Ethanol utilization by Steptococcus faecalis.


Hemolytic behavior of sheep erythrocytes non-specifically coupled with human Cla.


Purification and properties of kanamycin-phosphorylating enzyme from Pseudomonas aeruginosa.


Cardiac transplantation in man. II. Immunosuppressive therapy.


Bovine epizootic fever. II. Physicochemical properties of the virus.


Bovine epizootic fever. 3. Loss of virus pathogenicity and immunogenicity for the calf during serial passage in various host systems.


Germination of B. anthracis spores in the peritoneal cavity of rats and establishment of anthrax.


Relationships of O antigens of Pseudomonas aeruginosa between Hungarian types of Lányi and Habs' type or Verder and Evans' types.


Isolation of tuberculin active peptides from cell wall fraction of human tubercle bacillus strain Aoyama B.


The mycology of the freeliving baboon (Papio sp.).


Experimental histoplasmosis in the baboon (Papio sp.).


Isocitric dehydrogenase activity of the cerebrospinal fluid of children with neurologic disorders.


Peripheral nerve function in pernicious anemia before and after treatment.


Effect of heparin on platelet adhesiveness.


An evaluation of nuclear arylsulfatase activity in acute leukemias.


The comparison of the coagulation factors in arterial and venous blood.


Interaction of human peripheral lymphocytes and granulocytes in the migration-inhibition reaction.


Phosphorus. From elemental light to chemical element.


Psychophysical evaluation of feedback phenomena as related to precision of force emission: some methodological considerations.


Bilateral congenital ocular defects in a foal.


Funiculosin, a new antibiotic. I. Isolation, biological and chemical properties (studies on antiviral and antitumor antibiotics. 8).


Determination of micro-quantities of haemoglobin in serum.


Persistent pupillary membrane and associated defects in the Basenji.


Diverticulum of the greater curvature of the stomach: a roentgenologic curiosity.


Blood groups of the Easter Islanders. A genetical study.


Preoperative diagnosis of common bile duct entering a duodenal diverticulum. A case report.


The angiography of hemorrhage.

The angiography of hemorrhage.


Some interesting and unusual manivestations of Crohn's disease ("regional enteritis") of the stomach, duodenum and small intestine.


Pentaerythritol trinitrate metabolism by the rat.


Diphtheria bacilli isolated in Alberta in 1967 from the throat, nose, ears and skin.


The effect of hypoxia on electrocortical activity in the cebus monkey.


Some properties of so-called anticapsular sera used in the immunofluorescent reaction with capsules of B. anthracis.


The antibacterial effect of neomycin, quaternary ammonium compounds and combinations of these substances on Pseudomonas aeruginosa.


Some manifestations of a relationship between group A and L streptococci. Preliminary report.


Endotoxins from several bacterial species as immunizing agents against Pseudomonas aeruginosa in Galleria mellonella.


Dissociation and reassociation of skeletal muscle ribosomes.


Membrane-bound ribosomes in HeLa cells. I. Their proportion to total cell ribosomes and their association with messenger RNA.


Restoration of middle cerebral artery flow in experimental infarction.


Antibodies specific for purine and pyrimidine nucleosides: effect on in vitro priming ability of DNA.


Bacterial endarteritis. A probable case in an arteriovenous fistula used for long-term hemodialysis.


Functional versus organic diagnostic problems.


Radiotherapy in malignant disease of the ovary.


Chemotherapy in the treatment of ovarian cancer.


Rapid methods of diagnosis in smallpox.

Rapid methods of diagnosis in smallpox.


The renal erythropoietic factor (REF). X. The question of species and class specificity.


Chemical characterization of endotoxic lipopolysaccharide from three strains of Pseudomonas aeruginosa.


Action of "chorionic growth hormone-prolactin" on growth and carcass composition of hypophysectomized rat.


Immunoprecipitation of diffusing antigens from single agar gel-suspended erythrocytes (cytoimmunodiffusion).


Increased host resistance to infection elicited by lipopolysaccharides from Brucella abortus.


Bacterial flora of semen and contamination of the reproductive organs of the hen following artificial insemination.


Observations on a freeze-dried prepration containing the scrapie agent.


Ophthalmia neonatorum in Glasgow.

Ophthalmia neonatorum in Glasgow.


Outstanding for the third time, a London group of technicians wins award.


The changing ideas in the design of a purpose-built practice.


Angels and ministers of state defend use. How long can the anti-fluoridationists continue?


Staff poaching--and the remedy. Simon Elliott discusses enticement and the law.


Steady the graph of income.

Steady the graph of income.


The nurse's responsibility toward the long term patient.


Parenteral hyperalimentation.

Parenteral hyperalimentation.


Metabolism of toxic substances.

Metabolism of toxic substances.


Hypoglycin and related hypoglycaemic compounds.


Thrush bowel infection: existence, incidence, prevention and treatment, particularly by a Lactobacillus acidophilus preparation.


Toxic substances and the nervous system.


Transorbital hypophysectomy in primates.


Effect of feeding on hormonal responses to 2-deoxy-D-glucose in conscious monkeys.


1,2,3,4,5,6-hexahydro-6-phenyl-2,6-methano-3-benzazocines. I. The 3-carboxamido-8-hydroxy derivative as an orally effective analgetic.


The isolation of uliginosin A and uliginosin B from Hypericum uliginosum.


Science and health care--the political problem.


Revival of personal dignity.

Revival of personal dignity.


Increasing sensitivity of immunodiffusion reactions with radioautography.


6-hydroxylated N,N-dimethyltryptamines and hallucinogenic potency.


Development of rural health services in the Northern territory.


Editorial: on the achievements of the Association.


Domicilliary care of the aged patient with mental illness.


How mental health consultant can help create a wholesome emotional climate.


The role of the nurse in public health.

The role of the nurse in public health.


10,000 years of food.

10,000 years of food.


Humans and hospitals.

Humans and hospitals.


Occupational fulfilment.

Occupational fulfilment.


Editorial: the International Council of Nurses.


Nursing in Bahrein.

Nursing in Bahrein.


Ketosis: condition critical.

Ketosis: condition critical.


Prevalence of drug resistance in patients with pulmonary tuberculosis presenting for the first time with symptoms at chest clinics in India. II. Findings in urban clinics among all patients, with or without history of previous chemotherapy.


Furazolidone (furoxone) in typhoid and para-typhoid fever.


The choice and uses of tetracycline drugs.


Use of the unanesthetized monkey in cardiovascular research.


Interactions of hormonal steroids with nucleic acids. II. Structural and thermodynamic aspects of binding.


Pepsinogens A, C, and D from the smooth dogfish.


Carbonic anhydrase isoenzymes in the erythrocytes and dorsolateral prostate of the rat. 1. Three forms of the zinc-containing enzyme carbonic anhydrase (EC 4.2.1.1) were isolated from the erythrocytes of the rat and two forms from the dorsolateral prostate of the rat. Several additional minor components were observed but not isolated. Separation of the isoenzymes was achieved by ion-exchange chromatography, polyacrylamide-gel electrophoresis and isoelectric focusing. 2. The general properties of the isolated isoenzymes, their molecular weights and their contents of zinc were closely similar. As catalysts of the hydration of carbon dioxide, however, they were distinctly different. The two most abundant isoenzymes of the erythrocytes, which were found in equal proportions, differed 70-fold in specific activity, whereas the isoenzymes of the dorsolateral prostate were similar to one another and resembled the high-activity component of the erythrocytes. The inhibition of the latter by acetazolamide (5-acetamido-1-thia-3,4-diazole-2-sulphonamide) was mainly competitive, whereas in identical conditions the low-activity erythrocyte component and the dorsolateral prostate isoenzymes were non-competitively inhibited. 3. The use of chloroform-ethanol to remove haemoglobin from the rat haemolysate was found (a) to bring about changes in the kinetic properties of the soluble isoenzymes and (b) to cause the appearance of an additional isoenzyme. 4. The actions were compared of the inhibitors acetazolamide, 1,1-dimethylaminonaphthalene-5-sulphonamide and ethoxzolamide (6-ethoxybenzothiazole-2-sulphonamide) on the hydrolysis of p-nitrophenyl acetate catalysed by the isoenzymes. 5. The low-activity erythrocyte isoenzyme was an efficient catalyst of the hydrolysis of beta-naphthyl acetate whereas the high-activity forms were much less active towards this ester. Neither of the isoenzymes present in the dorsolateral prostate catalysed this reaction. 6. Carbonic anhydrase in the rat dorsolateral prostate accounts for no more than 5% of the unusually high content of zinc in this organ.


Low-density lipoprotein accumulation in aortic and coronary artery walls.


Considerations in evaluating the zinc content of horse liver alcohol dehydrogenase preparations.


A single blind trial of Trasicor (Ciba 39,089-Ba) in angina pectoris.


Effects of nitroglycerin and propranolol on angina pectoris induced by intravenous infusion of isoproterenol.


Structure and properties of hemocyanins. IV. Dissociation of Helix pomatia hemocyanin by succinylation into functional subunits.


The localization of N6-(delta 2-isopentenyl) adenosine among the acceptor species of transfer ribonucleic acid of Lactobacillu acidophilus.


Studies on platelet proteins. 3. The identity of platelet and serum albumins.


Classification of the epilepsies. Proposal for an international classification.


Coronary arteries in young monkeys.

Coronary arteries in young monkeys.


Comments on the classification of epilepsy.


Academician Ivan Málek.

Academician Ivan Málek.


Effect of urease immunity on urease and antiurease activities in ruminants.


A modified antigen for serodiagnosis of systemic candidiasis.


American trypanosomiasis in British Honduras. V. Development of Trypanosoma (Schizotrypanum) cruzi in animals infected with strains isolated in the El Cayo District, and the occurrence of crithidia forms in the tissues.


American trypanosomiasis in British Honduras. VII. A natural infection in a wild cotton rat Sigmodon hispidus (Rodentia, Cricketidae) with a trypanosome morphologically resembling Trypanosoma sigmodoni Culbertson, 1941.


Schistosoma mansoni infection in rhesus monkeys: changes in egg production  and egg distribution in prolonged infections in intact and splenectomized monkeys.


Metabolism of S-adenosylmethionine in germinating pea seeds: turnover and possible relationships between recycling of sulfur and transmethylation reactions.


An apparently asystematic reaction of vicilin from Pisum with an antiserum to Lotus seed proteins.


Plasmodia of monkeys and malaria eradication in Brazil.


The nature of ribosomes of spores of Bacillus cereus T. and Bacillus megaterium.


Animal model of depression. I. Review of evidence: implications for research.


Equilibrium binding of adenosine diphosphate to myosin.


Identification of the amylase inhibitors from seeds of Leoti sorghum.


Penicillin-sensitive D-alanine carboxypeptidase in Anabaena variabilis, a blue-green alga.


A discussion on Trypanosoma sanmartini Garnham and González-Mugaburu, 1962.


Platelet aggregate filtration pressure--a method of measuring platelet aggregation in whole blood.


Thoracic calcifications.

Thoracic calcifications.


Immunization against Brucella infections: serological and immunological studies on a soluble antigen from Brucella melitensis.


Postepidemic surveillance studies of a food-borne epidemic of streptococcal pharyngitis at the United States Air Force Academy.


