Criteria for examining the immune response to infectious bronchitis virus.


Evidence of a sire effect on piglet mortality.


An approach to identifying and maintaining salmonella-free chickens.


A sequential trial of amylobarbitone sodium used as sedation for confused female psychogeriatric patients.


The influence of endotoxin on the response of chickens to Plasmodium lophurae malaria.


Culture of shell and shell membranes for efficient isolation of Arizona from turkey hatching eggs.


Heinz Hartmann 1894-1970..

Heinz Hartmann 1894-1970..


Herman Nunberg 1884-1970.

Herman Nunberg 1884-1970.


Effectiveness of various tranquilisers in the management of senile restlessness.


An overview of Heinz Hartmann's contributions to psychoanalysis.


Freud in Paris: a crucial stage.

Freud in Paris: a crucial stage.


The induction of hair follicle formation in the adult hooded rat by vibrissa dermal papillae.


For better or for worse? Psychotherapy and variance change: a critical review of the literature.


Diazepam in the management of heroin withdrawal in adolescents: preliminary report.


Neonatal myasthenia gravis and antimuscle antibodies.


Vasopressin injection in cervical conization. A double-blind study.


Measurements of effective pulmonary blood flow in the normal newborn human infant.


Anencephaly and spina bifida in Israel.

Anencephaly and spina bifida in Israel.


Renal homotransplantation in pediatric patients.


Neonatal pneumopericardium.

Neonatal pneumopericardium.


Detection of Rift Valley fever virus by the fluorescent antibody technique in organs of experimentally infected animals.


Platelet aggregation induced by basement membranes.


Lipids in membrane development.

Lipids in membrane development.


Cholesterol turnover in man.

Cholesterol turnover in man.


Hypnosis: its reascence as a treatment modality.


New look at arrhythmias.

New look at arrhythmias.


The blood flow in tubed skin pedicles: calibration for the photoelectric plethysmograph.


The early ambulation of patients with lower limb grafts.


The treatment of split thickness skin graft donor sites using n-butyl and n-heptyl 2-cyanoacrylate.


Progressive facial hemiatrophy evaluation of treatment in a case after nine years.


Maxillary osteotomies and bone grafts for correction of contoural and occlusal deformities.


The role of the lip adhesion procedure in cleft lip repair.


Library services.

Library services.


Management.

Management.


Management engineering.

Management engineering.


Manpower.

Manpower.


Patient care evaluation.

Patient care evaluation.


Pharmacy.

Pharmacy.


Planning.

Planning.


Safety.

Safety.


Auxiliaries and volunteers.

Auxiliaries and volunteers.


Education.

Education.


Environmental services.

Environmental services.


Financing.

Financing.


Hospital law.

Hospital law.


Information processing.

Information processing.


A dentist's award-winning avocation--creative photography.


Rapid preparation of thick sections of plastic-embedded undecalcified bone for microscopicxaminationwith reflected light.


Effect of diethylstilbestrol implantation on testicular development in the boar.


Effects of molybdate and selenite on formate and nitrate metabolism in Escherichia coli. The effects of adding molybdate and selenite to a glucose-minimal salts medium on the formation of enzymes involved in the anaerobic metabolism of formate and nitrate in Escherichia coli have been studied. When cells were grown anaerobically in the presence of nitrate, molybdate stimulated the formation of nitrate reductase and a b-type cytochrome, resulting in cells that had the capacity for active nitrate reduction in the absence of formate dehydrogenase. Under the same conditions, selenite in addition to molybdate was required for forming the enzyme system which permits formate to serve as an effective electron donor for nitrate reduction. When cells were grown anaerobically on a glucose-minimal salts medium without nitrate, active hydrogen production from formate as well as formate dehydrogenase activity depended on the presence of both selenite and molybdate. The effects of these metals on the formation of formate dehydrogenase was blocked by chloramphenicol, suggesting that protein synthesis is required for the increases observed. It is proposed that the same formate dehydrogenase is involved in nitrate reduction, hydrogen production, and in aerobic formate oxidation.


Mechanism of D-cycloserine action: transport mutants for D-alanine, D-cycloserine, and glycine. The accumulation of d-alanine and the accumulation of glycine in Escherichia coli are related and appear to be separate from the transport of l-alanine. The analysis of four d-cycloserine-resistant mutants provides additional support for this conclusion. The first-step mutant from E. coli K-12 that is resistant to d-cycloserine was characterized by the loss of the high-affinity line segment of the d-alanine-glycine transport system in the Lineweaver-Burk plot. This mutation, which is linked to the met(1) locus, also resulted in the loss of the ability to transport d-cycloserine. The second-step mutation that is located 0.5 min from the first-step mutation resulted in the loss of the low-affinity line segment for the d-alanine-glycine transport system. The transport of l-alanine was decreased only 20 to 30% in each of these mutants. A multistep mutant from E. coli W that is 80-fold resistant to d-cycloserine lost >90% of the transport activity for d-alanine and glycine, whereas 75% of the transport activity for l-alanine was retained. E. coli W could utilize either d- or l-alanine as a carbon source, whereas the multistep mutant could only utilize l-alanine. Thus, a functioning transport system for d-alanine and glycine is required for both d-cycloserine action and growth on d-alanine.


Influence of temperature on the iron metabolism of a fluorescent pseudomonad. The iron requirement for maximal cell yields of a fluorescent pseudomonad increases as the temperature of incubation is increased. On a succinate salts medium, maximal cell yields are attained at iron concentrations of 0.10 mug/ml of added iron at 20 C and at 3.0 mug/ml of added iron at 28 C. This bacterium does not grow in the basal medium at 31 C even in the presence of 0.01 to 10 mug/ml of added iron. The inability to grow at the higher temperature is due to the loss, by this organism, of its ability to biosynthesize hydroxamate iron transport compounds at temperatures of 28 C and above, since supplementation with such compounds produced by this organism at lower temperatures promoted growth at 31 C. The biosynthesis of these compounds at lower temperatures contributes to the efficient utilization of iron by the bacterium.


Biochemical studies of melibiose metabolism in wild type and mel mutant strains of Salmonella typhimurium. I identified two enzyme activities, alpha-galactosidase and a galactoside permease, required for melibiose metabolism by Salmonella typhimurium. These activities are very low under normal growth conditions, but their production can be induced by melibiose and gratuitously by melibiitol. Melibiose induction is severely inhibited by glucose, but the glucose effect can be countered by 3', 5' cyclic adenosine monophosphate. I isolated two phenotypic classes of mutants not able to utilize melibiose as a carbon source. One class, Car(-), is deficient in the phosphotransferase system. The other, Mel, lacks either alpha-galactosidase, galactoside permease, or both functions.


Nalidixic acd-resistant mutants of Escherichia coli deficient in isocitrate dehydrogenase. icd Mutants of Escherichia coli K-12, selected for their resistance to nalidixic acid, are deficient in isocitrate dehydrogenase.


Biosynthesis of T1 antigen in Salmonella: origin of D-galactofuranose and D-ribofuranose residues. The "T1 side chain" portion of cell wall lipopolysaccharide from T1 strains of Salmonella contains d-galactofuranose and d-ribofuranose residues. Isotope labeling studies, using intact cells of mutants each blocked at either of the two different steps of d-galactose metabolism (uridine diphosphate-glucose 4-epimerase and galactose-1-P uridylyl transferase) or at phosphoglucoisomerase, led to the following conclusions. (i) d-Galactofuranose residues are synthesized from d-galactopyranose or its derivatives, rather than by a direct conversion from other hexopyranoses or their derivatives. (ii) The pyranose-to-furanose conversion does not appear to take place at the level of the free d-galactose or d-galactose 1-phosphate. This result suggests that the conversion may occur at the stage of uridine diphosphate-d-galactose. (iii) In a mutant lacking phosphoglucoisomerase, d-ribofuranose residues in T1 side chains contained (14)C derived from exogenous d-fructose-U-(14)C, but little (3)H from exogenous d-glucose-1-(3)H. Thus, no evidence was found for a direct pathway of aldohexose-to-ribose conversion involving a loss of one of the carbons in the C2-C6 moiety of aldohexoses. This suggests, but does not prove, that the T1 ribofuranose residues are synthesized by conventional mechanisms involving hexose monophosphate shunt and transketolase-transaldolase reactions.


Biosynthesis during recovery of heat-injured Salmonella typhimurium. Salmonella typhimurium 7136 incorporated label from glucose-U-(14)C into nucleic acids, lipids, and pool material during recovery from heat injury. There was very little incorporation of label into protein during recovery.


Studies on Escherichia coli sex factors: evidence that covalent circles exist within cells and the general problem of isolation of covalent circles. We examined in detail conditions necessary for making reproducible and for maximizing the amount of deoxyribonucleic acid obtained from a sex factor-containing cell as covalent circles. The results argue that under optimal conditions covalent circles are neither created nor lost during the isolation procedure. The causes of the culture-to-culture variation in recovery of covalent circular deoxyribonucleic acid were investigated but an understanding of this is not yet at hand. Some commonly used conditions which drastically reduce the recovery of covalent circles are described.


Influence of thymine starvation on the integrity of deoxyribonucleic acid in Escherichia coli. The influence of thymine starvation on the single-strand molecular weight of deoxyribonucleic acid (DNA) from Escherichia coli was determined by sedimentation through gradients of alkaline sucrose. Growth of cells for as long as 150 min in thymineless medium did not significantly reduce the molecular weight below the control value of 2.4 +/- 0.3 x 10(8) daltons. Incubation of cells in thymineless medium after exposure to 500 ergs/mm(2) of ultraviolet light or 20 krad of (137)Cs gamma rays did not appear to block the rejoining of single-strand breaks associated with irradiation. Thus, DNA repair enzymes, presumably including DNA ligase, are not significantly inhibited by thymine starvation.


Biosynthesis of biotin: synthesis of 7,8-diaminopelargonic acid in cell-free extracts of Escherichia coli. Cell-free extracts prepared from a biotin auxotroph of Escherichia coli were active in catalyzing the synthesis of 7,8-diaminopelargonic acid, an intermediate of the biotin pathway, from 7-oxo-8-aminopelargonic acid. The product was identified on the basis of its chromatographic characteristics and its biotin activities for biotin auxotrophs of E. coli. Enzyme activity was determined in a reaction coupled with the desthiobiotin synthetase system, which is required for the conversion of 7,8-diaminopelargonic acid to desthiobiotin, and by measuring the amount of desthiobiotin formed by microbiological assay. The reaction was stimulated by l-methionine and pyridoxal-5'-phosphate. l-Methionine could not be replaced by any other amino acids tested. Pyridoxamine and pyridoxamine-5'-phosphate were as active as pyridoxal phosphate. The enzyme, presumably an aminotransferase, was demonstrable in the parent strain of E. coli and all mutant strains tested with the exception of a strain which is able to grow on diaminopelargonic acid but not on 7-oxo-8-aminopelargonic acid. Furthermore, the enzyme was repressible by biotin. The results were consistent with the hypothesis that the biosynthesis of 7,8-diaminopelargonic acid from 7-oxo-8-aminopelargonic acid is an obligatory step in the biosynthetic pathway of biotin in E. coli.


