Antibiotic sensitivity of gonococcal strains isolated in the South-East Asia and Western Pacific regions in 1961-68. A decreasing susceptibility of gonococcal strains to penicillin and other antibiotics has been observed in most parts of the world, including the WHO South-East Asia and Western Pacific regions. In the Neisseria Department, Statens Seruminstitut, Copenhagen, the antibiotic sensitivity of about 100 gonococcal strains from these regions has been determined. The strains were collected during 2 periods, one in 1961 (Ceylon and the Philippines), the other in 1967-68 (Thailand, Taiwan, Viet-Nam and Hong Kong).It was found that the incidence of gonococcal strains with decreased susceptibility to penicillin and most of the more generally used antibiotics is increasing in these regions. The distribution of the 50% inhibitory concentrations of penicillin, tetracycline, streptomycin and spiramycin was similar for the strains isolated in the Philippines in 1961 and for those from the other areas in 1967-68, namely, strains with "normal sensitivity" were lacking. Most of the strains collected in 1961 were fully sensitive to chloramphenicol and erythromycin, whereas about 25% of the strains collected in 1967-68 were only moderately sensitive to chloramphenicol, and about 75% were moderately sensitive to erythromycin; 1 strain was resistant to erythromycin. Only 10% of these strains were sensitive to all the antibiotics employed.In contrast to these findings, several authors report an increasing susceptibility of the gonococcal strains in northern Europe. Possible explanations for the reversion to "normal sensitivity" are discussed briefly.


Recent developments in the laboratory diagnosis of gonococcal infections. The author reviews the more important studies on the laboratory diagnosis of gonococcal infections published since 1964. The most significant new developments are the use (a) of fluorescent antibody techniques and (b) of selective media for the identification and isolation of Neisseria (N.) gonorrhoeae. The sensitivity and specificity of culture methods and of the direct "delayed" fluorescent antibody technique (FAT), both at their best, are very similar, but the FAT allows for more rapid reporting. The results of the direct "undelayed" FAT can be given even more rapidly, but, especially with specimens from women, it is less sensitive than culture or the direct "delayed" FAT. The use of a selective medium facilitates the recognition and identification of N. gonorrhoeae, but the advantage of a selective medium over a non-selective medium depends very much on the skill of the technical personnel involved. One disadvantage is the existence of gonococcal strains that are very sensitive to the antibiotics used hitherto for the suppression of Gram-positive contaminants. Ideally, selective medium should be used in combination with the direct "delayed" FAT after growth on non-selective medium. Other new achievements are a complete, chemically defined, protein-free liquid medium for the culture of fastidious Neisseriae; a commercially available chemically defined enrichment supplement; and a new specimen-transport kit using Stuart's medium.The author also reviews recent reports which suggest that the in vitro sensitivity of N. gonorrhoeae to penicillin and other antibiotics used for treating gonorrhoea is generally decreasing; however, some reports of increasing sensitivity have lately come from Scandinavia.


Deaths in ewes following the administration of copper calcium edetate for prevention of swayback.


The membrana limitans gliae superficialis in vertebrates.


Bovine testicular antihyaluronidase in rabbits. Production and purification.


Clinical observations on diverticulosis-diverticulitis of the appendix. Rectal bleeding in association with symptoms of acute appendicitis suggests diagnosis.


Primary macroglobulinemia.

Primary macroglobulinemia.


Retinal topography and the blue-arcs phenomenon.


The spectral sensitivity of evoked potentials from the retina and cortex of nocturnal and diurnal monkeys.


Rubella virus. Isolation, characterization, and laboratory diagnosis.


Review of studies with inactivated rubella virus.


Visual evoked responses in the brain injured monkey.


[A case of hyperosmotic diabetic coma withot acidosis].


Aspects of drug metabolism relating to pharmacologic activity.


Bone loss as a result of immobilization and chelation. Preliminary results in Macaca mulatta.


Ehlers-Danlos Syndrome with some unusual skeletal abnormalities.


Preliminary studies on the antigenicity of irradiated and nonirradiated peripheral nerve.


Synthesis of bacterial flagella. II. PBS1 transduction of flagella-specific markers in Bacillus subtilis. The linkage relationship of mutants involved in the synthesis of flagella was determined by PBSl transduction. Mutants that affect the structure of flagellin (hag) and temperature-sensitive mutants (flaTS) that produce flagella when grown at 37 C but not when grown at 46 C were examined. All of the mutants were found to be linked to the hisA1 marker. The flaTS mutants fell into three clusters. Group A contained the majority of mutants which were loosely grouped around the hag locus. Group B mutants were segregated from the hag locus and appeared closely linked to the phage adsorption site gene (gtaA), and group C was only loosely linked to hisA1 and thus far contains only one mutant. A flagella locus (ifm) affecting both the degree of motility and level of flagellation was shown to map near group A. Mutants affecting motility (mot) were not linked to hisA1 by PBSl transduction. Several markers previously shown to link to hisA1 were ordered with respect to hisA1 and the flagellar genes.


Organization of enzymes in the polyaromatic synthetic pathway: separability in bacteria. ULTRACENTRIFUGATION IN SUCROSE DENSITY GRADIENTS WAS EMPLOYED TO ESTIMATE THE MOLECULAR WEIGHTS AND TO DETERMINE POSSIBLE PHYSICAL AGGREGATION OF THE FIVE ENZYMES CATALYZING STEPS TWO TO SIX IN THE PRECHORISMIC ACID PORTION OF THE POLYAROMATIC SYNTHETIC PATHWAY IN SIX SPECIES OF BACTERIA: Escherichia coli, Salmonella typhimurium, Aerobacter aerogenes, Bacillus subtilis, Pseudomonas aeruginosa, and Streptomyces coelicolor. The five enzymes were not aggregated in extracts of any of the species examined, nor are the genes encoding these enzymes clustered in those bacterial species for which genetic evidence exists. (An initial examination of the blue-green alga Anabaena variabilis indicates nonaggregation in this species also.) This situation in bacteria is in marked contrast to that found in Neurospora crassa and other fungi in which the same five enzymes are associated as an aggregate encoded (at least in the case of N. crassa) by a cluster of five genes. In addition, also in contrast to N. crassa, no evidence was obtained for more than one kind of dehydroquinase activity in any of the bacteria examined. These comparative results are discussed in relation to the origin, evolution, and functional significance of the gene-enzyme relationships existing in the early steps of aromatic biosynthesis in bacteria and fungi.


Bacteria-shaped gymnoplasts (protoplasts) of Bacillus subtilis. Addition of glucose to the medium in which Bacillus subtilis was grown lowered the pH and increased the amount of lysylphosphatidylglycerol relative to the phosphatidylglycerol content of the membrane fraction. This change in phospholipid composition was accompanied by changes in the shape and behavior of the gymnoplasts obtained by cell wall removal with lysozyme. These gymnoplasts appeared to retain most of their original cell shape and internal organization, often with preservation of the mesosomes. Cells harvested from neutral growth medium gave the usual spherical gymnoplasts. In a hypotonic medium, the spherical gymnoplasts lysed rapidly, whereas the rod-like gymnoplasts lost only part of their cell content while showing a tendency to preserve the original shape. This type of gymnoplast could not be produced from cells grown in neutral medium by simply raising the magnesium concentration. When this was done the gymnoplasts assumed bizarre shapes; they became compact and susceptible to the tonicity of the medium. Gymnoplasts or protoplasts, produced from bacilli exposed to low pH values, were found not to conform to the formulations on "protoplasts" given in 1958 by 13 authors. Cells exposed to a low environmental pH during growth seemed to possess a more rigid membrane structure than cells grown at neutral pH.


Ammonium sulfate coprecipitation antibody determination with purified staphylococcal enterotoxins. The ammonium sulfate coprecipitation technique of Farr was applied in a study of the purified enterotoxins of Staphylococcus aureus. Ammonium sulfate coprecipitation of iodine-131-labeled enterotoxins A, B, and C, with the use of a 1.6 m concentration of (NH(4))(2)SO(4), revealed differences in the antigen-binding capacity of normal and immune rabbit sera for the enterotoxins. The coprecipitation technique provided a quantitative test for detecting antibody to enterotoxin that was more sensitive than agar-gel diffusion methods. Antigen-binding tests suggested the presence of similar antigenic determinant groups in all three toxins. Measurable antigenbinding capacities for enterotoxins A, B, and C were detected in sera of normal human subjects and became elevated in several subjects accidently exposed to enterotoxin.


Sequential entry of transforming markers into Neisseria meningitidis after chromosome alignment. The kinetics of appearance of transformants as a function of time of exposure to deoxyribonucleic acid (DNA) was examined in Neisseria meningitidis. Incubation with chloramphenicol for as long as 2 hr, which probably leads to chromosome alignment, resulted in augmentation of the lag period before the appearance of the first transformants. The lag periods thus found were dependent upon the marker tested. This permitted the construction of a time map according to the lag periods observed for individual markers. This map was in general agreement with the chromosome map of the recipient strain as determined by marker frequency analysis. Transformation of recipient cells with chromosomes aligned by growth to the stationary phase showed the same type of increased lag in the appearance of transformants before the logarithmic phase of growth had again been reached. These results support the assumption that the nature of the marker accepted by a recipient cell corresponds to the marker present at the replication point of the chromosome. In the absence of DNA and protein synthesis, the uptake of one marker seems to be successively followed by other markers in a linear order determined by the chromosome of the recipient cell.


Clustering of functionally related genes in Pseudomonas aeruginosa. Genes for the mandelate and benzoate pathways in Pseudomonas aeruginosa are clustered to a greater degree than that predicted on the basis of the induction pattern.


Phospholipid composition of Bacillus subtilis. Bacillus subtilis contained at least five phospholipids, four of which have been isolated and identified as a polyglycerol phospholipid, probably cardiolipin, phosphatidylglycerol, phosphatidylethanolamine, and lysylphosphatidylglycerol. Further purification of the latter phosphoglyceride was obtained by high-voltage electrophoresis, and it was shown that this treatment removed amino acid-containing, nonlipidic material from the phosphoglyceride. This associated material, which is not covalently linked to the lipid, gave rise to minor amounts of a number of amino acids, other than lysine, in acid hydrolysates of the lysylphosphatidylglycerol. The phospholipid composition of B. subtilis appeared to depend on the growth conditions. Addition of glucose to the medium lowered the pH during growth; this was accompanied by an increase in the amount of lysylphosphatidylglycerol and a decrease in the phosphatidylglycerol content, when compared with growth at neutral pH. The amount of the other phospholipids and the total amount of phospholipid remained constant under the different conditions. The shape and the osmotic susceptibility of the protoplasts of this organism appeared to depend on the growth conditions. Cells harvested from a neutral growth medium gave spherical protoplasts which lysed rapidly, whereas cells grown in an acidic medium maintained their rod-shaped form to a great extent after the cell wall had been removed, even after being suspended in a hypotonic medium. The latter observation suggests the presence of a more rigid membranous structure in cells which have been exposed to a low environmental pH during growth.


Estimation of thyroxine binding protein capacities using a non-electrophoretic technique.


