On a differentiating mechanism of psychosomatic disorder.


An investigation into the psycho-analytic theory of depression.


A survey of 1,100 stillbirths.

A survey of 1,100 stillbirths.


The predisposing situation to peptic ulcer in children.


The artificial kidney.

The artificial kidney.


Diabetes mellitus in Ireland.

Diabetes mellitus in Ireland.


Birth and growth of a specialty.

Birth and growth of a specialty.


The changing face of paedriatrics.

The changing face of paedriatrics.


Haemorrhagic thrombocythaemia.

Haemorrhagic thrombocythaemia.


The effects of x-rays, water content and temperature on growth.


Studies on the experimental typhoid. II. Relation between virulence and antigenic structure of virulent and attenuated strains of S. enteritidis.


Cell-like bodies prepared from mixtures of egg white and oil.


The occurrence and distribution of Salmonella and Arizona in Japan.


Radiculoneuritis due to antivenom serum treatment after snake bite.


Health problems in the absorption of mass immigration.


Caterpillar dermatitis.

Caterpillar dermatitis.


Kanamycin in bacillary dysentery.

Kanamycin in bacillary dysentery.


Recent tudies on the venom of Vipera xanthina palestinae.


Protein requirements of rat ascites hepatoma cells in tissue culture.


Cat scratch disease and its significance in a tumor clinic.


Prevention of Borrelia duttonii, Trypanosoma gambiense, Spirillum minus and Treponema pallidum infections conveyable through transfusion.


Production of alpha toxin of Clostridium perfringens. II. Failure to produce a potent alpha toxin in the simplified media.


Production of alpha toxin of Clostridium perfringens. III. The role of certain unidentified factors on the toxin production.


A case of multiple infection with Taenia saginata.


A histological study of the development of the urticating spicules of the Far Eastern urticating moth, Euproctis flava Bremer Lepidoptera: Lymantriidae).


The local difference of the resistance of Oncomelania nosophora, the vector snail of Schistosoma japonicum, to the desiccation.


Further studies on the 57-67 virus.

Further studies on the 57-67 virus.


Growth of vaccinia virus in HeLa cells with special reference to the production of soluble antigens.


Epidemiological studies of an outbreak of epidemic keratoconjunctivitis in Ogaki City and its vicinity, Gifu Prefecture in 1957.


Diphosphopyridine nucleotidase of murine leprosy bacilli.


Peripheral effector mechanism of galvinic skin reflex.


Electrical activity of single internode.


An analysis of potentials recorded intracellularly from the spinal ganglion cell.


The mode of impulse conduction through the spinal ganglion.


Further studies on the effects of the transection of the brain stem upon the respiratory movements.


The intrinsic reflexes in the colon.

The intrinsic reflexes in the colon.


Free amino acids in the choroid and effects of some metabolites on the retinal pigment migration.


Further study on the origin of the so-called cone action potential (s-potential); its histological determination.


Accommodation and local response in motoneurons of toad's spinal cord.


Functional organization of the cyprinid fish retina as revealed by discriminative responses to spectral illumination.


Microelectrode studies on the evoked activity of a single pyramidal tract cell in the somato-sensory area in cats.


Role of carbonic anhydrase in the bicarbonate excretion from salivary glands and mechanism of ionic excretion.


[Ricardo Jorge, teacher of epidemiology].


The nature of hypnosis: artifact and essence.


Drive strength and symptom manifestations.


Verbral rate, content, and adjustment ratings in experimentally structured interviews.


Hostility in Rorschach content and overt aggressive behavior.


The generality of social perception scores.


Subjective certainty and resistance to change.


The effects on opinions of a change in scale judgments.


Structural analysis of dreams; clues to perceptual style.


Autonomic responses and their relationship to race attitudes.


Effect of changes in self-esteem upon impulsiveness and deliberation in making judgments.


Stability and validity of achievement fantasy.


Need affiliation and frequency of four types of communication.


Re-evaluation of choice alternatives as a function of their number and qualitative similarity.


Increasing cognitive dissonance by a fait accompli.


Choice and change relative deprivation as determinants of cognitive dissonance.


Attitudinal effects of choice in exposure to countepropaganda.


Evaluation of therapy by self-report: a paradox.


Displacement of preference as a function of avoidance-avoidance conflict.


Personality, perceptual defense, and stereoscopic perception.


The relationship between GSR and speech disturbances in psychotherapy.


Some factors in the selection of leaders by members of small groups.


Yielding, authoritarianism and authoritarian ideology regarding groups.


New developments in anesthesiology.

New developments in anesthesiology.


Microbiology.

Microbiology.


New and useful drugs.

New and useful drugs.


Recent and current research in pedodontics.


Nothing to fear but complacency.

Nothing to fear but complacency.


Studies in dental radiology.

Studies in dental radiology.


Reaction of the human pulp to cavity preparation: results produced by eight different operative grinding technics.


Survey of dental health of the naval recruit. I. Status of dental health.


The pulpless tooth in rehabilitation of the mouth.


Solder joints on rustless alloys.

Solder joints on rustless alloys.


What I expect from my career in dentistry.


ADMISSION requirements for nonresident dentists to United States dental school degree programs; requisites for licensure and practice.


AN INVENTORY of dentists: age, membership, type of practice, dental school and location.


SURVEY of the dental laboratory industry. I. The dental laboratories responding.


The regulation of dental laboratories and technicians in Canada.


1958-59 LEGISLATIVE activities of the so-called public denturists.


The first century.

The first century.


The Association and dental science. I. Introduction.


A synoptic history of the American Dental Association.


The Association and dental science. 2. Physical research.


The Association and dental science. 3. Biological research.


The Association and dental science. 4. Dental therapeutics.


The Association and dental science. 5. Economic research.


The Association and dental education.

The Association and dental education.


National Board of Dental Examiners.

National Board of Dental Examiners.


The American Association of Dental Examiners.


The relief fund; a benevolent program.

The relief fund; a benevolent program.


Process patent litigation.

Process patent litigation.


Dentistry tomorrow.

Dentistry tomorrow.


Equipment research today and tomorrow.

Equipment research today and tomorrow.


An architect re-evaluates food service systems.


Dietary check sheet to aid administrators.


Its importance in sanitation, safety, and quality food service; preventive maintenance of equipment and utilities.


Cooking choice grade top round beef roasts; eff, of size & internal temperature.


Evaluating protein quality for advertising.


Effect of cooking method on vegetables; ascorbic acid retention & color differences.


Silver tableware through the ages.

Silver tableware through the ages.


PANEL discussion on chemotherapy in the palliative treatment of cancer.


A new stimulant supplement for the geriatric patient.


Simple surgical therapy for operable carcinoma of the mammary gland.


Treatment of nocturia in the elderly with posterior pituitary snuff.


Prochlorperazine in the management of restive aged patients.


Enzymatic factors in protein malnutrition of the geriatric patient.


Chemosurgery for brain tumor: a preliminary report.


Neurosurgical relief of intention (cerebellar) tremor; a preliminary report.


Rehabilitation of chronically ill geriatric patients: adjunctive use of an ataractic and nutritional-hormone preparation.


Host-tumor antagonism. XII. Possible effect of the cortisone drugs on the development of metastases in patients with carcinoma.


PANEL discussion on clinical nutrition and lipid metabolism.


Examination of an electrocardiogram.

Examination of an electrocardiogram.


The treatment of subacute asthma in the older patient.


An evaluation of orphenadrine hydrochloride (disipal).


Leydig-cell hyperplasia in elderly men.

Leydig-cell hyperplasia in elderly men.


Effect of a practical dietary regimen on serum cholesterol level.


Air hygiene for hospitals. II. Efficiency of fibrous filters against staphylococcic droplet nuclei and bacteria-bearing dust.


Poisonous snake bites; report of a case.


Medical care under the Health Insurance Plan of Greater New York.


A small community hospital handles a disaster.


Medical responsibilities in a changing world.


Why this conference.

Why this conference.


The rise of specialism in modern society.


Specialization and the pursuit of excellence.


The natural history of specialism in medicine.


The challenge of specialization in basic medical science.


The effect of specialization of graduate education in surgery.


Maintenance of standards in graduate medical education.


Workshop conference on specialism in medicine. I. Residence program.


Workshop conference on specialism in medicine, II. The specialty board.


The role of medical education in civil and defense mobilization.


Workshop conference on specialism in medicine. III. The resident and fellow.


Workshop conference on specialism in medicine. IV. The hospital.


NOMENCLATURE of blood clotting factors; four factors, their characterization and international number.


PROGRESSIVE pulmonary insufficency in a 57-year-old woman.


The problem of malignancy in nodular goiter: recapitulation and a challenge.


Pulmonary infarction after dental extraction: report of two cases with recovery.


Filtration versus local formation of lipids in pathogenesis of atherosclerosis.


The clinical radiologist and the problems of radiation hazards.


Research and training.

Research and training.


Research orientation and methods: contribution to educational objectives of the basic medical sciences.


Contribution of research orientation and methods to educational objectives of clinical sciences.


The role of research in graduate medical education.


Research and the teacher of medicine.

Research and the teacher of medicine.


Research orientation and the practitioner of medicine.


Maintenance of the synergistic relationship of education and research in medicine.


Major factors involved in reconstructing the medical curriculum.


Compression fractures of the thoracic vertebrae in a patient with tetanus.


An adequate diet.

An adequate diet.


Museums specialize too; for physicians and the public.


Thyroid nodules and the fallacy of all or none teaching.


VENTRICULAR fibrillation in hearts too good to die.


Study of surgically excised pulmonary granulomas.


The Educational Council for Foreign Medical Graduates.


Vascular accidents to the brain stem associated with neck manipulation.


Study of patients with hip arthroplasty at Massachusetts General Hospital.


Intradermal immunization against Asian influenza in children.


Lipid studies in health and disease; preliminary report of the results of lipid determinations in a group of normal male officers and patients with vascular diseases.


Experiences with general anesthesia in patients with familial dysautonomia.


Effect of amphetamine sulfate on athletic performance.


Amphetamine sulfate and athletic performance. I. Objective effects.


Use of amphetamines in athletics.

Use of amphetamines in athletics.


MEDICAL licensure.

MEDICAL licensure.


ANESTHETIC dangers in hereditary diseases.


MEDICAL licensure statistics for 1958; fifty-seventh annual presentation of licensure statistics by the Council on Medical Education and Hospitals of the American Medical Association.


The medical technologist and the doctor.


Polyps of the colon and rectum; a four-year to nine-year follow-up study of five hundred thirty-seven patients.


Staphylococcic pneumonia.

Staphylococcic pneumonia.


Fecal impaction in modern practice.

Fecal impaction in modern practice.


Ways and means to reduce infant mortality due to suffocation; importance of choanal atresia.


Duration of seroimmunity after injection with ultraviolet-irradiated poliomyelitis vaccine; role of booster inoculations.


