Bacteriophage and the toxigenicity of Clostridium botulinum type C. Nontoxigenic and bacteriophage-sensitive bacterial cultures have been isolated from toxigenic Clostridium botulinum type C, strain 468C, after treatment with either ultraviolet light or acridine orange. Two bacteriophages, designated CEbeta and CE(gamma), were isolated from toxigenic strain 468C. Both of these bacteriophages were capable of infecting the nontoxigenic type C cultures, but only bacteriophage CEbeta was involved in the change from nontoxigenicity to toxigenicity.


Polyacrylamide gel electrophoresis.

Polyacrylamide gel electrophoresis. Polyacrylamide gel electrophoresis (PAGE) provides a versatile, gentle, high resolution method for fractionation and physical-chemical characterization of molecules on the basis of size, conformation, and net charge. The polymerization reaction can be rigorously controlled to provide uniform gels of reproducible, measurable pore size over a wide range. This makes it possible to obtain reproducible relative mobility (Rf) values as physical-chemical constants. Application and extension of Ogston's (random fiber) model for a gel allows for calculation of molecular volume, surface area, or radius, free mobility, and valence from RJ measurements at several gel concentrations, to calculate gel concentration for optimal resolution, and to predict behavior of macromolecules on gel gradients by computerized methods. Extension of classical moving boundary theory has been used to generate multiphasic buffer systems (providing selective stacking, unstacking, restacking, and preparative steady-state-stacking) with known operating characteristics for any pH at 0 degrees and 25 degrees C. A general strategy for isolation of macromolecules and for macromolecular mapping has been developed. Preparative scale PAGE is operational for milligram loads and feasible for gram quantities.


Metabolism of 14C-histamine in domestic animals. 3. Horse and pig.


Metabolism of 14C-histamine in domestic animals. II. Cow and sheep.


A comparison of two methods of value measurement.


A three-dimensional reconstruction of the rods in rat maxillary incisor enamel.


Acoustic impedance of pathological ears.


The Habsburg jaw.

The Habsburg jaw.


Origin of spectacles in India.

Origin of spectacles in India.


Clotting abnormalities following orthotopic and heterotopic transplantation of marginally preserved pig livers.


Interaction of host and cancer: the potential antigenicity of cancer cells, the defense mechanisms of cancer patients, and the influence of endocrine glands and aging on cancer.


Curriculum vitae Georgeanna Seegar Jones, M.D., President of the American Fertility Society, 1970-1971.


The influence of environmental and surgical stressors on susceptibility to bacterial endotoxin.


The remote changes in the endometrial histology and the cervicovaginal cytology with the long acting hormonal contraceptive.


Recent trends in cancer research.

Recent trends in cancer research.


The present approach to systematic cancer therapy.


The affective response in the perception of sound stimuli.


Application of stereologic methods to stratified gingival epithelia.


Positive carriers of violence among children: detection by speech deviations.


Detergers deterred.

Detergers deterred.


Guillemots and PCBs.

Guillemots and PCBs.


Controlling chromosome loss.

Controlling chromosome loss.


Estimation of secondary structure in ribonucleic acids.


Fanconi's anaemia in the genetics of neoplasia.


Adrenal tyrosine hydroxylase and dopamine beta-hydroxylase in spontaneously hypertensive rats.


Epidermal antigens in cutaneous dysplasia and neoplasia.


Carcinogenic effect of a dimethyl sulphoxide extract of betel nut on the mucosa of the hamster buccal pouch.


Homotransplantation of isolated epiphyseal and articular cartilage chondrocytes into joint surfaces of rabbits.


Use of hydrated polyphenylene oxide sulphonate ion exchange membranes for artificial kidneys.


Origin of apparent polyploid spermatocytes in the mouse.


Initial crystallin synthesis and lens fibre formation are independent of lens morphogenesis.


Fluorescent glucoside in the human lens.


Effect of a bright light flash on dark adaptation of human rods.


Excretion and retention of stable strontium in children.


Is the new composite cranium from Swartkrans a small robust australopithecine?


Estimation of ecological production where age of the population is unknown.


Effects of ethirimol on cytological characters in barley.


Antischistosomal action of tubercidin administered after absorption into red cells.


Principle of competitive exclusion and Drosophila.


Professor D. G. Millar.

Professor D. G. Millar.


Water gets worse.

Water gets worse.


Fire ants defended.

Fire ants defended.


Chemistry of learning.

Chemistry of learning.


Research in the Serengeti.

Research in the Serengeti.


Standing with a bent knee.

Standing with a bent knee.


Mitochondrion as a multifunctional organelle.


Some aspects of the evolution of hearing in vertebrates.


Late australopithecine from Baringo District, Kenya.


Visual sensations produced by single fast particles.


Musical tests for functional lateralization with intracarotid amobarbital.


Fourier analysis of evoked potentials and human intelligence.


Identification of prostaglandin F2-alpha released by distension of guinea-pig uterus in vitro.


Luteolysis and ovulation induced by prostaglandin F2-alpha in the hamster.


In vitro inhibition of bovine serum and pancreatic amylase by progesterone.


Binding of sheep red blood cells to a large population of human lymphocytes.


Effect of N,N-dimethyltryptamine and D-lysergic acid diethylamide on the release of 5-hydroxyindoles in rat forebrain.


3-O-methyldopa, a new precursor of dopamine.


Pathogenesis of central pontine myelinolysis. A clinical and pathological description of three cases.


A brief history of cosmetic surgery.

A brief history of cosmetic surgery.


Correction of midfacial deformities.

Correction of midfacial deformities.


Cosmetic considerations in surgery of the hand.


Reduction mammaplasty.

Reduction mammaplasty.


Cutaneous scars and cosmetic surgery.

Cutaneous scars and cosmetic surgery.


Presidential address.

Presidential address.


Dimethylacetamide, a new cryoprotective agent for platelets.


Histotypic organization of the rat retina in vitro.


The chemical regulation of ventilation.

The chemical regulation of ventilation.


Interpretation of CVP measurements.

Interpretation of CVP measurements.


Guillain-Barré syndrome in pregnancy. Respiratory paralysis complicated by a fatal tracheo-innominate artery fistula.


Hereditary congenital severe deafness syndromes.


Membrane attachment of replicating parental DNA molecules of bacteriophage M13.


Effect of charge on the determination of molecular weight of proteins by gel electrophoresis in SDS.


Stimulation of cell free polyphenylalanine synthesis by 30S ribosomal subunits in Escherichia coli.


Kinetic studies of tryptophan synthetase. Interaction of L-serine, indole, and tryptophan with the native enzyme.


Relaxation spectra of aspartate transcarbamylase. Interaction of the catalytic subunit with carbamyl phosphate, succinate, and L-malate.


Relaxation spectra of aspartate transcarbamylase. Interaction of the native enzyme with aspartate analogs.


Amino acid sequence of bovine carboxypeptidase A. Isolation and characterization of selected peptic and nagarse peptides and the complete sequence of fragment F-I.


Sedimentation behaviour of phosphoribosyladenosine triphosphate synthetase. Effects of substrates and modifiers.


Modification of the induced electromyogram in man during respiratory acidosis and alkalosis.


Blood platelet behaviour in syphilis.

Blood platelet behaviour in syphilis.


Survey of gonorrhoea practices in Great Britain. British Cooperative Clinical Group.


Direct and delayed methods of immunofluorescent diagnosis of gonorrhoea in women.


Tuberculin energy.

Tuberculin energy.


Controlled trial of metoclopramide in the treatment of flatulent dyspepsia. A double-blind cross-over trial of metoclopramide (Maxolon) against placebo was undertaken in 42 patients with flatulent dyspepsia. A highly significant difference was found in favour of the active drug (P<0.01). The time at which the drug is given in relation to the meal and onset of symptoms is probably important. It is concluded that metoclopramide is effective in the short-term treatment of these symptoms but should be started only after thorough investigations have excluded more serious disease.


The distribution of dental caries in ancient British populations. 1. Anglo-saxon period.


Controlled clinical trials and the tolbutamide controversy.


Sir James Learmonth 1895-1967.

Sir James Learmonth 1895-1967.


Crystallization of biotin carboxylase, a component enzyme of the acetyl-CoA carboxylase system from Escherichia coli.


Flavodoxin and ferredoxin of Escherichia coli.


Social schemata of emotionally disturbed boys and their male siblings.


Epics of angina.

Epics of angina.


Adjuvancy of endotoxin from heptoseless Salmonella mutants.


In vitro demonstration of suppression of the efferent site in transplantation immunity with antilymphocyte serum.


Genetic mapping of aro, pyr, and pur markers in Klebsiella pneumoniae.


Autolytic enzyme system of Clostridium botulinum. I. Partial purification and characterization of an autolysin of Clostridium botulinum type A.


Activity of thiozamin (p-(p'-aminobenzenesulfonyl)-benzaldehyde thiosemicarbazone) against Mycobacterium leprae infections in mice.


Replication and transfer of the R factor in a synchronized culture of Escherichia coli.


Relation between the cell wall composition and susceptibility of Salmonella typhimurium to anti-salmonella substance extracted from mouse spleens.


History of the Medical Society of the District of Columbia. IX. Functions of a modern medical society.


Blockade of ovulation in the immature rat by local microinjection of alpha-methyl-dopa into the arcuate region of the hypothalamus.


'Ultrashort' feedback control of follicle-stimulating hormone-releasing factor secretion.


Neuroendocrine status of old constant-estrous rats.


The William McLean Wallace Symposium on Growth and Development. Introduction.


Mechanisms for the transfer of calcium into and out of the skeleton.


William McLean Wallace: student, scholar, teacher.


The relationships between adrenal cortical steroids, parathyroid extract, and calcitonin.


Oedema.

Oedema.


Pantothenic acid in the treatment of osteoarthrosis.


Treatment of sciatica due to intervertebral disc herniation with chymoral tablets.


Renal disorders in rheumatoid arthritis.


Evaluation of reagent-impregnated strips for identification of Enterobacteriaceae.


Phagocytosis of E. coli by enzyme-treated alveolar macrophages.


Relationship of aminopyrine clearance to gastric secretion after feeding and feeding plus fat.


Immunological aspects of neoplasia: a rational basis for immunotherapy.


Chloral hydrate and warfarin interaction: clinical significance.


Anaesthesia and carbohydrate transport across cell membranes.


Gastric acid secretion and the alkaline tide.


Mechanism of action of botulinum toxin.

Mechanism of action of botulinum toxin.


Anaesthesia and cell division.

Anaesthesia and cell division.


Logical foundations of medicine.

Logical foundations of medicine.


Cryptococcal meningoencephalitis and 5-fluorocytosine.


Malaria. Epidemiology.

Malaria. Epidemiology.


Automated and programmed services in health care.


Malaria. Chemoprophylaxis and chemotherapy.


Percival's medical ethics: promise and problems.


The clinical physiology of calcium homeostasis, parathyroid hormone, and calcitonin. II.


Multiphasic testing, 1971. A socio-economic report of the Bureau of Research and Planning, California Medical Association.


Ecological studies on yeasts in the St. Lawrence River.


Ultrastructural evidence of immunological reactions during experimental coxsackievirus B-3 infection in the hibernating squirrel.


