The effect of a thyrotrophic hormone preparation on the metabolism of radioiodine in euthyroid, hyperthyroid and acromegalic individuals.


Electroencephalography and cerebral lesions:  a clinical investigation on children.


Studies of pulmonary hypertension.  I.  Pulmonary circulatory dynamics in patients with pulmonary emphysema at rest.


Semistarvation and nutritional rehabilitation; a qualitative case study, with emphasis on behavior.


Carbon dioxide inhalation therapy of neuroses.


Dietary treatment of hypertension.  III.  The effect of protein on appetite and weight.


An alternate approach to the problem of obesity.


Hexamethonium-its effect on glomerular filtration rate, maximal tubular function and renal excretion of electrolytes.


Carbohydrate metabolism in active and static human obesity.


Psychopathology of learning and habituation.


Clinical vitamin deficiencies in patients with diabetes mellitus.


A psychopathologic concept of psychopathic personality.


Effect of B vitamin deficiencies on the leukocyte response to epinephrine and corticotropin.


The use of formula diets administered via polyethylene tube or orally for constant intake (balance) studies.


Psychosomatic aspects of dieting.

Psychosomatic aspects of dieting.


WHAT is nutrition?

WHAT is nutrition?


FOOD therapy begins with the normal diet.


Explicit analysis of topical concurrence in diagnostic interviewing.


Negro and white children's personality adjustment as revealed by a comparison of their drawings (H-T-P).


Concept formation in the schizophrenic child:  a study of ego development.


Patterns of maladjustment in children.

Patterns of maladjustment in children.


Response to electroshock therapy as evaluated by mirror drawing.


Objective studies of children's drawings of human figures.  II.  Sex, age, intelligence.


A comparison of normals and neuropsychiatric veterans on the Rosenzweig picture-frustration study.


Preschool pupil performance on the Standford-Binet and the Wechsler intelligence scale for children.


Homosexual signs in Rorschachs of early schizophrenics.


Derivation and use of the masculinity-femininity variable.


A method of weighting attitude scale items from subject responses.


Evaluation of a modification of the Thematic Apperception Test for use with physically handicapped children.


Chicago Non-verbal Examination results on an East Tennessee Negro population.


An examination of the responses of a group of young normal female and a group of female psychotic patients on the Three-Dimensional Apperception Test.


The use of Zaslow's test of concept formation on a group of subnormals.


The application of an auditory apperception test to clinical diagnosis.


Abstract behavior in elementary school children as measured by the Goldstein-Scheerer Stick Test and the Weigl-Goldstein-Scheerer Color Form Sorting Test.


Additional applications of the awol recidivist scale.


Some psychological findings in the rehabilitation of amputees.


Perceptual sensitization to sexual phenomena in the chronic physically handicapped.


The use of a graphomotor projective technique to discriminate between failure and success reactions in a level of aspiration situation.


The Goodenough Test as a measure of intelligence in child psychiatric patients.


Correlations between the Kent EGY and the Wechsler batteries.


Recognition as a criterion in the szondi test.


Patterns in delinquency.

Patterns in delinquency.


The Shipley-Hartford Vocabulary Test and pre-trauma intelligence.


The role of color in Rorschach's test:  a preliminary survey of neurotic and psychotic groups.


Reading ease and MMPI results.

Reading ease and MMPI results.


A note on sex differences on the Wechsler-Bellevue tests.


On a relation between Bender-Gestalt recall and Shipley-Hartford scores.


Back to fundamentals.

Back to fundamentals.


A theory of threat and defense.

A theory of threat and defense.


Subjects' evaluations of annoying situations after being described as well adjusted and poorly adjusted.


Stress reduction and problem-solving rigidity.


A method for assessing self and not-self attitudes during the therapeutic series.


Perceptual size constancy of known clinical groups.


College student norms for the Minnesota Multiphasic Personality Inventory.


Examiner influence on the Rorschach Inquiry.


Certain aspects of Wechsler-Bellevue scatter at low IQ levels.


The validity of Wechsler's mental deterioration index.


Deviations of Wechsler-Bellevue subtest scores from vocabulary level in university counseling-center clients.


A note on the intercard reliability of the Thematic Apperception test.


A tracer study of the transfer of F18 to teeth by topical application.


The effect of sodium fluoride upon the surface structure of powdered dental enamel.


Relationship between pH, age, and concentration of solutions of stannous fluoride and sodium fluoride in decreasing enamel solubility and affecting the uptake of fluorine.


The formation of hydroxyfluor-apatite in tooth enamel under influence of drinking water containing fluoride.


The effects of urea and salts of ammonium phosphate on salivary amylase.


The effectiveness of an ammonium ion tooth powder in the control of dental caries.


The accessibility of the organic dentinal matrix.


Dentinal protein:  amino acid composition.


The isolation of chondroitin sulfuric acid from dentin.


Experimental dental caries.  I.  The effect of orchiectomy and ovariectomy on dental caries in immature rats.


The effect of vitamin E deficiency on the rat incisor.


The reparative effect of antabuse (tetraethylthiuram disulfide) upon dental changes in vitamin E deficient rats.


Symptoms of vitamin A and E deficiencies in the incisor of the rat fed sodium sulfite.


Further studies of a differential culture technique for estimations of acidogenic bacteria in saliva.  I.  Comparison with conventional techniques and concurrent clinical examinations.


Further studies of a differential culture technique for estimations of acidogenic bacteria in saliva.  II.  Species of lactobacilli isolated from saliva and their distribution in a group of children.


Dental caries experience in primary teeth.


Dental caries experience of a group of school children at Ogden, Utah, with and without rheumatic fever.


Excitability in terms of time of the dental nervous elements.


First principles of foundational capacity in dental investing tissues.


Musical therapeutics.

Musical therapeutics.


Observations on encephalomyelitis of mice (DA strain). A new agent, DA, has been isolated from a spontaneously paralyzed mouse. Its biological properties and the pathology of lesions in experimental infection indicate close relationship with the Theiler group of encephalomyelitis viruses. Serological studies were inconclusive. The intracerebral neutralization test failed to reveal measurable antibody and other routes of inoculation were unsuitable because of low invasiveness of the agent. Repeated vaccination of females did not render their offspring resistant to homologous intracerebral challenge. The occurrence of early viremia is reported following intracerebral inoculation of the DA strain and also the known Theiler strains, 4727, FA, and GD-VII. The pathology of mice experimentally infected with DA and with known members of the Theiler group is described. Attention is called to the demyelinating lesion of the cord in mice surviving for several months and to the persistence of virus in the CNS of such animals. Another characteristic feature of the pathology was the degeneration of ventral nerve roots and in some mice of the peripheral nerves. Similar changes were not seen with strains of Theiler virus other than DA. Spheroidal bodies of undetermined significance were found in the lesions. Finally the occurrence of myositis after intracerebral inoculation, not only mice infected with the DA virus, but also with the 4727 and FA strains, is described and discussed.


Effect of sodium monofluoroacetate on the multiplication of influenza viruses, mumps virus and pneumonia virus of mice (PVM). Sodium fluoroacetate, given after virus inoculation in doses of 3 to 4 mg. per kg. in mice or 2 mg. in chick embryos, caused only a slight delay in the multiplication of the PR8 strain of influenza A virus in the mouse lung and of PR8 or the Lee strain of influenza B virus in the allantoic sac. The quantities of the compound used were sufficient to cause approximately 10 to 20 per cent mortality in mice and 100 per cent in chick embryos. The use of small virus inocula did not markedly increase the effect of sodium fluoroacetate on the multiplication of PR8 or Lee in the chick embryo and maximal titers were obtained in all cases. In contrast to the findings in the chick embryo, sodium fluoroacetate caused a definite delay in the multiplication of Lee virus in the mouse lung but did not affect the final virus titer. Sodium fluoroacetate in like amounts caused only a minimal delay in the multiplication of pneumonia virus of mice (PVM) in the mouse lung or of mumps virus in the chick embryo. With both PVM and mumps virus, maximal titers were obtained almost simultaneously in fluoroacetate and control animals. When three daily injections of the compound were given to mice infected previously with PVM, a definite diminution in the virus titer was demonstrable. However, pretreatment with three daily injections of the compound caused no alteration in the capacity of mice to support the multiplication of PVM. From the results of these experiments, it appears that the cellular metabolic processes blocked by sodium fluoroacetate are not essential for the multiplication of influenza viruses, mumps virus, or pneumonia virus of mice (PVM).


The inhibition by cortisone of the cytotoxic activity of PPD on tuberculin-hypersensitive cells in tissue culture. Using the roller tube method of tissue culture, it has been shown that cortisone and compound F inhibit the cytotoxic action of PPD upon macrophages from the splenic tissue of tuberculous guinea pigs. Pretreatment of the cells for 24 hours with hormones before exposure to PPD was found to be essential. Estrone and desoxycorticosterone glucoside had no protective activity. Certain advantages of the tissue culture method in studies of tuberculin hypersensitivity and possible mechanisms and implications of this protective action of cortisone are discussed.


The lysis of group A hemolytic streptococci by extracellular enzymes of Streptomyces albus.  I.  Production and fractionation of the lytic enzymes. The lysis of group A hemolytic streptococci by extracellular enzymes of Streptomyces albus has been studied. The most favorable material for fractionation of the lytic enzymes was obtained by surface growth on shallow layers of liquid medium containing an acid hydrolysate of casein, glucose, and salts. The results of fractionation experiments show that the potent proetolytic enzyme of Streptomyces albus is not able to lyse group A streptococci, and that the initiation of lysis is dependent upon the action of a second, non-proteolytic enzyme. The nature of the non-proteolytic enzyme has not been determined. It does not appear to be a ribonuclease or a lysozyme-like enzyme.


The lysis of group A hemolytic streptococci by extracellular enzymes of Streptomyces albus.  II.  Nature of the cellular substrate attacked by the lytic enzymes. Cell wall preparations of uniform chemical constitution have been obtained from several strains of group A streptococci. The isolated cell walls are dissolved by the same fractions of the Streptomyces albus enzymes that are effective in the lysis of intact cells, and it is likely that enzymatic lysis of group A streptococci is effected by an attack on the cell wall. The streptococcal cell wall, as prepared in this study, consists of approximately two-thirds carbohydrate and one-third protein. Small amounts of other components may be present. The carbohydrate component, which is composed primarily of N-acetyl-glucosamine and rhamnose, is the group-specific C carbohydrate. The evidence indicates that one of the streptomyces enzymes is directed toward the carbohydrate component of the cell wall.


