Two forms of yeast glycogen synthetase and their role in glycogen accumulation. The glycogen content of yeast rises dramatically just before the onset of the stationary phase of growth. Concomitantly, a rapid increase was found in the glucose 6-phosphate-independent (I) activity of glycogen synthetase, as well as in the total amount of enzyme. A mutant (GS 1-36) was obtained, which did not accumulate glycogen during growth. The synthetase from this strain was in the glucose 6-phosphate-dependent (D) form at all times. The total enzymatic activity of the mutant also increased sharply at the end of the logarithmic phase, although its maximal value was only one third that of the parent strain. Incubation with glucose of wild type resting cells from the logarithmic phase resulted in a slow accumulation of glycogen, which was accelerated after 20 min. At the same time a transformation from the D to I form of the enzyme was detected. The same slow initial rate of glycogen deposition was found with stationary cells of mutant GS 1-36, but the rate gradually declined to zero, rather than accelerating. The interconversion of the I and D forms was obtained with extracts from cells harvested during different phases of growth. Examination of the properties of the I and D forms showed that the latter was much more strongly inhibited by ATP at low glucose 6-phosphate concentration. These findings clearly establish the fundamental role of the I form in glycogen accumulation. When taken together with previous results, they also show that the physiological significance of the I-D interconversion depends on the concentration of glucose 6-phosphate. Under certain conditions glucsoe 6-phosphate appears to regulate directly the activity of the predominant form of the enzyme.


Demonstration of I-anti-I interaction in a precipitin system.


Effect of ethylenediamine tetraacetate on the germicidal action of disinfectants against "Pseudomonas aeruginosa".


Tactile discrimination performance in the monkey: the effect of unilateral posterior parietal discharging lesions.


Tactile discrimination performance in the monkey: the effect of unilateral posterior parietal ablations.


Jejunal diverticulosis with malabsorption leading to osteomalacia and probable rickets.


Cyclandelate in long standing cerebral arteriosclerosis.


Effects of hypophysectomy on RNA metabolism in rat brain stem.


Relation of three enzymes of transsulphuration to the concentration of cystathionine in various regions of monkey brain.


Congenital diverticulum of the bladder.

Congenital diverticulum of the bladder.


Remission of metastatic lesions following cryosurgery in prostatic cancer: immunologic considerations.


Bacteria, viruses and mycoplasmas in the lower urinary tract of a group of renal transplant recipients.


Direction of ataxic gait after unilateral partial destruction of the vestibular system in squirrel monkeys.


Serum hepatitis antigen (SH): rapid detection by high voltage immunoelectroosmophoresis. An immunoelectroosmophoretic technique for rapid detection of the antigen (SH) associated with the serum hepatitis virus has been devised. The technique maintains the specificity characteristic of the Ouchterlony gel-diffusion method, yet detects in 1 to 2 hours one-tenth the amount of antigen required for gel diffusion. The test has immediate application to blood-banking practice since it permits the screening of such labile products as platelets and fresh whole blood, and the detection of antigen in additional serums negative by the Ouchterlony technique.


Junctional membrane permeability: restoration by repolarizing current. In cells of the Chironomus salivary gland, junctional membrane conductance, depressed by various chemical treatments, is restored to its normal high level by currents passed inward through nonjunctional cell membrane.


Chemical basis of hashish activity.

Chemical basis of hashish activity. A sample of hashish was extracted consecutively with petroleum ether, benzene, and methanol. When tested intravenously in monkeys only the petroleum-ether fraction was active. This material was further fractionated. The only active compound isolated was Delta(1)-tetrahydrocannabinol. Cannabinol, cannabidiol, cannabichromene, cannabigerol, and cannabicyclol when administered together with Delta(1)-tetrahydrocannabinol do not cause a change in the activity of the latter, under the experimental conditions used. These results provide evidence that, except for Delta(1)-tetrahydrocannabinol, no other major, psychotomimetically active compounds are present in hashish.


Complement components in synovial fluid: activation and fixation in seropositive rheumatoid arthritis.


Distribution of rheumatoid factor activity in nonrheumatoid states.


GammaM rheumatoid factors reacting with nitrophenyl groups and denatured deoxyribonucleic acid.


Severe bradycardia induced by caloric test in cardiac patients.


Relationship of Cx-reactive protein response to induction of liver and kidney catalases following hematin administration into rabbits.


Mutations and loss of transforming activity of Bacillus subtilis DNA after reaction with esters of carcinogenic N-hydroxy aromatic amides.


The absorption, distribution, excretion, and biotransformation of the carcinostatic 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea in animals.


Multiple somatic projections to frontal lobe of the squirrel monkey.


Action of w-chloroacetophenon on several enzymes.


Fortuitous scan documentation of the development of an intracerebral hematoma. Case report.


Disappearance of anti-D antibody without reappearance during a subsequent pregnancy at risk.


Control of the hypermagnesaemia of renal failure by maintenance haemodialysis.


Frequency of blood groups of the ABC and Rho(D) system in patients with viral hepatitis, cirrhosis of the liver, bile stones, pancreatitis, urolithiasis and ulcerative colitis.


Leucocyte groups in baboons tested with human antisera.


Contracture of proximal interphalangeal joints of the fingers associated with pes valgus.


Serological identification of mycoplasma isolates by agar gel double-diffusion technique.


Effect of low level X radiation on the antigenicity of Escherichia coli.


An immune factor in baboon anti-candida serum.


Studies on the biology of the periodontium of marmosets. IX. Effect of parathyroid hormone on the alveolar bone of marmosets pretreated with fluoridated and nonfluoridated drinking water.


Primate experiments on facial asymmetry.


Effect of anti-lymphocytic serum on experimental infection with Candida albicans in guinea pigs.


Evaluation of group C meningococcal polysaccharide vaccine in marine recruits, San Diego, California.


Foamy virus infection of rhesus and green monkeys in captivity.


Fantastic reality.

Fantastic reality.


Avian influenza antibody detection by immunodiffusion.


Detection of Marek's disease herpesvirus antigen by fluorescent and coating antibody.


The effect of proteolytic enzymes on acetylcholinesterase activity, the sodium pump and choline transport in human erythrocytes.


Complexing of transcobalamin 2 and apparent combination with heparin.


Genetic properties of a membrane-bound fraction of DNA in the culture of Bacillus subtilis with synchronously replicating chromosomes.


Bacterial metabolism of thiamine. 3. Metabolism of thiamine to 3-(2'-methyl-4'-amino-5'-pyrimidylmethyl)-4-methyl-thiazole-5-acetic acid (thiamine acetic acid) by a flavoprotein isolated from a soil microorganism.


Electron microscopic study of tissue cultures infected with simian haemorrhagic fever virus.


Hepatic sinusoidal lymphocytosis and malaria in rhesus monkeys.


Blood grouping in Somalia.

Blood grouping in Somalia.


Listeria monocytogenes.

Listeria monocytogenes.


Socio-economic factors in nursing.

Socio-economic factors in nursing.


The dollar crisis in health.

The dollar crisis in health.


Clinical instruction in marine hospitals in the 19th century.


Motivational and behavioral effects of modifying health beliefs.


Physicians in family practice 1931-67.

Physicians in family practice 1931-67.


Ethnic differences in psychiatric symptoms reported in community surveys.


Jarvis' law and the planning of mental health services. Influence of accessibility, poverty, and urbanization on first admissions to Connecticut State hospitals.


Emergency health services for the nation.


Jarvis' law and the planning of mental health services. Role of alternative psychiatric service in Connecticut.


Demographic factors in opiate addiction among Mexican-Americans.


Primary isolation of Neisseria gonorrhoeae on hemoglobin-free Thayer-Martin medium.


Involvement of alcohol in fatalities of Wisconsin drivers.


Occupational health services for Federal employees.


Team learning in community medicine for medical and paramedical students.


Phanerozoic stromatolites: noncompetitive ecologic restriction by grazing and burrowing animals. The abundance of stromatolites (algal laminated sedimentary structures) in the Precambrian followed by a decline in the Phanerozoic is explained by the evolution and diversification during the Phanerozoic of grazing animals which feed on surface algal mats and of burrowing animals which destroy sedimentary laminations.


Bacterial flagella: polarity of elongation. Newly synthesized regions of Bacillus subtilis flagella were labeled with fluorophenylalanine or [(3)H]leucine. The flagella were then examined for altered gross morphology or by radioautography. Results of both experiments indicate that flagella elongate in vivo by polymerization of flagellin subunits onto the distal end of the filament.


Effect of O-carbamyl-D-serine on the growth of mycobacterium tuberculosis.


Primary drug-resistant tuberculosis in children. A continuing study of the incidence of disease caused by primarily drug-resistant organisms in children observed between the years 1965 and 1968 at the Kings County Medical Center of Brooklyn.


[Clinical reliability of stabilized and nonstabilized tuberculin PPD].


Capacity of tubercle bacilli for isoniazid accumulation.


Selective deficiency of IgA immunoglobulins associated with carcinoma of the stomach.


The effect of transferrin-free serum on the utilization of iron by rabbit reticulocytes.


The stability of the x-ray beam from an 8 MV linear accelerator designed for radiotherapy.


Diverticulum formation after oblique end-to-end anastomosis for jejuno-ileal atresia.


Respiratory symptoms and washing powders.


Familial thyroxine-binding globulin deficiency. Genetically determined thyroxine-binding globulin deficiency is described in two families in the United Kingdom. All subjects in both pedigrees were euthyroid. Transmission was by sex-linkage; males showed low serum protein bound iodine and high thyroxine (T4) resin uptake due to complete absence of serum thryroxine-binding globulin; females were less severely affected. The distinctive biochemical results disclosed the diagnosis and emphasize that serum protein bound iodine levels should be interpreted carefully and tests, such as triiodothyroinine (T3) or T4 resin uptake, used to prevent erroneous diagnoses of hypothyroidism.


Diabetic ketosis. Serial plasma growth hormone concentrations during therapy.


Catecholamines, disseminated intravascular coagulation and shock.


The rising cost of health care.

The rising cost of health care.


Some practical aspects of chronic respiratory diseases in children.


Drugs, drunken driving, the delivery of medical care, the high cost of hospitalization.


Behaviour of Hela cells grown on young chick blastoderms in vitro.


The cerebral inhibition of gastric acid.


Forward facing versus rearward facing passenger seats during emergency descent of multi mach-high altitude transport aircraft.


Multistage electrocardiographic exercise tests. Principles and clinical applications.


Preservation of the primate liver by simple cooling and orthoptic autotransplantation for 2 and one-half-6 hours.


Relation between cerebral blood-flow and cerebral perfusion pressure.


Studies in calcitonin M and D.

Studies in calcitonin M and D.


A morphogenetic matrix for differentiation of bone tissue.


The activity of carbenicillin against gram negative rods compared with that of penicillin, ampicillin and cephalosporins.


On some changes of blood proteins in patients with resection of the stomach according to Billroth II.


