Tetracycline levels in bronchial secretions. The excretion of tetracycline HCl in sputum was studied in 42 hospital patients suffering from various respiratory disorders who were receiving oral tetracycline therapy of 1.0 gram daily in divided doses.The tetracycline levels were estimated biologically by a cup-plate agar diffusion method using B. cereus. Two separate methods of sputum homogenization are described: enzymic liquefaction with pronase, a powerful proteolytic enzyme, and ultracentrifugation at 103,000 g for three hours which sediments the viscous mucoids leaving 80-90% as a clear, non-viscous supernatant. The two methods allowed duplicate assays on sputum samples and generally showed close agreement. The ultracentrifugation technique is favoured for normal purposes because of its simplicity and the avoidance of any biochemical interference.A close correlation was shown between the tetracycline levels in sputum samples and the average serum level for each patient. Over the serum tetracycline range 1.0-5.0 mug/ml, the mean sputum tetracycline levels after centrifugation were 23-18%, and the average level was 0.50 mug/ml.The levels found in sputum were compared with the minimum inhibitory concentration of tetracycline for strains of H. influenzae and Strep. pneumoniae. Eighty-six percent of 29 H. influenzae strains tested and all Strep. pneumoniae strains tested were sensitive to 0.5 mug/ml tetracycline. The remaining H. influenzae strains had a minimum inhibitory concentration of 1.0 mug/ml or higher, a tetracycline level in sputum which appeared to be outside the mean range at the oral dosage of 1.0 gram daily. Hence it is suggested that a small proportion of patients with lower respiratory tract infections due to H. influenzae would be unresponsive, or only partially responsive, to this usual therapeutic range of tetracycline.


Trimethoprim as an additional selective agent in media for the isolation of N. gonorrhoeae. The incorporation of trimethoprim into existing selective media using either saponin-lysed blood agar or chocolate agar as the base is suggested for the routine isolation of the gonococcus. The principal advantage over other selective media is the inhibition of Proteus mirabilis and other commensal organisms as a consequence of which multiple swabs can be plated out onto a single selective plate irrespective of source, while the recovery rate of the gonococcus from these specimens is increased. The relative merits of both basic media are briefly discussed.


Serum-alpha2-macroglobulin, transferrin, albumin, and IgG levels in preeclampsia. A radial immunodiffusion technique has been used to measure levels of four serum proteins in preeclampsia with or without proteinuria and in normal pregnant and non-pregnant controls. In preeclampsia unaccompanied by proteinuria, albumin and transferrin levels are similar to those found in the normal pregnant controls, but there are significant falls in alpha(2)-macroglobulin and IgG. When preeclampsia is accompanied by proteinuria there is a marked fall in albumin and an increase in alpha(2)-macroglobulin. Since alpha(2)-macroglobulin has antiplasmin activity it is possible that increased levels of this protein in preeclampsia accompanied by proteinuria contribute to the intravascular coagulation which has been described in this disorder.


The role of L-glutamine in the phenotypic change of a rod mutant derived from Bacillus subtilis 168.


The euglobulin lysis time test: an ineffectual monitor of the therapeutic inhibition of fibrinolysis. In two clinical situations associated with hyperfibrinolysis the administration of antifibrinolytic drugs resulted in clinical haemostasis. The dilute clot lysis time and fibrin plate activity test but not the euglobulin lysis time reflected this control of excessive fibrinolysis by the antifibrinolytic drugs. The inhibition by epsilon-amino-caproic acid (EACA) of hyperfibrinolysis induced in vitro was reflected by the dilute clot lysis time but not by the euglobulin lysis time. Paper chromatography of the supernatant and euglobulin fractions as prepared for the euglobulin lysis time test from plasma with added EACA demonstrated that some 85% of the EACA was present in the supernatant, normally discarded during the test. Similarly, cellulose-acetate electrophoresis of the supernatant and euglobulin fractions from plasma containing Trasylol demonstrated the drug in the supernatant only. These findings indicate that when acetic acid is added to plasma containing EACA or Trasylol only a small proportion of the drug is precipitated with the euglobulin fraction. The euglobulin lysis time is thus an inaccurate index of the neutralization of hyperfibrinolysis by antifibrinolytic drugs.


The isolation and characterization of mutants of Bacillus subtilis and Bacillus licheniformis with disturbed morphology and cell division.


Studies on the antigenic specificity of Mycobacterium leprae. I. Demonstration of soluble antigens in leprosy nodules by immunodiffusion.


Reversion of Bacillus subtilis protoplasts to the bacillary form induced by exogenous cell wall, bacteria and by growth in membrane filters.


Sequential loss of loci in thymine-starved Bacillus subtilis 168 cells. Evidence for a circular chromosome.


Loss of DNA behind the growing point of thymine-starved Bacillus subtilis 168.


The relation of serum parathyroid hormone to serum calcium and magnesium levels.


The production of antilymphocytic serum.


Determination of erythrocyte carbonic anhydrase B and C: an aid in the diagnosis of thyroid disorders?


Antilymphocytic serum--its constitution, effect and mode of action.


Diamine oxidase (histaminase) and heparin inhibition of gastric secretion in man.


Ontogenetic variation of serum esterases in the horse.


Bovine lipomatosis.

Bovine lipomatosis.


The effect of urethane on newborn calves.


Two unique features of the anterior cerebellum in the higher primates, including man.


A study of the substrate-binding site in hog kidney diamine oxidase.


Regulation of competence development in Bacillus subtilis.


A sex-limited serum protein variant in the mouse: inheritance and association with the H-2 region.


Buoyant densities of DNA of mammals.

Buoyant densities of DNA of mammals.


Related base sequences in the DNA of simple and complex organisms. V. The specificity of interactions between oligonucleotides and denatured DNA.


Treatment of gonorrhoea with three different antibiotic regimes: doxycycline 300 mg., procaine penicillin plus benzyl penicillin 2.4 m.u., benzyl penicillin 5 m.u. plus probenecid.


Further studies of penicillin resistant gonococci.


Related base sequences in the DNA of simple and complex organisms. VI. The extent of base sequence divergence among the DNAs of various rodents.


Effect of excision and benzimidazole treatment on folate content of wheat leaves and wheat leaf chloroplasts.


Listeriosis--rare or rarely recognized.

Listeriosis--rare or rarely recognized.


Placental weight in human foeto-maternal incompatibility.


Proper place of intrauterine transfusions in management of fetuses with Rh hemolytic disease.


Cerebral gigantism. Triad of findings helpful in diagnosis.


New ideas and data on competence and DNA entry in transformation of bacillus subtilis.


Involvement of intracellular phospholipase C in autolytic fragmentation of cytoplasmic membranes of Bacillus cereus.


Anchorage of collagenous fibrils in brain surface wrapping basal membrane (membrana limitans gliae superficialis).


Induction of interferon in chick cells by adenoviruses of different origin.


Enhancement of reovirus infectivity by extracellular removal or alteration of the virus capsid by proteolytic enzymes.


A simple immunologic method for the quantitation of immunoglobulin G in human cerebrospinal fluid.


[Studies for the determination of clinically available criteria of resistance of tubercle bacilli to rifampicin].


Widespread ectopic ossification following head injury.


Quantitative evaluation of the changes in serum prealbumins in myocardial infarction.


Ethosuximide blood levels in epileptics.


Results of treatment with ethambutol and capreomycin in chronic pulmonary tuberculosis.


Antibodies to serum lipoprotein antigens and Australia-SH antigen in multiply transfused patients.


Fibrinolytic activity and haemagglutination inhibition immunoassays.


Jaw orthopedic and orthodontic treatment for cleft lip and palate patients in the Uppsala region of Sweden.


After-treatment of burns-grafted and spontaneously healed--in particular a trial of aliderm leo ointment.


Immunodiffusion tests in patients with Schistosoma mansoni infection.


Familial nephropathy associatdd with retinitis pigmentosa, cerebellar ataxia and skeletal abnormalities.


Lung bacterial clearance in the burned rat.


The prevention of erythroblastosis.

The prevention of erythroblastosis.


Ultramicroscopical appearance of Pneumocystis carinii.


Outbreak of infantile gastro-enteritis caused by Escherichia coli O114.


Atypical IgA with hidden light chain.

Atypical IgA with hidden light chain.


An electrophoretic and immunologic study of Murex trunculus haemocyanin.


The conversion of tyrosine to catecholamines and the biogenesis of N-acetyl-dopamine in isolated epidermis of the fiddler crab, Uca pugilator.


A controlled comparison of psychiatric day center treatment with full-time hospitalization: interim report.


Further studies of aminorex, a new anorexigenic agent.


The value of a salicylate-free analgesic following dental surgery.


Absorption and tolerance of clindamycin-2-palmitate in infants below 6 months of age.


Interaction between catabolic and anabolic steroids.


The production of platelet thrombi.

The production of platelet thrombi.


Relationship between competence and doubling time in Bacillus subtilis cultures.


Two forms of ATP sulfurylase in Furth mouse mastocytoma.


Illness following accidental self-inoculation of Brucella abortus strain 19 vaccine.


Post-myocardial infarction pain in a series of male patients in the Albury-Wodonga district.


Rhesus incompatibility--an assessment of the hazards of amniocentesis, and the effect of liquor volumes on amniocentesis prediction.


In vitro assay of cell-mediated immunity: the inhibition of migration of sensitized human lymphocytes by HL-A antigens.


Allergic encephalomyelitis: immunologically specific inhibition of cellular passive transfer by encephalitogenic basic proteins.


Waldenström's macroglobulinaemia with anti-IgG activity: a series of five cases.


Sub-units of the first complement component in immunologic deficiency syndromes: independence of Cls and Clq.


Immunofluorescent studies of thyroid auto-antibodies in chickens of the obese strain (OS).


Occurrence of Listeria antibodies in select occupa- tional groups.


Unexpected extent of immunochemical cross-reactions between rabbit and human serum proteins.


The effect of bursectomy, thymectomy and x-irradiation on the incidence of precipitating liver and kidney auto-antibodies in chickens of the obese strain (OS).


Trachea-specific antigens in normal and malignant human tissues.


Quantitation of the fourth complement component by electrophoresis in agarose gel containing antibodies.


Microtechnique for quantitative evaluation of in vitro lymphocyte transformation.


Isolation and properties of mycoplasma from the respiratory tract of sheep with jaagsiekte in Kenya.


Precipitin reactions in monkeys (Macaca sinica) experimentally infected with Toxocara canis and in children with visceral larva migrans syndrome.


Effect of experimentally altered occlusal vertical dimension on temporomandibular articulation.


Tissue reactions to polymer-coated Vitallium pin implants.


Nervous system injury and the whole body.


Birth order as a patient variable in military psychiatry.


Motivation therapy with the aging geriatric veteran patient.


The patient-corpsman within a milieu therapy setting: a further approach to the manpower shortage.


Some postnatal aspects of intrauterine transfusions.


Case for diagnosis.

Case for diagnosis.


Theoretical and practical approach to drug research analysis.


Coded system for computer retrieval of pathology diagnoses.


Reduction of carious lesions at the margins of restorations.


Errors in tissue pO2 determinations.

