An analysis of the spontaneous activity of lateral geniculate neurons and of optic tract fibers in free moving cats.


New derivatives of alpha-ethyl-alpha-allophanyl-gamma-valerolactone.


Sleep and environmental temperature.

Sleep and environmental temperature.


Amino acid requirement for the growth-hormone stimulation of incorporation of precursors into protein and nucleic acids of liver slices. 1. Incorporation of [(14)C]leucine into protein in rat liver slices, incubated in vitro, increased as the concentration of unlabelled amino acids in the incubation medium was raised. A plateau of incorporation was reached when the amino acid concentration was 6 times that present in rat plasma. Labelling of RNA by [(3)H]orotic acid was not stimulated by increased amino acid concentration in the incubation medium. 2. When amino acids were absent from the medium, or present at the normal plasma concentrations, no effect of added growth hormone on labelling of protein or RNA by precursor was observed. 3. When amino acids were present in the medium at 6 times the normal plasma concentrations addition of growth hormone stimulated incorporation of the appropriate labelled precursor into protein of liver slices from normal rats by 31%, and into RNA by 22%. A significant effect was seen at a hormone concentration as low as 10ng/ml. 4. Under the same conditions addition of growth hormone also stimulated protein labelling in liver slices from hypophysectomized rats. Tissue from hypophysectomized rats previously treated with growth hormone did not respond to growth hormone in vitro. 5. No effect of the hormone on the rate or extent of uptake of radioactive precursors into acid-soluble pools was found. 6. Cycloheximide completely abolished the hormone-induced increment in labelling of both RNA and protein. 7. It was concluded that, in the presence of an abundant amino acid supply, growth hormone can stimulate the synthesis of protein in rat liver slices by a mechanism that is more sensitive to cycloheximide than is the basal protein synthesis. The stimulation of RNA labelling observed in the presence of growth hormone may be a secondary consequence of the hormonal effect on protein synthesis. 8. The mechanism of action of growth hormone on liver protein synthesis in vitro was concluded to be similar to its mechanism of action in vivo.


Incorporation of [3H] uridine into the ribonucleic acid of rat uterus during pseudopregnancy and in the presence of I.C.I. 46474 [trans-1-(p-beta-dimethylaminoethoxyphenyl)-1,2-diphenylbut-1-ene]. 1. The incorporation of [(3)H]uridine into RNA of rat uterine tissue has been measured during pseudopregnancy and in rats receiving different doses of an anti-implantation compound [trans-1-(p-beta-dimethylaminoethoxyphenyl)-1,2-diphenylbut- 1-ene, I.C.I. 46474]. 2. In the uterus of the pseudopregnant rat uridine incorporation into RNA increased markedly on day 3 of pseudopregnancy, remained high until day 5 and decreased sharply by day 6, rising again by day 9. 3. This pattern of change was similar to, but not identical with, that previously found in the pregnant rat. 4. Rats receiving I.C.I. 46474 at a dose concentration below that preventing implantation showed a pattern of RNA synthesis similar to that found in untreated control rats. 5. Rats treated with doses of I.C.I. 46474 sufficient to inhibit implantation revealed a totally different pattern of incorporation of [(3)H]uridine into uterine RNA. 6. The results are discussed in terms of previous findings with the normally pregnant rat. It is concluded that the increasing uterine RNA synthesis found on day 3 in the pregnant rat is an important requirement for the occurrence of the subsequent implantation.


Studies on a testosterone glucuronyltransferase from the cytosol fraction of human liver. An enzyme that conjugates the 17beta-hydroxyl group of testosterone was found in the cytosol fraction of human liver. The same enzyme preparation also conjugates the 16alpha-hydroxyl group of oestriol. The enzymic activity could not be sedimented by centrifuging the cytosol fraction at 158000g(av.) for 120min. The testosterone-conjugating as well as the oestriol-conjugating activities were found in the precipitate obtained after 30% saturation of the cytosol fraction with ammonium sulphate. Filtration of the precipitate through Sephadex G-200 enriched the testosterone-conjugating enzyme 50-fold and the oestriol-conjugating enzyme 100-fold. No separation of the two activities was achieved. With labelled testosterone the product of the reaction, testosterone 17beta-glucuronide, was identified by paper chromatography and by crystallization to constant specific radioactivity. Testosterone 17beta-glucuronyltransferase was active between pH7.0 and 8.6 in tris-HCl and tris-maleate buffers. The apparent K(m) values for testosterone and UDP-glucuronic acid were 6.4 and 25mum respectively. The enzyme was active between 37 and 45 degrees C; the activation energy was calculated to be 5kcal/mol. Oestriol did not influence the glucuronidation of testosterone. Controlled heating as well as alternate freezing and thawing of the purified enzyme preparation led to an inactivation of both testosterone-conjugating and oestriol-conjugating activities at similar rates. Testosterone and oestriol, when incubated together, gave a reaction rate that was approximately equal to the sum of the rates when the two substrates were incubated separately. The present findings suggest that testosterone and oestriol are conjugated by two separate enzymes.


Effect of actinomycin D, cycloheximide, and acute blood loss of ferritin synthesis in rat liver. 1. The mechanism of the stimulation of ferritin synthesis by iron in vivo has been studied in rat liver. Ferritin synthesis and turnover was measured by [(14)C]leucine incorporation. 2. Actinomycin D had no inhibitory effect, after administration of iron, on [(14)C]leucine incorporation into ferritin but appeared to augment the effect of iron on ferritin synthesis. 3. Cycloheximide completely abolished the stimulation by iron of [(14)C]leucine into ferritin and was subsequently utilized to show that iron acts in vivo by translational induction of apoferritin synthesis, rather than by stabilization of apoferritin or its precursors. 4. This conclusion was confirmed by showing that 2 days after acute bleeding, when iron was in the process of being removed from hepatic ferritin stores, ferritin synthesis was decreased whereas breakdown rates were unchanged.


Studies on bound trans-4-hydroxy-L-proline in sandal (Santalum album L.). 1. trans-4-Hydroxy-l-proline was found to occur in the bound state in the leaves of sandal (Santalum album L.), in which large amounts of free cis-4-hydroxy-l-proline are also present. 2. Bound trans-4-hydroxy-l-proline was present, associated mainly with a ;wall' fraction and a ;soluble' fraction roughly in equal proportions. 3. Bound proline is present only in small amounts in the ;soluble' fraction but is mostly associated with the ;wall' fraction and the other sedimented fractions. 4. In the free amino acid fraction more than 98% of the hydroxyproline had the cis-configuration, whereas in the ;wall' and ;soluble' fractions more than 90% of the bound hydroxyproline was in the trans-configuration. 5. Various extraction procedures indicated heterogeneity of the hydroxyproline-containing components. Hot 5% (w/v) trichloroacetic acid extracts about 25% of hydroxyproline and m-NaOH extracts an additional 25%. 6. Incorporation of [(14)C]proline into the bound hydroxyproline was demonstrated. The hydroxyproline component of the ;soluble' fraction does not appear to be the precursor of that of the ;wall' fraction.


Conversion of 5-(1,2-epoxy-2,6,6-trimethylcyclohexyl)-3-methylpenta-cis-2-trans-4-dienoic acid into abscisic acid in plants. (+/-)-5-(1,2-Epoxy-2,6,6-trimethylcyclohexyl) -3-methyl[2-(14)C]penta-cis-2-trans-4-dienoic acid is converted into abscisic acid by tomato fruit in 1.8% yield (or 3.6% of one enantiomer if only one is utilized) and 15% of the abscisic acid is derived from the precursor. The 2-trans-isomer is not converted. The amounts of [2-(3)H]mevalonate incorporated into abscisic acid have shown that the 40-times higher concentration of (+)-abscisic acid in wilted wheat leaves in comparison with unwilted ones reported by Wright & Hiron (1969) arises by synthesis. The conversion of (+/-)-5-(1,2-epoxy-2,6,6-trimethylcyclohexyl) -3-methyl-[2-(14)C]penta-cis-2-trans-4-dienoic acid into abscisic acid by wheat leaves is also affected in the same way by wilting and it is concluded from this that the epoxide or a closely related compound derived from it is on the biosynthetic pathway leading to abscisic acid. The oxygen of the epoxy group was shown, by (18)O-labelling, to become the oxygen of the tertiary hydroxyl group of abscisic acid.


Properties and substrate specificities of the phenylalanyl-transfer-ribonucleic acid synthetases of Aesculus species. 1. Phenylalanyl-tRNA synthetases have been partially purified from cotyledons of seeds of Aesculus californica, which contains 2-amino-4-methylhex-4-enoic acid, and from four other species of Aesculus that do not contain this amino acid. The A. californica preparation was free from other aminoacyl-tRNA synthetases, and the contaminating synthetase activity in preparations from A. hippocastanum was decreased to acceptable limits by conducting assays of pyrophosphate exchange activity in 0.5m-potassium chloride. 2. The phenylalanyl-tRNA synthetase from each species activated 2-amino-4-methylhex-4-enoic acid with K(m) 30-40 times that for phenylalanine. The maximum velocity for 2-amino-4-methylhex-4-enoic acid was only 30% of that for phenylalanine with the A. californica enzyme, but the maximum velocities for the two substrates were identical for the other four species. 3. 2-Amino-4-methylhex-4-enoic acid was not found in the protein of A. californica, so discrimination against this amino acid probably occurs in the step of transfer to tRNA, though subcellular localization, or subsequent steps of protein synthesis could be involved. 4. Crotylglycine, methallylglycine, ethallylglycine, 2-aminohex-4,5-dienoic acid, 2-amino-5-methylhex-4-enoic acid, 2-amino-4-methylhex-4-enoic acid, beta-(thien-2-yl)alanine, beta-(pyrazol-1-yl)alanine, phenylserine and m-fluorophenylalanine were substrates for pyrophosphate exchange catalysed by the phenylalanyl-tRNA synthetases of A. californica or A. hippocastanum. Allylglycine, phenylglycine and 2-amino-4-phenylbutyric acid were inactive.


Properties and substrate specificity of the leucyl-, the threonyl- and the valyl-transfer-ribonucleic acid synthetases from Aesculus species. 1. Leucyl- and threonyl-tRNA synthetases were partially purified up to 100-fold and 30-fold respectively from cotyledons of Aesculus hippocastanum and were largely separated from the other aminoacyl-tRNA synthetases. Valyl-tRNA synthetase was purified 25-fold from cotyledons of Aesculus californica. 2. Some properties are reported for the three enzymes when assayed by the [(32)P]pyrophosphate-ATP exchange technique. 3. beta-(Methylenecyclopropyl)alanine, isoleucine, azaleucine, norleucine and gamma-hydroxynorvaline acted as alternative substrates for the leucyl-tRNA synthetase; the enzyme's affinity for beta-(methylenecyclopropyl)-alanine and for isoleucine was about 80-fold less than that exhibited for leucine. 4. alpha-Cyclopropylglycine and alpha-cyclobutylglycine acted as alternative substrates for the valyl-tRNA synthetase.