Changes in the natural beta radioactivity of the atmospheric aerosols in Plovdiv in connection with some meteorologic factors.


On the yearly course of radon and thoron inductions and the long lasting radioactivity of aerosols in Plovdiv.


Regenerative possibilities of endometrium after interruption of pregnancy according to vacuum-excochleation method. Regenerative possibilities of the endometrium after interruption of pregnancy by the vacuum-excochleation method were studied in 50 women between the ages of 20 and 33.  Cases of partial early regeneration were found on Day 4 postabortion while a complete regeneration was found on Day 6 in some cases.  A transition picture toward a secretory phase was seen on Days 16 and 17.  This early regeneration may be due to the preservation of the basal layer of the mucous membrane during the procedure.  Following the abortion, all the women menstruated on time except 4 who did so 6 days late.


Comparison of inulin and chromium-EDTA spaces in the nephrectomized baboon.


Phenolase inhibitor from cultures of Penicillium expansum which may play a part in fruit rotting.


Immunity to Plasmodium falciparum in owl monkeys (Aotus trivirgatus).


Effects of vertical forces on the mandible and total face height.


Long-term course in 119 cases of pseudarthrosis of the medial malleolus.


Tissue changes in the monkey following root torque with the Begg technique.


Apical basilar membrane herniations and hydrops.


The diagnosis and treatment of esophageal cancer.


Tolerance induction by an apparently non-immunogenic molecule.


Separation of parasites in sucrose gradients.


Cutaneous clues to visceral cancer.

Cutaneous clues to visceral cancer.


Unproven methods of cancer treatment.

Unproven methods of cancer treatment.


Man and animals not identical.

Man and animals not identical.


Gonococci in urethral exudates possess a virulence factor lost on subculture.


Cell septation during synchronous growth of Bacillus subtilis.


Elective radical neck dissection: an assessment of its use in the management of papillary thyroid cancer.


Long-term parenteral nutrition through an external arteriovenous shunt.


Management of invasive cervical cancer following inadvertent simple hysterectomy.


Etiology of tubal ectopic pregnancy. Radiologic and pathologic studies.


Gross anatomy of the placenta of rheusus monkeys.


The interface between dentine and irregular secondary dentine.


The coagulation mechanism in acute bacterial infection.


Exchange between parenteral and tissue folate in man.


Experimental lead poisoning in the baboon.


Pathogenesis of visual field defects. Role of the ciliary circulation.


Experimental tests of corrections for tissue inhomogeneities in radiotherapy.


Diagnosis of vaginal discharge.

Diagnosis of vaginal discharge.


Immunological and immunochemical investigations of patients suffering from general paralysis.


Antibiotic sensitivity of gonococci and treatment of gonorrhoea in Uganda.


Sensitivity of gonococci to antibiotics in strains isolated from "prostitutes" in Copenhagen.


House-dust mite asthma. Results of challenge tests on five criteria with Dermatophagoides pteronyssinus. Twenty-eight patients with asthma and house-dust allergy were investigated by five different challenge tests with mite extract (Dermatophagoides pteronyssinus). These tests were performed on the skin, bronchi, nasal mucosa, and leucocytes, and on normal human lung tissue passively sensitized by the patients' serum. The results were compared with each other and with the clinical state of the patients.Of 21 patients on whom all five tests were carried out, positive reactions to every test were found in 14 (67%). Asthmatic attacks were readily induced by inhalation of the mite extract and occurred in 18 (86%). Nasal reactions were provoked in 18 (86%) and were not followed by asthmatic symptoms. A leucocyte test was positive in 19 (90%) and the serum test was positive in all (100%). Delayed asthmatic responses to bronchial challenge were seen in six patients, but there was no evidence that these were mediated by precipitins. Quantitatively there was no fixed pattern between the degrees of sensitivity of the different tissues in the same patient. The amount of mite antigen capable of causing an asthmatic reaction was extremely small-less than 1 mug. of mite material in several patients.In view of the prevalence of D. pteronyssinus in this country and its high allergenic potency, it is considered that this mite is an important factor in allergic asthma.


Free thyroxine index.

Free thyroxine index.


Typing the gonococcus.

Typing the gonococcus.


Investigation of the normal TBG levels found in thyroid disease.


Influence of dietary sucrose on glucose and fructose tolerance and triglyceride synthesis in the baboon.


Comparative studies on mitochondrial and soluble diamine oxidases.


Hereditary deficiency of the second component of complement (C2) in man: correlation of C2 haemolytic activity with immunochemical measurements of C2 protein.


Household pets and cancer.

Household pets and cancer.


Total body water of the pig-tailed monkey, Macaca nemestrina.


Unproven methods of cancer treatment.

Unproven methods of cancer treatment.


Induction and timing of single and multiple ovulations in the squirrel monkey (Saimiri sciureus).


Reproductive cycles of two Cercopithecus monkeys.


Buckels LJ, De Meester TR,+DEMEESTER TR, MacDonald W,+MACDONALD W: Gonococcal endocarditis with severe aortic regurgitation: early valve replacement.


Therapeutic innovation: ethical boundaries in the initial clinical trials of new drugs and surgical procedures.


Spectrophotometric analysis of amniotic fluid in anencephalic pregnancies.


The significance of rhesus antibodies in liquor amnii.


Virulence of trachoma-inclusion conjunctivitis agent after passage in baboons.


Survey of workers exposed to dusts containing derivatives of Bacillus subtilis. In a survey of 121 workers exposed to dusts containing derivatives of Bacillus subtilis, mainly proteolytic enzymes, skin tests showed evidence of sentiztation was higher among "atopic" subjects-16 out of tization was higher among "atopic" subjects-16 out of 25 (64%)-than among "non-atopic" subjects-32 out of 96 (33%). Reduced ventilatory capacity was found in 44% of sensitized workers compared with 14% of those not sensitized.


Systematization, completion and differentiation of enzymic inhibition types.


The syndromes of the ossified stylohyoid chain.


Suggested management of prematurely ruptured membranes.


American trypanosomiasis. An historical and a human lesson.


Effect of2, 2'-(9,10-anthrylenedimethylene)bis(2-thiopseudourea) on nucleic acid synthesis in bacteria.


Kinetics of carbonic anhydrase in whole red cells as measured by transfer of carbon dioxide and ammonia.


Fluorides and human health. Introduction.


Excretion of fluorides.

Excretion of fluorides.


The supply of fluorine to man.

The supply of fluorine to man.


Physiological effects of small doses of fluoride.


Toxic effects of larger doses of fluoride.


Absorption of fluorides.

Absorption of fluorides.


Distribution of fluorides.

Distribution of fluorides.


Immunologic aspects of renal disease.

Immunologic aspects of renal disease.


Behaviorally induced hypertension in the squirrel monkey.


Urinary excretion of folic acid activity in man.


A satellite unit.

A satellite unit.


The control of a microsporidian, Nosema sp., in an anopheline colony by an egg-rinsing technique.


Guinea pig serum and liver L-asparaginases. Comparison of serum and papain-digested liver L-asparaginases.


Diverticulosis of the appendix.

Diverticulosis of the appendix.


Cooperative conformational transitions of linear biopolymers.


An unusual structure in Kuru brain.

An unusual structure in Kuru brain.


A general synthesis of pyrimidine nucleosides.


Separation of nucleosides on cytidine-containing polymer gels in DMSO-CHC13 or water.


Growth characteristics of selected mutants of a coccoid blue-green alga.


Autoantibodies and the hepatitis-associated antigen in acute infective hepatitis. In 39 unselected patients with acute infective hepatitis diagnosed on clinical, epidemiological, and pathological findings, smooth muscle (S.M.) antibody was found in 87% and hepatitis-associated antigen in 33%. The incidence of S.M. antibody was highest during the first month after the onset of symptoms, and the antibody was preponderantly IgM at this stage.S.M. antibody and hepatitis-associated antigen occurred independently, and unlike hepatitis-associated antigen, which was strongly associated with possible parenteral exposure to infection, the incidence of S.M. antibody was not related to the presumed mode of exposure. It is concluded that production of S.M. antibody is more directly related to liver cell damage than to the presence of hepatitis-associated antigen in the serum.


Salivary amylase. II. Alpha-amylase in salivary glands of the Macaca irus monkey, the Cercopithecus aethiops monkey, and man.


Australia antigen and autoantibodies in chronic hepatitis. The sera of 110 patients with chronic hepatitis and adequate controls were examined for antibodies to smooth muscle (S.M.), mitochondria (M.), and for antinuclear factors by the immunofluorescence method, and for Australia (Au(1)) antigen by a modified micro-Ouchterlony immunodiffusion technique. Twelve out of 13 patients with primary biliary cirrhosis had M. antibodies, two had antinuclear factor, and none had Au(1) in their sera. In chronic aggressive hepatitis 23.5% of the sera contained antinuclear factor, 13% S.M. antibodies, 10.5% M. antibodies, and 22% Au(1) antigen. Of the 12 patients with chronic persistent hepatitis, one had antinuclear factor, one S.M. antibodies, and three Au(1) antigen.The most striking finding was a mutual exclusion between Au(1) antigen and M. and S.M. antibodies. None of the 33 patients with one or the other form of chronic hepatitis and M. or S.M. antibodies had Au(1) antigen; 22 out of 77 (28%) patients without such antibodies were positive.


Enzyme therapy in experimentally produced atherosclerosis.


Some observations on the treatment of porphyrias with CaEDTA.


Viral isolation studies of inclusion positive biopsy from human connective tissue diseases.


The baboon (Papio ursinus) heart (coronary blood supply, muscle function and metabolism).


Transferrins of Cercopithecinae.

Transferrins of Cercopithecinae.


The significance of lysozyme (muramidase) in rheumatoid arthritis. I. Levels in serum and synovial fluid.


The hemodynamics of the pig-tailed monkey (Macaca nemestrina).


Urate oxidase in primates.

Urate oxidase in primates.


Quantitative studies on scent marking in the marmoset (Callithrix jacchus).


Gene expression after transformation of Bacillus subtilis. Sensitive assays of histidase activity were used to follow the production of this enzyme as directed by a gene newly introduced into cells of Bacillus subtilis by transformation. Histidase activity can be detected in histidase-negative recipient cells within 1 hr after the addition of deoxyribonucleic acid extracted from histidase-positive donors. Enzyme production continues for one to two additional hours and then ceases. Histidase production in the transformed cells is fully sensitive to catabolite repression. Catabolite repression is rapidly established after transformation of recipient cells that are resistant to this form of regulation.


