Vascular responses to acute intracranial hypertension. In 27 rhesus monkeys the cerebrospinal fluid pressure (CSFP) was raised by injections into the cisterna magna to about 40 to 50 mm Hg in steps of 5 mm Hg every five minutes. During the initial phase of the rise of the CSFP to about 15 mm Hg normal animals showed a significant fall in the systolic arterial blood pressure. With a further elevation of the CSFP the BP rose till the CSFP reached 30 to 40 mm Hg. If the CSFP were raised higher than that, a large number of the animals showed a significant fall in the BP. In animals which were shocked before the CSFP was raised there was no drop in the systolic BP during the initial phase. This study indicates that vascular decompensation occurs in the majority of animals when the CSFP goes higher than 30 to 40 mm Hg; there is a significant rise in the pulse rate, superior sagittal sinus pressure (SSP), and internal jugular vein pressure (JVP). The JVP was related to the SSP, indicating that the JVP most probably reflected the pressure changes in the intracranial venous sinuses. Four animals suddenly collapsed at the highest CSFP. In the remaining 23 animals, on a sudden lowering of the CSFP to zero from the highest level, 13 monkeys died in less than half an hour and four in about an hour, while six animals stood this elevation of the CSFP well, with a good recovery. This indicates that, once the vascular decompensation has set in, the prognosis is generally poor even after lowering the CSFP to normal. The drop of the CSFP to zero produced no significant change in the pulse rate but a significant fall in the BP. The SSP rose when its pre-lowering level was less than 7·5 mm Hg and fell when the level was at or above 7·5 mm Hg level. The JVP showed a significant correlation with the variations in the SSP. The fundus examination at the end of the experiment revealed no abnormality.


Activation of the electroencephalogram with intravenous Brietal (methohexitone): the findings in 100 cases. One hundred cases of methohexitone activation of the EEG are reported. The technique seems to be especially valuable in the investigation of petit mal and temporal lobe epilepsy and adds valuable negative evidence in cases of suspected behaviour disorders.


Blood flow currents in spinal cord arteries.


Neurologic complications of malignant external otitis.


Ultrasonic measurement of cerebral ventricular system. A critical and comparative study of echoencephalographic and pneumoencephalographic determinations in 100 patients.


Primary Pseudomonas pneumonia.

Primary Pseudomonas pneumonia.


The role of betahistine hydrochloride in the treatment of peripheral vascular arterial disease.


Heterochromatin pattern in metaphase chromosomes of Drosophila melanogaster.


A ten-year reevaluation. Nitroglycerin and isosorbide dinitrate in coronary insufficiency.


Are you a painful dentist?

Are you a painful dentist?


Annual directory membership roster--Arkansas State Dental Association.


The serologic diagnosis of extracutaneous sporotrichosis.


Effect of modification on physiochemical and biological properties of haptoglobin. 3. Antigenic reactivity of haptoglobin with blocked tyrosine or tryptophan residues.


Prothrombal: a new concentrate of human prothrombin complex for clinical use.


Haemolysis and intravascular coagulation due to incompatible red cell transfusion in isoimmunized monkeys.


Advances in obstetric anesthesiology during the period 1960-1970.


Electrophoretic patterns of red-cell membrane protein from various mammalian species.


Intravenous anesthesia.

Intravenous anesthesia.


Animal carbonic anhydrase isozymes.

Animal carbonic anhydrase isozymes.


Evolution of hemoglobin in primates and other therian mammals.


Amino acid patterns in the plasma of old and new world primates.


Studies on the minor haemoglobin components of anthropoid apes and new world monkeys.


Hexose-ATP phosphotransferases: comparative aspects. II. Cross-reactivity of hexokinases from a variety of species with antisera to yeast hexokinase.


The lipophilic character of carboxy derivatives of penicillins, and its relation to their antibacterial activities.


Diseases of the heart in wild rhesus monkeys.


Ribosome patterns in Escherichia coli growing at various rates. The distribution of ribosomes, 30 and 50S subunits and polysomes, at three different growth rates of Escherichia coli strains B and K-12 has been studied. The usual percentage of subunits is about 20%. However, at the lowest growth rate (mu = generations/hour), mu = 0.45 at 30C, the proportion of subunits is about 30%. An exceptional situation exists in K-12 strains growing at maximum growth rate, mu = 1.35, where the percentage of subunits is 45%. Several points of control over ribosome production are thus indicated. It is suggested that "subunit pool" is essentially a reserve. Furthermore, the polysome content when related to deoxyribonucleic acid content varies directly with the growth rate, which indicates the average efficiency of polysomes in protein synthesis does not vary over the range of growth rates tested.


Distribution and correlation of events during thermal inactivation of Bacillus megaterium spores. Aqueous suspensions of Bacillus megaterium QM B1551 spores were heated at temperatures from 75 to 85 C. The rapid initial viability loss, followed by a more gradual, almost exponential decline, was not due to mixed populations with discrete heat resistances. The slight "tailing" below 0.01% survival was not the result of heat adaptation. Loss of viability was more rapid than loss of dipicolinic acid (DPA) and germinability and, although these events could not be correlated by use of simple kinetic plots, they had similar activation energies (80 to 90 kcal/mole). Probability (probit) plots of per cent survival as a function of logarithmic time yielded not the single line expected, if the heat resistances of individuals in the population were log-normally distributed, but two straight lines intersecting at a survival level of 1 to 6%. Probit-intersects occurred at times ranging from 8 min for spores heated at 85 C, to 310 min at 75 C. Probit-intersects for DPA release and loss in germinability occurred at the same time as for survival, but at much higher levels of retention. There appeared to be two subpopulations, both log-normally distributed but with different mechanisms of kill. Ninety-four to 99% of the spores died via injury to the cell-division process but retained germinability; the remaining smaller subpopulation (1 to 6%) was nonviable because of loss of the ability to germinate.


Formation of  -cyanoalanine by O-acetylserine sulfhydrylase. Cell-free extracts of Bacillus megaterium form beta-cyanoalanine (beta-CNA)-(14)C from Na(14)CN and l-cysteine, O-acetyl-l-serine or, to a lesser extent, l-serine. However, the presence of cyanide in the growth medium does not increase the capacity of cell extracts to catalyze the formation of beta-CNA from cysteine and cyanide. The formation of beta-CNA is readily detected in extracts of cells grown in synthetic media with sulfate or l-djenkolic acid as sulfur sources; such cells also exhibit an increased ability to form cysteine when compared with cells grown on cysteine as the sulfur source. beta-CNA formation could not be detected in extracts of cells grown on cysteine as the sulfur source. A 40-fold purification of the O-acetyl-serine sulfhydrylase resulted in the co-purification of the beta-CNA-forming activity. The sulfhydrylase and the beta-CNA-forming activity co-chromatographed on diethyl-aminoethyl cellulose and Sephadex G-100.


Hyphal wall synthesis in Aspergillus nidulans: effect of protein synthesis inhibition and osmotic shock on chitin insertion and morphogenesis. Pulse-labeling with N-[acetyl-(3)H] glucosamine and radioautography were used to follow the sites of chitin incorporation in hyphae of an Aspergillus nidulans mutant blocked in amino sugar synthesis. Growing hyphae incorporated N-acetylglucosamine almost exclusively at the tip. Cycloheximide addition greatly increased the label in subapical regions of the hyphae and reduced that at the tip. This effect of cycloheximide was immediate, could be reversed by removing the inhibitor, and did not appear to be due to chitin turnover. A similar change from apical to subapical N-acetylglucosamine incorporation occurred after hyphae were subjected to an osmotic shock which did not inhibit protein synthesis. The two treatments induced morphogenetic changes in the hyphae which produced abnormally large numbers of branches and septa.


Detection of relationships between Streptococcus faecalis and Lactobacillus casei by immunological studies with two forms of malic enzyme. Antisera prepared against two isologous malic enzymes from Lactobacillus casei strains 64H and M40 were used to survey and categorize the various malic enzymes found within this diverse species. In addition to detecting three major antigenic variants of malic enzyme within this group, both antisera readily reacted with Streptococcus faecalis malic enzyme. The cross-reactions between the L. casei malic enzyme antisera and the S. faecalis malic enzyme indicated that these iso-functional enzymes found in two apparently diverse groups of organisms were immunologically homologous. A scheme proposing a common ancestry for the two species S. faecalis and L. casei based on the results of quantitative immunological studies is presented.


Mutagenic action, loss of transforming activity, and inhibition of deoxyribonucleic acid template activity in vitro caused by chemical linkage of carcinogenic polycyclic hydrocarbons to deoxyribonucleic acid. In a solvent system of 10(-2)m phosphate buffer (pH 6.8)-ethanol (2:1, v/v) and in an iodine-induced reaction, the polycyclic hydrocarbons [(3)H]3,4-benzpyrene and [(3)H]3,4-BP/[(3)H]9, 10-dimethyl-1,2-benzanthracene (DMBA) can be covalently linked to deoxyribonucleic acid (DNA) at room temperature. By stepwise addition of the hydrocarbon and repeating the reaction two to three times after isolating the hydrocarbon DNA adduct, it was possible to introduce as many as one covalently bound hydrocarbon molecule per 100 nucleotide bases. When 3,4-BP and DMBA were linked in this way to biologically active transforming DNA of Bacillus subtilis, they caused (i) reduction of the transforming activity of the DNA accompanied by (ii) significant increases in the frequency of forward mutations. The majority of these hydrocarbon-induced mutations were not able to revert spontaneously. These samples of DNA covalently linked with hydrocarbons showed much lower levels of survival of biological activity when assayed in recipient strains (hcr(-)) which are known to be deficient in the enzymes required for repair of ultraviolet light-induced damage to DNA. 3,4-BP covalently linked to calf thymus DNA at a level of approximately one hydrocarbon molecule per 330 bases was shown to cause up to 80% inhibition of the in vitro transcription of the DNA by highly purified ribonucleic acid polymerase prepared from Micrococcus luteus under the experimental condition of template saturation. The presence of 3,4-BP and DMBA molecules covalently bound to B. subtilis DNA samples was also found to prevent complete denaturation of the bihelical structure of certain DNA molecules and thus appears to effect a cross-link in these DNA molecules.


Early intermediate state of transforming deoxyribonucleic acid during uptake by Bacillus subtilis. Examination of the effects of ethylenediaminetetraacetic acid (EDTA) on uptake of transforming deoxyribonucleic acid (DNA) by Bacillus subtilis allowed definition of a new intermediate state of transforming DNA during uptake. Markers in this state, termed "EDTA-resistant," are bound to the cell, are sensitive to inactivation by deoxyribonuclease, but may become deoxyribonuclease-resistant despite the addition of excess EDTA. Markers become EDTA-resistant quadratically with time after the addition of DNA to a competent culture, but linearly after exposure of competent cells to a brief pulse of DNA. An attractive model consistent with these findings is that EDTA blocks only the initiation of entry of a molecule of DNA.