Germination and outgrowth of Bacillus thuringiensis and Bacillus alvei spores viewed by scanning electron and phase-contrast microscopy.


Effects of radiation pasteurization on Samonella. 3. Radiation lethality and the frequency of mutation to antibiotic resistance.


14th crookshank lecture syringomyelia: a radiodiagnostic and radiotherapeutic  saga.


Differences between metabolic responses to fasting in obese diabetic and obese nondiabetic subjects.


Regulation of IgM memory expression by spleen cells cultured in diffusion chambers.


Studies on the products of peptic digestion of IgA.


The cervical vertebra spreader. Evolution of a surgical instrument.


Immunological reactivity to sheep red blood cells in three congenic resistant strains of mice.


Reconstruction of the cervical esophagus by revascularized autografts of intestine. An experimental study using the Inokuchi Stapler and reporting the use of revascularized intestine to construct an artificial human larynx.


Protected exposure. A method of managing selected skin grafts.


Effects of bleomycin A2 on deoxyribonuclease, DNA polymerase and ligase reactions.


Studies on the drug resistance of Staphylococci and Escherichia coli against antibiotics. 3. Multiple-drug resistances.


Proteins and fibrinolysis in recipients of renal allografts. Studies of serum proteins, platelets, and fibrinolysis in patients after renal allografting show that a fall in plasma fibrinogen in the postoperative period can be associated with early rejection and that a rise in serum fibrin degradation products when accompanied by a fall in platelets is associated with rejection. Fibrinolysis is depressed in these patients until two months after transplantation and marked depression of fibrinolysis may precede rejection. The late coincidence of fibrinolytic depression with a rise in fibrin degradation products suggests chronic insidious rejection.


A low molecular weight ribonucleic acid synthesized by Escherichia coli in the presence of chloramphenicol: characterization and relation to normally synthesized 5 s ribonucleic acid.


Studies on 5 s RNA conformation by partial ribonuclease hydrolysis.


Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome.


Selective dissociation of free ribosomes of Escherichia coli by sodium ions.


Properties of hybrid ribosomes formed from the subunits of mesophilic and thermophilic bacteria.


Interaction between DNA and an Escherichia coli protein omega.


In vivo assay of protein synthesizing capacity of Escherichia coli from slowly growing chemostat cultures.


Growth rate of polypeptide chains as a function of the cell growth rate in a mutant of Escherichia coli 15.


X-ray and optical diffraction studies of bacterial flagella.


Structure of branch points in replicating DNA: presence of single-stranded connections in lambda DNA branch points.


Studies on polynucleotides. XCVI. Repair replications of short synthetic DNA's as catalyzed by DNA polymerases.


Studies on polynucleotides. XCVII. Opposing orientations and location of the su+3 gene in the transducing coliphages phi-80psu+3 and phi-80dsu+3su-3.


Anomalous origin of a single coronary artery.


Apparent maturation of Escherichia coli ribosomal RNA in vitro.


Experimental ovine mastitis. A pathologic study.


Bronchodilator activity of aerosols of prostaglandins E1 and E2 in asthmatic subjects.


Surgical repair of gross palatal defect.


Mycoplasmas and the evidence for their pathogenicity in man.


The tube pedicle flap in cleft palate repair.


Selecting the next neuroradiological investigation with the help of a computer.


Struggle against infection.

Struggle against infection.


The nonius horopter. I. History and theory.


In vitro cultivation procedures for parasitic helminths: recent advances.


Sensory deprivation.

Sensory deprivation.


Paramphistomiasis of domestic ruminants.


Simplified adaptor for electroelution.

Simplified adaptor for electroelution.


Detection of radioactive components in polyacrylamide gel disc electropherograms by automated mechanical fractionation.


Biomedical education as a system.

Biomedical education as a system.


Twenty-five years of rheumatology.

Twenty-five years of rheumatology.


Drug abuse in heroin users. An experimental study of self-administration of methadone, codeine, and pentazocine.


Pimozide compared with fluphenazine in schizophrenia.


Theoretical and experimental studies of conformations of polypeptides.


The benefits of cessation of smoking.

The benefits of cessation of smoking.


Donor shock and its influence on canine cadaver heart transplants.


Is IPPB therapy over-rated?

Is IPPB therapy over-rated?


New information regarding digitalis metabolism.


Management of chronic renal failure.

Management of chronic renal failure.


Temperature-jump relaxation studies on transfer ribonucleic acids. Valine and tyrosine-specific tRNAs from Escherichia coli.


Interaction of lipopolysaccharides and lipid A with complement.


The effect of temperature and ionic strength of the electrophoretic mobility of yeast mitochondrial RNA.


Nineteenth-century medical psychology. Theoretical problems in the work of Griesinger, Meynert, and Wernicke.


Eloge. Charles Donald O'Malley, 1907-1970.


Properties of F' factor deoxyribonucleic acid transferred from ultraviolet-irradiated donors: photoreactivation in the recipient and the influence of recA, recB, recC, and uvr genes. Ultraviolet (UV)-irradiated Escherichia coli K-12 F lac(+) donors transfer damaged F' factors when mated with female cells. Exposure of the zygotes to white light after mating can cause the photoreactivation of the damaged transferred F' factors. In so far as the photoreactivation is specific for pyrimidine dimers, these experiments indicate the presence of UV-induced dimers in the transferred F' factor deoxyribonucleic acid (DNA). These damaged F' factors are remarkably stable in recA recipients, having a half-life for susceptibility to photoreactivation of 1.5 to 2 hr. This is judged by the formation of Lac(+) colonies, all of which are male secondary donors. In crosses with wild type and uvrA, recB, or recC mutant recipients, however, the Lac(+) colonies are predominantly recombinants and photoreactivation is not detected. The extent of DNA synthesis resulting from transfer of F' episomes from irradiated donors suggests that a complementary strand is formed on the damaged template. Photoreactivation behavior and sedimentation properties are used to deduce properties of such damaged episomes. We conclude that the complementary strand is discontinuous directly opposite dimers in the transferred strand. This structure may be an intermediate in the recombinational event sequence in Rec(+) recipients.


Metabolism of exogenous purine bases and nucleosides by Salmonella typhimurium. Purine-requiring mutants of Salmonella typhimurium LT2 containing additional mutations in either adenosine deaminase or purine nucleoside phosphorylase have been constructed. From studies of the ability of these mutants to utilize different purine compounds as the sole source of purines, the following conclusions may be drawn. (i) S. typhimurium does not contain physiologically significant amounts of adenine deaminase and adenosine kinase activities. (ii) The presence of inosine and guanosine kinase activities in vivo was established, although the former activity appears to be of minor significance for inosine metabolism. (iii) The utilization of exogenous purine deoxyribonucleosides is entirely dependent on a functional purine nucleoside phosphorylase. (iv) The pathway by which exogenous adenine is converted to guanine nucleotides in the presence of histidine requires a functional purine nucleoside phosphorylase. Evidence is presented that this pathway involves the conversion of adenine to adenosine, followed by deamination to inosine and subsequent phosphorolysis to hypoxanthine. Hypoxanthine is then converted to inosine monophosphate by inosine monophosphate pyrophosphorylase. The rate-limiting step in this pathway is the synthesis of adenosine from adenine due to lack of endogenous ribose-l-phosphate.


Chromosomal integration of F' factors in recombination-deficient Hfr strains of Escherichia coli. In matings between F' donors and recombination-deficient Hfr recipients, we isolated progeny which transferred both episomal markers and Hfr markers early and with high frequency. A number of these progeny had two integrated sex factors. Investigation of these double Hfr strains showed that the F' nearly always integrated in a homologous region of the chromosome. In any particular mating system integration was specific as to location and direction of chromosome transfer.


Involvement of recombination genes in growth and viability of Escherichia coli K-12. We have studied the growth properties of 17 isogenic strains of Escherichia coli K-12 differing only in the recA, recB, recC, and sbcA alleles. We have observed the following. (i) All recombination deficient strains have decreased growth rates and decreased viabilities compared with recombination proficient strains. The large populations of nonviable cells in Rec(-) cultures may arise by spontaneous lethal sectoring (9). (ii) A recA mutant strain which is entirely recombination deficient and which shows high ultraviolet sensitivity and "reckless" deoxyribonucleic acid (DNA) breakdown has approximately the same growth rate and twice the viability as recB and recC mutant strains which have residual recombination proficiency, moderate ultraviolet sensitivity, and "cautious" DNA breakdown. (iii) Indirectly suppressed (sbcA(-)) recombination proficient (Rec(+)) revertants of recB and recC mutant strains have approximately normal growth rates and are three times as viable as their Rec(-) ancestors (but not as viable as rec(+) cells). We suggest the following hypothesis to account for the low viability of Rec(-)E. coli. Single-strand breaks in the DNA duplex, necessary for normal bacterial growth, may be repaired in a Rec(+) cell. Failure of Rec(-) cells to repair this normal DNA damage may lead to the observed loss of viability.


Mutants of Salmonella typhimurium with an altered leucyl-transfer ribonucleic acid synthetase. Two trifluoroleucine-resistant mutants of Salmonella typhimurium, strains CV69 and CV117, had an altered leucyl-transfer ribonucleic acid (tRNA) synthetase. The mutant enzymes had higher apparent K(m) values for leucine (ca. 10-fold) and lower specific activities (ca. twofold) than the parent enzyme when tested in crude extracts. Preparations of synthetase purified ca. 60-fold from the parent and strain CV117 differed sixfold in their leucine K(m) values. In addition, the mutant enzyme was inactivated faster than the parent enzyme at 50 C. The growth rates of strains CV69 and CV117 at 37 C were not significantly different from that of the parent, whereas at 42 C strain CV69 grew more slowly than the parent. Leucine-, valine-, and isoleucine-forming enzymes were partially derepressed when the mutants were grown in minimal medium; the addition of leucine repressed these enzymes to wild-type levels. During growth in minimal medium, the proportion of leucine tRNA that was charged in the mutants was about 75% of that in the parent. The properties of strain CV117 were shown to result from a single mutation located near gal at minute 18 on the genetic map. These studies suggest that leucyl-tRNA synthetase is involved in repression of the enzymes required for the synthesis of branched-chain amino acids.


Inefficiency of genetic recombination in hybrids between Escherichia coli and Salmonella typhosa. An Escherichia coli Hfr strain in which three negative chromosomal alleles (leu(-), arg(-), and mtl(-)) were closely linked to three positive alleles (ara(+), rha(+), and xyl(+), respectively) was employed in matings with a Salmonella typhosa recipient. The detected expression of the negative E. coli alleles in S. typhosa hybrids selected for receipt of an associated positive E. coli marker was used to determine the occurrence of haploid S. typhosa recombinants, as distinguished from stable partial diploid hybrids. At the same time, the inheritance patterns and segregation behavior of the positive alleles provided indicators of the occurrence of partial diploid hybrids. Examination of both positive and negative markers inherited by ara(+), rha(+), and xyl(-) selected S. typhosa hybrid classes indicated that relatively short E. coli chromosomal segments (generally about 4 min or less in length) were involved in recombination (haploidy), whereas rather extensive E. coli genetic segments were conserved in the diploid state. S. typhosa hybrids selected for receipt of the ara(+) marker showed a 52% incidence of leu(-) haploidy, which is probably close to being an accurate measure of recombination at the site of the ara(+) allele. S. typhosa hybrids selected for receipt of the rha(+) or xyl(+) markers showed only a 20% incidence of arg(-) or mtl(-) haploidy, respectively, but both of these hybrid classes exhibited a higher incidence of conservation of extensive E. coli diploid segments than did the ara(+) selected class. Remating of haploid S. typhosa hybrids with recombinant xyl(+)mtl(-) or rha(+)arg(-) regions resulted in higher frequencies of hybrid recovery than were observed in the initial matings. However, there was a higher incidence of partial diploidy and a lower incidence of haploidy among the hybrids obtained from these rematings.


