Bacteriophage T4 inhibits colicin E2-induced degradation of Escherichia coli deoxyribonucleic acid. I. Protein synthesis-dependent inhibition. The deoxyribonucleic acid (DNA) of Escherichia coli B is converted by colicin E2 to products soluble in cold trichloroacetic acid; we show that this DNA degradation (hereafter termed solubilization) is subject to inhibition by infection with bacteriophage T4. At least two modes of inhibition may be differentiated on the basis of their sensitivity to chloramphenicol. The following observations on the inhibition of E2 by phage T4 in the absence of chloramphenicol are described: (i) Simultaneous addition to E. coli B of E2 and a phage mutated in genes 42, 46, and 47 results in a virtually complete block of the DNA solubilization normally induced by E2; the mutation in gene 42 prevents phage DNA synthesis, and the mutations in genes 46 and 47 block a late stage of phage-induced solubilization of host DNA. (ii) This triple mutant inhibits equally well when added at any time during the E2-induced solubilization. (iii) Simultaneous addition to E. coli B of E2 and a phage mutated only in gene 42 results in extensive DNA solubilization, but the amount of residual acid-insoluble DNA (20 to 25%) is more characteristic of phage infection than of E2 addition (5% or less). (iv) denA mutants of phage T4 are blocked in an early stage (endonuclease II) of degradation of host DNA; when E2 and a phage mutated in both genes 42 and denA are added to E. coli B, extensive solubilization of DNA occurs with a pattern identical to that observed upon simultaneous addition of E2 and the gene 42 mutant. (v) However, delaying E2 addition for 10 min after infection by this double mutant allows the phage to develop considerable inhibition of E2. (vi) Adsorption of E2 to E. coli B is not impaired by infection with phage mutated in genes 42, 46, and 47. In the presence of chloramphenicol, the inhibition of E2 by the triple-mutant (genes 42, 46, and 47) still occurs, but to a lesser extent.


Bacteriophage particles with endo-glycosidase activity. Some Escherichia coli K bacteriophage particles, capable of interacting specifically with bacterial polysaccharide capsules, carry an endo-glycosidase activity, probably located in the spikes.


Superinfection exclusion by P22 prophage and the replication complex. Wild-type sie(+) P22 prophage converted Salmonella typhimurium lysogens to exclude deoxyribonucleic acid (DNA) injected by superinfecting phage. DNA from a P22 superinfecting virulent phage associated with the replication complex in a sie(-) lysogen but not in a sie(+) lysogen.


Criteria and techniques for the diagnosis of gonorrhea.


Vinblastine treatment of malignant lymphomas.


Introductory address in memory of Dr. Václav Jelínek.


Absence of primary immune response in acute bovine lymphocytic leukemia. I. Occurrence of natural antibodies against E. coli.


Radiographic determination of the prevalence of selected dental anomalies in children.


Current concepts in the management of lymphoma.


Clifford Joseph Barborka: 1894-1971.

Clifford Joseph Barborka: 1894-1971.


William H. Haines: 1898-1970.

William H. Haines: 1898-1970.


The associative exercise. (Audiemus et Speremus). (Bertram D. Lewin).


"Farewell to a friend" (Bertram D. Lewin).


Our visiting professor (Bertram D. Lewin).


Dr. Lewin, tutor and friend.

Dr. Lewin, tutor and friend.


Dr. Lewin and the psychologists.

Dr. Lewin and the psychologists.


Dr. Bertram D. Lewin. 1896-1971.

Dr. Bertram D. Lewin. 1896-1971.


Bertram D. Lewin, M.D.

Bertram D. Lewin, M.D.


Always an educator (Bertram D. Lewin).

Always an educator (Bertram D. Lewin).


Papers on psychopharmacological research from the German literature.


Research in Yugoslavia.

Research in Yugoslavia.


Hypochromism.

Hypochromism.


The coupling between energy-yielding and energy-utilizing reactions in mitochondria.


Recovery from x-ray and nitrogen mustard inactivation of Escherichia coli by 2,4-dinitrophenol treatment.


L-asparaginase induced immunosuppression: inhibition of bone marrow derived antibody precursor cells. The cooperation between bone marrow and thymus cells in restoring the hemolytic antibody response to sheep erythrocytes in immunosuppressed recipients was markedly inhibited when donor mice were treated with L-asparaginase, a known inhibitor of lymphocyte function. The marrow cell population was shown to be a major target for the immunosuppressive activity of asparaginase, since thymus cells from enzyme-treated animals interacted with marrow cells from normal animals to generate immunocompetent cells.


Chemosterilant action of anthramycin: a proposed mechanism. The activity of anthramycin and structurally related analogs as chemosterilants of the housefly, Musca domestica L., correlates closely with the action of these compounds as inhibitors of Escherichia coli RNA polymerase. Since inhibition of RNA polymerase by anthramycin reflects binding of this antibiotic to the DNA primer required for enzyme activity, we propose that the interaction of anthramycin with DNA may also account for its action as a chemosterilant.


The replication of bacteriophage phi-X174 in a mutant of Escherichia coli strain C. I. Characterization of the physiological defect in viral reproduction.


The replication of bacteriophage phi-X174 in a mutant of Escherichia coli strain C. II. Evidence that the C-SM17ts mutation is not at the rep locus.


Replication of bacteriophage M13. VI. Attachment of M13 DNA to a fast-sedimenting host cell component.


Lysis defective mutants of bacteriophage lambda: genetics and physiology of S cistron mutants.


Formation of hybrid particles in RNA phages. I. Hybrid particles between RNA phages SP (IVa) and FI(IVb).


Lambdoid prophages modifying the kinetics of host cell division.


Nutritional status of infants and pre-school children: a review of surveys since 1960.


The uniqueness of human milk.

The uniqueness of human milk.


Needle with wire loop for drawing sutures through tissue.


Johannes Kepler on the eye and vision.

Johannes Kepler on the eye and vision.


Electron microscopy of posterior polymorphous degeneration.


Histopathologic findings in corneas following lens extraction and results of keratoplasty for aphakic bullous keratopathy.


Management of flail chest injury: a review of 75 cases.


The response of the reticuloendothelial system to skin transplantation. Phagocytic indices and weights of reticuloendothelial organs of mice bearing primary skin isografts and allografts.


The response of the reticuloendothelial system to skin transplantation. The cellular basis of hepatosplenomegaly in mice bearing skin grafts and its significance.


Altered ribosomes after inhibition of Escherichia coli by rifampicin. Addition of rifampicin to growing cells of Escherichia coli affected the ribosomes. The polyribosomes first decayed to 70S ribosomes. These later dissociated to particles distinct from ribosomal subunits. The altered ribosomes sedimented more slowly than the corresponding subunits and had lost some protein; their ribosomal RNA was intact, but they were more susceptible to degradation by ribonuclease than normal ribosomes. The addition of rifampicin to preparations of lysed cells caused no detectable changes in the ribosome fraction.


Competitive interaction of Escherichia coli leucyl-transfer-ribonucleic acids with the enzyme-leucyladenylate complex.


Gordon Michael Wyant.

Gordon Michael Wyant.


Red blood cell survival after mannitol infusion: a clinical study in human volunteers using Cr 51 .


Patients' attitudes toward ECT--not the 'shocker' we think?


Psychiatry in Vienna today.

Psychiatry in Vienna today.


Oscilloscopic and recent other methods of implanted pacemaker follow-up.


Surgical management of extracranial vascular disease.


Studies on transplantation immunity. 1. The nature of the response to allogeneic  51 Cr-labeled lymphoid cells and its relationship to homograft immunity.


Separate spleen cell populations synthesizing bacteriolytic versus agglutinating antibody in mice immunized with E. coli somatic antigen.


The chicken lacrimal gland, gland of Harder, caecal tonsil, and accessory spleens as sources of antibody-producing cells.


Hydroxyurea and cell-cycle kinetics of cultured antibody-forming cells.


Rosette-forming cells in the unimmunized mouse: morphological studies with phase contrast and electron microscopy.


Inhibition of macrophage aggregation in vitro by actinomycin D and puromycin.


Evidence for thymus-independent humoral antibody production in mice against polyvinylpyrrolidone and E. coli lipopolysaccharide.


In vitro studies of "antigenic competition". I. The comparative responses of normal and "immune" lymphoid cell populations.


Pierre A. Piorry (1794-1879), the French "master of percussion".


Intravenous diazepam in the treatment of prolonged seizure activity in neonates and infants.


The effect of methylphenidate on behavior of three school children: a pilot investigation.


A third ascending hypothalamopetal pathway.


Computer applications in the hospital pharmacy.


A survey of the literature on metallic surgical implants.


Historical paper on the fracture of the carpal extremity of the radius (1814).


Heat survivor curves of food-borne bacteria suspended in commercially sterilized whole milk. I. Salmonellae.


Pure and mixed continuous culture of two rumen anaerobes.


Memorials. William Earl Petersen 1892-1971.


Dietary urea for dairy cattle. I. Relationship to luteal function.


Memorials. John Albert Nelson 1890-1971.


Nasal and intestinal carriage of antibiotic resistant bacteria by patients on admission to hospital.


Medicine in the Union Armies.

Medicine in the Union Armies.


Official roster of affiliate societies.

Official roster of affiliate societies.


A proposed scale for the determination of maternal feeling.


Three-dimensional structure identified from single sections. Errors in the identification of objects have been made frequently because of faulty interpretation of their flat images or sections. Such errors are perpetuated through a strange psychological chain reaction. 1) The first author who observes sections of an object, although presenting faithful pictures of sections of it, identifies its shape incorrectly. Such an initial mistake may be due to identification of the flat image with the object itself or to failure to consider the shape of the image as a function of the shape of the real object and the angle and level of cutting. 2) The first author describes verbally his personal concept of the object. 3) The reader reads the text of a publication before or without examining the pictorial evidence. 4) The reader's belief in the authority of the printed word immunizes him against critical evaluation of the evidence. 5) The reader regards a published opinion as a "fact." 6) A mechanism of suppression of new evidence is set up in the brain of the reader in favor of previously published, verbalized concepts. His brain unconsciously rejects new concepts that might arise intracerebrally. The printed word supersedes visual evidence. 7) A secondary mechanism parallels steps 3 to 6. An observer notices an unfamiliar figure in the microscope. Following established procedures for scientific investigations, he refrains from forming an opinion about his observation. Instead he rushes to the library searching for similar, published observations. If he finds a previous report about a similar shape, he accepts it as a "fact" and he "confirms" the previously printed viewpoint. Let me interject a personal experience concerning this mechanism. I investigated the arrangement of blood vessels in the human liver by means of corrosion preparations. The work was divided between an assistant and me. He was assigned the task of preparing drawings of two specimens. In his drawings he showed a structure which was absent from the specimens but had been described in a previous publication. This article shows simple ways for correct initial identification of shape and structure and for the breaking of the reverberating circuit of erroneous thinking.


Heterotopic auxiliary liver transplantation in rats. Use of the infrahepatic vena cava as the efferent vessel.


Long-term observations of skin grafts between chimeric cattle twins.


