Parathyroid surgery in the treatment of renal osteodystrophy and tertiary hyperparathyroidism.


Human kidney preservation for transplantation.


Musculoskeletal complications of renal transplantation.


The cytology of Hansenula. II. Germination of ascospores of Hansenula holstii.


Edwin J. Cohn memorial lecture. The fulfillment of his prophecy.


Hydrogensulphide producing variants of Escherichia coli.


Antibody responses in Mycoplasms pneumoniae infection in relation to serum immunoglobulins, especially IgM.


Determination of bile acid conversion potencies of intestinal bacteria by screening in vitro and subsequent establishment in germfree rats.


Extracellular water distribution and circulatory function of the penguin.


The activation of acetyl CoA carboxylase by tricarboxylic acids.


Genetic regulation of plasma cholinesterase in man.


Analysis of errors in electronic blood cell counting.


The mechanism of the erythrocyte sedimentation rate (ESR).


Formation, functions and regulatory importance of S-adenosyl-L-methionine.


Regulation of stem cell renewal and differentiation: possible significance in aging.


Failures of regulation mechanisms as causes of cellular aging.


Molecular approaches to the drug enhancement of deteriorated functioning in the aged.


Intravenous administration of essential amino acids to uremic patients on low protein diet.


The initiation of cell wall synthesis in parasynchronous cultures of Schizosaccharomyces pombe.


Amino acid and glucose fermentation by Treponema denticola.


Thiobacillus denitrificans as an obligate chemolithotroph. Isolation and growth studies.


The development of a hunger system in young chicks.


Assay of antibiotic activity in live-virus poultry vaccines with micro-techniques.


An exploration study of vocalization areas in the brain of the redwinged blackbird (Agelaius phoeniceus).


Ethology in the Soviet Union.

Ethology in the Soviet Union.


Preparation of Mycoplasma synoviae antigen for the tube agglutination test.


Ultrastructural alterations of the nucleus and the kinetoplast of Trypanosoma cruzi exposed to ethidium bromide.


Recruiting responses not dependent on orbito-frontal cortex.


Anti-tumour agents on antibody-forming cells in vitro.


Some therapeutic uses of Dacron vascular grafts.


Treatment of turkey hatching eggs for control of Arizona infection.


Characterization of a new infectious bronchitis virus isolate. I. Serological and pathogenicity studies of Clark 333.


The use of spectinomycin to control experimentally induced airsacculitis associated with Escherichia coli in chickens and turkeys.


Translocation of antibiotics in developing avian embryos.


Isolation of arizona from specimens grossly contaminated with competitive bacteria.


Anaerobic bacteria in postmortem blood cultures. Correlation with lesions of the gastrointestinal tract.


Reticulum cell sarcoma of the orbit.

Reticulum cell sarcoma of the orbit.


Harris Peyton Mosher.

Harris Peyton Mosher.


Enrichment serology compared with a direct-culture procedure for isolating salmonellae from rendered animal by-products.


What state governments can do.

What state governments can do.


Shortage of donor kidneys; extended organ preservation as one of the solutions.


Alternate-day prednisone therapy after renal transplantation.


Infection of the lung with Aspergillus fumigatus.


A new method for measuring gastric acid secretion in unanesthetized rats.


Functions of a pancreaticoduodenal allograft in man.


Growing role of early surgery in chronic pancreatitis: a practical clinical approach.


Acute pancreatitis.

Acute pancreatitis.


Recombination involving the ilv cluster of Salmonella typhimurium-Salmonella montevideo hybrids.


Histocompatibility differences in wild mice; further evidence for the existence of deme structure in natural populations of the house mouse.


Fungal (Candida albicans) growth after tetracycline therapy and its management.


Stapes surgery in deafness due to otosclerosis.


Transformation of 17 s to 16 s ribosomal RNA using ribonuclease II of Escherichia coli.


Origin and direction of bacteriophage T4 DNA replication. II. A gradient of marker frequencies in partially replicated T4 DNA as assayed by transformation.


Correlation between the peptidyl transferase activity of the 50 s ribosomal subunit and the ability of the subunit to interact with antibiotics.


Inactivation and reactivation of ribosomal subunits: amino acyl-transfer RNA binding activity of the 30 s subunit of Escherichia coli.


The role of sulfhydryl groups in the binding of RNA polymerase to synthetic polydeoxynucleotides.


Apparent transient false-positive FTA-ABS test following smallpox vaccination.


Medicine in the American Revolution. 3.

Medicine in the American Revolution. 3.


Medicine in ancient Egypt and its relevance today.


Renal transplantation: preparation of live and cadaver donors and techniques of nephrectomy.


Ureteral tailoring.

Ureteral tailoring.


A new "thumb" urethroplasty.

A new "thumb" urethroplasty.


Crossed ureteroureterostomy: variation of uses.


Epidermolysis bullosa.

Epidermolysis bullosa.


Therapeutic aspects of the doctor/patient relationship. Attempted measurement of some factors.


Tranquilizers in the treatment of the cardiac neurotic patient and the subsequent alteration of complaints.


Psychodynamic aspects in young patients suffering from peripheral vascular occlusions.


Radiation therapy. Historical perspectives.


The value of lymphography in tumours of the testes.


Toxoplasmosis as a cause of foetal death.


Mycoplasma pneumoniae pneumonia in Helsinki 1962-1970. Epidemic pattern and autoimmune manifestations.


Treatment of hookworm anemia.

Treatment of hookworm anemia.


Specific antibody response after transfusion of Australia antigen-positive blood in a patient with liver cirrhosis.


Oestrogen-binding and the hormone respinsiveness of tumours.


Who should pay for graduate surgical education: medicare and the Social Security Administration.


The impact of Quebec health services program on surgical training.


New technique of one-stage heterotopic pancreaticoduodenal autotransplantation in dogs.


Functional evaluation of the heart after storage under hypothermic coronary perfusion.


[Investigation of the effect of a new spasmolytic preparation (Spasmeks) on spastic colon].


The lateral cerebral ventricles of human foetuses with a crown-rump length of 26-178 MM.


Functional effectiveness of internal mammary artery grafts as estimated by myocardial  133 Xe washout.


The first anaesthesias in Hungary (1847-1849).


Aerospace Medical Association standing and special committees, 1971-72.


Comparison of the encephalographic, behavioral and subjective correlates of natural and drug-induced sleep at atypical hours.


Ingestive capacity of peritoneal macrophages from mice exposed to hypobaric hypoxia.


Feline viruses: pathogenesis of picornavirus infection in the cat.


Susceptibility of swine to mycobacterium paratuberculosis.


Transduction and mutation to glycine tolerance in vibrio fetus.


Influence of age and rations on fecal microflora of Hormel miniature swine.


The Arnold-Chiari malformations.

The Arnold-Chiari malformations.


Lengthening and shortening the palpebral fissure.


Deep fungal corneal abscess. Combined corticosteroid therapy.


Descartes' dream.

Descartes' dream.


A marking retraction suture for strabismus surgery.


Perspectives in biological psychiatry.

Perspectives in biological psychiatry.


Oral preparations of isoetharine compared with orciprenaline and choline theophyllinate.


Studies on the reactivation of bacteria photodamaged by psoralen.


Some genetic and physiological characteristics of urease-defective strains of Neurospora crassa.


Natural history of ventricular septal defect.


Patent ductus arteriosus in early infancy.


Pulmonary valvular stenosis.

Pulmonary valvular stenosis.


Congenital heart disease in adults.

Congenital heart disease in adults.


Surgical management of congenital heart disease; viewpoint of the surgeon.


Diagnosis of congenital heart disease: clues from the history and physical examination.


An historical sketch of medical education in Maine.


The reverse dermis graft--a choice not a flap.


Role modifications in tyrosine transfer RNA: a modified base affecting ribosome binding.


Operator mutants of the tryptophan operon in Escherichia coli.


RNA chain growth rates in Escherichia coli.


Orientation of transcription for the amber suppressor gene su 3 as determined by hybridization between tyrosine tRNA and the separated DNA strands of transducing coliphage phi80d su3.


Observations on the structure of RNA polymerase and its attachmnt to DNA.


a special purpose computer for the evaluation of left ventricular function.


A unitary account of the repression mechanism of arginine biosynthesis in Escherichia coli. I. The genetic evidence.


A unitary account of the repression mechanism of arginine biosynthesis in Escherichia coli. II. Application to the physiological evidence.


A ribosomal ambiguity mutation.

A ribosomal ambiguity mutation.


Identification of individual sex-factor DNA strands and their replication during conjugation in thermosensitive DNA mutants of Escherichia coli.


Initiation, elongation and inactivation of lac messenger RNA in Escherichia coli studied studied by measurement of its beta-galactosidase synthesizing capacity in vivo.


Polypeptide products of nonsense mutations. II. Minor fragments produced by nonsense mutations in the z gene of the lactose operon of Escherichia coli.


Health of slaves on southern plantations.


Bacteriuria: detection and screening techniques.


Plastic surgical treatment of facial skin cancer.


Plants used against cancer. A survey.

Plants used against cancer. A survey.


When your patient asks about plastic surgery.


Serology of histoplasmosis.

Serology of histoplasmosis.


Fluorescent antibody techniques for identification of Coccidioides immitis and Histoplasma capsulatum. (Ecological and epidemiological aspects).


Coccidioidomycosis in Mexico and Central America.


Mercury in water of the tidal Thames.

Mercury in water of the tidal Thames.


Histoplasmosis in Europe.

Histoplasmosis in Europe.


Mate-seeking by female Amblyomma maculatum (acarina: ixodidae) on a bovine.


Systemic action of an insect feeding deterrent.


Transplantation of radiation-induced canine myelomonocytic leukaemia.


Occurrence of histoplasmosis in Asia.

Occurrence of histoplasmosis in Asia.


Moleuclar pathology of human haemoglobin: stereochemical interpretation of abnormal oxygen affinities.


Persistent corpora lutea of mice in a cold environment.


Evaluation of compound probabilities in sequential choice.


Similarity measure for mixed attribute types.


Cosmic ray flashes in the eye.

Cosmic ray flashes in the eye.


Presence and function of disclinations in surface coats of unicellular organisms.


Bomb  14 C in the human population.

Bomb  14 C in the human population.


Daucic acid and its occurrence in plants.


Sir W. Le Gros Clark.

Sir W. Le Gros Clark.


Cytotoxic radiolysis products of irradiated alpha, beta-unsaturated carbonyl sugars as the carbohydrates.


Evidence for tRNA precursors in bacteria.


Effect of the mutations recB and polA on gamma-ray-induced DNA synthesis in a temperature sensitive dnaA mutant of Escherichia coli K12.


Structural changes in memebranes of synchronized cells demonstrated by freeze-cleavage.


Health care plans from the people who brought you Medicare. II.


Ovulation induced by synthetic luteinizing hormone-releasing hormone in the hamster. A synthetic decapeptide, corresponding to the chemical structure of luteinizing hormone-releasing hormone from porcine hypothalami, was tested for the induction of ovulation in golden hamsters that had previously been treated with phenobarbital to prevent spontaneous ovulation. Subcutaneous injection of 0.089 to 0.357 nanomole of this synthetic luteinizing hormone-releasing hormone stimulated release of luteinizing hormone and induced ovulation.