Chemical composition of the cell wall of the H37Ra strain of Mycobacterium tuberculosis. The cell wall of the H37Ra strain of Mycobacterium tuberculosis was isolated and freed of extraneous noncovalently linked material by a series of extraction and enzymatic procedures. Chemical analysis of the cell wall has revealed the following composition: 22.8% amino acids, principally alanine, glutamate, and diaminopimelate in a molar ratio of 1:1.8:0.8; 24.7% reducing sugars, all in the form of arabinose and galactose in a molar ratio of 2.6:1; and 3.95% amino sugars, all in the form of glucosamine, muramic acid, and galactosamine in a molar ratio of 1:6.6:0.8. About 32.1% of the dry weight of the cell wall is lipid, of this about 55% is in the form of two series of mycolic acids. Each series of mycolic acids contains two homologues differing by 28 mass units. One pair of homologues contains in each a carbonyl function and an unsaturated double bond; the other pair contains two cyclopropane groups in each homologue. The remaining lipids are composed principally of normal saturated fatty acids, including tuberculostearic acid.


Composition and properties of a group A streptococcal teichoic acid. Teichoic acid-like material extracted by cold trichloroacetic acid from lyophilized whole cells of streptococci from groups A,D,E,O, and T was shown to give a positive precipitin reaction with group antisera. Similar material from cells of groups B,C,F,G,H,K,L,M,N,P,Q,R, and S did not give a positive reaction with group antisera. The group A material also reacted with anti-E serum; however, the opposite did not occur. A similar result was also obtained on the group T material and anti-O serum. The group A teichoic acid was purified by Sephadex column chromatography, and was shown to be free of cell wall peptidoglycan and polysaccharide, and ribitol teichoic acid. It was composed of glycerol, phosphate, alanine, and glucosamine. Alkaline hydrolysis showed the presence of ester-linked alanine and glucosaminylglycerol. Phosphorus was released from ester linkage by alkaline phosphatase. N-acetylglucosamine produced a 72% inhibition of the precipitin test at a level of 10 mumoles, and d-alanine methyl ester was significantly stronger than the l-alanine ester. A single precipitin band was seen with group A serum. The data indicate that teichoic acid of group A streptococci is a polymer composed of glycerol phosphate and containing N-acetylglucosamine and alanine. Antisera to these streptococci contain antibodies specific for the alanine and the glucosamine linkages. The use of serum containing antibodies to alanine-polyglycerophosphate shows that the occurrence of this type of teichoic acid is widespread among the streptococci.


Repair of alkylation damage: stability of methyl groups in Bacillus subtilis treated with methyl methanesulfonate. Bacillus subtilis was not inactivated and was able to replicate even though approximately 3 x 10(4) methyl groups added by methyl methanesulfonate (MMS) were bound to the deoxyribonucleic acid (DNA) of each organism. No significant loss of methyl groups from the DNA occurred for several generations upon incubation of methylated wild-type or MMS-sensitive cells. Single-strand breaks were not observed in the DNA from cells treated at this low MMS dose. Higher doses of MMS resulted in significant killing of both wild-type and MMS-sensitive strains, and the DNA extracted from such treated cells sedimented more slowly than control DNA through alkaline sucrose gradients, indicating the presence of breaks or apurinic sites (or both). These breaks were repaired upon incubation of wild-type but not of MMS-sensitive strains. Repair of damage induced by alkylating agents is probably the repair of breaks which occur as a consequence of high levels of alkylation.


Intercellular effects on development of competence in Bacillus subtilis. The intercellular transfer of competence during growth under the conditions specified by the transformation procedure of Spizizen was investigated with Bacillus subtilis 168. The rate of competence development as assayed uniformly in medium B was not affected by variations in the cell concentration, although the first appearance of transformants occurred earlier with high cell densities in medium A, approximately in proportion to the onset of the stationary phase in the culture. Growth in the presence of Pronase enhanced the frequency of transformation, but did not detectably alter the kinetics of competence development. The rate of competence increase in physiologically noncompetent cultures was not changed by mixing with competent cultures either in medium A or in medium B; however, an early appearance of transformants was noted in mixed cultures in which the proportion of competent to noncompetent cells prevented exponential growth of the noncompetent strain. These experiments indicate that the normal development of competence in B. subtilis is not mediated by a soluble or loosely bound protein factor capable of transmitting competence directly via cell contact. The onset of competence is thus a function of internal physiological changes which are induced by the overall metabolic state of the culture.


Characteristics of a stable, filamentous mutant of a coccoid blue-green alga. Filamentous mutants were induced in a coccoid blue-green alga, Agmenellum quadruplicatum strain BG1, after treatment with N-methyl-N'-nitro-N-nitrosoguanidine (NTG). The mutants fall into two general classes: filaments with cross walls and filaments without cross walls. All mutants of these general types derived from BG1 are stable and have growth rates the same as or very similar to the wild type under a variety of conditions. Detailed examination of one mutant, 53SB2, revealed no difference in deoxyribonucleic acid content nor in base ratios. Mutant 53SB2 did not revert to the normal cell size and shape when grown under different physical conditions nor upon the addition of potential reversing agents to the basal medium. It is our general experience that filamentous mutants such as those described here in BG1 are commonly induced in other coccoid blue-green algae after NTG treatment.


Effect of miracil D on marker frequency ratio and cytotoxicity in Bacillus subtilis. When Miracil D was added to mid-log phase Bacillus subtilis cells, the rate of growth decreased immediately, and the turbidity of the culture began to decrease within 15 to 20 min after addition of the drug. At this stage, ghostlike cells were observed under phase-contrast microscopy. The viable count also began to decrease rapidly after 15 min in the presence of the drug, and within 60 min there was a 10,000-fold reduction in viability. Incorporation of (3)H-thymidine into deoxyribonucleic acid (DNA) proceeded normally up to 20 min of exposure to the drug, after which incorporation ceased. Cells which were prelabeled with (3)H-thymidine and exposed to the drug released labeled DNA into the medium after 15 to 20 min. Results of transformation analyses with donor DNA from cells grown in the presence of Miracil show a decrease with time in the ratio of origin markers to terminus markers. Electron micrographs of sectioned cells grown for 5, 10, and 15 min in the presence of Miracil D show profound cytotoxic effects. The most striking aspect of such cells is the very condensed appearance of the nucleoid. In those cells about to divide, the nucleoid appears not to be properly separating, being strung out in the region of the developing septum. Finally, the mesosome is very poorly defined or entirely absent in Miracil-treated cells.


Isolation of L-forms of Bacillus subtilis which grow in liquid medium. L-forms of Bacillus subtilis can be isolated by treatment of the parent strain sequentially with N-methyl-N'-nitro-N-nitrosoguanidine and lysozyme and selection of the surviving protoplasts on semisolid medium containing 2,000 units of penicillin per ml. Some of these clones can be adapted to grow in liquid cultures containing 1.2 m NaCl. This method will aid in the isolation of cell wall mutants which require hypertonic medium for growth.


Correlation between pigment production and amino acid requirements in Bacillus subtilis. Several Bacillus subtilis W-23 auxotrophs were unable to produce wild-type pigment normally on minimal agars supplemented sufficiently for growth. This offers a reliable means for scoring genotypes.


The passive haemagglutination test for th detection of Mycoplasma suipneumoniae and the possible diagnosis of enzootic pneumonia of pigs. Fourteen cases of enzootic pneumonia, nearly all of which had presented diagnostic difficulties using the metabolic-inhibition test, were re-examined using specific pig antisera in the passive haemagglutination test (PHA). All proved positive for Mycoplasma suipneumoniae, indicating that the test, used in this manner, might be particularly valuable for routine diagnosis.The PHA test was also used to demonstrate antibody to M. suipneumoniae in pneumonic tissue and the associated bronchial lymph nodes.To allay our concern that cross-reactions might interfere with this and other serological tests-the complement-fixation test (CF) and precipitation in agar-gel-the specificity of our reagents and the antigenic relationships of Mycoplasma hyorhinis, Mycoplasma granularum, mycoplasma B3, Mycoplasma hyopneumoniae and three strains of M. suipneumoniae (including cloned and uncloned isolates of the J strain) were studied in various ways. Antibodies to medium constituents occurred in rabbit antiserum but did not present a problem with pig antisera. These antibodies were successfully absorbed from the rabbit antisera but it was not possible to remove medium constituents from the antigens used to produce antisera in rabbits by repeated washing.By all these tests, the main species of mycoplasmas studied seemed to be antigenically distinct. No major antigenic differences between the three strains of M. suipneumoniae were revealed by the PHA test and the CF test; a slight difference in the precipitation lines of one of these strains (MG) in agar-gel might have indicated an antigenic variation or been a measure of some other factor.


Inactivated smallpox vaccine. A comparison of inactivation methods. Vaccines were prepared from a single pool of high-titred vaccinia virus and inactivated by six methods, namely heat, formalin, hydroxylamine, beta-propiolactone, ultraviolet irradiation, and visible light and methylene blue. Large doses of the vaccines were required to protect mice against intracerebral challenge. Differences in protection were not attributable to the method of their inactivation. The vaccines also induced similar degrees of skin immunity in rabbits which showed no severe dermal reactions when challenged with either homologous killed vaccine or live virus. The virus-neutralizing, haemagglutinin-inhibiting and complement fixing antibody responses to the vaccines differed; heat-inactivation preserved these antigens least well and beta-propiolactone apparently the best. In both rabbits and mice there was little association between the different antibody responses to each vaccine or between the degrees of antibody response and the protection they induced. The relation of these findings to pox-virus immunity and the use of inactivated smallpox vaccine in man is discussed.


Isolation and properties of suppressor-sensitive mutants of Bacillus subtilis bacteriophage SP02. Suppressor-sensitive mutants of Bacillus subtilis phage SPO2 were isolated and classified into 18 cistrons. A linear genetic map was constructed. The genes controlling early functions of phage development were clustered near the CI gene which may produce phage repressor.


Examination of xylitol.

Examination of xylitol.


Mental illness and the tuberculosis patient.


A multiple origin for plastids and mitochondria. The impressive homologies between mitochondria and plastids, on the one hand, and procaryotic organisms, on the other, make it almost certain that these important cellular organelles had their origin as independent organisms. The vast number of symbiotic relationships of all degrees of evolutionary antiquity which have been found in contemporary organisms point to the ease with which such relationships can be established.


Trypanosomiasis.

Trypanosomiasis.


Effect of altered hormonal states on the histochemical distribution of succinic dehydrogenase activity in the rat prostatic complex.


A combined histochemical procedure to distinguish between mesenchymal and epithelial reticular cells in the rat thymus under the light microscope.


Recent work on scrapie.

Recent work on scrapie.


Typing of fetal cells obtained by amniocentesis.


Diagnostic experiences with the naphthol AS-D chloroacetate esterase reaction.


Anti-C as a naturally-occurring antibody.


Immunologic characterization of anti-H isohemagglutinins.


A materialistic look at periodontal packs.


Proof of existence of oximase activity in leaf of higher plants.


Purification and physical characteristics of a trypsin-like protease from the rat submandibular gland.


A new type of carotenoid pigment isolated from myxobacteria.


LCM virus as a carrier of non-viral cellular components. Electron microscopic evidence.


The causes of blindness in 156 visually and mentally defective children.