Lymphocyte monitoring by blood lymphocyte counts and RNA-DNA determination in patients with a renal allograft.


Relationship of the immunosuppressive potency of antilymphocyte globulin to three in vitro tests. Close correlation with rosette inhibition.


Accelerated rejection of allogeneic skin grafts in the mouse after injections of histocompatibility antigens solubilized by Triton, butanol, and papain.


Antiglobin to cultured human lymphocytes does not bind to human allograft glomerular basement membrane.


Inhibition of the mixed leukocyte reaction as an assay for enhancing alloantiserum.


Thyroid transplantation. An alternative to skin grafting.


Tooth transplantation in the mouse. 3. The role of minor (non-H-2) histocompatibility loci in tooth germ transplantation.


A microassay for the detection of murine H-2 antigens.


Presensitization to transplants detected by cellular immunity tests.


Renal heterotransplant rejection. A model for separation of humoral and cellular mechanisms.


Modification of response to histocompatibility antigens with chemotherapeutic agents.


Multi-institutional use of a mobile kidney preservation unit.


Male-specific graft rejection and the H-2 locus.


Induction of tumors in neonatally thymectomized mice.


Vibrio infection in tropical fish in a freshwater aquarium.


Augustus Volney Waller on blood vessels and inflammation. II.


A two-filament system and interaction of heavy meromyosin (HMM) with thin filaments in smooth muscle.


Eugene Lindsay Opie, MD, 1873-1971.

Eugene Lindsay Opie, MD, 1873-1971.


New patterns of immunoglobulin deposition in the lesions of malignant nephrosclerosis, with special reference to IgE. Localization of immunoglobulins (including IgE), complement and fibrinogen and the morphologic alterations in the kidneys of 10 patients with malignant nephrosclerosis were investigated. Thirteen kidneys with benign nephrosclerosis and 5 normal ones were also studied. In contrast to a previous series of patients with malignant nephrosclerosis, the number of necrotic arterioles and the deposition of IgG and complement in the renal arterioles, were reduced in a parallel fashion. These differences seem to reflect modern treatment and support the hypothesis that IgG and complement play a role in the pathogenesis of the arteriolar necrosis in the disease. IgE was deposited massively in many renal arterioles of 9 patients with malignant nephrosclerosis. The role of reaginic antibody in the pathogenesis of the disease is not clear since IgE was also focally present in some arterioles of 4 normal kidneys and of 8 kidneys with benign nephrosclerosis.


Lymphocytes and hemic stem cells.

Lymphocytes and hemic stem cells.


Determination of bone marrow stem cell differentiation by stromal hemopoietic inductive microenvironments (HIM).


Regulatory mechanisms acting on hemopoietic stem cells. Some clinical implications.


Charles Clayton Dennie 1883-1971.

Charles Clayton Dennie 1883-1971.


Malignant lymphoma, reticulum cell type. Ultrastructural and cytological demonstration of Lutzner cells.


Contributions of renal biopsy studies to the understanding of disease.


A new approach to radical retroperitoneal iliac and femoral node dissection.


The injured hand. A panel by correspondence.


Preoperative oral antibiotic treatment. Healing of colonic anastomoses in the cat.


The effect of auxiliary liver transplantation on posthepatectomy coma in the dog.


Studies on the survival of Salmonella typhimurium and Salmonella bovismorbificans on pasture and in water.


Alkaline phosphatase and failure of calcification under the influence of a diphosphonate.


Hypervitaminosis A: a review.

Hypervitaminosis A: a review.


Polyamine metabolism in mouse liver after partial hepatectomy.


Destabilization of casein micelles by lysozyme.


Inhibition of fatty acid synthetase in Halobacterium cutirubrum and Escherichia coli by high salt concentrations.


The further purification of motilin, a gastric motor activity stimulating polypeptide from the mucosa of the small intestine of hogs.


Malignant lymphomas arising in Peyer's patches and other organs of untreated NZO-Bl mice.


Immunofluorescent studies of lymph nodes and spleens of leukotic cattle for cells producing IgM and IgG.


A reinvestigation of epidermal transplantation during chemical carcinogenesis.


Breakage of bacterial DNA by nitrofuran derivatives.


Tables for the identification of carotenoid pigments.


Functions and follies of the psychiatrist.


The Banting Memorial Lecture 1971. Physiology of insulin in man.


External silicone sponge with cryoplexy in the treatment of selected cases of retinal detachments.


Notes on some recent antibiotic literature. II. The new antibiotics.


Do cells fuse in vivo?

Do cells fuse in vivo?


Use of 131-I-fibrinogen in detection of renal transplant rejection.


Structural and functional changes leading to impaired bacterial elimination after overdistension of the rabbit bladder.


Renal transplant: a new technique in ureteral anastomosis experimental autotransplantation.


Sir Charles Bell (1774-1842).

Sir Charles Bell (1774-1842).


Synchrony in cultured animal cells.

Synchrony in cultured animal cells.


Cell contact phenomena.

Cell contact phenomena.


Antibody against neoplastic plasma cells. II. Suppressive effect on antibody-producing cells.


Immune response in vitro to Salmonella H-antigens, not affected by anti-theta serum.


Cellular formation of viriolytic antibody by mouse immunocytes: cytokinetics and specificity..


Potentiation of cultured mouse thymocyte responses by factors released by peripheral leucocytes.


Reticulosarcoma and amyloid development in BALB/c mice inoculated with syngeneic cells from young and old donors.


Studies on the susceptibility of C57BL/6 mice to Rauscher virus. II. Multiplication of Rauscher virus in C57BL/6 cells in vivo and in vitro.


Short-term effects of actinomycin D and nitrogen mustard on murine lymphocytic leukemia P388.


Physical retention of complete dentures.


Centric relation and condylar movement: anatomic mechanism.


Cytologic screening automated by Cytoscreener.


Stabilizing functional chew-in wax records.


Effect of venting on cast gold full crowns.


A rapid method for accurate cast duplication.


American board of prosthodontics.

American board of prosthodontics.


Natural relationships of indicator and pathogenic bacteria in stream waters.


A royal pharmacy in Melbourne.

A royal pharmacy in Melbourne.


Human renal allotransplants. Correlation of acute and chronic histologic alterations with endocrine function--a new syndrome?


Background of clinical cardiac electrostimulation. II. Electrotreatment of cardiac diseases with dysrhythmias incidental or absent.


Plastic surgery in the Army. A short historical account.


An evaluation of adults with repaired bilateral cleft lips and palates.


Evaluation of a non-narcotic analgesic, pentazocine, in burned children.


Stimulation of neovascularization--comparative efficacy of fresh and preserved skin grafts.


George H. Monks, M.D.: a neglected innovator.


Prolonged survival of kidney allografts after perfusion with RNA or DNA.


Perspectives of immunosuppression.

Perspectives of immunosuppression.


Immunological enhancement.

Immunological enhancement.


Hepaticojejunostomy for biliary obstruction.


Low dose radiation cancers in man.

Low dose radiation cancers in man.


William P. Longmire, Jr., M.D.

William P. Longmire, Jr., M.D.


Inhibition of pentagastrin-stimulated gastric secretion by duodenal acidification or administration of fat in normal subjects and in patients with duodenal ulcer.


Complement and tumor immunology.

Complement and tumor immunology.


Prevention and management of pancreatic fistula.


Field trials of methisazone as a prophylactic agent against smallpox.


Viral shedding in Chinese children following vaccination with HPV-77 and Cendehill-51 live attenuated rubella vaccines.


Concentrating function of the gallbladder.


Evaluation of Orenzyme in the prevention of swelling after hand surgery.


Medical aspects of diseases of the gallbladder and biliary tree.


Metabolism and transport of bile salts in the intestine.


Surgical aspects of biliary tract disease.


The regulation of the limb circulation in lymph-oedema (development of an arteriovenous shunt circulation in experimental lymph-oedema of the leg).


On the role of cyclic AMP in insulin release: II. Dynamic aspects and relations to adrenergic receptors in the perfused pancreas of adult rats.


Activation of sexual and aggressive behavior by androgen implanted into the male ring dove brain.


Tests of reciprocality in crossingover in partially diploid F  strains of Escherichia coli.


Scurvy as an occupational disease. V. Scurvy and the merchant marines.


Hydrocarbon cooxidation in microbial systems.


New indicator of histocompatibility differences in vitro.


Antigen receptors on the surface of antibody-secreting cells.


A study of cerebral atrophy in phenylketonuria. Sonoencephalographic examination of 45 PKU patients.


A specific antiserum for gastric mucous epithelium.


Influence of substrate on adult tracheal epithelium in culture.


Effect of temperature on commencement of migration of adult tracheal epithelial cells in culture.


The effect of symmetrel (amantadine hydrochloride) on a case of athetosis.


A chapter of early medical Africana.

A chapter of early medical Africana.


Treatment of severe parkinsonism with L-dopa.


Dr E. W. Turton.

Dr E. W. Turton.


Dr J. K. Bremer.

Dr J. K. Bremer.


University of Natal: honorary doctorate in sciences.


Effect of thromboendarterectomy upon muscle blood flow as measured by the clearance of  133 xenon.


The effect of traumatic and nontraumatic shock on allograft survival.


Splanchnic circulatory responses to ouabain in shock.


The comparative effectiveness of corticosteroids applied topically as pretreatment of rabbit skin allografts.


The history of lumbar sympathectomy.

The history of lumbar sympathectomy.


The natriuretic effect addition of quinethazone and furosemide in congestive heart failure. A permutation trial test.


A controlled study of peroral glucose, insulin and potassium treatment in myocardial infarction.


The supra-additive natriuretic effect addition of quinethazone or bendroflumethiazide during long-term treatment with furosemide and spironolactone. Permutation trial tests in patients with congestive heart failure.


Reversible and irreversible cytotoxic effects of isothiocyanates on HeLa-cells.


Light microscopic demonstration of acino-insular transformation.


Metabolic aspects of the climacterium.

Metabolic aspects of the climacterium.


Oestriol succinate in the treatment of climacteric women (a double-blind trial).


Complications following external cardiac massage, with special emphasis on cerebral embolism. Review of literature together with a case report.


Recent results of tRNA research.

Recent results of tRNA research.


Insect juvenile hormone analogues.

Insect juvenile hormone analogues.


Some personal history and reflections from the life of a biochemist.


Histones: structure and function.

Histones: structure and function.


Protein biosynthesis.

Protein biosynthesis.


Nutritional methodology in metabolic research with rats.


Clinical biochemistry.

Clinical biochemistry.


Evidence of phage induction in Streptococcus pyogenes by photodynamic action of 3,4-benzpyrene.


Sphingolipids.

Sphingolipids.


Lysogeny: viral repression and site-specific recombination.


Counseling. Basic guidelines for problem elimination.


Variable impression technic for crown 7 bridge and restorations.


Direct bonding of acrylic resins to tooth enamel.


Keynote address. We are delivering to about 20 percent of the population.


Physiologic and psychologic considerations in oral rehabilitation. II. Treatment of functional breakdown of natural dentition--the problem of success and failure.


The use of an epoxy type adhesive in crown and bridge repairs.