The effects of Cendehill rubella vaccine in children under unusually complicated circumstances.


Magnesium ion-dependent dissociation of polysomes and free 70 s ribosomes in Bacillus megaterium.


Inhibition of Ehrlich ascites tumor growth by nucleoprotein. II. In vivo fate of the tumor cells.


Fate of autogenous grafts, and processed heterogenous bone in the mastoid cavity of primates.


Brain swelling in the newborn rhesus monkey following prolonged partial asphyxia.


Possible graft-versus-host reaction after intrauterine transfusion for Rh erythroblastosis fetalis.


Hospital-associated infections.

Hospital-associated infections.


The reported use of medical care sources by low-income inpatients and outpatients.


Infections in pediatric surgery.

Infections in pediatric surgery.


Growting points in mammalian radiobiology and their implications for radiothrapy.


Pentachlorophenol: a discussion of its properties and its occurrence as a residue in human and animal tissues.


Nosema cuiculi: in vitro isolation.

Nosema cuiculi: in vitro isolation. A mammalian microsporidian was propagated for the first time in vitro with rabbit choroid plexus monolayer cultures. Tentative classification as Nosema cuniculi (Levaditi, 1923) was established by morphologic and tinctorial characteristics and by extrusion of sintgle polar filaments with attached sporoplasin from living spores. The method permits propagation of large numbers of organisms in putre cultutre.


Biosatellite 3. Preliminary findings.

Biosatellite 3. Preliminary findings. Physiological deterioration in the male macaque monkey flown in Biosatellite III necessitated its recall after 8.5 days of a planned 30-day flight. For the first 7 days the only telemetered signs of a progressive general decline were falling brain temperature and lowered central venous pressure, which occurred in the last 3 days of flight. Fluid loss in flight was high, caused initially by sweating and later by diuresis, and appeared to arise in redistribution of blood in visceral pools as a consequence of weightlessness. Death occurred suddenly 12 hours after the flight and was caused by ventricular fibrillation.


Continuous temperature measurements during malaria infections.


Interaction of bacterial endotoxin and liquoid with blood platelets: aggregation and decrease in the electrokinetic charge.


Internal architecture of the malaria-infected red cell, as revealed by ion-etching and scanning electron microscopy.


Immune adherence test in Chagas's disease.


Studies in complement fixing antibody in experimental Trypanosoma cruzi infection.


Some observations on xenodiagnosis with Rhodnius prolixus and Triatoma infestans in human infections with Bahian strains of Trypanosoma cruzi.


The rate of development of drug resistance in Babesia rodhaini.


The survival of Trypanosoma cruzi in the medicinal leech Hirudo medicinalis.


Virus miltiplication in human embryo intestinal organ cultures.


Myocarditis in Trypanosoma rhodesiense infections.


Experimental toxaemia in mice due to chagastoxin.


Biochemistry of mental disorders. Report of a WHO Scientific Group.


In vitro hybridization of peptidase A subunits between different human phenotypes, and between human and monkey.


Measurement of heat evolution and correlation with oxygen consumption during microbial growth.


Effect of alkylbenznes on n-alkane utilization.


The inhibition of catalase by plasma and by human tissue extracts.


An instrumental method for the detection of erythrocyte agglutination.


Phenytoin intoxication in childhood due to incorrect prescribing, dispensing and administration of suspensions.


Congenital valves of the anterior urethra.


The preparation of bovine 19S thyroglobulin by agarose-gel filtration.


Jejunal diverticulosis.

Jejunal diverticulosis.


Mechanism of non-enzymic transamination reaction between histidine and alpha-oxoglutaric acid. Non-enzymic transamination reactions at 85 degrees between various amino acids and alpha-oxoglutaric acid are catalysed by metal ions, e.g. Al(3+), Fe(2+), Cu(2+) and Fe(3+). The reaction is optimum at pH4.0. Of the 14 amino acids studied histidine is the most active. In the presence of Al(3+) histidine transaminates with alpha-oxoglutaric acid, forming glutamic acid and Al(3+)-imidazolylpyruvic acid complex as the end products. However, in the presence of Fe(2+) or Cu(2+) the products are glutamic acid and a 1:2 metal ion-imidazolylpyruvic acid chelate. The greater effectiveness of histidine in these reactions is attributed to the presence of the tertiary imidazole nitrogen atom, which is involved in the formation of stable sparingly soluble metal ion-imidazolylpyruvic acid complexes or chelates as end products of these reactions. Of the metal ions studied only Al(3+), Fe(2+), Fe(3+) and Cu(2+) are effective catalysts for the transamination reactions, and EDTA addition completely inhibits the catalytic effect of the Al(3+). Spectrophotometric evidence is presented to demonstrate the presence of metal ion complexes of Schiff bases of histidine as intermediates in the transamination reactions. These results may contribute to understanding the role of histidine in enzyme catalysis.


o-Quinones formed in plant extracts. Their reactions with amino acids and peptides. 1. The reactions of amino acids and peptides with the o-quinones produced by the enzymic oxidation of chlorogenic acid and caffeic acid have been studied manometrically and spectrophotometrically. 2. Amino acids, except lysine and cysteine, react primarily through their alpha-amino groups to give red or brown products. These reactions, which compete with the polymerization of the quinones, are followed by secondary reactions that may absorb oxygen and give products with other colours. 3. The in-amino group of lysine reacts with the o-quinones in a similar way. The thiol group of cysteine reacts with the quinones, without absorbing oxygen, giving colourless products. 4. Peptides containing cysteine react with the o-quinones through their thiol group. 5. Other peptides, such as glycyl-leucine and leucylglycine, react primarily through their alpha-amino group and the overall reaction resembles that of the N-terminal amino acid except that it is quicker. 6. With some peptides, the secondary reactions differ from those that occur between the o-quinones and the N-terminal amino acids. The colours produced from carnosine resemble those produced from histidine rather than those from beta-alanine, and the reactions of prolylalanine with o-quinones are more complex than those of proline.


Elevated serum iron, low unbound transferrin and candidiasis in acute leukemia.


Isolated defect of folic acid absorption associated with mental retardation and cerebral calcification.


Unusual manifestations of carcinoma of the nasopharynx. A case report.


The value of laparotomy and splenectomy in the staging of Hodgkin's disease.


Effect of some non-ionic surfactants on the absorption of Enduracidin from the measles.


Enzymatic hydrolysis of 125I-labeled parathyroid hormone.


Nutritional cirrhosis in rheusus monkeys: electron microscopy and histochemistry.


Cholinesterase inhibition by parathion in rhesus monkeys and the limited effect of 2-PAM.


The purification and properties of penicillin beta-lactamases mediated by transmissible R factors in Escherichia coli.


Quantitative determination of anomeric forms of sugar produced by amylases. IV. Anomeric form of maltose produced from phenyl alpha-maltoside catalyzed by saccharifying alpha-amylase from Bacillus subtilis.


Innervation ratios for large and small limb muscles in the baboon.


Further studies in the pyocine typing of Pseudomonas pyocyanea.


Direct haemagglutination by Mycoplasma pneumoniae and its inhibition by immune serum.


A note on bacteriocine typing techniques.


Differential color responses in the visual cortex of the squirrel monkey.


Opsonic capacity of type specific streptococcal antibodies.


Vaccination against plasmodia.

Vaccination against plasmodia.


The distribution of dendritic domains of retinal neurones.


American trypanosomiasis in British Honduras. VI. A natural infection with Trypanosoma (Schizotrypanum) cruzi in the opossum Didelphis marsupialis (Marsupialia, Didelphoidea), and experimental investigations of different wild-animal species as possible reservoirs for the parasite.


The therapeutic activity of some nitrofurans in experimental filariasis and trypanosomiasis.


o-Quinones formed in plant extracts. Their reaction with bovine serum albumin. 1. The reactions between chlorogenoquinone, the o-quinone formed during the oxidation of chlorogenic acid, and bovine serum albumin depend on the ratio of reactants. 2. When the serum albumin is in excess, oxygen is not absorbed and the products are colourless. This reaction probably involves the thiol group of bovine serum albumin; it does not occur with bovine serum albumin which has been treated with p-chloromercuribenzoate, iodoacetamide or Ellman's reagent. 3. When bovine serum albumin reacts with excess of chlorogenoquinone, oxygen is absorbed and the products are red. The red colour is probably formed by reaction of the lysine in-amino groups of bovine serum albumin, as it is prevented by treating the protein with formaldehyde, succinic anhydride or O-methylisourea. 4. Bovine serum albumin modified by a 1.5-fold (BSA-Q) and a fivefold (BSA-Q2) excess of chlorogenoquinone were separated by chromatography on DEAE-Sephadex A-50, and some of their properties observed. 5. Reaction of BSA-Q2 with fluorodinitrobenzene suggests that the terminal alpha-amino group, as well as lysine in-amino groups, are combined with chlorogenoquinone.


Purification and general properties of delta-aminolaevulate dehydratase from Nicotiana tabacum L. 1. delta-Aminolaevulate dehydratase (EC 4.2.1.24) was purified 80-fold from tobacco leaves and its properties were studied. 2. The enzyme had optimum pH7.4 in potassium phosphate buffer, K(m)6.25x10(-4)m at 37 degrees and pH7.4, optimum temperature 45 degrees and an activation energy of 11100 cal./mole. 3. The enzyme lost activity when prepared in the absence of cysteine, and this activity was only partly restored by the later addition of thiols. Reagents for thiol groups inactivated the enzyme. 4. Mg(2+) was essential for activity, and EDTA and Fe(2+) were inhibitory; Mn(2+) was an activator or an inhibitor depending on the concentration.


Some properties of a sporulating Bacillus subtilis mutant containing heavy DNA.


Agglutinating antigens of Lactobacillus jugurti ATCC 521.


The biochemical status of mu particles in Paramecium aurelia.


The cultivation of the rumen ciliate Entodinium simplex.


Identification of Pseudomonas aeruginosa in the clinical laboratory.


Proposed standard measure of recurrence of out-of-wedlock births to adolescents.


Availability and use of medical services in an Alaskan Eskimo community.


Some proposed "comparability areas" for U. S. statistics on cause of death.


Undergraduate program for training health planners and administrators.


Epidemiology of stroke in a rural area.

Epidemiology of stroke in a rural area.


Influence of the prevalence of infection on tuberculin skin testing programs.


Partnership for health and Medicare.

Partnership for health and Medicare.


A comparative study of the pulmonary mycoses of Canada and the United States.


Fluorescent antibody techniques for Salmonella and other enteric pathogens.


Prevalence of ascariasis and amebiasis in Cherokee Indian school children.


Status of health services in micronesia since the 1963 poliomyelitis epidemic.


An analysis of requests for help to a mental health study center.


Optimum energy in supervoltage x-ray therapy.


Effect of water on the kinetics of the postirradiation oxygen effect. ANL-7535.


Developments in fertility control. Report of a WHO Scientific Group.


Triiodothyronine uptake test in gynecologic radiation therapy.


Changes in tumor oxygen tension during radiation therapy.


Significant factors in red cell mass changes after fractionated local radiotherapy.


Neurofibromatosis.

Neurofibromatosis.


Ultrastructure of the proximal tubule of the rhesus monkey kidney.


