A possible role for prostaglandin E1 as a modulator for temperature regulation in the central nervous system of the cat.


A structural approach to inservice education.


Among regional medical programs--an enduring commitment.


Significant others.

Significant others.


The time course of ischaemic neuronal changes in the primate brain following profound arterial hypotension, air embolism and hypoglycaemia.


A counseling program for nurses.

A counseling program for nurses.


A self study tool for independent learning in nursing.


A private university contributes to community health.


Inservice education in a clinical research hospital.


Milwaukee's N. I. S. E. organization.

Milwaukee's N. I. S. E. organization.


The development of leadership in nursing.


Nursing administration in long-term care facilities: a dual-kardex system.


Developmental nursing.

Developmental nursing.


Four steps to self evaluation or what to do until the accreditors arrive.


Continuing education in nursing comes to Mississippi.


The American Nurses' Association and continuing education.


Therapeutic abortion as a possible source of Rh immunization.


Learning in rhesus monkeys after varying amounts of prefrontal lobe destruction during infancy and adolescence.


Social behavior deficits of free-ranging monkeys after anterior temporal cortex removal: a preliminary report.


Effect of low-level, low-frequency electric fields on EEG and behavior in Macaca nemestrina.


Cortical areas exerting presynaptic inhibitory action on the spinal cord in cat and monkey.


[Isoenzymes of lactate dehydrogenase during the 1st stages of development of the ovum of trout (Salmo irideus, Gibb)].


Round table.

Round table.


Meeting on disodium cromoglycate (Intal-Lomudal).


Functional morphology of the growing lung.


Inhibition of the bronchial reaction induced by allergen inhalation in children.


Treatment of asthma with DSCG in juveniles during and after hospitalization.


Comparisons of serum lipid and uric acid content in white and Negro men.


Clinical value and mode of action of chlorpropamide in diabetes insipidus.


Further studies on a fetal pigment.

Further studies on a fetal pigment.


Lesions of the pontine tegmentum and conjugate gaze paralysis.


Cerebrospinal fluid immunoglobulin G in childhood. Measurement by electroimmunodiffusion.


Corneal ulcer due to Pseudomonas aeruginosa. A comparison of the disease in California and El Salvador.


Irradiation of early carcinoma of the larynx. Significance of tumor extent.


Long-term results with cryosurgery for Meniere's disease.


Cerebral circulation. Effects of vasodilating drugs on blood flow and the microvasculature of ischemic and nonischemic cerebral cortex.


Middle ear insufflation.

Middle ear insufflation.


Molecular species of lecithins from various animal tissues.


Unwanted effects of anticonvulsant drugs.


A double-blind comparison of nitrazepam versus diazepam in myoclonic seizure disorders.


Potential changes associated with rapid eye movement in the calcarine cortex.


Auditory thresholds in monkeys asphyxiated at birth.


Indigogenic methods for glycosidases. 3. An improved method with 4-Cl-5-Br-3-indolyl-beta-D-fucoside and its application in studies of enzymes in the intestine, kidney and other tissues.


Lithium carbonate (Eskalith ). For control of manic episodes in manic-depressive psychosis.


Inhibition of nucleolar RNP synthesis by cycloheximide as studied by high resolution radioautography.


Actinomycin D; renewed RNA synthesis after removal from mammalian cells. Suppressed RNA synthesis exceeded control rates 2 hours after removal of actinomycin from serial lines of green monkey kidney cells in exponentially dividing monolayer cultures. Enhanced synthesis was directly related to the dose of actinomycin, and release from inhibition was temperature dependent. A broad range of RNA sizes was made after removal of drug, but cytoplasmic and nucleolar sedimentation profiles were abnormal for at least 20 hours.


Histidinemia: detection by routine newborn screening and biochemical observations on three unrelated cases.


Prophylactic use of antimicrobial agents.


Studies of the nerve growth factor by micro-complement fixations. Effects of physical, chemical and enzymatic treatments.


Glycoprotein I of Phaseolus vulgaris. Homogeneity and enzymic properties.


Studies on fraction I protein. II. Comparison of physical, chemical, immunological and enzymatic properties between spinach and tobacco fraction I proteins.


Recovery of performance following electroanesthesia in primates.


Development of the oral flora in new born monkeys (Macaca irus).


Bacillus megaterium neutral protease, a zinc-containing metalloenzyme.


Glucose and free fatty acid metabolism in perfused hearts of normal and hypothyroid monkeys (Saimiri sciureus).


Cleavage of human ova in vitro.

Cleavage of human ova in vitro.


Study of creatine kinase in the invertebrate.


Studies of DNA-induced heritable alteration of mammalian cells. An intercellular interaction between mouse Ehrlich ascites tumor and non-malignant Chinese hamster cells occurred when these were co-cultured. That the intercellular processes which formed had emanated from the EA cells was revealed by immunofluoroscopy using anti-EA antiserum, and by direct microscopic examination. A passage of DNA from the EA to the CH cells was also observed. On long-term co-culture, new cell forms arose which were isolated, cloned, and propagated. They showed a CH karyotype and had acquired oncogenic potential and the ability to synthesize murine-specific antigens. These same heritable properties were also acquired by CH cells following their exposure to DNA isolated from EA cells.


Preliminary observations on the effect of halothane and oxygen anesthesia on the immunologic response in man.


Experimental formation of gallstones in the squirrel monkey.


The fate of challenge schistosome cercariae in a monkey immunized by cercariae exposed to high doses of x-irradiation.


In vitro sensitivity of measles virus to 6-azauridine.


Deoxyribonucleic acid (DNA) and antibodies to DNA in the serum of hamsters and man infected with schistosomes.


Hematocrit value, blood volume, and cardiac output in extensive burns.


An assessment of immunosuppressive agents in experimental peripheral nerve transplantation.


Supranormal dietary intake in thermally injured hypermetabolic patients.


Report on a hand-reared squirrel monkey (Saimiri sciureus).


Rubella and the eye specialist.

Rubella and the eye specialist.


Sex differences in emotional behaviour in mammals including man: endocrine bases.


Determination of methanol and its application to measurement of pectin ester content and pectin methyl esterase activity.


Audition.

Audition.


Spatial vision.

Spatial vision.


Growth retardation and thyroxine-binding globulin deficiency.


'Little leopard' syndrome. Description of 3 cases and review of 24.


Isolation of a macroglycopeptide from human platelets.


Pigments in the red nucleus and substantia nigra in man and in representative old and new world monkeys.


Bacterial infection in the fetus and newborn.


The synchronization of Bacillus subtilis by the relaxation of a division control system.


Naphthylamidases of Sarcina lutea.

Naphthylamidases of Sarcina lutea.


Comparison between the attigenic components extracted from virulent and avirulent strains of mycobacteria.


The regulation of phenolic acid sysdtness in Bacillus subtilis.


The anatomy of "paleocortex". A critical review.


Social signals in squirrel monkeys: analysis by cerebral radio stimulation.


Studies of colonic carcinoma antigens.

Studies of colonic carcinoma antigens.


Formation of protoplasts from resting spores. Coat-stripped spores suspended in hypertonic solutions and supplied with two essential cations can be converted into viable protoplasts by lysozyme digestion of both cortex and germ cell wall. Calcium ions are necessary to prevent membrane rupture, and magnesium ions are necessary for changes indicative of hydration of the core, particularily the nuclear mass. Since remnant spore coat covered such protoplasts of Bacillus subtilis and the germ cell wall of B. cereus spores is not lysozyme digestible, coatless spores of B. megaterium KM were more useful for these studies. Lysozyme digestion in cation-free environment produced a peculiar semi-refractile spore core free of a cortex but prone to rapid hydration and lytic changes on the addition of cations. Strontium could replace Ca(2+) but Mn(2+) could not replace Mg(2+) in these digestions. When added to the spores, dipicolinic acid and other chelates appeared to compete with the membrane for the calcium needed for stabilization during lysozyme conversion to protoplasts. It is argued that calcium could function to stabilize the inner membrane anionic groups over the anhydrous dipicolinic acid-containing core of resting spores.


Formation of tumor-like structures on legume roots by Rhizobium. Tumor-like structures appeared on the roots of Medicago sativa, Alysicarpus vaginalis, and Trifolium pratense inoculated with a non-nodulating strain of Rhizobium trifolii or with irradiated cultures of either of two nodulating Rhizobium strains. The structures were composed of disorganized plant tissues which, on the basis of microscopic examination, were devoid of bacterial cells. Rhizobia which could nodulate legumes of one cross-inoculation group and which were able to induce formation of such tumor-like structures on plants of a second cross-inoculation group were isolated from extracts of these root growths. The apparent tumorogenic activity of some of the rhizobia, but not their nodulating capacity, was lost when the bacteria were transferred in laboratory media.


Isolation from the mushroom Agaricus bisporus and chemical synthesis of gamma-L-glutaminyl-4-hydroxybenzene.


Gamma-L-glutaminyl-3,4-benzoquinone. Structural studies and enzymatic synthesis.


Rabbit muscle adenosine 5'-monopbosphate aminohydrolase. Characterization as a zinc metalloenzyme.


Oxygenated form of protocatechuate 3,4-dioxygenase, a non-heme iron-containing dioxygenase, as reaction intermediate.


Experimental alteration of coupling resistance at an electrotonic synapse. Adjacent segments of the septate axon of the crayfish Procambarus are electrotonically coupled by junction located in the septa between them (see Pappas et al. 1970. J. Cell Biol.49:173). The coupling resistance at the septa was changed by several experimental treatments. Mechanical injury to an axon increased coupling resistance (more than 7-fold); no recovery of coupling resistance was observed, although the resting potential and resistance of the injured axon could return to near normal levels. Immersion in salines with Na propionate substituted for NaCl increased coupling resistance (mean: 6.1-fold). On return of the preparation to normal saline, coupling resistance recovered virtually completely. Immersion in low Ca(++) solutions moderately increased coupling resistance (3.5-fold or less), but return to normal saline was followed by large increases in coupling resistance (5-100-fold). 60 nM Ca(++) is near the maximum concentration that leads to increased coupling resistance on return to normal saline. Large increases in coupling resistance are associated with separation of junctional membranes (Pappas et al. 1970. Ibid.); calculations show that the separated membranes greatly increase in resistance. Increase in coupling resistance is probably an important response to injury. Mechanisms underlying changes reported here may be relevant to normal physiological processes of coupling and decoupling.


Diazepam in the treatment of childhood epilepsy.


