Observations related to pathogenesis of dengue hemorrhagic fever. I. Experience with classification of dengue viruses.


Susceptibility in vitro of Haemophilus influenzae to penicillin G, penicillin V and ampicillin. Incubation of strains from acute otitis media in air and in CO2-atmosphere.


Endotoxin-induced cytotoxicity of rabbit serum.


Serological studies of a collection of strains of Moraxella species and related organisms.


The serology of Moraxella kingii.

The serology of Moraxella kingii.


Effect of basic cupric acetate on the biochemical changes in the liver of the rat fed carcinogenic aminoazo dye. II. Activity and isozyme pattern of lactate dehydrogenase.


Osmotic properties of spheroplasts from Saccharomyces cerevisiae grown at different temperatures. Spheroplasts were prepared from cells of Saccharomyces cerevisiae NCYC 366, grown at 30 or 15 C, by incubating cells with snail-gut juice after pretreatment with 2-mercaptoethanol. Walls of cells grown batchwise or in continuous culture at 15 C were more resistant to digestion with snail juice than walls on cells grown under the same conditions as 30 C. Spheroplasts lysed when suspended in hypotonic solutions of mannitol. The resistance of spheroplasts to osmotic lysis tended to increase when the test temperature was lowered below 30 C. The increased resistance was greater with spheroplasts from cells grown at 15 C. Cations, especially Ca(2+), protected spheroplasts against osmotic lysis. In general, the protective effects, measured at 30 C, were smaller with spheroplasts from cells grown at 15 C compared with 30 C. Citrate and ethylenediaminetetraacetate (EDTA) decreased the resistance of spheroplasts to osmotic lysis. On the whole, the decrease was greater with spheroplasts from cells grown at 30 C rather than 15 C. In the presence of EDTA, spheroplasts from cells grown at 30 C were less resistant to osmotic lysis at 5 C than at 30 C; when spheroplasts from cells grown at 15 C were similarly examined, they were more resistant to lysis at 5 C than at 30 C. Spheroplast membranes from cells grown at 15 C had slightly but significantly greater contents of Mg(2+), Ca(2+), K(+), and Na(+) compared with spheroplast membranes from cells grown at 15 C. Mg(2+) and Ca(2+) were more easily extracted with EDTA from membranes of 30 C-grown cells than from 15 C-grown cells.


Carbon dioxide fixation by extracts of Streptococcus faecalis var. liquefaciens. Extracts of cells of Streptococcus faecalis var. liquefaciens strain 31 incorporated (14)CO(2) into aspartate. Dialyzed extracts produced radioactive oxalacetate in the absence of exogenously added glutamate and pyridoxal-5'-phosphate and produced radioactive aspartate in the presence of these components. Reduced nicotinamide adenine dinucleotide or reduced nicotinamide adenine dinucleotide phosphate could not be substituted for adenosine triphosphate (ATP); phosphoenolpyruvate even in the presence of nucleoside diphosphates could not replace pyruvate plus ATP; propionate plus coenzyme A (CoA) could not replace pyruvate in supporting CO(2) fixation by cell extracts. Fixation by dialyzed cell extracts required pyruvate, ATP, MgSO(4), and was stimulated by biotin, KCl, 2-mercaptoethanol, CoA, and acetyl CoA. Inhibition of fixation occurred when avidin, NaCl, oxalacetate, or aspartate was added to dialyzed extracts. On the basis of the products formed and the effects of substrates and cofactors on the fixation reaction, it was concluded that pyruvate carboxylase is responsible for CO(2) fixation in this microorganism.


Comparative immunological studies of two Pseudomonas enzymes. Crystalline preparations of muconate lactonizing enzyme and muconolactone isomerase, two inducible enzymes that catalyze successive steps in the catechol branch of the beta-ketoadipate pathway, were used to prepare antisera. Both enzymes were isolated from a strain of Pseudomonas putida biotype A. The antisera did not cross-react with enzymes of the same bacterial strain that catalyze the chemically analogous steps in the protocatechuate branch of the beta-ketoadipate pathway, carboxymuconate lactonizing enzyme and carboxymuconolactone decarboxylase. The antisera gave heterologous cross-reactions of varying intensities with the muconate lactonizing enzymes and muconolactone isomerases of P. putida biotype B, P. aeruginosa, P. stutzeri, and all biotypes of P. fluorescens, but did not cross-react with the isofunctional enzymes of P. acidovorans, of P. multivorans, and of two bacterial species that belong to other genera. The evolutionary and taxonomic implications of the findings are discussed.


Formation of protoporphyrin from coproporphyrinogen in extracts of various bacteria. The enzyme system capable of converting coproporphyrinogen to protoporphyrin was demonstrated in the soluble fraction of extracts of Pseudomonas fluorescens grown aerobically, of P. denitrificans grown anaerobically under denitrifying conditions, and of Escherichia coli grown both aerobically and anaerobically. Protoporphyrin accumulation by each of these extracts occurred only if the assay was conducted aerobically. Attempts to replace this oxygen requirement with several alternate electron acceptors were not successful. The conversion of coproporphyrinogen to protoporphyrin could not be demonstrated in extracts of the heme-containing organisms Staphylococcus epidermidis and Bacillus subtilis.


Relative affinity of vancomycin and ristocetin for cell walls and uridine diphosphate-N-acetylmuramyl pentapeptide. A determination of the relative affinity of vancomycin and ristocetin for isolated cell walls and for a peptidoglycan precursor was made. These antibiotics had previously been shown to adsorb to cell walls and to complex with peptides containing a d-alanyl-d-alanine C-terminus. By using (14)C-uridine diphosphate (UDP)-N-acetylmuramyl pentapeptide, it was shown that the complex which is formed between this peptidoglycan precursor and either vancomycin or ristocetin does not preclude adsorption of the antibiotics to cell walls of Micrococcus lysodeikticus. Complex formation between ristocetin and UDP-N-acetylmuramyl pentapeptide was assured by differential absorption spectra. However, when the complex was mixed with cell walls, the antibiotic was sedimented with the walls, and the radioactivity remained in the supernatant solution. This indication that ristocetin and vancomycin have a greater affinity for walls than for UDP-N-acetylmuramyl pentapeptide and that the complex per se does not bind to cell walls suggests that adsorption of these antibiotics to cell walls is probably responsible for the inhibition of peptidoglycan synthesis. This proposal is strengthened by the observation that complexed antibiotic is no less inhibitory for growth of Bacillus subtilis than free vancomycin or ristocetin.


Heterogeneity of phospholipid composition in the bacterial membrane. Heterogeneity in the distribution or binding of the membrane phospholipids was demonstrated in the membrane fragments released from Haemophilus parainfluenzae by treatment with ethylenediaminetetraacetic acid (EDTA)-tris(hydroxymethyl)-aminomethane (Tris). The membrane fragments released early in the EDTA-Tris treatment contained two- to fivefold higher proportions of cardiolipin and phosphatidylglycerol and less phosphatidylethanolamine as well as phospholipids with threefold lower specific activity of the phospholipid phosphate after a short pulse of (32)P than were found in the residue. Heterogeneity was best demonstrated with shorter EDTA-Tris treatments and shorter periods of growth with (32)P. EDTA-Tris treatment appeared to progressively strip phospholipids from the cells that were synthesized at progressively later times.


Relation of lipopolysaccharide and fatty acid ester release to the ethylenediaminetetraacetic acid alteration of permeability in enterobacteriaceae. Escherichia coli subjected to cold osmotic shock released 30 to 40% of their fatty acid esters and 42% of their cellular hexosamine. In contrast, Enterobacter, although they released 40% of fatty acid esters, release only 25% of hexosamine. Proteus released less than 15% of either fatty acid esters or hexosamine. These differences are taken to explain the differences among the Enterobacteriaceae in releasing surface enzymes after osmotic shock. It is felt that the release of additional lipopolysaccharide after osmotic shock is necessary for the release of surface enzymes that are not freed by ethylenediaminetetraacetic acid-tris(hydroxymethyl)aminomethane exposure.


Transduction in Bacillus cereus by each of two bacteriophages. The ability of phage CP-51 to mediate transduction both homologously and heterologously in some of its hosts was investigated. CP-51 was shown to transduce Bacillus cereus strains 6464, 9139, and T in addition to 569 which was reported earlier from this laboratory. Furthermore, CP-51 grown on B. thuringiensis was shown to transduce some mutants of B. cereus. During this investigation, a second transducing phage for B. cereus 569 was isolated from lysates of phage CP-51 grown on B. cereus 6464. This phage, designated CP-53, is carried by wild-type strain 6464 possibly as prophage. All auxotrophic mutants of B. cereus 569 tested, those requiring tryptophan, histidine, methionine, and leucine, were transduced to prototrophy by CP-53. Electron micrographs of the two phages revealed that CP-51 has a tail core surrounded by a contractile sheath and CP-53 has a long flexible tail without a contractile sheath. CP-53 is stable in the cold, whereas CP-51 is rapidly inactivated at 4 C.


Actinomycin resistance and actinomycin excretion in a mutant of Escherichia coli. A mutant of Escherichia coli resembles its parent in taking up actinomycin after treatment with ethylenediaminetetraacetic acid but differs in that it survives this uptake and excretes actinomycin at an increased rate.


Tissue- and species-specific antigens of normal human prostatic tissue.


Yields of Hydrogenomonas eutropha from growth on succinate and fumarate. Molar growth yields were determined from chemostat cultures of Hydrogenomonas eutropha on succinate and on fumarate. The yields from culture on succinate were about 12 g higher than on fumarate. Assuming this difference to be equivalent to 1 molecule of adenosine triphosphate, it is concluded that the oxidation by oxygen of the Hydrogenomonas cytochrome b yields 1 molecule of adenosine triphosphate.


The preparation of human kininogen and the elicitation of antibody for use in a radial immuno-diffusion assay.


Dissociation of antibody production from disease suppression in the inhibition of allergic encephalomyelitis by myelin basic protein.


Localization of glycosphingolipids in human tissues by immunofluorescence.


The effect of x-irradiation on delayed hypersensitivity and circulating antibody in the rat.


Why does medical care cost so much?

Why does medical care cost so much?


Management of diabetes mellitus and surgery.


Phenylketonuria and glutamine.

Phenylketonuria and glutamine.


Single-dose antibiotic treatment of asymptomatic gonorrhea in hospitalized women.


Transmission of Theileria annulata by the crushed infected unfed Hyalomma dromedarii.


Tay-sachs disease. Detection of heterozygotes and homozygotes by serum hexosaminidase assay.


Immunization against schistosome infection in rhesus monkeys with living worms by intraperitoneal injection.


The use of parasympathomimetic drugs to stimulate salivation in the tick Rhipicephalus appendiculatus, and the transmission of Theileria parva using saliva obtained by this method from infected ticks.


Further observations on the malaria parasite of the Himalayan flying squirrel.


Some factors affecting the cultivation in vitro of the erythrocytic stages of Plasmodium knowlesi.


The use of proprietary tissue-culture media for the cultivation in vitro of the erythrocytic stages of Plasmodium knowlesi.


Population structure of blood groups in Central and South American Indians.


Dental and periodontal diseases and abnormalities in wild-caught marmosets (primates--Callithricidae).


Blood typing studies in American Indians: misclassification of RZ phenotypes.


