting.

Corneal grafting.


The renal response to streptococcal infection.


The effect of early bone grafting on the growth of upper jaw in cleft lip and palate children. A computer evaluation.


Local origin of fibroblasts deduced from sarcomas induced in chimaeras by implants of pliable disks.


Induction of zona reaction in golden hamster eggs by cortical granule material.


Stereochemical and vibrational theories of odour.


Postnatal malnutrition and brain cholinesterase in rats.


Evidence for inhibition by brain serotonin of mouse killing behaviour in rats.


Retraction and expansion of the dendritic tree of motor neurones of adult rats induced in vivo.


Fluid balance in the Sahara.

Fluid balance in the Sahara.


Standing with a bent knee.

Standing with a bent knee.


Mechanism of absorption of phosphate from soil by Endogone mycorrhizas.


Function and nomenclature for two mammalian copper proteins.


Results of dietary treatment in phenylketonuria.


Structure and function of the jejunum in kwashiorkor.


Enhancement of liver glucose-6-phosphate dehydrogenase by kietary carbohydrate and insulin.


Metabolism of fatty acids of the palmitoleate series in the fat-deficient rat.


Changing patterns of mortality in burns.


A late report on an early operation for "baggy eyelids".


The classic reprint. On staphyloraphy.

The classic reprint. On staphyloraphy.


The classic reprint. Observations on cleft palate and on staphyloraphy.


The classic reprint. Cleft palate--velosynthesis.


Total reconstruction of upper and lower eyelids after loss of lids and globe. Case report.


The healing of wounds after delayed primary closure. An experimental study.


Contraceptive technology: current and prospective methods.


Experimental erysipelothrix arthritis. I. Observations on specific-pathogen-free and gnotobiotic pigs following systemic administration of live E. rhus iopathiae or intra-articular injection of non-living E. rhusiopathiae cells or cell wall fragments.


Studies on the immunotherapy of tetanus. I. Purification and characterization of tetanus toxin.


Clinical applications of brain blood flow studies.


Current status of brain scanning.

Current status of brain scanning.


Radioactive xenon in the evaluation of regional lung function.


The physiologic factors affecting regional ventilation and perfusion.


Pinch grafts for cutaneous ulcers.

Pinch grafts for cutaneous ulcers.


Oral contraceptives and untoward effects coincident with their use: a review.


Spina bifida survey.

Spina bifida survey.


Removal of white blood cells from gametocyte-, schizont-, trophozoite- and ring stages of Plasmodium falciparum.


Two fatal cases of P. falciparum malaria from tropical Africa.


The laboratory diagnosis of cholera with special reference to El Tor vibrio.


Device for marking microscope slides for use in indirect immunofluorescence work.


Studies of splenomegaly in rodent malaria. II. The course of splenomegaly, IgM, IgG levels and IgG immunofluorescent antibody titre in mice after infection with Plasmodium berghei yoelii and-or Plasmodium chabaudi.


Drug potentiation against pre-erythrocytic stages of Plasmodium falciparum.


Comprehensive health operating and information systems.


Fiftieth anniversary of the Registry of Ophthalmic Pathology.


Potential influence of computer-based systems on health services.


A computer-compatible solution to the medical record problem.


Induction of tolerance to skin homografts with a methylhydrazine derivative. Synergism with antilymphocytic serum and morphologic responses of the lymphoid system.


Notes and documents concerning the discovery of LSD.


Scientific autographs. 3. L. Pasteur: rabies conquered.


Intestinal mucous membranes: the role of the interfacial phenomena.


Isolation of L-forms in recurrent urinary tract infections.


Clinical use of prostaglandins as oxytocin substances.


John Aylward Lewis.

John Aylward Lewis.


An immunological study of an enzyme made by phage containing 5-iodo-2'-deoxyuridine-substitted deoxyribonucleic acid.


Prostaglandins in family planning strategy.


Recent advances in pain relief in childbirth. II. Regional anaesthesia.


The foetal and maternal pharmacology of some of the drugs used for the relief of pain in labour.


