Growth during the bacterial cell cycle: analysis of cell size distribution. Cell volume distributions were determined electronically for steady-state cultures of Escherichia coli, Bacillus megaterium, Bacillus subtilis, and Salmonella typhimurium by use of a Coulter transducer-multichannel analyzer system of good resolution. All of the cell volume distributions had the same general shape, even though cultures were grown at widely different rates. Some results were independent of any particular growth model. Both the variability in the volumes of dividing cells and the fraction of constricted and unseparated doublet cells increased with growth rate. The greater separation to single cells at slow growth rates is in agreement with the general finding that filamentous and hyphal forms are greatly reduced in slowly growing chemostat cultures. The distributions were fitted equally well by simple models which assumed that cell growth was either linear or exponential throughout the entire cell cycle. It is concluded that methods of determining growth rate by analysis of distributions of bacterial volumes do not yet have sufficient resolution to distinguish between a variety of alternative models for growth of bacteria.


Methylcellulose effect on cell proliferation and glucose utilization in chemically defined medium in large stationary cultures.


Several control measures used in an epizootic of transmissible gastroenteritis.


Determination of antisera titres ing the single radial immunodiffusion method.


Laser damage thresholds for ocular tissues.


Enzymic cleavage of human gamma-M-globulins and their 8S subunits.


Isolation of haptenic material from ragweed pollen.


Antigenic properties of bovine, porcine, and ovine erythrocyte stroma after solubilizaton by sodium dodecyl sulfate and sonification.


Further studies on the prostatic tissue antigens. Sepation of two molecular forms of aminopeptidase.


Human carbonic anhydrases. II. Some physicochemical properties of native isozymes and of similar isozymes generated in vitro.


The pyruvate formate-lyase system of Streptococcus faecalis. I. Purification and properties of the formate-pyruvate exchange enzyme.


Purification and comparative properties of a pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus.


A long-term nasogastric feeding tube made from modified Penrose tubing.


Differentiation of glomerular, tubular, and normal proteinuria: determinations of urinary excretion of beta-2-macroglobulin, albumin, and total protein. A low molecular weight beta(2)-globulin (beta(2)-microglobulin), albumin, and total protein were measured in concentrated 24-hr urine specimens from 20 healthy subjects and 30 patients with clinical proteinuria of glomerular or tubular type. Classification of proteinuria was made on the basis of clinical diagnosis and size distribution of urinary proteins after gel chromatography. The molecular radii (Stokes' radii) of beta(2)-microglobulin and albumin, estimated by gel chromatography, were 15 A and 35 A. The average 24-hr urinary excretion in healthy subjects was 0.12 mg for beta(2)-microglobulin, 10 mg for albumin, and 80 mg for total protein. The patients with renal glomerular disorders had normal or only somewhat increased excretion of beta(2)-microglobulin, despite considerably increased excretion of albumin and total protein. Most of the patients with tubular dysfunction excreted large amounts of beta(2)-microglobulin, although they excreted normal or only slightly increased amounts of albumin and only moderately increased quantities of total protein. Consequently, the ratio or urinary albumin/urinary beta(2)-microglobulin was high in glomerular proteinuria (1100: 14,200), intermediate in normal proteinuria (33: 163), and low in tubular proteinuria (1.0: 13.3). Determinations of urinary clearances of beta(2)-microglobulin and albumin in four healthy subjects and 11 patients indicated that increased excretions of the two proteins were associated with increased clearances. The results suggest that quantitative determinations of urinary beta(2)-microglobulin and urinary albumin may be useful for detecting disorders of the renal handling of plasma proteins. The findings also seem to suggest a selective tubular reabsorption of the two proteins. Estimates on sera revealed a close correlation between serum levels of beta(2)-microglobulin and creatinine and also a greatly raised serum concentration of beta(2)-microglobulin after bilateral nephrectomy.


A lipoprotein characterizing obstructive jaundice. I. Method for quantitative separation and identification of lipoproteins in jaundiced subjects. Three immunochemically and electrophoretically distinct lipoproteins, LP-A, LP-B, and LP-X,(1) were isolated from the low density lipoprotein fraction (1.006-1.063 g/ml) in plasma from patients with biliary obstruction by a separation procedure which combines ultracentrifugation, heparin precipitation, and ethanol fractionation. This method, here described, permits the quantitative determination of individual plasma lipoprotein families on the basis of their protein moieties, rather than on the basis of their lipid moieties or density. The chemical composition of the unique lipoprotein, LP-X, was similar to that of an abnormal lipoprotein, OLP, isolated by Russ et al. (29) and by Switzer (30). In obstructive jaundice plasma, the combined LP-X and LP-B accounted for 98% and the LP-A for only 2% of the total protein content of the LDL fraction. This study indicates that the plasma lipoprotein pattern in obstructive jaundice is characterized by (a) a decreased concentration of HDL, (b) an increased concentration of LDL, and (c) the presence in the LDL fraction of varying amounts of a specific lipoprotein, LP-X, immunochemically and chemically distinct from LP-A and LP-B. LP-X, with its characteristically high content of unesterified cholesterol and phospholipids, is primarily responsible for the unusual protein and lipid content of the LDL fraction. Screening tests in 61 patients with various forms of jaundice indicated that a characteristic immunoelectrophoretic precipitin are between plasma samples and purified antibodies to LP-X was observed only in patients with obstructive jaundice. This simple immunochemical test may represent a valuable new tool in the differential diagnosis of obstructive and nonobstructive jaundice.


Basement membranes. Synthesis and deposition in response to cellular injury.


Diabetes detection.

Diabetes detection.


The absorption of biliary and non-biliary radiocopper in the rat.


Physical state of bile acids in intestinal contents of germfree and conventional rats.


Chemotherapy of chronic destructive tuberculosis of the lungs and some disputable drugs.


Methods and results in the fight against chronic tuberculosis in Czechoslovakia.


The syndrome of open cavity healing.

The syndrome of open cavity healing.


Chemotherapy of chronic pulmonary tuberculosis with polyresistant bacteria with reference to ethambutol and capreomycin.


A controlled clinical trial of the drug treatment of new cases of pulmonary tuberculosis.


Results of the fight against tuberculosis in the Netherlands.


Biochemical methods for determination of the degree of activity of pulmonary tuberculosis.


Practical aspects of work on tuberculosis in South Korea.


Hurler's syndrome: deficiency of a specific beta galactosidase isoenzyme. A marked deficiency of a specific thermolabile beta-galactosidase isoenzyme (pH optimum 3 to 5) was found in liver and kidney tissues of five patients with the Hurler's syndrome (types 1 to 3).


The sites of deep vascular schizogony in Plasmodium coatneyi malaria.


Further Toxocara skin sensitivity tests in animals experimentally infected with Toxocara canis.


Transovarial development of scrub typhus rickettsiae in a colony of vector mites.


Research into environmental pollution. Report of five WHO scientific groups.


Pesticide residues in food. Report of the 1968 Joint FAO-WHO Meeting.


Formation of gallstones. II.

Formation of gallstones. II.


Chromatographic research on the aqueous humor in a case of gargoylism with corneal dystrophy.


Studies on riboflavin metabolism in the rat. II. Metabolic flavin elimination after oral or intraperitoneal administration of riboflavin-5'-phosphate.


Antibacterial activity in semen and its relation to semen quality.


Single radial immunodiffusion: a method for determination of antiserum titers.


Experimental retinal detachment in the owl monkey. VI. The ERG of the detached and reattached retina.


Agar gel electrophoresis of serum proteins in uremic dogs.


A biochemical study of the pathogenesis of iron-induced myodegeneration of piglets.


Daily rhythms of the activity of aspartate aminotransferase (GOT)-2.6.1.1- and alanine aminotransferase (GPT)-2.6.1.2.- in a homogenate of chicken liver.


Biochemical analysis and seasonal variation of certain cow blood constituents.


Viral valvulitis.

Viral valvulitis.


Effects of acute changes in serum calcium levels on the systolic time intervals in man.


Attenuated rubella viruses. Laboratory and clinical characteristics.


Clinical trials of RA 27-3 (Wistar) rubella vaccine in Japan.


Vaccination of infants with HPV-77 vaccine.


Laboratory testing in rubella vaccine control.


Immunization of man against rubella. Discussion on sessions 3 and IV.


Production of rubella virus vaccine. Live, attenuated in canine renal cell cultures.


Production and testing of rubella virus vaccine. Prepared on WI-38 cell cultures.


Safety testing of rubella virus vaccine (Cendehill strain). Preparation in primary rabbit kidney cells.


Panel discussion on future of rubella virus vaccines.


Plasma diamine oxidase activity in pregnancy: a reappraisal.


Changes in the cardiac output and uterine blood flow of the pregnant Macaca mulatta.


Injectable steroids in allergy.

Injectable steroids in allergy.


Complications of cervical esophagostomy.


Snake antivenin. Effect on survival time in experimentally induced hemorrhagic pancreatitis.


Prevention of invasive pseudomonas infection in burns with a new vaccine.


Representation of central visual fields in prestriate cortex of monkey.


Phylogenetic changes in the proportions of two kinds of lactate dehydrogenase in primate brain regions.


Combined chemotherapy in the treatment of malignant effusions.


Observations on the extrinsic neural control of cerebral blood flow in the baboon.


Interference between viruses of the myxogroup in primary monolayer calf kidney cell cultures.


Different numbers of oligosaccharide units in glycoproteins of the same immunological class and type.


Cellular kinetics associated with the development of acquired cellular resistance. Mice infected with either BCG or Listeria monocytogenes display the same type of cellular response pattern. In both infections there is an intense proliferation of splenic lymphoid cells, a coincident proliferation of differentiated macrophages, and a subsequent production of macrophages with increased metabolic potential. The temporal relations between these events and the onset of host resistance indicate that lymphoid cell proliferation and macrophage proliferation are essential events in the response which leads to cellular resistance against facultative intracellular parasites.


The mitotic potential of fixed phagocytes in the liver as revealed during the development of cellular immunity. Fixed macrophages in the liver of the mouse undergo mitosis in large numbers during an infection with Listeria monocytogenes. The macrophage mitotic response always precedes the expression of efficient host immunity to infection. It is suggested that macrophage proliferation is an important event in the development of host immunity to L. monocytogenes.


Immunity to mumps in an institutional epidemic. Correlation of insusceptibility to mumps with serum plaque neutralizing and hemagglutination-inhibiting antibodies.


Electrocardiographic patterns in experimental cerebral concussion.


Renewal of oviduct cilia during the menstrual cycle of the rhesus monkey.


Marburg agent disease: in monkeys.

Marburg agent disease: in monkeys.


Termination of pregnancy in the rat with rabbit antibovine LH serum.


Marburg agent disease: pathology.

Marburg agent disease: pathology.


Increased virulence of Plasmodium cynomolgi bastianellii in the rhesus monkey.


