Electron microscopy of monkey kidney cell cultures infected with rubella virus. Two rubella virus strains isolated in this laboratory were investigated in terms of their growth in LLC-MK(2) cell cultures and their effect on cell morphology. Rubella virus grew readily in LLC-MK(2) cells, but cytopathic effects of the virus were not observed in infected cultures. Such infected cultures can be subcultured indefinitely and continue to shed virus. Examination of rubella-infected cell cultures by electron microscopy showed the presence of annulate lamellae in the cytoplasm of 15% of the cells. No changes were evident in the nuclei. These membranous inclusions varied in complexity from parallel arrays of annulate lamellae to large lamellar structures of complex morphology. An occasional cell contained a crystal lattice structure in association with the lamellae. Larger inclusions, consisting of disorganized arrays of "unit" membranes, were also found. Uninfected cells were devoid of annulate lamellae, crystals, and complex membranous inclusions. No viruslike particles were observed in any part of the cells from infected cultures. The significance of the structures observed has not been determined.


Properties of infectious T1 deoxyribonucleic acid. T1 deoxyribonucleic acid (DNA) infection of spheroplasts was characterized by the following. A small number of the DNA molecules initiated infectious centers, and a small number of the spheroplasts were infected by T1 DNA. Once a favorable encounter of T1 DNA with spheroplast occurred, a minimum of 20 to 30 min was required for T1 DNA to enter the spheroplast. The mature T1 particles produced in the infection of spheroplasts by T1 DNA were released in a burst, but the average burst size was quite small compared with a normal burst of the phage-infected bacteria. T1 DNA preparations, capable of causing viral growth in spheroplasts, did not require detectable amounts of protein for infectivity, were homogeneous in band and boundary sedimentation, and had a guanine plus cytosine content of 48% and a minimal molecular weight of 35 x 10(6). Denatured T1 DNA, like denatured lambdaDNA, did not infect spheroplasts. Renatured T1 DNA was not infectious; this was in marked contrast to renatured lambdaDNA.


Deoxyribonucleic acid replication and expression of early and late bacteriophage functions in Bacillus subtilis. The role of deoxyribonucleic acid (DNA) replication in the control of the synthesis of deoxycytidylate (dCMP) deaminase and lysozyme in Bacillus subtilis infected with bacteriophage 2C has been studied. These phage-induced enzymes are synthesized at different times during the latent period. It was shown by actinomycin inhibition that the formation of the late enzyme (lysozyme) required messenger ribonucleic acid (mRNA) synthesized de novo after the initiation of translation of mRNA which specifies the early function (dCMP deaminase). The inhibition of phage DNA synthesis by mitomycin C prevented the synthesis of lysozyme only when added before the onset of phage DNA replication, but it did not affect the synthesis or action of dCMP deaminase when added at any time during the latent period. Treatment of infected cells with mitomycin C after phage DNA synthesis had reached 8 to 10% of its maximal rate resulted in the production of normal amounts of lysozyme. These observations suggest that mRNA specifying early enzymes can be transcribed from parental (and probably also from progeny) DNA, whereas late functional messengers can be transcribed only after the formation of progeny DNA.


Infection of Mycobacterium tuberculosis with deoxyribonucleic acid extracted from mycobacteriophage B1.


Morphology of a virus of blue-green algae and properties of its deoxyribonucleic acid. The morphology of Safferman's virus of blue-green algae (phycovirus LPP-1) has been studied by electron microscopy and physicochemical methods. The virion has a short (100 to 200 A long, 150 A in diameter) forked tail, with an outer sheath, an inner core, and a capital attached to one of the vertices of a polyhedral head. The head capsid edge-to-edge distance is 600 A, based upon internal calibration of the magnification in electron micrographs by use of the line-line spacing of catalase crystals. Measurements of absorbancy and infectivity, and electron microscopy across the band of virus after zone centrifugation on a sucrose gradient, indicated that infectivity was correlated with the short-tailed particles described. The viral deoxyribonucleic acid (DNA) is linear, with a contour length of 13.2 +/- 0.5 mu, measured by the Kleinschmidt method. Its sedimentation coefficient, S(0) (20, w), is 33.4 +/- 0.7 S. These values are consistent with a molecular weight of 27 x 10(6) for the viral DNA. Based upon buoyant density in CsCl and thermal denaturation, the guanine-cytosine content of the DNA is 53%. The viral DNA was used as template for in vitro ribonucleic acid (RNA) synthesis by Escherichia coli RNA polymerase. This RNA annealed to 18% of the sequences in the viral DNA, 0.5% of the sequences in bacteriophage T7 DNA, and 0.25% of the sequences in Plectonema boryanum DNA, at saturating levels of RNA in the Hall-Nygaard hybridization assay. The lack of homology with T7 DNA is of interest because the two viruses are very similar morphologically. The lack of homology with host DNA suggests that this algal virus is a poor candidate for transduction.


Isolation of bacteriophages active against Neisseria meningitidis. Five distinct bacteriophages have been isolated from strains of Neisseria meningitidis. Filtrates with titers of 10(-4) to 10(-6) were produced with a modified Swanstrom and Adams semisolid agar procedure, employing Eugonbroth with added agar and an incubation temperature of 30 C. Of 49 strains of N. meningitidis (groups B and C), 25 were lysed by one or more of the phages, but there was no lysis of other Neisseria and Mima polymorpha strains.


Effects of nitroglycerin on capacitance vessels: a mechanism for reduction of left ventricular end-diastolic pressure.


Bilateral acoustic neuroma in a large kindred.


Purification and properties of a thermophilic bacteriophage lytic enzyme. A phage lytic enzyme was isolated from lysates of Bacillus stearothermophilus (NCA 1503-4R). The enzyme was purified 1,998-fold with a 27% recovery of enzyme activity. By use of polyacrylamide gel electrophoresis and sucrose gradient centrifugation the enzyme was judged free from protein contaminants. The lytic enzyme was active over a pH range of 6.0 to 7.0, with a maximum at 6.3, and it was stable between pH 7.0 and 8.0 and at 5.0 and unstable between pH 5.5 and 6.5. The temperature coefficient (Q(10)) was 2.27 between 35 and 45 C, 2.01 between 45 and 55 C, and 2.00 between 50 and 60 C. Lytic enzyme in 0.1 m sodium phosphate was not inactivated after a 1-hr exposure to temperatures below 65.5 C, whereas a 1% inactivation was observed at 70.6 C. A 2-hr exposure at 60.1, 65.5, and 70.6 C resulted in an inactivation of 1.2, 9.6, and 12.0%, respectively. A sodium phosphate concentration of at least 0.1 m was necessary for the prolonged exposure of lytic enzyme at 55 C (pH 6.3), whereas 0.005 m was required for maximal lytic activity. Lytic activity was stimulated 169, 165, and 160% by 10(-4)m Mg(++), Ca(++), and Mn(++), respectively. Lytic activity was inhibited 75% by 10(-4)m ethylenediaminetetraacetic acid (EDTA). The EDTA inhibition could be reversed by the addition of excess Mg(++), Ca(++), or Mn(++). Lytic activity was not affected by NaCl, KCl, or NH(4)Cl. Lytic activity was inhibited 100, 91, 25, 61, and 56% by 10(-4)m Hg(++), Cu(++), Zn(++), p-chloromercuribenzoate, and p-hydroxymercuribenzoate, respectively. Cysteine or 2-mercaptoethanol did not stimulate lytic activity, nor were these sulfhydryl compounds required for maintenance of enzyme activity during handling or storage. Cell walls were rapidly solubilized when incubated with lytic enzyme. Lytic action was complete after 1.5 min, with a 70% reduction in optical density (OD). Cell walls without lytic enzyme showed no reduction in OD during this period. The solubilization of N-terminal amino groups paralleled the reduction in OD and reached a level of 0.3 mumole/mg of cell wall after 4 min of incubation. Cell walls with and without lytic enzyme treatment showed a 3- and a 1.3-fold increase, respectively, in N-terminal amino groups after 3 hr of incubation. There was no release of reducing power in either the untreated cell wall suspensions or those treated with lytic enzyme. Electron micrographs of treated and untreated cell walls showed that the enzyme partially degrades the cell wall with the release of small wall fragments.


Inhibition of methanol and ethanol oxidation by pyrazole in the rat and monkey in vivo.


2-amino-4-hydroxyquinazolines as inhibitors of thymidylate synthetase.


F(ab')2-like fragments from severely burned patients provide a new serum immunoglobin component.


Initial characterization of a temperature sensitive mutant of monkey kidney cell.


Differences in Rh type between age groups of leukaemia patients.


Absence of the pigments of photosystem II of photosynthesis in heterocysts of a blue-green alga.


Hemagglutination assay for antigen and antibody associated with viral hepatitis. Hemagglutination assays are described for measuring hepatitis-associated Australia antigen and antibody. Red cells coated with isolated antigen, with chromic chloride as a coupling agent, are used for detection of antibodies. Detection of the antigen in serums depends on inhibition of hemagglutination. The test has the sensitivity and rapidity of the best tests available, is simpler to perform, and lends itself to large-scale screening of blood donors.


Fine structure of the exoerythrocytic stage of Plasmodium cynomolgi. The light microscopy of primate exoerythrocytic stages (liver stage) of malaria is well described. This report demonstrates the ultrastructure of the 7-day exoerythrocytic stage of Plasmodium cynomolgi in the liver of a rhesus monkey.


Herpesvirus neutralization: induction of the persistent fraction by insufficient antibody.


The relationship between cucumber necrosis virus and its vector, Olpidium cucurbitacearum.


Endocrine manifestations of neurofibromatosis in children.


Perforation of duodenal diverticula: a new hypothesis.


Mitomycin C (NSC-26980)--clinical brochure.


Preparation of a stable native control urine.


Anticoccidial drugs: factors affecting pathogenicity of avian coccidia.


Food additives and chemical carcinogens: on the concept of zero tolerance.


Effects of ultrasonics on the resolution of DNA in gel filtration.


The role of glucocorticoids in the regulation of thyroid function in man. The diurnal variation in thyroidal iodine release previously observed in euthyroid subjects appears to correlate with variations in serum immunoassayable thyrotropin (TSH). The hypothesis is advanced that this diurnal rhythm seems to be primarily regulated by a negative feedback action of circulating hydrocortisone. The administration of maintenance doses of hydrocortisone to patients with primary adrenal insufficiency and pharmacological doses to euthyroid subjects was accompanied by an acute suppression in both thyroidal iodine release and serum TSH values. An escape from glucocorticoid suppression was observed to occur in 2 or 3 days with the resumption of a near-normal thyroidal iodine release rate but was accompanied by a dampening or absence of the normal diurnal rhythm. Withdrawal of pharmacological doses of glucocorticoids in euthyroid subjects and maintenance doses in primary hypoadrenal patients was accompanied by transient stimulation of both serum TSH and thyroidal iodine release values. The study of a patient before and after cryohypophysectomy indicated that the rebound response in thyroid release after steroid withdrawal may be a useful testing procedure to indirectly assess the hypothalamicpituitary reserve capacity of TSH.


