A double isotope method to measure vitamin B 12 absorption.


Berger rhythms and psychiatry.

Berger rhythms and psychiatry.


In memoriam Johannes Magaard Nielsen, 1890-1969.


Differential drug effects in neurotic depression.


Preparation of undecalcified bone sections.


Nitrogen balance studies in infants fed infant food based on groundnut flour.


Typhoid fever in children. Clinical and bacteriological studies.


Hemophilia.

Hemophilia.


Maternal factors in relation to birth weight.


Acrodermatitis enteropathica.

Acrodermatitis enteropathica.


Electrocardiographic study in the newborn.


Renal homotransplants in dogs.

Renal homotransplants in dogs.


In memoriam. Dr. A. C. Ukil.

In memoriam. Dr. A. C. Ukil.


Radiologic seminar C: roentgen diagnosis of anencephaly in utero.


Iatrogenic lumbar meningocoele: report of three cases. We have reported three cases of iatrogenic lumbar meningocoeles after surgery for herniated lumbar intervertebral discs. We reject the term spurious, pseudo, or false as given by earlier writers. We feel that, in reality, these sacs are true meningocoeles, with complete arachnoidal lining and freely communicating with the intraspinal subarachnoid space. We have also given a brief outline of the clinical features, elucidated the mechanisms operative in the production of symptoms, and suggested the cardinal features of radiographic diagnosis. Prevention is, of course, the best way to avoid this complication. If a dural tear does occur, every effort should be made to suture it in a watertight manner.


A new agent for the control of spasticity. In a preliminary controlled trial, CIBA 34,647-Ba, a gamma aminobutyric acid derivative, was found to be more effective than placebo in reducing spasticity due to spinal injuries. In an uncontrolled trial, 34,647-Ba also appeared more effective than diazepam. The intensity of spasticity was measured electromyographically by the amplitude of the stretch reflex at various velocities, and the results were correlated with those obtained by clinical assessment. 34,647-Ba was effective in both complete and incomplete spinal cord lesions and it is suggested that it has an action at the spinal level. No significant side-effects were encountered.


Internal arteriovenous fistulae for dialysis in children.


Neurosurgery in cerebral palsy.

Neurosurgery in cerebral palsy. Stereotaxic subthalamotomy of 55 patients with cerebral palsy gave a good result in 65% of the selected cases. The result was uncertain in 15 and poor in 20%. An independent socio-medical follow-up study confirmed the clinical finding. The more rapid the involuntary movements, the better was the effect of subthalamotomy. Intention tremor was the most suitable symptom for stereotaxic treatment. Rigidospasticity was alleviated in the distal muscles only. Speech and gait were often improved. Good results were obtained in patients with normal intelligence, while feeble-minded patients did not benefit from the operation. Subthalamotomy was followed by transitory side-effects in 20%, of which increased involuntary movements on the ipsilateral side and mental restlessness were the most frequent. The operation had no mortality. Spinal longitudinal myelotomy effectively relieved the spasticity of the lower limbs in the three patients operated upon.


The treatment of total urinary incontinence.


Lymphographic visualization of lymphaticovenous communications and their significance in malignant hemolymphopathies.


History of the Medical Society of the District of Columbia. Chapter 3. Public Health.


Dr. Georg Neubauer.

Dr. Georg Neubauer.


A model for documentation of spina bifida.


An outline of recent work on the spinal cord of the cat.


Computer-aided diagnosis in pediatrics.

Computer-aided diagnosis in pediatrics.


Junctional rhythms.

Junctional rhythms.


The Wolff-Parkinson-White syndrome: evolving concepts of its pathogenesis.


The electrocardiogram in patients with pacemakers.


The electrocardiogram and the central nervous system.


John Swett's experience with physical exercise at the Rincon School: foundation for the first state physical education law in the U.S.


Maryland colonials at play: their sports and games.


Current bibliography on allergy and applied immunology.


Anorexia nervosa: a review.

Anorexia nervosa: a review.


The importance of chloroquine resistant malaria in Singapore in 1969.


The development of the heart-lung apparatus.


Haemostasis in the pig after transplantation of stored livers.


John H. Gibbon, Jr., M.D.

John H. Gibbon, Jr., M.D.


Spontaneous intracerebral haemorrhage. A review.


Mongoose rabies in Grenada.

Mongoose rabies in Grenada.


From antisepsis to asepsis. Louis Pasteur's publication on "The germ theory and its application to medicine and surgery".


Léon Bernard Foundation Award. Dr. Arcot Lakshmanaswami Mudaliar.


Shousha Foundation Award. Dr. Sabih Al-Wahbi.


Gaba derivative in spasticity. (Beta-(4-chlorophenyl)-gamma-aminobutyric acid, Ciba 34.647-Ba).


Headache and treatment with oral contraceptives.


Transabdominal anterior interbody fusion of spondylolisthesis L5-S1.


Clinical, biochemical, and experimental studies on lactation. V. Clinical effects of steroids on the initiation of lactation.


The literature of ethical problems in medicine. 2.


The spectrum of aspiration pneumonitis.

The spectrum of aspiration pneumonitis.


Ultrastructure of the tegmentum vasculosum and transitional zone.


Cortisol production in depressive illness. A clinical and biochemical clarification.


Probe: a computer instrument for field surveys of psychiatric disorder.


Differentiation of the "giving-up" affects--helplessness and hopelessness.


Pathologic quiz case.

Pathologic quiz case.


Gene expression in bacteria and some concerns about the misuse of science.


H. A. Krebs: a pathway in metabolism.

H. A. Krebs: a pathway in metabolism.


Immunization against infectious diseases.


The search for protective antigens.

The search for protective antigens.


Immunization against smallpox.

Immunization against smallpox.


Biological activity of bacteriophage ghosts and "take-over" of host functions by bacteriophage.


Measles vaccines.

Measles vaccines.


Rabies vaccines.

Rabies vaccines.


Rubella vaccines.

Rubella vaccines.


Diphtheria and tetanus toxoids.

Diphtheria and tetanus toxoids.


Pertussis, typhoid-paratyphoid and cholera vaccines.


Safety of vaccines.

Safety of vaccines.


Immunization against protozoal diseases.


Viable count of bacteria by agar-block-spread method.


The case of the two "Huartes": a historical confusion.


"Education for morality". Sex education--Victorian style.


Further observations on respiratory flow-volume loops: clinical evaluation of a new oral bronchodilator, CP-12,521-1.


History of Canadian surgery. Organization man.


The metabolic response to pentazocine as a supplement to balanced anaesthesia for major abdominal surgery.


Gastric mucin and mucinous secretions.

Gastric mucin and mucinous secretions.


Combined abdominoperineal excision for cancer of the rectum: primary suture of the perineal incision with continuous suction.


Basic principles of electrosurgery: a review.


Possible immunity in man to Trypanosoma Rhodesiense. Estimation of antibody titre by fluorescent antibody test.


Regina Coeli Mission Hospital Nyamaropa Tribal Trust Land.


Richard Lionel Spittel.

Richard Lionel Spittel.


The first Sir Marcus Fernando Memorial oration.


Professor O. E. R. Abhayaratne.

Professor O. E. R. Abhayaratne.


The management of acute respiratory failure.


Observation on the mechanism of renin release by hydralazine in hypertensive patients.


Acute arthritis of the sternoclavicular joint due to coliform bacillus.


Jacobson v. Massachusetts 25 S. Ct. 358 (1905): compulsory vaccination.


Platelet function: its clinical significance.


Kinetics of polymerization and phosphorolysis reactions of Escherichia coli polynucleotide phosphorylase. Evidence for multiple binding of polynucleotide in phosphorolysis.


Regulation of pyruvate dehydrogenase complex synthesis in Escherichia coli K 12. Identification of the inducing metabolite.


The murein-lipoprotein linkage in the cell wall of Escherichia coli.


Immunochemical studies on core lipopolysaccharides of Enterobacteriaceae of different genera.


Diaphragmatic paralysis following herpes zoster.


A bacteriological study in women with early post-partum intrauterine contraceptive device insertion.


Presidential acceptance address.

Presidential acceptance address.


Gastroenterology in India--a review (1945-1969).


Rheumatic and collagen diseases. Review of scientific papers published during a 25-year period, 1944-69.


Kidney. Review--Indian publications--25 years: 1945-1969.


Infectious diseases. A review of work done in India.


The amino acid sequence of beta galactosidase. I. Isolation and composition of tryptic peptides.


Simple method for skin grafting in mice.


Computer diagnosis.

Computer diagnosis.


On the use of radioisotopes in human experimentation.


Hemispheric cerebral dominance.

Hemispheric cerebral dominance.


Urinary ascites present at birth associated with lumbosacral myelomeningocele.


International abstracts of pediatric surgery.


Edward Joseph Donovan 1889-1970.

Edward Joseph Donovan 1889-1970.


Foreward and Publications by Balint Orban.


Histogenesis of repair following osseous surgery.


Free gingival grafts in humans.

Free gingival grafts in humans.


Iliac transplants in periodontal therapy.


The fluorescent antibody technique. Its application to the detection of blood group antigens in stains.


Epithelial and connective tissue changes in periodontitis.


The induction of new bone and cementum formation. 3. Utilizing bone and marrow allografts in dogs.


Studies on haptoglobin synthesis in reticuloendothelial tissues.


Early reconstruction of the elbow following severe trauma.


Primary closure of open fractures of the tibia and fibula by fibular fixation and relaxing incisions.


Collective review--systemic response to burn injury: a survey of some current concepts.


Popliteal artery exploration in aortographies with no lower limb vessels filling.


Effect of adrenaline on glucose oxidation by pancreatic beta-cells.


Dr. Shaw "revives" Captain Kennedy.

Dr. Shaw "revives" Captain Kennedy.


Festschrift in honor of EB Astwood, MD, CM, PhD.


Effect of aminoglutethimide and reserpine on the human pituitary-adrenal axis: remission of a case of Cushing's disease.


A comparison of response of serum growth hormone to glucagon and vasopressin.


An abortive attempt. How a meeting of medical men met in 1883 hoping to establish a medical association in Winnipeg.


Bibliography of E. B. Astwood.

Bibliography of E. B. Astwood.


Effect of exogenous glucagon on pituitary polypeptide hormone release.


Twenty-year experience with Malaria at U.S. Public Health Service Hospital.


Human breast carcinoma. 3. Cellular responses to autologous breast cancer: skin-window procedure.


Ball combined cystopexy.

Ball combined cystopexy.


The below-knee amputee with skin grafts.


Selection of lungs for transplantation. Development of methods of rapid production of specific immunologic tolerance is suggested.


Experimental and clinical lung transplantation. Further trials in human transplantation are justified, but many problems remain unsolved.


Some notes on a centenary.

Some notes on a centenary.


Value of antibiotics, corticosteroids, and peritoneal lavage in the treatment of experimental peritonitis.


Approach to fluid dynamic problems in reconstructive vascular surgery.


Drug-resistant tuberculosis: a review of the world situation.


Clinical applications of ethambutol.

Clinical applications of ethambutol.


Ethambutol: a review with special reference to ocular toxicity.