Division cycle of Myxococcus xanthus. 3. Kinetics of cell growth and protein synthesis. The kinetics of cell growth and protein synthesis during the division cycle of Myxococcus xanthus was determined. The distribution of cell size for both septated and nonseptated bacteria was obtained by direct measurement of the lengths of 8,000 cells. The Collins-Richmond equation was modified to consider bacterial growth in two phases: growth and division. From the derived equation, the growth rate of individual cells was computed as a function of size. Nondividing cells (growth phase) comprised 91% of the population and took up 87% of the time of the division cycle. The absolute and specific growth rates of nondividing cells were observed to increase continually throughout the growth phase; the growth rate of dividing cells could not be determined accurately by this technique because of changes in the geometry of cells between the time of septation and physical separation. The rate of protein synthesis during the division cycle was measured by pulselabeling an exponential-phase culture with radio-active valine or arginine and then preparing the cells for quantitative autoradiography. By measuring the size of individual cells as well as the number of grains, the rate of protein synthesis as a function of cell size was obtained. Nondividing cells showed an increase in both the absolute and specific rates of protein synthesis throughout the growth phase; the specific rate of protein synthesis for dividing cells was low when compared to growthphase cells. Cell growth and protein synthesis are compared to the previously reported kinetics of deoxyribonucleic acid and ribonucleic acid synthesis during the division cycle.


Genetic analysis of diaminopimelic acid- and lysine-requiring mutants of Escherichia coli. Several diaminopimelic acid (DAP)- and lysine-requiring mutants of Escherichia coli were isolated and studied by genetic, physiological, and biochemical means. The genes concerned with DAP-lysine synthesis map at several different sites on the E. coli chromosome and, therefore, do not constitute a single operon. Three separate loci affecting DAP synthesis are located in the 0 to 2.5 min region of the genetic map. The order of the loci in this region is thr-dapB-pyrA-ara-leu-pan-dapC-tonA-dapD. Two additional DAP genes map in the region between min 47 and 48, with the gene order being gua-dapA-dapE-ctr. The lys locus at min 55 determines the synthesis of the enzyme DAP decarboxylase, which catalyzes the conversion of DAP into lysine. The order of the genes in this region is serA-lysA-thyA.


Chromoome mapping of asteurella pseudotuberculosis by interrupted mating. Pasteurella pseudotuberculosis, containing the Escherichia coli plasmid F'lac, transferred its chromosome in an oriented manner to each of five multiply auxotrophic strains of P. pseudotuberculosis. In a mating system containing gelatin, glucose, and phosphate buffer, a maximum of 0.02% of the donor cells transferred lead markers. The donor population was counterselected with nalidixic acid. We established the entry time of seven markers as follows: proline (11 min); arginine (14 min); histidine (14 min); threonine (25 min); lysine (50 min); tyrosine (67 min); and tryptophan (77 min). However, an analysis of the inheritance of unselected markers did not support the simplest assumption that the chromosome was transferred as Origin... pro... arg his... thr... lys... tyr... trp.... The markers common to all five recipients, arg and his, were closely linked, but of the five other markers, each unique to a different recipient strain, only trp was linked to arg and his. Our data suggest that the Pasteurella chromosome is transferred in more than one linkage group.


Chromosome transfer and recombinant formation with deoxyribonucleic acid temperature-sensitive strains of Escherichia coli. Haploid recombinant yield is reduced in matings conducted at 42.5 C between deoxyribonucleic acid (DNA) temperature-sensitive [dna(-)(TS)] recipients unable to synthesize DNA at 42.5 C and any of the three major donor types (Hfr, F(+), F') of Escherichia coli. No such reduction is observed in matings conducted at 42.5 C when the dna(-)(TS) mutation is in the donor parent. Evidence is presented which indicates that chromosome transfer from donors to recipients unable to replicate DNA at 42.5 C during vegetative growth occurs at normal frequencies when the mating is conducted at 42.5 C. It is concluded that some stage in haploid recombinant formation is adversely affected in dna(-)(TS) recipients mated at the temperature restrictive for DNA synthesis.


Effect of nalidixic acid on semionservative replication and repair synthesis after ultraviolet irradiation in Escherichia coli. Both semiconservative deoxyribonucleic acid replication and "extensive repair" synthesis, after ultraviolet irradiation, appear to be blocked by nalidixic acid. These findings suggest that the agent(s) responsible for both of these modes of replication, or some necessary common process or structure, is affected by this drug.


Lysine suppressor in Escherichia coli.

Lysine suppressor in Escherichia coli. Head amber mutant of phage T4D was used to determine the efficiency of suppression and the amino acid inserted by Escherichia coli CA273 (sup-273 formerly Su(+) (beta)). The level of suppression determined was 8%, and the amino acid inserted was shown to be lysine.


Role of lipopolysaccharides in antibiotic resistance and bacteriophage adsorption of Escherichia coli K-12. Novobiocin-supersensitive (NS) mutants which could not grow on plates containing 40 mug or less of novobiocin per ml were isolated from Escherichia coli strain JE1011 (derived from E. coli K-12). Most of these NS mutants were found to have incomplete lipopolysaccharides (LPS), and they lack phosphate diester bridges in their backbone structure, with or without total loss of heptose, to which the phosphate diester is linked, and consequently lack external outer-core oligosaccharides. The phosphate diester bridges in the LPS backbone are apparently very important in forming a cell surface structure resistant to the penetration of antibiotics such as novobiocin, spiramycin, and actinomycin D. NS mutants, with incomplete LPS, lacking phosphates in their backbone structure were found to be resistant to phage T4, and those which also lacked heptose were resistant to phages T4 and T7. In contrast to the generally accepted idea that resistances to phages T3, T4, and T7 are linked genetically, no NS mutant was found to be resistant to T3. The possible structures of the receptors for T4 and T7 are discussed. The positions of novobiocin-supersensitive genes on the chromosome of several of the NS mutants defective in LPS were mapped. The genes were designated lpcA (between ara and lac) and lpcB (between 55 min and 60 min). The latter seemed to be a group of several related genes.


Mutants of Escherichia coli with a growth requirement for either lysine or pyridoxine. Two distinct phenotypic classes of lysine requiring auxotrophs of Escherichia coli are described. Mutants of the LysA class produce little or no active diaminopimelic acid (DAP) decarboxylase and specifically require lysine for growth. Mutants of the LysB class produce a cryptic DAP decarboxylase which can be activated both in vivo and in vitro by higher than normal levels of its cofactor, pyridoxal 5'-phosphate. The LysB mutants have an alternate requirement for lysine or pyridoxine. Both LysA and LysB mutations map at 55 min, close to the thyA locus of E. coli. The association between pyridoxal phosphate and DAP decarboxylase appears to be much weaker in LysB mutants than in wild-type bacteria, and the mutant enzyme also sediments more slowly than wild-type enzyme in sucrose density gradients. The results suggest that the LysB mutations alter a specific region (or subunit) of the enzyme molecule which is needed to stabilize the binding of pyridoxal phosphate. These studies help to resolve certain contradictory observations on DAP decarboxylase reported earlier and may have relevance to pyridoxal phosphate enzymes in general. Prototrophic revertants of LysB mutants arise by second site mutations that result in increased availability of intracellular pyridoxal phosphate. These revertants appear to be derepressed for pyridoxine biosynthesis.


A histopathological study of tissue responses to the pulsar pulsating water pressure cleansing device.


The role of classification in the effective management of proximal pockets adjacent to edentulous areas.


Retraction hooks, suture covers and dressing retainers for flap procedures.


Effect of gastric peptides on acid and pepsin secretion in the frog Discoglossus pictus.


Time required in rubber dam placement.

Time required in rubber dam placement.


Reminiscences of the Physiological Laboratory at Cambridge in 1909-10.


Model for chemotaxis.

Model for chemotaxis.


Effectiveness of acetic acid and chromic anhydride in prevention of dental caries.


Pulpal responses to cavity drying.

Pulpal responses to cavity drying.


Traveling bands of chemotactic bacteria: a theoretical analysis.


Hypothesis for catabolite repression.

Hypothesis for catabolite repression.


The legal and medical aspects of the trial and death of Christ.


Fractured permanent incisors in a clinic population.


The Bible and legal medicine.

The Bible and legal medicine.


Galactosemia screening of newborns in Massachusetts.


Bundy against herbicides.

Bundy against herbicides.


Human prolactin.

Human prolactin.


Polymorphism of human enzyme proteins.

Polymorphism of human enzyme proteins.


Extensions of the allosteric model for haemoglobin.


Late DNA replication in the paternally derived X chromosome of female kangaroos.


New method of collagen extraction for radiocarbon dating.


Dietary migraine and tyramine metabolism.


Identification of prostaglandin F2-alpha in the utero-ovarian blood of guinea-pig after treatment with oestrogen.


Transmission of double stranded RNA viruses to a strain of Penicillium stoloniferum through heterokaryosis.


Heavy metals and the fertilization of rainbow trout eggs.


Addressed exponential delay line theory of cochlear organization.


Cyanide insensitive culture form of Trypanosoma brucei.


Role of predators in the reversal of imbalances in microbial ecosystems.


Advancement along a complexity gradient in perceptual level and brain damage.


Use of the "Famous Sayings" test in South Africa.


Figure drawings by sex offenders and a random sample of offenders.


Objective versus individual mental ability tests with former Head Start children in the first grade.


Cognitive style, attention, and learning.


Differences in the size of drawings of an important symbol as a function of level of conceptual system functioning.


Uropepsinogen reaction in surgical stress in terms of age.


Factors influencing distribution of some proteolytic enzymes in soils.


Treatment of nocturnal enuresis with imipramine and related preparations. A double blind trial with a placebo.


Imipramine treatment of enuresis. A material from Christmas Seal Homes.


Uricult. A simple method of semi-quantitative culture from urine.


The effect of hyperbaric oxygenation upon renal hemodynamics.


Improved immediate function of experimental cadaver renal allografts by elimination of agonal vaospasm.


Preliminary observations on infective diarrhoea. II. Bacteria.


Further observations on the digestive proteolytic enzymes in ascariasis.


Some physiologic responses to high-speed handpiece noises.


Dental-medical team helps to revise Egyptian history.


Extracoronal attachment design for removable partial dentures on contoured abutment retainers.


Personalized impression trays. 2.

Personalized impression trays. 2.


Baltimore College of Dental Surgery. First dental school in the world. Established 1839.


The role of the exocrine pancreas in the stimulation of insulin secretion by intestinal hormones. I. The effect of pancreozymin, secretin, gastrin-pentapeptide and of glucagon upon insulin secretion of isolated islets of rat pancreas.


Immune factors in corneal graft rejection.


Peroxidase diffusion in the normal and photocoagulated retina.


The origin and movements of the hepatogenic cells in the chick embryo as determined by radioautographic mapping.


Hereditary hemorrhagic telangiectasia (Osler-Weber-Rendu disease): discussion and report of case.


Hormones and resistance.

Hormones and resistance.


Synthesis and characterization of some 3-nitroso-2-arylimidazo(1,2-a)pyrimidines.


