CHEMICAL AND IMMUNOLOGICAL COMPOSITION OF SURFACE STRUCTURES OF LISTERIA MONOCYTOGENES. Osebold, John W. (University of California, Davis), Ole Aalund, and Clarence E. Chrisp. Chemical and immunological composition of surface structures of Listeria monocytogenes. J. Bacteriol. 89:84-88. 1965.-A proteinlike surface substance was demonstrated on Listeria monocytogenes when an explanation was sought for the inagglutinability of some somatic antigens. The serological behavior of live bacteria and organisms subjected to heat, formalin, and trypsin was compared. The agglutination-inhibiting phenomenon was most pronounced with heat-killed (100 C) antigens. Trypsinization eliminated inagglutinability and increased sensitivity. Substances released by the enzyme had an ultraviolet-absorption peak at 260 mmu and showed a spot on paper chromatograms compatible with polypeptide. Inagglutinable cells combined with antibody because they could readily absorb antibodies from serum. After reaction with anti-Listeria serum, inagglutinable cells could be agglutinated by the addition of antiglobulin serum. It was hypothesized that heat inactivation of cells denatured the proteinaceous surface layer which interferred with the formation of a visible agglutination product but did not eliminate antigen-antibody reaction.


"GENETIC SPECIES" CONCEPT IN XANTHOMONAS. Friedman, S. (State University, Ghent, Belgium), and J. De Ley. "Genetic species" concept in Xanthomonas. J. Bacteriol. 89:95-100. 1965.-Deuterated, N(15)-labeled deoxyribonucleic acid (DNA) from Xanthomonas pelargonii forms distinct hybrids with ordinary DNA from X. hederae, X. juglandis, and X. carotae. Hybridization is less pronounced with X. phaseoli and X. begoniae. There is evidence that some hybridization occurs with X. vesicatoria, X. campestris, and X. tamarindi. These results favor the concept of a "genetic species," rather than a division of the genus into many separate species based almost entirely on phytopathogenic host specificity.


HOST-PARASITE RELATIONSHIPS WITH BRUCELLA NEOTOMAE. Gibby, Irvin W. (Cornell University Medical College, New York, N.Y.), and Anna M. Gibby. Host-parasite relationships with Brucella neotomae. J. Bacteriol. 89:9-16. 1965.-An investigation was undertaken to ascertain whether a vigorous parasitic state could be evolved by transfer procedures in the host-parasite system, Brucella neotomae versus the white mouse. Visible disease was not maintained and initial high doses were abruptly decreased if the parasite was serially transferred through host animals without the use of an adjuvant. Of several adjuvants tested, 5% mucin was the most effective in enhancing Brucella infections in the white mouse, and with this adjuvant a vigorous lethal disease was maintained in serial transfer. In one transfer series, bacterial colonies of a form different from B. neotomae were obtained. Substrains of the altered bacterial form were agglutinated by Brucella antiserum and exhibited other properties consistent with the genus, Brucella. Cultures and selected clones of this organism in low doses caused rapidly lethal disease in the white mouse in the absence of mucin. Also, the disease could be maintained in serial transfer as an acute lethal process without mucin. It is concluded that the host-parasite interaction was drastically altered with the emergence of a highly virulent parasite in an infectious state that had previously been relatively benign. Various related aspects of parasitism are discussed.


EFFECT OF PH ON HUMAN MYCOPLASMA STRAINS. Shepard, Maurice C. (U.S. Naval Medical Field Research Laboratory, Camp Lejeune, N.C.), and Carl D. Lunceford. Effect of pH on human Mycoplasma strains. J. Bacteriol. 89:265-270. 1965.-The optimal reaction of culture media for the cultivation of T-strain Mycoplasma of human origin was investigated. By use of a recently modified tryptic digest medium, the optimal reaction in either agar or fluid medium was found to be pH 6.0. In contrast, human classic (large-colony) Mycoplasma could be cultivated in agar or fluid medium over a rather broad pH range, and the influence of the reaction of the medium appeared to be primarily species-dependent. M. salivarium, for example, grew best in agar from pH 5.5 through 6.5. M. pneumoniae (Easton's agent) yielded largest colony numbers in agar and highest titers in broth at pH 8.0. In the case of T-strain Mycoplasma, both maximal colony numbers in agar and highest titers in fluid media were achieved at a reaction of pH 6.0. In addition, largest colony size of T-strain Mycoplasma was also achieved in agar at pH 6.0, and averaged 50 to 100% larger than that obtained by cultivation at pH 8.0 with the same medium. Although T-strains will develop in agar media over a pH range of from 5.0 through 10.0, the extremely small colony size and poor staining properties resulting from growth in an alkaline medium make their recognition in agar cultures difficult. Aerobic cultivation of T-strains was first achieved in agar adjusted to pH 5.5 to 6.0. In fluid medium, multiplication of T-strains occurred only within the limits of pH 5.0 through 8.0, with highest titers being reached at pH 6.0. Greater attention to the reaction of complete Mycoplasma media is stressed.


ELECTRON TRANSPORT IN BACILLUS POPILLIAE. Pepper, Rollin E. (Michigan State University, East Lansing), and Ralph N. Costilow. Electron transport in Bacillus popilliae. J. Bacteriol. 89:271-276. 1965.-Bacillus popilliae was found to be unique among aerobic microorganisms in that it was deficient in a hydrogen peroxide-scavenging system. Neither catalase nor peroxidase was found. At the same time, a system for producing hydrogen peroxide during oxidation of reduced nicotinamide adenine dinucleotide (NADH(2)) was consistently present in the soluble fraction of extracts of cells from older cultures. Cells harvested from 9-hr cultures did not produce a significant amount of peroxide. The soluble NADH(2) oxidase was apparently a flavoprotein, since it was stimulated by flavin nucleotides, insensitive to cyanide and azide, and inhibited by Atabrine. Also, difference spectra demonstrated the presence of a reducible flavin in the soluble fraction of cell extracts. The particulate fraction of cell extracts was shown by difference spectra to contain cytochrome b(1); the strong inhibition of NADH(2) oxidation by cyanide, azide, and carbon monoxide indicated that a terminal cytochrome oxidase was also present. This system was also flavin-dependent, since it was strongly inhibited by Atabrine. The specific activity of the NADH(2) oxidase in the particulate fraction was lower in extracts of cells from older cultures than in those from exponentially growing cultures. Cytochrome c was not found in extracts of these cells. It is believed that the increased participation of the hydrogen peroxide-generating NADH(2) oxidase in cells of older cultures may be responsible for the rapid loss in cell viability noted in stationary-phase cultures.


INDUCTION OF NONSPECIFIC RESISTANCE BY ENDOTOXIN IN UNRESPONSIVE MICE. Margherita, S. S. (Temple University School of Medicine, Philadelphia, Pa.), and H. Friedmann. Induction of nonspecific resistance by endotoxin in unresponsive mice. J. Bacteriol. 89:277-280. 1965.-Serratia marcescens endotoxin was tested for protective effect in animals of varying immunological competence. A quantity of endotoxin capable of inducing resistance in mice to a standardized challenge of Diplococcus pneumoniae was equally effective in animals immunologically paralyzed with a high dose of homologous pneumococcal polysaccharide. Failure of the unresponsive state to influence the degree of resistance induced by endotoxin suggests that a specific humoral factor does not play a significant role in the nonspecific resistance.


LAG PERIOD CHARACTERIZING THE ENTRY OF TRANSFORMING DEOXYRIBONUCLEIC ACID INTO BACILLUS SUBTILIS. Levine, James S. (Yale University, New Haven, Conn.), and Norman Strauss. Lag period characterizing the entry of transforming deoxyribonucleic acid into Bacillus subtilis. J. Bacteriol. 89:281-287. 1965.-The kinetics of appearance of transformants as a function of time of exposure to deoxyribonucleic acid (DNA) has been studied in Bacillus subtilis. A short lag period of approximately 1 min at 37 C is evident when the transformation is terminated with deoxyribonuclease. The length of this lag is independent of the genetic trait transferred. Moreover, the lag is unaffected by transforming DNA concentration, by the presence of homologous unmarked DNA, and by shearing and cross-linking of the transforming DNA. The lag shows a strong inverse temperature dependence. The energy of activation is 13.9 kcal. The lag is abolished when the transformation is terminated by washing instead of by addition of deoxyribonuclease. These results are taken to indicate an immediate adsorption of DNA to cells, followed by a deoxyribonuclease-sensitive period of 1 min, during which time the genetic trait is entering the cell. These results make feasible an investigation of the configuration assumed by the DNA molecule during entry into the cell.


CONFIGURATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID DURING ENTRY INTO BACILLUS SUBTILIS. Strauss, Norman (Yale University, New Haven, Conn.). Configuration of transforming deoxyribonucleic acid during entry into Bacillus subtilis. J. Bacteriol. 89:288-293. 1965.-A correlation was obtained between map distance and the length of the lag period preceding the appearance of pairs of genetic traits after the addition of deoxyribonucleic acid (DNA) to a competent culture of Bacillus subtilis. The results are taken to indicate that DNA enters competent cells in lengthwise fashion. The smallest length of transforming DNA which can participate in a recombination event, and the number of nucleotide pairs which enter the cell per unit time, have been estimated. The evidence indicates that only part of the lag period is devoted to the transport of DNA into the cell. The significance of these results with respect to the mechanism of entry of DNA into the cell is discussed.


TRANSDUCTION OF SPOROGENESIS IN BACILLUS SUBTILIS. Takahashi, I. (McMaster University, Hamilton, Ontario, Canada). Transduction of sporogenesis in Bacillus subtilis. J. Bacteriol. 89:294-298. 1965.-A number of asporogenous mutants of Bacillus subtilis were isolated and genetic analyses with these mutants were carried out by means of transduction mediated by bacteriophage PBS 1. The ability to form spores could be restored by transduction in all mutants examined, with the exception of Sp(-)H12-3, although they were not transformable under my experimental conditions. The results of reciprocal transduction suggest that genetic loci at which a mutation can affect sporulation are numerous. A spore marker (sp 1) was transduced jointly with prototrophy and some antibiotic resistance markers (streptomycin, erythromycin, and neomycin). Other spore markers (sp N2-2, sp 170-2, and sp H12-4) were also linked to auxotrophic markers (ser, tyr, and phe) at a fairly high frequency. This linkage relationship indicates that genes controlling the formation of spores are located along the chromosome far apart from each other. The result also suggests that blocks in a variety of biochemical processes may result in the expression of the asporogenous phenotype.


SYNTHESIS OF 2-DEMETHYL VITAMIN K2 AND THE CYTOCHROME SYSTEM IN HAEMOPHILUS. White, David C. (University of Kentucky College of Medicine, Lexington). Synthesis of 2-demethyl vitamin K(2) and the cytochrome system in Haemophilus. J. Bacteriol. 89:299-305. 1965.-The synthesis of the respiratory quinone, 2-demethyl vitamin K(2), is stimulated in Haemophilus parainfluenzae under conditions which provoke the synthesis of the cytochrome system. However, the various components of the electron-transport system can be formed in different proportions. The primary flavoprotein dehydrogenases are readily dissociated from the membrane without affecting the content of membrane-bound quinone, cytochrome b(1), or the cytochrome oxidases. These dehydrogenases must be membrane-bound to function, and each can be formed at a different rate. Molar ratios of various constituents of the electron-transport chain were calculated by use of reasonable extinction coefficients for the cytochromes. The molar ratio of quinone to cytochrome c(1) goes from 40 to 3 as the quinone content increases eightfold during the growth cycle. Similarly, the molar ratio of quinone to cytochrome oxidase a(2) varies from 27 to 17, and then increases to 31 as cytochrome oxidase a(1) assumes the oxidase function. The molar ratio of quinone to cytochrome b(1) remains 14 to 1 over a sixfold increase in both components measured in a mutant where cytochrome c(1) does not obscure cytochrome b(1). A similar consistency was noted between the quinone and cytochrome b(1) formation in the hemin-requiring H. influenzae.


EFFECT OF CHOLESTEROL ON THE SENSITIVITY OF MYCOPLASMA LAIDLAWII TO THE POLYENE ANTIBIOTIC FILIPIN. Weber, Morton M. (St. Louis University School of Medicine, St. Louis, Mo.), and Stephen C. Kinsky. Effect of cholesterol on the sensitivity of Mycoplasma laidlawii to the polyene antibiotic filipin. J. Bacteriol. 89:306-312. 1965.-The polyene antibiotic, filipin, inhibited growth and caused lysis of Mycoplasma laidlawii cells which had been cultured in the presence of cholesterol. The antibiotic did not inhibit growth and did not promote lysis of the organism when grown in the absence of cholesterol. These results constitute strong support for the contention that the presence of sterol in the cell membrane is a necessary prerequisite for polyene sensitivity. Higher concentrations of filipin were required to inhibit growth when serum was added to the assay medium than when it was absent. These results suggest binding of the antibiotic to some component in the serum and may partially account for the previous inability to demonstrate growth inhibition by low concentrations of the polyene antibiotics. The extent of growth inhibition due to filipin decreased upon prolonged incubation. Subculture in the presence of high concentrations of antibiotic indicated that the apparent reversal of inhibition was caused by emergence of a filipin-resistant cell population. It was also observed that cells, which originally were rapidly lysed by filipin and digitonin, were no longer responsive to the action of these agents upon incubation in sterol-free medium at 25 or 37 C for several hours. This effect could be prevented by keeping the cells at 2 C. These results may indicate that filipin-resistant cells carry out a metabolic conversion of membrane-localized sterol to a form which can no longer react with the antibiotic. Other possible causes of resistance, which cannot be excluded on the basis of the present data, are discussed.


ISOLATION OF MUTANTS AFFECTING TRYPTOPHANASE PRODUCTION IN ESCHERICHIA COLI. Gartner, Theodore K. (University of California, Davis), and Monica Riley. Isolation of mutants affecting tryptophanase production in Escherichia coli. J. Bacteriol. 89:313-318. 1965.-Mutants of Escherichia coli K-12 were isolated which appear to have suffered an alteration in the regulation system governing tryptophanase synthesis. A novel selection method was used to isolate tryptophanase mutants from tryptophan synthetase deletion mutants. Mutants were obtained which exhibited the following phenotypes either singly or in combination with others: constitutivity and inducibility at 13 C (wild type is not inducible at 13 C), constitutivity and hyperinducibility at 37 C. Mutation to constitutivity at 13 C seems to require two mutational changes of the wild type. Other mutants, presumably structural gene mutations, were isolated which lack tryptophanase activity under all conditions tested.


GENETIC ANALYSIS OF TRYPTOPHANASE MUTANTS OF ESCHERICHIA COLI. Gartner, Theodore K. (University of California, Davis), and Monica Riley. Genetic analysis of tryptophanase mutants of Escherichia coli. J. Bacteriol. 89:319-325. 1965.-The tryptophanase structural gene tna, and one of the tryptophanase regulation genes, R(1)tna, are closely linked, and lie between the pyr-E and ilv genes on the Escherichia coli chromosome. The mutant form of the regulation gene, which confers the properties of inducibility at 13 C, constitutivity at 37 C, and hyperinducibility at 37 C, is dominant over the wild form of the gene. Another regulation gene, R(2)tna, confers constitutivity at 13 C and does not map at the same locus as the R(2)tna gene.


