Biosynthesis of T1 antigen in Salmonella: biosynthesis in a cell-free system. A particulate fraction from a T1 form of Salmonella typhimurium incorporated radioactivity from uridine diphosphate (UDP)-(14)C-glucose into products associated with the particulate enzyme. A major fraction of the incorporated radioactivity was found in the cell wall lipopolysaccharide fraction. Acid hydrolysis of incorporation products produced labeled galactose, ribose, and also glucose. The incorporation of glucose could be dissociated from the incorporation of galactose and ribose under certain conditions, and was assumed to represent incorporation into a polymer not related to T1 antigen. The incorporation of galactose and ribose probably represented the synthesis of T1 side chains of lipopolysaccharide, because (i) particulate fractions from non-T1 strains incorporated much less of these sugars and (ii) periodate oxidation and borohydride reduction converted a large portion of incorporated galactose residues into arabinose. The latter finding indicates that the galactose residues are galactofuranosides substituted either at C2 or C3; about 70% of the galactose residues in T1 side chains are known to be galactofuranosides substituted at C3. UDP-(14)C-galactose preparation used was not contaminated by UDP-(14)C-galactofuranose; therefore pyranose-to-furanose conversion must have taken place at some step during the reactions described above. The mechanism of conversion of galactose to ribose is not clear, but it was not found to involve a selective elimination of C1 or C6 of galactose or glucose.


Genetics of sulfate transport by Salmonella typhimurium. Sixty-four mutants were isolated from the LT-2 wild-type strain of Salmonella typhimurium by selecting for chromate resistance. The majority of lesions were shown to lie in the cysA gene. (i) The mutants cannot take up sulfate, a finding which verifies the role of cysA in sulfate transport. In addition, 52 sulfate-transport mutants isolated without chromate selection were defective in the cysA gene. (ii) Most had less than 25% of the binding activity of the wild-type strain. (iii) Most had normal sulfite reductase (H(2)S-nicotinamide adenine dinucleotide phosphate oxidoreductase, EC 1.8.1.2) activity. (iv) Their sulfate-binding protein (binder) appears electrophoretically and immunologically normal. (v) Amber cysA mutants also make apparently normal binder in small amounts. (vi) All classical cysA mutants tested, including two with long deletions, had normal binding activity. From these observations, it is suggested that the cysA gene does not code for the binder. But many mutations in this gene reduce the binding activity in some unknown way. Other mutants, identified as cysB mutants, had neither binding nor uptake activities and their sulfite reductase activities were similarly reduced, thus confirming the regulatory role of the cysB gene. When binder was detectable, it had wild-type properties. No mutations in the binder gene were found among more than 100 mutants examined. Thus, sulfate binding has not been established as a part of sulfate transport. However, the production of binder is intimately connected with cysA, the established sulfate transport gene, and is regulated by the same mechanism that regulates both transport and the rest of the cysteine biosynthetic pathway.


Selenomethionine, a methyl donor for bacterial nucleic acids. dl-Selenomethionine is as efficient a methyl donor for nucleic acid methylation in both Bacillus megaterium KM and Escherichia coli WWU as dl-methionine.


Electron microscopy of the altered spore morphology of a ribonucleic acid polymerase mutant of Bacillus subtilis. Electron microscopy was used to analyze sporulating cells and spores of Bacillus subtilis mutants (Rif(r)) which are resistant to rifampin, an inhibitor of ribonucleic acid polymerase. The spores of Rif-18 are pleomorphic and frequently exhibit terminal knobs. These knobs first occur during late stage IV and early stage V of sporulation and are extensions of the inner and outer spore coats. Since the rifampin resistance and altered spore morphology of Rif-18 are 100% cotransformable, these data suggest that the altered spore morphology is the result of an alteration in ribonucleic acid polymerase genes. The morphology and physical dimensions are also reported for spores from Rif-11, Rif-15, and Rif-21. Significant differences in size from the wild type were observed for these mutants.


Balanced macromolecular biosynthesis in "protoplasts" of Streptococcus faecalis. Osmotically fragile forms of Streptococcus faecalis 9790 were grown in 0.5 m sucrose- or 0.5 m NH(4)Cl-stabilized medium. The "protoplast" cultures exhibit an average growth rate constant of 0.66 to 0.94 mass doublings/hr. In a variety of experiments, turbidity and the net content of protein, ribonucleic acid (RNA) and deoxyribonucleic acid increase at the same rate, indicating balanced macromolecular biosynthesis. A total of two to three mass doublings was obtained, with no evidence of cell division. After osmotic shock, "protoplast" cultures released 93 to 94% of their RNA content in a form not sedimentable at 12,800 x g for 15 min, in contrast to streptococci, which released 7% of their RNA content after the same treatment.


Heterotrophic growth of blue-gren algae in dim light. A unicellular blue-green alga, Agmenellum quadruplicatum, and a filamentous blue-green alga, Lyngbya lagerheimíi, were grown heterotrophically in dim light with glucose as major source of carbon and possibly energy. The dim-light conditions did not support autotrophic growth. The two blue-green algae appeared to have the same metabolic block, namely an incomplete tricarboxylic acid cycle, as has been found in other obligately phototrophic blue-green algae. Under dim-light conditions, glucose made a greater contribution to cell constituents (amino acids) of A. quadruplicatum and L. lagerheimii than under high-light conditions.


Amino acid-beta-naphthylamide uptake by Pseudomonas aeruginosa. Studies on the arylamidase of Pseudomonas aeruginosa indicate that the enzyme is intracellular and constitutive. However, in cells grown in a basal salts medium, the uptake of amino acid-beta-naphthylamide is induced by certain amino acids. The synthesis of protein(s) related to transport in response to exposure to amino acids is postulated since chloramphenicol completely inhibits the inducing effect of amino acids. The uptake of amino acid-beta-naphthylamide is energy dependent, and the amino acid residue is incorporated into bacterial protein.


Deoxynucleoside kinases of Bacillus megaterium KM. Dialyzed extracts of Bacillus megaterium KM contain thymidine, deoxyadenosine, and deoxyguanosine kinase activities. Thymidine kinase activity is best with deoxyadenosine triphosphate or deoxyguanosine triphosphate (dGTP) as the phosphoryl donor, whereas the best deoxyadenosine kinase activity is obtained with dGTP or adenosine triphosphate. Deoxyguanosine kinase activity functions optimally with deoxycytidine triphosphate as the donor. Although the thymidine kinase activity of crude extracts does not have a demonstrable divalent cation requirement, the addition of Mg(2+) or Mn(2+) is necessary for the formation of thymidine di- and triphosphates. The synthesis of thymidine kinase appears to be partially derepressed by thymine starvation. Incubation of extracts with deoxyadenosine and dGTP results in the substantial accumulation of deoxyadenosine di- and triphosphates. Extracts deaminate deoxycytidine to deoxyuridine, presumably as a consequence of the action of deoxycytidine deaminase, and then convert deoxyuridine to deoxyuridylic acid. B. megaterium extracts do not contain any detectable deoxycytidine kinase activity.


Mechanism of resistance to antibiotic synergism in enterococci. Enterococci exhibit two types of resistance to streptomycin. Moderately high-level resistance is observed in most naturally occurring strains and can be overcome by simultaneous exposure to penicillin. In addition, very high-level resistance is found in those strains against which penicillin plus streptomycin fail to produce synergism in vitro. To study the mechanism of streptomycin resistance in enterococci, ribosomes from a wild-type strain and from a highly streptomycin-resistant mutant were isolated, characterized, and studied in an in vitro amino acid incorporation system. The ribosomes from the organism with moderately high-level streptomycin resistance were sensitive to streptomycin in vitro, suggesting that this type of resistance is caused by failure of streptomycin to reach the ribosomes. Very high-level resistance (and lack of penicillin-streptomycin synergism), on the other hand, appears to be due to ribosomally mediated streptomycin resistance.


Genetic analysis of pleiotropic negative sporulation mutants in Bacillus subtilis. Genetic studies were undertaken on 14 pleiotropic negative sporulation mutants. These mutants (spoA) which are blocked early in the sporulation process were found to map near the terminus of the Bacillus subtilis chromosome in a region enriched in genes involved in spore formation. Two- and three-factor crosses by transduction and transformation led to the conclusion that the pleiotropic spoA mutations formed a linked cluster. The genetic distance across the cluster calculated from transformation data was compatible with the mutant sites defining a single gene. Suppressor studies revealed that either a nonsense or missense mutation in the spoA locus generated a pleiotropic negative phenotype. It was concluded that the locus codes for a protein, and the absence of this protein is responsible for the pleiotropic phenotype.


Nitrogenase activity in cell-free extracts of the blue-green alga, Anabaena cylindrica. Cell-free extracts with high nitrogenase activity were prepared by sonic oscillation and French press treatment from the blue-gree alga Anabaena cylindrica. Extracts were prepared from cells grown on a 95% N(2)-5% CO(2) gas mixture followed by a period of nitrogen starvation under an atmosphere of 95% argon-5% CO(2). No increase in the specific activity of extracts was achieved by breaking heterocysts. Activity (assayed by acetylene reduction) was found to be dependent on adenosine triphosphate (ATP), an ATP-generating system, and a low-potential reductant. Na(2)S(2)O(2) employed as reductant supports higher rates of nitrogenase activity than reduced ferredoxin. The activity is associated with a small-particle fraction that can be sedimented by ultracentrifugation. In contrast to the particulate nitrogenase of Azotobacter, which is stable in air, the A. cylindrica nitrogenase is an oxygen sensitive as nitrogenase prepared from anaerobic bacteria.


Neurofibromatosis in childhood.

Neurofibromatosis in childhood.


Effects of traumatic occlusion on the temporomandibular joint of Rhesus monkeys.


An examination of the extent of reversibility of thyroxine binding within the thyroxine distribution space in the rabbit. 1. New Zealand white rabbits were injected intraperitoneally with [(125)]L-thyroxine. Experiments were performed after 24 hr had elapsed.2. Thyroxine distribution space was calculated as 129 ml. kg(-1). Half-life of disappearance of thyroxine from the blood of the urethane anaesthetized rabbit was 653 min.3. After exchange of approximately 20 ml. kg(-1) whole blood with heparinized blood from a non-radioactive donor, equilibration of radioactivity in the recipient had produced a peak radioactivity by 2 hr.4. The thyroxine space in which complete exchange took place within 2 hr was 102.7 +/- 4.7 ml. kg(-1) in six rabbits. This is 79.6% of the thyroxine distribution space.5. These results are compared with observations in the rat.


Absorption of light by non-refractive spherical shells.


Quantitative leukocyte iodination.

Quantitative leukocyte iodination.


An epidemic of pharyngitis due to a nonhemolytic group A streptococcus at lowry air force base.


Drug-resistant tuberculosis.

Drug-resistant tuberculosis.


Visual search in monkeys.

Visual search in monkeys.


Oculodentodigital dysplasia syndrome.

Oculodentodigital dysplasia syndrome.


Development of atopic allergy to synthetic lysine vasopressin in a child suffering from Hand-Schüller-Christian disease.


Gastrointestinal aspects of cutaneous and familial diseases.


Bacteriological study of diphtheria cases. Preliminary report.