Norrie's disease.

Norrie's disease.


Clinical demonstrations.

Clinical demonstrations.


Application of the immunodiffusion procedure to rapid identification of arboviruses and to detection of specific antibody in sentinel animals.


Studies on fibrinolytic activity in normal persons and patients with atherosclerotic vascular disease after physical activity and injections of nicotinic acid.


The effect of stepwise doses of digitalis on hemodynamic function in man.


Subacute sclerosing panencephalitis. Encephalitis in hamsters produced by viral agents isolated from human brain cells.


Tubal and cervical cultures in acute salpingitis with special reference to Mycoplasma hominis and T-strain mycoplasmas.


Relative incidence of Corynebacterium vaginale (Haemophilus vaginalis), Neisseria gonorrhoeae, and Trichomonas spp. among women attending a venereal disease clinic.


Use of trimethoprim to prevent overgrowth by Proteus in the cultivation of N. gonorrhoeae.


Experience with a rapid direct immunofluorescent test for the gonococcus as a "bench" procedure in venereal disease clinics.


Demethylchlortetracycline compared with penicillin in the treatment of gonorrhoea in women.


Treatment of gonorrhoea with trimethoprim-sulphamethoxazole in Uganda.


Potentiation of responses to histamine in aortic strips by steroids.


In vitro response to oxygen of human umbilical arteries and of animal ductus arteriosus.


Kinetic properties of liver and prostate acid phosphatase in rabbit and monkey.


Nitrous oxide as a sensitizer of bacterial spores to x-rays.


Morphological study of microgametogenesis of Leucocytozoon simondi.


Intrauterine rubella infection following immunization with rubella vaccine.


Observation of immunoglobulins in the course of a tumour disease.


The organization and function of the bacterial chromosome. Regulation of chromosome replication in Bacillus subtilis. The effect of amino acid starvation in strain 168. ANL-7635.


An enzymatically inactive catalase-anticatalase. ANL-7635.


The organization and function of the bacterial chromosome. The chromosome in competent cells of Bacillus subtilis, strain 168. ANL-7635.


Immunochemistry and immunology of collagen and gelatin.


Left posterior fascicular block in canine and primate hearts. An electrocardiographic study.


Therapy of angina pectoris with propranolol and long-acting nitrates.


In-vitro comparison of erythromycin, lincomycin, and clindamycin. The in-vitro antibacterial activities of erythromycin, lincomycin, and clindamycin, a new derivative of lincomycin, were compared. Clindamycin was always more active than lincomycin, and was either as active as erythromycin or more so against betahaemolytic streptococci, Streptococcus viridans, Str. pneumoniae, and erythromycin-sensitive Staphylococcus aureus. It was also fully active against most erythromycin-resistant strains of Staph. aureus. On the other hand, it was somewhat less active than erythromycin against Haemophilus influenzae and considerably less active than erythromycin against Str. faecalis and Neisseria gonorrhoeae.Clinical trials seem to be justified in infections with sensitive organisms for which erythromycin might have been indicated.


Reovirus antibodies in Delhi.

Reovirus antibodies in Delhi.


Deficiency of C1r in human serum. Effects on the structure and function of macromolecular C1. THE EXPERIMENTS PRESENTED HERE UTILIZE A HUMAN SERUM MARKEDLY DEFICIENT IN HEMOLYTIC COMPLEMENT ACTIVITY TO SHOW THAT: (a) The hemolytic deficiency is the result of a selective deficiency in hemolytic C1. (b) The relative absence of hemolytic C1 is due to a profound deficit in C1r function associated with less than normal C1s protein and hemolytic function and normal C1q protein concentration and function. This deficit in C1r in the face of normal C1q suggests that different cell types are responsible for the synthesis of each of these components. (c) Whatever the basis for the deficiency of C1r function, this defect results in an inadequate association of the remaining C1 subcomponents, C1q and C1s, even in the presence of calcium ions, thus suggesting that C1r has an important role in the assembly and/or maintenance of macromolecular C1.


Differential expression of bacteriophage genomes in vegetative and sporulating cells of Bacillus subtilis. Two antigenically distinct bacteriophages, beta3 and beta22, have been isolated and characterized with Bacillus subtilis strain W23 as a host. They differ in plaque morphology, single-step growth characteristics, host range, and thermal stability. The deoxyribonucleic acids isolated from beta3 and beta22 differ in base composition, density in CsCl and Cs(2)SO(4), sedimentation coefficient, molecular weight, and thermal denaturation temperature. These phages have been used to analyze the ability of B. subtilis to sporulate despite infection by virulent phages. When development of phages beta3 and beta22 in sporulating cultures was compared with that in log cultures, an increase in the latent periods of infection and a decrease in the burst sizes for the two phages were observed. Sporulating cultures infected with beta3 yielded the usual percentage (85%) of mature spores; 80% of these contained phage determinants and 20% were uninfected. However, cultures infected with beta22 lysed. Of the small fraction (0.01%) which sporulated, 83% were uninfected and 17% were infected. Phage beta3-infected and uninfected spores were examined to distinguish any chemical or physical differences. Preparations of both types of spore contained 81.4 mug of dipicolinic acid per mg (dry weight), and examination by phase-contrast microscopy gave no evidence of any difference in outward appearance. A 20% decrease in infected spore count was observed upon heating at 80 C for 10 min. Differences in the infection processes of the two phages prompted an analysis of the transcription process after infection. Deoxyribonucleic acid-ribonucleic acid hybrid analysis of relative amounts of phage-specific and host-specific messenger ribonucleic acid (mRNA) present in infected cells suggested that beta3 was unable to repress the synthesis of host mRNA and that beta3-specific mRNA synthesis was repressed in sporulation-phase cultures. Phage beta22, in contrast, was able to repress host-specific mRNA synthesis in both log-infected and sporulation-infected cells. The results suggest that the differential expression of the phage genomes is due to the relative ability of the phages to repress the host genome.


Care of the high-risk infant in the intensive care unit.


Comparison of methods of peripheral nerve suturing in monkeys.


Silicone fluid research. A follow-up summary.


Heredity and strabismus.

Heredity and strabismus.


Partial loss of antigenicity of 6C 3HED ascites tumor cells after preservation over liquid nitrogen.


The distribution of thirteen H-2 alloantigenic specificities among the products of eighteen H-2 alleles.


Aversive quality of low velocity winds at various ambient temperatures.


Resistance to bacterial infection in a hypobaric normoxic environment.


Malnutrition studies in the rhesus monkey. I. Effect on physical growth.


Structure of phenylalanine-specific transfer ribonucleic acid from baker's yeast.


Recent chemistry and biochemistry of bile pigments.


Synthesis and conformation of collagen models.


Synthesis and reactions of penicillin-like compounds.


Therapeutic effectiveness of homologous erythrocyte transfusions following frozen storage at --80 C for up to seven years.


Distribution of integumental tyrosinase activity in red-eared turtle, Pseudemys scripta elegans.


Renal regeneration in chloroform-poisoned male mice of strain C3H-He treated with di-sodium versenate.


Autoradiographic localization of 5-hydroxytryptamine in monkey pineal gland.


Atypical muscle cells in the wall of the renal calix and pelvis with a note on their possible significance.


Immunosuppressive effect of chronic strontium-90 administration to miniature swine.


Radio-sensitization with iodine compounds. I. Examination of damage in deoxyribonucleic acid with Bacillus subtilis transformation system by irradiation in the presence of potassium iodide.


A radiobiological study of interferon formation effects of monochromatic U.V. rays and effects of U.V. rays on bromodeoxyuridine-substituted cells.


A new mucopolysaccharidosis.

A new mucopolysaccharidosis.


Excretion of Na+,K+,Cl-, and HCO3- in the saliva of the rhesus monkey.


Dissociation of noncovalently held polymers of human and rabbit IgM.


Comparison of septic shock due to gram-negative and gram-positive organisms.


Sensitive test for detection of early viral myocarditis in mice.


Effect of injury on antigenic composition of rabbit skin.


Repetitive ventricular response (RVR) in the digitalized heart of various mammalian species.


Quantitative biological assay of bacterial endotoxins.


Effects of poly I-C on the course of infection with Trypanosoma cruzi.


The extension of the concept of tissue-air ratios (TAR) to high-energy x-ray beams.


Circadian rhythmic amplitude modulation of Berger-region frequencies in electroencephalograms from "Macaca mulatta".


Heart rate, blood pressure, and electrocardiographic changes during provoked attacks of cluster headache.


Insulin resistance in juvenile diabetes mellitus. Immunologic studies.


Hereditary nephritis with nerve deafness. Immunofluorescent studies on the kidney, with a consideration of discordant immunoglobulin-complement immunofluorescent reactions.


Dental development in the cotton ear marmoset (Callithrix jacchus).


Main patterns and individual differences in baboon skull split-lines and theories of causes of split-line orientation in bone.


Hemoglobins of baboons, Papio cynocephalus, P. gelada and P. hamadryas.


The social organization of Barbary apes (Macaca sylvana) on Gibraltar.


Further studies of sympathetic actions of anaesthetics in intact and spinal animals.


Chronic paronychia.

Chronic paronychia.


Visceral complications of supervoltage radiotherapy for carcinoma of the bladder.


Unusual cold abscesses.

Unusual cold abscesses.


Disaggregation of slime mold pseudoplasmodia using EDTA and various proteolytic enzymes.


The micronucleus of the ciliate Stylonychia mytilus; its nucleic acid synthesis and its function.


RNA metabolism in the macronucleus of Euplotes eurystomus during the cell cycle.


Macromolecular synthesis during regeneration in Blepharisma determined by specific inhibitors and incorporation of 14C-tracers.


Ethylene diamine tetra-acetic acid and the bactericidal efficiency of some phenolic disinfectants against Pseudomonas aeruginosa.


Factors affecting the response to insulin in the normal subhuman pregnant primate. The concentrations of plasma glucose, free fatty acids, insulin, growth hormone, and placental prolactin in subhuman primate fetal and maternal plasma were examined following intravascular administration of insulin and glucagon to the fetus and mother. The neonatal plasma responses to these same stimuli were also examined. Fetal plasma glucose concentrations were minimally altered by direct fetal insulin injections, whereas neonatal glucose levels declined with similar injections. In both instances, however, plasma free fatty acid levels declined following insulin. When the amount of insulin given the fetus was increased, fetal plasma glucose concentrations did decline. Combined intravascular insulin injections and infusions in the mother were associated with a disappearance of the initial maternal to fetal plasma glucose concentration gradient and a nearly parallel fall in both maternal and fetal plasma glucose levels. It was concluded that insulin was biologically active in the fetus. Obtunded fetal plasma glucose responses to direct fetal insulin administration may be a function of placental transfer of glucose from the maternal pool. Maternal plasma placental prolactin and fetal plasma growth hormone levels were unchanged in the presence of sustained maternal and fetal hypoglycemia. However, neonatal plasma growht hormone levels did increase in response to hypoglycemia. The observed bidirectional placental barrier to transfer of radioisotopically labeled growth hormone indicated that fetal plasma growth hormone was solely of fetal origin. These data suggested further that a change in the growth hormone-releasing mechanism may occur from fetal to neonatal life. Direct maternal intravascular glucagon administration led to augmentation in both maternal and fetal plasma insulin and glucose levels. Direct fetal glucagon injections enhanced both maternal and fetal plasma insulin levels. These simultaneous changes in both plasma pools were consistent with the demonstration of a bidirectional placental transfer of radioisotopically labeled glucagon. The role of endogenously produced glucagon in these studies remains to be clarified.


