Equilibration of atmospheric carbon dioxide with sea water: possible enzymatic control of the rate. Surface and subsurface ocean water differ in exchange characteristics with atmospheric carbon dioxide. The possibility of control by an enzyme like carbonic anhydrase has been experimentally explored.


Studies on the iodine metabolism of the thyroid gland as revealed by electron microscopic autoradiography of 125I.


Circadian rhythms in epidermal mitotic activity. Diurnal variations of the mitotic index, the mitotic rate and the mitotic duration.


Plasma clearance of heated serum bound Co57-vitamin B12.


Adverse effects of steroids in acute myeloblastic and monoblastic leukemia.


Two families with different expression of homozygous beta-thalassaemia.


Haemoglobin D in a Greek family.

Haemoglobin D in a Greek family.


Loss of acetylcholinesterase activity in human erythrocytes treated with cephalothin.


Juvenile xanthogranuloma of the ciliary body in an adult.


Experimental air embolism. Studies with fluorescein cinematography.


Effect of contact lenses on Pseudomonas ulcers in rabbit corneas.


Experimental alpha-chymotrypsin glaucoma.


Experimental cerebral concussion in the rhesus monkey.


The reactions of antibodies with hemeprotein antgens. The measurement of reaction kinetics and stoichiometry by fluorescence quenching.


Purification and characterization of cysteine synthetase, a bifunctional protein complex, from Salmonella typhimurium.


An evaluation of the pathways of metabolism of glucose, gluconate and 2-oxogluconate by Pseudomonas aeruginosa by measurement of molar growth yields.


Immunochemical studies of 22S protein from isolated mitotic apparatus. Evidence is presented that the "22S protein" of mitotic apparatus isolated from sea urchin eggs is not microtubule protein. An antibody preparation active against 22S protein is described, and immunochemical studies of the distribution of 22S protein in various cellular fractions and among morphological features of mitotic apparatus are reported. The protein is ubiquitous in the metaphase egg fractions that were tested but is not found in sperm flagella. It is immunologically distinct from proposed microtubule protein isolated from mitotic apparatus by the method of Sakai, and from proposed microtubule protein obtained after extraction with mild acid. It exists in nontubule material of isolated mitotic apparatus but is not detectable in microtubules.


Lysogenic mycobacteria: phage variations and changes in host cells.


Genetic analysis of ColB factors and the identification of composite circular molecules of R-factors.


Characterization of human cutaneous lipophilic diphtheroids.


The incidence of bacteriocinogeny in some Bacillus species.


Precipitins in mice immunized with attenuated strains of malaria parasites.


The influence of environment on the synthesis of magnesium-binding sites in Bacillus subtilis var. niger cell walls.


Loss of sensitivity to EDTA by Pseudomonas aeruginosa grown under conditions of Mg limitation.


Disinfectant effect of chloroperacetic acids.


Ten-year study of Neisseria gonorrhoeae sensitivity to antibiotics in Czechoslovakia.


Immune globulin prophylaxis of erythroblastosis fetalis.


Carbonic anhydrase in marine algae.

Carbonic anhydrase in marine algae. An electrometric system for determination of carbonic anhydrase activity was constructed. Enzyme activity was assayed in homogenates of marine macroscopic Chlorophyta, Rhodophyta, and Phaeophyta. Plants surveyed included Ulva expansa (Setchell) Setchell and Gardner, Codium fragile (Suringar) Hariot, Enteromorpha sp., Chaetomorpha torta (Farlow) McClatchie (Chlorophyta); Laurencia papillosa (Greville). Plocamium coccineum var. pacificum (Kylin) Dawson, Pterocladia capillacea (Gmelin) Bornet and Thuret. Gigartina armata J. Agardh (Rhodophyta); Eisenia arborea Areschoug and Macrocystis pyrifera (Linnaeus) C. A. Agardh (Phaeophyta). Activity was present in all algae; in the Phaeophyta this could be demonstrated only after dialysis. p-Chloromercuriphenylsulfonic acid (10(-4)m) decreased activity in 1 species, Plocamium; this inhibition could be almost completely overcome with the addition of 10(-3)m dithiothreitol. In 2 green and 2 red algae assayed for sensitivity to acetazolamide (Diamox), inhibition was complete at 10(-4)m concentration of inhibitor. Dithiothreitol at a concentration of 10(-3)m did not enhance activity in any of the homogenates, and was not necessary for enzyme expression.


Trends in radiotherapy.

Trends in radiotherapy.


Neisseria Meningitidis infections in Richmond, September 1966 to August 1968.


Behavior of belladonna mottle virus in serological tests in the presence of organic mercury compounds.


Development of measles virus in vitro.

Development of measles virus in vitro.


Transitional electroencephalographic manifestations between ictal generalized spike-and-wave pattern and generalized repetitive fast discharge of the grand mal type.


Serological and bacteriological investigation of Mycobacterium ranae (fortuitum). Twenty strains of a species of fast growing Mycobacterium, some named M. fortuitum and others said to be similar to the strain of M. peregrinum, in the possession of the National Collection of Type Cultures, were examined serologically and bacteriologically. They were all found to be of the same species, but they were divisible into four serotypes (I to IV), on a basis of three variable antigens, which do not exactly correspond to the subspecies described in previous reports. The strains varied in the number of years that they had been kept in culture and were of widely divergent origin. With the exception of serotype II strains, which all came from sputa from Welsh miners, the serotypes were independent of years in culture or site of origin. Based on bacteriological and serological identity and chronological priority, we consider that M. ranae (Kuster) Bergey et al. should be reinstated in place of M. fortuitum Cruz. A modified scheme for identification of the species is proposed, which would not exclude any of the four serotypes.


Heterogeneity of the antibody response to thyroglobulin: effects of 6-mercaptopurine.


Immunological relationships among some species of extremely halophilic bacteria. Rabbits were immunized with four strains of halobacteria, Halobacterium halobium NRL, H. halobium R-1, H. salinarium NRL-9, and H. cutirubrum NRL-10, that had been fixed with formaldehyde. The antisera obtained detected the presence of an antigen common to the Halobacterium genus and, after absorption, detected three distinct antigenic groups within the Halobacterium genus. A fourth group was agglutinated only by unabsorbed sera.


Plaque assay for Rickettsia rickettsii.

Plaque assay for Rickettsia rickettsii. A plaque technique for the assay of Rickettsia rickettsii is described. The method employs primary chick or green monkey kidney monolayer cell cultures with either an agarose or special Noble agar overlay. Plaques were counted in 6 days and resultant titers correlated well with ld(50) end points obtained by a standard assay in embryonated eggs. Identification of the plaque-forming organisms was accomplished by direct observation of rickettsiae-like bodies in the monolayer lesions, inhibition of plaques by antibiotics, sensitivity of plaques to specific immune serum, and failure to cultivate other microorganisms from the infected cells. Versatility of the test was demonstrated by assaying samples of rickettsiae from several different sources commonly used in our laboratory. These included infected yolk sacs, various cell cultures, and infected guinea pig tissue. Sufficient numbers of viable rickettsiae were present in the cells of a single lesion to permit direct recovery.


Effect of formaldehyde on the immunochemical and biological activity of staphylococcal enterotoxin B. Staphylococcal enterotoxin B was treated with 0.15 or 0.3% formaldehyde at 37 C and pH 7.3. As early as 8 hr after addition of HCHO, the toxin was unable to precipitate as much antibody from antiserum as untreated toxin; greater amounts of treated toxin were required to reach equivalence and to inhibit precipitation. With increasing time, its reactivity decreased so that at 32 weeks it could precipitate only 20% as much antibody as untreated toxin. When other factors were kept constant, the degree of inactivation increased as the HCHO concentration increased from 0.6 to 1.4%. Treated toxin gave a "reaction of partial identity" by the Ouchterlony double-immunodiffusion technique. The emetic effect of the toxin for the monkey remained essentially unchanged for about 48 hr of treatment, but the amount required to cause death by the intravenous route increased from 25 to over 1,000 mug/kg of body weight. HCHO destroyed the pyrogenic effect for rabbits. The treated toxin remained immunogenic for rabbits even after 4 to 5 weeks of exposure to 0.3% HCHO at 37 C.


Effect of formaldehyde on the immunogenicity of staphylococcal enterotoxin B for Macaca mulatta. Monkeys were immunized with enterotoxin and enterotoxoid by intracutaneous injection or by feeding. Identical schedules were used to compare the effectiveness of the two antigens and the two routes. Enterotoxin administered intracutaneously was the most effective antigen, whereas oral administration of enterotoxoid was least effective. Intracutaneous injection of toxoid and oral feeding of toxin were intermediate and not too dissimilar in effectiveness. Antibody titers and protection persisted for at least 1 year at a relatively high level. Monkeys that had preimmunization hemagglutinins showed an anamnestic response after immunization. The development of protection and the appearance of antibodies subsequent to feeding toxin or toxoid suggest that ingestion of food contaminated by staphylococci or their metabolites may be one cause for the appearance of antitoxin in the serum of supposedly unexposed animals and man.


Immunity to cholera: relation of fraction II of type 2 cholera toxin to vibriocidal antibody. The nontoxic protein component in supernatant fluids of young cultures of the cholera vibrio in peptone dialysate broth contains an antigen identical in specificity to vibrio lipopolysaccharide. This material was heterogeneous after elution from diethylaminoethyl A50 Sephadex, and it contained at least five additional minor antigens. Identity was demonstrated by immunodiffusion methods, by the induction of specific vibriocidal antibody formation, and by specific interference in the vibriocidal reaction. The minor antigens appeared to be unrelated to the vibriocidal reaction. The major antigen was more highly immunogenic than lipopolysaccharide, giving higher and longer-persisting antibody titers in the rabbit, but lipopolysaccharide was the more effective interfering antigen per unit weight in the vibriocidal reaction. The nontoxicity and high immunogenic potency of the protein antigen suggest that it may be useful as an immunizing agent for the production of the antibacterial component of an effective immunity.


Antigenic heterology between flagellin and flagella of Bacillus subtilis. The antigenic properties of Bacillus subtilis flagella and periodate-treated flagellin were compared by using immobilization-inhibition and diethylaminoethyl cellulose binding assay. The results suggest that there are both unique and cross-reactive antibodies elicited by B. subtilis flagella and flagellin.