Deoxyribonucleic acid base compositions of selected mycoplasmas and L-phase variants. Deoxyribonucleic acid base compositions were determined for 25 Mycoplasma strains and 6 L-phase variant strains. Values obtained correlated well with the results of other investigators.


William Murrell (1853-1912), clinical pharmacologist and toxicologist.


Characterization of the deoxyribonucleic acid of various strains of halophilic bacteria. Bacteria classified as extreme halophiles, in the genera Halobacterium and Halococcus, contain deoxyribonucleic acid (DNA) which displays two components in a CsCl equilibrium density gradient. The base composition of the major DNA component ranges from 66 to 68% guanine plus cytosine (GC), whereas that of the satellite DNA comprising some 11 to 36% of the total, is between 57 and 60% GC. Purification of the bacterial cells in a CsCl density gradient and other more conventional strain purification procedures both indicated that the presence of the satellite DNA component is not a result of mixed cultures.


Sudden sequential deafness as the presenting symptom of macroglobulinemia.


Factors affecting immunogenic activity of mycobacterial ribosomal and ribonucleic acid preparations. By following careful procedures, mycobacterial ribosomal fractions and ribonucleic acid (RNA) prepared by ethyl alcohol precipitation were obtained which have immunogenic activities similar to the viable attenuated H37Ra cells of Mycobacterium tuberculosis from which they were obtained. This comparison was based on the amount of ribonucleic acid (RNA) present. These preparations consisted of approximately 63% RNA and 37% protein; no deoxyribonucleic acid or polysaccharide was detected by chemical tests. A high correlation was found between the immunogenic activity of a preparation and the per cent increase in hyperchromicity at 260 nm of a ribonuclease-hydrolyzed portion. Final concentrations of sodium dodecyl sulfate higher than 0.25% when used for the preparation of the ribosomal fractions and RNA resulted in significantly lower immune responses and greater variation between experiments. This was not related to the amount of protein present. The stability of the ribosomal and RNA preparations was tested under a variety of conditions. The need for a good protective adjuvant again was shown since mouse serum readily hydrolyzed the RNA. Equal immunity was obtained after immunization by the intraperitoneal and subcutaneous routes; however, no immune response was obtained when the intravenous route was used. Preliminary results with RNA prepared with phenol showed that it was more easily degraded during preparation. This resulted in a lower immune response than was obtained with the RNA prepared with ethyl alcohol.


Influence of heparin on serum free thyroxine.


Carisoprodol toxicity.

Carisoprodol toxicity.


Fidelity of initiation of protein synthesis after premature chain termination in polarity mutants. beta-Isopropylmalate dehydrogenase, the product of the second cistron of the leucine operon in Salmonella typhimurium, produced by strains bearing nonsense or frameshift mutations in the first cistron of the operon was shown to be homogeneous as judged by electrophoretic and immunological techniques. Amino terminal analyses suggest that the enzyme produced by the mutant strains is identical with the wild-type enzyme. This view is supported by the observation that a nonsense mutant strain beta-isopropylmalate dehydrogenase copurifies with the wild-type enzyme. The results suggest that the uncoupling of normal chain termination and reinitiation does not interfere with the fidelity of subsequent polypeptide chain initiation in a polycistronic messenger ribonucleic acid.


Miocene monkey (prohylobates) from northern Egypt.


Modification of transcription following interaction of template specific monomers of Bacillus subtilis RNA polymerase.


Transition state analogues for enzyme catalysis.


Growth of microorganisms in the presence of transition metal complexes: the antibacterial activity of trans- dihalogenotetrapyridinerhodium(3) salts.


Presumed super-foetation in an Erythrocebus patas monkey.


Why is there a fovea in the human retina?


The incidence of neutralizing antibodies to duck plague virus in serums from domestic ducks and wild waterfowl in the United States of America.


Standard procedures for the isolation and identification of paratuberculosis organisms.


Antemortem diagnosis of bovine cysticercosis due to Taenia saginata.


Recovery of Brucella abortus, strain 19 from immunized cattle.


The effect of temperatures 60--100 degrees on human serum protein fractions.


Primate frontal lesions and the temporal structure of behavior.


Rapidly-labelled ribonucleic acid-protein complexes of the thyroid tissue.


Growth and development of infant M. mulatta fed a standardized diet.


Growth and development of the fetal rhesus monkey. I. Physical growth.


Synthesis of an analogue of mycobactin.

Synthesis of an analogue of mycobactin.


The cortical connections of the nucleus pulvinaris of the dorsal thalamus in the rhesus monkey.


The effect of urea on aggregation and incubation resistance of human platelets.


Platelet surface charge and aggregation. Effects of polyelectrolytes.


Protection against experimental Newcastle disease in chickens given Brucella bacteriophage.


Immediate visual memory as a function of epileptic seizure type.


Qualitative and quantitative aspects of thyroxine metabolism in sheep.


Immunochemical identification of thyroglobulin fragments synthesized in vitro by bovine thyroid polyribosomes.


Acquisition of thymidylate synthetase activity by a thymine-requiring mutant of Bacillus subtilis following infection by the temperate phage phi 3.


Electrophoretic characterization of bacterial polyribosomes in agarose-acrylamide composite gels.


The attenuation, with loss of oncogenicity, of the herpes-type virus of Marek's disease (strain HPRS-16) on passage in cell culture.


Evolutionary origin of the Gm"a" peptide of immunoglobulins.


Acrylic resin tooth implants in rhesus monkeys: a preliminary report.


The dental polymer implant concept.

The dental polymer implant concept.


Histamine and reflex vasodilatation in the rat.


Structural relationship and complement fixing activity of sheep and other ruminant immunoglobulin G subclasses.


Double-alternation learning by retardates.


Retention of delayed-alternation: effect of selective lesions of sulcus principalis. Monkeys with bilateral lesions of the anterior, miiddle, or posterior thirds of the principal sulcus, of the periarcuate prefrontal region, or of the inferior parietal lobule were tested for retention of spatial delayed-alternation. Lesions limited to the middle third of sulcus principalis resulted in failure to relearn delayedalternation within 1000 trials; lesions elsewhere had little effect.


Growth of the Singapore strain of Toxoplasma gondii in primary monkey kidney tissue cultures.


Studies of anticonvulsant levels in epileptics. I. Serum diphenylhydantoin concentrations in a group of medically indigent outpatients.


Neuropathological studies in three Scandinavian cases of progressive myoclonus epilepsy.


Tomography of the temporal bone in Paget's disee.


Epiphyseal dysostosis.

Epiphyseal dysostosis.


Congenital arteriovenous malformation of the foot.


Atherosclerosis in some species of New World monkeys.


Experimental atherosclerosis in primates: a comparison of selected species.


Atherosclerosis in nonhuman primates: sequences and possible mechanisms of change in phospholipid composition and metabolism.


Radio telemetry techniques for study of cardiovascular dynamics in ambulatory primates.


Heart transplantation in primates.

Heart transplantation in primates.


On the evolution of the craniocerebral topography in primates.


Discussion paper: antigenic factors characteristic of human immunoglobulin G, detected in the sera of nonhuman primates.


Discussion paper: investigations of nonhuman primate hemoglobin--electrophoretic variation.


Discussion paper: isoprecipitins to alpha 2-macroglobulins in chimpanzees.


Discussion paper: the expression of human genetic factors (Gm) on primate immunoglobulins.


Discussion paper: electrophoretic analysis of proteins of human and nonhuman primate stroma.


Discussion paper: studies of blood-oxygen affinity in adult and fetal macaque monkeys.


The effect of anemia upon cardiovascular performance and acid-base state of the fetal rhesus monkey.


Experiences in laboratory breeding of baboons.


The use of experimental surgical techniques in the pregnant Macaca mulatta.


Teratogeny in nonhuman primates, with notes on breeding procedures in Macaca mulatta.


Experiences in large-scale breeding of simians for medical experimentation.


Maintenance and handling of primate animals for medical research.


Marmosets: their potential in experimental medicine.


Need for specific pathogen-free monkeys in certain radiobiological studies.


Blood groups of apes and monkeys--their practical implications for experimental medicine.


Discussion paper: demonstrating of experimental techniques using nonhuman primates.


Discussion paper: use of primate animals in biological and medical research in Germany.


Experimental surgery in primates and in standard laboratory animals: a comparative survey.


Lung transplantation in the baboon.

Lung transplantation in the baboon.


Renal heterotransplantation from nonhuman primates to man.


The Rhesus monkey as a renal transplant model.


Characteristics of leukocyte and platelet antigens shared by nonhuman primates and man.


Surgical treatment of schistosomiasis by extracorporeal hemofiltration in baboons and in man: S. mansoni and S. haematobium.


Immunology of experimental transplantation in prite animals.


Current trends in use of marmosets in virological research.


Nonhuman primates in viral research.

Nonhuman primates in viral research.


Use of subhuman primates in experimental infections.


Discussion paper: onhuman primates in viral research.


Discussion paper: the use of primates in experimental viral infections--rubella and the rubella syndrome.


Value of nonhuman primates in predicting disposition of drugs in man.


Experimental epilepsy in primates.

Experimental epilepsy in primates.


Nonhuman primates in the study of toxicological effects on the central nervous system: a review.


Discussion paper: recent experimental data on the effects of Ritalin on chimpanzee psychophysical responding.


Nonhuman primates in evaluation of hematotoxicity.


The comparative approach to the role of nonhuman primates in evaluation of drug toxicity in man: a review.


Experimental atherosclerosis in primates and other species.


Experimental atherosclerosis in baboons.


Method for increasing atmospheric carbon dioxide in bacteriological incubators. We have adapted three incubators for culturing mycobacteria in an atmosphere of approximately 5% CO(2) and two incubators for culturing other organisms in atmospheres of approximately 10% CO(2).


Methyl anthranilate, an inhibitor for thegermination of spores of aerobic bacilli. Methylanthranilate inhibited the germination of spores of aerobic bacilli without affecting growth and sporulation. The inhibition of germination could not be reversed by removal of methylanthranilate.


In vitro susceptibility of Neisseria gonorrhoeae to nine antimicrobial agents. The in vitro action of nine antibiotics was tested by the agar streak method against 45 gonococcal strains isolated from penicillin-therapy failures. The penicillin susceptibility range of these strains was 0.003 to 1.32 mug/ml, and the tetracycline susceptibility range was 0.125 to 2.0 mug/ml. Minimal inhibitory concentrations of minocycline and doxycycline paralleled the activity of tetracycline and ranged from 0.125 to 1.0 mug/ml and 0.125 to 2.0 mug/ml, respectively. Rifampicin, with a narrow range of 0.5 to 1.0 mug/ml, inhibited 75% of the strains at 0.5 mug/ml. The range for cephaloridine and cephaloglycine was 0.5 to 20.0 mug/ml, but another cephalosporium derivative, cephalexin, exhibited greater activity in its range of 0.25 to 20.0 mug/ml. A semisynthetic penicillin, carbenicillin, with a range of 0.025 to 0.75 mug/ml, displayed more activity against the lower susceptible penicillin G gonococcal strains.


Thin-layer chromatography on sephadex for the identification of antibiotics.


Cross-reactions among group A streptococci. II. Further analysis of antigens related to type-specificity and protection.


