Problems of specificity of radioimmunoassay of luteinizing hormone.


The oestrogenic potency in the mouse of several substances closely related to diethylstilboestrol and meso-hexoestrol.


Pituitary factors affecting androgen production by the foetal adrenal.


The uptake and intracellular distribution of (1,2-3H)progesterone by human endometrium.


Evidence for suppression of oestrogen output as the mechanism of action of progestational steroids on body weight in female rats.


A new assay for oxytocinase: studies in vivo and in vitro.


The effect of naturally occurring amines on pregnancy in mice.


Evaluation of a competitive protein-binding method for determining progesterone in peripheral plasma.


Effect of thyroid hormone administration on the incorporation of (1-14C) leucine into protein of rat pituitary and hypothalamas during development.


The effect of oxytocin on sodium excretion.


A reference preparation for the study of active substances in the caudal neurosecretory system of teleosts.


A radioimmunoassay for calcitonin M.

A radioimmunoassay for calcitonin M.


Measurement of calcitonin secretion rate in the goose.


A new semi-automated method for the estimation of total urinary oestrogens in pregnancy.


Biochemical studies on four patients with medullary carcinoma of the thyroid.


Calcium homeostasis in mature rats and their offspring.


The ultrastructure of the thyroid of the hagfish, Myxine glutinosa.


Oestriol in amniotic fluid. Variation in concentration over short periods in the same patients.


Effects of oestrogens on serum glycoproteins.


The ratio of 17 alpha- to 17 beta-androstenediol in the foetus, infant and adult.


Electron microscopic studies of Rubella virus.


Biological characterization of structural components of Adenovirus type 12.


The envelope of vaccinia and orf viruses: an electron-cytochemical investigation.


Effect of temperature on the multiplication of an Influenza virus.


Isolation and characterization of Streptococcus agalactiae bacteriophage.


Bacilliform particles associated with mottled dwarf of eggplant (Solanum melongena L.).


Granuloma inguinale: an ultrastructural study of Calymmatobacterium granulomatis.


Variability in the chemical composition of human skin surface lipids.


Ultrastructure of three types of epidermal dendritic cells in hairless mice.


Brocresine, a histidine decarboxylase inhibitor, in chronic urticaria.


In vitro DNA synthesis in chick embryonic skin: a sensitive indicator for testing mitotic stimulatory or inhibitory actions.


Chronic maintenance dialysis for patients with end-stage renal disease.


A novel cellular reaction caused by tinea versicolor: extracellular glycogen deposits.


Early recognition and prompt evaluation of spinal deformity.


Hypertrophy of the ileocecal valve.

Hypertrophy of the ileocecal valve.


New medical curriculum at U. of I.

New medical curriculum at U. of I.


One year's experience in a community hospital coronary-care unit.


Various local efforts aid in the conquest of measles.


Heart disease in infancy.

Heart disease in infancy.


Cardiovascular roentgenology: conventional studies.


Changing public-health nursing programs in Iowa.


What type of image does your office present?


Causes and remedies for malnutrition.

Causes and remedies for malnutrition.


A study of lipid components in brain of the 'Jimpy' mouse, a mutant with myelin deficiency.


Second attacks of acute glomerulonephritis.


Cytoplasmic inclusions in juvenile amaurotic idiocy.


Polycythemia in small for gestational age infants.


Changes in plasma growth hormone after a phenylalanine tolerance test in normal and phenylketonuric children.


Studies of the secretion and metabolic effects of human growth hormone in children with glucocorticoid-induced growth retardation.


The diagnostic value of gamma glutamyl transpeptidase in children and adolescents with liver disease.


Variability of the de Lange syndrome: report of 3 cases and genetic analysis of 54 families.


Rape: a complex management problem in the pediatric emergency room.


Transverse myelopathy secondary to injection of penicillin.


Untaxed whiskey and fetal lead exposure.


Salt-losing nephropathy.

Salt-losing nephropathy.


The prevalence of congenital heart disease in United States college freshmen, 1956-1965.


Hereditary orotic aciduria: long-term therapy with uridine and a trial of uracil.


Roentgen examination in the evaluation of the newborn infant with respiratory distress.


Ankylosing spondylitis.

Ankylosing spondylitis.


Effect of esophageal mucosal denervation on the motility of the canine esophagus.


Exchange diffusion in acid-filled gastric pouches.


The relationship of age to canine cerebral metabolic rate.


Maintenance of mitochondrial volume and the effects of phosphate and ATP in producing swelling and shrinking.


Studies on transglycosidation to vitamin B6 by microorganisms. I. Formation of a new vitamin B6 derivative, pyridoxine glucoside, by Sarcina lutea.


Study on the pyridoxal kinase of mouse brain.


Studies on fatty acid esters of flavins. VI. Incorporation of riboflavin part of riboflavin tetrabutyrate-2-14C into flavin nucleotides in the organs of rat.


Studies on transglycosidation to vitamin B6 by microorganisms. II. Chemical structure of pyridoxine glucoside.


Studies on transglycosidation to vitamin B6 by microorganisms. 3. Distribution of activity of pyridoxine glucoside formation in microorganisms.


Studies on vitamin B12 status in blood and tissue of alloxan diabetic animals.


Direct incorporation of orally administered riboflavin tetranicotinate into portal blood of rabbit.


Smallpox vaccination and quarantine: a new exemption policy.


Enzymic hydrolysis of flavin mononucleotide 2',3'-dibutyrate.


Social class and time.

Social class and time.


A tellymyth.

A tellymyth.


Stereophotogrammetry of fluorescein angiographs in ocular biometrics.


Recurrent parotitis and associated calculus, cyst or tumour.


Hair-grafting: a practical possibility.

Hair-grafting: a practical possibility.


A non-fatal case of blue-ringed octopus bite.


Religion and medicine.

Religion and medicine.


Diazepam and awareness.

Diazepam and awareness.


The coronary care unit: a six-month experience in a community hospital.


A project to assist community hospitals in establishing coronary care units.


Retrocaval ureter: a case report and successful repairwith a new surgical technique.


Fee-for-service concept in dire danger.

Fee-for-service concept in dire danger.


The Professional Service Corporation in Michigan.


Muskegon doctors release figures of study of their relationship with Blue Shield.


The 1969 presidential address. Physical therapy and medicine.


Human relationships in administration.

Human relationships in administration.


Development of a research section.

Development of a research section.


A comparison of strength gain between concentric and eccentric contractions.


Nystatin in the treatment of candidiasis.


Q-methodology in rating the clinical competency of physical therapy students: a model.


Case report. A prosthetic thumb.

Case report. A prosthetic thumb.


Relationships between hospital administrators and physical therapists. I. Understanding the administrator.


Drug use in the year 2000 by normal humans.


Synthesis of iboga alkaloids and related compounds.


Chemical studies on indole alkaloids.

Chemical studies on indole alkaloids.


Heterocyclic analogs of biologically active indoles.


Splenectomy on children with thalassemia.


Mortality in infants and children of Indonesian doctors.


Synthesis of some substituted beta-lactams.


Synthesis of compounds related to lysergic acid.


Congenital adrenal hyperplasia.

Congenital adrenal hyperplasia.


The methodology of international studies of the effectiveness of psychotropic drugs--a conference.


Chlorpromazine metabolites and mode of action.


Phenothiazines and phenothiazine analogs.


Drug-induced behavioral states and their antagonists.


Leguminosae alkaloids as new psychotropic drugs.


The indence of congenital malformation in the Dr. Tjiptomangunkusumo, general hospital, Jakarta, 1962-1967.


Benzodiazepines as potential tranquilizers.


Seven-membered heterocyclics of medicinal potential.


Physiological properties of mecromerine.


Intramolecular catalyzed dehydration of pyschotropic agents.


Synthesis of potential psychotropic agents.


Condensed polyheterocycles containing N, S and O.


Synthesis of aromatic compounds.

Synthesis of aromatic compounds.


Use of bioelectric potentials for screening drugs.


Phenothiazine tranquilizers: confomational studies.


Intermediary metabolism of mescaline analogs.


A study of alkoxycarbonium ions related to mescaline.


Charge transfer complexes of tetrazoles.


Drugs with psychopharmaceutical effects.


Microbial production of lysergic acid and psyilocybin.


Psychopharmacological agents: stereochemical studies.


Industrial engineering in vocational rehabilitation. A look to the future.


Operant conditioning in a sheltered workshop. Further data in support of an objective and systematic approach to rehabilitation.


Propulsive gastrointestinal motility after controlled bleeding in the rat.


Exchange circulation of the small intestine on controlled bleeding in the rat.


Effe of vagotomy or atropine on the canine pancreatic response to secretin and pancreozymin.


Variability of the sum of bicarbonate and chloride concentrations in the pancreatic juice of the dog.


Cholinergic effect on the canine pancreatic response to acidification of the duodenum.


Succinic dehydrogenase activity in the gastric mucosa of rats during hyperthermia.


On anxiety and depression.

On anxiety and depression.


Dermatoglyphics.

Dermatoglyphics.


Immune response in vitro: independence of "activated" lymphoid cells. Antibody formation against sheep erythrocytes by mouse spleen cells in vitro requires interactions among antigen-treated macrophages and lymphoid cells in cell culsters for only a finite time. During this critical period of interaction, lymphoid cells become "activated" and thereafter can develop into antibody-producing cells independently of native antigen, macrophages, and cell clusters.


Single chain alkali resistance in hemoglobin Rainier: beta 145 tyrosine-histidine. The mechanism of alkali resistance in hemoglobin Rainier, an adult human hemoglobin variant (beta 145 tyrosinie --> histidinie), has been investigated. Alkali denaturation kinetics, electrophoretic and hybridization study. and ultracentrifuge analysis provided evidence for a monomeric beta Rainiier chain resisting denaturation at alkaline pH. These data provide evidence for a previously unrecognized effect of a single amino acid substitution, that is, the change of the alkali denaturation properties of monomneric chains.


Beta-hydroxybutyrate dehydrogenase: lack in ruminant liver mitochondria. The enizyme D(-)-beta-hydroxybutyrate dehydrogenase has been reported to occur in all mitochondria isolated from mammalian tissues and is especially high in liver. By contrast only very low concentration of the enzyme is detectable in mitachondria from bovine and sheep liver. The amount of the enzyme in mitochondria from other ruminant tissues such as kidney and heart are comparable with those from mammalian sources. The verv low activity of beta-hydroxybutyrate dehydrogenase is not referable to the iniability of substrates to penetrate milochondria or to decreased electron transfer activity but rather to a lack of the enzyne in ruminant liver mitochondria.


Alpha-naphthoflavone: an inhibitor of hydrocarbon cytotoxicity and microsomal hydroxylase. Alpha-naphthoflavone inhibits the metabolism of 3,4-benzopyrene and 7,12-dimethylbenz(a)anthracene in hamster enlbryo cell cultures and protects the cells against the inhibition of cell multiplication by these carcinogens. Alphla-nalphthoflavone also inhibits the aryl hydrocarbon hydroxylase activity in homogenates of induced hamster embryo cells and in liver microsomes from rats previously treated with polycyclic aromatic hydrocarbons, but not in microsomes from control rats.