Stress reduction in removable partial dentures.


Pulmonary function compatibility of the time sharing computer system.


Infectious disease and community health.


Immunological studies with human neoplasms.


Reuben Leon Kahn, D.Sc., LL.D., M.D., Ph.D., 1887-.


Antitissue versus antimicrobial cellular immunity: a perspective.


The coolant controversy in operative dentistry.


Mechanisms in antimicrobial immunity.

Mechanisms in antimicrobial immunity.


Household agents and their potential toxicity.


Treatment of salicylate poisoning.

Treatment of salicylate poisoning.


Treatment of petroleum distillate ingestion.


Treatment of mercury poisoning.

Treatment of mercury poisoning.


Current treatment of potential tetanus: an ongoing problem of universal importance.


The current status of digitalis therapy.


Kidney transplantation in Massachusetts.


A practical approach to diagnosis of disorders of intestinal absorption.


R. Cannon Eley.

R. Cannon Eley.


Relationship between survival and thymine-dimer excision in ultraviolet-irradiated Escherichia coli.


Genetic damage induced by 254 nm ultraviolet light in Escherichia coli: 8-methoxypsoralen as protective agent and repair inhibitor.


Photoreversibility of U.V. lesions causing fermentation mutations in Escherichia coli.


The effect of caffeine on mutation frequency decline.


The epidemiology and genetics of rheumatoid arthritis.


Familial aggregation and twin studies in Still's disease. Juvenile chronic polyarthritis.


Gerald Hochschild M.R.C.S. (ENG.), L.R.C.P., (Lond.), D.A. (R.C.P. & S.) Eng. F.F.A.(R.C.S.) Eng.


Emile Schwartz, M.B., Ch.B.

Emile Schwartz, M.B., Ch.B.


Loma Linda Alumni 1972 dental directory.


Walter James Latham, M.R.C.S. (Eng.), L.R.C.P. (Lond.), D.M.R.D. R.C.P. (Lond), R.C.S. (Eng), F.F.R.


Disparate ventricular function in surgical patients.


Dog mixed lymphocyte culture during renal allograft rejection.


Pulmonary arterial blood flow to transplanted lungs of standing dogs.


The dog: an unsuitable animal for lung transplantation.


Efficacy of antilymphocyte globulin in cadaver renal transplantation.


Elimination of bronchial complications in lung transplantation.


A comparison of hypothermic preservation with hypothermic pulsatile perfusion in paired human kidneys.


The correlation of juxtaglomerular index with renin and erythropoietin levels in dog renal allografts.


Prolongation of dog renal allograft survival by allo-antisera to kidney and xenogeneic antilymphoid globulin.


Does splenectomy decrease the incidence of hyperacute rejection?


Anti-vascular endothelium antibody in renal transplantation.


Specificity of the heteroantibody in xenograft rejection.


The effect of modification of coagulation factors on xenograft rejection.


Prolonged survival of dog second set renal homografts using a heterologous anti-antiserum.


Determination of left ventricular ejection time in dog cardiac allografts.


Survival of rat heart allografts irradiated in vitro.


Immediate in vitro leucocyte DNA synthesis: an early indicator of heart allograft rejection.


Studies on cardiac "sweat".

Studies on cardiac "sweat".


The liver as a lymphocyte antigen modifier.


Antigenic and immunogenic capacities of cell membranes with and without antilymphocyte serum.


Leukocyte migration: a cellular index of renal allograft function.


Cyclophosphamide-induced immunologic tolerance to skin homografts.


Acid and pepsin secretion following parietal cell vagotomy.


Gastrin levels in anephric patients.

Gastrin levels in anephric patients.


Hemodynamic changes in clinical orthotopic liver transplantation.


Temporary auxiliary life-sustaining liver transplantation following acute hepatic necrosis.


The inter-liver competition and portal blood in regeneration of auxiliary liver transplants.


Hepatic competition in auxiliary allotransplantation.


Influence of the source of portal vein blood on dog auxiliary hepatic allograft function in recipients with hepatic insufficiency.


Tissue typing in penetrating keratoplasty.


Platelet activity in allograft rejection.


Growth potential of grafts, flaps, and tubes.


Augmentation of survival in pedicle skin flaps by the chemical production of ischemia.


Inhibiting collagen crosslinking on survival of skin allografts.


Isotopic measurement of collagen turnover in skin grafts.


Deep dermal burns treated by excision, skin grafts, and tri-iodothyronine.


Evaluation of formalin-fixed skin as a temporary dressing for granulating wounds.


Combined use of BCG and neuraminidase in experimental tumor immunotherapy.


Relief of diabetes mellitus by heterotopic transplantation of the whole pancreas in inbred rats.


Helen Whaley, M.D. 1924-1971.

Helen Whaley, M.D. 1924-1971.


A chest surgeon comes to Alaska.

A chest surgeon comes to Alaska.


Emulsion therapy: a three-year study with ragweed pollinosis.


Dynamic rigidity of F-actin-heavy meromyosin solutions.


Biological energy sources: a review.

Biological energy sources: a review.


Urinary polyamines in cancer patients.

Urinary polyamines in cancer patients.


The variation in twenty-one serum proteins before and after renal transplantation. I. General pattern.


The variation in twenty-one serum proteins before and after renal transplantation. II. Changes during acute rejection.


Cartesian diver studies on respiration and glycolysis of high and low sarcoma lines.


Normal and cancerous tissue transplantation in allogenic and syngeneic Xenopus laevis.


Criteria for involvement of lymph node, bone marrow, spleen, and liver in Hodgkin's disease.


Chemotherapeutic implications of staging in Hodgkin's disease.


Combined radiotherapy and chemotherapy of lymphomas and other cancers.


The disposition of doxycyline by man and dog.


Streptomycin resistance in some wild-type strains of Enterobacteriaceae.


Synergistic effect of combinations of sodium salicylate and antibiotics on strains of E. coli and Staphylococcus aureus.


Methods for purification of mixed cultures of Mycoplasma.


Fetoplacental effects of corynebacterium pyogenes in sheep.


The European Society for clinical investigation: a report of progress.


Calcification of the rat testis after local administration of formaldehyde.


Immunogenicity and specificity of collagen. XII. Demonstration by immunofluorescence and haemagglutination of antibodies with different specificity to human collagen.


Purification of chicken anti-bovine serum albumin antibody.


Complement-fixing and immunofluorescent antibodies to Mycoplasma pneumoniae in sera from a normal community.


Chronic cholecystitis and cholelithiasis: an experimental study.


Solitary benign enchondroma of the long bones of the hand.


Syndromes, antecedents, and outcomes of psychosis: a cluster-analytic study.


The advancement pedicle flap for thumb injuries.


Follow-up notes on articles previously published in the journal. Muscle-pedicle bone graft in hip fusion.


Personality patterns associated with heavy cigarette smoking in male college students.


A histor of the beginning of medicine in Indiana.


Roster of members, 1972.

Roster of members, 1972.


Human foetal pancreatic insulin secretion in response to ionic and other stimuli.


Quantitative reconstruction and superresolution of red-blood-cell image holograms.


Seroepidemiology of toxoplasmosis.

Seroepidemiology of toxoplasmosis.


Cooperation in research and communication toward the solution of global problems in parasitology.


Parasitology in the world today.

Parasitology in the world today.


The relationship between self-esteem and perceived reciprocal liking: a sociometric test of the theory of cognitive balance.


Behavioral sciences and cancer. A review.


Past-president's address. To assist young men and women in the study of physiology: the Porter development program.


Adrenocortical control of epinephrine synthesis.


Wallace O. Fenn. 1893-1971.

Wallace O. Fenn. 1893-1971.


Perspectives.

Perspectives.


Pathology of coronary atherosclerosis.

Pathology of coronary atherosclerosis.


Studies in myasthenia gravis: biologic aspects.


Pathophysiology of coronary circulation.


Pathophysiology of ischemic heart disease.


Specific hungers and poison avoidance as adaptive specializations of learning.


Anchorage of free-end dentures.

Anchorage of free-end dentures.


Contributions of the Mount Sinai Hospital to the study and care of patients with myasthenia gravis.


Procedure using transfer copings for inclined teeth.


Studies in myasthenia gravis: review of a twenty-year experience in over 1200 patients.


The "eccentric" repair system of Rotherman. II. A new method for the repairing of covered anchorages of partial and hybrid dentures.


Michael Somogyi, PH.D. (1883-1971).

Michael Somogyi, PH.D. (1883-1971).


Yusuf Dawood Docrat L.R.C.P. (Irel.) L.R.C.S. (Irel.).


Effect of physostigmine on the inhibitory action of scopolamine in man.


The mechanical work of breathing during controlled ventilation in normal subjects and patients with bronchial asthma.


A comparison between the thermal dilution method and the direct Fick and the dye dilution methods for cardiac output measurements in man.


A retrospective view of sensory analysis and some considerations for the future.


Gilles de la Tourette and his syndrome by serendipity.


Experimental production of Mycoplasma hyosynoviae arthritis in swine.


Role of colicins in antagonism between strains of Escherichia coli in dual-infected gnotobiotic pigs.


Endotoxin-induced mastitis in the guinea pig.


Improvement of myasthenia gravis by hemodialysis in a patient with chronic renal failure.


Insulin: fifty years ago.

Insulin: fifty years ago.


Immunofluorescent studies on the localization of viral structural components and surface antigen in FL cells infected with Chikungunya virus.


Rhythms, reproduction, and photoreception.


Sensory reception of olfactory cues.

Sensory reception of olfactory cues.


Studies on the ultra-structure of kinetoplasts of Trypanosoma cruzi and Trypanosoma gambiense by autoradiography and enzymatic digestion.


Rodent pheromones.

Rodent pheromones.


Veterans Administration Prosthetics Center research report.


Crown and inlay impression technic.

Crown and inlay impression technic.


High voltage electrophoresis.

High voltage electrophoresis.


Recording the vertical dimension of occlusion using the occlusion triangle.


An electrochemical method for the determination of blood supply of renal cortex before transplantation.


National health insurance and family planning.


Ammonia and urea in oral pathophysiology--a literature review.


Effect of urease inhibition on calculus formation and caries development in the rat.


Membranous glomerulonephritis: current status.


Study of an outbreak of cholera in Ahmedabad (1969).


Iowa Dental Association 1972 Membership Directory Active and Life members.


Wallace O. Fenn. 1893-1971.

Wallace O. Fenn. 1893-1971.


Prosthetic restorations from the aspect of gerodontology.


Unfolding of Escherichia coli ribosomes by phosphate ion in the presence of oligonucleotides.


Stereospecificity of the Escherichia coli valyl-RNA synthetase in the ATP- 32 PPi exchange reaction.


Coding properties os ultraviolet photoproducts of uracil. II. Phenotypic reversion of the amber mutation: implication of the uracil hydrate.


Physiologically induced changes in the property of phenylalanine tRNA in Escherichia coli.


Properties of sex factor and related episomes isolated from purified Escherichia coli zygote cells.


Diagnosis and treatment of familial lipoproteinemias.


RNA chain growth-rate in Escherichia coli.


