Microbiological aspects of ethylene oxide sterilization. IV. Influence of thickness of polyethylene film on the sporicidal activity of ethylene oxide. Spores of Bacillus subtilis var. niger, dried on nonhygroscopic and hygroscopic surfaces, were enclosed in one of four thicknesses of low-density polyethylene film (2, 4, 6, and 20 mils). The surfaces were then placed in a specially designed thermochemical death rate apparatus and exposed to an ethylene oxide concentration of 600 mg/liter (at 54.4 C) and 50% relative humidity. Survival data, including both spore survivor curves and decimal reduction values (expressed as D values at 54.4 C-600 mg of ethylene oxide per liter), demonstrated similar survivor patterns when the B. subtilis var. niger spores were enclosed in low-density polyethylene films 2, 4, and 6 mils thick. A comparison of these patterns with those of spores enclosed in glassine and subjected to identical exposure conditions revealed only slight variations. The use of 20-mil polyethylene film greatly increased the time required to kill B. subtilis var. niger spores under the exposure conditions.


Occurrence of the ciliate protozoa Bütschlia parva Schuberg in the rumen of the ovine. The holotrich ciliate protozoa, Bütschlia parva Schuberg, has been observed in the rumen of the ovine. Limited data suggest that the concentration of B. parva in the rumen follows a diurnal cycle similar to that of the other holotrichs.


Flow chart for safety testing monkey kidney cell suspensions used in vaccine production. A flow chart is presented depicting procedures employed for the detection of bacteria and adventitious agents in monkey kidney cell suspensions used for vaccine production.


Acid-precipitated M protein compared with a column-eluted M protein preparation from type 12, group A Streptococci. An M protein preparation of group A streptococci, precipitated with 0.03 m sodium acetate buffer (pH 4.0) was compared with a column-eluted M protein preparation. Absorption spectra and methyl pentose content were similar in both preparations. Acrylamide gel electrophoresis patterns were different. Gel diffusion demonstrated two lines of fusion in the preparations. More antigens could be demonstrated in both preparations by using immunoelectrophoresis. Neither the pH 4 precipitate nor the column-eluted preparation appeared to be a pure M protein preparation.


1-hydroxy-3-amino-pyrrolidone-2 (HA-966). I. Behaviour and motor effects.


1-hydroxy-3-amino-pyrrolidone-2 (HA-966). II. Extrapyramidal aspects.


Kinetics of distribution and metabolism of diazepam in animals and humans.


Ciliate protozoa in the rumen of the lactating cow.


Chloroplast structure and function in ac-20, a mutant strain of Chlamydomonas reinhardi. I. CO2 fixation and ribulose-1,5-diphosphate carboxylase synthesis. A mutant strain of the green alga Chlamydomonas reinhardi, ac-20, is described in which both the rate of CO(2) fixation by whole cells and the rate of carboxylation of ribulose-1,5-diphosphate in cell-free extracts are reduced, particularly when sodium acetate is present in the growth medium. Of the enzymes of the reductive pentose phosphate cycle tested, only ribulose-1,5-diphosphate carboxylase activity is reduced in the mutant strain, and it appears that the low carboxylase activity limits the strain's rate of photosynthetic carbon metabolism. Evidence is presented to show that the fluctuation in the level of the enzyme activity in the presence or absence of acetate results from the fluctuation in the level of some factor(s) limiting the rate of synthesis of the protein.


Inhibition of gram-positive bacteria by Mucor pusillus.


A temperature-sensitive induction of pyocin R synthesis in a mutant strain of Pseudomonas aeruginosa R.


Gaschromatographic analysis of free myo- and scyllo-inositols in animal tissues.


The infection of Boophilus microplus with Babesia bigemina.


The development of Theileria parva in the salivary glands of the tick, Rhipicephalus appendiculatus.


Genetic control of immunological responsiveness in guinea pigs to 2,4-dinitrophenyl conjugates of poly-L-arginine, protamine, and poly-L-ornithine. The ability of guinea pigs to respond immunologically to dinitrophenyl (DNP)-poly-L-arginine, DNP-protamine, DNP polyornithine, and to unsubstituted poly-L-arginine and protamine is linked to the presence of the poly-L-lysine gene. This gene has previously been demonstrated to control the immune response of guinea pigs to poly-L-lysine, to a copolymer of L-glutamic acid and L-lysine and to haptenic derivatives of these materials. Although the ability to respond to each of these highly charged compounds is linked to the presence of the poly-L-lysine gene, the cellular immune responses to these antigens is highly individually specific.


Studies of the aromatic circular dichroism of Staphylococcal nuclease. Specific contributions of tyrosyl and of tryptophanyl residues can be distinguished in the near-ultraviolet circular dichroic spectrum of staphylococcal nuclease. Upon binding of the inhibitor deoxythymidine 3',5'-diphosphate in the presence of Ca(++), a significant change in the circular dichroic spectrum results which has been used to characterize the interaction of ligand and enzyme. The data suggest that the asymmetric environment of certain tyrosyl residues is altered by binding of the nucleotide inhibitor.


Histochemical study of carbonic anhydrase in the kidney of mouse, with special reference to the inhibitory effect of diamox.


Methods for differential diagnosis of consumption coagulopathy.


The effect of three alkylating agents on the seminiferous epithelium of rodents. I. Depletory effect.


The effect of three alkylating agents on the seminiferous epithelium of rodents. II. Cytotoxic effect.


The muscle in diabetes mellitus. A histologic (light and electron microscope) and biochemical study by means of needle biopsy.


The maintenance of human synovial tissue in vitro.


Studies on the effect of denervation in developing muscle. I. Differentiation of the sarcotubular system.


Scanning electron microscopic studies on the long term effects of sodium glutamate on the rat retina.


Scanning electron microscopy of the retina in vitamin A-deficient rats.


Sensitization to house dust, bacteria and fungi in byssinosis.


Modifications with chelating agents of the effects of gamma-ray fractionated exposures on chromosome aberrations.


Presence of human reagin in immunochemically pure IgG serum fractions.


Skin-sensitizing antibodies in serum from patients with penicillin allergy studied by passive transfer to monkey skin and compared with results obtained on human skin (Prausnitz-Küstner technique).


Rifampicin-susceptibility and rifampicin-resistance in mycobacteria.


Sensitivity test of M. tuberculosis against rifampicin: a short report.


The antimycobacterial activity of rifampicin "in vitro".


Mechanism of rifampicin action on mycobacterial cells. Consideration to the potentiation problem of antimycobacterial treatment.


Rifampicin activity "in vitro" and in established tuberculosis in mice.


The "in vitro" activity of rifampicin compared with ethambutol, capreomycin and isoniazid.


Hematologic and biochemical studies in a case of lead poisoning.


Changing etiology of bacterial endocarditis in the antibacterial era. Experiences at Boston City Hospital 1933-1965.


Pulmonary allergic aspergillosis.

Pulmonary allergic aspergillosis.


The fine structure of Leucocytozoon simondi. I. Gametocytogenesis.


Tyrosinase inhibitors in amelanotic and melanotic malignant melanoma.


Thermophilic blue-green algae and the thermal environment.


Evaluation of serum enzyme studies with special reference to serum histaminase in early diagnosis of acute myocardial infarction.


Serological and skin test cross-reactivity between Histoplasma capsulatum and Oidiodendron kalrai.


Further studies of the keratinophilic fungi in African soils.


The specific property of paraprotein lipids in serum concerning the heparin clearing effect.


Intestinal flora of folate-deficient mice.


The macronuclear envelope of Tetrahymena pyriformis GL in different physiological states. II. Frequency of central granules in the pores.


The diagnostic value of lysozyme (muramidase) estimation in biological fluids.


Susceptibility of group D streptococcus (enterococcus) to 21 antibiotics in vitro, with special reference to species differences.


The fluoric origin of the bone lesion in the Pithecanthropus erectus femur.


Rapidity of the hypotensive effect of short-acting nitrous compounds.


The Seminole Indians of Florida: morphology and serology.


Endoscopic surgery of the pharyngo-esophageal segment.


Distribution and ultrastructure of the Boettcher's cells in mammals.


Effect of streptomycin on the phosphorus metabolism of Bacillus subtilis.


Antigenic relationships of various fractions of the Ehrlich ascites tumor investigated by different immunoserological methods.


The serological activities of immunoconglutinin.


Tentative method of analysis for sulfur dioxide content of the atmosphere (colorimetric). 42401-0169T.


The sense of flutter-vibration evoked by stimulation of the hairy skin of primates: comparison of human sensory capacity with the responses of mechanoreceptive afferents innervating the hairy skin of monkeys.


Relationships between antibodies and experimental allergic encephalomyelitis. V. Antibodies and delayed hypersensitivity in production and prevention of experimental allergic encephalomyelitis.


A unique rapid enhancement by adjuvant of delayed hypersensitivity to protein antigens in the guinea pig.


Pseudomalignant osseous tumour of soft tissue. A clinical, radiological and pathological study of five cases.


Total dislocation of the cuboid bone. Report of a case.


Development of a closed system for the sustained intravenous infusion of a parenteral solution of 137m Ba-EDTA.


On the application of immunological techniques in geographic group studies of Atlantic sea herring, Clupea harengus. II. Herring serum analysis by gel diffusion.


Silent cholinesterase gene: variations in the properties of serum enzyme in apparent homozygotes. The cholinesterase activity of the sera of 25 subjects diagnosed as homozygotes for the silent cholinesterase gene was studied by a sensitive enzymatic method employing several thiocholine esters and various inhibitors, and by disc electrophoretic, immunochemical, and chromatographic methods.(a) With one exception, the sera fell into two classes by all criteria. One class (type I, 16 cases) had no normal serum cholinesterase. The other class (type II, eight cases) had about 2% of apparently normal serum cholinesterase. The remaining serum was intermediate between the two classes in several respects. One explanation for these results is that there are several "silent" genes concerned; possibly these are allelic.(b) Normal sera and all silent sera contain small amounts of a cholinesterase activity labeled the residual cholinesterase. The enzyme(s) responsible has properties similar to those of acetylcholinesterase rather than serum cholinesterase. It is estimated that about 1% of the activity of normal serum against acetylthiocholine is due to this enzyme. The source of the residual cholinesterase is not yet known.


Reversible effects of antimitotic agents on cortical morphogenesis in the marine ciliate Condylostoma magnum.


An immunochemical study of the surfaces of sponge cells.


Photoreactivation of U.V. reactivation in protozoa.


Methyldopa-chlorothiazide--a long-term evaluation.


Many-termed relations in biology.

Many-termed relations in biology.


The autocatalytic growth model. I. Critical analysis of the conceptual framework.


Ecology as a teleological science.

Ecology as a teleological science.


Spreading of risk and stabilization of animal numbers.


A theoretical analysis of the functional matrix.


Some methodology problems in a holistic approach to functional morphology.


The relationship between form and function and its influence on ontogenesis and phylogenesis.


Pre-biological evolution.

Pre-biological evolution.


A model of pre-programmed differentiation of the larval head region in Limnaea stagnalis.


On the sex in Bonellia viridis.

On the sex in Bonellia viridis.


Short biography of Professor Dr. C. J. van der Klaauw.


The philosophy of nature in Alexander von Humboldt's "Views of Nature". 3 lectures delivered during February of 1967 at the "History of Science-Collection" of the University of Texas.


