The correlation between IgM anti-D and 'saline agglutination' of D-positive cells by anti-D sera.


Australia-SH antigen in hepatitis patients in London. Australia-SH antigen was found in 11 out of 27 sera (40%) obtained from patients with acute viral hepatitis soon after their admission to hospital. Six out of 11 positive sera were collected within the first 12 days of illness; die remaining five were collected between the 13th and 30th days. The antigen was detected by immunodiffusion in agarose gel, five different indicator sera containing Australia-SH antibodies being used. The specificity of these sera was found to be very similar.All of the patients' sera were examined independently by two separate laboratories. The results obtained by the laboratories were in close agreement, though the techniques varied in detail.


The serology of malaria: recent applications.


Selective depletion of caudate nucleus dopamine and serotonin during chronic manganese dioxide administration to squirrel monkeys.


Media-dependent antagonism of gentamicin sulfate by Liquoid (sodium polyanetholsulfonate).


The cellular sources of antibodies in diffusion chamber cultures of alveolar exudates.


A possible duplication in the X chromosome of man.


The distribution of tubercle bacilli in estrogen-treated mice.


Interaction of ethylenediaminetetraacetic acid with plasma proteins. II. Its effects on the binding of indium and chromium.


Carbonic anhydrase activity in ependymoglial cells of lower vertebrates.


Group practice prepayment plans. Universities give new impetus to old concept.


Reproductive behavior of socially deprived male rhesus monkeys (Macaca mulatta).


A possible storage form for tyrosinase substrates in Drosophila melanogaster.


Environmental deprivation: its effect on the growth of infant monkeys.


The hydrolysis of dipeptides and aminoacyl naphthylamides by the human seminal plasma.


Antigenic profile of rat vesiculo-prostate complex.


Comparison of vaginal tolerance tests of spermicidal preparations in rabbits and monkeys.


A case of cerebral cysticercosis.

A case of cerebral cysticercosis.


Some effects of material obtained from snake bite area.


Influence of bentonite, magnesium, and polyamines on degradation and aggregation of tobacco mosaic virus.


Removal of pentons from particles of adenovirus type 2.


Fine structure of the erythrocytic stages of Plasmodium knowlesi. A comparison between intracellular and free forms.


Growth repression of Mycobacterium tuberculosis in the presence of Krebs cycle intermediates and some other substrates.


Linkage of a temperature-sensitive locus to the streptomycin region and its use in recombination studies with streptomycin mutants of Bacillus subtilis.


Serological relationships in the Pycnoporus complex.


Lactate dehydrogenase isozymes: qualitative and quantitative changes during primate evolution.


Chemotherapy and comparative biochemistry: G. H. A. Clowes memorial lecture.


Isolation of a fetal antigen from human colonic tumors.


Inhibition of the growth of mouse melanoma by chlorpromazine.


Senile cataract: serum factors producing irreversible precipitation of a lens protein.


Thyroxine-binding proteins in the serum of the grey kangaroo.


The antigens of Mycoplasma hominis.

The antigens of Mycoplasma hominis.


Antibody response following smallpox vaccination and revaccination.


Antibody response in non-haemorrhagic smallpox patients.


The serological response and long-lasting resistance against infection with louping-ill virus in sheep immunized with a highly attenuated tick-borne encephalitis virus.


Differentiation of rabbit sperm antigens from those of seminal plasma.


Characterization and isolation of a sperm-coating antigen from rabbit seminal plasma with capacity to block fertilization.


A field trial of further attenuated live measles virus vaccines in Japan, 1968.


Serological identification of bovine epizootic fever virus as ephemeral fever virus.


Base compositions of DNA from some malarial parasites.


The effect of cytosine arabinoside on nucleic acid synthesis in normal and leukaemic human leucocytes in vitro.


Vitamin B12 absorption test.

Vitamin B12 absorption test.


Glucose-6-phosphate dehydrogenase deficiency in Italy. A study of the distribution and severity of the enzymatic defect.


Functional and cellular alterations produced by phytohemagglutinin. 3. Hematologic findings in mice receiving PHA.


Effect of lysolecithin on bovine erythrocyte osmotic fragility.


Hereditary deficiency of peroxidase and phospholipids in eosinophilic granulocytes.


Hereditary deficiency of adenylate kinase in red blood cell.


Suppression by RNA of human lymphocyte transformation.


Distribution of amino acids in urine, plasma, leucocytes and erythrocytes of leukaemic and normal subjects.


Immunochemical studies on O-antigens of Vibrio parahaemolyticus. 2. Effect of alkali and acid on specificity.


The starch gel quantitative electrophoresis of haemoglobin as screening in the thalassaemia syndromes.


Electron microscopy of rubella infected BHK21 and VERO cells.


Blood supply of the optic nerve head and its role in optic atrophy, glaucoma, and oedema of the optic disc.


Blood supply of the ciliary ganglion in the rhesus monkey.


A simple method for the determination of EDTA in serum and urine.


An improved small-scale procedure for CPK determination.


Serum luteinizing hormone and progesterone concentration during the menstrual cycle of the rhesus monkey.


Problems in the quantitative estimation of human serum haptoglobin by single radial immunodiffusion.


A TBG-deficient family with a male exhibiting decreased but not zero TBG levels.


Serum diamine oxidase in pregnancy and in trophoblastic diseases.


Increased serum thyroxine-binding globulin capacity: inheritance and linkage relationships.


Effects of localized cooling on spinal cord trauma.


The "sprained" ankle.

The "sprained" ankle.


Effect of alcohol and various diseases on leukocyte mobilization, phagocytosis and intracellular bacterial killing.


Serological and electron microscopic characterization of a new group of blue-green algal viruses (LPP-2).


Failure of a replicating adenovirus to enhance adeno-associated satellite virus replication.


The epidemiology of meningococcal meningitis in the Netherlands in recent years, with special reference to the epidemic of 1966.


Leukocyte alkaline phosphatase. The effect of age and sex.


Absorption and excretion of cyanocobalamine after oral administration of a large dose in various conditions.


Lymphopoietin.

Lymphopoietin.


Cell-degeneration pattern in bone marrow and blood of guinea pigs following administration of cyclophosphamide.


Histiocytic medullary reticulosis. Case report with electron microscopic study.


Effect of reserpine on accommodation and pupil diameter in monkeys (Macaca irus).


A case of acute leukemia with pseudo-Pelger cells containing Auer Bodies.


Pancreaticoduodenectomy for traumatic lesions.


Studies on the effect of permeant anions on the reversal of Ca++-induced mitochondrial alkalinity at different pH values.


The effect of electroconvulsive shock on a conditioned emotional response in the rhesus monkey.


The effect of delayed dehydration of carbonic acid on renal bicarbonate clearance, and its significance for acid-base balance.


The effect of lead and ferrous and ferric iron on delta-aminolaevulic acid synthetase.


Breath acetone and blood sugar measurements in diabetes.


Haemagglutination test to detect tobacco mosaic virus antigens.


Effects of bacterial contamination on haemagglutination-inhibition test with two arbovirus antigens.


Effect of exogenous gonadotropins on the ovary of juvenile langurs, Presbytis entellus entellus Dufresne.


Electron microscopic studies of novobiocin treated Bacillus subtilis.


Effect of actidione on the cell cycle in Stylonychia notophora (Stokes).


Uterine bleeding in amenorrhic rhesus monkeys after intrauterine contraceptive device insertion.


Variation in ascorbic acid content & ascorbate-oxidase activity during germination in dark of gamma-irradiated castor seeds.


Aids in diagnosis of swine fever.

Aids in diagnosis of swine fever.


The nature of nonlimbic learning.

The nature of nonlimbic learning.


Ligation of major thoraco-lumbar spinal cord arteries in monkeys.


Diverticuli of the nerve root sheaths. Case report.


Absorbed dose calculations for 113mIn placental scanning.


A new electrode holder for experimental electroanesthesia.


Self-administration of psychoactive substances by the monkey.


Intra-uterine transfusion.

Intra-uterine transfusion.


The red cell phenotype En(a-) and anti-Ena: serological and physicochemical aspects.


Rh antibodies in liquor amnii.

Rh antibodies in liquor amnii.


The properties of beams from the SL75 linear accelerator.


Properties of beams from the SL75 linear accelerator.


Problems of infection and antimicrobials relating to anesthesia and inhalation therapy.


Aspects on suppressability of aqueous humour formation.


Treatment of endotoxin shock.

Treatment of endotoxin shock.


The effect of trabeculotomy in normal eyes of rhesus and cynomolgus monkeys studied by anterior chamber perfusion.


Stress urinary incontinence in the human female. Gravity and ecomorphologic influences on bladder and urethral function of the human female.


On the mechanism of action of bleomycin: scission of DNA strands in vitro and in vivo.


Hypolipidemic agents.

Hypolipidemic agents. Despite the fact that the role of lipids and cholesterol in atherosclerosis has not been totally clarified to the clinician's satisfaction, medicinal chemistry has been very successful in this last decade in showing that a diverse group of compounds is capable of reducing blood lipid levels in many animal species and in humans.  The pharmacological basis for the evaluation of many hypolipidemic agents is summarized.  The prostaglandins are vasodepressor agents.  They inhibit uterine contraction, yet, on the other hand, they increase cardiac output and induce contraction of intestinal smooth muscle.  Prostaglandins exhibit marked antilipolytic activity.  It has been shown that 0.2 mug/kg/min, of intravenous infusion of prostaglandin E1 produced an increase in serum-free fatty acide levels in the dog.  At a 4 times higher rate of infusion, the plasma-free fatty acid concentration was lowered.  The pharmacological activities of individual prostaglandins embrace a great variety of biological phenomena.  In certain systems their effects are antagonistic.  The rationale for the use of estrogens as hypolipidemics is the observation of the low incidence of atherosclerotic heart disease in Caucasian women of childbearing age.


Cotransduction and cotransformation of genetic markers in Bacillus subtilis and Bacillus licheniformis. Bacteriophage SP-15, a large generalized transducing phage of Bacillus, was compared with phages PBS-1 and SP-10 for the ability to cotransduce pairs of genetic markers exhibiting different degrees of linkage. When auxotrophs of B. subtilis W-23 were used as recipients, SP-15 and PBS-1 effected a much higher frequency of cotransduction than did SP-10 with markers that were not closely linked. With more closely linked loci, the differences were not as great. SP-15 cotransduced linked markers at a higher mean frequency than PBS-1, suggesting that SP-15 is able to transfer a larger fragment of the Bacillus genome than any phage heretofore described. The frequency of the joint transfer of genetic markers in B. licheniformis was lower via transforming deoxyribonucleic acid than by transduction with phage SP-10. The availability of three procedures for genetic exchange-transduction by SP-15 and SP-10 as well as transformation-each of which reveals a different degree of linkage, makes B. licheniformis 9945A especially amenable to genetic analysis.