Antigenic analysis of the developmental stagesof Ascaris suum. II. Host components.


Plasmodium inui: serologic relationships of Asian isolates.


Hypertension in a case of von Recklinghausen's neurofibromatosis.


Regular operating and sponsored program medical school expenditures 1958-1959 to 1965-1966.


Evaluation of common germicidal agents for surgical scrubs.


Specifications for the identity and purity of food additives and their toxicological evaluation: some food colours, emulsifiers, stabilizers, anticaking agents, and certain other substances. Thirtenth report of the Joint FAO-WHO Expert Committee on Food Additives. Rome, 27 May-4 June 1969.


WHO Expert Committee on plague. Fourth report.


Action of mycobacillin on filamentous fungi.


Shwartzman activity of endotuberculin preparations isolated from the cells of BCG substrains.


Effect of herbicides on growth and nitrogen-fixation of alga, Tolypothrix tenuis.


Studies on the distribution of anti-Salmonella pullorum agglutinins in blood sera and in milk of cattle.


Uptake of some catecholamines and their precursors into neurons of the rat ciliary ganglion.


Adrenergic and cholinergic nerve terminals in skeletal muscle vessels.


Species distribution of sympathetic cholinergic vasodilator nerves in skeletal muscle.


Enigmatic pneumobilia.

Enigmatic pneumobilia.


Biosynthesis of phytoquinones. Homogentisic acid: a precursor of plastoquinones, tocopherols and alpha-tocopherolquinone in higher plants, green algae and blue-green algae. 1. By means of (14)C tracer experiments and isotope competition experiments the roles of d-tyrosine, p-hydroxyphenylpyruvic acid, p-hydroxyphenylacetic acid, phenylacetic acid, homogentisic acid and homoarbutin (2-methylquinol 4-beta-d-glucoside) in the biosynthesis of plastoquinones, tocopherols and alpha-tocopherolquinone by maize shoots was investigated. It was established that d-tyrosine, p-hydroxyphenylpyruvic acid and homogentisic acid can all be utilized for this purpose, whereas p-hydroxyphenylacetic acid, phenylacetic acid and homoarbutin cannot. Studies on the mode of incorporation of d-tyrosine, p-hydroxyphenylpyruvic acid and homogentisic acid showed that their nuclear carbon atoms and the side-chain carbon atom adjacent to the nucleus give rise (as a C(6)-C(1) unit) to the p-benzoquinone rings and nuclear methyl groups (one in each case) of plastoquinone-9 and alpha-tocopherolquinone and the aromatic nuclei and nuclear methyl groups (one in each case) of gamma-tocopherol and alpha-tocopherol. 2. By using [(14)C]-homogentisic acid it has been shown that homogentisic acid is also a precursor of plastoquinone, tocopherols and alpha-tocopherolquinone in the higher plants Lactuca sativa and Rumex sanguineus, the green algae Chlorella pyrenoidosa and Euglena gracilis and the blue-green alga Anacystis nidulans.


Properties and reaction with iodoacetamide of adenosine 5'-triphosphate-creatine phosphotransferase from human skeletal muscle. Further evidence about the role of the essential thiol group in relation to the mechanism of action. 1. The purification of creatine kinase from human and monkey skeletal muscle by horizontal electrophoresis on Sephadex blocks is described. 2. The purified enzymes are shown to have similar chemical and kinetic properties to the rabbit muscle enzyme and a common mechanism is inferred. 3. Iodoacetamide has a similar apparent second-order inhibition constant with the human and rabbit enzymes, but the inhibition does not go to completion with the former. This is even more marked with the monkey enzyme, which has more reactive thiol groups, but inhibition is only about 50%. 4. Single substrates have little effect on the inhibition by iodoacetamide, but with the primate enzymes, in contrast with the rabbit enzyme, high concentrations of ADP-Mg(2+) plus creatine convert the essential thiol group from being pH-independent into one with a normal ionization. Low concentrations of ADP-Mg(2+) plus creatine first enhance the rate of inactivation, but cause protection as the reaction proceeds. These results are interpreted to indicate an activation of the thiol group on the subunit to which the substrates bind and a co-operatively induced decrease in the activity of the thiol group on the other subunit which lacks substrates. 5. The effects of a substrate equilibrium mixture on the rate of inhibition are essentially those of ADP-Mg(2+) plus creatine. 6. Since no substrate combination affords significant protection to the thiol group associated with the catalytic site to which the substrates are bound, it is concluded that any mechanism involving the thiol group in a direct participation in the transition-state complex of the catalytic reaction must be abandoned unless the transition state is only a small part of the time taken for one catalytic cycle.


Pyocyanine degradation by apyocyanogenic strains of Pseudomonas aeruginosa.


The marginal plasma zone in the retinal microcirculation.


Organization and development of the macronuclear anlage in Stylonychia notophora Stokes.


Morphogenesis of a large microtubular organelle and its association with basal bodies in the ciliate Nassula.


Identification of species origin of tissues found in a sewer.


A study of serum protein changes in late pregnancy and identification of the pregnancy zone protein using antigen antibody crossed immunoelectrophoresis.


A management of the third stage to reduce feto-maternal transfusion.


Transplacental haemorrhage due to termination of pregnancy.


Feto-maternal haemorrhage at therapeutic abortion.


Bacteriology of fallopian tube in relation to puerperal sterilization.


Liquor bilirubin values in early pregnancy.


Teenage pregnancy--an intr-racial study.


Characterization of infectious deoxyribonucleic acid from temperature Bacillus subtilis bacteriophage phi105. Phenol-extracted, infectious deoxyribonucleic acid (DNA) species from phi105 phage particles, from phi105 lysogenic bacteria, and from induced phi105 lysogenic bacteria were sedimented in sucrose gradients. Infectious DNA from phi105 particles sedimented like the bulk of mature phage DNA in neutral sucrose. Infectivity of prophage DNA was associated with fast-sedimenting material of heterogenous size. Infectious vegetative phage DNA sedimented somewhat faster than mature phage DNA; it was rapidly converted to a poorly infectious form during the infection.


Arenoviruses: proposed name for a newly defined virus group. The name "arenoviruses" is proposed for the newly defined taxonomic group containing lymphocytic choriomeningitis, Lassa, and the Tacaribe complex viruses.


Carrier cultures of simian foamy virus.

Carrier cultures of simian foamy virus. The production of cultures of HEp-2 and BHK-21 cells persistently infected with a type 1 simian foamy virus is described. After infection, HEp-2 cells showed no structural changes, whereas BHK-21 cells lost their normal spindle shape and showed mitochondrial damage, and some cells contained many lysosomes. Thin sections also showed that a few BHK-21 cells contained virus particles in low concentration, and infectious virus could be isolated from both the cells and the supernatant fluid. No virus was seen in thin sections of HEp-2 cells, although infectious virus in low titer could be recovered intermittently from lysed cells. Both carrier cultures were immune to challenge with homologous virus and antigen could be detected in over 90% of the cells even after growth for 9 weeks in the presence of virus-neutralizing serum. The distribution of antigen in carrier cultures of both cell types is described and compared with that seen in cytocidal infections.


Septic embolic actinobacillosis. A report of 2 cases in New World monkeys.


Surgical implictions of gastric diverticula.


WHO Expert Committee on Biological Standardization. Twenty-second report.


Exogenous reinfection in tuberculosis.

Exogenous reinfection in tuberculosis.


L forms in patients with cystic fibrosis.


Metabolism of D-glucose by Mycobacterium tuberculosis.


Infectiousness of Battey and Kansasii bacilli. Two levels of pathogenicity of Mycobacterium Kansasii strains.


Urinary infection in two selected neonatal populations.


Effects of isoniazid on mycolic acid synthesis in Mycobacterium tuberculosis and on its cell envelope.


Coproporphyrinogenase in tobacco (Nicotiana tabacum L.). 1. Coproporphyrinogenase was extracted and purified from tobacco (Nicotiana tabacum L.). Enzyme activity was mainly located in mitochondria rather than in chloroplasts. The enzyme was purified by differential centrifugation, ammonium sulphate fractionation, calcium phosphate gel adsorption and dialysis. A 69-fold final purification was obtained. 2. An apparent K(m) value of 3.6x10(-5)m was found, the value being largely dependent on the amount of coproporphyrin III recovered after reduction with sodium amalgam to coproporphyrinogen III. Protoporphyrin formation was linear up to 3h and decreased with further incubation. The enzyme activity increased with the concentration of enzyme protein up to 30mug/ml of solution. 3. Enzyme activity was greatly enhanced by increasing Fe(2+) concentrations up to 0.5mm, beyond which inhibition occurred. Co(2+) and Mn(2+) were also found to activate at low concentrations (0.1mm) and inhibit at higher concentrations (5mm). Fe(3+) and Cu(2+), both at 0.1mm, and o-phenanthroline and EDTA, each at 1mm, were found to be inhibitory.


Threonine metabolism in a strain of Bacillus subtilis.


The effect of trypsin or ethylenediaminetetraacetate on the surface of cells in tissue culture.


Studies on the competence-inducing factor of Bacillus subtilis. 1. Aqueous extracts of competent cells of Bacillus subtilis 168I(-) were shown to contain a competence-inducing factor. The aqueous extracts were fractionated on DEAE-cellulose columns. 2. Those fractions from DEAE-cellulose columns containing the competence-inducing factor were shown to exhibit a powerful lytic effect on isolated cell walls of B. subtilis 168I(-). Little or no lytic activity was exhibited by the other fractions. 3. The kinetics of the lytic enzyme were investigated and found to be first-order. Treatment of cell wall lysates with 1-fluoro-2,4-dinitrobenzene suggested that the enzyme may be identical with N-acetylmuramyl-l-alanine amidase. The amino acid composition of the partially purified enzyme was determined. 4. It is suggested that competence-induction may be dependent on the limited action of the autolytic amidase.


Inhibition of alcohol dehydrogenase by aminophenoxyalkanes: a possible mechanism of their retinotoxicity.


Thyrotrophin-releasing hormone.

Thyrotrophin-releasing hormone.


Mechanism of prophylaxis by silver compounds against infection of burns. To clarify tthe mechanism by which local application of silver compounds protects burns against infection, an ion-specific electrode was used to measùre the concentration of silver ions in solutions. By this method it was shown that in burn dressings silver ions were reduced to a very low level by precipitation as silver chloride. The antibacterial effect was found to depend on the availability of silver ions from solution in contact with precipitate. Between 10(-5) and 10(-6) molar silver nitrate solution in water was rapidly bactericidal. The minimal amount of silver nitrate causing inhibition of respiration of skin in tissue culture was about 25 times the minimal concentration of silver nitrate that inhibited growth of Pseudomonas aeruginosa.


Thyroid-stimulating hormone response to synthetic thyrotrophin releasing hormone in man. Synthetic thyrotrophin-releasing hormone, L-pyroglutamyl-L-histidyl-L-proline-amide, has been administered intravenously to euthyroid subjects in doses ranging from 1 to 200 mug. Its effect was assessed by serial measurements of serum thyroid-stimulating hormone (T.S.H.). A rise in serum T.S.H. was detectable five minutes after administration of 50 mug. of thyrotrophin-releasing hormone, reaching a maximum in 15 to 30 minutes, and then gradually declining over the next 120 minutes. Doses of 100 and 200 mug. of thyrotrophin-releasing hormone produced even greater rises of T.S.H. in some cases. The only side-effect noted was transient nausea with doses greater than 50 mug. Measurement of serum T.S.H. levels after administration of thyrotrophin-releasing hormone should prove a useful test of pituitary T.S.H. release and may help to distinguish pituitary and hypothalamic lesions.