Morphological correlates of increased coupling resistance at an electrotonic synapse. Close appositions between axonal membranes are present in the septum between adjacent axonal segments of the septate or lateral giant axons of the crayfish Procambarus. In sections the closely apposed membranes appear separated by a space or gap. The use of lanthanum indicates that there may be structures connecting the apposed membranes. The apparent gap is actually a network of channels continuous with the extracellular space. Adjacent axonal segments are electrotonically coupled at the septa. The coupling resistance is increased by mechanical injury of an axon, immersion in low Cl(-) solutions, and immersion in low Ca(++) solutions, followed by a return to normal physiological solution. Septa at which coupling resistance had been measured were examined in the electron microscope. The induced increases in coupling resistance are associated with separation of the junctional membranes (with the exception of the moderate increases during immersion in low Ca(++) solutions). Schwann cell processes are present between the separated axonal membranes. When nerve cords in low Cl(-) solutions are returned to normal physiological solution, coupling, i.e., electrotonic synapses. A model of an electrotonic synapse is proposed in which tween axonal membranes are again found. The association between the morphological and physiological findings provides further evidence that the junctions are the sites of electrotonic coupling, i.e., electrotonic, synapses. A model of an electrotonic synapse is proposed in which intercytoplasmic channels not open to the extracellular space are interlaced with a hexagonal network of extracellular channels between the apposed junctional membranes.


Monkey models for infectious hepatitis.

Monkey models for infectious hepatitis.


Evidence for duplication of the haemoglobin gamma-chain locus in Macaca nemestrina.


The monkey colony at Downe.

The monkey colony at Downe.


Changes in vasculature of the periodontium associated with tooth movement in the rhesus monkey and dog.


In vitro incorporation of [14C] L-leucine into monkey (Macaca irus) parotid and submandibular gland proteins.


The effect of various factors on the extraction of protein from the predominant microorganisms in human dental plaque.


Atavistic ocular ossicle.

Atavistic ocular ossicle.


The effect of surgical operation on thyroid activity.


Intramammary inoculation of mice with scrapie.


Clinical significance of 7s IgM in monoclonal IgM diseases. The sera from 117 patients with diseases associated with a high production of monoclonal IgM were analysed for the presence of low molecular weight (7s) IgM by using a simple thin-layer Sephadex technique. 7s IgM was found in the sera of patients with myelomata (66%), lymphomata (45%), and Waldenström's macroglobulinaemia (20%), but was absent from the sera of patients with benign monoclonal macroglobulinaemia.This technique provides a cheap and practical test which may be valuable in selecting patients with lymphomata from those with benign lesions.


The kinetics of the action of carbenicillin on Pseudomonas aeruginosa in plasma.


Coordinately bound calcium as a cross linking agent in elastin and as an activator of elastolysis.


Occupational and non-occupational factors associated with vesical neoplasm in Ireland.


Experimental modification of the chemistry and biology of the aging process.


The effects of various treatments on the surfaces of the cercariae and schistosomula of Schistosoma mansoni.


A new malaria parasite in Hylopetes alboniger Hodgson.


Properties of DNA from some malarial parasites.


Immunity of rats and mice following injection with 60Co irradiated Babesia rodhaini infected red cells.


Theileria parva: comparative infection rates of adult and nymphal Rhipicephalus appendiculatus.


Short-term culture of Plasmodium knowlesi.


Local production of antibody by the lactating mammary gland of the ewe and the effect of systemic immunization.


The fine structure of intra-erythrocytic stages of Nuttallia (Babesia) microti.


Multiplication of scrapie agent in mouse spleen.


The isolation and properties of nucleohistonefrom the fission yeast, Schizosaccharomyces pombe.


Isolation from Avena leaf tissues of a nuclease with the same type of specificity towards RNA and DNA. Accumulation of the enzyme during leaf senescence.


Pathophysiology of coronary circulation.


Pulmonary function at rest and during exercise following bagassosis.


Focal neurological manifestations in hypercalcemia.


Electro-encephalographic aspects of Lennox-syndrome.


A clinical note on a new antiepileptic 'Depakine'.


Immunochemical studies on pepsinogens A, C and D from the smooth dogfish, Mustelus canis.


Synthesis of 3'-deoxykanamycin effective against kanamycin-resistant Escherichia coli and Pseudomonas aeruginosa.


Antimetabolites produced by microorganisms. II. L-2-amino-4-pentynoic acid.


Area analysis of pressure-volume hysteresis in mammalian lungs.


Effects of sedation and posture on pulmonary gas exchange in the baboon.


A method for perfusion of cerebral ventricles of the conscious monkey.


Purification and properties of seminal vesicle ribonucleases.


Localization of cellulase components in Pseudomonas fluorescens var. cellulosa.


Relaxation of glycerinated muscle fibers and clearing response of myosin B in magnesium-inosine triphosphate medium.


Purification and properties of phosphotransacetylase from Lactobacillus fermenti.


Electron microscopic observations on growing and dividing protoplasts of Bacillus megaterium.


Comparison of galactokinase induction in protoplasts and intact bacteria of Bacillus megaterium 216.


Experimental middle ear homografts.

Experimental middle ear homografts.


An objective study of the effect of betahistine hydrochloride on hearing and vestibular function tests in patients with Ménière's disease.


Toxicological evaluation of carbon monoxide in humans and other mammalian species.


The first heart transplant in man: brief review with notes on a more recent case.


The use of ALG in 56 cases of heart transplantation.


In vivo testing of antihuman lymphocyte sera.


Prevention of infection after cardiac transplantation.


Blockade by polyphloretin phosphate of the prostaglandin F2a action on isolated human bronchi.


Quantitative estimation of human carbonic anhydrases B and C by electrophoresis in antibody containing agarose.


Galactosemia: evidence for a structural gene mutation. Identical immunoprecipitin reactions appeared in double immunodiffusion between rabbit anltibodies to human galactose-1-phosphate uridyl transferase and red cell preparations from both normal and galactosemic individuals. The galactosemic erythrocyte preparations quantitatively absorbed the antiboy that immunoprecipitates enzymatically active galactose-1-phosphate uridyl transferase.


About boxing: review of historical and medical aspects.


IV. Detection and differentiation of M-proteins by the acid-gel reaction--a re-evaluation.


Myrmicacin, the first insect herbicide.

Myrmicacin, the first insect herbicide.


Blood coagulation and fibrinolysis.

Blood coagulation and fibrinolysis.


Microbiological determination of deoxyribose derivatives in urine.


Therapy of Hodgkin's disease.

Therapy of Hodgkin's disease.


Biosynthesis of monkey growth hormone and prolactin in vitro.


Radioimmunoassay for rhesus monkey luteinizing hormone(LH) with anti-ovine LH serum and ovine LH-131-I.


Thyrotropin releasing hormone (TRH) stimulation of thyroidal radioiodine release in the rat: comparison between intravenous and intraventricular administration.


Sensitization to the enzymes in detergents.


The Australia (hepatitis-associated) antigen in fibrinogen and other fractions of human plasma. Human plasma containing the Australia (hepatitis-associated) antigen was fractionated by the cold ethanol method of Cohn, Strong, Hughes, Mulford, Ashworth, Melin, and Taylor (1946) and small aliquots were examined for the presence of this antigen by immunodiffusion and by electron microscopy. The findings were in general agreement with the postulated risk of transmitting hepatitis by blood derivatives. The Australia (hepatitis-associated) antigen was detected in fibrinogen, thrombin, and antihaemophilic globulin as well as in other fractions. The antigen was not found in gamma globulin (immunoglobulin fraction) nor in albumin.The use of radioiodinated fibrinogen for the diagnosis of deep vein thrombosis is discussed and it is concluded that the use of fibrinogen for diagnostic procedures should be assessed against the possible risk of hepatitis.


Principles of protein estimation by radial immunodiffusion.


Comparison of three serological methods for the detection of hepatitis-associated antigen. Serum hepatitis (Australia) antigen in the sera of hepatitis patients and carriers can be detected in one and a half to three hours by crossover electrophoresis. The method is more sensitive than the immunodiffusion technique commonly employed in this field. It is of the same order of sensitivity as complement fixation but is less complicated.Crossover electrophoresis is thus the method of choice for the rapid screening of sera for hepatitis antigen; complement fixation may be used for quantitative determination of antigen in positive cases.


DNA strand reassociation and polyribonucleotide binding in the African green monkey, Cercopithecus aethiops.


Tick-borne typhus (Rocky Mountain spotted fever). Epidemiologic trends, with particular reference to Virginia.


Pulp response in the rhesus monkey to "composite" dental restorative materials in unlined cavities.


Effects of a sclerosing agent on odontogenesis.


Pseudopericarditis in diabetic ketoacidosis.


Diverticulum of the left ventricle in a child with fatal cerebral embolization.


In vitro studies of 100 strains of meningococci: clinical implications.


Longitudinal growth of the lumbar articular processes with reference to the development of clefts.


Effects of ouabain on splanchnic hemodynamics in the rhesus monkey.


Distribution of labelled calcium-, yttriumand chromium-chelates of EDTA in rats.


Plurimodal heterogeneity of base composition of DNA isolated  from Bacillus subtilis.


Isolation and identification of a new toxin, altenuene, from the fungus Alternaria tenuis.


Contact dermatitis. 8. The significance of nickel sensitivity in women.


Chemical markers of transplantation individuality solubilized with sonic energy.


Formation of biologically active substances by rhizosphere bacteria and their effect on plant growth.


Quantitative immunology of immune hemolytic anemia. I. The fixation of C1 by autoimmune antibody and heterologous anti-IgG antibody. The fixation of the first component of complement (C[unk]1) by rabbit and goat anti-IgG antibodies reacting with auto- or isoimmune antibodies attached to red cells has been investigated. Two molecules of the rabbit IgG anti-IgG were required to fix a single molecule of C[unk]1, whereas only one molecule of goat IgM anti-IgG was required. The relationship between the number of auto- or isoimmune antibody molecules attached to the red cells and the amount of C[unk]1 fixed by anti-IgG was determined by the concentration of anti-IgG. A concentration of anti-IgG was found such that the number of molecules of C[unk]1 fixed was directly proportional to the concentration of auto- or isoimmune antibody. By this method a sensitive, reproducible minimum estimate of the amount of cell-bound and serum antibody could be made.


Study on the glutamylpolypeptide antigen in the genus Bacillus. I. Preparation of D-glutamylpolypeptide by gel filtration.


Intraluminal duodenal diverticulum.

Intraluminal duodenal diverticulum.


Hemoglobin A2. What is it for?

Hemoglobin A2. What is it for?


Cats and childhood leukemia.

Cats and childhood leukemia.


Serum immunoglobulin D level in healthy adults in Thailand.


Skin test for medullary thyroid carcinoma.


Amyloid induction with casein in mice of different ages and investigation for casein antibodies using the single radial diffusion technique.


Center-surround balance in receptive fields of cells in the lateral geniculate nucleus.


The biosynthesis of thiamine. A yeast enzyme system which converts 2-methyl-4-amino-5-aminomethylpyrimidine to 2-methyl-4-amino-5-hydroxymethylpyrimidine.


Mitochondrial autonomy. Studies on the mitochondrial glycoprotein: glucosyl and mitochondrial glycoprotein: mannosyl transferase enzyme systems of rat liver mitochondria utilizing endogenous acceptors.


Automated immunoprecipitin technique for analysis of the third component of complement (C'3) in human serum.


Family studies in individuals with selective absence of gamma-A-globulin.


Polyclonal immunoglobulin deficiency in myelomatosis and macroglobulinaemia.