Aedes simpsoni in Chaggaland, Tanzania.

Aedes simpsoni in Chaggaland, Tanzania.


Diamine oxidase (histaminase) in human pregnancy. The activity of the enzyme in lymph and in blood plasma and the effect of heparin on the latter.


Observations on the life-cycle of Echinococcus oligarthrus (Diesing, 1863) in the Republic of Panama.


The behavior of slender and stout forms of Trypanosoma cruzi in the blood-stream of normal and immune mice.


Vaporized formaldehyde treatment of a textile mill contaminated with Bacillus anthracis.


Thrombosis and blood coagulation. A study by quantitative N-terminal amino acid analysis.


A critical assessment of the conventional Widal test in the diagnosis of tyhoid and parathyphoid fevers.


Studies on antigenicity of six Kyasanur Forest disease virus strains isolated from various sources.


Contagious bovine pleuropneumonia: Serologic response of cattle after single and double vaccination with T-1 culture vaccine.


The primate larynx. A comparative physiological study of intrinsic muscles.


Subcellular distribution of 3H-noradrenaline in adrenergic nerves of mouse atrium--effect of reserpine, monoamine oxidase and tyrosine hydroxylase inhibition.


Tuberculosis--problems in diagnosis and eradication.


An improved single-disk method for testing the antibiotic susceptibility of rapidly-growing pathogens.


Asbestosis and neoplastic disorders of the hematopoietic system.


Attachment of reticulocyte ribosomes to erythroid cell membranes in vitro.


Some spectral and kinetic properties of the acyl enzymes of native and chemically modified subtilisin type Carlsberg and type Novo.


Some characteristics of the binding of substrates of glutamine synthetase from Escherichia coli.


Methods of dating skeletal remains.

Methods of dating skeletal remains.


Inhibition of the amino acid induced initiation of germination of bacterial spores by chlorocresol.


Tumor antigens. 3. Immunological studies of tissue and tumor antigens and serum protein production by transplantable murine tumors.


Agglutinating activity of phytohemagglutinin on peripheral blood cells from chickens.


Gangrenous cholecystitis in a patient with Waldenström's macroglobulinemia.


Restoring the health of digestive fistula patients.


An analysis of health expenditure.

An analysis of health expenditure.


The second branchial arch: rheumatological manifestations.


Regulation of chemoautotrophic metabolism. II. Competition between amino acids for incorporation into Thiobacillus.


Photoreduction of 2,6-dichlorophenolindophenol by chloroplasts with exogenous Mn2+ as electron donor.


Purification and characterization of a proteinase in the venom of Trimeresurus flavoviridis. Complete separation of the enzyme from hemorrhagic activity.


Problems in purification of a Bacillus subtilis autolytic enzyme caused by association with teichoic acid.


Iodine sensitivity of Bacillus cereus penicillinase.


Effect of metal and hydrogen ions on the activity and stability of allantoicase.


Studies on the structure of human haptoglobins. I. Spontaneous refolding after extensive reduction and dissociation.


Porphyrin biosynthesis. VII. Porphyrinogen carboxy-lyase from avian erythrocytes. Purification and properties.


Some immunochemical properties of succinylated human transferrin and related proteins.


Hydrolysis of disulfide bonds in weakly alkaline media. II. Bovine serum albumin dimer.


Pronase and "immunogenic RNA". Inactivation of mouse anti-phage antibody with pronase.


Relevance of diseases in animals.

Relevance of diseases in animals.


Notes on the ultrastructure and sporoblast development in fish parasitizing myxosporidian of the genus Sphaeromyxa.


Viral hemorrhagic encephalopathy of rats. I. Isolation, identification, and properties of the HER strains of rat virus.


Immunologic studies with yellow fever and selected African group B arboviruses in rhesus and vervet monkeys.


Antimalarial activity of 2,4-diamino-6-[(3,4-dichlorobenzyl) nitros-amino] quinazoline (CI-679 base) and CI-679 acetate. Laboratory studies in mice and rhesus monkeys.


Kuru: pathogenesis and characterization of virus.


The activities of chlorinated lincomycin derivatives against infections with Plasmodium cynomolgi in Macaca mulatta.


Paracoccidioidomycosis, (South American blastomycosis). A study of 39 cases observed in Medellin, Colombia.


Immunoglobulin G subclass of human antinuclear antibodies.


Histuria and fibrinuria in cases of systemic lupus erythematosus.


Long-term care; floor meetings.

Long-term care; floor meetings.


Definition of staphylococcal protein A reactivity for human immunoglobulin G fragments.


The distribution of specificity in rabbit antisera directed toward human hemoglobins.


Evolutionary dissociation of allotypes and other antigenic determinants of immunoglobulins in non-human primates.


The effect of copper on the radiosensitivity of bacteria.


Studies with polysomes obtained from rabbits during the immune response to ragweed antigen.


Allergic periodontal disease in sensitized squirrel monkeys.


Determination of Pseudomonas aeruginosa by biochemical test methods. I. An improved method for gluconate oxidation test.


Production of human virus vaccines in primary mammalian cells.


The choice of the cell substrate for human virus vaccine production.


Nalidixic acid cetrimide agar. A new selective plating medium for the selective isolation of Pseudomonas aeruginosa.


Interaction between papova-like virus and paramyxovirus in human brain cells: a hypothesis.


Sensitivity of Neisseria gonorrhoeae to penicillin and other drugs.


Listeria monocytogenes meningitis in adults: a report of two cases.


Polarization effects of sinusoidal 50-cycle alternating current on membrane potential of mammalian cardiac fibres.


Immunogenic studies on non-agglutinable vibrios with particular reference to toxigenicity.


Excessive DNA synthesis in Lactobacillus acidophilus during amino acid starvation.


The generation of chemotactic activity in sera of germ-free newborn piglets by interaction with rough strain of Escherichia coli.


Neutralization of bacterial viruses by antibodies of young animals. II. Studies on the cofactor activity of complement from precolostral piglet serum: comparison with the haemolytic and bactericidal activity.


Immunoconglutinins in human saliva--a group of unusual IgA antibodies.


Mode of action of siomycin.

Mode of action of siomycin.


The enzymatic synthesis of fatty acid methyl esters by carboxyl group alkylation.


The presence of zinc in human cytocuprein and some properties of the apoprotein.


The structure and enzyme-coenzyme relationship of supernatant aspartate transaminase after dye sensitized photooxidation.


Isolation and characterization of alpha 1-acid glycoprotein from chimpanzee plasma.


Specificity of rabbit antibody to formaldehyde treated rabbit serum albumin.


Intravaginal radiation therapy with electrons.


The squirrel monkey and carbohydrate metabolism.


Cervical carcinoma: relationship between histology and survival following radiation therapy.


Studies on chemical inhibition of the rhesus antigen-antibody reaction.


Management of effusions.

Management of effusions.


Chemotherapy and radiation therapy together to treat cancer.


The present place of radiation therapy in the management of esophageal cancer.


Protein abnormalities in the cold haemagglutinin syndrome.


Toxic effects of high concentrations of bromobenzylnitrile vapor in various animal species.


Studies on the binding of selenium by liver homogenates from rats fed diets containing either casein or casein plus linseed oil meal.


Changes in nerve terminals and acini of the lacrimal gland and changes in secretion induced by autonomic denervation.


A clinical study of the autoanalyzer method for prenatal antibody evaluation and screening.


Cellular blue nevus. Melanogenic activity and malignant transformation.


Relation of the hydrophobicity index to the thermal stability of homologous proteins.


Solutions for intravenous feeding.

Solutions for intravenous feeding.


Metastatic carcinoma of the male breast treated with bilateral adrenalectomy and chemotherapy.


Immunologic studies in Burkitt's lymphoma.


Multiplicity of encephalitogenic myelin basic proteins. II. Antigenic determinants related to species of origin and molecular size.


Significance of the quantitative relationships of antigen and adjuvant for the efficiency of the adjuvant.


Immunogenicity of bovine encephalitogenic protein.


Studies on thermophilic alpha-amylase from Bacillus stearothermophilus. II. Thermal stability of thermophilic alpha-amylase.


Studies of the effects of heparin, Coumadin, and vitamin K on prothrombin metabolism and distribution in calves with the use of iodine-125-prothrombin. Characterization of the prothrombin system.


An electrophoretic study of the sol phase of sputum.


Comparison of cephaloglycin and penicillin in streptococcal pharyngitis.


Hidden gastric autoantibodies to intrinsic factor in pernicious anemia.


Cerebral hemodynamics and metabolism following experimental head injury.


Functional localization in the trigeminal ganglion in the monkey.


Dystrophic muscle calcification following cerebral damage; report of three cases.


Respiration and the cerebrospinal fluid in experimental cerebral concussion.


The metabolism of C14-ICI 54,450 (myalex) in various species--an in vivo NIH shift.


Development of tolerance to and physical dependence on barbiturates in rhesus monkeys.


Sites of action of SKF 525-A in nerve and muscle.


Hemorrhagic bullae in gonococcemia.

Hemorrhagic bullae in gonococcemia.


Deformations of the temporal bone in head trauma.


Carcinoma of the cervix treated by external irradiation alone.


Treatment of Ewing's sarcoma with local irradiation and systemic chemotherapy. A progress report.


A serum agglutinin inhibited by ionized calcium.


The investigation of steroid metabolism in early infancy.


The clinical chemistry of bromsulfophthalein and other cholephilic dyes.


Recent advances in the biochemistry of thyroid regulation.


Energy production in anaerobic organisms.


Plant bioregulators in the metathoracic glands of myrmicine ants.


Internal and external contributions to the biogenesis of mitochondrial proteins.


Radioactive drugs.

Radioactive drugs.


Intravenous hyperosmolar alimentation.

Intravenous hyperosmolar alimentation.


Evaluation of adjunctive chemotherapy for recurrent cancer.


Effect of basic cupric acetate on the biochemical changes in the liver of the rat fed carcinogenic aminoazo dye. I. Changes in the activities of DAB metabolism by liver homogenate.


Prostaglandin E2 like activity in monkey lung.


Release of lipopolysaccharide in Escherichia coli resistant to the permeability increase induced by ethylenediaminetetraacetate.


Biochemical studies of bacterial sporulation and germination. 18. Free amino acids in spores.


Biochemical studies of bacterial sporulation and germination. XIX. Phosphate metabolism during sporulation.


Biochemical studies of bacterial sporulation and germination. XX. Phosphate metabolism during germination.


Immunological activity of cytochrome c. II. Localization of a major antigenic determinant of human cytochrome c.


Participation of xanthine oxidase in the activation of liver tryptophan pyrrolase.


Neutralization of residual infectivity of Venezuelan equine encephalomyelitis virus by anti-gamma globulin.


Rocky Mountain spotted fever. Laboratory infection in a vaccinated individual.


An attenuated rubella virus vaccine (Cendehill 51 strain) grown in primary rabbit kidney cells.


Immunization against mumps.

Immunization against mumps.


Summary of classification of animal viruses, 1969.


Implications of infectious nucleic acids in disease.


Prospects for a rubella virus vaccine.

Prospects for a rubella virus vaccine.


A high frequency of cold agglutinins of anti-IA specificity in a New Guinea highland population.


A new factor of the Ag-system: Ag(g).

A new factor of the Ag-system: Ag(g).