The radiology of tuberculosis of the urinary tract.


Polyps in the large bowel in children.

Polyps in the large bowel in children.


The antigens of Vibrio cholerae involved in the vibriocidal action of antibody and complement.


Transcription of the tryptophan operon in Escherichia coli: rifampicin as an inhibitor of initiation.


Ciliate ultrastructure: some problems in cell biology.


Tissue-air ratios for rotational therapy with 32-MeV x rays.


Dosimetry of fast neutron beams from a small medical cyclotron.


Oophoropexy: a means of preserving ovarian function following pelvic megavoltage radiotherapy for Hodgkin's disease.


Dynamic equilibrium in squirrel monkeys after unilateral and bilateral labyrinthectomy.


Further studies on thrombin-coagulase.

Further studies on thrombin-coagulase.


Comparative dissolution studies on human enamel and fluorapatite.


A levansucrase from Streptococcus mutans.


Bacteriophage production by autotrophically growing Hydrogenomonas facilis.


Characteristics of bacteriophages attacking psychrophilic and mesophilic pseudomonads. Ten Pseudomonas phage were isolated by sewage enrichment. Five psychrophilic and five mesophilic phage were selected for a description of some of their biological properties. In addition to growth on psychrophilic hosts, the psychrophilic phage studied were also able to grow on a mesophilic host within its growth temperature range. Latent periods for psychrophilic phage at 3.5 C were 6 to 12 hr and at 25 C were 30 to 60 min. Mesophilic phage had a latent period of 85 to 190 min at 25 C and 35 to 85 min at 37 C. Psychrophilic phage were significantly more heat-sensitive than the mesophilic phage. Of all the parameters studied, only thermal sensitivity correlated with growth at 3.5 C. Phage used in this study had a deoxyribonucleic acid base composition ranging from 39.6 to 68.2% guanine plus cytosine, deduced from melting temperature measurements.


Mechanism of enhancement of virus plaques by cationic polymers. It has been assumed that plaque enhancement by cationic polymers is due to their binding of sulfated polysaccharides in agar. However, viruses that are enhanced by cationic polymers, diethylaminoethyl-dextran, and protamine were found not to be inhibited by polyanions in agar under the usual overlay conditions. In the case of adenovirus, enhancement by protamine seems to be due to the protamine serving as a source of arginine; enzymes released from the cultured cells digest the protamine and provide a reservoir of arginine for the cells. Other viruses (herpes and echovirus types 3, 4, 5, and 6) known to be susceptible to agar inhibitors were found to be enhanced by cationic polymers even under starch gel and methylcellulose overlays, which are free of polyanions. Since cationic polymers enhance the diffusion of virus through agar or starch gel, plaque enhancement seems to be the result of the gel becoming positively charged so that viruses can move effectively through them. The observation that starch gel and methylcellulose enhance plaque formation with viruses known to be inhibited under agar was also reinvestigated. When the consistency of the agar gel was reduced to the same viscosity of starch gel and methylcellulose overlays, the same plaque counts and sizes were observed under all three overlays.


Ultrastructure of lymphocystis virus.

Ultrastructure of lymphocystis virus. Lymphocystis virus obtained from bluegills (Lepomis macrochirus) was cultured in the permanent bluegill cell line BF-2 and examined by electron microscopy in ultrathin sections of cell cultures and in negative-contrast preparations from cells and from centrifuged culture medium. According to negative-contrast preparations, the icosahedral virions have an overall diameter close to but not exceeding 300 mmu. Delicate filaments seem to issue from the vertices. In collapsed virions, an ordered array of morphological units was seen. Positively contrasted virions in ultrathin sections show a shell with three dark (heavy metal-stained) layers alternating with and separated by two clear layers. The acquisition of an additional outer membrane during release from the cell, as found in African swine fever virus, was never seen. Morphologically, lymphocystis virus is considered to be closely related to Tipula iridescent virus.


Physicochemical and biological properties of fibrous Pseudomonas bacteriophages. Two fibrous Pseudomonas phages, Pf1 and Pf2, have been isolated and characterized. The phages were serologically related and were indistinguishable morphologically when viewed by electron microscopy. Both phages formed minute turbid plaques. A simple method was devised for easy differentiation of such plaques from the bacterial lawn. The infected bacteria continued to grow and to liberate the phages into the medium. The phages were collected from the turbid "lysate" by salting out and were purified by differential centrifugation and density gradient centrifugation. The purified phages were sensitive to ultrasonics and to a proteolytic enzyme, Nagarse. A rapid lysis mutant was obtained from the progeny of plaquepurified Pf2. Pf2 was spontaneously produced by strain P28 of P. aeruginosa. Neither acridine orange nor antiserum affected the phage-producing capacity of the bacteria.


Nucleotide phosphohydrolase in purified vaccinia virus. Purified infectious vaccinia virus has been shown to contain an enzyme or enzymes that remove the terminal phosphate group from adenosine triphosphate (ATP), guanosine triphosphate (GTP), uridine triphosphate (UTP), and cytidine triphosphate (CTP). The K(m) for ATP of this enzyme is 5.5 x 10(-4)m, and the relative rates of the reaction with ATP, GTP, UTP, and CTP are 1.00, 0.34, 0.15, and 0.29, respectively. The virus enzyme does not react with any of the diphosphates. The rate of the reaction is proportional to the amount of virus added and is linear for 130 min. The virus nucleotide phosphohydrolase activity is greatly stimulated by Mg(++) and very slightly stimulated by Ca(++). The small residual activity observed in the absence of divalent cations is completely inhibited by ethylenediaminetetraacetic acid. Neither Na(+) nor K(+) ions, nor any mixture of these, was found to stimulate the reaction significantly, and ouabain, at 10(-4)m, inhibited the reaction by only 27%. The response of the vaccinia enzyme to mono- and divalent cations and to ouabain indicates that the vaccinia enzyme has different properties from those associated with microsomes and mitochondria.


Primary adsorption site of phage PBS1: the flagellum of Bacillus subtilis. The adsorption of Bacillus subtilis phage PBS1 was studied, and it was demonstrated that the primary adsorption site for this phage is the flagellum of B. subtilis. The capacity of flagella to function for motility may be lost without the loss of their capacity to adsorb the phage and permit infection. Deoxyribonucleic acid injection by the phage is inhibited by cyanide, suggesting the requirement for cellular energy in the infection process.


Diabetic ketoacidosis.

Diabetic ketoacidosis.


Delayed fibrin polymerization due to removal of calcium ions.


Immunochemical identification of specific precipitin lines in quantitative immunoelectrophoresis patterns.


The meibomian glands of the marmoset (Callithrix jacchus).


Coarctation of the aorta and other late sequelae of administration of thorium dioxide (thorotrast).


The influence of adjuvants on the immunological response of the chicken. I. Effects on primary and secondary responses of various adjuvants in the primary stimulus.


The influence of adjuvants on the immunological response of the chicken. II. Effects of Freund's complete adjuvant on later antibody production after a single injection of immunogen.


Survey on the vitamin aspects of coenzyme Q.


Long-term dietary management of dialysis patients. I. Pathologic problems and dietary requirements.


C-type virus and lymphocytic nuclear projections in bovine lymphosarcoma.


Vitamin B12 levels in erythrocytes in normal subjects and in pernicious anaemia. A reliable method is described for the assay of vitamin B(12) activity in erythrocytes using Lactobacillus leichmannii. The results are shown for age, sex, and blood group in normal subjects, and before, during, and after therapy in vitamin B(12) deficiency anaemia. The normal range is 85-225 pg/ml (mean = 155 +/- 35 pg/ml). Raised levels were found in the newborn, and subnormal levels in subjects aged 70 years and over and in males aged 10 to 19 years. There were no differences in erythrocyte vitamin B(12) levels between the ABO or Rhesus blood groups. In pernicious anaemia low levels are found before treatment (mean = 91 pg/ml), followed by a rapid rise during vitamin B(12) therapy (mean peak = 482 pg/ml), and falling more slowly towards normal after the initial haematological response.


Nursing in long term settings.

Nursing in long term settings.


Affinity for glycoproteins of bacteria found in the respiratory tract in cystic fibrosis. The attraction of bacteria commonly found in the respiratory tract in cystic fibrosis to glycoprotein at pH 7 has been studied. The effect of washing on the removal of bacteria from the glycoprotein has also been investigated, and the value of doing both washed and unwashed sputum cultures is discussed.


Gram-negative bacillary necrotizing pneumonia: a bacteriologic and histopathologic correlation.


Bacterial interference. I. Its occurrence among the respiratory tract flora and characterization of inhibition of group A streptococci by viridans streptococci.


A preliminary report on the experimental host range of the transmissible mink encephalopathy agent.


Serum protein changes in the parturient mother, fetus and newborn infant.


Occurrence of systemic lupus erythematosus in association with ethosuccimide therapy. Case report.


Two common EEG patterns of doubtful clinical significance.


The electroencephalogram in the evaluation of seizures. Current clinical applications.


Schistosomal pigments in murine hepatic bilharziasis.


Microspectrophotometric studies of DNA on the epithelial tumors induced by 3 methylcholanthrene and croton oil treatment in mice.


Studies on Crocker sarcoma under the effect of alkylating agents. II. Cytochemistry of nucleic acids and proteins in malignant cells of sarcoma 180 after treatment with the antitumour drug DL-sarcolysine.


A microspectrophotometric method for histochemical quantitation of succinic dehydrogenase.


Histochemical studies on the effect of some anaesthetics on rat liver.


Localization of acid and alkaline phosphatase activities in the duodenum of the chick.


Efferent projections of the ventral anterior nucleus of the thalamus in the monkey.


Pulmonary parenchymal ossific nodules in idiopathic hypertrophic subaortic stenosis.


Fetal and maternal placental circulations. Simultaneous visualization in monkeys by radiography.


Purification and properties of alpha-D-mannosidase from the limpet, Patella vulgata. 1. alpha-Mannosidase from the limpet, Patella vulgata, was purified nearly 150-fold, with 40% recovery. beta-N-Acetylglucosaminidase was removed from the preparation by treatment with ethanol. The final product was virtually free from beta-galactosidase. 2. Limpet alpha-mannosidase was assayed at pH3.5 and at this pH it was necessary to add Zn(2+) for full activity. At pH5, the enzyme had the same activity in the presence or absence of added Zn(2+). 3. On incubation at acid pH, the enzyme underwent reversible inactivation, which was prevented by adding Zn(2+). 4. EDTA accelerated inactivation and the addition of Zn(2+) at once restored activity. No other cation was found to reactivate the enzyme. 5. Cl(-) had an unspecific effect on hydrolysis by limpet alpha-mannosidase. It increased the rate of reaction with substrate. The anion did not prevent or reverse inactivation by EDTA. 6. It is concluded that alpha-mannosidase is a metalloenzyme or enzyme-metal ion complex, dissociable at the pH of activity, and that it requires Zn(2+) specifically.