FURTHER TAXONOMIC STUDIES ON STRAIGHT TO FLEXUOUS STREPTOMYCETES. Pridham, Thomas G. (Northern Regional Research Laboratory, Peoria, Ill.), and Allister J. Lyons. Further taxonomic studies on straight to flexuous streptomycetes. J. Bacteriol. 89:331-342. 1965.-The best way to handle streptomycete classification, nomenclature, and identification is through application of a genus-species-subspecies concept. To establish a species, principal criteria are morphology of chains of spores and nature of spore-wall surfaces. Subspecies can be differentiated one from another by other criteria, such as chromogenicity, colors of sporulating aerial mycelium and of vegetative mycelium, carbon-utilization patterns, and assessment of qualitative production of antibiotics and sensitivity and resistance to antibacterial antibiotics. A literature study and laboratory studies of some strains suggested that streptomycetes with straight chains of spores could easily be differentiated from those with flexuous chains of spores. An intensive study of about 75 holotype and potential neotype strains indicated that such a differentiation is difficult to accomplish with confidence. Only 19 of the strains had straight chains of spores. These strains are considered, at this time, to be members of the species Streptomyces venezuelae Ehrlich et al. Forty-two of the strains had flexuous chains of spores and were assigned to the species Streptomyces griseus (Krainsky) Waksman and Henrici. Six of the strains had unusual spore-chain morphology. Classic taxonomic procedures allowed the separation of all the strains into a number of categories. The results suggest that more precise information on relationships of strains and qualitative antibiotic production will allow clarification of their sub-specific status. Consideration of the results obtained with strains having aberrant morphology allows some speculation on ranges of variations in morphology that might be encountered with the streptomycetes.


MANNITOL AND MANNITOL DEHYDROGENASES IN CONIDIA OF ASPERGILLUS ORYZAE. Horikoshi, Koki (The Institute of Physical and Chemical Research, Tokyo, Japan), Shigeji Iida, and Yonosuke Ikeda. Mannitol and mannitol dehydrogenases in conidia of Aspergillus oryzae. J. Bacteriol. 89:326-330. 1965.-A sugar alcohol was isolated from the conidia of Aspergillus oryzae and identified as d-mannitol. Two types of d-mannitol dehydrogenases, nicotinamide adenine dinucleotide phosphate-linked and nicotinamide adenine dinucleotide-linked, were found in the conidia. Substrate specificities, pH optima, Michaelis-Menton constants, and the effects of inhibitors were studied. d-Mannitol was converted to fructose by the dehydrogenases. Synthesis of d-mannitol dehydrogenases was not observed during germination; the content of d-mannitol decreased at an early stage of germination. It was assumed, therefore, that d-mannitol might be used as the source of endogenous respiration and provide energy for the germination.


BIOCHEMICAL STUDIES OF THE SMOOTH-ROUGH MUTATION IN SALMONELLA MINNESOTA. Lüderitz, O. (Max-Planck-Institut für Immunbiologie, Frieburg, Germany), H. J. Risse, H. Schulte-Holthausen, J. L. Strominger, I. W. Sutherland, and O. Westphal. Biochemical studies of the smooth-rough mutation in Salmonella minnesota. J. Bacteriol. 89:343-354. 1965.-A comparative study of the O antigen from the smooth strain of Salmonella minnesota and of the two R antigens derived from two rough forms of S. minnesota (strains R 60 and R 345) has been carried out. The O-specific polysaccharide of the smooth form is composed of heptose, galactose, glucose, glucosamine, galactosamine, and ketodeoxyoctanoate (KDO). R 60 polysaccharide contains KDO, heptose, galactose, glucose, and glucosamine, whereas the R 345 polysaccharide contains only KDO, heptose, galactose, and glucose. Serologically, R 345 and R 60 polysaccharides belong to serogroups R I and R II, respectively. Enzymatic studies revealed that the acetylgalactosamine-synthesizing enzyme, uridine diphosphate-N-acetylglucosamine-4-epimerase, is present in wild-type and R 345 cells but is absent from R 60 cells. Two distinct polysaccharides were obtained from the R 345 cells: a polysaccharide derived from the R antigen (lipopolysaccharide) containing no galactosamine and exerting R specificity, and a soluble polysaccharide containing galactosamine and exerting O specificity. The structure of O and R antigens is discussed, together with the general significance of the results for the biosynthesis of the O antigens of the genus Salmonella.


FORMATION AND INTERRELATIONSHIPS OF TRYPTOPHANASE AND TRYPTOPHAN SYNTHETASES IN ESCHERICHIA COLI. Newton, W. Austin (University of California, Berkeley), and Esmond E. Snell. Formation and interrelationships of tryptophanase and tryptophan synthetases in Escherichia coli. J. Bacteriol. 89:355-364. 1965.-In addition to the classical tryptophan-repressible tryptophan synthetase (TSase-tr), tryptophan auxotrophs of Escherichia coli contain another distinct tryptophan synthetase (TSase-ti) which is induced by tryptophan and is identical with tryptophanase (TPase). Escherichia coli B (wild type) forms only TSase-tr when the growth medium lacks tryptophan. When tryptophan is supplied, parallel induction of TPase and TSase-ti occurs while TSase-tr is repressed. Antiserum prepared against purified TPase neutralized TPase and TSase-ti equally, but not TSase-tr. TPase-negative strains of E. coli do not form TSase-ti. Unlike TSase-tr, TSase-ti is not readily detected by whole-cell assays. In the tryptophan auxotroph, E. coli B/1t7, a direct correlation exists between the effectiveness of 4-, 5-, and 6-methyl-tryptophan in inducing TPase and in promoting growth in the presence of indole. In a mutant of this organism, E. coli B/1t7-A, which is constitutive for TPase, 5-methyl-tryptophan and other substrates of TPase increased the rate of growth on limiting indole, a result ascribed to their ability to inhibit degradation of tryptophan and to supply the 3-carbon side chain for synthesis of tryptophan by TPase. This organism produced maximal amounts of TPase when inocula from log-phase cells grown in tryptophan-supplemented minimal medium were allowed to undergo two cell generations in an enriched broth medium.


MYCOBACTERIUM LEPRAE IN MICE: MINIMAL INFECTIOUS DOSE, RELATIONSHIP BETWEEN STAINING QUALITY AND INFECTIVITY, AND EFFECT OF CORTISONE. Shepard, Charles C. (Communicable Disease Center, U.S. Public Health Service, Atlanta, Ga.), and Dorothy H. McRae. Mycobacterium leprae in mice: minimal infectious dose, relationship between staining quality and infectivity, and effect of cortisone. J. Bacteriol. 89:365-372. 1965.-The minimal infectious dose of Mycobacterium leprae in mouse foot pads was found to be on the order of 10 solidly staining bacilli. In a titration experiment, the actual number found was 3.4 to 34 solid bacilli, and the order of magnitude was confirmed by experience with inocula containing varying numbers of solidly staining leprosy bacilli from mouse passage and from clinical sources. The acid-fast staining quality of leprosy bacilli was related in a useful way to the subsequent rate at which bacillary growth appeared. When the proportion of solidly staining bacilli was high, the calculated generation time was shortest, and the lower the proportion, the longer the generation times. The results were in accord with the hypothesis that all viable bacilli are solid, and that when they die, most of them become nonsolid. Varying proportions of the dead bacilli, perhaps up to 10%, remain solid, at least temporarily. The growth curve of M. leprae in mice was followed in several experiments with total counts of acid-fast bacteria and determination of the ratio of solid bacilli. What had been called a maximal stationary phase was seen to consist of sequential phases of conversion of solid to nonsolid bacilli (death), reappearance of solid bacilli (growth), and conversion of solid to nonsolid bacilli (death). When cortisone was administered, leprosy bacilli grew somewhat more slowly during the logarithmic phase, but attained a higher level, especially of solidly staining bacilli.


NATURE OF BUTYRATE OXIDATION BY MYCOPLASMA HOMINIS. VanDemark, P. J. (Cornell University, Ithaca, N.Y.), and P. F. Smith. Nature of butyrate oxidation by Mycoplasma hominis. J. Bacteriol. 89:373-377. 1965.-Cell-free extracts of butyrate - grown Mycoplasma hominis strain O7, though lacking thiokinase activity on butyric acid, were found to activate butyrate via an acetyl-butyric thiophorase. These extracts also contained an aceto-coenzyme A (CoA) kinase, a butyryl-CoA dehydrogenase, a crotonase, a reduced nicotinamide adenine dinucleotide-specific beta-hydroxybutyryl-CoA dehydrogenase, and a thiolase. Thiolase activity was stimulated by the addition of magnesium ions. The presence of these enzyme activities in this Mycoplasma species supports the hypothesis that a fatty acid oxidation represents an energy source for the nonfermentative pleuropneumonia-like organisms.


ADSORPTION OF CATIONIC (BASIC) DYES BY FIXED YEAST CELLS. Giles, Charles H. (The University of Strathclyde, Glasgow, Scotland), and Robert B. McKay. Adsorption of cationic (basic) dyes by fixed yeast cells. J. Bacteriol. 89:390-397. 1965.-The adsorption of 10 typical cationic dyes on formalin-fixed yeast cells has been studied by determining isotherms, and the results are consistent with an ion-exchange mechanism. The adsorption on this complex substrate is similar to that on the simpler substrate, alumina. The dyes are probably aggregated when adsorbed, and the size of the aggregates increases with increase in the molecular weight of the dye ion. After considering the possible adsorption sites, and comparing the data with adsorption on simpler substrates, we suggest that the most important adsorption sites may be phosphate or other strongly acidic groups.


PURIFICATION OF STAPHYLOCOCCAL BETA-HEMOLYSIN AND ITS ACTION ON STAPHYLOCOCCAL AND STREPTOCOCCAL CELL WALLS. Chesbro, William R. (University of New Hampshire, Durham), Fred P. Heydrick, Roland Martineau, and Gail N. Perkins. Purification of staphylococcal beta-hemolysin and its action on staphylococcal and streptococcal cell walls. J. Bacteriol. 89:378-389. 1965.-After growth of bovine-derived strains of Staphylococcus aureus in a completely dialyzable medium, the beta-hemolysin in the culture supernatant fluids was purified by gradient-elution chromatography on cellulose phosphate. The purified hemolysin contained two components, demonstrable by immunodiffusion or electrophoresis, but was free from alpha-hemolysin, coagulase, Delta-hemolysin, enterotoxins A and B, glucuronidase, hyaluronidase, lipase, muramidase, Panton-Valentine leukocidin, phosphatase, and protease. The hemolysin was heat-labile and sulfhydryl-dependent, and the preparation was leukocidal for guinea pig macrophages. When rabbit red blood cell (RBC) stroma and staphylococcal or enterococcal cell walls were treated with the purified hemolysin, it liberated mucopolysaccharides from the rabbit RBC stroma, polysaccharides and mucopolysaccharides (or mucopeptides) from the staphyloccoal cell walls, and rhamnose, glucose, an unidentified monosaccharide, N-acetylglucosamine, and at least two polysaccharides from the enterococcal cell walls. The hemolytic and cell-wall degradative activities had similar thermal inactivation kinetics, pH optima, sedimentation coefficients, and chromatographic and electrophoretic mobilities; both required Mg and were inhibited by thiol-inactivating agents. Consequently, it seems likely that both activities are expressions of the same enzyme.


STUDIES OF STREPTOCOCCAL CELL WALLS. VII. CARBOHYDRATE COMPOSITION OF GROUP B CELL WALLS. Wittner, Masako K. (Presbyterian-St. Luke's Hospital, Chicago, Ill.), and James A. Hayashi. Studies of streptococcal cell walls. VII. Carbohydrate composition of group B cell walls. J. Bacteriol. 89:398-402. 1965.-Group B streptococcal cell walls contain 63% protein, 10% rhamnose, 18% hexose (mainly galactose, but also some glucose), 7% hexosamine (mainly glucosamine, but also galactosamine), and 3% muramic acid. The group and type antigens were extracted from isolated cell walls by acid treatment and enzymatic hydrolysis, and fractionated either with ethanol or on a diethylaminoethyl-cellulose column. Serological and chemical analyses of the fractions obtained in the two fractionation methods show that the group antigen is a rhamnose-rich polysaccharide and that the type antigen is rich in galactose and contains hexosamines.


EFFECT OF OXYGEN ON GROWTH AND THE SYNTHESIS OF BACTERIOCHLOROPHYLL IN RHODOSPIRILLUM MOLISCHIANUM. Sistrom, W. R. (University of Oregon, Eugene). Effect of oxygen on growth and the synthesis of bacteriochlorophyll in Rhodospirillum molischianum. J. Bacteriol. 89:403-408. 1965.-The basis of the strict anaerobiosis of the nonsulfur purple bacterium Rhodospirillum molischianum was investigated. The bacterium is unable to grow aerobically in the dark. In the light, the only immediate effect of oxygen is to inhibit the biosynthesis of bacteriochlorophyll and carotenoid pigments. Growth and protein synthesis are not inhibited directly, nor are the cells killed by oxygen. The inhibition of bacteriochlorophyll synthesis ultimately leads to inhibition of growth. Oxygen inhibits photopigment synthesis by means of a metabolic control system which is apparently identical with the one already known in facultatively aerobic species such as R. rubrum. Whole cells of R. molischianum respire in the dark; illumination inhibits respiration by about 50%. Cell-free extracts display significant levels of dark reduced nicotinamide adenine dinucleotide oxidase activity. Both these properties are in accord with the physiological behavior of the organism.


BIOCHEMICAL CHANGES DURING THE GROWTH OF FUNGI. II. ERGOSTEROL AND FATTY ACIDS IN PENICILLIUM ATROVENETUM. Van Etten, James L. (University of Illinois, Urbana), and David Gottlieb. Biochemical changes during the growth of fungi. II. Ergosterol and fatty acids in Penicillium atrovenetum. J. Bacteriol. 89:409-414. 1965.-Changes in the lipid constituents of Penicillium atrovenetum were studied during the growth and development of this fungus. The stages in development, as measured by the dry weight, were divided into four phases: lag, log, stationary, and death. The total fatty acids on a dry-weight basis increased from a minimum in the spores to a maximum near the end of the log phase of growth. The major fatty acids were palmitic, stearic, oleic, and linoleic. Myristic, pentadecanoic, palmitoleic, heptadecanoic, linolenic, arachidic, and heptadecenoic acids, together with two unidentified components, were also present in the fatty acid fraction. Compared to ungerminated spores, young mycelium contained a much lower percentage (on the basis of total fatty acids) of linoleic acid. There was a corresponding increase of oleic acid. Except for palmitic acid, which remained constant, the remaining fatty acids increased slightly. During subsequent growth of the fungus, linleic acid decreased, whereas the percentage of palmitic and stearic acids increased steadily from the lag phase to the end of the log phase. Ergosterol was the only sterol detected. The percentage of ergosterol, on a dry-weight basis, increased to a maximum at the start of the log phase and then steadily decreased. The following changes in lipids appear to be associated with the development and aging of fungi: (i) the presence of a relatively high content of nonsaponifiable lipid and ergosterol in the young mycelium, and their later decrease with age, and (ii) a shift from more unsaturated to less unsaturated fatty acids with age.


PROTOPLAST LYSIS AND INHIBITION OF GROWTH OF BACILLUS LICHENIFORMIS BY BACITRACIN. Snoke, John E. (University of California, Los Angeles), and Neal Cornell. Protoplast lysis and inhibition of growth of Bacillus licheniformis by bacitracin. J. Bacteriol. 89:415-240. 1965.-The growth of Bacillus licheniformis is inhibited by bacitracin, the antibiotic which the organism itself produces. The effect of the antibiotic is confined to the early growth phase of the organism. Bacitracin will produce a rapid lysis of protoplasts of B. licheniformis and Micrococcus lysodeikticus. The lytic process requires cadmium or zinc ions, and the rate and extent of lysis depends upon the amount of bacitracin and metal ion added. Although B. licheniformis and M. lysodeikticus differ markedly in their sensitivity to bacitracin, the protoplasts from these two organisms are equally susceptible to lysis by the antibiotic.


