The alpha-chymotryptic ydrolysis of glycine esters. 1. The alpha-chymotrypsin-catalysed hydrolysis of N-acetylglycine ethyl and thiolethyl esters was investigated at pH7.90 and 25 degrees over a wide range of substrate concentrations. 2. The Lineweaver-Burk plots for these substrates are markedly curved, and it is shown that the curvature is due solely to the ;enzyme-blank' reaction. The rate of this reaction is proportional to free enzyme concentration in the range 10-100mum, with a pseudo-first-order rate constant of approx. 1x10(-3)sec.(-1). Correction for this reaction by the procedure described leads to linear plots. It is shown that the significance of the enzyme-blank reaction depends on the value of k(0)/K(m) for the substrate under investigation. 3. Interpretation of the curvature in the Lineweaver-Burk plots by previous workers in terms of activation by excess of substrate is shown to be erroneous. 4. Values of K(m) 387mm and k(0) 0.039sec.(-1), and K(m) 41mm and k(0) 0.23sec.(-1), were obtained for the ethyl and thiolethyl esters of N-acetylglycine respectively. The literature values for the methyl esters of N-acetyl- and N-propionyl-glycine have been corrected by the procedure described. The new values agree much better with current theories of alpha-chymotrypsin mechanism and specificity. 5. The kinetic parameters for the ethyl and thiolethyl esters indicate the absence of an electrophilic component in the catalytic mechanism of alpha-chymotrypsin, and the importance of the ester function in substrate binding.


Purification and properties of adenosine 5'-triphospae-D-glucose 6-phosphotransferase from rat liver. 1. An 870-fold purification of glucokinase from rat liver is described which involves ammonium sulphate fractionation and the use of DEAE-Sephadex, DEAE-cellulose and polyacrylamide columns. 2. The preparation is free of any interfering enzymes and has a specific activity of 8mumoles/min./mg. of protein. 3. Glucokinase catalyses the phosphorylation of glucose, mannose and 2-deoxyglucose. 4. The enzyme is inhibited by high concentrations of glucose 6-phosphate only; ADP is an inhibitor whose effect depends on the Mg(2+) concentration. 5. The properties of glucokinase are compared briefly with those of other phosphotransferases.


Metabolism and biologicalpotency of 5,-monoepoxy-beta-carotene and 5,6:5',6'-diepoxy-beta-carotene. 1. 5,6-Monoepoxy-beta-carotene and 5,6:5',6'-diepoxy-beta-carotene were partially converted into the furanoid forms during passage through the rat stomach. 2. The monoepoxide was converted into vitamin A in the small intestine and showed a biological potency 21% of that of beta-carotene. Neither beta-carotene nor 5,6-monoepoxyvitamin A was formed. 3. Intraperitoneal administration of the monoepoxide led to the accumulation of the unchanged compound in the liver and other tissues. 4. The diepoxide gave no beta-carotene or vitamin A or 5,6-monoepoxyvitamin A when given orally and showed no biological potency. 5. The significance of these results with special reference to the mechanism of formation of vitamin A from beta-carotene is discussed.


The uptake of homologous ribonucleic acid by rat-liver parenchymal cells in suspension. 1. Native or partially degraded RNA derived from intact rat liver, or from the parenchymal-cell or the non-parenchymatous fraction of liver, has been shown to be transported into rat parenchymal cells in suspension, without prior degradation to acid-soluble components, when the cell suspension is incubated with the RNA at 37 degrees . The amount of RNA of exogenous origin present in the parenchymal cells in an acid-precipitable form increased rapidly up to 30-60min., after which it gradually decreased, indicating intracellular degradation to acid-soluble components of the RNA taken up by the cells. 2. The RNA taken up by the parenchymal cells from the medium, and the acid-soluble products of its degradation within the cells, could be released back into the medium. 3. The RNA of exogenous origin present in acid-precipitable form in the parenchymal cells represented up to 5% of the RNA of the cells after 60min. of incubation. 4. When the concentration of RNA in the medium was less than 200mug./ml., over 10% of the RNA was transported in an acid-precipitable form in 60min. into the parenchymal cells incubated at a concentration of 2.3x10(6)/ml. 5. Ribonuclease inhibited the uptake of exogenous RNA by the parenchymal cells, whereas 2,4-dinitrophenol, sodium azide, protamine sulphate and polyvinyl sulphate had no significant effect. 6. The uptake of exogenous RNA by liver slices proceeded at a rate which was 4-20% of that obtained in the parenchymal-cell suspensions; the RNA taken up did not appear to become degraded, unlike that taken up by the cell suspensions. 7. It is concluded that dispersion of liver tissue to a suspension of single cells increases the permeability of the parenchymal cells to macromolecular RNA and creates conditions that lead to a rapid degradation of the RNA taken up.


Studies on lipogenesis in vivo. Effect of dietary fat or starvation on conversion of [14]glucose into fat ad turnover of newly synthsized fat. 1. Lipogenesis was studied in vivo by giving mice 250mg. meals of [U-(14)C]glucose and measuring the disposition and incorporation of label. About 48% of the (14)C dose was eliminated as (14)CO(2) in the first 2hr. At 60min. after administration, 1.0, 1.9 and 11.9% of the dose was recovered as liver glycogen, liver fatty acid and carcass fatty acid respectively. Of the [(14)C]glucose converted into fat in the epididymal pads about 90% was present as glyceride fatty acid and 10% as glyceride glycerol. 2. Hepatic synthesis of fatty acid was depressed by dietary fat to a much greater extent than was synthesis outside the liver. Both feeding with fat and starvation decreased the proportion of the label taken up by adipose tissue present as fat (triglyceride) and increased the proportion of triglyceride label present as glyceride glycerol. These results are consistent with the hypothesis that the primary action of both these conditions in decreasing fat synthesis is to inhibit synthesis of fatty acids. 3. Turnover of body fat labelled in vivo from [U-(14)C]glucose was estimated from the decline in radioactivity measured over the first 24hr. of the experiment. The half-life of liver and extrahepatic fatty acids (excluding epididymal fat) was 16hr. and 3 days respectively. In contrast, no measurable decrease in radioactivity of the fatty acids of epididymal fat was observed for 7 days after administration of the [U-(14)C]glucose.


nzymic conversion of 3-hydroxyanthranilic acid into cinnabarinic acid. Partial purification and properties of ra-liver cinnabarinate synthase. Rat-liver cinnabarinate synthase (3-hydroxyanthranilic acid-oxygen oxido-reductase) was partially purified. Stoicheiometric studies indicated the consumption of 3 atoms of oxygen/molecule of cinnabarinic acid formed. There was an initial lag in enzyme activity. The reaction had an optimum pH about 7.2 and an optimum temperature of 37 degrees . The enzyme was highly specific for 3-hydroxyanthranilic acid. The system showed an absolute requirement for Mn(2+) ions. Several bivalent metal ions and metal-chelating agents inhibited the reaction. Thiol inhibitors had no effect on enzyme activity, but reducing agents such as ascorbic acid were potent inhibitors. There was no requirement for any cofactor other than Mn(2+) ions. The probable significance of the reaction in mammals is discussed.


Stdies on lipogenesis in vivo. Effects of starvation andre-feeding, and studies on cholesterol synthesis. 1. Studies in vivo have been carried out on hepatic and extrahepatic cholesterol synthesis and also on the effects of starvation and re-feeding on both cholesterol and fatty acid synthesis. 2. In rats and mice fed on a stock diet, extrahepatic tissues accounted for about 4 times as much newly synthesized cholesterol as did the liver. The liver appeared to be somewhat more important in the rat than the mouse. Feeding with cholesterol greatly decreased and cholestyramine greatly increased hepatic cholesterol synthesis without much effect on extrahepatic synthesis. 3. Mice starved for up to 7hr. did not lose any of the ability to convert a [U-(14)C]glucose meal into fat, whereas 18hr. of starvation resulted in an 80% loss of fatty acid synthesis in liver and carcass, an 80% loss in liver cholesterol synthesis and a 65% decrease in carcass cholesterol synthesis; 18hr. of food deprivation also decreased the proportion of counts in epididymal fat pads present as fat and increased the proportion present as glyceride glycerol. 4. Re-feeding for up to 7hr. restored fatty acid synthesis from a [U-(14)C]glucose meal to about 50% of the values for non-starved mice but had no effect on hepatic cholesterol synthesis. The altered distribution of counts in the epididymal fat pads caused by starvation was restored to normal after feeding for 1hr.


Effects of insulin-induced hypoglycaemia on the fate of glucose carbon atoms in the mouse. 1. [U-(14)C]Glucose was injected into mice and the distribution of (14)C in various chemical fractions of the whole body was determined at times from 15min. to 8hr. after injection. 2. At 1hr. after injection 31.8% of the recovered (14)C was found in the expired air and 26.7% was found in the isolated glycogen, lipids, proteins, nucleic acids and in other acid-insoluble carbon compounds (;residual (14)C'). The rest (41.5%) was combined in acid-soluble substances. 3. When insulin was injected 5min. or 1hr. before injection of [U-(14)C]glucose, and the mouse was killed 1hr. later, the (14)C content of expired air, glycogen, protein and ;residual (14)C' was not significantly affected; but the incorporation of (14)C into lipids was increased two- to three-fold. 4. Chromatography of the lipids on silicic acid columns and by thin-layer chromatography showed that the main effect of insulin injection was to increase the incorporation of (14)C into fatty acids. 5. A significant increase of (14)C after insulin injection was also found in a glyceride in which the (14)C was combined in glycerol.


Nature of Cu(II)-poly(glutamic acid) complex in aqueous solution.


Interactions of calf-thymus histone fractions in aqueous solution with 8-anilinonaphthalene-1-sulphonic acid. 1. The interactions of histone fractions with 8-anilinonaphthalene-1-sulphonic acid were investigated by fluorimetry and spectrofluorimetry and the results were interpreted with the aid of equilibrium-dialysis techniques. 2. Characteristic differences were found between the various histone fractions, and with fractions F3 and F2a the binding was found to be salt-dependent. 3. Evidence was obtained indicating a slow change of the physical state of fractions F3 and F2a in the presence of salt, and the binding by these two fractions in the presence of salt was greater by an order of magnitude than by fractions F1 and F2b. 4. Conditions favouring binding were also those favouring histone aggregation; SO(4) (2-) ions activated binding at a lower concentration than Cl(-) ions; urea, guanidinium ions and high concentrations of I(-) ions were inhibitory to binding. 5. After histones had been kept in the presence of salt for a long time the reversal of interaction on decreasing the salt concentration was incomplete. 6. The inhibition of binding by fraction F2a in the presence of urea or fraction F2b depended on the time sequence of addition of the reagents. 7. Artificial nucleoproteins made by precipitating DNA with the histone fractions in neutral 0.14m-sodium chloride showed the same order of interaction as was found for the fractions in solution. 8. Comparison of the binding by fraction F2a with that by bovine plasma albumin showed that in both cases there were a large number of weakly binding sites but that fraction F2a lacked the small number of strongly binding sites found in albumin. No slow change of binding in the presence of salt was found for albumin. 9. Binding by fraction F2b increased the affinity of the protein for further molecules of the adsorbate. 10. The results are discussed in relation to the close relationship between binding and aggregation and the possible role of non-polar interactions as determined by the balance between polar and non-polar amino acids in the histone fractions.