Comparative study of the structure of gas vacuoles. The fine structure of gas vacuoles was examined in two blue-green algae, two green bacteria, three purple sulfur bacteria, and two halobacteria. The gas vacuole is a compound organelle, composed of a variable number of gas vesicles. These are closed, cylindrical, gas-containing structures with conical ends, about 80 to 100 nm in width and of variable length, ranging from 0.2 to over 1.0 mum. The wall of the gas vesicle is a non-unit membrane 2 to 3 nm in thickness, bearing very regular striations with a periodicity of 4 nm, oriented more or less at right angles to the long axis of the cylinder. This fine structure could be clearly resolved in isolated gas vesicles prepared from a blue-green alga and from Halobacterium halobium, and its presence in the gas vesicles of the green bacterium Pelodictyon clathratiforme was inferred from thin sections. The gas vacuole thus appears to be a homologous organelle in all of these procaryotic groups. Minor differences with respect to the length and arrangement of the gas vesicles were observed. In blue-green algae and green bacteria, the vesicles are relatively long and tend to be arrayed in parallel bundles; in purple sulfur bacteria and Halobacterium, they are shorter and more irregularly distributed in the cell.


Effect of carbon dioxide on the aspartic acid requirement for proteinase biosynthesis by Streptococcus faecalis var. liquefaciens. Non-proliferating cells of Streptococcus faecalis var. liquefaciens required aspartic acid for proteinase biosynthesis in the absence of CO(2) but not in the presence of CO(2).


A closed system audio helmet for monkeys.


Punishment of shock-induced aggression.

Punishment of shock-induced aggression. Squirrel monkeys were restrained in a chair equipped with a tail-shock apparatus and a pneumatic bite hose located in front of the subject's face. An aggressive response was recorded when the monkey bit the hose. Initial sessions in which no shocks were delivered produced some biting. When biting during these sessions stabilized at a near-zero level, regularly scheduled shocks were delivered to the monkey's tail, causing a consistently higher rate of biting. After several sessions under these conditions, a punishment phase was introduced in which the previous shock conditions were maintained, and every bite was followed immediately by another, more intense shock. Biting under these conditions was suppressed to a near-zero level. When the punishment contingency was removed, biting increased. With one subject, two additional bite-contingent stimuli were examined: (1) a milder shock that, when made contingent upon hose biting, also suppressed that response, and (2) a contingent tone that had no obvious suppressing or facilitating effect. Individual differences among subjects were extreme, but the effect of bite-contingent shock was consistent. Observations of the subjects during the punishment sessions indicated the existence of certain side effects that resulted from the use of punishment to suppress shock-induced aggression.


Effects of d-amphetamine on observing behavior in the squirrel monkey. Four squirrel monkeys were trained to press a lever, which produced stimuli indicating availability or non-availability of reinforcement for pushing a key. Food reinforcements were available for the key response at random intervals with an average rate of 1 per min. When food was available, a single lever response produced a red light behind the key. Reinforcement availabilities and red keylights remained until terminated by a reinforced key response. When reinforcement was not available, each lever response produced a 0.5-sec green light on the key. Except after lever responses, the key remained dark. Under this procedure, lever responses functioned as observing behavior in that they produced discriminative stimuli correlated with the availability or non-availability of reinforcement for key responses. The procedure generated a high rate of responding on the lever, short latencies of the key response after onset of red lights and few responses to the key in the absence of red lights. Intra-muscular d-amphetamine, in doses from 0.125 to 1.0 mg/kg, abolished both observing behavior and key responding for periods that increased as a function of dose. However, both observing and key rates were increased at the smallest dose in two subjects whose performances included responding to the key in the absence of red lights. Results are discussed in relation to previous findings regarding effects of amphetamines on operant behavior and on observing and monitoring performance.


Transformation of phage-sensitivity in Bacillus subtilis.


The serological identity of Sabin's murine type C mycoplasma and Mycoplasma pulmonis.


Bacteriological and serological studies of fast growing mycobacteria identified as Mycobacterium friedmannii.


Activation of complement by a monoclonal cryoglobulin associated with cold urticaria.


Age dependent changes of certain peptide hydrolases and dehydrogenases in bovine dental pulp.


The hazardous effects of laser radiation on the eye.


Experimental ischemia in monkeys. II. Vascular response.


Mace, a dangerous police weapon.

Mace, a dangerous police weapon.


Ocular complications of chlorpromazine therapy.


Changes in human ERG induced by intravenous alcohol.


Visual receptive fields of neurons in inferotemporal cortex of the monkey. Neurons in inferotemporal cortex (area TE) of the monkey had visual receptive fields which were very large (greater than 10 by 10 degrees) and almost always included the fovea. Some extended well into both halves of the visual field, while others were confined to the ipsilateral or contralateral side. These neurons were differentially sensitive to several of the following dimensions of the stimulus: size and shape, color, orientation, and direction of movement.


Detergents: side effects of the washday miracles.


Intraperitoneal drainage.

Intraperitoneal drainage.


Jejunostomy for feeding or decompression.


Dental group practice, why, when and where?


Cervical esophagostomy.

Cervical esophagostomy.


Is compulsive continuing education the answer?


Caries and tribal food habits: applying the findings to Connecticut children.


U. Conn. School of Dental Medicine.

U. Conn. School of Dental Medicine.


Case report. Dental impression material as an aid for the excision of mucosal cysts.


Considerations on diverticulosis of the small intestine.


Oral medicine. 20. Some diseases of the oral mucosa.


A new fluid medium for cultivating gonococci.


Thinking translated into action.

Thinking translated into action.


Poisons at work.

Poisons at work.


Address to graduating nurses.

Address to graduating nurses.


Dynamics of ideas--is there a place for ideas in nursing?


Thought and action in the professional organization.


Noise--the invisible enemy.

Noise--the invisible enemy.


Cyanide poisoning; kelocyanor--a new treatment.


Editorial.

Editorial.


Professional observations in North America.


Blood proliferocytes in human and animal malaria.


Transketolase activity of red blood cells from infancy to old age.


Haemoglobin L Persian Gulf: alpha-57 (E6) glycine leads to arginine.


Haptoglobin in thalassemia.

Haptoglobin in thalassemia.


Evaluation of a dual radioisotope urinary excretion test in the diagnosis of pernicious anaemia.


Man, ecology, and health.

Man, ecology, and health.


Studies on bacillary dissemination in normal and immunized animals.


The baboon and experimental cholelithiasis.


The production of biliary cirrhosis in Macaca mulatta monkeys.


Effects of prenatal x-irradiation on the ontogenesis of acetylcholinesterase and carbonic anhydrase in rat central nervous system.


Regional differences in accumulation of tritium-labeled norepinephrine, 5-hydroxytryptamine and gamma-aminobutyric acid in brain slices of spider and rhesus monkey.


Interrelationships of blood sugar and ketones in insulin-treated diabetics. A survey of ketonuria in insulin-treated diabetics showed that its significance might vary according to the time of day at which the test was performed. Some of the patients had uncontrolled diabetes in the early morning, when severe hyperglycaemia and hyperketonaemia occurred together, while later during the same day or night an episode of hypoglycaemia caused hyperketonaemia, indicating that too much insulin had been given. Correct assessment of the significance of ketonuria is obviously important, because some patients would probably require a decrease rather than an increase of insulin dosage. Ketonuria does not necessarily indicate impending ketoacidosis.


Long-term care.

Long-term care.


Anti-adrenal cellular hypersensitivity in Addison's disease. 3. Species-specificity and subcellular localization of the antigen.


Post-mortem arteriography of cerebral arteriovenous malformations.


Rhodanine: a selective inhibitor of the multiplication of echovirus 12. A search for compounds which have previously unrecognized antiviral activity led to the discovery that rhodanine inhibits the multiplication of echovirus 12 and also the development of virus-induced morphologic changes. Eighteen derivatives and analogs of rhodanine were synthesized and tested against echovirus 12. These compounds were considerably less active than rhodanine or were inactive, and some of them were more toxic to the host cells than rhodanine.


Epidermal cell proliferation during the first 24 hr after injection of an aqueous skin extract (Chalone).


Lysosomal hydrolases in experimental amyloidosis. II. Effects of dimethylsulfoxide in vivo and in vitro.


The melanocyte: its cytochemical and immunological relationship to cells of the endocrine polypeptide (APUD) series.


Clearance of Rh-positive foetal erythrocytes from the blood of Rh-negative adults by intravenously applicable immunoglobulin G anti-D.


Rh-D antibody titer in amniotic fluids. Its use as an index of the severity of erythroblastosis fetalis. Report of a study of Rh antibody titers in the amniotic fluids from 42 Rh-negative sensitized pregnancies is presented.  Amniocentesis was performed in the pregnant Rh-negative women having serum Rh antibody titers (indirect Coombs) ranging from 1/64 to 1/4,096.  Spectrophotometric analysis was done on the antiRh-D titers.  In order to predict the outcome of pregnancy based on the amniotic fluid Rh antibody content, all fetuses were divided into 3 categories.  Those with titers of 1/4 or below should correspond to Category I; unaffected or mildly affected babies; those with titers of 1/8 to 1/16 should be in Category II or have moderate to severe disease; while fetuses with titers of 1/32 or above  would be in Category III indicating very severe disease.  Out of 18 cases with titers of 1/4 or below, 17 were unaffected or mildly affected.  In 10 patients titers of 1/8 or 1/16 were observed as the highest titer.  8 infants in this group were moderately to severely affected.  14 cases were observed with titers of 1/32 or above and in 11 of them, the pregnancy outcome was a severely ill or dead infant.  The other 3 cases in this group were babies with severe anemia who required 2 or 3 exchange transfusions.  It was learned that the antibody titer of amniotic fluid of Rh-sensitized pregnancies can be used as a reliable index of the severity of hemolytic disease in the fetus.


The complement-fixation test in extracutaneous sporotrichosis.


The serologic diagnosis of parasitic infections in medical practice.


Validity studies of the radioactive microsphere method for the study of the distribution of cardiac output, orn blood flow, and resistance in the conscious rhesus monkey.


Sensitization of guinea-pigs to measles vaccines.


The electroencephalogram as a diagnostic aid in neurosurgery: a review.


The antigenic independence of the three aspartokinases of Escherichia coli K 12.


Separation of two types of light polypeptide chains from rabbit gamma-globulins.


Kidney antigens active in isoimmunization. I. The antigenic diversity.


On the inhibition of aspartate aminotransferase by its antibody.


Interactions between some aural aspergillus species and bacteria.


Patterns and sequences of hitting behavior in mother and infant monkeys (Macaca nemestrina).


Distinct slime polysaccharide depolymerases of bacteriophage-infected Pseudomonas aeruginosa: evidence of close association with the structured bacteriophage particle. Five new polysaccharide depolymerases were isolated from cultures of Pseudomonas aeruginosa infected with phages 6, 7, 8, 9, and 10. The production of enzyme paralleled the release of phage. Depolymerase associated with phage 8 was active on slime polysaccharide A, whereas depolymerases associated with phages 6, 7, 9, and 10, like pseudomonas phage 2, hydrolyzed slime polysaccharide B. None of the depolymerases was active on slime polysaccharide C. Despite exhaustive purification, depolymerase activity was found to band with the phage particles at a density of 1.49 to 1.51 g/ml in a density gradient composed to cesium chloride. These results suggest that the depolymerases are firmly bound to the phage particles.