Two mutations in the first gene of the histidine operon of Salmonella typhimurium affecting control. Two strains with mutations in the first structural gene of the histidine operon of Salmonella typhimurium were characterized. (The first structural gene specifies the first enzyme of histidine biosynthesis, phosphoribosyltransferase, which is sensitive to feedback inhibition by histidine.) One mutation, hisG3934, results in a phosphoribosyltransferase which is no longer sensitive to feedback inhibition by histidine but is instead subject to inhibition by aspartic acid. The other mutation, hisG3935, allows the histidine operon to be partially repressed by several amino acids, including aspartic acid. Analysis of hisG3935 is consistent with the hypothesis that phosphoribosyltransferase is directly involved in the regulation of the histidine operon.


Asymmetric distribution of guanine plus thymine between complementary strands of deoxyribonucleic acid of members of the Enterobacteriaceae. Analysis of the deoxyribonucleic acid prepared from Proteus mirabilis, Escherichia coli, and Serratia marcescens in an alkaline CsCl gradient has shown that there is an asymmetric distribution of guanine plus thymine residues between the complementary strands of the deoxyribonucleic acid.


Tryptophanase-positive bacteria in the marine environment. The prevalence of indole-positive organisms in the gut and environment of marine animals was studied. Indole formation by a group of the isolates was found to occur only in the presence of tryptophan. The isolates examined were all assigned to the genus Vibrio.


Salmonella locus affecting phosphoenolpyruvate synthase activity identified by a deletion analysis. Strain leu-4017, derived from Salmonella typhimurium LT2, cannot utilize acetate, pyruvate, or citric acid cycle intermediates as sole sources of carbon. The mutation in this strain extends from the A cistron of the leucine operon to some point between leu and azi, presumably deleting one or more loci involved in the utilization of these compounds. One of these loci is required for phosphoenolpyruvate synthase activity.


Autolytic activity associated with competent group H streptococci. Competent cells of group H streptococci strains Wicky and Challis autolyzed markedly when placed at 37 C in 0.05 m tris(hydroxymethyl)methyl-amino-propane sulfonic acid buffer (pH 9.0 to 9.1) containing 0.02 m 2-mercaptoethanol, whereas noncompetent cells autolyzed slightly. Autolysis of competent Wicky cells did not occur at 0 C or after the cells were heated at 100 C for 5 min. Culture fluids derived from strain Challis that contained competence factor (CF) activity did not contain lytic activity. Addition of native deoxyribonucleic acid (DNA) to competent Wicky cells caused a retardation in the rate of autolysis; ribonucleic acid and alkali-denatured DNA had less of an effect. Supernantant fluids derived from competent cell lysates lysed noncompetent Wicky cells but were inactive against cells of Hydrogenomonas eutropha, a group A Streptococcus, and against a commercial lysozyme substrate (Micrococcus lysodeikticus). This lytic activity was inactivated by heat (5 min at 100 C). Electron microscopic observations of autolyzed cells showed that autolysis occurs only at the site of cross-wall formation. A close relationship between the development of competence and autolysis is suggested by the fact that certain conditions that prevent the establishment of the competent state in Wicky populations (such as no CF, addition of CF simultaneously with chloramphenicol, and addition of trypsin-inactivated CF) also prevent autolysis. This observation emphasizes the indirect or inductive nature of CF on these processes.


Physical studies of the drug-resistance transfer factor in Proteus. The transfer unit, RTF, derived from an R factor has been transferred to Proteus mirabilis and characterized physiochemically.


Biosynthesis of the iron-transport compound enterochelin: mutants of Escherichia coli unable to synthesize 2,3-dihydroxybenzoate. Mutants of Escherichia coli K-12 blocked in each of the three enzymatic reactions between chorismate and 2,3-dihydroxybenzoate, in the pathway leading to the iron-sequestering compound enterochelin, have been isolated and biochemically characterized. The three genes concerned (designated entA, entB and entC) have been shown to be clustered on the chromosome between purE and gal and to be located near minute 14 by cotransduction with the purE, lip, and fep genes. entA, entB, and entC were shown to be the structural genes for 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase, 2,3-dihydro-2,3-dihydroxybenzoate synthetase, and isochorismate synthetase, respectively.


Metabolism of D-arabinose: a new pathway in Escherichia coli. Several growth characteristics of Escherichia coli K-12 suggest that growth on l-fucose results in the synthesis of all the enzymes necessary for growth on d-arabinose. Conversely, when a mutant of E. coli is grown on d-arabinose, all of the enzymes necessary for immediate growth on l-fucose are present. Three enzymes of the l-fucose pathway in E. coli, l-fucose isomerase, l-fuculokinase, and l-fuculose-l-phospháte aldolase possess activity on d-arabinose, d-ribulose, and d-ribulose-l-phosphate, respectively. The products of the aldolase, with d-ribulose-l-phosphate as substrate, are dihydroxyacetone phosphate and glycolaldehyde. l-Fucose, but not d-arabinose, is capable of inducing these activities in wild-type E. coli. In mutants capable of utilizing d-arabinose as sole source of carbon and energy, these activities are induced in the presence of d-arabinose and in the presence of l-fucose. Mutants unable to utilize l-fucose, selected from strains capable of growth on d-arabinose, are found to have lost the ability to grow on d-arabinose. Enzymatic analysis of cell-free extracts, prepared from cultures of these mutants, reveals that a deficiency in any of the l-fucose pathway enzymes results in the loss of ability to utilize d-arabinose. Thus, the pathway of d-arabinose catabolism in E. coli K-12 is believed to be: d-arabinose right harpoon over left harpoon d-ribulose --> d-ribulose-l-phosphate right harpoon over left harpoon dihydroxyacetone phosphate plus glycolaldehyde. Evidence is presented which suggests that the glycolaldehyde is further oxidized to glycolate.


Aspects of the mechanism of action of some cephalosporins. Cephaloridine and cephalexin had no effect on ribonucleic acid (RNA), deoxyribonucleic acid (DNA), or protein synthesis in Escherichia coli. However, cephalosporin 7/30 [7-(S-benzylthioacetamido)-cephem-3-ylmethyl-N -dimethyldithiocarbamate-4-carboxylic acid] and dimethyldithiocarbamate (DMDT), which occupies the side chain at position 3 in the 7/30 molecule, inhibited protein synthesis (and, to a lesser extent, RNA and DNA syntheses) in E. coli and had an inhibitory effect on the growth of Saccharomyces carlsbergensis. A bioautograph technique showed that two inhibitory spots were obtained with 7/30 but only one such spot with cephaloridine. Release of DMDT onto or in the bacterial cell may be responsible for "unusual" mode of action of cephalosporin 7/30.


A scanning electron microscope study of the lactating mammary gland.


The geometry of peripheral myelin sheaths during their formation and growth in rat sciatic nerves. In rat sciatic nerves, a small bundle of fibers was identified in which myelin sheaths were absent at birth, appeared within 3 days, and grew rapidly for 2 wk. During this interval, nerves were removed from littermates and were sectioned serially in the transverse plane. Alternating sets of thin and thick sections were used to prepare electron micrograph montages in which single myelinating axons could be identified and traced distally. During the formation of the first spiral turn, the mesaxon's length and configuration varied when it was studied at different levels in the same Schwann cell. The position of the mesaxon's termination shifted while its origin, at the Schwann cell surface, remained relatively constant. Along myelin internodes composed of two to six spiral turns, there were many variations in the number of lamellae and their contour. Near the mesaxon's origin, longitudinal strips of cytoplasm separated the myelin layers. Thicker sheaths were larger in circumference, more circular in transverse sections, and more uniform at different levels. Irregularities were confined to the paranodal region, and separation of lamellae by cytoplasm occurred at Schmidt-Lantermann clefts. Approximate dimensions of the bundle, its largest fibers, and their myelin sheaths were measured and calculated. The myelin membrane's transverse length and area increased exponentially with time; the growth rate increased rapidly during the formation of the first four to six spiral layers and remained relatively constant during the subsequent enlargement of the compact sheath.


Observations on organization of Z-disk components and on rod-bodies of Z-disk origin.


Percy Lavon Julian, Ph.D., Sc.D., LL.D., L.H.D., 1899- ?.


Some unsolved problems in paediatric neurosurgery.


Stereotaxic treatment of pain.

Stereotaxic treatment of pain.


Ten-year experience with a new method in the treatment of craniosynostosis.


Experimental and clinical evaluation of a British cryogenic lesion maker.


Five modes of subtraction using artificial slow flow carotid angiography.


The mesh skin graft in trauma: history and preliminary report on acute wound coverage.


Effects of 2-amino-4-hydroxy-6-carboxy-7,8-dihydropteridine and 2-amino-4-hydroxy-6-formyl-7,8-dihydropteridine on growth of Escherichia coli.


Overproduction of hydroxymethylpyrimidine by a thiamine regulatory mutant of Escherichia coli.


Back-door doctor. A study in private enterprise.


Fresh approaches to transplantation.

Fresh approaches to transplantation.


Revival.

Revival.


The changing face of "Gazette".

The changing face of "Gazette".


The last fifty years of medicine.

The last fifty years of medicine.


Medical school memories.

Medical school memories.


Forty years on in general practice.

Forty years on in general practice.


A study of intermittent positive pressure ventilation.


Forty years of medical art. A biography of Dorothy Davison.


The moment of death. An historical commentary.


National health insurance: what kind and how much. 2.


Depressions in childhood and adolescence. 2.


From the beginning.

From the beginning.


Dr Brock Chisholm. A tribute by the Director-General.


Salmonella immunity in mice.

Salmonella immunity in mice.


A study of staphylococci isolated from the upper respiratory tract of different animal species. IV. Physiological properties of Staphylococcus aureus strains of hare origin.


The virulence for mice of Salmonella typhimurium T1 mutants.


Histological and immunohistochemical studies on spontaneous renal lesions of Praomys (Mastomys) natalensis.


Gabritschewsky--the forgotten man of scarlet fever.


True childhood pemphigus. Pemphigus foliaceus in an 18-month-old child: immunofluorescence as a diagnostic aid.


A comparison of hycanthone, niridazole and placebos in the treatment of Schistosomiasis Mansoni.


Caries prevention in pits and fissures sealed with an adhesive resin polymerized by ultraviolet light: a two-year study of a single adhesive application.


Pit and fissure sealants.

Pit and fissure sealants.


Foley's footnotes. Toothache.

Foley's footnotes. Toothache.


Heart autotransplantation in dogs: a new technical approach.


Recollections of school days with Frank Ford.


Homotransplantations of "live" hearts.

Homotransplantations of "live" hearts.


Dr. Ford as seen by former students and house officers (Frank R. Ford).