Replication of bacteriophage DNA. I. Replication of DNA of lambda phage defective in early functions.


Judgment of spatial orientation in patients with focal brain damage. Thirty control and 121 brain-damaged patients with injury restricted to one hemisphere were presented with a test requiring the placing of a rod, fixed on a support by a hinged joint, in the same position as a model. Two versions of the test were given, one to be performed with the aid of vision and the other only by touch. The brain-damaged patients were subdivided into the following groups: right hemisphere patients without visual field defect (VFD): 30; right hemisphere patients with VFD: 20; left hemisphere patients without VFD: 46; left hemisphere patients with VFD: 25. The test modality did not differentially affect the performance of the groups. Both on the visually and on the tactually guided version of the test the right hemisphere group with VFD did significantly worse not only with respect to controls but also with respect to the three other braindamaged groups. The latter, in turn, were not significantly inferior to patients without cerebral damage. It appears, therefore, that when spatial perception is tested at a very basic and simple level, such as the detection of orientation in space of a rod, there is an almost complete dominance of the posterior region of the so-called minor hemisphere. This result must be contrasted with the less striking asymmetry of functions shown in more complex spatial tasks-for example, route finding, copying drawings, block designs-that are failed also by patients with damage to the left posterior area. Normal subjects reproduced the orientation of the rod on the horizontal plane with a constant error which was found to be dependent on the position of the model. Clockwise deviations were made when the model was on the left and counterclockwise deviations when the model was on the right. The constant error was present in left brain-damaged patients too, while it was not significantly different from zero in right brain-damaged patients. A systematic error was also found on the vertical plane and it consisted in the tendency to stop the rod below the level of the standard.


Three syndromes produced by two mutant genes in the mouse. Clinical, pathological, and ultrastructural bases of tottering, leaner, and heterozygous mice.


Disturbances of the body schema in relation to language impairment and hemispheric locus of lesion. The relationship of disturbances of the body schema to hemispheric locus of lesion and sensory aphasic disorder was assessed by giving verbal and non-verbal tests of right-left orientation, finger recognition, and autotopagnosis to patients with unilateral cerebral disease. The study was restricted to right-handed patients who were free from general mental impairment or confusion. The tests were also given to a group of control patients whose performances defined the range of normal performance in each test. A significant proportion of patients with sensory aphasic disorder performed defectively on all the tests, non-verbal as well as verbal, the relative frequency of failure in this group ranging from 10 to 67% for the different tests. There were, however, a number of patients with sensory aphasic disorder who performed adequately on most of the tests. Defective performance on the part of non-aphasic patients with lesions of either the left or the right hemisphere was quite rare in the case of 19 of the 20 tests. The exceptional test was the task of imitating lateral movements from Head's battery, on which both non-aphasic groups performed relatively poorly. The patients with lesions of the right hemisphere were significantly inferior to those with left hemisphere disease on this test. The findings are interpreted as indicating that sensory aphasic disorder is a necessary but not sufficient condition for the occurrence of some types of bilateral `body schema' disturbance in patients with unilateral disease. It is postulated that the sufficient condition is a combination of aphasic disorder with somatosensory impairment. Bilateral impairment of the `body schema' does not appear to have a differential relationship to hemispheric locus of lesion per se.


Serum creatine kinase studies in the detection of carriers of Duchenne dystrophy. A number of methods which might improve the detection of carriers of Duchenne muscular dystrophy, based on the estimation of serum creatine kinase (CK), have been tried in an attempt to improve the 70% detection rate obtained with random samples. One series of experiments involved controlled exercise on a bicycle ergometer, the second series was based on sampling of muscle venous blood after controlled ischaemic exercise, and a third series of experiments was carried out to see whether the rate of enzyme inactivation was different in carriers of Duchenne dystrophy compared with controls. These methods were not found to improve the carrier detection rate based upon serum CK estimation.


Stereological studies on vascularization phenomena. 4. Vascular system in the tubed pedicle flap on the gluteal region of the rabbit.


Colony formation and immunological studies on Treponema pallidum Kazan strain and Reiter strain.


Stereological studies on vascularization phenomena. 2. Vascular system in the pedicled flap in the rabbit's ear.


Psychosomatic aspects of Parkinsonism.

Psychosomatic aspects of Parkinsonism.


The clinical diagnosis of renal disease in the dog.


Essential features of the treatment of chronic renal failure in the dog.


The treatment and subsequent prevention of struvite urolithiasis in cats.


Comparative aspects of renal transplantation in man and dog.


Treatment of gout.

Treatment of gout.


The effects of vacuum on the tympanic cavity.


The use of urea in exudative otitis media.


Modification of surgical procedure for the exteriorization of the ureteral openings of the hen.


The Medical Club, or The Club of Nineteen. One hundred years of continuous existence. 1867-1967.


"Reminiscences of Olden Times".

"Reminiscences of Olden Times".


Memoir of Benjamin Greenspan. 1911-1970.


Memoir of Jacob Gershon-Cohen. 1899-1971.


Memoir of Frederick Louis Hartman. 1892-1970.


Memoir of Kelvin Anthony Kasper. 1899-1971.


Memoir of F. Maurice McPhedran. 1888-1970.


Memoir of Harold Hollingsworth Morris. 1884-1970.


Memoir of Charles Rupp. 1908-1970.

Memoir of Charles Rupp. 1908-1970.


Automated platelet counting.

Automated platelet counting.


Six-year clinical evaluation of the Swing-Lock removable partial denture.


The implant abutment and fixed prosthesis service.


Purification and properties of cytochromes c from trypanosomatids.


The management of polycythaemia vera.

The management of polycythaemia vera.


Cellular biology of anesthesia.

Cellular biology of anesthesia.


Advances in monitoring in the past 10 years.


Recent advances in uptake and excretion of inhalation anesthetics.


Premedication and drug interaction.

Premedication and drug interaction.


Advances in respiratory research related to anesthesia.


Recent advances in pediatric anesthesia.


Advances in local anesthesia and pain relief.


Advances in anesthesia for plastic surgery in burns.


Anesthesia for neurosurgical procedures.


Intensive care and inhalation therapy.

Intensive care and inhalation therapy.


Advances in electrosleep and electroanesthesia during the past decade.


Advances in cardiopulmonary resuscitation.


Anesthesia and the brain.

Anesthesia and the brain.


Carnitine acetyltransferase activity in Trypanosoma lewisi.


Potential hazards of ultrasonic amalgam condensation.


Glucose binding by intestinal brush borders of rats.


A clinical trial of alpha-methyldopa (Aldomet) and hydrochlorthiazide (Dichlotride) alone and in combination in hypertension.


Ninety-fifth critical bibliography of the history of science and its cultural influences (to January 1970).


Induction of colicin production by high temperature or inhibition of protein synthesis. Escherichia coli K-12 colicinogenic for ColE1 yielded mutants that appeared to produce colicin at 43 C but not at 30 or 37. These mutants proved to have the mutation recA(-) Further study revealed that both recA(-) and recA(+) bacteria, when carrying ColE1 or ColE2, produce more colicin during growth at higher temperatures or after brief exposure to temperatures beyond the growth range. Counts of lacunae demonstrated that the increase of colicin production is due to an increase in the number of cells that yield colicin. Heat treatment causes lacunae to increase by the same factor in recA(+) and recA(-) cells, although recA(-) bacteria produce 500 times fewer lacunae than recA(+). Inhibition of protein synthesis, notably by chloramphenicol, also induces colicin production in as much as 90% of the cells after removal of inhibition (to permit colicin synthesis). Induction of colicin production by chloramphenicol requires that ribonucleic acid synthesis continue during the period of inhibition. These results are discussed in relation to the regulation of colicin production.


Metabolism of 4-N-hydroxy-cytidine in Escherichia coli. 4-N-hydroxy-cytidine was found to substitute for uridine as a pyrimidine supplement for the growth of Escherichia coli Bu(-). Measurement of the incorporation of 4-N-hydroxy-cytidine-2-(14)C into ribonucleic acid and deoxyribonucleic acid revealed that this compound was converted to cytidine or uridine before utilization. Two pathways for metabolism were considered: (i) the reduction of 4-N-hydroxy-cytidine to cytidine followed by deamination, (ii) the direct hydrolysis of hydroxylamine from 4-N-hydroxy-cytidine to yield uridine. A threefold increase in cytidine (deoxycytidine) deaminase (EC 3.5.4.5) activity, when the cells were grown on 4-N-hydroxy-cytidine, suggested the involvement of this enzyme. More direct proof was obtained by purifying the deaminase 185-fold and finding that it released hydroxylamine from 4-N-hydroxy-cytidine at one-fiftieth the rate at which ammonia was removed from cytidine. This result is consistent with the slower rate of growth of the Bu(-) cells on 4-N-hydroxy-cytidine than cytidine and suggests that the second pathway is the major route for utilization of this compound.


Lethal synthesis of methylglyoxal by Escherichia coli during unregulated glycerol metabolism. In Escherichia coli K-12, the conversion of glycerol to triose phosphate is regulated by two types of control mechanism: the rate of synthesis of glycerol kinase and the feedback inhibition of its activity by fructose-1,6-diphosphate. A strain which has lost both control mechanisms by successive mutations, resulting in the constitutive synthesis of a glycerol kinase no longer sensitive to feedback inhibition, can produce a bactericidal factor from glycerol. This toxic factor has been identified by chemical and enzymological tests as methylglyoxal. Methylglyoxal can be derived from dihydroxyacetone phosphate through the action of an enzyme which is present at high constitutive levels in the extracts of the mutant as well as that of the wild-type strain. Nine spontaneous mutants resistant to 1 mm exogenous methylglyoxal have been isolated. In all cases the resistance is associated with increased levels of a glutathione-dependent enzymatic activity for the removal of methylglyoxal. Methylglyoxal-resistant mutants derived from the glycerol-sensitive parental strain also became immune to glycerol.


Succinate as a growth factor for Bacteroides melaninogenicus. Rumen strains of the obligate anaerobe Bacteroides melaninogenicus normally require medium supplemented with both heme and vitamin K. Sodium succinate was found to be an additional growth factor in that this compound can replace the requirement for heme in the presence of vitamin K, allowing good growth of the organism, and succinate can also partially replace the requirement for vitamin K in the presence of heme. The addition of succinate to a medium supplemented with both vitamin K and heme increases the growth rate of the culture. This ability to stimulate growth was specific for succinate, and cells grown without heme but with vitamin K and succinate were insensitive to cyanide. These experiments demonstrate a central role for succinate in the metabolism of B. melaninogenicus.


Deoxyribonucleic acid-ribonucleic acid hybridization studies on the L-Arabinose operon of Escherichia coli B-r. An increase in the rate of synthesis of ara-specific messenger ribonucleic acid as measured by deoxyribonucleic acid-ribonucleic acid hybridization has been detected in the induced wild-type (ara(+)) strain of Escherichia coli B/r as compared with the uninduced control, thus providing evidence that regulation of the positively controlled l-arabinose operon is at the level of transcription.