The effects of transport inhibitors on sodium outflux and influx in red blood cells: evidence for exchange diffusion. Active sodium transport (outflux or efflux) in red blood cells generally has been measured by assessing the amount of outflux inhibited by digitalis glycosides (outflux-fraction I). The presence of a ouabain-uninhibited sodium outflux (outflux-fraction II) attributable either to a second active transport mechanism or to exchange diffusion has been the subject of recent investigations. In the present study a variety of transport inhibitors, including ouabain, ethacrynic acid, furosemide, oligomycin, and amiloride, were studied for their effects on these components of sodium transport in RBC. In the presence of ouabain both ethacrynic acid and furosemide exerted similar effects on sodium outflux, inhibiting approximately 0.5 mmoles/L of cells per hr. This component of sodium outflux has been called outflux-fraction II. Ethacrynic acid showed no inhibitory potency when ouabain and furosemide were present, thereby suggesting that the same outflux component (fraction II) was affected by ethacrynic acid and by furosemide. In addition, furosemide reduced sodium influx to the same extent that it reduced sodium outflux. Outflux-fraction II, as defined by furosemide, did not contribute a net sodium outflux. These results of sodium outflux and influx experiments confirm the existence of a transport pathway which does not contribute to net flux and which fits the definition of exchange diffusion. The inhibitory effect of furosemide on outflux-fraction II remained despite the use of a sulfhydryl protective reagent, whereas the effect of ethacrynic acid was obliterated. No combination of inhibitors was found which affected the residual or uninhibited sodium outflux (0.4-0.5 mmoles/liter of cells per hr). Oligomycin possessed an inhibitory potency less than that of ouabain, and it exerted no effect on sodium outflux if it was superimposed upon ouabain inhibition. Amiloride proved to be a very weak inhibitor of sodium outflux in human erythrocytes.


N-substituted 2,2'-diphenamic acids and diphenimides.


Adenovirus DNA. II. Separation of molecular halves of adenovirus type 2 DNA.


Mechanism of the sensitization of bacterial transforming DNA to ultraviolet light by the incorporation of 5-bromouracil.


Incidence of aspergilloma in patients with pulmonary tuberculosis and hemoptysis.


Monitoring the fetal environment. 1. Biochemical methods.


Resistance of DNA against radiation-induced strand breakage in bacterial spores.


Physical studies of glycerol protection against x-ray inactivation of bacteriophage.


Determination of growth rates by tetracycline labeling in young guinea pig mandibles.


X-ray sensitivity and modifying effects of water in conidia of Aspergillus nidulans.


The biologic basis of radiologic findings in bone disease. Recognition and interpretation of abnormal bone architecture.


Atopic symptoms caused by tobacco hypersensitivity.


Ultrastructure of mammalian spermiogenesis. I. A tubular complex in developing sperm of the cottontop marmoset Sequinus oedipus.


Effects of trimeprimine (surmontil) on spermatogenesis and mood in normal volunteers.


Some physiological and biochemical aspects of host-parasite relations.


Influence of parenteral L-isomeric amino acids on absolute albumin synthesis rates in tropical sprue.


Effects of subclavian steal and compromised cephalic blood flow on cerebral circulation.


Binding of folic acid activity by body fluids.


The ratio between normal and sensitive erythrocytes in heterozygous glucose-6-phosphate dehydrogenase deficient women.


An evaluation of sternal tenderness as a sign of leukaemia.


A screening test for red blood cell catalase.


The evolution of the stress concept. Stress and cardiovascular disease.


The diagnosis of intracardiac metastasis of colon carcinoma by radioisotopic and roentgenographic studies.


Sequences of mother-infant behavior following a facial communicative gesture of pigtail monkeys.


A behavioral and neuropathological study of the sequelae of profound hypoxia.


[3H]harmaline distribution in monkey brain; pharmacological and autoradiographic study.


The effect of certain drugs on myocardial dynamics as recorded with the high-frequency (acceleration) direct body ballistocardiograph.


Multiple jejunal diverticula with volvulus.


Drug resistance in tuberculosis.

Drug resistance in tuberculosis.


The relation between the ultra-low frequency ballistocardiogram, the acceleration pneumocardiogram, and ascending aortic flow acceleration in the baboon.


Experimental cerebral schistosomiasis japonica in primates. Aspects of the pathogenesis of cerebral schistosomiasis have been elucidated. Arterial embolization alone, by either worms or eggs, does not appear to be likely as a major cause of the disease. When large numbers of eggs enter the brain, sensitization may play a role in the occurrence of neurological signs. These studies support but do not prove the theory that worms in the cerebral veins lay eggs that then cause the disease.


On the general problem of the estimation of therapeutic effects in man and the place of the Bcg in the detection of such effects.


Baboon into pig liver and kidney xenotransplantation.


Trimethoprim and sulphonamides: laboratory studies.


Some unresolved mysteries in the anatomy of the visual system.


Morphologic and immunologic changes induced in the lungs of rats by localized irradiation.


Gynecologic pathology in the Macaca mulatta. I.


Some effects of fetal and maternal hypophysectomy in pregnancy.


Immunoglobulin G levels and the titers of specific antiviral antibodies in amniotic fluid.


Improved lighting for endoscopic diverticulotomy.


Effect of ethylenediamine tetra-acetic acid (EDTA) on cholera and el tor vibrios.


Rickettsial vaccines.

Rickettsial vaccines.


Pseudomonas aeruginosa in respiratory illness: endogenous or exogenous?


Reduction in peroxide values and monocarbonyls of oxidized methyl oleate by several microbial cultures.


Rapid lysis of cell walls of Micrococcus radiodurans with lysozyme: effects of butanol pretreatment on DNA.


Serological relationships of endoplasm antigens of saprophytic mycobacteria.


Tyrosinase isoenzymes in six agaric species of Basidiomycetes.


A partial characterization of the alpha-toxin of Clostridium oedematiens type B.


Ward hostess experiment.

Ward hostess experiment.


Effect of lipaemia and acute haemodynamic disturbances on thrombus formation in rhesus monkeys.


Familial and hereditary anomalies producing brachydactylia of hands: roentgen manifestations.


Escherichia coli and Bacillus subtilis phage deoxyribonucleic acid-directed deoxycytidylate deaminase synthesis in Escherichia coli extracts.


Human carbonic anhydrases. IV. Properties of a mutant B type isozyme.


Current trends in treatment and prevention of meningococcal disease.


Evidence against the folate-mediated formylation of formyl-accepting methionyl transfer ribonucleic acid in Streptococcus faecalis R.


Antidotal action of new oximes in experimental organophosphate intoxication.


Giant calculus in a diverticulum of the male anterior urethra.


Kidney specific antigens in the urine of rats with experimental acute toxic nephropathy.


Mesonephric adenocarcinoma of the vagina in a 7-month-old infant simulating sarcoma botryoides. Successful control with supervoltage radiotherapy.


Activity of the reticuloendothelial system (RES) in early experimental thyroiditis.


Isolation of human serum low-density lipoproteins with the aid of an immune-specific adsorber.


Phylogeny of lipase specificity.

Phylogeny of lipase specificity.


Three common problems caused by drug-hormone interactions.


How metabolism affects clinical problems.


The serological differentiation of human blood stains from those of sub-human primates in Southern Africa using the anti-globulin inhibition technique.


The management of recurrences of malignant tumors of the uterine cervix by 42 MeV betatron.


What is drug resistance?

What is drug resistance?


Carcinoma occurring in pharyngoesophageal diverticulum: report of three cases.


Quantitative bacteriology in the clinical testing of new antituberculosis drugs.


A bacteriological and serological study of Mycobacterium avium and related strains.


Biological research in Schizophrenia. Report of a WHO scientific group.


Present-day communication in chemistry--problems and possibilities.


Chemical information services.

Chemical information services.


Theoretical aspects of communication in chemistry.


The "dokumentationsring der chemisch-pharmazeutischen industrie"; aims and methods.


CCBF--A system for the computer processing of chemical and biological facts.


The synthesis of succinate-dehydrogenase riboflavin.


Occurrence of N-glycolylmuramic acid in bacterial cell walls. A preliminary survey.


The heterogeneity of soluble estrogen receptors from rat uteri and their modification by temperature, imidazole compounds and estradiol.


Hodgkin's disease terminating in acute leukemia.


Cancer of the tongue: local control of the primary.


A case of diabetic ketosis presenting as an acute surgical abdomen.


Reduced infection hazard during mechanical ventilation.


Pulmonary findings associated with mitral stenosis.


An electroencephalographic study of nocturnal sleep in the epileptic patients.


Haemolytic activity of Russell's viper venom.


Immunisation of blood donors for the production of high titre anti-D antibody--a preliminary report.


In vitro sensitivity pattern of gram negative pathogens to nalidixic acid.


Newer techniques in protein isolation and characterization.


The occurrence of oxidase-positive non-gonococcal strains on Thayer-Martin selective media used in the laboratory diagnostic of N. gonorrhoeae.


Gentamicin in treatment of primary Gram-negative pneumonias.


Gonorrheal ophthalmia.

Gonorrheal ophthalmia.


Serum thyroxine resin uptake of liothyronine I 125, and free thyroxine index.


A cause of radioresistance in retinoblastoma: photoreceptor differentiation.


The sensory and motor role of impulses travelling in the dorsal columns towards cerebral cortex.


The cortico-striate projection in the monkey.


Complex Waldenström's syndrome associated with chronic lymphoid leukemia.


Red cell enzymes of the pongidae.

Red cell enzymes of the pongidae.


Role of monkeys' spatial preferences in performance of a nonspatial task.


Indigogenic methods for glycosidases. II. An improved method for beta-D-galactosidase and its application to localization studies of the enzymes in the intestine and in other tissues.


Stimulus attributes and training procedures in learning-set formation of Rhesus and Cebus monkeys.


Relative cost of medical care. OSMA presents medical cost figures in perspective before legislative study committee on consumer affairs.


Coal workers' pneumoconiosis in an affluent society.


Attitudes toward Medicare among older people.


Participation of low-income urban women in a public health birth control program. 564 low-income nonwhite mothers (median age 22 years) were evaluated by the District of Columbia Health Services Administration between November 1964 and December 1965 to determine, among other things, 1) the extent of their continuing  participation in a birth control program, 2) birth reduction due to the program, and 3) reasons for discontinuation of birth control.  During 70% of the almost 13,000 woman-months involved in the study, the women practiced birth control.  At 2 years postpartum, 67% were using contraceptives, 28% had used contraceptives but were not currently using them, and 5% had never used any contraceptive.  The percentage of women using contraceptives increased slightly with parity; women with 4 or more children were the largest proportion of these using contraceptives.  The consistent use of birth control methods varied depending on marital status and age.  73% of the women selected oral contraceptives as their first choice, 7% selected intrauterine devices, and 8% selected foam.  The Pearl pregnancy rate was 19.3 per 100 women-years.  There was an estimated 55% reduction in the number of expected births during the study period.  The 158 women who were no longer using birth control methods gave these reasons for stopping: 1) pregnancy, 2) separation from husband, 3) surgery, 4) illness, 5) weight gain, 6) nervousness, and 7) too much trouble.


A procedure for examining time changes in rates of readmission to psychiatric hospitals.