Resuscitation of the newborn.

Resuscitation of the newborn.


Anaesthesia for obstetric emergencies.

Anaesthesia for obstetric emergencies.


Providing supportive services to the elderly.


The use of computers in surgical practice.


Computers with regard to surgical records and notes.


Diagnosis and diagnostic patterns.

Diagnosis and diagnostic patterns.


Epidemiology of cancer of the oesophagus and stomach.


Idiopathic Parkinsonism treated with an extracerebral decarboxylase inhibitor in combination with levodopa. The clinical actions of levodopa in Parkinsonism, given with and without an extracerebral decarboxylase in hibitor, L-alpha-methyldopahydrazine, were compared. Twenty-one patients were investigated in a "double-blind cross-over" study, administering levodopa in maximum tolerated dosage. L-Alpha-methyldopahydrazine failed to augment the overall therapeutic actions of levodopa but it consistently alleviated nausea. It is concluded that L-alpha-methyldopahydrazine will prove useful in the management of some Parkinsonian patients who have difficulty in taking levodopa alone.


Trimethoprim and sulphamethoxazole in typhoid fever in children. One hundred and three children with proved typhoid fever were treated with trimethoprim-sulphamethoxazole, and the results compared with those of a further 40 children treated with chloramphenicol. The bacteriological response to trimethoprim-sulphamethoxazole was unsatisfactory. From this study it seems that at present chloramphenicol is still the treatment of choice for typhoid fever. In view of the haematological changes occurring during therapy with trimethoprim-sulphamethoxazole caution is necessary and monitoring of the blood picture advisable, even at the recommended dose.


The virgin queen (Elizabeth I).

The virgin queen (Elizabeth I).


Prophylaxis of wound infection.

Prophylaxis of wound infection.


S-adenosylmethionine in human blood.

S-adenosylmethionine in human blood.


A comparison of two colorimetric procedures for the assay of 5'-nucleotidase.


If Sherlock Holmes had been a gerodontist.


The effect of various sugars on the formation and chemical composition of dental plaque.


Repair of large incisional hernias.

Repair of large incisional hernias.


Sectional rhytidectomy.

Sectional rhytidectomy.


H.M. Klenda, former ADA President, dies.


Col. E. Smith to head Army Dental Corps.


Cold therapy revisited.

Cold therapy revisited.


Research training for careers in academic dentistry.


Anton van Leeuwenhoek and the tooth-worm.


Immunocompetent cells of the chicken. I. Specific surface antigenic markers on bursa and thymus cells. Specific antisera to chicken thymus and to bursa of Fabricius were obtained in rabbits. After appropriate absorption and dilution all four anti-thymus sera, in the presence of guinea pig C', killed >90% of thymus and <==12% of bursa cells. They were cytotoxic for approximately 50% of spleen cells and did not affect antibody-forming cells. The surface antigen detected by these antisera was named chicken T-lymphocyte antigen (CTLA). Two of four anti-bursa sera, under similar conditions, killed >90% of bursa cells and <==10% of thymus cells. These antisera were cytotoxc for a large percentage of antibody-forming cells and killed approximately 30% of spleen cells The other two anti-bursa sera were somewhat less potent but showed similar specificity. The surface antigen detected by these antisera was named chicken bursa-derived lymphocyte antigen (CBuLA). Rabbit antisera to chicken immunoglobulin were cytotoxic for bursa but not for thymus cells and killed a similar percentage of spleen cells as did anti-bursa sera. They were also cytotoxic for antibody-forming cells.