Immunochemical studies with soluble and mitochondrial pyruvate carboxylase activities from rat tissues. 1. Pyruvate carboxylase (EC 6.4.1.1), purified from rat liver mitochondria to a specific activity of 14 units/mg, was used for the preparation of antibodies in rabbits. 2. Tissue distribution studies showed that pyruvate carboxylase was present in all rat tissues that were tested, with considerable activities both in gluconeogenic tissues such as liver and kidney and in tissues with high rates of lipogenesis such as white adipose tissue, brown adipose tissue, adrenal gland and lactating mammary gland. 3. Immunochemical titration experiments with the specific antibodies showed no differences between the inactivation of pyruvate carboxylase from mitochondrial or soluble fractions of liver, kidney, mammary gland, brown adipose tissue or white adipose tissue. 4. The antibodies were relatively less effective in reactions against pyruvate carboxylase from sheep liver than against the enzyme from rat tissues. 5. Pyruvate carboxylase antibodies did not inactivate either propionyl-CoA carboxylase or acetyl-CoA carboxylase from rat liver. 6. It is concluded that pyruvate carboxylase in lipogenic tissues is similar antigenically to the enzyme in gluconeogenic tissues and that the soluble activities of pyruvate carboxylase detected in many rat tissues do not represent discrete enzymes but are the result of mitochondrial damage during tissue homogenization.


Effects of oestradiol-17-beta and progesterone on total and nuclear-protein synthesis in epithelial and stromal tissues of the mouse uterus, and of progesterone on the ability of these tissues to bind oestradiol-17-beta. 1. A method is described for separating uterine epithelium that is 80% pure and connective-tissue stroma that is 60% pure. This was used to study the effects of steroid hormones on total and nuclear-protein synthesis in these tissues. 2. Oestradiol-17beta given alone produces mitoses in the epithelium but not in the stroma. It stimulated incorporation in vitro of [(14)C]lysine into total protein, histones and acidic nuclear proteins to a greater extent in epithelium than stroma. Incorporation into acidic nuclear proteins was most markedly stimulated, reaching four to six times the normal value 4h after treatment, and then declining rapidly. This peak was only seen in epithelial preparations. 3. After pretreatment with progesterone, oestradiol-17beta has the reverse effect, producing mitoses only in stroma. Progesterone alone had no effect on the amounts or rates of incorporation of [(14)C]lysine into stromal nuclear proteins, but changes after oestradiol-17beta treatment were similar to those seen in epithelium with oestradiol-17beta alone. In the epithelium, progesterone alone depressed incorporation into histones and acidic nuclear proteins, but did not abolish the subsequent response to oestradiol-17beta. With this treatment there was a rapid, large and transient increase in incorporation into epithelial total protein not seen with oestradiol-17beta alone. 4. Progesterone had no qualitative effect on the distribution of specific oestrogen-binding proteins, as judged by sucrose-density-gradient centrifugation. However, progesterone treatment increased the uptake in vivo of [6,7-(3)H]oestradiol-17beta by stroma, and it is possible that this is important although the differences were not apparent after labelling in vitro.


A method for the analysis of phosphate and calcium in small samples of plasma by atomic absorption spectrophotometry.


Creatine regulation in the embryo and growing chick. 1. The absence of creatine was demonstrated enzymically in the hen's-egg yolk and in the albumin contrary to former reports. 2. A comparison of the results obtained by enzymic and colorimetric methods to measure creatine is presented. 3. Creatine phosphate was not detected in the yolk extracts. 4. The content of free arginine enzymically assayed was 15.7mumol in the yolk and 3.38mumol in the albumin. Arginine amounts to practically all of the guanidine compounds in the yolk and one-half of those in the albumin. 5. No glycine amidinotransferase activity was found in the egg-yolk homogenates. 6. The heart of the chick embryo does not receive creatine from the egg and the creatine kinase activity present in this organ starting from the 27th hour of incubation suggests that the enzyme is a constitutive one working probably as an adenosine triphosphatase in a way similar to the kinase isolated from rabbit skeletal muscle. 7. Liver glycine amidinotransferase activity appeared clearly after day 5 of incubation. The specific activity reached a maximum at day 12 and then declined; however, the activity per total mass of liver increased steadily during all the prenatal period. Concomitantly with this steady increase a rise in the creatine content of the whole embryo was observed. An analogous increasing relationship between total liver amidinotransferase activity and liver creatine content was also detected during the postnatal period. 8. Repression of amidinotransferase by creatine cannot be accepted as occurring under physiological conditions since an inverse relationship between the two parameters was not observed. 9. Repression of liver amidinotransferase is observed only when pharmacological concentrations of the exogenous creatine are present in the chick liver.


Free and membrane-bound polyribosomes in normal and Rauscher-virus-infected mouse spleen cells. 1. Free and membrane-bound polyribosomes and ribosomal monomers were isolated from normal and Rauscher-virus-infected mouse spleens by means of discontinuous sucrose density gradients. 2. The addition of ribonuclease inhibitor from rat liver was essential to protect these polyribosomes from degradation. To separate the smooth and rough membranes from ribosomal monomers an additional centrifugation step through a continuous sucrose density gradient was necessary. 3. After infection a marked increase in rRNA from both membrane-bound and free polyribosomes was observed. Treatment of the membrane-bound polyribosomes with sodium deoxycholate yielded only 80S particles even when ribonuclease inhibitor was added. 4. A striking feature of the infected spleen was the occurrence of large polyribosomes. Up to 40 monomers per polyribosome could be counted on electron micrographs.


The effect of prostatic metabolites of testosterone and other substances on the isolated deoxyribonucleic acid polymerase of the canine prostate.


Formation of hydrogen sulphates as a means of separating alcoholic from non-alcoholic steroids.


Mikulicz's recurrent oral aphthae: the effect of anti-lymphocyte serum upon the in vitro cytotoxicity of lymphocytes from patients for oral epithelial cells.


Suppression of antibodies to nucleic acids with polyinosinic-polycytidylic acid and cyclophosphamide in murine lupus.


Cerebrospinal fluid beta-glucuronidase activity in rabbits with experimental allergic encephalomyelitis.


Studies on antilymphocyte antibody in the chicken. I. Effect of rabbit antithymus and antibursa globulin on chicken embryos.


Production of 19S and 7S antibodies by cancer patients.


An immunological basis for the anaemia of acute Salmonella gallinarum infection of chicken. I. Haematological changes and their association with in vivo modification of the erythrocytes.


Inhibitors of the fibrinolytic enzyme system in renal disease.


An immunological basis for the anaemia of acute Salmonella gallinarum infection of chicken. II. The relationship of the immune response to the development of the haemolytic anaemia.


The value of plasma renin concentration per se, and in relation to plasma and extracellular fluid volume in diagnosis and prognosis of human renovascular hypertension.


Interrelation between restitution time-constant and alternating myocardial contractility in dogs.


Protein turnover in skeletal muscle. II. The effect of starvation and a protein-free diet on the synthesis and catabolism of skeletal muscle proteins in comparison to liver.


Protein turnover in skeletal muscle. I. The measurement of rates of synthesis and catabolism of skeletal muscle protein using (14C)Na2CO3 to label protein.


The relative importance of urinary pH and urinary content of citrate, magnesium and calcium in the production of nephro calcinosis by diet and acetazolamide in the rat.


Factors determining the ventilatory response to carbon dioxide in chronic obstructive airways disease.


The effects of prolonged administration of vasopressin and oxytocin on renin, aldosterone and sodium balance in normal man.


Exercise tolerance breathing a low density gas mixture, 35 per cent oxygen and air in patients with chronic obstructive bronchitis.


The effect of hypoglycaemia on oral glucose tolerance in normal subjects and patients with pituitary and adrenal disorders.


Effect of vasopressin on the renal excretion of phosphate in man.


Prolonged neonatal hyperbilirubinemia and pregnane-3(alpha),20(beta)-diol in maternal milk.


A case of agenesis of the dorsal pancreas with interventricular septal defect in an infant.


A further observation on the metabolism of calcium and inorganic phosphate in vitamin-D-resistant rickets, with reference to the effects of osteotomy and vitamin D.


Group psychotherapy with suicidal persons: a comparison with standard group methods.


Haptenated bacteriophage in the assay of antibody quantity and affinity: maturation of an immune response.


The psycholytic treatment of a childhood schizophrenic girl.


Education as a function of mental health.


A social approach to an understanding of schizophrenic-like reactions.


Trance and possession states.

Trance and possession states.


Criminosynthesis.

Criminosynthesis.


Klinefelter's syndrome in children.

Klinefelter's syndrome in children.


On the reliability of the Graham-Rosenblith behaviour test for neonates.


School phobia and self-evaluation.

School phobia and self-evaluation.


The world test: developmental aspects of a play technique.


A modern medicolegal investigative system within the framework of a State Division of Health--experience in Utah.


Disguised handwriting. A statistical survey of how handwriting is most frequently disguised.


Histochemical changes as evidence of the antemortem origin of skin burns.


The use of radioactive iodine 125 for dental identification in mass disasters.


The toxicology of Abrus precatorius linnaeus.


Medicolegal investigation of a bomb explosion in an automobile. Chronological account of the explosion in Bel Air, Md., March 9, 1970.


Prolonged survival with retention of bullet in orbit, lung, or spleen. Report of three cases. The identification of bullets. The relationship between trauma and malignancy.


Disseminated magnesium and aluminum silicate associated with paregoric addiction.


The identification of lysergic acid amide in baby Hawaiian woodrose by mass spectrometry.


A spectrophotofluorometric method for the determination of amphetamine.


Determination of warfarin in liver tissue by gas chromatography.


Entomophthora conidia in the air-spora.

Entomophthora conidia in the air-spora.


The effect of nickel on a marine bacterium, Arthrobacter marinus sp.nov.


Persistance in mice of the L-phase of three streptococcal strains adapted to physiological osmotic conditions.


Lactobacillus jensenii sp.nov., a new representative of the subgenus Thermobacterium.


Re-establishment of Entodinium caudatum, cultured in vitro, in the rumen of a defaunated sheep.


The walls of the extremely halophilic cocci: gram-positive bacteria lacking muramic acid.


The 75th anniversary of the National Medical Association.


Through stormy waters under full sail. Address of the retiring president.


The black physician in the 1970s.

The black physician in the 1970s.


Pseudo-tumor cerebri.

Pseudo-tumor cerebri.


Further studies of respiratory bellows dynamics in sarcoidosis.


Thyroid surgery at Hubbard Hospital, 1946-1969.


Dentigerous cyst in maxillary sinus.

Dentigerous cyst in maxillary sinus.


Howard University Mississippi Project.

Howard University Mississippi Project.


A position paper on admissions to the University of Minnesota Medical School.


Black psychiatry one year after Miami.

Black psychiatry one year after Miami.


Ultrasonic fetal cephalometry during the second trimester of pregnancy.


The measurement of extracellular fluid volume in pregnancy with inulin.


The duration of induced labour.

The duration of induced labour.


A safe alternative to Caesarean section?


Acid-base relationships across the sheep placenta.


Comparison between plasma oestriol and urinary oestriol levels in pregnancy.


Maternal death from systemic sclerosis. (Report of a case of renal scleroderma masquerading as pre-eclamptic toxaemia).


Syntocinon induced convulsion.

Syntocinon induced convulsion.


Dystocia due to congenital polycystic kidneys.


The relation of cervical mucus proteins to sperm penetrability.


The effects of schistosomiasis on the cervix uteri in the African female.


The role of fetal death in the process of therapeutic abortion induced by intra-amniotic injection of hypertonic saline.


Incidental pathology at vaginal hysterectomy for genital prolapse.