An example of anti-Coa found in pregnancy.


Accidental decompression: a new philosophy for the transports of the 1970's.


Ag(d), a further factor of the Ag-system.


Aeromedical consultation service case report: post-traumatic epilepsy.


Gastrostomy: a review of its uses in a general surgical unit.


Pectate lyase and polygalacturonate lyase activity in a Vi antigen-degrading enzyme preparation.


Relative labilities of interchain disulfide bonds in human immunoglobulin M subunits.


Purification and characterization of a lectin (plant hemagglutinin) with blood group A specificity from Dolichos biflorus.


Structure of the peptidoglycan in Escherichia coli B and Bacillus megaterium KM. Stereospecific synthesis of two meso-diaminopimelic acid peptides with the tetrapeptide subunit of bacterial cell wall peptidoglycan.


Latent o-diphenol oxidase in mushrooms (Agaricus bisporus).


Studies on carbohydrate metabolism in fruit tissues of Citrus acida. 3. Ascorbic acid metabolism in fruit tissues of Citrus acida at different stages of the development of the fruit.


Bromal, the product of reaction of aspartic acid with N-bromosuccinimide.


Slide test for detection of slime formation in vibrios.


Effect of an azo dye on germination and outgrowth of Bacillus megaterium spores.


Pathogenesis of oncogenic simian adenoviruses. VII. The origin of annulate lamellae in LLC-MK2 cells infected with SV30.


Acid-fast bacilli in sputum in North Queensland: an analysis pertaining to the years 1967 and 1968.


Crohn's disease of the colon and diverticulitis.


Toxoplasmosis. Report of a WHO meeting of investigators.


WHO Expert Committee on Drug Dependence. Seventeenth report.


African trypanosomiasis. Report of a Joint FAO-WHO Expert Committee.


Gel precipitation reactions between alkaline extracted rubella antigens and human sera.


Combined use of fluorescent antibody technique and culture on selective medium for the identification of Neisseria gonorrhoeae.


Immunodiffusion studies on Escherichia coli. 1. Identification of O, K and H antigens in an O6 strain.


A pharmacological analysis of aspirin.

A pharmacological analysis of aspirin.


Opportunistic infection: a review.

Opportunistic infection: a review.


Trypanosoma cruzi infections in CFI mice. 3. The capability of flagellates obtained from two sources (bug faeces and mouse blood) to induce patent infection on perconjunctival instillation.


The effects of x-irradiation and antibiotics on the susceptibility of Aedes togoi and A. aegypti to subperiodic Brugia malayi and B. pahangi.


Resistance of Pseudomonas to quaternary ammonium compounds. I. Growth in benzalkonium chloride solution. Resistant cells of Pseudomonas aeruginosa and a waterborne Pseudomonas sp. (strain Z-R) were able to multiply in nitrogen-free minimal salts solution containing various concentrations of commercially prepared, ammonium acetate-buffered benzalkonium chloride (CBC), a potent antimicrobial agent. As the CBC concentration increased, growth increased until a point was reached at which the extent of growth leveled off or was completely depressed. Minimal salts solutions of pure benzalkonium chloride (PBC) containing no ammonium acetate did not support bacterial growth. When ammonium acetate was added to PBC solutions in the same concentrations found in CBC solutions, growth patterns developed that were comparable to those found with CBC. Likewise, (NH(4))(2)SO(4) added to PBC solutions supported growth of both organisms. P. aeruginosa was initially resistant to CBC levels of 0.02% and it was adapted to tolerate levels as high as 0.36%. Strain Z-R was naturally resistant to 0.4% CBC. Since ammonium acetate, carried over by the CBC used in drug formulations and disinfectant solutions, has the potential to support the growth of resistant bacteria and thus make possible the risk of serious infection, it is suggested that regulations allowing the presence of ammonium acetate in CBC solution be reconsidered.


In vitro susceptibility of Pseudomonas aeruginosa to carbenicillin and the combination of carbenicillin and gentamicin. One hundred and eleven strains of Pseudomonas aeruginosa isolated from clinical material were studied for susceptibility to carbenicillin. Of the strains, 80% were inhibited by 125 mug/ml or more. The combination of carbenicillin and gentamicin was shown to have inhibitory and bactericidal synergistic effect on 15 of 16 strains of P. aeruginosa tested. There was poor correlation between the single-disc sensitivity method and the tube dilution method.


Reactivity of two selected antigens of Neisseria gonorrhoeae. Two antigen preparations, the soluble antigen and a fraction 1 thereof, isolated in the course of a systematic study of the various antigens of the virulent gonococcus, have been investigated for their ability to serve as antigens for the detection of antibody in patients infected with the gonococcus. The soluble antigen was reactive with 88.2% of the sera from infected females, and fraction 1 was reactive with 71.6% of the sera. Of sera from infected males, only 27.6% reacted with the soluble antigen and only 20.4% with fraction 1. Of sera from individuals presumed free of gonococcal infection, approximately 4% reacted with the soluble antigen; none reacted with fraction 1. This study suggests that these antigens might be adaptable to the detection of human gonococcal antibody, especially in the female.


Effect of pH and chelating agents on the heat resistance and viability of Salmonella typhimurium Tm-1 and Salmonella senftenberg 775W in egg white. Supplementation of egg white at pH 8.9 with 5 mg of disodium ethylenediaminetetraacetic acid (EDTA) per ml resulted in a kill of Salmonella typhimurium Tm-1 of greater than 10(6) per ml after 28 days at 2 C. While at 28 C, supplementation with 7 mg of EDTA per ml resulted in approximately a 10(6) kill in less than 24 hr. Kena supplementation at 40 mg/ml of egg white resulted in a kill of S. typhimurium Tm-1 of greater than 10(6) after approximately 60 hr of storage at 28 C. This is in contrast to no reduction in viable count in unsupplemented egg white stored at 2 C and a 100-fold increase in viable count in that stored at 28 C. Supplementation of egg white with EDTA at 7 mg/ml or with Kena at 10 mg/ml also affected the heat resistant characteristics of the two organisms at 52.5 C, reducing the time required to kill 90% of the population (D value) at any pH by a factor of 2 to 6. There was a synergistic effect between EDTA and lactic acid when lactic acid was used to adjust EDTA-supplemented egg white to an acidic pH (5.3) which greatly decreased the heat resistance of Salmonella senftenberg 775W (from 100D to D).


In vitro activity of coumermycin A1 against Mycobacterium tuberculosis var. hominis. Coumermycin A(1) exhibited in vitro activity against eight strains of Mycobacterium tuberculosis var. hominis. All eight strains were inhibited by levels of 2.5 mug/ml, and six of the eight strains were inhibited by levels of 0.3 mug/ml or less.


Automatic assessment of respiration during growth in stirred fermentors. An apparatus for the continuous and automatic measurement of respiration during growth of micro-organisms in stirred aerated culture is described. The effluent atmosphere from the culture vessels is passed through commercially available oxygen and carbon dioxide gas analyzers, and their electrical output is fed to a multipoint recorder. The apparatus has been used to measure the respiration of Escherichia coli, Pseudomonas aeruginosa, Bacillus thuringiensis, and Methylococcus capsulatus during growth on complex and defined media. In addition, pH values and dissolved oxygen concentrations were measured. Growth of P. thuringiensis in glucose-limited media showed unexpected interruptions in the oxygen consumption curves which resembled diauxic growth; growth of B. thuringiensis in complex media showed similar discontinuities of respiration. These results are explained as the sequential utilization of preferred substrates which, in the case of P. aeruginosa were provided as transient intermediates of glucose.


Complement-fixing antigen from BHK-21 cell cultures infected with lymphocytic choriomeningitis virus. Infection of BHK-21 cells with lymphocytic choriomeningitis (LCM) virus resulted in the production of significant titers of complement-fixing (CF) antigen. The antigen was spontaneously released from the cells, but the highest titer of 1:16 was recovered by disruption of the infected cells by freeze-thawing in tryptose phosphate broth. The antigen could be partially separated from infectious virus by centrifugation. Furthermore, it was possible to detect LCM virus infection of cell cultures by the production of the CF antigen, but this method proved less sensitive than titration by intracerebral inoculation of mice. The CF antigen from cell cultures was at least as sensitive and specific as the reference antigen prepared from infected guinea pig spleen.


The isolation and characterization of deoxyribonucleoproteins from human spleen.


Use of mitomycin C to distinguish Brucella abortus field strains from the vaccinal Brucella abortus strain 19. Field strains of Brucella abortus were resistant to mitomycin C, whereas strain 19 was sensitive; therefore, the antibiotic was used to distinguish B. abortus strain 19 from other strains.


The fine structure of Neobursaridium gigas (Balech).


Serological characteristics of Streptomyces species using cell wall immunizing antigens.


The effects of serum components on the agglutination of Gram-negative bacteria.


The use of fluorescein isothiocyanate in the determination of the bacterial biomass of grassland soil.


Serologic diagnosis of active toxoplasmosis complicating malignant diseases. Usefulness of IgM antibodies and gel-diffusion.


Effect of oculomotor nerve stimulation on outflow facility and pupil diameter in a monkey (Cercopithecus ethiops).


Antigenic similarities of alpha s1-caseins A, B, and C.


Craniomandibular bone changes in rhesus monkeys induced by condylectomy.


The Austin, Texas, diphtheria outbreak. Clinical and epidemiological aspects.


Third Annual Australian Tuberculosis Conference.


Uterine blood flow.

Uterine blood flow.


Ethanol-induced release of acetaldehyde from blood and its effect on the determination of acetaldehyde.


Hypergammaglobulinemia, secondary macroglobulinemia and paraproteinemia in leprosy.


Pesticides and the reproduction of birds.


Periodontal effects of root perforations before and during endodontic procedures.


Mandibular-fracture repair in monkeys.

Mandibular-fracture repair in monkeys.


Somatic changes in the fetus. ORO-672.

Somatic changes in the fetus. ORO-672.


Accumulation and rlease of thyroid hormones by the normal and diseased human liver.


Comments on approaches to moderating the increases in medical care costs.


Possible new concepts relating prostaglandins to various ocular functions.


Throat streptococci in rheumatoid arthritis.


The effect of histaminase inhibition on the concentration and distribution of 14C histamine in blood during pregnancy.