Host influence on the antigenic composition of the Kilham rat virus. The Kilham rat virus (RV) was found to vary in infectivity and antigenic characteristics when propagated in two different host systems. Both cross hemagglutination-inhibition (HI) and cross virus-neutralization tests revealed that a nonreciprocal or one way cross exists between RV propagated in rat embryo cell cultures (RE-RV) and RV propagated in suckling hamsters (H-RV). Specifically, immune serum to RE-RV inhibited hemagglutination and infectivity by both viruses to the same extent. Immune serum to H-RV, however, exhibited higher HI and neutralization titers to H-RV than to RE-RV. Infectivity studies demonstrated that, although both viruses were able to infect either host, the virus showed a higher infectivity titer for the last host in which it had been propagated. Serological studies indicated that H-RV possesses an antigen(s) not found on the RE-RV. This host-controlled variation did not result after a single passage in the new host, but rather required at least three passages for a complete conversion to occur, and did not appear to result from the incorporation of unaltered host antigens into the virus particle. Solubilization of purified RV propagated in each host with sodium dodecyl sulfate, 2-mercaptoethanol, and urea followed by electrophoresis in polyacrylamide gels indicated that the number of components in the protein of each virus was not the same.


Temperature-sensitive induction of bacteriophage in Bacillus subtilis 168. In a temperature-sensitive mutant of Bacillus subtilis 168, induction of the defective phage PBSX occurred at 48 C. Cell lysis began after 90 min of growth at 48 C, and cell viability began to decrease after 10 to 30 min. The loss in viability at the nonpermissive temperature was prevented by azide or cyanide. Deoxyribonucleic acid (DNA), ribonucleic acid, and protein synthesis were not inhibited at 48 C. Temperature induction of the temperate phage SPO2 also occurred in this mutant. The temperature-sensitive mutation, designated tsi-23, was linked by transduction to purB6 and pig, the order being purB6 pig tsi-23. Mutation tsi-23 was transformable to wild type by B. subtilis 168 DNA but not by DNA from the closely related strains W23 or S31. DNA from the latter two strains transformed auxotrophic markers of strain 168 at frequencies close to those found with 168 donor DNA. Upon temperature induction, cellular DNA was broken to a size of 22S, characteristic of DNA in PBSX particles. The DNA isolated from temperature-induced PBSX did not give an increased Ade(+)/Met(+) transformant ratio relative to cellular DNA nor contain preferential break points as determined by transformation of four closely linked markers.


Comparison of four horse herpesviruses.

Comparison of four horse herpesviruses. Four equine herpesviruses (equine abortion virus, equine herpesvirus types 2 and 3, and equine cytomegalovirus) were compared. The equine abortion virus did not cross-neutralize with any of the other viruses, but the other three did show varying degrees of cross-neutralization among themselves. Equine abortion virus grew more quickly in tissue cultures than did the others, and attained higher titers of infectivity in the culture fluid; it also formed plaques in a wider range of tissue culture species, although the other three were not specific for one tissue culture system only, in that they would multiply in rabbit and cat kidney cultures. The densities of the deoxyribonucleic acids of all four viruses were in the range 1.716 to 1.717 g/ml (a guanine plus cytosine content of 57 to 58%). Taxonomic separation, as a distinct serotype, of equine abortion virus from the other herpesviruses seems to be justified. The other three are closely related to one another. They should perhaps be regarded as separate viruses and termed horse herpesviruses types 2, 3, and 4, although an alternative view would be to regard them as variants of a single virus type. The question of whether types 2, 3, and 4, or any other herpesviruses, should be placed in a phylogenetically distinct subgroup, known as cytomegaloviruses, is a moot point.


Proteins of the group B arbovirus Kunjin. Purified Kunjin virus was disrupted with sodium dodecyl sulfate, urea, and mercaptoethanol or acetic acid. Electrophoresis on 7.5% polyacrylamide gel separated four proteins of high (120,000), intermediate (65,000) and low (18,000 and 13,000) molecular weights. A "core" particle was obtained by degradation of the virion with deoxycholate at 0 C; it contained the viral ribonucleic acid and the two small structural proteins. The "envelope" material released from around the core was identified with the most prominent (intermediate) protein seen in electropherograms of virion proteins. In addition to the structural proteins, at least three additional proteins (specified by virus infection) were found in the cytoplasm. The cytoplasmic proteins associated with Kunjin virus differed in their electrophoretic profile from those associated with infection by the related Murray Valley encephalitis virus.


Ketosis and metabolic alkalosis in a patient with diabetes.


Effect of thallium acetate on the growth of bacteria.


Ciliary orientation: controlled by cell membrane or by intracellular fibrils? The cirri of the ciliate Euplotes all asslumne the "reversed" orientation whenever the cell is depolarized and the "forward" orientation whenever the cell is hyperpolarized. Potenitial changes arise spontaneolusly or are induced by electrical or mechanical stimuli. The orientation responses of thte cirri are appatently independent of intracellular "neuromotor" fibrils previously assigned a coordinating function, as they persist after the fibrils are transected.


Drug effects on a discrete conditioned avoidance response in dogs, rhesus monkeys and rats.


Autonomic blockade in chronic Chagas' heart disease. Heart rate response at rest and during upright exercise. A preliminary report.


Cathepsins in pathology: an immunoenzymic study.


A new class of chromophoric organomercurials and their reactions with D-glyceraldehyde 3-phosphate dehydrogenase. THE SYNTHESES OF THE FOLLOWING ORGANOMERCURIALS ARE DESCRIBED: 2-chloromercuri-4-nitrophenol, 2-chloromercuri-4,6-dinitrophenol, 4-chloromercuri-2-nitrophenol and 2,6-dichloromercuri-4-nitrophenol. All four organomercurials show large spectral changes in the visible spectrum when thiols displace a more weakly bound ligand such as EDTA from the mercury atom. These spectral changes are primarily associated with pK perturbation of the nitrophenols. The mercurials are therefore chromophoric probes for thiol groups in proteins and other thiols of biological interest. The enzyme d-glyceraldehyde 3-phosphate dehydrogenase from lobster muscle is used as a model system in which the properties of the organomercurials may be illustrated. In particular it is shown how d-glyceraldehyde 3-phosphate dehydrogenase carboxymethylated at the active site may be mercurated at a specific site. This mercurial derivative may be crystallized and shown to be isomorphous with the parent enzyme. The mercurials also act as ;reporter groups' by monitoring phosphate or pyrophosphate binding to the enzyme. The mercurials may also be used to estimate cations by an EDTA displacement method.


Purification and properties of D-4-deoxy-5-oxoglucarate hydro-lyase (decarboxylating). 1. An enzyme extracted from Pseudomonas acidovorans was purified and shown to catalyse the simultaneous dehydration and decarboxylation of d-4-deoxy-5-oxoglucarate. It is proposed to name the enzyme d-4-deoxy-5-oxoglucarate hydro-lyase (decarboxylating), trivial name ;deoxyoxoglucarate dehydratase'. 2. No added cofactors were required, and the enzyme was inactivated when incubated with its substrate in the presence of sodium borohydride. Under these conditions the substrate and enzyme appeared to be bound covalently. 3. The action of the enzyme is readily explained if it is assumed that d-4-deoxy-5-oxoglucarate forms a Schiff base with a lysine residue in the enzyme.


Blood groups and genetic serum types in diabetes mellitus.


EDTA and insulin. A study of the effect of salts of EDTA upon insulin action in vivo and in vitro.


The role of the liver in the disposal of orally administered 14C-glucose in the normal rat.


Heterotopic ossification. A clinical study of its possible relationship to trauma.


Oral vaccination against tularemia in the monkeys.


A technique for estimation of platelet-collagen adhesion.


Characterization of the properties of mouse-adapted human measles virus.


Cyclamate and cyclohexylamine: transfer across the hemochorial placenta.


Epileptogenic action of penicillin.

Epileptogenic action of penicillin.


Jacksonian epilepsy.

Jacksonian epilepsy.


Heat activation kinetics of Bacillus megaterium spores.


Growth of Streptococcus faecalus in dense culture.


An active fragment of DNA polymerase produced by proteolytic cleavage.


Dosage regimen calculation of chemotherapeutic agents. V. 2-Sulfanilamido-3-methoxy-pyrazine (Sulfalene).


Long-term changes in a population of Ugandan baboons.


Current status of the polymer tooth implant concept.


Studies on menstrual cycles and other related phenomena in the langur (Presbytis entellus entellus).


Absorption of water from isolated ventral sac of rumen of the cow.


Respiratory blood gases and pH in two species of unanesthetized monkeys.


Biochemical studies of bacterial sporulation and germination. XVII. Sulfhydryl and disulfide levels in dormancy and germination. A fourfold increase in sulfhydryl content upon germination of Bacillus megaterium spores was observed by the standard fluorescein mercuric acetate assay as reported by others. However, assay of ruptured dormant spores or the use of N-ethylmaleimide and a denaturing agent on intact spores showed a constant sulfhydryl level in dormancy and in germination. The apparent increase in sulfhydryl groups observed on germination was shown to be due to inaccessibility of most sulfhydryl groups in the dormant spore to sulfhydryl reagents. The disulfide content of dormant spores showed no change on germination, nor was any evidence found for production of low-molecular-weight sulfhydryl or disulfide compounds during germination.


Results of nonoperative and operative treatment of fractures of the ankle.


The biochemistry and physiology of the intervertebral disk.


Pharmacologic properties of chymopapain.


Chemistry and end-group analysis on purified M protein of type 12 group A streptococcal cell walls. M protein was extracted from the cell walls of streptococci by use of both acidic and alkaline buffers. These extracts were further purified by ammonium sulfate fractionation and column chromatography. Both diethylaminoethyl and carboxymethyl celluloses were employed to cover the pH range of 3.0 to 9.0. All of the M proteins isolated were immunologically related, but their physical and chemical properties varied dependent upon the pH range of isolation. Each isolate appeared to be homogeneous on the basis of immunodiffusion analysis, electrophoretic mobility, and ultracentrifugal analysis, but their amino acid analyses differed slightly. Two factors were shared by all isolates: (i) they all reacted with type-specific antisera and (ii) each seemed to have l-lysine as a single N-terminal amino acid.


Isozymes of beta-glucosidase in Dictyostelium discoideum. The activity of beta-glucosidase (EC 3.2.1.21) in extracts of Dictyostelium discoideum was investigated. The specific activity increased early in development, declined during pseudoplasmodium formation, and increased again during sorocarp formation. The beta-glucosidase which was present in growing amoebae and during the first stages of multicellular development was electrophoretically distinct from the enzyme which accumulated during the final stages of morphogenesis. Ribonucleic acid synthesis and protein synthesis during development were required for the accumulation of the later isozyme. Analysis of beta-glucosidase activity in a number of morphological mutants suggests that the enzyme which accumulates late in morphogenesis is developmentally controlled.


Initial characterization of a temperature-sensitive rod--mutant of Bacillus subtilis. The general biological properties of a temperature-sensitive morphological mutant of Bacillus subtilis (168ts-200B) are described. At the restrictive temperature (45 C), cells grow as spheres which divide irregularly to form grapelike clusters. At the permissive temperature (30 C), the mutant grows as typical B. subtilis rods in short chains. A log-phase culture of rods (30 C) may be converted to spheres by transfer to 45 C. Reversion of spheres to rods occurs when the alternate temperature shift is made. Growth curves, deoxyribonucleic acid replication kinetics, and the morphology of mutant 168ts-200B are described.