Mutants of a bacteriophage of Bacillus megatherium. Repeated transfers in 5 per cent peptone or asparagine media of sensitive B. megatherium cultures containing C phage have made possible the isolation of lysogenic bacteria which produce different strains of phage. This is probably due to the failure of C virus to make the lysogenic adjustment while those of its variants which can succeed are selected. Four distinct plaque types have been isolated by this method and some of their reactions in mixedly infected bacteria are described. It has been shown that it is possible to isolate either C or T analogues of each of the strains, bringing the reported plaque types of B. megatherium phage to eight. No success was obtained in an attempt to isolate a wild type and a phage carrying two mutations when crosses were made of two phages each differing by one mutational step.


Strain-specific elements in influenza antigens. Rabbit antisera were prepared against ten antigenically different influenza A strains. These sera were absorbed with one or more heterologous strains and in each case all the heterologous or crossing antibody was removed; the anti-bodies remaining after this treatment were specific for the immunizing strain or group of strains. On the basis of reactions with absorbed sera, the strains fell into seven groups. Absorbed specific antisera of these groups were used to test the HI titer against a large number of influenza A viruses. Most of the strains were inhibited by a single serum, a few were inhibited by none of the sera, and only one strain was inhibited by two antisera. The grouping of strains by this method was less equivocal than classifications based on previous tests. When more fully developed, this technique promises to be of interest and assistance in the study of influenza, especially from the epidemiological and prophylactic standpoints.


Studies on the generalized Shwartzman reaction:  I.  General observations concerning the phenomenon. Certain factors involved in the production of the generalized Shwartzman reaction with meningococcal toxin in rabbits were investigated. The optimal amounts of toxin for the preparing and provoking injections, and the optimal time interval between injections were determined. Under suitable conditions of dosage and timing, bilateral cortical necrosis of the kidneys was produced in a high proportion of animals. When excessive amounts of toxin were used for preparation the incidence of the reaction was reduced. Animals undergoing the generalized Shwartzman reaction became severely prostrated within several hours after the provoking injection of toxin. The renal lesion became fully developed within 24 hours, and its occurrence was associated with a rise of the blood non-protein nitrogen. Edema and petechial hemorrhages in the ears were observed in rabbits with advanced renal lesions. The earliest change in the kidneys in the generalized Shwartzman reaction was the appearance of homogeneous, eosinophilic material, resembling fibrinoid, within the lumen of the glomerular capillaries. Occlusion of the capillaries by this material was regarded as the cause of subsequent tubular necrosis in the renal cortex. The material appeared to be derived from the blood, rather than from the capillary walls. Cortisone enhanced the lethal effect of a single, large dose of meningococcal toxin, as well as causing bilateral renal cortical necrosis. The generalized Shwartzman reaction produced by two injections of toxin was aggravated by cortisone and ACTH. Profound polymorphonuclear leukopenia was produced by both the preparing and provoking injections of toxin. When leukopenia was produced before the preparing injection of toxin, by treatment with nitrogen mustard, the generalized Shwartzman reaction was inhibited. During the intervals before and after leukopenia, and when leukopenia was prevented by shielding the femoral bone marrow from the action of nitrogen mustard, no inhibition of the generalized Shwartzman phenomenon was demonstrable. Various colloidal and particulate materials, which are capable of provoking the local skin Shwartzman reaction when injected intravenously, failed to provoke the generalized Shwartzman reaction. A working hypothesis was set up to account for certain events in the generalized Shwartzman reaction.


Studies on the generalized Shwartzman reaction.  II.  The production of bilateral cortical necrosis of the kidneys by a single injection of bacterial toxin in rabbits previously treated with thorotrast or trypan blue. Intravenous injection of thorotrast or trypan blue rendered rabbits susceptible to the production of bilateral cortical necrosis of the kidneys by a single intravenous injection of small amounts of meningococcal or Serratia marcescens toxin. This reaction was not produced when thorotrast or trypan blue were injected after toxin had been given. A single intradermal injection of toxin produced hemorrhagic skin lesions resembling the local Shwartzman reaction in rabbits given thorotrast 6 hours previously. These animals also developed bilateral cortical necrosis of the kidneys. When the order of injection was reversed, and thorotrast given after toxin, neither skin nor kidney lesions occurred. The skin and kidney lesions in thorotrast-treated rabbits were, like the local and generalized Shwartzman reactions, completely prevented by treatment with nitrogen mustard, in doses sufficient to produce polymorphonuclear leukopenia. The significance of these reactions, and their relationship to the previously described response to toxin in cortisone-treated rabbits, are discussed.


Studies on the mechanism of experimental proteinuria. When renin is administered intramuscularly to the rat, massive proteinuria occurs without a significant elevation of mean arterial blood pressure. The intravenous administration of renin to normal rats results in a great increase in urinary protein excretion. This response to renin is abolished by bilateral adrenalectomy. While the adrenalectomized rat fails to respond to intravenous renin with increased proteinuria, it does exhibit a normal elevation in mean arterial blood pressure. It is concluded that in the rat, the proteinuric property of renin is not related to the ability of this compound to elevate arterial blood pressure. The passage of plasma proteins has been followed through the kidney and into the urine by attaching them to the dye T-1824 (Evan's blue). The intra-peritoneal injection of renin causes a massive, transient proteinuria in the rat. From a study of frozen sections of the kidney of rats whose plasma proteins are labelled with T-1824, it is concluded that the preponderant basis for renin proteinuria is an increase in glomerular permeability.


Fate of mumps virus in the embryonated egg as determined by specific staining with fluorescein-labelled immune serum. Specific staining with fluorescein-labelled immune serum was used to study the progress of mumps virus infection in a series of embryos harvested at daily intervals. The results were compared with the hemagglutinin and infectivity titers of the corresponding extraembryonic fluids. The evidence obtained indicated that the multiplication of the virus was restricted to those cells which came into surface contact with infected fluid. Following intraamniotic inoculation into 8 day old embryos, these included the cells lining the amniotic membrane and the epidermal and pharyngeal epithelium. Depending apparently on the extent of the contamination of the allantoic cavity and of the extraembryonic celom in the course of inoculation, varying amounts of virus were also present in the cells lining the chorioallantoic membrane and occasionally in the peritoneum. During the later stages of the infection, staining which was principally if not entirely extracellular was seen in the gastrointestinal tract, and, after the dissolution of the tracheal plug, in the respiratory tract. The virus was first detected 1 to 2 days after inoculation as brightly fluorescent intracytoplasmic granules of varying size. As these granules increased in size and number they gradually filled the cytoplasm of the outer one or two layers of cells lining the adjoining cavity. The extent and brightness of the staining reached a peak 4 to 6 days after inoculation and thereafter decreased progressively. The staining of the tissues closely paralleled the rise and fall in the infectivity of the extraembryonic fluids. The development of hemagglutinins, on the other hand, provided a less sensitive measure of virus multiplication than did the staining. No differences were detected in the mortality of the infected and control series of embryos nor was there evidence of any pathological changes. In contrast to the 8 day old embryos, no virus multiplication was detected following the inoculation of 16 day old embryos.


Studies on the virulence of tubercle bacilli; variations in virulence effected by tween 80 and thiosemicarbazone. Tubercle bacilli were grown in the presence of different concentrations of tween 80, ranging from 0.05 to 2.1 per cent. Equal numbers of viable bacteria from these cultures were compared in infection experiments in the mouse. The average survival time of the mice was used as a criterion for the virulence of the bacilli. High tween concentrations in the culture medium caused a reduction of the bacterial virulence. The reduction was slight in bacterial suspensions from cultures with tween 80 ranging from 0.05 to 1.0 per cent, but considerable in cultures with 2.1 per cent tween. Bacteria grown in the presence of 2.1 per cent tween gave rise to the same number of colonies, in vitro, as bacteria grown in ordinary media. Their oxygen uptake was increased as compared with that of bacilli grown in media containing less tween. Virulent bacteria grown in the presence of high amounts of tween 80 decolorized methylene blue in a test in which organisms from the same virulent strain but cultured without tween, or with only small proportions of the detergent in the medium, did not reduce the dye. A positive methylene blue test is typical of non-virulent tubercle bacilli and of saprophytic mycobacteria. Essentially the same changes occurred when virulent tubercle bacilli were grown in the presence of 0.5 microg./ml. of para-formacetanilide thiosemicarbazone (TBI). This small amount of the substance was not sufficient to prevent the growth of bacteria, or to reduce the number of viable cells in a culture, but it reduced the virulence of the bacteria considerably and rendered them capable of decolorizing methylene blue. Cord factor, a lipid constituent of virulent bacteria which is toxic for mice, was shown to be present in filtrates from cultures of virulent bacteria when the media contained 2 per cent tween 80, but no such material could be recovered from culture filtrates containing the usual 0.05 per cent tween. On the other hand, no toxic material could be extracted from bacteria grown in the presence of 0.5 microg./ml. TBI.


Induction of streptomycin resistance in sensitive Hemophilus influenzae by extracts containing desoxyribonucleic acid from resistant Hemophilus influenzae. Resistance to streptomycin, of a degree exceeding 1000 microg. per ml., has been induced in sensitive strains of Hemophilus influenzae by exposure for 10 minutes to desoxyribonucleic acid-containing extracts isolated from a strain of type b Hemophilus influenzae which had emerged resistant to 1000 microg. of streptomycin per ml. DNA is essential for the process which brings out this change; the reaction can be prevented by destruction of the DNA with crystalline desoxyribonuclease.The resistant trait which is created in this way is heritable. The nature of the process which induces resistance is similar in all respects to the reaction which induces heritable changes in type specificity of H. influenzae. These results offer another example of the gene-like action of highly specific DNA's. The pattern of resistance brought out in a bacterial population exposed to the DNA-containing, resistance-inducing extract, is similar to that which occurs when emergence of resistance of H. influenzae to streptomycin follows the selection by streptomycin of spontaneously occurring resistant mutants. The change in a bacterial cell from average susceptibility to streptomycin to resistance to 1000 microg. of streptomycin per ml. can occur in a single step.


Experimental erythroblastosis fetalis in rabbits.  I.  Characterization of a pair of allelic blood group factors and their specific immune isoantibodies. A pair of blood group factors, designated G and g, was identified in rabbits by serological means. These factors were found to be alleles, and one or the other or both of them were regularly present in the red cells of every one of a large number of mongrel and inbred rabbits. The factors were not demonstrable in other tissue cells or in the body fluids. They were capable of stimulating the formation of specific immune isoantibodies when repeatedly injected into appropriate rabbits. In most instance both agglutinating and coating antibodies to the G or g factors were present in a given antiserum. The coating antibodies-which did not act as true blocking antibodies-were detected by means of an antiglobulin test (Coombs test), or by means of specific cells modified by the action of trypsin. The antibodies were heat-stable and were active over a wide temperature range; and under suitable conditions, they proved to be potent hemolysins and also capable of fixing complement. The characteristics of the antigens and antibodies of the rabbit G-g system bear a striking resemblance to those of the Rh-Hr system of man.