The effect of mechanical agitation and CO2 on the growth of the BCG, H37Ra and RlRv strains of Mycobacterium tuberculosis.


Shrinkage of the bacterial cell following treatment with various fixatives.


Response of trigeminal ganglion neurons to thermal stimulation of oral-facial regions. I. Steady-state response.


Response of trigeminal ganglion neurons to thermal stimulation of oral-facial regions. II. Temperature change response.


Discharge of cerebellar neurons related to two maintained postures and two prompt movements. I. Nuclear cell output.


Discharge of cerebellar neurons related to two maintained postures and two prompt movements. II. Purkinje cell output and input.


Mental integrity and the nature of life.


The doctor and hospital costs.

The doctor and hospital costs.


Metabolism of lipids in the atherosclerotic Rhesus monkey aorta.


Induction of complicated arteriosclerotic lesions in experimental animals.


Changes in thyroxine-binding globulin levels in thyrotoxicosis and in healthy subjects after triiodothyronine administration.


Studies on the inactivation of pancreozymin concentrates.


The virulence of tubercle bacilli for guinea pigs and the susceptibility of guinea pigs to tubercle bacilli.


Isolation and properties of bacteriocin-tolerant mutants of Klebsiella edwardsii var.


Silicone-rubber electrodes for long-term patient monitoring.


Development of a chemically defined medium for growth of Neisseria gonorrhoeae.


Angina without coronary disease (sic).

Angina without coronary disease (sic).


Some mechanisms of action of stanozolol (stromba) and its interaction with cortisone.


Thiothixene in the treatment of anxiety and depression in outpatients.


Chromosomal location and properties of radiation sensitivity mutations in Bacillus subtilis. Transformation and transduction crosses involving recA1, recB2, and urv-1 mutations have shown that these mutations belong to three distinct unlinked genetic loci. The precise position of these loci on the Bacillus subtilis chromosome map has been determined. The behavior of recB2 strains in transformation studies suggested a dominance of recB2(+) function over recB2 and an early expression of this phenotype during transformation. Strains bearing two ultraviolet sensitivity markers possess a phenotype characteristic of the marker with the most adverse effect on recombination. The possibility that the effects of the two mutations are additive was also considered. Results are also presented which show that a phage-induced enzyme is not responsible for the high transducibility of recA1 strains.


Cell wall polymers of Bacillus sphaericus 9602. II. Synthesis of the first enzyme unique to cortex synthesis during sporulation. The cell wall peptidoglycan of vegetative cells of Bacillus sphaericus 9602 contains l-lysine and d-isoasparagine and is devoid of diaminopimelic acid (Dap), whereas the peptidoglycan of its spore cortex is devoid of l-lysine and d-isoasparagine and contains meso-Dap. These two structures have a common biosynthetic precursor, uridine-diphospho-N-acetylmuramyl-l- alanyl-d-glutamic acid, which accepts either l-lysine or meso-Dap, the latter reaction being the first unique to the synthesis of the spore cortex peptidoglycan. l-lysine-adding activity decays at the end of vegetative growth to a level which is maintained until Dap-adding activity appears, when it declines rapidly again. Dap-adding activity is not detectable in refractile spores, in vegetative cells, or in sporulating cells until about 4 hr after the end of vegetative growth, when it increases rapidly for about 1.5 hr in a process dependent on continued protein and ribonucleic acid (RNA) synthesis. This process apparently involves transcription and translation during this period of a "sporulation-specific" gene whose product is essential for and unique to sporulation. It is closely followed by the acquirement of refractility. Another sporulation-specific gene, that for dipicolinate synthase, is apparently transcribed and translated in an overlapping period commencing about 0.5 hr later, although dipicolinate does not accumulate rapidly until 1.5 hr later, when about 75% of the cells are already refractile. Inhibition of protein synthesis with chloramphenicol or of RNA synthesis with streptolydigin inhibited accumulation of these enzymes in sporulating cells; this inhibition could be reversed by washing out the antibiotics after 1.5 hr. Sporulation recommenced with an unaltered sequence of events but with poorer synchrony. There was no evidence for a messenger RNA for either enzyme of lifetime greater than a small fraction of the period of enzyme accumulation, although dilution with 10 volumes of fresh medium failed to prevent synthesis of Dap-adding enzyme in cells which had become terminally swollen, a process preceding enzyme synthesis by about 1.5 hr. The synthesis of this enzyme in B. sphaericus is apparently dependent on programmed transcription of the appropriate gene.


Metabolism of phospholipid 2-linked fatty acids during the release of membrane fragments from Haemophilus parainfluenzae by ethylenediaminetetraacetic acid-tris(hydroxymethyl)-aminomethane. Membrane fragments containing diacyl phospholipids were released from viable cells of Haemophilus parainfluenzae during incubation in ethylenediaminetetraacetic acid (EDTA)- tris(hydroxymethyl)aminomethane (Tris) buffer. The phospholipids located in the part of the membrane that was released during the EDTA-Tris treatment had markedly different proportions of fatty acids than the lipids remaining in the cell residue. Very little metabolism of the 1-linked fatty acid occurred. After a short pulse with (14)C, the specific activity of the 1-linked fatty acid was lower in the phospholipids released than in the phospholipids of the residue, indicating an earlier time of synthesis of those lipids released in the membrane fragments. During the EDTA-Tris treatment, the 2-linked fatty acid was metabolized. This metabolism may have involved phospholipase A(2) which stimulates the synthesis of fatty acids and the transfer of acyl groups to the lysophospholipid.


Carbonic acid from decarboxylation by "malic" enzyme in lactic acid bacteria. Carbonic anhydrase studies were used to determine the primary form of carbonic acid produced from decarboxylation of l-malic acid by "malic" enzyme in malolactic strains of five different species of lactic acid bacteria. Addition of carbonic anhydrase to the reaction mixture containing crude bacterial extract and l-malic acid, at pH 7, in all five cases resulted in an increase (13 to 23%) in the rate of carbon dioxide evolution over the control. The results indicated that the primary form of carbonic acid released from "malic" enzyme was not anhydrous carbon dioxide as previously supposed and as has been shown for other decarboxylating enzymes. The standard free-energy changes of the malo-lactic reaction with the various forms of carbonic acid as the primary decarboxylation product were calculated. The reaction is less exergonic when carbonic acid, bicarbonate ion, or carbonate ion is the primary decarboxylation product compared to anhydrous carbon dioxide. The free-energy of the reaction is not biologically available to the bacteria; with carbon dioxide not the primary decarboxylation product, the potential energy lost in a malo-lactic fermentation is not as great as previously considered. Endogenous carbonic anhydrase activity was not found.


Interaction of concanavalin A with an arabinogalactan from the cell wall of Mycobacterium bovis. The arabinogalactan isolated from the cell wall of Mycobacterium bovis reacts to form a precipitate with concanavalin A, the phytohemagglutinin of the jack bean. A typical precipitin-like curve is obtained when increasing amounts of the polysaccharide are added to a constant quantity of concanavalin A. Inhibitor studies suggest that concanavalin A reacts with the arabinogalactan by interacting with the C-2, C-3, and C-5 hydroxyl groups of the alpha-d-arabinofuranosyl residues situated at the chain ends of this polysaccharide.


Relationship between the location of autolysin, cell wall synthesis, and the development of resistance to cellular autolysis in Streptococcus faecalis after inhibition of protein synthesis. Ten minutes after inhibition of protein synthesis with chloramphenicol (CAP) the ability of cells of Streptococcus faecalis (ATCC 9790) to autolyze decreased to less than 20% of the rate for exponential-phase cells. After threonine exhaustion, the time for a 50% drop in the rate of cellular autolysis was about 20 min. These rapid increases in resistance to cellular autolysis could not be accounted for by: (i) the relatively slow and small overall decrease in susceptibility of isolated cell walls to added autolysin, or (ii) a decreased content of either the active or latent (proteinase activatable) form of the autolysin in the wall fraction. Continued wall synthesis resulted in dilution of preexisting autolysin in the isolated wall fraction. The release of labeled "old" relative to "new" wall from CAP-treated cultures showed that wall synthesis shifted away from the areas of wall previously shown to be associated with wall synthesis (extension) in exponential-phase cells. A corresponding dispersal of active autolysin activity was not observed. By using actinomycin D and CAP, a requirement for ribonucleic acid and protein synthesis early in the recovery of cells from amino acid starvation was demonstrated for the recovery in the ability of cells to autolyze. Evidence was obtained which suggests that a protein is involved in the conversion of latent to active autolysin. During recovery from amino acid starvation, increase in wall synthesis and content of active autolysin was delayed (25 to 35 min), whereas an increase in turbidity and latent enzyme content began within 10 min. After treatment with CAP at 22 or 52 min of recovery, a further increase in levels of both active and latent autolysin was severely inhibited; however, the increase in rate of wall synthesis was indistinguishable from that of an untreated control. This suggests that an increase in rate of wall synthesis does not depend on an increase in level of active autolysin.


Factors affecting deoxycholate inactivation and Mg++ reactivation of Bacillus megaterium KM membrane nicotinamide adenine dinucleotide (reduced form) oxidase. Decay of the reduced nicotinamide adenine dinucleotide oxidase of Bacillus megaterium KM membranes was prevented by storage in 10% (v/v) glycerol or 0.4% bovine serum albumin. Differential rates of solubilization of components of the oxidase system by 0.4% deoxycholate was demonstrable at 4 C. The amount of Mg(++) necessary for maximal oxidase reactivation increased with increasing amounts of deoxycholate-inactivated oxidase. Mg(++) activation of deoxycholate-inactivated oxidase was partially temperature-dependent. Maximal activation was observed at 37 C, but only partial activation took place at 4 C. A small amount of deoxycholate was required for Mg(++) activation of deoxycholate-inactivated oxidase. Two pH optima were found for Mg(++) activation of deoxycholate-inactivated oxidase, pH 5.3 and 7.3. Significant amounts of activation of the inactive oxidase occurred in the absence of Mg(++) with an optimum at pH 5.0, with essentially no Mg(++)-independent activation demonstrable at pH 7.0.


