Beta-alanyl-L-tyrosine. Chemical synthesis, properties and occurrence in larvae of the fleshfly Sarcophaga bullata Parker. 1. A naturally occurring peptide, termed sarcophagine, has been isolated from larvae of the fleshfly Sarcophaga bullata. 2. The physicochemical properties of the natural peptide are identical with those of synthetic beta-alanyl-l-tyrosine. 3. During larval growth the concentration of the dipeptide increases continuously; in the fully grown larva it is the predominant non-protein ninhydrin-positive material. 4. Sarcophagine is rapidly and extensively utilized at the time of hardening and darkening of the insect puparium. 5. By virtue of its much higher solubility than that of free tyrosine, the peptide may serve as a reservoir to provide large amounts of readily available tyrosine for subsequent puparium formation.


Oligonucleotide studies. Optical rotatory dispersion of five homodinucleotides. 1. The optical rotatory dispersion (ORD) of five homodinucleotides, ApAp(3'), CpCp(3'), GpGp(3'), IpIp(3') and UpUp(3') (where A, C, G, I and U represent adenosine, cytidine, guanosine, inosine and uridine respectively, and p to the left of a nucleoside symbol indicates a 5'-phosphate and to the right it indicates a 3'-phosphate), were measured as a function of pH, ionic strength and Mg(2+) concentration. 2. The ORD titrations of ApAp(3') and CpCp(3'), which were made by measuring the ORD curves at closely spaced pH intervals, exhibit a maximum at approx. pH5.0 and 5.7 for ApAp(3') and CpCp(3') respectively in the profile of the magnitude of the first Cotton effect versus pH. The results indicate that the conformational rigidity of these dinucleotides depends on the ionization state of a 3'-terminal phosphate group. 3. ApAp(3') was shown to exist as an approximately 1:1 equilibrium mixture of the two major ionic species represented by Ap((-1))Ap((-1)) and Ap((-1))Ap((-2)) at pH6.16, whereas at pH7.5 it exists exclusively as a form of Ap((-1))Ap((-2)). 4. To ascertain the effects of the presence of a terminal phosphate group and of the ionization of the secondary phosphate on the conformation of adenylate dimer, we measured the ORD of ApA, ApAp(3')CH(3) and ApAp(2'). The rotatory power of the first Cotton effect in the above series of dinucleotides decreased at 20 degrees in the order ApA> ApAp(3')CH(3) approximately ApAp(3')((-1))> ApAp(2') at pH7> ApAp(3') at pH7. 5. The pH-rotation profiles were also obtained for ApAp(2'), CpCp(2') and UpUp(3'), but no corresponding maximum was observed. Although simple nearest-neighbour calculations based on the ORD data of IpIp(3') and 5'-IMP account for the observed ORD spectrum of polyinosinic acid at low salt concentration, there were large discrepancies between calculated and experimental results of the polyguanylic acid ORD even at low ionic strength. 6. The extent to which the amplitude of the Cotton effects of IpIp(3') increases with salt concentration, especially by the addition of Mg(2+), was much greater than that observed for ApAp(3'). The implication of such salt effects on the ORD is considered.


The inhibition by fluorocitrate of rat liver mitochondrial and extramitochondrial aconitate hydratase. 1. The effects of synthetic fluorocitrate were studied on: (a) the oxidation of citrate and cis-aconitate by rat liver mitochondria; (b) the activity of the aconitate hydratase found in the liver cell sap; (c) the activity of the aconitate hydratase solubilized from liver mitochondria. 2. Fluorocitrate was found to be a potent inhibitor of oxidation of citrate but only a weak inhibitor of oxidation of cis-aconitate: 6.7mum-fluorocitrate (containing 4% of the inhibitory isomer) caused 94% inhibition of the oxidation of citrate (2mm) whereas 1.0mm-fluorocitrate was necessary to provoke the same inhibition when cis-aconitate (2mm) was the substrate. The degree of inhibition varied in relation to the respiratory state of mitochondria when fluorocitrate was added. The inhibition could be partially reversed by cis-aconitate. 3. The aconitate hydratase extracted from the mitochondria was much less inhibited by fluorocitrate than was the mitochondria-bound enzyme, and the aconitate hydratase found in the cell sap was even less sensitive. 0.3mm-Fluorocitrate was required to cause 50% inhibition of the reaction citrate-->cis-aconitate, catalysed by the aconitate hydratase extracted from the mitochondria, and 1.2m-fluorocitrate for the extramitochondrial enzyme. For both enzymes the reaction citrate-->cis-aconitate was 2-3 times more sensitive to fluorocitrate than was the reaction isocitrate-->cis-aconitate. The inhibition was of the competitive type for both reactions.


The effect of age on protein synthesis in mouse liver. 1. A system of microsomes and 105000g supernatant from livers of old mice is less able to promote the incorporation of [(14)C]phenylalanine into protein than a similar system from livers of young animals. 2. The decrease in [(14)C]phenylalanine incorporation is attributable to changes in microsomes from old animals rather than in the cell-sap fraction. 3. Decreased synthetic ability is found in various classes of microsomes from older animals, namely unfractionated, light and heavy microsomes, but not in detergent-washed ribonucleoprotein particles. 4. Deletions of certain detergent-soluble microsomal proteins accompany the decreased synthetic ability of microsomes from older animals. 5. Microsomes from old mice are less responsive to a synthetic messenger RNA, polyuridylic acid, and this is partly due to a higher rate of hydrolysis in the presence of cell sap from animals of extreme age. 6. Other more direct evidence, from the priming of a cell-free protein-synthesizing system from bacteria and the examination of ribonucleoprotein particles on sucrose density gradients, suggests that senescence is accompanied by a decrease in messenger RNA content.


Incorporation of tritium from [(4R)-4-3H]mevalonate into abscisic acid.


The action of dilute alkali on bacterial cell walls.


The influence of sex and estrogens on liver kynureninase and kynurenine aminotransferase in the rat.


ADP-D-glucose:alpha-1,4-glucan alpha-4-glucosyltransferase of spinach leaves. Enzymatic synthesis of amylopectin-type polysaccharide in a two-enzyme system.


Studies on the metabolism of the chondroitin 4-sulfate-protein complex of porcine articular cartilage.


Invertebrate connective tissue. I. The amino acid and carbohydrate composition of the collagen from Thyone briareus.


Organ culture of fetal rat pancreas. II. Insulin release induced by amino and organic acids, by hormonal peptides, by cationic alterations of the medium and by other agents.


Biosynthesis of asparagine from beta-L-[4C-15N]cyanoalanine in Lathyrus sylvestris W. seedlings. Origin of the amide nitrogen.


Catabolism of pteridine cofactors. II. A specific pterin deaminase in rat liver.


Intracellular distribution of vitamin K in the rat.


Aggregation of chlorophyll in nonpolar solvents from molecular weight measurements.


The structure of heparin: interaction of heparin with basic dyes by visible spectrometry.


Metabolic studies on renal transport of p-aminohippurate in vitro.


Biosynthesis of streptomycin. V. Origin of the formyl carbon carbon atom of streptose.


[9-14C]Fluorene hydroperoxide as a possible intermediate in the hydroxylation of [9-14C]fluorene by rat liver homogenate.


The activity of rat pancreatic juice enzymes towards naphthol-AS nonanoate.


Uridine diphosphate digitoxose from the leaves of Digitalis purpurea L.


Differential activation by pyridoxal phosphate of aspartate aminotransferase from young and old human erythrocytes.


Induction of liver alkaline phosphatase by bile duct ligation.


Isolation of a mitochondrial 9-S protein with malate dehydrogenase activity and its activation by thyroxine in vitro.


Insulin effect on amino acid transport in bone.


Effect of different carbon sources on the synthesis of lactate dehydrogenase in Mima polymorpha.


The synthesis of cyclic alpha-amino acids. 3. The synthesis of methyl (plus)- and (minus)-3-amino-3-C-carboxy-3-deoxypentopyranosides.


Class differences in the mental illnesses of young hospitalized patients.


Young rebels in a mental hospital.

Young rebels in a mental hospital.


"Gang work" in a psychiatric hospital.

"Gang work" in a psychiatric hospital.


The effect of noradrenaline on the calcium uptake of the sarcoplasmic reticulum.


Screen filtration pressure of blood--an evaluation.


The comparison of the antitomboc action of the thrombin-like fraction of Malayan pit viper venom and heparin.


The effects of oxygen breathing in patients with acute myocardial infarction.


Cardiac rhythm disturbances and the release of catecholamines after acute coronary occlusion in dogs.


A gmetrical model of successive stages in excitation of the human heart; its value as a link between excitation and clinical vectorcardiography.


The frame of reference of three-dimensional vectorcardiograms drawn by a computer-driven cathode ray tube.


Haemodynamics of acute and chronic atrioventricular block in dogs.


Light on light.

Light on light.


The HL-A system and transplantation.

The HL-A system and transplantation.


Nutrition clinics and child-care programs. Conceptual and organizational aspects.


Office electrocardiography in general pediatrics. II. Evolution of the ECG from birth to one year old.


Diphtheria--still lethal. Few younger American physicians have seen a case, but these pediatricians report 95 cases from Southern Iran.


Community health projects in pediatrics. Check list of pitfalls.


Pediatrics in Great Britain. An expository description and some personal views.


Our American Educational System. Do so-called modern schools do more harm than good?


Immunological properties of bovine proinsulin and related fractions.


Some effects of 1-methyl-4-(3-methyl-5-isoxazolyl) pyridinium chloride and phenformin on carbohydrate and lipid metabolism in mice.


Effect of cold (4 degree C.) on catecholamine excretion in the diabetic rat and its relation to autonomic neuropathy.


Complacency in coronary care.

Complacency in coronary care.


Effects of oral and intravenous glucose loading in thyrotoxicosis. Studies of plasma glucose, free fatty acid, plasma insulin and blood pyruvate levels.


Factors affecting mortality in acute myocardial infarction.


The well-read journal.

The well-read journal.


Direct myocardial revascularization with saphenous vein autograft. Clinical experience in 100 cases.


Inhalation lung scanning evaluation--radioaerosol versus radioxenon techniques.


Obliteration of segmental pulmonary artery borders: a method of localizing right lower lobe infiltrates.


Pneumomediastinum.

Pneumomediastinum.


A pathognomonic murmur of "atypical" patent ductus arteriosus.


Relapses after lung resection for tuberculosis.


Reversed reciprocating paroxysmal tachycardia.


Evaluation of oxygen therapy devices by arterial oxygen tensions.


Left-sided pneumoperitoneum and right pneumothorax.


Infectious mononucleosis with pleural effusion.


Sarcoidosis and malakoplakia,.

Sarcoidosis and malakoplakia,.


Georgia radium management project. Introduction.


Measurement of gastroesophageal reflux.

Measurement of gastroesophageal reflux.


Potentiation of gastric acid response in the dog.


Effects of sulfation of taurolithocholic and glycolithocholic acids on their intestinal transport.


Protocollagen proline hydroxylase in normal liver and in hepatic fibrosis.


Studies of malabsorption occurring in patients with Laennec's cirrhosis.


Carbon dioxide tensions in the proximal part of the canine gastrointestinal tract.


Calcium kineticstudies in patients with malaborption syndrome.


Acute hepatic necrosis following an overdose of paracetamol.