Experimental erythroblastosis fetalis in rabbits.  II.  The passage of blood group antigens and their specific isoantibodies across the placenta. Female rabbits became immunized during pregnancy to the rabbit blood group factors G or g in five out of ten instances in which the red cells of the fetuses carried one of the factors absent in the mother. Antibodies so produced were of low titer and disappeared in all cases within 6 weeks after the birth of the litter. Repeated pregnancies did not result in additive increases in titer. Antibodies to the G-g factors, whether produced by the injection of red cells or by pregnancy, crossed the placenta readily from mother to fetus and were found at birth (and prior to nursing) associated with the red cells and in the serum of the fetuses. The rabbit placenta appeared to be equally permeable to the agglutinating and coating antibodies. The implications of these findings and their relation to the pathogenesis of erythroblastosis fetalis are briefly touched upon.


Tuberculosis induced by droplet nuclei infection; initial homogeneous response of small mammals (rats, mice, guinea pigs, and hamsters) to human and to bovine bacilli, and the rate and pattern of tubercle development. Separate groups of rats, mice, hamsters, and guinea pigs were caused to inhale virulent tubercle bacilli, of human or bovine strains, as single cells in fine droplet nuclei. Members of each of these eight host-parasite combinations were killed for study at stated intervals after infection. For approximately 3 weeks after the bacilli were deposited in the lungs the progress of the infection, and the reaction of all species to it, followed a highly uniform developmental pattern. During the 4th week the rate and pattern of tubercle formation became distinctive for the species of host and the strain of parasite, but within any host-parasite combination this rate and pattern continued uniform for a time. The duration of this period of homogeneous response after the 4th week varied with the host-parasite combination, ranging from less than 5 weeks to more than 12 weeks after the induction of infection. It is concluded that the highly uniform initial response is evidence that these animals do not differ in their inherent resistance to inhaled infection by the bacilli of human or bovine tuberculosis. Instead, they differ widely in their capacity to acquire resistance to these organisms, as shown by the variation in the later stages of the disease.


Studies on entry and egress of poliomyelitic infection.  V.  Entry after simple feeding:  with notes on viremia. Poliomyelitis virus, when ingested by cynomolgus monkeys in their regular food, infected peripheral ganglia (nodose) as early as the 3rd day, as shown by recovery of the virus. Conditions on the 2nd and 4th days were not investigated, but on the 5th and 6th days, virus was recovered from the Gasserian and nodose ganglia, and from the superior cervical sympathetic and celiac ganglia. The findings indicate that the method of oropharyngeal swabbing used in a study already reported and the method of simple feeding used in the present study produce comparable results. Viremia, noted in the present study, was contemporaneous with virus recoveries from the ganglia. Reasons are presented why the peripheral ganglia are the most probable source of viremia since these are the only known site of early lesions (which are typical and appear as early as the 2nd day after oral infection) and no lesions are known to occur in extraneural tissues. Invasion of the CNS occurred in 3 animals out of 11; one on the 5th and 2 on the 6th day. Whether this was due to viremia or to centripetal extension along axonal channels is not clear.


The protection of intracellular brucella against therapeutic agents and the bactericidal action of serum. A method for the in vitro study of intracellular brucella has been described. Exudative leukocytes containing intracellular brucella have been maintained in vitro in a synthetic tissue culture medium or in human or animal serum. Intracellular brucella are protected in vitro against the lethal action of therapeutic agents or the bactericidal action of serum. This protection of intracellular brucella is dependent upon the presence of an intact, viable host cell. None of the currently available therapeutic agents, whether used alone or in combinations, were capable of killing all intracellular brucella in vitro in 24 hours. A remarkable protection of intracellular brucella against streptomycin has been demonstrated. The most effective reduction in the number of viable intracellular brucella was accomplished by exposure of the host cells to streptomycin plus aureomycin, terramycin, or chloramphenicol. The available evidence suggests that the ability of brucella to localize and remain viable within the cells of an infected host is an important biologic factor in establishing and perpetuating brucella infections, despite therapeutic measures or the operation of the host's humoral defense mechanisms. Reduction of neotetrazolium by leukocytes and brucella in vitro provides a method for assessing the metabolic status of the host cell, but does not discriminate with any degree of certainty a viable from a non-viable intracellular organism.


The intracellular localization of poliomyelitis virus. A study was made of the intracellular localization of Type 2 poliomyelitis virus, using the technique of Mirsky and Pollister (23) for cellular fractionation. After isotonic saline homogenization of central nervous system tissue from infected mice, and subsequent centrifugation of the suspension, the virus present in the supernatant fluid was held to be of cytoplasmic origin. Upon serial washings of the sediment with physiological saline, the resulting supernates contained progressively less virus until by the seventh washing, virtually none was present. At this point extraction of the washed sediment with molar NaCl, which lyses the nuclei, yielded substantial amounts of virus, and this was assumed to be from nuclear sources. The possibility has not been excluded however that the "nuclear" sediment was contaminated by cytoplasmic particles too large to remain in the supernate. Experiments on the increase of virus during the incubation and acute stages of infection have revealed that it was first detectable in the "cytoplasmic" fraction and subsequently in the "nuclear" fraction. Virus in the "nuclear" fraction from paralyzed mice sometimes reached titers almost as high as those found in the "cytoplasm." Adsorption experiments indicated that the "nuclear" fraction of CNS tissue from normal, uninoculated mice did not adsorb added Type 2 poliomyelitis virus, nor did such fractions adsorb virus procured from the "cytoplasm" or "nuclei" of infected cells. Although individual mice varied in their response after virus injection, the "cytoplasmic" fraction of paralytic mice was found to contain virus regularly, whereas little more than half of the non-paralytic mice yielded it. When virus was present in the "cytoplasm," it could be found in the "nuclear" fraction of paralytic mice with much greater regularity than in that of non-paralytic mice. A comparison between the lines of the MEF1 strain of poliomyelitis virus, "adapted" and "non-adapted" to newborn mice, and the Lansing strain, revealed no differences in their intracellular increase. In both infant and adult mice, the chief difference in the findings with non-paralyzed and paralyzed mice lay in the greater concentration of virus in the "nuclear" fractions of the latter group.


The effect of x-radiation on the oxygen uptake of embryonate eggs. Groups of 10 day old embryonate eggs were given 250, 500, 750, and 1000 r of x-radiation, and oxygen consumption determinations were made subsequently at various intervals during a period of 90 hours. In general, the effect of radiation was moderately stimulatory, but after 90 hours the respiratory rates of the 250 and 1000 r groups were well below those of the control groups. The most striking effect, noted in 3 separate experiments, was the occurrence of a "3 step staircase" type of oxygen consumption curve in all irradiated groups, the levelling off periods apparently occurring between the 4th and 14th, the 24th and 36th, and the 42nd and 66th hours after exposure. These curves were in striking contrast to those shown by groups of non-irradiated eggs, which were practically of the straight line type.


The mechanism responsible for the hypercholesteremia induced by triton WR-1339. Injection of triton WR-1339 into rats leads to a rapid increase in the cholesterol, cholate, and various lipid fractions of their blood. The increase in cholesterol is confined to the blood itself. The cholesteremic effect of triton was not dependent upon a prior accumulation of cholate in plasma. The liver was found to be the source of the excess cholesterol but the rate of cholesterol synthesis, the excretion of cholate, and the cholesterol content of the liver were not changed by injection of triton. The cholesteremic effect of triton is not due to alteration in the intestinal excretion of cholesterol. Transfer of blood between "tritonized" and normal rats leads to a disappearance of the hypercholesteremia in the former and its appearance in the latter animals. The plasma proteins of rats injected with triton are markedly changed qualitatively. Heparin was found to inhibit the hypercholesteremic effect of triton. The hypercholesteremia following triton injection appears to be due to a fundamental physicochemical change in the plasma proteins produced by injection of this detergent.


The effect of x-radiation on the multiplication of Rickettsia mooseri in embryonate eggs. The multiplication of Rickettsia mooseri in fertile eggs is speeded up and quantitatively increased by single dose x-radiation given either 24 hours before or 48 hours after inoculation. This effect is noted at all dosage levels studied, ranging from 100 to 1500 r. The rickettsiostatic effects of high incubation temperature (40 degrees C.) and of streptomycin are neutralized by radiation, but the rickettsiostatic actions of PABA, penicillin, and aureomycin are not altered. Possible mechanisms of action and implications of the observed effects are discussed.


The effect of x-radiation on the multiplication of influenza A virus in embryonate eggs. Groups of embryonate eggs were given 250, 500, 750, and 1000 r of x-radiation. 18 hours later, these 4 groups, together with a control group, were injected intra-allantoically with influenza A virus. Radiation at all dosage levels caused significant changes in the infectivity titre curves during the next 90 hours. The most striking alterations were the prolonged incubation period and the uniformly low infectivity titre in the group of eggs given 750 r.


The localization of homolgous plasma proteins in the tissues of young human beings as demonstrated with fluorescent antibodies. Employing fluorescent antibodies for the detection of homologous plasma proteins in tissue sections, the distribution of plasma albumin, gamma-globulin, beta-lipoprotein, beta(1)-metal-combining globulin, and fibrinogen has been studied in the tissues of infants and children. Plasma albumin, gamma-globulin, and beta(1)-metal-combining globulin were found in many cells and particularly cell nuclei, connective tissues and interstitial spaces, lymphatics, and blood vessels. beta-Lipoprotein was found mostly in the nuclei of all cell types while fibrinogen was restricted largely to the lymphatic and vascular channels, connective tissues and the interstitial spaces. The widespread distribution of these plasma proteins in cells and connective tissues indicates the magnitude of the extravascular plasma protein pool which is in equilibrium with circulating plasma. Unfortunately, these results do not permit accurate localization of the sites of production of these plasma proteins, but do give some idea of their intimate relationship to the tissues.