Protein-hydrocarbon interactions. Interactions of various proteins with pure decane. 1. Solutions of a number of proteins were subjected to gentle agitation in the presence of small quantities of decane. 2. Some of the protein was lost from solution and adsorbed on the surface of the emulsion formed; at the same time some of the decane was bound to the protein remaining in solution. 3. The two processes were found to be related and a mechanism is proposed to explain the relationship. 4. With lysozyme and ribonuclease, the protein in the aqueous phase of an emulsion exhibited normal enzymic activity, whereas the fraction adsorbed on the interface was much less active but recovered activity on desorption.


A reinvestigation of the substrate specificity of pig kidney diamine oxidase. 1. The substrate specificity of pig kidney diamine oxidase was reinvestigated with a purer enzyme preparation than has previously been used for this purpose. 2. All substrates were extensively purified before use, and methods of preparation or sources are given, together with R(F) values. 3. The substrate specificity determined differed somewhat from that reported by previous workers and, in addition, the behaviour of several compounds not previously used as substrates is described. 4. A model for enzyme-substrate interaction embodying these observations is formulated. It is suggested that a negatively charged substrate-binding group is situated at 6.0-9.0 A from the oxidizing site. The binding and oxidizing sites are separated by a hydrophobic or methylene-binding site.


The acute toxicity of retrorsine, aflatoxin and sterigmatocystin in vervet monkeys.


The effect of anti-D IgG on D-positive recipients. When one standard prophylactic dose of anti-Rh(D) immunoglobulin (Connaught) (and in one case two doses) was injected into 15 D-positive volunteers it caused no reaction and no measurable alteration in readings of hemoglobin, hematocrit, reticulocytes or bilirubin, although some of the volunteers briefly became weakly direct Coombs'-positive. It is argued that since any isohemagglutinin that may contaminate anti-D IgG must be much less in amount than the anti-D it contains, the contaminating antibody cannot carry a greater potential for harm to a recipient having the corresponding antigen than the anti-D for a D-positive recipient, which is zero. It is suggested that the presence of anti-DC or G in so-called anti-Rh(D) IgG may, in fact, be a mark of excellence. The application of these facts to selection of plasma donors and to tests required before giving prophylactic treatment to women is briefly stated.


Fracture dislocation of the ankle.

Fracture dislocation of the ankle.


Experimental studies of sodium pyridinethione. I. Percutaneous absorption in laboratory animals.


Experimental studies of sodium pyridinethione. II. Urinary excretion following topical application to rats and monkeys.


Character of non-precipitating antibodies.


Catabolism of gamma G-globulin and myeloma proteins of the subclasses gamma G1 and gamma G2 in a healthy volunteer.


Studies of allograft immunity in mice. I. Induction, development and in vitro assay of cellular immunity.


Serological changes in immunoglobulins IgG, IgA and IgM and Escherichia coli antibodies in the young pig.


Plasmodium vivax infections in the spider monkeys, Ateles fusciceps and A. geoffroyi.


Effects of triaminoquinazolines against Trypanosoma cruzi in mice.


Specific gravity measurements on baboon spermatozoa.


Parvovirus infections, vascular endothelium, and hemorrhagic encephalopathy.


The effects of d-amphetamine on schedule-controlled water licking in the squirrel monkey.


Limitations in the quantitative application of microslide diffusion analyses.


The role of serum hepatitis virus in chronic liver disease.


Comparative concentrations of selected trace metals in human and marmoset saliva.


Immunological studies of the A and B chains of insulin.


Immunoglobulins and antibody activity in the intestine and serum in cholera. I. Analysis of immunoglobulins in cholera stool.


Production of highly purified choleragen and choleragenoid.


Purification and properties of permeability factor-cholera enterotoxin from complex and synthetic media.


Comparison of the effects of an antibacterial leukocytic extract and the serum endotoxin-detoxifying component of lipopolysaccharides extracted from rough and smooth Salmonellae.


Enhanced proliferation of H-1 virus in livers of rats infected with Cysticercus fasciolaris.


Apparent new modes of antimalarial action detected by inhibited incorporation of adenosine-8-3H into nucleic acids of Plasmodium berghei.


Comments on host-parasite-drug interactions.


Quantitative cytochemistry of malaria infected erythrocytes (Plasmodium berghei, Plasmodium chabaudi and Plasmodium vinckei).


Amino acid activation for protein synthesis in Plasmodium berghei.


Biogenesis of ribosomes in Plasmodium berghei.


Malarial parasite metabolism: the metabolism of methionine by Plasmodium berghei.


Interaction of red cell ATP levels and malaria, and the treatment of malaria with hyperoxia.


Lipid synthesis in vivo from 1-14C-oleic acid and 6-3H-glucose by intraerythrocytic Plasmodium berghei.


Analyses on the blood of normal monkeys and owl monkeys infected with Plasmodium falciparum.


Comments on parasite molecular biology.

Comments on parasite molecular biology.


Anemia in rats with equivalent splenomegalies induced by methyl cellulose and Plasmodium berghei.


Alterations of red blood cell metabolism in simian malaria: evidence for abnormalities of nonparasitized cells.


Influence of chloroquine and phenanthrene methanols (WR 33,063) on content of biogenic amines in the mouse lung.


Comments on pathophysiology.

Comments on pathophysiology.


Glomerulonephritis associated with acute hemosporidian infection.


Plasmodium falciparum in the white-handed gibbon: protection afforded by previous infection with homologous and heterologous strains obtained in Thailand.


Characterization of protective antibodies produced in Plasmodium berghei infected rats.


Cell-mediated immunity in rats infected with Plasmodium berghei.


Aspects of immunity in mice inoculated with irradiated Plasmodium berghei.


Resistance produced in owl monkeys (Aotus trivirgatus) by inoculation with irradiated Plasmodium falciparum.


Protective immunity produced by the injection of x-irradiated sporozoites of Plasmodium berghei. IV. Dose response, specificity and humoral immunity.


Protective immunity produced by the injection of x-irradiated sporozoites of Plasmodium berghei. V. In vitro effects of immune serum on sporozoites.


Comments on immunization.

Comments on immunization.


Mechanisms of erythrocyte destruction in chickens infected with Plasmodium gallinaceum.


Autoimmune reactions in chickens with Plasmodium gallinaceum infection: the isolation and characterization of a lipid from trypsinized erythrocytes which reacts with serum from acutely infected chickens.


Immunopathology of renal complications in simian malaria and human quartan malaria.


Comments on immunopathology.

Comments on immunopathology.


Some characteristics of Plasmodium berghei "relapsing" in immunized mice.


Partial protection and precipitin passively transferred to their litters by mother rats infected or superinfected with Plasmodium berghei.


The effect of antisera to normal rat and mouse erythrocytes on the infectivity of Plasmodium berghei.


The immunogenic activities of the non-specific serum antigens of acute Hemosporidian infections.


Resistance of ducks to a Plasmodium sp. induced by a filterable agent.


An indirect hemagglutination test for malaria using an antigen from the lysate of parasitized erythrocytes.


Malarial antibodies in human volunteers. A comparison of the soluble antigen fluorescent antibody (SAFA) and the indirect hemagglutination (IHA) tests using as antigen Plasmodium falciparum parasitized erythrocyte lysates.


Comments on serology.

Comments on serology.


Experimental malaria--recapitulation and concluding remarks.


The use of subhuman primates for experimental studies of human malaria.


Further studies on the biology of human malarial parasites in gibbons from Thailand.


Experimental infections of Plasmodium falciparum in Cebus capucinus (white faced capuchin) monkeys.


Susceptibility of mosquitoes to human malaria induced in Panamanian monkeys.


Biological basis for susceptibility of Aotus trivirgatus to species of plasmodia from man.


Effects of quinine and of chloroquine in vitro against a strain of chloroquine-resistant Plasmodium falciparum that displays a relative resistance to quinine in vivo.


Characteristics of four new drug-resistant strains of Plasmodium falciparum from South-East Asia.


Gametocytocidal and sporontocidal effects of primaquine upon two strains of Plasmodium falciparum.


Comments on human Plasmodia.

Comments on human Plasmodia.


New insights into the fine structure of rodent malarial parasites.


The isolation and fractionation of Plasmodium knowlesi.


The identity of chloroquine induced nucleolar aggregates in malaria.


A protozoan virus.

A protozoan virus.


Comments on non-human Plasmodia.

Comments on non-human Plasmodia.


Nutritional requirements for in vitro cultivation of a simian malarial parasite, Plasmodium knowlesi.


In vitro studies on the amino acid metabolism of Plasmodium knowlesi and the antiplasmodial effect of the isoleucine antagonists.


Growth of Plasmodium berghei in parallel perfused blood cell suspensions.


Studies on the red cell-parasite relationship.


Incorporation of 14C-amino acids by malaria (Plasmodium lophurae). I. Role of ions and amino acids in the medium.


Comments on cultivation of Plasmodia.

Comments on cultivation of Plasmodia.


Comments on "studies on the red cell parasite relationship".


A tissue culture system for the study of drug action against the tissue phase of malaria.


Morphological effects of 8-aminoquinolines on the exoerythrocytic stages of Plasmodium fallax.


Parasitology of malaria. Report of a WHO Scientific Group.


The effect on the nitrogen balance of various amino acids infused intravenously.


Food requirements in normal children.

Food requirements in normal children.


Plate assay methods for amino acids. 10. Arginine and histidine.


Plate assay methods for amino acids. 11. Further studies on lysine.


Pseudofluorescence--a problem in interpretation of fluorescein angiograms.


Effect of echo 9 virus infection on tuberculin sensitivity.


The pattern of inheritance of alpha 1 antitrypsin deficiency and associated pulmonary disease.


Cellular basis of cortisone-induced host susceptibility to tuberculosis.


A comparative study of the virulence of mycobacterium tuberculosis measured in mice and guinea pigs.


Studies on the allergenicity of various tuberculoprotein derivatives and the adjuvanticity of wax D fractions of Mycobacterium tuberculosis.


Comparative study of the paper strip technique with the micro-spot plate and liquid medium methods for niacin.


Three lipoidal tests in screening for syphilis and biological false-positive reactions in a dermatological series.


Serological tests for syphilis in the elderly.


Acrylamide gel electrophoresis studies of human erythrocyte membrane.


Chemical derivatives of subtilisins with modified proteolytic activities. II. Succinyl- and glutarylsubtilisin type Carlsberg.


Pyelonephritis. IX. Effect of enterococcal pyelonephritis on mortality in the rat.


Increase in PBI caused by LT3.

Increase in PBI caused by LT3.


The nature of the scrapie agent. A review of recent progress.


The oxidative degradation of benzoate and catechol by Klebsiella aerogenes (Aerobacter aerogenes).


Characterization of the central body of the Azotobacter cyst.


Somatosensory thresholds--contrasting effects of postcentral-gyrus and posterior parietal-lobe excisions.


Activity and specificity of synthetic thyrotropin-releasing hormone in man.


Catalysis of formation of mixed disulfides between cystine and beta-globulins by copper ions.


The enzymic synthesis of ganglioside. II. UDP-galactose: N-acetylgalactosaminyl-(N-acetylneuraminyl)galactosyl-glucosyl-ceramide galactosyltransferase in rat brain.


Purification and partial characterization of the branched chain amino acid transaminase of Pseudomonas aeruginosa.


Immobilization antigen synthesis in Paramecium aurelia synthesis in a cell-free amino acid incorporating system.