Vagal adrenergic degranulation of enterochromaffin cell system in guinea pig duodenum.


Rabies in a child diagnosed by a new intra-vitam method. The cornea test.


A few curious and interesting facts concerning the tooth.


Historical development of medicine in Brooklyn.


Ignace Guillotin, M.D. (1738-1814).

Ignace Guillotin, M.D. (1738-1814).


Influence of reserpine and dihydrazinophthalazine on cerebrospinal fluid and blood catecholamines in the hypertensive disease.


Microvasculature and loose connective tissue in freeze-fracture peparations.


Psychological evaluation of intelligence and personality in parkinsonism before and after stereotaxic surgery.


The Philadelphia story of optometry.

The Philadelphia story of optometry.


Screw retained telescopic prostheses.

Screw retained telescopic prostheses.


The foot engine. A centenary.

The foot engine. A centenary.


The Royal Army Dental Corps.

The Royal Army Dental Corps.


Clinical research in general practice.

Clinical research in general practice.


Effect of irradiation temperature in the range--196 to 95C on the resistance of spores of Clostridium botulinum 33A in cooked beef.


Particle size, water-stable aggregates, and bacterial populations in lake sediments.


Iron uptake in Salmonella typhimurium.

Iron uptake in Salmonella typhimurium.


The ultrastructure of Crithidia fasciculata. A freeze-etching study.


Enzyme cytochemistry and autoradiography of adenovirus-infected cells as determined with the electron microscope.


Ultrastructural changes in tissues of larval Elateridae (Coleoptera) infected with the fungus Metarrhizium anisopliae.


Effects of L-asparagine deprivation on the cell cycle of the Jensen sarcoma.


Amino acids of the 6C3HED lymphosarcoma following treatment with asparaginase.


Influence of altitude on late effects of radiation in RF-Un mice: observations on survival time, blood changes, body weight, and incidence of neoplasms.


Impacted teeth.

Impacted teeth.


Preprosthetic surgery.

Preprosthetic surgery.


Biosynthesis of plasma glycoproteins.

Biosynthesis of plasma glycoproteins.


The biosynthesis of hyaluronic acid by group A streptococcus.


Acylation of amino-sugar intermediates and the regulation of epithelial glycoprotein biosynthesis.


Trends in clinical pathologists.

Trends in clinical pathologists.


A two-dimensional chromato-bioautographic method for complete separation of individual plasma cobalamins.


Fatal infectious mononucleosis: a critical review.


Hemangioblastoma: a vascular odontogenic tumor.


The reappearance of malaria in the USA.

The reappearance of malaria in the USA.


Experimental evaluation of haemodynamic effects of inferior vena cava ligation.


Direct measurement of unilateral renal blood flow by dye dilution method.


Skin and muscle celarance with 133-xenon and 125-iodine antipyrine. Effect of vascular reconstruction examined.


A projective cartoon investigation of nurse-patient psychodramatic role perception and expectation.


Bacteriuria and urinary tract infection in infancy and childhood. A review.


Congenital malformations: from Hippocrates to thalidomide.


Cultivation methods for the detection of aerobic sporeforming bacteria.


New direct bonding system for plastic brackets.


Is it necessary to excise the duodenal ulcer with a Billroth I anastomosis?


Infant mortality from spina bifida, congenitalhydrocephalus, monstrosity, and congenital dseases of the cardiovascular system in England and Wales.


William Sydney Charles Copeman C.B.E., T.D., M.A., M.D., F.R.C.P., 1900-1970.


Thomas Francis, Jr., MD, 1900-1969.

Thomas Francis, Jr., MD, 1900-1969.


Thomas Francis, Jr.: an appreciation.

Thomas Francis, Jr.: an appreciation.


Surface structure of articular cartilage. Historical review.


Bibliography.

Bibliography.


The London ambulance service. An historical sketch.


Thomas Francis, Jr., MD, as a clinician--1900-1969.


Thomas Francis, Jr., MD: epidemiologist to the military.


The "Undiscovered" discovery.

The "Undiscovered" discovery.


Contribution to the work of the Atomic Bomb Casuality Commission (ABCC) (Francis T).


Origins and development of the commission on influenza.


The restless spirit of Thomas Francis, Jr., still lives: the unsolved problems of recurrent influenza epidemics.


Control of influenza: lack of knowledge versus lack of application of knowledge.


Influenza 1970: unquestioned answers and unanswered questions.


Globulin fractions separated from antipertussis rabbit serum.


Molluscum contagiosum.

Molluscum contagiosum.


Editorial. Surfactant.

Editorial. Surfactant.


The effect of pretreatment of skin on artificially induced miliaria rubra and hypohidrosis.


The skin and vaccination against smallpox.


Lymphocyte function.

Lymphocyte function.


The voluntary food intake of pregnant and lactating ruminants: a review.


Studies on the isolated perfused pig liver.


Effects of long-acting thyroid stimulator on Escherichia coli.


An experimental model of active immunotherapy preceded by cytoreductive chemotherapy.


Modulation of laryngeal activity by pulmonary changes during vocalization in cats.


Effect of CIBA 13,437-Su on serum cholesterol and triglycerides, plasma fibrinogen, fibrinolysis and platelet adhesiveness in patient with ischemic hert disease.


Echinococcus granulosus and E. multilocularis: soluble antigen fluorescent antibody test.


Effect of cigarette smoking on human gastric secretory responses. The effect of smoking three cigarettes over a one-hour period on the pentagastrinstimulated submaximal plateau of acid and pepsin secretion was observed in 12 healthy human volunteers comprising men and women and smokers and non-smokers. No significant overall change in secretion was observed. In one subject, however, acid secretion was significantly inhibited (p < 0.01) and in one individual significant late stimulation (p < 0.01) of both acid and pepsin was observed.


An improved assay for haemolytic plaque-forming cells.


Informal educators for practicing physicians.


The survival of colicine-sensitive bacteria when grown with colicine-producing bacteria.


DNA replication in vivo by polynucleotide-ligase defective mutants of T4. II. Effect of chloramphenicol and mutations in other genes.


Formation of 61 s and 70 s particles from ribosomal subunits of Escherichia coli.


Electron microscopy of DNA polymerase bound to DNA.


Electron and light microscope studies of the oocyst walls, sporocysts, and excysting sporozoites of Eimeria callospermophili and E. larimerensis.


Isolation of double-helical regions rich in adenine-thymine base pairing from bacteriophage f1 DNA.


Stimulation of motility in merozoites of five Eimeria species by bile salts.


The monoxenic cultivation of Hymenolepis diminuta cysticercoids with rat fibroblasts.


In memoriam. Justin M. Andrews (1902-67).


Relining complete dentures with minimum occlusal error.


Retention of complete dentures lined with soft-curing resins.


Removable partial denture abutment tooth movement as affected by inclincation of residual ridges and type of loading.


An acrylic resin pattern for a cast dowel and core.


The effects of maternal pre-immunization on pregnancy in the mouse.


A comparative study of coital physiology, with special reference to the sexual climax.


Release of pressor substances from infarcted kidneys in rats. I. Effect of chronic administration of hydralazine.


Release of pressor substances from infarcted kidneys in rats. II. No protective action of infarcted kidneys against hemorrhagic shock.


Lipoma of the oral cavity.

Lipoma of the oral cavity.


A reciprocating brace with polyplanar hip hinges used on spina bifida children.


Use of the reciprocating brace with polyplanar hip hinge on spina bifida children.


Great school doctors and the evolution of adolescent medicine.


Marrow-derived thymus precursor cells. Availability and potential for reseeding bone marrow.


Herbert M. Stauffer, M.D. 1914-1970.

Herbert M. Stauffer, M.D. 1914-1970.


David Steel, M.D. 1893-1970.

David Steel, M.D. 1893-1970.


Lawrence Frederick Fisher M.D. 1890-1969.


3. Malaria.

3. Malaria.


A field trial of the fluorescence antibody test for toxoplasmosis in the diagnosis of ovine abortion.


Group A Streptococcus.

Group A Streptococcus.


Primary immunization of infants and children with group A streptococcal M protein.


Ultrastructural and histopathologic observations of a human cardiac transplant with features of rejection.


A new method for detecting indole in non-lactose fermenters.


Fluorescent antibody tests for syphilis using cerebrospinal fluid: clinical correlation in 150 cases.


Introduction of Caldwell Lecturer, 1970.


Drug interference with laboratory blood chemistry determinations.


Roentgenologic aspects of cardiac transplantation. Postoperative pulmonary infections.


Herbert Milton Stauffer, M.D. 1914-1970.


A general method to study changes in fluid distribution.


A general method to compare the activity of infusion fluids.


The number of patients required in a clinical trial.


Candidiasis of the oesophagus.

Candidiasis of the oesophagus.


Experimental animal results relating to time-dose relationships in radiotherapy and the "ret" concept.


Lymphangiographic demonstration of impression upon the duodenum by retriperitoneal lymph nodes.


Fourth Progress Report on the British Institute of Radiology fractionation study on 3F-week or 5F-week treatment of larynx and pharynx.


Special cardiac equipment.

Special cardiac equipment.


Logic in radiotherapeutic trials.

Logic in radiotherapeutic trials.


Mechanisms of transient cerebral ischaemia.


Myelomatosis: comparison of melphalan and cyclophosphamide therapy. UNTREATED PATIENTS SUFFERING FROM MYELOMATOSIS WERE ALLOCATED AT RANDOM FOR TREATMENT BY THE DAILY ORAL ADMINISTRATION OF EITHER CYCLOPHOSPHAMIDE OR MELPHALAN: 141 received cyclophosphamide and 133 melphalan. The trial began on 1 October 1964 and the intake of patients continued until 31 July 1968. The statistical analysis includes follow-up of the surviving patients to 31 May 1970.The most important single factor affecting the prognosis was the blood urea concentration at presentation. The median survival of the 125 patients whose blood urea concentration was less than 40 mg/100 ml was 33 months, compared with 20 months for the 96 patients whose blood urea concentration was 40-79 mg/100 ml and two months for the 55 patients whose blood urea concentration was 80 mg/100 ml or more.The median survival periods of the 114 patients in the cyclophosphamide group and of the 105 in the melphalan group whose blood urea concentration at presentation was less than 80 mg/100 ml were 27 and 23 months respectively. The difference is not statistically significant.


Ulcerative colitis. Epidemiology and aetiology, course and prognosis.


Vitamin C and the common cold.

Vitamin C and the common cold.


Surgical treatment.

Surgical treatment.


Inoculation leprosy subsequent to small-pox vaccination.


Comparative study of fluorescent labelled antibodies.


A controlled trial of vitamin E therapy in infancy.


Management of shock in acute myocardial infarction.


The relationshipbetween smoking and health: a review.


Studies on aging in a bdelloid rotifer. I. The effect of various culture systems on longevity and fecundity.


A case of human rabies in Kansas: epidemiologic, clinical, and laboratory considerations.


Paul A. Bunn, M.D., 1914-1970.

Paul A. Bunn, M.D., 1914-1970.


Morton Hamburger, M.D., 1907-1970.

Morton Hamburger, M.D., 1907-1970.


Polarity induced by chloramphenicol and relief by suA.