Nonantigenicity and immunologic tolerance: the role of the carrier in the induction of tolerance to the hapten. Treatment of adult mice with dinitrophenyl (DNP) bound to isogeneic serum resulted in a specific inability to respond to DNP after challenge with DNP-keyhole limpet hemocyanin (KLH) in complete Freund's adjuvant. The unresponsiveness to the hapten had all the characteristics of immunologic tolerance: it had a definite induction time; it was transient but could be maintained by additional injections of the tolerogen; it was antigen specific and dose dependent. In addition, the induction of tolerance to DNP is dependent on the nature of the carrier. Two main conclusions can be drawn from these data: DNP conjugates of three homopolymers of lysine were found to be nonimmunogenic in mice, yet tolerogenic. Thus, antigenicity is not necessary to induce tolerance. Among the various carriers tested, isogeneic 7S immunoglobulin (IgG) was found to be the most effective to induce and maintain tolerance to the hapten. This suggests that IgG may have a function other than its usual role as an immunoglobulin.


The immune systems of mice reared in clean and in dirty conventional laboratory farms. II. Primary antibody-forming activity of young and old mice with long life-spans.


Macrophage-melanoma cell heterokaryons. IV. Unmasking the macrophage-specific membrane receptor. Mouse peritoneal macrophages possess a specific plasma membrane receptor for antibody-coated particles. Sheep red cells coated with rabbit 7S antibody attach readily to the macrophage surface and are subsequently interiorized. The fusion of macrophage with nonphagocytic mouse melanoma cells produces heterokaryons in which the macrophage receptor is drastically altered. The receptor is present shortly after fusion and heterokaryons are actively phagocytic. The ability to bind and ingest red cells is, however, progressively lost over the next 12-24 hr and does not reappear thereafter. Exposure of heterokaryons to trypsin (1-100 microg/ml for 30 min at 37 degrees C) results in the reappearance of initial receptor activity and the unmasking of the surface receptor. This property is again lost upon subsequent cultivation. The masking process takes place when cells are cultivated in the absence of IgG so that the adsorption of antibody from the medium is not responsible for this phenomenon. Inhibition of heterokaryon protein synthesis preserves phagocytic activity in a reversible fashion and prevents the masking of macrophage receptors. Inhibition of melanoma RNA synthesis before fusion is also able to block subsequent masking, but is ineffective if delayed until after fusion. Ultraviolet irradiation of the melanoma cell before fusion prevents subsequent masking, whereas similar treatment of the macrophage has no effect. Cells differ markedly in their ability to mask the macrophage phagocytic receptor after fusion. Ehrlich ascites tumor cells mask the receptor rapidly, primary chick fibroblasts minimally, and embryonic chick erythrocytes not at all.


Current publications in gerontology and geriatrics.


The immune systems of mice reared in clean and in dirty conventional laboratory farms. 3. Ability of old mice to be sensitized to undergo a secondary antibody response.


Observations on tissue grafts established in rabbit ear chambers. A combined light and electron microscopic study. An in vivo microscopic and ultrastructural study of tissues transferred to the transparent rabbit ear chamber is presented. Fragments of liver, kidney, thyroid, and myometrium were successfully auto- or allografted into the chamber from donors of all ages and allowed continuous in vivo observation of parenchymal structure and function, as well as of the graft vasculature which plays such an active role during rejection. Circulation was quickly reestablished by anastomosis of graft vessels to those of the ear chamber membrane and only minor reversible parenchymal changes occurred. Both vessels and parenchyma retained the characteristics of the organs of origin on both light and electron microscopy and were observed functioning in vivo for periods up to 1 yr in the case of autografts, and until rejection occurred in allografts. In the latter case, rejection did not occur in tissues obtained from adult and neonatal donors for nearly 3 months, while tissues of fetal origin were generally rejected much earlier. The kidney grafts provide a unique opportunity for a close comparative study of mammalian fetal and adult glomerular blood flow under varying rates of perfusion, and the tubular epithelium could be observed regenerating after initial acute tubular necrosis. Renal tubules from fetal, neonatal, and adult donors were all capable of metaplastic change to form a highly ciliated epithelium. Grafts of these tissues will allow the fine detail of the processes of rejection to be studied continuously in vivo.


Isolation of rabies virus from small wild rodents.


Specificity of cholera diagnostic bacteriophages on identification of vibrios isolated from open water reservoirs.


Utilization of differentiation of antibodies to Shigella organisms according to the immunoglobulin class in the serological diagnostics of dysentery and in study of its epidemiology.