Ketamine anesthesia.

Ketamine anesthesia.


Marie François Xavier Bichat 1771-1802.

Marie François Xavier Bichat 1771-1802.


Some aspects of the Confederate Medical Service.


Laboratory diagnosis of toxoplasmosis.

Laboratory diagnosis of toxoplasmosis.


Medicare-B. Deceased beneficiary claims & recent Medicare contacts with physicians.


A fixation method for scanning electron microscopy of filamentous fungi.


Comparison of glomerular filtration rate and effective renal plasma flow determinations obtained by a single injection technique and by means of a standard clearance technique in children.


A comparison between diazepam, dixyrazine, opripramol and placebo in anxiety states.


Assay of serum and whole blood folate by a modified aseptic addition technique.


Quantitative analysis of lipid classes.

Quantitative analysis of lipid classes.


Biosynthesis of pregnane derivatives.

Biosynthesis of pregnane derivatives.


Antigen identification in Goodpasture's syndrome.


Lymphoma-like lymph node changes in Kaposi's sarcoma. Two additional cases.


The history of the Royal Australasian College of Surgeons, 1920-1935.


Renal transplantation in the rat: details of a technique.


Suture-line neoplastic recurrence following large-bowel resection.


Techniques for the surgical management of colon cancer.


Ulcerative colitis, autoimmune epiphenomena, and colonic cancer.


Occurrence of malignancy in immunodeficiency diseases. A literature review.


Radioelectrocardiographic supervision of patients with recent myocardial infarction during rehabilitation. Communication on method.


Insulin biosynthesis in experimental hereditary diabetes.


Principles of skin replacement in the hand.


The free graft.

The free graft.


The use of skin flaps in hand injuries.

The use of skin flaps in hand injuries.


Finger tip injuries.

Finger tip injuries.


Thumb injuries.

Thumb injuries.


Degloving injuries.

Degloving injuries.


Treatment of electrical burns.

Treatment of electrical burns.


Some observations on the autografting of digits and upper limb replantation.


The Millard "crane flap" for acute hand injuries.


Experience with computerized electrocardiograms.


The structure and function of the skin of the hand.


The pharmacist and the physician.

The pharmacist and the physician.


Chikungunya and dengue: a case of mistaken identity?


The folk healer: calling and endowment.

The folk healer: calling and endowment.


The regulation of French Midwifery during the Ancien régime.


Tarantism or hysteria? An American case of 1801.


Psychopathic ward. Lithograph by Robert Riggs, ca. 1939.


Chest wounds in ancient sculpture.

Chest wounds in ancient sculpture.


A curious parallel: coincidence or borrowing?


Enterotoxigenic Escherichia coli isolated from patients with severe cholera-like disease.


Effect of calcium concentration on inhibitors of hemagglutination of rubella virus.


Wall-defective microbial variants: terminology and experimental design.


Resistance to intracellular infection.

Resistance to intracellular infection.


Rubella: the challenge of its control.

Rubella: the challenge of its control.


Evaluation of qualitative hemagglutination test for antibodies to Treponema pallidum.


Bacterial overgrowth.

Bacterial overgrowth.


Dr. Alton Ochsner: 40 years of wisdom.

Dr. Alton Ochsner: 40 years of wisdom.


Stimulation of germinal centers by L-asparagine amidohydrolase (EC .3.5.1.1).


Cytotoxic factor from peritoneal cells: site of action.


Cultivation of mouse bone marrow cells. I. Growth of granulocytes.


Host responses to "normal" microbial flora in germ-free mice.


Hemolysis during filtration through micropores: a scanning electron microscopic and hemorheologic correlation.


Platelet-microvasculature interaction during one passage through the dog lung.


RecA + -dependent mutagenesis occurring before DNA replication in UV- and  -irradiated Escherichia coli.


An air-drying method for preparing metaphase chromosomes from the spermatogonial cells of rats and mice.


Genetic recombination in Escherichia coli. IV. Isolation and characterization of recombination-deficiency mutants of Escherichia coli K12.


Tyrosine tRNA precursor molecule polynucleotide sequence.


Natural rabbit antibody against rodent pancreas.


The granulocytic in vitro growth potential of foetal liver.


The malaria problem in Cameroon in 1969. (A demographic perspective).


In memory of Professor Lhotka, member of editorial board.


Alexander Fleming's Nobel Prize eve.

Alexander Fleming's Nobel Prize eve.


John Hunter on inflammation.

John Hunter on inflammation.


Shock in myocardial infarction--medical treatment.


The lung in shock. Alveolar-capillary gas exchange in the shock syndrome.


A modified technique of external dacryocystorhinostomy.


Guarded thermal sclerostomy.

Guarded thermal sclerostomy.


The histopathology and ultrastructure of congenital, central corneal opacity (Peters' anomaly).


Testing for congenital syphilis in interstitial keratitis.


Determination of carbon-12, carbon-13 isotopic abundances and nitrogen-carbon ratios in biological substances by proton-reaction analysis.


Hyperbaric perfusion of the isolated larynx. An autoradiographic study.


Electromyographic and visual evaluation of recurrent laryngeal nerve anastomosis in dogs.


Electron microscopic identification of virus-like particles in laryngeal papilloma.


The ultrastructure of the pathologic stria vascularis and spiral prominence in man.


Treatment of acute blunt laryngeal injuries.


Vestibular dysfunctions after midline lesions in the brain stem of the cat.


Neurological classics XXXIX. Studies of cerebellar cysts. Structure, pathogenesis, and relation to angiomatosis retinae. (Lindau).


Walton C. Finn. M.D. 1904-1971.

Walton C. Finn. M.D. 1904-1971.


Introduction of the second Carl G. Hartman lecturer, M. C. Chang.


Changes in pituitary responsiveness to luteinizing hormone-releasing hormone (LH-RH) in anestrous ewes pretreated with estradiol benzoate.


Biomedical engineering in the United States.


Long-term function of canine autograft kidneys that had been preserved extracorporeally for twenty-four hours: preservation with hypothermia and pulsatile perfusion of specially treated plasma.


On defining the pulmonary extravascular water volume.


The locus of initial calcification in cartilage and bone.


On extra-osseous actions of parathyroid hormone and calcitonin.


Osseous avascular necrosis associated with renal transplantation.


Amylase isoenzymes.

Amylase isoenzymes.


Radioisotopes as applied to diagnostic hematology.


An unusual case of tuberculosis in a medieval leper.


Genetics: Genetics Society of America.

Genetics: Genetics Society of America.


Application of sequential pattern-recognition technique to medical diagnostics.


Diuretics. II. Clinical considerations.

Diuretics. II. Clinical considerations.


The logical basis of medimatic diagnosis.


Automated system for medical data processing.


Freud's first publications in America.

Freud's first publications in America.


Effect of carbon monoxide exposure on human sleep and psychomotor performance.


Ultrastructural changes in sarcosomes after exposure of adult Calliphora erythrocephala to lethal and sub-lethal high temperatures.


A rapid method for the visual determination of size distribution of spheres from the size distribution of their sections.


Preparing chromosomes for scanning electron microscopy without enzymic digestion.


Origin of chloramphenicol particle protein.


Abortive infection of Escherichia coli strain W by T2 bacteriophage.


Genetic control of bacteriophage M13 DNA synthesis.


Complete primary structure of phage lysozyme from Escherichia coli T4.


Amino acid sequence of T2 phage lysozyme.


The P22 bacteriophage DNA molecule. I. The mature form.


The P22 bacteriophage DNA molecule. II. Circular intracellular forms.


Inactivation of Haemophilus influenzae transforming DNA by spleen acid deoxyribonuclease.


RNA codons and protein synthesis. 15. Dissimilar responses of mammalian and bacterial transfer RNA fractions to messenger RNA codons.


Regulation of messenger RNA synthesis in Escherichia coli.


Messenger RNA synthesis during amino acid starvation in Escherichia coli.


Synthesis of a specific messenger RNA during amino acid starvation of Escherichia coli.


Level of tryptophan messenger RNA in Escherichia coli.


Effects of amino acid starvation upon constitutive tryptophan messenger RNA synthesis.


Studies on proteins and protein complexes of muscle by means of proteolysis. V. Fragmentation of light meromyosin by trypsin.


Cholinesterase levels in uremic man and renal allografted man.


The fate of Mycobacterium leprae in CBA mice.


The effect of cinanserin (antiserotonin agent) on canine renal allografts.


A new regional perfusion system.

A new regional perfusion system.


The effect of lymphocyte depletion by continuous flow centrifugation in canine renal allotransplants.


Renin and erythropoietin release in renal allotransplantation states.


Delayed wound closure based on bacterial counts.


The incidence of "leukovirus" in cultured human hematopoietic cells.


Relationship between rejection and organ blood flow in sensitized and nonsensitized cardiac and renal grafts.


Effects of heparin and steroids on hyperacutely rejected renal xenografts.


Energy-rich phosphates and glucose metabolism in early endotoxin shock.


Properties of bacteriophage T4 mutants defective in gene 30 (deoxyribonucleic acid ligase) and the rII gene. In Escherichia coli K-12 strains infected with phage T4 which is defective in gene 30 [deoxyribonucleic acid (DNA) ligase] and in the rII gene (product unknown), near normal levels of DNA and viable phage were produced. Growth of such T4 ligase-rII double mutants was less efficient in E. coli B strains which show the "rapidlysis" phenotype of rII mutations. In pulse-chase experiments coupled with temperature shifts and with inhibition of DNA synthesis, it was observed that DNA synthesized by gene 30-defective phage is more susceptible to breakdown in vivo when the phage is carrying a wild-type rII gene. Breakdown was delayed or inhibited by continued DNA synthesis. Mutations of the rII gene decreased but did not completely abolish the breakdown. T4 ligase-rII double mutants had normal sensitivity to ultraviolet irradiation.


Recent therapeutic developments in lung cancer.


Cold--man's assiduous remedy.

Cold--man's assiduous remedy.


History of the Medical Society of the District of Columbia.


Rebel with a cause.

Rebel with a cause.


Was Neanderthal man rickety?

Was Neanderthal man rickety?


Malaya and Singapore revisited.

Malaya and Singapore revisited.


Uses of Australian orchids by Aborigines and early settlers.


Kodak RP-SU X-Omat Subtraction Film.

Kodak RP-SU X-Omat Subtraction Film.


Identification of lymphatic vessels after intra-arterial injection of dyes and other substances.


The manitoba medical review. A brief history.


Studies on experimental defibrination. I. Microcirculatory observations.


Distinguished pioneer physician.

Distinguished pioneer physician.


The reasons for our existence. Reprint: January 1903.


The renal lesion of angiokeratoma corporis diffusum (Fabry's disease): report of three cases including some electron microscopic findings.


Voluntary sterilization: a world view.

Voluntary sterilization: a world view.


Bibliography of scientific works of the Faculty of Medicine, Charles University at Hradec Králové 1969.


Marine fungi from tropical America and Africa.


The cardiac donor.