Sedimentation coefficient of T-even bacteriophage DNA.


The interaction of eucocidin with the cell membrane of the polymorponuclearleucocyte. 1. When leucocidin is incubated with leucocytes it is inactivated in solution and only a little adsorption takes place. This reaction has been used to purify the cell membrane. 2. The interaction of the membrane with leucocidin is very complex and at least three phenomena occur: (a) An inactivation of leucocidin in solution by large amounts of membrane which is synergistic between the two components of leucocidin, is thermolabile and is not inhibited by electrolyte. (b) An adsorption of leucocidin which is synergistic between the two components of leucocidin, does not proceed to the same extent as the inactivation in solution and is a function of the phospholipid components. Phospholipids isolated from the membrane adsorb leucocidin but the adsorption requires the presence of several molecular species. (c) Polymerization of leucocidin induced by tenfold smaller amounts of membrane than are required to bring about the first two interactions. The polymerization is reversed by adjustment of the ionic strength. It is due to the presence of the lipid components of the membrane. Different lipids are equally effective in inducing the polymerization. 3. Each component of leucocidin will polymerize in the absence of membranes and lose biological activity at low ionic strength. This is reversed by electrolyte and it does not proceed to the same extent as in the presence of membranes. 4. The nature of the interaction of leucocidin with cells, membranes and lipids and the spontaneous polymerization indicate that each component of leucocidin can adopt different isomeric forms. 5. The relationship of the interaction with the membrane to the cytotoxic effect of leucocidin is discussed.


Base composition of intact nucleic acid oligomers.


Solution properties of synthetic polypeptides. I. Light scattering and osmometry of poly-gamma-benzyl-L-glutamate in helicogenic solvents.


Topological considerations in protein structure. I. Disulfide linkage.


The formation of bilirubin and p-nitrophenyl glucuronides by rabbit liver. 1. Glucuronide formation of bilirubin and p-nitrophenol in vitro with excess of UDP-glucuronic acid by UDP-glucuronyltransferase from livers of young and adult rabbits was studied. 2. The development of UDP-glucuronyltransferase for the two substrates followed a markedly different pattern during maturation of young rabbits, p-nitrophenol-conjugation ability being much higher at birth than that for bilirubin. 3. Mg(2+) increased bilirubin conjugation, but inhibited p-nitrophenyl glucuronide formation. 4. p-Nitrophenol acted as a potent non-competitive inhibitor for bilirubin conjugation but bilirubin did not affect p-nitrophenyl glucuronidation. 5. The enzyme for bilirubin conjugation was inactivated at pH9 during treatment with snake venom, whereas in the same preparation the activity of the corresponding enzyme for p-nitrophenol was enhanced. In addition, some solubilization of the latter enzyme could be achieved by this method. 6. The possibility of the existence of more than one enzyme system for the formation of O-glucuronides is discussed.


Reconstruction of protein and nucleic acid sequences. V. Computer-simulated tests of various tactics for reconstructing the sequences of transfer ribonucleic acids.


Electro-optical studies of conformation and interaction of polynucleotides.


Solution properties of synthetic polypeptides. II. Sedimentation and viscosity of poly-gamma-benzyl-L-glutamate in dimethylformamide.


Multiple myeloma and normal pregnancy. Report of a case.


Studies on glycopeptides deried from acidic glycoproteins of guinea-pig serum. 1. Fraction I, a fraction containing acidic glycoproteins, isolated from guinea-pig serum, was digested with Pronase after removal of sialic acid and a major and a minor glycopeptide fraction were isolated by chromatography with Sephadex G-25 and G-50. 2. The major fraction was examined by various methods and shown to contain several glycopeptides. Estimates of molecular weight of the glycopeptide fractions were obtained. Although some variation appeared to occur, the glycopeptides were not grossly heterogeneous with respect to size. An average prosthetic group was estimated to contain about 15 sugar residues. 3. Aspartic acid was the principal amino acid present in the fractions and in all subfractions of the major fraction investigated. Where examined, ammonia was liberated on acid hydrolysis in approximately equimolar amounts to the aspartic acid present. The carbohydrate composition of the fractions was also determined. 4. The glycopeptides showed relatively little degradation in alkaline solution. 5. These results suggest that an N-acylglycosylamine bond involving aspartic acid forms the major type of linkage between carbohydrate and polypeptide. The isolation of a compound with the composition and chromatographic properties of 2-acetamido-1-(l-beta-aspartamido)-1,2-dideoxy-beta-d-glucose supports this view, and indicates that N-acetylglucosamine is the sugar involved in at least many linkages. 6. Fraction I contains some glycoproteins that are susceptible to Pronase and one or more others that resist digestion before the removal of sialic acid. A brief examination revealed some similarities between prosthetic groups derived from both kinds of glycoprotein.


Studies in the pathogenesis of autoerythrocyte sensitization syndrome.


Quantitative estimation of split products of fibrinogen in human serum, relation to diagnosis and treatment.


Factor 13 deficiency with severe hemorrhagic diathesis.


Effect of thymus enclosed in millipore diffusion envelopes on thymectomized hamsters.


Absence of cis delta chain synthesis in (sigma beta) thalassemia (F-thalassemia).


The iron content of jejunal mucosa obtained by Crosby's biopsy in hemochromatosis and hemosiderosis.


Plasma cells containing iron. An electron micrographic study.


Fe59 absorption in human subjects using total-body counting technic.


Observations on certain enzyme activites of normal human red cells treated with sulfhydryl compounds.


Cancer in Sarawak (Borneo). A preliminary survey.


Nasopharyngeal cancer in the Malays.

Nasopharyngeal cancer in the Malays.


A case of multiple malignant tumours successfully treated by surgery.


Enteropathy in malignant disease.

Enteropathy in malignant disease.


A trial of vinblastine sulphate in the treatment of inoperable carcinoma of the lung.


Uptake of [1,2-3H]17-alpha-methyltestosterone by breast carcinoma and other tissues of human subjects.


The effect of therapeutic radiation on serum and urine beta-glucuronidase.


The results of prolonged administration of isoniazid to mice, rats and hamsters.


Biochemical studies on human and rat breast tissues.


Inhibitory effect of injection of Corynebacterium parvum on the growth of tumour transplants in isogenic hosts.


Further observations on the growth of mouse mammary carcinomata in the strain of origin. Attempts to transfer adoptive immunity against the tumour, to isogenic hosts, with spleen cells from tumour-bearing mice.


Incidence of lung tumours induced by urethane in mice exposed to reduced atmospheric pressure.


Carcinogenesis in naturally tumour-resistant mice. X-irradiation versus urethane as a carcinogenic agent.


Further observations concerning the influence of preliminary stimulation by croton oil and acetic acid on the initiation of skin tumours in mice by urethane.


Parasites of the Canada lynx, felis (lynx) Canadensis (Kerr).


Methylcholanthrene carcinogens in the Swiss albino mouse in relation to differential oncogenesis of skin tumours.


The effects of propiolactone on rat fibrocytes cultivated in vitro.


The effect of age on temperature selection by laboratory mice (Mus musculus).


The transmission of Pneumostrongylus tenuis to guinea pigs.


Some sources of variation in the blood sera of deer (Odocoileus) as revealed by starch gel electrophoresis.


Chromosome studies on 50 species of aphids.


A study of the inhibitory effects of chick whole eye extract on the development of the lens of the chick embryo in vitro.


The salivary gland chromosomes of six species in the 3S-1 group of Prosimulium roub. (Diptera: Simuliidae).


The stomodaeal nervous system, the neurosecretory system, and the gland complex in Aedes aegypti (L.) (Diptera: culicidae).


Early days of hospital dietetics.

Early days of hospital dietetics.


Proximate composition of ready-to-serve potato products.


Color of cooked carrots related to carotene content.


The dietetic intern is initiated into the medical team.


The behavioral sciences and technologies--impact on management.


Induction of heme-protein enzyme in animal cells. IV. Relationship between rapidly labelled RNA and induced-synthesis of tryptophan oxygenase in vivo.


Studies on mercapturic acids. Effect of some aromatic compounds on the level of glutathione and the activity of glutathionase in the rat.


Some aspects of D-glucuronolactone dehydrogenation by guinea pig liver enzyme [EC 1.1.1.70].


Fractionation of peptides obtained by chymotryptic hydrolysis of Baker's yeast cytochrome c.


Distribution of fibroin in subcellular fractions of posterior silkgland of the silkworm, Bombyx mori L.


Effect of secondary structure of DNA upon solubility of aromatic hydrocarbons.


Studies on nonspecific adenosine deaminase from Takadiastase. II. Studies on the structure of the substrates.


Calorimetric studies on hydrolysis of glucosides. IV. Calorimetric determination of alpha-1,4 glucosidic linkage content in some starches and glycogens.


Incorporation of amino acids by cell-free systems from regenerating rat liver.


Studies on the biosynthesis of glycine in the silkworm. 3. Formation of glycine from C14-glycolic acid and the occurrence of glycolate oxidase.


Migration of injected C14-labelled riboflavin into rat tissues.


Rapidly reducible and slowly reducible complexes of heme a.


Crystal-induced inflammation in canine joints. II. Importance of polymorphonuclear leukocytes. In an experimental model, the polymorphonuclear leukocyte was found to be necessary to the inflammation induced by crystals. This conclusion is based on (a) the invariable migration of polymorphonuclear leukocytes into the synovial fluid of canine joints injected with urate crystals, (b) the experimental suppression of the synovitis by the depletion of polymorphonuclear leukocytes in animals treated with vinblastine, (c) the dependence of the degree of suppression on the degree of leukocyte mobilization into the joint space, and (d) the restoration of the inflammatory response in a leukopenic animal by perfusion with normal blood.


