Effects of temperature on activation, germination, and outgrowth of Bacillus megaterium spores. The effects of temperature on the activation, glucose-induced germination, and outgrowth of Bacillus megaterium QM B1551 spores were investigated. There was no evidence for discontinuities in the response of spores to temperature in these processes reflecting reported thermal anomalies in the physical structure of water. Increasing the temperature of heat activation (aqueous suspensions, 5 min) increased the germinability of spores. Activation, as measured by extent of germination, was optimal after heating at 62 to 78 C, and the rate of spore germination was maximal after heat activation at 64 to 68 C. Increasing the temperature of activation above 68 C depressed the germination rate and increased the time lag before this rate was reached. Germination occurred over a wide range of temperatures, but was optimal between 28 and 38 C. The highest rate of germination was at 38 C; at lower incubation temperatures, the maximum attained rate was lower and the lag in attaining this rate was extended. Outgrowth (postgerminative development through the first cell division) of the germinated spores in Brain Heart Infusion (BHI) occurred in at least two phases-a temperature-dependent lag phase followed by a relatively temperature-independent phase of maximum outgrowth rate, during which increase in optical density was a linear function of time. Outgrowth time (time required for doubling of the initial optical density), essentially dependent on the time for completion of the lag phase, was shortest at temperatures between 34 and 40 C. The temperature-dependent lag phase was completed in a rich medium (e.g., BHI) but not in the glucose germination medium, suggesting that the endogenous reserves of the germinated spore were inadequate to support the metabolic synthetic events occurring during this period.


Ossification of the Achilles tendon. Report of seven cases.


Ossification of a lumbar interspinous ligament with compression of the cauda equina. A case report.


Gargoylism: hydrolysis of beta-galactosides and tissure accumulation of galactose- and mannose-containing compounds. The sugars present in hydrolyzed extracts of human liver and brain were analyzed by gasliquid chromatography after conversion to their alditol acetates. The samples analyzed were obtained from control subjects, patients with gargoylism, and patients with a few other kinds of storage disorders. Accumulation of galactose was demonstrated in the liver and the brain of two patients with gargoylism, and in the liver samples, high levels of mannose were found too. We also studied the hydrolysis of a number of galactosides by homogenates from different tissues in the control subjects and in the patients. Separation methods and kinetic studies demonstrated the presence in normal human tissues of two different beta-galactosidases, which we call enzyme A and enzyme B, respectively. Enzyme A hydrolyzed all the beta-galactosides tested. Enzyme B hydrolyzed the synthetic substrates tested (4-methylumbelliferyl-, p-nitrophenyl-, o-nitrophenyl-, and phenyl-beta-galactoside) but not the natural substrates tested (ceramide-beta-galactoside, ceramide lactoside, transferrin glycopeptide, and keratan sulfate). Enzyme B also exerted beta-glucosidase activity. In various tissues from patients with gargoylism, deficiency of beta-galactosidase A could be demonstrated. It is suggested that the high level of galactose found in the hydrolyzed extracts of tissues from gargoylism patients is due to storage of galactose-rich glycosaminoglycans and glycopeptides, and that this storage is a result of the deficiency of beta-galactosidase A. The high level of mannose in the liver from gargoylism patients seems to indicate storage of glycopeptide, adding a new group of substances to those known to be stored in gargoylism.


Effects of controlled interruption of the enterohepatic circulation of bile salts by biliary diversion and by ileal resection on bile salt secretion, synthesis, and pool size in the rhesus monkey. The effects of controlled interruption of the enterohepatic circulation (EHC) of bile salts by biliary diversion on bile volume, bile salt secretion and synthesis rates, bile salt pool size, and the relationship to fecal fat excretion were studied in 16 rhesus monkeys. Bile from a chronic bile fistula was returned to the intestine through an electronic stream-splitter which, by diverting different percentages of bile to a collecting system, provided graded and controlled interruption of the EHC. The increase in hepatic bile salt synthesis in response to interruption of the EHC was limited and reached a maximum rate at 20% interruption of the EHC. Up to this level of biliary diversion, the increased hepatic synthesis compensated for bile salt loss so that bile salt secretion and pool size were maintained at normal levels. With diversion of 33% or more, there was no further increase in hepatic bile salt synthesis to compensate for external loss, and as a result there was diminished bile salt secretion, a reduction in bile salt pool size, and steatorrhea was observed. The effects of interruption of the EHC by the streamsplitter were compared with those produced by resection of the distal one-third or two-thirds of small bowel. While ileal resection appreciably reduced bile salt secretion, the EHC was by no means abolished. Bile salt reabsorption from the residual intestine was greater after one-third than after two-thirds small bowel resection. These observations suggest that jejunal reabsorption of bile salts occurs and may well contribute to the normal EHC.


Characterization of human anti-glomerular basement membrane antibodies eluted from glomerulonephritic kidneys. Eluates from glomerulonephritic kidneys of nine patients with anti-glomerular basement membrane (anti-GBM)-mediated nephritis were studied to define their antigenic specificity and content of kidney-fixing antibodies. Five of these patients had Goodpasture's syndrome with pulmonary and renal involvement clinically; four patients did not. All had in vivo fixation of IgG in the characteristic linear pattern by direct immunofluorescence, and eluted IgG fixed to normal human kidney sections. Eluates from kidneys of patients with Goodpasture's syndrome fixed more frequently to homologous nonglomerular renal and extrarenal antigenic sites and to heterologous GBM than did non-Goodpasture eluates over a hundredfold range of antibody concentrations; both could be blocked by prior absorption with soluble GBM antigens. By radial immunodiffusion and precipitation tests the content of IgG in the eluates was measured to range from 2 to 20% of the total protein eluted. By paired label isotopic fixation studies with some of the eluates the per cent of IgG that was kidney fixing ranged from 0.6 to 23.4%. Although the in vivo fixation studies with radiolabeled eluates failed to indicate significant fixation to monkey lung, the observations define quantitative as well as qualitative differences between anti-GBM antibody populations mediating the Good-pasture syndrome compared to those causing glomerulonephritis without lung involvement.


Ionized calcium in normal serum, ultrafiltrates, and whole blood determined by ion-exchange electrodes. Ion-exchange calcium electrodes represent the first practical method for the direct measurement of ionized calcium [Ca(++)] in biologic fluids. Using both "static" and "flow-through" electrodes, serum [Ca(++)] was within a rather narrow range: 0.94-1.33 mmoles/liter (mean, 1.14 mmoles/liter). Within a given individual, [Ca(++)] varied only about 6% over a several month period. Consistent pH effects on [Ca(++)] were observed in serum and whole blood, [Ca(++)] varying inversely with pH. Less consistent pH effects were also noted in ultrafiltrates, believed to largely represent precipitation of certain calcium complexes from a supersaturated solution. Heparinized whole blood [Ca(++)] was significantly less than in corresponding serum at normal blood pH, related to the formation of a calcium-heparin complex. [Ca(++)] in ultrafiltrates represented a variable fraction (66.7-90.2%) of total diffusible calcium. There was no apparent correlation between serum ionized and total calcium concentrations. Thus, neither serum total calcium nor total ultrafiltrable calcium provided a reliable index of serum [Ca(++)]. Change in serum total calcium was almost totally accounted for by corresponding change in protein-bound calcium [CaProt]. About 81% of [CaProt] was estimated to be bound to albumin and about 19% to globulins. From observed pH, serum protein, and [CaProt] data, a nomogram was developed for estimating [CaProt] without ultrafiltration. Data presented elsewhere indicate that calcium binding by serum proteins obeys the mass-law equation for a monoligand association. This was indicated in the present studies by a close correspondence of observed serum [Ca(++)] values with those predicted by the McLean-Hastings nomogram. While these electrodes allow study of numerous problems not possible previously, they have not been perfected to the same degree of reliability obtainable with current pH electrodes. The commercial (Orion flow-through) electrode is: (a) expensive. (b) requires periodic replacement of membranes, and (c) has not yet been thermostated. As with blood pH measurements. (d) electrode response is logarithmic, i.e. small potential errors generate rather large [Ca(++)] errors. (e) loss of CO(2) should be prevented, and (f) errors due to other cations must be considered under certain conditions. Despite these limitations, we believe the electrode represents a major advance in calcium metabolism.


Lytic properties of bacterial cultures of Pseudonomas aeruginosa. I. Spontaneous lytic properties.


Smooth muscle in the ovary of the rat and monkey.


Functional results in the reconstruction of bony meatus after radical mastoid operation.


Dose-response relationship between inoculum dose of anthrax spores and survival time of mice.


Pathogenicity, immunogenicity and distribution of transmissible gastroenteritis virus in pigs.


Comparative studies in lung mechanics based on a survey of literature data.


Neuroglia: gliosis and focal epilepsy.

Neuroglia: gliosis and focal epilepsy. Normal neuroglial cells buffer the extracellular space around neurons and presynaptic terminals against increases in potassium ions. Epileptic foci resulting from brain injury are characterized by areas of intense fibrillary gliosis bordering neuronal tissue. The known pathological changes that occur in gliosis may impair glial control of extracellular potassium ions and lead to excessively excitable neuronal border regions.


Penicillin as epileptogenic agent: its effect on an isolated neuron. Penicillin induces partial depolarization and increased excitability of the neuronal membrane of crayfish stretch receptor. Such effects suggest that the epileptic focus created by the topical application of penicillin to the mammalian cerebral cortex may result from the lowering of the threshold for impluse initiation by excitatory synaptic action within the neuron population.


Distribution of ABO and Rh blood groups among Cree Indians at Fort George, Quebec.


Nortriptyline (Aventyl) overdosage.

Nortriptyline (Aventyl) overdosage.


Dual control of megateriopeptidase synthesis.


Hemodynamics in Chagas' disease. A preliminary report.


Synaptic patterns in the dorsal lateral geniculate nucleus of the monkey.


Polyacrylamide-gel electrophoresis of apple-seed enzymes.


Dissociation and reconstitution of 12-S toxin of Clostridium botulinum type E.


Resolution of polypeptide chains of sulfitolyzed fibrinogen by polyacrylamide gel electrophoresis.


Synthesis of diribonucleoside monophosphates by use of a nonspecific ribonuclease from Bacillus subtilis.


Enrichment of Bacillus subtilis DNA in the sequences for ribosomal RNA.


High-molecular-weight artifacts in RNA extracted from Blastocladiella at elevated temperatures.


Changes in the sedimentation properties of HeLa cell DNA and nucleoprotein during replication.


Nucleotide sequence of valine tRNA 1 from Escherichia coli B.


In vivo bactericidal activity of rifampicin in combination with other antimycobacterial agents.


Cardiac auto-antibodies. II. Characterization, partial purification and attempted identification of the rabbit heart auto-antigens.


Lysis of chicken erythrocytes sensitized with PPD by lymphoid cells from guinea pigs immunized with tubercle bacilli.


Cytochromes of Bacillus subtilis. II. Purification and spectral properties of cytochromes c-550 and c-554.