Applanation and Schiotz tonometer standardizations for the owl monkey eye with a new technique for measuring episcleral venous pressure.


Clinical, immunologic, and physiologic observations in factory workers exposed to B. subtilis enzyme dust.


The lungs as the site of delayed-type hypersensitivity reactions in guinea pigs.


The isolation of reagin and blocking antibody by means of an immunoadsorbent.


Metatarsal transplantations following unilateral mandibular condylectomies in Macaca mulatta monkeys.


Colorimetric assay of tetracycline antibiotics.


Serum levels of chloramphenicol in children, rhesus monkeys, and cats after administration of chloramphenicol palmitate suspension.


Structure-activity relationships of tetracyclines. I. Inhibition of cell division and protein and nucleic acid syntheses in Escherichia coli W.


Prolonged septicaemic salmonellosis intercurrent with Schistosomiasis mansoni (intestinal polyposis, hepatic and cardiopulmonary forms) Chagas' disease, cerebral cysticercosis, taeniasis, shigellosis, ancylostomiasis, ascariasis and chronic malnutrition. Clinopathologic discussion.


An epithelial blood-aqueous barrier to horseradish peroxidase in the ciliary processes of the vervet monkey (Cercopithecus aethiops).


Facial pain treated with carbamazepin (Tegretol).


Isolation of toxotoxin as a large molecule or particle by a method called "ice-filtration".


Effect of induced rise of blood pressure on pain in cluster headache.


Variation in staphylococcal protein A reactivity with gamma G-globulins of different species.


Utilization of thymine, thymidine and TMP by neisseria meningitidis. 1. Growth response and uptake of labelled material.


Surface immunogens of Mycobacterium tuberculosis demonstrated by immunofluorescence.


Proteolytic enzymes and biological inhibitors. 3. Naturally occurring inhibitors in some animal and plant materials and their effect upon proteolytic enzymes of various origin.


Proteolytic enzymes and biological inhibitors. IV. Bacterial proteinase inhibitors and their effect upon enzymes of various origin.


Attenuation of continuous wave laser radiation.


A tissue culture technique for the assay of antibacterial immune sera.


The iron-uptake system of Bacillus subtilis.


Factors affecting the relative ratio of fatty acids in Bacillus cereus.


Hydroxyl apatite column chromatography of enterotoxin B.


Virus-induced hemolytic anemia in mice.

Virus-induced hemolytic anemia in mice.


Assay of anti-D using the Technicon Auto-Analyzer and the international standard anti-D typing serum. A method is described for the automated quantitation of the anti-D content of sera. This procedure is far more reproducible than current manual techniques. The use of the International Standard anti-D typing serum in the calibration of a working standard enables results to be expressed in mug/ml of antibody protein.


Colistin, sulphamethoxazole, and trimethoprim in synergy against Gram-negative bacteria. The antibacterial activity of the four possible combinations of the three drugs, colistin, sulphamethoxazole, and trimethoprim, has been investigated with Gram-negative bacteria. All of the strains examined, with the exception of the strains of Proteus, were sensitive to colistin. The combination of colistin and sulphamethoxazole was synergic against all 141 sulphamethoxazole-sensitive bacteria out of a total of 164 organisms against which it was tested. The sensitive strains comprised 27 of the 37 Esch. coli, 51 of the 54 Ps. aeruginosa, 24 of the 30 Kl. aerogenes, eight of the 12 shigellae, and all 21 Proteus and 10 salmonellae tested. The combined effect was indifference against the remaining 23 organisms which were resistant to sulphamethoxazole. The combination of colistin and trimethoprim was synergic against all 72 organisms against which it was tested, which comprised 10 Esch. coli, 14 Ps. aeruginosa, 14 Kl. aerogenes, 12 Proteus spp, 10 salmonellae, and 12 shigellae. The combination of sulphamethoxazole and trimethoprim was synergic against 61 of the same 72 organisms; the exceptions were three Esch. coli, four Kl. aerogenes, and four shigellae, all of which were sulphamethoxazole resistant. The combination of all three drugs-colistin, sulphamethoxazole, and trimethoprim-was more active than combinations of any two against 66 of the 72 organisms. The exceptions were three strains of Esch. coli, two of Kl. aerogenes, and one shigella, all of which were sulphamethoxazole resistant.


Methods of dating skeletal remains.

Methods of dating skeletal remains.


Transformation of infectivity in Rhizobium japonicum.


Chromogenesis variations in pathogenic Pseudomonas aeruginosa strains evaluated by the culture media.


The oxidative metabolism of histamine in guinea-pig skin with and without beta-blockade by propanolol.


Auditory inattention.

Auditory inattention.


Selective sparing of function following prefrontal lobectomy in infant monkeys.


Fiber sorting in the fasciculus gracilis of squirrel monkeys.


Trypanosoma cruzi: immunological properties of a soluble extract of culture forms.


Immunity to malaria. II. Serological response of monkeys sensitized by drug-suppressed infection or by dead parasitized cells in Freund's complete adjuvant.


Influence of quinestrol on th histology and phosphatase activity of the simian uterus. The purpose of this study was to evaluate the endometrial acid and alkaline phosphatase activity in rhesus monkeys chronically treated for 24 months with 3 levels of quinestrol (17-ethynlestradiol cyclopentyl ether).  The 3 levels were: 1) 4 animals, 4 mcg/kg;2) 3 animals, 20mcg/kg; 3) 4 animals 100 mcg/kg.  There were 4 animals included as sesame oil controls.  All monkeys receiving quinestrol had some degree of cystic endometrial hyperplasia, dose-related, ranging from trace, to weak, to moderate cystic endometrial hyperplasia for high dose animals.  The treated monkeys also showed thickening of the glandular basement membrane and an increase in endometrial and myometrial collagen (fibrosis), also apparently dose-related.  In the monkeys receiving quinestrol, glandular alkaline phosphatase activity was comparable to that of the follicular and early luteal animals while the acid enzyme activity resembled that found in early and late luteal controls.  Frequency of menstrual cycles was reduced.


Effect of postcoital estroen on uterie carbonic anhydrase.


The enhancement of immunoprecipitation using antisera from early stages of immunization.


Suppression of developed spleen haemolytic plaque formation by neonatal antigen stimulus in mice.


Antibody production in mice. II. The mechanism of antigenic stimulation in the secondary immune response.


Acid phosphatase activity in individual neurons during chromatolysis: a quantitative histochemical study.


Metabolism of lysolecithin in vivo: effects of hyperlipemia and atherosclerosis in squirrel monkeys. We have studied the effect of long-term hyperlipemia and atherosclerosis in squirrel monkeys on the metabolism of lysolecithin-(14)C (1-palmitoyl-1'-(14)C sn-glycerol 3-phosphorylcholine) in order to explain elevated plasma and arterial concentrations of lysolecithin. The die-away curves of lysolecithin-(14)C from plasma and the timing of appearances of other (14)C-labeled moieties in plasma and other tissues demonstrated a complex pattern of metabolic reactions. There was a rapid equilibration of specific activities of lysolecithin of plasma, liver, and aortic intima plus inner media. The specific activities of lecithin peaked first in liver, then in plasma, and rose slowly in aortic intima plus inner media. The appearance of lecithin-(14)C in heart and skeletal muscle was also slower than in the liver and some other tissues. Triglycerides, and to a lesser extent, cholesteryl esters contained radioactivity. The concentrations of aortic lysolecithin in the atherosclerotic aortas were several times greater than comparable values for control aortas, and the time of equilibration of plasma and aorta lysolecithin-(14)C was much greater for the atherosclerotic group. The quantities of lysolecithin in plasma and in the pool of which the plasma was a part, were increased with hyperlipemia and atherosclerosis, as was the rate of lysolecithin production in the fast pool. Hyperlipemia was also associated with an early increase in plasma lecithin:cholesterol acyltransferase (LCAT) activity in vitro. Furthermore, nutritional hyperlipemia influenced the distribution of lysolecithin-(14)C and lecithin-(14)C between different plasma lipoproteins. The increase in concentrations of lysolecithin in the aorta occurred more slowly than that in plasma after we had induced hyperlipemia in the monkeys.


Automatic lipid extraction and thin-layer chromatography application with a prgrammed flow system. An eight-channel programmed flow system for automatic lipid extraction and TLC application is described. Each channel has a container for lipid extraction connected by Acidflex tubing through an AutoAnalyzer pump to a TLC applicator needle. Extraction containers are prepared from disposable Oxford sampler pipet tips by inserting a small cotton filter into their lower, narrower end, which is connected to the pump tubing. The applicator needles are supported vertically in a manifold, and their tips rest on a TLC plate placed on a hot plate. Serum is added to isopropanol in each extraction container, and proteins are completely precipitated in 2 min and retained in the extraction chambers by the cotton filters; lipid extracts are then transferred on to the heated TLC plate by intermittent pumping at a rate allowing for continuous evaporation of isopropanol under streams of warmed air or nitrogen. The lipids accumulate on the plate in eight small spots, one for each channel. Solvent is proportionally added into the extraction chambers from a common reservoir through Acidflex tubing in a second AutoAnalyzer pump. During the extraction procedure, both pump motors are automatically operated by a programmed timer with a solid-state switch. Of several different solvents tested, isopropanol is the fastest for protein precipitation and lipid extraction and does not extract substances from the Acidflex tubing which interfere with chromatographic separation.


The IgM antibody response in rubella during pregnancy.


The behaviour of T-mycoplasmas in tissue culture.


Serological detection of enterotoxin from food-poisoning strains of Staphylococcus aureus.


Characteristics of so-called natural antibodies in various normal sera against culture forms of Leishmania.


Babesia mephitis sp. N. (Protozoa: Piroplasmida), a hematozoan parasite of the striped skunk, Mephitis mephitis.


Anatrichosomiasis in African monkeys.

Anatrichosomiasis in African monkeys.


The "Gray" strain of Babesia microti from a human case established in laboratory animals.


An enzyme histochemical study of the effect of various concentrations of formocresol on connective tissues.


Protein composition of rabbit oviducal fluid.


Primary cerebral arterial occlusive disease in children. II. Neurocutaneous syndromes.


Experimental pantopaque arachnoiditis in the monkey.


Somatosensory system: organizational hierarchy from single units in monkey area 5. The receptive fields of single cells in area 5 of monkey parietal cortex were studied by extracellular recording. Cells were driven primarily by gentle manipulation of multiple joints residing on one or more limbs. Both excitatory and inhibitory convergence were demonstrated. It is postulated that the multijoint receptive fields of area 5 are the result of convergence from single-joint cells of the primary receiving area. An analogy is drawn between the modification of information in the visual and somatosensory systems.


Rhesus monkey vestibular cortex: a bimodal primary projection field. Single units in the rhesus monkey (Macaca mulata) cortex responded to both vestibular and proprioceptive somatosensory stimuli. This bimodal response characteristic is unlike the modality specificity noted for other primary sensory fields. The vestibular field is located, contrary to previous opinion, within a distinct cytoarchitectonic area outside of area 2.


Streptococcus viridans and Streptococcus faecalis endocarditis.


New observations on the structure and antigenicity of the M proteins of the group A Streptococcus.


Streptococcal cross-reactive antigens in relation to rheumatic fever.