Further study of fimbrial antigens in Enterobacteriaceae. 3. Distribution of the fimbrial phase in the genus Shigella.


Experimental inhalation tularaemia: statistical distribution of infectious particles deposited in the lung tissue in guinea pigs.


Antistreptolysin Q--immunological surveys in epidemiological analysis.


Study of transmissible polyresistance in routine laboratory practice.


Academician A.A. Smorodintsev (on the occasion of his 70th birthday).


The 'law of ancestral heredity' and the Mendelian-ancestrian controversy in England, 1889-1906.


Is there an equivalent in the house?

Is there an equivalent in the house?


Computer analysis of labor progression. 3. Pattern variations by parity.


Inhibition of gastric secretion by atropine in conscious rats. 1. The effect of atropine on acid and pepsin secretion, stimulated by feeding, vagus excitation or infusions of gastrin, histamine and methacholine, was studied in conscious rats provided with a Pavlov or a Heidenhain pouch.2. The acid and pepsin secretion in response to food was substantially suppressed by atropine in the Pavlov pouch.3. The strong stimulatory effect of 2-deoxy-D-glucose on acid and pepsin secretion in the Pavlov pouch was almost abolished by atropine.4. A plateau secretion of acid obtained by infusion of submaximal doses of hog gastrin II, was significantly inhibited by atropine in the Pavlov and the Heidenhain pouch.5. Methacholine-induced acid and pepsin secretion in the denervated Heidenhain pouch was nearly completely inhibited by atropine.6. A plateau secretion of acid evoked by infusing histamine in a near maximal dosage was inhibited by atropine to the same extent as the gastrin-induced secretion in the Pavlov pouch.7. The mode of action of atropine as a gastric secretory inhibitor is discussed.


In appreciation of Frank E. Whitacre.

In appreciation of Frank E. Whitacre.


Directory of otolaryngologic societies.

Directory of otolaryngologic societies.


A study of Mycoplasma pneumoniae infections in families.


Multiple virus isolations in a group of children hospitalized with acute respiratory illness.


Single dose immunization against tetanus. Promising results in human trials.


Effects on Bay Va 1470 on cardiovascular parameters in ponies.


Evaluation of Vetalar (ketamine HCl). A unique feline anesthetic.


Diagnostic bacteriology: identification of aerobic pathogens.


Cellular sites of immunoglobulins. II. The relative proportions of mucosal cells containing IgG, IgA, and IgM, and light polypeptide chains of kappa and lambda immunoglobulin in human appendices.


Cellular sites of immunoglobulins. 3. Localization of IgG-, IgA-, IgM- and their kappa and lambda light chain-containing cells in human palatine tonsils.


Pneumatosis cystoides intestinalis--bullous emphysema of the intestine. A review of the literature.


The question of bile regurgitation as a cause of gastric ulcer.


Relative importance of cellular and humoral immunity in human renal transplantation.


Investigation on the effect on skin healing of a cream containing dexamethasone, chlorhexidine hydrochloride and nandrolone decanoate.


Subtotal parathyroidectomy in chronic renal failure: a seven-year experience in a dialysis and transplant program.


Lorimer Fenton Dods.

Lorimer Fenton Dods.


Family planning education.

Family planning education.


Growth and development.

Growth and development.


Adolescent sexual behavior: current status and anticipated trends for the future.


Relation of nutrition to pregnancy in adolescence.


Fertility regulation for teen-agers.

Fertility regulation for teen-agers.


Adolescent out-of-wedlock pregnancy: an overview.


Venereal disease in teen-agers.

Venereal disease in teen-agers.


Trends and developments in social services for teen-age unmarried mothers.


The teen-age unwed father.

The teen-age unwed father.


Comprehensive community programs for the pregnant teen-ager.


Changing concepts regarding exchange transfusions and neonatal jaundice.


The endodontic culture technique: an impractical and unnecessary procedure.


Tooth contacts in denture occlusion--centric occlusion only.


Medicaid.

Medicaid.