Physical and chemical properties of amyloid fibers. II. Isolation of a unique protein constituting the major component from human splenic amyloid fibril concentrates.


Metabolism of sphingolipids by normal and atherosclerotic aorta of squirrel monkeys. We studied the synthesis and hydrolysis of sphingomyelin by homogenates of aortic intima plus inner media from normal squirrel monkeys and from monkeys with nutritionally-induced atherosclerosis (6-10 mo on a semi-purified diet containing butter and cholesterol). The concentrations of sphingomyelin in the aortas and plasmas of the atherosclerotic monkeys were higher than those for the normal monkeys. Palmitoyl-1-(14)C coenzyme A was actively utilized for the synthesis of ceramide (N-palmitoyl sphingosine). The addition of sphingosylphosphorylcholine increased the utilization of palmitoyl CoA in sphingomyelin synthesis, and the addition of psychosine (sphingosyl galactoside) increased the incorporation of palmitate into cerebrosides. Rates of sphingomyelin and ceramide synthesis were significantly higher in the atherosclerotic than in the control aortas. Hydrolysis of labeled sphingomyelin to ceramide was also increased in homogenates of the atherosclerotic aortas. Labeled sphingomyelin was taken up from plasma by everted carotid arteries, and this process was also enhanced by atherosclerosis. Increased rates of synthesis and of uptake from plasma of sphingomyelin may account for the increased concentrations of sphingomyelin in the atherosclerotic arteries, even though the ability to degrade sphingomyelin is also enhanced in the atherosclerotic aorta.


Transformation and transduction in Bacillus subtilis: evidence for separate modes of recombinant formation.


Effects of ergot alkaloids (hydergine) on cerebral hemodynamics and oxygen consumption in monkeys.


Influence of the composition of Freund's adjuvant, strain and sex of animals on the course of adjuvant--induced polyarthritis in rats.


Survival of algae under adverse conditions.


Developmental changes accompanying the breaking of the dormant state in bacteria.


Folate metabolism and virulence of mycobacteria.


Isoniazid uptake and growth inhibition of Mycobacterium tuberculosis in relation to time and concentration of pulsed drug exposures.


The primary isolation of tubercle bacillus and Staphylococcus pyogenes from bone lesions.


Studies of a foeto-specific protein in rabbits. Absence of its relation to the human alpha-foetoprotein.


Excretion in urine of metabolites of glyceryl trinitrate (trinitrin) in rabbits.


Bacteriological, pharmacological, and clinical studies of carbenicillin.


Effect of oligomygin on proton translocation in submitochondrial particles.


The demineralization and embedment of bone specimens.


The cellular localisation of glutathione peroxidase and its release from mitochondria during swelling.


Investigation of the mating system of Pseudomonas aeruginosa strain 1. V. The effect of N-methyl-N'-nitro-N-nitrosoguanidine on a donor strain.


DNA synthesis in Lactobacillus acidophilus in the absence of RNA and protein synthesis: a study with rifampicin.


EEG in focal fits.

EEG in focal fits.


"...one giant leap..."?

"...one giant leap..."?


Prevention of meningococcal disease by group C polysaccharide vaccine.


The reaction mechanism of N-acetylglutamate-5-phosphotransferase from Chlamydomonas Reinhardti.


Observations on the in vitro bactericidal activity of sera from pre-natal and post-natal calves.


Effects of nitroglycerin upon the presystolic gallop.


Polycythemic donors--are they useful and safe?


Localization of rabbit intestinal sucrase with ferritin-antibody conjugates.


Effect of infection with RNA phage R23 on membrane permeability and K+ fluxes in Escherichia coli.


Free amino acid composition of some nitrogen fixing blue-green algae in heterocystous and non-heterocystous conditions.


Application of the electroprecipitin test to the detection of the virus of canine distemper.


Intestinal IgA in the pig.

Intestinal IgA in the pig.


A cell culture assay to evaluate the toxicity of arlacel A.


A descending vesicopressor pathway in the monkey.


Carbohydrate-containing group-specific antigens of Neisseria meningitidis.


Luteolytic effects of prostaglandin F2a in primates.


Circadian rhythms of plasma 17-hydroxycorticosteroids in the infant monkey.


19S antibodies as mediators of pulmonary edema produced in rats by nephrotoxic serum.


Steric, electronic and integrative factors in enzymic regulation.


The distribution and excretion of tritium labeled cerebroside (galactosyl ceramide) radioactivity by the monkey.


Aspects in production of antilymphocyte sera.


In vivo evaluation of antihuman lymphocyte sera.


Immunohistochemical localization of creatine phosphokinase in skeletal muscle. A specific antiserum against skeletal muscle creatine phosphokinase has been obtained by immunizing chickens with purified rabbit skeletal muscle creatine phosphokinase. The antiserum was organ- rather than species-specific and reacted equally well with creatine phosphokinase from rabbit, guinea pig, and human skeletal muscle. Immunological specificity was verified by double immunodiffusion in agar gel where the precipitin line exhibited creatine phosphokinase activity. In solution the antiserum inhibited the enzymatic activity of creatine phosphokinase. By means of the immunofluorescent technique, creatine phosphokinase was localized in the intermyofibrillar space of normal human skeletal muscle fibers in cryostat sections. A higher concentration of the enzyme was found in fibers which have primarily an anaerobic enzyme profile (type II fibers) as determined by conventional histochemical reactions.


Aging of alpha-crystallins during development of the lens. Fetal alpha-crystallin from the bovine lens can be resolved into three major subunit fractions by ion exchange chromatography. A fourth subunit gradually arises during the development of the lens. Incorporation studies indicate that this subunit is not formed by de novo synthesis but by the chemical conversion of one subunit into another. Possible mechanisms for this conversion are discussed.


Psychological approaches to opiate dependence and self-administration by laboratory animals.


Intravascular thrombosis and leukocyte destruction in vivo by heterologous antilymphocyte serum.


Quantitative comparison of the intensity of Schistosoma mansoni infections in man and experimental animals.


Experimental approaches tohomogenous antibody populations. iIndividual antigenic specificity, cross specificity and diversity of human antibodies.


Immunological studies on simian malaria parasites. IV. Heterologous superinfection of monkeys with chronic Plasmodium knowlesi infections.


Quantitative studies of idiotypic antibodies.


Experimental approaches to homogenous antibody populations. Mouse IgA myeloma proteins that bind polysaccharide antigens of enterobacterial origin.


Experimental studies on the antigenic action of bioplast preparations.


Experimental infection of rhesus monkeys with Högyes' fixed strain of rabies virus.


The isolation of tubercle bacilli from endocervical microcurettings of 100 consecutive infertile women.


Pheromones.

Pheromones.


Mechanisms of action of intra-uterine contraceptive devices in women and other mammals.


Gamma-glutamyl phosphate attached to glutamine-specific tRNA. A precursor of glutaminyl-tRNA in Bacillus subtilis.


Slow virus infections of the nervous system.


The subunit structure of alpha-crystallin. Isolation and characterization of the S-carboxymethylated acidic subunits from adult and embryonic origin.


Nondialyzable urinary hydroxyproline as an index of bone collagen formation.


Studies of experimental brucellosis. Brucella abortus infection in the male guinea pig and effect of sensitization on various organs.


Reversible plateau v.s. spike action potential of amphibian cardiac muscle fiber.


Pulmonary Function. A study in the baboon using xenon-133 and a scintillation camera.


Combined carbenicillin and gentamicin for prophylaxis of post-operative infection following major abdominal surgery.


Quantitative studies on bovine immunoglobulins. I. Adult plasma and colostrum levels.


Pericanalicular rod-shaped bodies.

Pericanalicular rod-shaped bodies.


Studies on the in vitro bactericidal activity of bovine sera against selected udder pathogens.


Planning intravenous alimentation of surgical patients.


Age and sex incidence of ankle fractures.


Coronal cleft vertebrae in growing individuals. A preliminary report.


A double-linear immunodiffusion method for the quantitation of proteins.


Effect of a single massive dose of vitamin A on serum and liver levels of the vitamin.


The density of tissues in and about the head.


Equine immunoglobulins: a comparison of molecular properties.


Some recent studies in the Nutrition Research Laboratories, Hyderabad.


Human platelet membrane protein.

Human platelet membrane protein.


Immunochemistry of staphylococcal nuclease. I. Physical, enzymatic, and immunological studies of chemically modified derivatives.


Biosynthesis of hydrocarbons in Anabaena variabilis. Incorporation of [methyl-14C]- and [methyl-2H3]methionine into 7- and 8-methylheptadecanes.


Phosphorylation of 1-alkenyl-2-acylglycerol and preparation of 2-acylphosphoglycerides.


Studies on alpha albumin in nervous tissue. I. Biochemical investigations.


Neural correlates of stimulus response and reinforcement.


Inhibition of the eighth component of complement (C8) by ethylenediaminetetra-acetate (EDTA).


Spontaneous thyroiditis in the obese strain of chickens. V. The effect of sublethal total body X-irradiation on the development of the disease.


Antibody formation in zwoegerziekte, a slow infection in sheep.


Release of Fab- and Fc-like fragments from human IgM by reductive cleavage.


Nonimmunologic precipitin lines between serum and enteric contents giving false-positive evidence of local antibody production.


Release from Escherichia coli of a galactosyltransferase complex active in lipopolysaccharide synthesis.


Tissue culture techniques in the study of cell photobiology and phototoxicity.


Lack of synergy of EDTA with antimicrobials in resistant enterobacteriaceae.


Complete mutagenesis in a bacterial population induced by thymine starvation on solid media.


The definition and measurement of health and disease.


Vertebrate ecology in public health.

Vertebrate ecology in public health.


Evaluation of motivation of patients in a streptococcal control program.


The insurance industry looks ahead at health care in the 1970's.


Survey of coronary care facilities in 50 Massachusetts hospitals.


The federal health agency as preceptor in community medicine.


Establishing family planning services in Kenya. The history and present status of family planning services in Kenya are reviewed.  12 references are cited.  Kenya was the first African country south of the Sahara to adopt family planning as a national policy.  Voluntary and government efforts and their interaction are detailed.  There is a growing rate of acceptance of modern contraception in this nation which is 93% rural and newly independent.  The following steps should be implemented to develop an effective family planning program resulting from a national policy decision:  (1) demographic data for analysis and prediction should be gathered by an analytical unit; (2) government  must firmly adopt a policy to be carried out by the civil service; (3) for orientation to policy and technological training, courses for teaching and reteaching should be instituted; (4) an organization and management system which is secure  financially with assured personnel should be started; (5) a feedback of data to the analytical unit for purposes of policy, training, and program modification should occur.


Quality clinical laboratory services for the American people.


Gonorrheal urethritis in males treated with a single oral dose of minocycline.


Use of the emergency room in a community hospital.


Epidemiology of beef tapeworm infection in the United States.


Using workers from "hard-core" areas to increase immunization levels.


Mission of the Health Services and Mental Health Administration.