The epidemiology of accidents among the Navajo Indians.


Hearing conservation in the U.S. Army.

Hearing conservation in the U.S. Army.


Response to physical activity programs and their effects on health behavior.


Decline in statewide mobile x-ray programs to detect tuberculosis.


Evaluation of the decentralization process by employees of a state health department.


Background and methodology of a study of congenital malformations.


Take rates by double versus single insertions of smallpox vaccine in revaccinees.


Residency training in public health and general preventive medicine.


Discharge of intact vacuoles from HeLa cells on exposure to antibody and complement.


Behavior of previously frozen erythrocytes used during open-heart surgery.


Microplate system for routine use in blood bank laboratories.


An anti-Diego (Dib) antibody occurring during pregnancy.


Cytokinetics of argentaffin cells in mouse intestinal mucosa.


Fixation of atmospheric nitrogen by the strains of Arthrobacter.


Focal dermal hypoplasia. Ocular and general manifestations with a survey of the literature.


Vaccination for Pseudomonas aeruginosa.

Vaccination for Pseudomonas aeruginosa.


Instability of the hip in the newborn. Classification for selective treatment; pathogenesis of the dislocation and complications.


Studies on pharmacological enhancement of blood fibrinolytic activity.


Anti anginal drugs, their evaluation by double blind trial.


Ion binding of penicillins to proteins.

Ion binding of penicillins to proteins.


Maternal Rh antibody and delayed neonatal expression of Rh antigen.


Therapy of childhood seizures.

Therapy of childhood seizures.


Ethacrynic acid in the out-patient management of cardiac edema.


Ancillary use of phenazopyridine (Pyridium) in urinary tract infections.


A comparative study of molindone and trifluoperazine.


Antagonistic effect of propranolol upon secondary actions of phenmetrazine.


Clinical pharmacologic study of an isoquinoline derivative (EN 1661) and of its ability to induce fibrinolytic activity in the circulation of man.


Above all-the cancer patient. A personal tribute to Roald N. Grant, M.D. (1912-1970).


The management of patients with metastatic bone cancer.


The prognosis and insurability of thyroid cancer patients.


Characterization of glucagon-like immunoreactivity (GLI).


The potential of negative pions in the therapy of cancer.


Unproven methods of cancer treatment. Hemacytology index (HCI).


Molecular size of extractable glucagon and glucagon-like immunoreactivity (GLI) in plasma.


Mode of action of a diazoxide analog (A025). Its effects on insulin secretion.


Type-C particles in human tissues. II. Electron microscopic study of embryonic cultures infected with a murine leukemia virus.


Bacillus subtilis glutamine synthetase. Purification and physical characterization.


Some kinetic properties of Bacillus subtilis glutamine synthetase.


Properties of a dicarboxylic amino acid transport-deficient mutant of Streptococcus faecalis.


Multiple transport components for dicarboxylic amino acids in Streptococcus faecalis.


The diagnosis and management of Hodgkin's disease: current concepts.


Fracture-dislocations of the ankle joint with impaction injury of the lateral weight-bearing surface of the tibia.


Biosynthesis of low density lipoprotein by cell-free preparations of rat intestinal mucosa.


Central acetabular fracture secondary to epileptic seizure.


Nerve growth factor and nerve growth-stimulating activity in sera from normal and neuroblastoma patients.


The effects of betahistine on the isolated perfused baboon kidney.


Role of parenteral hyperalimentation in management of multiple catastrophic complications.


Serological properties of Pseudomonas aeruginosa. II. Type-specific thermolabile (flagellar) antigens.


Isolation and characterization of some newly isolated B. cereus phages.


Induction of membrane-bound penicillinase synthesis in B. cereus 569.


Electron microscopic comparison of Babesia spp. and hepatic changes in ponies and mice.


Neurophysiologic and behavioral effects of certain anesthetics administered intramuscularly in the rhesus monkey (Macaca mulatta).


Immunoserologic properties of antigens from Vibrio fetus of ovine origin.


Effects of 20 years' storage on lyophilized cultures of bacteria, molds, viruses, and yeasts.


Meniere's disease. (Preliminary report of a new treatment).


Sensitivity and resistance of a blue-green alga Phormidium mucicola to streptomycin and penicillin.


Studies on live attenuated mumps virus vaccine. II. Biological characteristics of the strains adapted to the amniotic and chorioallantoic cavity of developing chick embryos.


Studies on live attenuated mumps virus vaccine. 1. Attenuation of mumps virus by serial passage in the chorioallantoic cavity of developing chick embryos and field trials by the inhalation method.


A specific inhibitor of megacin A from Bacillus megaterium producing megacin A.


Hemolysins and related enzymes of Vibrio parahaemolyticus. I. Identification and partial purification of enzymes.


Location and action of Streptomyces griseus alpha-D-mannosidase, an antibiotic-transforming enzyme.


Proteases of the genus Bacillus. II. Alkaline proteases.


Purification and characterization of the amylase of B. subtilis NRRL B3411.


Immunoglobulins in chronic cold haemagglutinin disease.


Histochemical study of the fructose-1,6-diphosphate aldolase in parenchymal cell damage of the liver of rats fed on 4-dimethylaminoazobenzene.


25 years of impetuous upsurge.

25 years of impetuous upsurge.


Changes in the lipolytic activity of the arterial wall in albino rats. IV. Effect of glucose.


Experimental investigations on the microclimate of tobacco fermentation chambers. IV. Histochemical changes in the catecholamines of the medullar part of the suprarenals in rabbits.


Changes of temperature of the skin in patients with eczema under the effect of treatment with corticosteroids.


About the solitary cysts of kidney.

About the solitary cysts of kidney.


An experiment for the use of a nonsymmetric multivibrator as a generator for sound anesthesia.


Purification and some properties of the extracellular protease of Bacillus pumilus.


Neurofibroma and leprosy.

Neurofibroma and leprosy.


The lipids of Mycobacterium tuberculosis BCG: fractionation, composition, turnover and the effects of isoniazid.


Feed intake of goats during volatile fatty acid injections into four gastric areas.


Treatment of malleolar fractures.

Treatment of malleolar fractures.


Nomarski differential interference contrast--fluorescence microscopy: a new technique designed to improve resolution in fluorescent specimen.


The effect of glucagon on plasma calcium ion activity in the monkey.


Behavioral changes following repeated low doses of monomethylhydrazine.


Tolerance of rhesus monkeys to pCO2 of 195 mm. Hg at 0.5 atmosphere total pressure.


Brain study in balloon-borne monkeys exposed to cosmic rays.


Evaluation of phosphonomycin, a new cell wall-active antibiotic.


Pharmacology of polyriboinosinic: polyribocytidylic acid, a new antiviral and antitumor agent.


Pyocin antibiosis in chick embryos.

Pyocin antibiosis in chick embryos.


Successful pregnancy in a patient with Ebstein's anomaly. A case is described of a successful pregnancy in a woman with Ebstein's anomaly. It is suggested that careful assessment should be made before advising against starting or terminating pregnancies, since successful outcomes are possible.


Isolation of spirochaetes from primates and other mammalian species.


Serology of normal primates.

Serology of normal primates.


Identification of the asymptomatic female carrier of N. gonorrhoeae. Treatment with ampicillin.


Gonococcal penile ulcer.

Gonococcal penile ulcer.


Acid production in diabetic acidosis; a more rational approach to alkali replacement. The production of organic acids in severe diabetic acidosis was studied to determine the contribution of various acids and to reassess alkali requirements. In 11 patients the mean total concentration of determined organic acids was 16 mEq/l., while the mean estimated base deficit was 24 mEq/l. Acetoacetic and beta-hydroxybutyric acids accounted for 75% of measured organic acid. In 10 patients the mean amount of sodium bicarbonate administered for correction of the acidosis was 185 mEq, while the mean requirement was 394 mEq.These findings imply that the methods commonly used to determine the base deficit and the alkali requirements in patients with diabetic acidosis may be invalid. The prompt administration of alkali should be limited, and we suggest that the blood pH should be restored only to 7.25.


Changes in gut flora after cephalexin treatment. Eighteen patients with urinary tract infection were treated with cephalexin orally. Absorption was variable, between 29 and 89% of the total daily dose being excreted in the urine in 24 hours. A significant number of patients became faecal carriers of Pseudomonas aeruginosa compared with a control group who received no antibiotics. Four of the cephalexin-treated patients acquired a strain of Ps. aeruginosa known to be present in food from the hospital diet kitchen and one developed a urinary tract infection with this strain.


Toxocariasis.

Toxocariasis.


Hemolytic disease of the newborn. A new law for prenatal blood test and disease reporting.


Professor Emil Koen (on the occasion of his 60th anniversary).


Plasmatic and cellular antituberculous factors in the blood of patients with carcinoma and of patients with other diseases.


On the changes of the arterial wall in rabbits after trauma.


The effect of magnesium gluconate on experimental atherosclerosis in pigeons, and on the content of sulfhydryl and disulfide groups in aorta and liver.


Methods for the investigation of the nucleotide compositon of DNA in leptospirae.


Clinical and biochemical changes in patients after resection of the stomach according to Billroth II.


Narcohypnosis with trilene.

Narcohypnosis with trilene.


About the development of sclerosis of the thyroid gland in struma (immunomorphological investigation).


Radioactivity of drugs, infusions and decoctions.


Radioactive strontium (85Sr) in the diagnostics of metastatic tumors in bones.


The value of chlamydospore test in the identification of Candida albicans.


Comparative investigations of the phase analysis between bundle-branch heart block and Wolff-Parkinson-White syndrome.


Results of the intraoperative renal biopsy and the bacterial finding in the urine in nephrolithiasis.


The interaction of normal lymphocytes and cells from lymphoid cell lines. I. The nature of the activation process.


Properties of protective malarial antibody.


Cytoplasmic control over the nuclear events of cell reproduction.


Coordination of the rhythm of beat in some ciliary systems.


Ultrastructural observations on four species of sporozoa.


Studies on the protein composition of extracts of Schistosoma mansoni cercariae.


Some observations on the transmission of Plasmodium inui.


Monkey to man transmission of Plasmodium falciparum by Anopheles freeborni mosquitoes.


Relationship between detectable microfilaremia and total population of microfilariae in monkeys infected with Dirofilaria corynodes.


Circulating precipitating antibodies in the rabbit from the bites ofRhodnius prolixus as shown by agar-gel tests.


The effect of suramin on experimental Trypanosoma cruzi infection in mice.


A personal post from Rio.

A personal post from Rio.


Altered redox state obscuring ketoacidosis in diabetic patients with lactic acidosis.


"Host" antigens associated with schistosomes: observations on their attachment and their nature.


Damage to adult Schistosoma mansoni after transfer to a hyperimmune host.


The intranuclear metabolism of testosterone in the accessory organs of reproduction.


The isolation of antilymphocytic antibodies.


Comparative immunochemical studies of the toxic culture filtrates and endotoxin preparations from serologically identical strains of Escherichia coli 2796-0129 and Shigella flexneri 5.


Hormone action at the membrane level. I. Properties of adenyl cyclase in isolated plasma membranes of rat liver.


The use of easily obtained pre-coded data in screening applicants to a long-term care facility.