Delayed-type hypersensitivity to beta-hemolytic streptococci in patients with recurrent uveitis and its therapy by means of streptococcus vaccines.


The antigenic heterogenity of M protein of Streptococcus pyogenes (type 24).


Management of acute rheumatic fever.

Management of acute rheumatic fever.


Common pitfalls in the management of streptococcal infections.


Pseudomonas aeruginosa and proteus infections in premature babies and in infants. Therapeutic trial with carbenicillin.


Evaluation of a paper strip test for the detection of niacin production by mycobacteria.


Proteins originating in the kidney in the urine of patients with hypokalemia.


Macropyroglobulinemia: immunochemical studies in three cases.


Practical aspects of betatron electron beam dosimetry.


A passive treatment verification device.


Presence of cardioactive substances in cultures of Bacillus anthracis.


Surface effects of high-energy X rays at oblique incidence.


The attenuation of 14 MeV neutrons in calcium-based concrete and the resultant production of radioactivity.


Photostimulation of nitrogen fixation in Anabaena cylindrica.


The sweat gland as a mucous gland.

The sweat gland as a mucous gland.


Antibiotic-induced alterations in the surface morphology of bacterial cells: a scanning-beam electron miscroscopy study.


Meningococcal pneumonia.

Meningococcal pneumonia.


Interaction of nucleic acids. 8. Binding of magnesium ions by nucleic acids.


Microchemical estimation of lysosomal enzymes in epithelial cells of the small intestine in storage diseases.


Lymphosarcoma of the lung with macroglobulinaemia.


Teratogenic action of aflatoxin B1, palmotoxin B0 and palmotoxin G0 on the chick embryo.


Isolation of C reactive protein and its effect on human lymphocytes in vitro.


Traumatic quadriplegia with ectopic ossification.


Possible new metabolites mediating actions of L-dopa.


Plasma cholesterol levels of monkeys maintained on various controlled and deficient diets.


Lipid metabolism during infectious illness.


Biological antioxidant protection against lipid peroxidation damage.


Carotid sinus nerve stimulation treatment of angina refractory to other surgical procedures.


Malignant external otitis.

Malignant external otitis.


Fourier analysis of left ventricular performance. Evaluation of impedance matching.


Effect of ischemia and antianginal drugs on the distribution of radioactive microspheres in the canine left ventricle.


Comparison of thioridazine tablets to chlorpromazine spansules in the maintenance care of chronic schizophrenics.


SCH 12041. A new antianxiety agent.

SCH 12041. A new antianxiety agent.


Clinical experience with thioridazine (Melleril) in the treatment of anxiety and depression associated with emotional disorders in general practice.


Accuracy of an automated ultrasound blood pressure monitor.


Circulatory effects of intravenous pentazocine in patients with acute myocardial infarction.


Intramuscular, intra-articular and systemic tolerance of methylprednisolone acetate.


Purification and characterization of a mouse mast cell esteroprotease.


A 4-pi liquid-scintillation whole-body counter. II. Coincidence counting studies.


Preparation of tritiated thyrotropin releasing hormone (TRH) by the Wilzbach method.


Sensitivity to proteolytic enzymes in laundry detergents.


The effect of dietary lactobacilli on in-vitro catabolic activities of the small-intestinal microflora of newly weaned pigs.


Soluble antigens of enteropathogenic Escherichia coli.


Distribution of systemic blood flow of the rhesus monkey during cardiopulmonry bypass.


Chemical and immunochemical properties of two classes of globoside from equine organs.


A case of Fe-fragment disease.

A case of Fe-fragment disease.


Production of antibody against Pseudomonas aeruginosa and its serological typing.


Immunogenicity in monkeys of a combined toxoid from the main toxic principles separated from Habu snake venom.


Serological comparisons of feline respiratory viruses.


A comparative electron microscopic study on the morphogenesis of canine distemper and rinderpest viruses.


Results of treatment of the nephrotic syndrome in children in the light of the author's observations.


Isolation of tubercle bacilli from the sputum after treatment with Teepol XL, sodium lauryl sulfate, Sterinol or Cetavlon.


Late clinical and functional results in patients after pneumonectomy because of tuberculosis. I. Analysis of the epidemiological and clinical condition in patients before and after pneumonectomy.


Mutagenic action of edeine in bacteria. I. Estimation of the minimal mutagenic concentration of antibiotic.


Pregnancy outcome following oral contraceptives.


Sensitivity and reproducibility of Thayer-Martin culture mddium in diagnosing gonorrhea in women.


Lymphocyte reaction in mixed wife-husband leukocyte cultures in relation to infertility.


Diagnosis of canine babesiasis by a gel precipitation test.


The connections of the intra-osseous segment of the facial nerve in baboon (Papio sp.).


On the mechanism of modulating the volume of the voice in howling monkeys.


Response to intravenous glucose in juvenile macaque monkeys. Effect of central nervous system lesions and species difference.


Plasma and pancreatic insulin concentrations in adult squirrel and rhesus monkeys.


Detection of Australia antigen in human viral hepatitis.


Primary drug resistance in tuberculosis.


Anterior segment chemical sympathectomy by 6-hydroxy-dopamine. I. Effect on intraocular pressure and facility of outflow.


"Ischemia" during angina pectoris.

"Ischemia" during angina pectoris.


Menstrual synchorony and suppression.

Menstrual synchorony and suppression.


Visna virus: a slow virus with an RNA dependent DNA polymerase.


Investigations on urinary estrogen excretion from ewes with special reference to the cliniclel condition of the genital tract. I. The estrous cycle.


Progesterone levels in the bovine peripheral plasma measured by the competitive protein binding technique.


Metastatic bone neoplasms in dogs, cats and a lion (with some comparative remarks on the situation in man).


Hydroxyproline excretion in iron deficiency anaemia prior to and after substitution with iron-dextran.


Glutathione concentration and stability of the red blood cells in acute childhood leukemia.


Aneuploidy in paroxysmal nocturnal haemoglobinuria.


Collimator and scattering foil for 10-20 MeV electrons.


Intercomparisons of absorbed dose determinations in 10 to 35 MeV electron radiation.


Bisalbuminemia of the fast type. Observations about a new family.


The innocuousness of amniotic fluid infusion in the pregnant rhesus monkey.


Chromosome activity and cell function in polytenic cells. IV. Polyribosomes and their sensitivity to actinomycin.


Increase in acid-stable ribonuclease activity in fasted rat liver.


Effects of Mycobacterium tuberculosis on HeLa cell culture.


Serum neutralization of Kyasanur Forest disease virus in mice.


Studies of validamycins, new antibiotics. 3. Bioassay methods for the determination of validamycin.


A aturally acquired ifection of Plasmodium brasilianum in the marmset, Saguinus geoffroyi.


Transplantation antigens in rhesus monkeys.


The effect of a milk diet upon Plasmodium berghei, Nuttallia (=Babesia) rodhaini and Trypanosoma brucei infections in mice.


Malarial S-antigens from man and owl monkey infected with Plasmodium falciparum.


Naso-ocular clefts.

Naso-ocular clefts.


Stimulus control after intradimensional discrimination training.


Studies on cortical necrosis in rabbit renal homografts.


The role of hemoglobin affinity for oxygen and red-cell 2,3-diphosphoglycerate in the management of diabetic ketoacidosis.


Technical aspects of radial immunodiffusion and electroimmunodiffusion.


"Last minute" blood grouping and Rh typing.


Human brain sialidase.

Human brain sialidase.


Streptococcus faecalis aspartate transcarbamylase. I. Purification and general properties.


Early feeding of premature infants. Effect on blood sugar and gross motor activity.


Differential diagnosis of Burkitt's lymphoma of the face and jaws.


Studies on the division cycle of mammalian cells. IV. Incorporation of labeled precursors into DNA of synchronously dividing cells in culture.


Multiplicity and specificity of UDP-glucuronyltransferase. I. Effect of divalent cations and EDTA on the activity of UDP-glucuronyltransferase assayed with bilirubin, 4-methylumbelliferone and p-nitrophenol.


Rapid detection of mycoplasma-infected cell cultures.


Regulation of DNA synthesis in mouse macrophages. I. Sources, action and purification of the macrophage growing factor (MGF).


Microfilaments from amoeba proteins.

Microfilaments from amoeba proteins.


The detection of Australia antigen and anti-Au antibodies by a rapid procedure combining electrophoresis and immunoprecipitation.


Parenteral hyperalimentation in complicated surgical problems.


Hb Sogn (beta 14 arginine). Haematological and genetical studies.


Frequency of the Australia-SH antigen and antibody among Norwegian blood donors.


Comparative studies of fructose 1,6-diphosphate aldolase from Escherichia coli 518 and Lactobacillus casei var. rhamnosus ATCC 7469.


Fine structure and fatty acid composition of a motile streptococcus.


Comparison of various methods for preparation of viral serological antigens from infected cell cultures. In efforts to prepare more potent and sensitive viral serological antigens, several aspects of the production of antigens from infected cell cultures were studied. Antigens derived from whole, infected culture material and from the cellular and fluid phases were compared. Freezing and thawing, sonication, and alkaline buffer extraction were compared for effectiveness in releasing antigen from host cells. The effect of the multiplicity of infection on titers of viral antigens produced in cell cultures was studied. Generally, higher titered antigens were derived from the infected cells than from the culture fluids, but for certain viruses complement-fixing (CF) antigens derived from the culture fluids gave higher antibody titers than did cell-associated antigens. With each virus-host cell system studied, treatment with alkaline buffers extracted appreciable amounts of CF antigen from the host cells, but in some instances more antigen was released by freezing and thawing or by sonication. Extraction of infected cells with alkaline buffers was not a satisfactory method for preparation of hemagglutinating (HA) antigens for any of the viruses studied. The highest-titered HA antigens were produced from infected cells disrupted by freezing and thawing or sonication. The highest titered CF and HA antigens were produced from cell cultures infected at multiplicities of one or greater. Complement-fixing antigens produced by infecting cells in suspension and then planting had lower titers than antigens produced in parallel by infecting developed monolayers. Optimal methods are summarized for preparation of serological antigens to a variety of viruses of man.


Comparison of the pulmonary bactericidal capacity of mice and rats aganist strains of Pseudomonas aeruginosa. After aerosol deposition of Pseudomonas aeruginosa strains in mice and rats, completely different patterns of pulmonary bacterial clearance were observed in each animal species.


Combined action of sulfamethoxazole, trimethoprim, and ethylenediaminetetraacetic acid on Pseudomonas aeruginosa. Potentiation of the sulfamethoxazole-trimethoprim combination by ethylene-diaminetetraacetic acid inhibited, in vitro, four strains of Pseudomonas aeruginosa which were resistant to the sulfamethoxazole-trimethoprim combination alone.


Studies of the mechanism of action of the antitumor agent 5(4)-(3,3-dimethyl-1-triazeno) imidazole-4(5)-carboxamide in Bacillus subtilis.


Synaptic vesicles. Incorporation of choline by isolated synaptosomes and synaptic vesicles.


Changes in primate social behavior after treatment with alpha-methyl-para-tyrosine.


Primary amyloidosis iwith plasmacytic dyscrasia and a tetramer of Bence Jones type lambda globulin in the serum and urine.


