Studies of the secretion of monkey placental lactogen. A radioimmunoassay for monkey placental lactogen (MPL) was developed to study the factors controlling the secretion of MPL. The sensitivity of the assay was 1 ng MPL per ml. Human and monkey growth hormone, and human placental lactogen (HPL) showed minimal cross-reactions with MPL. Maternal MPL concentrations as measured in 40 rhesus monkeys increased progressively throughout pregnancy to a mean of 5000 ng/ml at term while umbilical vein MPL was less than 50 ng/ml. After term delivery maternal MPL concentrations decreased rapidly with a t(1/2) of 20 min.After fetectomy but with retention of the placenta, MPL concentrations decreased by 25% reaching a plateau over a 6 hr period. Experimental abruption of the secondary placenta within 1 hr produced a 50% decrease in MPL concentration. After ligation of the fetal vessels supplying the secondary placental disc, MPL increased transiently and then decreased to levels significantly below those of the control period. These studies suggest MPL secretion is not directly controlled by the fetus but is sensitive to changes in placental blood flow. The pregnant rhesus monkey serves as a useful model for investigating factors which may regulate HPL secretion because of the close similarity between MPL and HPL secretion.


Serum triiodothyronine: measurements in human serum by radioimmunoassay with corroboration by gas-liquid chromatography. Serum triiodothyronine (T(3)) has been measured by radioimmunoassay and corroborated by analysis of the identical samples with a previously described gas-liquid chromatographic technique. Special features of the radioimmunoassay procedure which permit determinations in unextracted serum include the use of a T(3)-free serum preparation for the construction of the standard curve and of tetrachlorothyronine to inhibit binding of T(3) to thyroxine-binding globulin.T(3) values by radioimmunoassay were 138 +/-23 ng/100 ml (mean +/-SD) in 82 normal subjects, 62 +/-9 ng/100 ml in 45 hypothyroid patients, and 494 +/-265 ng/100 ml in 60 patients with toxic diffuse goiter. In the hypothyroid group, the range was similar in patients with both primary and secondary hypothyroidism. There was no overlap between the three thyroidal states. Elevated T(3) levels were seen in 40 cases that appeared clinically hyperthyroid but had normal serum thyroxine (T(3)) determinations, a syndrome we have called T(3) toxicosis. Values obtained with radioimmunoassay agreed closely with those we had previously found by gas-liquid chromatography which were 68 +/-2 ng/100 ml in hypothyroidism, 137 +/-23 ng/100 ml in normal subjects, and 510 +/-131 ng/100 ml in untreated toxic diffuse goiter. Since T(3) is very potent and its level varies in different clinical states, accurate T(3) measurements are required to assess a patient's thyroid status properly. The radioimmunoassay for T(3) appears to be sufficiently sensitive, precise, and simple to permit its routine clinical application for this purpose.


Effects of amines on the level of N-acetyl-aspartate and N-acetyl-aspartyl-glutamate in mouse brain tissue slices.


Solid-state chemistry of cromolyn sodium (disodium cromoglycate).


Removal of ferrous ions by N,N'-ethylenediaminetetraacetic acid in microbiological assay of pyridoxine.


Drug transfer across rat intestinal musculature after edetic acid treatment.


Isolation of nucleic acids from microfungi by treatment with thioglycolic acid.


Empyema.

Empyema.


The oral manifestations of cyclopia.

The oral manifestations of cyclopia.


Return to the land of the living: an approach to the problem of chronic hemodialysis.


Systemic bone disease developing in small premature infants.


Biochemical aspects of intravenous alimentation.


Absent IgA associated with normal chromosomes.


Pseudomonas agglutinins in patients with cystic fibrosis.


Diabetic ketosis and coma.

Diabetic ketosis and coma.


Radiation-induced heart disease. Clinical and experimental aspects.


Symptomatic anorectal gonorrhea in an adolescent female.


Immunology as a laboratory tool.

Immunology as a laboratory tool.


Pharmacologic effects of nicotine upon the fetus and mother in the rhesus monkey.


Denaturation of globular proteins by guanidine thiocyanate. I. Optical rotation in aqueous guanidine thiocyanate solutions.


Studies on proteins of animal ribosomes. 8. Two-dimensional polyacrylamide gel electrophoresis of ribosomal proteins of rat liver.


Effect of oxidation on the transition of penicillinase conformation.


Antibiotic resistant gonococci.

Antibiotic resistant gonococci.


Education of diabetic patients.

Education of diabetic patients.


Some biochemical properties of isolated Bufo arenarum liver mitochondria.


Immunoglobulin levels in Hodgkin's disease.


Immunochemical and physicochemical properties of haptoglobin, antihemoglobin antibody, and their complexes with hemoglobin.


Interaction of anti-DNP rabbit antibody with peptid Fab' fragment of homologous IgG.


Loss of macrophages from peritoneal exudates of sensitized rats after injection of streptococcal antigens.


Studies on IgE fragments prepared by papain digestion.


Applied optical principles.

Applied optical principles.


The use of IgE mediated responses as a pharmacologic test system. The effect of disodium cromoglycate in respiratory and cutaneous reactions and on the electrocardiograms of rhesus monkeys.


Solubility of membrane proteins in aqueous media.


Regulation of rat liver glycogen synthetase. Relationship of the hormonal activation and the time-dependent in vitro activation.


Two aspartate transport systems in Escherichia coli.


Feline leukemia virus: purification from tissue culture fluids.


Peripheral nerve lesions similar to those of Marek's disease in chickens inoculated with reticuloendotheliosis virus.


Methods for the detection of viral antigen and antibody to a feline leukemia virus (a preliminary report).


Effect of actinobolin on nucleic acid and protein synthesis in Streptococcus faecalis.


Occurrence of lysophosphatides in bacteriophage T4rII-infected Escherichia coli S-6. Hydrolysis of phospholipids was observed to start about 15 min after Escherichia coli S/6 cells were infected with T4rII bacteriophage mutants. Hydrolysis continued through the latent period and well past the time when cell lysis occurs. The hydrolytic products that accumulated were free fatty acids, 2-acyl lysophosphatidylethanolamine, and various lysocardiolipins. These products indicated the action of phospholipase A(1). From 15 to 22 min after infection, there were equivalent amounts of fatty acids and lysophosphatides in extracts of cellular lipids. Thereafter, free fatty acids were produced in excess. This suggests that lysophospholipase was active at the later time. We also observed a stoichiometric relation between loss of phosphatidylglycerol and increase of cardiolipin plus lysocardiolipins. This continued well past the normal lysis time (25 min). The appearance of lipase activities during the latent period seems to be specific to infection with rII mutants. Neither the wild-type bacteriophage nor rI mutants produced similar activities by 22 min after infection.


Heat induction of prophage phi 105 in Bacillus subtilis: replication of the bacterial and bacteriophage genomes. A temperature-inducible mutant of temperate Bacillus bacteriophage phi105 was isolated and used to lysogenize a thymine-requiring strain of Bacillus subtilis 168. Synthesis of phage and bacterial deoxyribonucleic acid (DNA) was studied by sucrose gradient centrifugation and density equilibrium centrifugation of DNA extracted from induced bacteria. The distribution of DNA in the gradients was measured by differential isotope and density labeling of DNA before and after induction and by measuring the biological activity of the DNA in genetic transformation, in rescue of phage markers, and in infectivity assays. At early times after induction, but after at least one round of replication, phage DNA remains associated with high-molecular-weight DNA, whereas, later in the infection, phage DNA is associated with material of decreasing molecular weight. Genetic linkage between phage and bacterial markers can be demonstrated in replicated DNA from induced cells. Prophage induction is shown to affect replication of the bacterial chromosome. The overall rate of replication of prelabeled bacterial DNA is identical in temperature-induced lysogenics and in "mock-induced" wild-type phi105 lysogenics. The rate of replication of the bacterial marker phe-1 (and also of nia-38), located close to the prophage in direction of the terminus of the bacterial chromosome, is increased in induced cells, however, relative to other bacterial markers tested. In temperature-inducible lysogenics, where the prophage also carries a ts mutation which blocks phage DNA synthesis, replication of both phage and bacterial DNA stops after about 50% of the phage DNA has replicated once. The results of these experiments suggest that the prophage is not initially excised in induced cells, but rather it is specifically replicated in situ together with adjacent parts of the bacterial chromosome.


Early tissue response to rapid maxillary expansion in the midpalatal suture of the rhesus monkey.


Response of the rhesus monkey, Macaca mulatta, to continuously infused staphylococcal enterotoxin B.


Studies on the genetic control of tryptophan pyrrolase in Drosophila melanogaster.


Compatibility groups among fi -  R factors.


Localization of nitrogen fixation in Anabaena.


Antigenic variation in Babesia rodhaini demonstrated by immunization with irradiated parasites.


Nutrition and cancer. 1. Problems due directly to tumors and associated diseases.


Selectivity of proteinuria in nephrotic syndrome.


Preservation of sodium bicarbonate eye lotion BPC against contamination with Pseudomonas aeruginosa.


Cell aggregation and differentiation in Dictyostelium.


Studies on glucose utilization and insulin responsiveness of human subcutaneous adipose tissue in obese and non-obese subjects.


The separation of fifty pesticides and related compounds and polychlorobiphenyls into four groups by silica gel microcolumn chromatography.


Results of selection for susceptibility and resistance to trypan blue teratogenesis in a four-way outcross mouse population.


In vitro culture of Drosophila melanogaster embryonic cells.


A professional enrichment series.

A professional enrichment series.


Report from North Dakota--recognition for continuing education.


Recent advances in the cerebromacular degenerations.


Some kind of involvement.

Some kind of involvement.


Self-development.

Self-development.


Adult learning principles: the individual curriculum and nursing leadership.


The educational play.

The educational play.


Salute to the American Nurses' Association, Inc. 1896-1971.


Chromosomal distribution of rapidly reannealing DNA in Drosophila melanogaster. Cytological hybridization has been used to localize fractions of rapidly reannealing DNA in salivary chromosomes of Drosophila melanogaster. Complementary RNA of high specific activity was transcribed from hydroxyapatite-fractionated rapidly reannealing sequences and from selected buoyant-density fractions of total DNA. It was then hybridized to chromosome squashes after denaturation of DNA in NaOH. Highly "repeated" DNA sequences were detected over much of the chromosome, but were concentrated in chromocentric heterochromatin. A family of sequences with a low percentage of guanosine plus cytidine was highly concentrated in a particular region within the chromo-center. One "euchromatic" region near the tip of chromosome arm 3L also exhibited a concentration of repeated sequences.