Perforation of bicornuate uterus by intrauterine contraceptive device.


Finance of group practice from a non-health centre point of view.


Placenta percreta with penetration of the bladder.


Group practice: finance from a health centre point of view.


A symposium on group practice held at the Royal College of General Practitioners 4 December 1969.


Administration in group practice.

Administration in group practice.


Group practice: records and confidential record handling.


Group practice: computers and record keeping.


Group practice: equipment.

Group practice: equipment.


Design of premises for group practice.

Design of premises for group practice.


Scope of ancillary (paramedical) staff in group practice. A preliminary report.


Motivation for setting up group practice and employing staff.


Communication problems.

Communication problems.


Diagnostic facilities in group practice.


Group practice: teaching and research practices.


Operational problems in group practice.

Operational problems in group practice.


Group practice: setting up of a centre.

Group practice: setting up of a centre.


Medical manpower for occupational health in the tropics--professional staff: needs and training.


Medical manpower for occupational health in the tropics. 2. The auxiliary--his role and training.


Medical manpower for occupational health in the tropics. 3. The service approach to occupational health in a developing country.


Review of the opportunities for co-operative work. 1. Training courses and interchange of staff.


Review of the opportunities for co-operative work. 2. Information and advisory services.


Review of the opportunities for co-operative work. 3. Research--applied and operational.


Health problems of industrial progress in developing countries. Conclusions of the symposium.


When is cannabis resin?

When is cannabis resin?


Medico-legalism.

Medico-legalism.


Separation and identification of some organophosphorus pesticides from liver by two-dimensional TLC.


The 3 special hospitals in England and patients with dangerous, violent or criminal propensities. 3. Restricted patients.


Sudden death in the gay world.

Sudden death in the gay world.


Growth curves and nucleic acids content of mycelial and yeast-like forms of Paracoccidioides brasiliensis.


Biochemical and genetical studies on host-parasite relationship: studies on the hydrolytic enzymes of two mutants of Fusarium vasinfectum ATK.


Examination of several selected fungi by scanning electron microscope.


Toxicity of fungus, Microsporom cookei, to experimental animals.


Preliminary study on the influence of fungal metabolites on germination of barley grains.


Fungi associated with strawberry root rot in Illinois.


Trichophyton simii infection in man and animals.


The nature of the secondary conidia of Sporothrix schenckii.


Pharyngitis.

Pharyngitis.


Nasal polypi and the deviated nasal septum.


Snuffles in childhood.

Snuffles in childhood.


Mite asthma. Cause and management.

Mite asthma. Cause and management.


Lead poisoning presenting as infectious disease.


Aerophagy and flatulence.

Aerophagy and flatulence.


Ano-rectal and perineal disorders of pregnancy and the puerperium.


Cephalexin--a new oral antibiotic.

Cephalexin--a new oral antibiotic.


Care of the elderly in the community. A public health approach.


The use of 'Septrin' in the treatment of upper respiratory-tract infections.


Thoughts at random. A running commentary.


Group practice in the Far East.

Group practice in the Far East.


Haemophilia.

Haemophilia.


Preliminary studies of the venoms of some vermivorous conidae.


Some pharmacological characteristics of the venom of the alphabet cone, Conus spurius atlanticus.


The effect of crude holothurin on leucocyte phagocytosis.


Milking of arthropods.

Milking of arthropods.


Binding of iodinated erabutoxin b, a sea snake toxin, to the endplates of the mouse diaphragm.


Techniques for experimental brown spider envenomation.


Incidence of ciguatera in barracuda from the west coast of Florida.


Late results in re-treatment of patients with pulmonary tuberculosis.


Local treatment of aspergilloma of the lung with a paste containing nystatin or amphotericin B.


Cardiac manifestations in PAS hypersensitivity.


The Heaf test.

The Heaf test.


Basic science teaching in veterinary medicine: an adventure in heresy.


Changes in lactate dehydrogenase(LDH) isoenzymes associated with retinal degeneration of ewes affected with bright blindness.


The isolation of a strain of Trypanosoma vivax resistant against Novidium from cattle in Northern Nigeria.


Morphological study of the lateral nasal gland of the one-humped camel.


Histochemical study of cell components and fatty yolk formation in the developing oocyte of Rana pipiens.


A radiological approach to early tooth morphology in small mammals.


Differentiation of the dorsal mesentery in man.


Morphologic aspects of the influence of experimental microembolism on cerebral microcirculation.


The nucleus supragenualis, presumptive superior salivatory nucleus.


A quantitative study of the cross-sectional areas of the transverse and sigmoid dural venous sinuses in human foetuses and adults, with a note on changes in form which these sinuses undergo before birth.


Leuko- and oncogenesis in the light of studies on the metabolism of magnesium and its turnover in biocenosis.


Leuko- and oncogenesis in the light of studies on the metabolism of magnesium and its utrnover in biocenosis.


On the intra-individual variability of betanin excretion with urine.


Haptoglobin (Hp) levels in the blood serum of patients suffereing from leukemia.


Observations on the Xe human globulin group system.


Blood enzymes in Duchene's progressive muscular dystrophy and their correlation with the clinical and histological pictures.


Porolite silicosis.

Porolite silicosis.


An unusual case of periosteal deformity of the cranium and some of the skeletal bones. (Periostoso-osteosclerosis).


A projection from the nucleus Z to the ventral nuclear complex of the thalamus in the cat.


Visually guided behaviour after total neonatal removal or the neocortex in the cat.


An aquatic swim-mill.

An aquatic swim-mill.


The stimulating and inhibitory effect of carbamoylcholine on decamethonium uptake in mouse kidney slices.


Effect of training with eccentric muscle contractions on human skeletal muscle metabolites.


Energy metabolism during isometric exercise at different temperature of M. quadriceps femoris in man.


Ventilatory response to CO2 in hyperbaric environments.


Microelectrode recording from the facial nucleus in the cat.


Regulation of depth and rate of breathing in cat and man.


Increased activity in vagal cardiac afferents correlated to the appearance of reflex bradycardia during severe hemorrhage in cats.


Nervous influence on the pulmonary blood volume.


Microneurographic analysis of the fibre spectrum of human sensory nerve fascicles.


Excitatory and inhibitory propriospinal pathways from lumbo-sacral to cervical segments in the cat.


Ethanol, electrical stimulation, and net movements of sodium and potassium in rat brain tissue in vitro.


On the dynamic properties of the middle ear reflexes.


Dynamics of fluid transfer between the intra- and extravascular compartments during exercise.


Physiological interrelations during intermittent and continuous exercise with the same average power output in man.


Hyperpolarizing actions of noradrenaline in spinal motoneurones.


Autonomic innervation of the smooth muscle sphincter in the swimbladder of a fish (Gadus morhua).


Vasopressin content in the neurohypophysis of rats during overhydration and rehydration.


The effect of slight hypothermia on hepatic excretory function in cats.


Changes in spinal excitability during an auditory vertex response in man.


Metabolism of subcutaneously injected 14C-histamine in myxine glutinosa.


Thalamo-cortical rhythmicity during reversible visual deafferentation.


Effects of prostaglandin E1 in canine subcutaneous adipose tissue in situ.


Reduction in pulmonary blood volume after a blood loss.


Influence of plasma hypertonicity on blood viscosity studied in vitro and in an isolated vascular bed.


Efflux of prostaglandins in lymph from scalded tissues.


The permeability of the blood-brain barrier to lactate and pyruvate.


A double isotope method for determination of the miscible inorganic sulfate pool of the mouse applied to in vivo studies of sulfate incorporation into costal cartilage.


Studies on mammary respiratory metabolism and mammary development after thyroidectomy in the rabbit.


Regional differences in the incorporation rates of 3H-acetate and 35S-sulfate into chondroitin sulfate of mouse costal cartilage in vitro.


Regional distribution of red cells, plasma and blood volume in the intestinal wall of the cat.


Reflex relaxation of the stomach elicited from heart receptors.


Ventilatory responses to hypoxia and hypercapnia with external airway resistance.


Biochemistry of catecholamine storage: a comparison between sympathetic nerve trunk and adrenomedullary vesicles.


Renal vascular resistance in spontaneously hypertensive rats.


Secretory processes in follicular cells of the bat thyroid. I. Ultrastructural changes during the pre-, early and mid-hibernation periods with some comments on the origin of autophagic vacuoles.


Cytoarchitecture of the substantia nigra in the rat.


Morphological and physiological considerations in a new concept of calcium transport in bone.


Corticosteroid and bone.

Corticosteroid and bone.


Survival of patients with cystic fibrosis.


Evaluation of survivors of Respiratory Distress Syndrome at 4 years of age.


Age of menarche in girls in a West-South-Central community.


Epidemiologic investigation of hand, foot, and mouth disease. Infection caused by coxsackievirus A 16 in Baltimore, June through September 1968.


Congenital pulmonary lymphangiectasis.

Congenital pulmonary lymphangiectasis.


Dyschondrosteose. Mesomelic dwarfism of Lwei and Weill.


Radiological case of the month: acute hemorrhagic pancreatitis with recovery and formation of pancreatic pseudocyst.


Desquamative interstitial pneumonia in a 7-week-old infant.


Desquamative interstitial pneumonia in children. Report of two cases.


Quadriceps contracture in children.

Quadriceps contracture in children.


Congenital symmetric limb deformities.

Congenital symmetric limb deformities.


Probable homozygous beta thalassemia in a Negro child.


Acrocephalosyndactylia associated with a chromosomal translocation. 46,XX, t (2p-; Cq+).


Ventricular fibrillation caused by arsenic poisoning.


Gonadal dysgenesis in a newborn with XO karyotype.


Carbenicillin--a major new antibiotic.

Carbenicillin--a major new antibiotic.


Short-arm deletion, chromosome 4, (4p-), a syndrome?


The inheritance of translocations in man: data from families ascertained through a balanced heterozygote.


Atypical segregation of human red cell acid phosphatase phenotypes: evidence for a rare 'silent' allele Po.


Studies on the quantitative variation of human red cell peptidase A activity.


The relative activities attributable to the three phosphoglucomutase loci (PGM1, PGM2, PGM3) in human tissues.


Molecular size estimates of the human phosphoglucomutase isozymes by gel filtration chromatography.


The linkage relationships of adenosine deaminase.


Table of variance of ABO gene frequency estimates.


Fundamental theorem of natural selection in two loci.


Analysis of population structure. I. One-dimensional stepping-stone models of finite length.


Factors influencing the activity of succinate dehydrogenase in membrane preparations from Micrococcus lysodeikticus. 1. Some properties of succinate dehydrogenase [succinate-(acceptor) oxidoreductase, EC 1.3.99.1] in membrane preparations from Micrococcus lysodeikticus (N.C.T.C. 2665) were investigated. 2. In the spectrophotometric assay system adopted the reaction velocity was shown to be proportional to the amount of membrane added. Dichlorophenol-indophenol, reduced photochemically in the presence of phenazine methosulphate, or enzymically by the membrane-bound enzyme, was shown to undergo reoxidation in the dark. 3. The membrane-bound enzyme was found to be inactivated at temperatures above 10 degrees C. 4. The specific activity of membrane-bound succinate dehydrogenase was found to increase between two- and three-fold in diluted membrane preparations equilibrated at 0 degrees C for 6h. Membranes treated with sodium deoxycholate showed no enzyme activation on dilution but displayed maximal activity, all activity being sedimentable at 103000g. The increase in specific activity observed on dilution could be partially inhibited by fixation with glutaraldehyde, or by the presence of bovine serum albumin. 5. The addition of Mg(2+) or Ca(2+) ions to membrane suspensions caused an overall depression of enzyme activity. 6. The results suggest the presence of an ;inhibitor' that affects the expression of membrane bound succinate dehydrogenase activity.