The use of ethambutol in clinical practice.


"Atypical" mycobacteria: a review.

"Atypical" mycobacteria: a review.


The anatomy of the tarsi of Schistocerca gregaria Forskål.


Two useful variations of the Golgi silver chromate method.


Renal biopsy in children's Schoenlein-Henoch syndrome.


The effect of glucose on insulin biosynthesis by isolated islets of Langerhans of the rat.


Enthalpy-entropy compensation phenomena in water solutions of proteins and small molecules: a ubiquitous property of water.


Mycobactins: iron-chelating growth factors from mycobacteria.


Bacterial growth and the cell envelope.

Bacterial growth and the cell envelope.


David A. Karnofsky memorial lecture. Thoughts on chemical therapy.


The Medical Council of Rhodesia--its history.


Lymphoma of the testis.

Lymphoma of the testis.


Current linkage map of Salmonella typhimurium.


The chemistry of thiadiazole and thiadiazine S-oxides.


Antibacterial action of copper in respiratory therapy apparatus.


Current linkage map of Escherichia coli.


The origins of behavior modification with exceptional children.


Alcoholism. Resources for helping the problem drinker.


Incidence and nature of urinary tract infections among asymptomatic primary school children.


M. S. Haro appointed ADA Bureau Director.


Giovanni Cambosu.

Giovanni Cambosu.


Laminar air purge of microorganisms in dental aerosols.


Roster. Louisiana State Medical Society.


The Louisiana State Medical Society. Of service since 1878.


Jerome Cochran lecture. Development of arterial grafts and arterial surgery in Alabama.


Menetrier's disease. Report of a case with review of the literature.


History of the Medical Society of the District of Columbia.


R. Austin Freeman and Dr. Thorndyke. Forensic detection.


Abraham Jacobi, M.D. 1830-1919, founder of specialty of pediatrics and teacher, writer, lecturer, and humanitarian.


Correlation of somatic, biochemical, perceptual, and Rorschach data on 40 healthy male subjects.


Determination and transdetermination in cells of the fruitfly.


Techniques and apparatus for the study of visual perception.


Infections produced by S. panama.

Infections produced by S. panama.


Freud as philosopher.

Freud as philosopher.


Thyroid as an adjuvant to amphetamine therapy of obesity. A controlled double-blind study.


American Association of Anatomists. List of members.


The organ of Langerhans in new perspective.


Folate deficiency in premature infants.

Folate deficiency in premature infants.


Ventilator therapy in the newborn. Some mechanical considerations.


Medium chain triglycerides in paediatric practice.


Electron microscopic investigations on the Langerhans islets of the golden hamster.


Hyperparathyroidism. Some early patients.


Chondroitin therapy.

Chondroitin therapy.


Alternate-day vs shorter-interval steroid administration.


Frequency of arthralgia in women receiving one of three rubella vaccines.


Experience counting in sequential computer diagnosis.


Taxonorics. I. Formulation of criteria.

Taxonorics. I. Formulation of criteria.


Colin Campbell Anderson.

Colin Campbell Anderson.


The archives of the college. The radium certificates in the Sear collection.


Röntgen oration, 1969: the new photographers.


Long time results of respirator therapy.


Mechanical complications of artificial ventilation in the newborn.


Results of artificial ventilation in the newborn.


Artificial ventilation in hyaline membrane disease. Analysis of a series (130 cases).


Positive-pressure respirators, and description of the French machine RPR.


Symposium on artificial ventilation. Summary of conference proceedings.


Methods of artificial ventilation in the newborn (IPPV).


Circulatory studies in clinical hyaline membrane disease.


Trial of Chymoral in professional footballers.


A biopsy study of the innervation of forearm skin grafted to the finger tip.


Acid maltase deficiency in adults: studies in four cases of a syndrome which may mimic muscular dystrophy or other myopathies.


Determination of fetal, placental and neonatal blood volumes in the sheep.


Pressure-dependent heterogeneity of renal cortical blood flow in dogs.


A cooperative kidney typing and exchange program.


The incubation of unfixed tissues for electron microscopic histochemistry.


Determining Feulgen-DNA of individual chromosomes by fluorescence cytophotometry with incident light.


Our new president.

Our new president.


Preleukemia: A model for investigation into the etiology and treatment of leukemia.


The state: an ecological phenomenon.

The state: an ecological phenomenon.


Prevention of rheumatif fever and rheumatic heart disease.


Blood letting. Barber-surgeons' shaving and bleeding bowls.


Sir William Jenner (1815-1898).

Sir William Jenner (1815-1898).


Some logistics of tissue typing for cadaver kidney transplantation in humans.


Lasers in medicine and biology.

Lasers in medicine and biology.


Obituary. Hugh Stewart Douglas.

Obituary. Hugh Stewart Douglas.


Franz Kral.

Franz Kral.


James Dewar Hunter.

James Dewar Hunter.


Thomas Fletcher Telford.

Thomas Fletcher Telford.


The role of prostaglandins in reproductive physiology. Research on the physiopathologic and biochemical nature of prostaglandins (PGs) suggest that PGs play a role in reproductive physiology.  In vitro studies show that the PGE series decrease the motility of the human uterus, fallopian tubes, and ureter, and produce vasodilatation.  PGFs cause vasoconstriction and increased motility of the uterus, fallopian tubes, ureter, and gastrointestinal muscle.  PGs are also known to inhibit lipolysis, platelet aggregation, and gastric secretion.  The exact mechanism of PGs are not fully understood, but evidence suggests that many responses can be attributed to interference with the enzyme adenyl cyclase, which catalyzes the formation of adenosine 3',5'-monophosphate (cyclic AMP) from adenosine triphosphate.  The adenyl cyclase-cyclic AMP system mediates lipolysis, steroidogenesis, gastric secretion, certain smooth muscle motility responses, and increase in permeability due to vasopressin.  Early studies of the myometrial effects of PGs showed that the PGE series inhibited the motility of the human myometrium in vitro while the PGF series produced mixed responses.  The role of PGF2alpha in parturition has not been established but evidence suggests that it has a potential role as an oxytocic in cases of therapeutic abortion.  In the area of human fertility, the physiologic role of PGs in seminal fluid is hypothesized to facilitate the migration of spermatozoa from the vagina into the uterine cavity.  Karolinska Institute researchers have found that some infertile males have low PG levels in their ejaculates and are now working with methods of improving the PG levels to improve their fertility.  Pickles et al. proposed a potential role for PGs in the etiology of dysmenorrhea, having found a significantly higher ratio of PGF to PGE in a series of patients with severe dysmenorrhea than in a comparable series of normal patients.  The luteolytic and antinidatory effects of PGF2alpha are being investigated and studies appear encouraging.  PGs have therapeutic potentials in induction of labor, treatment of infertility, morning-after conception, treatment of dysmenorrhea, and contraception by alteration of fallopian tube motility.


Circulatory effects of estrogen.

Circulatory effects of estrogen.


General practitioner of the year. Thomas E. Rardin, M.D. receives first award. Future awards will be presented in his honor.


Highlights in Ohio welfare. Functions and structure of the division of medical assistance.


Demulen: hastily approved drug.

Demulen: hastily approved drug.


Nobel Peace Prize: developer of high-yield wheat receives award (Norman Ernest Borlaug).


The cultural basis for our environmental crisis. The forces of democracy, technology, urbanization, increasing individual wealth, and an aggressive attitude toward nature seem to be directly related to the environmental crisis now being confronted in the Western world. The Judeo-Christian tradition has probably influenced the character of each of these forces. However, to isolate religious tradition as a cultural component and to contend that it is the "historical root of our ecological crisis" is a bold affirmation for which there is little historical or scientific support.


Rhizosphere microflora of leguminous plants.


Studies on composting Tripoli town refuse with special reference to the Erap-Thomas rapid method.


Laboratory suggestion: apparatus for incubating and washing slides in immunofluorescent preparations.


Donald Hunter Kaump, M.D., 1907-1970.

Donald Hunter Kaump, M.D., 1907-1970.


In memoriam. Ernest Witebsky, M.D., 1901-1969.


What is the history of psychiatry?

What is the history of psychiatry?


The medicine machine versus today's superspecialist--a study in anachronization.


Methylphenidate and thioridazine: learning, reaction time, activity, and classroom behavior in disturbed children.


The mutual storytelling technique. Use in the treatment of a child with post-traumatic neurosis.


Alfred Adler in historical perspective.

Alfred Adler in historical perspective.


Thyroglossal tract carcinoma. A review of the literature and addition of eighteen cases.


Mendelson's syndrome. Diagnosis, therapy, and prevention.


A needle designed for suture ligation of a bleeding duodenal ulcer.


Total exchangeable sodium in chronic nephropathy with and without hypertension.


Carbohydrates of the glycopeptides released by the action of rennin on whole milk.


Durkheim and contemporary social pathology.


[Centenial of the birth of Professor Jules Gonin].


A contour study of the carotid pulse in normal subjects, aortic valvular disease, and hypertrophic subaortic stenosis.


Reduction in the activity of the pancreatic islets induced in normal rodents by prolonged treatment with derivatives of sulfonylurea.


Loss of T antigen by cells derived from a hamster tumour induced by adenovirus type 12.


Effect of streptozotocin-induced diabetes on pancreatic insulin content of the fetus.


Histopathology of skin cancer following cryosurgery. Preliminary report.


Enzymatic synthesis of deoxyribonucleic acid. XXXV. A 3'-hydroxylribonucleotide binding site of Escherichia coli deoxyribonucleic acid polymerase.


Properties of thymidylate synthetase from dichloromethotrexate-resistant Lactobacillus casei.


Identification of flavin adenine dinucleotide and heme in a homogeneous spermidine dehydrogenase from Serratia marcescens.


Isolation and characterization of arginine-inducible acetylornithine delta-transaminase from Escherichia coli.


Formation of randomly paired disulfide bonds in des-(121-124)-ribonuclease after reduction and reoxidation.


Acyl carrier protein. XIV. Further studies on beta-ketoacyl acyl carrier protein synthetase from Escherichia coli.


Spondylolisthesis and tight hamstrings.

Spondylolisthesis and tight hamstrings.


Paralysis of hip abductor muscles in spina bifida. Results of treatment by the Mustard procedure.


James Spencer Speed, M.D. 1890-1970.

James Spencer Speed, M.D. 1890-1970.


Blastomycosis of the knee joint. Case report.


John William Hillman, M.D. 1921-1970.

John William Hillman, M.D. 1921-1970.


The treatment of vaginitis with quinettes--a clinical study.


Effect of Friend leukemogenic virus on antibody-forming cells to a bacterial somatic antigen.


Patton--and the slap heard 'round the world.


Woodrow Wilson: the ordeal of his second term.


What about renal transplantation?

What about renal transplantation?


Hahnemann interprets EKG in one minute with computer.


Treatment with intranasal dexamethasone sodium phosphate.


Study of the antimicrobial action of pectin. II. Factors affecting the bactericidal activity of pectin.


Automated multitest facilities.

Automated multitest facilities.


Officers (1970-71) specialty societies affiliated with the institute of medicine of Chicago.