EFFECT OF AMINO ACIDS ON STEADY-STATE GROWTH OF A GROUP A HEMOLYTIC STREPTOCOCCUS. Davies, Helen C. (University of Pennsylvania, Philadelphia), Fred Karush, and Joanne H. Rudd. Effect of amino acids on steady-state growth of a group A hemolytic streptococcus. J. Bacteriol. 89:421-427. 1965.-A study has been made of amino acid utilization by a strain of type 4, group A streptococcus growing under steady-state conditions in a continuous-culture device and supplied with a completely synthetic medium. At a fixed growth rate, corresponding to a generation of time of 84 min, and with the pH maintained constant at 7.4, the bacterial turbidity was made dependent on the concentration of one of the amino acids of the defined medium. Under these conditions, the extracellular concentration of the limiting amino acid is fixed by the preset growth rate. The steady-state concentration of each of 14 essential l-amino acids was measured by means of C(14)-labeled amino acids in such limited cultures. At approximately equal turbidities, these concentrations ranged from 1.6 x 10(-6)m for methionine to 4.3 x 10(-4)m for glutamic acid. The rates of utilization of the amino acids ranged from 26 mmumoles per mg (dry weight) of bacteria per hr for histidine to 310 mmumoles per mg (dry weight) of bacteria per hr for glutamic acid. The percentage of the limiting amino acid used varied from 95% for threonine and methionine to 43% for gluamic acid. The rate of utilization of the limiting amino acid at unit concentration (tmoles per gram per hour per m) differed by a factor of 27 between extremes. These observations reflect the variation in the capacity of this streptococcal cell to take up and use different amino acids.


ACTION OF RIBONUCLEASE PREPARATIONS ON VIABLE YEAST CELLS AND SPHEROPLASTS. Schlenk, F. (Argonne National Laboratory, Argonne, Ill.), and J. L. Dainko. Action of ribonuclease preparations on viable yeast cells and spheroplasts. J. Bacteriol. 89:428-436. 1965.-Recrystallized pancreatic ribonuclease releases more than half of the ultraviolet-absorbing cellular constituents of Candida utilis and Saccharomyces cerevisiae. The presence of salts and buffer interferes with the action of the enzyme on the cytoplasmic membrane, and intracellular constituents show a similar inhibitory effect. Polymerized ribonuclease and conformational isomers cannot penetrate the pores of the yeast cell wall, but they are effective on the membrane of spheroplasts. All forms of the enzyme interfere strongly with the viability of the cells.


EFFECT OF BIOTIN ON FATTY ACID DISTRIBUTION IN ESCHERICHIA COLI. Gavin, John J. (Rutgers, The State University, New Brunswick, N.J.), and Wayne W. Umbreit. Effect of biotin on fatty acid distribution in Escherichia coli. J. Bacteriol. 89:437-443. 1965.-Biotin deficiency causes changes in the composition and distribution of the fatty acids in cell wall-cell membrane fractions of Escherichia coli T 94A. Most notable among the fatty acid changes are decreased amounts of unsaturated fatty acids, the presence of unsaponifiable lipid material, and the lack of a lipopolysaccharide fraction in the cell wall-cell membrane. Also, though the hexane-extractable material from the lipoprotein fraction of biotin-adequate cells will transfer glucose from water to hexane, the same material from biotin-deficient cells will not.


COMPARISON OF THE CELL-WALL COMPOSITION OF MORPHOLOGICALLY DISTINCT ACTINOMYCETES. Yamaguchi, Tatsuro (The University of Tokyo, Tokyo, Japan). Comparison of the cell-wall composition of morphologically distinct actinomycetes. J. Bacteriol. 89:444-453. 1965.-Cell-wall composition of various morphologically distinct actinomycetes was studied to determine the relationship, if any, between cell-wall composition and morphological criteria in actinomycete taxonomy. The methods used were similar to those of Cummins and Harris. At least five types of cell-wall composition were obtained; however, these were not always correlated with groupings by the conventional classification system. For instance, the sporangium-forming actinomycetes, Actinoplanaceae, had three types of cell-wall composition; the composition of cell walls of Promicromonospora, Micromonospora, and Microbispora was the same as, or similar to, that of Actinomyces, Actinoplanes, and Streptosporangium, respectively; Chainia, Actinopycnidium, Actinosporangium, and Microellobosporia had the same cell-wall composition as Streptomyces, whereas that of Streptoverticillium was slightly different. Possible implications of cell-wall composition and morphological differentiation of hyphae for the taxonomy and phylogeny of actinomycetes are also discussed.


ADANSONIAN ANALYSIS AND DEOXYRIBONUCLEIC ACID BASE COMPOSITION OF SERRATIA MARCESCENS. Colwell, R. R. (Georgetown University, Washington, D.C.), and M. Mandel. Adansonian analysis and deoxyribonucleic acid base composition of Serratia marcescens. J. Bacteriol. 89:454-461. 1965.-A total of 33 strains of Serratia marcescens were subjected to Adansonian analysis for which more than 200 coded features for each of the organisms were included. In addition, the base composition [expressed as moles per cent guanine + cytosine (G + C)] of the deoxyribonucleic acid (DNA) prepared from each of the strains was determined. Except for four strains which were intermediate between Serratia and the Hafnia and Aerobacter group C of Edwards and Ewing, the S. marcescens species group proved to be extremely homogeneous, and the different strains showed high affinities for each other (mean similarity, S = 77%). The G + C ratio of the DNA from the Serratia strains ranged from 56.2 to 58.4% G + C. Many species names have been listed for the genus, but only a single clustering of the strains was obtained at the species level, for which the species name S. marcescens was retained. S. kiliensis, S. indica, S. plymuthica, and S. marinorubra could not be distinguished from S. marcescens; it was concluded, therefore, that there is only a single species in the genus. The variety designation kiliensis does not appear to be valid, since no subspecies clustering of strains with negative Voges-Proskauer reactions could be detected. The characteristics of the species are listed, and a description of S. marcescens is presented.


PHYSICAL PROPERTIES OF CHICK INTERFERON. Kreuz, Leo E. (The Johns Hopkins University School of Medicine, Baltimore, Md.), and Allan H. Levy. Physical properties of chick interferon. J. Bacteriol. 89:462-469. 1965.-The sedimentation coefficient, diffusion coefficient, and molecular weight of chick-embryo interferon were determined by zone centrifugation and equilibrium sedimentation in cesium chloride density gradients, and by chromatography on Sephadex G-100 columns. Purified interferon is not available in quantities sufficient to permit direct analysis by chemical or physical means; its relative concentrations were determined, therefore, by bioassay. I(125)-human serum albumin was used as an internal reference in all experiments. The sedimentation coefficient of chick-embryo interferon is 2.2 to 2.3S; the diffusion coefficient is 9.5 x 10(-7) cm(2)sec(-1). A molecular weight of 26,000 was calculated from the sedimentation and diffusion coefficients, and a range of 25,000 to 34,000 daltons was obtained from equilibrium-sedimentation analyses.


PERSISTENT POLIOVIRAL INFECTION OF INTACT HUMAN AMNIOTIC MEMBRANE WITHOUT APPARENT CYTOPATHIC EFFECT. Gresser, Ion (Children's Hospital Medical Center, Boston, Mass.), Charles Chany, and John F. Enders. Persistent polioviral infection of intact human amniotic membrane without apparent cytopathic effect. J. Bacteriol. 89:470-475. 1965.-Poliovirus type I multiplied in organ cultures of the intact amniotic membrane. The behavior of the virus in this system differed markedly from that observed in monolayer cultures of trypsinized amnion cells, since replication of new virus was demonstrable throughout a period as long as 3 months, extensive cellular destruction was not apparent, and only a small proportion of cells were found to be infected at a given time. It seems likely that the chronicity of infection is conditioned essentially by the persistent insusceptibility to polioviral infection of the large majority of cells (relative to monolayer cultures) rather than to interferon, which was present in the culture fluids. It was postulated that the greater suceptibility of cultured amnion cells to infection, as compared with cells of the intact membrane, is due in part to the enhancement of phagocytic activity attendant upon trypsinization and cultivation of the amnion cells in monolayers. Amniotic and chorionic membranes thoroughly washed free from blood contain and release in vitro antibody to poliovirus I and other viruses. Appreciation of this phenomen may be important in attempts to isolate viruses from infected tissues.


ENZYMATIC ACTION ON THE CAPSULAR MATERIAL PRODUCED BY PSEUDOMONAS AERUGINOSA OF CYSTIC FIBROSIS ORIGIN. Doggett, Robert G. (Texas Institute for Rehabilitation and Research, Houston), Gunyon M. Harrison, Richard N. Stillwell, and Everett S. Wallis. Enzymatic action on the capsular material produced by Pseudomonas aeruginosa of cystic fibrosis origin. J. Bacteriol. 89:476-480. 1965.-An enzymatic study of the action of alpha- and beta-amylases on the slime envelope produced by Pseudomonas aeruginosa originating from patients with cystic fibrosis (CF) has been reported. It has been shown that these two enzymes in combination prevent the formation of the abnormal ethanol-benzene insoluble mucoid present in the slime layer of the above organism of CF origin. Evidence is also given which suggests that this organism can serve as a tool for studies of chemical abnormalities in the mucous metabolism of CF individuals.


FINE STRUCTURE OF RICKETTSIA QUINTANA CULTIVATED IN VITRO AND IN THE LOUSE. Ito, Susumu (Harvard Medical School, Boston, Mass.), and J. W. Vinson. Fine structure of Rickettsia quintana cultivated in vitro and in the louse. J. Bacteriol. 89:481-495. 1965.-Usually rod-shaped, Rickettsia quintana cells measure about 0.2 to 0.5 mu wide and up to 1.6 mu long. The rickettsiae have both an outer cell wall, about 80 A thick, and a plasma membrane, about 70 A thick, each of which is trilaminar. Occasional vesicular invaginations of the plasma membrane occur. The nuclear material, distributed in irregular zones throughout the cytoplasm, appears as a loose network of fine fibrils when postfixed with uranyl acetate and as thick strands or clumps after routine OsO(4) fixation. The cytoplasm is densely packed with numerous granules, presumably ribosomes, about 150 A in diameter. Histochemical studies revealed the presence of both ribonucleic and deoxyribonucleic acids. During binary fission, a constricting furrow is formed by the cell wall and plasmalemma. No difference in fine structure was observed between R. quintana propagated on cell-free media and in the louse.


OBSERVATIONS ON THE GROUP C STREPTOCOCCAL BACTERIOPHAGE AND LYTIC ENZYME SYSTEM. Fox, Eugene N. (La Rabida-University of Chicago Institute, Chicago, Ill.), and Masako K. Wittner. Observations on the group C streptococcal bacteriophage and lytic enzyme system. J. Bacteriol. 89:496-502. 1965.-The phage-associated lytic enzyme of group C streptococci was assayed by measuring the solubilized portion of radioactive cell walls. By this sensitive assay system, the induced synthesis of the lytic enzyme was observed intracellularly during phage infection; at least half of the total enzyme was synthesized and remained intracellular during the eclipse period, and was then released with the liberation of mature phage. Lytic enzyme could be detected in only two of eight lysogenic strains during temperate-phage production after ultraviolet induction. Virulent phage purified by density-gradient centrifugation contained lytic enzyme presumably associated with the virus per se. No hyalyronidase was detected in association with the phage, nor was this enzyme induced during phage synthesis. Variant strains of group C streptococci, no longer serologically active, were isolated as phage-resistant mutants. These strains still adsorbed the phage, but without subsequent virus reproduction, indicating that the group polysaccharide was not the primary receptor for the virus.


BIOLOGY OF BUDDING BACTERIA. 3. FINE STRUCTURE OF RHODOMICROBIUM AND HYPHOMICROBIUM SPP. Conti, S. F. (Dartmouth Medical School, Hanover, N.H.), and Peter Hirsch. Biology of budding bacteria. III. Fine structure of Rhodomicrobium and Hyphomicrobium spp. J. Bacteriol. 89:503-512. 1965.-The ultrastructure of 14 strains of hyphomicrobia, and of Rhodomicrobium vannielii, was investigated by means of electron microscopy of thin sections. The majority of the strains of hyphomicrobia possessed a well-developed internal membrane system, which appeared to be derived by invagination from the cytoplasmic membrane. The subcellular organization of the hyphomicrobia and R. vannielii was investigated.


SYNCHRONY OF DIVISION IN CLONAL MICROCOLONIES OF ESCHERICHIA COLI. Hoffman, Heiner (New York University, New York, N.Y.), and Michael E. Frank. Synchrony of division in clonal microcolonies of Escherichia coli. J. Bacteriol. 89:513-517. 1965.-A common pattern of synchrony of division was found in 18 clonal microcolonies studied by means of time-lapse photomicrography. One of these is described in detail. Cell counts at 1-min intervals were carried into the 11th generation of a microcolony photographed for 5.25 hr. The counts indicated that an early synchrony of division occurred which apparently was gradually dissipated during the course of cultivation. However, when times of cell division for each generation were plotted, it became apparent that each generation conformed to a distribution curve which gradually changed in its characteristics from one generation to the next. The range of time during which the divisions occurred increased from 16 min in the 6th generation to at least 74 min in the 11th. Overlap between distribution curves increased from 3 min between the 7th and 8th generations, where it initially occurred, to at least 20 min between the 10th and 11th. Skewness of the distribution curve progressed from a negative value (-0.943) in the 5th generation to a positive value (+0.339) in the 10th. Genealogical identification of the first and last quarters of the cells to divide within a generation revealed, early in the course of cultivation, a cell subline which was dividing in a pattern of synchrony independent of the colony as a whole. The characteristics of this synchronous pattern, however, gradually progressed with each new generation toward those of the microcolony as a whole.


INTRACELLULAR GROWTH OF HISTOPLASMA CAPSULATUM. Howard, Dexter H. (University of California, Los Angeles). Intracellular growth of Histoplasma capsulatum. J. Bacteriol. 89:518-523. 1965.-Under a variety of experimental conditions, the generation time of Histoplasma capsulatum in mammalian histiocytes was remarkably constant. The rate of intracellular growth of five different isolates of H. capsulatum was very similar. The generation time of the fungus within guinea pig cells was nearly the same as it was within mouse cells under comparable conditions. Preliminary exposure of yeast cells to specific antibodies and complement did not affect intracellular proliferation of the fungus during the first 24 hr after phagocytosis by mouse histiocytes. The rate of growth of the fungus within cells from immunized mice and guinea pigs was comparable to that in cells from normal animals. This latter result was not altered by preliminary exposure of the fungus to hyperimmune serum and complement.


ROSETTE-LIKE ARRANGEMENT OF POLYOMA VIRUS PARTICLES IN INFECTED MOUSE-EMBRYO CELLS.


BETA-HEMOLYTIC STREPTOCOCCI WITH GROUP A AND TYPE II CARBOHYDRATE ANTIGENS. Jablon, James M. (National Children's Cardiac Hospital, Miami, Fla.), Betty Brust, and Milton S. Saslaw. beta-Hemolytic streptococci with group A and type II carbohydrate antigens. J. Bacteriol. 89:529-534. 1965.-Ten strains of beta-hemolytic streptococci with unusual somatic antigens were isolated from excised tonsils or throat cultures or both. Acid extracts of these strains reacted with commercial group A and group F antisera, but gave no reaction when tested with 35 type-specific group A antisera. Serum cross-absorption and agar-gel diffusion studies established the identity of the reactive antigens as the specific carbohydrates of group A and of type II. The latter antigen has been found in many strains of group F streptococci. The organisms do not possess the group-specific carbohydrate of group F, and the reaction with the commercial group F antiserum is due to the presence of type II antibody in the antiserum. The organisms give a stronger reaction to fluorescein-conjugated type II antiserum than to group A. However, the organisms have only one group-specific carbohydrate, that of group A, and, tentatively, should be classified as such.