Survival of the Trypanosoma cruzi in the medicinal leech (Hirudo medicinalis).


Bacteria isolated from the gut of some Reduviid bugs.


A relation between hysteresis and other visco elastic properties of some biomaterials.


The in vitro lysis of the red cells of paroxysmal nocturnal haemoglobinuria by the serum of patients with cirrhosis of the liver.


The distribution of folates in rabbit tissues during phenylhydrazine-induced haemolysis.


Post-primary flash-evoked responses in unanaesthetized night- and day-active monkeys.


Bilateral stress fractures of the naviculars. A case report.


The effect of pentobarbital anesthesia on resuscitation and brain damage in fetal rhesus monkeys asphyxiated on delivery.


Iridocyclitis in juvenile rheumatoid arthritis.


Abortion in sheep associated with Brucella abortus infection.


Pseudomonas aeruginosa contamination of a warm water system used for pre-milking udder washing.


Blood parasites found in imported birds at post-mortem examination.


Activity of chlorinated lincomycin analogues against Plasmodium cynomolgi in rhesus monkeys.


Treatment of trophozoite-induced Plasmodium knowlesi infection in the rhesus monkey with Trimethoprim and sulfalene.


Cross-reactivity in fluorescence tests for Trypanosoma and Leishmania antibodies. A simple inhibition procedure to ensure specific results.


Hemagglutination-inhibition test for the evaluation of the antigenicity of ascarid fractions.


Experimental trachoma in owl monkeys.

Experimental trachoma in owl monkeys.


Dengue type 3 virus isolated from an antiguan patient during the 1963-64 Caribbean epidemic.


Nasal and tracheal resistance of swine against reinfection by Bordetella bronchiseptica.


The effect of antilymphocyte serum (ALS) on the secondary immune response in mice.


The role of chloride ion in photosynthesis. 3. The effect of artificial electron donors upon electron transport.


Nucleoside antibiotics. IV. Metabolic fate of adenosine and cordycepin by Cordyceps militaris during cordycepin biosynthesis.


5-fluoropyrimidin-2-one deoxyriboside and its growth-inhibiting properties.


Isolation of polyribosomes and characteristics of an amino acid-incorporation system from the liver of a primate (Papio papio).


Preparation and properties of cyanoethylated insulin. An insulin derivative with blocked amino- and imidazole-groups.


The mechanism of laccase-catalyzed oxidations: kinetic evidence for the involvement of several electron-accepting sites in the enzyme.


Immunochemical studies of isolated human brain ganglioside components.


Quantification of individual CSF proteins by immune precipitation in agar gel.


Total intravenous alimentation.

Total intravenous alimentation.


Bacteria in the chronic ear. Pre- and postoperative evaluation.


Evaluation of activity in tuberculous cavities of the lung.


Transplantation of the heart and both lungs. I. Historical review.


Post-irradiation surgery for bronchogenic carcinoma.


Permissible levels of occupational exposure to airborne toxic substances.


Separation of immunologically active fragments by membrane partition chromatography.


Regulation of malate dehydrogenase in the liver of newborn rabbits maintained by a convenientew technique of artificial feeding.


Studies on the oxygen uptake of leucocytes from the Chacma baboon(Papio ursinus) and on a new medium for the in vitro study of intact leucocytes.


The effects of Minchinia nelsoni infection on enzyme levels in Crassostrea virginica. II. Serum phosphohexose isomerase.


Species differences in baboon milk lipase (tributyrinase) and esterase activities.


AMP-deaminases from avian brain and muscle: catalytic and immunological differences.


Metallic ions and habituation in the protozoan Spirostomum.


The hemolymph proteins of the blue crab, Callinectes sapidus. II. A lipoprotein serologically identical to oocyte lipovitellin.


Allantoate amidohydrolase. I. pH- and anion-dependent activation.


Allantoate amidohydrolase. II. Inactivation and instability.


Studies of the electrical parameters and behavioral effects of an EEG "seizure discharge".


The effect of testosterone or oestradiol upon the sexual and associated behaviour of the adult female Rhesus monkey.


Recording myometrial activity in freely-moving Rhesus monkeys by telemetry.


The control mechanism of sclerotial formation in Sclerotium rolfsii Sacc.


Spore germination mutants of Bacillus cereus.


Kinetic parameters of mesophilic and thermophilic isocitrate lyase.


Antigen-antibody reactions in agar. 8. A micro-modification of the Oudin agar gel precipitin method for the determination of antibody concentrations in absolute weight units.


Evolution of medical service plans.

Evolution of medical service plans.


Excretion of accessory genital glands-specific antigens in the urine of healthy male rats.


Abrasive ablation: a new experimental surgical technique to study nonparenchymal aspects of hepatic regeneration.


Human malaria (Plasmodium falciparum) in owl monkeys (Aotus trivirgatus).


Gastric mucosal antibodies and iron absorption.


The role of corticoids in the management of shock.


Studies on in vitro replication of Bacillus subtilis DNA.


On the regulation of the initiation of DNA replication in bacteria.


Comparison of perfusion and non-perfusion of preserved baboon kidneys with and without oxygen.


Immunofluorescent localisation of calcitonin in medullary C-cell thyroid carcinoma, using antibody to the pure porcine hormone.


Planning for community health.

Planning for community health.


Fabry's disease in children. An electron microscopic study.


Maintenance therapy of pernicious anaemia. Comparison of cyanocobalamin, hydroxocobalamin and a cyanocobalamin-zinc-tannate complex.


DNA synthesis by erythroblasts in normal man and in disorders of erythropoesis.


Change from fetal to adult hemoglobin in relation to changes in the A antigen of human erythrocytes.


Congenital deficiency of fibrin-stabilizing factor (factor XIII). A report of the first case from Latin America.


Effect of reticulo-endothelial blockade on experimental murine infections.


Hyaluronic acid capsule in a Streptococcus faecalis var. zymogenes. A comparison with related mucoid strains.


Radioactive depot-corticosteroid penetration into monkey ocular tissue. II. Subconjunctival administration.


Specificity of binding subsites of allantoicase.


Distribution and immunochemical properties of rat kidney L-amino-acid oxidase, with a note on peroxisomes.


Characterization of sodium borohydride-reduced histaminase-histamine intermediate.


The variations in the antigenic composition of Mycobacterium tuberculosis during the growth cycle as measured by the passive hemagglutination and precipitation reactions.


Carbohydrate metabolism in Pseudomonas aeruginosa: a procedure for accumulating phosphorylated intermediates.


Characterization of a protein-lipopolysaccharide complex released from cell walls of Pseudomonas aeruginosa by ethylenediaminetetraacetic acid.


Chromosomal regions which control sporulation in Bacillus subtilis.


The chemical inhibition of an enzyme induced germination of bacterial spores.


Lead, lithium and manic-depressive psychosis.


Structure-function relationships for some metalloalkaline phosphatases of E. coli.


Cerebrospinal fluid production during temperature stress and feeding in the conscious monkey.


Suppressed multiplication of pulmonary tubercle bacilli in bacillary trypsin extract-immunized mice.


A review of pharmacy for nurses.

A review of pharmacy for nurses.


Alteration of a learned response of the squirrel monkey by hallucinogens.


Aqueous flow and entry of cycloleucine into the aqueous humor of vervet monkeys (Cercopithecus ethiops).


Influence of intraocular pressure and some drugs on aqueous flow and entry of cycloleucine into the aqueous humor of vervet monkeys (cercopithecus ethiops).


Hemodynamics of staphylococcal B enterotoxemia and other types of shock in monkeys.


Partition data of penicillins determined by means of reversed-phase thin-layer chromatography.


Studies of substrate regulation in fasting. II. Effect of infusion of glucose io the carotid artery upon fasting lipolysis in the baboon. The effect of glucose infusion on rates of lipolysis were studied in a group of chair-trained papio baboons that had been prepared for chronic intravenous and intracarotid infusion. All studies were carried out after a 24 hr period of fasting and when the animals were fully awake. After a control interval of 1 hr, a glucose infusion was begun either intravenously or intra-arterially. The infusion was continued at a constant rate for 2 hr and then changed directly to the alternate route and continued an additional 2 hr. Blood samples were collected at 30-min intervals for glucose, free fatty acid (FFA), glycerol, insulin, and in some studies, growth hormone (GH) determination. When glucose doses less than 0.5 mg/kg per min were used, no change in the products of lipolysis was noted during either venous or carotid administration, and glucose and insulin levels remained stable or fell gradually. With doses of glucose between 0.5 and 0.6 mg/kg per min, a greater fall in both FFA and glycerol was noted during carotid administration. No definite changes in plasma glucose or insulin levels were noted during either infusion period. These changes in lipolysis were noted regardless of the sequence of infusion, and a similar differential suppression of FFA was noted during a 24 hr period of carotid glucose administration. When doses of glucose larger than 0.6 mg/kg per min were used, inhibition of lipolysis was noted during both phases of infusion. No definite change in GH levels was noted during the periods of fasting, and the levels of the hormone did not appear to be related to changes in glucose, insulin, or FFA levels. These data provide additional evidence for the presence in the central nervous system of a glucose-sensitive center which alters lipolytic rates independently of insulin and GH, probably by altering sympathetic tone to adipose tissue.


Radioimmunoassay of beta lipoprotein-protein of rat serum. A double antibody radioimmunoassay for rat serum beta lipoprotein-protein (beta Lp-protein) is described. The protein was purified by ultracentrifugation, selective heparin-manganous precipitation, and gel filtration on Sephadex G-200. Antiserum was prepared in rabbits by biweekly immunization and absorbed with nonbeta lipoprotein containing rat serum. Iodination with (125)I and purification by gel filtration provided a radiolabeled protein which was > 98% displaced by purified beta lipoprotein in the immunoassay. The radioimmunoassay was sensitive to beta Lp-protein concentrations from 0.1 to 1.5 mug. Specificity of the immunoassay for beta Lp-protein was established by comparison of the displacement curves obtained with serum very low density lipoprotein (VLDL), low density lipoprotein (LDL), high density lipoprotein (HDL), and density (d) > 1.21 fractions and with the beta and alpha migrating lipoproteins eluted from paper electrophoretograms. Suitability of the assay for measuring beta Lp-protein in serum was established by demonstrating 100% recovery of beta lipoprotein added to whole serum and by the absence of immunoreactive beta Lp-protein in serum of orotic acid-treated rats. Examination of sera from six other vertebrates species revealed partial cross-reactivity. Normal rat serum was found to contain 0.25+/-0.01 mg/ml of beta Lp-protein and hepatic production by an isolated perfused rat liver system was determined as 0.145 mg/hr.


