Chemical modification of bacterial alpha-amylase by monoiodoacetate.


Regulation of isoleucine-valine biosynthesis in Pseudomonas aeruginosa. I. Characterisation and mapping of mutants.


Regulation of isoleucine-valine biosynthesis in Pseudomonas aeruginosa. II. Regulation of enzyme activity and synthesis.


Successful treatment of osteomyelitis caused by Pseudomonas aeruginosa. A case of generalized osteomyelitis due to Ps. aeruginosa is described. The condition had failed to respond to therapy with almost all antibiotics available but responded to long-term therapy with carbenicillin in very large doses, and it is suggested that this is now the treatment of choice for severe infections due to this organism.


Isozyme variations in human cells grown in vitro. 3. Hormonal influence on amino acid naphthylamidases in HeLa cells.


Immediate function with survival after left lung autotransplantation and contralateral pulmonary artery ligation in the baboon.


Separation of neuronal and neuropil cell fractions; a modified procedure.


One way to do experiments on gene conversion? Transfection with heteroduplex SPP1 DNA.


Production of antibiotics by geophilic dermatophytes. I. Screening test of antibacterial activity.


Virus-like particles in systemic lupus erythematosus.


Specific uptake of homologous DNA accompanying transformation in Neisseria meningitidis.


Serotyping of Pseudomonas aeruginosa. 2. Results of an O group classification.


The effect of bacterial growth on the hydrogen ion concentration in urine.


Purification, antibody activity and ultrastructure of secretory and high-polymer IgA.


Distribution of organ antigens on thyroid cell membranes as examined by the immunofluorescence test.


Studies of the immune response in gonococcal infections with various serological techniques.


A radial immunodiffusion test for quantitative assay of influenza A antibodies.


Effect of temperature and inhibitors on serotonin-14C release from human platelets.


Effects of calcium and adenosine triphosphate on volume of human red cell ghosts.


Venous responses to prostaglandin F2 alpha.


Interaction of hormones and blood proteins. Some theoretical considerations about the precipitation reaction of thyroxine.


Methodology of neurophysiological research in anesthesiology.


Effect of foreign compounds on liver microsomal enzymes.


Isoniazid-resistant mutants of Mycobacterium tuberculosis H37RV: uptake of isoniazid and the properties of NADase inhibitor.


Profiles of soluble protein during sporulation of Bacillus subtilis.


Immunofluorescence examination of typical Staphylococcus aureus variants and L-forms for clumping factor.


Lysogenic conversion of Rhizobium trifolii.


Nature and properties of a cytolytic agent produced by Bacillus subtilis.


Stress and behavior.

Stress and behavior.


An evaluation of the single-channel autoanalyzer in the quantitation of anti-Rh-o.


Prevention and correction of deformity after severe burns.


The 'naturally occurring' anti-E.

The 'naturally occurring' anti-E.


A further examination of the survival of pathogenic bacteria in cattle slurry.


The cost of caring.

The cost of caring.


Biochemical method of cancer control. L-asparaginase in cancer chemotherapy. A review.


Epizootiology of listeric infection in sheep.


Histochemical differentiation of peroxidase-mediated from tyrosinase-mediated melanin formation in mammalian tissues. The biologic significance of peroxidase-mediated oxidation of tyrosine to malanin.


Crystallization and reaction mechanism of yeast phosphoglucomutase.


Isolation of a DNA-nicking enzyme from phage lambda-Ci-infected Escherichia coli.


Presence of antioxidant materials in bacteria.


Properties of light particles produced during growth of Type 4 adeno-associated satellite virus. Adeno-associated satellite virus type 4, obtained by repeated undiluted passage, failed to produce distinct bands at the expected density of 1.43 g/cm(3) after density gradient centrifugation in CsCl. This phenomenon occurred regardless of the hemagglutinating activity of the starting material. Sharp bands were found at a density of 1.34 to 1.35 g/cm(3). These bands contained adenovirions and numerous satellite particles. These latter particles could be distinguished by electron microscopy from standard dense satellite particles by their flattened profiles and deep penetration of negative stains. Dense bands of satellite virus at 1.43 g/cm(3) were constantly observed when the inoculum was comprised of highly diluted seed virus. Light satellite particles had a particle to HA ratio comparable with dense particles, but possessed low infectivity. Measurements of contour lengths of extracted deoxyribonucleic acid (DNA) indicate that light particles contain only a small amount of DNA, possibly less than 0.5 x 10(6) daltons, compared to 1.4 x 10(6) for the complete satellite DNA molecule.


Expanding horizons in medicine.

Expanding horizons in medicine.


Prenatal genetic diagnosis. 3.

Prenatal genetic diagnosis. 3.


Standarization of anti-D for use in the prevention of Rh sensitization.


Progress report of the Injury Control Research Laboratory of the Public Health Service.


Effects of foods on oral bacterial populations in man and animals.


Superior colliculus cell responses related to eye movements in awake monkeys. Single cell responses were recorded from the superior colliculus of awake monkeys trained to move their eyes. A class of cells that discharged before eye movements was found in the intermediate and deep layers of the colliculus. The response of the cells was most vigorous before saccadic eye movements within a particular range of directions. These cells had no visual receptive fields, and visually guided eye movements were not necessary for their discharge, since they responded in total darkness before spontaneous eye movements and vestibular nystagmus.


The effect of nitroglycerin on coronary blood flow and the hemodynamic response to exercise in coronary artery disease.


Studies of the coronary microcirculation of the cat.


The neurological basis of introversion-extraversion: Gray's Theory.


Possible inhibitory effect of teichoic acid on Bacillus subtilis transfer ribonucleic acid. 1. tRNA of Bacillus subtilis was found to be variably contaminated with membrane teichoic acid. 2. Samples with high contents of teichoic acid showed no accepting activity for tRNA(Phe) and tRNA(Tyr). 3. Removal of teichoic acid restored accepting activity and fractions containing teichoic acid, separated on Sephadex G-150, inhibited the charging of tRNA(Tyr). 4. The presence of teichoic acid did not inhibit the charging of tRNA(His).


Tryptophan catabolism during sporulation in Bacillus cereus. 1. Two intermediates of tryptophan catabolism were isolated from a sporulating culture of Bacillus cereus and identified as anthranilic acid and kynurenine by their spectral properties. 2. During sporulation the rate of formation of anthranilic acid and kynurenine by whole cells increased and reached a maximum at the pre-spore stage. 3. The specific activities of tryptophan pyrrolase and formylase also increased during sporulation and exhibited a maximal activity at the pre-spore stage. 4. Kynureninase activity reached a maximum during early stages of sporulation and then started to decline. 5. There was a net increase in the activity of tryptophan pyrrolase when cells were grown in the presence of l-tryptophan or dl-kynurenine. 6. The cultures exhibited the maximal activity of kynureninase 2h earlier in the presence of dl-kynurenine whereas l-tryptophan delayed the appearance of the maximal activity by 2h. 7. The omission of glucose from the medium had no effect on the pattern of development of tryptophan pyrrolase during growth and sporulation. 8. On the addition of tryptophan to a chemically defined medium no significant change in the pattern of development of tryptophan pyrrolase was observed.


Antigenicity of proteinases from Streptomyces griseus (pronase). The five pronase fractions, A(1), A(2), B, C (trypsin-like), and D (elastolytic), obtained by ion-exchange chromatography, were found to be antigenically distinct. Antibodies to pronase inhibited the enzymic activity of each of the enzyme fractions. Pronase trypsin and bovine trypsin, although resembling each other in enzymic activity and in amino acid sequence around their active sites, did not cross-react antigenically with, nor was their enzymic activity inhibited by, the respective homologous antibodies. Inactivation of pronase trypsin by complexing with soya-bean inhibitor AA, was not associated with a decrease in capacity to precipitate with its antibody. It is assumed that the antigenic sites are located far enough from the catalytic site of the enzyme to allow it to precipitate immunologically even when the catalytic site was blocked.


The isolation of polysomes from rat liver. Contamination of polysomes by ribonucleoprotein of nuclear origin.


The intermediate in the degradation of DNA alkylated with a monofunctional alkylating agent.


Action of crude bacterial alpha-amylase on tropocollagen.


Modification of carboxyl groups of transferrin.


Chromosomes of Callithricidae with special reference to an XX-'XO' sex chromosome system in Goeldi's marmoset (Callimico goeldii Thomas 1904).


Cerebral fissural patterns in platyrrhine monkeys.


Composition of milk from talapoin monkeys.


Vocalizations of East African monkeys. I. Red colobus.


Deviating segregations in families with elliptocytosis II (Pedigrees de M., v. B., Ri., Mo. and v. Ac.).


Concerning the question of individual constancy and individual specificity of three serum enzyme activities (serum cholinesterase EC 3.1.1.8, ceruloplasmin EC 1.10.3.2, and alkaline phosphatase EC 3.1.3.1).


Acid hydrolases of the retinal pigment epithelium.


An analysis of retinal receptor orientation. I. Angular relationship of neighboring photoreceptors.


The maintenance of sexual receptivity by adrenal androgens in female rhesus monkeys.


Annual changes in the menstruation of rhesus monkeys.


Abrogation of immunologic unresponsivenessy whole molecules and fragments of cross-reacting immuoglobulins.


Further studiesn guinea pig skin sensitizing activities of human myeloma proteins.


A type-specific immunodiffusion technique for the California encephalitis virus group.


Vibrio cholerae: production of toxin by a nonpathogenic strain.


Immunization against Brucella infections: immune response of mice, guinea pigs, and Cynomolgus philipinensis to live and killed Brucella melitensis strain Rev. I administered by various methods.


Meningitis and bacteremia after ventriculoatrial shunt-revision: isolation of a lecithinase-producing Bacillus cereus.


Uveal coloboma and true Klinefelter syndrome.


Hereditary macular coloboma.

Hereditary macular coloboma.


Trapping of unreplicated phage DNA into spores of Bacillus subtilis and its stabilization against damage by 32P decay.


A virus induced soluble antigen associated with potato virus-X infection.


Requirement of cellular synthesis for Kilham rat virus replication.


On visna virus: purification and nucleic acid content.


An antigenic analysis of potato virus X and of its degraded protein. I. Evidence for and degree of antigenic disparity.


Tissue injury in lymphocytic choriomeningitis viral infection: virus-induced immunologically specific release of a cytotoxic factor from immune lymphoid cells.


Reversion of rough (R) to smooth (S) forms in Brucella abortus. II. Comparison of the SII form obtained after reversion with the initial SI form, as well as RI and RII rough variants.


Catheter placement in infants needing total parenteral nutrition utilizing common facial vein.


Immunological control of Pseudomonas infection in burn patients: a clinical evaluation.


Fate of intravenously administered zinc chelates in man.


Experience in the treatment of burns in an underdeveloped country.


Deoxyribonucleic acid base composition of Rothia dentocariosa as determined by thermal denaturation. The moles per cent guanine plus cytosine (GC) of 10 filamentous strains of Rothia dentocariosa ranged from 65.4 to 69.7. Major differences were not observed in the base composition of a filamentous form (69.7 moles% GC) and its coccal variant (68.0 moles% GC).