Quantitative measurement of the increase in dose adjacent to voids during irradiation of a lucite phantom by high-energy electron beams.


Histological and histochemical examinations in experimental staphylococcal enterotoxin poisoning.


The reactions of cord blood with saline agglutinating anti-Rho(D) serum.


Radium and external irradiation versus radium and operation for early invasive carcinoma of the uterine cervix.


Endometriosis in rhesus monkeys.

Endometriosis in rhesus monkeys.


Bilirubin estimation in amniotic fluid. A comparative study of 60 cases.


The management of the teeth, bone, and soft tissues in patients receiving treatment for oral cancer.


Plasma diamine oxidase in pregnancy complicated by age and weight factors.


Radical radiation therapy of chordoma.

Radical radiation therapy of chordoma.


Complications in 831 patients with squamous cell carcinoma of the intact uterine cervix treated with 3,000 rads or more whole pelvis irradiation.


Epidemiological aspects of epilepsy in Nigeria; a study of 639 patients.


Amino acid transport and incorporation in red blood cells of normal and Plasmodium knowlesi-infected rhesus monkeys.


Antigenic analysis of developmental stages of Ascaris suum. I. Comparison of eggs, larvae and adults.


Studies with antibodies to gastrin. Radioimmunoassay in human serum and physiological studies.


Some effects of preservatives on the development of bacterial spores.


Cross-reactivity in primary respiratory allergy to castorbean (Ricinus communis).


Media selective for Listeria monocytogenes.


Kinetic analyses of calcium movements in cell cultures. 3. Effects of calcium and parathyroid hormone in kidney cells. Calcium compartments and fluxes were measured by kinetic analyses in kidney cell suspensions in a three-compartment closed system. The fast phase influx and compartment size increase linearly with the medium calcium and the half-time of exchange is only 1.3 min which suggests that the fast component is extracellular. The slow phase compartment rises linearly from 0.1 to 0.5 mmole calcium/kg cell water when the medium calcium is raised from 0.02 to 2.5 mM. The slow phase calcium influx exhibits the pattern of saturation kinetics with a V(max) of 0.065 micromicromole cm(-2) sec(-1) and a K(m) of 0.3 mM indicating that it is a carrier-mediated transport process. PTH has no effect on the fast phase of calcium influx, but increases both calcium influx and the calcium pool size of the slow component. The maximum effect is obtained at medium calcium concentration of 1.3 mM. Below 0.3 mM extracellular calcium, the effects of the hormone cannot be demonstrated. PTH increases the V(max) of calcium influx from 0.065 to 0.128 micromicromole cm(-2) sec(-1) while the K(m) rises from 0.3 to 1.15 mM. These findings suggest that PTH increases the translocation of the calcium-carrier complex across the membrane and not the carrier concentration or its binding affinity for calcium.


The life cycle of Nosema bombycis as revealed in tissue culture cells of Bombyx mori.


Calcium efflux from frog twich muscle fibers.


Host-pathogen relationships of two previously undescribed microsporidia from the Indian-meal moth, Plodia interpunctella (Hübner), (Lepidoptera: Phycitidae).


The occurrence of chitin in the spore wall of Glugea weissenbergi.


Intracellular stages in the life cycle of the microsporidian Nosema apis.


Two eugregarina, Gregarina sp. and Actinocephalus sp., associated with the scarab Phyllophaga anxia, as observed in histological sections.


A suggested role for the polyphenol-phenoloxidase system in invertebrate immunity.


Transmission of serum hepatitis.

Transmission of serum hepatitis.


You can't change the system without a solid push, and there isn't any push.


Fine-particle humidifiers. Source of Pseudomonas aeruginosa infections in a respiratory-disease unit.


White-cell beta-galactosidase activity.

White-cell beta-galactosidase activity.


Serological differentiation of human, orang-utan, gibbon, monkey and baboon bloods.


Fine structure of toxigenic and non-toxigenic Corynebacterium diphtheriae.


Studies on mycobacterial sensitizing fractions. II. Variations in protein content of culture filtrates of different human strains of M. tuberculosis.


Effect of glyceryl trinitrate (nitroglycerin) on the systemic and coronary circulation of the dog.


Repetitive firing produced by pacemaker stimuli falling after the T wave.


Cochlear potentials inside the cochlear duct at the level of the round window.


Accidental cyanide poisoning.

Accidental cyanide poisoning.


Effect of brilliant green on the motility of Salmonellae and other selected microorganisms. The effect of Brilliant Green on motility was studied with Salmonella anatum, S. derby, S. tennessee, Escherichia coli, Proteus vulgaris, and Pseudomonas aeruginosa. Semisolid tryptic soy-agars containing 0, 20, or 40 mg of Brilliant Green per liter were used as the motility media. Both concentrations of Brilliant Green inhibited the growth of the non-Salmonella species through the semisolid agar. For 24 hr, the Brilliant Green appeared to limit the growth of the salmonellae; however, by 48 hr the salmonellae were able to grow through the semisolid agars. The presence of Brilliant Green in the motility media aided in the detection of Salmonella when mixed cultures were used.


Occurrence of false-positive most probable number tests for fecal streptococci in marine waters. By the use of the most probable number technique with azide dextrose and ethyl violet azide broths for enterococci, the common occurrence of false-positive tests was noted when marine and estuarine waters were sampled. Organisms isolated included a marine bacterium, gram-positive and gram-negative nonmarine bacteria, and yeasts. All cultures were capable of growth in azide-dextrose, ethyl violet-azide, and KF broths. Representative isolates grew in media containing 0.08% NaN(3). The tentatively accepted most probable number method for fecal streptococci is thus of dubious value in assessment of sewage pollution levels in estuarine waters. All positive tubes must be examined microscopically for the presence of nonstreptococcal forms.


Immunofluorescent detection of enterotoxin B in food and a culture medium. Both Staphylococcus aureus strains 243 and S-6 cells producing enterotoxin B and free enterotoxin in food and culture medium were rapidly demonstrated by using the fluorescent-antibody technique. Comparison of cell fluorescence and enterotoxin B production determined by double gel diffusion showed that an estimation of enterotoxin production could be made by observing the degree of cell fluorescence. The fluorescent-antibody technique was used to determine whether cells were producing enterotoxin under varying nutritional and environmental conditions: NaCl concentration, culture aeration, and time and temperature of incubation in Brain Heart Infusion broth and shrimp slurries. At the various NaCl concentrations, the fluorescence of cells was found positively associated with enterotoxin B production only during the first 12 hr of growth. As the NaCl concentration was increased from 0 to 10%, the fluorescence of cells and toxin production decreased. Maximum for cell fluorescence and enterotoxin production was observed at 37 C. Little or no difference in cell fluorescence and enterotoxin production with both strains was found between Brain Heart Infusion broth and shrimp slurry cultures. All results obtained with the fluorescent-antibody technique were verified with double gel diffusion for enterotoxin detection and quantitation.


Production of fetal-like alkaline phosphatase by HeLa cells.


Activity of phenol oxidases at the puparium formation stage in development of nineteen lozenge mutants of Drosophila melanogaster.


Heterophile antigens in Ehrlich ascites fluid.


Immunological analysis of spores of Clostridium botulinum type E.


Lysis of bacterial spores with hydrogen peroxide.


Protease composition of a rennet substitute from Bacillus subtilis and properties of its component proteases.


Aging phenomena in spermatozoa. IV. Immunoserologic characterization of the deoxyribonucleic acid-protein extracted from fresh and aged bovine spermatozoa.


Isolation of milk leucocytes and their nuclei.


Baboon menstrual cycles affected by social environment.


A study of the menstrual cycle in Macaca irus with special reference to the detection of ovulation.


Rhythmic changes in the copulatory frequency of rhesus monkeys (Macaca mulatta) in relation to the menstrual cycle and a comparison with the human cycle.


Inactivation of prostaglandins by the lungs.


Relation of intracellular Ca2+ to retention of K+ by liver slices.


The replantation and transplantation of teeth. A review.


Neonatal separation: the maternal side of interactional deprivation.


A study of the danger of antibiotic preparation of the bowel for surgery.


Cesarean hysterectomy.

Cesarean hysterectomy.


Exercise-induced thrombocytosis.

Exercise-induced thrombocytosis.


Severe megaloblastic anemia due to nutritional vitamin B12 deficiency.


Electron microscopic study of the granules in guinea-pig bone marrow basophils.


Ultrastructural localization of alkaline phosphatase in human neutrophils.


Surgical complications of small bowel diverticula exclusive of Meckel's.


Temporary gastric partition: a model for staged repair of esophageal atresia with fistula.


The effect of hemorrhagic shock on the extracellular fluid space in the nephrectomized baboon.


Structural studies on Escherichia coli ribosomes. 3. Denaturation and sedimentation of ribosomal subunits unfolded in urea.


Turnover of protein and RNA of liver ribosomal components in normal and cortisol-treated rats.


Incorporation of N-formylmethionine into peptides by Pseudomonas aeruginosa extracts.


Valine transfer RNA. II. Kinetics of aminoacylation of the different species of valine transfer RNA in Bacillus subtilis.


Three cases of cyclopia.

Three cases of cyclopia.


The use of fluorescent antibody in the diagnosis of influenza.


Cryosurgery of primate pancreas.

Cryosurgery of primate pancreas.


Antigenicity of human prostatic acid phosphatase.


Immunological cross-reactivity between monkey and human thyroid stimulating hormone as determined by radioimmunoassay: a rapid procedure for purifying labeled 125I-TSH.


Effects of menstrual cycle and sex hormones on beta-glucuronidase and beta-acetylglucosaminidase activities in the "The Baboon Sex Skin".


Hormonal control of gestation in the intact rat.


Inhibition of endogenous growth hormone secretion by exogenous growth hormone infusion in the rhesus monkey.


Failure of high rates of glucocorticoid infusion to inhibit growth hormone secretion in the rhesus monkey.


Facial myology of theropithecus gelada, with special reference to specific facial gestures.


Insulinotropic and possible insulin-like effects of secretin and cholecystokinin-pancreozymin.


Thalamic relationships of the somatic sensory cortex in the rhesus monkey.


Identification of the thalamic nuclei on the basis of their cholinesterase content in the monkey.


The innervation of the upper urinary tract.


Release of TSH by oral administration of synthetic peptide derivatives with TRF activity.


Measurement of plasma cortisol in children and adults: a comparison of the double isotope derivative assay, competitive protein-binding analysis and the modified competitive protein-binding analysis.


Separation of thyroxine(T4)-binding proteins of human serum in polyacrylamide gel at pH 7.4. I. Effect of pH on distrubution of tracer quantities of T4.


Kinetics of N-ethylmaleimide inhibition of L-alanine-induced germination of Bacillus cereus spores.


Byrne WF: Absorption, excretion, and tissue distribution of orally and intravenously administered radiocerium as affected by EDTA.


Neurofibromatosis in childhood.

Neurofibromatosis in childhood.


Allergic bronchopulmonary aspergillosis in a child.


Toxicity of 3-acetyl pyridine in tuberculous and cord factor treated mice.


