Lactate dehydrogenase gene function in the blind cave fish, anoptichthys jordani, and other characins.


The child with Hurler's syndrome.

The child with Hurler's syndrome.


Experimental and clinical autogenous distal metacarpal reconstruction.


Ankylosing hyperostosis of the spine.

Ankylosing hyperostosis of the spine.


Comparative physical-chemical studies of mammalian arginases.


Specific inhibition of cell contact formation in Dictyostelium by univalent antibodies.


A comparative immunologic and electrophoretic analysis of rat and mouse caseins.


Actinomycin D binding to isolated deoxyribonucleoprotein and intact cells.


Selective extirpation of oral ciliature of Blepharisma by laser microbeam.


Fine structure of nuclei as revealed by electron microscopy. VII. Hyperplastic liver nodules in rat induced by 3'-methyl-4-dimethylaminoazobenzene, 2-fluorenylacetamide and DL-ethionine.


Familial hyperglobulinaemic purpura in two young siblings. Clinical and immunobiologic studies.


Human carbonic anhydrases. V. Levels in erythrocytes in various states.


Mandibular movements of the monkey.

Mandibular movements of the monkey.


Australia antigen in systemic lupus.

Australia antigen in systemic lupus.


Coccidiosis and toxoplasmosis.

Coccidiosis and toxoplasmosis.


Cost of gluten-free diet.

Cost of gluten-free diet.


Cranioskeletal displacements caused by rapid palatal expansion in the rhesus monkey.


Adaptive growth changes of the gonial region in Macaca mulatta.


Bipedal behavior and the emergence of erect posture in man.


Origin of the human hand.

Origin of the human hand.


Erythrocyte carbonic anhydrase activity in health and disease.


Clindamycin (Cleocin) in bacterial pneumonia.


A double-blind evaluation of the anti-emetic efficacy of benzquinamide, prochlorperazine and trimethobenzamide in office practice.


The treatment of contact dermatitis with AHR-1911, a new non-steroid anti-inflammatory agent.


Warfarin therapy not influenced by meprobamate. A controlled study in nine men.


Nicotinic acid as an adjuvant in the treatment of chronic schizophrenic patients with special reference to changes in higher nervous activity.


Human pharmacology of phentonium, a new autonomic drug.


Digitalis antagonism by a specific lactone.


A student exercise in clinical pharmacology: Renal effects of diuretics.


The ankle sprain.

The ankle sprain.


New horizons for intravenous feeding.

New horizons for intravenous feeding.


Fungi in the Lebanon sewage treatment plants and in Turtle Creek, Warren Co., Ohio.


Further environmental factors affecting the antigenicity of Trichophyton rubrum.


Immunochemical study of Mycobacteria firmly bound lipids.


A serological comparison of Phialophora verrucosa, Fonsecaea pedrosoi and Cladosporium carrionii using immunodiffusion and immunoelectrophoresis.


Latex slide agglutination test for Aspergillus antibodies.


Therapeutic observations with Morfolep in various types of epilepsy.


Some observations on brucellosis in a Jersey herd, 1965-1969.


The concept of Fourier transform spectroscopy.


A comparative Golgi study of Cajal foetal cells.


Fluorescence microscope study of a colourless euglenoid Cyclidiopsis acus.


Histoenzymology of frog adrenal beta-glucuronidase: a histochemical model for hormone-enzyme relation.


Histochemical changes in the testes of heat-exposed toad.


Comparative study of morphological methods used for the determination of calcium salts.


Comparative study of human allergic antibodies in prausnitz-küstner test and in passive sensitization of isolated human smooth muscle organs.


Studies on vaccination against bacillary dysentery. 5. Studies in Erythrocebus patas. In earlier studies in man it has been demonstrated that streptomycin-dependent shigellae, if given orally in 5 sufficiently large doses, confer a very high type-specific protection against bacillary dysentery.In the present study, 2 groups of Erythrocebus patas monkeys were immunized with live streptomycin-dependent Shigella flexneri 2a, and a third group was not vaccinated. One of the vaccinated groups was given streptomycin with each dose of the live vaccine. The animals that received streptomycin with the vaccine were shedding these organisms in their faeces for a significantly longer period than the animals that did not receive streptomycin. A week after the last dose of vaccine, the animals were challenged with virulent Sh. flexneri 2a organisms. All the control animals developed diarrhoea and excreted challenge organisms for an average of 9 days. None of the 9 immunized animals showed pathological changes nor symptoms of dysentery. On average, they excreted challenge organisms for only 2.3 days.Immunological tests confirmed the immunity of the vaccinated animals.This study shows that 3 doses of live oral streptomycin-dependent Shigella vaccine given together with streptomycin are at least as effective as immunization with 5 doses of the same vaccine without streptomycin. Oral application of streptomycin, therefore, seems to have had an enhancing effect on the immunizing property of live oral streptomycin-dependent enteric vaccines.


A rapid quantitative ion-exchange extraction fr 2,8-dioxyadenine.


Cytology and histology of haemopoietic cell transplantation under the influence of antilymphocyte serum (ALS) in mice. I. The in vivo effect of antilymphocytic globulin on haemopoietic tissue.


Perseverative interference in monkeys following selective lesions of the inferior prefrontal convexity.


The effect of 1-phenylalanine mustard on anti-globulin antibodies in multiple myeloma.


Effect of feeding of acidophilus milk on intestinal lactobacilli and other gut microorganisms and on bile salt metabolism. Preliminary study.


Assessments of toxicity of substances.

Assessments of toxicity of substances.


Studies of rubella virus. 1. A study of growh of rubella viru in kidney culture cells of African green monkey.


Dimerization mutants of alcohol dehydrogenase of maize. Two ethyl methanesulfonate-induced mutants of the alcohol-dehydrogenase gene in maize have been analyzed. The enzyme subunits produced by the mutants seem to be structurally altered in such a way that the subunit specified by the null allele Adh(1) (0(5657)) does not dimerize with "wild-type" subunits, and that specified by Adh(1) (S(296)) does so only to a limited extent.


Nuclear involvement poin xvirus infection. During the first hours of infection with vaccinia virus, part of the chromosomal DNA of host cells is degraded, part of it is released from the cell, and part of it eventually appears in cytoplasmic foci associated with the reproduction of virus. The DNA of the infecting particle is first found attached to the chromosomes. Intranuclear foci of DNA incorporation appear later; these rapidly move out into the perinuclear space and eventually become typical cytoplasmic "virus factories." Each of these observations points to a more direct involvement of the nucleus in viral infection than was previously envisaged.


Staphylococcal clumping and fibrinogen and fibrin degradation products in inflammatory exudate.


DNA polymerase activities in varions of visna virus, a causative agent of a "slow" neurological disease. The presence is reported of an RNA-instructed DNA polymerase in visna virus, the causative agent of a "slow" neurological disease in sheep. The product synthesized by the RNA-directed reaction has been shown to be a DNA heteropolymer by the following criteria: synthesis requires the presence of all four deoxyriboside triphosphates; the product is resistant to ribonuclease and alkali but is degraded by DNase; and the product has a density of 1.420 in Cs(2)SO(4) solution, characteristic of DNA.Visna virions, like those of the oncogenic RNA viruses, contain DNA polymerase activities that respond to a variety of double-stranded DNAs and to synthetic DNA.RNA hybrids.


Food intake and temperature stress in the monkey.


Metabolic effects of nitroglycerin and tranylcypromine in unanesthetized rabbits.


Australia (hepatitis-associated) antigen: physicochemical and immunological characteristics.


A study of the causes of failure of esophagocardiomyotomy for achalasia.


Combined parenteral hyperalimentation and chemotherapy in the treatment of disseminated solid tumors.


Use of a chemically defined, liquid, elemental diet for nutritional management of fistulas of the alimentary tract.


Survival of Listeria monocytogenes in experimentally infected mice. Physiological saline was found to be very detrimental to the viability of Listeria monocytogenes. The LD(50) value was substantially reduced when peptone was used as the suspending fluid rather than saline. Normal splenic tissue was not inhibitory to the survival of Listeria. In experimentally infected mice, L. monocytogenes survived for 8 days in the peritoneal cavity and for at least 11 days in the spleen.


Rapid method for the isolation of Listeria monocytogenes from experimentally infected mice. Listeria monocytogenes was successfully isolated from experimentally infected mice by placing homogenized tissues in phosphate-buffered saline (PBS), trypsin, peptone, or pepsin followed by incubation at 37 C for 24 hr. A larger number of Listeria isolates were recovered from the trypsin or PBS splenic homogenate suspensions incubated at 37 C for 2 hr than from the other diluent suspensions. Holding infected tissues at 4 C for at least 3 months did not increase the efficiency of Listeria isolation. Listeria L-forms were not isolated from mice injected with the bacterial form. The in vitro viability of Listeria L-forms suspended in PBS or PBS splenic homogenate was greatly reduced when held at 4 C.


Biochemical alterations in Bacillus megaterium as produced by aflatoxin B1. Bacillus megaterium NRRL B-1368 cells and spores were produced on Trypticase Soy Broth (TSB) and Agar (TSA) containing 3.8 mug of aflatoxin B(1) per ml, analyzed for selected chemical constituents, and compared to cells and spores of B. megaterium produced on nontoxic Trypticase Soy Media. There was an initial 30% kill of cells after inoculation into toxic TSB and during the first 3.5 hr of incubation followed by a logarithmic growth phase in which the generation time was 75 min as compared to 20 min for the control culture. Chemical analyses revealed an increase in protein, deoxyribonucleic acid (DNA), and ribonucleic acid (RNA) on both a per cell basis and a per cent dry weight basis when B. megaterium was grown in toxic TSB. There was a concurrent decrease in the total amounts of cellular protein, DNA, and RNA synthesized in toxic TSB. Amino acid analyses of control and test cell walls showed little, if any, difference in cell wall composition. About 97% sporulation of B. megaterium occurred after 3 days on nontoxic TSA although 6 days were required to attain 65% sporulation on toxic TSA. Germination of spores was not inhibited by 4.0 mug of aflatoxin per ml but outgrowth was. No significant differences were observed in the heat resistance, protein, DNA, RNA, or dipicolinic acid content of spores formed on toxic TSA and nontoxic TSA.


Morphological alterations in Bacillus megaterium as produced by aflatoxin B1. Morphologically abnormal cells were produced by Bacillus megaterium NRRL B-1368 in response to aflatoxin B(1). Filamentous forms were characterized by early granulation and unusually large and numerous deposits of poly-beta-hydroxybutyric acid within the cells. Pantoyl lactone was without effect as a reversing agent for the observed inhibition of cell septum formation. B. megaterium cells and spores produced on toxic (3.8 mug of aflatoxin B(1) per ml) and nontoxic Trypticase Soy Broth and Trypticase Soy Agar (TSA) were observed by using phase contrast and electron microscopy. Transfer of aberrant forms to nontoxic TSA yielded macrocolonies with daughter cells morphologically indistinguishable from untreated cells. Agar slide cultures of filamentous cells transferred to nontoxic TSA indicated that normal cells were formed. Electron photomicrographs showed a decreased number of mesosomes in filamentous cells as compared to control cells. There were no observable morphological differences in spores formed on toxic or nontoxic TSA.