STREPTOMYCIN INDUCTION OF PREMATURE LYSIS OF BACTERIOPHAGE-INFECTED MYCOBACTERIA.


OCCURRENCE OF PYRUVIC ACID IN THE CAPSULAR POLYSACCHARIDE OF KLEBSIELLA RHINOSCLEROMATIS.


BIOSYNTHESIS OF NICOTINIC ACID BY MYCOBACTERIUM TUBERCULOSIS.


STABILITY OF SPECIFIC BACTERIOPHAGE STORED ON FILTER-PAPER STRIPS FOR THE RAPID IDENTIFICATION OF PASTEURELLA PESTIS.


EFFECT OF HEMATIN ON THE RECOVERY OF BACILLUS ANTHRACIS AND RELATED ORGANISMS.


LEPTOSPIRAL LYSIS BY LIPIDS OF RENAL TISSUE AND MILK.


TAXONOMIC VALUE OF INFRARED SPECTRA.

TAXONOMIC VALUE OF INFRARED SPECTRA.


NEOMYCIN ACTIVITY IN SERUM AND PLASMA IN THE CYLINDER PLATE ASSAY.


MEDICAL GENETICS 1963.

MEDICAL GENETICS 1963.


METABOLIC EFFECTS OF HUMAN GROWTH HORMONE DERIVED FROM EMBALMED BODIES.


THE AQUATIC EVOLUTION OF MAN.

THE AQUATIC EVOLUTION OF MAN.


PROPOSED INVOLUNTARY COMMITMENT FOR ALCOHOLICS.


MEDICAL ECONOMICS AND ALTERNATIVES TO HOSPITAL CARE.


THE UROLOGICAL ASPECTS OF PYELONEPHRITIS.


TANGIBLE BENEFITS.

TANGIBLE BENEFITS.


THE ROLE OF COMMITMENT IN THE TREATMENT OF THE ALCOHOLIC.


HISTORY AND DEVELOPMENT OF THE ASSOCIATION OF RETIRED DOCTORS OF MEDICINE OF BROWARD COUNTY, FLORIDA, INC.


MANAGEMENT OF NASAL FRACTURE.

MANAGEMENT OF NASAL FRACTURE.


WORKMEN'S COMPENSATION PROGRAM.

WORKMEN'S COMPENSATION PROGRAM.


THE DEVELOPMENTAL ASPECTS OF THE SKELETAL SYSTEM WITH REFERENCE TO SKELETAL ABNORMALITIES.


RECOGNITION OF SURGICAL EMERGENCIES IN THE NEWBORN.


PSYCHIATRIC ASPECTS OF THERAPEUTIC ABORTION.


PECTUS EXCAVATUM AND CARDIOVASCULAR ABNORMALITIES.


SUPERNORMAL EXCITATION OF THE HUMAN HEART.


CARDIAC CATHETERIZATION AND INDICATOR-DILUTION CURVES.


DIRECT NEEDLE PUNCTURE IN THE DIAGNOSIS OF RENAL MASS LESIONS IN INFANTS.


FURTHER STUDIES ON THE STRUCTURE AND PROPERTIES OF HUMAN HAEMOGLOBINS MODIFIED BY DIGESTION WITH THE CARBOXYPEPTIDASES.


POLYRIBOSOMES AND THE LOSS OF HEMOGLOBIN SYNTHESIS IN THE MATURING RETICULOCYTE.


MULTIPLE MOLECULAR FORMS OF BETA-GALACTOSIDASE.


BINDING OF BASIC PROTEINS TO DNA.

BINDING OF BASIC PROTEINS TO DNA.


THE CHANGE OF VOLUME ASSOCIATED WITH THE HELIX-COIL TRANSITION IN POLY-GAMMA-BENZYL-L-GLUTAMATE.


MECHANISM OF DEGRADATION OF DNA BY ENDONUCLEASE I FROM ESCHERICHIA COLI.


RECOMBINATION OF HUMAN ADULT AND CANINE HAEMOGLOBINS AT NEUTRAL PH.


THE THERMAL DENATURATION OF NUCLEOPROTEIN IN BOAR SPERM.


BINDING OF TRANSFER RIBONUCLEIC ACID TO RIBOSOMES ENGAGED IN PROTEIN SYNTHESIS: NUMBER AND PROPERTIES OF RIBOSOMAL BINDING SITES.


ON THE TRANSCRIPTION OF THE TRYTOPHAN OPERON IN ESCHERICHIA COLI. I. THE TRYPTOPHAN OPERATOR.


ON THE KINETICS OF THE HELIX-COIL TRANSITION OF POLYPEPTIDES IN SOLUTION.


AN ELECTRON MICROSCOPIC STUDY OF A DNA-RIBOSOME COMPLEX FORMED IN VITRO.


RIBONUCLEASE DIGESTION OF R17 VIRAL RNA.


THE DETACHMENT AND MATURATION OF CONSERVED LAMBDA PROPHAGE DNA.


RNA SYNTHESIS IN CELLS INFECTED WITH THE MENINGOPNEUMONITIS AGENT.


PRESIDENTIAL ADDRESS.

PRESIDENTIAL ADDRESS.


OUR NEW PRESIDENT.

OUR NEW PRESIDENT.


MILITARY PROGRESS THROUGH SCIENTIFIC ACHIEVEMENT--ARMY.


MILITARY PROGRESS THROUGH SCIENTIFIC ACHIEVEMENT--NAVY.


BENEFICIAL FALLOUT FROM AEROSPACE MEDICAL RESEARCH.


PUBLIC HEALTH SERVICE: INCREASED RESPONSIBILITIES, RAPID CHANGE.


THE MEDICAL SERVICE PROGRAM OF THE VETERANS ADMINISTRATION.


PRESIDENTIAL RESPONSIBILITY, SUCCESSION, AND TRAGEDY: WOODROW WILSON 1856-1924.


THE IMAGE OF MILITARY MEDICINE.

THE IMAGE OF MILITARY MEDICINE.


MILITARY MEDICINE IN THE MEDICAL CURRICULUM.


CONTRIBUTIONS OF THE ARMED FORCES EPIDEMIOLOGICAL BOARD TO MILITARY PROGRESS.


AFFECTIONS OF THE AORTIC ARCH.

AFFECTIONS OF THE AORTIC ARCH.


ENZYMATIC TREATMENT OF HYPERCHOLESTEROLEMIA AND ATHEROSCLEROSIS.


APPARENT REFRACTORINESS TO CHLOROQUINE, PYRIMETHAMINE, AND QUININE IN STRAINS OF PLASMODIUM FALCIPARUM FROM VIETNAM.


COINS IN THE ESOPHAGUS: A DEPARTURE FROM CONVENTIONAL THERAPY.


SOME PERTINENT COURT DECISIONS IN THE FIELD OF LEGAL MEDICINE.


A MATTER OF PRIORITY.

A MATTER OF PRIORITY.


INTERNATIONAL SYMPOSIUM ON END RESULTS OF CANCER THERAPY. SURVIVAL TABLES.


THE HYPERKINETIC CIRCULATORY STATES. 2. CONCLUSION.


VENOUS THROMBOSIS AND PULMONARY EMBOLISM.


THE FUTURE OF ANATOMICAL EPONYMS.

THE FUTURE OF ANATOMICAL EPONYMS.


CORRECTABLE RENAL HYPERTENSION. XII. PROGNOSIS.


CARCINOMA OF THE AMPULLA OF VATER. CASE REPORT OF TREATMENT WITH PANCREATICODUODENECTOMY.


A CLINICOPATHOLOGICAL CONFERENCE FROM THE OHIO STATE UNIVERSITY HOSPITAL, COLUMBUS,OHIO.


MATERNAL DEATHS INVOLVING LOWER NEPHRON NEPHROSIS.


VOCATIONAL REHABILITATION AND THE DOCTOR.


A SECOND VIRUS-CARRYING TISSUE CULTURE STRAIN (EB2) OF LYMPHOBLASTS FROM BURKITT'S LYMPHOMA.


INDIVIDUAL TASK PERFORMANCE IN A CHANGING SOCIAL STRUCTURE.


SOCIOMETRIC PATTERNS IN HYSTERICAL CONTAGION.


TWO DIMENSIONS OF COHESIVENESS IN SMALL GROUPS.


PRIOR REASSURANCE OF GROUP CONSENSUS AS A FACTOR IN PRODUCING RESISTANCE TO PERSUASION.


TYPE OF GROUP AND THE PROBABILITY OF INITIATING ACTS.


SELF IDENTITY AND REFERENT OTHERS.

SELF IDENTITY AND REFERENT OTHERS.


THE EFFECTS OF LEADER SELECTION ON GROUP PERFORMANCE.


INTERACTION STRUCTURE, FRUSTRATION, AND THE EXTENSIVENESS AND INTENSITY OF AGGRESSION.


ANGIOTENSIN AMIDE; A BRIEF REVIEW.

ANGIOTENSIN AMIDE; A BRIEF REVIEW.


HYPERTENSION; EVALUATION AND TREATMENT.

HYPERTENSION; EVALUATION AND TREATMENT.


THREE-PHASE TREATMENT PROGRAM FOR ALCOHOL ADDICTION.


OSTEOPOROSIS; PRESENT CONCEPTS.

OSTEOPOROSIS; PRESENT CONCEPTS.


NUTRITION IN CARDIOVASCULAR DISEASE.

NUTRITION IN CARDIOVASCULAR DISEASE.


EFFECT OF COMMON ODORS ON PATIENTS WITH RESPIRATORY ALLERGIES.


CLINICOPATHOLOGIC CONFERENCE.

CLINICOPATHOLOGIC CONFERENCE.


USE OF LOW MOLECULAR DEXTRAN IN ACUTE MYOCARDIAL INFARCTION.


THE ETIQUETTE OF REFERRALS.

THE ETIQUETTE OF REFERRALS.


SUBCLAVIAN-INTERNAL CAROTID BYPASS FOR THROMBOSIS OF LEFT COMMON CAROTID ARTERY.


ACINIC CELL CARCINOMA OF THE PAROTID GLAND.


CLINICOPATHOLOGIC CONFERENCE SPONSORED BY THE SECTION ON PATHOLOGY, STATE MEDICAL SOCIETY OF WISCONSIN.


TREATMENT OF CARDIAC ARRHYTHMIAS.

TREATMENT OF CARDIAC ARRHYTHMIAS.


VOLKMANN'S CONTRACTURE OF THE LOWER EXTREMITIES. A REPORT OF 14 CASES ARISING IN CHILDREN.


THE PAINFUL HIP.

THE PAINFUL HIP.


A NEW HEMORRHOIDECTOMY CLAMP; (A NEW TECHNIQUE).


A NEW CONCEPTION ON THE CAUSATION, PREVENTION AND ARREST OF VARICOSE VEINS, VARICOCELE AND HAEMORRHOIDS.


HEMORRHOIDS-GAS-CONSTIPATION AND THE RECTALATOR.


VOLVULUS ALONG THE GASTRO-INTESTINAL TRACT.


A STUDY OF BENIGN EPITHELIAL LESIONS OF THE LARGE INTESTINE. I. NOMENCLATURE OF COMMON BENIGN EPITHELIAL LESIONS (POLYPS) OF THE LARGE INTESTINE.


EFFECT OF INTRANASAL DEXAMETHASONE PHOSPHATE ON THE ADRENAL FUNCTION OF PATIENTS WITH PERENNIAL ALLERGIC RHINITIS.


GRISEOFULVIN: BIOLOGY AND CLINICAL USEFULNESS. A REVIEW.


LAWRENCE JAMES HALPIN, 1909-1965.

LAWRENCE JAMES HALPIN, 1909-1965.


A FACTOR ANALYTICALLY DERIVED SCALE FOR RATING PSYCHIATRIC PATIENTS IN OCCUPATIONAL THERAPY. I. DEVELOPMENT.


PATIENT EVALUATION OF OCCUPATIONAL THERAPY PROGRAMS.


NOTES ON THE RELATIONSHIP BETWEEN THE THERAPEUTIC AND ANTICOMPLEMENTARY EFFECTS OF HEPARIN IN ACQUIRED HEMOLYTIC ANEMIA.


THE OCCUPATIONAL THERAPIST OF THE FUTURE. PLANNING FOR.


OBSERVATIONS ON THE INCIDENCE OF ALLERGIC DISORDERS IN A VILLAGE IN THE UNITED ARAB REPUBLIC.


IMPROVING TACTILE DISCRIMINATION OF THE BLIND. A NEUROPHYSIOLOGICAL APPROACH.


LOOM ADAPTATION.

LOOM ADAPTATION.


ART AS THERAPY IN THE CURATIVE WORKSHOP.


THE PRESIDENT'S ADDRESS.

THE PRESIDENT'S ADDRESS.


ABSORPTION SPECTRA OF INCLUSION BODIES IN BETA-THALASSEMIA.


CENTRAL NERVOUS SYSTEM INVOLVEMENT BY LEUKEMIA IN CHILDREN. II. THERAPY WITH INTRATHECAL METHOTREXATE.


CENTRAL NERVOUS SYSTEM INVOLVEMENT BY LEUKEMIA IN CHILDREN. I. RELATIONSHIP TO SYSTEMIC LEUKEMIA AND DESCRIPTION OF CLINICAL AND LABORATORY MANIFESTATIONS.


EXPECTANCY FOR LIFE IN CHRONIC LYMPHATIC LEUKEMIA.


CHRONIC ERYTHROCYTIC HYPOPLASIA FOLLOWING PERNICIOUS ANEMIA.


THE EFFECT OF AMELIORATION OF ANEMIA ON THE SYNTHESIS OF FETAL HEMOGLOBIN IN SICKLE CELL ANEMIA.


CONTINUOUS FREE-FLOW FRACTIONATION OF CELLULAR CONSTITUENTS IN RAT BONE MARROW.


ELECTRON MICROSCOPY OF THE RED CELLS IN ERYTHROPOIETIC PORPHYRIA.


THE HISTOCHEMICAL STUDY OF ZINC CONTENT IN GRANULOCYTES IN NORMAL ADULTS AND IN HEMATOLOGIC DISORDERS.


THE COURSE OF EXPERIMENTALLY INDUCED HEMOLYTIC ANEMIA IN A PRIMAQUINE-SENSITIVE CAUCASIAN. A CASE STUDY.


EXCESS HEMOLYSIS IN SUBJECTS WITH SEVERE IRON DEFICIENCY ANEMIA ASSOCIATED AND NONASSOCIATED WITH HOOKWORM INFECTION.


DEMONSTRATION OF ISOANTIGENIC RECEPTORS ON HUMAN EPIDERMAL CELLS AND ESTABLISHED CELL LINES.


SURGERY OF THE DEEP FASCIA OF THE LEG. I. SUBLUXATION OF THE DEEP FASCIA OF THE LEG, ITS RELATION TO PRIMARY VARICOSE VEINS, AND ITS SURGICAL TREATMENT.


SURGERY OF THE DEEP FASCIA OF THE LEG. II. DECOMPRESSION FASCIOTOMY FOR THE TREATMENT OF SWOLLEN INDURATED LEGS DUE TO DEEP VENOUS THROMBOSIS OF THE CALF.


LATE BOWEL OBSTRUCTION DUE TO KINKING OF THE DAMAGED LOOP FOLLOWING REDUCTION OF A STRANGULATED HERNIA.