Effect of drugs used in psychoses on cerebral dopamine metabolism. 1. Chlorpromazine 15 mg/kg, given daily to cats for 2 weeks, produced a rise in homovanillic acid (HVA) content of the caudate nucleus, whereas the same dose of thioridazine lacked this effect. Of these two drugs, only chlorpromazine causes a high incidence of drug-induced Parkinsonism in man.2. In the mouse, chlorpromazine, thioridazine and haloperidol increased striatal concentrations of HVA and accelerated the disappearance of dopamine (DA) after inhibition of catecholamine synthesis with alpha-methyltyrosine. Low doses of the three compounds increased, whereas high doses reduced, the concentration of DA in the striatum. In their effects on the DA metabolism of the mouse, chlorpromazine and thioridazine had the same potency, but haloperidol was between 10 and 100 times more active than the other two drugs. In producing hypothermia and sedation, the three compounds were equiactive.3. Oxypertine, another drug apt to produce Parkinsonism in man, caused a severe reduction in striatal DA and hypothalamic noradrenaline (NA). Though the clinical signs produced in the mouse were indistinguishable from those seen after the same dose of chlorpromazine, the biochemical changes in the brain were thus quite different.4. Though all the drugs used caused temporary motor disabilities in animals, these bore no resemblance to human Parkinsonism, even when treatment was continued for 7 weeks or more as it was in cats and monkeys. The latter were treated with chlorpromazine 7.5 mg/kg daily, a dose chosen to avoid loss of weight and which may have been too small to produce toxic side-effects. It caused no changes in striatal DA turnover.5. Even at the high dose of 50 mg/kg, phenoxybenzamine did not increase DA turnover in mouse brain, but it sedated the mice as did the tranquillizers.6. Atropine sulphate, 25 mg/kg, reduced the HVA content of mouse striatum and partially antagonized the rise in HVA produced by phenothiazines. The effect was surmountable. Possible modes of action of atropine are discussed.7. At present we know of two types of biochemical changes which may occur in the brain of animals after treatment with drugs apt to cause Parkinsonism in man: a loss of cerebral catecholamines, as seen after reserpine or oxypertine, or an increase in turnover of DA as after phenothiazines and butyrophenones.


Methods for detecting anti-anxiety drugs using baboons (Papio cynocephalus).


Viruses of amphibia.

Viruses of amphibia.


Suppression of portal venous insulin concentration by epinephrine i the conscious monkey.


A study of the rhythm of petit mal absences in children in relation to prevailing situations. The use of EEG telemetry during psychological examinations, school exercises and periods of inactivity.


Co-existence of focal and bilateral diffuse paroxysmal discharges in epileptics. Clinical-electrographic study.


The long-term oral use of valium (diazepam) in epilepsy.


Notes on tertiary platyrrhine monkeys and description of a new genus from the late Miocene of Colombia.


Maintenance breeding, and development of marmoset monkeys (Callithricidae) in captivity.


Excretion of gonadotrophin by the pregnant baboon (Papio cynocephalus).


The chromosomes of Humboldt's woolly monkey (Lagothrix lagothricha, Humboldt 1812).


Haematological observations on the squirrel monkey.


The menstrual cycle and sexual behaviour in a troop of free ranging chacma baboons (Papio ursinus).


Dominance structure of the Nilgiri langur (Presbytis johnii) of South India.


Activity patterns in pigtail monkey groups.


Vaginal cytology and reproduction in the squirrel monkey (Saimiri sciureus).


Colobomatous malformations of the ocular globe.


Experimental hydrocephalus. 1. A technique for producing obstructive hydrocephalus in the monkey.


Experimental hydrocephalus. 2. Gross pathological findings in acute and subacute obstructive hydrocephalus in the dog and monkey.


Experimental hydrocephalus. 3. Light microscopic findings in acute and subacute obstructive hydrocephalus in the monkey.


Enzymic methods of analysis.

Enzymic methods of analysis.


A note on models and explanation in biology.


Delivery of personal health services to the public.


The picture of health: the private sector in health education and health care.


Changing patterns of costs and structure in the health sector.


Current trends in British medical planning.


The pulmonary ultrastructure in septic shock.


A new apparatus for the dissolution of renal calculi. Experimental research.


Treatment of angina pectoris with dipyridamole: a double-blind study.


Contraction of protoplasm. 3. Cinematographic analysis of the contraction of some heterotrichs.


The characterization and occurrence of an Sf 20 serum lipoprotein.


The role of traditional public health services in the age of medicare.


Contraction of protoplasm. IV. Cinematographic analysis of the contraction of some peritrichs.


Heritable mass conversion of a mutant penicillinase-negative culture of Bacillus cereus to a positive fully de-repressed state.


Incorporation of thymine into prototrophic and thymine-dependent mutants of Bacillus anthracis.


Dietary lactobacilli and amine production in the alimentary tract of the pig in relation to weaning and postweaning diarrhoea.


The somatic antigens of two strains of Rhizobium trifolii.


Virulence and host specificity of TRIC agents.


Is there a malaria toxin?

Is there a malaria toxin?


Clinical uses of metal-binding drugs.

Clinical uses of metal-binding drugs.


The 5'-ends of the DNA of defective bacteriophages of Bacillus subtilis.


Freeze-etching and direct observation of freezing damage.


Initiation of DNA replication in Bacillus subtilis. II. Linkage between termini of parental strands and origins of daughter strands.


Metabolic fate of epoxycholesterol in the rat.


A marine botanist looks at the problem.

A marine botanist looks at the problem.


Antimicrobial agents from the sea.

Antimicrobial agents from the sea.


Demonstration in sarcoma patients of anti-tumor antibodies which fix only human complement.


Gliding in a blue-green alga: a possible mechanism.


Localization of acetylcholinesterase by immunofluorescence in eel electroplax.


Entropy of microbial growth.

Entropy of microbial growth.


Effects of inert gases and nitrous oxide on the radiation sensitivity of HeLa cells.


Post irradiation hibernation effects on the radiation response of the ground squirrel.


Survey of unmet medical needs of children in six counties in Florida.


Factors influencing enrollment in Head Start classes in Hawaii.


Urinary deoxycytidine levels: an index of radiochemical protection in sublethally irradiated rats.


A genetic within-strain difference in radiation sensitivity in the RFM mouse.


The decline of individualism.

The decline of individualism.


Continuance of contraception post partum by patients of Cook County Hospital.


Radiation response of L cells treated with dimethyl sulfoxide.


Measures of longevity of American Indians.


Trends of sporadic leptospirosis in Florida.


Tyrosinase induction by gamma-radiation in neurospora.


Response of Photobacterium fischeri H-2 to the pulsed electron beam.


Social design and operations research.

Social design and operations research.


Hyperlipidemia as a prognosticator of abnormal glucose tolerance.


Must mammalian cells degrade DNA in order to repair radiation injury?


Growth pattern changes in a mouse leukaemia following total body irradiation (TBI).


Reaching for accountability in community practice.


Determination of measles immunity after a mass immunization campaign.


Biological effects of small doses of radiation.


Delta rays and RBE theory.

Delta rays and RBE theory.


Urban and regional foundations for health planning.


A spectrophotometric Dewar flask with integral light shield.


Use of the Blunck-Leisegang energy-loss theory in correlating radiobiological effects.


Quantitative aspects of recovery.

Quantitative aspects of recovery.


An analogue system for rapid treatment planning.


Measurement of changes in the oxygen and carbon concentration of tissues during fractionated high energy x-ray treatment.


Breeding and pregnancy in rhesus monkeys used for teratological testing.


L-form of Pseudomonas aeruginosa the etiologic agent of bacterial endocarditis in a dog.


Phencyclidine hydrochloride as a general anesthetic.


Degradation and reassembly of a human serum high-density lipoprotein. Evidence for differences in lipid affinity among three classes of polypeptide chains.


Differences among the ribosomal RNA's of various mammals.


Defined conditions for DNA extraction from Bacillus subtilis spores.


Connexions of the somatic sensory cortex of the rhesus monkey. 3. Thalamic connexions.


Determination of thyroxine-binding globulin. A simplified procedure utilizing dextran-coated charcoal.


Distant effects of a focal epileptogenic process.


An automated method for serum calcium utilizing ethylene diamine tetraacetic acid.


Somesthetic discrimination learning after partial nonsensorimotor lesions in monkeys.


Dorsolateral frontal ablations and response processes in monkeys.


Squirrel monkey heart rate during formation of status orders.


Evidence for behavioral impairment following prefrontal lobectomy in the infant monkey.


Effect of bilateral frontal lobectomy on social preferences of rhesus monkeys.


Relationship of ITI to acquistion of serial visual discriminations following temporal rhinencephalic resection in monkeys.


Fast neutron central axis depth dose equation.


The effect of haematocrit and anticoagulants on the rate of packing of erythrocytes by a centrifuge.


Tubular structures associated with the endothelial endoplasmic reticulum in glomerular capillaries of Rhesus monkey and nephritic man.


Immunological pregnancy diagnosis in the mare.


The effect of ethanol and nitroglycerin on platelet aggregation.


Thermal stimulation of Bacillus megaterium glucose-6-phosphate dehydrogenase.


Differentiation between heat resistance and octyl alcohol resistance of the cells of Bacillus cereus T.


Edeine. IV. Structures of the antibiotic peptides edeines A1 and B1.


Structure of porcine thyrotropin releasing hormone.


Limitations of the enzymic-isotope dilution method for the assay of metabolites.


Ammonia incorporation in Hydrogenomonas eutropha.


A few comments on the rising costs of medical care.


Some facets of the economics of health care delivery.


Brain temperature and thermosensitivity of nerve cells in monkey.


Single unit studies on acute and chronic epileptic foci, their surrounds and their mirror foci in cats and monkeys.


Assessment of the cerebral microvasculature in seizure disorders.


A test for separating secondary from primary bilateral synchrony in epileptic subjects.


Immunopathological mechanisms of allergic neuritis in animals, primates, and man.


A summary of current concepts of the dynamics of head injury.


Oral prehension and impaired visual discrimination after basal temporal lobectomy.


Mechanisms involved in grand mal seizures.


Studies of peptide maps of ceruloplasmin in Wilson's disease.


Antibody to human myosin in man.

Antibody to human myosin in man.


Aeromedical consultation service case report: clinical entities mimicking vasovagal syncope.


Penetration of gentamicin into the aqueous humor of human eyes.


The relationship of transplacental haemorrhage to rhesus immunisation.


Imbalancing of hemic calcium, magnesium, potassium, and sodium by rations and by perenteral injections of ethylenediaminetetraacetate dipotassium salt, potassium chloride, and aldosterone inhibitor. Comparison of cattle held at 2,745 and at 1,372 meters elevation.


Oral ampicillin in gonorrhoea. Clinical evaluation.


Immunofluorescent method for diagnosis of gonorrhoea in women.


Urinary excretion of hydroxyproline-containing peptides in man, rat, hamster, dog and monkey after feeding gelatin.


Trimethoprim-sulphamethoxazole in the treatment of non-gonococcal urethritis and gonorrhoea.


The natural history of malocclusion.

The natural history of malocclusion.


Thyroid cytoplasmic ribonucleoprotein particles of very high size containing messenger-like and ribosomal ribonucleic acids.


The quantitative measurement of DNA hybridization from renaturation rates.


The determination of molecular weight of bacterial genome DNA from renaturation rates.


Urate oxidase in primate phylogenesis.

Urate oxidase in primate phylogenesis.


Gastroscopic color photography in cancer chemotherapy.


The gastric mucosa of patients in diabetic ketoacidosis: a gastrocamera study.


Histochemical distribution of acetylcholinesterase and simple esterases in the brain of squirrel monkey (Saimiri sciureus).


Amine mechanisms in enterochromaffin and enterochromaffin-like cells of gastric mucosa in various mammals.


Sulfa-drug resistance and serologic studies of a meningococcal meningitis epidemic.


Preservation of glycogen in decalcified hard tissues.


Antigenic analysis and vaccines.

Antigenic analysis and vaccines.


Potassium therapy and gastrointestinal lesions.


An adult case of bladder diverticula without urinary obstruction.


Reactions of sipunculid coelomic fluid with erythrocytes.


Active bone marrow distribution in the monkey.


Pollution: enriching the waters.

Pollution: enriching the waters.


Biological nitrogen fixation.

Biological nitrogen fixation.