Gel diffusion method for determining the titer of duck hepatititis virus. Apparatus necessary for a quantitative gel diffusion and precipitation assay of duck hepatitis virus was designed and constructed.


Agar diffusion method for the differentiation of Bacillus anthracis. A method was developed for identification of Bacillus anthracis based on elaboration of protective antigen by individual colonies and its detection by double-diffusion precipitation in agar plates.


Injury of bacteria by sanitizers.

Injury of bacteria by sanitizers. Injury of test cultures was quantitated by differences in colony counts obtained with a complete medium and those obtained on conventional selective media. Staphylococcus aureus, Streptococcus faecalis, and several strains of Escherichia coli were injured when exposed to the quaternary ammonium compound methylalkyltrimethyl ammonium chloride. Representative hypochlorite sanitizers also caused injury of E. coli ML30. Sanitizer concentration appeared to be the main factor in the cause of death and injury, a higher concentration being needed to cause death. Increases in temperature did not result in substantial increases in injury; however, the lethal effect was greater at higher temperatures. Varying the cell concentration from 10(7) to 10(9) cells per ml did not change the fraction of cell population killed or injured. The inability or failure of common selective media to detect injured bacteria in food could have serious public health consequences.


Semisynthetic penicillin 6-(D(--)-alpha-carboxy-3-thienylacetamido) penicillanic acid active against Pseudomonas in vitro. The activity of 6-[d(-)-alpha-carboxy-3-thienylacetamido] penicillanic acid, BRL2288, was determined against Pseudomonas aeruginosa and various gram-negative bacilli. The majority of Pseudomonas strains (89%) were inhibited by 100 mug of the antibiotic per ml. BRL2288 is twofold more active than carbenicillin against Pseudomonas at 100 mug/ml or less. Among Enterobacteriaceae tested, 87% Enterobacter and 87% of Proteus mirabilis strains were inhibited by 25 mug/ml or less. Indole-positive Proteus were inhibited by 10 mug/ml or less. Fifty-five per cent of ampicillin-resistant Escherichia coli were inhibited by 100 mug/ml. Klebsiella were uniformly resistant. BRL2288 is not hydrolyzed by most resistant Pseudomonas, but it is destroyed by the beta-lactamases of E. coli and P. mirabilis. The antibiotic shows synergy with gentamicin but not with penicillinase-resistant penicillins such as cloxacillin. Activity of BRL2288 against gram-positive organisms is two- to eightfold less than that of ampicillin or benzylpenicillin G.


Lipoprotein inhibitor of Newcastle disease virus from chicken lung. A lipoprotein inhibitor of Newcastle disease virus was obtained from chicken lung tissue by means of dilute alkaline extraction procedures. The inhibitor was further purified by ammonium sulfate fractionation, isoelectric precipitation, and density gradient centrifugation. The purified lipoprotein inhibited active Newcastle disease virus hemagglutination at a concentration of 2.0 mug/ml which represented a 30-fold purification over the original extract. Infection of chicken embryo fibroblasts by Newcastle disease virus was also inhibited by the purified lipoprotein, the degree of inhibition depending upon the inhibitor-to-virus ratio. Chemical analysis of the purified inhibitor provided a composition of 72% lipid, 26% protein, and 3% carbohydrate, although some compositional variation was observed from one preparation to another. The chloroform-soluble lipids were shown to contain 40 to 50% phospholipid and 10 to 20% cholesterol; of the fatty acids recovered from the saponified lipoprotein, 39% was palmitic, 22% oleic, and 17% stearic. Careful analyses of large quantities of the inhibitor revealed a small (0.84%) but significant content of sialic acid. Removal of sialic acid from the lipoprotein by means of digestion with neuraminidase produced a sharp diminution in inhibitory properties. A delipidized form of the inhibitor was obtained by ether extraction, and this material produced a single broad band of precipitate in gel immunodiffusion tests.


Immunosuppression and experimental virus infection of the nervous system.


Transplacental transmission of rubella virus infection in rabbits. Rabbits were inseminated artificially and inoculated 1 week later with rubella virus. The attenuated vaccine strain HPV-77 or strain 67-1127 which had been through 12 cell culture passages was used. Half of the rabbits in each group had been inoculated 1 month prior to insemination with the corresponding strain. Evidence of infection induced by either strain was obtained by immunofluorescence in lung, spleen, and placenta. Rubella virus antigen was also found in the lung of a 2-month-old rabbit of a dam inoculated with strain 67-1127. A high rate of stillbirths and neonatal deaths and poor weight gain occurred in litters of the 67-1127 group.


High-yield method for dispersing simian kidneys for cell cultures. A technique for dispersion of animal tissue cells is described. The proposed technique is based on the concomitant use of trypsin and disodium ethylenediamine tetraacetate (EDTA). The use of the two dispersing agents (trypsin and disodium EDTA) markedly enhances cell yield as compared with the standard cell dispersion methods. Moreover, significant reduction in the amount of time required for complete tissue dispersal, presence of a very low number of nonviable cells, less cell clumping, and more uniform monolayer formation upon cultivation compare favorably with the results usually obtained with the standard trypsinization technique.


Multiple genes for lysozyme in birds.

Multiple genes for lysozyme in birds.


Incorporation in vivo of 14C-labeled precursors into the proteins of rabbit uterine secretions.


Chronic meningococcemia.

Chronic meningococcemia.


Manifestations of late congenital syphilis. An analysis of 271 patients.


Low-temperature (4-77 degrees K) spectroscopy of Anacystis: temperature dependence of energy transfer efficiency.


Properties of the membrane-bound respiratory chain system of Halobacterium salinarium.


Hunter's syndrome. Gargoyles--Washington Irving--"Rip Van Winkle".


Isolation and some chemical properties of human factor VIII (antihemophilic factor).


Nonimmunoglobulin precipitin lines between intestinal fluids and antisera in immunodiffusion studies: an explanation for their occurrence and how they can be avoided.


Proteins in gastric juice: a study of fifty cases.


Aerobic cometabolism of DDT analogues by Hydrogenomonas sp.


Studies on the precipitinogenic pattern of mycobacteria cultivated at various temperatures.


Specific and nonspecific stimulation of resistance in mice aganist infection with Mycobacterium tuberculosis H37Rv.


Some temperature influences on mycobacteria.


The virulence of M. tuberculosis isolated from tuberculosis patients in Bahia.


Oral manifestations of systemic genetic disorders. II.


Chronic arthritis, vasculitis and cutaneous histoplasmosis.


Benign fibro-osseous lesions of the mandible and maxilla: clinical features.


Hydrogen-tritium exchange in human erythrocyte carbonic anhydrases B and C.


Effects of nitroglycerin on "maximal" oxygen intake and exercise electrocardiogram in coronary heart disease.


Appetite-stimulating and weight-gain properties of cyproheptadine) (Periactin) in geriatric subjects.


Some immunological properties of rabbit antiserum to mouse prolactin.


Effects of two end products on enzyme repression in purine nucleotide biosynthesis.


Iodination of erabutoxin b: diiodohistidine formation.


Difference spectrophotometric study of interaction between maltose and saccharifying alpha-amylase from Bacillus subtilis.


The effect of a changed environment on bacterial colonization rates in an established burns centre. In an established burns centre which moved from an old building to new purpose-designed premises, colonization rates of patients' burns with Staphylococcus aureus, Pseudomonas aeruginosa and other Gram-negative bacilli were not reduced. Colonization rates with Streptococcus pyogenes increased but the increase was mainly due to multiple importations in the new premises of a strain of higher communicability than any seen in the old.In the first 32 months in the new environment 10 patients were found colonized with pseudomonas on admission and 20 became colonized in the unit. A much higher proportion of patients with burns of more than 30% body surface became colonized than of patients with less. About one-third of the above 20 patients became colonized with strains already isolated from another patient; all but one of them had small area burns. Cross-infection was not observed from numerous heavily colonized patients with high percentage burns. This paradox is discussed in detail. Basin outflows in the new premises became colonized with P. aeruginosa of two serotypes not found on patients in this unit.


[Short history of diseases of the world. Food poisoning].


Nursing notes on long-stay care.

Nursing notes on long-stay care.


A technique for imaging patient treatment area during a therapeutic radiation exposure.


The pregnant Bantu diabetic.

The pregnant Bantu diabetic.


Inapparent maternal infection by Coxiella burnetii and fetal repercussions.


Gene dosage for 5S ribosomal ribonucleic acid in Escherichia coli ad Bacillus megaterium. The number of gene copies for 5S ribosomal ribonucleic acid (rRNA), relative to that for 16 and 23S rRNA, has been determined by deoxyribonucleic acid (DNA)-RNA hybridization for Escherichia coli and Bacillus megaterium. In both cases, the number of 5S rRNA genes equals the number of 16 or 23S rRNA genes. Rapid procedures for preparing extremely highly purified DNA suitable for DNA-RNA hybridization experiments and chemically pure 5S rRNA are described.


Evidence for a magnesium pump in Bacillus cereus T. Unlike Escherichia coli, Bacillus cereus T appears to accumulate Mg(2+) in its cell sap against a concentration gradient. Over a range of Mg(2+) in the growth medium from 5 x 10(-5) to 1.35 x 10(-2)m, the concentration of Mg(2+) in the cell sap of B. cereus T was maintained at about 6 x 10(-3)m, and ribosome-bound Mg(2+) and spermidine, as well as the spermidine concentration in the cell sap, appear to be unaffected by the concentration of Mg(2+) in the growth medium. Inhibition of growth of E. coli by streptomycin is progressively reversed by increasing the concentration of Mg(2+) in the growth medium above 5 mm. The finding that similar increases of Mg(2+) in the growth medium did not reverse the inhibition of B. cereus T is also consistent with the conclusion that B. cereus T, unlike E. coli, accumulates Mg(2+) to a constant concentration in its cell sap.


Purification and properties of glycogen isolated from a blue-green alga, Nostoc muscorum. Alpha granules were isolated from a blue-green alga, Nostoc muscorum, in large quantities and high purity by sucrose density gradient centrifugation. Sodium deoxycholate was used to eliminate membrane contamination. Isolated alpha granules from this species have average dimension of 31 nm in width and 65 nm in length. Each alpha granule consists of two equal parts. Attempts to dissociate the intact granule under mild conditions into relatively large subunits did not succeed. Analytical centrifugation of the alpha granules yields a sedimentation coefficient of 265S (S(20, w)). Chemical analysis reveals that alpha granules contain highly branched polyglucosyl units with short external chain lengths.


Sporulation of a cortexless mutant of a variant of Bacillus cereus. A stage 4 sporulation mutant of a strain of Bacillus cereus var. alesti fails to synthesize a cortex although all other structural components appear normal. With terminal lysis the spore core as well as the sporangium is lysed. Both the uptake of (45)Ca and the synthesis of dipicolinic acid (DPA) are similar to these activities in the parent strain, but these components (DPA and Ca) are lost to the medium with the drastic lysis. The first stage of diaminopimelic acid incorporation, that into germ cell wall mucopeptide, is intact in the mutant; the second stage, that into cortical mucopeptide, is absent. These biochemical studies as well as phospholipid metabolism and freeze-etch analysis suggest the lesion lies in the outer forespore membrane.