In vivo and in vitro action of new antibiotics interfering with the utilization of N-acetyl-glucosamine-N-acetyl-muramyl-pentapeptide. Recent literature on the antibiotics enduracidin, moenomycin, prasinomycin, and 11.837 RP suggested an interaction with murein synthesis. Incubation of sensitive strains from Bacillus cereus and Staphylococcus aureus in a "wall medium" containing labeled l-alanine showed that all four antibiotics inhibited the incorporation of alanine into murein and gave rise to accumulation of radioactive uridine diphosphate-N-acetyl-muramyl (UDP-MurNAc)-pentapeptide. Peptidoglycan was synthesized when the particulate enzyme of B. stearothermophilus was incubated with the murein precursors UDP-N-acetyl-glucosamine (UDP-GlcNAc) and UDP-MurNAc-pentapeptide. The newly formed polymer was less accessible for lysozyme and more strongly bound to the acceptor than the same product from the Escherichia coli particulate enzyme. After incubation in the presence of penicillin, a greater part of the peptidoglycan was lysozyme sensitive and more loosely bound to the acceptor. The antibiotics enduracidin, moenomycin, prasinomycin, and 11.837 RP inhibited peptidoglycan synthesis by the B. stearothermophilus particulate enzyme. The rate of synthesis of GlcNAc-MurNAc(-pentapeptide)-P-P-phospholipid was independent from the addition of these antibiotics, but its utilization was strongly inhibited. With the present results, it is not possible to distinguish the mechanisms of action of enduracidin, moenomycin, prasinomycin, and 11.837 RP from the mechanisms of action of vancomycin and ristocetin.


Proposal for classifying human strain navel and related simian mycoplasmas as Mycoplasma primatum sp. n. Mycoplasmas isolated from simian (Cercopithecus aethiops) tissues were shown to have biological, biochemical, and serological properties and electrophoretic cell protein patterns similar to strain Navel isolated from man 15 years ago. The simian and human Navel strains comprised a single serogroup, distinct from the established Mycoplasma and Acholeplasma species of the class Mollicutes. It is proposed that strains with the properties described be named Mycoplasma primatum.


Antimicrobial actions of hexachlorophene: lysis and fixation of bacterial protoplasts. Hexachlorophene was found to be both a lytic and a fixative agent for protoplasts isolated from Bacillus megaterium. Concentrations of 50 to 100 mug of drug per mg of original cell dry weight were required to lyse 4.4 x 10(9) protoplasts (2 mg of original cell dry weight). At higher drug concentrations, protoplasts became fixed against osmotic stress and reduced in sensitivity to disruption by n-butanol. Lower drug concentrations caused proportionate lysis in the protoplast population. Intact cells lost the ability to become plasmolyzed at these same hexachlorophene concentrations. Nonplasmolyzed, drug-treated cells were resistant to the action of lysozyme, whereas plasmolyzed, drug-treated cells were sensitive. But the sensitivity of isolated cell walls to lysozyme digestion was not markedly altered by hexachlorophene treatment. These effects appeared to be secondary in the killing of cells by hexachlorophene because they occurred at concentrations higher than the minimum lethal concentration.


Cold osmotic shock in Saccharomyces cerevisiae. Saccharomyces cerevisiae NCYC 366 is susceptible to cold osmotic shock. Exponentially growing cells from batch cultures grown in defined medium at 30 C, after being suspended in 0.8 m mannitol containing 10 mm ethylenedia-minetetraacetic acid and then resuspended in ice-cold 0.5 mm MgCl(2), accumulated the nonmetabolizable solutes d-glucosamine-hydrochloride and 2-aminoisobutyrate at slower rates than unshocked cells; shocked cells retained their viability. Storage of unshocked batch-grown cells in buffer at 10 C led to an increase in ability to accumulate glucosamine, and further experiments were confined to cells grown in a chemostat under conditions of glucose limitation, thereby obviating the need for storing cells before use. A study was made of the effect of the different stages in the cold osmotic shock procedure, including the osmotic stress, the chelating agent, and the cold Mg(2+)-containing diluent, on viability and solute-accumulating ability. Growth of shocked cells in defined medium resembled that of unshocked cells; however, in malt extract-yeast extract-glucose-peptone medium, the shocked cells had a longer lag phase of growth and initially grew at a slower rate. Cold osmotic shock caused the release of low-molecular-weight compounds and about 6 to 8% of the cell protein. Neither the cell envelope enzymes, invertase, acid phosphatase and l-leucine-beta-naphthylamidase, nor the cytoplasmic enzyme, alkaline phosphatase, were released when yeast cells were subjected to cold osmotic shock.


Microstructure of colonies of rod-shaped bacteria. Whole colonies of Bacillus cereus, B. megaterium, B. mycoides CN2495, Corynebacterium hofmanni NCTC1938, Escherichia coli, Lactobacillus acidophilus NCIB1899, Nocardia graminis NCTC4728, Pseudomonas viscosa, and Serratia marcescens were prepared for scanning electron microscopic examination by freeze-drying and metal-coating. The arrangement of individual cells within colonies could be seen. Cells of Bacillus colonies tended to be longer than in liquid culture and irregular in shape and to give the appearance of branching. B. megaterium colonies frequently had a dense covering film. Colonies of gram-negative bacteria consisted of fairly short rods covered by much adherent extracellular material. L. acidophilus had colonies comprised of densely packed, well-oriented rods. C. hofmanni colonies contained coccobacilli, packed together. Correlations were observed between plano-convex colony form and densely packed cells, rough colony form and random arrangement of well-separated microorganisms, and irregular colony edge and tendency of cells to grow out from the colony in filaments.


Reconstitution of bacterial spore coat layers in vitro. The spore coat layers of Bacillus cereus T can be at least partially restored by mixing spores stripped of their coat with solubilized coat protein.


Electron microscopy of tissue culture cells infected with Brucella abortus. Thin sections of hamster kidney tissue cultures were examined by electron microscopy over a 7-day period after infection with Brucella abortus 3183. Numerous bacteria and structures resembling L-forms were present both intracellularly and extracellularly after the first 24 hr of infection. Most intracellular microorganisms were enclosed by a cytoplasmic membrane, but in a few instances no limiting membrane was detected. After 4 to 7 days, fewer microorganisms were present, and most normal-appearing bacteria were intracellular, particularly in antibiotic-treated cultures. Structures typical of Brucella L-forms were extracellular at the latter time intervals. Several structures were observed in cells from infected cultures whose relationship to the infecting organisms is not known. These consisted of various membranous structures within cytoplasmic vacuoles, myelin-like structures surrounding occasional intracellular organisms, and small bodies present within vacuoles and extracellularly. The latter structures observed throughout the experimental period appeared to occur more frequently as the duration of the infection increased.


Effect of ethylenediaminetetraacetic acid, Triton X-100, and lysozyme on the morphology and chemical composition of isolate cell walls of Escherichia coli. Extraction of a partially purified preparation of cell walls from Escherichia coli with the nonionic detergent Triton X-100 removed all cytoplasmic membrane contamination but did not affect the normal morphology of the cell wall. This Triton-treated preparation, termed the "Triton-insoluble cell wall," contained all of the protein of the cell wall but only about half of the lipopolysaccharide and one-third of the phospholipid of the cell wall. This Triton-insoluble cell wall preparation was used as a starting material in an investigation of several further treatments. Reextraction of the Triton-insoluble cell wall with either Triton X-100 or ethylenediaminetetraacetic acid (EDTA) caused no further solubilization of protein. However, when the Triton-insoluble cell wall was extracted with a combination of Triton X-100 and EDTA, about half of the protein and all of the remaining lipopolysaccharide and phospholipid were solubilized. The material which remained insoluble after this combined Triton and EDTA extraction still retained some of the morphological features of the intact cell wall. Treatment of the Triton-insoluble cell wall with lysozyme resulted in a destruction of the peptidoglycan layer as seen in the electron microscope and in a release of diaminopimelic acid from the cell wall but did not solubilize any cell wall protein. Extraction of this lysozyme-treated preparation with a combination of Triton X-100 and EDTA again solubilized about half of the cell wall protein but resulted in a drastic change in the morphology of the Triton-EDTA-insoluble material. After this treatment, the insoluble material formed lamellar structures. These results are interpreted in terms of the types of noncovalent bonds involved in maintaining the organized structure of the cell wall and suggest that the main forces involved are hydrophobic protein-protein interactions between the cell wall proteins and to a lesser degree a stabilization of protein-protein and protein-lipopolysaccharide interactions by divalent cations. A model for the structure of the E. coli cell wall is presented.


Fiber-structured material in a fraction of Bacillus subtilis. The paracrystal fractions of two species of Bacillus contain similarly structured fibrous material whose unit cell is hexagonal, with cell dimensions of a = 0.719 nm and c = 0.963 nm.


Regulation of tyrosyl-transfer ribonucleic acid synthetase in Bacillus subtilis. Derepression of tyrosyl-transfer ribonucleic acid synthetase in Bacillus subtilis was observed in strains grown with limiting tyrosine, but not in regulatory mutants derepressed in biosynthetic enzyme synthesis.


Chemotactic response to amino acids by Pseudomonas aeruginosa in a semisolid nitrate medium. Pseudomonas aeruginosa exhibited positive chemotaxis to glutamate, alanine, and arginine in a nutrient semisolid nitrate medium.


Involvement of sulfhydryl groups in the  -galactosidase of Streptococcus lactis. Sulfhydryl oxidants and stabilizers caused changes demonstrating the sulfhydryl content of beta-galactosidase for Streptococcus lactis 7962. Ammonium sulfate (0.85 m) rendered the enzyme insensitive to the oxidants. Titrations revealed 11.5 moles of sulfhydryl per mole of enzyme.


Transport of glycerol by Pseudomonas aeruginosa. In Pseudomonas aeruginosa, the transport of glycerol was shown to be genetically controlled and to be dependent on induction by glycerol. Accumulation of (14)C-glycerol was almost completely absent in uninduced cells and in a transport-negative mutant. Kinetic studies with induced cells suggested that glycerol may be transported by two systems with different affinities for glycerol. Osmotically shocked cells did not transport glycerol, and the supernatant fluid from shocked cells contained glycerol-binding activity demonstrable by equilibrium dialysis. The binding protein was not glycerol kinase. Binding activity was absent in shock fluids from the transport-negative mutant and from uninduced cells. The glycerol-binding protein was partially purified by precipitation with ammonium sulfate. Mild heat treatment completely eliminated the binding activity of shock fluid and of the partially purified protein. Sodium azide and N-ethylmaleimide inhibited both transport by whole cells and binding of glycerol by shock fluid. It is concluded that transport of glycerol by P. aeruginosa involves a binding protein responsible for recognition of glycerol and may occur by facilitated diffusion or active transport. A requirement for energy has not been demonstrated.


Autogenous transplants.

Autogenous transplants.


Roentgen, discoverer of the xray.

Roentgen, discoverer of the xray.