The cardiac donor.


Needle biopsy of primary malignant bone tumors.


Christian Fenger, 1840-1902.

Christian Fenger, 1840-1902.


The past and present of medical education.


Olan Robert Hyndman 1899-1966.

Olan Robert Hyndman 1899-1966.


James W. Stephens 1920-1966.

James W. Stephens 1920-1966.


Byron Stookey 1887-1966.

Byron Stookey 1887-1966.


Henry Alsop Riley 1887-1966.

Henry Alsop Riley 1887-1966.


Lewis J. Pollock 1886-1966.

Lewis J. Pollock 1886-1966.


Russell Brain 1895-1966.

Russell Brain 1895-1966.


Louise Eisenhardt 1891-1967.

Louise Eisenhardt 1891-1967.


American Neurological Association. List of members, 1967-1968.


The replication cycle of RNA bacteriophages.


The translation of viral messenger RNA in vitro.


Beetle transmission of plant viruses.

Beetle transmission of plant viruses.


Safe care of IPPB machines.

Safe care of IPPB machines.


Plasma renin responses to hemorrhage after cardiac autotransplantation.


Amelioration of insulin resistance in obese mice.


A report of the Lung Transplantation Workshop--1970.


Clinical experience with viable frozen human skin and a frozen skin bank.


Detection and management of tracheal stenosis following cuffed tube tracheostomy.


Surgical management of the complications of sepsis involving the aortic valve, aortic root, and ascending aorta.


A consideration of operative methods for correction of endocardial cushion defect.


Improved microscopy of Mycoplasma in vitro. Techniques were developed for continuous microscopic observation of mycoplasmata growing in vitro in Rose chambers by using an inverted phase microscope. The methods permitted direct microscopic observation of undisturbed growth of mycoplasmata in liquid medium. Inocula of mycoplasmata were passed through 0.22-mum filters before culture to provide a suspension of discrete particles. The sequential growth of Mycoplasma pneumoniae was followed from points or single straight lines, with development of branching, a net-like confluence of filaments, large bodies occurring in the center of developing colonies, and finally coccoid forms. Other species of Mycoplasma which did not attach as readily to glass could be observed also by inverted phase microscopy. Umbonation of colonies (a "friedegg" appearance) occurred in liquid medium, indicating that this appearance was not due simply to interaction with the agar medium, but may reflect a qualitative difference in growth patterns between center and periphery. For growth on solid medium, direct observation of colonies in uncovered plates of agar medium was made by using inverted phase microscopy. This was found helpful in detecting small colonies and in observing relationships between colonies.


Comparison of two plating media for the isolation of Erysipelothrix rhusiopathiae from enrichment broth culture. Sodium azide-crystal violet-agar and a modified blood-azide (MBA)-agar were used to isolate Erysipelothrix rhusiopathiae from swine tissues. There was no significant difference in the number of isolations. However, 78% of the isolants from modified blood-azide medium required only 24 hr of incubation, whereas all of the isolants obtained on sodium azide-crystal violet medium required 48 hr.


Bacteriophage types and O antigen groups of Escherichia coli from urine. Urinary strains of Escherichia coli from seven geographical regions were typed serologically for O-specific antigens and with phages capable of lysing the majority of urinary isolated. The O antigen groups 4, 6, 75, 1, 50, 7, and 25 were the common ones found. Of the 454 cultures tested, 66.1% were phage typable and 65.2% were serotypable with the 48 antisera employed. Also, 71.6% of the cultures for which an O group could be determined were phage typable. Furthermore, of those seven O-antigen groups implicated in urinary tract infection, 80.2% exhibited a phage pattern. Various phage types were found within an O-antigen group, and, although one phage type associated a high percentage of the time with one O-antigen group, no correlation was observed between other O-antigen groups and phage types. Studies with bacteriuric patients by phage typing showed the presence of two strains of E. coli within an O-antigen group. Serogrouping and phage typing of fecal isolates of E. coli revealed the presence of some O-antigen groups and phage types also found as predominant types among urinary isolates. Phage typability correlated highly with hemolysis of human erythrocytes. Elevated temperatures of incubation and a chemical curing agent were used to enhance typability of cultures refractory to the typing phages. Phage typing, due to its rapidity, ease, and ability to distinguish strains of E. coli within an O-antigenic group, is suggested as a possible method by which a better insight into the epidemiology of urinary tract infections may be obtained.


Biotin production and utilization in a sewage treatment lagoon. Biotin, in a sewage oxidation lagoon also receiving potato processing wastes, was observed to increase two logs during the summer period of waste stabilization and then to decline to near earlier concentrations. Three organisms, Aerobacter aerogenes, Chlorella vulgaris, and Thiocapsa floridana, were at least partially responsible for these fluctuations; the latter two organisms were associated with biotin utilization and the former with biotin production. Since copious quantities of biotin are produced in these waste treatment facilities, the vitamin may act as a useful indicator of microbial action on certain organic molecules, especially in domestic and industrial wastes such as those from municipalities and potato and sugar beet processing plants. Furthermore, the presence of biotin in rivers and streams may be indicative of the discharge of incompletely stabilized wastes from these sources.


Evaluation of the enterotube system for identification of members of the family Enterobacteriaceae. The Enterotube system was evaluated, in parallel with conventional bacteriological procedures for the identification of members of the family Enterobacteriaceae, by using bacterial strains from a variety of clinical specimens and from stock cultures. Excellent agreement between the two test systems was obtained with the following reactions: hydrogen sulfide, indole, Simmons' citrate, glucose, and lactose. Agreement was not as good (<85%) with the urea, phenylalanine deaminase, and dulcitol reactions. The Enterotube lysine decarboxylase test was unsatisfactory. The Enterotube method will correctly identify strains of the family Enterobacteriaceae approximately 50% of the time; if identification only as Klebsiella-Enterobacter-Serratia group is needed, the method will be correct 85% of the time. On the basis of this evaluation, the Enterotube system appears to be both simple and rapid for the presumptive identification of these bacteria. Because of the limited usefulness of the lysine decarboxylase test, the results obtained by this test system are less reliable than those obtained by conventional methods.


Certain biological effects of lead upon the animal organism.


Interrelationship of biochemical responses to the absorption of inorganic lead.


[The Right Honourable Godfrey Martin Huggins. First viscoust Malvern of Rhodesia and Bexley].


A suppressor in the genome of phage T4 inhibiting phenotypic expression of mutations in genes 46 and 47.


The fifth chromosome histocompatibility types of mouse strains Hg-Hu and C3Hf-A.


Inhibition of insulin release by endogeneous insulin in vitro.


Effect of CoCl 2  on the cells of endocrine pancreas in the snake (Natrix n. natrix).


The effect of dialysis on the carbohydrate intolerance of chronic renal failure.


The immunogenicity of human HL-A haplotypesas measured by skin graft survival times and mixed leukocyte reactions. The immunogenicity of the haplotypes in 30 families was measured by survival of skin grafts between selected paired family members. These families were genotyped for HL-A using 57 selected cytotoxic alloantisera defining HL-A 1-12 as well as other nondefined specificities. Mixed leukocyte reactions were also studied in this series and the correlations between the mixed leukocyte reactions with skin graft survival times, individual HL-A specificities, and the number of incompatible HL-A alleles are reported. Comments concerning the interpretation and quantitation of the mixed leukocyte reactions are discussed.


The nature of the colicin K receptor of Escherichia coli Cullen. Cell walls of E. coli strains B and Cullen contain specific receptors for colicin K and for the T2, T6, and C16 phages. The receptors for the bacteriocin and the T6 virus are located in the outer layers of the cell wall of these microorganisms and are absent in their cytoplasmic membrane. The receptors for colicin K, phage T2, and the T6 and C16 viruses differ in their stability toward enzymes and chemical reagents. Their specificity must therefore be determined by different chemical groupings. The colicin K receptor is inactivated by certain proteolytic enzymes and by reagents which combine with tryptophan. It is concluded therefore that proteins or peptides containing this amino acid are essential for biological activity of the receptor.


The location of a membrane-associated protein complex in human and bovine erythrocyte ghosts.


Regulation of the immune system by synthetic polynucleotides. 3. Action on antigen-reactive cells of thymic origin. Polyadenylic-polyuridylic acid complexes, a potent adjuvant to the immune response, were tested for action on thymic-influenced and bone marrow-derived lymphocytes in model systems deficient in one or the other of these cells. Adult mice, thymectomized at birth or mice treated with heterologous antithymocyte serum produced 90-95% fewer splenic rosette-forming cells than normal mice in response to an injection of sheep erythrocytes. Intravenous injection of complexes of homoribopolynucleotides, polyadenylic and polyuridylic acids, poly A:U with SRBC restored immunologic competence to NTx- or ATS-treated mice such that they produced normal or near normal levels of splenic RFC. In addition, injection of poly A:U enabled NTx mice to reject allogeneic skin grafts at the same rate as control mice with an intact thymus. Further reduction in residual thymocytes by combining neonatal thymectomy with ATS treatment reduced the number of anti-SRBC RFC induced by poly A:U. Lethally irradiated mice which received SRBC, excess bone marrow cells, and as few as 40,000 thymic lymphocytes were stimulated by poly A:U to produce RFC. No adjuvant effect was observed when irradiated mice received excess thymic lymphocytes and low doses of bone marrow cells with poly A:U. The results suggested that the adjuvant action of poly A:U was exerted on the thymic-influenced, antigen-reactive cell and that restoration of immunocompetence to NTx- or ATS-treated mice was caused by amplification of a small number of residual antigen-reactive cells which were influenced by the thymus in utero before thymectomy, or which survived treatment with ATS.


A study of fixation of early amphibian embryos for electron microscopy.


Recent advances in the field of microsurgery.


How to process Medicare and insurance forms more efficiently.


What to do when the infant is jaundiced.


A bibliography of cryptococcosis.

A bibliography of cryptococcosis.


Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.


Formation of antibiotics.

Formation of antibiotics.


Sequential activation of clotting factors during blood coagulation.


Correlation between humoral antibody and regression of tumours induced by feline sarcoma virus.


Inhibiting effect of some antimalarial substances on glucose-6-phosphate dehydrogenase.


13 C NMR spectra of lecithin vesicles and erythrocyte membranes.


Effect of infection with trypanosomes on the development of experimental allergic neuritis in rabbits.


Significance of surface potential measurements on lipid monolayers during action of phospholipase A on lecithins.


Perceptual fading of a stabilized cortical image.


Iridescent corneas in fishes.

Iridescent corneas in fishes.


Lead accumulation by wild oats (Avena fatua) in a contaminated area.


Effects of cyprenorphine hydrochloride on sensory reinforcement in the rat.


Effect of an RNA-rich extract on acquisition of a one-way avoidance response in rats.


Histoplasma farciminosum, the aetiological agent of equine cryptococcal pneumonia.


Suspected pulmonary nocardiosis in a duck.


Deficient resistance to Coccidioides immitis following intravenous vaccination. II. Evidence against an immune tolerance mechanism.


The effect of high salt concentration on fidelity of translation by Escherichia coli ribosomes.


Gram negative colonization of the respiratory tract in postoperative patients.