Immunochemistry of the cell walls of Listeria monocytogenes. The antigenic specificity of Listeria monocytogenes types I, II, III, IVa, and IVb was studied by immunochemical techniques. Immunologically active carbohydrates of the various types were extracted from cell walls and were chemically analyzed. Types I and II contained predominantly glucosamine and rhamnose; type III, galactose, rhamnose, and glucosamine; and types IVa and IVb, glucose and galactose. Quantitative precipitin inhibition tests with purified monosaccharides indicated that the major antigenic determinant of types I and II is rhamnose. Precipitin reactions could not be detected with type III carbohydrate and homologous or heterologous antisera. The major determinants of types IVa and IVb were found to be galactose and glucose, respectively. As much as 87% inhibition of the quantitative precipitin test for types I and II was obtained with rhamnose, 72% for type IVa with galactose, and 72% for type IVb with glucose. The immunochemical basis for the antigenic specificity of L. monocytogenes types I, II, IVa, and IVb was further confirmed by using agar gel diffusion. Cross-reactions among the various type-specific carbohydrates and heterologous antisera were also studied. Type II carbohydrate was found to contain galactose and react with type IVa antisera. This reaction could be blocked by galactose. Type I carbohydrate did not contain galactose nor did it react with antiserum prepared from type IVa cells. Therefore, the somatic antigens of type I and type II L. monocytogenes, previously thought to be identical, appeared to differ. The dominant immuno-specific group in the cross-reaction between type IVb carbohydrate and type IVa antisera was found to be galactose. Type IVa absorbed antisera did not produce a significant cross-reaction with type IVb carbohydrate. The results obtained from this investigation indicate a lesser degree of antigenic relationship between type IVa and type IVb L. monocytogenes than was previously believed to exist.


Laboratory characteristics of paratyphoid B vaccines tested in controlled field trials. The immunization of human beings with paratyphoid B vaccines, in doses producing a marked protective effect in field trials, resulted in H-agglutinin titers that were significantly higher than those produced by ineffective doses. In immunization of rabbits and white mice, the same difference in doses had a significant effect on the ability of vaccines to stimulate the formation of H and O antibodies. A parallel was noted between the effectiveness of vaccine for human beings and the activity of sera of the corresponding group of people in the passive mouse-protection test, with a correlation between such activity of sera and the 19S O-hemagglutinin titers. The data obtained suggested as promising the determination of agglutinin dynamics in the sera of inoculated human beings and animals to evaluate the antigenic activity of paratyphoid B vaccines in the laboratory. The passive mouse-protection test with human sera holds promise for laboratory evaluation of the effectiveness of paratyphoid B vaccines. At the same time, none of the variation of the active mouse-protection test employed permitted a significant difference to be detected between the vaccines effective and ineffective for man. Comparison of data on the frequency and degree of febrile vaccine reactions in man with results of laboratory evaluation of vaccine toxicity showed that, provided a reference vaccine with known pyrogenic capacity for man was employed, the study of stress effect of vaccines on guinea-pigs (determination of 17-oxycorticosteroid content) may permit prediction of the reactive power of the vaccine. No correlation was found between the pyrogenic capacity of vaccines for man and their toxicity for mice.


Mixotrophic growth of Hydrogenomonas eutropha. Mixotrophic growth conditions were established by the addition of lactate to cultures of Hydrogenomonas eutropha growing autotrophically in a gaseous environment of H(2), O(2), and CO(2) (6:2:1). The specific growth rate of mixotrophic cultures was double that of the autotrophic cultures, and lactate disappearance paralleled growth. Growth yields in mixotrophic cultures were significantly greater than those in heterotrophic cultures for equal quantities of lactate consumed. The magnitude of the increase in yield was directly proportional to the absolute growth rate at the time of lactate addition to the starting autotrophic culture and to the time under mixotrophic conditions. The specific activities of hydrogenase and ribulose diphosphate carboxylase decreased during mixotrophic growth; the total activities increased somewhat. The results suggested that the complete autotrophic and heterotrophic physiologies functioned simultaneously under mixotrophic contions.


Capacity of aspartic acid to increase the bacterial count on suspensions of Escherichia coli after freezing. The addition of 2% Trypticase to a minimal salts-glucose plating medium increased the bacterial count of frozen and thawed suspensions of Escherichia coli 451B cells, even when precautions were taken to remove toxic trace elements from the plating diluent. Hydrolysis of the Trypticase with HCl or H(2)SO(4) reduced its count-increasing activity. Treatment of the H(2)SO(4) hydrolysate with a cation-exchange resin greatly improved its capacity to replace Trypticase. Addition of a mixture of amino acids approximating the composition of casein also increased the plate count when added at a level equivalent to 0.1% casein, but at 2% it depressed the count. Tests of amino acids in the mixture revealed that aspartic acid could replace Trypticase completely as a supplement to the basal medium. When added at a level of 2.5 mm, aspartic acid doubled and occasionally tripled the plate count of a suspension of frozen and thawed cells. Glutamic acid, alanine, and to a lesser extent certain other amino acids also showed a capacity to increase the count. Cysteine was without significant effect. Serine and other amino acids depressed the count. None of the amino acids or other supplements affected the count of suspensions of cells that had not been frozen. The effect of adding aspartic acid, cysteine, or Trypticase to the basal medium on the bacterial count of suspensions of various strains of E. coli, Aerobacter aerogenes, Serratia marcescens, and two species of Pseudomonas after freezing was examined. The response to the supplements was unique for each organism.


Regulation of synthesis of glutamine synthase in Bacillus subtilis. A study of the regulation of the synthesis of the enzyme glutamine synthase in Bacillus subtilis was initiated. An assay, based on the measurement of glutamo-hydroxamate, was used to characterize the enzyme in crude preparations and in toluene-treated cells. Determinations were made of the Michaelis constants for adenosine triphosphate, hydroxylamine, and glutamate (9 x 10(-3), 4 x 10(-3), and 2.2 x 10(-2)m, respectively), the pH optimum (7.6 to 7.7), and the stability. The differential rate of synthesis was determined under various growth conditions. The enzyme was found to be relatively insensitive to regulation. Partial repression was caused by glutamine, arginine, asparagine, and glutamate, or by carbon limitation in a chemostat. Derepression was caused by exhaustion of externally added amino acids or by nitrogen limitation in a chemostat.


Effect of enterotoxin B on human volunteers. A dosage of 20 to 25 mug of pure enterotoxin B can produce clinical manifestations of enterotoxemia in man, who seems to be more sensitive than monkey.


New immunotype schema for Pseudomonas aeruginosa based on protective antigens. The development of an antigen schema for Pseudomonas aeruginosa based on challenge protection in mice as distinguished from serological tests in vitro was described. For 342 cultures, seven groups of cross-protective homogeneity were defined.


A machine operating on punched-tape instructions for teaching visual discriminations to monkeys.


Actinomycin D sensitivity of bacteria with simple and complex cell surfaces. The sensitivity of different bacteria to actinimycin D was not related to their cell surface morphology. Bacteria with simple and complex surfaces were both extremely susceptible.


Control of body temperature in the unanaesthetized monkey by cholinergic and aminergic systems in the hypothalamus. 1. In the unanaesthetized rhesus monkey, 5-hydroxytryptamine (5-HT), catecholamines, acetylcholine or carbachol were micro-injected in a volume of 1.0 mul. or less through chronically implanted cannulae at eighty-six sites in the hypothalamus.2. 5-HT in doses of 2-10 mug caused a long-lasting elevation in temperature which was dose-dependent. An anatomical ;mapping' of the hypothalamus revealed that the hyperthermic action of 5-HT was localized to the anterior, pre-optic area directly ventral to the anterior commissure.3. Noradrenaline in doses of 1-12 mug produced a dose-dependent fall in temperature of short duration. An anatomical ;mapping' showed that the hypothermic action of this and other catecholamines was again localized to the anterior, pre-optic region.4. Acetylcholine, alone or in a mixture with eserine, or carbachol caused a dose-dependent hyperthermia which was characterized by an intense rise of short duration and vigorous shivering. A ;mapping' of this response revealed a diffuse patterning of sites throughout the hypothalamus which were sensitive to the application of acetylcholine and carbachol. However, in one circumscribed region at the junction between the posterior hypothalamus and mesencephalon, the two cholinomimetic substances caused a marked fall in temperature.5. We conclude that 5-HT activates a cholinergic heat production pathway which projects from the anterior to posterior hypothalamus. Noradrenaline, on the other hand, blocks the hyperthermic action of 5-HT rather than activates the heat loss system. A chemically mediated heat loss pathway apparently does not exist in the hypothalamus.


Quantitative investigations of the rapid swelling reaction of blood platelets.


The several paths to quality.

The several paths to quality.


Cholangiography vs extraductal palpation.


Experimental hemorrhagic shock: 1. Pharmacologic effect of phenoxybenzamine and epinephrine on the small vessels and microcirculation.


Why do doctors stay in the army?

Why do doctors stay in the army?


Falciparum malaria in Vietnam: clinical manifestations and nursing care requirements.


Renal cell carcinoma metastatic to the pancreas thirteen years following nephrectomy.


Physiological costs of body armor.

Physiological costs of body armor.


The medical support of guerrilla forces.


Microbiological aspects of food irradiation.


The effects of the riot control agent CS on visual acuity.


The origin of cytoplasmic inclusions from the nuclear envelope of mammalian oocytes.


Experimental scurvy in man.

Experimental scurvy in man.


Antigenic and deoxyribonucleic acid base composition of a Mycoplasma and associated diphtheroids.


Serological analysis of eleven strains of Rhizobium japonicum.


Autoradiographic and immunofluorescent studies of cytological changes induced in Bacillus cereus by chloramphenicol.


Experimental autoimmune gastritis in the Rhesus monkey.


Simplified method of filling the wells accurately in gel immunodiffusion plates.


A latex agglutination test for the measurement of antibodies to group-specific streptococcal polysaccharides.


Examination by electron microscopy of the San Carlos viruses isolated from children with infectious hepatitis. The results of a study by electron microscopy of the cytopathic effects produced by the San Carlos viruses on cultured human embryo hepatocytes are reported. Examination of liver cells infected with San Carlos virus 6 revealed single virions and crystalline arrays with the hexagonal virion contour measuring an average of 65 to 70 mmu. However, associated with this predominant virion a smaller virus particle with a dimension of 30 to 40 mmu was also found. The nature of this second virion type is not yet clear but its diameter is much larger than the size so far described for the adeno-associated viruses. The size of the smaller virion is probably also too large for it to be a serious candidate for the hepatitis virus but the possible role of these agents in the aetiology of infectious hepatitis, at least in some patients, merits further study.


Neuropsychiatric syndromes of liver disease.


Diabetes mellitus and blood groups.

Diabetes mellitus and blood groups.


Mass screening of school children for heart disease.


Camp Concern.

Camp Concern.


Use of case survey technique to detect origin of Blastomyces infections.


A critical review and evaluation of smoking control methods.


Attitudes of health department clients toward an error in immunization.


Maternal knowledge of nutritional anemia.


Identification of transmission foci of hydatid disease in California.


School physicians' views of relations with medical and nonmedical agencies.


Survival of micro-organisms in aerosols produced in cleaning and disinfecting.


Cervical screening with the Davis pipet on a door-to-door basis.


Susceptibility to epidemic rebella in a college population.


A foodborne outbreak of shigellosis on an Indian reservation.


The chemistry of proteases.

The chemistry of proteases.


Pancreatitis--experimental and clinical: an evaluation of trypsin-kallikrein inhibitor.


Purification and properties of an exo-beta-D-1,3-glucanase from sea urchin eggs.


Prevention of primary Rh immunization: first report of the Western Canadian trial, 1966-1968.


Purification and properties of the 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (phenylalanine sensitive) of Escherichia coli K12. II. Inhibition of activity of the enzyme with phenylalanine and functional group-specific reagents.