Therapeutic approach for dissecting aneurysm.


Dr. Ford as he appeared to his colleagues (Frank R. Ford).


Inborn and acquired abnormalities of the complement system of man.


Speaking of Dr. Ford for the field of neurology (Frank R. Ford).


Frank Rodolph Ford. October 21, 1892-October 24, 1970.


Studies on the infection of Mycoplasma pneumoniae. II. Role of lipids of Micoplasma pneumoniae organisms.


Community nursing services accredited by NLN-April, 1971.


A review correlating the medical-dental relationship in the cranio-mandibular syndrome.


Airway obstruction and collateral ventilation.


Surgery in the elderly. 1.

Surgery in the elderly. 1.


Information, systems, and psychoanalysis. An evolutionary biological approach to psychoanalytic theory.


Imperative changes in detection methods for breast cancer.


Nuclear inclusions in subacute spongiform encephalopathy.


Computer analysis of the orthogonal electrocardiogram and vectorcardiogram in 1,002 patients with myocardial infarction.


Cardiovascular function in potassium-depleted dogs.


Alcoholic hepatitis. Natural history and evaluation of prednisolone therapy.


Malignant lymphoma of the spinal epidural space.


Infrared spectra of transfer ribonucleic acids. 3. Fragments of formyl-methionine tRNA from Escherichia coli.


A cross-over comparison of the effect of morphine, pethidine, pentazocine, and phenazocine on biliary pressure. The effects on biliary pressure of pentazocine, morphine, pethidine, and phenazocine were compared by direct measurement through a ;T' tube after choledochotomy. In two within-patient comparisons, the mean increases in biliary pressure following intramuscular morphine were significantly greater than those following pentazocine. When pethidine and phenazocine were compared with pentazocine, the mean increases were lower following pentazocine but not to a statistically significant extent. Pentazocine appears to be the most appropriate strong analgesic in biliary and pancreatic disease.


Alcohol and the liver.

Alcohol and the liver.


Gastrointestinal structure and function in germ-free or gnotobiotic animals.


In memoriam M Gen Amir Chand.

In memoriam M Gen Amir Chand.


Serological survey of incidence of ECHO 7 infection.


Free osseous tissue autografts as a predictable procedure.


Alpha-fluoro-and alpha-hydroxypyridylalanines.


Preservation in prosthodontics.

Preservation in prosthodontics.


Dimensions and displacement patterns of the posterior palatal seal.


Laboratory and clinical study on a permanent type base for transferring interocclusal records.


Fit of the removable partial denture base and its effect on abutment tooth movement.


A wash technique using rubber-base impression material.


A simplified technique for fitting cast restorations.


Prosthetic management of edentulous mandibulectomy patients. II. Clinical procedures.


Fabrication of a plastic facial moulage.


Controlled production of acute esophagitis. Experimental animal model.


Sequential gross, microscopic and roentgenographic features of acute feline esophagitis.


Footnote to medical history: Boerhaave, Brett, and Waterhouse. Author locates lecture notes of Boerhaave on tercentenary of his birth.


Anesthesia experience with living renal transplant donors.


Anesthesia in the Bible and Talmud.

Anesthesia in the Bible and Talmud.


Dermatologists in a changing world.

Dermatologists in a changing world.


Robert Thornton Patrick, M.D.

Robert Thornton Patrick, M.D.


Rapid weight reduction and psoriasis.

Rapid weight reduction and psoriasis.


Metastatic malignant melanoma of the tongue.


Studies in bullous diseases. Treatment of pemphigus vulgaris with immunosuppressives (steroids and methotrexate) and leucovorin calcium.


Dr. Harold William Bennetts, C.B.E., D.V.Sc.


Some oral lesions in the cat.

Some oral lesions in the cat.


Electron microscope studies of the effects of endo- and exopeptidase digestion on tropocollagen. A novel concept of the role of terminal regions in fibrillogenesis.


Iron requirements in infancy.

Iron requirements in infancy.


Is positive pressure breathing over-rated?


Clinical experience with a new discoid prosthesis for mitral valve replacement.


Ventricular septal defect and ventricular aneurysm following myocardial infarction.


Music and the modern generation.

Music and the modern generation.


Child welfare in ancient Rome.

Child welfare in ancient Rome.


Phosphorolysis of tRNA. Multiple conformational states of the tRNA in solution.


Phosphorolysis of the tRNA. Conformations of specific tRNAs and effect of the localized regions on the stability of the structure.


Binding sites of ribosomal proteins on two specific fragments derived from Escherichia coli 50 S ribosomes.


The somatotypes of athletes--a review.

The somatotypes of athletes--a review.


Microtubules in the heliozoan axopodium. 3. Degradation and reformation after dilute urea treatment.


Red-light stimulation of prolamellar body recrystallization and thylakoid formation in barley etioplasts.


A biological system producing a self-assembling cholesteric protein liquid crystal.


Effect of adrenergic-blocking or -stimulating agents on plasma growth hormone, immunoreactive insulin, and blood free fatty acid levels in man. In order to determine whether an adrenergic mechanism is involved in the secretion of growth hormone and insulin, the effect of adrenergic-blocking or -stimulating agents on plasma human growth hormone (HGH), immunoreactive insulin, blood free fatty acids (FFA), and glucose levels was studied in normal human subjects. The intravenous infusion of propranolol, a beta adrenergic-blocking agent, caused a rise in plasma HGH, a transient decrease in blood FFA, and no significant change in plasma insulin. This increase in plasma HGH was inhibited either by the combined administration of isoproterenol, a beta adrenergic-stimulating agent, along with propranolol or by oral glucose loading immediately before the start of propranolol infusion. The concomitant administration of epinephrine and propranolol brought about a rise in plasma HGH comparable with that produced by propranolol alone, without any significant change in blood FFA. Alpha adrenergic blockade by the intravenous infusion of phenotolamine significantly suppressed plasma HGH responses to insulin-induced hypoglycemia and to arginine infusion, and enhanced plasma insulin response to arginine infusion. It also stimulated lipid mobilization significantly. The intravenous infusion of alpha adrenergic-stimulating agents, phenylephrine and methoxamine, caused an increase in plasma HGH, a slight decrease in blood FFA, and no significant change in plasma insulin. This increase in plasma HGH was significantly inhibited by the simultaneous administration of phentolamine along with methoxamine. On the contrary, a beta adrenergic stimulant, isoproterenol, raised plasma insulin and blood FFA, and abolished the plasma HGH response to propranolol. Another beta stimulator, isoxsuprine, raised blood FFA but not plasma insulin. It is concluded that either beta adrenergic blockade or alpha stimulation enhances HGH secretion and inhibits insulin secretion and fat mobilization, whereas either alpha blockade or beta stimulation stimulates insulin secretion and fat mobilization and inhibits HGH secretion.


Development of a transplantation team in Indiana. I.


The effects of the morphine analogue levorphanol on leukocytes. Metabolic effects at rest and during phagocytosis. Studies on bacteria have suggested that morphine-like drugs have effects on the cell membrane. To determine the effect of this class of drugs on a mammalian cell, we selected the rabbit peritoneal exudate granulocyte, which undergoes striking membrane changes during phagocytosis. We examined the effect in vitro of the morphine analogue, levorphanol on phagocytosis and metabolism by granulocytes incubated with and without polystyrene particles or live Escherichia coli. Levorphanol (1 or 2 mmoles/liter) decreased: (a) acylation of lysolecithin or lysophosphatidylethanolamine in the medium (which is stimulated about two-fold during phagocytosis) both at rest (40%) and during phagocytosis (60%); (b) uptake of latex particles and Escherichia coli, as judged by electron microscopy; (c) killing of live Escherichia coli (10-fold); (d) (14)CO(2) production from glucose-1-(14)C during phagocytosis by at least 80%; (e) K(+) content of granulocytes (35%); (f) oxidation of linoleate-1-(14)C by 50%, and its incorporation into triglyceride by more than 80%. However, levorphanol stimulated 2 to 3-fold the incorporation of linoleate-1-(14)C or palmitate-1-(14)C into several phospholipids. Glucose uptake, lactate production, and adenosine triphosphate (ATP) content are not affected by the drug. Thus, levorphanol does not appear to exert its effects through generalized metabolic suppression. Removal of levorphanol by twice resuspending the granulocytes completely reverses all inhibition. In line with observations on bacteria, it appears that the complex effects of levorphanol on granulocytes may be due at least in part to an effect on the cell membrane.


Relative effects of pregnancy, estradiol, and progesterone on plasma insulin and pancreatic islet insulin secretion. Influences of estrogen and progesterone on the development of hyperinsulinemia and augmented pancreatic islet insulin secretion during pregnancy were assessed in this study. Groups of female rats were injected subcutaneously for 21 days with varying daily dosages of estradiol benzoate or progesterone in oil. On day 21, pancreatic islets were isolated by a collagenase method. Total insulin secretion was measured after 90-min incubations of 10 islets in buffered medium containing glucose. Higher physiologic dosages of estradiol or progesterone, singly or in combination, significantly increased islet secretion above values of untreated control rats and were comparable to augmented islet responses of term, 3-wk pregnant rats. Diameter and protein content of islets obtained from steroid-treated and pregnant rats exceeded control measurements in these instances. However, 2-hr preincubations of control islets with 1 or 10 mug/ml of either steroid did not influence subsequent glucose-stimulated insulin output. In related studies, plasma insulin responses during 30 min intravenous glucose tolerance tests were significantly above control responses in term-pregnant rats and animals receiving comparable dosages of steroids for 3 wk. Unlike pregnancy or progesterone treatment, estradiol administration alone or with progesterone significantly lowered postchallenge plasma glucose concentrations. These results indicate that estradiol and progesterone contribute to enhanced islet insulin secretion and plasma insulin responses to glucose administration during pregnancy. This change is not acutely produced but can be related to hypertrophy of islets following chronic hormonal administration. Although the data do not distinguish between direct and indirect beta-cytotrophic effects of these sex steroids, metabolic actions of estradiol and progesterone may differ, since estrogen treatment lowers plasma glucose curves following the induction of hyperinsulinemia.


A goodly heritage. An appreciation of the life and work of the late Dr Helen Mayo.


Autoantibodies in bullous pemphigoid.

Autoantibodies in bullous pemphigoid.


Resistant Plasmodium falciparum malaria cured by high quinine dosage.


On legal abortion.

On legal abortion.


John Alexander Campbell Bamford.

John Alexander Campbell Bamford.


Ellery Arthur Mulvihill Ryan.

Ellery Arthur Mulvihill Ryan.


Thomas James Biggs.

Thomas James Biggs.


Michael Balint.

Michael Balint.


Medicine in Ohio in the mid-nineteenth century. II.


Circadian variations in the effect of x-irradiation on the haematopoietic stem cells of mice.


The burned child.

The burned child.


Dermatophyte test medium. A diagnostic aid for practitioners.


Elimination of anemia in the newborn.

Elimination of anemia in the newborn.


The adaptation of microtechnic to platelet counts with the coulter counter.


Titers of antilymphocytic sera determined by immunofluorescence.


Premenstrual tension: symptoms and weight changes related to potassium therapy.