Australia antigen-antibody complexes.

Australia antigen-antibody complexes.


A study in New Zealand mortality. 6. Epidemic diseases.


The effects of acids, alkalies, and chelating agents on dentine permeability.


The function of prostaglandins in reproduction.


Cation and sterol effects on growth of Phytophthora parasitica var. nicotianae.


Possible association of a polysaccharide and an antibiotic with the disease cycle of Cephalosporium stripe.


Serological variation and identification of Pseudomonas lachrymans and other phytopathogenic Pseudomonas nomenspecies.


A triple tetracycline combination.

A triple tetracycline combination.


Disinfection of Goldmann's applanation tonometer prism by means of ultraviolet light.


Pseudomonas infections in a paediatric surgical unit.


Pulmonary diffusing capacity in the dog as influenced by anesthesia and ventilatory regime.


Inhibitory effect of lysolecithin on bacterial growth.


An activator of C'ls to C'l esterase in the macroglobulin fraction of human sera.


Presence and survival of Marek's disease agent in dust.


Passive hemagglutination test with fowlpox virus.


The detection by immunofluorescence of casein in rat mammary glands.


Passive immunisation against Gram-negative bacilli in burns.


Chromosome studies of virus-infected semi-continuous human embryonic liver cells.


A micromethod for determination of antibiotics in serum.


Immunological tolerance and the significance of cell-mediated immunity in virus infections.


Effect of dorsal rhizotomy on postural tremor in the monkey.


Single unit activity in the frontal eye fields of unanesthetized monkeys during eye and head movement.


CCHPA pushes for change in health system and costs.


Chromosomal aberrations in monoclonal gammopathies without clinical features of plasma cell myeloma or macroglobulinaemia.


A comparative study of cranial growth in Homo and Macaca.


The small pyramidal neuron of the rat cerebral cortex. The perikaryon, dendrites and spines.


The role of ovarian hormones in the sexual preference of rhesus monkeys.


Studies of Australia-SH antigen in sporadic viral hepatitis in London. Sera from 87 patients with acute sporadic viral hepatitis were tested for the presence of the Australia-SH antigen. Forty-three were positive by the complement-fixation test, but only 24 of these reacted in the gel-diffusion test. The antigen was equally distributed in infectious and serum hepatitis. The relationship of naturally occurring antigen-positive hepatitis to the Willow-brook MS-2 type is discussed.


Task Force job just beginning. Improved procedures, taxpayer rights stressed.


Intrabronchial metallic mercury.

Intrabronchial metallic mercury.


Cellular pathophysiology of focal epilepsy.


A developmental study of cognitive and personality characteristics associated with haemolytic disease of the newborn.


Consumer views.

Consumer views.


The relationship of ribosomal activity to lymphoid tissue histology in Borrelia infection.


Financing health care.

Financing health care.


Pollen-white cell interactions.

Pollen-white cell interactions.


Pathogenic bacteria and their sensitivity patterns in a hospital population of geriatric patients with chronic disease.


Group dynamics and the domiciliary health team.


Determination of specific cerebral impedance and cerebral current density during the application of diffuse electrical currents.


Evaluation of a number of ancillary tuberculin tests in cattle.


The pharmacological basis of antianginal drugs.


Complications of surgery on duodenal diverticula.


Endotoxin shock.

Endotoxin shock.


Coagulation and fibrinolysis in prematurely delivered mothers and their premature infants.


The urinary excretion of oestriol in pregnancies with Rh-immunisation.


Argon laser slit-lamp retinal photocoagulation.


Enzymic synthesis of spermine in rat prostate.


Tri-malleolar fracture/dislocation of the ankle.


Activation of tyrosinase in microsomes and melanosomes from B16 and Harding-Passey melanomas.


Isolation of an acute phase alpha-2-glycoprotein from rat inflammatory serum.


A radioimmunoassay for rhesus monkey luteinizing hormone (RhLH).


Evidence of independent secretion of prolactin and growth hormone in vitro by adenohypophyses of rhesus monkeys.


Porcine thyrotropin releasing hormone is (pyro)glu-his-pro(NH2).


Direct dark-ground printing of precipitation bands.


Soluble nitrogenase from vegetative cells of the blue-green alga Anabaena cylindrica.


Delayed hypersensitivity in Trypanosoma cruzi infection.


Double blind trial of long-term anticoagulant treatment after myocardial infarction.


Haemodynamic effects of propranolol and nitroglycerin in normal subjects during submaximal exercise.


Zinc 65 distribution and movement in frog skeletal muscle and tendon.


Formation and localization of the alkaline phosphatase of Escherichia coli.


Purification and molecular properties of placental and intestinal alkaline phosphatases.


Placental alkaline phosphatase and pregnancy.


Serologic comparisons of the carbonic anhydrases of primate erythrocytes.


Discussion paper studies on serum alkaline phosphatase isoenzymes.


Thyroxine-protein interactions. VI. Structural requirements for binding of benzene derivatives to thyroxine-binding sites on human serum albumin.


The messenger ribonucleic acid content of Bacillus subtilis 168. Bacillus subtilis 168 messenger RNA was determined by DNA-RNA hybridization techniques, with denatured DNA immobilized upon cellulose nitrate membrane filters. The following results were obtained. (1) Cultures of B. subtilis, growing exponentially in enriched glucose-salts medium at 37 degrees , incorporated [5-(3)H]uracil into both ribosomal and messenger RNA fractions without the kinetic delay expected from the presence of the intracellular nucleotide pools. (2) However short the time of labelling with exogenous labelled uracil (down to 7sec.), 32-36% of the rapidly labelled RNA was messenger RNA and 68-64% was an RNA with the hybridization characteristics of ribosomal RNA. Analysis of the apparent nucleotide base composition of total (32)P-labelled rapidly labelled RNA and the two RNA fractions separated by hybridization at a DNA/RNA ratio 5:1 confirmed this finding. Of the rapidly labelled RNA, 31% readily hybridized with DNA at low DNA/RNA ratios and had an apparent base composition like that of the DNA, whereas 69% was hybridized only at low efficiency at low DNA/RNA ratios and had a composition identical with that of ribosomal RNA. (3) In cultures dividing every 48min. at 37 degrees , kinetic analysis of RNA labelled over a 20min. period showed that the average life-time of messenger RNA was 2.7-3.0min. and that its amount was 3.0% of the total RNA. (4) The hybridization of (3)H-labelled randomly labelled RNA with DNA at a DNA/RNA ratio 5:1 showed that 2.9% of the randomly labelled RNA had the characteristics of messenger RNA. (5) Experiments carried out as described by Pigott & Midgley (1968) indicated that hybridization at low DNA/RNA ratios (5:1) effectively accounted for all the messenger RNA in a given specimen. The efficiency coefficient of RNA hybridization lay within the range of 90-95% input, if an excess of DNA sites was offered for RNA binding. (6) These measurements are compared with other results obtained by different methods, and reasons for any major disagreement are suggested.


Oligomycin-induced energization of submitochondrial particles.


Presence and spatial localization of carbonic anhydrase in rat liver mitochondria.


Manganese in chloroplasts.

Manganese in chloroplasts.


Investigation of the cross-reaction of rat prolactin with antisera to ovine prolactin.


Tracer studies of the fate of steroid hormones in the baboon.


Effect of electrical stimulation of the hypothalamus on thyroid secretion in monkeys.


Extremity injuries produced in motor vehicle collisions.


Studies on the release of bradykinin by the splanchnic circulation during endotoxic shock.


Antimicrobial polypeptide synthesized by Mucor pusillus NRRL 2543.


Studies on the electrophoretic mobility of troponin in the presence of Ca2+, Mg2+, (Mg-ATP)2-.


The effect of sulfhydryl compounds and mercurials on the swelling rate of cells of Pseudomonas aeruginosa in sodium and potassium buffers.


Free cholesterol exchange in vitro: a comparison of endogenous and exogenous cholesterol.


Toxicity of combinations of glyceryl trinitrate and sympathomimetics.


Stimulation of DOPA14C incorporation into melanoma tissue by chlorpromazine and reserpine.


Horizontal nystagmus of rhesus monkeys.

Horizontal nystagmus of rhesus monkeys.


Macroamylasemia: serum and urine amylase characteristics.


Effects of secretor, blood, and serum groups on isoantibody and immunoglobulin levels.


Structural, ultrastructural, and permeability changes in the ependyma and surrounding brain favoring equilibration in progressive hydrocephalus.


Genetic linkage relations of the loci for 6-phosphogluconate dehydrogenase and adenosine deaminase in man.


The metabolic fate of amphetamine in man and other species. 1. The fate of [(14)C]amphetamine in man, rhesus monkey, greyhound, rat, rabbit, mouse and guinea pig has been studied. 2. In three men receiving orally 5mg each (about 0.07mg/kg), about 90% of the (14)C was excreted in the urine in 3-4 days. About 60-65% of the (14)C was excreted in 1 day, 30% as unchanged drug, 21% as total benzoic acid and 3% as 4-hydroxyamphetamine. 3. In two rhesus monkeys (dose 0.66mg/kg), the metabolites excreted in 24h were similar to those in man except that there was little 4-hydroxyamphetamine. 4. In greyhounds receiving 5mg/kg intraperitoneally the metabolites were similar in amount to those in man. 5. Rabbits receiving 10mg/kg orally differed from all other species. They excreted little unchanged amphetamine (4% of dose) and 4-hydroxyamphetamine (6%). They excreted in 24h mainly benzoic acid (total 25%), an acid-labile precursor of 1-phenylpropan-2-one (benzyl methyl ketone) (22%) and conjugated 1-phenylpropan-2-ol (benzylmethylcarbinol) (7%). 6. Rats receiving 10mg/kg orally also differed from other species. The main metabolite (60% of dose) was conjugated 4-hydroxyamphetamine. Minor metabolites were amphetamine (13%), N-acetylamphetamine (2%), norephedrine (0.3%) and 4-hydroxynorephedrine (0.3%). 7. The guinea pig receiving 5mg/kg excreted only benzoic acid and its conjugates (62%) and amphetamine (22%). 8. The mouse receiving 10mg/kg excreted amphetamine (33%), 4-hydroxyamphetamine (14%) and benzoic acid and its conjugates (31%). 9. Experiments on the precursor of 1-phenylpropan-2-one occurring in rabbit urine suggest that it might be the enol sulphate of the ketone. A very small amount of the ketone (1-3%) was also found in human and greyhound urine after acid hydrolysis.


Species differences in the aromatization of quinic acid in vivo and the role of gut bacteria. 1. The fate of (-)-quinic acid has been investigated in 22 species of animals including man. 2. In man and three species of Old World monkeys, i.e. rhesus monkey, baboon and green monkey, oral quinic acid was extensively aromatized (20-60%) and excreted in the urine as hippuric acid, which was determined fluorimetrically. 3. In three species of New World monkeys, i.e. squirrel monkey, spider monkey and capuchin, in three species of lemurs, i.e. bushbaby, slow loris and tree shrew, in the dog, cat, ferret, rabbit, rat, mouse, guinea pig, hamster, lemming, fruit bat, hedgehog and pigeon, oral quinic acid was not extensively aromatized (0-5%). 4. In the rhesus monkey, injected quinic acid was not aromatized, but largely excreted unchanged. 5. In rhesus monkeys pretreated with neomycin to suppress gut flora, the aromatization of oral quinic acid was considerably suppressed. 6. In rats and rhesus monkeys [(14)C]quinic acid was used and this confirmed its low aromatization in rats and its high aromatization in the monkeys. 7. Shikimic acid given orally was excreted as hippuric acid (26-56%) in rhesus monkeys, but not in rats. 8. The results support the view that quinic acid and shikimic acid are aromatized by the gut flora in man and the Old World monkeys.