SPONTANEOUS RUPTURE OF HEPATOCELLULAR CARCINOMA.


BENIGN DUODENOCOLIC FISTULA.

BENIGN DUODENOCOLIC FISTULA.


EXTRAHEPATIC PORTAL-VENOUS OBSTRUCTION.

EXTRAHEPATIC PORTAL-VENOUS OBSTRUCTION.


GUT-STONES IN A MECKEL'S DIVERTICULUM.

GUT-STONES IN A MECKEL'S DIVERTICULUM.


SILENT PROSTATIC CARCINOMA PRESENTING AS BILATERAL URETERIC OBSTRUCTION.


GREAT TEACHERS OF SURGERY IN THE PAST. SIR ERNEST FINCH (1884-1960).


SOLITARY SIGMOID DIVERTICULITIS.

SOLITARY SIGMOID DIVERTICULITIS.


SURGERY AND INTESTINAL MOTILITY.

SURGERY AND INTESTINAL MOTILITY.


EMPYEMA THORACIS.

EMPYEMA THORACIS.


CONN'S SYNDROME AND SUBARACHNOID HAEMORRHAGE.


THYMECTOMY FOR AUTOIMMUNE DISEASE.

THYMECTOMY FOR AUTOIMMUNE DISEASE.


LIVER INJURIES IN NORTHERN IRELAND.

LIVER INJURIES IN NORTHERN IRELAND.


B.C. HOSPITALS LEARN TO LIVE WITH LABOR.


WHAT'S CURRENT IN PLANNING, DESIGN, RESEARCH, ENGINEERING.


QUOTA PROGRAM FREES ACTIVE BEDS.

QUOTA PROGRAM FREES ACTIVE BEDS.


HOMOTRANSPLANTATION OF TISSUE. AN OVER-VIEW.


CHOANAL ATRESIA. I. MEDICAL ASPECTS OF A SERIOUS ANOMALY.


PNEUMOCOCCIC SEGMENTAL (LOBAR) PNEUMONIA. ITS TREATMENT WITH A SINGLE INJECTION OF PROCAINE PENICILLIN G.


CHOANAL ATRESIA. II. SURGICAL MANAGEMENT.


THE INNOCENT (FUNCTIONAL) CARDIAC MURMURS OF CHILDHOOD.


INFANTILE SCURVY.

INFANTILE SCURVY.


DIASTROPHIC DWARFISM. A LITTLE KNOWN DISEASE ENTITY.


CARBOHYDRATE METABOLISM IN PEOPLE LIVING IN CHRONIC HYPOXIA.


ATTITUDES OF DIABETIC BOYS AND GIRLS TOWARDS DIABETES.


VASCULAR DISEASE IN DIABETES.

VASCULAR DISEASE IN DIABETES.


LONG-TERM OBSERVATIONS ON ORAL HYPOGLYCEMIC AGENTS IN DIABETES; THE EFFECT OF CARBUTAMIDE AND TOLBUTAMIDE.


A COMPARISON OF INSULIN-LIKE ACTIVITY CONTAINED IN THE SERUM PROTEINS OF NORMAL SUBJECTS AND DIABETIC PATIENTS.


PATTERNS OF DECREASE OF FREE FATTY ACIDS DURING GLUCOSE TOLERANCE TESTS.


CARBOHYDRATE METABOLISM IN MYOCARDIAL INFARCTION: BEHAVIOR OF BLOOD GLUCOSE AND FREE FATTY ACIDS AFTER GLUCOSE LOADING.


INACTIVATION OF INSULIN BY ADIPOSE TISSUE.


COMPARISON OF PANCREATIC AND STEROID DIABETES IN RESPECT TO TUMOR GROWTH AND GLYCOSURIA.


A NEW CARBOHYDRATE SOLUTION FOR TESTING GLUCOSE TOLERANCE.


AMITRIPTYLINE IN CHILDHOOD DEPRESSIONS.

AMITRIPTYLINE IN CHILDHOOD DEPRESSIONS.


WILL UNIVERSAL DISABILITY COMPENSATION DECREASE HOSPITAL POPULATIONS?


MEPHENOXALONE AS A PSYCHOTHERAPEUTIC AGENT IN CHILDREN WITH CEREBRAL DYSRHYTHMIAS: A PRELIMINARY REPORT.


A DIFFERENT APPROACH IN A PSYCHIATRIC CLINICAL CLERKSHIP.


CLINICAL NEUROPATHOLOGICAL CONFERENCE.

CLINICAL NEUROPATHOLOGICAL CONFERENCE.


THE DEFENSE OF INSANITY--A PLEA FOR ITS ABANDONMENT.


THE CHRONIC MENTAL PATIENT IN THE COMMUNITY.


COMPARISON OF THREE PHENOTHIAZINES ON CHRONIC PSYCHOTIC BEHAVIOR.


BEHAVIORAL AND METABOLIC CHANGES ASSOCIATED WITH ADMINISTRATION OF TETRAETHYLTHIURAM DISULFIDE (ANTABUSE).


EFFECT OF CLOMIPHENE ON BLASTOCYSTS DURING DELAYED IMPLANTATION IN THE RAT.


EFFECT OF ANTIESTROGENS ON ESTROGEN-INDUCED OVA IMPLANTATION IN THE OVARIECTOMIZED RAT.


GENERATION AND PROPAGATION OF UTERINE ACTIVITY IN SITU.


GONORRHEAL SALPINGITIS: VIEWS ON TREATMENT AND PROGNOSIS.


STUDIES ON UTERINE FLUID. I. DISC ELECTROPHORETIC AND DISC-GEL OUCHTERLONY ANALYSES OF RAT UTERINE FLUID.


THE IMPREGNATORS. (WILLIAM PANCOAST), (ADDISON DAVIS HARD).


ELECTRON MICROSCOPY OF THE DEVELOPING DECIDUA.


BIOTELEMETRY AS AN ADJUNCT TO THE STUDY OF OVARIAN PHYSIOLOGY.


THE SPLIT EJACULATE: ITS USE IN THE MANAGEMENT OF MALE INFERTILITY.


AUTO-OCCLUSION OF THE OVIDUCTS: A POTENTIAL MEANS OF STERILIZATION.


THE EFFECTS OF NEW SYNTHETIC PROGESTOGENS ON ENDOMETRIOSIS IN THE HUMAN FEMALE.


THE ESOPHAGUS WITH COLUMNAR EPITHELIUM, ITS CLINICAL SIGNIFICANCE.


OLDER PERSONS' JUDGEMENT OF THE PASSAGE OF TIME OVER THE LIFE-SPAN.


THE ROLE OF SPATIAL CECTORCARDIOGRAPHY IN THE DIAGNOSIS OF HEART DISEASE.


ANTITESTIS ANTIBODY: ITS INHIBITION OF PREGNANCY.


RESPONSE OF ISOLATED HUMAN FALLOPIAN TUBE TO OXYTOCIN: A PRELIMINARY REPORT.


TREATMENT OF COMMON DUCT STONES IN THE ELDERLY POOR-RISK PATIENT.


KIDNEY FUNCTION STUDIES IN OLD MEN AND WOMEN.


MESTRANOL IN OVULATION SUPPRESSION.

MESTRANOL IN OVULATION SUPPRESSION.


DIAGNOSIS AND TREATMENT OF HEART FAILURE IN THE ELDERLY.


THE ROLE OF ANTICOAGULANT THERAPY IN THE TREATMENT OF MYOCARDIAL INFARCTION.


GERONTOLOGY PROGRAM OF PHS.

GERONTOLOGY PROGRAM OF PHS.


REGRESSION RATE OF VASCULAR EFFECTS INDUCED BY PREGNANCY AND BY NORETHYNODREL-MESTRANOL.


NORETHINDRONE-MESTRANOL AS A THERAPEUTIC AGENT.


OTHER ASPECTS OF DEPRESSION.

OTHER ASPECTS OF DEPRESSION.


ASSOCIATION OF BRONCHOGENIC CARCINOMA AND PULMONARY TUBERCULOSIS.


IS IT EPILEPSY OR SYNCOPE?

IS IT EPILEPSY OR SYNCOPE?


OPHTHALMIC SCREENING TEST FOR INFANTS AND VERY YOUNG CHILDREN.


MANAGEMENT OF THE COLLAGEN DISEASES.

MANAGEMENT OF THE COLLAGEN DISEASES.


MANAGEMENT OF PRIMARY ARTERIAL HYPERTENSION. 2.


MEDICAL AND SOCIAL MANAGEMENT OF THE MENTALLY RETARDED.


THE VALSALVA MANEUVER: A VERSATILE CLINICAL TOOL.


CONGENITAL ABSENCE OF PECTORALIS MUSCLES.


BONE MARROW ASPIRATION AND BIOPSY.

BONE MARROW ASPIRATION AND BIOPSY.


PREPARING A CHILD FOR HOSPITALIZATION.

PREPARING A CHILD FOR HOSPITALIZATION.


CONTACT LENSES AND THE UNCONSCIOUS PATIENT.


MANAGEMENT OF PEPTIC ULCER.

MANAGEMENT OF PEPTIC ULCER.


REPAIR OF INJURIES TO THE FACIAL SOFT TISSUES.


OSTEITIS DEFORMANS.

OSTEITIS DEFORMANS.


OFFICE TREATMENT OF VARICOSE VEINS AND ULCERS.


THE MULTIPLE-UNIT HOSPITAL-CLINIC PHARMACY. 2. PHARMACY LOGISTICS; ROBINSON-PATMAN ACT; REPACKING; PREPACKAGING.


THE ROLE OF THE OR SUPERVISOR AND THE NURSE CONSULTANT.


THE ROLE OF INFECTION-CONTROL CHEMICALS IN HOSPITAL SANITATION. I. THE NEED FOR ADMINISTRATIVE CONTROL.


USE OF HOSPITAL PNEUMATIC TUBE SYSTEM FOR TRANSPORT OF BLOOD CHEMISTRY SPECIMENS.


LABORATORY PERSONNEL SHOULD BE CONSIDERED A PART OF THE C. S. TEAM.


SAFETY STARTS WITH PURCHASING.

SAFETY STARTS WITH PURCHASING.


THE PROFESSIONAL DEVELOPMENT OF THE PURCHASING AGENT.


AN EVALUATION OF A DISPOSABLE WATERPROOF DRAW SHEET.


A GUIDE TO SELECTING STAND-BY POWER EQUIPMENT.


ARE DEPARTMENTAL COSTS COMPARABLE BETWEEN REGIONS?II.


THE LIABILITY OF THE SURGICAL NURSE. II. REPORTS OF SOME POOR NURSING SERVICES THAT RESULTED IN LAWSUITS.


GOOD HOSPITAL PUBLICITY: AN IMPORTANT FACTOR OF THE PUBLIC RELATIONS PROGRAM.


THE NATURE OF AUTHORITY IN HOSPITAL ADMINISTRATION.


WORK IMPROVEMENT IMPROVES WITH WORK.

WORK IMPROVEMENT IMPROVES WITH WORK.


PRODUCTIVE TIME OF EMPLOYEES CAN BE IMPROVED.


HOSPITAL LAUNDRIES--IN OR OUT?

HOSPITAL LAUNDRIES--IN OR OUT?


EXPERIENCES OF A PRIVATE CATHOLIC HOSPITAL RESEARCH LABORATORY.


DEATH BY VOMITUS ASPIRATION; VA HOSPITAL NOT LIABLE.


THE NUTRITIONIST AND THE TEAM APPROACH.

THE NUTRITIONIST AND THE TEAM APPROACH.


A DISASTER PLAN TESTED.

A DISASTER PLAN TESTED.


EFFECTIVE SUPERVISION REQUIRES LEADERSHIP.


THE MIGRANT WORKER.

THE MIGRANT WORKER.


MANNING THE EMERGENCY DEPARTMENT. II.

MANNING THE EMERGENCY DEPARTMENT. II.


THE TEAM ASSIGNMENT PLAN.

THE TEAM ASSIGNMENT PLAN.


PROGRAMMED INSTRUCTION AND AUTOMATED EDUCATION FOR HOSPITAL AND OTHER MEDICAL PERSONNEL.


THE SUPPLY OF NURSES: AN ECONOMIST'S VIEW.


STRIP PACKAGING AND SIMPLIFIED RECORDS BRING NEW EFFICIENCY IN NARCOTICS DISTRIBUTION.


PLANNING HOSPITAL INTERIOR DESIGN.

PLANNING HOSPITAL INTERIOR DESIGN.


SOLVING SEVEN CLEANING PROBLEMS.

SOLVING SEVEN CLEANING PROBLEMS.


HOW CIVIL RIGHTS REGULATIONS AFFECT HOSPITALS.


HOW OUTSIDE CONTROLS SHAPE HOSPITAL DECISIONS.


HOW HOSPITALS AND HOMES FOR THE AGED CAN HELP EACH OTHER.


THE ASSISTANT ADMINISTRATOR: HIS JOB AND HIS FUTURE.


AN OUTSIDE AUDIT MADE A GOOD CHARGE SYSTEM BETTER.


RESTORATIVE SERVICES FOR PATIENTS OUTSIDE THE GENERAL HOSPITAL.


NURSING HOME FOOD SERVICE: WORKSHOP FOCUSES ON CONSULTATION TECHNIQUES FOR IMPROVING FOOD QUALITY AND ECONOMY.


RELATIONSHIP OF VOLUNTARY HEALTH AGENCIES TO COLLEGE HEALTH PROGRAMS.


UTILIZING COLLEGE HEALTH EDUCATION RESOURCES: RESEARCH REVIEW AND COMMENTARY.


PULMONARY TUBERCULOSIS IN HONG KONG UNIVERSITY STUDENTS.


DISHONESTY IN COLLEGE STUDENTS.

DISHONESTY IN COLLEGE STUDENTS.


RAPID MASS ELECTROCARDIOGRAPHIC SCREENING IN COLLEGE STUDENTS.


INFORMAL HEALTH EDUCATION FOR FRESHMEN.

INFORMAL HEALTH EDUCATION FOR FRESHMEN.


MEDICAL AND MINOR SURGICAL PROBLEMS IN ATHLETICS.


A STUDY OF THE OUTCOME OF PSYCHOTHERAPY IN A UNIVERSITY MENTAL HEALTH SERVICE.


UNDERGRADUATE SMOKING HABITS AND RELATED BEHAVIOR. II. ATTITUDES TOWARD THE SMOKING-CANCER RELATIONSHIP AND PERSONALITY TRAITS.


PSYCHOTHERAPEUTIC ASPECTS OF UNSUCCESSFUL SUICIDE ATTEMPTS.


HODGKIN'S DISEASE IN ROOMMATES.

HODGKIN'S DISEASE IN ROOMMATES.


CLINICAL AND PHYSIOLOGIC ASPECTS OF OBESITY.


DIET AND CARDIOVASCULAR DISEASE.

DIET AND CARDIOVASCULAR DISEASE.


CAN CHANGES IN THE AMERICAN DIET PREVENT CORONARY HEART DISEASE?


A RATIONAL APPROACH TO DIET AND DIABETES.


PROGRAMMED INSTRUCTION MATERIALS ON DIABETES.


WHAT IS PROGRAMMED INSTRUCTION?

WHAT IS PROGRAMMED INSTRUCTION?


PHENYLALANINE CONTENT OF FRUIT.

PHENYLALANINE CONTENT OF FRUIT.


MEDICAL EVALUATION OF THE PSYCHIATRIC PATIENT.


THE ROLE OF THE FAMILY DOCTOR IN CHILDHOOD AND ADOLESCENCE.


UNCERTAINTIES IN GERIATRIC DATA. II. ORGAN SIZE.


