The control by internal calcium of membrane permeability to sodium and potassium. 1. A study has been made of the relationship between the concentration of internal calcium and the permeability of human red cell membranes to sodium and potassium.2. Fresh red cells contain very little calcium, but after being depleted of ATP by ageing they took up calcium from Ringer solution. The entry was unaffected by external sodium and potassium but was markedly pH dependent. When supplied with energy, calcium-loaded cells actively extruded calcium by a saturable process which was also unaffected by the distribution of sodium and potassium across the membranes. The activity of the calcium pump was sufficient to maintain the low internal concentration found under physiological conditions.3. Raising internal calcium in metabolically poor cells caused a loss of cell potassium which was greater than the concomitant sodium gain. These changes were reversed when ATP was supplied. External calcium had no effect. The increase in permeability to sodium and potassium was enhanced by the simultaneous addition of fluoride, and, even more so, of iodoacetate. These inhibitors had no effect on membrane permeability unless calcium was also present. Inosine potentiated the action of fluoride and iodoacetate in causing potassium loss, by allowing more calcium to enter the cells.4. The results suggest that the permeability of human red cell membranes to sodium and potassium is regulated by internal calcium, which in turn is controlled by a calcium pump that utilizes ATP.


The role of cord factor in pathogenesis of tuberculosis.


Phage genetic analysis of the mechanism of synthesis of diphtherial toxin in nontoxinogenic Corynebacterium diphtheriae directed by nonlysogenizing phage.


The influence of the chloride gradient across red cell membranes on sodium and potassium movements. 1. A study has been made to see whether active and passive movements of sodium and potassium in human red blood cells are influenced by changing the chloride gradient and hence the potential difference across the cell membrane.2. Chloride distribution was measured between red cells and isotonic solutions with a range of concentrations of chloride and non-penetrating anions (EDTA, citrate, gluconate). The cell chloride concentration was greater than that outside with low external chloride, suggesting that the sign of the membrane potential was reversed. The chloride ratio (internal/external) was approximately equal to the inverse of the hydrogen ion ratio at normal and low external chloride, and inversely proportional to external pH. These results show that chloride is passively distributed, making it valid to calculate the membrane potential from the chloride ratio.3. Ouabain-sensitive (pump) potassium influx and sodium efflux were decreased by not more than 20 and 40% respectively on reversing the chloride gradient, corresponding to a change in membrane potential from -9 to +30 mV. In contrast, passive (ouabain-insensitive) movements were reversibly altered - potassium influx was decreased about 60% and potassium efflux was increased some tenfold. Sodium influx was unaffected by the nature of the anion and depended only on the external sodium concentration, whereas ouabain-insensitive sodium efflux was increased about threefold. When external sodium was replaced by potassium there was a decrease in ouabain-insensitive sodium efflux with normal chloride, but an increase in low-chloride medium.4. Net movements of sodium and potassium were roughly in accord with the unidirectional fluxes.5. The results suggest that reversing the chloride gradient and, therefore, the sign of the membrane potential, had little effect on the sodium pump, but caused a marked increase in passive outward movements of both sodium and potassium ions.


Studies on antitumor activity of bacterial lipopolysaccharide.


Characteristics of CIR (cell injuring reaction) active substance in culture fluid of Bacillus cereus, "MS-1063 strain".


Pharmacology of a new anti-parkinsonian drug: KAO-264.


The energetic economy of the organism in animal evolution.


The relation of biology to physics and chemistry--an evaluation of some recent issues in the philosophy of science.


Proposal of general, ethical statement for natural scientists.


A contribution to the problem of the concept "biological clock". I.


The histochemistry of the cytoplasm of the developing mammalian egg.


Leukocyte alkaline phosphatase in iron deficiency.


Studies on erythropoiesis inhibiting factor (EIF). II. The site of formation of EIF.


On the influence of normal and leukemic human leukocytes on blood coagulation.


One- and two-stage factor VIII-activity in acute leukaemia.


Partial and severe peroxidase and phospholipid deficiency in eosinophils. Cytochemical and genetic considerations.


The distribution of erythropoietic bone marrow in the mouse.


Deficiency and absorption of iron in man.


Gonococcal vulvovaginitis and possible peritonitis in prepubertal girls.


Analysis of blue-green and red algal ribosomal-RNAs by gel electrophoresis.


Nitrogen fixation by unicellular blue-green algae.


Incidence of congenital defects in South Wales, 1964-6.


Excretion of porphyrins by bacteria.

Excretion of porphyrins by bacteria.


Characterization of induced homocytotropic antibody to ragweed and timothy pollen in non-atopic dogs.


Detection of gamma-crystallins in the developing amphibian lens by peroxidase-labelled antibodies.


Caffeine removal from growth media by microorganisms.


Cytological studies on exfoliated cells in serous effusions.


Supervoltage radiation response in epidermoid carcinoma of the oesophagus.


Serial specific neutralization of reagins of cross-reactive human serum in monkeys.


Making the gradient?

Making the gradient?


Carbonic anhydrase and the regulation of photosynthesis.


Biological components of human reproduction. Studies of their variations in population groups.


Bilateral neurofibroma of the intrathoracic vagus associated with von Recklinghausen's disease.


An unusual association of multiple meningiomas, intracranial aneurysm, and cerebrovascular atherosclerosis in two young women.


Hormonal influences upon ethylene glycol poisoning.


Artificial sweeteners as urinary bladder carcinogens.


The molecular aggregation of anthranilate synthase in Bacillus subtilis.


Rat intrinsic factor. Partial purification and characterization.


Immunological and chemical comparison on heterogeneous basic glycoproteins in human parotid saliva.


Enhancement of Bacillus cereus spore lytic enzyme by a heat-labile non-dialyzable factor in spore extracts.


Estriol modification of the effects of progesterone and human chorionic somatomammotropin on glucose tolerance and plasma insulin in rhesus monkeys.


Cell walls of a teichoic acid deficient mutant of Bacillus subtilis.


A study of the single radial hemolysis in gel system. I. Factors affecting the model.


A study of the single radial hemolysis in gel system. II. Application of the model to mouse 19S hemolytic antibodies.


Dissociation of Waldenström's immunoglobulin M by the disulfide interchange reaction.


Immunochemistry of an insect protease, cocoonase, and its zymogen.


Polymer-induced precipitation of antigen-antibody complexes: "precipiplex" reactions.


Root resorption after restorative procedures.


Binding of erythromycin to Escherichia coli ribosomes.


Locomotion and activity phasing of some medium-sized mammals.


Fusion in vitro of membranes from animal cells.


Effect of delayed hyperventilation on experimental cerebral infarction.


Activation of spike and wave discharge in patients with generalized seizures.


Simultaneous determination of renal blood flow and glomerular filtration rate without ureteric catheterization.


Factors affecting the differential dissolution of human enamel in acid and EDTA. A scanning electron microscope study.


Wound healing in monkey (Macaca irus) mandible: effect of elevating periosteum on formation of subperiosteal callus.


The neutral lipids of bovine bone.

The neutral lipids of bovine bone.


Trends in the treatment of pelvic malignant diseases.


Total body irradiation for myelomatosis.


Ovarian ablation for breast cancer.

Ovarian ablation for breast cancer.


Unusual cases of myelomatosis.

Unusual cases of myelomatosis.


Lymphosarcoma of the lung with monoclonal (IgM) gammopathy. A clinicopathologic, histochemical, immunologic, and ultrastructural study.


Susceptibility of Erythrocebus patas monkeys to rubella virus.


Isolation of mycoplasms and other organisms from the placenta after caesarean section.


The non-antigenicity of intact ova of Clonorchis sinensis.


Studies on the toxicity of Sarcocystis.

Studies on the toxicity of Sarcocystis.


Nalidixic acid (negram) disc technique for the rapid isolation of faecal streptococci from mixed flora.


Identity and glycogen-fermenting ability of lactobacilli isolated from the vagina of pregnant women.


Experimental botryomycosis produced by Pseudomonas aeruginosa.


Relapse of urinary tract infection in the presence of urinary tract calculi: the role of bacteria within the calculi.


Appearance of a brain-specific antigen (S-100 protein) in the developing rat brain.


The regional distribution, age dependent variation and species differences of brain arylsulphatases.


Brain ganglioside patterns of vertebrates.


Newer hypolipidemic agents.

Newer hypolipidemic agents.


Cord IgM levels in Latin American neonates.


A hard but heartening year for HAS.

A hard but heartening year for HAS.


A comparison of methods for the determination of iron absorption using a whole-body counter.


Measurement of total body potassium with a shadow shield whole-body counter: calibration and errors.


Maternal-fetal endocrine interrelations: demonstration of TSH release from the fetal hypophysis in pregnant rats administered synthetic TRH.


New antigens in lactose.

New antigens in lactose.


Laboratory studies of epidemic strains of Echovirus 9.


Estrogenic properties of quinestrol and estradiol-17 beta in the ovariectomized rhesus monkey.


The cytological localization of erythropoietin in the human kidney using the fluorescent antibody technique.


The metabolism of estradiol-17 beta-4-14C in the newborn rhesus monkey.


Prostate antigens and antibodies.

Prostate antigens and antibodies.


Immunogenetic studies of rhesus monkeys. I. The G blood group system.


Insulin and glucagon in the regulation of removal rate of exogenous lipids from the blood in dogs.


A double-blind, double cross-over trial of prenylamine in angina pectoris.


Initial temperature sensitivity of streptomycin-dependent and tryptophan transformants of Bacillus subtilis.


Reappraisal of the toxicology of ethylene glycol. II. Metabolism studies in laboratory animals.


Metal carcinogenesis in experimental animals.


Reappraisal of the toxicology of ethylene glycol. 3. Mitochondrial effects.


The serological response of Mongolian domestic animals to rickettsia prowazeki and mooseri antigens.


The microbiologic evaluation and enumeration of postmortem specimens from human remains.


Isolation of acid-fast bacilli from contaminated specimens.


Tuberculosis outbreak in an Alabama High School.


The brave die sooner.

The brave die sooner.


Blood groups and the rhesus factor.

Blood groups and the rhesus factor.


Effects of brief separation from mother on rhesus monkeys. To summarize, data on the course of development of mother-infant relations in rhesus monkeys have been presented; a method for teasing apart the relative roles of mother and infant in causing changes or differences in the interaction described; and the complexity of the social nexus, within which the relationship is set, stressed. When the mother is removed for a few days, the infant calls a great deal at first and then shows depressed locomotor and play activity. These symptoms may last for a month after the mother's return. Simple tests given 6 months and even 2 years later strongly suggest that the differences (between infants that have had such a separation experience and infants that have not) are persistent. Are these data relevant to the human case? The rhesus monkey has no verbal language and a much less complex social development than man. Furthermore, its social environment is quite different from that found in any human culture. Parallels between monkey and man must therefore be scrutinized carefully before being used as a basis for generalization. But the facts show that a brief separation experience produces in rhesus monkey infants symptoms that are very similar (except for the apparent absence of a "phase of detachment" on reunion) to those in human infants (25). While age of separation, within the rather narrow limits used here, was a variable of minor importance, the effects of the separation varied, as in the human case, with the length of the separation experience and the sex of the infant. Differences in the techniques of experimenters, as well as differences in the species, prevent precise comparisons of the roles of the mother-infant relationships; nevertheless, the nature of the relationship appears to be an important variable in both monkey and man. There would seem, therefore, to be strong reasons for thinking that we are dealing with comparable phenomena. If that is the case, the fact that monkeys function at a simpler conceptual level than man limits the complexity of the explanatory hypotheses necessary in the human case. In addition, the finding that such a brief separation experience, involving removal of the mother but no exposure to a strange environment, can produce effects lasting for months or years in rhesus monkeys strengthens the evidence that long-term effects may occur also in man. Finally, this analysis provides bases for attempts to predict individual differences in the effects of a period of separation on rhesus infants, and the parallels with man suggest that examination of the same variables in the human case would be worthwhile.