The gradient of polarity of z gene nonsense mutations in trp-lac fusion strains of Escherichia coli.


Starting point and direction of replication in P2 DNA.


Increased vascular permeability in the primary allograft response in the skin of the rat.


Biogenesis of microbial transport systems: evidnce for coupled incorporation of newly synthesized lipids and proteins into membrane.


Immunofluorescence investigation of human placentas.


Deformities of the renal tract associated with spina bifida in rat embryos.


Craniospinal dysraphia and segmental level of the axial skeleton.


Pathology of African histoplasmosis (H. duboisii)--light and electron microscopy.


The effects in the mouse of nerve crush and regneration on the innervation of skeletal muscles paralysed by Clostridium botulinum toxin.


The clinical aspects of renal transplantatio.


Tissue damage in transplantation imunity.


Amino-acid sequences of kangaroo myoglobin and haemoglobin and the date of marsupial-eutherian divergence.


Humerus of Dryopithecus from Saint Gaudens, France.


Simplification of the proton magnetic resonance spectrum of ribonuclease by difference spectroscopy.


Syphilis and Neanderthal man.

Syphilis and Neanderthal man.


Oral and subcutaneous administration of monosodium glutamate to infant rodents and dogs.


Therapeutic implications in Parkinsonism of m-tyramine formation from L-dopa in man.


DNA repair synthesis in mammalian cells exposed to a series of oncogenic and non-oncogenic derivatives of 4-nitroquinoline 1-oxide.


IgG subclass specificity of human monocyte receptor sites.


Effect of acetylcholine on glycogen formation and the activity of glycogen synthetase in isolated, perfused rat liver.


Effect of fluxes of sugars and mineral ions on the light microscopic structure of frog fast muscle fibres.


Inhibition of macrophage migration by a soluble factor from lymphocytes stimulated with PHA or ALS.


Control of bone growth in rats.

Control of bone growth in rats.


Impairment of antibody response and recovery in malarial rodents by antilymphocyte serum.


Isolation of Nocardia from soil by a modified paraffin bait method.


James Walton Groves (1906--1970).

James Walton Groves (1906--1970).


Xg blood groups of Thais.

Xg blood groups of Thais.


Fungus research.

Fungus research.


Transferrin.

Transferrin.


Kinetic evidence for incorrectly folded intermediate states in the refolding of denatured proteins.


Amino-acids, aliphatic and aromatic hydrocarbons in the Murchison Meteorite.


Configuration of amino-acids in carbonaceous chondrites and a Pre-Cambrian chert.


Effect of lithium on human aggression.

Effect of lithium on human aggression.


Effects of maternal preimmunization on the decidual cell reaction in mice.


Antiviral effect of constituent parts of the rifampicin molecule.


Tissue perfusion measured using the ratio of 81Rb to 81mKr incorporated in the tissue.


Acellular corneal transparency.

Acellular corneal transparency.


Removal and separation of flagella from cells of Clostridium chauvoei and preparation of a pure flagella suspension.


Cholesterol and hyperbaric oxygen in swimbladders of deep sea fishes.


Leukocytosis in the mouse induced by serial blood sampling from the tail.


Possible mechanism of egress of free cholesterol from the arterial wall.


Attenuation of stimulus sensitivity induced by scopolamine.


Conjugation defect in tyramine-sensitive migraine.


Where doctors draw the line.

Where doctors draw the line.


Brain drain from developing countries.

Brain drain from developing countries.


Water in biological systems.

Water in biological systems.


A prototype live oral cholera vaccine.

A prototype live oral cholera vaccine.


Different template specificities of phage T3 and T7 RNA polymerases.


Corneal sensitivity after grafting.

Corneal sensitivity after grafting.


George E. Palade.

George E. Palade.


Meal frequency--a possible factor in human pathology.


Incorporation of trans-fatty acids into tissue lipids.


Physiological role of lecithin-cholesterol acyltransferase.


Generalized infection of gnotobiotic piglets with E. coli of feline origin.


Thickening of arterial intima in rat cardiac allografts. A light and electron microscopic study.


Brachial plexus injury following median sternotomy incision.


Unusual complications during mitral valve replacement in the presence of calcification of the annulus.


Reconstruction of the mandible. Use of a homograft combined with autogenous bone and marrow.


Microsphere measurement of intrarenal circulation of the dog.


Effect of oxygen on growth of cultured myocardial cells.


List of literature on electron microscopy published in Japan.


The immune response to hapten-azo bovine serum albumin as detected by hemolytic plaque technique.


The survival of Streptococcus pyogenes on bacteriological swabs made from various fibres.


Aspects of colonisation of the mouse intestine by coliform bacteria.


Sterilisation of colicine preparations.

Sterilisation of colicine preparations.


A method of performing surface viable counts with Clostridium tetani.


Serial hemodynamic study of the transplanted heart and correlation with clinical rejection.


Drug-pacemaker interactions.

Drug-pacemaker interactions.


A simple modification of existing respirators to provide constant positive-pressure breathing.


Phenylethyl alcohol agar medium for isolation of anaerobic bacteria.


Alterations in lymphatic vessels in the course of chronic experimental lymphedema.


Surgical treatment of obstructed extracranial segments of the arteries supplying the brain.


A system of immunofluorescence in the study of tumour cells.


The effect of edeine on intracellular growth and mutation of bacteriophage T4B.


The course of multiple sclerosis in 73 cases with computer-designed curves.


Influences of television upon child psychiatry.


The use of imipramine in children's behavior disorders.


The children's psychopharmacology clinic: its role within a total program for children's psychiatric services.


[Evaluation of Tegretol in the treatment of behavior disorders in children. Methodology and results].


Obstetric features of thanatophoric dwarfism.


Potential efficiency of milk ring test for detection of brucellosis.


Identification of crystalline material in kidneys of rabbits.


Effects of certain anticorrosives in steam on clostridia.


Hair growth in skin grafts placed on hairless mice.


Experimental studies on the innervation of the diaphragm in cats.


The action of hormone metabolites: a new concept in endocrinology. A review on the metabolism and the activity of testosterone.


Bibliography. Middle ear.

Bibliography. Middle ear.


Dynamics of androgen metabolism in women with hirsutism.


Energy yields and growth of heterotrophs.


Metabolism and function of spermine and related polyamines.


Contributions toward the development of general microbiology (Ernst G. Pringsheim).


The genetic basis of antibody specificity.


Immunological enhancement as studied by cell culture techniques.


Bacterial cytochromes. II. Functional aspects.


The evolution of bacterial enzyme systems.


Reconstitution of viruses.

Reconstitution of viruses.


The regulation of glutamine synthesis in microorganisms.


Unique biochemical events in bacterial sporulation.


Microbiology of unconjugated pteridines.


The effect of thymine starvation on chromosomal structure of Escherichia coli JG-151.


Accelerated regeneration of transplanted hematopoietic stem cells in irradiated mice pretreated with cyclophosphamide.


Shirley A. Johnson.

Shirley A. Johnson.


Neuronal vascular relationships in the zona compacta of normal and parkinsonian substantia nigra.


The clinical physiology of calcium homeostasis, parathyroid hormone an, and calcitonin. I.


Parasitological review. Genetics of resistance to insecticides in houseflies and mosquitoes.


The primary structure of transfer ribonucleic acid.


Ultrastructural aspects of the pancreatic islets in carps of spontaneous diabetes mellitus.


Blood flow and gastrointestinal function.


A comparison of the enterococci index with coliform index as indication of faecal pollution of water.


Drug-induced leukopenia and aplastic anemia.


Bovine mastitis caused by Cryptococcus neoformans.


Effect of hydrocortisone on immune cytokinetics. I. Effects of different dose-schedules on 19S and 7S antibody forming cells in Jerne's plaque technique.


Ainhum (dactylolysis spontanea): a review of the literature and a report of two cases.


Avascular necrosis of bone after renal transplantation.


Cancer-progress and problems. Financial status of cancer research in the United States.


Effect of horseradish peroxidase on mice lung capillaries' permeability.


The ashing technique as a more precise method for the radioassay of 51Cr in feces.


The 99mTc labeling of erythrocytes.

The 99mTc labeling of erythrocytes.


Bone grafts of large cystic defects in the mandible.


Bone histogenesis and morphogenesis in implants of demineralized enamel and dentin.


Pyloromyotomy, a new technique in conjunction with esophagectomy or repair of hiatus hernia with vagotomy.


A simple modified method of collecting blood on filter paper for anti-HTV test in seroepidemiological studies on nasopharyngeal carcinoma.


Mitral insufficiency due to calcified myxoma. Treatment by resection and mitral annuloplasty.


A scandal ameliorated.

A scandal ameliorated.


Where to get your PhD.

Where to get your PhD.


Herbicide commission reports extensive damage.


Planning complex building systems.

Planning complex building systems.


Proposed mechanism for the biological assembly of collagen triple helix.


Was Virchow right about Neandertal?

Was Virchow right about Neandertal?


Effect of ethoxyquin on the longevity of C3H mice.


Stimulation by artificial lighting of calcium absorption in elderly human subjects.


Adverse influence of ozone on pulmonary bactericidal activity of murine lung.


Enhancement of residual antibody synthssis by irradiation of the spleen in pre-immunized rats.


Correlation between psychotropic potency of psychotomimetic methoxyamphetamines and their inhibition of 3H-normetanephrine uptake in rat cerebral cortex.


Growth potential of scrapie mouse brain in vitro.


Functional studies of reeenerated muscles from normal and dystrophic mice.


Autolytic enzymes in growth of bacteria.


Selective predation by newts on frog tadpoles treated with DDT.


R factor with a mutation in the tetracycline resistance marker.


Neuromythology?

Neuromythology?


High resolution nuclear magnetic resonance studies of hydrogen bonded protons of tRNA in water.


ADN programs accredited by the National League for Nursing--1971, Department of Associate Degree Programs.


Human blastocyst grown in vitro in ovulation cervical mucus.


Sleep paralysis in F. Scott Fitzgerald's The Beautiful and Damned.


Complications of the innominate osteotomy.


Haloperidol for acute psychiatric emergencies: a double-blind comparison with perphenazine in acute alcoholic psychosis.


Medicare--part B.

Medicare--part B.


Sarcomas of the stomach.

Sarcomas of the stomach.


Management of arterial injuries.

Management of arterial injuries.


Experience with first rib resection for thoracic outlet syndrome.


Fluorescent antibody staining of 1-2 mu-m sections of hyphae of Ophiobolus graminis Sacc. embedded in glycol methacrylate.


Adrenal cortical and medullary secretion and evaluation of drugs acting on the central nervous system.


Ribonucleic acids from the mitochondria of bleached Euglena gracilis Z. I. Isolation of mitochondria and extraction of nucleic acids.


The attachment of bacteria to solid surfaces.


Inactivation of Escherichia coli ribosomes by ultraviolet irradiation. 3. The activity of poly U-directed binding of phenylalanyl-tRNA.


Ribonucleic acids from the mitochondria of bleached Euglena gracilis Z. II. Characterization of highly polymeric ribonucleic acids.