Prepayment of medical services for the aged: an analysis.


A comparative study of conventional premedication (pethidine, promethazine, and atropine) and neuroleptanalgesia (droperidol and phenoperidine) for peroral endoscopy. A double blind comparison of conventional premedication (pethidine, promethazine, and atropine) and neuroleptanalgesia (droperidol and phenoperidine) failed to demonstrate any difference in either the comfort of the patient or ease of instrumentation in 70 upper gastrointestinal tract endoscopies. Further trials are needed before conventional premedication is abandoned.


Factitial proctitis: a restudy at the University of Minnesota.


Acute haemorrhagic gastritis: modern concepts based on pathogenesis.


The place of surgery in Crohn's disease.


"Card injuries of the hand" Associated with multiple broken pins.


Burns of the dorsum of the hand.

Burns of the dorsum of the hand.


Deep burns--burn contractures.

Deep burns--burn contractures.


Lactate, lactate dehydrogenase and protocollagen proline hydroxylase in rat skin autograft.


Regulation of food intake in ruminants. 8. Caloric density of diets for young growing lambs.


Biosynthesis of lactose.

Biosynthesis of lactose.


Molecular aspects of polyene- and sterol-dependent pore formation in thin lipid membranes. Amphotericin B modifies the permeability properties of thin lipid membranes formed from solutions containing sheep red cell phospholipids and cholesterol. At 10(-6)M amphotericin B, the DC membrane resistance fell from approximately 10(8) to approximately 10(2) ohm-cm(2), and the membranes became Cl(-)-, rather than Na(+)-selective; the permeability coefficients for hydrophilic nonelectrolytes increased in inverse relationship to solute size, and the rate of water flow during osmosis increased 30-fold. These changes may be rationalized by assuming that the interaction of amphotericin B with membrane-bound sterol resulted in the formation of aqueous pores. N-acetylamphotericin B and the methyl ester of N-acetylamphotericin B, but not the smaller ring compounds, filipin, rimocidin, and PA-166, produced comparable permeability changes in identical membranes, and amphotericin B and its derivatives produced similar changes in the properties of membranes formed from phospholipid-free sterol solutions. However, amphotericin B did not affect ionic selectivity or water and nonelectrolyte permeability in membranes formed from solutions containing phospholipids and no added cholesterol, or when cholesterol was replaced by either cholesterol palmitate, dihydrotachysterol, epicholesterol, or Delta5-cholesten-3-one. Phospholipid-free sterol membranes exposed to amphotericin B or its derivatives were anion-selective, but the degree of Cl(-) selectivity varied among the compounds, and with the aqueous pH. The data are discussed with regard to, first, the nature of the polyene-sterol interactions which result in pore formation, and second, the functional groups on amphotericin B responsible for membrane anion selectivity.


Early replicative form DNA of bacteriophage S13. I. Sucrose gradient analysis of replicative form made by gene IV mutants.


Experimental approaches to the stem cell problem in postnatal life.


Intra- and extracellular compartmentalization of the surface-active fraction in dog lung. Adult mongrel dogs were killed at various times after injection of (3)H-labeled palmitate. The lungs were removed and subjected to an extensive saline lavage. The surface-active fraction was isolated from the lavage and from homogenized residual lung by a procedure based upon differential centrifugation in sucrose solutions. The material isolated from the lavage was designated extracellular surfactant; material from the residual lung was designated intracellular surfactant. Both had similar chemical composition and surface activity. The results of the isotopic labeling studies demonstrate that the two fractions have distinctly different specific activity curves. Label was incorporated into the intracellular surfactant rapidly and reached a peak at 1 hr. No radioactivity was found in the extracellular surfactant for the first 15 min, and the specific activity increased much more slowly than in the intracellular surfactant. These results demonstrate at least two anatomically distinct metabolic "pools" of pulmonary surfactant in the lung. While our data are not conclusive, one possible interpretation is that the biosynthesis of pulmonary surfactant takes place intracellularly with a subsequent secretion onto the alveolar surface.