Immunochemical studies of the phytohemagglutinin, concanavalin A.


The production of tolerance to human erythrocytes in the rat with cytosine arabinoside or cyclophosphamide.


Studies on the mechanisms of resistance to phylogenetically diverse intracellular organisms.


Comparative immune response to amphibian cytoplasmic viruses assayed by the complement-fixation and gel immunodiffusion tests.


Spontaneous degradation of pathologic macroglobulins in urea. A kinetic study.


Interaction of concanavalin A with polysaccharides of Histoplasma capsulatum.


High efficiency of SAC1gp4gp2gp conversion to S with EDTA-treated rat serum.


Antibody formation against 2,4-dinitrophenyl-hapten at the cellular level. Hemolytic plaque-in-gel assay for lymphoid cells producing anti-2,4-dinitrophenyl-hapten antibody.


Immunochemical studies on the poly-gamma-D-glutamyl capsule of Bacillus anthracis. V. The in vivo fate and distribution in rabbits of the polypeptide in immunogenic and nonimmunogenic forms.


Red venous blood: occurrence and significance in ischemic and nonischemic cerebral cortex.


The effects of ventrolateral thalamic lesions on tremor and the biosynthesis of dopamine in monkeys with lesions in the ventromedial tegmentum.


The postural effect of lesions of the vestibular nuclei: a note on species differences among primates.


Complications of intravenous hyperalimentation in newborns and infants.


Implant surgery for arterial injury and disease.


Cellular degeneration in the production of some mutant phenotypes in Drosophila melanogaster.


Comparison of immulogic properties of a high and low cell culture passage of transmissible gastroenteritis (tge) virus from a single isolant.


Thymidine triphosphate nucleotidohydrolase: a phage-induced enzyme in Bacillus subtilis.


Synthesis of DNA in phage-infected Bacillus subtilis.


Inhibition of bacterial DNA and protein synthesis in Bacillus subtilis by phage SP82. Effect of changes of temperature on the inhibition.


Conversion of the electrophoretic forms of cowpea mosaic virus in vivo and in vitro.


Intensive care unit of an accident hospital. Principles of treatment and analysis of the first 137 consecutive cases.


Influence of chlorophenoxyisobutyrate (CPIB) on thyroid parameters.


Relationships between blood thyrotrophin level, protein bound iodine and free thyroxine concentration in man under normal physiological conditions.


Neurological study of simulated decompression in supersonic transport aircraft.


The Fourth Harry G. Armstrong Lecture. Radiobiological factors in space conquest.


Avoidance and escape behavior controlled by artificial gravity.


Pathology of normobaric oxygen toxicity in primates.


Autoimmune antibodies to human lung in bronchial asthma.


Chloramphenicol and neomycin in combination.


Bone marrow reactions to trauma. Stimulation of erythropoietic marrow by mechanical disruption, fracture or endosteal curettage.


Supplying cell cultures regularly to distant laboratories.


Synthesis and function of messenger RNA during early embryonic development.


Mannosidosis: isolation of oligosaccharide storage material from brain.


Immunological studies on piroplasmosis of cattle. 3. Isolation of soluble antigen of Babesia bigemina and its serological properties.


Mannosidosis: a clinical and histopathologic study.


A five-year survey of skiing fractures from Falls Creek, Victoria.


The M. serratus posterior superior in certain catarrhine monkeys and man, in particular the structure of the muscular digitations and their nerve supply.


The influence of triiodothyronine (T3) treatment on the resin uptake of 131I-triiodothyronine.


Rubella vaccine: Gil P-48 strain in volunteers.


Transmissible mink encephalopathy: neuroglial response.


Chemical investigations of the molecular origin of biological radiation damage.


Phosphorus isocyanates.

Phosphorus isocyanates.


Nucleophilic substitutions with pyrimidine nucleoside N3 sodium salts.


The absolute configuration of bacteriochloro-phyll-alpha.


The relationship between selected blast-wave parameters and the response of mammals exposed to air blast.


Initiation and control of DNA synthesis.


Cricopharyngeal myotomy for pharyngo-esophageal diverticulum.


Impairments in selective attention to visual stimuli in monkeys with inferotemporal and lateral striate lesions.


The natural history and management of radiation induced injury of the gastrointestinal tract.


Haloperidol and electric shock interaction effects on tremors and on catecholamine concentrations in the caudate nucleus of the squirrel monkey.


Chromosomes in haematology.

Chromosomes in haematology.


The decline and fall of anti-Rh(D).

The decline and fall of anti-Rh(D).


Rhodesian tribal blood groups.

Rhodesian tribal blood groups.


Studies on peptides. XXII. Synthesis of the partially protected pentadecapeptide related to monkey and human beta-melanocyte-stimulating hormones.


Studies on peptides. 23. Total synthesis of monkey beta-melanocyte-stimulating hormone.


Some aspects of the evolution of metabolic pathways.


The functions of phosphoenolpyruvate carboxykinase and malic enzyme in the anaerobic formation of succinate by Ascaris lumbricoides.


Behavioral flexibility and intertroop variation among nilgiri langurs (Presbytis johnii) of south India.


Meiosis in five Macaca species.

Meiosis in five Macaca species.


Chromosomes and DNA synthesis in the stumptail monkey (Macaca speciosa), with special regard to marker and sex chromosomes.


The ontogeny of social behaviour of squirrel monkeys (Saimiri sciureus) in a seminatural environment.


Correlates of ecology and social organization among African cercopithecines.


Comparison of thyroxine-binding plasma proteins of various vertebrates and their evolutionary aspects.


A survey of immunohematologic proficiency among 131 clinical laboratories in Pennsylvania.


The healing of skin wounds in primates. 3. Behavior of the cells of Langerhans.


The distribution of cholinergic and monoaminergic neurons in the brain.


Hair digestion by a keratinase of Trichophyton mentagrophytes.


Experimental friction blisters in Macaque monkeys.


Impairment by d-amphetamine of delayed matching performance in monkeys.


Role of the lymphatics in the intestinal absorption and distribution of drugs.


Effects of lanthanum on contraction, calcium distribution and Ca45 movements in intestinal smooth muscle.


Brain stem infleunce upon limbic and prefrontal discharge activity.


Biochemical genetics of bacterial sporulation. I. Unidirectional pleiotropic interactions among genes controlling sporulation in Bacillus subtilis.


Fibrinogen turnover in pregnancy.

Fibrinogen turnover in pregnancy.


Hyperphagia and polydipsia in socially isolated rhesus monkeys. Three rhesus monkeys which had been isolated from social contact during their first year of life persistently overate and overdrank during adulthood. These monkeys ingested approximately twice as much fluid and food as the control animals reared normally.


Thermoregulation and norepinephrine.

Thermoregulation and norepinephrine.


Histuria during renal transplantation.

Histuria during renal transplantation.


Skin grafting in monkeys and apes.

Skin grafting in monkeys and apes.


Fibrinuria during renal transplantation.


The eradication of swine fever from Great Britain.


An observer reliability study of physicians' and nurses' decisions in utilization review of chronic-care facilities.


The Piromen test: a useful assay of bone marrow granulocyte reserves.


Intragastric buffering characteristics of a new concentrated antacid.


Dicloxacillin in beta-hemolytic streptococcal pharyngitis.


Comparative ultrastructure of selected aerobic spore-forming bacteria: a freeze-etching study.


A double-blind comparison of haloperidol with perphenazine in acute psychiatric episodes.


Haloperidol in the treatment of anxiety-tension states.


Psychopharmacologic characteristics of an indole compound--molindone.


Filamentous bacterial viruses.

Filamentous bacterial viruses.


Diffusion in the cow udder of sulfathiazole and two nitrofuran preparations in dimethyl sulfoxide (DMSO) after intramammary infusion.


The breast milk excretion on flufenamic acid.


Effect of citrate on acyl-coA synthetase of rat liver.


Effect of caffeine on adenylosuccinate lyase synthesis in Bacillus subtilis.


Receptor substance for pyocin R. I. Partial purification and chemical properties.


Changes of the copper states of porcine ceruloplasmin by urea denaturation.


Transaminase of branched chain amino acids. VI. Purification and properties of the hog brain enzyme.


Evidence for exclusive biosynthesis in vivo of serum albumin by bound polysomes of rat liver.


Fate of exogenous bacterial deoxyribonucleic acids in barley seedlings.


The uptake of tritiated uridine and phenylalanine by the ovaries of rats and monkeys.


Degree of superhelicity of covalently closed cyclic DNA's from Escherichia coli.


Critical comments on the quality of some commercial antivenins.


Studies of phospholipase A and B activities of Egyptian snake venoms and a scorpion toxin.


Allergens in pollen.

Allergens in pollen.


Amino acid sequences of tryptic peptides, alpha-T9, alpha-T10, and alpha-T11 from the alpha-polypeptide chain in Macaca mulatta monkey hemoglobin.


Amino acid sequence of the insoluble tryptic peptide, alpha-T12,13 from the alpha-polypeptide chain in Macaca mulatta monkey hemoglobin.


The amino acid sequence of th insoluble tryptic peptide, beta-T10, 11, 12 from the beta-polypeptide chain in Macaca mulatta monkey hemoglobin.


Considerations of some serologic reactions for autoantibodies against the liver.


Treatment of hepatolenticular degeneration. (Wilson's disease).


Effect of toxemia therapy on uterine dynamics.


Mannitol absorption and metabolism in man.


Aerial transmission of mycobacteria.

Aerial transmission of mycobacteria.


Geographic distribution of bacteriophage types of Mycobacterium tuberculosis.


Isoniazid and the survival of tubercle bacilli in airborne droplet nuclei.


Pulmonary mycetoma due to Allescheria boydii.


Effect of prophylactic isoniazid on early developing experimental tuberculosis in Macaca mulatta.


The microvasculature of the synovial membrane of the monkey: ultrastructural studies.


Enzymic and immunochemical properties of lysozyme. I. Derivatives modified at tyrosine. Influence of nature of modification on activity.


Interaction of kanchanomycin with nucleic acids. I. Physical properties of the complex.


PhospholipA2se A from Crotalus atrox venom. I. Purification and some properties.


Prolongation of canine kidney-homograft survival by heterologous ribonucleic acid (RNA) perfusion.


The solid-phase synthesis of polyglutamates of folic acid.


Profound selective cooling and ischaemia of primate brain without pump or oxygenator.


A test for separating secondary from primary bilateral synchrony in epileptic subjects.


Older patients in United States public mental hospitals: a national survey of administrative opinions.


Balagodu virus, a new arbovirus isolated from Ardeola grayii (Sykes) in Mysore State, South India.


Benign and malignant tumors and tumor-like conditions of fibrous tissue.


The management of the patient with advanced cancer.


Role of bivalent cations in the phosphoglucomutase system. I. Characterization of enzyme-metal complexes.


The effect of amino acid analogues on alkaline phosphatase formation in Escherichia coli K-12. 3. Substitution of 2-methylhistidine for histidine.


Unproven methods of cancer treatment. Rand coupled fortified antigen (RCFA) and delayed double diffusion (3-D) test.