An antiviral substance from Penicillium funiculosum. VI. Prevention of the establishment of passive immunity to Semliki Forest virus infection in mice by Helenine. 1. Helenine prevents the establishment in mice of passive viral immunity by anti-Semliki serum of swine, rabbit, or guinea pig origin. 2. A period of 12 days must elapse, between the antiviral serum administration and challenge with virus, for prevention of the establishment of passive immunity to become apparent. This period is believed to correspond to that in which injected antibody persists in circulation in the injected host. 3. Helenine is effective in preventing the establishment of passive viral immunity by heterologous antiviral sera when it is administered any time during a period of 6 days, extending from 4 days before to 2 days after injection of the antiviral serum. 4. Helenine does not prevent the establishment of passive viral immunity by antiviral sera of mouse origin (homologous). 5. Evidence is presented to indicate that the phenomenon of the prevention of the establishment of passive viral immunity by heterologous antiviral sera is not effected directly, but rather is mediated through some substance that helenine induces the injected host to elaborate. 6. The capacity to prevent the establishment of passive viral immunity could not be exhausted by repeated preceding injections of helenine at 2- or 3-day intervals. 7. Evidence is presented to indicate that the helenine-induced material does not act upon antiviral antibody per se but rather on heterologous foreign protein that happens to be labeled as Semliki Forest virus antibody. This helenine-induced material, whatever its nature, appears to enhance the capacity of the injected host to recognize and dispose of foreign protein. 8. Statolon, a material that like helenine is a known inducer of interferon, is, like helenine, also capable of preventing the establishment of passive viral immunity by heterologous antiviral sera. 9. Experiments designed to determine whether the induced material responsible for the antipassive immunity effect of helenine is interferon have yielded inconclusive answers thus far.


Quantitative studies of the adoptive immunological memory in mice. I. An age-dependent barrier to syngeneic transplantation. Antibody-forming cells suspended from a mouse spleen and transferred to intact animals of the same genotype face a barrier which severely affects their capacity to implant and/or to function. This phenomenon was quantitatively studied in a model system which, utilizing the immunogenic properties of human serum albumin in mice, allows the secondary response of the transferred cells to be followed without interference from the host's own reactivity. The barrier to syngeneic transplantation was found (a) to be radiosensitive (500 R X-rays to the recipient abolishes it and insures optimal functional conditions to the donor cells) in the same order of magnitude of other mammalian systems involving rapidly dividing cell populations, and (b) to depend upon the age of the recipient: its linear rise is documented from birth time (when approximately 50% of the maximal immune capacity of the transfer is expressed) to the age of 2 months ( approximately 1 %). The significance of these findings to the immune response and to cell growth and differentiation is discussed.


Ontogeny of the immune response. I. The development of the follicular antigen-trapping mechanism. Polymerized flagellin from Salmonella adelaide was labeled with I(125) and injected into rats varying in age from 0 to 42 days. Lymphoid organs were removed at various intervals and the progressive development of antigen-capturing structures was studied using autoradiography. The chief findings were as follows: 1. Newborn rats lack the follicular and medullary antigen-trapping structures characteristic of adult animals. 2. At the age of 10 to 14 days, the first signs of specific cortical antigen localization appear in lymph nodes. This initially takes the form of a continuous "cortical rim" of antigen localization. 3. Within a further 4 to 6 days, the Anlagen of true follicular antigen-capturing structures appear, the continuous rim being only a transitional mechanism. 4. The antigen-capturing part of the follicle appears before the lymphoid component; follicle Anlagen can be defined only on autoradiographs and cannot be seen on ordinary histological sections. 5. The system of medullary macrophages develops gradually over the period 2 to 6 weeks of age. 6. The ability of lymph nodes to retain antigen increases progressively, there being a fivefold increase in the amount of antigen retained per unit weight of lymphoid tissue between 2 and 6 wk of age.


Concomitant anaphylactic sensitization and contact unresponsiveness following the infusion or feeding of picryl chloride to guinea pigs. Guinea pigs receiving one large dose of picryl chloride by the intravenous or oral routes commonly develop circulating antibody demonstrable by passive cutaneous anaphylaxis or by active anaphylaxis. They often concommittantly become unresponsive to the induction of delayed contact hypersensitivity by intracutaneous injections. Erythrocytes obtained from guinea pigs after infusion or feeding of picryl chloride may be used to sensitize other animals when injected with adjuvant. It is concluded that guinea pigs may be anaphylactically sensitized to simple chemicals by the intravenous and oral routes if a sufficient dose is administered.


Ontogeny of the immune response. II. Correlations between the development of the afferent and efferent limbs. The development of the ability of young rats to generate a prompt primary antibody response to polymerized flagellin, with IgM to IgG transition, is correlated in time with the development of structures in the cortex of lymph nodes that localize antigen to spherical areas which subsequently become primary lymphoid follicles. Throughout development the increased magnitude of the antibody response parallels the increased ability of lymphoid structures to retain antigen. During the first week of life primitive lymphoid tissue appears capable of undergoing the initial steps in differentiation toward antibody production in response to neonatal injections of polymerized flagellin. However, further maturation appears to be blocked resulting in a complex immunological state at the age of 2 weeks characterized by increased IgM and decreased IgG antibody response to antigenic challenge at this time. The possible relationship between the block in cellular differentiation toward antibody formation and the ease of tolerance induction is discussed.


Hypertrophy of the agranular endoplasmic reticulum in hamster liver induced by phenobarbital (with a review on the functions of this organelle in liver).


Infectious virus-antibody complex in the blood of chronically infected mice. If viremic sera from mice chronically infected with lactic dehydrogenase virus (LDV) were first treated with ether or ultraviolet light to inactivate the infectious virus, neutralizing antibody could be demonstrated. Significant amounts of antibody, however, were not detected until the mice had been infected for about 2(1/2) months and its presence did not result in the elimination of the chronic viremia. Virus isolated from sera containing neutralizing antibody was found to be relatively resistant to neutralization by anti-LDV. Further studies revealed that the resistant virus existed in the form of an infectious virus-antibody complex (sensitized virus). The presence of such a complex was demonstrated by the fact that the virus fraction which persisted after in vivo or in vitro exposure to mouse anti-LDV was readily neutralized by goat anti-mouse sera or goat anti-mouse gamma-globulin, whereas virus that had not been previously exposed to mouse anti-LDV was completely resistant to neutralization by goat anti-mouse sera. These findings suggest that (a) sensitization may play an important role in the resistance and susceptibility of a virus to neutralization by antiviral antibody, and (b) an anti-gamma-globulin may prove useful in neutralizing the resistant fraction and in demonstrating otherwise undetectable antiviral antibody.


The simultaneous estimation of uric acid and urea with the autoanalyser.


Enzymorphologic demonstration of glucose 6-phosphate--dependent glycogen synthetase in mouse liver.


Tests of platelet function and their use in the investigation of bleeding disorders.


A method for the preparation of urine deposits for cytological examination.


Blood urea estimation.

Blood urea estimation.


Alloxan-schiff reagent for the detection of fixed rabies virus in the nerve cell.


A Klebsiella screening medium.

A Klebsiella screening medium.


Logistical problems of toxicological pathology.


A rapid method for the detection of plasma phenylalanine.


A section counter for the Leitz rotary microtome, model 1212.


Observations on the role of calcium ions in ganglionic responses to acetylcholine.


Demonstration of catecholamines and 5-hydroxytryptamine in the human carotid body.


Relationship between smooth muscle contraction and phosphorylase activation.


Effects of cesium, potassium and rubidium on development of tension in isolated human uterine muscle.


Postganglionic sympathetic delay in vascular smooth muscle.


Hypotensive activity of beta-heptamethylenimino-ethylaminoguanidine sulfate.


The cardiovascular pharmacology of lidoflazine, a long-acting coronary vasodilator.


The effect of disulfiram on the restoration of the response to tyramine by dopamine and alpha-methyl-dopa in the reserpine-treated cat.


Subcellular localization of H3-norepinephrine in the rat brain and the effect of drugs.


A comparison of the effects of neuromuscular blocking agents and cholinesterase inhibitors on the tibialis anterior and superior rectus muscles of the cat.


Participation of a CNS-thyroid neural pathway in epinephrine-induced hyperglycemia in the cat.


Acute tolerance indices for pentobarbital in male and female rats.


Differential effects of two phenothiazines on chain and tandem schedule performance.


Chemical and biochemical studies on vitamin B12 and its related compounds. XVI. Factors affecting the activity of a dioldehydrase requiring cobamide coenzyme. Enhancement by anaerobic light-irradiation and competitive inhibition by adenosine.


Studies on thiamine monophosphate disulfide. I. Synthesis of thiamine monophosphate disulfide.


Studies on myoinositol. II. Possible role of dietary myoinositol on the growth promotion of young rats.


Physiochemical properties and quantitative determination of thiamine monophosphate disulfide.


Biological and pharmacological properties of thiamine monophosphate disulfide.


On the metabolites of ascorbic acid, especially oxalic acid, eliminated in urine, following the administration of large amounts of ascorbic acid.


Interaction of thiol-type thiamines with serum albumin.


Effect of ethyl linoleate, ethyl linolenate and ethyl esters of highly unsaturated fatty acids on essential fatty acid deficiency in rainbow trout.


Formation of deoxyinosine from inosine by washid cell suspension of vitamin B12-requiring strain of Lactobacillus leichmannii.


Venous by-pass grafts for arterial obstruction in the lower limbs.


The hospital's role in the community.

The hospital's role in the community.


Migrainous neuralgia.

Migrainous neuralgia.


Skin cancer and hyperkeratoses due to sunlight.


Auscultation and phonocardiography in acute myocardial infarction.


Loss of entire mid-gut with survival.

Loss of entire mid-gut with survival.


The neurological complications of pertussis.


Vascular surgery.

Vascular surgery.


Effect of hydrogen ion concentration on the in-vitro viscosity of packed red cells and blood at high haematocritis.


Liability of the military doctor.

Liability of the military doctor.


A prognostic study of juvenile rheumatoid arthritis. Analysis of 544 cases.


Vasopressin action on the cephalic vasculature. Angiographic study in the dog.


Percutaneous balloon-occlusion of the aorta.


Extracardiac anastomoses to the myocardium. Preliminary report of angiocardiographic and anatomic studies.


Vertebral genesis of idiopathic scoliosis in children.


Experiences with gamma-encephalography. II.


Collaterals in obstruction of the renal artery.


Determination of optimum film density range for roentgenograms from visual effects.


Bivalent alleles.

Bivalent alleles.


On the familial predisposition to favism.


Transmission of a D/D reciprocal translocation in a family with high incidence of mental retardation.


Anti-M, a new antibody of the MNS blood group system. I. Mv, a new inherited variant of the M gene.


Factors influencing cholinesterase activity in a Brazilian population.


Estimation of nonpaternity.

Estimation of nonpaternity.


Pyogenic liver abscess studied by cholangiography. Case report and 25-year review.


Microsurgery in silastic vascular grafts and arteriovenous cannulas. Testing with teflon tips, dacron skirt and stainless steel strands.


Hypothermia as a surgical adjunct. Review of 40 cases.


The distribution of glucose-6-phosphate dehydrogenase deficiency in Greece.


Gigantic solitary congenital cyst of the lung in a nine-year-old boy: case report.


Superior mesenteric arterial occlusion and intestinal infarction. Clinical evaluation.


Arteriovenous fistula in pulsatile metastatic carcinoma. Report of chronic heart failure relieved by resection.


Primary carcinoma of extrahepatic bile ducts. Review of 22 cases.


Leiomyosarcoma of the stomach. Report of nine cases.