Isolation and characterization of thymineless mutants from ultraviolet-sensitive Bacillus subtilis strains. Thymineless mutants of Bacillus subtilis 168 ind, hcr-9 were isolated by using trimethoprim. These and other Thy(-) strains differed drastically from Thy(+) ones in their patterns of [(3)H]thymidine uptake and growth in trimethoprim-containing medium. Transformation was negligible between most mutants derived from the ultraviolet-sensitive strain 168 ind, hcr-9 but significant between 168 ind, thy and these mutants. The latter and these new mutants all grow in the presence of trimethoprim plus thymidine or thymine and fail to grow if thymine or thymidine is omitted.


Uptake of synthetic polynucleotides by competent cells of Bacillus subtilis. A survey was made of the capacity of competent cells of Bacillus subtilis to take up synthetic polynucleotides. Polydeoxyribonucleotides but not polyribonucleotides are taken up by the cells. Both types of polynucleotide failed to compete with transforming deoxyribonucleic acid in the transformation assay whereas both stimulated amino acid incorporation. The latter phenomenon appears to be nonspecific as there is no correlation between the codon composition of the polynucleotides employed and the amino acids whose incorporation was stimulated.


Nucleic acid homology in the genus Mycobacterium. Immobilized deoxyribonucleic acid (DNA) preparations from eight species of mycobacteria were reacted with labeled reference DNA from Mycobacterium tuberculosis and M. kansasii. All mycobacteria showed some degree of homology. Immobilized DNA from Pseudomonas multivorans, which has a guanine plus cytosine content within the range established for the mycobacteria, showed no significant binding with either reference system. Relative per cent binding and thermal stability of bound DNA were used as parameters for determining degrees of relatedness among members of the genus. When these relationships were compared with those established by numberical taxonomic methods, a high correlation was found.


Analysis of the multiseptate potential of Bacillus subtilis. Bacillus subtilis was grown at 30 C in 15 media which produced generation times of between 200 and 40 min. A correlation was observed between the growth rate and the number of septa per cell. The time between the production of a septum and its involvement in cell division was not related to the growth rate, but, for the 15 populations, had a mean value of 138 min. Multiseptate cells became progressively evident when the organism was grown at rates in excess of a 138-min generation time.


Release of alkaline phosphatase from cells of Pseudomonas aeruginosa by manipulation of cation concentration and of pH. Pseudomonas aeruginosa ATCC 9027 contains an inducible alkaline phosphatase. The enzyme is readily removed from 14-hr cells by washes in 0.2 m MgCl(2), pH 8.4. Similar washes in tris(hydroxymethyl)aminomethane buffer, 20% sucrose, monovalent ions, or water partially release enzyme from the cells. The release of alkaline phosphatase is correlated with an increased release of protein and retention of internal enzymes. The effect of 0.2 m MgCl(2) washing upon the cells is minimal since both viability and growth rates remain unchanged as compared to water washing. Although cells are plasmolyzed in both 0.2 m MgCl(2) and 20% sucrose, it is evident that plasmolysis alone is unable to account for total enzyme release and that a divalent metal, i.e. Mg(2+), augments the release pattern. Growing cells in the presence of increasing concentrations of MgCl(2) or at increased pH values results in an almost total secretion of the enzyme to the culture filtrate. The findings suggest that P. aeruginosa alkaline phosphatase is linked to the exocytoplasmic region through divalent metal ion, presumably Mg(2+), bridges.


Organization of polyaromatic biosynthetic enzymes in a variety of photosynthetic organisms. Sucrose density gradient centrifugation was used to estimate the molecular weights and determine possible physical aggregation of the enzymes catalyzing steps 2 to 6 in pre-chorismic acid polyaromatic biosynthesis in Anabaena variabilis, Chlamydomonas reinhardi, Euglena gracilis, Nicotiana tabacum, and Physcomitrella patens. In A. variabilis, the five enzymes are separable. Similar results were obtained for P. patens, N. tabacum, and C. reinhardi extracts, except that dehydroshikimate reductase and dehydroquinase were not separable by this method. Evidence is presented for an enzyme aggregate containing five activities, with a molecular weight of approximately 120,000 in E. gracilis; dissociation of this aggregate into components corresponding to molecular weight of ca. 60,000 is also observed. Preliminary evidence concerning the enzymatic composition of the 60,000-molecular-weight components is presented and discussed. Similarities between the E. gracilis polyaromatic aggregate and that of Neurospora crassa are discussed.


Methylated bases of Bacillus megaterium KM nucleic acids: comparison with Escherichia coli. Parallel studies were performed with methionineless derivatives of Escherichia coli 15 T(-) and Bacillus megaterium KM: T(-). Methylated bases are present in the total cell ribonucleic acid (RNA) of B. megaterium. The level of RNA methylation in E. coli is about 60% greater than that in B. megaterium. Although E. coli deoxyribonucleic acid (DNA) was found to contain 0.12% 5-methylcytosine (5-MC) and 0.24% 6-methylaminopurine (6-MA), methylated bases were not detected in the DNA of B. megaterium. Assuming a molecular weight of 7 x 10(9) daltons for B. megaterium DNA, it was calculated that this organism could not contain more than one molecule of 5-MC or 6-MA per genome, and that possibly no methylated bases were present. Methylated bases were also not detected in the DNA of thymine-starved B. megaterium. Crude extracts of this organism possess RNA methylase activity but no detectable DNA methylase activity.


Isolation and preliminary characterization of developmental mutants from Microsporum gypseum. Developmental mutants affected in either sporulation or spore germination have been isolated from Microsporum gypseum with the aid of nitrosoguanidine or as spontaneously occurring mutants. The time course levels of several proteins temporally associated with conidial development have been assayed in the wild-type and mutant strains. The spore germination characteristics of two of the mutants are described. The relationship of alkaline protease accumulation to tyrosinase accumulation and spore germination is discussed.


Citric acid cycle: gene-enzyme relationships in Bacillus subtilis. The genetic location of mutations affecting the citric acid cycle and the properties of mutants of Bacillus subtilis possessing these mutations have been examined. Genes coding for the component enzymes of the cycle were found to be unlinked to each other and thus do not form an operon. The mutational defect in a mutant lacking fumarase mapped between thr-5 and cysB3. Mutations causing inability to produce isocitrate dehydrogenase and succinate dehydrogenase were found to map between argA11 and leu-1. The alpha-ketoglutarate dehydrogenase mutations were mapped at the terminal end of the B. subtilis chromosome through a weak linkage in phage PBS-1 transduction of one class of these mutations of ilvA2 and metB4. A second class of alpha-ketoglutarate dehydrogenase mutations mapped closer to ilvA2 and metB4 but still terminal with respect to these markers. Aconitaseless mutants possessed mutations that could not be linked to any of the known transducing segments of the chromosome. An effect of mutation conferring loss of one enzyme of the cycle on the specific activity of the other enzymes in the cycle was observed.


Effect of prostaglandin E1 on clot retraction.


Association of the Bacillus subtilis chromosome with the cell membrane: resolution of free and bound deoxyribonucleic acid on renografin gradients. Linear density gradients of Renografin have resolved two components of bacterial deoxyribonucleic acid (DNA) in sheared lysates. Component 1, at equilibrium density after 5 hr of centrifugation, is enriched for newly synthesized DNA and markers near the origin and terminus of replication. It contains 5% of total cellular protein, 25% of the phospholipids, 30 to 50% of the DNA, 4 to 11% of unstable ribonucleic acid (RNA), RNA polymerase, and low amounts of DNA polymerase. The material is sensitive to Pronase and Sarkosyl. In unsheared lysates, all of the DNA forms a band at this position. Shearing the lysate generates a slow-sedimenting fraction of DNA (component 2) which contains more uniformly labeled than newly synthesized DNA. These observations suggest that replicating DNA and DNA at the origin and possibly the terminus of replication are associated with membrane. The amount of uniformly labeled DNA in component 1 and an estimate of the number of chromosomal fragments suggest that other parts of the chromosome are possibly associated with the membrane.


Reversible mechanochemical cycle in the contraction of Vorticella.


Demonstration and implications of lysozyme and immunoglobulins in human ear wax.


Tapeworm cyst infestation of the brain.

Tapeworm cyst infestation of the brain.


The hemodynamic consequences of moderate postoperative anemia in monkeys.


Control of alloxan diabetes in dogs by islet cell transplantation.


Ureteral ectopia into a Wolffian duct remnant (Gartner's ducts or cysts) presenting as a urethral diverticulum in two girls.


An unusual bone dysplasia: parastremmatic dwarfism.


External small intestinal fistulas.

External small intestinal fistulas.


The surgical significance of hyperosmolar coma.


A polymeric form of haptoglobin in the gamma-irradiated Spanish goat.


Isotope exchange at equilibrium as a test for homotropic cooperativity of allosteric enzymes.


Heterogenetic antigen of Staphylococci and of some aerobic bacilli.


Kinetics studies on the fluorescence quencher in isolated chloroplasts. The chlorophyll fluorescence yield in isolated chloroplasts without an added electron acceptor is increased by actinic illumination. The decline in the fluorescence yield when the actinic illumination is extinguished can be accurately represented by three, independent, exponential decays with half-times of approximately 0.8, 5, and 30 sec. These results have been interpreted using Duysens' theory of fluorescence quenching by a compound (Q) on the reducing side of photosystem II. This theory states that changes in fluorescence yield are indicative of electron flow through Q. The most rapid decay is eliminated by an EDTA washing of the chloroplasts and the half-time is increased by uncoupling with ammonia and by added electron acceptors in suboptimal concentrations. Thus, this decay may represent electron flow from Q to intermediates on the oxidizing side of photosystem I. The decay with a half-time of 5 sec is affected in the same manner as the decay with the shortest half-time by the same procedures. However, electron donors to photosystem II lengthen the half-time of the 5 sec decay while eliminating the most rapid decay. This 5 sec decay can be interpreted as electron flow from Q to intermediates either on the reducing side of photosystem II or on the oxidizing side of photosystem I. The decay with the longest half-time is affected only by pH and electron donors to photosystem II. Therefore, this decay may indicate electron flow from Q to intermediates on the oxidizing side of photosystem II which may be connected to the regeneration of the oxygen burst.


Determination of pancreatic amylase activity by a radial diffusion assay.


The surgical dilemma in the primary therapy of invasive breast cancer: a critical appraisal.


The nigerian neurological profile.

The nigerian neurological profile.


Phenolase of papaver somniferum. I. Isolation of the enzyme and its substrate specificity.


Activation of rabbit muscle phosphoglucomutase by acid and alkali.


Evidence for an absence of myoglobin from human smooth muscle.


Phosphoglucomutase, carbonic anhydrase and catalase in Indonesians.


Radio-sensitization with extra-cellular halogenated purines and pyrimidines and related compounds.


Intracerebral temperature changes after x-irradiation in the rabbit.


Enhancement of viral transformation by ultra-violet light.


Total parenteral alimentation solution.

Total parenteral alimentation solution.


Altered reflex vasodilatation in the hypertensive rat: possible role of histamine.