Antibodies to brain and other tissues in cases of Mycoplasma pneumoniae infection.


Purification and some properties of alkaline phosphatase from intestinal mucosa of the eel adapted to fresh water or sea water.


Use of plasma instead of serum in laboratory tests for infectious mononucleosis. Until recently, the exclusive use of serum in all laboratory tests for detecting heterophile antibody has remained unquestioned. This communication describes a series of studies which demonstrate that comparable results are obtained when sequestrenized plasma is used instead of serum in the conventional differential absorption technique and in two slide screening tests. It is confirmed that significant differences in the final differential absorption titres can result from variations in the agglutinability of erythrocytes from different sheep but such differences when present are the same for plasma as for serum. The advantages of using plasma for routine purposes are briefly discussed.


Metabolic alterations in diabetes mellitus.


Acute management of diabetic ketoacidosis.


Chronic management of diabetes in children.


Cytotoxicity of filtrates of haemolytic Escherichia coli.


Ultrastructural characteristics of mycobacterial growth.


The fate of ingested Pseudomonas aeruginosa in normal persons.


Malignant carcinoid of the Ampulla of Vater.


A test with brucella antigen and antisera for assessing the titre of fluorescein conjugates of anti-human-globulin.


Long-term hyperalimentation in ulcerative colitis.


Nitroglycerin tablets: loss of potency.

Nitroglycerin tablets: loss of potency.


Pheromones: isolation of male sex attractants from a female primate. Fractionation of vaginal secretions from rhesus monkeys by partitioning and chromatographic procedures, combined with behavioral studies, demonstrates that short-chain aliphatic acids are responsible for stimulating the sexual behavior of males. Injection of estradiol into ovariectomized females increases the concentration of volatile acids in secretions which will then sexually stimulate these male primates.


The ultrastructure of nuclear division in a suctorian, Tokophrya infusionum.


Ballantyne and triple edema.

Ballantyne and triple edema.


High oxygen effect for the release of enzymes from isolated mammalian lysosomes after treatment with ionizing radiation.


Role of spontaneous abortion in Rh immunization.


Diagnosis of fetal hemolytic disease by a chemical test in a case with metheme-contaminated amniotic fluid.


Evaluation of first morning urine to detect significant bacteriuria. I.


Variability of naturally occurring antihuman placental lactogen dependent upon preparations tested.


Maternal placental and myometrial blood flow of the rhesus monkey during uterine contractions.


Management of upper gastrointestinal fistula with a double lumen tube.


Total intravenous nutrition. An adjunct to the management of infants with a ruptured omphalocele.


Cholesterol gallstones and the chemical composition of bile in baboons.


Chromatid structure: relationship between DNA content and nucleotide sequence diversity.


Micro-isoelectric focusing of proteins in pilocarpine-induced sweat.


Serum immunoglobulins in hydatid disease.


Lytic enzyme from lysates of Streptomyces venezuelae infected with actinophage MSP2. A lytic enzyme was purified 600-fold with 12% recovery from lysates of Streptomyces venezuelae S13 infected with actinophage MSP2. The purified enzyme preparation was homogeneous as shown by polyacrylamide electrophoresis. The enzyme was active over a pH range 6.0 to 9.0 with a maximum at pH 7.5. The pH profile for stability was sharp, with an optimum at pH 7.5. Maximal activity occurred between 30 and 35 C. The enzyme was stable at 20 C or less. A 30-min exposure to 25, 30, 35, 40, 45, and 50 C produced an inactivation of 3, 40, 77, 82, 93, and 100%, respectively. Lytic activity was stimulated fivefold by either 5 x 10(-3)m Mg(2+) or Mn(2+) and three- and twofold by Ca(2+) and Ba(2+), respectively. Addition of Na(+), K(+), NH(4) (+), or Li(+) to the tris(hydroxymethyl)aminomethane-hydrochloride buffer did not alter the rate of lysis. Enzyme activity was inhibited 74 and 27% by 10(-4) and 10(-5)m ethylenediaminetetraacetic acid (EDTA), respectively. The inhibition by EDTA was reversed partially by addition of Mg(2+). Lytic activity was abolished by either 5 x 10(-4)m HgCl(2) or p-hydroxymercuribenzoate, whereas 5 x 10(-4)m CuSO(4) inhibited 72%. Cell wall solubilization paralleled the release of N-terminal amino groups and reached a level of 0.23 mumole per mg of cell walls. No release of reducing power was detected in treated or untreated cell wall suspensions. Tests for proteolytic activity were negative.


Purification of Bacteroides amylophilus protease. A protease was released by Bacteroides amylophilus cells in late stationary phase, approximately 12 hr after maximum cell density was reached. The protease was concentrated by adsorption on diethylaminoethyl (DEAE)-Sephadex and was purified 532-fold by DEAE-Sephadex chromatography, by G-200-Sephadex gel filtration, and by isoelectric focusing. The purified protease was active between pH 4.5 and 12.0 with optima at pH 6.0 and 11.5. Evidence against there being a single protease was given by the differential inhibition of esterase and protease activities by some inhibitors. There was some evidence that only a single protease was present as the ratio of protease activity at various pH values did not alter significantly during purification or when the purified protease was partially heat-inactivated or treated with two specific trypsin-type protease inhibitors: N-alpha-tosyl-l-lysylchloromethyl ketone or phenylmethane-sulfonyl fluoride. Two forms of the same protease were found by acrylamide gel electrophoresis. Gel filtration confirmed the presence of protease in 30,000 and 60,000 molecular-weight forms. Treatment with 1 mm ethylenediaminetetraacetic acid or with 4 m urea failed to convert the 60,000-molecular-weight to the 30,000-molecular-weight species.


Histidinol dehydrogenase (his D) mutants of Salmonella typhimurium. A multidisciplinary analysis has been applied to over 150 hisD mutants of Salmonella typhimurium in a study of gene-enzyme relationship. The mutants were examined for production of immunologically cross-reacting material by using antibody to purified histidinol dehydrogenase, and for genetic complementation by using a set of F' factors bearing Escherichia coli hisD complementing mutants. Classifications as to missense, nonsense, frameshift, or deletion mutant are proposed on the basis of mutagenesis and suppression tests. For the suppression tests the mutants were examined both by a simultaneous suppression technique and by testing for response to E. coli F' factors bearing a recessive lethal amber and a recessive lethal ochre suppressor. The data are interpreted in relation to the position of the mutations in the recombination and complementation maps and in relation to the known composition of histidinol dehydrogenase. The gene hisD appears to be single cistron for the production of a single biosynthetic polypeptide.


Control of diaminopimelate decarboxylase by L-lysine during growth and sporulation of Bacilluscereus. l-Lysine caused repression of diaminopimelate decarboxylase synthesis in Bacillus cereus when grown in either a minimal defined medium (CDGS medium) or a complex defined medium (a modified lysine assay medium). When cells were grown in either of the two media, variations in the specific activity of the enzyme as a function of time were found to be correlated with the intracellular lysine pool size during growth. From all of the data presented, it seems reasonable to conclude that during growth the synthesis of diaminopimelate decarboxylase is probably regulated by the intracellular lysine pool size. The relationship between lysine pool concentration and the specific activity of the enzyme did not occur in sporulating cells. The specific activity of diaminopimelate decarboxylase started to decrease at the end of exponential growth and continued to decline until it became nondetectable at the time of dipicolinic acid synthesis and development of spore refractility. Throughout this time, the intracellular lysine pool size remained below that which allowed derepression of enzyme synthesis during exponential growth. The mechanism(s) responsible for the observed decrease in the specific activity of the enzyme at the end of exponential growth is unknown. A threefold rise in the intracellular diaminopimelic acid concentration occurred when there was little or no detectable enzyme activity at the time of dipicolinic acid synthesis. This accumulation of diaminopimelic acid may exert positive control on the synthesis of spore peptidoglycan, the major component of the spore cortex.


Extracellular transglucosylase and alpha-amylase of Streptococcus equinus. Culture filtrates of Streptococcus equinus 1091 contained alpha-amylase and transglucosylase. The effects of calcium carbonate, age of inoculum, concentration of maltose, and duration of the fermentation on alpha-amylase and transglucosylase production were determined. The extracellular alpha-amylase was purified 48-fold and was free of transglucosylase activity. The alpha-amylase (amylose substrate) required Cl(-) for maximum activity; ethylenediaminetetraacetic acid (EDTA) partially inhibited activity, but CaCl(2) prevented EDTA inhibition. The temperature optimum was 38 C at pH 7.0, and the pH optimum was 7.0 at 37 C in the presence of CaCl(2). Predominant final products of amylose hydrolysis, in order of decreasing prevalence, were maltose, maltotriose, maltotetraose, and glucose. The alpha-amylase showed no evidence of multiple attack. The extracellular transglucosylase was purified 27-fold, but a small amount of alpha-amylase remained. Transglucosylase activity (amylose substrate) was not increased in the presence of CaCl(2). The temperature optimum was 37 C at pH 6.5, and the pH optimum was 6.0 at 37 C. Carbohydrates that served as acceptors for the transglucosylase to degrade amylose were, in order of decreasing acceptor efficiency: d-glucose, d-mannose, l-sorbose, maltose, sucrose, and trehalose. The extracellular transglucosylase of S. equinus 1091 synthesized higher maltodextrins in the medium when the cells were grown in the presence of maltose.


Purification and properties of L-asparaginase from Serratia marcescens. The purification and properties of a tumor inhibitory l-asparaginase from Serratia marcescens are described. The following properties of the enzyme were examined: kinetics of the enzyme reaction, catalytic activity as a function of pH, boundary sedimentation velocity, electrophoresis on polyacrylamide gel, immuno-electrophoresis against homologous and heterologous antisera, immunodiffusion, blood clearance rate in mice, and inhibition of the 6C3HED lymphoma in C3H mice. Complete regression of this tumor was obtained with a smaller dose of the enzyme from S. marcescens than with enzyme from Escherichia coli. The reason for this difference was not evident from a comparison of several properties of the two enzymes.


Physical linkage between 5 s, 16 s and 23 s ribosomal RNA genes in Bacillus subtilis.


Morphological changes and antibiotic-induced thermal resistance in vegetative cells of Bacillus subtilis. The morphology and thermal resistance of vegetative cells of Bacillus subtilis W168 were examined after growth at 37 and 53 C. Vegetative cells grown at 37 C exhibited a typical trilaminar morphology, whereas cells grown at 53 C exhibited a cell wall which was apparently thicker and more loosely organized and had a poorly defined periphery. A concurrent increase in thermal resistance to a heat shock of 60 C occurs with the change in cell wall morphology. The change to the aberrant cell wall form, or its reversal to the normal form, is always accompanied by the gain or the loss of thermal resistance, respectively. The inhibition of protein synthesis by chloramphenicol has little effect upon the acquisition of thermal resistance at 53 C. Addition of the disaccharide pentapeptide subunit to the cell wall peptidoglycan is apparently essential to growth at 53 C and the acquisition of thermal resistance, since both growth and thermal resistance are inhibited by bacitracin. Two antibiotics, penicillin and cycloserine, which inhibit the final cross-linking of the cell wall peptidoglycan at two separate points, do not affect the acquisition of thermal resistance at 53 C. These same antibiotics induce a high degree of thermal resistance at 37 C. It is proposed that a change in the cell wall structure is related to an increased thermal resistance.