Gas-vacuolated strains of Microcyclus aquaticus. Two strains of gas-vacuolated heterotrophic bacteria have been isolated from freshwaters by using 0.01% peptone enrichments. Both are nonmotile, vibrioid organisms that form rings before division. In terms of these morphological traits and other characteristics, including nutritional characters and deoxyribonucleic acid base composition, these new isolates are very similar to Microcyclus aquaticus. It is proposed that they be considered strains of this species.


Pseudomonas putida tryptophan synthetase: partial sequence of the   subunit. There is 50% identity in the sequences of the first 50 residues of the alpha chains of Escherichia coli and Pseudomonas putida. No deletions or additions of residues are found in this region, except for the N-terminal methionine residue which is missing in the polypeptide isolated from P. putida. Most of the residues which differ are chemically dissimilar, and half of them are specified by codons which differ by more than a single base. The two residues known by mutational analysis to be essential for catalysis in E. coli are conserved in P. putida. The potential taxonomic usefulness of information of this sort is analyzed.


Polysome turnover during amino acid starvation in Escherichia coli. The experiments presented in this paper support earlier evidence that ribosomes are released from polysomes when they encounter a codon for which no charged transfer ribonucleic acid is available. However, it is further shown that these ribosomes then reinitiate and resume translation. The size and the level of polysomes during deprival of an amino acid is a function of the frequency with which that particular amino acid appears in cellular proteins. Polysomes from starved cells are more stable than those from growing cells, and, moreover, polysomes from starved relaxed strains are more stable than those from starved stringent strains.


Regulation of synthesis of the aminoacyl-transfer ribonucleic acid synthetases for the branched-chain amino acids of Escherichia coli. The regulation of synthesis of valyl-, leucyl-, and isoleucyl-transfer ribonucleic acid (tRNA) synthetases was examined in strains of Escherichia coli and Salmonella typhimurium. When valine and isoleucine were limiting growth, the rate of formation of valyl-tRNA synthetase was derepressed about sixfold; addition of these amino acids caused repression of synthesis of this enzyme. The rate of synthesis of the isoleucyl- and leucyl-tRNA synthetases was derepressed only during growth restriction by the cognate amino acid. Restoration of the respective amino acid to these derepressed cultures caused repression of synthesis of the aminoacyl-tRNA synthetase, despite the resumption of the wild-type growth rate.


Influence of metal ions on the formation of mycobactin and salicylic acid in Mycobacterium smegmatis grown in static culture. Mycobacterium smegmatis was grown on trace-metal-free medium in static culture. Throughout the growth phase, the concentration of mycobactin increased continuously, reaching a maximum of about 30 to 40 mug of mycobactin/mg of cell dry weight after 6 days; the concentration of salicylic acid remained approximately constant at 1 to 2 mug of salicylic acid/mug of cell dry weight. Fe(2+) (or Fe(3+)), Zn(2+), Mn(2+), and Mg(2+) were all essential to a maximum formation of mycobactin. Optimum concentrations required were: Fe(2+), about 1.8 mum; Mn(2+) and Zn(2+), about 0.5 mum; and Mg(2+), at least 0.17 mm. Higher levels of Fe(2+) (9 to 90 mum) and Zn(2+) (2 to 7 mum) repressed mycobactin to about half the maximum value. No other cation or anion apparently is required for mycobactin biosynthesis. Salicylic acid concentration increased about fourfold when iron was omitted from the medium, but this is not as great as the increase reported previously for this strain of M. smegmatis. Mycobactin formation in another strain of M. smegmatis, NCIB 8548, showed similar dependencies on Fe(2+), Zn(2+), and Mn(2+). Maximum accumulation of mycobactin with this strain was 85 mug of mycobactin/mg of dry cell weight, under iron-deficient (1.8 mum Fe(2+)) conditions.


Physical and functional characteristics of R-factor deoxyribonucleic acid segregated into Escherichia coli minicells. Both fi(+) and fi(-) type R factors function as intact replicative units when segregated into Escherichia coli minicells. Hybridization studies demonstrate that at least 95% of the deoxyribonucleic acid (DNA) in R(+) minicells is episomal in origin. About half of the DNA can be extracted in a closed circular form and about 75% of the DNA is membrane associated. DNA, ribonucleic acid, and protein synthesis proceeds in R(+) minicells in contrast to R(-) minicell controls. The system offers a unique opportunity to study a relatively small replicative unit in a native cell environment and a simple means of isolating large quantities of episomal DNA.


Antibacterial nitroacridine, Nitroakridin 3582: effects on bacterial growth and macromolecular biosynthesis in vivo. The antibacterial drug Nitroakridin 3582 inhibited the growth of selected grampositive bacteria more strongly than it inhibited the growth of gram-negative bacilli. Nitroakridin at concentrations of the order of 5 x 10(-5)m induced lysis of Bacillus licheniformis and Micrococcus lysodeikticus. At concentrations less than 10(-4)m, Nitroakridin 3582 reduced the exponential growth rate of Escherichia coli C-2; at 10(-4)m the drug was bacteriostatic, and, at concentrations greater than 10(-4)m, it was bactericidal. Prolonged bacteriostasis resulted in the formation of long filaments by E. coli, Serratia marcescens, Shigella sonnei, and Proteus mirabilis. The reversible effects of Nitroakridin 3582 on the growth of E. coli correlated with partial inhibitions of deoxyribonucleic acid biosynthesis; ribonucleic acid and protein syntheses were inhibited less strongly. Nitroakridin 3582 at concentrations greater than 2 x 10(-4)m, which block deoxyribonucleic acid biosynthesis, produced an accelerated bactericidal action.


Control of vitamin B 6  biosynthesis in Escherichia coli. Pyridoxineless mutants of Escherichia coli B which specifically require pyridoxal or pyridoxamine for growth can be divided into classes according to their growth responses in enriched media. Members of the slowest growing class synthesize vitamin B(6) at the fastest rates when starved for pyridoxal in glycerol minimal medium. After 80 min of synthesis at 4 x 10(-10) moles of vitamin B(6) per mg of cells per hr, the rate increases four- to fivefold and continues at the new rate for several hours. The shift to the new rate is prevented by chloramphenicol, thus suggesting that a derepression mechanism exists to control vitamin B(6) synthesis in addition to the previously discovered feedback control.


Salmonella typhimurium mutants with alternate requirements for vitamin B 6  or isoleucine. Several mutants of Salmonella typhimurium LT-2, isolated as auxotrophs for vitamin B(6), grew without the added vitamin when supplied with either isoleucine, alpha-ketobutyrate, or alpha-keto-beta-methylvalerate, but not with threonine or with other alpha-keto acids. When grown on minimal medium supplemented with isoleucine, these mutants synthesized vitamin B(6) in amounts comparable to wild-type cells; they thus appeared to contain a modified l-threonine dehydratase and to belong to genotype ilvA (threonine dehydratase) instead of pdx (pyridoxine). Direct assays confirmed this hypothesis. Wild-type cells (toluene-treated) showed approximately the same threonine dehydratase activity whether grown in the presence or absence of added pyridoxal-P; mutant cells approached the activity of wild-type cells only when they were grown with added vitamin B(6) and were assayed in the presence of pyridoxal-P. In cell-free extracts, the threonine dehydratase from mutant cells was cold labile and more labile to oxidative inactivation than the wild-type enzyme; furthermore, activation of the mutant apoenzyme required a 10- to 20-fold higher concentration of pyridoxal-P than was required for the wild-type apoenzyme. These results show that cultures which appear auxotrophic for a given vitamin may synthesize that vitamin in normal amounts, the exogenous requirement arising from impaired binding of the vitamin-derived coenzyme to a genetically altered apoenzyme dependent on that coenzyme. Inadequate nutritional data to support the genetic findings can lead to erroneous genotype classification for such mutants.


Pseudomonas putida tryptophan synthetase. The two protein components of Pseudomonas putida tryptophan synthetase have been purified to homogeneity. Although there is general similarity between the Pseudomonas enzyme and that of the enteric bacteria, many differences were found. Components from Escherichia coli and P. putida do not stimulate each other enzymatically, and the enzymes differ in their response to monovalent cations. Serine deamination occurs best with the intact enzyme of P. putida, not with the beta(2) subunit alone as in E. coli. The amino acid compositions of the alpha subunits differ appreciably. These findings extend earlier studies showing differences between enteric organisms and pseudomonads in the regulation and genetic organization of the enzymes of the tryptophan pathway.


Nature of the penetration barrier in Escherichia coli K-12: effect of macromolecular inhibition of penetrability in strains containing the envA gene. The envA mutation in Escherichia coli K-12, which maps at 1.5 min, was previously shown to mediate sensitivity to gentian violet as well as to several antibiotics. Moreover, strains containing the envA gene were recently found to be lysed by lysozyme in the absence of ethylenediaminetetraacetate. It is here reported that the envA mutation mediates an increased uptake of gentian violet. The uptake of the dye was markedly affected by growth with different antibiotics interfering with macromolecular synthesis. Amino acid starvation of a strain containing envA with a stringent control of ribonucleic acid (RNA) synthesis resulted in a decreased uptake of gentian violet. However, no decrease in dye uptake was found during starvation in an envA transductant with a relaxed control of RNA synthesis. Inhibition of deoxyribonucleic acid (DNA) synthesis by nalidixic acid decreased the uptake of gentian violet of envA cells and, in addition, rendered the cells insensitive to the lytic action of lysozyme. Chloramphenicol treatment increased penetrability in wild-type and starved envA cells. In most instances, this effect of chloramphenicol was prevented by selectively interfering with DNA or RNA synthesis. A coordinate regulation of nucleic acid synthesis and penetrability is suggested.


Fracture faces in the cell envelope of Escherichia coli. Freeze-fracturing of Escherichia coli cells in the presence of 30% (v/v) glycerol resulted in a double cleavage of the cell envelope exposing two convex and two concave fracture faces ([Formula: see text], [Formula: see text] and [Formula: see text], [Formula: see text]) with characteristic patterns. Complementary replicas revealed the relationship of the fracture faces to their corresponding fracture planes. The inner fracture plane splits the plasma membrane at one particular level. Apparently the outer fracture plane was located in the outer part of the wall, as it was separated by a layer ([Formula: see text]) from the fractured profile (CW1) presumably corresponding to the murein layer. The outer fracture plane did alternate toward the cell periphery, exposing complementary smooth areas ([Formula: see text] and [Formula: see text]). When cells were freeze-fractured in the absence of glycerol, the outer cell surface appeared as an etching face rather than a fracture face. A schematic representation of the relative location of the different fracture faces in the E. coli cell envelope is given.


Phenethyl alcohol as a suppressor of the envA phenotype associated with the envA gene in Escherichia coli K-12. In Escherichia coli K-12 the envA gene was previously shown to mediate chain formation and a decreased tolerance to several antibacterial agents. Phenethyl alcohol at low concentrations has now been found to increase the tolerance to actinomycin D, ampicillin, rifampin, and gentian violet in strains containing envA. The increased tolerance to gentian violet was correlated to a decreased uptake of the dye. A phenotype suppression of chain formation and colony morphology in envA mutants was also obtained. Except for an increase in palmitic acid, chemical analysis revealed no differences between an envA and its wild-type strain in the lipopolysaccharide part of the envelope. However, a decrease in the amount of phosphatidylglycerol and a C18: 1 fatty acid was observed in the extractable lipids of a strain containing envA. Growth in the presence of phenethyl alcohol reversed the changes in fatty acid and the phospholipid composition. Phenethyl alcohol was found to cause an immediate but transient inhibition of ribonucleic acid synthesis. It is suggested that this inhibition affects the penetrability barrier of the outer cell envelope layers in strains containing envA.


Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100. Treatment of a partially purified preparation of cell walls of Escherichia coli with Triton X-100 at 23 C resulted in a solubilization of 15 to 25% of the protein. Examination of the Triton-insoluble material by electron microscopy indicated that the characteristic morphology of the cell wall was not affected by the Triton extraction. Contaminating fragments of the cytoplasmic membrane were removed by Triton X-100, including the fragments of the cytoplasmic membrane which were normally observed attached to the cell wall. Treatment of a partially purified cytoplasmic membrane fraction with Triton X-100 resulted in the solubilization of 60 to 80% of the protein of this fraction. Comparison of the Triton-soluble and Triton-insoluble proteins from the cell wall and cytoplasmic membrane fractions by polyacrylamide gel electrophoresis after removal of the Triton by gel filtration in acidified dimethyl formamide indicated that the detergent specifically solubilized proteins of the cytoplasmic membrane. The proteins solubilized from the cell wall fraction were qualitatively identical to those solubilized from the cytoplasmic membrane fraction, but were present in different proportions, suggesting that the fragments of cytoplasmic membrane which are attached to the cell wall are different in composition from the remainder of the cytoplasmic membrane of the cell. Treatment of unfractionated envelope preparations with Triton X-100 resulted in the solubilization of 40% of the protein, and only proteins of the cytoplasmic membrane were solubilized. Extraction with Triton thus provides a rapid and specific means of separating the proteins of the cell wall and cytoplasmic membrane of E. coli.


Alterations in the cytoplasmic membrane proteins of various chlorate-resistant mutants of Escherichia coli. Chlorate-resistant mutants corresponding to each known genetic locus (chlA, chlB, chlC, chlD, chlE) were isolated from Escherichia coli K-12. All these mutants showed decreased amounts of membrane-bound nitrate reductase, cytochrome b, and formic dehydrogenase, but all had normal succinic dehydrogenase activity. Proteins from the cytoplasmic membranes of these mutants were compared to those of the wild type-on polyacrylamide gels. The addition of nitrate to wild-type anaerobic cultures caused increased formation of three membrane proteins. These same proteins, along with one other, were missing in varying patterns in mutants altered at the different genetic loci. One of the missing proteins was found to be the enzyme nitrate reductase, although this protein was present in some mutants lacking nitrate reductase activity. None of the others has been identified.


Genetic evidence for subunits in the fertility repressor produced by F-like R factors. Repressor-negative mutants derived from R 100 were tested for genetic complementation. The results indicate that the active repressor is a multimer. The properties of an unusual repressor mutant are also discussed.


Heat stabilities of ribosomal subunits and reassociated ribosomes from Bacillus stearothermophilus. Absorbance-temperature profiles reveal that both the 30S and 50S ribosomal subunits from Bacillus stearothermophilus are more thermostable than the comparable Escherichia coli particles. Thermophile ribosomes formed by the reassociation of subunits do not display functional heat stability.


Inhibition of ribonuclease II of Escherichia coli by sodium ions, adenosine-5'-triphosphate, and transfer ribonucleic acid. Ribonuclease II action on polyuridylate is competitively inhibited by transfer ribonucleic acid and noncompetitively inhibited by sodium ions. At low substrate levels, adenosine-5'-triphosphate is also inhibitory.


Transposition of the lac region to the gal region of the Escherichia coli chromosome: isolation of lambda-lac transducing bacteriophages. Defective and plaque-forming lambdalac transducing phages have been isolated from bacterial strains in which the lactose operon has been genetically transposed to the galactose locus.


Transcription of the operator proximal and distal ends of the tryptophan operon: evidence that trpE and trpA are the delimiting structural genes. Measurements of the time required for transcription of regions of known genetic length in the tryptophan operon of Escherichia coli indicate that there are no major unidentified structural genes in the operon either before the operator proximal E gene or after the operator distal A gene.


Nucleoside triphosphate pools in synchronous cultures of Escherichia coli. Endogenous nucleoside triphosphate pools in synchronized cultures of Escherichia coli B/r/1 oscillate as a function of age. Purine nucleoside triphosphates show a gradual 50% increase from zero age to the time of subsequent division, immediately prior to division. In contrast, pyrimidine nucleoside triphosphates undergo a dramatic change of about 50% in the first half of the generation at a time coincident with the termination of a round of deoxyribonucleic acid replication. A 50 to 70% increase starts at the initiation of the next round of deoxyribonucleic acid replication and continues until cell division, in parallel with the purine nucleotides. The fluctuation of pyrimidines between zero age and the middle of the division cycle suggests a functional relationship for pyrimidine metabolism and the regulation of cell division.


Stimulation of insulin release by a ventro-lateral hypothalamic factor.


The influence of the antecedent diet upon glucagon and insulin secretion.


Role of prosthodontist in the treatment of fractures of the jaws.


Pandemic dengue in Caribbean countries and the southern United States--past, present and potential problems.


The free-end saddle partial denture.

The free-end saddle partial denture.


Outbreak of hepatitis in a renal transplantation unit.


Sara A. Bonnett, M.D. 1897-1971.

Sara A. Bonnett, M.D. 1897-1971.


Seymour L. Lustman, M.D. 1920-1971.

Seymour L. Lustman, M.D. 1920-1971.


Annie Reich, M.D. 1902-1971.

Annie Reich, M.D. 1902-1971.


Edoardo Weiss, M.D. 1889-1970.

Edoardo Weiss, M.D. 1889-1970.


Elizabeth Rosenberg Zetzel, M.D. 1907-1970.


Gunshot wounds of the South African War.


Actinomycosis.

Actinomycosis.


The disulfide bonds of viscotoxin A3 from the European mistletoe (Viscum album L., Loranthaceae).


Regulation of tryptophanase formation in Escherichia coli.


Comparative structural studies on the M-antigen from Salmonella typhimurium. Escherichia coli and Aerobacter cloacae.


Traumatic diaphragmatic hernia.

Traumatic diaphragmatic hernia.


Myasthenia gravis: past and future.

Myasthenia gravis: past and future.


Ratios of protein fractions to DNA and electrophoretic studies of histones of diploid and polyploid nuclei of rat liver.


The structure of a phosphopeptide derived from  -casein.


Degradation of RNA in rat reticulocytes. II. Relationship between RNase and its corresponding inhibitor in rat reticulocyte.


Unusual growth properties of a bacterial strain lacking DNA polymerase.


On the nature of the polymerase responsible for the transcription of released bacterial DNA in plant cells.


Determination of an upper limit to the phosphorus content of polypeptide chain elongation factor and ribosomal proteins in Escherichia coli.


The dependence of raman scattering on the conformation of ribosomal RNA.


Isolation of cold sensitive-rifampicin resistant RNA polymerase mutants of Escherichia coli.


A history of jaundice in the newborn.

A history of jaundice in the newborn.


Quantitative assessment of the effects of light treatment in infant Gunn rats.


Photodegradation products of bilirubin studied in myelinating cerebellum cultures.


Effect on neonatal hyperbilirubinemia of phenobarbital during pregnancy or after birth: practical value of the treatment in a population with high risk of unexplained severe neonatal jaundice.


Lymphocyte activation. I. Response of T and B lymphocytes to phytomitogens.


Organ specificity of antithymocyte globulin.


Surface peptido-glycolipid filaments on Mycobacterium leprae.


Cholinesterase inhibition by organophosphorus compounds and its clinical effects. The clinical manifestations of acute poisoning by organophosphorus compounds in man are in accord with, initially, the stimulation and, later, the blocking of cholinergic transmission due to acetylcholinesterase inhibition. The manifestations involve mainly the para-sympathetic nerves, the neuromuscular junctions, and the central nerve synapses, and to a smaller degree the cholinergic sympathetic nerves. Miosis and muscle fasciculations are useful signs for diagnosis and for the control of therapy. Blood cholinesterase determination is the best diagnostic test. The cause of death is usually respiratory paralysis. Persistent manifestations have not been confirmed. Atropine and pralidoxime are effective for treatment and useful for diagnosis. Other oximes are promising but their clinical value has not been established. Poisoning by malathion is characterized by a prolonged course and by motor signs. Poisoning by organophosphorus compounds in man differs from animal experiments in several ways: in man, exposure may occur by several different routes, the manifestations are detected more easily, and therapy is given throughout the course of illness.


Schiffer S:Spasmodic torticollis; results after cervical rhizotomy in 80 cases.


Leg blood flow during exercise in man.

Leg blood flow during exercise in man.


Ethylene oxide sterilization--past and future.


The distribution of platelets, fibrin and erythrocytes in various organs following experimental trauma.


Validity of blood volume determinations in hemorrhagic shock in rats.


The pharmacological influence on thrombocyte aggregation in the extracorporeal circulation of dogs.


The value of skin allografts in the evaluation of the rejection of liver allotransplants in pigs.


Introduction to discussion of regulation of erythrocyte glycolysis.


Metabolism of red cell glutathione.

Metabolism of red cell glutathione.


Sulfhydryl groups in red cell membranes.


Introduction to discussion of red cell GSH metabolism: the functions of reduced glutathione in human erythrocytes.


Mechanisms of hemoglobin precipitation into Heinz bodies: possible relevance to cataract formation.


Introduction to discussion of Heinz body anemias--unstable hemoglobinopathies.


Galactose cataract: a review.

Galactose cataract: a review.


Glucose-6-phosphate dehydrogenase deficiency: mechanisms of drug-induced hemolysis.


Hypothalamic follicle-stimulating hormone (FSH) and luteinizing hormone (LH)-regulating hormone: structure, physiology, and clinical studies. A new concept of a single hypothalamic releasing hormone which is capable of releasing either LH or FSH (luteinizing hormone or follicle-stimulating hormone) is presented, and studies are described which summarize its physiological and  chemical properties.  Porcine LH-RH (LH releasing hormone) in a high state of purity was found to stimulate in rats the release of LH and FSH; this also occurred in humans.  The hormone was isolated and was found to consist of 10 amino acids; the hormone has been synthesized, and the synthetic material has been shown to possess the same properties as the natural material in rats: stimulation of the release of LH  and FSH in vitro and in vivo.  Addition of nanogram amounts of natural porcine LH-RH/FSH-RH or synthetic hormone is capable of stimulating both release and synthesis of LH and FSH in vitro.  12 steroids used for contraception did not block the release of LH and FSH after administration of LH-RH.  There is some evidence that progesterone pretreatment blocks the response to LH-RH, while estrogen seems to increase the response to LH-Rh in rats.  Clinical studies clearly demonstrated the effectiveness of porcine LH-RH in stimulating the release of LH in the human.  The smallest dose which induced a significant rise (p=.01) in LH was 10mcg.  LH levels were elevated in prepubertal children as well as in normal adults by the hormone.  One woman with secondary amenorrhea ovulated and conceived after treatment with LH-RH.  This and other clinical studies suggest LH-RH may be useful in the treatment of fertility and as a diagnostic aid in distinguishing between pituitary and hypothalamic disorders.