Management of arteriosclerosis obliterans of the extremities.


Familial periodic disease with renal damage.


Hazards to the host of benign intrathoracic tumors.


Hematological safeguards during treatment with the phenothiazine derivatives.


Torsion of the gallbladder.

Torsion of the gallbladder.


Unusual foreign body (Steinmann pin) in bladder.


NEW AND nonfficial drugs: dithiazanine iodide.


The Joint A. M. A.-A. H. A. Committee on Professional Liability.


ACCIDENTAL iron poisoning in children.

ACCIDENTAL iron poisoning in children.


IS FAMILIAL periodic disease a nosological entity.


NUCLEAR sex.

NUCLEAR sex.


Health insurance for the aged.

Health insurance for the aged.


Health-welfare partnership.

Health-welfare partnership.


Common sense in the diagnostic use of x-ray.


Radiation dosage to female gonads during diagnostic roentgenographic procedures.


Cardiac catheterization in congenital heart disease.


Angiocardiography in diagnosis of congenital heart disease in infancy and childhood.


Use of the stethoscope in pediatric cardiology.


The course of active tuberculosis complicated by pregnancy.


Valsalva maneuver as a diagnostic aid.

Valsalva maneuver as a diagnostic aid.


The hands in arthritis.

The hands in arthritis.


Complete correction of the tetralogy of Fallot by open-heart surgery.


Gastroumbilical fistula developing after external blunt trauma to abdomen.


Steroid therapy in severe bacterial endocarditis; report of a case.


Eighth nerve deafness after administration of kanamycin.


State joint medicolegal committees.

State joint medicolegal committees.


NEW AND nonofficial drugs: cottonseed oil emulsion.


NEW AND nonofficial drugs: vancomycin hydrochloride.


Medical examiner versus coroner.

Medical examiner versus coroner.


COVERAGE and exclusions of professional liability insurance.


Use of orally administered live attenuated polioviruses as a vaccine in a community setting; a controlled study.


Communitywide vaccination program with attenuated poliovirus in Andes, Colombia.


Artificial hemodialysis in management of glutethimide intoxication.


Peritoneal dialysis. 1. Technique and applications.


Physiological and clinical effects of buccally given proteases.


Importance of hidden plus values in emergency resection for gastric hemorrhage.


Microbial sensitivity test in management of urinary tract infections.


Increasing seriousness of resistant urinary infections with Aerobacter aerogenes; current effectiveness of kanamycin.


Severe hypoglycemic shock due to chlorpropamide.


Calcium treatment for ineffective respiration resulting from administration of neomycin.


New splint for treatment of mallet finger.


The joint medicolegal education committee of the hospital.


Current status of therapy in glomerulonephritis.


Steroid therapy in endocrine disorders.

Steroid therapy in endocrine disorders.


MEDICOLEGAL aspects of head injuries.

MEDICOLEGAL aspects of head injuries.


BE A SPECIALIST, the easy way.

BE A SPECIALIST, the easy way.


Foundation for medical care.

Foundation for medical care.


SURVEY of professional liability insurance.


Pain in the chest and fear of heart disease; a student health physician's point of view of a common symptom complex.


Tolerance studies with a new contraceptive gel.


Attitude and response to treatment of adolescents and adults with dysmenorrhea.


Relationship between diseases in children and their pets.


Elise Strang L'Esperance.

Elise Strang L'Esperance.


CURRENT problems in professional relationships: a panel discussion.


Ectopic pregnancy.

Ectopic pregnancy.


The middle lobe syndrome.

The middle lobe syndrome.


Open method of burn treatment.

Open method of burn treatment.


Cardiac failure in the digitalized patient: a study of 42 cases.


Avulsion fractures.

Avulsion fractures.


Use of deanol in treatment of chronic fatigue and mild endogenous depression.


Herniations through the diaphragm.

Herniations through the diaphragm.


Effectiveness of the vaccine: a report on the surveillance of poliomyelitis in the United States, 1955 to 1958.


Drug addiction. II. Variation of addiction.


The spectrophotometric assay for chlortetracycline HCI and tetracycline HCI in pharmaceuticals.


Polarography of some phenylmercury amide and imide compounds.


Studies on antibacterial vapors of volatile substances.


The identification and determination of some official local anesthetics as tetraphenylborates.


In vitro test for antiarrhythmic agents.


Microestimation of opium alkaloids in pharmaceuticals by paper chromatography.


The biological action of cellular depressants and stimulants. I. The action of ethyl carbamate (urethane) on the endogenous respiration and the rate of cell division in Tetrahymena pyriformis.


Dialkylaminoethyl esters of 4-nitro- and 4-amino-3-hydroxy-2-naphthoic acids.


In vivo fibrinolytic effect of various proteolytic enzymes: quantitative tests employing iodine 131-labeled clots.


A note on the volatile oil from the fruits of Lomatium grayi.


Sabadilla alkaloids. VII. Sabatine and its alkamine sabine.


A radiometric method for determination of absorption of ammoniated mercury from ointments.


Interaction of preservatives with macromolecules. II. Correlation of binding data with required preservative concentrations of p-hydroxybenzoates in the presence of tween 80.


Interaction of preservatives with macromolecules. III. Parahydroxybenzoic acid esters in the presence of some hydrophilic polymers.


The determination of acetophenetidin in tablet mixtures: a potentiometric method.


Substituted alpha-benzylphenethylamines.


An improved method for the determination of lanthionine.


Amine derivatives of cyanuric chloride. IV. Catalytic hydrogenolysis of aromatically-bound chlorine.


Determination of rotenone in pharmaceuticals.


The effect of mescaline and yohimbine on the respiration of rat brain homogenate.


Diuretic activity of various compounds as determined by urinary excretion studies in rats.


Salts of p-acetamidobenzoic acid.

Salts of p-acetamidobenzoic acid.


16 alpha-Hydroxy steroids. I. Characterization of triamcinolone.


A study of the effect of some emulsifying agents on drug release from suppository bases.


Antimicrobial substances from seeds.

Antimicrobial substances from seeds.


The functional adduction of gait in childhood.


Analysis of barbiturate salts in various dosage forms by an ion exchange and nonaqueous titration procedure.


A note on the effects of gibberellins on alkaloidal content of Hyosyamus niger.


The amount of fines necessary for a tablet granulation.


In-toeing and out-toeing in infants and children.


Incidence of the secondary epiphysis of the first metatarsal.


Pediatric aspects of youth fitness.

Pediatric aspects of youth fitness.


Foot health report of a school health service.


Management of juvenile flatfoot.

Management of juvenile flatfoot.


Care of office emergencies.

Care of office emergencies.


The normal foot.

The normal foot.


An orthodigital splint for valgus of distal phalanx of hallux.


Foot mechanics and the x-ray.

Foot mechanics and the x-ray.


Roentgen diagnosis of short leg.

Roentgen diagnosis of short leg.


An approach to leg measurement.

An approach to leg measurement.


Diagnosis and treatment of local anesthetic reactions.


March fracture of the os calcis; a case report.


SURGICAL correction of retrocalcaneal bursa.


OPERATIVE technic for sinus condition and osteomyelitis.


A treatment for paronychia with concomitant onychocryptosis.


Surgical skin planing.

Surgical skin planing.


Survey and evaluation of the Veterans Administration podiatry program.


Experience with local vitamin A injection in the treatment of helomata and verrucae.


Prescribed home exercise; a case report.


A method of treatment for localized gangrene.


Surgical correction of pateilar ectopia in the dog.


Veterinary aspects of air pollution research.


Electrocardiography of tranquilized cattle. I.


A survey of swine brucellosis and leptospirosis in Wisconsin.


Bovine brucellosis cradication.

Bovine brucellosis cradication.


Prevalence of bovine coccidia in Wisconsin.


The effect on egg size and production, of fungicide-treated and fumigated grains fed to hens.


Ununited anconal processes of the dog.

Ununited anconal processes of the dog.


Torsion of the left lateral and papillary lobes of the liver in a pup; a case report.


The narcotic analgesics and their antagonists as adjuncts to barbiturate anesthesia. II. Clinical applications.


Promazine hydrochloride in equine practice.


Fatal occlusive pulmonary embolism in the dog; a case report.


Lungworms in a dog.

Lungworms in a dog.


Antibiotic sensitivity testing and bacteriophage typing of staphylococci found in the nostrils of dogs and cats.


Hydrogen sulfide poisoning of chinchillas.


Swine repopulation. I. Performance within a disease-free experiment station herd.


A serological survey for leptospiral antibodies in Maryland cattle.


A random serological survey for evidence of Leptospira pomona infection in Indiana cattle.


Pneumoretroperitoneum, a radiographic technique in the dog.


The relationship of hepatitis X of dogs and moldy corn poisoning of swine.


Promazine in canine medicine.

Promazine in canine medicine.


A calcium lactate aluminum hydroxide preparation as a preventive for parturient paresis.


Extraction of chlorine compounds produced by Streptomyces aureofaciens.


Relation of chlorine compounds produced by Streptomyces aureofaciens with production of chlortetracycline.


Structure of griseolutein A.

Structure of griseolutein A.


Production of tertiomycin A by Streptomyces albireticuli.


Some contributions to the chemotherapy of tuberculosis with viomycin from bacteriological aspect.


Uneven ventilation of normal and diseased lungs studied by an open-circuit method.


Estimation of inspiratory capacity in health and in subjects with respiratory muscle paralysis.


A new source of fradicinmycelin like antifungal antibiotic.


A method for dealing with data concerning uneven ventilation of the lung and its effects on blood gas transfer.


Comparison between alveolo-arterial gradient predicted from mixing studies and the observed gradient.


Effects of pure carbon monoxide gas injection into the peritoneal cavity of dogs.


Carbon monoxide determinations in post-mortem tissues as an aid in determining physiologic status prior to death.


Intrapulmonary mixing of gases studied with aerosols.


Physiological effects of chronic exposure to carbon monoxide.


Changes in acid-base balance and blood gases during muscular activity and recovery.


Studies on production of chlorteracycline and chlorine metabolism of Streptomyces aureofaciens.


Some effects of carbon dioxide on foreign gas uptake.


Effect of posture on pulmonary dead space in man.


In vitro effect of nonindole compounds on oxidative activity of ceruloplasmin.


Relative volume changes between lobes of the lung.


Combined ventilatory and breath-holding evaluation of sensitivity to respiratory gases.


Influence of vagal blockade on respiratory work in the hypoxic and hypercapneic anesthetized dog.


Tolerance to acute anoxia in high altitude natives.


Temperature thresholds for cutaneous pain.


Relationship between pain and tissue damage due to thermal radiation.


Tracking proficiency as a function of thermal balance.


Effects of oral glycine during cold exposure in man.


Free roaming' in the albino rat and its effect on restraint hypothermia.


Extreme warmth and sensorimotor coordination.


Effect of restraint on altitude tolerance in the rat.


EEG amplitude and reaction time during sleep.