Comparative evaluation of motility studies in the diagnosis of esophageal disease.


Effects of hypophysectomy, prolactin, and growth hormone on growth of postmetamorphic frogs.


Neurohypophyseal hormones in amphibia: a comparison of their actions and storage.


Directions in psychiatric treatment of the elderly: role of perspective of the life cycle.


Sex hormone binding proteins. I. Binding of steroids by serum of an elasmobranch (Raja radiata).


What would an administrative evaluation of your record department reveal?


Tooth eruption and menarche of Brazilian-born children of Japanese ancestry.


Sensitivity to gamma irradiation of fungi pathogenic to pears.


Antireflux procedure in surgical correction of long lower ureteral defects.


Bladder smooth muscle excitability and response properties.


Urinary outflow resistance estimation in children. Theory, method, and results.


The urinary excretion of oxalic acid in vitamin B1 deficient rats.


Infection with Trichosomoides crassicauda as a factor in the induction of bladder tumors in rats fed 2-acetylaminofluorene.


Reversible vesical hypertonicity, a consequence of the chronic empty state.


Long term renal vein catheterization.

Long term renal vein catheterization.


Effects of blocking an instrumental avoidance response: facilitated extinction but persistence of "fear".


Alkaptonuric ochronosis.

Alkaptonuric ochronosis.


Investigations on mycoplasma in infectious mononucleosis.


Prevention of disease due to Mycoplasma pneumoniae.


Rhodopseudomonas acidophila, sp. n., a new species of the budding purple nonsulfur bacteria. A succinate-mineral salts medium of pH 5.2 provided selective enrichment conditions for Rhodomicrobium vannielii and for a new species belonging to the Athiorhodaceae, described herein as Rhodopseudomonas acidophila. Seven strains of the new species have been isolated from different sources in the United States and Germany. The cells are rod-shaped or ovoid, 1.0 to 1.3 mum wide and 2 to 5 mum long, and motile by means of polar flagella. Multiplication occurs by budding. The photopigments consist of bacteriochlorophyll a and carotenoids of the spirilloxanthin series, together with new carotenoids. All strains can grow either under anaerobic conditions in the light or under microaerophilic to aerobic conditions in the dark. No growth factors are required. The range of simple organic substrates photo-assimilated resembles that characteristic of Rhodomicrobium. Good photolithotrophic growth is possible at the expense of molecular hydrogen; thiosulfate and sulfide are not utilized.


Subdiaphragmatic stenosis of the inferior vena cava. An experimental investigation of the importance of collateral venous drainage for kidney morphology.


The compromised host and infection. II. Deep fungal infection.


Nephritogenic and rheumatogenic group A streptococci.


Observations on the Folin-Ciocalteu reaction.


Identification of mycobacteria.

Identification of mycobacteria.


A simple chromatographic technique for the detection and confirmation of phenylketonuria.


Low cost automated haematology.

Low cost automated haematology.


Foreskin fibroblast cultures in diagnostic virology.


Factors controlling concomitant immunity in tumor-bearing hamsters: effects of prior splenectomy and tumor removal.


Mechanism by which the spleen may act in regulating tumor immunity after tumor resection.


Electron microscopic study of spinal ganglia from vincristine-treated mice.


Chromosome aberrations induced in rat bone marrow cells by 7,12-dimethylbenz[a]-anthracene.


Effects of oestrogen and progestagen on the composition of human milk.


Pesticide residues in foodstuffs in Great Britain. XI. Further studies of malathion in imported cereals.


A phonological model of children's articulation competence.


A predictive screening test of articulation.


A feedback model of the stuttering problem--an engineer's view.


Innovative school programs: the Oakland schools plan.


Causality in speech pathology.

Causality in speech pathology.


Evaluating speech therapy through precision recording.


Conversational rate control therapy for stuttering.


A portable diagnostic speech audiometry unit.


Behavior change in stuttering through systematic desensitization.


Evidence for the use of occludedforehead bone conduction.


A language development program for a blind language-disordered preschool girl: a case report.


Spastic dysphonia: a case presentation.

Spastic dysphonia: a case presentation.


The timing of surgery in intratemporal facial paralysis.


Facial nerve grafting.

Facial nerve grafting.


The relationship of the facial canal to the annular sulcus.


The effects of local anesthetics on the cochlea of the guinea pig.


The synthesis of DNA and RNA in the developing inner ear.


Renal histologic findings in systemic lupus erythematosus.


General considerations in the treatment of systemic lupus erythematosus.


The World Health Organization and its assembly.


Preliminary studies in the use of 5-fluoro-uracil cream in the treatment of malignant and premalignant skin tumours.


Psychiatric symptoms due to vitamin B12 deficiency without anaemia.


The concentrations of magnesium and potassium in erythrocytes and plasma of geriatric patients with psychiatric disorders.


Epidural analgesia for postoperative pain.


Lingual thyroid: report of a case and review of the literature.


The Melkersson-Rosenthal syndrome.

The Melkersson-Rosenthal syndrome.


An approach to the treatment of varicose veins.


Spleno-renal anastomosis: results in 47 cases.


Cancer of the bladder presenting as "cystitis" or "prostatism".


Phenytoin intoxication in childhood due to incorrect prescribing, dispensing and administration of suspensions.


Anticoagulants in coronary disease.

Anticoagulants in coronary disease.


Acute respiratory disease.

Acute respiratory disease.


Apications of the ultrasonic doppler technique.


Rethinking coronary thrombosis: lessons from experimental therapeutics with anticoagulants.


Alcoholism, drug dependency and sex chromosome abnormalities.


The "YY syndrome".

The "YY syndrome".


Acute renal failure complicating intravenous cholangiography in a patient with Dubin-Johnson syndrome.


Measurement of serum DNA-binding activity in systemic lupus erythematosus.


Legal relation of physician to hospital.


Ulceration and perforation of the nasal septum in systemic lupus erythematosus.


The law and the hospital.

The law and the hospital.


Improving health care.

Improving health care.


Anticoagulant therapy in myocardial infarction.


M. fortuitum not found.

M. fortuitum not found.


Less drug reactions in Jews?

Less drug reactions in Jews?


The practice of pharmacists.

The practice of pharmacists.


Human placental lactogen.

Human placental lactogen.


Non-neutrality of bed rest.

Non-neutrality of bed rest.


Education of the cerebral palsied.

Education of the cerebral palsied.


The epidemiology of sarcoidosis.

The epidemiology of sarcoidosis.


Blunt abdominal trauma treated in an intensive care ward.


Agnogenic myeloid metaplasia: role of splenectomy.


Total fasting, hyperuricaemia and gout.

Total fasting, hyperuricaemia and gout.


Fibula autograft survival following resection of osteoclastoma of radius.


Psoas abscess without spinal disease: the value of lymphangiography in tuberculous lymphadenitis.


Massive oedema and ascites during treatment with anti-depressant drugs.


Seat-belt fracture of the tibia.

Seat-belt fracture of the tibia.


Pregnancy in active chronic hepatitis on immunosuppresse therapy.


Mechanism of deoxycytidinuria in irradiated mice and rats.


The relation of DNA degradation to the repair of radiation injury by L cells.


Deoxypyrimidinuria and lymphocytopenia as indicators of absorbed radiation dose in rats.


Colony-forming units and survival of irradiated mice treated with AET or endotoxin.


Photoelectric reonses of lipid membranes.


The effects of whole-body x-irradiation on mouse liver alpha-hydroxy acid oxidase.


Radiolysis of phenylalanine and tyrosine and aqueous solution.


Inactivation of Rhizopus stolonifer sporangiospores by single and combined treatments of heating, chilling, and gamma-irradiation.


A within-strain difference in radiation sensitivity in the RFM mouse.


Autoradiographic study of the effect of estradiol and irradiation on nucleic acid metabolism of the thymus and lymph node of mice.


tructure and properties of rat thymus deoxyribonucleoprotein: effects of x-irradiation.


Studies on the mechanisms of estradiol-induced radioprotection.


The regulation of pesticides in Italy.

The regulation of pesticides in Italy.


The adrenal cortex and the pregnant diabetic.


The use of behaviour patterns of larval schistosomes in assessing the bilharzia potential of non-endemic areas.


Phosphonomycin: structure and synthesis. Synthesis and resolution of the antibiotic phosphonomycin are described. The structure is (-)(IR, 2S)-1,2-epoxypropylphosphonic acid.


Physiological requirements for world-class performances in endurance running.


Nucleic acid-protein interactions in turnip yellow mosaic virus. Mild alkaline treatment induces the formation of a specific RNA complex inside turnip yellow mosaic virus. Dialysis at pH 4.5 destroys this complex inside the capsid, but does not destroy complexes that have been removed from their capsids. These facts were interpreted in terms of a breakage and subsequent re-formation of RNA-protein links. In combination with supporting potentiometric data, they suggest histidinyl-phosphate salt links or carboxylate-amino hydrogen bonds, or both, as the principal means of association of turnip yellow mosaic virus protein and RNA.


Commitment to sound nutrition.

Commitment to sound nutrition.


The 1969 Nobel prize for physiology or medicine.


Contracture and catecholamines in mammalian myocardium. Contractures induced by KCl are produced in cat ventricular muscle after depletion of catecholamine stores by previous treatment with reserpine or by selective blocking of adrenergic receptor sites with propranalol. Contractures are reproducible for 2 to 3 hours and are markedly depressed by the addition of epinephrine. The relaxation of contracture induced by epinephrine parallels the eftect of this compound of shortening the duration of the normal twitch. The failure of the normal mammalian myocardium to maintain tension in solutions of high KCI concentration appears to be related to the endogenous amounts of catecholamines in the tissue.


Cardiac sympathetic nerve activity: changes induced by ouabain and propranolol. A study of the effects of ouabain and propranolol on the spontaneous activity in the preganglionic sympathetic nerves to the cat heart showed that ouabain can produce both an inhibition and a stimulation of the spontaneous activity in sympathetic nerves. The inhibition appears to be reflex in nature and is not present when the buffer nerves are sectioned. The stimulation is correlated with the development of cardiac arrhythmias and is antagonized by propranolol.


Thyrocalcitonin: evidence for physiological function. A calcium salt given by stomach tube in modest amounts, such as might be ingested in a normal meal, produced hypercalcemia in acutely thyroidectomized fasted rats, whereas in rats with intact thyroid glands the same dose of calcium had little or no detectable effect, presumably because of release of thyrocalcitonin. Thyrocalcitonin apparently protects against hypercalcemia during feeding after deprivation.


Nitrogen enrichment of surface water by absorption of ammonia volatilized from cattle feedlots. Apparatus designed to measure absorption of ammonia from the air by aqueous surfaces was installed near several cattle feedlots and in appropriate control areas. Ammonia absorption rates measured near feedlots were as much as 20 times greater than near the control. Their magnitudes indicate that absorption of ammonia volatilized from cattle feedlots contributes significantly to the nitrogen enrichment of surface water in the vicinity of feedlots.


Electroconvulsive shock effects on a reactivated memory trace: further examination. Rats showed amnesia for conditioned fear training if given an electroconvulsive shock immediately after training. Retention was unimpaired, however, when the electroconvulsive shock treatment was given 1 day after training immediately after the presentation of the stimulus used in the fear conditioning training. These results support the view that electroconvulsive shock disrupts memory trace consolidation but does not disrupt a recently reactivated memory trace.