Requirement for coenzyme A in the phosphoroclastic reaction of anaerobic bacteria. Various bacteria which degrade pyruvate by the phosphoroclastic reaction were examined with respect to the role of coenzyme A (CoA) in this reaction. The strictly anaerobic bacteria, which cleave pyruvate by the phosphoroclastic reaction characteristic of Clostridia, required catalytic levels of CoA for the CO(2)-pyruvate exchange and acetoin-forming portions of the phosphoroclastic reaction. These reactions were reversibly inhibited by the CoA analogue, desulfo-CoA. In contrast, using cell-free extracts of bacteria which degrade pyruvate by the coliform phosphoroclastic reaction (pyruvate formate-lyase), no requirement for CoA could be observed for the formate-pyruvate exchange reaction. It is suggested that CoA serves a regulatory function in the early portion of the clostridal type of phosphoroclastic reaction.


Protein synthesis in cell-free extracts of Streptococcus faecalis. If a cell-free extract of Streptococcus faecalis is fractionated by way of a 10 to 70% sucrose gradient, at least three areas are found capable of protein synthesis having a density greater than can be accounted for by association of individual ribosomes. These areas represent distinct "polysome" peaks rather than random distribution of polymers of varied length. They appear to be membrane subunits. In addition there is a further particle with a density of about 150S, incapable of protein synthesis but which is capable of stimulating protein synthesis in some of the larger fractions found by gradient centrifugation.


Decay of normal and 5-fluorouracil-substituted messenger ribonucleic acid of alkaline phosphatase in Escherichia coli. The decay of alkaline phosphatase messenger ribonucleic acid (mRNA) in Escherichia coli was studied in cells sensitized to actinomycin D by ethylenediaminetetra-acetate treatment. It was found that in the wild-type strain (K-10) as well as in a nonsense mutant (S26C200), which is phenotypically reversible by treatment with 5-fluorouracil, mRNA decays with a half-life of 2.0 to 2.5 min. Similarly, in a 3-min pulse of labeled 5-fluorouracil, 70% of the incorporated analogue is contained in the labile RNA fraction decomposing with a half-life of 1.8 min.


Microgermination of Bacillus cereus spores. The biphasic nature of germination curves of individual Bacillus cereus T spores was further characterized by assessing the effects of temperature, concentration of germinants, and some inorganic cations on microgermination. Temperature was shown to affect both phases of microgermination as well as the microlag period, whereas the concentration of l-alanine and supplementation with adenosine exerted a significant effect only on the microlag period. The germination curves of individual spores induced by inosine were also biphasic and resembled those of spores induced by l-alanine. High concentrations (0.1 m or higher) of calcium and other inorganic cations prolonged both phases of microgermination, particularly the second phase, and had a less pronounced effect on the microlag period. The second phase of microgermination was completely inhibited when spores were germinated either in the presence of 0.3 m CaCl(2) or at a temperature of 43 C; this inhibition was reversible. Observations on the germination of spore suspensions (kinetics of the release of dipicolinic acid and mucopeptides, loss of heat resistance, increase in stainability, decrease in turbidity and refractility) were interpreted on the basis of the biphasic nature of microgermination. Dye uptake by individual spores during germination appeared also to be a biphasic process.


Osmotic fragility of lysozyme- and thiol-treated Lactobacillus plantarum. Reduced but not oxidized thiols increased the sensitivity of lysozyme-treated cells of Lactobacillus plantarum to lysis by osmotic shock.


Determinants of gene expression during viral development.


Biosynthesis of low molecular weight (7S) and high molecular weight (19S) immunoglobulin M. The class of immunoglobulin M (IgM) characterized by high molecular weight proteins with a sedimentation coefficient of 19S, includes a smaller molecular form with an S(20,[unk]) of approximately 7. The synthetic origin of the 7S IgM was investigated by biosynthetic studies on bone marrow cells from three patients with macroglobulinemia whose sera contained 7S IgM and 19S IgM. Labeled 7S IgM and 19S IgM were identified in extracellular culture fluids by radioimmunochemical techniques. The separation of the two molecular forms of IgM by density-gradient ultracentrifugation of the culture fluids before radioimmunochemical analyses permitted the identification of both the labeled 7S IgM and 19S IgM. One patient's serum contained two separate and distinct 19S IgM proteins as well as 7S IgM. The use of specific isolated carrier IgM proteins permitted the radioimmunochemical detection of labeled 7S IgM and both 19S IgM proteins. The introduction of cycloheximide into a culture system effects the cessation of protein synthesis. The analyses of culture fluids harvested at timed intervals after the addition of cycloheximide revealed not only the stability of 19S IgM to intracellular proteolysis, but also provided evidence for a possible precursor-product relationship between the 7S IgM and the 19S IgM. The demonstration that the labeled 7S IgM is neither an in vitro breakdown product of 19S IgM nor a resultant of 19S IgM intracellular catabolism substantiated the synthetic origin of 7S IgM in human sera.


Freezing excysted coccidial sporozoites.


Infectivity of Plasmodium malariae in the Aotus trivirgatus monkey to Anopheles freeborni mosquitoes.


Research with the primate equilibrium platform in a radiation environment. SAM-TR-68-81.


Radioprotection with 2-(1 decylamino) ethanethiosulfuric acid in the rhesus monkey. SAM-TR-68-110.


Condylar growth in rhesus monkeys.

Condylar growth in rhesus monkeys.


Effects of endotoxin on cerebral blood flow in the monkey.


Redistribution of cardiac output after sodium pentobarbital anesthesia in the monkey.


Brain potential shift with respiratory acidosis in the cat and monkey.


Mechanisms of baroreceptor-induced changes in heart rate.


A study of adjuvant-induced polyarthritis in the rat with special reference to associated immunological phenomena.


Correlations of brain d-c shifts with changes in cerebral blood flow.


Scanning electron microscope studies of EDTA-treated enamel.


Polymorphism of monkey thyroxin-binding prealbumin (TBPA): mode of inheritance and hybridization.


Stimulation by prostaglandin E-1 of endocytosis and glucose oxidation in canine thyroid slices.


Plasma insulin response and glucose tolerance in pregnant rhesus monkeys.


Nutritional circulation in the heart. II. A reappraisal of the effect of nitroglycerin on myocardial hemodynamics, oxygen consumption and nutritional blood flow in the isolated supported heart preparation.


Multiple lytic bone lesions.

Multiple lytic bone lesions.


Pigments of pseudomonas species. I. Structure and synthesis of aeruginosin A.


Autoxidation of oxymyoglobins.

Autoxidation of oxymyoglobins.


Pigments of pseudomonas species. II. Structure of aeruginosin B.


Differences in antigenicity of human IgG produced by three established lymphocytoid cell lines from a single individual.


Human lymphocyte response to phytomitogens in vitro: normal, agammaglobulinemic and paraproteinemic individuals.


Reactivity of polymer (19S) and monomer (7S) forms of gamma-M with anti-gamma M serum.


Properties of purified mouse homocytotropic antibody.


Locally produced antibody in human gastrointestinal secretions.


Spontaneous thyroiditis in an obese strain of chickens. II. Investigations on the development of the disease.


Structural and antigenic relationships between avian immunoglobulins. I. The immune responses of pheasants and quail and reductive dissociation of their immunoglobulins.


Macroglobulin antibody response to Ascaris suum infection. Detection of a second macroglobulin component in the serum of infected mice.


Myxosporidea of some Brazilian freshwater fishes.


Abdominal exploration in the evaluation of patients with carcinoma of the thoracic esophagus.


Studies of the sudden infant death syndrome in King County, Washington. I. The role of viruses.


Comparison of the menstrual cycle of cynomolgus and Rhesus monkeys.


Metabolic considerations in the treatment of juvenile diabetes mellitus.


Fatal motorcycle accidents of military personnel: a study of 223 cases.


Intensive cardiac care: a study of professional nursing practice.


Biomedical study of military performance at high terrestrial elevation.


Hemostasis with cyanoacrylate: a new method.


General anesthesia for bronchoscopy.

General anesthesia for bronchoscopy.


Cardiopulmonary resuscitation in the pediatric patient.


Technique of methylmethacrylate cranioplasty utilizing the pneumatic craniotome.


Prevention of childhood aspirin poisoning.


Report of the 1968 seminar on Prophylaxis of Streptococcal Infection in the Armed Forces.


Composition C-4 induced seizures: a report of five cases.


Military psychiatry today.

Military psychiatry today.


Alpha--antitrypsin deficiency: a case report.


Glycogen synthetase, phosphorylase, and glycogen content of developing rhesus muscle.


Synthesis and secretion of placental lactogen and other proteins by the placenta.


Protein binding and the assay of nonantigenic hormones.


Phytochrome-mediated induction of ascorbic acid oxidase in mustard seedlings.


A comparison of methods for the quantitation of bacteria in burn wounds. I. Experimental evaluation.


Catecholamine stores under vagal control.


Definitions and classification of infectious reactions of the lung. A statement of the Committee on Diagnostic Standards in Respiratory Disease.


The pulmonary tuberculous lesion in North India: a study in medico-legal autopsies. II. Bacteriologic aspects.


The pulmonary tuberculous lesion in North India: a study in medico-legal autopsies. I. Incidence, nature, and evolution.


Correlation of human skin reactivity with lymphocyte transformation induced by mycobacterial antigens and histoplasmin.


Fundamental aspects of morphological melanin color changes in vertebrate skin.


Genetic disposition--a prerequisite for maternal rubella deafness.


A new light on the pathogenesis of the styloid syndrome.


Esophageal replacement by transposed stomach. Following pharyngolaryngo-esophagectomy for carcinoma of the cervical esophagus.


Coronary vascular effects of glucagon in the isolated dog heart.


Plasma insulin concentrations in dogs and monkeys after xylitol, glucose or tolbutamide infusion.


Benign and malignant sacrococcygeal teratomas.


Detection of silent colon cancer in routine examination.


An analysis of the formation of ciliary primordia in the hypotrich ciliate Urostyla weissei.


Cilia regeneration in Stentor: inhibition, delay and abnormalities induced by griseofulvin.


Mating types in Glaucoma scintillans.

Mating types in Glaucoma scintillans.


Carcinoma of the extrahepatic bile ducts.


Interdisciplinary approach to the management of head and neck cancer.


Scanning electron microscopy of gregarines (Protozoa, Sporozoa) and its contribution to the theory of gregarine movement.


The importance of routine sigmoidoscopic examination in asymptomatic patients.


The effects of temperature on Blepharisma intermedium.


Mating types in Stentor coeruleus.

Mating types in Stentor coeruleus.


Encomium.

Encomium.


Malignant tumors of the eye.

Malignant tumors of the eye.


Evidence for the multiplication of scrapie agent in cell culture.


Stereoscopic vision in macaque monkey. Cells sensitive to binocular depth in area 18 of the macaque monkey cortex.


Stereoscopic vision in the macaque monkey: a behavioural demonstration.


Mutagenic action of aflatoxin B1 on transforming DNA and inhibition of DNA template activity in vitro.


Primate sex pheromones of vaginal origin.