Generalized folded skin with an underlying lipomatous nevus. "The Michelin Tire baby".


Isolation procedures affecting the retention of water-soluble nitrogen by spinach chloroplasts in aqueous media.


Serological typing of mycobacteria for tracing possible sources of avian mycobacterial infections in man. Avian mycobacteria represent a potential danger to the human population in areas where effective control of tuberculosis has been achieved, but where tuberculosis is still present in poultry. During the period 1957-67, a total of 44 cases of pulmonary and non-pulmonary disease in man caused by avian mycobacteria were recorded in Czechoslovakia. The source of infection was reliably established in only a small number of cases.The strains of bacteria isolated were, therefore, subjected to serological analysis using Schaefer's method of direct agglutination of bacterial suspensions by type-specific rabbit antisera. This procedure made it possible to differentiate true avian mycobacteria (serotypes I and II) from Runyon group III nonchromogens. The majority of the cultures isolated from man, and also a large proportion of those from cattle and swine, consisted of serotypes I and II, which are those of Mycobacterium avium.The possibility of classifying avian and atypical mycobacteria by means of agglutination procedures represents a valuable tool in the study of the epidemiology of mycobacterial diseases. Evidence was presented which indicated that, in Czechoslovakia, patients with tuberculosis due to avian mycobacteria acquire their infection mainly from animal sources.


The effect of adrenaline, noradrenaline, and propranolol on myocardial blood flow and metabolic heat production in monkeys and baboons.


Proteins in the spermathecare and haemolymph of the queen bee (Apis mellifica L. var. ligustica Spin.).


Glomerular filtration rate measurement in man by the single injection methods using 51Cr-EDTA.


Important aspects of the metabolism of the liver cell revealed in selected references.


Interferon content of organs and serum after intravenous inoculation of mice with Sindbis virus.


Cerebral blood flow measurement by external isotope detection of 131Cs.


An improved method for recording static tremors in monkeys.


A complement-fixation test for measuring Australia antigen and antibody.


Gonococcal arthritis.

Gonococcal arthritis.


Bilirubin metabolism in the fetus.

Bilirubin metabolism in the fetus. Bilirubin metabolism was studied in dog and monkey fetuses. Bilirubin-(3)H was administered to fetal animals in utero by prolonged intravenous infusion. Fetal plasma disappearance, hepatic uptake, biliary excretion, and placental transfer of bilirubin-(3)H were measured.Bilirubin metabolism and excretion in the fetus was much less efficient than in the adult. Fetal plasma levels of tritium were elevated for prolonged periods, and the combined rate of placental and fetal hepatic excretion was lower than normal values for adult hepatic excretion. Species differences were noted. Hepatic conjugation and excretion appeared to be the primary mechanism of fetal metabolism in the dog. In contrast, the amounts of conjugated bilirubin-(3)H excreted in fetal monkey bile were negligible. Small amounts of (3)H-labeled bilirubin derivatives were excreted in fetal bile, but 10 times as much of the administered material was transferred intact across the placenta and excreted by the maternal liver. The relationship of this functional difference to known anatomic and biochemical species differences is discussed. Preliminary observations on alternate routes of fetal bilirubin metabolism were obtained.


Identification of a protein fraction in the occipital cortex of the monkey rapidly labelled during the exposure of the animal to rhythmically flickering light.


Hepatozoon fusifex Sp. N., a mehogregarine from boa constrictor producing marked morphological changes in infected erythrocytes.


Natural infections and behavior of Trypanosoma rangeli and Trypanosoma cruzi in the vector Rhodnius prolixus in Colombia.


Resistance produced in mice and rats by inoculation with irradiated Trypanosoma rhodesiense.


Variations in filamentous and fibrillar organization, and associated sarcolemmal structures, in cells of the normal mammalian heart.


Gonorrhea in the obstetric and gynecologic clinic. Incidence in a voluntary hospital in an urban community.


Observations on the culture diagnosis of gonorrhea in women.


Asymptomatic gonorrhea.

Asymptomatic gonorrhea.


Comment: supervoltage radiation therapy--promise vs fulfillment.


Neurological crises following methamphetamine.


Anonymous mycobacterial osteomyelitis. A case report of a six-year-old child.


Adjuvant chemotherapy in primary treatment of breast cancer.


Metaphyseal dysostosis and the associated syndrome of pancreatic insufficiency and blood disorders.


Recovery and modification of radiation-induced division delay in developing sea urchin eggs.


Scattering foil device for high-energy electron-beam therapy.


A preliminary investigation of the effect of repeated low doses of monomethylhydrazine on operant behavior of primates. ARL-TR-69-7.


Visual performance of infra-human primates reared in an intermittently illuminated room.


The organization and administration of maternal and child health services. Fifth report of the WHO Expert Committee on Maternal and Child Health.


Malaria in mammals excluding man.

Malaria in mammals excluding man.


Anti-Gna, a new antibody reacting with a high-incidence erythrocytic antigen.


Why do bacterial protoplasts burst in hypotonic solutions?


Adenine nucleotide metabolism of blood platelets. VI. Subcellular localization of nucleotide pools with different functions in the platelet release reaction.


An analysis in vivo of histidine transfer RNA during repression and derepression in Bacillus subtilis.


Nuclear segregation without DNA replication in Bacillus subtilis.


Comparative electrophoretic studies on the protein of chloroplast and cytoplasmic ribosomes of spinach leaves.


An attempt to concentrate ribosomal RNA cistrons in Bacillus subtilis by Millipore filtration.


Adaptation of a colorimetric determination of 5-(3,3-dimethyl-1-triazeno)-imidazole-4-carboxamide (NSC-45388) and 5-[3,3-bis(2-chloroethyl)-1-triazeno]imidazole-4-carboxamide (NSC-82196) to blood.


The role of surgery in the treatment of focal epilepsy.


A new combination of hypotensive drugs (reserpine, dihydralazine, furosemide and triamterene) for the treatment of arterial hypertension without risk of hypopotassaemia.


Reappraisal of the toxicology of ethylene glycol. I. Determination of ethylene glycol in biological material by a chemical method.


Immunochemical characterization of epithelial antigen(s) reactive with pemphigus-like antibodies of rabbit and human pemphigus autoantibodies.


A selective medium with lincomycin and colistin used in isolation of N. gonorrhoeae and N. meningitidis.


Diagnostic tests and physiological relationships in thyroid disease.


Rifapicin inhibition of trachoma agent in vivo.


Change in the template specificity of RNA polymerase during sporulation of Bacillus subtilis.


Oxidation of carcinogenic azo-dyes. VII. Metabolites of some 4'-substituted derivatives of dimethyl-aminoazobenzene in the bile of rats.


Philosophy and objectives of program for residents of St. Joseph's Manor.


Absorption and effect of ingested mannoheptulose.


Influence of an oral contraceptive progestin-estrogen combination on simian uterine phosphatases. 16 adult female rhesus monkeys received combined oral quingestanol acetate and ethinyl estradiol in a 10:1 ration, 4 received steroids in a 20:1 ration.  After 6 cycles of 21 days on and 7 days off the uterine alkaline and acid phosphatases were analyzed histologically.  Dose groups included: 4 monkeys given 4 mcg per kg ethinyl estradiol and 40 mg quingestanol acetate, 4 given 20 and 200, 8 given 10 and 100, 4 given 50 and 1000.  Most monkeys were killed 8 days after the last dose.  In the 6 controls glandular phosphatases were low in early follicular phase, highest in late follicular phase, and declining in the stratum functionale in luteal phase; also acid phosphatase appeared in the stromal cells with eccentric nuclei  in luteal phase.  90% of the low and middle dose monkeys were in follicular phase and had normal menstrual cycles.  Their alkaline phosphatase was greater in stratum functionale than in stratum basale, and the acid phsophatase resembled controls in follicular phase.  Most monkeys given high doses were in luteal phase and their enzymes were correlated with cycle phase.


Immunization of rhesus monkeys against schistosome infection by cercariae exposed to high doses of x-radiation.


The present concept of the nature of hypothalamic hormones stimulating and inhibiting the release of pituitary hormones.


Eye movements from vestibular nuclei stimulation in monkeys.


Chromatographic separation of the vancomycin complex.


Antiviral properties of tetramethyldipicrylamine in vitro.


173t, a new antitumor antibiotic. I. Fermentation, purification, biological properties, and assay.


173t, a new antitumor antibiotic. II. Antitumor and toxicological studies.


Proposed model for screening antimicrobial agents for potential use in eliminating meningococci from the nasopharynx of healthy carriers.


Clinical significance of lincomycin-resistant Neisseria gonorrhoeae.


Determination of antibiotic susceptibility of Rickettsiae and Chlamydiae in BS-C-1 cell cultures.


Susceptibility of Neisseria gonorrhoeae to penicillin and tetracycline.


Toxicology and pharmacology of cephalexin in laboratory animals.


Lead intoxication in shipbuilding and shipscrapping, 1941 to 1968.


Immunochemical gel diffusion study of relationships between erythrote catalase of various species.


Transplacental passage of fetal red cells in abortion; increased incidence after curettage and effect of oxytocic drugs. In a study of early abortions (less than 16-week pregnancies) no significant increase in fetomaternal haemorrhage was found in patients having either threatened or incomplete abortions. A statistically significant increase in fetal cells in the maternal circulation, however, occurred after curettage. The administration of oxytocic drugs in conjunction with curettage in cases of incomplete abortion did not increase the incidence of transplacental passage of fetal erythrocytes when compared with curettage alone. Of the 81 patients curetted following abortion four had a feto-maternal haemorrhage of more than 0.2 ml. The largest amount of fetal blood found in the maternal circulation was 0.4 to 0.5 ml. Preliminary data evaluating the indirect Coombs test and enzyme-treated red cells in Rh-negative post-abortion cases suggest that this amount of blood is not a primary immunizing dose but a "booster" to preformed antibody.


Sulfhydryl groups in human ceruloplasmin.


Direct sensitivity test for isoniazid.

Direct sensitivity test for isoniazid.


Active principles in cholera stool.

Active principles in cholera stool.


Biophysical properties of frog virus and its deoxyribonucleic acid: fate of radioactive virus in the early stage of infection. Frog virus (FV-3) was banded by isopycnic centrifugation in cesium chloride, sucrose, or potassium tartrate. Two bands of infectivity were regularly found at positions in cesium chloride corresponding to densities of 1.26 and 1.30 g/cm(3), respectively. Deoxyribonucleic acid from either band had the following characteristics: double-stranded; a T(m) of 76.3 C in 0.1 SSC (0.015 m NaCl plus 0.015 m sodium citrate) and a buoyant density of 1.720 g/cm(3) in cesium chloride, corresponding to a guanine plus cytosine content of 56 to 58% and a molecular weight of 130 x 10(6) daltons, determined by velocity sedimentation. These data, together with electron micrographs of sections of cells infected with material from either band suggest that two types of infectious frog virus particles exists, rather than a second virus in the frog virus stocks. The composition of frog virus was determined. It was found that highly purified preparations of frog virus were composed of 55.8% protein, 30.1% deoxyribonucleic acid, and 14.2% lipid. The kinetics of adsorption and uncoating of FV-3 was studied with radioactive virus. Uncoating is comparatively rapid and in contrast to poxvirus is unaffected by inhibitors of protein synthesis.