Molecular hybridization between rat liver deoxyribonucleic acid and complementary ribonucleic acid. RNA (cRNA) was synthesized in vitro on a template of rat liver DNA and its hybridization with rat liver DNA was studied by using the nitrocellulose-filter method. Sonication of the DNA diminished its apparent capacity to hybridize with RNA by about 50%. This is not due to cross-linkage of DNA molecules, because it could be shown that less than 2% of the sonicated DNA was cross-linked. The effect is due instead to the small size of the sonicated DNA molecules. Below a single-stranded molecular weight of 5x10(5) the DNA showed a progressive loss of capacity to hybridize with decrease in molecular weight. Evidence is presented suggesting that the apparently diminished capacity of the DNA to hybridize is due to loss of hybridized DNA from the membrane filters. When cRNA at concentrations of up to 25mug/ml is annealed with sonicated total DNA, an apparent hybridization saturation value is found at which about 2.5% of the DNA is hybridized with RNA. Increasing the cRNA concentration tenfold brought about the hybridization of a second component of the DNA approximately equal in amount to the first. The renaturation of rat liver DNA was studied by measuring the fall in the extinction at 260nm and two different components of renaturation were observed within the reiterated fraction of DNA. By hybridizing cRNA with different fractions of rat DNA the two components of the hybridization curve are shown to correspond to the two components of the renaturation curve. The conclusion is drawn that at a cRNA concentration of 250mug/ml most of the reiterated fraction of rat liver DNA is hybridized after annealing for 16h under standard conditions (0.30m-sodium chloride-30mm-sodium citrate at 65 degrees C). Even with such a high cRNA concentration little or no hybridization of the slowly renaturing DNA fraction occurs. It is suggested that the most highly reiterated DNA component is poorly transcribed in vitro.


The chemistry of vitamin B 12. The co-ordination of biologically important molecules. The following equilibrium constants (given as logK in units of m(-1)) were determined for the substitution of co-ordinated H(2)O in aquocobalamin by glycine (bound through N) 5.8, cysteine (bound through S) 6.0 or 8.3, depending on the value chosen for the pK of the thiol group, and phenolate 2.9. The spectrum of the phenolate cobalamin shows an additional intense absorption band at 468nm with a molar extinction coefficient of 1.1x10(4), which is assigned to a charge transfer from the phenolate to the cobalt ion. Equilibrium constants have also been determined for the equilibria between adenylcobamide cyanide and CN(-), HO(-) and H(+), which show that the adenine is more easily displaced by CN(-) and HO(-) than is 5,6-dimethylbenziminazole in vitamin B(12), but can be protonated by acid while still remaining co-ordinated to the cobalt. It is shown that in the binding of corrinoids to proteins and polypeptides the formation of hydrogen bonds is far more important than co-ordination by the metal.


Effects of progesterone on protein metabolism in chicken oviduct tissue pretreated with oestrogen. 1. The effect of simultaneous injections of oestrogen benzoate and progesterone (0.5mg/day each) on immature chicken oviduct tissue pretreated with oestrogen benzoate (0.5mg/day) was studied. 2. After 3 days of treatment with both hormones, the weight of the tissue doubles as compared with tissue treated only with oestradiol benzoate. 3. The progesterone-induced weight increase had no effect on total DNA content of the tissue, but greatly increased the protein/DNA and RNA/DNA ratios. 4. Amino acid incorporation in vivo after progesterone treatment was elevated as measured by using 2h pulses of amino acids; this effect could be accounted for by observed alterations in the concentrations of free amino acids in the tissue. 5. With longer pulses of amino acid the specific radioactivity of total protein remained high in tissue treated with progesterone, at times when specific radioactivity of protein in oestrogen-treated animals was diminishing. 6. From a knowledge of the specific radioactivity of labelled amino acids in the free amino acid pool and in newly synthesized protein, the rate of protein synthesis was estimated in differently treated tissues. 7. The results suggest that progesterone treatment does not cause an increase in protein synthesis. It is concluded that the observed accumulation of oviduct protein is achieved via an effect of progesterone on protein catabolism.


The composition of triglycerides from liver, egg yolk and adipose tissue of the laying hen. The composition of the triglycerides of liver, egg yolk and adipose tissue of laying hens fed on a standard diet were investigated by using argentation thin-layer chromatography to separate the triglycerides according to their degree of unsaturation. About 40% of liver triglycerides consisted of one saturated and two monoenoic fatty acids. Triglycerides containing linoleate were more abundant in adipose tissue than in either yolk or liver. Hydrolysis by pancreatic lipase of the tissue triglycerides and fractions obtained from these triglycerides showed that the triglycerides of adipose tissue had a less ordered arrangement of fatty acids at the 2-position than did either yolk or liver triglycerides. The labelling patterns of triglycerides formed in liver slices incubated in the presence of [1-(3)(14)C]glycerol indicated that triglycerides containing four or more double bonds are formed to a greater extent than are other triglyceride fractions. This is evidence for the concept that the type of triglyceride formed depends on the availability of fatty acids to the liver cells.


Isolation and properties of conjugated bilirubin from bile. 1. A simple, rapid solvent partition method is described for isolation of conjugated bilirubin, free of unconjugated bilirubin, bile salts, phospholipids and cholesterol, from rat bile. Yields are 40-58%. The product is a phosphate-buffered solution containing approx. 0.4mg of bilirubin/ml, principally as mono- and di-glucuronide conjugates. The method may be modified for isolation of conjugates from human bile with 15-22% yield, and for preparation of unconjugated bilirubin from rat or human bile with yields of 55-62%. 2. The conjugated pigment has red-brown fluorescence and an absorption maximum at 450nm with in(mM) 59.8cm(-1). Diazotization by the Malloy-Evelyn method gives a direct Van den Bergh reaction (in water) 12% greater than the total reaction (in methanol), with in(total) 28.4x10(3)lmol(-1)cm(-1) at 550nm. After desalting by elution from Sephadex LH-20 in 50% (v/v) ethanol, the product gave water-soluble mustard-yellow crystalline needles. Such desalted conjugates were precipitated by Pb(2+) but not by Ba(2+), Ca(2+) or Zn(2+). 3. At pH7.0 and 37 degrees C the conjugated bilirubin was oxidized at a rate of 1%/h without hydrolysis, whereas 84% was hydrolysed by beta-glucuronidase or aqueous alkali. 4. Mono- and di-glucuronides were separated by elution from Sephadex LH-20 in 95% (v/v) ethanol or by extraction with chloroform at pH3.2-3.4. The monoconjugated bilirubin did not become labelled during incubation with unconjugated [(14)C]bilirubin, and chromatographed as a single spot without dissociating into unconjugated bilirubin and diglucuronide as would be expected of a complex. 5. After intravenous injection of mono- or di-conjugated [(14)C]bilirubin into normal or Gunn rats, 79-91% was excreted in bile and 2-7% in urine over 2h. In these experiments injected diglucuronide was not hydrolysed whereas 30-41% of injected monoglucuronide was converted into diglucuronide by the normal but not by the Gunn rats. The evidence favours the existence of a true bilirubin mono-glucuronide that is not a complex.


Tricarboxylic acid-cycle metabolism in brain. Effect of fluoroacetate and fluorocitrate on the labelling of glutamate, aspartate, glutamine and gamma-aminobutyrate. 1. The effect of fluoroacetate and fluorocitrate on the compartmentation of the glutamate-glutamine system was studied in brain slices with l-[U-(14)C]glutamate, l-[U-(14)C]aspartate, [1-(14)C]acetate and gamma-amino[1-(14)C]butyrate as precursors and in homogenates of brain tissue with [1-(14)C]acetate. The effect of fluoroacetate was also studied in vivo in mouse brain with [1-(14)C]acetate as precursor. 2. Fluoroacetate and fluorocitrate inhibit the labelling of glutamine from all precursors but affect the labelling of glutamate to a much lesser extent. This effect is not due to inhibition of glutamine synthetase. It is interpreted as being due to selective inhibition of the metabolism of a small pool of glutamate that preferentially labels glutamine.


Comparison of immunoadsorbents prepared by coupling sperm-whale myoglobin to a variety of insoluble polymers. Immunoadsorbents that were potentially suitable for the fractionation of antibodies to myoglobin were prepared by coupling myoglobin to aminoethylcellulose, bromoacetylcellulose, CM-cellulose, an ethylene-maleic anhydride co-polymer and Sepharose 4B. A comparison of the properties of the preparations indicated that Sepharose-myoglobin was superior, as an immunoadsorbent, to the other conjugates. Elution of adsorbed antibodies with m-propionic acid, pH2.5, gave yields of precipitable antibody higher than those achieved with other dissociating solvents. Propionic acid eluted 60-100% of the antibody adsorbed by Sepharose-myoglobin, and 60-90% of the eluted protein was precipitable antibody.


Decreased ribonucleic acid synthesis in isolated rat liver nuclei during starvation. 1. Isolated nuclei from starved rats showed a lowered incorporation of [(14)C]UMP into RNA. 2. The Mg(2+)-dependent incorporation was decreased by 30% after 1 day of starvation, but incorporation in the presence of Mn(2+) and ammonium sulphate decreased only after longer periods of starvation. 3. RNA synthesis by nuclei in the presence of excess of added RNA polymerase was unchanged after 1 day of starvation and was inhibited by 20% after 4 days. 4. The capacity of nuclei to bind actinomycin D was unchanged after 1 day and was decreased by 20% after 4 days of starvation.


The development of gluconeogenesis in rat liver. Effects of glucagon and ether. 1. Administration of glucagon to foetal rats produced a 10-15-fold increase in hepatic phosphoenolpyruvate carboxykinase activity together with a similar increase in the overall pathway of pyruvate conversion into glycogen in liver slices. 2. Glucagon was without effect on gluconeogenesis in vivo, which remained at approx. 0.1% of the incorporation as measured in newborn animals. 3. The apparent discrepancy between these results was due to the ether anaesthesia that was required for experimentation in vivo. Under conditions when minimal ether was used, the rates of labelling of glycogen from [3-(14)C]pyruvate in vivo were increased 10-20-fold and there was an additional stimulus by glucagon. 4. Ether anaesthesia produced a more reduced redox state of the foetal liver cytosol and lowered the ATP/ADP concentration ratio. 5. It is proposed that these effects are significant in the limitation of gluconeogenesis in the foetal rat liver, so that only with high phosphoenolpyruvate carboxykinase activity, high ATP concentration and a relatively oxidized cytosol redox state will a functional gluconeogenic pathway be present.


Identification of the acrylate porphyrin S-411 from meconium. A new porphyrin isolated from calf and foal meconium has been characterized as a tris-(2-carboxyethyl)-mono-(2-carboxyvinyl)-tetramethyl porphin, that is as an analogue of coproporphyrin in which one propionate group has been replaced by an acrylate group. The porphyrin is mainly a type III isomer and its possible significance in haem biosynthesis is discussed.