The relationship of mono- and polynucleotide conformation to catalysis by polynucleotide phosphorylase.


The use of conformational changes to purify tryptophan tRNA from Escherichia coli.


Light-induced cross-linking of DNA in the presence of a furocoumarin (psoralen). Studies with phage lambda, Escherichia coli, and mouse leukemia cells.


Antibiotic YC 73 of Pseudomonas origin. 3. Synthesis of thioformin analogues.


High level transferable resistance to gentamicin.


Sporogony of the oocysts of Isospora felis Wenyon, 1923 from the cat.


The chools of Salerno--Medical and Artistic.


Dickens and medicosocial reform.

Dickens and medicosocial reform.


Doctors delinquent. II. The trials of two physicians charged with murder.


For mutualimprovment in the heang art. Philadelphia Medical Societies of the 18th century.


Reconstruction of the left anterior descending coronary arery. Proximal ein bypass graft and distal gas endarterectomy.


Mrs. Beeton, Medical adviser.

Mrs. Beeton, Medical adviser.


Literary style in American medical writing. A historica view.


Tobia Smollett. Doctor by design, writer by choice.


Doctor François Rabelais. Healer of body, anatomist of knowledge.


Kenneth Roberts (1885-1957).

Kenneth Roberts (1885-1957).


Sir Lindor Brown.

Sir Lindor Brown.


One-stage palate reconstruction with a free neo-vascularized jejunal graft.


Double pendulum flaps for whistling deformities in bilateral cleft lips.


Congenital arteriovenous anomalies of the head and neck.


The classic reprint: Graefe's first closure of a cleft palate.


The mysterious A. C. Valadier.

The mysterious A. C. Valadier.


New route for passage of forehead flap to inside of mouth.


Island flap innervated by radial nerve for restoration of sensation in an index stump. Case report.


Internal-external locus of control: a bibliography.


Comparison of four intelligence tests used with culturally disadvantaged children.


Fuller Albright, 1900-1969.

Fuller Albright, 1900-1969.


Arlie Ray Barnes, 1892-1970.

Arlie Ray Barnes, 1892-1970.


Stanhope Bayne-Jones, 1886-1970.

Stanhope Bayne-Jones, 1886-1970.


Thomas Francis, Jr., 1900-1969.

Thomas Francis, Jr., 1900-1969.


Francis Raymond Keating, Jr., 1911-1969.


James D. Heard, 1870-1967.

James D. Heard, 1870-1967.


John Bamber Hickam, 1914-1970.

John Bamber Hickam, 1914-1970.


William Watt Graham Maclachlan, 1885-1966.


Edwards Albert Park, 1877-1969.

Edwards Albert Park, 1877-1969.


Thomas Parran, 1892-1968.

Thomas Parran, 1892-1968.


Peyton Rous, 1879-1970.

Peyton Rous, 1879-1970.


Solomon Strouse, 1882-1966.

Solomon Strouse, 1882-1966.


Presentation of the Kober Medal for 1970 to Dickinson W. Richards.


Association of American Physicians. Membership list.


Inborn and acquired abnormalities of the complement system of man.


The effect of estriol succinated on the rejection of kidney transplants.


Prolongation of skin allograft survival in C57BL and C3H mice by L-asparaginase treatment.


Use of procarbazine hydrochloride (natulan) and procarbazine hydrochloride and azathioprine in canine renal allografts.


Successful use of chondroitin-4-sulfate in prolonging survival of skin allografts of adult mice.


Prolongation of rabbit skin allograft survival following treatment of the skin with electrophoresis prior to transplantation.


Effects of adrenalectomy and antigenic stimulation on spleen weight in mice.


Functioning heart homografts in hamsters.


Histocompatibility genes of mice. X. Additional non-H-2 typing.


Prolonged survival of renal allografts in rats mismatched at the major histocompatibility locus.


Evaluation of preservation of cadaver kidneys.


Significance of a negative cross match in recipients with a wide spectrum of lymphocytotoxic antibodies.


Kinetic studies of macrophages. Homograft response.


Further developments of the rosette inhibition test for the testing of antihuman lymphocyte serum.


Membership list.

Membership list.


Master impressions for fixed restorations.


Rx for lab work--why?

Rx for lab work--why?


The advantages of wet high speed cutting.


Health legislation.

Health legislation.


Alveolar ridge deficiency correction with osseous transplant and vestibuloplasty skin graft. Report of case.


Maryland Dental Assistants certified by radiology course.


Dentists licensed as result of Maryland and North East Regional Board Examinations--October 20-21, 1970.


Roster of Members--1971--Nebraska Dental Association.


Amino acid pool of Escherichia coli during the different phases of growth.


Directories of education programs and services. A. Educators of the deaf. B. Schools and classes (program, pupil and staff data). C. Post-secondary programs. D. University programs training teachers. E. Federal Offices providing services for education.


The effect of branched chain amino acids on the synthesis of isoleucine from threonine and pyruvate in crude extracts from Escherichia coli.


Timely topics in clinical chemistry: a review, 1969.


Nucleotide distribution of Escherichia coli 16S ribosomal ribonucleic acid.


Quantitative measurement of isoprenoid nuceosides in transfer ribonucleic acid.


Transformation of reticulum cell sarcoma to acute leukemia.


Chronic myelogenous leukemia in Hodgkin's disease: immunofluorescence of cells.


Comparison of busulfan and cyclophosphamide in the treatment of chronic myelocytic leukemia.


Malignant potential of a cell line isolated from the peripheral blood in infectious mononucleosis.


A development study of the subject matter choices in the free drawing of trainable mentally retarded subjects.


Fine structure of the synaptonemal complex. Regular and stereo electron microscopy of deoxyribonuclease-treated whole mount preparations.


Separation of mast cells by centrifugal elutriation.


Synchronization of Chlamydomonas division with intermittent hypothermia.


Biochemical aspects of the attachment of a pig-kidney monolayer cell line to glass surfaces.


Whole mount electron microscopy of human meiotic chromosomes.


L-asparaginase A from Escherichia coli. The molecular weight of the subunit and the demonstration of an intramolecular disulfide bridge.


Cytosine nucleotides in liver.

Cytosine nucleotides in liver.


An examination of frustration-aggression relations in boys during middle childhood.


Creativity as related to field independence and mobility.


Immunological identification and quantifications of guinea pig heterophil and eosinophil peroxidases.


Pediatrics in South Carolina.

Pediatrics in South Carolina.


Synthesis of antibody by lymphocytes restimulated in vitro with antigen.


Role of the galactose binding protein in chemotaxis of Escherichia coli toward galactose.


Ribosomes, G-factor and siomycin.

Ribosomes, G-factor and siomycin.


Members of the experimental psychology society.


Induction of closely linked multiple mutations by nitrosoguanidine.


Semidwarf rice and wheat in global food needs.


The cytology, distribution and function of the neoplastic cells in leukaemic reticuloendotheliosis.


Variations in rabbit antimouse lymphocyte sera caused by lymphocytes of differing origins in the immunizing inocula.


Nonpreferential uptake of antithymus globulin in canine renal allografts.


The immunosuppressive effect of antilymphocyte sera produced by sensitization with different lymphoid tissues.


Susceptibility of parental strain and F1 hybrid rats to runt disease.


A model for inducing tolerance to RS rat tumor in mice.


Plaques of cutaneous necrosis in skin-homografted mice. A method of prevention.


Mechanisms of tumor-specific enhancement versus resistance toward a methylcholanthrene-induced murine sarcoma.


The influence of the hair follicle phase on the survival time of skin allografts in the mouse.


Inhibition of rosette formation by spleen cells from grafted mice treated with anti-lymphocyte serum.


Immunosuppression across weak histocompatability barriers. I. 6-mercaptopurine in inbred rabbits: graft survival and toxicity.


The effect of x-rays on the salt stimulation of DNA-dependent RNA synthesis in vitro.


Application of gel adsorption to characterize strains of the family Enterobacteriaceae.


Effect of factor C on glucose repression of induced beta-galactosidase synthesis.


Specific oral prevention of infantile gastro-enteritis. I. Experiments in mice.


Immunohistochemical evidence for the transport of neurophysin in neurosecretory neurons of the dog.


Principles of skin grafting in burns.

Principles of skin grafting in burns.


Laminar airflow protection in bone marrow transplantation. A laminar airflow room was used to provide a low-pathogen environment for a child with lymphopenic immune deficiency transplanted with paternal bone marrow. Comparison of flora from the patient, personnel, and the environment indicated that no colonization with exogenous organisms occurred in the patient during the 45-day period of study. The number of organisms recovered from the laminar airflow room was exceedingly small. Conventional hospital isolation rooms contained more bacteria and fungi than the laminar airflow room, even when strict aseptic procedures were followed in the former. Patients with lymphopenic immune deficiency and agranulocytosis admitted to conventional isolation rooms were colonized with exogenous organisms within 1 week. Each developed infection with these strains, and one patient died. Laminar airflow isolation seems at present the best means to prevent exogenous infection during hospitalization of patients with lymphopenic and other severe immune-deficiency diseases and may be essential when bone marrow transplantation is performed to treat their immunological defect.


Comparison of the rates of motility of Salmonella with those of other enteric bacteria. Comparison of the rates of motility through a semisolid medium of 16 common Salmonella sp., 14 Escherichia coli serotypes, 4 Arizona strains, 2 Escherichia freundii (Citrobacter) isolates, 2 Proteus sp., and 2 Pseudomonas sp. revealed the following. (i) Very closely related bacteria could demonstrate markedly different rates of progression. (ii) All of the salmonellae tested advanced faster than the Proteus and Pseudomonas test cultures but some Salmonella sp., notably S. choleraesuis and S. typhi, progressed relatively slowly compared to many other test cultures. (iii) The mean rate of motility for the fastest 14 Salmonella sp. (1.49 cm/hr) was not statistically greater than the mean value for the 14 E. coli serotypes (1.31 cm/hr) at the 1% level of significance. Selective motility procedures may not be a reliable means of isolating all Salmonella sp. from materials contaminated with other bacteria.


Use of a fiber optic probe for spectral measurements and the continuous recording of the turbidity of growing microbial cultures. This paper describes the properties and use of a fiber optic probe as an attachment to a spectrophotometer and its use for measurements in solutions and turbid suspensions. Measurements of a standard were identical when a spectrophotometer equipped with the probe was used or when a spectrophotometer was used in a conventional manner. The probe was calibrated for turbidimetric measurements with microorganisms by relating the apparent absorbancy measured on the spectrophotometer to the dry weight of each species of organism. Continuous measurements were made of the turbidity of growing cultures of Escherichia coli, Streptococcus mutans, and Saccharomyces cerevisiae. Transient changes in cell mass were observed in some cultures during continuous monitoring of growth. The data were recovered in a manner which allowed direct computer processing.