The Institute of Medicine of Chicago.

The Institute of Medicine of Chicago.


The management of hand injuries.

The management of hand injuries.


Distribution of pulmonary blood flow before and after autotransplantation measured with xenon-133.


Lung transplantation and pulmonary arteriolar circulation.


Treatment of a case of combined Mallory-Weiss and Boerhaave's syndrome.


Choice of operation for the toxic megacolon phase of nonspecific ulcerative colitis.


Successful management of an injury to the suprarenal inferior vena cava.


Emergency reconstruction of the superior vena cava.


Growth factor requirement of Shigella flexneri and Shigella sonnei.


Blood chemical and pancreatic histologic alterations in alloxan-diabetic ewes and their fetuses.


Pathologic features of picornavirus infections in cats.


Mastitis in guinea pigs.

Mastitis in guinea pigs.


Salmonella dublin as a cause of diarrhea and abortion in ewes.


Characterization of five clostridial pathogens by gas-liquid chromatography.


The effect of mechanical ventilation after open-heart surgery.


The A. Charles King collection of early anaesthetic apparatus.


Danger lists for the anaesthetist. A revised version.


The microcirculation of the bone marrow.


C cell follicles in the dog thyroid: demonstration by in vivo perfusion.


Fine structure of the epithelial dental organ in the frog during early odontogenesis.


Clostridium formicoaceticum nov. spec. isolation, description and distinction from C. aceticum and C. thermoaceticum.


The aerobic decomposition of choline by microorganisms. I. The ability of aerobic organisms, particularly coryneform bacteria, to utilize choline as the sole carbon and nitrogen source.


Medical department of the United Verde Copper Company.


Clinical and quantitative electroencephalographical effects of MK 940.


Skull reconstruction with bone dust.

Skull reconstruction with bone dust.


A historical review of the use of local physical signs in burns.


Problem of sensory loss in finger-tip injuries in the blind.


The use of the intact side of the face for reconstruction of the injured opposite side.


The effect of 5-fluorouracil on direct and developed spleen hemolysin plaque-forming cells of mice.


The value of human abortuses in the surveillance of developmental anomalies. II. Reduction deformities of the limbs.


On the possibilities of induction of the pituitary prolactin secretion by means of exogenous prolactin.


Immunofluorescent study of the blood-group antigens A and B in the buccal cells of human secretors and non-secretors.


Directory.

Directory.


The method for estimation of opsonic activity in sera of infants.


Simplified technique for collection of preputial samples from bulls for isolation of Vibrio fetus.


Growth hormone influences on palmitate entry rate in fed and fasted sheep.


Effects of supplemental potassium bicarbonate upon 24Na and 42K metabolism in young nonlactating cows.


Recent developments in cobalt and copper in ruminant nutrition: a review.


Metabolism of iron and manganese.

Metabolism of iron and manganese.


Zinc nutrition of cattle: a review.

Zinc nutrition of cattle: a review.


Selenium.

Selenium.


Iodine.

Iodine.


A five year survey in Cork of spina bifida and hydrocephaly.


Spina bifida in Ireland.

Spina bifida in Ireland.


Carter Godwin Woodson, Ph.D., LL.D., 1875-1950. The father of negro history.


The macrophage in cellular and humoral immunity.


Giessen University: historical and current aspects.


The molecular parameters of olfactory response.


Determinants of the response to oral anticoagulant drugs in man.


Experimental drowning: coronary haemodynamics and myocardial metabolism in the dog.


Perfusion of the isolated pig liver. I. Mechanical characteristics of the circuit.


Mutagen specificity.

Mutagen specificity.


Beginnings of greatness in Swedish Chemistry. II. Axel Fredrick Cronstedt (1722-1765).


The anatomical area of involvement in streptococcal infections and the carrier state.


Retrospect and tribute: remarks in acceptance of the C. Anderson Aldrich Award.


The pyrogenic effect of scarlet fever toxin. IV. Pyrogenicity of strain C 203 U filtrate: comparison with some basic characteristics of the known types of scarlet fever toxin.


Acino-insular cells in normal rat pancreas.


External diseases of the eye (excluding the cornea)--an annual review of the ophthalmological literature.


Antimicrobial activity of 3-substituted 5-amino-1,2,4-oxadiazoles.


Phosphonomycin. 3. Evaluation in vitro.

Phosphonomycin. 3. Evaluation in vitro.


Efficacy of phosphonomycin in treatment of urinary-tract infections.


Clinical studies with the Cendehill strain of attenuated rubella vaccine.


Clinical trials with Cendehill strain attenuated rubella virus vaccine.


Antibody response and reactions to Cendehill rubella vaccine in adult women.


Amantadine therapy of epidemic influenza A2-Hong Kong.


Clinical and laboratory evaluation of cephalexin in urinary-tract infections.


Controlled prospective double-blind evaluation of a "prophylactic" antibiotic (cephaloridine) in surgery.


Pharmacokinetic interpretation of penicillin levels in serum and urine after intravenous administration.


In vitro susceptibility patterns of beta-hemolytic streptococci.


Effect of parenteral iron preparations on renal infection due to Escherichia coli in rats and mice.


Mode of action of azotomycin.

Mode of action of azotomycin.


Pharmacokinetic interpretation of dicloxacillin levels in serum after extravascular administration.


Evaluation of an automated fluorescent treponemal antibody test for syphilis.


Fluorescent treponemal antibody absorption (FTA-ABS) test in yaws.


Evaluation of a modified FTA-ABS test multispot FTA-ABS.


Comparison between the results of the Brewer rapid plasma reagin card test and other tests for syphilis.


Fever after inoculation of rabbits with Treponema pallidum.


Reactivity in the FTA-ABS test of rabbits hyperimmunized with nonpathogenic treponemes.


Double-blind or not?

Double-blind or not?


Lung transplantation reviewed.

Lung transplantation reviewed.


Reduction of reaction due to iron dextran infusion using chloroquine. The use of promethazine and chloroquine sulphate to reduce reaction to total dose infusion of iron dextran (Imferon) is described. The patients chosen for the investigation were anaemic pregnant African women living in the coastal region of East Africa where malaria due to Plasmodium falciparum is holoendemic. The results show that promethazine has little value in the reduction of reactions whereas chloroquine appears to be effective.


Assessing reports of therapeutic trials. A check list is described which helps the systematic assessment of reports of therapeutic trials, particularly the aspects that need to be considered in assessing their validity. The check list was used to examine 141 reports of therapeutic trials published in four British non-specialist journals in the first six months of 1966 and of 1969. Of these reports 51% were found to be acceptable and a further 16% probably acceptable; 33% were considered unacceptable because they lacked one or more of the features required in a valid report. The check list has been found useful in assessing claims made for drugs and other therapeutic measures on the basis of published reports.


How old is leprosy?

How old is leprosy?


Colombo Medical School Centenary.

Colombo Medical School Centenary.


Tissue typing, antilymphocyte globulin, and prophylactic graft irradiation in cadaver kidney transplantation. In a series of 27 recipients of cadaver kidney grafts, 26 were at the time of writing alive, 3 to 25 months after transplantation, and 25 patients were alive with functioning first grafts. The one-year patient survival in 18 patients was 94% and the one-year graft survival was 89%. There was no beneficial correlation between tissue matching and the frequency of major early rejection episodes or graft function 12 or more months after transplantation. Antilymphocyte globulin administration was associated with a lower incidence of early rejection episodes, but this was not statistically significant. A combination of prophylactic graft irradiation and antilymphocyte globulin administration for at least the first two weeks was associated with a significantly reduced frequency of major early rejection episodes and appreciably better graft function at 12 months. This effect could not be ascribed to better tissue matching.


Protection of man from natural infection with influenza A2 Hong Kong virus by amantadine: a controlled field trial. Prophylactic administration of amantadine in doses of 100 mg. twice a day offered statistically significant protection against influenza A2 infection in a double-blind field trial involving 391 medical student volunteers during the influenza A2 Hong Kong epidemic in Helsinki in the winter of 1969. Serologically verified influenza, as measured by complement fixation and/or haemagglutination inhibition, occurred in 27 out of 192 students in the amantadine group against 57 out of 199 in the placebo group, giving a protection rate of 52%.


A controlled study of L-DOPA in Parkinson's disease. A small group of patients with Parkinson's disease were treated with oral l-dopa. In ten cases the drug was given according to a randomized, double-blind-crossover protocol, and in 13 cases both the physician and the patient knew what treatment was being given. With subjective bias at a minimum the salutary effects of the amino acid were confirmed in 90 percent of the patients. The general level of improvement with l-dopa was better than with standard agents, and a combination of drugs was sometimes very helpful. Toxic psychosis was a problem in three cases, but other adverse reactions were minimal. Further extensive trial and study is indicated.


Prevalence of types of Streptococcus pyogenes and their susceptibility to 30 antibiotics.


Incidence of resistant pulmonary tuberculosis in relation to initial bacterial load, Rifampicin and INH in experimental tuberculosis.


Progress in birth defects research.

Progress in birth defects research.


Ontogeny of the immune response in animals having less than one million lymphocytes: the larvae of the toad Alytes obstetricans.


Effect of mouse submandibular gland extracts on survival of H-2 incompatible skin allografts.


Glucose-induced resistance to gamma-rays in Escherichia coli.


Response of rat blood, spleen, and lymph node leucocytes to soluble and insoluble antigen.


The effects of steroid hormones on macrophage activity.


Strandedness of chromosomes.

Strandedness of chromosomes.


The fine structure of malaria parasites.


Isozymes: classification, frequency, and significance.


Protein metabolism in nerve cells.

Protein metabolism in nerve cells.


The significance of the structural and functional similarities of bacteria and mitochondria.


Mechanism of chlorpropamide antidiuresis in diabetes insipidus.


Effect of dexamethasone on plasma growth hormone levels in acromegaly.


Comments on a proposed new way of measuring thyrotropin (TSH) reserve.


Total urinary hydroxyproline excretion after administration of Vitamin D to patients with hypoparathyroidism.


Demonstration of homocytotropic reaginlike antibodies in mice and rabbits infected with Trichinella spiralis.


Further studies on the Plasmodium berghei-Anopheles stephensi--rodent system of mammalian malaria.


The development of Plasmodium gallinaceum Brumpt in the hemocoels of refractory Culex pipiens pipiens Linn. and susceptible Aedes aegypti (Linn.).


Fate of some culture flagellates in the hemocoel of Rhodnius prolixus.


Gametogenesis and chromosomal complement in Strongyloides ratti (Nematoda: Rhabdiasoidea).


Surgery of the cervical carotid artery. Neurological sequelae following unilateral occlusion of the carotid artery.


Septal dermoplasty--its several uses.

Septal dermoplasty--its several uses.


Acute injuries of the larynx and trachea in 49 patients. (Observations over a 15-year period).


Directory of Otolaryngologic societies. (Secretaries of the various societies are requested to keep this information up to date).


Historic hospitals. Bartholomew's Hospital of London.


Thrombus formation on polymeric materials. II. Blood compatible surfaces.


Historical hospitals. First hospital in the New World. Hospital of San Nicolas de Bari, Santo Domingo.