Ribonucleic acid metabolism of a single neuron: correlation with electrical activity. The giant neuron of the abdominal ganglion of Aplysia californica incorporates tritiated uridine into RNA at a constant rate at rest. This rate incrceases under synaptic stimulation, the increase being directly proportional to the number of action potentials produced by the neuron. Multineuronal samples from stimulated ganglia faiiled to show an increase in incorporation.


Postpartum psychiatric reactions: time of onset and sex ratio of newborns. There is no predominance of male infants born to women who develop schizophrenic symptomns with in 1 month after delivery. Moreover, there is no difference in the sex ratio of infants born to schizophrenic women whose onset of symptoms occurred within the first 10 days postpartum, which is the period of rapid hormonal changes that is most likely to reflect postnatal influences of fetal sex.


Neuron function inferred from behavioral and electrophysiological extimates of refractory period. The refrcactory period of neurons mediating an electrically elicited behavior (self-stimulation) was estimated by varying the intrapair pulse separation in a stimulating train made up of pulse pairs and measuring the intensity of the elicited behavior. Two neuronal systems with different refractory periods were indicated. Single-unit recording in acute preparations stimulated through self-stimulation electrodes revealed primarily two classes of units. Each class gave refractory period estimates characteristic of one of the behaviorally indicated systems. The experiments illustrate a technique for establishing functional relations between single units in the brain and gross behavior.


Elution of glomerular bound antibodies in experimental streptococcal glomerulonephritis. lmmnunoglobulin G, eluted from glomeruli of rats with streptococcal glomerulonephritis, reacts with type 12 M protein of the streptococcus but not with other streptococcal or renal antigens. Thtrefore, this disease may be madiated by fixation of antigen-antibody complexes consisting of streptococcal M protein and type-specific antibody.


Environment: focus on DDT, the"uninvited additive".


Herbicides: order on 2,4,5-T issued at unusually high level.


Solvent effect on the autoxidation of L-ascorbic acid.


The structure and synthesis of pongachromene, a new component of Pongamia glabra.


Selective esterifications and acyl rearrangements in vitamin B6.


Modified steroid hormones--LI. Application of the Vilsmeier reaction to 11-beta-hydroxy steroids.


Geographical variations in the incidence of sarcoidosis in Great Britain: a comparative study of four areas. A report to the Research Committee of the British Thoracic and Tuberculosis Association.


Patient's preference of drugs in the treatment of tuberculosis.


Changing pattern of tuberculosis notifications in Birmingham.


Nursing today.

Nursing today.


Rifampicin.

Rifampicin.


Acute scrotal swelling in children.

Acute scrotal swelling in children.


Pneumoperitoneum in infancy.

Pneumoperitoneum in infancy.


Rupture of pulmonary artery in pulmonary hypertension.


SClinicopathological conference ... lung lesion and focal neurologic signs.


Primary amoebic meningoencephalitis.

Primary amoebic meningoencephalitis.


Chronic ulcerative colitis and Crohn's disease of the colon.


Comparative study of glutamic oxalacetic transaminase and creatine phosphokinase values in the differential diagnosis of myocardial infarction.


Enzyme changes in myocardial infarction.


Diverticulitis.

Diverticulitis.


Some comments on the new anti-arrhythmic drugs.


Genetic variation in the orientation of nuclear spindles during the development of asci in Neurospora.


Influence of dextran upon renal blood flow in normovolemic and hypovolemic animals treated with adrenergic blocking agents.


Kinetics of monovalent and divalent anion distribution in hemorrhagic hypotension and hypoxemia.


Effects of anesthesia on pancreatic exocrine secretion.


The treatment of pancreatic exocrine insufficiency. 3. The effects of pancreatic ductal ligation and oral pancreatic enzyme supplements on fecal lipid excretion in the dog.


Pancreatic blood flow and its relationship to insulin secretion during extracorporeal circulation.


Pancreaticoduodenectomy for severe trauma to the head of the pancreas and the associated structures: report of three cases.


Gastric ulcers following vagotomy in swine.


Esophageal achalasia following lye ingestion.


Penetrating injuries of the liver in Vietnam.


The influence of intravenous fluid administration on postoperative urinary water and electrolyte excretion in thoracic surgical patients.


Lung lipid metabolism and surface tension phenomena following pulmonary embolism.


Central venous aneurysms and arteriovenous fistulas.


Extraosseous multiple myeloma: a cause of intestinal obstruction.


Superior mesenteric arteriovenous fistula.


Bilateral acinic cell carcinoma of the parotid gland.


Abdominal aortic aneurysm and horseshoe kidney.


Road traffic accidents. The therapeutic vacuum.


Pitfalls in diagnosis and treatment after serious injury.


Who looks after head injuries?

Who looks after head injuries?


Maxillofacial injuries.

Maxillofacial injuries.


Undernutrition and surgery in regional ileitis.


Gastric cancer simulating benign gastric ulcer.


Perineal wound healing in ulcerative colitis.


The prevention of recurrent pulmonary embolism.


Influence of environmental light and melatonin upon mammary tumour induction.


The effect of jejunal segment interposition on post-gastrectomy malabsorption in dogs.


Cytotoxic penetration and concentration in human malignant tumours.


Gall-bladder function after vagotomy.

Gall-bladder function after vagotomy.


Breast cancer symposium. Points in the practical management of breast cancer.


Breast cancer symposium. Points in the practical management of breast cancer. How and why does one "stage" a patient?


Breast cancer symposium. Points in the practical management of breast cancer. Are physical methods of diagnosis of value?


Taking it that removal of the breast is required for the primary tumour, how should one treat the axillary nodes--by surgery, by radiotherapy, or by both?


What is the place of hormones in prophylaxis after mastectomy? Methods of prophylaxis of recurrence of breast cancer are discussed.  It has been demonstrated that if small doses of estrogens are administered to a patient who has overt or occult breast cancer the tumor can be encouraged to grow more quickly.  Patients who have undergone mastectomy should be advised not to take estrogens in any form.  If a patient becomes pregnant after a mastectomy, any latent carcinoma cells may be encouraged to grow and metastases will appear sooner than would otherwise have been the case, but the pregnancy makes no difference as to whether the patient  is cured.  The patient might be advised to avoid pregnancy for a few years at any rate.  Although there is some concern about such patients lactating, lactation should not be suppressed by estrogens.  It has been known for many years that if a premenopausal woman is castrated after mastectomy her survival may be slightly increased, and the time of recurrence of her tumor postponed.  Castration is not a cure but it may delay the appearance of metastases.  There are some factors against castration and the place of oophorectomy at the time of mastectomy is uncertain.  Cole found that patients who had an artificial menopause delayed until recurrence had occurred fared as well in terms of ultimate survival as those who had it performed at the time of masectomy.


The role of myo-inositol in metabolic control. 3. The effects of carbon sources on myo-inositol deficiency of Saccharomyces carlsbergensis 4228 (ATCC 9080).


Biopharmaceutical studies on guiacol glycerol ether and related compounds. 3. Metabolites of guaiacol glycerol ether and its mononicotinate. 1.


Copper-catalyzed oxidation of ascorbic acid.


Absorption and excretion of drugs. XLI. Studies on the gastrointestinal absorption of 2-pyridine aldoxime methiodide and its derivatives. 1.


Studies on the glucaric acid pathway in the metabolism of D-glucuronic acid in mammals. 3. Determination of D-glucaric acid in serum, with special reference to its level after administration of d-glucuronolactone and D-glucarolactones to man.


Isolation and characterization of leupeptins produced by Actinomycetes.


Studies on monoterpene glucosides. VII. Iridoid glucosides of Paederia scandens.


Polysaccharides in lichens and fungi. 3. Further investigation on the structures and the antitumor activity of the polysaccharides from Gyrophora esculenta Miyoshi and Lasallia papulosa Llano.


Studies on monoterpene glucosides. 8. Artefacts formed during extraction of asperuloside and paederoside.


Inhibition of the copper-catalyzed oxidation of ascorbic acid by 1,10-phenanthroline.


Synthesis of ecdysterone.

Synthesis of ecdysterone.


Isolation of insect-moulting substances from Blechnum amabile and Blechum niponicum.


Haemoglobin synthesis in vitro by erythrocytes from Xenopus laevis.


The effect of reserpine on the vesicle content of Helix aspersa cerebral ganglia.


Cultivation of Trypanosoma lewisi in a dialysate medium. I. Amino acid alterations during growth.


A comparative study of histidase activity in amphibian, avian, reptilian and mammalian epidermis.


The synthesis of dopamine from DOPA in the ganglia of Mercenaria mercenaria (Mollusca, Pelecypoda).


The role of beta-alanyl-L-tyrosine (sarcophagine) in puparium formation in the fleshfly Sarcophaga bullata.


Evidence for efference copy for eye movements in fish.


Tetrahydrofolate cofactors in the housefly Musca domestica.


Effect of noradrenaline on the plasma free fatty acid and glucose levels in Gallus domesticus.


Diuretic responses to electrolyte loads in four strains of mice.


Biosynthesis of human placental proteins and human placental lactogen (HPL) in vitro. I. Identification of 3H-labeled HPL.


Biosynthesis of human placental proteins and human placental lactogen (HPL) in vitro. II. Dynamic studies of normal term placentas.


Effect of endometrial phase, ovariectomy, estradiol and progesterone on uterine protocollagen hydroxylase.


Effect of sodium deprivation on the tissue uptake and metabolism of 3H-1,2-d-aldosterone in rats.


Aminoacyl and dipeptidyl arylamidases (aminopeptidases) of the pituitary gland as related to function.


Effects of magnesium on calcium and phosphate metabolism in parathyroidectomized rats.


"Insulin-like" action of oxytocin: evidence for separate oxytocin-sensitive and insulin-sensitive systems in fat cells.


Autoradiographic analysis of estradiol uptake in the brain and pituitary of the female rat.


Thyroid hormones on amino acid and protein metabolism. I. Concentration and composition of free amino acids in blood plasma of the rat.


Effect of long-term administration of porcine calcitonin in the development of dietary osteoporosis in cats.


Nonsurgical transfer of eggs through the cervix in rats.


Environmental lighting and neuroendocrine function: relationship between spectrum of light source and gonadal growth.


Cytophotometric study of nuclear proteins and nucleic acids in parenchymatous tissue of the orchid embryo.


Evidence for a small pool of immunocompetent cells in the mouse thymus.


Requirements of ribonucleic acid synthesis for the formation of salivary gland specific proteins in larval Chironomus tentans.


Character changes induced by heterologous nuclei in amoeba heterokary- ons.


Estradiol-induced mitotic delay in HeLa cells: reversal by calcium chloride and putrescine.