Cerebellar hypoplasia in neonatal rats caused by lymphocytic choriomeningitis virus. Lymphocytic choriomeningitis virus, strain E-350, when inoculated intracerebrally in rats 1 to 7 days old, produces an acute destructive infection of the cerebellar cortex resulting in permanent cerebellar hypoplasia and ataxia. Several other arenoviruses may produce a similar lesion in neonatal rodents.


Eression of allelic immunoglobulin in homozygous rabbits injected with RNA extract. Ribonucleic acid extracts obtained from lymph nodes of immunized rabbits homozygous for the b(4)or b(5)allele of light chain immunoglobulin allotypes were injected intravenously into nonimmunized rabbits homozygous for the alternate allele. Approximately 30 percent of the plaque-forming cells in the spleen yielded plaques with immunoglobulin M antibody possessing the allotype of the RNA donor. The allotype of the RNA donor was also found in the IgG immunoglobulin of lymphoid cell lysates as well as in the IgG isolated from the serum. These results suggest that the injected RNA has an informational role in the in vivo synthesis of immunoglobulins by host lymphoid cells.


Distribution of transferrin phenotypes in selected troops of Kenya baboons.


The Ap-Bp blood groups of baboons.

The Ap-Bp blood groups of baboons.


Laryngeal edema. An experimental study.

Laryngeal edema. An experimental study.


Discharged mental patients--are they really in the community?


Pulp protection requirements for two composite resin restorative materials.


Macroglobulinemia with positive antiglobulin tests.


Role of tricarboxylic acid cycle in bacterial sporulation.


35Cl-NMR studies of Co2+ carbonic anhydrases.


Turnover of the cell wall of Bacillus subtilis W-23 during logarithmic growth.


Methylation of DNA in vivo by thymus, spleen, and liver of the rat.


Further studies on the ribonuclease inhibitor from rat liver: stability and other properties.


An anatomical study of converging sensory pathways within the cerebral cortex of the monkey.


Thalamic neurons responsive to temperature changes of glabrous hand and foot skin in squirrel monkey.


Corticospinal projections from the pre- and postcentral gyri in the squirrel monkey (Saimiri sciureus).


Local taste specificity within lingual nerve fields in the cat.


Disruption of DNA synthesis in Euplotes by heat shock.


Sensitization to the enzymes in detergents.


Chronic and acute toxicity of the chlorinated hydrocarbon insecticides in mammals and birds.


Bromine catalysis for carbon dioxide hydration and dehydration and some observations concerning the mechanism of carbonic anhydrase.


Specific granulomatous hypersensitivity elicited by bentonite particles coated with soluble antigens from schistosome eggs and turcle bacilli.


Abortion sequel--Rh problems.

Abortion sequel--Rh problems.


Inoculation of cariogenic streptococcus (FA-1) and other bacteia i the pulp in rats. Topical pathological chanes and general dissimination.


ALG treatment of Rhesus monkeys. Attempts to reduce complications by the induction of tolerance.


Improved complementation in the cytotoxic test.


The fine structure of babesia microti in the natural host, microtus agrestis.


Auxotrophic mutation associated with low streptomycin resistance and slow growth in Bacillus subtilis.


Fast sedimenting fractions containing DNA from Streptomyces griseus.


Serological and chemical studies of Sh. sonnei, Pseudomonas shigelloides and C27 strains.


Immunological and biochemical activity of anti-ribosome immune serum.


Hepatic amebiasis.

Hepatic amebiasis.


Epikeratoprosthesis in rhesus monkeys.

Epikeratoprosthesis in rhesus monkeys.


Animal reference virus recommendations.

Animal reference virus recommendations.


Serologic differentiation between African horse-sickness and equine arteritis.


Etiologic studies of transmissible gastroenteritis of swine.


Purification of two plaque variants of sindbis virus.


Nucleic acid synthesis during the focus formation by Shope fibroma virus on green monkey kidney cells.


Transmissible gastroenteritis of swine: virus-intestinal cell interactions. I. Immunofluorescence, histopathology and virus production in the small intestine through the course of infection.


Serological behaviour of isolated African swine fever virus. Brief report.


Transmissible gastroenteritis of swine: virus-intestinal cell interactions. II. Electron microscopy of the epithelium in isolated jejunal loops.


Fibrin degradation products in thrombotic thrombocytopenic purpura.


Complement-fixing antibodies to rickettsial antigens in sera of primates.


Susceptibility studies of gram-negative bacilli against carbenicillin.


Immunological studies in experimental cancer: Yoshida ascites sarcoma in rats.


Plasma histaminase in patients of bronchial asthma and the effect of prednisolone administration on it.


Large doses of nicotinic acid therapy in frostbite in monkeys.


Serological diagnosis of parasitic diseases.


Somatotopic organization of spider monkey somatic sensory cerebral cortex.


Pathological analysis of intracranial space-occupying lesions in 1000 cases including children. 3. Vascular tumours and hamartomas; meningiomas; schwannomas.


Conjoined twins.

Conjoined twins.


Relationship of the birth canal to the bacterial flora of the neonatal respiratory tract and skin.


The experimental subject--a choice, not an echo.


Obstructive uropathy in infants.

Obstructive uropathy in infants.


Teratogenic effect of rubella virus.

Teratogenic effect of rubella virus.


Radioactive estradiol accumulation in endometriosis of the rhesus monkey.


The cancer mortality. Review concept.

The cancer mortality. Review concept.


Principles and methods of obscurantism.

Principles and methods of obscurantism.


Unproven methods of cancer treatment.

Unproven methods of cancer treatment.


A survey of the attitudes of second, third and fourth year medical students toward the "curability" of breast cancer.


Oncology in medicine.

Oncology in medicine.


Immunologic evidence for monkey and human prolactin (MPr and HPr).


Placental content and characterization of human chorionic thyrotropin.


Thermal stability of serum thyroxine-binding proteins.


Speed, accuracy, and strength of forelimb movement after unilateral pyramidotomy in rhesus monkeys.


Heat resistance of certain pathogenic bacteria in milk using a commercial plate heat exchanger.


Effects of anabolic steroids on hormone-binding proteins, serum cortisol and serum nonprotein-bound cortisol.


Disseminated endometriosis in a Rhesus monkey (Macaca mulatta). Histogenesis and possible relationship to irradiation exposure 1,2.


Malignant abdominal tumors in children.

Malignant abdominal tumors in children.


Growth of selected bacteria in liquid media containing DDT, dieldrin and heptachlor.


Cell differentiation and the development of colonic neoplasms.


Further characterization of TRIC agent strains of genital origin.


Organized medicine and the delivery of medical care in the seventies.


Effect of local intraarterial administration of bradykinin and hydergine in obstructive arterial disease.


Reversal of hyperventilation induced hyperlactatemia by acetazolamide.


Cholesterol synthesis by the gastrointestinal tract.


The regulation of aconitase and isocitrate dehydrogenase in sporulation mutants of Bacillus subtilis.


Morphometric estimation of pulmonary diffusion capacity. I. Model and method.


Effects of magnesium deficiency on ribosomal structure and function in certain Gram-positive and Gram-negative bacteria.


Commitment to sporulation and induction of glucose-phosphoenolpyruvate-transferase.


The mechanism of acquired resistance to Co2+ and Ni2+ in Gram-positive and Gram-negative bacteria.


Interrelationships between the utilization of magnesium and the uptake of other bivalent cations by bacteria.


An analysis of vacuum effects in the sterilization of microorganisms.


Phosphorylase alpha activity in rat uterine homogenates. Loss of activity related to in vivo treatment with estrogen, progesterone, relaxin and CaEDTA.


Diabetes and menstruation.

Diabetes and menstruation.


Activity of some agents in the lysogenic induction in Corynebacterium diphtheriae strain No. 25.


Cirrhosis associated with the Australia antigen in an infant who acquired hepatitis from her mother. A 19-week-old English girl developed acute viral hepatitis, which became chronic with persistent hepatosplenomegaly and abnormal liver function tests. Liver biopsy at 1 year showed an active cirrhosis with multinucleated giant cells. The Australia (Au) antigen was detected repeatedly in the infant's serum by immunodiffusion and by electron microscopy at the time of the acute attack and during the development of cirrhosis. She had apparently acquired the hepatitis from her mother, who had had jaundice at the end of pregnancy and for one month thereafter, and who was subsequently shown to be a carrier of Au antigen. Particles with surface projections resembling paramyxoviruses were observed in two of the later specimens of the infant's serum.


Photoreactivation and photoprotection of lysogenic induction in Corynebacterium diphtheriae strain No. 25.


Aflatoxin B1 effect on enzyme biosynthesis in Bacillus cereus and Bacillus licheniformis.


Chemical composition of lipopolysaccharide from an avian strain of Escherichia coli O18.


Physiology of menstruation.

Physiology of menstruation.


Study of RNA in nerve cells.

Study of RNA in nerve cells.


Proinsulin.

Proinsulin.


Antibody-neutralized avian infectious bronchitis virus in chicken embryo kidney cells: entry and degradation.


Histochemical studies on phosphorylase and amylo 1,4 lead to 1,6-transglucosidase activity in the liver. II. The relationship of glycogen structure to diffusion during histochemical incubation.


Histochemical studies on phosphorylase and amylo 1,4 lead to 1,6-transglucosidase activity in the liver. I. Species related diffusion rate of glycogen during histochemical incubation.


Unexpected serological results in haemolytic disease of the newborn.


Comparative aspects of lipogenesis in mammalian tissues.


Summary of recent abstracts. IX. Helminthiasis. Trematode infections.


Comparative behavior of human and animal tissue during experimental tooth movement.


The factor-X defect: recognition of abnormal forms of factor X.


Community participation for equity and excellence in health care. General discussion I.


Can we avoid a 100 billion dollars misunderstanding?


Comparative effects of flurazepam hydrochloride (dalmane) and placebo in patients with insomnia.


Clinical trial with sulphamethoxazole--trimethoprim in infantile gastroenteritis.


Oral contraception with estrogen and progestin combinations.


Study of molindone in disturbed preschool children.


Clinical experience with pimozide.

Clinical experience with pimozide.


Treatment of anxious depressive patients in general medical practice.


Treatment of depression with dexedrine and demerol.


Open trial evaluation of keto-imipramine.


Doxepin as adjuvant medication in the treatment of chronic schizophrenic patients: a comparative study.


BC 105 in the prophylaxis of migraine.

BC 105 in the prophylaxis of migraine.


Propranolol in the treatment of disturbances of the autonomic nervous system.


Tolazamide in the treatment of diabetes mellitus: clinical experience and review of the literature.


The identity of Streptococcus faecalis ATCC 9790.


The decarboxylation of L-lysine by Pseudomonas aeruginosa isolates.


Tuberculous morbidity in Swiss albino mice immunized with BCG. Study was made of the effect of BCG vaccination on the establishment of experimental tuberculous infection in mice. To this end, mice were infected with small quantities of virulent human bacilli administered by the respiratory route. The quantity of bacilli present for inhalation was so controlled as to permit only a finite percentage of unvaccinated animals to become infected. The relative proportion of mice infected by inhaling these small numbers of virulent bacilli was not found to be lowered by prior BCG vaccination. Thus, pulmonary tissue of immunized mice was not able to prevent the initial lodgement of virulent tubercle bacilli. The growth of virulent organisms in infected lungs, however, was significantly retarded by prior vaccination.