Microdiverticula: a possible cause of granulomatous ileocolitis.


Application of electron microscopy to the diagnosis of virus infections.


Interictal focal or lateralized discharges occurring in the electroencephalograms of patients suffering from centrencephalic epilepsy.


Effects of thyrocalcitonin on calcium transport in kidney cells.


Growth hormone secretion by pituitary grafts under the median eminence or renal capsule.


Flotation hydrocarbon technique in isolation of tubercle bacilli from cerebrospinal fluid in tuberculosis meningitis.


Determination of iron in solutions containing iron complexes. A method of estimating the iron content of solutions containing haemoglobin, ferritin, or ferrioxamine is described. Iron is released by treatment with acid permanganate and ascorbic acid before conventional determination by an Auto Analyzer technique.


Serum haptoglobin type in females with genital cancer.


Induction of transitional cell carcinomas of the urinary bladder in monkeys fed 2-naphthylamine.


Comparison of radioisotopic and column chromatographic assay of serum thyroxine.


Research prospects--rubella vaccine.

Research prospects--rubella vaccine.


Localization of 113mIn-chelates compared with 99mTc-sodium pertechnetate in experimental cerebral lesions.


Propranolol in angina pectoris.

Propranolol in angina pectoris.


Gonorrhoea and non-specific urethritis.

Gonorrhoea and non-specific urethritis.


The laboratory diagnosis of gonorrhoea in the female.


On the sympathetic control of ventricular automaticity. The effects of a reflex increase of sympathetic discharge.


The specific trypsin inhibitor from pig pancreas.


Lysozymes: a chapter of molecular biology.


Structure and biosynthesis of the ergot pigments.


Metabolic transformation of antimalarial drugs as a basis for the development of new active substances.


Sterol metabolism of Plasmodium knowlei in vitro.


The pharmacology of 4H-3-methylcarboxyamide-1,3-benzoxazine-2-one (F.I. 6654), a compound acting on the central nervous system.


Human pulp reactions to a glucocorticosteroid-antibiotic compound.


The enthalpy of binding of various transition metal ions to bovine apocarbonic anhydrase.


35C1 nuclear magnetic resonance studies of a zinc metalloenzyme carbonic anhydrase.


Jack bean urease (EC 3.5.1.5). A new purification and reliable rate assay.


Studies of myosin conformation by fluorescent techniques.


Antibiotics and polyribosomes. II. Some effects of lincomycin, spiramycin, and streptogramin A in vivo.


Reversible cardiac failure during angina pectrois: hemodynamic effects of atrial pacing in coronary artery disease.


Fractionation of nucleic acids using polylysine kieselguhr chromatography.


Antigen-antibody reactions in agar. IX. Treatment of antisera and optical densities of precipitin reactions.


Antigens of normal and atherosclerotic intima.


Distribution of poliovirus antibody in serum, nasopharynx and alimentary tract following segmental immunization of lower alimentary tract with poliovaccine.


Immunochemical studies on cobrotoxin.

Immunochemical studies on cobrotoxin.


A simplified microplate cell culture system.


Individual patterns of ram seminal antigens.


Evaluation of a new antituberculous agent. Ethambutol hydrochloride (Myambutol).


The "area centralis" of the owl monkey (Aotes trivirgatus).


The effect of UV light and mitomycin C on the transfection of Bacillus subtilis 168 I.


Histochemical studies on the spinal cord of squirrel monkey (Saimiri sciureus).


The antibody mechanism of ragweed allergy-electrophoretic and chemical studies. V. Large scale serum fractionation by the rivanol method and procedures to enrich the reagin of G-fractions into small volumes.


Preparation of antisera monospecifically reactive with the fourth component of guinea pig complement.


Olfactory discrimination in monkeys with lesions in the amygdala.


Formation of agglutinating and reaginic antibodies in rabbits following oral administration of soluble and particulate antigens.


The effect of fetal and maternal factors on radiological maturation of the fetus.


Early secondary disease in the Rhesus monkey. I. A comparative histopathologic study.


Induction of tolerance for ALG.

Induction of tolerance for ALG.


Changes in morphology of cell cultures after treatment with aflatoxin and ochratoxin.


Prolonged hospitalization of paraplegic patients.


Arterial hypertension in the squirrel monkey during behavioral experiments.


Behavioral induction of arterial hypertension and its reversal.


Advanced human lymphocyte response to coccidioidin in vitro following skin test.


Use of radioactive tracers to determine the protective effect of cavity liners.


Portable ethylene oxide sterilization chamber. A portable ethylene oxide sterilization chamber was designed, constructed, and tested for use in the sterilization of embolectomy catheters. The unit can accommodate catheters up to 40 inches (101.6 cm) in length and can be operated for less than 4 cents per cycle. A constant concentration of 500 mg of ethylene oxide per liter of space and holding periods of 4 and 6 hr at 43 and 22 C, respectively, were adequate when tested with B. subtilis spores. The estimated cost of construction was $165.00. If temperature control is unnecessary, the cost is approximately $80.00.


Production of antibody against conjugated dipicolinic acid (2,6 pyridine dicarboxylic acid). An immunological precipitation system, with rabbit dipicolinyl antibody, was developed for the purpose of evaluating the role of spore dipicolinic acid as a specific haptenic determinant.


Influence of sodium fluoride on healing of experimental fractures in rats, squirrel monkeys, and dogs.


The effect of nitrate explosives on adrenergic nerves.


Carcinoma of the bladder treated by suprapubic radium implants. The value of additional external irradiation.


Bilateral acoustic neurinoma and neurofibromatosis.


Urinary problems of spina bifida.

Urinary problems of spina bifida.


Heterogeneity and globin composition of adult chicken hemoglobin.


Hypotonic duodenography.

Hypotonic duodenography.


A relation between positive phase shift and elastic modulus enhancement of smooth muscle.


Polymeric collagen isolated from the human intestinal submucosa.


Studies of reagins to alpha-gliadin in a patient with wheat hypersensitivity.


Effects of beta-propiolactone (Betaprone) on reaginic (skin-sensitizing) antibodies.


Asthma due to inhalation of moth flies (Psychoda).


Characterization of the ribonucleoprotein and neuraminidase of influenza A viruses by immunodiffusion.


Genetic characteristics of echovirus type 9 strains: association of mouse virulence with other genetic markers.


Pineal response to light: mediation by the accessory optic system in the monkey.


The treponematoses.

The treponematoses.


The prognostic value of maternal RH-antibody inhibition studies and liquor amnii investigations.


An investigation into wound infection and abscess formation in a mine.


Lysosomal hydrolases in experimental amyloidosis.


Synchrony of restricted gene transcription in mammalian sister cells in culture.


A role for carnitine and long chain acylcarnitine in the regulation of lipogenesis.


Levels of a component characteristic of amyloid substance in sera from normal persons and patients with amyloidosis.


Carcinoma of the tongue and floor of the mouth. Results of radical radiotherapy.


Studies on the mechanism of action of thyrocalcitonin: inhibition with acetazolamide.


Chorionic gonadotropin excretion during pregnancy in the marmoset, Callithrix jacchus.


Depression of thyrotropin releasing factor induction of thyrotropin release by throxine in small doses.


Importance of thyroid hormones in bone metabolism and calcium homeostasis.


Transformation of phage-resistance in Bacillus subtilis.


Cardiorespiratory and metabolic responses to liver E. coli and endotoxin in the monkey.


Isolation of amino acid transport-negative mutants of Pseudomonas aeruginosa and cells with repressed transport activity. Methods are described for the isolation of amino acid transport-negative mutants of Pseudomonas aeruginosa and for the preparation of cells with repressed, specific amino acid permeases. P. aeruginosa was resistant to high concentrations of the majority of the 53 amino acid analogues examined and was unaffected by low concentrations of any of them. Cells which had been grown in the presence of sublethal concentrations of the few analogues which were inhibitory were subsequently more resistant to the analogues. These cells were also defective in the transport of the corresponding amino acid, as the analogue caused repression of the synthesis of the specific amino acid permease. The cells with repressed transport activity rapidly regained their normal level of constitutive permease when grown in the absence of the analogue. Higher levels of the permeases were induced when these cells were grown in the presence of the appropriate amino acid. The possible mechanisms for the mode of regulation of amino acid permeases are discussed.


Effect of lysozyme on resting spores of Bacillus megaterium. Resting spores of Bacillus megaterium ATCC 9885 were found to be markedly affected by lysozyme. Exposure to as little as 1.5 mug of lysozyme per ml caused the spores to lose refractility, the darkened spores to shed their coat structures, and the spore central bodies to lyse. The spores of seven other strains of B. megaterium and seven other Bacillus species were not similarly affected by lysozyme. Proteolytic enzymes such as pronase, trypsin, pepsin, and subtilisin did not induce the change. The action of lysozyme differed in certain important respects from that of common "physiological" germinants. Its action was considered to be direct via its enzymatic attack on exposed sites directly accessible in the resting spores of B. megaterium ATCC 9885.


Biochemical studies of bacterial sporulation and germination. XII. A sulfonic acid as a major sulfur compound of Bacillus subtilis spores. A sulfonic acid found to be a major constituent of spores of Bacillus subtilis was provisionally identified as 3-l-sulfolactic acid. This compound was completely absent from vegetative cells during growth, but large amounts accumulated in sporulating cells just before the development of refractile spores. Essentially all of the accumulated sulfolactic acid was eventually incorporated into the nature spore, where it may represent more than 5% of the dry weight of the spore. Germination resulted in the rapid and complete release into the medium of unaltered sulfolactic acid. This compound was not found in spores of Bacillus megaterium, B. cereus, or B. thuringiensis.


Regulated enzymes of aromatic amino acid synthesis: control, isozymic nature, and aggregation in Bacillus subtilis and Bacillus licheniformis. Several regulated enzymes involved in aromatic amino acid synthesis were studied in Bacillus subtilis and B. licheniformis with reference to organization and control mechanisms. B. subtilis has been previously shown (23) to have a single 3-deoxy-d-arabinoheptulosonate 7-phosphate (DAHP) synthetase but to have two isozymic forms of both chorismate mutase and shikimate kinase. Extracts of B. licheniformis chromatographed on diethylaminoethyl (DEAE) cellulose indicated a single DAHP synthetase and two isozymic forms of chorismate mutase, but only a single shikimate kinase activity. The evidence for isozymes has been supported by the inability to find strains mutant in these activities, although strains mutant for the other activities were readily obtained. DAHP synthetase, one of the isozymes of chorismate mutase, and one of the isozymes of shikimate kinase were found in a single complex in B. subtilis. No such complex could be detected in B. licheniformis. DAHP synthetase and shikimate kinase from B. subtilis were feedback-inhibited by chorismate and prephenate. DAHP synthetase from B. licheniformis was also feedback-inhibited by these two intermediates, but shikimate kinase was inhibited only by chorismate. When the cells were grown in limiting tyrosine, the DAHP synthetase, chorismate mutase, and shikimate kinase activities of B. subtilis were derepressed in parallel, but only DAHP synthetase and chorismate mutase were derepressible in B. licheniformis. Implications of the differences as well as the similarities between the control and the pattern of enzyme aggregation in the two related species of bacilli were discussed.