Genesis of human cerebrospinal fluid.

Genesis of human cerebrospinal fluid.


Renal blood flow in dogs during diffusion respiration.


Passive antibody decay in rabbits under cold or altitude stress.


Electrical activation and mechanical asynchronism in the cardiac cycle of the dog.


Local vasodilator action of carbon dioxide on blood vessels of the hand.


Reproducibility of cardiac output measurements in the dog.


Effect of a vapor barrier on rates of excretion of sweat solutes.


Measurement of rates of excretion of sweat solutes under physiological conditions.


Distribution of fluoride in the normal dog femur.


Design of a body plethysmograph for studying cardiopulmonary physiology.


A method for quantitative estimation of rat erythrocytes in the mouse-rat chimera.


Effect of rhythmically inflating a pneumatic cuff at the ankle on blood flow in the foot.


Accuracy of an improved spirometer for recording of fast breathing.


Inaccuracy of the conventional water-filled spirometer for recording rapid breathing.


Graphical representation of respiratory gas dissociation characteristics of fetal and maternal blood.


Effects of partial and of total heart-lung bypass on the heart.


Two comments on the estimation of blood flow and central volume from dye-dilution curves.


Calibration of an aortic circumference gauge.


An electrical impedance spirometer.

An electrical impedance spirometer.


Calibration of a modified Riley bubble method for estimation of PCO2 and PO2 in whole blood.


Simple technique for portal-caval shunt in the rat.


A multiple-stage droplet oxygenator.

A multiple-stage droplet oxygenator.


An integrating cardiotachometer for optical or pen recording.


The milk alkali syndrom; a case report.

The milk alkali syndrom; a case report.


Testing of artificial heart valves.

Testing of artificial heart valves.


A lineoperated power supply for movable anode transducer tube.


Differential diagnosis of poliomyelitis.


Polarographic measurement of cerebral oxygenation using chronically implanted electrodes.


Current immunization procedures.

Current immunization procedures.


Toxemia of pregnancy.

Toxemia of pregnancy.


Diagnosis of renal disease.

Diagnosis of renal disease.


Diabetes mellitus.

Diabetes mellitus.


The Manhigh sealed cabin atmosphere.

The Manhigh sealed cabin atmosphere.


Exotic atmospheres on earth.

Exotic atmospheres on earth.


Reactions of a balloon crew in a controlled environment.


Physiologic responses to stressful stratosphere flights.


Closed respiration-ventilation system for use with high altitude full pressure garment.


Experiences with submarine atmospheres.

Experiences with submarine atmospheres.


Gastrointestinal microflora of antarctic birds.


Chemical composition of the granules of Mycobacterium-Thamnopheos, with special reference to their biological identity and the chemical nature of volutin.


Growth of obligate thermophiles at 37 C as a function of the cultural conditions employed.


Electrophoretic studies of sonic extracts of Proteus vulgaris. I. Effect of growth environment on electrophoretic patterns.


Simple means of obtaining concentration of influenza hemagglutinating antigen.


Mode of action of azaserine on Gaffkya homari.


Observations on Spirillum sputigenum and its relationship to Selenomonas species with special reference to flagellation.


Further studies on potentiation of inhibitor action through determination of reversing metabolites.


Influence of sorbic acid on the growth of certain species of bacteria, yeasts, and filamentous fungi.


Antigenic analysis of cell structures isolated from Bacillus megaterium.


Virulence in leptospirae. III. Comparison of sensitivities of virulent and of avirulent Leptospira icterohaemorrhagiae to cultural conditons.


Studies on the nutrition and physiology of Pasteurella pestis. IV. Utilization of peptides during the growth of Pasteurella pestis.


Mechanism involved in the metabolism of nitrophenyl-carboxylic acid compounds by microorganisms.


Comparative study of effect of temperature on metabolism of psychrophilic and mesophilic bacteria.


Mechanism of chemical mutagenesis. III. Induced mutations in spheroplasts of Escherichia coli.


Formation and multiplication of spheroplasts of Escherichia coli in the presence of lithium chloride.


Mechanism of hydrogen sulfide formation from thiosulfate.


Death of bacteria in growing culture.

Death of bacteria in growing culture.


Identity of the so-called vibrios of the oral flora.


Labeling of antibodies with fluorescent azo dyes.


Detection of typhoid carriers by agglutination tests.


Leptospira butembo; a distinct leptospiral serotype.


Studies on the chemoautotrophic iron bacterium Ferrobacillus ferrooxidans. I. An improved medium and a harvesting procedure for securing high cell yields.


Effects of chlortetracycline on the stability of arginine decarboxylase in Escherichia coli.


Sulfur incorporation into the alpha-amylase of Pseudomonas saccharophila.


Criteria for the identification of Bacillus anthracis.


Staining of coagulase-positive staphylococci with fluorescent antisera.


The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. II. Enzymic studies.


The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification.


The conversion of phosphoenolpyruvic acid and D-erythrose-4-phosphate to 5-de-hydroquinic acid.


2-Keto-3-deoxy-D-arabo-heptonic acid 7-phosphate synthetase.


Metabolism of the retina. I. Respiration of cattle retina.


L-2-Keto-4,5-dihydroxyvaleric acid: an intermediate in the oxidation of L-arabinose by Pseudomonas saccharophila.


The catalytic decarboxylation of pyruvate by thiamine.


Decarboxylation of pyruvate by thiamine analogues.


Studies on the biosynthesis of glucosamine in the intact rat.


The role of phosphorylation in glucose absorption from the intestine of the golden hamster.


beta-Hydroxybutyrate and acetate metabolism of the perfused bovine udder.


The biosynthesis of lycopene in tomato homogenates.


A sensitive and stereospecific enzymatic assay for xylulose.


Biosynthesis of glycolate and related compounds from ribose-1-C14 in tobacco leaves.


Studies on the metabolism of adipose tissue. I. The effect of insulin on glucose utilization as measured by the manometric determination of carbon dioxide output.


Propionic acid metabolism. II. Enzymatic synthesis of lactyl pantethine.


Studies on the metabolism of adipose tissue. II. The effect of changes in the ionic composition of the medium upon the response to insulin.


A method for incorporating cholesterol and other lipides into serum lipoproteins in vitro.


The effects of thyroxin administration on the enzymic reduction of delta 4-3-ketosteroids.


Electrophoretic behavior of serum amylase.


A simplified procedure for for the partial purification of acetylcholinesterase from electric tissue.


Preparation and microbiological properties of tripeptides of beta-2-thienylalanine.


Glutamic dehydrogenase. I. The effect of coenzyme on the sedimentation velocity and kinetic behavior.


Glutamic dehydrogenase. II. The effect of various nucleotides on the association-dissociation and kinetic properties.


The thyroxine-binding protein of bovine synovial fluid.


The inhibition of beef liver glutamic dehydrogenase by metal-binding agents.


Transamination reactions of mammalian cells in tissue culture.


xi-Aminocaproic acid: an inhibitor of plasminogen activation.


Properdin: preparation from plasma fraction I of the Cohn method.


The synthesis and some properties of amino acyl adenylates.


The enzymatic exchange of L-serine with O-phospho-L-serine catalyzed by a specific phosphatase.


Chromatographic evidence on the occurrence of thiotaurine in the urine of rats fed with cystine.


The metabolism of serotonin (5-hydroxytryptamine).


Accumulation of freely extractable glutamic acid by lactic acid bacteria.


The effect of vitamin B6 deficiency on glutamic acid accumulation by Lactobacillus arabinosus.


The utilization of glycine by the nucleated erythrocyte.


3-Hydroxyanthranilic acid metabolism. VII. Mechanism of formation of quinolinic acid.


The effect of norepinephrine on the 5-hydroxytryptophan decarboxylase activity of rat kidney.


Reaction of ethylene oxide with nicotinamide and nicotinic acid.


Reaction of ethylene oxide with histidine, methionine, and cysteine.


The biosynthesis on Ncarbamylhydroxyaspartic acid.


Inhibition of Saccharomyces cerevisiae by p-aminobenzoic acid and its reversal by the aromatic amino acids.


Enzymatic sulfurylation of tyrosine derivatives.


The effect of riboflavin deficiency upon the metabolism of tryptophan by liver and kidney tissue.


The source and state of the hydroxylysine of collagen. II. Failure of free hydroxylysine to serve as a source of the hydroxylysine or lysine of collagen.


The distribution of glutamic-gamma-aminobutric transaminase in the nervous system of the rhesus monkey.


The distribution of glutamic decarboxylase in the nervous system of the rhesus monkey.


Effect of hyperthyroidism on liver pyridine nucleotide synthesis.


Soluble gamma-aminobutyric-glutamic transaminase from Pseudomonas fluorescens.


Aldehyde oxidation. III. Succinic semialdehyde dehydrogenase.


Enzymatic utilization of acetylenic compounds. II. Acetylenemonocarboxylic acid hydrase.


Urinary metabolites of coumarin and omicron-coumaric acid.


The desulfuration of thiourea by thyroid cytoplasmic particulate fractions.


A bacterial pterin deaminase.

A bacterial pterin deaminase.


The respiratory chain in phosphorylating subfragments of mitochondria prepared with digitonin.


Evidence concerning the mechanism of adenosine triphosphate formation by spinach chloroplasts.


Hydrogen oxidation by Clostridium kluyveri.


Pyridine nucleotide transhydrogenase. VII. Determination of the reactions with coenzyme analogues in mammalian tissues.


Some effects of phlorizin on the metabolism of mitochondria.


Effect of phlorizin on the osmotic behavior of mitochondria in isotonic sucrose.


Phlorizin inhibition of the insulin expansion of the galactose space in the eviscerate rat.


Studies of the mechanism of action of the condensing enzyme.


The mechanism of the triosephosphate isomerase reaction.


Studies on the mechanism of the citrate condensing enzyme reaction.


Enzymatic reactions of fluoroacetyl phosphate.


Regulatory mechanisms in carbohydrate metabolism. I. Crabtree effect in reconstructed systems.


Regulatory mechanisms in carbohydrate metabolism. II. Pasteur effect in reconstructed systems.


Regulatory mechanisms in carbohydrate metabolism. III. Limiting factors in glycolysis of ascites tumor cells.


Regulatory mechanisms in carbohydrate metabolism. IV. Pasteur effect and Crabtree effect in ascites tumor cells.


The carbohydrate nutrition and metabolism of a strain of mammalian cells (MB III strain of mouse lymphoblasts) growing in vitro.


The effect of fructose feeding on glycolytic enzyme activities of the normal rat liver.


Biochemical changes in carbon tetrachloride fatty liver: separation of fatty changes from mitochondrial degeneration.


The enzymatic oxidation of 1,2-propanediol phosphate to acetol phosphate.


The absorption of fatty acids by the isolated intestine.


The accumulation of phosphate esters of mono- and dimethyl-ethanolamine by a mutant strain of Neurospora crassa.