Errors in tissue pO2 determinations.


The Alaskan native teenager: his health in a boarding school.


Heat stress--a strategic military factor.


Standardization of blood transfusion reaction studies in the military; delegation of responsibility for a medical team concept; role of the Hospital Transfusion Board.


The approach to the oliguric patient.

The approach to the oliguric patient.


Medical support of the Mobile Riverine Force, Vietnam.


Memoirs of a medical P.O.W.

Memoirs of a medical P.O.W.


Acceptance of rubella vaccination in a military dependent population.


Circulating immonoglobulin M: increased concentrations in endemic and sporadic goiter. Increased concentrations of immunoglobulin M have been found in the circulation of approximately half of patients with either endemic or sporadic nontoxic goiter. Blood was obtained from patients in several iodine-deficient goitrous areas; the patients with sporadic goiter resided in or about New York City. Concentrations of immunoglobulins G, A, and D were normal. Blood for control purposes was taken from patients residing in cities near the goiter areas where there was no iodine deficiency, and in New York City. Most of these samples came from hospitalized patients without known thyroid disease and were collected at random. Chi-square values for the difference between the number of goitrous patients with elevated concentrations of immunoglobulin M and those in the control patients were highly significant statistically.


C-reactive protein in serum from infants as determined with immunodiffusion techniques. II. Infants with various infections.


Analytical techniques for cell fractions. 18. Use of cellulose wicks to monitor agglutination reactions.


Identification of mycobacteria--what constitutes adequate examination?


The distribution of optic nerve fibers in Macaca mulatta.


The release of acetylcholine and 5-hydroxytryptamine from the mesencephalon of the unanesthetized rhesus monkey.


Comparison of glomerular filtration rate measurements using inulin, 51CrEDTA, and a phosphate infusion technique.


L-leucine sensitive, heat-stable alkaline-phosphatase isoenzyme detected in a patient with pleuritis carcinomatosa.


Thyroid iodoproteins in vertebrates: ultracentrifugal pattern and iodination rate.


Comparative properties of some histidases.


Localization of central cardiovascular control mechanism in the brain stem of the monkey.


Effect of brain iso-antibodies on learning and memory in the rat.


Recurrent inhibition and facilitation in the monkey, Macaca mulatta, in relation to spinal shock.


Role of aflatoxin in Indian childhood cirrhosis. (A preliminary communication).


Gargoylism. (A case report).

Gargoylism. (A case report).


Nurses' guide to central venous pressure monitoring.


The successful control of malaria in the 173d Airborne Brigade.


Field medical problem. The possible medical evacuation of a soldier to the Zone of Interior(ZI).


Calculous disease in Vietnam: observations on labile hyperuricemia.


Myoclonus epilepsy with Lafora bodies: case report with electron microscopic observation.


Embolization of an indwelling venous catheter.


Evaluation of a new method in teaching medical mathematics to Navy hospital corps school students.


The effects of oral magnesium oxide on renal calcium excretion.


Bridging the gap.

Bridging the gap.


Military outcome of trainees served by an Army Mental Hygiene Consultation Service.


Peripheral nerve injuries studied by evoked potential recordings.


Spontaneous pneumothorax: a ten-year study.


The AMA and military medicine.

The AMA and military medicine.


Remarks of the Federal Medical Chiefs.

Remarks of the Federal Medical Chiefs.


Inhalation therapy: experiences in two army general hospitals.


Developments in hemodialysis in the Veterans Administration.


Congenital absence left lobe of the liver associated with gastric ulcer: report of a case.


Perforated ameboma of the transverse colon: a case report.


Conversion hysteria versus malingering: a personal confession.


Injuries of the penile and bulbous urethra.


Field medical problem. Recommendations concerning mess personnel hired from local villages.


Splenosis: report of a case.

Splenosis: report of a case.


Auto-oxidation of thioridazine hydrochloride and trifluoperazine dihydrochloride: implications for clinical practice.


Infectious mononucleosis in the Armed Forces.


Annual meeting American Military Surgeons. Presidential address.


Changing attitudes in the management of leprosy.


The role of military psychiatry in the development of community mental health centers.


Some considerations in the operation of a field medical laboratory system.


The results of treatment of lung cancer by surgery, radiation and chemotherapy at a USPHS hospital.


Viral hepatitis: studies of the hepatitis associated antigen.


Fat embolism in Vietnam battle casualties in hemorrhagic shock.


Inter-American efforts for animal disease control.


Mammals of South Vietnam. I. Mammals commonly trapped during surveys.


Mammals of South Vietnam. II. Disease implications.


Medical evacuation in the Ryukyu Islands.


Ovarian and tubo-ovarian abscess in pregnancy: report of a case and a review of the literature.


Brigadier General Roger Brooke, MC, USA (1878-1940).


Case for diagnosis.

Case for diagnosis.


Reading disability in the armed services.


Pneumococcal meningitis.

Pneumococcal meningitis.


The epidemiology of illness in naval environments. I. Illness types, distribution, severities, and relationship to life change.


The epidemiology of illness in naval environments. II. Demographic, social background, and occupational factors.


Mycoplasma pneumoniae pneumonia: clinical course and complications.


Diving physiology and decompression illness.


Late-onset slowly progressive sex-linked recessive muscular dystrophy.


Amebic liver abscess: use of percutaneous catheter drainage.


Serratia marcescens endotoxin shock in the dog.


The control of dental caries.

The control of dental caries.


Maxillofacial injuries: survey of 8,746 cases.


Intrauterine contraception and the Armed Forces.


Field medical problem. Veterinary staff officer assigned to the 80th Medical Brigade.


Suicide prevention in the military.

Suicide prevention in the military.


Current status of prophylaxis of meningococcal disease.


Evaluation of an automated method for blood grouping in the military service: a system analysis.


A practicable classification of the injured for computer simulation studies.


Management of urethral injuries in war casualties.


Another enemy in Vietnam.

Another enemy in Vietnam.


Foveomacular retinitis: the relationship to stress.


Group therapy for basic trainees: a means of coping with adjustment problems.


Death in military police custody and confinement.


Coast Guard psychiatric inpatients: assessment, management and disposition.


A salt water aspiration syndrome.

A salt water aspiration syndrome.


Male Turner's syndrome: a case report with normal external genitalia, hormonal assays and secondary sex characteristics.


Calcified cyst of the adrenal gland.

Calcified cyst of the adrenal gland.


Vascular complications of the thoracic outlet syndrome: a case report.


Filariasis without microfilaremia: a case report.


Case for diagnosis.

Case for diagnosis.


Trauma to the gastrointestinal tract.

Trauma to the gastrointestinal tract.


Progress in the Federal Medical Services.


Military experience with ulcer disease: a review.


The Armed Services Whole Blood Processing Laboratory (ASWBPL), McGuire AFB, N.J.


Biochemical changes observed in heat exhaustion under field conditions.


Clostridia septicemia in malignant disease.


Stokes-Adams attacks due to Mobitz II block in a patient with left axis deviation and right bundle branch block.


Toxicity vs purity in staphylococcal enterotoxin "B".


Neurilemmoma of the bronchus.

Neurilemmoma of the bronchus.


Pertinent criteria for electrodiagnostic and clinical evaluation of Bell's palsy.


Recurrent gallstone intestinal obstruction with bacterial enteritis proximal to the obstruction: a case report.


Field medical problem. Prepare a five-minute ARSTRIKE briefing.


A research concept for the interpretation of human missile wounds by the pathologist.


"Rifampin--now that the secret is out".

"Rifampin--now that the secret is out".


Rationale for the use of L-dopa in the torsion dystonias.


Drug-induced relief of experimental tremor in monkeys.


Locomotion and activity phasing of 6 carnivores and a monkey.


Localization of carbonic anhydrase acitivity in transporting urinary epithelia.


Chemical and bacteriological tests for determining susceptibility to, and activity of dental caries; a review.


Ultrastructural studies of feline leukemia virus.


Thermal denaturation of mineralized and demineralized bone collagens.


Echinococcosis: A preoperative consideration.


Collagen metabolism in skin and periodontal membrane of the marmoset.


The normal pericardium.

The normal pericardium.


Congenital cystic eye.

Congenital cystic eye.


New contact lens for peripheral fundus examination and photocoagulation.


Effects of optic nerve transection on intraocular pressure in monkeys.


Experience at the National Communicable Disease Center with micro serological techniques for the diagnosis of viral diseases.


Effects of dietary alterations on insulin secretion in normal individuals.


Iatrogenic disorders.

Iatrogenic disorders.


The role of automated blood grouping as an information retrieval system.


Health manpower: opportunities and problems in cross-over from the military to the civilian health economy.


Vascular complications of intracranial injury.


Observations on intensive coronary care at Walter Reed General Hospital.


Persistent sinus bradycardia treated by permanent transvenous pacemaker.


Dimensions of psychiatric illness among first-term enlistees in the United States Navy.


Epidemic meningococcal meningitis in Africa and the Middle East.


Smoking: insidious suicide and personal air pollution.


Acid reactivity in the distal esophagus.


Field medical problem. Planning dental support for a newly established communications zone in an active theater of operations.


The role of medical research.

The role of medical research.


Graduation address, Boston and Bethesda naval hospitals.


Clinical applications of serologic tests for systemic mycoses.


First isolation of Microsporum racemosum--Dante Borelli 1965 from Romanian soil. (New data on its pathogenic properties).


Effects of erythropoietin and testosterone on erythropoiesis in bone marrow of isolated perfused hind limbs of dogs.


Effect of uracil and 2-thiouracil on leucocyte production in the rat.


Homozygous hemoglobin D Punjab.

Homozygous hemoglobin D Punjab.


Catalase activity of immature and mature red cells from acatalasemic mouse mutant.


Specific, active, intradermal immunization against leukemia in guinea pigs.


Cytogenetic findings in acquired aplastic anemia.


Myelocyte proliferation in pernicious anaemia.


Haemolytic disease of the newborn due to ABO incompatibility. A new aid to diagnosis.


Transketolase activity of white blood cells.


The effects of hemorrhagic shock and pulmonary ischemia on lung compliance and structure in baboons.


Results of a clinical trial concerning the worth of prophylactic oophorectomy for breast carcinoma.


The second look operation for carcinoma of the ovary.


The renal distribution of sulfonamides.

The renal distribution of sulfonamides.


The interchangeability of stimulatory factors isolated from three microbial RNA polymerases.


Effect of divalent metal ions on the reversed-phase chromatographic separation of transfer ribonucleic acids.


Some observations on the effect of the concentration of ethylenediamine tetra-acetic acid (EDTA) on the packed cell volume of domesticated animals.


Hodgkin's disease and other malignant lymphomas. Systematic studies of the patterns of anatomic distribution, pathways of probable spread, and prognosis of the malignant lymphomas have been greatly aided by the development of new histopathologic classifications and the introduction of more sophisticated and precise diagnostic techniques, such as lymphangiography and laparotomy with splenectomy and retroperitoneal node biopsy. Concomitantly, megavoltage radiotherapy apparatus has made total-lymphoid radiotherapy feasible and practical, and the availability of a widening spectrum of chemotherapeutic agents has ushered in a new era of combination chemotherapy. Collectively, these diagnostic and therapeutic advances have already begun to yield a dramatic improvement in the prognosis of Hodgkin's disease and the other malignant lymphomas.