Morphological comparison of Machupo with lymphocytic choriomeningitis virus: basis for a new taxonomic group. Striking morphologic similarities between Machupo, Tacaribe, and lymphocytic choriomeningitis viruses were found by thin-section electron microscopy. It is proposed that these viruses be brought together into a single taxonomic group.


Host-virus interaction in ribonucleic acid bacteriophage-infected Escherichia coli. I. Location of "late" MS2-specific ribonucleic acid synthesis. When actinomycin-treated, MS2-infected Escherichia coli are labeled during a brief period later than 16 min after infection, the newly synthesized MS2 ribonucleic acid (RNA) appears first in the 30,000 x g sediment, probably bound to fragments of bacterial membranes, since the radioactivity can be released from the sediment with deoxycholate or urea. With longer labeling times, radioactivity also appears in the 30,000 x g supernatant fluid. While on the membrane, the RNA is organized into particles with sedimentation coefficients of 40, 32, and 27S in the presence of low Mg(2+). In the presence of high Mg(+), MS2-specific RNA is found in polyribosomes. These data are interpreted to mean that MS2-specific RNA is synthesized and organized into larger structures on membrane. More than 8 min of labeling is required before radioactivity is found in the 81S virion which appears in the supernatant fluid.


Allotypic marker GP-gamma-2-I of guinea-pig immunoglobulins.


Action of malarial antibody in vitro.

Action of malarial antibody in vitro.


Surface ultrastructure of platelets and thrombocytes.


Influence of systemic blood pressure on blood flow and microcirculation of ischemic cerebral cortex: a failure of autoregulation.


Model studies of the circle of Willis: flow and pressure changes.


Conjugation of lactic-dehydrogenase with proteins by the use of glutaraldehyde: detection of antigens in the immunocompetent cells by means of the conjugates.


Induction of helical arrays of ribosomes by vinblastine sulfate in Escherichia coli. Physiological concentrations of vinblastine sulfate elicited ribosomal helices in large numbers in growing cultures of the osmotically sensitive mutant sud 24 and, after treatment with ethylenediaminetetraacetate, also in the K-12 strain. The helices were usually seen at the division plane and were often connected to the membrane. This method of induction of ribosomal helices offers a unique system for studying in vivo structure and function of the translational apparatus in relation to other cell components.


The surgical treatment of epilepsy.

The surgical treatment of epilepsy.


The care of the diabetic patient during emergency surgery.


Teratogenic effects of vincristine in three lines of mice.


Counting and assessing the size of bacteria with an automatic particle counter.


Subdivision of M. tuberculosis by means of bacteriophages. With special reference to epidemiological studies.


Antigenic determinants of aqueous ether extracted endotoxin from Neisseria gonorrhoeae.


Two distinct elastases from different strains of Pseudomonas aeruginosa.


Immunochemical characterization of aqueous ether extracted endotoxin from Neisseria gonorrhoeae.


Purification and mode of action of a lytic enzyme from Bacillus cereus.


New trends in the treatment of angina pectoris. Traditionally when considering the pharmacologic basis of therapy in angina pectoris, attention is focussed on alterations of coronary blood flow. Yet the diseased coronary arteries in these patients often do not appear to be capable of responding to vasodilatory drugs. Since the pain of myocardial ischemia is relieved by a number of interventions without an increase in coronary blood flow, the concept herein considered is that angina pector is best viewed as an unfavorable relation between myocardial oxygen requirements and availability. Thus, the clinical value of the major antianginal agents is thought to be based importantly upon their actions to reduce myocardial oxygen consumption rather than to increase coronary blood flow. Sublingual nitroglycerin possesses a powerful dilator effect on veins which reduces venous return and thereby the size of the heart and intra-myocardial tension; thus myocardial oxygen requirements are diminished. The beta-adrenergic receptor blocking drug, propranolol (Inderal(R)), inhibits sympathetic stimulation of the heart at rest and during exercise. Thus, myocardial oxygen requirements are diminished by the reduction in heart rate and diminished contractility. As a result of this latter action, cardiac output is reduced and thereby arterial pressure and intramyocardial tension is lowered. In patients with advanced heart disease and borderline cardiac compensation, propranolol is hazardous because it removes the availability of one of the important reserve mechanisms for maintaining cardiac compensation-the sympathetic support of the failing heart. The introduction of electrical stimulation of the carotid sinus nerves as a means of therapy in patients with angina pectoris has provided a powerful tool for the treatment of patients with refractory ischemic pain.


Lactic acidosis with recovery in diabetes mellitus on phenformin therapy.


Left heart hemodynamics during angina pectoris induced by atrial pacing.


Symptomatic treatment of cerebrovascular insufficiency with hydergine.


The influence of chlorphenesin carbamate and carisoprodol on psychological test scores.


Chemical hazards in the human environment.


Clinical study of the possible antidepressant action of NC-0687.


A controlled evaluation of haloperidol, chlorpromazine, and sodium amobarbital: intramuscular short-term use in acute psychotic patients.


Studies of the possible therapeutic interaction between cyclamate and warfarin.


Studies on the mineral nutrition of the bulbiformin producing strain of Bacillus subtilis.


Chest-wall resection for locally recurrent breast cancer.


Effect of metallic ions on the antibacterial activity of boseimycin.


The use of Serratia indica and Bacillus subtilis var. niger spores for tracing sewage dispersion in the sea.


The challenge of Gardner's syndrome.

The challenge of Gardner's syndrome.


The antagonism of enterococci on other bacteria in canned hams.


Editorial: When, oh when?

Editorial: When, oh when?


Study models in general practice.

Study models in general practice.


Address: A. C. Fones Award recipient, 1969.


Case report: oro-antral fistula of dental origin.


Presentation: the 1969 Alfred C. Fones Award.


Presentation: the 1969 Horace Hayden Award.


Oral medicine. 19. Benign and malignant tumors of the oral cavity.


Purification of rubella virus particles.


Antigenic composition of recombinant virus strains produced from human and avian influenza A viruses.


Long-stay care and its problems.

Long-stay care and its problems.


Diphenylhydantoin as an anticonvulsant: evaluation of treatment in forty mentally subnormal epileptics.


Evaluation of the results of treatment of soft tissue injury.


Factors governing the oxygen affinity of human adult and foetal blood.


Electron microscopic localization of acetylcholinesterase in the superior cervical ganglion of the rat.


Histochemical method for the demonstration of the activity of hexokinase and glucokinase.


Studies on the effect  fixation on nervous tissue. II. Changes of sizes of nuclei of nervous cells after fixaion and after further histological treatment of the nervous tissue.


Histochemical examinations of electrical injuries.


Histochemical studies on the distribution of alkaline and acid phosphatases amongst the neurons of the cerebellum, spinal and trigeminal ganglia of bat.


Studies in dumping syndrome. V. Tuberculosis in gastrectomized patients.


Roentgenologic aspects of primary Pseudomonas aeruginosa pneumonia in mechanically ventilated patients.


Ovulation.

Ovulation.


Ototoxic hearing loss and cochlear pathology in the monkey.


Amino acid stimulation of proteinase synthesis in a sporogenous Bacillus megaterium KM.


Arterial supply of the cervical spinal cord and its relation to the cervical myelopathy in spondylosis.


Active site modification of isocitrate lyase.


Cell culture bioassay for vincristine sulfate in sera from mice, rats, dogs, and monkeys.


Infection of human embryonic cell cultures with the Rauscher murine leukemia virus.


In support of ankles.

In support of ankles.


Comparison of alkaline phosphatase from human normal and leukemic leukocytes.


Cyclamates on trial: bail or custody?

Cyclamates on trial: bail or custody?


Exchange of carbon dioxide in the pregnant rhesus monkey: multicompartmental analysis of carbon dioxide kinetics. The exchange of carbon dioxide in the pregnant rhesus monkey has been studied quantitatively using sodium bicarbonate-(14)C and applying the model of a system of seven compartments. The transfer rates among the various compartments, compartment sizes, and the rate of production of carbon dioxide by fetus and mother were determined with a computer programmed to fit the theoretical model to the data by adjusting the parameter values of the model until a "best fit" was obtained. It was confirmed that the exchange of carbon dioxide between fetal and maternal blood across the placenta is rapid, that between fetal blood and amniotic fluid is slow, and that there is no appreciable exchange between maternal blood and amniotic fluid. The mean net production of CO(2) by fetus was 0.476 +/-0.0402 mmoles/kg.min, and that by mother was 0.373 +/-0.0279 mmoles/kg.min.


Hemolytic anemia with impaired hexokinase activity. Analyses of key glycolytic intermediates in freshly drawn red cells from six related individuals suggest that decreased hexokinase activity underlies the hemolytic process in the two members with overt hemolysis. Low red cell glucose 6-phosphate (G6P) was observed not only in the anemic patients but in the presumptive heterozygotes as well and served as a useful marker for the presence of the trait. Hexokinase activity was labile in distilled water hemolysates but was only slightly low when protected by glucose, mercaptoethanol, and ethylenediaminetetraacetate (EDTA). Normal red cell hexokinase was demonstrated to be dependent on glucose for maintenance of activity after heating to 45 degrees C. The cells of the proposita are unable to utilize glucose efficiently at glucose concentrations lower than 0.2 mmole/liter whereas normal cells maintain linear glucose consumption to at least 0.05 mM glucose. These qualitative abnormalities could result from the presence of a mutant hexokinase with an abnormally reactive sulfhydryl group and altered substrate affinity in the red cells of this kindred.


The effect of glucocorticoids on thyrotropin secretion. The effect of large doses of glucocorticoids on thyrotropin (TSH) secretion in normal and hypothyroid humans has been studied. Plasma TSH concentrations were measured before, during, and after treatment with dexamethasone given orally for 24-48 hr. In 17 patients with primary hypothyroidism, plasma TSH levels fell significantly during treatment to a mean of 54% of control (range 23-96%). Within 48 hr after the withdrawal of dexamethasone, TSH concentrations transiently increased above pretreatment values. The mean increase was to 156% of control (range 106-294). Similar changes, but of smaller magnitude, were observed in 15 normal subjects. Administration of single oral doses of dexamethasone and oral or intravenous doses of cortisol were followed by reduction of plasma TSH levels to 18-47% of control within 8-12 hr in eight hypothyroid patients. This fall also was followed by significant TSH rises above control values before they returned to the pretreatment levels. Mineralocorticoid administration was not followed by any changes in plasma TSH concentrations in three subjects.TSH responses to steroid were also studied in rats. In hypothyroid rats given dexamethasone intravenously, plasma TSH fell to 63% of control in 30-90 min and then returned to normal or above in 3-4 hr. Dexamethasone also reduced plasma TSH concentrations in normal rats but no rebound was observed in these animals. Dexamethasone did not block the increase in plasma TSH produced by thyrotropin releasing factor (TRF) administration in vivo. Neither basal nor TRF-mediated TSH release from hemipituitaries in vitro was reduced by dexamethasone or corticosterone. These studies indicate that glucocorticoids reduce TSH secretion and suggest that this effect occurs at a suprahypophyseal level.


Genetic anomalies of the posterior segment of the canine eye.