Polypeptide chain termination: an active process.


Site of synthesis of viral ribonucleic acid and phage assembly in MS2-infected Escherichia coli.


Physiology of bacteriophage lambda in the restrictive host.


Isolation of temperature-sensitive Lac repressors.


Studies on polynucleotides. LXXXV. Partial purification of an amber supressor tRNA and studies on in vitro suppression.


Fluorescent antibody localization of erythropoietin in the glomerular tuft of the kidney.


Studies on Escherichia coli sex factors. 3. Covalently closed F'Lac DNA molecules.


Titration of the gene sites on DNA by DNA-RNA hybridization. I. Problem of measurement.


Titration of the gene sites on DNA by DNA-RNA hybridization. II. The Escherichia coli chromosome.


Analysis of amino acid replacements resulting from frameshift and missense mutations in the tryptophan synthetase A gene of Escherichia coli.


Mutants of bacteriophage lambda defective in vegetative genetic recombination.


Synthesis of ribosomal proteins and formation of ribosomes in Escherichia coli.


Control of RNA synthesis in Escherichia coli. I. Amino acid dependence of the synthesis of the substrates of RNA polymerase.


Endonuclease-I-deficient and ribonuclease I-deficient Escherichia coli mutants.


On the assay, isolation and characterization of the lac repressor.


DNA binding of the lac repressor.

DNA binding of the lac repressor.


The sequence of 5 s ribosomal ribonucleic acid.


Ribonucleic acid codons and protein synthesis. 13. RNA codon recognition by deacylated tRNA and aminoacyl-tRNA.


Intracellular development of bacteriophage phiR. I. Host modification of the replicative process.


Chain growth rate of messenger RNA in Escherichia coli infected with bacteriophage T4.


External suppression of a frameshift mutant in salmonella.


Structure and function of Escherichia coli ribosomes. I. Partial fractionation of the functionally active ribosomal proteins and reconstitution of artificial subribosomal particles.


Structure and function of Escherichia coli ribosomes. II. Translational fidelity and efficiency in protein synthesis of a protein-deficient subribosomal particle.


Structure and function of Escherichia coli ribosomes. 3. Stoichiometry and rate of the reconstitution of ribosomes from subribosomal particles and split proteins.


Synthesis and maturation of phage P22 DNA. I. Identification of intermediates.


Synthesis and maturation of phage P22 DNA. II. Properties of temperature-sensitive phage mutants defective in DNA metabolism.


Crystals of tropomyosin and native tropomyosin.


Permanent association of 5 s RNA molecules with 50 s ribosomal subunits in growing bacteria.


Identification of the transcribing DNA strand for the rII and endolysin genes of coliphage T4.


Studies on the nature of the block to polyspermy in rabbit ova.


Memoir. F. W. Rogers Brambell. (1901-1970).


Sociological aspects at a municipal hospital of cadaver renal transplantation.


The effect of diet on lipid metabolism.

The effect of diet on lipid metabolism.


Osteomalacia and anticonvulsant drugs.

Osteomalacia and anticonvulsant drugs.


Intestinal calcium transport mediated by vitamin D.


Metabolic effects of stravation in newborn pigs.


Hereditary defect in intestinal iron transport.


Methionine, vitamin E, and selenium toxicity.


Female sexuality and life situations. An etiologic psycho-socio-sexual profile of weight gain and nausea and vomiting in pregnancy.


Edgar Allen.

Edgar Allen.


Red blood cell and plasma volumes, total body water and sulfate space in obese-hyperglycemic mice and lean litter mates.


Formation of  14 C-octopamine from  14 C-tyramine in the mouse: a dose dependent relationship.


Graded sequential therapy in the menopause: a double-blind study.


Tc 99m  Na pertechnetate scanning of the abdomen with particular reference to small bowel pathology.


Necrotizing enterocolitis of infancy.

Necrotizing enterocolitis of infancy.


Lymphatic channel obstruction. Patterns of altered flow dynamics.


Methods of repair after surgery for cancer of the pharyngeal wall, postcricoid area, and cervical esophagus.