Structural changes produced in Brown-Pearce carcinoma cells by means of a specific antibody and complement. Structural changes were regularly observed in Brown-Pearce rabbit carcinoma cells that had been brought into contact, in the presence of complement, with rabbit serum containing an antibody that reacts specifically with a distinctive sedimentable constituent of the carcinoma cells. The cellular changes appeared rapidly and were accompanied by an equally rapid loss of viability. The structural changes, as disclosed by histological and cytological methods, including phase contrast microscopy, were described in detail and illustrated. Essentially they were characterized by the entrance of considerable quantities of fluid into the cell, together with swelling and disorganization of its cytoplasm, vesiculation and rupture of the bulk of its cytoplasmic particles, and rapid and virtually complete loss of cytoplasmic basophilism. The plasma membranes, though stretched thin about the swollen cytoplasm of the altered cells, and otherwise changed also, remained unruptured during many hours' observation. The nuclei of the altered cells, however, remained relatively un-changed, their membranes persisting unruptured and their sap remaining wholly transparent and undiminished in amount for long periods, while within it the chromatin retained its staining properties, though sometimes becoming clumped and marginated; by contrast, the nucleoli of the treated cells promptly shrank and lost much of their affinity for the basic dyes. The experimental conditions under which the structural changes became manifest were given in detail, together with an analysis of the findings and brief mention of certain of their implications for cytology and immunology.


The development of Leishmania donovani in vitro at 37 degree C; effects of the kind of serum. Suspensions of leishmanias from the spleen of hamsters infected with Leishmania donovani were placed in culture flasks and incubated at 37 degrees C. In a medium of human erythrocyte extract and human serum there appeared within a day or two aflagellate forms resembling leishmanias but larger, as well as other aflagellate forms more nearly resembling rounded leptomonads. These intermediate forms multiplied during the first 4 days of culture. They then slowly died off, despite frequent renewal of the culture medium. Sometimes a small proportion of motile, typical leptomonads also appeared in such cultures. Leptomonads from cultures maintained at 28 degrees C., when placed in the human red cell extract-human serum medium and incubated at 37 degrees C., survived at least 4 days. For both types of effect, human serum could be replaced by normal hamster serum but not by rabbit serum. Nicotinamide, added to the human red cell extract-human serum medium at a concentration of 400 mg. per 100 ml., completely prevented the development of intermediate forms from leishmanias and brought about the rapid death of leptomonads at 37 degrees C.


Antituberculous immunity induced in mice by vaccination with living cultures of attenuated tubercle bacilli. The immunity induced in mice by vaccination with living attenuated cultures of tubercle bacilli was measured by two criteria. (a) Increase in survival time of the vaccinated animals after infection with a dose of virulent bacilli sufficient to kill all the unvaccinated controls within 10 to 20 days. (b) Difference in the number of living bacilli recovered from the spleen and lungs of vaccinated and normal animals infected with a small dose of virulent bacilli. The level of immunity induced was found to depend upon the extent of multiplication in vivo of the bacilli used for vaccination. This in turn was conditioned by the degree of attenuation characteristic of the bacterial strain used in the preparation of the vaccine, the amount of vaccine injected, the route of vaccination, and the time interval between vaccination and challenge infection. It was possible to prevent or retard the development of immunity by treating the mice in course of immunization with a drug, isoniazid, capable of interrupting the multiplication in vivo of the bacilli used as vaccine. Although immunity regularly developed and lasted for many weeks when the proper conditions of vaccination were used, the immune response was never sufficient to protect the animals against ultimate death from infection with virulent tubercle bacilli. The prolongation of life in the vaccinated mice was not consequent on a direct bactericidal effect but rather on a retarded or interrupted multiplication of the virulent bacilli in vivo. The quantitative bacteriological techniques used in the present study would appear to be of value for the analysis of certain problems of immunity, and for the appraisal of vaccines and techniques of vaccination.


Multiplication and survival of tubercle bacilli in the organs of mice. Cultures of tubercle bacilli (typical bovine and human strains) known to differ in the severity of the lesions they induce in experimental animals, were injected in various doses into the cerebrum, peritoneal cavity, or blood stream of mice. Quantitative determinations of the numbers of living bacilli present in the tissues at different intervals of time after infection led to the following classification of the cultures tested:- (a) Certain well known variant forms of tubercle bacilli were found to be unable to multiply in vivo, although they could survive for many weeks in the tissues of mice. These organisms proved to be truly avirulent. (b) Other variant forms underwent multiplication in vivo, even when extremely small infective doses were used, but could not give rise to progressive disease. It is proposed to designate these strains, which produce only abortive infections, as "attenuated." Different levels of attenuation could be detected. The maximum numbers of living bacilli that were recovered from the tissues corresponded directly to the severity and duration of the abortive lesions that could be produced by the strain in guinea pigs or in mice and were characteristic for each strain tested. The two BCG substrains tested were found to differ markedly in their level of attenuation. (c) The cultures virulent for guinea pigs were also capable of establishing a progressive infection in mice even when small infective doses were used. In the case of the attenuated and virulent strains, the population of living bacilli present in the lungs was at first much lower than that in the spleen, but it continued to increase in the former organs throughout the period of observation. This was notably true in the case of the virulent cultures. In contrast, the numbers of living bacilli in the spleen rapidly reached a maximum in the case of all cultures and then decreased progressively. For a given infective dose, and a given interval of time after inoculation, the maximum levels of living bacterial population attained in the spleen and in the lungs proved to be a direct expression of the virulence of the strain.


Antituberculous immunity in mice vaccinated with killed tubercle bacilli. The resistance of white mice to tuberculous infection could be increased by preliminary vaccination with small amounts of tubercle bacilli killed by contact with 2 per cent phenol. Vaccine prepared from a variant strain of human tubercle bacilli unable to multiply in vivo (H37Ra) proved as active as vaccines prepared from either virulent or attenuated strains. The immunity induced by phenol-killed bacilli persisted for several weeks. Under the conditions of the experiments, however, it was never able to bring about the death of the virulent bacilli used for the challenge infection, even when the infective inoculum was very small. Its protective effect could be detected (a) by the increased survival time of mice infected with a very large dose of virulent bacilli, and particularly (b) by the lower numbers of bacilli present in the organs of mice sacrificed at various periods of time after injection of sublethal infective doses. Under the proper conditions of vaccination the immunity produced in mice by phenol-killed cells of avirulent bacilli was of the same order as that produced by BCG. The protective antigen proved to be susceptible to heat, particularly at acid reactions. It retained its activity when the bacilli were disintegrated and rendered non-acid-fast by grinding with concentrated phenol. It remained in the insoluble cellular debris when the bacilli were extracted with 88 per cent phenol. Reasons are presented to support the view that the antigenic components present in the tubercle bacilli (avirulent as well as virulent) killed with phenol play a significant part in several manifestations of increased resistance to tuberculosis.


Multiplication of tubercle bacilli within mononuclear phagocytes in tissue cultures derived from normal animals and animals vaccinated with BCG. When monocytes derived from normal guinea pigs or rabbits were infected with tubercle bacilli and cultivated in vitro, the bacilli multiplied abundantly within the cytoplasm of these cells. By contrast, intracellular multiplication of the bacilli was retarded or completely inhibited within the monocytes of rabbits or guinea pigs vaccinated with BCG. This inhibition of growth was observed with both virulent or attenuated strains of tubercle bacilli. Under the conditions used in the present study, the ability of monocytes to inhibit bacillary proliferation was the same whether serum from a normal animal or from vaccinated animals was used in the tissue culture medium. Moreover, the serum of vaccinated animals did not inhibit multiplication of tubercle bacilli within monocytes derived from a normal animal. The ability of guinea pig monocytes to interfere with intracellular bacillary proliferation was first perceptible 8 days after vaccination.


On the nature of bacteremia in experimental pneumococcal pneumonia in the dog.  I.  Relationship of natural pneumococcidal-promoting activity of the serum to blood invasion. IN A STUDY OF THE RELATIONSHIP OF NATURAL ANTIPNEUMOCOCCAL IMMUNE SUBSTANCES TO THE INCIDENCE AND COURSE OF BACTEREMIA IN DOGS WITH EXPERIMENTAL PNEUMOCOCCUS PNEUMONIA THE FOLLOWING FINDINGS CAME TO LIGHT: (1) In non-bacteremic animals, natural immune substances, as measured by the pneumococcidal-promoting action of the serum, continue to be present in relatively undiminished concentration throughout the course of the infection. (2) With the advent of bacteremia these immune properties of the blood tend to decrease or disappear, depending on the degree of bacteremia and the length of the disease course, but in certain instances they persist despite the presence of large numbers of circulating pneumococci. (3) Disappearance of natural immune substances from the blood during bacteremia is followed by their reappearance upon cessation of the bacteremia. (4) Bacteremic blood containing antipneumococcal immune substances and a sufficient quantity of leucocytes is capable of destroying in vitro relatively large numbers of pneumococci and will often sterilize itself. (5) The sequence of bacteremia first, then diminution and disappearance of humoral immunity excludes this antipneumococcal action of the blood as being the principal inhibitor of blood invasion. These observations have been interpreted as indicating that the bacteremic state consists of a constant escape of pneumococci from the pulmonary lesion and an attempt on the part of the body to compensate for the depletion of circulating immune substances resulting from their progressive immobilization by the pneumococci and their products. Thus, the loss or retention of humoral immune substances in the presence of bacteremia would appear to depend on the rate at which the body can provide new supplies of antibodies and on the number of pneumococci being discharged into the circulation. While the pneumococcidal action of the blood may not be sufficient to prevent the occurrence of bacteremia our study provides ample evidence that it exerts a potent restraining effect on the increase in numbers of pneumococci in the circulation.


A comparative histologic and immunologic study in rabbits of induced hypersensitivity of the serum sickness type. A histologic and quantitative immunologic study was made on a large group of rabbits injected intravenously with a single dose of crystallized bovine albumin and bled and sacrificed at various intervals after injection. Both cardiovascular and renal lesions were encountered in high incidence. The various stages in the development of these lesions were observed from their first appearance until their complete regression. The cardiac, vascular, and renal alterations were morphologically similar to those of rheumatic fever, polyarteritis nodosa, and acute glomerulonephritis, respectively, in man. In addition to those tissue changes ordinarily attributed to hypersensitivity, peculiar granulomatous lesions consisting of epithelioid and foreign body giant cells were encountered in the follicles of the spleen and in the lymph nodes of a large proportion of the animals receiving the antigen. Similar granulomas in the spleen and lymph nodes as well as in other tissues have been described in polyarteritis nodosa. The present experimental demonstration that an epithelioid and giant cell reaction may result from hypersensitivity provides evidence for the allergic origin of those cases of polyarteritis nodosa of unknown etiology containing this type of lesion. In the present study the time of development of the cardiovascular, renal, and granulomatous lesions was determined in relation to the blood clearance of antigen and the time of appearance of circulating antibody. All the tissue lesions developed during the "immune" phase of antigen elimination and regressed after the antigen had been completely eliminated and free antibody had appeared in the circulation. These temporal relationships indicate that the tissue lesions which occur after the intravenous administration of foreign protein are the result of antigen-antibody combination.