Differentiation of the serologicl response to Yersinia enterocolitica and Brucella abortus in cattle. The serological responses of cattle to inoculation with Brucella abortus and Yersinia enterocolitica type IX were compared. Complete cross-reactions were found in serum agglutination, antiglobulin, complement fixation and Rose Bengal plate tests. The cross-reaction between Br. abortus and Y. enterocolitica IX was confirmed by immunodiffusion tests. Although antibodies specific for each organism could also be detected by immunodiffusion tests with high titre rabbit or bovine sera, these tests were insufficiently sensitive for routine diagnostic use.A quantitative Rose Bengal plate test, using Rose Bengal stained Br. abortus and Y. enterocolitica IX, was developed which enabled the antibody responses to the two organisms to be differentiated. The specificity of this test was confirmed by cross-absorption experiments and its sensitivity was sufficient to permit evaluation of all bovine sera giving positive reactions to the serum agglutination test.


Parenteral and oral alimentation in the treatment of the nonspecific protracted diarrheal syndrome of infancy.


Total parenteral nutrition.

Total parenteral nutrition.


The complement system in chronic glomerulonephritis: three newly associated aberrations.


Disseminated intravascular coagulation in a neonate treated with heparin.


Fractionation of human spermagglutinating sera.


Roentgenology of Reiter's syndrome.

Roentgenology of Reiter's syndrome.


Production of fetal asphyxia in the rhesus monkey by administration of catecholamines to the mother.


Mermet (MER) strain. AV-782.

Mermet (MER) strain. AV-782.


Congo (CON) strain. 3011.

Congo (CON) strain. 3011.


Kamese (KAM) strain. MP 6186.

Kamese (KAM) strain. MP 6186.


The antimalarial activity of some quinolone esters.


Inflammatory changes in small blood vessels induced by kallikrein (kininogenase) in the blood of Macaca mulatta infected with Plasmodium knowlesi.


Pathological lesions produced in the brain by kllikrein (kininogenase) in Macaca mulatta infected with Plasmodium knowlesi.


A case of bicarbonate-losing renal tubular acidosis with defective carboanhydrase activity.


Mesial drift of teeth in adult monkeys (Macaca irus) when forces from the cheeks and tongue had been eliminated.


The pharmacology of fluspirilene (R 6218), a potent, long-acting and injectable neuroleptic drug.


Pharmacological studies of a new anti-inflammatory drug, 1-phenylsulfonyl-5,5-diphenylhydantoin (PC-796) in experimental animals. 3. Absorption, tissue distribution and metabolic fate.


The management of urethral diverticulum in the female.


The significance of kinin-destroying enzymes activity in Plasmodium knowlesi malarial infection.


Lactic acidosis.

Lactic acidosis.


Hepatitis-associated antigen in liver disease in Kenya.


Heterotopic bone formation in the lung.

Heterotopic bone formation in the lung.


A male sex-attractant pheromone in rhesus monkey vaginal secretions.


Patterns of distribution and urinary excretion of male rat accessory sex gland-specific antigens.


Effect of unilateral ovariectomy on twinning frequency in the marmoset.


Induction of ovulation in Macaca irus with clomiphene citrate and gonadotrophic hormones.


Dysentery produced by oral administration of the colonic contents of cynomolgus monkeys infected with Shigella flexneri 2a.


Some physiological mechanisms of diphtheria toxin production.


Effect of attrition on subjacent dentin and pulp.


Studies on the serum therapy of type E botulism: absorption of toxin from the gastrointestinal tract.


Molecular configuration and toxicity of cord factor.


Studies on atypical mycobacteria. VII. The transformation of the color of atypical mycobacteria.


Contamination of simian viruses in African green monkeys (Cercopithecus aethiops).


Mammalian scent marking.

Mammalian scent marking. Mammals mark frequently in any situation where they are both intolerant of and dominant to other members of the same species. In other words, they mark when they are likely to attack another member of the same species, and are likely to win if they do attack. Such a situation occurs, as Hediger (13) pointed out, in connection with territoriality but it also occurs in other kinds of social systems. Frequent, vigorous marking occurs at times when there is reason to infer that the animal is motivated to aggression. The effects of marks and marking upon other individuals are poorly understood. Many species mark with more than one source of scent in response to one stimulus or set of stimuli.


Prostaglandins.

Prostaglandins.


Chylous ascites in children.

Chylous ascites in children.


Early plasma protein recovery after total volume plasmapheresis in baboons.


Anti-bacterial activity of ungulic acid.


Activity-structure relationships of the thyrotropin releasing hormone.


Incorporation of branched-chain C6-fatty acid isomers into the related long-chain fatty acids by growing cells of Bacillus subtilis.


The problems of anticoagulant control. 1. Drug interactions.


Chloroplast aging in vitro and relationships to fatty acids and polyphenoloxidase activity.


Antigenic components of normal and burned mouse skin.


The effect of diet on bile volume and total bile salt secretion in rhesus monkeys.


Inheritance, hormonal regulation and properties of polymorphic murine antigens Mud1 and Mud2.


Inhibition of reagin-induced PCA in monkeys by injection of heterologous serum.


The response in mice to heat treated scrapie agent.


Initiation and differentiation of microtubule patterns in the ciliate Nassula.


Intracellular distribution of ribosomes and polyribosomes in Bacillus megaterium.


The role of exonuclease and beta protein of bacteriophage lambda in genetic recombination. I. Effects of red mutants on protein structure.


Eye dominance in a monkey.

Eye dominance in a monkey.


Reversible change in iodine sensitivity of penicillinase by alkali treatment.


The influence of concentrated electrolytes on the constitutive penicillinase synthesis of B. cereus (short communication).


Pathodynamics of blunt chest trauma: a preliminary report.


Vaccine prophylaxis for pseudomonas infections.


Interstitial pneumonitis due to hypersensitivity to an organism contaminating a heating system.


Investigation of the penicillinase activity in L colonies of Staphylococcus aureus. Penicillinase-producing strains of Staphylococcus aureus are transformed into stable L colonies by 70 to 100 subcultures on methicillin-containing medium with a suitable high osmolarity. During transformation, the penicillinase activity is lost. This loss in activity is not the result of only the penicillinase-negative mutants transforming to L colonies. If unstable L colonies are filtered through 0.45-mum membrane filters immediately after transformation, still no penicillinase activity is seen; this is also the case if the filtrated L colonies are reverted into coccal forms. The mechanism responsible for the loss of penicillinase activity is discussed. A loss of the penicillinase plasmid is proposed as the most reasonable explanation.


Effects of atmospheric humidity and temperature on the survival of airborne Flavobacterium. The survival of airborne Flavobacterium sp. in particle sizes ranging from 1 to 5 mum was significantly influenced by atmospheric temperature. A progressive increase in temperature from -18 to 49 C resulted in increases in death rates of the airborne organism. The lowest death rates were observed in the temperature range of -40 to -18 C, and the highest death rates were observed in the 29 to 49 C range. At 24 C, the survival of airborne Flavobacterium did not appear to be significantly affected by relative humidity ranging from 25 to 99%.


Susceptibility of Neisseria meningitidis strains from the civilian population to sulfadiazine, penicillin, and rifampin. The minimal inhibitory concentration (MIC) values of sulfadiazine, penicillin, and rifampin for meningococcal strains isolated from civilians during 1970 were compared. The strains were isolated from various sources and geographical areas and represented several serogroups. The ranges of MIC values were as follows: 0.05 to 20 mg/100 ml for sulfadiazine, 0.01 to 0.4 mug/ml for penicillin, and 0.01 to 0.8 mug/ml for rifampin. There was no significant relationship between MIC values of sensitive or resistant sulfadiazine strains and the MIC values to the other two antimicrobial agents. Comparisons of sulfadiazine MIC values with inhibition zones around sulfathiazole discs showed excellent correlation, provided the strains were separated into sensitive and resistant groups on the basis of growth at 1 mg/100 ml. Regression curves for penicillin and rifampin sensitivity showed homologous sensitive populations with the strains studied.


Standardization of human diploid cell cultivation. Human embryonic diploid lung fibroblasts grown in Eagle's medium were exposed continually to a variety of environmental conditions over a large number of passages to observe how these conditions affected the growth and longevity of these cells in vitro. The cells grew well at temperatures between 34 and 37 C and some cells could be adapted to grow at 40 C. Very limited growth occurred at 30 to 31 C; however, confluent monolayers of cells could be maintained for months at 30 C and still give rise to actively growing cultures. Increasing the amino acid concentration in Eagle's medium or the calf serum concentration above 10% had no effect on the growth rate or longevity. One per cent calf serum could not support prolonged active growth. Trypsin concentrations between 1 and 0.1% and crystalline trypsin at 50 mug/ml showed no influence on cell growth. Ethylenediaminetetraacetic acid treatment and scraping, however, destroyed many of the cells, and the survivors grew poorly. The clonal morphology varied with age. Young cells frequently gave rise to densely packed clones, whereas older cells gave rise to clones with widely scattered cells. The cloning efficiency was high when the cells were young but decreased rapidly with successive passage. It was relatively constant from the 7th to 20th passage at about 15%.


Reactivity of two gonococcal antigens in an automated microhemagglutination procedure. In studies of several hundred sera, a passive-hemagglutination technique with soluble antigen of sonically treated gonococci as the sensitizing material for tanned erythrocytes and Neisseria sicca sonically treated material as an absorbent detected gonococcal antibodies in 77% of males and 88% of females infected with uncomplicated gonorrhea. However, 6% of the sera from individuals in celibate religious orders and 18% of the sera from a group of females having cervical cultures negative for gonococci were also reactive with this procedure. Erythrocytes sensitized with an alkaline extract of gonococci reacted with 23% of the sera from infected males, 49% of the sera from infected females, and 2% of the sera from celibate females.


Experimental technique for studying aerosols of lyophilized bacteria. An experimental technique is presented for studying aerosols generated from lyophilized bacteria by using Escherichia coli B, Bacillus subtilis var. niger, Enterobacter aerogenes, and Pasteurella tularensis. An aerosol generator capable of creating fine particle aerosols of small quantities (10 mg) of lyophilized powder under controlled conditions of exposure to the atmosphere is described. The physical properties of the aerosols are investigated as to the distribution of number of aerosol particles with particle size as well as to the distribution of number of bacteria with particle size. Biologically unstable vegetative cells were quantitated physically by using (14)C and Europium chelate stain as tracers, whereas the stable heat-shocked B. subtilis spores were assayed biologically. The physical persistence of the lyophilized B. subtilis aerosol is investigated as a function of size of spore-containing particles. The experimental result that physical persistence of the aerosol in a closed aerosol chamber increases as particle size is decreased is satisfactorily explained on the bases of electrostatic, gravitational, inertial, and diffusion forces operating to remove particles from the particular aerosol system. The net effect of these various forces is to provide, after a short time interval in the system (about 2 min), an aerosol of fine particles with enhanced physical stability. The dependence of physical stability of the aerosol on the species of organism and the nature of the suspending medium for lyophilization is indicated. Also, limitations and general applicability of both the technique and results are discussed.