Transcription of nonrepeated DNA in mouse brain. Under normal conditions of DNA renaturation, about 60 percent of mouse DNA fragments renature at a rate consistent with their being present only once per sperm. These nonrepeated sequences (also called single-copy or unique) may be used in RNA-DNA hybridization experiments to provide quantitative estimates of RNA diversity. About 10 percent of the mouse single-copy sequences are transcribed in mouse brain tissue. Estimates of about 3 percent were obtained for mouse liver and kidney RNA's. If only one of the complementary DNA strands is transcribed, this hybridization value implies that the equivalent of at least 300,000 different sequences of 1000 nucleotides are expressed in mouse brain tissue. It is suggested that the large amount of DNA in mammals is functionally important, and that a substantial proportion of the genome is expressed in the brain.


Comparative sensitivity of complement fixation, counterimmunoelectrophoresis, radial, and double diffusion for detection of Australia antigen.


Autoradiographic studies of RNA metabolism in human leukaemic blast cells.


Clinical experience with anticoagulant therapy in the management of disseminated intravascular coagulation in children.


Ultrastructural aspects of bone marrow and peripheral blood cells in a case of plasma cell leukemia.


The early stages of glass-contact haemolysis.


Gu, a variant of G.

Gu, a variant of G.


Carrier proteins of folic acid activity in human serum.


The quantitative bone marrow and spleen composition in male NMRI and CBA mice.


Detection of fetal maternal hemorrhage: an evaluation of serological tests related to Rh-o(D) immune globulin (human).


The occurrence of homozygous hemophilia in the female.


Prophylaxis of joint hemorrhages in hemophilia.


Proliferation disturbances of erythroblasts in congenital dyserythropoietic anemia type I and II.


Decreased hemoglobin A2 concentration in iron deficiency anemia.


Lymphoid myelofibrosis. About 10 further observations.


RNA and protein synthesis in proliferating and non-proliferating blast cells of human acute leukaemia.


Significance of abnormal LDH isoenzyme pattern in human platelets.


Estimation of the probability of paternity. I. Blood groups of the ABO and MN systems, D-antigen of the Rh system.


Transfer of radioactive iodopyracet (131-I-Diodrast) from the fetus into amniotic fluid after tracheal ligation.


Posterior choanal atresia.

Posterior choanal atresia.


Colistin-sensitive Proteus organisms: including indole-negative Proteus vulgaris, non-swarming on first isolation.


Bile acid involvement in azo dye carcinogenesis.


Altered transfer RNA methylase patterns in Marek's disease tumors.


Modified bases and transfer RNA function.


Microbial content in non-sterile pharmaceuticals. VII. Topical preparations.


Immunofluorescent study of alpha-foetoprotein (alpha-fp) in liver and liver liver tumours. I. Technique of alpha-fp localization in tissue sections.


Immunofluorescent study of alpha-foetoprotein (alpha-fp) in liver and liver tumours. II. Localization of alpha-fp in the tissues of patients with primary liver cancer (PLC).


Studies of carcino-fetal proteins: physical and chemical properties of human alpha-fetoprotein.


Lysosomes of rat liver and DAB carcinogenesis.


59Fe retention in sheep as a function of age and of artificially induced parasitism with Haemonchus contortus.


Betahistine, its metabolites and vascular responses in the forelimb of the dog.


Teratogenic effects of a chelating agent and their prevention by zinc. Ingestion of a chelating agent (ethylenediaminetetraacetic acid) by female rats during pregnancy impaired reproduction and resulted in congenitally malformed young. When ethylenediaminetetraacetic acid was fed from days 6 to 21 of gestation, all of the full-term young had gross congenital malformations. These effects were prevented by simultaneous supplementation with 1000 parts per million of dietary zinc.


Toxic factors in enzymes used in laundry products. Autolyzates of Bacillus subtilis used as enzymes in laundry products contain several potentially toxic substances.


Agglutinin interaction with embryonic and adult cell surfaces.


Light deprivation for early retinitis pigmentosa. A hypothesis.


Pseudomonas corneal ulcers in contact lens wearers. Epidemiology and treatment.


Ocular localization of sodium fluorescein. Effects of administration in rabbit and monkey.


Neuro-retinal "lentoids" contain no lens antigens. Immunofluorescent criteria.


Postoperative hepatic dysfunction. Additional contributing factors.


Treatment of hepatic coma.

Treatment of hepatic coma.


Size of the immunizing dose and postvaccination immunity to typhoid and paratyphoid B from the results of controlled field trials.


Gram-negative systemic bacteraemia in alimentary tract surgery.


The pathogenesis of arthritis associated with viral hepatitis. Complement-component studies.


Procion Navy Blue in pulp and periapical studies.


The oral manifestations of Hurler's syndrome.


Apical closure of nonvital teeth.

Apical closure of nonvital teeth.


The problems of third molars (wisdom teeth).


The ABC's of periodontics.

The ABC's of periodontics.


The future of professional liability insurance.


"What every dentist should know about TMJ dysfunction.


"Simplified retrograde endodontics.

"Simplified retrograde endodontics.


Types of dental group practices and methods for evaluation.


Pre-payment dental care policies and principles of acceptability.


Mandibular reconstruction with bone graft. Case report.


A double blind study of Lidocaine in Orabase.


Human secretory immunoglobulins. 4. Quantitation of free secretory piece.


Human secretory immunoglobulins. 3. Immunochemical and physicochemical studies of secretory IgA and free secretory piece.


Studies on endo-beta-acetylglucosaminidase, staphylolytic peptidase, and N-acetylmuramyl-L-alanine amidase in lysostaphin and from Staphylococcus aureus.


Elimination of genetic elements governing competence in transformation of Neisseria meningitidis by treatment with ethidium bromide and acriflavin.


Delayed hypersensitivity in the guinea pig immunized with killed tubercle bacilli in adjuvant. 1. Development of peritoneal cell migration inhibition, skin reactions and antibodies to tuberculin purified protein derivative.


Delayed hypersensitivity in the guinea pig immunized with killed tubercle bacilli in adjuvant. 2. The effect of tuberculin purified protein derivative concentration on the inhibition of peritoneal cell migration.


Enzymoserological relationships between Vibrio comma and other gram-negative organisms.


Blood-brain barrier studies in man using the double-indicator method.


Ion transport across intestinal mucosa of winter flounder, Pseudopleuronectes americanus.


Plasma thyroxine and thyroid-stimulating hormone levels during submaximal exercise in humans.


Interrelation of carbohydrate and palmitate metabolism in skeletal muscle.


Gamma-ray induced strand breakage of Bacillus subtilis DNA irradiated in vivo.


Search for antagonistic Actinomycetae in Libyan soils.


Evidence for the release at low salt concentration of a lipid-protein-carbohydrate complex from isolated envelopes and whole cells of a marine pseudomonad.


Studies on the stability of ascorbic acid. V. Critical micelle concentration of ascorbyl monofatty acid esters.


Studies on drug metabolism. XII. Activity of liver microsomal drug-metabolizing enzymes in human liver.


Ventricular aneurysms in acute experimental Chagas' myocardiopathy.


Prosthetic aortic valve replacement complicated by diphtheroid endocarditis and aortopulmonary fistula.


On the existence of prolactin in cyclostomes.


Comparison of immunologic and enzymatic methods for ceruloplasmin quantitation in Wilson's disease.


Neoplasms of the hematopoietic system in nonhuman primates: report of one spontaneous tumor and two leukemias induced by procarbazine.


Mason-Pfizer monkey virus isolated from spontaneous mammary carcinoma of a female monkey. I. Detection of virus antigens by immunodiffusion, immunofluorescent, and virus agglutination techniques.


Viral hepatitis, type B (MS-2 strain). Studies on active immunization.


Keratinization related to premitotic labeling and inflammation of gingiva and alveolar mucosa in rhesus monkeys.


Total parenteral alimentation in infants.


The binding capacity of thyroxine-binding globulin in cerebrospinal fluid.


Effect and side effects of diphenylhydantoin (DPH) in relation to plasma concentrations.


51Cr-EDTA as an alternative to phenol red in a rat test meal technique.


Accumulation of chorio-retinotoxic drugs in the foetal eye.


Micro-color test for assay of transmissible gastroenteritis virus-neutralizing antibodies.


Pigment variations in ultraviolet-treated strains of the blue-green alga Anabaena doliolum.


Immunochemical studies on the fibrin stabilizing factors from human plasma and platelets.


Effects of polyphosphate on the iron metabolism.


Studies on neutralization of L-asparaginase activity in vitro and in vivo.


Rh-immunisation and its prevention.

Rh-immunisation and its prevention.


Structural investigations on pancreatic alpha-amylase. The effect of urea on the ultraviolet absorption.


Increased levels of serum immunoglobulins G and M in women using intrauterine contraceptive devices.


Effect of the cereus lytic enzyme on the chemical bonds of the bacterial cell wall.


Placental transfer of C14-ethanol.

Placental transfer of C14-ethanol.


Frequency and distribution of Trypanosoma cruzi and Trypanosoma rangeli in the Republic of Panamá.


Physical separation of three soluble malarial antigens from the serum of chickens infected with Plasmodium gallinaceum.


Antibody response to invasive amebiasis in Durban, South Africa.


The medical student's view of alcoholism education.


Research training in alcoholism.

Research training in alcoholism.


Typhoid-paratyphoid fevers in infancy. Experiences with 35 cases.


Factors affecting behavior maintained by response-contingent intravenous infusions of amphetamine in squirrel monkeys.


Immunologic studies on human follicular fluid.


Reproductive patterns of three species of macaques.


Absorption of Hg-203-Cl2 from ligated intestinal segment in the rat in vivo.


BA and SH antigen in chronic hepatitis.

BA and SH antigen in chronic hepatitis.


The incidence of farmer's lung in Caithness.


Proliferation of hepatic parenchymal cells in immune-nephritic rats.


Dark and light somatotroph cells in the adenohypophysis of the mouse.


A history of Forane.

A history of Forane.


Seizures induced by pentazocine.

Seizures induced by pentazocine.


Systemic and regional blood flow changes during halothane anesthesia in the Rhesus monkey.


Further studies on African strains of Mycobacterium tuberculosis: comparison with M. bovis and M. microti.