Ultrastructural changes associated with activation and germination of Bacillus cereus T spores. The ultrastructural changes occurring during defined stages of the transition of dormant Bacillus cereus T spores into heat-sensitive forms were investigated. The coat of the heat-activated spores displayed conspicuous striations across its middle layer. Electron microscopy of thin sections of heat-activated spores revealed the presence in the spore of a layer consisting of hexagonally oriented subunits. It was demonstrated that the subcoat region, but not the cortex, disappears rapidly during germination of B. cereus T spores. The fibrous structures apparently associated with the spore coat remain virtually unchanged during the entire course of activation and germination.


Purification and thermal stability of intact Bacillus subtilis flagella. Flagella were prepared and purified in a relatively intact form from bacterial lysates. Immunochemical tests showed that over 95% of the protein in the final preparation consisted of flagellar antigen. These flagella are more stable to thermal denaturation than flagella filaments obtained by shearing. Their thermal properties more closely resemble those of flagella in the native state on bacteria. The presence of the hook structure is responsible for this extra stability.


Purification of intact flagella from Escherichia coli and Bacillus subtilis. A procedure is described for the purification of bacterial flagella in the form of a filament-hook-basal body complex (intact flagella) free from detectable cell wall, membrane, or cytoplasmic material. Spheroplasts produced with lysozyme and ethylenediaminetetraacetic acid were lysed with Triton X-100, and the flagella were purified by (NH(4))(2)SO(4) precipitation, differential centrifugation, and CsCl gradient centrifugation. As much as 40% of the flagella were recovered, and they contained about one basal body per 4 to 6 mum of flagella. The same procedure developed for Escherichia coli was also successful for purifying intact flagella from Bacillus subtilis.


Fine structure and isolation of the hook-basal body complex of flagella from Escherichia coli and Bacillus subtilis. The hook-basal body complex comprising the basal end of purified intact flagella from Escherichia coli and Bacillus subtilis was studied in detail with an electron microscope. The E. coli hook can be described as having five or six concentric helical coils. The basal body from E. coli is 27 nm in length and consists of four rings, 22.5 nm in diameter, arranged in two pairs and mounted on a rod. The top pair of rings is connected near their periphery, resembling a closed cylinder. In B. subtilis the basal body looks like that from E. coli, except that the top pair of rings is missing. Hook-basal body complexes from both organisms could be isolated by dissociating the filaments with either urea or acid. Based on our results, two types of basal body structures are proposed, as exemplified by E. coli and B. subtilis, which directly reflect the structure of the gram-negative and gram-positive cell envelopes.


Control of tryptophan biosynthesis by the methyltryptophan resistance gene in Bacillus subtilis. 5-Methyltryptophan-resistant mutants derived from Bacillus subtilis strain 168 synthesize all of the tryptophan biosynthetic enzymes constitutively and excrete tryptophan. These mutants can be divided into three classes: class 1, low enzyme level and low rate of tryptophan excretion; class 2, high enzyme level and intermediate rate of tryptophan excretion; and class 3, high enzyme level and high rate of tryptophan excretion. A bradytrophic requirement for phenylalanine is correlated with the rate of tryptophan excretion. The phenylalanine requirement is relieved when the rate of tryptophan excretion is reduced by either (i) lowering the level of the tryptophan enzymes, (ii) reducing the supply of a tryptophan precursor (chorismate), or (iii) stopping tryptophan synthesis by a mutational block in the pathway. All of the mutants map in a region of the chromosome previously reported as the mtr locus. Our data show that synthesis of the tryptophan enzymes is controlled through the mtr locus but not influenced by precursors of tryptophan.


Biochemical and genetic characterization of a temperature-sensitive, tryptophanyl-transfer ribonucleic acid synthetase mutant of Bacillus subtilis. A temperature-sensitive, 5-fluorotryptophan (5FT)-resistant mutant of Bacillus subtilis was isolated which forms an altered tryptophanyl transfer ribonucleic acid synthetase [l-tryptophan: sRNA ligase (AMP), EC 6.1.1.2]. The mutant grows well at 30 C but not at 42 C. At the latter temperature, protein and ribonucleic acid (RNA) synthesis are abolished while deoxyribonucleic acid (DNA) synthesis proceeds for a considerable time. Tryptophanyl-transfer RNA (tRNA) synthetase activity is not detectable in the extracts of the mutant grown at 30 C whether this activity is measured by the attachment of l-tryptophan to tRNA or the l-tryptophan-dependent exchange of (32)P-pyrophosphate with adenosine triphosphate. Mixing experiments with extracts from the wild type and the mutant have ruled out the presence of an inhibitor or the absence of an activator as possible causes. Attempts to retrieve enzyme activity in vitro by various means (different conditions for cell disruption, addition of l-tryptophan, and adenosine triphosphate to the extraction buffer containing glycerol) were unsuccessful. The mutation in the locus of the tryptophanyl tRNA synthetase (trpS) was mapped on the bacterial chromosome by transformation and transduction. It is located between argC and metA. All temperature-resistant transformants recover wild-type levels of tryptophanyl tRNA synthetase activity and sensitivity to 5FT. Spontaneous revertants to temperature resistance are 5FT sensitive, but their levels of tryptophanyl tRNA synthetase activity and the thermolability of this enzyme in cell-free extracts varies. These revertants do not support the growth of a presumed nonsense mutant of phase SPO-1. Transduction experiments with phage PBS-1 indicated that reversion must be the result of an event at the site of the original mutation or at a site extremely close to it.


Lesions of the feet in athletes.

Lesions of the feet in athletes.


Duodenal diverticulosis and chronic diarrhoea.


Park-Williams number 8 strain of Corynebacterium diphtheriae. Five clones of the Park-Williams number 8 strain of Corynebacterium diphtheriae, previously maintained in separate laboratories, were examined for their colonial and biochemical properties, for the restriction and modification system which operates to obscure their lysogeny, and for their capacity to produce large amounts of toxin under ordinary laboratory conditions. The phenotypes of their phage, P, produced in strain 603 and C7 (P.603 and P.C7) differ both as to stability to storage in the cold and to inactivation by antiphage serum. Evidence for a high degree of stability in the integration of P prophage in the PW8 genome is presented.


Nucleic acid synthesis in Bacillus subtilis infected with bacteriophage beta-22. Changes in the synthesis of host and phage nucleic acid after infection of Bacillus subtilis with virulent bacteriophage beta22 were analyzed by deoxyribonucleic acid (DNA)-DNA and ribonucleic acid (RNA)-DNA hybridization. Host DNA replication continued during the first third of the 55-min latent period and then ceased at approximately the time replication of the phage genome was initiated. Host-specific RNA was synthesized concurrently with phage RNA during the first half of the latent period but was repressed late in the infection. For much of the latent period, the population of phage-specific RNA changed continually as new species were transcribed and earlier species were repressed; detectable changes ceased coincidentally with the appearance of intracellular phage. Control over transcription of phage DNA was to some degree an intrinsic property of the interaction of B. subtilis DNA-dependent RNA polymerase and the phage genome, since only the early species of phage RNA were synthesized in vitro by B. subtilis polymerase and pure beta22 DNA. In vitro transcription of late functions was demonstrated by using the endogenous RNA polymerase activity of the nucleoprotein complex (nuclear fraction) from infected cells.


Development of bacteriophage alpha: requirement for the integrity of one strand of parental deoxyribonucleic acid. Bacteriophage alpha-Bacillus megaterium complexes are as sensitive as free phage to the decay of (32)P incorporated in parental phage deoxyribonucleic acid during the eclipse period.


The interaction of fungal laccase with hydrogen peroxide and the removal of fluoride from the inhibited enzyme.


Reduction of activity of reduced nicotinamide adenine dinucleotide oxidase by divalent cations in cell-free extracts of Bacillus cereus T. A rapid and effective method was devised for the reduction of activity of reduced nicotinamide adenine dinucleotide (NADH) oxidase in crude extracts of Bacillus cereus T. The addition of 25 mumoles of MnCl(2) per mg of extract protein in tris(hydroxymethyl)aminomethane-hydrochloride buffer reduced NADH oxidase activity by 90% within 1 min, and this reduction was independent of pH between pH 7.0 and 8.5. Other divalent cations such as Mg(2+), Ba(2+), Ca(2+), and Co(2+) also reduced NADH oxidase activity, but monovalent cations such as Na(+) and K(+) were ineffective. The reduction of NADH oxidase activity by divalent cations was presumably due to the removal of an essential flavine cofactor, since the addition of riboflavine and flavine mononucleotide to treated extracts was shown to completely restore NADH oxidase activity. The specificity, convenience, and efficiency of the procedure were shown to be applicable to crude extracts of B. megaterium and B. subtilis and should facilitate spectrophotometric measurements of nicotinamide adenine dinucleotide-dependent dehydrogenases in these and other microorganisms.


Mode of action of streptozotocin.

Mode of action of streptozotocin. Streptozotocin induces rapid degradation of deoxyribonucleic acid (DNA) in actively dividing or resting Bacillus subtilis cells. Difference spectroscopy showed that the antibiotic interacts specifically with cytosine containing mononucleotides in vitro. This interference occurs only within the very narrow pH range of 5 to 5.5 and is reversed immediately upon lowering or increasing the pH. No measurable interaction was observed between isolated DNA and streptozotocin. The possibility is discussed that interaction of streptozotocin with cytosine residues in cellular DNA, although possibly taking place at a very low frequency, may constitute the primary step inducing DNA strand breakage.


Iron requirements and aluminum sensitivity of an hydroxamic acid-requiring strain of Bacillus megaterium. Bacillus megaterium strain ATCC 19213 secretes a ferric-chelating secondary hydroxamic acid, whereas a mutant (strain SK11) derived from it cannot produce a hydroxamate. Strain SK11 could be cultivated in a sucrose-mineral salts medium (treated with Chelex 100 to reduce trace metals) in the absence of added hydroxamate, if the inoculum was high. The lowest iron supplements necessary for maximal growth of both strains were equivalent (0.01 to 0.04 mug of iron per ml). Addition of either aluminum (0.5 mug/ml) or chromium (0.1 mug/ml) to the medium prevented full growth of strain SK11 at the minimal iron concentration, although elevated iron (1 mug/ml) reversed this inhibition. The iron-free secondary hydroxamate, Desferal, also abolished aluminum and chromium inhibition of strain SK11, producing maximal population densities at the low iron concentration. Growth of the hydroxamate-producing strain 19213 was not altered significantly by the aluminum or chromium levels which inhibited strain SK11. However, strain 19213 responded to these metals by increasing its secretion of a secondary hydroxamate. It was concluded that aluminum and chromium interfered with iron incorporation, either directly or by formation of nonutilizable aggregates with iron. The secondary hydroxamates may have overcome this interference by solubilization of iron for delivery to a single uptake process, or the ferric-hydroxamate chelate may enter the cell by an alternate route.