Cell wall binding properties of the Bacillus subtilis autolysin(s). Cell walls isolated from exponentially growing Bacillus subtilis have autolysin(s) attached to them. An autolysin can be released from the walls by incubation at 0 C with 3 m LiCl. The enzyme can reattach to walls when the salt concentration is reduced. The bound enzyme cannot be removed or destroyed by washing the walls with 8 m urea at 0 C. The binding of free enzyme to walls at 0 C can take place normally in the presence of 2 m urea.


Autolysin(s) of Bacillus subtilis as dechaining enzyme. Bacillus subtilis bacteria grow exponentially as long filaments in well-aerated cultures at 48 C. These filaments are chains of cells which can become unlinked when a B. subtilis autolysin or egg white lysozyme is added to the culture medium.


Site of initiation of cellular autolysis in Streptococcus faecalis as seen by electron microscopy. Low concentrations of glutaraldehyde (0.1% or higher) blocked cellular and wall autolysis. The site of autolytic activity was studied by allowing cell autolysis to proceed for very short periods (0 to 15 min) before addition of glutaraldehyde. Electron microscopy of ultrathin sections showed that the primary site of autolytic activity was the leading edge of the nascent cross wall. The base of the cross wall seemed more resistant than the tip. Evidence supporting the involvement of autolysin activity in continued wall extension and in cell separation as well as in the initiation of new sites of wall extension was obtained. In cells exposed for 10 min to chloramphenicol, wall dissolution was very much slower but occurred at the same cross wall site.


Relationship of dipicolinic acid content in spores of Bacillus cereus T to ultraviolet and gamma radiation resistance. Spores of Bacillus cereus T lacking dipicolinic acid showed a statistically significant reduction in resistance to ultraviolet and gamma radiation as compared with spores with high dipicolinic acid content.


Complexing of fatty acids by Triton WR1339 in relation to growth of Mycobacterium tuberculosis. Mycobacterium tuberculosis grew in the presence of toxic concentrations of free fatty acids when an adequate amount of triton WR1339 was added to the medium. Triton produced a solubilizing effect on turbid suspensions of fatty acids, indicating the formation of a detergent-lipid complex. Oleic acid complexed with triton served as a source of carbon for growth of M. tuberculosis.


Quantitation of the action of ethylenediaminetetracetic acid and tris(hydroxymethyl)aminomethane on a gram-negative bacterium by vancomycin adsorption. Changes in the cell wall of a species of Flavobacterium can be quantitated by measuring vancomycin adsorption. Treatment of the cells with ethylenediaminetetraacetic acid or tris(hydroxymethyl)aminomethane (or both) increased adsorption of the antibiotic.


Evidence for immunological identity of human and simian (baboon) TSH in serum.


Neonatal suppurative parotitis.

Neonatal suppurative parotitis.


The appearance and destruction of platelet-clumping substance in serum added with trypsin.


Effect of larval cuticle on the activation of phenoloxidase in Drosophila melanogaster.


Interference and inferotemporal memory deficits.


Nystagmus induced by stationary repetitive light flashes in monkeys.


Bacteriophage infection: which end of the SP82G genome goes in first? The transfer of deoxyribonucleic acid (DNA) from bacteriophage SP82G to its host may be halted by chilling but is affected little by chloramphenicol, actinomycin D, or cyanide. The order of entry of markers on the phage genome was determined by halting the transfer of DNA at intervals, removing the untransferred DNA by blending, and assaying for the presence of markers in the blended complexes. Markers on the phage genome are transferred in a linear, polar fashion consistent with the previously determined genetic and physical maps. Those markers concerned with early functions enter first, and the rate of transfer is temperature dependent.


Disruption of adenovirus type 7 by lithium iodide resulting in the release of viral deoxyribonucleic acid. Adenovirus type 7 exposed to solutions of LiI was progressively converted into slower sedimenting deoxyribonucleic acid (DNA)-containing particles, and, ultimately, under proper conditions, DNA free or almost free from protein was released from the virus. The degree of viral degradation was dependent on the time of treatment, on the temperature, and on the concentration of the reagent.


Characterization of inducible bacteriophages in Bacillus licheniformis. Two morphologically distinct and physically separable defective phages have been found in Bacillus licheniformis NRS 243 after induction by mitomycin C. One of them (PBLB) is similar to the defective phage PBSX of B. subtilis, which has a density of 1.373 g/cm(3) in CsCl and a sedimentation coefficient of 160S. PBLB incorporates into its head mainly bacterial deoxyribonucleic acid (DNA) which has a sedimentation coefficient of 22S and a buoyant density in CsCl of 1.706 g/cm(3). The other phage (PBLA) has a morphology similar to the temperate phage phi105 of B. subtilis; the head diameter is about 66 nm, and it possesses a long and noncontractile tail. PBLA has a density of 1.484 g/cm(3) in CsCl and the phage-specific DNA, which is exclusively synthesized after induction by mitomycin C, has a density of 1.701 g/cm(3). PBLA DNA is double-stranded and has a sedimentation coefficient of 36S, corresponding to a molecular weight of 34 x 10(6) to 35 x 10(6) daltons. The phage DNA has one interruption per single strand, giving single-stranded segments with molecular weights of 13 x 10(6) and 4 x 10(6) daltons. Common sequences between the two phage DNA species and with their host DNA have been demonstrated by DNA-DNA hybridization studies. Both phage particles kill sensitive bacteria. However, all attempts thus far to find an indicator strain to support plaque formation have been unsuccessful.


Effect of iron on the bactericidal proteins from rabbit polymorphonuclear leukocytes.


Selected examples of structure determination of natural carotenoids.


Evidence for the double-stranded nature of the RNA of Colorado tick fever virus, an ungrouped arbovirus.


Electrical stimulation and steady potential shifts in prefrontal cortex during delayed response performance by monkeys.


Studies on a monkey placental protein with immunochemical similarity to human growth hormone and human chorionic somatomammotrophin.


ABO and Rh incompatibility. I. Fetal and neonatal mortality aith ABO and Rh incompatibility. Some new interpretations.


ABO and Rh incompatibility. II. Is there a dual interaction in combined ABO and Rh incompatibility?


Potentiometric determinations of calcium, magnesium, and complexing agents in water and biological fluids.


Controversy in human genetics. Blood groups and disease.


The apophyses of the lateral malleolus.

The apophyses of the lateral malleolus.


Adjuvant arthritis in the rat. Effect of intraperitoneal injections of either whole dead mycobacteria or tuberculin.


Gastrotomy with double gastrostomy in the performation of the esophagus.


Studies of the nasal vasculature, and the problems of arterial ligation for epistaxis.


Parenteral nutrition and esophageal anastomotic leak.


Agglutination in agar gel of group A and B particulate antigens obtained by sonic vibrations.


Specific dental responsibilities in the cleft palate team and coordinating of dental care: long-term planning.


Adenyl cyclase of rat parotid gland. Activation by fluoride and norepinephrine.


Antibacterial immunity induced by ribosomal vaccines.


The effects of suntanning.

The effects of suntanning.


Carcinogenesis in tissue culture. XI. Cytotoxic effects of 4-dimethylaminoazobenzene and its derivatives on HeLa cells and on rat liver and kidney cells in culture.


Studies on the micro-quantitation of antibody by the reversed type single radial immunodiffusion.


Immunochemical properties of lipid protein complex. II. Antigenicity of human serum low density lipoprotein and its derivatives.


Studies on the chemical structure of serologically active arabinomannan from mycobacteria.


Form and characteristics of the cardiovascular conditional response in Rhesus monkeys.


Waldenström's macroglobulinemia. Localization in ileum and lacrimal glands.


An ultramicromethod for serum calcium determination.


Elimination of error in hematocrit produced by excessive EDTA. Experience with the Coulter Counter Model S.


Social nutrition and malnutrition.

Social nutrition and malnutrition.


Antibiotic synergism of enterococci. Relation to inhibitory concentrations.


Diagnosis of tuberculous pleurisy by culture of pleural biopsy specimen.


Bilateral depression in photic-evoked response as a late effect of unilateral visual cortex x-irradiation.


Effects of dorsolateral frontal cortex lesions and discrimination-alternation training on regional acetylcholinesterase activity in monkey.


Comparison of four culture media for the isolation of Neisseria gonorrhoeae: correlation with the direct smear method.


The chemical and immunological assay of low density lipoproteins extracted from human aortic intima.


Lipid changes in the plasma lipoproteins of baboons given an atherogenic diet. I. Changes in the lipids of total plasma and of alpha and beta-lipoproteins.


Treatment of status epilepticus.

Treatment of status epilepticus.


The number of Purkinje cells after diphenylhydantoin intoxication in monkeys.


The effects of immobilization on spermatogenesis in subhuman primates.


The effect of bovine growth hormone on zinc-65 metabolism and prostatic blood flow of intact, testosterone treated, and castrated adult male Chacma baboons.


Cytochemical localization of peroxidase acitivity in the submandibular salivary gland of the hamster.


Urinary excretion of radio-labeled oxalate precursors by normal and oxalate stone-forming persons and by a primary hyperoxaluric before and after calcium carbimide therapy.


Antibacterial correlates of urine drug levels of hexamethylenetetramine and formaldehyde.


The salivatory nuclei in the brainstem of the monkey (Macaca mulatta).


Storage of meningococcal and streptococcal L-forms.


Age changes of the sodium rhodanide compartment in piglets.


Biochemical analysis of normal camel blood.


Cancer of the urinary tract.

Cancer of the urinary tract.


Surgical feeding procedures in patients with neurological disorders.


Sinus bradycardia treated by long-term atrial pacing. A technique for long-term atrial pacing is described. It uses the system whereby power from an external pulse-generator (including battery) is transferred through the intact skin by inductive-coupling. Once installed, there is no requirement for further surgery to effect battery changes, and the patient can control his heart rate over a wide range to suit his needs. It is suggested that pacing by such means may offer a preferable alternative to drug therapy in the treatment of low cardiac output due to pathological sinus bradycardia. Two patients successfully treated in this way are reported.


Autoimmunity and the retina. I. Antigenic specificity of photoreceptor cells.


Vasogenic origin of visual field defects and optic nerve changes in glaucoma.


Dose conversion factors for use with ionisation dosemeters for 6 to 35 MeV electron beams.


A general expression for megavoltage central axis depth doses.


Ossified bronchial adenoma.

Ossified bronchial adenoma.


The international standard for anti-smallpox serum. At the request of the WHO Expert Committee on Biological Standardization, the National Institute for Medical Research, London, obtained 63 samples of convalescent human plasma from patients recovering from smallpox. The sera were pooled and distributed into ampoules and freeze-dried.The freeze-dried material was examined in 11 laboratories in 6 countries in an international collaborative assay during 1964 and 1965.The results from 10 laboratories were more or less consistent, but relative potencies in terms of the proposed international standard estimated by one of the laboratories were exceptionally low. Since this laboratory used a strain of virus different from that used in all the other laboratories, it was suspected that antigenic properties or avidity of the virus might have caused the divergence of results; this is being investigated.After considering the report of the international collaborative assay, the WHO Expert Committee on Biological Standardization established the material as the International Standard for Anti-Smallpox Serum, and a unitage was assigned so that 1 International Unit of activity was contained in 0.08416 mg, and each ampoule contains (on average) 1000 IU of activity.