RHEOGRAPHIC REGIONAL METHOD FOR EVALUATION OF CEREBRAL AND OCULAR CIRCULATION IN CARDIAC AND CEREBROVASCULAR DISEASE.


AORTOGRAPHY PROBLEMS IN THE AGED PATIENT.


THE CHALLENGING PROCESSES OF AGING: SOME CAUSES AND REMEDIES.


GERIATRIC PATIENTS DO HAVE ORAL SURGICAL PROBLEMS.


BEHAVIORAL IMPROVEMENT IN THE OLDER SCHIZOPHRENIC PATIENT: DRUG AND SOCIAL THERAPIES.


CANCER IN THE ELDERLY.

CANCER IN THE ELDERLY.


A BISACODYL SUPPOSITORY AS AN ENEMA SUBSTITUTE.


LOW BACK PAIN AND SCIATICA IN ELDERLY PATIENTS.


IMMUNIZATION--1964.


THE USE OF STEROIDS IN THE TREATMENT OF HAY FEVER AND BRONCHIAL ASTHMA.


GROWTH AND DEVELOPMENT.

GROWTH AND DEVELOPMENT.


CHEMOTHERAPY OF VIRAL INFECTIONS.

CHEMOTHERAPY OF VIRAL INFECTIONS.


NURSING HOMES IN ARKANSAS.

NURSING HOMES IN ARKANSAS.


RENAL DISEASE AND FAILURE.

RENAL DISEASE AND FAILURE.


FUNCTIONAL STIMULUS LEARNING AS RELATED TO DEGREE OF PRACTICE AND MEANINGFULNESS.


UCS INTENSITY AND THE ASSOCIATIVE STRENGTH OF THE EYELID CR WITH A MASKED CONDITIONING PROCEDURE.


PREDICTION OF FREE RECALL FROM WORD-ASSOCIATION MEASURES: A REPLICATION.


SEQUENTIAL PATTERNS AND MAXIMIZING.

SEQUENTIAL PATTERNS AND MAXIMIZING.


ACQUISITION AND EXTINCTION AFTER INITIAL TRIALS WITHOUT REWARD.


THE ROLE OF THE CORRECTIVE THERAPIST IN THE TOTAL PSYCHIATRIC REHABILITATION APPROACH.


FURTHER RAMIFICATIONS OF THE NON-MEDICAL REHABILITATION CONSULTANT'S ROLE IN P.M.R.S.


INFLUENCE OF RESPONSE DISCRIMINABILITY ON STIMULUS DISCRIMINABILITY.


STRENGTH TESTING POSITIONS MOST EFFECTIVE FOR DETERMINING PRE-EXISTING KNEE INJURY.


ANAGRAM SOLUTION TIMES: A FUNCTION OF THE "RULEOUT" FACTOR.


CHANGING CONCEPTS OF PATIENT CARE.

CHANGING CONCEPTS OF PATIENT CARE.


EFFECTS OF REPEATED BRIEF EXPOSURES ON THE GROWTH OF A PERCEPT.


ASSOCIATION AND DISCRIMINATION IN PAIRED-ASSOCIATES LEARNING.


AN INTRODUCTION TO MOVEMENTS FOR THE NEUROLOGICALLY IMPAIRED.


EXTINCTION AS A FUNCTION OF THE ORDER OF PARTIAL AND CONSISTENT REINFORCEMENT.


DEVELOPMENTAL AND ADAPTED PHYSICAL EDUCATION IN SCHOOLS AND COLLEGES.


NUMBER AND TYPE OF AVAILABLE INSTANCES IN CONCEPT LEARNING.


EFFECTS OF DELAY INTERVALS AND MEANINGFULNESS ON VERBAL MEDIATING RESPONSES.


DIFFERENTIAL CONDITIONING, EXTINCTION, AND SECONDARY REINFORCEMENT.


A COMPARISON OF TWO PAYOFF FUNCTIONS ON MULTIPLE-CHOICE DECISION BEHAVIOR.


INCREASING CREATIVITY BY FREE-ASSOCIATION TRAINING.


REVERSAL AND NONREVERSAL SHIFT LEARNING IN NORMAL CHILDREN AND RETARDATES OF COMPARABLE MENTAL AGE.


PREDICTION ON THE BASIS OF CONDITIONAL PROBABILITIES.


RELATION BETWEEN STIMULUS INTENSITY AND OPERANT RESPONSE RATE AS A FUNCTION OF DISCRIMINATION TRAINING AND DRIVE.


CAN A HEAD INJURY CAUSE A MENINGIOMA?

CAN A HEAD INJURY CAUSE A MENINGIOMA?


FATIGUE.

FATIGUE.


GALL-STONE ILEUS.

GALL-STONE ILEUS.


CLINICAL TRIAL OF A NEW DIURETIC: ETHACRYNIC ACID.


CAECO-COLIC INTUSSUSCEPTION FOLLOWING APPENDICECTOMY.


THE HYPOTENSIVE ACTION OF BETHANIDINE SULPHATE.


OTOGENIC BRAIN ABSCESS: A STUDY OF EIGHTY CASES.


THE PROBLEM OF NOISE IN THE ROYAL NAVY AND ROYAL MARINES.


HEALING OF EXPERIMENTAL PERFORATIONS OF THE TYMPANIC MEMBRANE.


EXOGENOUS COLLAGEN IN THE CLOSURE OF TYMPANIC MEMBRANE PERFORATIONS: A PRELIMINARY REPORT.


MYRINGOPLASTY--A CRITICAL STUDY.

MYRINGOPLASTY--A CRITICAL STUDY.


A CASE OF NEURILEMMOMA OF THE TONGUE.

A CASE OF NEURILEMMOMA OF THE TONGUE.


PHENYLEPHRINE: A VASOCONSTRICTOR FOR USE DURING HALOTHANE ANAESTHESIA.


MALIGNANT TUMOURS OF THE EAR.

MALIGNANT TUMOURS OF THE EAR.


CHROMOBLASTOMYCOSIS (VERRUCOUS DERMATITIS).


OVARIAN CANCER.

OVARIAN CANCER.


ECTOPIC PREGNANCY. A REVIEW OF 507 CASES.


DOUBLE BLIND STUDY WITH DIAZEPAM, CHLORDIAZEPOXIDE AND PLACEBO IN THE TREATMENT OF PSYCHONEUROTIC ANXIETY.


MENOPAUSAL ESTROGEN REPLACEMENT?

MENOPAUSAL ESTROGEN REPLACEMENT?


DC DEFIBRILLATION--ITS CLINICAL USE.

DC DEFIBRILLATION--ITS CLINICAL USE.


GEORGIA'S DEVELOPING COMMUNITY HOSPITAL PSYCHIATRIC PROGRAM.


FACIAL PAIN.

FACIAL PAIN.


THE PRIMARY MALABSORPTION SYNDROME.

THE PRIMARY MALABSORPTION SYNDROME.


RECURRENT PTERYGIUM: USE OF TOPICAL TRIETHYLENE THIOPHOSPHORAMIDE.


TYPHOID FEVER IN AN IMMUNIZED CHILD: A CASE REPORT.


RADIOLOGIC SEMINAR XXXIV. TRICHOBEZOAR.

RADIOLOGIC SEMINAR XXXIV. TRICHOBEZOAR.


ABUSES AND USES OF ULTRASOUND.

ABUSES AND USES OF ULTRASOUND.


CLINICOPATHOLOGICAL CONFERENCE LXI: CONDUCTED BY THE DEPARTMENT OF PATHOLOGY, MISSISSIPPI BAPTIST HOSPITAL, JACKSON, MISSISSIPPI.


SIGNIFICANCE OF RECENT ADVANCES IN LIPID AND CARBOHYDRATE METABOLISM.


SOME NEW ASPECTS OF AORTIC STENOSIS.

SOME NEW ASPECTS OF AORTIC STENOSIS.


VERTIGO.

VERTIGO.


PHARMACIST AND PHYSICIAN RELATIONSHIPS.

PHARMACIST AND PHYSICIAN RELATIONSHIPS.


THE OFFICE MANAGEMENT OF PATHOLOGICAL AGGRESSIVENESS IN ADOLESCENTS.


IDIOPATHIC THROMBOCYTOPENIC PURPURA.

IDIOPATHIC THROMBOCYTOPENIC PURPURA.


TERATOGENIC DRUGS. TREATMENT FOR MOTHER; THREAT TO CHILD.


SOUTH CAROLINA'S MATERNAL MORTALITY FOR 1962.


EYE OPENER FOR THE GENERAL PRACTITIONER.


POISONOUS PLANTS IN SOUTH CAROLINA. 1.

POISONOUS PLANTS IN SOUTH CAROLINA. 1.


ACCIDENTAL POISONING OF THE MONTH. ARSENICAL INSECTICIDE.


THE EFFECT OF PROLONGED ATELECTASIS OR ISCHEMIA UPON THE LUNGS OF GROWING DOGS.


PSEUDOSARCOMA OF THE ESOPHAGUS.

PSEUDOSARCOMA OF THE ESOPHAGUS.


SUTURELESS BRONCHIAL STUMP CLOSURE; AN EXPERIMENTAL STUDY.


TUBERCULOUS BRONCHOBILIARY FISTULA. REPORT OF AN UNUSUAL CASE WITH DEMONSTRATION OF THE FISTULOUS TRACT BY PERCUTANEOUS TRANSHEPATIC CHOLANGIOGRAPHY.


INTRATHORACIC MENINGOCELE.

INTRATHORACIC MENINGOCELE.


AN EXAMINATION OF THE MERITS AND THE INTRINSIC LIMITATIONS OF EXFOLIATIVE CYTOLOGY IN 465 CASES OF LUNG CANCER.


COSTAL CHONDRITIS WITH ASSOCIATED OSTEOMYELITIS.


STUDIES OF SPINAL ARTERIAL BED PRESSURE FOLLOWING EXTENSIVE MOBILIZATION OF THE AORTA FROM THE POSTERIOR PARIETES.


BILATERAL OSTEOCHONDROMA OF THE RIBS IN AN INFANT: AN UNUSUAL CAUSE OF CYANOSIS.


POLYOSTOTIC FIBROUS DYSPLASIA (ALBRIGHT'S SYNDROME); A CASE REPORT.


HEMOLYSIS OF BLOOD IN THE PERICARDIUM: THE MAJOR SOURCE OF PLASMA HEMOGLOBIN DURING TOTAL BODY PERFUSION.


OBSTRUCTION OF A MOUSSEAU-BARBIN TUBE FROM PROLAPSED ESOPHAGEAL MUCOSA.


OBLITERATION OF THE PRINCIPAL ORIFICE OF THE STENOTIC MITRAL VALVE: A RARE FORM OF "RESTENOSIS".


AN OPERATIVE TECHNIQUE WITH CARDIOPULMONARY BYPASS FOR PRODUCING CHRONIC ANOXEMIA IN CALVES.


EXPERIMENTAL TECHNIQUES FOR THE PREVENTION OF SLUDGING OF ERYTHROCYTES IN EXTRACORPOREAL CIRCULATION.


TWO SPECIALLY DESIGNED CLAMPS FOR USE IN THE SURGICAL RELIEF OF VASCULAR EMBOLISM AND THROMBOSIS.


HEART VALVE SURGERY.

HEART VALVE SURGERY.


MANNITOL AND EXTRACORPOREAL CIRCULATION.


EXPERIMENTAL INDUCTION OF CHRONIC COMPLETE HEART BLOCK.


THE PEDICLE OPERATION FOR CORONARY INSUFFICIENCY: TECHNIQUE AND PRELIMINARY RESULTS.


THE SYNDROME OF PULMONARY ARTERY ANEURYSMS, PULMONARY ARTERY THROMBI, AND PERIPHERAL VENOUS THROMBI.


DIASTOLIC PRESSURE-VOLUME RELATIONSHIPS OF THE LEFT VENTRICLE DURING HYPOTHERMIA.


WEBER LATERALIZATION AT UNINVOLVED FREQUENCIES IN PATHOLOGICAL EARS.


THE OTOLARYNGOLOGIC ASPECTS OF MYASTHENIA GRAVIS.


THE CRICOPHARYNGEUS MUSCLE, AN ELECTROMYOGRAPHIC STUDY IN THE DOG.


PAROTITIS AND FACIAL PARALYSIS IN MID-LINE GRANULOMA.


TREATMENT OF CHRONIC RECURRENT PAROTITIS.


ULTRASTRUCTURE OF A HUMAN NASAL PAPILLOMA.


CHRONIC BACTERIAL SINUSITIS. (CONTRIBUTION TO ITS TREATMENT WITH STREPTOKINASE-STREPTODORNASE).


IRON DEFICIENCY IN OTOLARYNGOLOGY.

IRON DEFICIENCY IN OTOLARYNGOLOGY.


STENOSIS OF THE EXTERNAL AUDITORY MEATUS SECONDARY TO CHRONIC EXTERNAL OTITIS TREATED BY A SIMPLIFIED SURGICAL METHOD: REPORT OF CASE.


A NEW ESOPHAGEAL FEEDING TUBE.

A NEW ESOPHAGEAL FEEDING TUBE.


DRUM AND MIDDLE EAR GRAFTS IN CATS. II.

DRUM AND MIDDLE EAR GRAFTS IN CATS. II.


THE EFFECT OF SURGICAL TRAUMA IN OTOSCLEROSIS. A REPORT OF FOUR CASES.


SOME EMOTIONAL ASPECTS OF DEAFNESS.

SOME EMOTIONAL ASPECTS OF DEAFNESS.


A CASE STUDY OF COLLAPSE OF THE EAR CANAL DURING AUDIOMETRY.


THE DISTRIBUTION OF NUCLEIC ACIDS IN COCHLEAR CELLS.


BACTERIOLOGICAL INVESTIGATION IN BRONCHOSCOPY.


CARCINOMA OF THE MAXILLARY SINUS WITH RESIDUAL THOROTRAST.


OBSERVATIONS IN TREATING TWENTY-FIVE CASES OF CHOANAL ATRESIA BY THE TRANSPALATINE APPROACH.


INFANTILE SPASMS ASSOCIATED WITH HYPSARHYTHMIA.


PHENYLKETONURIA IN MANITOBA 1964.

PHENYLKETONURIA IN MANITOBA 1964.


EXAMINATION OF PRE-SCHOOL CHILDREN OF WELFARE FAMILIES IN WINNIPEG.


PARATAXIS IN MEDICINE.

PARATAXIS IN MEDICINE.


SOME GENERAL OBSERVATIONS ON HOSPITAL AND MEDICAL SERVICES IN MANITOBA.


THE ROYAL COMMISSION ON HEALTH SERVICES--A CHALLENGE TO THE DOCTOR.


THE IMPORTANCE OF AN ADEQUATE PRE-SCHOOL EXAMINATION.


HOSPITALS REPORT ON MEDICAL CARE IN HOSPITALS.


INGESTION OF LAUNDRY BLEACH.

INGESTION OF LAUNDRY BLEACH.


"COUGH SYNDROME" WITH SUGGESTIONS AS TO THE POSSIBLE ROLE PLAYED BY CEREBRAL ATHEROSCLEROSIS.


TREAT OR WATCH?AN EVALUATION OF ELECTIVE TREATMENT OF CLINICALLY UNINVOLVED NODES IN MALIGNANT MELANOMA.


PHENYLKETONURIA IN VICTORIA.

PHENYLKETONURIA IN VICTORIA.


A SIMPLIFIED METHOD FOR DETERMINING PLASMA MAGNESIUM BY FLAME SPECTROPHOTOMETRY.


THE CONSULTANT PHYSICIAN.

THE CONSULTANT PHYSICIAN.