Suppression by derepression in threonine dehydratase-deficient mutants of Bacillus subtilis. Partial suppressors of isoleucine-requiring mutants (Ile(-)), deficient in threonine dehydratase (TD), were studied. The suppression enables these auxotrophs to grow on the precursors homoserine or threonine as well as isoleucine. There are two genetically distinct classes of these suppressors: sprB, linked to threonine mutational sites, and sprA which is not linked to the Ile or Thr genetic region. SprA leads to the appearance of a low level of TD activity (2 to 4% of wild type) compared to <0.2% in Ile(-) mutants. This new TD activity is not activated by glutathione or adenosine monophosphate. Unlike the major TD, it is insensitive to inhibition by isoleucine and it is not coordinately controlled with dihydroxy acid dehydratase. Another class of auxotrophs, containing no suppressor mutation, can grow on isoleucine and homoserine or threonine because they possess structural gene mutations located near the terminus of the TD gene that result in partial impairment of TD. This TD activity (2% of wild type) is end product inhibited by isoleucine to a lesser extent than wild type TD. In this and a following paper, evidence is presented that sprA acts by derepressing a minor TD activity encoded within the threonine synthetic region. The major TD gene is separated from the region encoding the enzymes of threonine biosynthesis by 73% of the chromosome. Like the major TD, the minor TD catalyzes the first reaction in the conversion of threonine to isoleucine and is therefore closely related biochemically and genetically to the enzymes of the threonine synthetic region.


Kinetics of labeling of fast-renaturing DNA in Bacillus subtilis.


Immunological results of hepatic assistance procedures.


Studies on the antitumor activity, mechanism of action, and cell cycle effects of camptothecin.


A fetal salvage program: the use of plasma diamine oxidase as a fetal monitor.


Antigenicity of human choriocarcinoma.

Antigenicity of human choriocarcinoma.


Control of transcription in bacteria.

Control of transcription in bacteria.


RNA of Australia antigen.

RNA of Australia antigen.


The critically ill child: diabetic ketoacidosis and coma.


Comparison of late results of treatment in surgically treated patients with pulmonary tuberculosis and those who refused surgery. I. Period from 1950 to 1959.


Studies on mycobacteriophages. Lytic properties of mycobacterophages 33-D isolated from lysogenic bacilli.


Caseation of lymph nodes in infants after intracutaneous BCG vaccination with original French vaccine.


Cardiac rate regulated by nutritional factor in young rats. Milk fed by stomach tube to 2-week-old rats separated from their mothers without feeding for 16 hours transiently but fully reversed the decrease in cardiac rate which had occurred during separation. This effect was rapid in onset and was related to dosage; it was not dependent upon gastric distention, but did depend upon beta-adrenergic transmission.


Urea cycle enzyme adaptation to dietary protein in primates. Activities of the five hepatic enzymes of the urea cycle increased two- to threefold in monkeys on a 60 percent protein diet compared with levels in monkeys on an isocaloric 6 percent protein diet. Equal adaptation occurred in rats on the same diets. These enzyme activities in adult humans approximate those of monkeys on a similar protein intake.


Lac repressor.

Lac repressor.


Bacteriologic and clinical evaluation of minocycline, a new tetracycline.


Therapeutic effectiveness of synthetic thyroxine-triiodothyronine mixtures.


A rationale for studying analgesia: a double-blind study in postpartum patients.


A double-blind study of an oxazepam hemisuccinate-hexadiphane combination in management of gastrointestinal disorders.


An evaluation of metiapine in chronic schizophrenia.


An unusual re-evaluation of marsilid as an antidepressant.


Revascularization and bone healing after posterior maxillary osteotomy.


Heterotransplantation of cultured cell lines in newborn hamsters treated with antilymphocyte serum.


Crystallization of a second adenovirus protein (the fibre).


Autologous bone marrow transplantation in irradiated baboons.


A simple method for the purification of the 6-phosphogluconate dehydrogenase from Candida utilis.


Counter telescope measurements of a beam of negative pi mesons.


The role of nutrition in the adaptation of the small intestine after massive resection.


Retrorectal tumors in adults.

Retrorectal tumors in adults.


Hepatic ultrastructure in chondroitin-4-sulfate mucopolysaccharidosis.


Hydrocortisone: inhibition of DNA synthesis and mitotic rate after local application to mouse epidermis.


Putrescine and polyamines in relation to nucleic acids in mouse liver after partial hepatectomy.


Joint FAO-WHO Expert Committee on Brucellosis. Fifth report.


A comparison of the immune response of tonsils with the appendix and spleen in neonatal rabbits.


Effects of oestrogens on serum thyroxine-binding globulin capacity and on the peripheral metabolism of thyroxine in patients with hepatic cirrhosis.


Microflocculation assay for gonococcal antibody. An antigen suspension consisting of cholesterol-lecithin particles sensitized with an extract of gonococci was used in a flocculation assay for the detection of human antibodies to Neisseria gonorrhoeae. With culturally and clinically diagnosed cases of uncomplicated gonorrhea, sera from 70 (49.6%) of 141 males and 207 (79.0%) of 262 females were reactive in the assay. Sera from four (7.3%) of 55 males and 11 (5.4%) of 203 females, all presumed normal, were reactive. The assay utilized equipment available in most clinical and public health laboratories.


Resistance to D-cycloserine in the tubercle bacilli: mutation rate and transport of alanine in parental cells and drug-resistant mutants. A single transport system was found to accumulate l- and d-alanine, glycine and d-serine in Mycobacterium tuberculosis. The results of inhibition experiments suggested that the antibiotics d-cycloserine and O-carbamyl-d-serine were also transported by the alanine-glycine-d-serine system. A d-cycloserine-resistant permease-competent (d-CS(r)/perm(+)) mutant and a d-cycloserine-resistant permease-defective (d-CS(r)/perm(-)) mutant were isolated. The d-CS(r)/perm(-) mutant was not found to be more resistant to the drug than was the d-CS(r)/perm(+) mutant. The data were consistent with the conclusion that resistance to d-cycloserine in the tubercle bacilli is primarily due to mutations in the gene(s) controlling the enzyme d-alanyl-d-alanine synthetase. The mutation rate was calculated to be about 10(-10) mutations per bacterium per generation.


Antibiotic susceptibility patterns of recent isolates of Corynebacterium diphtheriae. A variety of factors which might affect zone sizes were studied with strains of Corynebacterium diphtheriae; a standard disc method for antimicrobial sensitivity testing was used. Moderate variations in inoculum size, inoculum preparation, and pH of Mueller Hinton agar (MHA) did not appreciably affect zone sizes. The addition of blood to MHA was necessary to insure the growth of all C. diphtheriae strains on all lots of MHA. Zone diameters on MHA with blood were consistently 4 to 9 mm smaller than on plain MHA; however, zone diameters were within the sensitive range for seven antibiotic discs used on both media. Minimal inhibitory concentration (MIC) values for penicillin, erythromycin, and rifampin were determined by a plate dilution method. The geographical source, toxigenicity, and type of the strains showed no significant correlation with MIC values or zone diameters for eight antibiotic discs. When MIC values were compared to obtainable blood levels, all of the strains appeared to be sensitive with MIC values of </= 0.5 mug/ml for penicillin and </= 0.01 mug/ml for erythromycin and rifampin. Strains from persons who failed to have C. diphtheriae eliminated by one course of treatment with penicillin or erythromycin were examined. The susceptibility of these strains did not vary significantly from that of the other strains studied.


Contamination of sputum induction equipment during patient usage. Sputum induction equipment was evaluated for its capacity to become contaminated by patients harboring Mycobacterium tuberculosis. The mouthpiece, goose-neck, and 10% NaCl solution were found capable of being contaminated by tuberculous patients. Two per cent glutaraldehyde was shown to be an effective means of decontamination. This study indicates that the entire induction apparatus must be cleaned and decontaminated between patients.


Nonspecific precipitation of serum proteins by sodium lauryl sulfate in agar diffusion and immunoelectrophoresis. The anionic detergent sodium lauryl sulfate (SLS), in a final concentration of 0.1% and greater, reacted with whole serum in agar diffusion and immunoelectrophoresis to form artifactual precipitin lines. These lines occurred when either Ionagar or agarose was used as the supporting gel and were not affected by the presence of urea and 2-mercaptoethanol. Analytic chemical tests confirmed that the precipitating agent is SLS, and staining techniques showed that the detergent precipitates both protein and lipoprotein components of whole serum. Multiple artifactual precipitin lines occurred with a wide variety of animal sera, and a single line formed with human 7S immunoglobulin. Hence, in agar diffusion studies in which SLS is present in the test system, these artifactual lines may be easily misinterpreted as true antigen-antibody precipitin reactions.


Kinetics of the vaccinia virus plaque neutralization test. Neutralization of vaccinia virus with immune rabbit serum occurred optimally when incubated at 37 C for 16 to 24 hr in the plaque neutralization test employing the MA-104 embryonic rhesus monkey kidney cell line.


The tyrosinases of mouse melanoma. Isolation and molecular properties.


Snake venom toxins. Purification and properties of three toxins from Naja nivea (Linnaeus) (Cape cobra) venom and the amino acid sequence of toxin delta.


Purification of rat heart and rat liver citrate synthases. Physical, kinetic, and immunological studies.


The stability of messenger ribonucleic acid during sporulation in Bacillus subtilis.


Isoantibodies to human IgM: serologic and immunochemical investigations.


Specificity and immunoglobulin characteristics of autoantibodies in acquired hemolytic anemia.


Hapten binding studies on mouse IgA myeloma proteins with antibody activity.


Primate respiratory mast cells. Reactions with Ascaris antigen and anti-heavy chain sera.


Hypersensitivity to ascaris antigens. VI. Physicochemical, immunochemical and biologic properties of rabbit homocytotropic antibodies produced to crude extracts.


X-ray study of competence development in Bacillus subtilis.


A biological assay for intracellular SPP1 DNA.


Teratologists united.

Teratologists united.


Ultraviolet light: a new stimulus for the induction of platelet aggregation. Exposure to ultraviolet light (253.7 nanometers) causes mammalian blood platelets to aggregate. Aggregation is markedly enhanced in the presence of extracellular fibrinogen and is followed by the grodual of relatively small amounts of nucleotide and serotonin. Aggregation is inhibited by ethylenediamine tetraacetic acid or a combination of 2-deoxy-D-glucose and antimycin A. Adenosine, apyrase, and prostaglandin E(1) produced slight inhibition. The effect of exposure to ultraviolet light is cumulative and lasting. This agent may be used to study the process of platelet aggregation after the removal of the stimulus, by delaying the addition of fibrinogen until after cessation of irradiation. Thus ultraviolet light is the first agent known which may be used to study platelet aggregation in a period following its removal.