The evolution of pathology.

The evolution of pathology.


Volume of ascitic fluid.

Volume of ascitic fluid.


Thrombus formation of polymeric materials. 3. Mechanism of blood coagulation.


Cellular deformability during maturation of the myeloblast. Possible role in marrow egress.


Bones, stones and hormones: the contributions of Fuller Albright.


Artificial cardiac pacemakers. II.

Artificial cardiac pacemakers. II.


Alice Hamilton--1869-1970.

Alice Hamilton--1869-1970.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 45-1970.


Artificial cardiac pacemakers. 3.

Artificial cardiac pacemakers. 3.


Studies on the surfaces of cercariae and schistosomula of Schistosoma mansoni.


Propagation of Plasmodium berghei and P. vinckei in mouse erythrocytes injected into chick embryos.


Thyroid peroxidase and thyroxine biosynthesis.


Pituitary and gonadal hormones in women during spontaneous and induced ovulatory cycles.


Multiple hormone interactions in the development of mammary gland in vitro.


Aspects of androgen-dependent events as studied by antiandrogens.


Study of spermatogenesis and steroid metabolism in cultures of mammalian testes.


Joseph Coats (1846-99) of Glasgow and the theory of cancer metastasis.


Postgraduate medical degrees of the University of Aberdeen, 1931-69.


The surgery of mitral incompetence.

The surgery of mitral incompetence.


Mechanisms regulating the menstrual cycle in women.


Fertility after renal transplantation.

Fertility after renal transplantation.


Late pulmonary function after reimplantation and allotransplantation of the lung in dogs.


Studies of the macrophage-inhibition test. 3. Use of the macrophage migration-inhibition technique in detection of active graft immunity.


A macro-autoradiographic method for the demonstration of iron-concentrating spleen colonies.


Prolongation of skin homograft survival by L-asparaginase.


Scanning and transmission electron microscopy of human ejaculate spermatozoa with special reference to their abnormal forms.


Vomeronasal receptors in turtles.

Vomeronasal receptors in turtles.


Further observations on dividing and non-dividing cnidoblasts in the regenerating isolated gastrodermis of Hydra.


The ultrastructure of human fetal odontoblasts.


The fine structure of pinacocytes in the marine sponge Microciona prolifera (Ellis and Solander).


Mechanism of conjugation and recombination in bacteria. XI. The relationship between entering of donor genophore and the initiation of female cell genophore replication.


Mechanism of conjugation and recombination in bacteria. XII. Susceptibility of donor DNA to 32P-decay during conjugation. The new model of recombination involving single-strandedness of donor DNA and triplex formation.


Acetylornithine deacetylase synthesis in Escherichia coli K12 and W in the presence of different concentrations of corepressor.


Use of amantadine in Parkinson's disease. Results of a double-blind trial. In a four-week double-blind trial of 62 patients with Parkinsonism 29 were treated withamantadine and 33 with a placebo. Modest but statistically significant improvement was observed in the first group, optimum benefit occurring after the first two weeks. Patients' reactions to the drug were favourable in 79%, and side effects were insignificant. Though amantadine appears to be a useful additive drug in the treatment of Parkinsonism, its value as a single treatment is as yet undetermined.


Infections of the urinary tract. II. Management.


Comparative trial of Nutrizym in chronic pancreatic insufficiency. A cross-over trial of pancreatic replacement therapy was carried out in 12 adults with chronic pancreatic insufficiency. The standard enteric-coated preparation, Pancrex V forte, was compared with Nutrizym, which has an enteric-coated core of pancreatic extract and a shell of bromelains-a mixture of proteolytic enzymes derived from the stem of the pineapple.Nutrizym was significantly more effective than Pancrex V forte in improving fat absorption, and reduced faecal weight. Protein digestion was assessed by measuring the urinary excretion of hydroxyproline after a gelatin meal. Nutrizym produced an earlier and significantly higher peak in hydroxyproline excretion than Pancrex V forte, but the cumulative effect was similar. The value of bromelains was investigated by including a period on the Nutrizym core alone. This was similar to Pancrex V forte in improving fat absorption but had less effect on protein digestion, suggesting that the beneficial effect of Nutrizym compared with Pancrex V forte was due to the added bromelains, and not to differences in enzyme content or enteric coating.


The enterocyte in coeliac disease.

The enterocyte in coeliac disease.


Cancer death rates by site and sex for religious and socioeconomic groups in New York City.


Stigma and the leprosy phenomenon: the social history of a disease in the nineteenth and twentieth centuries.


Influence of fasting on adrenocortical and pancreatic islet response to glucose loads in the obese.


Co-transcribed cistrons in bacteriophage T4.


On the effect of finite input on the (TRANS-CIS) yield ratio in bacteriophage complementation tests.


Transfer-defective mutants of sex factors in Escherichia coli. I. Defective mutants and complementation analysis.


Medicine in the canadian campaign of the revolutionary war: the journal of Doctor Samuel Fisk Merrick.


Baron Munchausen as an expert witness.

Baron Munchausen as an expert witness.


Transfer-defective mutants of sex factors in Escherichia coli. II. Deletion mutants of an F-prime and deletion mapping of cistrons involved in genetic transfer.


Histological methods of diagnosis of carp dropsy.


History of the exchange transfusion; its use in treatment of erythroblastosis fetalis.


A multisite-mutation map of the leucine operon of Salmonella typhimurium.


Allelism tests among various homothallism-controlling genes and gene systems in Saccharomyces.


F-prime factors of Salmonella typhimurium and an inversion between S. typhimurium and Escherichia coli.


Correspondence and reports. C.D. O'Malley. 1907-1970.


An interview with Dr. Karl Jaspers on wisdom and life.


Group work with the aged: selected literature.


Immunoglobulin spots on the surface of rabbit lymphocytes. Small and medium lymphocytes from the peripheral blood and lymphoid tissues of the rabbit react in suspension with antibodies directed against different immunoglobulin determinants. Through immunofluorescence, it was possible to show that numerous discrete spots on the surface of the positive lymphocytes carry immunoglobulin molecules. The positive lymphocytes are about one-half of all lymphocytes in the different preparations; thymus lymphocytes are all negative. With antisera specific for rabbit IgM as well as with antisera directed against allotypic determinants specific for IgM or IgG, it was possible to show that about nine-tenths of the immunoglobulin-positive lymphocytes carry IgM molecules on their surface. With antisera directed against a- and b-locus determinants, it was also possible to demonstrate that both heavy and light chains were present in the surface immunoglobulins. Furthermore, in animals which were heterozygous at the a or the b locus, it was found that each lymphocyte had immunoglobulins synthesized under the influence of only one of two alleles. A very small proportion of lymphocytes could be shown to have a specific surface reaction with one antigen (horse ferritin); the proportion of these cells increased very much after immunization.


Cellular and vascular components of the allograft reaction. Evidence from returned skin allografts. In order to gain added insight into the mechanisms of allograft destruction, skin grafts were returned to their original donors after remaining as allografts long enough to induce immunity in the intermediate host but not long enough to cause destruction of the graft. Upon their return to unmodified donors, such grafts became revascularized and remained viable. An intense cellular infiltration was incited within the graft and its draining lymph node by the interaction between immunologically competent cells, some antigenically activated, that were transferred from the intermediate host with the graft, and those of the final host, the original donor. This immune interaction excited a nonspecific granulocytic and histiocytic response, which led to the destruction of the adjacent epithelium already re-accepted within its native habitat. This mechanism of epithelial destruction required vascular connection to permit the cellular infiltration, and was unlikely to have primarily involved circulating antibody. When similar grafts were returned to donors that had been sensitized to the intermediate host, vascularization and reacceptance of the graft did not occur. No cellular infiltration took place in the graft and no lymph node response was evoked. The returned grafts were cast off as full-thickness sloughs. Here the mechanism of graft rejection was apparently an interaction between the preformed antibody of the specifically sensitized host and the allogeneic cells transferred from the intermediate host; this interaction prevented the vascularization of the graft, even though the endothelia involved were autologous. In unmodified allografts, both the character and the variability of the histologic patterns can be accounted for by the superimposition, in differing rates and degrees, of humoral vascular effects upon cellular events already in progress.


The abrogation of sheep erythrocyte tolerance in rats by means of the transfer of allogeneic lymphocytes. Whereas the transfer of lymphocytes from normal syngeneic donors fails to abrogate tolerance of sheep erythrocytes in rats, lymphocytes from allogeneic donors are effective. When tolerance is abrogated in this situation, the hemolysin-forming cells are predominantly of host origin. Immunological interaction between transfused lymphocytes and host cells is a prerequisite for the abrogation of tolerance. From the time required for abrogation to occur after transfer of the allogeneic cells, it is suggested that tolerance of sheep erythrocytes in rats represents the repression of a specific reactivity in cells rather than the elimination or irreversible inactivation of reactive cells. This explanation implies the existence of specifically tolerant cells.


Predominant contribution of IgA antibody-forming cells to an immune response detected in extraintestinal lymphoid tissues of germ-free mice exposed to antigen by the oral route.


Cell transfer studies on the persistence of homograft sensitivity in the mouse.


Effect of Tween 80 on strand breaks of Escherichia coli DNA irradiated in situ.


Antibody production by host cells in x-irradiated recipients of allogeneic spleen cells.


The isoxazolyl penicillins: oxacillin, cloxacillin, and dicloxacillin.


The clinical use of chloramphenicol.

The clinical use of chloramphenicol.


Kanamycin.

Kanamycin.


Bacitracin and tyrothricin.

Bacitracin and tyrothricin.


Lincomycin: fact, fancy, and future.

Lincomycin: fact, fancy, and future.


Erythromycin.

Erythromycin.


Gentamicin.

Gentamicin.


Nystatin.

Nystatin.


Histoplasmosis in India. A critical review.


Basic studies of the skin transplant for tympanoplasty.


Portrait of a student nurse.

Portrait of a student nurse.


Molecular biology: origin of the term.

Molecular biology: origin of the term.


Research on sugar nucleotides brings honor to Argentinian biochemist (Luis Leloir).


Mechanism for the water-to-air transfer and concentration of bacteria. Air bubbles breaking at the air-water interface can remove bacteria that concentrate in the surface microlayer and eject the bacteria into the atmosphere. The bacterial concentrations (numbers per milliliter) in the drops ejected from the bubbles may, depending on drop size, be from 10 to 1000 times that of the water in which the bubbles burst.


Tuberculin-active carbohydrate that induces inhibition of macrophage migration but not lymphocyte transformation. A tuberculin carbohydrate fraction, GAE, in sensitized animals induced a delayed type of skin reactivity and inhibited the migration of macrophages but failed to stimulate lymphocyte transformation in vitro. Tuberculin protein-containing fractions were active in each test. These results show that in vitro lymphocyte transformation is not necessarily a corollary of delayed type hypersensitivity.


Technique and results of human heart transplantation.


Liver transplantation.

Liver transplantation.


Immunosuppression and neoplasia.

Immunosuppression and neoplasia.


Treatment of resistant schizophrenics with extreme high dosage fluphenazine hydrochloride.