Biochemical studies of bacterial sporulation and germination. XIV. Phospholipids in Bacillus megaterium. The principal phospholipids of Bacillus megaterium throughout the cycle of growth and sporulation were found to be phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, and a hitherto unidentified isomer of glycosaminyl-phosphatidylglycerol. Phosphatidylglycerol predominated during vegetative cell growth and then declined as spores developed, whereas diphosphatidylglycerol became more prominent during spore maturation. The latter phosphatide was relatively inaccessible in the vegetative cell and was more accessible in the spore, as judged by solvent extraction under various conditions.


Anatomical studies of the vascular system of the brain of Macaca cyclopis (Swinhoe).


Reactions of sera and nasal secretions with staphylococcal antigens.


Immunologic studies of cerebrospinal fluid proteins in experimental aseptic meningitis in dogs.


Respiratory responses in subhuman primates with immediate type hypersensitivity.


Inhibition of the long-acting thyroid stimulator (LATS) by soluble thyroid fractions.


Lead in mentally retarded children.

Lead in mentally retarded children.


Problems and pitfalls in blood grouping tests for non-parentage. II. The Rh-Hr blood group system.


Chemoprophylaxis of infection in patients receiving steroids, irradiation and cytotoxic agents.


Topical antibiotic treatment of infected dental pulps of monkeys.


A new series of 2-aminomethyltetrahydroquinoline derivatives displaying schistosomicidal activity in rodents and primates.


Oxidation of carcinogenic azo-dyes. 3. Metabolites of dimethylaminoazobenzene in the bile of rats.


Maternal viral infections and fetal ocular malformation.


Serum fructose levels in male and female baboons following single intravenous infections of fructose.


Pediatric neurology.

Pediatric neurology.


Clinical study of Chagas' disease (trypanosomiasis cruzi).


High-dose radiotherapy for carcinoma of the cervix. Method and results.


Cinecystourethrography in the diagnosis of urethral diverticula of women.


Epikeratoprosthesis. Replacement of superficial cornea by methyl methacrylate.


Intra-amniotic inoculation of rubella virus.


Hemodynamic effect of listerial hemolysin in dogs.


Species variability in hemodynamic response to paired-pulse stimulation.


Influence of initial resistance on magnitude of response to vasomotor stimuli.


Comparison of in vitro and in vivo placental permeability measurements.


Rhesus isoimmunization and therapeutic abortion.


Factors affecting the yield and biological activity of lipid extracts of Listeria monocytogenes.


Simultaneous production of three phenazine pigments by Pseudomonas aeruginosa Mac 436.


A microbiological assay system for naturally occurring folates.


The effect of ethylenediaminetetraacetic acid on actinobolin-resistance in Escherichia coli.


Endotoxin-induced regional circulatory changes in the unanesthetized monkey.


Specific binding radioassay of serum thyroxine.


The glycogen phosphorylase system from the muscle of the blue crab: Callinectes danae.


Study of the soluble lens proteins from different amphibian species.


Visual pigments and the early receptor potential of frog and monkey retina.


Regional LDH-patterns in mammalian retina.


Phenol oxidases and morphogenesis in Podospora anserina.


The metabolism of bacterial nucleic acid and of free components of nucleic acid by the rumen ciliate Entodinium caudatum.


Aflatoxin B1 binding and toxic effects on Bacillus megaterium.


Studies on the cell walls of pseudomonas species resistant to ethylenediaminetetra-acetic acid.


Inhibition of genetic transformation in Bacillus subtilis by phenethyl alcohol.


Temperature-sensitive DNA synthesis in a mutant of Bacillus subtilis.


Meningitis due to lymphocytic choriomeningitis virus endemic in a hamster colony.


Effect of Polysorbate 80 on cell leakage and viability of Pseudomonas aeruginosa exposed to rapid changes of pH, temperature and tonicity.


Tuberculosis in Muruts of Pensiangan in Sabah.


Primary reading epilepsy.

Primary reading epilepsy.


Toxins and mode of envenomation of the common ringed or blue-banded octopus.


A placebo response as a conditional reflex to glyceryl trinitrate.


Antibody formation in persistent tolerant infection with lymphocytic choriomeningitis virus.


Adrenal glands and sexual receptivity in female rhesus monkeys.


Recovery of human mycoplasmas from simian tissues.


Possible role of toxin in the formation of virus-like plaques by Pseudomonas aeruginosa.


Skin-fixing properties of papain digested rheusus antihapten antibody.


Gross and histologic changes in the jaws of the Macaca rhesus monkey following surgery.


Electron microscopic radioautography: identification of origin of synaptic terminals in normal nervous tissue. A method combining intraocular injection of tritiated leucine and electron microscopic radioautography has been used to study the distribution of optic nerve terminals in the monkey lateral geniculate nucleus. Radioactive label is found in only one type of synaptic terminal, which demonstrates that this terminal is of retinal origin and that this approach can be used to study the origin of synaptic terminals in normal tissue.


Studies on the inactivation of thyrotropin-releasing hormone (TRH).


Experimental observations on the antimycobacterial activity of rifampin.


Binding and metabolism of nitroglycerin by rat blood plasma.


Respiratory syncytial virus neutralizing activity in nasal secretions following natural infection.


Effects of antifertility agents on male mice as determined by a serial mating method.


Studies on the thyrotropin-releasing hormone (TRH) activity in peripheral blood.


Alkylation damage and its repair.

Alkylation damage and its repair.


Analysis of DNA fine secondary structure in relation to replication and transcription.


Can defective segregation prevent initiation?


Temperature-sensitive mutants of B. subtilis defective in DNA synthesis.


DNA-membrane complex and nuclear segregation in bacteria.


DNA replication studied by a new method for the isolation of cell membrane-DNA complexes.


Association-dissociation phenomena in glycinin.


Membrane attachment of the chromosome replication origin in Bacillus subtilis.


Immunological studies on the L-glutamic acid decarboxylase from Escherichia coli K-12.


Quantitative studies on the neutralization reaction between African horse-sickness virus and antiserum.


Herpes T virus plaque assay studies.

Herpes T virus plaque assay studies.


Isolation of the hybrid between ribosomal RNA and DNA of Bacillus subtilis.


Herpes T virus variants. Isolation and characterization.


A comparative study of 63 strains of ECHO virus type 30.


Ion transport by heart mitochondria. XIV. The mannitol-impermeable compartment of the mitochondrion and its relation to ion uptake.


Studies of human erythrocyte catalase by fluorescent antibody technic. The distribution of catalase in acatalasia and hypocatalasia.


Gonococci insensitive to penicillin.

Gonococci insensitive to penicillin.


Immunologic studies in pernicious anemia.


Hemodynamic effects of nitroglycerin, propranolol, and their combination in coronary heart disease.


RBE of the M.I.T. medical therapy neutron beam in monolayer cultures of hela cells.


Electrical stimulation of monkeys' prefrontal cortex during delayed-response performance.


The arterial and venous blood supplies to the cerebellum of primates.


Cytopathic effects of Visna Virus in cultured mammalian nervous tissue.


Tadpole antibodies against frog hemoglobin and their effect on development.


Cortical and subcortical secondary epileptogenesis.


Uncus and amygdala lesions: effects on social behavior in the free-ranging rhesus monkey.


Toxicologic and pharmacologic evaluation of phleomycin including special studies on its nephrotoxicity.


Production of kidney tissue cultures from African green monkeys, experimentally infected with the causative agent of Frankfurt-Marburg-syndrome.


Endotoxin shock in the subhuman primate. I. Hemodynamic and biochemical changes.


In vitro effect of iron salts and chelating agents on serum tuberculostasis.


Army heart pump for postsystolic augmentation.


An anti-Brucella principle of peritoneal macrophages.


Restoration of valine acceptor activity by combining oligonucleotide fragments derived from a Bacillus subtilis ribonuclease digest of Escherichia coli valine transfer RNA.


Sensitivity to penicillin of Neisseria gonorrhoeae. Relationship to the results of treatment.


Preferential synthesis of extracellular ribonuclease and some intracellular proteins in actinomycin D-treated Bacillus subtilis.


Inhibition of Neisseria gonorrhoeae by nifuratel.


Further observations on strain sensitivity of Trichomonas vaginalis to metronidazole.


Multiple molecular forms of avian aldolases. I. Crystallization and physical properties of chicken (Gallus domesticus) breast muscle aldolase.


The inactivation of Bacillus subtilis transforming DNA by mutagenic agents.


Application of immunoprecipitation in agar gel for the serological typing of soybean root-nodules.


The protein and lipid composition of arterial elastin in ageing and atherosclerosis.


Regulation of the formation of protease in Bacillus megaterium. 3. Enzyme production under limitation of nitrogen source.


Rat 7S immunoglobulins: characterization of gamma-2- and gamma-1-anti-haptens antibodies.


Abortion of infection by Bacillus subtilis phage PBS-1 caused by actinomycin-D.


Activity of pyramidal tract neurons during postural fixation.


Time structure of discharges in single auditory nerve fibers of the squirrel monkey in response to complex periodic sounds.


Cortical neuronal mechanisms in flutter-vibration studied in unanesthetized monkeys. Neuronal periodicity and frequency discrimination.


Experimental in vivo microcirculatory dynamics in brain trauma.


The national scene.

The national scene.


Esophageal diverticulum.

Esophageal diverticulum.


The toxicology and epidemiology of mycotoxins.


Selection of posterior tooth forms and management of occlusion for the aging and debilitated patient.


The value of a dental hygienist.

The value of a dental hygienist.


A report on dental assistants training.

A report on dental assistants training.


Teaching prosthodontics by sign language.


Bridging the communication barrier.

Bridging the communication barrier.


The muddy sign of exhaustion. Developer gives clue for changing solutions.


Dental education by TV most effective presentation of clinical procedures.


Dentist with his heart in music. Variation on a career theme by Wilfred Josephs.


Fluoridated salt instead of water.

Fluoridated salt instead of water.


The complete clinic in a caravan.

The complete clinic in a caravan.


When a patient has diabetes avoid intravenous or inhalation anesthesia.


Better wound healing.

Better wound healing.


Anterior gingivectomy reassessed presenting the case for conservative curettage.


A programme of research to improve practices.


The dental surgeon as a diagnostician. Under every specialist, a team of auxiliaries to do routine amalgam restorations.


Sentenced to a hammering.

Sentenced to a hammering.


Oral cancer: the need for biopsies. Risk by surgical interference has been over-emphasised.


Inevitability of group practice.

Inevitability of group practice.


Contour mapping the face. Aerial survey techniques aid orthodontics.


Ideal sedative for the nervous.

Ideal sedative for the nervous.


A pioneer in forensic dentistry; John Furness has helped to convict six murderers.


A survey of hand instrumentation in restorative and surgical dentistry.


Surgery design for methohexitone: less OPERATING STRESS AND AN UNHURRIED PROCEDURE.


