Vagotomy and gallbladder function.

Vagotomy and gallbladder function. The effect of vagotomy on gallbladder function was investigated in a clinical and experimental study. In the clinical study both the size of the gallbladder and its capacity to respond to cholecystokinin were evaluated radiologically before and after vagotomy. In studies in the rabbit, both the immediate effect of vagotomy on the gallbladder and the effect of varying doses of cholecystokinin on gallbladder pressure were studied before and after vagotomy. In studies in the cat the long-term effect of vagotomy was studied with respect to the histology of the gallbladder and the composition of bile. The clinical investigation showed that vagotomy was followed by a significant increase in the volume of the gallbladder and that the effect of the cholecystokinin on the gallbladder remained unchanged after vagotomy. In experiments in the rabbit it was found that cholecystokinin in a dose of 1 unit/kg body weight exerted a somewhat lesser effect on gallbladder pressure after vagotomy than before, while after vagotomy a dose, approximately four times greater, resulted in a stronger gallbladder response. Further, the experiments showed that the chemical composition of the bile seemed to be altered after vagotomy, while the gallbladder remained histologically essentially unchanged.


Ultrastructural study of two cases of alpha-chain disease. Intestinal biopsies of two patients with alpha heavy-chain disease were examined by electron microscopy. Except for a few differences, cells involved by the malignant neoplasia of the small intestine were similar and belonged to the plasma cell line and were, in fact, more like the cells observed in typical multiple myeloma. It is not surprising to find such cells in a disease which is characterized by the presence of alpha heavy chain in the serum since plasma cells are considered to be the main source of immunoglubulins. Moreover, the association of an intestinal plasmacytic neoplasia with the heavy chain of the immunoglobulin A in the serum agree with what is known of the normal synthesis by most of the plasma cells of the digestive tract of this immunoglobulin. Since lymphocytes can synthesize immunoglobulins and plasma cells probably originate from lymphocytes, too great an importance should not be attributed to morphological classification. Only an obvious, functional characteristic seems really useful for differentiation.


Morbidity pattern of out-patient attendance.


Prevention of deleterious oral habits.

Prevention of deleterious oral habits.


Acoustic neurinomas in children.

Acoustic neurinomas in children.


Cornelia de lange syndrome. Report of 2 cases in siblings.


Hypertrophied verumontanum producing urinary obstruction.


Familial leukemia. Report of two cases in siblings.


Drug-induced lupus erythematosus.

Drug-induced lupus erythematosus.


Mitogenic activity of the jack bean (Canavalia ensiformis) with rabbit peripheral blood lymphocytes.


In vitro delayed (cellular) hypersensitivity in multiple sclerosis to central nervous system antigens.


Studies on the lymphocytes in the intestinal epithelium of the chicken. IV. Effect of bursectomy.


Antigenicity of polypeptides (poly-alpha-amino acids). Distribution of sheep antibodies to polymers of alpha-L-amino acids of varying net electrical charge and lysozyme.


The Chattanooga school children study: effects of community exposure to nitrogen dioxide. II. Incidence of acute respiratory illness.


Nitrogen dioxide and nitric oxide in non-urban air.


Fluorescent tracer studies in pollutant transport in the San Francisco Bay area.


Oxidant levels over San Francisco bay and adjacent land stations.


Note on Ontario's air pollution index and alert system.


Atmospheric aerosol composition and concentration as a function of particle size and of time.


Effects of fluorides on man.

Effects of fluorides on man.


Good management includes effective safety programs.


Cannery wastewater treatment by high-rate spray on grassland.


Removal of organic nitriles from wastewater systems.


Digestion fundamentals applied to digester recovery--two case studies.


Chemical control of tree roots in sewer lines.


Mental load, heart rate and heart rate variability.


Psychological and physiological reactions to noise of different subjective valence (TTS and EMG).


On Usnadze's volume illusion.

On Usnadze's volume illusion.


The "rete mirabile" in man.

The "rete mirabile" in man.


Results of the surgical treatment of intracranial arteriovenous malformations.


The late management of acute popliteal occlusion.


Iliac artery aneurysm presenting as acute ilio-femoral vein occlusion.


The present status of replantation of amputated extremities. Indications and technical considerations.


A special chamber to facilitate intra-aortic balloon pumping for circulatory assistance.


Aorto-iliac occlusive disease and horseshoe kidney. Review of the literature and case report.


Toward a cognitive theory of psychomotor intervention.


Some effects of isometric exercise on muscular strength, body-image perception, and psychiatric symptomatology in chronic schizophrenics.


Report of a meeting of the AMA Committee on Exercise and Physical Fitness.


Effects of neck position on patellar tendon reflex latency.


Cardiac reconditioning at the university of San Francisco.


A proposal for training educable mentally retarded adolescents and adults for roles as physical education, recreation aides and associates.


Fatigue.

Fatigue.


Prediction of dynamic strength from static strength in hip and knee extension.


Interlaboratory evaluation of the reliability of blood lead analyses.


Comparison of particle size determined with the Coulter counter and by optical microscopy.


Effects of age on work capacity.

Effects of age on work capacity.


Inhalation toxicology and lung edema receptor sites.


Evaluation of some parameters affecting the collection and analysis of midget impinger samples.


Comparison of cyclone and horizontal elutriator size selectors.


Evaluation of coproporphyrin in urine from workers exposed to lead.


The lack of heatstroke in European miners working in hot climates.


Sampling and determination by ultraviolet absorption of oil mists and solvent vapors.


Survey of monument industry in Ohio.

Survey of monument industry in Ohio.


The prenatal development of the human femur.


Hygienic guide series. Ethylene glycol monomethyl ether (2-methoxyethanol).


Influence of chemical state of cerium-144 on its metabolism following inhalation by mice.


A micromethod for mercury.

A micromethod for mercury.


The fate of isologous, homologous and heterologous ferritin molecules in the rat.


Current status of laser threshold guides.


An evaluation of the basic pattern of connections between the limbic system and the hypothalamus.


Analytical guides. Acrylonitrile.

Analytical guides. Acrylonitrile.


Cirrhosis of the liver following viral hepatitis, a twenty-year mortality follow-up.


Ergonomics guide to manual lifting.

Ergonomics guide to manual lifting.


Use of attenuated live mumps virus vaccine during a "virgin soil" epidemic of mumps on St. Paul Island, Alaska.


Experimental infection of ponies with the Hong Kong variant of human influenza virus.


Analytical guides. Atomic absorption spectrophotometry.


Cleaning of mercury and silicone grease contaminated glassware.


Calibration of high-volume samplers.

Calibration of high-volume samplers.


A simple method to collect air samples in a plastic bag.


Hygienic guide series. Pentachlorophenol and sodium pentachlorophenate.


Antiarrhythmic actions of coronary vasodilator agents papaverine, dioxyline and verapamil.


Solvent vapor analysis using activated charcoal tubes.


A study on the cardiac actions of some polyene antibiotics.


A comparison of the gastrointestinal absorption profiles of theophylline from various vehicles.


Anticonvulsant drugs and self-stimulation rates in rats.


Studies on myocardial catecholamines related to species ages and sex.


The effect of aminophylline on renal hemodynamics and sodium excretion.


Interaction of sympathomimetic amines with alpha-receptors and 5-hydroxytryptamine receptors in rabbit aorta.


Antibiotics and ureter. 3. Chloramphenicol.


Fluctuations in the hypoglycaemic effect of Poterium spinosum L. (Rosaceae).


The effects of phenobarbital on exocrine pancreas.


Effect of phenformin on induced hypercholesterolemia.


A nerve-chamber bioassay of some new and clinically used compounds.


Effects of ethyl alcohol upon spinal cord neurones.


Environments of individual tyrosines in protein. Differences in rates of mono- and diiodination.


Cardiovascular effects of chloralose in catecholamine-depleted or receptor-blocked dogs.


Chromatographic resolution of lysine-rich histones unaffected by phosphatase or ribonuclease treatment.


Circular dichroism of single- and double-peaked phycoerythrin: mercurial-induced changes.


Differences in amino acid sequence between dolphin and sperm whale myoglobins.


Differences between the association of the genetic variants B, C and D of alpha S-1-casein.


Optical rotatory dispersion and circular dichroism spectra of phytochrome.


Multiple hemoglobins in the golden hamster.


State hospital associations.

State hospital associations.


Long-range planning.

Long-range planning.


The child health associate.

The child health associate.


Philosophy and practice.

Philosophy and practice.


New forms system.

New forms system.


Hospital pharmacy systems.

Hospital pharmacy systems.


Laboratory furniture selection.

Laboratory furniture selection.


Reducing nursing turnover.

Reducing nursing turnover.


Research shows lack of supervisor influence in nursing turnover patterns.


Military flight meals.

Military flight meals.


Batch cooking.

Batch cooking.


Vacuum scrubbers.

Vacuum scrubbers.


Enzyme laundry products.

Enzyme laundry products.


Silicone injection of joints. Experimental and clinical trials.


Selective gastric vagotomy. Anatomical, experimental and clinical observations.


On the use of Biobond in the treatment of cerebrospinal rhinorrhea and frontobasal fistula.


Nonfunctioning adrenocortical carcinoma.


Septic infarction of the kidney due to Staphylococcus aureus.


Subhepatic biliary cyst with thoracic perforation following cholecystectomy.


Treatment of chronic hematogenous osteomyelitis. A clinical series of 65 cases.


Tubercular cholecystitis presenting as subacute intestinal obstruction.


Surgical treatment of "collar-stud" orbital abscess.


Acquired prostaglandin E2 (medullin) deficiency as the cause of oliguria in acute tubular necrosis. A hypothesis.


Red cell osmotic fragility in experimental murine leukemia.


Results of total correction of tetralogy of Fallot in twentyone cases.


The heart as contributor of plasminogen activator to the blood.


Anticardiac antibodies in ischaemic heart disease.


Childhood urolithiasis in northern Israel.


Progressive heart block in a case of scleroderma.


Chronic thallium poisoning.

Chronic thallium poisoning.


Studies on the enzymes of hydatid cyst fluid. Quantitative determination of enzymes present in echinococcus granulosus cyst fluid.


Leptospiral agglutinating factor in turtles. Further studies on fractionated sera.


Recurrent cholangitis caused by echinococcosis of the biliary passages.


Gastrointestinal side effects of tetracycline and its possible prevention by antifungal compounds.


Studies on microcirculation for the detection of microangiopathy in diabetes mellitus.


Comparative evaluation of antifungal agents in the prevention of tetracycline-induced candidiasis of gastrointestinal tract.


Serum and pleural fluid lactic dehydrogenase activity in the differential diagnosis of pleural effusions.


First isolation of Leptospira Icterohaemorrhagiae from man in Israel. With Remarks on the paradoxical reaction in the serodiagnosis of leptospirosis.


Effect of dipyrone (Novalgin) on haemopoietic system.


Effect of medial forebrain bundle lesions on adrenocortical responses to photic stimulation.


Bilirubinometry of jaundiced serum exposed to light: Malloy-Evelyn vs. direct spectrophotometric technique.


Inactivation of influenza and Newcastle disease viruses by oxidized spermine.