Cross-reactivity studies of horse, goat and rabbit anti-lymphocyte globulin.


Heterophile antibodies in Nigerian sera.


Suppressant effect of procarbazine and its comparison with anti-lymphocyte serum in adjuvant-induced polyarthritis in rats.


Failure to transfer haemolytic anaemia or glomerulonephritis with cell-free material from NZB mice.


A study of the potential nephrotoxicity of heterologous anti-lymphocyte serum.


Electroencephalographic characteristics of brain cooling and rewarming in monkey.


Behavioral indices for neural systems involved in pain and fear responses of monkeys.


Noxious oxygen.

Noxious oxygen.


Intrauterine administration of progesterone by a slow releasing device.


C5 chemotactic fragments produced by an enzyme in lysosomal granules of neutrophils.


Allotype suppression in alpha-2alpha-2 homozygous rabbits fostered in uteri of alpha-2-immunized alpha-1alpha-1 homozygous mothers and injected at birth with anti-alpha-2 antiserum.


Studies on brain antigens. 3. Purification and characterization of a water-soluble bovine antigen specific for nervous tissue (alpha-BASNT).


The dissociation of human serum 7S and bovine colostrum immune globulins into subunits by reduction-alkylation-succinylation.


Metal cation requirements for phytohemagglutinin-induced transformation of human peripheral blood lymphocytes.


Antibody response in rhesus monkeys and guinea pigs to inoculation with RNA derived from antigenically stimulated cell-free systems.


Serological comparison of ribosomal and viral components.


Hand preference and cerebral predominance in 24 rhesus monkeys.


Neuroschisis and human embryonic maldevelopment. New evidence on anencephaly, spina bifida and diverse mammalian defects.


Relationship of golgi thiaminepyrophosphatase and lysosomal acid phosphatase to neuromelanin and lipofuscin in cerebral neurons of the aging rhesus monkey.


Polyphloretin phosphate - a selective antagonist for prostaglandins F1 alpha and F2 alpha.


Ileal bypass in treatment of hyperlipidemia: rationale and present status.


A uniformed services Academy of Medicine: commentary on Mr. Hebert's proposal.


Progress report: the blood program in Vietnam.


Cancer detection clinic day at an Air Force Base hospital.


Polymyalgia rheumatica in patients of military age.


The utilization of a physicians' nurse assistant in the gynecological cancer detection clinic.


Scrub typhus in Vietnam: experience at the 8th Field Hospital.


Maternal-child care among the Chippewa: a study of the past and the present.


Priapism in leukemia: a report of two cases.


Case for diagnosis.

Case for diagnosis.


Carcinoma of the female urethra.

Carcinoma of the female urethra.


Observations in the use of a syringe-needle attachment unit for the preparation of prepackaged parenterals.


The diagnosis of hypertension.

The diagnosis of hypertension.


A computerized approach to evaluation of unusual preventive medicine data.


An inquiry into the nature of wounds resulting in killed in action in Vietnam.


Identity of very low density lipoprotein apoproteins of plasma and liver Golgi apparatus. In the rat, very low density lipoproteins isolated from hepatocyte Golgi apparatus, liver perfusates, and whole plasma appear identical in many respects. With specific immunochemical techniques and polyacrylamide-gel electrophoresis it can be demonstrated that the very low density lipoproteins from all three sources contain the same major lipoprotein apoproteins.


Epilepsy in Nigerians. Study of 522 patients.


Deposits of granular material in mitochondria. Electron microscopic observations in neoplastic cells oa a malignant melanoma of the human uveal tract.


Effect of Trenimon on the ultrastructure of Sertoli cells in the mouse.


Myocardial ultrastructural modification in rat heart-lung preparation failed under two different haemodynamic conditions.


Quantitative cytochemical investigations on NK/Ly mouse ascites tumour cells treated with Degranol. I. Feulgen cytophotometric and polarization optical investigations.


Quantitative cytochemical investigations on NK/Ly mouse ascites tumour cells treated with Degranol. II. Fast green cytophotometric and interference microscopic investigations.


Trichromatic mechanisms in single cortical neurons. By chromatic adaptation, all three cone mechanisms of rhesus monkey vision can be identified in single neurons of striate cortex. This trichromatic inter-action occurs in cells sensitive to color and indicates that striate cortical cells tend to be more wavelength discriminating than cells at lower stages of the primate visual system.


Determination of growth rates by tetracycline-labelling in guinea pig maxillas.


Interactions of hormonal steroids with nucleic acids. 3. Role of polymer structure.


Stimulation of soluble ribonucleic acid methylase activity by polyamines.


Carbamyl phosphate biosynthesis in Bacillus subtilis.


Indirect evidence for the production of superoxide anion radicals by pig kidney diamine oxidase.


Properties and sub-cellular distribution of two sulfatases which degrade adenosine 5'-phosphosulfate.


The effects of x-irradiation on mammalian oocytes in organ culture.


Interlocked DNA rings. II. Physicochemical studies.


Some properties of the 695 millimicron band of Pseudomonas cytochrome C.


Hemocytes, hemolymph, and defense reactions in crustaceans.


Influence of endotoxin (51cr) structure on blood clearance in the absence of specific antibodies.


Localization by peroxidase-labeled antibodies of bovine chymotrypsinogen.


Experimental immune myositis in guinea pigs.


Proposals for achieving more adequate health care for children and youth.


Conference on health services for children and youth. Synthesis and recommendations.


Components of existing and proposed U.S. health insurance programs. October, 1969.


Present strengths and weaknesses in current systems of comprehensive health services for children and youth.


Isoniazid-resistant strains of M. tuberculosis.


The disinfection of swimming pool water. II. A field study of the disinfection of public swimming pools.


The therapeutic effect of homologous and heterologous antitoxins in experimental diphtheria and tetanus.


Fine structure of the otolithic membrane in the squirrel monkey.


Diabetic ketoacidosis. A review of cases at a university medical center. Twenty-five cases of diabetic ketoacidosis were studied retrospectively with respect to clinical characteristics and results of therapy. In this series (as with all 88 patients admitted in the last five years with a diagnosis of diabetic ketoacidosis) there were no deaths. Infection was found to be the most common precipitating event, documented by physical findings and cultures in one-third of these cases. In about two-thirds of the cases, electrocardiograms which were read as abnormal on admission reverted to normal after therapy. In all patients serum potassium levels decreased from admission values; one patient became symptomatically hypokalemic. Low serum potassium levels on admission and early vigorous bicarbonate therapy are emphasized as major predisposing factors of symptomatic hypokalemia. None of the patients had overt hyperosmolar coma, lactic acidosis or cerebral edema during therapy.


Activity of Bacillus cerus proteinases in milk.


Problems of Neisseria meningitidis infection.


The effect of phytohemagglutinin on phagocytosis in the mouse peritoneal cavity.


Geriatric and psychogeriatric patient requirements in Perth, Western Australia.


Discrepancies in the intracellular behavior of three strains of tobacco mosaic virus, two of which are serologically indistinguishable.


The relation of tobacco mosaic virus x-protein to amorphous cellular inclusions (X-bodies).


Postoperative management of the severe oral cripple.


Thromboembolism, oral contraceptives, and cigarettes.


p,p'-DDT: effect on calcium metabolism and concentration of estradiol in the blood. Ringdoves given 10 parts per million p,p'-DDT showed a decrease of estradiol in the blood early in the breeding cycle and egg-laying was delayed. There was also a decrease in deposition of medullary calcium and in eggshell weight. Injection of p,p'-DDE (150 milligrams per kilogram of body weight) caused reduction of eggshell weight and inhibition of carbonic anhydrase in the oviduct.


DDT-induced inhibition of avian shell gland carbonic anhydrase: a mechanism for thin eggshells. The shell-forming glands of Japanese quail fed p,p'-DDT or p,p'-DDE had carbonic anhydrase activity 16 to 19 percent lower than shell glands of quail on a diet free of pesticides.


The use of a triple lumen gastric tube for suction and feeding.


Pharmacological studies on 2-methyl-3(2'-methyl-4'-chlorophenyl)-5-chloro-4[H)-quinazolinone (SL-164).


4-phenylglioxylidenamino-antipyrine with antiphlogistic and analgesic activity.


The whey proteins of pig's milk. Isolation and characterization of a beta-lactoglobulin.


The separation of clotting factors II (prothrombin) and IX (plasma thromboplastin component) by isoelectric focusing.


The proteins in membrane and cytoplasmic ribosomes of Streptococcus fecalis. An extra protein in the 50-S subparticles of cytoplasmic ribosomes.


Further separation of the apoproteins of the human plasma very low density lipoproteins.


Replica demonstrations of the tenosynovial lubricous membrane lining the serous cavity of the tendon system.


Immunological investigations on the evolution of beta 2-glycoproteins 2 and 3, IgD and inter-alpha-trypsininhibitor.


Uptake of calcium by subcellular fractions isolated from ouabain-treated cerebral tissues.


The effect of temperature on the viability of R. prowazeki in the N-7 buffer.


Properties of the Hong Kong influenza virus. I. General characteristics of the Hong Kong virus. Biological properties of the Hong Kong influenza variants are described in relation to the isolation and identification of strains, sensitivity to nonspecific inhibitors, and serological diagnosis. Hong Kong virus strains were readily isolated in both eggs and monkey-kidney tissue cultures and were identified by haemagglutination-inhibition (HI) with chicken erythrocytes. The morphology, soluble antigen, and neuraminidase of Hong Kong isolates were similar to those of earlier A2 Asian influenza strains. Hong Kong haemagglutinins were related in varying degrees to previous human A2 and animal influenza viruses.The sensitivity of Hong Kong isolates to nonspecific haemagglutination inhibitors in serum varied widely. From least to most inhibitory, the ranking of sera of 8 animal species tested was: monkey, goat, chicken, human, rabbit, ferret, guinea-pig and horse. The same sera were treated with heat, trypsin, periodate, receptor-destroying enzyme and kaolin to determine the most effective way of removing nonspecific inhibitors. The results varied with the animal species involved.The A2 Asian antigen was nearly as effective as Hong Kong antigen in detecting antibody rises by HI tests. HI tests with either antigen were more efficient than complement-fixation tests for serodiagnosis of Hong Kong influenza, but for maximum efficiency both tests were required.


The roles of early alert and of adjuvant in the control of Hong Kong influenza by vaccines. The major antigenic changes in influenza A virus that occur at 10-year intervals reduce the effectiveness of existing vaccines and pose a problem for the control of pandemics by vaccination. These difficulties may be obviated in two ways. First, by detecting the emergence of a new variant sufficiently ahead of its general spread for it to be possible to produce a corresponding vaccine in good time; the World Health Organization is widely depended upon for this early warning. Secondly, by the improvement of existing vaccines to increase and broaden their antigenicity; here, their modification by, for instance, adjuvant 65 may contribute significantly.


Chimerism for three genetic systems in tetraparental mice.