Antigen retention in the rabbit.

Antigen retention in the rabbit. Normal and irradiated rabbits were given small doses of I(131) bovine gamma globulin (BGG) and the rates of its loss from their blood were determined. The figures agreed with those of previous reports. 3 to 4 months later, both groups were reinjected with antigen. The control group gave an accelerated (anamnestic) rate of loss, indicative of the immune state. The irradiated group gave a response similar to that given by the control group on primary injection. Rabbits recover from 500 roentgens of x-ray in less than 2 months. Hence, rabbits given BGG soon after irradiation should become immune to this antigen in 2 months if they retain it. Since rabbits reinjected with BGG 3 to 4 months after irradiation did not give an accelerated response, the BGG must not have been retained over the period of time necessary for recovery; i.e., less than 2 months. As the rate of antigen loss is greater in normal than in irradiated animals, normal rabbits will have lost BGG as active antigen prior to the irradiated animals. The amounts of antigen used (0.25 to 0.4 mg./kg.) more nearly approximate the amounts present during disease than have the amounts used in previous studies of antigen retention. The hypothesis that protein antigens are lost as fast or faster than homologous proteins is discussed and the conclusion reached that this is a valid concept.


On the nature of bacteremia in experimental pneumococcal pneumonia in the dog.  II.  Disappearance of pneumococci from the circulation in relation to the bactericidal action of the blood in vitro. With the purpose of ascertaining the influence exerted by the pneumococcidal activity of the blood on the course of bacteremia occurring in experimental canine pneumococcal pneumonia, a study was made of the rates at which intravenously injected pneumococci disappeared from the circulation and the shed blood of diseased dogs. Preliminary studies on normal animals showed that blood containing hundreds of thousands of pneumococci per cc. immediately after injection usually became sterile or nearly so within an hour's time. Simultaneous observations carried out on the blood in vitro showed an analogous rapid disappearance of the microorganisms, although the effect was not quite as marked. Similar tests on non-bacteremic dogs with pneumonia revealed essentially the same ability of the body to dispose of large numbers of circulating pneumococci. The shed blood likewise exhibited marked bactericidal power. The occurrence of bacteremia during pneumonia did not retard greatly the rate at which injected pneumococci disappeared from the circulation, as compared with the non-bacteremic state. After several hours the numbers of circulating microorganisms were approximately the same as prior to the intravenous injection. Blood in vitro often cleared as fully as it did in vivo over the same length of time. Studies on the role played by humoral immune substances in the bactericidal action of the blood showed that while their presence was necessary for maximum killing power, and that bacteremic blood lacking humoral immune properties was rarely capable of self-sterilization in vitro, nevertheless such blood often retained considerable bactericidal potency as shown by its ability to reduce materially the numbers of pneumococci added to it. This phenomenon is discussed. The marked pneumococcidal capacity of the blood exhibited by dogs with experimental pneumococcal pneumonia and its persistence during bacteremia suggest that this constitutes the principal mechanism for limiting the degree of blood invasion. The similarity of the findings in canine and human pneumococcal lobar pneumonia is pointed out.


Production, extraction and purification of haemagglutinin of Haemophilus pertussis.


A comparison of methods for removing trace metals from microbiological media.


Factors affecting oxidation of acetate by Bacterium coli.


Complete and reduced life cycles in Rhizobium.


A microbiological assay of inositol:  its development and statistical analysis.


Conditions affecting the growth of Bacterium coli on bile salts media; enumeration of this organism in polluted waters.


On the transference of bacterial antibodies from the hen to the chick.


A phase-contrast study of reproduction in mycelial strains of avian tubercle bacilli.


The stability of antibiotics in soils.

The stability of antibiotics in soils.


Susceptibility of pleuropneumonia-like organisms from human genital tract to the action of soaps.


Variation in the antigenic composition of staphylococcal coagulase.


Adaptation of 'B' virus to the Swiss albino mouse.


The stability of some bacterial enzymes toward heat and chemical bactericides.


Some factors affecting the destruction of Bacterium coli by shaking with glass beads.


Effect of specific polysaccharides from the host bacteria and of ribonuclease on the multiplication of rhizobium phages.


Investigations on the microbiology of cellulose utilization in domestic rabbits.


The incidence of airborne Cladosporium spores in the London region.


Diphtheria bacteriophages and their relation to the development of bacterial variants.


The inhibition of growth of avirulent strains of Mycobacterium tuberculosis by a surface-active polyoxyethylene ether.


The phage typing of Shigella sonnei, and the limits of type stability.


In vitro and in vivo properties of culture filtrates of Bacillus subtilis with high gelatinase activity.


The uptake of cobalt by Proteus vulgaris.


An analytical study of in vivo survival of limited populations of animal red blood cells tagged with radio-iron. Animal red blood cell in vivo survival curves, obtained by the radioiron tagging of populations of approximately the same age followed by the administration of non-radioactive iron to suppress radioiron reutilization, have been subjected to mathematical analysis on the basis of the three following assumptions:- (A) Red blood cells disappear from the circulation as the result of senescence: there is an average life span around which the life spans of individual cells are distributed in the usual way. (B) Red blood cells may be removed from the circulation by a process of random destruction which continuously removes a constant fraction of the cells present at any moment irrespective of age or other characteristics. (C) Under the conditions of the experiments described, a fraction of the radioiron, constant for each animal, is reutilized in new red cell formation when released by red cell destruction. This mathematical analysis indicates the following average life spans with the respective standard errors of the mean: dog 107 days +/- 1.14; rabbit 67.6 days +/- 1.94; cat 68.4 +/- 1.50. The mathematical treatment presented has permitted a consideration of the theoretical variation of red cell life spans which was found in these experiments to be relatively small for all three species studied. In the rabbit and cat 2.5 per cent of tagged populations of red cells of the same age would theoretically have disappeared by senescence 17 days before the average life span was reached. The variation of red cell life in the dog was slightly less. Animals of the three species studied, in spite of apparently normal health, exhibited varying degrees of random destruction of both autogenous and transfused fresh normal homologous red cells. As yet, we have no explanation for this random loss of cells occurring in apparently healthy normal animals. The method of mathematical analysis presented is applicable to animal red cell survival studies employing radioiron in which differing rates of random destruction are operating in the removal of red cells.


Protection of bacteriophage against x-rays by high concentrations of a neutral salt. Protection of bacteriophage T1 against x-rays was tested in the presence of concentrations of (NH(4))(2)SO(4) ranging from 10(-6)M to saturation (4.26 M). Survival of T1 in concentrations of 10(-6) to 10(-3)M after irradiation did not differ significantly from survival in distilled water after irradiation. From 10(-3)M to 10(-1)M there was a steep rise in survival, with a leveling off as the concentration approached saturation, giving over-all a 2,000-fold increase in survival. The mechanism of salting out protection in these experiments is apparently due chiefly to dehydration, which protects the virus particles against the indirect effects of x-irradiation. Postirradiation effects, tested by the inactivation of phage added to irradiated media, approach in magnitude the effects obtained by irradiation of the phage particles themselves in the various solutions. Filter paper adsorption analyses indicate a close correlation between concentrations of (NH(4))(2)SO(4), ability of the filter paper to adsorb phage, and protection against x-rays, both during and after irradiation.


Nutrition of the European corn borer, Pyrausta nubilalis (HBN).  III.  An unidentified dietary factor required for larval growth. 1. The European corn borer larva has been shown to require a dietary source of one or more unidentified factors contained in corn leaves, grass juice concentrate, and certain other plant materials. The required factor or factors has been termed the corn leaf factor. 2. The corn leaf factor does not appear to be identical with any of the known B vitamins, or with ascorbic acid, sodium nucleate, citrovorum factor, or carnitine. 3. The corn leaf factor is heat-stable, acid-stable, water-soluble, and dialyzable.


The action of venom and proteolytic enzymes on the non-specific inhibitor of hyaluronidase. 1. It has been shown that a number of proteolytic enzymes and snake venom, in relatively small amounts, and within a wide range of pH variation, will restore hyaluronidase activity after its inhibition by serum. 2. The known properties of the venom protease are found to be identical with those of Haas' "proinvasin I." It is concluded that the protease of the venom offers adequate explanation for the effects previously attributed to "proinvasin I." 3. Proteolytic activity is found in hyaluronidase preparations of bovine origin and is considered to be responsible for the reversal of inhibition of hyaluronidase by serum.


Investigations on the mitochondria of the house fly, Musca domestica L.  I.  Adenosinetriphosphatases. 1. The ATPase activity of insect mitochondria has been investigated. A comparison was made to determine the distribution and nature of such activity in other isolated fractions of the house fly, Musca domestica L. 2. The ATPase in insect mitochondria is specific in that orthophosphate can be cleaved only from ATP. The Michaelis-Menten constant K(8) = 2.78 x 10(-3)M and V(max.) = 76 micrograms P min.(-1) mg.(-1) dry weight. 3. Mg(++) and Mn(++) activate this enzymatic reaction in mitochondria, but Ca(++) does not. The extent of activation is 60 per cent with the optimal concentration 6 x 10(-4)M. Experiments with combinations of Mg(++) and Mn(++) show that either ion can replace the other and that the effects are additive, depending solely on the final concentration of the combination. Concentrations of Mg, Mn, or Ca ions higher than 6 x 10(-3)M inhibit the enzyme. 4. Fluoride does not inhibit the ATPase of insect mitochondria, whereas azide and chloromercuribenzoate do. The per cent inhibition depends on the concentration of inhibitor. 5. Finely dispersed mitochondrial particles have much greater ATPase activity than intact mitochondria. The possible relationship of this observation to latent ATPase is considered. 6. A magnesium-activated adenylate kinase is present in these mitochondria. The liberated orthophosphate, derived from ADP, is the result of the activity of adenylate kinase followed by the specific ATPase. 7. ATP can be dephosphorylated by enzymes found in the muscle fibrils, and in a "soluble" fraction, as well as in mitochondria. The fibrillar ATPase is Ca(++)-activated. The "soluble" fraction, however, like the mitochondria, is Mg(++)-activated. The "soluble" ATP dephosphorylation mechanism is distinguished from the mitochondrial ATPase in that it is inhibited by fluoride. 8. The "soluble" fraction also contains a magnesium-activated inorganic pyrophosphatase. Fluoride completely inhibits this enzymatic reaction. 9. The possible mechanism of ATP dephosphorylation in the "soluble" fraction is discussed.