Demography of serum immunoglobulins: differences in IgG and IgM levels in two normal Mexican adult populations.


Analysis of cholinesterases of intact cat cornea, ciliary body, lens, and retina.


The decay kinetics of substances which leave the anterior chamber by bulk flow.


Strain variation in the components of the extensor apparatus of the finger during flexion and extension. A biomechanical study.


Effect of cervical sympathetic stimulation on accommodation in guanethidine-treated monkeys.


Regional vascular reactivity in the middle cerebral arterial distribution. An experimental study in baboons.


Virus-like particles in systemic lupus erythematosus.


Nursing problems associated with prolonged recovery following trauma.


Pneumoperitoneum associated with jejunal diverticulosis.


Predicting measures of motor performance from multiple cortical spike trains. Recordings have been obtained simultaneously from several, individually selected neurons in the motor cortex of unanesthetized monkey as the animal performed simple arm movements. With the use of comparatively simple quantitative procedures, the activity of small sets of cells was found to be adequate for rather accurate real-time prediction of the time course of various response measurements. In addition, the results suggest that hypotheses concerning the response variables "controlled" by cortical motor systems may well depend upon whether or not the temporal relations between simultaneously active neurons are taken into account.


Electron microscopy of squirrel monkey parotid glands.


The predictive value of amniotic fluid spectrophotometry.


Qualitative and quantitative analysis of the proteins in human amniotic fluid.


Study of the possibility of selection of the avirulent strain Rickettsia prowazeki E in cocultivation with a virulent strain of Rickettsia prowazeki in chick embryos.


Imidazole derivatives. IV. Synthesis and pharmacologic activity of oxygenated derivatives of imidazo(1,2-a)pyridine.


Polypeptides. XLVII. Effect of the pyrazole-imidazole replacement on the biological activity of thyrotropin-releasing hormone.


Antibacterial activity of nitrobenzofurans.


Chemistry of cephalosporin antibiotics. XX. Synthesis and biological properties of 3-acyloxymethyl-7-(2-(thienyl)acetamido)-3-cephem-4-carboxylic acid and related derivatives.


Acyl derivatives of 5-hydroxy-6,7-benzomorphans. Prodine congeners.


Total parenteral nutrition.

Total parenteral nutrition.


Simian bone disease: unrecognized rickets in rhesus monkeys.


The adaptation of two human coronavirus strains (OC38 and OC43) to growth in cell monolayers.


In vivo studies with a toxic fraction of Pseudomonas aeruginosa.


Chemotaxis of polymorphonuclear leukocytes by protein A of the staphylococcus.


Purification and further characterization of an "IBV-like" virus (coronavirus).


Secretion of prolactin and growth hormone by cultures of adult simian pituitaries.


Protection against St. Louis encephalitis and West Nile arboviruses by previous dengue virus (types 1-4) infection.


Fecal streptococci on barley and malt kernels and instant malted milk powder. Among 500 barley kernels, tested singly, 1.8% (9 kernels) were positive for fecal streptococci. Among 800 malt kernels, tested singly by the same procedure, 68% (540 kernels) were positive for fecal streptococci. In unsulfured and sulfured malts, 67 and 68% of the kernels, respectively, were positive for these bacteria. Fecal streptococci were also isolated by an enrichment procedure from two brands of instant malted milk powder sold in supermarkets. Seventy randomly picked isolates from malts were identified to be fecal streptococci, belonging probably to more than one species.


Development of a camel kidney cell strain and its use in virology. A strain of camel kidney cells was developed and carried in serial passages. The subcultures were slow-growing in the early passages and were composed of heterogeneous cell population. By the 35th passage, the growth rate increased, and more homogeneous cells, mostly of the epithelioid type, were seen. The cell strain was highly susceptible to West Nile, Sindbis, vesicular stomatitis, adeno, and vaccinia viruses, and also was susceptible to herpes simplex, rinderpest, measles, and canine distemper viruses.


New method for detecting bacteriocin production. Bacteriocin production can be easily detected by using both sides of the agar contained in a standard petri dish.


Survival of Neisseria gonorrhoeae in the mail. Cultures of Neisseria gonorrhoeae, which used Thayer-Martin slants as transport medium, survived at least 1 day in the mail.


Purification of alkaline phosphatase from guinea pig bone marrow.


Preparation and properties of a homogeneous aromatic L-amino acid decarboxylase from hog kidney.


Formation of three-dimensional structure in proteins. I. Rapid nonenzymic reactivation of reduced lysozyme.


A comparison of phosphorylase isozymes in the rabbit.


Physical, biochemical, and immunological properties of coliphage MS-2 particles. H (heavy) and L (light) MS-2 particles differ in density, absorption spectrum, and infectivity. Studies on their sedimentation, ribonucleic acid (RNA) content and infectivity, appearance under the electron microscope, ribonuclease sensitivity, and A-protein content failed to demonstrate any difference between the two particle types. Studies on the size, RNA content, and density of the capsid and two smaller coat protein components were also conducted. The antigenic relatedness of five different viral and subviral particles of MS-2 were studied by using immunodiffusion and neutralization. Capsids and the H and L viral particles were shown to be antigenically related, whereas the coat protein monomers and dimers were shown to be unrelated to the higher-molecular-weight particles.


Wholesomeness of irradiated food with special reference to wheat, potatoes and onions. Report of a joint FAO-IAEA-WHO Expert Committee.


Genetic mapping of a defective bacteriophage on the chromosome of Bacillus subtilis 168. A genetic marker responsible for the killing activity of PBSX, a defective phage carried by Bacillus subtilis 168, has been located on the bacterial chromosome. Two mutant strains of B. subtilis 168, which produced tailless phage particles upon mitomycin C induction, were shown to carry lesions, designated xtl-1 and xtl-2, which were linked by transformation and PBS1-mediated transduction to metC. The link-age relationship between xtl and adjacent auxotrophic markers was determined by three-factor PBS1 transduction, the suggested order of markers being argO 1 metA metC xtl.


Variability and differences in the structure of digital tactile corpuscles of Cercopithecus aetiops.


Migratory arthralgias and cutaneous lesions as confusing initial manifestations of gonorrhea.


The effect of coumermycin A on the meningococcal carrier state.


Genetic analysis of loboid-ophthalmoptera, a homoeotic strain in Drosophila melanogaster.


Enteric fevers in and around Madurai.

Enteric fevers in and around Madurai.


Growth inhibition of newborn rats by plasma of monkeys immunized against rat growth hormone.


ABO and Rh blood groups in relation to marital status and childlessness in blood donors.


Receptors for human gamma G globulin on human neutrophils. Cell surface receptors for human gammaG antibodies directed against bacterial antigens were demonstrated on human neutrophils using an in vitro bacteriocidal-phagocytic assay. These results were confirmed by adherence of sensitized erythrocytes to monolayers of neutrophils or monocytes. Erythrocytes sensitized indirectly with antibacterial gammaG antibodies after passive sensitization with bacterial antigens adhered to both neutrophils and monocytes. Erythrocytes sensitized directly with conventional anti-D gammaG antibodies adhered only to monocytes, while those sensitized with the hyperimmune anti-CD gammaG antibody Ripley adhered to both monocytes and neutrophils. Adherence of anti-Rh or antibacterial gammaG antibodies to monocytes and neutrophils could be inhibited by whole gammaG, myeloma globulins of the gamma(1) or gamma(3) subclasses, or Fc fragments, but not by Fab fragment. These results indicate that receptors for the Fc portion of human gammaG antibodies exist on both neutrophils and monocytes, and that gammaG antibodies differ in their ability to attach to these two cell types. Differences in the behavior of the gammaG antibodies studied may be related to differences in the density of antibodies on the erythrocyte surface and receptors on the phagocytic cells.


Osteopetrosis (Marble bone disease) of the temporal bone.


Transformation in Bacillus subtilis. A simple direct method.


Two family outbreaks of perianal cellulitis associated with group A beta-hemolytic streptococci.


Additional data on diagnosis and epidemiology of Listeria infections.


The question of stemlines in human acute leukemia. Comparison of cells isolated in vitro and in vivo from a patient with acute lymphoblastic leukemia.


Surface immunoglobulin-moieties on lymphoid cells.


Annulate lamellae in human parathyroid adenoma.


Ultrastructural studies on rat retina damaged by visible light.


Noradrenalin content and ultrastructure of adrenergic nerves of rabbit iris after sympathectomy and hypoxia.


Ultrastructural changes in the motor nerve terminals caused by beta-bungarotoxin.


Quantitative and qualitative changes of structure and ultrastructure of intestinal epithelia during incubation in vitro.


Growth of elementary blood vessels in diffusion chambers. I. Process of formation and conditioning factors.


Fibrosarcoma induced by alpha particle irradiation.


Photoelectric measurement of horizontal eye movements in monkeys.


Isolation of bacilysin and a new amino acid from culture filtrates of Bacillus subtilis. 1. Bacilysin, a labile dipeptide antibiotic that lyses growing staphylococci, was isolated from culture fluids of Bacillus subtilis by a process giving higher yields than those previously obtained. 2. The process involves adsorption on a cation-exchange resin and elution with aqueous trimethylamine, separation from neutral amino acids and glutamic acid by chromatography on DEAE-Sephadex at pH8.7 and separation from other neutral peptides by chromatography in aqueous propan-2-ol on Sephadex G-25. 3. A new amino acid, which is chemically related to bacilysin, was isolated from the fraction containing neutral amino acids. 4. Two substances that yield alanine on hydrolysis, in addition to bacilysin, were obtained from the neutral peptide fraction.


The structure of bacilysin and other products of Bacillus subtilis. 1. Mass spectra of the trimethylsilyl derivative and the methyl ester of the N-trifluoroacetyl derivative of bacilysin indicated that the antibiotic had a molecular weight of 270. Several peaks in the spectrum of the methyl ester were consistent with the presence of an N-terminal alanine residue in the molecule. 2. The proton-magnetic-resonance spectrum of bacilysin confirmed that the antibiotic contained an epoxide group and the spin-spin splitting of the protons of the epoxide group indicated that the side chain of the epoxycyclohexanone ring was attached at C-4 and was alphabeta to the keto group. 3. The formation of an alphabeta-unsaturated ketone on reduction of bacilysin with chromous chloride also showed that the epoxide was alphabeta to the keto group. 4. The optical-rotatory-dispersion curve of bacilysin showed a positive Cotton effect. On the assumption that the reversed Octant rule for alphabeta-epoxyketones was applicable this revealed the absolute stereochemistry and enabled a definitive structure to be assigned to the molecule. 5. Similar measurements showed that substance AA1, isolated from culture supernatants, was the C-terminal amino acid of bacilysin. 6. Hydrolysis of substance P2 with leucine aminopeptidase and the mass spectrum of the methyl ester of its N-trifluoroacetyl derivative showed that this substance was l-analyl-l-alanine. 7. These results are discussed in relation to the biogenesis of bacilysin.