Comparative studies on alkylation of bacterial DNA in vivo and in vitro.


Interrupted hypothermia in experimental cerebral edema.


Regional cerebral blood flow measurement with small probes. II. Application of the method.


Suture materials in general surgery.

Suture materials in general surgery.


Stress response as a predictor of radiosensitivity in primates. SAM-TR-68-122.


Behavioral and circulatory responses to x-irradiation delivered at 200 rads per minute to whole body and trunk only. SAM-TR-68-111.


S-2-(3-aminopropylamino) ethyl phosphorothioic acid hydrate as a radioprotectant in rodents and primates. SAM-TR-68-120.


Indolylalkylamines as radioprotectants. SAM-TR-68-144.


Study of military blood banking and crossmatching using blood group antigens stored over five months in ACD-adenine (progress report). Rep No 816.


Rifampin: antibacterial activity in vitro and absorption and excretion in normal young men.


Studies on therapy of staphylococcal infections in monkeys. V. Comparison of cephalexin, oxacillin, cloxacillin, dicloxacillin and nafcillin.


Actions of purine analogs: enzyme specificity studies as a basis for interpretation and design.


Preliminary titration of the airway effects of a new bronchodilator aerosol (soterenol, MJ 1992) in humans with obstructive ventilatory disorders.


Clinical experience with a new antihypertensive agent, guanadrel sulfate.


Hemodynamic effects of a new antihypertensive agent, guanadrel sulfate.


Effect of timed release on the bioavailability of ascorbic acid: Ascorbicap vs. non-timed dosage forms.


Phenmetrazine vs. phenmetrazine with amobarbital for weight reduction: a double-blind study.


Double-blind and prolonged constant quinestrol therapy in the menopausal patient.


Needle-free injection of insect stings and bites.


A pilot study of AL-1021 in chronic schizophrenic patients.


Pilot study on the use of AL-1021 in the treatment of chronic schizophrenia.


AHR-1900: a butyrophenone derivative.

AHR-1900: a butyrophenone derivative.


A comparative trial of oxypertine and chlorpromazine in the treatment of acute psychoses.


Effect of barbiturate and electroanesthesia on some thalamothalamic and thalamocortical evoked potentials.


Hemodynamic alterations produced by intravenous diazepam in conscious, unrestrained dogs.


Clonidine (Catapres): a new antihypertensive agent.


An x-ray evaluation of the symptomatic response to Ru-Tuss tablets in sinusitis.


Peptic ulcer--a simplified ambulatory therapy.


Factors affecting the synthesis of penicillinase by Staphylococcus aureus.


Drug-plastic interactions. II. Sorption of p-hydroxybenzoic acid esters by capran polyamide and in vitro biological activity.


Physico-chemical properties of some vaccinial immunoprecipitinogens.


Endotoxin antibodies in patients on maintenance hemodialysis.


Effect of enhancer on growth cycle of ECHO virus 6 plaque mutant.


The effect of 5-iododeoxyuridine and actinomycin D on the multiplication of Junin virus.


Frequency and distribution of ABO and RHO(D) blood groups in Shiraz.


The propagation of "coronaviruses" in tissue-culture.


Treatment of angina pectoris with lidoflazine.


Polytene chromosomes and nucleic acid metabolism during macronuclear development in Euplotes.


Isolation of toxigenic strains of C. diphtheriae from skin lesions of children in a rural West Bengal community.


Effect of proteins on the reversibility of the thermal inactivation of Bacillus subtilis alpha-amylase.


Cytotoxicity of streptolysin "O" in tissue culture.


Autotrophic and heterotrophic metabolism of hydrogenomonas: regulation of autotrophic growth by organic substrates. The effects of a number of organic substrates on the autotrophic metabolism of Hydrogenomonas eutropha were examined. Dual substrate (mixotrophic) cultivation in the presence of hydrogen plus either fructose or alanine allowed autotrophic growth to begin immediately after the exhaustion of the organic substrate. On the other hand, the presence of acetate, pyruvate, or glutamate caused a lengthy lag to occur before autotrophic growth commenced. With acetate or pyruvate this lag (plateau) in the dicyclic growth curve was due to the repression of ribulose diphosphate carboxylase (RDPC) synthesis during mixotrophic growth. During heterotrophic growth with glutamate, RDPC was partially repressed; however, during mixotrophic growth, RDPC activity was high. Thus the delay of autotrophic growth was not due to a repression of RDPC by glutamate. The data suggest that glutamate interferes with autotrophic metabolism by repressing the incorporation of inorganic nitrogen. The repression of these vital autotrophic functions by acetate, pyruvate, and glutamate occurred both in the presence and absence of hydrogen, i.e., during both heterotrophic and mixotrophic cultivation. The derepression of the affected systems during the plateau phase of the dicyclic growth curves was demonstrated. Carbon dioxide assimilation by whole cells agreed well with the RDPC activity of extracts from cells grown under similar conditions.


Partial characterization of the factor responsible for tryptophanless death in Bacillus subtilis. The decline in colony-forming ability observed during tryptophan starvation of Bacillus subtilis auxotrophs is a concentration-dependent phenomenon. It does not manifest itself when the initial cell concentration is 10(6) cells/ml or lower. This property has been used to test the killing activity of different fractions of the dying cells. Most of the activity recovered is found in the supernatant fluid of the starved culture. Sensitive and resistant strains can be identified. Active supernatant fluids can only be isolated from tryptophan auxotrophs sensitive to tryptophanless death. Resistant cells neither produce nor respond to the factor, and sensitive cells respond only when deprived of tryptophan. The killing activity is continuously produced and released into the medium at least up to 4 hr after removal of tryptophan from the culture. The killing activity is deoxyribonuclease-, ribonuclease-, and heat-resistant.


Differentiation of two groups of Corynebacterium acnes. One hundred and forty-three strains of Corynebacterium acnes, isolated from human skin and acne lesions, were compared with three strains of Propionibacterium acnes from the American Type Culture Collection. The 146 organisms could be separated into two groups. Members of the larger group (129 strains) hydrolyzed gelatin and usually produced indole, Gel-In(+), but were unable to ferment trehalose, maltose, or sucrose, TMS(-). The deoxyribonucleic acid from selected strains of this group had an average guanosine + cytosine (GC) content of 60.5%. The members of the smaller group (17) were Gel-In(-), TMS(+), and the deoxyribonucleic acid had an average GC content of 63.9%. Studies with absorbed and unabsorbed antisera to the smaller group showed that although there were antigens shared by the two groups, it was possible to distinguish them serologically. Members of each group produced propionic acid. The principal fatty acid component of members of each group was iso-C(15) fatty acid. Seventy per cent of the Gel-In(+) strains were lysed by phage 174, whereas only one of 15 Gel-In(-) strains was lysed. Pending further information on the genetics of the two groups, those Gel-In(-), TMS(+) strains are tentatively designated C. acnes, group II.


Low-pH activation of Bacillus cereus spores. Heat activation of bacterial spores at low pH was investigated in detail. Unlike activation of spores in distilled water at a neutral pH, activation at low pH involves two superimposed processes: enhanced activation and death. Low-pH-activated spores failed to germinate in d-alanine, in contrast to spores activated at neutral pH, owing to the abolition of alanine-racemase activity. Morphological and permeability changes such as release and partial disruption of spores were dipicolinic acid-observed during low-pH activation. The kinetics pattern of low-pH activation, as well as the change in properties of the spores thereafter, suggest that the mechanism of low-pH activation differs from that of other kinds of heat-activation.


Beta-galactosidase: isolation of and antibodies to incomplete chains. A prematurely terminated polypeptide chain was purified to homogeneity from an Escherichia coli amber mutant strain containing the site of the mutation in the beta-galactosidase structural gene. The polypeptide was highly active against anti-beta-galactosidase, and had an amino acid composition similar to but not identical to that of beta-galactosidase. The molecular weight of the reduced, carboxymethylated chain in 6 m guanidine hydrochloride was found to be 89,000, in excellent agreement with the size predicted from the position of the mutation. This result adds further support to the conclusion that the gene specifies the structure of a single polypeptide chain. Antisera were prepared against partially purified preparations of this polypeptide and a similar one, of molecular weight about 100,000, produced by another amber mutant. These sera had lower titers towards beta-galactosidase than anti-beta-galactosidase. In the double-diffusion test, they reacted towards extracts of nonsense and deletion mutant strains in a pattern similar to that previously observed with anti-beta-galactosidase. A sensitive immunological test for cross-reacting protein was devised based on the inhibition by beta-galactosidase of the reaction between such protein and antibodies prepared against incomplete chains.


Increase in lytic activity in competent cells of Bacillus subtilis after uptake of deoxyribonucleic acid. Extractable lytic activity in competent cells of Bacillus subtilis 168 was markedly increased after treatment with homologous or heterologous deoxyribonucleic acid (DNA). This increase was prevented by deoxyribonuclease, and did not occur with B. subtilis W23 or with noncompetent B. subtilis 168 cells, neither of which take up DNA. Although the deoxyribonuclease-sensitive step in DNA uptake was completed within 10 min, the increase in lytic activity did not begin until more than 30 min after the addition of DNA. The increase was prevented by any of several antibiotics. These results are discussed in relation to the mechanisms for the uptake of transforming DNA and the lysis of transfected cells.


Lipid composition of Bacillus cereus during growth and sporulation. The lipid composition of Bacillus cereus during growth and sporulation was examined. The total lipid extract accounted for 2 to 3% of the dry weight of the cells and consisted of neutral lipids (30 to 40%) and phospholipids (60 to 70%). Phospholipids were separated by thin-layer chromatography into eight components; phosphatidyl ethanolamine, phosphatidyl glycerol, and diphosphatidyl glycerol were the major phospholipids and accounted for over 90% of the total. Also identified was a diglycosyl diglyceride and an alanine ester of phosphatidyl glycerol. Diphosphatidyl glycerol was more difficult to extract than the other components in vegetative and stationary-phase cells, but became increasingly easy to extract during spore maturation, and during sporulation cellular levels increased. Phosphatidyl glycerol had a high turnover rate; it accounted for about 70% of the phospholipid synthesis throughout sporulation but only represented between 30 and 40% of the total phospholipid at any time. Phosphatidyl ethanolamine, on the other hand, accounted for about 20% of the synthesis but was the major phospholipid (50 to 60% of the total).