Isolation and characterisation of polysomes and polysomal RNA from radish leaves.


Monodisperse suspensions of synchronous cells in a defined medium of unchanging composition.


DNA polymerase activity of cultured normal and leukemic lymphocytes. Response to phytohemagglutinin.


The synthesis of DNA in synchronized cultures of Tetrahymena pyriformis GL.


Sea urchin nuclear DNA polymerase. I. Localization in nuclei during rapid DNA synthesis.


Species and tissue specificity of very lysine-rich and serine-rich histones.


Microextensions on Chang's liver cells as observed throughout their division cycle.


Mycoplasma contamination of cell cultures: interference with 32P-labelling pattern of RNA.


A structural protein extracted from the trichocyst of Paramecium aurelia.


Karyological and morphological characteristics of human diploid cell strain WI-38 infected with mycoplasmas.


The uptake of bases and their incorporation into RNA during the cell cycle of Schizosaccharomyces pombe in normal growth and after a step-down.


Preliminary estimates of the mean duration of the S-phase in the opossum, Didelphis virginiana.


The effect of aldosterone on the isolated frog skin epithelium (R. temporaria).


Early stages in the reaggregation of embryonic chick neural retina cells.


Perforations of Descement's membrane on the periphery of the pig cornea as a basis of rudimentary praestomata aquosa.


Diametric effects of histones and the non-histone proteins on DNA replication in vitro.


Influence of the long-term, continuous bending on the bone. An experimental study on the tibia of the rabbit.


Electron microscopic observations on the filaments in the trophoblast of the human placenta.


Pia mater and membrana limitans gliae superficialis in human ontogenesis.


Characterization of the postsynaptic structures of the brain.


Ontogenesis of the layer of Mm. contrahentes in the human foot.


Combined heat-radiation treatments to control storage rots of spadona pears.


Oxygen flask combustion analysis of 14C and 3H: description of method and apparatus and summary of experience, with emphasis on analysis of low levels of activity.


Experience with purity tests of 113Sn-113mIn generators.


Triton X-100 scintillant for counting calcium-45 in biological fluids.


Hazards in the use of liquefied gas refrigerants in irradiation experiments.


Radioarsenic labelling of chromium compounds.


Autoradiographic detection of iodine-131 using polaroid film.


Inadequacy of an external standard in correcting for quenching in liquid scintillation counting of tritiated water in biological material.


Difference between fasting and second-hour blood sugar level: its clinical significance.


Serum magnesium in hepatic disorders.

Serum magnesium in hepatic disorders.


Additive effect of intravenous and intrasplenic pituitrin in portal hypertension.


Chronic cor pulmonale in pulmonary tuberculosis.


Treatment of anorexia nervosa with chlorpromazine and modified insulin therapy.


Tuberose sclerosis--a report of three cases in a family.


Coexisting pulmonary tuberculosis and primary bronchial carcinoma.


Experiences with D.C. countershock treatment: observations on factors influencing conversion and relapse of atrial arrhythmias.


Transaminase activity of ascitic fluid.

Transaminase activity of ascitic fluid.


The effect of emetine on the electrocardiogram and the serum transaminases.


Cranio--vertebral anomalies with neurological deficit (a study of nine cases).


A clinical and carotid angiographic study of cerebrovascular occlusions.


Functioning tumor of organ of Zuckerkandl (report of a case with various unusual features).


Treatment of hyperthyroidism with radioiodine: some pertinent observations.


Osteolytic areas and pathological fractures in acute leukemia in an adult.


Degos syndrome.

Degos syndrome.


Effect of stable iodine on thyroid iodine release.


Excretion of urinary free amino acids in Cushing's syndrome.


In vivo studies on progesterone metabolism by human skin.


Studies on steroid conjugates. VI. Quantitative paper chromatography of urinary corticosteroids in essential hypertension.


Regulation of the secondary antibody response in vitro. Enhancement by actinomycin D and inhibition by a macromolecular product of stimulated lymph node cultures. Two opposite effects of actinomycin D on antibody synthesis have been studied in organ cultures of rabbit lymph node fragments. These cultures were prepared from previously primed rabbits and stimulated with antigen(s) on day 0 to yield a secondary response, whose inductive phase extended to about day 9 and whose productive phase may last for several months in the serum-free medium described here. Concentrations of actinomycin D above 0.01 microM (0.012 microg/ml) produce inhibition of antibody synthesis during both phases of the response. However, antibody synthesis is about 10 times more sensitive to inhibition by this drug when it is added during the inductive phase than during the productive phase. During the latter phase, synthesis is more rapidly terminated as the drug level approaches 10 /microM (12.5 microg/ml). At this level the 50% synthesis time is about 2.8 hr, which is identical with that found when 5-10 microM puromycin is added to the medium of parallel cultures. Transient enhancement of antibody synthesis is frequently produced by a brief exposure to low levels of actinomycin D (generally less than 0.01 microM). Enhancement appears in precise temporal association with actinomycin pulses added for 2 days or less only between days 6 and 16. This apparent enhancement of antibody synthesis resembles the increased enzyme synthesis described by Garren et al. (6) and led to a search for an antibody-inhibitory material (AIM) whose synthesis might be stopped preferentially by low levels of the drug. Stimulated lymph node cultures produce between days 6 and 15 a nondialyzable material which inhibits antibody synthesis during the productive phase of heterologous antigen-antibody culture systems. Just as enhancement with low levels of actinomycin D appears within 2 hr after the drug has been added to cultures, so inhibition occurs within 4 hr of adding AIM to cultures during their productive phase. These observations suggest that AIM is analogous to the translational "repressor" postulated by Garren et al. (6). AIM has relevance in two areas of immunology: (a) it may be the explanation for many examples of antigenic competition, and (b) it may represent a normal control mechanism for the productive phase.


Endemic goiter with hypothyroidism in three generations.


Antigen recognition: in vitro studies on the specificity of the cellular immune response. Studies of the immunochemical specificity of antigen-induced thymidine-2-(14)C incorporation in lymph node cells obtained from animals immunized to a series of closely related alpha-DNP-oligolysines, epsilon-DNP-oligolysines, and oligolysines have shown that the sensitized cell exhibits an extraordinary degree of specificity for antigen. The sensitized cell is maximally stimulated by the homologous immunizing antigen and can discriminate among compounds which differ from one another only in the position of a dinitrophenyl group or D-lysine residue on an identical oligolysine backbone. These studies support the view that the immunogen is not degraded prior to the induction of the immune response, and that the majority of cells produced as a consequence of immunization have stereospecific antigen receptors for the DNP-oligolysine used to induce the response; a smaller and more variably sized population of cells is produced with receptors specific for the oligolysine portion of the immunizing antigen. When specifically sensitized lymph node cell cultures are stimulated in vitro by heterologous DNP-oligolysines, the oligolysine- and not the DNP-oligolysine-sensitive population of cells appears to play a crucial role in the specificity of such cross-reactions. It is concluded from these studies that the antigen receptor on the sensitized lymph node cell differs in both kind and degree from conventional antibody. The chemical nature of the receptor and the means by which this receptor reacts with antigen to initiate the biosynthetic or proliferative cellular immune response still remain undefined.


Electron microscopic studies on streptococci. I. M antigen. The presence of M antigens on group A streptococci is associated with hairlike fimbriae that cover the surface of the streptococcal cell wall and are demonstrable by electron microscopy. These fimbriae also may be associated with R antigen. Like M protein, the surface fimbriae are destroyed by trypsin treatment and reappear when "trypsinized" streptococci are reincubated in fresh, trypsin-free broth. Ferritin-conjugated, type-specific antibodies localize on homologous M+ cells in a pattern suggestive of several M antigenic sites along the length of individual surface fimbria. The M-associated fimbriae remain on the residual cell wall after removal of the bulk of group-specific polysaccharide through nitrous acid extraction. This suggests attachment of the fimbriae to the mucopeptide and minor polysaccharide components remaining in the nitrous acid-extracted wall. The pattern of localization of ferritin-conjugated antibodies on homologous streptococci before and after trypsin exposure and upon reincubation of the trypsinized cells in fresh medium suggests the following hypothesis: M antigen is secreted by the cell, is partially excreted through the otherwise intact cell wall, and is bound by the wall so that M protein occupies a peripheral, exposed position on the surfaces of the streptococcal cell wall.


Quantitative investigations of idiotypic antibodies. I. Analysis of precipitating antibody populations. Specifically purified anti-p-azobenzoate antibodies of the IgG class from individual rabbits were used to elicit anti-idiotypic antibodies in recipient rabbits. Allotypes of each donor and recipient were matched. When polymerized antibodies were used for immunization, more than 80% of the recipients responded with the formation of antibodies that precipitated the monomeric donor antibody. Percentages of precipitable molecules in the donor antibody population (D) varied from 4 to 56. As little as 4% was readily detectable by the Ouchterlony method or precipitin test. Specificity of the reaction was tested by double diffusion in agar gel against a panel of purified antibenzoate antibodies from 14 heterologous rabbits and, quantitatively, in three systems by measurement of the extent of coprecipitation of heterologous, radiolabeled antibenzoate antibodies. No cross-reactions were observed. Reactions were shown to be attributable to antibenzoate antibodies in the donor serum, and contributions of allotypic reactions were excluded. In three systems investigated quantitatively, and in one studied qualitatively, two recipients of the same donor antibody produced anti-antibody that reacted with essentially the same subfraction of the donor antibody population. The findings that only a portion of the D population is immunogenic, and that the same subfraction is frequently immunogenic in different recipients, suggest that the immunogenic population comprises a limited number of homogeneous groups of antibody molecules. This is supported by the small number of bands usually observed by the Ouchterlony technique. Quantitative methods of analysis should provide an approach to the study of cell populations producing antibodies of a particular idiotype.


The eighth component of human complement (C8): isolation, characterization, and hemolytic efficiency. A method was described allowing the isolation of the eighth component of complement, C8, from human serum. C8 is an immunochemically definable, heretofore unrecognized serum protein with a molecular weight of approximately 150,000 and an electrophoretic mobility of a gamma(1)-globulin. It may be obtained with 5% yield as a 5000-fold purified, physicochemically and immunochemically homogeneous protein. Under optimal experimental conditions, a few molecules of C8 were found sufficient for lysis of a cell.


Responses to immunization in the thymus of the adult mouse. The rate of synthesis of RNA in the thymus glands of adult mice increased after immunization with sheep red blood cells (SRBC). The specific activity of some fractions of RNA, separated first by density gradient centrifugation and then by polyacrylamide gel electrophoresis, was 16-fold higher on day 3 after immunization than control mice not injected. RNA synthesis in the thymus was inhibited by rabbit anti-mouse thymus serum, injected along with antigen. A material was found in RNA extracts from the thymus glands of mice immunized with SRBC which converted a small proportion of either spleen cells or peritoneal cells from nonimmunized mice to form sheep cell hemolysins. Neither extracts from the glands of nonimmunized mice nor the livers of immunized mice were active. Extracts from the thymus glands of mice immunized with rabbit red blood cells (RRBC) were inactive and activity was destroyed by ribonudease. The residual antigen content was not determined. Biologically active extracts from the thymus had a different electrophoretic mobility from active extracts from the spleen.