Central nervous system: recovery of function. Rats with one- or two-stage lesions in the hippocampus, anzygdala, or frontal cortex were tested on a variety of tasks. Even though locus and amount of tissue damage were very similar in the two groups, animals with one-stage lesions were impaired as judged by performance on all problems, while aninmals with two-stage lesionis were not.


Lead poisoning in children: detection by ion-selective electrode.


Physiological effects of audible sound.

Physiological effects of audible sound.


Climateand man.

Climateand man.


Migration and mental illness. Some reconsiderations and suggestions for further analysis.


Relationship of cellular enzyme activity to catgut and collagen suture absorption.


Location of breast carcinoma and prognosis.


Effects of endotoxin on pulmonary capillary permeability, ultrastructure, and surfactant.


Pressure relationships responsible for reflux in patients with hiatal hernia.


Emergency treatment in Ludwig's angina.

Emergency treatment in Ludwig's angina.


Hyperbaric oxygen and regional vasodilatation in pedal ischemia.


Drainage of the massively dilated kidney.


Conservative management of pilonidal sinus.


Renal response to intraoperative fluid administration.


A re-evaluation of lower extremity amputations.


Culdocentesis in the evaluation of blunt abdominal trauma.


Long term venous catheterization in infants.


Role of the humane societies in the history of resuscitation.


Platelet adhesiveness and euglobulin lysis time in dogs given repeated infusion of sodium palmitate.


Evidence for the existence of foetal fibrinogen.


On the in vivo clearance of thrombin.

On the in vivo clearance of thrombin.


The influence of urea on energy metabolism of human blood platelets.


Differences in the effect of antithrombin on thrombin clotting and thrombin esterase activity.


Note on the inactivation of autoprothrombin C with diisopropylfluorophosphate or phenylmethanesulfonyl fluoride.


The human prothrombin-activating enzyme.


Malaria--a military problem.

Malaria--a military problem.


Tissue schizogony of P. v. vinckei.

Tissue schizogony of P. v. vinckei.


Pre-erythrocytic schizonts of one of the original strains of Plasmodium berghei.


Duration of the mature gametocytes of P. gallinaceum.


Lesions in the fine structure of Trypanosoma rhodesiense specifically associated with drug treatment.


The activity of drugs on Trypanosoma congolense in vitro at 37 degrees C.


The effect of antilymphocyte serum and cyclophosphamide on the course of infection of Trypanosoma brucei and T. congolense in rats and mice.


Vascular effects of Trypanosoma brucei infection in the rabbit.


An amoeba-agglutination test with Acanthamoeba (Hartmanella).


Structure and taxonomy of microfilariae.


The distribution of acetylcholinesterase in adult Hymenolepis spp.


Mosquito breeding on Grand Cayman Island in the Britisch Caribbean.


Mosquito cell culture.

Mosquito cell culture.


The maintenance of a small laboratory colony of Glossina and the results of experiments on olfactory responses in tsetse flies.


Some parasites of Simulium larvae.

Some parasites of Simulium larvae.


Some bacterial symbionts of Reduviid bugs.


Toxicological and genetic studies on a strain of bed bugs resistant to organophosphorus insecticides.


The 1964 chikungunya epidemic at Vellore, South India, including observations on concurrent dengue.


Experimental infection of calves with Schistosoma mansoni.


A clinical study of Fasciolopsis buski infection in Thailand.


Multiplication of Trypanosoma brucei and Trypanosoma congolense in vertebrate hosts.


A preliminary note on the separation and infectivity of tissue forms of Trypanosoma brucei.


The first isolates of trypanosomes in Indonesia and in history from primates other than man.


Immunological studies on simian malaria. 3. Immunity to challenge and antigenic variation in P. knowlesi.


Studies on the immunology and serology of leishmaniasis. 8. The identity of strains of Leishmania from Ethiopian diffuse cutaneous leishmaniasis.


Cloxacillin absorption in the Ugandan African.


Filtration of cercariae through microfabric.


Filtration of Entamoeba invadens cysts through microfabric.


A technique for embedding small organisms for electron microscopy.


Schistosoma bovis eggs in human faeces.

Schistosoma bovis eggs in human faeces.


Eggs resembling those of Schistosoma intercalatum in the faeces of a baboon.


Racial differences in Pediculus h. capitis infestation in Guyana.


Toxocara canis infection in a Jamaican child.


Fatalities after treatment of onchocerciasis with diethylcarbamazine.


Chloroquine psychosis.

Chloroquine psychosis.


Polycythemia vera.

Polycythemia vera.


Cerebral circulation time and cerebral blood flow.


Effect of pretreatment with dextran 70 on platelet adhesiveness and thromboembolic complications following percutaneous arterial catheterisation.


Angiographic demonstration of collaterals to the coronary arteries in patients with angina pectoris.


Isotope scanning of spinal cord cysts.

Isotope scanning of spinal cord cysts.


Uptake of 85Sr in osteoarthrosis of the spine in man.


Pulmonary sequestration.

Pulmonary sequestration.


Orbitography with water-soluble contrast media.


Projection of cineroentgen film by a TV system.


Effects of papaverine on gastric secretion in man.


A radiochemical method for monoamine oxidase assay.


New derivatives for electron capture--gas chromatography of steroids: a simplified procedure for measuring plasma testosterone.


Disc electrophoresis of myosin and myosin derivatives in dilute polyacrylamide gels.


Sedimentation coefficients in the zonal ultracentrifuge.


A convenient spectrophotometric assay for monoamine oxidase.


Separation of radiocarbon from radiometals in biological samples.


Determination of phloroglucinol in the presence of phloretin using a modified vanillin reagent.


Separation of glycosaminoglycan saccharide and glycoside mixtures by gel filtration.


Increased output and accelerated analysis time with the automatic amino acid analyzer.


Use of a "self-cleaning" oven to clean liquid scintillation vials and other glassware.


An improved method for enzymic determination of glucose in the presence of maltose.


Identification of biocytin in biotin proteins using high-voltage electrophoresis.


Gas chromatographic determination of isomers of alpha, alpha'-dimethylmuconic acid.


Effects of additive genes at many loci compared with those of a set of alleles at one locus in parent-child and sib correlations.


Population subdivision with respect to multiple alleles.


Placentation and zygosity of twins in Ibadan, Nigeria.


The frequency of twinning in a rural community in Western Nigeria.


On the calculation of human mutation rates from changes in sex ratio.


The relation of measured intelligence to birth order and maternal age.


The investigation of reactive sulphydryls in enzymes and their variants by starch gel electrophoresis. Studies on red cell adenosine deaminase.


Molecular size estimates of human peptidases determined by separate gene loci.


Birth weight of the Bengali new born: effect of the economic position of the mother.


Kinetics of fermentation processes.

Kinetics of fermentation processes.


The isolation of an aliphatic amidase from Pseudomonas aeruginosa.


The kinetics of beta-galactosidase attached to porous cellulose sheets.


Cultivation of yeast on gas oil.

Cultivation of yeast on gas oil.


Microbial transformation of hydrocarbons. II. Growth constants and cell composition of microbial cells derived from n-alkanes.


Composition of cell material and biological value of the cellular protein of a Micrococcus strain grown on hydrocarbons.


Fermentation of 1-hexadecene by Candida lipolytica.


Growth models of cultures with two lipid phases. I. Substrate dissolved in dispersed phase.


Scanning electron microscopy of the bronchus and adnexes of rats.


Urinary excretion of hydroxyproline in leprosy.


An improved method for the determination of homovanillic acid and vanilmandelic acid by gas chromatography.


Enzymatic cleavage of 2-acetamido-1-(beta'-L-aspartamido)-1,2-dideoxy-beta-D-glucose by human plasma and seminal fluid. Failure to detect the heterozygous state for aspartylglycosaminuria.


Lipoprotein lipase activity in patients before and after minor surgical operations.


Automatic determination of urine volume from weight of sample.


A new method for separation and quantitative determination of metabolites of tryptamines in biological material.


A rapid micro diazo technique for measuring total bilirubin.


Column chromatographic separation and fluorometric estimation of metanephrine and normetanephrine in urine.


Normal values and comparison of two spectrophotometric methods for determination of zinc in serum.


Methylmalonyl CoA as an abnormal substrate for delta-aminolevulinic acid synthetase.


The pH optimum for ceruloplasmin oxidase activity in the plasma of several species of animal.


Effects of mannitol on the renal circulation.


The effect of neomycin on bile salt metabolism and fat digestion in man.


Plasma clearance of transcobalamin I- and transcobalamin II-bound Co57 vitamin B 12.


Acetophenetidin-induced methemoglobinemia and its relation to the excretion of diazotizable amines.


A protein inhibitor of erythropoiesis.

A protein inhibitor of erythropoiesis.


Correlations between erythropoietin production and excretion.


Effect of testosterone and of cobalt on erythropoietin production by anephric rats.


A physical explantation of the bimodal distribution obtained by electronic sizing of erythrocytes.


The correlation of fractional excretion of sodium and magnesium in the chronically diseased kidney of dog and man.


Hemolytic effects of standard single dosages of primaquine and chloroquine on G-6-PD-deficient caucasians.


Enzymatic assay for 3,4-dihydroxymandelic acid (DOMA) in human urine, plasma, and tissues.


A simple method for the quantitative determination of serum haptoglobin.


Formal theory of drug dosage regimens. II. The exact plateau effect.


The direct fluorometric assay of conjugated bile acids.


Calculation of the electric structures and spectra for corrin and related compounds.


Asymptotic solution of the Poisson-Boltzmann equation for a charged sphere, and implications.


Investigation of the effect of data error in the analysis of biological tracer data from three compartment systems.


Conductance function of electrolyte transport across lipid films.


The information content of a multistate distribution.


The logarithm in biology. II. Distributions simulating the log-normal.


Ionic linkages in protein interactions.

Ionic linkages in protein interactions.


Psycho-logics. An axiomatic system describing human behav- ior.


The unwinding of the DNA molecule during replication.


Comments by the authors on the paper "Thermodynamic aspect of Developmental Biology".


An analysis of experimental foreign body stone formation.


The surgeon's responsibility.

The surgeon's responsibility.


Urbanization and mental health.

Urbanization and mental health.


The mystery of the water factor.

The mystery of the water factor.


The risk of premarital conception.

The risk of premarital conception.


Primary hypomagnesaemia with secondary hypocalcaemia in an infant.


Medical aid to vietnam.

Medical aid to vietnam.


The economics of illness. An employee sickness study.


Cardiac resuscitation: a review of methods and assessment of results.


Rheumatic fever in Western Australia. An analysis of 287 cases, 1957 to 1967.


A preliminary survey of 24 Victorian marijuana users.


Gastric and duodenal ulcer. The association between aspirin ingestion, smoking and family history of ulcer.


Drug referral centre.

Drug referral centre.


Gaucher's disease: report of a case.

Gaucher's disease: report of a case.


A profile of drug takers.

A profile of drug takers.


A case of severe schistosomiasis (Schistosoma mansoni) in Western Australia.


Patient transportation.

Patient transportation.


The home-concept of psychotic patients and the prevention of chronic hospitalization.


Contributions to the morphophysiological study of the auditory apparatus. When does the pneumatization of cephalic cavities appear? When does hearing appear in humans?


The danger of an inadequate water intake during marathon running.