Antigenic relationships among the proteolytic and nonproteolytic strains of Clostridium botulinum. Relationships of the somatic antigens among Clostridium botulinum strains have been investigated by tube agglutination and agglutinin absorption tests. Results revealed a relationship by which strains of C. botulinum are grouped by their proteolytic capacity rather than by the type of specific toxin produced. Thus, C. botulinum type E and its nontoxigenic variants, which are nonproteolytic, share common somatic antigens with the nonproteolytic strains of types B and F. Absorption of antiserum of a strain of any one type with antigen of any of the others removes the antibody to all three types. In the same manner, C. botulinum type A shares somatic antigens with the proteolytic strains of types B and F, and absorption of any one antiserum with an antigen of either of the other two types removes the antibody to all three types. Partial cross-agglutination of C. sporogenes, C. tetani, and C. histolyticum with the somatic antisera of the proteolytic group was also observed.


Idiopathic ulcer of small bowel with pseudolymphomatous reaction. A clinicopathological study of six cases.


Bacteriology of manganese nodules. V. Effect of hydrostatic pressure on bacterial oxidation of MnII and reduction of MnO2. It was experimentally demonstrated that two strains of Arthrobacter 37, one growing at 25 C and the other at 5 C, could catalyze Mn(II) oxidation at hydrostatic pressures well in excess of the pressure encountered by the parent culture in its original habitat in the ocean (80 atm). The strain grown at 5 C showed an increase in temperature optimum for manganese oxidation with increase in pressure. It was like-wise experimentally shown that induced Bacillus 29 without added ferricyanide and uninduced Bacillus 29 with added ferricyanide could catalyze MnO(2) reduction at hydrostatic pressures in excess of the pressure encountered by this organism in its original habitat (187 atm). The uninduced Bacillus 29, in the presence of ferricyanide, was active over a wider range of pressures (1 to 1,000 atm) than the induced Bacillus 29 in the absence of ferricyanide (1 to 467 atm). At corresponding pressures, the uninduced culture was also considerably more active than the induced culture. Special techniques were developed for measuring Mn(II)-oxidizing and MnO(2)-reducing activity under pressure.


Participation of liver fractions and of intestinal bacteria in the metabolism of N-hydroxy-N-2-fluorenylacetamide in the rat.


Lipolytic activity of structurally related agonists in rat epididymal fat tissue in vitro.


Transmission of toxoplasmosis by leukocyte transfusion.


Dr. Henry M. Stratton--70 years.

Dr. Henry M. Stratton--70 years.


Origin of repopulating cells in a mechanically depleted medullary cavity as determined by studies with marrow transplants.


The unstable haemoglobins.

The unstable haemoglobins.


Fluorescent antibody techniques in the identification of the gram-negative nonsporeforming anaerobes.


Multiple tests for the laboratory diagnosis of gonorrhea.


Ileal microflora in surgical patients.

Ileal microflora in surgical patients.


Use of fecal coliform bacteria in evaluating microbial contamination in pediatric wards.


The effects of hydration and bladder incubation time on urine colony counts.


The effect of Hypaque upon urine cultures.


Fluorescence microscopy of urinary sediment.


A urological evaluation of cephalexin.

A urological evaluation of cephalexin.


Bacterial flora of urine from ileal conduit.


Chronic pyelonephritis as a cause of renal failure in dialysis candidates. Analysis of 173 patients.


Vi agglutinins in Salmonella infections.


Eradication of gametocytes to prevent introduction of falciparum malaria.


"Reversion" of DNA polymerase-deficient Escherichia coli.


Escherichia coli strains with modified RNase II activity: isolation and properties.


Hospital patient statistics. Problems and prospects.


Toxoplasmosis isosporosis.

Toxoplasmosis isosporosis.


Depressions in childhood and adolescence. 1.


Edwin Allen Locke, M.D.

Edwin Allen Locke, M.D.


Augmentation of sodium and potassium excretion, glomerular filtration rate and renal plasma flow by levodopa.


Objectives of oxygen therapy.

Objectives of oxygen therapy.


Prothrombin time standardization.

Prothrombin time standardization.


In memoriam, Carroll F. Shukers, M.D., 1905-1970.


Eli M. Bower, ed.d. President, American Orthopsychiatric Association, 1971-1972.


Community child psychiatry: evolution and direction.


Morphology of bacteriophages used for typing Shigella sonnei.


Mediators and nonsteroid antiinflammatory drugs in immunologically specific and nonspecific inflammatory processes.


The nature of the lethal factor of Shigella flexneri.


The lethal action of ehtyl methanesulfonate, nitrogen mustard and myleran on the T7 coliphage.


T7 coliphage inactivation by nitrous acid.


Sedimentation analysis of DNA degradation products resulting from the action of colicin E2 on Escherichia coli.


The biosynthesis of inosinic acid in transfer RNA.


A protein factor stimulating binding and translating of polyuridylic acid by Escherichia coli ribosomes.


Raman spectral studies of nucleic acids. 3. Laser-excited spectra of ribosomal RNA.


Ribosomal protein pool of Escherichia coli.


Immunoglobulin G subclasses of antinuclear antibodies and renal deposits. Comparison of systemic lupus erythematosus, drug-induced lupus and rheumatoid arthritis.


The synthesis of rapidly labelled RNA in a beta-galactosidase constitutive mutant of Escherichia coli.


The release of DNA into serum and synovial fluid.


Changes in hybridizable nuclear RNA during differentiation of mammary cells.


A purification procedure for small amounts of radioactive Escherichia coli RNA polymerase.


Messenger ribonucleoprotein complexes released from rat liver nuclei by ATP. I. Characterization of the RNA moiety of messenger ribonucleoprotein complexes.


Reversal of the inhibitory action of chloramphenicol on the ribosomal peptidyl transfer reaction by erythromycin.


Surgical treatment of coronary heart disease.


Medical fore-edge decorations and paintings.


Reye's syndrome. Possible causes and pathogenetic pathways.


The problem of compatibility in hematology with particular reference to bone marrow transplantation.


Separation of unbound iodide in 125-I-labeled antipyrine.


Polar mutations in the left arm of bacteriophage lambda i434. By studying complementation between frameshift and nonsense mutants located in the structural genes for the head of bacteriophage lambdai434, we found mutations in gene B which are polar on genes C and D and one mutation in gene E which is polar on gene F.


Photodynamic action of proflavine on coliphage T3. I. Kinetics of inactivation. The photodynamic inactivation of coliphage T3 was studied over a wide range of concentrations of the dye proflavine. With 2 x 10(7) phage/ml, two modes of inactivation were observed. Between 0.25 and 12 to 13 mug/ml, inactivation was biphasic. There was an initial first-order inactivation (Rx1) which became temporally associated with an apparently multiorder process (Rx2) at higher light doses. Dye concentrations above 12 to 13 mug/ml showed only two-target inactivation curves (Rx3), except at high dye concentrations where processes kinetically identical to Rx1 and Rx2 reappeared. Rx2 showed a normal rectangular hyperbolic saturation curve but Rx1 and Rx3 appeared to saturate prematurely. The saturation behavior of Rx1 and Rx2 was independent of phage concentration, but Rx3 was lost at phage titers above 2 x 10(7)/ml. No dark inactivation was seen with Rx1 and Rx2 subsequent to a period of illumination. With Rx3, an exponential dark inactivation was seen for at least 1 hr after a period of illumination. The dye-phage system equilibrated immediately, at any temperature, at proflavine concentrations where Rx1 and Rx2 occurred. With Rx3, prolonged equilibration times were necessary. Moreover, there was a temperature effect. The rate of inactivation at equilibrium was temperature-dependent, whereas the initial rate at which equilibrium was approached was essentially temperature-independent.


Photodynamic action of proflavine on coliphage T3. II. Protection by L-cysteine. Three kinetically different reactions (Rx1, Rx2, and Rx3) have been distinguished in the photoinactivation of phage T3 in the presence of the dye proflavine. The response of these reactions to the presence of the radical trap l-cysteine has been examined. At dye concentrations equal to or less than 2.2 mug/ml, Rx1 was composed of at least two parallel first-order reactions, one cysteine-insensitive (Rx1A) and one cysteine-inhibited (Rx1B). Rx2 was completely cysteine-insensitive (Rx2A). The cysteine sensitivity of these reactions changed abruptly at dye concentrations above 2.2 mug/ml. Rx1A and Rx1B now operated in tandem, rather than simultaneously, with Rx1B being confined to the first 1 min at most. Rx2, on the other hand, was completely cysteine-inhibited (Rx2B). Rx3 was inhibited roughly 75 to 80% by saturating concentrations of cysteine regardless of the time of addition of cysteine. The dark inactivation associated with Rx3 was inhibited roughly 85% whether the radical trap was added during the light or dark regimes. Changes of initial phage titer did not alter the cysteine sensitivity of a reaction.


Photodynamic action of proflavine on coliphage T3. 3. Damages to the deoxyribonucleic acid associated with Rxl and Rx2. Ultracentrifugational studies with deoxyribonucleic acid (DNA) extracted from phage exposed to light in the presence of either 0.25 or 8.5 mug of proflavine per ml reveal that the lethal damage of Rx1 renders DNA alkali-labile, with lethality resulting from damage that occurs singly on either strand or simultaneously on both strands. Apparently nonlethal damages temporally associated with Rx1 consist of (i) heat and alkali-labile cross-links (which produce undenatured DNA that migrates at 45S) and (ii) heat-labile bonds. The formation of 45S material is a linear function of light dose, and the production of this material ceases when Rx2 appears at the higher dye concentration. No tendency to plateau is seen at 0.25 mug of dye per ml. The nature of the lethal damage of Rx2 could not be determined. Damages that were temporally associated with Rx2 at 8.5 mug of dye per ml were heat-labile, alkali-stable cross-links (undenatured DNA, 38 to 41S; alkali-denatured-reneutralized DNA, 85 to 89S) and some double-stranded breaks. No such changes were seen at 0.25 mug of dye per ml.


Human research model for the production and prevention of gingivitis.


Effect of elevated temperature on extended enzyme synthesis induced by bacteriophage T4 amber mutants unable to synthesize deoxyribonucleic acid. The extended synthesis of early enzymes by the deoxyribonucleic acid-negative amber mutants of bacteriophage T4 after infection of the nonpermissive host Escherichia coli B was prevented by incubating the infected cells at 44 C. This effect did not occur if the incubation temperature was 43 C or less or if the cells were grown and infected in broth rather than minimal medium (C medium). Once early enzyme synthesis had ceased at 44 C, lowering the incubation temperature to 37 C did not occasion resumption of synthesis. Experiments with chloramphenicol at 44 C indicated that increased degradation of early enzymes is an unlikely explanation for the effect. Examination of pulse-labeled ribonucleic acid and polysomes made at 37 and 44 C in infected cells revealed some differences, but at present there is no obvious way in which these differences may be related to the effect on enzyme formation. There was no discernible difference between the ribosomal ribonucleic acid and ribosomes at the two temperatures, nor was there a difference in the cell-free amino acid-incorporating systems isolated from cells infected at the two temperatures as judged by polyuridylic stimulation of phenylalanine incorporation. Incubation of cells infected with T4amN82 at 44 C with protein synthesis blocked by 5-methyltryptophan for 15 min did not prevent the typical pattern of enzyme synthesis at 44 C when the block was reversed by excess l-tryptophan. The relation of this and other observations relative to the effect at 44 C on the synthesis of early enzymes is discussed.


Recent progress in collagen research.