Sporulation in Bacillus subtilis. Correlation of biochemical events with morphological changes in asporogenous mutants. A comparison was made of morphological changes and successive, mainly biochemical, marker events for sporulation in 14 asporogenous mutants. The morphological and biochemical sequences are linked so that arrested development in one is accompanied by corresponding effects in the other. Thus mutants that fail to produce both protease and antibiotic do not progress beyond stage 0, formation of alkaline phosphatase appears to be associated with the transition from stage II to stage III and glucose dehydrogenase with that from stage III to stage IV. Stage II mutants may produce ;pygmy' cells or other bizarre cell-division forms. The biochemical sequence is dependent in the sense that if the occurrence of any one event is blocked that of all the succeeding events is also blocked. This has implications for biochemical models that have been proposed to explain the temporal sequence observed in spore development.


Biochemistry of bovine lens proteins. 3. Chemical and physical properties of alpha-crystallin subunits.


Deoxyribonucleic acid methylase of mammalian tissues.


Cation accumulation during the anaerobic-aerobic transition in brain mitochondria.


Inhibition of nonphosphorylating electron transfer by zinc. The problem of delineating interaction sites.


Biosynthetic precursors of 30S and 50S ribosomal particles in Escherichia coli.


The incorporation of mevalonic acid into the N6-(delta 2-isopentenyl) adenosine of transfer ribonucleic acid in Lactobacillus acidophilus.


Streptococcal nucleases. IV. Some properties and specificities of the ribonuclease action of the B and D enzymes.


Chronic carriage of Australia antigen without symptoms after renal transplant.


Liquor bilirubin levels in normal pregnancy: a reassessment of early prediction of haemolytic disease. A comparison was made between chemically and spectrophotometrically determined concentrations of total bile pigment in the liquor amnii. For the period 16 to 26 weeks' gestation the upper limit of normal bile pigment to protein ratio was found to be 0.4. Levels above this would be required as an indication for intrauterine transfusion. In 110 cases of isoimmunization of pregnancy these criteria were applied in the diagnosis of severity of the condition. The ratio of the bile pigment to protein was used rather than a simple bile pigment measurement, and found to be valuable.Nevertheless, it is important to emphasize that fallacious high bilirubin readings may arise by using whatever method, particularly owing to bilirubin and other breakdown products of blood contaminating the liquor.


Diabetic ketoacidosis.

Diabetic ketoacidosis.


Localization of gamma crystallins in the developing lens of the rat.


A note on the use of plaque reduction to test serums for canine distemper antibodies.


Demethylchlortetracycline used in bacterial infections of ear, nose, and throat.


The primacy of prevention in the delivery of medical care.


Ion transport by the primate neonatal stomach.


Renal effects of uricosuric agents in the Cebus monkey.


Effects of tyramine on responses to and inactivation of histamine in aortic strips.


The effect of octanoate on the carbohydrate metabolism of myometrium from the pregnant rhesus monkey.


Immunological studies with rat testis: antigenic characterization.


Evaluation of methods for determining antibacterial activity of serum and urine after colistimethate injection.


Relative frequency of the Hurler and Hunter syndromes.


Primary drug-resistant tuberculosis. Report of an outbreak.


Studies of Lp-lipoprotein as a quantitative genetic trait. Sera from 11 individuals which were classified as Lp(a-) by direct gel diffusion and by absorption tests showed the presence of Lp(a) lipoprotein when the sera were concentrated 120-fold. This Lp(a) antigen was demonstrated by gel diffusion to be indistinguishable from Lp-lipoprotein isolated from known Lp(a+) sera. The Lp(a) preparations from sera classified Lp(a-) also showed electrophoretic mobility in immunoelectrophoresis and schlieren diagrams in ultracentrifugal analyses similar to Lp-lipoprotein from Lp(a+) sera. The proposal is made that observed individual variations in tests for the Lp-lipoprotein reflect a quantitative genetic trait and that it is likely that different individuals produce Lp-lipoprotein in widely varying amounts. The consistency of this proposal with certain previous observations on the Lp system is discussed.


Complementarity of RNA produced by enzymic transcription of native and denatured B. subtilis DNA. This paper describes the preparation and some of the properties of the RNA specimens synthesized with the aid of the RNA polymerase of E. coli by transcription of the following DNA templates: (a) undenatured B. subtilis DNA (yielding N-RNA); (b) separated strand fractions L and H isolated by chromatography of the denatured DNA on methylated albumin (yielding L-RNA and H-RNA, respectively). The study of the hybridization behavior of the various RNA products showed that N-RNA, though able to form hybrids with either strand, hybridized with H-DNA to twice as great an extent as with L-DNA. The transcripts of the separated L and H fractions exhibited complete specificity with respect to complexing: L-RNA hybridized only with L-DNA, H-RNA only with H-DNA.


Visual adaptation in monkey cones: recordings of late receptor potentials. The retinal cones of cynomolgus macaque monkeys show marked amounts of adaptation when the receptor potential is elicited by brief incremental stimuli presented against steady backgrounds of increasing intensity. The results can be accounted for by mechanisms of response compression, modified by the effects of photopigment bleaching, which together set the gain of the system at each background level, while also making the response nearly linear over a significant range of intensities above and below that of each adapting stimulus.


Visual discrimination of movement: midbrain or forebrain? Monkeys whose optic chiasm and forebrain commissures had been sectioned and control monkeys with only the optic chiasm cut were tested for interocular transfer of discriminations based on direction of movement. Only the control animals showed transfer to the untrained eye, which suggests that discrimination of movement, like pattern, is a function strongly dependent on the cortex.


Energy and epilepsy.

Energy and epilepsy.


Effects of lactulose and Lactobacillus acidophilus on the fecal flora.


Creatine phosphokinase activity in central nervous system disorders and infections.


The elusive gonococcus.

The elusive gonococcus.


Cephalexin in the treatment of gonorrhea.


The rapid diagnosis of gonorrhea.

The rapid diagnosis of gonorrhea.


The biochemical effects of the d, m, and a genes on pigment cell differentiation in the axolotl.


Protein patterns in monkey oviductal fluid before and after ovulation.


The uptake of Hg203Cl2 by the intestinal wall of the duodenal and ileal segment in vitro.


Abnormal social behavior in phenylketonuric monkeys.


Natural alpha-radioactivity concentrations in bone and liver from various animal species.


A study on the mode of administration of thyrotropin releasing hormone (TRH) in mice.


The regional organisation of special care for the neonate.


Design of a perinatal center.

Design of a perinatal center.


Ultrastructural and immunochemical characterizatiog of one case of IgD myeloma.


Supportive management of the sick neonate. Parenteral calories, water, and electrolytes.


Low molecular weight IgM-immunoglobulins in Waldenström's macroglobulinemia.


A spontaneous cleavage of the rabbit IgG.


Rh prevention programme in Edinburgh, Scotland.


Studies on the immunoglobulins of the mouse intestinal secretions.


Further observations on immunoglobulins of external secretions.


Research on the oral antityphoid immunization in mice and its mechanism of action.


The protective activity of various antigen complexes of typhoid and paratyphoid B vaccines: controlled field trials and laboratory studies.


Localization of the antibody combining sites of M-component with known antibody specificity.


Human IgA- and IgM-globulins for clinical use.


Sensitizing versus immunizing properties of inactivated measles vaccine.


Abnormal proteinuria in malignant diseases.


The new penicillins and cephalosporins.

The new penicillins and cephalosporins.


Effect of oxygen concentration on o-diphenol oxidase activity.


Nicking activity of an endonuclease. I. Transfer ribonucleic acid complex of Escherichia coli.


Kappa:lambda light chain ratio in IgG eluted from rheumatoid arthritis synovium.


Viral hepatitis and tests for the Australia (hepatitis-associated) antigen and antibody. "Australia" antigen has been shown to be closely associated with serum hepatitis. The presence of the antigen and its corresponding antiserum can be detected in human beings (and in certain primates) by a number of laboratory tests. This is of great potential importance to blood transfusion and similar services because detection and exclusion of blood donors carrying the antigen might significantly reduce the risk of hepatitis from transfusions and other procedures.In this paper the present state of knowledge of "Australia" or "hepatitis-associated" antigen is reviewed. The currently employed tests are described in detail and their use, interpretation and limitations are discussed. Though it appears from early studies that the application of routine screening tests to blood donors would only reduce the risk to recipients by less than 25%, the more sensitive tests becoming available may increase this percentage and it is recommended that where competent laboratory services are available steps should be taken to set up a scheme for testing donors-provided that the current limitations of such a scheme are clearly recognized.


Speckled antinuclear factor in African sera.


Seroepidemiology of malaria.

Seroepidemiology of malaria.


Heterogeneity among strains of Mycoplasma granularum and identification of Mycoplasma hyosynoviae, sp. n. Twelve filtrable, pleomorphic organisms isolated from swine joints and respiratory tracts had typical colonial and microscopic characteristics of mycoplasmas. They resisted penicillin and did not revert to cell wall-producing bacterial forms in media devoid of bacterial inhibitors. The morphological and growth characteristics of these mycoplasmas were similar to those described previously for Mycoplasma granularum. However, a new name, M. hyosynoviae, is proposed for them since they differed biologically, serologically, and electrophoretically from the prototype strain of M. granularum. M. hyosynoviae required sterols, was stimulated by gastric mucin, and metabolized arginine; however, it did not metabolize urea, ferment glucose, or reduce tetrazolium. The organism produced "film and spots" on horse serum-supplemented medium and produced alpha hemolysis of guinea pig and sheep erythrocytes; however, it did not digest serum, produce phosphatase, or hemadsorb guinea pig or swine erythrocytes. M. hyosynoviae was distinguished from three other swine mycoplasmas, M. granularum, M. hyorhinis, and M. laidlawii, by means of acrylamide gel electrophoresis, growth inhibition, metabolic inhibition, and immunodiffusion techniques. It was also serologically and electrophoretically distinct from 13 additional non-swine mycoplasmas which require sterols and metabolize arginine.


Observations on fevers of unknown origin in the Rephblic of Vietnam.


Spontaneous pneumothorax and carcinoma of lung: a case report.


Quality of patient care.

Quality of patient care.


Effect on temperature of biogenic amine infusion into hypothalamus of baboon.


Preliminary results on intralabyrinthine temperature changes in the vestibular labyrinth during caloric stimulation.


Adenovirus associated viruses.

Adenovirus associated viruses.


Summary of classification of animal viruses, 1970.


Baltimore uses inner city aides in a Tuberculosis Control Program.


On the determination of component molecular weights in complex protein mixtures by means of disc electrophoresis.


Demonstration of antibody on lymph-borne immunoblasts by bacterial adherence.


On the mechanism of action of bleomycin. Strand scission of DNA caused by bleomycin and its binding to DNA in vitro.


A new microbial product, oudenone, inhibiting tyrosine hydroxylase.


Peptidases in Escherichia coli K-12 capable of cleaving lysine homopeptides.


Mucopeptide metabolism during growth and sporulation in Bacillus megaterium.


Replication of insect viruses in bacterial hosts.


Laboratory tests for hepatitis.

Laboratory tests for hepatitis.