Role of phosphorylation coupling factor in light-dependent proton translocation by Rhodopseudomonas capsulata membrane preparations. The activity of the light-dependent proton pump (in the absence of phosphorylation substrates) of Rhodopseudomonas capsulata "membrane vesicles," in contrast to that of chloroplasts, is not appreciably affected by detachment of phosphorylation coupling factor(s). Proton uptake by such "uncoupled" (low phosphorylation activity) preparations is also unaffected by the addition of phosphorylation substrates (ADP + arsenate + Mg(2+)). The H(+) pump of "coupled" preparations, however, is stimulated when all of the substrates are present simultaneously. Oligomycin appears to affect both photophosphorylation and the H(+) pump by interaction with a component of the energy transfer sequence that is distinct from coupling factor. The results obtained suggest that in photosynthetic bacteria light-dependent cyclic electron flow (the driving force for photophosphorylation) is accelerated by active phosphorylation which, in turn, is dependent on the presence of protein coupling factors in the energy-converting membrane.


Symmetric replication of the Bacillus subtilis chromosome. Synchronously replicating chromosomes in germinating spores of Bacillus subtilis were labeled near the origin with [(3)H]bromouracil. The label appeared in heavy-light DNA. When cell growth and chromosome replication were continued in unlabeled bromouracil, nearly all the tritium label was transferred to heavy-heavy DNA while the terminus remained unreplicated. This implies that both origins of the replicating chromosome can undergo reinitiation. Therefore, during multifork replication the chromosome takes on the symmetric "dichotomous" form rather than the asymmetric configuration predicted by the rolling circle model.


Temperature-sensitive mutations in Drosophila melanogaster. IV. A mutation affecting eye facet arrangement in a polarized manner. Drosophila melanogaster females heterozygous for the mutation N(60g11) have wild type eyes when raised at 29 degrees C but a disrupted arrangement of facets and extra bristles at 21 degrees C. Shifts of cultures from one temperature to the other at different stages in development revealed that facet arrangement is altered by temperature during the third larval instar. The facet pattern is affected in a vertical wave that proceeds anteriorly from the posterior rim of the eye. The role of the Notch locus in development is briefly discussed.


The relationship of African apes, man and old world monkeys. The conclusions of Wilson and Sarich (Proc. Nat. Acad. Sci. USA, 63, 1088-1093 (1969) that the human lineage diverged from that leading to the African apes about 4 to 5 million years ago is shown to be based upon a wholly unsupported assumption that the Superfamilies Hominoidea and Cercopithecoidea only separated from each other some 30 million years ago. This is entirely contrary to most recent palaeontological evidence.


Lysis of Bacillus subtilis by bacteriophage SP82 in the absence of DNA synthesis.


Chemical and immunological characterization of a basement membrane-like glycoprotein in the rat.


The metabolism of parenteral iron chelates.


A rapid accurate procedure for the determination of serotonin in whole human blood.


Absorption of oral chelated iron.

Absorption of oral chelated iron.


The sampling-boat TM  technique for determination of lead in blood and urine by atomic absorption.


Hyaluronidase and sulfatase deficiency in Hurler's syndrome.


Determination of rubidium in human erythrocytes, plasma, and urine by atomic absorption spectrophotometry.


Specificity of exercise in exercise-induced asthma. Ventilatory function after three types of exercise-running, cycling, and swimming-was studied in 10 control subjects and 40 asthmatic patients. All performed eight minutes of submaximal aerobic exercise during each of the programmes, which were conducted in a randomly selected order. Biotelemetric monitoring of heart rates was used to equate the intensity of the exertion undertaken during the three systems of exercise. No control subject showed any significant variation in ventilatory capacity after exercise, and the responses after the three forms of exercise did not differ.In asthmatics exercise-induced asthma was observed after 72.5% of running tests, 65% of cycling tests, and 35% of swimming tests. In addition, those patients who developed exercise-induced asthma after swimming were noted to have significantly smaller falls in FEV(1) levels than were recorded after running and cycling. These results were statistically significant (P <0.01).The unexplained aetiology of increased airways resistance after exercise in asthmatics is discussed. This study indicates that swimming should be recommended in preference to running or cycling as an exercise programme for adults and children with asthma.


Clinical evaluation of perhexiline maleate in patients with angina pectoris. This paper reports a double-blind trial of a new antianginal drug, perhexiline. Fifty-five patients suffering from angina pectoris were studied for periods of 12 or 24 weeks in a cross-over comparison against a placebo in four centres in the United Kingdom and Ireland. Perhexiline was effective in most patients as judged by reducing the number of anginal attacks in 84% and the consumption of glyceryl trinitrate tablets in 64%. The major side effect, dizziness, noted in one-third of the patients, may be dose/body-weight related. Perhexiline is a valuable new agent for the treatment of patients with angina, especially those who do not respond to other antianginal agents.


Detection of hepatitis associated antigen by the latex agglutination test. Hepatitis associated antigen may be detected quickly and reliably by the latex agglutination test, using antiserum from guinea pigs immunized with the antigen. The latex test has a sensitivity comparable to the counter current immunoelectrophoresis technique.


Prolonged fever in bacterial meningitis.


Studies related to penicillins. VI. The conversion of penicillin V and 6-aminopenicillanic acid into cepham derivatives.


Surgical experimentation and gross postnatal growth of the face and jaws.


Problem orientation of medical records. Advantages in long-term illness.


Hormonal and nutritional factors influencing muscle cell growth.


Herniation of the stomach presenting as a diverticulum.


Quantitative evaluation of mandibular condylar growth using tritiated thymidine in autoradiographic analysis.


Alterations in craniofacial growth resulting from unilateral fracture of the mandibular condyle in a young rhesus monkey.


Autoradiographic study of the temporomandibular articulation in the monkey.


Automated measurement of proteolytic enzymes.


Evaluation of the implant technique (in experimental studies of growth, development, and effects of force application).


Quantitative determination of total sulfur in phosphoproteins, with special reference to casein.


Dry-heat resistance of Bacillus subtilis var. niger spores on mated surfaces. Bacillus subtilis var. niger spores were placed on the surfaces of test coupons manufactured from typical spacecraft materials (stainless steel, magnesium, titanium, and aluminum). These coupons were then juxtaposed at the inoculated surfaces and subjected to test pressures of 0, 1,000, 5,000, and 10,000 psi. Tests were conducted in ambient, nitrogen, and helium atmospheres. While under the test pressure condition, the spores were exposed to 125 C for intervals of 5, 10, 20, 50, or 80 min, with survivor data being subjected to a linear regression analysis that calculated decimal reduction times. Differences in the dry-heat resistance of the test organism resulting from pressure, atmosphere, and material were observed.


Serological typing of 31 achromogenic and 40 melanogenic Pseudomonas aeruginosa strains. Thirty-one achromogenic and 40 melanogenic Pseudomonas aeruginosa strains were studied with 10 monovalent typing sera (3). Twenty-one of the achromogenic (67.7%) and seven of the melanogenic (17.5%) strains were agglutinated by one of the 10 typing sera. Ten achromogenic and 33 melanogenic strains were not agglutinated by any of the 10 typing sera. As far as this set of antisera is concerned, the typability of achromogenic and melanogenic P. aeruginosa strains appears to be much lower than that of the chromogenic, nonmelanogenic strains of the species reported previously.


Thermal inactivation of aerosolized Bacillus subtilis var. niger spores. A hot-air sterilizer capable of exposing airborne microorganisms to elevated temperatures with an almost instantaneous heating time was developed and evaluated. With this apparatus, aerosolized Bacillus subtilis var. niger spores were killed in about 0.02 sec when exposed to temperatures above 260 C. This is about 500 times faster than killing times reported by others. Extrapolation and comparison of data on the time and temperature required to klll B. subtilis var. niger spores on surfaces show that approximately the same killing time is required as is necessary for spores in air, if corrections are made for the heating time of the surface.


Metabolic factors affecting enhanced phosphorus uptake by activated sludge. Activated sludges obtained from the Rilling Road plant located at San Antonio, Tex., and from the Hyperion treatment plant located at Los Angeles, Calif., have the ability to remove all of the orthophosphate normally present in Tucson sewage within 3 hr after being added to the waste water. Phosphorus removal was independent of externally supplied sources of energy and ions, since orthophosphate and (32)P radioactivity were readily removed from tap water, glass-distilled water, and deionized water. Phosphorus uptake by Rilling sludge in the laboratory appears to be wholly biological, as it has an optimum pH range (7.7 to 9.7) and an optimum temperature range (24 to 37 C). It was inhibited by HgCl(2), iodoacetic acid, p-chloromercuribenzoic acid, NaN(3), and 2, 4-dinitrophenol (compounds that affect bacterial membrane permeability, sulfhydryl enzymes, and adenosine triphosphate synthesis). Uptake was inhibited by 1% NaCl but was not affected by 10(-3)m ethylenediaminetetraacetic acid disodium salt (a chelating agent for many metallic ions).


Resistance of Bacillus subtilis spores to inactivation by gamma irradiation and heating in the presence of a bactericide. 3. Factors affecting rates of inactivation by phenylmercuric nitrate. Aqueous suspensions of Bacillus subtilis NCTC 8236 spores, surviving gamma irradiation from a cesium-137 source, exhibited an enhanced rate of inactivation compared to nonirradiated spores when heated with 0.04% phenylmercuric nitrate. The enhanced rate of inactivation, observable from survival curves, was noted when spores were irradiated with 150,000 rad under air in either the presence or absence of the bactericide. The magnitude of the enhanced inactivation rate increased as the irradiation dose under air increased from 150,000 to 300,000 rad. The inactivation rates of spores surviving irradiation with 150,000 rad under either oxic or anoxic conditions did not exhibit a simple quantitative relationship. The enhancement effect was observed when the severity of the heat treatment was increased by either reducing the pH from 8 to 6 or raising the temperature from 70 to 90 C.


Immunodiffusion analysis of isolates of Xanthomonas cyamopsidis. Immunodiffusion analysis of intact, sonically treated, and sonically treated and heat-treated cell antigen preparations of isolates of races 0 and 1 of Xanthomonas cyamopsidis of guar, Cyamopsis tetragonoloba, indicated that these races differ from one another antigenically. The differentiating precipitin reactions are believed to have been brought about by specific heat-stable surface antigens, possibly similar to lipopolysaccaride somatic antigens of other slime-producing xanthomonads. Because differences in host reaction to inoculation with the two isolates of race 1 were known to be minor, these isolates are considered to represent serotypes of the race rather than distinct separate races. This conclusion is supported by the antigenic behavior of isolates 14 and 19 to antiserum 19. The applicability of immunodiffusion analysis for the study of pathogenic variability in X. cyamopsidis is demonstrated.


Microagglutination procedures for febrile agglutination tests. Febrile agglutination tests were done by using as antigens Brucella abortus, Salmonella group D, Proteus OX19, and Pasteurella tularensis. Comparison of results from 23 sera showed that the microtechnique, rapid slide, and test tube methods gave similar titers, although those from the microtechnique were generally higher. The sensitivity of the microtechnique depended upon the concentration of antigen, and, to obtain reproducible results, the optimal concentration of antigens had to be determined by preliminary titrations against specific, positive control antisera. Readability of reactions in the microtechnique was enhanced by adding the dye Safranin O to diluent for antigen and by use of V-type, rather than U-type, microtiter plates. Tests were also done to determine the effects of dye and salt concentrations, pH, and temperature of incubation upon the titer of agglutinations by the microtechnique. Our results indicated that the microtechnique could be used for agglutination tests involving febrile antigens. The procedure is less time-consuming than the tube method and requires less antigen and serum than the latter method or the rapid slide method.