Optometric utilization of objective, standardized, perceptual tests.


Severely impaired consciousness in myxedema--a review.


Left ventricular performance in dogs with selective coronary embolization with mercury.


Microbiology and hydrophilic contact lenses.


Bibliography of visual science dissertations and theses: British universities, 1965-68.


The internal compliance of ventilators.

The internal compliance of ventilators.


Clinical regional cerebral blood flow measurement.


An overdose of Parstelin.

An overdose of Parstelin.


Does constant volume IPPV produce hypoxaemia?


Investigation of the changes in acid-base equilibrium which occur in neonates and small infants as a result of intermittent positive pressure ventilation performed during general anesthesia.


Endotracheal suction.

Endotracheal suction.


Aspergillus infection complicating cardiac transplantation. Report of five cases.


Potential hepatotoxicity of copper in recurrent hemodialysis.


Early diagnosis of small peripheral lung cancer--cytologic diagnosis of very fresh cancer cells obtained by the TV-brushing technique.


Shiga bacillus dysentery acquired in Nicaragua.


Automated cytology. A symposium by correspondence. 8. Patterns of cell population.


The use of the crushing condenser for photometric and other cytologic studies on histologic sections.


Epidermoid carcinoma of the lip after renal transplantation. Report of two cases.


Neuroendocrine aspects of male reproduction.


Sperm capacitation and fertilization in mammals.


Macromolecules in oocyte maturation.

Macromolecules in oocyte maturation.


Spermatozoan morphology and zygote formation in nematodes.


Drug reactions XV. Methotrexate, psoriasis and the liver.


Gamete aging and its consequences.

Gamete aging and its consequences.


The immunogenesis of the multiple sclerosis plaque.


Hairy cell leukemia.

Hairy cell leukemia. The clinical findings in 12 patients with an unusual type of leukemia have been reviewed. The leukemic cell cannot be identified with any of the normal hematopoietic cells or with any of the lymphomas and may best be referred to by the descriptive term "hairy cell", which describes its appearance, most clearly seen on phase contrast microscopy. The patients were all males and the major clinical features were enlargement of the liver and spleen, with little lymph node enlargement. Hematologic findings in most patients have been anemia and thrombocytopenia with the characteristic abnormal cells present in the peripheral blood and bone marrow. The disease most often runs an indolent course and has shown little or no response to a variety of forms of treatment.


An unusual radical vulvectomy.

An unusual radical vulvectomy.


Crush injury of the leg. (Case P29).

Crush injury of the leg. (Case P29).


Ventricular aneurysm and septal defect complicating acute myocardial infarction: early surgical correction.


Small cell tumors of the thyroid. A clinicopathologic study.


Studies on cellular proliferation in human leukemia. VII. Cytokinetic behavior of neoplastic cells in a patient with reticulum cell sarcoma in a leukemic phase.


Cyclic chemotherapy in lymphoma.

Cyclic chemotherapy in lymphoma.


Edgar Zwilling; an appreciation.

Edgar Zwilling; an appreciation.


Toward a general theory of visual adaptation.


Orientation discrimination in normal and light-deprived rabbits.


The anatomical organization of the visual system of the rabbit.


Background and historical perspectives.

Background and historical perspectives.


Studies of the acute effects of the atomic bombs.


Establishment of ABCC.

Establishment of ABCC.


Study populations, dosimetry, and pathology study program.


Malignant lymphoma.

Malignant lymphoma.


The histological distribution of the blood group substances in man as disclosed by immunofluorescence. IV. The ABH antigens in embryos at the fifth week post fertilization.


The microbiology of fish and fishery products--a progress report.


The Adansonian taxonomy of some cannery flavobacteria.


The microbial associations in British fresh sausages.


Production of flavour-enhancing materials by streptomycetes: screening for 5'-nucleotides.


The leakage of cations and amino acids from Staphylococcus aureus exposed to moist heat, phenol and dinitrophenol.


Optimum growth parameters of lactic streptococci used for the production of concentrated cheese starter cultures.


Observations on the microflora of vacuum packed sliced cooked meat products.


Response of spores and vegetative cells of Bacillus spp. in a hydrocarbon-water system.


A rapid, inexpensive bacterial count technique using agar droplets.


The microbial flora of the rumen of cows fed hay and high cereal rations and its relationship to the rumen fermentation.


Transport and storage of faeces for bacteriological examination.


A rapid test for biodegradability of PVC film by pseudomonads.


Effects of posture and exercise on the pulmonary extravascular water volume in man.


Cardiac output and arterial blood gases during exercise in pubertal boys.


Morphology of distal airways in the human lung.


Localization of group A streptococci and particles of titanium dioxide in arthritic lesions in the rabbit.


Insulin treatment of latent and potential diabetics during pregnancy.


The effects of viewing college football, basketball and wrestling on the elicited aggressive responses of male spectators.


Swimming in small laboratory animals.

Swimming in small laboratory animals.


Microflora of raw cacao beans.

Microflora of raw cacao beans.


Albumin synthesis measurement by means of an improved two tracer method in patients with chronic renal failure. Effects of low protein diet.


Recent advances in the management of low urinary obstruction in the newborn.


Exstrophy of the bladder.

Exstrophy of the bladder.


Recent advances in the management of myelomeningoceles.


Past, present and future of neonatal surgery and neonatal surgical units.


Recent advances in the surgery of the diaphragm in the newborn.


Intralumenal intestinal obstruction.

Intralumenal intestinal obstruction.


Recent advances in the management of anorectal abnormalities.


Renal cystic disease in childhood.

Renal cystic disease in childhood.


In memoriam. George M. Wyatt, M.D. 1908-1970.


Innominate artery compression syndrome in children.


Ventilatory patterns and pulmonary complications after upper abdominal surgery determined by preoperative and postoperative computerized spirometry and blood gas analysis.


PHA-induced rejection of parental skin grafts by F1 hybrids.


Fractionation of cells infiltrating the skin allograft in mice on a discontinuous albumin gradient.


The epidemiology of cholera: past and present.


The history of Asiatic cholera in the United States.


The control of cholera.

The control of cholera.


Cholera: diagnosis and treatment.

Cholera: diagnosis and treatment.


Recollections of cholera at the turn of the century.


Hemodynamic studies after open heart surgery utilizing thermal dilution.


Orthotopic transplantation of the rat heart and lungs.


Physiologic evaluation of the renal cadaver allograft.


The Lazar House Library in Norwich.

The Lazar House Library in Norwich.


Dr. John W. Francis.

Dr. John W. Francis.


Sutureless vascular anastomosis in kidney homotransplantation. A study on animal experiments.


Role of phytohemagglutinin in the survival of pancreatic allotransplants in the dog.


A comparison of antisera against thymocytes, lymphocytes and subcellular fractions in rats.


Autograft sutures in fascial tissue.

Autograft sutures in fascial tissue.


Auxiliary liver allotransplantation in dogs--an improved technic and effectiveness of antihistaminic drugs in preventing outflow block.


Phases of thymineless death in Escherichia coli 15 TAU.


R factors of Escherichia coli strains causing urinary tract infections, their types of transfer factors and differences in their transmissibility to Citrobacter and Salmonella typhimurium recipient strains.


Properties of Escherichia coli grown in deuterated media.


The localization of IgA and IgE globulins in the palatinal tonsils, nasal mucous membrane and nasal polypi.


Eeva Jalavisto. 1909-1966.

Eeva Jalavisto. 1909-1966.


Quantitative studies on immunofluorescence in allergic changes of old and young animals.


Sterol absorption--from lumen to liver.

Sterol absorption--from lumen to liver.


Cellular receptors. effect of anti-alloantiserum on the recognition of transplantation antigens. The possibility that a rat alloantiserum DA anti-Lewis possesses similar recognition structures for Lewis transplantation antigens, as do DA immunocompetent cells, was investigated by raising an antiserum against this alloantiserum in (Lewis x DA)F(1) hosts. This antiserum, as well as one provoked by injecting DA lymphoid cells, was active against recognition structures for Lewis antigens of DA immunocompetent cells. The anti-(DA anti-Lewis) antiserum displayed the same degree of specificity as was found previously for anti-recognition structure sera prepared by injecting parental strain lymphoid cells into F(1) hosts. Since the activities of antisera raised against cell-bound receptors or against the antigen-binding region of an immunoglobulin were indistinguishable, it was concluded that the functional part of cell-associated receptors might be structurally similar to the variable portion of an immunoglobulin.


Studies on antibody-producing cells. 3. Identification of young plaque-forming cells by thymidine- 3 H labeling. Mice injected with sheep RBC and then, 4 days later with thymidine-(3)H, were sacrificed on the day of thymidine-(3)H injection or 1 or 2 days later. Hemolytic antibody plaque preparations were made of cells from the draining lymph nodes by a thin-plating procedure permitting collection of isolated PFC for electron microscopic examination and radioautography. Of cells obtained on the day of thymidine-(3)H injections, 65% of the labeled PFC were in the lymphocytic category, in comparison with 13% found previously in the entire population of such cells. The remaining 35% were plasmablasts in early stages of differentiation. Cells obtained 1 day after the thymidine-(3)H injections showed a shift to a majority of labeled cells in the plasmacytic category. Also, the plasmablasts were substantially more differentiated than those of the previous day, and some mature plasma cells were now seen. The labeled PFC obtained on day 2 gave no indication of further differentiation. Cells of rabbit lymph nodes labeled in vitro with thymidine-(3)H showed a range of labeled PFC. The majority were in the plasmacytic category, including some mature plasma cells. The data from the experiments with in vivo labeling suggest a direct differentiation from antibody-synthesizing lymphocytes to plasma cells. Further, the in vivo experiments indicated that differentiation from nascent lymphocyte to plasma cell could be essentially completed within 1 day.


Biological characterization of an immunosuppressant from group A streptococci. A component in extracts of Group A streptococci suppresses antibody formation in mice against heterologous erythrocyte and protein antigens. Large doses are not toxic and repeated injection does not change its effectiveness. It is most effective when injected 1 or 2 days before antigen and it is not suppressive when given after antigen. The active factor occurs as a large polydisperse complex and activity can be increased 10- to 25-fold by filtration through Sepharose 2B. Both direct (gammaM) and indirect (gammaG) antibody-forming cells are suppressed in primary and secondary responses. Injection before a primary response does not reduce memory cell development. It increases rather than depresses the "background" antibody-forming cells to sheep erythrocytes, and is equally effective if injected intraperitoneally or intravenously. Ribonuclease increases activity while deoxyribonuclease has no effect. Proteases destroy immunosuppressive action.


Immunohistochemical study of creatine phosphokinase in pathological human muscle.


Necrosis of infant hamster cerebellum due to a tick-borne encephalitis virus.