Carbohydrate metabolism in pseudomonas infection.


Experimental ovine listeriosis. IV. Pathogenesis of fetal infection.


Observations on a new astomate ciliate, Anoplophrya pheretimii from the earthworm, Pheretima posthuma (L. Vaill.). II. Cytochemistry.


Cerebral representation of vocalization in the squirrel monkey.


Immunologic consequences of cryosurgery. I. Serum proteins.


A survey of recent research on the mode of action of intra-uterine contraceptive devices.


Control of contractility in Spirostomum by dissociated calcium ions. The freshwater protozoan, Spirostomum ambiguum, exhibits generalized contraction when electrically stimulated with a DC pulse. Light and electron microscopic studies show a subcortical filamentous network, believed responsible for generating contractile tension, in association with vesicles which were shown to accumulate calcium oxalate precipitates. Organisms microinjected with the calcium-sensitive, bioluminescent protein, aequorin, emit light when stimulated to contract. Analyses of cinefilm records of electrically induced contraction indicate that contraction may occur up to 25 msec after the onset of stimulation at a point when the calcium-aequorin light emission is at a peak. The evidence shows that calcium release from an interval compartment is directly associated with the onset of contraction in Spirostomum, and that the removal of calcium, through some internal sequestering mechanism, signals relaxation.


Pathogenesis and reversibility of the pulmonary lesions of oxygen toxicity in monkeys. II. Ultrastructural and morphometric studies.


Pathogenesis and reversibility of the pulmonary lesions of oxygen toxicity in monkeys. I. Clinical and light microscopic studies.


Pathology of experimental enterotoxemia. The in vivo localization of staphylococcal enterotoxin B.


Suicidal population of a military psychiatric center. A review of ten years.


Suicides and attempted suicides in the Norwegian Armed Forces during peace time.


The phenoloxidases of the ascomycete Podospora anserina. Communication 4. Genetic regulation of the formation of laccase.


The problem of vivax malaria in Vietnam returnees. II. Malaria chemoprophylaxis survey.


Sulfadiazine and sulfadiazine-penicillin in mass prophylaxis of meningococcal carriers.


A method of relating physiology and military performance. A study of some effects of vapor barrier clothing in a hot climate.


Long-lasting lozenges: dissolution studies with CU 701-1 in patients with oral lesions and in volunteer (normal) subjects.


The risk of exposure to a patient with meningococcal meningitis.


United States Army Aviation Mediciine in the Republic of Vietnam.


A case of aural myiasis caused by Cochliomyia macellaria (Fabricius).


Quality assurance and pharmacological equivalency.


A field-expedient C-ration sleeve litter.


The provision of medical care.

The provision of medical care.


The Massa hoax.

The Massa hoax.


Observations on the developmental relations between ectoderm and mesoderm of the chick embryo tail.


Pathways to the cerebral cortex for nerve impulses from joints.


Formylation of methionyl-transfer RNA from prokaryotes and eukaryotes by Bacillus subtilis transformylase.


Transport and phosphorylation of glucose, fructose, and mannitol by Pseudomonas aeruginosa.


Comparison of molecular structures of proteins: helix content; distribution of apolar residues.


Selective tyrosine modification with cyanuric fluoride.


Epithelial glycoproteins of human gallbladder. Immunological characterization.


Multiple tumors in a Macaca mulatta monkey.


Dynamic state of calcium reserves in freshwater clam mantle.


Toxic effects of the sodium and the N,N'-dibenzylethylenediamine salts of tenuazonic acid (NSC-525816 and NSC-82260).


Carbonic anhydrase and mobilisation of calcium reserves in the mantle of lamellibranchs.


Comparison of a combination of 5-fluorouracil (NSC-19893), mitomycin C (NSC-26980), triethylenethiophosphoramide (NSC-6396), and fluoxymesterone (NSC-12165) with 5-fluorouracil alone in patients with advanced cancer.


Biologic activity of daunomycin (NSC-82151) in microbial systems.


Hyperbaric oxygen in radiotherapy of head and neck carcinoma.


Brain extracellular space in monkey fetuses subjected to prolonged partial asphyxia.


Immunochemical analysis of a uronic acid polymer of Staphylococcus epidermidis, strain 53.


Antigenic analysis of human heart tissue. Antigens with restricted organ distribution in acid extracts of human myocardium.


Dissociation of a small proportion of a human IgM population into Fcmu, Fcmu, and free light chains following reduction.


Variation in the size of antibody sites for the poly-L-aspartate hapten during the immune response.


Studies on brain antigens. IV. Isolation and partial characterization of a rat species-restricted antigen of nervous tissue (SRANT).


Cellular reactivity to tuberculin in immune and serologically-unresponsive guinea pigs.


Electroencephalography and other diagnostic procedures in epilepsy.


Anticonvulsant drugs in the management of epilepsy.


Solubilization of DNA in Bacillus subtilis during recovery from UV-irradiation.


A technique for assessing strength of a smoking preference in monkeys.


Progress in eradication of bovine brucellosis in Tasmania.


Antigenic relationship between Escherichia coli and Neisseria meningitidis.


Tuberculosis in the leopard frog (Rana pipiens).


Neurofibromatosis and hyperparathyroidism--a new syndrome?


Assessment of a lower extremity training program. The response to a lower extremity program has been studied in workmen undergoing progressive rehabilitation following injury to the lower leg or ankle region. When first seen, the isometric strength of the injured limb was substantially reduced relative to its counterpart, and aerobic power was also poor, relative to normal standards. Substantial gains of isometric strength occurred over a four- to six-week period of therapy. Measurements above the site of injury suggested a 13% gain of strength, and measurements below the injury indicated a gain of 34%. On the other hand, there were minimal changes of thigh circumference over this period. Soft tissue radiographs taken in the posteroanterior and lateral planes showed a small (1%) increase of muscle tissue and a somewhat larger (5%) loss of subcutaneous fat. Dynamic exercise at a fixed percentage of aerobic power was associated with similar subjective discomfort and a similar accumulation of lactate before and after rehabilitation.It is concluded that the apparent improvement in isometric strength over the period of rehabilitation is due to (1) initial voluntary limitation of effort and (2) increase in skill. The use of soft tissue radiographs may afford a more objective basis for the evaluation of future muscle training programs.


Fine structure of nerve endings in the carotid body.


Lysosome morphogenesis and function in cells of the nervous system.


The entry of vessels into the brain surface with a view to the fine surface configuration of the channel of entry.


Fine relief configuration of the brain surface as determined by the surface of the membrana limitans gliae superficialis.


Immediate-type hypersensitivity in dogs: cutaneous, anaphylactic, and respiratory responses to ascaris.


Effect of phenothiazines on melanoma tyrosinase activity.


Thalidomide: disposition in rhesus monkey and studies of its hydrolysis in tissues of this and other species.


Cardiovascular pharmacology of perhexiline.


Hypotensive effects of chlordiazepoxide, amobarbital and chlorpromazine on behaviorally induced elevated arterial blood pressure in the squirrel monkey.


What price national medical insurance?

What price national medical insurance?


Serum adenosine deaminase activity in typhoid and paratyphoid B fever.


Primary drug resistance (The second report).


Course of cases of pulmonary tubercurosis with bacilli resistant to streptmycin, para-aminosalicylic acid and isoniazid and with multiple drug-resistance.


Enzymologic evaluation of experimental marmoset hepatitis.


Insulin in serum.

Insulin in serum.


Excretion and metabolism of cinanserin-14C and its oxygen analogue, SQ 10,624-14C.


Sequential cleavage of dinucleotides from DNA by SP3 DNase. 3. Substrate specificity and initiation of the hydrolysis from the 5-terminus of polynucleotides.


Membrane synthesis in Bacillus subtilis. I. Isolation and properties of strains bearing mutations in glycerol metabolism.


Membrane synthesis in Bacillus subtilis. II. Integration of membrane proteins in the absence of lipid synthesis.


Studies on membrane synthesis in Bacillus megaterium KM.


Immunoglobulin (IgG, IgA and IgM) levels in primary carcinoma of the liver and cirrhosis of the liver in Singapore.


Anticoagulants after open-heart surgery.


Disturbance of nourishment of the articular cartilage and its relation to the development of osteoarthrosis.


Immunological studies on human antithrombin 3. Influence of age, sex and use of oral contraceptives on serum concentration.


Cardiomyopathies of obscure origin in Cali, Colombia. Clinical, etiologic, and laboratory aspects.


Velocity of coronary sinus blood flow as an indicator of coronary arterial flow.


CW (RHW) inheritance in an Amish isolate.


Hemodynamic and respiratory effects of dopamine on septic shock in the monkey.


PCO2 and PNH3 in mammalian kidney and urinary tract related to urine pH and flow.


A study of the attachment phase of phagocytosis by murine macrophages.


Increased capillary permeability to 131-iodide and [51Cr]EDTA in the exercising forearm of long-term diabetics.


The effect of isomeric cis-octadecenoic acids on the growth of monkey kidney cells (LLC-MK2).


The effect of oleic acid on the growth of monkey kidney cells (LLC-MK2).


Jelly coat substances of sea urchin eggs. I. Sperm isoagglutination and sialopolysaccharide in the jelly.


Functional investigations of liver by means of labelled with 131J Rose Bengal in acute metabolic alkalosis in rabbits.


Radioindicator investigations with regard to the effect of atropine, buscolysin and papaverine on liver function.


Antifungal activity of natural Mericleri and Karlsbad salt in vitro.


Comparative histological investigations on the evacuated uterine mucous membrane in the vacuum-excochleation and classical methods of interruption of pregnancy. The author has investigated comparatively the degree of injury on the walls of the uterine cavity using the vacuum-excochleation method and classical methods of pregnancy interruption.  The evacuated contents resulting from abortions in 823 patients were studied histologically.  It was found that with the vacuum-excochleation method, the mucous membrane was separated in the spongiose in 66.2% of the cases, injury of basal layer occurred in 8%, muscular layer injury  was found in 4.2%, and a deep injury of the  muscular layer was found in .4%.  With the classical method of pregnancy interruption, the incidence of injury of the basal layer was 24.3%, myometrial injury was found in 35.4%, and a deep injury of the muscular layer occured in 15.2% of the cases.  The depth of injury of the mucous membrane with the vacuum-excochleation method depends on: 1) the strength of vacuum, 2) the model of the instrument; and 3) the size of the terminal aperture of the vacuum-curet.


Changes in the lipolytic activity of arterial wall in albino rats. II. Effect of magnesium gluconate.


Effects of chelation of calcium on the gastric mucosal barrier.


The isolation and genetic characterization of mutants of the tryptophan system of Bacillus subtilis.