The dependence of the oxygen-concentrating mechanism of the teleost eye (Salmo gairdneri) on the enzyme carbonic anhydrase. Microoxygen polarographic electrodes were constructed and used to measure oxygen tension (P(OO2)) in the eyes of rainbow trout (Salmo gairdneri). The values obtained are compared with arterial blood and environmental water P(OO2) and indicate that there is an oxygen-concentrating mechanism in the eye supplying oxygen to the avascular retina. Anatomically similar retes suggest that the mechanism is similar to the one which exists in the swim bladder. Elimination of the arterial blood supply to the choroidal gland rete mirabile of the eye (through pseudobranchectomy) and the consequent lowering of ocular oxygen tensions implicate the choroidal gland as one of the major components of the oxygen-concentrating mechanism. After pseudobranchectomy the presence of ocular P(OO2) above that of arterial blood is indicative of a secondary structure in the eye capable of concentrating oxygen. Inhibition of carbonic anhydrase, using acetazolamide, is shown to result in complete suppression of the oxygen-concentrating mechanism. A hypothesis is advanced for the participation of retinal-choroidal and erythrocyte carbonic anhydrase in the oxygen-concentrating mechanism.


Determination of calcium in pharmaceutical preparations by atomic absorption spectrometry.


Interaction of trachoma elementary bodies with host cells.


The yellow colour of the lens of man and other primates. 1. Measurements have been made of the absorption spectra of the lenses of human, baboon (Papio), rhesus monkey (Macaca), squirrel monkey (Saimiri sciureus) and bush baby (Galago crassicaudatus).2. In all these species an absorption maximum was found between 365 and 368 nm.3. The pigment responsible for this absorption was water-soluble and aqueous extracts were examined. Protein-free aqueous extracts showed an additional maximum at 260 nm which could be only partially accounted for by the presence of ascorbic acid.4. Chromatography of the protein-free solution from human lenses yielded a fast-moving yellow component with a blue fluorescence. Its absorption spectrum was very similar to that of the original protein-free solution. A fast-moving yellow component from the baboon lens had a yellow fluorescence.5. The human lenses appeared to contain more of the yellow, water-soluble pigment at birth than in adult life. The concentration remains constant during adult life.6. There is evidence for the appearance of another pigment in the human lens in later adult life. It is not water-soluble and has an absorption maximum at about 330 nm.


Transplacental infection of the mouse fetus by Coxsackie viruses.


Congenital pseudarthrosis of the ulna. Report of two cases.


The release of thyroxine from serum protein in the vessels of the liver. 1. An examination has been made of the uptake of [(131)I]L-thyroxine and L-3,5,3'-tri-iodothyronine by the perfused rat liver.2. With no serum in the perfusion fluid and at flow rates less than 1.5 ml./g.min the liver removes about 85-90% of the hormone passing through in the perfusion fluid. At higher flow rates, the proportion of the hormone taken up is reduced and the results suggest that half of the hormone would be removed if the perfusion fluid spent about 1 sec in the liver.3. The percentage of the hormone extracted by the liver is not affected by variations in temperature (between 22 and 37 degrees C) or by variations in thyroxine concentration (between 1 x 10(-4) and 1 x 10(-1) mug/ml.).4. Accumulated radioactive thyroxine is released only slowly into the perfusion fluid. The rate is doubled by perfusion with solutions containing 10% serum.5. With serum present in the perfusion fluid the uptake of thyroxine can be resolved into two distinct components. A small proportion is taken up very rapidly and a large proportion very slowly. It is suggested that the rapid process represents uptake of free thyroxine and the slow process uptake of thyroxine released from the serum protein; the half-time of the slow process being a measure of the rate of release.6. The proportion of thyroxine taken up by the fast process is about 9% in solutions containing 1% serum and falls progressively at higher serum concentrations.7. With tri-iodothyronine the proportion of the hormone in the free state and the rate of uptake by the slow process are much greater than with thyroxine.8. In the presence of high concentrations of thyroxine the proportion of the hormone in the free state and the rate of uptake by the slow process are both increased.9. The rate of release of thyroxine from its binding protein is proportional to the percentage of the hormone in the free state.


The facile isolation of a structural phospholipoprotein from hydrogenomon as facilis and Neurospora crassa. The paper describes a new and gentle procedure for isolating "structural" phospholipoproteins from organisms and the first such isolation from a procaryotic microbe, Hydrogenomonas facilis. The amino acid composition of its protein moiety resembles that of "structural protein" from other sources. Although disc gel electrophoresis has shown the protein to be heterogeneous, this is attributed to aggregation.


Inactivation of transforming DNA by ultraviolet irradiation: a study with ultraviot-sensitive mutants of Bacillus subtilis.


A template-selective inhibitor of in vitro transcription. A macromolecular factor, TF1, has been isolated from bacteriophage SP01-infected B. subtilis. TF1 selectively inhibits in vitro transcription of SP01 and related viral DNA. Evidence is presented regarding these properties of the repressor-like TF1: (1) template and conformational specificity; (2) interaction with DNA rather than RNA polymerase; (3) trypsin sensitivity; (4) reversibility of action; and (5) ability to block initiation, but not propagation, of RNA synthesis.


Passive hemolysis by serum and cobra venom factor: a new mechanism inducing membrane damage by complement. The studies presented here indicate that activation of the complement (C') system by a foreign protein will cause membrane injury and passive lysis of unsensitized erythrocytes present at the time of the reaction. These observations suggest that in addition to the classical antibody-C'-induced cytolysis, there are alternative pathways or mechanisms for activation and participation of the terminal C' components in the production of cell membrane injury. We have shown that a substance derived from cobra venom and eluted from a single protein band on polyacrylamide can promote lysis of unsensitized autologous or heterologous erythrocytes in the presence of fresh guinea pig serum and that this lysis-inducing activity and C'-inhibiting activity appear to reside in the same fractions. The lytic activity is prevented by several agents known to impair classical C'3 activity, but is unaffected by certain procedures which interfere with the function of C' components C'1 and C'2, a suggestion that this reaction involves chiefly C'3-C'9. Further, the cobra venom (CV) factor depletes C' activity in cobra serum, and the CV factor (with its 5S serum cofactor) converts purified C'3 to its inactive form,(1) indicating that the reaction of this complex with the complement system occurs without participation of antibody. Therefore, since the lysis-inducing and C'-inhibiting activity of the CV factor appear to result from similar interactions with the complement system, these observations suggest that cell membrane damage and cell lysis can be accomplished through activation of the complement system by a mechanism involving little or no participation of classical antibody or C' components C'1, 4, or 2.


The fate of foreign compounds in man and animals.


Studies on the ultrastructure of pseudopod formation in human blood platelets. I. Effect of temperature, period of incubation, anticoagulants and mechanical forces.


Aggregation of human platelets by bovine platelet fibrinogen.


Hepatitis in marmosets: induction of disease with coded specimens from a human volunteer study. Marmosets inoculated with plasma from three early acute hepatitis patients developed hepatitis 30 to 40 days later. Other groups of marmosets receiving preinfection plasmas from the same patients showed no evidence of hepatitis in this experiment. It is, therefore, most probable that hepatitis in marmosets represented transmission of human disease rather than activation of latent "marmoset hepatitis."


Mechanisms of pancreatic exocrine secretion.


Severe regional enteritis with a unique duodenosigmoid fistula following normal pregnancy.


The generation time of tubercle bacilli in vivo after aerogenic infection of the guinea pig.


An enzyme system of Mycobacterium tuberculosis that reacts specifically with isoniazid. II. Correlation of this reaction with the binding and metabolism of isoniazid.


Studies of a biochemical lesion in experimental tuberculosis in mice. XI. Mitochondrial swelling induced by cord factor in vitro.


Radioactive content of Mycobacterium tuberculosis after exposure to 14C-isoniazid.


Segmental replacement of the common bile duct.


Proprioception and vibration sensibility discrimination in the absence of the posterior columns.


Acid-base changes during lidocaine induced seizures in Macaca mulatta.


Studies on a filtrable agent associated with infectious enteritis (bluecomb) of turkeys.


Inhibition of focal seizures by moderate hypothermia. A clinical and experimental study.


Metaboli of radionuclides in workers.

Metaboli of radionuclides in workers.


Comparative metabolism of selected radionuclides in animals.


A system for the study of urea synthesis in liver homogenates.


General hospitals and the long-term care gap.


Effect of various drugs on the intestinal absorption of para-amino-hippurate in rabbits.


Accredited long-term care facilities.

Accredited long-term care facilities.


Diverticulum near the thoracic inlet.

Diverticulum near the thoracic inlet.


Communications. "In vitro" cell reactions in delayed hypersensitivity.


Specific antisera to C1s: detection of different electrophoretic species of C1s.


Development of human complement system.

Development of human complement system.


Identification of an antigen and specific antibody in the sera of chickens infected with Plasmodium gallinaceum.


Ultrastructural and other observations which suggest suctorian affinities for the taxonomically enigmatic ciliate Cyathodinium.


Gamma irradiation of Stentor polymorphus.


Localization of arylsulphatase in neurons.


Ultraviolet-microbeam irradiation of regenerating Stentor.


Mating type inheritance in Glaucoma.

Mating type inheritance in Glaucoma.


Sarcocystis nesbitti n. sp. from the rhesus monkey.


Preliminary report: the effect of environmental factors on electrolyte and acid-base status during isolated bloodless kidney perfusions.


Skin transplantation: orientation of epithelial cells by the basement membrane.


Selection of patients for long-term hemodialysis.


Blood parasites of some pacific northwest amphibians.


Apparent favorable response of lead poisoning in a duck to treatment with a chelating agent.


Separate autoimmune mechanisms for 7s and 19s globulins in Laennec's cirrhosis.


Studies on the microsomal acylation of L-glycerol-3-phosphate. II. The specificity and properties of the rat liver enzyme.


Some factors influencing the urinary excretion of oxalic acid in man.


Technical pitfalls leading to errors in the quantitation of plasma amino acids.


Long term ambulatory treatment of tuberculosis in Hong Kong.


Development of a modified virus strain and vaccine for equine viral arteritis.


The immunologic properties associated with equine infectious anemia: recent findings.


Equine infectious anemia: reports of progress in research.


Electron microscopic characterization of African horse-sickness virus.


Antigenic classification of group C animal streptococci.


Corynebacterial infections in the horse: problems of prevention.


The significance and treatment of haemarthrosis of the knee following trauma.


Chemical weapons on the home front.

Chemical weapons on the home front.


Pathophysiology of parkinsonian tremor.

Pathophysiology of parkinsonian tremor.


Long-term nursing care of an unconscious patient.


The factory is a battlefield.

The factory is a battlefield.


Some thoughts on quality in nursing service.


Nursing organisations.

Nursing organisations.


Slow, latent and temperate virus infections of the central nervous system.


Scrapie--a virus-induced chronic encephalopathy of sheep.


Hemodynamic studies in portal hypertension.


Inhibitory activity of L-asparaginase from Mycobacterium tuberculosis on Yoshida ascites sarcoma in rats.


Purification and properties of UDPG dehydrogenase from beef liver.