The biosynthesis of beta-hydroxy-beta-methylglutaryl coenzyme A in yeast. I. Identification and purification of the hydroxymethylglutaryl coenzymecondensing enzyme.


The biosynthesis of beta-hydroxy-beta-methylglutaryl coenzyme A in yeast. II. The formation of hydroxymethylglutaryl coenzyme A via the condensation of acetyl coenzyme A and acetoacetyl coenzyme A.


The incorporation of mevalonic acid into tomato carotenoids.


Synthesis in vitro of steroids by human fetal adrenal gland slices.


Steroid-protein conjugates. II. Preparation and characterization of conjugates of bovine serum albumin with progesterone, deoxycorticosterone, and estrone.


The origin of cholesterol in liver, small intestine, adrenal gland, and testis of the rat: dietary versus endogenous contributions.


A pancreatic proesterase.

A pancreatic proesterase.


Mechanism of hydrolysis of adenosinetriphosphate by muscle proteins and its relation to muscular contraction.


Amino acid composition and amino-terminal sequence of yeast enolase.


The isolation and characterization of ribonucleases from sheep pancreas.


A comparative study of the structures of bovine and ovine pancreatic ribonucleases.


Trypsin inhibitors of human serum. II. Isolation of the alpha 1-inhibitor and its partial characterization.


Purification and metabolic effects of a nerve growth-promoting protein from snake venom.


Specificity of phosphoglyceric acid mutase.


Lactic dehydrogenase. V. Inhibition by oxamate and by oxalate.


Lactic dehydrogenase. VI. Fluorimetric measurements of the complex of enzyme and reduced diphosphopyridine nucleotide.


Lactic dehydrogenase. VII. Fluorescence spectra of ternary complexes of lactic dehydrogenase, reduced diphosphopyridine nucleotide, and carboxylic acids.


The nature and mechanism of the tryptophan pyrrolase (peroxidase-oxidase) reaction of Pseudomonas and of rat liver.


Isolation and identification of the products of the oxidation of choline.


Isolation and crystallization of gastricsin from human gastric juice.


Glutamic aspartic transaminase. II. The influence of pH on absorption spectrum and enzymatic activity.


1-Methylhistidine excretion by vitamin E-deficient rabbits.


Variation of glutamate metabolism with the site of injection.


Beef liver 3-hydroxyanthranilic acid oxidase.


Riboflavin and hepatic kynurenine hydroxylase.


Transmethylation in the biosynthesis of ergothionelne.


The role of vitamin B12 in thymine biosynthesis by chick bone marrow.


Conversion of nucleoside to sedoheptulose monophosphate by rat liver.


Studies on polynucleotides synthesized by polynucleotide phosphorylase. IV. P32-labeled ribonucleic acid.


The properties of adenosine deaminase and adenosine nucleoside phosphorylase in extracts of Escherichia coli.


Chemical properties of 3-substituted pyridine analogues of diphosphopyridine nucleotide.


Enzymatic studies with analogues of diphosphopyridine nucleotide.


The nucleotides in normal human blood.

The nucleotides in normal human blood.


Amino acid dependent exchange between pyrophosphate and adenosine triphosphate in spinach preparations.


Polyphosphate nucleosides of purine analogues.


The biosynthesis of deoxyribonucleic acid by the chick embryo. I. Utilization of cytidine-C14.


Studies on nucleic acid biosynthesis in Ehrlich ascites cells suspended in a medium permitting growth.


Studies on fluorinated pyrimidines. VIII. Further biochemical and metabolic investigations.


Concerning the specificity of streptococcal deoxyribonuclease (streptodornase).


Carbon dioxide fixation in marine invertebrates. I. The main pathway in the oyster.


Imidazole catalysis. II. Acyl transfer and the reactions of acetyl imidazole with water and oxygen anions.


Imidazole catalysis. III. General base catalysis and the reactions of acetyl imidazole with thiols and amines.


The binding of diphosphopyridine nucleotide by yeast glyceraldehyde-3-phosphate dehydrogenase.


The binding of analogues of diphosphopyridine nucleotide by yeast glyceraldehyde-3-phosphate dehydrogenase.


Catalysis of oxidation of nitrogen compounds by flavin coenzymes in the presence of light.


The effect of ribonuclease on oxidative phosphorylation by mitochondria.


Glycolytic and oxidative phosphorylative studies with intact and disrupted rat brain mitochondria.


Studies of reduced diphosphopyridine nucleotide oxidase: effects of isooctane extraction on enzymatic catalysis.


Interactions of catalytic cofactors for photosynthetic phosphorylation with Hill reaction oxidants.


The syntbesis of heparin by slices of mouse mast cell tumor.


The isolation of heparin from mouse mast cell tumor.


The purification and properties of muscle diphosphoglycerate mutase.


Distribution of two types of phosphoglyceric acid mutase, diphosphoglycerate mutase, and D-2,3-diphosphoglyceric acid.


Oxaloacetic decarboxylase from rat liver mitochondria.


Some observations concerning hepatic glucose 6-phosphate content in normal and diabetic rats.


Studies on the operation of the tricarboxylic acid cycle in scurvy.


Metabolic adaptations in higher animals. V. The study of metabolic pathways by means of metabolic adaptations.


The biochemical basis of phagocytosis. I. Metabolic changes during the ingestion of particles by polymorphonuclear leukocytes.


The metabolism of the organic acids of tobacco leaves. XVI. Effect of culture of excised leaves in solutions of malonate.


Biosynthesis of ribose and deoxyribose in Escherichia coli.


Serum phospholipide analysis by chromatography and infrared spectrophotometry.


The synthesis of phosphatidic acid from diglyceride and adenosine triphosphate in extracts of brain microsomes.


The mechanism of phosphate exchange in phosphatidic acid in response to acetylcholine.


Isolation of lipopolysaccharide endotoxin.


Metabolism of propionic acid in animal tissues. V. Purification and properties of propionyl carboxylase.


Separation of fatty acids from tubercle bacillus by gas chromatography: identification of oleic acid.


Incorporation of the butyryl unit of butyryl coenzyme A into palmitic acid by the pigeon liver system.


Metabolism of essential fatty acids. VIII. Origin of 5,8,11-eicosatrienoic acid in the fat-deficient rat.


The fractionation of cholesterol esters by silicic acid chromatography.


Influence of adenosine 3',5'-monophosphate on corticoid production by rat adrenal glands.


On the mechanism of squaiene biogenesis from mevalonic acid.


Tissue sources of human serum alkaline phosphatase, as determined by immunochemical procedures.


Isolation of gamma-guanidinobutyric acid from calf brain.


Ultracentrifugal studies on ribonucleoprotein from rat liver microsomes.


Physical and chemical studies of a limited reaction of iodine with proteins.


Properties and biotin content of purified preparations of the ornithinecitrulline enzyme of Streptococcus lactis.


Differences in mammalian amylases demonstrated by enzyme inhibition with specific antisera.


The preparation of subtilisn-modified ribonuclease and the separation of the peptide and protein components.


Passage of radioactive amino acids through nonprotein fractions of hen oviduct during incorporation into protein.


An enzyme that synthesizes cystathionine and deaminates L-serine.


The enzymatic reduction of hydroxyguanidine.


Metabolism of 2,6-diaminopurine: S-adenosylmethionine as methyl donor for 2-methylamino-6-aminopurine synthesis.


The regulation of activity of phosphoribosyl-pyrophosphate amidotransferase by purine ribonacleotides: a potential feedback control of purine biosyntoesis.


Inhibition of ribonucleic acid degradation in bacteria by spermine.


Virus-induced acquisition of metabolic function. I. Enzymatic formation of 5-hydroxymethyldeoxycytidylate.


Virus-induced acquisition of metabolic function. II. Studies on the origin of the deoxycytidylate hydroxymethylase of bacteriophage-infected E. coli.


Some factors which affect the enzymatic digestion of ribonucleic acid.


The influence of urea and electrolytes upon yeast ribonucleic acid.


Accumulation of uridine and cytidine nucleotides in Staphylococcus aureus inhibited by gentian violet.


Trace nucleotides in certain ribonucleic acids from yeast.


Iodide peroxidase in rat thyroid and salivary glands and its inhibition by antithyroid compounds.


Further studies on the mecnanism of action of carbamyl phosphate synthetase.


Purification and properties of the formate-activating enzyme from Micrococcus aerogenes.


The rate of ethanol metabolism in fed and fasting animals.


Kinetics of hydrolysis of the orthophosphate monoesters of phenol, p-nitrophenol, and glycerol by human prostatic acid phosphatase.


Fluorescence studies of coenzyme-binding to beef heart lactic dehydrogenase.


Acid-liked functions of intermediates in oxidative phosphorylation. I. Chemical equations.


Acid-linked functions of intermediates in oxidative phosphorylation. II. Experimental studies of the effect of pH upon respiratory, phosphorylative and transfer activities of liver and heart mitochondria.


Reactions of Rhodospirillum rubum extract with cytochrome c and cytochrome c2.


Respiration and light-induced phosphorylation in extracts of Rhodospirillum rubrum.


Stability of oxidative phosphorylation and related reactions in isolated liver mitochondria.


Photochemical determinations of the oxidases of bacteria.


The oxidation of omicron-aminophenols by cytochrome c and cytochrome oxidase. II. Synthesis and identification of oxidation products.


The oxidation of o-aminophenols by cytochrome c and cytochrome oxidase. I. Enzymatic oxidations and binding of oxidation products to bovine serum albumin.


Studies on choline dehydrogenase. I. Extraction in soluble form, assay, and some properties of the enzyme.


The oxidation of tyramine, tyrosine, and related compounds by peroxidase.


Biochemical studies of the developing avian embryo. III. The oxidation of reduced pyridine nucleotide.


Respiration and active sodium transport of isolated toad bladder.


A critical appraisal of photomicrographic illustration.


Negative identification.

Negative identification.


System of filing for medical photography.


Dark field illuminator for Petri dish photography.


Applications of autoradiography in biological research.


Film characteristic curves using the densichron and gray scale chips supplied with the densichron reflection unit.


Pre-print preparation of 16mm motion pictures.


Nonmedical photography in a medical institution.


The accumulation of soluble deoxyribosidic compounds in relation to nuclear division in anthers of Lilium longiflorum. Intervals of sporogenous DNA synthesis in lily anthers are associated with marked accumulations of methanol- and acid-soluble deoxyribosidic compounds. There are differences in solubility between compounds accumulating at meiosis and those accumulating at mitosis. The formation of these compounds appears to be related to a breakdown of somatic DNA.