Transfer of iodide into amniotic fluid by the normal and the nephrectomized subhuman primate fetus.


The structure of the purine precursor in riboflavin biosynthesis.


A new type of corrin synthesis.

A new type of corrin synthesis.


Synthesis of testosterones from estrogens.


Multiple atresia by ileal and colonic septa: case report.


The treatment of renal tuberculosis.

The treatment of renal tuberculosis.


Studies on drug organotropism and drug-metabolizing activity in the fetus and the newborn.


Hyperosmolar states in the chronically ill.


1-Phenycycloalkylamine derivatives. II. Synthesis and pharmacological activity.


Repository drugs. V. 4',4'''-[p-phenylenebis(methylidyneimino-p-phenylenesulfonyl)]bisacetanilide (PSBA) and related 4',4'''-[bis(imino-p-phenylenesulfonyl)]bisanilides, a novel class of long-acting antimalarial and antileprotic agents.


Synthesis of some new N-o-tolyl-N'-2-(substituted) benzothiazolylguanidines.


Distribution of ABO isohaemagglutinins among fertile and infertile women.


Immunochemical studies on human seminal plasma. II. The major antigens and their fractionation.


Current treatment for renal tuberculosis.


Profound selective cooling and ischemia of primate brain without pump or oxygenator.


Review of bacteriology and preclinical studies and clinical pharmacology of gentamicin sulfate.


The proportionality between bacillary dilutions and the yield of colonies on solid media.


The protein components of Mycobacterium phlei: fractionation procedures.


Differentiation of Mycobacterium tuberculosis and Mycobacterium bovis.


Biochemical and cultural characters of serologically grouped Pseudomonas aeruginosa strains.


Regulation of alkaline phosphatase synthesis in auxotrophic mutants of Bacillus subtilis.


Association between the chromosome and the cytoplasmic membrane in Bacillus subtilis.


Evidence for the bioactivation of slaframine.


Influence of fatty acid composition of infant formulas on development of arteriosclerosis.


Predominant coronary and cerebral atherosclerosis in captive nonhuman primates.


Immunologic studies of the prostate. A review.


Germination of single bacterial spores.

Germination of single bacterial spores. Changes in refractility and optical density occurring in individual spores of Bacillus cereus T and B. megaterium QM B1551 during germination were investigated by use of a Zeiss microscope photometer. The curves revealed that the germination process in single spores had two distinct phases; an initial rapid phase was followed by a second slower phase. Under the experimental condition employed, the first phase of germination of B. cereus spores lasted for approximately 75 +/- 15 sec, whereas the second phase lasted for 3 to 4.5 min. In B. megaterium spores, the first phase was observed to last for approximately 2 min and the second phase for more than 7 min. The duration of the second phase was dependent on conditions employed for germination. The kinetics of the first phase were strikingly similar under all conditions of physiological germination. Time-lapse phase-contrast microscopy of germinating spores also revealed the biphasic nature of germination. It was postulated that the first phase represents changes induced by an initial partial hydration of the spore and release into the medium of dipicolinic acid, whereas the second phase reflects degradation of the cortex and hydration of the core.


Serological comparison of ten glycolytic Mycoplasma species. Seventeen strains of mycoplasmata representing 11 named species were compared serologically by three parameters: growth inhibition on agar, double immunodiffusion, and complement fixation. In growth-inhibition studies, a strain labeled Mycoplasma histotropicum was found related to and perhaps best classified as M. pulmonis, a relationship confirmed by double immuno-diffusion studies. A comparison of the remaining 10 species demonstrated that two pairs of species could be shown to be closely related by complement fixation and double immunodiffusion but not by growth inhibition; these were: M. granularum-M. laidlawii and M. felis-M. canis. M. pneumoniae and M. gallisepticum were the most serologically unique organisms in this study, showing very few cross-reactions with each other or other species. Overall, taxonomic groupings obtained by comparative serology appeared to correlate with the groupings obtained when the guanine plus cytosine contents of the deoxyribonucleic acid of mycoplasmata were employed as classification criteria. The group of organisms having a guanine plus cytosine content of 23 to 28% (M. canis, M. fermentans, M. hyorhinis, M. neurolyticum, and M. pulmonis) appeared to be generally serologically related. Thus the remarkable heterogeneity observed in the base composition of the deoxyribonucleic acid of order Mycoplasmatales is also reflected and apparently paralleled by a corresponding serological heterogeneity.


Chromosomal location of genes regulating resistance to bacteriophage in Bacillus subtilis. Many of the viruses which infect Bacillus subtilis require glucosylated polyglycerol teichoic acid for adsorption. These mutants can be divided into three classes on the basis of enzymatic defects and growth on galactose-minimal medium. Transduction with phage PBS1 reveals that two of these, gtaA and gtaB, are linked to hisA1, whereas the gtaC locus is linked to argC. Analysis by deoxyribonucleic acid-mediated transformation indicates that these loci exist in a cluster between the hisA1 and argC4 loci. Anomalies in mapping in the group II region of the chromosome exist. The basis of these anomalies is discussed.


Significance of 12S toxin of Clostridium botulinum type E. The pathogenesis of type E botulism is discussed as an aspect of the physicochemical and biological properties of 12S toxins (prototoxin and trypsin-activated 12S toxin) and the Ealpha and Ebeta components of each 12S toxin. A molecular weight of 350,000 was determined for each 12S toxin and 150,000 for Ealpha and Ebeta. Owing to the structure comprising the subunits Ealpha and Ebeta, 12S toxins are much more stable than Ealpha at low pH values and high temperatures. Such was also the case with type A 19S toxin and its alpha component. The Ealpha component alone accounts for the total toxicity of type E toxin. The toxic substance detected in the blood of the animals administered 12S toxins orally or parenterally was identified as Ealpha from the molecular size and the chromatographic pattern. Prototoxin escaping from detoxification in the stomach owing to the subunit structure may undergo dissociation in the intestine to release the Ealpha component. After absorption, the activated Ealpha appeared in the circulating blood without any further signs of dissociation or enzymatic digestion.


L-malate oxidation by the electron transport fraction of Azotobacter vinelandii. The membrane-bound l-malate oxidoreductase of Azotobacter vinelandii strain O was found to be a flavoprotein-dependent enzyme associated with the electron transport system (R(3)) of this organism. The particulate R(3) fraction, which possessed the l-malate oxidoreductase, carried out the cyanide-sensitive oxidation of l-malate, d-lactate, reduced nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate, succinate, cytochrome c, tetramethyl-p-phenylenediamine, and p-phenylenediamine, with molecular O(2) as the terminal electron acceptor. d-Malate was not oxidized, but l-malate was oxidized to oxalacetate. Phenazine methosulfate (PMS), vitamin K(3), K(3)Fe(CN)(6), nitro blue tetrazolium, and dichloroindophenol all served as good terminal electron acceptors for the l-malate oxidoreductase. Cytochrome c was a poor electron acceptor. Extensive studies on the l-malate oxidase and PMS and K(3) reductases revealed that all were stimulated specifically by flavine adenine dinucleotide and nonspecifically by di- or trivalent cations, i.e., Ca(++), Ba(++), Mn(++), Mg(++), Fe(+++), Ni(++), and Al(+++). All these activities were markedly sensitive to ethylenediaminetetraacetate (EDTA). The V(max) values for the l-malate oxidase, PMS, and vitamin K(3) reductases were, respectively, 3.4, 15.1, and 45.5 mumoles of substrate oxidized per min per mg of protein at 37 C. Spectral studies revealed that the Azotobacter R(3) flavoprotein and cytochromes (a(2), a(1), b(1), c(4), and c(5)) were reduced by l-malate. l-Malate oxidase activity was sensitive to various inhibitors of the electron transport system, namely, p-chloromercuriphenylsulfonic acid, chlorpromazine, 2-n-heptyl-4-hydroxyquinoline-N-oxide, antimycin A, and KCN. Minor inhibitory effects were noted with the inhibitors 4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedione, rotenone, and Amytal.


Autolytic enzyme system of Streptococcus faecalis. V. Nature of the autolysin-cell wall complex and its relationship to properties of the autolytic enzyme of Streptococcus faecalis. Cell walls from exponential-phase cultures of Streptococcus faecalis ATCC 9790 contain an autolysin (a beta-N-acetylmuramide glycanhydrolase, E.C. 3.2.1.17) which has been isolated from trypsin-speeded wall autolysates. The autolysin, which was excluded from Bio-Gel P-60, was further fractionated by diethylaminoethyl (DEAE)-cellulose chromatography or filtration on Bio-Gel P-200. After DEAE-cellulose chromatography, which removed most of the wall polysaccharide, autolysin activity was extremely labile and was rapidly lost at -20 C, even in the presence of albumin. The P-60-excluded enzyme was rapidly bound by walls at both 37 C (50% bound in about 1 min) and 0 C (50% bound in less than 4 min). Wall-bound autolysin could not be removed by 1.0 m ammonium acetate (pH 6.9). Autolysin was also bound by walls that had been extracted with 10% trichloroacetic acid or treated with 0.01 n periodate, suggesting that the nonpeptidoglycan wall polymers are not important for binding. Wall-bound autolysin was more stable than the soluble enzyme to proteinase digestion, acetone (40%), 8 m urea (at 0 C), or to inactivation at 56 C. Two bacterial neutral proteinases (which do not hydrolyze ester bonds) activated latent wall-bound autolysin, suggesting that activation results from the cleavage of one or more peptide bonds. The group A streptococcal proteinase activated latent autolysin but differed from the other proteinases in that it did not inactivate soluble autolysin. The results suggest that the autolysin is not covalently linked to the wall. The high affinity of the walls for the autolysin appears to be responsible for the firm, not easily reversed binding.


Properties of fructose 1,6-diphosphate aldolases from spores and vegetative cells of Bacillus cereus. Fructose 1,6-diphosphate aldolase from cells of Bacillus cereus appears to be typical Class II aldolase as judged by its functional and physical properties. Spore and vegetative cell aldolase had similar enzymatic, immunochemical, and heat resistance properties in the absence of calcium, but they differed in their thermal stabilities in the presence of calcium, their Stokes' radii, their mobility in acrylamide gel electrophoresis, and their molecular weights. The pH optimum for both enzymes was 8.5, and their K(m) with respect to substrate was 2 x 10(-3)m. Highly purified spore and vegetative cell aldolases were both heat labile with half-lives of 4 min at 53 C and pH 6.4. In the presence of 3 x 10(-2)m solution of calcium ions, the stability of the spore protein increased 12-fold but the vegetative form became more heat labile. The enhanced stability of the spore aldolase was not diminished by dialysis or gel filtration but was lost after chromatography on diethylaminoethyl cellulose at pH 7.4. Aldolase from vegetative cells exists in an equilibrium mixture of two molecular weights, 115,000 and 79,000 in the approximate ratio of 1:4, respectively. The molecular weight of spore aldolase is 44,000. Spore aldolase was more mobile during electrophoresis than its vegetative cell counterpart because of its smaller size.