Primary nonspecific ulcer of the ileum following lateral intestinal anastomosis. Report of two cases.


Pseudocyst of the pancreas. Report of three cases.


Whither duodenal ulcer surgery?

Whither duodenal ulcer surgery?


Ossifying fibroma of the long bones. A light and electron microscopic study.


Congenital tumors of the parotid gland.

Congenital tumors of the parotid gland.


Allergic encephalomyelitis in Chediak-Higashi mink. Encephalomyelitis, ganglionitis, and neuritis.


Lipid nature of ceroid in experimental nutritional cirrhosis.


Wound healing in lysine deficiency.

Wound healing in lysine deficiency.


Spontaneous arterial lesions in the antarctic seal.


Electron microscopic observations on skeletal muscle in diffuse scleroderma.


Tissue antibodies in chronic lupus erythematosus.


The radiological manifestations of scleroderma (Diffuse systemic sclerosis).


Electron microscopy of the renal lesions in systemic lupus erythematosus.


The cutaneous lesions of systemic and chronic discoid lupus erythematosus and porphyria symptomatica in the same patient.


Vascular changes in the cutaneous lesions of lupus erythematosus and scleroderma.


Studies of the leucocyte compartment in guinea-pig bone marrow after acute haemorrhage and severe hypoxia: evidence for a common stem-cell.


The effect of unilateral limb shielding on the haemopoietic response of the guinea-pig to gamma irradiation (150 r.).


The cytogenetics and cell metabolism of circulating Reed-Sternberg cells.


Sideruria following a single dose of desferrioxamine-B as a diagnostic test in iron overload.


A procedure for separating viable lymphocytes from human blood and some studies on their susceptibility to hypotonic shocks.


The amino acid content of venous blood and marrow aspirate before and after treatment in pernicious anaemia.


The response of guinea-pig bone marrow to intravenous injection of TAB vaccine.


Analysis of erythroid homeostatic mechanisms in normal and genetically anaemic mice.


The role of lymphography in the diagnosis and management of malignant reticuloses.


Observer error in the radiological diagnosis of Paterson-Kelly webs.


Vascular lesions of the colon.

Vascular lesions of the colon.


Radiological findings in globus hystericus.


Cineradiology with image intensification: an analysis of the effects of camera and projector shutter design and related technical aspects.


A radiological method for the study of small bony specimens (rodent mandibular radiology).


The effect of single doses of x-radiation on mandibular growth.


The metabolism of scandium 47 produced by the decay of calcium 47 in vivo.


Radiation cataract--new evidence on radiation dosage to the lens.


The use of x-rays to produce transmission pictures of virus models.


Linear accelerator simulator.

Linear accelerator simulator.


Modification of a Baldwin radiological densitometer to measure densities up to 7.5.


Non-traumatic pulmonary haematoma.

Non-traumatic pulmonary haematoma.


Physico-chemical requirements for beta-sympatholytic action.


Propylthiouracil and transfusion modifications of the effects of the Rauscher virus in BALB/c mice.


Development of thymic neoplasms in rats inoculated with a murine leukemia virus (Rauscher).


The C3H/St/Ha mammary tumor. I. Thromboplastin content.


Chronology and pattern of human chromosome replication. 3. Autoradiographic studies on cells from cancer effusions.


Adaptation, growth, and chromosomal analysis of HEp-2 cells in chemically defined medium.


Problems raised by the search for virus particles in human leukemia. A study with the electron microscope of blood plasma, cerebrospinal fluid, and megakaryocytes from bone marrow.


Characterization of the H-2 antigens of L-M mouse cells grown in culture.


Antitumor immunity in the Shope papilloma-carcinoma complex of rabbits. 3. Response to reinfection with viral nucleic acid.


Mammary tumors and mammary gland development in normal and estrogen-treated F-1 hybrids of strains C3H/J and R3/An mice.


Morphology of BALB/c mice inoculated with Rauscher virus.


Preparation, purification and structure of endotoxin.


The present status of the problem of endotoxin shock.


Norepinephrine, dibenzyline and endotoxin shock.


Endotoxin shock in man and in the dog.

Endotoxin shock in man and in the dog.


Endotoxin shock as a syndrome of disseminated intravascular coagulation.


Hyperbaric oxygen in shock.

Hyperbaric oxygen in shock.


Evaluation of therapeutic agents: morphologic changes and survival data in dogs subjected to endotoxin during the shock tour.


Information theory and figure perception: the metaphor that failed.


The nature of the heart dipole.

The nature of the heart dipole.


Empiric risk data for certain common anomalies and comments regarding tiological factors.


Diagnostic methods in biliary tract disease.


A practical guide to the use of diuretics.


Prestige for th teacher.

Prestige for th teacher.


Modern auscultation and ponocardiography--a review with loudspeaker presentation of heart sounds.


Neurologic anagrams.

Neurologic anagrams.


Pituitary changes in head trauma (analysis of 102 consecutive cases of head injury).


An index of malocclusion for use in multiphasic screening and epidemiological investigations.


Medical College f Alabama--student affairs; medical student--1965-66.


The Birmingham, labama, 1964 diabetes detection drive. I. Age and dextrostix patterns.


Utilization of the non-pofessionally trained social worker in a health setting.


Alabama needs more physicians.

Alabama needs more physicians.


Miniature ECG amplifiers.

Miniature ECG amplifiers.


A molecular basis for the mechanism of insulin action.


Vascularization of the parietal pleura. Experimental study.


The effect of hexokinase and tricarboxylic acid-cycle intermediates on fatty acid oxidation and formation of ketone bodies by rat-liver mitochondria. 1. The oxidation of butyrate, hexanoate and octanoate by rat-liver mitochondria suspended in a tris-potassium chloride medium in the presence of malate and serum albumin has been investigated. 2. The oxidation of butyrate to acetoacetate was markedly decreased by the addition of a system competitive for ATP (hexokinase-glucose). 3. Serum albumin or tricarboxylic acid-cycle intermediates prevented the inhibition by hexokinase and in their presence a greater proportion of the oxygen consumption was contributed by the tricarboxylic acid cycle. The results suggest that the energy supply for fatty acid activation is either compartmentalized in a spatial or kinetic sense or there exists a special activating mechanism not involving ATP. 4. Malate and other tricarboxylic acid-cycle intermediates caused substantial reduction (to beta-hydroxybutyrate) of the acetoacetate formed during the oxidation of butyrate, hexanoate and octanoate.


[The contribution of pathological anatomy in the procedure to follow in tumors of the bladder].


Studies on synthesis of epoxides of anhydrocardenolides and on their cleavage. II. Synthesis of epoxides of anhydrogitoxigenins and their cleavage.


Use of the activated partial thromboplastin time in the control of heparin administration.


Initial neurological symptoms in systemic lupus erythematosus.


Collaboration: a negotiated contract.

Collaboration: a negotiated contract.


Implementing a program of cyclical scheduling of nursing personnel.


Preliminary report on the larval development of the horn fly, Haematobia irritans, in feces from cattle given fractions of a commercial preparation of Bacillus thuringiensis.


New and little-known Plagiorhynchid acanthocephala from Taiwan and the Pescadores Islands.


Dual infections of Aedes aegypti L. with Plasmodium gallinaceum Brumpt and West Nile virus.


The excitatory synaptic action of climbing fibres on the Purkinje cells of the cerebellum. 1. A single climbing fibre makes an extraordinarily extensive synaptic contact with the dendrites of a Purkinje cell. Investigation of this synaptic mechanism in the cerebellum of the cat has been based on the discovery by Szentagothai & Rajkovits (1959) that the climbing fibres have their cells of origin in the contralateral inferior olive.2. Stimulation in the accessory olive selectively excites fibres that have a powerful synaptic excitatory action on Purkinje cells in the contralateral vermis, evoking a repetitive spike discharge of 5-7 msec duration. Almost invariably this response had an all-or-nothing character. In every respect it corresponds with the synaptic action that is to be expected from climbing fibres.3. Intracellular recording from Purkinje cells reveals that this climbing fibre stimulation evokes a large unitary depolarization with an initial spike and later partial spike responses superimposed on a sustained depolarization.4. Typical climbing fibre responses can be excited, but in a much less selective manner, by stimulation of the olive-cerebellar pathway in the region of the fastigial nucleus, there being often a preceding antidromic spike potential of the Purkinje cell under observation.5. Impaled Purkinje cells rapidly deteriorate with loss of all spike discharge, the climbing fibre response being then reduced to an excitatory post-synaptic potential. This potential shows that stimulation of the inferior olive may evoke two or more discharges at about 2 msec intervals in the same climbing fibre. The complexity of neuronal connexions in the inferior olive is also indicated by the considerable latency range in responses.6. A further complication is that, with stimulation in the region of the fastigial nucleus, the initial direct climbing fibre response is often followed by a reflex discharge, presumably from the inferior olive, which resembles the responses produced by inferior olive stimulation in being often repetitive.7. Typical climbing fibre responses have been evoked by peripheral nerve stimulation and frequently occur spontaneously.8. An account is given of the way in which the responses evoked by climbing fibres in the individual Purkinje cells can account for the potential fields that an inferior olive stimulus evokes on the surface and through the depth of the cerebellar cortex.9. By the application of currents through the recording intracellular electrode it has been possible to effect large changes in the excitatory post-synaptic potential produced by a climbing fibre, it being diminished and even reversed with depolarizing currents and greatly increased by hyperpolarizing currents.


The composition f human saliva secreted in response to a gustatory stimulus and to pilocaprine. 1. The composition of human saliva secreted in response to sour lemon drops (S.L.D.), and pilocarpine, was studied.2. At a given flow rate, pilocarpine-stimulated submandibular and parotid saliva contained less sodium and potassium and an equivalent amount of inorganic phosphate, and parotid saliva also contained more calcium and protein than did the corresponding types of S.L.D.-stimulated saliva.3. Prolonged S.L.D. stimulation did not cause a depletion in the protein concentration of either parotid or submandibular saliva and neither this procedure nor pilocarpine stimulation altered the proportions of the different proteins secreted.4. Pilocarpine was judged to be an inadequate substitute for more physiological, gustatory stimuli.


Mental age regression induced by lysergic acid diethylamide.


Inhibition of concept attainent in schizophrenics and in normals as a consequence of a set imposed by initial organization of data.


Standardization of physical fitness tests.


Assay of the autohumoral antibody that neutralizes the vitamin B12 combining site of intrinsic factor in serum from patients with pernicious anemia.


A riboflavin assay suitable for clinical use and nutritional surveys.


Dietary appraisal. Problems in processing dietary data.


Folic acid deficiency secondary to iron deficiency in man. Remission with iron therapy and a diet low in folic acid.


Effect of interchanging bread and sucrose as main source of carbohydrate in a low fat diet on the serum cholesterol levels of healthy volunteer subjects.


Effect of interchanging bread and sucrose as main source of carbohydrate in a low fat diet on the glucose tolerance curve of healthy volunteer subjects.