Neutron activation analysis of manganese and sodium in bacterial cells. The application of neutron activation analysis for mineral determinations in bacteria was investigated. Elements considered here were manganese and sodium. The sporeformer Bacillus megaterium ATCC 19213 was utilized. With this method, the manganese and sodium levels of whole and ashed vegetative cells, sporulating cells, and free spores were determined. The culture medium was also analyzed for these two elements. The results indicate that neutron activation analysis is readily applicable to the study of mineral content of bacterial cells, spores, and culture media. The method has been shown to be ideal for the study of incorporation and egression of mineral elements during vegetative growth and secondary metabolism of sporulation.


Technique for viral neutralization antibody surveys in primary microcultures. A simplified, microplate tissue culture procedure is described which facilitates neutralization antibody surveys on large numbers of sera. A comparison of the micromethod with that employing standard tube cultures indicates it to be as sensitive and far more economical.


Antibacterial activity of gentamicin sulfate in tissue culture. Gentamicin is a more effective in vitro bacterial inhibitor than combined penicillinstreptomycin, is nontoxic to tissue culture monolayers, and does not inhibit virus replication.


Prevention of haemolytic disease of the newborn.


The ultrastructure of white blood cells and thrombocytes of the domestic fowl.


Effect of chloramphenicol and actinomycin D on lysogenic induction and vegetative development of phages in Corynebacterium diphtheriae Strain No. 25.


Isolation and overall characterization of the "inner matrix" from mitochondria of primate liver cells. II. A combined ultrastructural and quantitative evuation of the high pressure-accessible coartment.


Percutaneous heart catheterization in infants and children. II. Prospective study of results and complications in 127 consecutive cases.


The hydrational effect of alkali metal and halide ions on the Rh-anti-Rh system.


The protection of Streptococcus faecalis with gamma-irradiation against the bactericidal effect of potassium permanganate.


The cytotoxic effect of heterologous antisera to mouse IgG on mouse lymphocytes.


Cyclization of eucaryotic deoxyribonucleic acid fragments.


A procedure for gene purification: the purification of the ribosomal RNA genes of Bacillus subtilis as DNA-RNA hybrids.


Ribonucleoprotein complexes containing messenger ribonucleic acid from rat liver polyribosomes: analysis of conditions of their appearance after diaminoethane tetra-acetic acid treatment of polyribosomes.


Interpretation of serum tri-iodothyronine levels measured by the sterling technic.


Intrauterine pregnancy and coexistent pelvic inflammatory disease.


Influence of pretreatment of the redoxpotential electrode on the determination of oxygen pressure in bacterial cultures.


Efficiency of medium B-2 for the isolation of tubercle bacteria from menstrual blood and from endometrium in female genital tuberculosis.


A 2-year study of the efficacy and tolerability of prenylamine in the treatment of angina pectoris.


The chemistry of guanidines and their actions at adrenergic nerve endings.


Collagen metabolism of skin and periodontal membrane in the squirrel monkey.


Effect of phenobarbital and SKF 525-A on the teratogenicity of cyclophosphamide in mice.


The histology of palatal closure in the rhesus monkey (Macaca mulatta).


The caseinase activity of some vermivorous cone shell venoms.


A quick method for differentiating between virulent bovine and attenuated BCG bacilli.


Some chemical properties of the venom of the scorpionfish Scorpaena guttata.


The significance of tuberculin tests in the horse.


The basis for chymotrypsin-induced glaucoma.


The extraction of protein matrix from human dental plaque.


Stimulation of lymphocyte transformation by bacterial antigens in patients with periodontal disease.


The precursors of nicotinic acid in bacteria.


Enzymic reduction of the C-terminal dehydroalanyl-lysine sequence in nisin.


Cross-linking of bacterial cell walls with glutaraldehyde.


Steatorrhoea due to duodeno-colic fistulae from perforation of a duodenal diverticulum with ectopic gastri epithelium.


A comparison of heavy water and beryllium as cyclotron targets for fast neutron production for radiotherapeutic applications.


Chromosome mapping.

Chromosome mapping.


Diabetic ketoacidosis and influenza.

Diabetic ketoacidosis and influenza.


Two populations of Rh groups together with chromosomally abnormal cell lines in the bone marrow. At the onset of his disease a man with polycythaemia vera had chromosomally normal cells in the bone marrow and Rh blood group CDe/cDE. Five years later he developed pancytopenia with erythroid hyperplasia of the bone marrow. This was associated with the presence of a major abnormal clone, 45,XY,B-,C-,16+, a minor clone, 45,XY,2+,3-,C-, and a few apparently normal cells. At the same time Rh blood grouping showed two populations of red cells, one CDe/cDE and one giving the reactions of CDe/CDe which can be interpreted as CDe. If monosomic CDe be the correct interpretation the case provides a strong hint that the Rh complex locus is sited either on the long arm of a B-group chromosome or, less probably, on an autosome of the C group.


Survey for trypanosome infections in domestic cattle and wild animals in areas of East Africa. II. Salivarian trypanosome infections in wild animals in Busoga District, Uganda.


Physiological roles of A and C fiber inputs to the spinal dorsal horn of Macaca mulatta.


Changes in evoked potentials after experimental cervical spinal cord injury in the monkey.


Cerebrospinal fluid production rates determined by simultaneous albumin and inulin perfusion.


Factors influencing osteogenesis.

Factors influencing osteogenesis.


Metabolic interlock. The multi-metabolite control of prephenate dehydratase activity in Bacillus subtilis.


Purification and properties of trypsin-like proteases from the starfish Dermasterias imbricata.


Substrate activation and the kinetics of ferroxidase.


The cerebrospinal fluid in diabetic ketoacidosis.


Multiple cell origin of hereditary neurofibromas.


Recovery of Neisseria gonorrhoeae from "Sterile" synovial fluid in gonococcal arthritis.


Ketones and coma.

Ketones and coma.


Autoallergy in burns: an attempt to demonstrate burn-specific antigen in experimental thermal trauma.


Hemolymph test. A technique for detection of rickettsiae in ticks.


An in vitro system for screening potential antimalarial drugs.


Immunosuppression in experimental acute and subacute Chagasic myocarditis.


Recrudescence of acute photosensitivity following short-term steroid therapy.


The probable pathogenic role of adenosine in malaria.


Chemical versus spectrophotometric determination of bilirubin in amniotic fluid, and the influence of hemoglobin and metheme pigments.


Quantitation of sputum protein by use of the biuret reaction.


Anti-K2(Cellano) blood group antibodies. Typing as IgG and IgA with a review of their clinical significance.


Increased urinary dihydroxyphenylalanine oxidase activity in patients suffering from malignant melanoma.


The proteins of human gallbladder bile with and without gallstones.


Lipid changes in the plasma lipoproteins of baboons given an atherogenic diet. 2. Changes in the phospholipid classes of total plasma and of alpha- and beta-lipoproteins.


Hemopexin in cord blood.

Hemopexin in cord blood.


Lateral preferences in the monkey.

Lateral preferences in the monkey.


Visual control of limb movement following section of optic chiasm and corpus callosum in the monkey.


An immunological time scale for primate evolution consistent with fossil evidence.


Immune precipitation of purified chicken antibody at low pH.


Differential operant conditioning of heart rate in the restrained monkey.


Differential effects of amphetamine and scopolamine on matching performance of monkeys with lateral frontal lesions.


Some bio-electric parameters of early Xenopus embryos.


A comparison of various measures of sensitivity of M. tubercolosis to pyrazinamide.


Observations in vitro on the suitability of pyrazinamide for intermittent chemotherapy of tuberculosis.


Broncho-pulmonary lesions associated with tuberculous lymph node compression--'node compression syndrome'.


On the role of culture medium asparagine in tuberculin production.


Mycobacterium avium infections in cattle and pigs in Great Britain.


The status of zinc in human nutrition.

The status of zinc in human nutrition.


The effect of nitroglycerine and amyl-nitrite on central haemodynamics in patients with coronary heart disease.


Contribution on the morphology of coronary sclerosis.


The effect of prolonged bed rest on postpartal cardiomyopathy.


Steroids in amniotic fluid.

Steroids in amniotic fluid.


Studies on Asiatic influenza viruses. 8. Isolation of A2 influenza virus on surviving fragments of chick embryo chorioallantoic membranes.


Studies on gamma-glutamyl peptidase from Pseudomonas aeruginosa.


The release of radioactive nucleic acids and mucoproteins by trypsin and ethylenediaminetetra-acetate treatment of baby-hamster cells in tissue culture. Monolayers of baby-hamster kidney cells were grown on glass in tissue culture and harvested with trypsin or EDTA in order to investigate the cell surface macromolecules removed by these cell-disaggregating agents. The release of nucleic acids from the cells during the harvesting procedure was monitored by labelling the cellular RNA with [5-(3)H]uridine and the cellular DNA with [2-(14)C]thymidine. Treatment of the cells with EDTA was found to cause an increase in the permeability of the plasma membrane with 7.6% of the cellular RNA, but less than 1% of the cellular DNA, being released. Moreover, 61% of the cells harvested with EDTA were permeable to Trypan Blue. With crude trypsin, lysis of the cell occurred with the release of similar amounts of RNA and DNA amounting to about 11% of the total cellular nucleic acid. In contrast, crystalline trypsin released only 1% of the cellular nucleic acids. Since virtually all the cells (99%) after harvesting in crystalline trypsin were impermeable to Trypan Blue, this method was suitable for obtaining cell surface macromolecules without contamination by intracellular damage. [1-(14)C]Glucosamine was incorporated by the cells only into bound hexosamines and sialic acids. [By monitoring the release of radioactivity in high-molecular-weight material in such experiments a measure of the release of macromolecules containing amino sugars was obtained.] Of the total macromolecules containing amino sugars in the cells 33%, 24% and 13% were released when the cells were harvested with crude trypsin, crystalline trypsin or EDTA respectively. Crystalline trypsin also released 39% of the total sialic acid of the cell, whereas less than 1% of the cellular sialic acid was present in the EDTA-treated fraction. It is concluded that the macromolecules containing amino sugars released with crude trypsin and EDTA are likely to be heavily contaminated with intracellular material. However, the macromolecules released by crystalline trypsin appear to come from the cell surface.


Distribution of heavy chain sub-classes of immunoglobulin G in normal IgG and residual IgG from hypogamma-globulinaemic sera.


Pepsin digestion of human G-myeloma proteins of different subclasses. II. Immunochemical investigations of the products of peptic digestion.


Studies on eosinophil leucocyte migration. II. Factors specifically chemotactic for eosinophils and neutrophils generated from guinea-pig serum by antigen-antibody complexes.


Human thymus-lymphoid tissue antigen and its presence in leukaemia and lymphoma.


Experimental induction of rheumatoid factor-like substances in animal trypanosomiasis.


A reversible vascular abnormality associated with diabetic ketosis.


Evaluation of the resin strip technique for determining serum T3 binding capacity and serum thyroxine.


Affinity of IgM and IgG antibodies.

Affinity of IgM and IgG antibodies.


Physical and biological properties of a 99mTc-sulfur colloid preparation containing disodium edetate.