Histocompatibility studies in a closely bred colony of dogs. 3. Genetic definition of the DL-A system of canine histocompatibility, with particular reference to the comparative immunogenicity of the major transplantable organs. The segregation of the canine DL-A leukocyte group antigen(s) b, c, d, e, f, g, h, k, l, and m has been traced in 141 consecutive matings in the Cooperstown Colony of beagles. All of the leukocyte antigen(s) were regularly transmitted en bloc from parent to offspring, with no instance of independent segregation. A total of 23 haplotypes, including six different DL-A antigen patterns (gl, bkhfm, bkcd, e, be, fgl) was observed. 31 different DL-A phenotypes were observed in a population of 100 mongrel dogs. A number of statistically significant positive and negative associations between individual DL-A antigenic components occurred in this population. The results support the concept of the DL-A system as a complex immunogenetic system governed by a single region (or locus) of an autosomal pair of chromosomes. Studies of skin, kidney, heart, and liver allografts in the Cooperstown Colony indicated that the longest allograft survivals occur under genetically and serologically defined conditions of donor-recipient DL-A compatibility. Skin and renal allografts generally behaved in parallel fashion, while cardiac allografts survived for longer periods of time (MST = 47.1 days) than kidneys (MST = 28.1 days) or skin (MST = 25.1 days) under conditions of DL-A identity. Heart transplants were rejected at a more rapid rate than kidney, however, in DL-A-incompatible donor-recipient combinations. Liver transplants were accorded the longest survival time (MST = 76.2 days) under conditions of DL-A identity, but were rejected at a rapid rate (MST = 5 days) in DL-A-incompatible nonlittermate donor-recipient pairs. The results provide further evidence that the DL-A system is the principal system of histocompatibility in the canine species. The differences in survival of different organs under similar conditions of donor-recipient DL-A compatibility suggest, however, the existence of a number of unknown variables which may also be capable of significantly affecting allograft behavior.


The glia-neuronal interaction: some observations.


The cellular basis of behavior in Aplysia.


The vestibular nuclei and spinal motor activity.


Is pain a specific sensation?

Is pain a specific sensation?


Responses of photoreceptors.

Responses of photoreceptors.


Electrophysiology of visual and perceptual activity.


Quantitative hedonism.

Quantitative hedonism.


Neurochemistry of reward and punishment: some implications for the etiology of schizophrenia.


Short-term storage and long-term storage in recall.


Current status and future prospects for research in psychosomatic medicine.


Military psychiatry and changing systems of mental health care.


Reversion of frameshift mutations stimulated by lesions in early function genes of bacteriophage T4. Temperature-sensitive (ts) mutants representative of a number of genes of phage T4 were crossed with rII mutants to allow isolation of ts, rII double-mutant recombinants. The rII mutations used were characterized as frameshift mutations primarily on the basis of their revertability by proflavine. For each ts, rII double mutant, the effect of the ts mutation on spontaneous reversion of the rII mutation was determined over a range of incubation temperatures. A strong enhancement in reversion of two different rII mutants was detected when they were combined with tsL56, a mutation in gene 43 [deoxyribonucleic acid (DNA) polymerase]. Three other mutants defective in gene 43 enhanced reversion about fourfold. Two mutations in gene 32, which specifies a protein necessary for DNA replication, enhanced reversion about 5-fold and 18-fold, respectively. Two additional mutations in gene 43 and two in gene 32 had no effect. Fivefold and threefold enhancements in reversion were also found with mutations in genes 44 (DNA synthesis) and 47 (deoxyribonuclease), respectively. No significant effect was found with mutations in seven additional genes. The results of other workers suggest that frameshift mutations arise from errors in strand alignment during repair synthesis occurring at chromosome tips. Our results show that such errors can be enhanced by mutations in the DNA polymerase, the gene 32 protein, and the enzymes specified by genes 44 and 47. This implies that these proteins are employed in the repair process occurring at chromosome tips and that mutational errors in these proteins can lead to loss of ability to recognize and reject strand misalignments.


Marker rescue with ultraviolet-inactivated influenza virus. Influenza Al/CAM virus undergoes abortive growth in FL cells, whereas X-3311, a recombinant virus clone from the cross between CAM and NWS, is capable of complete replication in FL cells and plaque formation on FL monolayers (FL-marker). Clone 1-5C-4 cells are permissive for both viruses. When either clone 1-5C-4 or FL cells, which were mixedly infected with both ultraviolet-irradiated X-3311 and active CAM viruses, were plated on FL monolayers, infective centers far exceeding in number those expected from the surviving X-3311 virus were observed, i.e., the FL-marker of the irradiated parent was rescued. The significantly lower radiation sensitivity of FL-marker than that of infectivity indicates that only part of the genome is responsible for the FL-marker. The capability of X-3311 virus to produce NP antigen and its infectivity were lost at the same time when the former was assayed under the condition of low multiplicity of infection. This suggests that only infectious virus is capable of producing NP antigen and that no stepwise inactivation of the viral genome occurs. It is also suggested that any lethal ultraviolet damage inflicted upon the viral genome prevents the expression of the portion of the genome coding for NP antigen.


Replication and recombination in ligase-deficient rII bacteriophage T4D. Deoxyribonucleic acid replication and genetic recombination were investigated after infection of Escherichia coli with ligase-deficient rII bacteriophage T4D. The major observations are: (i) deoxyribonucleic acid synthesis is discontinuous, (ii) the discontinuities are more slowly repaired than in wild-type infection, (iii) host ligase is required for viability, and (iv) genetic recombination is increased.


Partial suppression of bacteriophage T4 ligase mutations by T4 endonuclease II deficiency: role of host ligase. Endonuclease II-deficient, ligase-deficient double mutants of phage T4 induce considerably more deoxyribonucleic acid (DNA) synthesis after infection of Escherichia coli B than does the ligase-deficient single mutant. Furthermore, the double mutant can replicate 10 to 15% as well as wild-type T4, whereas the single mutant fails to replicate. When the E. coli host is also deficient in ligase, the double mutant resembles the single mutant. The results indicate that host ligase can substitute for phage ligase when the host DNA is not attacked by the phage-induced endonuclease II.


Identity of genes coding for soluble and structural dihydrofolate reductases in bacteriophage T4. By recombination between bacteriophage T4 wh2, a dihydrofolate reductaseless mutant, and T6, I have prepared T4 wh(T6), a T4 strain which codes for the T6-specific soluble dihydrofolate reductase. This strain has the heat sensitivity of T6, not T4, which provides direct evidence that the wh gene codes for both the soluble dihydrofolate reductase and the structural dihydrofolate reductase which is a constituent of T-even phage tail plates.


Successful treatment of end-stage renal failure "Down-Under and in Northlands".


U.K. multicentric study. Comments by the other participants.


Observations on bacterial sensitivities to nalidixic acid and critical comments on the 6-centre survey.


Observations on a seasonal increase in one laboratory in urinary infections with Escherichia coli resistant to two or more antibacterial drugs.


Multicentric study of sensitivities of urinary tract pathogens.


Curative and suppressive treatment of P. falciparum malaria with oral and parenteral Fansidar.


Current concepts in the medical treatment of duodenal ulcer.


Selection of treatment for patients with duodenal-ulcer disease.


Surgical management of peptic ulceration recurring postoperatively.


Diagnosis of stomal ulceration.

Diagnosis of stomal ulceration.


Surgical management of emergency complications of duodenal ulcer.


Surgical management of acute gastric and duodenal stress ulcers.


Jaundice as a complication of duodenal ulcer.


The usefulness of vagotomy in treatment for gastric ulcer.


The surgical management of gastric ulcer.


Esophageal reflux ulceration. Causes and surgical management.


Management of gastrointestinal dysfunction after gastric surgery.


Peptic ulceration in childhood.

Peptic ulceration in childhood.


Gastric motility and ulcer surgery.

Gastric motility and ulcer surgery.


Gastroscopy of peptic ulceration.

Gastroscopy of peptic ulceration.


Primary hyperparathyroidism and peptic ulcer.


Trends in anesthesia for surgical treatment of acid peptic disease.


The spores of Badhamia gracilis (Myxomycetes).


The use of labelled fibrinogen in the diagnosis of venous thrombosis.


Formation, viscosity and degradation of artificial thrombi after cadaveric-donor kidney transplantation.


Problems in the measurement of ventricular volumes.


Some notes on the history of congenital heart disease.


Ventricular intracradiac shunting mechanisms in congenital heart disease.


Use of videometry and electronic data-processing for hemodynamic investigations by angiographic techniques.


Assessment of myocardial function in congenital heart disease.


A system for on-line computer analysis of data during heart catheterization.


Sedimentation of small viruses at very low concentrations.


Specialized transduction of the Salmonella hut operons by coliphage lambda: deletion analysis of the hut operons employing lambda-phut.


Genetic control of the DNA maturation in the process of phage morphogenesis.


The process of infection with coliphage T7. V. Shutoff of host RNA synthesis by an early phage function.


Appearance of transducing activity in P1-infected Escherichia coli.


Chain initiation during polypeptide synthesis in cell-free bacterial systems programmed with a plant viral messenger. Initiation with N-acetylated aminoacyl-tRNAs on adjacent codons.


Chain initiation during polypeptide synthesis in cell-free bacterial systems programmed with a plant viral messenger. The formation of N-acetylphenylalanylisoleucyl-tRNA on the messenger-ribosome complex.


Howard Culbertson, Harvey Hospital Surgeon.


Structural studies on the M-antigen from two mucoid mutants of Salmonella typhimurium.


On the two cultures in medicine as reflected by the holism of Bela Schick.


Oral versus inhaled corticosteroid for chronic asthma.


In memoriam. George S. Frauenberger, M.D. 1906-1969.


In memoriam. Aaron D. Spielman, M.D. 1902-1970.


In memoriam. George F. Harsh, M.D. 1897-1970.


Current status of tissue typing for allotransplantation.


Prophylactic synovectomy of the joints of the rheumatoid hand. Clinical trial with 4 to 8-year follow-up.


Jose Verocay. Pioneer Uruguayan neuropathologist.


Dr. N.H. Myers.

Dr. N.H. Myers.


Dr. Leslie Abeles.

Dr. Leslie Abeles.


Injection injury of the sciatic nerve.

Injection injury of the sciatic nerve.


Peripheral nerve grafts.

Peripheral nerve grafts.


Reconstructive procedures after irreversible nerve damage in the upper extremity.


Neuroradiological diagnosis of sellar and parasellar lesions.


Correlative functional anatomy of the optic chiasm. 1969.


Orbital decompression for exophthalmos.

Orbital decompression for exophthalmos.


Remarks concerning intracranial aneurysmal surgery.


Surgery of sellar and parasellar tumors.


Hypophysectomy in endocrine disorders.

Hypophysectomy in endocrine disorders.


Intracranial microsurgery.

Intracranial microsurgery.


Surgery of vascular lesions of the spinal cord with the microsurgical technique.


The surgery of spinal cord tumors.

The surgery of spinal cord tumors.


Concomitant craniocerebral and spinal trauma, with special reference to the cervicomedullary region.


Lesions of the cauda equina.

Lesions of the cauda equina.


Report of the ad hoc committee on nomenclature of neurosurgical operations.


Congress of Neurological Surgeons, Inc. Membership roster July 1970.


Anatomical features of nerve trunks in relation to nerve injury and nerve repair.


The electromyogram and conduction velocity studies in peripheral nerve trauma.


Peripheral nerve repair.

Peripheral nerve repair.