The compound active site of Bacillus subtilis neutral protease: some properties of six subsites.


Antigenicity and chemical composition of an enzymatic digest of elastin.


Cystic brain alteration after incomplete placental abruption in monkey.


Neurological manifestations of general xanthomatosis. Hand-Schuller-Christian disease.


A site of reversible conformational alteration in rat liver ribosomes.


Effect of the magnesium concentration in the extracting medium on the polyribosome content and the cell-free amino acid incorporating ability of extracts of logarithmic phase cells of a Bacillus sp.


Specificity of the "in vitro" toxigenicity test for Corynebacterium diphtheriae.


Congenital Chagas' disease. II. Pathological findings in nine cases.


Erythrocyte hypoplasia due to lead poisoning: a devastating, yet curable disease.


Antiplasmodial precipitins demonstrated by double diffusion in agar gel in the serum of rats infected with Plasmodium berghei.


Congenital Chagas' disease. I. Clinical and epidemiological study of 30 cases.


Fluorescent antibody staining of four Babesia species.


Pathogenetic factors in experimental bovine oesophagostomosis. 3. Demonstration of protein-losing enteropathy with 51-Cr-albumin.


Babesia argentina: serum changes in infected calves.


Pathogenesis of experimental cholera. Preparation and isolation of choleragen and choleragenoid. Choleragen, a diarrheagenic protein enterotoxin elaborated by Vibrio cholerae, has been isolated from the supernate of fermenter cultures by steps involving ammonium sulfate precipitation, DEAE cellulose, Sephadex G-75, and Agarose A-5m chromatography. The resulting product appears to be pure according to immunoelectrophoretic, disc electrophoretic, ultracentrifugal, and immunologic criteria. Sephadex gel filtration and membrane filtration studies suggest a molecular size of 61,000. The isolated product is highly active in inducing experimental cholera in infant and adult rabbit models. It also elicits, in small dosage, an increased vascular permeability in skin. These observations indicate that choleragenicity and increased vascular permeability are intimately associated phenomena and may be manifestations of the same basic mechanism. An additional, antigenically identical, protein has also been isolated by the same procedures. The latter substance, termed "choleragenoid", lacks the permeability effect and choleragenicity of the choleragen moiety. Its size (estimated from Sephadex gel filtration at 42,000) is smaller than that of choleragen and it also differs in charge. Choleragenoid may prove useful as a nontoxic immunogen to protect against pathologic effects of V. cholerae infection.


Characterization of lipoprotein particles isolated from the Golgi apparatus of rat liver. It has been proposed that particles within tubules and vesicles of the Golgi apparatus of liver cells are precursors of very low density lipoproteins in blood plasma. To characterize these particles we isolated a cell fraction rich in Golgi apparatus and associated particles from rat liver in quantities sufficient for analysis. Particles freed from the membranes of the Golgi apparatus and floated at d = 1.006 were studied by chemical analysis, immunodiffusion, and paper electrophoresis. The lipid composition of the Golgi particles was similar to that of very low density lipoproteins from the same rats. The protein content was about 10% of dry weight for both the Golgi particles and plasma very low density lipoproteins. The Golgi particles formed lines of identity with plasma very low density lipoproteins during immunodiffusion against antiserum to plasma very low density lipoproteins. On paper electrophoresis, however, many Golgi particles remained near the origin, with only a few migrating to the pre-beta position. It was concluded that the lipoproteins in the Golgi apparatus are the precursors of plasma very low density lipoproteins.


Rare earth nuclides as potential agents for skeletal imaging.


The female sex cycle.

The female sex cycle.


Candida albicans antigens studied in complement fixation test and agar gel diffusion test.


The delivery and financing of child health services.


Enzyme activity of donor origin in mouse radiation chimeras.


An improved method for the direct immunofluorescent identification of Neisseria gonorrhoeae.


Treatment of diabetic ketoacidosis with insulin. How much? When?


Oncogenic simian adenoviruses. V. Recovery of infectious virus from intracranial tumor cells induced by simian adenovirus 7.


A simplified method for estimating free-thyroxine fraction in serum.


Experimental atherosclerosis and the periodontium.


Studies on the biology of the periodontium of marmosets. VI. Arteriosclerotic changes in the blood vessels of the periodontium.


Nutritional intravenous feeding.

Nutritional intravenous feeding.


Metabolic significance of pancreatic ribonuclease.


Metabolic effects of insulin in acute insulin insufficiency.


Hepatitis and Australia antigen: autosomal recessive inheritance of susceptibility to infection in humans. Examples of inherited susceptibility to infection controlled by genes segregating at one or a small number of loci have been identified in lower animals. In this study we report data on what appears to be a similar situation in humans. "Australia antigen" is an antigen found in the sera of patients with acute and chronic hepatitis, and it may actually be a form of virus. It is very common in many tropical areas, and people in these areas having the antigen appear to be hepatitis carriers. The antigen is detected by immuno-diffusion in agar gel (Ouchterlony method). Individuals with the antigen are designated Au(1) and those without it Au(0). Family studies involving 1797 different individuals residing on the island of Bougainville are consistent with the hypothesis that susceptibility to chronic infection with the "antigen" is controlled by an autosomal recessive gene (Au(1)). This confirms the conclusions previously arrived at from similar (but less extensive) studies on the island of Cebu. Individuals with this inherited susceptibility do not ordinarily have overt manifestations of hepatitis. The sign test was used to determine family clustering. Segregation analysis was performed by the method of C. A. B. Smith. In the 41 Au(0) x Au(0) matings, 53.8 recessives were expected in the offspring and 56 were seen (0.7 > p > 0.5). In the Au(1) x Au(0) matings, 40.1 recessives were expected and 42 were seen (0.7 > p > 0.5). The Au(0) x Au(0) matings were also analyzed by the method of Li and Mantel, in which the recessive ratio of 0.25 is expected by the genetic hypothesis. The values observed were 0.2527 for the Bougainville study and 0.2461 for the Cebu study.


Antibodies to acetylcholinesterase.

Antibodies to acetylcholinesterase. A rabbit antiserum against commercially available Electrophorus electricus acetylcholinesterase has been prepared. Five precipitation bands were distinguished by immunoelectrophoresis, but only three of these contained demonstrable enzyme activity. In an in vitro system, activity of the commercial enzyme or of highly purified acetylcholinesterase was inhibited by 70-82 per cent after incubation with antiserum. Antibody specificity was demonstrated by the absence of serological cross reactions or enzyme inhibition with bovine erythrocyte acetylcholinesterase or horse serum cholinesterase.


Amniotic fluid spectral analysis in the management of patients with rhesus sensitization.


Irradiation of an inducible enzyme system in neurospora. UCLA-12-686.


Temperature control of unsaturated fatty acid biosynthesis in bacilli. UCLA-12-686.


Early effects of 150-MEV proton irradiation in rhesus monkeys. SAM-TR-68-87.


Early hematologic changes in the rhesus monkey after superlethal mixed neutron-gamma irradiation. SAM-TR-68-45.


Plate assay methods for amino acids. 7. Tyrosine.


Does America need a new health system?

Does America need a new health system?


Familial hypo-beta-lipoproteinemia: a genetic disorder of lipid metabolism with nervous system involvement.


Hetero analogs of sesquifulvalene.

Hetero analogs of sesquifulvalene.


The Stokes-Adams syndrome.

The Stokes-Adams syndrome.


Topochemical investigations of peptide systems.


Research in hospital services and delivery of care: new frontiers for study.


Crystalline tetrazolinyl radical.

Crystalline tetrazolinyl radical.


Rumen microbial ecology in mule deer.

Rumen microbial ecology in mule deer. Mule deer rumen microbial populations from animals in the natural habitat in Utah and from captive deer fed various rations were studied. The microorganisms were characterized on the basis of morphology and Gram reaction. Rumen samples contained 13 identifiable types of bacteria and one genus of ciliate protozoa (Entodinium). Highest rumen bacterial populations were produced on rations containing barley. No differences in proportions of ruminal bacteria in the various morphological groups could be detected when animals were fed either natural browse plants or alfalfa hay. The total numbers of bacteria were similar for animals feeding on controlled diets of browse or hay and those in the natural habitat. Numbers of some bacterial types were directly related to ciliate protozoal numbers, whereas others were inversely related. Highest rumen ciliate protozoal populations were observed on rations containing barley. No differences in protozoal populations were noted between diets containing only browse or hay. Seasonal variations were noted in ciliate protozoal numbers from deer feeding in the natural habitat. The total number of ciliate protozoa decreased in the fall and winter and remained low until spring. There were indications that salt in the deer diet favorably affected rumen ciliate protozoa. Rather than revealing direct deer management applications, this study serves to stimulate and illuminate new approaches to research in range and wildlife nutrition.


Antimycobacterial activity of rifampin under in vitro and simulated in vivo conditions. Minimal inhibitory concentrations of rifampin for different species of mycobacteria were determined in 7H-10 agar medium and Lowenstein-Jensen egg medium. When rifampin was incorporated into egg medium, approximately 90% of its activity was lost. The stability of rifampin was tested during storage at different temperatures and concentrations. When tested in agar medium, a combination of isoniazid (INH) and rifampin inhibited multiple drug-resistant strains of Mycobacterium intracellulare, but under simulated in vivo conditions the drugs did not eliminate these same organisms. Drug-resistant mutants of M. intracellulare multiplied during an 8-day period when exposed 10 hr daily to the INH-rifampin regimen. However, combinations of rifampin and INH reduced drug-resistant mycobacterial populations by 99%, an effect which could not be enhanced by the addition of either erythromycin, ethionamide, or cycloserine.


Combined action of carbenicillin and gentamicin on Pseudomonas aeruginosa in vitro. The minimal inhibitory and minimal bactericidal concentrations of carbenicillin and gentamicin were determined for 10 strains of Pseudomonas aeruginosa isolated from the urinary tract. Combinations of the two drugs were tested for possible enhanced activity. Such enhancement was demonstrated in the inhibitory activity of combinations for eight strains. Striking bactericidal activity against five strains was achieved by the combination, whereas neither drug alone in low dosage was capable of bactericidal action. The possible mode of action and the possible merit of pursuing these preliminary findings are discussed.


Methods for storing oxidase test reagents. Oxidase test reagents are notoriously unstable, but they can be held ready for use if frozen or if reagent-impregnated filter paper is kept dry.


Induction of spheroplasts of Pseudomonas aeruginosa by carbenicillin. Speroplasts of Pseudomonas aeruginosa were induced with carbenicillin. Morphological changes were observed as early as 1.5 hr after the initial exposure to carbenicillin.


Induction of labour by amniotomy and micro-infusion of oxytocin (Syntocinon).