Kinetics and specificity of the adenine nucleotide translocation in rat liver mitochondria.


Viruses and cancer.

Viruses and cancer.


The entry of P. papatasii into dwellings in Egypt.


Maintaining the oral and general health of the oral cancer patient. II.


Kinetics of phosphorylation of intramitochondrial and extramitochondrial adenine nucleotides as related to nucleotide translocation.


The effect of the amount of mycobacterial adjuvants on the immune response of strain 2, strain 13 and Hartley strain guinea pigs to DNP-PLL and DNP-GL.


The Third National Cancer Survey.

The Third National Cancer Survey.


The effect of carbamylation, amidination and benzylation on the ability of rabbit gamma-G antibody to produce passive cutaneous anaphylaxis in guinea pig and rabbit skin.


The immunoglobulins of equine colostrum and parotid fluid.


Particulate and soluble antigens of Rickettsia tsutsugamushi in the complement fixation test.


The specificity of the early immune response. I. A comparison of the specificity of delayed hypersensitivity and humoral responses following immunization with human gammaG globulin.


Immunologic and immunochemical studies on the gar, Lepisosteus platyrhincus. I. Immune responses and characterization of antibody.


Rabbit autoantibodies to aorta.

Rabbit autoantibodies to aorta.


Differentiation of human IgM subclasses by the ability to interact with a factor resembling the first component of complement.


Insoluble immunoadsorbents containing anti-IgE: removal of reaginic activity and subsequent elution.


Placentation in the rhesus monkey (Macaca mulatta).


Vision and education.

Vision and education.


The role of calcium and of in vitro aging on the creep behavior of collagen tendons.


Pathological changes in the reorganisation of the bone after transplantation of whole homogenous joints.


Liver blood flow studies during and after various periods of total biliary obstruction in the dog.


Magnetic and threshold techniques for energy calibration of high-energy radiations.


Natural infection of red Howler-monkeys, Alouatta seniculus straminea, with Plasmodium brasilianum, in the State of Amazonas, Brazil.


Intercomparison and calibration of high-energy x-rays and electrons.


Proposed National Bureau of Standards program for the calibration of instruments used in high-energy electron and x-ray beams.


Policy of the National Research Council of Canada on high-energy calibration.


The HPA and AAPM survey of depth-dose data for tele-isotope gamma rays and megavoltage x-rays.


AAPM-HPA survey of high-energy electron depth-dose data.


Calculated and measured dose distributions in inhomogeneous materials and in patients.


High-energy electron dose perturbations in regions of tissue heterogeneity.


The effect of inhomogeneities on dose distributions of high-energy electrons.


Treatment plans in homogeneous tissue, using electrons of 15 to 37 MEV.


Yersinia enterocolitica infection associated with brucella agglutinins. Clinical features of 24 patients.


Film dosimetry of high-energy electrons.


Chemical dosimetry for the measurement of high-energy photons and electrons.


Comparison of calorimetric and other methods for the determination of absorbed dose.


Thermoluminescent response of lithium fluoride to high-energy electrons.


Calculation of energy and charge deposition and of the electron flux in a water medium bombarded with 20-MEV electrons.


Discussion: a simple calorimeter for 6-MEV electrons.


The retinal pigment epithelium. Radiation thresholds associated with the Q-switched ruby laser.


Pilocarpine inactivation.

Pilocarpine inactivation.


Depigmentation of eyelids after topically administered thiotepa.


Incus replacement grafts in monkeys.

Incus replacement grafts in monkeys.


Release of diamine oxidase by heparin in the rat.


Metabolism in vitro and in vivo of pentazocine.


Studies with alkylating esters. I. The fate of ethylene dimethanesulphonate.


The differential effects of 8-azaguanine, 5-fluorouracil, 6-mercaptopurine and 6-thioguanine on the activities of some Krebs cycle enzymes in cell-free extracts from Bacillus cereus.


Connexions of the somatic sensory cortex of the rhesus monkey. I. Ipsilateral cortical connexions.


Effects in vivo and in vitro of nonsteroidal anti-inflammatory drugs on (rat stomach) histidine decarboxylase.


The pretectal syndrome in monkeys. I. Disturbances of gaze and body posture.


Brain damage in the rhesus monkey resulting from profound arterial hypotension. II. Changes in the spontaneous and evoked electrical activity of the neocortex.


Simian EEG activity related to problem solving during a simulated space flight.


The superior colliculus and higher visual functions in the monkey.


Brain damage in the rhesus monkey resulting from profound arterial hypotension. I. Its nature, distribution and general physiological correlates.


Coronary vasodilators on myocardial oxygen consumption and ammonia production.


Targets for tetracyclines.

Targets for tetracyclines.


Dimethylsulphoxide: a fallen idol.

Dimethylsulphoxide: a fallen idol.


Retinal vasculature in mammalia.

Retinal vasculature in mammalia.


Ectodermal dysplasias.

Ectodermal dysplasias.


Risk of methylmercury cumulation in man with refernce to consumption of fish contaminated with methylmercury.


Fluctuations in populations of 3 Vorticella species from an activated-sludge sewage plant.


The effect of colchicine on regenerating membranellar cilia in Stentor coeruleus.


What the peritrichs have taught us.

What the peritrichs have taught us.


Inconstancy of "constantly" infused penicillin.


Role of non-surface antigens in controlling Paramecium surface antigen synthesis.


Milk antibodies in familial dysautonomia.


Prevention, diagnosis, and treatment of lead poisoning in childhood.


Health advocates.

Health advocates.


Educating New York City residents to benefits of Medicaid.


Personal versus telephone interviews: effect on responses.


Lead tests by high-velocity impact of infectious specimen containers.


State laws on compulsory immunization in the United States.


Miniature pigs bred for research.

Miniature pigs bred for research.


Survey of state-level programs in mental health statistics.


Fractures around the ankle joint.

Fractures around the ankle joint.


Clinical presentation of the outbreak of the 1967 influenza epidemic.


Bacteriological safety of hot tapwater in developing countries.


Relationship of water fluoridation to bone density in two N.Y. towns.


Decline in mortality from carcinoma of the cervix in Beverly, Mass.


Functions of independent variables in research and program planning.


The use of hyperbaric oxygenation in radiotherapy.


Pyrazole inhibition and kinetic studies of ethanol and retinol oxidation catalyzed by rat liver alcohol dehydrogenase.


Nutrition by intraperitoneal fat infusion.


Epilepsy and 3 per second spike and wave rhythms. A clinical, electroencephalographic and prognostic analysis of 346 patients.


Histological observations on Wallerian degeneration in the spinal cord of the baboon, Papio papio.


The integration of donor and recipient deoxyribonucleic acid during transformation of Bacillus subtilis. 1. DNA labelled with 5-bromo[(3)H]uracil was used to transform auxotrophic strains of Bacillus subtilis. 2. After various times of incubation, DNA was extracted from the transformed culture and subjected to equilibrium sedimentation in caesium chloride gradients. 3. In addition to heavy donor DNA and light recipient DNA, a component with an intermediate density was found and is believed to consist of a biological hybrid of donor and recipient. 4. The component of intermediate density was isolated and found to possess activity in transformation derived from both donor and recipient strains. 5. Denaturation of the component of intermediate density followed by centrifugation gave only one component, indicating that integration had occurred in both strands of the recipient DNA. 6. No integrated band was observed after uptake by competent cells of B. subtilis of heavy DNA prepared from Escherichia coli.


The action of dilute aqueous NN-dimethylhydrazine on bacterial cell walls. 1. Walls of certain Gram-positive bacteria dissolved on incubation with dilute aqueous NN-dimethylhydrazine in the presence of air, by a reaction that probably involves free radicals. 2. Under the conditions described, the soluble products from the peptidoglycan were almost all non-diffusible. After brief incubation of walls of some organisms with reagent, part of the peptidoglycan component was obtained as a high-molecular-weight gel, the viscosity of which was rapidly decreased by incubation with lysozyme. 3. The extent to which peptidoglycan dissolved varied with different organisms, depending possibly on the extent of cross-linking, but the nature of the bonds that were destroyed has not been established. 4. Teichoic acids and polysaccharides were solubilized by this treatment and could be isolated in high overall yield. 5. The procedure is valuable in the examination of the distribution of heteropolymers in walls, and has been used to show that the polysaccharide present in walls of Lactobacillus arabinosus 17-5 is phosphorylated and may account for 20% of the total phosphate of the wall.


Susceptibility of patas monkeys to re-infection with infectious hepatitis.


In-vitro lysis of AET-treated normal red cells (PNH-like cells) by normal and cirrhotic sera.


Hypobaric erythraemia: pathology and coagulation studies.


Studies on immunization against Brugia malayi infection in the rhesus monkey. Recent studies on immunity to helminthic infection have shown that some degree of protective immunity may be stimulated by inoculations of attenuated living worms or their metabolites. The present experiments were designed to observe the effects of attempted immunization in the rhesus monkey by the use of attenuated infective larvae of Brugia malayi.Some effect was observed in animals inoculated with larval incubate, in which microfilaraemia did develop, but at low levels and for short durations. However, the most strking finding was that persistent immunity to challenge infections (expressed as failure to cause microfilaraemia) was obtained in animals vaccinated with large numbers (200) of infective larvae attenuated by X-irradiation at 20 000 R.Experiments with different numbers of larvae attenuated by different doses of irradiation suggest that there is an optimum combined effect of these two factors in eliciting functional antibody in a quantity sufficient to prevent patent infection in Malayan filariasis.


The dissociation of rabbit reticulocyte ribosomes with EDTA and the location of messenger ribonucleic acid.


Feeding and drinking following stimulation of the diencephalon of the monkey with amines and other substances.


Polymeric forms of free normal kappa and lambda chains of human immunoglobulin.


Late effects of 32 MeV proton irradiation in primates.


The inhibition of Listeria monocytogenes by an organism, resembling Bacillus mycoides, present in normal silage.


Studies on the mode of action of diphtheria toxin. Protein synthesis in guinea pig tissues and primary heart cell cultures.


Notes upon a trypanosome strain resembling T. congolense apparently completely adapted to the porcine species.


A convenient method for inserting small feeding tubes.


Dietary aspects of atheroslcerosis and thrombosis.


Kinetics and mechanism of hydrolysis of phenyl alpha-maltos- ide by saccharifying alpha-amylase of Bacillus subtilis. II. Dependence of the rates of formation of phenol, phenyl alpha-glucoside and maltotriose on the substrate concentration.


Microanalysis of action patterns of amylolytic enzymes: identification of a glucose-forming amylase in Japanese radish root.


Demonstration of an antibody to proximal tubular antigen in the pathogenesis of experimental autoimmune nephrosis in rats.


Serum antibody activity in patients with histoplasmosis as measured by passive hemagglutination.


The development of infant monkeys fed low phenylalanine diets.


A triple regimen of streptomycin, isoniazid and ethambutol in untreated cases of pulmonary tuberculosis.


Dark reticular cells in human lymphadenitis and lymphomas.


Experimental hemoglobinuric nephropathy. I. Comparative light microscopic, histochemical, and pathophysiologic studies.


Experimental hemoglobinuric nephropathy. II. Electron microscopic studies.