Regulation of chromosome replication in Bacillus subtilis: effects of amino acid starvation in strain W23. Amino acid starvation allows limited synthesis of deoxyribonucleic acid (DNA) in Bacillus subtilis strain W23. DNA synthesis increased by about 30% after leucine starvation and by about 60% after histidine starvation. Genetic analysis on the DNA synthesized after amino acid starvation showed that all genetic markers examined have replicated, regardless of which amino acid was starved for. Initially, all markers replicated equally, but upon further replication, the thr cysB and the argA to lys regions replicated ahead of their neighboring, proximal regions. This could indicate that preferred stopping sites exist in these regions or additional sites from which replication can originate reside there. The results suggest that chromosome replication continues from those sites where it had stopped during amino acid starvation.


Mutant of Bacillus subtilis demonstrating the requirement of lysis for growth. A temperature-sensitive mutant of Bacillus subtilis which has impaired ability to autolyze was isolated. The growth rate of mutant cells at high temperature can be increased by the addition of egg-white lysozyme or a B. subtilis autolysin. Experiments with this mutant show that lysis is essential for cell growth.


Chl b redundant?

Chl b redundant?


Peptidoglycan differences in strains of Bacillus cereus constitutive and inducible for penicillinase production. Strains of Bacillus cereus differing in penicillinase production were shown to possess cell walls of differing muramic acid contents.


The arterial supply to the pelvic limb of the domestic cat.


Neurohistological changes in the central nervous system of the bull "Bosfor" affected with paresis spastica.


Effect of chloral hydrate and related compounds on the fermentation of glucose by rumen bacteria.


Effect of chloral hydrate and related compounds on the metabolism of lactic and pyruvic acid by rumen bacteria.


Investigations on urinary estrogen excretion from ewes with special reference to the clinical condition of the genital tract. II. Pregnancy and puerperium.


Influence of climate on thyroidal iodine metabolism in buffalo-cows. I. Thyroidal iodine concentration.


Nuclear magnetic relaxation dispersion in protein solutions. IV. Proton relaxation at the active site of carbonic anhydrase.


Phenylketonuria.

Phenylketonuria.


Valerianie, a tertiary monoterpene alkaloid from valerian (1).


Fourier transform 13C-NMR spectroscopy of biologically active cysteine peptides.


Gas chromatographic identification of the thiohydantoins of degradation products of peptides and proteins.


In vitro susceptibility of Brucella to various antibiotics. A series of 27 strains of six species of Brucella was tested for susceptibility in vitro to a representative cross section of antibiotics in current use. The activity against each species was plotted, with the cumulative per cent of strains inhibited indicated for each concentration. As a class, the tetracycline antibiotics were the most effective. Erythromycin, gentamicin, streptomycin, and kanamycin, as well as rifampin, were quite active. The penicillin-cephalosporin group, with the exception of ampicillin, was comparatively ineffective, as were the polypeptides and the miscellaneous group of chloramphenicol, lincomycin, cycloserine, and sulfadiazine. Species differences were noticeable, with some strains of B. canis being considerably more resistant to streptomycin and the tetracyclines than B. suis and B. abortus. B. melitensis, B. ovis, and B. neotomae were intermediate in antibiotic susceptibility.


Effect of relative humidity on the penetrability and sporicidal activity of formaldehyde. The effect of relative humidity (RH) on formaldehyde penetration of paper, glassine, and cotton was determined by the death rate of bacterial spores in glass tubes covered with these materials. The data show that paper is readily penetrated regardless of RH, but the RH greatly affects the penetration rate of glassine and cotton. A comparison was also made of the effect of RH on the penetrability of formaldehyde generated from Formalin and paraformaldehyde. At low RH, all three closures were penetrated more readily by formaldehyde from paraformaldehyde than from Formalin, but no difference in the two was observed at high RH. It is felt that the difference at low RH is primarily due to the condensation of the vaporized Formalin.


Reversible inhibition of spore germination by alcohols. Low levels of alcohols have been found to inhibit the process of spore germination. The extent of germination is dependent upon the concentration of alcohol present in the germinating medium. This inhibition is reversible since removal of the alcohol from the spore environment allows germination to proceed.


Cobalt as a functional group.

Cobalt as a functional group.


Nucleic acid and protein synthesis, as measured by incorporation of tracers, during regeneration in ultraviolet-treated Blepharisma.


Melanin versus protein synthesis in melanocytes in vitro.


Training in national health planning. Report of a WHO expert committee.


Temperature-mediated hyper-induction of fatty acid desaturating enzyme.


Bacillus subtilis glutamine synthetase. Specific catalytic changes associated with limited sulfhydryl modification.


Electron spin resonance of chlorophyll and the origin of signal I in photosynthesis. A comparison has been made between Signal I, the photo-electron spin resonance signal associated with the primary light conversion act in photosynthesis, and free-radical signals generated in various chlorophyll species in vitro. The esr signals obtained from chlorophyll.monomer, (Chl.L)(+.), chlorophyll dimer, (Chl(2))(+.), and chlorophyll oligomer, (Chl(2))(n) (+.), are broader than Signal I, whereas the chlorophyll-water adduct, (Chl.H(2)O)(n) (+.), gives a signal very much narrower than Signal I. The unusually narrow signal from (Chl.H(2)O)(n) (+.) has been ascribed to spin migration, or to unpaired spin delocalization over a large number of chlorophyll molecules. The linewidth of Signal I can be accounted for by a similar delocalization process. A theoretical relationship between the esr linewidth and the number of chlorophyll molecules, N, over which an unpaired spin is delocalized, takes the form DeltaH(N) = 1/ radicalN.DeltaH(M), where DeltaH(M) is the linewidth of monomer (Chl.L)(+.). This relationship for N = 2 accounts well for the linewidths of Signal I in green algae, blue-green algae, and photosynthetic bacteria in both the (1)H- and (2)H-forms. The linewidth of Signal I (as well as the optical properties of reaction-center chlorophyll) are consistent with unpaired spin delocalization over an entity containing two chlorophyll molecules, (Chl.H(2)O.Chl)(+.).


Nitrogen, phosphorus, and eutrophication in the coastal marine environment. The distribution of inorganic nitrogen and phosphorus and bioassay experiments both show that nitrogen is the critical limiting factor to algal growth and eutrophication in coastal marine waters. About twice the amount of phosphate as can be used by the algae is normally present. This surplus results from the low nitrogen to phosphorus ratio in terrigenous contributions, including human waste, and from the fact that phosphorus regenerates more quickly than ammonia from decomposing organic matter. Removal of phosphate from detergents is therefore not likely to slow the eutrophication of coastal marine waters, and its replacement with nitrogen-containing nitrilotriacetic acid may worsen the situation.


Sixty-six year old caucasian female with progressive somnolence.


Treponematoses research. Report of a WHO scientific group.


The effect of some factors on the cell volume of rumen ciliates.


Synthesis of glutamate by a glutamine: 2-oxo-glutarate amidotransferase (NADP oxidoreductase) in Bacillus megaterium.


The similarity of tryptophan synthetases of Anabaena variabilis and Chlorella ellipsoidea with that of bacteria.


Effects of phosphate, HEPES, N2O and CO on the kinetics of human erythrocyte carbonic anhydrases B and C.


Diverticula of the female urethra: assay of 120 cases.


Small intestinal bacterial flora and folate status in gastrointestinal disease. Serumfolate folate levels and the bacteriology of the small intestine were studied in 13 patients with jejunal diverticulosis, 29 patients with partial gastrectomy, and five patients with ileal disease. The mean serum folate level in the patients with partial gastrectomy (7.2 mmug/ml) was similar to that of control subjects but the mean level in the patients with jejunal diverticulosis (14.6 mmug/ml) was significantly higher than the mean level of the control group (8.0 mmug/ml). Five of the 13 patients with jejunal diverticulosis and two of the patients with partial gastrectomy had levels above the upper limit of the control group (> 16.6 mmug/ml), and five of six patients with jejunal diverticulosis studied excreted raised amounts of folate in the urine (> 13.2 mug in 24 hours). Serum folate in one of these patients with jejunal diverticulosis was identified chromatographically as 5-methyltetrahydrofolic acid. The mean serum folate level (9.0 mmug/ml) in the patients with partial gastrectomy who had Esch. coli in the jejunal aspirates was significantly higher than in those without Esch. coli present (mean 4.2 mmug/ml). It is suggested that in gastrointestinal disease jejunal bacteria may contribute to the serum folate level even though this remains in an accepted normal range. Some patients had low serum folate levels despite having a large number of Esch. coli in the jejunum. No evidence of malabsorption of folic acid was found in three such patients even though one had lactobacilli capable of consuming folate in the jejunum. No relationship was found between serum folate level and ileal bacteriology.


Inhibition of serine proteases by arylboronic acids. Arylboronic acids were found to be strong competitive inhibitors of subtilisin and chymotrypsin. The binding constants are strongly pH dependent and give a Hammett-type plot with a slope of -0.885. The pH dependence, the Hammett plot, and nmr model-system studies indicate that inhibition is due to electron-pair donation by the active site histidine to the bound inhibitor.


Histochemical study of polysaccharides in the arch and thoracic aorta of Gallus gallus (Linnaeus 1758) during the development.


Ultrahistochemical study of the carbohydrate cell coat (glycocalyx) in the lung of the rat.


Structural changes of cortex adrenalis after the administration of 2-thio-6-azathymine.


A histochemical and karyometric study on the effect of stilboestrol and testosterone on adrenal cortex of adult male rat.


Studies on the mouse mammary tumour agent (MTA). II. Cytochemistry of aminopeptidase in normal mammary gland of C57BL, spontaneous and virus-induced mammary tumours in R3 and C57BL mouse strains, respectively, and in cell lines producing the mammary tumour virus.


Enzyme distribution patterns in the granules of eosinophil leukocytes of the mouse.


Histochemical studies on calcification of the rat testis by lead acetate.


Cytochemical studies on the origin of bizarre, acid phosphatase-rich structures in rat liver cells.


Histochemical demonstration of sulphated polysaccharides at the surface coat of nerve cells in the mouse central nervous system.


Histochemical examinations at vital and postmortem injuries.


Histochemical behaviour of the acetylcholinesterase in the myometrium of the albino rats, during estrus, diestrus, pregnancy and parturition.


Fluorogenic amines in the C-cells thyroid tumours of man.


Beta-glucuronidase in kidney-adrenal of male frogs (Rana tigrina): a correlation of observations on histochemistry and biochemistry.


Effects of an antiandrogen or castration on the plasmalogen concentration in the kidney of the male rat and on the hematocrit.


Reaction on histones with ammoniacal silver in the developing rat retina.


Demineralised dentin as substrate for Bacillus subtilis.


Comparative study of diabetes knowledge among juvenile diabetics and their parents.


Diglucosyldiglyceride from B. cereus.

Diglucosyldiglyceride from B. cereus.


Human carbonic anhydrases. VI. Levels of isozymes in old and young erythrocytes and in various tissues.


Inducible transport of citrate in a Gram-positive bacterium, Bacillus subtilis.