Physical heterogeneity among Bacillus subtilis deoxyribonucleic acid molecules carrying particular genetic markers. In a Bacillus subtilis deoxyribonucleic acid (DNA) preparation, extracted and purified by the Marmur procedure, the DNA molecules carrying a particular marker are heterogeneous with respect to molecular weight, buoyant density, and thermal stability. This finding constitutes evidence against unique points of breakage during DNA isolation. The variation in buoyant density suggests a local compositional heterogeneity in the chromosomal region of certain markers. The variation in molecular weight provides an explanation for the results of certain transformation experiments that are otherwise poorly understood. An example of such a result is the observation that acridine orange increases the efficiency of differential thermal inactivation of markers. An explanation of this phenomenon is suggested by the demonstration that acridine orange can decrease the natural intramarker heterogeneity in melting behavior.


Kinetics of induction and purification of chloramphenicol acetyltransferase from chloramphenicol-resistant Staphylococcus aureus. Plasmid-mediated chloramphenicol resistance in Staphylococcus aureus has been shown to involve acetylation of chloramphenicol by an enzyme induced by growth in the presence of the antibiotic and certain analogues. Analysis of the kinetics of induction has been complicated by (i) the intrinsic inhibitory effects of chloramphenicol on induced enzyme synthesis and (ii) the rapid disappearance of inducer after synthesis of the acetylating enzyme. The compound related to d-threo chloramphenicol which lacks a C(3) hydroxyl substituent (3-deoxychloramphenicol) is a potent inducer of chloramphenicol acetyltransferase but is ineffective as an antibiotic and is not a substrate for the enzyme. The availability of such a "gratuitous" inducer has simplified an analysis of the kinetics of induction of chloramphenicol acetyltransferase. The enzyme from induced bacteria has been purified to homogeneity and has been compared with the analogous enzyme present in E. coli which harbors a resistance transfer factor with the chloramphenicol resistance determinant.


Preparation and ultrastructure of the outer coats of Azotobacter vinelandii cysts. Cysts of Azotobacter vinelandii were ruptured with 3.0 mm ethylenediaminetetraacetate in 0.05 m tris(hydroxymethyl)aminomethane buffer (pH 7.8) and fractionated by differential centrifugation in density gradients of sucrose or Ficoll (polysucrose) at 10,000 x g for 2 hr at 4 C. The average densities of the cyst exine, the cyst central body, the vegetative cell, and the intact cyst were found to be 1.13, 1.26, 1.28, and 1.31 g/cm(3) in sucrose, and 1.08, 1.12, 1.13, and 1.17 g/cm(3) in Ficoll, respectively. These data were utilized to devise procedures for the isolation of cyst components. Cyst exine appeared as a multilayered structure in ultrathin sections. This same sheetlike structure was seen when exines were subjected to detergent treatment, metal-shadowed, and examined in an electron microscope. The exine was lysed by trypsin and was resistant to lysozyme, indicating that protein may be the principal structural material of the outer cyst coat.


Morphological studies on relaxed and contracted forms of purified pyocin particles. The bacteriocin from Pseudomonas aeruginosa, pyocin, consists of a contractile sheath and inner core reminiscent of T-even coliphage tails. Contraction of the outer sheath was found to be promoted by 0.5 m magnesium chloride, 1% Formalin, low pH, sonic treatment, and freezing or thawing or both. The contraction caused by 0.5 m magnesium chloride, however, was found to be reversible and occurred upon reduction of the salt concentration from 0.5 to 0.02 m. In addition, direct assay showed that pyocin activity was nearly proportional to the percentage of only uncontracted forms. Initial studies suggested that the adsorption of purified pyocin onto cell wall fragments from the sensitive indicator strain of P. aeruginosa occurs with the relaxed particle only and not with the contracted form. However, after adsorption, contraction occurred. Various morphological structures, such as tail fibers and base-platelike appendages, were also observed. Upon contraction, six tail fibers were observed on many particles, four of which appeared to originate from the sheath and two from the inner core. Polysheaths and polycores several hundred nanometers in length were also occasionally observed.


Linked transformation of bacterial and prophage markers in Bacillus subtilis 168 lysogenic for bacteriophage phi 105. Level of competence reached by Bacillus subtilis 168 lysogenic for temperate phage phi 105 was reduced compared to that reached by nonlysogenic cells. This effect was probably related to an alteration of the bacterial surface. Deoxyribonucleic acid extracted from phi 105 lysogenic bacteria was used to transform other lysogenic bacteria. About 25% linkage was found between the bacterial phe-1 marker and prophage marker ts N15. The order of a few prophage markers relative to phe-1 was established in three-factor crosses. The usefulness of this system for a study of the linkage between an integrated prophage genome and that of its host was discussed.


The development of the trigeminal nerve in baboon embryos (papio sp.).


Relations between coenzyme A and presumptive acyl carrier protein in different conditions of streptococcal growth. Exploration of the specific role of cystine in the postexponential growth of Streptococcus faecalis led to an inquiry into the fate of cellular coenzyme A (CoA) and acyl carrier protein (ACP), both of which depend for their biosynthesis on cystine and pantothenate as precursors. In S. faecalis cells labeled by growth in the presence of (14)C-pantothenate, the label could be separated on the basis of solubility at pH 2.1 into two fractions of sharply differing metabolic characteristics. The fractions were not purified, but the soluble (14)C behaved analytically like CoA, and the insoluble (14)C was considered to represent an ACP-like entity on the basis of circumstantial evidence. The fate of these two fractions under various conditions of growth was studied. When the medium contained an excess of the needed precursors, the cellular content of CoA and ACP appeared to remain constant during exponential growth, and in a molar ratio of about 4 CoA to 1 ACP. Cellular ACP, once formed, appeared to be stable under these conditions, but CoA was degraded and replaced at the rate of approximately 20% per division period. With restrictive levels of pantothenate in the medium, initially formed CoA disappeared during growth, as a result, apparently of being converted to ACP. However, when the resulting CoA-depleted cells were returned to a medium containing enough pantothenate, resumption of normal growth was preceded by a lag period, during which rapid conversion of ACP to CoA appeared to take place.


Projections from the cerebral cortex to intralaminar nuclei in monkey.


Relationship between th course of gametocyte appearance of Akiba caulleryi in the chicken and sporozoite production in Culicoides arakawae.


Influence of temperature on the sporogony of Akiba caulleryi in three Culicoides species.


Gentamicin: in vitro studies.

Gentamicin: in vitro studies.


Experience with gentamicin and specialty use of gentamicin. Gentamicin: laboratory and clinical experience in Mexico.


The dose, distribution, and excretion of gentamicin with speical reference to renal failure.


Gentamicin: pharmacologic observations in newborns and infants.


The eclectroretinogram: its components and their origins.


Gentamicin: clinical and laboratory studies in infants and children.


Gentamicin in the treatment of pulmonary infections.


Gentamicin in the management of thermal injuries.


Gentamicin, a new aminoglycoside antibiotic: clinical and laboratory studies in urinary tract infection.


The diagnosis and treatment of pseudomonas sepsis in major burns.


Self administration of and behavioral dependence on drugs.


Micropuncture and diuretics.

Micropuncture and diuretics.


Serum albumin, prealbumin, and postalbumin in perinatal pigs.


Action of drugs on movement of ocular fluids.


Comparative study of secondary epileptogenesis.


Experimentak kuru encephalopathy in chimpanzees and spider monkeysElectron microscopic studies.


Virus-like particles in murine ependymoblastoma.


The histochemical relationship of neuromelanin and lipofuscin.


Cause of infectious mononucleosis.

Cause of infectious mononucleosis.


Looking at alcohol.

Looking at alcohol.


The story of Credit Unions.

The story of Credit Unions.


The management of Rh iso-immunization by amniotic fluid analysis.


Non-nursing duties.

Non-nursing duties.


Launching of wastage survey.

Launching of wastage survey.


Parasitaemia in mice inoculated with T. cruzi at different ages.


The separation of trypanosomes from man and domestic stock in Nigeria.


Comparative effects of diverse mammalian sera on the uptake and protein incorporation of 14C-labelled amino acids by blood stream forms of T. brucei and T. rhodesiense in vitro.


Pathology, haematology and serology of visceral larva migrans in rabbits.


Evidence of continued presence of trypanosomes in blood after challenge with Trypanosoma cruzi.


An unidentified trypanosome, and a new Babesia sp. from wild rats from the Congo.


Two new Reduviidae: Triatoma lenti and T. pessoai.


The normal course of infection of Plasmodium falciparum in owl monkeys.


An outbreak of tuberculosis in experimental monkeys caused by the bovine organism.


Vaccination against P. falciparum in owl monkeys.


The pathology of Plasmodium falciparum in owl monkeys.


Active polypeptides in malaria responsible for increase in capillary permeability.


Immunological studies with simian malarias. I. Antigenic variants of Plasmodium cynomolgi bastianellii.


Some aspects of the antimalarial action of chloroquine.


Immunological studies with simian malarias. II. Heterologous immunity in the "cynomolgi" group.


Chagas's disease in British Honduras.

Chagas's disease in British Honduras.


Mechanisms of two- and four-electron oxidations catalyzed by some metalloenzymes.


Liver involvement in acute mammalian malaria with special reference to Plasmodium knowlesi malaria.


Experimental chemotherapy of Schistosomiasis mansoni.


Light and electron microscopy of the adrenal medulla of Macaca mulata monkey.


The reduction of ferricytochrome c during the enzymic oxidation of 3,4-dihydroxyphenylalanine.


Sensitivity of Escherichia coli to viral nucleic acid. I. Effect of lysozyme, EDTA, penicillin and osmotic shock treatment.


Serial complement component alterations in acute glomerulonephritis and systemic lupus erythematosus.


Inorganic sulfate-induced swelling in isolated rat-liver mitochondria.


Ovarian hormones and sexual preference in rhesus monkeys.


Characterization and properties of a new placental protein.


Metabolism of thiazole acetic acid derivatives and the NIH shift.


2-(4-chlorophenyl)thiazol-4-ylacetic acid('Myalex'): a new compound with anti-inflammatory,, analgesic and antipyretic activity.


Interoceptive Sd of the large intestine established by mechanical stimulation.


Immunochemical studies on the lipid-protein complex. I. The lipid-containing C-reactive protein.


Organ transplantation.

Organ transplantation.


The influence of methylene blue on the pentose phosphate pathway in erythrocytes of monkeys infected with Plasmodium knowlesi.


Scleroderma. 1. Clinical features, course of illness and response to treatment in 61 cases.


Contribution of cones to the early receptor potential in the rhesus monkey.


Urban monkeys.

Urban monkeys.


Lack of correlation between DAO inhibitory strength and catatonia-inducing property of aminoguanidine.


Pathomorphology of experimental head injury due to rotational acceleration.


Incorporation of radioactivity from 14C-nitroglycerin into rat liver glycogen, lipid, protein, ribonucleic acid and deoxyribonucleic acid.


Search for specific brucella agglutinins in goat's milk.


Citromycin, a new antibiotic. I. Isolation and characterization.


Effect of exogenous ATP on the volume of TA3 ascites tumor cells.