Endocrine tumour in kidney affecting small bowel structure, motility, and absorptive function. A 44-year-old woman is described with an endocrine tumour arising in the kidney. There were associated abnormalities of small intestinal morphology, motility, and absorptive function. These abnormalities reversed on removal of the tumour. Detailed studies showed that the tumour contained, and was secreting, glucagon. It is postulated that the intestinal abnormalities may have resulted from glucagon itself or another, as yet unidentified, hormone.


Intestinal lymphoma and sprue: a systematic approach. Intestinal lymphoma is frequently associated with sprue-like bowel patterns. A standardized approach to the problem is suggested.Intestinal lymphoma has been subdivided into four groups, depending on the extent of involvement of the anatomical region. Only cases with specific lesions are accepted as primary lymphoma. The villous pattern in 179 cases at necropsy with suitably preserved mucosa was determined to form a normal baseline for comparison with the uninvolved portions of the lymphomatous small bowel. Only 15% of all small bowel of this random material of children more than 1 year old and adults showed a flattened mucosa. The non-lymphomatous mucosa of 20 cases with definitely primary intestinal reticulum or lymphosarcoma showed severe sprue-like atrophy in 18 (90%). Two cases of intestinal Hodgkin's disease and four cases of gastric lymphoma were associated with regular mucosal patterns. It is concluded that sprue-like villous atrophy of the small bowel is definitely a triggering factor for the development of primary intestinal reticulum cell or lymphosarcoma. A hypothesis for the possible aetiological relationship of these two conditions is discussed.


Progress report. Anal continence.

Progress report. Anal continence.


Bacterial agglutination by secretory IgA from human gastrointestinal secretions and colostrum.


A double-blind trial of azathioprine in Crohn's disease.


The effect of vagotomy upon pepsin I secretion in man and its possible role in duodenal ulceration.


Farewell to Ludwig Heilmeyer (1899-1969).


Reconstruction after traumatic mutilations of the hand.


Effects of fluocinolone acetonide cream on the skin window record of inflammatory exudates.


Tissue culture of urinary bladder tumor induced in a rat by N-butyl-N-(-4-hydroxybutyl)nitrosamine: establishment of cell line, Nara Bladder Tumor II.


Splenectomy in renal transplantation. Influence on azathioprine sensitivity.


Effects of hypoglycemic agents on vascular complications in patients with adult-onset diabetes. 3. Clinical implications of UGDP results.


Mary E. Switzer 1900-1971.

Mary E. Switzer 1900-1971.


Serial determinations of homovanillinic acid in the cerebrospinal fluid of Parkinson patients treated with L-dopa.


BC 105 and methysergide (Deseril) in migraine prophylaxis.


Glomerular lesions in adults with the Schönlein-Henoch syndrome. A light and electron microscopy study.


Correlation between tubules and capillaries and size of interstitial space in the functioning rat kidney. Influence of different types of preparation.


Degree of malignancy of cancer involving the cervix uteri, judged on the basis of clinical stage, histology, size of nuclei, and content of DNA.


Metabolism of chloroacetate-1- 14 C in the mouse.


Quantitative determination of single-stranded sections in DNA using the fluorescent probe acridine orange.


A convenient solid-state substrate for assays of polynucleotide ligase: the poly(dA)-(poly(( 3 H)dT)-poly(dT))-cellulose.


Biological activity of  -phenylethanol and its derivates. V. Influence on DNA and RNA synthesis in different in vitro systems.


A general method for the separation of isoaccepting transfer ribonucleic acids: purification of five leucine transfer ribonucleic acids from Escherichia coli.


The "fragment reaction": a tool for the discrimination between cytoplasmic and mitochondial ribosomes.


Asparaginyl-transfer RNA: a substrate for L-asparaginase.


Maturation of 5-S RNA in Salmonella typhimurium.


Selective inactivation of amino acid acceptor and ribosome-binding activities of Escherichia coli tRNA by modification with cyanogen bromide.


Role of the ribosome in stringent control of bacterial RNA synthesis.


Restrictive effect of prophage lambda on DNA degradation in Escherichia coli cells.


Effect of ionic strength and temperature on the in vitro transcription of T 4  DNA.


Separation and properties of DNA polymerases from developing rat brain.


Primary tumours of bone--their classification and incidence.


Fluorescent treponemal antibody tests on cerebrospinal fluid.


Immunological studies on treponemal antigens. II. Serological changes and resistance to infection in rabbits immunized with culture supernatant of avirulent Treponema pallidum.


Formation of alkali-stable pepsin complexes from human gastric juice pepsin.


Harvey Society. Active members.

Harvey Society. Active members.


The streptococcal cell wall.

The streptococcal cell wall.


Pulmonary artery-left heart circulatory transfer function in hemorrhagic hypotension.


Controlled variation of light intensity to test frequency responses of densitometers.


Disulfiram treatment of alcoholism.

Disulfiram treatment of alcoholism.


Hepatic changes in Hodgkin's disease.

Hepatic changes in Hodgkin's disease.


Pregnancy as an experimental system for the study of immunological diseases.


Overlay or tooth supported dentures.

Overlay or tooth supported dentures.


Renal changes in loiasis.

Renal changes in loiasis.


Normal and mutant glycine transfer RNAs.


DNA synthesis in lysates of RecB- and Rec+ E. coli cells.


Medical and surgical aspects in the treatment of congenital hydrocephalus.


A dental epidemiological survey of a Northern Ireland population. Prosthetic findings.


Julius Friedlander, M.B., CH.B., D.P.H., M.D.


Nathaniel Fram, M.B., CH.B. (Edin.). D.M.R.E. (Cantab).


Influence of metyrapone on plasma concentrations of testosterone and androstenedione in man.


Surgery of the adrenal glands.

Surgery of the adrenal glands.


Biologic principles affecting repair of flexor tendons.


Diverticular disease of the colon.

Diverticular disease of the colon.


The present status of tumor immunology.

The present status of tumor immunology.


Intra-abdominal sepsis.

Intra-abdominal sepsis.


Recent advances in clinical diagnostic roentgenology. 2. Pharmacoradiology.


Recent advances in clinical diagnostic radiology. 4. Interventional diagnostic roentgenology.


Recent advances in clinical diagnostic radiology. 3. Contrast media.


The oral contraceptive controversy.

The oral contraceptive controversy.


Bernard Pierre Widmann 1890-1971.

Bernard Pierre Widmann 1890-1971.


Prevalence of extrachromosomal drug resistance. Bacterial drug resistance in animals.


A central component in the analgesic action of sodium salicylate.


Microorganisms as test systems for mutagenicity.


The amino acid sequences of erabutoxins, neurotoxic proteins of sea-snake (Laticauda semifasciata) venom. 1. Erabutoxin b was reduced, S-carboxymethylated and hydrolysed with trypsin. Seven tryptic fragments were isolated by column chromatography and paper electrophoresis. Some of the fragments were further hydrolysed with alpha-chymotrypsin, pepsin, Nagarse, Proctase A or Proctase B. The amino acid sequences of the fragment peptides were determined by subtractive Edman degradation. 2. From the tryptic digest of reduced, S-carboxymethylated and trifluoroacetylated erabutoxin b two fragments were isolated. From the amino acid composition of the fragments and from the terminal sequence studies on the reduced and S-carboxymethylated erabutoxin b, the sequence of the above seven tryptic fragments was elucidated. 3. The tryptic digestion of reduced and S-carboxymethylated erabutoxin a gave fragments, only one of which was different from the corresponding fragment from erabutoxin b. The amino acid sequence analysis of the fragment peptide showed that the only difference between erabutoxins a and b was that the former had asparagine and the latter had histidine at position 26.


Relaxation spectra of aspartate transcarbamylase. I. Interaction of 5-bromocytidine triphosphate with native enzyme and regulatory subunit.


The primary structure of the 16S and 23S ribosomal RNAs.


Studies on the enzymatic binding of aminoacyl transfer ribonucleic acid to ribosomes in a Drosophila in vitro system.


A study of the mechanism of action of E. coli ribonuclease 3.


Accessibility of 5S RNA to ribonucleases in Escherichia coli ribosomes.


Biologically active water-insoluble protein polymers. Their use for the isolation of specifically interacting proteins.


Venereal disease and the great.

Venereal disease and the great.


Dry method for estimation of mixed venous CO 2  concentration.


The Colonel is a lady--and a nurse.

The Colonel is a lady--and a nurse.


A pioneer in nursing education.

A pioneer in nursing education.


The stimulus-secretion coupling of glucose-induced insulin release. II. Interaction of alkali and alkaline earth cations.


Insulin release and glucose changes induced by serotonin.


Pharmacological studies on  -guanidinobutyramide.


Stereospecific glucose uptake by pancreatic  -cells.


Visceral lesions caused by M. leprae--a histopathological study.


Some aspects of the pathology of nerves in leprosy.


Detection of bacteriuria--a comparison of tow methods.


Introduction to the symposium on leprosy.


Comparison of tanned red cells haemagglutination test and immunofluorescent technique for the detection of autoantibodies in rheumatic fever and rheumatic heart disease.


Dr. K.C. Chaudhuri--a pen portrait of the man.


Dr. K.C. Chaudhuri. Pioneer pediatrician of Bengal.


The doyen of pediatrics. Doctor K.C. Chaudhuri--father of Indian pediatrics.


Antibody in tears following intranasal vaccination with inactivated virus. II. Enhancement of tear antibody production by the use of polyinosinic: polycytidilic acid (poly I:C).


Antibody in tears following intranasal vaccination with inactivated virus. 3. Role of tear and serum antibody in experimental vaccinia conjunctivitis.


Management and medical care of goldfish.


Purification of an Escherichia coli leucine suppressor transfer ribonucleic acid and its aminoacylation by the homologous leucyl-transfer ribonucleic acid synthetase.


Past personalities and present problems. The 1971 AANS presidential address.


Magnesium metabolism of sheep fed different levels of potassium and citric acid.


Current concepts in the management of brain trauma.


Multilating injuries of the wrist.

Multilating injuries of the wrist.


An approach to the biogenetic classification of indole alkaloids.


Plants used against cancer. A survey.

Plants used against cancer. A survey.


Humans as walking legumes.

Humans as walking legumes.


Influence of alcohol and choline deficiency upon rat liver uptake of choline.


A method for rapid implantation of several intracranial electrodes.


Effects of cortical ablations upon recovery from the septal syndrome in hooded rats.


Single cell activity in the hypothalamus in intact and adrenalectomized rats.


The identification of self-stimulation elements for adjacent electrodes in the cat.


Effect of food deprivation on hypothalamic self-stimulation in stimulus-bound eaters and non-eaters.


Self-induced drinking in rats: the qualitative identity of drive and reward systems in the lateral hypothalamus.