Imipramine pamoate in hospitalized depressives: a double-blind comparison with placebo.


Pancreatico-duodenal and renal allotransplantation in juvenile onset, insulin dependent, diabetes mellitus with terminal nephropathy.


Current status of development of artificial organs.


The metabolism of glucose, acetate, palmitate, stearate and oleate in pigs.


Cyclotrons in nuclear medicine.

Cyclotrons in nuclear medicine.


A comparative statistical analysis of brain scans using a digital computer.


Mikolaj Rejchman, M.D.

Mikolaj Rejchman, M.D.


Lung changes associated with organ transplantation.


Recent advances in molecular pathology:  a review. Cholesterol flux and metabolism in arterial tissue and in atheromata.


The amino acid sequence of T4 phage lysozyme. 3. Peptic digestion.


A study of the sulfhydryl groups of the catalytic subunit of Escherichia coli aspartate transcarbamylase. The use of enzyme--5-thio-2-nitrobenzoate mixed disulfides as intermediates in modifying enzyme sulfhydryl groups.


Enzymatic joining of polynucleotides. IX. A simple and rapid assay of polynucleotide joining (ligase) activity by measurement of circle formation from linear deoxyadenylate-deoxythymidylate copolymer.


Crystalline L-asparaginase from Escherichia coli B. I. Purification and chemical characterization.


Crystalline L-asparaginase from Escherichia coli B. II. Physical properties, subunits, and reconstitution behavior.


Edward Nettleship (1845-1913) veterinarian-dermatologist-ophthalmologist-geneticist.


Incidence of posttransfusion hepatitis in previously frozen blood.


Current concepts. The kidney in pregnancy.


Rheumatic fever and throat culture.

Rheumatic fever and throat culture.


Erythropoietin alterations in dogs with twenty-four-hour preserved kidneys.


Recovery from hepatic coma and respiratory arrest with help of positive-pressure ventilation and intermittent plasma exchange.


Parkinson's disease. I.

Parkinson's disease. I.


Tolerance to allogeneous grafts induced by viral antigens.


Historical and oecological observations on plague in the United States.


Treatment of long-term tinea pedis with miconazole. Double-blind clinical evaluation.


Bullous pemphigoid following the topical use of fluorouracil.


Urinary excretion and plasma levels of sulphonamides in patients with renal impairment.


Aspirin, ethanol and the stomach.

Aspirin, ethanol and the stomach.


Functional anatomy of the dentate nucleus in the light of stereotaxic operations.


Immunofluorescence studies on the distribution of gastrin cells in different clinical states. Examination of human gastric biopsies by an indirect immunofluorescence technique using antihuman gastrin confirms the variability in distribution and number of gastrin-secreting (G) cells (Solcia, Vassallo, and Capella, 1969) observed by morphological, cytochemical, and electron microscopical studies. In cases with presumed secondary hypersecretion a profound degree of G-cell hyperplasia is observed. Immunofluorescence with. antigastrin can provide valuable information on the state of the G cells in different physiological states, particularly if used in conjunction with other cytochemical techniques.


The endocrine polypeptide cells of the human stomach, duodenum, and jejunum. Thirty specimens of stomach, duodenum, and jejunum, removed at operation, were examined by optical microscopical, cytochemical, and electron microscopical techniques. The overall distribution of four types of endocrine polypeptide cell in the stomach, and three in the intestine, was determined. The seven cell types are described by names and letters belonging to a scheme for nomenclature agreed upon at the 1969 Wiesbaden conference on gastrointestinal hormones. The gastrin-secreting G cell was the only cell for which firm identification with a known hormone was possible. Although there was wide variation in the distribution of the various cells, from one case to another, striking differences were nevertheless observable, with respect to the G cell, between antra from carcinoma and from ulcer cases.


Primary lymphoma of the upper small intestine. Seven patients with primary lymphoma involving the upper small intestine and presenting with diarrhoea, non-specific abdominal pain, and clubbing are reported. The disease appears to be more prevalent in young women, and clinical and radiological findings can provide an excellent preliminary diagnosis which is usually confirmed by peroral biopsy of the small intestine. This type of lymphoma is found to be clinically distinguishable both from the primary intestinal lymphomas reported from western countries and also from gastrointestinal involvement as part of a more systemic disease. It appears to be prevalent in the Middle East, and because of clear clinical, radiological, and histological features, it can be singled out from other primary intestinal lymphomas and considered as a distinct clinical entity.


Gastric ulcer and regurgitation gastritis.


An analysis of the recovery of tetrahymena from effects of cycloheximide.


Intestinal peptidases.

Intestinal peptidases.


The pathology of leadership.

The pathology of leadership.


Surgeons and health on the endeavour.

Surgeons and health on the endeavour.


The full-thickness eyelid graft.

The full-thickness eyelid graft.


Clinical trial of a new antibiotic.

Clinical trial of a new antibiotic.


Guy Austin Lendon.

Guy Austin Lendon.


John Charters Kerridge.

John Charters Kerridge.


Alexander Roy McGregor.

Alexander Roy McGregor.


Nan Czulak.

Nan Czulak.


Superficial malignant melanoma. Clinicopathologic correlation.


The use of 5-fluorouracil cream to define suitable donor areas.


The Question Mark ear.

The Question Mark ear.


Lymphocyte transformation observed in sulfamylon agranulocytosis.


Subconjunctival antogenous dermal graft to prevent extrusion of orbital implant.


Volar approach for metacarpophalangeal joint capsulotomy.


Hyperbaric oxygenation of pedicle flaps without oxygen toxicity.


Surgical treatment of elephantiasis of male genitalia.


Multiple basal cell carcinomas arising in radiated burn scars. Case report.


Closure of large septal perforations with a labial-buccal flap.


Skin necrosis secondary to cryoglobulinemia. Case report.


Computer analysis of the electrocardiogram: evaluation of experience in a hospital heart station.


A rapid method for preparation of lymphocytes from whole blood for tissue typing.


Evaluation of a zone size chart for antibiotic susceptibility tests by disk diffusion.


Time-saver containers for tissue processing in electron microscopy.


Experience with induction of ovulation.

Experience with induction of ovulation.


A selected public health bibliography with annotations.


Effects of radiation on man.

Effects of radiation on man.


Yeast science today and tomorrow.

Yeast science today and tomorrow.


Preparation of a monospecific anti-Saccharomyces carlsbergensis serum for the biological quality control.


Cyto-immunological studies on Candida.

Cyto-immunological studies on Candida.


The validity of the isotope dilution method of measuring extracellular fluid volume after acute haemorrhage.


Historical survey of experimental thrombolysis.


Streptokinase in acute myocardial infarction: rationale and preliminary results of a combined therapeutic trial.


The rate of resolution of pulmonary embolism and its effects on early survival.


The present status of medical ultrasonic diagnostics.


A general practitioner study to evaluate the efficacy of 'Proctosedyl' ointment in the treatment of acute fissure-in-ano.


Life and death in Shakespeare's London.

Life and death in Shakespeare's London.


Deliberate delayed primary skin-grafting.


Dermal sinus and intraspinal dermoid and epidermoid cyst in children.


Mixed infections of Coxiella burnetii in restriciive host cells: an immunofluorescent study.


Sorption of water by spores, heat-killed spores, and vegetative cells.


Computer program for pattern recognition of electrocardiograms.


Inhibition of the induced synthesis of protocatechuate oxygenase by o-nitrobenzoic acid.


Treatment of basal cell carcinoma with 5-fluorouracil (5-FU) ointment.


The effects of 5-fluorouracil (5-FU) ointment in the treatment of neoplastic dermatoses.


Treatment of skin epitheliomas with 5-fluorouracil (5-FU) ointment. Influence of therapeutic design on recurrence of lesions.


Applications of fluorescent microscopy in diagnostic microbiology.


SI--Système International d'Unités.

SI--Système International d'Unités.


Application of the enzymatic double isotope dilution assay for the study of histamine in plasma.


Model for the determination of growth rate in Escherichia coli.


A comparative study of two long-acting antihistamines.


Evidence for the generality of linear cell growth.


Heat-sensitive early function in induced lambda Nsus lysogens. Mutations in gene N of lambda prevent killing of the host bacterium after infection. However, derepression of Nsus prophages in nonpermissive (pm(-)) bacteria results in death of the lysogens. When prophages in pm(-)(lambdaCItsA-Nsus) lysogens are derepressed by raising the temperature to 45 C, the cells remain viable as long as they are at 45 C. However, they cannot form colonies at 33 C unless they have been superinfected, at the high temperature, by lambdaCI(+)-Nsus phage which produces repressor at 45 C. A large fraction of these "rescued," heat-inducible lysogens are lysogenized by the superinfecting phage, but lysogenization is not required for rescue. In pm(-)(lambdaCItsA-Nsus) lysogens growing at 45 C, the rate of deoxyribonucleic acid (DNA) synthesis shows a characteristic increase after the temperature is lowered. This increased DNA synthesis, which is correlated with loss of rescue potential, does not occur as long as the cultures are maintained at 45 C.


Complement in synovial fluid.

Complement in synovial fluid.


Concentration of homovanillic acid in the ventricular fluid of patients with Parkinson's disease and other dyskinesias.


Thoracic duct fistulas in man.

Thoracic duct fistulas in man.


A study of Salmonella contamination in some Dublin poultry processing plants.


Studies on the toxicity of Alternaria mali.


Infection in goats owing to Serratia marcescens.


Disappearance rates and immunosuppression of intermittent intravenously administered prednisolone in rabbits and human beings.


A Warwickshire cattle plague association.


An evaluation of the interstitial nerve cells in the cerebellum.


Medicare progress and problems.

Medicare progress and problems.


Histometric study of age related changes in the thoracic aorta of Gallus gallus (Linnaeus, 1758).


Utilization of diacylglycerol species by cholinephosphotransferase, ethanolaminephosphotransferase and diacylglycerol acyltransferase in rat liver microsomes.


Management of the ureter in renal transplantation.


Syphilitic lymphadenitis.

Syphilitic lymphadenitis.


Folate and vitamin B12 in epilepsy.

Folate and vitamin B12 in epilepsy.


Bromhexine in chronic bronchitis.

Bromhexine in chronic bronchitis.


Survival rates of patients treated by home and hospital dialysis and cadaveric renal transplantation. Of 109 patients admitted to the renal failure programme of the Royal Free Hospital 80.7% were surviving after six years. For patients on home dialysis the four-year rate was 86.2%, more deaths occurring in women than in men. The short-term survival rate of all patients was high-namely, 96.3% at six months and 94.4% at 12 months. Hence large increases in the rate of acceptance of patients as well as successful transplantation are necessary to balance acceptance and loss from this programme.


Acute urinary tract infection in children.


Anencephaly: a review and interpretation in terms of modern experimental embryology.


Cardiac toxicity and psychotropic drugs.


Purification and comparison of the N-(5'-phosphoribosyl)anthranilic acid isomerase-indole-3-glycerol phosphate synthetase of tryptophan biosynthesis from three species of Enterobacteriaceae.


Cross-linkages in collagen. Demonstration of three different intermolecular bonds.