Use of 103Ru-labelled tris (1,10-phenanthroline) ruthenium (II) chloride as a marker in digestion studies with sheep.


Reversibility of biliary cirrhosis due to bile duct obstruction.


Hormonal induction of alkaline phosphatase activity by an increase in catalytic efficiency of the enzyme.


Isomers of alpha-tocopheryl acetate and their biological activity.


Pineal and gonadal function in the rat following cervical sympathectomy.


Modification of the Grason-Stadler experimental rat chamber.


Surveys of radium contamination in aircraft instrument repair facilities 1966-1967.


Role of lateral inhibitory interaction in color coding.


The management of brittle diabetes mellitus in children.


Stability and control of diabetes mellitus in children.


Hypertension due to vascular lesions in neurofibromatosis.


Immunosuppressive antisera to soluble extracts from mouse and human lymphocytes.


Effect of urethan on calves inoculated with blind-passed bovine lymphosarcoma material.


Nutmeg as a narcotic. A contribution to the chemistry and pharmacology of nutmeg (Myristica fragrans).


Biological membranes.

Biological membranes.


Model reactions for the biosynthesis of Erythrina alkaloids.


Structure of the red eye pigments of the fruit fly Drosophila melanogaster.


Clinical importance of wavelengths in photocoagulation.


Sequence of events during rapid germination of spores of Bacillus cereus.


Mycoplasma caviae, a new species.

Mycoplasma caviae, a new species.


A structural gene for Neurospora crassa isocitrate lyase.


The relationship between M-antigen and opacity factor in group A streptococci.


Gonococcal arthritis. A survey of 54 cases.


Pathogenesis of Escherichia coli diarrhea.


"Alcalase" and serum proteins.

"Alcalase" and serum proteins.


On the kinin factors in rat pleural exudate.


Cyclophosphamide treatment of experimental allergic encephalomyelitis in Lewis rats.


Deficient IgA antibody responses to arsanilic acid bovine serum albumin (BSA) in neonatally thymectomized rabbits.


Successful 20-hr preservation of the primate liver by simple cooling.


Hemagglutination test for Chagas' disease with chromium chloride, formalin-treated erythocytes, sensitized with Trypanosoma cruzi extracts.


Facial skeletal changes after mandibular condylectomy in growing and adult monkeys.


Sipple's syndrome (pheochromocytoma and thyroid carcinoma) with bilateral breast carcinoma.


Biogenic substances in sediments and fossils.


American trypanosomiasis in British Honduras. IX. Development of Trypanosoma (Schizotrypanum) cruzi in Triatoma dimidiata (Hemiptera, Reduviidae), and a note on the occurrence of dividing trypomastigote forms in the gut of some of the naturally infected bugs.


Cutaneous leishmaniasis.

Cutaneous leishmaniasis.


Harvest, resuscitation, preservation, and orthotopic transplantttion of canine cadaver heart-lung allografts.


Specificity and structure of the myeloma protein produced by mouse plasmacytoma MOPC-460.


Some immunological properties of rabbit antiserum to mouse growth hormone.


Postoperative hepatic function tests.

Postoperative hepatic function tests.


Some effects of neuraminidase on the in vitro interactions between spleen and mastocytoma (P815) cells.


Effect of reduced food intake on thyroid cell height and goitrogenic response in rats.


Biological activity of megalomicin A phosphate, a water-soluble salt of megalomicin A.


Properties of a novel preparation of prostaglandin synthetase from sheep seminal vesicles.


Changes in phospholipid metabolism of a tumor target cell during a cell-mediated cytotoxic reaction.


Myocardial phospholipase A of microsomal and mitochondrial fractions.


Thermal properties of fructose-I,6-diphosphate aldolase from thermophilic bacteria.


Quantitation of antibodies to nucleic acids by angle radial immunodiffusion.


Evidence for a non-lysosomal alpha-mannosidase in rat liver homogenates.


The carbonic anhydrase in the rumen epithelial tissue of the ox.


Isolation of the thyroid peroxidase complex.


The value of long-term continuous oxygen therapy as a part of the treatment of chronic respiratory insufficiency.


Congenital listeriosis with ocular involvement.


The in vitro effects of pH and type of milk media on the growth of mastitis organisms as related to antibiotic sensitivity tests.


Research in strabismus.

Research in strabismus.


Biological divergences in south-central Bougainville: an analysis of blood polymorphism gene frequencies and anthropometric measurements utilizing tree models, and a comparison of these variables with linguistic, geographic, and migrational "distances".


The role of immunoglobulin A in endogenous bronchial defense mechanisms in chronic bronchitis.


Primary drug resistance: a continuing study of drug resistance in tuberculosis in a veteran population within the United States. IX. September 1969-September 1970.


The challenge and fascination of biliary tract surgery.


A large pharyngeal diverticulum.

A large pharyngeal diverticulum.


"Petit Mal Status" in adults.

"Petit Mal Status" in adults.


The ineffectiveness of diphenylhydantoin in preventing febrile convulsions in the age of greatest risk, under three years.


Differentiation of "Absence Status" and "Temporal Lobe Status".


Petit mal variant revisited.

Petit mal variant revisited.


Comment on "Petit Mal Variant Revisited".


Production and shielding of 15 MeV neutrons.


Dosimetry in neutron radiotherapy.

Dosimetry in neutron radiotherapy.


Collimation of 14 MeV neutron beams.

Collimation of 14 MeV neutron beams.


Radiotherapeutic requirements of 14 MeV fast neutron beams with respect to depth-dose and collimation.


The oxygen effect and radiotherapy with fast neutrons.


Response of a poorly reoxygenating mouse osteosarcoma to x-rays and fast neutrons.


RBE values of 15 MeV neutrons for effects on normal tissues.


Therapeutic margins in clinical radiotherapy with conventional types of radiations and their implications for neutron therapy.


Possible clinical models for quantitative comparison of effects of different radiations on tumours.


Evaluation of past experience with fast neutron teletherapy and its implications for future applications.


Clinical experience with fast neutrons from the Medical Research Council's cyclotron at Hammersmith Hospital.


Considerations in planning a clinical trial with fast neutrons.


Possibilities for the application of fast neutrons in Manchester.


Summary of general discussion on technical aspects of fast neutron therapy facilities.


Summary of general discussion on radiotherapeutic aspects of the clinical application of fast neutrons.


Concluding remarks on the application of fast neutrons in clinical radiotherapy.


Summary of general discussion on radiobiological aspects of fast neutrons in radiotherapy.


Physical characteristics of fast neutron beams.


Availability in the peccary pig of radionuclides in nuclear debris from the Plowshare Excavation Buggy.


Biological availability of radionuclides produced by the Plowshare Event Schooner. II. Retention and excretion rates in peccaries after a single oral dose of debris.


Allergic vascular purpura.

Allergic vascular purpura.


Isolation and characterization of multiple forms of hydroxyindole-O-methyltransferase.


Metabolic interlock. The role of the subordinate type of enzyme in the regulation of a complex pathway.


Isolation and characterization of electrophoretically homogeneous rabbit antihapten antibody populations. I. Separation and properties of homogeneous anti-p-azophenyltrimethyl-ammonium antibodies.


Alteration in the rheologic properties of Plasmodium knowlesi--infected red cells. A possible mechanism for capillary obstruction. Red cells parasitized by Plasmodium knowlesi concentrate within the microcirculation of many organs including cerebral capillaries in rhesus monkeys. The possibility that P. knowlesi could alter the rheologic properties of red cells so that they are trapped within capillaries was investigated in the present study. The viscosity of P. knowlesi-infected red cells suspended in Ringer's solution was increased at all shear rates at hematocrits above 30%. At moderate parasitemia the resistance to flow through 5 mu polycarbonate sieves was increased; at high parasitemia the pores were obstructed. Mature trophozoites caused more obstruction than young trophozoites (rings) at any given level of parasitemia. The reduction of deformability of red cells infected by schizonts of P. knowlesi was further demonstrated by their exclusion from rouleaux in a plasma suspension. Therefore, the red cells infected by P. knowlesi become less deformable, and this reduction in red cell deformability may explain the obstruction of cerebral capillaries.


The human alveolar macrophage: isolation, cultivation in vitro, and studies of morphologic and functional characteristics. Human alveolar macrophages were lavaged from surgically resected lungs and from lungs of normal subjects. Macrophages that had been purified by glass adherence were maintained in tissue culture for as long as 54 days. After 3-4 wk in vitro they underwent transformation into multinucleated giant cells. These aged cells had more than 30 times the phagocytic capacity that the same group of cells had had after 1 day in vitro. Phagocytosis of heat-killed Candida albicans was inhibited by iodoacetate, sodium fluoride, potassium cyanide, and low partial pressures of oxygen, suggesting that these cells require both oxidative and glycolytic energy sources for maximal particle ingestion. Alveolar macrophages and monocyte-derived macrophages killed Listeria monocytogenes with similar efficiency, but neutrophils were more efficient than either of the other cell types. Bacterial killing is probably not dependent upon myeloperoxidase in the monocyte-derived macrophage or in the alveolar macrophage since histochemical stains for peroxidase do not stain either cell type. C. albicans blastospores, which are killed by neutrophils and monocytes that contain myeloperoxidase, were not killed by human alveolar macrophages during the 4 hr of observation. Large cells with supernormal phagocytic capacity were recovered from patients with postobstructive pheumonia and from one patient with recurrent bacterial pneumonia, indicating that macrophage function can be altered in certain disease states. Human alveolar macrophages are unique human phagocytes in their dependence on an oxygen tension greater than 25 mm HG for maximal phagocytosis. Carbon dioxide tensions as high as 70 mm Hg did not alter phagocytosis when the pH of the medium was held constant. These data suggest that the increased susceptibility to pneumonia of patients with chronic bronchitis or atelectasis may be in part related to suboptimal phagocytosis by macrophages in areas of the lung with depressed oxygen tension.


The plasma estradiol as an index of fetoplacental function. A radioligand assay has been developed for the measurement of unconjugated 17beta-estradiol in as little as 0.01 ml of pregnancy plasma employing rabbit uterine cytosol as specific binder and activated charcoal as nonspecific absorbant. Large numbers of samples could be processed simultaneously at relatively little expense and results were obtainable within 3 hr. The procedure was sensitive to 1 ng/ml. No diurnal or positional variations were found. Values from 250 unselected normal patients showed a constantly rising mean plasma E(2) from 18 to 35 wk gestation from 4.5 to 14 ng/ml. From 35 to 40 wk, mean E(2) rose only to 15 ng/ml and the range of values increased substantially. When 22 normal pregnant subjects were followed serially, rising levels of plasma E(2) were found with no significant fall ever seen. By contrast, patients exhibiting fetal distress generally had falling or reduced E(2) levels. However, 3 cases of Rh isoimmunization had elevated or normal E(2) concentrations even after clearcut evidence of fetal demise. A decrease of 45% in E(2) concentration was associated with intraamniotic instillation of hypertonic saline prior to delivery supporting the view that placental conversion of maternal adrenal precursors is responsible for about half of the E(2) production in pregnancy. The postpartum clearance of endogenous E(2) was measured and found to fit a two compartment model with mean half-time of 22 min and 7 hr. Follicular phase levels of E(2) were attained by 35 hr postpartum. The concentration of unconjugated E(2) in pregnancy plasma correlated as well with the state of the placenta as other placental hormone measurements and holds promise of being a rapid, inexpensive, and reliable method of following patients with high-risk pregnancies in a variety of clinical settings.