Disc plate method of microbiological antibiotic assay. I. Factors influencing variability and error. Several factors are investigated that normally cause variation in zone diameters in conventional disc plate diffusion assay procedures. Of these factors the most serious is the unequal exposure of the individual plates at top or bottom of stacks to temperatures above and below room temperature. This unequal temperature exposure is avoided by novel handling and incubation procedures. A major variable, but one which can be controlled, is the varying time interval between pouring seeded agar and the time of applying the pads with antibiotic to the plates. This influence of time of setting and the effects of several other sequential operations are combined into a composite variable. This variable is then accounted for and normalized by interposing "external" reference plates set with a reference solution in the sequence of approximately 100 plates. No "internal" reference zones are employed. Such factors as volume of agar poured, wedge shape of agar in a dish, volumetric errors in dilutions, and timing considerations are studied and discussed. The results of this study form the basis for a test protocol which is presented in a following paper.


Disc plate method of microbiological antibiotic assay. II. Novel procedure offering improved accuracy. A detailed disc plate procedure is introduced for assay of antibiotics. The procedure is based on a previous study by the authors and deviates from conventional procedures in several respects: selected plastic petri dishes are employed; critical temperature control is simply provided at all stages of the test with refrigeration of the plates never used; all dilution is done with displacement microburettes; six pads (6.3 mm diameter) per dish are employed, all filled with the same unknown or reference solution; the sequence of all plates handled on 1 day is made a part of the protocol which allows accounting for the influence of the order of pouring and setting the plates; external reference plates are set at specified locations in the sequence; and, by averaging the diameters of all zones on a plate, most of the consequence of wedge shape of agar in plates, which is common and almost unavoidable, is removed. The present method is economical, uses simple facilities, and provides good accuracy of test results. Bacillus subtilis was most commonly employed, but other organisms may be employed in the present procedure.


Isolation of echoviruses with human embryonic lung fibroblast cells. More echovirus isolants were obtained from clinical specimens with a strain of human embryonic lung fibroblast cells (RU-1) than with primary rhesus monkey kidney cells.


Small size rubella virus antigens and soluble immune complexes: analysis by the platelet aggregation technique.


Synthesis of virus-specific RNA in cells infected with two different variants of measles virus.


Characterization and transmission of an agent producing a neurological disease in infant mice (IMNV).


Measles antibodies detected by platelet aggregation and gel precipitation in patients with subacute sclerosing panencephalitis.


Effect of nucleotides and nitroglycerine on the hemodynamics of the isolated perfused rat liver.


The involvement of lecithin and monogalactosyl diglyceride in linoleate synthesis by green and blue-green algae.


Phospholipase C from Pseudomonas fluorescens.


Osteomyelitis of the patella. Gentamicin therapy associated with encephalopathy.


Glossoptotic hypoxia and micrognathia--the Pierre Robin syndrome reviewed. Early recognition and prompt surgical treatment is important for survival.


Cephalopod lens proteins.

Cephalopod lens proteins.


Scanning electron microscopy of Schlemm's canal.


The influence of non-ionic detergents and other surfactants on human corneal permeability.


The effect of pH on the multiplication of a pseudomonad in chlorhexidine and cetrimide. Pseudomonas cepacia (Ps. multivorans) can become adapted to a 1 in 30 dilution of Savlon hospital concentrate in distilled water, and can multiply in it. The organism so adapted did not survive in Savlon of the same strength made with hard tap water (pH 7.2). This effect was due to the difference of pH between the two solutions. At the pH of the distilled water solution (pH 6.0) Savlon also showed reduced activity against Proteus vulgaris, but activity against Ps. aeruginosa was not affected.


A mortality study of sickle cell anaemia in Central Africa.


Ankylosis of the stapedio-vestibular joint.


Deafness--loss or disorder.

Deafness--loss or disorder.


Treatment and results in malignant lymphoma.


Congenital lipomatosis: a report of four cases.


Experimental study of splenic trauma in monkeys.


Role of autolytic enzymes in the growth and morphogenesis of bacterial cell walls.


The enigma of the maternal spiral artery and its relation to the fetal cotyledon.


Ocular pathology in triploidy (69-XXY).

Ocular pathology in triploidy (69-XXY).


Survival of gram-negative bacilli and Candida albicans in hexachlorophene preparations and other disinfectants.


Preliminary report. Hepatitis-associated antigen (HAA) in polar areas.


Carbonic anhydrase: a new method of detection on polyacrylamide gels using low-temperature fluorescence.


Analysis of purified tRNA species by polyacrylamide gel electrophoresis.


A method for the determination of disodium cromoglycate and other chromones.


Quantitative ultramicroanalysis of amino acids in the form of their DNS-derivatives. IV. Some applications of the method. Quantitative evaluation of DNS-peptide maps.


Antigenicity of sheep cortical-bone matrix proteins.


Cell fusion without viruses.

Cell fusion without viruses.


Abnormal segregation of a haemagglutinating gene in a control flock.


The binding of copper by bile and serum.


Lymphocyte stimulation by a glycopeptide isolated from Streptococcus pyogenes C203S. I. Isolation and partial purification.


The IgA antibody system.

The IgA antibody system.


Serum immunoglobulins in bovine brucellosis.


Atherosclerosis induced in hypercholesterolaemic baboons by immunological injury; and the effects of intravenous polyunsaturated phosphatidyl choline.


Evaluation of a soluble antigen vaccine prepared from bovine viral diarrhea-mucosal diseases virus-infected cell cultures.


Susceptibility of germfree neonatal cats to Rauscher murine leukemia virus.


A low-molecular weight protein of bovine colostrum similar to immunoglobulin G.


Anophthalmos: an inherited trait in a new stock of guinea pigs.


Egg yolk antigens and their effect on fertility in rabbits.


The electrophoretic separation of human  -galactosidases on cellulose acetate.


The proportion and structure of cells forming antibody, gamma G and gamma M immunoglobulins, and gamma G and gamma M antibodies.


The relationship of delayed hypersensitivity to acquired antituberculous immunity. I. Tuberculin sensitivity and resistance to reinfection in BCG-vaccinated mice.


The relationship of delayed hypersensitivity to acquired antituberculous immunity. II. Effect of adjuvant on the allergenicity and immunogenicity of heat-killed tubercle bacilli.


Genetic aspects of skin and limb development.


Factors regulating biosynthesis, intracellular transport and secretion of amylase and lipase in the rat exocrine pancreas.


Disodium cromoglycate (INTAL).

Disodium cromoglycate (INTAL).


Effect of glibenclamide on hepatic fructose metabolism.


Evidence for complement (C4) on electrophoresis of serum fractions in acid-urea starch-gels.


Periodonal disease: are plasma cells and lymphocytes an essential component?


The simian malarias: zoonoses, anthroponoses, or both?


Deep vascular schizogony of Plasmodium knowlesi in Macaca mulatta. Distribution in organs and ultrastructure of parasitized red cells.


Rickettsioses of Central America.

Rickettsioses of Central America.


Experimental infections of lambs with Rickettsia prowazeki.


Separation of dengue strains on the basis of a nonstructural antigen.


Cutaneous comparative biology.

Cutaneous comparative biology.


Response of the epidermal melanocyte to minor trauma.


Albright's hereditary osteodystrophy with cutaneous bone formation.


Cutaneous ossification in pseudohypoparathyroidism.


Organochlorine compounds in man and his environment.


Effects of reserpine on the social behavior of rhesus monkeys. Reserpine was administered daily by intubation for 81 days to 3 rhesus monkeys to investigate a possible relationship with depression.  Their behavior during the experimental period was compared to their behavior before and after the drug period, as well as to that of a control group of 3 monkeys given water instead of reserpine.  Experimental findings were as follows: 1) Reserpine caused significant behavioral changes in the rhesus monkey.  These changes included decreases in visual exploration and locomotion, and increases in self-huddling, posturing, and tremor; and 2) the behavioral effects of repeated daily dosage were not cumulative nor was tolerance developed by the  drugged monkeys.  There was no visible effect 15 hours after each daily drug administration.  The behavioral alterations were not detectable more than 15 hours following each daily dose.  It was noted that the data were insufficient to justify the conclusion that reserpine administration produced depression in the monkey subjects, even though it did have marked behavioral effects on them.


National health insurance and hospital administration.


Clinical significance of acute changes in the oxyhemoglobin dissociation curve.


Fatal hyperalimentation syndrome. Animal studies.


Hypersensitivity to enzyme additives in laundry detergent workers.


Immunologic distinction of mycobacterial beta-lactamase.


A study of the polypeptides of serum lipoproteins in hypo- -lipoproteinemia.


Abnormal growth hormone (GH) secretion in ketosis-prone diabetes.


Distribution of beta-lipoprotein between low-density and very low-density lipoproteins.


Alterations in thyroxine (T 4 ) and tri-iodothyronine (T 3 ) binding to serum proteins by heat.


Lymphocytic choriomeningitis virus RNAs.


In vitro excision-repair of ultraviolet-irradiated transforming DNA from Bacillus subtilis. The biological activity of UV-inactivated Bacillus subtilis DNA is partly restored after incubation with a UV-specific endonuclease from Micrococcus lutens in conjunction with DNA polymerase and DNA ligase, both isolated from Escherichia coli. The restored activity is not further increased by photoreactivation. Pyrimidine dimers are specifically liberated when irradiated DNA is exposed to the three enzymes. None of these effects is observed when pancreatic DNase is used instead of UV-specific endonuclease.


Albumin evolution in frogs: a test of the evolutionary clock hypothesis. Frogs are an ancient group compared to placental mammals. Yet, although there are about as many species of frogs as there are of mammals, zoologists consider that frogs have undergone only limited morphological divergence, while placental mammals have diversified greatly in morphology and way of life. The serum albumins of numerous frog species were compared by the quantitative microcomplement fixation technique. Frogs that are morphologically similar enough to merit taxonomic distinction at only the species level often exhibit differences in the serological properties of their albumins larger than those usually seen between mammals placed in distinct families or suborders. Thus, there seems to be a contrast between albumin evolution and evolution at the organismal level. The large differences between albumins among frogs can be explained by the hypothesis that albumin evolution has proceeded at the same rate in frogs as in mammals.


Immunoglobulin G and free kapa-chain synthesis in different clones of a hybrid cell line. 13 clonal cell lines were isolated from a hybrid cell population established by cell fusion between cloned BALB/c myeloma cells that were resistant to 8-azaguanine and produced immunoglobulin G and free kappa chain and C(67)BL/6n lymphoma cells that were resistant to bromodeoxyuridine and did not produce immunoglobulins. Some of the histocompatibility-2 antigens of both parental cell lines could be demonstrated on all of the hybrid clones. 11 clones synthesized only free kappa chain. Two clones synthesized IgG of BALB/c type, as well as free kappa chain. These clones had higher chromosome number than did the clones that synthesized only kappa chains.