Ribonuclease activity in the reticulocyte cell-free system.


On the evolution of endopeptidases. 8. Cross-reactions of trypsins and chymotrypsins of different species.


Chromosome studies in pregnant rhesus macaque given LSD-25.


Sustained sensory input and epileptiform activity.


Urinary chorionic gonadotropin excretion in the monkey (Macaca mulatta)--early phase.


Phosphorylase a activity in rat uterine homogenates: protection by ethylenediaminetetraacetate.


Post-traumatic epilepsy 15 years later.

Post-traumatic epilepsy 15 years later.


A longitudinal electro-clinical study of 80 cases of post-traumatic epilepsy observed from the time of the original trauma.


Post-traumatic epilepsy in children.

Post-traumatic epilepsy in children.


Criteria for identifying cingulate epilepsies.


Post-traumatic epilepsies of multiple cortical origin.


Post-traumatic epilepsy. Introduction and clinical observations.


Physiopathological mechanisms of post-traumatic epilepsy.


The pathology of post-traumatic epilepsies.


General physiology of cerebral circulation.


Physiology of the cerebrospinal fluid.

Physiology of the cerebrospinal fluid.


Neurogenic control of the cerebral circulation.


Influence of cerebrospinal fluid pH on the regulation of cerebral circulation.


The distribution kinetics of triiodothyronine: studies of euthyroid subjects with decreased plasma thyroxine-binding globulin and patients with Graves' disease. The kinetics of distribution of 3,3',5-triiodo-L-thyronine (T(3)) have been studied employing both a single-injection and a continuous infusion of T(3-) (131)I. External monitoring of radioactivity in the liver during the infusion permitted estimation of the hepatic distribution volume (V(H)) and the one-way hepatic clearance (C(H)) of the hormone. Among 10 euthyroid control subjects, V(H) averaged 2.07 liters +/-0.50 (SD), and the mean value for C(H), 231 ml of plasma per min (+/-64). In three euthyroid men whose plasma showed decreased binding capacity by thyroxine-binding globulin (TBG) abnormally high V(H) and C(H) values were found, the increase in C(H) being proportional to the decrease in binding activity by plasma proteins. Among all 13 subjects, there was a high correlation (+ 0.86) between C(H) and the proportion of free hormone in plasma, measured in vitro. In four patients with hyperthyroid Graves' disease V(H) ranged from 3.2 to 4.2 liters and C(H) was elevated in every case, averaging 989 ml/min. The increase in C(H) in this group was out of proportion to the elevation of free hormone fraction in plasma. Seven patients who were either euthyroid or hypothyroid after treatment of Graves' disease showed a slight but significant increase in C(H) compared with the euthyroid controls without Graves' disease. The percentage of free hormone in the plasma of the treated group was normal or low and therefore could not explain the persistent elevation in unidirectional hepatic clearance observed. The rate of accumulation of labeled T(3) in the tissues of the thigh during the interval from 10 to 60 min of the sustaining infusion of tracer was slow compared to the rate of equilibration in the liver and did not differ significantly among the various groups studied. These latter findings suggest that in slowly equilibrating tissues such as the thigh the kinetics of T(3) distribution are relatively insensitive to alterations in hormone-binding activity by plasma proteins.


Gamma G globulin subgroup composition of the glomerular deposits in human renal diseases. 36 renal biopsies from patients with nephritis were studied for glomerular localization of the heavy chain subgroups of immunoglobulin G (IgG or gammaG). The deposition pattern of these subgroups was selective and did not reflect the normal serum concentration of these proteins. gammaG2, which comprises 18% of normal serum gammaG, was the predominant or unique subgroup deposited in five cases of lupus nephritis and four biopsies with other forms of nephritis associated with granular gammaG deposits. gammaG3, which normally makes up only 8% of the serum gammaG, was the dominant subgroup seen in one biopsy of lobular glomerulonephritis. Patients with linear gammaG deposits generally had a selective absence of gammaG3 and often had large amounts of gammaG4 (normally 3% of the serum gammaG) deposited. The deposition of complement components C1q, C4, and C3 was variable. One biopsy had only gammaG2 and no complement components in the deposits and had no neutrophile leukocyte infiltration. This latter observation correlates well with the poor ability of gammaG2 to fix complement in vitro. Similarly, deposits containing large amounts of gammaG4, which does not fix complement, also tended to have less inflammatory infiltrate than deposits devoid of this subgroup. The selective deposition of monotypic or restricted gammaG subgroups on the glomerulus supports the likelihood that the gammaG represents antibody. The nature of the subgroup involved in the deposit may represent one variable in the determination of the inflammatory and morphological picture that evolves in human glomerulonephritis.


Immunoglobulin synthesis and total body tumor cell number in IgG multiple myeloma. Studies of synthesis of IgG paraproteins were performed in 10 patients who had IgG myeloma in order to quantitate cellular immunosynthetic functions and derive estimates of the number of tumor cells present in such patients. Serial in vitro studies demonstrated constancy in the cellular rate of IgG paraprotein secretion for up to 8 months. Average molecular synthesis rates in different patients ranged from 12,500 to 85,000 molecules of IgG per minute per myeloma cell. Estimated total body tumor cell number ranged from 0.5 x 10(12) to 3.1 x 10(12) myeloma cells, and could be correlated with the degree of skeletal damage observed on roentgenograms (P = <0.01). Serial measurements of tumor cell number may prove useful in characterizing the growth rate and natural history of multiple myeloma. Myeloma is the first metastatic human malignancy in which quantitative measurements of the body's burden of malignant cells have been obtained.


Chronology of viral functions in bacteriophage alpha. Temperature-sensitive mutants of phage alpha were subjected to short pulses of permissive temperature at various times during the lytic cycle. All the mutants showed an optimal response to the permissive pulse at a specific time after infection. The optimal responses of the mutants belonging to the same complementation group fell close together in the same time interval; the optimal responses of mutants contained in 20 different complementation groups were more or less uniformly scattered throughout the lytic cycle. Temperature sensitivity, therefore, seems to afford, at least in the case of phage alpha, an independent way of grouping the genes in an ordered sequence with respect to the steps they control.


The effect of diphenylhydantoin on thyroxine metabolism in man. The effect of 5,5'-diphenylhydantoin on thyroxine metabolism was examined in five normal volunteers. Intravenous injection of radiothyroxine was followed by a 10-12 day control and subsequent 9-14 day treatment periods. During oral administration of diphenylhydantoin, plasma thyroxine concentration decreased to about 80% of its pretreatment level and the plasma radiothyroxine disappearance rate increased a maximum of 20% over control estimates. These changes were a result of increases in both urinary and fecal excretion of radioisotope.A minimum plasma thyroxine was apparent after 10-12 days of diphenylhydantoin administration. In two of the subjects, treatment was sufficiently prolonged to achieve this new steady state. In these subjects, the decrease in total body thyroxine was balanced by the increase in the fractional turnover rate. As a result, absolute thyroxine degradation during diphenylhydantoin administration was unchanged from the pretreatment values. Plasma ultrafiltration was used to estimate the free thyroxine fraction at regular intervals during the control and treatment periods. During diphenylhydantoin treatment, there was little or no change in this fraction and therefore, absolute free thyroxine decreased. Thyroxine-binding globulin and thyroxine-binding prealbumin capacities remained constant. These results indicate that thyroxine degradation can proceed at a normal rate in subjects receiving diphenylhydantoin despite decreases in plasma free thyroxine concentration. If free thyroxine is the only portion of the hormone available for cellular utilization, then free thyroxine clearance must be increased in these subjects. This increase in clearance could represent either a direct stimulation of peripheral thyroxine metabolism by diphenylhydantoin, or it could reflect the response of intrinsic regulatory systems to a diphenylhydantoin-mediated displacement of thyroxine from thyroxine-binding globulin. Whatever the mechanism for this effect, a decreased free thyroxine value in patients receiving diphenylhydantoin may not imply hypothyroidism.


Molecular weight of bacteriophage PBS 1 deoxyribonucleic acid.


Plaque assay for lymphocytic choriomeningitis virus based on hemadsorption interference.


Reproducible plaquing system for rabies, lymphocytic choriomeningitis,k and other ribonucleic acid viruses in BHK-21-13S agarose suspensions. BHK21/13S-agarose suspension cultures provide an excellent plaquing system for rabies and lymphocytic choriomeningitis viruses. The system has facilitated accurate and reproducible assay of serum neutralization and cloning of these viruses. Rubella, reo, and Kern Canyon viruses may also be assayed by this technique.


Neurological effects of systemic circulatory arrests in the monkey.


Physicochemical characterization of adeno-associated satellite virus type 4 and its nucleic acid. Based on protein, deoxyribonucleic acid (DNA), and phosphorus analyses, adeno-associated satellite virus type 4 has a DNA content of 26.5%, significantly larger than type 1 which contains 18.9% DNA. Type 4 DNA contains 58 to 62% guanine + cytosine, as calculated from thermal denaturation temperature and buoyant density measurements. Simian adenovirus SV15, which serves as a helper virus for type 4 satellite, contains 13.7% DNA with a guanine + cytosine content of 55 to 57%. The DNA extracted from type 4 satellite virus is a linear, double-stranded molecule, as shown by thermal denaturation temperature profile measurements and by electron microscopy. The contour length and sedimentation coefficient of type 4 DNA indicate a molecular weight of 3.0 x 10(6) daltons.


The acute pharmacological actions of daunomycin in the dog and monkey.


The effect of analgesics on the hexobarbital sleeping times of rats, dogs, and rhesus monkeys: a species difference.


Chronic toxicity of DMSO in primates.

Chronic toxicity of DMSO in primates.


Animal inhalation studies on ammonia, ethylene glycol, formaldehyde, dimethylamine, and ethanol.


Alteration of the electroencephalogram during bromotrifluoromethane exposure.


The toxicity and pharmacodynamics of EGTA: oral administration to rats and comparisons with EDTA.


Long-term inhalation screening studies of benzene, toluene, o-xylene, and cumene on experimental animals.


Determination of the soybean root-nodule origin by the Ouchterlony method.


Comparison of cephalexin, oxacillin, cloxacillin, dicloxacillin and nafcillin in streptococcal infections in monkeys.


Persistence of an Escherichia coli-Shigella flexneri hybrid in the intestinal tract of Macaca mulatta. A live oral vaccine prepared from an Escherichia coli-Shigella flexneri 4b hybrid was administered by gavage to Macaca mulatta. In the three main studies involving 160 monkeys, three doses were used, generally consisting of 5 x 10(9) to 60 x 10(9) cells per dose, with an interval between doses of 6, 7, or 14 days. Rectal swab cultures at the time of the last vaccine dose, and 14 to 58 days later, revealed that the hybrid persisted in the intestinal tract for at least 7 days in 16%, and for at least 21 days in 8%, of the monkeys. Our findings are comparable to those of Formal et al. for shedding of an E. coli-S. flexneri 2a hybrid. Protection studies with the E. coli-S. flexneri 4b hybrid are indicated.


Autologous anti-I and anti-M following liver transplant.