Growth of infants and preschool children in St. Christopher-Nevis-Anguilla, West Indies.


Nutritional anemias--WHO research program. Early developments and progress report of collaborative studies.


Systemic chromoblastomycosis due to Hormodendrum dermatitidis (Kano) Conant. Report of the first case in Taiwan.


Squamous papillomas of the uterine cervix. Review of the literature and report of a giant papillary carcinoma.


The serologic specificity (blood factor) C of the A-B-O blood groups. Theoretical implications and practical applications.


Studies on infectious mononucleosis. IV. Changes in the granulocytic series.


Three-hour prothrombin consumption test in siliconized glass. Diagnostic value in first-phase coagulation defects.


Effect of medium on anaerobic variations in sensitivity of staphylococci to the penicillins.


Recorder curve integration for quantitating trace metals in serum by atomic absorption spectroscopy.


A thin-layer chromatographic screening test for the detection of users of morphine or heroin.


An automated procedure for the determination of sweat electrolytes.


An automated system for the fluorometric determination of serum lactate dehydrogenase. Its adaptability to an automated system for the simultaneous determination of both serum lactate dehydrogenase and serum glutamic oxaloacetic transaminase.


Correlation of serum enzymes and morphologic alterations of the liver, with special reference to serum guanase and ornithine carbamyl transferase.


Isolation of Histoplasma capsulatum from bats in the United States.


A method for the continuous analysis of sulfobromophthalein in laboratory animals.


Smoking habits and survival of lung cancer patients. Application of the temporary expectation of life as a measure of survival.


Studies on eosinophilic meningitis. 2. Experimental infection of shrimp and crabs with Angiostrongylus cantonensis.


Use of ether treated influenza virus for antibody titration by means of the photometric ACU method.


Clinical, antigenic and prophylactic effects of an inactivated measles virus vaccine.


A color test for the measurement of antibody to the non-acid-forming human Mycoplasma species.


Arbovirus hemagglutinin-inhibition in avian sera: inactivation with protamine sulfate.


Trachoma in Punjabi Indians in British Columbia: a prevalence study with comparisons to India.


A new classification of diabetic retinopathy.


The effect of osmotic agents employed before cataract extraction. On the position of the vitreous following removal of the lens.


The congenital rubella syndrome with virus recovery. Ocular pathology and literature review.


The urinary excretion of vanilmandelic acid (VMA) and homovanillic acid (hva) in children with retinoblastoma.


Findings in the peripheral fundi of patients with retinoblastoma.


Cortical blindness in congenital hydrocephalus.


The ocular pathology of Behçet's disease.


A clinical evaluation of the electronic applanation tonometer.


Topical epinephrine in glaucoma suspects.


Preliminary observations on the use of new concentrations of echothiophate iodide in the treatment of glaucoma.


Schiøtz tonometry with an air bubble in the eye.


The electroretinogram after section of the optic nerve in man.


Chronic orbital myositis. Its diagnostic difficulties and pathology.


Surgery for ectropion.

Surgery for ectropion.


Experiments in scleral buckling. VI. Chamlin's scleral infolding evaluation with related procedures.


Management of errors of refraction with echothiophate iodide. A preliminary report.


Severe trauma following repair of retinal detachment. Report of three cases.


Calcifying orbital hemangioendothelioma.


Hemangioma of the lacrimal gland. Report of a case.


Improved telescopic loupe.

Improved telescopic loupe.


Double-purpose forceps. For cataract surgery.


Delayed hepatitis and cirrhosis in weanling rats following a single small dose of the senecio alkaloid, lasiocarpine.


Early hepatic parenchymal changes induced in the rat by aflatoxin B1.


Experimental pine pollen granulomatous pneumonia in the rat.


Ultrastructural renal alterations following tannic acid administration to rabbits.


Morphologic effects of folic acid and vitamin B12 on the jejunal lesion of tropical sprue.


Skeletal muscle structure in endemic cretinism.


Compensatory renal enlargement. Hypertrophy versus hyperplasia.


Ultrastructure of membranes in denervation atrophy.


Zn in yeast D-glyceraldehyde-3-phosphate dehydrogenase.


Ultrastructure and histochemical studies in a case of progressive dementia and its relationship to protein metabolism.


The stimulation of amino acid incorporation in a mammalian system with phenobarbital, 3-methylcholanthrene and cycloheximide.


Substrate specificity in pectin synthesis.


Incorporation of D-xylose-C14 into glycoprotein by particles from hen oviduct.


Incorporation of D-galactose into liver microsomal protein in vitro.


A reversible reaction between the epsilon-amino groups of cytochrome c and salicylaldehyde.


The role of water in deoxygenated hemoglobin solutions.


Optical rotatory dispersion of flavin nucleotides.


Bacterial photophosphorylation in the absence of added nucleotide. A second intermediate stage of energy transfer in light-induced formation of ATP.


The effects of palmitylcarnitine on hepatic fatty acid synthesis and on acetyl CoA carboxylase activity.


Relief of palmityl CoA inhibition of citrate synthase by long-chain acylcarnitine derivatives.


Screening data from the Cancer Chemotherapy National Service Centescreening laboratories. 34. Plant extracts.


Neonatal cardiorespiratory research in Europe: current status.


Hereditary ectodermal dysplasia. Symptoms, inheritance patterns, differential diagnosis, management.


Refrigerator entrapment.

Refrigerator entrapment.


Earlier puberty. Its social and psychologic consequences.


Safety swim rules.

Safety swim rules.


Health education and the adolescent.

Health education and the adolescent.


Ellis-van Creveld syndrome.

Ellis-van Creveld syndrome.


An unusual example of ectodermal agenesis.


Acute glomerulonephritis with minimal urinary findings.


The grand round. Is there a coefficient of utility (Cu)?


Government health care in Canada.

Government health care in Canada.


Radiation. Benefits and hazards.

Radiation. Benefits and hazards.


The service manager. Innovation for nursing and health organizations.


Disaster planning for the clinical laboratory. 2.


Chromatographic separation and quantitative estimation of isomeric dinitrobenzenes.


Genetic mechanisms of aging.

Genetic mechanisms of aging.


Cautiousness in advanced age.

Cautiousness in advanced age.


Characteristics of mentally ill aged: A comparison of patients with psychogenic disorders and patients with organic brain syndromes.


The role of genetic factors in the aging process.


Ability scores as related to age among female job applicants.


Aging in germ-free mice: life tables and lesions observed at natural death.


Visual short-term memory as a function of age, rate of presentation, and schedule of presentation.


The Stockton Geriatric Rating Scale.

The Stockton Geriatric Rating Scale.


Longevity and gross pathology at death in 22 inbred mouse strains.


Interests of doctoral students in clinical psychology in work with older adults.


Free fatty acid responses in the aged individual during performance of learning tasks.


The phosphoglucomutase, phosphoglyceric acid mutase, and phosphomannose isomerase activities of arterial tissue in individuals of various ages.


Preparation for death in old age.

Preparation for death in old age.


Variations of forearm blood flow with age and sex.


Changes in blood cellular and protein components during aging.


Objective and subjective economic disengagement among older rural and urban men.


X-ray-induced glomerulosclerosis in rats: modification of lesion by food restriction, uninephrectomy, and age.


Variations in extractable collagen of bone and skin with age and growth.


Attitudes toward old people: a re-analysis of the item-validity of the stereotype scale.


Personality stereotypes of aging.

Personality stereotypes of aging.


Personality characteristics of successful older leaders.


Canine heartworms in experimental cardiac and pulmonary surgery.


Effect of corticosteroid on the acidosis of prolonged cardiopulmonary bypass.


The effect of abdominal aortography on proximal renal tubule function.


The problem of the aged in society.

The problem of the aged in society.


The medical care of the elderly.

The medical care of the elderly.


The problem of the aged in society--a general practitioner's view.


Psychiatric admissions from nine Sydney municipalities or shires.


Preliminary communication heterograft aortic valve transplantation for human valve disease.


Thromboembolism and oral contraceptives.


Delayed convalescence after initial cardiac infarction.


Sickness and accidents amongst the Australian olympic team in Tokyo, 1964.


Reports from Australian virus laboratories.


Modified technic of inguinal lymphadenectomy.


Intravaginal use of potassium permanganate as an abortifacient: the error in diagnosis.


Ultrasonic echoscopic examination in late pregnancy.


Multicentric Bowen's disease of the vulva. Report of a case.


Fate of compatible adult erythrocytes in the fetal peritoneal cavity.


Amniotic fluid spectrophotometry.

Amniotic fluid spectrophotometry.


Hormonal therapy for control of the incompetent os of pregnancy.


Treatment of the incompetent cervix with the Hodge pessary.


Meatal ulceration following neonatal circumcision.


Efect of a potent non-narcotic analgesic agnt (pentazocine) on uterine contrctility and fetal heart rate.


Sarcoma of the round lgament. Report of a case.


Pregnancy and exstrophy of the urinary bladder.


Clinical aspects of the Stein-Leventhal syndrome.


Treatment of gynecologic wound complications.


Squamous-cell carcinoma of the cervix with higher uterine involvement.


Once a section, always a trial of labor?


Influence of adrenalectomy on homologous disease.


Effect of dimethylsulfoxide on isolated-innervated skeletal, smooth, and cardiac muscle.


Insulin hypoglycemia enhanced by beta adrenergic blockade.


Exacerbation and transformation of allergic encephalomyelitis by pertussis vaccine.


Metabolism of I-131 by human thyroid tissue in organ culture.


Collagen and hexosamine changes in subcutaneous granuloma irradiated locally with a Co60 source.


Measurement of non-crystalline calcium phosphate in bone mineral.


Studies on the interaction between gamma-aminobutyric acid and tubocurarine on intraventricular injection in cats.


Influence of restricted food intake on cardiac glycogen mobility.


Growth response of Lactobacillus casei (ATCC 7469) to riboflavin, FMN, and FAD.


Production of urea nitrogen and creatinine in chronic azotemia and effect of hemodialysis.


Effect of cortisone on metabolism of cystine in regenerating wound tissue.


Unresponsiveness of the adult toad to thyroxine administration.


Activation of factors XII (Hageman) and XI (PTA) by skin contact.


Efficacy of N6-(2-hydroxyethyl)adenine against Coe virus infection in mice.


Mechanism of gelatin inhibition of reticuloendothelial function.


Alkaline phosphatases of the chick. Partial characterization of the tissue isozymes.


Effect of estrogen and progesterone on mammary gland DNA and feed intake in hypophysectomized female rats.


Postnatal changes in the cardiac ventricles of the pig.


Evidence for the existence of human growth hormone-ribonucleic acid complex in the pituitary.


Separation of human growth hormone into two components--GHA and GHB.


Immunologic isolation of human intrinsic factor.


Myocardial depressant factor in plasma from cats in irreversible post-oligemic shock.


Antigen production in hyperoxic organ cultures.


Methods for obtaining the venous outflow from the hypothalamus and hypophysis.