Choriocarcinoma scanning using radiolabeled anti-body to chorionic gonadotrophin.


Studies on cellulase of the cotton wilt pathogen Fusarium vasinfectum Atk.


Incidence rate of pulmonary aspergillosis in the High Tatra Mountains sanatoria for tuberculosis and respiratory diseases of adults.


Primary drug resistance to the major anti-tuberculosis drugs in Japan. An investigation by the Tuberculosis Research Committee (Ryoken), Japan.


Isolation of mycobacteria from tonsils, naso-pharyngeal secretions and lymph nodes in East Anglia.


Increased forearm blood flow during ketoacidosis in man.


Carbonic anhydrase isoenzymes in the erythrocytes and uterus of the rabbit. 1. Two forms of the zinc-containing enzyme carbonic anhydrase (EC 4.2.1.1) were isolated from rabbit erythrocytes and two forms from rabbit uterine tissue (endometrium) in the progestational stage of pregnancy (days 6-8 of gestation). Separation of the isoenzymes was achieved by ion-exchange chromatography, preparative polyacrylamide-gel electrophoresis and isoelectric focusing. A comparison was made of the general properties and kinetic behaviour of the purified isoenzymes. 2. Although indistinguishable in terms of molecular weight and zinc content the isoenzymes were very different as catalysts of the hydration of carbon dioxide. The two erythrocyte isoenzymes, found in almost equal amounts, differed more than 100-fold in specific activity. Of the two isoenzymes prepared from either endometrial or entire uterine homogenates one was kinetically indistinguishable from the erythrocyte high-activity form, whereas the other, also possessing high activity, was found only in the endometrial or uterine tissue. Present evidence suggests that the former isoenzyme originated from residual blood contaminating the tissue homogenates, and that a marked rise in the content of the latter isoenzyme accounts for the increase in rabbit endometrial carbonic anhydrase activity that previously has been observed in early pregnancy. 3. Minor forms of the erythrocyte isoenzymes, having a characteristic quantitative and electrophoretic relationship to one another, were occasionally produced during purification. 4. The actions were investigated of the inhibitors acetazolamide (5-acetamido-3,4-diazole-1-thia-2-sulphonamide), 1,1-dimethylaminonaphthalene-5-sulphonamide and ethoxyzolamide (6-ethoxybenzothiazole-2-sulphonamide) on the hydration of carbon dioxide and the hydrolysis of p-nitrophenyl acetate catalysed by the isoenzymes. 5. The low-activity erythrocyte isoenzyme was superior to the high-activity form as a catalyst of beta-naphthyl acetate hydrolysis.


Neurofibromatosis of the colon: a unique manifestation of von Recklinghausen's disease.


Inhibition of erythrocyte antibody binding by a dialysate of hemolyzed red blood cells.


Natural evolution and pathological alterations of lymphoid cell proteins. Immunochemical characteristics of soluble antigens.


Lipid metabolism in the fetus during development.


Extravascular albumin mass and exchange in rat tissues.


Physiology of the fetal urinary tract.

Physiology of the fetal urinary tract.


Phosphodeoxyribomutase from Escherichia coli. Purification and some properties.


Isolation of hetero antibodies to human thyroglobulin.


Evaluation of somatotropic activity using the NEFA mobilization test and radioimmunologic estimation of plasma HGH. Studies on 43 children with short stature.


Wanowrie virus: a new arbovirus isolated from Hyalomma marginatum isaaci.


The isolation of gamma-glutamyl peptides from monkey brain.


Contribution of the pentose cycle to the metabolism of glucose in the isolated, perfused brain of the monkey.


Levels of myo-inositol in normal and degenerating peripheral nerve.


Trans-sulphuration in primate brain: regional distribution of methionine-activating enzyme in the brain of the rhesus monkey at various stages of development.


Trans-sulphuration in primate brain: regional distribution of s stages of development.


Vein of Galen ligation in the primate. Angiographic, gross, and light microscopic evaluation.


Immunoallergic aspects of coccidioidomycosis.


Trace elements in intravenous fluids.

Trace elements in intravenous fluids.


Lake Erie: pollution abatement, then what?


Aflatoxin P-1: a new aflatoxin metabolite in monkeys. A new phenolic derivative of aflatoxin B(1), appearing mainly in conjugated form, was identified as the principal urinary metabolite of aflatoxin B(1) in rhesus monkeys. Its identification in human urine might facilitate estimation of aflatoxin exposure in human populations.


Coelomocyte aggregation in Cucumaria frondosa: effect of ethylenediaminetetraacetate, adenosine, and adenosine nucleotides.


Immunoglobulin G: identification of rabbit IgG in coagulo-prostatic fluid by gel diffusion precipitation & immunoelectrophoresis.


Chemical basis for the differentiation of Brucella species.


Effect of azide & glycine on heterocyst development in Anabaena ambigua Rao.


Carbenicillin in patients with cystic fibrosis: clinical pharmacology and therapeutic evaluation.


Oral or nasogastric maintenance therapy in pediatric cholera patients.


The laminar distribution of fibers of the corpus callosum: a comparative study in the rat, cat, rhesus monkey and chimpanzee.


A grip strength testing device for monkeys.


Bilateral pyramidal lesions in infant rhesus monkeys.


Relation between movements and unit activity of the basal ganglia in monkeys.


Effects of unilateral pyramidotomy on conditioned finger movement in monkey (Macaca irus).


Selective increase in choice latency after transection of a pyramidal tract in monkeys.


Investigation of social behaviour in primates (Saimiri sciureus) by means of telestimulation technique.


Acute inhalation toxicity of monomethylhydrazine vapor.


Man, chimpanzee and monkey: a neuropsychological comparison.


Prefrontal lesions in man and monkey.

Prefrontal lesions in man and monkey.


Comparison of so-called amnesic states in monkey and man.


Ipsilateral and contralateral eye-hand control in split-brain rhesus monkeys.


Burn scar carcinoma.

Burn scar carcinoma.


Cancer of the ovary.

Cancer of the ovary.


Primary management of Hodgkin's disease.


Inguinal hernia and colon carcinoma.

Inguinal hernia and colon carcinoma.


The treatment of neuroblastoma.

The treatment of neuroblastoma.


Malignant melanoma.

Malignant melanoma.


Clinical staging system for carcinoma of the oral cavity.


Differential diagnosis of malignant melanoma.


Unproven methods of cancer treatment.

Unproven methods of cancer treatment.


Carcinoma of the fallopian tube.

Carcinoma of the fallopian tube.


Report on national surveys of cytologic facilities.


Ameloblastoma.

Ameloblastoma.


Cancer of the esophagus: recent progress.


Trends in the management of cancer.

Trends in the management of cancer.


Second chance for patients with carcinoma of the cervix.


Cancer uncontrol--a nightmare.

Cancer uncontrol--a nightmare.


Gastrointestinal carcinoids and the carcinoid syndrome: clinical characteristics and therapy.


The role of the surgeon in the prospect of death from cancer.


Clinical staging system for carcinoma of the thyroid gland.


Mechnaism of action of anticancer drugs at a cellular level.


Clinical staging system for carcinoma of the urinary bladder.


Twenty-five to forty-five years of diabetes with and without vascular complications.


Soft tissue tumors: classification and principles of management.


Management of bone in the patient before, during, and after treatment for oral cancer.


Hendricks natural immunity therapy.

Hendricks natural immunity therapy.


Design and analysis of a clinical trial of antihypertensives.


Human breast mild in relationship to breast cancer.


The importance of biopsy procedures.

The importance of biopsy procedures.


A comparison of the sensitivity of electroimmunodiffusion and single radial diffusion in quantitation of immunoglobulins in dilute solution.


The paraneoplastic syndromes.

The paraneoplastic syndromes.


Prostheses, stents, and splints for the oral cancer patient.


Contractile activity of gastric antrum and taenia coli in the unanesthetized monkey.


Incidence of Brill-Zinsser disease in East Slovakia in 1956-1967.


Differential diagnosis of malignant melanoma.


Intestinal neoplasms associated with inguinal hernia.


Bio-medical detoxification therapy.

Bio-medical detoxification therapy.


The sensitivity of the human diploid cell strain WI-38 to different viruses.


Interdisciplinary approach to the treatment of cancer.


The lymphatic system in measles. The dynamics and the quantitative characteristics of cytopathic and immunomorphological changes.


Antigenic structure of tularemia microbe strains with different degrees of virulence.


Anthrax. Biological and immunological principles of diagnosis and prevention. 4. The dynamics and intensity of skin tests with anthraxin in guinea pigs inoculated with a STI vaccine.


Use of rhesus monkeys in teratological studies.


Survival of 50Cr-labeled erythrocytes as studied by instrumental activation analysis.


Shedding of discs from rod outer segments in the rhesus monkey.


The model dental patient. An empirical study of preventive health behavior.


Norrie's disease--an x-linked syndrome of retinal malformation, mental retardation and deafness.


Some links between economic status and untreated illness.


Health insurance as an income redistribution.


Dipetalonema gracile in a capuchin monkey (Cebus capucinus).


Distribution of the ABO, MNS, P, Rhesus, Lutheran, Kell, Lewis and Duffy blood groups and frequency of irregular red cell antibodies in a population of Danes aged fifty years and a population of Danes aged seventy years. From the Glostrup population studies.


Proteolytic enzymes and biological inhibitors. II. Naturally occurring inhibitors in sera from different species and their effect upon proteolytic enzymes of various origin.


Height, weight, tuberculous infection, and tuberculous disease.


Evidence of safety of long-term, high, oral doses of DDT for man.


Investigation of the kinetic characteristics of red blood cell acetylcholinesterase in ABO, Rh hemolytic disease of the newborn and thalassemia major cases.


Reticulum cell sarcoma in Waldenström's macroglobulinemia after chlorambucil treatment.


Clinical aspects of endomyocardial fibrosis in Bahia, Brazil.


New method to measure phasic coronary blood velocity in man.


Dentists, dental societies and dental review committees.


Pulp therapy for the primary dentition. II. The treatment of teeth with non-vital or degenerating pulps.


The uptake of Hg203C12 by the intestinal wall of the duodenal and leal segment in vitro.


Some factors affecting degradation of organochlorine pesticides by bacteria.


Rate of response as a visual social stimulus. In Exp. 1, a high rate of responding (chain pulling) of a stimulus monkey was established as a visual positive discriminative stimulus for the operant behavior (bar pressing) of an observer monkey. The terminal performance of the observer under conditions in which a high rate of response of the stimulus monkey alternated in a variable temporal arrangement with a zero rate of response of the stimulus monkey (negative discriminative stimulus) was essentially the same as when nonbehavioral stimuli are correlated with the availability of reinforcement. By manipulating the schedule of reinforcement to change the rate of responding of the stimulus subject without changing its rate of reinforcement, Exp. 2 showed that the effective behavioral stimulus for the observer was the rate of chain pulling by the stimulus subject. A novel intermediate rate of responding by the stimulus monkey resulted in an intermediate rate (generalization) on the part of the observer during an extinction test. These experiments demonstrated that the rate of responding of one organism can function as a discriminative stimulus to control the rate of responding of another organism; and that the rate of responding is similar to other physical stimuli in terms of discrimination and generalization.