A new type of cannula for central administration of drugs in rats.


Modulation of hypothalamic self-stimulation and escape behavior by chlordiazepoxide.


Comparison of learning for hypothalamic and septal stimulation.


Planning of heart transplantation by means of critical path analysis.


Bullous erythema multiforme concomitant with lupus erythematosus in the light of immunofluorescent studies.


Assessment of exchangeable potassium in myasthenia and in certain other neurological diseases.


Mary Gordon, M.B., B.Sc. (Univ. Durh.).

Mary Gordon, M.B., B.Sc. (Univ. Durh.).


Modification of drug absorption by bacterial toxins. I. The influence of staphylococcal alpha toxin and Shigella dysenteriae toxin on resorption of sodium salicylate.


Modification of drug absorption by bacterial toxins. II. The influence of staphylococcal alpha toxin and Shigella dysenteriae toxin on the penetration of sodium salicylate through the placental barrier.


The use of expectorants.

The use of expectorants.


Studies in urinary tract infections. 8. Escherichia coli O:H serotypes in recurrent infections.


Changes in forelimb weight and vascular resistances during endotoxin shock.


Regulation of pyrimidine and arginine biosynthesis in mammals.


Liver distribution and uptake of molecules studied by rapid indicator-dilution technique.


Effect of hypothalamic stimulation on plasma glucose, insulin, and glucagon levels.


Measurement of intrarenal plasma flow with antiglomerular basement-membrane antibody.


Median eminence lesions and serum prolactin: influence of ovariectomy and ergocornine.


Hypothalamic stimulation and baroceptor reflex interaction on renal nerve activity.


Calcium uptake by pancreatic  -cells as measured with the aid of  45 Ca and mannitol- 3 H.


Hypolipidemic drugs and hyperlipoproteinemia.


Dental materials: from Wornercast to Worcra processes.


Recent advances in pain relief in childbirth. I. Inhalation and systemic analgesia.


Times for the analysis of stimuli and for the selection of responses.


Cardiac transplantation today.

Cardiac transplantation today.


Prevention of early postoperative deep vein thrombosis by intermittent compression of the leg during surgery. A clinical trial is described in which the effect of intermittent compression of the lower limb during surgery on the incidence of early postoperative deep vein thrombosis was assessed. Deep vein thromboses were diagnosed by the (125)I-fibrinogen uptake test. Peroperative intermittent compression was achieved by means of an inflatable plastic splint coupled to a pneumatic controller. By compressing only one leg of each patient, each patient acted as his own control.With a sequential statistical analysis, 39 patients were required to pass the 5% level of significance. Eleven thrombi were detected in the control (uncompressed) legs and two occurred in the compressed legs; one of the latter was bilateral. The investigation shows that increasing the pulsatility of the venous flow in the leg is a potent prophylactic against postoperative deep vein thrombosis.


Availability of cadaveric kidneys for transplantation. The availability of cadaveric donor kidneys for transplantation has been estimated from a retrospective survey of hospital deaths in Wales during 1969. Seventy-six "probable donors" and 167 "possible donors" were identified, indicating that the potential supply of donor kidneys is sufficient to meet the present demands of patients needing kidney transplantation.


Cerebellar and cerebral inputs to physiologically identified efferent cell groups in the red nucleus of the cat.


Splenectomy for hematologic disorders.

Splenectomy for hematologic disorders.


Fine structure of the fertilization membranes of sea urchin embryos.


Analysis of cleavage stimulus by means of micromanipulation of sea urchin eggs.


Preparation of neurons and glial cells from rat brain by zonal centrifugation.


Mass isolated ameba nuclei. I. Isolation procedure and determination of macromolecular composition and RNA polymerase activity.


Protoplasts isolated from callus cells of maize endosperm.


Photoelectric monitoring of single beating heart cells in culture.


Mechanisms of active transport in isolated membrane vesicles. II. The mechanism of energy coupling between D-lactic dehydrogenase and beta-galactoside transport in membrane preparations from Escherichia coli.


Temperature-sensitive cell division component in a mutant of Salmonella typhimurium.


Sense and nonsense in bacterial taxonomy.


Effect of gamma-radiation on the donor ability of recA and recA+ strains of Escherichia coli.


Citrobacter freundii mutants deficient in host specificity functions and their recipient ability for foreign deoxyribonucleic acid.


Dr. C.O. Karunakaran, M.B.B.S., D.P.H., D.T.M. & H. (Camb.), D.B. (Lond.).


Effects of hyperoxia on composition and rate of synthesis of fatty acids in Escherichia coli. Growth of and fatty acid synthesis in Escherichia coli were inhibited by oxygen at partial pressures above 1 atm and were prevented by exposure to oxygen at 4.2 atm on membranes incubated on a minimal medium. Growth and fatty acid synthesis returned to control rates when cells were removed from hyperoxia to air. The spectrum of fatty acids produced was unchanged by oxygen at pressures which reduced the rate of synthesis. In situ fatty acids were stable to oxygen at pressures which prevented growth and synthesis. Reinitiation of synthesis after complete inhibition in hyperoxia occurred without production of aberrant fatty acids. Fatty acid synthetase specific activity was virtually unchanged, compared with air controls, in cells exposed either to 3.2 or to 15.2 atm of oxygen. The spectrum of fatty acids synthesized by cell-free extracts during incubation in 4.2 atm of oxygen was not different from air-incubated controls. Synthetase assays included added NADPH, acyl carrier protein, mercaptoethanol, and malonyl coenzyme A; hence, damage, other than reversible sulfhydryl oxidation, to the apoenzymes of synthetase was ruled out.


23 years of stereotaxic neurosurgery.

23 years of stereotaxic neurosurgery.


The effect of drugs on hyperactivity in children with some observations of changes in mineral metabolism.


Total synthesis of steroids and novel 13-alkylgonane derivatives including norgestrel.


Metabolism of mouse embryos.

Metabolism of mouse embryos.


Long-time observations on the Blalock-Taussig operation. I. Results of first operation.


Screening techniques for undue lead exposure in children: biological and practical considerations.


Communication theory and its uses for medical sociology. A selected bibliography.


Further studies on the enzymatic and chemical inactivation of hypothalamic FSH-RH.


Some letters from the Herrick-Lashley correspondence.


Dyslexia in relation to cerebral dominance and temporal analysis.


Patterns of drawing disability in right and left hemispheric patients.


Moral issues in hemodialysis and renal transplantation.


Dermo-epidermal interactions.

Dermo-epidermal interactions.


The influence of nutrition on the DNA content of Escherichia coli and its response to ultraviolet light.


Effects of sensitized and unsensitized longwave U.V.-irradiation on the solution properties of DNA.


Uridine diphosphate-4-keto-glucose, an intermediate in the uridine diphosphate-galactose-4-epimerase reaction. When UDP-galactose 4-epimerase (EC 5.1.3.2) from Escherichia coli is incubated with UDP-galactose, then reduced with NaB(3)H(4), label is found in UDP-glucose and UDP-galactose. Enzymatic and chemical degradation demonstrates that the label is bound to carbon 4 of the glycosyl moieties. These results provide direct evidence for the existence of UDP-4-keto-glucose as an enzyme-bound intermediate in the epimerization reaction, and they exclude the formation of a 3-keto intermediate.


Host-gene control of C-type tumor virus-expression and tumorigenesis: relevance of studies in inbred mice to cancer in man and other species. C-type RNA viruses are tumorigenic in mice and other species. They are widespread and transmitted in an intimate relationship with host-cell genetic material. Tumorigenesis is no doubt a complex biological phenomenon, and involves both genetic and environmental factors. Among the genetic factors, single-locus determination is probably exceptional; yet, a number of genes are known in inbred mice and other species, including man, that can exert a major influence. Clearly, studies with inbred mice apply outside the murine model and justify generalizations on the etiology of cancer. The types of cancer observed in man are similar to those of inbred strains of mice, and their stochastic and often familial occurrences suggest that genetic factors play important roles in both man and mouse. A number of heritable disorders predipsose to tumorigenesis. Investigations of the relationship between various polymorphic phenotypes and human tumors should be intensified. For instance, a study of high-risk human-tumor groups could aid considerably not only in the recognition of etiological agents but also of underlying host-gene controlled mechanisms. Indeed epidemiological studies have already led to the identification of mutant genes that influence the incidence of human cancer. Such studies may be guided by findings in inbred strains of mice. There is good evidence in inbred mice that mutations in host chromosomes determine whether or not genes coding for RNA oncogene viral "functions" become expressed as cancer. Although an etiological role of C-type RNA virus has not yet been demonstrated in human cancer, studies of the interaction of viral and host-cell genomes in mice and other species, and the visualization of C-type virus in human tumors have provided good reasons for suspecting the presence in man of oncogenic genes (oncogenes) homologous to those of the C-type viral genomes, which are well studied in mice, hamsters, cats, rats, and chickens.


The significance of the evidence about ascorbic acid and the common cold. Only four independent double-blind studies have been reported of the effect of ascorbic acid regularly ingested in daily amounts more than 100 mg, in comparison with a placebo, in decreasing the incidence and integrated morbidity of the common cold for subjects exposed to cold viruses in the ordinary way and without colds when the test period began. A statistical analysis of these four studies leads to rejection of the null hypothesis that ascorbic acid has no more protective power than the placebo at the 99.86% level of confidence for the incidence of colds and the 99.9978% level of confidence for the integrated morbidity.


Bifunctional inhibitors of pepsin.

Bifunctional inhibitors of pepsin. Two bifunctional reagents designed to probe the active site of pepsin and other acid proteinases are described. One of these, the bisdiazoketone 1,1-bis(diazoacetyl)-2-phenylethane inactivates pepsin at pH 5.0 much more rapidly than the corresponding monodiazoketon 1-diazoacetyl-2-phenylethane, whereas the other, the bromodiazoketone dl-1-diazoacetyl-1-bromo-2-phenylethane is less effective in this regard. The inactivation is greatly accelerated by the presence of Cu(II), and the pH dependence of the process is consistent with the interaction of the enzyme with the metal complex of the carbene derived from the reagent. The bisdiazoketone appears to react stoichiometrically with pepsin in a 1:1 ratio to form a product whose apparent molecular size is the same as that of untreated pepsin. The inactivation of pepsin by the bromodiazoketone is accompanied by the release of stoichiometric amounts of bromide ions and the formation of a major product whose apparent size is similar to that of pepsin, and a minor component larger than the untreated enzyme.


Association factor of ribosomal subunits from Bacillus stearothermophilus. The isolation of a new factor, which can cause the in vitro association of 30S and 50S ribosomal subunits at low Mg(++) concentration, is described. The association factor is eluted together with the dissociation protein when ribosomes of Bacillus stearothermophilus are washed with salt solutions of high concentration. The association activity is heat-stable, whereas dissociation factor is inactivated after 10 min at 80 degrees C. This treatment allows the separation of both factors. Several properties rule out the possibility that uncharged, amino-acyl-, or peptidyl-tRNA are responsible for the association process described in this report. Digestion with trypsin shows that the association factor contains at least two components, one of which is a protein.