Immunological studies on insect metamorphosis.  I.  Qualitative and quantitative description of the blood antigens of the Cecropia silkworm. 1. The cell-free blood of the Cecropia silkworm produces a maximum of nine bands of antigen-antibody precipitate when reacted in antiserum-agar tests with antisera prepared by injecting Cecropia extracts into rabbits. The blood antigens producing these bands of precipitate have the properties of proteins in that they are non-dialyzable, labile at 75 degrees C., and salted out by 75 per cent saturated ammonium sulfate. One antigen was identified as a carotenoid protein. 2. Six bands of precipitate were selected for further study. Absorption tests revealed that the blood, at all stages of metamorphosis, is capable of precipitating the antibodies which produce five of these bands. This result indicates that five of the six antigens are present in the blood throughout metamorphosis. The sixth antigen is undetectable in blood from fourth instar larvae, appears in the blood late in the fifth instar, persists during the pupal stage, and disappears again during adult development. 3. When blood samples from various stages of metamorphosis were tested in antiserum-agar tubes, the rates of advance of the six bands of precipitate underwent systematic change in close correlation with the morphological stage of the silkworm's metamorphosis. These changes are interpreted in terms of concentration changes of the corresponding blood antigens. The validity of this interpretation was tested in several ways, with the conclusion that the interpretation was generally acceptable for the system under consideration. 4. All six antigens appear to increase in concentration during the last larval instar and to decrease in concentration during the period of adult development. However, each antigen has its own characteristic pattern of concentration change which differs from those of the other five. In order to explain this diversity, we conclude that the physiological mechanisms which regulate the synthesis and utilization of the blood antigens control each antigen on an individual basis.


Geometrical isomers of retinene.

Geometrical isomers of retinene. Five crystalline retinenes have been isolated, which have every appearance of being cis-trans isomers of one another. They are all-trans retinene; three apparently mono-cis isomers: neoretinenes a and b and isoretinene a; and isoretinene b, an apparently di-cis isomer. The absorption spectra of these substances display the relations expected of cis-trans isomers. The main absorption band is displaced 5.5 to 7 mmicro toward shorter wave lengths for each presumptive cis linkage. Some of the presumptive cis isomers also display a cis peak at 255 to 260 mmicro. All five substances yield an identical blue product on mixing with antimony chloride. All of them are converted by light to what appears to be an identical mixture of stereoisomers. Heat isomerizes them very slowly; only neoretinene b exhibits large changes on heating at 70 degrees C. for 3 hours. The various isomers have been extensively interconverted by gentle procedures, and all of them have been converted to all-trans retinene. The present theory of cis-trans isomerism in carotenoids predicts the existence of four stable isomers of retinene. Instead we seem to have five-specifically three mono-cis forms where two are expected. There is no doubt that all these substances are closely related isomers of one another. The only point in question is whether they differ in part by something other than cis-trans configuration. One possibility, as yet little supported by evidence, is that isomerization between beta- and alpha-ionone rings may be involved. If, however, as seems more likely, all these substances are geometrical isomers of one another, some modification is needed in the present theory of configurational relationships in this class of compounds.


Characteristics of collodion membranes for ultrafiltration. An apparatus for the production of graded collodion membranes is described. The theoretical considerations of calibration are discussed in relation to the demonstrable structure and statistical characteristics of the membranes. A new definition of an "end-point" is suggested.


Effects of castration and the processes of aging in male rats and man.


Geographic and historical pathology of arteriosclerosis.


Long range sex steroid replacement; target organ regeneration.


The normal precordial electrocardiogram in the aged.


The effect of change of body position on the precordial electrocardiogram in young and aged subjects.


The potassium content of plasma and red cells in various age groups.


Thyrotoxicosis in aged individuals.

Thyrotoxicosis in aged individuals.


Present-day surgical viewpoints and procedures in vascular surgery of the aged; a review.


The Wechsler-Bellevue performance of white males past age 50.


Retirement and the professional worker.

Retirement and the professional worker.


Geriatric nursing programs in schools of nursing.


A community plans a recreation program for the aged.


Report of the fifth annual scientific meeting of the Gerontological Society, Inc.


Determination of beta-glucuronidase in microgram quantities of tissue and its distribution in the cow adrenal.


Stable sudanophilia of human neutrophil leucocytes in relation to peroxidase and oxidase.


Biochemical heterogeneity of the cytoplasmic particles isolated from rat liver homogenate.


Cytochemical observations on chicken monocytes macrophages and giant cells in tissue culture.


Comparative distribution of succinic dehydrogenase in six mammals and modification in the histochemical technic.


Variation of Bacillus anthracis with special reference to the non-capsulated avirulent variant.


The estimation in vitro of small amounts of diphtheria antitoxin by means of a haemagglutination technique.


Sociologic factors in the spread of epidemic hepatitis in a rural school district.


Nasal mucus and influenza viruses.  II.  A new test for the presumptive diagnosis of influenza infection.


Nasal mucus and influenza viruses.  I.  The haemagglutinin inhibitor in nasal secretions.


Studies on the susceptibility of Rickettsia burneti to chemical disinfectants, and on techniques for detecting small numbers of viable organisms.


The properties of different Salmonella Vi antigens.


The Vi antigen of Salmonella paratyphi B.


The Vi antigen of Salmonella paratyphi A.


The serological classification of Bacteriaceae.


Uptake of labeled antigens by the mitochondria of mouse liver.


Studies on immunization against plague.  IV.  The method of the hemagglutination test and some observations on the antigen.


Involvement of a complement-like factor in the activation of blood protease.


Electrophoretic studies on hyperimmune rabbit sera for neurotropic viruses.


The role of tubercle bacilli in adjuvant emulsions on antibody production to egg albumin.


Inhibition of the Shwartzman reaction by pyrogenic substances.


The quantitative determination of the extent of despeciation of modified equine antitoxin.


Studies on the cultivation of poliomyelitis viruses in tissue culture.  I.  The propagation of poliomyelitis viruses in suspended cell cultures of various human tissues.


General preface to studies on the cultivation of poliomyelitis viruses in tissue culture.


Treatment of chest injuries.

Treatment of chest injuries.


The carotid sinus reflex.  III.  Spontaneous and induced Stokes-Adams attacks in a patient with the hyperactive reflex.


Studies on the cultivation of poliomyelitis viruses in tissue culture.  II.  The propagation of the poliomyelitis viruses in roller-tube cultures of various human tissues.


Rabies, a threat to human health.

Rabies, a threat to human health.


The influence of age of the animal upon the susceptibility of mammalian erythrocytes to infection by the avian malaria parasite Plasmodium lophurae.


Further studies of the pathogenicity and immunogenicity of mucoid variants of Brucella abortus for guinea pigs.


Dream and fulfilment; the Surgeons' Hall of Fame of the International College of Surgeons.


Experimental transmission of Trichomonas vaginalis (pure culture) into mice.


Malignant disease of the urinary bladder.


Plastic reconstruction of the hip with nylon.


Duration of acquired immunity of Schistosomatium douthitti infections in mice following treatment.


The curative action of antimalarial drugs against Plasmodium lophurae in chicks.


Prevention of iatrogenic trauma in otolaryngology.


The effect of sodium copper chlorophyllin on growth and acid production of some oral bacteria.


Further experience with an operation for the cure of certain types of impotence.


Segmental resection in the surgical treatment of pulmonary tuberculosis.


A polysaccharide medium of known composition favoring chlamydospore formation in Candida albicans.


Tuberculostatic substances extracted from normal rat spleen.


Medical problems in disease of the gallbladder.


Surgical management of diverticulitis.

Surgical management of diverticulitis.


The nature of the sparing phenomenon.  II.  Activity in vivo and nature of the sparing substance.


Laboratory studies of Histoplasma capsulatum.  II.  Size of the spores.


Orthopedic treatment of acute and subacute poliomyelitis by early stretching with the aid of curare.


Causes of failure in surgical fenestration; a follow-up of 3,000 consecutive cases.


Chemotherapy of experimental leptospirosis with chloramphenicol, (chloromycetin), subtilin and penicillin G.


Growth response of microorganisms to vitamins at different temperatures.


Complications following vaginal hysterectomy.


Asphyxial accidents in surgery.

Asphyxial accidents in surgery.


The effect of ultraviolet irradiation on immune serum.


The effect of x irradiation on infections with Plasmodium berghei in the white mouse.


Studies on the chemical composition of human hair fat.  I.  The squalene-cholesterol relationship in children and adults.


Atabrine in the treatment of discoid lupus erythematosus.


Therapeutic assays of the Skin and Cancer Unit of the New York University Hospital.  Assay VII-Quinacrine hydrochloride (atabrine hydrochloride) for chronic discoid lupus erythematosus.


Flocculation reactions in rabbits experimentally infected with Trichinella spiralis.


Treatment of chronic discoid lupus erythematosus with atabrine.


Investigative studies on Colorado shale oil.


Studies of skin reaction to propylene glycol.


Examination of the epidermis by the strip method.  II.  Biometric data on regeneration of the human epidermis.


Electron microscopy of normal human skin.


A study of eczematous sensitibity to formaldehyde.


Sodium para-aminobenzoate PABA in the treatment of dermatoses.


The threshold of skin flare in persons with and without malignant neoplastic disease.


Experimental therapy of chronic porphyria with vitamin B12.


Specific tissue alteration in leprous skin.  II.  The histology of the tuberculin reaction in leprosy.


Allergic factors in the production of leukemids.


A hemolytic serologic reaction without complement as a possible test for syphilis.


The metal content of skin, nails and hair.


Water soluble vitamins in normal human skin.


A note on fingernail growth.

A note on fingernail growth.


Studies of skin-hypersensitivity to lanolin.


Jaundice.

Jaundice.


Histopathologic studies of pigmented nevi in children.


Effects of the resin of Euphorbium on verrucae plantares; human and animal experimentation.


Present concepts of silent gallstones.

Present concepts of silent gallstones.


The use of metallic elements in repair of hernia defects.


Education for childbirth in private practice; 669 consecutive cases.


Secondary hemorrhage postadeno-tonsillectomy.


CLINICAL pathologic conference:  tuberculosis, active:  kidneys, ureters, urinary bladder, uterine cervix; nephrostomy, left, postoperative; peritonitis, pelvic.


A plea for local care of crippled children and handicapped adults.


Address before the Fifth Annual Public Relations Conference.


Rodent control.

Rodent control.


Some common obstetrical problems and their management.