Nutritional value of lipids in Hydrogenomonas eutropha as measured in the rat. Hydrogenomonas eutropha is known to accumulate lipid, comprised largely of polymerized beta-hydroxybutyric acid, when maintained in nitrogen-deficient medium. This lipid was very poorly absorbed by mice from bacteria-containing diets, even though nitrogen absorption was adequate. The monomer, free beta-hydroxybutyric acid, was well absorbed from purified diet. Rats fed the monomer or butyric acid ate less food and grew more slowly than rats fed corn oil.


Efficiency of a multitest system (Enterotube) for rapid identification of Enterobacteriaceae. Enterotube, a multiple-test system which combines nine biochemical tests useful in the identification of members of the family Enterobacteriaceae, was tested and compared with the PathoTec test system in the identification of gram-negative bacilli isolated from clinical specimens. It was found that both the Enterotube and the PathoTec systems rapidly and accurately defined the position of the organisms in the major groups of the family Enterobacteriaceae. Discrepancies were noted between the results of citrate tests on Simmons' citrate-agar and in the Enterotube, as well as between Simmons' citrate-agar and the PathoTec citrate test. It was concluded that the Enterotube system provides a simple, reliable, and rapid method for the presumptive identification of Enterobacteriaceae. The major advantage of the Enterotube is that all tests are done simultaneously by inoculation from a single isolated colony.


Effects of systemic demethylchlortetracycline on human cutaneous microflora. The aerobic flora of the axilla and forehead of 20 normal human subjects was studied through a 3-week course of 600 mg of demethylchlortetracycline daily and a 3-week follow-up. In general in the axilla, an initial fall in bacterial density was followed by recovery in numbers due to the proliferation of resistant coagulase-negative cocci and diminution of the lipophilic diphtheroid group of bacteria within 1 week of treatment. During the remainder of the treatment period, the flora was essentially stable in both density and composition. In the follow-up phase, the initial flora was slowly re-established but resistant cocci persisted for more than 3 weeks. On the forehead the fall in density was slower and recovery during treatment was incomplete. Resistant coagulase-negative cocci became dominant and persisted through the follow-up phase. In two individuals, temporary colonization of the axilla by Staphylococcus aureus was seen.


Comparative sensitivity of tissue cultures to rubella virus: use of guinea pig cells for virus titration. A series of 19 different primary and serial tissue cultures were investigated for their sensitivity to virulent or attenuated rubella virus (RV). Primary guinea pig tissues, a serial passage of baby hamster kidney, and primary human amnion were comparable to African green monkey kidney tissue cultures in their sensitivity. In general, primary human tissues were relatively insusceptible to the Gilchrist strain of RV. RV interfered with the growth of vesicular stomatitis virus. Based on this finding, it was possible to develop an assay method in guinea pig tissue cultures by using VSV as the challenge virus. This system appeared to be comparable in sensitivity to the use of primary monkey kidney tissue cultures for the detection of small amounts of RV and offers the advantages of economy, rapidity, and safety.


Simple technique for dark-field photography of immunodiffusion bands. A simple dark-field photographic technique was developed which enables laboratory personnel with minimal photographic training to easily record antigen-antibody patterns on immunodiffusion plates.


Method for eliminating anticomplementary activity in animal sera. A method is described for removing the anticomplementary activity of sera by the addition of normal porcine serum. Results of sera taken from four animal species immunized with influenza, coxsackievirus, echovirus, and vaccinia viral antigens are presented.


Negative-pressure isolator for work with hazardous infectious agents in monkeys. The development, fabrication, and use of a negative-pressure isolator for work with hazardous infectious agents in monkeys or other larger animals are described.


Studies on the influence of immunodepressant drugs on blastic transformation and tissue respiration in immunologically active organs.


Biotransformation of glyceryltrinitrate in rabbits.


Dextran production by a human oral strain of Lactobacillus casei.


The hydroxy acids of Pseudomonas aeruginosa 8602.


Cleavage of bacterial flagellin with cyanogen bromide. Antigenic properties of the protein fragments. 1. Four polypeptide fragments, obtained by cyanogen bromide treatment of the protein flagellin from Salmonella adelaide, were tested for their antigenic activity by using them as inhibitors in three different assays: bacterial immobilization, haemagglutination of sensitized erythrocytes and quantitative micro precipitation. Immunodiffusion studies were also performed on the protein fragments. 2. Cleavage of the flagellin molecule in this way gave no detectable loss of antigenic determinants. Fragment A (mol.wt. 18000), the largest of the polypeptides, contained all the antigenic specificities present on flagellin that were recognized by the antisera used. In one test, fragment B (mol.wt. 12000) also contained antigenic activity to an extent not easily explainable by contamination with fragment A. Fragments C (mol.wt. 5500) and D (mol.wt. 4500) appeared to be antigenically inactive.


Studies on the antigenic value and premunition against bovine theileriosis due to Theileria annulata (Dschunkovsky and Luhs 1904) in Iran and Conservation of strains of Theileria annulata at-70degrees C.


The pathogenesis of Aleutian disease of mink. I. In vivo viral replication and the host antibody response to viral antigen. Mink inoculated with 1 x 10(5)ID(50) of Aleutian disease virus revealed very high virus titers in the tissues 8-18 days later. The highest virus titers observed were 5 x 10(8)ID(50) per g of spleen and 1 x 10(9)ID(50) per g of liver 10 days after inoculation. Concomitant with the increase in infectious virus titers, viral antigen(s) was found in the cytoplasm of macrophages in the spleen and lymph nodes and in Kupffer cells in the liver. Antiviral antibody was assayed by indirect immunofluorescence, using sections of infected liver as the source of antigen. A few mink infected for 9 days and all those infected 10 days or more developed antibody to Aleutian disease virus antigen(s). By 60 days after infection, when hypergammaglobulinemia was marked, the mink had an exceptionally high mean antibody titer of 100,000. The pathogenesis of the glomerulonephritis of Aleutian disease is apparently related to formation of viral antigen-antibody-complement complexes which lodge in glomerular capillaries. No evidence was found that viral infection of the kidney took place, and no autoimmune responses were found. In this "slow-virus" disease the virus replicates rapidly and the morphologic and biochemical manifestations of disease are apparently due to the continuing interplay between a replicating antigen and the host immune response.


Lactoferrin, an iron-binding protein in neutrophilic leukocytes. Lactoferrin, an iron-binding protein previously shown to occur in many external secretions, is identified as one of the major proteins present in human and guinea pig neutrophilic polymorphonuclear leukocytes. The identification of this protein in leukocyte extracts was based upon a comparison of its electrophoretic, antigenic, and iron-combining properties with the corresponding properties of the same protein isolated from human and guinea pig milk. Immunochemical quantitations showed that lactoferrin occurs in human neutrophilic leukocytes at the concentration of 3 microg per 10(6) cells. Tissue cultures from guinea pig bone marrow and spleen actively synthesized the protein, as shown both by net production of lactoferrin and incorporation of labeled amino acids into the protein. Immunohistochemical data indicate that lactoferrin first appears in myeloid cells at the stage of the promyelocyte.


Epidemiological surveillance programme in diphtheria during the stage of low morbidity.


Contribution to the study of the antigenic structure of some representatives of the aspergillus family using specific precipitation in agar.


Production of extracellular toxin by Listeria monocytogenes in vitro. Preliminary report.


Experimental allergic encephalomyelitis in the rhesus monkey. An electron microscopic study.


Effects of low irradiation doses on the Ultrastructure of the rat myocardium.


Increased autophagocytosis in renal proximal tubules during experimental "autoimmune" nephrosis.


Analysis of an apparent case of "gene-controlled mutational stability": the auxotrophic preemption of a specific growth requirement.


The use of oxytocin in obstetrics.

The use of oxytocin in obstetrics.


Suspected mutant of Agrobacterium tumefaciens with an altered DNA.


Development of drug resistance to rifampicin.


Anaesthesia for cerebral angiography: cardiovascular and cerebrospinal fluid pressure observations in the monkey under pentobarbital-halothane anaesthesia.


A clinical pharmacological evaluation of benzquinamide, a new antiemetic agent.


A controlled trial with a new preparation of etilefrini chloridum with prolonged effect (Effortil Prolongatum) on orthostatic reactions.


One-year evaluation of furosemide-reserpine combination in essential hypertension.


Effect of treatment with Carica papaya on resolution of edema and ecchymosis following rhinoplasty.


A double blind study of acetaminophen used in conjunction with oral anticoagulant therapy.


Parenteral haloperidol in controlling patient behavior during acute psychotic episodes.


A comparative trial of oxypertine and chlorpromazine in chronic schizophrenia.


Thioridazine in patients with depression.


The absorption and excretion of penicillin G and triple sulfas in four pediatric preparations in normal children.


Laboratory control of heparin therapy with the activated partial thromboplastin time test.


Bacterial sensitivity to antibiotics, 1964-1968.


The effectiveness of nitrazepam in a refractory epileptic population.


Gas sterilizer evaluation with sporicidal testing.


Thioridazine suspension in paediatric practice--a cooperative study.


Psychotropic-anticholinergic management of gastrointestinal disorders.


One-year trial of the antipsychotic acridan compound, SK&F 14336.


Cardiotoxic effects of a new butyrophenone compound. U-25,927.


A new butyrophenone derivative. AL-1021.


Clinical trial of imidazoline (DH-524) as an antidepressant. I. Browsing phase.


Evaluation of thiazesim (SQ 10,496) as an anti-depressant: a negative report.


Intravenous lincomycin in high doses.

Intravenous lincomycin in high doses.


Tuberculosis of the myocardium.

Tuberculosis of the myocardium.


Actual possiblities of prophylaxis of rhesus iso-immunization.


The current status of nitrites in the treatment of coronary artery disease.


The ecology of drug-resistant tubercle bacilli in Eastern Glasgow.


Experiences with a new tuberculosis diagnostic test (percutaneous mycobacterium test) in the diagnosis and detection of tuberculosis.


WHO Expert Committee on Specifications for Pharmaceutical Preparations. 22.


Supination-eversion injuries of the ankle joint (grade 2 in Lauge-Hansen's classification). A series treated conservatively.


Side-effect of topical Thio-tepa.

Side-effect of topical Thio-tepa.


Granulocytic pyrogen: partial purification and demonstration of anticoagulant action.


Renal vascular responses to isoproterenol.


Effect of octanoate on carbohydrate metabolism in red and white muscle of the rhesus monkey.


Histopathology of experimentally induced nutritional deficiency cheilosis in the marmoset (Callithrix jacchus).


In vitro production of alpha-amylase by monkey parotid gland.


Studies in urinary tract infections. II. Urinary tract infection due to coagulase-negative staphylococci.


Interhemispheric projections of the parietal lobe in the rhesus monkey.


Central integration of visual half-fields in split-brain monkeys.


Secondary glaucoma in infancy and childhood. A review.


Bacterial decontamination of polymethyl methacrylate in ophthalmology.


Conjugation in Pseudomonas aeruginosa.

Conjugation in Pseudomonas aeruginosa.


Host specificity of DNA and conjugation in Pseudomonas aeruginosa.