Regulation of gluconeogenesis in the guinea pig liver.


Studies on the alpha-methylglucoside permease of Escherichia coli. A two-step mechanism for the accumulation of alpha-methylglucoside 6-phosphate.


Immunochemistry of R lipopolysaccharides of Escherichia coli. Studies on R mutants with an incomplete core, derived from E. coli O8:K27.


Enhancement by the adjuvant, endotoxin, of an immune response induced in vitro.


Rate of antigen catabolism and immunogenicity of [131-I]BGG in mice. I. Action of mycobacterial adjuvants.


Dr. S. T. Achar.

Dr. S. T. Achar.


The induction of an immune response in vitro to a protein antigen (rabbit Fab'2).


Reminiscences of late Professor S.T. Achar.


Ultrastructure of the primary immune response. Development of 19S antibody-producing cells.


Identification of lachrymators.

Identification of lachrymators.


Something old.

Something old.


Psychophysiology and development of social attachment.


The effects of lithium on electroencephalogram, behavior and serum electrolytes.


A longitudinal investigation of adrenocortical function in acute schizophrenia.


Content of ATP in cultivated neurons and astrocytes exposed to balanced and potassium-rich media.


The subcommissural organ.

The subcommissural organ.


The mammalian pineal organ.

The mammalian pineal organ.


The neurosecretory cell of vertebrates.

The neurosecretory cell of vertebrates.


Substance P and its central effects.

Substance P and its central effects.


The neuronal connexions of the hypothalamic neurosecretory nuclei in mammals. A general survey.


Neurohormonal mechanisms controlling trophic hormone secretion of the anterior pituitary.


Microencapsulation.

Microencapsulation.


Kinetics and mechanisms of action of drugs on microorganisms. XI. Effect of erythromycin and its supposed antagonism with lincomycin on the microbial growth of Escherichia coli.


Indicator dilution studies in the small bowel of patients with cholera diarrhea. I. Jejunal flow--transit time--volume relationships.


The recognition and measurement of pulmonary emphysema.


Whitney Clair Porter, MD.

Whitney Clair Porter, MD.


Reuben J. Boesel, MD.

Reuben J. Boesel, MD.


Porcine salmonellosis. 3. Production of fibrinous colitis by intravenous injections of a mixture of viable cells of salmonella cholerae-suis and disintegrated cells of the same agent, or hemolytic Escherichia coli.


N-iodoacetyl-D-glucosamine, an inhibitor of growth and glycoside uptake in Escherichia coli. 1. Synthesis of N-iodoacetyl-d-glucosamine and its N-iodo[1,2-(14)C(2)]acetyl form has been achieved from the tetra-O-acetyl amino sugar and iodoacetic acid in the presence of dicyclohexylcarbodi-imide followed by catalytic deacetylation. 2. N-Iodoacetylglucosamine (up to 0.1mm) linearly inhibits uptake (up to 1min) of methyl alpha-d-glucoside by Escherichia coli ML308 and K12. Uptake of methyl beta-d-thiogalactoside and glycerol is also inhibited. 3. Growth of the organism (strain ML308) on glucose, succinate and glycerol is strongly inhibited by the iodoacetyl compound. The inhibition is relieved by N-acetylglucosamine. 4. The inhibitor has multiple effects, some of which are considered to be intracellular. 5. A separate transport pathway exists for N-acetylglucosamine by means of which the iodoacetyl analogue may enter the cell.


Glucose metabolism in mouse pancreatic islets. 1. Rates of glucose oxidation, lactate output and the intracellular concentration of glucose 6-phosphate were measured in mouse pancreatic islets incubated in vitro. 2. Glucose oxidation rate, measured as the formation of (14)CO(2) from [U-(14)C]glucose, was markedly dependent on extracellular glucose concentration. It was especially sensitive to glucose concentrations between 1 and 2mg/ml. Glucose oxidation was inhibited by mannoheptulose and glucosamine but not by phlorrhizin, 2-deoxyglucose or N-acetylglucosamine. Glucose oxidation was slightly stimulated by tolbutamide but was not significantly affected by adrenaline, diazoxide or absence of Ca(2+) (all of which may inhibit glucose-stimulated insulin release), by arginine or glucagon (which may stimulate insulin release) or by cycloheximide (which may inhibit insulin synthesis). 3. Rates of lactate formation were dependent on the extracellular glucose concentration and were decreased by glucosamine though not by mannoheptulose; tolbutamide increased the rate of lactate output. 4. Islet glucose 6-phosphate concentration was also markedly dependent on extracellular glucose concentration and was diminished by mannoheptulose or glucosamine; tolbutamide and glucagon were without significant effect. Mannose increased islet fructose 6-phosphate concentration but had little effect on islet glucose 6-phosphate concentration. Fructose increased islet glucose 6-phosphate concentration but to a much smaller extent than did glucose. 5. [1-(14)C]Mannose and [U-(14)C]fructose were also oxidized by islets but less rapidly than glucose. Conversion of [1-(14)C]mannose into [1-(14)C]glucose 6-phosphate or [1-(14)C]glucose could not be detected. It is concluded that metabolism of mannose is associated with poor equilibration between fructose 6-phosphate and glucose 6-phosphate. 6. These results are consistent with the idea that glucose utilization in mouse islets may be limited by the rate of glucose phosphorylation, that mannoheptulose and glucosamine may inhibit glucose phosphorylation and that effects of glucose on insulin release may be mediated through metabolism of the sugar.


The role of the phosphoenolpyruvate phosphotransferase system in the transport of N-acetyl-D-glucosamine by Escherichia coli. The properties of an N-acetyl-d-glucosamine-transport system have been studied by following the intracellular accumulation of methyl 2-acetamido-2-deoxy-alpha-d-[1-(14)C]glucoside by Escherichia coli. The same analogue was used to assay phosphoenolpyruvate phosphotransferase activity of toluene-treated cells. Transport and phosphorylation are induced by growth on d-glucosamine or N-acetyl-d-glucosamine. Mutants resistant to N-iodoacetyl-d-glucosamine are defective in the uptake and phosphorylation of the labelled glycoside.


Suturing technique for squint surgery.

Suturing technique for squint surgery.


An examination of the inhibitory effects of N-iodoacetylglucosamine on Escherichia coli and isolation of resistant mutants. 1. After incubation of Escherichia coli with N-iodo[1,2-(14)C(2)]acetylglucosamine, 95-99% of the (14)C taken up by whole cells is located in a cold-trichloroacetic acid-soluble fraction. Two major components of this fraction are S-carboxymethylcysteine and S-carboxymethylglutathione. The same compounds accumulate during incubation with iodo[(14)C]acetate but not with iodo[(14)C]acetamide. The amount of (14)C associated with a cold-trichloroacetic acid-insoluble fraction are comparable for all three alkylating agents. After incubation with iodo[(14)C]acetamide, 50% of the label bound to whole cells is recoverable in a cold-trichloroacetic acid-insoluble fraction. 2. Uptake and incorporation of (14)C from [U-(14)C]glycerol is blocked at an early stage by N-iodoacetylglucosamine. No specific inhibition of macromolecular synthesis could be demonstrated. 3. Mutants selected for resistance to iodoacetate are partially resistant to iodoacetate and N-iodoacetylglucosamine, but show no resistance to iodoacetamide. 4. Mutants selected for resistance to N-iodoacetylglucosamine are not resistant to iodoacetate or iodoacetamide, and are defective in their ability to grow on N-acetylglucosamine. Resistance to N-iodoacetylglucosamine is not absolute, and depends on the presence of glucose or certain other sugars; there is no resistance during growth on maltose, glycerol or succinate. 5. Absolute resistance can be achieved by selecting for a second mutation conferring resistance during growth on maltose; double mutants isolated by this procedure are unable to grow on N-acetylglucosamine and grow poorly on glucosamine. Resistant single mutants have a slightly diminished uptake of N-acetyl[1-(14)C]glucosamine, but in resistant double mutants the uptake of both [1-(14)C]glucosamine and N-acetyl[1-(14)C]glucosamine is severely diminished. 6. These observations are consistent with the presence of two permeases for N-acetylglucosamine, one that also permits uptake of glucosamine and another that allows entry of methyl 2-acetamido-2-deoxy-alpha-d-glucoside. N-Iodoacetylglucosamine can gain entry to the cell by both permeases.


What is the signal for chromatolysis?

What is the signal for chromatolysis?


Study on the immunological reactivity of mice with solid Ehrlich adenocarcinoma.


Study on immunological reactivity of mice treated with antigen from ascitic form of Ehrlich carcinoma.


The occupational therapist as consultant to the aged.


Ultrastructural synaptology of Clarke's column.


Ocular disease associated with blastomycosis in dogs.


Disseminated coccidioidomycosis in a dog.


Dermoid sinus in a Rhodesian Ridgeback dog.


A new method for the purification of initiation factor F2 in high yield, and an estimation of stoichiometry in the binding reaction.


Homoserine dehydrogenase of Rhodospirillum rubrum. II. Purification and characterization of the enzyme.


Changes of membrane proteins and their relation to deoxyribonucleic acid synthesis and cell division of Escherichia coli.


An introduction to the electron microscope.


The physiological behavior of enzyme I and heat-stable protein mutants of a bacterial phosphotransferase system.


Fine structure of polyphosphate granules in Micrococcus lysodeikticus.


Cholera in the new world.

Cholera in the new world.


Clinical trial of topical nitrofurazone, with or without hydrocortisone, in 252 children with skin infections.


Antibodies to species homologous tissue antigens in the rat. IV. Correlation of circulating antibodies with glomerulonephritis in rats sensitized with homologous tissue components.


Roderick Macdonald.

Roderick Macdonald.


"Psychiatrist, humanitarian, and scholar" James Woods Babcock, M.D.


Secondary structure of 5 S ribosomal RNA.


Biochemical determination of bacterial morphology and the geometry of cell division.


Dissociation of ribosomes on polypeptide chain termination and origin of single ribosomes.


Immunofluorescent localization of chorionic gonadotrophin in monolayer cultures of human trophoblast cells.


Non-suppressible insulin-like activity in relation to liver function and to disease.


First attempts of clinical application of a method for the quantitative interpretation of renogram with radiohippuran.


The occurrence of steroids and steroid metabolizing enzyme systems in invertebrates. A review.


The supporting role of community hospitals in a kidney transplant program.


The histological research on the rabbit aqueous out flow route.


Directories of American Roentgen Ray Society and American Radium Society.


Robert Drane, M.D., 1887-1970.

Robert Drane, M.D., 1887-1970.


An elution device for analytical-scale polyacrylamide gel electrophoresis.


Reduction and reoxidation of pepsinogen and pepsin.


Affinity labeling of myoglobin with mesoheme sulfuric anhydride.


Mesoheme sulfuric anhydride as a heme protein structure probe reaction with cytochrome b 562.


Equine antihapten antibody. Studies on the primary structure and conformation of equine immunoglobulins.


Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.


Interaction of kanchanomycin with nucleic acids. 3. Contrasts in the mechanisms of inhibition of ribonucleic acid and deoxyribonucleic acid polymerase reactions.