Isolation, mapping, and characterization of trehalaseless mutants of Neurospora crassa. Mutant strains of Neurospora crassa that lack trehalase and are unable to grow on trehalose were isolated, and the gene (tre) was positioned on the right arm of linkage group I. Maltase and beta-galactosidase activities are almost identical in tre(-) strains, whereas that of invertase was reduced by more than half and those of acid phosphatase and amylase were somewhat increased. Heterocaryons between standard and trehalaseless strains yield less than one-tenth the activity of the former. In addition, strains with duplications heterozygous for trehalase produce less than 1% of the activity of the standard strain. An inhibitor of trehalase has been found in tre(-) strains; its sensitivity to heat and proteolysis, and its nondialyzability suggest that this substance is a protein. The mig gene, which determines the rate of migration of trehalase on acrylamide gels, has been shown to be less than 1 map unit away from the tre gene.


Response of noraml, hypothyroid and hypothalamo-pituitary insufficient children to synthetic thyrotrophin-releasing hormone.


The effects of dexamethasone and androgens on sexual receptivity of female rhesus monkeys.


Studies on a dynamic cation transport in the rat submaxillary gland by carbonic anhydrase inhibitors.


Hospitals in Sweden. New systems, new buildings, new equipment, and old problems.


High blood pressure and epilepsy in hydrocephalus due to stenosis of the aqueduct of Sylvius.


Treatment of pseudarthrosis involving the malleolus. A postoperative follow-up of 34 cases.


Hemoglobins in Macaca speciosa: quantitative differences.


Marine bacterial proteases. I. Characterization of an endopeptidase produced by Vibrio B-30.


Biochemical aspects of the visual process. 13. The role of phospholipids in cattle rhodopsin studied with phospholipase C.


Polypeptides minimine and melittin from bee venom: effects on Drosophila.


The rate of electron transfer between fungal laccase and reduced azurin or cytochrome c.


Melittin: toxicity to Drosophila and inhibition of acetylcholinesterase.


Interaction between glycogen and glycogen phosphorylase of Tetrahymena.


Purification and properties of butyrylcholinesterase from horse serum.


Degradation of RNA in rat reticulocytes. I. Purification and properties of rat reticulocyte RNase.


Studies on the reductive cleavage of canavanine and canavaninosuccinic acid.


Nitrous oxide and oxygen.

Nitrous oxide and oxygen.


Composition and carboxyl-terminal amino acid sequences of some mammalian erythrocyte carbonic anhydrases.


Fetal hepatic function.

Fetal hepatic function.


Photodecay of bilirubin in vitro and in the jaundiced (Gunn) rat.


On the organization of central visual pathways in vertebrates.


Behavioral changes following midline section of the mesencephalic tegmentum in the cat and monkey.


What the frog's eye tells the monkey's brain.


Subcortical vision: a prologue.

Subcortical vision: a prologue.


Rheumatoid factor in the United Kingdom. II. Associations with certain infections.


Secretory IgA in seminal fluid.

Secretory IgA in seminal fluid.


Introduction to discussion of glucose-6-phosphate dehydrogenase deficiency.


Immunoglobulins, virus-like particles and autoantibodies in bile.


Inhibition of blood platelet aggregation by serum IgM macroglobulin. Preliminary report.


Carbohydrate-induced hyperlipemia in an infant.


B-like blood factor of fur seals and sea lions. Comparative study with D-galactose inhibition, human group B erythrocytes and B-active human, baboon and rhesus monkey saliva.


An a particle, probably of bovine origin, detected in BHK21 cells infected with bovine syncytial virus.


Comparison of interferon induction in mice by purified Penicillium chrysogenum virus and derived double-stranded RNA.


The morphogenesis and classification of bovine syncytial virus.


Melanization and tyrosinase activity.

Melanization and tyrosinase activity.


Induction of tumors in marmoset monkeys by simian sarcoma virus, type 1 (Lagothrix): a preliminary report.


Oncogenicity of herpesvirus saimiri in marmoset monkeys.


Antitubercular substances. XXII. Rimino-phenazines--the effect of further substitution.


DNA constancy in heteroploidy and the stem line theory of tumors. Cellular DNA was measured by high-speed flow microfluorometry in mammalian diploid and heteroploid cell populations stained by the fluorescent Feulgen procedure. Heteroploid cells with elevated modal chromosome number showed the expected increase in modal DNA content. However, the variability of DNA content was the same in diploid and heteroploid cell populations despite the large variability of chromosome number in the latter populations. This suggests that heteroploidy may include defects in the chromosomal condensation and kinetochore development systems.


Radiation and wound healing: evolution of tensile strength in excised skin-wound of irradiated rats.


A comparison between phenol red and  51 Cr-EDTA as reference substances in rats by a test meal technique.


Studies on the conidia of Aspergillus oryzae. XI. Latent ribonuclease in the conidia of Aspergillus oryzae.


Intracellular localization of ribosome biosynthesis in osmotically fragile forms of Streptococcus faecalis.


An interactive digital computer system for radiotherapy treatment planning.


Duodenal obstruction--an unusual complication of a gastrostomy feeding catheter.


An in vivo dosimeter for fast neutrons.

An in vivo dosimeter for fast neutrons.


Surface effects of high-energy x-rays at oblique incidence.


Immunological studies on treponemal antigens. I. Isolation and characterization of an antigenic substance in the culture supernatant of avirulent T. pallidum (Nichols).


Treatment of gonorrhoea with a single oral dose of minocycline.


Penicillin-insensitive gonococci in the Bolton area. Preponderance in young women and immigrants.


Serological diagnosis in gonorrhoea.

Serological diagnosis in gonorrhoea.


The current state of treatment of gonorrhoea with reference to decreased penicillin sensitivity of Neisseria gonorrhoeae.


Adsorption of polychlorinated biphenyls (PCB) on glass surfaces.


Threonine deaminase (dehydratase) in Azotobacter chroococcum.


New liquid-diet feeder for primates.

New liquid-diet feeder for primates.


Effect of some hydrocarbon gases on egg-white lysozyme activities on different substrates.


Incidence of Rh iso-immunisation in pregnant women and erythroblastosis foetalis.


Studies on Dracunculus medinensis (Linnaeus). II. Effect of acidity on the infective larva.


Automated behavioral conditioning in animals.


Platelet adhesiveness and serum lipids during and after Intralipid infusions.


Carriers of meningococci among Norwegian naval recruits.


Hepatitis-associated antigen (HAA) in acute viral hepatitis. Serological and clinical studies.


6 -hydroxy-  1 -tetrahydrocannabinol synthesis and biological activity. 6beta-Hydroxy-delta(1)-tetrahydrocannabinol, a metabolite of delta(1)-tetrahydrocannabinol has been synthesized from delta(6)-tetrahydrocannabinol. It shows high tetrahydrocannabinol-type activity in rhesus monkeys. The implications of this funding are discussed.


A comparison of serum PBI and thyroxine iodine levels.


Brain excitability and behavioral reactivity in monkeys under meprobamate.


Autoradiographic study of the fate of diazepam-C 14  in the monkey brain.


Isolation of normal immunoglobulin E by means of an immunoadsorbent. An improved method for the isolation of normal immunoglobulin E is described. The method is based on the use of an immunoadsorbent formed by the mechanical entrapment of antibodies against a myeloma immunoglobulin E into a lattice of a highly cross-linked macroporous polyacrylamide gel. Normal immunoglobulin E was isolated from an immunoglobulin E-rich serum pool as well as from an individual healthy donor. The isolated immunoglobulin E was contaminated with about 20% of immunoglobulin G. An antiserum against normal immunoglobulin E was prepared by immunizing rabbits with the isolated immunoglobulin E.


Relationship between alkaline phosphatase and neomycin formation in Streptomyces fradiae. Studies on phosphatase activity of Streptomyces fradiae 3535 grown in three different media indicate that neomycin formation varies directly with enzyme activity, sodium nitrate-maltose-mineral salts medium giving the highest yields of alkaline phosphatase and neomycin. S. fradiae contains more than one alkaline phosphatase and the phosphatase responsible for hydrolysis of neomycin phosphate appears to be substrate specific. The same enzyme apparently hydrolyses both the N-P and P-O-P bonds of neomycin pyrophosphate. The enzyme is stimulated by Ca(2+), is inactive at a pH below 7 and is inhibited by EDTA. Enzymic activity increases when mycelia are incubated in mineral salts medium, but decreases when phosphate or glucose is included in the medium, although the latter is more effective. The inhibitory effect of EDTA on neomycin formation by resting mycelia is completely reversed by Ca(2+).


Synthesis and oxidation of aminoalkyl-onium compounds by pig kidney diamine oxidase. 1. The preparation of a series of compounds derived from diamines by replacing one amino group by a dimethylsulphonium, isothiuronium, trimethylammonium, NN'-dimethylimidazolium or N-methylpyridinium species is described. 2. The behaviour of these compounds as substrates of pig kidney diamine oxidase is reported. All but the trimethylammonium compounds proved to be substrates. 3. Many of these compounds showed potent inhibition at high substrate concentration and this was studied. 4. On the basis of these and other observations a scheme for enzyme-substrate interaction is suggested.


Nonpolar effects in reactions of the sulfhydryl group of papain.


Further characterization of the apolipoproteins of rat plasma lipoproteins.


Purification and properties of cytochrome C 3 of Desulfovibrio salexigens.


Listeriosis.

Listeriosis.


Alcohol and glibenclamide.

Alcohol and glibenclamide.


Vasodilators in menière's syndrome.

Vasodilators in menière's syndrome.


Thyreotropin-releasing hormone and serum levels of H-TSH, HGH, thyroxine, insulin and glucose.


Feto-maternal transfusion. A study of 100 pregnancies.


The effects of 4-acetamidophenol (paracetamol) on the temperature response of the conscious rat to the intracerebral injection of prostaglandin E, adrenaline and pyrogen.


Monitoring mouse stocks for lymphocytic chloriomeningitis virus--a human pathogen.


Aflatoxin, monkeys, and humans.

Aflatoxin, monkeys, and humans.


Centrifugal control of gustatory responses.


Recording the electrocardiogram from the squirrel monkey, Saimira sciureus.


Urinary 17-OHCS response of high- and low-aggressive rhesus monkeys to shock avoidance.


Effects of food deprivation on free-operant avoidance.


Cytotoxic effect of Mycobacterium tuberculosis on J-111 cells.


Myopathy with mitochondrial inclusions.

Myopathy with mitochondrial inclusions.


Relationships among three isolates of barley yellow dwarf virus.


Mitochondrial ultrastructure and energy transduction in rat heart during progressive thiamine deficiency.


Quantitation of vibriocidal antibodies using agar plague techniques.


Coronary dynamics in unrestrained conscious baboons.


Kinetic localization of contractile calcium in rabbit myocardium.


Vasodilators in Menière's syndrome.

Vasodilators in Menière's syndrome.