Rapid cytochemical identification of phagosomes in various tissues of the rat and their differentiation from mitochondria by the peroxidase method. 1. Granules characterized by their ability to segregate foreign proteins (phagosomes) were identified in the cells of many rat organs after intravenous administration of horseradish peroxidase, by using the conventional test with benzidine for the histochemical detection of peroxidase. The largest numbers of phagosomes were identified in kidney and liver. Considerable numbers were observed cytochemically in pancreas, prostate, epididymis, thymus, spleen, bone marrow, small intestine, heart, pituitary, and mouse mammary carcinoma. 2. The variation in size of the phagosomes ranging from the limit of microscopic visibility up to 5 micro diameter, previously described for kidney, was also observed to occur in many of the other organs. The average size of the phagosomes in different organs was also different, the phagosomes of the liver, for example being on the average smaller than those of the kidney, pancreas, and prostate. 3. In squash preparations of kidney and liver, the phagosomes appeared often in curved rows following the course of the cell membranes of epithelial cells. In several other organs, they appeared aggregated in cells located in the vicinity of blood or lymphatic vessels or capillaries. 4. After injection of peroxidase directly into the brain of a rabbit, a striking concentration of peroxidase was observed in phagosomes of endothelial cells of capillaries and vessels, surrounding the site of injection. It was suggested that this localization may offer an explanation for the so called blood-brain barrier. 5. The cytochemical peroxidase method was applied to smears of isolated fractions of kidney and liver. Only the isolated phagosomes, but not the isolated nuclei, mitochondria, and microsomes, reacted with benzidine after administration of peroxidase. The contamination of conventionally prepared nuclear, mitochondrial, and microsomal fractions of kidney and liver with phagosomes of different sizes was observed. By correlating the cytochemical peroxidase test of smears of isolated fractions with the colorimetric determination of peroxidase, acid phosphatase, and cytochrome oxidase in the same fractions, the differentiation of the phagosomes from mitochondria and other cell granules was facilitated. 6. The marked difference in the osmotic properties of phagosomes and mitochondria, detectable after treatment with 70 per cent alcohol, and the difference in their affinities towards basic fuchsin, made it possible to differentiate the phagosomes from the mitochondria. It was found by this simple procedure that kidney cells of normal rats contain a large number of phagosomes ranging in size from 0.5 to 3 micro, whereas liver cells of normal rats contain relatively few phagosomes of this size but many smaller ones (0.2 to 0.5 micro diameter). These increased in size after treatment of the rats with horseradish peroxidase.


Ultraviolet radiation effects on Ehrlich ascites tumor cells; observations using a flying spot ultraviolet microscope. A flying spot ultraviolet microscope, employing a fast scan and pulsed operation of the raster, has been used to induce radiation damage in ascites tumor slide cultures, and to study by time-lapse cinematography the progressive stages of cell damage. The cells observed came from a strain (EF(7)) of the Ehrlich ascites carcinoma. Irradiated cells were found to show a characteristic syndrome of damage, involving blebbing at the cell surface, while control cells in the adjacent areas of the preparation remained unchanged. The end of the blebbing period is marked by swelling of the cells, and the time taken for this phenomenon to occur was used as a measure of the severity of the damage. It was found that the time required for swelling is dependent on the size of the dose employed, as well as on the sensitivity of the cells. This latter sensitivity was found to decline as the physiological age of the tumor increased. If ultraviolet illumination below 255 mmicro is excluded, no symptoms of damage occur, even when very large doses are used. These observations are discussed in relation to the nature of the system in the cell which is affected.


A cytochemical study of the stem cell concept in specimens of a human ovarian tumor. The amounts of DNA in interphase nuclei were compared with the amounts of DNA in metaphase and anaphase figures in Feulgen-stained tissue sections of 5 specimens of the human ovarian papillary serous adenocarcinoma. The relative amounts of DNA per cell were determined by cytophotometric measurements of interphase nuclei at a single wavelength and of mitotic figures by the two wavelength method. The 5 specimens conformed to the stem cell concept of cell proliferation since anaphase distributions of amounts of DNA were restricted to a narrow range of DNA values indicating the successful mitosis of a single cell type (stem cell) out of several cell types whose presence were suggested by the wide spread of interphase and metaphase values. In addition, the data indicated that, in some instances, only the amounts of DNA in anaphase figures can reliably identify the stem cell. Changes in the frequency of dividing cells having doubled amounts of DNA, and/or the presence of cells resulting from endoreduplication can distort the interphase distribution of amounts of DNA and thus give rise to a modal DNA interphase value which is not the same as the DNA value of the stem cell (anaphase figures).


Cytological evidence that both RNA and DNA may form a complex with the same protein. Deoxyribonucleic acid can be added back to protein sites from which the original nucleic acid, ribo- or deoxyribo-, is removed. If sections of frozen-substituted ovarian follicle cells of a leafhopper are first extracted by hot trichloracetic acid to remove nucleic acids and then immersed in a solution of a commerical preparation of deoxyribonucleic acid, the nucleic acid becomes attached to nuclear and cytoplasmic sites and can be rendered visible by the Feulgen reaction. The addition occurs in certain other tissues as well. The results are discussed in relation to biochemical and other cytochemical investigations of the nucleoprotein complex.


Functional significance of the crystal cells in the larva of Drosophila melanogaster. Cytoplasmic crystalline inclusions are found in some larval haemocytes of Drosophila melanogaster. Blackening can be experimentally induced in these cells, and previously it was suggested that either the substrate or enzyme for the tyrosine-tyrosinase system leading to melanin production in Drosophila larvae is found in these inclusions in the crystal cells. The present report is an attempt to further localize the enzyme and substrate. Larvae have been fed on food containing alpha-C(14)-tyrosine and autoradiographs of the blood cells taken from these larvae subsequently prepared. The C(14) activity in the crystal cells is restricted to the crystal inclusions in the cells and is significantly higher than that found in the other type of haemocytes, the plasmatocytes. When samples of the blood cells are incubated in DOPA solution, the extra-crystalline cytoplasm becomes blackened while the crystals themselves remain colorless. These observations are consistent with the hypothesis that the substrate is localized in the crystal inclusions whereas enzyme is found in the surrounding cytoplasm. An in vivo structural isolation would serve to separate enzyme and substrate rather than an inhibition by dehydrogenases as postulated by previous authors. In vitro examination with the phase microscope has shown that the crystal cells rupture easily and the crystals dissolve in the haemolymph. Therefore any treatment which tends to disrupt the structural integrity of the cell will allow the enzyme and substrate to come together. Humoral factors preceding metamorphosis might account for the in vivo release of the enzymatic reaction by initially altering the permeability of the cell.


The fine structure of a multiterminal innervation of an insect muscle. The detailed structure of nerve branches, neuromuscular junctions, and muscle fibers of a multiterminal innervation of cockroach abdominal muscle has been studied with the electron microscope. The muscle fiber is of the banded myofibril type; with paired mitochondria and abundant endoplasmic reticulum. The peripheral nerve branches are multiaxonal with large central axon and several small peripheral tunicated axons. Tracheoblasts closely accompany the nerve branches. The multiple neuromuscular junctions show typical axonal vesicles, muscle aposynaptic granules, and close plasma membrane apposition with no interposition of basement membrane material.


Submicroscopic changes in visual cells of the rabbit induced by iodoacetate. Alterations produced by iodoacetate in visual cells have been studied under the electron microscope. Lesions of the outer segments of the rods are visible as early as 3 hours after a single injection of 20 mg. iodoacetate per kg. body weight. After 6 hours the changes are more marked and consist then of disorganization, vesiculation, and lysis of the rod sacs. The inner segments of most rod cells show swelling and vacuolization of the matrix, the endoplasmic reticulum, and the Golgi complex. The mitochondria of the ellipsoid show a tendency to disintegrate. In some inner segments the changes consist primarily in an increase in density of the matrix and deposition of a granular material. The rod synapses are also affected, showing lysis of the synaptic vesicles and alterations of the synaptic membrane. With a second injection of 20 mg. iodoacetate per kg. body weight, all these changes become more marked and lead to complete destruction of the rod cells. The cones seem more resistant than the rods. A single injection produces no visible changes in the outer or inner segments of the cones. At cone synapses, however, there are changes consisting of fusion of synaptic vesicles and other membranous material to form large concentric membranes characteristic of myelin figures. A second dose of the drug causes complete destruction of the cone cells. All these, and other submicroscopic changes, are discussed in relation to various hypotheses put forward to explain the mode of action of iodoacetate on visual cells. The pronounced alterations of submicroscopic intracellular membranes suggest that the locus of action of iodoacetate may be a component widely dispersed throughout the visual cells and related, in some way, to the maintenance of these lipoprotein structures.


The electron microscopy of the left colleterial gland of the cockroach. A study has been made of the cells of the left colleterial gland of the cockroach, Periplaneta americana (L.), using the electron microscope, and the results compared with previous histological and histochemical studies. The colleterial gland consists of an arborescent bunch of long tubules composed mainly of the cells which secrete the structural protein of the egg case ("type 4 cells"). Other types of cells: chitinogenic cells and "type 2 and 3 cells" each with a different cytology are described. The type 4 cells, which form the structural protein, reveal a cytological pattern very similar to that described for mammalian cells in a state of active protein synthesis. There is an elaborate development of particle-studded membranes in the cytoplasm. Smaller, rounded agranular vesicles also occur. The free secretory surface of the secreting cells forms the "end-apparatus" of the light microscopists. The invaginated surface is cast into numerous long narrow processes usually radially arranged and directed into a funnel-like formation derived from the thin intima lining the lumen of the gland (Text-fig. 2). The secretion in the form of small balls may be seen in the cavity of the end-apparatus and sometimes in the narrow processes. The small chitinogenic cells, lying between the protein-forming cells and the thin intima which they secrete, have a different cytology perhaps related to the fact that they form a polysaccharide rather than a protein. There is a very poor development of the particle-studded membranes of the type found in protein-forming cells. The type 2 cells, supposed to form an oxidase, have an end-apparatus that is similar to, but more complex than, those of the type 4 cells and their cytoplasm is almost completely filled with mitochondria. There is some evidence that mitochondria play a part in forming the oxidase and pass into the tubules of the end-apparatus. Type 3 cells resemble both types 2 and 4 and are probably a transient intermediate form.


Cytophotometric study of deoxyribonucleic acid in cortisone-treated rat hepatocytes. Rats were treated by intramuscular injection with cortisone acetate, 25 mg./day for 5 days. Small pieces of liver obtained from treated and normal animals were squashed on a microscope slide so as to obtain many areas only a single cell in thickness. After Feulgen staining to demonstrate DNA, optical density was measured using a projection technique. In both the normal and treated animals the nuclei were easily segregated in three ploidy classes, diploid, tetraploid, and octaploid, depending upon Feulgen intensity. In all three classes, the absorbence of nuclei from cortisone-treated animals was approximately 20 per cent lower than the normal. These data were interpreted to indicate that a change in DNA content had been induced by cortisone administration. These findings are comparable to data obtained from similar animals using chemical methods for the determination of DNA.