Purification and some properties of thiolase from Escherichia coli.


Radiation effects on an allosteric enzyme with two catalytic activities. X-ray inactivation of the threonine-sensitive asparto-kinase-homoserine dehydrogenase from Escherichia coli.


World problems in viral vaccines.

World problems in viral vaccines.


Haemodynamic studies with peruvoside in human congestive heart failure. The immediate haemodynamic effects of peruvoside, a cardiac glycoside obtained from the Indian plant Thevetia neriifolia Juss, were studied in six patients with congestive heart failure. The drug was found to have an immediate and powerful positive inotropic and negative chronotropic effect, like ouabain, on the failing human heart. Oral peruvoside was also effective in the treatment of congestive heart failure when used on a short-term as well as a long-term basis. It therefore seems that peruvoside is a useful cardiac glycoside in the management of congestive heart failure in man as a quick-acting intravenous preparation. It is equally effective when used orally.


Treatment of menorrhagia with tranexamic acid. A double-blind trial. In a double-blind trial tranexamic acid (Cyclokapron) 1 g. four times a day for the first four days of menstruation, significantly decreased menstrual blood loss in women with menorrhagia for which no organic cause had been found. No difference in side-effects was noted between the active and placebo treatment.


Pyrimidine metabolism in microorganisms.


Postoperative leg vein thrombosis.

Postoperative leg vein thrombosis.


Reception of Röntgen's discovery in Britain and U.S.A.


Human liver transplantation.

Human liver transplantation.


John Ridgway, M.B., Ch.B.

John Ridgway, M.B., Ch.B.


Duncan Wood, M.B., B.S., F.R.C.S.

Duncan Wood, M.B., B.S., F.R.C.S.


Cryofixation. Control of ocular shape and movement by cryoadhesion.


Basic cytogenetics.

Basic cytogenetics.


James McCallmont, Delaware physician during the revolutionary period, 1776-1787.


Specific dissociation of ribosomes from Saccharomyces cerevisiae by a protein factor.


Wheat in human nutrition.

Wheat in human nutrition.


The William Osler medal essay. Aneurysm and physiologic surgery.


Freeze-etching.

Freeze-etching.


Sliding skin grafts in the treatment of anal fissures.


Prolactin, the Golgi apparatus, and milk secretion. Brief interpretive review.


Phenotyping milk proteins. A review.

Phenotyping milk proteins. A review.


Review of literature on diseases of the colon and rectum. March-April, 1969--compiled by Robert J. Rowe, M.D., M.S. and Elinor C. Reinmiller, B.A., B.S. in L.S., M.A.


Differences between calf and adult bovine rennet.


Salmonella typhimurium infection in mice treated with niridazole.


Hypersensitivity in young piglets: its relation to the pathogenesis of Escherichia coli disease.


The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine origin.


The effect of cell-free fluids prepared from cultures of human and animal enteropathogenic strains of Escherichia coli on ligated intestinal segments of rabbits and pigs.


In-vitro sensitivity of Shigella sonnei to trimethoprim and sulphamethoxazole.


Evolution of selection methods for admission to medical school. I. Matriculation grades, entrance examination and personal interview as predictors of achievement in medical school.


Electron-microscope illustrations of division in Mycobacterium leprae.


Comparison of a microbiological and a spectrophotometric method for the determination of nicotinic acid in fresh meat.


A review of the use of prosthetic materials in tendon surgery.


Comparison of World War II, Korean, and Vietnamese prisoners of war.


Psychological effects of prisoner-of-war experiences.


The columnar epithelial cell of the small intestine: digestion and transport. (First of three parts).


Innervation of transplanted kidney.

Innervation of transplanted kidney.


Implantable nuclear-powered cardiac pacemakers.


The health of Stilwell's troops.

The health of Stilwell's troops.


Factor necessary for ribosomal RNA synthesis.


Antigen binding cells in tolerant animals.


Coupled transport of sodium and organic solutes.


Clinical value of the electrogram of the conduction system.


Pacemakers and their arrhythmias.

Pacemakers and their arrhythmias.


Some physical and hydrodynamic properties of human FSH and LH.


The effect of constriction on the conduction of the action potential in the sciatic nerve.


The nonreversed saphenous vein bypass for femoral-popliteal occlusive disease.


A new method of renal artery reconstruction.


The hemolytic uremic syndrome.

The hemolytic uremic syndrome.


Technique of femorotibial bypass.

Technique of femorotibial bypass.


Hyperlipidemia and pulmonary fat embolism in dogs with E. coli bacteremia.


Edwin H. Ellison, M.D.

Edwin H. Ellison, M.D.


Experience on the use of homologous cartilage in nasal surgery.


President's speech.

President's speech.


Mediastinal-reticulo-lympho-sarcoma operated and cured.


Diagnostic biochemical methods in pancreatic disease.


Serum albumin.

Serum albumin.


Fluorometry and phosphorimetry in clinical chemistry.


Relationships between renal vascular volume and blood flow resistance.


Effect of ambient temperature on gastric secretion and food intake in the rat.


The military influence upon Freud's dynamic psychiatry.


Van Gieson, a visionary of psychiatric research.


Amitriptyline and trimipramine in neurotic depressed outpatients: a collaborative study.


Clarence B. Farrar, S.M., A.B., M.D., D.Sc., L.L.D., F.R.C.P. (C).


Critique of studies of lithium salts in the treatment of mania.


The multiplication of Mycobacterium leprae in denervated mouse feet.


Analgesic effects of alpha-d-2-acetoxy-1, 2-diphenyl-3-methyl-4-pyrrolidinobutane hydrochloride (compound 31518).


Comparative pharmacological investigations on naphthypramide and some anti-inflammatory and skeletal muscle relaxant agents.


Phosphorylation of dephosphorylated tRNA and oligonucleotides by polynucleotide kinase.


Further studies on amino acid acceptance and physical properties of tRNA-phe-yeast.


Effect of 5'-substitution on the template activity of oligo-nucleotides for the binding of valine and alanine tRNA to ribosomes.


An apparent identity of 5-S RNA from free and membrane-bound reticulocyte ribosomes.


Erroneous transcription of mitochondrial DNA by RNA polymerase from Escherichia coli.


The binding of Escherichia coli RNA polymerase to rabbit thymus deoxyribonucleoprotein.


Replication of viral nucleic acids. I. Enzyme-RNA complexes involved in the synthesis of Q-beta RNA.


Effect of secondary structure on the degradation of ribosomal RNA.


Peptidyl-tRNA hydrolase and protein chain termination.


Effect of acridines on DNA synthesis in vitro: base composition of product and inhibition in the presence of different primers.


Abnormal growth and tissue tension in paralytic lesions in man.


Skin transplantation and the types of lymphocytes involved in rejection.


The proliferation of transplanted haematopoietic cells derived from bone marrow and foetal liver.


The Medical Association of Georgia. 1970 yearbook.


Replicate inoculation of multi-chambered trays.


Fetal factors influencing the volume and composition of liquor amnii.


Interaction of sodium gamma-hydroxybutyrate with catecholamines in rat heart and adipose tissue.


Trauma and skin cancer.

Trauma and skin cancer.


Recovery from radiation injury with and without bone marrow transplantation: effects of splenectomy.


Chronic active hepatitis: possible adverse effect of exploratory laparotomy.


Current status: treatment of acute leukemia.


Evaluation of local antibiotic instillation in extremity wounds.


Renal allograft rejection simulated by arterial stenosis.


Infection: a cause of acute peptic ulceration in hypersecreting animals.


Technique for permanent implantation of atrial pacemaker.


Color radiography and the substraction technique.


Stereoencephaloscopy. (A new microsurgical examination and intervention by means of the stereotaxic endoscopy).


Serum enzymes during cardiac allograft rejection.


Pentazocine in the relief of acute cardiac pain.


Quantitative histological studies on the aged human brain. I. Senile plaques and neurofibrillary tangles in "normal" patients.


Quantitative histological studies on the aged human brain. II. Senile plaques and neurofibrillary tangles in senile dementia (with an appendix on their occurrence in cases of carcinoma).


Determination of trace amounts of contaminants in water by isotope dilution gas chromatography.


Ulcerative colitis.

Ulcerative colitis.


The vagus, the bile, and gallstones.

The vagus, the bile, and gallstones.


Immune response at high altitude.

Immune response at high altitude.


Amodal totalization and the "gestalt-error".


Construction of a new aortic valve. A new procedure and a preliminary report.


Increase of dermal blood flow after sympathectomy as measured by radioactive sodium uptake.


Structure and function of the median eminence.


Electron microscopic changes following canine cardiac allografts: the effects of irradiation.


Protein-bound phosphorylthreonine in some tissues and organisms.


Studies on phospholipase A and its zymogen from porcine pancreas. II. The assignment of the position of the six disulfide bridges.


Formation of aromatic amino acid pools in Escherichia coli K-12. Phenylalanine, tyrosine, and tryptophan were taken up into cells of Escherichia coli K-12 by a general aromatic transport system. Apparent Michaelis constants for the three amino acids were 4.7 x 10(-7), 5.7 x 10(-7), and 4.0 x 10(-7)m, respectively. High concentrations (> 0.1 mm) of histidine, leucine, methionine, alanine, cysteine, and aspartic acid also had an affinity for this system. Mutants lacking the general aromatic transport system were resistant to p-fluorophenylalanine, beta-2-thienylalanine, and 5-methyltryptophan. They mapped at a locus, aroP, between leu and pan on the chromosome, being 30% cotransducible with leu and 43% cotransducible with pan. Phenylalanine, tyrosine, and tryptophan were also transported by three specific transport systems. The apparent Michaelis constants of these systems were 2.0 x 10(-6), 2.2 x 10(-6), and 3.0 x 10(-6)m, respectively. An external energy source, such as glucose, was not required for activity of either general or specific aromatic transport systems. Azide and 2,4-dinitrophenol, however, inhibited all aromatic transport, indicating that energy production is necessary. Between 80 and 90% of the trichloroacetic acid-soluble pool formed from a particular exogenous aromatic amino acid was generated by the general aromatic transport system. This contribution was abolished when uptake was inhibited by competition by the other aromatic amino acids or by mutation in aroP. Incorporation of the former amino acid into protein was not affected by the reduction in its pool size, indicating that the general aromatic transport system is not essential for the supply of external aromatic amino acids to protein synthesis.


Purification and some properties of two boticins. Two bacteriocins (boticins) were elaborated without induction by strain S5, a nontoxigenic variant of Clostridium botulinum type E. After separation of the two active entities by gel filtration on Sephadex G-50, a large particle with boticin activity was isolated by density gradient ultracentrifugation, and a small soluble boticin was purified by continuous curtain electrophoresis and chromatography on sulfoethyl-Sephadex. Large and small boticins were purified 200- and 3,000-fold, respectively, with yields of 50% or more. The small boticin, a basic substance with molecular weight under 30,000, was the predominant species; the large boticin, a negatively charged particle with particle weight greater than 40 x 10(6), represented less than 20% of the total activity. Both purified boticins were resistant to heat and were attacked by proteolytic enzymes, but the large boticin was less thermostable and less sensitive to proteolytic enzymes than was the smaller variety. The activity of the large boticin was not reduced by treatment with urea or deoxyribonuclease. Both boticins exhibited sporostatic and bactericidal activities for C. botulinum type E, strain 070. A suspension of type E strain 070 vegetative cells was rendered nonviable within 9 min by the small boticin. The lethal action of this bacteriocin was not reversed by trypsin.