The stimulatory effects of carbon tetrachloride on peroxidative reactions in rat liver fractions in vitro. Inhibitory effects of free-radical scavengers and other agents. 1. The effects of a number of free-radical scavengers and other agents on the stimulation of malonaldehyde production due to low concentrations of carbon tetrachloride have been studied in rat liver microsome suspensions. 2. Promethazine, propyl gallate and NN'-diphenyl-p-phenylenediamine were extremely active in inhibiting the stimulation of malonaldehyde production due to carbon tetrachloride; inhibitory effects were demonstrable with these agents at 0.1mum. 3. Low concentrations (1-100nm) of vitamin E-polyethylene glycol 1000-succinate increased the stimulation of malonaldehyde production due to carbon tetrachloride, but higher concentrations of the vitamin E preparation decreased both the stimulation due to carbon tetrachloride and the endogenous peroxidation that occurs in the absence of carbon tetrachloride. 4. Other agents tested that were effective in the range 1-20mum in decreasing the stimulation of malonaldehyde production due to carbon tetrachloride were inosine, desferrioxamine and EDTA. Agents tested that were not effective, except at very high concentrations (100mum or greater), were Nupercaine, Cetab and sodium phenobarbitone. 5. The results are discussed in terms of the mechanisms responsible for the observed inhibitions of malonaldehyde production, and of the relevance of the in vitro system to the liver damage produced by carbon tetrachloride in vivo.


Disinhibition during waking in motor cortex neuronal chains in cat and monkey.


Some characteristics of placental alkaline phosphatase of a variety of mammals.


Increased proteolytic activity in muscular atrophy of denervated frogs.


DNA replication sequence in Tetrahymena is not repeated from generation to generation.


Heterochromatin location on chromosomes of normal and transformed cells from African green monkey (Cercopithecus aethiops). DNA denaturation-renaturation method.


Mechanism of synchrony induction. I. Some features of synchronous rounding in Tetrahymena pyriformis.


Mechanism of synchrony induction. II. Synthesis and turnover of biomolecules in the induction process of synchronous rounding in Tetrahymena pyriformis.


Divalent cation-mediated epithelial union with connective tissue.


Action of concanavalin A on the attachment stage of phagocytosis by macrophages.


A late replicating DNA protein complex from cells in meiotic prophase.


Influence of culture pH and phosphate on synchrony of Euglena gracilis.


Protein synthesis on polysomes in mitochondria isolated from Euglena gracilis.


Proliferation and specific function of neoplastic mast cells in culture. Effects of amino acid deprivation.


Immunological comparisons of histidinol dehydrogenases.


The haemoglobins of healthy and anaemic Xenopus laevis.


The effect of different ionic levels on the electrical response of toad skin to noradrenaline. 1. After the addition of noradrenaline (final concentration 4 x 10(-5)M) to the inner medium of isolated toad skins, they underwent a depolarization (inner surface becoming less positive) followed by a hyperpolarization.2. The dependence of the minimal and maximal levels of the depolarizing and hyperpolarizing phases of the response upon the external concentrations of sodium and chloride ions was examined.3. The experimental data were considered to support the view that the hyperpolarization was generated by an increase in the sodium to chloride permeability ratio for the outer barrier of the skin and that the depolarization resulted from a transient increase in the conductance of transient shunt pathways in the skin.4. When the external sodium and chloride ions were replaced by magnesium (or potassium) and sulphate ions, noradrenaline initiated a transient hyperpolarization. During this hyperpolarizing response the time course of the change in the skin's conductance resembled that of the skin potential. The polarity of the response was changed by reversing the chloride concentration gradient across the skin.5. The dependence of the maximal level of the hyperpolarizing response upon the internal potassium concentration was examined.6. It was concluded that the hyperpolarizing response was generated partially by an increase in the potassium to chloride permeability ratio for the inner barrier of the skin and predominantly by the movement of chloride and accompanying cations through a transient shunt pathway, probably the active glands.


An investigation of a possible memory defect produced by inferotemporal lesions in the baboon.


Delayed alternation in monkeys after removal of the hippocampus.


Cross-modal transfer of serial reversal learning in the monkey.


Electrically-induced afterdischarges in the inferotemporal cortex of monkeys: effects on visual discrimination and discrimination-reversal performance.


Retrograde amnestic effects of inferotemporal and amygdaloid seizures upon conditioned suppression of lever-pressing in monkeys.


Cross-modal transfer between touch and vision without change of illumination.


Flicker responses in monkey lateral geniculate nucleus and human perception of flicker. An analysis was made of the impulse discharge patterns-evoked by sinusoidal luminance modulation-of single cells in the lateral geniculate nucleus of the macaque monkey. The goal was to determine whether a correspondence could be observed between flicker detection by human subjects in psychophysical experiments and electrophysiological measurements of discharge patterns of single cells of the lateral geniculate nucleus. We found that the average discharge patterns of single cells exhibited the following behavior when mean retinal illumination was changed: In the low-frequency region (less than about 10 Hz) the response strength (impulses/sec) is independent of the mean luminance, in accordance with Weber's law; in the high-frequency region (above about 10 Hz) the response depends on the absolute modulation amplitude, in accordance with the Ferry-Porter law. Therefore the main features of human critical flicker frequency data are already present in the cellular (lateral geniculate nucleus) response of the macaque monkey. However, the steepness of high frequency fall-off in the response characteristics of these cells is much less than the corresponding fall-off in the human critical-flicker-frequency curves.


Lactobacillus exonuclease mutants: selection of mutants with altered ability to utilize deoxyribonucleotides. Lactobacillus acidophilus R-26, which requires preformed deoxyriboside for its growth, is able to grow in a basal medium supplemented with nitrophenyl esters of thymidine 3'- and 5'-phosphates. Mutants unable to grow on the thymidine phosphate esters were isolated and classified thus far into two classes. One class of mutants that are unable to grow on the diesters have modified exonucleases, their extracts being unable to hydrolyze the specific nitrophenyl ester on which the strain did not grow. It appears therefore, that the known 3'- and 5'-exonucleases specifically hydrolyzing these esters can be used for the support of growth. A second mutagenization treatment of one of the mutants gave rise to a new strain unable to use or hydrolyze either diester for growth, although it could still grow on several deoxyribodinucleotides. Another class of mutants does not grow on deoxyribooligonucleotides but grows on monodeoxyribo-nucleosides and -nucleotides. These mutants have normal levels of exonuclease activities, so it appears possible that they bear a mutation affecting permeability to diesters or to molecules larger than mononucleotides.


Anti-antibody activity of a monoclonal macroglobulin. A monoclonal macroglobulin from a patient with Waldenström macroglobulinemia has been shown to have antiglobulin activity of a new type, namely, that of a true anti-antibody. The protein reacts with human, primate, and rabbit IgM and IgG, only when the target immunoglobulin is complexed to an antigen. The implications of the general findings of antiglobulin activity of monoclonal proteins is discussed in relation to the possible etiology of the disease.


Observations on cell proliferation in human myelocytes.


Some data concerning the onset of the acute myeloblastic crisis in chronic myeloid leukaemia.


Treatment of acute leukaemia in adults with 6-mercaptopurine at high dosage.


Decreased erythrocyte deformability in cholestatic jaundice.


Study on the development of posthaemorrhagic thrombocytosis in rats.


Parahemophilia. A case report.

Parahemophilia. A case report.


Platelet defects in post-splenectomy thrombocythaemia.


An autopsy case of metachromatic leucodystrophy.


Radiation reactions in cervical cancer therapy.


Clinical and immunologic study of a direct agglutination test for pregnancy (mechanisms of interference including prozoning and circulating macroglobulins).


Epidermolysis bullosa dystrophica--anesthetic management.


Translation of genetic information on the ribosome.


The biosynthesis of tocopherols and related compounds in the blue-green alga Anabaena variabilis.


An assay for azo-reductase activity in micro-organisms.


Extraction of polymeric collagen from human skin.


A comparison of the effect of some currently available mixtures of amino--acids on postoperative metabolism.


The effects of prolonged cortical stimulation on the natural activity of neurones in the precentral gyrus of conscious monkeys.


Actions of the rifamycins.

Actions of the rifamycins.


The meaning for motoneurones of the temporal pattern of natural activity in pyramidal tract neurones of conscious monkeys.


Dendro-dendritic synapses, including reciprocal synapses, in the ventrolateral nucleus of the monkey thalamus.


Dendro-dendritic synapses in the primate motor cortex.


Cortical projections from two prestriate areas in the monkey.


The fate of axon terminals in visual cortex during trans-synaptic atrophy of the lateral geniculate nucleus.


Analysis of patterns of natural activity of neurones in the precentral gyrus of conscious monkeys.


Delayed alternation impairments following selective prefrontal cortical ablations in monkeys.


Changing hemisphere dominance by changing reward probability in split-brain monkeys.


Interaction of tooth pulp and periodontal ligament receptors in the dog and monkey.


Effectiveness of rifampin in eradicating the meningococcal carrier state in a relatively closed population: emergence of resistant strains.


What is bronchopulmonary dysplasia?

What is bronchopulmonary dysplasia?


Evaluation of resistance to staphylococcal enterotoxin B: naturally acquired antibodies of man and monkey.


Polyovular follicles in squirrel monkeys after prolonged diethylstilboestrol treatment.


Pregnenolone and progesterone concentrations in ovarian venous blood of the artificially ovulated squirrel monkey (Saimiri sciureus).


Immunosuppression: a means to assess the role of the immune response in acute virus infections.


Effect of gonadal hormones on electrical activity of brain in adult monkeys.


Light and electron microscope studies on the site of action of Enavid in the hypothalamus of the rhesus monkey.


Immunosuppression, interferon, and viral infections.


On the role of carbonic anhydrase in reproductive processes.


An immunochemical study of ovine pituitary follicle-stimulating hormone.


Recent advances in postoperative management of newborn infants.


Anticarcinogenesis--an alternative.

Anticarcinogenesis--an alternative.


Contamination of intravenous fluids by bacteria and fungi during preparation and administration.


Light and electron microscopic radioautography of lipids: techniques and biological applications.


Developments leading to the metabolic role of vitamin B 6.


Invasive aspergillosis. Absence of detectable antibody response.


Reduction of new tetrazolium salts containing thiophen ring by tubercle bacilli.


Clinical studies on rifampin: antituberculous activity and two cases of side effects.


Observations on the antibody response to Mycobacterium tuberculosis in laboratory animals.


The metabolism of D-mannose- 14 C to polysaccharide in corn roots. Specific labeling of L-galactose, D-mannose, and L-fucose.


Partial purification and enzymatic properties of an aminopeptidase from barley.


A comparison of the chemical composition of Mycobacterium tuberculosis muris with Mycobacterium tuberculosis bovis (BCG).


The mode of action of thiostreption in vivo.


Excretion of H +  and NH 4  +  by the urinary bladder of the acidotic toad and the effect of short-circuit current on the excretion.


Neurofibromatosis and childhood leukaemia.


Purification of an endogenous pyrogen, with an appendix on assay methods.


Quantitative determination of human fibrinogen in plasma following carbamylation by Laurell electrophoresis in antibody containing gel.


Identification of C-reactive protein by agarose gel electrophoresis of human serum or plasma.


Placental-type alkaline phosphatase from human tumour tissue.


The Rh problem.

The Rh problem.


Effect of amniotic fluid volume on prediction.


Immunochemical studies of group-specific agglutinins of diverse origin.


The vitamin K derivatives of some skin-mucins. I. Properties and vitamin K origin.