Regulation of antibody formation by serum antibody. I. Removal of specific antibody by means of immunoadsorption. Rabbits were injected intravenously with bovine serum albumin (BSA) and bacteriophage T(2) (T(2)). 2-3 wk later, anti-BSA was removed from such animals by a procedure which involved exposure of removed plasma to an immunoadsorbent ((125)I-BSA bound to bromoacetyl cellulose) and return of the adsorbed plasma to the animal. This resulted in removal of the majority of antibody activity to BSA without affecting antibody levels to T(2). 1-2 days later, anti-BSA levels began to rise, and reached peak levels usually 5 days after the removal of antibody. Antibody levels to T(2) did not change. No evidence was obtained that BSA was released from the immunoadsorbent into the circulation of the rabbits. Thus, only trace amounts of radioactivity were released into the plasma; most of the radioactivity was equally coprecipitable with BSA or human gamma globulin and their specific antibodies; the released material was not demonstrated to be immunogenic in primed rabbits; and the released material did not elute with BSA on gel filtration. The results are interpreted as evidence that serum antibody acts as a regulatory mechanism for antibody formation during the conventional antibody response to a metabolizable antigen.


Lasting biological effects of early environmental influences. V. Viability, growth, and longevity. The effects of neonatal influences on the growth and longevity of mice were studied by using animals derived from a highly inbred germfree colony that had been reassociated with a microbial flora free of known pathogens. The size of the animals at weaning time could be conditioned predictably by manipulating the diet of their mothers during gestation and lactation or by shortening or lengthening the period of lactation. A deficient diet during gestation or during lactation decreased the metabolic efficiency of the adult animal, even if it was fed an optimum diet after weaning. The effect was greatest when malnutrition occurred during both pregnancy and lactation. In contrast, an optimum diet during gestation and lactation rendered the animal less susceptible to the depressing effects of nutritional deficiency during adult life. A marked and lasting growth depression could be reproducibly achieved by contaminating newborn mice orally with an unidentified enterovirus. But neonatal infection with enterobacteria or mycobacteria even though severe, did not significantly alter the growth rate. Regardless of its initial cause, the depression of the growth rate during the preweaning period persisted throughout the whole life span of the animals, even when they were placed under optimum sanitary and nutritional conditions after weaning. Agencies (nutritional or infectious) which brought about a depression of whole body weight also affected the absolute and relative sizes of the various organs, especially of the brain. By manipulating neonatal influences, it was possible to produce at will in a given colony of highly inbred mice a family of strikingly different growth curves. This could be done without causing the death of any animal or affecting longevity.


Predicting coping behavior in college. A prospective use of the student-TAT.


Antigenic structure of cell surfaces. An immunoferritin study of the occurrence and topography of H-2' theta, and TL alloantigens on mouse cells. The representation of mouse alloantigens belonging to three systems, H-2, theta and TL, on the surface of cells from thymus, spleen, lymph nodes, and peritoneal cavity, was studied by electron microscopy with ferritin-labeled antibody. As expected from earlier serological data, TL was confined to thymocytes, theta was found on thymocytes and lymphocytes, and H-2 occurred to some extent on all cell types observed. On reticular cells, lymphocytes, plasma cells, and eosinophils, the majority of the cell surface was occupied by H-2; thymocytes had considerably less H-2, and erythrocytes and peritoneal macrophages least of all. In every instance the representation of antigen was discontinuous, the fraction of the cell surface covered being characteristic both of the antigen and of the type of cell. H-2 and theta provide a striking example of this; H-2 is present in far higher amounts on lymphocytes than on thymocytes, whereas the converse is true of theta. Within areas positive for H-2 or theta, protuberances of the surface membrane were often antigen-negative. A better definition of cell surface structure, gained from studies such as this, is necessary for further inquiry into how the cell surface is assembled, and into selective gene action in relation to cellular differentiation.


Attitudes of discharged mental patients toward their hospital experiences. A preliminary study.


Sex differences in the relationship between premorbid asociality and posthospital outcome.


Self-derogation and psychosocial adjustment.


Sensitivity of single neurons in auditory cortex of cat to binaural tonal stimulation; effects of varying interaural time and intensity.


Discharge properties of Purkinje cells recorded on single and double microelectrodes.


Variability of cortical auditory evoked response.


Response of cutaneous sensory units with unmyelinated fibers to noxious stimuli.


Antidromic invasion of Purkinje cells in frog cerebellum.


Excitatory responses of geniculate neurons of the cat.


The crustacean axon. I. Metabolic properties: ATPase activity, calcium binding, and bioelectric correlations.


Responses of inferior colliculus neurons in the cat to binaural acoustic stimuli having wide-band spectra.


Calcium and potassium currents of the membrane of a barnacle muscle fibre in relation to the calcium spike. 1. The behaviour of membrane currents in a giant muscle fibre of a certain barnacle Balanus nubilus, Darwin, was studied by using voltage clamp technique after treating fibres with a Ca-chelating internal solution.2. Membrane currents can be classified into an early transient current and a late outward current.3. The early transient current can be considered a sum of an inward and an outward component of different properties.4. A conditioning depolarization suppresses the inward component but not the outward component.5. Procaine suppresses the outward component and Co ions suppress the inward component.6. Changes in the external Ca ion concentration alter the inward component but not the outward component.7. The inward component is considered to be carried by Ca ions and the outward component mainly by K ions.8. The outward component of the early current is similar to the current expected from the resting membrane conductance.9. It is concluded that the early conductance increase of the barnacle muscle fibre membrane occurs only to Ca ions but not to K ions, although the membrane potential at the peak of spike is determined by both ions.


Structure and some contractile properties of fast and slow muscles of the chicken. 1. A comparative study has been made of the structure of the posterior and anterior latissimus dorsi muscles (P.L.D. and A.L.D.) of the chicken. Some of the contractile properties of these muscles, particularly of the A.L.D. muscle, have also been examined.2. In confirmation of earlier work, it is found that structural differences between these two muscles mainly reside in the organization of the T-system and the sarcoplasmic reticulum.3. In the P.L.D. muscle the T-system and sarcoplasmic reticulum are tubular networks of regular pattern, with frequent regions of contact between them in the form of the familiar triads. As described earlier, two series of triads are present in each sarcomere, near the level of the A-I boundary.4. The presence in the A.L.D. muscle of both T-system and sarcoplasmic reticulum has been established here by the combined use of electron microscopic and histochemical techniques. The appearance of these structures is considerably less regular in this than in the P.L.D. muscle, and the area of contact between them relatively small: in terms of contact area per sarcomere, about 15% or less of that in the P.L.D. muscle. Regions of contact are mainly in the form of dyads, but a few triads are also present.5. Contractures of the A.L.D. muscle in high K fluids have been recorded and their modification due to variation of the external concentration of Ca, K and Mg examined. The K contracture consists of two phases: an initial transient and a later maintained phase. The response of these phases to variation in the external medium is markedly different.6. Application of Ca-free fluids causes contracture tension of the late phase to subside within a few minutes whilst that of the initial phase declines completely only after several hours. High Mg concentrations depress the later but not the early phase of contracture.7. Tentative interpretations of both the morphological and physiological differences described have been proposed.


The rate of osmotic influx of water by flexible and inflexible erythrocytes. 1. The rate of osmotic influx of water into flexible and hardened erythrocytes has been studied using a stopped-flow rapid-reaction method, and diluting the cells 1:20 in hypotonic media.2. Neither the initial rate of swelling nor the time to the onset of haemolysis were changed after making the erythrocytes inflexible by adding formaldehyde or by heating at 48 degrees C for 30 min.3. From a plot of the initial rate of uptake with hypotonicity, the permeability coefficient k can be calculated as 0.098 mu(3)/mu(2). sec atm (+/- 10%) at 20.5 degrees C.4. The value of k calculated from the time to the onset of haemolysis is 0.28 mu(3)/mu(2) sec atm. This suggests individual cells may differ over a wide range of permeability.5. The results are consistent with the pores being at fixed sites in the membrane and unaffected by flexing of the cell.


Mechanical properties of glycerinated fibres from the tymbal muscles of a Brazilian cicada. 1. Some of the properties of glycerinated fibres from the synchronous tymbal muscles of the cicada Fidicina rana have been investigated.2. In the presence of suitable concentrations of ATP and calcium ions, the fibres are able to perform oscillatory work when subjected to small sinusoidal length changes in the frequency range 2-30 c/s.3. When subjected to abrupt changes in length, active fibres show a delayed increase in tension after a stretch and a delayed decrease in tension after release.4. The muscle fibres therefore show the mechanical properties characteristic of glycerinated fibres from asychronous insect flight muscles. Some implications of this finding in relation to the evolution of asynchronous muscles are discussed.


The use of dietary-restricted rat intestine for active transport studies. 1. The effect of dietary restriction (sufficient to produce a loss of about 32% of initial body weight) on intestinal active transport has been studied in the rat by the use of sacs of everted mid-small intestine. Eight D-sugars, four L-sugars and two D-amino acids were employed.2. Dietary restriction enhanced the normally occurring active transport of D-galactose, 3-O-methyl-D-glucose and D-methionine. In addition, sacs of dietary-restricted small intestine were able to concentrate in the serosal fluid D-fucose, D-xylose and D-histidine, which sacs of normal rat intestine could not do. The final (1 hr) serosal/mucosal concentration ratios produced for these actively transported substances were independent of net water movement.3. Sugars which were not concentrated in the serosal fluid of sacs of fully fed or dietary-restricted intestine were D-arabinose, D-fructose, D-glucosamine, D-mannose, L-arabinose, L-fucose, L-sorbose and L-xylose.4. The characteristics of D-fucose and D-xylose active transport suggest that they are transported by the mechanism which actively transports D-glucose. The comparatively low content of D-glucose in dietary-restricted intestine, compared with fully fed intestine, may be part of the explanation for observable active transport of D-fucose and D-xylose by dietary-restricted sacs.5. Thinning of the intestinal wall is believed not to be the cause of the enhanced active transport found during dietary restriction.6. The results show that dietary-restricted rat small intestine may, at times, be more useful than fully fed rat small intestine in the study of intestinal active transport.