In-vivo experiments with ethylenediamine-tetra-acetic acid and investigations into its action on penicillin-resistant Staphylococcus aureus.


Metrazol and the evaluation of seizures.


Pterygium: "thiotepa" versus beta radiation, a double-blind trial.


Summary of recent abstracts. VII. Rickettsial diseases.


Case report. Stones in the diverticulum of the female urethra.


Haemodynamic and metabolic responses to superior vena caval hyperalimentation.


Bacteriolytic enzymes from Staphylococcus aureus. Purification of an endo-beta-N-acetylglucosaminidase. On cultivation of Staphylococcus aureus in a complex liquid medium, bacteriolytic activity is found extracellularly. The maximal amount was found at the end of the exponential growth phase in batch culture, but in continuous culture run under similar conditions the yield was doubled. Isoelectric focusing of dialysed crude culture supernatants showed that the bacteriolytic activity of all four strains studied (M18, 524, Wood 46 and Duncan) was heterogeneous. The most alkaline peak of activity (isoelectric point 9.5+/-0.1) was assayed against Micrococcus lysodeikticus turbidimetrically. This bacteriolytic activity was purified more than 70-fold after continuous dialysis by adsorption on CM-Sephadex, precipitation with ethanol, heat purification, isoelectric focusing and Sephadex G-100 chromatography. The purified enzyme (isoelectric point 9.6+/-0.1) was found to give a single band on polyacrylamide-gel and cellulose acetate electrophoresis and was devoid of all 14 staphylococcal enzymes and toxins assayed for. The molecular weight is 70000+/-5000 as estimated by Sephadex G-100 and G-200 chromatography. The marked instability of the partially and highly purified enzyme was investigated. The mode of action and some properties of this enzyme are given in the following papers (Wadström & Hisatsune, 1970; Wadström, 1970). These results indicate that this extracellular enzyme which is produced by several strains of S. aureus is not a ;lysozyme' (endo-beta-N-acetylmuramidase) as previously suggested, but an endo-beta-N-acetylglucosaminidase.


The purification and properties of carbonic anhydrases from guinea-pig erythrocytes and mucosae of the gastrointestinal tract. Procedures for isolating carbonic anhydrase (EC 4.2.1.1) enzymes from the erythrocytes and the mucosae of the gastrointestinal tract of guinea pigs are described. From a haemolysate, haemoglobin was removed by the addition of ammonium sulphate, and also by two other methods, namely by gel filtration or by adsorption on DEAE-Sephadex. The crude enzyme thus obtained was resolved into the different isoenzymes by chromatography with DEAE-cellulose. From particle-free supernatants of homogenates of some gastrointestinal tissues, carbonic anhydrases were purified by ammonium sulphate fractionation, gel filtration, and ion-exchange chromatography with DEAE-cellulose. The major isoenzymes from blood, stomach, proximal colonic mucosa and caecal mucosa were homogeneous during ion-exchange chromatography, acrylamide-gel electrophoresis, and centrifugal examination. From these tissues, carbonic anhydrase was isolated as two major isoenzymes. They resemble the pairs of isoenzymes discovered in the bloods of other species. The carbon dioxide hydratase activity of one isoenzyme (;high activity' carbonic anhydrase) was 40 times that of the other isoenzyme (;low activity' carbonic anhydrase), as measured at a single substrate concentration. Two other minor components of the enzyme are also found in guinea-pig erythrocytes. All of the enzymes isolated had molecular weights of nearly 30000 (sedimentation equilibrium). ;High activity' carbonic anhydrases from blood and gastrointestinal tissues were indistinguishable according to some chemical, physical and kinetic measurements; similarly ;low activity' carbonic anhydrases from those tissues were indistinguishable. ;High activity' carbonic anhydrase was markedly different from the ;low activity' carbonic anhydrase with respect to its amino acid composition, chromatographic behaviour and isoelectric pH value. Marked differences were also found in the tissue concentrations of the major isoenzymes. It is suggested that the characteristic and selective distribution of the different forms of carbonic anhydrase in the guinea-pig tissues is related to the specific and different physiological functions of the enzymes.


Prediction of the severity of rhesus haemolytic disease by means of antibody titrations performed on the Autoanalyzer.


Effects of sulphydryl inhibitors on frog sartorius muscle: p-chloromercuribenzenesulphonic acid. 1. Experiments were done on frog sartorius muscles to study the effects and mechanisms of action of the -SH inhibitors, p-chloromercuribenzoic acid (PCMB) and p-chloromercuribenzenesulphonic acid (PCMBS).2. Both organomercurials produce a depolarization of the surface membrane which is associated with a period of asynchronous twitching and followed by inexcitability.3. Only PCMB produces a unique fractionation of the electrically evoked twitch into an initial rapid and later slow phase.4. PCMB and PCMBS increase the rate of (45)Ca efflux from whole muscle. Ethylenediamine tetraacetic acid (EDTA, 5 mM) causes only limited antagonism of the enhancement of (45)Ca efflux produced by PCMB whereas it completely antagonizes this same effect of PCMBS. EDTA selectively removes superficial calcium without penetrating into the intracellular space.5. The results suggest that PCMB inhibits -SH groups in the terminal cisternae causing a fractionation of the twitch. PCMBS acts primarily at surface sites with limited access to the cisternae and sarcoplasmic reticulum.


Investigations into the mechanism of the antifertility action of minimal doses of megestrol acetate in the rabbit. 1. Experiments have been performed to investigate the mechanism by which low doses of megestrol acetate have an antifertility action.2. It was found that the dose of megestrol acetate required to prevent pregnancy in nine of ten New Zealand White rabbits when given on 3 consecutive days, the last of which was the day of insemination, was 50 mug/kg daily.3. This treatment reduced the numbers of sperm recoverable from the uterus at various times after insemination, but egg transfer experiments showed that neither egg viability nor fertilization was affected on day 1 of pregnancy.4. Treatment with megestrol acetate for 3 days caused the fertilized eggs to enter the uterus approximately 48 h prematurely. Treatment with megestrol acetate for longer periods caused a similar but more marked increase in the rate of transport of eggs in the oviduct.5. Megestrol acetate did not modify oviduct opening pressure, but the volume of oviduct fluid secretion was reduced earlier and transport of fluid through the oviduct was accelerated compared with controls.6. Transfer of eggs on various days from the uteri of untreated rabbits to the uteri of treated rabbits indicated that the development of the endometrium which occurs in pregnancy may be advanced by megestrol administration. Histological examination of the endometrial proliferation induced by megestrol acetate confirmed that this was so.7. It is concluded that the antifertility action of megestrol acetate in doses that do not necessarily inhibit ovulation is due to a combination of accelerated egg transport and advanced endometrial development.


Effects of sulphydryl inhibitors on frog sartorius muscle: N-ethylmaleimide. 1. The characteristic action of the -SH inhibitor, N-ethylmaleimide (NEM), is muscle rigour. The dose-response curve indicates a biphasic effect with maximum rigour tension produced by 1.0 mM NEM; beyond 1.0 mM there was an inverse relationship between dose and response.2. NEM produces a membrane depolarization unrelated to rigour development.3. NEM causes a sustained increase in (45)Ca efflux from whole muscle. Pretreatment of a muscle with ethylenediamine tetra-acetic acid (EDTA, 5 mM) to remove membrane calcium does not alter the NEM induced (45)Ca efflux.4. It is suggested that the primary site of NEM action is inhibition of calcium uptake by the sarcoplasmic reticulum thereby producing rigour. At concentrations above 1.0 mM, NEM may affect the myofilaments.


Studies on the antibacterial and antifungal properties of 1, 4-naphthoquinones. 1. New halogenated 1,4-naphthoquinones were synthesized and together with other known 1,4-naphthoquinones, were screened for antibacterial activity by a turbidimetric method, and for antifungal activity by the diffusion method on agar plates.2. The half-wave potentials and the influence on the oxidative phosphorylation of some of these compounds were determined.3. 2-chloro-3,2'-chloro-ethyl-1,4-naphthoquinone (half-wave potential=-187 mV) was the most active compound, completely inhibiting cell respiration.4. While the natural active naphthoquinones, vitamin K and ubiquinones, possess, as substituent, the electron repelling methyl group, the microbiologically active 1,4-naphthoquinones are substituted, in the quinone moiety, with electron attracting groups such as OH or Cl.5. The half-wave potentials can give only an initial indication of the activity of the compounds studied; a good correlation, on the contrary, can be found between the ultraviolet spectra of such compounds and their activity which seems to depend on the ability of active compounds to exist in an extensively conjugated structure and to form hydrogen bonds.


The influence of iron on the biosynthesis of porphyrins and the prosthetic group of cytochrome b in Achromobacter metalcaligenes.


Discontinuation of chemotherapy for chronic schizophrenics.


Radiotherapy of eye diseases.

Radiotherapy of eye diseases.


Sensitivity tests.

Sensitivity tests.


Rifampicin in the treatment of experimental tuberculosis in mice: sterilization of tubercle bacilli in the tissues.


Suitability of rifampicin for intermittent administration in the treatment of tuberculosis.


The structural basis of altered hepatic function in viral hepatitis.


Nitrogenase activity in extracts of heterocystous and non-heterocystous blue-green algae.


The disc and plate dilution methods in determination of the sensitivity of Neisseria gonorrhoeae to penicillin G, ampicillin and doxycycline.


Medical care in the extended care facility.


Family care as a useful alternative to the long-term hospital confinement of geropsychiatric patients.


Post-traumatic subarticular cyst of bone. A case report.


Formation of leukocyte inclusions in normal polymorphonuclear cells incubated with synovial fluid.


DNA antibodies in childhood scleroderma.


The occurrence of cryoproteins in synovial fluid; the association of a complement-fixing activity in rheumatoid synovial fluid with cold-precipitable protein. A significant portion of a complement-fixing activity found in the synovial fluid of patients with rheumatoid arthritis, and previously implicated as a possible cause of the low synovial fluid complement levels in these patients behaves as a high solubility cryoprotein. Analysis of rheumatoid synovial fluid cryoproteins has revealed mixed immunoglobulins, bound complement components, fibrinogen, DNA, and rheumatoid factor. Sorbitol density gradient studies on whole synovial fluid before and after removal of this activity has shown that the complement-fixing activity migrates in the 19S and heavier regions and that portion is removed with cryoprecipitation. Cryoproteins found in nonrheumatoid synovial fluid are generally devoid of complement-fixing activity and predominantly contain fibrinogen. DNA and IgG are also present, with IgG occurring significantly less frequently than in rheumatoid cryoproteins. These findings are discussed in relationship to recent studies demonstrating the presence of complement-fixing antibody to denatured DNA in rheumatoid cryoproteins.