Immunohistochemical localizatio of protein components of catecholamine storage vesicles. 1. The distribution of specific proteins in sympathetic neurones has been examined by immunofluorescent histology using antibodies prepared against soluble protein components of the catecholamine storage vesicles of the adrenal medulla.2. Two antigen preparations were separated by ion exchange chromatography of the soluble proteins released on osmotic lysis of catecholamine storage vesicles which had been isolated by centrifugation from homogenates of sheep adrenal medulla. One fraction (AgDH) had high dopamine-beta-hydroxylase activity, while another (AgCB), consisting of the bulk of the protein, had some capacity to bind catecholamines. On disk gel electrophoresis the antigens ran as single bands with very different mobilities.3. Antisera (AsDH) and (AsCB) produced in rabbits to the two antigens were shown to react specifically with their antigens by immunodiffusion and electrophoresis in agarose.4. Indirect immunofluorescent staining of tissue sections was achieved by layering first the rabbit anti-sera, followed by goat anti-rabbit globulin serum which had been conjugated with fluorescein isothiocyanate.5. The adrenal medulla and the cell bodies of sympathetic ganglia showed the most intense green fluorescence with the immune rabbit sera, and hardly stained when pre-immune serum from the same animal was used. The reactivity of the antisera could be abolished by incubation with the corresponding antigen.6. The preterminal and terminal axons of sympathetic nerves also stained specifically but less intensely with both antisera. When the nerves were ligated for up to 24 hr, the portion immediately proximal to the constriction showed an enhanced reaction to the antisera.7. The results provide evidence that sympathetic neurones contain proteins immunologically identical to those involved in the synthesis and storage of noradrenaline in the adrenal medulla, and support the concept that granular vesicles are synthesized in the perikaryon of the neurone and are transported somatofugally in the axon.


Severe diabetic ketoacidosis.

Severe diabetic ketoacidosis.


Two hepatic cytoplasmic protein fractions, Y and Z, and their possible role in the hepatic uptake of bilirubin, sulfobromophthalein, and other anions. Two hepatic cytoplasmic protein fractions, designated Y and Z, which bind sulfobromophthalein (BSP), bilirubin, and other organic anions, have been separated by G75 Sephadex gel filtration. The physiologic role of these protein fractions has been investigated. They are present in the 110,000 g supernatant fraction from the livers of all the species tested (rats, mice, guinea pigs, Rhesus monkeys, sheep, and man). Tissues which do not preferentially extract BSP or bilirubin from plasma do not contain these fractions, with the exception of small intestinal mucosa which contains Z. Anion binding by Y and Z fractions is not due to contamination with albumin. These fractions are responsible for the cytoplasmic localization of bilirubin in Gunn rats, and the fractions bind bilirubin, BSP, or indocyanine green (ICG), whether given in vivo or added in vitro to liver supernate from normal rats. Flavaspidic acid-N-methylglucaminate, bunamiodyl, and iodipamide, drugs known to interfere with the hepatic uptake mechanism, compete with bilirubin and BSP for binding to Z. These proteins appear to be important in the transfer of organic anions from plasma into the liver and provide a tool for the investigation of hepatic uptake mechanisms.


Antibiotic prophylaxis for temporary transvenous pacemakers.


The effect of EDTA on the preferential intestinal absorption of zinc than manganese in turkey poults.


Orbital dysplasia in neurofibromatosis.

Orbital dysplasia in neurofibromatosis.


Postoperative irradiation in brease cancer. An evaluation of 291 cases treated at the University of Wisconsin Medical Center from 1955 through 1961.


The relative biological effectiveness of fast neutrons for mammalian tissues.


Cerebral vasospasm.

Cerebral vasospasm.


Plasmodium falciparum induced in the squirrel monkey, Saimiri sciureus.


Microcirculatory changes in primate liver during shock.


Antigens of rat and mouse epidermis. Immunoelectrophoretic, double gel diffusion, and immunofluorescence studies.


Effect of adenine compounds on platelet aggregation.


Zinc uptake in canine or rat spermatozoa.


Increased feeding in response to decreased glucose utilization in the rat and monkey.


Electrical polarization of thyroid follicles in the perfused rabbit thyroid gland.


Effect of glucose feeding on lipoprotein synthesis in the rat.


Effect of p-aminohippurate on renal excretion of potassium by the monkey kidney.


Primate kidney function in hemorrhagic shock.


Intravenous nutritional support of the non-alimenting patient.


Regional protection in cancer chemotherapy: an experimental study in monkeys.


Comparison of 3H-cytidine and 3H-5-uridine as precursors of RNA.


Acid ribonucleases of lysosomal and soluble fractions from rat liver.


Growth and body composition of hypophysectomized immature rats force fed by stomach tube.


Study of Rh iso-immunisation in pregnant women.


Plonephritis. 8. Bacterial interference in mixed infections in the rat kidney.


Recent trends in diphtheria in the United States.


Malaria research at the National Institute of Allergy and Infectious Diseases.


Central nervous system effects of insulin-induced hypoglycemia in the baboon (Papio papio) and monkey (Macacus mulatta).


A relationship between kidney mitochondrial structure and glutaminase activity in the acidotic rat.


The spatial selectivity of visual cells of the cat and the squirrel monkey.


The rate of the root shift in eel red cells and eel haemoglobin solutions. 1. We have measured the rate of the exchange of O(2) between eel red blood cells and their suspending fluid in a modified Hartridge-Roughton continuous-flow rapid-reaction velocity apparatus using an oxygen electrode to follow the progress of the reaction.2. The half-times for the uncomplicated oxygenation and deoxygenation reactions in red cells at 24 degrees C were approximately 0.025-0.08 sec.3. The rate of the Root shift in cell suspensions varied widely, depending on the initial condition of the CO(2)-bicarbonate buffer system in the suspending fluid, with the rate of oxygenation or deoxygenation of the intracellular haemoglobin as an upper limit.(a) The most rapid Root shift was produced by a change in extracellular P(CO2) with minimal contributions from CO(2) hydration-dehydration reactions in the suspending fluid or from ion exchanges across the membrane, and had a half-time as short as 0.040 sec.(b) The slowest Root shift was produced by an increase in the extracellular lactic acid concentration in the absence of any form of CO(2) or in the presence of acetazolamide. This process is presumed limited by the rate of H(+) or OH(-) transfer across the membrane and had a half-time in excess of 10 sec.(c) The Root off-shift produced by an increase in P(CO2) plus a decrease in extracellular pH showed no significant trend as temperature was lowered from 30 degrees to 11 degrees C.(d) The Root on-shift produced by a decrease in P(CO2) and increase in extracellular pH had a half-time of 3 sec at 30 degrees C, 9 sec at 24 degrees C and 20 sec at 11 degrees C. These changes appeared limited by the uncatalysed rate of extracellular CO(2) hydration.4. Root off- and on-shifts in cell haemolysates at 24 degrees C, produced predominantly by changing pH but with unavoidable subsequent readjustments of the CO(2)-bicarbonate buffer systems, had an initial rapid phase with half-times as low as 0.01 sec. However, the curves were not monotonic, although they became so in the presence of carbonic anhydrase, indicating partial rate limitation by CO(2) reactions.


Electrometric assay of rate of hydration of CO2 for investigation of kinetics of carbonic anhydrase.


Antidromic responses of orthodromically identified ganglion cells in monkey retina. 1. Conduction velocities of two types of on-centre monkey ganglion cells, called phasic and tonic, have been measured by stimulating their axons in the optic tract while recording from their cell bodies in the retina.2. The average conduction velocity of twenty-two phasic and twenty-seven tonic cells is 3.8+/-S.D. 0.6 and 1.8+/-S.D. 0.4 m/sec respectively. Since the latter, but not the former, show opponent-colour responses, retinal signals carrying information about colour appear to be travelling in smaller axons than those not handling such information.3. Stimulation of the optic tract elicits several graded intraretinal potentials, which are negative in the optic nerve fibre layer and positive in the inner plexiform layer. One of these potentials, which is largest near the fovea, occurs simultaneously with the antidromic impulses of tonic ganglion cells and is considered to result from extracellular current generated by these cells.4. Stimulation of the optic tract suppresses the orthodromic responses of ganglion cells, more for phasic than for tonic ones. This suppression is only observed after a cell is antidromically driven and is considered most likely due to a transient hyperpolarization of the cell's membrane potential following an impulse.


Spinal stand and stereotactic headholder.


Cold injury in man: a review of its etiology and discussion of its prediction.


Study of military blood banking and crossmatching using blood group antigens stored over five months in ACD-adenine.


Surgical research in the United States Army. Vietnam-Tokyo-Washington, D.C. (Japan, communication zone). 1.


Bone marrow examination in the treatment of metastatic breast cancer.


Hydronephrosis from shell fragment in renal pelvis.


Wilson's disease: the role of penicillamine.


Insulin resistance associated with coccidioidomycosis.


Case for diagnosis.

Case for diagnosis.


The prevalence of malarial fluorescent antibodies in Vietnam returnees with no history of overt malaria.


Medical support of studies for a sea level canal.


Combat plus twenty years. The effect of previous combat experience on psychiatric patients.


Antigenic dusts and respiratory disease.


Hypersensitivity to wood dust.

Hypersensitivity to wood dust.


Plate assay methods for amino acids. 8. Tryptophan.


A microbiological method for the determination of asparagine in blood serum.


Autopsy findings in lung cancer treated with megavoltage radiotherapy.


Field control in roentgen therapy with a 5 MeV linear accelerator by means of television.


Further evaluations of serologic test procedures used to diagnose brucellosis.


Replication of hog cholera virus in cell culture.


Serologic response in monkeys inoculated with rinderpest and measles viruses.


The effects of prostaglandins on the intraocular pressure of the rabbit. 1. The effects of intracameral injections of prostaglandins E(1), E(2), F(1a), F(2a), and A(1) were studied on the intraocular pressure (IOP) of rabbits anaesthetized with urethane.2. With the exception of prostaglandin F(1a), all the prostaglandins studied were found to be capable of producing a large, sustained rise in IOP, accompanied in many cases by miosis.3. A marked decrease in response to repeated injections was found with all the prostaglandins studied; this effect was more pronounced following a large initial response to the prostaglandin.4. The descending order of potency in their ability to raise IOP was as follows: prostaglandin E(1) approximately E(2)>F(2a)>A(1)>F(1a).5. Intracameral injections of prostaglandins E(1) and E(2) resulted in an increase in the protein content of the aqueous humour, which was related to the magnitude of the sustained increase in IOP.6. Stabilization of the blood-aqueous barrier with polyphloretin phosphate markedly reduced both the IOP response and the effect of prostaglandin E(2) on the protein content of the aqueous humour.7. It is concluded that the production of local vasodilatation and increased permeability of the blood-aqueous barrier play an important part in the effect of prostaglandins on the IOP. The involvement of prostaglandins in the response of the rabbit eye to irritation is discussed.