Carbon dioxide fixation in green sulphur bacteria. 1. About one-third of the CO(2) fixed during photosynthesis by washed suspensions of Chlorobium thiosulfatophilum strain 8346 gave rise to alpha-oxoglutarate and branched-chain oxo acids, mainly beta-methyl-alpha-oxovalerate. Another one-third to one-half gave rise to a polyglucose. 2. The fixation of CO(2) was inhibited by fluoroacetate, increasing concentrations up to 1mm stimulating the accumulation of alpha-oxoglutarate and causing a decrease in the formation of the branched-chain oxo acids and polyglucose. 3. Acetate was converted into the same products as was CO(2). 4. Fluoroacetate (1mm) had a negligible effect on the formation of polyglucose from acetate and caused a slight inhibition of the formation of the branched-chain oxo acids and increased accumulation of alpha-oxoglutarate. 5. Iodoacetate (1mm) strongly inhibited polyglucose formation from acetate and caused accumulation of pyruvate. The formation of the branched-chain oxo acids from acetate was only slightly affected by this inhibitor. 6. Pyruvate can be metabolized by this organism in the presence of a suitable electron donor whether CO(2) is present or not. In the absence of CO(2) pyruvate is converted into polyglucose. 7. The accumulation of oxo acids during CO(2) fixation is completely inhibited by NH(4) (+) ions. The formation of the branched-chain oxo acids is considerably decreased by the presence of isoleucine, leucine or valine, or a mixture of these. 8. CO(2) fixation in two other strains of Chlorobium appears to exhibit a similar pattern to that in C. thiosulfatophilum strain 8346. 9. It is concluded that in washed suspensions, CO(2) is fixed mainly by a mechanism involving the reductive carboxylic acid cycle. Acetate, the product of the cycle, is converted into polyglucose via pyruvate synthase and a reversal of glycolysis or into branched-chain oxo acids by an unknown mechanism.


Tamm-Horsfall urinary glycoprotein. The chemical composition. 1. A revised amino acid and carbohydrate composition of human Tamm-Horsfall glycoprotein is presented. 2. No significant differences were obtained in the amino acid composition of Tamm-Horsfall glycoprotein isolated from patients with cystic fibrosis. 3. The glycoprotein was shown to possess a high half-cystine content of 1 per 11-12 amino acid residues, which has been confirmed by performic acid oxidation and S-alkylation with iodoacetate and iodoacetamide. No thiol groups were detected in the glycoprotein. 4. Treatment of the glycoprotein with 0.5m-sodium hydroxide at 4 degrees C for 2 days did not release heterosaccharide material, which suggests that the predominant carbohydrate-protein linkages present are not of the O-glycosidic type. 5. No N-terminal amino acid was detected in the glycoprotein.


Effects of sex hormones on enzymic esterification of 2-(N-hydroxy-acetamido) fluorene by rat liver cytosol. 1. Enzymic esterifications of 2-(N-hydroxyacetamido)fluorene and several other hydroxamic acids by liver cytosol were studied. Determination of 2-acetamido-3-methylthiofluorene was used for the assay. 2. With rat liver cytosol, requirement for ATP, Mg(2+) and SO(4) (2-) suggested formation of phosphate and sulphate esters of 2-(N-hydroxyacetamido)fluorene. 3. Rats showed sex and age differences in their activity. Liver from adult male rat was at least twice as active as liver from adult female rat. No such sex differences were found in mice, hamsters and guinea pigs. 4. Administration of testosterone (300mug/day) subcutaneously for 8 days increased the activity in the female rat by 100%, whereas diethylstilboestrol (100mug/day) had no effect. In the male rat diethylstilboestrol treatment decreased the activity by 60%, whereas testosterone pretreatment was without any effect. 5. Among various endocrine ablations such as adrenalectomy, castration, adrenalectomy-castration and hypophysectomy in the adult male rat, hypophysectomy was found to be the most effective in decreasing the activity of the liver to about 50% of control values. 6. Like 2-(N-hydroxyacetamido)fluorene, various other N-hydroxy derivatives of 2-acetamido-7-fluorofluorene, 2-acetamidophenanthrene, 4-acetamidobiphenyl and 4-acetamidostilbene were also shown to be esterified to different extents by rat liver cytosol.


Utilization of glycerol by rat adipose tissue in vitro.


Tamm-Horsfall urinary glycoprotein. The subunit structure. 1. Subunit molecular weights of 76000-82000 were obtained for native and alkylated Tamm-Horsfall glycoprotein by gel filtration on Sephadex G-200 in the presence of sodium dodecyl sulphate. 2. A further estimate of the subunit molecular weight of 79000+/-4000 was obtained by disc gel electrophoresis in sodium dodecyl sulphate. 3. A minimum value of the chemical molecular weight of 79000+/-6000 was obtained from the number of N-terminal amino acids released by cyanogen bromide cleavage of the glycoprotein. 4. Similar values were obtained for the subunit molecular weight of Tamm-Horsfall glycoprotein from patients with cystic fibrosis. 5. On ultracentrifugation both in 1.0% sodium dodecyl sulphate and in 70% formic acid, Tamm-Horsfall glycoprotein sedimented as a single component, slightly faster than serum albumin. 6. On reduction of the disulphide bonds the same subunit molecular weight was obtained, which suggested that these bonds are intrachain.


Deoxyribonucleoproteins and the tissue-specific restriction of the deoxyribonucleic acid in chromatin.


The structure of 'phosphatidylglucose'.

The structure of 'phosphatidylglucose'.


Acute gastroenteritis in African and European adults in Rhodesia.


Anomalies in the pathways of medial and lateral popliteal and radial nerves.


Cholera.

Cholera.


Oesophageal stricture in scleroderma.

Oesophageal stricture in scleroderma.


Estimation of 5-hydroxyindolylacetic acid in sheep urine.


A rapid enzymic method for glycogen estimation in very small tissue samples.


A sensitive and specific fluorimetric method for the determination of noradrenalin and adrenalin in human plasma.


A simplified method for the estimation of urinary free cortisol and 20-dihydrocortisol by fluorometry.


Fractionation and properties of the protease inhibitors of effusions.


Natural evolution and pathological alterations of lymphoid cell proteins. Electrophoretic patterns of soluble and insoluble components.


Fluorimetric determination of serum 11-hydroxysteroids. Calculative correction of deviations in complex fluorescence spectra.


A sensitive micromethod for the determination of methemoglobin in blood.


Gas-chromatographic estimation of 3-methoxy-4-hydroxy phenylglycol after transformation to vanillin methoxime trimethylsilyl ether.


Spectrophotometric study of bilirubin interference in the Huang reaction for cholesterol.


Disaccharidase levels in jejunal biopsy specimens from American and south Indian control subjects and patients with tropical sprue.


Failure to detect trans-3-methyl-2-hexenoic acid in the sweat of schizophrenic patients.


pH dependency of the cyanide-nitroprusside colour reaction of cystine.


Increased protocollagen proline hydroxylase activity in synovial tissue in rheumatoid arthritis.


Nonketotic hyperglycinaemia. Clinical findings and amino acid analyses on the plasma of a new case.


The determination of bromsulphthalein in serum.


A simplified one-stage cation exchange technique for the determination of whole blood ammonium.


A simple method for serum arginase determination.


Lipoprotein lipase in clinical and experimental nephrosis.


Comparison of membrane filtration and nephelometry with analytical ultracentrifugation, for the quantitative analysis of low density lipoprotein fractions.


The influence of air and natural gas flow rates on interferences in atomic absorption spectroscopy.


Presence in Vicia faba of different substances with activity in vitro on Gd(-)Med red blood cell reduced glutathione.


Modification of the electrophoretic mobility of serum alkaline phosphatase by acetylation.


The effect of pH on the uptake of methylene blue by human red cells.


Fetal physiology. Foreword.

Fetal physiology. Foreword.


Improvement of a new SGOT and SGPT ultramicro method. Calibration procedure.


Intrauterine growth retardation.

Intrauterine growth retardation.


Fetal malnutrition.

Fetal malnutrition.


Thermal and metabolic features of life in utero.


Umbilical blood flow and fetal oxygen consumption.


Fetal glucose metabolism.

Fetal glucose metabolism.


Physiological aspects of amino acid transport across the placenta.


Fetal acid-base homeostasis.

Fetal acid-base homeostasis.


Fetal brain metabolism and function.

Fetal brain metabolism and function.


Psychosexual problems in obstetrics and gynecology. Foreword.


Motivations for coitus.

Motivations for coitus.


Contraception.

Contraception.


The unwanted pregnancy.

The unwanted pregnancy.


Problems of teen-agers presented to obstetricians and gynecologists.


Psychosexual disorders of young adulthood.


Psychosexual problems of the middle years.


Recognition of psychosocial factors.

Recognition of psychosocial factors.


The physician's role as counselor: when, where and how to make referrals.


Salt, vascular reactivity and hypertension.


Interstitial fluid pressure measured with a cotton wick.


Fibrinogen derivatives in renal disease.


The effect of the spirolactone SC 14266 on the sodium transport across isolated frogskin.


The effect of spironolactone on sodium transport in the absence of aldosterone.


The effect of a high intake of calcium and phosphate in normal subjects and patients with chronic renal failure.


Growth changes in developing nerves.

Growth changes in developing nerves.


The aetiology of optic neuritis.

The aetiology of optic neuritis.


Effects of standard intravenous insulin tolerance tests on growth hormone, 11-hydroxycorticosteroids, free fatty acids and plasma sugar in myxoedema.


Amino acid and peptide uptake in protein absorption.


Effect of speed of inspiration on gas distribution in lungs of normal subjects and patients with bronchitis.


Intestinal absorption and biopsy transport of peptides and amino acids in Hartnup disease.


The effects of decreased thoracic compliance on the ventilatory response to CO2.


The oxygen affinity of haemoglobin in chronic renal failure.


Extravascular albumin in human tissues.

Extravascular albumin in human tissues.


Relationships between potassium, chloride, intracellular and extracellularH in dogs.


Effort and the forced expiratory volume in one second (FEV).


The influence of pH, bicarbonate and hypertonicity on the absorption of ammonia from the rat intestine.


The absorption of glycine and glycine oligopeptides by the rat.


Vascular hyper-reactivity with sodium loading and with desoxycorticosterone induced hypertension in the rat.


Different effects of frusemide administered during hypertonic saline infusion in healthy subjects and hypertensive patients.


University curriculums AND FELLOWSHIPS IN RADIOLOGICAL HEALTH.


Studies on nucleolar liver DNA.

Studies on nucleolar liver DNA.


Interaction of haemoglobin with ions. Thermodynamic data on binding of adenosine triphosphate to methaemoglobin.


The structure of legumin, a storage protein of broad bean (Vicia faba) seed.


The essential thiol group of arginine kinase from Homarus vulgaris muscle studied by difference spectrophotometry and N-ethyl-[1-14C] maleimide labelling.


Heterogeneity of horse liver alcohol dehydrogenase. Purification and characterization of multiple molecular forms.


Phylogeny of the neurohypophysial hormones. The avian active peptides.


Diaminodurene-induced plastocyanin dependent oxygen uptake and its relation to photophosphorylation in isolated lettuce chloroplasts. A comparison of the systems using either water or ascorbate as the electron donors.


Structural comparison of ovalbumins from nine different species.