The development of thyroid-stimulating hormone-releasing factor activity in the neonatal rabbit.


The antigenicity of human chorionic somato-mammotrophin and human growth hormone in the rhesus monkey (Macaca mulatta).


Glomerular basement membrane--reactive antibody in anti-lymphocyte globulin. Equine immunoglobulin was detected along the glomerular basement membrane of three human homograft recipients who had been treated with equine anti-lymphocyte globulin. Anti-lymphocyte globulins, given these patients, were obtained by immunization of horses with lymphocytes from human spleens and/or lymph nodes and contained glomerular basement membrane-reactive antibodies. Quantitative paired-label isotope experiments (in rats) demonstrated that 30-170 mug/ml of kidney-fixing antibodies were present in these preparations. The anti-lymphocyte globulins formed a line of identity with a sheep anti-human glomerular basement serum when reacted against collagenase-solubilized human glomerular basement membrane in double diffusion in agar. The renal fixation of these antibodies was blocked by absorption with human glomerular basement membrane, but not by buffy-coat leukocytes, indicating that they were directed specifically toward antigens in the basement membrane and were not cross-reacting anti-lymphocyte antibodies. Anti-lymphocyte globulin preparations for human use were studied for glomerular basement membrane-reactive antibodies by a direct immunofluorescent assay in rats. Anti-lymphocyte globulin from 13 of 20 horses, and 7 of 10 serum pools from horses immunized with lymphocytes derived from solid lymphoid organs (spleen, thymus, lymph node, tonsil), contained glomerular basement membrane-reactive antibodies. Sera from 18 horses injected with thoracic duct cells or cultured lymphoblasts had no glomerular basement membrane-reactive antibodies. An equine anti-human thymus serum containing glomerular basement membrane-reactive antibodies, which produced fatal glomerulonephritis in monkeys, was shown to cause both immediate and delayed glomerular injury in monkeys after intravenous injection. The reaction of this antibody with glomerular basement membrane in vivo was associated with little complement deposition in spite of the fact that the antibody could fix complement. This lack of glomerular complement fixation resulted from almost complete in vivo decomplementation of the monkeys receiving this anti-lymphocyte globulin.


Effect of dietary calcium, vitamin C and protein in development of experimental skeletal fluorosis. II. Calcium turnover with 45Ca; calcium and phosphorus balances.


Effect of dietary calcium, vitamin C and protein in development of experimental skeletal fluorosis. I. Growth, serum chemistry, and changes in composition, and radiological appearance of bones.


Nuclear and cell division in filamentous bacteria.


Independence of cell division and DNA replication in Bacillus subtilis.


Construction of the temporomandibular joint by transplanting the autogenous sternoclavicular joint.


Phylogenetic study of organic anion transfer from plasma into the liver.


Benign chondroblastoma: report of four cases with a discussion of the presence of ossification.


Anti-DNA activity in systemic lupus erythematosus. A diagnostic and therapeutic guide.


Inclusion of erythrocytes in the Ouchterlony test for Plasmodium gallinaceum precipitins.


American trypanosomiasis in British honduras. X. Natural habitats and ecology of Triatoma dimidiata (Hemiptera, Reduviidae) in the El Cayo and Toledo districts, and the prevalence of infection with Trypanosoma (Schizotrypanum) cruzi in the wild-caught bugs.


American trypanosomiasis in British Honduras. XI. Observation on the viability and morphology of Trypanosoma sigmodoni Culbertson 1941, in vitro.


Preliminary surveys for the prevalence of the thalassemia genes in some African populations.


Electromyographic abnormalities after intramuscular injections.


Colobomatous cupping of the optic disc.

Colobomatous cupping of the optic disc.


Studies on bacterial lipase. I. Nutritional requirements of Pseudomonas aeruginosa for production of lipase.


Studies on bacterial lipase. II. Study of the characteristics of partially purified lipase from Pseudomonas aeruginosa.


Learning: a comparative study of Lemur and Cercopithecus.


Extinction: a comparative primate study of Lemur and Cercopithecus.


Rank order in a captive female squirrel monkey colony.


Amygdalectomy and social behavior in the caged stump-tailed macaque (Macaca speciosa).


A comparison of the hemograms of Macaca mulatta, Macaca nemestrina and Papio anubis.


Colobomas in non-human primates.

Colobomas in non-human primates.


Pyocine typing as an epidemiological marker in Pseudomonas aeruginosa mastitis in cattle. Pyocine typing was used for the characterization of 134 Pseudomonas aeruginosa strains isolated from bovine mastitis. The scheme of Gillies & Govan (1966) was adopted with some modifications, and the procedure gave 89.6% typability. Pyocine type 1 strains were most commonly encountered and were followed in frequency by types 10 and 3. The introduction of two additional indicator strains allowed for division of these types into subtypes.In spite of some limitations, discussed in the paper, the pyocine typing scheme proved to be useful in ;marking' P. aeruginosa strains and in following their association with bovine mastitis in various herds.


Multiple ureteral diverticula.

Multiple ureteral diverticula.


Pseudomonas aeruginosa in a regional burns centre. The construction of a Regional Burns Centre in Pinderfields General Hospital, Wakefield, presented an opportunity to study Pseudomonas aeruginosa infection in patients with extensive burns. During the first year (Barclay & Dexter, 1968) a system of disinfection and bacteriological control created conditions permitting more detailed studies to be undertaken which resulted in a significant reduction of infection and cross-infection.


The serologic detection of cancer by immunodiffusion. The Lewis test.


The antigenicity of rat mammary tumours, induced by 7,12-dimethylbenz(alpha)anthracene.


Problems in the assessment of vitamin deficiency.


Role of adrenergic mechanisms in development and therapy of open-angled glaucoma.


Calcification and ossification induced experimentally.


Influence of antibiotic therapy on wound inflammation and sepsis associated with orthopaedic implants: a long-term survey.


Phaeochromocytoma of the bladder.

Phaeochromocytoma of the bladder.


Duck plaque virus-induced intranuclear inclusions in duck embryo hepatocytes.


Summary of recent abstracts. 3. Malaria.


Two-wave length visible cytospectrophotometry of nucleic acids and proteins in the motor and sensory neurons and their glial cell-satellites of rat spinal cord during corazol seizures.


Histochemical and biochemical studies of glycyl peptidase and glycyl arylamidase.


Histochemical study of the "adrenocortical lipid hyperplasia" induced in rats by aniline.


Albumin in epithelial cells of intrahepatic bile ducts in man.


Mystery virus from Lassa.

Mystery virus from Lassa.


Effect of aerobic and anaerobic growth on the cell wall of Staphylococcus aureus.


Nonglomerular renal disease produced in rabbits by immunization with homologous kidney.


Enzyme degrading Vibrio parahaemolyticus K15-antigen.


Preparation of cytoplasmic membranes of Staphylococcus aureus FDA 209P through protoplasts made with the L-11 enzyme and a preliminary analysis of membrane antigens.


Studies on immunity to megacin A: susceptibility of phospholipids to phospholipase A activity of megacin A.


The effect of a tubercle bacillary lipid on the clearance rate of colloidal carbon from the blood of rabbits.


Passive protection of mice against Pseudomonas aeruginosa by serum from recently vaccinated mice.


Nitrimidazine in the treatment of Trichomonas vaginalis vaginitis.


Sensitivity to antibiotics of gonococcal strains isolated from sailors at Rotterdam.


Jejunal diverticula.

Jejunal diverticula.


Listeriosis.

Listeriosis.


Rhesus isoimmunization after abortion.

Rhesus isoimmunization after abortion. Out of 177 Rh(D)-negative patients studied, 96 were successfully followed-up after spontaneous or therapeutic abortion. Rh antibodies were detected by the indirect Coombs test in two patients and by an enzyme technique only in a further seven, an overall incidence of 9.4%.The prophylactic use of anti-D immunoglobulin is now recommended for Rh-negative non-immunized patients undergoing abortion, but the dose could be less than 75 mug. The Kleihauer test was of no value in predicting the risk of isoimmunization.


Immunochemical properties of polysaccharide fractions of Bacillus cereus strains isolated from cases of food poisoning.


The action of metoclopramide on human gastroduodenal motility. The action of metoclopramide on gastroduodenal motility was studied in 23 subjects: eight symptomless volunteers and 15 patients with varying upper abdominal conditions. The response depended on the basal contraction pattern before the drug was given, irrespective of the clinical diagnosis. The size of the antral contractions was consistently increased and the duodenal contractions became more closely linked to those of the antrum. This demonstrates a mechanism for the increased rate of gastric emptying that is observed clinically after metoclopramide.


Synthetic thyrotrophin-releasing hormone in man.


An experimental examination of the scrapie agent in cell membrane mixtures. I. Stability and physicochemical properties of the scrapie agent.


Lactate accumulation in primate spinal cord during circulatory arrest.


Infectivity of Gnathostoma spinigerum larvae in primates.


Immunoglobulins in human schistosomiasis mansoni.


Comparison of Schistosoma haematobium, S. mansoni, and S. japonicum infections in the owl monkey, Aotus trivirgatus.


Residual ridge remodeling after tooth extraction in monkeys.


Interarticular disc in wide mandibular opening in rhesus monkeys.


Tubular arrays in the endoplasmic reticulum in human tumor cells.


Increase in capillary filtration rate resulting from reduction in the intravascular calcium ion-concentration.


Sclerocornea.

Sclerocornea.


Intraocular pressure variation during xenon and ruby laser photocoagulation.


Ocular effects of argon laser radiation. II. Histopathology of chorioretinal lesions.


Microbial contamination in ocular cosmetics.


Enzymatic inactivation of gentamicin C components by cell-free extract from Pseudomonas aeruginosa.


Possible detrimental effect of large doses of vitamin D in familial hypophysphatemic vitamin D-resistant rickets.


Norrie's disease: an X-linked syndrome of retinal malformation, mental retardation, and deafness.


Cortical patterns in two syngens of Glaucoma.


Oral regeneration in the ciliate Stentor coeruleus: a scanning and transmission electron optical study.


Investigation of periodontosis with periodontitis: literature survey and findings based on ABO blood groups.


A new method for intraerythrocytic cultivation of malaria parasites (Plasmodium coatneyi and P. falciparum).


Further studies on the effects of antipantothenates on malaria parasites (Plasmodium coatneyi and P. falciparum) in vitro.


Cryptosporidium wrairi sp. n. from the guinea pig Cavia porcellus, with an emendation of the genus.


Scanning electron microscope observations on some life history stages of Carchesium polypinum (Ciliata, Peritrichia).


Ultrastructure of Cryptosporidium wrairi from the guinea pig.


Observations on the survival of washed boar spermatozoa in different media.


Carcinogenesis in tissue culture. XV. Aggregate-forming capacity of rat liver cells. Comparison of untreated controls, cells transformed in culture, and tumor produced by backtransplantation.