A comparative study of the adrenergic innervation of the iris and ciliary structures in 18 species in phylogenesis.


Brain tumour and pregnancy.

Brain tumour and pregnancy.


Hawaii Medical Association's concern with medical care costs.


Eye movements evoked by stimulation of frontal eye fields.


Visual receptive fields of striate cortex neurons in awake monkeys.


Efficacy of rifampin in treatment of meningococcal carriers.


Chemoprophylaxis of meningococcal carriers.


Separation of microbial deoxyribonucleic acids into complementary strands. DNA preparations from seven bacterial species and from the E. coli phage T4 can, after denaturation with alkali, be separated chromatographically into two distinct components (L and H) through intermittent gradient elution from methylated albumin kieselguhr columns. The direct chemical analysis of the L and H fractions isolated from DNA specimens of the AT type shows them to exhibit a high degree of complementarity; but despite a bias in the distribution of purines and pyrimidines, either fraction contains equimolar quantities of 6-amino and of 6-keto nucleotides. In the L and H components derived from DNA of the equimolar and GC types, the distribution bias appears limited to guanine and cytosine. It is suggested that the L and H fractions represent the complementary DNA strands.


Sensitivity test and side effects of ethambutol.


Chemistry and biology of wax D from an unclassified mycobacteria P6, scotochromogen.


Replication of influenza virus in a continuous cell line: high yield of infective virus from cells inoculated at high multiplicity.


WHO Expert Committee on Biological Standardization. Twenty-first report.


Sructural proteins of adenoviruses. 3. Purification and characterization of the adenovirus type 2 penton antigen.


What has happened to avian monocytosis?

What has happened to avian monocytosis?


Structure and functions of mesosomes of gram positive bacteria.


Kinetics of injury and repair in monkeys and dogs exposed to gamma-ray fractionation.


Toxemia and its anesthetic management.

Toxemia and its anesthetic management.


Resuscitation and intensive care of the newborn infant.


Biologic requirements and control of equine viral arteritis vaccine.


Enzymatic analysis of the requirement for sodium in aerobic growth of Salmonella typhimurium on citrate. Na(+) was required for the aerobic growth of Salmonella typhimurium on citrate, but not on l-malate, glucose, or glycerol. The maximal growth rate and the maximal total growth occurred with 6 to 7 mm Na(+). Na(+) could not be replaced by K(+), NH(4) (+), Li(+), Rb(+), or Cs(+). Sonically treated extracts of citrate-grown cells contained the enzymes of the citrate fermentation pathway (citritase and oxalacetate decarboxylase) and all of the enzymes of the citric acid cycle. Thus, two separate routes of citrate catabolism appeared to be operational in the cells. Two discrete oxalacetate (OAA) decarboxylases were also demonstrated. One was of the "classic" type, being activated by Mn(++) and inhibited by ethylenediaminetetracetate (EDTA). It was present in the cell sap. The second decarboxylase closely resembled the Na(+)-activated OAA decarboxylase of citrate-grown Aerobacter aerogenes, whose growth also requires, or is increased, by Na(+). This decarboxylase was EDTA-insensitive, specifically activated by Na(+) and inhibited by avidin, and it had a high affinity for OAA. It was induced by growth on citrate, but not l-malate or glycerol. It is suggested that the Na(+) requirement for growth reflects the need to activate this OAA decarboxylase as a component of the citrate fermentation pathway and that citrate catabolism via the citric acid cycle, which should be independent of Na(+), is somehow dependent upon the activity of the Na(+)-activated enzyme.


Isolation of an antigen of Neisseria gonorrhoeae involved in the human immune response to gonococcal infection. Ion-exchange chromatography was used to isolate a fraction from disrupted gonococci which reacts with sera from patients with gonococcal disease in complement-fixation and gel-diffusion tests. This antigenic fraction was shown to be the same as that previously described as having been isolated by gel filtration. The method reported here has the advantage of greater rapidity of isolation together with some improvement in purity.


Effect of bacteria on chemotaxis in the cellular slime molds. The effect of chemotactic substances, secreted by Escherichia coli, on the cellular slime molds was studied by deposition of bacteria near myxamoebae populations. Droplets of a bacterial suspension and a myxamoebae suspension were placed separately, at predetermined distances from each other, on a hydrophobic agar surface of low rigidity. Myxamoebae remained confined inside the droplets, except when they were activated by the bacterial products. The sphere of attraction increased at higher bacterial concentrations. Myxamoebae could be attracted over distances as great as 5 mm. Myxamoebae in droplets close to dense bacterial populations not only were attracted toward the bacteria but also moved out in an opposite direction from the bacteria. There was a gradual decrease of attraction at increasing distances between amoebae and bacteria. The attraction by bacteria or bacterial products was reduced at lower temperatures. Light did not affect the distance over which attraction could be observed. Myxamoebae close to their aggregation phase were most sensitive to the bacterial attractants. Bacterial attractants at high concentrations could disperse aggregates, even when they were in an advanced stage. At still higher concentrations of the bacterial products, cells stopped moving altogether. The bacterial attractants activated different species of cellular slime molds. They appeared to be present not only in E. coli but also in all other bacterial species that were tested. These results are discussed in the light of earlier observations on the attraction of cells by aggregates of myxamoebae.


Evidence for a multiplicity of capsular types among Staphylococcus aureus strains. The Smith diffuse variant and the wound mucoid strain of Staphylococcus aureus were shown to exhibit serologically distinct capsules. The Welwood and K-6 strains of S. aureus were tested to determine their capsular types. Both Welwood and K-6 were found to be representative of the Smith capsular type. An additional 13 isolates of S. aureus from mice were tested. Gel double-diffusion tests and immunoelectrophoresis of staphylococcal antigens disclosed the possible existence of at least two additional capsular types. Passive hemagglutination tests carried out with cells sensitized with 1 mg of antigen per ml showed a multiplicity of cross-reacting antigens. However, cells sensitized either with 0.1 or 0.05 mg of antigen per ml and reacted with antisera absorbed with 10 or 1 mug/ml showed the presence of a specific antigen in each strain of S. aureus. Corroborative evidence for a multiplicity of capsular types was obtained by the specific capsular reaction. At least four capsular types of S. aureus were found. The prototypic strains for these antigens are the RLM or wound strain, the Smith diffuse strain, and mouse strains designated 36T and 43R. We propose to designate these types 1, 2, 3, and 4, respectively.


Transcriptional mapping: functional organization of the ribosomal and transfer ribonucleic acid cistrons in the Bacillus subtilis genome. The transcriptional mapping method developed previously has been used to investigate the functional organization of the ribosomal ribonucleic acid (rRNA) and transfer ribonucleic acid (tRNA) cistrons in the Bacillus subtilis genome. The sensitivities of the syntheses of these RNA species to actinomycin D indicated that (i) large polycistronic groupings of the tRNA cistrons exist, but (ii) the 16S and 23S rRNA cistrons are probably transcribed as individual units, and (iii) each 5S rRNA cistron is part of an operon containing a 23S rRNA cistron.


Estimation and identification of Streptococcus dysgalactiae protective antibody in sera of rabbits and cattle. Rabbit and cow anti-Streptococcus dysgalactiae sera were tested by bacterial agglutination, complement fixation, hemagglutination, and immunodiffusion for the presence of antibody. The results of these tests were compared with mouse-protection studies on the same serum to estimate which in vitro test would best reflect the in vivo protective capacity of serum. Identification of the antibody constituents responsible for the mouse protection, hemagglutination, and complement fixation titers were established by reacting whole and diluted antisera with mercaptoethanol before and after testing. Results indicate that the complement fixation test may be a more accurate indicator of IgG protective bovine and rabbit antibody, whereas the hemagglutination test may more readily reflect a wider range of protective antibody levels and IgM. The complement fixation test showed some shared responses to IgG and IgM in both the rabbit and cow, whereas the IgM components seemed to be the predominant factor influencing hemagglutination titers in the rabbit and more so in the bovine. Mouse protection tests with mercaptoethanol-treated cow and rabbit sera indicate that the protective capacity of these antisera is shared between IgM and IgG components.


Ultrastructure of Brucella abortus L-forms induced by penicillin in a liquid and in a semisolid medium. Brucella abortus L-forms were induced by 5.0 or 10.0 mug of penicillin/ml in a broth medium containing 0.3 m sucrose, and in a semisolid medium containing 10% calf serum and 20.0, 40.0, or 60.0 mug of penicillin/ml. After 96 hr of incubation, L-forms of various sizes and shapes were observed. Basic structures of the L-forms were similar whether induced in liquid or semisolid medium. L-forms had two "unit" membranes, each consisting of two outer dense layers separated by a lucent layer. A few large, irregularly shaped organisms in penicillin-treated broth cultures had additional surface material and were referred to as "transitional" forms. In contrast with L-forms, the bacterial cells were fairly uniform in size and shape, were smaller, and had a more complex cell wall structure. Small bodies limited by a "unit" membrane were present within and around numerous L-forms from liquid and semisolid medium cultures. Other internal membranous structures were also seen in some L-forms. Most Brucella L-forms described in this paper reverted to bacteria in the absence of penicillin and were structurally characteristic of unstable L-forms.


Gelatin-induced reversion of protoplasts of Bacillus subtilis to the bacillary form: biosynthesis of macromolecules and wall during successive steps. Protoplasts of Bacillus subtilis plated on SDG medium formed L colonies in quantative yield and propagated in the L-form indefinitely. Protoplasts or L bodies placed in 25% gelatin medium formed bacillary colonies. Details of the reversion of these naked bodies to the walled form are reported here. Protoplasts prepared in minimal medium reverted fairly synchronously 3 to 4 hr after inoculation into gelatin, but protoplasts preincubated in casein hydrolysate (CH)-enriched minimal medium were primed to revert within 1 hr in the gelatin. Preincubation for 1.5 hr in 0.44% CH was required for good priming. Cells must be subjected to this preincubation (step 1) in the naked state; it is effective for L bodies as well as protoplasts. Priming was blocked by chloramphenicol, puromycin, and actinomycin D but was not affected by penicillin, lysozyme, or inhibition of deoxyribonucleic acid (DNA) synthesis. It is concluded that protein and ribonucleic acid (RNA) synthesis are required during step 1, that DNA synthesis is not required, and that wall mucopeptide is not made. The reversion of well-primed protoplasts in the gelatin (step 2) proceeded undisturbed in thymine-starved cells with chromosomes arrested at the terminus. It was scarcely slowed by chloramphenicol in the gelatin but was delayed about 3 hr by both puromycin and actinomycin D. Escape from inhibition occurred while the inhibitors were still actively blocking growth. Penicillin and cycloserine inhibited and lysozyme reversed reversion. Momentary melting of the gelatin delayed reversion. It is concluded that mucopeptide synthesis occurs in step 2, that concomitant RNA, DNA, or protein synthesis is not essential, but that physical immobilization of excreted cell products at the protoplast surface is necessary early in step 2. Newly reverted cells were misshapen and osmotically sensitive. Processes which confer osmotic stability after reversion (step 3) did not occur in the presence of chloramphenicol or actinomycin D.