Thermostable aldolase from Thermus aquaticus. Data are presented on the purification and properties of the thermostable fructose-1,6-diphosphate aldolase of Thermus aquaticus, a nonsporulating, extreme thermophile. The enzyme shows little activity at temperatures below 60 C and optimal activity at about 95 C. The enzyme was purified 43-fold by diethylaminoethyl cellulose column chromatography and Sephadex G-200 gel filtration. The enzyme is activated by high concentrations of NH(4) (+) and low concentrations of Fe(2+) and Co(2+) and is strongly inhibited by ethylenediaminetetraacetic acid (EDTA). The activation by Fe(2+) and Co(2+) and the inhibition by EDTA are both reversed by dialysis. The enzyme is greatly activated by cysteine and less so by other sulfhydryl compounds. Activation by cysteine is reversible by dialysis. The purified enzyme had a molecular weight as determined by Sephadex G-200 gel filtration of 140,000; after incubation of enzyme with cysteine, another molecular species was also found with a molecular weight of 70,000. The purified enzyme is stable at low protein concentrations to 97 C but is rapidly inactivated at 105 C. In cysteine the enzyme is more heat labile; heat inactivation in the presence of cysteine is prevented by substrate, although, in the absence of cysteine, substrate partially labilizes the enzyme to heat. The temperature optimum for enzyme activity is several degrees lower in the presence of cysteine than in its absence, and the K(m) is threefold lower. It is concluded that the T. aquaticus enzyme resembles some other aldolases of Rutter's class II, except for its extreme heat stability. The T. aquaticus enzyme is compared with that of Bacillus stearothermophilus, a moderate thermophile. Although the T. aquaticus enzyme is considerably more heat stable, the enzymes from the two thermophiles have many similarities. New data are presented which show that the B. stearothermophilus aldolase is metal ion-dependent, in disagreement with earlier reports.


Urea-mercaptoethanol-soluble protein from spores of Bacillus thuringiensis and other species. Treatment with urea-mercaptoethanol of purified spores of Bacillus thuringiensis, other Bacillus species, and Clostridium roseum solubilizes a protein fraction between 5 and 12% of the dry weight of the spores. This fraction behaves identically to the crystal protein of B. thuringiensis on acrylamide-gel electrophoresis. The protein from all of the Bacillus species shows partial homology with crystal protein, using the Ouchterlony immunodiffusion technique. A further fraction, similar in amount, can be removed from spores of B. thuringiensis by the addition of sodium lauryl sulfate to the urea-mercaptoethanol. Spores of B. thuringiensis extracted in these ways show no difference when compared to untreated spores with respect to viability or resistance to heat and ultraviolet-irradiation. The extracted spores do show differences in their germination requirements and their susceptibility to phase-darkening by lysozyme. It is concluded that an urea-mercaptoethanol-soluble protein or class of protein is a widespread component of bacterial spores, possibly located in the spore coat, and that this protein may be related to the crystal protein of B. thuringiensis.


Three years' observation of the teeth with pulpal involvement treated by corticosteroid antibiotic mixture.


Model for cell wall growth of Streptococcus faecalis. In exponentially growing and dividing cells of Streptococcus faecalis, it is proposed that the leading edge of the annularly closing cross wall is the point of extension for both cross wall and peripheral wall. Peripheral wall extension is thought to be produced by the separation or splitting of the cross wall at its junction with peripheral wall. This results in the pushing of the equatorial wall bands, found on S. faecalis walls, to subsequatorial positions. These bands therefore mark the separation of old wall from new wall. Mesosomal formation was observed usually to precede cross wall initiation.


Sources of blood for Triatoma dimidiata (Hemiptera: Reduviidae) in an endemic area of Chagas' disease in Costa Rica.


Hematozoa from Colorado birds. 3. Passeriformes.


Babesia spp. and Haemobartonella sp. in wild mammals trapped at the Agricultural Research Center, Beltsville, Maryland.


Sparganosis and "proliferative" spargana in vervets (Cercopithecus aethiops) and baboons (Papio sp.) from East Africa.


Wild reservoirs of Trypanosoma cruzi with special mention of the opossum, Didelphis marsupialis, and its role in the epidemiology of Chagas' disease in an endemic area of Costa Rica.


Further studies on mammalian susceptibility to experimental infection with Heterobilharzia americana.


Clinical nonspecificity of milk coproantibodies in diarrheal stools.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 12-1970.


Patient dosimetry in the electron treatment of large superficial lesions.


Technetium-99m DTPA: a new radiopharmaceutical for brain and kidney scanning.


Comparative aspects of folate and vitamin B12--metabolism in man and other primates.


Degeneration in the parvocellular portion of the lateral geniculate nucleus of the Cebus monkey. A light and electron microscope study.


Study of the frequency of blood group antigen and their combinations in dogs.


Evolution and heredity of blood groups in dogs.


Studies on blood transfusion in dogs.

Studies on blood transfusion in dogs.


The histology of myofibrillar hypoplasia of newborn pigs.


The chemical composition of neonatal piglet muscle and some observations on the biochemistry of myofibrillar hypoplasia occurring in otherwise normal litters.


Concerning the innervation of the kidney of the sheep.


Influence of chloramphenicol and ampicillin on antibody response in typhoid-paratyphoid fever.


Viral hepatitis.

Viral hepatitis.


The macrophage in tuberculosis.

The macrophage in tuberculosis.


Characterization of cytochrome c from Nitrobacter agilis.


Tyrosinase activity of the melanin and pteridine granules in goldfish chromatophores.


Stimulation of a protein glycosylation reaction by lysozyme.


The sulphatase of ox liver. 8. The action of carbonyl reagents on sulphatase A.


Task force on the cost of health services in Canada.


Immunology of conception and pregnancy.

Immunology of conception and pregnancy.


Splitting of human thyroglobulin. I. Reduction and alkylation.


Bile salt secretion and pool size in Rhesus monkeys with controlled interruption of the enterohepatic circulation.


A half-molecule GK plasmacytoma.

A half-molecule GK plasmacytoma.


Somatic variation and multiple neurofibromatosis.


Editorial. The mysteries of alveolar bone loss.


Case report. Bullet wound of the mandible.


Editorial. The dentist still has to go to court.


Etiology, cytology and diagnosis of oral cancer.


Since the days of Horace Hayden.

Since the days of Horace Hayden.


Comprehensive health planning in Southwestern Connecticut.


A brief review of dental enamel--recent findings.


Composite resins.

Composite resins.


Oral response to pin implants: First experimental evaluation.


The nurse's role in extending infection control to the community.


Symposium on nursing in long-term illness.


Biochemical genetics of bacterial sporulation. II. Membrane development suring sporulation of B. subtilis and its mutants.


Nursing the patient with a cerebrovascular accident.


The alcoholic patient with coexisting long-term illness. Nursing care.


Epilepsy.

Epilepsy.


Reduction in number of airborne bacteria by air cleaning devices in a closed space.


Effect of oral contraceptives on serum protein concentrations.


Serum protein concentrations in pregnancy. 3. Analysis of maternal-cord serum pairs.


Hyaline membrane disease.

Hyaline membrane disease.


A comparison of renin obtained from pregnant uterus and kidney of the dog.


Functional characteristics of renal urate transport in the Cebus monkey.


Enlarged pancreatic islets of tube-fed hypophysectomized rats bearing hypothalamic lesions.


Immunoglobulins in chronic urinary tract infections in children.


On intracellular parasites inducing hypertrophic and hyperplastic changes (xenoma) in the ectodermal epithelium of the chorioallantoic membranes in the chick embryo.


Studies on the effect of Corynebacterium diphtheriae and Corynebacterium pseudodiphtheriae on fetal guinea pig cell cultures.


Studies on the cytotoxic effect of Corynebacterium diphtheriae on fetal guinea pig cell cultures.


Inhibition of some enzymes by nonsteroid anti-inflammatory compounds at high temperature.


Identification of antibiotic WR 141.

Identification of antibiotic WR 141.


Glutamate, calcium ion-chelating agents and the sodium and potassium ion contents of tissues from the brain. 1. Salts of l-glutamate added to cerebral tissues maintained in glucose-saline-bicarbonate solutions cause the Na(+) content of the tissues to increase rapidly and K(+) to be lost. Entry of (22)Na(+) also is accelerated by l-glutamate and this acceleration is inhibited by low concentrations of tetrodotoxin. 2. Tissue Na(+) content and its rate of increase after the addition of l-glutamate are affected by the Ca(2+) of incubation media. 3. Very rapid and extensive entry of Na(+) to the tissue is caused by EDTA, and a moderate entry by citrate and ATP. Calculations of the concentration of free Ca(2+) in media after these additions indicate that Na(+) entry is sometimes associated with low Ca(2+) concentration, but that other substances, especially l-glutamate, act without greatly diminishing Ca(2+) concentration. 4. Experiments with 2,4-dinitrophenol and valinomycin are also reported and aspects of the Na(+) entry formulated and discussed.


Between-bottle variation as source of error in quality control sera.


Active accumulation of tetracycline by Escherichia coli. 1. At low concentrations of tetracycline (10mug/ml) net accumulation of the drug by Escherichia coli cells ceased after 7-10min. 2. At higher concentrations of tetracycline (>30mug/ml) the period of net accumulation of the drug was significantly extended. 3. The efflux of tetracycline from E. coli cells transferred from medium containing 10mug of tetracycline/ml to drug-free medium was a rapid temperature-dependent process and was accelerated by 2,4-dinitrophenol. 4. As the concentration of tetracycline in the preloading phase was increased, the rate of subsequent efflux of the drug progressively declined. The efflux of drug from cells preloaded in medium containing 200mug of tetracycline/ml was negligible, although efflux was readily provoked by 2,4-dinitrophenol, by N-ethylmaleimide or by omission of glucose from the medium. 5. The initial rate of uptake of tetracycline by E. coli cells was linearly proportional to the concentration of tetracycline in the medium up to the maximum concentration of drug obtainable under the experimental conditions used (400mug/ml, 0.83mm). 6. Although N-ethylmaleimide strongly inhibited the accumulation of tetracycline by E. coli, no evidence was obtained for the direct involvement of thiol groups in the transport process. It was concluded that N-ethylmaleimide inhibited accumulation by interruption of the energy supply of the cells. 7. Osmotic shock of E. coli cells did not significantly affect the influx of tetracycline, but promoted both efflux of tetracycline and cell lysis in cells treated with a high concentration of tetracycline. 8. A study of the distribution of tetracycline among the subcellular fractions of penicillin-induced spheroplasts preincubated with various concentrations of tetracycline indicated that 60-70% of the accumulated tetracycline was in the high-speed supernatant fraction. Sephadex chromatography showed that the tetracycline of this fraction was present as the free drug. Sephadex chromatography of a detergent extract of the membrane fraction, however, indicated that a significant proportion of the tetracycline radioactivity of this fraction was apparently bound to some macromolecular component. 9. Cellulose phosphate paper chromatography of cold-acid extracts of spheroplasts preloaded with tetracycline indicated that the accumulated drug was chemically unchanged. 10. Membrane preparations isolated from osmotically lysed penicillin-induced spheroplasts showed a temperature-dependent binding of tetracycline that was not energy-dependent and was not inhibited by N-ethylmaleimide. The binding process was stimulated by omitting Mg(2+) from the medium, but conversely was profoundly inhibited by EDTA. 11. The relevance of these findings to the probable mechanism of active tetracycline accumulation by E. coli is discussed.


Infectious arthritis in cattle caused by Corynebacterium pyogenes.


Changing perspectives and cross currents in nursing home care, 1968-1969.