Recent progress in collagen research.


General discussion. Usefulness and limitations of model systems.


Repair of alkylated bacteriophage T4 deoxyribonucleic acid by a mechanism involving polynucleotide ligase. Methyl methanesulfate-induced lesions in bacteriophage T4 are repaired primarily by a mechanism involving polynucleotide ligase. Apparently, other recombinational and ultraviolet repair functions aren't involved.


Effect of myxin on deoxyribonucleic acid synthesis in Escherichia coli infected with T4 bacteriophage. Exposure of Escherichia coli cells to myxin results in the almost complete inhibition of new deoxyribonucleic acid (DNA) synthesis, extensive degradation of pre-existing intracellular DNA, and a rapid loss of viability in these cells (9). After exposure to myxin for 30 min (<1% survivors and >25% degradation of DNA), infection of these cells by T4 bacteriophage results in the renewal of DNA synthesis at a rate essentially equal to that found in T4-infected cells in the absence of myxin. This DNA was characterized as T4 DNA by hybridization and by hydroxyapatite chromatography. These results suggest that the primary site of action of myxin does not involve the biochemical pathways involved in either the energy metabolism or the biosynthesis of DNA precursors in the uninfected host cell. The yield of infectious T4 particles was reduced when myxin was present during multiplication. This effect may be partly accounted for by the finding that a significant fraction of the T4 DNA synthesized in the presence of myxin is apparently not properly enclosed by the bacteriophage protein coat since it is shown to be degraded by exogenous nuclease.


Stereotactic surgery in temporal lobe epilepsy.


Sedative neurosurgery.

Sedative neurosurgery.


Two machine stereotaxy.

Two machine stereotaxy.


Stereotaxic surgery in cerebral palsy--a study of 57 cases.


The size of the third ventricle in British and Indian subjects.


Quantitative microdetermination of total cysteine plus cystine in proteins.


Comparison of the effects of some iodine compounds on the survival of Escherichia coli B-r irradiated with X rays and ultra-violet light.


Iron transport in Escherichia coli and its relation to the repression of 2,3-dihydroxy-N-benzoyl-L-serine synthetase.


Studies with tRNA adenylyl(cytidylyl)transferase from Escherichia coli B. I. Purification and kinetic properties.


Studies with tRNA adenylyl(cytidylyl)transferase from Escherichia coli B. II. Regulation of AMP and CMP incorporation into tRNApCpC and tRNApC.


Air disinfection in corridors by upper air irradiation with ultraviolet.


A binding protein for L-glutamine and its relation to active transport in E. coli.


Antibiotic sensitivity patterns and R factors in domestic and wild animals.


The gold of the north. Amber trading as an occupation.


Effects of sulfur oxides and particulates on health.


The influence of interactive stimuli on rhythmical masticatory movements in rabbits.


Morphology of the interface between adhesive resins and treated human enamel fissures as seen by scanning electron microscopy.


Eta nova et vetera.

Eta nova et vetera.


The arms of the colleges of surgeons. II. England, Ireland, physicians and surgeons of glasgow.


The clinical electroretinogram after 25 years.


The synthesis of a protein with acyl carrier protein activity.


Phasing out Medicare: changing definitions of skilled nursing care and custodial care.


Protein structure and properties.

Protein structure and properties.


Neurogenesis in the optic tectum of larval Rana pipiens following unilateral enucleation.


Separation of normal and immune lymphoid cells by antigen-coated coated columns. Antigen-binding characteristics of membrane antibodies as analyzed by hapten-protein antigens. Specific fractionation of immunologically competent cells derived from normal or immune animals was achieved by filtration through antigen-coated bead columns. This selective retention of the relevant reactive cells could be shown to produce a cell population, which after passage through the column would behave like a suspension rendered immunologically tolerant by "classical" means. The immunologically specific elimination of potential antibody-forming capacity of the filtered cells could be shown to affect the IgM and the IgG system to a similar extent. Analysis of the binding characteristics of the membrane antibodies responsible for this selective retention indicate striking similarities to those of the humoral antibodies produced against the antigen. Thus, the surface receptor could distinguish isolated haptenic groups on a "foreign" carrier background and the receptor of the hapten-reactive cells in the present system (high-rate antibody-forming cells against NIP) failed to combine with carrier specific determinants in analogy with the binding behavior of the serum anti-hapten antibodies. The binding of anti-hapten reactive cells to bead-attached haptens could be specifically inhibited by the presence of free hapten in the columnar fluid during cellular filtration. The results strongly suggest that the potential humoral antibody-forming cell has preformed receptors on its outer surface with binding characteristics, indicating similarity, if not identity, to those of the eventual product of the cell, the humoral antibody.


Antibody-mediated suppression of the immune response in vitro. II. A new approach to the phenomenon of immunological tolerance. Immunological tolerance to H antigens of Salmonella adelaide may be induced in vitro by the exposure of mouse spleen cells for 6 hr to an immunogenic dose of polymerized flagellin in the presence of low concentrations of specific antibody. Such antibody-mediated tolerance requires an optimal antigen: antibody ratio for its induction. A shift in this ratio in favor of the antibody concentration results in failure of tolerance induction and leads to immune suppression commonly known as antibody-mediated feedback inhibition which is not analogous to immunological tolerance. Fragment A of flagellin fails to induce immunological tolerance in vitro. Tolerance to polymerized flagellin may however be induced in vitro, provided the spleen cells are exposed to fragment A in the presence of specific antibody for 6 hr. The results are discussed in the light of current theories of the mechanism of tolerance induction.


Studies on the regulation of avidity at the level of the single antibody-forming cell. The effect of antigen dose and time after immunization. The hemolytic plaque formation of cells producing antibody against heterologous albumins was tested for sensitivity to specific inhibition by free antigen. The inhibition characteristics of plaques in this system were found to be a measure for the avidity of the antibody produced by the plaque-forming cells (PFC:s). High avidity-producing PFC:s were more sensitive to inhibition than low avidity PFC:s. Immunization with a high dose of antigen induced PFC:s that produced antibody with a lower avidity as compared to PFC:s from animals immunized with a low dose. The avidity was increased with time. Determinations of avidity at the serum level were also made, and the results were in agreement with the findings at the cellular level. The present method made it possible to demonstrate differences in avidity of antibody at the level of the single antibody-forming cell. It may also constitute a useful tool for the analysis of the cellular events leading to the production of antibodies with varying affinities during the immune response.


The anaerobic bacterial flora of the mouse cecum. Refinements of the anaerobic technique such as the utilization of prereduced media and the removal of traces of oxygen from the gases used by passage through a hot copper oven resulted in quantitative and qualitative improvements in the recovery of anaerobic bacteria from the cecum of SPF mice. The commonest morphologic types of bacteria in the mouse cecum were tapered rods. These characteristically gave "speckled" colonies on agar plates. Those identified were species of the genera Fusobacterium, Eubacterium, and Clostridium. Several of the morphologic types seen with phase microscopy still could not be cultivated. The implications of these findings for the study of the intestinal flora are discussed.


Antigen-specific synergism in the immune response of irradiated mice given marrow cells and peritoneal cavity cells or extracts. A synergistic immune response to foreign erythrocytes may be induced in heavily irradiated mice injected with a mixture of isologous cells obtained from marrow and from the peritoneal cavity. Under appropriate conditions, homologous or heterologous peritoneal cavity cells, heat-killed cells, or cellfree extracts made from such cells are also effective. The activity of the peritoneal cavity cells or extracts is antigen-specific, in the sense that cells or extracts obtained from animals previously immunized with the test antigen produce much stronger synergistic effects than do cells from animals immunized with some other antigen; however, the peritoneal cavity cells or extracts are not immunogenic when tested in primed animals. The marrow cells, demonstrated to contain precursors of the antibody-forming cells produced during this synergistic immune response, also show a form of antigen-specificity.


Some physical and radiobiological properties of immunologically reactive mouse spleen cells. Three classes of immunologically reactive cells, differing only slightly in size from each other, are required for the production of hemolysin-forming cells in culture. The three classes of cells can be detected in the normal mouse spleen by the combined use of rosette formation, velocity sedimentation, and irradiation. One class of cells (peak sedimentation velocity, 3.2 mm per hr) forms rosettes. The capacity of these cells to participate in immune responses to foreign erythrocytes is inhibited by relatively low doses of irradiation. These cells may be the immediate precursors of hemolysin-forming cells. A second class of cells (peak sedimentation velocity, 3.6 mm per hr) facilitates the production of hemolysin-forming cells by small numbers of normal spleen cells. Their facilitative activity is resistant to a relatively large dose of radiation. They do not form rosettes. The requirement of a third class of cells was deduced from the results of mixing experiments. Neither rosette-forming cells nor spleen cells largely depleted of rosette-forming cells could give rise to hemolysin-forming cells when cultured either alone or in the presence of large numbers of heavily irradiated cells. However, when rosette-forming cells, cells depleted of rosette-forming cells, and heavily irradiated cells were mixed together, hemolysin-forming cells were produced. The peak responses were found in fractions sedimenting at 4 mm per hr. Thus, it is suggested that these fractions contain a third class of cells. This class of cells does not form rosettes, but its function is inhibited by relatively low doses of radiation.


On the mechanism of type I Alzheimer abnormality in the nuclei of astrocytes. An essay in quantitative histology.


Multiple sclerosis: progress in epidemiologic and experimental research. A review.


Identification of filamentous microorganisms in activated sludge.


Total coliform: fecal coliform ratio for evaluation of raw water bacterial quality.


Neural-tube defects: an unrecognized epidemic.


Fingertip injuries.

Fingertip injuries.


Acute and chronic studies using a new oral hypoglycemic agent, glyburide.


Interaction of RNA polymerase mutations in haploid and merodiploid cells of Escherichia coli K-12.


Some aspects of nutrition in Philophthalmus burrili (Trematoda: Digenea).


Structural differences in the egg envelopes of Haemonchus contortus and Trichostrongylus colubriformis (Nematoda: Trichostrongylidae).


Cross immunity between mycobacterial and Pseudomonas pseudomallei infections in mice.


Medicine's lesson from history.

Medicine's lesson from history.


Capuride for sleep in rehabilitation patients: a controlled trial.


Relative efficacy of intraperitoneal application of haematopoietic cells to irradiated mice.


Levator resection for minimal ptosis, with indications and reappraisal.


Congenital and surgical anophthalmia.

Congenital and surgical anophthalmia.


Therapeutic contact lenses.

Therapeutic contact lenses.


Ectropion and entropion.

Ectropion and entropion.


The palpebral spring for paralysis of the upper eyelid.


Tumors involving the orbital region.

Tumors involving the orbital region.


Baggy lids.

Baggy lids.


Trauma and burns.

Trauma and burns.


Blepharoptosis: clinical evaluation and therapeutic rationale.


Embedded figures performance of schizophrenics as a function of chronicity, premorbid adjustment, diagnosis, and medication.


Social schemata in schizophrenic males.

Social schemata in schizophrenic males.