Presence of binding site for alpha-amylase and of masking protein for this site on mycelial cell wall of Aspergillus oryzae. Mycelial cell wall of Aspergillus oryzae M-13 grown in an alpha-amylase-forming medium could not bind alpha-amylase (Taka-amylase A, EC 3.2.1.1). However, by treatment with 1.0 n NaOH at 100 C for 30 min, the wall gained the ability to bind alpha-amylase. This phenomenon was caused by removal of a factor (designated as masking factor) which masked the binding site for alpha-amylase. The masking factor was purified as a preparation giving a single peak in both ultracentrifugation (1.6S) and by gel electrophoresis (M(BPB), 1.0). Approximately 20 mug of the purified factor, bound to 10 mg of the alkali-treated mycelial cell wall, prevented the binding of approximately 100 mug of alpha-amylase or released approximately 100 mug of alpha-amylase which previously was bound to the alkali-treated wall. These findings indicate that the factor has much higher affinity than alpha-amylase for the binding site on the mycelial wall. The masking factor was inducibly formed accompanying the secretion of alpha-amylase.


Regulation of pectate lyase synthesis in Pseudomonas fluorescens and Erwinia carotovora. Inducible synthesis of extracellular pectate lyase occurs in Erwinia carotovora, a bacterial soft-rot pathogen of plants, and, to a lesser extent, in a nonpathogenic isolate of Pseudomonas fluorescens. A combination of pectin and a heat-labile factor in fresh potato tissue or acetone powders of the tissue provided the best carbon source for induction. Yields of inducible pectate lyase were much greater than those usually reported. The pathogen, but not the saprophyte, produced a small amount of constitutive enzyme when grown on glucose. The relatively low level or absence of constitutive synthesis in these bacteria did not result from catabolite repression. Attempts were made to relieve any existing catabolite repression by restricting growth through slow feeding of glucose or by growing the organisms on glycerol. These conditions did not significantly alter the differential rate of lyase synthesis compared with changes observed in the presence of inducers. Previous growth history did not affect induction in the pathogen. However, P. fluorescens previously cultured on glucose required 10 to 20 generations of growth on inducing medium before appreciable lyase synthesis occurred. Differences between the pathogen and nonpathogen suggest that regulation of pectate lyase synthesis is related to pathogenicity of soft-rot bacteria.


Metabolism of the glycosyl diglycerides and phosphatidylglucose of Staphylococcus aureus. A glucose containing lipid, phosphatidylglucose (probably 3-sn-phosphatidyl-1'-glucose) and a lipid tentatively identified as phosphatidylethanolamine have been characterized in the lipids of Staphylococcus aureus. These lipids together comprise less than 2% of the total phospholipids of exponentially growing S. aureus and accumulate to 14% of the total phospholipid in stationary-phase cells. These lipids lost no (32)P when cells grown with H(3) (32)PO(4) were transferred to nonradioactive medium during the exponential growth phase. This was in marked contrast to the other phospholipids which lost (32)P rapidly. The loss of (32)P from phosphatidic acid and cardiolipin in exponentially growing cells was biphasic, suggesting heterogeneity of phospholipid phosphate metabolism. The mono- and diglucosyl diglycerides showed a rapid loss of (14)C-glucose during growth in nonradioactive medium but no loss of (14)C from the fatty acids of these lipids. The (14)C in the glucose and fatty acids of the glucosyl diglycerides was derived from glucose.


Characterization of a Staphylococcus aureus bacteriocin. The bacteriocin produced by a strain of Staphylococcus aureus has been isolated and designated staphylococcin (414), and a study was made of its chemical, physical, and biological properties. The staphylococcin is released in appreciable quantities after breakage of the cells and can be purified through differential centrifugation and column chromatography. In the native state, it appears to be a lipoprotein-carbohydrate complex with a molecular weight in excess of 200,000. The complex can be dissociated by sodium dodecyl sulfate into smaller subunits which retain activity. The gross chemical and physical properties of the bacteriocin closely resemble those ascribed to certain preparations of cell membranes. Staphylococcin (414) is not a lytic enzyme like lysostaphin and does not have the same spectrum of activity. Like other bacteriocins from gram-positive microorganisms, it does not inhibit any gram-negative bacteria, but does inhibit several other genera.


Carbon monoxide-reacting pigment from Desulfotomaculum nigrificans and its possible relevance to sulfite reduction. The separation of an autoxidizable brown pigment, P582, from Desulfotomaculum nigrificans is described. It reacted with Na(2)S(2)O(4) and was characterized by absorption maxima in the oxidized state at 392, 582, and 700 nm. In the presence of Na(2)S(2)O(4), P582 formed complexes with CO and, under alkaline conditions, pyridine. There was no reaction with cyanide. The molecular weight of P582 was approximately 145,000, and the purest preparations contained Fe, Zn, and acid-labile sulfide but not Cu, Mo, or Mn. Preparations of P582 catalyzed the reduced methyl viologen (MVH)-linked reduction of sulfite, hydroxylamine, and nitrite but not of sulfate, thiosulfate, or nitrate. Reduced pyridine nucleotides did not substitute for MVH. A major product of the MVH-sulfite reaction was sulfide. CO partially inhibited the enzymatic activities. Sulfite, hydroxylamine, and nitrite and CO caused changes in the spectrum of Na(2)S(2)O(4)-reduced P582. Fe(2+)-chelating reagents reacted with part of the Fe of P582 and caused partial losses of labile sulfide and enzymatic activity. The spectral and CO-reacting properties of P582 were, however, unaffected by chelating agents. The reaction between P582 and chelating agents was stimulated by reducing agents.


Mechanism of action of the antifugal agent polyoxin D. The antibiotic polyoxin D was shown to inhibit the incorporation of (14)C-glucosamine into cell wall chitin in Neurospora crassa at levels which were comparable with those required for inhibition of fungal growth. At the same time, the antibiotic increased the accumulation of a nucleotide, which was identified as uridine diphosphate (UDP)-N-acetylglucosamine, indicating inhibition of chitin synthesis. Chitin synthetase (UDP-N-acetylglucosamine: chitin N-acetylglucosaminyl transferase, EC 2.4.1.16) of N. crassa was found to be strongly inhibited by polyoxin D, as determined by the transfer of (14)C-N-acetylglucosamine from (14)C-UDP-N-acetylglucosamine to the particulate fraction. The inhibition was competitive with respect to UDP-N-acetylglucosamine and specific for chitin synthetase. The K(i) for polyoxin D in the reaction was 1.40 x 10(-6)m, and the K(m) for UDP-N-acetylglucosamine was 1.43 x 10(-3)m. The formation of osmotically sensitive, protoplast-like structures, when the fungus Cochliobolus miyabeanus was grown in the presence of polyoxin D, also suggested that the primary site of action of polyoxin D was in the formation of cell wall structures.


Repression of acetyl-coenzyme A carboxylase by unsaturated fatty acids: relationship to coenzyme repression. It has been reported that the level of d-biotin in the growth medium of Lactobacillus plantarum regulates the synthesis of apoacetyl-coenzyme A (CoA) carboxylase; high levels cause repression, and deficient levels effect derepression. In this study, evidence has been obtained which suggests that coenzyme repression by biotin is an indirect effect; i.e., biotin regulates the synthesis of unsaturated fatty acids which are the true repressors of the acetyl-CoA carboxylase. This was observed in an experiment in which long-chain unsaturated fatty acids were added to media containing deficient, sufficient, or excess levels of d-biotin. In every case, independently of the biotin concentration for growth, the unsaturated fatty acids caused a severe repression of the carboxylase. Saturated fatty acids were without effect. The level of oleic acid required to give maximal repression was 50 mug/ml. The free fatty acids had no adverse effect on the activity of the cell-free extracts nor on the permeation of d-biotin into the cell. Saturated and unsaturated fatty acids decreased the rate of holocarboxylase formation from d-biotin and the apoacetyl-CoA carboxylase in the extracts. It is concluded that there are at least three mechanisms that control the acetyl-CoA carboxylase in this organism: (i) indirect coenzyme repression by d-biotin, (ii) repression by unsaturated fatty acids, and (iii) regulation of the activity of the holocarboxylase synthetase by both saturated and unsaturated fatty acids.


Occurrence of Crithidia factors and folic acid in various bacteria. Crithidia factors and folic acid were found to be widely distributed in culture fluids and in cells of 27 species of bacteria, when cultured under aerobic conditions into the stationary phase. Most bacteria excreted more Crithidia factors and folic acid than they retained in their cells. One Crithidia factor produced by Serratia indica and one produced by Bacillus cereus differed from biopterin in their chromatographic behavior. The factor excreted by S. indica appeared to be a 2-amino-4-hydroxy-6-substituted pteridine on the basis of KMnO(4) oxidation and ultraviolet absorption spectra. One of the folate compounds excreted by this organism was shown to be identical to 5,10-methylidynetetrahydrofolic acid by bioautography.


Transport of folinate and related compounds in Pediococcus cerevisiae. The properties of folinate and 5-methyltetrahydrofolate (5-CH(3)-H(4)PteGlu) transport mechanism of Pediococcus cerevisiae were studied. The uptake was dependent on temperature, pH (optimum for both compounds at pH 6.0), and glucose. Iodoacetate, potassium fluoride, and sodium azide inhibited the uptake. 5-CH(3)-H(4)-PteGlu was apparently not metabolized but folinate was metabolized. Metabolism of folinate was reduced by preincubation of cells with fluorodeoxyuridine. The transport system for folinate and 5-CH(3)-H(4)PteGlu were specific for the l-isomers. Pteroylglutamate, aminopterin, and amethopterin did not interfere with the uptake. Tetrahydrofolate competed with the uptake of folinate. The transport of folinate and 5-CH(3)-H(4)PteGlu at 37 C conformed to Michaelis-Menten kinetics; apparent K(m) for both compounds was 4.0 x 10(-7)m, and the V(max) for folinate was 1.0 x 10(-10) moles per min per mg (dry weight) and for 5-CH(3)-H(4)PteGlu it was 1.6 x 10(-10) moles per min per mg (dry weight). Both compounds accumulated in the intracellular pool at a concentration about 80- to 140-fold higher than that in the external medium. Folinate inhibited competitively the uptake of 5-CH(3)-H(4)PteGlu with a K(i) of 0.4 x 10(-7)m. Unlike 5-CH(3)-H(4)PteGlu, which accumulated only at 37 C, folinate was also taken up at 0 C by a glucose- and temperature-independent process, which was not affected by the metabolic inhibitors mentioned above. Since at 0 C the intracellular concentration of folinate was also considerably higher than the external, binding of the substrate to some cellular component is assumed. The finding of an efficient transport system for l-5-CH(3)-H(4)PteGlu is of special interest, since this compound has no growth-promoting activity for P. cerevisiae.