Salmonella suppression by known populations of bacteria in flies. Survivorship of Salmonella typhimurium, Streptococcus faecalis, Proteus mirabilis, and Pseudomonas aeruginosa was studied in dibiotic and tribiotic interactions in vitro and in various regions of the digestive tract of the blow fly, Calliphora vicina. In dibiotic interactions, Salmonella typhimurium dominated Streptococcus faecalis and was dominated by P. mirabilis, but in neither case was it eliminated from the larval gut. In tribiotic interactions, there was synergic suppression and a definite trend toward elimination of Salmonella typhimurium from the gut. This trend approaches but does not match the total exclusion of S. typhimurium from the gut of maggots with a normal flora. Bacterial survival in the gut of the fly is discussed in relation to doubling time, sweep-out rate of the maggot and prepupal gut, and the midgut bactericide.


Regulation of chromosome replication in Bacillus subtilis: effects of amino acid starvation in strain 168. Regulation of chromosome replication in Bacillus subtilis strain 168, in response to starvation for an essential amino acid, was found to differ from that reported for Escherichia coli. Not all replication points stop at the terminus during amino acid starvation. There is some evidence, however, to indicate that preferred stopping sites might exist. Initiation at the origin can occur in the absence of total protein synthesis as well as when the deoxyribonucleic acid (DNA)- mass ratio is unbalanced. DNA synthesis appears to be controlled independently of the initiation event by a second regulatory circuit, that may utilize the DNA-mass ratio. Once initiated, chromosome replication does not always go to completion in an uninterrupted sequence.


Growth and sporulation of Bacillus subtilis mutants blocked in the pyruvate dehydrogenase complex. Two "ACE" mutants of Bacillus subtilis which require acetate for growth on glucose minimal medium have been isolated. They do not grow with acetoin, 2,3-butanediol, fatty acids, isoleucine, lipoic acid, malic acid, pyruvic acid, succinic acid, thiamine, or valine, but respond somewhat to glutamate or citrate. The mutants lack the activity of the pyruvate dehydrogenase complex; they excrete pyruvate and later acetoin. They grow in nutrient sporulation medium (NSMP) to one-half the normal turbidity and do not sporulate subsequently. When acetate is added to NSMP (at the optimal concentration of 0.07 m), the ACE mutants grow to the normal turbidity and then sporulate normally. Growth but not sporulation is restored in NSMP upon addition of 2,3-butanediol, citrate, glucose, glutamate, glycerol, or ribose, but not upon addition of acetoin, malate, oxaloacetate, pyruvate, and several other compounds. After growth in NSMP has stopped, the mutants incorporate uracil only at a very low rate, which can be increased by the addition of acetate, citrate, or glutamate. Furthermore, the metabolism of acetoin is prevented after growth has stopped but can be restored by the addition of acetate. All these results can be explained by a lack of reduced nicotinamide adenine dinucleotide (NADH) resulting from the deficiency in acetylcoenzyme A. In fact, after growth of the ACE mutants had stopped, the NADH concentration was at the borderline of measurability, whereas it increased significantly upon addition of glucose. The growing standard strain contains, at the same bacterial turbidity, at least 20 times more NADH (230 pmole/optical density unit at 600 nm) than the nongrowing ACE mutants. The isolated spores, obtained after growth in NSMP plus acetate, can be initiated to germinate in the presence of either l-alanine or the combination of l-asparagine, fructose, glucose, and potassium; addition of acetate is not required and has no effect.


Origin and sequence of chromosome replication in Neisseria meningitidis: influence of a genetic factor determining competence. Chromosome replication in Neisseria meningitidis was examined by enumerating mutants induced by nitrosoguanidine during synchronous replication after release from prolonged chloramphenicol inhibition. Clear maxima of mutagenesis were observed at certain times. These times were different for seven individual markers. At a definite time after the first maximum, there was a second one. The intervals between successive maxima were identical for all markers in each experiment. This permitted the construction of replication maps which were in general agreement with mapping based on marker frequency analysis by use of a transformation system. Comparison of replication in genetically competent and genetically incompetent variants substantiated the previous assumption that the change from genetic competence to genetic incompetence in this strain results in a change in the replication origin as well as in the direction of replication.


Adaptation of meningococcal L-forms to agar-free liquid medium. Neisseria meningitidis L-forms were adapted to an agar-free liquid medium after one passage in 0.05% agar-broth followed by two passes in 0.01% agar-broth.


Peptide synthesis by extracts from Bacillus subtilis spores. Cell-free peptide synthesis by extracts from vegetative cells and spores of Bacillus subtilis was analyzed and compared. The initial rate of phenylalanine incorporation in a polyuridylate-directed system was found to be in a similar range for the two extracts. However, spore extracts frequently incorporated less total phenylalanine as did the vegetative cell system. Optimal conditions for amino acid incorporation by spore extracts were found to be similar to those of vegetative cell extracts. Polyphenylalanine synthesis was stimulated by preincubation of both extracts prior to the addition of polyuridylic acid (poly U) and labeled phenylalanine. Both systems showed a dependence on an energy-generating system and were inhibited by chloramphenicol and puromycin. Ribonuclease, but not deoxyribonuclease, inhibited the reaction significantly. The presence of methionine transfer ribonucleic acid (tRNA(F)) and methionyl-tRNA(F) transformylase was demonstrated in spore extracts. An analysis of several aminoacyl-tRNAs in spores revealed that the relative amounts of these tRNAs were similar to those found in vegetative cells. Only lysine tRNA was found to be present in relatively greater amounts in spores. These results indicate that dormant spores of B. subtilis contain the machinery for the translation of genetic information.


Plaque assay system for several species of Rickettsia. The plaque assay developed in this laboratory for the Bitter Root strain of Rickettsia rickettsii has recently been shown to be appropriate for other rickettsiae.


Serological specificity of yeast mannan.


Inducible sucrase activity in Bacillus subtilis distinct from Levan-sucrase.


In vitro susceptibility of gram-negative bacilli to gentamicin.


The value of placental localization prior to amniocentesis.


Thyroxine-binding globulin values as a measure of placental adequacy.


An epidemic of neonatal diarrhoea in association with positive cultures of Corynebacterium diphtheriae.


The effect of experimental malaria on intracellular concentrations of sodium and potassium in muscle and liver.


Fatty acid composition of brain cholesterol esters in some neurological disorders with myelin involvement. (Preliminary communication).


Production of gonadotropin antibodies in rhesus monkeys. (Macaca mulatta).


Identification of respiratory syncytial virus antigens by agar gel diffusion and immunoelectrophoresis.


A survey of high-energy radiotherapeutic machines in Japan.


Endogenous neutralization of virus during fatal hog cholera illness.


Uterine blood flow and oxygen uptake during maternal hyperventilation in monkeys at cesarean section.


Genetics of esterases in man.

Genetics of esterases in man.


Purification and properties of N-acetyltransferase from mammalian liver.


Therapy of prolonged apnea after suxamthonium with purified pseudocholinesterase. New data on kinetics of the hydrolysis of succinyldicholine and succinylmonocholine and further data on N-acetyltransferase-polymorphism.


Pharmacogenetics in the detection of the hypersusceptible worker.


Yellow fever virus. II. Factors affecting the plaque neutralization test. In studies on the factors and conditions influencing the yellow fever (YF) virus plaque neutralization test, 60 min was found to be the minimal time necessary for equilibration of the virus-antibody complex at 23 and 37 C. Maximal virus titers in the diluent controls and the pre- and postinoculation serum-containing mixtures occurred by the 60-min adsorption time. Serum neutralization indices also seemed to level by this time. Heating (56 C for 30 min) decreased the neutralizing capacity of serum. However, the slope of the neutralization curve was not affected. The addition of native (unheated) "serum factor" in the form of fresh guinea pig or monkey sera partially restored the neutralizing activity lost by heating in some, but not all, sera. Many sera contained nonspecific inhibitors of YF virus infectivity and neutralization. Preliminary studies with ether extraction suggest that these inhibitors are lipid in nature.


Epidemiological fingerprinting of Pseudomonas aeruginosa by the production of and sensitivity of pyocin and bacteriophage. A new method has been devised to trace cross-infection by Pseudomonas aeruginosa. Unknown strains growing logarithmically in liquid media were treated with mitomycin C to induce the liberation of pyocin and phage. The lysates were then tested against 27 selected indicator strains, and the zones of clearing were differentiated as to killing by pyocin or lysis by phage. Twenty-four standard pyocin-phage lysates were then applied to each of the unknowns, and the sensitivity pattern was recorded. Thus, an "epidemiological fingerprint" consisting of 51 operational characteristics was established for each isolate. Organisms from the same source had identical or similar fingerprints, but organisms from different origins could easily be distinguished. Pyocin production, pyocin sensitivity, and phage production were found to be stable genetic characters; however, spontaneous mutations in phage sensitivity were frequently encountered. The epidemiological fingerprint has proven to be a sensitive tool in establishing the identity or dissimilarity of unknown strains. This method has been of great value in tracing the epidemiology of P. aeruginosa in the hospital environment. Each of the 157 P. aeruginosa strains tested has been typable by this method.


Standardized viral hemagglutination and hemagglutination-inhibition tests. I. Standardization of erythrocyte suspensions. A spectrophotometric procedure for standardizing red blood cell suspensions for use in viral hemagglutination tests is described. The procedure is based on highly reproducible cyanmethemoglobin absorbance readings at 540 nm on any suitable spectrophotometer. Target values for milligrams of cyanmethemoglobin per 100 ml are given for the red cell suspensions of all mammalian and avian species employed in viral hemagglutination tests. By use of these values and a cyanmethemoglobin standard curve for a particular photometer, approximate 4% erythrocyte suspensions can be diluted to any lesser concentration. Coefficients of variation for the various diluted suspensions range from 4 to 7%.


Standardized viral hemagglutination and hemagglutination-inhibition tests. II. Description and statistical evaluation. Standardized hemagglutination and hemagglutination-inhibition procedures are described and statistically evaluated for all animal viruses where applicable, except for rubella and the arbovirus group. The standardized tests employ a constant phosphate-buffered saline diluent and constant volumes of serum, antigen, and standardized erythrocyte suspension. The standardized hemagglutination test has a reproducibility of 84 to 96% with adenoviruses, rubeola, and the myxoviruses, and 78 to 93% with reoviruses; the standardized hemagglutination-inhibition test has a reproducibility of 95 to 100% with all viruses tested.


Simplified method for the production of carbohydrate antigen from Neisseria meningitidis. The Gotschlich procedure for the production of carbohydrate antigens from the supernatant fluids of cultures of Neisseria meningitidis groups A and C was modified. Steps involving digestion with nucleases and gel filtration through Sephadex G-50 were added, the number of extractions was greatly reduced, and high-speed centrifugations were eliminated. The antigens thus obtained appeared to be entirely comparable to those described by Gotschlich et al. in physical, chemical, and antigenic properties. A slight degree of protection of mice was repeatedly demonstrated with extraordinarily large doses of antigen (50 mug/mouse), but not with smaller doses. Toxicity of the antigens for laboratory animals was very slight.