Identification of human sperm-coating antigen.


Action of various agents on rabbit blastocysts in vivo and in vitro.


Thymic neoplasm: a surgical enigma.

Thymic neoplasm: a surgical enigma.


Cancerous and precancerous lung changes: a slide review.


The possibilities of a vaccine for cancer.


The future of clinical pharmacology.

The future of clinical pharmacology.


Malaria chemoprophylaxis with 4,4' diaminodiphenylsulfone (DDS). I. Field trial with comparison among companies of one division.


Clinical staging of cancer and end result reporting.


Maintaining the oral and general health of the oral cancer patient. 1.


Unproven methods of cancer treatment.

Unproven methods of cancer treatment.


Malaria chemoprophylaxis with 4,4' diaminodiphenylsulfone (DDS). II. Field trial with comparison between two divisions.


A life with cancer: a physician's personal experience.


Prediction of trainee effectiveness in an army training center.


Paroxysmal tachycardia of infancy.

Paroxysmal tachycardia of infancy.


Abnormal siblings in patients with trisomy 13-15 and 17-18.


Management of combined intracranial injuries and extensive orbital-facial fractures.


Lateral sinus compression from an epidural hematoma: report of a patient with delayed neurological deterioration.


Dystocia secondary to a sacrococcygeal teratoma. Report of a case.


Career recruitment of military physicians.


The mycoflora of the subhuman primates. IV. The flora of the ear, nose, and intestinal contents of the baboon (Papio sp.).


Concentration of serum albumin in vascular and extravascular tissue pools of the mouse after x-irradiation.


Congenital body malformations and eye color mutations in progeny from irradiated female wasps (Dahlbominus).


Abnormal development of eyes in mice homozygous for the fidget gene.


Cell cultures useful for the study of rubella.


Epidemiology of rubella. Subclinical infection and occurrence of reinfection.


Direct hemadsorption by cell cultures infected with rubella virus.


Antigens of rubella virus.

Antigens of rubella virus.


Bacteriocins from human and rodent streptococci.


Comparison of sucrose and glucose in the causation of dental caries in gnotobiotic rats.


The relation of migraine and epilepsy.

The relation of migraine and epilepsy.


The effects of radical disconnexion of occipital and temporal cortex on visual behaviour of monkeys.


Pain in the ankle.

Pain in the ankle.


Electron microscopy of micro-focal necrosis in malignant melanomas.


Purification and immunochemical analysis of a genus-specific cuticular antigen of Toxocara canis.


Effect of heterologous antibody on Haemonchus contortus development in vitro.


Cultivation of Besnoitia jellisoni in bovine cell cultures.


Effects of 8-azaguanine on the pathogenesis of experimental Chagas' disease.


Relation of Australia-SH antigen to the willowbrook MS-2 strain.


Beta adrenergic blockade and juvenile diabetes: acute studies and long-term therapeutic trial. Evidence for the role of catecholamines in mediating diabetic decompensation following emotional arousal.


Current problems in health care.

Current problems in health care.


Malignant lymphoma of Waldeyer's ring.

Malignant lymphoma of Waldeyer's ring.


Angiographic changes following acute experimental cerebral embolism in monkeys. Preliminary report.


Protective effects of arginine against hepatic coma, insulin shock and epilepsy.


The management of acid-base balance in children with diabetic acidosis.


The effect of an intra-uterine device on uterine motility in women and rhesus monkeys.


The laboratory management of long-term intravenous therapy.


Extended surgical adjuvant chemotherapy for breast carcinoma.


Haemodynamic observations during spontaneous angina pectoris.


Study on heat labile somatic antigens of Listeria monocytogenes.


Change: what kind? how much?

Change: what kind? how much?


Amyloid arthritis associated with Waldenstrom's macroglobulinemia.


Twin pregnancy and erythroblastosis.

Twin pregnancy and erythroblastosis.


Petit mal seizures.

Petit mal seizures.


A large-vessel applique A-V shunt for hemodialysis.


Internal arteriovenous fistulae in a hemodialysis-transplant program.


Albumin turnover in chronically hemodialyzed patients.


Comparative performance characteristics of l3 hemodialyzers.


Increased antibacterial resistance and immunodepression during graft-versus-host reactions in mice.


Nuclear bodies in keratosis arsenicalis.


Food supply as a factor in the survival of frozen thawed and rotifers.


An electron microscopical comparison of primary and secondary demyelination in the rat central nervous system.


Plasticity and biological activity of the intrahepatic portal bed.


Morphology of functional relations between the arteria hepatica and the vena portae.


The adrenal gland of the camel (Camelus dromedarius). A study of the comparative anatomy and lipoids.


Dynamics of the activity of aspartate aminotransferase (GOT)(2.6.1.1) and alanine aminotransferase (GPT)(2.6.1.2) in the m. pectoralis thoracicus of WL chickens.


Column chromatography of RNA and oligonucleotides on denatured proteins.


PPD antigens and the diagnosis of mycobacterial diseases. A study of atypical mycobacterial disease in Oklahoma.


Double spike in myeloma serum due to retention of light chains.


Enzymes of the tryptophan operon of Bacillus subtilis.


In vitro hybridization of mouse phosphoglucose isomerase variants.


Fructose diphosphate aldolase of yeast: studies on the sulfhydryl groups.


A micro-method for the quantitative determination of deoxyribonuclease.


On the fatty acids of Brucella abortus and Brucella melitensis.


The selective transmission of bovine gamma-globulins across the gut of suckling rodents.


Double diffusion in agarose: precipitin lines which are not the result of antigen--antigody reactions.


Comparison of the mechanisms underlying carbohydrate intolerance in subclinical diabetic women during pregnancy and during post-partum oral contraceptive steroid treatment.


The effect of cytotoxic agents on the passive transfer of cell-mediated immunity. A system involving the passive transfer of committed lymphoid cells from Listeria-immune donors has been used to study the phases of the immune response which are sensitive to the immunosuppressive action of various cytotoxic agents. The agents investigated included cyclophosphamide, vinblastine, methotrexate, azathioprine, and X-irradiation. Complete suppression of passive immunization was obtained by the administration of cyclophosphamide or vinblastine to recipients at the time of cell transfer or by prior X-irradiation of recipients a day before cell transfer. Methotrexate was only partially suppressive, whereas azathioprine had no effect at all. The donor cell responsible for the transfer of immunity to recipients was shown to be a resting cell which is sensitive to the action of cyclophosphamide but not to vinblastine. The results of this investigation suggest that the donor cells undergo multiplication in the tissues of the recipient, presumably in response to specific stimulation by Listeria antigens. This in turn results in the activation of host macrophages. The immunosuppressive action of cyclophosphamide, vinblastine, and irradiation in the cell-transfer system has been discussed in relation to a direct cytotoxic action on the immune lymphoid cells of the donor and specific interference with their proliferation in the recipient, as well as impairment of macrophage production on the part of the recipient itself.


The IgG receptor: an immunological marker for the characterization of mononuclear cells.


The effect of cytotoxic agents on the primary immune response to Listeria monocytogenes. Four drugs, representing four different categories of cytotoxic agents, were studied for their effect on the immune response to Listeria infection in mice. The development of the host's immune response is revealed by a progressive change in the slope of the bacterial growth curve in spleen and liver. It has its onset at 24 hr in untreated mice, but in the presence of effective immunosuppression the organism multiples uninterruptedly until the animal dies from overwhelming infection. When administered as single injections at the time of infection, cyclophosphamide, vinblastine, and azathioprine all produced an effective immunosuppression, characterized by continuous bacterial multiplication. Methotrexate was also immunosuppressive, but unlike the others its effects were reversible. They could be sustained, however, by further treatment. Studies of the time-response relationship indicated that cyclophosphamide was highly active over a broad time-span ranging from 2 days before infection to 4 days after infection. Vinblastine on the other hand was maximally active when given on the day of infection, while methotrexate had its greatest effect when given 48 hr after infection. These differences indicate that these three drugs act on different cell populations involved in the host's immune response. The effects observed have been discussed in relation to what is known of the modes of action of the drugs tested. An observation of interest was the phenomenon of enhanced immunity in animals treated with cyclophosphamide or vinblastine 7-11 days before, and with methotrexate 4 days before infection; reactive hyperplasia of lymphoid tissue following withdrawal of drug was again advanced as an explanation for the occurrence of this paradoxical effect. The experimental model employed is simple, requiring only routine bacteriological facilities and minimal equipment. It seems to offer a useful means of assessing the immunosuppressive activity of drugs and of determining the time-course of their action; it could also be of value in the screening of anticancer agents.


Separation of morphine-like effects by optical resolution. Levo isomers as strong analgetics and narcotic antagonists.


Baboon kidney excretion of 203Hg-chlormerodrin during isolated bloodless perfusion.


In vivo and in vitro effects of cadmium chloride on carbonic anhydrase activity.


A preliminary study of injury and recovery of the male germinal epithelium and spermatogenesis in Macaca mulatta following whole body cobalt-60 gamma irradiation.


Penicillium cyaneo-fulvum virus and interferon stimulation.


Prostaglandin antagonists: synthesis and smooth muscle activity.


The response of the arachnoid villus to an intracisternal injection of autogenous brain tissue. An electron microscopic study in the macaque monkey.


Chronic aerogenic tuberculosis in mice.

Chronic aerogenic tuberculosis in mice.


Detection of antibody to denatured DNA in NZB/B1 mice.


Hyperpotentiation by synthetic double-stranded RNA of antibody responses to influenza virus vaccine in adjuvant 65.


Nucleic acid content of visna virus.

Nucleic acid content of visna virus.


The possibility that a large Babesia species exists in British cattle.


Binding of the 51Cr complex of ethylenediamine tetraacetic acid to particulate matter in the rumen.


Comparison of cephalothin, cephaloridine, cephalexin and cephaloglycin in streptococcal infections in monkeys.


Some aspects of morphogenesis and fruiting body formation in myxobacteria.


Report of an aberrant case of nevo-xantho-endothelioma.


On the care of the mentally subnormal.

On the care of the mentally subnormal.


Microchemical investigation of medicinal plants. I. The antitubercular principle in Prunus mume and Schizandra chinensis.


Microchemical investigation of medicinal plants. II. The antitubercular activity of some plant acids and their hydrazides.


Renal tubular acidosis in an adult.

Renal tubular acidosis in an adult.


Alterations in water intake and core temperature in baboons during hypothalamic thermal stimulation.


The young diabetic enters college.

The young diabetic enters college.


Effects of spatial and nonspatial distractors on performance latency of monkeys with frontal lesions.


Vagal compensatory adjustment: relationship to heart rate classical conditioning in rabbits.


New concepts in facial bone healing and grafting procedures.


Facial elongation after treatment of horizontal fracture of the maxilla without vertical suspension.


Condyle fractures.

Condyle fractures.


Experimental fractures of the mandible.

Experimental fractures of the mandible.


Vascular changes in bone repair.

Vascular changes in bone repair.


Barbiturate overdosage: presentation with focal neurological signs.


Content and release of amino-acids and catecholamines in monkey brain.