The effects of magnesium and manganese on the purine ratio of RNA transcribed in vitro by rat liver nuclei.


Hydrocarbon-utilizing microorganism. Relationship of fatty acid composition and biosynthesis to hydrocarbon substrate and to vitamin B 12 level in Corynebacterium simplex.


Chromatographic separation of membrane proteins on lipophilic ion exchange resins. The influence of various resin-linked aliphatic chains.


3-phosphoglycerate kinase from rabbit sceletal muscle and yeast.


pH-dependence of the ceruloplasmin catalyzed oxidation of dimethyl-p-phenylenediamine.


Thermal behaviour of variously acclimatized individuals of Polydesmus complanatus L. (Diplopoda) in a temperature gradient.


The effect of testosterone on the weight and fructose contents of the accessory glands in males of two inbred strains of mice and their F1 hybrids.


Morpho-functional study of hypothalamic neurosecretion. IV. The effect of osmotic stress on the preoptico-neurohypophysial system of Rana esculenta, Rana temporaria, and Xenopus laevis.


Morpho-functional study of hypothalamic neurosecretion. V. The effect of light and temperature in Rana temporaria.


Mortal wounds and pre-experientially differentiated social attitudes in white mice.


Morpho-functional study of hypothalamic neurosecretion. VI. The effect of locally administered X-irradiation in Rana temporaria and Rana esculenta.


Neurosecretion in adult Salamandra salamandra L.


Serum cholesterol concentrations in newborn babies and mothers.


Isosorbide di-nitrate in angina pectoris. A double-blind-study of 50 cases.


Use of aminophylline in the treatment of heart disease.


Electrode catheter in the diagnosis of Ebstein's disease.


Serum non-esterified fatty acids after acute myocardial infarction.


Pericarditis complicating congenital dextrocardia with situs inversus.


A case of essential hypercholesterolaemic xanthomatosis with myocardial infarction.


Intermittent bundle branch block and complete heart block in Marfan's syndrome.


A case of pseudo pulmonary stenosis.

A case of pseudo pulmonary stenosis.


Ulcerated lupus erythematosus.

Ulcerated lupus erythematosus.


India, January-February 1970.

India, January-February 1970.


Erythema nodosum and psittacosis pneumonia. A report of an unusual clinical association.


Studies on two types of glutaminase in normal and tumour bearing mice.


Abnormal haemoglobins in Indian armed forces personnel.


Follow up study of 120 cases of stone in the upper urinary tract.


Determination of the functional extracellular fluid volume (ECFV) by sodium-24 chloride.


Some aspects of collagen metabolism in leprosy.


Adrenergic neurone blockade with chloroquine and amodiaquine.


Prevalence of trachoma and other common eye diseases.


Mathematical models in the assessment of infective force in filariasi.


An analytical study of the indices of thermal stress with respect to human body temperature.


Tuberculosis of gastroin-intestinal tract: with special reference to rectal tuberculosis.


Coxsackie B3 virus myocarditis in India.


Globoid leukodystrophy (Krabbe's disease).


The organisation of medical work in hospitals.


I.M.A. Maternal Mortality Committee. Report for 1969.


Controlled intralesional deposition of a sclerotic agent in verruca therapy.


Diagnostic abdominal paracentesis in the acute abdomen.


Skin changes in protein calorie deficiency malnutrition.


Threatened gangrene in pregnancy caused by root alkaloids.


Psoriasiform lupus vulgaris: a case report.


The radiological diagnosis of pericardial effusion using intravenous C2.


Pseudoxanthoma elasticum with a sarcoidal granulomatous reaction: a case report.


Skin diseases among soldiers evacuated from Southeast Asia.


Frameshift mutation in the lysozyme gene of bacteriophage T4: demonstration of the insertion of five bases, and a summary of in vivo codons and lysozyme activities.


Structure of crystalline alpha-chymotrypsin. IV. The structure of indoleacryloyl-alpha-chyotrypsin and its relevance to the hydrolytic mechanism of the enzyme.


The distribution of nucleic acids in the giant axon of the squid (Loligo pealii).


Lichen amyloidosus: a case report.

Lichen amyloidosus: a case report.


Phagedaenic tuberculous ulcers of the upper arm: report of three cases.


Decrease of cerebral serotonin and 5-hydroxyindolylacetic acid caused by (-)-alpha-methyltryptophan.


Synthesis de novo of purines in slices of rat brain and liver.


Isolation and identification of alpha-aspartyl-serine, alanylphenylalanine and isoleucylleucine from calf brain stem.


Distribution of amino acids and of exo- and endopeptidases along vertebrate and invertebrate nerves.


Diaminodiphenylsulfone and steroids in the treatment of pyoderma gangrenosum.


Gummatous sporotrichosis.

Gummatous sporotrichosis.


Creatine kinase from brain: kinetic aspects.


Composition of cerebral lipids in murine sudanophilic leucodystrophy: the Jimpy mutant.


Fatty acids of lecithin in subcellular fractions during maturation of brain.


Study of RNA in subcellular fractions from rat brain: simultaneous incorporation of [14C]uridine and [3H]methyl-L-methionine.


The free amino acids in human cerebrospinal fluid.


Fucose incorporation into glycoproteins of mouse brain.


Mercaptopropionic acid: a convulsant that inhibits glutamate decarboxylase.


The enzymic acetylation of choline analogues.


On the origin of the acetyl moiety of acetylcholine in brain studied with a differential labelling technique using 3H-14C-mixed labelled glucose and acetate.


Formation and turnover of myelin ganglioside.


Neurochemical and structural studies on the mechanism of action of hemicholinium-3 in central cholinergic synapses.


A new procedure for the measurement of acid-soluble glutamine of tissue with particular reference to the brain.


Reversible changes in glutamine levels in the cat cerebral cortex evoked by afferent electrical stimulation and by administration of pentamethylenetetrazole (pentylenetetrazol).


Effects of shock and anoxia on nucleotides and creatine phosphate in the isolated brain of the dog.


Depletion of biotin from brain and liver in biotin deficiency.


Effects of 6-hydroxydopamine on catecholamine containing neurones in the rat brain.


Piperidine in the cerebellum of the dog.


Hexose and pentose phosphates in brain during convulsions.


The disc electrophoretic separation of proteins from various parts of the guinea pig brain.


Synthesis of RNA in developing rat brain in vitro.


RNA in single identified neurons of aplysia.


Cerebral lipids in a case of systemic Gm2-gangliosidosis of a late infantile type.


Brain lipid modifications induced by essential fatty acid deficiency in growing male and female rats.


Sphingolipids of developing human central nervous tissue: changes in composition of sphingosine bases.


Further experiments on the uptake of acetylcholine and atropine and the release of acetylcholine from mouse brain cortex slices after treatment with phospholipases.


Biosynthesis of lactosylceramide by rat brain preparations and comparison with the formation of ganglioside Gm1 and psychosine during development.


Composition of acyl groups in the neutral glycerides from mouse brain.


Failure to detect 5-hydroxytryptamine-O-sulphate in normal rat brain and after monoamine oxidase inhibition.


Incorporation of (14C)glutamate into glutathione in rat brain.


Changes in brain polyribosomes following an electro-convulsive seizure.


Farmer's lung, its geographic distribution.


Current concepts of tuberculosis control and treatment.


Pre-employment history for disability rating prediction.


Changing technology in the coal industry--industrial hygiene needs.


Coal workers' pneumoconiosis: medical research needs.


Instant benzol death.

Instant benzol death.


Health and safety reminder.

Health and safety reminder.


Chemical structure of polyurethane foam.


Wasp and bee stings in children.

Wasp and bee stings in children.


Congenital rubella in Singapore.

Congenital rubella in Singapore.


Osmolality of the urine of newborn infants.


Varicella myelopathy.

Varicella myelopathy.


The effect of orally administered zinc sulfate (ZNSO4) on healing incised wounds.


Physiologic shunting in the lung in critically ill or injured patients.


Pancreatic digestive enzymes: influence of a diet that augments pancreatitis.


The isolation and identification of the numbing principle in Chrysanthemum anethifolium.


Experience with large vessel applique (Thomas) shunts for haemodialysis.


Management of patients unconscious from drug overdosage.


Possible genetic bsis of childhood neoplasia.


Fat embolism synrome.

Fat embolism synrome.


Torsion of the hydatid of Morgagni in two brothers.


Heptolenticular degeneration (Wilson's disease) in an Australian Aboriginal.


Service to people in the 1970's.

Service to people in the 1970's.


Psychiatric indications for the termination of pregnancy.


Intestinal absorption of oligopeptides.

Intestinal absorption of oligopeptides.


The treatmet of asthma.

The treatmet of asthma.


Snake-bite.

Snake-bite.


Clinical pharmacology: a bridge to better treatment.


Anxiety and pain.

Anxiety and pain.


Intertrochanteric fractures of the femur.


Plastic surgery evaluation report. II.

Plastic surgery evaluation report. II.


Biliary tract disorders; personal observations in 90 consecutive patients.


Antrostomy. II.

Antrostomy. II.


Acute alcoholism and the withdrawal state.


Fractures of the 2nd cervical vertebra; neurosurgical management.


Gallstone ileus.

Gallstone ileus.


Paroxysmal tachycardia.

Paroxysmal tachycardia.


An evaluation of the activity of commercial cellulolytic and pectolytic enzymes.


The characterisation of the form of lactose in spray dried lactose.


The effects of moisture on the flow properties and compression of phenacetin, paracetamol and dextrose monohydrate.


The quantitative determination of atropine in presence of its hydrolysis and dehydration products.


Spinal muscular atrophy of the scapulo-peroneal-type.


The interaction of triethyltin with a component of guinea-pig liver supernatant. Evidence for histidine in the binding sites. A protein fraction was isolated from guinea-pig liver that binds triethyltin with an affinity of approx. 2x10(6)m(-1) at pH8.0. It was shown that the protein responsible for binding 70% of the triethyltin found in guinea-pig liver after injection of radioactively labelled triethyltin is at most a few per cent of the total liver protein. Evidence is presented from the kinetics of loss of binding and loss of certain amino acids on photo-oxidation with either Methylene Blue or Rose Bengal that each binding site consists of two histidine residues.


The preparation of alpha-oxo acid derivatives suitable for specific-radioactivity determination. 1. Conditions favouring the condensation of o-phenylenediamine with alpha-oxo acids to form substituted quinoxalines have been investigated. The best yields (85-100%) are obtained by incubation of the reagents in 2m-hydrochloric acid. Substitution of acetic acid for hydrochloric acid, or of halogen-substituted 1,2-diaminobenzenes for phenylenediamine, increases the initial rate of reaction, but not the yield of quinoxalines. 2. The quinoxaline derivatives are insoluble in water and can be readily purified. They are very suitable for determination of the specific radioactivities of radioactively labelled alpha-oxo acids because they are colourless, soluble in dioxan-based scintillators, and their strong absorption of u.v. light facilitates their chemical determination. As little as 1 nmol can be accurately determined by means of the blue fluorescence produced by absorption of light at 340-360nm. 3. Optimum conditions for preparation, purification and separation by paper and thin-layer chromatography are described.