Immunologic detection of single-stranded DNA in adeno-associated satellite virions.


Persistent lymphocytic choriomeningitis viral infection. 3. Virus-anti-viral antibody complexes and associated chronic disease following transplacental infection.


Studies of maleylated chains of human IgM.


A cytolytic system for the in vitro detection of cell-mediated immunity to soluble antigen.


Hidden rheumatoid factors reacting with "non a" and other antigens of native autologous IgG.


Protein A from Staphylococcus aureus. X. Complement-fixing activity of complexes between protein A and myeloma IgG or papain fragments of IgG.


Characterization of a monoclonal canine macroglobulin.


Degradation of immunoglobulin G by lysosomal acid proteases.


Besnoitia jellisoni (Sporozoa: Toxoplasmea) in rodents from Utah and California.


Counting of Schistosoma mansoni eggs in feces. Comparison of a filtration technique and a dilution technique.


Immunodiffusion studies on a skin-inhabiting Leishmania from the tropical porcupine, Coendou rothschildi Thomas.


Phenoxymethylpenicillin (penicillin V) and phenethicillin.


Comparative serological reactivities of twenty-seven polysaccharides from nine species of dermatophytes.


Is uncertainty a key to neurotigenesis?

Is uncertainty a key to neurotigenesis?


Zinc, copper and manganese in the alimentary tract of sheep.


Distribution of the ABO and Rh D blood groups in patients with leukemia.


Urocanase and N-formimino-L-glutamate formiminohydrolase of Bacillus subtilis, two enzymes of the histidine degradation pathway.


Sequential synthesis of histidine-degrading enzymes in Bacillus subtilis.


Genetic basis of histidine degradation in Bacillus subtilis.


Interconversion of alpha- and gamma-penicillinase from Bacillus cereus 569.


Biohydrogenation of unsaturated fatty acids. IV. Substrate specificity and inhibition of linoleate delta-12-cis, delta-11-trans-isomerase from Butyrivibrio fibrisolvens.


Biochemical studies of bacterial sporulation and germination. 23. Nucleotide metabolism during spore germination.


Lower gastrointestinal hemorrhage secondary to enteric neurofibromatosis (Recklinghausen's disease).


Lipid peroxide appearance in microsomal fraction.


The synthesis of pyoluteorin.

The synthesis of pyoluteorin.


Serum complement (C'3) levels in patients with autoimmune bullous skin diseases.


Café au lait spot in neurofibromatosis and in normal individuals.


Measurement of glomerular filtration rate using a single injection of 51Cr-Edetic acid.


Ultrasonic visualization of ultrasonically produced lesions in brain.


Occupational asthma. I.

Occupational asthma. I.


Extracellular concentrating of proteins in the cecropia moth follicle.


Kidney transplantation and long-term dialysis.


The embryolethal and teratogenic effects of cyclophosphamide on mouse embryos.


The effects of storage and heparin on the activity of serum complement, with particular reference to the detection of blood group antibodies.


Anaphylactic reactions to IgA: a difficult transfusion problem.


Comparison of immunoglobulin analyses of antemortem and postmortem sera.


Obstetric and gynecologic considerations of dwarfism.


Yeasts septicemia and endocarditis. Mycological, immunological and therapeutical aspects.


Pathophysiology of endotoxin shock in the primate.


Canditoxin, a new toxic substance from a strain of Candida albicans.


Immunological properties of polysaccharides and polysaccharide-protein-complexes of C. albicans.


Ethylenediamine tetraacetic acid. A new biological preservative for beer.


Peptidase activity of yeast plasmocoagulase.


Carbonic anhydrase of microorganisms. I. An enzyme from baker's yeast which catalyzes the formation of carbamate from ammonium bicarbonate solutions.


Activation of prephenoloxidase. II. Activation by alpha-chymotrypsin.


An enzymatic and immunological comparison of two proteases from a transformable Bacillus subtilis with the "subtilisins".


Purification and properties of follicle-stimulating and luteinizing hormones from horse pituitary glands.


Properties of flagellin from Bacillus megaterium KM and its association with the cytoplasmic membrane.


The response of mice to the intravenous injection of lymphocytic choriomeningitis virus.


Studies in bioluminescence. IV. Properties of luciferin from Pholas dactylus.


Lithium carbonate in the treatment and prophylaxis of recurrent affective disorders: long term follow-up.


The mapping of genes for spore formation on the chromosome of Bacillus licheniformis.


Preparation and properties of diphtheria toxoids in submerged culture. IV. A revised method for the purification of diphtheria toxin.


Growth inhibition of bacterial variants.


Proteins of the murine C-type RNA tumour viruses: isolation of a group-specific antigen by isoelectric focusing.


Ethylene oxide sterilant residues.

Ethylene oxide sterilant residues.


A comparative study of red clover vein mosaic virus and some other plant viruses.


Experimentally induced ethanol dependence in rhesus monkeys.


Structure of Bacillus subtilis bacteriophage phi25 and phi25 deoxyribonucleic acid. The structure of Bacillus subtilis bacteriophage phi25 and phi25 deoxyribonucleic acid (DNA) were studied by electron microscopy. The head of phi25 is a regular polyhedron measuring 75 nm in diameter. The uncontracted tail of phi25 is 130 nm in length and includes a large, complex tail plate. Phage phi25 DNA is double-stranded and has a molecular weight of approximately 100 million as determined by electron microscopic length measurements and analytical band sedimentation in CsCl. The complementary strands of phi25 DNA contain numerous random interruptions. Chemical analysis of phi25 DNA demonstrated that 5-hydroxymethyluracil replaces thymine and that the DNA has a mole per cent (guanine plus cytosine) of 42.


Morphology and physiology of the intracellular development of Bacillus subtilis bacteriophage phi25. The morphology of the intracellular development of bacteriophage phi25 in Bacillus subtilis 168M has been correlated with nucleic acid synthesis in infected cells. Host deoxyribonucleic acid (DNA) synthesis was shut off by a phage-induced enzyme within 5 min after infection, and another phage-mediated function extensively degraded host DNA at the time of cell lysis. Synthesis of phage DNA in infected cells began within 5 min and continued until late in the rise period. After phage DNA synthesis and coinciding with lysis, much of the unpackaged, newly synthesized phage DNA was degraded. Studies of thin sections of phi25 infected cells suggested that unfilled capsids may be precursors to filled capsids in the packaging process. To assess dependence of capsid formation on phage DNA replication, cells were either treated with mitomycin C and infected with normal phage or infected with ultraviolet-irradiated (99% killed) phi25. Only empty capsids were found in these cells, indicating that capsid production may be independent of the presence of newly synthesized viral DNA.


Genetic map of bacteriophage alpha.

Genetic map of bacteriophage alpha. Temperature-sensitive mutants of phage alpha were obtained by means of various mutagens and assigned to 25 complementation groups. Temperature-sensitive mutants belonging to 21 complementation groups and a mutant giving turbid plaques were used to perform two- and three-factor crosses. Seventeen of the cistrons and the turbid mutant were shown to belong to the same linear linkage group, which showed no signs of circularity. The remaining four unlinked cistrons showed peculiarities in their recombination properties. Genes which are known to be expressed earlier apear to be grouped together in a terminal segment of the linkage group.


Characterization of the acute hepatic failure syndrome associated with the anhepatic state.


The preparation of a high calorie nutrient solution.


Effects of an oral interferon-inducer on the hematopoietic and reticuloendothelial systems.


Experimental immunisation of sows against transmissible gastroenteritis. I. Immunisation with a bacteria-free suspension of transmissible gastroenteritis virus prepared from the intestines of infected pigs.


The epidemiology of wildlife rabies.

The epidemiology of wildlife rabies.


The eye and adnexal disease of the Alaska native-1970.


Thalamic sensory nuclear ablations in trained monkeys.


Inhibition of prostaglandin biosynthesis by eicosa-5,8,11,14-tetraynoic acid.


Gut flora after cephalexin.

Gut flora after cephalexin.


Diabetic acidosis.

Diabetic acidosis.


Diabetic emergency.

Diabetic emergency.


Diabetic ketoacidosis during the influenza epidemic. During eight weeks of a recent influenza epidemic 29 patients with ketoacidosis were admitted to the General Hospital, Birmingham. This was an exceptionally large number of cases. Of these, 14 had complained of a cough, nine had clinical evidence of respiratory infection, and four extensive bronchopneumonia. Hypokalaemia was present on admission in several instances and caused respiratory failure and death in three patients. Since the dangers of initial hypokalaemia are increased during the treatment of ketoacidosis, especially when sodium bicarbonate is used, serum potassium levels must be estimated initially and, if necessary, potassium chloride given intravenously at more frequent intervals than usual.


A study of the heterogeneity of anti-dinitrophenyl (DNP) antibodies using inhibition of bacteriophage neutralization.


Effect of polyspecific rabbit anti-immunoglobulin antisera on human lymphocytes in vitro.


Reactivities to horse anti-lymphocyte globulin. I. Induction of immunologic tolerance in man.


Reactivities to horse anti-lymphocyte globulin. II. Serum sickness nephritis with complement alterations in man.


Immunoglobulin A in the pig. II. Sow's colostral and milk IgA: quantitative studies and molecular size estimation.


Accessory oculomotor nuclei in the monkey: projections and effects of discrete lesions.


The cerebellar nuclei of macaca mulatta: a morphological study.


Subcortical projections, cortical associations, and some intrinsic interlaminar connections of the striate cortex in the squirrel monkey (Saimiri).


Pharyngeal fistulae after laryngectomy.

Pharyngeal fistulae after laryngectomy.


Choline acetyltransferase activity of human brain tissue during development and at maturity.


Vasospasm in response to repeated subarachnoid hemorrhages in the monkey.


Angiomyolipoma: long-term cure following radical approach to malignant-appearing benign intraspinal tumor. Report of three cases.


The role of monoamines in the hypothalamus for temperature regulation.


The mammalian subfornical organ.

The mammalian subfornical organ.


Ependymal secretion, especially in the hypothalamic region.


Intravenous diazepam and its anticonvulsive action.


Indicator dilution studies in the small bowel of patients with cholera diarrhea. II. Comparisons of absorbable and "poorly absorbable" substances.


Unusual membranous body in the spermatids of pig tail monkey.


Anthropological studies of South-Sinai Bedouins.


Renal transplant in Singapore.

Renal transplant in Singapore.


Uptake of histamine by rabbit platelets incubated in plasma or salt solutions.


The purification and properties of a ribonucleoenzyme, o-diphenol oxidase, from potatoes. 1. o-Diphenol oxidase was isolated from potato tubers by a new approach that avoids the browning due to autoxidation. 2. There are at least three forms of the enzyme, of different molecular weights. The major form, of highest molecular weight, was separated from the others in good yield and with high specific activity by gel filtration through Bio-Gel P-300. 3. The major form is homogeneous by disc electrophoresis but regenerates small amounts of the species of lower molecular weight, as shown by rechromatography on Bio-Gel P-300. 4. There is an equal amount of RNA and protein by weight in the fully active enzyme. The RNA cannot be removed without loss of activity, and is not attacked by ribonuclease. 5. The pH optimum of the enzyme is at pH5.0 when assayed with 4-methylcatechol as substrate. It is ten times more active with this substrate than with chlorogenic acid or catechol. The enzyme is fully active in 4m-urea. 6. A minimal molecular weight of 36000 is indicated by copper content and amino acid analysis of the protein component of the enzyme. 7. The protein contains five half-cystinyl residues per 36000 daltons, a value similar to that found in o-diphenol oxidase from mushrooms. It also contains tyrosine residues although, when pure, it does not turn brown by autoxidation.