An analysis of arginine requiring mutants in Pseudomonas aeruginosa.


A map order for his I, one of the genetic regions controlling histidine biosynthesis in Pseudomonas aeruginosa, using the transducing phage F116.


The hydrolysis of Tween 80 by vibrios and aeromonads.


Economic aspects of arthritis and rheumatism.


Aspects of the action of glutaraldehyde on Escherichia coli.


The effect of stimuli followed by response-independent shock on shock-avoidance behavior. Four rhesus monkeys were trained on a non-discriminated shock-avoidance schedule (baseline). Stimuli followed by response-independent shock were then presented with the avoidance baseline no longer in effect. The main portion of the experiment consisted of superimposing (independently of responding) the stimuli followed by response-independent shock on the avoidance baseline. Different temporal values of stimulus duration and delay of shock (produced by an avoidance response) were presented successively, using each subject as his own control. When the stimulus duration was short or the delay of shock was long, so that avoidance rate during the stimulus could assume any value without resulting in baseline (avoidable) shocks during the stimulus, a lowered or "suppressed" rate of responding developed during the stimulus. When the stimulus duration was long or the delay of shock was brief, so that avoidable shocks resulted from a response decrement during the stimulus, high or "facilitated" rates of responding developed for a large proportion of the time that the stimulus was present.


Conditioned nalorphine-induced abstinence changes: persistence in post morphine-dependent monkeys. Every tenth lever-press of three morphine-dependent rhesus monkeys was reinforced with food. A red light, initially a neutral stimulus, was presented every third or fourth session for 5 min before and 5 min after an intravenous injection of nalorphine, a morphine antagonist that produces an immediate abstinence syndrome in morphine-dependent monkeys. After several pairings, conditioned suppression of lever pressing, heart-rate decrease, vomiting, and excessive salivation were observed during the red-light period before nalorphine injection. No conditioned electrocardiogram, respiration or temperature changes occurred. After 10 red light-nalorphine pairings, morphine administration was completely discontinued and monkeys were then tested monthly for persistence of the conditioned responses. The red light paired with saline injection continued to suppress lever pressing and to produce heart-rate decreases after 60 to 120 days of complete abstinence from morphine. Subsequently, daily presentations of the red light-saline injection complex rapidly extinguished these conditioned responses. Nevertheless, they could be rapidly reinstated by additional nalorphine injections.


Punishment of elicited aggression.

Punishment of elicited aggression. Aversive shocks are known to produce aggression when the shocks are not dependent on behavior and to suppress behavior when the shocks are arranged as a dependent punisher. These two processes were studied by presenting non-dependent shock to monkeys at regular intervals, thereby producing biting attacks on a pneumatic tube. Immediate shock punishment was stimultaneously delivered for each biting attack. The attacks were found to decrease as a function of increasing punishment intensity. These results show that aggression is eliminated by direct punishment of the aggression even when the stimulus that is used as a punisher otherwise causes the aggression.


Incidence of viable mycobacteria tuberculosis on alginate impressions in patients with positive sputum.


Studies on hog intrinsic factor. II. Immunoradioautographic studies of the properties of hog intrinsic factor and evidences suggesting the possible existence in hog tissues of hog intrinsic factor.


Studies on hog intrinsic factor. 3. Immunologic evidence suggesting the absorption of hog intrinsic factor from human intestine.


Cardiovascular radiation syndrome.

Cardiovascular radiation syndrome.


Evaluation of antimotion sickness drugs: a new effective remedy revealed.


Interferons.

Interferons.


Biochemical studies on Anacystis nidulans during its synchronous growth.


Antimicrobial activity of the leprostatic drug 3-(p-chloranilino)-10-(p-chlorphenyl-2,10-dihydro-2-(isopropylimino)-phenazine (G 30'320, B. 663).


Studies of peptide antibiotics. XV. The synthesis of cyclo-(1-valyl-1-ornithyl-1-leucyl-d-phenylalanyl-1-prolyl-glycyl-glycyl).


Recent developments in the treatment of gonorrhoea. Rising failure rates with single injections of penicillin in gonorrhea are causing alarm.  In the Far East, the failure rate is about 30% after single injections of 2.4 mega units of aqueous procaine penicillin.  In London, although there is rising gonoccoccus resistance to penicillin, single injections of 1.2 mega units of aqueous procaine penicillin nets a cure rate of 85.8% to 91.5% in male patients.  Other recent experiences in the treatment of gonorrhea in London men are outlined in this article.  The failure rate was reduced to 5.8% when a single injection of 2.4 mega units of procaine penicillin was given.  When 1 gm probenecid was given orally along with 1.2 mega units of procaine penicillin, the failure rate was 6.6%.  Streptomycin is so ineffective now against gonorrhea that it is no longer acceptable even as a first treatment in patients who are allergic to penicillin.  In London, the failure rate with streptomycin is 31.7%.  Experts think the rate will jump to 85.8% in the next year.  Many other antibiotics can be used against gonorrhea, but, as in the case of streptomycin, they may lose their effectiveness.  Some recent experiences show that a combination of rolitetracycline by injection and tetracycline by mouth does not give as good results as a single injection of aqueous procaine penicillin but that comparable results can be obtained with  single oral doses of rifampicin and superior results (95.7% success) were obtained with 2 oral doses of dementhylchlortetracycline.


Carnosine and related substances in animal tissues.


New concepts in cancer control--preventable and avoidable cancer.


Experimental acute pancreatitis.

Experimental acute pancreatitis.


The last surviving cancer cell: the chances of killing it.


Changes in the lipolytic activity of the arterial wall in albino rats. II. Effect of ascorbic acid.


Electron microscopy of loosened 50 s ribosomal subunits of Bacillus subtilis.


Detection, diagnostic evaluation, and treatment of dysplasia and early carcinoma of the cervix.


Unproven methods of cancer treatment: H.11.


An epidemiological approach to gastrointestinal cancer.


Vaginal irrigation smear technique for mass screening.


Viewing the journal of the National Cancer Institute.


Fluorides and general health.

Fluorides and general health.


Cell differentiation and pigment composition in Anabaena cylindrica.


Action of hydroxyurea on Anabaena variabilis. II. Effects of pre- and post-treatment with chloramphenicol and base analogues.


Nitroglycerine in angina.

Nitroglycerine in angina.


Relapses in birds infected with species of Leucocytozoon.


Acute brucellosis treated with trimethoprim and sulphamethoxazole. Four patients with acute brucellosis are described, none of whom had any connexion with farming or milk industry, the source of infection being different in each case. The diagnosis was made by serological tests, and in three of the four cases was confirmed by positive cultures from bone marrow (one case) and liver biopsy (two cases). Treatment with the combination of trimethoprim/sulphamethoxazole was successful in three out of four cases, and in the fourth case failure may have been due to the development of trimethoprim resistance.


Chemical modification of tyrosyl residues of the neutral protease obtained from Bacillus subtilis var. amylosacchariticus.


The ankle arthrogram.

The ankle arthrogram.


The test for SH-Australia antigen in viral hepatitis.


Sources of infection with Pseudomonas aeruginosa in patients with tracheostomy.


Antibacterial activity of methenamine hippurate.


Urogenital tuberculosis.

Urogenital tuberculosis.


Medical 6 MeV linear accelerator made in Japan.


Molecular basis of bacterial sporulation.


Immunosuppressive effects of soluble cell membrane fractions, donor blood and serum on renal allograft survival.


RNA polymerase mutants blocked in sporulation.


Structural alteration of RNA polymerase during sporulation.


Effect of 2-(alpha-hydroxybenzyl)-benzimidazole and guanidine on the uncoating of echovirus 12.


Conversion of palmitate to respiratory carbon dioxide by foetal tissue of man and monkey.


Inhibition by drugs of smoking behaviour in monkeys.


"Put away"--for how long?

"Put away"--for how long?


Use of the packaged disaster hospital in Nigeria.


Observations on a matching program to retrain inactive medical technologists.


The changing concept of medical records.


Cost analysis of the developmental phase of an automated multiphasic health testing facility.


Preliminary report on a mass program for detection of gonorrhea.


Procedures used in crisis intervention by suicide prevention agencies.


Epidemiologic studies of otitis media among Eskimo children.


Evaluating the training of nurses to do family planning work in India.


Prevalence of Trichinella spiralis in commerical pork sausage.


An indicator to aid management in assigning program priorities.


Complement fixation test in Trypanosoma rhodesiense infection with cultured Trypanosoma cruzi as antigen.


Interracial marriages in Maryland.

Interracial marriages in Maryland.


Effects of prostaglandins E1 and F1alpha on the heart-lung preparation of the dog.


Carbonic anhydrase polymorphism in cattle and swine.


Radiochemical investigation of the respiration of spores of Bacillus cereus.


Studies with deoxyribonucleic acid from blue-green algae.


On chemolithotrophy and hydrogenase of a gram-positive knallgas bacterium.


Enzymes of Trichomonas foetus. Separation and properties of two beta-galactosidases. The beta-galactosidase activity in extracts of Trichomonas foetus is separable into two fractions by gel filtration on Sephadex G-200. When o-nitrophenyl beta-d-galactoside is used as substrate the first fraction to be eluted, beta-galactosidase 1, has 50 times the activity (units per mg of protein) of the crude preparation. This fraction is activated by Mn(2+) and Co(2+) and inhibited by Hg(2+) and EDTA. In the presence of Mn(2+) the pH optimum for the hydrolysis of o-nitrophenyl beta-d-galactoside or lactose is 5.8-6.0. beta-Galactosidase 1 is an exoglycosidase that releases beta-linked galactose joined to aliphatic and various carbohydrate aglycones. Hydrolysis is prevented, however, by a substituent on either the subterminal sugar or the terminal non-reducing beta-galactosyl residue in an oligosaccharide. The second fraction, beta-galactosidase 2, is not activated by metal ions or inhibited by EDTA and has a broad pH optimum from 4.5 to 6.0.


The effect of ethylenediaminetetra-acetate on Pseudomonas alcaligenes and the composition of the bacterial cell wall. 1. EDTA in borate buffer has a marked bactericidal effect on Pseudomonas alcaligenes, which is more sensitive than Pseudomonas aeruginosa. The bactericidal effect is accompanied by solubilization of lipopolysaccharide and release of intracellular solutes. These effects are more pronounced at pH9.2 than 7.1. 2. Cell walls of P. alcaligenes were prepared and from them were obtained the readily extracted lipids and the fractions given by treatment with aqueous phenol. 3. The cell walls and the above components were analysed and results are compared with those for P. aeruginosa. 4. Lipopolysaccharide obtained by treatment of cell walls with aqueous phenol is contaminated with glycosaminopeptide to a variable extent. 5. The lipopolysaccharide contains less neutral sugar but more phosphorus than the lipopolysaccharide of P. aeruginosa; fucosamine is not a component of the lipopolysaccharide of P. alcaligenes.