Conditional mutants of meiosis in yeast. Three temperature-sensitive mutants, spo1-1, spo2-1, and spo3-1, were characterized with respect to their behavior in sporulation medium at a restrictive temperature. The time of expression of the functions defective in the mutants was determined by temperature-shift experiments during the sporulation process. In addition, each mutant was examined for the following: (i) its ability to undergo the nuclear divisions of meiosis; (ii) deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and protein synthesis; (iii) protein turnover; and (iv) colony-forming ability after exposure to sporulation medium. Mutant spo1-1 is defective in a function which confers a temperature-sensitive period which extends over 32% of the sporulation cycle. The temperature-sensitive period of mutant spo2-1 occupies 34% of the cycle, whereas the temperature-sensitive period of mutant spo3-1 extends over 2% of the sporulation cycle. Cytological evidence indicates that all three mutants initiate but do not complete the meiotic nuclear divisions. The DNA content of sporulation cultures of mutants spo1-1 and spo3-1 did not increase to the wild-type level; DNA synthesis in spo2-1 was normal. All three strains exhibit a loss of colony-forming ability during incubation in sporulation medium at the restrictive temperature. RNA and protein synthesis and protein turnover occur in the mutants.


Formation of bacteriolytic enzymes in batch and continuous culture of Staphylococcus aureus. The formation of bacteriolytic enzymes of Staphylococcus aureus, with special reference to strain M18, was investigated under a variety of conditions. The bacteriolytic activity was tested by using whole cells of Micrococcus lysodeikticus as a substrate. Complex media were required for production, and a Casein Hydrolysate-Yeast Extract medium (CCY(I)) was superior to Brain Heart Infusion and Trypticase Soy Broth. The optimal pH level for production was 7.0. Effective oxygenation and exchange of the beta-glycerophosphate of the CCY(I) medium for glucose increased the rates of growth and autolysis and the rate of appearance of extracellular bacteriolytic enzymes. However, the extracellular lytic activity decreased more rapidly at the end of the growth period than under the standard culture conditions. The appearance of inhibitor(s), probably derived from autolysis, might be responsible for this rapid decrease. The highest yields were obtained in a continuous process in which the activity was almost twice that of batch cultures grown under the same conditions. The bacteriolytic activity produced in continuous culture had a considerably increased stability in the purification process. The advantage of producing unstable bacterial proteins in continuous culture under controlled growth conditions is discussed.


Utilization of glucose in heterotrophic media by Thiobacillus intermedius. The growth yield of Thiobacillus intermedius is greater in glucose-yeast extract or glucose-casein hydrolysate broth than in comparable media without glucose. The quantity of glucose utilized in the glucose-supplemented media is much greater than the increase in cell yield observed relative to the unsupplemented media. Addition of glucose to cell-free extracts of glucose-yeast extract or glucose-casein hydrolysate grown cells results in the reduction of endogenous cytochrome c. Thus, in these media, glucose serves as a source of energy. This is in contrast to thiosulfate-glucose broth in which glucose provides only cell carbon. The presence of thiosulfate in glucose-casein hydrolysate broth results in a marked decrease in glucose consumption. Cytochrome c in extracts of cells grown in this medium is not reduced by glucose addition. The data suggest that thiosulfate prevents the utilization of glucose for energy generation. The final growth yield in glucose-casein hydrolysate broth is directly proportional to the initial glucose concentration, although not all the glucose was utilized even at the lowest concentration tested. This effect may be due to an inefficient glucose transport in this organism.


Regulation of glucose metabolism in Thiobacillus intermedius. Glucose-yeast extract or glucose-casein hydrolysate-grown Thiobacillus intermedius cells, which use glucose for energy generation, possess high specific activities of the Entner-Doudoroff pathway and related enzymes, 6-phosphogluconate dehydrase, 2-keto-3-deoxy-6-phosphogluconate aldolase, glucokinase, and glucose-6-phosphate dehydrogenase, but low activities of enzymes unique to the pentose shunt and Embden-Meyerhof pathways. Although the synthesis of the latter enzymes remains largely unaffected by the growth environment, that of the former is stimulated by glucose. Radiorespirometric measurements demonstrate an early and parallel respiration of glucose carbon atoms one and four in glucose-casein hydrolysate broth. It is concluded that the Entner-Doudoroff pathway performs an energetic role in glucose metabolism by T. intermedius with the pentose shunt and Embden-Meyerhof pathways functioning mainly in biosynthesis. The presence of thiosulfate in the growth medium inhibits the synthesis of the Entner-Doudoroff pathway and related enzymes. In addition, both thiosulfate and glucose inhibit the synthesis of the Krebs cycle enzymes, nicotinamide adenine dinucleotide phosphate-linked isocitrate and alpha-ketoglutarate dehydrogenases. Thus, repression of enzymes is of significance in the adaptation of T. intermedius to its nutritional environment. The activity of glucose-6-phosphate dehydrogenase of T. intermedius is inhibited by adenosine triphosphate. Such a control could afford the organism a mechanism to regulate the flow of glucose into major energetic and biosynthetic routes.


Phospholipid metabolism during penicillinase production in Bacillus licheniformis. During membrane-bound penicillinase production, Bacillus licheniformis forms vesicles and tubules that do not appear in the absence of penicillinase production. The major lipids of B. licheniformis were shown to be phospholipids. The proportions, metabolism, and the total phospholipid per cell were shown to be essentially the same in the uninduced control, induced and constitutive penicillinase forming cells during both the exponential and stationary growth phases. Membrane phospholipids were not secreted into the medium during penicillinase formation. In the shift from the exponential to the stationary growth phase, there was an accumulation of phosphatidyl glycerol and a marked decrease in cardiolipin. These two lipids had the most active turnover of their phospholipid phosphate of all the lipids studied.


Enzymes of the tryptophan pathway in Acinetobacter calco-aceticus. All enzymes of the tryptophan synthetic pathway were detectable in extracts from wild-type Acinetobacter calco-aceticus. The levels of these enzymes were determined in extracts from a number of auxotrophs grown under limiting tryptophan. In each case only anthranilate synthetase was found to be present in increased amounts, whereas the specific activities of the remaining enzymes remained unchanged and unaffected by the tryptophan concentration. Derepression of anthranilate synthetase was found to occur as the concentration of tryptophan became limiting. Anthranilate synthetase and phosphoribosyl transferase activities are both feedback-inhibited by tryptophan. Molecular weight determination carried out by gel filtration and zonal centrifugation in sucrose revealed that all the enzymes are less than 100,000, and no molecular aggregates of these enzymes were detected. The data indicate that tryptophan synthesis in Acinetobacter is regulated both by feedback inhibition of the first two enzymes of the pathway and by repression control of anthranilate synthetase.


Isolation and identification of echinenone from Micrococcus roseus. An orange carotenoid from Micrococcus roseus was purified by solvent partitioning followed by column and thin-layer chromatography. Absorption spectra, chromatographic mobility, and partition coefficient suggested that the pigment was echinenone (4-keto-beta-carotene). Reduction yielded a pigment with the spectral and polar properties of isocryptoxanthin (4-hydroxy-beta-carotene), the expected product. The orange pigment and its reduction product co-chromatographed with the respective authentic pigments, confirming the original pigment as echinenone. To our knowledge echinenone has not been identified previously as a bacterial pigment.


Ribonucleic acid synthesis during microcyst formation in Myxococcus xanthus: characterization by deoxyribonucleic acid-ribonucleic acid hybridization. The technique of deoxyribonucleic acid-ribonucleic acid (RNA) hybridization was used to compare the RNA synthesized during vegetative growth and microcyst formation in Myxococcus xanthus. All classes of RNA, including ribosomal RNA, were synthesized during microcyst formation. The results indicate that the ribosomal RNA synthesized during microcyst formation was indistinguishable from that made during vegetative growth. Hybridization competition experiments demonstrated that certain messenger RNA species are synthesized only during vegetative growth, whereas others are synthesized only during microcyst formation. The synthesis of a new species of RNA polymerase does not appear to be responsible for differential transcription during morphogenesis in M. xanthus since the rifampicin sensitivity of transcription was conserved during microcyst formation.


Penicillinase (beta-lactamase) induction in Bacillus licheniformis under Pseudogratuitous conditions by 2-(2'-carboxyphenyl)-benzoyl-6-aminopenicillanic acid. Induction of penicillinase (beta-lactamase) in Bacillus licheniformis 749 by 2-(2'-carboxyphenyl)-benzoyl-6-aminopenicillanic acid (CBAP) was examined, since this compound was reported to be a gratuitous inducer of penicillinase in Staphylococcus aureus. The specific activity of enzyme optimally induced by CBAP is slightly more than that formed in response to cephalosporin C and threefold the level induced by benzylpenicillin. The optimal inducer concentration of CBAP was not inhibitory toward the growth of penicillinase-deficient mutants, unlike benzylpenicillin or cephalosporin C which showed marked toxicities. CBAP is hydrolyzed by the Bacillus penicillinase, but as indicated by its "physiological efficiency" (V(max)/K(m)), CBAP is a poor substrate at low concentrations. At very high concentrations, CBAP inhibited benzylpenicillin hydrolysis. The overall effectiveness of CBAP as an inducer can be attributed to its low "physiological efficiency" which enables the use of nontoxic levels of CBAP for induction without its rapid hydrolysis. Although CBAP is not a true gratuitous inducer, operationally it approaches gratuity for induction of B. licheniformis penicillinase better than other known inducers.


Cellular control of the synthesis and activity of the bacterial luminescent system. In bioluminescent bacteria growing in shake flasks, the enzyme luciferase has been shown to be synthesized in a relatively short burst during the period of exponential growth. The luciferase gene appears to be completely inactive in a freshly inoculated culture; the pulse of preferential luciferase synthesis which occurs later is the consequence of its activation at the level of deoxyribonucleic acid transcription which is attributed to an effect of a "conditioning" of the medium by the growing of cells. Although cells grown in a minimal medium also exhibit a similar burst of synthesis of the luminescent system, the amount of synthesis is quantitatively less, relative to cell mass. Under such conditions, added arginine results in a striking stimulation of bioluminescence. This is attributed to a stimulation of existing patterns of synthesis and not to induction or derepression per se.


Protein difference between competent and noncompetent cultures of a group H Streptococcus. Acidified phenol extracts prepared from competent cultures of a group H Streptococcus strain Wicky made competent with competence factor derived from cultures of another group H Streptococcus, strain Challis, showed a difference in polyacrylamide-gel protein patterns when compared to extracts prepared from noncompetent cultures of strain Wicky. The prominent single protein band difference did not appear when Wicky cells were simultaneously treated with competence factor and chloramphenicol, an inhibitor of the development of competence. Chloramphenicol had no effect on transformation nor the appearance of the "new" protein band when added to fully competent cells. This new protein, which is associated with the appearance of competence, seems to be synthesized as a result of induction by competence factor; its exact role, however, is as yet unknown.


Changes in membrane lipid composition in exponentially growing Staphylococcus aureus during the shift from 37 to 25 C. Lowering the temperature of growth of Staphylococcus aureus from 37 to 25 C decreased the growth rate and induced changes in the composition of the membrane lipids. Changes in lipid composition also occur in the transition between exponential and stationary growth phases at one temperature. To isolate the effects of lowering the temperature, exponentially growing S. aureus was abruptly switched from 37 to 25 C by transfer to cooler medium. Exponential growth continued at 25 C without a lag period but with a threefold increase in doubling time. In the period of exponential growth at suboptimal temperature, there was essentially no change in the fatty acid composition of the lipids, little change in the vitamin K(2) composition with perhaps a slight increase in the total level, and essentially no change in the phospholipid composition, but a marked stimulation of the synthesis of the rubixanthins. Growth of cells at 25 C was much more sensitive to the inhibition of rubixanthin formation by mixed-function oxidase inhibitors than cells growing at 37 C, suggesting some function for the rubixanthins at suboptimal temperatures. The striking increases in the proportions of monoenoic fatty acids observed at lowered growth temperatures in many biological systems are not detected in S. aureus.