A simplified technique for evaluation of the biophysical properties of cervical mucus. A simplified technique for evaluating the biophysical properties of cervical mucus was presented.  The mucus was stretched on a microscope slide, and a cover slip was placed over a portion of the sample and dried for 24-48 hours at 37 degrees C.  Photomicrographs were taken under dark-field illumination.  Mucus samples representative of the normal midcycle (Day 12), proliferative phase (Day 8), secretory phase (Day 26), and midcycle from an infertile patient with diagnosis of hostile cervical mucus were examined.  The normal midcycle mucus showed linear alignment of dendritic crystals.  The proliferative sample showed linear alignment, but the dendritic crystals were sparse, widely spaced, and reduced in branching compared with the normal midcycle mucus.  Secretory mucus showed the dendritic crystals in a network pattern instead of in the linear alignment.  The abnormal (infertile) midcycle mucus showed evidence of alignment; however, the dendritic crystals were very wide and appeared obstructive.  These results indicate that there is a cyclic change in cervical mucus which is reflected in the pattern of the dendritic crystals formed.


Immune carrier state of feline panleukopenia virus-infected cats.


Quantitative aspects of the electrophoresis of RNA in polyacrylamide gels.


Blood-sucking behavior of terrestrial arthropods.


Edward Arthur Steinhaus, 1914-1969.

Edward Arthur Steinhaus, 1914-1969.


Microbial biosynthesis of B12-like compounds.


Saprophytic coryneform bacteria.

Saprophytic coryneform bacteria.


The regulation of phage development.

The regulation of phage development.


Effect of virus infections on the function of the immune system.


Characterization of ribonucleic acid transcribed in vitro on phage phi 80 deoxyribonucleic acid.


The interaction of cyclodextrins with nucleic acids. A study of secondary structure in three transfer ribonucleic acids.


Escherichia coli L-asparaginase. Catalytic activity and subunit nature.


Nucleotide clusters in deoxyribonucleic acids. Separation of oligo-nucleotides released by deoxyribonuclease I.


Presidential Address. The road to recognition.


Common sense in the treatment of pilonidal disease.


Ultrastructure and significance of microorganisms found in the formalin-fixed brain from a case of Parkinsonism.


Biochemical activity of coagulase positive and coagulase negative staphylococci isolated from pathological material.


Isolation of anaerobic bacteria from clinical material.


Intraribosomal environment of the nascent peptide chain.


Feeding in Rhodnius prolixus: mouthpart activity and salivation, and their correlation with chanees of electrical resistance.


Penetration of human fetal erythrocytes by Plasmodium falciparum in vitro.


Glomerulonephritis in Aleutian disease of mink: ultrastructural studies.


Save those teeth for the cleft palate patient.


John Wolcot (1738-1819).

John Wolcot (1738-1819).


Henry Norman Bethune.

Henry Norman Bethune.


Desiderius Erasmus. The spirit and the flesh.


The "Strange Case" of Robert Louis Stevenson.


The alcoholism of Eugene O'Neill.

The alcoholism of Eugene O'Neill.


Non-ferritin iron compound in rat small intestinal mucosa during iron absorption.


Reversible uremia secondary to renal replacement by lymphoma.


Evaluation of cirrhotic patient for surgery.


Distribution and pathogenicity of Tetrahymena limacis in the slug Deroceras reticulatum.


Haemochromatosis and diabetes.

Haemochromatosis and diabetes.


The telescopic system in the dental practice. Prosthetic therapy with telescoping dentures after elevation of the bite.


A method for more exact impressions of fixed partial dentures.


Changes in blood oxygen affinity and hemodynamics in anemic dogs.


The effects of dantrolene sodium on spasticity and motor performance in hemiplegia.


The long undescribed inverted clubfoot.

The long undescribed inverted clubfoot.


Recent advances in Fabry's disease.

Recent advances in Fabry's disease.


The role of humoral antibodies in the rejection of renal homografts by rabbits.


Colony-forming cells in the spleen. Determination of the fraction transplanted.


The role of tissue cells in anti-A hemagglutinin formation in rabbit skin allografts.


Prolongation of allograft survival with antimacrophage serum.


Chronic kidney allograft reactions in rats.


Effects of immunosuppressive treatment on rejection of heart allografts in rats.


Seventh report of the human kidney transplant registry.


Description of kidney transplant function during first year.


Proliferation of spleen-derived cells in thymus of lethally irradiated mice.


Effect of normal serum on survival of rat ovary allografts.


Studies on the suitability of blood-free media for the enumeration of clostridia.


Effect of digitalis on the left ventricular ejection time: a multicenter double-blind comparative study of digitoxin and pentaformylgitoxin in normal subjects.


In memoriam. Spencer Roane Atkinson (1886-1970).


A study of the dimensional changes occurring in the maxilla following closed face partial immediate denture treatment.


Personal identification from artificial dentures.


Intercalation of permeases during membrane growth.


Edward Jackson, M.A.,M.D.

Edward Jackson, M.A.,M.D.


Pastoral care program, as related to dialysis and transplantation.


Control of sulfur oxide emissions from primary copper, lead and zinc smelters--a critical review.


Renal transplantation evaluation and pre-operative care.


Psychiatric problems in renal dialysis and transplantation.


Directory of members of the Society for Personality Assessment as of April 1, 1971.


Oral pemphigus and bullous pemphigold. Immunofluorescent studies of two patients.


Two-staged cardiac replacement using an orthotopic prosthesis.


Contributions of an international heart registry.


Comments on pathogenesis of the rejection phenomenon.


Acute cardiac allograft rejection--morphological findings.


The role of platelets in chronic rejection.


Non cardiac autopsy findings after heart transplantation.


Microbiological aspects of human heart allograft recipients.


The role of soluble transplantation antigens in the induction of organ transplant tolerance.


Is a second cardiac transplant possible?


The Thackerays and medicine.

The Thackerays and medicine.


Velia and the school of Salerno.

Velia and the school of Salerno.


The origins of the sympathetic nervous system from Vesalius to Riolan.


Some historical aspects of leprosy in Ceylon during the Dutch period 1658-1796.


Some letters from Cardinal Newman to Dr. J. W. Ogle.


Did a dental profession exist in Ancient Egypt?


Relationship between transmembrane action potentials of single cardiac cells and their corresponding surface electrograms in vivo and in vitro, and related electromechanical phenomena.


Anti-inflammatory properties of medrysone in external ocular inflammations.


Sequelae of neonatal inclusion conjunctivitis and associated disease in parents.


Subscleral sclerectomy: a double-flap operation for glaucoma.


The Agnew-Verhoeff incision for acute phlegmonous dacryocystitis.


Regulation of disorders of the female cycle.


New aspects of the physiology of progestational agents.


Medicine in Australia today.

Medicine in Australia today.


Induction of ovulation with sexual steroids.


An immunofluorescent test for Toxoplasma gondii in tissues.


Lesions in Escherichia coli cell walls caused by the action of mouse complement.


The metabolism of different immunoglobulin classes in irradiated mice. V. Contribution of the gut to serum IgA levels in normal and irradiated mice.


Comparative analysis of the concentration of injected horseradish peroxidase in cytoplasmic granules of the kidney cortex, in the blood, urine, and liver. The concentration of horseradish peroxidase in total particulate fractions from the kidney cortex did not change much during the first few hours after injection, as long as most of the injected protein was not yet cleared from the blood. It decreased at a rate of 6-8% per hr afterwards. The concentration of peroxidase in total particulate fractions increased in proportion to the load (dose) over a wide range, suggesting that a constant fraction of the protein was reabsorbed by micropinocytic vesicles into the tubule cells from the glomerular filtrate. The amount of peroxidase excreted in the urine also increased in proportion to the injected dose. The proportion of peroxidase taken up by the liver, however, decreased several times when the dose was increased. A marked decrease of protein uptake into the kidney cortex and an increase of urinary excretion were observed when rats received a second, equal dose of peroxidase 4 hr after the first injection, and the rate of clearance of peroxidase from the blood was decreased after the second injection. The liver, on the other hand, took up almost twice as much peroxidase after two injections as after one. The uptake of peroxidase by the kidney cortex increased with age. Cytochemical observations on the preferential absorption of peroxidase by certain cell types and segments of the renal tubules in relation to dose are reported.


Results of tympanoplasty and mastoidectomy at the Massachusetts Eye and Ear Infirmary.


Lymphoma of the larynx.

Lymphoma of the larynx.


Directory of otolaryngologic societies.

Directory of otolaryngologic societies.


Twenty-four-hour perfusion and storage of canine kidneys with albumin and electrolyte solutions.


Characterization of proteins in urinary casts. Fluorescent-antibody identification of Tamm-Horsfall mucoprotein in matrix and serum proteins in granules.


Periodontal disease.

Periodontal disease.


Arterial blood gases during assisted ventilation in patients with chronic respiratory failure. I. Changes in the partial pressure of carbon dioxide (PaCO2).


Arterial blood gases during assisted ventilation in patients with chronic respiratory failure. II. Changes in the partial pressure of oxygen (PaO2).


Pesticides, pesticide residues, tolerances, and the law (U.S.A.).


Materials in contact with foodstuffs: technical and sanitary grounds in view of a general and specific legislation.


Hygienic normalization of pesticide residues and their tolerance levels in foodstuffs in the U.S.S.R.


Pesticide legislation and residue problems in Portugal.


Pesticide regulation in South Africa.

Pesticide regulation in South Africa.


Interaction between herbicides and soil microorganisms.


Changes in plasma growth hormone and insulin of the human foetus following hysterotomy.


Plasmodium falciparum gametocytes. Electron microscopic observations on material obtained by a new method.


Serological malaria survey in the Ethiopian highlands.


Blastoid transformation of lymphocytes in falciparum malaria.


Effect of nutritional repletion on human hookworm infection.


Follicular lymphoma of the spleen in patients with hepatosplenic Schistosomiasis mansoni.


Serological and bacteriological investigations of an outbreak of plague in an urban tree squirrel population.


Application of spectrophotofluorometric procedures to some problems in Mycobacterium leprae infections in mice and man treated with dapsone (DDS), diacetyl-DDS (DADDS), and di-formyl-DDS (DFD).


Relative susceptibility of acid-fast and non-acid-fast bacteria to ultraviolet light. Mycobacterium tuberculosis strain Erdman, M. bovis (BCG), and M. phlei showed a 1- to 2-log drop in viability after exposure to ultraviolet light compared to a 5-log drop over the same period for Staphylococcus albus, Listeria monocytogenes, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, and Serratia marcescens. L. monocytogenes showed an initial resistance to ultraviolet inactivation, but later the inactivation rate increased sharply. The significance of these findings with regard to the use of S. marcescens as a test organism for determining the bactericidal efficiency of ultraviolet lamps used to sterilize equipment contaminated with tubercle bacilli is discussed.


Characterization of Pseudomonas species isolated from clinical specimens. More than 90 morphological and physiological characters of 227 strains of pseudomonads isolated from clinical specimens and 16 reference strains are described. The clinical isolates included P. aeruginosa (apyocyanogenic), P. fluorescens, P. putida, P. pseudomallei, P. cepacia, P. acidovorans, P. alcaligenes, P. pseudoalcaligenes, P. stutzeri, P. putrefaciens, P. maltophilia, and P. diminuta.