Long-tm treatment of Ménière's disease.

Long-tm treatment of Ménière's disease.


Melanogenesis: ultrastructural histochemical observations on ultraviolet irradiated human melanocytes.


Yttrium-90 in gonads of monkeys containing strontium-90.


Cerebral oligodendrogliomas. Clinical manifestations, surgical treatment and histological findings in seventy cases.


Prolongation of skin graft survival and induction of resistance to infection by phytohemagglutinin.


In vitro rosette inhibition by antihuman antilymphocyte serum. Correlation with skin graft prolongation in subhuman primates.


Testing of antihuman lymphocyte sera in chimpanzees and lower primates.


Properties of L-serine deaminase from Salmonella typhi-murium and Bacillus cereus.


Studies on swine serum immunoglobulins. II. Relationship between immunoglobulins and antibodies.


Transmission of Trypanosoma cruzi by arthropod vectors: anterior versus posterior route infection.


Immunofluorescent investigations on the swine fever virus.


Host-parasite relationship in monkeys administered live tularemia vaccine.


Relationship between atmospheric temperature and survival of airborne bacteria. Effects of temperatures ranging from -40 to 49 C on the behavior of airborne Serratia marcescens, Escherichia coli, and Bacillus subtilis var. niger were investigated. Aerosol decay rates of B. subtilis spores were not significantly affected by the temperature and remained approximately constant within the temperature range studied. The survival of airborne S. marcescens and E. coli was closely related to the temperature. An increase in temperature from -18 to 49 C resulted in a progressive increase of the biological death rate, and the relationship between the biological death rate and the temperature appeared to be linear. An increase in temperature from 24 to 49 C resulted in significantly reduced aerosol recoveries of the two vegetative organisms. At -40 C, the aerosol recovery of all three agents was consistently lower than at -18 to 24 C.


Agglutinin response of rabbits to combined Pasteurella tularensis-Brucella abortus vaccination. A schedule was developed for the simultaneous production of a Pasteurella tularensis-Brucella abortus antiserum in rabbits. Three doses of 10(8) viable P. tularensis LVS organisms were given intravenously at weekly intervals. One day prior to the final dose of P. tularensis, the rabbits received 10(9) viable cells of B. abortus strain 19 intravenously. The use of live vaccines, administered in this sequence, resulted in high agglutinin titers within 3 weeks. The maximal agglutinin titer to either organism was observed 1 week after the final injection.


Procedures for the assay of carbenicillin in body fluids. The assay of carbenicillin in clinical specimens is complicated by the fact that carbenicillin also contains a small amount of benzylpenicillin, thereby precluding the use of conventional penicillin assay organisms. This report gives details of a microbiological assay method involving the use of a strain of Pseudomonas aeruginosa which is very sensitive to carbenicillin but insensitive to benzylpenicillin. The outline of a microassay method with this organism is presented, and a method for the assay of specimens containing mixtures of carbenicillin and other antibiotics is described.


Chikungunya virus vaccine prepared by Tween-ether extraction. Chikungunya virus vaccines prepared by Tween 80 and ether inactivation of virus grown in green monkey kidney cell cultures were shown to be as immunogenic as comparable Formalin-inactivated vaccines. Both types of vaccine stimulated hemagglutination-inhibiting, complement-fixing, and neutralizing antibody and afforded protection to mice against a live virus challenge. It was shown after Tween-ether treatment of chikungunya virus that the infectivity of the virus was lost and the hemagglutinin titer was increased. By characterization of the resultant hemagglutinin by sucrose and cesium chloride density gradient centrifugation, it was found that the extracted particle was smaller in size and greater in density than the parent virus particle. Removal of lipid may account for the alterations in the physical characteristics of the infectious virus particle. Conditions for treatment of chikungunya virus with Tween and ether were found that preserved high titers of hemagglutinin as well as the immunogenicity of the virus preparations.


Spectra of susceptibility of Neisseria meningitidis to antimicrobial agents in vitro. The minimal inhibitory concentrations of 49 antimicrobial agents for 37 to 40 meningococcal strains freshly isolated from the nasopharynx of healthy carriers were determined. Coumermycin A(1) and rifampin were the most effective agents tested. The geometric mean values of the minimal inhibitory concentrations for coumermycin A(1) and rifampin were 0.0001 and 0.02 mug/ml, respectively.


Preliminary report on the development of a diffusion-in-gel method for the diagnosis of ram epididymitis. A simple gel-diffusion technique is described for the diagnosis of ram epididymitis caused by Brucella ovis. The results are shown to be very similar to those obtained by the complement-fixation test, which is currently the standard method of diagnosis. The method is suitable for use in the field and is expected to facilitate the control of ram epididymitis in areas where laboratory facilities are not available.


Plaque formation by mumps virus and inhibition by antiserum. Boston and ABC strains of mumps virus produced plaques approximately 1.0 mm in diameter in monolayers of BGM cells. The plaques were circular and either clear or target-like in form. Ricki strain virus produced plaques of similar size and form but, in addition, a red plaque was observed with this agent. The vaccine strain of mumps virus, Jeryl Lynn, produced minute clear plaques approximately 0.3 mm in diameter. Incorporation of diethylaminoethyl (DEAE)-dextran into the overlay medium did not affect the size difference between Jeryl Lynn plaques and those of the other strains. However plaques of the Jeryl Lynn and Ricki strains were more easily visualized when the overlay medium contained 400 mug/ml of DEAE-dextran. Simultaneous titration by plaque formation and roller tube infectivity showed that these two methods were of equal sensitivity. Virus neutralization by antibody was demonstrated by plaque reduction. Rise in antibody titer was observed in sera from human and animal infection, human vaccination, and rabbit immunization.


Germicidal effect of ultraviolet irradiation on paper contaminated with mycobacteria. An ultraviolet chamber for decontaminating single sheets of paper seeded with mycobacteria was investigated. The ultraviolet killing effect ranged from 50% for Mycobacterium kansasii to more than 99% for M. tuberculosis.


Short-term storage at 4 C of trypsinized tissues. Cells dispersed from tissues of certain animals remained viable for several days at 4 C although the per cent of viable cells varied among the different species.


Calcium: cofactor of transketolase from baker's yeast.


Catalytic effect of GSSG on reduction of cytochrome C by GSH - possible model for facilitation of electron transfer and energy conservation by sulfonium ion formation.


Dissociation of an autolytic enzyme-cell wall complex by treatment with unusually high concentrations of salt.


Cortisol metabolite binder. I. Identity with the dimethylaminoazobenzene binding protein of liver cytosol.


Enzyme induction in the tryptophan biosynthetic pathway in Bacillus subtilis.


Effects of lysozyme on competence for Bacillus subtilis transfection.


Interhemispheric connections of prestriate cortex in monkey.


Auditory input to frontal polysensory cortex of the squirrel monkey: possible pathways.


Smallpox vaccination by intradermal jet injection. I. Introduction, background and results of pilot studies. Jet injection has met with great success in the rapid and effective mass administration of several immunizing agents. The recent development of a jet injector nozzle specifically designed for intradermal inoculation suggested the possible extension of jet injector methodology to mass smallpox vaccination. A total of 156 volunteer subjects, 16 unvaccinated and 140 vaccinated more than 5 years previously, received either undiluted smallpox vaccine by the multiple-pressure technique, or 0.1 ml of various dilutions of smallpox vaccine by jet injector using the new nozzle. Cutaneous and serological responses in revaccinees revealed that jet injection of diluted vaccine with a titre of 10(7) TCID(50)/ml was as effective as multiple-pressure inoculation of undiluted vaccine. Among the small number of primary vaccinees, jet injection of diluted vaccine with a titre of 10(6) TCID(50)/ml appeared as effective as multiple-pressure inoculation of undiluted vaccine. No complications of vaccination occurred.The findings confirm the utility of the intradermal nozzle for jet injection of smallpox vaccine. In view of the speed of administration and the economy of vaccine, it is suggested that there is a distinct role for jet injection in global smallpox eradication efforts. Further studies on larger numbers of unvaccinated subjects and on persons with significant residual vaccinial immunity are needed to define the optimal concentration of vaccine for mass vaccination by jet injection.


An evaluation of fungal enzymes for the solubilization of wheat bran constituents.


Effects of chemicals on Fischerella muscicola (Thuret) Gom.


Carbenicillin, clinicolaboratory experience.


The influence of changes in blood volume on angina pectoris. A study of the effect of phlebotomy.


Inhibition of methotrexate resistant mutant of Streptococcus faecalis by analogs of purine and pyrimidine.


Parkinson's disease: L-dopa treatment and handwriting area.


Studies of electrosleep with active and simulated treatment.


Protrptyline in the treatment of chronic schizophrenic patients.


Molindone in anxious neurotic psychiatric outpatients: a pilot study.


Use of bufexamac for rheumatoid arthritis.


Oral antibiotic therapy of acute primary lung abscess: comparison of penicillin G and tetracycline.


Preliminary experience with vanamil, a new antacid.


Case histories of rheumatoid arthritis treated with sodium or magnesium EDTA.


Studies on the mechanism of vaccinia virus cytopathic effects: effect of inhibutors of RNA and protein synthesis on early virus-induced cell damage.


Adenoviruses types 20, 25 and 28: atypical members of group II.


Nuclear pores: the pore-annulus relationship in thin section.


Effectiveness of antibacterial soaps in a healthy population.


Infection from intravenous "scalp-vein" needles in a susceptible population.


Candida sepsis complicating parenteral feeding.


Characteristics of an attenuated rubella virus strain (Cendehill).


Long-term total parenteral nutrition. The concept of an artificial gut.


The laboratory control of viral vaccines.


The control of simian tissues used for human viral vaccine production.


Experimental study on the protective antigen derived from the vaccine strain Brucella abortus BA-19.


Properties of purified tetanus toxin and toxoid.


An attempt at using a monospecific tetanus antitoxin as a national reference preparation for flocculation tests.


Titration of diphtheria antitoxin in BSC-1 cell line culture.


Studies on the cholera toxin.

Studies on the cholera toxin.


Markers of the attenuated tick-borne encephalitis virus.


APHA conference report, 1969.

APHA conference report, 1969.


Protein and RNA inhibitors and protozoan habituation.


Automated multiphasic health testing.

Automated multiphasic health testing.


Effect of total body irradiation on the immune response of rhesus monkeys to a single antigenic stimulus.


The treatment of gonorrhea with cephaloridine intramuscularly.


"Fiberduodenoscopy"--early diagnosis of cancer of the papilla of Vater.


National environmental health programmes: their planning, organization, and administration. Report of a WHO Expert Committee.


Structure and immunogenicity of synthetic antigens.


Beta-substituted N-formyl-S-benzylcysteines.


A new spirocyclobutanone synthesis in the steroid series.


Autoantibodies in healthy subjects.

Autoantibodies in healthy subjects.


Studies on a bacteriolytic enzyme of Archangium violaceum (Myxobacteriales). II. Partial purification and properties of the enzyme.


Homocysteine-dependent transmethylases catalyzing the synthesis of methionine in germinating pea seeds.


Specific binding proteins of thyroxine and triiodothyronine in liver soluble proteins.


Isolation of a DNA ligase from phage lambda C-1-infected Escherichia coli.


Human carbonic anhydrase B: biosynthesis by reticulocytes in vitro.