Thyroxine-binding prealbumin. Conformation in aqueous solutions.


The effects of pregnancy on circulating triiodothyronine.


Monilia septicemia associated with hyperalimentation.


Partial reinforcement: effects on visual learning after foveal prestriate and inferotemporal lesions.


Behavior after cerebellar lesions in cats and monkeys.


Isolation and chromosomal localization of highly repeated DNA sequences in Drosophila melanogaster. The nuclear DNA of D. melanogaster contains DNA sequences that are repeated between ten and a hundred times more often than the next class of redundant DNA. This DNA, as a renatured duplex isolated on the basis of its renaturation kinetics, has a buoyant density of 1.691 (g/ml). In its native state it bands within the unique nuclear DNA peak (rho = 1.701). These sequences have been localized by "in situ" hybridization in the chromocenter of the chromosomes of the salivary gland. The properties of centromeres are discussed in terms of the occurrence of repeated sequences at this locus.


Circular chloroplast DNA from Euglena gracilis. Chloroplast DNA of the protozoan flagellate, Euglena gracilis, exists as circular molecules, 40 mum in contour length, as shown by electron microscopy and buoyant density analyses.


Adenosine triphosphate-dependent synthesis of biologically active DNA by azide-poisoned bacteria. Nonviable cells of Bacillus subtilis, when made permeable by treatment under controlled conditions with nonionic detergent, can be shown to replicate DNA normally and to repair certain regions of the chromosome. The former process is stimulated by ATP in the presence of dATP, dCTP, dGTP, and dTTP. The product, a result of semiconservative replication, is biologically active; synthesis of the newly-formed regions of the chromosome appears to be sequential.


Temperature-sensitive mutations in Drosophila melanogaster. 8. The homeotic mutant, ss a40a . There exists in Drosophila a class of mutations called "homeotic" that cause changes in determination of the imaginal discs. The homeotic mutant, ss(a40a), was found to have a temperature-sensitive transformation of the arista segment of the antennal complex to a tarsus of the leg. In a selected stock penetrance was complete, so that at 29 degrees C normal aristae were produced, whereas at 17 degrees C complete tarsi developed in all flies. "Temperature-shift" studies revealed a temperature-sensitive period in the third larval instar. The temperature-initiated action of ss(a40a) does not appear to result from a simple wave or gradient of determination passing through the disc. Shifts up during the temperature-sensitive period yielded leg tissue proximally, which increased distally with progressively later shifts. Shifts down yielded leg tissue at the aristal base, which retreated with later shifts.


Radioisotope precipitation tests with  14 C and  51 Cr labeled vaccinia antigens.


Ethylenediaminetetraacetic acid and  65 Zn binding by intestinal digesta, intestinal mucosa and blood plasma.


Radioimmunoprecipitation assay for Australia antigen, antibody, and antigen-antibody complexes.


Relative hazards for inhaled 95 Zr and 95 Nb particles formed under various thermal conditions.


Localization of human blood to basophils.


Allergic encephalomyelitis: effect of complement depletion with cobra venom.


High incidence of adjuvant arthritis as a secondary lesion to peritonitis in rats.


Protein degradation at neutral pH. Possible enzymic and control mechanisms.


The effect of erythritol on the growth of S19 and other attenuated strains of Brucella abortus.


Perspectives in biological nitrogen fixation.


Chemical and immunological characterization of cucumber green mottle mosaic virus (watermelon strain) protein.


Amantadine hydrochloride inhibition of early and late stages of lymphocytic choriomenigitis virus-cell interactions.


Suppression of cellular DNA synthesis in growing cells by ultraviolet-irradiated adenoviruses.


Studies on lymphocyte kinetics in hematologically normal subjects.


A thermosensitive cyanophage (LPPI-G) attacking the blue-green alga Plectonema boryanum.


Pentose phosphate metabolism of erythrocytes in hepatic porphyrias.


Is haemoglobin G  Philadelphia linked to  -thalassaemia?


A study of  51 Cr-labelled platelets and the chromosomal pattern in a case of primary haemorrhagic thrombocythaemia.


X-radiation and the mandibular condyle.

X-radiation and the mandibular condyle.


Cell-mediated resistance to aerogenic infection of the lung.


On echolalia, echo-answer, and contamination.


The use of the gel diffusion precipitin technique with avian infectious bronchitis nephritis virus.


Perinatal lamb mortality: infections occurring among lambs dying after parturition.


Naevoxanthoendothelioma and neurofibromatosis.


Collagen content and collagen extractability pattern of adult human trabecular bone according to age, sex and amount of bone mass.


Clinical studies on influence of intravenous infusion of fat emulsion on changes in acid-base equilibrium.


Intravenous infusion of fat emulsion. Experimental and clinical studies on Intrafat.


Current concepts in the etiology and treatment of vasospasm.


Combination chemotherapy with L-asparaginase (EC-2) using P1798 lymphosarcoma.


The hazards of therapeutic iradiation of the central nervous system.


Phenylketonuria testing--its role in pediatrics and public health.


Mesenteric hemodynamics during endotoxemia in the baboon.


A two-dimensional coordinate model for the quantification, description, analysis, prediction and simulation of craniofacial growth.


Recovery from impulse-noise induced TTS in monkeys and men: a descriptive model.


Use of hyperalimentation in a community hospital.


[Antihypertensive treatment with a compound preparation (briserin)].


Treatment of acute iron poisoning.

Treatment of acute iron poisoning.


Control of thyrotropin secretion in man.


Nuclear vacuolization of the parafollicular cells of the thyroid gland in a curious disease of bovines.


Exercise-induced asthma.

Exercise-induced asthma.


Exercised-induced airways constriction.

Exercised-induced airways constriction.


Continuous assessment of the patient's condition during exercise induced bronchial obstruction.


Pelvic exenteration: a critical analysis of a ten-year experience with the use of the team approach.


The corticosteroid sparing effect of disodium cromoglycate in children and adolescents with bronchial asthma.


Cytology of tuberculin reaction.

Cytology of tuberculin reaction.


Induction of remission in myeloblastic and monoblastic leukaemia in adults with rubidomycin.


Clinical experiences with cytotoxic immunosuppressive treatment of idiopathic thrombocytopenic purpura.


The haematological response to the immunosuppressive methylhydrazine derivates procarbazine and Ro 4-6824.


The contribution of the intestinal mucosa to the total serum alkaline phosphatase activity.


Protective activity of some anti-anginal drugs on asphyxic anoxia of the brain; a contribution to their laboratory evaluation.


The development of perception as an adaptive process.


A study of the strength and stability of gas vesicles isolated from a blue-green alga.


The orbit.

The orbit.


Mucosal surface convolutions. Cellular aggregates observed on the enteric surface of various species.


The isolation of ovomucoid variants differing in carbohydrate composition. Three major and two minor species of ovomucoid were separated by chromatography on sulphoethyl-Sephadex. The predominant sialic acid-free species was further resolved into three fractions by DEAE-cellulose chromatography. Although all species of ovomucoid had closely similar trypsin-inhibiting activity, immunochemical properties and amino acid composition, they differ in carbohydrate composition. Wide variation was observed in the content of galactose, N-acetylglucosamine and sialic acid. Charge heterogeneity was related, in part, to variation in sialic acid content. The implications of variable carbohydrate composition for the structure and function of ovomucoid are discussed.


Purification and partial characterization of four proteins from human parotid saliva. Four proteins, which have been designated A, B, C and D, have been purified from human parotid saliva. These proteins are the major constituents of parotid saliva which migrate rapidly to the anode in polyacrylamide electrophoresis at pH9.5. Gel filtration and polyacrylamide electrophoresis were employed in the purification procedures. After purification all four preparations were tested for homogeneity by electrophoresis at pH2.8 and 9.5, by isoelectric focusing in the pH range 3-10, by immunodiffusion, and by sedimentation in the analytical ultracentrifuge. None of the proteins showed significant activity in assays for amylase, acid and alkaline phosphatase, protease, lysozyme, ribonuclease, peroxidase, beta-glucuronidase, beta-galactosidase, iron-binding activity and esterase. No cross-reactions were detected with antisera specific for lactoferrin and 15 serum proteins. All four proteins were rich in glutamic acid, proline and glycine and were lacking completely the sulphur-containing amino acids. Proteins A and C contained no threonine or tyrosine. Carbohydrate could be demonstrated only in protein A at a concentration of 4% of the total protein.


Quantitative angiography: the measurement of coronary vasomobility in the intact animal and man.


Hypotension during coronary arteriography.


Pulmonary tuberculosis with negative findings on chest x-ray films: a study of 40 cases.


Pattern formation and determination in the antenna of the homoeotic mutant Antennapedia of Drosophila melanogaster.


A mutant of bacteriophage T7 deficient in polynucleotide ligase.


Isolation and characterization of the immune macroglobulin from the paddlefish, Polyodon spathula.


Anthranilate synthetase. Some physical and kinetic properties of the enzyme from Serratia marcescens.


The dependence of immunological cross-reactivity upon sequence resemblance among lysozymes. II. Comparison of precipitin and micro-complement fixation results.


Nitration of a nitrophenyl-binding  M macroglobulin.


Separation of receptor and lamina potentials in the electroretinogram of normal and mutant Drosophila.


Coordination of the reproductive processes.


Titration and double responding as techniques promoting proficient learning in retardates.


Experimental ototoxicity of gentamicin in squirrel monkeys.


Microbiologic studies related to bacterial resistance to gentamicin.


Colonization with gentamicin-resistant Pseudomonas aeruginosa, pyocine type 5, in a burn unit.


Pseudomonas bacteremia: pharmacologic and other bases for failure of treatment with gentamicin.


Gentamicin: clinical use with carbenicillin and in-vitro studies with recent isolates of Pseudomonas aeruginosa.


Gentamicin for urinary-tract infections in infants.


Resistance of gram-negative bacilli to gentamicin.


Clinical and laboratory evaluation of gentamicin in infants and children.


Ecology of bacteria colonizing the burned patient given topical and systemic gentamicin therapy: a five-year study.


Gentamicin in gonococcal urethritis of Filipino males: dosage and response.


Activity of gentamicin against mycobacteria in vitro and against Mycobacterium tuberculosis in mice.


Effect of the concentrations of magnesium and calcium on the in-vitro susceptibility of Pseudomonas aeruginosa to gentamicin.


Effect of salt concentration on the apparent in-vitro susceptibility of Pseudomonas and other gram-negative bacilli to gentamicin.


Relation between dose and levels of gentamicin in blood.


Heptavalent pseudomonas vaccination in seriously burned children.


Satellite dialysis. An economic approach to the delivery of hemodialysis care.


Quinine as drug adulterant, and malaria transmission.


[Life-supporting machines. (Development of the cardiac pacemaker, ICU, and CCU)].


Pseudomonas aeruginosa in rabbit fur.

Pseudomonas aeruginosa in rabbit fur.


Design of cages and diet for metabolic studies using small New World primates.


The use of chemotherapy in lung cancer (controlled trials).


Sensitivity to ethambutol and to capreomycin in wild strains of Tubercle bacilli isolated from patients previously untreated with these drugs.