Congenital defects in guinea pigs: fetal resorptions, abortions, and malformations following induced hyperthermia during early gestation.


Congenital eye defects in the mouse. II. The influence of litter size, litter spacing, and suckling of offspring on risk of eye defects in C57BL mice.


Myeloperoxidase deficiency. Immunologic study of a genetic leukocyte defect.


Letters to the editor: effect of sodium cyclamate on protein-bound iodine.


Purification of a phospholipase C from Bacillus cereus.


Chronic obstructive pulmonary disease: considerations in treatment, from acute respiratory failure to long-term outpatient care.


The thalamic projection of hypoglossal afferents in the Rhesus monkey.


Further evidence on the locus of the visual area in the temporal lobe of the monkey.


Some subcortical connections of the inferotemporal gyrus of monkey.


Sex of schistosome cercariae as a factor in the immunization of rhesus monkeys.


Carbohydrate metabolism in Babesia rodhaini: differences in the metabolism of normal and infected rat erythrocytes.


The lability of a bovine Theileria species.


PAM 1392 (2,4-diamino-6-(3,4-dichlorobenzylamino) quinazoline) as a chemotherapeutic agent: Plasmodium berghei, P. cynomolgi, P.


Immunoprecipitins in Schistosoma mansoni infections. IV. Human infections.


Agar gel and immunoelectrophoretic analysis of several strains of Trypanosoma cruzi.


Chemotherapy of Plasmodium berghei.

Chemotherapy of Plasmodium berghei.


Trichinella spiralis: establishment in gnotobiotic mice affected by Bacillus mesentericus, B. subtilis, and Pseudomonas aeruginosa.


The location of chemical components on ultra-thin sections of Bacillus cereus embedded in glycol methacrylate.


The metabolism of starch, maltose, glucose and some other sugars by the rumen ciliate Entodinium caudatum.


N-acetyl-transferase and serotonin metabolism in man and other species. I.


Structure of the cytoplasmic membrane and mesosome.


Numerical taxonomy of Listeria, streptococci and possibly related bacteria.


The organisation of the polymers in Gram-positive and Gram-negative bacteria.


Adsorption and fate of adenovirus type 5 hexon antigen in HeLa cells.


Some properties of DNA in competent Bacillus subtilis.


Lipemia retinalis: microcirculatory changes and lipid studies in a family.


Plasmocytoma proteins.

Plasmocytoma proteins.


Observations on the generation time of Paneth cells in mice.


Graft rejection after lung transplantation. A radiologic-pathologic study in the baboon.


A biochemical and ultrastructural study of liver, muscle, heart and kidney in type II glycogenosis.


Histochemistry of acid phosphatase in murine hepatic bilharziasis.


Histochemistry of succinic dehydrogenase in murine hepatic bilharziasis.


Studies on crocker sarcoma under the effect of alkylating agents. I. Morpho-topochemical studies of Golgi apparatus and glycogen in malignant cells of sarcoma 180 after treatment with the antitumour drug DL-sarcolysine.


Structure of the rat aorta wall after the administration of 2-thio-6-azathymine.


On the distribution of acid phosphatase in chromosomal loci of drosophila annanase.


Studies on the effect of fixation on nervous tissue. IV. Volumetric changes of cerebellar structures due to preparation of the tissue for electron microscopy.


Myeloma proteins and antibodies.

Myeloma proteins and antibodies.


Giant retinal tears. II. Experimental production and management with intravitreal air.


Angioarchitecture of the optic nerve. I. The papilla.


Angioarchitecture of the optic nerve. II. Lamina cribrosa.


Double contrast gas barium cystography in the assessment of diverticula of the bladder.


The interaction of Escherichia coli endotoxin with leukocytes.


Studies on thyroxine-binding globulin (TBG). II. Separation from human serum by affinity chromatography.


Characterization of two hetero-beta-galactosidases from monkey small intestine.


On the specificity of Bacillus subtilis neutral protease in relation to the compound active site.


The structure of the terminal regions of the encephalitogenic basic protein from bovine myelin.


Amino acid composition and specificity of a keratinase of Trichophyton mentagrophytes.


The preparation and characterization of chicken erythrocyte nucleoproteins.


Influence of calcium removal on urecholine-induced changes in acid-soluble metabolites.


A comparison of the crosslinking abilities of glutaraldehyde, formaldehyde and alpha-hydroxyadipaldehyde with bovine serum albumin and casein.


The incidence and bacteriology of bacteriaemia--a study of two hospitals.


Microcirculatory changes and the cotton-wool spot.


Pathological and ultrastructural aspects of the cotton-wool spot.


Comparative aspects of amelogenesis imperfecta.


Tentorium cerebelli.

Tentorium cerebelli.


The effects of chlorpromazine and secobarbital on matching from sample and discrimination tasks in monkeys.


Amphetamine, scopolamine and chlorpromazine interactions on delayed matching performance in monkeys.


Fixed-ratio and fixed-interval control of responding, using noxious stimuli: effects of methylphenidate and reserpine.


Long term complete parenteral nutrition.


Immunofluorescent localization of lysozyme in human leukocytes.


Antimicrobial factors in amniotic fluid.


Monoclonal macroglobulinemia in malignant lymphoma.


The relation of human salivary gamma A globulin and albumin to flow rate.


Juvenile xanthogranuloma: ultrastructural study.


The association of rheumatoid arthritis with plasma cell and lymphocytic neoplasms.


Immunological reactions of the organic matrix of developing bovine enamel.


Epiphrenic esophageal diverticulum.

Epiphrenic esophageal diverticulum.


Biological activity of synthetic polypeptide derivatives related to the structure of hypothalamic TRF.


Effects of vagotomy or splanchnicotomy on blood insulin and sugar concentrations in the conscious monkey.


Thyroidal glycerokinase.

Thyroidal glycerokinase.


Compartmental analysis of xylose intestinal absorption in rabbits and of the effect of various drugs.


Diphenylhydantoin as an anticonvulsant: protein binding and fluctuation of the serum and cerebrospinal fluid levels in forty mentally subnormal epileptics.


Isolation of a cell membrane-DNA-nascent RNA complex from bacteria.


Feline leukaemia (lymphosarcoma) symposium. 3. Some comparative aspects of leukaemia in man and animals with particular reference to lymphosarcoma.


Partial nerve laceration evaluated by evoked potentials.


Immunologic studies of untreated and chemically modified bovine carotid arteries.


Immunoserological studies on myocardial infarction and postmyocardial infarction syndrome.


Calcium and permeability of ascites tumour cells.


An operative staging system and a megavoltage radiotherapeutic technic for cerebellar medulloblastomas.


Intravenous infusion of nitroglycerin in normal subjects.


Clinical and radiotherapeutic aspects of reticulum cell sarcoma.


Chromium -51- EDTA in the determination of glomerular filtration rate.


Selective uptake of a radioactive drug into human tumour cells growing in tissue culture.


Heterogeneity of maleylated and partially delipidated low density lipoproteins from human plasma.


Haptoglobin subtypes among Finnish Skolt Lapps.


Cross-reacting antigens in kidney and other organs.


Nonliving immunogenic substances of Mycobacteria.


Fracture dislocation of the ankle with fixed displacement of the fibula behind the tibia.


Present aspects of genitourinary tuberculosis.


The effects of inhibition of carbonic anhydrase with dichlorphenamide on ventilatory control at rest and on exercise in normal subjects.


Measurement of parathyroid hormone in man by radioimmunoassay.


The pyramidal tract: recent investigations on its morphology and function.


Species differences in acute retrograde neuronal reaction of the facial and hypoglossal nuclei.


Central distribution of the dorsal root of C-1 and accessory nerve of the marmoset monkey.


Histochemical method for the demonstration of the activity of glucosephosphate isomerase.


Influence of fixation procedures on the cytochemistry of neurons.


Histochemical demonstration of alkaline inorganic pyrophosphatase in male accessory sexual glands of the mouse, rat and guinea-pig.


Inframicroscopical localization of lipoic dehydrogenase.


Comparative investigations on cholinesterase activity in cranial and spinal motoneurons.


Mitochondria and the peculiar cytosomic ensemble of polyanionites in the medullated nerve fiber.


Experimental chemotherapy of mycobacterioses provoked by atypical mycobacteria.


On the effects of cyanide on phenoxazinone synthetase.


Common and species-specific serum esterases of Equidae. I. Horse and donkey.


Pyrrolidonecarboxylyl peptidase from rat liver.


Connexions of the somatic sensory cortex of the rhesus monkey. II. Contralateral cortical connexions.


An assessment of the value and limitations of air encephalography in children with mental retardation and with epilepsy.


The pretectal syndrome in monkeys. II. Spontaneous and induced nystagmus, and "lightning" eye movements.


Folate derivatives in healthy and rust-infected primary leaves of wheat.


The isolation and properties of the skin-reactive substance in Aedes aegypti oral secretion.


Multiple molecular forms of avian aldolases. 3. Tissue distribution and properties of the predominant isozymes from chicken (Gallus domesticus).


"Anion-gap acidosis" complicating diabetic ketoacidosis. A commentary on diagnosis and management.


Urethral diverticulum: a recondite disease.


Distribution of a dipeptide naphthylamidase in rat tissues and its localisation by using diazo coupling and labeled antibody techniques.


Licensing and disciplining boards exempt from Convention's reorganization proposal.


Comparative drug metabolism.

Comparative drug metabolism.


Recent advances in molecular pathology: a review. Some aspects of chromosome changes in cancer.


Cell transformation by the small DNA-containing viruses.


Characterization of a major allergen (cod). Effect of enzymic hydrolysis on the allergenic activity.


Effects of surgically reduced fracture dislocations of mandibular condyles on facial growth in Macaca rhesus monkeys.


Karl Landsteiner, M.D. History of Rh-Hr blood group system.


The effect of anti-D immunoglobulin in Rh-positive persons. A review of the effects of accidental administration of anti-D immunoglobulin.


Studies on human immunoglobulin G Fc sub-fragments. Structural requirements for biological expression.


A comparative study of spectrin: a protein isolated from red blood cell membranes.


Preparation of polymeric protein markers and an investigation of their behavior in sodium dodecyl sulfate-polyacrylamide gel electrophoresis.


The solubilization of mature, polymeric collagen fibrils by lyotropic relaxation.


Dermonecrotic factor. I. Nature and properties of a dermonecrotic factor to guinea-pig skin found in human serum.


Impaired discrimination following polarisation of the striate cortex.


The occurrence of antigenic determinants of human blood proteins in mammalian plasmas.


Low molecular weight antibodies in human sera to stratum corneum.


Tumor antigens. I. Immunological studies of tissue and tumor antigens and serum protein production by a mouse rhabdomyosarcoma and mammary adenocarcinoma.


Mycobacterial polysaccharides. I. Serological and skin testing studies on mycobacterial polysaccharides.


Studies of methods for quantitation of agglutinins and precipitins to Escherichia coli O and K antigens.


The antibody response in rabbits to E. coli O antigen.


Production of reaginic antibody, in monkeys, following immunization with dialyzable constituants of timothy pollen extracts.


The first example of auto-anti-M and its consequences in pregnancy.


The antibody response in rabbits to E. coli K antigen.