Dengue virus plaque development in simian cell systems. I. Factors influencing virus adsorption and variables in the agar overlay medium. Chemical and physical variables influencing the plaquing of all dengue serotypes in two simian cell systems were studied. Calf serum in the nutrient overlay may be replaced by mouse ascitic fluid or bovine plasma albumin when employing the rhesus monkey kidney LLC-MK(2) cell system for plaquing all dengue serotypes. Doubling the serum concentration in the overlay had little effect in modifying dengue types 1, 2, 3, and 4 plaque titers. Newborn agamma, 4-week-old and 8-week-old calf serum gave comparable titers with all dengue virus serotypes. Dengue virus titers, plaque size, and development time were unaffected by sodium bicarbonate concentrations ranging from 1.1 to 4.4 mg/ml of overlay. A twofold increase (0.00332 g%) in the amount of either autoclaved or filtered-sterilized neutral red reduced the dengue 2 virus titer as much as 2.2 logs. An increased Mg(++) and decreased Ca(++) concentration in the overlay medium increased the efficiency of the plaquing system.


Thermoelectrically cooled temperature-gradient apparatus for comparative cell and virus temperature studies. Establishment of a near-linear temperature gradient in an incubator has been accomplished by the application of heat to one terminus of a conducting body, normally a metal bar, and the removal of heat from the other terminus of the conducting body. Such incubators have been complex and unwieldy because of the need for mechanical refrigeration. We have described a simplified temperature gradient incubator which uses thermoelectric module cooling coupled with electric heating. Along the gradient, 20 stations in two parallel rows of 10, each accommodating a 30-ml plastic cell culture flask, were continually monitored by an electronic thermometer, and the temperatures were recorded. By manipulation of two simple potentiometer controls, any temperature gradient between 0 and 50 C could be obtained. Minor deviations which occurred between theoretically perfect and obtained temperature gradients were reproducible and readily measured. The gradient incubator was particularly applicable to (i) simultaneously studying a given biological activity over the entire temperature range supporting the growth of a given cell, virus, or microorganism, or (ii) precisely defining the upper or lower temperature limits of a biological system by 10-point determinations. Preliminary experiments have demonstrated the usefulness of the apparatus in characterizing the temperature limits for growth in vitro of cells of reptilian cell lines. The gradient incubator was also successfully utilized for the characterization of the effect of temperature on the efficiency of plating of amphibian viruses and possible temperature variants of those viruses.


Low antibiotic potency of isohumulone.

Low antibiotic potency of isohumulone. Isohumulone is 15 to 30 times less inhibitory than lupulone or humulone on the growth of gram-positive bacteria.


Serous otitis media. An experimental model.


The discharge characteristics of single units in the oculomotor and abducens nuclei of the unanesthetized monkey.


Studies of the production and subsequent reduction swelling in primate. Cerebral cortex under isosmotic conditions in vivo.


Bacteriuria and arterial hypertension in old age.


Exaggeration of self-aggression following alcohol ingestion in rhesus monkeys.


Epizootiology, diagnosis, and control of Brucella canis.


A review of viruses recovered from dogs.


Use of cell cultures in vaccine production: diploid and stable cell cultures.


New technique for investigating the histopathology of the lymphatic system in experimentally infected animals.


Immune and naturally occurring antibodies detected in a general hospital blood bank.


Subunit composition of B. subtilis RNA polymerase.


In vitro cytotoxic activity of thymus cells sensitized to alloantigens.


Conformational changes in the antigenic determinant of tobacco mosaic virus protein resulting from polymerization of the subunits.


Synthesis of proteins in cells infected with herpesvirus. V. Viral glycoproteins.


C-reactive protein in serum from nfants as determined with immunodiffusion techniques. I. Healthy infants.


Action of mutagenic chemicals on Anacystis nidulans. V. Diethyl sulphate.


Periodontics in general practice.

Periodontics in general practice.


Periodontics--prosthodontics, their interrelationship.


The effect of maleic hydrazide on growth and mutation of a blue-green alga.


School of Dental Hygiene. Director's report.


Editorial. Needed--a laboratory prescription book for Arkansas dentists?


Identification through synthesis of an active delta-1(6)-tetrahydrocannabinol metabolite.


The 1970 Alfred C. Fones Award.

The 1970 Alfred C. Fones Award.


The Hackett technique for constructing claspless partial dentures and bridges.


Editorials. A tribute to the great dental editors of the near past.


Editorial. Need for continuing education and library habits.


Editorial. Oral exfoliative cytology.

Editorial. Oral exfoliative cytology.


Dental continuing education in Connecticut.


Oral medicine. 21--Transplantation of neoplasms.


Juvenile myxedema.

Juvenile myxedema.


Drug toxicity.

Drug toxicity.


A yearning for learning.

A yearning for learning.


Gearing adult education for the seventies.


Landmark statement from the American Nurses' Association on inservice education.


Inservice education: is it really "in?

Inservice education: is it really "in?


Staff development for the professional in the nursing service department.


Introducing the Journal of Continuing Education and the editor.


Editorial: creation of The Journal of Continuing Education in Nursing.


Pulmonary fibrosis in pigeon breeder's disease.


Studies on the reconstitution of rat liver ribosomes from different types of core particles and split proteins.


Comparison of the suitability of Nekal BX and trisodium phosphate for homogenization of diagnostic specimens.


Thanatophoric dwarfism.

Thanatophoric dwarfism.


Studies on cat brain antigens. I. Soluble precipitation protein antigens.


Inhibition of coronary vasodilating action of dipyridamole and adenosine by aminophylline in the dog.


Serum alpha-feto-protein and primary cancer of the liver in man.


Hypothalamic control of the distribution of cardiac output in the unanesthetized rhesus monkey.


The effect of porcine thyrotropin (TSH)-releasing hormone (TRH) on the release of TSH from sheep and goat anterior pituitary tissue in vitro.


The brain, seat of emotions.

The brain, seat of emotions.


A technique for treating significant gastrostomy leakage in infants and children.


Congenital hemihypertrophy of the face: impersonator of childhood facial tumors.


Determination of phenoxymethyl penicilloic acid and phenoxyethyl penicilloic acid in urine in the presence of the parent penicillins.


Gas chromatographic determination of N-gamma-phenylpropyl-N-benzyloxy acetamide (W-1372) in blood.


Intravenous hyperalimentation.

Intravenous hyperalimentation.


Hyperosmolar hyperglycemic nonketotic diabetic coma.


Pathology of the kidney in Waldenström's macroglobulinemia. Study of sixteen cases.


The kidney in macroglobulinemia.

The kidney in macroglobulinemia.


Man: sapient but gouty.

Man: sapient but gouty.


Total intravenous feeding.

Total intravenous feeding.


Serum alpha-feto-protein. IV. Effects of chemotherapy and radiotherapy on serum alpha-feto-protein levels in cases of primary liver cancer.


A rapid latex-agglutination test for invasive amoebiasis.


Clinical trials in radiotherapy and the merits of high energy protons.


High energy electron beam treatment planning for betatron 10-24 MeV.


A comparison of two methods for the isolation of Rubella virus.


Zinc environmental differences in carbonic anhydrase isozymes.


Specific microbiological techniques used in the infection control laboratory.


The toxicity of cochliodinol, an antibiotic metabolite of Chaetomium spp.


Diverticulosis of the small intestine and megaloblastic anemia.


An unidentified antigen present in abnormal amount in schizophrenic serum.


Purification and properties of biologically active factors in lipid extracts of Listeria monocytogenes.


Circulatory changes during stressful stimuli in rhesus monkeys.


Failure of calcitonin to prevent disuse osteopenia: An experimental study in rabbits.


The effect of threshold macular lesions and subthreshold macular exposures on visual acuity in the Rhesus monkey.


Corticosteroid clearance by the gastrointestinal tract in dogs and monkeys.


Nature of post vaccinal Brucella agglutinins.


Uterine bleeding in rhesus monkeys after intrauterine contraceptive device insertion: effect of adrenosem salicylate and p-aminomethylbenzoic acid.


Uterotrophic action of an intrauterine contraceptive device in Rhesus monkeys.


Problems in the bio-assay of orally administered cephaloglycin in biological fluids and method for the detection of its metabolite, desacetylcephaloglycin.


Composition of the cellular envelopes of Anabaena cylindrica. Comparative chemical analyses were made of the walls of vegetative cells, heterocysts, and spores, and of the mucilage of Anabaena cylindrica. The wall of the vegetative cell is composed predominantly of amino compounds, with a mannose-rich carbohydrate component comprising only 18% of the dry weight. In contrast, 62% of the heterocyst wall and 41% of the spore wall is carbohydrate. The carbohydrate moieties of the heterocyst wall and spore wall are similar in that the ratio of glucose, mannose, galactose, and xylose is approximately 75:20:3:4 in both walls. It appears that, during the differentiation of a vegetative cell into either a spore or a heterocyst, a glucose-rich wall polysaccharide is produced that is different from the polysaccharide component of the wall of the vegetative cell and of the sheath. In the case of the heterocyst, the wall was estimated to account for approximately 52% of the dry weight of the whole cell.


Activation energy for glucose-induced germination of Bacillus megaterium spores. The maximum germination rate of Bacillus megaterium QM B1551 spores in glucose increased, and the lag before its attainment decreased, with increasing germination temperature. The activation energy for germination (mu = approximately 20 kcal/mole), based on rate or on lag, was consistent with an enzymatic mechanism.


Early changes in the ultrastructure of Streptococcus faecalis after amino acid starvation. Thin sections of Streptococcus faecalis (ATCC 9790) starved of one essential amino acid (threonine or valine) initially show rapid increases in (i) cell wall thickness, (ii) the apparent size of the central nucleoid region, and (iii) mesosomal membranes. The most rapid increases in all three variables occurred during the first 1 to 2 hr of starvation. After this initial period, the rates progressively decreased over the 20-hr observation period. During threonine starvation, the mesosomal membrane that accumulated in the first hour was subsequently degraded and reached a level similar to that found in exponential-phase cells after 20 hr. With valine starvation, mesosomal membrane continued to slowly accumulate over the entire 20-hr observation period. The mesosomes of the starved cells retained the same "stalked-bag" morphology of those in exponential-phase cells. These cytological observations agree with previously published biochemical data on membrane lipid and wall content after starvation.


Effect of potassium chloride on the uptake and storage of phosphate by Saccharomyces mellis. The inorganic and polyphosphate pools of Saccharomyces mellis, grown in a medium containing excess phosphate, remain associated with the cells when the cells are suspended in a saline medium. If the cells are incubated in a medium containing 2 m KCl, the cells are altered in some manner which permits most of the orthophosphate and approximately one-third of the polyphosphate to be subsequently eluted by osmotic shock. At lower salt concentrations, beta-mercaptoethanol enhances this salt effect but is inactive by itself in this respect. At equivalent ionic strengths, the sodium salt of ethylenediaminetetraacetic acid behaves exactly like KCl or any other monovalent ionic compound in altering the cell to susceptibility to osmotic shock. No special effect of this anion at either high or low concentration could be detected. Resting cells are refractory to being altered in this manner by salts if an energy source, such as glucose, is included in the reaction mixture. Cells which are depleted of phosphate reserves will immediately incorporate phosphate when suspended in a medium containing inorganic phosphate and an energy source. These cells exhibit the phenomenon of "überkompensation." In resting cells, the inclusion of KCl in the reaction mixture prevents the conversion of orthophosphate into polyphosphate and, also, gradually decreases the ability of the organism even to assimilate orthophosphate. This effect is reversible, however, since the cells will incorporate phosphate in a normal manner if the cells are transferred to a non-salinized medium, or if a nitrogen source is included in the salinized reaction mixture so that the cells are now in a medium adequate for growth.