Two control mechanisms of growth and differentiation of the sympathetic nervous system.


Cytochemical, immunofluorescence and ultrastructural investigations on the antral G cells in hyperparathyroidism.


Plasma chromatography of the polychlorinated biphenyls.


Ossifying neurofibroma of the heart of a sheep.


Polysaccharides in germination. Xyloglucans ( amyloids') from the cotyledons of white mustard. Two xyloglucan fractions have been isolated from the cotyledons of resting white-mustard seeds, the first by extraction with hot EDTA, and the second by subsequent extraction with alkali or lithium thiocyanate. Although both appear to have the ;amyloid' type of structure in which chains of (1-->4)-linked beta-d-glucopyranose residues carry d-xylose-rich side chains through position 6, these side chains are rather different in structure in the two polysaccharide fractions, and the second or ;insoluble' xyloglucan has fewer of them. The side chains in both polysaccharides are also different from those in other seed amyloids, especially in having xylose linked through positions 3 and 4 (instead of through position 2 as usual) and in containing fucose residues. Both polysaccharides show the characteristic blue ;amyloid' colour with iodine in the presence of sodium sulphate, and it is suggested that this arises by the interaction of iodine molecules and possibly iodide ions within the interstices between aggregated xyloglucan chains. ;Soluble' xyloglucan is metabolized during germination and is presumed to have a reserve function. ;Insoluble' xyloglucan is metabolized less completely over the period studied but its lack of turnover during cell-wall differentiation indicates that it also is a reserve. These and other beta-(1-->4)-linked reserve polysaccharides of seeds might also have a structural function which is of particular value for the survival of the dormant seed.


Photoactivation of the manganese catalyst of O 2  evolution. I. Biochemical and kinetic aspects.


Photoactivation of the manganese catalyst of 0 2 evolution II. A two-guantum mechanism.


The professional adjustment and growth of the new graduate.


A study of registered nurses who viewed the PANMED television program series in nursing.


Cells mediating specific in vitro cytotoxicity. I. Detection of receptor-bearing lymphocytes. Spleen cells from mice immunized with allogeneic tumor cells are incubated on different fibroblast monolayers. The nonadsorbed cells are tested for cytotoxicity against (51)Cr-labeled target cells. The cytotoxicity of nonadsorbed cells is much lower after incubation on fibroblasts syngeneic to the immunizing tumor cells than after incubation on fibroblasts syngeneic to the immune cells. This specific decrease of cytotoxic activity depends on the duration and temperature of incubation on monolayers. After incubation the monolayers are trypsinized and pure populations of adsorbed lymphocytes isolated by density gradient fractionation. The cytotoxicity of such trypsin-eluted, gradient-purified lymphocytes is much higher when these lymphocytes are isolated from fibroblasts syngeneic to the immunizing tumor cells than when they are isolated from fibroblasts syngeneic to the immune cells. These experiments demonstrate specific adsorption of immune cells onto fibroblasts carrying the immunizing antigens, and thus prove the existence of specific receptors at the surface of these immune cells. Spleen cells from mice immunized with two types of allogeneic tumor cells bearing different H-2 antigen alleles are incubated on different fibroblast monolayers. The results of such experiments show a differential specific adsorption pattern, suggesting independent adsorption of two populations of immune cells bearing receptors directed against either one or the other immunizing H-2 antigen. The existence of at least a majority of cells, each of which is homogeneous as to the specificity of its receptors, makes it likely that specific receptors are synthetized by the cells that bear them. The role of specific receptor-bearing cells in the killing process is discussed.


Distribution of Ag(x) and Ag(y) antigens in the Ainu.


The action of cortisone acetate on cell-mediated immunity to infection. Suppression of host cell proliferation and alteration of cellular composition of infective foci. Pulse labeling with tritiated thymidine shows that the response in the mouse to infection with L. monocytogenes includes a large increase in the division of lymphoid cells in the spleen, an increase in the division of macrophages in the liver, and an accumulation of monocyte-derived macrophages at infective foci in the tissues. A single 2.5 mg dose of cortisone acetate given at the beginning of infection greatly delays and suppresses these three components of the host response. The unrestricted bacterial multiplication which follows cortisone treatment is ultimately because of a failure of monocyte-derived macrophages to accumulate at infective foci where they normally express immunity. The accumulation of polymorphs at these sites, in contrast, is enhanced. It is argued that cortisone acetate prevents the accumulation of monocytes at infective foci indirectly by suppressing the production in the spleen of immunologically-committed lymphocytes which are needed to mediate the cellular events at infective foci.


Cellular response to endodontic endosseous implants.


Antigen-antibody response and the elimination of Rh incompatability.


A Nursing Coordinating Council for Continuing Education.


Team teaching in community hospitals.

Team teaching in community hospitals.


Perineal care: a subject for inservice education.


Interaction of environmental factors and systemic arterial blood pressure: a review.


Let's talk it over.

Let's talk it over.


Leadership action.

Leadership action.


Norrie's disease.

Norrie's disease.


Alterations in tremor regulation after intracaudate injections of calcium ions or disodium edetate.


Do specific biochemical correlates to learning processes exist in brain cells?


An approach to ICU nurse education in a small, rural, community hospital.


Synthesis of naturally occurring polypeptides, problems of current research.


Bifunctional reagents for the crosslinking of proteins.


New method of polypeptide synthesis.

New method of polypeptide synthesis.


Financial support for continuing education.


Treatment of bronchial asthma with disodium cromoglycate (Intal) in children.


Macromolecule synthesis leading to cell division in Tetrahymena pyriformis after replacement of required amino acids.


Phenol oxidase activity in the larva of the blowfly Calliphora erythrocephala.


Fluorescent derivatives of 3-hydroxy-L-kynurenine in the lens of man, the baboon and the grey squirrel.


Sporulation in Bacillus subtilis. The appearance of sulpholactic acid as a marker event for sporulation. 1. The synthesis of sulpholactic acid in sporulating cultures of Bacillus subtilis was studied. 2. Sulpholactic acid was first detected about 4h after the initiation of sporulation and 1h before refractility. The rate of synthesis paralleled that of the other events of sporulation examined. 3. Sulpholactic acid accounted for 1.7% of the material of the spore. 4. Because the addition of chloramphenicol in the earlier stages of sporulation inhibited formation of the compound, it is likely that the enzymes concerned are synthesized de novo during sporulation. 5. In asporogenous mutants only those blocked at a late stage and showing partial refractility were able to produce sulpholactic acid. This correlation makes sulpholactic acid a useful marker event in sporulation.


The interaction of concanavalin A with teichoic acids and bacterial walls.


Biosynthesis of bacterial menaquinones (vitamins K 2 ).


Reversible inactivation of the potential pepsin activity of pepsinogen by alcohols.


Solute binding and transformation during transport.


Quantitation of plasma proteins in low concentrations using RID.


Evaluation of a new ultrasonic fetal heart motion detector.


Metiapine: a double-blind evaluation in chronic schizophrenic patients.


Loxapine succinate in the treatment of uncontrollable destructive behavior.


A pilot study of AL-1612 in chronic schizophrenics.


Electrophysiological study of the action of a new benzodiazepine derivative (ORF-8063) on the central nervous system.


Gonorrhea in women: treatment with sulfamethoxazole and trimethoprim.


Neurotropism of measles virus variants in hamsters.


Identification of an epidemic strain of group C Neisseria meningitidis by bactericidal serotyping.


The pseudomallei group: a review.

The pseudomallei group: a review.


The management of diabetic coma.

The management of diabetic coma.


An autopsy case of systemic primary amyloidosis associated with  -M pyroglobulinemia.


Tests for lead poisoning.

Tests for lead poisoning.


Plasma lipids in human linoleic acid deficiency.


Fat-induced lipaemia in rats.

Fat-induced lipaemia in rats.


Acceptance and palatability of foods by protein-deprived monkeys.


A review and evaluation of tuberculin testing procedures used for macaca species.


Comparison between the method of Liley and of Whitfield in research work on the amniotic fluid in Rh incompatibility.


Future trends in antepartum assessment of the Rh problem.


The molecular integrity of chloroplast ribosomal ribonucleic acid. Instability of chloroplast rRNA has been observed with essentially all chloroplast RNA preparations. This paper describes experiments that show that, under normal conditions of preparation and fractionation, only the heavy chloroplast component (mol.wt. 1.1x10(6)) is unstable, the light chloroplast rRNA (mol.wt. 0.56x10(6)) and the cytoplasmic rRNA species (mol.wt. 1.3x10(6) and 0.70x10(6)) being stable. The stability of the 1.1x10(6)-mol. wt. molecule varies with different plant species, as also does the size and the number of fragments produced. Cleavages in three particular regions of the molecule are very frequent within the range of tissues studied. The 1.1x10(6)-mol.wt. rRNA is, however, stabilized by the presence of Mg(2+) during the preparation and fractionation of the RNA.


The results of studying a few chemical parameters and the enzymes of amniotic fluid in Rhesus-allo-immunization.


Study of anti-Rh antibodies in the amniotic fluid.


Some effects of prostaglandins E 2  and F 2   on the pregnant rhesus monkey.


Sporulation in Bacillus subtilis 168. Comparison of alkaline phosphatase from sporulating and vegetative cells. 1. The purification of the ;vegetative' alkaline phosphatase of Bacillus subtilis 168 was simplified by ionic elution of the enzyme from intact cells. 2. The enzyme has a molecular weight of about 70000 and treatment of the enzyme with 10mm-hydrochloric acid or 6.0m-guanidine hydrochloride, beta-mercaptoethanol (0.1m) gives rise to enzymically inactive subunits. 3. The amino acid composition of the enzyme was determined. The N-terminal residue determined by the DNS chloride method is glycine. 4. The properties of this enzyme were compared with the ;sporulation' alkaline phosphatase of the same strain. 5. Although the ;sporulation' enzyme differs from the ;vegetative' enzyme in its physiology of appearance and apparent mRNA stability, an examination of properties of the enzymes revealed no differences. 6. The enzyme from both cell forms is bound to the particulate fraction of cell extracts, but can be solubilized by high concentrations of magnesium chloride; removal of the magnesium chloride, by dialysis, results in precipitation of both enzymes. Both enzymes can be removed from intact cells by ionic elution. 7. The ;vegetative' and ;sporulation' enzymes have identical pH optima, K(m) and K(i) values and electrophoretic mobilities in cellulose acetate. 8. Their half-life is 28min at 65 degrees C and their Q(10) is 1.25. 9. The molecular size determined by gel filtration on Sephadex G-100 is about 69000. 10. ;Vegetative' and ;sporulation' forms gave precipitin lines that were continuous and non-spurred when tested against antiserum prepared against the ;vegetative' enzyme. 11. The ;sporulation' alkaline phosphatase appears to be associated with stage II of sporulation and appears to be induced by something specifically concerned in sporulation and not by phosphate starvation.


Antibody titres in blood and amniotic fluid.


The source of H2O2 for the uterine fluid-mediated sperm-inhibitory system.


Errors in interpretation of amniocentesis. Results in the estimation of severity of foetal haemolytic disease.