Hepatic and extrahepatic steroid metabolism in extracorporeal circulation with and without hypothermia in dogs.


Upper limb-cardiovascular syndrome.

Upper limb-cardiovascular syndrome.


My experience as a health educator in a Bantu tuberculosis hospital.


Additional medical schools.

Additional medical schools.


The determination of body surface area.

The determination of body surface area.


Metabolism of 4-hydroxy fatty acids by rat liver slices.


The incorporation of glycine-2-14C into urinary uric acid in normal and porphyric human subjects.


Studies on human platelets. VI. Study of platelet abnormalities by density gradient separation of platelet populations.


Rugby injuries.

Rugby injuries.


Biogenetic type syntheses of aporphine alkaloids, isoboldine and glaucine.


The neutral triterpenes of the bark of Picea sitchensis (Sitka spruce).


Ponasteroside A, a glycoside of insect metamorphosing substance from Pteridium aquilinum var. latiusculum: structure and absolute configuration.


Phenothiaphosphines.

Phenothiaphosphines.


Implantation of homologous and heterologous aortic valves in prosthetic vascular tubes.


Radiology of homograft aortic valves.

Radiology of homograft aortic valves.


Cardiac ventricular aneurysm.

Cardiac ventricular aneurysm.


Left ventricular aneurysm.

Left ventricular aneurysm.


A comparison of the quantitative anatomy of the  bronchi in normal subjects, in status asthmaticus, in chronic bronchitis, and in emphysema.


Acomparison of changes in the mucous glands and goblet cells of nasal, sinus, and bronchial mucosa.


Electron microscopy of desquamative interstitial pneumonia.


Association between radiological findings and total and regional function in emphysema.


Effect of aerosol propellants and surfactants on airway resistance.


Bronchopulmonary anastomoses in sickle-cell anaemia.


Surgical management of tricuspid atresia.


Injuries of the heart and great vessels due to pins and needles.


Tuberculosis of the oesophagus.

Tuberculosis of the oesophagus.


A review on human leukemias following radiotherapy in Japan.


Limy bile and porcelain gallbladder. Report of our cases and review of Japanese literature.


Studies on ixodid ticks. X. Haemaphysalis megaspinosa n. sp. (Ixodoidea, Ixodidae) from Kanagawa Prefecture, Japan.


Cortisone-induced nephropathy in young and adult rabbits.


Immunology of cancer.

Immunology of cancer.


The paying patient and surgical teaching.


Problems in the management of battlefield vascular injuries.


Popliteal artery injuries in Vietnam.

Popliteal artery injuries in Vietnam.


Reduction of hernia "en masse".

Reduction of hernia "en masse".


Pericardial tamponade due to penetrating fragment wounds of the heart.


Organization of medical services under group practice with prepayment.


Bowel obstruction in the aged patient. A review of 300 cases.


Bilateral carcinoma of the breast.

Bilateral carcinoma of the breast.


Retroperitoneal hemorrhage secondary to blunt trauma.


Mediastinoscopy. An important adjunct in the diagnosis and treatment of intrathoracic lesions.


Intraperitoneal gastric fixation of hiatal hernia.


Hydatid cyst. Occurrence in central California.


In support of blind tube cecostomy in acute obstruction of the descending colon. Analysis of ninety-three emergency cecostomies.


Routine operative cholangiography.

Routine operative cholangiography.


Ischemic necrosis of the proximal gastric remnant. A complication of subtotal gastrectomy.


Surgical treatment of peptic ulcer.

Surgical treatment of peptic ulcer.


Intussusception of a fallopian tube.

Intussusception of a fallopian tube.


Repair of recurrent inguinal hernia.

Repair of recurrent inguinal hernia.


Scalene lymph node dissection. A study of 354 consecutive dissections.


Scleroderma with diverticulosis and colonic obstruction.


Trauma. Every surgeon's concern.

Trauma. Every surgeon's concern.


Hemostatic risk. Preoperative assessment.


Necrotizing colitis.

Necrotizing colitis.


Replantation of almost completely amputated thigh with recovery.


Phlebography of the lower extremity and pelvic region.


Drainage of low anterior anastomoses.

Drainage of low anterior anastomoses.


Spontaneous rupture of the kidney with massive hemorrhage. A complication of anticoagulation.


The neglected hiatus hernia. Late complications of peptic esophagitis: esophageal stenosis and shortening.


Radical pancreatoduodenectomy for periampullary malignancy.


Studies on the mechanism of activation and inactivation of pyruvate, phosphate dikinase. A possible regulatory role for the enzyme in the C4 dicarboxylic acid pathway of photosynthesis. 1. The activity of pyruvate,P(i) dikinase in leaves of maize and Amaranthus palmeri rapidly falls on transferring illuminated plants to darkness. Illumination of dark-treated plants results in an immediate rapid increase in activity of the enzyme, the final activity reached being dependent on the intensity of the incident light. 2. Activation of the enzyme in extracts of dark-treated maize leaves after gel filtration on Sephadex G-25 requires a thiol and P(i). The P(i) requirement for activation can be replaced by arsenate. Activation of the enzyme is inhibited by AMP and GMP and possibly also by ADP and ATP. Activation of the enzyme after gel filtration on Sephadex G-200 also requires a heat-labile component that is excluded by Sephadex G-25. 3. The active enzyme isolated from illuminated leaves is inactivated by ADP in the presence of a thiol, the rate of inactivation being very much faster in air than in an oxygen-free atmosphere. Reactivation of the ADP-inactivated enzyme requires a thiol, P(i) and a component excluded by Sephadex G-25 but considerably retarded by Sephadex G-200. 4. The active enzyme is rapidly and irreversibly inactivated in the absence of a thiol. Inactivation is accelerated by both sodium diethyldithiocarbamate and tetraethylthiuram disulphide, and the enzyme inactivated by these reagents is completely reactivated by incubation with dithiothreitol. This reactivation does not require P(i). The inactive enzyme from dark-treated leaves is stabilized by diethyldithiocarbamate and can be partially activated by dithiothreitol alone; complete reactivation requires both dithiothreitol and P(i). 5. The enzyme activity is markedly inhibited by the thiol reagents p-chloromercuribenzoate, gamma-(p-arsenophenyl)-n-butyrate and an equimolar mixture of arsenite and 2,3-dimercaptopropan-1-ol. 6. The processes of activation and inactivation observed in vitro are discussed in relation to the regulation of pyruvate,P(i) dikinase activity in the leaf.


Evidence for the presence of several lipases n cow's milk. Skim milks containing sodium chloride (0.75m) were centrifuged at 80000g for 2hr. and portions of the supernatants were submitted to gel filtration on columns of Sephadex G-200. Enzymes in the effluent fractions were assayed titrimetrically for their hydrolytic activities towards tributyrin, triolein and milk-fat emulsions, and triacetin solution. Summation of the measurements gave ratios of activities towards the various substrates similar to those of the original skim milks. Although only partial separation was obtained, five enzymes appeared to be present. They showed some differences in substrate specificity, but all appeared to be lipases in that they hydrolysed the emulsified substrates more rapidly than the dissolved triacetin.


The reduction of cystine during its transpot into rat jejunal eveted segments and sacs. 1. Everted segments and sacs of rat jejunum were incubated in buffer containing [(35)S]cystine. 2. Concentration gradients were achieved by both segments and sacs, and the effects of duration of incubation and of cystine concentration on the isotope distribution ratios were determined. 3. Kinetic constants were determined for the uptake of cystine by both segments and sacs, and the differences between the two systems are discussed. 4. Reduction to cysteine was virtually complete intracellularly and in the sac lumen. Extensive reduction in the medium occurred only when segments were incubated. 5. Anaerobiosis prevented a concentration gradient being obtained between the medium and the tissue, but had little effect on the extent of reduction to cysteine in the tissue and sac lumen. 6. It is concluded that cystine is transported by an active process into rat jejunum, where it is present almost entirely in the reduced form, and that efflux of cysteine occurs through the serosal surface.


Nuclear-magnetic-resonance spectrum of a sulphone derivative of chlorogenoquinone.


The separation, prtial purificatio nd some properties of isoenzymes of aldolase from guinea-pig cerebral cortex. 1. Aldolase isoenzymes from guinea-pig cerebral cortex were partially purified and separated by ammonium sulphate fractionation and chromatography on DEAE-cellulose. 2. Each purified isoenzyme was shown to be virtually uncontaminated with other forms by starch-gel electrophoresis. The quantitative distribution of the isoenzymes was: I, 6.2%; II, 5.2%; III, 15.3%; IV, 25.7%; V, 33.3%. 3. The pH optima for the five separated isoenzymes were similar; all were in the range pH7.5-8.0. Values for pK(a) (6.31-6.55) and pK(b) (9.45-9.59) were calculated from the data and suggested the involvement of histidine and lysine residues. 4. The stabilities of the isoenzymes were shown to be I=II>III>IV>V at pH4.4 in order of decreasing stability and are discussed in terms of subunit structure. 5. The substrate activity ratios (fructose 1,6-diphosphate/fructose 1-phosphate) were measured and all were in the range 12-44.


Utilization of L-threonine by a species of Arthrobacter. A novel catabolic role for "aminoacetone synthase". 1. A species of Arthrobacter (designated Arthrobacter 9759) was isolated from soil by its ability to grow aerobically on l-threonine as sole source of carbon atoms, nitrogen atoms and energy; the organism also grew well on other sources of carbon atoms including glycine, but no growth was obtainable on aminoacetone or dl-1-aminopropan-2-ol. 2. During growth on threonine, (14)C from l-[U-(14)C]threonine was rapidly incorporated into glycine and citrate, and thereafter into serine, alanine, aspartate and glutamate. 3. With extracts of threonine-grown cells supplied with l-[U-(14)C]threonine, evidence was obtained of the NAD and CoA-dependent catabolism of l-threonine to produce acetyl-CoA plus glycine. Short-term incorporation studies in which [2-(14)C]acetate and [2-(14)C]glycine were supplied (a) to cultures growing on threonine, and (b) to extracts of threonine-grown cells, showed that the acetyl-CoA was metabolized via the tricarboxylic acid cycle and glyoxylate cycle whereas the glycine was converted into pyruvate via the folate-dependent ;serine pathway'. 4. The threonine-grown organism contained ;biosynthetic' threonine dehydratase and a potent NAD-linked l-threonine dehydrogenase but possessed no l-threonine aldolase activity. 5. Evidence was obtained that the acetyl-CoA and glycine produced from l-threonine had their immediate origin in the alpha-amino-beta-oxobutyrate formed by the threonine dehydrogenase; the CoA-dependent cleavage of this compound was catalysed by an alpha-amino-beta-oxobutyrate CoA-ligase, which was identified with ;aminoacetone synthase'. A continuous spectrophotometric assay of this enzyme was developed, and it was found to be inducibly synthesized only during growth on threonine and not during growth on acetate plus glycine. 6. By using a reconstituted mixture of separately purified l-threonine dehydrogenase and alpha-amino-beta-oxobutyrate CoA-ligase (i.e. ;aminoacetone synthase'), l-[U-(14)C]threonine was broken down to [(14)C]glycine plus [(14)C]acetyl-CoA (trapped as [(14)C]citrate). 7. There was no evidence of aminoacetone metabolism by Arthrobacter 9759 even though a small amount of this amino ketone appeared in the culture medium during growth on threonine.