Interaction of 28Mg with Ca and K in the smooth muscle of guinea-pig taenia coli. 1. The uptake of (28)Mg, and the total tissue content of Mg, Ca, Na and K have been determined in the smooth muscle of the guinea-pig taenia coli. The Mg content was 6.56 m-mole/kg fresh wt. immediately after dissection, falling slowly to 5.11 after 6 hr immersion in Krebs solution at 37 degrees C.2. The Mg content rose to 15.4 m-mole/kg fresh wt. during immersion in isotonic sucrose containing only MgCl(2). It was independent of the Mg(2+) concentration in this solution, but was depressed when K(+) or Ca(2+) ions were added.3. (28)Mg uptake showed three separate phases, extracellular, intermediate and slow. The size of the extracellular phase was proportional to the Mg(2+) concentration in the solution, but the size of the slow phase was constant. The size of the intermediate phase, exchanging with a half-time of a few minutes, was depressed when K(+) or Ca(2+) ions were added.4. The results are compatible with a competition between Mg in the intermediate phase of tracer exchange, and K(+) or Ca(2+) ions for fixed anionic sites in the tissue.


Excitation of group I activated thalamocortical relay neurones in the cat. 1. Extracellular recordings have been made from 134 group I activated neurones in the ventrobasal thalamic complex. One hundred of the neurones were identified as thalamocortical relay neurones by antidromic activation from the projection areas for group I afferents.2. The discharge evoked by group I volleys showed characteristic fluctuations in latency and number of spikes. The mechanisms underlying these phenomena are discussed.3. Half of the relay neurones were activated from afferents in only one of six dissected forelimb nerves innervating muscle groups at the various forelimb joints. Two-thirds of the relay neurones were activated from at least two adjacent synergistic muscles.4. The pattern of group I convergence in the thalamic neurones is compared with that at the cuneate and cortical levels of the group I pathway to the cerebral cortex. It is suggested that integration of information from synergistic muscles occurs at the thalamic level and that integration of information from muscle groups of more unrelated function occurs at the cortical level.5. A few of the thalamocortical cells (15%) were activated by group II muscle afferents, often in the same nerve as that which provided group I excitation. Weakly linked excitation from cutaneous afferents was observed in 39% of the neurones.6. Stimulation of the group I projection area in the first sensorimotor cortex at moderately high stimulus frequencies produced a trans-synaptic excitation in most of the group I activated cells.


Fat metabolism and heat production in young rabbits. 1. The rates of oxygen consumption were measured in 6-8-day-old rabbits at 34 and 15 degrees C after varying periods of starvation and cold exposure. At the start of the experiment the rabbits had been fasted for 24 hr. Eight rabbits were studied immediately, six after 24 and six after 48 hr in a cold environment (20 degrees C), and twelve after a further 48 hr in a warm environment (34 degrees C). All the animals had a similar increase in oxygen consumption during the final hour of cold exposure (15 degrees C).2. The rabbits kept at 20 degrees C lost 83% of the fat stored in their brown adipose tissue within 24 hr and a further 11% in the next 24 hr. The fat content of white adipose tissue had fallen by 75% at 48 hr. In contrast rabbits kept unfed at 34 degrees C had lost 47% of the fat in brown adipose tissue and 44% of the fat in white adipose tissue after 48 hr.3. In six rabbits subcutaneous thermocouples demonstrated that local heat production continued in brown adipose tissue after 48 hr cold exposure.4. In the rabbits kept at 34 degrees C the final cold exposure caused a large increase in the serum free fatty acid and glycerol concentrations. Much lower concentrations were found in rabbits kept at 20 degrees C.5. The results show that the fat stored in the brown adipose tissue of young rabbits exposed to cold is preferentially used for heat production. When this store of fat is exhausted, brown adipose tissue still produces heat presumably by oxidizing fat and glucose taken from the circulation.


Afferent connexions to group I activated cells in the main cuneate nucleus of the cat. 1. Extracellular recordings were made from a total of 240 group I activated cells in the main cuneate nucleus. Cuneothalamic relay neurones (128) were identified by antidromic stimulation of the medial lemniscus in the ventrobasal thalamic complex.2. A majority of the relay neurones were activated by afferents in only one of six dissected forelimb nerves innervating muscle groups at various joints. Even among afferents from adjacent synergistic muscles, convergence to individual neurones was infrequent.3. Some of the relay neurones received excitation from group II muscle afferents in the same nerve that provided group I excitation. Excitation from group II muscle afferents in other nerves was uncommon. Some neurones were weakly excited by cutaneous volleys.4. Inhibition of group I relay cells was produced from cutaneous afferents and group II muscle afferents. Weak inhibition was sometimes observed from group I afferents. The relay cells were also inhibited by stimulation of the cerebral cortex with a focus around the lateral end of the cruciate sulcus. A good correspondence was found between the inhibition and the depolarization of group I afferent terminals in the cuneate nucleus.5. A majority of the group I activated cells not antidromically activated from the ventrobasal complex (;non-relay cells') were excited by cortical stimulation. Excitation from cutaneous afferents and group II muscle afferents was frequently found among these cells.6. The group I activated cells were found almost exclusively in the ventral part of the nucleus.7. The pattern of convergence found in eleven group I activated cells in the dorsal horn of the spinal cord from C 2 to C 4 is described.


Sweat gland function of the donkey (Equus asinus). 1. Donkeys sweat on exposure to heat and in response to intravenous adrenaline infusion.2. Thermal sweating was abolished by the adrenergic-neurone blocking agent bethanidine but not by atropine.3. Sympathetic decentralization (by preganglionic sympathectomy) abolished thermal sweating but adreno-medullary denervation had no effect.4. Exercise resulted in sweating from both sympathetically innervated and decentralized skin and from the innervated skin of animals which had previously undergone adreno-medullary denervation.5. Insulin-induced hypoglycaemia resulted in sweating from sympathetically decentralized skin and from innervated skin in two out of four animals. Adreno-medullary denervation abolished the sweat gland response to insulin administration.6. Cold exposure inhibited the response of innervated sweat glands but not that of decentralized glands to adrenaline infusion.7. It was concluded that heat-induced and exercise-induced sweating of the donkey is controlled by adrenergic nerves; adreno-medullary secretion may contribute to sweating during exercise, and that cutaneous blood flow is important in the response of the glands to humoral stimulation.


The survival of spermatozoa in bovine cervical mucus and mucus fractions.


Studies of effects of t-alleles in the house mouse on spermatozoa. II. Quasi-sterility caused by different combinations of alleles.


Renal excretion of urea and electrolytes in sheep and red deer. 1. The effects of varying the intake of water and nitrogen on the renal excretion of urea and electrolytes has been studied in sheep and in red deer.2. The concentration of urea in the urine of both sheep and deer decreased as the volume of urine increased and about eight times as much urea was excreted each day when a high-nitrogen diet was fed compared to when a low-nitrogen diet was fed.3. Both the concentrations of sodium and potassium in the urine decreased as the urine volume increased but the total amounts excreted were independent of urine volume.4. The osmotic concentration of the urine decreased as urine volume increased.5. In both deer and sheep the concentration of urea in the plasma was considerably higher when a high-nitrogen diet was fed compared to when a low-nitrogen diet was fed but the rate of clearance of inulin from the plasma did not appear to be related to the diet.6. Appreciably more urea was filtered at the glomerulus when a high-nitrogen diet was fed compared to when a low-nitrogen diet was fed but the percentage of filtered urea reabsorbed was not related to diet.7. The results indicate no qualitative differences between deer and sheep in the renal excretion of urea and electrolytes.


Effects of a hexoestrol derivative, erythro-MEA, on early pregnancy in the mouse.


Incorporation of labelled nucleosides in vitro by rabbit and mink blastocysts in the presence of blastokinin or serum.


Effects of hexamethylphosphoramide on rat spermatogenesis and fertility.


Effect of progesterone on induced ovulation in immature rats.


Inhibition of HCG-induced ovulation by anti-HCG serum in immature mice pre-treated with PMSG.


Ageing of rabbit spermatozoa in the male tract and its effect on fertility.


Motion picture analysis of ejaculation in the bull.


Morphological responses to pituitary gonadotrophins by mouse ovaries in vitro.


Effect of a single dose of human chorionic gonadotrophin on the ovulatory response of the immature rat.


Relationship of a trypsin-like enzyme in rabbit spermatozoa to capacitation.


The adopted child as a fertility charm.

The adopted child as a fertility charm.


Reproductive behaviour in the sheep and pig after transplantation of the ovary.


Cardiac glycoside binding and alkali cation transport in mature and epididymal bull spermatozoa.


Big and little strokes.

Big and little strokes.


"G" is for general and geriatric.

"G" is for general and geriatric.


High blood pressure and low blood pressure in the elderly.


Medical aspects of geriatric rehabilitation.


Nursing aspects of geriatric rehabilitation.


Physiotherapy in geriatric rehabilitation.


Occupational therapy in geriatrics.

Occupational therapy in geriatrics.


Social problems in geriatric rehabilitation.


The range of local authority services in this country.


The rôle of the district nurse.

The rôle of the district nurse.


Domestic help service.

Domestic help service.


Mental aspects of cerebrovascular disease.


A laser manipulator for surgical use.

A laser manipulator for surgical use.


Determination of fumigant residues in cereals and other foodstuffs: a multi-detection scheme for gas chromatography of solvent extracts.


A straight needle-probe for the measurement of blood velocity.


Split renal function studies versus renal vein renins: a comparison in diagnosis and treatment in renal vascular hypertension.


The distribution of tetracycline in renal tissue during pyelonephritis.


Torulopsis glabrata pyelonephritis.

Torulopsis glabrata pyelonephritis.


A case of epidermoid carcinoma of the larynx with metastases to the kidney.


Use of inferior venacavography in the evaluation of renal neoplasms.


Experimental renal arteriovenous fistula.


Audio amplification in pyelolithotomy and nephrolithotomy.


Inclusion cyst of the renal pelvis: case report.


Unusual renal peripelvic extravasation requiring operative drainage.


A new method to create a submucosal ureteral tunnel.


Urethrotomy visualized for bladder neck contracture in male patients.


Hemangioma of the bladder: report of a case and review of the literature.


Distant metastases from beta-naphthylamine induced vesical tumors in dogs.


Erythroplasia of Queyrat treated with topical 5-fluorouracil.


Ectopic spleen simulating testicular tumor.


Bone marrow biopsy in patients with carcinoma of the prostate.


Prevention of estrogen-induced gynecomastia by external irradiation.


An improved ureteroileostomy appliance.

An improved ureteroileostomy appliance.


Air pyelogram in a case of undiagnosed unilateral urinary bleeding.


A new 3-way Foley catheter.

A new 3-way Foley catheter.


Irrigating water bottle holding device for cystoscopy rooms.


A modified perineal retractor.

A modified perineal retractor.


Pyeloureterostomy and ureteroureterostomy: alternative procedures to partial nephrectomy for duplication of the ureter with only one pathological segment.


Neonatal ascites: perirenal urinary extravasation with bladder outlet obstruction.


Perinephric pancreatic pseudocyst in a child.


Reduplication of the bladder by incomplete frontal septum and associated anomalies.


Testicular infarction due to strangulated inguinal hernias in infants.


Juvenile dystonic lipidosis.

Juvenile dystonic lipidosis.