Relationships between antibodies and experimental allergic encephalomyelitis. 3. Coprecipitation and radioautography of 125I-labeled antigen-antibody complexes for detection of antibodies to myelin basic protein.


The occurrence of antibodies to stratum corneum in man.


A study of the behavior of propionyl erythromycin in blood by a new chromatographic method.


Influence of various parameters on the sensitivity of precipitin tests in farmer's lung by immunodiffusion and immunoosmophoresis. Dilutions of a standard serum were made in saline and in negative sera, and comparative tests by immunodiffusion and by immunoosmophoresis were made against one antigenic component of Micropolyspora faeni (Thermopolyspora polyspora). The endpoint dilution of the standard serum in saline and serum in wells of different sizes was ascertained in each test. The endpoint dilution was unaffected by the distance of separation (from 1.5 mm to 6 mm) between the origins of serum and antigen, provided that the tests were read at times appropriate to each separation, the greater separations requiring longer periods of development. Higher endpoint dilutions were obtained by the use of 1% Noble agar in immunoosmophoresis than with 1% Ionagar no. 2 (Oxoid). This difference was more noticeable when low osmophoresis potentials were used.


Precipitins with relevance to farmer's lung and aspergillosis in normal and other sera. Immunodiffusion, immunoosmophoresis, and precipitin inhibition tests were carried out on various categories of sera with extracts of Micropolyspora faeni (Thermopolyspora polyspora), Aspergillus fumigatus, and Thermoactinomyces vulgaris. The sera were from clinical cases of farmer's lung, from symptomless farm workers, from expectant mothers, from tuberculous and from asthmatic patients, and from patients with miscellaneous lung disorders. The clinical cases of farmer's lung became divisible into two groups by serological tests for antibody to a particular soluble antigenic component of M. faeni. The suggestion is made that one of these two groups of cases may be aetiologically unrelated to M. faeni. One of six sera tested in this group reacted strongly with an extract of T. vulgaris. Antibodies to certain components of some extracts of M. faeni and of A. fumigatus were found in a high proportion of normal sera. Possible reasons for this finding have been given.


A modified test for Brucella agglutinins. 0.1% Protamine sulphate in normal (0.85% w/v) saline has been used as a diluent in Brucella serology instead of saline as such. The use of protamine sulphate in this concentration has obviated the need for performing the modified Coombs test to detect non-agglutinating Brucella antibodies. This method has been used in the Microbiological Diagnostic Unit of the University of Melbourne in the routine titration of 721 sera and has proved to give reliable and reproducible results.


Effect of medium composition on the apparent sensitivity of Pseudomonas aeruginosa to gentamicin. The diameter of inhibition zones in cultures of Ps. aeruginosa produced by discs of gentamicin varied on different media, as did the minimum inhibitory concentration (MIC) when the antibiotic was included in the medium. These variations, which were small in parallel tests of Esch. coli, were found to depend on the general composition of the medium, but were also affected by the agar used to solidify it. An agar on which the MIC was low had a low magnesium content, and two on which it was high had a high content. The addition of magnesium sulphate in increasing amounts to a medium of low content progressively increased the MIC. It thus appears that a high magnesium content, which is necessary for pyocyanin production, also increases the resistance of this organism to gentamicin.The difficulty of determining the sensitivity of Ps. aeruginosa to gentamicin as a guide to treatment is emphasized. The best method appears to be direct comparison with another strain of the same organism of known sensitivity.


Demonstration of group- and species-specific antigens of chlamydial agents by gel diffusion.


Neisseria meningitidis, a respiratory pathogen?


A longitudinal study of serum alpha-1-antitrypsin, alpha-2-macroglobulin, transferrin, immunoglobulins IgG, IgA and IgM, and H- and O-agglutinin titers in children following infection with Salmonella enteritidis.


Genetic analysis of a mutant of Bacillus subtilis producingltraviolet-sensitive spores.


Inactivation and mutagenesis on isolated DNA. V. The importance of repairing enzymes for the inactivation of transforming DNA in vitro.


Early discharge of tuberculous patients.


The fate of lipofuscin produced in the injured liver.


Relation of EEG findings in children with cerebral palsy to overall reaction on rehabilitation.


ST-feline fibrosarcoma virus: induction of tumors in marmoset monkeys. Two newborn marmosets, inoculated with a cell-free extract of feline fibrosarcomas, developed multiple sarcomas and died within 46 days of inoculation, whereas two of these animals inoculated with a crude homogenate developed no tumors. This susceptibility to a mammalian RNA sarcoma virus suggests that marmosets may be particularly suitable for attempts to isolate infectious agents from man.


Serum protein concentrations in pregnancy. I. Concentrations in maternal serum.


Heat-stabile alkaline phosphatase. A parameter of placental function.


The comparative anatomical study of the masseter of the mammal (3).


Long-term effect of an intrauterine contraceptive device on genital organs of rhesus monkeys. A 4 and one-half year study. To determine the long-term effect of an intrauterine device on the physiology and biochemistry of the uterus and Fallopian tubes, 4 rhesus monkeys fitted with the Margulis spiral IUD were followed for 4 1/2 years and compared with  sham-operated controls through biochemical, histological, and histochemical studies.  Results of the studies revealed no alteration of the menstrual cycle or gonadotropin activity.  Protein synthesis as measured by the incorporation of radioactive valine into uterine protein was also unaltered, indicating no aberrant tissue growth in the presence of the IUD.  There was a significant consistent increase in the level of calcium (20.8% over the controls' increase in the proliferative phase and 32.7% over in the secretory phase) and stimulation of acid phosphatase activity (62.5% rise in activity in  the secretory phase over the proliferative phases in IUD animals as compared with a 32.3% rise between the phases in the controls) in the uterus and Fallopian tubes of the monkeys with IUDs.  It is suggested that this may be due to continuing tissue reaction to a foreign object.  Long-term effects of the IUD on the histology and biochemistry of the genitals appear to be minimal, but further research is indicated.


Measurement of cerebral hemispheric blood flow by intracarotid injection of hydrogen gas. Validation of the method in the monkey.


Effect of absorption of IgG with insolubilized antiserum on reaginic activity of DEAE-serum fractions.


The evaluation of anticancer drugs in dogs and monkeys for the prediction of qualitative toxicities in man.


Tolerance to the vascular effects of glyceryl trinitrate.


Pulmonary arterial design in mammals. Morphologic variation and physiologic constancy.


Viral hepatitis in subhuman primates and its relationship to human viral hepatitis.


Disseminated pulmonary ossification. A case report with demonstration of electron-microscopic features.


Studies on synthesis of coumarin derivatives. 23. Synthesis and antibacterial activity of derivatives of 3-substituted-7-amino-4-hydroxy-coumarin.


Current concepts of cutaneous porphyria and its treatment with particular reference to the use of sodium calciumedetate.


Multiple electrophoretic forms of carbonic anhydrase in red cells of domestic cattle (Bos taurus) and American buffalo (Bison bison).


The antigenic properties of alkaline phosphatase of human kidney, kidney tumors and urine.


Quantitative aspects of the antibody response of rabbits to bacteriophage OX174.


Meningococcal and ECHO-9 meningitis. Report of an outbreak.


Conservation of the structure of ribosomal RNA during evolution.


Patterns of Marchi degeneration in the monkey pyramidal tract following small discrete cortical lesions.


Baseline rail-running performance in the squirrel monkey.


Bacteriological findings in positive tuberculin reactors in childhood.


The James Mackenzie Lecture.

The James Mackenzie Lecture.


Physicians' views of medical practice in nonmetropolitan communities.


Varying psychological sequelae of lead ingestion in children.


Approaches to primary prevention of disease.


Associations of cardiovascular mortality with weather in Memphis, Tennessee.


Vision screening of preschool children in mobile clinics in Iowa.


Outbreak of food poisoning caused by Bacillus cereus.


Analysis of mobility and change in a longitudinal sample.


Occurrence of biologic false positive reactions with RPR (circle) card test on leprosy patients.


Aseptic meningitis epidemic involving ECHO 4 and Coxsackie B5 viruses.


Children's perceptions of vulnerability to illness and accidents.


Suicides and homicides among Indians.

Suicides and homicides among Indians.


Changing patterns of hospitalization for psychiatric disorders.


Cerebral hemorrhage in relation to birth asphyxia. The brains of monke guinea pigs asphyxiated at birth pletely resuscitated, and killed a ous times thereafter revealed no chial hemorrhages. However, postnatal distress and other factoring to a moribund state occurro brains revealed petechial hemor.


Monosodium glutamate: specific brain lesion questioned.


The role of RNA as a growth factor for the cultivation of M. tuberculosis from patients after intensive and prolonged chemotherapy.


Precipitating antibodies to E. coli O antigens: a suggested difference in the antibody response of infants and children with first and recurrent attacks of pyelonephritis.


Fluorescent treponemal antibodies in fractionated syphilitic sera. The immunoglobulin class.


Effects of synthetic penicillin and cephalosporin on thyroxine-plasma protein interaction in the rat.


Physical and chemical properties of a protein isolated from red cell membranes.


Isolation of two lipolytic pituitary peptides.


Mammalian histidine decarboxylase. Activation of the apoenzyme with phospho-5'-pyridoximinotriazole.


Genetic analysis of the developmental processes during germination and outgrowth of Bacillus subtilis spores with temperature-sensitive mutants.


Passive sensitization of tissue cells. 3. A primate macrophage-cytophilic antibody.


Streptococcal anti-group A precipitins in sera from patients with rheumatic arthritis and acute glomerulonephritis.


The measurement of pseudofacility and true facility by constant pressure perfusion in the normal rhesus monkey eye.


Sensitivity of Gram-negative bacilli to ampicillin after six years' clinical use. A total of 1,102 clinical isolates of Gram-negative bacilli was obtained from four hospitals during 1967 and these cultures were tested for sensitivity to ampicillin. Approximately 80% of the strains of Escherichia coli and 90% of the strains of Proteus mirabilis, the two organisms most frequently isolated, were sensitive to ampicillin. Klebsiella-Enterobacter species and Pseudomonas aeruginosa were generally insensitive. Comparison of these results with data obtained in an earlier study with Gram-negative organisms isolated in 1961 showed that there had been no significant increase in the incidence of resistance of Gram-negative bacilli to ampicillin during the period 1961-67. The majority of ampicillin-resistant strains of E. coli isolated in 1967 transferred ampicillin resistance to a sensitive strain of E. coli K12. Only four ampicillin-resistant strains of E. coli isolated in 1961 were available for transferable resistance tests but all four strains transferred ampicillin resistance. Infective or transferable resistance was therefore a feature of ampicillin resistance of certain Gram-negative bacteria before ampicillin became generally available for clinical use.


Effect of drugs on vitamin B 12 levels obtained using the Lactobacillus leichmanii method. In 750 consecutive assays of serum vitamin B(12) levels using Lactobacillus leichmanii, 25 (3.3%) showed inhibition. A satisfactory history of drug therapy was obtained in 23 cases. Seventeen of these were receiving ampicillin at the time. Two patients showed inhibition while receiving phenoxmethyl penicillin or phenethicillin. A prospective study of 11 patients detected inhibition in seven at varying intervals during ampicillin therapy. It was shown that the vitamin B(12) as measured by the radioisotope and Euglena gracilis methods was not affected. Inhibition of L. leichmanii was reproduced in vitro with concentrations of ampicillin corresponding to those obtained in therapy. Benzylpenicillin, streptomycin, and chloramphenicol had no such effect.A prospective study of patients receiving chlorpromazine yielded negative results.Inhibition may occasionally be seen in the absence of any known intake of drugs. Measurement by other methods may be required to distinguish this from vitamin B(12) depletion.