An outbreak of encephalomyelitis in broiler rabbits caused by Nosema cuniculi.


Some further observations on the iodine reduction test as an aid in the diagnosis of swine fever.


An ultrastructural study of sites of granule extrusion in the anterior pituitary of the rat.


Studies on the complement fixation test with Mycoplasma pneumoniae antigen. 6. Preparation of cell antigens with low contents of medium constituents.


An immunodiffusion micromethod for comparative analysis of the hemolytic activity and antigenicity of streptolysin O.


The formation of streptolysin O under stabilized pH conditions.


Bilirubin transport by liver in adult Macaca mulatta.


Intracellular polysaccharide production by human oral strains of Lactobacillus casei.


Axial tomography of the orbits and paranasal sinuses.


Value of vaginal and rectal cultures in the diagnosis of gonorrhoea. With special reference to areas with limited medical facilities.


PAM plus probenecid and procaine penicillin plus probenecid in gonorrhoea.


Treatment of gonorrhoea with single oral doses. Results obtained with doxycycline or rifampicin compared with intramuscular penicillin.


The chemical synthesis of two isomers of glucosaminylphosphatidylglycerol.


Rapid method for protein quantitation by electroimmunodiffusion.


The effects of gonadotropin immunization of prepubertal rabbits on gonadal development.


In vitro labelling of heparin with chromium-51.


Observations on the functional internal anal sphincter of the vervet monkey.


Pharmacokinetics of halothane in man: a novel approach.


Free fatty acid production in cerebral white and grey matter of the squirrel monkey.


The occurrence of open-bite in the monkey: an observation.


Problems in the serological typing of IPN virus.


Acid to base phosphorylation and membrane integrity in plastids of greening maize.


Laser-induced reactions of P700 and cytochrome f in a blue-green alga, Plectonema boryanum.


3-(3,4-dichlorophenyl)-1, 1-dimethylurea-insensitive oxygen production in a cell-free preparation from Phormidium luridum that shows redox potential dependent coupling to one-electron oxidants.


Respiratory tract damage in burns. Case reports and review of the literature.


Competence for genetic transformation and the release of DNA from Bacillus subtilis.


New-style cold abscesses.

New-style cold abscesses.


Clinical aspects of oculo-auriculo-vertebral dysplasia.


The treatment of carcinoma of the bladder by external irradiation. A clinical trial. II.


Ultrastructural changes in the acrosome and sperm membranes during maturation of spermatozoa in the testis and epididymis of the rabbit and monkey.


Comparative ultrastructure of the mammalian amnion.


Amniocentesis of the sensitized Rh-negative patient. A combined study in 2 community hospitals.


Enzymatic characterization of a mutant of Escherichia coli with an altered DNA ligase. A temperature-sensitive, radiation-sensitive mutant of Escherichia coli has been assayed for DNA ligase activity in vitro. The strain contains a markedly reduced amount of DNA-joining activity, which is thermolabile. The formation of the ligase-adenylate intermediate is also temperature-sensitive in vitro. Two temperature-resistant revertants of the mutant contain normal amounts of a thermostable ligase. The mutant is killed by growth at 42 degrees C, a temperature at which it displays aberrant DNA synthesis. These results suggest that the ligase is necessary for normal DNA metabolism and viability in this strain.


A new photosynthetic pigment, "P430": its possible role as the priary electron acceptor of photosystem I. The technique of flash kinetic spectrophotometry was used to demonstrate a broad absorption band around 430 nm, which was kinetically different from P700, in several photosystem-I particles from spinach and blue-green algae. The component represented by this absorption band, designated as "P430", was bleached as fast as P700. Its recovery in the dark was accelerated by ferredoxin and by various artificial electron acceptors with redox potentials as low as -521 mV. The recovery kinetics have been demonstrated to be identical to those of a concomitant reduction of several of the artificial electron acceptors used. Tentatively, "P430" has been proposed as a possible primary electron acceptor of photosystem I.


A form of cytochrome b5 that contains an additional hydrophobic sequence of 40 amino acid residues. A species of cytochrome b(5) with a monomer molecular weight of 16,700 has been isolated from rabbit-liver microsomes by a procedure that uses detergents and avoids the use of any proteolytic or lipolytic enzymes. This detergent-extracted cytochrome b(5) is larger than the trypsin- or lipase-extracted enzyme, and appears to contain an extremely hydrophobic appendage of 40 amino acids, probably at the N-terminus. The hydrophobic character of the extra amino acid sequence leads to aggregation in the absence of detergents, and may be of considerable importance in the binding of the enzyme to microsomes. It is suggested that the hydrophilic portion of the cytochrome molecule, which bears the heme and is enzymatically functional, is oriented toward the surface of the membrane where it readily reacts with nonmicrosomal proteins, while the hydrophobic "tail" anchors the heme protein tightly to the membrane.


An enzyme-based theory of obligate anaerobiosis: the physiological function of superoxide dismutase. The distribution of catalase and superoxide dismutase has been examined in various micro-organisms. Strict anaerobes exhibited no superoxide dismutase and, generally, no catalase activity. All aerobic organisms containing cytochrome systems were found to contain both superoxide dismutase and catalase. Aerotolerant anaerobes, which survive exposure to air and metabolize oxygen to a limited extent but do not contain cytochrome systems, were found to be devoid of catalase activity but did exhibit superoxide dismutase activity. This distribution is consistent with the proposal that the prime physiological function of superoxide dismutase is protection of oxygen-metabolizing organisms against the potentially detrimental effects of the superoxide free radical, a biologically produced intermediate resulting from the univalent reduction of molecular oxygen.


Detection of Australia antigen by radioimmunoassay. A double-antibody radioimmunoassay has been developed for the detection of Australia antigen and antibodies to Australia antigen in human sera. The assay is simple to perform, and can be completed in 18 hr. Based on parallel studies of human sera, it is approximately 2000 times more sensitive than agar gel diffusion and 200 times more sensitive than quantitative complement fixation. Australia antigen or antibody to Australia antigen was demonstrated in 22 of 23 single serum samples obtained from patients acutely ill with the long-incubation form of hepatitis; eight of the sera were negative when evaluated by less sensitive techniques.


Allotypy of high density lipoprotein of rabbit serum. A common antigenic polymorphism of high density lipoprotein (HDL) in rabbit serum is described. The presence or absence of an antigen termed Hl 1 appears to be controlled by autosomal dominant inheritance. The polymorphism should be a useful tool in the study of serum lipoproteins, particularly since genetic polymorphisms within the low density lipoprotein are already known in several species. The Hl polymorphism may make the rabbit more useful for model studies of serum lipoproteins in health and disease.


Three-dimensional molecular models of bacterial cell wall mucopeptides (peptidoglycans). Molecular models have been built of mucopeptides (peptidoglycans) from various species of bacteria. If pairs of glycan chains are hydrogen-bonded as are those of chitin, the carboxyl groups of muramic acid are so oriented that a regular three-dimensional structure can be built. Helical conformation of the peptides is not likely, but pseudo-beta conformation gives the possibility of multiple interpeptide hydrogen bonding. Calculations of the expected dimensions of the cell wall from the model for mucopeptide give results of the right order for bacilli. The effect of the configuration of the amino acids on the conformation of the peptide has been examined.


Fate of transforming DNA after uptake by competent Bacillus subtilis: failure of donor DNA to replicate in a recombination-deficient recipient. The fate of radioactively-labeled transforming DNA was studied in a recombination-deficient strain of Bacillus subtilis that carried the recB2 mutation (Rec(-)) and was sensitive to radiation. Experiments performed with extracts of this strain after transformation showed that the recovery of donor transforming activity and the appearance of recombinant transforming activity occurred to the same extent as in the Rec(+) strain. Sucrose gradient analyses revealed that donor-recipient complex is also formed to the same extent in the Rec(-) and Rec(+) strains, but that 80-90% of the donor genetic material in the complex failed to replicate in the Rec(-) mutant.


Detection of the scrapie agent in tissues of normal mice with special reference to the possibility of accidental laboratory contamination.


Masking tape as a protection for the cold storage of blood films for indirect fluorescent antibody tests.


Serological evidence for the identification of Babesia major in Britain.


More doctors--how are we going to get them?


Fatal juvenile polyposis in infancy.

Fatal juvenile polyposis in infancy.


Repeatability estimates of some economic characters in Italian buffaloes.


The foetal membranes and fluids in the Egyptian buffalo.


Hypothalamic control of pituitary thyrotrophic hormone, of cows and buffaloes during the estrous cycle.


Seasonal and sexual variations of certain constituents of normal camel blood.


The mystery of "non-nucleated plates" in the alveolar epithelium of the lung explained.


Biosynthesis of erythromycin C by Streptomyces erythreus.


The functioning of the membranes of the cells of mesophilic and thermophilic varieties of Bacillus megaterium.


Comparison of the effect of natural and synthetic humates and EDTA on the growth of Escherichia coli.


Gene frequencies and microdifferentiation among the Makiritare Indians. I. Eleven blood group systems and the ABH-Le secretor traits: a note on Rh gene frequency determinations.


Antimicrobial activity of sodium n-alkylsalicylates. The activities are reported of sodium salts of several n-alkylsalicylic acids against Staphylococcus aureus, Pseudomonas aeruginosa, Mycobacterium phlei, and Candida albicans. The acids had alkyl substituents of 1 to 18 carbon atoms in the 3, 4, or 5 positions relative to the carboxyl group of salicylic acid. Generally, the antimicrobial properties were typical of anionic surface-active agents although the salicylates are more potent than most of these, particularly against S. aureus.


Methods for accelerating the fluorescent-antibody test for rabies diagnosis. The time required to perform the fluorescent-antibody test for rabies was reduced by eliminating acetone fixation of the brain impressions and by incubating the conjugate-impression reaction at room temperature for only 10 min. Elimination of the preliminary acetone fixation had no effect on the diagnosis of impression smears from 246 mammalian brains by immunofluorescence. Staining at 37 C for 30 min and staining at room temperature for 10 min were found to be equally effective in the examination of impression smears from 161 brain samples. The procedure, as modified, shortens the time required for the diagnosis of rabies by immunofluorescence from about 5.5 hr to approximately 45 min.


Inhibition of the plaquing efficiency of T4r+ bacteriophage by subtilisin. The mechanism by which the replicative cycle of T4r(+) phage is inhibited by certain nonhost bacterial systems was investigated. Some Bacillaceae, especially Bacillus subtilis, decreased the plaquing efficiency of this virus more than 95% within 24 hr of exposure. Sarcina lutea and Micrococcus sp. both failed to cause any significant change in the infectivity of T4r(+) phage. Preliminary investigations into the nature of the inhibitory substance(s) suggested that an extracellularly elicited protein was at least partially responsible for this effect. Further analysis has implicated subtilisin, an exoprotease from B. subtilis, as the cause of some, if not all, of the observed decrease in plaquing efficiency. Gel-filtration chromatography of control and treated (14)C-labeled T4r(+) phage showed a wide dispersal of phage-specific material of these particles after 24 hr of exposure to pure subtilisin or to expended medium exoprotease from B. subtilis. It was concluded that B. subtilis exoprotease is capable of chemically altering the structure of the phage capsid, thus causing a decrease in its plaquing efficiency.