Preparation and properties of creatine kinase from the breast muscle of normal and dystrophic chicken (Gallus domesticus). 1. The purification of creatine kinase from normal and genetically dystrophic chicken breast muscle is described. Enzyme recovery was significantly lower from dystrophic muscle. 2. Both enzymes had the same number of reactive and total thiol groups and had similar specific activities and similar amino acid compositions. 3. No significant differences were observed in sedimentation, electrophoretic or kinetic properties. 4. Peptide ;maps' showed no significant differences, and electrophoresis of partial acid hydrolysates of the labelled enzymes suggested that corresponding amino acid sequences around all the thiol groups were very similar. 5. The enzymes showed identical temperature stabilities. 6. No significant differences between the enzymes from normal and dystrophic muscle were observed.


The preparation and properties of yeast beta-fructofuranosidase chemically attached to polystyrene. 1. A process is described for the chemical attachment of an enzyme to the surface of a polystyrene matrix. 2. By this process yeast beta-fructofuranosidase was chemically attached to the surface of both polystyrene beads and polystyrene tubes. 3. The kinetics of sucrose hydrolysis by beta-fructofuranosidase and polystyrene-supported beta-fructofuranosidase were compared. 4. The pH-activity curve of the polystyrene-supported enzyme shows a marked difference from that of the free enzyme in solution. 5. The inhibitor dissociation constant with respect to tris is increased, whereas the inhibitor dissociation constant with respect to aniline is decreased when the enzyme is attached to polystyrene. 6. Differences between the properties of the bound and free enzyme are discussed in terms of a micro-environmental effect arising from the hydrophobic nature of the polystyrene support.


The subcellular localization of di- and tri-peptide hydrolase activity in guinea-pig small intestine. 1. Two different subcellular fractionation techniques were applied to guinea-pig intestinal mucosa and the composition of the brush borders prepared by the two methods were compared. 2. By using a kinetic assay system the subcellular distribution of activity against ten dipeptides and five tripeptides was studied. 3. Only small amounts (5-10%) of activity against dipeptides were found in the brush-border region, the enzymes being concentrated in the cytosol. 4. Significant amounts (10-60%) of activity against tripeptides were found in the brush border with the remainder largely present in the soluble fraction. 5. The relevance of these studies to the localization in vivo and the possible role of peptidases in protein digestion is discussed.


The effect of feeding with a tryptophan-free amino acid mixture on rat liver magnesium ion-activated deoxyribonucleic acid-dependent ribonucleic acid polymerase. 1. The Widnell & Tata (1966) assay method for Mg(2+)-activated DNA-dependent RNA polymerase was used for initial-velocity determinations of rat liver nuclear RNA polymerase. One unit (U) of RNA polymerase was defined as that amount of enzyme required for 1 mmol of [(3)H]GMP incorporation/min at 37 degrees C. 2. Colony fed rats were found to have a mean RNA polymerase activity of 65.9muU/mg of DNA and 18h-starved rats had a mean activity of 53.2muU/mg of DNA. Longer periods of starvation did not significantly decrease RNA polymerase activity further. 3. Rats that had been starved for 18h were used for all feeding experiments. Complete and tryptophan-deficient amino acid mixtures were given by stomach tube and the animals were killed 15-120min later. The response of RNA polymerase to the feeding with the complete amino acid mixture was rapid and almost linear over the first hour of feeding, resulting in a doubling of activity. The activity was still elevated above the starvation value at 120min after feeding. The tryptophan-deficient amino acid mixture produced a much less vigorous response about 45min after the feeding, and the activity had returned to the starvation value by 120min after the feeding. 4. The response of RNA polymerase to the feeding with the complete amino acid mixture was shown to occur within a period of less than 5min to about 10min after the feeding. 5. Pretreatment of the animals with puromycin or cycloheximide was found to abolish the 15min RNA polymerase response to the feeding with the complete amino acid mixture, but the activity of the controls was unaffected. 6. The characteristics of the RNA polymerase from 18h-starved animals and animals fed with the complete or incomplete amino acid mixtures for 1h were examined. The effects of Mg(2+) ions, pH, actinomycin D and nucleoside triphosphate omissions were determined. The [Mg(2+)]- and pH-activity profiles of the RNA polymerase from the animal fed with the complete mixture appeared to differ from those of the enzyme from the other groups, but this difference is probably not significant. 7. [5-(3)H]Orotic acid incorporation by rat liver nuclei in vivo was shown to be affected by the amino acid mixtures in a similar manner to the RNA polymerase. 8. The tryptophan concentrations of plasma and liver were determined up to 120 min after feeding with the amino acid mixtures. Feeding with the complete mixture produced a rapid increase in free tryptophan concentrations in both plasma and liver, but feeding with the incomplete mixture did not alter the plasma concentration. The liver tryptophan concentration increased at about 45min after feeding with the tryptophan-deficient diet. 9. There was a good correlation between the liver tryptophan concentration and RNA polymerase activity in all groups of animals. 10. It was concluded that the rat liver nucleus responded to an increase in amino acid supply by increased synthesis of RNA as a result of synthesis of RNA polymerase de novo. The correlation of tryptophan concentration and RNA polymerase activity appears to reflect the general amino acid concentration required to support hepatic protein synthesis and to produce new RNA polymerase. This new polymerase appears to differ from the basal RNA polymerase by its rapid synthesis and destruction, which may be a means of regulating RNA synthesis by the amino acid concentration in the liver.


Fatty acid utilization during development of the rat. 1. In the absence of added carnitine pronounced changes occur during development, in the degradation of [U-(14)C]palmitate to (14)C-labelled acid-soluble material (predominantly ketone bodies) and (14)CO(2) by liver homogenates. The formation of both products by liver homogenates of 5-day-old rats is considerably higher than in foetal livers. This high value is maintained during the suckling period and falls after weaning to adult values which are similar to those of the foetus. Addition of carnitine stimulates production of acid-soluble material throughout development. The effect of added carnitine on CO(2) production varies with age, stimulating in foetal and post-weaning age groups but inhibiting during the suckling period. 2. The degradation of [U-(14)C]palmitate to acid-soluble material and CO(2) by heart homogenates also varies during development. Formation of both products is highest 5 days after birth and thereafter decreases steadily to adult values. Addition of carnitine stimulates the production of both CO(2) and acid-soluble material at all ages. 3. The activity of carnitine palmitoyltransferase in liver increased after birth to reach maximum activity at 2-5 days of age. Thereafter the activity of the enzyme decreased until day 15 of life and then remained constant until day 30, decreasing after this time to adult values which were about one half of the foetal liver activities.


Tetrodotoxin-sensitive uptake of ions and water byslices of rat brain in vitro. 1. Under certain conditions the processes that bring about increased Na(+) influx and water uptake into rat brain slices have a tetrodotoxin-sensitive component. 2. Incubation of slices in the absence of glucose, or in the presence of ouabain (0.1mm), protoveratrine (10mum) or electrical stimuli, leads to increased uptakes of water and Na(+) that are partially suppressed by tetrodotoxin (3mum). 3. The increased water uptake and Na(+) influx due to the presence of 30mum-2,4-dinitrophenol or of 100mm-potassium chloride are unaffected by tetrodotoxin. 4. The additional presence of 27mm-potassium chloride diminishes, or abolishes, the inhibitory effects of tetrodotoxin on the increased uptakes of water and Na(+) found on incubation in the absence of glucose or in the presence of protoveratrine. 5. Ouabain increases Na(+) influx into caudate nuclei from rat brain and this process is suppressed by tetrodotoxin. Ouabain does not increase water uptake in the caudate nuclei. 6. Diminution of Na(+) influx in slices due to tetrodotoxin is not accompanied by an equal efflux of K(+). 7. It is concluded that glial-cell membranes are permeable to K(+) and Cl(-) and, as a consequence, when K(+) is released from neurons in association with action potentials it, together with water, moves into the glial cells. The major consequence of K(+) (and Cl(-)) permeability of the glial cell is therefore a buffering of the extracellular K(+) concentration. 8. l-Glutamate presents an anomalous feature that may indicate that its effects are not restricted to neurons. 9. Calculations are made of the increase of K(+) flux into the glia in the presence of ouabain or of protoveratrine or in the absence of glucose or on application of electrical impulses.


The enzymic conversion of linoleic acid hydroperoxide by flax-seed hydroperoxide isomerase. 1. The mode of action of flax-seed hydroperoxide isomerase was studied in vitro by using as substrates linoleic acid hydroperoxides formed by soya-bean lipoxygenase. 2. The enzyme converts only 13-hydroperoxyoctadeca-cis-9-trans-11-dienoic acid, whereas the 9-hydroperoxy isomer does not react. 3. The isomerization product was identified by chemical and spectroscopic methods as 13-hydroxy-12-oxo-octadec-cis-9-enoic acid. 4. 12,13-Epoxyoleic acid isomers do not act as intermediates in the isomerization reaction. 5. Suggestions for a functional relationship between hydroperoxide isomerase and lipoxygenase are discussed.


Physiology of the reproductive cycle in the cockroach Byrsotria fumigata (Guérin).


Experimental studies on the endocrinology and reproductive biology of the viviparous polychaete annelid, Nereis limnicola Johnson.


The separation of beta-glucanases produced by Cytophaga johnsonii and their role in the lysis of yeast cell walls. 1. When Cytophaga johnsonii was grown in the presence of suitable inducers the culture fluid was capable of lysing thiol-treated yeast cell walls in vitro. 2. Autoclaved or alkali-extracted cells, isolated cell walls and glucan preparations made from them were effective inducers, but living yeast cells or cells killed by minimal heat treatment were not. 3. Chromatographic fractionation of lytic culture fluids showed the presence of two types of endo-beta-(1-->3)-glucanase and several beta-(1-->6)-glucanases; the latter may be induced separately by growing the myxo-bacterium in the presence of lutean. 4. Extensive solubilization of yeast cell walls was obtained only with preparations of one of these glucanases, an endo-beta-(1-->3)-glucanase producing as end products mainly oligosaccharides having five or more residues. Lysis by the other endo-beta-(1-->3)-glucanase was incomplete. 5. The beta-(1-->6)-glucanases produced a uniform thinning of the cell walls, and mannan-peptide was found in the solution. 6. These results, and the actions of the enzyme preparations on a variety of wall-derived preparations made from baker's yeast, are discussed in the light of present conceptions of yeast cell-wall structure.


Interaction of food and photoperiod in the termination of larval diapause in Chaoborus americanus (Diptera: Culicidae).


Dixenic culture of Daphnia magna, Straus.


Sensitivity of echolocation in cave swiftlets.


The role of amoebocytes in the regeneration of shell in the land pulmonate, Euplecta indica (Pfieffer).


"Distance effect" in pigeon orientation: an evaluation.


Control of molting in mandibulate and chelicerate arthropods by ecdysones.


Gastrulation in the turbellarian Hydrolimax grisea (Platyhelminthes; Plagiostomidae): formation of the epidermal cavity, inversion and epiboly.


Larval development of Pagurus annulipes (Stimpson, 1862) and Pagurus pollicaris Say, 1817 reared in the laboratory.


Effects of subzero temperatures and trawling stress on serum osmolality in the winter flounder Pseudopleuronectes americanus.


Extradural haematoma of the posterior fossa. Report of three cases with review of the literature.


Incidence of bronchial adenoma in Ceylon, with case report in a child.


Scarring alopecia.

Scarring alopecia.


Aetiological factors of cirrhosis of the liver in Ceylon. (A study of 206 cases).


Leptospiral problems in pregnancy.