Comparative trials with different decontaminating agents for growing Mycobacterium tuberculosis from sputum specimens. A reliable culture technique for mycobacteria to enable precise taxonomic and biological identification to be made, e.g., virulence and sensitivity or resistance to the major and minor antituberculosis drugs, is one of the basic requirements of efficient and economic tuberculosis control measures. Several of the methods employed for this purpose have been studied systematically. Many of these methods make use of various detergents, especially quaternary ammonium bases in different combinations, which possess strong bacteriostatic or bacteriocidal properties and decontaminate sputum samples without, however, affecting the viability of mycobacteria.A series of experiments to study the effect of sodium lauryl sulfate and of pancreatin, both in combination with sodium hydroxide, and of a new detergent, di-isobutal-naphthalin-sulfonate (Nekal Bx) on the growth of mycobacteria in liquid and solid media were carried out. As a control technique, the processing of samples of pathological material with hydrochloric acid and sodium hydroxide was studied.On the basis of these experiments, the sodium lauryl sulfate method proved to be promising, both for the rapid growth and characterization of the colonies, and for producing the lowest contamination rate in cultures. However, none of the 4 techniques was sensitive enough under the experimental conditions to demonstrate mycobacteria in sputum samples containing few organisms.


The Second International Standard for anti-Brucella abortus serum. In 1965, the WHO Expert Committee on Biological Standardization (1966) noted that stocks of the International Standard for Anti-Brucella abortus Serum were almost exhausted and requested the Central Veterinary Laboratory, Weybridge, England, to examine a quantity of serum offered by that laboratory for its suitability to serve as a replacement, and to arrange a collaborative assay. After being distributed into ampoules and freeze-dried, the serum was assayed against the International Standard by 17 laboratories in 12 countries. On the basis of the results obtained, the serum has been established as the Second International Standard for Anti-Brucella abortus Serum and the International Unit has been defined as the activity contained in 0.09552 mg of the Second International Standard.


A comparison of some biological characteristics of the mouse-passaged scrapie agents, 22A and ME7.


Reconstruction of lateral ligament tears of the ankle. An experimental study and clinical evaluation of seven patients treated by a new modification of the Elmslie procedure.


Current status of the immunology of malaria and of the antigenic analysis of plasmodia. A five-year review. The immunology of malaria has been intensively studied, and many reviews of separate topics have appeared. Among host factors contributing to susceptibility to malaria, the following are studied in the present paper: (1) genetic factors affecting susceptibility to human and rodent plasmodia; (2) physiological and nutritional factors affecting susceptibility of vertebrate and vector hosts; (3) sterile immunity in malaria as exemplified by radical cure and by modification of challenge infection following exposure to non-living parasite products; (4) the role of the lymphoid-macrophage system in malaria; and (5) the excessive anaemia of malaria and its etiology.Gamma-globulin levels rise in malaria and remain high during latency. Protection is associated with IgG, which is passively transferable via the human placenta. Not all gamma-globulin is antibody, and not all antibody is protective. The fluorescent antibody technique and double diffusion in gel have been extensively used in exploring the kinetics of antibody production.New methods of harvesting plasmodia attempt to avoid protein degradation and to minimize contamination by host antigens. Plasmodia have proven to consist of a mosaic of antigens, and comparative studies by most of the accepted techniques have been started. Exoantigens have been described in fowl- and rodent-malarias. Relapse-variants of primate plasmodia have been shown to differ antigenically from their parent strains.


Differentiation of kidneys antigens in the human foetus.


Dose-response relationships of lymphocytic choriomeningitis viruses in mice and L cell tube cultures.


Abortive infection of Bacillus subtilis bacteriophage PBS1 in the presence of actinomycin D. Actinomycin D caused the irreversible loss of PBS1 phage infectious centers and PBS1-mediated transductants. The loss of infectious centers occurred only within the first 4 min after the addition of phage to cells. Actinomycin did not inactivate free phage or inhibit phage adsorption. Electron micrographs indicated that phage adsorbed to cells in the presence of actinomycin ejected their deoxyribonucleic acid (DNA) normally. However, when cells were infected in the presence of actinomycin, 15 to 22% of their (32)P-labeled DNA appeared in the medium, whereas only 1.5 to 7.2% of the (32)P-labeled DNA appeared in the medium during normal infection. Neither 8-azaguanine nor chloramphenicol caused a similar loss of PBS1 infectious centers or transductants. Actinomycin also caused the loss of SP10 infectious centers but it had no effect on SP01 or phi29 infections. We conclude that actinomycin causes abortion of PBS1 infection by inhibiting the uptake or retention of phage DNA into host cells. The immunity of SP01 and phi29 infections to actinomycin probably reflects differences in the penetration mechanisms of these phages.


Physical and genetic mapping of the SPO2 prophage on the chromosome of Bacillus subtilis 168. It has been found by density transfer and genetic mapping experiments that prophage SPO2 is linked to the antibiotic resistance marker ery-1 in Bacillus subtilis 168.


Progress in military medicine. United States Army.


The problem of drug abuse: alienation and the "alienist".


Genetic analysis of tox+ and tox- bacteriophages of Corynebacterium diphtheriae. A series of mutants derived from the temperate corynebacteriophages beta(tox+), gamma(tox-), and L(tox+) was isolated and characterized. In three-factor crosses between mutant beta phages the relative map order of the genetic markers determining extended host ranges (h and h') and loss of ability to lysogenize (c) was found to be h--c--h'. Recombination between markers was observed in matings between phage beta and the heteroimmune corynebacteriophages gamma and L. In such matings between heteroimmune phages the c markers of phages beta and gamma failed to segregate from the imm markers which determine the specificity of lysogenic immunity in these phages. The factor which directs the synthesis of diphtherial toxin during infection of appropriate corynebacterial hosts by toxinogenic corynebacteriophages is designated tox(+). It was possible to show that the tox(+) determinant of phage beta behaves as a single genetic element which occupies a position between the loci h and imm on the genetic map of this phage. Genetic recombination between mutants of phage beta occurred at very low frequencies in biparental matings performed by mixed infection of Corynebacterium diphtheriae C7(s)(-)(tox-). Considerably higher recombination frequencies were observed when lysogenic bacterial strains carrying one parental phage as prophage were induced by ultraviolet irradiation and then superinfected by the second parental phage. Maximal stimulation of genetic recombination between mutant beta phages was detected when superinfection followed ultraviolet irradiation of the lysogenic cells within a limited period of time. In matings between phages with incomplete genetic homology, the stimulation of recombination by ultraviolet radiation was much less effective.


Progressive patient care in an Army hospital.


The laser in medicine and biology: an appraisal.


Microbial flora of orthopaedic war wounds.


Neurosurgery in Vietnam 1967-1968.

Neurosurgery in Vietnam 1967-1968.


Longitudinal myelotomy in spastic paraplegia.


Case for diagnosis.

Case for diagnosis.


Staff reactions to the readmission of psychiatric patients.


Technical considerations in acute military vascular injuries of the extremities.


Fetal erythrocytes in maternal circulation of 155 Rh-negative women.


Significance of amniotic fluid analysis in Rh sensitization.


Laboratory diagnosis of diphtheria by the slide agglutination test.


Pathophysiology of endotoxin shock in primates and the effect of various therapeutic agents.


Ocular defects associated with homocystinuria.


Plasmodium malariae: transmission from monkey to man by mosquito bite. Anopheles freeborni mosquitoes were infected by feeding on New World monkeys, Aotus trivirgatus, infected with a Nigerian strain of Plasmodium malariae. The infection was passed to human volunteers through the bites of these mosquitoes, demonstrating the practicability of using a simian host for infection of mosquitoes with a third species of human malaria parasite and of the use of such mosquitoes to transmit the infection from monkey to man.


The polyphasic spike phenomenon.

The polyphasic spike phenomenon.


Preliminary survey of sensitivity of local strains of Pseudomonas pyocyanea to carbenicillin.


The study of simian viruses.

The study of simian viruses.


The medical problems of space travel. Third International Symposium on Basic Environmental Problems of Man in Space.


Planning and evaluation of health education services. Report of a WHO Expert Committee.


Community water supply. Report of a WHO Expert Committee.


Amoebiasis. Report of a WHO Expert Committee.


Early detection of cancer. Report of a WHO Expert Committee.


Mobilisation of iron by desferrioxamine in cases with low serum iron.


On the platelet levels in chronic iron deficiency anemia.


Thalassaemias and abnormal haemoglobins in SR macedonia. A survey of 2,861 children.


Recovery of haemopoiesis after cyclophosphamide.


Thrombotic thrombocytopenic purpura. Report of a case treated with splenectomy and steroids.


Refractory anemia with leukemic transformation and chromosomal change.


The occurrence oNeisseria gonorrhoeae in "routine" genital cultures.


A modified DOPA reaction for the diagnosis and investigation of pigment cells.


Metabolism of lactic acid in the Macacus rhesus monkey in profound shock.


On the nature of the pseudohybrids between denatured DNA and ribosomal RNA.


Effect of antilymphocyte serum on mortality of pseudomonas aeruginosa-infected rats.


The treatment of primary breast cancer. Comments.


Neurofibromatosis of the scalp.

Neurofibromatosis of the scalp.


A note on the blood groups of commonwealth immigrants to England.


Repeat prescriptions in hospital.

Repeat prescriptions in hospital.


Evaluation of shikimic acid as a precursor of pyocyanine.


A new resuspension medium for pyocyanine production.


Replication of Bacillus subtilis DNA during germination in 5 bromouracil containing medium and marker mapping.


Gene dislinkage in transfection of SP82G phage DNA.


Events occurring at the site of integration of a DNA molecule in Bacillus subtilis transformation.


A biochemical basis for apparent abortive transformation in Bacillus subtilis.


Blood groups (Rh, ABO) in the population of Gran Canaria (Canary Islands, Spain).


Spontaneous chromosomal exchanges in primates.


Control of encephalitis in laboratory monkeys by benzyl isothiocyanate.


Agglutination of platelets by a serum factor in the presence of EDTA. The platelets of a patient with reticulum-cell sarcoma were found to exhibit agglutination and cytopathic changes when her blood was treated with EDTA but not with other anticoagulants. Investigation showed that the phenomenon was caused by a serum factor that was probably an antibody.


Hematic and fluid barriers of the retina and vitreous body.


Observations on a new disinfectant.

Observations on a new disinfectant. A disinfectant formula (Resiguard) containing picloxydine digluconate, benzalkonium chloride, and a detergent was examined for antibacterial activity. Studies in vitro with simulated organic contamination were followed by realistic in-use tests employing items of anaesthetic and urological equipment heavily infected with Pseudomonas aeruginosa and contaminated with blood. These showed that a dilution of 1/80 was reliably bactericidal to this resistant organism. It is concluded that Resiguard is a valuable disinfectant which may reliably be employed in the cleaning and disinfection of items of delicate, non-autoclavable, hospital equipment.


Ascending projections of the substantia nigra in the rat.


Perkeo, the dwarf-jester of Heidelberg.