Anaerobic growth of purple nonsulfur bacteria under dark conditions. Purple nonsulfur photosynthetic bacteria were cultured anaerobically in the absence of light by a modification of the Hungate technique. Growth was slow and resembled that of fastidious anaerobes; on yeast extract-peptone-agar medium, each cell produced about 16 descendants in 15 to 20 days. Growth was stimulated by addition of ethyl alcohol, acetate and H(2), or pyruvate and H(2). Cells grown in the presence of pyruvate and H(2) produced acetate and CO(2); each cell produced approximately 10 descendants in 24 hr under anaerobic, dark conditions. Spectrophotometric evidence obtained from cells which were the product of five generations suggests no difference between the bacteriochlorophyll and carotenoids synthesized by cells grown anaerobically under dark or light conditions. Likewise, the ultrastructure of the photosynthetic apparatus in cells grown anaerobically in the dark and in the light appears similar.


Polyphasic taxonomy of the genus Vibrio: polynucleotide sequence relationships among selected Vibrio species. Polynucleotide relationships among selected Vibrio species were examined by means of deoxyribonucleic acid (DNA) reassociation reactions and chromatography on hydroxyapatite. Relative levels of intraspecific DNA duplex formation (V. cholerae-V. cholerae and V. parahaemolyticus-V. parahaemolyticus) were found to be high at 60 C (>80%), and only minimally reduced at 75 C. Interspecific DNA duplexes between V. cholerae DNA and that of the non-cholera vibrios also exhibited high relative levels of formation at 60 C (>80%) and, with one exception, were only slightly reduced at 75 C. The thermal stability of these duplexes formed at 60 or 75 C was virtually identical to that of homologous V. cholerae DNA duplexes. The degree of reassociation and the thermal stability of V. cholerae-non-cholera vibrio DNA duplexes suggests relatively little evolutionary divergence in these organisms. In all other interspecific DNA reassociation reactions, only low levels of DNA duplex formation were noted at 60 C (<25%), and these were drastically reduced (>50%) at 75 C. The degree of nucleotide sequence divergence indicated by these reactions suggests that these Vibrio species are not significantly related to V. cholerae or V. parahaemolyticus. Reassociation reactions between V. cholerae DNA and the DNA of V. parahaemolyticus indicated these species were not significantly related to each other.


Polyphasic taxonomy of the genus vibrio: numerical taxonomy of Vibrio cholerae, Vibrio parahaemolyticus, and related Vibrio species. A set of 86 bacterial cultures, including 30 strains of Vibrio cholerae, 35 strains of V. parahaemolyticus, and 21 representative strains of Pseudomonas, Spirillum, Achromobacter, Arthrobacter, and marine Vibrio species were tested for a total of 200 characteristics. Morphological, physiological, and biochemical characteristics were included in the analysis. Overall deoxyribonucleic acid (DNA) base compositions and ultrastructure, under the electron microscope, were also examined. The taxonomic data were analyzed by computer by using numerical taxonomy programs designed to sort and cluster strains related phenetically. The V. cholerae strains formed an homogeneous cluster, sharing overall S values of >/=75%. Two strains, V. cholerae NCTC 30 and NCTC 8042, did not fall into the V. cholerae species group when tested by the hypothetical median organism calculation. No separation of "classic" V. cholerae, El Tor vibrios, and nonagglutinable vibrios was observed. These all fell into a single, relatively homogeneous, V. cholerae species cluster. V. parahaemolyticus strains, excepting 5144, 5146, and 5162, designated members of the species V. alginolyticus, clustered at S >/=80%. Characteristics uniformly present in all the Vibrio species examined are given, as are also characteristics and frequency of occurrence for V. cholerae and V. parahaemolyticus. The clusters formed in the numerical taxonomy analyses revealed similar overall DNA base compositions, with the range for the Vibrio species of 40 to 48% guanine plus cytosine. Generic level of relationship of V. cholerae and V. parahaemolyticus is considered dubious. Intra- and intergroup relationships obtained from the numerical taxonomy studies showed highly significant correlation with DNA/DNA reassociation data.


Morphogenesis of cysts in Azotobacter vinelandii. Cultures of Azotobacter vinelandii were induced to encystment with beta-hydroxybutyrate. The morphological events in the transition from cell to cyst were observed by electron microscopy of thin sections. Upon induction of encystment, cells became rounded and nonmotile. The exine coat developed by the continuous excretion of membranous components into the capsule surrounding the cell.


Scanning-beam electron microscopy of Mycoplasma pneumoniae. The morphology and the existence of a growth cycle of Mycoplasma pneumoniae have not been clearly established. There is disagreement as to whether this organism exists as a spherical or filamentous form, and whether it progresses from filamentous to spherical forms as the organism ages. A scanning-beam electron microscope (SEM) was utilized to provide detailed observations of the cycle of morphological changes during growth phases of M. pneumoniae. Cultures of cells grown and fixed in liquid suspension displayed morphological changes from spherical to filamentous and then to larger round forms. After 8 hr to 2 days of growth (phase I), spherical forms and aggregates were revealed. Two- to 6-day-old growth (phase II) was composed of both straight and branching filaments with bulbous elements situated at intervals along their lengths, and microcolonies composed predominantly of intertwined filaments. Six- to 10-day-old growth (phase III) was characterized by flattened, spherical organisms larger than those observed in phase I, occasional membranes or ghosts, and a paucity of aggregates or microcolonies. Thus, stereo-scan electron microscopic studies suggest that M. pneumoniae undergoes an orderly and sequential metamorphosis during its life cycle.


Fine structure of Thermus aquaticus, an extreme thermophile. Electron microscopic studies using thin sections revealed that Thermus aquaticus has a structure similar to that of most other gram-negative bacteria. The cell envelope is tripartite: plasma membrane, thin middle layer, and a thicker and irregular outer layer. The outer layer appears to be joined to the plasma membrane by a series of connections and, when seen in tangential section, the outer layer appears as a series of parallel bands. The cell division mechanism resembles that of typical gram-negative bacteria. Large spherical bodies designated "rotund bodies" are formed as a result of the association of a number of separate cells. In this association the outer envelope layers of the cells fuse and pull away from the middle layer. The rotund body thus appears as a series of rods, usually lying in parallel around the periphery of the sphere, completely connected by means of the fused outer layer.


Segregational respiratory-deficient mutants of a "petite negative" yeast Schizosaccharomyces pombe 972h-. No viable respiratory-deficient mutants of Schizosaccharomyces pombe 972h(-) could be obtained by acriflavine and ethidium bromide treatments. These mutagens induce 15 to 70% of microcolonies which, after a growth-lag of a few days, further develop into normal, respiratory-competent colonies. These results suggest that unstable petites were induced. Segregational respiratory-deficient mutants resistant to cobalt sulfate inhibition were isolated. Some of these strains are deficient in cytochrome a + a(3) and respire at low rates. The morphology of their mitochondrial membranes is modified: either the cristae are absent or they show aberrant concentric or tubular structures. Segregational mutants resistant to the respiratory inhibitors, 2,4-dinitrophenol or decamethylene diguanidine, were obtained. Neither mitochondrial structure nor function seems to be modified in these mutants. A segregational mutant resistant to benzimidazole inhibition does not grow on glycerol, although neither growth on glucose nor respiration appear to be affected.


Biosynthesis of alpha-ketoglutarate by the reductive carboxylation of succinate in Bacteroides ruminicola. Experiments with growing cells and with cell-free extracts of Bacteroides ruminicola indicate that this anaerobic bacterium can synthesize alpha-ketoglutarate by a reductive carboxylation of succinate. When the organism was grown in medium containing succinate-1,4-(14)C, most of the radioactivity in cells was in the protein fraction and most of the (14)C in protein was in the glutamic acid family of amino acids (glutamate, proline, and arginine). When unlabeled succinate was added to culture medium containing glucose-U-(14)C, incorporation of radioactivity into the glutamic acid family of amino acids was greatly reduced. This supports the concept that succinate is an intermediate in synthesis of alpha-ketoglutarate. Cell-free extracts of the organism incubated with succinate-1,4-(14)C incorporated (14)C into amino acids and most of this was found in glutamate. The cofactors which stimulate glutamate synthesis from succinate by extracts from these cells appear to be similar to the factors that have been demonstrated with extracts from photosynthetic bacteria. The position of label in glutamate synthesized from succinate-1,4-(14)C, the probable absence of isocitric dehydrogenase, and studies with labeled citrate and with inhibitors of citric acid cycle enzymes support the concept of a reductive carboxylation of succinate as the only, or at least a major, mechanism for synthesis of alpha-ketoglutarate in this organism. This appears to be the first evidence for a net synthesis of alpha-ketoglutarate by this reaction in a nonphotosynthetic heterotrophic organism.


Control of morphogenesis in Geodermatophilus: ultrastructural studies. Geodermatophilus grows in two major forms, a nonmotile irregularly shaped aggregate of coccoid cells (C-form) and a motile budding rod (R-form). Morphogenesis can be controlled by an unidentified factor in Tryptose which is required for maintenance of the organism in the C-form and for differentiation of the R-form to the C-form. Morphogenetic events occur synchronously in the described system. Ultrastructural studies show that the major difference between C- and R-forms is in cells envelope structure. R-form cell walls consist of two layers, an inner transparent membranous layer (10 to 12.5 nm thick) and an outer dense diffuse layer (7.5 to 10 nm). In addition to these layers, the C-form has a thick fibrous layer (30 nm) over the dense layer. This layer appears to be a cementing substance which holds the coccoid cells together.


Cytochemical localization of catalase activity in yeast peroxisomes. Diaminobenzidine oxidation product occurred in peroxisomes and in the intracristate spaces of mitochondria. The reaction was inhibited only in peroxisomes when 3-amino-1,2,4-triazole was present, but cyanide and azide inhibited deposition in both kinds of organelles.


Mutagenic action of N-methyl-N'-nitro-N-nitrosoguanidine on Gaffkya tibisci at alkaline pH. Good yields of mutants were obtained by incubating cells of Gaffkya tibisci with N-methyl-N'-nitro-N-nitrosoguanidine in alkaline buffer. Nearly 50% of the survivors were auxotrophic mutants under the conditions described.


Impurity in a common growth medium component: presence of an isoflavonoid in samples of L-asparagine. An impurity, present in some samples of l-asparagine, gave genistein on acid hydrolysis. The contaminant (probably the glucoside of genistein) was metabolized by Mycobacterium phlei to genistein and prunetin.


Glycogen metabolism in Chlamydia-infected HeLa-cells. A difference in glycogen metabolism between two strains of Chlamydia agents was observed which can serve as a taxonomic marker to distinguish C. psittaci from C. trachomatis.