A case of pulmonary infection with two mycobacterial serotypes.


Sensitivity of Streptococcus pyogenes types to tetracycline and other antibiotics.


Computer study of thyroid diseases. I. Methodology.


Mutations affecting iron transport in Escherichia coli. A mutant of Escherichia coli K-12 unable to form an essential component of the enterochelin-dependent iron transport system has been isolated. This strain carries a mutation in a gene designated fep, mapping close to two genes, entA and entD, concerned with enterochelin synthesis. Strain AN102, which carries the fep(-) allele, accumulates large quantities of enterochelin and gives a growth response to sodium citrate. The cytochrome b(1) and total iron content, and the measurement of the uptake of (55)Fe(3+), indicate an impairment of the enterochelin-dependent iron transport system. The growth response to sodium citrate is related to the presence, in strain AN102, of an inducible citrate-dependent iron transport system.


Specific labeling and physical characterization of R-factor deoxyribonucleic acid in Escherichia coli. The molecular nature of R-factor deoxyribonucleic acid (DNA) was examined in Escherichia coli by using a method for the specific labeling of the derepressed R factor, R1, in a female cell after conjugation. Sixty minutes after mating, the R factor was isolated as a single molecule with a molecular weight of 65 x 10(6) daltons. This single molecular species sedimented as either a covalently closed molecule or a "nicked" circle. When the single R-factor component was centrifuged in a CsCl density gradient, only a single homogeneous species with a buoyant density of 1.711 g/cm(3) was observed. R-factor DNA was also isolated directly from exponentially growing cells of E. coli as a covalently closed single molecular species comprising about 1% of the total cellular DNA. Previous studies in Proteus show that R1 factor DNA components of buoyant density 1.709, 1.711, and 1.716 g/cm(3) can be identified as distinct replicons. It is suggested that the single molecule of R1 observed in E. coli is most simply explained as a composite structure resulting from a recombinational assemblage of a 1.709 and 1.716 g/cm(3) replicon.


Effects of ribonucleosides on thymidine incorporation: selective reversal of the inhibition of deoxyribonucleic acid synthesis in thymineless auxotrophs of Escherichia coli. Addition of certain ribonucleosides to exponentially growing cultures of Escherichia coli increased the extent of thymidine incorporation. The prolonged uptake of thymidine was correlative with the ability of these ribonucleosides to prevent the degradation of thymidine. In addition to protecting thymidine, uridine reversed partially (70 to 80%) the inhibition of deoxyribonucleic acid (DNA) synthesis in thymineless auxotrophs by cytosine arabinoside, hydroxyurea, and nalidixic acid. This reversal was selective for auxotrophic strains since no reversal of inhibition by uridine was observed in any of the prototrophic strains examined. In the presence of uridine, the rapid assimilation of thymidine by prototrophic and auxotrophic strains was prevented and the rate of DNA synthesis became a function of the available exogenous thymidine. Under these conditions, prototrophic strains accumulated equivalent amounts of thymidine into the acid-soluble (pool) and acid-insoluble (DNA) cell fractions. In contrast, 95 to 98% of the thymidine taken up by auxotrophs was found in the acid-insoluble (DNA) cell fraction. The results suggest that different mechanisms for DNA synthesis exist in auxotrophs and prototrophs. Based on these observed differences, some possible mechanisms for the selective reversal of the inhibition of DNA synthesis in auxotrophs are discussed.


Studies on resistance transfer factor deoxyribonucleic acid in Escherichia coli. A variant of the derepressed R factor, R1, which does not contain any of the drug resistance markers, and represents, in large part, the resistance transfer factor (RTF) was studied in Escherichia coli. RTF deoxyribonucleic acid (DNA) was specifically labeled in a female cell after conjugation. Physical characterization of the molecule showed that RTF possessed an average molecular weight of 50 x 10(6) daltons and a buoyant density of 1.709 g/cm(3). By comparison to R1, we calculate that the region of DNA carrying the drug resistance genes is therefore about 20% of the R1 molecule and has a buoyant density of approximately 1.716 g/cm(3). These results support the hypothesis that the single species of R-factor DNA observed in E. coli represents a composite of the 1.709 and 1.716 g/cm(3) replicons seen in Proteus.


Computer study of thyroid diseases. II. Results.


Recombination-deficient mutants of colicinogenic Salmonella typhimurium detected by their failure to produce colicin. Survivors of nitrosoguanidine-treated cultures of a colicinogenic strain of Salmonella typhimurium were tested for spontaneous production of colicin E1. Of about 1,000 colonies tested, 13 produced no (or very narrow) colicin zones. Four of these isolates proved to be more sensitive to ultraviolet (UV) light, X rays, and methyl methane sulfonate than the parent strain and did not show enhanced production of colicin when treated with mitomycin C (which acts as an inducer on wild-type cells). Further studies showed that these isolates were of two classes. Three mutants were extremely sensitive to UV, failed to show spontaneous release of two temperate phages, and were infertile as recipients in transduction or in an Hfr cross although they accepted an F' factor normally. These independently isolated mutants were inferred to be recombination-deficient; one of them had the additional property of increased spontaneous mutability at two loci. The other colicin-nonreleasing isolate was only moderately sensitive to UV, showed enhanced spontaneous release of two temperate phages, and was of approximately normal fertility as a recipient in transduction or conjugation.


Circular R-factor molecules controlling penicillinase synthesis, replicating in Escherichia coli under either relaxed or stringent control. Two infectious drug-resistance (R) factors, R28K and R6K, each conferring resistance to a number of penicillins by the synthesis of a penicillinase, were transferred to Proteus mirabilis PM1 and Escherichia coli RC85 host strains. Deoxyribonucleic acid (DNA) extracted from these strains was separated by density-gradient centrifugation and subjected to electron microscopy by use of a modification of the protein-monolayer diffusion technique. Analytical density-gradient centrifugation of the purified DNA from PM1 strains showed, in addition to the major peak at a density of 1.698 g/cm(3) characteristic of Proteus chromosomal DNA, a single satellite band at a density of 1.710 g/cm(3) [guanine plus cytosine (GC) base ratio 50%] for R28K and at 1.704 g/cm(3) (GC base ratio 45%) for R6K. Direct CsCl density-gradient centrifugation of crude lysates of the E. coli (R28K)(+) strain in the presence of ethidium bromide gave rise to a sedimentation profile with a single satellite peak containing covalently closed circular (CCC) DNA molecules with a mean contour length of 21.4 mum [44 x 10(6) atomic mass units (AMU)], although a minority was 13.6 mum in length. From the size of the major class, it was estimated that there were two to three copies of the R28K factor present as CCC molecules per chromosome at various phases of cellular growth. Similar studies of the E. coli (R6K)(+) lysates showed two satellite peaks; peak I contained mostly CCC molecules of contour length 12.8 mum (26 x 10(6) AMU), and peak II, intermediate to peak I and the chromosomal peak, contained CCC molecules of a similar size, together with about equal numbers of catenated molecules, mostly dimers consisting of two interlocked monomers of 12.8 mum. A smaller number (ca. 0.1%) of higher catenanes was also seen. The number of CCC copies of the R6K factor per chromosome present in peak I was calculated as 13 at logarithmic phase and 38 at stationary phase. In peak II, a constant ratio of about one catenated dimer per chromosome was found at all phases of growth. Penicillinase assays of cultures at different phases of growth showed a correlation between the estimated number of R-factor copies present as CCC molecules and the enzyme activity per cell for both R28K and R6K.


Isolation, characterization, and genetic analysis of mutator genes in Escherichia coli B and K-12. Twenty-one Mut mutants were obtained from Escherichia coli B (B/UV) and K-12 (JC355) after treatment with mutagens. These Mut strains are characterized by rates of mutation to streptomycin resistance and T-phase resistance which are significantly higher than the parental (Mut(+)) rates. Mutator genes in 12 strains have been mapped at three locations on the E. coli chromosome: one close to the leu locus; five close to the purA locus; and six close to cysC. In addition, eight mutator strains derived from E. coli B/UV are still unmapped. Some effort was made to deduce the mode of action of the mutator genes. These isolates have been examined for possible defects in deoxyribonucleic acid repair mechanisms (dark repair of ultraviolet damage, host-cell reactivation, recombination ability, repair of mitomycin C damage). By using transductional analysis, it was found that the ultraviolet sensitivity of NTG119 and its mutator property results from two separate but closely linked mutations. PurA(+) transductants that receive mut from NTG119 or NTG35 are all more sensitive to mitomycin C than is the PurA recipient. Unless transduction selects for sensitivity, a probable interpretation is that defective repair of mitomycin C-induced damage is related to the mode of action of mut in these transductants and the donor. Abnormal purine synthesis may be involved in the mutability of some strains with cotransduction of the mutator properly and purA (100% cotransduction for NTG119). Three mutators are recombination-deficient and may have a defective step in recombination repair. One maps near three rec genes close to cysC.


Cytological studies of deoxyribonucleic acid replication in Escherichia coli 15T-: replication at slow growth rates and after a shift-up into rich medium. We examined the gross nuclear morphology of Escherichia coli 15T(-) grown in different media with doubling times ranging from 22 to 270 min. In slowly growing cells, deoxyribonucleic acid synthesis was measured by autoradiography and shown to occur with greatest probability during the first two-thirds of the division cycle. In such cells, segregation occurred later, at the end of the division cycle rather than at the end of deoxyribonucleic acid replication. Nuclear regions in L-broth cells (22-min doubling time) cannot correspond to separate chromosomes but probably represent regions of replication activity. Segregation of template nucleotide strands was measured after a shift-up from proline M9 or glucose M9 media into L broth. A model is presented to account for the pattern of segregation observed.


Ultrastructure of deoxyribonucleic acid-membrane associations in Escherichia coli. Areas of contact between deoxyribonucleic acid (DNA) and intracytoplasmic membrane are frequently seen in the "extra" membrane-forming strain Escherichia coli 0111a(1). By examination of serial sections, it has been estimated that these DNA-membrane associations occur in at least 60% of the extra membrane-containing cells. Most of the DNA masses contained only one contact area. Several cells in which the DNA had been stretched revealed individual fibers connecting to the membrane, suggesting a firm attachment of DNA to membrane. The areas of membrane associated with DNA fibers were usually between 100 and 500 nm in diameter, although some smaller areas were seen. Electron microscopic autoradiography of cells in which the replication forks were labeled showed grains over 24% of the profiles containing a contact area, whereas there were grains over only 16% of the profiles without a contact area. Data from autoradiographs of cells in which the label was "chased" away from the replication fork showed the reverse labeling pattern. These data indicate that the areas of contact between DNA and intracytoplasmic membranes seen in electron micrographs contain the DNA replication forks.