Stability of St. Louis encephalitis virus in the airborne state. The aerosol stability of St. Louis encephalitis (SLE) virus was studied over a 6-hr period at a temperature of 21 C and relative humidity values of 23, 46, 60, and 80%. Aerosols were generated from and collected in 0.75% bovine albumin-buffered saline, and spores of Bacillus subtilis var. niger were used as the tracer to determine the physical decay of the aerosols. Aerosol samples were titrated in BHK-21 cell monolayers for surviving SLE virus. The results of this study indicated that, under the test conditions employed, relative humidity had no influence on the stability of SLE virus in the airborne state.


Comparison of methods for tuberculosis bacteriology. To improve efficiency of isolation of tubercle bacilli from clinical specimens, the following recommendations are presented. (i) Employ multiple specimens consisting of a combination of morning sputums for the early detection of positives, along with 24-hr sputum pools for the greatest total yield of positives. (ii) When timing is rigorously controlled, Zephiran-trisodium phosphate and sodium hydroxide-acetylcysteine are comparable, but if timing cannot rigidly be controlled, employ the Zephiran-trisodium phosphate digestion procedure to allow the greatest freedom in exposure time with the lowest kill rate to tubercle bacilli. (iii) Employ both an agar medium incubated in 5% CO(2), for the early detection of positives as well as positives in the presence of contaminants, and an egg medium, preferably with CO(2), to increase the yield of positives.


Incidence of mucoid Pseudomonas aeruginosa from clinical sources. The high frequency of pulmonary infection by mucoid strains of Pseudomonas aeruginosa in patients with cystic fibrosis can be interpreted to represent an apparent mutation resulting from environmental factors within the host.


A novel protoplast-bursting factor (surfactin) obtained from Bacillus subtilis IAM 1213. I. The effects of surfactin on bacillus megaterium KM.


A novel protoplast-bursting factor (surfactin) obtained from bacillus subtilis IAM 1213. II. The interaction of surfactin with bacterial membranes and lipids.


Effect of externally added ATP on glucose uptake by isolated rat soleus muscle.


Reversible effect of L-dopa on tremor and catatonia induced by alpha-methyl-p-tyrosine.


Pharyngeal diverticulum: case review and operative treatment.


Diabetogenic effects of streptozotocin in rhesus monkeys.


Cortical motor potential in monkeys before and after upper limb deafferentation.


Brain glycogen of the recovering asphyxiated monkey newborn.


Encephalopathia hypercalcemica. A clinical and electroencephalographic study in myeloma and other disorders.


Subacute sclerosing panencephalitis: isolation of a virus encephalitogenic for ferrets.


Subacute sclerosing panencephalitis.

Subacute sclerosing panencephalitis.


Effects of cerebral ischemia, seizures, and anoxia on arterial concentrations of catecholamines.


Immunochemical quantitation of beta2-glycoprotein I in various diseases.


Spherules in the serodiagnosis of coccidioidomycosis.


Synthesis of transferrin by human peripheral blood lymphocytes.


Studies on lipid peroxides in platelets. I. Method of assay and effect of storage.


A micromethod for the determination of serum thyroxine: studies of serum thyroxine concentrations and thyroxine binding in rat serum.


Inhibition of drug-metabolizing enzymes of liver microsomes by hydrazine derivatives in relation to their lipid solubility.


Temperature-sensitive mutants of Bacillus subtilis. I. Multiforked replication and sequential transfer of DNA by a temperature-sensitive mutant. A temperature-sensitive mutant of Bacillus subtilis 168 was isolated. Its chromosome was found to undergo multiforked replication at normal temperature. The mutant cells then transferred chromosomes to recipient cells sequentially from the origin to the terminus of the chromosome at sublethal temperatures. Sequential transfer of the chromosome facilitated the determination of the relative positions of markers on the chromosome. Linkage between origin (adenine-16) and terminus (methionine) was demonstrated.


Genetic mapping of antibiotic resistance in markers Bacillus subtilis. Several antibiotic resistance markers in Bacillus subtilis have been mapped by three-factor transduction crosses using bacteriophage PBS1. These markers, which are thought to be located in genes coding for ribosomal proteins, are within a segment comprising about 5 per cent of the B. subtilis genetic map, close to the major group of rRNA cistrons. The order of genetic makers in this region was found to be cysA14 mic-1 dagger str-1 (ery-1, ole-2) spc-2 bry-2 lin-2. The positions of neo-2, nea-1, and kan-2 are uncertain, although they are located in this region to the right of cysA14.


Hamster-tropic sarcomagenic and nonsarcomagenic viruses derived from hamster tumors induced by the Gross pseudotype of Moloney sarcoma virus. Hamster sarcomas induced by the Gross pseudotype of Moloney sarcoma virus yielded a virus sarcomagenic for hamsters but not mice. This virus was able to produce foci on hamster embryo cells, but not on mouse embryo cells. A hamster-tropic nonfocus-forming helper virus was also found in the viral stocks. These hamster-tropic viruses are not immunologically related to the murine viruses in the original inoculum but appear to represent indigenous C-type RNA viruses of the hamster.


Immunoglobulin M: pentameric Fcmu fragments released by trypsin at higher temperatures. Immunoglobulin M (IgM, 19S macroglobulin) is a high molecular weight antibody molecule currently thought to be composed of five identical 7S subunits linked by disulfide bonds, perhaps in a cyclic conformation. Reported molecular weights of IgM vary from 8 x 10(5) to 10(6). Proteolytic cleavage of this molecule under standard conditions with several enzymes results in the formation of ten antigen-binding (Fab) fragments per mole, each Fab having a molecular weight of 40,000. The remainder of the molecule, designated Fc by analogy with the structure of immunoglobulin G (IgG), is highly susceptible to further enzyme cleavage and breaks down to dialyzable peptides. However, under carefully controlled conditions, an intact Fc-like fragment in low yield can be isolated from short-term papain digests of IgM at 37 degrees C. Availability of the Fc portion of the molecule is important not only for structural studies but because it may determine certain important biological properties of the molecule such as complement fixation and placental permeability. We here report that trypsin cleavage of IgM at temperatures exceeding 50 degrees C results in the production of excellent yields of an intact Fc fragment, whereas no detectable Fc fragment is produced by trypsin cleavage at 37 degrees C. Fab fragments are obtained under both temperature conditions, and an Fab dimer has been identified in high temperature digests. This unusual difference in the products of enzyme digestion with temperature is unexplained but may be related to steric changes induced in the IgM molecule by heat. Fc fragment isolated from high temperature digests contains two-thirds of the carbohydrate of the intact molecule, and has a molecular weight of 342,000. The molecular weight falls to 67,300 after treatment with disulfide reducing agents, thus supporting the concept of a pentameric structure for IgM.


Village and tribal genetic distances among American Indians, and the possible implications for human evolution. Pair-wise genetic distances based on six genetic systems (Rh, MNSs, Kidd, Duffy, Diego, and haptoglobins) are presented for seven villages of Makiritare Indians, seven villages of Yanomama Indians, and 12 Indian tribes of Central and South America. It is shown that the mean genetic distance between Indian villages is 85-90 per cent of the distance between tribes. Since in the past, the initial event in the formation of a new tribe was probably the breaking away of one or several related villages from an established tribe, it is clear that this initial event could have profound consequences for subsequent tribal gene frequencies. By the criterion of changes in gene frequency in polymorphic genetic systems, the maximal rate of evolution in the American Indian has been approximately 100 times more rapid than the mean rate suggested by calculations based on amino acid substitutions in certain polypeptides of a variety of organisms. The above-mentioned findings could account in part for this 100-fold difference. Some other factors which might diminish the apparent difference between the results of these two types of calculations are mentioned.


Ribosomal RNA homologies among distantly related organisms. The similarity in base sequences of ribosomal RNA (rRNA) in widely divergent organisms was compared. Hybrids were prepared between Escherichia coli and pea rRNA on the one hand and the DNA of several organisms on the other. The varying thermal stabilities of these hybrids were taken as evidence for a hierarchy of rRNA relatedness among some higher plants, and for phylogenetic relationships among some species of bacteria, protozoa, fungi, plants, and animals. The large rRNA molecule appears to have evolved faster than the small rRNA molecule.


A specific complex between a restriction endonuclease and its DNA substrate. In the presence of Mg(++), ATP, and S-adenosylmethionine, the DNA restriction endonuclease R.K forms a specific complex with its DNA substrate. The complex can be detected by its retention on nitrocellulose membranes.


Nuclear magnetic resonance studies of the structure and binding sites of enzymes. XI. Characterization of selectively deuterated analogs of staphylococcal nuclease. The preparation of five different selectively deuterated analogs of the enzyme staphylococcal nuclease has been previously reported.(1-3) The five selectively deuterated enzymes have full enzymatic activity with DNA and RNA substrates and identical amino acid compositions; and they all appear to have similar conformations in solution, despite their very different hydrogen/deuterium contents.


Pseudomonads and man.

Pseudomonads and man.


The carrier protein in contact sensitivity.


Detection of biologic changes in animals exposed to low levels of ionizing radiation. SAM-TR-69-41.


Evolutionary systematics of the chimpanzee: immunodiffusion computer approach. ARL-TR-69-10.


Experimental impact protection with advanced restraint systems: preliminary primate tests with air bag and inertia reel-inverted-Y yoke torso harness. AM 69-4.


Seat belt injuries in impact. AM 69-5.

Seat belt injuries in impact. AM 69-5.


Pathology of trauma attributed to restraint systems in crash impacts. AM 69-3.


Hereditary multiple ocular anomalies in Australian shepherd dogs. (preliminary report).


Effect of changes in potassium and calcium concentrations on diastolic threshold and strength-interval relationships of the human heart.


The use of blood groups in the study of populations.


The effect of isoantibodies on red-cell survival.


The use of apes and monkeys for the investigation of the human blood groups.


Heterogeneity and structure of antibody-combining sites.


Practical physiological schema for the identification of Herellea vaginicola and its differentiation from similar organisms. A seven-test schema is presented that will identify Herellea vaginicola and differentiate it from Mima, Moraxella, and other gram-negative organisms whose biochemical reactions are similar to those of Herellea. Of 133 cultures examined, 49 were Herellea strains accurately identified by this schema: 34 of the 49 cultures were the capsular type strains of Herellea and 15 strains were from a group of 53 ATCC cultures listed under several different taxons. The remaining 84 non-Herellea cultures yielded 4 (4.7%) false positive identifications for Herellea; two were from a selected group of organisms (EO-1) whose taxonomic position is undetermined and two were Klebsiella ozaenae. Since neither of these groups is frequently encountered in clinical specimens, from a practical viewpoint they present no particular problem in the identification of Herellea. We recommend the use of the seven tests as described in this paper as a routine procedure for the identification of H. vaginicola.