Is Drosophila dAT on the Y chromosome?

Is Drosophila dAT on the Y chromosome? DNA isolated from Drosophila melanogaster embryos contains measurable amounts of dAT, the copolymer of deoxyadenylate and deoxythymidylate. In wild-type (XX,XY) embryos, dAT constitutes 4% of the total DNA. In embryos containing extra Y chromosomes, or even extra portions of Y chromosomes, dAT constitutes as much as 7% of the total. The enrichment for dAT is dependent upon the number of extra short arms of the Y chromosome (Y(S)) or long arms (Y(L)) of the Y chromosome per individual. This enrichment of dAT dependent on the Y chromosome may be interpreted in several ways. The most straightforward interpretation is that dAT is present in high concentrations on the Y chromosome.


Stimulation of antibodies in Trypanosoma evansi infection of rabbits and their disappearance after treatment of the infection.


Evaluation of environmental hazards following the use of synthetic grit containing mestranol for pigeon control.


Toxicologic studies of fenfluramine.

Toxicologic studies of fenfluramine.


Expressivity of heritable telecanthus in five generations of a kindred.


Thymidylate synthetase and its relationship to dihydrofolate reductase.


Levels of dihydrofolate reductase and thymidylate synthetase in strains of Streptococcus faecium resistant to substituted pyrimidines, triazines and MTX.


Confirmation of collateal sensitivity to pyrimethamine in Lactobacillus casei ATCC 7469 resistant to chlorguanide triazine.


Empirical vs. rational approaches in cancer chemotherapy.


Synthesis and biological activity of tricyclic antifolate compounds with fixed geometry.


Deaza aalogues of amethopterin (methotrexate).


Myambutol: chemistry, pharmacology, and toxicology.


The anti-mycobacterial activity of ethambutol (ETB).


Determination of resistance of M. tuberculosis to ethambutol.


Chemical and biological properties of rifampicin.


Rifampicin in experimental investigations on mice.


Antimycobacterial activity of rifampicin.


Experimental and clinical studies on the antituberculous activity of rifampicin alone or combined with other drugs.


Pharmacology and toxicology of Isoxyl.

Pharmacology and toxicology of Isoxyl.


Influence of temperature on substrate and energy conversion in Pseudomonas fluorescens. The influence of temperature on yield, maintenance rate, growth rate, and conversion of calories to biomass was studied with Pseudomonas fluorescens grown in a chemostat. Maintenance and growth rate are influenced linearly with temperature. Both rates increased with increasing temperature and gave linear Arrhenius plots over a limited range. Cells harvested during the steady-state at each temperature were burned in a microcalorimeter. The number of kilocalories per gram (dry weight) of organism was not influenced significantly by the temperature during growth, indicating that the conversion of substrate calories into biomass is apparently regulated in the range of temperature studied.


Bacteriological evaluation of two test methods for chlorine in swimming pools. It was found that orthotolidine and plastic test strips containing a mixture of syringaldazine and vanillinazine gave different results as to chlorine content of water samples containing urine or large amounts of bacteria while giving consistently similar results with water samples having only sodium hypochlorite added. Orthotolidine was found to give erroneously "safe" readings when in fact viable bacteria were observed on membrane filters after filtration.


Staphylococcal enterotoxin B: solid-phase radioimmunoassay. An immunoassay employing (125)I-labeled enterotoxin B and polystyrene tubes coated with specific antibody was used for assaying purified and crude enterotoxin. Antibody was adsorbed to untreated polystyrene tubes. Unlabeled enterotoxin competed with (125)I-labeled enterotoxin for antibody-combining sites. The uptake of (125)I-labeled toxin reflected the concentration of unlabeled toxin present. The test is sensitive to 1 to 5 ng of purified and crude enterotoxin B per ml, and cross-reactions with heterologous enterotoxins did not interfere with the specificity. This test possesses the combination of sensitivity and objectivity absent in current methods for assaying enterotoxin and provides a model for investigating other enterotoxin serotypes.


Therapy of staphylococcal infections in monkeys. VII. Comparison of cyclacillin and nafcillin. Intravenous inoculation of a penicillin-resistant phage type 80/81 staphylococcus caused lethal infection in seven of eight untreated monkeys. Daily intragastric administration of 50 mg/kg given in two equal morning and afternoon doses of cyclacillin and nafcillin was followed by mortalities of four of four and two of four monkeys, respectively. After 100 mg per kg per day, three of four and one of four monkeys receiving cyclacillin and nafcillin, respectively, died. Thus, mortality in controls and cyclacillin-treated monkeys was seven of eight as compared to three of eight after nafcillin treatment. Although the staphylococcus was more resistant to cyclacillin (minimal inhibitory concentration = 7.80 mug/ml) than to nafcillin (minimal inhibitory concentration = 0.31 mug/ml), regular rapid absorption and high levels of the former suggested potential efficacy. However, the similar mortality in cyclacillin-treated and control monkeys indicated that the in vitro data did not, in this instance, conform to the in vivo observations.


Polyacrylamide gel electrophoresis of Corynebacterium diphtheriae: a possible epidemiological aid. In this preliminary study, the use of polyacrylamide gel electrophoresis as an aid in the characterization of Corynebacterium diphtheriae was evaluated and a standardized method was developed. The electrophoretic patterns of 17 gravis, 14 mitis, and 2 intermedius types of C. diphtheriae were compared with the electrophoretic patterns of 5 Robinson and Peeney stock gravis serotype strains. Each of the 5 stock serotype strains had different electrophoretic patterns, although some common bands were present. The 17 gravis strains isolated in the United States showed patterns identical to those of the stock gravis serotype II strain. The 14 mitis strains examined produced 6 different electrophoretic patterns, irrespective of geographical location. One mitis pattern corresponded with the pattern of gravis serological type II. The two intermedius strains examined had identical electrophoretic patterns that resembled the pattern of gravis serotype IV. Polyacrylamide gel electrophoresis of C. diphtheriae strains may prove to be a useful epidemiologic tool in establishing the distribution and occurrence of various C. diphtheriae types.


Enhancing effects of carbon dioxide on the primary isolation of acid-fast bacilli in a modified Lowenstein-Jensen medium. Specimens inoculated on modified Lowenstein-Jensen medium were incubated aerobically and in an atmosphere of 8% CO(2). Sixteen per cent of the positive specimens grew Mycobacterium tuberculosis only after incubation in CO(2).


Changes in histaminase content following experimental thermal injury.


Pseudomonas aeruginosa in a skin hospital.


Osteoma cutis and Albright's hereditary osteodystrophy.


Aluminium hydroxide implants on the inferotemporal cortex of the monkey: their mode of influencing visual discrimination performance.


Somatosensory deficit produced by parietal-temporal cortical disconnection in the monkey.


Production of involuntary movements by L-dopa in monkeys with tegmental lesions.


Topographic projections on the globus pallidus and the substantia nigra of selectively placed lesions in the precommissural caudate nucleus and putamen in the monkey.


Central sites of action and effects of acetylstrophanthidin on body temperature in monkeys.


Natural selection for quantitative characters.


In vitro combined activity of carbenicillin and gentamicin on pseudomonas aeruginosa.


Incidence of toxoplasma antibodies in tuberculous patients.


A simple method for producing monospecific antiserum to a selected fraction of human serum protein.


Antigenic analysis of different strains of Brucella.


Antigenicity and immunopathogenicity of the chemical fractions of heart.


A study on morbidity and drug costs in a primary health centre.


Osteomalacia in neurofibromatosis.

Osteomalacia in neurofibromatosis.


Immunologic analysis by gel diffusion of effects of prolonged cultivation on Histomonas meleagridis (Smith).


A check-list of the species of the genus Haemoproteus (Apicomplexa, Plasmodiidae).


The occurrence of Schellackia and Plasmodium in two Georgia lizards.


Defective micronuclei and genomic exclusion in selected C subclones of Tetrahymena.


The purification of DNA from the genones of Paramecium aurelia and Tetrahymena pyriformis.


Effects of different substrates on glucose uptake and hexokinase activity in Euglena gracilis.


Prevalence of Sarcocystis in grackles in Maryland.


Determination of cyanogen chloride in activated niacin test strips.


Renal effects of massive infusions of adenine during resuscitation from hemorrhagic shock in the baboon.


Alterations in baboon erythrocyte 2,3-diphosphoglycerate concentration associated with hemorrhagic shock and resuscitation.


Nitrogenase activity and nitrogen assimilation in Anabaena flos-aquae growing in continuous culture.


Kinetics of synthesis of nitrogenase in batch and continuous culture of Anabaena flos-aquae.


Studies on the delayed light emission in spinach chloroplasts. I. Nature of two phases in development of the millisecond delayed light emission during intermittent illumination.


Evidence for a two-directional hydrogen ion transport in chloroplasts of Euglena gracilis.


Pyruvate: ferredoxin oxidoreductase and its activation by ATP in the blue-green alga Anabaena variabilis.


Serum proteins of the Japanese quail, Coturnix coturnix japonica.


Maternal and neonatal Australia antigen. Four hundred and fourteen pairs of maternal and cord blood specimens from a county hospital population in southern California were assayed for Australia antigen by complement fixation and immunodiffusion techniques. Four maternal specimens were found to be positive (1 percent). One of the mothers with positive reaction was ill with viral hepatitis at time of delivery. Three had no evidence of symptomatic liver disease during pregnancy; two were born and raised where the prevalence of Australia antigen (Au(1)) is higher than in the United States. Although none of the cord blood specimens were positive for Au(1), one infant whose mother had Au(1) at delivery became positive at one month of age, and positive titers have persisted through 12 months of life. The significance of chronic Au(1) antigenemia in this infant is uncertain. However, it may be of importance in understanding the pathogenesis of viral hepatitis in the neonatal period.


Primate hemoglobins: their structure, function and evolution. I. Amino acid compositions of the tryptic peptides from the beta chain of Cebus albifrons.


Ribosomes of the malarial parasite, Plasmodium knowlesi. I. Isolation, activity and sedimentation velocity.


Ribosomes of the malarial parasite, Plasmodium knowlesi. II. Ultrastructural features.


Observations on the phenoloxidase system in the armored scales Aonidiella aurantii and Chrysomphalus aonidum.


Extrapyramidal pathways with monosynaptic effects upon primate  -motoneurons.


Two types of viruslike particles in Drosophila midgut.


Teratogenic effects induced in tail of Bufo arenarum tadpoles following treatment with carcinogens.


The action of inhibitors of nucleic acids synthesis on Euglena.


The antimicrobial activity of citral.

The antimicrobial activity of citral.


Structures of the venturicidins A and B.


Studies on the decline in immunosuppressive potency of rabbit anti-mouse thymocyte sera raised by repeated immunization of the same rabbit.


A new method for the cytochemical demonstration of p-diphenol: O 2  oxidoreductase (laccase).


Studies on the coronary microcirculation by direct visualization.