THE TRAINING OF ORTHOPAEDIC SURGEONS.

THE TRAINING OF ORTHOPAEDIC SURGEONS.


INCREASE IN MEDICAL BENEFIT FUND CONTRIBUTIONS.


THE PROBLEM OF OBSERVER VARIATION IN MEDICAL DIAGNOSIS WITH SPECIAL REFERENCE TO CHEST DISEASES.


TOBACCO IN CARDIOVASCULAR DISEASE.

TOBACCO IN CARDIOVASCULAR DISEASE.


MEASLES VIRUS VACCINATION.

MEASLES VIRUS VACCINATION.


SPOROTRICHOSIS. AN IMPORTANT FUNGOUS DISEASE IN MINNESOTA.


NASAL HEMORRHAGE--THE TREATMENT.

NASAL HEMORRHAGE--THE TREATMENT.


THE TREATMENT OF ACROMEGALY.

THE TREATMENT OF ACROMEGALY.


ORAL CYTOLOGY.

ORAL CYTOLOGY.


MOSES THE FATHER OF PREVENTIVE MEDICINE.


COLLES' FRACTURE.

COLLES' FRACTURE.


PALLIATION IN METASTATIC CARCINOMA OF THE BREAST.


INDUSTRIAL MEDICINE. RELATIONSHIP OF OCCUPATIONAL MEDICINE.


ANTICOAGULANT THERAPY.

ANTICOAGULANT THERAPY.


CARDIAC DISEASE AND MATERNAL DEATHS IN MINNESOTA.


PORTLAND-1963. AMA CLINICAL MEETING ACTIONS REVIEWED.


RADIATION IN PREGNANCY.

RADIATION IN PREGNANCY.


EVALUATION OF A NEW BRONCHOGRAPHIC MEDIUM. AN EXPERIMENTAL STUDY.


CIRRHOSIS OF THE LIVER IN PREGNANCY. A CASE REPORT AND REVIEW OF THE LITERATURE.


CYCLIC MYOSITIS. A CORTISONE-RELATED VARIETY OF DERMATOMYOSITIS-POLYMYOSITIS.


DRUGS IN PREGNANCY.

DRUGS IN PREGNANCY.


HEMOBILIA DUE TO COMMON DUCT. HEPATIC ARTERY COMMUNICATION FOLLOWING OPERATIONS ON THE BILIARY TRACT.


PSEUDO-PSEUDOHYPOPARATHYROIDISM (BRACHYMETACARPAL DWARFISM). ENDOCRINOLOGIC STUDIES ON A PATIENT.


ACUTE LYMPHANGITIS AND CELLULITIS. DIAGNOSIS AND THERAPY.


A TRIUMPH OF ORGAN-GRAFTING TECHNIQUE AND ITS MORAL PROBLEMS.


TECHNICAL FACTORS IN THE X-RAY EXAMINATION OF THE CHEST.


REGIONAL ANESTHESIA IN CHILDREN WITH EXTREMITY INJURIES.


MULTIPLE SCLEROSIS.

MULTIPLE SCLEROSIS.


PROBLEMS IN DIAGNOSIS AND MANAGEMENT IN CARCINOMA OF THE BILE DUCTS.


INFECTIONS OF THE URINARY TRACT. II. SOME MICROBIOLOGIC CONSIDERATIONS.


PHEOCHROMOCYTOMA--PITFALLS IN DIAGNOSIS.


PROBLEMS IN ESTIMATING DISABILITY.

PROBLEMS IN ESTIMATING DISABILITY.


CERVICAL CYTOLOGY SURVEY.

CERVICAL CYTOLOGY SURVEY.


SEVERE ANEMIA IN THE NEWBORN.

SEVERE ANEMIA IN THE NEWBORN.


HARVEY CUSHING (1869-1939).

HARVEY CUSHING (1869-1939).


A LOCAL AUTHORITY PSYCHIATRIC HOSTEL.

A LOCAL AUTHORITY PSYCHIATRIC HOSTEL.


SALMONELLA TYPHI-MURIUM IN LONDON PIGEONS.


THE ISOLATION OF SALMONELLA TYPHI FROM SELENITE ENRICHMENT MEDIA.


IMMUNITY TO RABIES FOLLOWING VACCINATION.


THE EFFECT OF CARBON MONOXIDE ON THE HYDROXYLATION OF ANILINE AND N-ETHYLANILINE BY MICROSOMAL ENZYMES.


THE EFFECT OF ADRENALINE ON THE K AND NA FLUXES IN THE FROG'S ATRIUM.


PHYSIOLOGY OF BOWEL EVACUATION.

PHYSIOLOGY OF BOWEL EVACUATION.


REVIEW OF PHARMACOLOGY OF BOWEL EVACUANTS AND LAXATIVES.


INTRARECTAL PRESSURE AND THE URGE TO DEFECATE.


BOWEL EVACUATION IN INFANCY AND CHILDHOOD.


OBSTETRIC AND GYNECOLOGIC PROBLEMS OF BOWEL CONTENT ELIMINATION.


THE GERIATRIC AND BEDRIDDEN PATIENT AND THE LARGE BOWEL.


BOWEL PREPARATION FOR PROCTOSIGMOIDOSCOPY.


PANEL DISCUSSION--BOWEL EVACUATION.

PANEL DISCUSSION--BOWEL EVACUATION.


APLASTIC ANAEMIA. PRIZEWINNING NURSING CARE STUDY.


PULMONARY TUBERCULOSIS.

PULMONARY TUBERCULOSIS.


BACKGROUND TO ILLNESS.

BACKGROUND TO ILLNESS.


THE HEALTH VISITOR AS A CONTACT-TRACER IN TUBERCULOSIS.


TUBERCULOSIS AS A COMMUNITY PROBLEM.

TUBERCULOSIS AS A COMMUNITY PROBLEM.


PAY SLIPS.

PAY SLIPS.


THE PATIENTS GO TO TOWN. A CO-OPERATIVE EXPERIMENT IN REHABILITATING PSYCHIATRIC PATIENTS.


SOVIET ARTIFICIAL LIMBS.

SOVIET ARTIFICIAL LIMBS.


GOD AND HUMAN SUFFERING.

GOD AND HUMAN SUFFERING.


COMPULSORY GERIATRIC NURSING.

COMPULSORY GERIATRIC NURSING.


NEVER A DULL MOMENT-A SOLOMON ISLAND DIARY.


LUMBAR PUNCTURE.

LUMBAR PUNCTURE.


NURSES' HEALTH.

NURSES' HEALTH.


ENDOMETRIOSIS.

ENDOMETRIOSIS.


SPEECH THERAPY.

SPEECH THERAPY.


LOCAL AUTHORITY WELFARE WORK.

LOCAL AUTHORITY WELFARE WORK.


NURSING.

NURSING.


STATE REGISTRATION--A PROGRESS REPORT.

STATE REGISTRATION--A PROGRESS REPORT.


OCCUPATIONAL THERAPY.

OCCUPATIONAL THERAPY.


MEDICAL SOCIAL WORK.

MEDICAL SOCIAL WORK.


MANAGEMENT OF SPINAL INJURIES WITH ASSOCIATED CORD DAMAGE.


SURGICAL MANAGEMENT OF BENIGN LESIONS OF THE COLON.


COMMON STROKE SYNDROMES AND THEIR MANAGEMENT.


CONGENITAL HEART DISEASE WITHOUT A SHUNT. 1. COARCTATION OF THE AORTA AND ABERRANT CORONARY VESSELS.


TOTAL COLOR BLINDNESS AND ALBINISM. TWO CAUSES OF SUBNORMAL VISUAL ACUITY IN CHILDREN.


CLINICAL DISORDERS OF IRON UTILIZATION.

CLINICAL DISORDERS OF IRON UTILIZATION.


LONG-TERM MANAGEMENT OF DIABETES.

LONG-TERM MANAGEMENT OF DIABETES.


ATYPICAL CLINICAL SYNDROMES OF DUODENAL ULCER.


VINEBERG'S OPERATION FOR MYOCARDIAL ISCHEMIA.


"PUT NOT YOUR TRUST IN MONEY".

"PUT NOT YOUR TRUST IN MONEY".


MEDICAL PROBLEMS ASSOCIATED WITH PITUITARY SURGERY.


BRONCHIAL ASTHMA. INVENTORY AND OUTLOOK.


PREINVASIVE CARCINOMA OF THE CERVIX. ITS EARLY DETECTION AND MANAGEMENT.


DIABETIC AMYOTROPHY.

DIABETIC AMYOTROPHY.


PROBLEMS OF LEG LENGTH EQUALIZATION.

PROBLEMS OF LEG LENGTH EQUALIZATION.


A COMTEMPORARY VIEW OF UTERINE TUBAL PLASTIC SURGERY.


NUTRITIONAL PROBLEMS WITH MALIGNANT DISEASES. 2. SEQUELAE OF TREATMENT FOR  CANCER.


COUGH AND UNILATERAL PULMONARY DISEASE.

COUGH AND UNILATERAL PULMONARY DISEASE.


STROKES. 2. AMERICAN PRESIDENTS WHO HAD STROKES.


X-RAY DIAGNOSIS OF CONGENITAL CARDIAC DISEASE. 3. FIVE SPECIFIC LESIONS.


WILSON'S DISEASE (HEPATO-LENTICULAR DEGENERATION): A BRIEF REVIEW.


INSTITUTIONAL TREATMENT OF CHRONIC PSYCHOTIC PATIENTS.


PHYSICS IN THE TRAINING OF RADIOGRAPHERS. A RADIOLOGIST'S VIEW.


PHYSICS IN THE TRAINING OF RADIOGRAPHERS. A RADIOGRAPHER'S VIEW.


MACRORADIOGRAPHY IN THE DIAGNOSIS OF THE PNEUMOCONIOSES.


A TOXICOLOGIC STUDY OF DIMETHYL SULFOXIDE.


CHRONIC TOXICITY STUDIES ON IRRADIATED BEEF STEW AND EVAPORATED MILK.


COMPARATIVE STUDIES ON THE MORPHOLOGIC EFFECTS OF CALCIUM CARBIMIDE, PROPYLTHIOURACIL, AND DISULFIRAM IN MALE RATS.


RESPIRATORY STIMULATING PROPERTIES OF SOME DERIVATIVES OF INDANE.


STUDIES IN CARDIAC NECROSIS. I. PRODUCTION OF CARDIAC LESIONS WITH SYMPATHOMIMETIC AMINES.


TOXICITY OF INTRAVENOUSLY INJECTED URANIUM IN GUINEA PIGS.


RELATIVE NEPHROTOXIC PROPERTIES OF CHLORINATED METHANE, ETHANE, AND ETHYLENE DERIVATIVES IN MICE.


STUDIES ON THE TOXICOLOGIC AND PHARMACOLOGIC PROPERTIES OF THIABENDAZOLE.


ISCHEMIC ARM PAIN AND THE NONNARCOTIC ANALGESIC MEFENAMIC ACID, N-(2,3-XYLYL)ANTHRANILIC ACID.


EFFECT OF CHLORCYCLIZINE ON THE TOXICITY AND METABOLISM OF OCTAMETHYL PYROPHOSPHORAMIDE.


THE GROWTH, REPRODUCTION, LONGEVITY, AND HISTOPATHOLOGY OF RATS FED GAMMA-IRRADIATED FLOUR.


SOLUBILITY OF BENZENE HEXACHLORIDE ISOMERS IN RAT FAT.


STUDIES USING CLOSTRIDIUM BOTULINUM TOXIN--TYPE A.


THE ANTAGONISM OF MONOAMINE OXIDASE (MAO) INHIBITORS BY THE RED BLOOD CELL AND ITS CONSTITUENTS.


DISTRIBUTION OF C14-DDT AND C14-DIELDRIN IN PREGNANT MICE DETERMINED BY WHOLE-BODY AUTORADIOGRAPHY.


STUDIES IN CARDIAC NECROSIS. II. CARDIOVASCULAR EFFECTS OF SYMPATHOMIMETIC AMINES PRODUCING CARDIAC LESIONS.


SOME RESULTS OF ENZYMATIC AND CHROMOSOMAL ABNORMALITIES.


PNEUMOCYSTIS CARINII PNEUMONIA WITH HYPOGAMMAGLOBULINEMIA; (CASE REPORT).


A SCHOOL CONSERVATION OF HEARING PROGRAM.


THE ROLE OF THE GENERAL PRACTITIONER.

THE ROLE OF THE GENERAL PRACTITIONER.


PRESCRIPTION LABELING--PRO AND CON.

PRESCRIPTION LABELING--PRO AND CON.


STUDIES ON HYPOXIA I: THE RESPONSE OF THE BONE MARROW TO PRIMARY HYPOXIA.


HISTOCHEMICAL DEMONSTRATION OF SOME AMINO ACIDS IN THE TOOTH GERM OF THE RAT'S UPPER INCISORS.


SURGICAL TREATMENT OF ATRIAL SEPTAL DEFECT OF ANTERIOR TYPE WITH THE AID OF EXTRACORPOREAL CIRCULATION.


RUPTURED AND DISSECTING ANEURYSMS OF THE AORTA. INCIDENCE AND PROSPECTS OF SURGERY.


SOME UNANSWERED QUESTIONS CONCERNING ENLARGEMENT AND FAILURE OF THE HEART.


CLINICAL INTERPRETATION OF ELECTROCARDIOGRAM BY MEANS OF ELECTRONIC COMPUTERS.


PERI-INFARCTION BLOCK: POSTMYOCARDIAL-INFARCTION INTRAVENTRICULAR CONDUCTION DISTURBANCE.


MYOCARDIAL DEGENERATION IN CARDIAC SARCOIDOSIS: HISTOCHEMICAL AND ELECTRON MICROSCOPIC STUDIES.


EFFECT OF CONCENTRATED CONTRAST MEDIA DURING ANGIOGRAPHY ON PLASMA VOLUME AND PLASMA OSMOLALITY.


COMPLICATIONS IN 4,413 CATHETERIZATIONS OF THE RIGHT SIDE OF THE HEART.


ELECTROCARDIOGRAPHIC CHANGES DURING DAILY WORK AND ACUTE EXERCISE IN PERMANENT INHABITANTS OF HOT AREAS.


TRIAMTERENE: CLINICAL STUDIES IN ARTERIAL HYPERTENSION.


BERIBERI HEART DISEASE: PHYSIOLOGIC DATA AND DIFFICULTIES IN DIAGNOSIS.


ELEVATION OF SERUM CHOLESTEROL AFTER ADMINISTRATION OF NICOTINE.


CHANGES IN AMINO ACID COMPOSITION OF AORTIC ELASTIN WITH AGING AND ATHEROSCLEROSIS.


EFFECT OF L-NOREPINEPHRINE ON RIGHT ATRIAL PRESSURE AND THE "SQUARE WAVE RESPONSE" TO THE VALSALVA MANEUVER.


EFFECT OF 50 PER CENT SODIUM DIATRIZOATE (HYPAQUE) ON BLOOD FLOW IN THE INTERNAL CAROTID ARTERY OF MAN.


LYMPHANGIOGRAPHY. AN IMPROVED TECHNIQUE OF LYMPHATIC CANNULATION.


HEMODYNAMIC EFFECTS OF RAPID DIGITALIZATION IN EXPERIMENTAL CARDIOGENIC SHOCK.


EFFECT OF SITE OF INJECTION UPON LEFT VENTRICULAR INDICATOR-DILUTION OUTPUT.


OBSERVATIONS ON THE LOCALIZATION OF TRITIATED DIGOXIN IN MYOCARDIAL CELLS BY AUTORADIOGRAPHY AND ULTRAMICROSCOPY: WITH ADDED REFERENCE TO THE SARCOTUBULAR SYSTEM STUDIED BY CELL FRACTIONATION AND ENZYME STAINING.