The termination of immunological tolerance induced in adult C3H mice to RGG by RGG-protein complexes.


Modification of the vestibular function with betahistine HCl.


Viral hepatitis, type B (MS-2 strain). Detection of antibody after primary infection.


Cholecystography and nephrotoxicity.

Cholecystography and nephrotoxicity.


Transplantation antigens in rhesus monkeys.


Ribosomes, bound and unbound.

Ribosomes, bound and unbound.


Natural and experimental phagocytosis by erythrocytes in amphibians.


Reflexion of genetic differences by spermatozoan antigens.


Plasma equine gamma-globulin in tolerant adult dogs given anti-lymphocyte globulin.


Effect of C4 depletion on the utilization of the terminal components of guinea-pig complement by endotoxin.


Action of pyrimethamine and related drugs against Plasmodium knowlesi in vitr.


Diverticula of the walls of the Fallopian tubes.


Treatment of the umbilical cord of the newborn.


Serum concentration of human placental lactogenic hormone (HPL) in pregnancy complications.


Meningococci in vaginitis.

Meningococci in vaginitis.


Long-term therapy of Wilson's disease.

Long-term therapy of Wilson's disease.


Treatment of arterial insufficiency in the lower limbs by Hydergin. A double-blind, cross-over examination studied by plethysmography.


Long-term evaluation of pharyngo-esophageal diverticulectomy.


Bronchial lipoma. Report of two cases showing chondroitic metaplasia.


Genetic control of plasma cholesterol. Studies on squirrel monkeys.


Interaction of steroid hormones and histones.


Intensive treatment of acute leukaemia.

Intensive treatment of acute leukaemia.


Folic acid deficient gammopathy.

Folic acid deficient gammopathy.


Demonstration of antimyelin antibodies by immunofluorescence in Guillain-Barré syndrome.


Heavy chain typing in IgG monoclonal gammopathies with special reference to cases of serum hyperviscosity and cryoglobulinaemia.


The use of the indirect fluorescent antibody technique in assessing the activity of antilymphocytic sera and antilymphocytic globulins.


Epilepsy in cardiopulmonary bypass patients receiving large intravenous doses of penicillin.


The acute effects of experimental head injury on the vertebrobasilar circulation: angiographic observations.


Intracellular cation alterations following major trauma: effect of supranormal caloric intake.


Regulation of sodium excretion with special emphasis on the current status of natriuretic hormone.


Effect of estrogen on the serum cholesterol and triglyceride levels of prostatic cancer patients.


Gonorrheal urethritis.

Gonorrheal urethritis.


Immunological studies of histaminase for the identification of the type of blood. I. The purification of histaminase from human placenta.


Carotid sinus nerve stimulation in angina pectoris.


Post-traumatic alkalosis in young men with combat injuries.


Intravenous hyperalimentation. An important adjunct in the treatment of combat casualties.


Enzmatic and histologic effects of side-to-side portacaval anastomosis on the primate liver.


Measurement of blood losses before and after renal transplantation.


Safety with lasers.

Safety with lasers.


An evaluation of a method of quantitative radial immunodiffusion.


A comparison of methods for the molecular quantitation of the fourth component of human complement.


Mechanism of cryo-precipitation. I. Characteristics of a human cryoglobulin.


Some chemical and biological properties of the F'c fragment of human gamma-G globulins.


The effect of transplanted lymphoid cells, irradiation, and drug therapy on permanent immunologic tolerance.


Deficiency of C3 inactivator in man.

Deficiency of C3 inactivator in man.


IgA and other immunoglobulins from the European hedgehog.


Serum antigens of cattle. II. Immunogenetics of two immunoglobulin allotypes.


Immunoelectrophoretic studies of the California encephalitis virus group.


Kinetics of bovine complement. II. Properties of the lytic intermediate.


Presence in rabbit colostrum of IgA autoantibodies to a gastric antigen.


The immune response in the hamster. V. Biologic activities of 7S-gamma-1 and 7S-gamma-2 globulins.


Conversion of non-immune rabbit spleen cells by ribonucleic acid of lymphoid cells from an immunized rabbit to produce IgM and IgG antibody of foreign heavy-chain allotype.


Structure and synthesis of the thyrotropin-releasing hormone.


Synthetic thyrotropin-releasing hormone. Activity in men and women, specificity of action, inhibition by triiodothyronine, and activity orally.


Synthesis of the thyrotropin-releasing hormone.


Synthesis of analogs of the thyrotropin-releasing hormone and structure-activity relationship.


Synthesis of 1-amino-2-hydroxycyclopentanecarboxylic acid.


Synthesis and antimicrobial evaluation of some 5-(5-nitrofurylidene)rhodanines, 5-(5-nitrofurylidene)thiazolidine-2,4-diones, and their vinylogs.


Pathology of hereditary conditions related to retinal detachment.


Cephalic exchange transplantation in the monkey.


The effect of biliary tract pressure on bile flow, bile salt secretion, and bile salt synthesis in the primate.


Regulation of antibody formation against human serum albumin in rabbits.


Australia antigen in hepatitis among Swedish track-finders.


Preparation and chemical characteristics of endotoxic lipopolysaccharide from three strains of Sphaerophorus necrophorus.


Neisseria gonorrhoeae. Colony variation II.


Neisseria gonorrhoeae 3. Demonstration of presumed appendages to cells from different colony types.


Comparison between the sensitivity of the immunoelectroosmophoresis and the Ouchterlony gel-diffusion method in detecting Australia antigen.


Rosette formation in vitro between normal human lymphocytes and sheep erythrocytes.


Sensitivity and appearance of resistance to rifampicin in N. gonorrhoeae.


Identification and characterization of cholera exotoxin in culture filtrates of V. cholerae.


The fate of women with normal coronary arteriograms and chest pain resembling angina pectoris.


Effects of sublingually and intravenously administered nitroglycerin on the cardiovascular system of the dog.


Clonidine hydrochloride in the treatment of hypertension.


Preparation of 5-alkyltetrahydrofolic acids (dl-5-alkyl-5,6,7,8-tetrahydropteroyl-L-monoglutamic acids).


Evaluation of different scintillation cocktails for counting of tritiated ribonculeic acid separated by sucrose gradient centrifugation.


A fatal instance of juvenile diabetes mellitus.


Should every newborn infant be given a Coombs test?


Fertilization and early embryonic development subsequent to mating with TEPA-treated male mice.


The genetics of aldehyde oxidase in Drosophila melanogaster.


Transformation of Bacillus subtilis using hybrid DNA molecules constructed by annealing resolved complementary strands.


Attachment of ribosomes to membranes during polysome formation in mouse sarcoma 180 cells. Addition of nutrients to starved mouse S-180 cells leads to rapid conversion of ribosomal monomers to polysomes. During this process, a portion of the ribosomes originally found in the 17,000 g (10 min centrifugation) supernatant of cell lysates becomes firmly attached to structures sedimenting at 500 g (5 min centrifugation). Electron microscopy of sections of the intact cells showed the change from randomly distributed ribosomal particles to clusters. Association with membranes also became evident. The material sedimenting at 500 g comprised nuclei enclosed in an extensive endoplasmic reticulum (ER) network. This fraction prepared from recovering cells showed numerous ribosome clusters associated with the ER network. The appearance of many of these clusters indicated that the ribosomal particles were not directly bound to the membranes. RNase treatment released about 40% of the attached ribosomes as monomers, and ethylenediaminetetraacetic acid released 60% as subunits. It is suggested that during polysome formation a portion of the ribosomes becomes attached to the membranes through the intermediary of messenger RNA.


Results of thyroid and whole-body counting of some medical and paramedical personnel.


The effect of protein synthesis inhibition on the entry of messenger RNA into the cytoplasm of sea urchin embryos. Emetine is a potent inhibitor of protein synthesis in sea urchin embryos. At a concentration of the drug that rapidly inhibits protein synthesis in blastulae by 95%, uridine incorporation into RNA continues for more than 1 hr and presumptive histone messenger RNA is synthesized and transported into the cytoplasm where it is apparently associated with polyribosomes. Possible explanations of this result and its implications for the "informasome" theory of messenger transport in embryonic cells are discussed.


Influence of starch and nitrogen sources on ruminal microorganisms of steers fed high fiber purified diets.


Analysis of photoenzymatic repair of UV lesions in DNA by single light flashes. 8. Inhibition of photoenzymatic repair of UV lesions in E. coli DNA by caffeine.


Ionophoretic fractionation of Naja haje venom.


Studies of antigenic fractions in honey-bee (Apis mellifera) venom.


Micro-crenation of infected erythrocytes.


Morphological aberration of Trypanosoma (schizotrypanum) cruzi in the blood of white mice infected with strain BHC-10 from British Honduras.


The maintenance of Schistosoma mansoni and Plasmodium knowlesi in vitro in a continuous flow heart-lung apparatus.


Variant specific schizont agglutination antibodies in human malaria (Plasmodium falciparum) infections in Aotus.


An electron cytochemical study of glucose-6-phosphate dehydrogenase activity in malaria-infected erythrocytes.


Zonal centrifugation as a technique for the separation of malaria-infected erythrocytes.


Macrophage "activation" and the action of opsonizing antibodies in malaria.


Latent delayed hypersensitivity in malaria.


The passive transfer of variant-specific protection in malaria.


The effect of immuno-depression due to neonatal thymectomy on infections with Trypanosoma cruzi in mice.


Studies on genetic selection in a completely ascertained caucasian population. I. Frequencies, age and sex effects, and phenotype associations for 12 blood group systems.


Dogma disputed. Does reproductive compensation exist? An analysis of Rh data.


Studies on genetic selection in a completely ascertained caucasian population. II. Family analyses of 11 blood group systems.


Blood groups and personality traits.

Blood groups and personality traits.


Changes in serotonin release in hypothalamus during cooling or warming of the monkey.


Antimicrobial activity of the carbenicillin-gentamicin combination against gram-negative bacilli.


Application and hazards of total parenteral nutrition in infants.


Intravenous hyperalimentation: a review.


Enzymology of experimental liver disease in marmoset monkeys. II. Experimental hepatitis.


Rabbit platelet injury by soluble antigen and antibody. 3. Effects of the sodium and magnesium salts of EDTA and EGTA.


The evolution and spread of gentamicin-resistant Pseudomonads.


Subphrenic esophageal diverticulum: a case studied by cine-manometry.


Urinary excretion of liver antigens in experimental hepatic diseases of the rat.


Persistence of cerebral blood flow autoregulation following chronic bilateral cervical sympathectomy in the monkey.


A relationship between rat kidney mitochondrial swelling and glutaminase activation.


Elimination of 203Hg-methylmercury in man.


Synthesis and release of noradrenaline and dopamine from discrete regiions of monkey brain.


Urinary metabolites of 4-14C-progesterone in the baboon (Papio spp.).


Case report of neurofibromatosis.

Case report of neurofibromatosis.


Effect of soluble blood-group substances on the phagocytic function of blood leucocytes.


Resistance to intracellular infection in mice immunized with Toxoplasma vaccine and adjuvant.