Association of autoantibodies to different nuclear antigens with clinical patterns of rheumatic disease and responsiveness to therapy. Using a hemagglutination test which can detect antibodies to (a) native and denatured deoxyribonucleic acid (DNA) and (b) an extractable nuclear antigen (ENA), a comparative study of patterns of autoantibody formation has been done in systemic lupus erythematosus (SLE) and related rheumatic diseases. Antibody to native DNA was present in the serum in 96% of patients with active SLE and disappeared during remissions. Antibody to ENA was found in 86% of those patients with SLE nephritis who responded to treatment but in only 8% of those who did not. The highest titers of antibody to ENA were found in patients having a mixed connective tissue disease syndrome with features of SLE, scleroderma, and myositis. The latter syndrome was notable for the absence of renal disease and for a striking responsiveness to corticosteroid therapy. Hemagglutination testing of 277 sera from normal persons and patients with a wide variety of acute diseases other than SLE revealed the presence of antibody to native DNA in only 1.4% and antibody to ENA in only 0.4%. These results yield significant correlations among the pattern of autoimmune reactivity, the clinical form of the rheumatic disease, and responsiveness to treatment. They implicate the qualitative nature of the patient's immune response as a conditioning factor in the type of disease. Together with other correlations they may allow classification of rheumatic diseases into more biologically meaningful groups and lead to more selective methods of therapy.


The complement system in rheumatoid synovitis. I. An analysis of complement component activities in rheumatoid synovial fluids.


Folates in plasma and bile of man after feeding folic acid--3H and 5-formyltetrahydrofolate (folinic acid). During the 1st hr after feeding folic acid-(3)H ((3)H-PteGlu) to fasting human volunteers, plasma S. faecalis and (3)H activity were elevated to an equivalent degree, whereas after this, the (3)H activity exceeded S. faecalis activity, which suggests gradual conversion of folic acid-(3)H to methyltetrahydrofolate-(3)H (5-CH(3)H(4) PteGlu). The increase of L. casei activity exceeded the increase of S. faecalis and (3)H activity, which is consistent with flushing of endogenous methyltetrahydrofolate from the tissues by the administered folic acid-(3)H. Feeding of 5-formyltetrahydrofolate (+/-5CHOH(4)PteGlu) produced a large increase of plasma L. casei activity and only a slight increase of S. faecalis and P. cerevisiae activity, which is consistent with very rapid conversion of folinic acid to methyltetrahydrofolate. Bile folate concentration determined microbiologically was 2.3-9.8 times plasma folate. 40-80% of the bile folate was S. faecalis-active and 20-35% P. cerevisiae-active. Chromatography of bile folates on TEAE-cellulose showed several folates including four tentatively identified as 10-formyltetrahydrofolate (10-CHO-H(4)PteGlu), 10-formylfolate (10-CHO-PteGlu), and/or 10-formyldihydrofolate (10-CHOH(2)PteGlu), methyltetrahydrofolate, and possibly a triglutamate folate. After folate ingestion bile folate concentration increased rapidly. The distribution of bile folates measured by microbiological assay was similar after either folic or folinic acid feeding. Most of the (3)H label of folic acid-(3)H appeared in the biological folates of bile rather than in the folic acid fraction, which shows that the administered folic acid was rapidly transformed to other folates. Folate polyglutamate deconjugating enzyme activity was found to be much less than in serum. Polyglutamates of the type found in yeast were not found in bile. It is suggested that biliary folate may reflect the hepatic intracellular oligoglutamate folate pool rather than the folate as it appears in the hepatic portal blood.


Effect of Prymnesium parvum toxin, cetyltrimethylammonium bromide and sodium dodecyl sulphate on bacteria.


Syntheses and biological activity of 5-aminoimidazoles and 5-triazenoimidazoles.


Antimetabolite activity of some N,N-dialkylamino-1,2,3-triazoles and alpha-diazoamidines.


LPP-1 infection of the blue-green alga Plectonema boryanum. I. Electron microscopy. One-step growth and intracellular growth experiments were performed at high multiplicities of the virus LPP-1 during the infection of the blue-green alga Plectonema boryanum. The eclipse period lasts until 4 hr after infection, the latent period terminates at 6 hr, and the rise period continues until 14 to 16 hr after infection. The burst size was independent of multiplicity of infection over the ranges from 1 to 50. The burst size was 3,000 to 5,000 plaque-forming units (PFU) per infectious center or about 200 PFU per cell. Samples for electron microscopy were taken at characteristic times during the lytic cycle. The first sign of viral infection was the invagination of the photosynthetic lamellae at 3 hr after infection. Mature virions were visible at 4 hr. By 6 to 7 hr, many mature intracellular viral particles could be seen, with lysis beginning at 7 hr. By 10 hr after infection, all infected cells contained mature virions. No evidence for mass migration of performed viral precursors was obtained. The invagination of the lamellae could be prevented by the early addition of chloramphenicol, which implies that this process requires protein synthesis.


Immunological basis of the adenovirus 8-9 cross-reaction. The dedecon and hexon components of adenovirus types 8 and 9 have been extensively purified for use in establishing the basis of the cross-reaction between these types. Dodecons, the complete hemagglutinins, were purified 304- to 362-fold by fluorocarbon extraction, calcium phosphate batch chromatography, and ion-exchange column chromatography. Hexons, the group complement-fixation (CF) antigens, were purified 230- to 240-fold by erythrocyte adsorption, ion-exchange chromatography, and exclusion chromatography. Component antisera prepared in rabbits were tested in reciprocal fashion with crude virus and dodecon and hexon components. By hemagglutination-inhibition (HI), the dodecons of types 8 and 9 demonstrated the same predominantly one-sided relationship characteristic of the crude antigens. Some neutralizing activity was associated with both dodecons and hexons of each type. However, combining anti-dodecon and anti-hexon sera or producing antisera against the combined dodecon-hexon components resulted in neutralizing titers which were identical to titers obtained with antisera against the crude virus harvests. Dedecons of each type appear to share at least one antigenic determinant with hexons of the same type, and this determinant may reside on the vertex capsomere. Hexons possess group- and type-specific determinants, as shown by CF, neutralization, and immunodiffusion tests, and may exhibit some minor relationship between types 8 and 9. The results with the purified components are consistent with the predominantly one-sided antigenic relationship between types 8 and 9 in the conventional HI tests and the largely type-specific relationship by neutralization tests.


LPP-1 infection of the blue-green alga Plectonema boryanum. II. Viral deoxyribonucleic acid synthesis and host deoxyribonucleic acid breakdown. Host and viral deoxyribonucleic acid (DNA) metabolism in LPP-1-infected Plectonema boryanum was studied by equilibrium centrifugation in CsCl gradients. Approximately 50% of the host DNA is degraded to acid-soluble material between 3 and 7 hr after infection. Most of the acid-soluble product is reincorporated into viral DNA. Incorporation of exogenous (3)H-adenine into viral DNA can be detected very early after infection (within the first 2 hr), but the bulk of viral DNA synthesis occurs between 6 and 8 hr. Both the breakdown of host DNA and the synthesis of viral DNA require protein synthesis during the first few hours of infection.


LPP-1 infection of the blue-green alga Plectonema boryanum. 3. Protein synthesis. The structural proteins of mature LPP-1 particles and the patterns of protein synthesis after LPP-1 infection have been examined by electrophoresis on sodium dodecyl sulfate polyacrylamide gels. Structural proteins account for 35% of the LPP-1 genome, and proteins that would require about 65% of the total coding capacity have been detected after infection. The major head proteins have molecular weights of 39,000 and 13,000, whereas the major tail protein is an 80,000-molecular-weight species. Host protein synthesis is depressed soon after infection and appears to be entirely shut off by 5 hr. Three classes of viral proteins are distinguished in infected cells, based on their time course of synthesis and their presence in mature virions.


Equal incorporation of both parental bacteriophage T7 deoxyribonucleic acid strands into intracellular concatemeric deoxyribonucleic acid. After infection of Escherichia coli B with radiolabeled T7 bacteriophage, the parental deoxyribonucleic acid label was found in both polynucleotide chains of the intracellular T7 concatemer.


Developmental factor in the etiopathogenesis of glaucomatocyclitic crisis.


Biochemical and genetical studies on host parasite relationship: role of tyrosinase in pathogenicity and host resistance of two mutants of Fusarium vasinfectum Atk.


Parenteral feeding.

Parenteral feeding.


Spasmolytics.

Spasmolytics.


Antibiotics in the management of disease of the gut.


The rate of approach to equilibrium in uncatalyzed CO2 hydration reactions: the theoretical effect of buffering capacity.


Passive immunity in Plasmodium knowlesi malaria.


On the speed of the carotid chemoreceptor response in relation to the kinetics of CO2 hydration.


Examination of multiple sputum specimens in a tuberculosis survey.


ABO--Rh interaction. A study of five cases.


A lateral duodenal diverticulum.

A lateral duodenal diverticulum.


Report to the Minister of National Health and Welfare on the Recommendations of the Task Forces on the Cost of Health Services in Canada.


Behaviour of toxin released from the cells of Clostridium botulinum type E.


The role of the quinones prepared after oxidation of some amines by ceruloplasmin in the mitochondrial electron transport system.


Pressures for change. The financial pressures.


The effect of povidone-iodine in controlling skin flora beneath occlusive dressings.


In-vitro studies of rat viruses. I. Effects of long-term culture.


In-vitro studies of rat viruses. II. Effects of heat,x-irradiation and carcinogenic drugs.


Rescue plan for Indian monkeys.

Rescue plan for Indian monkeys.


Contours and contrast: responses of monkey lateral geniculate nucleus cells to luminance and color figures. The responses of single units in the monkey lateral geniculate nucleus to different portions of figures which differed from their backgrounds in color and brightness were examined. Border enhancement was found in the response to luminance figures but not in the response to color figures. In addition, cells showed border enhancement only in the case of a figure which produced an increment (as opposed to a decrement) in their firing rates. In situations in which very striking brightness contrast is seen perceptually, the cells do not show the corresponding changes in firing rate across the whole pattern. The lateral inhibitory mechanisms found in the retina and geniculate can thus account for luminance border enhancement, but not entirely for simultaneous brightness or color contrast, for which other cortical processes of some sort must be responsible.


Hemoglobin polymorphism.

Hemoglobin polymorphism.


The effect of bromotrifluoromethane on operant behavior in monkeys.


The absorption and clearance of disodium cromoglycate from the lung in rat, rabbit, and monkey.


Repeated and continuous exposure of laboratory animals to acrolein.


The stimulation and inhibition of hepatic microsomal drug-metabolizing enzymes with special reference to effects of environmental contaminants.


Poisoning due to heavy metals.

Poisoning due to heavy metals.


Human listeriosis in Hawaii. Report of a fatal case.


Ultrastructural study of characteristic organelles (paired organelles, micronemes, micropores) of sporozoa and related organisms.


Correlation of Rhesus (Rh) and personality factors.


Endocrinologic aspects of dysfunctional uterine bleeding.


The communication matrix of the Nilgiri langur (Presbytis johnii) of South India.


Thorough, objective perception is what keeps care humane.


The Task Force reports of the cost of health services in Canada...a response.


Suggestions for patient care.

Suggestions for patient care.


East Coast fever.

East Coast fever.


Transferrin saturation and erythropoietic activity in long-term hemodialyzed uremic patients.


Macroglobulinemia of Waldenström and the chromosomal morphology.


Electroanesthesia (EA), 1971.

Electroanesthesia (EA), 1971.


Life expectancy of head injured men with and without epilepsy.