MYOCARDIAL INFARCTION, GRAM-NEGATIVE BACTEREMIA, PROLONGED SHOCK, AND LEUKEMOID REACTION.


HYPOKALEMIC ALKALOSIS WITH ADRENAL TUMOR: A CASE REPORT. CONN'S SYNDROME OR DRUG-INDUCED HYPOKALEMIC ALKALOSIS.


CLINICAL PATHOLOGIC CONFERENCE.

CLINICAL PATHOLOGIC CONFERENCE.


RESTRICTIVE CARDIAC DISEASE. PERICARDITIS AND THE MYOCARDIOPATHIES.


DIURETIC THERAPY. IX. ETHACRYNIC ACID.

DIURETIC THERAPY. IX. ETHACRYNIC ACID.


CINEANGIOCARDIOGRAPHY IN HEMORRHAGIC SHOCK.


VICTIMS OF THE VECTOR.

VICTIMS OF THE VECTOR.


CONTINUOUS ELECTROCARDIOGRAPHIC MONITORING IN ACUTE MYOCARDIAL INFARCTION.


THE SERUM GROUP SPECIFIC COMPONENT IN NONHUMAN PRIMATES.


RESPIRATORY STIMULANTS IN OBSTRUCTIVE LUNG DISEASE.


THE CRITICAL ROLE OF CHLORIDE IN THE CORRECTION OF HYPOKALEMIC ALKALOSIS IN MAN.


BRONCHIOLECTASIS: THE CLINICAL SYNDROME AND ITS RELATIONSHIP TO CHRONIC LUNG DISEASE.


THE REGULATION OF VENTILATION IN DIFFUSE PULMONARY FIBROSIS.


MECHANISMS OF CHRONIC CARBON DIOXIDE RETENTION IN PATIENTS WITH OBSTRUCTIVE PULMONARY DISEASE.


COR PULMONALE IN CYSTIC FIBROSIS: A MORPHOMETRIC ANALYSIS.


VON RECKLINGHAUSEN'S NEUROFIBROMATOSIS ASSOCIATED WITH CYSTIC LUNG DISEASE.


ADENOCARCINOMA OF THE HEPATIC DUCT AT ITS BIFURCATION WITHIN THE PORTA HEPATIS. AN UNUSUAL TUMOR WITH DISTINCTIVE CLINICAL AND PATHOLOGICAL FEATURES.


CONGENITAL HEMOLYTIC JAUNDICE CORRECTED BY SPLENECTOMY FOLLOWED TWENTY-SIX YEARS LATER BY BLEEDING TENDENCY, HEPATOMEGALY AND SUDDEN DEATH.


PROMINENCE OF THE LEFT EPIPERICARDIAL FAT PAD. A CAUSE OF APPARENT CARDIOMEGALY.


SIDEROPENIC DYSPHAGIA IN A PATIENT WITH ULCERATIVE COLITIS.


PULMONARY ARTERY OBSTRUCTION AND COR PULMONALE DUE TO CHRONIC FIBROUS MEDIASTINITIS.


AN UNUSUAL SERUM LIPOPROTEIN-GLOBULIN COMPLEX IN A PATIENT WITH HYPERLIPEMIA.


THE SYNDROME OF BENIGN RECURRENT CHOLESTASIS.


ASYMPTOMATIC HYPERNATREMIA: PHYSIOLOGICAL AND CLINICAL STUDY.


SCUTARI, U.S.A.

SCUTARI, U.S.A.


PRIMARY MYOCARDIAL DISEASE IN MAN RESULTING FROM ACUTE CARBON MONOXIDE POISONING.


DRUGS IN PREGNANCY.

DRUGS IN PREGNANCY.


RESOLVING A NURSING LEADERSHIP CRISIS.

RESOLVING A NURSING LEADERSHIP CRISIS.


RAISING SPUTUM SPECIMENS.

RAISING SPUTUM SPECIMENS.


ALCOHOLISM.

ALCOHOLISM.


ALCOHOLISM: CARING FOR THE ALCOHOLIC ON A MEDICAL UNIT.


WHO DOES WHAT--IN DEFENSE, IN NATURAL DIASTER.


STUDENT, INSTRUCTOR, AND LIABILITY.

STUDENT, INSTRUCTOR, AND LIABILITY.


ONE DIAGNOSIS--FOUR PATIENTS.

ONE DIAGNOSIS--FOUR PATIENTS.


EVENING NURSES IMPROVE THEIR PRACTICE.

EVENING NURSES IMPROVE THEIR PRACTICE.


THE LAST STAGES OF LIFE.

THE LAST STAGES OF LIFE.


SURGICAL TREATMENT OF HIP INJURIES.

SURGICAL TREATMENT OF HIP INJURIES.


THE NURSE'S ROLE IN CLOSED CHEST CARDIAC RESUSCITATION.


NEW CHART FORMS SOLVE OLD PROBLEMS.

NEW CHART FORMS SOLVE OLD PROBLEMS.


NURSING IN THE OAK-CHIP EXPERIMENT.

NURSING IN THE OAK-CHIP EXPERIMENT.


EXPERIMENTAL THYROIDITIS IN THE GUINEA PIG. II. ELECTRON MICROSCOPY.


THE EFFECT OF BACTERIAL ENDOTOXINS ON TOXIC CORNEAL LESIONS PRODUCED BY INFLUENZA AND NEWCASTLE DISEASE VIRUSES.


SULFATED MUCOPOLYSACCHARIDE IN FIBRINOID GLOMERULAR OCCLUSIONS OF THE GENERALIZED SHWARTZMAN REACTION.


TOXIC MYODEGENERATION IN GOATS PRODUCED BY FEEDING MATURE FRUITS FROM THE COYOTILLO PLANT (KARWINSKIA HUMBOLDTIANA).


DISTRIBUTION AND SPECIES LIMITATION OF THE ADRENAL LESIONS INDUCED BY 7,12-DIMETHYLBENZ(A)ANTHRACENE.


THE RELATIONSHIP BETWEEN ADENOSINETRIPHOSPHATASE ACTIVITY AND TRIETHYLTIN TOXICITY IN THE PRODUCTION OF CEREBRAL EDEMA OF THE RAT.


FINE-STRUCTURE CHANGES IN ACHALASIA OF THE ESOPHAGUS. I. VAGUS NERVES.


AMYLOIDOSIS AND LYMPHOID APLASIA IN MOUSE RADIATION CHIMERAS.


SUBMICROSCOPIC RESPONSE OF ARTICULAR CARTILAGE OF MICE TREATED WITH ESTROGENIC HORMONE.


CIRCULATORY RESPONSES TO HYPOCAPNIA IN THE ANESTHETIZED DOG.


PREDICTION VALUES FOR SCREENING TESTS OF PULMONARY FUNCTION.


THE FINE STRUCTURE OF HEPATIC CELLS IN CHRONIC ETHIONINE POISONING AND DURING RECOVERY.


SURGICAL CONSIDERATIONS IN PULMONARY TUBERCULOSIS COMPLICATED BY BRONCHOPULMONARY ASPERGILLOSIS.


HISTOCHEMICAL DETECTION OF MUCOPOLYSACCHARIDES IN THE MAJOR SALIVARY GLANDS OF THE RAT.


SURGERY FOR ADULT CONGENITAL CARDIOVASCULAR DISEASE.


AORTIC AND MITRAL VALVE REPLACEMENTS. INDICATIONS AND RESULTS.


COARCTATION OF THE AORTA IN ASSOCIATION WITH ATRIAL SEPTAL DEFECT.


DIAGNOSIS AND SURGICAL TREATMENT OF HEART DISEASE IN ARIZONA.


OPEN VALVULOTOMY FOR MITRAL STENOSIS.

OPEN VALVULOTOMY FOR MITRAL STENOSIS.


RESULTS FOLLOWING TOTAL VALVE REPLACEMENT FOR ACQUIRED HEART DISEASE.


THE SURGERY OF MITRAL VALVULAR DISEASE.

THE SURGERY OF MITRAL VALVULAR DISEASE.


SELECTIVE REINFORCEMENT OF SCHIZOPHRENICS' INTERVIEW RESPONSES.


ON THE SPECIFICITY OF BACTERIOPHAGE-INDUCED HYDROXYMETHYLCYTOSINE GLUCOSYLTRANSFERASES. I. SPECIFICITY DIFFERENCE BETWEEN THE HYDROXYMETHYLCYTOSINE ALPHA-GLUCOSYLTRANSFERASES INDUCED BY BACTERIOPHAGES T2, T4 AND T6.


STUDIES ON THE MECHANISM OF INTESTINAL ABSORPTION OF SUGARS. VII. PHENYLGLYCOSIDE TRANSPORT AND ITS POSSIBLE RELATIONSHIP TO PHLORIZIN INHIBITION OF THE ACTIVE TRANSPORT OF SUGARS BY THE SMALL INTESTINE.


LOCALIZING THE RETINAL HOLE IN THE SURGICAL TREATMENT OF RETINAL DETACHMENT.


THE FORMATION OF (14C)-HISTAMINE IN VIVO IN NORMAL RATS AND IN RATS TREATED WITH LIOTHYRONINE. Female rats treated with liothyronine or its solvent (control rats) were given an intravenous injection of [(14)C]-L-histidine. The amount of [(14)C]-histamine in various tissues was measured at 10 min, 60 min and 22 hr after the injection. In control rats the glandular stomach contained large amounts of [(14)C]-histamine at 10 and 60 min, with a sharp decline at 22 hr. The skin contained small amounts at 10 and 60 min, with a slight rise at 22 hr. In rats treated with liothyronine there was more [(14)C]-histamine in the stomach at 10 and 60 min, but not at 22 hr after the injection of [(14)C]-L-histidine. The results support the following conclusions: (1) The glandular stomach in the intact rat forms more histamine than do other tissues; the gastric histamine is in a rapid state of turnover and is likely to contribute substantially to the urinary output of histamine. (2) After treatment with liothyronine the rat has an increased histamine formation in the stomach; this is probably the main cause of the raised urinary histamine excretion in such a rat.


KININ-FORMING AND DESTROYING ACTIVITIES OF SALIVA. Kininase activity is present in saliva obtained from the oral cavity but not in saliva obtained by cannulation of the parotid or submandibular ducts. This activity originates from squamous epithelial cells from the oral mucous membranes. Experiments with various inhibitors indicated that the kininase activity of squamous epithelial cells differs from that of plasma, and resembles that of erythrocytes. Kinin-forming activity is present both in the glandular secretion and in the squamous epithelial cells.


POSSIBLE RELATIONSHIP BETWEEN TETRACYCLINE STABILITY AND EFFECT ON FOETAL SKELETON. A possible relationship between stability of a tetracycline and persistence in the foetal skeleton is discussed; it seems that the greater the stability of a tetracycline, the more it may interfere with bone-forming processes. Therefore, assuming that limited instability does not impair antibiotic potency in vivo, the use of a low-stability tetracycline seems to be a safer form of treatment in order to avoid undesired effects on the foetal skeleton.


SYMPATHOLYTIC DRUGS AND HYPERLIPAEMIA INDUCED IN RATS BY INTRAPERITONEAL INJECTIONS OF SURFACE-ACTIVE AGENT. Hyperlipaemia and hypercholesterolaemia were induced in white rats by intraperitoneal injections of tyloxapol. Various sympatholytics and adrenolytics, including blocking agents of beta-receptors, were given simultaneously with tyloxapol. Bretylium tosylate prevented the increase in the serum levels of esterified fatty acids and cholesterol caused by tyloxapol. Phentolamine decreased the enhancement by tyloxapol of cholesterol and total lipid concentrations in the serum. Guanethidine and phenoxybenzamine reduced the increase in the concentration of esterified fatty acids, whereas dichloroisoprenaline insignificantly increased the tyloxapol-induced hyperlipaemia. A small dose of pronethalol slightly increased the esterified fatty acid level in tyloxapol-treated animals; a large dose significantly decreased the serum cholesterol concentration.


COBALT COMPOUNDS AS ANTIDOTES FOR HYDROCYANIC ACID. The antidotal potency of a cobalt salt (acetate), of dicobalt edetate, of hydroxocobalamin and of cobinamide against hydrocyanic acid was examined mainly on mice and rabbits. All the compounds were active antidotes for up to twice the LD50; under some conditions for larger doses. The most successful was cobalt acetate for rabbits (5xLD50), which was effective at a molar cyanide/cobalt (CN/Co) ratio of 5, but had as a side-effect intense purgation. Hydroxocobalamin was irregular in action, but on the whole was most effective for mice (4.5xLD50 at a molar ratio of 1), and had no apparent side effects. Dicobalt edetate, at molar ratios of up to 2, was more effective for rabbits (3xLD50) than for mice (2xLD50), but had fewer side effects than cobalt acetate. The effect of thiosulphate was to augment the efficacy of dicobalt edetate and, in mice, that of hydroxocobalamin; but, apparently, in rabbits, to reduce that of hydroxocobalamin. Cobinamide, at a molar ratio of 1, was slightly more effective than hydroxocobalamin on rabbits and also less irregular in its action. Cobalt acetate by mouth was effective against orally administered hydrocyanic acid. The oxygen uptake of the body, reduced by cyanide, is rapidly reinstated when one of the cobalt antidotes has been successfully administered.


INHIBITORS OF HISTAMINE CATABOLISM AND THE ACTION OF GASTRIN IN THE RAT. A method is described for the partial purification of hog's antral gastrin by gel filtration and ion-exchange chromatography. Gastrin was assayed by its effect on the pH of the effluent fluid from the perfused lumen of the stomach of the anaesthetized rat. The latency of the response to gastrin given intravenously was shorter than the latency to a similar dose of histamine. The response to gastrin injected into the arterial circulation of the stomach appeared sooner than the response to gastrin injected intravenously. Iproniazid and aminoguanidine potentiated responses to gastrin. Incubation in vitro with monamine oxidase or diamine oxidase did not inactivate gastrin. Chlorpromazine and bromolysergic acid diethylamide, in doses which enhance the effects of histamine on acid gastric secretion, did not affect responses to gastrin.


THE ADRENERGIC-NEURONE BLOCKING ACTION OF SOME COUMARAN COMPOUNDS. Ethyldimethyl(7-methylcoumaran-3-yl)ammonium iodide (SK&F 90,109) and its guanidine analogue [N-(7-methylcoumaran-3-yl)guanidine nitrate] (SK&F 90,238) abolish the effects of adrenergic nerve stimulation in cats, as do xylocholine and bretylium. SK&F 90,109 has slight sympathomimetic actions; these are less marked than in SK&F 90,238. Large doses of SK&F 90,109 have an action, dependent on local noradrenaline stores, that delays the appearance of adrenergic-neurone blockade in conscious cats. Responses to adrenaline are, in general, enhanced by each drug, but SK&F 90,238 transiently antagonizes tachycardia induced by adrenaline and isoprenaline. Both drugs inhibit the release of noradrenaline from the spleen during splenic nerve stimulation, but the release of catechol amines from the adrenal glands, in response to electrical or chemical stimulation, is unimpaired. In contrast to the prolonged adrenergic-neurone blocking action, any inhibition of the effects of cholinergic nerve stimulation is transient. Large intravenous doses produce neuromuscular blockade. The compounds have a slight central depressant action. In contrast to reserpine and guanethidine the noradrenaline content of rat hearts is not appreciably lowered 24 hr after a single dose of either drug. Unlike xylocholine they are not local anaesthetics. Related compounds also block the effects of adrenergic-nerve stimulation. The possible modes of action of these drugs are discussed.