Effect of amiloride hydrochloride (MK-870) in patients with cirrhosis.


Perforated appendicitis in premature infants. A case report and review of the literature.


Dolicho-ectatic intracranial arteries: symptomatology and pathogenesis of arterial elongation and distention.


Cytotoxic effects of a nitrofuran derivative on an ascites carcinoma cells.


Clinical studies of interferon. II. Recovery of interferon from cerebrospinal fluid of patients with aseptic meningitis.


Studies on lipogenesis in hereditary obese-hyperglycemic mice (KK strain).


Glycolytic and gluconeogenic metabolites and enzymes in the liver of obese-hyperglycemic mice (KK) and alloxan diabetic mice.


A new method for serum lipase determination and serum lipase activity in liver diseases determined by this method.


Studies on the variation of serum sialic acid and hexosamine contents in the Sprague-Dawley rats with the experimental bone tumor.


Renal angiography in urology.

Renal angiography in urology.


Effect of insulin on mitochondrial respiratory control and oxidative phosphorylation of alloxan diabetic rats. A possible new action of insulin on liver mitochondria.


Protein binding and vascular activity of diazoxide.


Bronchogenic cysts of the mediastinum in infancy.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 47-1969.


Intensive coronary care--a practical system for a small hospital without house staff.


Genesis of hepatitis.

Genesis of hepatitis.


Drugs, receptors and serum protein binding.


Use of BCG is defeatist.

Use of BCG is defeatist.


Hazards of electricity.

Hazards of electricity.


Platelet transfusion therapy; the selection of compatible platelet donors for refractory patients by lymphocyte HL-A typing.


Radiographic identification of commonly used implanted pacemakers.


Smallpox vaccination.

Smallpox vaccination.


Assay of digitalis concentrations in blood.


Tay-sachs disease.

Tay-sachs disease.


Study of human reproduction.

Study of human reproduction.


Bleeding after cardiac catheterization.

Bleeding after cardiac catheterization.


When to treat leukemia.

When to treat leukemia.


EACA and intravascular coagulation.

EACA and intravascular coagulation.


Binding by plasma globulins.

Binding by plasma globulins.


Localization of parathyroid adenoma.

Localization of parathyroid adenoma.


Rehabilitation of patients with strokes.


Current concepts of fat embolism.

Current concepts of fat embolism.


Gastric outlet obstruction following alkali ingestion.


Chronic intravascular coagulation and microangiopathic hemolytic anemia in a patient with carcinoma of the lung.


Findlay physician plays leading role in MARCO, worldwide information exchange via "ham" radio.


The use of l-dopa in parkinsonism.

The use of l-dopa in parkinsonism.


Responding to the challenge.

Responding to the challenge.


Pediatric manpower needs: can they be met?


The crisis in medical care.

The crisis in medical care.


Not in our own image: educating the pediatrician for practice.


The medical center and the continuing education of the practicing physician.


Professor-practitioner exchange program.


The values of a personal pediatrician.

The values of a personal pediatrician.


Differential charging in pediatric practice.


The Kaiser-Permanente Plan in child health care.


Experience with prepayment financing in a private pediatric practice.


The federal government in child health care.


Third-party payment of medical benefits.


Some essentials in a national program for child health: a summary.


Basic principles in manpower utilization.


Allied health personnel in child health care.


A practicing pediatrician looks at associates, assistants, and aides.


The case for solo practice.

The case for solo practice.


The place of the general practitioner in child health care.


Preventive services for children.

Preventive services for children.


The role of the American Academy of Pediatrics in child health care.


Peiatric subspecialty group practice.

Peiatric subspecialty group practice.


Service and availability: essential components of better child health care.


Is your office a land of giants?

Is your office a land of giants?


The drive-in pediatrics office.

The drive-in pediatrics office.


Viruses in fungi.

Viruses in fungi.


Genetic counselling.

Genetic counselling.


Heterologous (Chimera) cross circulation for hepatic failure.


Serum transaminase levels after closed mitral valvotomy.


Acute suppurative adenitis in children: a review of 964 cases.


Limb gangrene associated with rheumatoid arthritis. Clinico-pathological conference.


Occult rupture of the spleen.

Occult rupture of the spleen.


Antibiotic prevention of experimental hemorrhagic pancreatitis in germ-free and conventional dogs.


Excision of malignant carotid body tumor: resection of carotid bifurcation with autogenous vein graft.


Electrophysiological tracings of intraretinal optic nerve fibers in the cat.


Hypocupremic infant associated with mental retardation and hepatic cirrhosis: probably a copper malabsorption syndrome.


Effects of Mn and Zn ions on the contraction of smooth muscle of guinea-pig Taenia coli.


Effects of Mn, Zn and Mg ions on the electrical activities of smooth muscle of guinea-pig taenia coli.


A further investigation on the nature of sweat discharge in man.


Clinical trials of a new antitumor polypeptide: neocarzinostatin (NCS).


Effects of iron deficiency on blood level of sulfhydryl compounds and on structure of gastric mucosa in rats.


Some normal values for blood and organs in the golden hamsters.


The organophosphorus warble fly dressings: some aspects of their toxicity to cattle including antidote therapy.


DNA content of Mauthner cell nuclei in Xenopus laevis: a spectrophotometric study.


Differentiation of the hypothalamic nuclei during ontogenetic development in the rat.


Physical activity of children with congenital heart disease.


The polarizing treatment for myocardial infarction.


Symposium on the pre-hospital phase of acute myocardial infarction. I. Introduction.


Problems of the pre-hospital phase of acute myocardial infarction.


Sudden death in arteriosclerotic heart disease; the case for preventive medicine.


The frequency of asymptomatic disturbances of cardiac rhythm and conduction in middle-aged men.


Factors contributing to delay in responding to the signs and symptoms of acute myocardial infarction.


Delay in hospitalization during the acute coronary period.


Pre-hospital coronary care. The mobile coronary care unit.


Models for the evaluation of pre-hospital coronary care.


The significance of atrial rate in patients with atrioventricular conduction abnormalities complicating acute myocardial infarction.


Effects of glucose and nonglucose infusions on myocardial potassium ion transfers and arrhythmias during ischemia.


Dextrothyroxine for lowering serum cholesterol. Analysis of data on 6066 patients.


Bilateral subcapsular hematoma after needle biopsy of one kidney.


The extrapleural ligation of patient ductus arteriosus in the seriously ill infant.


Meanders of the great saphenous vein.

Meanders of the great saphenous vein.


The meandering phenomenon in the pathogenesis of varices.


The morphology of early meanders of the great saphenous vein.


Limb volume and blood flow changes during the menstrual cycle. I. Limb volume changes during the menstrual cycle.


Atrial standstill: a review, and presentation of two new cases of familial and unususal nature with reference to epicardial pacing in one.


Limb volume and blood flow changes during the menstrual cycle. II. Changes in blood flow and venous distensibility during the menstrual cycle.


Diaphragm transplantation; a preliminary report on an experimental form of permanent myocardial assistance.


The influence of detergents on the skin (a clinical and biochemical study).


Metabolic control mechanisms in mammalian systems. 8. Estrogenic induction of fructose-1,6-diphosphate aldolase in the rat uterus.


The inhibition of aspartate transcarbamolylase activity by ATP in a mutant of baker's yeast S1237.


Uptake and deamination of adenosine by blood. Species differences, effect of pH, ions, temperature and metabolic inhibitors.


Identification of epsilon-N-methyl-L-lysine and delta-N-methylornithine in bovine brain.


The biosynthesis of salicylic acid in Mycobacterium smegmatis via the shikimic acid pathway.


A comparative study of the action of release inducers upon platelet release and phosphorus metabolism.


Hyaluronate-peptide linkage group.

Hyaluronate-peptide linkage group.


Glutathione. 3. Biological aspects of the azoester procedure for oxidation within the normal human erythrocyte.


Glutathione. I. The methyl phenyldiazenecarboxylate (azoester) procedure for intracellular oxidation.


Glutathione. IV. Intracellular oxidation and membrane injury.


Enzymic and nonenzymic transformations of chorismic acid and related cyclohexadienes.


Enzymatic synthesis of O-alkylhomoserine.


Sequential steps in the synthesis of hydroxylysine and the glycosylation of hydroxylysine during the biosynthesis of collagen.


Glutathione. II. Chemical aspects of azoester procedure for oxidation to disulfide.


Development of protocollagen proline hydroxylase activity in the chick embryo.


Separation of kinin-forming factors in human plasma.


Experimental wound sepsis from transient Klebsiella pneumoniae bacteraemia.


An investigation of a possible humoral factor produced by the thymus in terms of its effect on immunological competence.


Oxygen consumption of ischaemic rat brain in vitro.


Wound contraction in experimental lathyrism.


The anterior tibial compartment syndrome in a twenty month old infant. (A complication of the use of a bow leg brace).


An oestrogen-linked nephrocalcinosis in rats.


The functional attitude of the hand afflicted with an anterior interosseous nerve paralysis.


Carpal tunnel syndrome caused by calcification in the deep or volar radio-carpal ligament.


Extruded lumbar intervertebral discs in pseudospondylolisthesis.


Unicameral bone cyst, 15 year follow-up.


A simple method of tibial insertion of a torn internal collateral ligament of the knee.


The extensor mechanism of the fingers.

The extensor mechanism of the fingers.


Muscular and tendinous variations of the flexor superficialis of the fifth finger of the hand.


Scoliosis starting years after cessation of growth.


The value of laminectomy in benign and malignant extradural spinal cord compression.


Acrylic granuloma of the radius after a lucite prosthesis to replace the broken scaphoid bone. Follow-up of sixteen years.


Resection of metatarsal heads for metatarsalgia.


Adult human enamel. I. Influence of grinding upon X-ray diffraction profile breadth.


Skeletal uptake of simultaneously ingested fluoride and calcium in the rat.


The effect of some inhibitors on the nucleation and crystal growth of apatite.


Calcium carbonate (calcite) as a separate phase besides calcium phosphate apatite in medullary bone of laying hens.


Mitochondria, calcification and waste disposal.


The management of diabetic coma.

The management of diabetic coma.


Rubella. Technical problems in the performance of hemagglutination-inhibition (HI) tests. The rubella hemagglutination inhibition (hi) test is the most sensitive serologic method available for diagnosis of infection and for determination of immunity status. At present, however, it is not a well-standardized procedure and several modifications are in use. Comparison of results obtained on the same specimens by different laboratories has shown wide variations. The sensitivity and reliability of the technique is decidedly influenced by each of the variables of the test system; these include erythrocytes, antigen, methods employed for removal of non-specific inhibitors and natural agglutinins from test sera, the pH of the diluents and the temperature of incubation. The presence or absence of hi antibody cannot be determined reliably by screening a single low dilution of serum. Commercially available reagents and kits for rubella hi tests vary widely in their reliability. The test should be performed only by experienced persons who understand the principles of the technique and who are aware of the pitfalls and of the importance of using adequate controls in each run.