Status of investigative aspects of hemorrhagic shock.


Cervical spinal cord hemorrhages in experimental head injuries.


Diabetic ketoacidosis.

Diabetic ketoacidosis.


Changes in pulsatile coronary blood flow induced by coronary vasodilators.


Human transplantation antigens.

Human transplantation antigens.


Histopathologic and immunological studies on chronic renal lesions in rats induced by aminonucleoside of puromycin.


Chemical composition and antigenicity of a residual protein of Mycobacterium tuberculosis.


Aerospace toxicology. I. Propellant toxicology.


Changes in cerebrospinal fluid sodium and potassium concentrations during seizure activity.


Long-term treatment of Parkinson's disease with levodopa.


A three-year assessment of an action line method of timing intervention in rhesus isoimmunization.


Response of the rhesus monkey uterine cervix to chronic estrogenic stimulation. This study investigates the changes in the columnar epithelium of the endocervix of rhesus monkeys following chronic estrogenic stimulation.  4 ovariectomized animals were administered estrone daily and killed at 2, 4, 8, and 12 weeks of treatment; findings were compared to those from 2 ovariectomized animals which had been treated with only 4 injections of physiologic doses of estrone.  As had been reported by previous studies, results showed a great response of stratified squamous epithelium throughout the endocervical canal.  However, foci of squamous cells also were found scattered throughout the cervical canal of controls which were stimulated by physiologic doses, thus showing that squamous cells are normal components of the cervix; such areas, however, are difficult to recognize in unstimulated animals, but become recognizable after estrogenic stimulation.  It is probable that foci of stratified squamous epithelium are normal components of the cervix, and that the stratified epithelium which is formed during chronic estrogenic stimulation arises by the proliferation and extension of these areas.  There is no evidence for the origin of stratified squamous epithelium from columnar cells, and consequently no justification for the use of the term metaplasia with regard to the chronically estrogen-stimulated Rhesus cervix.


Changes in fetal acid-base chemistries following tromethamine (THAM) or bicarbonate infusions into fetal circulation or amniotic fluid.


Absence of active transmural sodium transport in primate neonatal bladder.


Spectrophotometric analysis of amniotic fluid in early gestation.


Design and evaluation of a system for thermal decontamination of process air.


Application of ultrafiltration for enzyme retention during continuous enzymatic reaction.


Synthesis and biological activity of thyrotropin-releasing hormone.


Measurement of TSH in plasma and pituitary of the rat by a radioimmunoassay utilizing bovine TSH: effect of thyroidectomy or thyroxine administration on plasma TSH levels.


Circhoral oscillations of plasma LH levels in the ovariectomized rhesus monkey.


Effects of gonadectomy on plasma LH concentration in the rhesus monkey.


The three thyroxine-binding proteins in rat serum: binding capacities and effects of binding inhibitors.


A family study of spondyloepiphyseal dysplasia.


Intra-articular thio-tepa compared with depomedrol and procaine in the treatment of arthritis.


Chronic pseudomonas osteomyelitis. Report on the use of gentamicin sulphate in three cases.


Arthrography of the ankle joint. Experience in one hundred seven studies.


Lateral rotatory dislocation of the ankle without fracture. A case report.


Isolated dislocation of the medial cuneiform bone--a rare injury of the tarsus. A case report.


Microbiological assay with increased sensitivity for chlortetracycline in eggs.


The surgical route.

The surgical route.


Radioimmunoassay for thyrotropin (TSH) in serum from baboons.


Effects of clomiphene on estrogen induced changes in plasma proteins in monkeys.


Inhibition of growth of Bacillus thuringiensis by amino acids in defined media.


Biographical variables and post-hospitalization adjustment of psychiatric patients in the Naval Service.


Entomology of the gastrointestinal tract: a brief review.


Alternobaric vertigo in military divers.


United States Medical Programs in South Vietnam.


Case for diagnosis.

Case for diagnosis.


Tinea nigra palmaris.

Tinea nigra palmaris.


The treatment of manic-related diseases with lithium carbonate.


Attitudes of non-career dentists serving in the US Army: Report of a survey.


Post-traumatic acute acalculous cholecystitis in young males.


Seat belt injuries: a review and report of four cases.


Trends in medical education.

Trends in medical education.


Jejunal carcinoma as the source of recurrent gastrointestinal hemorrhage.


Meningococcal infection.

Meningococcal infection.


Some aspects on immunity to Akiba caulleryi infection in chickens.


Fibrin proteolysis in the monkey uterine cavity: variations with and without IUD.


Physical, chemical, and serological studies on carnation mottle virus.


Joint FAO-WHO Expert Committee on Milk Hygiene. Third report.


Partial characterization of the complementary sites involved in the reaction between adenovirus type 7 and erythrocyte receptors.


Requirements of ascorbic acid, vitamin D, vitamin B12, folate, and iron. Report of a Joint FAO-WHO Expert Group.


Experiemental and clinical studies on muramidase (lysozyme). I. Muramidase activity of normal human blood cells and inflammatory exudates.


A new abnormality of platelet function.

A new abnormality of platelet function.


Ketonaemia in uncontrolled diabetes mellitus. Blood ketone bodies, serum insulin levels, and plasma free fatty acids were examined in a series of patients with "non-ketotic diabetic coma" and compared with the findings in ketoacidotic subjects. Serum insulin levels in six "non ketotic" patients ranged between 1 and 25 muu./ml. and were not significantly different from levels reported in patients with ketoacidosis. In addition, plasma free fatty acids were shown to be unrelated to the degree of ketonaemia. The investigation shows that neither the levels of serum insulin nor those of free fatty acids can explain the absence of hyperketonaemia in some cases.


Influence of the chronic metabolic acidosis on the motility of the small intestine (experimental data).


Desynchronization of the excitatory process in the alpha-motoneurons and its role for the performance of motor actions.


Effect of repetitive infant-infant separation of young monkeys.


The double diffusion precipitin test in human fascioliasis. The recent outbreaks of human fascioliasis in Britain provided the opportunity for employing the double diffusion precipitin test in the diagnosis of this disease. In sensitivity and specificity this test appears to equal the complement-fixation test and has the advantages of simplicity and speed in its performance.


Reduced interferon response in mice congenitally infected with lymphocytic choriomeningitis virus.


The influence of the indigenous bacterial flora upon the interferon response of mice.


Separation of adenovirus penton base antigen by preparative gel electrophoresis.


Serological relationship of strains of tobacco necrosis virus and their ability to activate strains of satellite virus.


Comparison of adenovirus types 2 and 5 hexons by immunological and biochemical techniques.


Synthesis of virus macromolecules in L-929 cells infected with Mayaro virus.


Mastocytoma in the third eyelid of a dog.


Ribonucleic acid-dependent deoxyribonucleic acid polymerase in visna virus. A ribonucleic acid-dependent deoxyribonucleic acid polymerase was found in virions of visna virus. The enzyme product was resistant to ribonuclease and alkaline hydrolysis but susceptible to the digestion of deoxyribonuclease.


Diformyl diaminodiphenyl sufone (DFD) as an antimalarial in man.


A four-year study of the internship in United States Air Force hospitals: an objective measurement of gain in clinical competence.


Skin sensitization potential of the riot control agents BBC, DM, CN and CS in guinea pigs.


Congenital absence of the right pericardium associated with herniation of the right atrium.


Antibiotic sensitivities of shigella isolates in Vietnam, 1968-1969.


Occult thyroid carcinoma and the cold nodule.


Seizures in infectious mononucleosis.

Seizures in infectious mononucleosis.


Marihuana associated psychosis in Vietnam.


Ulcer disease and the dilemma of the disability boards.


An electronic data processing program for the Air Force cytology screening project.


Aqueductal stenosis and hydrocephalus after mumps encephalitis.


Myocaddial sarcosporidiosis in the camel.


Illicit drug use and addiction in the United Staes.


Health of Papago Indian children.

Health of Papago Indian children.


Search for a preparation to prevent venereal diease and pregnancy.


Bat and soil studies for sources of histoplasmosis in Florida.


Relation between annoyance reactions and attitude to source of annoyance.


Reliability and validity of survey data on physical health.


Survey of differences in cost of diets of anemic and nonanemic children.


Mortality rates for rheumatic fever and rheumatic heart disease, 1940-65.


Food poisoning outbreak in Kentucky traced to creamed turkey.


Rabies control on Guam.

Rabies control on Guam.


Survey of changes in food habits during pregnancy.


Teaching programs in the epidemiology of mental health.


Ultrastructural pathology of glomerular lesions in gnotobiotic mice with congenital lymphocytic choriomeningitis (LCM) virus infection.


Studies on the cell wall of Spirillum serpens. 1. Isolation and partial purification of the outermost cell wall layer.


Relation of calcium to mucosal structure and vitamin B12 absorption in the canine intestine.


Studies on diphosphopyridine nucleotidase and platelet damaging factor in an extracellular product of Listeria monocytogenes.


The characterization of fluorescent skin diphtheroids.


Analysis of the thermal transition curves of deoxyribonucleic acid from microorganisms.


Proflavine binding and release by sensitive and resistant Bacillus subtilis.


Cadmium as a trace element and cadmium binding components in human cells.


Canine secretory immunoglobulins: identification of secretory component.


Interspecies "transfer" of delayed hypersensitivity in vitro with RNA extracts.


Immunochemical studies on the interaction between staphylococcal protein A and gamma-G globulin.


Immune hemolysis and the functional properties of the second (C2) and fourth (C4) components of complement. I. Functional differences among C4 sites on cell surfaces.


The in vitro effects of goat antisera to rabbit thymocyte subcellular fractions.


C3 in high molecular weight complexes: separation from plasma of nephritic patients and normal subjects by a method that avoids in vitro artifacts.


Studies of common baldness in the stumptailed macaque. IV. In vitro metabolism of testosterone in the hair follicles.


Evaluation of the phototoxic potential of topically applied agents using long-wave ultraviolet light.


Interaction of visual and nonvisual effects in lateral geniculate nucleus and area striata in primates.


Light and electron microscopical observations on the laminar distribution of axon terminals in the lateral geniculate nuclus.


Binocular interaction in monkey visual cortex.


Spontaneous consumption coagulopathy in a dog with thyroid cancer.


Ultrastructural features of plasma cells in patients with paraproteinemias.


Redirected aggression and gonadal hormones in captive Rhesus monkeys (Macaca mulatta).


Pseudoparaproteinemia due to hypertransferrinemia.


Aggression and gonadal hormones in captive Rhesus monkeys (Macaca mulatta).


Blood group and secretor status in tropical sprue.


Purification of the delta toxin os Staphylococcus aureus.


Studies on MSH-induced melanogenesis: effect of long-term administration of MSH on the melanin content and tyrosinase activity.


Serum insulin and growth hormone response to hemorrhagic shock.


Experimental formation of goiter in exencephalic fetal rats subjected to maternal hypervitaminosis A.


Thyroxine-protein interactions. VII. Effect of thyroxine analogs on the binding of 125-I-thyroxine to highly purified human thyroxine-binding globulin.


Freeze-dried composite tendon allografts: an experimental study.