Isolation of a basic protein antigen of low ragweed pollen.


Plasma protein behavior during training in untrained subjects.


The simultaneous measurement of 51Cr and 14C by liquid scintillation counting.


Experimental ovarian vein dilation.

Experimental ovarian vein dilation.


Cervical pharyngostomy for tube feeding.


Bicarbonate for juvenile diabetic coma.

Bicarbonate for juvenile diabetic coma.


Effect of tolbutamide on blood pressure.


Intracisternal granules in endothelial cells of human pathologic glomeruli.


The effect of sodium aurothiomalate ("Myocrisin") on multivesicular body formation in cultured sheep lymphocytes.


Observations of the anterior pituitary cytology in the phenobarbital treated rat.


An electron microscopic study of the small intestine in human cholera.


Effects of magnesium-deficient diet on the thyroid C cells and parathyroid gland of the dog.


Pavlovian differentiation of heart rate in the rhesus monkey.


Electroprecipitin test for hepatitis--associated antigen and antibody.


Hepatitis-associated antigen: improved sensitivity in detection.


Rubella hemagglutination-inhibition test using erythrocytes from albino pigeons.


Depression in primates.

Depression in primates.


Isolation and properties of polysomes from human placenta.


Circular dichroism of Escherichia coli ribosomes: effect of heating and metal ions.


Coordinate synthesis of ribosomal RNA and messenger for ribosomal proteins.


Experimental determination of the molar differential extinction coefficient of P700.


Increased content of cytochromes 554 and 562 in Anabaena variabilis cells grown in the presence of diphenylamine.


The intramolecular heterogeneity of Bacillus subtilis DNA studied by isopycnic CsC1 gradient.


Intra-articular Thiotepa in rheumatoid disease. A clinical analysis of 123 injected MP and PIP joints.


Antinuclear antibodies, rheumatoid factor and C-reactive protein in serum of normal women using oral contraceptives. To assess the effect of oral contraceptives on antinuclear antibodies (ANAs), rheumatoid factor (RF), and C-reactive protein (CRP), 3 groups of women were studied: 1) 174 women (ages 16-47) from the time of their initial uses of oral  contraceptives, 2) 119 women (ages 17-39) selected from the community at random, and 3) 210 women (ages 17-47) who had been taking oral contraceptives prior to and continuing through the course of this study.  Data from the original 174 were available for only 82 of the subjects.  4 women from this group of 82 with negative ANA tests prior to treatment developed positive ANAs (all took oral contraceptive combinations containing 80 mcg mestranol per tablet), 9 developed RF, and 30 developed CRP in less than a year.  The prevalence of positive tests for ANA, RF, and CRP was greater in the initial group of 210 women who had been using oral contraceptives than that found in the initial group of 174 women who had not used oral contraceptives until the study.  Cross-comparison of the 3 groups demonstrated no significant difference in the prevalence of positive tests for ANA.  Data suggest that frequency of positive tests for ANA increases with chronological age.  When group comparisons were made only the increase in CRP was statistically significant (p less than .05).  Composition and dosage of oral contraceptives may have influenced the induction of antinuclear antibodies in the group of 82 women examined from initial use of oral contraceptives.


Ambulatory treatment of pulmonary tuberculosis with ethambutol-isoniazid. Cooperative study conducted in Argentina, Colombia, Ecuador and Paraguay.


Tuberculosis in Iran.

Tuberculosis in Iran.


Proliferative kinetics of leukemia.

Proliferative kinetics of leukemia.


Stereotaxic surgery for generalised epilepsy.


Metabolic control mechanisms in mammalian systems. Regulation of pyruvate kinase in the rat cerebral cortex.


The nonhuman primate as an analogue for the study of periodontal disease.


General discussion. Usefulness and limitations of model systems.


Depolymerization of polysaccharides through the generation of free radicals at a platinum surface: a novel procedure for the controlled production of free-radical oxidations.


Ribonuclease I released from Escherichia coli by osmotic shock.


A polarization microscopic and micro x-ray diffraction study on the organic matrix of developing human enamel.


Studies of biological polyphosphate through the use of phosphorus-31 nuclear magnetic resonance.


Isolation of cell nuclei in coverslip cultures.


Primitive reflexes in parkinsonism.

Primitive reflexes in parkinsonism.


Ossification disturbances in children under antiepileptic treatment.


Transfer of antibody production with cells from bursa of Fabricius. F(1) hybrid chicks isogenic for the strong B histocompatibility locus and for most weak H-loci were X-irradiated on day 1 after hatching, injected intraperitoneally on day 2 with dispersed cells of bursa, spleen, or thymus from 4- or 10-wk-old F(1) hybrid donors, and immediately challenged by the same route with either Brucella abortus, sheep erythrocytes, or a mixture of both together. The agglutinin titers were measured in sera obtained 1 wk later. With 4-wk-old donors, a greater primary response to Brucella abortus was obtained after transfers of cells from bursa than from spleen, while thymus was much less effective. With 10-wk-old donors, the decreasing order of response was spleen, bursa, thymus. Only splenic cells were effective in transferring a response to sheep erythrocytes, at either donor age. In tests of synergism by cell mixtures from pairs of organs, the only positive finding was a modest augmentation of titer against sheep erythrocytes by bursa + spleen as compared with spleen alone. Bursal cells from 6- or 10-wk-old donors were effective in transferring a response to sheep erythrocytes when antigen injection was delayed until 5 days after cell transfer. Splenic cells from hormonally bursectomized donors were ineffective in transferring a primary response, even when the donors had been injected with antigen 1 wk before transfer. Preimmunization of normal donors led to marked increases in the responses to Brucella abortus produced by transferred splenic or thymic cells. With bursal cells, an increased response was obtained only if the interval between preimmunization and transfer was 17 rather than 7 days. With the 17-day interval, both bursal and thymic cells could also transfer a response to sheep erythrocytes. The primary sera to Brucella abortus produced after transfers of bursal or splenic cells contained almost entirely 19S antibodies. A 7S component was found in all the secondary sera tested.


Synergistic interaction of macrophages and lymphocytes in antigen-induced transformation of lymphocytes. The role of macrophages and lymphocytes in antigen-induced transformation of lymphocytes has been investigated. Lymphocytes and macrophages were obtained from inbred strain 13 guinea pigs which were either unimmunized or immunized with complete Freund's adjuvant (CFA) or tetanus toxoid in CFA. The transformation response to PPD or tetanus toxoid was assayed by tritiated thymidine incorporation. Addition of macrophages to immune lymphocytes significantly increased their response to purified protein derivative (PPD) or tetanus toxoid. This was observed if the macrophages were (a) "immune" or "nonimmune", (b) unirradiated or irradiated (3000 R), (c) 99% pure, and (d) peritoneal or alveolar in origin. Neither immune nor nonimmune macrophages were able to induce nonimmune lymphocytes to respond to PPD or tetanus toxoid. When macrophages were incubated with PPD or tetanus toxoid and then washed, they stimulated immune lymphocytes to transform. An incubation time of (1/2) hr was adequate, however, 2-4: hr was optimal. These studies indicate (a) that antigen-induced transformation of lymphocytes is greatly enhanced by macrophages; (b) that macrophage-antigen interaction can antecede lymphocyte-antigen interaction and results in macrophages which are able to stimulate lymphocyte transformation; and (c) that the immunological memory requisite to elicit specific transformation responses is a property of the lymphocyte and not the macrophage.


Host defense against bacterial endotoxemia: mechanism in normal animals. The present study defines the early response of normal rabbits to the intravenous injection of a single, sublethal dose of endotoxin. Within the first few hours following endotoxin there occurs in the circulating plasma of recipients a decrease in ionized calcium, a threefold increase in the heat-stable, organo-phosphate-resistant esterase level, and a striking increase in the endotoxin-detoxifying capacity. These results are fully consistent with the thesis that circulating plasma represents a principal site of detoxification and that plasma esterases of the nonspecific, carboxylic type are of major concern in defense against circulating endotoxins.


Long-term pacing in the management of bradyarrhythmias. Seven years' experience.


Adverse effects of xylitol in parenteral alimentation.


Suppression and stimulation of calcitonin secretion in medullary thyroid carcinoma.


A minimal medium for the growth of Plasmodium knowlesi in dilution cultures.


Diabetic ketoacidosis.

Diabetic ketoacidosis.


Characteristics of an Echovirus type 30 strain isolated during an epidemic of aseptic meningitis in New York in 1968.


Rifampin in initial treatment of pulmonary tuberculosis. A U.S. Public Health Service tuberculosis therapy trial.


Further exploratory studies in sarcoidosis. An epidemiologic investigation to compare the prevalence of tuberculosis infection and-or disease among contacts of matched sarcoidosis and asthmatic patients.


Acute aflatoxin B1 toxicity in the macaque and its similarities to Reye's syndrome.


Localization of methylated arginine in the A1 protein from myelin. Methylated arginine residues are found at only one site (position 107) of the polypeptide chain of the A1 protein, as shown by analysis of tryptic and peptic peptides; these analyses show 0.2 mole of N(G)-dimethylarginine and 0.4-0.8 mole of N(G)-monomethylarginine per mole of A1 protein. The methylated arginine residues appeared to be relatively resistant to tryptic attack. Both methylated derivatives were isolated from an enzymatic digest of the A1 protein; they were identified by chromatography, electrophoresis, and degradation to citrulline, methylamine, and ornithine on alkaline hydrolysis. The phylogenetic importance of the methylated derivatives was shown by their presence in the human, monkey, bovine, rabbit, guinea pig, rat, chicken, and turtle A1 proteins at the analogous position to that of the bovine sequence: [Formula: see text] We postulate that the methylated arginine residues may serve an important role in the myelin membrane in situ by stabilization of a double-chain structure for the A1 protein; such a double-chain conformation is induced by a (proline)(3) sequence located nearby.


LSD and genetic damage.