Electrophoretic differentiaion f myofibrillar proteins in the pig. 1. Starch-gel electrophoretograms of myosin and tropomyosin preparations in 8m-urea, from longissimus dorsi and psoas muscles of the pig, were characterized by laser densitometry. 2. The typical pattern for freshly prepared myosin from both muscles was similar, there being five electrophoretically distinct components. 3. The number of electrophoretically distinct components in both muscles increased after freeze-drying, but the effect of freeze-drying was more marked in longissimus dorsi. 4. Extraction with 8m-urea containing 2% beta-mercaptoethanol decreased the number of major electrophoretically distinct components of the fresh myosin of both muscles to four. 5. Although there was also some simplification of the patterns after freeze-drying the greater susceptibility of the myosin from longissimus dorsi was still evident. 6. The typical pattern for freshly prepared tropomyosin in 8m-urea differed in the two muscles: in each case it was more complex than that of the corresponding myosins. 7. The pattern of tropomyosin from neither longissimus dorsi nor psoas was altered significantly after freeze-drying. 8. Electrophoretograms of pig longissimus dorsi tropomyosin in 8m-urea differed from those of longissimus dorsi tropomyosin from sheep, ox and rabbit. 9. Extraction of the tropomyosins in 8m-urea and 2% beta-mercaptoethanol simplified the electrophoretic pattern to two major components with samples from pig, sheep and ox, and to one major component with samples from rabbit. 10. It was concluded that classification of skeletal muscles as ;red' or ;white' is insufficient to account for the degree of functional specialization which the electrophoretograms suggest.


The use of maleic anhydride for the reversible blocking of amino groups in polypeptide chains. 1. Maleic anhydride was shown to react rapidly and specifically with amino groups of proteins and peptides. Complete substitution of chymotrypsinogen was achieved under mild conditions and the extent of reaction could be readily determined from the spectrum of the maleyl-protein. 2. Maleyl-proteins are generally soluble and disaggregated at neutral pH. Trypsin splits the blocked proteins only at arginine residues and there is frequently selectivity in this cleavage, e.g. in yeast alcohol dehydrogenase and pig glyceraldehyde 3-phosphate dehydrogenase. 3. The group is removed by intramolecular catalysis at acid pH. The half-time was 11-12hr. at 37 degrees at pH3.5 in in-maleyl-lysine or in maleyl-chymotrypsinogen. 4. The unblocking reaction can be used as the basis for a ;diagonal'-electrophoretic separation of lysine peptides and N-terminal peptides, as shown by studies with beta-melanocyte-stimulating hormone.


Experimental conditions affecting ribonucleic acid polymerase in isolated rat liver nuclei. Effect of nucleoside triphosphate concentration, temperature, ammonium sulphate and heparin. 1. The conditions affecting the activity of RNA polymerase in isolated rat liver nuclei were studied with Mg(2+) or Mn(2+) as activating ions. 2. The enzyme assayed with Mg(2+) and at low ionic strength is saturated by a lower concentration of nucleotide substrates than if assayed with Mn(2+) at low ionic strength or with either ion at high ionic strength. 3. At low and at high ionic strength the incorporation of AMP is affected in a similar way by variations in the temperature of incubation. Preincubation at 37 degrees impairs the AMP incorporation. 4. Heparin stimulates the RNA polymerase activity in the presence of Mn(2+). 5. Both ammonium sulphate and heparin ;restart' the reaction if added after 15min., the effect being more marked with ammonium sulphate than with heparin, and also more marked in the presence of Mn(2+) than of Mg(2+). 6. alpha-Amanitin abolishes the effect of ammonium sulphate and of heparin.


Comparative studies of bile salts. 16-Deoxymyxinol, a second bile alcohol from hagfish. 1. Material containing the less polar sulphate previously noticed in hagfish bile salts gave, after dioxan-trichloroacetic acid cleavage, 16-deoxymyxinol [3beta,7alpha,-26(27)-trihydroxy-5alpha-cholestane]. 2. Anodic coupling of 3beta-hydroxy-5beta-cholanoic acid and the mixed half esters of dl-methylsuccinic acid, followed by lithium aluminium hydride reduction, yielded 3beta,26(27)-dihydroxy-5beta-cholestane. 3. 16-Deoxymyxinol, the third known bile alcohol having the 3beta-hydroxy-5alpha-hydrogen configuration, poses again the question of how the 3beta-hydroxyl group of cholesterol can be ;retained' in biosynthesis of primitive bile salts.


Biosynthesis of thyroglobulin. Incorporation of [1-14C] galactose, [1-14C] manose and [4,5-3H2] leucine into soluble proteins by rat thyroids in vitro. 1. Rat thyroid lobes were incubated for various periods of time in Krebs-Ringer bicarbonate containing [(3)H]leucine and either [1-(14)C]galactose or [1-(14)C]mannose. Radioactivity in soluble proteins was determined after their separation by sucrose-gradient centrifugation. 2. The time-course of incorporation of label from [(14)C]-mannose into soluble thyroid proteins was parallel to that observed for [(3)H]leucine. There was a lag of at least 30min. before either label appeared in non-iodinated thyroglobulin (protein 17-18s). During this time both labels were detected in two fractions known to contain subunit precursors of thyroglobulin (fractions 12s and 3-8s). Radioactivity from double-labelled fractions 12s and 3-8s was transferred to protein 17-18s during subsequent incubation in an unlabelled medium. 3. In contrast, most of the [(14)C]galactose was immediately incorporated into protein 17-18s. 4. During the first hour of incubation, puromycin almost completely inhibited the incorporation of label from [(3)H]leucine and [(14)C]mannose into all protein fractions, but had little effect on the incorporation of [(14)C]galactose into protein 17-18s. 5. These results indicate that mannose is incorporated into the carbohydrate groups of protein 17-18s at an earlier stage in its formation than galactose. It is suggested that the synthesis of the carbohydrate groups of ghyroglobulin begins soon after formation of the polypeptide components, more than 30min. before these are aggregated to protein 17-18s; carbohydrate synthesis then proceeds in a stepwise manner, galactose being incorporated at about the time of aggregation of subunits to protein 17-18s. Most, if not all, the carbohydrate is added to thyroglobulin before it is iodinated.


Nuclear-magnetic-resonance and mass-spectral study of 16-deoxymyxinol.


The structure of an acylated inositol mannoside in the lipids of propionic acid bacteria. 1. Lipids were extracted from five strains of Propionibacterium with chloroform-methanol mixtures and fractionated by chromatography on silicic acid. 2. All five extracts contained a glycolipid composed of fatty acids, inositol and mannose in the molar proportions 2:1:1. 3. Hydrolysis of the glycolipid with alkali gave a mixture of fatty acids and O-alpha-d-mannopyranosyl-(1-->2)-myoinositol. 4. Analysis of the fatty acids by g.l.c. showed that they were predominantly straight- and branched-chain isomers of pentadecanoic acid and heptadecanoic acid. 5. The location and distribution of the fatty acid residues in the molecule was established by periodate oxidation studies and mass spectrometry. The structure of the major glycolipid is 1-O-pentadecanoyl-2-O-(6-O-heptadecanoyl-alpha-d-mannopyranosyl)myoinositol. 6. The glycolipids are located in the membrane; the cell walls are devoid of lipid. 7. Possible functions of the glycolipid are discussed.


A shift in the optimum pH of pospholipase D produced by activating long-chain anions. 1. The activity of phospholipase D (phosphatidylcholine phosphatidohydrolase, EC 3.1.4.4) towards ultrasonically treated phosphatidylcholine or large phosphatidylcholine particles activated with ether was maximal near pH5, and there was little activity above pH6. 2. When the enzyme was activated by the addition of phosphatidic acid to large phosphatidylcholine particles the pH optimum was shifted to pH6.5 irrespective of the amount of activator added. 3. When the enzyme was activated with low concentrations of dodecyl sulphate the pH optimum was 5.5 with little activity above pH6. With higher concentrations of dodecyl sulphate the pH-activity profile was shifted upwards towards a pH optimum of 6.5-6.6, the magnitude of the shift depending on the extent of the hydrolysis. 4. The shifts in the pH-activity profiles cannot be correlated with changes in the ;surface pH' of the substrate particles calculated from the measurement of their zeta-potentials (electrophoretic mobilities).


Kinetic complexity of chloroplastal deoxyribonucleic acid and mitochondrial deoxyribonucleic acid from higher plants. 1. Chloroplasts and mitochondria were isolated by aqueous and non-aqueous cell-fractionation techniques. In a variety of higher plants the mitochondrial DNA bands in a caesium chloride gradient at 1.706g.cm.(-3), whereas chloroplastal DNA has a buoyant density of 1.697g.cm.(-3). 2. In total cellular DNA of moderate molecular weight, the chloroplastal DNA is found within the Gaussian distribution of the nuclear DNA and is not resolved as a satellite. 3. Both chloroplastal DNA and mitochondrial DNA from lettuce renature rapidly. 4. The kinetic complexity of mitochondrial DNA is > 10(8) daltons. 5. Chloroplastal DNA is made up from fast and slow renaturing sequences with kinetic complexities of 3x10(6) and 1.2x10(8) daltons respectively. 6. From the discrepancy between analytical and kinetic complexity it is concluded that chloroplastal DNA is extensively reiterated.


The non-involvement of cyl-carrir protein in poly-beta-hydroxybutyric acid biosynthesis in Azotobacter beijerinckii.


Impaired pentose phosphate shunt and decreased glycolytic activity in lymphocytes of chronic lymphocytic leukemia. Metabolic pathway.


Delayed clearance of chloramphenicol from serum in patients with hematologic toxicity.


Studies in iron absorption. V. Effect of gastrointestinal factors on iron absorption.


Durable normal erythropoiesis in organotypic cultures of guinea pig foetal liver.


Studies in iron absorption. IV. Effect of humoral factors on iron absorption.


Studies in iron absorption VI. Transitory effect of oral administration of iron on iron absorption.


Other malignant neoplasms associated with carcinoma of the thyroid: thyroid carcinoma multiplex.


Epidemiologic investigation of multiple primary cancer of the upper alimentary and respiratory tracts. I. A retrospective study.


Study of intraglandular dissemination of thyroid cancer.


Primary malignant lymphoma of the urinary bladder.


Relationship of squamous cell carcinoma of the cervix uteri to squamous cell carcinoma of the penis among Puerto rican women married to men with penile carcinoma.


Beta-glucuronidase activity in adrenals of patients with or without cancer.


Solitary amyloid mass of the lung. Report of a case with 6-year follow-up.


A standard medical history form.

A standard medical history form.


Biological activities of drugs. VI. Structure-activity relationship of sulfonamide carbonic anhydrase inhibitors. (1).


Studies of nucleosides and nucleotides. XXXIX. Synthesis of 8-substituted purine nucleotides by the direct replacement reactions.


Studies on bile-sensitive lipase. V. Purification and properties of lipase from Mucor javanicus.


Studies on bile-sensitive lipase. VI. Effect of bile salts on Mucor lipase.


Studies on application of amino acid as medicinal agent. II. Reaction of amino acid ester with difunctional Grignard reagent.


Inhibition and stimulation of the biosynthesis of protein and nucleic acid. IV. Effect of 2-amino-1,4-naphthoquinone imine on the biosynthesis of nucleic acid and protein in Ehrlich mouse ascites tumor cells in vitro.