Hemodynamic assessment of poor risk and critically ill patients.


Aanastomotic leakage after excision of esophageal and high gastric carcinoma.


Humidification during positive-pressure ventilation of infants.


A comparison of four different methods for testing the production of a niacin by mycobacteria.


ACA committees--1970-71.

ACA committees--1970-71.


Doctor Truman C. Terrell 1890-1971.

Doctor Truman C. Terrell 1890-1971.


The Lombard reflex. Historical perspective.


Total laryngectomy and cancer of the larynx. A historical review.


Transcription of mitochondrial DNA in vitro from Neurospora crassa.


On the bactericidal action of levallorphan. Irreversible alterations of the plasmic membrane.


The protection by 70 S ribosomes of N-acyl-aminoacyl-tRNA against cleavage by peptidyl-tRNA hydrolase and its use to assay ribosomal association.


Karl-Günter Zimmer.

Karl-Günter Zimmer.


Electron affinic sensitization. II. Para-nitroacetophenone: a radiosensitizer for anoxic bacterial and mammalian cells.


Electrical coupling between fat cells in newt fat body and mouse brown fat. White fat from the newt, Triturus pyrrhogaster, fat body, and brown fat from the interscapular fat pad of newborn mice have been tested for the presence of low-resistance intercellular junctions. 42 pairs of amphibian fat cells and 15 pairs of mammalian brown fat cells were found to be "electrically coupled." In most of these cases intracellular deposition of a dye, Niagara Sky Blue: 6B, was used to supplement and confirm direct observations of impalements. Coupling was often difficult to find in both preparations, but the mechanical disturbance of the tissue during the preparative procedures may have uncoupled many cells. The fact that, in both types of fat, coupling was observed between cells separated by one or more other cells suggests that coupling may be more widespread in vivo. Electron microscopy (provided by Dr. J. -P. Revel and Mrs. K. Wolken) of the brown fat revealed frequent intercellular junctions resembling "gap junctions" but possibly lacking the substructure usually visible with colloidal lanthanum infiltration. The results are discussed in relation to current ideas about the exchange of regulatory molecules via low-resistance junctions and about the control of brown fat by hormones and nerves.


Daily and diurnal variations in the ratio of nitrogen to potassium in the urine of pigs for the estimation of the retained nitrogen.


Phagocytosis in vitamin D deficient rickets.


Lymphatic metastases of bone tumors.

Lymphatic metastases of bone tumors.


Evidence for RNA-DNA complexes in haemolytic plaque-forming cells containing ribonuclease resistant RNA.


Hypnosis--magic or quackery?

Hypnosis--magic or quackery?


Integration of medical research and health policies.


Gonadotropin-releasing hormone: one polypeptide regulates secretion of luteinizing and follicle-stimulating hormones. A polypeptide isolated from porcine hypothalami stimulates the release of both luteinizing hormone and follicle-stimulating hormone from the pituitaries of several species. This polypeptide has been structurally identified as (pyro)Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH(2) and synthesized. The natural and synthetic materials share biological properties. It appears that this peptide represents the hypothalamic hormone regulating the secretion of both luteinizing hormone and follicle-stimulating hormone.


Marihuana and memory: acquisition or retrieval? Two experiments were conducted to determine the means by which marihuana affects human memory. The results of these studies indicated that marihuana did not affect retrieval of information in memory when the method of free recall was used, but did affect recognition processes such that subjects were less able to discriminate between items that had been presented previously and items that had not appeared a short time before. With respect to initial learning, marihuana was shown to affect acquisition processes involved in the storage of information.


Burn problems illustrated by a case.

Burn problems illustrated by a case.


Ventricular aneurysmectomy.

Ventricular aneurysmectomy.


Current status of renal transplantation.


Ocular complications in cardiac surgery.


The surgery of renal transplantation.

The surgery of renal transplantation.


The surgical complications of renal transplantation.


Pathology of human renal allograft rejection.


Histocompatibility testing.

Histocompatibility testing.