Intracellular inclusions resulting from infection with African horsesickness virus. Intranuclear and intracytoplasmic inclusions in monkey kidney cells infected with African horsesickness virus were studied with the aid of electron microscopy.


Evidence that Bacillus subtilis bacteriophage SP02 is temperate and heteroimmune to bacteriophage phi-105. Some characteristics of Bacillus subtilis phage SPO2 which show that it is a temperate phage are presented. Wild-type SPO2 forms turbid plaques, similar to those of other temperate phages. SPO2 lysogenic strains which are resistant to SPO2 can be isolated; these strains remain stable lysogens despite the fact that they can no longer adsorb SPO2. SPO2 lysogenic strains can be grown for many generations in SPO2 antiserum and remain lysogenic. Phage SPO2 plates on phi105 lysogens and phage phi105 plates on SPO2 lysogens; this indicates that SPO2 and phi105 are heteroimmune. Phage phi105 plates on an SPO2-resistant strain; this indicates that SPO2 and phi105 adsorb to different receptor sites on the bacterial surface.


Mechanism of transfection with deoxyribonucleic acid from the temperate Bacillus bacteriophage phi-105. Bacteriophage phi105 is a temperate phage for the transformable Bacillus subtilis 168. The infectivity of deoxyribonucleic acid (DNA) extracted from mature phi105 phage particles, from bacteria lysogenic for phi105 (prophage DNA), and from induced lysogenic bacteria (vegetative DNA) was examined in the B. subtilis transformation system. About one infectious center was formed per 10(8) mature DNA molecules added to competent cells, but single markers could be rescued from mature DNA by a superinfecting phage at a 10(3)- to 10(4)-fold higher frequency. Single markers in mature DNA were inactivated at an exponential rate after uptake by a competent cell. Prophage and vegetative DNA gave about one infectious center per 10(3) molecules added to competent cells. Infectious prophage DNA entered competent cells as a single molecule; it gave a majority of lytic responses. Single markers in sheared prophage DNA were inactivated at the same rate as markers in mature DNA. Prophage DNA was dependent on the bacterial rec-1 function for its infectivity, whereas vegetative DNA was not. The mechanism of transfection of B. subtilis with viral DNA is discussed, and a model for transfection with phi105 DNA is proposed.


Isolation of measles virus from cell cultures of brain from a patient with subacute sclerosing panencephalitis.


Long-term total parenteral nutrition in infants.


Immunochemical reactions of lipids.

Immunochemical reactions of lipids.


The genetics of thyroid disease.

The genetics of thyroid disease.


Coping with the summer heat.

Coping with the summer heat.


High-energy heavy charged particles in medicine.


Radiation therapy with electron beam: current techniques.


Fast neutrons for radiation therapy.

Fast neutrons for radiation therapy.


The interaction of pesticides with other factors.


Chloroquine ocular toxicity.

Chloroquine ocular toxicity.


Should we transplant organs or efforts?

Should we transplant organs or efforts?


The etiology of uric acid urolithiasis following ileostomy.


Transthoracic, transgastric interruption of bleeding esophageal varices.


Erythropoietin release after aortic coarctation in the baboon.


Silver and intestinal flora: Roles in bacterial colonization of burn wounds.


Vaccinia virus directed RNA and protein synthesis in the presence of rifampicin.


Role of 5S ribosomal RNA in polypeptide synthesis.


Changes in the cell-wqall composition of a strain of Bacillus subtilis grown in a chemostat.


Subtypes of rabbit kappa light polypeptide chains associated with the beta locus.


Structural and functional roles of the cysteine residues in the alpha subunit of the Escherichia coli tryptophan synthetase. I. Structural roles and reactivity of the cysteine residues.


Chemical modification of amino groups in staphylococcal enterotoxin B.


The role of iron in the reaction between rabbit transferrin and reticulocytes.


The utilization and metabolism of intravenous sorbitol.


Foetal bilirubin metabolism.

Foetal bilirubin metabolism.


Tolerance to prolonged hypovolaemic shock: the effect of infusion of an energy substrate.


Independent genetic control of the catalytic activity and the rate of degradation of catalase in mice.


Induction of histidine-degrading enzymes in protein-starved rats and regulation of histidine metabolism.


A useful thin-layer chromatography method for the determination of cytidine aminohydrolase activity in serum.


Parameters of thyroid function in maternal and cord serum at term pregnancy.


Application of metabolic data to the evaluation of drugs. A report prepared by the committee on problems of drug safety of the drug research board, National Academy of Sciences-National Research Council.


Influence of repetitive dosing of tetracyclines on biologic half-life in serum.


A study of antibody response in leprosy.


Kinetics of the reversible inhibition of enzyme-catalysed reactions by tight-binding inhibitors.


The two-haem nitrite reductase of Micrococcus denitrificans.


Human platelet phosphorylase.

Human platelet phosphorylase.


Reversible disaggregation of Escherichia coli succinyl-CoA synthetase.


The accessory optic system in Erinaceus (Insectivora) and Galago (primates).


Form perception and pattern reproduction by monkeys.


Limbic lesions and error reduction.

Limbic lesions and error reduction.


The release of catecholamines by shocks and stimuli paired with shocks. One monkey and five baboons were surgically prepared so that heart rate and blood pressure could be monitored continuously, and an extra-corporeal blood path was established to detect the secretion of epinephrine (adrenaline) and norepinephrine (noradrenaline). A respondent conditioning procedure was used in which a tone was paired with electric shocks. Epinephrine, but not norepinephrine was released by shocks, and a corresponding release was demonstrated by the tone alone. Heart rate and blood pressure changes were also elicited by shocks and by the tone.


Isolation and properties of lipoproteins from normal rat serum. Three major classes of lipoproteins (VLDL, d <1.006; LDL, d 1.006-1.040; HDL, d 1.063-1.21) were isolated by ultracentrifugal flotation from the serum of normal male Sprague-Dawley rats. Their physical, chemical, and immunological properties were analyzed and compared with those of their water-soluble, essentially lipid-free derivatives. Studies were also carried out on the d > 1.21 fractions. Each product was found to have distinct characteristics, and this was also indicated by spectral analyses carried out by the techniques of circular dichroism and UV absorption spectroscopy. The results provided evidence for the mutual role of the protein and lipids in determining the structure, and perhaps the immunological specificity, of serum lipoproteins.


Relation of mitochondrial phospholipase A activity to mitochondrial swelling. Assays of mitochondrial phospholipase A activity and mitochondrial swelling demonstrated that the phospholipase A activity is related to the swelling under the experimental conditions used. Both were stimulated by added free fatty acid and CaCl(2), not affected greatly by the addition of monoacyl phosphoglycerides, and inhibited by EDTA. The amount of fatty acid hydrolyzed from endogenous phosphatidyl ethanolamine and phosphatidyl choline during swelling was calculated to be 20-30 times less than the amount of added free fatty acid that gave comparable swelling. Under the experimental conditions about 4% of the phospholipid was hydrolyzed. Mitochondrial swelling was studied by electron microscopy and turbidity measurements. The results found were in agreement, whether oleic acid was present or not, except for those values obtained after very brief incubation (1 min) and after incubation for longer than 35 min. The lack of direct proportion between swelling and the concentration of lysosomes present indicated that the swelling is related mainly to mitochondrial phospholipase A, although swelling due to contaminating lysosomes cannot be excluded entirely. The temperature dependence of spontaneous, fatty acid-induced, or CaCl(2)-induced swelling suggested that enzymatic activities are responsible for swelling.


Fluorescence detection: application to the study of cerebral blood flow.


Effects of age, experience, and malnourishment on object retention in learning set.


Hippocampal theta activity and learning: a reply to Douglas.


Health services for the Poor People's Campaign.


Biochemical and bioelectric processes related to sleep, paradoxical sleep, and arousal.


Survey of suicide counseling available to students in metropolitan Richmond.


Immediate effects of smoking on healthy young men.


National Conference on Mental Health in Public Health Training.


Family planning policies and activities of state health and welfare departments.


Rubella antibodies in Rhode Island women of childbearing age.


The Federal budget process.

The Federal budget process.


Detection of phenylketonuria carriers.

Detection of phenylketonuria carriers.


Penetration of turtle eggs by Salmonella braenderup.


A few characteristics of patients in urban tuberculosis clinics.


Use of a single dose of doxycycline monohydrate for treating gonorrheal urethritis in men.


Psychiatric disorders among the patients of general practitioners and internists.


Measurement and application of illness costs.


Planning for the distribution of personal health services.


Automated multiphasic health testing.

Automated multiphasic health testing.


Health study of adolescents enrolled in the Neighborhood Youth Corps.


A survey of group practice in the United States, 1965.


Planning, model cities style.

Planning, model cities style.


An evaluation of immunization status of white children in a Kentucky County.


A skin test survey of tularemia in a Montana sheep-raising county.


Training the disadvantaged as home health aides.


Uses of a recordkeeping system to evaluate rat infestation and to develop control programs.


Revaccination against smallpox.

Revaccination against smallpox.


Variables related to a referendum vote on creating a county health department.


Relationship between comprehensive and environmental health planning.


Health concerns and attitudes regarding fluoridation.


Average age at death of scientists in various specialties.


Statistical aspects of the proportion method for determining the drug-resistance of tubercle bacilli.


A catecholamine storage in the pancreatic A2-cells of owl monkey (Aotes trivirgatus).


Phosphoenolpyruvate carboxytransphosphorylase. IV. Requirement for metal cations.


Nucleic acid reassociation in formamide.


Mode of action of berninamycin. An inhibitor of protein biosynthesis.


Fractionation of human serum high density lipoprotein in urea solutions. Evidence for polypeptide heterogeneity.


Immunochemistry of sperm whale myoglobin. IV. The role of the arginine residues in the conformation and differentiation of their roles in the antigenic reactivity.


Potentiation of inhibitory activity of colistin on Pseudomonas aeruginosa by sulphamethoxazole and sulphamethizole. Potentiation of colistin by sulphamethoxazole and sulphamethizole was demonstrated with 19 out of 20 strains of Pseudomonas aeruginosa. This enhancement was bactericidal as well as bacteriostatic. Synergy between trimethoprim and sulphamethoxazole was also demonstrated with four strains of Ps. aeruginosa, but even when the two drugs were combined high concentrations of trimethoprim were still required to produce a bactericidal effect. Combinations of sulphamethoxazole and gentamicin appeared to be synergistic when the bacteriostatic effect was measured, but the combined bactericidal effect was indifference. The bactericidal and bacteriostatic effect of combinations of carbenicillin with sulphamethoxazole was also indifference.


Waldenström's macroglobulinaemia. An unusual case having only pleuropulmonary manifestations.


Uptake of Zn65 and Mn54 by irradiated Bacillus megaterium.


Cortical responses to light in unanesthetized monkeys and their alteration by visual system lesions.


Lens proteins and the systematic position of the Musophagiformes and Cuculiformes.


A cheap and quick method of screening potential antimycobacterial agents in the Syrian or golden hamster (Cricetus auratus).


The persistence of abnormal behaviour in monkeys following isolation rearing.


The collie eye anomaly.

The collie eye anomaly.


The collie eye anomaly. Comments.