Urinary cholesterol. IV. Its excretion in women with neoplasms of the genital system.


Ontogeny and hormonal control of polyphenoloxidase isozymes in tobacco pith. Isozymes of tobacco pith polyphenoloxidases (o-diphenol oxidase, EC 1.10.3.1) were separated electrophoretically from fresh pith of intact plants and from cultured pith sections. Extracts of fresh pith contained a poorly resolved complex of two to three anodic bands after starch gel electrophoresis at alkaline pH. This anodic complex was more active with chlorogenic acid than with 3,4-dihydroxyphenylalanine and was found in greater activity per gram fresh weight of tissue in younger internodes than in older ones. The longitudinal gradient of activity was thus the opposite of that found for the constitutive isozymes of peroxidase.A well defined cathodic band of polyphenoloxidase activity appeared after culture of pith in modified White's medium with shaking. This band, which was more active with 3,4-dihydroxyphenylalanine than with chlorogenic acid, could be detected after 1 to 2 days of incubation. Its appearance was enhanced by the addition of 10 mum indoleacetic acid; kinetin (1 mum tended to prevent this indoleacetic acid effect). Such hormonal control is opposite to that previously reported for the rapidly appearing new isozymes of peroxidase. The pattern of the major isozymes associated with polyphenoloxidase activities differs from that of peroxidase.


Some aspects of the localization of phycobiliproteins in the Rhodophyceae and the Cyanophyceae.


Binding of D-glucose to the cyst coat of Colpoda steinii and metabolism of the sugar during excystment.


Biosynthesis of the cyst-coat protein of Colpoda steinii.


Fluorescence technique for studying the binding of sulphonamide and other inhibitors to carbonic anhydrase.


Fate of transforming DNA in Bacillus subtilis strain sensitive to methyl methanesulfonate.


Isolation of glucosaminyl-beta(1-4)-muramic acid and phosphoric acid ester of this disaccharide from acid hydrolysates of peptidoglycan of Bacillus cereus AHU 1356 cell walls.


Occurrence of non-N-substituted glucosamine residues in lysozyme-resistant peptidoglycan from Bacillus cereus cell walls.


Multipurpose serological surveys and WHO serum reference banks. Report of a WHO Scientific Group.


Mitomycin derivatives. 2. Derivatives of decarbamoylmitosane and decarbamoylmitosene.


Acid phosphatase release from intact phagocytic cells surrounding a large-sized parasite.


Jejunal diverticula.

Jejunal diverticula.


Nuclear magnetic resonance studies of human carbonic anhydrase B. Histidine residues.


Studies on a carbonic anhydrase from the midgut epithelium of larvae of lepidoptera.


Nuclear magnetic resonance relaxation of water adsorbed on bacterial cell walls.


Attitudes and delay in cancer detection.


Distribution of calcium, carbonic anhydrase and alkaline phosphatase activities in the uterus and isthmus of young and old White Leghorn Hens.


Drosophila enzyme-genetics: a table.

Drosophila enzyme-genetics: a table.


A simple enzymatic method for the synthesis of 2-hydroxy[4-14C] estradiol.


The central lymphoid tissues of rabbits. II. Functional and morphologic studies in adult animals.


Optical and electron microscopical study of cytoplasmic granules in human carotid body, carotid body tumours and glomus jugulare tumours.


Epidural anaesthesia in surgery of the pancreas and biliary tract.


Volatile flavoring substances in foodstuffs.


The electron-accepting sites in Rhus vernicifera laccase as studied by anaerobic oxidation-reduction titrations.


Ludwig Heilmeyer.

Ludwig Heilmeyer.


Physical and catalytic properties of the purine nucleoside phosphorylases from cells and spores of Bacillus cereus T.


Gas vacuoles. Light shielding in blue-green algae.


The mesosome of Bacillus subtilis as affected by chemical and physical fixation.


On the possible function of coated vesicles in melanogenesis of the regenerating fowl feather. How tyrosinase becomes associated with the premelanosomes was investigated by histochemical demonstration of tyrosinase activity by the use of dihydroxyphenylalanine (DOPA) in melanocytes of regenerating fowl feathers. The reaction product of DOPA was localized in the anastomosing membrane tubules associated with the concave side of some dictyosomes of the Golgi apparatus and in coated vesicles most of which were in connection with the dictyosomes. No reaction product was found in early premelanosomes. In premelanosomes, the reaction product of DOPA appears first in vesicles approximately 400 A in diameter which are surrounded by a matrix with a characteristic periodicity. These observations seem to allow the speculation that the coated vesicles function in the transport of tyrosinase, and that the premelanosomes are formed in a process which is not necessarily dependent on the Golgi apparatus as was assumed earlier.


Phenol oxidase activity in the integument of the silkworm Bombyx mori infected with Beauveria bassiana and Spicaria fumoso-rosea.


Quantitative measurement of the inhibitory effect of penicillin G on Escherichia coli and Bacillus subtilis.


Effect of 5-bromouracil on the pattern of deoxyribonucleic acid replication in germinating Bacillus subtilis spores.


Congenital vesical diverticulum.

Congenital vesical diverticulum.


Microbiology of the external eye.

Microbiology of the external eye.


Physico-chemical aspects of ophthalmic and contact lens solutions.


Eye irritation from topically applied drugs and cosmetics: preclinical studies.


Gentamicin dosages for renal insufficiency. Adjustments based on endogenous creatinine clearance and serum creatinine concentration.


Pericarditis as a complication of meningococcal meningitis.


Purification of Clostridium botulinum type A toxin.


The chemical composition and electron microscopic appearance of a protein derived from urinary casts.


The management of small-bowel fistulas: an improved technique.


Time study of in vivo incorporation of 32P orthophosphate into phospholipids of chicken epiphyseal tissues.


An economic appraisal of a preventative medicine program for dairy herd health management.


A reevaluation of nuclear and nucleolar changes induced in vitro by actinomycin D.


Isolation of enzymatically active nuclei from epithelial cells of the trachea.


The separation of different cell classes from lymphoid organs. IV. The separation of lymphocytes from phagocytes on glass bead columns, and its effect on subpopulations of lymphocytes and antibody-forming cells. Four separate effects can be demonstrated when lymphoid cell suspensions are passed through columns of siliconed glass beads. (a) A temperature-dependent "active adherence" of phagocytic cells, such as macrophages and polymorphs. (b) A temperature-independent and selective trapping by "physical adherence" of particular classes of lymphoid cells, including certain antibody-forming cells. (c) A "size-filtration" effect that traps larger cells, but only becomes significant with beads below 100 micro in diameter. (d) A selective retention of damaged cells, which occurs with all columns under all conditions tested. An active adherence column technique has been developed to separate phagocytes from lymphocytes while minimizing selection within the lymphocyte population by physical adherence or size filtration. In less than 10 min at 37 degrees C it reproducibly produces a preparation of mouse spleen lymphocytes >500-fold depleted of active macrophages, and approximately 50-fold depleted of active polymorphs, with good over-all cell recoveries and cell viability. The lymphocyte fraction appears fully active in its ability to initiate immune responses to at least two different antigens, but is changed in over-all composition and selectively depleted in certain classes of antibody-forming cells.


The morphology of hormone induced structural changes in the female breast.


Acid gel reaction of serum and pyroglobulins in 5 cases of multiple myelomas and a case of macroglobulinemia.


Osmosensitive single neurones in the hypothalamus of unanaesthetized monkeys. 1. We recorded with tungsten micro-electrodes the activity of single neurones in the supraoptic nucleus (NSO) and adjacent regions of the hypothalamus while repeatedly injecting solutions of varying tonicity into the common carotid artery of trained, unanaesthetized monkeys who accepted the experimental restraints without anxiety.2. Intracarotid injections of mildly hypertonic solutions of sodium chloride produced a characteristic behavioural response during and immediately after injection: e.e.g. ;arousal,' lip and tongue smacking, chewing, irregular sniffing respiration and associated mildly increased movement of face, eyes and body.3. Of the 130 cells analysed during hypertonic intracarotid injections, 105 (81%) were osmosensitive. Twenty-five (19%) of the cells studied during similar injections were non-osmosensitive. On the basis of the anatomical location of the cells, the pattern of discharge to intracarotid osmotic stimuli and the response to arousing sensory stimuli, we divided the osmosensitive cells into two major groups, ;specific' and ;non-specific' osmosensitive cells.4. Fifty-two (50%) of the osmosensitive cells we labelled ;specific' because they responded to an intracarotid injection of hypertonic sodium chloride, generally did not respond to non-noxious arousing sensory stimuli and were located in or near the supraoptic nucleus. We found two subtypes of these ;specific' osmosensitive cells: (a) twenty-one (20%) NSO cells with ;biphasic' responses, that is, acceleration followed by inhibition; (b) thirty-one (30%) cells in the immediate perinuclear zone of the NSO with ;monophasic' responses, subdivided into twenty-one (20%) cells that accelerated and ten (10%) that were inhibited.5. Fifty-three osmosensitive cells (50%), located diffusely in the anterolateral hypothalamus, were ;non-specific', responding both to intracarotid injections of hypertonic sodium chloride and also to sensory stimuli that were mildly arousing. Two groups of ;non-specific' osmosensitive cells showed monophasic responses; thirty-five (34%) cells accelerated and seventeen (16%) of them were inhibited.6. The ;monophasic' specific osmosensitive neurones lying in the immediate perinuclear zone of the supraoptic nucleus in the primate could conceivably be the ;osmoreceptors' of Verney. The ;biphasic' specific osmosensitive neurones in the NSO may well represent the secretory cells of this system. From our data, the ;non-specific' osmosensitive neurones, scattered diffusely in the anterolateral hypothalamus, have little to do with osmoregulation. Some of these cells located in the perinuclear zone of the NSO could act as interneurones, however, conveying afferent input to the osmoreceptor-secretory complex of the supraoptic nucleus.


Clinical applications of the Australia antigen test.


Elevated urinary secretory IgA in children with urinary tract infection.


The prevention of perinatal mortality and morbidity. Report of a WHO Expert Committee.


Pathogenetic and serologic studies of type 1 and type 2 plaque-producing agents derived rom Cal-1 strain of Marek's disease virus.


Infection with chloroquine-resistant Plasmodium falciparum in man: prepatent periods, incubation periods, and relationships between parasitemia and the onset of fever in nonimmune persons.


Anthrax.

Anthrax.


Current status of lactose-fermenting Neisseria.


Listeria infections.

Listeria infections.


Similarities of mycoplasma species isolated from man and from nonhuman primates.


Serologic studies of staphylococcal and streptococcal L-forms.


Marburg virus.

Marburg virus.


Studies of Epstein-Barr virus.

Studies of Epstein-Barr virus.


A "virus" associated with serum hepatitis.


Studies on new virus isolates recovered in tracheal organ culture.


Purification and characterization of penicillinases from Salmonella typhimurium and Escherichia coli.


Purification and properties of carbonic anhydrase B from porcine erythrocytes.


Properties of a new form of DNA from whole calf thymus nuclei: evidence for reactive, special sites in DNA.