LSD and genetic damage. Of nine studies in vitro, six have indicated some degree of induced chromosomal breakage after exposure to LSD; three failed to confirm these results. The damage, when found, was generally of the chromatid type, arising during or after DNA synthesis. This damage, with one exception, was the result of concentrations of drug and durations of exposure which could not be achieved in humans with reasonable dosages. There did not appear to be a dose-response relation. The magnitude of damage, when found, was in the range encompassing the effects of many commonly used substances. The absence in vitro of excretory and detoxifying systems present in vivo, as well as several negative reports, cast doubt on the relevance of in vitro results. In 21 chromosomal studies in vivo, 310 subjects were examined. Of these, 126 were treated with pure LSD; the other 184 were exposed to illicit, "alleged" LSD. A maximum of only 18 of 126 (14.29 percent) of the subjects in the group exposed to pure LSD showed higher frequency of chromosome aberration than the controls. In contrast, a maximum of 90 of 184 (48.91 percent) of the subjects taking illicit LSD showed an increase in frequency of aberrations. Of all the subjects reported to have chromosome damage, only 18 of the 108 (16.67 percent) were exposed to pure LSD. The frequency of individuals with chromosomal damage reported among illicit drug users was more than triple that associated with the use of pharmacologically pure LSD. We conclude that chromosome damage, when found, was related to the effects of drug abuse in general and not, as initially reported, to LSD alone. We believe that pure LSD ingested in moderate dosages does not produce chromosome damage detectable by available methods. No significant work on carcinogenic potential of LSD has been reported so far. No cause-and-effect relation and no increase in the incidence of neoplasia among LSD users have been demonstrated. Case reports (three in 4.0 years) of leukemia and other neoplasia in this population are rare. The results of early chromosome studies suggested that true genetic damage might be a consequence of LSD exposure. The comprehensive evidence from studies on drosophila indicates no mutagenic effect from 0.28 to 500 microg of LSD per milliliter and a definite mutagenic effect from 2,000 to 10,000 microg/ml; this is consistent with a threshold response or a sigmoid dose-effect relation. We believe that LSD is, in fact, a weak mutagen, effective only in extremely high doses; it is unlikely to be mutagenic in any concentration used by human subjects. Circular dichroism experiments suggested that the specific mechanism of action of LSD on DNA may be a direct interaction resulting in conformational changes in the DNA helix. These changes are unlikely to result in a decrease of internal stability sufficient to cause breakage of chromosomes, but they may be the physical basis of the weak mutagenicity. Early chromosomal studies implicated LSD as a potential cause of congenital malformations, fetal wastage, and germinal chromosome damage. First reports of a teratogenic effect in hamsters and rats have not been confirmed. A review of 15 rodent studies indicated a wide range of individual, strain, and species susceptibility to the effects of LSD. The applicability of such investigations to man is doubtful. In a study of human pregnancies, those exposed to illicit LSD had an elevated rate of spontaneous abortions. There is no reported instance of a malformed child born to a woman who ingested pure LSD; there are six cases of malformation associated with exposure to illicit LSD, four of which have similar limb defects. Given, however, the high frequency of unexplained "spontaneous" birth defects, the rare occurrence of malformed infants born to women who used illicit LSD may be coincidental. While there is no evidence that pure LSD is teratogenic in man, the use of any drug during pregnancy requires that its potential benefits significantly outweigh its potential hazards. From our own work and from a review of the literature, we believe that pure LSD ingested in moderate doses does not damage chromosomes in vivo, does not cause detectable genetic damage, and is not a teratogen or a carcinogen in man. Within these bounds, therefore, we suggest that, other than during pregnancy, there is no present contraindication to the continued controlled experimental use of pure LSD. Note added in proof: A brief review has been brought to our attention. Although based on a sample of only 15 studies the author reached conclusions similar to our own (92).


Intravascular concentrations of calcium and magnesium ions and edema formation in isolated lungs.


A light and electron microscopic study of the nearly mature enamel of rat incisors.


Lactic dehydrogenase activity of aqueous humour in retinoblastoma.


Use of disodium cromoglycate in animal-sensitive asthmatics.


Anomalous urinary proteins in patients with serum M-components. Abnormal urinary proteins were investigated in 365 patients with serum M-components; 250 urines were available for study. All specimens were concentrated and processed by electrophoresis and immunoelectrophoresis. An overall frequency of Bence Jones proteinuria was found in 63%, whereas other M-components were detected in 18%. In patients with multiple myeloma, Bence Jones proteinuria occurred in 71%, while M-components in the form of whole molecules of immunoglobulins or as Fc and F'c fragments were observed in 20%. Bence Jones globulin was found in 60% of patients with IgG myeloma, 69% of those with IgA myeloma and 100% of patients with IgD myeloma and light chain disease. In regard to other diseases, Bence Jones proteinuria was found in 44% of patients with macroglobulinemia, in 60% of patients with nonplasmacytic neoplastic diseases and in 28% of patients with non-malignant conditions. Other M-components were detected in the urine of 13%, 14% and 6% respectively.The detection of abnormal urinary proteins in patients with M-components in the serum is an important diagnostic tool and may have prognostic significance.


Testicular degeneration in Macaca nemestrina induced by immobilization.


Some aspects of the endocrine control of the serum lipids, blood ketones and blood sugar in fed and fasted baboons, and of the mechanism of fuel control in fasting, with special reference to diabetes and lipaemia.


Antibodies to human prostatic acid phosphatase.


Nomarski interference contrast microscopy of human, monkey, and rabbit spermatozoa.


Effect of lesions in the temporal lobe and rhinencephalon on reproductive function in adult female rhesus monkeys.


Deafness due to Waldenstrom macroglobulinaemia.


Neurophysiological feature detectors and speech perception: a discussion of theoretical implications.


An economic survey of the nature and intent of the British National Health Service.


The current status of silicone injection therapy.


Tomorrow's health service.

Tomorrow's health service.


Hemolytic disease of the newborn due to anti-EW. A fourth example of the Rh antigen, EW.


Internal arteriovenous anastomoses for hemodialysis: technical modifications and results of two years' experience.


Spike-wave stupor. Petit mal status.

Spike-wave stupor. Petit mal status.


Pseudodiverticulum of the pharynx.

Pseudodiverticulum of the pharynx.


The separation of monkey prolactin from monkey growth hormone by affinity chromatography.


Data on structure of bovine LRH by inactivation.


Nuclear magnetic resonance studies of bovine carbonic anhydrase. Binding of sulfonamides to the zinc enzyme.


A 15-year report on fenestration of the oval window.


Transfer ribonucleic acid deficient in N6-(delta 2-isopentenyl)adenosine due to mevalonic acid limitation.


Partial purification, specificity and mechanism of action of the nisin-inactivating enzyme from Bacillus cereus.


Purification and properties of dihydroorotase from Escherichia coli B.


X-ray effects on ferredoxin from Clostridium Pasteurianum.


Treatment of gonorrhoea with two oral doses of antibiotics. Trials of cephalexin and of triple tetracycline (Deteclo).


Hutchinson's teeth and early treatment of congenital syphilis.


Coagulation and fibrinolytic systems in pre-eclampsia and eclampsia. The coagulation and fibrinolytic mechanisms were investigated in a group of patients with severe pre-eclampsia and eclampsia and the findings were compared with those of healthy women in late pregnancy. In patients with pre-eclampsia the following significant differences were found: (1) greater depression of plasma fibrinolytic activity (euglobulin lysis time) than in normal pregnancy, (2) a higher level of inhibitor to urokinaseinduced lysis, (3) increased levels of serum fibrin degradation products, and (4) reduced platelet counts.In patients with eclampsia a progressive increase of the level of serum fibrin degradation products was found over the three days following eclamptic seizures. No such increase occurred after grand mal seizures in late pregnancy. The findings in this study support the view that intravascular clotting is taking place in pre-eclampsia and that this disturbance of the balance between coagulation and fibrinolysis may be localized to certain areas of the vascular compartment, particularly the placental and renal circulations. Fibrin deposition in the maternal vessels supplying the placenta would impair the placental blood flow, which may explain the placental insufficiency which occurs in pre-eclampsia. Likewise fibrin deposition in the renal vasculature will result in glomerular damage and proteinuria. Hypertension may be related to the renal ischaemic changes or a compensatory response to the presence of fibrin deposition in the vascular compartment. This evidence of intravascular fibrin deposition raises the question of the possible therapeutic value of antithrombotic agents to inhibit the clotting process. On a theoretical basis such treatment might be expected to improve blood flow to the placenta and thereby fetal growth.


Diabetic ketoacidosis and influenza.

Diabetic ketoacidosis and influenza.


Effects of changes in feeding on the amino acid composition of protein in dental plaque from the monkey, Macaca irus.


A technique for tube-feeding newborn rats, and the effects of administration of various carbohydrate solutions on their subsequent caries susceptibility.


Genetically altered repression pattern of purine nucleotide synthesizing enzymes and inosine production in 8-azaguanine resistant mutants of Bacillus subtilis.


Kinetic studies by stopped-flow method of hydrolysis of 0-nitrophenyl alpha-maltoside catalyzed by saccharifying alpha-amylase from Bacillus subtilis.


A survey of ABO, MN, Q, Rh blood types in inhabitants of the Kaohsiung District of Taiwan.


Staff conference. St. Thomas Hospital. Parathyroid cysts within the thyroid gland. Total parenteral alimentation.


Maduromycetoma caused by Aspergillus nidulans.


The effect of anoxia on the ultrastructure of the superior cervical ganglion of the rat in vitro.


The effect of hyperoxia on the ultrastructure of the superior cervical ganglion of the rat in vitro.


Desmosomes, cilia and peculiar structure of membranes in erythroblasts of human fetal liver.


The fine structure of regenerating epithelium in the small intestine.


Banded structures in the connective tissue of lymphomas, lymphadenitis, and thymomas.


Effect of varying the cross match procedure upon detection of anti-D and anti-Kell.


A search for Lactobacillus acidophilus agglutinins in human saliva.


Relationship between renal structure and concentrating ability in the rhesus monkey.


Effect of prostaglandin E1 on renin in the dog.


Utility of the radioimmunoassay of serum thyrotrophin in man.


Evidence of a "silent" bacillemia in primary tuberculosis.


Reduced red cell glycolysis, 2, 3-diphosphoglycerate and adenosine triphosphate concentration, and increased hemoglobin-oxygen affinity caused by hypophosphatemia.


The biotransformation of halothane.

The biotransformation of halothane.


Pharmacologic studies with ABBOTT-30360, an analgesic-tranquilizer, and its analogues.


Anticonvulsant effect of phencarbamide and its modification by reserpine and tranylcypromine.


A method for production of antibody to hepatitis-associated antigen in rabbits.


Primary rhesus immunization of male volunteers.


Immunological homogeneity of membrane proteins from hereditary spherocytic, hereditary elliptocytic, and normal red cells.


Mediastinal emphysema during diabetic ketoacidosis.


Enhancement of algal growth in Cyanophyta-bacteria systems by carbonaceous compounds.


The effect of quaternary ammonium derivatives of bile acids on the rate of swelling of Pseudomonas aeruginosa in solutions of sodium and potassium salts.


Effect of dimethyl sulfoxide on the sclerotia of Sclerotium rolfsii.


Megavoltage technique for treatment of carcinoma of the post cricoid region.


The local prognosis after radical radiotherapy for squamous carcinoma of the alveolus and of the floor of the mouth.


Clinical and metabolic characteristics of hyperosmolar nonketotic coma.


Improvement in both insulin sensitivity and release following diabetic coma.


Natural antibodies to Escherichia coli in the pig.


Effect of carbenicillin, gentamicin, and their combination on experimental Pseudomonas aeruginosa urinary tract infection. The therapeutic efficacy of a combination of gentamicin with carbenicillin was compared with those of each antibiotic alone. The efferent urinary ducts of rats were infected with Pseudomonas aeruginosa and antibiotic therapy was started six days later. After seven days' therapy quantitative bacteriological findings in the urine and renal tissue were compared with those of untreated rats that had been infected. The degree of infection was least in the group treated with concurrent gentamicin and carbenicillin. It was concluded that the therapeutic efficacy of the gentamicin-carbenicillin combination exceeded those of each antibiotic administered alone.