Internal neurolysis in the treatment of peripheral nerve injuries.


Wallace B. Hamby: a biographical sketch.


Antipsoriatic activity of retinoic acid (vitamin A acid).


Immunosuppressive properties of corticosteroids. I. Aldosterone as immunosuppressant in skin allograts in rats.


Immunosuppressive properties of corticosteroids. II. Interactions of antilymphocyte serum with corticosteroids in adrenalectomized rats.


Organs transplantation in pig with different portal and caval venous drainage.


Prolongation of survival of allogeneic skin grafts by L-asparaginase. Preliminary report.


Modern trends in experimental organ transplantation.


Skin cover in hand injuries.

Skin cover in hand injuries.


A tribute to O.W.B.

A tribute to O.W.B.


Quality of dental care. Its measurement, description and evaluation.


The development of the professions.

The development of the professions.


Community health--a dental school affair. The service function of dental schools and their programs in preventive and community dentistry.


Ultrastructural analysis of mitotic spindle elongation in mammalian cells in vitro. Direct microtubule counts. The mitotic spindle of many mammalian cells undergoes an abrupt elongation at anaphase. In both cultured rat kangaroo (strain PtK(1)) and Chinese hamster (strain Don-C) fibroblasts, the distance from pole to pole at metaphase doubles during anaphase and telophase. In order to determine the organization and distribution of spindle microtubules during the elongation process, cells were fixed and flat embedded in Epon 812. Selected cells were photographed with the phase-contrast microscope and then serially sectioned perpendicular to the major spindle axis. Microtubule profiles were counted in selected sections, and the number was plotted with respect to position along the spindle axis. Interpretation of the distribution profiles indicated that not all interpolar microtubules extended from pole to pole. It is estimated that 55-70% of the interpolar microtubules are overlapped at the cell equator while 30-45% extend across the equator into both half spindles. This arrangement appeared to persist from early anaphase (before elongation) until telophase after the elongation process. Although sliding or shearing of microtubules may occur in the spindle, such appears not to be the mechanism by which the spindle elongates in anaphase. Instead, our data support the hypothesis that spindle elongation occurs by growth of prepositioned microtubules which "push" the poles apart.


Study of the dynamics of pulmonary veins by a new method. Preliminary report.


In vitro aggregation of mixed embryonic kidney and nerve cells. Influence on macromolecular synthesis. The possible role of nerve on growth of embryonic parenchymal organs such as kidney was explored by measuring macromolecular synthesis (DNA, RNA, and protein and three enzymes) in aggregates of mixed suspensions of cells from dissociated chick embryo kidney and nerve tissue. One and one-half to threefold increments in net synthesis of the three different types of macromolecules were observed in the mixed aggregates of kidney and nerve cells as compared with those of single organs or mixtures of kidney with nonneural cells. The addition of nerve-growth factor (NGF) did not significantly affect the results. Increased incorporation of label was paralleled by increases in chemically measured DNA and protein, suggesting an increase in growth in the mixed kidney-nerve aggregates compared with those of single tissues. Measurements of survival rate did not indicate increased cell stability in the mixed aggregates. The activities of three enzymes, acid phosphatase, alkaline phosphatase, and lactic dehydrogenase, were also enhanced two to four times in cultures of kidney plus nerve cells. Morphologic studies indicated a high degree of reorganization of tubular structures within the reaggregates of kidney cells alone or in those mixed with nerve. In addition, radioautographs of thymidine-(3)H-labeled cells in the aggregates showed a high level of DNA synthesis in the reformed tubular cells. Electron micrographs revealed the presence of large numbers of nerve fibers containing microtubules in the mixed cell aggregates. The data suggest a significant role for nerve in the growth processes of embryonic parenchymal organs.


Stability of cells fixed with glutaraldehyde and acrolein.


Formation and origin of basal lamina and anchoring fibrils in adult human skin. The purpose of this investigation was to study the formation and origin of basal lamina and anchoring fibrils in adult human skin. Epidermis and dermis were separated by "cold trypsinization." Viable epidermis and viable, inverted dermis were recombined and grafted to the chorioallantoic membrane of embryonated chicken eggs for varying periods up to 10 days. Basal lamina and anchoring fibrils were absent from the freshly trypsinized epidermis before grafting although hemidesmosomes and tonofilaments of the basal cells remained intact. Basal lamina and anchoring fibrils were absent from freshly cut, inverted surface of the dermis. Beginning 3 days after grafting, basal lamina was noted to form immediately subjacent to hemidesmosomes of epidermal basal cells at the epidermal-dermal interface. From the fifth to the seventh day after grafting, basal lamina became progressively more dense and extended to become continuous in many areas at the epidermal-dermal interface. Anchoring fibrils appeared first in grafts consisting of epidermis and viable dermis at five day cultivation and became progressively more numerous thereafter. In order to determine the epidermal versus dermal origin of basal lamina and anchoring fibrils, dermis was rendered nonviable by repeated freezing and thawing 10 times followed by recombination with viable epidermis. Formation of basal lamina occurred as readily in these recombinants of epidermis with freeze-thawed, nonviable dermis as with viable dermis, indicating that dermal viability was not essential for synthesis of basal lamina. This observation supports the concept of epidermal origin for basal lamina. Anchoring fibrils did not form in recombinants containing freeze-thawed dermis, indicating that dermal viability was required for anchoring fibrils formation. This observation supports the concept of dermal origin of anchoring fibrils.


On the octagonality of the nuclear pore complex.


Isolation of nucleoli. A method that combines high yield, structural integrity, and biochemical preservation.


Renal pelvic tumors.

Renal pelvic tumors.


Unusual peroxidase-positive granules in the developing rat submaxillary gland.


Electronically generated sections from serial sections.


Dual origin of intimal cells in cardiac-allograft arteriosclerosis.


Effect of cesarean section on neonatal body water spaces.


General model for the structure of all myosin-containing filaments.


The assessment of organ function--the testis.


Chromosomal localization of the heterochromatic region 16qh(.76) linked to alpha-haptoglobin in man.


Improved method for simultaneous counting of Cr 51  and I 125  in blood volume measurement.


Interpretation of linkage in somatic cell hybrids.


Activation of a central noradrenergic projection to cerebellum.


Regeneration of porphyropsin in vivo.

Regeneration of porphyropsin in vivo.


Carboxymethylpachymaran, a new water soluble polysaccharide with marked antitumour activity.


Terpenoid amines as insect juvenile hormones.


Infection of schistosomes with Gram-positive cocci.


Temperature optimum of non-sulphur bacteria from a spring at 90C.


Potential cocarcinogenicity of sodium hypochlorite.


Hormonal pregnancy tests and neural tube malformations.


Effect of Avena sativa on cigarette smoking.


Possible relationship between smoking and peptic ulcer.


Orientation-specific masking of letter features.


Organochlorine pesticide residues in harbour porpoises from the Bay of Fundy region.


Earliest indian human remains found in a Late Stone age site.


The morphogenesis of renal parenchymal maldevelopment (renal dysplasia).


A double-blind study of the effects of levodopa in Parkinson's disease.


An international cooperative study, societies of paediatric nephrology, and Henry L. Barnett.


Heritable cystic disorders of the kidney. The mythology of polycystic disease.


Studies of renal function in children with chronic hydronephrosis.


The immunologic basis of human renal disease.


The renal regulation of acid-base balance and the disturbances noted in renal tubular acidosis.


Epidemiology and natural history of urinary tract infection in school age children.


Mechanisms of edema and the use of diuretics.


Systemic arterial hypertension in pediatric practice.


The pathophysiology of renal insufficiency. On the functional transformations in the residual nephrons with advancing disease.


Treatment of renal failure in children.

Treatment of renal failure in children.


Renal function and renal metabolism.

Renal function and renal metabolism.


Bibliography of current literature on allergy.


The 1967 Nobel Prizes for Physiology or Medicine.


Family planning in West Virginia Public Health Clinics.


Transplantation of callus-invaded Kiel bone to defect pseudarthroses. An experimental study.


BCG-osteomyelitis and -osteoarthritis as a complication following BCG-vaccination.


Angiography in tumors of the extremities.


Urinary pH values in African, Asian and European subjects in Kampala.


Clausius Henry Mastin--internist-genitourinary surgeon.


Dextroamphetamine-responsive behavior disorder in school children.


Marijuana, memory, and perception.

Marijuana, memory, and perception.


Administration of marijuana to heavy and casual marijuana users.


Health insurance: not if, or when, but what kind?


Medicaid practitioner abuses and excuses vs. counterstrategy of the New York City Health Department.


Public health: then and now: social and health problems are inseparable.


The difficulty of appraisal of streptokinase treatment of myocardial infarction.


[Luigi Maggiore, 1888-1970].

[Luigi Maggiore, 1888-1970].


Cancer of the breast--the last twenty-five years.


Pimozide as maintenance treatment in chronic schizophrenia.


Bronchodilator effect of oral salbutamol in asthmatics treated with corticosteroids. In a double-blind trial the effect on ventilatory function of oral salbutamol (in two different doses) and a placebo were studied in 12 patients with chronic asthma receiving regular maintenance treatment with prednisolone. Salbutamol in a dose of 4 mg four times daily, given for a period of four weeks, produced a sustained and statistically significant increase in peak expiratory flow rate over the pretreatment recordings. This effect was not observed with a lower dose of salbutamol (2 mg four times daily) or with a placebo. Salbutamol in the higher dose would seem to be an effective and safe oral bronchodilator that can be recommended for the treatment of mild or moderate asthma. The duration of treatment in this study was, however, limited to four weeks, and it is not known whether effective bronchodilatation would be maintained if the drug were given for longer periods.


Nitrimidazine compared with metronidazole in the treatment of vaginal trichomoniasis. A new substituted nitroimidazole, nitrimidazine (Naxogin), is compared with the established drug, metronidazole (Flagyl), for the treatment of vaginal trichomoniasis in a randomized double-blind trial. Nitrimidazine cured 39 (68%) out of 57 patients and showed no undesirable effects other than nausea in one patient. Metronidazole cured 51 (89%) out of 57 patients and also caused nausea in one patient; this cure rate corresponds with that previously reported in other trials. In the recommended dosage nitrimidazine is inferior to metronidazole, but is sufficiently effective to be useful in cases of intolerance to metronidazole.


Clofibrate and fat embolism: a double-blind trial of the effects of clofibrate on sequelae to injury. In a double-blind prospective trial clofibrate was found not to influence significantly the clinical and laboratory indices thought to reflect the state of fat embolism in patients with fractures.


Quantitative estimation of the proportion of perikaryal surface area covered with boutons--a possibility to distinguish different nerve cell populations.


Ischaemic myocardial damage in chronic renal failure.


Dimensions of change in the drug industry.


Anticoagulant and thrombolytic drugs. II. Clinical aspects.


Inhibition of the lymphocytotoxicity reaction by sodium salicylate.


Fifty years of dental research at the National Bureau of Standards.


Oral problems in the elderly.

Oral problems in the elderly.


[Neuropsychiatric geriatrics: diagnostics and therapy].


Salt and water excretion after renal transplantation.