Enamel conditioning for fluoride treatments.


Progression of pulpitis to necrosis and periapical disease in deciduous and permanent teeth of monkeys.


Preliminary result of a new protocol for the active immunotherapy of acute lymphoblastic leukaemia: inhibition of the immunotherapeutic effect by Vincristine or Adamantadine.


The corticosteroid-antibiotic dressing as a capping for inflamed dental pulps.


Quantitation of cryogenic brain lesions.


Hemolysis by asbestos.

Hemolysis by asbestos.


Bacteraemia in patients receiving human cadaveric renal transplants. During a two-year period (1966 to 1968) 70 patients were admitted to the Renal Transplantation Unit at St Mary's Hospital and 22 (31%) developed bacteraemia. Eighteen of the 25 episodes of bacteraemia occurred after transplantation and seven before. The typical clinical syndrome was uncommon and was only seen in 32% of cases. The presence of shock was not recognizable in the majority of patients probably because they were already receiving steroid therapy. The commonest infecting organism was Ps. aeruginosa (nine cases) and Gram-negative bacilli accounted for 72% of the bacteraemias. Staph. aureus was isolated in five patients.A likely source of the infecting organism was found in 80% of cases and the commonest source was the urinary tract. Serious complications were present in 13 episodes of bacteraemia, and these were peritonitis before transplantation, necrosis of the donor ureter, and severe rejection or polar infarction in the donor kidney after transplantation. Bacteraemia due to Gram-negative bacilli was associated with complications in 61% of cases. Ps. aeruginosa was almost invariably isolated from complicated cases though Esch. coli was only seen in simple cases. Antibiotic treatment was successful in 64% of cases but the cure rate was higher (86%) before transplantation than after transplantation (55%). Although the presence of complications did not affect the cure rate in Gram-positive bacteraemia, the cure rate in complicated Gram-negative bacteraemias was poor and associated with a mortality of over 70%. Patients receiving human cadaveric renal transplants are susceptible to all kinds of bacterial infection including bacteraemia, and the results of antibiotic treatment are dependent on both the nature of the infecting organism and the presence of serious underlying complications.


Preparation of antiviral sera for immunofluorescence on infected tissue culture. Antisera were prepared in rabbits against parainfluenza types 1, 2, and 3, respiratory syncytial, herpes, and adenoviruses, either by intranasal administration or by injection of virus grown in RK(13) cells. These antisera gave satisfactory results when used for immunofluorescence by the indirect method on virus in tissue culture. No absorption of the antiviral serum was carried out.


Pharyngo-esophageal diverticula and cricopharyngeal incoordination.


Diffuse esophageal spasm.

Diffuse esophageal spasm.


Surgical therapy of esophageal strictures.


Studies on cephalosporin therapy of experimental streptococcal and staphylococcal infections in monkeys.


Automated reagin test for syphilis in a multichannel blood grouping machine.


A bactericidal product obtained from a mutant of Escherichia coli.


The cerebrovascular accident.

The cerebrovascular accident.


Return to work after treatment of rheumatoid arthritis. This study reports on the prognosis for return to competitive work of men disabled by rheumatoid arthritis following an intensive in-patient rehabilitation program. Ninety-four men were followed up at a mean time of 3.5 years after discharge from the unit. Sixty-five per cent of the city dwellers and 50% of those living in small communities or rural areas were working either full-time or half-time. Other factors such as education, work background and geographical location influenced the success of return to work.In the city, 12 families (22%) were receiving welfare assistance prior to admission to the comprehensive rehabilitation program. At follow-up six of these families were off welfare and paying taxes. This group alone represented a minimum saving of $35,000 yearly to public funds.


Relationship of blood acetoacetate and 3-hydroxybutyrate in diabetes.


Severe diabetic ketoacidosis (diabetic "coma"). 482 episodes in 257 patients; experience of three years.


Taenia crassiceps: antigenic comparison of two larval strains.


Regulation of the sequencing in sterol biosynthesis.


Researches concerning hetero-hemagglutination in relation with age in humans.


Nitroblue-tetrazolium reduction by neutrophils of newborn infants in in vitro phagocytosis test.


The effect of minocycline on meningococcal nasopharyngeal carrier state in naval personnel.


Epidemiology of sulfonamide-resistant meningococcal infections in a civilian population.


Intratypic antigenic variation among strains of the group B arboviruses Murray Valley encephalitis and Kunjin.


Formamide as a stabilizing solvent in the colorimetric determination of DOPA oxidation products.


Isolation and DNA content of nuclei of Physarum polycephalum.


Factors stabilizing bull sperm cell volume and prolonging motility at high dilution.


Separation and in vitro growth of mammalian corneal epithelial and endothelial cells.


Mechanism of action of bioseimycin.

Mechanism of action of bioseimycin.


Widespread occurrence of inhibitors of melanoma tyrosinase in plant and mammalian tissues.


Effect of polyalkyl polyphosphonates on bone development in young rats.


Two cell-bound keratinases of Trichophyton mentagrophytes.


Treatment with glibenclamide in maturity-onset diabetes mellitus.


Glucose metabolism of the isolated eccrine sweat gland. I. The effects of mecholyl, epinephrine and ouabain.


Control of free fatty acids in human surface lipids by Corynebacterium acnes.


Antigens of human wart tissue.

Antigens of human wart tissue.


Marburg virus infection in monkeys. Ultrastructural studies.


Regulation of tyrosinase activity in mouse melanoma and skin by changes in melanosomal membrane permeability.


Carbenicillin--gentamicin.

Carbenicillin--gentamicin.


Optical activity of LSD-DNA mixtures.

Optical activity of LSD-DNA mixtures.


Development of a specific anti-leukaemic serum for the treatment of leukaemia in clinics.


Induction of specific autoimmune response to leukaemic cells in human leukaemia patient by chemically tagged normal "O" group white blood cells.


Health care systems: a must for health care in the 70's.


Jessie M.

Jessie M.


Regulation and characterization of acid and alkaline phosphatase in yeast.


The serology of Erwinia spp. lipopolysaccharides in relation to the taxonomy of the genus.


A statement: on the division of nursing, National Institutes of Health.


Asparaginase: molecular and enzymic requirements for use as an antileukaemia agent.


Vaccination of mice against Listeria infection.


The learning climate.

The learning climate.


Ischemia in primate spinal cord injury.

Ischemia in primate spinal cord injury.


Pulmonary cancer metastatic to the kidney--a common renal neoplasm.


Lucille Petry Leone-first Chief Nurse Officer.


The use of epsilon aminocaproic acid in sickle cell trait hematuria.


An overview of title II: nurse training.


Fluoroscopic retrograde pyelography.

Fluoroscopic retrograde pyelography.


National Institute of Mental Health.

National Institute of Mental Health.


Margaret McLaughlin leaves Washington...


Faye G. Abdellah: a new appointment.

Faye G. Abdellah: a new appointment.


Administrators take dim view of value of family meetings.


Prostaglandins and aspirin.

Prostaglandins and aspirin.


Clothing for long-stay patients.

Clothing for long-stay patients.


Renal handling of bicarbonate: effect of mannitol diuresis.


Effect of some chemical agents on renin activity in vitro.


Induction of ovulation in noncycling and hypophysectomized rhesus monkeys with various human gonadotropins.


Neutron dose distributions for californium-252 sources.


Appearance of monohydroxylated chlorpromazine metabolites in the central nervous system.


Canine histoplasmosis and blastomycosis in Monogalia County, West Virginia: prevalence of immunodiffusion reactors.


Carcinoma of the larynx. II. Treatment by  60 Co supervoltage irradiation.


LiF surface and depth dose measurements of megavoltage photon and electron beams.


Experimentally induced bovine infectious keratoconjunctivitis: effectiveness of intramuscular vaccination with viable Moraxella bovis culture.


Growth characteristics of scrapie agent-infected mouse brain cell cultures.


Alkaline phosphatase isoenzymes in the squirrel monkey (Saimiri sciureus).


Ureteral reflux in the squirrel monkey (Saimiri sciureus).


Hepatitis-associated antigen (HAA). 1. Quantitation by electrophoresis in antibody-containing agarose gel by use of a primary standard of purified HAA.


Determination of tissue calcium by nondestructive neutron activation analysis.


Isolation of free secretory component (S.C.) from human milk, determination of its molecular weight.


The first component of guinea pig complement: hemolytic assays with EA, EAC4 and with rat and guinea pig late acting components.


The preparation of tritium-labeled Fab antibody fragments.


Purification of the human complement protein C1s by affinity chromatograhy.


Surface area of the squirrel monkey in relation to body weight.


Maternal and foetal circulation of the placenta.


Thermoregulation in the squirrel monkey (Saimiri sciureus).


Fracture reduction using a biodegradable material, polylactic acid.


Modified healing in experimental mandibular fractures.


The 'overlap' method of comparing drugs.


Lessons from Marburg disease.

Lessons from Marburg disease.


Degeneration in the peripheral visual pathway of vitamin B12-deficient monkeys.


Some observations on the mechanism of tobacco amblyopia and its treatment with sodium thiosulphate.


CSF osmolality in ketoacidosis.

CSF osmolality in ketoacidosis.


Pericardial windows or pericardiocentesis for pericardial effusions.


Arylaminopeptidase activities in human cariogenic and non-cariogenic oral bacteria.


Prophylactic antibiotics in otolaryngologic surgery: a double-blind study.


Glucagon physiology and pathophysiology.


Genetics for ophthalmologists.

Genetics for ophthalmologists.


The men who created Intal.

The men who created Intal.


An epidemiological approach to chronic obstructive pulmonary disease and alpha antitrypsin in hospital patients: a tri-racial study.


Typhus verdict in American history.

Typhus verdict in American history.


Lactic acidosis.

Lactic acidosis.


Chikungunya virus in the Entebbe area of Uganda: isolations and epidemiology.


Ultrastructure of the bronchial epithelium of the pig.


The electrocardiogram, vectorcardiogram and spatiocardiogram in the Capuchin monkey (Cebus apella).


The blood supply to the lumbar vertebrae of the cat.


Studies on the seminal picture of simmental, friesian and balady bulls in Egypt.


Effect of harmine and harmaline hydrochloride on pregnancy in white rats.


Scoliosis in ducks.

Scoliosis in ducks.


The persistence of the dextrolateral Müllerian duct in hens (Gallus gallus) in the post-incubation period.


Snail problems in African schistosomiasis.


Onchocerciasis.

Onchocerciasis.


Investigation of homologous antibodies to an extract of rabbit dental pulp.


Dietary pulmonary hypertension.

Dietary pulmonary hypertension.


Assessment of propranolol in angina pectoris. Clinical dose response curve and effect on electrocardiogram at rest and on exercise.


The diagnosis of pemphigus.

The diagnosis of pemphigus.


Uveal colobomata and other anomalies in three generations of one family.


Morphological studies on fimbriae of Pseudomonas aeruginosa and Aeromonas hydrophila with special reference to their biological functions.


Factors affecting the outcome of treatment of pulmonary tuberculosis in sub-optimal conditions: an 18-month follow-up of 224 patients.


Two mutations giving low-level streptomycin resistance in Escherichia coli K 12.