Serological analysis of morphologically and biochemically similar Streptomyces species. Descriptions of morphological and biochemical characteristics of Streptomyces species have resulted in groups of isolates which appear to be identical or very closely related. Serological analysis has been used to confirm this relationship in some cases and to demonstrate differences in others.


Potentially infectious agents associated with shearling bedpads: effect of laundering with detergent-disinfectant combinations on Staphylococcus aureus and Pseudomonas aeruginosa. Glutaraldehyde-tanned woolskins which are used as bedpads to prevent decubitus ulcers were contaminated with Staphylococcus aureus (ATCC 6538) and Pseudomonas aeruginosa (ATCC 15442). Two methods of exposure, direct contact and aerosol, were used in separate experiments. Attempts were made to decrease the bacterial population placed on the woolskins by laundering them in a quaternary ammonium disinfectant, a phenolic disinfectant, or alkalinized glutaraldehyde, in combination with an anionic or nonionic detergent. The effect of a commercial detergent-sanitizer was also studied. Bacterial populations were significantly reduced in all experiments, but only laundering in glutaraldehyde in combination with either detergent resulted in maximum removal of bacteria. Viable bacteria were usually not detected in the rinse water (<1 viable organism/5 ml of rinse water).


Effect of the purification of virus antigens on the production of specific complement-fixing antibodies. The effect of viral purification procedures on the antibody response of guinea pigs to immunization with reovirus type 2 and echovirus type 19 was investigated. Three grades of antigens were employed: (i) infectious monkey kidney tissue culture fluid (TCF), (ii) virus sedimented in the ultracentrifuge and suspended in phosphate-buffered saline, and (iii) virus purified by centrifugation in CsCl density gradients. The antibody response of the guinea pigs was studied by the hemagglutination inhibition, complement fixation, and serum neutralization tests. Only sera produced from virus purified by CsCl density gradients reacted specifically with homologous antigen in the complement fixation test. Sera from animals receiving tissue culture fluid virus or sedimented virus cross-reacted with heterologous antigens such as tissue culture fluid from uninfected monkey kidney cells. All sera, however, reacted specifically in hemagglutination inhibition and serum neutralization tests. Sera from intranasally infected animals (reovirus type 2), even though reacting specifically in the complement fixation test, had much lower titers than sera from animals inoculated intramuscularly.


Streptomycin uptake by Mycobacterium tuberculosis. Data are presented indicating that (14)C-streptomycin uptake by tubercle bacilli increases as a function of exposure time, is directly and proportionately related to concentration, and involves at least two phases.


Biological activity of the nucleic acids extracted from two aerosolized bacterial viruses. The infectivity of the nucleic acids of piX-174 and MS-2 was examined after aerosolization. It was found that the nucleic acids were undamaged by aerosolization.


Quantitation of residual host protein in chicken embryo-derived vaccines by radial immunodiffusion. Quantitation of soluble residual host protein in chicken embryo-derived vaccines was performed rapidly, economically, and accurately by radial immunodiffusion.


Effect of nitroglycerin at different degrees of contraction of the rabbit aortic strip.


2-Deoxyglucose transportation via passive diffusion and its oxidation, not phosphorylation, to 2-deoxygluconic acid by Pseudomonas aeruginosa.


Alpha-1-antitrypsin: 1. Evidence for two genes causing low concentrations in serum; 2. Association of heterozygosity and chronic obstructive lung disease.


Haemoglobin determination in foetuses and adult mice of two inbred strains and in the F2 generation.


Quantitation of the hepatitis-associated antigen by electroimmunodiffusion.


Studies of the binding of a timothy pollen antigen by sera and serum fractions of atopic individuals using acrylamide gel electrophoresis.


Serologic detection of feline leukemia virus antigens or antibodies.


Neutralization tests for feline leukemia and sarcoma.


Electron microscopic detection of viruses in feline lymphosarcoma.


Naturally occurring upper respiratory infection in cats.


Feline infectious peritonitis: experimental studies.


Toxicity of Pseudomonas aeruginosa bacterins and cell walls to the greater wax moth, Galleria mellonella.


The mechanism of pathogenicity of Pseudomonas aeruginosa. 8. Isolation of hemolymph proteins from Galleria mellonella larvae and their digestibility by the toxic protease.


An assessment of the ability of individual moieties of Pseudomonas aeruginosa endotoxin to induce immunity in larvae of Galleria mellonella.


The private medical practitioner of the 1970s: master or slave of the American health industry.


Evolution of the prokaryotes.

Evolution of the prokaryotes.


Advances in treatment of RH negative blood incompatibility of mothers and infants.


Purification and partial characterization of a proteolytic enzyme from Cephalosporium acremonium (Corda).


Insecticidal activity of microbial metabolites.


Cytoplasmic control of macronuclear events in the cell cycle of Stentor.


The properties of chloroplast ribosomal-RNA.


Demodectic acariasis in a woolly monkey.


An hypothesis to account for a basic distinction between rods and cones.


Speciation in parasitic nematodes.

Speciation in parasitic nematodes.


The action of UK 3883, a novel 2-aminomethyltetrahydroquinoline derivative, against mature schistosomes in rodents and primates.


Genitourinary tuberculosis.

Genitourinary tuberculosis.


Hyperviscosity syndrome.

Hyperviscosity syndrome.


Bone sarcoma: treatment by irradiation, amputation, or a combination of the two. A retrospective survey of all cases of osteosarcoma of the femur and tibia treated at the major centres in the United Kingdom during 1952-9 was carried out in an attempt to assess the respective value of treatment by surgery and radiotherapy and the feasibility of a larger prospective study. Of the 192 patients available for the comparison, those treated by radiotherapy alone did least well. A combination of the two treatments-radiotherapy followed by amputation-produced better results, in terms of survival, than amputation alone; however, the comparatively small numbers involved and the inadequacy of information about the factors dictating the choice of treatment make firm conclusions impossible. A controlled clinical trial on a larger scale might provide answers to the outstanding questions.


Venereal disease in women. II.

Venereal disease in women. II.


Investigation of the mating system of Pseudomonas aeruginosa strain I. VI. Mercury resistance associated with the sex factor (FP).


Microtubules and a contractile ring of microfilaments associated with a cleavage furrow.


The influence of growth-limiting substrate and medium NaCl concentration on the synthesis of magnesium-binding sites in the walls of Bacillus subtilis var. niger.


Effect of glutaraldehyde on protoplasts of Bacillus megaterium.


Taxonomically significant group antigens in Rhizobium.


Surface charges of two types of Bacillus megaterium spores differing in their response to n-butane.


Nucleic acids of animal viruses.

Nucleic acids of animal viruses.


Influence of environment on the content and composition of microbial free amino acid pools.


'Glutamine(amide):2-oxoglutarate amino transferase oxido-reductase (NADP); an enzyme involved in the synthesis of glutamate by some bacteria.


Potentiation by halogen compounds of the lethal action of gamma-radiation on spores of Bacillus cereus.


Mechanism of the inhibition of germination of bacterial spores by gamma-irradiation in the presence of iodoacetamide and iodate.


Mutations in a regulator gene allowing Pseudomonas aeruginosa 8602 to grow on butyramide.


Human tumor immunology.

Human tumor immunology.


The management of plasma cell neoplasms.


Porencephaly, nasofrontal mucoceles, hypertelorism and segmental vitiligo--report of a new neurocutaneous disorder.


Thyrotropin-releasing hormone: biosynthesis by rat hypothalamic fragments in vitro. Biosynthesis of thyrotropin-releasing hormone (L-pyroglutamyl-L-histidyl-L-proline amide) in vitro was studied. Rat hypothalamic fragments were incubated in Krebs-Ringer bicarbonate buffer that contained either (14)C-labeled proline, histidine, or glutamic acid (the three probable precursor amino acids,) and for control purposes each of 16 other naturally occurring amino acids. A number of labeled peptides were synthesized. With the use of synthetic thyrotropin-releasing hormone, detected by the Pauly reagent or with (125)1-labeled thyrotropin-releasing hormone as a marker, thin-layer chromatograms, paper electrophoresis, and carboxymethyl cellulose ion exchange chromatography revealed that only proline, histidine, and glutamic acid were consistently incorporated into peptides associated with the thyrotropin-releasing hormone region. This synthesizing activity was found in stalk median eminence, ventral hypothalamus. and dorsal hypothalamus but not in neural lobe or cerebral cortex. Because the biosynthetic peptide has identical properties with L-pyroglutamyl-L-histidyl-L-proline amide, it is probable that rat thyrotropin-releasing hormone is similar or identical to both bovine and porcine thyrotropin-releasing hormone and that the native material is present in the pyroglutamyl form in tissues.


Parenteral hyperalimentation. A useful surgical adjunct.


Dynamic visual acuity as an index of eye movement control.


Summary of recent abstracts. I. Cholera.


Chromosomal aberrations and their relation to malignancy in meningomas: a meningoma with ring chromosomes.


Acute adverse reactions to LSD in clinical and experimental use in the United Kingdom.


Central autonomic effects of prostaglandin F2 alpha on the cardiovascular system of the dog. 1. Prostaglandin F(2alpha) infused into the vertebral artery of the anaesthetized greyhound in doses which had no effect when given intravenously ((8-64 ng/kg)/min) caused an increase in blood pressure and heart rate.2. This response was not significantly altered by beta-adrenoceptor blockade with propranolol (10 mg i.v.) or by cervical cord section at C(4-6).3. The tachycardia was abolished and the pressor response greatly reduced by vagotomy or atropine (250 mug/kg i.v.).4. The pressor response which remained after vagotomy was abolished by subsequent sympathetic blockade with bethanidine (2-3 mg/kg i.v.) or bretylium (10 mg/kg i.v.).5. In contrast to the effects of propranolol or cervical cord section bethanidine (4-5 mg/kg i.v.) or bretylium (10 mg/kg i.v.) significantly reduced blood pressure and heart rate responses to intravertebral prostaglandin F(2alpha). This result suggests that bethanidine and bretylium have some central actions.6. It is concluded that the cardiovascular effects of intravertebral infusions of prostaglandin F(2alpha) are mediated by the autonomic nervous system and that the preferential pathway is withdrawal of vagal tone to the heart.


Localization of intrinsic factor and complement fixing intrinsic factor--intrinsic factor antibody complex in parietal cell of man.


The intravenous administration of equine antilymphocytic globulin in renal transplant recipients and the detection of circulating antibodies to equine globulin.


Characterization of precipitating antibodies to E. coli O antigen in infants and children with acute pyelonephritis.


Total counts of Bacillus megaterium and Clostridium butyricum spore suspensions using a microlitre syringe.


Structural characterization of a human monoclonal IgA protein.


A method for the detection of gelatinase production by bacteria.