Inhibition of ribosomal ribonucleic acid maturation in rat liver by 5-fluoroorotic acid resulting in the selective labeling of cytoplasmic messenger ribonucleic acid.


Dehydrogenation of sterols by the protozoan Tetrahymena pyriformis.


Mechanism of action of guinea pig liver transglutaminase. 8. Active site studies with "reporter" group-labeled halomethyl ketones.


Heat survivor curves of food-borne bacteria suspended in commercially sterilized whole milk. II. Bacteria other then Salmonellae.


Field diagnosis of athletic injuries.

Field diagnosis of athletic injuries.


Studies of carbamate pesticide metabolism utilizing plant and mammalian cells in culture.


Mechanism of the arginine requirement for adenovirus synthesis. I. Synthesis of structural proteins. The mechanism of the arginine requirement for adenovirus was studied in cultures of KB cells infected with adenovirus type 2. Macromolecular synthesis was found to be severely impaired in uninfected cells under complete arginine deprivation, whereas an arginine concentration of 50 mum yielded a moderate and reversible inhibition of growth and nucleic acid synthesis. At this concentration, viral structural proteins were accumulated in excess although the virus yield was reduced more than 1,000-fold. The arginine-sensitive step appeared to occur early during the first 15 hr postinfection in the virus growth cycle. Virus-infected cells deprived of arginine to 50 mum showed, when reversed, a 4- to 5-hr lag period before the increase in virus growth was observed. Analysis of the radioactive pattern of labeled virions synthesized after reversion showed that all polypeptides were synthesized after addition of arginine to the medium, and none of the virion-polypeptides which are revealed by gel electrophoresis appeared to be preferentially synthesized after arginine reversion. The excess pool of structural proteins formed during depletion appeared to a large extent to be unavailable for virus assembly.


Influence of herbicides on insecticide metabolism in leaf tissues.


Insecticide inhibition of herbicide metabolism in leaf tissues.


Effect of carbonic anhydrase on glyceraldehyde-3-phosphate dehydrogenase activity.


Advances in allergy.

Advances in allergy.


Long term management of the hemophilic patient with emphasis on musculoskeletal complications.


Metabolic alterations in the squirrel monkey induced by DDT administration and ascorbic acid deficiency.


The immune response of the host: an aid to etiology, pathogenesis, diagnosis, and epidemiology of bacterial infections.


Pathogenic significance of Pseudomonas fluorescens and Pseudomonas putida.


Degenerative changes in the optic terminals in the lateral geniculate nucleus of the monkey.


Use of urea in the chromatography of transfer RNA on reversed-phase column RPC-2.


Investigation of a drug possibly implicated in sporadic jaundice using human liver cells in culture.


Interaction of informational macromolecules with ribosomes. IV. Dissociation of RNA-binding ability from ribosomes by treatment with salts.


Biochemical and pharmacologic studies with 1- -D-arabinofuranosylcytosine 5'-adamantoate (NSC-117614), a depot form of cytarabine.


The use of modified cells to induce an anti-Rh response.


On the structure and function of Y protein.


Some recent studies on species variations in drug metabolism.


Modification of recombination frequency in Drosophila. I. Selection for increased and decreased crossing over.


Modification of recombination frequency in Drosophila. II. The polygenic control of crossing over.


Elongate and round gametocytes of Leucocytozoon simondi (Mathis & Léger) in ducks inoculated with megaloschizonts.


Considerations in the development of total intravenous feedings.


A microsporidan hyperparasite of strigeoid trematodes, Nosema strigeoideae sp. n.


Clinicopathological considerations in spinal meningeal calcification and ossification.


Neurologic involvement in Urbach-Wiethe's disease (lipoid proteinosis). A clinical, ultrastructural, and chemical study.


Alcoholism and 'petit mal fits'.

Alcoholism and 'petit mal fits'.


Clock mutants of Drosophila melanogaster. Three mutants have been isolated in which the normal 24-hour rhythm is drastically changed. One mutant is arrhythmic; another has a period of 19 hr; a third has a period of 28 hr. Both the eclosion rhythm of a population and the locomotor activity of individual flies are affected. All these mutations appear to involve the same functional gene on the X chromosome.


Determination of blastoderm cells in Drosophila melanogaster. A method for culturing blastoderm cells of Drosophila in vivo has been developed that allows these cells to differentiate into larval or adult structures. By intermixture of genetically marked cells from bisected and whole embryos, it was shown that blastoderm cells are restricted in their potential for forming adult epidermal structures. Cells isolated from anterior-half embryos are determined for forming head and thoracic structures, whereas cells from posterior-half embryos are determined for forming thoracic and abdominal structures. The specificity of determination and the localization of determinative factors is discussed.


Genetic analysis of cell heredity in imaginal discs of Drosophila melanogaster. Somatic crossing-over was used in heterozygous Drosophila melanogaster to effect clonal homozygosity for three mutants (h, Hw, ac). In these mutants, one type of process, chaetae, replaces another type, trichomes, in specific patterns on the adult fly. Heterozygous individuals were irradiated at different larval or pupal ages with x-rays and the somatic crossing-over was identified in the adult cuticle by genetically coupled mutants serving as cell markers of chaetae and trichomes. Induction of homozygosis more than 8 hr before puparium formation resulted in autonomous differentiation of the mutant pattern in the homozygous patch of tissue for the three mutants tested; homozygosis induced within 8 hr of puparium formation was not followed by expression of the corresponding genotype. 8 hr before puparium formation, each cell type still has to divide twice before metamorphosis and differentiation. Thus, the genetically conditioned decision of a specific cell to differentiate either a chaeta or a trichome is made during the growth of the wing imaginal disc and is transmitted clonally to descendant cells. Once this decision has been made, subsequent changes in the genotype of the cell or of its daughters are not material to the fate of the cell. We introduce the term "perdurance" to designate the persistence of a cellular developmental fate for several cell generations after the loss of the genetic basis for that cellular development.


Sequence differences of Escherichia coli 30S ribosomal proteins as determined by immunochemical methods. Antisera specific for each of the 21 homogenous ribosomal proteins from 30S subunits of Escherichia coli were used to investigate, by immunodiffusion and immunoprecipitation, whether there are any extensive sequence homologies among these proteins. No immunological crossreactions were detected between any of the proteins. Therefore, we conclude that no significant common sequences exist among any of the 21 30S ribosomal proteins of E. coli.


Synthesis and degradation of liver acetyl coenzyme A carboxylase in genetically obese mice. The total cytosol activity of acetyl-CoA carboxylase (acetyl-CoA:CO(2) ligase (ADP), EC 6.4.1.2) in the liver is known to be 6- to 10-fold higher in genetically obese hyperglycemic mice (C57BL/6J-ob) than in nonobese mice. The results of immunochemical titrations, Ouchterlony double-diffusion analysis, and kinetic and heat inactivation studies indicated that this rise in the level of carboxylase activity in liver extracts from obese mice was ascribed to an increase in the quantity of the enzyme protein, which was indistinguishable from that derived from nonobese mice. Combined immunochemical and isotopic techniques showed that the rate of synthesis of the carboxylase per liver was 7.7-fold higher in obese than in nonobese mice. The rate of degradation of the carboxylase was found to be 1.7-fold lower in obese than in nonobese mice, the half-life being 115 and 67 hr, respectively. These results indicate that the increase in the acetyl-CoA carboxylase content of the liver in obese mice is due mainly to a rise in the rate of enzyme synthesis, and in a minor degree, to a decrease in the rate of enzyme degradation.


The prevention of rhesus immunization.

The prevention of rhesus immunization.


Plasma levels and urinary excretion of disodium cromoglycate afer inhalation by human volunteers.


Acute toxicity of aflatoxin B 1  in the macaque monkey.


Polychlorinated biphenyls as inducers of hepatic porphyria in Japanese quail, with special reference to   -aminolevulinic acid synthetase activity, fluorescence, and residues in the liver.


Action of diastereoisomers of tetrahydrohomofolate on the growth of Lactobacillus casei.


Discussion paper: amethopterin linked covalently to water-soluble macromolecules.


Folate antagonists as antibacterial and antiprotozoal agents.


New approaches to chemotherapy.

New approaches to chemotherapy.


Circular dichroism studies on the variable and constant halves of kappa-type Bence-Jones proteins.


The effect of dextranase on caries activity in monkeys (Macaca irus).


Bacillus cereus neutral protease.

Bacillus cereus neutral protease.


The effect of visible light on the biological activity of mitomycin C in bacteria.


Fluorescent probes of biological macromolecules. In: Molecular properties of drug receptors.


Serine biosynthesis in Tetrahymena pyriformis S.


Effect of intra-articular injection of hyaluronic acid on the clinical symptoms of osteoarthritis and on granulation tissue formation.


Isometric contraction in glycerinated skeletal muscle of horseshoe crab and rabbit. 3. Relaxation.


An electrophoretic study of lactate dehydrogenase isoenzymes, protein and lipoprotein of Drosophila melanogaster larvae, pupae and adults.


The cause of vomiting of tube feedings by neurosurgical patients.


Brain motion during vibration of water immersed rhesus monkeys.


Susceptibility of the gallinaceous birds to Akiba caulleryi.


DNA-protein complex in circular DNA from phage phi-29.


A controlled comparison of drug effects on escape from conditioned aversive stimulation ("anxiety") and from continuous shock.


Suppression of urethan-induced lung adenomas in mice treated with trehalose-6,6-dimycolate (cord factor) and living bacillus Calmette Guérin. Trehalose-6,6-dimycolate (cord factor), a glycolipid from mycobacteria, suppressed the development of urethan-induced tumors in the lungs of mice to a similar degree as living Mycobacterium bovis (strain BCG) bacilli. The inhibition was apparently due to the host cellular reaction caused locally by cord factor.


Catabolism of L-arginine by entrapped cells of Streptococcus faecalis ATCC8043.


The contamination of laboratory animals with lymphocytic choriomeningitis virus.


Studies on phytohemagglutinins. IX. Metal content and activity of the hemagglutinin from the lentil (Lens esculenta Moench).


Anomalous energy transfer behaviour of light absorbed by bacteriochlorophyll in several photosynthetic bacteria.


Nature of vitamin B 12  binding by native serum, seromucoid, and highly purified fractions from chicken serum.


Disproportionation of  I,5 -diphenylcarbazone. A new reaction catalysed by photosystem I.


Health insurance: the Canadian experience.


Prediction of total body potassium from anthropometric measurements.


Plasma globulin determinations in patients with microglioma. Report of five cases.


Ultrastructural identification of non-adrenergic inhibitory nerve fibers.


A comparison of pentobarbital and cocaine self-administration in rhesus monkeys: effects of dose and fixed-ratio parameter.


Aversive properties of nalorphine and naloxone in morphine-dependent rhesus monkeys.


Metabolism of a new hypolipidemic agent, 2-acetamidoethyl(p-chlorophenyl) (m-trifluoromethylphenoxy)-acetate (halofenate) in the rat, dog, rhesus monkey and man.