Cellular differentiation of the immune system of mice. VI. Strain differences in class differentiation and other properties of marrow cells. Marrow cells and 5 x 10(7) thymocytes of unprimed (C57BL/6 x DBA/2)F(1), (C57BL/10 x WB)F(1) and (C3H x C57BL)F(1) donor mice were mixed in vitro and transplanted into X-irradiated syngeneic hosts. Upon injection of sheep erythrocytes, splenic plaque-forming cells (PFC) secreting IgM (direct PFC or IgG (indirect PFC) hemolytic antibody were enumerated at the time of peak responses. By grading the numbers of marrow cells, inocula were found that contained few immunocompetent cells reaching the recipient spleens, interacting with thymocytes or other accessory cells (or both), and generating PFC. The frequency of responses in BDF(1) mice conformed to Poisson statistics, indicating that immunocompetent marrow cells participated in a single-hit interaction limiting PFC responses. The marrow cells assayed were not restricted for the antibody class (IgM versus IgG) to be secreted by mature PFC. Unrestricted marrow cells could have been either the precursors of PFC or accessory cells. Different results were obtained in BWF(1) and C3BF(1) mice. The frequency of responses in relation to the number of marrow cells grafted did not follow Poisson statistics, and the limiting cells were restricted for antibody class. Presumably, immunocompetent cells of these strains were more heterogeneous than those of BDF(1) mice and participated in a multiplicity of cell-to-cell interactions. The strain differences reflected inherent properties of marrow cells and not influences of the environment in which PFC were produced. The results confirmed for bone marrow the heterogeneity of immunocompetent cells reported by others for spleen, and suggested that genetic factors such as "immune response" genes regulate cellular differentiation also for functions other than those related to antibody specificity.


The Toxoplasma gondii oocyst from cat feces. Coccidian oocysts resembling those of Isospora bigemina were excreted by cats fed Toxoplasma. In order to identify these oocysts with Toxoplasma infectivity a number of critical comparisons were made. The appearance of oocysts and Toxoplasma infectivity was simultaneous in the feces of 23 of 24 adult cats, 3-5 days after feeding of Toxoplasma cysts; in the feces of 4 out of 9 cats, 7-10 days after feeding of trophozoites; and in 8 out of 17 cats, 20-24 days after feeding of cat feces containing oocysts. Oocysts and infectivity were present in similar numbers, and they disappeared simultaneously from the feces of cats. Oocysts and infectivity were also observed simultaneously in the feces of 9 kittens, 1-2 days old, fed Toxoplasma cysts. Oocysts could not be separated from infectivity by filtration, by continuous particle electrophoresis, or by density gradient centrifugation. Excystation of oocysts was followed by an increase in titer of Toxoplasma infectivity. Unsporulated oocysts in fresh cat feces were noninfectious to mice, but oocyst sporulation was associated quantitatively with the development of infectivity at different temperatures and conditions of oxygenation. Maximum oocyst sporulation at 48 hr correlated with the development of maximum Toxoplasma infectivity. 1 and 2% sulfuric acid, and 2.5% potassium dichromate were found to be the best preservatives for sporulation of oocysts and for the development of Toxoplasma infectivity. Low sporulation rates in 0.1% formalin, 20% ethanol, and in water were associated with low infectivity in these reagents. Neither Toxoplasma infectivity nor oocysts developed in 0.3% formalin, 1% ammonium hydroxide, or 1% iodine in 20% ethanol. Oocysts, sporocysts, and sporozoites were stained specifically with Toxoplasma antibody in the indirect fluorescent antibody test. Typical coccidian stages, schizonts, and male and female gametocytes were found in the epithelium of the small intestine of kittens fed Toxoplasma cysts. The classification of T. gondii is discussed in relation to that of other isosporan coccidia of cats and dogs. The term "Toxoplasma oocyst" is introduced and Toxoplasma is classified in the family Toxoplasmidae of the suborder Eimeriina. The species Isospora bigemina is restricted to dogs, and I. cati to cats. I. felis and so-called I. rivolta from cats were noninfectious to dogs, and did not confer immunity to subsequent infection with I. canis and I. rivolta from dogs.


Studies of the relationship between the production of bacteriocines by group A streptococci and acute glomerulonephritis. The previously reported relationship between nephritogenicity and bacteriocine production among Group A streptococci has been examined. Using techniques comparable to the earlier study, the present study did not reveal any such relationship. Among 73 strains tested, 53% produced bacteriocines; no significant difference was noted between bacteriocines from strains isolated from cases of nephritis and those from a group of strains recovered from patients with other conditions. In addition, no correlation between type and bacteriocine production was observed.


Implantation, transplantation, and epithelial-mesenchymal relationships in the rat uterus. Free tail skin grafts or suspensions of viable epidermal cells have been placed in the atraumatized uterus of isologous rat hosts and allowed to "implant" of their own accord to study the possible uniqueness of this site for other than nature's transplants, i.e. conceptuses, and its response to unnatural grafts. Despite the presence of an intact endometrial epithelium, free skin grafts heal-in rapidly, provided that a state of estrogen excess is established at the time of transplantation. In the absence of estrogen most of the grafts failed to implant. Once established, the grafts survive indefinitely without further estrogen. However, if at any stage a state of continual estrus is established, skin epidermis migrates centrifugally from the graft perimeter invasively replacing the native uterine epithelium. The results of an analysis of the modus operandi of this estrogen-facilitated epidermal migration in utero sustain the view that the hormone acts upon the uterine stroma rather than upon the epidermal cells. When grafts of lingual mucosa or vaginal "skin" were placed in the uteri of rats maintained in chronic estrus, migration of epidermis took place even more vigorously than from tail skin. These epithelia conserved their distinctive histologic specificities indefinitely when growing as heterotypic recombinants on the alien mesenchymal stroma of the uterus. Monodisperse suspensions of epidermal cells appear to "implant" and establish small epidermal plaques in the uterus only at sites predestined to accept conceptuses. That the endocrinologic parameters for the establishment of skin grafts in the uterus are similar to those for blastocysts is suggested by the finding that both kinds of graft can become established in the same uterine horn in the absence of exogenous hormones.


Intrarenal complement fixation by cytotxic antibodies. The reactivity of histocompatibility antigens with serum antibodies and complement was studied in a short term model of allotransplantation. Lewis and DA rat kidneys were perfused with cytotoxic antisera and tissue sections analyzed for adsorption of fluorescent antibodies against gamma-globulin and complement (beta(1C) protein). Both, the presumed cytotoxic antibody and C were localized at similar sites in the endothelial lining and in the walls of the arterial vessels and of the larger veins. The pattern of immune globulin deposition was distinct from that observed in glomerulonephritis. It may thus become helpful in the differential diagnosis between graft rejection and glomerulonephritis. At the sites of the deposits, the endothelial cells appeared enlarged and their nuclei protruded into the lumen. The findings are in line with the hypothesis that an antibody-mediated local activation of serum complement may be involved in the destruction of allografted tissue.


An ultrastructural study of the ciliary process in the rabbit following systemic administration of bacterial endotoxin.


Effect of chlorination in the final washer on bacterial counts of broiler chicken carcasses.


Transplants of glandular tissues for the development of tolerance in birds.


Lipoma of the oral cavity: literature review and case report.


Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II. The major DNA-synthesizing enzyme present in Pol A(1) (-)Escherichia coli (DNA polymerase II) has been purified to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme requires all four deoxynucleoside triphosphates, Mg(++), NH(4) (+), and native DNA for maximal activity. The enzyme activity is sensitive to sulfhydryl reagents and is insensitive to anti-DNA polymerase I antiserum. A second DNA-synthesizing enzyme, present in low amounts, has been identified in Pol A(1) (-) extracts. The relationship of this enzyme to DNA polymerases I and II is discussed.


Radioresistance of the enhancing effect of cells from carrier-immunized mice in an in vitro primary immune response. The in vitro primary response of mouse spleen cell suspensions to 2,4,6-trinitrophenyl(Tnp)-erythrocytes has been studied. The number of anti-Tnp plaque-forming cells that arise after antigenic stimulation in vitro is greatly enhanced by prior immunization in vivo with the carrier erythrocyte. The enhancement is antigen specific. The priming for an enhanced response can be elicited with very low antigen doses and is often apparent 24 hr after immunization. It is marked from day 3 to day 14. Spleen cells from carrier-primed mice will enhance the anti-Tnp response of normal cells when mixed cultures of the two cell populations are challenged with Tnp-erythrocytes in vitro. The carrier-primed cells mediating this enhancing effect are thymus derived. The development of the thymus-derived, carrier-specific cell population has been generally assumed to involve the antigenic stimulation of cell proliferation. It was, therefore, somewhat surprising to find that the enhancing effect of the carrier-primed cells, once they had been generated, is not inhibited by x-irradiation.


Isolation of transducing particles of phi-80 bacteriophage that carry different regions of the Escherichia coli genome. It has been possible to mate two strains harboring F-prime (F') factors and to isolate from such matings rare recombinants that behave as though the two episomes had fused. Thus, two genes not previously linked may be brought into close proximity. An F' factor carrying the attachment site for varphi80 was fused with one carrying the met-ppc-arg region of the chromosome. Lysogenization of such a strain, followed by induction, led to the isolation of varphi80arg(+) and varphi80met(+) transducing phages. This technique may be utilized as a general method for joining diverse bacterial genes to the genome of phage varphi80.


Conformational changes in ribosomes during protein synthesis. In a purified system containing poly(U) and ribosomal subunits from Escherichia coli, and purified transfer factors T and G, the active ribosomal complex passes through a cycle of contraction and expansion with the addition of each amino acid; aminoacyl-tRNA binding catalyzed by T produces the stable compact state, corresponding to the 70S conformation, whereas translocation with G expands the ribosome to a less stable 60S form. It is also shown that formation of the first peptide bond must be preceded by translocation with G. These findings are consistent with a model of chain initiation in the absence of initiation factors in which deacylated tRNA(Phe) bound to the P site signals translocation by G and GTP as soon as the 60S initiation complex has been converted to the 70S form by the enzymatic binding of Phe-tRNA.


Genetic recombination in Escherichia coli: the role of exonuclease I. The indirect suppression of recB(-) and recB(-)recC(-) mutations by the sbcB(-) allele is caused by the loss of a nuclease active on denatured DNA. Results from enzyme purifications and studies with a specific antiserum demonstrate that the activity present in sbcB(+) strains, and lost in sbcB(-) strains, is exonuclease I. It is likely that sbcB is the structural gene for exonuclease I. The loss of exonuclease I activity restores the recombination proficiency of Escherichia coli cells that has been lost by mutations in the recB and/or recC genes. This indicates that in the absence of the recB-recC-determined enzyme, exonuclease I prevents recombination. Hypothetical pathways illustrating this conclusion are presented.


Characterization of virulent bacteriophage infections of Escherichia coli in continuous culture.


Nucleotide sequence of the "denaturable" leucine transfer RNA from yeast. The nucleotide sequence of " denaturable"leucine acceptor transfer RNA (tRNA(Leu)(3)) from baker's yeast was determined on (32)P-labeled material. The molecule is 85 nucleotides long and can be folded into the "cloverleaf" model for secondary structure. The basis on which the sequence was deduced from the products of complete enzymatic digestion, prior to its unambiguous determination, is presented.