Studies on the glucaric acid pathway in the metabolism of D-glucuronic acid in mammals. II. Excretion of D-glucaric acid in urine after administration of several monosaccharides and their derivatives to mammals.


Studies on microcapsules. 3. Permeability of polyurethane microcapsule membranes.


Actual microanalysis by large volume injection to gas chromatograph equipped with electron capture detector.


Stereostructure of rhodojaponin I, II, and 3, toxins of Rhododendron japonicum, and of asebotoxin 3, toxin of Pieris japonica.


Optical properties of thiopyrimidine nucleosides.


Harderian gland. IV. Porphyrin formation from delta-aminolevulinic acid by the Harderian gland of rats.


Studies on antitumor substances. X. Reactions of thiosulfonates with some nucleophilic compounds.


Absorption of solute from the solutions. 2. Repression of adsorption of cyanocobalamin on talc by polyvinylpyrrolidone.


Reaction mechanism in aromatic heterocyclic compounds. X. Kinetics of the reaction of carcinogenic 4-nitroquinoline 1-oxide and its derivatives with sodium ethoxide.


Drug-induced ultrastructural alterations of striated muscles.


Genetical relationship and isolating mechanisms in Ananassae species subgroup (Sophophora, Drosophila). 3. Chromosome behaviour during meiosis in D. bipectinata, D. malerkotliana and their hybid.


Mitotic cell studies based on in vivo observations. I. Differences of mitotic figures between living cells and fixed cells.


Half-chromatids in the human chromosomes from leucocyte cultures.


A study of mitotic index in differentiating central nervous system of chick.


Cytological and autoradiographic study on ribonucleic acid synthesis in preimplantation eggs of the Syrian hamster.


Opening medical education.

Opening medical education.


Reevaluation of the treatment of shock secondary to cardiac infarction.


Resection of postmyocardial infarction ventricular aneurysms for cardiac arrhythmias.


Efficacy of atropine in the treatment of digitalis induced A-V block.


Glossary of cardiologic terms related to physical diagnosis and history. 3. Anterior chest movements: the ventricles, the atria, and the great vessels.


Competence and electrophoretic spectrum of the cells dissociated from Triturus gastrula ectoderm.


An analysis of the cytotoxic effect of antibodies upon embryonic cells: a study on chicken embryonic metanephros.


Changes in the isozyme patterns of lactate dehydrogenase (LDH) in the in vitro cultured muscle cells.


Intracellular position of nuclear division in protonema of Pteris vittata.


Experimentally induced apical dominance in protonemata of Pteris Vittata.


Cell cycle in the primitive streak and the notochord of early chick embryos.


Cell cycle in the primary embryonic induction of chick embryos.


Effects of amphetamine and desmethylimipramine on amines synthesis and release in central catecholamine-containing neurons.


Modification by metaraminol of reserpine action on noradrenaline stores in the rat heart.


Interaction of the mitotic inhibitor 3-hydroxy-6-methylpyrid-2-thion with DNA.


Are adrenergic nerves required for uptake in the isolated perfused rat heart?


The effect of thujaplicin (4-isopropyl tropolone) upon the general and cardiac haemodynamics and metabolism of the intact dog.


Storage of exogenous monoamines and reserpine in 5-hydroxytryptamine organelles of blood platelets.


Endocrine influence on urinary 5-hydroxyindoleacetic acid output in the rat.


Decreased turnover in central 5-HT nerve terminals induced by antidepressant drugs of the imipramine type.


Effects of a sulfhydry reagent, N-ethylmaleimide, on electrical and mechanical activities of isolated atria.


Some effects of prolonged treatment with atropine on the stomach wall of the rat (a biochemical-pharmacological approach of the effects of vagal exclusion--vagotomy and atropine treatment--on cellular-molecular level).


Base alterations of nucleic acids in stomach wall after prolonged atropine treatment.


Inhibition of steroid delta-4-reductase by polysulfated polysaccharides and para-chloromercuribenzoic acid.


Streptozotocin- and alloxan-diabetes in mice.


Formation of ferrihaemoglobin with aminophenols in the human for the treatment of cyanide poisoning.


An electron microscope study of the basement membrane of proliferated bile ductules.


Ultrastructural pathology of murine pneumonitis caused by K-Papovavirus.


Ultrastructural study on the ribosome helix and its change after antigenic stimulation in P3-J cells.


The effect of nicotine on dietary atherogenesis in rabbits.


Tissue repair in presence of locally applied inhibitors of fibrinolysis.


Metachromasia induced by inositol-hexa-phosphate with toluidine blue.


The in vivo incorporation of labelled acetate into the cholesterol and fatty acids of tissues of dystrophic mice.


Histochemical demonstration of L-amino acid-tetrazolium reductase.


Dental pulp pH under silicate cement and calcium hydroxide dressings.


Neuroaxonal dystrophy: its relation to age and central nervous system lesions.


Identification of type specific streptococcal antibodies by in vitro phagocytosis.


Changes in active phosphorylase activity and glycogen content during larval and pupal development of Philosamia ricini.


On the problem of thromboemboli related to heart valve prosthesis: a survey of 139 consecutive cases.


Solubilized RNA polymerase from rat liver nucleoli.


A survey of bacterial endocarditis during a 12-year period.


Inhibition by cholesterol analogues of the side-chain cleavage of cholesterol and 20alpha-hydroxycholesterol in a preparation of hog adrenocortical mitochondria.


Reversal of excessive hypertension in patients with terminal renal failure by intermittent hemodialysis and bilateral nephrectomy.


Amino sugar analysis by gas-liquid chromatography.


Studies on the substrate specificity of Taka-amylase A. II. The mode of action of Taka-amylase A on O-methyl derivatives of phenyl alpha-maltoside.


Colorimetric determination of omega-amino acids.


Abdominal aortic dissection presenting as femoral artery occlusion.


Relation of kidneys and adrenal glands to hypovolemic thirst.


The effect of chain length of substrate on the formation of mono-unsaturated fatty acids in rat liver preparation.


Demand coupled pacing.

Demand coupled pacing.


Descending nerve tracts in the spinal cord of the rat. I. Fibers from the midbrain.


Effect of a single dose of phentolamine and MJ 1999 on indomethacin-induced gastric ulcer in rats.


A light and electron microscopic study of the normal and the degenerating corticospinal tract in the rat.


Diminished functional parathyroid reserve following I-131 treatment for hyperthyroidism.


Electron microscopic features and proliferation of astrocytes in the corpus callosum of the rat.


Isolation of amoebae from the air.

Isolation of amoebae from the air.


Work of WHO in Africa.

Work of WHO in Africa.


Chondroitin sulphate in treatment of experimental bone defects.


The toxins responsible for the lesions of Clostridium sordelli gas gangrene.


Cytopathic effects of pathogenic and non-pathogenic species of Candida on cultured mouse epithelial cells: relation to the growth rate and morphology of the fungi.


The increase of hemoglobin A2 to its adult level.


A proposal for the establishment of two new Haemophilus species.


Letter to the editor: Schistosoma haematobium around Lake Tana.


Sensitivity of the genus Proteus to chlorhexidine.


Laboratory diagnosis of epidemic keratoconjunctivitis.


The effect of antilymphocyte serum on subcutaneous staphylococcal infections in normal, immune and complement-deficient mice.


Composition of cell walls of Clostridium sordelli and Clostridium bifermentans and its relation to taxonomy.


Structure of natural cognitions.

Structure of natural cognitions.


Toward an attribution therapy: the reduction of fear through induced cognitive-emotional misattribution.


Obesity, food deprivation, and supermarket shopping behavior.


Acting on an attitude as a function of self-percept and inequity.


Zeigarnik effect, "reversed Zeigarnik effect", and personality.


Identification: the acquisition of evaluative connotations.


Early changes in the forearm circulation following transient increase of local external pressure. 1. The pressure at the surface of a segment of forearm enclosed in a plethysmograph was abruptly raised from atmospheric level to +40 mm Hg, held at the new level for 4 sec, and abruptly dropped to atmospheric level.2. Forearm circumference (V(f)) equivalent to the volume of a small segment of forearm, was monitored with a mercury-in-rubber strain gauge. Pressure was measured in the cylinder (P(p)) in veins exposed to external compression (P(v, e)), and in the radial artery exposed to compression (P(ra)).3. Forearm blood flow was measured by venous occlusion plethysmography before, and after, release of external compression. There was, on average, over the 3rd and 4th second after release of pressure, a 2.4-fold increase of inflow, as compared with resting level.4. By the 15th second after release of compression, forearm blood flow had returned to its previous resting level.5. The increase in blood flow after compression appears to be due to active reduction in vascular resistance, for refilling of the arteries and arterioles would be completed before the increased flow was recorded; venous backflow can be excluded, and the pressure difference for flow (arterial minus venous) is virtually unchanged.


Beta-D-glucuronidases and the functional activity of the mammalian nephron.


Post-tetanic potentiation at the neuromuscular junction of the frog. 1. Post-tetanic potentiation (PTP) was studied at the neuromuscular junction of the frog. The magnitude and time course of PTP was dependent on the number of stimuli in the tetanus, rather than on the frequency or duration of the tetanus. At low temperature the maximum amplitude of PTP was unchanged, but the time course was prolonged.2. The magnitude and time course of PTP was accounted for quantitatively by estimated changes in the fraction of transmitter released per stimulus.3. As external [Ca] was reduced, the time for decay of PTP was decreased. The maximum amplitude of PTP, measured with respect to control amplitude at the same [Ca], was unchanged, but occurred at earlier times. The time course of PTP was dependent only on the [Ca] present during the tetanus.4. It is concluded that PTP is associated with an intracellular accumulation of Ca during the tetanus.


A modification of the Digitimer allowing the production of a selected number of pulses separated by a fixed interval.


A comparison of sweat gland activity in eight species of East African bovids. 1. The pattern and control of the sweat glands of eight species of wild bovids has been studied.2. Heat exposure resulted in changes in cutaneous moisture loss which showed considerable species variation. Duiker, Grant's gazelle, Thomson's gazelle and oryx all demonstrated periodic discharges of moisture on to the surface of the skin. Defassa waterbuck and eland exhibited discharges which were accompanied by a gradual rise in basal level. Buffalo showed an immediate large increase which was sustained during the period of heat exposure. Three wildebeest displayed small fluctuations whilst a fourth animal showed a step-wise increment in cutaneous moisture loss.3. The intravenous infusion of adrenaline into the buffalo and one wildebeest caused an increase in cutaneous moisture loss, the magnitude of which was directly related to the size of the dose administered. In three other wildebeest, and all other animals tested, a single injection of adrenaline was necessary to cause a discharge of moisture on to the surface of the skin. Noradrenaline was without effect.4. Heat-induced but not adrenaline-induced sweating was inhibited by the intravenous administration of an adrenergic neurone blocking agent, bethanidine.5. It is concluded that the sweat glands of the wild bovids studied are under adrenergic neurone control, in common with the domestic bovids previously studied, and that there is no correlation between the pattern of sweating and the phylogenetic position of the species.