Autoimmune orchitis induced by autoimmunization with seminal plasma in the rabbit.


Volatile constituents of primate vaginal secretions.


Observations on liver function in hematological patients with Australia antigen or antibody in the serum.


Surgical treatment of chronic infectious pulmonary tuberculosis without antituberculous chemotherapeutic cover.


Immunopathology.

Immunopathology.


Basic A1 protein of myelin: relationship to experimental allergic encephalomyelitis.


Relationship of experimental allergic encephalomyelitis to human disease.


Urinary protein components. I. Basis and general methodology.


Thyroid antigens and autoimmunity.

Thyroid antigens and autoimmunity.


Lung changes after beryllium inhalation: ultrastructural and morphometric study.


Patient incentives and hospital insurance: a political science perspective.


Replication of CELO virus in the chicken chorio-allantoic membrane.


Further investigations of circulating antibodies in colon cancer patients: on the autoantigenicity of the carcinoembryonic antigen.


The relative importance of blood monocytes and fixed macrophages to the expression of cell-mediated immunity to infection. Infection in mice with Listeria monocytogenes results in a substantial accumulation of migrant macrophages in the liver. The immigrant cells populate both the infective foci and intervening sinusoids. They have the labeling characteristics of blood monocytes, and their appearance in infective foci in the liver corresponds to the expression of a high level of antimicrobial immunity in this organ. The infected liver acquires additional new macrophages by Kupffer-cell division. The proliferation of these cells, however, is not essential for the expression of immunity in the liver. The results indicate that the macrophages which express immunity to a primary infection with L. monocytogenes are those derived from circulating monocytes. Most of these cells are quickly lost once the parasite is eliminated from the tissues.


Temporal development of resistance to pulmonary tuberculosis in Swiss albino mice. The course of pulmonary tuberculosis was followed in control and BCG-vaccinated mice. Resistance was evaluated by determining the numbers of virulent mycobacteria recovered from the organs at various intervals after airborn infection. A limited but significant retardation of growth of challenge bacilli was observed in both pulmonary and splenic tissues of successfully vaccinated animals. This retardation was manifested chiefly during the early stages of the infection. However, the numbers of virulent organisms recovered from the organs of either vaccinated or control animals were much the same at later stages of the infection. The appearance of pulmonary immunity was related to the stage of development in vivo of the vaccinal population. Only after large numbers of vaccinal bacilli could be recovered from the tissues was heightened resistance observed.


The mediator of cellular immunity. I. The life-span and circulation dynamics of the immunologically committed lymphocyte. Thoracic duct cells from rats which have survived an infection with Listeria monocytogenes can confer a high level of antimicrobial resistance upon normal recipients. The cells which confer protection appear in the thoracic duct during the 1st wk of the immunizing infection, at a time when newly formed lymphocytes are being added to the lymph in substantially increased numbers. The protective cells differ in at least two respects from the majority of small lymphocytes in central lymph: they have a rapid turnover rate and a short life-span in the circulation. Evidence was also obtained that lymphopoiesis affecting the long-lived small lymphocyte, which belongs to the recirculating pool, is not increased during an acute Listeria infection.


The mediator of cellular immunity. II. Migration of immunologically committed lymphocytes into inflammatory exudates. Peritoneal exudates from rats which have survived an infection with L. monocytogenes can protect cyclophosphamide-treated recipients against a Listeria challenge. They are more effective in this respect than cells obtained from the spleen or thoracic duct lymph. Since exudate cells from normal rats and inocula prepared from the resident peritoneal cell populations of infected donors are unable to inhibit the challenge infection, the protective cells must belong to a class of lymphocytes that emerges from the blood in response to inflammation. It is significant therefore that thoracic duct lymphocytes formed during an acute Listeria infection can move into exudates induced by a variety of inflammatory stimuli. The affinity of newly formed lymphocytes for inflamed tissue points to a mechanism whereby the host marshalls its cellular defenses at sites of bacterial invasion. The tendency of short-lived lymphocytes to leave inflamed vessels might also explain their short-circulating life-span.


The fine structure of tick stages of Theileria parva.


Syngamy and sporogony of Hepatozoon griseisciuri Clark, 1958 (Sporozoa:Haemogregarinidae), in its natural vector, Haemogamasus reidi Ewing, 1925 (Acari: Mesostigmata).


Studies on the transmission of simian malaria. IV. Further studies on the transmission of Plasmodium knowlesi by Anopheles balabacensis balabacensis mosquitoes.


Aspects of nitrate ester metabolism.

Aspects of nitrate ester metabolism.


Influence of formulating factors on drug safety of timed-release nitroglycerin tablets.


Thermoregulation around a new  set-point' established in the monkey by altering the ratio of sodium to calcium ions within the hypothalamus. 1. The cerebral ventricles of the unanaesthetized monkey were perfused at a rate of 50-100 mul./min with artificial cerebrospinal fluid (c.s.f.) containing 11.0-34.0 mM excess sodium or 23.9-47.9 mM excess calcium ions. By repeated perfusions, a marked hyperthermia elicited by excess sodium or deep hypothermia by excess calcium was sustained for up to half a day.2. During the sodium-induced hyperthermia or calcium-induced hypothermia, the monkey thermoregulated adequately around the new level of temperature in response to either 50 or 150 ml. water heated to 58 degrees C or chilled to 0 degrees C and given by the intragastric route. Cold water produced a transient hypothermia after which temperature returned to the pre-load level; hot water given in the same way evoked a short-lasting hyperthermia, and again the temperature returned just to the new setpoint level.3. Perfusions at 50 mul./min of isolated areas of the diencephalon by means of push-pull cannulae with a solution containing 11.0-34.0 mM excess sodium ions or 11.3-47.9 mM excess calcium ions altered the body temperature of the monkey only when the sites of perfusion were located in the mammillary region of the posterior hypothalamus. Following the prolonged hyperthermia produced by excess sodium ions or the deep hypothermia evoked by excess calcium ions in the push-pull perfusion fluid, the monkey thermoregulated around the new temperature level again in response to 150 ml. of either hot or cold water given in the stomach.4. Chelation of calcium ions within the ventricles by perfusing EGTA through the cerebral ventricles caused a long-lasting elevation in temperature. When EGTA was perfused directly in the posterior hypothalamus by means of push-pull cannulae, a profound hyper-pyrexia ensued which could be abolished immediately by perfusing a solution of excess calcium at the same diencephalic site.5. During the calcium-induced hypothermia, the heart rate declined but the electrical activity of the cortex was relatively unchanged. Feeding was also elicited in the hypothermic monkey by noradrenaline microinjected at a site in the hypothalamus at which eating was evoked when the body temperature was normal.6. The evidence supports the hypothesis that in the primate, the inborn mechanism which establishes the set-point for body temperature at 37 degrees C is the constancy in the intrinsic ratio between sodium and calcium ions within the posterior hypothalamus. If the set-point is elevated or lowered by a disturbance of the balance between these two cations, the monkey nevertheless can thermoregulate normally around the new level of body temperature.


General pharmacological actions of some synthetic tetrahydrocannabinol derivatives.


Evaluation of a polydipsia technique to induce alcohol consumption in monkeys.


Effects of prefrontal cortex ablation on two-bar ratio performance in primates.


Parenteral alimentation and regulation of energy balance in rat.


Isolation of a paravaccinia virus from bovine semen.


Nuclear proliferation and the amount of DNA per parenchymal nucleus during mouse liver regeneration.


Unusual findings in the human renal glomerulus in multiple myeloma. A light- and electron-microscopic study.


Effect of heparin on cell population kinetics, mitosis and topoinhibition.


Distribution and turnover of a cytotoxic depigmentary agent (hydroquinone) in black goldfish, Carassius auratus L., and consideration of the mechanism of action.


A cytophotometric study of benign and malignant phaeochromocytomas.


Evaluation of the hypothalamus-pituitary-adrenocortical function in young healthy and diarrhoeic calves by means of insulin hypoglycaemia.


OPT-induced fluorescence of glucagon and secretin.


Insect brain as a model for the study of aging. Age-related changes in drosophila melanogaster.


Segregation of membrane markers during cell division in Escherichia coli. II. Segregation of Lac-permease and Mel-permease studied with a penicillin technique.


Effects of calcium and lead on potassium permeability of human erythrocyte ghosts.


Enzymes of phospholipid metabolism: localization in the cytoplasmic and outer membrane of the cell envelope of Escherichia coli and Salmonella typhimurium.


The interrelation of polar lipids in bacterial membranes.


Small molecular weight RNA components in Tetrahymena pyriformis.


Tectal projection to the posterior thalamus of the squirrel monkey.


Recovery of function following prefrontal lobe damage in rhesus monkeys.


Representation of the visual field in striate and adjoining cortex of the owl monkey (Aotus trivirgatus).


The fate of N-alkyl groups in the course of binding of metabolites of several N-alkyl amino azo dyes to rat-liver proteins.


The synthesis of model compounds for the polar dyes derived from livers of rats given 4-amino-2',3-dimethyl-azobenzene.


The role of the olfactory signals in the mating behavior of bonnet monkeys (Macaca radiata).


Kidney function in experimental diabetic ketosis.


Activation of subtilisin by luteotropic and luteinizing hormone.


The lipids of hydrogen-oxidizing bacteria: occurrence of cis-9,10-methylene hexadecanoic acid in Hydrogenomonas H 16.


On the relative position of the centromere of chromosome 3 in Drosophila melanogaster.


Enhanced survival of germfree mice after infection with irradiated scrapie brain.


Fiber systems of medial and anterior thalamus of Macaque.


Characteristics of the human skin bradykinin-destroying enzyme.


The role of recombination in transfection of B. subtilis.


Coagulation studies in acute hepatic necrosis. Effects on coagulation of extracorporeal liver perfusion and exchange blood transfusion.


Severe temporary deterioration of renal transplant function following meningococcemia.


The hypothalamic hypophysiotropic thyrotropin-releasing factor.


The excretion of steroid hormone metabolites in bile and feces.


Catalytic versatility of erythrocyte carbonic anhydrase. IX. Kinetic studies of the enzyme-catalyzed hydrolysis of 3-pyridyl and nitro-3-pyridyl acetates.


Purification and properties of carbonic anhydrase from sheep erythrocytes.


Gonococcal tonsillar infections.

Gonococcal tonsillar infections. Neisseria gonorrhoeae were cultured from tonsillar swabs in six men and six women out of 161 consecutive, unselected, Danish patients (95 men and 66 women) suffering from urogenital or rectal gonorrhoea. Gonococci were found in the tonsils in only one out of 49 foreign men with gonorrhoea. Eleven of the Danish patients admitted orogenital contact at their most recent intercourse. The gonococcal complement fixation test was negative in all except two cases. Standard singledose treatment cured the urogenital and rectal infections promptly but failed to cure the tonsillar infection in five cases, and in recalcitrant cases the organisms were demonstrable for some months.


Extrageniculostriate vision in the monkey. II. Demonstration of brightness discrimination.