Perkeo, the dwarf-jester of Heidelberg.


Degenerative diseases of the central nervous system transmissible to experimental animals.


Toward a comprehensive food and nutrition program.


Benefits due to immunization against measles.


Radioisotopic studies of the glandular contribution of selected B vitamins in saliva.


Experiences of the public health service in training and using health auxiliaries.


A comprehensive care program for migrant farmworkers.


Health systems research to deliver comprehensive services to Indians.


Health defects and need for treatment of adolescents in low income families.


Salmonellosis in man in Poland, 1957-66.


Racial similarities and differences in family dental care patterns.


Better use of health professionals in New York City schools.


U.S. and Mexican medical students draft and pretest questionnaires for border health resources survey.


12-state survey of needs and interests in continuing education in public health.


Acutinhalatn toxicity of difluoro vapor in mice, rats, dogs, and monkeys.


Short-term inhalation exposure to bromotrifluoromethane.


Use of the rhesus monkey for predicting human response to eye irritants.


Cytoplasmic and ciliary connections between the inner and outer segments of mammalian visual receptors.


The topography of the afferent projections in the circumstriate visual cortex of the monkey studied by the Nauta method.


The effects of anticoagulants on platelet yields.


Erythrocyte metabolism and cellular deformability.


Abnormalities in synthesis of some subclasses of gamma-G in a family with two cases of ataxia telangiectasia.


Orbitography. A report on twenty consecutive cases.


When catarrhal ichors descend. The decline and fall of sophisticated Roman dentistry.


Now all out for fluoridation.

Now all out for fluoridation.


What to do when your lease expires.

What to do when your lease expires.


Mr. Porter keeps them blowing. A dentist at the service of old endentulous musicians.


Highly effective, completely safe. Report on the results of fluoridation after 11 years.


Dental aid after laryngectomy.

Dental aid after laryngectomy.


Putting your clearly in the picture.

Putting your clearly in the picture.


The value of blood culture in the diagnosis of cerebrospinal meningitis with special reference to the isolation of meningococci.


Experimental variola in monkeys. I. Studies on disease enhancing property of cortisone in smallpox. A preliminary report.


The oral absorption of doxycycline by man.


Plasma histaminase activity in cases of tropical eosinophilia.


Polysomes from Bacillus subtilis and Bacillus thuringiensis.


Some observations on the bleaching of ethylenediaminetetraacetic acid on green barley leaves. Green barley leaves (Hordeum vulgaris) floated on the surface of 0.05 m ethylenediamine disodium tetraacetate, EDTA-2Na, pH 7.0 and exposed to light (5000 lux) at 25 degrees exhibited a marked bleaching (EDTA-bleaching) visible to the naked eye and paralleled by a striking reduction in content of chlorophylls a and b. This loss of color did not occur in controls which were treated with H(2)O instead of EDTA (water controls). In darkness the leaves in the water controls were bleached while EDTA-treated leaves retained all their color.EDTA bleaching was observed only in intact leaves. When leaves were boiled EDTA protected their pigment against photodecomposition. Without EDTA boiled leaves were bleached completely in light. When intact green leaves which had been floated on water and exposed to light for 48 hr were treated with boiling ethanol or acetone, the chlorophylls extracted by this procedure did not undergo bleaching if EDTA were present in solution. Under these conditions a green fine grain precipitate formed which was insoluble in ethanol or acetone and was stable in light or darkness.EDTA bleaching of green barley leaves was inhibited by KCN, and by the addition of casein hydrolysate.


Ocular anomalies of incomplete albino cattle: ophthalmoscopic examination.


The effects of nitroglycerin on myocardial blood flow in man, measured by coincidence counting and bolus injections of 84-rubidium.


General anesthesia in infrahuman primates.


Approaches to moderating the increases in medical care costs.


Experimental observations on flufenamic, mefanamic and meclofenamic acids. II. Toxicology in animals.


Studies on the chymotrypsin C and papain fragments of human immunoglobulin M.


Experimental observations on flufenamic, mefenamic and meclofenamic acids. 3. Metabolic disposition.


Post-payment medical expense coverage: a proposed salvation for insured and insurer.


Subtilisin cleavage of bovine plasma albumin. Reversible association of the two primary fragments and their relation to the structure of the parent protein.


Ribulose 1,5-diphosphate carboxylase from Hydrogenomonas eutropha and Hydrogenomonas facilis. I. Purification, metallic ion requirements, inhibition, and kinetic constants.


Ribulose 1,5-diphosphate carboxylase from Hydrogenomonas eutropha and Hydrogenomonas facilis. II. Molecular weight, subunits, composition, and sulfhydryl groups.


Isolation and characterization of a phytohemagglutinin from the lentil.


Excision of the developing mandibular third molar by lateral trepanation. A new approach with a reappraisal of the enucleation of the unmineralised germ.


Protein synthesis in isolated nuclei and nucleoli of HeLa cells.


A comparison of electron density and hepatocarcinogenic activity for various derivatives of 4-dimethylaminoazobenzene.


Transient mental deficits associated with recurrent prolonged epileptic clouded state.


EEG studies of relatives of probands with focal epilepsy who have been treated surgically.


A case of petit mal status with myoclonus.


A study of automatisms associated with the absence of patit mal.


Generalized kinetics of reversible inhibition and activation.


The effects of histamine on the myocardial circulation.


Memory deficit in monkeys brain damaged by asphyxia neonatorum.


Effects of ablations of temporal cortex upon speech sound discrimination in the monkey.


Localization of ceruloplasmin biosynthesis in human and monkey liver cells and its copper regulation.


Chemical constituents of the antibiotic fraction of Arnebia nobilis.


Identification of ceruloplasmin in human liver cells by fluorescent antibodies and absence of this protein in Wilson disease.


The preparation and antibacterial activity of 3,3'-bis(trifluoromethyl) tetranitrodiphenylamine.


The RBE of 14 MeV neutrons. Observations on colony-forming units in mouse bone-marrow.


Deoxyribonucleic acid base composition of the genus Lactobacillus. Deoxyribonucleic acids of 45 strains of Lactobacillus and 5 strains of Bifidobacterium which had been analyzed for base composition by chromatographic means were examined at equilibrium in a CsCl density gradient. Regression analysis showed that there have been systematic errors involved in the estimation of guanine plus cytosine (GC) content by the chemical method, and that the relation between buoyant density and base composition is indeed linear and best fitted by the equation GC = 10.309 (rho-1.662), which compares well in slope with the equation of Schildkraut, Marmur, and Doty. With the improved data obtained in this study, the specific groupings of the species of both genera were reevaluated.


Purification of staphylocidal beta-lysin from rabbit serum. A cationic protein of rabbit serum bactericidal for Staphylococcus aureus was purified. The specific activity per unit of protein of the purified staphylocidal preparation was approximately 37,000 times greater than that of the serum from which it was isolated. Similar techniques were used to purify serum beta-lysin active against Bacillus subtilis approximately 24,000 times. The staphylocidal activity cannot be attributed to the same beta-lysin active against B. subtilis, lysozyme, or antibody-complement systems. The concentrations of staphylocidal beta-lysin in the sera of the five mammalian species studied did not correlate with their beta-lysin activities against B. subtilis. The two beta-lysins are similar in that both were heat-stable, sensitive to trypsin digestion, had molecular weights near 6,000, and were found in higher concentrations in serum than in plasma. Furthermore, similar techniques can be used to absorb and elute both substances in highly purified forms using cellulose asbestos filter pads and ion exchange chromatography on carboxymethyl cellulose. In contrast to the beta-lysin against B. subtilis, the staphylocidal beta-lysin was not released from blood platelets, and it was inactive in the presence of heparin, sodium citrate, sodium oxalate, ethylenediaminetetraacetic acid, acidic phospholipids, and acid pH values. A variety of proteins, including those of normal serum, preferentially inhibited the bactericidal activity of staphylocidal beta-lysin but not the beta-lysin against B. subtilis.


Neisseria gonorrhoeae. II. Colonial variation and pathogenicity during 35 months in vitro. During 35 months of selective in vitro cultivation, Neisseria gonorrhoeae cells retained their virulence for humans and were shown to be closely related to a particular colonial morphology. Saline-autoagglutinability was the only other characteristic distinguishing virulent from avirulent cells. Human responses to challenge with cells of the different colonial types were studied for their relationships to virulence or avirulence.


Degradation of uric acid by certain aerobic bacteria. We have isolated and identified nine cultures of aerobic bacteria capable of growing on an elective medium containing uric acid as the only source of carbon, nitrogen, and energy. Four of these cultures were identified as Aerobacter aerogenes, two as Klebsiella pneumoniae, and the remainder as Serratia killiensis, Pseudomonas aeruginosa, and Bacillus species. Another culture identified as P. fluorescens required both glucose and uric acid for growth. When 23 laboratory stock cultures were inoculated into the uric acid medium, A. aerogenes, B. subtilis, Mycobacterium phlei, P. aeruginosa, and S. marcescens were able to grow. These five cultures also grew when the uric acid was replaced with adenine, guanine, hypoxanthine, xanthine, or allantoin, but growth was poor. In all of these media, including the uric acid medium, addition of glucose along with the nitrogenous compounds yielded good growth. Induction experiments demonstrated that the ability of A. aerogenes, K. pneumoniae, P. aeruginosa, P. fluorescens, S. kiliensis, S. marcescens, B. subtilis, and Bacillus sp. to degrade uric acid is an induced property. Of these organisms, only Bacillus sp. accumulated a small amount of intracellular uric acid.


Paraffin oxidation in Pseudomonas aeruginosa. I. Induction of paraffin oxidation. The induction of paraffin oxidation in intact cells of Pseudomonas aeruginosa was investigated. Oxidation of (14)C-heptane by cell-free extracts of adapted cells showed that the activity of whole cells is a reliable reflection of the synthesis of the first enzyme in the degradation of n-alkanes. Induction was significantly affected by glucose and could be completely repressed by malate. The amino acids l-proline, l-alanine, l-arginine, and l-tyrosine exhibited a rather low repressor action. Malonate, a nonrepressive carbon source, allowed gratuitous enzyme synthesis. A number of compounds which did not sustain growth were found to be suitable substitutes for paraffins as an inducer. Among these were cyclopropane and diethoxymethane. The induction studied under conditions of gratuity with the latter compound as an inducer showed immediate linear kinetics only at saturating inducer concentrations. With n-hexane as the inducer, a lag time was always observed, even when high concentrations were used.