The response of the sweat glands of the newborn baby to thermal stimuli and to intradermal acetylcholine. 1. Measurements of evaporative sweat loss were made on fifty-six premature and full-term babies 1-67 days after birth with an infra-red analyser and a ventilated capsule placed on the thigh. Measurements were also made of total evaporative water loss while in a closed metabolic chamber and of the regional distribution of sweating with starch-iodine paper.2. No sweating to thermal stimuli could be detected in infants of less than 210 days post-conceptual age, even when rectal temperature rose as high as 37.8 degrees C. In older infants sweat was detected first on the forehead and temple, later on the chest, and usually by 240-260 days post-conceptual age on the legs (term approximately 268 days). Generalized sweating on the limbs appeared at an earlier post-conceptual age in the more prematurely born infants.3. The response of sweat glands on the thigh to an intradermal injection of 2 mug acetylcholine (ACh) was tested. No sweat response was detected in infants under 225 days post-conceptual age, while all infants born within 2 weeks of term responded. The response was often augmented after 2-5 tests at 5-10 min intervals; all the eight infants born within 2 weeks of term who were examined twice in the first 2 weeks of life showed a greater response on the second occasion.4. An average of 414 active sweat glands/cm(2) were detected on the thigh in eight babies 7-10 days old born within 2 weeks of term. This was 6(1/2) times the number found in adults. The mean peak sweat rate to chemical stimulation was however only 2.4 nl./gland.min, which was 3 times lower than the maximum rate recorded in adults.5. In five infants with congenital defects of the brain and complete absence of temperature control there was no sweat response to thermal or direct chemical stimulation of the glands.6. Functional maturation appears to depend on intact central innervation and is marginally hastened by post-natal factors. Immaturity of the sweat glands can account for the lack of any response to thermal stimuli in premature babies, but not for the modest thermal response obtained in babies at term.


Prolonged nembutal anaesthesia and its effect upon the isometric contraction characteristics of some limb twitch muscles of the cat.


A comparison of the tension-time integral of the mechanical activity of the cat fast twitch and slow twitch skeletal muscle in response to repetitive stimulation.


Modulation of noradrenaline incorporation by nerve activities in the rat submaxillary gland. 1. Rats were anaesthetized with chloralose and prepared for electrical stimulation of the cervical sympathetic trunk. The effect of such stimulation was studied on the incorporation of intravenously infused [(3)H]noradrenaline, [(3)H]adrenaline or [(3)H]dopamine by the submaxillary gland.2. A train of 200 stimuli every min for 30 min at 20/sec increased the total incorporation of [(3)H]noradrenaline by 92% over the unstimulated side of the gland, at 50/sec by about 200% and at 3.3/sec by about 30%. The incorporation of [(3)H]adrenaline and of [(3)H]dopamine was also increased by sympathetic stimulation.3. The increases in unaltered [(3)H]noradrenaline and its metabolites were proportional to the increase in total radioactivity.4. Mean venous outflow from the gland was decreased by 15-30% by the sympathetic stimulation.5. After elimination of extraneuronal binding of noradrenaline by ligation of the common excretory ducts, nerve stimulation still increased incorporation.6. Inhibition of normetanephrine production by pyrogallol did not antagonize the nerve impulse-mediated increase of [(3)H]noradrenaline incorporation. alpha-Methyltyrosine was also without effect.7. Desmethylimipramine and bretylium completely abolished the nerve impulse-mediated increase of [(3)H]noradrenaline incorporation in concentrations which did not affect the control uptake or abolish adrenergic transmission.8. Reserpine, guanethidine and phenoxybenzamine antagonized both the control and nerve impulse-mediated incorporations of [(3)H]noradrenaline.9. The results indicate that nerve impulses modulate the incorporation of transmitter amine at the neuronal membrane. Possible mechanisms for this modulation are discussed.


An improved isotonic transducer.

An improved isotonic transducer.


The effect of hypothalamic lesions on the melanocyte-stimulating hormone content and histology of the pars intermedia of the rat pituitary gland. 1. The effect of bilateral lesions in the anterior hypothalamus on the histology and content of melanocyte-stimulating hormone (MSH) of the pars intermedia has been studied in the adult male rat.2. By 7-15 days striking histological changes, suggestive of hyperactivity, were evident. These changes were accompanied by an elevated MSH content, which was particularly noticeable at 7 days.3. By 7 days there was an increase in the acid phosphatase activity of pars intermedia cells, as demonstrated histochemically.4. It was concluded that a hypothalamic control system exists for the mammalian pars intermedia and may involve both inhibitory and excitatory components.


An isometric strain-gauge transducer unit.


Intracellular recordings from ganglia of the thoracic sympathetic chain of the guinea-pig. 1. Intracellular recordings have been obtained from cells of thoracic paravertebral sympathetic ganglia of guinea-pigs.2. Resting potentials were 50-70 mV. The steady-state current-voltage curve was linear for all hyperpolarizations and usually for depolarizations up to 10 mV. The slope of this curve was 19-186 MOmega (mean 55 MOmega). Time constants of cells were 6-14 msec (mean 9.1 msec).3. Most cells maintained a high rate of firing of action potentials during strong current pulses.4. Indirect stimulation via the rami communicantes or the interganglionic nerves of the sympathetic chain evoked a graded synaptic response which could lead to the discharge of one or more action potentials. Hexamethonium blocked the responses to such stimulation. Occasionally an all-or-none response remained, apparently conducted antidromically along the cell's axon.5. Spontaneous synaptic potentials were observed. Their occurrence frequency was increased during indirect stimulation. If they are assumed to represent single quanta of release of transmitter, the quantal content of a threshold synaptic potential was 8-20 quanta.6. Preganglionic fibres could ascend at least three segments or descend two after entering the sympathetic chain. They had conduction velocities of 0.1-1.1 m/sec.7. Inexcitable cells were frequently impaled. Repetitive indirect stimulation gave rise in these cells to a large (20 mV) slow depolarizing response.


Interactions between sarin and the cholinergic antagonist PMCG in fast and slow skeletal muscle of the cat.


Effect of environmental temperature on the turnover of noradrenaline in hypothalamus and other areas of rat brain. 1. The hypothesis that noradrenaline (NA) may be a transmitter in the temperature regulating centre in the hypothalamus is based on the changes in rectal temperature induced by injection of large doses of NA into the brain. As an alternative approach, the effect of environmental temperature on the rate of turnover of endogenous NA in the hypothalamus has been studied.2. Small amounts of tritium labelled noradrenaline [(3)H]NA were injected into the c.s.f. of rats in order to label radioactively the endogenous NA in the brain. The rats were then exposed to environmental temperatures of 9, 17, 24 and 32 degrees C. The rates of disappearance of [(3)H]NA from discrete areas of brain were taken as indices of the rates of turnover of endogenous NA in those areas.3. The rate of disappearance of [(3)H]NA from the hypothalamus was three times as fast at 9 and 32 degrees C as at 17 or 24 degrees C. There were no such significant differences from the pre-optic area or ;rest of brain' (whole brain minus hypothalamus, pre-optic area, cerebellum and medulla).4. The endogenous concentrations of NA were not altered by the experimental procedures in any of the areas of brain studied.5. The rats maintained normal rectal temperatures at environmental temperatures of 9, 17 and 24 degrees C but became 2.8 degrees C hyperthermic at 32 degrees C.6. It is concluded that mild conditions of both heat and cold resulted in an increased turnover of NA in specific nerve terminals in the hypothalamus. Since the rats were thermoregulating normally, the nerve terminals involved are regarded as forming a part of the central temperature regulating centre.


Hormone-induced modifications of free tyrosine in the rat thyroid gland. The concentrations of free tyrosine in liver, muscle, kidney and thyroid gland were determined in separate groups of rats maintained on a low iodine diet and treated with intraperitoneal injections of thyroxine (T 4), thyrotrophin (TSH) and TSH plus propylthiouracil (PTU). Groups of hypophysectomized rats also were given T 4. Significant changes in tissue tyrosine were generally confined to the thyroid gland. Animals treated with T 4 showed a decrease of mean thyroid tyrosine from 113.3 +/- 17.9 (S.D.) mug/g wet weight of gland to 76.2 +/- 5.7 mug/g (P < 0.01). Although there was little change in content when TSH was given alone, a significant increase in tyrosine levels was observed when TSH plus PTU were administered (P < 0.01). In hypophysectomized rats thyroid tyrosine decreased and was further lowered (to 46.0 +/- 3.1 mug/g; P < 0.05) by T 4 treatment. When tyrosine concentrations were expressed in relation to ribonucleic acid (RNA) or protein content of the assayed gland, all differences in tyrosine content became greater.


A two-channel transmitter for the telemetry of cat electrocorticograms.


Some physiological properties of identified mammalian neuroglial cells. Mammalian glial cells were identified and studied in the optic nerves of anaesthetized rats. Cells with membrane potentials of 77-85 mV were located in the optic nerve with capillary micropipettes. These were shown to be neuroglia by iontophoretic injection of a fluorescent dye through the recording electrode, followed by histological verification of the location of the dye. No distinction was made between astroglia and oligodendroglia. Neuroglial cells gave no impulse activity. Their membrane potential was studied in isolated optic nerves by varying the ionic composition of the bathing fluid. The glial membrane potential depends predominantly on a transmembrane gradient of potassium ions.


The spatial selectivity of the visual cells of the cat. 1. Micro-electrode recordings have been made from single units in the visual cortex of the cat, during stimulation by moving grating patterns generated on a cathode ray tube.2. The responses of the units have been measured in terms of either the frequency of impulses, or the contrast sensitivity, and expressed as a function of the spatial frequency of the grating pattern.3. The amplitude of the responses recorded from cortical cell bodies was dependent upon the orientation of the moving grating, and for these units the stimulus was always presented at the preferred orientation. The response amplitude of other units did not depend upon the orientation of the grating stimulus, and these are believed to be the terminations of geniculate fibres.4. The high spatial frequency end of the response function measured in terms of the contrast sensitivity could be well fitted by an exponential function. Subtraction of the blank count from the impulse frequency data gave a curve fitted by the same exponential function. The low frequency end was less consistent.5. The spatial frequency at which this exponential function had fallen by one log. unit was taken to specify the position of the unit's response in the spatial frequency spectrum. For all units these values cover a range of four octaves of spatial frequency (from 0.18 to 3.8 cycles per degree).6. It is suggested that these neurophysiological results support psychophysical evidence for the existence in the visual system of channels, each selectively sensitive to a narrow band of spatial frequencies.


The response of primary afferent fibres from cat spindles to single volleys in sensory nerves.


A counter for magnetic tape recording.

A counter for magnetic tape recording.


On the existence of neurones in the human visual system selectively sensitive to the orientation and size of retinal images. 1. It was found that an occipital evoked potential can be elicited in the human by moving a grating pattern without changing the mean light flux entering the eye. Prolonged viewing of a high contrast grating reduces the amplitude of the potential evoked by a low contrast grating.2. This adaptation to a grating was studied psychophysically by determining the contrast threshold before and after adaptation. There is a temporary fivefold rise in contrast threshold after exposure to a high contrast grating of the same orientation and spatial frequency.3. By determining the rise of threshold over a range of spatial frequency for a number of adapting frequencies it was found that the threshold elevation is limited to a spectrum of frequencies with a bandwidth of just over an octave at half amplitude, centred on the adapting frequency.4. The amplitude of the effect and its bandwidth are very similar for adapting spatial frequencies between 3 c/deg. and 14 c/deg. At higher frequencies the bandwidth is slightly narrower. For lower adapting frequencies the peak of the effect stays at 3 c/deg.5. These and other findings suggest that the human visual system may possess neurones selectively sensitive to spatial frequency and size. The orientational selectivity and the interocular transfer of the adaptation effect implicate the visual cortex as the site of these neurones.6. This neural system may play an essential preliminary role in the recognition of complex images and generalization for magnification.