The effects of impairment of afferent feedback from the moving limb on the natural activities of neurones in the precentral gyrus of conscious monkeys: a preliminary investigation.


An improved fractionation system for pronase on CM-sephadex.


Injections of GABA and glutamate into the amygdalae of awake monkeys.


Regeneration and duplication following operations in situ on the imaginal discs of Drosophila melanogaster.


On the aneurysms of a. lienalis in liver cirrhoses.


South American trypanosomiasis (Chagas' disease).


Substituent effect on the hydrolyses of phenyl  -maltosides catalyzed by saccharifying  -amylase from Bacillus subtilis.


Studies on bacterial proteases: dye-sensitized photooxidation of neutral subtilopeptidase amylosacchariticus.


Recent investigations on Pseudomonas aeruginosa.


Purification of snail hemagglutinin from Helix pomatia and its serological characteristics.


Nonspecific protective properties of Pseudomonas aeruginosa endotoxin and its components.


Property to induce interferon of Pseudomonas aeruginosa endotoxin and its components.


Spontaneous rupture of an oesophageal diverticulum.


Socio-economic evaluation.

Socio-economic evaluation.


Roentgen alterations in the gastrointestinal tract in immunoglobulin abnormalities.


Platelet interaction with bacteria. II. Fate of the bacteria. Several common strains of bacteria have been studied to determine their influence on human and rabbit platelets in vitro. Bacteria at a nominal ratio of 1:1 were added to platelets in their native plasma or to platelets in a balanced salt solution. The platelet-bacterial interaction was examined by nephelometry and by sequential light microscopy of fixed specimens taken at key points in the reaction. The results support the following conclusions: (1) Bacteria that were potent stimuli of the platelet aggregation reaction were incorporated in large numbers into the forming platelet aggregates. (2) Bacteria sequestered within platelet aggregates remained in direct contact with the aggregated platelet mass for up to 1 hour in vitro and during this time showed no morphologic changes by light microscopy. (3) Plateletbacterial interaction produced clumping of the bacteria but no demonstrable killing of the microorganisms. (4) Collagen-platelet interaction resulted in release of platelet products that were capable of clumping bacteria but were not lethal to them. (5) Rabbit and human platelets showed no species differences in plateletbacterial interaction. (6) Washed platelets interacted with bacteria without added serum factors but the addition of serum accelerated the aggregation reaction.


Cost of tests.

Cost of tests.


Corticofugal connections of area 8 (frontal eye field) in Macaca mulatta.


Guidance of neurons migrating to the fetal monkey neocortex.


Firing patterns of reticular formation neurons during horizontal eye movements.


Hydrogen isotope exchange in the study of protein conformation. A quantitative test of an exchange mechanism.


Interaction of nalidixic acid with Klebsiella (Aerobacter) aerogenes growing in continuous culture.


Hydroxylamine and derivatives: cytotoxicity without mutagenicity in cellular genetic systems.


The changing pattern of carcinogenic aminoazo dye-binding proteins during the course of continuous feeding of 3'-methyl-4-dimethylaminoazobenzene.


Protective isolation in a burns unit: the use of plastic isolators and air curtains. The use of plastic isolators and of an ;air curtain' isolator for protection of patients against infection was studied in a burns unit.Preliminary bacteriological tests showed that very few airborne bacteria gained access to a plastic ventilated isolator; even when the filter and pre-filter were removed from the air inflow, settle-plate counts inside the isolator were much lower than those in the open ward, but the difference was smaller in tests made with an Anderson air sampler, which showed also that fewer large bacteria-carrying particles appeared inside the isolator than outside it. An open-topped isolator allowed virtually free access of bacteria from ambient air. The numbers of airborne bacteria inside an air curtain were appreciably lower than the counts of airborne bacteria in the open ward, but not as low as those in the plastic ventilated isolator.Controlled trials of isolators were made on patients with fresh burns of 4-30% of the body surface; the patients were given no topical chemoprophylaxis against Staphylococcus aureus or Gram-negative bacilli. Patients treated in plastic isolators showed a significantly lower incidence of infection with Pseudomonas aeruginosa than those treated in the open ward; this protective effect was shown by isolators with or without filters or with an open top. Ventilated isolators, which protected patients against personal contact and airborne infection, gave a limited protection against multi-resistant ;hospital' strains of Staph. aureus, but no such protection was given by an open-topped isolator, which protected only against personal contact infection, or by air curtains, which protected only against airborne infection; the air curtain gave no protection against Ps. aeruginosa, and there was no evidence of protection by any isolator against Proteus spp. and coliform bacilli.Both the controlled trials and evidence from the bacteriology of air, hands, fomites and rectal and nasal swabs taken on admission and later, supported the view that Ps. aeruginosa is transferred mainly by personal contact, Staph. aureus probably by air as well as by contact and coliform bacilli mainly by self infection with faecal flora, many of which are first acquired from the hospital environment in food or on fomites.The use of plastic isolators is cumbersome, and of limited value except in the control of infection with Ps. aeruginosa. For this reason and because of the effectiveness of topical chemoprophylaxis such isolators are unlikely to have more than an occasional use in the treatment of burns. Though air curtains greatly reduce airborne contamination, their use in a burns unit does not appear to protect patients against infection when the alternative (and, for Ps. aeruginosa, more important) routes of contamination by personal contact and fomites are left open.


The Australia (hepatitis-associated) antigen amongst heroin addicts attending a London addiction clinic. Thirty-three of 72 heroin addicts attending a recognized clinic for drug addition had a history of jaundice, but in only five was the serum positive for hepatitis-associated antigen (HAA) when examined by immunodiffusion, immunoelectro-osmophoresis and complement fixation. Two of these were repeatedly positive over an 8-12 month follow-up period and liver biopsy showed chronic persistent hepatitis. A third later developed acute hepatitis. A study of the injection habits suggested that the present low incidence of HAA and the decrease in number of cases with jaundice was probably related to the provision of free disposable syringes by the clinic since it was opened in 1968.


Neuraminidase and resistance to vaccination with live influenza A2 Hong Kong vaccines. Thirty-seven volunteers were inoculated intranasally with living attenuated influenza A2 viruses. Rising titres of circulating antineuraminidase (AN) were detected in 14 of 17 infected volunteers. AN was also found in nasal secretions. Statistical analysis showed that there was a correlation between the titres of haemagglutination-inhibiting antibody (HI) and AN in nasal washings, and between AN in blood and washings. Resistance to infection could be predicted from antibody titres in 29 of 37 volunteers and blood AN alone predicted the outcome of 25 volunteers.


The influence of pH of the culture medium on the sensitivity of Mycoplasma gallisepticum antigens for use in certain serological tests. Mycoplasma gallisepticum antigens were prepared from organisms cultured in broth medium with glucose. The influence of period of growth, pH of the medium and duration of incubation at low pH (5.0) on the sensitivity of these antigens was determined in certain tests. The most sensitive antigen for the serum plate test was harvested after no more than 8 hr. incubation at pH 5.0. Sensitivity in serum plate, haemagglutination and gel diffusion tests was impaired if organisms were incubated at pH 5.0 for long periods. Antigens prepared from buffered broth medium were found to be at least as sensitive as those from unbuffered medium for the haemagglutination and gel precipitation tests, but considerably less so for the serum plate test.


Aspects of influenza C virus replication. Influenza C virus antigen could be detected by immunofluorescence in chick amniotic epithelium 4 hr. after inoculation with undiluted amniotic fluid. Antigen could be detected in the nucleus of rhesus monkey kidney tissue cultures 10 hr. after infection. Only cytoplasmic fluorescence was observed 17-21 hr. after infection.The pattern of replication was similar to that reported for group I myxoviruses, indicating that the virus is correctly classified as an influenza virus.


Lung tissue and serum levels of methacycline.


Lead as an environmental poison.

Lead as an environmental poison.


The histochemical distribution and localization of copper, iron, neuromelanin and lysosomal enzyme activity in the brain of aging rhesus monkey and the dog.


The Rhesus syntenic group in man.

The Rhesus syntenic group in man.


Dermatophilosis in owl monkeys.

Dermatophilosis in owl monkeys.


The influence of haemolysis on the radioimmunoassay of insulin.


The influence of respiratory bacteria and their biochemical fractions on Aspergillus fumigatus.


Tissue-like proteins excreted in urine from transplanted kidneys (histuria).


Heterotransplantation.

Heterotransplantation.


Glomerular ultrastructure and macromolecular permeability following human renal transplantation.


Metabolism of radiolabelled C'3 and C'4 in human renal allograft recipients.


Clinical use of antilymphoblast serum.

Clinical use of antilymphoblast serum.


Symposium on bone marrow transplantation: experimental aspects in canines.


Chemistry of possible transplantation antigens.


The effect of lymph node permeability factor on renal allografts in rats.


Autoimmune concomitants of renal allografts.


Studies in immuno-suppression. Methods to evaluate anti-human lymphocyte sera.


Countercurrent distribution as a tool for purification of hypothalamic hormones on a preparative scale.


The role of folate coenzymes in the initiation of protein synthesis.


The evolution of placentation.

The evolution of placentation.


Studies on protein multimers. The association-dissociation behaviour of  -galactosidase in glycerol. 1. The effect of glycerol on the association-dissociation behaviour of beta-galactosidases from Escherichia coli is described. Two strains, K12 and ML308, were used as sources of enzyme. The conditions used, involving glycerol at a concentration of 90%, result in dissociation of the active 540000-dalton form to inactive structural subunits of 135000 daltons. 2. A pH-dependent process, assumed to be cyclic in mechanism, allows reassociation to an active form indistinguishable from the initial protein. 3. The apparently identical structural subunits, if produced in the presence of EDTA, were found to give rise to two electrophoretically distinguishable species. 4. Enzymes from both strains of E. coli can be distinguished electrophoretically but exhibit the same behaviour in glycerol. 5. A scheme of the association-dissociation is presented that is consistent with the behaviour observed and that has some predictive value.


The isolation, and amino acid and carbohydrate composition, of polymeric collagens prepared from various human tissues. 1. Insoluble polymeric collagens from various human tissues were prepared by the EDTA method. Almost all of the collagen from simple soft tissues such as dermis, tendon, submucosa, sclera and cornea could be extracted, whereas the more complex tissues such as intercostal cartilage and intervertebral disc yielded only small amounts of collagen. Amino acid and carbohydrate analysis indicated that most of the preparations were highly purified on the basis of their tyrosine, hexosamine, mannose, xylose and fucose contents. 2. Wide variation in the total hexose content was observed, the lowest being 8.5 residues/3000 amino acid residues for collagen from dermis and the highest being 42.1 residues/3000 in corneal collagen. The molar ratios of sugars also varied, submucosal collagen having a galactose/glucose ratio of 1.0 and corneal collagen having a ratio of 2.3. 3. The presence of glucosylgalactosylhydroxylysine was confirmed in submucosal collagen by compositional and chromatographic analysis of this component after its isolation from alkaline hydrolysates of the collagen. Evidence was also obtained for the presence of galactosylhydroxylysine. 4. Determination of the hydroxylysyl glycosides was carried out and it was observed that the amounts of these components varied widely from tissue to tissue. Corneal collagen contained 19.1 hydroxylysine-linked carbohydrate units/3000 amino acid residues, whereas tendon collagen contained only 4.1 units/3000. Variation in the ratio disaccharide unit/monosaccharide unit was also observed, the ratio being 1.2 in intercostal cartilage collagen and 4.1 in submucosal collagen. The proportion of the total hydroxylysine that was substituted by carbohydrate also varied from tissue to tissue.