Laboratory tests in viral hepatitis.

Laboratory tests in viral hepatitis.


Antibacterial action of combinations of colistin and the sulphonamides.


The lysis of cholera and El Tor Vibrios.


The fine structure of the protonephridia in the rotifer Asplanchna.


A new observation on human intraductal pancreatic pressure.


Prophylactic use of antibiotics.

Prophylactic use of antibiotics.


Newer antibiotics and hospital-acquired infections.


Dicloxacillin therapy of infections by gram-positive organisms.


Small doses of calcium edetate in the treatment of experimental and clinical lead poisoning.


Double blind trial of naphthypramide (DA-992) in osteoarthritis.


Overweight relapse: effects of training and methamphetamine with pentobarbital.


Effects of diphenylhydantoin (Dilantin) withdrawal on non-epileptics: preliminary report.


N-(1,1-dihydroperfluorooctyl)pyridinium trifluoromethanesulfonate, a new quaternary ammonium antiseptic.


Complexes of ergot alkaloids and derivatives. II. Interaction of dihydroergotoxine with certain xanthines.


Improved thin-layer chromatographic identification of tetracyclines and their degradation products: application to an epimerization study.


Potentiating effect of organomercurials on bisphenols.


Thresholds of laser eye hazards.

Thresholds of laser eye hazards.


US Air Force permissible exposure levels for laser irradiation.


Ocular damage thresholds for the helium-neon laser.


The specificity of proteinases from Streptomyces griseus (pronase). Purification of pronase by ion-exchange chromatography gave four proteolytically active fractions. Fraction A(2) contained an endopeptidase that attacks poly l-valine. Fraction B contained an endopeptidase, an aminopeptidase and carboxypeptidases. The activities against hippuryl-l-arginine and hippuryl-l-phenylalanine could be inhibited to a considerable extent by di-isopropyl phosphorofluoridate and by EDTA. Fraction C contained an endopeptidase resembling bovine trypsin. The pure enzyme was completely inactivated by di-isopropyl phosphorofluoridate and pancreatic trypsin inhibitor and to about 90% by other naturally occurring trypsin inhibitors. Fraction D contained an apparently homogeneous endopeptidase, inhibited by diisopropyl phosphorofluoridate, that adsorbed to and hydrolysed elastin. The activity of all these fractions was tested qualitatively against a wide range of small peptides and synthetic substrates.


Thymineless death in Lactobacillus acidophilus R-26.


Comparative studies on the haemolytic and Treponema pallidum immobilizing complement activity in the serum of different species.


Effect of LSD-25 on mitotic and meiotic chromosomes of mice and monkeys.


Origin and molecular size of immunoglobulin-A in the mesenteric lymph of the dog.


Reagin-like antibody formation in rabbits during a heterogeneous antibody response to Ascaris antigens.


Phylogenetic insight into evolution of mammalian Fc fragment of gamma G globulin using staphylococcal protein A.


The immunochemistry of immune tolerance. II. The relationship of chemical structure to the induction of immune tolerance to catechols.


Human M-proteins with antibody activity for nitrophenyl ligands.


Chemical modification of human and rabbit antistaphylococcal gamma G antibodies: effect of acetylation, carbamylation and amidination on opsonic activity.


Cellular kinetics of the antibody response by the fetal rhesus monkey.


Experimental hemorrhagic pneumonitis produced by heterologous anti-lung antibody.


Spontaneous thyroiditis in the obese strain of chickens. 3. The effect of bursectomy on the development of the disease.


Antibody responses in serum and nasal secretions according to age of recipient and method of administration of A2-Hong Kong-68 inactivated influenza virus vaccine.


Complications in brachial countercurrent arteriography and in retrograde femoral arteriography.


Bacteriologic studies on wasting disease induced by neonatal thymectomy.


Therapeutic implications of drug metabolism.


The histology of the endocrine pancreas and the insulin content in the microdissected islets of foetal pancreas.


Infrared absorption angiography of the fundus circulation.


Fundus cinephotography during gravitational stress.


Biochemical and physiological properties of an extracellular protease produced by Bacillus megaterium.


Cytosiderosis of the central nervous system in Parkinson's disease.


The molecular determinants of the A11 and A12 allotypic specificities in rabbit immunoglobulin.


The camouflage of Triatoma dimidiata and the epidemiology of Chagas' disease in Costa Rica.


Studies with Bay 2502 on animals.

Studies with Bay 2502 on animals.


Effect of 2,4-dinitrophenol on the phosphorylases system of the skeletal muscle in vivo.


Production of antibody for rat tail tendon collagen.


Blood and serum group typings in Koreans.


Growth and toxin synthesis in batch and chemostat cultures of Corynebacterium diphtheriae.


Aspects of membrane synthesis during the cell cycle of Bacillus megaterium and Escherichia coli.


Mucopeptide synthesis by protoplast membrane preparations of Bacillus megaterium.


Streptococcal taxonomy.

Streptococcal taxonomy.


Structural sites of RNA synthesis in Escherichia coli.


Irregular antibodies in the obstetric patient.


Lathyrism.

Lathyrism.


A histologic study of the changes in tissues of the periodontium of the Rhesus monkey with the application of torque force in the third stage of the Begg technique.


Experimental spinal cord angiography.

Experimental spinal cord angiography.


The use of oral Metoclopramide in the barium meal and follow-through examination.


The treatment of stage 3 carcinoma of the cervix by external radiotherapy and high-pressure oxygen.


Eye protection in cobalt 60 teletherapy beams.


Rickettsial antibodies in multiple sclerosis. The serum of subjects with multiple sclerosis or other degenerative neurological diseases and that of normal controls does not differ significantly in its content of antibodies to several rickettsial antigens. There is no evidence for a rickettsial aetiology of multiple sclerosis, and no grounds for an extended clinical trial of Le Gac's full method of treatment with antibiotics.


Septic shock.

Septic shock.


The effect of free fatty acids on resin uptake of tri-iodothyronine from human, rat, rabbit and guinea-pig serum and human serum albumin.


Nerve fiber growth and myelination in Von Recklinghausen eighth nerve tumor tissue.


Serological classification of the genus Sporobolomyces.


Fatty acids in the tooth lipids of 16 animal species.


To make a dent in the world TB problem--the TB program must be inexpensive, simple, and country-wide.


Professional observations in North America.


[8-Arginine]-vasopressinoic acid: an inhibitor of rabbit kidney adenyl cyclase. Neurohypophyseal hormnones and several synthetic analogs stimulate adenyl cyclase prepared from rabbit kidney medullary tissue. [8-Arginine]-vasopressinoic acid inhibits the stimulation of medullary adenyl cyclase by neurohypophyseal peptides but does not influence the action of parathyroid hormone on adenyl cyclase from kidiney cortex.


Tissue response to x-irradiated tooth transplants.


Rate of water and sodium absorption from the jejunum after abdominal surgery in man.


Autosomal recessive oculocutaneous albinism in man. Evidence for genetic heterogeneity.


Positive nitrogen balance immediately after abdominal operations.


Surgical management of diverticulum of the canaliculus.


Ultrastructure of the optic nerve head.

Ultrastructure of the optic nerve head.


A particular form of muscular inhibition in epilepsy: the related epileptic silent period.


The anterior lobe and the lingula of the cerebellum in monkeys and man.


Effects of freezing on peripheral leucocytes.


Gram-negative bacilli on the skin.

Gram-negative bacilli on the skin.


A study on the antigen G in the Rh system.


Methods for studying the epidemiology of Pseudomonas aeruginosa in the hospital environment.


The hemoglobins of Artemia salina. I. Determination of phenotype by genotype and environment.


Comparative studies of mammalian adenosine deaminases--some distinctive properties in higher mammals.


Lysozyme activity in baboon milk.

Lysozyme activity in baboon milk.


Periarticular ectopic ossification in hemiplegics.


The mosaic of personal relationships in geriatrics.


Bacteria, bile, and the small bowel.

Bacteria, bile, and the small bowel. Microbial populations of the small bowel and bile salt metabolism were studied in 15 patients with lesions of the stomach and small intestine. These types of microorganism could be correlated with the site and extent of stasis in the small bowel and the presence of a normally functioning stomach. The presence of obligate anaerobes (bacteroides) and free bile acids could be correlated with areas of stagnation. When these abnormalities were detected throughout the small bowel, steatorrhoea was also noted. However, bacteroides and free bile acids in localized regions of either proximal or distal small bowel were generally associated with normal faecal fat excretion. Vitamin B(12) malabsorption appeared to be related to the total number of bacteria colonizing the small bowel rather than to any specific type of microorganisms. The effect of antibiotics on intestinal function and bacteriology was studied in three patients. In one patient, the broad-spectrum antibiotic tetracycline was effective in eradicating an abnormal bacterial flora. In the other two, lincomycin, which is specifically effective in eradicating the anaerobic flora, restored intestinal function to normal.


Long-term care: the nursing home industry. 1.


Partial purification and characterization of dihydrodipicolinic acid synthetase from sporulating Bacillus megaterium. Sporulation of Bacillus megaterium Km (ATCC 13632) was synchronized by a technique employing three 10% transfers. The culture was harvested when 60% of the cells contained spore forms. Dihydrodipicolinic acid synthetase was purified 150-fold by ammonium sulfate fractionation at pH 6.5, heating for 15 min at 45 C at pH 6.0, ammonium sulfate fractionation at pH 6.0, and subsequent chromatography on diethylaminoethyl cellulose. During the final stage of the purification procedure, the enzyme exhibited sensitivity to refrigeration temperatures. The enzyme had a pH optimum of 7.65 in imidazole buffer. The apparent K(m) values were 4.6 x 10(-4) and 5.0 x 10(-4)m for beta-aspartyl semialdehyde and pyruvate, respectively. All attempts to demonstrate cofactor requirements were unsuccessful. Sulfhydryl inhibiting reagents and lysine did not inhibit the enzymatic reaction. The enzyme exhibited maximal thermal resistance at pH 10.5. The thermal stability of the enzyme at 75 C was increased more than 1,800-fold by the addition of 0.3 m pyruvate. The E(a) was 67,300 cal/mole for the thermal denaturation of the enzyme. At 60 C, the DeltaF, DeltaH, and DeltaS values for the thermal denaturation of the enzyme were 22,250, 66,700, and 133 cal per mole per degree, respectively.


Stabilizing effect of colicin E2 on the "spheroplast membrane". Cells of Escherichia coli W3110 and its thymineless mutant, both of which are colicin E2 sensitive, were treated with colicin E2, and then converted to spheroplasts. These spheroplasts seemed to be more stable than those from untreated cells; suspensions of spheroplasts of untreated cells were lysed spontaneously and the turbidity was reduced by approximately 45% on incubation with ethylenediaminetetraacetic acid-lysozyme, whereas suspensions of spheroplasts of colicin E2-treated cells showed 25% reduction in turbidity. This change was irreversible and 5 min of treatment with colicin E2 at 37 C was necessary for stabilization. This process was inhibited by 2,4-dinitrophenol or streptomycin. Cells harboring the colicin E2 factor were not affected by treatment in this way with colicin E2. Alteration of composition of phospholipids was not observed.