Starch gel electrophoresis of fructose-6-phosphate phophoketolase in the genus Bifidobacterium. Three groups of nomenspecies of the genus Bifidobacterium were distinguished by the different mobility of their fructose-6-phosphate phosphoketolase in starchgel electrophoresis; there is apparently a close relatedness between electrophoretic type of phosphoketolase and habitat.


Effects of pH on transformation of Bacillus subtilis with single-stranded deoxyribonucleic acid. Variation in frequencies of transformation mediated by native and single-stranded DNA and its dependence on pH of the medium were investigated. The results indicate that the biological activity of deoxyribonucleic acid (DNA) of both configurations assayed in the presence of ethylenediaminetetraacetic acid (EDTA) increases as the pH of the transformation mixture is lowered from 7.7 to a maximum transformation frequency near pH 6.1. At this lower pH, native DNA transforms equally in medium with and without EDTA, and single-stranded DNA is 0.4 to 0.6 as active as native DNA in transforming Bacillus subtilis. A high efficiency of transformation with single-stranded DNA was observed for five markers in three recipient strains. The increased efficiency of native DNA appears to be caused by a lesser capacity of EDTA to bind magnesium at the lower pH. The increased efficiency of single-stranded DNA at pH below 7.0 results from decreased activity of a single-strand specific nuclease present in competent populations.


Relationship between competence for transformation of Bacillus subtilis with native and single-stranded deoxyribonucleic acid. The response of populations of Bacillus subtilis to both native deoxyribonucleic acid (DNA) and denatured DNA was investigated at maximal competence and at various times during the development of compentency. The results indicate that competence for transformation with native and denatured DNA increases and decreases simultaneously. Competition occurs between native and single-stranded DNA during transformation, and the same cells in a population can be doubly transformed by DNA molecules of both configurations.


Effect of glutamate on exogenous citrate catabolism of Neisseria meningitidis and of other species of Neisseria. Resting cell suspensions of Neisseria meningitidis group B (strain 2091) do not catabolize citrate as the sole substrate to an appreciable degree. When another substrate, such as glutamate, is also present to furnish energy for transport, citrate metabolism is greatly stimulated. Within limits, the amount of CO(2) produced from citrate is proportional to the amount of glutamate added. When the cells are disrupted, citrate is degraded at a rapid rate and the stimulatory effect of glutamate is completely eliminated. Pronounced stimulation of citrate metabolism by glutamate was demonstrated in 12 of 13 strains of N. meningitidis tested and only 1 of 6 strains of N. lactamicus. The remaining strains of N. lactamicus and one each of N. gonorrhoeae, N. flavescens, and N. flava did not utilize significant amounts of citrate in the absence or presence of glutamate. N. catarrhalis shared with Mima polymorpha and Moraxella glucidolytica a capability to catabolize citrate at a rapid rate without added glutamate. It is concluded that tests of glutamate-stimulated citrate metabolism may contribute to species characterization in the genus Neisseria.


Isolation and characterization of tricarboxylic acid cycle mutants of Bacillus subtilis. A technique was developed for the detection, on agar, of mutants of Bacillus subtilis that lacked a functional tricarboxylic acid cycle. Mutants devoid of detectable levels of aconitase, isocitric dehydrogenase, alpha-ketoglutarate dehydrogenase, succinic dehydrogenase, fumarase, and malate dehydrogenase have been isolated and characterized. Several mutants with conditionally expressible lesions, including a mutant with a heat-sensitive citrate synthase, have also been isolated. All of the mutants examined express all the biochemical markers normally absent in early-stage sporulation mutants except elastase, and some of these mutants sporulated nearly as well as the prototroph.


Studies on antibiotic synergism against enterococci. I. Bacteriologic studies.


Specific immunoassay for quantitative determination of human trypsin in intestinal content.


Repair of radiation damage to deoxyribonucleic acid in germinating spores of Bacillus subtilis. The repair of deoxyribonucleic acid (DNA) in germinating spores was studied in comparison with that in vegetative cells. Radiation-induced single-strand breaks in the DNA of spores and of vegetative cells of Bacillus subtilis were rejoined during postirradiation incubation. The molecular weight of single-stranded DNA was restored to the level of nonirradiated cells. The rate of the rejoining of DNA strand breaks in irradiated spores was essentially equal to that in irradiated vegetative cells. The rejoining in spores germinating in nutrient medium occurred in the absence of detectable DNA synthesis. In this state, normal DNA synthesis was not initiated. Very little DNA degradation occurred during the rejoining process. On the other hand, in vegetative cells the rejoining process was accompanied by a relatively large amount of DNA synthesis and DNA degradation in nutrient medium. The rejoining occurred in phosphate buffer in vegetative cells but not in spores in which germination was not induced. Chloramphenicol did not interfere with the rejoining process in either germinating spores or vegetative cells, indicating that the rejoining takes place in the absence of de novo synthesis of repair enzyme. In the radiation-sensitive strain uvs-80, the capacity for rejoining radiation-induced strand breaks was reduced both in spores and in vegetative cells, suggesting that the rejoining mechanism of germinating spores is not specific to the germination process.


Variation in the primary structure of Bacillus subtilis flagellins. The flagella derived from 18 strains Bacillus subtilis were tested for their reaction with antiflagellar filament antibody and antiflagellin antibody. On the basis of their reactivity, at least five serologically distinct classes could be identified. Peptide map analysis of tryptic digests of the subunit proteins were consistent with the immunochemical analysis. Large differences in sequence existed among proteins of the different classes; proteins within an antigenic group differed by only a few peptides. Furthermore, 9 of the 27 tryptic peptides resolved were common to flagellin proteins from all the classes examined. The relationship between antigenic specificity, variability in peptide pattern, and the conformation of the flagellin protein are discussed.


Amniotic and urinary oestriol assays in pregnancies complicated by rhesus immunization.


Structure and organization of mammalian stratum corneum.


Minor threonine dehydratase encoded within the threonine synthetic region of Bacillus subtilis. Challenging auxotrophs on metabolites that are precursors of a biosynthetic step involving a mutated enzyme has revealed a new class of suppressor mutations which act by derepressing a minor enzyme activity not normally detected in the wild-type strain. These indirect, partial suppressor mutations which allow isoleucine auxotrophs to grow on homoserine or threonine have been analyzed to determine their effect on enzymes involved in the biosynthesis of these amino acids. It has been found that one class of these suppressor mutations (sprA) leads to the derepression of homoserine kinase, homoserine dehydrogenase, and a minor threonine dehydratase that is not sufficiently active to be detected in the wild-type strain. The gene encoding this second threonine dehydratase activity has been found to be located between the structural genes for homoserine kinase and homoserine dehydrogenase. The results of these experiments indicate that plating of auxotrophs on precursors of a biosynthetic step involving mutated enzymes could prove to be a valuable method for the detection of regulatory mutants as well as a possible tool in studying the evolution of biochemical pathways.


Characterization of ribonucleic acid from visna virus. A single-stranded ribonucleic acid(s) has been isolated from purified virions of visna virus. It consists of two major components, namely 63S and "4S," under the conditions employed for ribonucleic acid (RNA) extraction. The 63S component can be converted to subunits by heat and dimethylsulfoxide treatments. Analyses by base composition indicate that the "4S" RNA isolated from visna virus is not a random breakdown product of the 63S component as a result of extraction, nor is it randomly derived from cellular RNA.


Transfection of Escherichia coli spheroplasts. I. General facilitation of double-stranded deoxyribonucleic acid infectivity by protamine sulfate. The addition of 25 mug of protamine sulfate per ml to lysozyme-ethylenediamine-tetraacetic acid spheroplasts of Escherichia coli stimulates transfection not only for T1 phage deoxyribonucleic acid (DNA; Hotz and Mauser, 1969) but also for the following phage DNA species: lambda, 10,000-fold to an efficiency of 10(-3) infective centers per DNA molecule; phiX174 replicative form, 300-fold to an efficiency of 5 x 10(-2); fd replicative form, 300-fold to 10(-6); T7, 300-fold to 3 x 10(-7). Three native phage DNA species were not infective at all in the absence of protamine sulfate but were infective in the presence of protamine sulfate with the following efficiencies: T4, 10(-5); T5, 3 x 10(-6); and P22, 3 x 10(-9). The effect of protamine sulfate is specific for double-stranded DNA. The application of infectivity assays to the study of phage DNA replication, recombination, prophage integration, prophage excision, and interspecies transfection are discussed.


Air embolism as a complication during parenteral alimentation.


Genito-urinary tuberculosis. A study of the disease in one unit over a period of 24 years.


Removal of lipids from human plasma low-density lipoprotein by detergents.


Unit study of exteroceptive inputs to claustrocortex in awake, sitting, squirrel monkey.


Prick-tests and serological tests in the diagnosis of allergic reactivity to enzymes used in washing products.


The price of progress: technology and the cost of medical care.


The price of "progress".

The price of "progress".


Subacute combined degeneration of the cord and the production of folic acid by intestinal bacteria.


The whole body autoradiographic studies on the distribution of  14 C-labeled new 1,5-benzothiazepine derivative ( 14 C-CRD-401) in mice.


Effects of in vivo and in vitro administered thyroxine on substrate metabolism of isolated rabbit ventricle mitochondria. II. Characteristics of oxidative phosphorylation in mitochondria of euthyroid, hyperthyroid and thyrotoxic rabbits.


Effect of peptide derivatives of thyroxine on the binding of 125-I-thyroxine to purified human thyroxine-binding globulin.


Retention of liver-specific antigen by hepatocytes in filter-well culture.


Antigens of mouse spleen cells: immunochemical analysis with heterologous rabbit antisera.


Studies of cryoprecipitates from synovial fluid of rheumatoid patients.


Catfish antibodies to blood group substances. I. Response of the Australian freshwater catfish, Tandanus tandanus (Mitchell), to the injection of human A, B and H secretor materials.


Inoculation of Streptococcus mutans and other bacteria into dental pulps of rats.


Distribution and disappearance rate of renin in man and dog.


Immunopathology of New Zealand Black mice treated with antilymphocyte globulin.


Increase in thyrotrophin secretion by rat pituitary autografts after infusion of hypothalamic extracts.


Relationship between total thyroxine and absolute free thyroxine and the influence of absolute free thyroxine on thyroxine disposal in humans.


The antigens and autoantigens of the seminal vesicle. I. Immunochemical studies on guinea pig vesicular fluid. Guinea pig vesicular fluid was characterized both biochemically and immunologically. Biochemical analyses showed this fluid to be homogeneous by ultracentrifugal analyses, revealing a single boundary with a sedimentation coeflicient of 1.5 S. In contrast, electrophoretic separation methods revealed six components, of which three were major components, of approximately equal proportions. They were termed I, II, and III. One of these components (II) was shown to be strongly antigenic in heteroimmunization, whereas components I and III failed to show any antigenicity, even after diverse attempts. This antigen (component II) was found to be highly tissue specific and species specific. Through procedures of isoimmunization, component II was also found to be immunogenic, giving rise (in male animals) to autoantibodies, A high proportion of injected guinea pigs showed positive skin tests and many revealed tissue lesions when the seminal vesicles were examined histologically. It is therefore concluded that experimental autoimmune disease of the seminal vesicle has been induced.