The preservation of gonococci in liquid nitrogen. Gonococci suspended in 1% proteose peptone containing 8% glycerol can be snap frozen in liquid nitrogen without detectable loss on thawing. This recovery rate has allowed the use of frozen organisms as the starting inoculum for the bulk growth of gonococci and to preserve gonococci both in urethral pus and after primary subculture for use in studies on gonococcal virulence. The method described for freezing urethral pus on charcoal swabs should make it possible to transport infected specimens from areas lacking adequate laboratory facilities.


Fate of transforming DNA following uptake by competent Bacillus subtilis. I. Formation and properties of the donor-recipient complex.


Bony metaplasia in trachea and bronchi.

Bony metaplasia in trachea and bronchi.


Studies on the stabilizing forces of simple RNA viruses. II. Stability, dissociation and reassembly of cucumber mosaic virus.


Antigenicity, toxicity and cross-reactions of male bat (Myotis lucifugus) reproductive organs.


Oxidation of pyruvate and glucose by oocytes of the mouse and rhesus monkey.


Renal carcinoma in an owl monkey (Aotus trivirgatus).


Prostaglandins in fertility.

Prostaglandins in fertility.


Introduction to the actions of kinins and prostaglandins.


Incontinentia pigmenti (Block-Sulzberger syndrome) with electron microscope findings.


The anaesthetist's effect upon the cerebral arteries.


An epidemic of hepatitis in a renal dialysis unit.


Aggressive therapy for Hodgkin's disease.


Antigenic properties of Bacillus licheniformis cell wall components.


The elastase-like enzymes from Streptomyces griseus (pronase). Isolation and partial characterization.


Studies on hog intestine mucosa peroxidase.


Trimethoxybenzene derivatives from indigenous drugs.


Ampicillin in enteric fever.

Ampicillin in enteric fever.


Phenoloxidase activity and fruiting body formation Schizophyllum commune. Studies of induced haploid fruiting in the Basidiomycete Schizophyllum commune suggested that polyphenoloxidase enzymes may participate in the formation of fruiting bodies. Reported inhibitors of phenoloxidases were found to affect both the activity of these enzymes and the fruiting response. Fertile mycelia were found to give a positive Bavendamm reaction in situ, and cell-free extracts from fertile mycelia exhibited polyphenoloxidase activity when studied spectrophotometrically. Sterile mycelia, on the other hand, were devoid of these activities. Various kinds of mutant homo- and dikaryotic mycelia were studied, and the results suggested a correlation between phenoloxidase activity and fruiting body formation in S. commune.


Colicin tolerance induced by ampicillin or mutation to ampicillin resistance in a strain of Escherichia coli K-12. A mutant (G11el) of Escherichia coli selected as being resistant to ampicillin and showing signs of an envelope defect was also found to be tolerant to colicins E2 and E3. The colicin tolerance of G11el could be partially repressed by Mg(2+) ions. Transition from tolerance to sensitivity and vice versa by shifting the concentration of Mg(2+) in the growth medium required several generations. This indicated that synthesis of new envelope material was needed for transition. Previous physiological results have indicated a change in the envelope lipopolysaccharide (LPS) of G11el. However, chemical analyses revealed no differences in carbohydrate composition between LPS from G11el and its parent strain G11al. Genetic experiments showed that the mutation in G11el is located at about 20 min on the E. coli K-12 chromosome. The mutation was dominant over wild type in partial diploids with the mutation located on the episome. Because colicin tolerance was the most striking phenotypic effect as a result of mutation in the actual locus, this gene will be named tolD until the exact gene product is known. Spheroplasts formed from G11al and G11el by ethylenediaminetetraacetate-lysozyme treatment did not adsorb colicin E2; however, penicillin spheroplasts of G11al and G11el were tolerant to colicin E2. Thus, colicin tolerance can be induced biochemically. It is suggested that colicin tolerance often is a secondary consequence of a change in the cell envelope.


Induction of mutants of Staphylococcus aureus 100 with increased ability to produce enterotoxin A. As a result of serial exposures to a mutagenic agent, N-methyl-N'-nitro-N-nitrosoguanidine, the yield of enterotoxin A produced by the last mutant in the series was increased nearly 20-fold over the amount produced by the parent Staphylococcus aureus 100.


Pseudogermination in dipicolinic acid-less spores of a Bacillus cereus T mutant. Dipicolinic acid-less spores of a Bacillus cereus T mutant in 33 mm sodium bicarbonate (pH 8.5) underwent pseudogermination (germination-like changes) accompanied by ribonucleic acid leakage and loss of viability.


Effect of cord factor (mycobacterial toxic glycolipid) on liver microsomes in vivo.


Velocity of chromosome replication in thymine-requiring and independent strains of Bacillus subtilis. Chromosomes in spores of a thymineless mutant of Bacillus subtilis strain 168 were shown to have a replication fork, unlike chromosomes in spores of the thy(+) strain which are in a complete form. As a consequence the number of replication forks in germinating cultures is higher in the thy(-) strain than in the thy(+) one. Chromosome replication time (C) in the thy(+) strain was found to be about 53 min for growth rates from 20 to 60 min. In the thy(-) strain, C was about 75 min at high growth rates and increased with decreasing growth rate when the thymine concentration was not limiting. With limiting thymine concentrations in the medium replication velocity decreased independently of growth rate.


Endogenous dark respiration of the blue-green alga, Plectonema boryanum. Endogenous dark respiration in the blue-green alga Plectonema boryanum is markedly affected by preincubation in the light: it can be increased from a basal rate of 5 nmoles of O(2) to 55 nmoles of O(2) per mg of cell protein per min after exposure of the cells to light for 8 to 10 hr. Under conditions of enhanced dark respiration, cyanophage multiplication in the dark increases drastically and approaches the cyanophage yields obtained in photosynthesizing Plectonema cells. This implies that the biosynthetic capabilities of the algal cells, at least with respect to viral synthesis, can be similar in the dark to those in the light. The enhanced endogenous respiration rate was found to be dependent on photoassimilation of CO(2) and on protein synthesis. The implications of these findings with respect to obligate photoautotrophic metabolism in blue-green algae are discussed.


Survey and follow-up of 225 consecutive patients with a depressed skull fracture.


"Cryptic tubers" as a cause of focal epilepsy.


Collection of oviduct fluid from unrestricted monkeys.


An annual rhythm in the sexual activity of the male rhesus monkey, Macaca mulatta, in the laboratory.


Epilepsy after missile wounds of the head. Two hundred and thirty-seven Nigerians with missile head injury were treated at the University College Hospital (UCH) Ibadan, between July 1964 and January 1970. Among the 165 of these patients who have been followed up for over a year, the incidence of epilepsy has been 11·4%, 18·75%, and 33·3% in a follow-up period ranging between one to two years, two to three years, and three to five years respectively.Wounds in the parietal region, especially those at the vertex, were followed by epilepsy more frequently than injuries elsewhere on the head. Of these wounds, tangential skull injuries and through-and-through brain injuries were more epileptogenic than others.Fits which occurred within a fortnight of wounding have been designated as `early' fits, and they appeared to differ in their aetiology from `late' fits.


Megestrol acetate: its effect on the inhibition of ovulation in squirrel monkeys, Saimiri sciureus.


Rh-incompatible platelet transfusions--risks and consequences of sensitizing immunosuppressed patients.


Animal models for atherosclerosis. A review.


Urinary estrogens. II. Excretion of estrogens in rhesus monkeys stimulated with human gonadotropins.


New selective medium for isolation of L. monocytogenes.


Investigations on the survival times of Listeria bacteria in suspensions of brain tissue, silage and faeces and in milk.


Experimental approach in studies on pathogenesis of bacillary dysentery--with special references to the invasion of bacilli into intestinal mucosa.


Individuality in some hypophaptoglobinaemia-sera of Sierra Leone African population.


Immunologic properties of plasminogen.

Immunologic properties of plasminogen.


Cerebral ischemia and reactive hyperemia. Studies of cortical blood flow and microcirculation before, during, and after temporary occlusion of middle cerebral artery of squirrel monkeys.


Red cell organic phosphate levels and in vivo oxygen-hemoglobin dissociation in patients on maintenance dialysis with anaemia.


Multiple epiphyseal dysplasia in a litter of Beagle puppies.


The effects of culture media on Saprolegnia antigens.


Diagnostic value of muramidase.

Diagnostic value of muramidase.


Plumbism exists today.

Plumbism exists today.


Responses of monkey thalamus to nociceptive stimuli.


An examination of the influence of husbandry on the plasma immunoglobulin level of the newborn calf, using a rapid refractometer test for assessing immunoglobulin content.


The size and morphology of T.G.E. and vomiting and wasting disease viruses of pigs.


Brucellosis: a possible symptomless carrier.


Pathologic sequelae of acute cardiac irradiation in monkeys.


The nerve sheath tumor, solitary and in von Recklinghausen's disease; a unitary mesenchymal concept.


Preparation of hyperalimentation solutions for the pediatric patient.


Rational intravenous therapy.

Rational intravenous therapy.


The pharmacist and a nutritional intravenous therapy program.


Reduction in serum CO2 content during hyperalimentation therapy.


Long term follow-up of patients with menorrhagia treated by irradiation.


Unusual bone trabeculation in a patient with macroglobulinaemia simulating fibrogenesis imperfecta ossium.


Experimental analysis of the extinction phenomenon in monkeys.


Protein synthesis in isolated rat brain mitochondria and nerve endings.


Tactile direction sensitivity of area 2 oral neurons in the rhesus monkey cortex.


Rhythmic unit firing in the precentral cortex in relation with postural tremor in a deafferented limb.


An endoscopic evaluation of gastric ulcer treated with a mixture containing bismuth ammonium citrate.


The use of isosorbide dinitrate in status anginosus.


Metiapine: a new antipsychotic agent.

Metiapine: a new antipsychotic agent.


Piperacetazine (Quide) in the management of behavioral disorders in mildly retarded institutionalized boys.


Combined administration of thioridazine and fluoxymesterone in the treatment of geriatric patients.


A controlled study of fluspirilene in chronic schizophrenia.


Immunizing capacity of B. abortus S99 sodium chloride extract against B. abortus infection in guinea-pigs.


Immuno-electrophoretic characterization of Brucella species: chemical extract.


Electrophoretic characterization of Brucella sonic extract.


Observations of the vibration of the basilar membrane in squirrel monkeys using the Mössbauer technique.


Temporal position of discharges in single auditory nerve fibers within the cycle of a sine-wave stimulus: frequency and intensity effects.


Nonspecific inhibition of enzymes by organic contrast media.


Benzothiazolines as antituberculous agents.


Synthetic biologically active polymers. 7. Antibacterial activity of some sulfonamide-formaldehyde copolymers.


Charge-spatial models. 2. cis-and trans-3-and-4-carboxycyclohexyl phosphates and the carboxylate esters as analogs of 2'-deoxyuridine 5'-phosphate.


Studies on the biology of the periodontium of marmosets. X. Cortisone induced periodontal and skeletal changes in adult cotton top marmosets.


Antigenic substance in urine of rabbits infected with Schistosoma japonicum.


Collie eye anomaly.

Collie eye anomaly.


The role of the tryptophan residues in the activity of the nerve growth factor.


The properties and modification of tryptophan in a sea snake toxin, erabutoxin a.


The effect of divalent ions on the isolation of proteins from rat liver nucleoprotein.