Leptospiral problems in pregnancy.


Towards the development of effective occupational health services in Ceylon.


Like-spaces versus bailiwick approaches to the design of health care facilities.


Quantitative methods for evaluating hospital designs. Concepts and plan of research.


Quantitative methods for evaluating hospital design. A stochastic model for interdepartment traffic.


Quantitative methods for evaluating hospital design. A methodology for total hospital design.


Spoke design for inpatient care.

Spoke design for inpatient care.


Spoke design for inpatient care. Designing for nursing efficiency: a multidisciplinary evaluation.


Effect of spoke design on occupancy: a simulation model.


Spoke design for inpatient care. Hospital Director's Postscript.


Spoke design for inpatient care. Evaluation of patient welfare.


Design strategy for flexible health sciences facilities.


Influence of altered hormonal states on mast cells of the rat prostatic complex.


Effects of urethane on embryonic development in the mouse. I. The period of organogenesis.


Effect of cadmium chloride on the ovary of the Indian desert gerbil, Meriones hurrianae Jerdon.


Hypothalamo-neurohypophysial complex of the teleost, Heteropneustes fossili(Bl.), with some experimental evidence on the regeneration of the neurosecretory tract.


Histological studies of the neuroendocrine system of Gryllodes sigillatus (Walk.) in relation to wing development.


Comparative evaluation of three methods for extraction of gonadotrophins from buffalo pituitaries.


Experimental atherosclerosis: sensitivity of rabbit aortic strips to norepiphrine.


Occurrence of histamine in the intestines of rats harbouring cysticercoids of Hymenolepis nana.


Sensitivity of male rats of different strains to vasopressi.


Major constituents of the conidial wall of Neurospora crassa.


Effect of ganglion blocking agents on spontaneous & adrenaline-induced ventricular ectopic tachycardia following coronary occlusion.


Microspectrophotometric studies on the pigments in vivo of singlelgal cells. II. Multiple forms of chlorophyll in Scenedesmus obliquus& Chlamydomonas sp.


Effect of myocardial stimulants on the acetylcholine content of the heart.


Effect of acute & chronic ethanol administration on blood glucose & glucose tolerance of white rats.


Effect of melengestrol acetate on the bleeding & clotting times of blood in Indian water buffalo.


Lipid metabolism in relation to reproduction & moulng in the crab, Paratelphusa hydrodromous (Herbst): phospholipids & reproduction.


Nature of the pigments of millipede, Arthrosphaera lutescens Butler.


Influence of carbon & nitrogen sources on the biosynthesis of litmocidin by a new strain of Streptomyces litmocidini.


Insecticide & larvicide activity in the extract of Piper peepuloides Royle (Piperaceae).


Changes in morphological characters during germination of gamma-irradiated castor seeds.


Metronidazole in trichomoniasis.

Metronidazole in trichomoniasis.


Genito-urinary richomoniasis.

Genito-urinary richomoniasis.


Lactic acid, an index of diabetes mellitus.


Myocardial injury after scorpion ting.

Myocardial injury after scorpion ting.


Intramedullary tuberuloma of the spinal cord. A case with a five year follow-up.


Serum caeruloplasmin in vitiligo.

Serum caeruloplasmin in vitiligo.


Anemia. Hemolytic anemia. 3.

Anemia. Hemolytic anemia. 3.


Contrast perception in imaging systems.

Contrast perception in imaging systems.


Effect of the beta spectrum on self-absorption of beta radiation from 14C.


The absolute measurement of beta-disintegrating nuclides emitting conversion electrons with appreciable proportions.


The determination of gadolinium in irradiated gadolinia-magnesia mixtures.


An auxiliary cyclotron beam monitor.

An auxiliary cyclotron beam monitor.


Electron induced luminescence in radiation standards of tritium and radiocarbon in acryl plastics.


Cyclotron production of carrier-free gallium-67.


Intercellular attachments of cockroach nymph epidermal cells.


Ultrastructure of striated inclusions in Neurospora.


Surface topology of the stomach in man and the laboratory ferret.


Stereological analysis of the ultrastructure of liver parenchymal cells during pregnancy and lactation.


Ultrastructure of Mycoplasma gallisepticum isolate 1056.


Application of an electrochemical preconcentration technique to neutron activation analysis of trace metal ions.


Nucleocytoplasmic interaction at the nuclear envelope in post meiotic microspores of Pinus banksiana.


Transmission electron microscopy and stereo ultrastructure of the T system in frog skeletal muscle.


Development of spermatozoa in the wooly opossum with special reference to the shaping of the sperm head.


Ultrastructure of spermatozoa of the wooly opossum Caluromys philander.


Supraglottal air pressure in the production of English stops.


Concept learning and the short-term memory--a contribution to theory.


General language deficits in children with articulation problems.


Structural apperception in the absence of syntactic constraints.


The articulation test as a linguistic technique.


Paralinguistic and kinesic cues in a word association game.


Lexical choice and social class.

Lexical choice and social class.


The effect of oxprenolol on cardiac intracellular potentials in relation to its anti-arrhythmic, local anaesthetic and other properties.


The social rejection of the alcoholic and the mentally ill in New Zealand.


A cross cultural study of cerebral palsy.


The decision to become a medical student.


A brief description of a study on the utilisation of maternity and child welfare services by a lower working class subculture.


Health knowledge and symptom perception: a study of a rural Kentucky county.


Mental development of children in families and in infants' homes.


A multiphasic health screening of three southern Californian Indian reservations.


Research design for investigation of the lasting behavioural effects of malnutrition: the prblem of controls.


Issues and problems in the teaching of social science in health professional schools.


Simulation by analogue computer of the reaction catalyzed by alcohol dehydrogenase.


Studies on carboxyl modified proteins and their enzymatic hydrolysates.


Effect of ribonuclease on pigeon liver ribosomal ribonucleic acids.


RNase sensitivity of messenger and ribosomal RNA in pigeon liver polysomes.


Effect of diethyl pyrocarbonate on the biological activity of intact TMV and TMV-RNA.


On the dynamics of accommodation for the muscle-response of Anodonta larva and the electric response of Anodonta nerve.


Effect of heavy water on the amino acid incorporation of subcellular liver particles.


Freeze-etching investigation of the ultrastructure of striated myofibrils.


The in vivo absorption of chlorophyll-b in higher plants.


Fatal swelling of mitral ball valve 51 months after replacement.


Histopathologic observations in five cases of congenital tibial pseudarthrosis.


The effect of gynaecologic operations on electrolyte metabolism. I. Effect of abdominal hysterectomy on electrolyte metabolism.


The effect of gynaecologic operations on electrolyte metabolism. II. Effect of adnexal surgery on electrolyte metabolism.


Ophthalmodynamography and ophthalmodynamometry in the aortic-arch and the subclavian-steal syndromes.


The health of diaphragm cell workers exposed to chlorine.


Determination of mercury in urine by atomic absorption.


Monitoring of ozone in air by the peroxy-isocyanate method.


Determination of radon daughters on filters by a simple liquid scintillation technique.


Whole-body counting of some radioactive isotope and radiopharmaceutical production workers.


Effects of exposure of workers to mercury at a sodium hydroxide producing plant.


Current and future training at the university level.


A multi-stage aerosol sampler for extended sampling intervals.


Beta-absorption-impactor aerosol mass monitor.


Particle sampling bias introduced by anisokinetic sampling and deposition within the sampling line.


Carbon monoxide exposures from snow melting machines.


A study of microwave radiation leakage from microwave ovens.


Purification of the coagulation factors VII and X from human serum. Some properties of factor VII.


The in vivo effect of vitamin E in experimental porphyria.


11-deoxysteroid-induced spectral responses in adrenal gland microsomes.


The subunit character of soluble estrogen receptors from rat uteri and their modification in vitro.


The distribution of asparaginase activity in legumes.


Metabolic disposition of [131I] iodoinsulin within the rat diaphragm.


Regulation of the nitrate assimilation pathway of cultured tobacco cells. II. Properties of a variant cell line.


Lack of hydroxylation-induced migration with 4-iodophenylalanine.


Studies on amphibian yolk. IX. Xenopus vitellogenin.


Accumulation of (3',5'-3H2)folic acid by human blood platelets. Effect of dihydrofolic acid reductase inhibitors.


Retention of the alpha-nitrogen in the metabolic conversion of L-lysine to alpha-aminoadipate by the rat.


Studies on methylation and demethylation reactions in isoflavonoid biosynthesis.


Enzymatic ethanolysis of uridine 5'-monophosphate.


Effects of substrates and inhibitors of the tricarboxylic acid cycle on proximal tubular fluid transport in vitro.


A microflow birefringence device.

A microflow birefringence device.


Influence of biliary stasis on alkaline phosphatase in the small intestine.


Substituted iron-chlorins and their complexes with globin. Spectral comparisons with myeloperoxidase.


Studies on a hemagglutinin from the lichen Parmelia michauxiana.


Receptive field properties of directionally selective units in the pigeon's optic tectum.


Afferent impulses in median nerve fascicles evoked by tactile stimuli of the human hand.


Single unit potentials with complex waveform seen in microelectrode recordings from the human median nerve.


Studies on the action of some psychoactive drugs on central noradrenaline neurons after inhibition of dopamine-beta-hydroxylase.


Depletion of catecholamines in vivo induced by electrical stimulation of central monoamine pathways.


Single neurons in the rat medulla responsive to nociceptive stimulation.


Uptake of morphine in particulate fractions from rat brain.


Quantitative recording of rotational behavior in rats after 6-hydroxy-dopamine lesions of the nigrostriatal dopamine system.


A critical evaluation of myelin purification. Non-specific esterase activity associated with central nerve myelin preparations.


Isoelectric focusing of soluble brain proteins and changes associated with development.


Reticular control of motor activity in the cat.


Loss of the ability to perceive the spatial orientation of a rod after posterior right brain damage.


Intracellular study of intralaminar thalmic neurones during brain stem reticular stimulation.


Common aspects of hippocampus electrical activities during different behavioral tasks.


Neural systems involved in intracranial self-stimulation.


Conditioned reinforcement established with intracranial self-stimulation (ICSS).


Effects of rubral area lesions and stimulation on conditioned forelimb flexion responses in the cat.


The sequential organization of grooming behaviour in the rat.


Avoidance learning in the non-compensated functionally decorticate rat.


On the relationship between the deficient performance of hypophysectomized rats in shuttle-box conditioning and the RNA metabolism in their brain stem.


Behavioral changes and brain biochemistry in amphibia.


Frontal and nigral lesions, neurotransmitters and behavioural responses in the rat.


Catecholamines and behaviour.

Catecholamines and behaviour.


The dorsal columns and attention theory.


Structure and function of the archistriatum in the pigeon.


The neural bases of sex differences in nonsexual behavior in animals and man: a review and an evolutionary hypothesis.


Simultaneous recordings from multiple single units in cat visual cortex.


Simple and choice reaction times to lateralized visual stimuli in normal stimuli.


Neuronal correlates of stimulus orientation and retinal motion and their binocular interaction in the visual cortex of chronic cats.


Previous experiences as a factor of successful interocular transfer of visual pattern discrimination in split-brain cats.


Facts and hypotheses concerning memory consolidation processes.


Isolation of meth A cell surface membranes possessing tumor-specific transplantation antigen activity.


A growth-modifying factor from cell lines of human malignant origin.