In vitro studies of complement function in sera of C4-deficient guinea pigs. In vitro studies were performed utilizing sera from a strain of guinea pigs with a total absence of hemolytically active C4. Previous studies in these animals have demonstrated normal complement-dependent inflammatory reactions, suggesting that they are able to bypass their deficiency of C4. In vitro studies with C4-deficient serum also indicate normal activation of late-acting C components. Thus, endotoxin was capable of fixing normal amounts of the late components of complement (C3-9) in these sera, but did not fix C1 and C2. Antigen-antibody complexes fixed both early and late components of complement, although components beyond C4 were fixed less efficiently than in normal sera. Therefore, both in vivo and in vitro evidence indicates that the C4-deficient guinea pigs possess an alternate pathway for activation of late-acting complement components. Antigenic analysis of C4-deficient serum utilizing both guinea pig anti-C4 antibody and rabbit anti-C4 antibody suggests an absolute deficiency of C4-like molecules. Sera from animals with C4-deficiency were found to have one-half the normal level of C2. Sera from five of eight animals tested had 10-20% normal C1 activity. C3-9 assayed as a complex was normal.


Immune response to chemically modified flagellin. I. Induction of antibody tolerance to flagellin by acetoacetylated derivatives of the protein. Flagellin (mol. wt. 40,000) from S. adelaide organisms was acetoacetylated to varying extents with diketene (acetoacetic anhydride). Chemical studies demonstrated that the amino groups of flagellin were more readily acetoacetylated than the hydroxyl groups. Several antigenic tests revealed that as flagellin was acetoacetylated to increasing extents there was a steady decline in the affinity of the molecule for anti-flagellin antibodies. Loss in antigenic activity following acetoacetylation was not related to the number of acetoacetyl groups attached but was determined by the type of residue substituted. Reactive lysine residues were much less important anti-genically than easily substituted hydroxyl groups. Acetoacetylation very readily destroyed the antibody-forming capacity of flagellin in rats. This fall in immunogenicity was related to the antigenic activity of the preparations. In fact, only a 40% reduction in the antigenic activity of flagellin produced a 90-95% reduction in primary antibody formation. The more heavily acetoacetylated flagellins produced no detectable antibody and, in fact, rendered adult rats tolerant (in terms of antibody formation) to a subsequent challenge of flagellin. Tolerance was induced by acetoacetylated flagellins which had drastically reduced affinities for anti-flagellin antibodies. These results were interpreted as indicating that the affinity of antigen for receptors on cells appears to be of crucial importance in determining whether antibody formation or immunological tolerance (antibody suppression) occurs.


Characterization of immunoglobulin structures from the surface of chronic lymphocytic leukemia cells. Chronic lymphocytic leukemia cells with relatively large amounts of mu and kappa immunoglobulin structures on the surface, and apparently very small amounts intracellularly, were subjected to homogenization or washing after freezing and thawing. After a light centrifugation, which sedimented the nuclei and unbroken cells, most of the immunoglobulin structures were found in the supernatant. Ultracentrifugation, which was performed to remove the membranes from the supernatant, sedimented only half the amount of the immunoglobulin structures. By sucrose density gradient ultracentrifugation and Sephadex G-200 filtration, the unsedimented immunoglobulin structures were shown to consist of 7S IgM and free kappa chains. About 80,000 7S IgM molecules were calculated to be present on each cell. The amount of kappa chains not associated with IgM was estimated to be equal to the amount of kappa chains in IgM. Inhibition of passive hemagglutination was used to detect and quantitate the immunoglobulin structures.


Histology of Rhodnius prolixus infected with Trypanosoma rangeli.


Antibodies to polynucleotides in human sera: antigenic specificity and relation to disease. The specificities of anti-polynucleotide antibodies found in human sera were studied using several immunological procedures. Anti-native DNA (NDNA) antibodies and certain anti-double-stranded RNA (DSRNA) antibodies were found to react with single-stranded DNA (SDNA), and anti-NDNA antibodies were observed to react more avidly with SDNA than with NDNA in most sera tested. Antibodies to NDNA showed no preferential reactivity with NDNA or SDNA derived from mammalian tissue, bacterial, or viral sources. Precipitating antibodies reactive with individual bases, with common determinants of bases, and with common determinants of SDNA and NDNA were detected utilizing synthetic polydeoxyribonucleotides. Antibodies to DSRNA were also heterogeneous and reactive with both Poly A . Poly U and Poly I . Poly C in addition to reactivity with Poly A and SDNA. In contrast, antibodies to a ribonucleo-protein determined by hemagglutination and by precipitation showed no reaction with NDNA, SDNA, or DSRNA. Serial studies of serum specimens from patients with systemic lupus erythematosus (SLE) indicated that anti-NDNA antibodies were closely associated with disease activity. Titers of antibodies to SDNA or DSRNA were also frequently increased during these periods but in addition showed peaks during quiescent periods. Anti-NDNA antibodies were detected in most patients' sera at sometime during the course of the disease. Three patients were observed with active SLE, who did not develop anti-NDNA antibodies, even in the presence of severe renal disease. Evidence that other antigen-antibody systems may also play a role in the pathogenesis of the renal disease was particularly apparent in these patients. Anti-ribonucleoprotein antibodies were not well correlated with the peaks of antibody activity of other polynucleotide antibodies, suggesting that an independent immunogen was responsible for induction of these antibodies. The close association of certain populations of anti-polynucleotide antibodies during the course of active SLE, the presence of cross-reacting antigenic determinants of SDNA, NDNA, and DSRNA, the preferential avidity of anti-NDNA antibodies for SDNA, and the frequent increase of anti-SDNA antibodies in SLE and other diseases associated with active tissue destruction suggest that SDNA is a ubiquitous antigen that may stimulate the formation of antibodies reactive with a variety of polynucleotides.


Determination of activity of cholinesterases.


Determination of histidine decarboxylase activity.


Determination of amine oxidases.

Determination of amine oxidases.


The immunochemical requirements for specific activity and the physiochemical properties of eel anti-human blood-group H(O) 7 S globulin.


Effects of neonatal exposure to isophilic and-or heterophilic antigens on immune responses of adult rats.


Determination of total and free serum thyroxine in thyroid diseases.


The ontogenesis of hemoglobins in Macaca nemestrina.


Studies on a protein isolated from livers of diabetic and fasted rats.


Congenital abnormality of the prothrombin molecule (factor II) in four siblings: prothrombin Barcelona.


The group-specific component (Gc) in chimpanzees.


The distribution of autonomic post-ganglionic nerve fibres to the lacrimal gland in monkeys.


Responsiveness of arterial smooth muscle to noradrenaline after long periods in Ca-free solution containing EDTA at low temperature.


Cromolyn sodium (Intal), a new antiasthmatic drug.


Cytoplasmic localization of ribonucleic acid synthesis induced in the cells infected with some group A arboviruses.


Engineering and physiological considerations of direct capacitor-discharge ventricular defibrillation.


Preliminary studies on antithymocyte globulin.


Delayed type hypersensitivity to tuberculin in young germ-free fowls.


Standardization of agglutinating antigens for the diagnosis of brucellosis.


Purification and in vitro properties of antihuman and anti-macaca thymocyte IgG, comparison of prolonged and pulse immunization, and the use of macaca thymocytes to conserve human antigen.


Prognostic value of quantitative determination of rhesus antibody (anti-D) in maternal serum and amniotic fluid.


Studies on diamine oxidase activity during pregnancy.


Hyperactivity and minor physical anomalies in elementary school children.


Use of the laboratory in a teaching hospital. Implications for patient care, education, and hospital costs.


A partial amino acid sequence in the heavy chain of a rabbit antibody to group C streptococcal carbohydrate.


Human pancreatic enzymes. Isolation and properties of a major form of chymotrypsin.


Effect of chemicals on the early stages of tobacco mosaic virus protein polymerization. Osmotic pressure and sedimentation velocity techniques have been used to investigate the effect of KSCN, thiourea, EDTA, acetamide, and sucrose on the 53,000 molecular weight A protein at pH 6.5-7.0. In the presence of all the compounds except thiocyanate, the number average molecular weight lies between 50,000 and 56,000 which corresponds to a trimer of three chemical subunits. In the presence of thiocyanate, the molecular weight decreases initially sharply with increasing concentration of thiocyanate to 0.1 M, then dissociation proceeds with less efficiency with increasing concentration of KSCN until a molecular weight close to a monomer (21,700) is obtained at 0.59 M KSCN. Sedimentation data agree with osmotic pressure data since, in the absence of thiocyanate, the measured values of S(20,w) indicate that the favored state is that of a trimer. In the presence of thiocyanate, however, S(20,w) decreases with thiocyanate concentration to S(20,w) = 1.9, which is the value reported for the monomer.


Sedimentation of DNA released from Chinese hamster cells. Under high pH and high salt conditions, Chinese hamster cells lyse and release a DNA-containing material of large molecular weight. With increasing lysis time, a smaller material is resolved from the large one. Relative to T4 DNA, the smaller is estimated to be approximately 2 x 10(8) daltons (number average). From a comparison of radiation data, the target size of the larger is about 15 times that of the smaller (probably a lower limit estimate). In addition to concentration of alkali, temperature, and time of lysis, the resolution of the smaller from the larger material is shown to be affected by other factors. Three of these are: fluorescent light exposure during lysis, X-irradiation before lysis, and incorporation of actinomycin D before lysis. All of these treatments result in degradation of the smaller molecules if large enough exposures are used. The sedimentation patterns of both DNA materials are strongly speed dependent. This probably results from changes in molecular conformation and concomitant increases in viscous drag with speed. The speed dependence differs qualitatively for the two materials, an observation which suggests that they differ in ways in addition to size.


Regional myocardial blood flow in the dog studied with radioactive microspheres.


An investigation into the clinical effects of fast neutrons. Methods and early observations.


School-children with perceptive deafness.


Rapid separation of generator-produced gallium-68 from EDTA eluate.


The significance of rises in rhesus antibody titre following amniocentesis.


The effect of ethyl alcohol on prostaglandins E 2  and F 2   induced uterine activity in pregnant women.


Inhibition of bacterial DNA replication by 6-(p-hydroxyphenylazo)-uracil: differential effect on repair and semi-conservative synthesis in Bacillus subtilis.


A further study on the family with anomalous inheritance of haptoglobin types.


Needed research on demographic aspects of the black community.


Endocervical Neisseria meningitidis with meningococcemia.


Erythrocyte 2,3-diphosphoglycerate in adaptive red-cell-volume deficiency.


Meningococcus and gonococcus: never the Twain--well, hardly ever.


Hyperalimentation--a case report of its use in perforated necrotizing enterocolitis.


Listeriosis in Auckland.

Listeriosis in Auckland.


Comparative studies on anti-Ag sera in immunodiffusion and in passive hemagglutination methods.


Serum alpha-1 acid glycoprotein concentration in pregnancy and with oral contraception.


Anesthetic management for fetal operation in the subhuman primate.