Therapy of staphylococcal infections in monkeys. IV. Further comparison of triacetyloleandomycin and erythromycins. Intravenous inoculation of a penicillin-resistant, phage type 80/81 staphylococcus caused lethal infection in 8 of 15 untreated monkeys. Daily intragastric administration of 50 mg/kg of triacetyloleandomycin, erythromycin estolate, and erythromycin ethylsuccinate was followed by mortalities of 0 of 16, 3 of 16, and 3 of 10, respectively. At dose levels of 25 and 12.5 mg/kg, none of 7 and 4 of 7 receiving triacetyloleandomycin and erythromycin estolate, respectively, died, as compared to 3 of 4 deaths in controls. In vitro sensitivity data and serum antibacterial levels would suggest that triacetyloleandomycin would be the least effective therapeutically. However, this prediction was not fulfilled in these studies of experimental infections in monkeys wherein triacetyloleandomycin was a very effective antimicrobial agent.


Sulfadiazine susceptibility patterns of Neissera meningitidis group Y. Four of 46 Neisseria meningitidis group Y strains (8.6%) were resistant to 1.0 mg of sulfadiazine per 100 ml when tested by the agar plate-dilution technique.


Osseous metaplasia in the human mammary gland.


Enzymatic and ultrastructural evaluation of hepatic preservation in primates.


Intraperitoneal insemination of Macaca mulatta.


Perspectives for BCG standardization.

Perspectives for BCG standardization.


Development of ovulatory refractoriness in the rabbit to cyclic injections of human chorionic gonadotropin.


A comparison of autologous and allogeneic tooth transplants in rhesus monkeys.


Revascularization and bone healing after anterior mandibular osteotomy.


Immunogenicity of 1967 polyvalent and 1968 Hong Kong influenza vaccines.


Samuel T. Darling and human sarcosporidiosis or toxoplasmosis in Panama.


Utilization of general practitioners for drug evaluation.


Rare complications with intravenous hyperosmotic alimentation.


Anti-Rh activity of human gamma G4 proteins.


Ocular complications of chlorpromazine therapy.


Review of mental hygiene and related legislation for the year 1968.


A model in comparative teratogenesis. II. Response to 5-fluoro-2-deoxycytidine at successive stages in organogenesis of mice of strains C57BL-6JHanFfm and C57BL-10JFm- + ld.


A study of the effects of sympathetic stimulation on cerebrovascular hemodynamics using the dye dilution technique.


Paraproteinemia in a child with leukemia.


Prevalence of precipitating antibody to antigens derived from Burkitt lymphoma cultures infected with herpes-type virus (EB virus).


Electrocardiographic studies in rhesus monkeys.


The action of serotonin (5-hydroxytryptamine) in vivo upon the microfilariae of Dirofilaria, Loa and five other species.


Effects of very soft x-rays on bacterial spores. NYO-2740-6.


The changing epidemiology of septic arthritis in children.


Ten-year functional nonunion of a tibial fracture. A case report.


Dosimetry studies for a fast neutron therapy beam. NYO-2740-6.


Percutaneous arthrodesis of the ankle joint.


Radiobiology. NYO-2740-6.

Radiobiology. NYO-2740-6.


An evaluation of deoxycytidinuria in the subhuman primate as a radiation biodosimeter. LA-4223.


Bifid os calcis. An anomalous ossification of the calcaneus.


Management of open wounds of joints during the Vietnam war. A preliminary study.


Attempt to clone marrow cells in spleens of lethally irradiated rhesus monkeys. SAM-TR-69-74.


Leukocyte typing in the rhesus monkey. SAM-TR-69-45.


Cerebro-hepato-renal syndrome of Zellweger.


Isolation of Corynebacterium diphtheriae in the environment of skin carriers.


Viral hemorrhagic encephalopathy of rats. II. Pathogenesis of central nervous system lesions.


Reactions of human endometrium to the intrauterine device. I. Correlation of the endometrial histology with the bacterial environment of the uterus following short-term insertion of the IUD.


Clinical significance of postirradiation dysplasia of the uterine cervix.


Human paleopathology and some diseases in living primitive societies: a review of the recent literature.


Endotoxin-containing preparations of Brucella abortus: chemical studies.


Blood groups, red cell enzymes, and cerumen types of the Ahousat (Nootka) Indians.


Endotoxin-containing preparations of Brucella abortus: biological studies.


Hematologic values of the Panamanian marmoset (Saguinus geoffroyi).


Nature of love--simplified.

Nature of love--simplified.


Histological study of mediastinal lymph nodes in clinical sarcoidosis. A report of 86 cases.


Effects of critical stenosis of the carotid artery and compromised cephalic blood flow.


Preparation of a purified adenovirus diagnostic antigen by gel filtration.


Properties of a Pseudomonas aeruginosa bacteriophage phi-MC.


Genetics of Pseudomonas.

Genetics of Pseudomonas.


Antimicrobial effects of anacardiac acids.


Recurrent hepatomegaly and transient alteration of liver functions in an adolescent with diabetic acidosis.


Metabolic changes in infectious diseases.


Control of postoperative pain: a comparison of the efficacy and safety of pentazocine, methotrimeprazine, meperidine, and a placebo.


A double-blind evaluation of the therapeutic efficacy of salutensin versus hydropres-50 in essential hypertension.


Therapeutic efficacy of bufexamac in rheumatoid arthritis: a double-blind comparison with indomethacin.


Controlled trials of an amitriptyline-fluphenazine combination in depressive neuroses and psychoses: a collaborative study.


Some experimental studies with dimethyl sulfoxide (DMSO) in cold injury.


Trypanosoma cruzi: thyroid hyperplasia, hyperthyroidism and variance in thyroid function in mice.


Menstruation changes as unusual side effect in a molindone trial.


Comparative studies of phenol oxidase activity during pupal development of three lozenge mutants (lz8,lz,lzk) of Drosophila melanogaster.


Recovery of the gonococcus from exudates dried on filter paper.


Inhibition of complement-mediated hemolysis by rheumatoid arthritis sera studies in agarose gel.


The use of ionization chambers for the absorbed dose calibration of high energy electron beam therapy units.


Corneal calcification in spontaneously daibetic mice.


Studies on the mode of action of some cephalosporin derivatives.


Calcium efflux from frog twitch muscle fibers. An apparatus is described which collects the effluent from the center 0.7 cm of a single muscle fiber or bundle of muscle fibers. It was used to study the efflux of (45)Ca from twitch muscle fibers. The efflux can be described by three time constants 18 +/- 2 min, 300 +/- 40 min, and 882 +/- 172 min. These kinetics have been interpreted as those of a three-compartment system. The fastest is thought to be on the surface membrane of the muscle and of the T system. It contains 0.07 +/- 0.03 mM Ca/liter of fiber and the Ca efflux is 0.11 +/- 0.04 pM Ca/cm(2). sec. The intermediate rate compartment is thought to represent the Ca in the longitudinal reticulum. It contains approximately 0.77 mM Ca/liter. Only the efflux from this compartment increases during stimulation. The most slowly exchanging compartment is poorly defined. Neither Ca-free nor Ni-Ringer solutions alter the rate of loss from the fastest exchanging compartment. Ni apparently alters the rate of loss from the slowest compartment.


Serogroups of Pseudomonas aeruginosa and the immune response of patients with cystic fibrosis.


Effect of bacteriocin from Streptococcus faecalis on microbial L-forms.


Serologic aspects of sporotrichosis.

Serologic aspects of sporotrichosis.


The assessment and treatment of drug-resistance problems in tuberculosis.


Symposium on drug resistant tuberculosis. Clinical drug resistance in Ireland 1964-68.


Rifampicin and ethambutol in the treatment of drug resistant and far advanced pulmonary tuberculosis (with a note on capreomycin).


Symposium on drug resistant tuberculosis. Reserve drug combinations.


How high a priority is health care?

How high a priority is health care?


Heterotopic pulmonary ossification.

Heterotopic pulmonary ossification.


The health care dilemma: new directions.


Laryngotracheal-esophageal cleft.

Laryngotracheal-esophageal cleft.


Responses to electrical and reflex autonomic stimulation in dogs with cardiac transplantation before and after reinnervation.


Retrograde pulmonary venous pressure measurement--fact or artifact?


Pulmonary changes following hemorrhagic shock and resuscitation in baboons.


Cell movement and cell spreading: a passive or an active process?


Three types of virus particle in Penicillium stoloniferum.


Allotype polymorphism of low density beta-lipoproteins in pig serum (LDLpp I, LDLpp 2).


Petit mal--a critical review.

Petit mal--a critical review.


Transverse fracture of the proximal humeral articular cartilage in dogs. (So-called osteochondritis dissecans).


Vitamin E and the hematopoietic system.

Vitamin E and the hematopoietic system.


An in vitro test of the immunosuppressive activity of antilymphocyte serum.


Research in health education: report of a WHO Scientific Group.


Immunologic aspects of plasminogen.

Immunologic aspects of plasminogen.


Microbiological aspects of ethylene oxide sterization. I. Experimental apparatus and methods. A specially built thermochemical death-rate apparatus is described which can be used to determine the resistance of microorganisms to ethylene oxide under controlled conditions. The apparatus was designed to provide instantaneous exposure of microorganisms to ethylene oxide and to eliminate variables that could result in errors when death kinetic reaction rates are calculated. The apparatus is used to obtain ethylene oxide resistance data which are useful in evaluating and developing sterilizing cycles for materials with known bacterial concentrations, as well as for calculating probability factors on which a given test condition can be expected to provide sterilization.


Microbiological aspects of ethylene oxide sterilization. 3. Effects of humidity and water activity on the sporicidal activity of ethylene oxide. An investigation determined the effects of environmental moisture content or water activity (Aw), exposure humidity, and sterilant concentration on the resistance of microbial spores. Decimal reduction values [expressed as D values at 54.4 C-specified concentration (milligrams per liter) of ethylene oxide] were determined from spore destruction curves of Bacillus subtilis var. niger dried on hygroscopic and nonhygroscopic surfaces. Four groups of spore preparations were preconditioned in one of four Aw environments (<0.1, 0.1, 0.5, 0.95) for 2 weeks or longer and were exposed to 500 mg of ethylene oxide per liter at 54.4 +/- 3 C and 10, 50, and 95% relative humidity in a specially designed thermochemical death rate apparatus. A fifth group did not receive any preconditioning treatment and was exposed immediately after preparation, in the same apparatus at the same temperature, to ethylene oxide concentrations of 200, 400, 600, 800, and 1,200 mg/liter and relative humidities of 15, 30, 50, 60, and 90%. The resistance of the spores on both types of surfaces to ethylene oxide increased proportionately with the Aw of the conditioning environment. The study also showed that moisture in the exposure system was not as critical a variable as the ethylene oxide concentration. The spore destruction rates, irrespective of the carrier types at all concentrations and at different humidities, varied little from one another. The decimal reduction values were reduced as the ethylene oxide concentration increased, and no optimal exposure humidity concentration was observed.


Physiologic study of the uterine venous drainage of the pregnant rhesus monkeys.