Effect of water activity on enterotoxin B production and growth of Staphylococcus aureus. Staphylococcus aureus C-243, an enterotoxin B-producing strain, was cultured on media adjusted to various water activity (a(w)) levels by means of two different solute systems. Total numbers and rate of growth were diminished at low a(w) levels, and enterotoxin synthesis was extremely sensitive to reduction in a(w). A reduction of a(w) from 0.99 to 0.98 in one medium and from 0.99 to 0.97 in the other medium resulted in extremely low levels of enterotoxin in spent culture media despite the attainment of high numbers of staphylococci.


Survival of Salmonella anatum heated in various media. Survival of Salmonella anatum heated at 55 C for 35 min was determined in solutions of various chemical constituents of foods including salts, carbohydrates, amino acids, peptides, nucleic acids, gums, and stabilizers and compared with survival in 0.1 m phosphate buffer (pH 7.0). Commercially sterilized whole milk gave the most protection against heat. Trypticase Soy Broth, various peptide mixtures, and some amino acids gave substantial protection. Results with carbohydrates were variable, with mannitol, sucrose, and rhamnose providing substantial protection and glucose decreasing heat resistance. A few other substances, cysteine, glutathione, and sodium citrate, also decreased heat resistance. Pure proteins had little effect. Results show that water activity is of little significance under the test conditions. Protection from heat probably results from complexing of substances with heat-sensitive proteins in the cells. Autoclaved milk should not be considered equivalent to raw milk for studies of survival of bacteria during heating.


Incidence of infectious drug resistance among fecal coliforms isolated from raw sewage. Raw sewage was examined for the incidence of antibiotic-resistant coliforms present among both total and fecal coliforms. In both groups, it was found that approximately 3% of the coliform bacteria were resistant to two or more antibiotics. Of these organisms, 48% were capable of transferring all or part of their antibiotic resistance to an antibiotic-sensitive, F(-), derivative of Escherichia coli K-12. Among the R factors identified, those conferring resistance to streptomycin-tetracycline, ampicillin-streptomycin-tetracycline, and ampicillin or ampicillin-streptomycin accounted for 23, 20, and 15%, respectively, of the total R factors detected. The data indicate a significant level of infectious drug resistance among the fecal coliforms of the urban population. The data indicate further that because of the high incidence of coliform bacteria found to be doubly resistant to streptomycin and tetracyline, the inclusion of these antibiotics in selective media used for routine total or fecal coliform counts may serve to identify domestic sources of pollution.


Acute toxicity of ochratoxins A and B in chicks. Ochratoxins A and B were given to 1-day-old Babcock B-300 cockerels to evaluate acute toxic effects. Two trials with ochratoxin A gave 7-day oral median lethal dose estimates of 116 mug (3.3 mg/kg) and 135 mug (3.9 mg/kg) per chick. Chicks given daily oral doses of 100 mug of ochratoxin A died on the second day. Single subcutaneous doses of 400 mug of ochratoxin A were also lethal. The 7-day oral median lethal dose of B was estimated at 1,890 mug (54 mg/kg) per chick. Chicks given oral doses of 100 mug of ochratoxin B daily for 10 days survived. Sublethal doses of both ochratoxins A and B resulted in growth suppression which was proportional to the amount of ochratoxin given. Visceral gout was the principal gross finding. Microscopic examinations revealed acute nephrosis, hepatic degeneration or focal necrosis, and enteritis. Suppression of hematopoiesis in the bone marrow and depletion of lymphoid elements from the spleen and bursa of Fabricius were frequently seen. Both ochratoxins appeared to have similar pathological effects. This is the first report on the toxicity of ochratoxin B.


Production of bacterial cells from methane. A mixed methane-oxidizing bacterial culture capable of stable and predictable growth in continuous culture was isolated. The culture consisted of two types of gram-negative nonsporulating rods resembling pseudomonads. The culture grew well at 45 C on an inorganic medium without asepsis. Specific metal requirements for Ca(2+), Cu(2+), MoO(4) (2-), Zn(2+), Mn(2+), Mg(2+), and Fe(3+) (or Fe(2+)) were shown. The cells grown in continuous culture contained 11.7 to 12.1% total nitrogen. From an animal nutrition standpoint, the distribution of amino acids was satisfactory. The continuous fermentation was operated over a range of steady-state dilution rates from 0.085 to 0.301 hr(-1). The maximum specific growth rate for the culture, mu(max), was 0.303 hr(-1) (doubling time 2.29 hr). The average yield for all fermentations analyzed was 0.616 g (dry weight of cells per g of methane used and 0.215 g (dry weight) of cells per g of oxygen used. The yields on both methane and oxygen were higher for the oxygen-limited than for the methane-limited fermentations. The maximum productivity attained in the fermentor was 2.39 g (dry weight) of cells per hr per liter at a dilution rate of 0.187 hr(-1) and a cell concentration of 12.8 g (dry weight) of cells per liter. The limit on maximum cell productivity was determined only by the mass transfer rate of oxygen in the fermentor. The simultaneous volumetric mass-transfer coefficients (k(L)a in hr(-1)) for oxygen and methane were determined. The results appear to indicate an oxygen to methane mass-transfer coefficient ratio of approximately 1.4.


Factors affecting phosphate uptake by Aerobacter aerogenes in a system relating cell numbers to 32P uptake. The uptake of phosphate, from media limited in this ion, by resting cells of Aerobacter aerogenes has been investigated and shown to be dependent upon several factors. An incubation medium composed of 10(-2)m K(+), 5 x 10(-3)m Mg(2+), 1 mg of glucose per ml, and 1 muCi of (32)PO(4) (3-) per ml, buffered at pH 6.55 with 0.05 mN-2-hydroxyethyl-piperazine-N'-2-ethanesulfonic acid (HEPES), was found to stimulate optimum accumulation of (32)P-orthophosphate. The temperature of incubation, incubation time, the concentration of unlabeled orthophosphate, as well as arsenate, and several metabolic inhibitors were found to affect the accumulation. The labeled cells were collected on a membrane filter, which had been previously boiled in glass-distilled water, for measurement of the radioactivity accumulated. Under optimum conditions, as few as 20,000 cells were capable of accumulating detectable amounts of (32)P-orthophosphate in 1 hr of incubation.


Classification of Streptomyces spore surfaces into five groups. Streptomyces spores surfaces have been classified into five groups, smooth, warty, spiny, hairy, and rugose, by examination of carbon replicas of spores with the transmission electron microscope and by direct examination of spores with the scanning electron microscope.


Prevalence of Clostridium botulinum in semipreserved meat products. Samples of semipreserved meat products were taken, and 372 were examined for Clostridium botulinum. Five samples contained type A and one sample type B.


Apparatus for metabolic studies with anaerobes. An apparatus is described which allows metabolic experiments with obligate anaerobic bacteria to be performed with minimal disturbance of the E(h) of the culture.


Bacterial flora of the jejunum following peptic ulcer surgery.


Virus multiplication in culture cells synchronized by excess thymidine treatment. I. Effect of excess thymidine upon growth of poxviruses and chikungunya virus.


Management of recurrent hiatal hernia.

Management of recurrent hiatal hernia.


Absence of amino acid hydroxamate formation in aminoacyl transfer RNA formation stimulated by spermine.


Specificity in the association of histones with deoxyribonucleic acid. Evidence from derivative thermal denaturation profiles.


Peptidoglutaminase. Enzymes for selective deamidation of gamma-amide of peptide-bound glutamine.


Isotopic effects and inhibition of polysaccharide phosphorylase by 1,5-gluconolactone. Relationship to the catalytic mechanism.


Interaction of vernamycin A with Escherichia coli ribosomes.


Investigation of verapamil in the treatment of angina pectoris.


Effect of infection with R23 on DNA and RNA synthesis in Escherichia coli.


The enhancement of X-ray-induced DNA degradation in Micrococcus radiodurans by phenethyl alcohol.


The isolation of Vibrio parahaemolyticus and related halophilic bacteria from Canadian Atlantic shellfish.


Increased activity of soil microorganisms near sclerotia of Sclerotium rolfsii in soil.


Enteric bacterial metabolism of stream sediment eluates.


Thymidine diphosphate 4-acetamido-4,6-dideoxyhexoses. IV. Purification and properties of thymidine diphosphate 4-keto-6-deoxy-D-glucose transaminase from Pasteurella pseudotuberculosis.


Sequence of bovine liver glutamate dehydrogenase. II. Sequences of tryptic peptides.


The role of exonuclease and beta protein of phage lambda in genetic recombination. II. Substrate specificity and the mode of action of lambda exonuclease.


The sialic acids. XII. Synthesis of colominic acid by a sialyltransferase from Escherichia coli K-235.


A temperature-sensitive thy mutant blocked in the synthesis of thymidylate synthetase.


Enzymatic removal and replacement of nucleotides at single strand breaks in deoxyribonucleic acid.


Strand displacement during deoxyribonucleic acid synthesis at single strand breaks.


Medical resources and medical education: a student survey of physicians.


The polymorphic relationship of Cooperia oncophora (Railliet, 1898) Ransom, 1907, to Cooperia surnabada Antipin, 1931 (Nematoda: Trichostrongylidae).


Limitation of the lymphocyte cytotoxicity crossmatch test in recipients of kidney transplants having preformed antileukocyte antibodies.


Ralph Nader, the consumer and the hospitals.


Clinical use of rabbit antihuman lymphocyte globulin in cadaver-kidney transplantation.


Shattuck lecture--the physician and organized medicine.


Premature puff for smoking beagles.

Premature puff for smoking beagles.


Mechanism of DNA replication.

Mechanism of DNA replication.


Ball lightning and after-images.

Ball lightning and after-images.


After images and ball lightning.

After images and ball lightning.


Ball lightning or spots before the eyes?


Calcium shift into bone causing transient hypocalcaemia after injection of parathyroid hormone.


Mean red cell age as a determinant of blood oxygen affinity.


Effect of lithium ions on the release of acetylcholine from the cerebral cortex.


Observed and predicted 90Sr and 137Cs levels in milk.


Radiation-induced light flashes observed by human subjects in fast neutron, x-ray and positive pion beams.


Preliminary observations on tickling oneself.


H. J. Thomasson.

H. J. Thomasson.


Myocardial function from cineangiocardiograms with a digital computer.


Pulmonary perfusion changes after experimental unilateral bronchial occlusion and their clinical implications.


Philip Matthew Platten, M.D. 1928-1970.

Philip Matthew Platten, M.D. 1928-1970.


Jacob Gershon-Cohen, M.D., Sc. (Med.) 1899-1971.


Roger Keith Wallace, M.D. 1922-1970.

Roger Keith Wallace, M.D. 1922-1970.


A causerie on circumcision, congenital and acquired.


The use of proteolytic enzymes (chymoral) in sporting injuries.


A controlled trial of glifanan and omnopon in postoperative pain.


Some physiological effects of a mainly fruit diet in man.


Our secretary retires.

Our secretary retires.


A double-blind trial in hypertension comparing Baycaron (FBA 1500), hydrochlorothiazide and placebo.


Cholesterol, coronary heart disease and oestrogens.


The weight gain effect of periactin in anorexic patients.


Antinuclear factors. Their determination by immunofluorescence.


Prescription drug insurance for the elderly under Medicare.


The nature of uranium occurrence in the leaves of Coprosma australis (A. Rich.) Robinson.