Effects of serum uric acid and parotid flow rate on concentration and secretion rate of uric acid in parotid fluid.


Base composition of ribosomal ribonucleic acid in newborn and adult rat brain.


The influence of calcium on the binding of antithrombin by antithrombin inhibitor.


Hydroxocobalamin. VI. Comparison of intestinal absorption in man of large doses of hydroxocobalamin and cyanocobalamin.


Effect of testosterone on the involution of male rats' mammary glands.


Food intake and activity of rats with rostral hypothalamic lesions.


Histobacteriology of the genus Pasteurella II. Specificity and stability of the various species of Pasteurella.


Acute effect of metal ions on uptake of alpha-aminooisobutyric acid (AIB) by rat parathyroids in vivo.


Studies of the effect of dimethylsulfoxide on permeability of dermal connective tissue.


Effects of d-amphetamine on plasma and tissue electrolyte concentrations of aggregated and of hyperthyroid mice.


Precipitin and neutralizing antibody response elicited by Crotalus atrox venom-antivenom precipitate.


Effect of hydrocortisone feeding on concentration of free fatty acids and other lipids of rabbit sera.


Insulin antibodies: partial characterization by gel filtration.


Influence of deuteration on circulating antibody levels in the mouse.


Scleroderma: dermal amino acid composition with particular reference to hydroxyproline.


Effects of oxygen on determination of thiocholine by 5,5'-dithiobis(2-nitrobenzoic acid).


Studies of hamycin on inflammation and related mechanism.


Micromethod for determination of specific gravity of tissues.


Lack of effect of insulin and blood fractions on glucose-ATP phosphotransferases.


Stimulation by insulin of protein synthesis in isolated fat cells.


Entry of L-phenylalanine-C14 into brain and cerebrospinal fluid.


Studies on the intracellular distribution and tissue binding of dihydromorphine-7,8-H3 in the rat.


Effects of diuretic agents on serum and tissue electrolytes in rats.


Iron and transferrin distribution and turnover in iron-overloaded rabbits.


Absorption of 14C triolein in the bile fistula rat.


Alteration of hepatic protein synthesis in acute uremia.


Studies in contact dermatitis. XVII. Spectacle frames.


Fatal motorcycle and motorscooter accidents.


Studies in contact dermatitis. XVI. Contraceptives.


Oral ulceration.

Oral ulceration.


The role of immunological mechanisms in inflammation.


Glossodynia--a psychosomatic problem.

Glossodynia--a psychosomatic problem.


Virus warts. A statistical survey.

Virus warts. A statistical survey.


Chlorpromazine purple people.

Chlorpromazine purple people.


Extreme capsule in the dog: myeloarchitectonics.


Myeloarchitectonics of stria terminalis in the dog.


Stria medullaris of the thalamus in the dog.


Salivation and instrumental responding to an instrumental CS pretrained using the classical conditioning paradigm.


Retention of the avoidance reflex after prefrontal lobectomy in cats.


The effect of food reinforcement on the level of alimentary excitation.


The control of the territory by Lasius fuliginosus Latr.


The myeloarchitectonics of the hypothalamus in the dog. I. The anterior nuclei.


Double contrast radiographic examinations of the upper gastrointestinal tract using barium spray technic.


Percutaneous transhepatic cinécholangiography.


Distribution of serotonin (5-hydroxytryptamine) in the human gastroinetestinal tract.


Reassessment of the fate of esophageal varices following portal decompression in cirrhosis.


Provocation of serum enzyme activity in cholecystectomized patients given opiates.


Villous adenoma of the colon. Report of an unusual case in the cecum.


Recurrence of regional ileitis in a blind loop after ileocolostomy.


Ultra sound enhanced local anesthesia.

Ultra sound enhanced local anesthesia.


Use of the Moore Vitallium prosthesis in osteoarthritis of the hip.


The ball and socket hip prosthesis.

The ball and socket hip prosthesis.


Phospholipid synthesis in the large pulmonary alveolar cell. Its relation to lung surfactants.


Experimental amyloidosis. Role of antigenicity and rapid induction.


The pathology of cerebral edema associated with gliomas in man. Report based on ten biopsies.


Loss of selective injurious vascular response to cadmium in regenerated blood vessels of testis.


Occurrence of caterpillar nuclei within normal immature and normal appearing and altered mature heart muscle cells and the evolution of Anitschkow cells from the latter.


Desalting of proteins, peptides, and amino acids on polyacrylamide gel.


Measurement by starch-gel electrophoresis of enthalpy change during reversible dissociation of groundnut protein.


High-resolution disc electrophoresis of histones. I. An improved method.


High-resolution disc electrophoresis of histones. II. Application to whole and fractionated preparations from various laboratories.


A one-step ultramicro method for the assay of intestinal disaccharidases.


Separation of cystine, penicillamine disulfide and cysteine-penicillamine mixed disulfide by automatic amino acid analysis.


On the determination of galactosamine-uronic acid.


A simple method for determining the labeling efficiency of fluorescein isothiocyanate products.


Thin-layer chromatographic separation of DNS-amino acids.


A fluorometric assay for glutathione.

A fluorometric assay for glutathione.


Measurement of the radioactivity in carbon-14 and tritium-labeled proteins that have been separated by disk electrophoresis.


Separation of the components in collagen by preparative starch-gel electrophoresis.


A new method of sample application for column chromatography of amino acids.


Purity of crystalline protohemin IX.

Purity of crystalline protohemin IX.


An automatic masking assembly for multiple-cell velocity experiments with the UV optics of the ultracentrifuge.


Simplification of oxygen-flask combustion procedure for preparation of samples for liquid scintillation counting.


Mono- and bis(2-hydroxyethyl)imino-tris(hydroxymethyl)-methane, "mono-tris" and "bis-tris": new buffer bases with pKa t 7.83 and 6.46.


Reduction of light scatter in fluorometry by the use of horizontally polarized excitation.


Structural changes in microsomal suspensions. IV. Effects of ions.


Mitochondrial particles resolved for ion translocation. II. Extraction of cofactors and restoration of activity.


Correlation of spectroscopic observations on polyenes with ripening of tomato fruits.


Purification and characterization of four Bence-Jones proteins.


The biosynthesis of the methyl groups of choline in Ochromonas malhamensis.


Intestinal disaccharidase activities in the growing germfree and conventional rats.


Studies on Taka-amylase A under high pressure. IV. Influence of initial concentration of enzyme upon pressure inactivation and reactivation.


Hemosiderin. 3. Determination of sialic acid and the components of a carbohydrate complex.


Hemosiderin. IV. Effect of iron on the determination of bound sialic acid and on deoxyribose.


In vivo incorporation of [2-14-C]-mevalonate into dolichol of rabbit and pig liver.


Separation and properties of potato invertase and invertase inhibitor.


New reactions of dextransucrase: alpha-D-glucosyl transfers to and from the anomeric sites of lactulose and fructose.


Phenylalanine conversion to tyrosine by the human fetal liver.


Electrophoretically identical histones from ribosomes and chromosomes of chicken liver.


The oxygen Bohr effect in mouse hemoglobin.


Cytochrome c redox reactions induced by photochemical system 1 in sonicated preparations of Anabaena cylindrica.


Electrophoretic studies of light-induced charge in spinach chloroplasts.


Comparative studies of cytochrome c redox reactions by photochemical lamellar preparations obtained from blue-green, red and green algae, and spinach chloroplasts.


New synthesis of poly-L-glutamic acid and poly-L-glutamyl proteins.


Suggestibility and national character.

Suggestibility and national character.


Enhancement of succinate oxidation in lung and liver mitochondria of tuberculous mice.


Peace of mind and hypnosis.

Peace of mind and hypnosis.


Hypnoanalysis: an effective approach to marital problems.


Connective tissue-reactions in the cervix uteri. Myometrial changes under the influence of estrogenic and androgenic hormones and during pregnancy.


Streptococcal bacteriophages with special reference to phages acting on group A streptococci.


The GC serum group system and its application in forensic medicine.


Some clinicobacteriological studies in a maternity unit.


Vestibulo-spinal reflexes. Clinical and experimental studies using the stepping test.


Facial pain. A clinical study with special reference to the symptomatology, aetiology and surgical therapy.


Weeverfish stings. Frequency, occurrence, clinical course, treatment and studies on the venom apparatus of the weeverfish, the nature of the toxin and immunological aspects.


The kidney in diabetes mellitus. A clinical and histological investigation based on renal biopsy material.


Hereditary galactosaemia measurements of galactose-1-phosphate-uridyltransferase with reference to diagnosis and mode of inheritance.


Gastric cytology with special reference to the diagnosis of malignant disease.


Lung function studies in patients with lung and heart diseases and obesity.


The course of psychiatric diseases in children.


The excretion of some carbohydrates in the urine during pregnancy and lactation.


Equipment lists and suggested layouts in new USPHS dietary planning guide.


How housekeeping services were improved in a long-term unit.


A serious hospital deficiency.

A serious hospital deficiency.


A small proprietary hospital closes its doors.


Hospitals aid Korean health program.

Hospitals aid Korean health program.


"At risk" hospital personnel singled out by smallpox and tetanus survey.


An outsider's view of the flaws in the health care economy.


Preparing medical record data for computer processing.


Pharmacy service in the smaller hospital: a survey of 12 Minnesota facilities.


Building an effective training program for laundry employees.


Who keeps the kitchen clean?

Who keeps the kitchen clean?


Intra-ocular tension in normal eye.

Intra-ocular tension in normal eye.


Gonio-sweeping with vitreous suction in malignant glaucoma.


Glaucoma screening.

Glaucoma screening.


Two pillar iridencleisodialysis with cataract extraction in cataract with glaucoma.


Cataract and iridencleisis--one stage operation.


Different techniques of iridencleisis and their value in glaucoma. (A study of 60 cases).


Cataract extraction following glaucoma surgery. (Assessment of 100 cases).


Experience with the Posner-Inglima tonometer.


Analysis of the monoethylenic fatty acids of rapeseed oil by open tubular gas chromatography.


The reaction of picric acid with epoxides. II. The detection of epoxides in heated oils.


Polymorphism of cocoa butter.

Polymorphism of cocoa butter.


New nonionic detergents based on epoxidized oils. II.


Epoxidation of methyl linoleate. II. The two isomers of methyl 9, 10: 12, 13-diepoxystearate.


Isomeric linear phenylalkanes and sodium alkylbenzenesulfonates.


Polymorphism of palmitoyl and stearoyl dioleins.


Lipids in wheat from various classes and varieties.


The importance of the District of Columbia optometry bill.


Cyclic fatty acids: removal of aromatic acids formed during hydrogenation.


Operation uplift.

Operation uplift.


Protection and promotion.

Protection and promotion.


Optical compensation for heterophoria.

Optical compensation for heterophoria.


Relationship of perceptual and visual skills with reading accuracy and comprehension.