A factor in animal tissues that causes sialyl residues of mucoproteins to react as free sialic acid in the Warren assay. 1. The mucin of the Cowper's gland of the boar is a sialomucoprotein similar to submaxillary-gland mucin. When a solution of either mucin has been incubated for 5min or less with a particulate fraction from homogenized uterine endometriumplus-myometrium of the rabbit, 10-20% of sialyl residues (N-acetylneuraminic acid) give a positive Warren reaction for free N-acetylneuraminic acid. The particulate fraction is devoid of neuraminidase and no free (diffusible) N-acetylneuraminic acid appears during incubation. The factor that catalyses the formation of directreading non-diffusible N-acetylneuraminic acid occurs also in liver, kidney and intestinal mucosa of the rabbit. The factor is present in very small (;microsomal') particles and has not yet been solubilized. Homogenates of boar Cowper's gland contain both factor and mucin; thus direct-reading non-diffusible N-acetylneuraminic acid appears when such homogenates are stored. 2. Under optimum conditions 1mg of uterine protein catalyses the formation of 0.05-0.1mumol of direct-reading non-diffusible N-acetylneuraminic acid/min. This activity is considerably higher than the neuraminidase activities of comparable homogenates of animal tissues or of liver lysosomes. The factor is thermostable and its activity shows little variation within (i) the pH range 3-10, (ii) the temperature range 20-37 degrees C. Activity is inhibited strongly by 2,2'-bipyridyl and by ammonium pyrrolidine dithiocarbamate but is unaffected by EDTA. Its action can be simulated by low concentrations of Fe(2+). From this it may be inferred that the factor is a protein-bound from of bivalent iron. A number of pure iron-containing proteins and haemoproteins were completely inactive. The following substrates were not sources of direct-reading non-diffusible N-acetylneuraminic acid: methoxyneuraminic acid, sialyl-lactose, brain gangliosides, and sialoproteins in which N-acetylneuraminic acid is linked to galactose residues. 3. It is proposed that the factor (or Fe(2+)) reacts with the mucin in a manner that renders the C-4-C-5 bond of sialyl residues susceptible to periodate oxidation.


Galactothermin, a reversibly heat-precipitable protein of human milk at neutral pH. 1. A protein, aggregating at body temperature and solubilizing when cooled, was isolated from fresh human milk at neutral pH and studied for some of its physical, chemical and immunological properties. The name ;galactothermin' is proposed for this protein. 2. Isolation and purification of galactothermin involved casein removal from skim milk at pH4.64 followed by centrifugal fractionation of residual protein-containing solutions repeatedly heated and cooled between 40 degrees C and 0 degrees C at pH7.3. 3. The molecular weight by ultracentrifugal analysis and the minimum molecular weight by sum of amino acid residues were 11400 and 14000 respectively. The sedimentation coefficient s(25,w) was 1.05S and the diffusion coefficient was 7.15x10(-7). Reversible aggregation is favoured by increase in protein concentration, ionic strength, temperature, time and approach to the isoionic point of 7.27 from either acidic or alkaline conditions. 4. Among the amino acid residues, proline predominates and non-polar species account for two-thirds of the total. Cysteine and cystine are absent. Analysis of galactothermin showed it to be essentially free of hexose, sialic acid, calcium and phosphate. 5. Galactothermin is antigenic in the rabbit as evidenced by the passive cutaneous anaphylaxis test. Single precipitin lines are produced in immunodiffusion tests. 6. By electrophoresis in polyacrylamide gel at pH4.0 only one sharp band is produced.


The fate of sulphadimethoxine in primates compared with other species. 1. The metabolism of sulphadimethoxine (2,4-dimethoxy-6-sulphanilamidopyrimidine) was examined in nine species of primates and nine species of non-primates. 2. The main metabolite of the drug in the urine in man, rhesus monkey, baboon, squirrel monkey, capuchin, bushbaby, slow loris and tree shrew was sulphadimethoxine N(1)-glucuronide. In the green monkey, although the main metabolite was N(4)-acetylsulphadimethoxine, the N(1)-glucuronide was also a major metabolite. 3. In the dog, rat, mouse, guinea pig, Indian fruit bat and hen the N(1)-glucuronide was a minor metabolite in the urine, whereas in the cat, ferret and rabbit this glucuronide was not found in the urine. 4. All the species examined except the dog excreted some N(4)-acetylsulphadimethoxine, which was the major metabolite in the green monkey, rabbit and guinea pig. 5. In the tree shrew, a doubtful primate, N(1)-glucuronide formation was similar to that in the other primates. 6. It is suggested that the slow excretion of the drug by the rat may be due partly to strong binding of the drug to tissue proteins and that the strength of binding may vary with species. 7. In the rat the amount of N(1)-glucuronide found in the urine is not a true indication of the extent of this conjugation since much more of the conjugate was found in the bile (7% of the dose) than in the urine (1%). In the rabbit, no N(1)-glucuronide was found in the bile or urine, but a small amount of sulphadimethoxine N(4)-glucuronide was found in the bile of the rat (0.5% of dose) and rabbit (0.8%).


The fate of benzoic acid in various species. 1. The urinary excretion of orally administered [(14)C]benzoic acid in man and 20 other species of animal was examined. 2. At a dose of 50mg/kg, benzoic acid was excreted by the rodents (rat, mouse, guinea pig, golden hamster, steppe lemming and gerbil), the rabbit, the cat and the capuchin monkey almost entirely as hippuric acid (95-100% of 24h excretion). 3. In man at a dose of 1mg/kg and the rhesus monkey at 20mg/kg benzoic acid was excreted entirely as hippuric acid. 4. At 50mg/kg benzoic acid was excreted as hippuric acid to the extent of about 80% of the 24h excretion in the squirrel monkey, pig, dog, ferret, hedgehog and pigeon, the other 20% being found as benzoyl glucuronide and benzoic acid, the latter possibly arising by decomposition of the former. 5. On increasing the dose of benzoic acid to 200mg/kg in the ferret, the proportion of benzoyl glucuronide excreted increased and that of hippuric acid decreased. This did not occur in the rabbit, which excreted 200mg/kg almost entirely as hippuric acid. It appears that the hedgehog and ferret are like the dog in respect to their metabolism of benzoic acid. 6. The Indian fruit bat produced only traces of hippuric acid and possibly has a defect in the glycine conjugation of benzoic acid. The main metabolite in this animal (dose 50mg/kg) was benzoyl glucuronide. 7. The chicken, side-necked turtle and gecko converted benzoic acid mainly into ornithuric acid, but all three species also excreted smaller amounts of hippuric acid.


The wall content and composition of Bacillus substilis var. niger grown in a chemostat. Bacillus subtilis var. niger was grown in a chemostat with various growth limitations and at various growth rates. The wall content and composition of the organism grown under these conditions were determined. The wall content, expressed as a percentage of the dry weight of organisms, varied with the growth rate. Analysis of wall samples showed that their composition also varied, particularly with respect to the phosphorus content. Wall samples extracted with trichloroacetic acid under carefully controlled conditions were found to contain various amounts of phosphorus, this being present as a glycerol phosphate polymer containing hexose (glucose and in some cases galactose), i.e. a teichoic aid. Teichoic acids were present in the walls of organisms grown under all conditions except when phosphorus limited growth. Then a different anionic polymer, composed of glucuronic acid and N-acetylgalactosamine (a teichuronic acid), was present. Under the specific growth conditions at pH7.0 and 35 degrees C in a chemostat, teichoic acid and teichuronic acid appeared to be mutually exclusive.


Purification and properties of two extracellular beta-lactamases from Bacillus cereus 569-H. 1. When Bacillus cereus 569/H was grown in a casamino acid (casein-hydrolysate) medium containing zinc sulphate rapid production of extracellular beta-lactamase II preceded that of beta-lactamase I. 2. beta-Lactamase I was separated from beta-lactamase II by fractional precipitation with ammonium sulphate. 3. beta-Lactamase I was purified by a process involving chromatography on Celite and DEAE-cellulose and beta-lactamase II by chromatography on DEAE-cellulose after denaturation of beta-lactamase I by heat. Both enzymes were obtained in crystalline form. 4. beta-Lactamase II prepared in this way appeared to have a higher molecular weight than beta-lactamase I and required Zn(2+) as a cofactor for both cephalosporinase and penicillinase activities.


Membrane-active antibacterial compounds.


Von Recklinghausen's disease and melanoma of the uvea.


The composition of beta-lactamase I and beta-lactamase II from Bacillus cereus 569-H. 1. Crystalline beta-lactamase I from Bacillus cereus 569/H yielded only amino acids on acid hydrolysis, but crystalline beta-lactamase II from the same organism yielded also substantial quantities of neutral sugars and amino sugars. 2. Analysis with an amino acid analyser indicated that the two enzymes were similar though not identical in overall amino acid composition. Analysis of neutral and amino sugars as their silyl derivatives by gas-liquid chromatography showed that the carbohydrate moiety of beta-lactamase II contained residues of glucose, galactose, mannose, fucose, glucosamine and galactosamine. 3. After oxidation and hydrolysis both beta-lactamases gave small amounts of cysteic acid. After treatment of inactive Zn(2+)-free beta-lactamase II with N-ethylmaleimide or iodoacetate enzymic activity was not restored by the addition of Zn(2+).


A note on salmonella infections in wild animals in Khartoum, Sudan.


Activity of single cells in the osmoreceptor-supraoptic nuclear complex in the hypothalamus of the waking rhesus monkey.


Role of the sympathetic nervous system in daunomycin-induced arrhythmia in the monkey. 1. Infusion of daunomycin 50 mg/kg in the monkey consistently induced ventricular arrhythmias which were not influenced by bilateral vagotomy.2. A central sympathetic component to the arrhythmias was suggested because spinal transection, ganglionic blockade or bilateral stellate ganglionectomy prevented any alterations in the e.c.g.3. Bilateral adrenalectomy or splanchnic nerve section protected three of six animals. This source of catecholamines may not be necessary in every case to initiate the arrhythmia.4. Guanethidine was a relatively ineffective antiarrhythmic agent. Timing appears to be important with this agent.5. Pargyline, by monoamine oxidase inhibition or other mechanisms, significantly lowered the arrhythmic dose of daunomycin. Reserpine pretreatment, on the other hand, prevented any e.c.g. alterations following daunomycin.6. Phenoxybenzamine exerted significant protection which may be related to its cardiodepressant properties.7. Alterations in e.c.g. were seen in all (+)-propranolol pretreated animals, although the arrhythmic period was modified in two of three experiments. Racemic (+/-) propranolol, which exerts direct myocardium depression as well as beta-adrenoceptor blockade, was completely protective in four of five experiments. The results of the present experiments indicate that the sympathetic nervous system is intimately involved in the daunomycin arrhythmia.