Properties of the amylase from Halobacterium halobium. Halobacterium halobium amylase had optimal activity at pH 6.4 to 6.6 in sodium beta-glycerophosphate buffer containing 0.05% NaCl at 55 C; Ca(2+) was not required. End products from amylose were maltose, maltotriose, and glucose. The amylase, which was devoid of transglucosylase activity, had a multichain attack mechanism.


Substrate inhibition of transglucosyl-amylase by maltose. Transglucosyl-amylase was inhibited by maltose when maltose served as a substrate. As a function of substrate concentration, the rates initially rose proportionately with increases of maltose levels until a maximal rate was attained. Further increases of maltose concentration decreased reaction rates. The attainment of a maximal rate with increasing substrate concentration and close correspondence between experimental and calculated rates indicated the involvement of ternary complex formation. Evidence also suggested that substrate inhibition is caused by maltose competing with water for the acceptor sites during complex formation.


Alterations of the respiratory system of Neurospora crassa by the mi-1 mutation. The respiratory mechanism of the cytochrome-defective mi-1 mutant of Neurospora crassa was partially characterized and compared with that of the wild type. Both systems of electron transport were linked to oxidative phosphorylation and showed similar characteristics with respect to substrates and components. The system operating in the cytochrome-deficient mi-1 mutant showed no characteristic alpha absorption bands of cytochromes a or b and was very unstable in vitro. The possibility that this system may be an altered form of the normal cytochrome electron transport chain which occurs in the wild type is discussed.


Heterogenomic recombinants from compatible nocardiae. Recombinants obtained from matings of Nocardia erythropolis x N. canicruria were tested for their genetic stability by comparing phenotypes from direct selection with the same population after unselected growth. Contraselective loci were employed in various combinations in order that all of the mapped characters might be subjected to unselected analysis. Some recombinant class types appeared as stable haploids, whereas others behaved as heterozygous diploids, segregating out new phenotypes. All regions of the parental genomes were found to be involved in segregation, implying that the entire mapped region can become merozygotic under standard mating conditions. On the basis of segregating phenotypes, the genetic potentials of these compatible nocardiae were ascertained as follows: the formation of a diploid with subsequent segregation of parental or haploid recombinant genomes or both; persistence of the diploid through many generations; continuing reassortment of genetic information by multiple matings between parental or recombinant organisms; and, very probably, second-round recombinations within the diploid. A considerable difference in the nuclear division time between the parental organisms was postulated to have significant effects on the nature of the unselected segregants.


Potassium uptake during microcyst formation in Myxococcus xanthus. The kinetics of (42)K uptake by Myxococcus xanthus during vegetative growth and microcyst formation were determined. In the medium studied, growing cells concentrated potassium about 100-fold, yielding an intracellular concentration of 147 mm. The influx of K(+) in growing cells was 17 +/- 3 pmoles of K(+)/cm(2) min. About 5 hr after induction of vegetative cells to microcysts, the K(+) influx decreased and the intracellular concentration fell. By 18 hr after induction, there was no measurable influx of K(+), and the intracellular concentration of potassium was less than 29 mm. There was, however, considerable binding of K(+) to the "surface" of microcysts. It is postulated that the greatly reduced intracellular concentration of potassium helps to maintain the microcyst in its dormant state and protects it against enzymatic break-down.


Accumulation of streptomycin-phosphate in cultures of streptomycin producers grown on a high-phosphate medium. A phosphorylated derivative of streptomycin accumulated in cultures of Streptomyces griseus ATCC 12475 and SC2376 grown on complex media containing an excess of inorganic phosphate (0.01 m). This compound did not accumulate significantly in the absence of added inorganic phosphate. The phosphorylated derivative did not inhibit growth of Bacillus subtilis or support growth of a streptomycin-dependent strain of Escherichia coli; however, incubation of the derivative with alkaline phosphatase gave a compound which was active with both systems. In the phosphorylated derivative, phosphate is esterified with an -OH group of the streptidine moiety of streptomycin. It is suggested that the phosphoryl group is introduced during biosynthesis of the streptidine moiety of streptomycin or by the action of streptomycin kinase on preformed streptomycin (or both), and subsequent dephosphorylation by streptomycin-phosphate phosphatase is inhibited by high concentrations of inorganic phosphate. A column chromatographic procedure for separation of streptidine-phosphate, streptomycin-phosphate, and streptomycin is described.


Histidine-mediated control of tryptophan biosynthetic enzymes in Neurospora crassa. The formation of the five tryptophan biosynthetic enzymes of Neurospora crassa was shown to be derepressed in histidine-starved cells. This histidine-mediated derepression was not due to a lowered intracellular concentration of tryptophan in these cells. Furthermore, histidine-mediated derepression of tryptophan enzymes was found to be coordinate and not subject to reversal by tryptophan of either exogenous or biosynthetic origin. The synthesis of tryptophan enzymes also was found to be coordinate in cells which were not histidine-starved. Although histidine is clearly involved in regulating the synthesis of tryptophan enzymes, it did not prevent either tryptophan-mediated derepression of tryptophan enzymes or indole-3-glycerol phosphate-mediated derepression of tryptophan synthetase.


Medical staff forum. To get the doctors into the act, take them out of the hospital.


Operating room forum. Inhalation therapy equipment may be reservoir for gram-negative bacteria.


Modern central service. How to store and maintain traction frames.


Modern pharmacy practice. Here are ways to get rid of unused drugs.


Modern food management. Labor turnover stems from poor guidance, petty gripes.


Electronic guardian watches hospital's mechanical systems.


One way to give doctors recognition: like everybody else, let them pay.


Lobbying may be useful and needed--if you know how and do it right.


Modern hospital purchasing. What purchasing must know: policies, procedures, prices.


Infant I.C.U.s save lives--but too many units may add cost and hamper growth.


Hospital must specify criteria for medical staff membership.


Hospital disaster plan meets 40 hour test during racial rioting.


Here's how in-service education works.

Here's how in-service education works.


The most promising manpower resource is the manpower that's already there.


Goodbye Science Journal.

Goodbye Science Journal.


Changes in Moskva River.

Changes in Moskva River.


Rashness that may pay off.

Rashness that may pay off.


NSF declared sole heir.

NSF declared sole heir.


New York children lack vitamin A.

New York children lack vitamin A.


Should palaeoanthropology be big science?


Impact of the laser.

Impact of the laser.


Accelerating somatic cell genetics.

Accelerating somatic cell genetics.


Genetics of somatic mammalian cells: linkage studies with human-Chinese hamster cell hybrids.


Field and laboratory observations of the crown-of-thorns starfish, Acanthaster planci.


Possible dopamine derivatives capable of crossing the blood-brain barrier in relation to Parkinsonism.


Sunlight-induced pyrimidine dimers in human cells in vitro.


New form of molecular defect in xeroderma pigmentosum.


Effects of an imprinting procedure on RNA polymerase activity in the chick brain.


Motion of spermatozoa in fluid streams.

Motion of spermatozoa in fluid streams.


Separation of a hyaluronate-free lubricating fraction from synovial fluid.


Hibernation and the production of glycerol in the Ichneumonidae.


Validity of the selective coefficient of a behaviour trait in hawkmoth larvae.


Skeletons of Lumbricus terrestris L. and Arenicola marina (L).


Structure of the nuclear pore in higher plants.


A congress is a congress is a congress.

A congress is a congress is a congress.


Biological membranes.

Biological membranes.


Follicle-stimulating hormone levels during pregnancy.


Endometrial hyperplasia in young women.

Endometrial hyperplasia in young women.


Urinary excretion of estrogens in women with hydatidiform mole and choriocarcinoma.


Application of the obstetric forceps.

Application of the obstetric forceps.


Placenta percreta with bladder invasion and massive hemorrhage. Report of a case.


Low-dose RISA for placental localization.


Gestational age and placental cellular replication.


Urinary estriol excretion before severe placental abruption.


Primary endometrial stromatosis of ovary. Report of a case.


Spontaneous rupture of gynandroblastoma during labor. Report of a case.


Influence of estrogen on the cholinesterase content of fetal brain.


Parental chromosomal aberrations associated with multiple abortions and an abnormal infant.


Cervical cytology and sequential birth control pills.


Personality factors in preeclampsia of pregnancy.


Luteal phase inadequacy. Its significance in infertility.


Anterior sacral meningocele as a gynecologic problem. Report of a case.


Central venous pressure in supine position during normal pregnancy. Comparative determinations during first, second and third trimesters.


Semiautomated assay for rapid determination of estrogens in late-pregnancy urine.


Computerizing medical records.

Computerizing medical records.


The biopsy-cucumber unit. A method to improve tissue orientation.


Deodorant vulvitis.

Deodorant vulvitis.


Internship, residency, and the coming demand for change.


The role of industry in national science policy. The people's welfare: health and medicine.


Negative feedback mechanisms in the architecture and function of the connective and cardiovascular tissues.


Plant pathology and world food problems.


And now--vein grafts.

And now--vein grafts.


Response of a cardiovascular surgeon to the editorial of Henry A. Zimmerman.


Reply to Dr. McGoon's remarks.

Reply to Dr. McGoon's remarks.


Natural history and surgical indications of ventricular septal defect.


Two x-ray signs helpful in the diagnosis of hypertrophic cardiomyopathy.


The significance of late-phased dart T wave in the electrocardiogram of children.


Fine structural lesions in the myocardium of a beer drinker with reversible heart failure.


A standard reference system for spatial vectorcardiography. comparison of the equilateral tetrahedron and the frank systems.


Arrhythmias following infusions of fatty acids.


Complete functional systolic obstruction of the right ventricular outflow in the tetralogy of Fallot.


Clinical recognition of atrial myxoma.

Clinical recognition of atrial myxoma.


Current status of diphenylhydantoin.

Current status of diphenylhydantoin.


A plea for evalutaing hemodynamic functions in dogs without the use of general anesthetic agents.


The atrial sound. Factors regulating its occurrence and timing.


Physical diagnosis of coronary artery disease.


Pityriasis rubra pilaris vs psoriasis.

Pityriasis rubra pilaris vs psoriasis.


Splenomegaly and blood pressure in an Orang Asli community in West Malaysia.


Liver disease associated with methotrexate treatment of psoriatic patients.


Effects of topical mechlorethamine on skin lesions of psoriasis.


Responses of mycosis fungoides to intensive external treatment with nitrogen mustard.


Dichlorophene (G-4) allergy.

Dichlorophene (G-4) allergy.


Interaction of phototoxic compounds with cells in tissue culture.


Postural hypotension and anhidrosis. The autonomic insufficiency syndrome.


Neomycin sensitivity in the normal (nonatopic) individual.


Percutaneous absorption following topical application of neomycin.


Media with pH indicators for the isolation of dermatophytes.


Lamellar ichthyosis--response to retinoic acid (tretinoin). A case report.


Incidence of skin cancer.

Incidence of skin cancer.


Polymorphic light eruption.

Polymorphic light eruption.


Photodermatitis from a sunscreening agent.


Lupus erythematosus and lupus vulgaris.

Lupus erythematosus and lupus vulgaris.


Dermatomyositis, calcinosis cutis, acanthosis nigricans, and exogenous hypercortisonism.


Sporotrichosis.

Sporotrichosis.