Electron microscope observations on in vitro cultures of the isolated fowl embryo otocyst. In vitro cultures of isolated fowl embryo otocysts were studied with the electron microscope. Hair cells of the developing organ of Corti and crista ampullaris have been examined with particular reference to the structure of the cilia and of the cell membrane. Two types of hair cells could be distinguished on the basis whether or not they possessed a "kinocilium" and "stereocilia," or "stereocilia" only. The cytoplasmic membranes were simple and there were no multiple vesicular layers in any of the hair cells. The supporting elements consisted of supporting cells flanking the hair cells, fibroblasts, and the cartilaginous otic capsule. Both the cochlear and vestibular sensory area showed rich innervation by mainly non-myelinated fibers with partial myelinization in others. There were well developed ganglion cells present. Bare axons penetrated the basement membrane and spread, amongst the supporting cells sheltering them, to the base of the hair cells where they formed bud-shaped nerve endings but, at the stage of development examined, no calyces. These in vitro cultures of the isolated fowl embryo otocyst provided convenient and suitable material for the electron microscope study of the sensory epithelium of the ear and revealed further that the isolated fowl embryo otocyst possesses great powers of self-differentiation also at the ultrastructural level.


Electron microscopy of the sperm tail; results obtained with a new fixative. The details of a new fixation procedure using 40 per cent osmium tetroxide in carbon tetrachloride are presented. This fixative is a good general preservative, gives a higher contrast than the ordinary osmium fixatives, and may also preserve structures that are not otherwise readily revealed. Some possible reasons for the increased contrast are discussed. Micrographs of the sea urchin spermatozoa treated with the new fixative provide more detailed information on the tail structure than has heretofore been obtainable. This information is summarized in the diagrammatic text-figure. The sperm tail can no longer be regarded as having a bilateral symmetry, and thus, it is possible to assign an index number to each of the nine peripheral filaments. The nine peripheral filaments have a complex morphology, each one of them seems to be composed of two subunits that have unequal diameters. The slightly larger subunits are all found in the clockwise direction with regard to the other subunit or are all found in the counter-clockwise direction in the sectioned tail. Each of the slightly larger subunits is at intervals provided with two types of projections-referred to as the arms and the spokes-that extend in respective tangential and radial direction. The arms from one filament may be in actual contact with its neighboring filament through a complex bridge-like formation. There is a quantitative difference between the nine filaments with regard to this bridge. It is assumed that the eleven tail filaments follow straight paths. Some hypotheses on sperm movement are discussed based on this assumption and on the fact that the oscillations of an actively working sperm tail are in one plane. Probably, the nine peripheral filaments have non-equivalent functions in tail movement. In the centriole the nine peripheral filaments characteristically appear as triplets in a whorl-like arrangement. It is suggested that the inner part of this triplet is a derivation of the arms. A structural abnormality of the tail is described that is characterized by two or three complete sets of tail filaments within one cell membrane.


Further studies on the lyo and desmo components of several hydrolytic enzymes and their histochemical significance. This report describes additional studies of the lyo and desmo components of esterase, alkaline phosphatase, acid phosphatase, leucine aminopeptidase, and beta-glucuronidase. The techniques used have already been reported (7). Enzyme diffusion occurs to different degrees in different fixatives, and varies somewhat with each enzyme. Loss of enzymatic activity during fixation occurs as a result of both inactivation due to the chemical reaction of the fixative with the enzymic protein, and diffusion of the lyo component into the fixative. The amount of diffusion into formalin can be reduced by the addition of salts, sucrose, or methocel. The pH of the aqueous medium significantly influences the removal of the lyo fraction from the tissue section. A striking similarity can be noted in the proportions of each fraction of enzyme present in the kidney of the rat, dog, and man. The procedure of fixation and paraffin embedding of tissue blocks does not wholly prevent the diffusion of the lyo component from the tissue sections when they are subsequently immersed in the aqueous incubation medium.


Observations on cellular structures of Porphyridium cruentum. The cellular structure of Porphyridium cruentum was studied with both light and electron microscope. The photosynthetic plastid in this red alga was found to be structurally similar to that in the Chlorophyceae and higher green plants. The phycobilins, as well as the chlorophyll, seem to be associated with the lamellae of the plastid. The pyrenoid, a region of low lamellar density, contains no tubules, and does not appear to function in synthesis or storage of reserve material. Grains of floridean starch are located in the cytoplasm, outside the plastid. Typical mitochondrial organelles were not observed. The nucleus is eccentric, and contains a nucleolus located on the inner face of the nucleus, nearest the plastid. The schedule for staining the nucleus is given in detail. Other cell structures (sheath, dictyosomes, etc.) are described. Growing cells in light of intensity leads to disruption of the parallel arrangement of the lamellar characteristic of cells grown in moderate light.


Macronuclear duplication in the ciliated protozoan Euplotes. The ribbon-like macronucleus of Euplotes eurystomus pinches in half amitotically at each cell division. Several hours before the actual division two lightly staining duplication bands (reorganization bands) appear at the ends of the nucleus and approach each other slowly, finally meeting near the middle. Distal to the bands, that is, in regions through which the bands have already passed, the concentration of DNA (Feulgen) and "histone" (alkaline fast green) is greater than in the central zone. These facts suggest the hypothesis that DNA-histone synthesis takes place in a sequential fashion starting at the tips of the nucleus and proceeding to the middle. That this hypothesis is correct is shown by autoradiographic and photometric observations. Tritium-labelled thymidine is incorporated only in a limited region immediately distal to the bands. The average amount of Feulgen dye bound by the nucleus rises as the duplication bands approach each other, and is double the presynthesis value by the time the bands meet. A similar rise in the alkaline fast green dye is seen in duplicating nuclei, although no completely post-synthesis values were obtained in this study. The quantitative data are consistent with the assumption that the macronucleus contains a number of DNA-histone "units," presumably chromosomes, each of which duplicates once and only once.


Factors influencing the histochemical demonstration of coenzyme-dependent dehydrogenases and diaphorases. Procedures for the histochemical demonstration of DPN and TPN diaphorases have been presented by other workers. These techniques rely on the coenzyme-dependent dehydrogenases present in the tissue slice to generate the substrate required by the diaphorases. In vitro studies were carried out on kidney and adrenal tissue of the rat, using NT (neotetrazolium) and INT (2-p-iodophenyl-3-p-nitrophenyl-5-phenyl tetrazolium chloride) with various substrates of DPN-dependent dehydrogenases. The solutions used for study contained alcohol and alcohol dehydrogenase, glutamate and malate, malate, glutamate, beta-hydroxybutyrate, or DPNH. It has been possible to demonstrate (1) that histological distribution of dehydrogenases may differ from that of the flavoprotein oxidizing reduced coenzyme I; (2) characteristic patterns of distribution of particular dehydrogenases in the tissue proper; (3) different levels of dehydrogenase in kidney and adrenal; and (4) differences in dehydrogenase distribution in the kidneys of man and rat. The evidence presented clearly indicates the limitations inherent in the accepted procedures for the demonstration of DPN and TPN diaphorases. The possible application of the tetrazolium salts to the study of particular coenzyme-dependent dehydrogenases and the pitfalls which might occur are also discussed.


C14 assays and autoradiographic studies on the rooster comb. The distribution of C(14) was studied in various parts of the rooster comb following treatment with testosterone. The value of gas-phase assay of C(14) in tissue has been demonstrated and the results compared with those of autoradiographic studies on the same tissue. The results of these experiments showed that androgen treatment significantly increases the rate of incorporation of C(14) in various parts of the comb. The specific activity of carbon in the comb, cornea, and liver differed, depending on which precursor, viz. glucose-6-C(14), glucose-1-C(14), and glucuronolactone-U-C(14), was administered. The highest values were obtained after the administration of glucose-6-C(14); glucuronolactone-U-C(14) gave the lowest specific activity. The specific activity of carbon in different parts of the comb showed considerable variation. Carbon assay of serial sections of the comb cut at various planes showed that the specific activity of carbon was highest in the mucoid layer. Both C(14) assays and autoradiograms indicate that C(14) is also present in other parts of the comb. As seen in autoradiography, the concentration of C(14) was highest in the epithelium, in the blood vessel walls, and in the avascular collagenous tissue. These results, and indications from previous studies, suggest that the high specific activity of carbon in the mucoid layer is due mainly to the presence of C(14)-labelled hyaluronic acid. Autoradiograms and PAS staining suggest that a significant amount of C(14) is also incorporated into the glycoproteins associated with the collagen fibers.


An electron microscope study of the rat ovum. This paper reports on the fine structure of rat oocytes at stages before ovulation, during maturation, fertilization, and early cleavage. The study includes parallel observations on light and electron microscope preparations with attempted correlations. The follicular cells of the ovarian egg are described as sending long processes through the zona pellucida to the egg surface where they mingle with thin projections from the egg itself. No open communication between follicle cell cytoplasm and egg cytoplasm was observed. During maturation and fertilization both types of processes are withdrawn from the zona. The germinal vesicle and later the pronuclei of the fertilized egg are characterized by numerous large nucleoli. These have the form of thick walled vesicles with diameters as great as 8 to 10 micro. The wall is dense in the EM image and appears to consist in part of small granules. The cytoplasm shows several inclusions including mitochondria of usual form and a Golgi component which has the typical fine structure and the distribution described by earlier light studies. Small dense particles, presumably RNP particles, are distributed throughout the cytoplasmic matrix and show no preference for membranes. The endoplasmic reticulum of the oocyte is represented by a scattering only of vesicles, but begins a more extensive and elaborate development with the onset of segmentation. One inclusion of the ooplasm, similar in size to mitochondria, receives special attention. It is a vesicular structure, containing a large number of small vesicles (10 to 50 mmicro in diameter) and frequently a central density or nucleoid. They are referred to as multivesicular bodies. Such bodies are found in small number in the ovarian egg, but increase greatly in number during maturation and fertilization. It appears from the micrographs of eggs in these latter stages that these vesicular bodies break down and liberate their content of small vesicles to the surrounding ooplasm. Comments are provided on the apparent significance of the various observations.


The use of mercuric bromphenol blue as a stain for electron microscopy.


Relationships of the ovular surface with follicle cells and origin of the zona pellucida in rabbit oocytes.


On the radiomimetic effects of cupferron and potassium cyanide.


Some immunochemical studies on the mitotic apparatus of the sea urchin.


The total lung capacity and its subdivisions in respiratory poliomyelitis.


Statistical associations between international coronary heart disease death rates and certain environmental factors.


The effect of chlorambucil (CB 1348) on growth and metabolism.


Adjustment to disability; some observations on patients with multiple sclerosis.


Survey of 230 medical patients in the Baltimore City Chronic Disease Hospital.


Urban reservoirs of tuberculosis.

Urban reservoirs of tuberculosis.


Distribution of stretch and twist along the growing zone of the sporangiophore of Phycomyces and the distribution of response to a periodic illumination program.


Further studies of 21-deoxyhydrocortisone-4-alpha C14 in man.