A method of "immunocentrifugation" in acrylamide-sucrose linear density gradients.


Symposium. Myeloma. d. Fine structure of human myeloma cells and macroglobulin-producing cells.


Effect of vitamin D2 and of ergosterol peroxide on deamination of mono- and diamines in liver mitochondria and on the activity of highly purified preparations of mitochondrial monoamine oxidases.


Ocular effects of argon laser radiation. I. Retinal damage threshold studies.


The correlation between conformation and biological activity of gramicidin S analogs.


Multiple forms of supernatant malate dehydrogenase in salmonid fishes.


Properties of carbonic anhydrase isozymes isolated from porcine erythrocytes.


The amino acid neighbors of thyroxine in thyroglobulin.


Amniotic fluid proteins in normal and Rh-sensitized pregnancies.


Effect of oestradiol 3-benzoate on the biosynthesis of egg proteins by cod.


Wax retraction as a technique for compensating the effect of surface irregularities in high-energy radiotherapy.


The composition of the cyst wall of the beet cyst-nematode Heterodera schachtii. 1. Cyst walls of the beet cyst-nematode (Heterodera schachtii Schmidt) were obtained by sieving a suspension of crushed cysts; about 15mg of dried cyst walls was obtained from 1000 cysts. 2. The cyst walls contained 68% protein calculated from nitrogen content. Glutamic acid, glycine, proline and hydroxyproline made up about 54% by weight of the amino acids obtained on acid hydrolysis. 3. Minor constituents of the cyst wall were hexosamine (3.3%), lipid (6%), carbohydrate (2%) and phenols (2%). The hexosamine was identified as galactosamine. 4. The cyst walls contained inorganic material (ash 17%), most of which was extractable with EDTA, but not with water. Major inorganic components were calcium and phosphorus (1.7% and 1.5% respectively, by weight). Carbon dioxide (about 1% by weight) was liberated from the cyst walls on acidification. 5. The cyst walls of H. schachtii and the potato cyst-nematode (Heterodera rostochiensis) contained different amounts of the same amino acids. They also differed in their inorganic content and in the nature of the hexosamine present.


A comparison of the response of tumour and normal tissue in the mouse exposed to single doses of fast neutrons or electrons.


Neurofibromatosis of the renal artery.

Neurofibromatosis of the renal artery.


Variation in the position of the central axis maximum build-up point with field size for high-energy photon beams.


Scanning electron microscopic studies of the canal of Schlemm.


Pupil size and ocular pressure after sympathectomy and prostaglandin-catecholamine treatment.


Fibrinolytic activity in the aqueous humour.


An ocular parasympathetic nerve pathway of facial nerve origin and its influence on intraocular pressure.


Cellular uptake of some amino acids and amines in vitro into rabbit and monkey anterior eye segment preparations.


Electrophoretic and immunologic differences between monoclonal IgM molecules and their subunits in native plasma.


Immunochemical studies on a laminaribiosyl-azoprotein conjugate.


Purification of an organ-specific thermostable antigen found in bovine brain.


Anti-thymocyte serum effects on Plasmodium berghei infection in rats.


In vitro adhesion of eosinophils to infective larvae of Wuchereria bancrofti.


Histaminase.

Histaminase.


Enzymatic synthesis of mannosyl-1-phosphoryl-decaprenol by a cell-free system of Mycobacterium tuberculosis.


Streptokinase-activatable proactivator of human and bovine plasminogen.


Diagnosis of typhoid fever by haemagglutination.


Relation of schistosomiasis to typhoid fever.


Chronic urinary salmonella carriers with intermittent bacteraemia.


Pathogenesis of chronic salmonella bacteremia.


Australia antigen and lepromatous leprosy studies in South India and elsewhere.


Treatment of S. typhi--paratyphi A urinary carriers.


Changing pattern of enteric fevers in U.A.R.


Methods for comparing the cariogenicity of foodstuffs.


Round table discussion on public health control--critique for future research.


The pathology of salmonelloses--a review.


Comparative efficiency and application of blood culture techniques for enteric fever diagnosis.


Enterica of childhood.

Enterica of childhood.


Unusual presentation of enteric infections.


Widal and blood picture in the diagnosis of typhoid fever.


Statistics and epidemiology of typhoid fever in UAR.


The use of ferrous sulphate for dosimetry by post.


Energy calibration points for clinical electron accelerators.


Widening a palate by combined surgical and orthodontic procedures. Case report.


The mixed lymphocyte tumor cell culture and the fluorescein diacetate cytotoxicity test. Two useful methods for the demonstration of cell mediated tumor specific immunity in man.


The effect of packed cell volume, hemoglobin content and red cell count on whole blood viscosity.


Leucocytosis following administration of cryoprecipitate.


Trypsin activity of haemophilic blood.

Trypsin activity of haemophilic blood.


Congenital hypoprothrombinemia. Case report.


Haemorrhagic disease due to hereditary deficiency of factor XIII. Description of a family.


New water disinfectant: an insoluble quaternary ammonium resin-triiodide combination that releases bactericide on demand. Strongly basic anion-exchange resins form stable, water-insoluble combinations with triiodide ions. The combinations have remarkable antibacterial properties: 3.0 x 10(5)Escherichia coli cells per ml were killed when passed through a 3.8-g column of commercially available resin treated with triiodide (volume 4 ml after treatment). In an attempt to deplete the resin-triiodide complex, 1.14 x 10(9)E. coli cells in 15 liters were passed through the column with no significant loss of effectiveness. The antibacterial capabilities of the resin-triiodide columns ranged from 10(6)Salmonella typhimurium per ml to 1.1 x 10(4)Streptococcus faecalis per ml. Staphylococcus aureus and Pseudomonas aeruginosa were also tested and killed at concentrations of 1.8 x 10(4) and 1.3 x 10(5) per ml, respectively. The cells were not filtered from the water. They emerged from the column in nonviable form. This was demonstrated by using (14)C-labeled bacteria. The irreversible nature of the antibacterial action was revealed when attempts to wash the damaged cells did not restore viability.


Paper chromatographic techniques for the determination of cephalothin and desacetylcephalothin in body fluids. Paper chromatographic procedures may be used to detect cephalothin and its metabolite desacetylcephalothin in urine, plasma, synovial fluid, and cerebrospinal fluid. Protein-bound antibiotics are released from plasma, cerebrospinal fluid, and synovial fluid by dilution with an equal volume of dimethylformamide. Data are presented on the sample preparation, paper chromatographic system, and other specific techniques.


Effect of pH on the activity of erythromycin against 500 isolates of gram-negative bacilli. Erythromycin was found to be a more effective inhibitor of gram-negative bacilli in alkaline medium than in neutral or acid medium. A definite effect was noted with all of 500 recent clinical isolates of Escherichia coli, Klebsiella-Enterobacter, Pseudomonas aeruginosa, and Proteus mirabilis studied, but it was most striking with E. coli. At pH 8.5 all strains, except for 14% of those of P. mirabilis, were inhibited by concentrations of erythromycin readily achieved in urine with common therapeutic doses.


Adsorption of formaldehyde by various surfaces during gaseous decontamination. Study of the effect of atmospheric relative humity (RH) on the adsorption of paraformaldehyde-generated formaldehyde gas on various surfaces and the effect of the adsorbed formaldehyde on the death rate of bacterial spores showed that increasing the RH caused a corresponding increase of formaldehyde levels on all surfaces. The amount peaked at 83% RH. The levels obtained at 100% RH were slightly below those at 83% RH. Cotton cloth had a much greater affinity for the gas at all RH than either glass or stainless steel. The death rate of bacterial spores on surfaces containing adsorbed formaldehyde was high for the first hour after removal from the formaldehyde atmosphere but decreased rapidly thereafter. This held true for both cotton and glass surfaces. Also, formaldehyde levels of 15 to 27 mug/ml of nutrient broth caused inhibition of bacterial growth, but levels above 27 mug/ml rendered broth sterile.


Comparison of methods for removal of nonspecific inhibitors of arbovirus hemagglutination. Human sera were treated with kaolin, acetone, and dextran sulfate to determine the best method for removing nonspecific hemagglutination inhibitors. Results indicated that on surveys for group A, group B, and some group C arbovirus HI antibodies, dextran sulfate treatment of sera could be used effectively. This method, however, has limited usefulness for detecting HI antibody for a number of arboviruses, particularly some members of the Bunyamwera supergroup since nonspecific inhibitors for these antigens were not completely removed. HI antibodies in sera drawn early after dengue and Venezuelan equine encephalitis infection were detected more readily after dextran sulfate treatment than after kaolin treatment. Kaolin, but not dextran sulfate, was shown to remove antibody from IgM fractions of sera.


Aflatoxin B1 induction of lysogenic bacteria. A technique for biological verification of aflatoxin B(1) was developed based on toxin-mediated induction of lysis in a lysogenic strain of Bacillus megaterium NNRL B-3695. Reduction of culture turbidity was determined at various concentrations of toxin. Incubation of 1.1 x 10(-4) g (dry weight) of cells/ml of growth medium containing 25 mug of B(1) per ml at 37 C reduced initial turbidity 0.20 absorbance units in 4 hr. If the bacterial lysate of the lysogenic strain, after a 2-hr incubation with 25 mug of B(1) per ml, was plated with a sensitive B. megaterium strain (NRRL B-3694), plaque-forming units increased approximately 150 times relative to the control. Comparable testing of the effects of aflatoxin on the nonlysogenic, sensitive strain demonstrated that 75 mug of B(1) per ml neither induced lysis nor plaque-forming units. Although induction is not an exclusive property of aflatoxin B(1), the differential response of the lysogenic and sensitive Bacillus strains to B(1) offers a unique and rapid technique for biological verification of the toxin.


Sterility test method for petrolatum-based ophthalmic ointments. A sensitive sterility testing procedure for the detection of microbial contamination in petrolatum-based ointments is described. The method involves dissolving the ointment in filter-sterilized isopropyl myristate and filtering through a membrane filter. Improved sensitivity is obtained by blending the membrane in Trypticase Soy Broth before incubation. Filter-sterilized isopropyl myristate is shown to be less toxic to microorganisms than heat-sterilized isopropyl myristate. The isopropyl myristate method is more sensitive than the polyethylene glycol-ether method for the detection of microbial contamination.


Microcount method for petrolatum-based topical ointments containing waxes. A practical solvent system for the detection of microorganisms in topical ointments has been developed. The method involves dissolving 0.5 g of topical ointment in 50 ml of a solvent mixture (92 parts isopropyl myristate, 6 parts carbon disulfide, and 2 parts xylene) and filtering it through a 0.45-mum membrane filter. Residual solvent is then washed from the filter pad with 200 ml of sterile 0.5% Brain Heart Infusion broth containing 0.1% Tween 80. The filter pad is then removed and placed on a petri plate containing Trypticase Soy Agar medium. The petri plates thus prepared are then incubated at 37 C for 7 days, and the colonies produced are then counted. The toxicity of the solvent mixture was determined against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella newington, and spores of Bacillus subtilis and was found to be less toxic than the heat-sterilized isopropyl myristate and comparable to the filter-sterilized isopropyl myristate.