Excitation of peripheral nerves by direct cooling.


The response of the masseter muscle following tooth contact in man.


The behaviour of cells arrested by contact inhibition.


The provocation of mitosis in the mammalian kidney by folic acid.


Proving a thermostatic microscope stage for rapid adjustment of temperature.


An electrical foot-operated fine-focusing device.


Computer-produced physiological tables for carbon dioxide tension-content interconversions.


A sampling technique for following 32P turnover and net changes in phosphate compounds in crab muscle fibres.


Some effects on the blood vessels of the human forearm of local exposure to pressures below atmospheric. 1. Measurements were made of the initial rate of change of volume of the forearm when the limb was suddenly exposed to various pressures below atmospheric; this was taken to indicate the arterial inflow under these conditions.2. The relationship between arterial inflow and the change in external pressure was curvilinear, with the convexity towards the pressure axis. At -90 mm Hg inflow was increased more than fivefold.3. With short repeated exposures to negative pressure the inflow to the limb showed a progressive decrease for successive exposures until a steady value was obtained.4. The relationship between arterial inflow and the reduction of external pressure during short repeated periods of exposure (6th-10th exposures) was linear from ambient to -80 mm Hg.5. Measurements of forearm blood flow after one, four and ten exposures to reduced external pressure showed that vasoconstriction developed within 20 sec of the end of the exposure and took about 60 sec to disappear. There were only slight differences in the degree of vasoconstriction induced by the different numbers of exposures.


Impulses from epithelial cells of tadpole skin.


Aequorin-monitored calcium transients in single Maia muscle fibres.


The distribution on the surface of the cat cerebellum of some responses evoked via climbing fibre afferents.


Interrelations between the caudate nucleus, mid-brain nuclei, the cerebellum and the lumbar spinal monosynaptic reflexes.


Different muscle fibre types in snake costocutaneous muscle.


Simultaneous recording of vagal and phrenic activity during stimulation of Hering-Breuer reflexes in the rabbit.


Stimulation of the neurohypophysis of the rat by a transaural approach.


The relation between carotid body chemoreceptor activity and carotid sinus pressure in the cat.


Arterial chemoreceptors and the diving response in the duck.


The direct and crossed components of the reflex vasoconstriction in the hind limbs of the dog following stimulation of the carotid body chemoreceptors.


Properties of neurons in long-isolated cortical slabs.


Effects of hyperosmolality on cardiac muscle mechanics in vivo and in vitro.


On the buoyancy of squid of the families Histioteuthidae, Octopoteuthidae and Chiroteuthidae.


Peripheral and striatal responses from the substantia nigra and adjacent reticular formation.


Ventricular fibrillation during hypothermia.


Responses to secretin and pancreozymin in man.


The role of ions in the transport of noradrenaline into synaptosomes.


The absence of effect of gastrin II on skin and voluntary muscle blood flow in a human subject.


A miniature FM transmitter for the radio-telemetry of unit activity.


Isolation of kallikrein-containing granules from the pancreas and submaxillary gland of the cat.


Some vasoactive properties of human plasma.


Ethnic variations in the response of the sweat glands to pilocarpine: the Sudanese.


A hyoscine-resistant excitatory nerve pathway in guinea-pig colon.


Binding sites for amiloride and guanethidine in human blood platelets.


A practical system of foetal electrocardiogram analysis of computer.


The time relation between the secretory and electical responses of supraoptic neurones to osmotic stimuli.


A study of the parameters of electrical stimulation of unmyelinated fibres in the pituitary stalk. 1. The milk-ejection response in lactating rabbits has been used to study the effect of electrical stimuli of different types applied to the supraopticohypophysial tract in the pituitary stalk.2. Sine-wave alternating-current pulses were compared with balanced biphasic square-wave pulses of the same frequency and peak-to-peak current strength. At a pulse duration of 2-4 msec the square-wave stimulation was less effective than the sine wave, but at a pulse duration of 8 msec and over, more effective.3. Above threshold levels of 0.12 mA for the current strength, and of 0.5 msec for the pulse duration, the response increased with increasing current strengths to 2.4 mA and increasing pulse durations to 10 msec.4. With constant and effective strength and duration of the pulse a slight diminution of the response was seen as the frequency was diminished from 100/sec to 50/sec. Further diminution in the frequency revealed that at some point between 50 and 10 c/s a sudden abrupt diminution in the magnitude of the responses occurred over a small range of frequency. This was a reversible phenomenon and it is suggested that it may be related to neurosecretory events occurring in the nerve terminals.5. Stimuli with parameters within the ranges mentioned above are effective in eliciting an oxytocic response on the uterus in the rabbit and ovulation responses (when applied to the hypothalamus) in rats and rabbits. It appears likely that stimuli with such parameters are suitable for experiments concerned with stimulation of the hypothalamus.


Antidromic activation of neurones in the paraventricular nucleus by stimulation in the neural lobe of the pituitary.


Does oxytocin influence the activity of hypothalamic neurones?


Effect of vasopressin on sodium and potassium reabsorption by the renal tubules in man.


Effects of vasopressin on components of Na transport in frog skin.


The ionic specificity of lithium as an inhibitor of the action of vasopressin.


Effects of neurohypophyseal hormones on the skin permeability of Rana cancrivora, in vivo.


Comparison of rebreathing estimates with direct measurements of mixed venous PCO2 and PO2 in man.


The interaction of stimuli to breathing during breath-holding.


The influence of rate of neuronal firing on the time-course of drug responses.


The response to submaximal exercise in adult females; relation to haemoglobin concentration.


A simple double-bridge thermistor thermometer for accurate body temperature measurements.


Comparison of rowing and cycling work capacity tests using heart rate as the parameter.


Phosphate excretion in the laying hen.

Phosphate excretion in the laying hen.


Some effects of protamine zinc insulin on juvenile ducks.


Ultrastructure and histochemistry of granules in the submaxillary gland of the guinea-pig.


Progressive hysterical tetraplegis with contractures. A case study.


The respiratory efficiency and flexibility of erythrocytes stored in acid-citrate-dextrose solution. 1. Measurements have been made of the flexibility and respiratory efficiency of erythrocytes stored in acid-citrate-dextrose (ACD).2. The rate of packing of stored blood, during centrifugation, indicates that the red blood cells are relatively inflexible.3. Spectrophotometric observations, using the rapid-mixing and stopped-flow technique, indicate that the rate of egress of oxygen from HbO(2) in these erythrocytes is significantly reduced. This is not due to a change of the chemical rate of dissociation of HbO(2).4. Neither factor is significantly reversed by resuspending the cells in Ringer-Locke solution or adjusting to pH 7.4.5. A small improvement is obtained by adding hypertonic NaCl or incubating the blood at 37 degrees C for 1 hr.6. Incubation with adenosine, 25 mu-mole/ml. blood, at 37 degrees C for at least 1 hr, restored both the respiratory function and flexibility to normal.


Delayed closure in lung abscess: a re-evaluation of the indications for surgery.


Pulmonary hypertension following Blalock-Taussig anastomosis. Report of a case with pulmonary arterial aneurysm, thrombosis and calcification.


A case of meningitis and generalized Shwartzman reaction caused by Bacillus sphaericus.


Organochlorine insecticide residues in starlings.


Nationwide residues of organochlorine pesticides in wings of mallards and black ducks.


Pesticides in selected Western streams--a progress report.


Residues of DDT and dieldrin in peanuts and tobacco grown on contaminated soil.


Residues of endrin and DDT in turnips grown in soil containing these compounds.


Residues of dieldrin and DDT in peanuts and turnip greens grown in soil containing these compounds.


On heavy metal ions contamination from ampoule glass.


Synthesis of some 4-substituted antipyrine derivatives structurally to local anesthetics.


The use of enzymes in the preparation of vegetable drug extracts.


The pharmacological basis for the rational use of topically applied corticosteroids.


Constituents of local plants. XIV. The antispasmodic principle in Cymbopogon proximus.


Glyceride structure of Egyptian vegetable oils. II. Linoleic acid rich oils.


Investigations on the essential oil of Heracleum mantegassianum L.


Phytochemical investigation of Thesium humile Vahl.


Alkaloids of the leaves of Voacanga schweinfurthii Stapf.


Studies on the fruits of Foeniculum species and their essential oil.


Monosulfates of 16-oxygenated ketonic C19 steroids in adult human urine.


A competitive protein binding method for the measurement of plasma androstenedione.


Radiation life shortening data.

Radiation life shortening data.


The removal of testosterone binding globulin from plasma by affinity chromatography.


Metabolism of progesterone by rattesticular homogenates. 3. Inhibitory effects of intermediates and other steroids.


Metabolism of steroid drugs. I. Different stereospecific hydrogenation of 19-nortestosterone enantiomers by Mycobacterium smegmatis.


Position of the sulfate group in steroid sulfates from human plasma.


Steroid hormone transformations by endocrine organs from pregnant mammals. V. The biosynthesis and metabolism of progesterone and estrogens by orangutan placental tissue in vitro.


Thin-layer chromatographic separation of urinary aldosterone for determination with the tetrazolium blue reaction.


Isodoses of the new wedge filter of the Siemens "Gammatron".


An anti-estradiol effect of ent-estradiol-17beta (1-estradiol).


Excretion of testosterone and epitestosterone glucuronides in pre-school, pre-adolescent and adolescent children.


Metabolism of cortisol in Cushing's syndrome.


Phenoxybenzamine in human shock.

Phenoxybenzamine in human shock.


Speak truthfully to the cancer patient.

Speak truthfully to the cancer patient.


Cone and possible rod components of the fast photovoltage in the frog eye: a new method of measuring cone regeneration rates in vivo.


Light-induced resistance changes in single photoreceptors of Necturus and Gekko.


Neuronal organization of the initial afferent inflow in the cat LGB.


Spectral sensitivity of the parietal eye of the green iguana.


Multiple oil droplets in the photoreceptors of the pigeon.


Progressive supranuclear palsy. Case report with pathological findings.


Basic facilities of application of mathematical modelling in life sciences.


Artifactual elevations of serum alkaline phosphatase following albumin infusions.


Characterization of viruses isolated from bats.


Pulmonary edema of high altitude. II. Clinical, aerohemodynamic, and biochemical studies in a group with history of pulmonary edema of high altitude.


Substrate specificity of collagen proline hydroxylase: hydroxylation of a specific proline residue in bradykinin.


The oxidation of succinate and permeability of dicarboxylate anions in rat-liver slices.


Nonrequirement of exogenous amino acids for protein synthesis in cell-free systems from plants.


Pulmonary ferruginous bodies in city dwellers. A study of their central fiber.


Anomalous aortic origin. Circumflex, anterior descending, or main left coronary arteries.


Chediak-Higashi disease. Morphologic studies of a patient and her family.


Chediak-Higashi syndrome.

Chediak-Higashi syndrome.


Giant cell myocarditis and myositis. Associated with thymoma and myasthenia gravis.