Immunological homology between crystal and spore protein of Bacillus thuringiensis. Spore suspensions containing about 0.3% crystals and crystal suspensions containing about 0.1% spores were obtained from cultures of Bacillus thuringiensis by extraction with a two-phase system. Both preparations were tested for the presence of contaminating material from vegetative cells and were judged to be clean. Solutions of spore protein were obtained by extracting broken spores with phosphate buffer followed by extraction with either alkali- or urea-mercaptoethanol. The alkali spore or urea spore extracts had the same isoelectric point as crystal protein solubilized with these reagents. An antiserum prepared against alkali crystal solution precipitated alkali or urea spore extracts and crystal solutions but not phosphate spore extracts or extracts of whole cells. Lines of identity between spore and crystal precipitates were observed by using the Ouchterlony double-diffusion technique. Absorption of the antiserum with an excess of urea spore extract caused a disappearance of the precipitin bands originating from the spore protein and the homologous bands from the crystal protein. The results suggest that the crystal and endospore contain one or more common proteins.


Secretion of alkaline phosphatase subunits by spheroplasts of Escherichia coli. Under conditions that permitted continued protein synthesis, spheroplasts of Escherichia coli were unable to form active alkaline phosphatase, although they synthesized protein that was antigenically related to alkaline phosphatase subunits. This cross-reacting protein was primarily detected in the medium of the spheroplast culture, and it had properties that closely resembled those of the alkaline phosphatase subunit. These results suggest that formation of the active alkaline phosphatase dimer by intact E. coli cells proceeds by a pathway in which inactive subunits released from polyribosomes diffuse through the bacterial cell membrane to a periplasmic space where subsequent dimerization to active enzyme occurs. This pathway provides a possible mechanism for the specific localization of this enzyme to the E. coli periplasmic space.


Intramolecular heterogeneity of the deoxyribonucleic acid of temperate bacteriophages. Spectral changes accompanying the thermal denaturation of phage deoxyribonucleic acid suggested that lambda is not unique in possessing large-scale intramolecular heterogeneity and nucleotide clustering; instead, lambda seems to share this property with other enteric phages.


Inhibition of fibrinogen reaction by polysaccharide of encapsulated Staphylococcus aureus. Encapsulated and nonencapsulated strains of Staphylococcus aureus which lack coagulase or clumping factor (bound coagulase), or both, were examined for the antigen associated with the fibrinogen-cell clumping reaction. Extracts of the cells were tested for the ability to react with fibrinogen or to inhibit fibrinogen precipitation. Antisera prepared against encapsulated (coagulase-positive, clumping factor-negative) variants, as well as against nonencapsulated wild-type (coagulase-positive, clumping factor-positive) S. aureus strains, contained high titers of clumping-inhibiting antibody. When coagulase-negative, clumping factor-negative mutants were the immunizing agents, antisera contained no demonstrable clumping-inhibiting antibody. Phenol extracts of all coagulase-positive strains tested precipitated fibrinogen, regardless of the ability of cells to clump in the presence of fibrinogen. Polysaccharide extracts of encapsulated, clumping factor-negative strains inhibited this fibrinogen-precipitating activity, whereas similar extracts of nonencapsulated staphylococci did not inhibit the fibrinogen reaction. From these results, it appeared that the coagulase-positive, encapsulated staphylococci which do not clump in fibrinogen solution possess clumping factor, but that their capsular polysaccharide inhibits clumping activity. These findings suggested a closer association of clumping factor and coagulase than is now recognized.


Comparison of staphylococcal clumping factor and protein A.


An outline of the problems of immunogenetics and reproduction.


Lactic acid accumulation in connection with fructose infusion.


The development of cellular hypersensitivity in man after a primary immunization.


Plate assay methods for amino acids. 5. Usefulness of nine strains of lactic-acid bacteria for the assay of methionine.


Plate assay methods for amino acids. 6. Comparative assays for methionine carried out by the plate method, the tube method and the ion-exchange column chromatographic method.


Studies of catecholamine shock. I. Disseminated intravascular coagulation.


The Zenker's (hypopharyngeal) diverticulum.


Studies of catecholamine shock. II. An experimental model of microangiopathic hemolysis.


Combined external and endoscopic hypopharyngeal diverticulectomy.


Implantation of electrodes near individual vestibular nerve branches in mammals.


Proteins of human perilymph. II. Identification of additional components by immunodiffusion.


Cochlear pathology following stapes stimulation.


Studies on the Xh antigen in human serum.


In vivo study of IgM rheumatoid factors from mixed cryoglobulins.


Rejection of skin grafts from mice chronically infected with Lymphocytic choriomeningitis virus by non-infected syngeneic recipients.


Pyelonephritis. X. Microbial interference in streptococcal infections in the rat kidney.


Frequency of G-6-PD deficiency among some Iranian ethnic groups.


Comparaive studies of American and African trypanosomiasis.


Health factors involved in working under conditions of heat stress. Report of a WHO Scientific Group.


Genetic counselling. Third report of the WHO Expert Committee on Human Genetics.


Non-experimental aural pathology in rhesus monkeys (Macaca mulatta).


Tympanic membrane. II. Pars flaccida.

Tympanic membrane. II. Pars flaccida.


Electron microscopic study of semicircular canal organs and otolith organs of squirrel monkeys after administration of streptomycin sulfate.


Neutral proteases of the genus Bacillus.


Pituitary regulation by the hypothalamus: functions of the median eminence gland.


Nucleoprotein complexes: possible subunits of chromosomes.


The prevalence of sulfadiazine resistant meningococci in Oklahoma, 1968.


Potentiation of cell growth inhibition by furanosides.


Effect of endocrines on central nervous system. II.


Ultraviolet light alteration of cellular deoxyribonucleic acid in vivo. DNA after irradiation with ultraviolet light was immunogenic in rabbits and elicited serum antibodies reacting specifically with UV-irradiated DNA. The serological reactions were demonstrated by immunodiffusion, complement fixation, and immunofluorescence. By immunofluorescence, antisera reacted with cell nuclei of irradiated tissue sections but not with unirradiated tissue. This method was employed to show the presence of UV lesions in tissues of mice exposed to UV light. UV lesions in DNA were present in nuclei of epidermal cells, and in heavily irradiated animals they were also detected in the corium immediately below the epidermis. The method is useful not only for directly demonstrating UV lesions of DNA but also for localizing such lesions in tissues.


Arthrography of the ankle: its use in the evaluation of ankle sprains.


Nonmalignant and malignant changes in hamsters inoculated with extracts of human osteosarcomas.


WHO Expert Committee on Medical Rehabilitation. Second report.


The vascular supply of the face in long-snouted primates (Papio and Mandrillus).


The fine structure of the parotid gland of the spider monkey (Ateles paniscus).


On histochemical and histoenzymological variations in tissue mast cells.


Histochemical analysis of the structural stability of biopolymers (preliminary data).


Dehydrogenase patterns of the experimental epileptogenic cortical focus in the rat. A correlation of histochemical and electrophysiological findings.


Histochemical study of the implication of certain nervous tissue hydrolases in effort and exhaustion.


Histochemical demonstration of heavy metals with the sulfide-silver method. A methodological study.


Acylglucoses in corynebacteria.

Acylglucoses in corynebacteria.


Chemical derivatives of subtilisins with modified proteolytic activities. I. Nitration and iodination of subtilisin type Carlsberg and subtilisin type Novo.


The degradation of the B-chain of oxidized insulin by two subtilisins and their succinylated and N-carbamylated derivatives.


Comparative pathology after intrathecal endotoxin in the rabbit, dog and monkey.


Connection between a mitochondrion and endoplasmic reticulum in liver.


Treatment of burns: experiences with polyethylene glycol dressing.


A case history of haemolytic disease of the newborn due to anti-Duffy (anti-Fya).


Sustaining life during fulminant hepatic failure.


Drug control and the British Health Service.


The importance of changes in Diagnostic Standards and Classification of Tuberculosis.


Role of pituitary gland in melanin synthesis in tadpole skin.


On the mode of action of a new antifungal antibiotic, pyrrolnitrin.


Glyoxylate carboligase of Escherichia coli. Identification of carbon dioxide as the primary reaction product.


Isolation and properties of porcine thyrotropin-releasing hormone.


Polyacrylamide gel electrophoresis of rhesus monkey (Macaca mulatta) pituitary gland extracts.


Cyanide metabolism by Bacillus megaterium.


Artificial feeding of infant rats by continuous gastric infusion.


What arthritis costs you.

What arthritis costs you.


Increase in plasma folate levels of rabbits after phenylhydrazine-hemolysis.


The use of sulphydryl containing drugs and chelating agents to reduce renal irradiation during 203Hg chlormerodrin scintigraphy.


The production of anti-D preparations used in the prevention of Rh immunization.


Suppression of primary RH immunization by passively-administered antibody. Experiments in volunteers.


Interaction of ethylenediaminetetraacetic acid with plasma proteins. I. The binding of 14C-EDTA to plasma proteins.


Postdiaphragmatic disposition of the pars sympathica and major autonomic ganglia of the goat (Capra hircus).


Identification and differentiation of human milk.


A study of the calcium-phosphorus ratio of different milks.


Estimation of toxic doses of strychnine hydrochloride in rats and dogs.


The hypothalamic regulation of thyrotrophic hormone secretion during the estrous cycle.


Experimental investigations on the regulation of the blood circulation of the spinal cord. II. The influence of vaso-active substances on the haemodynamics of the spinal cord under physiological conditions.


The stability of phenylephrine.

The stability of phenylephrine.


The Lennox-Gastaut syndrome: a severe type of childhood epilepsy.


Genetic models of epilepsy with special reference to the syndrome of the Papio papio.


The hyperexcitable neuron as a model for the laboratory analysis of anticonvulsant drugs.


The cortical representation of the retina in squirrel and rhesus monkeys and its relation to visual acuity.


Extrageniculostriate vision in the monkey: discrimination of luminous flux-equated figures.


Age-related alterations in the structure of nucleoprotein. IV. Changes in the compoistion of whole histone from rat liver.


Phagocytosis of bacteria by eosinophils in infectious-related asthma.


The immunogenicity of pyridine-extracted, alum-precipitated ragweed.


Bacterial endocarditis: current concepts.


"Transcriptional mapping". I. Introduction to the method and the use of actinomycin D as a transcriptional mapping agent. The transcription process can in theory be used for genetic mapping on the "intraoperonal" level. We have shown that such an approach, utilizing actinomycin D to create blocks to the travel of the transcription enzyme, is indeed feasible, and the effects of actinomycin D on the synthesis of general pulse-labeled RNA are reported herein.


Two rapidly labeled RNA species in the polysomes of antibody-producing lymphoid tissue. Popliteal lymph nodes of rabbits stimulated to produce antibodies were pulse labeled in vivo by direct injection of (3)H-uridine. RNA was then extracted from isolated polysomes and single ribosomes. Fractionation of this RNA by polyacrylamide gel electrophoresis revealed the synthesis of two discrete peaks of labeled RNA migrating in the region between 18S ribosomal and 4S transfer RNA. These peaks were found in the RNA extracted from polysomes but were absent from single ribosomes. When the polysomes were disrupted by EDTA treatment the RNA species no longer appeared as rapidly sedimenting material. These two RNA's have molecular weights near 2.2 x 10(5) and 3.7 x 10(5) daltons. The chemical and biological properties of these species as well as the molecular weights are consistent with those expected for monocistronic messenger RNA coding for the antibody L and H chains, respectively.