Hydrolysis of suxamethonium by different types of plasma. 1. A method, based on the use of the isolated frog rectus abdominis preparation, is described for studying quantitatively the hydrolysis of suxamethonium at low concentrations.2. Rates of hydrolysis of butyrylcholine, 10 mM, benzoylcholine, 0.05 mM, and suxamethonium, 0.025 mM, by plasma from different species and human plasma with genetic variants of cholinesterase were measured.3. The rates of hydrolysis of suxamethonium by different types of plasma vary widely. Human plasma with usual cholinesterase and monkey plasma hydrolyse suxamethonium more speedily than do the plasma of cats, dogs and rats, and human plasma with either atypical or fluoride resistant cholinesterase. This is only to a small extent attributable to differences in enzyme concentration and not explained by the presence of an inhibitor.4. The K(m) values for butyrylcholine are very similar for different types of plasma but the K(I) values for suxamethonium in the system plasma-butyrylcholine-suxamethonium vary greatly.5. These results and observations on the inhibition by decamethonium of the hydrolysis of butyrylcholine are consistent with the interpretation that the rates of hydrolysis of suxamethonium, 0.025 mM, obtained with different types of plasma vary because they are a function of two variables, the affinity of the ester for cholinesterase and the stability of the monosuccinyl derivative of the cholinesterase. It seems that human plasma with atypical cholinesterase hydrolyses suxamethonium much slower than does human plasma with usual cholinesterase mainly or solely because of differences in affinity of the ester for the two enzymes. On the other hand, cat plasma appears to hydrolyse suxamethonium much slower than does human plasma with usual cholinesterase mainly because the monosuccinyl derivative of cholinesterase in cat plasma is much more stable than that in human plasma. The reverse might apply for monkey plasma.6. Inhibition by dibucaine or sodium fluoride of the hydrolysis of benzoylcholine is not a general guide to rates of hydrolysis of suxamethonium by different types of plasma.


Immunochemical studies of human growth hormone, ovine prolactin, bovine growth hormone and a tryptic digest of bovine growth hormone.


Experimental chemotherapy of schistosomiasis. IV. Oogram studies with nicarbazin, an egg-suppressive agent.


Effect of salt concentration on regeneration rate in Blepharisma acclimated to high salt levels.


Spontaneous release of DNA in sequential genetic order by Bacillus subtilis.


Babesiosoma ophicephali n. sp. from the freshwater teleost Ophicephalus punctatus Bloch.


A proposed field of sensitivity on the cortex of Opalina.


The fine structure of the gregarine Lankesteria culicis parasitic in the yellow fever mosquito Aedes aegypti.


Lankesteria barretti n. sp. (Eugregarinida, Diplocystidae), a parasite of the mosquito Aedes triseriatus (Say) and a review of the genus Lankesteria Mingazzini.


Blood parasites of some reptiles of the Pacific northwest.


Pelvic dystocia secondary to urethral diverticulum and urinary retention.


Elastase in human pancreas. Immunologic and fluorescent antibody studies.


Reversible inhibition of cilia regeneration in Stentor coeruleus by isopropyl-n-phenyl carbamate.


Arterial lesions in gnotobiotic mice congenitally infected with LCM virus.


Isolation of a microtatobiote from patients with hemolytic-uremic syndrome and thrombotic thrombocytopenic purpura and from mites in the United States.


Rh sensitization after tubal pregnancy.

Rh sensitization after tubal pregnancy.


Incidence and mechanism of neonatal jaundice related to glucose-6-phosphate dehydrogenase deficiency.


Diamine oxidase in blood plasma in some vertebrates and Anodonta cygnea before and after injection of heparin.


Clinical applications of a field positioning and simulating stand.


Intracavitary thiotepa in malignant pleural and peritoneal effusions.


Cathepsin B, the lysosomal thiol proteinase of calf liver. Cathepsin B from calf liver was obtained by a method involving preparation of a lysosomal-mitochondrial pellet and treatment of this pellet with acetone. The material was extracted with an acid buffer, pH4.0, and then precipitated from the extract with acetone. The precipitate was dissolved in phosphate buffer, pH7.4, and subjected to gel filtration on Sephadex G-200 and G-100. The cathepsin B emerged in a range of molecular weight much lower than 50000 as a well-defined component. The purity of this material was checked by electrophoresis. To obtain maximum activity the enzyme had to be activated with a chelating agent and a reducing agent (i.e. EDTA and cysteine). A number of different substrates were used. The enzyme was active for the hydrolysis of both peptide bonds and ester bonds and had approximately equal reactivity in the two cases. The pH-dependence of the hydrolysis was the same with both substrates. The binding of the substrates was half-maximal at pH4.5 and at pH6.8. A thiol group occurred in the active centre but this group ought to have a much higher pK than that found in this enzyme.


Vitamin A and carotenoids. The enzymic conversion of beta-carotene into retinal in hog intestinal mucosa. The conversion of beta-carotene into retinal was studied in vitro with enzyme preparations from homogenates of hog intestinal mucosa. The hog mucosal enzyme was purified about 27-fold by precipitation with ammonium sulphate, chromatography on DEAE-Sephadex and gel filtration on Sephadex G-200. The reaction displayed a narrow optimum pH range (approx. 7.8-8.2). The enzyme was stimulated strongly by the addition of thiols, and was inhibited by thiol inhibitors and by the chelating agents alphaalpha'-bipyridyl and o-phenanthroline. The reaction required the addition of an appropriate detergent (or bile salt); maximal activity was obtained by addition of an appropriate combination of detergents and lipid (specifically Tween 40, sodium glycocholate and sphingomyelin). The reaction displayed Michaelis kinetics with K(m)1.3x10(-6)m and V(max.)1.1nmole of retinal formed/hr. (for 0.7mg. of enzyme protein). The properties of the hog enzyme are similar to those previously reported for a less purified rat enzyme preparation.


Carbonic acid production and the role of carbonic anhydrase in decarboxylation in brain. Sudden oxygenation of a thin film of rat brain homogenate, suspended between the surface of a glass pH-sensitive electrode and a gas-permeable membrane, is accompanied by a fall in pH, which is greater when carbonic anhydrase is inhibited. The result suggests that oxidative decarboxylation yields carbonic acid (HCO(3)- and H(+)), which dissociates to form molecular carbon dioxide. Brain carbonic anhydrase facilitates the formation of carbon dioxide from the decarboxylation products.


Zinc-dependent conformational stability in the alkaline phosphatase of Escherichia coli.


The effect of pancreatic ribonuclease on rabbit reticulocyte ribosomes and its interpretation in terms of ribosome structure. 1. Parts of the 16s and 30s RNA species of reticulocytes are readily hydrolysed by pancreatic ribonuclease. The biological activity of the ribosomes is diminished after treatment with low concentrations of the enzyme (e.g. 1ng. of ribonuclease/2.5mg. of polyribosome fraction/ml.). A high proportion of the chain scissions are ;hidden' owing to the secondary structure of the RNA moiety. 2. As the concentration of ribonuclease is increased RNA is lost from the ribosome. About 20-30% of the RNA may be removed from the ribosome without altering appreciably its sedimentation coefficient or its appearance in the electron microscope. 3. The amount of RNA removed from the ribosome is not increased by raising the concentration of enzyme from about 1mug. to 2.5mg. of ribonuclease/2.5mg. of polyribosome fraction/ml., or by increasing the temperature from 0 degrees to 30 degrees , or by first converting the RNA moiety into a single-stranded form before exposure to ribonuclease. 4. Untreated polyribosomes aggregate at about 75 degrees , whereas ribosomes treated with ribonuclease aggregate at about 45 degrees . The aggregates that are found on heating ribosomes after enzymic hydrolysis contain about 40-50% of the complement of RNA of intact ribosomes. 5. From the size of the fragments of RNA isolated from RNA-depleted ribosomes it is inferred that there is one site/60-100 nucleotides that is sensitive to ribonuclease. 6. The RNA moiety of RNA-depleted ribosomes has some double-helical character as shown by the optical properties and X-ray-diffraction pattern of ribonuclease-treated ribosomes and by the ;melting' properties of the isolated RNA. 7. Subparticles prepared by titration with an excess of EDTA are readily hydrolysed by ribonuclease to fragments of S(20,w) less than 4s, in contrast with the intact particle.


Drug-induced relief of the tremor in monkeys with mesencephalic lesions.


Effect of polyamines on the stability of brain-cortex ribosomes. 1. Ribosomes isolated from the cortex tissue of goat brain contain very small amounts of spermidine and spermine. Ribosomes isolated from spermidine-treated slices have a higher spermidine content. 2. The polyamines partially prevent the temperature-dependent breakdown of ribosomes into acid-soluble nucleotides. 3. The ;melting' temperature of ribosomes rises slightly when the ribosomes are heated slowly in the presence of polyamines. 4. The pH-dependent breakdown of ribosomes into protein, RNA and acid-soluble nucleotide is markedly decreased by polyamines present in media in which ribosomes are suspended. 5. The breakdown of ribosomes in the presence of high concentrations of salts and EDTA is partially checked by the concurrent presence of polyamines. 6. Spermidine and spermine make ribosomes less susceptible to enzymic digestion by crystalline trypsin and ribonuclease.


Mastocytoma and gastroduodenal ulceration. Gastric and duodenal ulcers in dogs with mastocytoma.


False aneurysm in a rhesus monkey--surgical removal and description of pathology.


The amygdala and the sense of smell.

The amygdala and the sense of smell.


Fluorimetric determination of oxidative enzymes. Analytical applications of the monoamine and diamine oxidase systems.


The effects of niridazole in experimental schistosomiasis.


Laboratory evaluation of antischistosomal agents.


Immunity in schistosomiasis.

Immunity in schistosomiasis.


Immunological studies in various types of schistosomiasis.


Use of concentrated, balanced, liquid elemental diet for nutritional management of catabolic states.


Manipulation of cortical motor responses by peripheral sensory stimulation.


Suppression of reticuloendothelial function in the rat with cyclophosphamide. Effects of cyclophosphamide immunosuppressive therapy upon reticuloendothelial function in the rat have been studied. Numerous side effects including leukopenia, hematuria, diarrhea, hemorrhagic cystitis, and petechial hemorrhage in the lungs and the small bowel were observed. Studies utilizing (32)P-labeled bacteria revealed no change in the ability of the liver, spleen, lungs, or kidneys to ingest Pseudomonas aeruginosa. Utilization of labeled Staphylococcus aureus revealed an impairment of splenic uptake in animals receiving the maximal dose of 100 mg/kg. However, the liver, lungs, and kidneys were not affected. Although no generalized defect in the ingestive powers of these organs was observed, the ability of the lungs and kidneys from treated animals to kill ingested bacteria was significantly impaired. Regardless of the bacterium employed, the lungs and kidneys revealed a decrease in bactericidal ability with increasing drug dosages. Neither the liver nor the spleen from treated animals exhibited any decrease in bactericidal effect. Possible causes of this reduction in the bactericidal ability of alveolar and renal tissue following immunosuppression with cyclophosphamide are discussed.


Immunological analysis of Mycoplasma membranes. The antigens responsible for the production of antibodies to Mycoplasma laidlawii and M. gallisepticum causing growth and metabolic inhibition of these organisms were localized in the cell membrane. Various membrane fractions were tested for serological activity. Membrane lipids were completely or almost completely inactive, whereas several preparations of defatted membrane proteins retained some serological activity, shown by their ability to stimulate metabolic inhibition antibody in rabbits and to adsorb metabolic inhibition antibody and form precipitation lines with an antiserum to the membrane. When the membranes were heated to 65 C for 1 hr, they virtually lost their ability to adsorb metabolic inhibition antibody, which suggests that the antigenic determinants are proteins. Serological activity was retained in reaggregated membranes obtained by dialysis against Mg(2+) of membranes solubilized in sodium dodecyl sulfate. The amount of solubilized membrane protein and lipid incorporated into the reaggregated membranes could be regulated by varying the Mg(2+) concentration. As the serological tests indicated that the various membrane antigens were selectively incorporated into the different reaggregated membranes, the use of controlled reaggregation of solubilized membranes is suggested as a new tool for the fractionation and antigenic analysis of membrane proteins.