The collie eye anomaly. Comments.


Eye anomaly of the collie.

Eye anomaly of the collie.


Eye anomaly of the collie. Comments.

Eye anomaly of the collie. Comments.


Anomaly of the collie eye. Comments.

Anomaly of the collie eye. Comments.


Anomaly of the collie eye.

Anomaly of the collie eye.


The collie eye anomaly. Summary and conclusions.


Identification and subcellular localization of the enzymes effecting penetration of the zona pellucida by rabbit spermatozoa.


Carbohydrate metabolism of myometrium from the pregnant rhesus monkey.


Pain-relieving drugs in angina.

Pain-relieving drugs in angina.


Temperature sensitive conditional mutants of monkey kidney cells.


Induction of myxedema by iodide in patients euthyroid after radioiodine or surgical treatment of diffuse toxic goiter.


Mycoplasma mycoides transplacental transfer in cattle.


Ethambutol treatment of tuberculosis in a controlled trial.


Brain damage by asphyxia at birth.

Brain damage by asphyxia at birth.


Short fragments from both complementary strands in the newly replicated DNA of bacteriophage SPP-1. Bacillus subtilis bacteriophage SPP-I has separable complementary DNA strands. Fragments of nascent DNA isolated a very short time after phage infection show that these short chains are complementary to both phage DNA strands, as observed by hybridization techniques.


Ocular abnormalities associated with first and second arch syndromes.


Structural studies on penicillin derivatives. 3. Rearrangement and fragmentation of penicillin V.


Response to Pekin and mallard ducks and Canada geese to experimental infection with duck plague virus.


Utilization review.

Utilization review.


A versatile method of radial immunodiffusion assay employing microquantities of antiserum.


The development of the defibrillator.

The development of the defibrillator.


Survival of a patient with spontaneous lactic acidosis.


Green monkey disease ("Marburg virus" disease): a new zoonosis.


Penetration of thymine-starved bacterial DNA during transformation of B. subtilis 168 T-.


Coarse and fine control of citrate synthase from Bacillus subtilis.


The possible role of histamine in the coritsone induced gastric acid hypersecretion of the guinea-pig. 1. The effects of cortisone treatment combined with agents influencing histamine metabolism were studied on the free and total acid output in pylorus ligated guinea-pigs.2. It was found that reduction in the synthesis of histamine produced by the administration of alpha-methylDOPA significantly inhibited the acid response to cortisone.3. Enhancement of the oxidative deamination of histamine brought about by the administration of diamine oxidase or heparin inhibited cortisone induced acid hypersecretion significantly.4. Inhibition of the oxidative deamination of histamine by aminoguanidine and iproniazid resulted in a significant increase of the cortisone induced acid hypersecretory response.5. Inhibition of the methylation of histamine by chlorpromazine or 1,4-methylhistamine inhibited the cortisone induced hypersecretory response significantly.6. Studies with labelled histamine indicated that cortisone increases the sequestration of histamine to the stomach.


Plasma diamine oxidase activity in humans and marmosets.


Antigenic structure of connective tissue.


Cytotoxic lymphoid cells and antibodies from guinea pigs immunized with tubercle bacilli.


Antigens and allergens of fish.

Antigens and allergens of fish.


The influence of various adjuvants on the metabolism of phospholipids in macrophages.


Serological adhesion in chicken and mammalian cultures with human erythrocytes of different reactivity.


The release of guinea pig liver histaminase and some of its properties.


Mechanism of histaminase liberation in guinea pig anaphylaxis.


Factors contributing to occasional failures in demonstration of pemphigus antibodies by the immunofluorescence test.


Farmer's lung in a 10-year-old girl.

Farmer's lung in a 10-year-old girl.


Some essentials in a national program for child health.


Teflon tubing in chronic arterial catheterization of monkeys.


Placental function tests: a review of tests showing promise but not yet established.


Jejunal dyskinesia: case report and review of the literature.


The isolation of Brucella abortus from a waterbuck (Kobus ellipsiprymnus).


Action of staphylococcal alpha toxin on arterial smooth muscle.


Phenol oxidases produced by mutant and wild-type strains of Aspergillus nidulans.


Structural changes in scrapieaffected brain.


Purification and properties of alpha-D-mannosidase from rat epididymis. 1. alpha-d-Mannosidase from rat epididymis was purified 300-fold. beta-N-Acetyl-glucosaminidase and beta-galactosidase were removed from the preparation by treatment with pyridine. Zn(2+) was added during the purification to stabilize the alpha-mannosidase. 2. Mammalian alpha-mannosidase is most stable at pH6. At lower pH values it undergoes reversible spontaneous inactivation. The enzyme is also subject to irreversible inactivation, which is delayed by the addition of albumin. 3. Reversible inactivation of alpha-mannosidase is accelerated by EDTA and reversed or prevented by Zn(2+). Other cations, such as Co(2+), Cd(2+) and Cu(2+), accelerate inactivation and the action of a toxic cation can be prevented by Zn(2+) or by EDTA in suitable concentration. 4. The enzyme is stabilized by substrate and neither Zn(2+), EDTA nor a toxic cation has more than a small effect in the assay of an untreated preparation. The addition of Zn(2+) is necessary, however, for a constant rate of hydrolysis during prolonged incubation of the enzyme with substrate. In an EDTA-treated preparation, Zn(2+) reactivates the enzyme during the assay. 5. Evidence is presented that alpha-mannosidase is a dissociable Zn(2+)-protein complex, in which Zn(2+) is essential for enzyme activity.


The size and intracellular location of the scrapie agent.


The chemical composition of the lipopolyacarideof Pseudomonas aeruginosa. 1. Lipopolysaccharide was isolated from both cell walls and acetone-dried whole cells of Pseudomonas aeruginosa (N.C.T.C. 1999). 2. Closely similar products are obtained, although that from whole cells cannot be completely freed from small amounts (2-7%) of residual nucleic acids. 3. The lipid moiety (23-33%) has a similar amino sugar backbone to that of lipids of enterobacterial lipopolysaccharides, but contains different hydroxy acids (2- and 3-hydroxydodecanoic acid and 3-hydroxydecanoic acid). 3-Hydroxytetradecanoic acid is absent, and 3-hydroxydodecanoic acid is the main N-acylating acid. No clear evidence permitting a distinction between the possibilities that phosphodiester or glycosidic linkages exist between the glucosamine residues was obtained. 4. Identifiable sugars (glucose, rhamnose, 3-deoxy-2-octulonic acid and heptose) account for less than 20% of the lipopolysaccharide, and alanine, galactosamine and fucosamine are apparently components of the polysaccharide moiety. 5. The polysaccharide moiety is unusual in that it is not readily obtained from the lipopolysaccharide by treatment with dilute acetic acid, which does, however, solubilize much of the phosphorus of the lipopolysaccharide. 6. The ;polysaccharide' fraction (approx. 21%) obtained by treatment with dilute acetic acid contains only a small proportion of the total polysaccharide components, and in one case only 45% of the fraction was accountable for in terms of identifiable components. 7. Evidence suggests that unidentified nitrogenous components are concentrated in the residual material after removal of both the lipid and the ;polysaccharide' fraction from the lipopolysaccharide.


The chemical nature of the scrapie agent.


Cortical response antigens released at fertilization from sea urchin eggs and their relation to antigens of the jelly coat.


Osmoregulation in a marine ciliate, Miamiensis avidus. II. Regulation of intracellular free amino acids.


Swine fever: the interaction of research and control measures.


Grand rounds: university hospital. Diabetic acidosis.


Autoantibodies in haemoglobin in cattle.


Diagnosis of photosensitization reactions by the Scotch Tape provocative patch test.


The formation of the primate nail plate. An autoradiographic study in squirrel monkey.


The skin surface lipids of man compared with those of eighteen species of animals.


The identification of phototoxic drugs by human assay.


Antimicrobial activity of dimethyl sulfoxide against Escherichia coli, Pseudomonas aeruginosa, and Bacillus megaterium.


Chemotherapy of tuberculosis. IX. Synthesis and screening of new thiazolyl thiocarbanilides.


The metabolic fate of the pteridine diuretic, Wy-5256.


Anti-lymphocytic antibody and autoimmune disease: a review.


Simple assay for determination of carbonic anhydrase activity.


The practical significance of recent caries research.


Ordinal position in sibship and mother's Rh stus among psychological clinic patients.


Extensive muscle necrosis after long-term treatment with aminocaproic acid (EACA) in a case of hereditary periodic edema.


Rh hemolytic disease in ABO-incompatible offspring. Observations on the protective effect of heterospecific pregnancy.


Amniotic fluid lactic dehydrogenase in Rh-sensitized pregnancy.


Intrauterine catheterization of ureters of primate fetus.


Neuropsychological deficits in children of diabetic mothers. A report from the Collaborative Sdy of Cerebral Palsy.


Electron microscopy of an adenovirus associated virus.


Interaction of kanchanomycin with nucleic acids. II. Optical rotatory dispersion and circular dichroism.


Dissemination and immunogenicity of live TRIC agent in baboons after parenteral injection. II. Experiments with a 'slow-killing' strain.


Some experiments relating to artificial immunity in enzootic pneumonia of pigs.


Control of cross-infection in an intensive care unit.


Antigenic analysis of vibrio culture filtrate and vaccine El Tor vibrio.


Experimental infection with the virus of lymphocytic choriomeningitis of X-ray-irradiated mice.


Tuberculostatic effect of gentamicin in vitro.


Neutron therapy of cancer: physical aspects.


The acute inhalation toxicity of monomethylhydrazine vapor. AMRL-TR-68-169.


Beta-aminoisobutyric acid excretion in Macaca mulatta primates. SAM-TR-69-6.


Retinal lesion and visual acuity in the rhesus monkey (Macaca mulatta). ARL-TR-69-3.


Auditory sensitivity in the rhesus (Macaca mulatta) and the white- throated capuchin (Cebus capuchinus) monkeys: a comparison of three techniques. ARL-TR-68-14.


Maximum rate kinetic analysis applied to enzyme assay data. Rep No. 564.


The meaning of preoperative cultures in ophthalmology.


Uterine alkaline and acid phosphatase activity in the rhesus monkey treated with synthetic estrogen.


The free amino acids of amniotic fluid during pregnancy of the rhesus monkey.


Uterine contractility during altered vascular pressure in the castrated monkey.


Blood pressure responses to long-term avoidance schedules in the restrained rhesus monkey.


Intra-uterine death from congenital Chagas' disease in laboratory-bred marmosets (Saguinus fuscicollis lagonotus).


Arbovirus studies in the Ohio-Mississippi Basin, 1964-1967. VI. Mermet: A Simbu-group arbovirus.


Serologic diagnosis of Colorado tick fever. A comparison of complement-fixation, immunofluorescence, and plaque-reduction methods.


A preliminary report on the technique of extracting niacin from Mycobacterium tuberculosis cultured on 7H10 agar.


Pulonary edema of high altitude. I. Production of pulmonary edema in animals under conditions of simulated high altitude.


"Battey" disease in Dublin, Georgia.

"Battey" disease in Dublin, Georgia.


Giant muscular hypertrophy of the esophagus.