Ethylene oxide resistance of nondesiccated and desiccated spores of Bacillus subtilis var. niger hermetically sealed in various polymeric films. The resistance to destruction of spores of Bacillus subtilis var. niger hermetically sealed in various polymeric films and exposed to ethylene oxide with and without relative humidity was determined. The effect of desiccation was also determined. The order of increased resistance to sterilization with regard to type of polymeric film was found to be: polyethylene equal to polyvinyl chloride, less than nylon, less than cellophane/polyethylene laminate, less than phenoxy, less than mylar/polyethylene laminate. Desiccated spores sealed in various polymeric films were much more resistant to ethylene oxide sterilization than nondesiccated spores. Relative humidity was an important factor in ethylene oxide sterilization with spores not sealed in polymeric films. However, with spores hermetically sealed in polyethylene, added relative humidity was an insignificant factor in the sterilization process.


Dry-heat destruction of Bacillus subtilis spores on surfaces: effect of humidity in an open system. Bacillus subtilis var. niger spores were tested for dry-heat resistance on stainless-steel strips hung in an oven. Heat resistance was dependent on the relative humidity before and during treatment, which in turn affected the water content of the spores. Higher humidities increased the heat resistance of the spores. D-values ranged from 16.1 min for spores conditioned at <2% relative humidity (RH) and treated at 0.34% RH to 37.6 min for spores conditioned at 89% RH and treated at 1.1% RH. The y-intercept of the regression line ranged from 6.94 x 10(4) for spores conditioned and treated at the low humidities to 2.00 x 10(5) for spores conditioned at 89% RH and treated at 0.34% RH. For a constant value of N(0), the y-intercept appears to be lowered by low-humidity conditions. The statistic log y(0)/log N(0) is used to measure the downward displacement of the regression line. Values obtained in this experiment range from 0.90 for spores conditioned at <2% RH and treated at 0.34% RH to 1.04 for spores conditioned at <2% RH and treated at 1.1% RH. A combination of linear regression and analysis of variance methods was used for data analysis. The former estimates D-values and y-intercepts, whereas the latter is sensitive to differences between treatments.


The effects of histamine in malaria.

The effects of histamine in malaria. 1. Extracts of the blood of monkeys (Macaca mulatta) infected with Plasmodium knowlesi contain histamine. A mean total concentration of 0.15 mug/ml was present in the circulating blood.2. No histamine was detected in the blood of healthy monkeys.3. Vasodilatation and increased vascular permeability was observed at the site of injection of the extract into the skin and brain of guinea-pigs.4. Infiltration of the dermal layer by leucocytes was observed after intradermal injection of the histamine extract. A similar response was obtained in the brain.5. The extract of the blood from the infected animal produced hypotension in rabbits when administered intravenously.6. The pathophysiological significance of histamine in malaria is discussed.


Probability distribution of drug-resistant mutants in unselected populations of Mycobacterium tuberculosis. The fluctuation test shows that Mycobacterium tuberculosis mutates to resistance to isoniazid, streptomycin, ethambutol and rifampin spontaneously and at random. The average mutation rates for the drugs, in the same order, were calculated to be 2.56 x 10(-8), 2.95 x 10(-8), 10(-7), and 2.25 x 10(-10) mutation per bacterium per generation. The relatively high mutation rate to ethambutol resistance and the low mutation rate to rifampin resistance were confirmed by analyzing the increase in the proportion of mutants with time in a growing population of the tubercle bacilli. The highest proportions of mutants to be expected in unselected populations of the tubercle bacilli were calculated from the results of fluctuation tests.


4'-Demethyl-epipodophyllotoxin thenylidene glucoside (VM 26), a podophyllum compound with a new mechanism of action.


Comparative evaluation of commercial precipitating antisera against human IgM and IgM.


The effect of alkylation on the antimicrobial activities of 7-hydroxy- and 4-hydroxycoumarins.


Immunological reactivity of fowl ferritin.


Recent advances in avian endocrinology. 2. Inherited autoimmune thyroiditis in the fowl.


Schizogony of Plasmodium knowlesi in the presence of normal and immune sera.


Resistance of mice and rats to Babesia rodhaini challenge after inoculation with irradiated B. rodhaini infected red cells.


Resistance tests for triatomid bugs, vectors of Chagas's disease.


Immunological studies in tropical splenomegaly syndrome.


Immunization against Schistosoma japonicum in chimpanzees by administration of x-irradiated cercariae.


Anopheles (Kerteszia) cruzi, a natural vector of the monkey malaria parasites, Plasmodium simium and Plasmodium brasilianum.


Antigens and antibodies associated with Plasmodium falciparum infections in West Africa.


Trypanosomes of primates in Indonesia.

Trypanosomes of primates in Indonesia.


Instrument specifications for endoscopy of the equine upper respiratory tract and eustachian tube diverticulum.


Optic glioma. Clinical features and treatment.


Some comparative studies on the mammalian corneal epithelium.


Failure to demonstrate monoamine oxidase inhibition by glyceryl trinitrate in vivo.


Effects of lactate and other metabolites on food intake of monkeys.


Hyperinsulinemia in tube-fed hypophysectomized rats bearing hypothalamic lesions.


Effect of storage of Mueller-Hinton agar plates on zone sizes for antimicrobial testing. The length of time Mueller-Hinton agar plates can be stored at 4 C without affecting the size of zones of inhibition in susceptibility testing by the Bauer-Kirby method was studied. It was found that these plates can be stored for 3 weeks at 4 C without an appreciable affect on zone sizes. Storage of plates in sealed plastic bags did not alter the results significantly. The findings indicate that commercially prepared Mueller-Hinton agar plates, which may be several days old when received at the laboratory, are suitable for use in routine susceptibility tests by the Bauer-Kirby method.


Agar overlay plaquing technique for Pseudomonas aeruginosa in HeLa monolayers. The solid agar overlay procedure used for viral plaquing was adapted to the study of Pseudomonas aeruginosa plaques in HeLa monolayers. After adsorption of the organism to the HeLa monolayers, an overlay consisting of 10% newborn calf serum (NBCS), 90% Eagles basal medium (EBM), and 1.5% agar containing neutral red was added. Plaques developed after 24 hr of incubation at 37 C which were indistinguishable from viral lesions. Optimal conditions for plaques included: serum in the adsorption fluid as well as the overlay medium; 1-hr adsorption time; healthy HeLa monolayers; and the presence of EBM. The formation of plaques instead of colonies in agar was due to the inhibitory effect of NBCS or EBM. By omitting both of these components from the overlay, colonies appeared in place of plaques. Adult bovine serum acted in a similar bacterial colony-suppressing fashion, but fetal or agamma calf serum did not. Furthermore, NBCS or adult bovine serum when incorporated in the overlay medium instead of fetal or agamma calf serum displayed a plaque-suppressing effect by reducing the number and size of plaques formed. The findings lend further evidence to support the hypothesis that P. aeruginosa may produce plaques from a protected intracellular site within HeLa cells.


Bacteriophage active against the lactic acid beverage-producing bacterium Lactobacillus casei. A virulent bacteriophage which causes a decrease in acid production during fermentation of a lactic acid beverage named Yakult with Lactobacillus casei was isolated from the abnormal fermentation tank and named PL-1. L. casei S strain was the exclusive host cell among 18 lactic acid bacteria tested. The plaque was round with an average diameter of about 0.5 mm. It exhibited serological cross-reaction with previously isolated J1 phage. Under an electron microscope, the phage had a spermatozoon shape, with an icosahedral head (63 nm) and a long tail (12.5 by 275 nm) with about 55 striae. The free phage particles were stable at pH 5 to 8. The phage was quite sensitive to ultraviolet irradiation or to heating (60 C, 5 min), and the host was more sensitive than the phage to these treatments. Many kinds of antimicrobial chemicals were also phagocidal. Calcium ion (5 mm) was specifically essential for the phage growth cycle. A one-step growth experiment under optimum conditions (37 C and pH 6.0) showed that the eclipse period was about 75 min, that the latent period was 100 min after the phage infection, and that the average burst size was about 200. The possibility of arresting phage development in lactic acid fermentation is discussed.


Resistance of Bacillus subtilis spores to inactivation by gamma irradiation and heating in the presence of a bactericide. I. Suitability of viable count procedures. A statistical evaluation of viable count procedures utilized for obtaining treatment survival curve data for Bacillus subtilis NCTC 8236 spores is described. Within the various recovery conditions tested, incubation on nutrient agar containing 1% dextrose for 48 hr at 37 C was found to promote the highest count of viable spores surviving a variety of bactericidal treatments involving gamma irradiation, heat, and chlorocresol. The count of viable spores on the medium was not significantly altered when the dextrose was added to the nutrient agar either before autoclaving or aseptically at 50 to 55 C from a solution sterilized by filtration. The volume of medium which promoted the highest count of viable spores was 20 ml per 85 mm of diameter in disposable plastic plates. Counts of viable spores were reproducible on successive batches of media. The carry-over of variable concentrations of chlorocresol into the medium from serial dilutions affected the count of viable spores. Spores in the aqueous stock suspension used for all experiments were uniformly distributed after shaking and did not diminish significantly in viability after 16 months of storage at 5 C. Grouping of indexes of dispersion, calculated from quintuplicate plate colony counts, indicated that the suitability of the viable count procedures, employed for the enumeration of spores surviving the various bactericidal treatments, tended to diminish as the level of spore inactivation exceeded 95%.


Resistance of Bacillus subtilis spores to inactivation by gamma irradiation and heating in the presence of a bactericide. II. Factors affecting rates of inactivation by phenolic bactericides. Aqueous suspensions of Bacillus subtilis NCTC 8236 spores, surviving 150,000 or 300,000 rad of gamma irradiation under air from a cesium-137 source, exhibited an enhanced rate of inactivation, compared to nonirradiated spores, when heated with different phenolic bactericides. The apparent magnitude of the enhanced inactivation rate, observed from survival curves, increased progressively with the irradiation dose applied and diminished progressively as the severity of heat treatment with 0.2% chlorocresol was increased either by raising the temperature from 70 to 90 C or reducing the pH from 8 to 6. The enhanced inactivation rate was unaffected when the concentration of sodium chloride added to 0.2% chlorocresol was altered from 0.4 to 0.8%. The enhancement effect was also observed when the heat treatment was carried out with 0.5% phenol and 0.3% m-cresol.


The ecology of the atypicalmycobacteria.


Studies on the incorporation of labelled sulphate into cells and cell-free extracts of Nitrosomonas europaea.