Studies on the respiration of Listeria monocytogenes variants with different virulence.


Effects of foveal prestriate and inferotemporal lesions on visual discrimination by rhesus monkeys.


Immunochemical properties of ribonuclease T1 in relation to other ribonucleases.


Characterization of Bacillus subtilis bacteriophage. I. Morphology of phage Vx and properties of its DNA.


Characterization of Bacillus subtilis bacteriophage. II. Isolation and morphology of phage Nf and properties of its DNA.


Cytotoxic factor demonstrated in lymph node extract.


Metabolism of [14C]ethynyloestradiol in the baboon.


Micrococcus luteus deoxyribonucleic acid polymerase. Studies of the enzymic reaction and properties of the deoxyribonucleic acid product.


Platelet acid phosphatase availability.

Platelet acid phosphatase availability.


The menstrual cycle and behaviour of the talapoin monkey (M. talapoin).


On the role of DNA in wet heat sterilization of micro-organisms.


Report on cyclamates by the committee set up by the Israel Ministry of Health.


Rifampicin in the treatment of resistant pulmonary tuberculosis.


Rare ileocecal lesions.

Rare ileocecal lesions.


In vitro susceptibility of Neisseria meningitidis to rifampin.


A comparative structural study on the hemoglobins in Macaca speciosa.


Solubilization, purification, and some properties of trehalase from honeybee (Apis mellifera).


Purification and properties of glycogen phosphorylase from bovine corpus luteum. Kinetics of salt activation.


Cell bound proteolytic enzymes of Arthrobacter.


Requirement of divalent cation for human platelet phosphorylase activity.


Properties of ornithine aminotransferase from rat liver, kidney and small intestine.


Reversible cerebral oedema complicating diabetic ketoacidosis. A young man with diabetic ketoacidosis developed the clinical features of cerebral oedema. This resolved without specific treatment. The mortality from ketoacidosis might be reduced if the syndrome was suspected and treated earlier.


Macroglobulinaemia treated with prednisone, azathioprine, and folic acid.


Prostatic reservoir of gonococci.

Prostatic reservoir of gonococci.


Diabetic ketoacidosis.

Diabetic ketoacidosis.


The effect of drugs on the ballistocardiogram.


The metabolic fate of nitroglycerin (trinitrin) in relation to its vascular effects.


Effects of nitroglycerin (trinitrin) on vascular smooth muscle.


Experimental haemorrhagic hypotension: a study of the influence of dexamethasone in monkeys.


Xanthoma diabeticorum: eruptive xanthoma and diabetes mellitus.


Observations on the intestinal carriage of Pseudomonas aeruginosa.


The influence of certain lipids and proteins on the effect of tetanus toxin in mice.


The fate of gonococci in polymorphonuclear leucocytes.


Lysis and protection of erythrocytes by T-mycoplasmas.


Biochemical genetics of bacterial sporulation. 3. Correlation between morphological and biochemical properties of sporulation mutants.


Callus formation after acute brain damage.


DNA fixation and development of transformability and transfectability in Bacillus subtilis.


Genetic and transfection studies with B, subtilis phage SP 50. I. Phage mutants with restricted growth on B. subtilis strain 168.


Genetic and transfection studies with B. subtilis phage SP50. II. Temperature sensitive mutants and the establishment of a linkage map.


Placental transfer and metabolism of f-14C-cortisol in the pregnant monkey.


Very early replication of scrapie in lymphocytic tissue.


Intravenous hyperalimentation.

Intravenous hyperalimentation.


Albumin losses in bile after partial hepatectomy.


Experimental middle ear transplants. A preliminary report.


Methodology of zinc determinations and the role of zinc in biochemical processes.


Behavioral responses of free-ranging rhesus monkeys to food shortage.


Metabolism of palmitate by fetal, neonatal, and adult muscle of the rhesus monkey.


Mesenteric vascular responses to endotoxin in the monkey and dog.


Sucrose and inulin space measurements of cerebral cortex in four mammalian species.


Methods of virus formation.

Methods of virus formation.


Recent results on the biochemistry of the cell wall lipopolysaccharides of Salmonella bacteria.


Regulation of glutamine synthetase by enzyme catalyzed structural modification.


Nucleosides and nucleotides as potential therapeutic agents.


4-isothiocyanatobenzenesulfonic acid as reagent for Edman peptide degradation.


Biogenesis of steroids in higher plants.


Nickel carbonyl poisoning. Report of 25 cases.


Health services at the crossroads: issues of public policy.


Initial steps in pyrimidine synthesis in Ehrlich ascites carcinoma in vitro. II. The synthesis of carbamyl phosphate by a soluble, glutamine-dependent carbamyl phosphate synthetase.


Combination of bovine carbonic anhydrase with a fluorescent sulfonamide.


Contractile responses at the surface of an amphibian egg.


Australia antigen (a review).

Australia antigen (a review).


Topical antibiotics in war wounds: a re-evaluation.


Bacterial quantification of open wounds.


Initial management of hand wounds.

Initial management of hand wounds.


Blunt trauma and the normal spleen: peacetime experience at a military hospital in Europe.


The use of the liver scan in the treatment of hepatic abscess.


Malignant melanoma: a brief review of current therapy.


Thyroid function in active acromegaly.

Thyroid function in active acromegaly.


Subacute gastric dilatation in air evacuated combat injuries.


Influenza.

Influenza.


Chlorpromazine hydrochloride auto-oxidation: implications for clinical practice.


The influence in male baboons, of a high sucrose diet on the portal and arterial levels of glucose and fructose following a sucrose meal.


Comparison of survival times of monkeys and rats exposed to pulsed and steady-state reactor radiations.


Microcloning and replica plating of mammalian cells. A technique for cloning single mammalian cells has been developed by means of a microculture method (0.010 milliliters per culture). A technique of replica plating has also been developed for use with clones of single cells in microcultures; this technique permits the screening of desired properties of many clones simultaneously before the growth of stock cultures. With these techniques an investigator can with limited budget, space, and personnel perform research using tissue culture systems.


Carbonic anhydrase content of turtle urinary bladder mucosal cells.


Hemolytic anemia with cold detectable IgG antibodies.


Correlation between results of a new T-3 test and the percentage of free thyroxine in serum.


Serum beta-aminopropionaldehyde: identification and origin.


Virus isolations from Ixodid ticks in Uganda. I. Isolation and characterisation of ten strains of a virus not previously described from Eastern Africa.


Influence of antigen receptor density on agglutination of red blood cells.


Further observations on the immunological relationships among serum globulins of man and other primates, revealed by a serological inhibition test.


Mitogenic activity of bacterial fractions in lymphocyte cultures. Endotoxins and other derivatives of gram-negative bacteria.


Immune response to cell wall and tissue antigens in rabbits injured with streptococcal cell wall fragments.


Morphological studies on the peripheral circulation of the genital organs in buffaloes with special reference to spontaneous arteriosclerosis in animals.


The coronary vessels in the Egyptian water buffalo (Bos-Bubalus-bubalis L.).


Influence of a clinical anaesthesia-technique (premedication with tranquillizers and atropine, induction with chloralhydrate, maintenance with halothane in a closed circle system) on liver function tests in the horse.


Allogenic heterotopic canine liver homotransplantation. I. A technique for total hepatectomy.


Characterization of the subunits of swine kidney leucine aminopeptidase.


Effect of Ca2+ on S-100 in long-term incubation.


The pharmacology of prazepam. A new benzodiazepine derivative.


Purification of luteinizing hormone from sheep pituitary glands and evidence for several physicochemically distinguishable active components.


Progress in state and local air pollution control under the Clean Air Act.


Molecular events in the growth inhibition of Bacillus subtilis by D-tyrosine. The transformable strain of Bacillus subtilis strain 168 is extremely susceptible to growth inhibition by d-tyrosine. The molecular events associated with the inhibition of growth by d-tyrosine in this strain include the false feedback inhibition and probably the false repression of prephenate dehydrogenase. These effects were found to contribute to the formation of d-tyrosine-containing proteins by decreasing the intracellular concentration of l-tyrosine. Accordingly, growth inhibition of strain 168 by the d isomer of tyrosine was shown to be progressive, enduring, and delayed by prior growth on l-tyrosine. The synthesis of cellular macromolecules and viable cell count were progressively diminished in d-tyrosine-inhibited cultures. Several different enzyme activities were reduced after growth in the presence of d-tyrosine. Isotopic d-tyrosine was incorporated into cellular proteins without change of optical configuration. Long chains of cells with completed septa were observed microscopically, and therefore some cell wall effect may also be implicated.


Energy generation and utilization in hydrogen bacteria. Studies on the relationship between cell synthesis and energy utilization in Hydrogenomonas eutropha have shown that the amount of oxidative energy required for synthetic reactions depends on the conditions of growth. The energy of hydrogen oxidation was most efficiently used when growth conditions were optimal (continuous culture, cells in exponential growth phase) and when the rate of growth was limited by H(2) or O(2) supply. Under these conditions, 2 to 2.5 atoms of oxygen were consumed by the oxyhydrogen reaction for the concomitant conversion of 1 mole of CO(2) to cell matter. This conversion efficiency, expressed as the O/C energyyield value, was observed with continuous cultures. A less efficient conversion was found with batch cultures. With limiting concentrations of CO(2) the rate of hydrogen oxidation was relatively high, and the O/C value was dependent on the growth rate. With nonlimiting concentrations of CO(2), the rate of hydrogen oxidation was strictly proportional to the rate of CO(2) fixation, and the O/C value was independent of growth rate. This proportionality between the rate of H(2) oxidation and the rate of CO(2) fixation suggested that energy supply regulates the (maximum) rate of growth. From the energy-yield measurements, we concluded that the oxidation of 1 mole of H(2) yields the equivalent of 2 moles of adenosine triphosphate for H. eutropha, and that at least 5 moles of this high-energy phosphate is required for the conversion of 1 mole of CO(2) into cellular constituents.


Characterization of canine mycoplasmas by polyacrylamide gel electrophoresis and immunodiffusion. Canine mycoplasmas which had been characterized by biological and serological methods were further studied by using polyacrylamide gel electrophoresis (PGE) and double diffusion in agar gel. The three dog mycoplasmas previously characterized, Mycoplasma canis, M. maculosum, and M. spumans showed distinctive patterns by PGE. Five additional representative isolates from dogs had been characterized serologically and biologically into three new groups, A, C, and D. An additional mycoplasma (group B) was indistinguishable from M. canis by growth inhibition and PGE but was more broadly reactive with field isolates serologically. The group A organisms were distinctive in pattern and similar to those studied by Razin and Rottem, tentatively designated M. edwardii. The group C organisms were represented by two isolates which were similar by fluorescent-antibody studies but different by growth inhibition tests. These two isolates were also different from each other by PGE. The group D serotypes were also distinctive by PGE from all other dog mycoplasmas tested. It was found, during these studies, that two different mycoplasmas showed different PGE patterns at different intervals during incubation. Immunodiffusion studies showed a relationship among all the canine mycoplasmas, and bands of nonidentity between the two group C mycoplasmas were demonstrated.