Lipid composition of Chlamydia psittaci grown in monkey kidney cells in defined medium. The lipid compositions of (i) monkey kidney (MK-2) cells cultivated in Eagle's minimal essential medium (MEM) with 5% calf serum, (ii) MK-2 cells cultivated in Waymouth medium supplemented with 20 mug of sodium oleate and 2 mg of bovine albumin per ml, (iii) Chlamydia psittaci strain 6BC grown in the latter host system, and (iv) calf serum were compared. Strain 6BC contains 31% phosphatidyl ethanolamine (PE) and 15% phosphatidyl glycerol (PG), whereas the host cell contains almost the same amount of PE (27%) and no PG. A high concentration of total lipid was observed in strain 6BC (29 to 34%), whereas MK-2 cells contain only 9 to 15% and calf serum contains 4.5% total lipid. The fatty acids of the total lipid from strain 6BC contain branched-chain acids. These fatty acids were found mostly in PE (33.0%) and PG (37.0%). No branched-chain fatty acid was found in the MK-2 cells. There was an increase in triglyceride content when MK-2 cells cultivated in MEM (19.2%) were compared with cells cultivated in Waymouth medium (28.0%). A high concentration (62.0%) of octadecenoic acid (C18:1) was found in the triglyceride of MK-2 cells cultivated in Waymouth medium. The level of polyunsaturated fatty acids observed in MK-2 cells cultivated in Waymouth medium (10.8%) and in the chlamydiae grown in these cells (13.3%) was low compared with the level in MK-2 cells (28.8%) cultivated in MEM with 5% calf serum and the level in calf serum itself (50.8%). A higher ratio of sterol ester to free sterol was found in calf serum than in MK-2 cells or in chlamydiae. Host contribution to lipid composition of strain 6BC is discussed.


Radiation sensitivity of Haemophilus influenzae: a composite response. The survival of ultraviolet (UV)-irradiated cultures of Haemophilus influenzae Rd is determined by at least two responses: (i) excision-repair ability and (ii) UV-induced cell lysis. An UV-resistant mutant, BC200, has the same capabilities as the wild type, Rd, for excising dimers but does not exhibit lysis. Lytic response is dose-dependent. Relative to the wild type, a lower dose of UV causes lysis of a UV-sensitive mutant, BC100, which is incapable of excising thymine dimers. A lytic protein is present in cultures undergoing lysis. Synthesis of this protein is initiated 45 to 60 min after irradiation. Lysis appears to be due to derepression of a defective prophage which codes for an endolysin-like lytic enzyme.


Derepression of tyrosinase synthesis in Neurospora by cycloheximide, actinomycin D, and puromycin.


The inactivation of Bacillus subtilis alpha-amylase by N-acetylimidazole and tetranitromethane.


Human carbonic anhydrases. 3. Immunochemical studies.


On the nonautoimmune aspects of endocardial fibroelastosis: continued inquiry into the significance of delayed sensitivity to mumps virus.


A nasojejunal tube for infant feeding.

A nasojejunal tube for infant feeding.


[GammaA in exercise proteinuria].

[GammaA in exercise proteinuria].


Change in plasma phosphate concentration on infusion of calcium gluconate or Na2EDTA.


Chicken immunoglobulins: biological half-lives and normal adult serum concentrations of IgM and IgY.


A social system model applied to a rehabilitation program for institutionalized patients.


Microbiological determination of the amino acids of xanthine oxidase.


Anamnestic antibody response in rabbits to topically applied 2,4-dinitrofluorobenzene.


The effect of progesterone and progesterone-estrogen on the glycogen deposition in the vagina of the squirrel monkey.


Innervational plasticity of the oculomotor system.


Efficacy of norbolethone in stimulating linear growth in stunted children.


A comparison of the antihypertensive effects of furosemide and chlorothiazide.


The effect of oral tetracycline HCl and doxycycline on the intestinal flora.


Preliminary studies of 6-(D-alpha-sulfoaminophenylacetamido) penicillanic acid (BL-P1462) in the treatment of Pseudomonas infections.


Gentamicin in childhood infections.

Gentamicin in childhood infections.


Clinical trial of thiothixene (Navane) in elderly chronic schizophrenics.


Clinical trial of clomacran phosphate (SKF-14336) in chronic psychotic male patients.


Preclinical evaluation of AHR-1118, a potential antidepressant drug.


Piperacetazine (Quide): a controlled evaluation of the elixir in chronic schizophrenic patients.


Uricosurics and xanthine oxidase inhibitors: a single-dose assay.


Serum insulin response in hemorrhagic shock in baboons.


Preparation and performance of a non-central nervous system animal.


The acute inhalation toxicity of monomethylhydrazine vapor. AMRL-TR-68-175.


Electron microscopic observations on the kidneys of animals. AMRL-TR-68-175.


Electron microscopic and morphometric study of rat, monkey and dog lungs exposed to 68 per cent O2 and 32 per cent N2 at 258 torr for eight months. AMRL-TR-68-175.


Comparative oxygen toxicity in sub-human primates. AMRL-TR-68-175.


Pulmonary lesions in monkeys exposed to 100 per cent oxygen at 258 and 750 mm Hg pressure. AMRL-TR-68-175.


Experience with the SMA-12 simultaneous multiple analyzer in animal toxicology. AMRL-TR-68-175.


Psychopharmacology of carbon monoxide under ambient and altitude conditions. AMRL-TR-68-175.


The fine structure of neuroglial cells and pericytes in the primate red nucleus and substantia nigra.


Removal from the small intestine of tubercle bacilli by macrophages in the guinea pig.


Epitheliocystis, a new infectious disease of the bluegill (Lepomis macrochirus).


Auerbach's plexus of mammals and man: electron microscopic identification of three different types of neuronal processes in myenteric ganglia of the large intestine from rhesus monkeys, guinea-pigs and man.


Ocular studies with para-chlorophenylalanine in rats and monkeys.


The effect of intraocular pressure on the pupil size.


Identification of "atypical" meningococcal strains in small children carriers, by means of the L.C.N. selective medium.


Crystalline and lamellar structures in LLCMK2 cell cultures persistently infected with dengue viruses.


Characterization of the factor deficient in the Hunter syndrome by polyacrylamide gel electrophoresis.


Removal of phenolics from plant extracts by grinding with anion exchange resin.


Disorders of the myenteric plexus.

Disorders of the myenteric plexus.


A study on resected cases of pulmonary tuberculosis with special reference to chemotherapy and healing.


A case of avian mastocytoma.

A case of avian mastocytoma.


The anatomy of the wrist joint.

The anatomy of the wrist joint.


A simple diagnostic milk medium for Pseudomonas aeruginosa. An agar medium containing 10% defatted milk has been tested as a diagnostic medium for Pseudomonas aeruginosa and, in particular, for differentiating between P. aeruginosa and P. fluorescens. Only P. aeruginosa colonies gave clear zones due to hydrolysis of casein together with diffused green pigment. Results on milk agar correlated well with the pattern of results from a variety of conventional tests used to identify this organism. Pigment production of P. aeruginosa on milk agar was better than on special media commonly used to enhance this characteristic. Routine diagnosis of P. aeruginosa is recommended by streaking on a solid medium containing 10% defatted milk granules, 25% nutrient broth, and 2% agar, and examining for clear zones and pigment after 24 hours' incubation.


Myasthenia gravis syndrome associated with trimethadione.


Syndromes of hyperviscosity.

Syndromes of hyperviscosity.


Emergency surgical operations for diverticular diseases.


Purification and serological reactions of four polyhedral viruses from Cruciferae.


Comparative study of methods for obtaining antigens (globulins) from seeds.


Further experiments on the glass tube method to control degeneration of potatoes.


Histological study of the topographic separation of thymus-type and bursa-type lymphocytes and plasma cell series in chicken spleen.


Histological changes in the genital tract of the female calf after administration of diethylstilbestrol and hexestrol.


Comparative morphological study of the major arterial supply to the pelvic limb of the domestic animals.


On the distributive patterns of alkaline phosphatase activity and their functional significance amongst the spinal ganglion cells of squirrel.


Studies of the composition and structure of plasma lipoproteins. Isolation, composition, and immunochemical characterization of low density lipoprotein subfractions of human plasma.


Ribonucleic acid sulfurtransferase from Bacillus subtilis W168. Sulfuration with beta-mercaptopyruvate and properties of the enzyme system.


Utilization of 14C-labelled Bacillus subtilis and Escherichia coli by sheep.


Microdiverticula and submucosal epithelial elements in ulcerative and granulomatous diseases of the ileum and colon. A case of granulomatous ileocolitis which also showed features of ulcerative colitis, and in which lesions were believed to be due to inflammation of previously undescribed structures, microdiverticula, was reported previously. Subsequently, sections of the segments of bowel received from 17 cases of regional enteritis and 16 cases of ulcerative colitis were re-examined for the presence of similar microdiverticula and submucosal epithelial elements. The same structures were present in five cases of regional enteritis and in 14 of ulcerative colitis. A granulomatous type of inflammatory response was found in the bowel and lymph nodes in 14 of the 17 cases of regional enteritis, and in none of the cases labelled ulcerative colitis. In spite of this, difficulty was experienced in placing some cases in one or other diagnostic category. This re-emphasizes the possibility that the two conditions may not be etiologically discrete, and that both may be, in fact, related to the presence of microdiverticula. In addition, it may be that colitis cystica profunda is a result of microdiverticulosis in which the submucosal glandular structures attain unusually large proportions.


Synthesis of tyrocidine B1.

Synthesis of tyrocidine B1.


Effect of EDTA on the distribution pattern of acetate-14C in rats.


Wall development and tetrazolium chloride reduction in heterocysts of blue-green algae, Anabaena ambigua.


Transport of whole protein molecules from blood to saliva of a plant bug.


Emphysema with hereditary alpha-1 antitrypsin deficiency masquerading as asthma.


A mutant of Bacillus subtilis appearing to have a temperature sensitive defect in the ribosomes.


Immunochemical studies on fluoresceinthiocarbamyl and reduced S-carboxymethylated cobrotoxin.


Treatment of typhoid and paratyphoid fever with nalidixic acid.


A revolution in medical care.

A revolution in medical care.


Circulatory L-asparaginase activity in primates.


Osteomyelitis: a review of clinical features, therapeutic considerations and unusual aspects. 3. Osteomyelitis associated with vascular insufficiency.


Modern steroid problems.

Modern steroid problems.


Macromolecular chemistry and medicine.

Macromolecular chemistry and medicine.


Some aspects of the active transport of amino acids.


Deficiency of dihydroxy acid dehydratase in the mitochondria of the iv-I mutants of Neurospora crassa.


A radioimmunoassay for quantifying carbonic anhydrase isozymes in crude lysates.


Heterogeneity in the silent gene phenotype of psudocholinesterase of human serum.


A possible relationship of neuroblastoma to Von Recklinghausen's disease.


Regression of coronary atheromatosis in rhesus monkeys.


Patterns of circulating luteinizing hormone and their relation to plasma progesterone levels during the menstrual cycle of the Rhesus monkey.