Ultrastructure of spore coats produced by lethal germination of spores of Bacillus megaterium 9885.


Purification of Pseudomonas aeruginosa endotoxin by gel filtration on Sepharose 4B.


Sequence and structure homologies in bacterial and mammalian-type cytochromes.


Ribosomal RNA synthesis is turned off during sporulation of Bacillus subtilis.


Progress in neurosurgical treatment of intractable pain from Simpson's day.


Epidemiology of Pseudomonas aeruginosa infection in hospitals. 1. Pyocine typing of Ps. aeruginosa.


Acid-base effects of topical mafenide acetate in the burned patient.


Abetalipoproteinemia in acrodermatitis enteropathica.


Similarities in protozoan and flatworm habituation behavior.


Gastrointestinal bleeding as a manifestation of von Recklinghausen's disease of the skin.


Histopathological study of endochondral ossification of femur in infants. Histological observation on 193 autopsy cases.


Rosette formation by sensitized human erythrocytes on various heterologous macrophages.


Immunofluorescent studies of basic nuclear proteins.


Rosette formation by peripheral lymphocytes. II. Inhibition of the phenomenon.


Radiosensitization with iodine compounds. 3. Macromolecular synthesis and repair in Bacillus subtilis irradiated in the presence of iodoacetic acid, potassium iodide or potassium iodate.


Epidemiology of Pseudomonas aeruginosa infection in hospitals. 2. "Fingerprinting" of Ps. aeruginosa strains in a study of cross-infection in a children's hospital.


Attraction of triatomine bug vectors of Chagas's disease to betalights.


Detection of Australia-SH-antigen in urine.


Peer review in biliary tract surgery.

Peer review in biliary tract surgery.


Typhoid and paratyphoid immunization.

Typhoid and paratyphoid immunization.


Cannulation of the umbilical vein.

Cannulation of the umbilical vein.


Some aspects of adult megacolon.

Some aspects of adult megacolon.


Telencephalic remnants in simian and human anencephaly.


Synthesis of thyroglobulin peptides by pig thyroid polyribosomes.


Time course of serum estrogen and luteinizing hormone (LH) concentrations during the menstrual cycle of the rhesus monkey.


The seasonal variation in the concentration of protozoa and Oscillospira guilliermondii organisms in the rumen fluid of grazing buffalo.


The effect of anesthesia and surgery in pregnant rhesus monkeys.


Control of responding by location of auditory stimuli: rapid acquisition in monkey and rat. Monkeys require a considerably larger number of trials to bring responding under the control of the location of an auditory stimulus than cats, rats, and bats with the same experimental procedures. The present experiment sought to determine the conditions necessary for rapid acquisition of control of responding by location of noise and tone bursts in the monkey. Monkeys were run in an enclosure that contained four loudspeakers and four manipulanda. Two conditions were used in training. In the adjacent condition, a stimulus (noise or tone burst) was presented through one or other of two speakers and a response on the manipulandum adjacent to the speaker was reinforced with food. In the nonadjacent condition, a stimulus was presented through one of two speakers and a response on a manipulandum remote from the speaker was reinforced with food. Acquisition of control was measured by change in the percentage of reinforced responses during training. In the adjacent condition, responding came under control of location within zero to three sessions. In nonadjacent conditions, the animals required 14 to 20 sessions to come under control of location. These latter numbers are comparable to those reported in the literature for localization discrimination in monkeys.


Instrumental chemolysis of renal calculi: indications and dangers.


Morphological changes in Pseudomonas aeruginosa treated with rod-shaped pyocin 28.


Evidence for the presence of inhibitor and some trials for its prevention in radioimmunoassay of plasma glucagon by two-antibody system.


Influence of propionylpromazine on liver-function tests and hepatic structure in cattle.


Allogenic heterotopic canine liver homotransplantation. II. Pathological observations.


Changes in lactate dehydrogenase isoenzymes of liver, heart and skeletal muscles of the pig.


The heart of the duck.

The heart of the duck.


The in vitro activity of several antibiotics against Pseudomonas of bovine udder origin.


Further studies on feline respiratory virus diseases. 2. Immunodiffusion tests.


Stimulation of liver catalase synthesis in rats by ethyl-alpha-p-chlorophenoxyisobutyrate.


Studies on the status of tyrosyl residues in cobrotoxin.


Amino acid composition and peptide maps of Bacillus cereus 569-H penicillinase.


The action of crude bacterial alpha-amylase on tropocollagen. A case of strictly limited proteolysis.


Isoenzymes of carbonic anhydrase I from primate red blood cells.


A monospecific antibody to human sulfatase A. Preparation, characterization and significance.


Interaction of lactoferrin with agar gels and with Trypan Blue.


The role of human chorionic somatomammotropin (HCS) and gonadal steroids in gestational diabetes.


Radiation duodenitis.

Radiation duodenitis.


Nodal spread in relation to penetration and differentiation.


The role of sorbent in the absorbed fluorescent treponemal antibody (FTA-ABS) test.


The "unique" metal-binding properties of metalloenzymes.


Incidence of the Australia antigen in various diseases.


Pituitary gonadotrophic activity of thyroidectomized prepuberal female rhesus monkeys.


Morphology and development of the macronuclei of the ciliates Stylonychia mytilus and Euplotes aediculatus.


Properties of rat homocytotropic antibody and inhibition of PCA reaction with rat homocytotropic antibody by Cu-chlorophyllin.


Unusual albumin agglutinating property in the serum of a Japanese female patient with rectal cancer.


X-chromosome linked inheritance of elevated thyroxine-binding globulin in association with goiter.


Psychobiological studies of aggressive behaviour.


Absence of enhancing antibody in cell mediated immunity to tumour heterografts in protein deficient rats.


Plasma testosterone, dominance rank and aggressive behaviour in male rhesus monkeys.


Conformation radiotherapy for nasopharyngeal tumor.


The effect of intravascular and extravascular coagulation on serum fibrin degradation products in rats.


Gel diffusion and immunoelectrophoretic analysis of some Venezuelan Trypanosoma cruzi strains.


Prothrombin level and activity in the abnormal factor X (factor X Friuli) hemorrhagic disorder.


Immuno-diffusion reactions among various samples of Leishmania.


Syndrome of hydrocephalus caused by saccular aneurysm of the basilar artery.


Osteochondritis dissecans and anomalous centres of ossification: a review of 80 lesions in 61 patients.


Congenital pseudarthrosis of the tibia.

Congenital pseudarthrosis of the tibia.


Malignant transformation of cells cultivated in vitro. A model of carcinogenesis with special reference to "spontaneous" malignant transformation.


The nitroglycerine ointment test--a double-blind examination.


Studies on the anti-plasma cell serum. I. Selective suppression of gamma-2 hemolysin in mice.


Immune responses of normal infants to cow milk. I. Antibody type and kinetics of production.


Suppression of spontaneous autoimmune thyroiditis in obese strain (OS) chickens with anti-bursa and anti-thymus sera.


In vitro studies of chicken lymphoid cells. II. The anamnestic response of spleen cells from control-irradiated and bursectomized-irradiated chickens to sheep erythrocytes.


The ovarian vein and hydronephrosis of pregnancy. Experimental studies in the rhesus monkey (Macaca mulatta).


"Steroid-induced" mydriasis and ptosis.

"Steroid-induced" mydriasis and ptosis.


Antibacterial activity of diaveridine, trimethoprim, and selected sulfonamides in prostatic fluid.


Congenital defects of the tibia in siblings with neurofibromatosis.


Pulmonary complications of esophageal abnormalities.


Urological aspects of chylous ascites. Case report.


Effects of synthetic pyroglutamyl-histidyl-proline-amide on serum levels of thyrotrophin, cortisol, growth hormone, insulin and PBI in normal subjects and patients with pituitary and thyroid disorders.


A comparative study of the adrenergic nerves to the anterior eye segment of some primates.


Viral disease of goslings. 3. Isolation, properties and antigenic pattern of the virus strains.


Enterococcus occurrence in organs and muscles of slaughtery swine.


Microtoposcopic investigation of the peristriate cortex of the squirrel monkey.


35Cl nuclear magnetic resonance studies of metal binding to bovine serum albumin.


Monitoring under anaesthesia, with reference to subarachnoid haemorrhage, and the T-wave as an electrocardiographic manifestation.


Properties and localisation of mycobacillin-synthesising enzyme system in Bacillus subtilis B3.


Heparin treatment of intravascular coagulation accompanying hemolytic transfusion reactions.


The regulation of the beta-methylgalactoside transport system and of the galactose binding protein of Escherichia coli K12.


The immunochemistry of peptidoglycan. Separation and characterization of antibodies to the glycan and to the peptide subunit.


C-phycocyanin monomer molecular weight.

C-phycocyanin monomer molecular weight.


Structure of phycocyanobilin.

Structure of phycocyanobilin.


The anesthetic properties of 1, 1,-difluoro-2, 2, 2-trichloroethyl methyl ether.


Lateral plication of synthetic sack for large gastroschisis and omphalocele defects.


Gonorrhea in women.

Gonorrhea in women.


Transfer of gonococcal urethritis from man to chimpanzee. An animal model for gonorrhea.


Neurologic aspects of malignant external otitis: report of three cases.


Immunological studies for the assay of human chorionic gonadotrophin. 1. Fundamental studies of the immunological method for the assay of human chorionic gonadotrophin.


Gram negative bacilli infection in the surgical field. Second report.


Modification of dose distribution in high-energy electron beam treatment.


Immature joint cartilage and the homograft reaction.


The treatment of Dupuytren's fracture-dislocation of the ankle.


Functional anatomy of the cardiac nerves in the baboon.


Ultrastructure of middle ear mucosa and organization of ciliary matrix.


The pathology of acute and chronic infections of the middle ear cleft.


Tympanosclerosis.

Tympanosclerosis.


Gelfoam as an implant in oval window following stapedectomy.


A round trip journey from anesthesia to psychiatry via the fluorinated ethers.


Importance of the design of an exercise protocol in the evaluation of patients with angina pectoris.


Clinical and circulatory effects of isosorbide dinitrate. Comparison with nitroglycerin.


Radioisotope studies in isolated perfused baboon kidneys.


Erythropoietin release from preserved kidneys.


Purification and properties of phosphoacetylglucosamine mutase.


Timing of mucopeptide and phospholipid synthesis in sporulating Bacillus megaterium.


Cromolyn treatment of asthma. Trials in corticosteroid-dependent asthmatics.


Hyperosmolar nonketotic coma with hyperglycemia: abnormalities of lipid and carbohydrate metabolism.


Hyperalimentation.

Hyperalimentation.


Evaluation of current diagnosis and treatment of primary malignant tumors of the bone.


Binding of lipid by soluble and membrane proteins isolated from rat liver.


Therapeutic keratoplasty in Pseudomonas pyocyaneus corneal ulcers.


The radioisotope renogram in carcinoma of the cervix.


Prostaglandins and habitual abortion.

Prostaglandins and habitual abortion.


Production of active phage from B. subtilis cultures.