Studies on nucleic acids of living fossils. II. Transfer RNA from the brachiopod lingula.


The small molecular weight monodisperse nuclear RNA's in mitotic cells.


Occurrence of cysteinyl transfer ribonucleic acid with a blocked alpha-amino group in rabbit reticulocytes.


Control of stable-RNA synthesis and maturation of 5-S RNA in Salmonella typhimurium RC-str and RC-rel.


Translational regulation of T4 messenger RNA metabolism.


Biogenesis of 30-S subunits in exponentially growing Escherichia coli.


Interaction of bacterial ribosomes with bentonite.


Messenger RNA accumulation in Escherichia coli during chloramphen- icol treatment.


The preparation of soluble DNA methylase from normal and regenerating rat liver.


A cryomicroscope for the study of freezing and thawing processes in biological cells.


Effect of 5-fluorouracil posttreatment on sensitivity to ultraviolet radiation and course of messenger RNA synthesis in Escherichia coli cells.


Division of Escherichia coli 15 TAU cells synchronized by arginine and uracil starvation.


A study of the afferent and efferent limbs of the immune response in opossums.


Immune response to hemocyanin in normal, thymus-deprived and reconstituted mice.


Genetic and cellular factors in the immune response. 3. The cellular response to poly(Glu52Lys33Tyr15) in the highly responding ACI and poorly responding F344 strains of inbred rats.


Attempts to abolish tolerance of skin homografts in mice with x-irradiated plasma from specifically sensitized donors.


Studies on cellular immunity to Salmonella typhi in vitro.


Lysis and death of Mycoplasma pneumoniae by antibody and complement.


Peyer's patches: lack of specific antibody-containing cells after oral and parenteral immunization.


Independent action of thymus and bone marrow cells during the secondary response of direct plaque-forming cells.


The mechanism of antibody-induced stimulation and inhibition of the immune response.


Molecular immunogenicity of bacterial lipopolysaccharide antigens: establishing a quantitative system.


Toxicity of seawater to coliform bacteria.


Refractive keratoplasty.

Refractive keratoplasty.


The induction of vascular renal allograft rejection by leukocyte sensitizatin.


Ampicillin in acute infections of general practice. A controlled trial.


George Bell.

George Bell.


William John Saxton.

William John Saxton.


Lloyd Edgar Buley.

Lloyd Edgar Buley.


Alexander Leslie Anderson.

Alexander Leslie Anderson.


Sarah Amanda Luse 1918-1970.

Sarah Amanda Luse 1918-1970.


Distribution of hyaluronidase in bovine urogenital organs: on immunofluorescent study.


Ultrastructure of the interdental cells in mammals.


Morphological variations of a hypha-forming strain of Cryptococcus neoformans (Coward strain) in tissues of mice.


Histopathological observations in experimental cryptococcosis caused by a hypha-producing strain of Cryptococcus neoformans (Coward strain) in mice.


Treatment of paralytic ileus with adrenergic neuronal blocking drugs.


Thomas Blizard Curling--the man and the ulcer.


The development of hypertension after canine renal preservation.


Sulfation factor regulation of skeletal growth. A stable mechanism dependent on intermittent growth hormone secretion.


Multiple skeletal complications in a case of chronic renal failure treated by kidney homotransplantation.


Nocardia asteroides infection complicating neoplastic disease.


Important factors in extracorporeal organ support with special reference to the heart.


Some observations on the three-dimensional growth of L5178Y cell colonies in soft agar culture.


Appearance of polyribosomes during germination of spores in cellular slime mold Dictyostelium purpureum.


Immunological studies in ulcerative colitis. V. Family studies.


Tisstue culture of adult human liver.

Tisstue culture of adult human liver.


The digestion and absorption of dietary folate.


Should anemia stop surgery?

Should anemia stop surgery?


The metal head cap. A delayed aid to the tagliacotian operation.


The use of Hydron in resection of the liver.


Antigen-binding cells in mice immune or tolerant to Escherichia coli polysaccharide. The number of PFC and of RFC was studied in mice which were unimmunized, immunized, or tolerant against lipopolysaccharide of E. coli 055:B5 origin. The number of PFC/10(6) spleen cells increased from 0.5 in normal to 209 in immunized mice. The corresponding figures for RFC were 93 and 513 RFC/10(6) spleen cells. In tolerant animals, which contained few or no PFC, the number of RFC was increased as compared to that found in unimmunized mice. The formation of rosettes was specific, since their formation was inhibited by soluble coli polysaccharide and by rabbit antisera against mouse immunoglobulins. The antigen-binding cells were not derived from thymus, neither in immune or tolerant mice, because they did not carry the theta antigen. It is suggested that the majority of antigen-binding cells present in tolerant animals are cells having receptors for the antigen of rather low affinity. The relevance of these findings for the induction of high and low zone tolerance is discussed.


Thymus-marrow immunocompetence. V. Hydrocortisone-resistant cells and processes in the hemolytic antibody response of mice. Corticosteroids suppress the humoral antibody response of mice to sheep erythrocytes. This response depends on interactions between thymus-derived helper cells and bone marrow-derived antibody-forming cell precursors (AFC precursors). Previous experiments had shown that spleen cells (a mixture of thymus-derived and marrow-derived cells) were sensitive to corticosteroids while AFC precursors in the bone marrow were resistant. The present experiments showed that the thymus of a mouse given 2.5 mg of hydrocortisone acetate, although containing only about 5% of the number of cells of a normal thymus, was as effective as a normal thymus in cooperating with bone marrow when transferred to irradiated syngeneic mice and stimulated with SRBC. The proliferative response of thymus helper cells to SRBC was also resistant to hydrocortisone. In this context, the majority of thymic cells are in the cortex, are rapidly dividing, are sensitive to corticosteroids and are not iminunocompetent. A small number of thymic cells, probably located in the medulla, are resistant to corticosteroids, but are immunocompetent since they can serve as helper cells. The hydrocortisone-sensitive phase of the splenic response to SRBC was found to be the bone marrow-derived AFC precursor since spleens from hydrocortisone-treated donors had immunocompetence restored by normal bone marrow but not by normal thymus cells.


A cellular deficit in the reconstitutive capacity of immune populations of lymphoid cells demonstrable in studies of delayed hypersensitivity in mice. Evidence for thymus-bone marrow cell synergism. A cell-transfer system was employed in the present work to investigate several characteristics of the capacity of immune and normal lymphoid cells to transfer the delayed response to methylated human serum albumin in lethally irradiated syngeneic recipients. Spleen cells derived from donor mice immunized with goose erythrocytes were far less effective in transferring responsiveness when compared with equal numbers of normal cells. Statistical analyses indicated a frequency of 1 reactive cell or cell unit in 1.3 x 10(7) normal cells and in 6.2 x 10(7) immune cells. These findings provided confirmatory evidence that antigen-induced suppression (antigenic competition) employing sequential administration of two non-cross-reacting antigens is due to relative deficits of immunocompetent cells generated by lymphoproliferation in lymphoid tissues secondary to immunization with the initial antigen. The cellular deficit in the immune population was shown to be resident in a thymus cell population, which restored the number of responders to a level equivalent to the normal population. The thymic cell was akin to the antigen-reactive cell. The cell limiting the degree of response, that is the effector cell for both normal and immune cell populations, was of bone marrow origin. Both populations of cells were shown to act in synergy to reconstitute the delayed response to the antigen.


Purification and physical properties of group C streptococcal phage-associated lysin. A purification procedure for the Group C phage-associated lysin is described utilizing tetrathionate to protect the enzyme's -SH group(s) from thiol-inactivating agents. A 652-fold purification has been accomplished yielding a solution in which the enzyme activity corresponds to essentially a single band on polyacrylamide gel which accounts for 70% of the total protein in the preparation. A molecular weight of 101,000 and frictional ratio of 1.526 was determined for the lysin utilizing experimentally determined values for its Stokes radius and sedimentation coefficient.


The sensitization of rats by allografts transplanted to alymphatic pedicles of skin. Pedicles of skin which lacked a lymphatic drainage were raised on the backs of rats in order to study the importance of afferent lymphatics in sensitization by skin allografts. Although allografts transplanted to the alymphatic pedicles enjoyed a prolonged survival, they contracted progressively from about 3 wk after transplantation and were reduced eventually to small scars. In contrast, autografts survived unchanged in size for the life-span of the pedicles which carried them. The slow contracture of the allografts was associated with sensitization of the host because test allografts applied orthotopically were destroyed with a second-set tempo. No regeneration of lymphatics from the long-standing pedicles could be demonstrated, and it was concluded that sensitization had occurred eventually through the blood, presumably by the process of peripheral sensitization. Allografts on skin pedicles could be destroyed rapidly by active or adoptive immunization, so it is probable that the level of sensitization to which they themselves gave rise was a low one. Although it is not disputed that afferent lymphatics are essential for the rapid destruction of skin allografts, it is clear that the absence of a lymphatic supply does not permanently exempt them from immunological attack in the rat.


Dr. Lubomír Syrůcek quinquagenerian.

Dr. Lubomír Syrůcek quinquagenerian.


Antigen-specific cells in mouse bone marrow. II. Fluctuation of the number and potential of immunocyte precursors after immunization. Quantitative and qualitative changes of mouse bone marrow cells were studied by limiting dilution assays 2-3.5 months after immunization of donors with sheep erythrocytes or unrelated antigens (Salmonella typhimurium, horse and chicken erythrocytes). Irradiated (C3H x C57BL/10)F(1) mice were reconstituted with an excess of nonprimed thymocytes and small graded numbers of primed bone marrow cells. Direct and indirect plaque-forming cells (PFC) were induced by secondary stimulation with SRBC and enumerated on the 9th day after cell transplantation. Marrow precursors of PFC (P-PFC) cooperated with thymocytes in the production of direct and indirect PFC after SRBC priming. The limiting dilution plots, which were not compatible with predictions of the Poisson model before immunization, changed and conformed to this model afterwards, as if the population of P-PFC had become functionally more homogeneous. The concentration of marrow P-PFC increased up to the 3rd month after priming, and decreased during the 4th, varying over two logarithms of nucleated marrow cells. The fluctuation was simultaneous and of the same order of magnitude for precursors of direct and indirect PFC, which were class restricted. A third effect of immunization was detected at 3.5 months: individual precursor units generated 3-4 times more direct and indirect PFC than at earlier intervals. Qualitative and quantitative changes of marrow P-PFC participating in anti-sheep responses were specific, since antigens unrelated to SRBC failed to induce them. The data suggested that marrow-derived cells were the major carriers of immunologic memory, but that they functioned in cooperation with thymus-derived inducer cells during secondary anti-sheep responses.


The use of fluorescent antibodies in the identification of streptococci. IV. Determination of group C streptococci.


Some regularities of antibody formation in mice immunized by salmonella cell fractions.


The central cancer registry, Melbourne, 1940-1970.


Fothering the ship.

Fothering the ship.


Alexander Leslie Anderson.

Alexander Leslie Anderson.


The growth of logical thinking. Piaget's contribution to ego psychology.


Disease of the colon.

Disease of the colon.


The surgery of peptic ulcer.

The surgery of peptic ulcer.


Identificaion and reidentification: a change in the self-concept.


A longitudinal study on children of endogenous psychotics.