The alpha-amylases as glycosylases, with wider catalytic capacities than envisioned or explained by their representation as hydrolases.


Carbon dioxide tolerance after adaptation to hypercarbia.


L-iduronidase in cultured human fibroblasts and liver.


Adenosine triphosphate and inosine triphosphate dependent conformational changes of adenosine diphosphate-G-actin.


Affinity labeling of a mouse myeloma protein which binds nitrophenyl ligands. Sequence and position of a labeled tryptic peptide.


Influence of pH on the kinetics of complex formation between aromatic sulfonamides and human carbonic anhydrase.


A method for the collection of bovine seminal vesicle secretions for microbiologic examination.


Isolation of a virus from a female cat with urolithiasis.


Histochemical differentiation of two forms of glycogen synthetase.


Some factors affecting the viability of freeze-thawed T4 bacteriophage.


A new approach to development of live vaccine against tick-borne encephalitis.


Protection from microbial contamination in a room ventilated by a uni-directional air flow.


Gargoylism in a Chinese boy.

Gargoylism in a Chinese boy.


Bacterial negativity and reactivation (relapse) of lepromatous outpatients under sulfone treatment.


Hypergammaglobulinemia, secondary macroglobulinemia and paraproteinemia in leprosy.


Persistence of Brucella abortus, strain 19 infection in immunized cattle.


Bovine ephemeral fever. II. Responses of cattle to attenuated and virulent virus.


Serologic survey of toxoplasmosis in Southern California domestic animals by a hemagglutination, microtiter method.


Inhibition of phospholipase A-induced swelling of mitochondria in the energized state.


Changes in high-density lipoprotein protein composition after heparin-induced lipolysis.


Dissociation by lanthanum of smooth muscle responses to potassium and acetylcholine.


Some aspects of plaque formation by human influenza viruses. I. Cell and media factors affecting a modified plaque technique.


Betonite flocculation test for the detection of foot-and-mouth disease antibodies.


Some aspects of plaque formation by human influenza viruses. II. Viral factors influencing plaque production.


Characteristics of gel precipitating antigens of rubella virus.


Non-genetic reactivation of differently inactivated vaccinia virus.


Effect of physical and chemical treatment on the buoyant density of reovirus.


Respiratory syncytial virus tissue culture immunofluorescence as a laboratory aid.


Neutralization of equine arteritis virus: enhancing effect of guinea pig serum.


Characteristics of the BGM line of cells from African green monkey kidney. Brief report.


Parana, a new Tacaribe complex virus from Paraguay.


Tests for the hepatitis-associated antigen. Brief report.


Some quantitative observations on the cochlear division of the eighth nerve in the squirrel monkey (Saimiri sciureus).


Intracarotid air embolism in the baboon: effects on cerebral blood flow and the electroencephalogram.


Immunological relatedness of nerve growth factors.


The clinical symptomatology associated with the 6 c-sec spike and wave complex.


Hyperlactatemia.

Hyperlactatemia.


Electron microscopic studies on the pupillary membrane: the fine structure of the white strands of the disappearing stage of this membrane.


Lactic acidosis through medicaments.

Lactic acidosis through medicaments.


Giant jejunal diverticulosis: a rare cause of massive upper gastro-intestinal hemorrhage.


Is the bubble bursting?

Is the bubble bursting?


RNA dependent DNA polymerase activity in mammalian cells.


Effect of Apollo 11 lunar samples on terrestrial microorganisms.


Some questions on nephrotoxicity.

Some questions on nephrotoxicity.


Physiologic significance of contrast-protein interactions. I. Study in vitro of some enzyme effects.


Spontaneous and induced abortion. Report of a WHO scientific group.


Histochemical study of the metabolism of the ketone bodies in the nervous system. IV. Localization of the D(-)-beta-hydroxybutyric dehydrogenase in cerebellum and medulla oblongata.


Dual cytologic localization of beta-glucuronidase in some organ-tissues of anuran tadpoles in metamorphosis.


Ultraimmunohistochemical demonstration of miyagawanella ornithosis antigen.


The reversible reaction of brome grass mosaic virus and its RNA with mercuric ions.


Proteins of cytoplasmic, chloroplast, and mitochondrial ribosomes of some plants.


What's wrong with health care in America?


Corynebacterium diphtheriae in skin lesions in Ugandan children. Diphtheritic skin lesions are known to be relatively common in tropical as opposed to temperate climates, but the possible presence of Corynebacterium diphtheriae in skin lesions has not been investigated in East Africa, where classical diphtheria cases are rare. The authors therefore studied septic skin lesions and skin ulcers in 139 preschool children and schoolchildren in Uganda. A high percentage (up to 60%) of C. diphtheriae lesions was found, a majority of the isolations being of the mitis type. At the same time, low respiratory carrier rates for C. diphtheriae were found. The practical importance of the skin reservoir of C. diphtheriae for the development of specific diphtheria immunity in the tropics is stressed.


A neural mechanism regulating follicular development in the hamster.


Neurosecretion in the median eminence of the axolotl after induction of metamorphosis.


Some factors affecting DNA-dependent RNA polymerase solubilization from rat liver nuclei.


Effect of Pseudomonas aeruginosa and Staphylococcus pyogenes on the rate of wound healing in mice.


The economics of health and disease.

The economics of health and disease.


Three serum enzymes in twins.

Three serum enzymes in twins.


Parasitic infestations of bovine heart in Uttar Pradesh.


In vivo and in vitro studies on the oxidation of ascorbic acid to dehydroascorbic acid in the testis of toad.


Studies on murine sarcoma virus. II. Detection of group-specific antigens by immunofluorescence.


Resistance to transplants of recent spontaneous parental line tumors by F1 hybrid hosts.


CIR (cell injuring reaction) active substances in culture fluid of Bacillus cereus "MS-1063 strain".


Experimental studies on the serum therapy of type E botulism: the relationship between the amount of toxin in the blood and the effect of antitoxic serum.


Swelling of mouse liver mitochondria induced by cord factor.


Structural and functional damage of mitochondria by diphtherial cord factor.


Antibodies to "alcalase" after industrial exposure.


Pollution through cleanliness?

Pollution through cleanliness?


Isoniazid treatment for tuberculosis.

Isoniazid treatment for tuberculosis.


Response of the subhuman primate in gram-negative septicemia induced by live Escherichia coli.


Comparison of DDT effect on pentobarbital metabolism in rats and quail.


The nutritive effect in man of non-essential amino acids infused intravenously (together with the essential ones). II. Different patterns of amino acids and different levels of nitrogen supply.


The nutritive effect in man of non-essential amino acids infused intravenously (together with the essential ones). 3. Different ratios of essential-non-essential amino acids.


On the relation between motor activity and the degree of enzyme inhibition following inhibition of tyrosine hydroxylase.


Scanning electron microscopy of zonulolysis by alpha chymotrypsin.


Experimental retinal detachment in the owl monkey. VII. Photoreceptor protein renewal in normal and detached retina.


Studies on muscle differentiation. II. Antigenicity of tropomyosin from frog skeletal muscles.


Changes in electronegativity of the cell surface during the development of the cell surface during the development of the cellular slime mold, Dictyostelium discoideum.


Studies in helminth enzymology. VI. Aminopeptidases from uterine extracts of Ascaris suum.


Ascaris suum: purification and characterization of an intestinal aminopeptidase.


Ascaris suum: immunoelectrophoretic analysis of antigens in developmental stages.


An efficient synthesis of thyrotropin releasing hormone (TRH).


Sero-chemical characterization of a soluble antigen extracted from Brucella abortus.


Carbonic anhydrase activity of pigeon erythrocytes.


Water soluble fluorescent pigment production by a strain of Pseudomonas aeruginosa.


An introspective view of penicillinase.

An introspective view of penicillinase.


In vitro susceptibility and resistance of gram negative bacilli to carbenicillin.


Ten years of international exchange of scientific publications by the Library of Bialystok Medical Academy.


A case of neuro-cutaneous melanosis.

A case of neuro-cutaneous melanosis.


Studies on cerebral lipidosis. Enzymatic diagnosis of metachromatic leukodystrophy.


Studies on the urinary amino acid patterns in the developing monozygotic and dizygotic twins. II. The individuality of the urinary amino acid patterns in the developing monozygotic and dizygotic twins.


Studies on the urinary amino acid patterns in the developing monozygotic and dizygotic twins. I. The effect of the diet on the urinary amino acid patterns of children.


Management of a case of crushed chest and multiple fractures who developed fat embolism on the thirteenth day.


Progressive muscular dystrophy in infancy.


1,4-benzodiazepines. Chemistry and some aspects of the structure-activity relationship.


A convenient synthesis of 2',3',5'-tri-O-acetyladenosine and -uridine.


Rhinocerebral phycomycosis.

Rhinocerebral phycomycosis.


Relative incorporation of phenylalanine at different temperatures by cell-free systems from Bacillus licheniformis and Bacillus stearothermophilus.


Repair of ultraviolet-damaged DNA in Micrococcus lysodeikticus. I. An endonuclease specific for ultraviolet-irradiated DNA.


Increase of thymidine, thymidylate and deoxycytidine kinase activites during germination of bacterial spores.


The metabolism of deoxyribonucleosides in Lactobacillus acidophilus: regulation of deoxyadenosine, deoxycytidine, deoxyguanosine and deoxythymidine kinase activ-ties by nucleotides.


A membrane-bound lysophospholipase from Mycoplasma laidlawii strain B.


Electrophoresis of colloidal biological particles.


A comparison of L-asparaginase from Erwinia aroideae and from Escherichia coli: biochemical and biological properties.


Quantitative studies on the accumulation of tetracycline in tumors.


Adhesion of blood platelets to subendothelial surface: distinct from adhesion to collagen.


On the mechanism of modification of radiation effect by dimethyl sulfoxide.


Changes observed in the FSH and LH cells of the adenoypophysis of Presbytis entellus entellus following cadmium induced testicular necrosis.


Munchmeyer's disease or fibrodysplasia ossificans progressiva.


Renal failure.

Renal failure.


Microbial suprainfection. Recognition and management.


Peptide chain initiation in homologous and heterologous systems from mitochondria and bacteria.


Immunochemical studies on structural proteins of the red cell membrane.


Thermolysin: kinetic study with oligopeptides.


The use of specimen roentgenography to detect small carcinomas not found by routine pathologic examination.


Radiographic identification of calcifications in breast specimens.


Clues to identifying colorectal cancers which do or do not tend to metastasize to lymph nodes.


Fishing for viruses in cancer cells.

Fishing for viruses in cancer cells.


When to hospitalize for metastatic carcinoma.


The effect of polynucleotides on phagocytosis of listeria by peritoneal macrophages.


Cancer statistics, 1971.

Cancer statistics, 1971.


Changes in the histaminolytic activity of the guinea pig liver and plasma during anaphylaxis and after heparin injection.


Bronchial eosinophilia in rhesus monkeys with IgE mediated respiratory responses.


Isolation of purified allergens (cod) by isoelectric focusing.


Electrical stimulation of inferotemporal and occipital cortex in monkeys: effects on visual discrimination and spatial reversal performance.