Nucleotides adjacent to the 5'-terminus of bacteriophage R17 RNA.


Diffusion coefficient of DNA in solution at "zero" concentration as measured by electron microscopy.


Studies on the 5'-terminus of turnip yellow mosaic virus RNA.


Messenger activity of nascent ribosomal RNA.


Magnesium ion dependence of free and polysomal ribosomes from Escherichia coli.


Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli.


Transport systems for galactose and galactosides in Escherichia coli. II. Substrate and inducer specificities.


Viral and bacterial protein syntheses in MS2-infected Escherichia coli cells.


Enzymic joining of polynucleotides. 3. The polydeoxyadenylate-polydeoxythymidylate homopolymer pair.


Enzymic joining of polynucleotides. IV. Formation of a circular deoxyadenylate-deoxythymidylate copolymer.


An in vitro transversion by a mutationally altered T4-induced DNA polymerase.


Interaction of polyvinyl sulfate with ribosomes.


Effect of cyanide and carbon monoxide on the replication of bacterial DNA in vivo.


Studies on the bacteriophage fl. I. Alkali-induced disassembly of the phage into DNA and protein.


Genetic mapping of the regulator and operator genes of the lac operon.


Survival of mouse skin isografts from leukemic donors.


Purified Roswell Park cultured antilymphocytic globulin RPMI-CALG: preparation and clinical trial.


The effect of PCO (yeast respiratory stimulator) on treated and untreated dog renal allografts.


Immunologic considerations of fetal survival in mice.


Temperature and contamination dependent freeze-etch images of frozen water and glycerol solutions.


Fine structure of the spermatozoon of the Australian lungfish Neoceratodus forsteri (Krefft).


Electron microscope observations on thick filaments in striated muscle from the lobster Homarus americanus.


Failure to isolate mycoplasmas from upper samll intestine of man.


Adjuvant effect of endotoxin on primary antibody formation in irradiated rats.


Personality and organization theory: a review of some conceptual literature.


Spleen colony-forming capacity of bone marrow from mice bearing fibrosarcoma.


Effectiveness of adrenal corticosteroids in the management of severe bacterial infections.


Chlorpromazine, clotiapine and thioridazine--a comparative clinical trial on Bantu psychotic patients.


Eric Dyke, M.B., Ch.B., (Univ. Glasg.).

Eric Dyke, M.B., Ch.B., (Univ. Glasg.).


Victor Harold Turnbull, M.B., B.Ch. (Rand), R.C.P. (Lond.), R.C.S. (Eng.).


The use and efficacy of mucolytic agents.


W.H.D. Trubshaw, M.R.C.P. (Eng.), L.R.C.P. (Lond.) F.R.C.S. (Eng.).


Structural and functional changes in cardiac transplants.


Clinical experience in transplantation of the human heart: report of ten cases.


The first heart transplant in man: historical reexamination of the 1964 case in the light of current clinical experience.


Clinical and experimental observations in heart transplantations.


Rejection of the transplanted heart.

Rejection of the transplanted heart.


Cardiac homotransplantation in man.

Cardiac homotransplantation in man.


Cardiac transplantation: current status.


Hypothermia and high pressure oxygen in whole organ storage.


Summation-symposium on heart transplantation.


Organ preservation in transplantation.

Organ preservation in transplantation.


Kidney preservation for transplantation. I. Twelve-hour storage in rabbits.


Lung, liver, and kidney preservation in transplantation.


The stabilized autoperfusing heart-lung preparation as a vehicle for extracorporeal preservation.


Enzyme levels in the effluent perfusates from frozen canine kidneys.


Transplantation Society: Eastern Hemisphere Membership List.


Transplantation Society: Western Hemisphere Membership List.


Erythropoietin release from preserved kidneys.


Discussion of recurrent glomerulonephritis.


Antilymphocyte globulins in kidney transplantation: effects on glomerular disease.


Role of humoral antibodies in rejection of renal allografts.


Interpretations of transplanted kidney morphology.


Anti-DNA antibodies and renal lesions of patients with systemic lupus erythematosus.


Virus-induced immune-complex-type glomerulonephritis.


Induction of renal disease with antisera to group A streptococcal membranes.


Glomerulonephritis in renal transplants.


Tissue antigens of man and chimpanzees; their role in xenografting.


Cross-species transplantation in embryonic and neonatal animals.


Immunologic aspects of cross-species transplantation.


Specific suppression of renal allograft rejection by treatment with antigen and antibody.


Studies on heterologous antilymphocyte serum in mice. VII. Optimal cellular antigen for induction of immunologic tolerance with antilymphocyte serum.


Response of mice to xenografts of skin.

Response of mice to xenografts of skin.


Effect of immunosuppression and cellular antigen on xenograft survival.


Clinical heterotransplantation of the liver.


Sir Peter Brian Medawar.

Sir Peter Brian Medawar.


Mechanism and modification of rejection of heterografts between divergent species.


The cochlear blood vessels in guinea pigs of different ages.


Chronic frontal sinusitis treated by autogenous osteoplasty.


Pseudoepiglottis and pseudoglottis from pedicle skin flap. A modification of Asai's technique.


Histochemical localization of acetylcholinesterase (AChE) activity in the inner ear.


Stroke and occlusive cerebrovascular disease: review of surgical results in 383 patients.


Cancer of the tongue: review of thirteen-year experience--1955-1968.


Renal function, recovery and rehabilitation after kidney transplantation: an analysis of 30 consecutive patients.


Management of patients with thermal injuries.


Dendrite bundles in lamina IX of cat spinal cord: a possible source for electrical interaction between motoneurons?


Accuracy of cardiac output computers.

Accuracy of cardiac output computers.


Influence of the agonal period on the postmortem metabolic state of the heart. A problem in cardiac preservation.


Preoperative extracorporeal irradiation of the blood as adjunctive immunosuppression in human renal transplantation.


Twenty-four hour renal preservation and perfusion utilizing platelet-rich plasma.


Localization of urinary infection.

Localization of urinary infection.


Effects of histamine acid phosphate and pentagastrin on gastric secretion in normal human subjects and in patients with various gastroduodenal diseases.


Piromidic acid, a new antibacterial agent: antibacterial properties.


In vitro antimicrobial activity of a new semisyntheti penicillin, BL-P 1654 (6-(R- -(guanylureido) phenylacetamido)-penicillanic acid).


Adreno-corticosteroids in the treatment of bacterial sepsis: a double-blind study with pharmacological doses.


Immunity in typhoid fever: evaluation of live streptomycin-dependent vaccine.


Nifuratrone, a new nitrofuran: in vitro and in vivo activities.


Methicillin-resistant staphylococcemia: bacteriological failure of treatment with cephalosporins.


Intravenous sodium colistimethate therapy of urinary-tract infections: pharmacological and bacteriological studies.


Preclinical assessment of the antibacterial activity of nebramycin factor 6.


Comparison of methods for typing Proteus mirabilis as applied in an epidemiological study of infections in a nursery for newborns.


Susceptibility of Nocardia species to modern antimicrobial agents.


Immunogenicity of cefazolin.

Immunogenicity of cefazolin.


Clinical studies of cefazolin in the surgical field.


Synthetic medium for susceptibility testing.


Susceptibility of Serratia, Pseudomonas, and Enterobacter to acetic acid.


Antibiotic susceptibility of bacteroides species.


Antibiotic susceptibility of nonpigmented Serratia marcescens.


Clinical features and diagnosis of anaerobic pleuropulmonary infections.


Magnetic tape recorders for low-frequency biological signals: a discussion.


Extraction-replication techniques for electron microscope studies of biological tissues.


Localization of theta alloantigens in mouse brain by immunofluroescence and cytotoxic inhibition.


Diacetyl production by Propionibacterium shermanii in milk cultures.


Characterization of some of the acidic products of glucose fermentation by a large-cell strain of Escherichia coli.


Characterization of mycoplasmas isolated from the great apes.


Synchronous growth of Staphylococcus aureus induced by amino acid and thymine starvation.


Recent advances in diagnosis and treatment of hemophilia and related diseases.


Serum enzymes.

Serum enzymes.


Growth rate of Escherichia coli at elevated temperatures: limitation by methionine. When Escherichia coli growing in minimal medium is shifted from 37 C to any temperature in the range 40 to 45 C, the growth rate immediately assumes a new, lower value, characteristic of that temperature. The decrease is shown to be due, in several strains, to decreased activity of the first enzyme of the methionine pathway, homoserine trans-succinylase, which thus appears to be more heat-sensitive than any other essential enzyme in the cell. This sensitivity does not involve progressive denaturation of the enzyme; rather, the response to a shift of temperature, in either direction, is immediate and reversible.


Growth rate of Escherichia coli at elevated temperatures: reversible inhibition of homoserine trans-succinylase. The preceding paper (10) showed that the growth of Escherichia coli is slowed, without killing, at 40 to 45 C, and that in the several strains tested the cause is a decrease in the activity of homoserine trans-succinylase. These temperatures are now shown to inhibit the enzyme directly, in crude extracts and after partial purification. The effect is rapid and is immediately reversible, unlike the progressive and slowly reversible changes of conventional denaturation.


Ampicillin-resistant mutants of Escherichia coli K-12 with lipopolysaccharide alterations affecting mating ability and susceptibility to sex-specific bacteriophages. Mutations from moderate (class I) to high (class III) ampicillin resistance in a male and a female strain of Escherichia coli K-12 have been found to be accompanied by surface alterations, first demonstrated as hindrance in the formation of mating pairs. These changes have now been studied with the ribonucleic acid phage MS2, and especially with the "female-specific" phage phiW. Several class III mutations in male and female strains were found to make the cells susceptible to phage phiW and to reduce their abilities to form mating pairs. Spontaneous phage phiW-resistant mutants isolated from class III strains were found also to have acquired changes in ampicillin resistance and ability to form mating pairs. One mutant had reverted to parental class I type in all three properties. Lipopolysaccharides (LPS) prepared from phiW-sensitive class III strains inactivated the phage in vitro, whereas LPS from phage-resistant strains had no effect. Carbohydrate analyses of LPS preparations showed that two class III mutants, compared to their parental strains, had lost significant parts of the rhamnose, galactose, and glucose from the LPS. One of the phage phiW-resistant mutants showed a partial restoration of its carbohydrate composition. Other phiW-resistant mutants showed, instead, further losses of carbohydrates in their LPS. It is suggested that genes exist which simultaneously mediate a female-specific mating site, ampicillin resistance, and the receptors for phage phiW.