Primary macular atrophy.

Primary macular atrophy.


Perioral dermatitis.

Perioral dermatitis.


Granuloma annulare (nodular type on fingers).


The upper temperature limit of Cyanidium caldarium.


Fermentation of glucose by Chlorella.

Fermentation of glucose by Chlorella.


Toxicity of zinc, copper and lead to Chlorophyta from flowing waters.


Treatment of cardiogenic shock.

Treatment of cardiogenic shock.


Fragmentation of bovine chymotrypsinogen A and chymotrypsin A-alpha. Specific cleavage at arginine and methionine residues and separation of peptides, including B and C chains of chymotrypsin.


Peptide sequences and relative reactivity of the reactive sulfhydryl groups of rabbit muscle phosphorylase.


Cross-linking of collagen and elastin. Properties of lysyl oxidase.


Subunit structure of spinach leaf ribulose 1,5-diphosphate carboxylase.


Binding properties of dye-tagged polylysine complexed to deoxyribonucleic acid.


H nuclear magnetic resonance study of the copper(II)--carnosine complex in aqueous solution.


A description of two procedures which avoid the use of extreme pH conditions for the resolution of components isolated from chromatins prepared from pig cerebellar and pituitary nuclei.


Ester and ether-linked lipids in the mandibular canal of a porpoise (Phocoena phocoena). Occurrence of isovaleric acid in glycerolipids.


Analysis of macromolecule--ligand binding by determination of stepwise equilibrium constants.


Biosynthesis of 1,6-phenazinediol 5,10-dioxide (iodinin). Incorporation of shikimic acid.


Phosphorylation of mouse ascites tumor cell lysine-rich histone.


The selection of a small real-time computer for bio-medical application.


Temperature profile scanning--a more quantitative approach to thermography.


A new concept for a diagnostic centre.

A new concept for a diagnostic centre.


A tubular membrane oxygenator.

A tubular membrane oxygenator.


Differences between psychoanalysis and psychotherapy.


Countertransference, direct and indirect, in psychoanalysis.


A study of preadmission contacts at a veterans administration hospital.


The influence of social class and family size on adult psychiatric outpatients.


The uptake, distribution, and antitumor activity of 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea in the murine glioma.


DNA polymerase and DNA replication during lymphocyte transformation.


Growth stimulation of tissue culture cells derived from patients with neuroblastoma.


Influence of tumor-host differences at a single histocompatibility locus (H-1) on the antileukemic effect of 1,3-bis(2-chloroethyl)-1-nitrosourea (NSC 409962).


The induction of malignant melanomas in Syrian white hamster by neonatal exposure to urethan.


Amino acid induction and carbohydrate repression of dimethylnitrosamine demethylase in rat liver.


Mechanism of 3-methylcholanthrene-induced inhibition of dimethylnitrosamine demethylase in rat liver.


Phase I and preliminary phase II evaluation of adriamycin (NSC 123127).


The refractoriness of the skin of hairless mice to chemical carcinogenesis.


Susceptibility of guinea pigs to chemical carcinogens: 7,12-Dimethylbenz(a)anthracene and urethan.


Spontaneous development of mammary adenocarcinoma following prolonged immunosuppression in the dog.


Structure of high-energy molecules.

Structure of high-energy molecules.


Thermodynamics and biology.

Thermodynamics and biology.


Energetics of muscle.

Energetics of muscle.


A new imide from Butea frondosa.

A new imide from Butea frondosa.


Evidence for the origin of monoalkenes in cigarette smoke.


Miniature photometers for liquid chromatography.


Method for resolving and measuring overlapping chromatographic peaks by use of an on-line computer with limited storage capacity.


Diagnostic application of metabolic studies with isotopes.


Simultaneous multicolumn liquid-liquid and liquid-solid chromatography with a computerized readout system.


Two-dimensional thin-layer chromatography on two-layer plates of amino acids.


A bench-top, automated, high-resolution analyzer for ultraviolet absorbing constituents of body fluids.


Rapid separation of the components of nucleic acids and urine by high-resolution liquid chromatography.


Analysis of compounds of biological interest by electron spectroscopy.


Effect of a chemically defined diet on urinary excretion of minerals and aromatic compounds.


Preliminary results from high-resolution analyses of ultraviolet-absorbing and carbohydrate constituents in several pathologic body fluids.


Serum organic acids in sheep exposed to neutron-gamma irradiation, air blast, or both.


Ultraviolet-absorbing compounds in urine of normal newborns and young children.


Effect of dietary purine restriction, allupurinol, and oxipurinol on urinary excretion of ultraviolet-absorbing compounds.


Ultraviolet-absorbing components of urine from mentally retarded children. 3.


Gas-chromatographic identification of urinary carbohydrates isolated by anion-exchange chromatography.


Two-stage procedure for total pancreatectomy in the rat.


Intestinal absorption of sugars, water and sodium in alloxan diabetic rats.


Platelet phospholipids and their function in patients with juvenile diabetes and maturity onset diabetes.


Effect of pituitary ablation on diabetic  retinopathy studied by fluorescence angiography.


The glucose tolerance test and the response of intraocular pressure to topical corticosteroids.


Diabetes mellitus and pernicious anemia.


"Instant" blood sugar measurement using Dextrostix and a reflectance meter.


Estrogen monitoring in ovulation induction.


Vibratory sensitivity of the penis.

Vibratory sensitivity of the penis.


Timing of ovulation by a rapid luteinizing hormone assay.


Sperm motility and survival in relation to glucose concentration: an in vitro study.


Accumulation of phosphates in water.

Accumulation of phosphates in water.


Inhibition of growth and esterase activity in mouse fibroblast cell cultures by several organophosphorus insecticides.


Gas-liquid chromatographic determination of methyl parathion and metabolites.


Metabolism of carbofuran alfalfa residues in the dairy cow.


Volatilization of insecticides from various substrates.


Fate of benefin in soils, plants, artificial rumen fluid and the ruminant animal.


Reactions of certain trichloromethyl sulfenyl fungicides with low molecular weight thiols. In vitro studies with glutathione.


Reactions of certain trichloromethyl sulfenyl fungicides with low molecular weight thiols. In vivo studies with cells of Saccharomyces pastorianus.


Metabolism of propanil in soils.

Metabolism of propanil in soils.


Metabolism of 3-(p-bromophenyl)-1-methoxy-1-methylurea (metobromuron) by selected soil microorganisms.


Tangible reinforcers and child group therapy.


Chlorcholine chloride residues in grapes and their fate in winemaking.


Removal of gardona from fruit by commercial preparative methods.


1,3-bis(3,4-dichlorophenyl) triazene from propanil in soils.


Metabolic and residue studies with 2-(2,4,5-trichlorophenoxy)-ethyl 2,2-dichloropropionate (Erbon) herbicide in sheep.


Mother-infant relationship and weight gain in the first month of life.


Community child psychiatry: description of a training program and comments.


Characterization of thiamine in the raw peanut (Arachis hypogaea L.).


The function of ambiguity in child crises.


Effect of gamma radiation on polysaccharides and calcium distribution in carrot cell walls.


Transient school phobia in a leukemic child.


Residues in three peanut varieties grown in dieldrin treated soil.


Teacher-parent work in the home: an aspect of child guidance clinic services.


Persistence of azodrin residues on and in valencia oranges and in laboratory-processed citrus pulp cattle feed.


The absorption and metabolism of orally administered tritium labeled sodium stearyl fumarate in the rat and dog.


Improvement in the 2,3-diaminonaphthalene reagent for microfluorescent determination of selenium in biological materials.


Sensitized photolysis of heptachlor.

Sensitized photolysis of heptachlor.


The use of a play program by hospitalized children.


The effects of object loss on the body image of schizophrenic girls.


Imitation and echoing in young schizophrenic children.


Aflatoxins M1 and M2: preparation and purification.


Lipid and fatty acid composition of turkey liver, skin and depot tissue.


Tracking performance on a visual display apparently vibrating at one to ten Hertz.


A carboxypeptidase from germinated barley and its action on casein.


Guilty knowledge technique: application of signal detection measures.


Behavioral validation of several values from the Rokeach Value Scale as an index of honesty.


Conversion of ferric to ferrous iron in weight control dietaries.


Some observations in the management of 300 cases of acute myocardial infarction treated in the intensive coronary care unit.


Candida infection of urinary tract--an experimental study.


Sex life and psychiatric problems after myocardial infarction.


Immunoelectrophoresis of cerebrospinal fluid in neurological disorders.


Comparative study of hypoglycaemic treatment with insulin & tolubutamide in various types of neuroses.


Paraphyseal cyst (an autopsy report).

Paraphyseal cyst (an autopsy report).


Hydatiduria resulting from rupture of hydatid cyst of the kidney (a case report).


Determination of N-methylamino acids and their optical purity with an amino acid analyzer.


The rapid separation of nucleotides in cell extracts using high-pressure liquid chromatography.


Quantitative thin-layer chromatographic estimation of labeled dopamine and norepinephrine, their precursors and metabolites.


Detection and estimation of cyclamate by thin-layer chromatography.


Effects of self-monitoring on normal smoking behavior.


Income and outcome.

Income and outcome.


Failure of a moral enterprise: attitudes of the public toward mental illness.


Contextual constraint and schizophrenic language.


Autonomic reactivity to sensory stimulation in psychopathic, neurotic, and normal juvenile delinquents.


Treatment of "adolescent rebellion" by the reconstrual of stimuli.


Dimensions of manifest anxiety and their relationship to college achievement.


Anxiety and speech in the initial interview.


Treatment of a school phobia with implosive therapy.


Operant conditioning principles in the treatment of a selectively mute child.


Correlates of the psychotic reaction profile in an outpatient psychiatric sample.


Preferences for therapists and related expectancies.


Children's comprehension of between- and within-sentence syntactic structures.


Effects of discrimination, grammatical rules, and application of rules on the acquisition of grammatical concepts.


Relations between perceptual and syntactic control in oral reading.


Anxiety and intelligence in paired-associate learning.


Retroactive inhibition in meaningful verbal learning.


Observation of biological thick section with high voltage electron microscope.


Beryllium single crystal as substrate for high resolution electron microscopy.


Symposium; scanning electron microscope.


Symposium; protein synthesis and secretion.


Symposium; dynamic observation of metals.


Resolving power of the cathode lens.

Resolving power of the cathode lens.


Ultrastructural studies on arteriolar lesions in experimental hypertension.


Intracellular microcyst in gastric cancer cells.


Fine structures of interphase nuclei. II. The behavior of the chromatin during G1 period revealed by electron microscopic autoradiography.


Some new findings on the fine structure of the human photoreceptor cells.


The degradation of the photoreceptor outer segment within the pigment epithelial cell of rat retina.


The role of frustrative nonreward in instrumental escape conditioning.


Stimulus property mediation of paired-associates learning.


Influence of creativity on formation of subjective units.


Attention and perceptual learning.

Attention and perceptual learning.


Compensatory selection of magnesium sulfate by magnesium-depleted laboratory rats.


Preference and expectancy arousal: further evidence.


Urinary tract infection in children.

Urinary tract infection in children.


The aetiological pattern of jaundice in Gujarat.


Some observations on acute appendicitis.