Benign micturition syncope.

Benign micturition syncope.


Porphyria cutanea tarda remission by venesection.


Congenital adrenal cortical hyperplasia--successful long-term treatment.


Chemotherapy guide.

Chemotherapy guide.


Sulfonamides.

Sulfonamides.


Antibiotics and antibacterials, general.


Drugs of choice for specific bacteria.

Drugs of choice for specific bacteria.


Investigational antimicrobial agents.

Investigational antimicrobial agents.


Antibacterial agents in renal insufficiency.


Eosinophilia and eosinophilic carditis.

Eosinophilia and eosinophilic carditis. These discussions are selected from the weekly staff conferences in the Department of Medicine, University of California Medical Center, San Francisco. Taken from transcriptions, they are prepared by Drs. Martin J. Cline and Hibbard E. Williams, Associate Professors of Medicine, under the direction of Dr. Lloyd H. Smith, Jr., Professor of Medicine and Chairman of the Department of Medicine.


Antibiotics and liver disease.

Antibiotics and liver disease.


A retraining program for inactive physicians. During the past two years a pilot project was conducted in which 19 inactive physicians were retrained in preparation for resumption of active practice. The initial program consisted of a flexible training program of six months to one year patterned after conventional internship-residency concepts. During the second year the program was modified by providing an initial condensed indoctrination period of two months' duration especially designed for this purpose, followed by a preceptorship type of training. The project was considered successful in permitting trainees to enter some form of active medical work, or to enroll in formal specialty training. The observations made by the faculty of the program and its accomplishments are discussed in the light of the effort expended and the cost of the project.


Professional liability and patient care.


Management of diabetic coma.

Management of diabetic coma.


Phlebotomy treatment of porphyria cutanea tarda: is it really effective?


Cystic fibrosis in children and adolescents.


The physician, his association and health care.


Mumps virus vaccine.

Mumps virus vaccine.


Prosthetic replacement of the aortic valve: a current assessment of operative results.


Symposium on world plant nutrient reserves and their conservation.


World potash resources.

World potash resources.


Water resources and plant nutrient reserves.


Will the world demand for nitrogen be met?


Chemicals used in agriculture and food storage.


Sulfur: a new abundance.

Sulfur: a new abundance.


Fatty acids. 20. The base-catalysed rearrangement of vernolic and other epoxy esters: the partial synthesis of methyl coriolate, methyl dimorphecolate, and some conjugated polyenoic esters by a possible biosynthetic route.


Fatty acids. 21. The rearrangement of methyl 12, 13-epoxyoleate by boron trifluoride with formation of cyclopropane esters.


Studies on the lysis of red cells and bimolecular lipid leaflets by synthetic lysolecithins, lecithins and structural analogs.


Gas chromatography--mass spectrometry of O-methyloxime derivatives of prostaglandins.


The modified synthesis of phosphatidyl ethanolamine.


The condensation of aziridine with phosphatidic acid; synthesis of O-(1,2-diacyl-sn-glycero-3-phosphoryl)-ethanolamine.


The reaction of glutaraldehyde with tissue lipids.


The structure of the main urinary metabolite of prostaglandin F2-alpha in the guinea pig.


Substrate activation with a xanthine oxidase reaction. An alternative to dismutation as an explanation in the chicken liver system.


Early malnutrition and human development.


Enzymatic oxidation of bilirubin.

Enzymatic oxidation of bilirubin.


Adenine nucleotide translocation of mitochondria. Kinetics of the adenine nucleotide exchange.


Separation of N-acetyl-alpha-glucosaminidase and N-acetyl-alpha-galactosaminidase from ox spleen. Cleavage of the O-glycosidic linkage between carbohydrate and polypeptide in ovine and bovine submaxillary glycoprotein by N-acetyl-alpha-galactosaminidase.


Adenine nucleotide translocation in mitochondria. Quantitative evaluation of the correlation between the phosphorylation of endogenous and exogenous ADP in mitochondria.


Fractionation and characterization of glycoproteins containing hydroxyproline from the leaves of Vicia faba.


Mechanism of peroxide-inactivation of the sulphydryl enzyme glyceraldehyde-3-phosphate dehydrogenase.


Chemoprophylaxis in tuberculosis control.


A case of visceral leishmaniasis or kala-azar in the Netherlands.


A disposable nylon-mesh-supported cuprophane envelope for the Kiil haemodialyzer: studies of the in vitro performance.


Massive ascites in portocaval anastomosis.


"Low" versus "high" concentration chymotrypsin in gastric exfoliative cytology.


Effect of pH on intrinsic factor and nonintrinsic factor vitamin B12 binding in human gastric juice neutralized in vivo.


Gastric secretion in the chronic fistula ferret.


Importance of bile acids for phospholipid secretion into human hepatic bile.


Evaluation of portal circulation in hepatic cirrhosis. A new method using xenon.


Small intestinal mucosa in cholesterol ester storage disease. A light and electron microscope study.


Poorly absorbed sulfonamides in the treatment of tropical sprue.


Intestinal antisepsis. Stability of fecal flora during mechanical cleansing.


Congenital stenosis of the first part of the duodenum in an adolescent.


Mesenteric vascular diseases.

Mesenteric vascular diseases.


Central nervous control of gastric secretion in health and in duodenal ulcer.


On intrinsic factor and the dog.

On intrinsic factor and the dog.


The concept of potentiation.

The concept of potentiation.


The small intestine in human schistosomiasis.


Source of electron energy for animal metabolism. I. Role of ascorbic acid in the metabolism of avian muscle.


Some aspects of the Feulgen reaction in situ.


Sialic acids in the salivary glands of some insects with different feeding habits.


Substrate structure effects on the pH dependence of deacylation of acyl-chymotrypsins.


Uptake and accumulation in vitro of 3H-noradrenaline in adrenergic nerves of human atrium.


Ultrastructural localization of glutamic oxalacetic transaminase activity in cardiac muscle fiber and cardiac mitochondrial fraction of the rat.


Synthesis of the fully protected, carboxyl-terminal tetradecapeptide sequence of staphylococcal nuclease.


A cyclobutanol intermediate in the decomposition of aldehydes under electron impact. A further photochemical analogy.


Larval nematodes from fish of the subfamily Anisakinae and gastro-intestinal lesions in mammals.


The histopathology and pathogenesis of demodecosis of the dog.


Aetiology of "yellow fat" disease (pansteatitis) in the wild rabbit.


Susceptibility of chickens, mice and chick embryos to sindbis, Murray Valley encephalitis and Getah viruses.


Failure of effective insemination of young female Aedes aegypti mosquitoes.


Movement of potassium and sodium across the midgut epithelium of the American cockroach.


Programmed cell death. Activation of lysis by a mechanism involving the synthesis of protein.


An ultrastructural analysis of microsomal preparations from Musca domestica and Prodenia eridania.


The utilization of amino acids and sugars by tick cells cultivated in vitro.


Brain interneurons in noctuid moths: differential suppression by high sound intensities.


The effect of sodium replacement on the electroretinogram recorded from the cockroach ocellus.


Free amino acid composition of the ageing female mosquito Aedes aegypti as determined by automatic ion-exchange chromatography.


Ahmad-Strong synthesis of 8-, 9-, and 10-pentadecynoic acids. Three pentadecynoic acids, with the triple bond in the 8-, 9-, and 10-positions, have been synthesized on a gram scale in over-all yield of 65% by refinements of the five-step Ahmad-Strong method; isolation of intermediates was shown to be unnecessary prior to purification of the acetylenic nitriles by column chromatography on silicic acid. The melting points of the pentadecynoic acids alternate regularly and widely with position of unsaturation, in marked contrast to behavior of the homologous octadecynoic acids described by Huber.


Biosynthesis of galactolipids by enzyme preparations from spinach leaves. The pH optimum for galactolipid synthesis from UDP-galactose by spinach chloroplasts is 7.2 in Tris-HCl or phosphate buffer. The products include sterol glycosides, trigalactosyl diglyceride (tentatively identified), digalactosyl diglyceride, and monogalactosyl diglyceride in increasing order of quantity. The proportion of monogalactosyl diglyceride decreases and that of digalactosyl diglyceride increases as the pH is lowered. The galactolipid synthesis is quite resistant to elevated temperature; maximal incorporation of galactose from UDP-galactose was observed at 45 degrees C. The proportion of monogalactosyl diglyceride was greater at the higher temperatures. As much as 40% of the galactolipid-synthesizing capability of a spinach leaf homogenate is not sedimented by centrifugation for 60 min at 100,000 g. An acetone powder of spinach chloroplasts contains enzymes which catalyze galactolipid synthesis. This preparation is dependent on added diglycerides in order to make galactolipid, whereas the chloroplast preparation is not dependent on added diglycerides. Molecular species of diglycerides were compared as requirements for galactolipid synthesis. The requirement was satisfied best by the diglycerides of highest unsaturation. Methylation of the free hydroxyl of the diglyceride eliminated the effectiveness.


Tritioboration and synthesis of tritium-labeled polyunsaturated fatty acids. Methyl esters of polyunsaturated fatty acids labeled with tritium were prepared by partial stereoselective reduction of the corresponding acetylenic esters with tritiated disiamylborane, followed by protonolysis with tritiated acetic acid. The labeling was strictly specific, and the tritium atoms were located only at the carbon atoms forming the unsaturated bond(s). Synthesis of some tritiated fatty acid methyl esters with methylene-interrupted trans-cis double bonds is also reported.


Effect of the stress of unilateral adrenalectomy on the depletion of individual cholesteryl esters in the rat adrenal. Normal rats were subjected to unilateral adrenalectomy and were killed 3 hr later. The concentration and composition of the cholesteryl esters in adrenals removed at operation and after death were compared. The esterified cholesterol concentration was lower in the adrenals obtained 3 hr after surgery. Cholesteryl arachidonate decreased in concentration significantly more than any other ester, followed in order of magnitude by linoleate and oleate. The cholesteryl ester concentration of adrenals removed from sham-operated rats 3 hr after surgery was also greatly reduced. On the basis of comparison with other work on the hydrolysis of cholesteryl esters by adrenal homogenates, it is concluded that the apparent selectivity in depletion of cholesteryl esters is due to differences in their rates of hydrolysis.


Feedback regulation of bile acid biosynthesis in the rat. The hepatic biosynthesis of bile salts in the rat has been shown to be controlled homeostatically by the quantity of bile salt returning to the liver via the portal circulation. The feedback mechanism was demonstrated in two kinds of experiments. In the first, rats with bile fistulas were infused intraduodenally with sodium taurocholate 12 hr after surgery. If the rate of infusion was greater than 10 mg per 100 g rat per hr, the increase in bile acid output normally observed in bile fistula rats was prevented. In the second type of experiment, the rats were infused with taurocholate 48-72 hr after biliary diversion, when bile acid output had reached a maximal value. Provided the rate of infusion exceeded 10 mg per 100 g rat per hr, bile acid secretion returned to the low levels observed in intact rats. Previous attempts to demonstrate the feedback control have been unsuccessful because too little bile salt was infused. The taurocholate pool of the experimental animals was measured as approximately 15 mg per 100 g rat; it was calculated from this and the above results that this pool circulated 10-13 times daily.