Carbon dioxide fixation by cells of Streptococcus faecalis var. liquefaciens. Fixation of NaH(14)CO(3) by a heavy cell suspension of Streptococcus faecalis var. liquefaciens was studied. Several nutrients, pyridoxal, riboflavine, adenine, uracil, and O(2) stimulated (14)CO(2) incorporation into cells only under conditions that were adequate for synthesis of cell macromolecules. Biotin increased CO(2) incorporation in the absence of extensive synthesis of macromolecules, whereas O(2) inhibited incorporation under these conditions. When (14)CO(2) fixation was occurring during synthesis of macromolecules, 71% of the (14)C was incorporated into cells and 29% occurred extracellularly. Ninety-three per cent of the cellular (14)C was in protein and 5.5% was in nucleic acid. Aspartic acid was the only amino acid in the protein fraction that was radioactive. Eighty-three per cent of the extracellular (14)C was resistant to precipitation by trichloroacetic acid. When (14)CO(2) fixation was occurring in cells that were not carrying on extensive synthesis of macromolecules, 38% of the (14)C was incorporated into cells and 59% occurred in the supernatant fluid. Sixty-nine per cent of the cellular (14)C was in protein, 21% was in low-molecular-weight compounds, and 9% was in nucleic acid. Addition of unlabeled aspartate to the medium inhibited incorporation of (14)CO(2). Based on studies of the rate of (14)CO(2) fixation, the cells fix CO(2) into a pool of intermediates which are either used for synthesis, primarily protein, or are excreted into the medium.


Metabolic influences on tyrosine excretion in Bacillus subtilis. The biosynthetic pathway for tyrosine synthesis is regulated both by repression of enzyme synthesis and by feedback inhibition of enzyme activity in Bacillus subtilis. Nevertheless, wild-type cells produce significantly more tyrosine than is required for protein synthesis, and part of this is excreted into the medium. Alteration of nutritional and other environmental conditions of cultivation strongly influenced the amount of tyrosine excretion. The excretion of tyrosine by wild-type cells was compared with that of a regulatory mutant having a feedback-insensitive prephenate dehydrogenase. Tyrosine excretion varied directly with the in vitro activity of prephenate dehydrogenase and inversely with temperature in the two strains. The regulatory mutant excreted five times as much tyrosine as the wild type at all growth temperatures examined. The carbon source used for growth significantly influenced the level of tyrosine excretion. The specific activity of prephenate dehydrogenase was also affected by the carbon source. Incorporation studies with isotopic tyrosine and fluorometric determinations of tyrosine concentrations extractable in hot water were used to measure operationally the tyrosine pools in the mutant and wild-type strains. The effects of various environmental conditions on the synthesis and excretion of tyrosine led to the conclusion that metabolic controls governing end product contrations exist which are completely independent of regulation by feedback inhibition and repression.


Chronic lymphatic leukemia associated with macroglobulinemia.


Rifampicin inhibition of ribonucleic acid and protein synthesis in normal and ethylenediaminetetraacetic acid-treated Escherichia coli. The kinetics of ribonucleic acid (RNA) and protein synthesis in rifampicin-inhibited normal and ethylenediaminetetraacetic acid (EDTA)-treated Escherichia coli was measured. Approximately 200-fold higher external concentrations of rifampicin were needed to produce a level of inhibition in normal cells comparable to that observed in EDTA-treated cells. The rates of RNA and protein synthesis in both kinds of cells decreased exponentially, after an initial lag phase, at all rifampicin concentrations tested. The lag phase was longer and the final exponential slope less for protein synthesis than for RNA synthesis at a given rifampicin concentration. Below certain rifampicin concentrations, both the lag phase and the subsequent exponential decrease in the rates of RNA and protein synthesis were found to be rifampicin concentration dependent. At greater concentrations only the time of the lag phase was decreased by higher rifampicin concentrations, whereas the slope of the exponential decrease in the rates of RNA and protein synthesis was unaffected. In all cases, the exponential decrease continued to at least a 99.8% inhibition of the original rate of synthesis. These in vivo results are consistent with the mode of rifampicin action determined from in vitro studies; rifampicin prevents initiations of RNA polymerase on deoxyribonucleic acid, but not its propagation, by binding the enzyme essentially irreversibly. The results also indicate the size distribution of messenger RNA molecules in E. coli under our conditions.


Animal sources of Salmonella in Israel (1963 to 1967).


Effect of low temperature on the growth and fine structure of Bacillus subtilis. Logarithmically growing cultures of Bacillus subtilis transferred from 37 to 15 C present atypical growth curves, and ultrathin sections of such cells reveal structural modifications involving mesosome deterioration and double cell wall formation. After a time, optical density and viable count increase, and cells regain the appearance typical of control cells, indicating a recovery from thermal stress. Subcultures of such recovered cells continue to grow well at 15 C. Cultures transferred from 37 to 12 C show atypical growth and fine structure, although no recovery from this stress is seen. Cultures previously grown at 15 C continue to grow at 12 C, and, furthermore, do not show the ultrastructural alterations seen in similar cells with a 37 C thermal history. The results of these studies suggest that low temperatures induce structural modifications in B. subtilis, that the response of a population to thermal stress may change during the period of the stress, and that thermal history may influence the response of a population to thermal stress.


Lysis of blue-green algae by myxobacter. Enrichment from local fishponds led to the isolation of a bacterium capable of lysing many species of unicellular and filamentous blue-green algae, as well as certain bacteria. The isolate is an aflagellate, motile rod which moves in a gliding, flexuous manner; the organism is capable of digesting starch and agar, but not cellulose and gelatin. Its deoxyribonucleic acid base pair composition (per cent guanine plus cytosine approximately 70) shows a close resemblance to that of the fruiting myxobacteria. Algae in lawns on agar plates were lysed rapidly by the myxobacter, but only limited and slow lysis occurred in liquid media, and no lysis took place when liquid cultures were shaken. No diffusible lytic factors would be demonstrated. Continuous observation of the lytic process under a phase-contrast microscope suggested that a close contact between the polar tip of the myxobacter and the alga is necessary for lysis. The lytic action is limited to the vegetative cells of the algae, whereas heterocysts are not affected. The gas vacuoles of the algal host are the only remnant visible after completion of digestion by the myxobacter.


Mode of action of 2-nitro-1-butyl pyridinium sulfate. Ten micrograms of 2-nitro-1-butyl pyridinium sulfate (NBPS) per milliliter inhibited the growth of Bacillus subtilis by blocking thymidylate synthetase. The inhibition could be reversed by the addition of thymine or cytosine.


Induction of histidine-degrading enzymes in Pseudomonas aeruginosa. Urocanate but not histidine was able to induce formation of histidine-degrading enzymes in a histidine ammonia-lyase-deficient mutant of Pseudomonas aeruginosa. The results, in conjunction with others reported previously, indicate that urocanate, the first intermediate, is the physiological inducer of the pathway.


Cellular fatty acids of pathogenic Neisseria. The cellular fatty acid composition of 20 isolates of Neisseria gonorrhoeae and 21 isolates of N. meningitidis was examined by gas-liquid chromatography. Each isolate of the two species possessed similar fatty acid profiles which were characterized by five major acids, accounting for 80 to 85% of the total. The three most abundant acids in each species were palmitic, palmitoleic, and beta-hydroxylauric acids; lauric and myristic acids were the next most abundant. The presence of large amounts of beta-hydroxylauric acid (20% or greater) and the relative concentrations of the other four major acids appear to be useful markers for distinguishing N. gonorrhoeae and N. meningitidis fatty acids from those of other bacteria.


Behavior of bovine ephemeral fever virus in laboratory animals and cell cultures.


Evidence for a second haemoglobin alpha-locus duplication in Macaca irus.


Diverticulum of the female urethra.

Diverticulum of the female urethra.


Comprehensive regional approach to the chronic renal failure problem.


Lupus erythematosus and panniculitis.

Lupus erythematosus and panniculitis.


Diabetic ketosis. Interpretation of elevated serum glutamic-oxaloacetic transaminase (SGOT) by multi-channel chemical analysis.


Plasma amino acid levels in diabetic ketoacidosis.


Electron microscopic studies on the mitochondria and intramitochondrial microorganisms of Halteria geleiana.


The antigens of seminal vesicle and seminal plasma.


Deficiency of hepatic organic anion-binding protein, impaired organic anion uptake by liver and "physiologic" jaundice in newborn monkeys.


Y and Z: new terms in the bilirubin equation.


Effects of mucin upon growth of low birth-weight infants and their intestinal microflora.


Clinical, epidemiological and virological studies of aseptic meningitis in Aomori district during seven-year period, 1961-1967, with special reference to ECHO virus infections.


Mumps meningoencephalitis in children.

Mumps meningoencephalitis in children.


Pulp reactions to cavity and crown preparation.


Acid phosphatases from different cell types in rat liver.


Mutants of Pseudomonas aeruginosa strain 1 with unusual acetohydroxy acid synthetase enzymes.


Studies on seed extracts. II. Interaction of erythrocytes of various animal species with seed agglutinins (lectins).


Enzymology in clinical medicine.

Enzymology in clinical medicine.


Studies on Yersinia (Pasteurella) pseudotuberculosis. I. Sources and serological classification of the organism isolated in Japan.


Recognition and management of hypercalcemia. A review.


Removal of fluorescein isothiocyanate from Sephadex after filtration of conjugated proteins.


In utero disease and the newborn infant.


Effect of interfering substances on colorimetric reactions for deoxyribonucleic acid.


Enzyme and bacteriophage concentration by membrane filtration.


Fractionation of competent Bacillus subtilis cells by sucrose density gradient centrifugation.


Derivatives of Bacillus amyloliquefaciens ribonuclease (barnase) isolated after limited digestion by carboxypeptidases A and B.


Novel protein composition of a bacterial membrane.


Wall autolysin of Lactobacillus acidophilus strain 63 AM gasser.


Structure of the walls of Lactobacillus acidophilus strain 63 AM Gasser.


Properties of Dendrostomum pyroides hemerythrin.


Effects of red cell stroma-free hemoglobin solution on renal function in monkeys.


Pulmonary pressure-volume changes attending head injury.


Effect of cycloheximide on production of diamine oxidase by the human placenta.


Effect of anticoagulants on human plasma colloid osmotic pressure measurements.


Sporulation of Bacillus subtilis in continuous culture. Sporulation of Bacillus subtilis 168 was studied in chemostat cultures. Sporulation occurred at high frequency under limitation of growth by glucose or the nitrogen source in minimal medium, whereas rates of sporulation were low for Mg(2+), phosphate, citrate, or tryptophan limitation. Sporulation was found at all growth rates tested, and the incidence of spores increased with decrease in growth rate of the culture. Within the range of growth rates up to the maximum obtainable with the defined medium, no threshold effect of growth rate on sporulation was observed. By studying transient states, it was possible to determine the time taken for the appearance of a refractile spore after initiation of a cell to sporulation. Under conditions of glucose limitation, cells were found to be committed to sporulation as soon as they were initiated. In nitrogen-limited cultures, however, a partial relief of nitrogen limitation prevented the development of spores during the first hour after initiation. The results of experiments with multistep changes in dilution rate of a chemostat culture indicate that initiation to sporulation is probably restricted to a particular point in the cell division cycle.