The problem of diagnosis of prolonged cholestatic jaundice. A clinical review of 32 consecutive cases.


Osteo-articular complications of smallpox vaccination.


Hyperbilirubinaemia and jaundice.

Hyperbilirubinaemia and jaundice.


Magnesium.

Magnesium.


Hepatic amoebiasis in Kerala.

Hepatic amoebiasis in Kerala.


Phosphoramidate analogs of oligonucleotides.


Nucleosides from 3-deoxy-3-methylamino-D-ribofuranose.


The synthesis and rearrangement of 5-phenyl-7-methoxy-2,4,9-triketo-1,3-diazabicyclo[3.3.1]nonane.


Synthesis of a bicyclohydantoin.

Synthesis of a bicyclohydantoin.


The synthesis of pyrano- and furanopyrimidines from 3-haloprophyl- and 2-haloprophylbarbituric acids.


Alanylactinobolone. A basic hydrolysis product of the antibiotic aetinobolin.


Novel products from the oxidation of delta-5 steroids with potassium permanganate in pyridine.


Epoxidation of griseofulvin. A new reaction of the beta-methoxyenone system.


Evaluation of the Fisher autocytometer.

Evaluation of the Fisher autocytometer.


Qualitative analysis of hyaluronidase.

Qualitative analysis of hyaluronidase.


Improved filter-well apparatus for metabolic studies in vitro.


Automatic water mixing device.

Automatic water mixing device.


Aktograph for measuring locomotor movements of terrestrial insects.


Storage of freeze-dried cultures.

Storage of freeze-dried cultures.


Home hemodialysis: six years' experience.


Role of measles virus in skin lesions and Koplik's spots.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 48-1970.


Detection of venous thrombosis.

Detection of venous thrombosis.


Portal-vein hypoplasia with inferior mesenteric hypertension.


Disseminated intravascular coagulation.

Disseminated intravascular coagulation.


Low serum iron levels.

Low serum iron levels.


Red-cell 2,3-DPG level.

Red-cell 2,3-DPG level.


Diuretics and hyperuricemia.

Diuretics and hyperuricemia.


Mithramycin for Paget's disease of bone.


Urinary effects of calcitonin.

Urinary effects of calcitonin.


Neoplasia, hypercalcemia and mithramycin.


Diagnosis of myelomatosis.

Diagnosis of myelomatosis.


Diagnostic difficulties in hyperparathyroidism.


Alcohol-induced vitamin-B 6-responsive anemia.


Recurrent alcoholic sideroblastosis and hepatic hemosiderosis.


Mental illness in the general pediatric clinical population.


Creating a day hospital.

Creating a day hospital.


Maternal eating habits and fetal motility.


The use of guilt as a defense against anxiety.


Behind the dream screen.

Behind the dream screen.


The sin-neurotic complex: perspectives in religion and psychiatry.


Activity and reality.

Activity and reality.


The knees as fantasied genitals.

The knees as fantasied genitals.


Symptoms, insight, and behavior techniques in psychoanalytic psychotherapy.


Gender role determination. A critical review of the evidence from hermaphroditism.


Psychologic aspects of cigarette smoking in men. A clinical evaluation.


Influence of triiodothyronine on selective attention in man as measured by the visual averaged evoked potential.


An identical twin study of psychosocial factors in coronary heart disease in Sweden.


Tumoral calcinosis: a case report.

Tumoral calcinosis: a case report.


Dwarfism due to isolated growth hormone abnormalities.


The results of mitral valvotomy in patients over the age of fifty years.


The clinical syndrome and investigation of primary hyperparathyroidism at Groote Schuur Hospital.


A clinical study of the use of glifanan in labour.


Radiological detection of lactose malabsorption.


Pancreatic metastases from a malignant melanoma.


Methods and results of repeated insulin tests in patients with duodenal ulcer.


The gastrointestinal absorption and the excretion of H3-butylscopolamine (hyoscine butylbromide) in man.


Gastric evacuation and propulsive intestinal motility in experimental perforation peritonitis.


Influence of cholestyramine, bile salt, and cholesterol feeding on the lipid composition of hepatic bile in man.


Dose-response studies of gastric secretion of sodium, potassium, calcium, inorganic phosphorus, and magnesium after stimulation with pentagastrin.


Intravenous histamine test with fixed doses of histamine. Minimal dose of histamine for maximal acid secretion.


Effect of caerulein on pentagastrin-induced gastric acid secretion and peptic ulcers in cats.


The effect of metoclopramide on the gastroesophageal junctional zone and the distal esophagus in man.


A comparison between clinical state, macroscopic and microscopic appearances of rectal mucosa, and cytologic picture of mucosal exudate in ulcerative colitis.


Alcoholic hepatitis. A comparative study of two groups of patients with Mallory bodies with and without liver cell necrosis and neutrophilic infiltration in liver biopsies.


Chemical studies with amphotericin B. 3. The complete structure of the antibiotic.


Bromination of a Reformatsky adduct to 16-dehydropregnenolone acetate.


Crystal and molecular structure of grayanotoxin-I, C22H36O7.


Properties of a plant virus-induced RNA polymerase in particulate fractions of cucumbers infected with cucumber mosaic virus.


Electron microscopy of the replicative RNA from tobacco mosaic virus ana alfalfa mosaic virus: morphology and association of the double-stranded RNA.


Structure of tomato bushy stunt virus.

Structure of tomato bushy stunt virus.


Separation of the complementary strands and asymmetric in vivo transcription of coliphage phiII DNA.


Effect of glucosamine and of its derivatives on 35SO4-incorporation into dog aorta.


Critical flicker frequency series effect in epileptic and in neurotic patients. I. Differences in the daily level variation of the CFF series.


Calcitonin-effect of human blood plasma. I. Calcitonin-character of the calcium lowering plasma extract.


Calcitonin-effect of human blood-plasma. II. Plasma calcitonin activity in 18 subjects with normal calcium metabolism.


Pulmonary lipid metabolism in experimental fat embolism.


Effect of pineal body on thyroid-thymus correlations. 131-I uptake by rat thymus and salivary gland.


Permanent hypertension induced by ligation of one renal artery in the dog.


Rubeosis iridis.

Rubeosis iridis.


Studies on the thyroid gland of the nile teleost fish, Clarias lazera, C. and V.


Studies on absorption and topical distribution of oxytetracycline in healthy and experimentally infected animals.


A modified technique used for the clinical evaluation of testicular size in the bull.


Experiments on adoptive transfer of immunological tolerance and of immunity to Nippostrongylus brasiliensis in rats.


Muscular apparatus of the mitral valve in man and its involvement in left-sided cardiac hypertrophy.


Left ventricular abnormality with late mitral insufficiency and abnormal electrocardiogram.


Closure of ventricular septal defect and removal of pulmonary arterial band. Results in eight children.


Exophthalmos, stare, increase in intraocular pressure and systolic propulsion of the eyeballs due to congestive heart failure.


Experimental myocardial infarction. V. Reaction to impaired circumflex flow in the presence of established anterior myocardial infarction in intact conscious dogs.


Digitalization of the myocardium in the intact animal by direct coronary arterial drug administration. II. Relation of the rate of administration to myocardial concentration and toxicity.


Effects of digitoxin on the ultrastructural myocardial changes in the rat subjected to chronic exercise.


Automatic surveillance of heart rate and rhythm and T wave contour. The concept of automatic monitoring of large numbers of patients.


Complete heart block associated with acute myocardial infarction.


Pharmacodynamics and clinical use of cardiovascular drugs after cardiac surgery.


Clinically significant cardiac amyloidosis. Clinicopathologic findings in 15 patients.


Congestive heart failure in the newborn infant in the absence of primary cardiac disease.


Transmural myocardial necrosis after nonpenetrating cardiac trauma.


Aortic aneurysm with aortic insufficiency. Repair without prostheses.


Metastatic calcification of the myocardium.


Simulation of "left atrial rhythm" by right atrial pacing.


The radiographic appearance of the tracheobronchial tree as an indicator of visceral situs.


Coronary arteriography. A safe diagnostic procedure.


A classification for the cardiomyopathies.


Coumarin in pregnancy.

Coumarin in pregnancy.


Isolated tricuspid valvular stenosis.

Isolated tricuspid valvular stenosis.


Ascaris species in warthogs.

Ascaris species in warthogs.


Preliminary investigation of algal cellulose. I. X-ray intensity data.


Kinetics of acetylene and CN- reduction by the N2-fixing system of Rhizobium lupini.


Extracellular lactate dehydrogenase. Hormonal influences on the oviducal isoenzymes.


Accumulation of lycopene and inhibition of cyclic carotenoids in Mycobacterium in the presence of nicotine.


Oxidation of 7-azapteridines by mammalian xanthine oxidase.


Studies on the bilirubin sulphate conjugate excreted in human bile.


A study of the release of calcium from human blood platelets and its inhibition by metabolic inhibitors. N-ethylmaleimide and aspirin.


The effect of Ca2+ on the binding of steroids to human serum albumin.


Substrate specificity of phenethanolamine N-methyltransferase from rabbit adrenal.


Changes in oxygen consumption of isolated tissues after ouabain administration or substitution of choline for sodium in bathing media.


Preparative synthesis of saccharopine.

Preparative synthesis of saccharopine.


Activities of enzymes concerned with pyruvate, oxaloacetate, citrate, acetate and acetoacetate metabolism in placental cotyledons of sheep.


The metabolism of (+)-catechin to hydroxyphenylvaleric acids by the intestinal microflora.


Effect of reduction of disulfide bonds on uptake of intrinsic factor-cyanocobalamin complex by homogenates of guinea pig ileum.


A new derivative for the gas-liquid chromatography of picogram quantities of primary amines of the catecholamine series.


The development of L- and M-type aldolases in rat liver.


Studies in the biochemistry of skin. V. The particulate origin of adenylate kinase in the skin of neonatal rats.


Dietary regulation of glycolytic enzymes. X. The effect of oral, intramuscular and conjugated sex steroids on jejunal folate-metabolizing enzyme activities in normal and castrated male and female rats.


Inhibition of radiosulfate uptake in rat costal cartilage by lipophilic heterocyclic molecules.


The effect of auxin (2,4-dichlorophenoxyacetic acid) on the synthesis of cell wall polysaccharides in cultured sycamore cells.


Water weed control: the problem in Britain.


Selectivity of attack on cystine by hydroxyl radicals and chlorine atoms.


Reinvestigation of meiosis in the male goat, Capra hircus, Linn., with special reference to chiasma formation in the sex and autosomal bivalents.


Electron microscopy on mitotic and chromosomal aberrations induced by cholchicine, mercaptoethanol and nitrogen mustard.


The systemic incompatibility of medical practice and psychotherapy.


The neurological and psychiatric aspects of hypoglycemia.


Self-concept changes in training medical and nursing students.


Psychological needs of homosexual pedophiliacs.


Clinical neuropathological conference.

Clinical neuropathological conference.


"Continuing grief" as a method of psychotherapy following E.S.T. (A preliminary report).


Stress electrocardiography in the diagnosis of arrhythmias.


Potassium and arrhythmias.

Potassium and arrhythmias.


Anatomical basis of AV conduction disturbances.