Eli Lilly lecture. The subunits of pituitary thyrotropin--their relationship to other glycoprotein hormones.


New evidence on the old problems of trichloroethylene.


Studies on inherited antigenic variation of human serum -lipoprotein by passive hemagglutination.


Pathophysiologic responses to severe, chronic alloxan diabetes in arteriosclerotic and non-arteriosclerotic rats.


Proton translation in submitochondrial vesicles.


Control of mitochondrial swelling by mg2+. The relation of ion transport to structural changes.


Cation permeability induced by spermine and Polybrene in rat liver mitochondria.


Studies on the fluorescence of the human vitamin A-transporting plasma protein complex and its individual components.


Isolation and partial characterization of a human vitamin D-binding plasma protein.


On the regulation of a bacterial deoxycytidylate deaminase.


Phosphoenolpyruvate-dependent fructose phosphorylation in photosynthetic bacteria.


Possible participation of superoxide anion in the intestinal tryptophan 2,3-dioxygenase reaction.


Intestinal bacteria and the hydrolysis of glycosidic bonds.


The adjuvant effect of a tubercle bacillary lipid on the antibody response of rabbits to sheep red cells.


Bacteriological assessment of clindamycin, a new lincomycin derivative.


Alteration of the chemical composition of human immunoglobulin G by Streptococcus pyogenes.


Intracranial phycomycosis: a clinicopathological and radiological study.


A comparative study between the S--s--U--phenotype found in Central African Negroes and the S--s+U--penotype in Rhnull individuals.


Responses to influenza A 2  vaccines in man and vervet monkeys.


Symposium: the visually handicapped child. Introduction to the panel discussion.


CSF-specific proteins in perilymph.

CSF-specific proteins in perilymph.


Visual cue alternation and bodily equilibrium in squirrel monkeys.


Comparative conformational studies on the tryptic digestion fragments of human ummunoglobulins M and G.


Serum prostaglandin A isomerase.

Serum prostaglandin A isomerase.


Subunit structures of C-phycocyanin and C-phycoerythrin.


Intermediates in the biosynthesis of peroxisomal catalase in rat liver.


A study of some fast-growing scotochromogenic mycobacteria including species descriptions of Mycobacterium gilvum (new species) and Mycobacterium duv alii (new species).


Convulsive effects of 4-deoxypyridoxine and of bicuculline in photosensitive baboons (Papio papio) and in rhesus monkeys (Macaca mulatta).


Response properties and receptive fields of cells in an anatomically defined region of the superior temporal sulcus in the monkey.


Isolation of rabbit spleen ribonuclease with reverse immunosorbents.


Studies on human secretory IgA comparative studies of the IgA-bound secretory piece and the free secretory piece protein.


The human serum high density lipoprotein peptides of very low density lipoproteins and chylomicrons--an appendix.


Immunochemical heterogeneity of human high density serum lipoproteins.


Increased serum prolactin after administration of synthetic thyrotropin releasing hormone (TRH) in man.


Cleft lip and chorioideal coloboma associated with multiple hypothalamo-pituitary dysfunctions.


Prolonged and exaggerated elevations in plasma thyrotropin (HTSH) after thyroptropin releasing factor (TRF) in patients with pituitary tumors.


Biochemical and histopathological changes in the brains of mice inoculated with scrapie by the intraperitoneal route.


Blood copper and serum enzyme changes following copper calcium E.D.T.A. administration to hill sheep of low copper status.


Louping-ill encephalomyelitis in the sheep. 3. Immunoglobulins in cerebrospinal fluid.


Synovitis induced by killed Erysipelothrix rhusiopathiae. Extended reaction in passively immunized lambs.


The metabolic pathway in the degradation of glyceryl trinitrate.


Superprecipitation of actomyosin extracted from crayfish skeletal muscle: the effect of heavy metal cations, EDTA and drugs.


Postictal pulmonary edema. Report of a case.


Immune factors in human cancer: malignant melanomas, skeletal and soft tissue sarcomas.


Incontinentia pigmenti associated with raised alkaline phosphatase and disturbance of the plasma proteins.


Carbonic anhydrase interaction with DDT, DDE, and dieldrin. The active site of various carbonic anhydrases is not blocked by DDT. DDE, or dieldrin. Impairment of catalytic efficiency can only be documented in opalescent test solutions in which the insecticides are present in excess of their solubility limit. These slowly forming precipitates occlude enzyme from solution and furnish a physical explanation of the supposed inhibition. The amounts occluded depend, among other things, on the conformational and topographical characteristics of the particular carbonic anhydrase molecules.


Difficulty of evaluating the toxicity and teratogenicity of 2,4,5-T from existing animal experiments.


Cerebral blood flow during increased subarachnoid pressure. The influence of systemic arterial pressure.


The biosynthesis of terpenoid quinones.

The biosynthesis of terpenoid quinones.


Rat liver proteins in experimental dysproteinism.


Inhibition by chloramphenicol of aminoazo dye carcinogenesis in rat liver: studies of biochemical changes in rat liver and protein binding of carcinogen.


The differential diagnosis of large bowel strictures.


Craniostenosis.

Craniostenosis.


Techniques in megavoltage radiotherapy: no. 3, carcinoma of the rectum.


Effect of dietary fat and cholesterol on circulating lipids and ortic ultrastructure of squirrel monkeys.


Subacute sclerosing panencephalitis (SSPE) agent in hamsters. I. Acute giant cell encephalitis in newborn animals.


The source of the inter-alpha trypsin inhibitor in pathologic hyaluronateprotein.


Suppression of tryptophan mutants in Bacillus subtilis by apparent nonsense suppressors.


Localization of tRNA genes in the salivary chromosomes of Drosophila by RNA:DNA hybridization.


In vitro effect of various hypoglycemic agents on the hepatic and pancreatic protein synthesis in the rat.


Studies on gene conversion and its relationship to linked exchange in Drosophila melanogaster.


Styles of planning health services: the United states, Sweden, and England.


Flash photolysis of flavins. V. Oxidation and disproportionation of flavin radicals.


Affinity of human brain acetylcholinesterase to some organophosphates and carbamates in vitro.


Existence of a breaking point in cilia and flagella.


Disodium cromoglycate (INTAL).

Disodium cromoglycate (INTAL).


Contraction of single smooth muscle cells from Bufo marinus stomach.


Oxygen dissociation curves of fetal and adult baboons.


The metabolism of progesterone in the rhesus monkey (Macaca mulatta).


Immunological identity of the multiple forms of beef liver mitochondrial monoamine oxidase.


Comparison of mitochondrial monoamine oxidases from bovine brain and liver using antibody to purified liver monoamine oxidase.


Studies on Rh prophylaxis. 1. Relationship between doses of anti-Rh and size of antigenic stimulus.


Catecholamine fluorescnece in primate digital arteries.


The influence of delay interval on severity of the spatial alternation deficit in frontal monkeys.


Emotion, consciousness and will after brain bisection in man.


Studies on Rh prophylaxis. II. Rh immune prophylaxis after transfusion with Rh-positive blood.


Red blood cell sensitization due to unexpected Rh antibodies in immune serum globulin.


National health planning--an appraisal of the state of the art.


Serum protein polymorphisms in Indians of western Canada. Gene frequencies and data on the Gc-albumin linkage.


Contribution of health to development.

Contribution of health to development.


Inhibition of growth and RNA biosynthesis of Bacillus cereus by quinacrine.


A viability assay for Tetrahymena pyriformis.


A turbidimetric method for the assay of pyocin activity.


Uniformity of thylakoid structure in a red, a brown, and two blue-green algae.


Structure of the  -chain of chimpanzee haemoglobin A 2 .


Transformation of lymphocytes from monkeys with experimental allergic encephalomyelitis.


Selective effect of salicylate on microbial RNA biosynthesis.


Tissue reactions and sealing properties of Tubulitec and Dropsin.


Collagen metabolism in periodontal membrane of the marmoset, influence of periodontal disease.


Structure and incidence of Gudden's tegmental nuclei in some mammals.


Physical characterization of DNA from bacteriophage SP 3 .


The combined action of pyrimidines and sulfonamides or sulfones in the chemotherapy of malaria and other protozoal infections.


Studies on the small subunit of rat liver ribosomes: some biochemical properties with specific references to the reconstruction of the small subunit.


Selective blockade of the vasodepressor response to prostaglandin F 2   in the anaesthetized rabbit. 1. The prostaglandin-blocking activity of meclofenamic acid, N-(2,6-dichloro-m-tolyl)anthranilic acid (CI-583), was analysed in the anaesthetized rabbit. PGF(2alpha), PGE(1) and isoprenaline were injected before and after meclofenamic acid infusion.2. Isoprenaline produced a fall in blood pressure, a reduction in oviduct motility and a reduction in uterine motility if the uterus showed marked spontaneous motility. PGF(2alpha) uniformly produced a fall in blood pressure and an increase in both uterine and oviduct contractility. PGE(1) produced a fall in blood pressure, a reduction in oviduct motility and no consistent effect on uterine motility.3. Meclofenamic acid selectively blocked the vasodepressor response to PGF(2alpha). The vasodepressor responses to PGE(1) and isoprenaline as well as the effects of all three agonists on uterine and oviduct contractility were not reduced by treatment with meclofenamic acid.4. Polyphloretin phosphate (PPP) administered to two rabbits in a cumulative dose of 94 mg/kg showed no significant blocking action on the vasodepressor or uterine and oviduct contractor responses to PGE(1) or PGF(2alpha) though this compound, like meclofenamic acid, has been reported to antagonize the actions of PGE(1) and PGF(2alpha) on isolated smooth muscle preparations.


Comparison of human serum, parotid and mixed saliva levels of phenoxymethylpenicillin, ampicillin, cloxacillin and cephalexin. 1. A study has been made of serum, mixed and parotid salivary levels attained in normal volunteers following oral dosage of 500 mg phenoxymethylpenicillin tablets, 500 mg crushed phenoxymethylpenicillin tablets in capsules, 500 mg ampicillin, 500 mg cloxacillin and 500 mg cephalexin.2. High mixed saliva levels were obtained with phenoxymethylpenicillin tablets and it is considered that these were due to rapid intra-oral dissolution of surface powder from friable tablets. No saliva levels were detected when tablets from the same batch were put into capsules.3. Low or no saliva levels were achieved with ampicillin, cloxacillin and cephalexin.4. The mode of action of antibiotics in oral infections is discussed.