Functional identity of anaphylatoxin preparations obtained from different species and by different activation procedures. II. Immunological identity.


Radioimmunoassay of human chorionic gonadotropin and human placental lactogen in Rh immunized pregnancies.


The chromosome of Bacillus subtilis. II. A general solution for the age distribution function of bacterial chromosomes.


Genetic marker systems in Arctic populations. I. Lp and Ag datas on the Greenland Eskimos.


The value of different methods of amniotic fluid analysis in the diagnosis of Rh hemolytic disease (spectrophotometric and chemical determination of bilirubin, bilirubin-protein ratio, anti-Rho(D) titres).


The effect of cycloheximide on membrane transport in Euglena. A comparative study with nigericin.


Structural properties of bovine brain S-100 protein.


The role of sulfhydryl groups in serological properties of bovine brain S-100 protein.


Purification and characterization of colicin E1.


Current therapy of gonorrhea.

Current therapy of gonorrhea.


National registry of long-term dialysis patients.


Effect of growth conditions on the fatty acid composition of Listeria monocytogenes and comparison with the fatty acids of Erysipelothrix and Corynebacterium.


Follow-up clinic. 9. The misfortunes of Mrs. Briggs.


Oral hypoglycaemic drugs.

Oral hypoglycaemic drugs.


Renal nosematosis in young rabbits.

Renal nosematosis in young rabbits.


Mast-cell neoplasms of the domestic cat.


Quantitative profile scanning for the measurement of organ radioactivity.


Gastric acid response to sham feeding in duodenal ulcer patients before and after antrectomy.


Pulmonary transcapillary exchange of  24 Na and  51 CrEDTA. An evaluation of factors influencing the extraction of these tracers during one passage through an isolated lung preparation.


Experimental subarachnoid hemorrhage: a study for spasm with the production of aneurysms.


Transorbital approach to the middle cerebral artery of the squirrel monkey: a technique for experimental cerebral infarction applicable to ultrastructural studies.


Ultrastructural studies of the alveolar-capillary barrier in isolated plasma-perfused rabbit lungs. Effects of EDTA and of increased capillary pressure.


The effect of electrocautery, atmospheric exposure, and surgical retraction on the permeability of the blood-brain-barrier.


Renovascular hypertension due to neurofibromatosis. Report of a case.


Roentgenologic manifestations of primary macroglobulinemia (Waldenström).


Studies on virus particles resembling oncogenic RNA viruses in monkey breast carcinoma.


Virus-like particles in human milk.

Virus-like particles in human milk.


Status of adjuvant therapy: results of The National Surgical Adjuvant Breast Project studies on oophorectomy, postoperative radiation therapy, and chemotherapy. Other comments concerning clinical trials.


The mediator of cellular immunity. IV. Cooperation between lymphocytes and mononuclear phagocytes.


Right aortic arch.

Right aortic arch.


Noradrenergic mediation of experimental cerebrovascular spasm.


Sperm antibodies as a cause of infertility.


Australia antigen and hepatitis: a comprehensive review.


Nuclear morphology in the nutritive cells of Drosophila testes.


Nifurpipone, a new nitrofuran with a large antimicrobial spectrum.


Environmental modification for the older person.


Tolerances for cerebral concussion from head impact and whiplash in primates.


Clinicopathological study of leucorrhoea.


Nature and regulation of energy metabolism in the epidermis.


Autoimmunity to human sweat. I. Demonstration of antigens in sweat reactive with autologous and homologous sera.


Threshold levels for damage of the cornea following irradiation by a continuous wave carbon dioxide (10.6 millimicron) laser.


Effect in the rhesus monkey of salicylate on centrally-induced endotoxin fevers.


Long-term treatment of parkinsonism with levodopa.


Lead intoxication in children. Diagnosis and treatment.


Antigen binding cells in tolerance and immunity.


Penetration of some microorganisms in meat.


The enzymic formation of O-acetylhomoserine in Bacillus subtilis and its regulation by methionine and S-adenosylmethionine.


The effect of ferric ion on phycocyanin fluorescence.


Organization of cerebral cortex for perception.


The community medicine of cerebrovascular disease.


Opacity pulse propagation in internal and external carotid vascular beds of experimental animals.


Temporary occlusion of the middle cerebral artery in the monkey: clinical and pathological observations.


The management of lymphoma.

The management of lymphoma.


The distribution of cell generation times.


Effects of mammalian gonadotropins on ovary in the toad Bufo bufo bufo (L.).


Regulation of ovarian cycle in the toad Bufo bufo bufo (L.): effects of autografting pars distalis of the hypophysis, of extirpating gonadotropic hypothalamic region, and of partial ovariectomy.


Evolutionary biology of prolactins and somatotropins. II. Electrophoretic comparison of tetrapod somatotropins.


Unproven methods of cancer management.

Unproven methods of cancer management.


The pathology of tumors. 4. Grading, staging and classification of neoplasms.


Effect of aldosterone and oxytocin on the active sodium transport across the isolated toad skin in relation to loosening of stratum corneum.


Is the toad pars intermedia under both excitatory and inhibitory nervous control?


William Ernest Miles (1869-1947).

William Ernest Miles (1869-1947).


Prolactin, calcitonin, and blood calcium in the toads Bufo boreas and Bufo marinus.


Gangliosides in human skin fibroblasts and their enrichment in the "Hurler variant" and Krabbe's disease.


A method of performing abdomino-perineal excision for carcinoma of the rectum and of the terminal portion of the pelvic colon (1908).


The effects of thyrocalcitonin on ionic and total plasma calcium in monkeys.


Macromolecular synthesis in Bacillus subtilis during development of the competent state. Rates of deoxyribonucleic acid, ribonucleic acid, and protein synthesis were examined in purified competent cells of Bacillus subtilis during the development of the transformable state. To become competent, a cell must depart from the normal course of vegetative growth and pass through a precompetent phase beginning as early as 90 to 180 min before the appearance of transformability. While in the precompetent state, the cell decreases its rate of deoxyribonucleic acid synthesis and lowers its ratio of ribonucleic acid synthesis to protein synthesis. This altered pattern of synthesis eventually leads to a decreased buoyant density of precompetent cells. Once a cell has become both precompetent and low in density, it can be converted to a competent (transformable) cell. The early alterations in macromolecular synthesis were found in two competence regimens, one utilizing a nutritional step-down and one free of such a shift. The data imply that the precompetent state is a generalized characteristic of the B. subtilis transformation system and is not specific to the procedure used to allow competence development. Since precompetence-specific events occur very early in a competence regimen, we conclude that the induction of precompetence is unrelated to sporulation or a nutritional shift.


Fate of transforming deoxyribonucleate in Bacillus subtilis. The majority of donor deoxyribonucleate (DNA) at early stages after uptake was found in a complex with a cell component which changes its buoyant behavior on equilibrium density gradients. Analysis of the recipient cell lysates, after treatment to dissociate the complex, showed about two-thirds of the donor molecules in denatured form and the rest associated with recipient DNA. Incubation of cells after DNA uptake leads to the disappearance of denatured donor DNA and to the increase of donor label associated with recipient DNA. Some characteristics of a component from intact cells or spheroplasts with affinity for denatured Bacillus subtilis DNA are described.


Total synthesis of acetate from CO 2 : methyltetrahydrofolate, an intermediate, and a procedure for separation of the folates. Whole-cell preparations of Clostridium thermoaceticum were exposed to a short pulse of (14)CO(2) under conditions in which double-labeled acetate was synthesized. Radioactive methyltetrahydrofolate monoglutamate, diglutamate, and triglutamates were isolated from extracts of the cells. The radioactivity was found to be exclusively in the five methyl position. The specific activities of the methyltetrahydrofolate derivatives were very high and were in accord with the proposal that methyltetrahydrofolates are the precursors of the methyl of acetate. A new method of separation of folates employing QAE-Sephadex chromatography and a linear gradient with triethylammonium bicarbonate is presented which completely resolves the common folate monoglutamates and, upon freeze-drying, yields salt-free preparations.


Metabolism of poly- -hydroxybutyrate: effect of mild alkaline extraction on native poly- -hydroxybutyrate granules. Mild alkaline extraction of native poly-beta-hydroxybutyrate (PHB) granules results in the solubilization of a protein fraction. Both the solubilized protein fraction and the extracted granules are essentially devoid of PHB synthetase activity unless recombined. The protein fraction has been separated by chromatography into two components (A-I and A-II). A-I but not A-II can be recombined with extracted granules to give rise to PHB synthetase activity. Extracted granules no longer require pretreatment with activator or trypsin but are directly susceptible to hydrolysis by Rhodospirillum rubrum depolymerase. Addition of A-II or A-I prevents the direct hydrolysis by depolymerase. The inhibition is reversed by activator or trypsin. We conclude that native granules are associated with a protein inhibitor which prevents the hydrolysis of PHB by depolymerase unless the protein is destroyed by trypsin, removed by alkaline extraction, or modified by activator.


-aminobutyric acid as a required germinant for mutant spores of Bacillus megaterium. Germinated spores of Bacillus megaterium QM B1551 were irradiated with ultraviolet light, and spore-forming survivors were screened for germination requirements. Spore strains which failed to germinate in a variety of defined solutions germinative for spores of the parent strain were obtained. Mutant spores germinated readily in solutions containing yeast extract or one of numerous complex preparations. gamma-Aminobutyric acid, obtained from yeast extract by column chromatography, was shown to be required for germination by the mutant spores. gamma-Aminobutyric acid and l-alanine at final concentrations of 1 mm each, in solutions of KI (40 mm), equaled the potency of yeast extract (1 mg/ml) in the germination of the mutant spores. One of several other amino acids could be substituted, though less effectively, for l-alanine. alpha-Aminobutyric acid, beta-aminobutyric acid, beta-alanine, and 5-aminovaleric acid were ineffective substitutes for gamma-aminobutyric acid in mutant spore germination.


Macromolecular sieving by the dormant spore of Bacillus cereus. The threshold surface porosity in the dormant spore of Bacillus cereus strain T was assessed by measuring passive permeabilities to a series of polydisperse polyethylene glycol samples which increased in average molecular size. The apparent exclusion threshold at diffusional equilibrium corresponded to a polymer of number-average molecular weight ( M(n)) = 150,000 and equivalent hydrodynamic radius ( r(ES)) = 16 nm, which confirmed a previous report. However, analytical gel chromatography before and after uptake by the spores revealed that only the low molecular weight fractions in a polymer sample distribution were taken up. From graphical analyses of the changes in molecular weight distributions, a quasi-monodisperse exclusion threshold was determined corresponding to M(n) = 8,000 and r(ES) = 3.2 nm. Thus, the equivalent porosity in the limiting outer integument appeared much more restrictive than heretofore shown for spores, although still more open than the monodisperse equivalent for the cell wall of vegetative bacilli.