Spectral and dosimetric characteristics of soft X rays for studies in radiobiology.


Absent constrictive response of wound vessels to intra-arterial vasopressors: angiographic observations.


Mutants with altered DNA-base composition.


Rickettsial diseases.

Rickettsial diseases.


Parasitic cutaneous lesions in a Cod (Gadus morhua L.).


Large-animal ophthalmology.

Large-animal ophthalmology.


Immunochemical studies on the heterogeneity of human growth hormone (HGH). Electrophoretical pattern and behaviour in gel filtration of HGH in various pituitary extracts.


Synovectomy, a new approach to haemophilic arthropathy.


Prophylaxis of joint hemorrhages in hemophilia.


Different blast kinetics in acute myeloblastic and lymphoblastic leukaemia. A hypothesis of different stem cell origni.


Beta-thalassaemia minor with an unusually high prevalence among siblings.


An in vitro study of the effect of arsenic (As2O3) on blood clotting.


A case of the Rubinstein-Taybi syndrome.


Doubling of the papilla.

Doubling of the papilla.


Studies on the reduction of a human 19S immunoglobulin M. 1. Reduction of a 19s immunoglobulin M with 3mm-mercaptoethanol or 0.05-0.5mm-dithiothreitol followed by alkylation gave sedimentation patterns indicating products compatible with structures consisting of one, two, three, four and five 7s sub-units. This supports the concept of a five-sub-unit structure for immunoglobulin M. 2. Reduction with 0.125mm-dithiothreitol or 20mm-cysteine produced 7s sub-units that could not be dissociated into chains in m-propionic acid. 3. By labelling (with iodo[2-(14)C]acetic acid) the thiol groups liberated during reduction with 0.125mm-dithiothreitol, it was possible to identify the tryptic peptides involved in the disulphide bridges that link the 7s sub-units together (inter-sub-unit bridges). 4. By further reducing and labelling (with iodo[2-(14)C]acetic acid) the 7s sub-units produced by 0.125mm-dithiothreitol, it was possible to identify tryptic peptides derived from intra-sub-unit bridges. 5. Sub-units produced by reduction with 20mm-cysteine proved to be unsuitable for distinguishing between inter-sub-unit bridges and intra-sub-unit bridges. 6. The possible arrangement of the interchain disulphide bridges was deduced.


Studies on tryptophan synthetases from various strains of Bacillus subtilis. 1. Tryptophan synthetase B of three strains of Bacillus subtilis was prepared from ;exo-protoplastic' and ;endo-protoplastic' fractions; the enzyme from ;exo-protoplastic' fraction was purified 30- to 120-fold by ammonium sulphate precipitation and DEAE-cellulose column chromatography; the latter step separated this enzyme from tryptophan synthetase A, tryptophanase and proteolytic enzymes, but the purified preparations were not stable. 2. The activity of tryptophan synthetase B did not depend on the presence of tryptophan synthetase A. 3. Tryptophan synthetases B of the strains tested differed in their utilization of 2- and 7-methylindole as compared with indole; this suggests that these tryptophan synthetases B are not identical.


Management of rheumatoid arthritis.

Management of rheumatoid arthritis.


Stepwise cleavage of rabbit immunoglobulin G by papain and isolation of four types of biologically active Fc fragments. Four types of Fc fragments of different sizes were isolated by papain treatment of rabbit immunoglobulin G under various conditions and by subsequent chromatographic procedures. 1. Brief digestion at neutral pH without reduction produced a molecule in which the Fab and Fc fragments were still linked by a pair of labile disulphide bridges, and the Fc fragment released by cleaving these bonds, called 1Fc fragment, contained a portion of the ;hinge' region including an interchain disulphide bridge. Both complement-binding and guinea-pig skin-binding activities were retained by this fragment, which had mol. wt. 48000. 2. Prolonged digestion at neutral pH of immunoglobulin G whose labile inter-heavy-chain disulphide bridges had been reduced removed the ;hinge' region, giving mFc fragments (mol. wt. 46000), which lacked the capacity to bind guinea-pig skin but retained the antigenic as well as the complement-binding activities of 1Fc fragment completely. 3. Digestion at pH5.0 yielded a smaller fragment, sFc (mol. wt. 40000), which was no longer able to bind complement. Though the antigenic structure was intact, sFc fragment was curiously unable to precipitate with antibodies to the N-terminal determinants. 4. Fragment stFc (mol. wt. 25000), representing the C-terminal portion of Fc fragment, was formed from all the larger fragments by digestion at pH4.5. Only the C-terminal antigenic determinants were retained by stFc fragment.


The effect of millipore filtration on four spectrophotometric methods of bilirubin determination in amniotic fluid.


Effects of atropine on aqueous humor dynamics in the vervet monkey (Cercopithecus ethiops).


An early twin blastocyst of the golden lion marmoset, Leontocebus rosalia L.


Properties of the crystallins of the chick in terms of their subunit composition.


The chromosomes of Saguinus fuscicollis illigrei (Pucheran, 1845) and Aotus trivirgatus (Humboldt, 1811).


Introductory techniques in the formation of pigtail monkey troops.


On the daily behaviour and spacing of howling monkey groups.


The Nilgiri langur (Presbytis johnii) troop: its composition, structure, function and change.


Nutrition in the aged: review of the literature.


Preservation of the ultrastructure of Bacillus subtilis by chemical fixation as verified by freeze-etching. The present study on the ultrastructure of Bacillus subtilis was undertaken in order to examine by means of the freeze-etching technique possible structural changes occurring during the chemical fixation procedure (Ryter-Kellenberger (R-K) fixation). Three stages were followed by freeze-etching, viz.: (a) fixation in osmium tetroxide, (b) fixation in osmium tetroxide and posttreatment with uranyl acetate, and (c) fixation in osmium tetroxide, posttreatment in uranyl acetate, and dehydration in a graded series of acetone. Preparations were made after each stage in the presence of 20% glycerol. Good preservation of ultrastructure was observed, after any of the three treatments, of the outer surface of the plasma membrane, and the inner surface of the plasma membrane. No alteration in fracturing properties could be observed. However, if we are to judge by the results of freeze-etching, any of the successive steps of the chemical fixation procedure achieve strong contrast between the nucleoplasmic region and the cytoplasm. Dependent on the quality of fixation, very delicately preserved DNA fibrils or strongly aggregated ones were seen. It appears that R-K fixation is capable of producing more or less distinctly visible changes in the native state of the nucleoplasm in young cells of B. subtilis.


Intestinal capillaries. II. Structural effects ofEDTA and histamine. Perfusion of the fenestrated capillaries of the intestinal mucosa of the rat with 0.05-0.1 M EDTA removes the diaphragms of the endothelial cells and detaches these cells from one another and from the basement membrane. The latter, even when completely denuded, retains effectively particles of 340 A (average) diameter. Perfusion with histamine (1 microg/ml) results in partial removal of fenestral diaphragms, occasional detachment of the endothelium from the basement membrane, and focal separation of endothelial intercellular junctions.


The cortico-thalamic projections from the sensorimotor cortex of Galago crassicaudatus.


An association of Rh type and gynaecological disease with twinning.


Subcellular localization of rodent and of monkey intrinsic factor.


Alteration of superficial bacterial flora in severely ill patients: I.


Low temperature dry heat sterilization.

Low temperature dry heat sterilization.


Physical and chemical deterioration of endodontic reamers during mechanical preparation.


Age distribution of antibodies to EB virus in Swedish females as studied by indirect immunofluorescence on Burkitt cells.


Protein A from Staphylococcus aureus. 8. Production of protein A by bacterial and L-forms of S. aureus.


Increased stability of orthodontically rotated teeth following gingivectomy in Macaca nemestrina.


Cataract produced by tyrosinase and tyrosine systems in rabbitens in vitro. 1. Cataracts were developed by incubating rabbit lenses for 22hr. at 37 degrees in a culture medium containing tyrosine and tyrosinase (EC 1.10.3.1). 2. A 45% diminution in the content of GSH and significant inhibition of glucose 6-phosphate dehydrogenase (EC 1.1.1.49) activity were observed in the cataractous lenses compared with controls. 3. GSSG accumulated in both cataractous and control lenses. Significant amounts of GSSG were transported outward from the cataractous lenses and small amounts from control lenses. 4. Transport of GSSG from rabbit lens incubated in a diffusate of plasma from a naphthalene-fed rabbit was also observed. 5. GSSG was found in the aqueous humour obtained between 2 and 24hr. after feeding of naphthalene to rabbits; subsequently the GSSG in the aqueous humour decreased to almost undetectable amounts in 48hr.; in controls, GSSG was not detectable. 6. A possible mechanism of formation of experimental and senile cataract is briefly discussed.


Immune reactions in polysaccharide media. The effect of hyaluronate, chondroitin sulphate and chondroitin sulphate-protein complex on the precipitin reaction. The influence of the connective-tissue polysaccharides hyaluronate, chondroitin 4-sulphate and a chondroitin 4-sulphate-protein complex (PP-L) from cartilage on the precipitin reaction was investigated. In a system consisting of (125)I-labelled human serum albumin and the immunoglobulin G fraction from rabbit anti-albumin sera, the precipitation is greatly increased in the region of antigen excess. This effect depends on the concentration, molecular weight and configuration of the polysaccharide. The increase parallels a decrease in the amount of soluble immune complexes in the supernatant. It is suggested that the effect is due to steric exclusion of the complexes from the domains of the polysaccharides. The possibility that such a mechanism might enhance precipitation of antigen-antibody complexes in certain pathological conditions is discussed.


The mechanism of shock in acute gastric dilatation: an angiographic study in monkeys.


Measurement of plasma volu using 99Tcm, and 113Inm labelled proteins.


The chick embryo neutralization test in the assay of meningococcal antibody. 1. Infection of the embryo with Neisseria meningitidis. Present methods of controlling meningococcal cerebrospinal meningitis have failed to contain the disease. This has led to the search for effective vaccines and to the development of methods for assaying the potency of these vaccines, as well as for measuring the immune response of the individual. The feasibility of using the sero-protection test in embryonated eggs for the assay of meningococcal antibody has been investigated. The paper describes detailed investigations of the infectious process established by injecting Neisseria meningitidis by various challenge routes. Embryos, even 10-12 days old, showed high susceptibility to meningococci injected via the yolksac or chorioallantoic vein, and lesser susceptibility when injected via the intra-allantoic and chorioallantoic routes. The deaths of intravenously inoculated embryos coincided with the multiplication of the organisms and ensuing septicaemia. A study of the infection in embryos which had died, or were killed, 18-24 hours after inoculation showed a specific localization of the organisms in the brain.