Quantity, quality, and responsibility in aging research.


National goals and priorities in the social welfare of the aging.


A comparison of various measures of sensitivity of M. tuberculosis to pyrazinamide.


Chemical fractionation of Brucella SPP.

Chemical fractionation of Brucella SPP.


Comparative effects of rapid thawing, low molecular dextran and sympathectomy in cold injury in the monkeys.


Evaluation of the effect of Rifampin on the nasopharyngeal carriage of Neisseria meningitidis. A preliminary report.


Joint action of beta-lactam antibiotics.


Some diseases involving the eye and the skin.


Determination of in vivo persistence of tantalum dust following bronchography using reactor-activated tantalum and total body counting.


The biochemical basis of the skeletal changes in chronic uremia.


Shunting via a diverticulum of the membranous portion of the ventricular septum.


A statistical study of the intrauterine selection factors related to the ABO system. II. The analysis of foetal mortality data.


Intracavitary chemotherapy for multiple non-invasive bladder tumours.


Some biochemical and kinetic properties of sheep uterine endometrial alkaline phosphatase.


Intravesical thiotepa in the treatment of transitional cell bladder carcinoma.


Choice of penicillins for gonorrhoea.

Choice of penicillins for gonorrhoea.


Inhibition studies of cathepsins A, B, C and D from rat liver lysosomes.


Clomacran and chlorpromazine in psychotic outpatients: a controlled study.


Double blind clinical study comparing doxepin and imipramine in depression.


Clinical trial of imidazoline (DH-524) as an anti-depressant.


CL-67,772: a preliminary evaluation of a potential antidepressant compound. Animal and human correlations.


Behavior problems in nursing home patients: treatment with thioridazine.


Diphenylhydantoin sodium (Dilantin) withdrawal in non-epileptics.


Bronchodilator action of drug combinations in asthmatic patients. Ephedrine, theophylline and tranquilizing drugs.


Treatment of acute and chronic constipation with bisoxatin acetate and bisacodyl. Double-blind crossover study.


A double-blind comparison of alclofenac and indomethacin in osteoarthritis of the hip.


Laboratory and pharmacologic studies of BL-P-1322 (cephapirin sodium) in children.


Hydrolysis. A requisite for morphine detection in urine.


Petit mal status. A variety of non-convulsive status epilepticus.


Animals as a source of toxoplasma infections in man.


Demonstration of subclinical infections in the course of an epidemic of infective jaundice with positive Australia (SH) antigen.


Certain aspects of SPO1 development.

Certain aspects of SPO1 development.


RNA synthesis during bacteriophage SPO1 development: six classes of SPO1 RNA.


Transcription during bacteriophage SPO1 development: mutations affecting the program of viral transcription.


Isolation and biological activity of mammalian helical polyribosomes.


Heterogeneity in nucleotide composition of Bacillus subtilis DNA.


The development of cerebral collateral circulation following occlusion of vessels in the neck. An experimental study in baboons.


Convulsive effects of bicuculline in photosensitive baboons (Papio papio) and rhesus monkeys (Macaca mulatta).


Deciduomal reaction induced in rhesus monkeys by tubal injection of arachis oil and by the presence of an intrauterine device.


The isoenzymes of carbonic anhydrase: tissue, subcellular distribution and functional significance, with particular reference to the intestinal tract. 1. The total carbonic anhydrase activity in some guinea-pig tissues has been measured using a pH-stat procedure. Stomach, gall bladder, proximal colon and caecum all possess more carbonic anhydrase activity per unit amount of protein than does whole blood.2. The carbonic anhydrase activity of the small intestine is low. Reasons are given for supposing that activity found there is not entirely due to contamination by whole blood, and it is suggested that in this tissue the enzyme may be localized in some cell type other than the columnar absorbing cells.3. Evidence is presented which indicates that heavy metals interfere with the activity of the enzyme as measured in tissue homogenates.4. The distribution and concentration of the two major isoenzymes of carbonic anhydrase have been measured in different tissues. Blood and proximal colon contain both isoenzymes in comparable concentrations, the ratio of the concentration of the ;low activity' isoenzyme to that of the ;high activity' being about 2. The gastric mucosa contains much ;high activity' carbonic anhydrase, but only a negligible amount of the ;low activity' isoenzyme. In the caecal mucosa, the ;low activity' isoenzyme is predominant, the ratio of its concentration to that of the ;high activity' isoenzyme being about 9. It is also found that more than 1.5% of the protein in the caecal mucosa is accounted for as carbonic anhydrase enzymes.5. It is found that some 45% of the total carbonic anhydrase activity of sucrose homogenates of the guinea-pig colon is bound to particles. The activity is located mainly in the nuclear and microvillous fraction and in the ;high-speed supernatant' fraction. The form of enzyme bound is largely of the ;high activity' variety. When the tissue is homogenized in potassium chloride solutions less than 4% of the total activity is recovered in particulate fractions. The amount of activity which is bound to particulate fractions increases as the ionic strength or pH of the homogenate is lowered.6. The findings are discussed in relation to the possible physiological roles of the isoenzymes in tissues other than blood. Possible relationships between the presence of the enzymes and the metabolism and transport of ammonium and fatty acids are considered.


Structural similarities between alpha-lytic protease of Myxobacter 495 and elastase.


Effect of isozanthopterin on the activity of transforming DNA in Bacillus subtilis.


Calibration of a whole-body counter suitable for use in routine clinical investigations.


Middle lobe syndrome.

Middle lobe syndrome.


Effect of service period on the milk production and lactation length of buffaloes in Italy.


Studies on the effect of Ambilhar on iron metabolism.


Service period, calving interval and dry period in Italian buffaloes.


The fate of Mycobacterium tuberculosis within macrophages of guinea pigs.


A case of myelogenous leukemia in the cat.


Blood coagulation defects in calves fed on toxic groundnut (aflatoxin).


Bacillus cereus pneumonia and bacteremia. A case report.


Ectopic spleen in an Rh incompatible infant.


Serologic typing of Nocardia asteroides by immunodiffusion.


Primary drug resistance: a continuing survey of tubercle bacilli in a veteran population within the United States. 8. The influence of culture medium on the observed frequency of resistance.


On the formation of an intermediate in the adenine phosphoribosyltransferase reaction.


Plasma histaminase levels in status asthmaticus.


The effects of exposure to chewing tobacco on the oral mucosa of monkey and man. A histological and ultrastructural study.


Porphyrin and heme biosynthesis from Endogenous and exogenous delta-aminolevulinic acid in Escherichia coli, Pseudomonas aeruginosa, and Achromobacter metalcaligenes.


The orthopaedic management of brain-injured children.


Bipartite atlas. Review of the literature and report of a case.


Subluxation of the ankle without fracture of the fibula. A case report.


Experimental traumatic paraplegia. The vascular and pathological changes seen in reversible and irreversible spinal-cord lesions.


Pemphigus antibodies: their role in disease.


Lysosomes in mouse melanoma.

Lysosomes in mouse melanoma.


Study on a new antidiabetic agent, glibenclamide (HB419). A clinical evaluation and comparative study.


The pathology of tumors. 1. Introduction, precancerous lesions, benign lesions that resemble cancer.


Lipoproteins in cancer patients.

Lipoproteins in cancer patients.


Von Recklinghausen's disease.

Von Recklinghausen's disease.


Experience with combined screening for the detection of carcinoma of the cervix and carcinoma of the breast.


Treatment of toxicity in cancer chemotherapy.


The cancer information service: a shared responsibility.


Absence of histone in the blue-green alga Anabaena cylindrica.


Who? Me? I haven't got time.

Who? Me? I haven't got time.


Cancer information services in the United States.


Animal models of immediate-type respiratory allergy.


Unproven methods of cancer management: M-P virus.


Primate management in Asia.

Primate management in Asia.


Ecology of anthrax.

Ecology of anthrax.


Monosodium glutamate: absence of hypothalamic lesions after ingestion by newborn primates. After receiving monosodium glutamate by stomach tube, the brains of infant macaques were perfused for examination by light and electron microscopy. No morphological differences were observed in the hypothalamic regions of treated and control monkeys. However, inadequately fixed tissue had the same appearance as that of the previously reported brain lesion in a newborn monkey.


Long-term intubation of the biliary tract with silastic catheters.


The synthesis of antibiotic sugars.

The synthesis of antibiotic sugars.


Studies of common baldness of the stump-tailed macaque (Macaca speciosa). VI. The effect of testosterone on common baldness.


Osteoma cutis.

Osteoma cutis.


Studies on lytic activities of Chondrococcus coralloides (Myxobacteriales). I. Purification and some properties of the bacteriolytic and proteolytic activity.


Multinucleate giant cells in livers of marmosets given aflatoxin B1.


Complement-fixing antibodies in cattle experimentally infected with Theileria annulata or vaccinated with tissue cultures.


Comparative metabolism of radionuclides in mammals: a review.


Influence of incidence angle of monoenergetic neutrons on dose equivalent distribution for recoil nuclei in man's phantom.


Immunofluorescence studies in Waldenström's macroglobulinemia.


Summary of recent abstracts. IV. Trypanosomiasis.


Causative fungus agent of leucoencephalomalacia in equine animals.


Immunoprophylaxis of Pseudomonas aeruginosa.


Chronic active hepatitis. Report of two sisters with positive lupus erythematosus preparations.


Disseminated gonococcal infection.

Disseminated gonococcal infection.


Gross conformation of free polypeptide chains from rabbit immunoglobulin G. I. Heavy chain.


Gross conformation of free polypeptide chains from rabbit immunoglobulin G. II. Light chain.


Recovery of native conformation of rabbit immunoglobulin G upon recombination of separately renatured heavy and light chains at near-neutral pH.


Characterization and chemical modifications of toxins isolated from the venoms of the sea snake, Laticauda semifasciata, from Philippines.


Purification and properties of an alkaline phosphatase of Bacillus licheniformis.


Molecular weight and subunits of the alkaline phosphatase of Bacillus licheniformis.


Coprecipitation of carbonic anhydrase by 1,1-bis(p-chlorophenyl)-2,2,2-trichloroethane, 1,1-bis(p-chlorophenyl)-2,2-dichloroethylene, and dieldrin.


Unfolding of the 30S ribosomal subunit of Escherichia coli and the conformation of the unfolded subunit. Parallel sedimentation and optical rotatory dispersion studies.


The current status of gonorrhoea control.


Comparative studies on the properties of thyroglobulins from various animal species.


Purification of human placental lactogen.


Effect of synthetic thyrotropin-releasing factor (TRF) on pituitary TSH secretion in man.


Therapeutic effects of nurse-patient relationships.


Effect of exercise on mineral and organic bone turnover in swine.


A computer evaluation of the demand for short-term or long-term care for mentally subnormal children.


Measurement of the distribution of cardiac output in unanesthetized rats.


Ionic aluminum (3) in generator eluate as an erythrocyte-agglutinating agent.


95Zr and 124Sb in 99Mo-99mTc generators.


Improvement in labeling erythrocytes with 99mTc-pertechnetate.