Purified protoplasmic peptides of mycobacteria: in vivo and in vitro comparison of the species specificity of purified protoplasmic peptides and purified protein derivatives of mycobacterial culture filtrates. The specificity of purified protein derivatives (PPD) prepared from the culture filtrates of Mycobacterium tuberculosis (PPD), M. kansasii (PPD-Y), M. intracellulare (PPD-B), and M. scrofulaceum (PPD-G) were compared to comparable protoplasmic extracts (PPP) of the same organisms by gel diffusion and delayed hypersensitivity reactions in sensitized guinea pigs. PPD and, to a lesser degree, PPD-Y demonstrated specificities sufficient to enable identification of homologously sensitized guinea pigs in the above group of four mycobacteria. PPD-B and PPD-G did not always elicit the largest reaction in homologously sensitized animals. The PPP sensitins from M. tuberculosis and M. kansasii produced as good skin reactions at 24 and at 48 hr as did their PPD counterparts. The PPP from M. scrofulaceum and M. intracellulare were more specific and more reactive than corresponding PPD, regardless of the time of comparison. Although based on different immunological mechanisms, the specificity of these two groups of sensitins, as demonstrated by delayed hypersensitivity, correlated well with serological comparisons in the gel diffusion test. The low degree of specificity of PPD-B and PPD-G in contrast to that of corresponding PPP was reflected in the precipitin bands in agar gel.


Specificity of a bacteriolytic enzyme from Pseudomonas aeruginosa. A bacteriolytic enzyme isolated from shake-flask cultures of Pseudomonas aeruginosa and capable of lysing cells of Staphylococcus aureus was purified approximately 500-fold by passage through diethylaminoethyl cellulose and chromatography on carboxymethyl-cellulose. The purified enzyme was shown to act as an endopeptidase, cleaving the pentaglycine cross-bridges of the cell wall peptidoglycan at d-alanyl-glycine and glycyl-glycine linkages with the release of di-, tri-, and tetraglycine fragments. Release of NH(2)-alanine indicated weak N-acetylmuramyl-l-alanine amidase activity, but most of the residual peptide remained attached to the glycan. No hydrolysis of the glycan occurred. The lytic spectrum of the enzyme toward a variety of other cell walls of known peptidoglycan composition indicated relatively high specificity for peptidoglycans with polyglycine bridges.


Influence of carbon or nitrogen starvation on amino acid transport in Pseudomonas aeruginosa. Pseudomonas aeruginosa was shown to utilize the majority of commonly occurring amino acids for growth as either the sole carbon or the sole nitrogen source. During carbon or nitrogen deprivation, the rates of transport of most of the amino acids remained unchanged; however, the transport rates for glutamate, alanine, and glycine increased under these conditions and the transport rates for leucine and valine decreased. Normal transport rates for these amino acids were resumed immediately upon the addition of the required nutrient. In the absence of an external source of carbon or of nitrogen, pool amino acids underwent rapid degradation. (14)C-Amino acid pulse experiments indicated that the constitutive amino acid catabolic enzymes, normally present in the organism during growth with glucose as the carbon source, were responsible for rapid pool losses. Nutrient starvation in the presence of chloramphenicol did not prevent amino acid catabolism. This enzymic activity is interpreted as providing P. aeruginosa with a selective advantage for survival during conditions of carbon or nitrogen starvation.


Synthesis of bacterial flagella: chromosomal synchrony and flagella synthesis. Synchronous cultures of Bacillus subtilis 168 M were obtained from light-density spores germinated at 46 C and grown at 37 C. This procedure synchronizes both cell division and chromosome replication. The chromosome synchrony was demonstrated by using transformation to measure changes in marker frequency during the cell cycle. The synthesis of two enzymes and of bacterial flagellar protein was also followed. All of the proteins were found to be synthesized continuously with an abrupt doubling in the rate of synthesis at a specific time in the cell cycle. The time at which the doubling occurred for each enzyme corresponded to the time at which the structural gene for the enzyme was replicated. The doubling of the rate of flagella synthesis corresponded to the time of replication of the hisA1 gene. We conclude that the genetic locus for the factors involved in the rate-limiting steps in flagella synthesis are located on the genetic map near the hisA1 locus.


Electrophoretic and immunological differences between the cytochrome c3 of Desulfovibrio desulfuricans and that of Desulfovibrio vulgaris. The cytochrome c(3) of Desulfovibrio desulfuricans and that of D. vulgaris were purified to homogeneity as judged by disc gel electrophoresis and by ultracentrifugation. Both cytochromes had an oxidation-reduction potential of -205 +/- 5 mv at pH 7.0 and showed characteristic absorption bands at 525 and 553 nm in the reduced state. The molecular weights of the two cytochromes (calculated from sedimentation and diffusion data) were similar, with values of 13,500 to 14,300 for D. desulfuricans and 13,800 to 14,700 for D. vulgaris. The two cytochromes differed in their electrophoretic properties on Geon and polyacrylamide gel electrophoresis and did not share a common precipitating antigenic determinant as judged by immunodiffusion data.


Penetration of Pseudomonas aeruginosa by sodium chloride and its relation to the mechanism of optical effects. When cells of Pseudomonas aeruginosa were suspended in solutions containing increasing concentrations of NaCl, the optical density (OD) of the suspensions measured within 30 sec was found to increase in proportion to the increase in salt concentration. Measurement of intracellular fluid volumes indicated that the volume of the cells decreased roughly in proportion to the increase in salt concentration. After the initial increase in optical density, there was a slow decrease at all concentrations of NaCl tested except the highest, 500 mm. Metabolic inhibitors such as sodium azide and 2,4-dinitrophenol prevented the decrease. Direct analysis showed that the Na(+) and Cl(-) concentrations in the cells were 86 and 77%, respectively, of the concentrations of the ions in the suspending medium after 1 hr. Measurement of the (22)Na space in packed cells showed that Na(+) penetrated the total fluid space in the packed cells. The penetration of (22)Na was not prevented by the presence of metabolic inhibitors or by 500 mm NaCl in the suspending medium. The results indicate that the OD increases produced in suspensions of P. aeruginosa by NaCl are not due to the osmotic action of the salt. The subsequent optical density decreases observed are under metabolic control.


Chemical composition of Azotobacter vinelandii cysts. Cysts of Azotobacter vinelandii ATCC 12837 were germinated by exposure to 3.0 mm ethylenediaminetetraacetic acid (EDTA)-tris(hydroxymethyl)aminomethane buffer at pH 7.8, and their outer coats (exines) were purified by differential and isopycnic centrifugation. Electron micrographs of exine showed it to consist of multilayers of a three-membered sheet structure whose thickness was 7.0 to 7.5 nm. The inner, less electron-dense layer (intine) was also prepared from cysts by EDTA treatment, centrifugation, concentration, and dialysis. The exine consisted of 32% carbohydrate, 28% protein, 30% lipid, and 3.2% ash, with the ash comprised of 1.62% calcium, 0.02% magnesium, and 0.34% phosphorus. The amino acid composition of exine was similar to that of gram-negative bacterial cell walls. The intine consisted of 44% carbohydrate, 9.1% protein, 37% lipid, and 4.1% ash, with the ash comprised of 2.45% calcium, 0.02% magnesium, and 0.38% phosphorus. The carbohydrates of both exine and intine contained glucose, mannose, xylose, and rhamnose. Glucosamine and galactosamine were found only in the exines. The fatty acids consisted of normal, iso, and anteiso saturated fatty acids with 10 to 18 carbon atoms and mono-unsaturated C(11), C(16), and C(18) fatty acids. The exines contained mostly bound lipid, but intines contained primarily free lipid.


Environmental control of glycogen and lipid content of Mycobacterium tuberculosis. Nitrogen-limited cultures of the R(1)R(v) strain of Mycobacterium tuberculosis accumulate both glycogen and lipid as endogenous reserves. The carbon source in the medium regulates the extent of the storage material accumulation.


Relationship of footpad sensitivity to purified protein derivatives and resistance to airborne infection with Mycobacterium tuberculosis of mice vaccinated with mycobacterial cell walls. Footpads of mice sensitized by oil-treated Bacillus Calmette-Guérin (BCG) cell walls given either intravenously, subcutaneously, intradermally, intraperitoneally, or intramuscularly became swollen and reddened after injection of purified protein derivative (PPD). This reaction, greatest after intradermal and subcutaneous sensitization, generally reached a maximum about 24 hr after challenge and was still marked at 48 hr. The histological response was characterized by infiltration with both polymorphonuclear and mononuclear cells. The proportion of mononuclear cells increased with time and they predominated at 48 hr. The footpad reaction could be detected as early as 1 week after sensitization and persisted for at least 37 weeks. Footpad sensitivity to PPD and acquired resistance to airborne infection with Mycobacterium tuberculosis H37Rv were correlated in that (i) both reached a peak approximately 1 month after sensitization of the mouse, and (ii) cell walls treated with NaOH or given without oil neither protected mice against challenge infection nor sensitized them to PPD. Although, as we reported previously, mice vaccinated subcutaneously or intradermally exhibited little or no enhanced resistance to experimental infection, mice given oil-treated cell walls by these routes were highly sensitive to footpad inoculation of PPD. Therefore, the footpad test cannot be used to determine immunity of the mouse to pulmonary infection with tubercle bacilli.


Psychrophilic properties and the temperature characteristic of growth of bacteria. An attempt was made to define psycrophily on the basis of the temperature characteristic mu.


Mycobacterial polysaccharides. II. Comparison of polysaccharides from strains of four species of mycobacteria. Evidence from chemical and serological studies indicates that a cellular heteropolysaccharide, also found in lipid extracts and culture filtrate, is present as a group antigen in Mycobacterium tuberculosis H37Ra and in other strains of mycobacteria representing M. kansasii, scotochromogenic and Battey strains. Polysaccharides from the four strains contain the same main sugars, arabinose, and galactose, as revealed by thin-layer chromatography and spectrophotometric studies. In Ouchterlony gel diffusions, bands of identity are produced between the polysaccharides by using rabbit antiserum prepared against any of the four mycobacteria. Immune adsorption studies also confirm the presence of identical antigenic determinant groups. In skin tests with tuberculopolysaccharide I, a skin reaction of about equal size was elicited in guinea pigs sensitized with either M. tuberculosis H37Ra or heterologous mycobacterial antigens in Freund's incomplete adjuvant. In animals sensitized with M. tuberculosis H37Ra, skin tests with both homologous and heterologous polysaccharides elicited similar responses.


Repressible alkaline phosphatase in Neurospora crassa. 3. Enzymatic properties.