Defective bactericidal activity in myeloperoxidase-deficient human neutrophils.


Persistence of the serum hepatitis (SH-Australia) antigen for many years.


Determination of cesium 137 in waters.

Determination of cesium 137 in waters.


External morphology of the brain of the givet monkey (Cercopithecus aethiops aethiops).


The structure of the palatum osseum and its toral overgrowths.


The three dimensional architecture of the islets of Langerhans.


Juxtacardial gastric diverticula and their surgery.


Immunological and physico-chemical study of rat urinary proteins.


Biologic studies on the relationship between thyroid stimulating and ophthalmotrophic activities.


Studies on a human 19-S immunoglobulin M. The arrangement of inter-chain disulphide bridges and carbohydrate sites.


Reduction of allergic encephalomyelitis incubation period to five days.


The accessory optic system and other optic fibers of the squirrel monkey.


In vivo behaviour of frozen and freeze-dried fibrinogen and of that prepared from out-dated blood.


Phosphoglucomutase. VI. Purification and properties of phosphoglucomutases from human muscle.


Sporulation in Bacillus subtilis. Establishment of a time scale for the morphological events.


The interaction of thyroxine with human plasma prealbumin and with the prealbumin-retinol-binding protein complex.


The multiple forms of mushroom tyrosinase. Structural studies on the isozymes.


Production of adenosine triphosphate in normal cells and sporulation mutants of Bacillus subtilis.


Properties of Proteus mirabilis and Providence spheroplasts.


Smooth muscle relaxants: dissociation between resting membrane potential and resting tension in rat myometrium.


The pharmacology of 2-(ethylamino)-2-(2-thienyl)-cyclohexanone-HCl (CI-634).


Uptake of chloroquine-3-H3 by plasmodium knowlesi in vitro.


A comparison of the effects of various smooth muscle relaxants on the electrical and mechanical activity of rat uterus.


Effect of nitrates and other coronary dilators on large and small coronary vessels: an hypothesis for the mechanism of action of nitrates.


Degradation of human IgM anti-gamma-globulin by pepsin provides evidence of an active fragment.


Rubidium: a potential modifier of affect and behaviour.


The autoxidation of haemoglobin. Effect of copper.


Vitamin E and the biological antioxidant theory.


Serial hormonal studies in normal and abnormal pregnancy.


Quantitive measurements of DNA content of cervical cancer cells before and after test dose radiation.


Estrogen-induced changes at the cervical squamocolumnar junction of the squirrel monkey.


Glucose concentration gradients across the uterus and placenta of the pregnant primate.


Feline urethral obstruction: immunologic identification of a unique urinary protein.


Heterotopic bone formation in tuberculous salpingitis.


Certain characteristics of viruses isolated from turkeys with bluecomb.


Characterization of pathogenic filtrate and viruses isolated from turkeys with bluecomb.


Intra-sexual behaviour and female reproductive cycles of baboons (Papio anubis).


Aggressive behaviour of the eight-year-old nulliparous isolate female monkey.


Virulence of gonococci.

Virulence of gonococci.


Synthesis of milk proteins in a cell-free system isolated from lactating bovine mammary tissue.


Experimental allergic encephalomyelitis. An encephalitogenic basic protein from bovine myelin.


Automatisms associated with the absence of petit mal epilepsy.


The study of toluidine blue-ribosomal RNA complexes.


Light-scattering studies on rabbit brain microsomes. II. Efects of ATP and chelation of Mg2+ on microsomal contraction.


The chemical basis of the virulence of Brucella abortus. IX. The increased immunogenicity of Br. abortus grown in media which enhance its ability for survival within bovine phagocytes.


Carbenicillin resistance in Pseudomonas aeruginosa from clinical material. Strains of Pseudomonas aeruginosa resistant to 256 mug. per ml. or more of carbenicillin (Pyopen) were isolated from 17 patients over a period of three months. The infections were not solely due to cross-infection. Low dosage and attempted eradication of the organism from inaccessible sites, such as the bronchi or skin surfaces, by using systemic treatment are two possible causes. Restraint in treating infections of doubtful importance and the use of local applications to appropriate sites with or without systemic treatment is suggested.


The doublet pattern of the nucleic acid of oncogenic and non oncogenic viruses and its relationship to that of mammalian DNA.


Human tumour antigens.

Human tumour antigens.


Kommerell's diverticulum with esophago-arterial fistula.


Treatment of protein losing enteropathy due to idiopathic intestinal lymphangiectasis by parenteral nutrition.


Effect of metal ions on estradiol binding to uterine nuclear receptors.


Effect of insulin on the carbohydrate metabolism of fetal rhesus monkey muscle.


Congenital hemihypertrophy with neurofibromatosis.


Acute volvulus of the small bowel due to multiple jejunal diverticula.


Eight years' experience using an aseptic air system in surgery.


Antimicrobial proteins isolated from the teat canal of the cow.


Viable bacteria inside the rumen ciliate Entodinium caudatum.


The histochemical tyrosine-dopa reaction for tyrosinase and its use in localizing tyrosinase activity in mast cells.


Repository drugs. VI. 4'-[N-(Aralkylidene-, -Benzylidene-, and -naphthylidene)sulfanilyl]anilides, 4'-[N-[(Dimethylamino)methylene]sulfanilyl]anilides, and related sulfanilylanilides with prolonged antimalarial and antileprotic action.


N4, N4'-Decamethylenebis-4-aminopyridine and N9, N9'-decamethylenebis-9-aminoacridine.


N1-sulfato-N2-isonicotinylhydrazine. A potential metabolite of isoniazid.


Preparation and antibacterial activity of alpha-(5-tetrazolyl) benzylpenicillin.


Nitroheterocycles. I. Nitrofuryl-substituted 3-amino-1,2,4-oxadiazoles and 5-amino-1,2,4-oxadiazoles.


Nitrones. I. alpha-(5-Nitro-2-furyl)-N-arylnitrones.


A perspective on Medicaid.

A perspective on Medicaid.


Evaluation of isoniazid administration on pulmonary tuberculous patients with isoniazid-resistant strain.


Atlanto-epistrophical subluxation in rheumatoid arthritis.


Meningitis due to listeria monocytogenes.


Chronic actinomycosis treated by oral penicillin.


Significance of Ps. aeruginosa in rice watery stool.


Systemic alkalosis in hypoparathyroidism.


Effect of an intra-uterine device on endometrial morphology and the deciduomal reaction in the Rhesus monkey.


Some critical factors in double antibody radioimmunoassay systems utilizing sheep anti-rabbit precipitating sera for measurement of human serum LH, FSH and HGH.


Effect of an intra-uterine device on uterine motility in the Rhesus monkey.


Effects of clofibrate (Atromid S) on the thyroxine-binding capacity of thyroxine-binding globulin and free thyroxine.


Corticosteroid-induced cataract and palatoschisis in the mouse fetus.


Experimental retinal detachment and reattachment in the rhesus monkey. Electron microscopic comparison of rods and cones.


Surgical treatment of blepharoptosis.

Surgical treatment of blepharoptosis.


Utilization and price analysis: prospects for avoiding higher program costs in health care.


Primary drug resistance: A continuing study of tubercle bacilli in a veteran population within the United States. VI. Initial observations on the incidence of resistance to rifampin and ethambutol.


Mechanism of uptake of purines by Pseudomonas aeruginosa.


The antigenicity of human hemoglobins. II. The correlation of the antigenicity among alpha, beta, gamma-chains and native hemoglobins.


The monitoring of acid production in dental plaque in monkeys.


The healing of extraction wounds.

The healing of extraction wounds.


Fluorescent antibody and agar gel diffusion techniques to detect Mycoplasma mycoides in fresh and formalin-fixed lung lesions of cattle.


A virus associated with pseudorinderpest in Nigerian dwarf goats.


Resistant and sensitive strains of Mycobacterium tuberculosis found in repeated surveys among a South Indian rural population. The findings in a highly selected group of patients, such as those attending clinics or sanatoria, cannot be used as the basis for assessing the true prevalence of strains of Mycobacterium tuberculosis with acquired or primary resistance or of sensitive strains in a community. The present report describes the prevalence of such strains as found in 3 successive surveys in a sizeable random sample of villages in a South Indian district. Changes in the status of cases with such strains from an earlier survey to a later one and the status at an earlier round of cases found at a later one are also described.The prevalence of tuberculous infection among household contacts of cases with acquired resistance to isoniazid was significantly higher than that among contacts of cases with primary resistance or of those with sensitive cultures. This is probably due to the longer duration of sputum positivity of the former at the time of diagnosis. But infectivity, as judged by the incidence of new infections among household contacts, was generally less for cases with acquired or primary resistance than for cases with sensitive cultures, though the difference was not statistically significant.A large number of culture-positive cases, especially those with primary resistance, had no radiological evidence of active pulmonary tuberculosis. The prevalence of primary resistance was very high among certain categories of cases, and the differences between cases with primary resistance and those with acquired resistance were many and large. It is suggested that this could be due to some of the primary resistant cultures being those of atypical mycobacteria, despite positivity in the niacin test.There was a significant increase in the number of cases with acquired resistance to isoniazid at the third survey round owing to irregular treatment with that drug after the second round. The prevalence of primary resistance at the 3 rounds was almost the same.


Some actions of hormones in the regulation of glucose metabolism.


Advances in medical care of the elderly.


Long-term office treatment of Meniere's disease.


Neurofibromatosis with paraplegia. Report of eight cases.


Open skin grafting of war wounds.

Open skin grafting of war wounds.


Radiostimulation of the brain in primates and man. Fourth Becton, Dickinson and Company Oscar Schwidetzky Memorial Lecture.


Effects of serial disconnection of striate and temporal cortex on visual discrimination performance in monkeys.


Analysis of changes in indirect delayed response performance in monkeys with prefrontal lesions.


Detoxification of an immunogenic fraction from a culture filtrate of Pseudomonas aeruginosa.


Sulfameter (Sulla).

Sulfameter (Sulla).


Sulphadiazine-resistant group A meningococci isolated during the 1968 meningitis epidemic in Greece.


Effect of an intra-uterine device on conception and ovulation in the Rhesus monkey.


Effect of an intra-uterine device on menstrual periodicity in the Rhesus monkey.


Effect of an intra-uterine device on gamete transport and fertilization in the Rhesus monkey.


Latent gonorrhea in women.

Latent gonorrhea in women.


A test of platelet adhesiveness.

A test of platelet adhesiveness.


Merozygotic processes, microorganisms, and neoplastic etiology.


Roentgenographic appearance of the transplanted primate lung. The autotransplanted lung.


Massive hemorrhage from jejunal diverticulosis.


Studies on African Pygmies. I. A pilot investigation of Babinga Pygmies in the Central African Republic (with an analysis of genetic distances).


Dimethyl sulfoxide (DMSO)--current concepts in toxicology, pharmacology, and clinical usefulness in surgery.


Experimental gastritis in the dog II. Production of antibodies to a gastric B12-binding auto-antigen.


The effects of some alpha picolinic acid derivatives on growth and sporulation of bacilli.