Catabolic activities of Neisseria meningitidis: utilization of glutamate. Glutamate was catabolized at a rapid rate by Neisseria meningitidis, group B. Surprisingly, there was a lag of 5 to 30 min in respiration, but not in CO(2) production from C(1), and an appreciable amount of succinate accumulated. The eventual rapid rate of respiration was not prevented by the addition of chloramphenicol. The lag period was eliminated by combinations of substrates that favored the activity of a glutamate-oxaloacetate transaminase. It is suggested that with glutamate as the sole substrate, the reaction terminated at succinate, required only moderate O(2) uptake, and did not result in the transport of succinate to enzymatic sites. The lag period represented the time required for the accumulation of succinate and its transport to enzymatic sites by energy provided by the metabolism of the remaining glutamate. When the transaminase was operative, on the other hand, successive products of the reaction were immediately placed in contact with enzymatic sites.


Extrusion of sodium and hydrogen ions as the primary process in potassium ion accumulation by Streptococcus faecalis. Glycolyzing cells of Streptococcus faecalis accumulate K(+) with concurrent extrusion of equivalent amounts of H(+) and Na(+). An attempt was made to clarify the retionship between the movements of Na(+) and K(+). Sodium was displaced from cells glycolyzing in the presence of ammonia, diethylamine, tris(hydroxymethyl)aminomethane, and other nitrogenous cations; by contrast, K(+) was completely retained. Accumulation of K(+) by heterologous exchange for Na(+) was not inhibited by antibiotics which facilitate diffusion of K(+) across the membrane, but was blocked by proton conductors. The results indicate that extrusion of Na(+) and H(+) from the cells is a primary, energy-linked process which generates an electrical potential (interior negative); K(+) accumulation occurs in response to this potential. Two mutants deficient in K(+) accumulation and retention were examined in terms of this model. One mutant is apparently defective in exchange of K(+) for H(+). In the other mutant, exchange of K(+) for Na(+) is impaired.


Incorporation of 5-methyl- and 5-hydroxy-tryptophan into the protein of Bacillus subtilis. Addition of the tryptophan analogues 5-methyl-tryptophan, 5-hydroxy-tryptophan, and 7-aza-tryptophan, during tryptophan starvation of a tryptophan auxotroph of Bacillus subtilis, stimulated amino acid incorporation. Two of the analogues tested, 5-methyl-tryptophan and 5-hydroxy-tryptophan, competed with each other, but their addition did not prevent tryptophan incorporation into acid-insoluble material. The incorporation of tryptophan and 5-hydroxy-tryptophan was stimulated when a mixture of phenylalanine and tyrosine was present in the medium. The two analogues 5-methyl-tryptophan and 5-hydroxy-tryptophan were recovered in protein hydrolysates of analogue-grown cultures, and there is evidence suggesting that the analogues are not chain terminators. Neither analogue prevented the polysome degradation that occurs during tryptophan starvation.


Regulation of penicillinase synthesis: evidence for a unified model. The kinetics of penicillinase induction in Bacillus cereus 569 was investigated. An increase in the rate of penicillinase synthesis was demonstrated within 30 sec of the addition of inducer (benzylpenicillin); however, the maximum induced rate of penicillinase synthesis was not attained until at least 30 min after the addition of inducer. In contrast to earlier claims, a quantitative estimate showed that the penicillinase messenger ribonucleic acid (mRNA) half-life is approximately 2 min. These findings strongly suggest that the rate of synthesis of penicillinase mRNA increases continuously during most of the 30-min latent period. A model for the regulation of penicillinase synthesis in three gram-positive organisms is presented which is consistent with a nondiffusible inducer, a short-lived mRNA, a relatively long latent period (i.e., an apparently slow inactivation of penicillinase repressor), and the existence of at least two regulatory genes.


Repression of phenolic acid-synthesizing enzymes and its relation to iron uptake in Bacillus subtilis. Excretion of the metal-chelating phenolic acid, 2,3-dihydroxybenzoate, by a tryptophan-requiring strain (M-13) of Bacillus subtilis was inversely proportional to the iron added to the medium. Addition of iron as the ferric chelates of two secondary hydroxamates (ferri-schizokinen and Desferal) markedly reduced excretion. Synthesis of 2,3-dihydroxybenzoate from chorismate by extracts of B. subtilis M-13, grown in low-iron medium, was unaltered by additions of FeSO(4), FeCl(3), ferrischizokinen, 2,3-dihydroxybenzoate, the 2,3-dihydroxybenzoate-iron complex, or by extracts of cells grown in high-iron medium (which contained no demonstrable 2,3-dihydroxybenzoate-synthesizing activity) to the extracts of "low-iron cells." Iron control seemed to involve repression of synthesis of the enzymes in the 2,3-dihydroxybenzoate pathway. Both ferri-schizokinen and 2,3-dihydroxybenzoate plus iron enhanced considerably the otherwise minimal repressive effects of iron at low concentrations. Ferri-schizokinen delayed derepression of the pathway in B. subtilis M-13, and reduced its rate of synthesis after derepression. Addition of FeSO(4) to derepressed cells of B. subtilis M-13 halted synthesis of the enzymes after a lag period. The effect of the ferric hydroxamates was related to the capacity of B. subtilis M-13 to incorporate (59)Fe(3+) from Desferal-(59)Fe(3+). Cellular accumulation of (59)Fe(3+) from Desferal-(59)Fe(3+) after 20 min was nearly double that incorporated from (59)FeCl(3).


Chemical composition of exosporium from spores of Bacillus cereus. Homogeneous fragments of exosporium were extricated in centigram amounts from dormant spores of Bacillus cereus and analyzed for intrinsic constituents. The membrane proved to be chemically complex but not unique, consisting mainly of protein (52%), amino and neutral polysaccharides (20%), lipids (18%), and ash (4%). Seventeen common amino acids were identified by chromatography, and were present in usual proportions except for low levels of cystine-cysteine, methionine, tyrosine, and histidine. Glucosamine was the only amino sugar, and glucose and rhamnose were the principal neutral sugars. The lipid fraction contained 5.5% cardiolipin as the only phospholipid, 12.5% neutral lipids, and at least 19 fatty acids, among which normal C(16) and C(18) ones predominated. Calcium and phosphorus occurred in the ash. Small amounts of teichoic, ribonucleic, and dipicolinic acids were believed to represent contamination.


Macromolecular synthesis during microcycle sporogenesis of Bacillus cereus T. Microcycle sporogenesis induced in Bacillus cereus T by phosphate limitation occurs over a narrow range of phosphate to spore inoculum ratios. Sufficient phosphate is required to satisfy the demands for a twofold increase in deoxyribonucleic acid; net ribonucleic acid synthesis is not required. The total ribonucleic acid content of the culture was variable, and deoxyribonucleic acid synthesis was restricted to a twofold increase. Developmental changes during outgrowth occurred synchronously, whereas enzyme synthesis was periodic. The timing of the synthesis of tricarboxylic cycle enzymes, extracellular protease, arginase, histidase, and alkaline phosphatase was measured. Histidase could be induced after 2.5 hr throughout microcycle sporogenesis. Several other features of macromolecular synthesis during microcycle sporogenesis are described. Differences between this pattern and those observed during outgrowth leading to cell division are discussed. A technique for accurately estimating the levels and time of synthesis of incompletely extractable, labile enzymes is also presented.


Replacement sporulation of Bacillus subtilis 168 in a chemically defined medium. A replacement sporulation technique (i.e., the sporulation of vegetative cells upon suspension in an appropriate medium) has been developed for Bacillus subtilis 168 (a transformable Marburg strain of B. subtilis). The replacement sporulation medium used is composed of inorganic salts and 10 mm ammonium lactate or glutamate. The requirement for ammonium lactate or glutamate could also be satisfied by other compounds that are metabolized via the tricarboxylic acid cycle. Sporulation of the suspended vegetative cells was completed by 8 to 10 hr after suspension, and the resulting spores were indistinguishable from spores produced in a conventional growth and sporulation medium. Various physiological changes previously reported to be associated with sporulation (e.g., increase in the level of tricarboxylic acid cycle enzymes and changes in the rates of synthesis of deoxyribonucleic acid, ribonucleic acid, and protein) could also be demonstrated during replacement sporulation.


Resistance of Escherichia coli to penicillins. VI. Purification and characterization of the chromosomally mediated penicillinase present in ampA-containing strains. The chromosomally mediated penicillinase present in three strains of Escherichia coli K-12 has been purified and characterized. Two of the strains carried the ampA gene and the third the wild-type allele. The purification involves release of the enzyme by spheroplast formation, dialysis, chromatography on sulfoethyl cellulose, and chromatography on hydroxylapatite. Enzyme from the two mutants appeared homogeneous in polyacrylamide gel electrophoresis. Enzyme from the wild-type strain gave two bands. Immunologically, the enzymes from all three strains were identical. Ultracentrifugation gave a homogeneous peak with a sedimentation coefficient of 3.4S. Gel filtration gave an estimated molecular weight of 29,000. The N-terminal amino acid residue was found to be alanine. Complete amino acid analysis showed a lack of cysteine. Ultraviolet spectra were recorded at three different pH values. The extinction coefficient at 280 nm is 21.0 for a 1% solution at pH 6.8. The optimal pH is 7.3. With enzyme from one of the resistant mutants, the following K(m) and turnover number values were obtained: for penicillin G, 12 mum and 2,080; for d-ampicillin, 6 mum and 83; for cephalosporin C, 217 mum and 18,400. The effect of different salts on the enzyme activity was tested. Under many conditions the enzyme was found to be unstable.


Effect of beta-lysin on isolated cell walls and protoplasts of Bacillus subtilis. The cytoplasmic membrane is the site of beta-lysin action. Protoplasts lysed rapidly in its presence, whereas cell walls and wall autolysis were unaffected.


Biochemical studies of bacterial sporulation and germination. XXI. Temperature-sensitive mutants for initiation of germination. Mutants of Bacillus megaterium have been isolated that are temperature-sensitive for the initiation of germination.


Incorporation of uridine into Bacillus subtilis and SPP1 bacteriophage deoxyribonucleic acid. Tritiated uridine is incorporated into the deoxyribonucleic acid of Bacillus subtilis and bacteriophage SPP1; the tritium is recovered in the cytidine moiety of both deoxyribonucleic acids.


Early energy-dependent step in the entry of transforming deoxyribonucleic acid. It was found that cyanide can partially reverse as well as arrest the transformation of a newly transformed deoxyribonuclease-treated culture for 6 to 8 min after the addition of the enzyme. These findings strongly suggest that deoxyribonucleic acid which attained deoxyribonuclease-insensitivity is not necessarily in the cell, but stored in some region peripheral to the membrane, and that a rate-limiting, energy-dependent step is required to transport this deoxyribonucleic acid into the cell.


Ankylosis in the paralyzed patient.

Ankylosis in the paralyzed patient.