Pathways of terminal respiration in marine invertebrates. I. The respiratory system in Cephalopods.


The effects of nitrogen, helium, argon and sulfur hexafluoride on the development of insects.


Morphogenesis and metabolism of amphibian larvae after excision of heart. III. Effect of sodium azide on respiratory metabolism of heartless larvae of Rana pipiens.


Serum glutamic-oxalacetic transaminase and serum glutamic-pyruvic transaminase in toxemia of pregnancy.


Metabolic studies in pregnancy and the puerperium.


Urinary excretion of 17-ketosteroids following rectal infusion of cortisol.


Studies of the diurnal variation of plasma 17-hydroxycorticosteroids in man.


Swimbladder volume, buoyance, and behavior in the pinfish, Lagodon rhomboides (Linn.).


The influence of thyroid hormones on metabolism in psychiatric disorders. I. The effect of 3:5:3'-triiodothyronine on calcium and phosphorus metabolism in psychiatric patients.


Functional relationships between the liver, the thyroxine-binding protein of serum, and the thyroid.


Pseudo-pseudohypoparathyroidism with unusual features.


A euthyroid man without thyroxine-binding globulin.


The isolation of delta 4-pregnene-11 beta, 17 alpha, 20 alpha, 21-tetrol-3-one and delta 4-pregnene-11 beta,17 alpha,20 beta, 21-tetrol-3-one from human adrenal incubates.


Depression of the serum cholesterol level by triiodothyropropionic acid.


Utilization of fructose by the nervous system in man.


Flavin enzymes in liver and kidney of rats from birth to weaning.


Assay of human pituitary gonadotropin from male and postmenopausal urine.


A comparative study of the metabolism of hydrocortisone and prednisolone.


Urinary 17-hydroxycorticosteroids and 17-ketosteroids in castrated vs. intact men: supplementary comparisons in feeble-minded vs. mentally normal men.


The serum pattern of thyroid hormones in euthyroidism and hyperthyroidism.


The ten-minute uptake of I 131: a clinical study and comparison with other tests of thyroid function.


Treatment of malignant edematous exophthalmos by implantation of the pituitary with yttrium90: report of two cases.


The effects of varying amounts of stable iodide on the function of the human thyroid.


Idiopathic hypoparathyroidism: unusual response to vitamin D.


Effects of 2-methyl-dihydrotestosterone and of testosterone on human pituitary gonadotropin.


Urinary steroids in 10 cases of adrenal carcinoma, with special reference to pregnane-3 alpha, 17 alpha,20 alpha-triol-11-one.


Beta and delta cells of the human adenohypophysis: their response to adrenocortical disorders.


Daily variations in the thyroidal uptake of I 131 in human subjects.


Iodine metabolism in goitrous cretins.

Iodine metabolism in goitrous cretins.


A consideration of the hypercalciuria in sarcoidosis, idiopathic hypercalciuria, and that produced by vitamin D; a new suggestion regarding calcium metabolism.


The acute effects of parathyroid extract in states of edema, diminished renal function and parathyroid disease.


Effect of hypnoticallyinduced anxiety on the plasma hydrocortisone level of normal subjects.


Effect of prolonged sleep therapy and of chlorpromazine on plasma protein-bound iodine concentration and plasma thyroxine turnover.


Repository forms of adrenocorticotropin (ACTH).


Thyroid adenomas and thyrotoxicosis in patients with hypopituitarism following hypophysectomy.


Addison's disease in two brothers.

Addison's disease in two brothers.


Intravenous progesterone in the anovulatory woman.


The effect of thyrotropin on the turnover rates of thyroxine and triiodothyronine.


Serotonin metabolism in liver disease.

Serotonin metabolism in liver disease.


Calculation of intracellular pH from the distribution of 5,5-dimethyl-2,4-oxazolidinedione (DMO); application to skeletal muscle of the dog.


Effects of reduced glomerular filtration on urine concentration in the presence of antidiuretic hormone.


The role of urea in the urine concentrating mechanism.


Toxicity of urea and its role in the pathogenesis of uremia.


Sialic acid in human semen and in the male genital tract.


Transmission of the common cold to volunteers under controlled conditions. IV. Specific immunity to the common cold.


The relation of urinary CO2 tension to bicarbonate excretion.


A practical study of the air chamber model of the cardiovascular system.


The renal clearances of iodide and L-diiodotyrosine measured simultaneously.


The effect of triiodothyronine on the oxidative metabolism of erythrocytes. I. Cellular studies.


Pyrimidine metabolism in man. I. The biosynthesis of orotic acid.


Studies on enhanced fibrinolytic activity in man.


Studies in kernicterus. I. The protein binding of bilirubin.


Effect of altered intrathoracic pressure on renal hemodynamics, electrolyte excretion and water clearance.


The effects of adrenal steroids and potassium depletion on the elaboration of an osmotically concentrated urine.


Studies on a case of essential hyperlipemia: blood lipids, with special reference to the composition and metabolism of the serum glycerides before, during and after the course of a viral hepatitis.


Comparative studies on the half-life of I 131-labeled albumins and nonradioactive human serum albumin in a case of analbuminemia.


A biochemical study of certain skeletal muscle constituents in human progressive muscular dystrophy.


Combined anterior pituitary and neurohypophyseal insufficiency; studies of body fluid spaces and renal function.


Studies of the effect of dietary protein and caloric levels on the kinetics of nitrogen metabolism using N15 L-aspartic acid.


Tolbutamide hypoglycemia in acutely depancreatized dogs.


Studies of the uricogenic effect of 2-substituted thiadiazoles in man.


A study of the mechanism by which exercise increases the pulmonary diffusing capacity for carbon monoxide.


In vivo studies of 5-hydroxyindole metabolism in patients with hepatic cirrhosis and in rats.


The kinetics of bicarbonate reabsorption during acute respiratory acidosis.


Mucopolysaccharide content of skin in patients with pretibial myxedema.


Tissue and renal response to chronic respiratory acidosis.


The role of the monkey fetus in the exchange of the water and sodium of the amniotic fluid.


The role of the fetus in the water exchange of the amniotic fluid of normal and hydramniotic patients.


Observations on growth and development of clinical investigation.


Acidosis and alkalosis: a modern view.

Acidosis and alkalosis: a modern view.


Electrolyte excretion in respiratory failure.


Gastric proteolysis in disease. I. The proteolytic activity of gastric juice from patients with pernicious anaemia.


An evaluation of the euglobulin method for the determination of fibrinolysis.


Mercury in biological materials.

Mercury in biological materials.


Changes in buccal cells in the anaemias.


The epithelial cells in megaloblastic anaemias.


Oral cornification in anaemic patients.

Oral cornification in anaemic patients.


Observations on the aminoaciduria in megaloblastic anaemia.


Amino-acid tolerance curves and amino-aciduria in Cooley's and sicklecell anaemias.


Dilithium sequestrene as an anticoagulant.


The use of a sequestrene-citrate mixture in the estimation of the blood sedimentation rate.


The effect of sequestrene and heparin on the erythrocyte sedimentation rate.


Platelet serotonin and normal haemostasis.


The practical value of the frog test in the diagnosis of normal and pathological pregnancy.


Pulmonary haemorrhage with extensive glomerular disease of the kidney.


A renal lesion in association with influenza.


Staphylococcal cystitis in a gynaecological ward.


A study on neuroglia: the problem of transitional forms.


A survey of rhinencephalic interconnections with the brain stem.


Efferent pathways from the deep cerebellar nuclei of the cat.


Some projections from the peri-central cortex to the pons and lower brain stem in monkey and chimpanzee.


Forced horizontal conjugate gaze in man.


The development of the pelvic plexuses and the distribution of the pelvic splanchnic nerves in the human embryo and fetus.


Responses to electrical stimulation of the cerebellum of unanesthetized birds.


Hunger, deprivation, and the day-night cycle.


Emotional reactions of rats to the pain of others.


A test of a theory of shape discrimination in Octopus vulgaris Lamarck.


Visual movement and intensity discrimination in cats after early deprivation of pattern vision.


Palatability and osmotic pressure of glucose and sucrose solutions as determinants of intake.


Mass action and early environment in the rat.


The electrical response of the auditory mechanism in the bullfrog (Rana catesbeiana).


The parasympathetic component of unlearned and acquired cardiac responses.


Cerebral cholinesterase activity and hypothesis behavior in a problemless maze.


Effect of pentobarbital sodium on the behavior of rats in the Krech hypothesis apparatus.


Maze performance of mature rats recovered from early postnatal infection with Murray Valley encephalitis virus.


The anorexigenic action of dextro-amphetamine sulfate upon feeding responses of differing strength.


Effect of acquisition level upon the magnitude of stimulus generalization across sensory modality.


The effect of training procedure upon auditory frequency discrimination in the cat.


Discrimination and reversal learning as a function of change of sensory stimulation.


Learning as a function of sensory stimulation of various intensities.


Two determinants of the emergence of anticipatory avoidance.


The effect of a feeding procedure on persistent avoidance responses in rats.


Extinction of avoidance as a function of the escape situation.


Effect of adrenal demedullation on an avoidance response in the rat.


Successive visual discriminations in white rats.


The influence of reward on alternation behavior.


The formation of a temporal-sequence learning set by monkeys.


Oddity learning as a function of mental age.


Inferential learning in rats: the problem-solving assembly of behavior segments.


Conditioning in fish (Mollienisia sp.) as a function of the interval between CS and US.


Imprinting and level of anxiety.

Imprinting and level of anxiety.


Effects of situational anxiety on sexual behavior in male rats.


Effects of ejaculation on sexual behavior in the male rat.


Autoradiographs of solder joints with fluorine.


Dental conditions of preschool children (one to six years of age) in Szeged, Hungary.


The distribution of nitrogen in human enamel.


Distribution of some enzymes in soft tissues of the oral cavity.


Comparative effects of fluorides on mice femurs.


The relative local anesthetic potencies of procaine, oracaine, kincaine, and xylocaine.


Phase composition of crown and inlay porcelains.


Study of the setting of plaster.

Study of the setting of plaster.


Phosphatase activity in whole and parotid saliva and its relationship to dental caries.


Observations on the development of oxytalan fibers in the periodontium of man.


A source of error in alkalinity titrations of saliva.


The effect of peptides and amino acid derivatives on acid formation in sugar-saliva mixtures.


Dental caries in the Syrian bamster. VIII. The induction of rampant caries activity in albino and golden animals.


Decomposition of gypsum investment in the presence of carbon.


Sutural expansion by controlled mechanical stress in the rat.


The localization of the presumptive cerebral regions in the neural plate of the axoloti larva.


Studies in embryonic and larval development in Amphibia. I. The embryology Eleutherodactylus ricordil, with special reference to the spinal cord.


Electron microscopy of the amelodentinal junction during early development of the molars of hamsters.


Genetical and development studies on droopy-eared mice.


Protein accumulation in early chick embryos grown under different conditions of explantation.