Partial purification of a region from the Bacillus subtilis genome on the basis of its resistance to heat-induced strand separation.


A calculation of the purification achievable for a region of a bacterial genome on the basis of its resistance to heat-induced strand separation.


Immunologic aspects of the biogenesis of erythropoietin.


A macroglobulin with inhibitory activity against coagulation factor VIII.


Divalent cation activation of deoxycholate-solubilized and -inactivated membrane reduced nicotinamide adenine dinucleotide oxidase of Bacillus megaterium KM. After treating Bacillus megaterium KM membranes with 0.2% sodium deoxycholate, most of the membrane reduced nicotinamide adenine dinucleotide (NADH) oxidase was inactivated, and all of the membrane NADH-2,6 dichlorophenol indophenol oxidoreductase was solubilized. Dilution of the deoxycholate-treated membranes in the presence of divalent cations restored almost all of the original membrane NADH oxidase. The effectiveness of the divalent cation activation decreased in the order Ba(2+) > Ca(2+) > Mg(2+) > Mn(2+). After centrifugation, the deoxycholate-treated membranes at 100,000 x g for 1 hr, all of the NADH oxidase that was activated by a divalent cation was soluble. Cation-activated oxidase, however, was insoluble. The results show that 0.2% deoxycholate at least partially solubilizes the total electron chain from NADH to O(2) in an inactive from which can be reactivated by divalent cations with the formation of active, insoluble NADH oxidase.


Subterminal oxidation of aliphatic hydrocarbons. Evidence is presented for a catabolic pathway of n-alkane oxidation which proceeds via subterminal oxidation rather than methyl group oxidation.


Derepression of beta-lactamase (penicillinase in Bacillus cereus by peptidoglycans. In Bacillus cereus 569 a cellular inducer of beta-lactamase was isolated which has the same constituents and basic structure as the soluble peptidoglycan found in sporulation, extracts from spores, and germination extracts, and which was previously called "spore-peptide." The material has been extensively purified and characterized. Two acid-soluble, high-molecular-weight peptidoglycan fractions containing muramic acid, glucosamine, diaminopimelic acid, d-aspartate, and d- and l-alanine, -lysine, -glycine, and -glutamate, distinguishable on the basis of size and different amino acid to amino sugar ratios, have been found to be responsible for the observed induction. Both fractions are capable of inducing high levels of beta-lactamase in concentrations lower than those of benzyl penicillin required for optimal induction. Several experiments also suggest that it is the accumulation of such soluble peptidoglycan in penicillin-treated cells which leads to induction of beta-lactamase and not the penicillin itself. The "spore-peptide" inducer becomes available during sporulation, and endogenous derepression of beta-lactamase activity occurs simultaneously. Such derepression also occurs in a strain of B. cereus very sensitive to penicillin and in which both uninduced as well as "spore-peptide"-induced beta-lactamase is a small fraction of that produced by the typical penicillinase producer. These results suggest that beta-lactamase in B. cereus functions in cell wall metabolism during sporulation.


Possible involvement of beta-lactamase in sporulation in Bacillus cereus. Nonreverting beta-lactamase-negative strains were isolated from the beta-lactamase-constitutive strain, Bacillus cereus 569 H. These strains differed from both beta-lactamase-inducible and -constitutive strains not only in failure to produce beta-lactamase but also in failure to autolyze on aging, delayed sporulation, and failure to release free spores from sporangia when produced. The addition of B. cereus beta-lactamase of 15% purity to a final concentration of 10 IU/ml stimulates sporulation and particularly the release of free spores in culture from sporangia of strain 569 (inducible wild-type), 569/H (constitutive mutant of 569), and HPen(-), a nonreverting beta-lactamase strain isolated from 569/H in this laboratory. Cultures of HPen(-) did not release free spores without this treatment. Similar stimulation of sporulation and spore release by beta-lactamase from B. cereus were observed in another beta-lactamase-negative strain derived from 569/H as well as in certain sporogeny mutants of B. subtilis. The beta-lactamase preparation used in these experiments was free of peptidases, proteases, and autolysins capable of solubilizing wall from vegetative cells. These results, taken with our previous finding that a soluble peptidoglycan inducer becomes available in cultures of B. cereus only at sporulation and that normal derepression of beta-lactamase accompanies normal sporulation, suggest that beta-lactamase in B. cereus may be involved in peptidoglycan metabolism during sporulation and possibly the breakdown of sporangial wall with the concomitant release of mature spores.


Distribution of 1-aminocyclopentanecarboxylic acid in the rhesus monkey.


Experimental infection with Shigella sonnei in cynomolgus monkeys (Macaca irus).


The genetic pathology of lysosomes.

The genetic pathology of lysosomes.


Three nucleoprotein components of alfalfa mosaic virus necessary for infectivity.


The amino acid composition, antigenicity, and other characteristics of the satellite viruses of tobacco necrosis virus.


The reproductive cycle of cyanophage LPP1-G in Plectonema boryanum and its dependence on photosynthetic and respiratory systems.


Isolation of pulcherriminic acid from cultures of Bacillus cereus var. alesti.


Uterine blood flow and oxygen consumption of the pregnant uterus of the Macaca mulatta.


Method for measuring plasma diamine oxidase.


The fundamental principle of the action of the oblique ocular muscles.


Correlation of naturally occurring detachments with long-term retinal detachment in the owl monkey.


Extremity heat loss in water in humans and macaques.


The influence of vehicles on neomycin sulfate prevention of experimental ocular infection in rabbits.


The morphological basis of the underbite trait in langurs (P. melalophus and T. cristatus) with an analysis of adaptive and evolutionary implications.


Brain scanning as an aid to surgery for strokes.


The vertebral venous plexus as a major cerebral venous outflow tract.


Effects of repeated inhalation of vinyl bromide in laboratory animals with recommendations for industrial handling.


Chronic toxicity of NO2 in squirrel monkeys. 3. Effect on resistance to bacterial and viral infection.


Liver transplantation in man. The significance, patterns, and control of infection.


Electroencephalographic studies on the anticonvulsive action of intravenous diazepam.


External sphincter fatigue as an adjunct to electrical detrusor stimulation.


Renal hemodynamics and function in response to renal artery occlusion in canine and primate kidneys.


Isoniazid-resistant tuberculous superinfection.


Lactobacillus overgrowth diet as an aid in controlling Escherichia coli septicemia and endotoxemia in cancer patients: a case report.


Isolation and properties of a temperature-sensitive sporulation mutant of Bacillus subtilis. A thermosensitive sporulation mutant (t(s)-4) of Bacillus subtilis was isolated, and its morphological, physiological, and enzymatic properties were investigated. This mutant is able to grow equally well at 30 and 42 C, but is unable to sporulate at the higher temperature. Electron microscope studies have shown that the t(s)-4 mutant is blocked at stage zero of spore development. This was further confirmed by its inability to produce antibiotic when grown at the restrictive temperature and by the relatively low ribonucleic acid (RNA) and protein turnover during the stationary growth phase, characteristic for stage zero asporogenic mutants. At the permissive temperature, however, antibiotic production and RNA and protein turnover took place at the rate normally found in sporogenic strains of B. subtilis. The above properties were not altered in the parent strain when grown at either 30 or 42 C. By shifting cultures of the t(s)-4 mutant from 30 to 42 C and from 42 to 30 C at different stages of growth, we have been further able to show that the event affected at the high temperature takes place at a very early stage of spore development. As a consequence of this early block in the sporulation process, the t(s)-4 mutant grown at 42 C became defective in the late spore-specific enzymes involved in the biosynthesis of dipicolinic acid. This study suggests that the sporulation process is mediated by a regulatory protein which is altered in the thermosensitive mutant when grown at the restrictive temperature. As a result of this alteration, a pleiotropic phenotype is produced which has lost the ability to catalyze the late biochemical reactions required for spore formation.


Effect of actinomycin D on the transfer of ribonucleic acid from nucleus to cytoplasm in Lactobacillus acidophilus. After starvation for deoxyribosides, the deoxyribonucleic acid (DNA) of Lactobacillus acidophilus is restricted to a localized region of the cell. (3)H-uracil is first incorporated into such a restricted region but subsequently is found throughout the cell. This spread occurs despite the absence of protein synthesis and a major reduction in the rate of ribonucleic acid (RNA) synthesis. However, blocking RNA synthesis with actinomycin D restricts incorporation to a localized region of the cell. It is concluded that uracil is first incorporated into RNA in the bacterial nucleus from which it subsequently spreads through the cell. Actinomycin D could prevent this spread by preventing the completion of RNA molecules, which therefore do not dissociate from the DNA template.


Growth, sporulation, and enzyme defects of glucosamine mutants of Bacillus subtilis. Two glucosamine (GCA)-requiring mutants have been isolated which grow on glucose minimal or nutrient sporulation medium only in the presence of either GCA or acetyl-GCA. They lack the l-glutamine-d-fructose-6-phosphate aminotransferase (EC 2.6.1.13), which is repressible by GCA and whose activity in the standard strain decreases after cessation of growth. But the mutants can grow on GCA as sole carbon and ammonia source, because GCA induces the synthesis of 2-amino-2-deoxy-d-glucose-6-phosphate ketol-isomerase (deaminating) (EC 5.3.1.10). With respect to sporulation, the GCA-requiring mutants are in a serious dilemma, as GCA represses the onset of massive sporulation and yet a small amount of GCA-6-phosphate derivatives is necessary to allow sporulation. When GCA is continuously provided in small quantities, sporelike particles are produced which contain little or no spore cortex but a normal spore coat. Apparently, GCA derivatives are needed especially for cortex formation. Many of the sporelike particles can produce colonies after octanol, but not after heat treatment. When they are purified by treatment with lysozyme and sodium dodecylsulfate, they do not show the decrease in optical density at 600 nm typical of germination nor do they produce offspring.


Some properties of the pili of Corynebacterium renale. Some properties of the pili of the gram-positive bacteria Corynebacterium renale were described. A relationship was found between the morphological features of pili and the types of C. renale. Strains of types I and III usually possessed a small number of pili, whereas those of type II possessed numerous pili. Thick and long bundles of pili characteristic of C. renale were frequently observable in type II strains. Piliation of C. renale was stable under various cultural conditions. No ability to agglutinate red blood cells was demonstrated by piliated strains of C. renale. Pili were isolated from the cells of C. renale and studied serologically by immunodiffusion. The pili of a type II strain were serologically identical with the pili of another type II strain but not with those of the strains belonging to types I and III. The pili were serologically distinct from the cell wall. The pili were broken into short pieces by boiling, but their antigenicity was increased after boiling.


Thiostrepton, an inhibitor of 5OS ribosome subunit function. Thiostrepton inhibits (14)C-leucine incorporation by intact cells of Bacillus megaterium as well as (14)C-phenylalanine incorporation by a poly U-directed extract of Escherichia coli. Extracts of E. coli which are pretreated by incubation with thiostrepton cannot be reactivated by dialysis to more than 5% of their former activity. The 50S ribosome subunit appears to be the site of thiostrepton action, since protein-synthesizing activity can be restored to dialyzed pretreated extracts by supplementation with 50S ribosome subunits but not with 30S ribosome subunits. This technique also provides a simple sensitive method for detection of the biological activity of very small amounts of 50S ribosome subunits.


A guide to developments in caries research.