Atopic hypersensitivity to L-asparaginase. Resistance to immunosuppression.


Brainstem mechanisms underlying visually guided responses in the rhesus monkey.


Studies on the thyroid gland component inhibiting LATS.


Immunodiffusion and immunoelectrophoretic techniques.


A study of the incidence of Australia antigen and antibody in nonhuman primates.


Application of the microtiter complement fixation technique to studies of hepatitis associated antigen in human hepatitis.


Precipitating heterologous antisera for the detection of Au-SH-antigen.


Screening for hepatitis associated antigen by three different methods.


The Australia antigen in chronic active hepatitis.


Technical aspects and results of the determination of hepatitis associated antigen (HAA) and antibody.


Purification of Australia-SH-antigen by acid dissociation of Australia-SH-antigen-antibody complexes.


Erythrocytic isoantigens of rhesus monkeys: factors II-B and II-C.


Screening blood donors for Australia antigen carriers at the Broussais hospital.


Isolation and characterization of two virulent antigens of Streptococcus dysgalactiae.


Hyperimmunization of rabbits with Australia antigen.


Identification of influenza A viruses by immunodiffusion test.


An allergic-type response.

An allergic-type response.


The Australia antigen and blood transfusion.


Evaluation of frequency of Australia antigen in blood donors of Tokyo by means of immune adherence hemagglutination technique.


Experiences on Australia antigen in Italy.


The incidence of hepatitis-associated antigen and antibody in patients with thalassaemia in Greece.


Australia antigen in various plasma fractions.


Study of the Australia antigen and of the corresponding antibody in blood donors in Paris.


Australia antigen in hemodialysis and renal transplantation units.


A Caucasian population with a high frequency of Au 'carriers': genetic analysis of the condition.


Epileptic seizures and the stereotaxic amygdalotomy.


Paramedial cerebellar nuclear lesions.

Paramedial cerebellar nuclear lesions.


Forel-H-tomy for the treatment of epilepsy.


Stereotaxic thalamotomy (lamella medialis) in aggressive psychiatric patients.


The effect of DL dopa on harmaline induced tremor and on resting tremor in monkeys with tegmental lesions.


Comparative biochemistry of the primates. I. Amino acid patterns in the blood and urine.


Comparative biochemistry of the primates. II. The indole compounds of primate blood and urine.


Reproduction in the lesser bushbaby (Galago senegalensis moholi) under semi-natural conditions.


Behaviour and female reproductive cycles in a captive group of Mangabeys.


The social organization of a semifree-ranging troop of squirrel monkeys (Saimiri sciureus).


Water-contact behavior of chimpanzees.

Water-contact behavior of chimpanzees.


The histology of the primate urethra.

The histology of the primate urethra.


Intrarenal arterial patterns in nonhuman primates.


Placenta previa in a patas monkey (Erythrocebus patas).


Flexion and metric age changes of the cranial base in the Macaca mulatta. I. Infant and juveniles.


The hemogram of the colonized lesser bushbaby (Galago senegalensis).


The phlogistic role of C3 leukotactic fragments in myocardial infarcts of rats. An improved method is described for extraction from rat myocardium of an enzyme that cleaves the third component of complement (C3) into leukotactic fragments. Direct cleavage of C3 has been shown by the use of a radiolabeled preparation of human C3. The enzyme elutes slightly beyond (retarded to) an albumin marker in gel filtration. After surgical ligation of a coronary artery, leukotactic activity can be extracted from infarcted rat myocardium. Described in terms of its time of appearance in infarcted tissue, the leukotactic activity has been identified as cleavage products of rat C3. The C3 fragments are of low molecular weight and heterogenous in size. Rats, if treated with the C3 inactivator (isolated from cobra venom) so as to ablate serum C3, fail to develop leukotactic activity in the infarcted myocardium. In turn, few if any of the usual intense accumulations of neutrophils in response to infected myocardium are seen during the first 48 hr after coronary artery ligation. These studies suggest a nonimmunologic role for C3 in the mediation of the acute inflammatory response in nonspecific tissue injury.


Total intravenous nutrition in newborn surgical patients: a three-year perspective.


Infantile pyloric stenosis associated with major anomalies of the alimentary tract.


Isospora papionis n. sp. (Eimeriidae) of the chacma baboon Papio ursinus (Kerr, 1792).


The fine structure of the sporozoite of Lankesteria culicis.


Behavioral effects of pargyline in primates.


Biosatellite post-flight experiment: some effects of forced electrolyte imbalance in Macaca nemestrina.


Intra- and interhemispheric projections of the precentral, premotor and arcuate areas in the rhesus monkey.


A comparison of the growth of various microorganisms in air spaces of the turkey head.


Characteristics of two ascending pathways which originate in spinal dorsal horn of Macauco mulatta.


The acute effect of aminoazobenzene and some of its derivatives on RNA polymerase activity in isolated rat liver nuclei.


Deficits in unsignaled avoidance behavior in rhesus monkeys asphyxiated at birth.


Decreased glucose tolerance in the rat following hypophysectomy.


Immunochemical studies of cystic fibrosis tissues: the detection of increased concentrations of an antigen which was identified as C-reactive protein.


The role of PMN-leukocyte lysosomes in tissue injury, inflammation and hypersensitivy. VII. Liberation of vascular permeability factors from PMN-leukocytes during 'in vitro' phagocytosis.


Amount, class and specificity of antibody to the lipopolysaccharide of Salmonella enteritidis after immunization by various schedules.


The cardiac auto-immune system. 3. Studies on the cytotoxicity of heart antibodies for pulsating rabbit and rat heart cells in tissue culture.


Changes in triiodothyronine kinetics produced by variations of serum thyroxine-binding globulin capacity in subjects without thyroxine.


Effect of HCG antiserum on ovulation and corpus luteum formation in the monkey (Macaca fascicularis).


Evidence for an effect of inulin on the peripheral utilization of ketone bodies in dogs. The rates of transport and oxidation of acetoacetate have been measured in seven anesthetized, pancreatectomized, ketotic dogs using a constant infusion of acetoacetate-3-(14)C. Control experiments were performed in 14 normal dogs. In addition to the acetoacetate-(14)C, the latter were infused at a constant rate with varying amounts of unlabeled acetoacetate so as to obtain a range of ketone transport (26-65 mumoles/min.kg) comparable with that observed in the diabetic dogs (21-41 mumoles/min.kg). The specific activities of acetoacetate and beta-hydroxybutyrate in blood became equal during the infusion of labeled acetoacetate, indicating that the net transport of acetoacetate represents that of total ketones. In each group, the concentration of ketones was an exponential function of the rate of transport, but for any value below 30 mumoles/min.kg, ketone concentration in the diabetic dogs was about 3 times that in normal dogs, indicating an impairment of mechanisms for utilizing ketones in insulin deficient animals. Maximal capacity to utilize ketones in diabetic dogs was slightly more than half that of normal ones. A similar fraction (32-63%) of the infused (14)C appeared in respiratory CO(2) in the two groups and was independent of the rate of transport. In seven of the normal dogs, administration of insulin and glucose increased removal of the infused ketones and increased the fraction of (14)C appearing in respiratory CO(2). These results demonstrate that utilization of ketones in extrahepatic tissues is influenced by insulin; impaired utilization contributes to diabetic ketosis and is probably essential to the production of severe ketoacidosis.


Cholesterol synthesis in the intestine of man: regional differences and control mechanisms. Cholesterol in the circulating serum pool is derived either from absorption of dietary cholesterol or from endogenous synthesis principally in the liver and gastrointestinal tract. While the control of intestinal cholesterogenesis has been elucidated in several lower animal species, no data currently are available in the case of man. In the present study using tissue specimens obtained by suction biopsy in 29 normal subjects, we have shown the rate of cholesterogenesis is low in the stomach (25 +/-6 mmumoles/g per 2 hr) and rectum (40 +/-8 mmumoles/g per 2 hr); in the small bowel the rate progressively decreases in the proximal duodenum (90 +/-16 mmumoles/g per 2 hr); distal duodenum (80 +/-11 mmumoles/g per 2 hr); and distal jejunum (35 +/-5 mmumoles/g per 2 hr); but abruptly increases in the distal ileum (280 +/-33 mmumoles/g per 2 hr). Indirect evidence is provided that the intestinal crypt epithelium is the main site of this sterol synthesis. Fasting for 48 hr suppressed the rate of cholesterogenesis in the distal duodenum from a control value of 80 +/-11 mmumoles/g per 2 hr to 40 +/-8 mmumoles/g per 2 hr while cholesterol feeding for 7 days did not alter the rate of cholesterol synthesis (75 +/-12 mmumoles/g per 2 hr). This resistance to cholesterol feeding also was present in the distal ileum where control and cholesterol-fed subjects had comparable rates of cholesterogenesis (280 and 261 mmumoles/g per 2 hr, respectively). Interruption of the enterohepatic circulation, in contrast, resulted in greatly enhanced sterol synthesis with a mean rate of 259 +/-29 mmumoles/g per 2 hr being found in the duodenum of four patients with biliary obstruction as compared with the rate of 80 +/-11 mmumoles/g per 2 hr in control subjects. These studies indicate that the mechanisms of control of cholesterol synthesis by the human intestine are similar to those described for the intestine of lower animals; this also appears to be true for the human liver. Thus, the marked differences in over-all cholesterol metabolism between various lower mammalian species and man cannot be explained by fundamental differences in control mechanisms; rather, these differences must reflect variations in some other parameter of cholesterol metabolism.


Hypogammaglobulinemia associated with accelerated catabolism of IgG secondary to its inteaction with an IgG-reactive monoclonal IgM. Hypogammaglobulinemia due to a new pathophysiological mechanism was studied in a patient with Sjögren's syndrome, a monoclonal IgM and a mixed (IgM-IgG) cryoglobulinemia. The IgM (IgMdk) component of the cryogel possessed light chains of lambda-type with highly restricted electrophoretic mobility analagous to those of a Waldenström's macroglobulin. IgMdk reacted specifically with native IgG, with IgG subclasses 1, 2, and 4, and with the Fc piece of IgG to form a cryogel. Serum concentrations of IgG 1, 2, and 4 were 10% of normal, whereas the IgG3 level was slightly increased and the IgM level was markedly increased. Viscosity and analytical ultracentrifugation studies with the purified mixed cryogel (IgM-LgG) indicated soluble complex formation over a temperature range (36-38 degrees C) attainable in vivo. Immunoglobulin turnover studies revealed a markedly elevated rate of IgM synthesis with a normal survival of IgM, IgA, and IgE. IgG3, which failed to form complexes with IgMdk at body temperature, had a normal synthetic rate and survival. In contrast, the other IgG subclasses showed reduced synthesis and shortened survival. These studies are the first indicating a short survival of some IgG subclasses with a normal survival of another. The hypogammaglobulinemia appears to be due in part to a new mechanism of accelerated protein catabolism: The rapid elimination of IgG due to its interaction with an IgG-reactive monoclonal IgM.


A quantitative method for determining human gamma G allotype antigens (Gm). I. Relatinship between Gm antigenic strength and phenotype.