Urinary fluorine levels in women during pregnancy and after delivery.


Histologic observations on the morphology of the blood vessels of canine and human tooth pulp.


The diffusion of glucose through microbial plaques.


The BW1081 mouse salivary gland tumor; a preliminary study of its growth characteristics and host responses.


Studies on salivariadenectomized Hunt-Hoppert caries-resistant rats. III. Effect of salivariadenectomy on dental caries and several groups of the oral microflora under controlled feeding conditions.


Studies of the mode of outgrowth of the amphibian pronephric duct.


The effect of varying peroxide and polymer on the rate and degree of polymerization of polymethyl methacrylate slurries.


The development of the nervous system in chick embryos, studied by electron microscopy.


Studies on tubercle bacillus-monocyte relationship. III. Conditions affecting the action of serum and cells; modification of bacilli in an immune system. Studies of the relationship of protective serum factor to cellular resistance and to tuberculin skin sensitivity have demonstrated that protective serum factor may exist independently of a high level of cellular resistance, and that both protective factor and cellular resistance may be demonstrable without a concomitant hypersensitivity of the delayed type. The experiments with absorbed sera and the globulin fraction of immune serum indicated no specific association of protective serum factor with antibody globulin. The protective factor in immune serum was found to be thermostable and non-dialyzable. In vitro exposure of virulent tubercle bacilli to the immune serum from BCG-immunized animals failed to alter the bacterial capacity for destruction of monocytes and for intracellular proliferation. In vitro cultivation of normal and immune monocytes in normal or immune serum was not effective in changing the native susceptibility or resistance of these cells. Effective manifestation of resistance to virulent tubercle bacilli by immune monocytes was found to require the continuous presence of immune serum. The intracellular passage of virulent tubercle bacilli in an immune system (immune monocytes cultivated in immune serum) resulted in a decreased bacterial potential for destruction of normal monocytes when these were cultivated in the presence of immune serum.


Studies on primary atypical pneumonia. II. Observations concerning the development and immunological characteristics of antibody in patients. By using the indirect method of fluorescent staining to study the antibody response in patients with primary atypical pneumonai associated with the development of cold agglutinin, it was found that the PAP antibody developed during the 2nd and 3rd week of the illness, and persisted for over a year, and is not related to the cold and streptococcus MG agglutinins. The development of the PAP fluorescent staining antibody paralleled the neutralizing antibody for the PAP virus as tested in cotton rats. The sensitivity of this specific serological test was indicated by the observation that 67 to 92 per cent of the patients in several outbreaks of PAP showed a rise of antibody titer during convalescence. Absorption of the sera with various tissue powders did not affect the PAP antibody detected by this method.


Studies on the formation of collagen. IV. Effect of vitamin C deficiency on the neutral salt-extractible collagen of skin. The skin of severely scorbutic guinea pigs which were losing weight contained no detectible neutral salt-extractible collagen. Conditions of growth (weight gain) which actively induced the formation of neutral salt-extractible collagen in the skin of normal guinea pigs failed to do so in the animal with ascorbic acid deficiency. No excess of non-collagenous proline was found in neutral salt extracts of scorbutic skin as compared with normal. Fractionation of these extracts failed to reveal the presence of significant amounts of a soluble component containing unusual proportions of glycine and proline relative to hydroxyproline. It is concluded that deficiency of ascorbic acid either interferes with the synthesis of new collagen in intact skin or causes its destruction and removal as rapidly as it is produced.


Parathyroid transplants in rats; a comparison of their survival time with that of skin grafts. 1. A reliable method of parathyroid grafting in rats, and of assessing the functional state of such grafts in terms of blood calcium has been devised. 2. Parathyroid homografts between rats of proven genetic diversity may be rejected as promptly as skin; but the majority survive for an indefinite period, there being no intermediate class. 3. Surviving parathyroid grafts in this genetically diverse combination invariably succumb in the presence of the immunity induced by a later skin graft from the same donor. 4. Parathyroid grafts between rats of less genetic diversity have usually responded to skin graft-induced immunity in the same manner as those between more dissimilar rats. The less divergent group, however, revealed an occasional persistent functioning of parathyroid grafts even in the face of the sensitivity incidental to an accelerated reaction to skin. 5. The hypothesis is presented that the parathyroid tissue of the rats studied was deficient in effective transplantation antigens as compared with those of their skin. This weakness in apparent antigenicity may be a reflection of a deficiency in production, availability, or content of antigens in the tissue transferred.


A survey of Lactobacilli including pigmented strains from the oral cavity of the white rat.


Studies on Streptococcus pyogenes. IV. Effect of whole cells, M and C antigens, and other components of the cell on adrenalectomized rats. Heat-killed cells of Group A streptococci caused death of the adrenalectomized rat. While the adrenalectomized rat readily succumbed to intraperitoneal infection with living cells, death was due primarily to toxicity. The normal rat was highly resistant under either condition. For studies on the toxic materials, the cells of numerous serological types of group A streptococci, and of a Group B and a Group D streptococcus, were extracted with 0.1 N HCl at 100 degrees C. or by sonic oscillation. The extracts, containing macromolecular components, were subjected to chemical fractionation and purification. C substance and M protein of Group A streptococci released from the cell by sonic oscillation were toxic to the adrenalectomized rat in quantities of 1 mg./100 gm. rat. Death usually occurred within 2 hours. On the other hand, C substance and M protein released from the cell with HCl at 100 degrees C. were relatively non-toxic to the adrenalectomized rat. The sonic-extracted C substance of streptococcal Groups B, C, and D was also toxic. The toxic property of the C and M preparations was neutralized in vitro in each case by group and type-specific rabbit antiserum. Heterologous antiserum was without effect. Adrenalectomized rats which received homologous antiserum 18 hours before challenge were also resistant to the toxicity of the C and M preparations. Trypsin destroyed the toxic effect of the M protein preparations and was without effect on the toxicity of the C substance. The R antigen and a nucleoprotein component of Group A streptococci, preparations of protein from Groups B and D streptococci, and coagulase from Staphylococcus aureus were all found to be essentially non-toxicic for the adrenalectomized rat. Large quantities of peptone, crystalline albumin, and rabbit serum were also without effect.


Histochemical analysis of lesions in incipient dental caries.


Relationship between maternal ingestion of carbohydrate in the rat and dental caries susceptibility of the progeny.


Age variations in the number of mast cells in oral mucosa and skin of albino rats.


Relation of structure to the microhardness of human dentin.


Incidence and distribution of oral pleuropneumonialike and L organisms.


The caries-inhibiting effects of Na2HPO4 and CaHPO4.


The metabolism of silicon in the rat and its relation to the formation of artificial siliceous calculi. The urinary excretion of silicon in the rat was found to be enhanced beyond normal levels by the administration of various chemical forms of silicon. The excretion was enhanced to a much greater degree by the administration of ethyl silicate than by magnesium trisilicate, sodium metasilicate, or water glass. The tolerance level of rats to sustained daily doses of ethyl silicate fed via stomach tube was approximately 15 to 30 mg. of silicon per rat per day. Urinary silicon excretion was found to be a straight line function of the concentration of ethyl silicate administered, via stomach tube, with approximately 18 per cent of the administered silicon appearing in the urine at all levels tested. Using sustained dietary additions of ethyl silicate as a means of enhancing urine silicon levels, artificial siliceous urinary calculi were consistently produced on zinc pellets implanted in the bladders of rats.


Effect of human blood serum on tissue cultures. II. Development of resistance to toxic human serum in fibroblast-like cells (Earle's strain L) obtained from a C3H mouse. When NCTC clone 929 (strain L) cells were grown either continuously or at intervals in toxic human serum, they became resistant to the toxic substance or substances. The resistance developed to toxic human serum was passed from one cell generation to another; i.e., it was heritable. The resistance to toxic human serum, developed in strain L cells, was associated with a decrease in their ability to adsorb toxic substances from the serum. This suggests that there is a decrease in the number of loci in the resistant cells where toxic substances can be bound. The resistant cells differed from the sensitive cells in their morphological appearance, the increased frequency of minute chromosomes, and the increased adhesion to glass surfaces. The cells which developed a resistance to a particular toxic serum also resisted the toxic effects of some other sera, but not of all.


A stimulus-trace hypothesis for statistical learning theory.


A study of semantic generalization through the use of established conceptual mediations.


The relation between mean reward and mean reinforcement.


Comparison of predictions and estimates in a probability learning situation.


Renal glomerular lesions induced by rabbit antirat collagen serum in rats prepared with adjuvant. Renal glomerular lesions were induced by rabbit serum containing antibody to rat collagen injected intravenously into rats prepared with subcutaneously administered Freund adjuvant. Neither the anti-collagen serum nor the adjuvant alone induced the lesion. The lesions were characterized by diffuse glomerular injury with swelling, shredding, and fusion of the basement membranes, crescent formation, cellular proliferation, numerous multinuclear giant cells, and capillary hyaline thrombi. Various rabbit antisera, including those against fish collagen or rat serum failed to induce the renal lesion when substituted for anti-rat collagen serum. Also, anti-rat collagen serum absorbed with its homologous antigen, native rat collagen, failed to induce the lesion. Although complete adjuvant, i.e. with mycobacteria, in which normal serum was incorporated enhanced the glomerular lesion which resulted from intravenous injection of anti-collagen serum, the incomplete adjuvant without serum was sufficient. Comparison of the renal lesions induced by anti-collagen serum with nephrotoxic nephritis induced in rats by rabbit anti-kidney serum showed that they differ histologically. Also the antisera used to produce these two renal lesions differ immunologically. Antibodies to normal rabbit serum developed in rats injected intravenously with rabbit anti-rat collagen serum after preparation with adjuvant, but not when adjuvant was omitted. The pathogenesis of the renal injury is discussed as a manifestation of an antigen-antibody reaction, with nephritis occurring only after the adjuvant-stimulated antibody to the rabbit globulin has been formed in the rat and has reacted with the rabbit anti-rat collagen already fixed by its homologous antigen in the kidney.


Molecular growth requirements of single mammalian cells. III. Quantitative colonial growth of single S3 cells in a medium containing synthetic small molecular constituents and two purified protein fractions. Two purified serum protein fractions, fetuin and serum albumin, will replace whole or dialyzed serum in supporting the growth of single S3 HeLa cells in an otherwise chemically defined nutrient solution. In the serum-free medium, single S3 cells will form macroscopic colonies with essentially 100 per cent efficiency. The generation time of S3 cells in the serum-free medium is approximately 50 per cent greater than that observed in an optimal, serum-containing medium. All components of the serum-free medium are available commercially, except fetuin, which can easily be prepared in substantial quantities. The problem of the purity of the protein preparations and of their possible roles in promoting cell growth is discussed.


Some conditions governing the use of the cue-producing response as an explanatory device.


Growth of a motor skill as a function of distribution of practice.


Changes in performance as a function of shifts in the magnitude of reinforcement.