Repression of 3-deoxy-D-arabinoheptulosonic acid-7-phosphate synthetase (trp) and enzymes of the tryptophan pathway in Escherichia coli K-12. Mutant strains of Escherichia coli K-12 have been isolated in which the synthesis of 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase (trp) is partially constitutive. The mutation causing derepression is closely linked to aroH [the structural gene for DAHP synthetase (trp)] and occurs in a locus designated aroJ. The aroJ mutation is not recessive in an aroJ(+)/aroJ(-) diploid strain, as the synthesis of DAHP synthetase (trp) is still derepressed in this strain. On the basis of its close linkage to aroH and its continued expression in an aroJ(+)/aroJ(-) diploid, it is postulated that aroJ is an operator locus controlling the expression of the structural gene aroH. In support of this conclusion, the synthesis of anthranilate synthetase is still normally repressible in aroJ(-) strains, whereas, in trpR(-) strains, both DAHP synthetase (trp) and anthranilate synthetase are synthesized constitutively. The synthesis of DAHP synthetase (trp) remains repressible in an operator-constitutive mutant of the tryptophan operon. In two trpS mutants which possess defective tryptophanyl transfer ribonucleic acid synthetase enzymes, neither DAHP synthetase (trp) nor anthranilate synthetase derepress under conditions in which the defective synthetase causes a decrease in growth rate. On the other hand, an effect of the trpS mutant alleles on the level of anthranilate synthetase has been observed in strains which are derepressed for the synthesis of this enzyme, because of a mutation in the gene trpR. Possible explanations for this effect are presented.


Effect of iododeoxyuridine upon conjugation and the fate of transferred deoxyribonucleic acid in Escherichia coli K-12. The incorporation of 5-iododeoxyuridine (IUdR) into Escherichia coli K-12 deoxyribonucleic acid (DNA) has been found to decrease significantly the viability of female strains A288 and JC411(r) but to have only minor effect upon their ability to act as conjugational recipients and to perform recombination after conjugation. In contrast, IUdR incorporation into male strain HfrC appears to interfere with both chromosome transfer and genetic recombination. By using IUdR to densitylabel female DNA, and carrying out large-scale matings with (3)H-thymidine-labeled male cells, we examined the fate of transferred DNA. After a 30-min mating, the T6-sensitive male cells were lysed, and the DNA of the merozygotes and remaining female cells was isolated. Initial centrifugation of this DNA in a CsCl gradient showed that the male and female DNA species were associated. The nature of this association of the parental DNA species was determined by formaldehyde denaturation followed by CsCl centrifugation. Denaturation of DNA isolated immediately after T6 lysis gave a peak of radioactivity banding at the density of light single-stranded DNA. However, denaturation of DNA isolated after T6 lysis and dilution of the cells into fresh medium, exhibited peaks of radioactivity banding at positions corresponding to single-stranded, density-labeled DNA. The results indicate that recombination after conjugation in E. coli takes place by a breakage-and-reunion mechanism. The process of recombination can be separated into two stages. In the first stage, the donor and recipient DNA molecules become associated. The second stage consists of the formation of phosphodiester bonds between the donor and recipient segments comprising the recombinant molecule.


Immunochemical comparison of L-asparaginases from Serratia marcescens and Escherichia coli. Immunochemical properties of l-asparaginases from Serratia marcescens and Escherichia coli were compared by means of quantitative precipitin reactions and quantitative microcomplement-fixation reactions employing homologous and heterologous antisera. The enzyme preparations used in these tests produced one arc of precipitate upon immunoelectrophoresis. According to our results with the precipitin and micro-complement fixation reactions, both enzymes are related immunochemically. On the basis of these tests, distinct structural differences exist between the two enzymes. From results with the micro-complement fixation test, we estimated that the two enzymes could differ by 10 to 15 amino acid residues.


Mutants of Escherichia coli sensitive to antibiotics. Mutants of Escherichia coli sensitive to the antibiotic synergistin A, an inhibitor of protein synthesis, were isolated. These mutants were pleiotropic, being also sensitive to a large number of unrelated antibiotics and to lysis by detergents. These pleiotropic responses indicated that the mutations affected cell wall or membrane synthesis. Consequently, selection for antibiotic-sensitive mutants constitutes a useful means for isolating cell wall or membrane mutants.


Deoxyribonucleic acid transferred from ultraviolet-irradiated excision-defective Hfr cells of Escherichia coli K-12. Deoxyribonucleic acid (DNA) transfer from (3)H-thymine-labeled Hfr cells has been measured by determining the amount of radioactivity remaining after selective lysis of the donor cells in the mating mixture. DNA transfer was less effectively reduced by ultraviolet irradiation of excision-defective Hfr cells than was the yield of recombinants. The buoyant density of DNA transferred from unirradiated and irradiated Hfr cells was equivalent to that of double-stranded DNA. Mating-dependent DNA synthesis in the recipient has been measured by mating Hfr cells deficient in thymidine kinase with irradiated thymine-requiring F(-) cells in the presence of (3)H-thymine. The extent of such DNA synthesis approximated the amount of DNA transferred from unirradiated donors. Neither DNA transfer nor mating-dependent DNA synthesis could be reliably measured when both parents were irradiated. It is proposed that transferred Hfr DNA is replicated in the recipient and that this replication still occurs when the Hfr DNA contains dimers.


Comparison of the morphology and deoxyribonucleic acid composition of 27 strains of nitrifying bacteria. The gross morphology, fine structure, and per cent guanine plus cytosine (GC) composition of deoxyribonucleic acid of 27 strains of nitrifying bacteria were compared. Based on morphological differences, the ammonia-oxidizing bacteria were separated into four genera. Nitrosomonas species and Nitrosocystis species formed one homogenous group, and Nitrosolobus species and Nitrosospira species formed a second homogenous group in respect to their deoxyribonucleic acid GC compositions. Similarly, the nitrite-oxidizing bacteria were separated into three genera based on their morphology. The members of two of these nitrite-oxidizing genera, Nitrobacter and Nitrococcus, had similar GC compositions, but Nitrospina gracilis had a significantly lower GC composition than the members of the other two genera.


Identification of the forms of vitamin B 6  present in the culture media of "vitamin B 6  control" mutants. An Escherichia coli mutant resistant to isoniazid (WG497) contained 0.6 mumole of extracellular pyridoxamine and pyridoxamine phosphate in the early stationary phase. A suppressed lysine mutant (AT1024) contained 1.4 mumoles of pyridoxal phosphate under the same conditions. The internal concentration of vitamin B(6) was one-half of normal for AT1024 and increased fivefold for WG497.


Enriched selection of dominant mutations: histidine operator mutations. In the course of selection of bacteria with derepressed levels of histidine biosynthetic enzymes, it was found that when mutagen-treated cells were spread on a selective medium without allowing intervening growth to occur, the frequency of operator mutants obtained was dramatically increased. This may be useful as a general enrichment for operator or other dominant mutations.


Location of three genes concerned with the conversion of 2,3-dihydroxybenzoate into enterochelin in Escherichia coli K-12. Mutants of Escherichia coli K-12 unable to synthesize the iron-sequestering compound, enterochelin, from 2,3-dihydroxybenzoate have been isolated and divided into three classes on the basis of tests for enzymatic complementation. The genes affected (designated entD, entE, and entF) have been mapped by cotransduction and are located at about minute 14 on the E. coli genome. They were found to be closely linked to other genes (entA, entB, and entC) concerned with enterochelin biosynthesis and a gene (fep) concerned with the uptake of the iron-enterochelin complex. No detectable diffusible intermediate in the formation of enterochelin from 2,3-dihydroxybenzoate was formed by cell extracts of mutants carrying mutations in the entD, entE, or entF genes.


Inhibition of arginyl-transfer ribonucleic acid synthetase activity of Escherichia coli by arginine biosynthetic precursors. The arginine biosynthetic precursors, ornithine, citrulline, and argininosuccinate, inhibit arginyl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.13, arginine: soluble RNA ligase, adenosine monophosphate) activity in the in vitro attachment assay system. Ornithine is the most potent, argininosuccinate is next, and citrulline is least effective. The implications of these results are discussed in relation to arginyl-tRNA synthetase activity and the level of the arginine biosynthetic enzymes during conditions of restricted and unrestricted supply of arginine to cells.


An efficient preparation of isocodeine from codeine.


Neurogenic abnormalities of ventricular repolarization.


Secretagogue activity of a dimeric analog of the gastrin peptide.


Regulation of the biosynthesis of guanosine 5'-monophosphate: evidence for one operon.


Peptide chain growth of  -galactosidase in Escherichia coli.


On the process of cellular division in Escherichia coli. I. Asymmetrical cell division and production of deoxyribonucleic acid-less bacteria.


Effect of bacteriophage infection upon the specificity of leucine transfer RNA for RNA codewords.


Orientation of transcription for the galactose operon as determined by hybridization of gal mRNA with the separated DNA strands of coliphages lambda-dg.


Dependence of the sedimentation coefficient on molecular weight of RNA after reaction with formaldehyde.


Studies on the mechanism of repression of arginine biosynthesis in Escherichia coli. 3. Repression of enzymes of arginine biosynthesis in arginyl-tRNA synthetase mutants.


Mutations creating a new initiation point for expression of the histidine operon in Salmonella typhimurium.


Studies on Escherichia coli sex factors. IV. Molecular weights of the DNA of several F' elements.


Rate of production of single-strand breaks in DNA by x-irradiation in situ.


On the autobiography of Malcolm X.

On the autobiography of Malcolm X.


Arthur Hugh Simmons, M.D., 1899.

Arthur Hugh Simmons, M.D., 1899.


Amino acid replacements of the glutamic acid residue at position 48 in the tryptophan synthetase A protein of Escherichia coli.


Creativity: hot and cold.

Creativity: hot and cold.


Structural analysis: a theory of personality based on projective techniques.


The neurology of stammering.

The neurology of stammering.


Activity and affect in early memories of adolescent boys.


Electromyographic studies of tension.

Electromyographic studies of tension.


Problems in the measurement of sleep with particular reference to the development of a motility bed.


Tubular reabsorption of sodium ion: influence of factors other than aldosterone and glomerular filtration rate. 1.


Status of amantadine in control of influenza.


Earlier case of oto-psycho-pharmacology.


Amino-acid synthesis from gases detected in interstellar space.


New type of compound with strong sweetness.


Luminescent system in a myctophid fish, Diaphus elucens Brauer.


Lesions due to complement in lipid membranes.


Physiological mechanisms for the regulation of energy balance.


Metabolic and physical control of food intake in ruminants.


Food intake and growth in the newly-born.


Protein metabolism after injury.

Protein metabolism after injury.


Radiosensitivity of in vivo mouse bone marrow cells.


Changes of serum enzyme activities in dogs receiving heterotopic cardiac allografts.


Electrocardiographic changes during the unmodified rejection of heterotopic canine heart allografts.


Heterotopic cardiac transplantation in dogs.


Angiographic changes following heterotopic cardiac transplantation in dogs.


Evaluation of tetramisole in the treatment of nematode infestations in man.


Prolongation of mouse skin allograft survival by treatment with cytosine arabinoside in combination with F 1  hybrid lymphocytes.


The metabolism of azathioprine in renal failure.


The effect of 9-butyl azathioprine on canine renal homograft survival.


Response of the draining lymph node in the early afferent arc of the allograft rejection reaction.


Studies on the lymphocytes of patients with renal homografts.


A synergistic effect of pooling individual antithymocyte sera.


Presence of both cell-mediated immunity and serum-blocking factors in rat renal allografts "enhanced" by passive immunization.


Proliferation of donor hematopoietic cells in irradiated and unirradiated host mice.


Controlled comparison of the therapeutic effect of trimeproprimine and amitriptyline.