The action of o-dihydric phenols in the hydroxylation of p-coumaric acid by a phenolase from leaves of spinach beet (Beta vulgaris L.). 1. Under defined conditions, the hydroxylation of p-coumaric acid catalysed by a phenolase from leaves of spinach beet (Beta vulgaris L.) was observed to develop its maximum rate only after a lag period. 2. By decreasing the reaction rate with lower enzyme concentrations or by increasing it with higher concentrations of reductants, the length of the lag period was inversely related to the maximum rate subsequently developed. 3. Low concentrations of caffeic acid or other o-dihydric phenols abolished this lag period. With caffeic acid, the rate of hydroxylation was independent of the reductant employed. 4. Hydroxylation was inhibited by diethyldithiocarbamate, but with low inhibitor concentrations hydroxylation recovered after a lag period. This lag could again be abolished by the addition of high concentrations of caffeic acid or other o-dihydric phenols. 5. Catechol oxidase activity showed no lag period, and did not recover from diethyldithiocarbamate inhibition. 6. The purified enzyme contained 0.17-0.33% copper; preparations with the highest specific activity were found to have the highest copper content. 7. The results are interpreted to suggest that the oxidation of o-dihydric phenols converts the enzymic copper into a species catalytically active in hydroxylation. This may represent the primary function for the catechol oxidase activity of the phenolase complex. The electron donors are concerned mainly, but not entirely, in the reduction of o-quinones produced in this reaction.


The effect of immunosuppression on viral encephalitis, with special reference to cyclophosphamide.


Immuno-electrophoretic characterization of brucella abortus antigenic strain 99. II. Chemical extracts.


Estimation of diethylcarbamazine (hetrazan).


Health of the pig reared in confinement.


Restraint of monkeys for clinical examination and treatment.


Veterinary radiotherapy.

Veterinary radiotherapy.


Prolongation of barbiturate anesthesia by chloramphenicol in laboratory animals.


Accidental lead poisoning in a rhesus monkey (Macaca mulatta).


Leech (Dinobdella ferox) infection of a Taiwan monkey (Macaca cyclopis).


A study of Klebsiella infections in owl monkeys (Aotus trivirgatus).


Studies on the distribution of blood types among various racial tribes in Formosa.


Methods of evaluating fetal distress.

Methods of evaluating fetal distress.


Synthesis of ribosomal RNA on a protein-DNA complex isolated from bacteria: a comparison of ribosomal RNA synthesis in vitro and in vivo.


Pulmonary tuberculosis with noncommunicating chest wll abscess.


Interaction between insecticides and soil. Microbiological assay of Sevin and Meta-iso-systox using bacteria as test organisms.


Decreased granulation tissue reaction after installment of hyaluronic acid.


Metabolism of thalidomide in Pseudomonas aeruginosa NCTC A 7244.


Zymograms in agar gel of some animal and bacterial proteinases.


Gel precipitation reactions between measles antigens and sera of patients with multiple sclerosis.


The K antigens of Escherichia coli. Re-examination and re-evaluation of the nature of L antigens.


Host-parasite relationships in experimental airborne tuberculosis. IV. Early events in the course of infection in vaccinated and nonvaccinated guinea pigs.


General term for mycobacteria other than mycobacterium tuberculosis.


Healing of esophageal anastomoses.

Healing of esophageal anastomoses.


Aspects of bacterial antagonism between Neisseria meningitidis and Bacillus subtilis.


The microbiology of gingival-dental plaque recent findings from primate research.


Treatment of malignant tumours with high doses of nitrogen mustard (TS 160) and-or high doses of ionizing radiation under the protection of autologous bone marrow (evaluation after a period of 7 years).


6-(p-hydroxyphenylazo)-uracil: a selective inhibitor of host DNA replication in phage-infected Bacillus subtilis. The azopyrimidine, 6-(p-hydroxyphenylazo)-uracil, inhibits the replication of bacterial DNA selectively, completely, and reversibly, and has no apparent effects on the metabolism of other cellular macromolecules thus far examined. The mechanism of its action has been investigated in uninfected and phage-infected Bacillus subtilis, and the compound appears to be specific for a host function. In cells infected with virulent phage the synthesis of phage DNA proceeds normally, while residual host DNA synthesis is completely blocked. The drug-sensitive host site retains its sensitivity even after partial disruption of the cell by lysozyme treatment.


Abnormally high plasma catecholamines at rest and during exercise in ketotic juvenile diabetics.


Renal excretion of 51Cr-EDTA studied with stop flow technique.


Influence exerted on the growth of heat-injured faecal streptococci by the addition of blood or milk to sodium azide media.


Studies on strain 84 of Clostridium botulinum.


Problems and pitfalls in blood grouping tests for disputed parentage. 3. Chances of proving non-paternity by blood grouping tests when the putative father is dead.


Histochemical study of protein in epithelial tissue of the digestive tract of Pimelodus maculatus Lacépède, 1803.


Analysis of the postnatal development of the rat ovary by histochemical methods.


An attempt of applying laser rays for the detection and analysis of chemical elements in tissue slices.


Comparative biochemical and histochemical studies on the activity of gamma-glutamyl transpeptidase in the organs of fetuses, newborns and adult rats.


Biochemical and histochemical investigations of the gamma glutamyl transpeptidase in embryonal and adult organs of man.


Distribution of the circulation in the normal and asphyxiated fetal primate.


The effect of light on growth and development of two nitrogen fixing blue-green algae.


Studies on live mumps virus vaccine. 3. Evaluation of newly developed live mumps virus vaccine.


Improvement of the method of plaque formation of influenza virus and its application to neutralization tests.


Diagnostic possibilities of lymphography.


On the roentgenological diagnostics of the poststenotic aneurysms of the pulmonary artery.


Complex treatment of the carcinoma of the thyroid gland.


Cases with renal glycosuria in Ethiopia.


Demonstration of antigens related to colonic cancer in the human digestive system.


Azo-dye binding to proteins and detoxication of p. dimethylaminoazobenzene in mouse and hamster liver.


Ampicillin treatment of listeriosis.

Ampicillin treatment of listeriosis.


A soluble antigen of infectious bronchitis virus.


Contamination of umbilical catheters in the newborn infant.


Characterization of precipitating antibodies to ruminant serum and milk proteins in humans with selective IgA deficiency.


Reactions with hemagglutinin of psittacosis agent in gel diffusion.


Brain vulnerability to postnatal protein calorie deficiency in infant rhesus monkeys.


Immunoglobulins in respiratory secretions obtained from the canine tracheal pouch.


Persistence and spread of influenza virus (A2-Hong Kong) in normal and poly I.C. treated baboons (Papio cynocephalus).


Species differences in the metabolism of diphenhydramine (Benadryl).


The inhibition of intestinal absorption of phenylalanine in the rhesus monkey.


Biological properties of a syncytia-forming agent isolated from domestic cats (feline syncytia-forming virus).


Radiation increases the solubility of L cell DNA in a detergent-based lysing system.


Isolation and characterization of the digestive proteinases in the earthworm Lumbricus terrestris Linnaeus.


Hexose-ATP phosphotransferases: comparative aspects.


A nonmineralized concretion in the urogenital tract of the male rat.


Editorial. History and dentistry.

Editorial. History and dentistry.


Dental review committees and consumer participation.


Amyotropic lateral sclerosis and trauma.


Presentation: the 1970 Horace Hayden Award.


It can happen here.

It can happen here.


Presentation: to the Connecticut State Dental Association Central Office. An oil portrait of Pierre Fauchard.


Oral Medicine Seminar. 22--Clinical Oral Pathology of Interest to G.P.


University of Connecticut School of Dental Medicine.


Review. Pulp therapy for the primary dentition. I. The treatment of teeth with vital pulps.


Submental lymphadenectomy.

Submental lymphadenectomy.


The president's perspectives.

The president's perspectives.


Reflections on some contemporary problems in nutrition.


Repair of mandibuladefect using cellulose acetate filter, Kirschner wire and autogenous marrow.


Oral medicine seminar No. 23--the TMJ and related joints.


Nursing in a long-term care facility.

Nursing in a long-term care facility.


Normal cutaneous sensitivity to endotoxin in azotemia.


Pathophysiology and surgical implications of hyperosmolar non-ketotic diabetic coma.


Interstitial and hemorrhagic cystitis: viral, bacterial and fungal studies.


Radiation therapy for the embryonal carcinoma of testis in childhood.


Treatment of hypercalcemias.

Treatment of hypercalcemias.


Towards independence.

Towards independence.


Biochemistry of the central nervous system. Some observations on mass transport.


Azapeptides, a novel class of peptide analogs.


Urological aspects of endemic bladder stones in children.


Collie eye anomaly.

Collie eye anomaly.


Effects of psilocybin, dimethyltryptamine and various lysergic acid derivatives on photically-induced epilepsy in the baboon (Papio papio).


The release of prostaglandin E2 from the bovine iris.


Effects of coronary blood flow and perfusion pressure on left ventricular contractility in dogs.


L-1,4-cyclohexadiene-1-alanine, an antagonist of phenylalanine from Streptomyces.


Antibiotic 6640. 3. Biological studies with antibiotic 6640, a new broad-spectrum aminoglycoside antibiotic.


Isolation and partial characterization of amphibian tyrosine oxidase polysomes.


Sporulation in Bacillus subtilis. Theoretical and experimental studies in continuous culture systems.


Liver esterases of rhesus monkey: inhibition and activation by selected pesticides.


Large Babesia species transmitted to splenectomized calves by field collections of British ticks (Haemaphysalis punctata).


Low molecular weight serum immunoglobulin M in experimental trypanosomiasis.


Neuroanatomy.

Neuroanatomy.


General neurophysiology. (Bioelectric aspects).


Electroencephalography.

Electroencephalography.


Measurement of genetic heterogeneity by means of enzyme polymorphisms, in wild populations of the mouse.


Anticholinergic medication in open-angle glaucoma. Long-term tests.


Anterior chamber paracentesis. Diagnostic value in postoperative endophthalmitis.


Channels of aqueous outflow and related blood vessels. 3. Episcleral arteriovenous anastomoses in the rhesus monkey eye (Macaca mulatta).


The chemotherapy of herpes iritis with adenine arabinoside and cytarabine.


Retinal burn thresholds for the helium-neon laser in the rhesus monkey.


Sulfolipid I of Mycobacterium tuberculosis, strain H37RV. Nature of the acyl substituents.


Long-stay hospitals.

Long-stay hospitals.


Immunochemical differences in deoxyribonucleoproteins from ormal and malignant canine tissues.


The total protein and immunoglobulin profile of equine colostrum and milk.


Growth of Arthrobacter citreus in a chemically defined medium and its requirement for chelating agents.


Structure and composition of resistant layers in bacterial spore coats.


Model of the bacterial growth cycle: statistical dynamics of a system with asymptotic orbital stability.


Instrument for sampling and measuring bile flow.


Surgical nutrition.

Surgical nutrition.


The metabolic production and disposal of acid and alkali.


Demonstration of five serological groups of streptococcal strains resembling Streptococcus mutans.


Urea-extractable antigens in normal, benign, and neoplastic mouse epidermis.


The pH-dependent liberation of phosphate from human dental enamel and dentine by ammonium sulphate.


Nitrogenase activity in the blue-green alga Plectonema boryanum strain 594.


Camptothecin inhibits macromolecular synthesis in mammalian cells but not in isolated mitochondria of E. coli.


Variation in the primary structure of carbonic anhydrase B in man, great apes, and old world monkeys.


On kinetic treatments of enzyme-antienzyme reactions.


Response of argR- spheroplasts of Escherichia coli to extracted arginine repressor.


A particular conformational change of Bacillus cereus penicillinase under the action of a new penicillin analogue pyrazocillin.