Management of the persistent occiput posterior position.


Vaginal hysterectomy in the treatment of prolapse.


The midforceps operation.

The midforceps operation.


CLINICAL pathologic conference:  osteogenic sarcoma involving the entire right thoracic cage, vertebrae, sternum, diaphragm and right lung; metastasis to left lung.


EVOLUTION of the GP specialists.

EVOLUTION of the GP specialists.


Arthur Wright Erskine, M.D., 1885-1952.

Arthur Wright Erskine, M.D., 1885-1952.


The wheezing patient; a symposium.

The wheezing patient; a symposium.


Channing Gamalial Smith, M.D., 1877-1952.


Skulkers unveiled:  or further notes on malingering.


Isoniazid in pulmonary tuberculosis.

Isoniazid in pulmonary tuberculosis.


Bornholm disease and the Coxsackie viruses.


That a liberal education is necessary for a medical student.


The possibilities and limitations of isotope therapy of cancer.


Skin injury by flourescent bulb.

Skin injury by flourescent bulb.


The complications and diagnosis of infectious mononucleosis.


AMERICAN Medical Education Foundation.

AMERICAN Medical Education Foundation.


Senior thesis from the university of Kansas medical school; sarcoidosis.


Case reports from the University of Kansas Medical Center tumor conference:  germinal tumors of the testis.


The management of terminal patients with inoperable carcinoma.


Treatment of thyroid carcinoma with radioactive iodine.


CLINICAL pathological conference:  pulmonary fibrosis secondary to chronic, indolent pneumonia.


Interpretation of the blood sedimentation rate.


The evaluation of operative risk in patients with cancer.


Pregnancy complicated by spina bifida, neurological bladder and pyelonephrosis.


MINIMUM standards of obstetrical care.

MINIMUM standards of obstetrical care.


Your school of medicine.

Your school of medicine.


How to win patients and influence doctors.


CASE reports from the University of Kansas Medical Center; tumor conference; carcinoma involving the esophagus.


A.M.A. interim session.

A.M.A. interim session.


Reporting communicable disease.

Reporting communicable disease.


BLUE Shield; waiting periods.

BLUE Shield; waiting periods.


The use of isoniazid in the treatment of tuberculosis.


Geriatric surgery; some observations of surgical patients in the older age groups.


When a psychiatric case walks into your office.


Considerations of the surgical treatment of carcinoma of the cervix uteri.


Histoplasmosis.

Histoplasmosis.


Seasonal incidence of acute myocardial infarction in the Kentuckiana area.


The doctor's day in court.

The doctor's day in court.


Complications of biliary tract surgery.

Complications of biliary tract surgery.


The general practitioner sees the hypertensive patient.


Rural general practice, 1952.

Rural general practice, 1952.


Our geriatric problem.

Our geriatric problem.


Practical aspects of the treatment of purulent meningitis in children.


Urinary stress incontinence in women.

Urinary stress incontinence in women.


Traumatic diaphragmatic hernia with acute intestinal obstruction; a report of three cases.


Kentucky-the progenitor of pioneer doctors.


WOULD you like to be a councilor?

WOULD you like to be a councilor?


Teachers of medicine.

Teachers of medicine.


The relation of life stress and emotions to human sebum secretion and to the mechanism of acne vulgaris.


An evaluation of the measurement of the cardiac output and of the so-called pulmonary blood volume by the dye-dilution method.


Effect of prolonged administration of radioactive zinc 65 on the pancreas.


Excretion of water and electrolytes in patients with essential hypertension.


The use of cobaltous chloride in the anemia associated with chronic renal disease.


Maintenance therapy of pernicious anemia with vitamin B12.


The combined anticonvulsant activity and toxicity of dilantin and N-methyl-5-phenylsuccinimide.


Post-transfusion survival of erythrocytes stored in a solution of ethylenediamine tetraacetic acid and dextrose.


Variation in complement fixation antigen production by different strains of Histoplasma capsulatum grown on two media.


An evaluation of the Middlebrook-Dubos hemagglutination test.


The migration and oxidation-reduction activity of leukocytes from patients with acute disseminated lupus erythematosus.


The role of cold hemagglutinins in frostbite.


On the duration of penicillin action in relation to its concentration in the serum.


Clinical laboratory studies in experimentally-induced epidemic nonbacterial gastroenteritis.


Recent changes in sensitivity of Micrococcus pyogenes to various antibiotic agents.


Prevention of paralytic poliomyelitis in tonsillectomized cynomolgus monkeys by human gamma globulin.


Quantitative determination of nitrate by enzymic reduction to nitrite.


Identification of abnormal hemoglobins by means of paper electrophoresis.


An accumulating impulse counter.

An accumulating impulse counter.


Respiratory air currents and their clinical aspects.


Interstitial irradiation of the larynx; a report of eight cases.


A case of multiple rhinosproridiosis.

A case of multiple rhinosproridiosis.


A method of dilation of strictures of the oesophagus.


One stage closed fenestration method, using conjunctival graft.


Neurofibroma of the facial nerve; excision with nerve graft.


The sense of hearing.

The sense of hearing.


Anaesthetic problems in otorhino-laryngology.


Benjamin Guy Babington, M.D., F.R.C.P., F.R.S., 1794-1866.


A description of Babington's disease (hereditary multiple telangiectasia), with the report of a case.


Tuberculoma of the tongue.

Tuberculoma of the tongue.


BRITISH Association of Otolaryngologists; annual report of Council, 1951-52.


A new monocular ear microscope.

A new monocular ear microscope.


Spinal headache.

Spinal headache.


Surgery in deafness.

Surgery in deafness.


En bloc surgery for intraoral cancer.

En bloc surgery for intraoral cancer.


What the general practitioner should know about crossed eyes.


Otitis externa.

Otitis externa.


Spontaneous rupture of the esophagus.

Spontaneous rupture of the esophagus.


Creeping eruption (larva migrans) at Keesler Air Force Base.


Cutaneous blastomycosis; a report of fifty-eight unpublished cases.


Current therapy of several common skin diseases.


Quo Vadis.

Quo Vadis.


CHEST pain associated with pulmonary hypertension.


Appendicitis; a clinical and pathological study.


Third stage of medicine.

Third stage of medicine.


General principles regarding the treatment of sinusitis.


Neuro-surgical aid to the general practitioner.


Infantile cortical hyperostosis; case report.


Pulmonary hemorrhage associated with Boeck's sarcoid and mitral stenosis; case report.


JAMES W. Crane, M.D., 1879-1952.

JAMES W. Crane, M.D., 1879-1952.


[A form of ectodermal dysplasia; hypotrichosis with anhidrosis and anodontia].


The influence of the American Academy of General Practitioners on American medicine.


Poliomyelitis, pathogenesis and prophylaxis with use of gamma globulin.


The Georgia Postmortem Examination Act.

The Georgia Postmortem Examination Act.


PROPOSED "injunction law".

PROPOSED "injunction law".


Problems in weight bearing and gait in poliomyelitis.


Meckel's diverticulum as a cause of intussusception.


Treatment of alcoholic.

Treatment of alcoholic.


Non-penetrating injuries of the abdomen.


MEDICAL Association of Georgia:  constitution and by-laws.


MEDICAL Association of Georgia:  roster of membership, December 31, 1952.


WOMAN'S Auxiliary to the Medical Association of Georgia:  roster of membership, December 31, 1952.


From the president of the Georgia Heart Association.


Vocational rehabilitation of cardiac.

Vocational rehabilitation of cardiac.


The management of auricular fibrillation.


The treatment of severe arterial hypertension with hexamethonium and hydrazinophthalazine.


The low salt syndrome and its treatment.


Does exertion precipitate coronary thrombosis?


Prognostic and therapeutic implications of classification of hypertensive disease.


Intractable heart failure.

Intractable heart failure.


The place of the cardiac clinic in relation to the public health program of heart disease control.


PROGRESS report of the Georgia Heart Association.


Asterol treatment of superficial fungus infections.


Current treatment of tuberculosis.

Current treatment of tuberculosis.


Respiratory urography.

Respiratory urography.


Total pelvic evisceration; report of four cases.


Pediatric case reports.

Pediatric case reports.


The enemies of child health.

The enemies of child health.


Lead poisoning.

Lead poisoning.


PROVISIONAL birth and death statistics for July 1952, and comparative rates.


Management of congestive heart failure refractory to usual treatment.


Surgical management of gastric and duodenal ulcers.


Carcinoma of the colon involving the abdominal wall and other viscera.


Danger in influenza.

Danger in influenza.


Ultrasonics in osteoarthritis.

Ultrasonics in osteoarthritis.


Genito-urinary disease as a cause of non-urologic complaints.


Treatment of chronic cervicitis.

Treatment of chronic cervicitis.


Saddle block anesthesia in obstetrics.

Saddle block anesthesia in obstetrics.


Observations on the mechanism of glaucoma and lens nutrition.


Hemolytic disease of the newborn.

Hemolytic disease of the newborn.


Aedes aegypti surveys in three cities in Alabama in 1952.


The financial status of medical education.


The study of applicants.

The study of applicants.


Are there principles in teaching physiology?


Human physiology for first-year medical students.


Report of student committee on the medical students' point of view.


The teaching of physiology to third and fourth-year medical students.


The point of view of practicing physicians toward teaching physiology in medical schools.


Concepts of the short film with reference to medical school classroom teaching.


The development of the short film in medicine.


Double value; the planned production of simultaneous long and short medical motion pictures.


Short films for cancer teaching in the medical school; report of a study in progress aspects of production.


The implications of short films in the medical school; film production, distribution and utilization.


APPLICANT studies.

APPLICANT studies.


UNSUCCESSFUL applicants.

UNSUCCESSFUL applicants.


[An experience of antituberculosis prophylaxis organization in the Middle East].


[Hydrazide in treatment of pulmonary tuberculosis].


[First results of the treatment of pulmonary tuberculosis with isonicotinic acid hydrazide in the sanatorium of Bhannès].


Chemotherapy in miliary tuberculosis.

Chemotherapy in miliary tuberculosis.


An example of a successful social approach to tuberculosis control.


[Role of antibiotics in the treatment of osteoarticular tuberculosis; results of our experiences with 360 cases].


The treatment of chronic pharyngeal infection and postnasal drip in children.


Premature baldness; preliminary report on treatment with topically applied estrogen lotion.


The public health aspects of cytology in cancer detection.


Reticulocytosis following ACTH in sickle cell anemia; case report.


Isoniazid in the treatment of tuberculosis.


Speech handicapped children.

Speech handicapped children.