Improved quantitation of plasma lipids by direct gas-liquid chromatography. A preliminary digestion of total plasma lipid extracts with phospholipase C, which converts the lysolecithins, lecithins, and sphingomyelins into monoglycerides, diglycerides, and ceramides, respectively, has been shown to facilitate subsequent determination of the plasma lipids by gas-liquid chromatography. A further improvement in the chromatographic elution pattern results from acetylation or trimethylsilylation of the liberated alcohol moieties prior to injection into the chromatograph. If tridecanoin is used as internal standard, quantitative estimates can be rapidly obtained for plasma lysolecithins, free cholesterol, lecithins, sphingomyelins, cholesteryl esters, and triglycerides, as well as for free fatty acids. Other plasma lipids do not occur in sufficiently high concentrations to interfere with the analysis. The determination requires 0.1-0.5 ml of plasma and about 6 hr of processing, but many samples can be processed at a time.


Hepatic acetoacetyl-CoA deacylase activity in rats fed ethyl chlorophenoxyisobutyrate (CPIB). Intact or sonicated mitochondria from the livers of rats fed a diet containing 0.2% ethyl chlorophenoxyisobutyrate (CPIB) for 3 wk showed acetoacetyl-CoA deacylase activity enhanced 26 and 39%, respectively, over that shown by comparable fractions from rats fed the same diet without CPIB. The corresponding supernatant fractions did not differ in activity. The enhanced activity of mitochondrial acetoacetyl-CoA deacylase in the livers of the CPIB-treated rats could effectively decrease the amount of acetoacetyl CoA available within the cell for synthetic processes.


Dietary protein and the control of fatty acid synthesis in rat adipose tissue. Fatty acid synthesis in adipose tissue normally proceeds at a high rate when fasted animals are refed a diet containing carbohydrate, protein, and low levels of fat. This study investigated the effect of omitting protein from the refeeding diet. Rats were fasted for 48 hr and refed either a protein-free diet or a balanced diet, and the rate of fatty acid synthesis from glucose, pyruvate, lactate, and aspartate was measured. Refeeding the animals a diet devoid of protein resulted in a low rate of fatty acid synthesis from each of these substrates as well as a reduction in carbon flow over the citrate cleavage pathway. The activities of glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, NADP-malate dehydrogenase, and ATP-citrate lyase were also reduced in epididymal fat pads from these rats. On the other hand, adipose tissue phosphoenolpyruvate carboxykinase activity was five times as great as that in tissue from animals refed a balanced diet. This difference could be eliminated if actinomycin D was injected coincident with refeeding. Refeeding rats diets high in carbohydrate is not, therefore, capable of inducing high rates of fatty acid synthesis in adipose tissue in the absence of dietary proteins. Thus, liver and adipose tissue respond differently to dietary protein.


Improved synthesis of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). An improved method for the chemical synthesis of HMG-CoA is described. The procedure is designed to prevent the formation of 3-acetoxy-HMG-CoA.


Mass spectrometric analysis of long-chain esters of diols. Homologous series of synthetic long-chain monoesters and diesters of 1,2-ethanediol were analyzed by mass spectrometry, and the patterns of fragmentation were studied. Under electron impact saturated ethanediol monoesters yielded prominent ions characteristic of the short-chain diol, such as the rearranged ion formed by 2,3-cleavage (m/e 104) and the ion caused by 3,4-cleavage (m/e 117). Fragments characteristic of the constituent long-chain moieties were the acylium ions [RCO](+), [RCO - 1](+), and the ions [RC(OH)(2)](+). The mass spectra of ethanediol diesters exhibited very intense peaks due to the ions formed by loss of the acyloxy group, [M - RCOO](+), or one carboxylic acid, [M - RCOOH](+). These ions are distinctive for diol diesters. Precise mass measurements by high resolution mass spectrometry verified the composition of the ion fragments. Spectral studies of some monoesters and diesters of 1,3-propanediol, 1,4-butanediol, 2,3-butanediol, and also of some monounsaturated homologues, demonstrated that mass spectrometry is very suitable for the identification, distinction, and analysis of diol lipids.


A synthesis of methyl 2,3,6-trideoxy-alpha-D-erythro-hexopyranoside (methyl alpha-amicetoside).


Binding of phosphatidylcholine-14C to glass. Lecithin-(14)C was found to bind to Pyrex glass. The percentage bound varied with different organic solvents, being greatest in dioxane solution. Thorough treatment of the glass with dimethyldichlorosilane prevents the binding, which suggests that charge-charge interactions are involved.


A novel thiazole synthesis. 4,5,6,7-tetrahydrothiazolo[4,5-d]pyrimidine-5,7-diones.


Observation of a large isotope effect in the manganese dioxide oxidation of benzyl alcohol.


Liquid crystals. II. Cholesteric properties of some 9,19-cyclopropane triterpene fatty acid esters.


Aminoacyl nucleosides. IV. The rearrangement of N6-(alpha-aminoacyl)adenines to N-(6-purinyl)amino acids. Application to adenosine analogs.


Aminoacyl nucleosides. V. The mechanism of the rearrangement of N6-(alpha-aminoacyl)adenines into N-(6-purinyl)amino acids.


The synthesis of 17-deoxy-17-alpha and 17beta 20-pregnynes and -20-pregnenes.


Preparation and properties of steroidal 17,20- and 20,21-acetonides epimeric at C-20. I. Derivatives of 5beta-pregnan-3alpha-o1.


Preparation and properties of steroidal 17,20- and 20,21-acetonides epimeric at C-20. II. Derivatives of cortisol and cortisone.


Anomeric C-19-steroid N-acetylglucosaminides.


Synthetic studies related to the yohimbine alkaloids.


A new synthesis of selenoureas and selenothiocarbamic esters from thioureas.


Linear free-energy relationship between alcohol pKa and solvolysis rates of esters where substituent variation is in the alkyl portion of the ester.


Heterocyclic ring-closure reactions. 3. 5-aminoimidazoles from azomethines.


Irradiation effects on the bound water of mammalian tissues.


Alkylation by alcohols in the prescence of dicyclohexylcarbodiimide. Alkylation of thymine, uracil, thymidine, and uridine by alcohols.


On the simultaneous measurements of the particle size. Distribution of radioactive charged aerosols and concentration ratio of positive to negative (the second report).


Total ground deposition of strontium-90 in Japan (1).


Abolition of the crabtree effect in Ehrlich ascites tumor cells by gamma-irradiation.


Gamma ray spectrometry of the natural radioactivity in the airborne dust.


32P-retention of DNA irradiated in a reactor. The enhancement of the retention with increasing irradiation time.


The effect of prolonged breast-feeding on radiocesium retention in young rat.


The effect of potassium, lactose and thyroxine administration on radiocesium retention in young rat.


Experimental evaluation of myocardial tunnelization as a method of myocardial revascularization.


The hemodynamics and coronary arteriography patterns during acute myocardial infarction.


The effect of excesively high pefusion pressures on the histology, histochemistry, birefringence, and function of the myocardium.


Postoperative ventricular arrhythmias caused by isoproterenol. Conversion with insulin.


An operative technique for the control of reflux following esophagogastrostomy.


The expeimental treatment of esophageal strictures by intralesional steroid injections.


Improved organ function during cardiac bypass with a roller pump modified to deliver pulsatile flow.


Relation of pulsatile flow to oxygen consumption and other variables during cardiopulmonary bypass.


A central-flow, low-profile, leaflet-deforming mitral valve prosthesis free of prolonged anticoagulation requirements.


Surgery for severe rheumatic mitral regurgitation in children.


Coarctation of the aorta. The abdominal pain syndrome and paradoxical hypertension.


Pseudocoarctation of the aorta. A reappraisal.


The oxygen tension of cerebrospinal fluid during cardiopulmonary bypass.


A simplified method for management of malignant pleural effusion.


Pancoast's syndrome due to hydatid cysts of the thoracic outlet.


A gastropleural fistula 12 years following gastric interposition for carcinoma of the esophagus.


Studies on the fate of fentanyl.

Studies on the fate of fentanyl.


Effects of xylitol on carbohydrate metabolism in rat liver treated with carbon tetrachloride or alloxan.


Snakebite.

Snakebite.


Genetics and the Natal Indian diabetic.

Genetics and the Natal Indian diabetic.


Ageing, involution, and senescence.

Ageing, involution, and senescence.


Serum zinc and copper concentrations in children with protein-calorie malnutrition.


Teat holes.

Teat holes.


The treatment of snakebite.

The treatment of snakebite.


Injuries to the distal tibial epiphysis.


Stromal endometriosis.

Stromal endometriosis.


Cleft lip palate in Cape Town.

Cleft lip palate in Cape Town.


Ambulance services.

Ambulance services.


The Red Cross air-mercy service.

The Red Cross air-mercy service.


Effect of atropine on platelet structure and function.


Hereditary thrombocytopenia with excessively prolonged bleeding time.


Glycogen in human peripheral blood leukocytes. 3. Effect of female hormones.


Neutrophil segmentation and radial segmentation. Effects of cooling and demecolcine on the lobulation on neutrophil nuclei.


Metformin and fibrinolysis.

Metformin and fibrinolysis.


Permanent fixation of lymphoid cells exposed to dye exclusion test.


Cystine: compartmentalization within lysosomes in cystinotic leukocytes. The large amount of cystine compartmentalized in cystinotic leukocytes cosediments in isopycnic sucrose density gradients with dense lysosomal particles, within which it is presumably contained. Such cystine appears to be primarily noncrystalline in these organelles.


Homology of lactate dehydrogenase genes: E gene function in the teleost nervous system. Data obtained from immunochemical studies and the use of allelic variants support the hypothesis that the lactate dehydrogenase isozymes unique to the teleost nervous system are encoded in a third locus. Immunochemical and other studies demonstrate that this third locus is closely related to the lactate dehydrogenase B locus.


Naturally occurring diol lipids: dialkoxypentanes in porpoise (Phocoena phocoena) jaw oil. Dialkyl ethers of diols (dialkoxyalkanes), naturally occurring lipids, have been isolated from the jaw oil of the porpoise Phocoena phocoena. The principal constituents are dialkoxypentanes containing two 18-carbon chains. The alkoxy linkage may play an important role in the metabolism of the diol lipids.


Oxytocin: effects of degradation on radioimmunologic and biologic activity. Incubation with thioglycollate destroyed biologic activity of oxytocin, but left immunologic activity intact. Incubation with plasma of pregnant women at term or with placental extract destroyed biologic and immunologic activities. Dissociation of biologic from immunologic sites is suggested.