Regional blood flows of the rhesus monkey during halothane anesthesia.


Etoxadrol (CL-1848C) a new dissociative anesthetic: studies in primates and other species.


[Effects of lesions of the median hypothalamus on the conditioned emotional reaction in the white rat] (Fre].


The loss of tuberculin sensitivity in certain patients with active pulmonary tuberculosis.


Ketosis causing spurious elevation of SGOT values.


Central venous catheters in children--their placement and care.


Enzymic purification of a hybrid between ribosomal ribonucleic acid and deoxyribonucleic acid.


Characterization of the messenger ribonucleoprotein released from reticulocyte polyribosomes by EDTA treatment.


Health rules for monkeys and apes.

Health rules for monkeys and apes.


"Intal" in allergic rhinitis.

"Intal" in allergic rhinitis.


Vitamin B12 uptake by intestinal microorganisms: mechanism and relevance to syndromes of intestinal bacterial overgrowth. The mechanism of bacterial uptake of vitamin B(12), the spectrum of microorganisms capable of such uptake, and the factors involved were the subject of this study. Bacterial uptake of vitamin B(12) was found to be at least a two stage process. A primary uptake phase which was rapid (1 min or less), pH dependent, nontemperature dependent, did not require viable organisms and was insensitive to either the metabolic inhibitor dinitrophenol or to the sulfhydryl inhibitor N-ethyl-maleimide. Protein denaturation (formalin treatment or autoclaving) abolished all B(12) uptake. This primary uptake phase is thought to represent adsorption to binding or "receptor" sites on the cell wall. Second stage uptake was slower, pH and temperature dependent, required living bacteria, and was abolished by either dinitrophenol or N-ethyl-maleimide. This phase is dependent upon metabolic processes and may reflect transfer of B(12) from surface "receptor" sites into the bacterial cell. Although differences among organisms were observed in total 1 hr uptake, number of surface "receptor" sites, and relative avidities for B(12), all organisms except Streptococcus fecalis shared the two stage mechanism. Two Gram-positive organisms. Bacillus subtilis and Group A streptococcus, demonstrated the highest 1 hr vitamin B(12) uptake values; Gram-negative bacteria required 2,000-10,000 the number of organisms for comparable uptake. Binding constants (K(m)) varied from 5.05 +/-1.67 x 10(-10)M for B. subtilis to 6.18 +/-3.08 x 10(-9)M for Klebsiella pneumoniae which approximate the Km for human intrinsic factor (0.38 x 10(-10)M). Competition between bacteria and intrinsic factor for vitamin B(12) may be inferred from the similarity of these constants. These observations suggest that a variety of enteric and nonenteric organisms, not requiring exogenous B(12), may play a role in the pathogenesis of the vitamin B(12) malabsorption found in the intestinal bacterial overgrowth syndromes.


Concepts of a state "foundation plan". A method of health care delivery.


Radioimmunoassay of human chorionic somatomammatropin (HCS) plasma levels in normal and abnormal pregnancies.


The oldest fossils.

The oldest fossils.


Rationale for treatment of ulcerative keratitis. II.


Febrile agglutinins in narcotic addicts.


Serum copper and ceruloplasmin in pregnancy.


Effect of ethanol upon uterine activity and fetal acid-base state of the rhesus monkey.


Placental transfer and neonatal elimination of diphenylhydantoin.


Influence of copulation on time of ovulation in women.


Search for the L-phase of Streptococcus faecalis in kidneys of rats experimentally infected with the bacterial phase and treated with penicillin.


Interactions between pesticides and wildlife.


The genetics of animal viruses.

The genetics of animal viruses.


Metabolism studies on 3-chloromethyl heptynol-3-carbamate (BRL. HN 1212).


Duodenal diverticulum treated by excision of mucosal pouch only.


Plasma and pancreatic insulin concentrations in adult squirrel and rhesus monkeys.


Immunological adjuvants. IV. Relationship between adjuvant activity and antigenicity in mycobacterial adjuvant.


Chemotherapy of experimental Toxoplasma gondii (RH strain) infection.


Etiological factors in osteoradionecrosis.


The vent blade implant.

The vent blade implant.


Oral medicine seminar No. 24. Efficacy of topical antibiotics in dentistry.


52 years of intermittent pain and swelling and enlargement of the jaws--diagnosis and a cure.


A review of continuing education programs in Connecticut.


Macrophage inhibition produced by guinea pig after sensitization with a larval antigen of Trichinella spiralis.


Necator americanus infection in primates.


Observations on the relapse activity of Plasmodium fieldi in the rhesus monkey.


Perspectives and important needs in parasite chemotherapy.


Current research review. Lung transplantation.


Viral properties of scrapie.

Viral properties of scrapie.


Cellular effects of a protozoan-produced growth-stimulating factor.


A method for the detection of syphilis in the dental office.


Biological properties of antibody classes in transplantation immune sera.


Hemopoietic chimerism in imported and laboratory-bred marmosets.


Immunological tolerance and rejection of skin allografts in hhe marmoset.


Heterologous antilymphocyte serum. Influence of immunogenicity on immunosuppressive properties.


The fine structure of the thymus of the fetal and neonatal monkey (Macaca mulatta).


Heparin-induced increase of diamine oxidase in lymph and blood plasma of rat, guinea-pig and rabbit.


Evidence for seasonal variation in aggressive behaviour by Macaca mulatta.


Virus-like particles associated with a faecal antigen from hepatitis patients and with Australia antigen.


The binding of Ca2+ to the cell membrane increased by volatile anesthetics (alcohols, acetone, ether) which induce sensitization of nerve or muscle.


Properties of inosine-5'-phosphate dehydrogenase from Bacillus subtilis.


Defibrillatory treatment of exertional cardiac arrest in coronary disease.


Tetracyclines, L-forms, and bacterial infections.


Dihydrogenated ergot alkaloids (Hydergine) and cerebrovascular insufficiency.


Effect of small-dose hyperosmotic injections on intraocular pressure of small animals and man when optic nerves are transected and intact.


Heterocyst formation and nitrogenase synthesis in Anabaena sp. A kinetic study.


Growth responses of blue-green algae to sodium chloride concentration.


Reaginic antibodies in dogs infected with Echinococcus granulosus.


The effect of iron and transferrin on the killing of Escherichia coli in fresh serum.


Antibody production by lymph-borne immunoblasts following subcutaneous injection into xenogeneic recipients.


A method for the isolation of plasma membrane of animal cells.


Comparative studies on the Tyzzer's organisms from rats and mice.


Immunological adjuvants. V. A critical evaluation of minimal requirements for mycobacterial adjuvants to induce delayed hypersensitivity.


EDTA and penicillin resistance of Staphylococcus aureus--a reevaluation.


The relationship of reflux esophagitis to pharyngeal pouch (Zenker's diverticulum) formation.


Failure of Australia antibody to prevent infectious hepatitis.


Empiric therapy with carbenicillin and gentamicin for febrile patients with cancer and granulocytopenia.


Use of various concentrations of culture filtrates of tubercle bacilli for detection of precipitins in the blood serum of tuberculous patients.


Experimental cancer chemotherapy: strategy for cure.


Surface ultramicroscopy of paroxysmal nocturnal haemoglobinuria erythrocytes.


Thalassaemia and high F-gene in Aleppo.

Thalassaemia and high F-gene in Aleppo.


Red cell metabolism in anaemia. The oxygen uptake in the presence of methylene blue.


Multiple myeloma in one of a pair of monozygotic twins.


Isolations of Silverwater virus from naturally infected snowshoe hares and Haemaphysalis ticks from Alberta and Wisconsin.


Silverwater virus serology in snowshoe hares and other vertebrates.


Cephaloglycin and its biologically active metabolite desacetylcephaloglycin. Chromatographic studies and microbiological assays show that, after oral administration, cephaloglycin is partially converted in man to a biologically active metabolite desacetylcephaloglycin. The antibacterial activity of this metabolite compared to that of cephaloglycin is equivalent against gram-positive organisms but is lower against gram-negative bacilli. Successful therapy of urinary tract infections with cephaloglycin must be mainly attributed to the antibacterial activity of this metabolite. At the present time, it is not possible to assess what influence low amounts of unaltered cephaloglycin have on the outcome of therapy.


Therapy of staphylococcal infections in monkeys. VI. Comparison of clindamycin, erythromycin, and methicillin. Intravenous inoculation of a penicillin-resistant, phage type 80/81 staphylococcus caused lethal infection in six of eight untreated monkeys. Daily intragastric administration of clindamycin hydrochloride and erythromycin stearate and intramuscular inoculation of clindamycin-2-phosphate and methicillin, all at a dose level of 50 mg/kg, was followed by mortalities of one of eight, one of eight, none of eight, and one of eight monkeys, respectively. Duration of obvious acute illness in surviving monkeys and time required for complete recovery were not significantly different in the four therapy groups with the exception that duration of acute illness in monkeys treated with clindamycin-2-phosphate (mean, 4.1 days) was significantly shorter than in monkeys given erythromycin stearate (mean, 7.1 days). In vitro sensitivity data and serum antibacterial levels would suggest that methicillin would be the least effective therapeutically, followed by erythromycin stearate and the two clindamycin preparations in that order. However, this prediction was not fulfilled in these studies in experimentally infected monkeys.


Aerosol survival of Pasteurella tularensis disseminated from the wet and dry states. The aerosol survival in air and in nitrogen was measured for Pasteurella tularensis live vaccine strain, disseminated from the wet and dry states. The results showed that most of the loss of viability occurred in less than 2 min of aerosol age, i.e., a rapid initial decay followed by a much slower secondary decay. In nitrogen and air, minimum survival occurred at 50 to 55% relative humidity (RH) for wet dissemination and at 75% RH for dry dissemination. This shift indicated that aerosols produced by wet and dry dissemination were not equivalent and suggested that survival might not be related to bacterial water activity or content. The results showed that rehydration is the key process with regard to survival, but that lysis on rehydration is not a primary death mechanism. The effects of oxygen were complex because it could be either protective or toxic, depending upon other conditions. The protective action of oxygen was through an effect on the spent culture suspending fluid. The latter contained a toxic component, the activity of which is suppressed by oxygen; possibly the component is pumped away during freeze-drying. A toxic effect of oxygen was not found in the presence of spent culture media because the toxicity of the latter masks such an effect. With other bacterial suspending fluids, oxygen was shown to be toxic at low RH. Similar effects with regard to oxygen toxicity were also found with a laboratory strain of P. tularensis. Differences in oxygen toxicity for aerosols generated from the wet and dry states also suggest that bacterial water content and activity do not control aerosol survival.


Listeria monocytogenes in nature.

Listeria monocytogenes in nature. Samples from 12 farms were examined during two successive spring and early autumn seasons. L. monocytogenes was isolated from vegetation or soil taken from 11 of the 12 farms and from 6 of the 7 nonagricultural sites. A total of 27 strains were isolated from the 19 sites. The organism was not isolated from any of the autumn collections.


In vitro susceptibility of Corynebacterium diphtheriae to thirteen antibiotics. Fourteen isolates of Corynebacterium diphtheriae proved to be most susceptible to erythromycin and rifampin and were also inhibited by 11 other antibiotics tested.