Amino acid- -naphthylamide hydrolysis by Pseudomonas aeruginosa arylamidase. The intracellular and constitutive arylamidase from Pseudomonas aeruginosa was purified 528-fold by salt fractionation, ion-exchange chromatography, gel filtration, and adsorption chromatography. This enzyme hydrolyzed basic and neutral N-terminal amino acid residues from amino-beta-naphthylamides, dipeptide-beta-naphthylamides, and a variety of polypeptides. Only those substrates having an l-amino acid with an unsubstituted alpha-amino group as the N-terminal residue were susceptible to enzymatic hydrolysis. The molecular weight was estimated to be 71,000 daltons. The lowest K(m) values were associated with substrates having neutral or basic amino acid residues with large side chains with no substitution or branching on the beta carbon atom.


Chemical characterization of a new surface antigenic polysaccharide from a mutant of Staphylococcus aureus. A mutant of Staphylococcus aureus H was isolated by virtue of its inability to agglutinate with antibodies against teichoic acid of S. aureus. Immunological studies revealed that the mutant, S. aureus T, possessed a new surface antigen in addition to having the antigenic determinant of the wild-type strain, the ribitol teichoic acid. The presence of this additional surface component rendered strain T resistant to staphylococcal typing phages, presumably by masking the phage-receptor sites. The polymer was separated from teichoic acid by chromatography on diethylaminoethyl cellulose and was shown to be composed of two amino sugars, N-acetyl-d-fucosamine and N-acetyl-d-mannosamin uronic acid.


Studies on the division cycle of mammalian cells. V. Modifications of time parameters by different steady-state culture conditions. In cultures of murine neoplastic mast cells, the duration of different phases of the division cycle (G(1), S, G(2), and mitosis [M]) was determined under optimal and several well-defined suboptimal growth conditions. Two methods of evaluation were applied to the same culture system: first, the relative number of G(1), S, G(2), and M cells was determined by pulse labeling of samples with thymidine-(3)H and subsequent radioautography in conjunction with a microfluorometric technique permitting rapid measurements of cellular DNA content; second, after pulse labeling with thymidine-(3)H, the variations with time of the mitotic labeling index were analyzed. Suboptimal culture conditions were obtained by reducing the concentration of single essential medium components (leucine, glucose, or serum) or by the addition of specific metabolic inhibitors (actinomycin D, amethopterin). Growth-limiting culture conditions resulted in increased generation times. Even under control conditions, the cell number doubling time exceeded the generation time, and this difference was more pronounced in suboptimal media. Under most of the suboptimal conditions tested, the increase in generation time was attributable primarily to an extended duration of the G(1) phase. Under certain growth-limiting conditions, however, other phases were also prolonged. In addition, the variabilities of the generation time and of certain cell cycle phases were increased under suboptimal culture conditions. Results obtained by the two methods of evaluation were, in general, in good agreement with each other. Some differences were, however, observed and interpreted in terms of cell death and/or asymmetric frequency distributions of cell cycle parameters.


Labeling of murine mastocytoma cells in vitro with plasma tritiated thymidine-labeled animals. 40 min after injecting tritiated thymidine into an animal, 20-30% of the total plasma radioactivity is nonvolatile. This fraction decreases to about 6% 10 hr after the injection and 3% 24 hr after the injection. There appears to be material in this nonvolatile fraction that can label mastocytoma cells in culture. The labeling indices decrease with time after injection in the same way as the nonvolatile fraction. The 40 min plasma sample contains sufficient material to allow accurate assessment of the fraction of cells in S in culture after a 6 wk exposure. The circulating material is not apparently available for incorporation into those cells in cycle in the donor animal. The material appears to be related to the G(0) cell-specific pool that has been described elsewhere. The trichloroacetic acid-soluble or ethanol-soluble nonvolatile activity appears to contain thymine, and some thymidine-phosphorylated compounds.


Gargoylism: Hurler's syndrome, a case history.


Determination of lactate in parenterals containing reducing sugars.


Pseudomonas aeruginosa contamination of liquid antacids: a survey.


Hybridization of guinea pig anti-ferritin and anti-rabbit intestinal sucrase.


Hepatitis associated antigen in acute viral hepatitis in Greece.


Subtypes in human immunoglobulin lambda-chains.


Disodium cromoglycate (Lomudal) in asthma with emphasis on lung function tests.


Effect of injecting testicular suspension and spermia on spermatogenesis in guinea pigs.


Venous phase in spinal cord angiography.


Alfalfa mosaic virus.

Alfalfa mosaic virus.


Elation.

Elation.


The catabolism of phosphatidylethanolamine by the rumen protozoon Entodinium caudatum and its conversion into the N-(1-carboxyethyl) derivative. 1. The N-(2-hydroxyethyl)alanine esterified to phosphatidic acid in anaerobic ciliate rumen protozoa has the l configuration. 2. Labelling experiments with Entodinium caudatum cultures using [(32)P]P(i) [2-(14)C]ethanolamine and (32)P- and (14)C-labelled phosphatidylethanolamine show that phosphatidylethanolamine is the direct lipid precursor of the N-(2-hydroxyethyl)alanine-containing phospholipid. 3. Labelling experiments with [(14)C]starch, [(14)C]lactate and [(14)C]pyruvate with E. caudatum cultures indicate that a three-carbon glycolytic intermediate is probably the precursor of the N-(1-carboxyethyl) grouping which substitutes on the amino group of phosphatidylethanolamine. 4. [(32)P]phosphatidylethanolamine is catabolized by E. caudatum forming initially glycerylphosphorylethanolamine and subsequently glycerophosphate and P(i). A little phosphorylethanolamine formed may possibly arise from bacterial enzymes ingested by the protozoa.


Cross-linking of DNA by alkylating agents and effects on DNA function in the chick embryo.


Role of body temperature in the definition of retinal burn threshold.


Symptomatic diverticula. Complications of duodenal diverticula: a case report of obstruction.


Effects of a prostaglandin antagonist, polyphloretin phosphate (PPP), on basal and stimulated thyroid function.


Stimulation of erythrocyte glycolysis by fear and epinephrine injection.


Biological disposition and metabolic fate of fluphenazine- 14 C in the dog and rhesus monkey.


Enzymes in the urine.

Enzymes in the urine.


Kinetics of potassium distribution in man using isotope dilution and whole-body counting.


Immunoassay for human calcitonin. II. Clinical studies.


Effect of treatment on calcium kinetics in metabolic bone disease.


Antileukaemic and nephrotoxic properties of platinum compounds.


Multiplication of the scrapie agent.

Multiplication of the scrapie agent.


Extranuclear DNA and the endosymbionts of Paramecium aurelia.


Labyrinthine concussion.

Labyrinthine concussion.


Opsonic defect in patients with cystic fibrosis of the pancreas. Cystic fibrosis of the pancreas is one of the most common inborn errors of metabolism. The high incidence of morbidity and mortality in these patients is primarily due to severe and frequent pulmonary infection. To date, no immune deficiency has been found in cystic fibrosis patients. Their sera contain normal quantities of immunoglobulins and hemolytic complement. In an assay of phagocytosis by alveolar macrophage, six out of nine sera from cystic fibrosis patients failed to support normal phagocytosis of Pseudomonas aeruginosa. This deficiency could be corrected by increasing the concentration of serum used in the assay. By contrast, their sera supported normal phagocytosis of Pseudomonas by blood polymorphonuclear leukocytes and continued to support normal phagocytosis when serum dilutions were used. Two patients with severe isolated deficiences of serum immunoglobulin A were found to have a similar defect in the alveolar macrophage assay, but normal phagocytosis by polymorphonuclear leukocytes. It is postulated that cystic fibrosis patients may have a quantitative and (or) functional defect of IgA antibodies, specific for Pseudomonas, and possibly of importance in the pathogenesis of their pulmonary disease.


Isolation of a 5S RNA-protein complex from mammalian ribosomes. 5S RNA is removed from the large ribosomal subunit of both rat liver and rabbit reticulocyte ribosomes by treatment with EDTA to remove magnesium ions. The RNA is removed not as a naked molecule, but in association with a single ribosomal protein (molecular weight about 35,000) as a ribonucleoprotein, sedimenting at about 7 S. Conditions for the exclusive removal of the 5S RNA-protein complex have been established. Both ribosomal subunits undergo distinct changes in their sedimentation rate, concomitant with the loss of their biological activity, when sedimented into sucrose gradients containing high concentrations of monovalent ions and low concentrations of magnesium. Under these conditions the large subunit loses 5S RNA, the 5S RNA-associated protein, and several more proteins.


Esophageal intramural diverticulosis: a re-evaluation.


Immunoconglutinin and complement studies in congenital nephrotic syndrome and nephritis of Henoch-Schönlein purpura in children.


Relationship between phosphates and alkaline phosphatase of Anabaena flos-aquae in continuous culture.


Intravenous feeding in infants.

Intravenous feeding in infants.


Vestibular single cell recording in the awake monkey.


Separation and partial characterization of some human erythrocyte membrane protein components.


Factors affecting the interactions of proteins isolated from erythrocyte membranes.


Enzymic explorations of the structures of starch and glycogen.


Treatment of obsessional illnesses and phobic anxiety states with clomipramine.


The bactoprenol content of plasma and mesosome membranes from Lactobacillus casei.


Electrophoretic and immunological characterization of rat neurophysin. 1. The electrophoretic properties of rat posterior pituitary proteins have been compared on starch gel with those of bovine and porcine neurophysins. 2. [(35)S]-Cysteine was injected into the supraoptic nucleus of male rats and 16-24h later the distribution of labelled neural-lobe protein in starch and polyacrylamide gels was determined. In both systems a single major protein component was found to contain more than 80% of the total recovered radioactivity. Between 5 and 10% of the radioactivity was found in a minor component in polyacrylamide gel. 3. In agar, microimmuno-diffusion and -electrophoresis of the rat neural-lobe proteins gave a single arc with neurophysin antiserum, and after starch-gel electrophoresis this arc was shown to be due to the major labelled component. 4. The molecular weights of the rat neural-lobe proteins were estimated by polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate. The molecular weight of the major labelled component was found to be 12000. 5. It is concluded that the rat neurophysin consists of one major and possibly one minor component.


Serologic, biochemical, and cultural traits of enterotoxigenic and coagulase-positive staphylococci isolated from food handlers.


Using one dose of doxycycline or penicillin to treat men with gonococcal urethritis.


Studies on antibiotic syngerism against enterococci. II. Effect of various antibiotics on the uptake of  14 C-labeled streptomycin by enterococci. The mechanism by which agents that inhibit bacterial cell wall synthesis produce a synergistic effect against enterococci when combined with aminoglycoside antibiotics has not been elucidated. Using (14)C-labeled streptomycin, it could be shown that uptake of this aminoglycoside antibiotic was markedly enhanced in enterococci growing in the presence of penicillin or other agents which inhibit the synthesis of bacterial cell walls. There was no enhancement of streptomycin uptake when the cells were incubated with antibiotics which primarily affect the bacterial cell membrane or inhibit protein synthesis. Increased streptomycin uptake was produced by penicillin only in actively growing bacteria. These observations are consistent with the hypothesis that enterococci exhibit a natural barrier to the entry of streptomycin which can be overcome by agents which inhibit cell wall synthesis, thus producing a synergistic effect.