Today's tuberculosis problem in Arizona, a study of patients' origins.


Carbohydrate disturbances in liver disease.


Evaluation of functional murmurs in children.


It's nice money if you can get it.

It's nice money if you can get it.


Ataxic cerebral palsy with akinetic seizures; dramatic response to dramamine.


Hodgkin's disease in a Negro boy.

Hodgkin's disease in a Negro boy.


The nature of psychotherapy.

The nature of psychotherapy.


Cardiovascular surgery at St. Michael's Hospital.


JUDICIAL Council:  on fee splitting.

JUDICIAL Council:  on fee splitting.


Pregnancy complicated by diaphragmatic hernia.


Reflections on genius.

Reflections on genius.


Personality and visual perception:  a review.


Genetic investigations in twins.

Genetic investigations in twins.


A comparison of the personality changes after prefrontal selective surgery for the relief of intractable pain and for the treatment of mental cases; cingulectomy and topectomy.


Psychosomatic aspects of the premenstrual tension syndrome.


An experimental inquiry into some aspects of the motor behaviour and personality of tiqueurs.


Gargoylism, Hurler's disease a neuropathological report.


Occupational psychiatry:  an historical survey and some recent researches.


The use of electroplexy in mental disease with clouding of consciousness.


A special mental hospital unit for the treatment of psychosis and neurosis in juveniles.


Electrical termination of hypoglycaemic coma.


Human behaviour reactions to organic cerebral disease.


Pictorial art, viewed from the standpoint of mental organization as revealed by the excitatory abreactions techniques of psychiatry.


Acute porphyria presenting with mental symptoms.


The routine use of muscular relaxants prior to electrical convulsive therapy.


Observations on the short-acting relaxant RO.3.0386.


Report on short-action muscle relaxant RO.3.0386 in E.C.T.


Celastrus paniculata in mental deficiency:  a pilot experiment.


The importance of history and physical examination in the diagnosis of heart disease.


Treatment of the nephrotic syndrome.

Treatment of the nephrotic syndrome.


The surgical treatment of mitral stenosis.


Current treatment of rheumatic fever.

Current treatment of rheumatic fever.


Studies with the flicker photometer.

Studies with the flicker photometer.


A simplified technique for wiring aneurysms.


Metastatic sarcoma of the heart diagnosed antemortem.


Hereditary capillary fragility.

Hereditary capillary fragility.


Myocardial infarction; comparison of results following use of dicumarol and dicumarol with heparin in the acute stage.


Cat-scratch disease.

Cat-scratch disease.


Re-enforcement of vascular grafts with intestinal musculature.


Frank N. Wilson-in memoriam.

Frank N. Wilson-in memoriam.


EARLE A. Irvin, M.D., Detroit, President of Industrial Medical Association.


HARRY Monroe Nelson, M.D., Detroit, President, American Cancer Society.


The Doctor William Beaumont memorial on Mackinac Island.


To build a medical shrine, our privilege.


Financing the Beaumont memorial.

Financing the Beaumont memorial.


The Beaumont house its background and setting.


Portrait of Beaumont artist at work.

Portrait of Beaumont artist at work.


The future of the Beaumont memorial.

The future of the Beaumont memorial.


Exfoliative cytology in periodical physical examinations; progress report.


Preventive medicine in the general practitioner's office.


Dysphagia.

Dysphagia.


The problems and management of the fractured hip.


Large dermoid cyst of ovary obstructing birth canal as a complication of pregnancy.


HEALTH care of the nation.

HEALTH care of the nation.


Arthritis; how it affects the feet.

Arthritis; how it affects the feet.


Surgical treatment of hallux valgus.

Surgical treatment of hallux valgus.


Delivery of thoracopagus twins.

Delivery of thoracopagus twins.


Report on an acid treatment removal of verrucae among service personnel.


CHIROPODOLOGIA, a scientific enquiry into the cause of corns, warts, etc.


CHIROPODY service in a hospital.

CHIROPODY service in a hospital.


HOSPITAL departments of dentistry.

HOSPITAL departments of dentistry.


Insurance claim recognition; prepaid health and accident insurance and the chiropodist.


"Painful feet" in American prisoners of war.


VACATION athletes told to "take it easy" by American Heart Association.


Inaugural address.

Inaugural address.


Heart disease in relation to employment.


Control of post-extraction hemorrhage.

Control of post-extraction hemorrhage.


Low frequency current in diagnosis and therapy.


Total rehabilitation.

Total rehabilitation.


Medical care organizations.

Medical care organizations.


Diagnostic roentgenology in obstetrics and gynecology; a review of basic considerations.


A ten-year survey of gall bladder surgery at Provident Hospital.


The narcotic problem in an urban community.


Culdoscopy; its diagnostic value in disorders of the female pelvis.


The detection and management of early toxemia of late pregnancy.


Closed treatment of carcinoma of bladder.


An anaphylactoid reaction due to oral penicillin:  a case report.


HERBERT CLAY SCURLOCK.

HERBERT CLAY SCURLOCK.


CHARLES BURLEIGH PURVIS.

CHARLES BURLEIGH PURVIS.


Aphasia.

Aphasia.


The effects of methedrine and of lysergic acid diethylamide on mental processes and on the blood adrenaline level.


Investigation of amnesic defects by progressive prompting.


The paraesthesiae induced by cold.

The paraesthesiae induced by cold.


Affective epilepsy.

Affective epilepsy.


The epileptic threshold in schizophrenia.


Disturbances of oculomotor function accompanying extradural haemorrhage.


The intra-arterial injection of neostigmine as a diagnostic test in myasthenia gravis.


The neurological aspects of dermatomyositis.


Tumors of the posterior portion of the third ventricle.


Painful spina bifida occulta; with review of the literature.


Studies on cerebral intravascular pressure; further data concerning residual pressure in components of the circle of Willis and in blind arterial segments.


The effect of contrast media on the blood-brain-barrier.


The aneurysmal origin of nonfatal subarachnoid hemorrhage; an angiographic survey of 53 cases.


The cerebral circulation in postoperative intracranial hypotension.


The duration of neuromuscular function after nerve section in man.


Personal experience in the surgical treatment of epilepsy.


Cerebral calcification epilepsy; report of a case of epilepsy caused by a calcified hamartoma of the brain.


A case of brain tumor with unilateral elevation of retinal artery pressure.


Medulloblastoma with onset during the neonatal period; report of a case.


Intradural lipoma of the spinal cord.

Intradural lipoma of the spinal cord.


Sir Hugh Cairns.

Sir Hugh Cairns.


Influence of the level of vitamins in the diet on the concentration of free amino acids in the plasma of chicks.


Gustav B. von Bunge, January 19, 1844-November 5, 1920.


A quantitative study of rumen synthesis in the bovine on natural and purified rations.  I.  Protein, dry matter and non-protein nitrogen.


A quantitative study of rumen synthesis in the bovine on natural and purified rations.  II.  Amino acid content of mixed rumen proteins.


Effects of prolonged percutaneous administration of methyl testosterone and estradiol on growing male rats.


Folic acid deficiency in the duck.

Folic acid deficiency in the duck.


Zinc deficiency in suckling mice deprived on colostrum.


Dermatosis in weanling rats fed lactose diets.  II.  Histological studies.


Rat growth assay for vitamin B12; correspondence with collaborative microbiological assay results on U.S.P. liver extracts.


Experimental megaloblastic anemia and scurvy in the monkey.  V.  Nature of the relation of ascorbic acid deficiency to the metabolism of folic acid compounds.


Effects of dietary aureomycin upon the intestinal microflora and the intestinal synthesis of vitamin B12 in the rat.


Comparison of four feeding methods for assessing the relative growth-promoting properties of proteins.


Growth response of the rat to heterologous protein administered intraperitoneally.


Cobalt and vitamin B12 in sheep.  I.  Distribution of radiocobalt in tissues and ingesta.


The metabolic fecal nitrogen excretion of the young calf and the true digestibility of casein.


Measurement of accommodation in dim light and in darkness by means of the Purkinje images.


The scanning x-ray microscope.

The scanning x-ray microscope.


Photopic thresholds for red light in an unselected sample of color-deficient individuals.


Duration and light-dark ratio of intermittent preadaptation as factors influencing human dark adaptation.


Three-dimensional color models constructed on the CIE and Munsell systems.


Fibrous (fibro-osseous) dysplasia of jaw bones.


Probenecid-penicillin in oral surgery.

Probenecid-penicillin in oral surgery.


Terramycin in the treatment of cellulitis of the floor of the mouth.


Methantheline bromide as premedication for oral surgery.


The dentist and the explosion hazard.

The dentist and the explosion hazard.


Present concept of oral focal infection.


Oral manifestations of infection with the virus of herpes simplex.


Correction of asymmetric prognathism by unilateral ostectomy.


A method of immobilizing a common type of maxillary fracture.


Technic for creating an emergency airway in cases of respiratory obstruction.


Writing for reading.

Writing for reading.


Malignant transformation of odontogenic cyst:  report of case.


Sequestrectomy and immediate bone graft in osteomyelitis of the mandible following fracture:  report of case.


Extensive spontaneous opening of the antrum following extraction of a tooth:  report of case.


Pledges continued attention to fullest accomplishment of purposes of Kirksville College of Osteopathy and Surgery.


FOCAL infection as viewed by the oral surgeon.


COLLEGE at Kirksville observes Founder's Day with full day of activities; honors graduates.


Nemathelminthic infections.  I.  Rhabditida nematode parasite of man, Enterobius Vermicularis (Linnaeus, 1755) Leach, 1853, and Enterobiasis, together with a brief review of the current literature on therapy...abstract report of a case with appendicial involvement.


Laminated trichobezoar; a case report.

Laminated trichobezoar; a case report.


Laboratory procedures and interpretations; liver function tests.


Acceptance of osteopathic heritage means beginning of a greater service; medicine as a service will not be limited by persons activated primarily by devotion to strict organization.


Typhoid fever; a case report.

Typhoid fever; a case report.


AUDRAIN County Hospital decision upheld by St. Louis Court of Appeals:  osteopathic physicians declared within meaning of law of state and entitled to practice in county hospitals.


A living heritage.

A living heritage.


Chronic myelogenous leukemia.

Chronic myelogenous leukemia.


Parasitemia in primary Leucocytozoon simondi infections.


Potential molluscacides screened in the laboratory and the results of preliminary field plot tests.


Studies on bovine gastrointestinal parasites.  XIX.  Further studies in the use of small amounts of phenothiazine in pure infections of the nodular worm.


The coccidiostatic action of nitrophenide on Eimeria tenella.