Isolation and serological characterization of a cell wall antigen of Rothia dentocariosa. A soluble polysaccharide antigen from the cell wall of Rothia dentocariosa ATCC 17931 has been isolated, purified and characterized by serological and chemical procedures. The polysaccharide (RPS) was found to be located at the surface of cells grown under diverse environmental conditions, and could be easily detected on cells in pure culture or in clinical samples from humans and experimentally infected hamsters by fluorescent-antibody techniques. Fructose, glucose, galactose, and ribose were the major constituents of RPS. Although purified RPS was not immunogenic in rabbits, it was presumed to be a major antigen of the cell because it could specifically absorb approximately one-third of the antibody nitrogen in antisera prepared against whole cells of R. dentocariosa. Haptene inhibition studies indicated that fructose was the principal determinant of serological specificity in RPS. This polysaccharide was found to be serologically unique and did not cross-react with the polysaccharides and surface polymers of other oral actinomycetes and filamentous organisms.


Induction of enterococcal L-forms by the action of lysozyme. Suspensions of enterococci were treated with lysozyme in the presence of osmotic stabilizers. The resulting osmotically fragile bodies prepared from Streptococcus faecium strain F24 and S. faecalis strain E1 gave rise to L-forms under optimal osmotic and nutritional conditions for treatment and subsequent growth. The most critical component of the growth medium, to obtain maximum yields, was the nature and concentration of the added salt. The two most effective salts were sodium chloride and ammonium chloride in the range of 2 to 3% (w/v) added to a suitable agar base. Ammonium chloride was more versatile, because it could be used with either sucrose or polyethylene glycol 4000 as the osmotic stabilizer for preparation and dilution of the osmotically fragile bodies. Sodium chloride would not consistently support growth of S. faecium F24 as L-forms when polyethylene glycol 4000 was used as the osmotic stabilizer during lysozyme treatment. Time-course studies of concurrent cell wall removal and L-form induction suggested that maximal induction required only cell wall damage rather than complete wall removal. This method for induction of L-forms from a suspension of enterococci is a significant improvement over other presently known methods.


Fractionation of DNA on a metal ion equilibrated cation exchanger. I. Chromatographic profiles of DNA on an IR-120 A13+ column.


Transduction and genetic homology between Pseudomonas species putida and aeruginosa. Genetic crosses occur by transduction between the species Pseudomonas aeruginosa and P. putida. The frequency relative to intraspecific transfer is reduced and varies among markers, suggesting that these genomes contain discrete regions of homology and nonhomology.


Amnesic syndrome: consolidation or retrieval?


Species specific aggregation of dissociated sponge cells.


One-dimensional pattern found in blue-green algae.


An introduction to electron beam therapy.


Retinal ganglion cells: a correlation of anatomical and physiological approaches.


The electroretinogram: its components and their origins.


The role of sialic acid in hemagglutination.


Immunochemical characteristics of rat skeletal muscle myosin.


Importance of the generation time in microbiological experiments.


Regulation of formation of proteases in Bacillus megaterium. IV. The formation of the enzyme in the sporogenous strain KM.


Clinical pharmacy: long-term care institution.


Cell mediated immunity in parasitic infections.


Effect of anticoagulants on the transplanted heart.


Assessing the balance of physician manpower in a metropolitan area.


Subunit heterogeneity in human serum beta lipoprotein. The apoprotein of human serum beta lipoprotein has been prepared in a lipid-free and soluble form by a technique of interfacial extraction in the presence of guanidinium hydrochloride and nonionic amphiphile. Gel permeation chromatography of maleylated apobeta lipoprotein separates two protein fractions with different amino acid compositions. The smaller particle has a molecular weight of 2.6 x 10(4).


The legal scope of dental hygienists in the United States and other countries.


Anticipated health behavior of families in relation to Medicaid.


Analysis of the diphtheria outbreak in Austin, Texas, 1967-69.


Use of the Chandrasekar-Deming technique in the Liberian fertility survey.


Coronary risk factors of male workers on a Kauai, Hawaii, plantation.


Childhood characteristics as indicators of adult health status.


Followup of mael and female contacts of patients with gonorrhea.


Intramural diverticulosis of the esophagus.


Histopathology of late local radiolesions in the goat brain.


Instillation of thio-tepa (Tifosyl) in vesical papillomatosis.


Effects of bioisolation.

Effects of bioisolation.


The influence of amphotericin B upon Histoplasma infection in dogs.


Light and electron microscope study of chlorobiphenyl poisoning. In mouse and monkey liver.


Isolation and propagation of a virus from a spontaneous mammary carcinoma of a rhesus monkey.


Marihuana and the diabetic coma.

Marihuana and the diabetic coma.


Precipitating antibodies to DNA induced by heat-denatured DNA-albumin conjugates in the rabbit.


Isolation and genetic analysis of temperature-sensitive mutants of B. subtilis defective in DNA synthesis.


Rifampin. Levels in serum and saliva and effect on the meningococcal carrier state.


Exercise and cholesterol catabolism.

Exercise and cholesterol catabolism.


A potential method for selecting genetic deletions in Escherichia coli.


Effect of ionic calcium and magnesium on human platelet aggregation.


Bicarbonate secretion by the rat colon: effect of intraluminal chloride and acetazolamide.


Application of immunodiffusion methods for typing members of the Phlebotomus group of arboviruses.


Studies of replication of four selected viruses in two cell lines derived from salmonid fish.


Morphine and ethanol physical dependence: a critique of a hypothesis.


Ascorbic acid depletion in patients undergoing chronic hemodialysis.


Experimental studies of toxic factors in uremic encephalopathy.


The Blochmann bodies: hereditary intracellular symbionts of insects.


Rubber men.

Rubber men.


The oblique facial cleft. A report of 2 cases and a review of 41 cases.


Intrathecal chemotherapy. Selection of cytostatic agents. Selection of cytostatic agents for intrathecal administration is the subject of this paper.Both the toxic side effects-destruction of blood-brain barrier and change of body weight-and the cytostatic effects on intracranially transplanted Yoshida ascites sarcoma were investigated of intrathecal administration of various cytostatic agents. As a result, it may be concluded that Methotrexate and Endoxan and lower dose of mitomycin C are suitable drugs for intrathecal chemotherapy.Based on these findings, clinical cases of malignant brain tumours were treated with intrathecal chemotherapy.Grateful acknowledgement is made to Professor Dennosuke Jinnai for his constant interest and guidance in this investigation.


Health Services financing report: a review of appendices A-Q. In May of this year after three years' work an advisory panel of the B.M.A., with Dr. I. M. Jones in the chair, produced a report on Health Services Financing.(1) A summary appeared in the Annual Report of Council(2) and a preliminary discussion on the report was held at the B.M.A.'s Annual Representative Meeting in July.(3) A full debate was deferred until the committees, divisions, and branches of the Association had had an opportunity to discuss the proposals. Nearly half of the book consists of appendices, commissioned from several economists, which cover the N.H.S. and the health services of 14 Western and some Eastern European countries. Below Professor Dennis Lees reviews these appendices, which formed a major source of information for the authors of the report and therefore have an important bearing on their conclusions.


The early clinical diagnosis of tuberculosis in baboons.


Comparative biochemistry of the primates. 3. Imidazole metabolites in the blood and urine.


Delayed response deficits in neonatally asphyxiated rhesus monkeys.


Disruptions of auditory sequence discrimination by unilateral and bilateral cortical ablations of superior temporal gyrus in the monkey.


Effect of harmaline in monkeys with central nervous system lesions.


Infections of Plasmodium vivax in Saguinus geoffroyi.


Action of N-methyl-N'-nitro-N-nitrosoguanidine on transforming DNA of Bacillus subtilis.


Enzyme purification by selective elution with substrate analog from ion-exchange columns: application to glucose 6-phosphate dehydrogenase, pseudocholinesterase, lactate dehydrogenase, and alanine dehydrogenase.


Rheumatic fever chemoprophylaxis and penicillin-resistant gingival organisms.


Drug-resistant pulmonary tuberculosis treated with ethambutol, a rifamycin and a riminophenazine (B663).


Immunosuppression with cell membrane extracts.


Observations on the decline in immunosuppressive potency of rabbit anti-mouse thymocyte sera raised by repeated immunization of the same rabbit.


Long acting phenothiazines and long term psychotic patients: the role of fluphenazine enanthate in treatment.


Fluphenazine enanthate given to inpatients and outpatients.


Comparative study of gonococcal susceptibility to penicillin in the United States, 1955-1969.


Man, milieu and malady. What do we mean by the structure of behavior.


The American Urological Association and medical care crisis.


The doctor and the government.

The doctor and the government.


Practical serology of the systemic mycoses.


Experience with arteriovenous fistula in chronic hemodialysis.


Neurotoxic reaction to penicillin and carbenicillin.


Partial plaster splint for the sprained ankle.


Demethylchlortetracycline in the management of urologic infections.


Comparison of the combination therapy of capreomycin. Ethionamide. Cycloserine with that of kanamycin. Ethionamide. Cycloserin in the retreatment of pulmonary tuberculosis.


Elevated histaminase activity in medullary carcinoma of the thyroid gland.


The effect of 10 weeks consumption of a 75 percent sucrose diet on the incorporation of [U-14C] fructose into the serum glycerides of male and female baboons.


Marginal plate in human hepatic microbody.


Histaminase and medullary thyroid carcinoma.


Sports and athletic injuries: a physiotherapist's approach.


Studies on the downer syndrome in dairy cows.


Preparation of antisera and identification of blood groups in dogs.


Location of retrograde changes in the nervous system following unilateral lympathectomy in sheep.


The biometry of the gravid uterus in Egyptian buffalo cows.


Recklinghausen's disease: a multifacetted syndrome.


Ethylene oxide resistance of micro-organisms in dust compared with the resistance of Bacillus subtilis spores.


Endogenous incorporation of 32P in Neisseria meningitidis. 2. The effects of acetate, succinate, propionate and acetoacetate.


Proteolytic enzymes and biological inhibitors. I. Comparison between the Kunitz method and the agar gel casein precipitating reaction for determination of the activity of some commercial proteolytic enzymes and inhibitors.


Rheumatic tissues in antibiotic-free tissue culture.


Subcorneal pustular dermatosis. Pathogenetic aspects.


Oxytocin-induced water intoxication?

Oxytocin-induced water intoxication?


Oculo-palpebral lesions following high-speed electron treatment of maxillary sinus tumours.


A seven year survey of changing treatment in carcinoma of tongue.


Experience in a newly-established diagnostic virology laboratory: the first year.


A three-year laboratory survey of bacteriologically confirmed mycobacterial infections.


Experimental pyelonephritis.

Experimental pyelonephritis.


A comparison of the penicillinases from a methicillin-sensitive and froma a methicillin-resistant strain of Staphylococcus aureus.


Factors affecting the radiation resistance of dried bacterial spores.


Various molecular weights for the heavy chains of human monoclonal IgM immunoglobulins.


Predominance of a single mu chain subclass in cold agglutinin heavy chains.


Immunological and immuno-fluorescent studies on keratin of the hair follicle.