Removal of Nonidet P40 absorbance for optical density monitoring of cytoplasmic ribonucleic acid. Absorbance at 260 nm due to Nonidet P40 in cytoplasmic extracts can effectively be reduced by extraction with CHCl(3) without appreciable loss of ribonucleic acid.


Capillary tube assay for staphylococcal enterotoxins A, B, and C. A miniaturized single-gel diffusion procedure for detection of staphylococcal enterotoxin is proposed. The technique effects substantial savings of reagents and is easy to perform.


Effects of parenteral hyperalimentation on upper gastrointestinal tract secretions.


Utilization of osmometry in critically ill surgical patients.


Acute pulmonary insufficiency. Treatment in Vietnam casualties.


Antigens of Vibrio parahaemolyticus. II. Existence of two different subunits in the flagella of Vibrio parahaemolyticus and their characterization.


Infection of simian adenovirus SA7 in hamster kidney and embryo cells.


Studies on peptides, glycopeptides and antigenic polysaccharide-glycopeptide complexes isolated from an L-11 enzyme lysate of the cell walls of Mycobacterium tuberculosis strain H37Rv.


Demonstration of viral antigen in giant cells formed in monkeys experimentally infected with measles virus.


Rabbit antiserum against Australia antigen.


Modification of staphylococcal nuclease with nitrophenylsulfenyl halides.


Nitroglycerin (trinitrine): cardiovascular effects and plasma levels in the rabbit.


Demonstration of two types of DNA repair in X-irradiated Micrococcus radiodurans.


Electron microscopy of osteocytes and the pericellular matrix in rat trabecular bone.


Lysis of Bacillus subtilis, Escherichia coli, and Staphylococcus aureus by phenethyl alcohol.


Effects of glutamic acid on sporulation of Bacillus cereus and on spore properties.


Giant and duplicated imaginal discs in a new lethal mutant of Drosophila melanogaster.


Free behavior of rhesus monkeys following lesions of the dorsolateral and orbital prefrontal cortex in infancy.


The carbon dioxide hydration activity of carbonic anhydrase. I. Stop-flow kinetic studies on the native human isoenzymes B and C.


Activators of yeast hexokinase.

Activators of yeast hexokinase.


Studies on the induction and repression of enzymes in rat liver. VI. Some properties and the metabolic regulation of two isozymic forms of serine dehydratase.


The dinitrophenylation of human carbonic anhydrase B.


Effect of different immunization procedures on agglutination and precipitation reactions of Trichomonas gallinae.


Determining effect of growth medium on the shape and position of daughter chromosomes and on sporulation in Bacillus subtilis.


Brachiation and the early evolution of the hominoidea.


Transmission of two subacute spongiform encephalopathies of man (Kuru and Creutzfeldt-Jakob disease) to new world monkeys.


Distribution of marihuana in monkey brain and concomitant behavioural effects.


Acetyl-CoA carboxylase activity in the liver of meal-eating and nibbling rats.


X-ray-induced teratogenesis in the mouse and its possible significance to man.


A clinical trial of disodium cromoglycate (Lomudal) in asthmatic children.


Unwinding of DNA by actinomycin D binding.


Renaturation of DNA from the blue-green alga Lyngbya sp.


Enzymic modification of chromosomal macromolecules. II. The formation of histone epsilon-N-trimethyl-L-lysine by a soluble chromatin methylase.


The immunosuppressive effects of long-term combination chemotherapy in children with acute leukemia in remission.


Minimum inhibitory concentrations of carbenicillin against Pseudomonas aeruginosa. Investigations on the inoculum effect.


Studies on the glutamylpeptide antigen (GPP) in the genus Bacillus. The frequency of its occurrence and presence in the cell wall.


The production of protease by dense suspensions of Bacillus megaterium KM Sp.


Immunochemical studies on the double specificity of allotype a2 of rabbit immunoglobulin G.


Detection of antigenic subunits of a murine leukemia virus by passive hemagglutination-inhibition.


Immunochemical comparison of human erythrocyte membrane proteins.


Influenza virus encephalitis in squirrel monkeys receiving immunosuppressive therapy.


Hormone-influenced serum protein (HIP): anti-protein system in the chicken.


Oxidative sulfitolysis of human IgM.

Oxidative sulfitolysis of human IgM.


Studies of immune mechanisms in leprosy. 3. The role of cellular and humoral factors in impairment of the in vitro immune response.


Immune hemolysis and the functional properties of the second (C2) and fourth (C4) components of complement. 3. The hemolytic efficiency of human and guinea pig C2 and C4.


Quantitative determination of platelet fibrinogen.


Measurement of elastase activity by elastin agar and its use in the detection of antitrypsin deficiency.


A study on the lymphatic apparatus in the pancreas of Macaca cyclopis, with special reference to the development.


Cholesterol metabolism in the baboon.

Cholesterol metabolism in the baboon.


Changes in serum immunoglobulin after myocardial infarction.


Hemoglobin A2-Indonesia or alpha 2 delta 2 69(E13) Gly--Arg.


The effect of thermal denaturation on the antigenicity of glycinin.


Partial characterization and cellular localization of two deoxyribonucleases in the small intestine of the rat.


A comparative trial of furazolidone and chloramphenicol in typhoid and paratyphoid fever.


Tissue dissociation with proteolytic enzymes. Adsorption and activity of enzymes at the cell surface.


RNA of Physarum. I. Preparation and properties.


Nonspecificity of cation depletion when using chelators in biological systems.


Neurofibromatosis (von Recklinghausen's disease) and bleeding from the gastrointestinal tract.


Congenital diverticulum of the choledochus. Report of a case.


Some species differences in fibrinolysis and blood coagulation.


An evaluation of inert and resorbable ceramics for future clinical orthopedic applications.


Further studies on the ultrastructure of dimeric IgA of human origin. Human colostral IgA and myeloma dimer IgA were purified and examined in the electron microscope using a modified technique of negative staining. Both types of preparation contained double Y-shaped structures of the dimensions: Fab region, 35 x 70 A, and the sum of the two Fc regions, 40 x 140-155 A. Colostral IgA as well as myeloma dimer IgA molecules showed a tendency of bending at the point where the Fc regions joined. Secretory component bound to dimer IgA produced no visible alteration of the molecule. Mild reduction and alkylation of colostral IgA yielded single Y-shaped 7S monomers with the dimensions, Fab, 35 x 70 A, and Fc, 40 x 65-70 A.


Synthesis and assembly of immunoglobulins by malignant human plasmacytes and lymphocytes. II. Heterogeneity of assembly in cells producing IgM proteins. Bone marrow or lymph node cells from 10 patients whose sera contained large amounts of monoclonal IgM proteins were incubated with radioactive amino acids in short-term tissue culture. Samples of soluble cytoplasmic extracts and secreted material were examined by immunologic precipitation with specific antisera, acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, and sucrose gradient centrifugation. In all samples studied, 8S IgM was the major intracellular precursor of the fully assembled 19S protein. Cells obtained from some patients contained little or no fully assembled 19S protein intracellularly; however, cells from most patients seemed to accumulate fully assembled 19S molecules intracellularly before secretion. Secreted material from both groups contained large amounts of 19S IgM. The differentiation between accumulating and nonaccumulating cells did not correlate with heavy or light chain antigenic type. Synthesis and assembly appeared to be identical in cells obtained from different anatomic sites in the same patient Studies carried out in one patient before and after therapy revealed no qualitative differences in the pathway of assembly and reflected only a decrease in the total number of immunoglobulin-producing cells.


Epidemiological study of anonymous mycobacteria infection using three types of tuberculins.


Comparative serological studies of some strains of Tahyna virus. I.


Study of Pseudomonas aeruginosa-haemolysin by electrophoresis.


The AU-SH antigen in patient sera from a prospective study on viral hepatitis.


The balance sheet in employee health conservation.


Chemistry of cephalosporin antibiotics. XXI. Conversion of penicillins to cephalexin.


The prevention of recurrent pterygia.

The prevention of recurrent pterygia.


Heart rate derived from blood pressure and other physiological signals.


Recent developments in serologic methods for the diagnosis of coccidioidomycosis. 3. A soluble antigen fluorescent antibody test.


Clinical laboratory studies of the gorilla, chimpanzee, and orangutan.


Effects of alcohol and nitroglycerin on vascular responses in man.


Repeated stimulation of amygdala in awake monkeys.


Afferent inhibition in the cuneate nucleus of the rhesus monkey.


Tremor at rest following cerebellar lesions in monkeys: effect of L-DOPA administration.


Further studies on the abnormal factor X (factor X Friuli) coagulation disorder: a report of another family.


A comparison of brain and liver catechol O-methyltransferase.


Characterization of the esterases of canine serum.


Questionnaire on cancer.

Questionnaire on cancer.


Fungal metabolism of flavonoids. Purification, properties, and substrate specificity of an inducible laccase from Polyporus versicolor PRL 572.


The case against delayed operation for breast cancer.


Method of evaluating children with Hodgkin's disease.


Unproven methods of cancer management. Kanfer neuromuscular or handwriting test.


Studies on the meningococcal polysaccharides. I. Composition and chemical properties of the group A polysaccharide.


Regulation of aspartokinase in Bacillus subtilis. The separation and properties of two isofunctional enzymes.


Definition of cow's milk protein fraction evoking precipitating antibodies.


Human antibodies to ruminant IgM concealing the absence of IgA in man.


Comparative evaluation of commercial precipitating antisera against human IgA.


A radioimmunoassay for C1 esterase.

A radioimmunoassay for C1 esterase.


The management of prostatic nodules.

The management of prostatic nodules.


The effects of chewable isosorbide dinitrate in coronary insufficiency. A report on exercise electrocardiograms in 13 patients.


Cerebral paragonimiasis.

Cerebral paragonimiasis.


Major histocompatibility locus of rhesus monkeys (RhL-A).


Conversion of tyrosine to L-dihydroxyphenylalanine using immobilized tyrosinase.


Cervical nodal metastases of unknown origin.


Failure to transfer bullous pemphigoid with serum from patients.


Inhibition of the elastase-like esterase in human leukocyte granules by human leukocyte cell sap.


Changes in the somatosensory cortical evoked potential produced by hypovolemic shock.


Phenylalanine tolerance tests in simian primates.


IgM antibodies in fish mucus.

IgM antibodies in fish mucus.


Herpesvirus saimiri. 3. Plaque formation under multi agar, methyl cellulose and starch overlays.


The effects of DL-1-(4-hydroxyphenyl)-2-isopropylaminoethanol HCl (AL842) on intraocular pressure and pupil size of rhesus monkeys.


Structuring for.....continuing education within the hospital nursing service.


Structuring for.....an interdisciplinary on-ward approach to inservice education.


Structuring for.....continuing education within the professional association.


Structuring for.....continuing education programs within the american heart association.


Some thoughts on structuring for continuing education in nursing.


Structuring for....continuing education in nursing within the university.


A nursing inservice education program: policy of operation.


Continuing education: necessity and opoortunity.


Confrontation and search: a teaching-learning methodology.


Interprofessional continuing education.

Interprofessional continuing education.


The lengthened line: thrust-a must.

The lengthened line: thrust-a must.


Editorial: interprofessional education.

Editorial: interprofessional education.