Use of electrical pacemakers in the management of cardiac arrhythmias.


Could metals added to drinking water benefit the heart?


Commissioner on aging stresses complexity of problems of elderly.


The colon cancers which present an ambiguous picture.


Men who are young for their years.

Men who are young for their years.


New concepts in clinical gerontology.

New concepts in clinical gerontology.


Differential diagnosis of supraventricular and ventricular tachycardia.


When corticosteroid crystals confuse a diagnosis of gout.


The future of veterinary education in India.


Hematological constituents of blood of goats.


Residues of organochlorine insecticide in eggs and tissues of chickens fed low levels of the insecticide in ration.


Influence of cobalt supplementation on growth and digestibility of nutrient in rations containing urea.


Observations on the effects of preventive medication with various blendings of "terramycin" (Pfizer) on the incidence of white scours in calves.


Action of light upon the visual pigment rhodopsin. An experiment in biochemistry.


Safety enclosure for mercury filled McLeod Gauge.


The training of clinical chemists.

The training of clinical chemists.


Synthetic schistosomicides. Some antimonyl benzothiazole derivatives.


The use of frusemide in the management of portal hypertension. A preliminary report.


Studies on bacterial meningitis with special reference to some serum and cerebro-spinal fluid enzymes.


Traumatic diaphragmatic hernia due to closed injury (report of 4 cases).


Experimental studies of the physiological action of antimonials and Schistosoma mansoni worm antigen on the tracheo-bronchial tree.


Evaluation of new tools for the differential diagnosis of toxaemia of pregnancy.


Study of the portal and the cardiopulmonary haemodynamic interrelationship in schistosomal cases.


Unrecognized diabetes mellitus presenting with atypical neurological disorders.


Liver cell regeneration in prophylaxis and treatment of carbon tetrachloride hepatotoxicity.


Serum seromucoids and proteinogram in Sydenham's chorea in children.


Evaluation of the portal pressure and plasma protein alteration in the ascitic stage of bilharzial hepatic fibrosis.


Pneumoencephalography in geriatric psychiatric disorders.


On the effect of certain Annelids in the laboratory aquaria of some fresh water snails particularly the Molluscan vectors of bilharziasis.


Blood cholinesterases, hepatic renal and haemopoietic functions in children receiving repeated doses of "dipterex".


Some views on nasal allergy.

Some views on nasal allergy.


The differentiation of phospholipase A1 and A2 in rat and human nervous tissues.


Incorporation in vivo of radioactive leucine into neuronal and glial nuclear proteins of rat brain.


Properties and regional distribution of histidine decarboxylase in rat brain.


[U-14C]glucose metabolism of the perfused cat brain with blood flow disturbances.


Inhibition of bacterial and mammalian choline acetyltransferases by styrylpyridine analogues.


Pig brain glutaminase: purification and identification of different enzyme forms.


Thiamine pyrophosphatase activity in a fraction rich in golgi-like structures from crushed sciatic nerve of the cat.


Brain glutamate decarboxylase: changes in the developing rat brain.


Forebrain freezing rates and substrate levels in decapitated rat heads.


Effect of abdominal surgery on glucose tolerance, plasma levels of insulin and glycogenic amino acids.


Aspiration method of senile cataract.

Aspiration method of senile cataract.


Insulin-secreting capacity in newborn infants.


The growth and development of the American National Health System.


Occupational health--concerns and issues.


Management of depressed patients. A disucssion of some problems in psychotherapy.


Chronic serous otitis media.

Chronic serous otitis media.


Women's magazines vs public opinion.

Women's magazines vs public opinion.


Serous otitis media in children. Importance of prevention and early diagnosis.


Endocarditis. Clinical findings and management.


The common seborrheic wart as a test model for studying factors of controlled tissue growth.


The very busy doctor.

The very busy doctor.


The relevance of radiobiological research today.


The immunological treatment of malignant disease.


The white cell count in gram-negative shock.


Vascular abnormalities in congenital generalized elastolysis (cutis laxa): report of a case.


Racial significance in pyloric stenosis.


The direct utilization of unsaturated sugars in nucleoside synthesis. 3-deoxy-3-(6-chloro-2-methylthio-9-purinyl)-D-erythro-hex-1-enopyranose, a new and novel type of purine nucleoside.


Effect of radiation on the reproductive system of the Indian catfish, Heteropneustes fossilis Bloch. I. Annual cycle in the development of ovarian eggs.


An anatomical study of foetal blood vessels on the chorial surface of the human placenta.


A spatial model for the visualization of variations in the localization of sex chromatin.


RNA, hippocampus and learning: an autoradiographic study.


Quantitative structural analysis of human cancellous bone.


Morphological adaptation in developing vibrissae of rats.


Continuation of biological activity in excised small intestine of the adult white rat.


Grading of medical students and certification of pediatricians.


Symposium. Nutrition, Growth, and Mental Development. Eightieth annual meeting of the American Pediatric Society. Introductory remarks. Chairman's address.


The evaluation of human growth patterns.


Mental performance in school age children. Findings after recovery from early severe malnutrition.


Undernutrition and the developing brain. The relevance of animal models to the human problem.


Nutrition, growth, and mental development. Biological correlations.


Variation in palmar creases.

Variation in palmar creases.


Serum precipitins to Aspergillus fumigatus in cystic fibrosis.


Radiological case of the month.

Radiological case of the month.


Drug-induced localized sweating in neonates. Responses to exogenous and endogenous acetylcholine.


Diabetes mellitus and congenital rubella infection.


Cherubism.

Cherubism.


Hallermann-Streiff syndrome. Clinical and prognostic considerations.


G syndrome.

G syndrome.


Purpura fulminans. Complete recovery with intravenously administered heparin.


Möbius syndrome in twins.

Möbius syndrome in twins.


Hypoplasia of the abdominal aorta associated with the rubella syndrome.


Fat embolism in childhood. Review with report of a fatal case related to physical therapy in a child with dermatomyositis.


Thanatophoric dwarfism.

Thanatophoric dwarfism.


Mental retardation with absent fifth fingernail and terminal phalanx.


Indications of spatial relations among structures recognizing amino acids and Na+ at a transport receptor site.


The arrangement of proteins in the human erythrocyte membrane.


Localization of allantoinase in glyoxysomes of germinating castor beans.


Cytochrome b5 and CO-binding cytochromes in the Golgi membranes of mammalian livers.


Differential localization in thyroid microsomal subfractions of a mannosyltransferase, two N-acetylglucosaminyltransferases and a galactosyltransferase.


Evidence for extranucleolar control of RNA synthesis in the nucleolus.


Phosphorylation coupled to oxidation of thiol groups (GSH) by cytochrome c with disulfide (GSSG) as an essential catalyst. II. Demonstration of ATP formation from ADP and HPO4 2-.


Effect of epinephrine and its precursors on activity of proteolytic enzymes.


Complementary regions to tobacco mosaic virus RNA in host DNA.


Phosphorylation coupled to oxidation of thiol groups (GSH) by cytochrome c with disulfide (GSSG) as an essential catalyst. IV. Stability of intermediates and possible mechanism of reaction.


Protein chain initiation by methionyl-tRNA.


Dissociation and recombination of the subunits of human chorionic gonadotropin.


In vitro formation of intermolecular crosslinks in chick skin collagen. II. Kinetics.


Primary structure of two COOH-terminal hexapeptides from rabbit muscle aldolase: a difference in the structure of the alpha and beta subunits.


Enzymatic conversion of cholest-8(14)-en-3 beta, 15 alpha-diol and cholest-8(14)-en-3 beta, 15 beta-diol to cholesterol.


Substrate requirements of the Streptomyces albus G DD carboxypeptidase.


On the Streptomyces albus G DD carboxypeptidase mechanism of action of penicillin, vancomycin, and ristocetin.


Myosin-product complex and its effect on the steady-state rate of nucleoside triphosphate hydrolysis.


Determination of the number of regulatory and catalytic sites on aspartate transcarbamylase.


Catalysis of iodination by lactoperoxidase.


Kinetic evidence for a (4-amino-2-methyl-5-pyrimidinyl)methyl-enzyme intermediate in the thiaminase I reaction.


Change in conformation of the rabbit gamma G-immunoglobulin molecule with various chemical treatments.


Isozymes of rat liver mitochondrial malate dehydrogenase. Evidence for the existence of nonidentical subunits.


Dependence of sterol ester hydrolase activity on the position of ethylenic bond in cholesteryl cis-octadecenoates.


In vitro biosynthesis of membrane proteins in isolated mitochondria from Saccharomyces carlsbergensis.


Tritium-hydrogen exchange studies of polynucleotides. Double-stranded polyriboadenylic acid.


Polyploidy and mechanisms of karyotypic diversification in Amphibia.


Spontaneous aberrations in lampbrush chromosome XI from a specimen of Triturus vulgaris meridionalis (Amphibia, Urodela).


A complex familial translocation involving chromosomes 5, 9 and 13.


Diabetic retinopathy: study of the action of O-betahydroxyethyl-rutosides (HR) by retinal fluoresceinography.


Effects of the synalbumin insulin antagonist in vivo; its examination with a new method.


Observations on the effect of a ganglion blocking agent on responses to intravenous glucose infusion.


Circadian rhythm of glucose tolerance in laboratory mice.


Lactate dehydrogenase activity and isoenzyme patterns in skeletal muscle, fat, exocrine pancreas and isolated pancreatic islets of normal and obese-Hyperglycaemic mice.


Diabetes morbidity in a sociologically defined group of the general public (master bakers of a city). Investigation of carbohydrate metabolism.


Relevance of physiological and psychological criteria to man-machine systems: the present state of the art.


Genetic control of a multienzyme complex: subunit structures of mutationally altered forms.


Properties of bacteriophage T4 mutants defective in DNA polymerase.


Genetic analysis of the aberrant behavior of an X-chromosome duplication in the germ line of Drosophila melanogaster males.


The genetic basis of natural variation. X. Recurrence of cve polygenes.


Genetic analysis of azaguanine resistance in an established mouse cell line.


The cytoplasmically-inherited "sex-ratio" condition in natural and experimental populations of Drosophila bifasciata.


Viability mutations induced by ethyl methanesulfonate in Drosophila melanogaster.


Quantitative genetics of fertility. I. Lifetime egg production of Drosophila melanogaster--theoretical.


Experience of acute coronary insuffciency in a coronary care unit.


Rheumatic fever: a ten year review of patients admitted to an Irish children's hospital. Diagnosis.


The biological availability of iron from wheat bread.


Transient global amnesia. A report of eleven cases, including five of amnesia at the seaside.


An analysis of O2 debt contracted in submaximal exercise.


Effects on maximal oxygen intake of acute changes in altitude in a deep mine.


Individual differences in critical temperatures among rats at various ages.


Blood glucose in high-altitude natives and during acclimatization to altitude.


Effect of systemic and pulmonary venous hypertension on pleural and pericardial fluid accumulation.


Energy cost of "hard work".

Energy cost of "hard work".


Coronary collateral flow studies during variable aortic root pressure waveforms.


Solubility of nitrous oxide in biologic fluid and myocardium.


Sensitivity to central and peripheral thermal stimulation in man.