Method of producing permanent wettability on plastic contact lenses.


Economic survey. 4.

Economic survey. 4.


Revision of the Van Deemter theory for diffusion and pressure drop in the gas phase.


Separation of malonic acid esters by gas chromatography.


Gas chromatography using cholesteryl ester liquid phases.


Separation of cis- and trans-alkyl substituted oxiranes and aziridines.


The use of Sephadex in the chromatography of thyroxine-containing compounds: a critique.


Column chromatography of nucleotides over thymidylate-cellulose.


Studies on grass lignins. I. Separation and quantitative determination of p-hydroxybenzaldehyde, vanillin and syringaldehyde by thin-layer chromatography.


Quantitative thin-layer chromatography of free and conjugated cholic acid in human bile and duodenal contents.


Reversed-phase paper chromatography of the rare earths, thorium and uranium using methylenebis(di-n-hexylphosphine oxide) as the stationary phase.


Combination of temperature gradient with gel electrophoresis and its applications to analysis of collagen-gelatin transition.


Chromatography on ion exchange papers. XIX. Ion exchange of metal ions in thiocyanic acid.


Separation of DDT and related compounds on a silicic acid column using gradient elution.


Low temperature discontinuity of retention times with Carbowax 20-M.


Permanent records of thin-layer chromatograms on transparent paper.


Separation of closely related steroids by an improved technique for continuous development of thin-layer chromatograms.


Response of steroids to sulfuric acid in thin-layer chromatography.


An improved paper chromatographic separation on 4 ribonucleosides, their 3'(2')-phosphates and 3'(2'),5'-diphosphates.


Conditioned stimulus duration and delay of reward as variables in a lever pulling situation.


The response of the human newborn to visual movement.


Effects of correlated reinforcement on response amplitude in children.


Percentage of occurrence of response members, associative strength, and competition in paired-associate learning in children.


Feedback and infralogical strategies in invariant area conceptulization.


Cross-modal transfer and improvement of form discrimination.


A semiautomatic procedure for measuring changes in position.


Chondrosarcoma.

Chondrosarcoma.


Pelvic arteriovenous fistula. Report of a case and management.


Intraperitoneal versus lesser peritoneal sac air in rupture of a hollow viscus. The falciform ligament sign.


The don'ts of fracture care.

The don'ts of fracture care.


Some aspects of hip dysplasia and coxa plana in dogs.


Into the future.

Into the future.


Ambulance and emergency department of services in Maine.


Arthroplasty of the dysplastic canine hip joint. An experimental study with special reference to histochemistry and oxytetracycline bone labelling.


Editorial comment: veterinary education in the clinical years.


Gastrin.

Gastrin.


Problems of maternal rubella.

Problems of maternal rubella.


Pre-glaucoma and its diagnosis.

Pre-glaucoma and its diagnosis.


Diaphragmatic hernia.

Diaphragmatic hernia.


Clothing dermatitis.

Clothing dermatitis.


Radiology of the urinary system.

Radiology of the urinary system.


Wound healing and fistula-in-ano.

Wound healing and fistula-in-ano.


Biochemical research in schizophrenia.

Biochemical research in schizophrenia.


Circulating cancer cells in surgery.

Circulating cancer cells in surgery.


Host-finding by mosquitoes.

Host-finding by mosquitoes.


Present-day indications for surgical treatment of varicose veins.


The specialist and the health of the university.


Evaluation of velopharyngeal competence after primary repair of the palate.


Thirty-three years' perspective of urinary tract infections.


Genital anomalies associated with solitary kidney.


Socially unacceptable language in a hospital for the mentally retarded.


A simple mouth-held writing device for use with cerebral palsy patients.


[A pilot study in evaluating camping experiences for the mentally retarded].


Operant conditioning: an application to behavioral problems in groups.


Criteria for admission to institutions for the mentally retarded.


Parent responses to a mentally defective child.


Projection of program requirements for the mentally retarded: approaches and problems.


Orbital fractures.

Orbital fractures.


The syndrome of ankyloglossia superior.

The syndrome of ankyloglossia superior.


Retroperitoneal fibrosis: a confusing syndrome.


Lung scintiphotography.

Lung scintiphotography.


Partial anomalous pulmonary venous return. A hypoplastic right lung to the inferior vena cava (Scimitar syndrome).


Detection and localization of intracranial lesions.


Discovering small bowel tumors.

Discovering small bowel tumors.


Euthanasia--to set you straight.

Euthanasia--to set you straight.


The viewing box. Carcinoid of the duodenum.


The prevention and treatment of knee injuries in athletes.


Neurological conference. Associated problems with parkinsonism.


Prenatal care and delivery of a diabetic patient.


Antibacterial management of infections due to gram-negative bacilli. IV. Principles of management, empiric therapy, and some infections of the respiratory tract.


A new instrument for obtaining bone marrow for biopsy.


Space laboratories for research in biology and medicine. Promising developments.


Diagnosis by gastrocamera.

Diagnosis by gastrocamera.


Stenosing small-bowel ulceration.

Stenosing small-bowel ulceration.


Pulmonary emphysema and the heart. I.

Pulmonary emphysema and the heart. I.


Massive lung cavitation, complicated by jaundice and splenomegaly.


The M.D. and the V.M.D.

The M.D. and the V.M.D.


Contamination control: surgical and industrial.


Ethanolamine metabolism in plant tissues. Ethanolamine is readily metabolized by oat, pea, wheat, apple and carrot tissue preparations. Ethanolamine-1,2 (14)C was incorporated into the lipid fraction, and (14)C activity was distributed in the organic acid, sugar, acid volatile, carbon dioxide and insoluble residue fractions. The distribution varied with the particular tissue. Incorporation into the lipid fraction occurred in tissue homogenates in the absence of ATP by a Ca(++) activated system similar to that reported for animal preparations. The initial step in ethanolamine oxidation involves an amine oxidase. Glycolaldehyde and glyoxylic acid are metabolic intermediates, the former in the conversion of ethanolamine to carbon dioxide. No evidence was obtained for the operation of an ethanolamine transaminase or for the involvement of phosphorylated intermediates in the conversion of ethanolamine to carbon dioxide.


Intracellular localization of individual dehydrogenases in frozen plant tissues by means of specific substrates and coenzymes.


Reduction of nitrate and nitrite by subcellular preparations of Anabaena cylindrica. I. Reduction of nitrite to ammonia. Reduction of nitrite by cell-free preparations of Anabaena cylindrica in the dark has been investigated. Nitrite-reducing activity was recovered in a supernatant fraction. The nitrite reductase system was partially purified by column chromatography on Sephadex G-75. NADPH could serve as an H-donor. NADH was completely inactive. The reduction required ferredoxin which mediated the transfer of electrons from NADPH to nitrite. Ferredoxin was successfully replaced with methyl viologen, benzyl viologen and diquat. The nitrite-reducing activity was inhibited by KCN, and by 2,4-dinitrophenol and arsenate at higher concentrations. The extent of nitrite reduction by NADPH was dependent on the oxidation-reduction states of NADP and ferredoxin.


A vessel for the replicate culture of cells in monolayer.


Oriented embedding of biological materials and accurate localization for ultrathin sectioning.


Preparation of tungsten microneedles with a propane torch.


Coronary artery visualization and coronary surgery--a word of caution.


Heart sounds and murmurs in pregnancy.

Heart sounds and murmurs in pregnancy.


Alveolar-arterial oxygen tension gradients in cirrhosis of the liver. Further evidence of existing pulmonary arteriovenous shunting.


Endocardial fibroelastosis in American Negro children: a distinct entity?


Fiber optics for continuous in vivo monitoring of oxygen saturation.


The significance of postmortem coronary arterial perfusion studies.


The low-frequency response of electrocardiographs, a frequent source of recording errors.


High-fidelity electrocardiography: effects of induced localized myocardial injury in the dog.


The simultaneous occurrence of ventricular pre-excitation, left bundle branch block, and delayed A-V conduction.


Calcification of the aorta and main vessels in rheumatoid arthritis.


Mycardial infarct and sudden coronary heart death in relation to coronary occlusion and collateral circulation.


Platelet thrombosis in experimental renal papillary necrosis.


Antianginal drugs. V. Monoamine oxidase (MAO) inhibitors.


Prolonged obstructive jaundice in infancy. II. Neonatal hepatitis.


Sudden death and the oxygen-conserving reflex.


High-altitude pulmonary hypertension.

High-altitude pulmonary hypertension.


The second heart sound in pulmonary embolism and pulmonary hypertension.


Trace elements in public water supplies and dental caries.


Progress in control of cancer of the uterus.


Impact of economic development on health patterns in Egypt.


Narcolepsy following injury by electric current. Three cases, with a commentary on the contributory role of diabetogenic hyperinsulism.


Goodbye, general practice.

Goodbye, general practice.


Thermal responses of man during rest and exercise in a helium oxygen environment.


Concentrations of carbon monoxide at traffic intersections in Dayton, Ohio.


The respiratory response of guinea pigs to histamine aerosol.


Cigarette smoke and ciliastasis. Effect of varying composition of smoke.


Environment in relation to coronary artery disease.


Smoking in medical students. A survey of attitudes, information, and smoking habits.


Environmental chamber for the study of respiratory stress in small animals.


Cigarette smoking and medical attendance prior to death.


Skin tests in blastomycosis. A comparative study.


Allergic reactions following immunization procedures.


The problem of radioactive contamination of the skin.


Toxoplasma gondii from Toxocara cati.

Toxoplasma gondii from Toxocara cati.


Eye movement recordings in neurological disorders.


Visual acuity in strabismic amblyopia under monocular and binocular conditions.


Isoflurophate versus glasses in evaluating the accommodative element in esotropia.


Corneal damage in hydroquinone workers. A clinicopathologic study.


Unilateral hypoplasia of the optic nerve.


Angioid streaks in Ehlers-Danlos syndrome.


Ocular complications of hemophilia.

Ocular complications of hemophilia.


Endogenous intraocular aspergillosis.

Endogenous intraocular aspergillosis.


Dysthyroid ocular myopathy. Anatomy, histology, and electron microscopy.


Polygeline as a vitreous substitute. I. Observations in rabbits.


Mechanisms of the host response in the eye. II. Variations in ocular disease produced by several different antigens.


Izozymes of malate dehydrogenase in urediospores of Puccinia recondita Rob. ex Desm.


Gestational blood glucose levels in normal and potentially diabetic women related to the birth weight of their infants.


Sulfated insulin in mild, moderate, severe and insulin-resistant diabetes mellitus.


Studies of the metabolic effects of acute insulin deficiency. I. Mechanism of impairment of hepatic fatty acid and protein synthesis.


Hepatic glucose phosphototransferases. Variations among species.


Cerebrospinal fluid lipids in diabetes mellitus.


Effect of insulin on the formation of glycogen by the mouse diaphragm in the presence and absence of glucose. A preliminary report.


Gynecologic surgery in geriatric patients.