Granuloma formation induced in mice by chemically defined mycobacterial fractions. Amounts of trehalose-6,6-dimycolate as small as 1 to 5 mug can, after intravenous injection, induce in the lungs of mice formation of tubercles in which the cellular composition is indistinguishable from that in tubercles formed after an infection with living BCG bacilli. The strongest cellular response in mice was induced by cord factor from Mycobacterium kansasii; the weakest was induced by cord factor from the BCG strain of M. bovis. It was found that three intravenous injections of cord factor induced a more extensive cellular response than did one injection of the same total amount of cord factor. Mice treated intravenously with cord factor were protected against an intravenous challenge with the virulent H37Rv strain of M. tuberculosis. The cellular response in the lungs of mice to intraperitoneal injections of living BCG and cord factor was very weak compared with that after intravenous injections. Intraperitoneal vaccination of mice with cord factor did not protect the mice against a challenge with virulent tubercle bacilli. Mice vaccinated intraperitoneally with BCG were immunized although no granulomas, or very few, were present in the lungs at the time of the challenge. The significance of the cellular response induced by cord factor is discussed.


Enzyme localization during melanogenesis. The DOPA-reaction was used to identify tyrosinase in the nucleus and cytoplasm of the neural crest melanoblast of Taricha torosa, the California newt. In this urodele there is a nuclear DOPA-positive response during the normal embryonic development from the late blastula stage to the nucleus of the early melanocyte. During the gastrula stages, all nuclei of this newt are DOPA-positive. This positive nuclear response fades away after the formation of the neural crest, save in the melanoblasts. The only cells that give a positive DOPA marking in the cytoplasm are the melanoblasts. This cytoplasmic reaction appears while the melanoblast nucleus still gives a DOPA-positive reaction. Tyrosinase activity, as marked by unlabeled DOPA, has ceased in the fully mature melanocyte. The red nuclei, seen in some of the animals in the maturing melanocyte and adjacent tissues, may be in the hallachrome stage of melanin formation. There is a diffuse distribution of DOPA reactivity in the resting nucleus, and an adherence of the DOPA-marking in the region of the dividing chromosomes in the mitosis of DOPA-positive nuclei of the melanoblast. These observations suggest that tyrosinase may be among the chromosomally bound enzymes of the chromatin space.


Control of pigment production in mouse melanoma cells in vitro. Evocation and maintenance. A clonally derived amelanotic melanoma cell line repeatedly has been forced to produce pigment by the inhibitor of DNA synthesis, I-beta-D-arabinofuranosylcytosine (ara-C) at sublethal levels. One ara-C-derived melanotic line has been cloned, and has continued to produce pigment for 2 years on normal medium. The inhibitor is most effective when administered to synchronized cells in four pulses on successive days at 1.8 x 10(-5)M during the S phase of the cell cycle. Colcemid at a sublethal concentration, and growth on medium solidified with agar also evoked pigment production in this line, but a large number of other inhibitors of biosynthetic processes did not, under the conditions tested. The melanotic lines are active producers of tyrosinase (DOPA oxidase), whereas the amelanotic line produces an inhibitor of tyrosinase activity. Both enzyme and inhibitor are labile at 4 degrees C and -20 degrees C, and decay of the inhibitor in homogenates of amelanotic cells reveals a low level of residual DOPA oxidase activity. The mean population doubling time of a cloned melanotic line is 23 hr, and that of a cloned amelanotic line 16.5 hr. A similar decrease in rate of growth is found in other melanotic lines and is believed to be a significant factor in maintaining this differentiated function. Rapid growth may be related to the production of an inhibitor by the amelanotic cells.


Gas-liquid and thin-layer chromatography of phorate, disulfoton and five of their oxidation products.


Cytochemical localization of catalase in leaf microbodies (peroxisomes). Segments of mature tobacco leaves were fixed in glutaraldehyde, incubated in medium containing 3,3'-diaminobenzidine (DAB) and hydrogen peroxide, and postfixed in osmium tetroxide. Electron microscopic observation of treated tissues revealed pronounced deposition of a highly electron-opaque material in microbodies but not in other organelles. The coarsely granular reaction product is presumably osmium black formed by reaction of oxidized DAB with osmium tetroxide. Reaction of the microbodies with DAB was completely inhibited by 0.02 M 3-amino-1,2,4-triazole and was considerably reduced by 0.01 M potassium cyanide. These results, when considered in light of recent biochemical studies, strongly suggest that catalase is responsible for the reaction. Sharp localization of this enzyme in microbodies establishes that they are identical to the catalase-rich "peroxisomes" recently isolated from leaf cell homogenates. A browning reaction that occurred in leaves during the incubation step was inhibited by cyanide but not by aminotriazole and therefore could not have been caused by the same enzyme. This reaction and a slight deposition of dense material within primary and secondary walls are ascribed to oxidation of DAB by soluble and wall-localized peroxidases.


New virus-specific antigens in cells infected with influenza virus.


The course of phage phi-e infection in sporulating cells of Bacillus subtilis strain 3610.


International drug monitoring. The role of the hospital.


Mycoplasma pneumoniae infections in childhood.


Intraocular ossification and hematopoiesis.


Irritation of rabbit eye caused by contact-lens wetting solutions.


Rhizobia (with particular reference to relationships with host plants).


Sequence of bacterial reproduction.

Sequence of bacterial reproduction.


Paleomicrobiology.

Paleomicrobiology.


Interhemispheric connections of the precentral motor cortex in the rhesus monkey.


Colloid filtration as a preparative method for the radiochemical analysis of biological samples.


Organ specificity of creatine phosphokinase muscle isoenzyme.


Common antigenic specificity on the H-chains of chicken IgM and IgG.


Studies on the antigenic properties of the Fd-fragment of a human G-myeloma protein (DAW).


Perseveration of responding and nonresponding in monkeys with orbital frontal ablations.


Fluorescent TLC analysis of traces of penicillin.


Physiologic surface-active agents and drug absorption II. Comparison of the effect of sodium taurodeoxycholate and ethylenediaminetetraacetic acid on salicylamide and salicylate transfer across the everted rat small intestine.


Single-dose treatment of gonorrhea with selected antibiotic agents.


Nitrogenase activity in heterocysts of blue-green algae.


Rabies and quarantine.

Rabies and quarantine.


Why retroperitoneal lymphadenectomy for testicular tumors?


Gastrointestinal neoplasms in von Recklinghausen's disease.


Influence of hypophysial gonadotropins on the kidney of toad (Bufo melanostictus).


Studies on the effect of the histological treatment on nervous tissue. 3.


Histochemical localization of lysosomal enzymes in suprarenal bodies of elasmobranchs.


Enzymatic lysis of vegetative cells of Bacillus popilliae and other sporeformers.


Meatabolism of rutin by Polyporus versicolor PRL 572.


Dilatometric study of streptococcal growth and metabolism.


Cytotoxic activity of hemolysin from Listeria monocytogenes onL-M strain of mouse cells.


Resection of pharyngoesophageal diverticulum using stapling device.


Disc electrophoresis-immunodiffusion of serum proteins in normal human gallbladder bile.


Immunoproliferative effects of lymphocytic choriomeningitis virus in germfree mice.


Isolation of measles virus from brain cell cultures of two patients with subacute sclerosing panencephalitis.


Thyroxine-binding by muscle mitochondria from normal and genetically dystrophic chickens.


Influenza (A2-Hong Kong-68) in the baboon (Papio sp.).


Radioautography of in vitro 3H-thymidine labeled biopsies of Molluscum contagiosum.


Absorption through oral mucous membranes.


Bone lamellae.

Bone lamellae.


Some quantum aspects of the protein intermolecular interfaces in connective tissue.


Experimental ischemia in monkeys. I. Effect of ischemia on gingival epithelium.


Electrostatic field calculations of charge and dipole models of membrane systems.


Zinc in porphyria.

Zinc in porphyria.


Effect of dietary components upon zinc vailability. A review with original data.


Effect of carbenicillin on Pseudomonas aeruginosa. The activity of carbenicillin against 200 strains of Pseudomonas aeruginosa was measured by a quantitative agar dilution method. Minimal inhibitory concentrations (M.I.C.'s) for five graded inocula were measured in terms of complete inhibition (CI) and reduced growth (RG). The M.I.C. decreased progressively as inocula were reduced, median values for the 200 strains ranging from 100 to 37.5 mug. per ml. by the CI criterion, and from 75 to 25 mug. per ml. by the RG definition. Ratios of M.I.C. obtained for large and small inocula were usually small. Identical M.I.C.'s by both CI and RG criteria were most often obtained when the inoculum for the RG criterion was 1 or 2 logs higher than that for complete inhibition.Population analysis of 15 strains of Ps. aeruginosa showed that one specific drug concentration usually caused a sharp drop in proportion of viable cells, ranging from 3 to 5 logs. None of the populations were completely non-viable even at 150 mug. per ml. There was evidence that the viability of different-sized populations was reduced disproportionately by carbenicillin.Carbenicillin 300 mug. per ml. exerted appreciable bactericidal effect against nine of 15 strains of Ps. aeruginosa after a 24-hour contact period; after only six hours the bactericidal effect was very small.Quantitative sensitivity measurements for carbenicillin should include M.I.C. values for both CI and RG criteria, using a range of inocula for testing. Such M.I.C. values may well be useful in monitoring carbenicillin therapy of tissue infections.


Ultrastructure of intraorbital portion of human and monkey optic nerve.


Antigenic changes in the developing brain of the hamster.


Effects of lithium injections into the amygdala and hippocampus of awake monkeys.


Principles, rules of thumb, or ways of looking at jobs with long-term patients.


Diphenylhydantoin (Dilantin) neurotoxicity--a deceptive syndrome.


Biologic aspects of endodontics. IV. Periapical tissue reactions to root-filled teeth whose canals had been instrumented short of their apices.


The effect of trypsin digestion on the dry weights of nuclei.


Alterations of collagen structure after urea treatment.


Incidence of neonatal urinary tract infection.


The stability of phenylephrine. 3. The racemisation reaction.


A simulator for treatment planning - - physical problems.


Focal tissue necrosis.

Focal tissue necrosis.


Role of neck proprioceptors for the maintenance of dynamic bodily equilbrium in the squirrel monkey.


The influence of cryosurgery on the labyrinth of squirrel monkeys and cats.


Eustachian tuboplasty.

Eustachian tuboplasty.


Antibodies to measles antigen, control antigen, and monkey kidney antigen. Studies in patients with multiple sclerosis and other neurological diseases and normal healthy individuals.


An approximation for estimating Compton scatter from a cuboid phantom.


The relative importance of leakage and scatter in megavoltage installation design.


Reversible visual loss after proptosis from retrobulbar hemorrhage. Reproduction of the syndrome in the Cynomolgus monkey.


A new design for a feeding tube.

A new design for a feeding tube.


Fibrinogen assay by an immunodiffusion plate.


Hereditary elliptocutosis: linkage data in man.


Adenylate pyrophosphorylase: purification, reaction sequence, and inhibition by sodium ion.


Characterization of glycogens from mycobacteria.


The interaction of potassium chloride and acetate with yeast enolase.


The in vivo and in vitro induction of aryl hydrocarbon hydroxylase in mammalian cells of different species, tissues, strains, and developmental and hormonal states.


Aldolase isozyme patterns in the liver of patients with malignant tumor in the stomach or esophagus.