The mechanism of indction of cold agglutinins by mycoplasma pneumoniae.


Antigenic analysis of human heart tissue. Further characterization of an organ-specific antigen of heart tissue.


Murine thyroiditis: importance of adjuvant and mouse strain for the induction of thyroid lesions.


Role of C5 in the nephritis of NZB-W mice.


Conversion of cholera exo-enterotoxin (choleragen) to natural toxoid (choleragenoid).


Serum group-specific (Gc) protein concentrations in patients with carcinoma, melanoma, sarcoma, and cancers of hematopoietic tissues as determined by radial immunodiffusion.


The clinical significance of the optic strut.


The clinical management of the diabetic adolescent.


Gynecology and obstetrics.

Gynecology and obstetrics.


Study of the defibrination syndrome associated with acute hepatic failure.


Optimization of radioimmunoassay for human growth hormone by the charcoal-dextran technique.


Scanning electron microscopy of red cell agglutination.


Human erythrocyte membranes: effect of lipid extraction on binding of IgG ("warm") autoantibodies and Rh isoantibodies.


Detection of serum hepatitis virus carriers by testing for the SH (Australia) antigen. A review of current methodology.


Schistosoma mansoni and S. haematobium: homocytotropic reagin-like antibodies in infections of man and experimental animals.


Two further examples of anti-Cob.

Two further examples of anti-Cob.


Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose.


Influence of the parental Rhesus types on the segregation of genetic abnormalities.


Density gradient centrifugation studies on lymphocytic choriomeningitis virus and on viral ribonucleic acid. Lymphocytic choriomeningitis (LCM) virus, Traub strain, was purified from BHK-21 tissue culture medium. The virus was then analyzed by equilibrium centrifugation and rate zonal centrifugation in sucrose gradients. A buoyant density in sucrose of 1.18 g/ml was found and the S(20, w) value was estimated to be about 470 to 500S. Furthermore, the (3)H-uridine-labeled ribonucleic acid (RNA) from virus was extracted from LCM virus and analyzed by rate zonal centrifugation. Two major and one minor single-stranded RNA components were found with sedimentation coefficients of 28, 22, and 18S.


Sodium citrate orally for painful sickle cell crises.


WHO Expert Committee on Drug Dependence. Eighteenth report.


Experimental alpha chymotrypsin glaucoma studied by scanning electron microscopy.


Differential diagnosis in Mikulicz's syndrome, Mikulicz's disease, and similar disease entities.


Comparison of electrophoretic mobility of tear lysozyme in 50 subjects.


Experimental studies on the effective procedure to inhibit the development of tolerance to and dependence on morphine.


Blood concentration method in the diagnosis of Chagas disease.


The action of insulin on glucose uptake by isolated rat soleus muscle. I. Effects of cations.


The action of insulin on glucose uptake by isolated rat soleus muscle. II. Dissociation of a priming effect of insulin from its stimulatory effect.


Threonine metabolism in a strain of Bccillus subtilis: enzymic oxidation of the intermediates D-and L-lactate.


Ankylosing spondylitis and its variants. Edioorial comment.


Radiotherapy for patients with tumours of the testicle.


The limbic system in relation to vision.


Contribution of John F. Fulton to the study of cerebellar function.


Influence of ovarian status and intrauterine devices on the diamine oxidase and histamine content of sheep endometrium.


Animal model in biomedical research.

Animal model in biomedical research.


Distribution of blood groups in southern Orissa.


Ossifications and fracture-healing in paraplegia and brain injuries.


Urinary acidification and electrolyte excretion in renal hypertensive rats.


Intestinal commensals and parasites of the South African baboon (Papio cynocephalus).


The role of RhoGAM in therapeutic and spontaneous abortion.


WHO expert committee on leprosy.

WHO expert committee on leprosy.


Introduction--Biosatellite 3 results.

Introduction--Biosatellite 3 results.


Analysis of 10 minutes of physiological data from the Biosatellite 3 from lift-off to orbital insertion.


Sleep-wake activity patterns of a Macaca nemestrina monkey during nine days of weightlessness.


Circadian rhythms of the Macaca nemestrina monkey in Biosatellite 3.


Sleep and wake states in the Biosatellite 3 monkey: visual and computer analysis of telemetered electroencephalographic data from earth orbital flight.


Digital computer analysis of neurophysiological data from Biosatellite 3.


Cardiovascular observations of the Macaca nemestrina monkey in Biosatellite 3.


Further studies on the uptake of Synkavit and a radioactive analogue into tumour cells in tissue culture. In a previous paper, the exact conditions under which the radioactive drug 2-methyl-6,7-ditritio-1,4-naphthaquinol bis disodium phosphate could be selectively incorporated into HEp/2 cells were reported. This work has now been extended and suggests that the selective property associated with two human tumour cell lines established in culture, HEp/2 and HeLa, and two forms of mouse ascites tumour cells propagated in vivo, is a metabolic conversion of the drug (priming stage) to a form which can probably be freely incorporated by all cell types. It is suggested that the observed variations in uptake of label with changes in pH, cell concentration and the inorganic phosphate concentration of the medium indicate that the "priming" stage is critically dependent on the conditions of the experiment.Work with the non-radioactive analogue, Synkavit, indicates that under conditions where the drug is incorporated selectively into cells, incubations in excess of 20 minutes cause a large percentage of the population to lose its reproductive integrity.


Early protection by vaccines in burns.

Early protection by vaccines in burns.


Studies on a phytohemagglutinin from the lentil. II. Multiple forms of Lens culinaris hemagglutinin.


Studies on the extracellular alkaline phosphatase of Micrococcus sodonensis. II. Factors affecting secretion.


Amino acid sequences of tryptic peptides of cytochromes b5 from microsomes of human, monkey, porcine, and chicken liver.


Isolation and characterization of the peptides derived from soluble human and baboon skin collagen after cyanogen bromide cleavage.


Glycans from streptococcal cell walls. Immunological and chemical properties of a new diheteroglycan from Streptococcus faecalis.


Turnover of the cell wall of Gram-positive bacteria.


The glucagon-sensitive adenyl cyclase system in plasma membranes of rat liver. I. Properties.


The action of 'terramycin' on the primary exoerythrocytic development of Plasmodium vivax and Plasmodium cynomolgi ceylonensis.


The glucagon-sensitive adenyl cyclase system in plasma membranes of rat liver. IV. Effects of guanylnucleotides on binding of 125I-glucagon.


Medical uses of radiation.

Medical uses of radiation.


Evaluation of doxepin hydrochloride (Sinequan).


Regulation of chromosome replication in Bacillus subtilis: marker frequency analysis after amino acid starvation. Marker frequency analysis of DNA isolated from amino acid-starved Bacillus subtilis cells shows that most chromosomes have not completed replication to the terminus. This finding agrees with earlier results concerning replication after amino acid starvation in this organism. The results are not compatible with regulation of chromosome replication at the initiation step only, and they suggest that a second regulatory circuit controls replication under conditions of amino acid starvation.


Red-green cone interactions in the increment-threshold spectral sensitivity of primates. Threshold spectral sensitivity of primate eyes is the upper envelope of the sensitivity of three response channels. Sensitivity in the green and red channels is modeled as linear difference functions reflecting neural interaction between cones containing photopigments with 535- and 575-nanometer peaks. Sensitivity in the blue channel seems determined by a single class of cones containing a 445-nanometer photopigment.


The isolation of a large Babesia species and other blood parasites from British cattle.


The occurrence of agglutinating, complement-fixing, and incomplete antibodies to Brucella abortus in milk, and their relationship to the milk ring test.


Activation of epidermal tyrosinase.

Activation of epidermal tyrosinase.


Stimulation of pituitary glucose oxidation by thyrotropin-releasing hormone.


Actions of prostaglandin F2-alpha on the splenic vascular and capsular smooth muscle in the dog. 1. The actions of prostaglandin F(2alpha), which is known to be released by the spleen on sympathetic nerve stimulation, have been investigated in the isolated blood perfused spleen of the dog.2. Low arterial concentrations of F(2alpha) (less than 10 mug/100 ml) caused marked reductions in splenic vascular resistance.3. High arterial concentrations of F(2alpha) (above 10 mug/100 ml) caused increases in splenic vascular resistance.4. Little action on splenic volume was observed by any arterial concentration of F(2alpha).5. The reductions in splenic vascular resistance caused by low arterial concentrations of F(2alpha) were reversed by blocking doses of phenoxybenzamine.6. No appreciable interaction between F(2alpha) and the splenic responses to sympathetic nerve stimulation, adrenaline and noradrenaline was observed.7. The role of the prostaglandins released by the spleen is discussed.


Contrast pleurography in rhesus monkeys (Macaca mulatta).


Surgical management of motility disturbances of the esophagus and cardia.


Effect of disinfectants and drying on the virus of hemorrhagic enteritis of turkeys.


Comparison of Transgrow and T-M media for Neisseria meningitidis surveys.


Infusion of protein hydrolysates in the newborn infant: plasma amino acid concentrations.


Oxygen therapy for premature infants.

Oxygen therapy for premature infants.


Spontaneous uterine activity in nonpregnant primates (Macaca mulatta).


Immunization against Marek's disease.

Immunization against Marek's disease.


Tetracyclines and permanent teeth: the relation between dose and tooth color.


Oral manifestations of systemic genetic disorders. 3.


Injuries to the adolescent athlete.

Injuries to the adolescent athlete.


Mg plus plus- and Ca plus plus-induced platelet aggregation and ADP.


Gamma M globulin and thrombasthenic platelets.


Immunological identification of two urokinases.


Immunological identification of three urokinases.


Coagulation studies in 45 cases of nephrotic syndrome without uremia.


The essential fatty acids.

The essential fatty acids.


Arterial composition and metabolism: esterified fatty acids and cholesterol.


Intellectual performance of differentially reared chimpanzees. 3. Oddity.


Consumer views.

Consumer views.


Clinical and experimental considerations infacial bone biology: growth, remodeling and repair.


Facial skeletal changes after mandibular condylectomy in the adult monkey.


Selection of cells transformed to prototrophy for sporulation markers. A method is presented for reproducible quantitative selection of recombinants for spore markers in transformation analysis.


Transport of aromatic amino acids by Pseudomonas aeruginosa. Kinetic studies of the transport of aromatic amino acids by Pseudomonas aeruginosa revealed the existence of two high-affinity transport systems which recognized the three aromatic amino acids. From competition data and studies on the exchange of preformed aromatic amino acid pools, the first transport system was found to be functional with phenylalanine, tyrosine, and tryptophan (in order of decreasing activity), whereas the second system was active with tryptophan, phenylalanine, and tyrosine. The two systems also transported a number of aromatic amino acid analogues but not other amino acids. Mutants defective in each of the two and in both transport systems were isolated and described. When the amino acids were added at low external concentrations to cells growing logarithmically in glucose minimal medium, the tryptophan pool very quickly became saturated. Under identical conditions, phenylalanine and tyrosine each accumulated in the intracellular pool of P. aeruginosa at a concentration which was 10 times greater than that of tryptophan.