Sex distribution and frequency of diabetic concomitants of complications.


Treatment of obstructive bronchial diseases. Periarterial sympathectomy with removal of carotid body.


A follow-up survey of predominantly older amputees.


Correction of malnutrition in the aged. Comparative efficacy of an anabolic hormone and an enzyme vitamin complex.


Adhesive platelets and myocardial infarction.


Value of routine sigmoidoscopic examination.


Economics and geriatric rehabilitation.

Economics and geriatric rehabilitation.


Dynamic maturity for purposeful living.

Dynamic maturity for purposeful living.


Psychiatric day treatment in the general hospital.


The families of psychiatric in-patients and day patients.


Culture and mental disorder: a study in an Indian mental hospital.


Paranoia and masochism: stages on the road to despair.


Re-socialization: undoing the damage.

Re-socialization: undoing the damage.


Reform in the mental hospital: an eight year follow-up.


General practice in Australia.

General practice in Australia.


General practice in New Zealand.

General practice in New Zealand.


General practice in Great Britain.

General practice in Great Britain.


General practice and the White Paper.

General practice and the White Paper.


Conditioning in enuresis--its efficacy and the factors involved.


Goodpasture's syndrome--a case report.

Goodpasture's syndrome--a case report.


Transport mechanisms in classical renal tubular acidosis.


Concentrations of inorganic ions in guinea-pig inner ear fluids. I. Concentrations of potassium and sodium in cochlear and utricular endolymph.


Trans-sphenoidal hypophysectomy. (A trans-septal extranasal approach).


Secondary post-tonsillectomy haemorrhage.


Supraglottic laryngectomy and functional neck dissection.


Partial laryngectomy.

Partial laryngectomy.


Forty-two years survival after laryngectomy.


Concretion in the ear.

Concretion in the ear.


Supraglottic laryngectomy and functional neck dissection. Discussion on paper by Professor Bocca.


Increased permeability of the capillaries of the rat heart to plasma albumin with asphyxiation and with perfusion. 1. Studies on the rate of efflux from the isolated perfused rat heart of plasma albumin conjugated with Evans Blue showed the conjugate to have penetrated extensively the extravascular compartment of the organ during a period of 2 min. This was confirmed by direct analysis of hearts for Evans Blue after perfusion.2. Exposure of the hearts to Evans Blue-albumin conjugate for 8 min in vivo showed no significant penetration of the interstitial space.3. With the isolated preparation inclusion of promethazine in the perfusing medium significantly diminished the rate of penetration of the extravascular compartment by the conjugate as did injection of the animals with either reserpine 2 days before, or bretylium immediately before the experiment.4. Penetration of the interstitial compartment in vivo could be induced by repeated asphyxiation. This penetration could also be diminished by promethazine but was not influenced by mepyramine maleate. The increased permeability of capillaries to plasma proteins can be readily demonstrated in the whole animal by detecting the leakage from the vasculature of the plasma proteins conjugated with a dye (Menkin & Menkin, 1930; Miles & Miles, 1952). Evans Blue, which has been widely used as a vascular marker because of the stability of its conjugate with plasma albumin, is the most suitable dye for this purpose. In the present work this method has been applied to the isolated rat heart, to determine if changes in capillary permeability occur in the perfused tissue. When plasma albumin conjugated with Evans Blue was used as a vascular marker in this preparation, its rate of clearance was very much less than that of erythrocytes, and the amount contained in the heart corresponded to a space approaching that occupied by extracellular markers such as raffinose and inulin. It was concluded, therefore, that there was a failure of a considerable number of the cardiac capillaries to retain the plasma albumin, and subsequent work was directed towards the identification of the condition that gives rise to these changes and the possible involvement of a permeability factor.A preliminary account of part of this work was given to the Physiologcal Society (Sutherland & Young, 1961).


Lingual mechanoreceptors activated by muscle twitch. 1. Two groups of mechanoreceptors in the tongue have been identified by recording afferent discharges in single nerve fibres dissected from the lingual nerves of anaesthetized cats. The group of superficially situated, rapidly adapting mechanoreceptors had faster conduction velocities than the presumed deeply situated, slowly adapting endings.2. Stretch of the tongue musculature did not prove to be as efficient in activating the endings as local deformation, although it was possible to excite some of the presumed deeply situated endings in this way.3. No mechanoreceptor fibres could be identified in filaments dissected from the hypoglossal nerves in the same experiments.4. Twitches of the tongue musculature produced by stimulation of the hypoglossal nerve were able to cause discharge of superficial and presumed deeply situated mechanoreceptors during the tension change.5. The timing of the discharges in response to twitch was such that it could account for the delay in synaptic potentials produced in hypoglossal motoneurones when supramaximal stimuli were applied to the hypoglossal nerve.6. The possible significance of mechanoreceptor discharges in reflex activation of tongue motor units is discussed.


Taste reception in the goat, sheep and calf. 1. The goat, sheep and calf have gustatory chemoreceptors which respond to salt, sweet, sour and bitter solutions, from which the afferent fibres pass centrally in the chorda tympani and glossopharyngeal nerves.2. Action potentials in the afferent gustatory nerves can also be detected when the tongue is irrigated with sodium bicarbonate, ethylene glycol, glycerine, and saccharine.3. Distilled water reduces the background activity in the isolated gustatory nerves, thus demonstrating the absence of fibres in ruminant ungulates of receptors which respond to stimulation by distilled water.4. The responses from the chemoreceptors, associated especially with the circumvallate papillae, are enhanced if the papillae are gently moved at the time of irrigation with sapid solutions.5. The chorda tympani is most responsive to salt and acid solutions while the glossopharyngeal is more responsive to sugars, quinine and acid.


Potentiometric measurement of membrane action potentials in frog muscle fibres. 1. A potentiometric method for recording membrane action potentials from small segments of isolated skeletal muscle fibres has been described.2. The value of the method in muscle physiology has been appraised by recording from fast and slow skeletal muscle fibres of the frog under a variety of conditions.3. Artifacts associated with this method have been described and their sources investigated.


The behaviour of a mammalian muscle during sinusoidal stretching. 1. Tension changes have been recorded in tetanized and passive cat soleus muscles during sinusoidal stretching at frequencies between 0.25 and 12.5 c/s.2. During sinusoidal stretching the muscle tension varied in an approximately sinusoidal manner. The tension record showed an angular advance on muscle length. A method of measuring mean angular advance in nonlinear systems is described.3. In tetanized muscle, resistance to lengthening increased with increasing frequency of stretching. With increasing frequency the elastic stiffness increased more rapidly than the viscous stiffness, so that at the higher frequencies the angular separation between length and tension became smaller.4. With increasing stretch amplitude the elastic stiffness of the tetanized muscle declined, and the mean angular advance of tension became larger.5. In passive muscle the viscous stiffness increased at the higher frequencies, and the mean angular advance of tension on length was correspondingly increased.6. The functional significance of these findings and their relation to the findings of other workers are discussed.


The localization of lysosomal enzymes in chromaffin tissue. 1. An homogenate of bovine adrenal medulla contains significant amounts of six acid hydrolases: acid ribonuclease, acid deoxyribonuclease, cathepsin, acid phosphatase, beta-glucuronidase and arylsulphatase. Most of the activity of each enzyme could be sedimented in the large-granule fraction at 242,000 g-min.2. Differential centrifugation indicated the presence of three populations of particles, which sedimented at slightly different rates; these are, in order of decreasing sedimentation rate, mitochondria, particles containing the acid hydrolases, and chromaffin granules.3. The three types of particle could be separated by ultracentrifuging the large-granule fraction in a sucrose density gradient. Most of the activity of each hydrolase was recovered in a layer intermediate between those formed by mitochondria and chromaffin granules.4. The large-granule fraction therefore contains particles which are defined by their enzyme content as lysosomes.5. Highly purified chromaffin granules, containing less than 5% of the activity of each acid hydrolase, were obtained from the gradient.


Rapid hydrogen ion uptake of rod outer segments and rhodopsin solutions on illumination. 1. Flash illumination of a suspension of frog rod outer segments or rhodopsin solution in contact with a platinum electrode produces a rapidly developing negative displacement of potential of the electrode (with respect to a reversible electrode).2. The amplitude of the potential change varies inversely with the H(+) buffering capacity of the medium. It is inferred that the response is due to an uptake of H(+) by the rod outer segments or rhodopsin, with the platinum surface acting as a pH electrode.3. Determination of the action spectrum shows that the response depends on the absorption of light by rhodopsin.4. In frog rods one acid-binding group with a pK of about 7.9 is produced for each molecule of rhodopsin bleached, consistent with a rhodopsin concentration in frog rods of 1.7 mM.5. It is suggested that the time course of the response with rhodopsin solutions reflects the kinetics of the conversion of metarhodopsin I to metarhodopsin II.6. A slower time course of voltage change observed for suspensions of outer segments is attributable to the time required for the diffusion of H(+) buffer out of the rods.


Water diuresis in the domestic fowl.

Water diuresis in the domestic fowl. 1. The exteriorization of ureters in domestic fowls allows the collection of urine uncontaminated by faeces.2. When water is administered by stomach tube to a fowl, water is stored in the crop, from which it is slowly released into the proventriculus.3. Immediately after water gavage, there is a sharp rise of urine flow accompanied by an increase in endogenous creatinine excretion and a fall in the osmolarity of the urine. This initial increase in urine flow is of short duration, and is followed by a normal curve of diuresis with a peak at about 90 min after hydration.4. The first rise in urine flow following the filling of the crop appears to be of reflex origin, as distension of the crop with paraffin produces an enhanced excretion of urine and of creatinine.5. Changes in urine flow and in creatinine excretion are closely correlated.6. After water deprivation for 18 hr, the urine is slightly hyptertonic to blood. The maximum urine concentration observed was of the order of 600 m-osmole/l.


Evidence that the secreting adrenal chromaffin cell releases catecholamines directly from ATP-rich granules. 1. Cats' adrenal glands were perfused with Locke's solution and stimulated through the splanchnic nerves or by acetylcholine.2. In response to such stimulation there appeared in the venous effluent, in addition to catecholamines, large amounts of AMP and adenosine and smaller amounts of ATP and ADP. Like the catecholamines, these substances had their origin in the chromaffin cells as was shown by their failure to appear when the splanchnic nerves were stimulated during perfusion with drugs blocking the adrenal synapses.3. During stimulation the ratio of catecholamines: ATP and metabolites in the venous effluent corresponded closely with the reported ratio of catecholamines: adenine nucleotides in the ;heavy' chromaffin granules.4. Adenine nucleotide appeared in the adrenal effluent pari passu with catecholamines within a second or two of beginning stimulation.5. It is concluded that the nucleotide-rich granules are the immediate source of catecholamines released from the stimulated adrenal chromaffin cell, and that the other two intracellular ;pools' that have been described, nucleotide-poor and ;free' cytoplasmic catecholamines, contribute little or not at all.