Shock in Bantu siderosis.

Shock in Bantu siderosis.


Erroneous values on the Model S Coulter Counter due to high titer cold autoagglutinins.


In memoriam: Howard Freedman, M.D., 1933-1970.


Quantitative studies of localized leukocyte mobilization in acute leukemia.


A ribosomal-bound aminopeptidase in Escherichia coli B: substrate specificity.


Studies on the protein moiety of endotoxin from gram-negative bacteria. Characterization of the protein moiety isolated by phenol treatment of endotoxin from Serratia marcescens 08 and Escherichia coli 0 141:K85(B).


The synthesis of the alternating copolymer poly(r(A-s4U)) by RNA polymerase of Escherichia coli.


Fatty acid degradation in Escherichia coli. An inducible system for the uptake of fatty acids and further characterization of old mutants.


Effect of cholecystokinin and caerulein on gastric secretion in cats.


Tulane's training program for health facility surveyors.


A multidisciplinary approach to services for the epileptic.


The structure of bovine proinsulin.

The structure of bovine proinsulin.


Studies on the uptake of hexose phosphates. I. 2-Deoxyglucose and 2-deoxyglucose 6-phosphate.


Studies on the uptake of hexose phosphates. II. The induction of the glucose 6-phosphate transport system by exogenous but not by endogenously formed glucose 6-phosphate.


Studies of the mechanism of anthranilate synthase. Effect of hydroxylamine and N-methylhydroxylamine.


Current publication in gerontology and geriatrics.


Magnesium transport in Escherichia coli. Inhibition by cobaltous ion.


Purification of two valine transfer ribonucleic acid species from Escherichia coli and their coding properties.


Aspartate transcarbamylase. The use of primary kinetic and solvent deuterium isotope effects to delineate some aspects of the mechanism.


The changing pattern of the clinical apprenticeship. (Frederick Price Lecture, Trinity College, Dublin, June, 1970).


Comparison of left ventricular volumes by single-plane cineangiography and by indicator dilution.


Automated platelet counting with the autoanalyzer.


The antibacterial effect of normal and infected urinary bladder.


Laboratory identification of intravascular coagulation. The serial dilution protamine sulfate test for the detection of fibrin monomer and fibrin degradation products.


Measurement of extracellular fluid volume with 169Yb-diethylenetriaminepentaacetate.


David Lander Reeves, December 31, 1904--August 18, 1970.


Doctor Norman Clarke's fabulous collection of Michigan history.


Radiologic evaluation of the lung transplant.


Rat renal transplantation and its immunological study.


Induction of immunological tolerance by a hapten (DNP) bound to a non-immunogenic protein carrier.


Reactivities of shark 19S and 7S IgM antibodies to Salmonella typhimurium.


Plasmid specificity of two proteins required for conjugation in E. coli K12.


Publicly funded podiatry: the New York City Medicaid experience.


On the death of Dr. and Mrs. Richard Weinerman.


Synergistic immunodepressive effect of 7,12-dimethylbenz(a)anthracene and urethan in the rat.


Gut lactase activity: decrease during Salmonella typhimurium infection in mice.


Activity of tetracyclines, nalidixic acids, and nitrofurantoin in two experimental models of Escherichia coli urinary tract disease in rats.


Induction of interferon in human subjects by poly I:C.


In memoriam. John Bright Hopkins (1900-1970).


Weber on legitimate norms and authority.


A pneumotaxic centre in avian brain.

A pneumotaxic centre in avian brain.


Lymphflow and biochemical composition during homograft rejection.


Thyroid hormones and the gut.

Thyroid hormones and the gut.


When the heart stops.

When the heart stops.


Utilization review guidelines for home health agencies.


Transplantation of the heart.

Transplantation of the heart.


Space-age medicine.

Space-age medicine.


Renal transplantation.

Renal transplantation.


Problems of the spina bifida patient.

Problems of the spina bifida patient.


A diagnostic problem in the elderly patient.


Amantadine in Parkinsonism. An extended prospective trial.


Lactation inhibition in the Outer Hebrides. A trial of a fat-stored oestrogen.


Inhibition of lactation.

Inhibition of lactation.


Observations on the use of a vaporizer lamp in general practice.


Borotannic complex in athlete's foot.

Borotannic complex in athlete's foot.


George Sherman Ripley Jr., M.D., F.A.C.R. 1917-1970.


Paul O. Snoke, M.D. 1898-1970.

Paul O. Snoke, M.D. 1898-1970.


Issues and orientations in the evaluation of rehabilitation programs. 1. A review article.


Trichinella spiralis: intracellular spheroid bodies in "migratory" larva.


Effect on ascites of thoracic duct fistula and of cervical lymphatic-venous shunt.


Circulatory regulation after experimental tricuspid lesion. Haemodynamics during the development of congestive heart failure.


Segmental flexures of the notochord: artefact or not?


On the occurrence of lymphatic vessels in the adrenal gland of the white rat.


Extensive tracheal necrosis associated with a prestretched tracheostomy tube cuff.


The choroid plexus: a historical review.


Therapy of amebiasis.

Therapy of amebiasis.


Ambroise Pare, The Countess Margaret, multiple births, and hydatidiform mole.


Studies on muscle differentiation. 3. Immuno-histochemical identification of tropomyosin in skeletal and cardiac muscles of developing chick embryo.


The continuity of care coordinator.

The continuity of care coordinator.


5000 years of orthopaedics in India.

5000 years of orthopaedics in India.


An orthopaedic view of the treatment of fractures.


Immunofluorescent staining of the gastric mucosal glycoprotein in fetal gut.


History and organization of the traditional orthopaedic residency.


Mitral valve replacement by calf aortic heterograft and its clinical evaluation.


The effects of social legislation on the graduate teaching of orthopaedic surgery.


The development of motor skills in orthopaedic education.


The emotions and the ergotropic and trophotropic systems.


Response of asthmatics on three weeks Intal treatment.


Bronchopulmonary dysplasia in the premature baby.


Keratomycosis. Medical and surgical treatment.


Suppression of corneal graft reaction by antilymphocyte serum. 3. Effect of pretreatment of donor animals.


Altered vascular permeability in ocular inflammatory disease.


The lens.

The lens.


The vitreous.

The vitreous.


Studies in the movement of isotopes RI-131SA and Na24 from ventricular CSF fluid.


X-linked mental retardation without physical abnormality: Renpenning's syndrome.


Cognitive deficits from bilateral cingulotomy for intractable pain in man.


Clinical and electroencephalographic effects of cinanserin in schizophrenic and manic patients.


Golgi potassium-dichromate silver-nitrate impregnation. Nature of the precipitate studied by x-ray powder diffraction methods.


The influence of alcohol and marihuana on motor and mental performance.


The genus Paratriatoma in western North America.


Microspectrofluorimetric identification of m-hydroxyphenylethylamines (m-tyramines) in central and peripheral monoamine neurons.


Predictors of chlordiazepoxide response in anxiety.


Isolation of immunological adjuvant component from B.C.G. cells.


Histology of the principal islets of a fresh water teleost, Channa punctatus (Bloch).


Synaptic morphology in the spinal cord of normal and tricresylphosphate (TCP) poisoned hen. An electron microscopic study.


Electron microscopic observations of rat medullary reticular tissue after short-term, whole body gamma irradiation.


Toxins from fish and other marine organisms.


The flat sour bacteria.

The flat sour bacteria.


Food processing with added ascorbic acid.


Microbial sources of protein.

Microbial sources of protein.


Intermolecular cross-linking of collagen in human and guinea pig scar tissue.


Characterisation of long-acting thyroid stimulator gamma-G binding protein.


The purification and characterisation of chorismate mutase-prephenate dehydrogenase from Escherichia coli K12.


Characterisation of the subunits of chorismate mutase-prephenate dehydrogenase from Escherichia coli K12.


Crohn's disease.

Crohn's disease.


The use of nitrimidazine in the treatment of acute ulcerative gingivitis. A double-blind controlled trial.


Isolation of isoinhibitors of trypsin from porcine colostrum.


Clinical trial comparing pentazocine with pethidine and morphine in severe ischaemic limb pain.


The Schoemaker-Billroth II operation for gastric ulcer.


Drug treatment of rheumatic disorders in general practice. A comparative study of aspirin and an alkali-phenylbutazone preparation.


A comparison of guanoclor and methyldopa in the treatment of severe hypertension.


André Jacques, M.D., F.R.C.P.(C).

André Jacques, M.D., F.R.C.P.(C).


Experimental ocular toxoplasmosis in mice: immunofluorescent demonstration of toxoplasma antigens in the early stage of infection.


Effects of hepatectomy on the disappearance rate of lidocaine from blood in man and dog.


Isokapnic ventilation during surgical operations: description of equipment and first results.


Leroy W. Krumperman, M.D.

Leroy W. Krumperman, M.D.


Harderoporphyrin: a new porphyrin from the Harderian glands of the rat.


Attachment behaviour, object relations and the dynamic-economic points of view. Critical review of Bowlby's Attachment and loss.


A pagination converter relating the Gesammelte Werke to the Standard Edition of the complete psychological works of SigMund Freud.


Dentistry and politics--the Perpich brothers practice both.


Clinical evaluation of two types of resilient liners on dentures.


The influence of phosphoric acid etching on retention of acrylic resin to bovine enamel.


The individuation process as reflected by 'the Rosary of the Philosophers' (1550).


Origin of an "official organ". The Journal of the Oklahoma State Medical Association.


Transformation of fibroblasts by allogeneic and xenogeneic transplants of demineralized tooth and bone. Xenogeneic transplants of powdered, dehydrated, demineralized matrix of bone and tooth were well tolerated in three species of rodents. Differences between the species were found in competence of fibroblasts to be transformed into cartilage and bone in vivo by these preparations. Rat fibroblasts were most susceptible to transformation of this sort; they were transformed by demineralized dentin of guinea pig, mouse, and rat, and to a limited extent, by a specimen of decalcified human bone.


Cherokee history to 1840: a medical view.


Health and medical practice in the Choctaw Nation, 1880-1907.


A rapid slide method for urine cell counts.


The gradient plate for the preliminary determination of concentration of inhibitory and nutrient agents.


A robust and efficient slide holder for use with the cryostat.


Coagulase and deoxyribonuclease tests for staphylococci.


Bronchial lavage in asthma.

Bronchial lavage in asthma.


The problems of atherosclerosis.

The problems of atherosclerosis.


Clinical aspects of aneurysms and their management.


A spironolactone-hydroflumethiazide combination in hypertension.


The gout doctor. George Cheyne, M.D. (1671-1743).


The effect of acrolein on L-asparaginase 2 from Escherichia coli.


Action of actinomycin D on the GI tract: implications for enzyme induction studies.


Prevention of aminoglutethimide phosphate (AGP) block of ovulation in PMS-treated immature rats.


Effect of hypoxia and hypercapnia alone and in combination upon the circulating red cell volume of rats.


In vitro lymphocyte response to autologous cultured lymphoid cells.


Differential stimulation of mouse lymphoid cells by phytohemagglutinin and pokeweed mitogen.


Endotoxin-induced insulin sensitivity in BCG infected mice.


Immune competence of germ-free rats. I. Increased responsiveness to transplantation and other antigens.


Internal arteriovenous fistula for hemodialysis.