Interhemisphere imitation in split-brain monkeys.


Localization of function within the dorsolateral prefrontal cortex of the rhesus monkey.


Adaptation to visual field displacement by monkeys with optic chiasm sectioned.


Bloodtyping and twin zygosity.

Bloodtyping and twin zygosity.


Behavioral temperature regulation in the squirrel monkey: changes induced by shifts in hypothalamic temperature.


Effects of orbital frontal lesions on aversive and aggressive behaviors in rhesus monkeys.


Sex-linked coloboma in the chicken.

Sex-linked coloboma in the chicken.


The Federal Drug Abuse Program.

The Federal Drug Abuse Program.


Analysis of a family planning program in Guatemala.


Screening for galactosemia in New York State.


Sporothrix (Sporotrichum) schenckii in a nursery barn containing sphagnum.


Prevalence and causes of blindness in urban and rural areas of Egypt.


An apparent case of pesticide poisoning.


Influenza surveillance in Pennsylvania.

Influenza surveillance in Pennsylvania.


Readmission rates at Lexington Hospital for 43,215 narcotic drug addicts.


Demographic redefinition of Hispanos.

Demographic redefinition of Hispanos.


Use of paraprofessionals to motivate women to return for post partum checkup.


Detection of monoclonal IgM subunits in macroglobulinemia Waldenström.


Rabies in insectivorous bats in the United States, 1953-65.


Reproduction of temperature-sensitive strains of TMV under restrictive conditions in the presence of temperature-resistant helper strain.


Capillary permeability to human skeletal muscle measured by local injection of 51Cr-EDTA and 133Xe.


Enhancement of infectivity of tobacco mosaic virus particles partially uncoated by alkali.


Delayed lysis in Salmonella phage P22: the appearance of free fatty acids coincident with induced cell lysis.


Effects of altered external calcium concentrations on fluxes of calcium 45 in rat myometrium.


Hemagglutination by a nucleopolyhedrosis virus of the cotton bollworm, Heliothis zea.


Etiology and treatment of chronic socket discharge in patients wearing prosthesis.


Reversal of progesterone-enhanced insulin production by human chorionic somatommammotropin.


Regulation of gonadotrophin release by the corpus luteum in the baboon.


Pathogenesis of oncogenic simian adenoviruses. 8. The histopathology and ultrastructure of simian adenovirus 7-induced intracranial neoplasms.


Evaluation of the freeze-thaw alteration of rabbit male urogenital tissue antigens by double diffusion gel precipitation.


An electron microscopic study of human polymorphonuclear leukocyte injury induced by rabbit antiserum.


Effects of nitroglycerin on flow after partial constriction of the coronary artery.


Preliminary observations on the effect of synthetic thyrotropin releasing factor on plasma thyrotropin levels in man.


Important environmental factors in kidney preservation.


Comparative study on the anti-epileptic action of some common phyto-products.


The use of a pulsatile bloodless perfusion system in organ preservation.


The influence of hyperbaric oxygen toxicity on renal preservation.


The role of peroxidase vs. the role of tyrosinase in enzymatic conversion of tyrosine to melanin in melanocytes, mast cells and eosinophils.


Diagnosis of syndromes associated with hyperglobulinemia.


Diamine oxidase as an index of the fetoplacental unit. Clinical applications.


Effect of extraneous particulate matter on airborne bacteria.


Symptomatic gonorrhea during pregnancy.

Symptomatic gonorrhea during pregnancy.


A grid and isometric rulers for accurate needle placement during intrauterine transfusion.


Electron microprobe analysis of Ca, P, and Mg in mandibular bone.


Change in the trabecular pattern of the mandibular ramus in a Macaca rhesus monkey experimentally rendered edentulous.


The synaptic architecture of the hypothalamo-hypophyseal neuron systems.


Studies on the biology of the periodontium of marmosets: 8. The effect of folic acid deficiency on the marmoset oral mucosa.


Pheromones and behavior in mice.

Pheromones and behavior in mice.


Diabetes insipidus: its complex physiological basis and the relevance of cytochemical methods to the study of its pathogenesis.


The distribution of antibacterial agents between plasma and lymph in the dog. 1. Plasma, peripheral and thoracic lymph concentrations of penicillin V, phenethicillin, carbenicillin, ampicillin, cloxacillin, penicillin G, chloramphenicol and sulphadiazine were determined at various time intervals up to 6 h following intramuscular administration of 50 mg/kg to dogs.2. Peak plasma concentrations of the penicillins occurred within half an hour after administration with the peak lymphatic concentrations occurring 1.5 to 3 h afterwards. For the remaining period of the test the concentration in the lymph exceeded the corresponding concentration in the plasma. Sulphadiazine gave concentrations in thoracic lymph equal to the plasma concentration, but the peripheral lymph concentrations were lower while the concentrations of chloramphenicol in both peripheral and thoracic lymph were always lower than the plasma concentrations.3. After the peak concentrations were reached, the concentration curves for penicillins in lymph followed the same pattern as found in plasma, the penicillin concentrations declining exponentially. Sulphadiazine produced more persistent levels both in lymph and in plasma while the concentrations of chloramphenicol were still rising 6 h after administration.4. The free concentrations of penicillin in lymph were equal to the free concentrations in plasma, whereas the concentrations of free sulphadiazine and chloramphenicol in lymph were less than those in the plasma.


Productive and abortive growth of an oncogenic simian adenovirus SV20 in cultured cells.


Histopathology of tumors and cultivation of tumor cells derived from simian adenovirus SV20-infected hamsters.


Histologic evaluation of ophthalmoscopically subvisible retinal laser exposures.


Immunochemical quantitation of human Cl esterase (Cls): concentration in normal serum and purification yields.


Metabolism of gamma G and gamma M immunoglobulins in normal and hypogammaglobulinemic chickens.


Characteristics of the in vitro response of guinea pig blood lymphocytes to PHA and antigen.


Gamma G erythrocyte autoantibodies: comparison of in vivo complement coating and in vitro "Rh" specificity.


The infant Temptrol oxygenator.

The infant Temptrol oxygenator.


Secretory IgA in human serum.

Secretory IgA in human serum.


Development and changes of annulate lamellae complexes in rubella virus-infected RK-13 cells.


Waldenström's macroglobulinaemia with high-titre rheumatoid factor treated with D-Penicillamine.


Failure of transplanted cells from young chicks to synthesize their allotype.


Pathology of renal allografts in patients receiving long-term antilymphocyte globulin therapy.


Allotype synthesis by adult donors' leukocytes and spleen cells injected into newly hatched chicks.


Experimental studies on leukemia in guinea pig.


Gastric mucosa in iron deficiency anaemia. Results of follow-up examinations.


The osmotic fragility and density distribution of erythrocytes in the newborn.


The osmotic fragility of red cells in newborns and infants.


In vitro cultivation of Plasmodium falciparum.


Lassa fever, a new virus disease of man from West Africa. II. Report of a laboratory-acquired infection treated with plasma from a person recently recovered from the disease.


Lassa fever, a new virus disease of man from West Africa. IV. Electron microscopy of Vero cell cultures infected with Lassa virus.


Lassa fever, a new virus disease of man from West Africa. 3. Isolation and characterization of the virus.


An outbreak of Lassa fever on the Jos plateau, Nigeria, in January-February 1970. A preliminary report.


Matucare virus, a new agent recovered from Ornithodoros (Alectorobius) boliviensis.


Recovery of dengue-2 and dengue-3 viruses from man in Jamaica.


Exogenous interferon in animals and its clinical implications.


Polymorphism of mycobacteria and shifts of host immunity.


Rifampicin in the retreatment and original treatment of advanced pulmonary tuberculosis.


Evaluation of nitroglycerin in angina in patients on isosorbide dinitrate.


Fine structure features of monkey (Macaca mulatta) reduced enamel epithelium.


Tooth drift and changes in the temporomandibular joint following tooth extraction in the monkey.


Sterilization of tuberculous lesions in man.


Pharmacology of a new analgesic, antipyretic, and anti-inflammatory agent, 4-allyloxy-3-chlorophenylacetic acid.


Toxicity studies of 4-allyloxy-3-chlorophenylacetic acid, a new analgesic, antipyretic, and anti-inflammatory agent.


Metabolic patterns of 4-allyloxy-3-chlorophenylacetic acid in rat, rabbit, dog, monkey, and man.


Bacterial flora of the bovine eye with special reference to the Moraxella and Neisseria.


Bovine ephemeral fever: the reaction of cattle to different strains of ephemeral fever virus and the antigenic comparison of two strains of virus.


Conformational changes in chymotrypsin detected with antibodies. I. Antibody response to diisopropylphosphoryl-chymotrypsin.


The first phase of pseudomonadal pneumonia influenced by the administration of ampicillin + EDTA in vivo.


Serum corticosterone levels in rats at various time intervals after alloxanization.


Clinical pharmacology scope, organization, training. Report of a WHO study group.


Experiences in assessing fetal acid-base state.


Antepartum and postpartum use of passive Rh antibodies.


Assessment of fetoplacental function by hormone assay.


The AABB reference laboratories.

The AABB reference laboratories.


Taxonomic studies on some gram negative polarly flagellated "hydrogen bacteria" and related species.


Major paraffin constituents of microbial cells with particular references to Chromatium sp.


Control mechanisms of cholesterol biosynthesis.


Intensity changes at the ear as a function of the azimuth of a tone source: a comparative study.


Maintenance of anabolism by total parenteral nutrition in patients.


Conversion of bacterial aldolase from vegetative to spore form by a sporulation-specific protease. A protease is elaborated by cells of Bacillus cereus during sporulation. This sporulation-specific enzyme, in a limited proteolysis, converts vegetative cell aldolase to spore aldolase.


A variable region subclass of heavy chains common to immunoglobulins G, A, and M and characterized by an unblocked amino-terminal residue. Heavy chains of IgG, IgA, and IgM classes of human immunoglobulins were compared by N-terminal residue determination and partial amino acid sequence analysis. A third subclass of the variable region of heavy chains was observed; an unblocked glutamic acid as the N-terminal residue is characteristic of this subclass. Our results indicated that the heavy chain variable region subclasses are not class specific, and that a given heavy chain variable region may be found in association with constant regions for mu-chains, alpha-chains, or gamma-chains of various subclasses.


Translational complementarity of RNA transcripts of native and denatured B. subtilis DNA. Previous evidence has suggested that the L and H fractions into which denatured DNA of B. subtilis can be separated by chromatography are complementary to each other with regard to base compositon, nucleotide sequence, and template properties, and that they may be regarded as families of fragments of the respective DNA strands. The present study tests the proposition that these fractions should also exhibit features of translational complementarity with respect to the DNA-dependent or the RNA-dependent incorporation of amino acids into ribosomes. This has been shown to be the case with the use of two pairs of amino acids: (1) lysine and phenylalanine; (2) proline and glycine.


Directional character of proton transfer in enzyme catalysis. Hydrogen bonding can facilitate proton transfer in certain directions and retard proton transfer in certain other directions. By assuming that directed proton transfer along strategically oriented hydrogen bonds in the enzyme-substrate complex plays an important role in determining the efficiency and specificity of the enzyme, we present a unified interpretation for the reported observations on carbonic anhydrase and alpha-chymotrypsin.


Immunoglobulin components in crystalline ceruloplasmin and anticomplementary activity.